LOCUS       DAZPDU010000001        80165 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZPDU010000001 DAZPDU010000000
VERSION     DAZPDU010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 80165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80165
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.001"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            75..359
                     /gene="pduA"
                     /locus_tag="ACHURS_000001"
     CDS             75..359
                     /gene="pduA"
                     /locus_tag="ACHURS_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduA"
                     /protein_id="HFR1235846.1"
                     /translation="MQQEALGMVETKGLTAAIEAADAMVKSANVLLVGYEKIGSGLVT
                     VIVRGDVGAVKAATDAGAAAAHNVGEVKAVHVIPRPHTDVEKILPKGINP"
     gene            356..1168
                     /gene="pduB"
                     /locus_tag="ACHURS_000002"
                     /pseudo
     CDS             356..1168
                     /gene="pduB"
                     /locus_tag="ACHURS_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228567.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduB"
     gene            1187..2851
                     /gene="pduC"
                     /locus_tag="ACHURS_000003"
     CDS             1187..2851
                     /gene="pduC"
                     /locus_tag="ACHURS_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase large subunit PduC"
                     /protein_id="HFR1235847.1"
                     /translation="MRSKRFEALAKRPVNQDGFVKEWIEEGFIAMESPNDPKPSIKIV
                     NGVVTELDGKPASQFDLIDHFIARYGINLARAEEVIAMDSVKLANMLCDPNVKRSDIV
                     PLTTAMTPAKIVEVVSQMNVVEMMMAMQKMRARRTPSQQAHVTNVKDNPVQIAADAAE
                     GAWRGFDEQETTVAVARYAPFNAIALLVGSQVGRPGVLTQCSLEEATELKLGMLGHTC
                     YAETISVYGTEPVFTDGDDTPWSKGFLASSYASRGLKMRFTSGSGSEVQMGYAEGKSM
                     LYLEARCIYITKAAGVQGLQNGSVSCIGVPSAVPSGIRAVLAENLICSSLDLECASSN
                     DQTFTHSDMRRTARFLMQFLPGTDFISSGFSAVPNYDNMFAGSNEDAEDFDDYNVIQR
                     DLKVDGGLRPVREEDVIAIRNKAARALQAVFAGMGLPPITDEEVEAATYAHGSKDMPE
                     RNIVEDIKFAQEIINKNRNSLEVVKALAQGGFTDVAQDMLNMQKAKLTGDYLHTSAII
                     VDDGQVLSAVNDVNDYAGPATGYRLQGERWEEIKNIPGALDPNEID"
     gene            2861..3529
                     /locus_tag="ACHURS_000004"
     CDS             2861..3529
                     /locus_tag="ACHURS_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase medium
                     subunit"
                     /protein_id="HFR1235848.1"
                     /translation="MEINEKLLRQIIEDVLAEMQPSDKSVSFRAPVSATVPSAPDTGN
                     FLTEIGEAQQGTQQDEVIIAVGPAFGLAQTVNIIGIPHKNILREVIAGIEEEGIKARV
                     IRCFKSSDVAFVAVEGDRLSGSGIAIGIQSKGTTVIHQQGLPPLSNLELFPQAPLLTL
                     DTYRQIGKNAARYAKRESPQPVPTLNDQMARPKYQAKSAILHIKETKYVVTGKKPQEL
                     RVTF"
     gene            3544..4062
                     /gene="pduE"
                     /locus_tag="ACHURS_000005"
     CDS             3544..4062
                     /gene="pduE"
                     /locus_tag="ACHURS_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase small subunit PduE"
                     /protein_id="HFR1235849.1"
                     /translation="MNTDAIESMVRDVLNRMNSLQDAAPVSAVPNASILSAKVTDYPL
                     ANKHPEWVKTATNKTLDDFTLENVLSDNVTALDMRITPETLRIQAAIARDAGRDRLAM
                     NFERAAELTSVPDDRILEIYNALRPYRSTKQELIAIADDLEQRYQAKICAAFVREAAE
                     LYVERKKLKGDD"
     gene            4072..5904
                     /locus_tag="ACHURS_000006"
     CDS             4072..5904
                     /locus_tag="ACHURS_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase reactivase subunit alpha"
                     /protein_id="HFR1235850.1"
                     /translation="MRYIAGIDIGNSSTEVALATLSATGELSFVSSALEETTGIKGTL
                     RNVHGIQEALAQATKKVGINVSDISLIRINEATPVIGDVAMETITETIITESTMIGHN
                     PKTPGGVGLGVGFTITPQELLTRPADTPYILVVSSAFDFADIATMINASVRAGYQLTG
                     VILQRDDGVLVNNRLEIPLPIVDEVLYIDRIPLGMLAAIEVAVPGKVIETLSNPYGIA
                     TVFALNAEETKNIVPVARALIGNRSAVVVKTPSGDVKARSIPAGNIELLSAGRTTRVD
                     VAAGADAIMKAVGECPKLENVTGEPGTNIGGMLEHVRQTMAELTNKPSNEIFIQDLLA
                     IDTSVPVSVTGGLAGEFSLEQAVGIASMVKSDRLQMAMIASEIKQKLHVDVQVGGAEA
                     EAAIQGALTTPGTTRPLAILDLGAGSTDASIINQSGEIVATHLAGAGDMVTMIIAREL
                     GLNDRYLAEEIKKYPLAKVESLFHLRHEDGSVQFFPTPLSPHVFARVCVVKPDELVPI
                     PGDLTLEKVRAVRRSAKERVFVTNALRALRQVSPAGNIRDIPFVVLVGGSSLDFEVPQ
                     LVTDALAHYRLVAGRGNIRGSEGPRNAVATGLLIAWHKESIHGK"
     gene            5894..6244
                     /locus_tag="ACHURS_000007"
     CDS             5894..6244
                     /locus_tag="ACHURS_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016153531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydratase reactivase beta/small
                     subunit family protein"
                     /protein_id="HFR1235851.1"
                     /translation="MESKERAPAIVVMEIGDCITTWDEVLLGIEEEGIPFRIQHIPSG
                     EVIDSAWLAARQSPLLVGIACDREELIVHYKNLPASAPLFTLTYQQDNHTRRSIGTNA
                     ARLVKGIPFREFHS"
     gene            6264..6539
                     /gene="pduJ"
                     /locus_tag="ACHURS_000008"
     CDS             6264..6539
                     /gene="pduJ"
                     /locus_tag="ACHURS_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduJ"
                     /protein_id="HFR1235852.1"
                     /translation="MNNALGLVETKGLVGAIEAADAMVKSANVQLVGYEKIGSGLITV
                     MVRGDVGAVKAAVDAGSAAASAVGEVKSCHVIPRPHSDVEAILPKSA"
     gene            6563..6985
                     /locus_tag="ACHURS_000009"
     CDS             6563..6985
                     /locus_tag="ACHURS_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HFR1235853.1"
                     /translation="MKQSLGLLEASGLALAISCADAMAKAASITLVGLEKTNGSGWTL
                     IKITGDVASVNAAIATGASFAGQRNGLVAHKVIARPGDGILPHTTEALTVTVETSKTP
                     ATTVTSEIISCNLCLDPACQRQKGEPRSLCLYANKRGE"
     gene            6988..7620
                     /locus_tag="ACHURS_000010"
     CDS             6988..7620
                     /locus_tag="ACHURS_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HFR1235854.1"
                     /translation="MDKQTLQETVSQILDEMRERPIPLGVSSRHIHLSSADYLRLFPE
                     QPISEKKPLMQPGQYAAEQVVTLEGPKGKLHNVRLLGPLRNASQVEISRTDARTLGIT
                     APLRMSGDLSGTPGIRLISPFAELEITSGVIVAQRHIHMSPLDALIYGVTHGDKVAVA
                     IEGDERRLIFENVAVRVSPDMRLEMHIDTDEANAAGADSPDVFATLVSVP"
     gene            7617..8108
                     /gene="pduM"
                     /locus_tag="ACHURS_000011"
     CDS             7617..8108
                     /gene="pduM"
                     /locus_tag="ACHURS_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcompartment protein PduM"
                     /protein_id="HFR1235855.1"
                     /translation="MNNMLMQRIVDEVVFRLKQRAGKTLVLTVFQLRDASVQESVHQY
                     ASLQIRYVDLPLLRQLAENETSDRASIQIHEALAWGMHIQLSLQRHFLNAIELKTLAR
                     LPLSWCDEQGQPIYLHHNRLLSYADIAQLSSGILVLQRKCCVTALAREAAITRNIQLI
                     RQE"
     gene            8112..8387
                     /gene="pduN"
                     /locus_tag="ACHURS_000012"
     CDS             8112..8387
                     /gene="pduN"
                     /locus_tag="ACHURS_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduN"
                     /protein_id="HFR1235856.1"
                     /translation="MHLARVTGAVVSTQKSPSLIGKKLLLVRRVSADGELPLTPLTGD
                     EVAVDSVGAGVGELVLLSSGSSARHVFSGPNEAIDLAVVGIVDTLSR"
     gene            8399..9406
                     /gene="pduO"
                     /locus_tag="ACHURS_000013"
     CDS             8399..9406
                     /gene="pduO"
                     /locus_tag="ACHURS_000013"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-domain cob(I)yrinic acid a,c-diamide
                     adenosyltransferase PduO"
                     /protein_id="HFR1235857.1"
                     /translation="MAIYTRTGDSGSTSLFTGQRVSKTHLRVETYGTLDELNATLSLC
                     YCATAIESHRILLEAIQQQIFWFSAELASESEQPSAQQRYIGTEEIAALENAIDSAMN
                     AVPPVHSFILPGRCEAASRMHFARTVARRAERRLVELTTETTVRNVLLHYINRLSDCL
                     YALARVEDNVAHQNLMIQEITKRYHEANHTPALKERTMPLTFQDLHQLIRSAAMRADE
                     LHIPVVISIVDANGTESVTWRMPDALLVSSELAPKKAWTAVAMKTATHKLADTVQPDA
                     PLYGLESHMQGKVVTFGGGFPLWRDGKLLGGLGISGGSVEQDMDIAQSAMAAINVGVN
                     Q"
     gene            9403..10788
                     /gene="pduP"
                     /locus_tag="ACHURS_000014"
                     /pseudo
     CDS             9403..10788
                     /gene="pduP"
                     /locus_tag="ACHURS_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460996.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating propionaldehyde dehydrogenase
                     PduP"
     gene            10799..11911
                     /locus_tag="ACHURS_000015"
     CDS             10799..11911
                     /locus_tag="ACHURS_000015"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010835540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0051144 - propanediol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-propanol dehydrogenase PduQ"
                     /protein_id="HFR1235858.1"
                     /translation="MNTFSLQTRLYSGPGSLKVLSRFSHRHIWIICDAFLARSPLIDT
                     LHQALPDSNRLSIFSEITPAPTIQTVVKGIAQMQTLRPDVVIGFGGGSALDAAKAIVW
                     FGRQCGIEIETCVAIPTTSGIGSEVTSACVISDPEKGIKYPLFDNALYPDIAILDPSL
                     VVSVPPAITANTGMDVLTHALEAYVSPRASDFTDALVEKAVQIVFQYLPTAVKKGDCL
                     ATRGKMHNASTLAGMAFSQAGLGLNHAIAHQLGGQFHLPHGLANALLLTAVIRFNAGD
                     PRTAKRYARLAKTCHLCPDNANDTASLNALIQHIEQLNTTCTLPTLANALKEKKAEWS
                     IRIPDMVQAALADATLRTNPRAADASAIAELLEELL"
     gene            11908..13251
                     /locus_tag="ACHURS_000016"
     CDS             11908..13251
                     /locus_tag="ACHURS_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR1235859.1"
                     /translation="MNVGLISCDDQTIRERVQAAGVVGAGGAGFPTHIKLQARADTFL
                     VNAAECEPMLKVDQQLMAQQASRLLRGVHYAMKATGASSGIIALKEKYQRAINALTPL
                     LPPDIRLHILPDVYPAGDEVLTIWMATGRRVPPAALPVSVGVVVNNVQTVLNIARAVE
                     QQYPVTRRTLTVNGAVAKPITVTVPIGMSLREVLALAGGATVDAPGFINGGPMMGSLI
                     TSLDTPVSKTTGGLLVLPNSHSLIQRRMQNDRSVLAVAKTVCEQCRLCTDLCPRHLIG
                     HELAPHLLVRAVNYQQLATPQLLLTALTCSECNVCASVACPVGISPMRINRLLKQELR
                     AQNLRYEGALNPADPMANYRLIPVKRLVTRLGLTPWYQDAPLSEQVPQPEKVTLLLRQ
                     HIGASAIACVQKGDRVVHGQCVGQIPHGTLGAPIHASIDGMVSDVTENAITLVRG"
     gene            13254..13808
                     /gene="pduT"
                     /locus_tag="ACHURS_000017"
     CDS             13254..13808
                     /gene="pduT"
                     /locus_tag="ACHURS_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduT"
                     /protein_id="HFR1235860.1"
                     /translation="MSKAVGILELSSIAKGMETGDAMLKSANVELLVSKTLCPGKFLL
                     MLGGDVGAVQQAINSGASLAGEMLVDSLVLPNIHPSLLSAISGLNHVEQHRAVGVVET
                     WSVAACIDAADCAVKAANVILVRVHMAFGIGGKCYMVVAGDISDVNNAVSAASVRAGE
                     KGLLVYRSVIARPHEAMWRQMVEG"
     gene            13808..14158
                     /gene="pduU"
                     /locus_tag="ACHURS_000018"
     CDS             13808..14158
                     /gene="pduU"
                     /locus_tag="ACHURS_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000441103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduU"
                     /protein_id="HFR1235861.1"
                     /translation="MTTQHTTERMIQEYVPGKQVTLAHVIANPGKDLFKKLGLPDTIS
                     AIGILTITPSEASIIACDIATKSGAVEIGFLDRFTGAVVLTGDVSAIEYALSQVTRTL
                     GEMMRFTACPITRT"
     gene            14163..14606
                     /locus_tag="ACHURS_000019"
     CDS             14163..14606
                     /locus_tag="ACHURS_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFR1235862.1"
                     /translation="MQRMMLIGPSQCGKTSLTQCLRGENIHYQKTQAIVWSPTTIDTP
                     GEYLENRSLYSALLVSACEADIIALVLNADAPWSPFSPGFTSSMNRPVIGVVTKADLA
                     NMEQISLVKCWLREAGAHNVLVTSAVNNHGVAELLALLHTEEGCR"
     gene            complement(14707..15036)
                     /locus_tag="ACHURS_000020"
     CDS             complement(14707..15036)
                     /locus_tag="ACHURS_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFR1235863.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(15208..16266)
                     /locus_tag="ACHURS_000021"
     CDS             complement(15208..16266)
                     /locus_tag="ACHURS_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFR1235864.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSFVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(16464..16937)
                     /gene="sbmC"
                     /locus_tag="ACHURS_000022"
     CDS             complement(16464..16937)
                     /gene="sbmC"
                     /locus_tag="ACHURS_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFR1235865.1"
                     /translation="MNYEITQEEKRTVAGFHLVGPWEQTVKKGFEQLMMWVDNKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(17056..18222)
                     /gene="dacD"
                     /locus_tag="ACHURS_000023"
     CDS             complement(17056..18222)
                     /gene="dacD"
                     /locus_tag="ACHURS_000023"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFR1235866.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGGMFSRLSDYFHHKA"
     gene            18431..19858
                     /gene="sbcB"
                     /locus_tag="ACHURS_000024"
     CDS             18431..19858
                     /gene="sbcB"
                     /locus_tag="ACHURS_000024"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFR1235867.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADNKEKVALLKALWQYAEEIV"
     gene            complement(19901..20128)
                     /gene="tsuB"
                     /locus_tag="ACHURS_000025"
     CDS             complement(19901..20128)
                     /gene="tsuB"
                     /locus_tag="ACHURS_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFR1235868.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(20142..21200)
                     /gene="tsuA"
                     /locus_tag="ACHURS_000026"
     CDS             complement(20142..21200)
                     /gene="tsuA"
                     /locus_tag="ACHURS_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFR1235869.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAEAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCFIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(21379..22737)
                     /gene="plaP"
                     /locus_tag="ACHURS_000027"
     CDS             complement(21379..22737)
                     /gene="plaP"
                     /locus_tag="ACHURS_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFR1235870.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(22727..22789)
                     /gene="yoeI"
                     /locus_tag="ACHURS_000028"
     CDS             complement(22727..22789)
                     /gene="yoeI"
                     /locus_tag="ACHURS_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFR1235871.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(23004..23933)
                     /locus_tag="ACHURS_000029"
     CDS             complement(23004..23933)
                     /locus_tag="ACHURS_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR1235872.1"
                     /translation="MKQLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLVQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(23979..24803)
                     /locus_tag="ACHURS_000030"
     CDS             complement(23979..24803)
                     /locus_tag="ACHURS_000030"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR1235873.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(24886..25140)
                     /gene="yoeB"
                     /locus_tag="ACHURS_000031"
     CDS             complement(24886..25140)
                     /gene="yoeB"
                     /locus_tag="ACHURS_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFR1235874.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(25137..25388)
                     /gene="yefM"
                     /locus_tag="ACHURS_000032"
     CDS             complement(25137..25388)
                     /gene="yefM"
                     /locus_tag="ACHURS_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFR1235875.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            25671..25721
                     /gene="hisL"
                     /locus_tag="ACHURS_000033"
     CDS             25671..25721
                     /gene="hisL"
                     /locus_tag="ACHURS_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFR1235876.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    25697..25820
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            25867..26766
                     /gene="hisG"
                     /locus_tag="ACHURS_000034"
     CDS             25867..26766
                     /gene="hisG"
                     /locus_tag="ACHURS_000034"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFR1235877.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            26772..28076
                     /gene="hisD"
                     /locus_tag="ACHURS_000035"
     CDS             26772..28076
                     /gene="hisD"
                     /locus_tag="ACHURS_000035"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFR1235878.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDDALREYSAKFDKTTVNALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVKDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MAHQVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            28073..29143
                     /gene="hisC"
                     /locus_tag="ACHURS_000036"
     CDS             28073..29143
                     /gene="hisC"
                     /locus_tag="ACHURS_000036"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFR1235879.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTQDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLTEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVTQIIAER
                     EYLIAALKEIPCVEQVFDSATNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            29143..30210
                     /gene="hisB"
                     /locus_tag="ACHURS_000037"
     CDS             29143..30210
                     /gene="hisB"
                     /locus_tag="ACHURS_000037"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFR1235880.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREILNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKMALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            30210..30800
                     /gene="hisH"
                     /locus_tag="ACHURS_000038"
     CDS             30210..30800
                     /gene="hisH"
                     /locus_tag="ACHURS_000038"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFR1235881.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            30800..31537
                     /gene="hisA"
                     /locus_tag="ACHURS_000039"
     CDS             30800..31537
                     /gene="hisA"
                     /locus_tag="ACHURS_000039"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFR1235882.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPERVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            31519..32295
                     /gene="hisF"
                     /locus_tag="ACHURS_000040"
     CDS             31519..32295
                     /gene="hisF"
                     /locus_tag="ACHURS_000040"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFR1235883.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDGETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            32289..32900
                     /gene="hisIE"
                     /locus_tag="ACHURS_000041"
     CDS             32289..32900
                     /gene="hisIE"
                     /locus_tag="ACHURS_000041"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFR1235884.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(32997..33974)
                     /gene="wzzB"
                     /locus_tag="ACHURS_000042"
     CDS             complement(32997..33974)
                     /gene="wzzB"
                     /locus_tag="ACHURS_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFR1235885.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVAKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(34120..35286)
                     /gene="ugd"
                     /locus_tag="ACHURS_000043"
     CDS             complement(34120..35286)
                     /gene="ugd"
                     /locus_tag="ACHURS_000043"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFR1235886.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTEAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLAIFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(35536..36942)
                     /gene="gndA"
                     /locus_tag="ACHURS_000044"
     CDS             complement(35536..36942)
                     /gene="gndA"
                     /locus_tag="ACHURS_000044"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFR1235887.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(37106..38476)
                     /gene="cpsG"
                     /locus_tag="ACHURS_000045"
                     /gene_synonym="manB"
     CDS             complement(37106..38476)
                     /gene="cpsG"
                     /locus_tag="ACHURS_000045"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFR1235888.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALARGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEEKTKLI
                     LELLNK"
     gene            complement(38581..40017)
                     /gene="cpsB"
                     /locus_tag="ACHURS_000046"
     CDS             complement(38581..40017)
                     /gene="cpsB"
                     /locus_tag="ACHURS_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFR1235889.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGAMHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(40020..41243)
                     /gene="wcaI"
                     /locus_tag="ACHURS_000047"
     CDS             complement(40020..41243)
                     /gene="wcaI"
                     /locus_tag="ACHURS_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFR1235890.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGSKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLDLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(41240..41719)
                     /gene="gmm"
                     /locus_tag="ACHURS_000048"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(41240..41719)
                     /gene="gmm"
                     /locus_tag="ACHURS_000048"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFR1235891.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELILPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(41722..42687)
                     /gene="fcl"
                     /locus_tag="ACHURS_000049"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(41722..42687)
                     /gene="fcl"
                     /locus_tag="ACHURS_000049"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFR1235892.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            complement(42690..43811)
                     /gene="gmd"
                     /locus_tag="ACHURS_000050"
     CDS             complement(42690..43811)
                     /gene="gmd"
                     /locus_tag="ACHURS_000050"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFR1235893.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(43837..44385)
                     /gene="wcaF"
                     /locus_tag="ACHURS_000051"
     CDS             complement(43837..44385)
                     /gene="wcaF"
                     /locus_tag="ACHURS_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFR1235894.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(44401..45147)
                     /gene="wcaE"
                     /locus_tag="ACHURS_000052"
     CDS             complement(44401..45147)
                     /gene="wcaE"
                     /locus_tag="ACHURS_000052"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFR1235895.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            complement(45158..46375)
                     /gene="wcaD"
                     /locus_tag="ACHURS_000053"
     CDS             complement(45158..46375)
                     /gene="wcaD"
                     /locus_tag="ACHURS_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFR1235896.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVIGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            complement(46350..47567)
                     /gene="wcaC"
                     /locus_tag="ACHURS_000054"
     CDS             complement(46350..47567)
                     /gene="wcaC"
                     /locus_tag="ACHURS_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFR1235897.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVDEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRATYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(47564..48052)
                     /gene="wcaB"
                     /locus_tag="ACHURS_000055"
     CDS             complement(47564..48052)
                     /gene="wcaB"
                     /locus_tag="ACHURS_000055"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFR1235898.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(48055..48894)
                     /gene="wcaA"
                     /locus_tag="ACHURS_000056"
     CDS             complement(48055..48894)
                     /gene="wcaA"
                     /locus_tag="ACHURS_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFR1235899.1"
                     /translation="MKENPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(48987..51149)
                     /gene="wzc"
                     /locus_tag="ACHURS_000057"
     CDS             complement(48987..51149)
                     /gene="wzc"
                     /locus_tag="ACHURS_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFR1235900.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWERLMGRQNETVKVTTFNRPKEMAGQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVSWASKNYDLVLIDTPPILAVTDAAIVGRHAGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(51152..51595)
                     /gene="wzb"
                     /locus_tag="ACHURS_000058"
     CDS             complement(51152..51595)
                     /gene="wzb"
                     /locus_tag="ACHURS_000058"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFR1235901.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(51601..52740)
                     /gene="wza"
                     /locus_tag="ACHURS_000059"
     CDS             complement(51601..52740)
                     /gene="wza"
                     /locus_tag="ACHURS_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFR1235902.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            53049..53198
                     /locus_tag="ACHURS_000060"
     CDS             53049..53198
                     /locus_tag="ACHURS_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1235903.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            53399..54982
                     /locus_tag="ACHURS_000061"
     CDS             53399..54982
                     /locus_tag="ACHURS_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFR1235904.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPRQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIVGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
     gene            complement(55256..57109)
                     /gene="asmA"
                     /locus_tag="ACHURS_000062"
     CDS             complement(55256..57109)
                     /gene="asmA"
                     /locus_tag="ACHURS_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFR1235905.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(57131..57712)
                     /gene="dcd"
                     /locus_tag="ACHURS_000063"
     CDS             complement(57131..57712)
                     /gene="dcd"
                     /locus_tag="ACHURS_000063"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFR1235906.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(57804..58445)
                     /gene="udk"
                     /locus_tag="ACHURS_000064"
     CDS             complement(57804..58445)
                     /gene="udk"
                     /locus_tag="ACHURS_000064"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFR1235907.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            58763..62080
                     /locus_tag="ACHURS_000065"
     CDS             58763..62080
                     /locus_tag="ACHURS_000065"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFR1235908.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     VFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMIAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHEFFQQAAKAVASKGISIALPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPAEAILDHAESVQKLQLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(62190..63038)
                     /gene="alkA"
                     /locus_tag="ACHURS_000066"
     CDS             complement(62190..63038)
                     /gene="alkA"
                     /locus_tag="ACHURS_000066"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFR1235909.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADNYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAGR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKRRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            63172..64524
                     /gene="yegD"
                     /locus_tag="ACHURS_000067"
     CDS             63172..64524
                     /gene="yegD"
                     /locus_tag="ACHURS_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR1235910.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     SVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQEILERAAKRAGFRDVVFQYEPIAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISNELATLISQRGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPQIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(64537..66477)
                     /gene="yegI"
                     /locus_tag="ACHURS_000068"
     CDS             complement(64537..66477)
                     /gene="yegI"
                     /locus_tag="ACHURS_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFR1235911.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVSALDSLRQQLKKCTVSAMHVYPGHLADCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ANCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(66474..67235)
                     /gene="pphC"
                     /locus_tag="ACHURS_000069"
     CDS             complement(66474..67235)
                     /gene="pphC"
                     /locus_tag="ACHURS_000069"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HFR1235912.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVVWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAFMVQKVQDGELGLNDVLATNMVLTIHQRLFAEAEA
                     KALAVRDFACTFLGLISSPDGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTGRAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(67232..67891)
                     /locus_tag="ACHURS_000070"
     CDS             complement(67232..67891)
                     /locus_tag="ACHURS_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFR1235913.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(68112..68171)
                     /gene="ibsA"
                     /locus_tag="ACHURS_000071"
     CDS             complement(68112..68171)
                     /gene="ibsA"
                     /locus_tag="ACHURS_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFR1235914.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(68444..68500)
                     /locus_tag="ACHURS_000072"
     CDS             complement(68444..68500)
                     /locus_tag="ACHURS_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR1235915.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(68771..68827)
                     /locus_tag="ACHURS_000073"
     CDS             complement(68771..68827)
                     /locus_tag="ACHURS_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR1235916.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            69106..70347
                     /gene="mdtA"
                     /locus_tag="ACHURS_000074"
     CDS             69106..70347
                     /gene="mdtA"
                     /locus_tag="ACHURS_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFR1235917.1"
                     /translation="MKGSYKSRWVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQQS
                     PAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTIIAANTVTVRSRVDGQLMALHFQ
                     EGQQVKAGDLLAEIDPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSR
                     QELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSG
                     DTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTLLS
                     LDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGH
                     FVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQ
                     SATTPEEKATSREYAKKGARS"
     gene            70347..73469
                     /gene="mdtB"
                     /locus_tag="ACHURS_000075"
     CDS             70347..73469
                     /gene="mdtB"
                     /locus_tag="ACHURS_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFR1235918.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFTNMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDQGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTEITPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQEIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            73470..76547
                     /gene="mdtC"
                     /locus_tag="ACHURS_000076"
     CDS             73470..76547
                     /gene="mdtC"
                     /locus_tag="ACHURS_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFR1235919.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVSVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRHPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            76548..77963
                     /locus_tag="ACHURS_000077"
     CDS             76548..77963
                     /locus_tag="ACHURS_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1235920.1"
                     /translation="MTELPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLLLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLITLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            77960..79363
                     /gene="baeS"
                     /locus_tag="ACHURS_000078"
     CDS             77960..79363
                     /gene="baeS"
                     /locus_tag="ACHURS_000078"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFR1235921.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            79360..80082
                     /gene="baeR"
                     /locus_tag="ACHURS_000079"
     CDS             79360..80082
                     /gene="baeR"
                     /locus_tag="ACHURS_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFR1235922.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
BASE COUNT        19323 a        21222 c        20482 g        19138 t
ORIGIN      
        1 cgattaagtc agccattctt tttcctcgct tttttttata gtcatatcgt caaaaaaatc
       61 caggcgaggt ctttatgcaa caagaagcgt taggaatggt agaaaccaaa ggcttaactg
      121 ccgccataga ggccgcagac gccatggtga agtcggcaaa tgttttgctg gtcggctacg
      181 aaaaaatagg ttccgggctg gtgaccgtca tcgttcgtgg tgatgtcggg gcggtaaaag
      241 cggcaacaga cgcgggtgcc gcagctgcgc ataacgtcgg cgaagtaaaa gccgtacacg
      301 ttattccccg cccacatacc gatgtcgaaa aaatcttacc aaagggaatt aacccatgag
      361 cagcaatgaa ctggtcgaac agatcatggc gcaggtcatc gctcgcgtag cgacgccaga
      421 gcatgaggtt ctcccgaaaa ccactcatca aatacgatag acggctatgg cagaaaaaag
      481 ctgcacgcta acagaatttg ttggcaccgc tattggcgac actgtaggtc tggtcattgc
      541 gaacgttgat agcgccctgt tggacgcgat gaaacttgaa aaacgctatc gttccattgg
      601 cattctgggt gcgcgtattg gcgcagggcc acacattatg gccgctgatg aagcggtaaa
      661 agccaccaac actgaagtta ttagcattga acttcctcgc gatactaaag gcggtgccgg
      721 acacggctca ctgatcctct taggtggcaa cgatgtttcc gacgtgaaac gcggtattga
      781 agtcgccctg aaagaactcg atcggacctt tggcgatgtc tatgccaacg aagccggaca
      841 tcttgaactg caatataccg cccgcgccag ctatgcactg gaaaaagcgt ttggtgcacc
      901 actgggacgg gcttgcggcg ttattgtcgg cgctccggct gctgtgggtg ttctgatggc
      961 cgacaccgcg ctgaagtccg ccaatgtgga tgttgtggca tacagctccc cagctcaagg
     1021 caccagctac agcaatgaag tcattctgat tatctcgggt gactccggcg cagtccgcca
     1081 ggcggtcatc tccgcacgcg aaatcggtaa aaccgtgctt tcgactctcg gcgatacacc
     1141 gaaaaacgat cacccttcct acatctgaat tctctgaggc agatttatga gatcgaaaag
     1201 atttgaagca ctggcaaaac gcccggtcaa ccaggacggt ttcgttaaag agtggattga
     1261 agaaggcttt attgcgatgg aaagcccgaa cgatcctaaa ccgtcaatca aaattgttaa
     1321 cggcgtcgtt accgaactgg atggaaaacc cgccagccag tttgacctga tcgaccactt
     1381 tatcgcccgc tacggcatca atctcgcgcg cgccgaagaa gtcatcgcga tggattcggt
     1441 aaaacttgcc aatatgttgt gcgatccaaa tgtcaaacgt agcgatatcg ttccccttac
     1501 cacagcaatg acgcctgcaa aaatcgtcga agtggtttcg cagatgaacg tggtagagat
     1561 gatgatggcg atgcagaaaa tgcgcgcccg tcgaacacca tcacaacagg ctcacgtcac
     1621 caacgtcaaa gataacccag tacaaattgc cgccgacgca gctgaaggcg catggcgcgg
     1681 gtttgatgaa caggaaacca ccgttgccgt tgctcgctat gcaccgttca acgccatcgc
     1741 cctgctggtc ggctcacagg tgggacgtcc gggcgtcttg acccagtgtt cactggaaga
     1801 agccaccgaa ctgaaactcg gtatgttagg ccacacctgt tacgccgaaa caatttctgt
     1861 ctatggtaca gaacctgttt tcaccgatgg tgatgatacc ccgtggtcaa aagggtttct
     1921 cgcgtcatcc tatgcttctc gtggcttaaa aatgcgcttt acttccggct ccggctctga
     1981 agtacagatg ggttacgcgg aaggcaaatc aatgctttac ctcgaggctc gttgcattta
     2041 catcaccaaa gccgcaggcg ttcagggtct gcagaatggt tcggtaagct gcatcggtgt
     2101 tccatcagct gtgccatcag gcatccgcgc agtattagcg gaaaacttaa tttgctcatc
     2161 gctggatctg gaatgcgcct ccagtaatga ccagaccttt acccactcgg atatgcgccg
     2221 taccgcacgt ttcctgatgc aatttctgcc aggcaccgac tttatctctt ccggtttttc
     2281 cgctgtgccg aactacgaca acatgtttgc tggttcaaac gaagatgctg aagacttcga
     2341 tgattacaac gtcattcagc gtgacctgaa ggttgatggt gggctgcgac cagtacgcga
     2401 agaagatgtt atcgctatcc gcaataaagc cgctcgcgct ttacaggctg tctttgccgg
     2461 tatgggacta ccgcctatta ccgatgaaga agtcgaagcg gcaacctatg cccacggttc
     2521 aaaagatatg cctgagcgca atattgtgga agacatcaag ttcgcccagg agattatcaa
     2581 taaaaaccgg aacagcctgg aagtggtgaa agccttggcg caaggcggtt ttaccgatgt
     2641 cgcccaggac atgctcaaca tgcaaaaagc aaaactgacc ggcgattatc tccatacctc
     2701 agccatcatc gtcgatgacg gacaagtgct ctctgcggtc aatgacgtca atgattacgc
     2761 cggaccggct acaggttacc gcctgcaagg ggaacgctgg gaagagatca aaaatattcc
     2821 cggtgcactt gatcccaacg aaattgacta agggtgagaa atggaaatta acgaaaaatt
     2881 actgcgtcag attatcgaag acgtgctggc agaaatgcaa cccagcgaca agtccgtttc
     2941 atttcgcgca cctgtctctg caactgtccc ttccgcgcca gataccggaa attttctgac
     3001 cgaaattggc gaagcccaac aaggcactca gcaagatgaa gtcattattg ctgtaggccc
     3061 ggcgttcggc ctggcgcaaa ccgtcaatat catcggtatt ccgcataaaa acatcttgcg
     3121 cgaagtgatt gccggtatcg aagaagaagg cattaaagcc cgtgtaattc gctgctttaa
     3181 atcgtccgat gtcgcattcg tagcagtcga aggcgatcgt cttagtggtt caggtattgc
     3241 tatcggtatt cagtcgaaag gcacaacggt tatccaccag cagggacttc ctcccttgtc
     3301 taacctggaa cttttcccac aagcgccttt gttgacgctg gatacttatc gtcagattgg
     3361 caaaaacgcc gcgcgttatg cgaagcgcga gtctccacaa ccggtgccga ccctcaacga
     3421 tcaaatggcg cggccgaaat accaggccaa atctgccatt ttgcatatta aagagacgaa
     3481 gtacgtggtc actggcaaaa aaccgcaaga actgcgcgtg acgttttaac aaaggaaacc
     3541 ttcatgaata ccgatgcaat tgagtcgatg gtcagggacg tgttgaaccg catgaacagt
     3601 ttacaggatg cagctcctgt ttctgcggtc cccaacgcat caatactgag cgccaaagtg
     3661 acggattacc cgctggcaaa taaacatccg gaatgggtta aaacagcgac caataaaacg
     3721 ctcgacgatt tcactctgga aaatgtgcta agcgataacg tcaccgccct ggatatgcgc
     3781 attacccctg aaacattgcg tattcaggct gcgattgcca gagatgctgg tcgcgatcgt
     3841 ctggcgatga attttgaacg cgccgctgag cttacctcag taccagatga tcgcatcctc
     3901 gaaatctaca atgcgttacg cccttatcgt tcaactaagc aagaacttat tgccattgct
     3961 gacgatcttg aacagcgtta tcaggccaaa atttgtgctg catttgtccg tgaagctgcg
     4021 gaactttatg tcgagcgcaa aaaactgaaa ggcgacgatt aaggacgaac tatgcgatat
     4081 atagcaggca ttgatatcgg caactcatca acagaggttg cgctggcaac gctcagtgca
     4141 actggagagt tgtcgttcgt gagtagcgcc ctggaggaaa ccaccggaat caaaggtacg
     4201 ttgcgtaacg tccatggtat tcaggaggcg ctcgcacagg caacgaaaaa agtcggcatc
     4261 aatgttagcg atatttcgct tattcgtatc aacgaagcaa ctccggtcat tggtgatgtg
     4321 gcgatggaaa ccatcactga aactatcatc accgaatcaa ccatgatcgg tcacaacccg
     4381 aaaactccgg gcggagtggg gctgggcgta gggttcacca tcactccgca agagttatta
     4441 acacgcccgg cggacacacc ttacatcctg gtggtgtcat cggcatttga ttttgccgat
     4501 atcgcgacga tgataaacgc ctccgtacgc gccggatatc agctgactgg cgttattctg
     4561 caacgcgacg atggcgtact ggtcaataac cgcctggaaa ttcctttacc tattgtggat
     4621 gaagtcctct acatcgatcg tattccgctc ggcatgctgg cagcaattga agttgccgtt
     4681 cccgggaaag ttatcgaaac cctgtctaac ccgtatggga ttgccaccgt attcgccctc
     4741 aatgctgaag agactaaaaa tattgttcct gtcgcgcggg cgctaatcgg caaccgttca
     4801 gcagtggtag tgaaaacgcc ctctggcgac gttaaagccc gttcgatacc cgccggaaat
     4861 atcgaactgc tttctgccgg gcgcacaacg cgcgttgatg tagctgctgg tgccgatgcc
     4921 attatgaaag cggtcggtga gtgcccaaaa ctggaaaacg tgacgggaga accaggcacc
     4981 aacatcggcg gaatgctgga acatgttcgc cagaccatgg cggaactgac caacaaaccc
     5041 agcaacgaaa tttttattca ggatttgctg gcgatagata cctctgtccc cgtcagcgta
     5101 accggtggtc tggcaggtga attttcactg gaacaggctg tgggtattgc ctctatggtg
     5161 aaatccgacc gtctgcaaat ggcgatgatc gccagtgaaa ttaagcaaaa actgcatgtt
     5221 gatgttcagg tcggaggcgc agaagcagaa gccgccattc agggggcctt gactacccct
     5281 ggaaccacac gtccactggc aattctggac ttaggtgcag gctctaccga tgcctccatc
     5341 atcaatcaga gtggggaaat cgttgccact caccttgcgg gagcgggcga catggtaacg
     5401 atgattatcg ctcgtgagct gggcttaaat gatcgttatc tggcagaaga aatcaaaaag
     5461 tacccgttag caaaagtaga aagcctgttc cacttacgcc atgaagacgg tagcgttcag
     5521 ttcttcccta ctcccctatc cccccatgta tttgcccgcg tctgtgtagt gaaacccgac
     5581 gaactggtgc ctattcccgg tgatttgacg ctggaaaaag tacgcgctgt tcgtcgcagt
     5641 gcgaaagagc gtgtcttcgt aaccaatgcc ctgcgcgcgc tacgtcaggt aagccctgcc
     5701 ggaaatattc gcgacattcc gttcgtcgtt ctggtgggtg gttcttcgct ggatttcgaa
     5761 gtaccgcaat tggtcaccga cgcgctggca cattatcgac tggtcgccgg gcgggggaat
     5821 atccgtggta gcgaaggtcc gcgcaacgcg gtagctactg ggctactcat cgcctggcac
     5881 aaggagtcga ttcatggaaa gtaaagagcg tgcaccagcc atcgttgtca tggagatcgg
     5941 tgactgtatc actacatggg atgaggtgct tcttggcatt gaagaggaag gtattccctt
     6001 tcgcattcaa cacattccgt caggtgaagt tatcgatagc gcctggctgg ctgctcgtca
     6061 atctcccctg ttggttggca ttgcctgcga ccgggaagaa ctgattgtgc attacaaaaa
     6121 cttaccggca tcagcgccac tgtttacgct gacgtatcaa caagataacc atacccggcg
     6181 cagcatcggc actaacgctg cccgcctggt aaaaggaatt cccttccggg aattccactc
     6241 ataatccaca ggagaaacgc tgtatgaaca acgcactagg actggttgaa acaaaagggt
     6301 tagttggcgc aattgaagct gcagatgcca tggttaaatc cgccaacgtg cagctggttg
     6361 gctatgaaaa gattggttcg ggtctgatta cggtgatggt ccgcggtgat gttggcgcag
     6421 taaaggccgc cgttgatgcg gggagcgctg cggcaagcgc cgttggcgaa gtgaaatcct
     6481 gccatgtcat cccgcgccca cacagtgatg ttgaggctat tttacctaaa tcggcataac
     6541 cccatttgct aaggagcaca gcgtgaagca atcactggga ttacttgaag ccagtggtct
     6601 ggctctcgcc atcagttgcg cagacgctat ggcaaaagcc gcgtccatca cgctggttgg
     6661 ccttgagaag accaatggct caggctggac gctcatcaaa atcaccggtg acgtcgcctc
     6721 ggttaatgcg gcgatcgcga cgggagccag tttcgccgga cagcgaaatg gccttgtcgc
     6781 acacaaagtg attgcccgcc cgggagacgg cattctgccc cacacaactg aagcattgac
     6841 cgtgacggtt gaaacaagca aaacaccagc aacaaccgtc acctcagaga ttatcagttg
     6901 taatttgtgt cttgacccgg catgtcagcg gcaaaaaggt gagccccgct cactttgcct
     6961 gtacgccaat aagcgagggg agtagcagtg gataaacaaa ccttgcagga aacagtgagc
     7021 cagattctgg atgagatgcg cgagcgccct atcccactgg gcgtttccag tcgccatata
     7081 catctttcat cagcggatta tttgcgtctt tttcctgaac aaccgattag cgagaaaaag
     7141 ccgttaatgc aacctggcca atacgctgct gaacaggttg ttacgctgga gggaccaaaa
     7201 ggcaaattgc acaacgttcg cttgttgggg ccgctacgca atgcaagcca ggtggaaatt
     7261 tcccggacgg acgccagaac gttagggatc accgccccgc tacgcatgtc aggcgatctc
     7321 agcggcactc cggggattcg tttgataagc ccatttgcgg aactggagat cacatctggc
     7381 gtcattgtcg cgcaacggca tattcatatg tctccgctgg atgccctgat ttatggcgtt
     7441 acgcatggcg ataaagtggc ggttgccatt gaaggtgatg agcgacgact gatattcgaa
     7501 aacgtcgctg ttcgcgtctc tccggatatg cgtcttgaaa tgcatattga taccgatgag
     7561 gccaacgccg caggcgctga tagccctgac gtttttgcca cactggtgtc agtgccatga
     7621 ataacatgct gatgcaacgt attgttgacg aagtggtttt tcgccttaaa caacgtgcgg
     7681 gaaaaacact cgtgctgact gtttttcaat tgcgagacgc cagcgttcaa gagagcgttc
     7741 atcagtatgc gtcattacaa atccgatatg tcgatctgcc gctgctgcga caacttgcgg
     7801 agaacgaaac gtcagatcga gcatctatcc agatacacga agcgttagcc tggggaatgc
     7861 acattcaatt gtcgttgcag cgtcatttcc tgaacgccat cgaattgaag actctcgccc
     7921 gtctaccgct aagctggtgc gacgaacaag ggcagccgat ctaccttcat cataatcgac
     7981 ttttaagtta tgccgatatc gcacagttaa gctcgggaat actggtgtta cagcggaaat
     8041 gttgtgtgac cgcactggct cgtgaagcgg caattacccg aaatattcag ttaatcaggc
     8101 aggagtaacc tatgcatctg gctcgagtca ctggcgcggt agtatccacg caaaaatcac
     8161 cttctctgat cgggaaaaaa ctgctgctgg ttcgccgggt cagtgctgac ggcgaactgc
     8221 ctctcacgcc attaaccggc gacgaagttg ccgttgattc agtcggcgca ggcgttggtg
     8281 agttggtact cctcagcagt ggttccagcg ccagacatgt gttttcaggc cctaacgagg
     8341 ccatcgacct ggcagtggtc ggcattgtcg atacgctttc gcgctaagga gctaaaagat
     8401 ggcaatttat acccgaacag gtgattcagg aagtacgtct ttatttaccg gacagcgcgt
     8461 cagcaaaact catctacgtg tcgaaaccta tggcacgctg gatgaactga acgcgacact
     8521 cagtctgtgt tattgcgcca ccgccatcga aagccatcgc atcttactgg aagccattca
     8581 acaacagata ttttggttta gcgccgaact cgccagtgaa agcgaacagc cgtcagcgca
     8641 acagcgttac atcggtacgg aagaaattgc cgcgttggaa aacgcgatcg atagtgcaat
     8701 gaacgccgtc cccccagttc acagcttcat tctgcctggt cgatgtgaag ccgcaagccg
     8761 catgcatttc gcccgcacgg tggctcgacg ggctgaaaga cggctggttg aactgacaac
     8821 ggaaaccacc gtccggaatg ttttgctgca ctatatcaac cgactttccg attgcctgta
     8881 tgccctcgcc cgcgtggaag acaacgttgc tcatcaaaac ctgatgattc aggaaatcac
     8941 aaaacgttat cacgaagcca accacacacc agcattgaag gaacgcacaa tgccgctcac
     9001 ttttcaggat cttcaccagc tcattcgtag tgcggcaatg cgcgcggatg aactgcatat
     9061 tcccgttgtc atcagcattg ttgatgccaa cggaacagaa agtgttacct ggcggatgcc
     9121 tgatgccctg ttggtcagca gtgaactggc accgaaaaaa gcctggaccg ccgtggcgat
     9181 gaaaacggca acccacaagc ttgctgatac cgtgcaaccc gacgcgccgc tttacgggct
     9241 cgaaagccat atgcaaggaa aagtcgtcac ttttggtggc ggcttccccc tctggcgtga
     9301 cggaaaattg cttggcgggc ttggcatcag cggcggtagc gttgaacaag acatggatat
     9361 tgcccaaagc gcaatggcgg caattaacgt gggagtaaac caatgaataa ctctgaatta
     9421 gaaacactga tccgcaatat tctgcgtgag caacttgtcc ccgctacacc acgaacgcaa
     9481 agaaacgcta ttttccagac tgtcgatgaa gcggtctgcg cagcacacta ggccttttta
     9541 cgctttcaac agtgcccgct taaaacccgt agcgccatta tcagcgccat tcgcgaagaa
     9601 ttaatgcccc gccttaccga actggcagaa gaaagcgcca aagaaacggg gatgggaaat
     9661 aaagaagata agtacctaaa aaacaaagcg gcactcgata acacgccagg aattgaagat
     9721 ttaaccacca cagcactaac cggtgatggt ggcatggttt tgtttgagta ctctccgttt
     9781 ggggtgattg gctcggtcac gccaagcacc aaccccacgg aaaccatcat taacaacagc
     9841 atcagcatgc tggcagcagg taatagcgtc tacttcagtc cacacccggg tgccaaaaac
     9901 gtttcgttaa aactgattac cacaatcgaa gacatcgcgt ttcgctgtag tggcattcat
     9961 aacttgatcg ttaccgtatc tgaaccgacc tttgctgcca ccgaacagat gatggcgcac
    10021 cctaaaatca gcgttctggc gattaccggc ggtccgggga tcgtcgccat gggaatgaaa
    10081 agtggcaaga aagtgattgg tgccggagcg ggtaaccctc cgtgcatcgt cgatgaaacg
    10141 gcggatattg tgaaagccgc cgaggacatc atcaacggcg cttcatttga ttacaacctg
    10201 ccgtgtattg ctgaaaaaag cctgattgtc gtggcatcag tggcagatta tttaattcag
    10261 cagatgcaaa gttttggcgc acttcttctt aactatgagc aaactgagaa attgcgtgca
    10321 atttgcctgc cagacggtag cgccaacaaa aaactggttg ggaaaagccc atcagccttg
    10381 ctggaagcgg caggcctccc tttgcccgca aaagcgccgc gcctactgat tgctgttgtc
    10441 gacgccgatg atagttgggt tacttgtgag caactaatgc caatgctgcc cattgtgaag
    10501 gtcaaggatt ttgacagcgc tctaaccctg gcgctgaaag tggaagacgg cttgcaccac
    10561 acggcaatta tgcactctca gaacgtctca cgactcaacc ttgccgcgcg cgttatgcaa
    10621 acctcaatat ttgtcaaaaa cggcccctcc tgggcgggta ttggcgtcgg cggcgaaggc
    10681 tttacgacct ttaccattgc aacaccgact ggagagggta caacgtcagc acggactttt
    10741 gcccgctcac gacgctgcgt gttaaccagc ggcttttcaa ttcgctagag aaacggaaat
    10801 gaatactttt tcactgcaga cgcgtttata cagcggacca ggaagtctca aggtacttag
    10861 ccgtttctct catagacaca tctggattat ttgcgatgcc tttctggcac gctcaccact
    10921 tattgacacg ttgcatcagg cgctgcccga cagtaatcgt ctcagtattt ttagcgaaat
    10981 aacgcccgcc ccaactatcc agaccgtggt gaaaggcatt gcgcaaatgc agactttacg
    11041 cccggatgta gttatcggtt ttggcggcgg ttctgccctg gacgccgcaa aagcaattgt
    11101 ctggttcggt cgccagtgtg gcattgaaat tgaaacctgc gtagccattc ctaccaccag
    11161 cggtatcggt tccgaagtga ccagtgcttg tgtcatcagc gatcctgaga aagggattaa
    11221 atatcccctt ttcgataatg cactttatcc ggatatcgcg atccttgatc catctttggt
    11281 cgtcagcgtg ccaccggcga taacggccaa taccgggatg gatgttttaa cccatgcgct
    11341 tgaagcttat gtttctcctc gcgccagcga cttcacggat gcactggtcg aaaaagccgt
    11401 gcaaattgtt tttcaatacc tgcccactgc agtgaaaaaa ggcgattgtc ttgccacccg
    11461 tggcaaaatg cataatgcat cgacgcttgc aggaatggcc tttagccagg ccggactcgg
    11521 tttaaaccat gcaattgctc atcaactcgg cggacagttt catctgccgc atggactggc
    11581 gaatgcgctc ctgctcacgg cagttatccg ctttaatgct ggcgatccgc gcacagccaa
    11641 acgctatgcc cggctggcga aaacgtgcca tttatgcccg gacaacgcca atgacaccgc
    11701 cagcctgaat gcacttattc aacatattga gcagcttaat acaacctgta cgctgccgac
    11761 tcttgccaat gcgctgaaag aaaaaaaagc agaatggtca atacgcatac cagatatggt
    11821 tcaggcggcg ctggcggatg caacgttgcg taccaacccg cgtgcagctg atgcctccgc
    11881 gattgcagaa ctgctcgagg agttgttatg aacgtcggat taatctcctg cgacgaccag
    11941 accatccgcg agcgagtaca agcagcaggt gtcgtgggtg ctggcggcgc aggttttcca
    12001 acccatatta agctacaagc gcgtgctgac actttcctgg tgaatgcggc ggaatgcgaa
    12061 cccatgttga aagtagatca acagttaatg gcgcagcaag ccagccgcct gcttcgcggt
    12121 gttcactatg cgatgaaagc caccggggcc agtagcggaa tcatcgcgct gaaagaaaaa
    12181 tatcaacggg caataaacgc actcacacca ctgttaccac cggatatccg cctgcatatt
    12241 ttgcccgacg tttaccccgc aggagacgaa gtgctgacca tctggatggc aactggtcgc
    12301 cgcgttccac ccgctgcatt acccgttagc gtcggtgtgg tggtcaataa tgttcaaacc
    12361 gtacttaata ttgccagagc cgtcgagcaa cagtatccag tgacgcgccg cacactcacc
    12421 gttaatggtg cagtggcgaa gccaataacg gtaaccgtcc ccatcggcat gtctttacgt
    12481 gaagtcctgg cgctggcggg cggcgcaaca gtggatgctc ctggttttat caatggcggg
    12541 ccgatgatgg gaagtttgat cacatcgctc gacacaccag tcagcaaaac cactggcggc
    12601 ttgctggttt tacccaactc tcattcactg atccaacgtc gaatgcagaa tgaccgcagc
    12661 gtacttgccg tcgcgaaaac tgtttgtgag caatgccgct tgtgtaccga tctctgccca
    12721 cgtcatttaa tcggtcacga actggcaccc catttattag tacgtgcggt taactaccaa
    12781 cagctcgcca cgccgcaact gttactgaca gcgctcacct gttcagagtg caacgtctgc
    12841 gccagtgtcg cctgtccggt aggaatttca ccaatgcgca taaaccgcct gcttaagcag
    12901 gaattacgcg cacaaaatct ccgctacgaa ggtgccttaa atcctgcaga tcccatggca
    12961 aattatcgac tgattcccgt aaaacggctg gtaaccagac ttggcttaac cccgtggtat
    13021 caggatgcgc cattgtctga acaggtgcct cagcccgaaa aagtgacgtt attgttacgc
    13081 cagcacatcg gtgccagcgc aattgcgtgt gtacaaaaag gcgatcgcgt cgttcatggg
    13141 caatgtgtag gtcaaattcc acacggcaca ttaggtgccc ccattcatgc cagtattgat
    13201 ggcatggtca gcgatgtgac agaaaacgcg attacgcttg tgagaggtta accatgtcga
    13261 aagcggtagg tattttagaa cttagcagta tcgcgaaagg gatggaaacc ggcgacgcca
    13321 tgctgaaaag cgccaacgtt gagttactgg tcagcaaaac gctatgcccc ggaaaatttc
    13381 tgctgatgct cggtggcgat gtgggcgcgg tgcaacaggc catcaacagc ggagcgtccc
    13441 ttgctggtga aatgctggtc gacagtcttg tcctgccgaa tattcatccc agcctccttt
    13501 ctgcaatcag cggacttaac catgttgaac agcaccgggc tgtcggcgta gtcgaaacct
    13561 ggagtgtcgc cgcctgcatt gacgctgccg attgtgctgt caaagctgca aatgtcattc
    13621 ttgttcgcgt acatatggct tttggcattg gcggtaaatg ctacatggtg gttgctggcg
    13681 atatttcaga tgttaataac gcggtctctg ccgccagcgt gcgcgcgggg gaaaaaggtt
    13741 tgttggtcta tcggtcggtg attgcccgtc cacatgaagc catgtggcgt cagatggtgg
    13801 agggataatg acaactcagc acacaactga acggatgatt caggaatatg tcccaggtaa
    13861 gcaggtcacg ctggcacatg tcatcgccaa tccagggaag gatctgttta agaaattggg
    13921 tttaccagac accatttccg ctattggtat tctgacaatt acccccagcg aagcatctat
    13981 catcgcatgc gatatcgcca caaaatcagg cgctgtagag atcggttttc ttgatcgttt
    14041 taccggtgcc gtagtgctga ctggtgatgt ttctgcgatc gaatatgcac ttagccaggt
    14101 tacccgtacg ctgggtgaaa tgatgcgctt taccgcctgc cccattacgc ggacctgacg
    14161 ttatgcaacg tatgatgctc atcggcccca gccagtgcgg aaaaacgtca ctcacgcaat
    14221 gtctgcgtgg cgaaaatatt cattatcaga aaacgcaggc aatcgtctgg tccccgacca
    14281 ccattgatac accaggcgaa tacctcgaaa accgcagcct ctacagtgca ctgttggtca
    14341 gtgcctgcga agcggacatt atcgcgctgg tgcttaatgc ggacgcaccg tggtcacctt
    14401 tttcgccagg ctttacctcg tcaatgaatc gcccggtcat tggagtagta accaaagcag
    14461 atttagccaa catggaacaa atatctttgg tgaaatgttg gttacgggaa gctggagcgc
    14521 acaacgtgtt agtaaccagt gcggttaata accatggagt tgcagaactt ttagccttgc
    14581 tgcatacaga agaaggctgt cgctaatttc gacaggttat cagatgggaa tgatgacggt
    14641 gatacacaga aaagaatcaa tgagcaatga gggttgccgg gcaaccctca ttgaataaaa
    14701 caggaattac ttcgcttcgc cgtttttcat ttcgcccata gctttcagct ttttggagat
    14761 atcgcggcgt tctttggaga gctcggcatt tttgatgatg taatcgtcaa cgcgatcttc
    14821 atagtcacct ttcatgctgg cgatgatgcc ctggattgct tcaacgctca tcccaggctt
    14881 gatgtaatcg ctcaggttgt ccagcagcag gacgcgcttc tggttgtcac ggatcttttt
    14941 ctcaacgtcc tgaatttcac gttgcagttt gttcttacga cggaacagac gaacaaattc
    15001 cagtacgtcc tggaatgaag gcttggtagt ttccattttt atacccctga taatgtgaga
    15061 gtcagattcg tttaagcaac cgctattcgt tagggccaac attactcata gctgccgcag
    15121 atgacaatgc ttttatccct tttcactatc atacccttta tccttgctga atcgaagcag
    15181 cagcaagatg attctgaagt tcaggaatta tttgcgcaag gcccggcaaa tcagattcga
    15241 tagcaactca agctgatgcg ccgtttccat gttcagccac acataaccat agattggtgt
    15301 ttccactacc ttcaggtcat ggtggttatt gagcaactga cgcagctctt ccacagcatc
    15361 gttcaatttt tccgtattgg caaaaaccgg ctgcggatta ccttcgtaca gcgcatgaac
    15421 aaagctcagc agtatttgct gcatcatgtg ctgggtatca cgaagttttt gcgcgttcaa
    15481 taacacgaaa tggctggggc gcgtggccca gtacgcattg atttgcaact ccagcataca
    15541 aaccagattg cggttaatgg tctggatacc ttcatatatc gattttggaa tacgggtttc
    15601 tttgctggcg ggcgcaatca gcccacgcat tttcacggca tcggtcagga gtttttgtag
    15661 atggctttcc agacgtgggc gttcgagtaa gttcggtgag aatgcagatt gatagacccg
    15721 attatactcg gtcagacttt tcgccagttg aatgcgccag tggatgaacg cccgttgtgg
    15781 ccagataccg gtaaacaaca ttgccagtaa agagccgagg atcacatcgc cgcttcgcca
    15841 taacgccgta tcaatttcac ctgtcgggga accaacaaca attgccagcg tcaccccaat
    15901 caataatcct tgatacggtt tcttgcccag cgccagccaa ccgcaaagaa acatcgccgc
    15961 cgcgcaccag actaacatca gcggtaacga aattaactcc agttgcagag cgataagacc
    16021 taaaatcgaa cccaacaccg taccgccaat acgttcgaag gcacgaggga caacgttacc
    16081 ccagaacgag attggcccca taatcaccac catggtgacc agcggccagg tgctttccgg
    16141 gatagtaaac aggcggataa tgagaaaagt gagcaggaat gccagcgcga cccgagtacc
    16201 atgcactatg cggtaatggc ggtatacccg gatttcaaac gggcttaatg acttatcggc
    16261 acgcacgcag aaactccgta atgaagatta aaagcgctca taactcatct gtcggccaga
    16321 aggcgcgcct ctgaattatc cgacggacaa accgggaaca gtgtaatttc ccggcaggag
    16381 cgaaaaaatt gaaaggcgca tcttactctt ttcgctttca aaaaaagagt ggtcatcgcg
    16441 ttaacacacc gccctgagat gaattagtga tgttttggct gcaccgcaac atacatttcg
    16501 atatcccagt acccatcttc cgcgccattg ttcaaataaa cctcgaagca gggctttggt
    16561 aacatttcat aagcactgtc ctgcaagagg ctattaaaga actgatacca gggtttagca
    16621 aaatcatcac cgactacacg agccaccgcc accgcatact gaccacctga aatttctgtc
    16681 agaatgacgc cctcactgtt ttcgggaagc gtaaagttat tcggtaccgt cacaacggtg
    16741 tcgcagcgta atttttcggc gggtgtttca tctggattgt cgtaatagac agcaacccac
    16801 tccttcggca caatattttt gttatctacc cacatcatca actgctcaaa gcctttcttt
    16861 accgtctgtt cccacgggcc aacgagatgg aaacctgcaa cggtacgttt ctcttcctgc
    16921 gtaatctcgt agttcatgac gcctccatta acactgtttt tatatacagc atagttgcaa
    16981 tattaaaacc acaaggaatg agtgttgatt atgcgagcag actcgcactc ctgccagtct
    17041 gctgcaaaag aaaggtcagg ccttatggtg gaaataatca ctcaggcggg aaaacatgcc
    17101 gccttccccg acagattcca gggtaaccag cggccagtgc gccacctgtt tatcacggtc
    17161 gtaaagttca atttccccta cccgctgatg ggcgctaatt ggcgcggtga gctctttacc
    17221 atcaagggta tatttggctt tgatatgtgg aatttcggct ttcggtagca ccatccagaa
    17281 ctcttgttcc gttcctaggt cgatattttc tttatcgcca taccagatgc gttccgttcc
    17341 gacctttttc ccacggtgca aaatttgcac cgtagtaaag ttttgttgcc cccaacgcag
    17401 taattttctt gcctcttcct cacgaccttt tgcactgtca gcccccatta ccactgcaat
    17461 gagacgacgc tgcccatcta cagccgaagc aatgagatta aacccggcac cagaagtatg
    17521 acccgttttc aggccgtcaa cattcatggt tttatcccac aacaacccgt tacggttttg
    17581 ctgggtgata ccgttccagg tgagactttt ctcactgtac atatgataaa actcgggctc
    17641 gccgtggatg atagcgcgag aaagcacagc taaatcataa gccgagctat gctggccagg
    17701 tgcatccaga ccatgcactg tttcaaaatg cgtatccttg agatgcagct tctcggcata
    17761 gttgttcatc atttcaacaa actgccgttg cccaccggca atatagtcag ccagagcaac
    17821 acaagcgtca tttccggaat ccacaattaa accacggctt aaatcacgta ccgatacgcg
    17881 atcgccctct ttcaaaaaca tcagtgaaga accgacaaac accggattat ctttcgccca
    17941 cgcatcgcgc cccacggtga caatatcgtc tggcgtaatg cgatgactat cgatagcgcg
    18001 atccacgaca taacccgtca tcagctttgt caggctggcg ggattgcgct gttgatgctc
    18061 attacccgcg gtgaggatct gaccggtggt gtaatccatc aatacccagg acccggcatg
    18121 aatctctgga ggctgaggtg aaaaaggaat gttttccgcc gcaaaaccag acgataagtt
    18181 aaaaacgaac aaagaagcag caataataag acggcgtttc aacagcaaac cctcaggagt
    18241 ttcaaatagc tgttcttttt acggaaatac ttatgaactg gctggaataa agtgcaagaa
    18301 aatgtgacta ccctctcatt tttatctgac atgatctgtt gccactcgct gccaaattgt
    18361 ggcgctaaag ctgattagca cggtgatatt tgatactctg gcagacagca gaaataacgg
    18421 atttaaccta atgatgaatg acggtaagca acaatctacc tttttgtttc acgattacga
    18481 aacctttggc acgcaccccg cgttagatcg ccctgcacag ttcgcagcca ttcgcaccga
    18541 tagcgaattc aatgtcatcg gcgaacccga agtcttttac tgcaagcccg ctgatgacta
    18601 tttaccccag ccaggagccg tattaattac cggtattacc ccgcaggaag cacgggcgaa
    18661 aggagaaaac gaagccgcgt ttgccgcccg tattcactcg ctttttaccg taccgaagac
    18721 ctgtattctg ggctacaaca atgtgcgttt cgacgacgaa gtcacacgca acgtttttta
    18781 tcgtaatttc tacgatcctt acgcctggag ctggcagcat gataactcgc gctgggattt
    18841 gctggatgtt atgcgtgcct gttatgccct gcgcccggaa ggaataaact ggcctgaaaa
    18901 tgatgacggt ctaccgagct ttcgccttga gcatttaacc aaagcgaatg gaattgaaca
    18961 tagcaacgcc cacgatgcga tggctgatgt gtacgccact attgcgatgg cgaaactggt
    19021 aaaaacgcgt cagccacgtc tgtttgatta tctctttacc catcgtaata aacacaaact
    19081 gatggcgttg attgatgttc cgcagatgaa acccctggtt cacgtttccg gaatgtttgg
    19141 ggcatggcgc ggcaatacca gctgggtggc accgctggcg tggcatccag aaaatcgcaa
    19201 tgccgtaatt atggtggatt tggcaggaga catttcgcca ttactggaac tggatagcga
    19261 cacattgcgc gagcgtttat atactgcaaa agccgatctt ggcgataacg ccgccgttcc
    19321 ggttaagctg gtgcatatca ataaatgtcc ggtgctggcc caggcgaata cgctacgccc
    19381 ggaagatgcc gaccgactgg gaattaatcg tcagcattgc ctcgataacc tgaaaattct
    19441 gcgtgaaaat ccgcaagtgc gcgaaaaagt ggtggcgata ttcgcggaag ccgaaccgtt
    19501 tacgccttca gataacgtgg atgcacagct ttataacggc tttttcagtg acgcagatcg
    19561 tgcagcaatg aaaattgtgc tggaaaccga gccgcgtaat ttaccggcac tggatatcac
    19621 ttttgtcgat aaacggattg aaaagctgtt gttcaattat cgggcacgca acttcccggg
    19681 gacgctggat tatgccgagc agcaacgctg gctggagcac cgccgccagg tcttcacgcc
    19741 cgagttttta caaggttatg cagatgaatt gcagatgctg gcacaacaat atgccgataa
    19801 caaagagaaa gtggcgctgt taaaagcgct ttggcagtac gcggaagaga tcgtctaatt
    19861 tgaagcctcc gccgctggta cggcggaggt caatgttgag tcaggctttt tgaacggtga
    19921 tactccacgc tgcatcgcca atctgctgat aatcggtgat ggcatgccct tcttctgccg
    19981 cccactgtgg gattgcttca gttgcctggg tgcaatcaaa ctcaattacc aactcatcgc
    20041 cgctggccat ttctgccagt gctgcttttg cttctatgag tggaaacgga cagacctgcg
    20101 tcaccacatc cagcttttta atcgccatct ttacacctta cttaatttgc cgcagcagtt
    20161 gccagtcgcg ccttacgctg cggtcgaaca tacacaagcc aggacgcagt ccagactccg
    20221 agaatcataa atacaaggcc aatccagccc tgccaggtca tcattgcagt cataaccagt
    20281 ccattaccga taaagcaacc acctgcaata ctggcaccga agcccatcag tacaccacct
    20341 aacccgctac gtaatgttgt ttgtgcatca gctgcgcgaa cgcggaactc acggctcgct
    20401 ttggctgcaa taaatgaccc cacgaagatc cctaacacca ggaaaacgcc ccagttaatg
    20461 tatttcacgt cacccgcgac cagaaattgc agaatattgg ccgttgggga agtgattcct
    20521 aacccaaaca tgcgtccggt tgcttcactc aggggccagg ctaaaagcgc aatcaaaccg
    20581 atgagtacag cagtgacaaa gggatgccag cgtttttcaa acagaatatg agcgatccca
    20641 gttcggcgcg gcggtaaggt cgcgactttg agttttggct tcttcaactc tttcatcacc
    20701 acccagagcg ttatcaccag caaaacggca accaacggcc aaacagacaa attaaaagtc
    20761 tcagcaatag agttatgttc agtactgtag tgctgcaagg tttgatttaa accactggca
    20821 tgtggagaac gcatcaccgc actcatcacc atataagtga aaagcgcgat ccaactgccg
    20881 atcaatccct cacccgcgcg ataccaggtc ccggtggcac atccacccgc cagaacaatt
    20941 cccagcccga agatataccc acctataaca gtaccaagcc acgggaacgc ctcggcttcg
    21001 taagtcagta ggcccgcctg aattaacgca aaaaccccca cgctttgtac cgaaattgca
    21061 atcagcaagg cgtaaaacat gcgattattt ttcgcgatat acatatcgcg aaaaccacct
    21121 gtcaggcaaa aacgcccacg ctgcatgaca aatcccagca gagcaccaca aattagcccg
    21181 cttaatatca ttgaaaacat aaattaatta accagatgaa tgttaatgag gaaattattc
    21241 atgactggtg gaatgcagac caataaccaa attctctaaa ttagaacaaa tggttattaa
    21301 tgaggatttc gataaaaaaa aataaaaaaa ccggagccaa atattcattc gactccggtt
    21361 gattattaga aaagatgatt atgcaacgtc ttcgtactgc ggaactgggt tacggaagct
    21421 cttggtgacg caagccaggt aaatcagacc gatagcagcc caaatcagac ccagaaccat
    21481 tgagctttct tccaggttaa cccacagcgc accaacggtc agcgcaccac acatcggcag
    21541 gaacagatac tggaagtgat ctttcagcgt cttattacgc ttctcacgga tccagaactg
    21601 cgagatgacc gacaggttaa cgaaggtgaa cgccaccaac gcaccaaagt taatcagcgc
    21661 tgtagccatt accaggtcga agttgattgc cagcagggca atcgcgccaa ccaggatgat
    21721 gttcatcgcc ggagtacgcc atttcgggtg tacataaccg aagaagcttt tcgggaatac
    21781 gccgtcacga cccattacgt acatcagacg cgctacgcct gcatgcgccg ccataccgga
    21841 cgccagtacg gtaatggtgg agaagatcag cgcgccaacc tggaacgctt tacccgcaac
    21901 gtacagcatg atttcaggct gtgacgcatc cggatcttta aagcgagaga tatccgggaa
    21961 gtacagctgc aggaagtaag ttgcaaagat gaagatcatg ccgccaatca gcgcggtcag
    22021 gaaaatcgca cgcgggatca cgcgctctgc atctttggtt tcttccgaca ggttgctgat
    22081 gccgtcaaag ccggtaaagg aaaagcacag gattgtcgcc ccggtaatca tcggaataac
    22141 atgcgcatcg ccagaccaga acggacgagt gctcgccagc gtaccagcgc cttcaccttc
    22201 aaatacgcca taaacaacca tgcccagaat caccgcgatc agcactacct gcaacacgac
    22261 gattacagtg ttgaagttcg ctacggattt cagactacgc aggttaaagg cggtcataaa
    22321 ggccaccagc gccaccacaa acatccacga tgggatggaa ggcaccagag cttcaaaata
    22381 gattttcgcc agcagaatgt tgatcatcgg cgcgaacaga tagtcgagca gagaagacca
    22441 acccaccata aagccgacag tcgggctaat ggatttctgg gcgtaagtat atgcagagcc
    22501 agcagaagga tagcggcgaa ccagcttccc gtagctcaga gccgtaaaca ggatcgcaat
    22561 caatgcgaac gcataggctg tcggcacatg accatccgtg aggcctgaaa cgataccaaa
    22621 agtatcaaac agcgtcatcg gctgcatata ggcaagaccc atcattacaa ccggaactaa
    22681 cgtaagcgtt ttacgcaatt ccacgcgaga ggtgtttgga gtaacgttat gcgacatggt
    22741 cattctcctt tacggtgata accgtcgcgt aagcaaaaaa ttgccccatt tttttggatt
    22801 cctcagcgac aacaactgtc gatttttagt aaatatctat ccggtacgaa gcccggcctc
    22861 ttggtatgaa tgattggttt gaagcaaaaa ataaccgacg ctgatgaaac gtcggttttt
    22921 agtcattttt tgacagcggc gcattgtgcc taaatggggg ggaaatgaca agagaatgag
    22981 aagcttgtca taataatttt tctttaaatg gctgattttc cgtcatcaga ttttgcataa
    23041 acaccggcga tcgcactatt tgctaaaatt tcatcccgcc accagccgct ggcaagccct
    23101 gcggtctgtt cgttccagcc aatccagacg ggttcaaaga gagtttgggc gaccactttc
    23161 ttctcgatta acgcgccact atcgagaaaa cgttgcacca gataacgggg taaatagccg
    23221 cagccgaggc cgctaatttg cagctccagc ttggttttaa aatcgaaaac ggtaatggct
    23281 tcttgttcgt cgagaagttg tgaggccgta gaagcgcctg cctgcgcagt gtcccccacc
    23341 acaatcgccc gatatcgctt aatgatacga cggtttaacg gctcttcttc ctgggctaac
    23401 ggatgatgag gcgcgaccgc aaagacttgt tccagatcgc ccaaccgcga gaaaccaaac
    23461 tcactggaag atggtggctc atgcatcgcg ccaacaataa tatccgctcg cccctgagtc
    23521 agggcatccc aggaaccacc gagtacgcca ttgataaact tcaggcgcgt cacgctatga
    23581 tgttgataaa aagcttcaat cagtggcgca agcagagaaa aaggaaacgt gtcgtccacg
    23641 ccaatcacca gctcgttttc ccagccttca tgcagtttga tcgcctgctt ttccagttct
    23701 cgcacggtat gcagaacttc ccgccccttc tctaataaca tttttccggt acgggtgaat
    23761 ttagcgcggt gaccgctacg atcaagtaat tgaatattaa ggtcgctttc cagcttgtga
    23821 acggtgtaac tcagagcgga cggcgtcttg tagagtttag ccgacgccgc cgcaaaactg
    23881 ccttcttttt ctaaggcatc gaggatcatc aacacatcca gcagttgttt catacacgcc
    23941 cccttgcagt gcatgcggcg tcctgctggc gtgatcgttt actccagcgg cattaccagc
    24001 ggatcgggat actggtattc aaatcccagt tcattacaaa tccgactgcc atcaataatc
    24061 ttacctttgc cgctgtccag actatttctg aactgcggag gttccagccc cagtaaacgg
    24121 gccatctgcg gatagaaaac attacgcgca gggtgagctg gcgcacatat attatagatg
    24181 tgtccgcctt taggtgcctg taacaacaga gtgatagcgc caataacatc ttctaaatgg
    24241 actaaattaa caccatgttc accatcaggc gcggtttttc cggcaaagaa gcgtccggga
    24301 tgacgtcccg gtccgaccag ccccgcaaga cgcagaatat cgaccgaagt accgggtaaa
    24361 ttgtgcagcc agtcttcgag ttcttctaat actcgtccac tgttggtcac cggattacgc
    24421 ggggtggttt ctttcaccgt gccttgcgcg tcgccataga cagacgttga gctggtaaaa
    24481 ataatgcgag gaatacgatg ggccagcgcg ctatccacta actcttgtac cgcttgtaaa
    24541 tagaactcat cgccggggcc gctacgacgt gccggaagcg taatgaccag cgcatcggca
    24601 tccatcaggg catccagatc gtcagaatcg caaactaact caggctccat gcgaagcaga
    24661 tagctatcaa tgccactcat tcgggccgct tcgacaccat cttgtgtggt tttactcccg
    24721 gtgacttgcc agcctcgcgc tgaaagtgac atcgccagcg gcatgcctaa ccaccctaac
    24781 ccgacaattg cgaccttttt catccgttat ctcctgatac accttagatc tataaggcta
    24841 cgctagcgta tcaaaactga caattcattc tatgaatgaa tctgttcaat aatgataacg
    24901 acatgctgca atgagcagtg aatcgtcggt aaccgcgtat accagacggt gctcctctgt
    24961 aatgcgtcgg gaccagaaac ctgacaaatt atgtttcagg ggttctggct tccccttacc
    25021 ttcaaatggc gttctgcggg tatctttgat aagttcattg atctttttaa caattcgctt
    25081 atctgtttcc tgccagtaca gataatcatc ccatgattcc tcagaccaga ttagtttcac
    25141 tcaataatgt ccttttctgt tcctttgcct gatttcaggc tatcgattga gtccatcaat
    25201 ctccgggcgt tagcggggga gcgcagtaga taagccgtct cttccagcga gttgtattct
    25261 tcgagtgaca tcagaacaca agcctctcca ttctgacgag taataaggat cggggcatga
    25321 tcttcaacgg ctttcatcat tgttgccgac aaattctgac gcgcttcgct gtagctaatt
    25381 gtacgcatgt caatctcctc ttttgtacag tttattgtac aatgatgggc gttaattaac
    25441 tatttattaa ttagtttgta gatcaaggta ttgtcagtga gacgaaaatc caggcttcgc
    25501 tatttttggt gccgtcagct tagaggacag tcctcttagc cccctccttt ccccgctcat
    25561 tcattaaaca aatccattgc cataaaatat ataaaaaagc ccttgctttc taacgtgaaa
    25621 gtggtttagg ttaaaagaca tcagttgaat aaacattcac agagactttt atgacacgcg
    25681 ttcaatttaa acaccaccat catcaccatc atcctgacta gtctttcagg cgatgtgtgc
    25741 tggaagacat tcagatcttc cagtggtgca tgaacgcatg agaaagcccc cggaagatca
    25801 ccttccgggg gcttttttat tgcgcggttg ataacggttc agacaggttt aaagaggaat
    25861 aacaaaatga cagacaacac tcgtttacgc atagctatgc agaaatccgg ccgtttaagt
    25921 gatgactcac gcgaattact ggcgcgctgt ggcattaaaa ttaatcttca cacccagcgc
    25981 ctgatcgcga tggcagaaaa catgccgatt gatattctgc gcgtgcgtga cgacgacatt
    26041 cccggtctgg taatggatgg cgtggtagac cttgggatta tcggcgaaaa cgtgctggaa
    26101 gaagagctgc ttaaccgccg cgcccagggt gaagatccgc gctactttac cttgcgtcgt
    26161 ctggatttcg gcggctgccg cctttcgctg gcaacgccgg ttgatgaagc ctgggacggc
    26221 ccgctctcct taaacggtaa acgtatcgcc acctcttatc ctcacctgct caagcgttat
    26281 ctcgaccaga aaggcatctc ttttaaatcc tgcttactga acggttctgt tgaagtcgcc
    26341 ccgcgtgccg gactggcgga tgcgatttgc gatctggttt ccaccggtgc cacgctggaa
    26401 gcaaacggcc tgcgcgaagt cgaagttatc taccgctcga aagcctgcct tatccagcgc
    26461 gatggtgaaa tggaagaatc caaacagcag ctgatcgaca aactgctgac ccgtattcag
    26521 ggtgtgatcc aggcgcgcga atcaaaatac atcatgatgc acgcaccgac cgaacgtctg
    26581 gatgaagtca tcgccctgct gccaggtgcc gaacgcccaa ccattctgcc gctggcgggt
    26641 gaccaacaac gcgtagcgat gcacatggtc agtagcgaaa ccctgttctg ggaaacgatg
    26701 gaaaaactga aagcgctggg tgccagttca attctggtcc tgccgattga gaagatgatg
    26761 gagtgatcgc catgagcttt aacacaatca ttgactggaa tagctgtact gcggagcaac
    26821 aacgccagct gttaatgcgc ccggcgatct ccgcttctga aagcattacc cgcactgtta
    26881 acgatattct cgataacgtg aaagcacgcg gtgatgacgc cctgcgggaa tacagcgcga
    26941 agtttgataa aaccacggtt aacgcgctga aggtgtctgc tgaggagatt gccgccgcta
    27001 gcgaacgcct gagcgacgag ctaaaacagg cgatggcggt ggcagtaaaa aatattgaaa
    27061 ccttccacac tgcgcaaaaa ctgccgccgg tagatgtaga aacgcagcca ggcgtgcgtt
    27121 gccagcaggt cacgcgcccg gtcgcttcag ttgggttgta tattcctggc ggctccgccc
    27181 cgctcttctc aacggtatta atgctggcga ctccggcgcg tattgcgggc tgtaaaaaag
    27241 tggtgctgtg ctcaccgccg ccgattgccg atgagatcct ttatgcggcg cagctgtgcg
    27301 gtgtgaagga cgtgtttaac gtcggcggcg cacaggccat tgccgcgctg gcgtttggta
    27361 cggaatctgt gccgaaagtg gacaaaatct tcgggccggg taacgccttt gtcaccgaag
    27421 cgaaacgtca ggtgagccag cgtctggacg gtgcggctat cgatatgccc gcaggcccgt
    27481 cggaagtgtt ggtcattgcc gacagcggcg caacgccgga tttcgtggct tctgatttgc
    27541 tttctcaggc tgaacacggc ccggactcac aggtgatttt actgacgcct gacgctgata
    27601 tggcgcatca agttgccgaa gccgtcgaac gccagttagc agaactgccg cgtgccgaaa
    27661 ccgcccgcca ggcactgaac gccagccgcc tgatcgtgac taaagattta gcgcagtgcg
    27721 tagagatctc caaccagtac ggcccggagc acctgatcat ccagacccgc aacgcccgcg
    27781 atctggtcga tggcatcacc agcgccggtt cggtatttct tggtgactgg tcaccggaat
    27841 cggcaggtga ttacgcctcc ggcaccaacc acgttctgcc gacttacggt tacaccgcca
    27901 cctgttccag cctcgggctg gcagatttcc agaagcgtat gaccgtgcag gaactgtcga
    27961 aagaaggttt ctccgcgctg gcttcgacca ttgaaacatt ggccgccgcc gagcgcctga
    28021 ccgcccataa aaatgccgtt actttgcgtg ttaacgccct taaggagcaa gcatgagcac
    28081 cgtgactatt accgatttag cgcgtgaaaa cgtccgcaac ctgacgccgt atcagtcggc
    28141 gcgtcgtctg ggcggtaatg gcgacgtctg gctgaacgcc aacgaatacc ccactgccgt
    28201 ggagtttcag cttactcagc aaacgctcaa ccgctacccg gaatgtcagc cgaaagcggt
    28261 gattgaaaat tacgcgcaat atgcaggcgt aaaaccggaa caggtgctgg tcagtcgtgg
    28321 cgcggacgaa ggtattgaac tgctgattcg cgctttttgc gaaccgggta aagacgccat
    28381 cctctactgc ccgccaacgt acggcatgta cagcgtcagc gccgaaacga ttggcgtcga
    28441 gtgccgcaca gtgccgacgc aggacaactg gcaactggac ttacagggca tttccgacaa
    28501 gctggacggc gtaaaagtgg tctatgtttg cagccccaac aacccgacag ggcaactgat
    28561 caatccgcag gattttcgca ctctgctgga gctaacccgc ggaaaagcca ttgtggttgc
    28621 cgatgaagcc tatatcgagt tttgcccgca ggcatcgctg gctggctggc tgacggaata
    28681 tccgcacctg gctattttgc gcacactgtc gaaagctttt gctctggcgg gccttcgttg
    28741 cggatttacg ctggcaaacg aagaagtcat caacctgctg atgaaagtga tcgcccccta
    28801 cccgctctcg acgccggttg ccgacattgc ggcccaggcg ttaagcccgc agggaatcgt
    28861 cgctatgcgc gaacgagtga cgcaaattat tgcagaacgc gaatacctga ttgccgcact
    28921 gaaagagatc ccctgcgtag agcaggtttt cgactccgca accaactaca ttctggcgcg
    28981 ctttaaagcc tccagcgcag tgtttaaatc tttgtgggat cagggcatta tcttacgtga
    29041 tcagaataaa caaccctctt taagcggctg cctgcgaatt accgtcggaa cccgtgaaga
    29101 aagccagcgc gtcattgacg ccttacgtgc ggagcaagtt tgatgagtca gaagtatctt
    29161 tttatcgatc gcgatggaac cctgattagc gaaccgccga gtgattttca ggtggaccgt
    29221 tttgacaaac tcgcctttga accaggcgtg atcccggaac tgctgaagct gcaaaaagcg
    29281 ggctacaaac tggtaatgat caccaatcag gatggtctgg gaacacaaag tttcccgcag
    29341 gcggatttcg atggcccgca caacctgatg atgcagatct tcacctcgca aggcgtacag
    29401 tttgatgaag tgctgatttg tccgcacctg cccgccgatg agtgcgactg ccgtaagccg
    29461 aaagtaaaac tggtggaacg ttatctggct gagcaagcga tggatcgcgc taacagttat
    29521 gtgattggcg atcgcgcgac cgacattcaa ctggcggaaa acatgggcat taatggttta
    29581 cgctacgacc gcgaaatcct gaactggcca atgattggcg agcagctcac taaacgagac
    29641 cgttacgctc acgtagtgcg caacaccaaa gagacacaga ttgacgttca ggtgtggctg
    29701 gatcgcgaag gtggcagcaa gattaacacc ggtgttggct tctttgatca catgctggat
    29761 cagatcgcca cccacggtgg tttccgtatg gaaatcaacg tcaaaggcga cctctatatc
    29821 gacgatcacc acaccgtcga agataccggc ctggcgctgg gcgaagcgtt aaaaatggct
    29881 cttggcgaca aacgcggtat ttgccgcttt ggttttgtgc taccgatgga cgaatgcctt
    29941 gcccgctgcg cgctggatat ctctggtcgc ccgcacctgg aatacaaagc cgagtttacc
    30001 taccagcgcg tgggcgatct cagcaccgag atgatcgagc acttcttccg ttcgctctca
    30061 tacaccatgg gcgtgacgct acacctgaaa accaaaggta aaaacgatca ccaccgtgta
    30121 gagagcctgt tcaaagcctt tggtcgcacc ctgcgccaag ccatccgcgt ggaaggcgac
    30181 accctgccct cgtcgaaagg agtgctgtaa tgaacgtggt gatccttgat accggctgcg
    30241 ccaatctgaa ctcggtgaag tcagccattg cgcgccacgg ttatgaacca aaagtcagcc
    30301 gtgacccgga cgtggtgttg ctggccgata aactattttt acccggcgtt ggcactgcgc
    30361 aagcagcgat ggatcaggtg cgtgagcgcg agctgtttga tctcatcaaa gcctgtaccc
    30421 aaccggtact gggcatctgc ttagggatgc aactgctggg tcggcgcagc gaagagagca
    30481 acggcgtcga cttgctgggc atcattgacg aagacgtgcc gaaaatgacc gactttggtc
    30541 tgccgctgcc gcatatgggc tggaatcgcg tttacccgca ggcaggcaac cgcctgtttc
    30601 aggggattga agacggcgcg tacttttact ttgttcacag ttacgcaatg ccggtcaatc
    30661 cgtggaccat tgcccaatgt aattacggcg aaccgttcac cgcggcggta caaaaagata
    30721 acttctacgg cgtgcagttc cacccggagc gttctggtgc cgctggcgct aagttgctga
    30781 aaaacttcct ggagatgtga tgattattcc ggcattagat ttaatcgacg gcaccgtggt
    30841 gcgtctccat cagggcgatt acggcaaaca acgcgattac ggtaacgacc cgctgccgcg
    30901 cttacaggat tacgccgcgc agggtgccga agtgctgcat ctggtggatc tgaccggggc
    30961 aaaagatccg gcaaaacgtc aaatcccgct gattaaaacc ctggtcgcgg gcgttaacgt
    31021 tccggtgcag gttggcggcg gtgtgcgtag cgaagaagac gtggcggcgt tactggaagc
    31081 gggcgttgcg cgcgtggtgg ttggctccac cgcggtgaaa tcaccagaaa gggtgaaagg
    31141 ctggtttgaa cgtttcggtg ccgatgcctt agtgctggcg ctggatgtcc gtattgacga
    31201 gcaaggcaac aagcaggtgg cggtcagcgg ctggcaagag aactcgggcg tttcactgga
    31261 acaactggtg gaaacctatc tgcctgtcgg cctgaaacat gtgctgtgta ccgatatctc
    31321 gcgcgacggc acgctggcag gctctaacgt ctctttatat gaagaagtgt gcgccagata
    31381 cccgcaggtg gcatttcagt cctccggcgg tattggcgac attaacgatg ttgctgccct
    31441 gcgaggcact ggcgtgcgcg gcgtaatagt tggtcgtgca ttactggaag gtaaattcac
    31501 cgtgaaggag gccatcgcat gctggcaaaa cgcataatcc catgtctcga cgttcgtgat
    31561 ggtcaggtgg tgaaaggcgt acagtttcgc aaccatgaaa ttattggcga tattgtaccg
    31621 ctggcaaaac gctacgccga agaaggtgca gacgaactgg tgttctacga tatcaccgct
    31681 tccagcgatg gccgtgtggt agataaaagc tgggtatctc gcgtggcgga agtgatcgac
    31741 attccgttct gcgtggcggg tgggattaag tctctggaag atgccgcgaa aattctttcc
    31801 tttggcgcgg ataaaatttc catcaactcc cctgcgctgg cagacccaac cttaattact
    31861 cgcctggcgg atcgctttgg cgtgcaatgc attgtggtcg gcattgatac ctggtacgac
    31921 ggcgaaaccg gtaaatatca tgtgaatcaa tataccggcg atgaaagccg cacccgcgtc
    31981 actcagtggg aaacactcga ctgggtacag gaagtgcaaa aacgtggagc cggagaaatc
    32041 gtcctcaaca tgatgaatca ggacggcgtg cgtaacggtt acgatctcga acaactgaaa
    32101 aaagtgcgtg aagtttgcca cgtcccgcta attgcctccg gtggcgcggg caccatggaa
    32161 cacttcctcg aagccttccg cgatgccgac gttgacggcg cgctggctgc ttccgtattc
    32221 cacaaacaaa taatcaatat tggtgaatta aaagcgtacc tggcaacaca gggcgtggag
    32281 atcaggatat gttaacagaa caacaacgcc gcgaactgga ctgggaaaaa accgacggac
    32341 taatgccggt aattgtgcaa cacgcggtat ccggcgaagt gttaatgctg ggctatatga
    32401 acccggaagc cttagacaaa accatcgaaa gcggcaaagt caccttcttc tcgcgcacta
    32461 aacagcgact gtggaccaaa ggcgaaacgt cgggcaattt cctcaacgta gtaaatattg
    32521 cgccggactg cgacaacgac acgctactgg tactggcgaa tcctatcgga ccaacctgcc
    32581 acaaaggcac cagcagctgc ttcggcgaca ccgctcacca atggctgttc ctgtatcaac
    32641 tggaacaact gctcgccgaa cgcaaatctg ccgacccgga aacctcctat accgccaaac
    32701 tgtatgccag cggcaccaaa cgcattgcgc agaaagtggg cgaagaaggc gtagaaaccg
    32761 cactggcagc gacggtacat gaccgctttg agctgaccaa cgaggcatcg gacttgatgt
    32821 atcacctgct ggtgctgttg caggatcagg atctggattt aacgacggta attgagaacc
    32881 tgcgtaaacg gcatcagtga gttgcagggt aagcggatgc gatgttgttg tcgcatccgg
    32941 caaaaaaaaa gggcaaggtg tcaccaccct gcccgttttc tttaaagctg caaagattac
    33001 ttcgcgttat aattacgcag agcgttacgc cccagcacaa tccccgcgcc aaccatgccg
    33061 cccagcagca ccgccagaat caaggtaatt gcctttttcg ggctatcgcg acgaataggt
    33121 aacgtcggtt tcatcacata acggtaagca tgaatatcaa gatcatcaac ctttaagttt
    33181 tcaatatcca acaggttttg acgtgtctga tagtagtttg gtgagaacac caacggacgg
    33241 gtcgcctcat gcttaatcat cgactccagc gcttcgctcc ccaaaaggaa caacgtatct
    33301 tgtgtcacat cttcagtctg ctgaacctgt ggctttgcca cctgcgcctg attcgcatac
    33361 tgcaacgctt cctgaatctg acggatacgc agatctttct gctcctgcgc gactacttcc
    33421 tgcgttctta aagagtcctg caagtttttc cgtcccagag caatgttgtc tttgagatcc
    33481 ttttctagct cttgattcac tttatcatca acttgctgaa tgtattgggc caacttcatt
    33541 tgtgctccct ctgcagtttg cccaacataa gaaacagtca atggtaattg ctggttctta
    33601 acagaaggtt cgatggtaag tttttctggt tcttcctgat tatccagcgt ttctgctaat
    33661 gcagagaagg cagaactgaa gcgaccaatt aacgtctcct gtaaatccga tactttcggt
    33721 gcagcctgac catagataac attcatggca ttgttatagc cagcaatttg ccccacgtcg
    33781 ggctgagtga taattgctgt tgacgtccat ttctccttcg ctactgccaa atatccaata
    33841 gccagggcaa tagccacaat gacggaaatg atgattgtca tcttgccacg ccacaactgc
    33901 actagtaaat caatcaaatc aatctgttcc gggtcatggt tttgcccaga aacattatta
    33961 ttttctactc tcatcattac cctaactgac ggaaatttcc aagtccgcat agtttagcga
    34021 taatcctagt gaatgctata ggataagtga taaaaataat gaattattgg ggaggtaaca
    34081 aaagacaatc agggcgtaaa tagccctgat aacaagatgt tagtcgctgc caaagagatc
    34141 gcgggtgtat accttatctg ccacatcttt aagctcttct gccattcggt tagagataat
    34201 gacgtcggct tgttgtttga agatggcgag atcacgttcc agccgagagt tgaagaatga
    34261 gtcttctttc atcactggct cgtagatgat cacttcaacg cctttcgcct tgatacgctt
    34321 cataatcccc tgaatagaag acgcacggaa gttatctgaa ccgctcttca taatcagacg
    34381 ataaataccc accacttgcg gtttgcgtga caaaatggca tcggcaataa aatccttacg
    34441 cgtgcggttt gcatcgacaa ttgccgagat caggttattc ggcacagact gatagttcgc
    34501 cagtaactgc ttggtatctt tcggcaagca ataaccacca taaccaaacg acgggttgtt
    34561 gtaatggttg ccaatacgcg gatcgagaca aacgccttcg ataatctggc gagtattcag
    34621 acctaaactt tctgcgtagc tatccagttc gttaaagtat gccacgcgca tcgctaggta
    34681 ggtgtttgca aaaagtttaa tcgcttctgc ttcggtcgaa tcggtgaaca gggtcgggat
    34741 attttgctta atcgcgcctt cctgtaacaa cgcagcaaaa cgttctgcgc gttctgaacg
    34801 ctcaccgatg acaatacgtg aaggatggag attatcgtaa agggctttac cttcacggag
    34861 aaattccgga gagaaaataa tattttcagt acgatatttc ttatgcatcg cttcggtaaa
    34921 accaacggga accgttgatt tgatgaccat aaccgcataa ggatttatct caactacgtc
    34981 tttaattact gattctacac tggatgtatt gaaataatta gttttaggat catagtcggt
    35041 tggagtggcg atgatgacat aatcagcatc ccggtaggct tcatttttat ctaatgtggc
    35101 attaaagtgt attttatctg attgcaaaaa ctgctgaatt tccttatcaa caataggaga
    35161 tatccgatca ttcagcatag caacgcgtga cggtaaaata tctaatgcca caacctcatg
    35221 attttgtgcg attagaagcc cgtttgacaa gcctacatag ccagtaccgg aaatggtgat
    35281 tttcatttca tgctctcaga attaacttaa ctgtgaatca tgatgttttt agcatcctga
    35341 taagagctaa atgttttaac gctacgttat tagctggtca acgctacgaa aaacaattca
    35401 gattttcttt ataagaatag ctgatattta ttaagttaat attaagcagt gaaatttagt
    35461 ctataagata aatggcaaaa aaaaagcccg gtgcaatata cgccgggcct caattttact
    35521 gttggttaaa tcagattaat ccagccattc agtatggaac acaccttctt tatcaatgcg
    35581 cttataagta tgtgcaccga aatagtcacg ctgtgcctgg atcaggttcg caggcagaac
    35641 agcggcacgg tagctatcgt aataggcaac cgcagcggcg aaggtcggaa ccgggatacc
    35701 gttctgtact gcataagcaa cgacatcacg cagcgcctgc tggtagtcat cggcaatttg
    35761 cttgaagtac ggagccagca gcaggttagc gatctgcgga ttttcggcat atgcatcggt
    35821 gattttctgc aggaactgcg cacggatgat gcagccagca cggaaaatct tcgcgatttc
    35881 gccgtagttc agatcccagt tgtattcttc agacgcagca cgcagctgag agaagccctg
    35941 agcgtaagaa acgattttgc ccagatacag cgcacggcga actttttcga tgaactcagc
    36001 cttgtcgcct gctgactgag cttgcggacc agagagaact ttagatgcgg caacgcgctg
    36061 atctttcaga gaagagatat aacgtgcaaa cacagactcg gtaatcagcg acagcggttc
    36121 tccgagatcc agcgcgctct ggctggtcca tttaccggta cctttgttag ccgcttcatc
    36181 caggatcaca tcaaccaggt agttaccgtc ttcatctttt ttagtgaaga tatctttggt
    36241 gatgtcgatc aggtagctgc tcagttcacc gttattccac tcggtaaagg tctgtgccag
    36301 ttcttcgtta gagagattca ggccgccttt caggagagaa taggcttcag caatcagttg
    36361 catatcaccg tattcaatac cgttgtgaac catcttcaca taatggcctg cgccatcagc
    36421 accgatatag gtaacgcacg gttcgccatc ttcagcaacg gcggcgattt tggtcaggat
    36481 cggcgcaacc agttcatagg cttctttctg gccaccaggc atgatggacg gccctttcag
    36541 cgccccctct tctccgccgg aaacaccggt accgatgaag ttaaagcctt cggcagaaag
    36601 ctcacggtta cgacgaatgg tgtcctggaa gaaggtgtta ccaccatcga tgatgatgtc
    36661 gcctttatcg agatatggtt tgagcgaatc aatagcagca tccgtgcctg cacctgcttt
    36721 caccattaac aggatgcgac gaggcgtttc cagagattca acaaactctt tcaccgtata
    36781 gtaaggaacc agtttcttgc ctggattttc ggcaatcact tcttccgtct tttcacggga
    36841 acggttgaaa atagagacgg tataaccacg gctttcgatg ttgagcgcaa ggttgcgccc
    36901 catcactgcc ataccgacta cgccgatctg ttgctttgac attgtttact cctgtcaggg
    36961 ggtggtcacc gccgagtgtg cggcttaaat gtggtataga tgttaactca ctcatagaga
    37021 tggtgaatag cattaccttt gctataccac actgaatatc tcaccttttc cgtcttttaa
    37081 aacttattta tatgaaatta ctgaattact tgttcagtaa ctcaagaata agttttgtct
    37141 tttcttccat cagcggcaca tcaccgcgag attccacatt caaccgcacc accggttcgg
    37201 tgttggaggt gcgcaggtta aagcgccagt cggcaaaggt catgctgatg ccatcggtgc
    37261 gatccaccgc cagcgcctcg cggctaaaat gctgttccac gcggttaatc gcctcaacgg
    37321 gttgcgccag tttgctgttg atctcaccgc ttgccggaaa cgctgccatc cggtcgcgca
    37381 ccagttcgcc cagcgttttt cctttcaggc acaccagttc ggcgaccagc agccacggga
    37441 tcatgccgct gtcgcagtaa gcgaaatcac ggaagtaatg gtgggcgctc atttcaccac
    37501 cgtagatggc gtcttccttg cgcatccgtt ctttaataaa ggcgtgtccg gttttcgaca
    37561 tcaccggcgt gccgcctgcg gcagtcacca catcaacggt gttccaggag agacgcggat
    37621 cgtggatgat cttcgcgccg ggattttttt cgaggaatgc ttctgccagc aggccgacaa
    37681 tgtagtagcc ctcaataaac tgcccttttt cgtcaaacag gaagcagcgg tcaaaatcgc
    37741 catcaaaagc aatgcccata tccgcaccgt gtttgatgac cgcattgcgg gtgtcgtcgc
    37801 ggcattccgg cagtagcggg ttaggaatac cgttggggaa attgccgtcc ggcgtgttgt
    37861 gcactttgat taattccacg ggtgcgccaa gggctttaaa gcgggcttcg atggcgtcca
    37921 ccaccggacc cgctgcgcca ttcccggagt taatcaccag cttgagcggc gtgaggtttt
    37981 tgacattgat ataaccgaac aggtgatcaa cgtaagcgtc acgcagattg atttgctgat
    38041 agcgaccgcg tttggtttca tcaacgggag gaaagtcgtt ggcttctgcc agacgctgga
    38101 catcgcgcag tccggtatca ccgctgatcg ggcgagcccc ttcgcgcacc agtttcatgc
    38161 cgttgtaatc catcgggtta tggctggcgg taacttcaat gccgccatcc acgccgagat
    38221 ggaacgtggc gaaatagatc tcttcggtgc cggacatgcc aatatccagc acatcgacgc
    38281 ccgcatcctg taaacctctc gccagcgcca gttttaaggt ttcgctggtg aggcggacat
    38341 caccgcctaa cacaatggtt ttcggtttga gaaattcgcc ataggcgcga ccaatgcgcc
    38401 aggcgatatc ttcattcagt tcttcgccta atttcccgcg aatatcatag gctttaaagc
    38461 aggttaattt tttcatgtcg ttaccctttt tcaggcaata gttggccctg accgaaacgg
    38521 ccaattttgt ttttgtcgtt atttacatct cggtttcgca ttcattgcct gatgcgacgt
    38581 ttacacccgt ccgtagcgat ccgcgaaacg caccacatca tcctcttcga gataagagcc
    38641 ggagcgcact tcaattaaat cgagcggaat tttccccggg ttttccaggc aatgcatcgc
    38701 ccccagcgga atataaatgg actcgttttc accaagcagt ttgatatcac catcaatggt
    38761 gacttttgcc gttcccgcga caaccaccca gtgttccgcg cggtgatggt gcatctgtac
    38821 cgacaagccc tcgcccggtt tcacggtgat gcgtttcacc tggtagcggt cgcccgcgtc
    38881 gatagagtca tatttgcccc acgggcgata gacttcgcga tgcacccgat gctcatggcg
    38941 accatcggct ttgatctgct cgaccacttt tttcacatcc tgcaccgcgt tacggtcggc
    39001 aatcagcacc gcatctttgg tctgcaccac taccagatct ttcaccccga cggtggttac
    39061 gaggccagat tcggcgtaca catagctgtt ttcagttttg tgattaatca catcgccgtg
    39121 gcaaacgttg ccctcggcgg tgtgggcgct aatttcccat aatgaggacc aggagccgac
    39181 atcgctccag cccgcatcca tcggcaccac aacggcatct gccgtacgtt ccatgaccgc
    39241 gtaatccacc gactcttccg ggcaggcgag aaacgcttct tcatccacac gaataaaatc
    39301 gagatccgga tcgacggcgc tcatcgcttt ttcacaggca tcgagaatat ccggacgata
    39361 ttttttcagt tcttcgagat agcgtccggc gcggaacagg aacatgccgc tgttccagta
    39421 atattcgccg cttgccacat aggcctgggc ggtttccaga ttcggttttt cgacaaactg
    39481 cgccacttca aaggccaccg tatcctgctc acccgcagac acttcaccgc gacgaatata
    39541 gccataaccg gtttctggta gatccggcac aatgccgaag gtcaccagct tgcccgcttc
    39601 ggcatatggc atggcattac gcacggcggc acggaacgcg tcttcatcgg caatcacatg
    39661 atccgccgcc aataccagca ttaacgggtc gctctccggg ctatgacgtt ttgccgccag
    39721 cgccgccagc gcaatggcag gtgccgtgtt tcgccctgcc ggttcgagaa taatgttctc
    39781 ggtaagtttg ttcagttgac gcagctgttc cgcgacaata aagcggtgct gctcattgca
    39841 aatcaccacc gggctttcgc actccacgcc gttcaggcgg cagatggtgg tttgcagcat
    39901 ggtgagatcg cctttcaggc ataaaaactg cttggggtaa agtacgcggg aaagcggcca
    39961 taagcggcta ccggagccac ctgccatcac aactggatag agtttcgact gcgccataat
    40021 tatccccgaa tatcatttat aaattgacgt aacacgttct ctttatcgag tgtgcgttcg
    40081 gcatattcac gtgccaccgt gttgtgtttg ggcagcagga gcgcctgacg gatccccgcc
    40141 accagcgcct cgaccgattc cggttctacg caaaccgcaa tgcccggaaa ggtttcgcaa
    40201 agctgcccca gttctgtgtg ggcctctgcg gtaatcaccg cgttaccgcc taccgccaga
    40261 atattggtca gtttcgacgg caatacggca tctgccgcgc cgcgtttttg caccaccaga
    40321 tggcaatcgc ccatcttcag cagtgcgggt aaagcgtcat acgattgcag cgggaaaaat
    40381 tgcatgttgc gcagtccacg ctgctgcgcc attttttcca gccgcgcttt gccgccgccc
    40441 tgcccgacaa tggcaaaaat cagcggttca tcgcgcagac gatcggcagc ttcaataacg
    40501 ttttccagcc cctgcttttc accaatattg ccggagtaaa gaatgatttt tttgttatcc
    40561 ggcaggtcaa gctggttacg aagggcgtca acatcggcat ctgcaacatg ctgaaaacgg
    40621 gcaatttccg accagttggg gaagaagatg acgttttccg ccgccacgcc tttttcgatg
    40681 gctttattca tcatcgaacg cgaaatcgtg gagacgttat cgacgttatg cagtccgcta
    40741 cgttcgaacg ccgttgccag ctgtgccact ttgctgcctt tgccttttcc ggcaaggccc
    40801 agccccagca tggcgtccac ttcgtaatcc tgaatatgca gcacggtacg cgcaccagaa
    40861 agtttcgcca gcaggcgcat tcccggcgtg caaaacagcg ttggcaccac gccgataatg
    40921 cgatccggct tccagcgacg ttgcgccatc agaggaaaga aactgctgac ggcaaaactg
    40981 ccgagatgta acaggcgttt cagggtgctc ggctgttttg gcacgtacag cgggcagcgc
    41041 cacaccgtgg cggccccctc ttctcgtttg taccgccagg cggaatagtt ctcgcccacc
    41101 tgccactgcg ggtagtaagg cggtgcggta ataacccgca cctcatgacc ttgtgccgcc
    41161 agccattcca ccatctcgcc ggtgtatttg ccgataccgg ttaactccgg cgagtagtta
    41221 atgccgtaga ccagtatttt cataatccgg gtactccggc acgcttctca gcgagaaaat
    41281 aggcgcggct gttagcgtga acatcgttac tggcgagcag cgcgtccggc gtcagccagc
    41341 ggtaatcgtc atgctgctca tccggcagta tcagctcttc ttcagcgact ctgaagcgaa
    41401 aaccgagcac cacatagtga gtggtgaaat ccgtgccgga gaagttatcg tcataaaagt
    41461 gctgccagac accgtaaaac tggcctgctg ttatcggcag acgcagcccc agttccgcca
    41521 tcgtcagccg ctcgaatgcg gcttccagcg tttcgtcttt ctgcacgcgc cctcccggca
    41581 caaaccagta accctgcgcc gggcggttgg ttcttttgcc aagcagaaac tcaccgcgac
    41641 tgttctcgac aataaagtcg agagagacaa gcggggtgga gcgcactacc gtggcaaagt
    41701 cttcctgacg taaaaacatc attacccccg aaagcggtct tgattctcaa ggaaccactg
    41761 gtaagtgctg gcaagccccg cttccagtga gatttcgtga taccagccaa gctgatgcag
    41821 gcgcgtcaca tccagcagtt tgcgcggcgt accatccggt ttgctggcat caaaaaccac
    41881 ccgacctttg taacccacca ctttggcgat ggtttgcgcc agctcgcgga tagtgcagtc
    41941 aacgcccgtg ccgacgttaa tgtgcgacag catcggctgg gtgttctcca gccagacttc
    42001 gtgcgccagc tccatgacat gaatgctcgc cgccgccata tcatcgacgt gcaggaattc
    42061 acgcatcggt gtaccgctgc cccataccac cacgtccggt gcattctgtg ccgtcgcctc
    42121 gtggaagcga cgcagcaatg cagggatcac atgcgaatta ctcgggtgga agttgtcatg
    42181 cgggccatac aggttggtcg gcatgactga gcggtaatcg cgtccgtact ggcggttgta
    42241 tgattcgcac agtttgatcc cggcgatttt ggcaatggcg taaggctcgt tggtcggctc
    42301 cagcgtgccc tgcaataact cgctttctgc catcggctgt tttgccagtt tcgggtagat
    42361 acaggacgac ccaagaaaca gcagtttgtt cacgtcgttc tgatgcgcgg cgtgaatgat
    42421 gttgctctca atcatcatgt tctggtagat gaaatccgcc ggataggtgt tgttggcaac
    42481 aatgccgccc actttcgccg ccgccagata gacctggtca atgcgttcgc tggcaaagaa
    42541 atcatgcacc gcgcggctgt ccagcaggtt cagctcgtcg cgggtgcgta ataccagttc
    42601 cacatcaccg cgctgttcga gctgccgcct gatggcagaa ccgaccatcc cacgatggcc
    42661 agcgataaaa atgcgttgtt tactcatgct tatgactcca gcgcgatcgc cacgtcgtag
    42721 ccgtgagatt tcagcagaga gtgttttttc gccgcttcga ggtcattagc caccatttca
    42781 gacaccatct ctctgagggt gatttccggt ttccagccca gtttttcgtg cgctttggtc
    42841 gggtcgccga gcagcgtttc aacttcagcc ggacggaagt aacgcggatc aacggcgata
    42901 atcacatcac ccggtttaac gcccggcgcg tcatgcccgg tgacggaaac cacaatgccc
    42961 ttctcttcaa cacccgtgcc ttcaaagcgc agtttgatgc ccagctgtgc cgctgccatt
    43021 tccacgaact gacgcacgga gtactgaacg ccggtcgcga taacgaaatc ttccggctgt
    43081 tcctgttgca gcatcatcca ctgcattttt acgtagtctt tggcgtggcc ccagtcacgc
    43141 agggaatcca tattgccgag gtacaggcac gactccagcc cctgggcgat gttggcgatt
    43201 gcgcgggtga ttttgcgggt aacgaaggtt tcgccgcggc gcggggattc atggttgaag
    43261 agaattccgt tacaagcgta catgccgtag gattcgcggt agttaacggt gatccagtag
    43321 gcgtacagtt tggcgaccgc atacggagat cgcgggtaga acggcgtggt ttctttctgc
    43381 ggaatttcct gcaccagacc atacagttca gaggtggaag cctgatagaa acgagttttc
    43441 ttttccagac cgaggaagcg gatcgcctcc agcaggcgca gcgtacccat cgcgtcgacg
    43501 tcagcggtat attctggtga ctcaaaagag accgcaacgt ggctcattgc gcccaggttg
    43561 tacacttcat ccggctgtac ttcacgcaaa atgcgcgtca ggttagaggt atcactcagg
    43621 tcgccataat gcagatggaa tttcgggttg caggtgtgcg gatcctgata aatgtgatcc
    43681 acgcgctcgg tgttgaatga cgatgcgcga cgcttaatac catgcacctc gtaacctttt
    43741 tccagcagaa actctgccag gtaagaaccg tcttgtccgg ttacaccggt gatgagagcg
    43801 acttttgaca tgtcttattc ctctgtattt ttgaatttat tcagtttcaa cgcgttcgcg
    43861 tatcaccact gcgggattcc cccggcaaac cacatttgcc ggaagcgatt taaaaacact
    43921 gcttcgtgca cccacgacgg tgccgtcgcc gattgtgacg cctggggcaa caaagacatc
    43981 ggttgccagc cagcatttct cgccaatcac aataggcgtg gcgttaatgg tgaaatgttg
    44041 acttgcatgg tcgtggctac cggtgcataa ataacttttt tgcgatatca ccgaatgtgc
    44101 gccaatggtt atttcaccga gggtatataa attgacgtca tcgccgaccc acgcgtaatc
    44161 acctaaggtt aatttccacg gataggtaat ttttactgac ggacgaataa ctacgttttt
    44221 tcctattttt gctccgaata aacgtaataa aaaagcccgc cagcgataca atacttgtgg
    44281 cgaccaggca aatattgttg cctgtactgc ccaccataat tgcactttaa tagcgttgcc
    44341 gccccggaac cctttcggca ccgagaaacc gcttaaatct tgcattggtt ttccttatat
    44401 tcagactttg ttatataagg ctttcgtctt tgaggtagta cgttggcgta aatgccagga
    44461 taattcagcc caaaagccag gcacatgtaa tatttgtcgt tggacttttt tcgcgtcagc
    44521 acacaattcc atattattgg tggtagatac cccacccatg gaaaattcag acaccaggcc
    44581 attgagtttt ttaaatgcat aaccggcttt atacattttg gctgccagcg cgtagtcgga
    44641 agaaacttta tattccaggt cataacgcca ttttttcaag ccggatacag ggaaaaatat
    44701 cgcctgatga ctggcgggca ggctgtgata aatataccag cccggtttgg cgctacgttt
    44761 aattttatgc ccgtcgccaa aatccagcag cgcatcgccg gtgatcatca tgttatcttt
    44821 ttgcattttt aacttacgga caaaatttgc ggcatcctga tgaaaaatat cgcccgaatt
    44881 gagaaacaac gcgaacttgc cttgtgccat cgtaatgcct ttgttcatgg cgtcgtagat
    44941 accgttatct ggctcgctga caaagcgtag gttatagata ccattgagat tttccagata
    45001 ctcacgggtg ccgtcattgg aaccgccatc gacaacaatc cattcgaagc tgatatcttc
    45061 cgcctgcgcc agatgcgcca gcgaggcatg tgttttgact atcccttcga ggttacgaaa
    45121 cgcgacagtg attatgctaa gcaacatgtt cttattctta cctcgtaata tttaacgctt
    45181 ttcgcaaaat aaatggacag acgattaaaa atgcatattc cgggctaaat atcgaaccgg
    45241 taaaaaacag cgataccgga gtaaaaagat ataattgcac gcgaaaattg cggttatcac
    45301 caaaagcgtt gatcatcatt tttatcactt tccccatgta ccacagcgat aaaaacactg
    45361 cgaaccagga aaaataaata atcagcagat acaaaccatt gtctatggtt tttccgacat
    45421 ccgcaccgtt aaatattccg aatgatgcga cgtattcata aagtgagcca aatctgacta
    45481 caccgtcaat atgggtcaaa gaataaccga ccatcaccag cggaccgaca atacgataat
    45541 acgatgacga tccttccgta cctaaatcgc ccagacgggt ggaaatataa ggaaacgcga
    45601 ttaccacacc aaccaggaat acagccagag aaatcaatgc caacggtaac tttttcttaa
    45661 tcgcctcttt attcagatat tgaaatgccc actccagcaa ataaaacagg ataaaagtca
    45721 taacccctga aaacgatccg gataatatta tccctgcgag aatcatagca tcggttttag
    45781 gcgttttgat accaaactgt ttgatgctga gccaaattga gattaatgcc agagcgaaaa
    45841 atgccggttc gaaataaagt gctgtcgtgc gcttgccgcc gaatttaatg aaattcagta
    45901 catagctgtt gctgtaaatc agatatttcg aaatcgactc cataatactg ctgccaccgg
    45961 tgaggataat ttgcgccatc tccaccgccg ccagcgccac caccagcccg atcaccagat
    46021 aaaagaaacg taatatcttg cgatggttgt gtggcgaaat cgttttaaag cgaatactcc
    46081 acaccatgcc aataatgatc acaatataga caaacagcat ggttgaagtg acgtatttac
    46141 tggcatccag cgactgacca aacagatagt taaacgccgt gagtcccgca ccaatcccta
    46201 aggcaatcat caatttttta acgctgatgc gttctaaaaa cagcagtaac aagacaggta
    46261 aaaaagtgac gatggtgatg gggaaacttt cgccaagctg ggcgattttg acgttaacca
    46321 gcaggtagat cagcggcagc agcaggtagc tacagattct gatagaagtt gacatactcc
    46381 tccagcatct gttgtccact gtaggtggcg cggctgcgtt ggctgaactc agccagcgtg
    46441 gtaccaaata tcgcctgcgc gatttccggt ttgcttaact gcaccagttg cagcacgtct
    46501 tcttcgtcga cggttttacc gccggatttt tgcaacactt cccgtgccgc gtcgctatgg
    46561 gtggcaatca ccggcacgcc aattgataac gcctcacaca aaatcagcgg gtagttatcg
    46621 acgcgagaac tgaataccag cgcatccatc tgattgagcg cgctcatcaa cttgcgcttg
    46681 tcagtttcaa agccgtgatt aaccacgttg ccagcggtga acggcgagaa cttaccaaag
    46741 gtatgcagtt cgattttgtc gcccagcgcc atcatctcac gcaccagttg ctggttagtc
    46801 ttgccgtcgt aacgcaagtc atgcgccacc accgcgattt tcggcttgcc ctgggtttcg
    46861 cgcaccggag gcaagtccgc cagaatcgct tcggttgcca tatcaatacc attattgata
    46921 atccggcaac gccctggacc gtacaggcta ttgaaagcgt cagccacatg ctggctgggg
    46981 gaaataaact gacagcccag cgccagcatc tcacggaata actggcgttt gcccgccacc
    47041 agctggtgtg cgcgatcaat cttcaccggc ggatagttat ttaaggtcgg gcatttctgg
    47101 cagcccgttt tccagccttc gcaaccgtcg gtaaaggcgc agcgtccggt aacactccag
    47161 tggtcgtgca gcgtccagac cagagtgacg tccggtttat ggtttttcac tttttcgcaa
    47221 aagcgcacca cgctcttaag atttagccag tagctgtgca gcacatgaaa atgcaggacc
    47281 accggacccg gtgtgcgagt aatggtgcga tataactcat tgaaattgcc aaacagatcg
    47341 cgattaaaca gacgaaacag ggcaatattc gccatcgcgg tcatccgcgg cgtatgtttg
    47401 atgacctgcg gatagttctg atggctgacg ctctctttgc cgcctttgcc gtaaccgtag
    47461 acaaaatgtg acgccagccc ctgttgcagc gcacgctggt ggagatctaa cgccacacct
    47521 gctgccccgc cttccgccag tcgcacatta aattgcagaa tattcattta attaccttca
    47581 ctcgcgcttt ttctcccacc accagcgcgt tgtccgggac agagtcgagc accacgctgc
    47641 ccgcacccac ggtgacgttg ttaccaagcg tgatatcacc aagaataatg acgttggcac
    47701 cgagttcgac gccgttgcca atgtgtggac atgccatgtt atcggcacca cgattgccga
    47761 tagtgacgcc gtggcgaatg gtgaaatcat cccccgctac cacgttttta ttgatcacta
    47821 cggcgtaacc gtgatggata gtaaagcggc ggccaatggt cgcggcggcc tggatttcat
    47881 aaccgaaaaa gcattcggtg ataatgcgat acagcaccag cagcggggcc gcccacagat
    47941 tgttgaggac gttctttttg cgccacaccg aacaaaaatg agcaacacga taggcaagaa
    48001 ccatgcagca cgggcgtaaa ctccagctgt tggcacgcag atcttccagc atctttagcg
    48061 cccccgaata ccatcagcca gacgtttgcc gttacgcacc gacagcagcg tcagcaaagt
    48121 gcgccaggtc atacgtttat tgcgaatctg gtagagggta aacagctgat attttttgct
    48181 tgcacggtca aatttatctt tgtgctttcg gtaaaagtgg aagtacccgg agaatttttt
    48241 cggtgacgag gtgatctgca tttcaccgtg attgatatgc aggatctgcg tcgcttcttc
    48301 cactttccac ggttcgccgt actccaccac catccgcagg aagatgtcgt aatcctgtgc
    48361 tgctttcagt tcggtatcga acaaacactc tttgaaacgc catgcccaag taaagacctg
    48421 attgccaatg atattgcgtt tatagaacag gcggcgtgag tacggcgatt tgggatacag
    48481 tggtaagctt gccggttggg aataaacttc tccctggcat acgtagtcgt tagcgtacaa
    48541 aaaggcgtga gtgaccagtt gctgtttatg ggcgaggaag acgctcagac ggttgggtgt
    48601 ccattcgtca tcgtcatcga tcccggtaat gtattcccct tgcgccagca taatcgcctg
    48661 gttacgtacc gcgcacgccc cgctgttaat gtcgttgtga atgtaggtga tacgcggatc
    48721 gttgagggca gtgacgtact gttgcagttg ttcccaggag gtggagcaat catccacgat
    48781 gatcatctcc cagttgctgt agtcctggcg caggaccgat tttatcgccc gaatcgccag
    48841 ttgttggcgg ttccaggtcg gcatatagat tgaaatcagc ggattctctt tcataggttg
    48901 ttctcccggc ttatgagctg gtttgcttgc cggatgcggc gtgagcgcct tatccggcct
    48961 acggggcggt gcgaatgcag gcgtcgttat ttcgcatccg acttatattc gtattcgtaa
    49021 tagccataat cctgatacgc gctggcgcgg cggaagatgg agttcagaat cacccctttc
    49081 accggaatac cgttttgctc aaagcggctc agactggttt ccacttcttt caatgtgttg
    49141 accgcatagc gcgccaccat taacgtggtt ccagcgtgac gcccgacaat tgctgcatcg
    49201 gtcactgcca gaatcggcgg cgtatcaatc aacaccaggt cgtagttttt actcgcccag
    49261 ctcaccaatt cggcaaagcg ttcgctcatc aacagttcag aaggatttgg cggcacctga
    49321 ccgcgcggga tcaggtcaaa tttggcaata gaggtcggtt tagctgcggt agtaatatcg
    49381 ccctggccaa tcagaatttc cgacaggcca ttgacgttat tggtacccaa cagctcgtgg
    49441 gtgtagcctt tgcgcatatc gcagtcgatc aacaacaccc gtttattggt ctgactgatc
    49501 accgccgcca ggttggcgca gacaaaggtt ttaccgattg acgggctcac ccctgtcatc
    49561 atcaacacat tgttctgcgc ctgcatcatc gcgaagtgca gactggtacg cagactgcgg
    49621 atggcttcaa tcgccagatc ggttggattc cccaccgcca gtagctggct ctgtttatag
    49681 cgtttaatcc ctttgatggt tttgacgcta tcgcgcgctt tctgccattc cgacagcggg
    49741 atgctggcat agacgctgat accatgttct tccagcacct gcgggctttc gatgccgcga
    49801 ttaaacaacg agcgcagcag cacacccacg atagagagca tcaggccgag gataattgcg
    49861 ccgaggataa tcagtccttt cttcggtttc agcacgccag gctgggtgat tgccgggtca
    49921 acaatgcgca catcgccgac ggtgctggct tcggtgattt tcagctcctg ctctttattc
    49981 agcagttgca tatagacctg ctgaccagac tcgacatcgc gggtcaggcg cacaatctcc
    50041 tgctgggttt tcggcatcgc cgttacgcga ccgttaagtt tggctttctc ttcttccagc
    50101 gcctgacgtt tctccagcag cgtgcggtac gccgggtgaa ctttggtgta cagcttggag
    50161 atttccgcct ctttaaaggt cagttcgttc aactgcgcgt cgatgttcac catcgaatcg
    50221 agcaccgctt tcgcttccag cggcagatca acagaatctt tatcctgacg gaaggcattc
    50281 agtttgtttt cggcaacatc gaggcggcta cgtacttccg gtaactgttg cgcgaggaaa
    50341 gcgaggcttt tcgacgcttc cgccgatttg cgctcaatat tttgttcctg atagttacgg
    50401 gcgatgctgt taagaatgtc gcggatctgt tcgcgatctt caccggtata agtcaggctc
    50461 agtacgcctg cgtctttgcc gttctccgtt accgtcaggc tgttttgcag ttggttgatc
    50521 atccccaacg tggagtattt ggtgacggta aactcactgc ccgggcgggc gtgaatggct
    50581 tcaaccatca gcgtgacgcc ttcttttttc agcatctggc ccgcttgccc acgggcgcta
    50641 aagccgccat cactgctcag ggtgtagttt ttattgtcca gcacattaag cgtaaacacc
    50701 tggcccgcca tctctttcgg gcggttaaag gtagtcactt tcaccgtctc gttctggcgt
    50761 cccatcagtc gctcccagcc cgccccgaaa atcgggaacg tgtttttgct cactgcaata
    50821 tcgaggtcga gatcgtccac cgttttacca agcaccagac gcgaacgaat caactggatc
    50881 tcggcgtccg atgcaggcgg tttgttggct aatgccgaac caatgtcctg cactaacgaa
    50941 ttgccgctgt tttgctcgat ttgtaccagt gcgtcggcac tataaatcgg cgttgcgaag
    51001 aaggtgtaaa ccacggcaca gagggcaaat acagcggtga tgccaatcac ccaccagcgc
    51061 gcttcaatga cggtgccgac caggcgacca atatcgattt catcactgcc cgttaccgga
    51121 gcggcatgtt gttttacttt ttctgtcatt cttatacctg ctctgcgttc aatgcctgcg
    51181 cccactggcg ggcagaccgt tcaagtaatg tgtacaccgc tgcaaacgtt tcccggcttt
    51241 tgcgatacgg atcggggatt tcacattcgt tatcccagtg accaaacagc atcactttgc
    51301 cgcgcatctc aggtgccatc tcgcataagc gttcgatatg gcgcttttcc atggtcaaaa
    51361 tcaggtcata gttgcgacac aggcggcggc tgatttgtcg tgcacagtgg ccttccagag
    51421 acagttgatg ttctgcggcg acgctgatag cggtaggatc agcgccctta ccgaccagcg
    51481 cgccgagtcc agcggactcc actttcagtt ccgggtgata acgttgcagt aagcgttccg
    51541 ccgtcgggga acggcaaata ttgccgacac agacaactaa tatgttgtta aacatgacga
    51601 ttaccagtta tgaatgtcgc tggctgtatc cgtcatgtaa cggacaccgc taatagttgg
    51661 caacaactga ttgatcagac ggttccagcg ggaaaccggg gcggtggtga catacaccac
    51721 gtcataaggt tgcaggcgga attctgtcgc catcaccagc gacgtggcat cggacatatc
    51781 cagctggtag atattggcaa tcttgccatt acgcccgccc tcgcctttca gcggacgaat
    51841 gacaaagatg ccgctggcgt tggaggtggt catgtcgata ccttcagcat tacccagggc
    51901 ttcagtcagg gtcatgccgc taaagtccat tttcagggtg ctctgtttct tcacttcacc
    51961 catcacaaat actttcagat catcattacg cggcacgtag agaatatcgc cgggataaag
    52021 cagtcggttc tgattgaggt cgccgttttg catcagcgcc tgcaaagaaa tgcgttcttc
    52081 acgaccattg tgtgttagca ctacgttgcg ccagtcagcg gtgtcggtca ggccacctgc
    52141 ggcgttgatg gcgtcgagaa tagtcagtgg cacgttggtg atcgcctgtt gaccggattt
    52201 attcacctga ccggagatat aggccttttg tgagcggaag gcggcgatat taacgtccac
    52261 ctgcgggtca gcgatgtacg tcgctaagcg cccggtaata tcactgcgga tttcagcgag
    52321 cgttttcccg actacgtgaa ccttgccgat atacgggtaa aacatagtgc cgtcaggctg
    52381 tacccagttg ccggtgtcgc tggagctgcg gtactgaccg gctggcgtgg tgagttccgg
    52441 gtgatcccag acggtgacat tcagaacgtc gcccggcccg acgcgatact ggtaattcgc
    52501 gatctcgctt tccagcgtca tattggggcg cgctacattc gggcgtgggc gtaattggtc
    52561 aatcaggcgc ggggtcagcg gataaacatt caccattttg tcgagatcga aatcagcgtc
    52621 ctgctgtttg atgacgtctt tgcccatcgt cgacatattg ctgcccggaa gtactgtgca
    52681 accgcttatc aaggttactg acaccaataa tggcatcaat ttcattttgg atttcatcat
    52741 tgtttattta tcactttggc agagtaatta tcctgtgcac tattaatagc aatatcgcca
    52801 tgcacattta ccttgcagtt aattgaataa aaatttaact ggcatcagtc ctaaaaaaat
    52861 tgatttcatc cgcaggctat tgacagaata attcagactg gtctttcagg catccagaca
    52921 cgctaccgcc cctggctttt tagccaccaa tacactgatt tagcttaatt tttcaaaccc
    52981 tctctgcata caatcgttga tgagaaaggg ttattgcgga aattaacttc cgaatataag
    53041 gtgacattat ggtaattgaa tattggctct ccaataatgc aggaggaagt gttacagcta
    53101 acggaatagc aggcaaggta acaattcggc aattggctat ttttaagaat tatattaagt
    53161 gtcattcaat atggttttta ggagtttctt taggttgaca atatttaata ttgcgcctcc
    53221 acatgcgata tttcttaaat aatgttttat tattaccact tttaattcag ggataatgtg
    53281 aggttattac cctcaaataa tatgagataa atagtgctgc aacattgcat ttttgccccg
    53341 atttatccat gatcgaattg tgacatttgt catacaacga ataggttttg tacttactat
    53401 ggaatggatt gccgatccgt ctatctgggc cgggttaatc acgctgattg tgatcgaact
    53461 ggtcctcggc attgataacc tggtctttat tgccatcctc gccgaaaaac taccgccgag
    53521 gcagcgcgac cgcgcacggg ttaccgggct gctgctggcg atgttaatgc gcctgttact
    53581 gctggcgtca atctcctggc tggtcaccct gactcaaccg ctgttcagct tccgctcgtt
    53641 tacctttagc gcccgcgact taatcatgct gtttggtggt ttcttcctgt tgttcaaagc
    53701 cacgatggag ctgaacgaac ggctggaagg gaaagacagc aataatccca cacaacgcaa
    53761 aggtgcgaag ttctggggcg tggtgacgca aattgtggta ctggacgcca ttttctcact
    53821 cgactcagtg attaccgccg tcgggatggt cgaccattta ctggtcatga tggccgccgt
    53881 ggttatcgct atcagcctga tgttgatggc cagcaagccg ttaacgcaat ttgttaacag
    53941 tcacccgacg atcgttattc tctgcttaag cttcctgttg atgattggct ttagcctggt
    54001 ggcagaaggt ttcggcttcg tcattccgaa aggctacctg tacgctgcca ttggtttctc
    54061 ggtgatgatc gaggcgctca atcagttggc tatctttaac cggcgacgtt ttctttccgc
    54121 taaccagacg ctgcgccagc ggactaccga agcggtaatg cgcctgctta gcgggcaaaa
    54181 agaagatgcg gaactggacg ccgaaaccgc gtccatgctg gtggatcatg gtaaccagca
    54241 gatctttaat ccgcaggaac ggcggatgat tgagcgggta cttaatctta accagcgtac
    54301 cgtcagcagc attatgacgt cgcgccacga tattgagcat atcgatctca acgcgccgga
    54361 agaagaaatc cgccagttgc tggagcgaaa tcagcatacg cggctggtgg ttaccgacgg
    54421 tgatgacgca gaagatttgc tcggtgttgt tcacgttatc gacctgctac aacagtctct
    54481 gcgcggcgaa ccgctcaacc tgcgggtgtt gattcgccag ccgctggtgt tcccggaaac
    54541 cttgccgttg ttacctgccc tggagcagtt ccgtaatgcc cgcacgcact ttgcttttgt
    54601 ggtggatgag tttggctcgg tggaagggat tgtgacatta agtgacgtca ctgaaaccat
    54661 tgtcggtaac ttaccgaacg aagtggaaga gatcgacgcc cgccatgata ttcagaagaa
    54721 tgcggacggt tcctggacgg cgaatggtca tatgccgctg gaagatctgg tgcaatatgt
    54781 gccgctgccg ctggatgaaa aacgtgaata tcacaccatt gccgggctgt tgatggaata
    54841 tttacagcgt attccaaaac ctggcgaaga agttcaggtg ggggattatt tgcttaaaac
    54901 gttgcaggtg gaaagccatc gcgtgcagaa ggtgcagatt attccgctgc ataaggatgg
    54961 cgagttggag tacgaggtgt gatggtatcg gtcattttac ccaatagccg aaaggaataa
    55021 gatgagggaa ccgcgccagc ggcgatttac cgccgggagc ccgggttgcc agggtggtgg
    55081 cgattgagcc accctggcac gttcaccggc ttgagcgttc catagtagcg aggaacatca
    55141 agtgaacgga acagccttcg atcttacata ttcagaatat aatgatgccg ccacgaacgc
    55201 ctgatgcgac gctttacgcg tcttatcagg cctacaggac tggtcacctg gtaacctaca
    55261 tcttctccag caacttcttc acatccttgc cattgcggga atctttattc cgctcggccc
    55321 agtcattcag gcgacgtttc gcttcgtcct gtagatgttt gcgcagtaac tgatccactt
    55381 gcaggctgta attgagttgc tgccagttgc cataaacccg cagcggtact ggcgtttctt
    55441 tcaggaaatc aatcagttta ctttccccgt tccagccacc cacgacccga atatcaaact
    55501 gggtatcgca ggtttgatct gccagattca acatgccttc ccctgtcagc gccagcacag
    55561 gcgattgacc ttgcatgtcg tttaacgtca cgacgccatc cttcaacgtc aaatcggtgg
    55621 taaagcggtc aagccgcgtc acgttatcaa agttttcagc ggccttcaca tcaccgccat
    55681 tacgctctac cgcttgctga atcatctgct ggaagttcat cccttccatg cgcgtgtcgg
    55741 tcatttcgac atgtgcctgt ccttgccagt tgtggcggaa tgcgtcggca tctatgtcag
    55801 caccggagaa atcaccagcc agtgacattt ttccggtcaa cgaaatcgga tagttaaacg
    55861 ccttcagaat ggtaccaatc tcaacgtttt ccagccgtgg ctggaagttt atccgcggat
    55921 ttattgatgt cgcgtccagc gtgcccggca gtgaaacctg tccaccgtta agtttgccct
    55981 gcagttgagt aatttccagc aaacccgact tgttggtcat ttgcgtggca acatctgtaa
    56041 aattcattcc gcgccagcgc acgttactgg cctgcaacaa aatatcagcc gtaaagcctt
    56101 gcagtccctg ataggccggt tcatcaatac gcgaagaaat gaccgggcgc ggcaacgttg
    56161 attggctctg cccctgctgc gcggcaccgt tttcaccatt cgcggtttca ttaagcggga
    56221 tgaggttgtc aagattcagt tgcgggaatt gcagtctcag ctgccattcc ggtttctctg
    56281 tcagcgtgac ctgtgcttgc ccgctcagcg tactgtcatt ggcggtcaag ctgatttggt
    56341 taaatgaaag gcgtttatgt gactcctgcc actgggcctg gaaactcccc tgcccctgaa
    56401 taccttgttt tggtaaatcg gcaccctgca actgccagtt aatttgttca atagccgccg
    56461 ttaaatcatg cggataatca gaagcatcca ccgtaccgtt aagggatatt gtgagatcgc
    56521 gctgatcgcg attaacccgc ccggagaact caaatgagcc acgatgttgg ggatcttgtt
    56581 ccatttgcag gcggatattg cggattgtca cctgctcgtc atcttcatgc tggaacacca
    56641 gcacgctgtc cgccacctta agactggata tatcaaacga ccatccgcga tcgtctgaca
    56701 gatccggcaa ggtattgtcg cgcggtgcaa ccggagcgtc ttcactgcgc accgcttccg
    56761 tctgcggcgt cagttggatc actgcccctt ttagcatcac ctgcttaacg ctcagttgat
    56821 gactcagtag tggtaaaagc gccacgtcca gacgcatgtt gtcggcgcga accagtggct
    56881 ggcttgcgcc ctgggcggtg agagacattc gcccggagag gatactaagc tgcggccaga
    56941 cgtgccaacg cagtggcccg tcgagctgca attgataacc gctacgtgca gcaacttgct
    57001 tgaccatata gtcgcggaaa tcattcggat tcaccagcaa cactaacgca gataacccgg
    57061 ccaccagcac gaccaggagt atcatcagcg tcgtcagaaa tcgtctcatg gtatcctcaa
    57121 tgggcctgaa ttagtcttta tcgattcggc tggctaccgc gccctgctgg ttgcgatatt
    57181 tcgcatcttc acggcggttg taaggtcgcg ccgccgggcc ggaaagcggc tcaaagctca
    57241 gcgcaccaat taacatgccc ggacgcagcg ccagcggcag cttaccggag ttgtagaact
    57301 ccagcacaat gcaaccagac cagcccggat cgatacggtg cgcggtgacg tgcaccatca
    57361 gccccagacg cgccaaagag gaacgcccgt ccagccagcc caccagatcg gctggcagcg
    57421 tcaccgactc cagcgtcacc gccagcgcca gctctcctgg gtgaagatag aacgcctcgc
    57481 cctcgtcgag aacgatctca tcgctcatca cgcggtcaag cgcggcgctc acttcatctt
    57541 tgggaccgct cagatcgata aacgctgccg tgtgaccacg gaaggtacga aatttattgc
    57601 ccaggcgtac atccaccgtc gcgccgttaa tacgctccac tggcggacgt gggttgatcg
    57661 acaaacggcc ttcatcaagc caggcttcaa tatctcggtc acacagacgc atggcatttt
    57721 ctcctttcgc gcatcactcc cttaacgcca attacgtcaa gggcatacca ggttatcact
    57781 gaacggtaca caatttatca agcttattca aagaactgac ttattttcgc tttcaatata
    57841 tcgatcgcga tgcggttttt cccgccgcgc ggcacgataa tgtccgcata ttgtttagaa
    57901 ggctcaatga attgcaggaa catcgggcgc acggtttttt gatattgcgc catcacggaa
    57961 tccattgaac gcccacgctc gttaacgtca cgcttgatgc ggcgcatcag gcagatatcc
    58021 agcggggtat caacgaaaat ggagaagtta agttcgtcac gcaaacgcgc atccgtcagc
    58081 aacaaaatgc cttcgagaat gatgaccttc ttcggctcaa ccgtcaccgt ttctttcata
    58141 cgcgtatgtt caacatagct gtaaaccggc aggtcaattg ccgagccgcg tttcaacgct
    58201 tgtaaatgct caagcagcag gctgtgatcc atcgcgctgg gatggtcgta gttggtctta
    58261 acgcgttctt ccatcgacag atggctttga tctttgtaat agcagtcttc gggaattacg
    58321 ccgatgtgtt catcaccgac ttgctcacgt aattcacgat aaagggtact ggcaataaga
    58381 ctcttgccgg aagccgatgc gccagcgata ccgataatga cgcactgatg agactgatca
    58441 gtcatatatt tagcgacctg attaacctgg atgttaggaa gggggcgacg aagcgccaaa
    58501 cgcggcaatt atagggattt catccgtctg ataccagtcg aatagcgttg ccgcgcgctc
    58561 agagttaatt gttgacaaag aattcccggt ggcaaattac gttgatcagt tttatgtaag
    58621 gtaaaaaatg ttatacgcag ttgcgcaaat tatccgcctt tacgtcactt tatgagcaat
    58681 tcgcatataa aatgtaaaac ttttgtacta gcataaacac agaaacgaat actggcgacc
    58741 tggtcttgcg gataaagcgg taatgagcaa acaatcacag catgtattaa ttgccctgcc
    58801 ccacccgctg cttcacctgg tcagtttagg tttagtctcg tttatcttta cccttttctc
    58861 gcttgagctt tcgcagtttg gcacccaact cgccccactg tggttcccga cgtccatcat
    58921 gatggtggcg ttttatcgcc atgccgggcg catgtggccg ggaattgcgc tgagctgttc
    58981 gctgggaaat atcgccgcat ccatcctgct tttttccacc agctcgctga acatgacctg
    59041 gacgaccatc aatattgttg aagccgtggt cggggcagtg ctgctacgta aattgctgcc
    59101 gtggtataac cccttgcaaa atctggctga ctggctgcgt ctggcactcg gcagcgccat
    59161 tgttccgcct ctgttggggg gtgttctggt tgtcctgctg acgcccggag acgatcctct
    59221 cagggcattt ttgatatggg tactgtcaga atccatcggc gctctggcac tggtgccgct
    59281 gggattgtta tttaaaccac actatctgct gcgccatcgc aacccacggt tgctttttga
    59341 gtcgctgctc acgttagcca tcacactgac gttaagctgg ctttcgatgc tgtatctgcc
    59401 gtggcctttt actttcatta ttgtgctgtt gatgtggagc gccgtgcgcc tgccacgaat
    59461 ggaagccttt ttgatcttcc ttaccacggt gatgatggtg tcgctgatga tggccgcgga
    59521 tccctccctg cttgctacgc cgcgtacgta cctgatgagc catatgccgt ggctaccgtt
    59581 tttgctgatc ctgctgcccg ccaacatcat gaccatggtg atgtatgcct ttcgtgcgga
    59641 acgcaaacac atttccgaaa gcgaaacccg ctttcggaac gcgatggaat attccgctat
    59701 cggtatggcg ttagtgggca ccgagggaca atggctgcaa accaacaaag cgctctgcca
    59761 gtttctcggg tacagtcagg aagagctgcg cggactcacc tttcagcaac tgacctggcc
    59821 ggaggatctc aataaagatc tccaacaggt tgaaaagctg ataagcggtg aaataaacac
    59881 ctattcaatg gaaaaacgct actacaaccg caatggcgat gttgtctggg cgttgcttgc
    59941 cgtctcactg gtgcgccaca cggatggcac gccgctctat tttatcgctc agattgaaga
    60001 cattaacgag ctaaaacgca ccgaacaggt caatcagcaa ctgatggagc gcatcacgct
    60061 ggctaacgaa gcgggcggga ttggcatctg ggagtgggag ttgaagccga atgtttttag
    60121 ctgggataag cggatgttcg agctgtatga aattcctcca catatcaaac cgaactggca
    60181 ggtgtggtac gagtgcgtgc tgccggaaga tcgccagcat gccgaaaaag tgattcgtga
    60241 ttcgttgcaa tcacgctcgc cctttaaact ggaatttcgc attaccgtaa aagacggtat
    60301 tcgccatatc cgcgccctcg ccaaccgggt actgaataaa gaaggcgaag tcgaacgtct
    60361 cctcggcatc aatatggata tgaccgaagt gaaacagctt aacgaggcat tgtttcagga
    60421 aaaagagcgc ctgcacatta cgcttgattc catcggtgaa gccgtggtct gtattgatat
    60481 ggcgatgaaa attaccttta tgaatccagt ggcggagaag atgagcggct ggacgcagga
    60541 agaagcgtta ggtgttccgc tcctgacggt gttgcatatt acttttggcg acaacggacc
    60601 attaatggag aacatttaca gtgccgacac ctcacgttcc gcaatcgaac aagatgtggt
    60661 gttgcactgt cggagcggcg gcagttacga cgtgcattac agtattacgc cgttaagtac
    60721 tctggacggc agcaatattg gttcggttct ggtgattcag gacgtcaccg aatcacgcaa
    60781 aatgctgcgc cagctgagct acagcgcctc ccatgatgca ctgacgcatc tcgccaaccg
    60841 cgccagtttt gagaaacaac tgcgtatcct gctgcaaacg gtaaacagta cgcatcagcg
    60901 acatgccctg gtgtttatcg atcttgatcg ctttaaagcg gtgaatgaca gcgccgggca
    60961 tgcggcgggc gacgctttgc tgcgcgaact ggcgtcgttg atgctgagta tgctgcgctc
    61021 cagtgacgtg ctggcgcggc tcggcggtga tgaatttggt ctgctgctgc cagactgcaa
    61081 tgttgaaagc gctcgtttta tcgctacacg tattatcagt gctgtgaatg actatcactt
    61141 tatatgggaa ggccgggtac atcgggtagg tgccagcgcc gggattacct tgatcgatga
    61201 caacaatcat caggcggcag aagtgatgtc gcaggctgat atcgcctgtt atgcctccaa
    61261 aaatggtgga cggggccggg tgacggttta cgaaccacag caagctgccg cacatagcga
    61321 acgggcggcg atgtcgcttg atgaacagtg gcggatgatt aaagagaatc agttgatgat
    61381 gatcgcccac ggtgtcgctt cgccgcggat cccggaagcg cgtaatttgt ggctgatttc
    61441 gcttaagctc tggagttgcg aaggcgaaat tattgatgaa caaacatttc gtcgtagctt
    61501 cagcgatccg gcacttagcc atgctcttga ccgacgggta ttccacgaat ttttccagca
    61561 ggccgccaaa gcggttgcca gtaaaggcat aagcatcgcc ctcccccttt ccgttgccgg
    61621 tttgagtagc gccacgctgg tgaatgatct gcttgagcag ctggaaaata gccctctacc
    61681 accacggtta ttacatctga ttattccggc tgaagcgatt ttagatcacg cagaaagcgt
    61741 gcaaaaactg cagctggcgg gatgtcggat agtgctcagc caggtgggcc gcgatctgca
    61801 aatcttcaac tcgctgaaag cgaatatggc agattacctg ctacttgatg gtgagttatg
    61861 cgccaacgtg cagggtaatt tgatggatga gatgctgatt acgattattc aggggcacgc
    61921 tcagcgactc gggatgaaaa ccatcgccgg gccagtcgtt ttacccttag tgatggatac
    61981 gctttctggc atcggcgtcg atctgattta tggtgatgtg attgccgatg cccaaccgct
    62041 ggatttgctg gtgaatagca gttatttcgc gattaactga gagagcgttt gcccattgcc
    62101 tgatgcgacg ctaacgcgtc ttatcatgcc tacaaatcgc tcattcccca gaccggataa
    62161 ggcgctcgca ccgcatccgg cgaccaacgt catgcttcgt ctggttgcca gccttccgta
    62221 taccagatat gcaacagcgc ataagaacgc cagggcttcc agcgctcggc ataacggcgg
    62281 atttgtgccg gtgtcattcc cggaaatcgc cgtttaatca gataatcatc cggcagaaaa
    62341 acatctttcg cctgccagcc acgcaaagca aaataattcg ccgtccagcg cccgataccc
    62401 ggaaaagttt gcagcgtttt catcgcctgc tccacatcgc ccggaattgt cattggtaag
    62461 ctgccctcca gcgccgcatt tgccagatga atcagtgcct ctgcccgttt caacggcatg
    62521 cctaacgctt ttaatgcctg cgggtcggct gctgccagcc gctgaggcgt ggggaagcag
    62581 atatactccg gaaaatcatc cagccgttcg ccataaagct gtaccacttt ggcagtcaat
    62641 tttgccgcca tcgccacgct caccagttgg cctaaaatcg cccgcacacc ctgctcaaaa
    62701 gcatcaatac agccgggtaa acgcaatccg ggccgccccg cgcctaactt tcccaacgca
    62761 ccgttaacaa tctgcgggtt acattgcaga tcaagcaggc ggctcatttt cgccagacac
    62821 tctgcggcaa caggttctaa acctgcactt aaatttatgt gcagagtatg gcgggctata
    62881 tccggaatag cagtcactac gccgcgatat tcgcccaccg ccagactacg ggcataataa
    62941 ttgtccgcga ccgtttccac accgctcacc gcacgggcgg cgagaaatcc caacatccac
    63001 gaccagtcat acggcggctg ccagttcagg gtatacatcg catctccttg ttaatccgct
    63061 ttcagcataa acgttattca gacgttacgc tttgctttca tattccggtt gtcgcgacag
    63121 caacatttcg ctaaagtcac gccccttctt caccggcatg gggattaatt cgtgtttatt
    63181 ggttttgatt acggtacagc aaactgttca gtggcggtca tgcgtgacgg taaaccgcaa
    63241 ttactgaaaa tggaaaacga cagcacgctg ctgccttcaa tgctttgcgc gccaacgcgt
    63301 gaatcggtaa gcgaatggct gtaccgccat catgatgttc cggcagacga cgatgaaacg
    63361 caggcgctgt tacgtcgggc gattcgttat aaccgcgaag aagatatcga tgttacggcg
    63421 aaaagcgtgc agttcggtct ttcctcactg gcacagtaca ttgatgatcc agaagaagtg
    63481 tggtttgtga aatcaccaaa atcgttcctc ggtgccagcg gcttaaaacc gcagcaggta
    63541 gcgctgtttg aggatctggt ctgcgcaatg atgttgcaca ttcgccagca ggcgcaggcg
    63601 cagctgccag aagcgattac tcaggcggtg attggtcgcc cgatcaactt ccaggggctg
    63661 ggcggcgatg aagcaaacgc ccaggcgcaa gagattctgg aacgcgcggc gaagcgtgcc
    63721 ggattcaggg acgtggtatt ccagtacgag ccgatcgcgg ctgggctgga ttacgaagcc
    63781 accttgcagg aagaaaaacg ggtgctggtg gtggatatcg gcggtggtac gactgactgt
    63841 tcattgctgc tgatggggcc gcagtggcgt tcgcgtctcg atcgtgaagc cagtctgctg
    63901 ggtcacagtg gttgtcgtat tggcggtaac gatctggata tcgccctggc gtttaaaaac
    63961 ctgatgccac tgctgggtat gggtggcgaa accgaaaaag gcatcgccct gccgatcctg
    64021 ccgtggtgga atgcggttgc catcaacgac gtacctgcgc agagtgattt ctacagtagt
    64081 gccaacggtc gtctgcttaa cgatctggta cgcgatgccc gcgaaccgga aaaagtggcc
    64141 ctgttacaga aagtctggcg tcagcgttta agctatcgcc tggtgcgtag cgcagaagag
    64201 tgcaaaattg ctctttcaag cgtagcggaa acccgcgcct cactgccgtt tatcagcaat
    64261 gaactggcta cgctgattag ccagcgaggg ctggaaagcg ccctcagcca gccactggcg
    64321 cggattctgg aacaggtgca actggccctg gataacgccc aggaaaaacc ggacgttatc
    64381 tacctgaccg gcggtagcgc ccgttctccg cagattaaaa aagcgctggc agaacagttg
    64441 ccgggcattc cgattgcagg cggcgatgac tttggctccg tcaccgccgg gctggcacgc
    64501 tgggcggaag tggtgtttcg ttaattgaaa aatggctcag cagcggctta aatctgcctg
    64561 cgcctgagcc agtttttctg ctgcctgacg taacggttcc atcaacggca tggtccgtgc
    64621 tggagcatga aggcggaatc gctgcaactc tgtcgcgccg actgtcaacg ccgattccag
    64681 ccgatgtcgt ttggcggtca ttttcgccat taccgcttgt ctgtctgctg gcgttatcgc
    64741 ttcattcggc ctgaacacaa aacggcgttc acagttcgct ttccagtcga taaccgcctg
    64801 ggtcagatgg tcgccaaacc ctttcacttg cttaacgcca cgacgggtaa catctgcggc
    64861 tgtttcaata ccaaaagacc gtaacgccgc tttacgcgca gggccaacgc cgggaataga
    64921 ggcaacatca ataaaaaatc cctccagaaa cttctgcttc tgccgttccc ttgcagtgtc
    64981 atgaagtgct gccagagcgc gtttctcttc ttccggtaac ccgagaattt cgtccttcat
    65041 tttttcgagc atcgtccgtt tggcgataaa accttccagg ccgcctacct gctggatctg
    65101 gcggactaaa tggtcatagt ccattttcgc acgattaaat gcctcacggc gttgctgaac
    65161 ttctgctttg tacgctttgc ttgtcaaact gccaataatc cagatagccg ccagcacagg
    65221 aaccaaaata atataacgcg gttctgcctg aaggccgcaa agcagcagcg ataatgctga
    65281 cagtgcgatc tcaagcagaa tgatgtattc gcgccgtaac aggcctaaag gaagcggcct
    65341 gccagtcggt tggaaatgat cgggtaatgg cagttgcaat gctggcggtg ctactgacgc
    65401 catcaccatc gcccagactt ttgccagcac aaaatccccg ccggtggtaa tgacctcttc
    65461 gccgagatca ataaaataga taacaccttg attatccagc gcacaccatg ggcagtcagc
    65521 caaatgaccg ggataaacat gcattgccga aacggtacat ttctttaact gttggcgtag
    65581 agaatccagt gctgataccc acgctttagc cgtcggacgc ccggttgcca cgccactttc
    65641 cgtaaacgcc tgctgaaaca tggcttcaac atcactcggt aacatcgata gcggaatcga
    65701 tcgtggcggc ggttttaaac cacgtcgctg attatctgac gcgtaggcat aacggaaatg
    65761 ggtaatatcc gtctccagcg cattacccgc atcagagata agcggcacac cagaataagg
    65821 atgccggcca ccaaacaaga cgtgaaaaat cagcaacgca aggccaaaat tatcgtgatt
    65881 ttctgtgcgt tcaaagccga caaatgacga caaggtttgt agctctggcg gcgtaaaatg
    65941 cgacacgccg acttcgcata aatgcagtgt gccattggcg ttaatctgaa aggagtcgct
    66001 atcaatcaac accactttgc tgtcgcgacc taccataaaa ctgttctggt ttacgtcacc
    66061 cacgacgtgc ccgtgctcgt gaaccgtagc aaaagatgaa gcaatattgc gcgcaacata
    66121 gagtagaaaa tcccacgcac aatgagggta acgttgacga cgatgtgccg ggctatagat
    66181 catatgaatc ggttctttac cagaaacttt tggcatcata aaaccgataa cttttccgcc
    66241 tcgtccaccg tgaagcgttg cctgcggcca ggcgacataa ttcaacaatt gcgcgtcagc
    66301 tgtcgcagcc ataaaggcaa gtttgtcctg ttttaaggcg ggtggcggcg tgtgataaat
    66361 cttggcgacg ctatcgacaa actcattgat atcataaacc gcgccttcac cgcctttgcc
    66421 cagttcacgg cctggcgtaa cgcactcacc agtagcagta tataaagtgg gtttcatggt
    66481 aaccacagcg ccagtgccaa cgttttatca tcatccgtac gctcgtttac cgccgggctg
    66541 gacaaaaact gctttaacaa ttcaggtaat aaatcgagtt gttcctgcgt tgctgacgcc
    66601 agtccattga aaaacggggt aaagaaaggc acatgaggag aattatccag catatttaac
    66661 gccagccgct gaataccatc cgtaaatgct gcaactttat gcgcacgccc agtgctggtg
    66721 aaagtttcca gtctggaaac ggcatcttcg tcggtaatga agtgcgtcat gttggcatat
    66781 tcacccacca tcggcgtgag aggcaattgc agaccgtgac ccaaatctac caccacgccg
    66841 ccatcgccaa tctgcataat caaggtgccg tcaggcgatg aaatcaatcc cagaaacgtg
    66901 caggcaaaat cgcgaactgc taatgctttg gcttctgcct cggcaaaaag tcgttgatga
    66961 atagtcagta ccatatttgt ggcgaggaca tcattaagcc ccaattcacc gtcctgtact
    67021 ttttgcacca taaacgccat cgcttcattg acggcgagcg tcgcgccttc tcctccctgc
    67081 gaaacgctac ctgcgccatc agcaaggaac atcaccagta atggttgctg gtcgtttaac
    67141 cagacaacct gcatctggca ggcatcctga caaggcaaat cagcactaat gtgcgatgtt
    67201 ccaacagcgg atgcataaac cagacgccag ctcacactga cgtccagcct tttggcgctt
    67261 ccagcacaac ttccgttccc ggcgtggagc gggaaaccga acgcagcgaa ctggataacc
    67321 agctaaacag ttcacggaat tgtaatccct gcagtggcaa aggctgacga acgctgattt
    67381 gtgccagagt cttcatatcc gccccctgaa caccgatgga aaagaaggca aacttcttat
    67441 cttcttcccc ctgaaacact ttgttggcgg cagcctgcca ctcatcggtt ggagcgccat
    67501 cggtgatcag gaaaatccac gggcgataat acgaaatacc atttgcgcgg tactcacgtt
    67561 tcctctcttc aaccatatcc agggctttgg taatggcggc ccccataggc gtatccccct
    67621 gggcaaacag gatgggcggg aaaaagttag ctgcgctggt aaagggttgt tccacatgca
    67681 ccgggccgaa cgtcacaatg ccgagttcca ctctttttaa agccagcgga tcagcaagca
    67741 gttcatcacg aaaggtaacc aatccggcgt taagttcatt aataggtctg ccgctcatcg
    67801 agccagaaac atccagcagc aaaatgcagg ggcaacgtgg ttccgggtta ctggcaaaat
    67861 cgctggtggc aaacgtgatt tgttcgctca taagaaatcc tttcttagag aattatcgag
    67921 gtccggtatc ataccgattg caataatgaa ataagagcaa acgaagaaac tttcagaatt
    67981 aggcccgtcg gcgcagaaaa ttttgctatt cgcctcgcaa gaacggagag ccgtcgattg
    68041 acactaaggg cggagtgaca taatttcagg agtgagggtt agggagaggt ttccccctcc
    68101 ccctggtgtt cttagtaagc ctggaagcta atcactaaga gtatcaccag tatgatgacg
    68161 tgcttcatca taaccctttc cttattaaaa gccctcttct ccgggagagg gtttcccgtt
    68221 tcagcgtccc gctgaaatcg tcggcttacc tcctttcgcc ctgaatgcag tctatcgcta
    68281 acgcgtgaac aaaatagttt catctgtgat tattgcgtaa cgactcgcaa agaatatttg
    68341 ctggcaggat cgcagactac aaagcctgcg gattgacaat cttatcgtga aggcataatt
    68401 tcaggagtga gggtagagcg gggtttcccc cgccctggta gtcttagtaa gcggggaagc
    68461 ttatgactaa gagcaccacg atgatgagta gcttcatcat gaccctttcc ttattaaggc
    68521 cccttcctcg ggaggggctt tcccgtttca gcgtcccgct gaaatcgtcg gcttacctcc
    68581 tttcgccctg tatgcagtct atcgctgact tgcggataaa atagtttcca gtgtgattat
    68641 tgcgtaacgt ctcgcaaaga atatttgctg gcaggatcgc agactgcaaa gcctgcagat
    68701 tgacaatctt atcgtgaagg catactttca ggagtgaggg tagagcgggg tttcccccgc
    68761 cctggtagcc ttagtaagct ggccagctaa tgactaagag caccacgatg atgagtagct
    68821 tcatcatgac cctttcctta tttatggccc cttcctcggg aggggctttc ccgtttcagc
    68881 gtcccgctga aatcggcggc ttacctcctt tcgccatgca agcagtctat cgctaacgcg
    68941 tagataaaat agtttcctgt gttattactg gatgcgtgct cgcaaatgtg cccgtcattc
    69001 agacgattcc cgacagtgtt tcataattcc tccatttttc tcccttattg gctggctaca
    69061 ctagtatcat tccgcgaaac gtttcaggaa gagaaactct taacgatgaa aggcagttat
    69121 aaatcccgtt gggtaatcgt ggtggttatc gccgccatcg ccgcattctg gttctggcaa
    69181 ggccgcaatg actcccagag tgcagcccca ggggcgacga aacaagcgca gcaatcgcca
    69241 gcgggtggtc gccgtggtat gcgttccggc ccattagccc cggttcaggc ggcgaccgcc
    69301 gtagaacagg cagttccgcg ttacctcacc gggcttggca ccattatcgc cgctaatact
    69361 gttacggtgc gcagccgcgt ggacggtcaa ctgatggcgt tacatttcca ggaaggccag
    69421 caggtcaaag caggcgattt actggcagaa attgacccca gccagttcaa agttgcatta
    69481 gcacaaaccc agggccaact ggcaaaagat aaagccacgc ttgctaacgc ccgccgtgat
    69541 ctggcgcgtt atcagcaact ggcaaaaacc aatctcgtct cccgtcagga gctggatgcc
    69601 caacaggcgc tggtcagtga aaccgaaggc accattaagg ctgatgaagc aagcgtcgcc
    69661 agcgcgcagt tgcaactcga ctggagccgc atcaccgcac cagtcgatgg tcgcgttggt
    69721 ctcaagcagg ttgatgttgg taaccaaatc tccagtggtg ataccaccgg aattgtggtg
    69781 atcacccaga cgcatcctat cgatttagtc tttaccctgc cggaaagcga tatcgctact
    69841 gtagtgcagg cgcaaaaagc cggaaaaccg ctggtggtag aagcctggga tcgcaccaat
    69901 tcgaagaaat taagtgaagg cacgctgtta agcctcgata accaaatcga tgccacaacc
    69961 ggtacgatta aagtgaaagc acgctttaat aatcaggatg atgcgctgtt tcccaatcag
    70021 tttgttaacg cgcgcatgtt agtcgacacc gaacaaaacg ccgtagtgat cccaacagcc
    70081 gccctgcaaa tgggcaatga aggccatttt gtctgggtgc tgaatagcga aaacaaggtc
    70141 agcaaacatc tggtgacgcc gggcattcag gacagtcaga aagtggtgat ccgtgcaggt
    70201 atttctgcgg gcgatcgcgt ggtgacagac ggcattgatc gcctgaccga aggggcgaaa
    70261 gtggaagtgg tggaagccca gagcgccacc actccggaag agaaagccac cagccgcgaa
    70321 tacgcgaaaa aaggagcacg ctcctgatgc aggtgttacc cccgagcagc acaggcggcc
    70381 cgtcgcgcct gtttattatg cgtcctgtcg ccaccacgct gctgatggtg gcgatcttac
    70441 tcgccgggat tatcggttat cgcgccctgc ccgtttcggc gctgccggaa gtggactatc
    70501 cgaccattca ggtggtcacg ctctacccag gtgccagccc ggatgtcatg acctctgccg
    70561 ttaccgcgcc gctcgaacgc cagttcgggc agatgtctgg cctgaaacag atgtcgtcgc
    70621 aaagttccgg cggtgcgtca gttatcactt tgcagttcca gctaacatta ccgctcgatg
    70681 tcgccgagca ggaagtgcag gccgcgatta acgctgcgac caacttgttg ccgagcgatc
    70741 tgcctaaccc gccggtttac agcaaagtga acccggcaga tccgccgatc atgacgctcg
    70801 ccgtcacctc aaccgccatg ccgatgacgc aagtggaaga tatggtggaa acccgcgtcg
    70861 cgcagaaaat ctcgcagatt tccggcgtcg gcctggtgac gctttccggc ggtcagcgtc
    70921 cggctgttcg cgtcaaactt aacgctcagg cgattgccgc cctcggcctg accagcgaaa
    70981 ccgtgcgcac cgccattacc ggcgctaacg ttaactcggc aaaaggtagc ctcgacggcc
    71041 cttcccgtgc agtcacgctt tccgcgaacg accagatgca atccgccgaa gagtatcgcc
    71101 agctaatcat cgcctaccag aacggcgcgc caattcgtct gggcgatgtc gcaactgtag
    71161 agcaaggtgc agaaaacagc tggctcggcg cgtgggcgaa caaagaacag gccattgtga
    71221 tgaatgttca gcgccagccc ggtgctaaca ttatctccac cgccgacagc attcggcaga
    71281 tgctgccaca gctcactgag agtctgccga aatcggtgaa ggtgacagtg ctttccgatc
    71341 gcaccaccaa tatccgcgca tccgtcgatg atactcagtt tgaattgatg atggctatcg
    71401 cgctggtagt catgattatc tacctgtttt tgcgcaatat tccggcgacc atcattcccg
    71461 gtgttgctgt accgctgtcg ttaatcggca ctttcgcggt tatggtgttt ctcgattttt
    71521 caatcaataa cctgacactg atggcgttaa ctatcgccac cggattcgtg gtcgatgacg
    71581 ccatcgtggt gatcgaaaac atttcccgct atatcgaaaa aggcgaaaaa ccgttggcgg
    71641 cggcgctcaa gggcgcaggt gaaatcggct ttaccattat ctcgctgacc ttctcactga
    71701 ttgcggtgtt gatcccactg ctgtttatgg gcgatatcgt cgggcgactg ttccgcgaat
    71761 ttgctattac cctggcggta gcgattttga tctcagcggt ggtgtcgctg accctgacac
    71821 cgatgatgtg cgcgcggatg ctcagccagg agtcgttgcg taaacagaac cgcttctccc
    71881 gtgcctcgga aaaaatgttc gacaggataa tcgccgccta tggtcgtgga ctggcgaaag
    71941 tgctgaatca tccgtggctg accttaagcg tggcactcag cacgctgctg cttagcgtgc
    72001 tgctgtgggt gttcattccg aaaggtttct tcccggtaca ggacaatggc attattcagg
    72061 gcactttgca ggcaccgcaa tccagctcct ttaccaatat ggcccagcga caacgccagg
    72121 tcgcggacgt gattttgcag gatccggcag tgcaaagcct gacctcattt gttggcgttg
    72181 atggcactaa cccgtcgctg aatagtgcac gtttacaaat caacctcaaa ccgttggatg
    72241 aacgtgatga tcgggtgcaa aaagtcatcg cccgtctgca aacggcggta gataaagtgc
    72301 cgggcgtcga tctcttcctg caaccaacgc aggatctgac tattgatact caggtcagcc
    72361 gcacccagta ccagtttacc ttgcaggcca cctcactgga tgcgctcagt acctgggtgc
    72421 cacagttgat ggaaaaactc cagcaactgc cacagctttc tgatgtctcc agcgactggc
    72481 aggaccaagg gctggtggcg tatgtcaatg ttgatcgcga cagcgccagc cgtctgggga
    72541 tcagcatggc ggatgtcgat aacgccctgt acaacgcgtt tggtcagcga ctgatttcca
    72601 ctatttatac tcaggctaac cagtatcgcg tagtgctgga gcacaacacc gaaattaccc
    72661 cgggcctcgc ggcgctggat accattcgcc tgaccagtag cgacggcggc gtggtgccgt
    72721 taagctcaat tgccaaagtt gaacagcgtt ttgcgccgct ttccatcaac catctggatc
    72781 agttcccggt aacgaccatc tcctttaacg tgccggataa ctattcgctg ggcgatgcgg
    72841 tgcaggagat tatggacacc gaaaagacgc tgaatctgcc ggtggatatc accacgcaat
    72901 tccagggcag caccctcgcc ttccagtcgg cgctgggcag cactgtctgg ctgattgtcg
    72961 cggcggtggt ggcgatgtat atcgtgctcg gcattctgta cgagagcttt attcacccga
    73021 tcaccattct ctcgacgcta cccaccgcag gggtcggcgc gctgctggca ttaatgattg
    73081 ccggtagcga actggatgtg attgcgatta tcggcattat tttgctgatc ggtattgtga
    73141 agaagaacgc catcatgatg atcgacttcg cgctggctgc tgagcgcgag caaggcatgt
    73201 cgccgcgcga tgcaatctac caggcttgtc tgttgcgttt tcgtccgatc ctgatgacca
    73261 ccctggcggc tctgcttggc gcgctgccgc tgatgttgag taccggggtc ggcgcggaac
    73321 tgcgtcgtcc gttaggtatc ggcatggtcg gcggtctgat tgtcagccag gtgctgacgc
    73381 tgttcaccac gccggtgatt tatctgctgt tcgaccgcct ggcattgtgg accaaaagtc
    73441 gctttgctcg ccatgaagag gaggcgtaag tgaagttttt tgccctcttc atttaccgcc
    73501 cggtggcgac gattttactg tcggtcgcca ttaccctgtg cggcatactg ggcttccgta
    73561 tgctgccggt cgccccgctg ccgcaggtcg attttccggt gattatggtc agcgcctcgc
    73621 tgcccggtgc gtcaccagaa acaatggcgt cttccgttgc cacgccgctg gagcgctcgc
    73681 ttgggcgcat tgccggggtc agcgaaatga cctccagcag ttcgctcggc agcacacgta
    73741 ttattttgca gtttgatttt gaccgggaca tcaacggcgc agcgcgtgat gtgcaggcgg
    73801 cgatcaacgc tgcacaaagt ttgctgccca gtgggatgcc cagccgtccg acctatcgca
    73861 aagcgaaccc gtcggatgcg ccaattatga tcctcacgct gacatccgat acttattcgc
    73921 agggtgaact gtacgatttc gcctcgacgc agctggctcc gacgatttcg caaatcgacg
    73981 gtgttggtga tgtcgatgtc ggaggcagct cactgcccgc cgtacgcgtc gggctgaatc
    74041 cgcaggcgct gtttaatcag ggcgtgtcgc tggacgacgt acgcaccgcc atcagcaatg
    74101 ccaacgtgcg taaaccgcag ggcgcgctgg aagatggcac tcaccgctgg cagatccaga
    74161 ccaatgatga gctaaaaacc gccgctgaat atcagccgtt gattattcac tacaacaacg
    74221 gcggcgcggt tcgtctgggc gatgtggcga cggtgaccga ctcagtgcag gatgtgcgca
    74281 acgccgggat gaccaacgcc aaaccggcta ttttactgat gatccgcaaa ctgccggaag
    74341 ccaatattat ccagacggtt gacagcatcc gggcaaaatt accggagttg caggaaacca
    74401 ttccggcggc gattgatctg caaattgccc aggatcgctc ccccaccatt cgcgcctcgc
    74461 tggaagaagt cgagcaaacg ctgattatct cggtggcgct ggtgattctg gtggtgtttt
    74521 tattcctgcg ctcgggtcgc gccactatta ttcccgccgt ttcggtgccg gtttcgctga
    74581 ttggtacgtt tgcggcgatg tacctgtgcg gattcagtct caataacctt tcgttaatgg
    74641 cgctcaccat cgctactggt ttcgtggtgg atgacgccat cgtggtgctg gaaaacattg
    74701 cacgtcatct ggaagcggga atgaaaccgt tgcaagccgc actgcaaggt actcgcgaag
    74761 tcggttttac ggtgctgtcg atgagtctgt cactggtggc ggtgttcctg ccgctgctgt
    74821 tgatgggcgg attgccgggc cgactgttac gcgagtttgc cgtgacgctt tctgtcgcca
    74881 ttggtatttc gttgctggtt tctctgacat taacgccaat gatgtgtggc tggatgctga
    74941 aagccagcaa gccgcgcgag caaaagcgac tgcgtggttt tggtcgcatg ttggtagccc
    75001 tgcaacaagg ctacggcaag tcactgaaat gggtgctcaa tcatacccgt ctggtgggcg
    75061 tggtgctgct tggcaccatt gcgctgaata tctggctgta tatctcgatc ccgaaaacct
    75121 tcttcccgga gcaggacact ggcgtgttga tgggcgggat tcaggcggat cagagtattt
    75181 cgtttcaggc gatgcgcggt aagttgcagg atttcatgaa aattatccgt gacgatccgg
    75241 cagtggataa tgtcaccggc tttacaggcg gttcgcgagt gaacagcggg atgatgttta
    75301 tcaccctcaa gccacgcgac gaacgcagcg aaacggcgca gcaaattatc gaccgtctgc
    75361 gcgtaaaact ggcgaaagaa ccgggggcga atctgttcct gatggcggta caggatattc
    75421 gcgttggtgg gcgtcagtcg aacgccagct accagtacac gttgttatcc gacgacctgg
    75481 cggcactgcg cgaatgggag ccgaaaatcc gcaaaaaact ggcgacgttg ccggaactgg
    75541 cggacgtgaa ctccgatcag caggataacg gcgcggagat gaatctggtt tacgaccgcg
    75601 acaccatggc acggctggga atcgacgtgc aggccgccaa cagcctgtta aataacgcct
    75661 tcggtcagcg gcaaatctcg accatttacc agccgatgaa ccagtataaa gtggtgatgg
    75721 aagtggatcc gcgctatacc caggacatca gtgcgctgga aaaaatgttc gttatcaata
    75781 acgaaggcaa agcgatcccg ctgtcgtatt tcgctaaatg gcaaccggcg aacgctccgc
    75841 tgtcggtgaa tcatcaggga ttatcggcgg cctcgaccat ctcgttcaac ctgccgaccg
    75901 gaaaatcgct ctcggacgcc agtgcggcga tcgatcgcgc aatgacccag cttggtgtgc
    75961 cttcgacggt gcgcggcagt tttgccggaa cagcgcaggt gttccaggag acgatgaact
    76021 cacaggtgat cctgattatc gccgccatcg ccacggtgta tatcgtgctg ggtatccttt
    76081 acgagagtta cgtacaccca ctgacgattc tctccaccct gccctcggcg ggcgttggtg
    76141 cgctgctggc gctggagctg ttcaatgccc cgttcagcct aatagccctg atagggatca
    76201 tgctattaat cggcatcgta aagaaaaacg ccattatgat ggtcgatttt gcgctcgaag
    76261 cccaacggca cggtaacctg acgccgcagg aagctatttt ccaggcctgt ctgctgcgtt
    76321 ttcgcccgat tatgatgact accctggcgg cgctgtttgg cgcgctgccg ctggtgttgt
    76381 cgggcggtga cggctcggag ctgcggcacc ccctggggat caccattgtc ggtggactgg
    76441 taatgagcca gcttctgacg ctgtatacca cgccggtggt gtatctcttt ttcgaccgtc
    76501 tgcggctgcg tttttcgcgt aaacctaaac aaacggtaac cgagtaaatg acagaacttc
    76561 ccgacagcac ccgctggcaa ttgtggattg tggctttcgg cttctttatg cagtcgctgg
    76621 acaccaccat cgttaacacc gcccttccct caatggcgca aagcctcggg gaaagtccgt
    76681 tgcatatgca catggtcatt gtctcttatg tgctgaccgt ggcggtgatg ctgcccgcca
    76741 gcggctggct ggcggacaaa gtcggcgtgc gcaatatttt ctttaccgcc atcgtgctgt
    76801 ttactctcgg ttcactgttt tgcgcgcttt ccggcacgct gaacgaactg ttgctggcac
    76861 gcgcgttaca gggcgttggc ggtgcgatga tggtgccggt cggcaggttg acggtgatga
    76921 aaatcgtacc gcgcgagcaa tatatggcgg cgatgacctt tgtcacgtta cccggtcagg
    76981 tcggtccgct gctcggtccg gcgctcggcg gtctgctggt ggagtacgcg tcgtggcact
    77041 ggatcttttt gatcaacatt ccggtgggga ttatcggtgc gatcgccaca ttgctgttaa
    77101 tgccgaacta caccatgcag acgcggcgct ttgatctttc cggcttttta ttgctggcgg
    77161 ttggcatggc tgtattaacc ctggcgctgg acggcagtaa aggtacaggt ttatcgccgc
    77221 tggcgattgc tggcctggtc gcagttggcg tggtggcact ggtgctttat ctgctgcacg
    77281 ccagaaataa caaccgcgcc ctgttcagtc tgaaactgtt ccgcactcgt accttttcgc
    77341 tgggcctggc ggggagcttt gccggacgta ttggcagtgg catgttgccc tttatgacac
    77401 cggttttcct gcaaattggc ctcggtttct cgccgtttca tgccggactg atgatgatcc
    77461 cgatggtact tggcagcatg ggaatgaagc gaattgtggt tcaggtggtg aatcgctttg
    77521 gttatcgtcg ggtactggta gcgaccacgc tgggtctgtc gctgatcacc ctgttgttta
    77581 tgactaccgc tctgttgggc tggtactacg ttttgccgtt cgtcctgttt ttacaaggga
    77641 tggtcaactc gacccgtttc tcctccatga acaccctgac gctgaaagat ctcccggaca
    77701 atctggcgag cagcggcaac agtctgctgt cgatgattat gcaattgtcg atgagtatcg
    77761 gcgtcaccat cgccgggctg ttgctgggac tttttggttc gcagcatgtc agcgtcgaca
    77821 gcggcaccac acaaaccgtc tttatgtaca cctggcttag catggcgttt atcatcgccc
    77881 ttccggcgtt catctttgcc agagtgccga acgatacgca tcaaaatgta gctatttcgc
    77941 ggcgaaaaag gagcgcgcaa tgaagttctg gcgacctggt attaccggca aactgtttct
    78001 ggcgattttc gccacctgca ttgttttgct gatcagtatg cactgggcgg tgcgtatcag
    78061 ttttgagcgt ggctttattg attacatcaa gcatggtaat gaacagcgat tacaactgtt
    78121 aagtgatgcg cttggcgagc agtatgcgca gcatggcaac tggcgcttcc tgcgcaacaa
    78181 tgatcgcttt gtctttcaga tcctgcgttc atttgaacac gataattcgg aagataaacc
    78241 cggcccgggt atgccaccgc acggctggcg tacccagttc tgggtggttg atcaaaacaa
    78301 caaagtgctg gttggtccgc gagcgccgat tccacctgac ggtacacggc gacccattct
    78361 ggtcaacggt gcggaagttg gcgcggtgat cgcctccccc gttgagcggt taacgcgcaa
    78421 tactgatatc aatttcgata aacaacagcg gcaaaccagc tggttgattg tcgccctggc
    78481 aacgttactc gcggcacttg ccacttttct gctggcgcgc ggtttactgg caccggtaaa
    78541 acgacttgtc gatggcacgc acaaactggc ggcgggcgat ttcactaccc gcgtaacgcc
    78601 caccagtgaa gatgaactgg gcaaactggc gcaagacttc aaccagcttg ccagcacact
    78661 ggagaaaaac cagcaaatgc ggcgcgattt tatggccgat atttctcacg aactgcgtac
    78721 gccattagcg gtgctgcgcg gtgaactgga agctattcag gatggcgtgc gcaaattcac
    78781 gccggagacg gtggcgtctt tacaggcgga ggtcggtaca ctgaccaaac tggttgacga
    78841 tctccatcag ttgtcgatgt ctgatgaagg cgccctcgcc tatcaaaaag caccggtaga
    78901 tttgatccca ctgctggaag tggcgggcgg cgcatttcgc gaacgattcg ccagccgtgg
    78961 cctgaaactg caattttccc tgccagacag tattaccgta tttggcgatc gcgaccgttt
    79021 aatgcagtta ttcaataatt tacttgaaaa cagcctgcgc tacactgaca gcggcggcag
    79081 cctgaaaatc tctgccgagc agcacgacaa aacggtgcgc ctgacctttg ccgacagtgc
    79141 gccaggtgtc agtgacgagc agctacaaaa attatttgaa cgtttttatc gcaccgaagg
    79201 ttcccgcaac cgcgccagcg gtggttccgg gctggggctg gcgatttgcc tgaacattgt
    79261 tgaagcacat aatggccgca ttattgctgc ccattcgcct tttggcgggg taagcattac
    79321 agtagagtta ccgctggaac gggatttaca gagagaagta tgaccgagtt accaatcgac
    79381 gaaaacacac cgcgtatttt gatcgtggaa gatgaaccga agctggggca gttgctcatt
    79441 gattatctgc gtgctgcgag ctatgcgccg acgcttatca gccacggcga tcaggtactg
    79501 ccgtatgtgc gccagacacc accggatctg atcctgttgg atctgatgct ccctggcacc
    79561 gatggcttga cgctgtgccg ggaaattcgt cgtttttctg acattcctat cgtgatggtg
    79621 acggcaaaaa tcgaagagat cgaccgcctg ctcggactgg agattggcgc agatgactac
    79681 atctgcaagc cgtacagccc acgggaagtg gtagcgcgcg tcaaaaccat tttgcgccgt
    79741 tgcaaaccgc agcgcgaatt gcagcaacag gatgccgaaa gcccgttgat tatcgacgaa
    79801 ggtcgttttc aggcttcatg gcgcggtaaa atgcttgacc tgacgcctgc ggaatttcgt
    79861 ctgttgaaaa cgctctctca tgaaccagga aaagtgttct cccgcgagca attgctcaat
    79921 catctttatg acgactaccg cgtagtaacc gaccgcacca ttgacagcca cattaaaaac
    79981 ctgcgccgca agctggaatc tctcgacgcc gaacagtcat ttatccgcgc cgtttacggc
    80041 gttggttacc gctgggaagc cgacgcctgc cgcatcgttt agttttagcg acatgacttt
    80101 gttagccgga gatggcgttc agaccagatc cggcaacatt atcccacgca tggtcagcaa
    80161 actga
//
LOCUS       DAZPDU010000002        67003 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZPDU010000002 DAZPDU010000000
VERSION     DAZPDU010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67003
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.002"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            33..665
                     /locus_tag="ACHURS_000080"
                     /pseudo
     CDS             33..665
                     /locus_tag="ACHURS_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            768..1270
                     /locus_tag="ACHURS_000081"
                     /pseudo
     CDS             768..1270
                     /locus_tag="ACHURS_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
     gene            complement(1337..2794)
                     /gene="nuoN"
                     /locus_tag="ACHURS_000082"
     CDS             complement(1337..2794)
                     /gene="nuoN"
                     /locus_tag="ACHURS_000082"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFR1235923.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPMGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLYSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSASGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2801..4330)
                     /gene="nuoM"
                     /locus_tag="ACHURS_000083"
     CDS             complement(2801..4330)
                     /gene="nuoM"
                     /locus_tag="ACHURS_000083"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFR1235924.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRLGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     AEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     EIASQELPGMSLRELFMILLLVVLLVLQGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4494..6335)
                     /gene="nuoL"
                     /locus_tag="ACHURS_000084"
     CDS             complement(4494..6335)
                     /gene="nuoL"
                     /locus_tag="ACHURS_000084"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFR1235925.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEILHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAIVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVRPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6332..6634)
                     /gene="nuoK"
                     /locus_tag="ACHURS_000085"
     CDS             complement(6332..6634)
                     /gene="nuoK"
                     /locus_tag="ACHURS_000085"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFR1235926.1"
                     /translation="MIPLQHGLILAAILFILGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6631..7185)
                     /gene="nuoJ"
                     /locus_tag="ACHURS_000086"
     CDS             complement(6631..7185)
                     /gene="nuoJ"
                     /locus_tag="ACHURS_000086"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFR1235927.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7197..7739)
                     /gene="nuoI"
                     /locus_tag="ACHURS_000087"
     CDS             complement(7197..7739)
                     /gene="nuoI"
                     /locus_tag="ACHURS_000087"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFR1235928.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPIYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7754..8731)
                     /gene="nuoH"
                     /locus_tag="ACHURS_000088"
     CDS             complement(7754..8731)
                     /gene="nuoH"
                     /locus_tag="ACHURS_000088"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFR1235929.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8728..11454)
                     /gene="nuoG"
                     /locus_tag="ACHURS_000089"
     CDS             complement(8728..11454)
                     /gene="nuoG"
                     /locus_tag="ACHURS_000089"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFR1235930.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     HVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11507..12844)
                     /gene="nuoF"
                     /locus_tag="ACHURS_000090"
     CDS             complement(11507..12844)
                     /gene="nuoF"
                     /locus_tag="ACHURS_000090"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFR1235931.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12841..13341)
                     /gene="nuoE"
                     /locus_tag="ACHURS_000091"
     CDS             complement(12841..13341)
                     /gene="nuoE"
                     /locus_tag="ACHURS_000091"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFR1235932.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13344..15146)
                     /gene="nuoC"
                     /locus_tag="ACHURS_000092"
     CDS             complement(13344..15146)
                     /gene="nuoC"
                     /locus_tag="ACHURS_000092"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFR1235933.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15240..15902)
                     /gene="nuoB"
                     /locus_tag="ACHURS_000093"
     CDS             complement(15240..15902)
                     /gene="nuoB"
                     /locus_tag="ACHURS_000093"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFR1235934.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15918..16361)
                     /gene="nuoA"
                     /locus_tag="ACHURS_000094"
     CDS             complement(15918..16361)
                     /gene="nuoA"
                     /locus_tag="ACHURS_000094"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFR1235935.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16990..17928)
                     /gene="lrhA"
                     /locus_tag="ACHURS_000095"
     CDS             complement(16990..17928)
                     /gene="lrhA"
                     /locus_tag="ACHURS_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFR1235936.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPIEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            18848..20065
                     /gene="alaA"
                     /locus_tag="ACHURS_000096"
     CDS             18848..20065
                     /gene="alaA"
                     /locus_tag="ACHURS_000096"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFR1235937.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20149..20748
                     /gene="yfbR"
                     /locus_tag="ACHURS_000097"
     CDS             20149..20748
                     /gene="yfbR"
                     /locus_tag="ACHURS_000097"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFR1235938.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(20807..22639)
                     /locus_tag="ACHURS_000098"
     CDS             complement(20807..22639)
                     /locus_tag="ACHURS_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFR1235939.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(22726..23374)
                     /gene="hxpA"
                     /locus_tag="ACHURS_000099"
                     /pseudo
     CDS             complement(22726..23374)
                     /gene="hxpA"
                     /locus_tag="ACHURS_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416796.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
     gene            complement(23385..23879)
                     /locus_tag="ACHURS_000100"
     CDS             complement(23385..23879)
                     /locus_tag="ACHURS_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFR1235940.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(23962..24417)
                     /gene="yfbV"
                     /locus_tag="ACHURS_000101"
     CDS             complement(23962..24417)
                     /gene="yfbV"
                     /locus_tag="ACHURS_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFR1235941.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            24755..25957
                     /gene="ackA"
                     /locus_tag="ACHURS_000102"
     CDS             24755..25957
                     /gene="ackA"
                     /locus_tag="ACHURS_000102"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFR1235942.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26032..28176
                     /gene="pta"
                     /locus_tag="ACHURS_000103"
     CDS             26032..28176
                     /gene="pta"
                     /locus_tag="ACHURS_000103"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFR1235943.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28366..29886
                     /gene="yfcC"
                     /locus_tag="ACHURS_000104"
     CDS             28366..29886
                     /gene="yfcC"
                     /locus_tag="ACHURS_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFR1235944.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATMIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(29919..30461)
                     /gene="yfcD"
                     /locus_tag="ACHURS_000105"
     CDS             complement(29919..30461)
                     /gene="yfcD"
                     /locus_tag="ACHURS_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFR1235945.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30519..31070)
                     /gene="yfcE"
                     /locus_tag="ACHURS_000106"
     CDS             complement(30519..31070)
                     /gene="yfcE"
                     /locus_tag="ACHURS_000106"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFR1235946.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31126..31770)
                     /gene="yfcF"
                     /locus_tag="ACHURS_000107"
     CDS             complement(31126..31770)
                     /gene="yfcF"
                     /locus_tag="ACHURS_000107"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFR1235947.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLKPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RRIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            31906..32553
                     /gene="yfcG"
                     /locus_tag="ACHURS_000108"
     CDS             31906..32553
                     /gene="yfcG"
                     /locus_tag="ACHURS_000108"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFR1235948.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            32610..32972
                     /gene="folX"
                     /locus_tag="ACHURS_000109"
     CDS             32610..32972
                     /gene="folX"
                     /locus_tag="ACHURS_000109"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFR1235949.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            32993..33886
                     /locus_tag="ACHURS_000110"
     CDS             32993..33886
                     /locus_tag="ACHURS_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFR1235950.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNDLRG
                     PFNMVSPYPVRNEQFAHALGQALHRPAILRVPATAIRLLMGESSILVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(33934..34824)
                     /gene="rpnB"
                     /locus_tag="ACHURS_000111"
     CDS             complement(33934..34824)
                     /gene="rpnB"
                     /locus_tag="ACHURS_000111"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFR1235951.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLESNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKLEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(35021..35794)
                     /gene="hisP"
                     /locus_tag="ACHURS_000112"
     CDS             complement(35021..35794)
                     /gene="hisP"
                     /locus_tag="ACHURS_000112"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFR1235952.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(35802..36518)
                     /locus_tag="ACHURS_000113"
     CDS             complement(35802..36518)
                     /locus_tag="ACHURS_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1235953.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36515..37201)
                     /gene="hisQ"
                     /locus_tag="ACHURS_000114"
     CDS             complement(36515..37201)
                     /gene="hisQ"
                     /locus_tag="ACHURS_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFR1235954.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37291..38073)
                     /gene="hisJ"
                     /locus_tag="ACHURS_000115"
     CDS             complement(37291..38073)
                     /gene="hisJ"
                     /locus_tag="ACHURS_000115"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFR1235955.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38294..39076)
                     /gene="argT"
                     /locus_tag="ACHURS_000116"
     CDS             complement(38294..39076)
                     /gene="argT"
                     /locus_tag="ACHURS_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFR1235956.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKAFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39342..39911)
                     /gene="ubiX"
                     /locus_tag="ACHURS_000117"
     CDS             complement(39342..39911)
                     /gene="ubiX"
                     /locus_tag="ACHURS_000117"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFR1235957.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40006..41523)
                     /gene="purF"
                     /locus_tag="ACHURS_000118"
     CDS             complement(40006..41523)
                     /gene="purF"
                     /locus_tag="ACHURS_000118"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFR1235958.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41560..42048)
                     /gene="cvpA"
                     /locus_tag="ACHURS_000119"
     CDS             complement(41560..42048)
                     /gene="cvpA"
                     /locus_tag="ACHURS_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFR1235959.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42307..42969)
                     /gene="dedD"
                     /locus_tag="ACHURS_000120"
     CDS             complement(42307..42969)
                     /gene="dedD"
                     /locus_tag="ACHURS_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFR1235960.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(42959..44227)
                     /gene="folC"
                     /locus_tag="ACHURS_000121"
     CDS             complement(42959..44227)
                     /gene="folC"
                     /locus_tag="ACHURS_000121"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFR1235961.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSAKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44297..45211)
                     /gene="accD"
                     /locus_tag="ACHURS_000122"
     CDS             complement(44297..45211)
                     /gene="accD"
                     /locus_tag="ACHURS_000122"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFR1235962.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVHRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45367..46026)
                     /locus_tag="ACHURS_000123"
     CDS             complement(45367..46026)
                     /locus_tag="ACHURS_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR1235963.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(46109..46921)
                     /gene="truA"
                     /locus_tag="ACHURS_000124"
     CDS             complement(46109..46921)
                     /gene="truA"
                     /locus_tag="ACHURS_000124"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFR1235964.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPERWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(46921..47934)
                     /locus_tag="ACHURS_000125"
     CDS             complement(46921..47934)
                     /locus_tag="ACHURS_000125"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFR1235965.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48000..49136)
                     /gene="pdxB"
                     /locus_tag="ACHURS_000126"
     CDS             complement(48000..49136)
                     /gene="pdxB"
                     /locus_tag="ACHURS_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFR1235966.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            49235..50230
                     /gene="flk"
                     /locus_tag="ACHURS_000127"
     CDS             49235..50230
                     /gene="flk"
                     /locus_tag="ACHURS_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFR1235967.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVNDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(50227..51405)
                     /locus_tag="ACHURS_000128"
     CDS             complement(50227..51405)
                     /locus_tag="ACHURS_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1235968.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGFI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTEFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(51680..52900)
                     /gene="fabB"
                     /locus_tag="ACHURS_000129"
     CDS             complement(51680..52900)
                     /gene="fabB"
                     /locus_tag="ACHURS_000129"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFR1235969.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            53059..55065
                     /gene="mnmC"
                     /locus_tag="ACHURS_000130"
     CDS             53059..55065
                     /gene="mnmC"
                     /locus_tag="ACHURS_000130"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFR1235970.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLNEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLTALNPNRLWVRKLLKGKAVKAG"
     gene            complement(55186..55464)
                     /locus_tag="ACHURS_000131"
     CDS             complement(55186..55464)
                     /locus_tag="ACHURS_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFR1235971.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(55498..56046)
                     /gene="epmC"
                     /locus_tag="ACHURS_000132"
     CDS             complement(55498..56046)
                     /gene="epmC"
                     /locus_tag="ACHURS_000132"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFR1235972.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56046..56855)
                     /locus_tag="ACHURS_000133"
     CDS             complement(56046..56855)
                     /locus_tag="ACHURS_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFR1235973.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(56855..57679)
                     /gene="mepA"
                     /locus_tag="ACHURS_000134"
     CDS             complement(56855..57679)
                     /gene="mepA"
                     /locus_tag="ACHURS_000134"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFR1235974.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFAPLKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(57683..58768)
                     /gene="aroC"
                     /locus_tag="ACHURS_000135"
     CDS             complement(57683..58768)
                     /gene="aroC"
                     /locus_tag="ACHURS_000135"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFR1235975.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(58803..59735)
                     /gene="prmB"
                     /locus_tag="ACHURS_000136"
     CDS             complement(58803..59735)
                     /gene="prmB"
                     /locus_tag="ACHURS_000136"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFR1235976.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            59901..60452
                     /gene="smrB"
                     /locus_tag="ACHURS_000137"
     CDS             59901..60452
                     /gene="smrB"
                     /locus_tag="ACHURS_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFR1235977.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60574..61446)
                     /locus_tag="ACHURS_000138"
     CDS             complement(60574..61446)
                     /locus_tag="ACHURS_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFR1235978.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVTYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVTSYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNEL"
     gene            complement(61433..61957)
                     /locus_tag="ACHURS_000139"
     CDS             complement(61433..61957)
                     /locus_tag="ACHURS_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1235979.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(61954..62424)
                     /locus_tag="ACHURS_000140"
     CDS             complement(61954..62424)
                     /locus_tag="ACHURS_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1235980.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTLSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLMVNETDLPVLTAVPVKKSGVVLPEADFEAWATLQVDYQ"
     gene            complement(62421..62969)
                     /locus_tag="ACHURS_000141"
     CDS             complement(62421..62969)
                     /locus_tag="ACHURS_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1235981.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINGVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(62944..63695)
                     /locus_tag="ACHURS_000142"
                     /pseudo
     CDS             complement(62944..63695)
                     /locus_tag="ACHURS_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(63715..66357)
                     /gene="yfcU"
                     /locus_tag="ACHURS_000143"
                     /pseudo
     CDS             complement(63715..66357)
                     /gene="yfcU"
                     /locus_tag="ACHURS_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            complement(66439..67002)
                     /locus_tag="ACHURS_000144"
     CDS             complement(66439..67002)
                     /locus_tag="ACHURS_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1235982.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTCAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        16669 a        18172 c        16700 g        15462 t
ORIGIN      
        1 tgataaaata acgtctttta ttgataattt atatatggaa aaaaattcct tttgttatat
       61 tgtttctgat ttcactccct ctgattattt ttaccaaccc tgggtcactg cattatcgcc
      121 aacgccccga cacgtcagtc ctgagcaatt agaaaaaaca gttcgttatc ttacacaaac
      181 tgtgcatcca cgtaatgccg acaatattga taatctgaat aggtccgtag agtatataaa
      241 agagttcttt gtcagtagcg gtgccagagt taccgcgcag agcgtccccg attggctatt
      301 acgactctgc gcctggtagc caggattacc cttacccgca atgtcctggc tttatcccga
      361 tcggggggac tttattgcct ggtcggcaga atacaggata tcaacgccgt tcgtcaggta
      421 aaaacggcat tgttatcatc ccgggattta tctgtttatt ctatgaatgc cccatggttt
      481 attcccggta ttgatttctc tgaccacctg aattattggc aacacgatat tcccgccgta
      541 atgattactg acaccgcttt ttatcgtaat aaacaatacc acttgcccgg tgatacagca
      601 gacagattga attatcagaa aatggctcag atggtgttat aaccttgtta cacaacagta
      661 aataataaaa atttctcgag gcgggtccag aaacagcgac atgtacattt tattttccct
      721 cgtctgtttt aagatgcgaa acataaaatt caatggagga aaaataaatg ggaatgattg
      781 gttattttgc agaaattgac tcagaaaaga ttaaccaact gcttgagtct actgaaaaac
      841 ctttgatgga taatatacat gacacgcttt caggattacg ccgactggat attgataaac
      901 gctgggattt tttacacttt gggctgacag gcacccctgc ttttgatccc gcgaagaatg
      961 atccgctgag tcgtgccgtg cttggtgaac acagcctcga agatggcata gatggcttcc
     1021 tgggattgac ctggaatcag gagttagccg cgacaatcga ccgactggaa tcactggatc
     1081 gaagtgagct gcgtaaacag ttttccatta agcgactcaa tgaaatggag atttatccgg
     1141 gagtgacctt ttcagaagag ttagaggggc aactctttgc ttcgatcatg ctgatatgga
     1201 aaagctgatt agcgcgtatc gacgtatgct gcgacaaggg aatcatgcgc tgacggttat
     1261 tgttgggtga tggtgtcagg gcaccaaaaa aaaccgccga acatgtcggc ggctttctga
     1321 cttacaaact gatagcttac atcagcggca ttgctaaccg cacaatgcta atcagcggtt
     1381 gtggccatac acccagcacc agtaccaaca gtgcagagat cagcaccacg ataccgcctg
     1441 cgctgtactg ccagtttgac ggtgcatcgc gacccggttg ttccggggcg tgcaggtaca
     1501 ggctcaccgc cacgcgcagg tagtagtaaa ggccgcttgc cgaaccgaca accacggcgc
     1561 ccaccagcca ccacaagtgt gcctggacac cgaccgccag cacgtagaac ttaccaataa
     1621 agcccagcgt catcggaata ccggccagag acagcatcat caccgtcatc accgccgcga
     1681 ggatcgggcg atgccagaac agtccgcggt aagagtacag ggaatcagca tccgggccac
     1741 gatacgggct ggacatcagg ctgaccacgc cgaacgcgcc gaggctgcta aacagataac
     1801 cggccaggta aacccctacc gcttccatcg acatctcgcc agtttgcagc gcaatcagcg
     1861 ccaccagcag atagccgagg tgagagatag atgagtaacc gagcagacgt ttgatattgg
     1921 tctggctcag cgccatcagg ttaccgaaga tgatggaggc aaaggcgata atcgccagca
     1981 ccacgcgaat cgcttcgctg tcacccatcg gtgcgtagag gaacagacgc atcaccacac
     2041 cgaagatagc gattttgctc gccgtcgcca ggaaagtgga aaccggcgca ggcgcgccct
     2101 ggtatacgtc tggcgtccac aggtggaacg gcaccagaga gagtttgaag ccgaggccaa
     2161 caatcatcag gccgaaacct gccagcaaca gcggctcgtt gagcataccg tcgccaaggt
     2221 ttttacccaa cgcgacaaac gacaggtcgc cagactgcgc atacaccagc gccataccaa
     2281 acagcaggaa agaagacgct gcggcagaaa ggatggtgta tttgatactg gcttccagtg
     2341 aacgtttctg gcggaaagcg taaccgacca ggccaaacag cggcaaagag atcagttcga
     2401 taccgaggaa cagagacgcc agatggttgg cattcgccag caggatcccg cccagcgcgg
     2461 caattaacac caacaggtag aactcgtcct tgttgtcgtt atagccttca agccacgggt
     2521 aggcgaaagt acaggtggcg aggctcgcca acaataccag cccggtgtaa agcatggcga
     2581 aaccatcaac gcgcatcagc ggcgtaacgt ccatagcgcc tgcctggccg acaaaccaga
     2641 gcgaaaccag cgccgcgtta agcccaataa ccgagagcgt agcgttgagg aaatgattgc
     2701 gtcgccacgc aatggagagc atcacaacca ccaccgtcaa gccgacgatc agcaacggta
     2761 gcagtgcgat caggttttgt ggagttattg tcatggcgat ttacggcctt gtagtagtaa
     2821 cggaattaac aaaccactgc tggatattgc caatcgcgga gtgcgaggta tccagaatcg
     2881 gctgcggata gaagccctgc agtaccagca gcaccaccag caacaggatc ataaacagct
     2941 cacgcagcga catccctggc agttcctggc tggcaatctc gcttttcgct ttaccgaagt
     3001 aagcgcgatg caacatcgcc agcgaataaa cagatgcaaa gaccagccca aaggtagaga
     3061 taacggtaat caccgggaca acctggaagc tgccgaacag aatcataaat tcgccgacga
     3121 agttaccggt gccaggcatc ccaagcgttg ccaccgcaaa gaacagcgac agtgctggca
     3181 gccatttcat cttgctccac agaccgccca tcatgcgcat gtcgcgggta tggatacgtt
     3241 cataaagctg accacaaaga ataaacagac ccgccgccga caagccgtgc gcaatcatct
     3301 ggattaccgc gccctggtag gccaactggc tgccggtgta gatagcaatc agcacgaagc
     3361 ccatgtggga aaccgaggtg taggcgatca gacgtttgat atcggtctgg gcgaaggcca
     3421 tccacgcacc gtagaagatg ccgataacac ccagccacat agcaattggc gcgaactctg
     3481 ccgacgcgtt cgggaacagc ggcagggaga aacgcagcaa accgtaagcg gcagttttca
     3541 gcaagatccc cgcgaggtca acggaaccgg cggtcggagc ctgggagtgc gcatccggca
     3601 gccagccatg cagcggaacc accggcattt tgactgcgaa ggcgatgaag aagcccagca
     3661 ttaacaggta ttccgcacca ctggacattg gcgtattcag cagctcttca tagttgaagg
     3721 tccagacgcc ggtcgcattg tagtgaacaa aaaccagcgc caggatggca atcaacatca
     3781 ccagaccact cgcctgggtg taaatgaaga acttggttgc cgccgtgata cgcgttttac
     3841 cgtcagaggc tttatgcccc cacagtgcga tcaggaagta catcggcacc agcatcattt
     3901 cccagaagaa gaagaacagg aacatgtcga tggcaaggaa cacgccgata acgccgccca
     3961 ggatccacat caggttgagg tggaagaagc cctgatattt ttcgatctct ttccacgaac
     4021 atagtaccgc cagcacaccg agcagaccgg tcagcacgac catcagcagc gacagcccgt
     4081 caatggcgag atgaatagag atacccaaac gcgggatcca cggcatgtcg aattcagact
     4141 gccactgcgg aattccggcg gattgcgtca gtgaataacc gccctgcaac cacagttgca
     4201 gcgacagcgc cagcgtcaat cccatggtga tcagcgcgat ccagcgtggc accttgacgc
     4261 caaagcgttc ggtctgccag cacaggaagc cgccaataaa gggaattaat attagccagg
     4321 gtagtaacat ggcgatcttt attccttgta aaagtcccgt caggaccgga ttttcaacga
     4381 attctcacga cagaaaattc tcttcggact gggggtagtg tcggatgcac cgcgaaggcg
     4441 gcttatccga cctacgttct tatatgccca ggggcaatcc cacaatcctt aactcaacgc
     4501 agtaccatca acagtgccag caccacgacc gcaccgatgc tcatggatgc cacataccag
     4561 cgcagatagc cgttctcact taacagcaga cctttacctg caaagcggga aaggacagcc
     4621 ggaatgttca tcattgagtt cagcggatcg cgtttcagca accaggcaat acccaggaac
     4681 ggcctgacga acactttgtc gtacagccag tcaaagcccc aggcgttgta ccaccaggta
     4741 ctcagcaggc ggcccggcgc actgttggcg atggaggtca ccagagtacg tttacccagc
     4801 cacagccaag ctgccagcag aatgccgaca atcgcgacca cgccagaggt aatttccagg
     4861 gtcaacatgc tgccgtgcgc cagttccgtc gtttgcggaa gcacgccctg cagcggcggt
     4921 acaatcagtg cgccaacgaa ggtggaaagg atcagcagca caatcagcgg caggctgtga
     4981 gttacccctt tcacggcgtg agcgtgaatt tgttcttttc cgtggaagac gatgaaaatc
     5041 atacggaagg tgtagagcga ggtcataaac gcaccgacca gacctgccac catcagattg
     5101 atatgaccat tcgccatcgc acccgcgagg atctcatcct tactgaagaa gcccgcagtg
     5161 accagcggca gtgccgacag tgctgcgccg cccaccagga agcagagata aaccagcgga
     5221 atagatttac gcagaccgcc catcttgaag atgttctgtt cgtgatggca ggccagaatg
     5281 acggaaccgg atgccaggaa cagcagcgct ttaaagaacg cgtgagtcat caagtggaaa
     5341 atcgccgcat cccatgcctg cacgccaagc gcgaggaaca tgtagccaat ctggctcatg
     5401 gtagagtaag cgagaacacg tttgatgtcg gtctgtacca gcgcggcaaa accggccagc
     5461 agcagcgtaa ccgccccgac aatgcccacc agatgcagaa tttccggcgt catcaggaac
     5521 aggccgtggg tacgggcgat caggtagaca cccgcggtta ccatggttgc ggcgtggatc
     5581 agcgcggaga caggcgtcgg gcccgccatc gcgtcggcaa gccatgtctg caacggcaac
     5641 tgcgcagatt taccgaccgc accgcccagc agcatcagcg tcgcccacat cagcatgtta
     5701 ttgccgtcag caaagtgcgc tggtgccagt tccaccattt cgcggaagtt cagggtgccc
     5761 agttcgttgt aaagaatgaa cagtgcgaaa gcgaggaaaa cgtcacccac acgggtcacg
     5821 acgaacgctt tcattgccgc tgcgccattc ttcggatcgg tgtaatagaa accgatcagc
     5881 agataggagc acaggcccac gccttcccag ccgaggtaca tcagcagcag gttgtcggca
     5941 agcaccagaa ctaccatgct ggcgatgaac aggttggtgt aggcgaagaa gcgagagtag
     6001 ccctcttcac cgcgcatgta ccaggaggcg tacatgtgaa taaggaaacc cacaccagtg
     6061 accaccgaga gcatggtcag cgacaggccg tccagcacca ggttaaaacc gatgttaaag
     6121 tcgcctaccg acatccacgt ccacagcggc tggctgtatg cctgctcgcc gttagcgaag
     6181 aaatcaacgc cgataaaggc ggttaccagc gccgccaggc ccacagagcc aacgccgacg
     6241 atcgccgaaa cgttttcaga ccagcgccca cgggagaacg ccagcaggac gaagccaatc
     6301 aatggcaaaa taatggttaa ggcaagcatg ttcatccgcg catctcactt actgaatcga
     6361 tgttcaggtt ctggcgacga cggtgaagtt gcagcagcag cgcaaggccg atactcgctt
     6421 ctgccgccgc gaggctgatg gcgagaatgt acatcacctg accgtcggtc tgaccccagt
     6481 agcttccggc gacaacaaag gccagcgcgg aggcgttaat catgatttcc agaccaatca
     6541 gcataaacag cagattgcga cggataacca gaccggttaa gccaagaatg aataagattg
     6601 ccgcgaggat cagtccatgt tgtaagggga tcatgcgtgc tcctccgttt ttcttttcgc
     6661 gctgtcgtct ttacgattgc tcagcacttc acccgcacgc tcttcacgac cgacgtggaa
     6721 ggccacaacc agacctgcga gcagcagcat agaagccagt tccaccgcca gtacgtaagg
     6781 cccgaacagc gtaataccca ctgctttagc actgattggc gtaccgtcga taccctgatc
     6841 gttaacaccg aggatggcgt aaacaatcac caccagcatg atggccgaca aaattgccgg
     6901 accaatccac acctgcggtt tcagccactg gcgttcctgt tcgatttctg aaccgcccag
     6961 gttgagcatc atcaccacga acacgaacag caccataatg gcacccgcgt agacgataat
     7021 ttccagcgca ccggcgaagt aagcgcccag tgagaagaac accccggaaa tcgccagcag
     7081 cgaaataatc aggtacagca gtgcgtgtac cggattggta tgggtgatca ctcgcaaggt
     7141 cgcaagtatg gctatcaggc cacagatata aaaagcgaac tccattgccc ctctccttac
     7201 ggtaacaggc tcttgacgtc gataggcttg gcttcgttct ctgcttcgcc cttatctttg
     7261 ccgtcgattg ccatacctgc catccggtag aagttatatt ccgggtattt gcccggaccg
     7321 gagatcagca gatcctcttt ttcgtaaacc agatcctggc gcttgtattc ccccatttcg
     7381 aaatccgggg ttaactgaat cgccgtggtc ggacaggctt cttcgcacag accacagaaa
     7441 atgcagcgtg agaagttgat gcggaaaaat tccggatacc agcgaccgtc tttggtttct
     7501 gctttttgca gcgagataca gccgaccggg caggctaccg cgcagaggtt acaggctacg
     7561 caacgctctt cgccgtccgg gtcgcgggtc agaacgatac gaccacgata acggggcggc
     7621 agatagatcg gctcttccgg gtacattcgc gtttcgcgtt tggcgaacgc gtgcaggccg
     7681 atcatccaga tactacgaac ctgggtgccg aaacctacta acaattcttt taaggtcatg
     7741 gtcttattgc cccttattgc gcctgccaga gaatgacagc cgccgttacc agcaagttga
     7801 tcagcgtcag cggcaggcag attttccagc cgaaggacat tacctggtca taacgcggac
     7861 gcggtaacga cgcacgaatc aaaatgaaca tcatcataaa gaacgcggtt ttcagcgcga
     7921 accagatgaa tggcggtaac aacgggcctt gccagccacc gaagaacagc gtcaccatca
     7981 atgcagagat ggtcacaatc ccgatgtact cacccacgaa gaacagaccg aacttcatac
     8041 cggaatattc aatgtggtaa ccatccgcca gttcctgctc ggcttccggc tggtcaaacg
     8101 ggtgacggtg acataccgcc acgcccgcga tggcaaaggt aataaaacca aagaattgcg
     8161 ggataacgtt ccacacatgc gcctggctgt tgacgatgtc ggtcatgttg aatgaaccgg
     8221 cctgcgccac cacgcccatc agggaaagcc cgaggaacac ttcgtagctc agggtctgcg
     8281 cagaagcacg catcgcaccc agcaacgagt atttgttgtt acttgaccag cccgcaaaca
     8341 gcaccgcgta aaccgccaga cctgccatca tcaggaagaa caaaatcccg atgttcaggt
     8401 cggcaaccac ccaacccgga ctgactggca caatcgcaaa ggccagcagc agcgaggtaa
     8461 aggcaatcat cggtgccagg gtaaagatga cgcgatccga gaatttcggg atccagtctt
     8521 ctttaaagaa cattttgatc atgtccgcaa ccagctggag cgaaccgccc cagccaacac
     8581 ggttaggtcc gtaacggttc tggaacagac ccagcaggcg acgttcgcca aagctcatga
     8641 atgccccgca ggtgacaacc accagcagga tcaccaccgc tttgaggatg gtcagcagga
     8701 tctcaatcag ttccggtgat atccaactca ttgttgtgcc tccttgagat cctcaagatg
     8761 cgcgccagcc agcaccggag caataccgga catacccatc ggcaagccca cctgccctgc
     8821 cgtcagccct tcagcgattt caaccggcag cgtgaccgta ttgccatcgt aactaaagga
     8881 gacgtgtgta cctgcgttca cacctaactt cgcggcatcc gctgggttga gtttgatgta
     8941 cggctgcggc atacggctct ggaagaccgg agcacgctgt gacaattcat cgctgccaaa
     9001 caggtggtaa tacggcgcga tacgccattt cccgtcctgc ggctggaagc gtgccggtac
     9061 gctggtgaag taatccagac cattttcgct ggtttcaaac agacgcacgc ccggatcgcc
     9121 aaagcgcagt ttgccgccca cttcgtcctg gaatttgttc cacgcctgcg gggagttcca
     9181 gcccggcgcc caggcaaacg gcacttgcga acggtgcgca gtcggctggt tgttaccttc
     9241 catcgagaag gtgaacatgg tgtcaatatc ctgcggctga cgcggctcat gaacgctgat
     9301 attggcgcgc atggcggtac gaccgctgta acggtgcggt tcacgggcca gtttctgccc
     9361 acgaatacgg aatgtcgcat ccggcgcagc atctttgata cctgccagtt ccgggatttt
     9421 cgccacaaca gcgtcaatca catggtcgag ctgcgtccag tccacttcac ggctcagcag
     9481 ggtgctgtgc agcgagtgta accagcgcca gctttccagc atgacagttt tgctgtcgta
     9541 ataagcagga tcgtaaacct ggaagaaacg ttgggcgcgg ccttcgttgt tgatcaccgt
     9601 accgtcgctt tcagcaaaac tggcagcaga aagtaccaga tgggcgtttt ccataatcgc
     9661 tgtgcgttga tgatcaacca ccatcaccag cggcgcttta gccagcgcag cattcacgcg
     9721 ggtagcggaa gcgtgacgat gcagatcgtt ttccagcacc accaccgcgt cggcgcgtcc
     9781 ggtttccagt tcggtcaacg cttcttcaag cgaaccgcca cccataatgc ccagccccat
     9841 gctgttgacg gaacgggcaa tcatggtgat accaacgtca gcgccgcgac ctttcagggc
     9901 tttcgcgacg ttagccgcag cctgaatcac ctctatgcta ccggcgttcg tcccggagat
     9961 aatcaacggt ttcttcgcac ctgccagtgc ctgcacgatg acgtcgattt tgctttgcag
    10021 ctcaggttcg ataccgtcaa ccgctggcgc agagttatcc agcgcatggg cgatggcaaa
    10081 acctaaacgc gcctgatctt caaccggtgc gcggtaagtc cacgccgcga tatcatccag
    10141 acgggtgtca tcaacgttgg taacaaacag cggatgcttc gcacgttgac cgatgttgag
    10201 gattgccgca atctgccagt cagccacttt ctgtgctgcc gccatttcgc gcgctttacc
    10261 tttcacagcc tgacgcactg ccagcgcgac gcgcgcgccg gtctgggtaa cgtcttcgcc
    10321 cagcaccagt accgcatcgt aagattcgat ttcgcgcaga gccggagtat aaatgccgcc
    10381 ttcacgcagc actttcagcg ccagttgcag acgttcctgc tcaccgtgag cgataccggt
    10441 gtagaagttt tcttcgccca ccagttcacg cagcgcaaag ttgctttcca cgctggcacg
    10501 cggagaacca ataccgatca ctttcttcga ctgacgcaga atatctgccg cgccctgcat
    10561 tgcctgttcg gcgttgaggg taatgaaatc atcgccacga cgctgtactg gctgacgcgg
    10621 acgatccttc aggttgacgt aaccgtaacc gaaacgacca cggtcgcaga ggaagtagtg
    10681 gtttaccgta ccgttgtaac ggttttcgat acgacgcagt tcgccgtaac gttcaccggg
    10741 gctgatgtta cagccgatgg aacattgctg gcagatgctc ggcgcaaact gcatatccca
    10801 tttacggttg taacgctcgg agtgcgtttt gtcggtaaat acgccggtcg ggcaaatttc
    10861 gaccaggtta ccggaaaatt cgctttccag cgtgccgtct tccgggcgac cgaagtagac
    10921 gttgtcgtgc gcaccgtaaa cgcccagatc tgtaccgtca gcgtaatctt tgtagtaacg
    10981 cacacagcgg tagcaggcga tgcagcggtt catttcgtga gagatgaatg gccccaaatc
    11041 ctgattacgg tgggtacgtt tggtgaaacg gtagcgacgg aagctgtgtc cggtcatcac
    11101 agtcatatcc tgaagatggc agttaccgcc ttcttcacat accggacagt cgtgcgggtg
    11161 gttggtcatt aaccactcga ccacactttc acggaactgt ttcgcttctt cgtcgtcaat
    11221 ggaaataaag gtgccatcgg aagccggtgt catacaggac atcaccaggc gaccacgcgt
    11281 gtcttccgcg ttttggtatt gcttcaccgc acactggcgg caagcaccga cgcttcccag
    11341 cgccggatgc cagcaaaagt aaggaatatc aaggcccaga gacagacaag cttccagcag
    11401 gttgtccgct ccgttgacct cgtattcttt gccgtctaca tgaattgtag ccattagcat
    11461 gcttccagtt ttctcggtca gggaccgagc gttaatcgaa attcggttac cagcgctctt
    11521 tcagcaggtt cggctgaatc ccattaatca aatgggtatt gctgaacggc tgtttgattc
    11581 ccgcctcaaa ttcttcgcgg aaatatttga tggcgctctg taacggctcc actgcaccag
    11641 gtgcgtgggc acagaaagtt ttacccgggc ctaagaatcg acacagttgc tcaagtgttt
    11701 cgatatcgcc cggctggcct tcgccacgct ccagcgcacg cagaattttc acgctccacg
    11761 gcagaccgtc gcggcacggc gtacaccagc cgcaggactc acgggcgaaa aactcttcca
    11821 ggttacgcac cagcgacacc atgttgatct catggtcaac cgccatcgcc agcgccgtac
    11881 ccagacggct gcccgcttta ccgatacttt cgaattccat cggcagatca aggtgcgctt
    11941 cggtcaggaa gtcagtcccc gcgccgcctg gctgccaggc tttaaatttc agaccatcac
    12001 gcataccacc ggcgtaatct tcgaggatct cgcgtgcggt ggtgccgaac ggcagttccc
    12061 acagtcccgg atttttcacc cgaccggaga agcccatcag cttggtgcca gcatctttac
    12121 ttttcgagat gttctgatac cactccacgc cgttagcgag gatcgccgga acgttacaca
    12181 gggtttcgac gttgttgaca caggtcggtt tgccccatac gccggaggtt gccgggaagg
    12241 ggggtttcga gcgtgggtta gcacgacgcc cttccaggga gttgattaac gctgtttctt
    12301 ccccgcagat gtagcgccct gccccggtat ggacgaacag ttcgaagtcg aaacctgttc
    12361 ccataatgtt tttgccaagc aagcccgctt cagtggcttc ggcaatggcg cggcgcagat
    12421 ttactgccgc ttcgatatat tcgccacgca ggaagatgta gccacggtaa gctttcagcg
    12481 caaacgcgga gatgagcata ccttccacca gcaggtgcgg cagttgctcc atcaacaggc
    12541 ggtctttata ggtgcccggc tccatttcat cggcgttaca cagcaggtaa cggatgttca
    12601 tggattcgtc tttcggcatc aggctccact tcaggccagt cgaaaagccc gctccgccgc
    12661 gccctttcag accagcgtct tttacctgat taacgatttc gtccggagac agcccggtca
    12721 gcgccttacg cgccccttcg taaccgtttt tgctgcggta ttcgtccagc cacactggct
    12781 gtttgtcatc gcgcagacgc caggtcagcg gatgcgtttc gggagtacgg ataatgtttt
    12841 tcatttatac cgctccagca gttcagggat cgcttccggg gtcagatgcg cgtgagtgtc
    12901 ctcatcgatc atcatgtttg gccctttatc acagttcccc aggcagcaag ttggcagcag
    12961 cgtaaagcgg ccatcaaatg tcgtttgccc tggtttgatg ttcagctttt tctcgagcgc
    13021 cgcctgaata ccctgatacc cgttgatatg acagaccacg ctgtcgcaat aacggatcac
    13081 atggcgacca accggctggc ggaagatctg actgtagaac gttgccacac cttcgacgtc
    13141 gcttgccgga atacccagca catcggcgat cgcgtggatc gcaccatccg gcacccagcc
    13201 acgctgcttc tgaacgattt tcagcgcttc aatggacgcc gcacgcgggt cttcgtagtg
    13261 gtgcatctcg tgctcaatcg cttcacgctc tgccgcactc agctcaaaag cctcggtttg
    13321 tggttgttga ttctcgtgca taattagcgg tccacatctg acataacaaa atcgatactg
    13381 cccagataaa caatcaggtc agacaccagg ctgccgcgga tcgccgccgg aatttgctgc
    13441 aaatgcgcat aactcggcgt gcggatacga gtgcgataac tcatggtgct accgtcgctg
    13501 gtcaggtagt aactgttgat ccccttggtc gcctcaatca tctggaaaga ttcattggca
    13561 ggcatcaccg gcccccacga cacttgcagg aagtgggtga tcagggtttc gatatgttgc
    13621 agcgtgcgct ctttcggcgg cggcgtggtc agcgggtgat ccgctttgaa cgggccttcc
    13681 ggcatgttgt tgaggcactg ctcaagaatg cgcagactct ggcgcagctc ttccactttc
    13741 agcattacgc gggtgtagca gtcagaaaca ccaccaccaa ccgggatttc aaagtcgaag
    13801 ttttcatagc cagaataagg acgcgcctta cgcacgtcga agtcgatccc ggtagcacgc
    13861 aggcccgcgc cagtggtgcc ccactccagc gcctctttcg cgccataggc ggcaacgccc
    13921 tgggaacgac ctttcagaat ggtattttgc agcgccgctt tctcgtaaga cgccagacgt
    13981 ttcggcatcc agtcgaggaa ctcacgcagc aggcgatccc agccgcgcgg caggtcgtgc
    14041 gctacgccgc caatacggaa ccacgccggg tgcatacgga aacccgtgat cgcttccacc
    14101 agatcgtaaa ttttctgacg atcggtaaag gcgaagaaca cgggcgtcat tgcgccgacg
    14161 tcctgaataa aggtcgagat gtacagcagg tggctgttga tacggaacag ttcggagagc
    14221 ataacgcgaa tgacgttaac gcgatccggc acggtgatcc cggccagttt ctctaccgcc
    14281 agcacgtaag gcatttcgtt aacgcagccg ccgaggtatt caatacggtc ggtatacgga
    14341 atgtagctgt gccaggactg gcgctcgccc attttctccg caccacggtg gtggtagccg
    14401 atgtctggta cgcagtcgac aatctcttcg ccgtcgagtt gtaaaacgat acggaaagcc
    14461 ccgtgcgccg acgggtggtt cggaccgagg ttgaggaaca tgaagtcctc gttttcggtg
    14521 ccgcgcttca tcccccactc ttccggtttg aaggtcaggg cttccatctc cagatcctgt
    14581 ttggctttgg tcagctcaaa cggcgagaat tcggtagcgc gcgccgggta atctttacgc
    14641 agcgggtgac ctttccaggt ttgcggcatc atgatgcggc gcaggttcgg gtgaccgtcg
    14701 aaagtaatgc caaacagatc ccaggtttca cgctcatacc agttagcgtt cgggaacagt
    14761 ttggtgaagg tcggtacgtg caggtcgttt tctgccagcg ccaccttcag catgatgtcg
    14821 cggttacgat cgatagaaat cagatggtag aaaacggaaa aatccgcggc aggtaaccct
    14881 tcgcggtgtg tgcgcagacg ttcgtccatg ccgtgtaagt caaacagcat gacgtaaggt
    14941 ttcggcagtt tctttaagaa atcgccaact tccagtaatt gttcacgctt gatccacaca
    15001 acgggaaccc cggtgcgagt cgcctgaaca gtaaaggcat ccggcccaaa acggttgcgc
    15061 agttcgccaa tcaccggatc atcaagatga tcacgggtct gccaggcggg ttcttgcgcg
    15121 gttaagtcgg tcatattgtt caccattgca aatggtccgt ggtgactgtc ggcgcagtaa
    15181 gcttcgcgaa atagatttag gaataagcga agaaaaatcc ctttgccgac aggcgcaaat
    15241 taaatctcgt caggtgtacg caggttggtt acggcaatgc gttcaccgcg cttgcgttcg
    15301 cgctctgatt gcatattggc gcgataaacg ccctgatcgc caaccaccca ggagagcgga
    15361 cgacgttctt tgccgataga ttcctgcaac agcatcagtg cctgcatgta cgcttcagga
    15421 cgcggcgggc agcccgggat atacacatca accgggatga atttatcgac gccctgcaca
    15481 acggaataaa tatcgtacat accaccagag ttggcacagg cacccattga gataacccat
    15541 tttggttcca gcatctggtc atacagacgc tgaataaccg gtgccatttt ggtaaagcag
    15601 gttcctgcaa ccaccatcag gtcagcctga cgcggcgaag cacgcaatac ttctgcgcca
    15661 aaacgcgcca cgtcatgcac cgcggtaaac gaagtcacca tctcaacgta acagcaggaa
    15721 agaccgaagt tatacggcca aattgagttt ttacgacccc agttaaccat gtcattgagc
    15781 ttgcccataa acacgttttt gttaacttct tgctccagag ggtcggttac gatctcctgc
    15841 ttttgcaggg ggtaacggtc gttctcaccg ttgggatcta tgcgggtgag cgtataatcc
    15901 atcttaatgc ctcgcggtta gcgttgacga ttagcgatac tgttcgtttc cgggttcata
    15961 cgctcgcggc gtgaacgcgc gggcgtccag tccagcgcgc caatacgcac cagataaacc
    16021 agacctgcca gtaacacaaa aataaaaatt gcagcttcca caaagcctac ccagccgctt
    16081 tcgcggatag aggttgacca tgcgaacaga tacagcgctt caacgtcgaa gataacgaag
    16141 aacatggcca ccagataaaa cttggcggac aggcgtaagc gggcggagcc gaccgagtcg
    16201 ataccggatt caaacggcac gtttttcgac ctcgcgcgtg cgcgaccgcc taaaaaccaa
    16261 ccgcctacca gcatcaggca acacaggcca atggcaacga taagaaagat agcgaatgcc
    16321 cagtgatgag cgatgacttc agtggatgtt gacatactca ttgcttactc atcaaaagta
    16381 gcgccagatt cactgctctt cacggcagat ggacgccaca tcgattcatg gggaggaata
    16441 aaaaaaacct tacaatcact gtagaaattc ttttatacag ctaattgatg tggtctttta
    16501 ctcctttcta taaccttttg tcaactttaa caaaagtttc ttcacattag tttacataat
    16561 atcaacacca ttagcattta atgccctttc acgccagatc cttgacgact ccaggataat
    16621 tagatgttgt tgaatcgtgt ccgttgtgaa gcaatggaaa aaatacgggt ctattttgac
    16681 aggaatttgt gtcgattcct ccccccaaaa gagagtattt tcttgatctg tgacacgctt
    16741 ttgtcattcc ataacaaaaa cgcagcaaca aatttacgta tttttaacat cattgtagca
    16801 ggtgattttt ttcaggcgat tatttgtgcg ttcgggacgt gaatctctgg tgattgaaaa
    16861 atgaacagtt ttgtacgttc tgcactatgc gaggaaggat ttttactaaa aaaaagccgc
    16921 tggggtttaa aacaccccca gcggctcgtt tttacactat tgtctcagga attatctatc
    16981 gtccgtcgat tactcgatat ccctttcaat caacaatgaa tcatcccctt ccggagcaga
    17041 cattgggctg tattgccacg gattatggta gctctccatc gcctgataga tcacctgggc
    17101 cagctcatta ttactggacg ggtcatagca cagcaaatat tcggtatcag gtagcggcgg
    17161 taagccatct actccactca gcacacgcag gtccgggctc atcatttcaa tcggccttgc
    17221 cgtcacgcca agaccggctt tcactgccgc acgaacggcc ggaagcgtcg aggcgacata
    17281 agccagtcgc catggaatat ctgctttatt aagcgtcgcc agcaccatat cgcgaaacgg
    17341 acttggatca tccagcaata caagagggat cggctcgcct ttttgcaata cgtattccgc
    17401 tgcgcagtac cagtgtgttg gcgaggtacg caggttcaat gccttaaacg ctgaaggacg
    17461 atgggtggtt accatcaaat ccacttcctg ggattccagc atttctgcca tataggcatt
    17521 acgtttaacg cggacatcca gcgctaattt cggataaacc gaactcacgc gatttaacag
    17581 gaaaggtaag atcgtatcgg cagattcatc tgaagcaccg atagttaaca cgccctgaag
    17641 attactgaac attaatgatg agcaggcctc atcattaaaa cgcagtattt tcctggcgta
    17701 accaagaagt tgaatgccat gttcagttaa cagtttgttg cgaccgtgac gagcgaacag
    17761 ttctttccca acgagttgtt ccagacgctg catttgctga cttacggcgg actgagtacg
    17821 acacacagcg gcagctgcgg cagcaaaagt gttcagatcg gcaacagcaa caaatgttct
    17881 cagcagatcg aggtcgaggt taattatcgg acgatttgca cttatcatat attatcactt
    17941 actggcggct catactgagc tggttaatgc tgtgcacaca caaacaagca attccatttg
    18001 taatgtgcct ccctggcagt ttcatccgga acccggacga aagtaaaaat gcatatgagt
    18061 tgcactaaaa aagcgactca cattgttccg ttataatgcc tgaagtagat cacagaatat
    18121 atcttcaggg atcgcatatc tattaagtta ctcactcttt tctatttatg acatgcgcgt
    18181 gtttgtataa atgtaaatgt gagtccttgt tccactctcg tgcagcatcg ctggtcatac
    18241 gcgaacacgt acaaacagca atggtgaggc ggcatcaaga gcagggatcc gtcatttatg
    18301 cgagcatttt accccaaaaa ccttttattt ataagggtca ttgcgaatta tctgatgcaa
    18361 agttatgtta tgttaggcaa agtaatcttc tattattaat aagcacatca aaaccttttt
    18421 gaatattaaa taataattaa ttagcatcat cctcattcat taattccgct taacaatagt
    18481 ttcacacctt gcccccattg cgacacctcg gaaatcatca cgctgttaaa ctctccgtaa
    18541 tgcggaaata tcatcatcgc gtttcattag tgaattctta aatgaggcat tttcacacaa
    18601 ttatcttaca gataaaaaaa ccagacttgc aattaagaat cagaacaatt cactatataa
    18661 cattgcatgc aaagcatata cacctcatta ttttgtcatt attaagttat taacagcaca
    18721 atcgagcctt cccctctggc aaaatcttat tctgcagacc ttcaaaacac cgtcctgggg
    18781 gagtacattg ttctaagctg acttccacgg cagggagtgg cgataacagc aaaaaaggtc
    18841 aagattcatg tcccccattg aaaaatccag caaattagag aatgtctgtt atgacatccg
    18901 tggtccggtg ctgaaagaag caaaacgcct ggaagaagaa ggtaacaagg tactgaaact
    18961 gaacatcggc aacccagccc cattcggttt tgacgcgcca gatgaaatcc tcgttgacgt
    19021 gatacgcaac ctgcctaccg ctcaagggta ttgcgattcc aaagggcttt actccgcgcg
    19081 taaagccatc atgcagcact accaggctcg tggcatgcgt gatgttaccg tggaagatat
    19141 ttacatcggc aatggtgtat cggagcttat cgttcaggca atgcaggcat tgctgaacag
    19201 cggggacgaa atgttggttc ctgcaccaga ttacccactc tggaccgcgg cggtttcgct
    19261 ttccagcggt aaagcggtgc attatctttg cgatgaatcc tctgactggt tcccggacct
    19321 cgatgatatt cgcgctaaaa ttacgcctcg tacgcgtggg atcgttatta tcaacccaaa
    19381 taacccaacc ggcgcggtat attccaaaga gcttttaatg gagattgtgg agattgcacg
    19441 tcagcataat ctcattatct tcgccgatga aatttatgac aaaattctct acgacgatgc
    19501 tgagcatcac tcaattgcgc cactggcacc tgacctgctg accattacct ttaacggact
    19561 gtcgaaaacg taccgcgttg caggcttccg tcaggggtgg atggtgttga acgggccgaa
    19621 aaaacacgcc aaaggctaca tcgaaggtct ggaaatgctg gcttcaatgc gcctgtgtgc
    19681 taacgttcct gcgcaacacg ccattcagac cgcgctgggt ggttatcaga gcatcagtga
    19741 atttattacc cctggcggtc gtctttatga gcagcgtaac cgcgcgtggg aactgatcaa
    19801 cgatattccg ggcgtttcct gcgtgaaacc tcgtggtgcg ctgtatatgt tcccgaaaat
    19861 cgacgccaaa cgctttaaca ttcacgacga tcagaaaatg gtgctggatt tcctgttgca
    19921 ggaaaaagtt ctgttggtgc aagggacggc attcaactgg ccgtggccgg atcacttccg
    19981 cattgtcacg ctaccgcgtg tcgatgatat cgagctgtct ttgagcaagt tcgcgcgttt
    20041 cctttctggt tatcatcagc tgtaatctta atttcactgc cggagattgc atccggcagc
    20101 gttatcccgc cacaatgacc tgatgatgtc atcatacgta aggtcactat gaaacagagc
    20161 catttttttg cccatctctc ccgcctgaaa ctcattaacc gctggccgct aatgcgcaac
    20221 gtgcggacgg aaaatgtgtc cgaacacagt ttgcaggtag cgatggtcgc ccatgcgctg
    20281 gcagctatca aaaatcgaaa atttggcggt aatgtcaacg ccgaacgtat cgctttactg
    20341 gcgatgtacc acgatgcctc agaagtgctc accggcgatc tccctactcc ggtgaaatac
    20401 ttcaattcgc aaatcgctca ggagtacaag gctattgaaa aaatcgctca gcaaaaactg
    20461 gtcgatatgg ttccggaaga gctgcgggat atctttgcgc cgttaattga cgagcatgca
    20521 tatagcgatg aagaaaaatc gctggtgaaa caggcagatg cactgtgtgc atatctgaaa
    20581 tgtctggaag aactcgcggc cggaaataat gaattcttgc tggcaaaaac gcgactggaa
    20641 gcgacgcttg aagcgcgtcg cagccaggag atggactact tcatggaagt atttgttccc
    20701 agcttccatc tttcgctcga tgagattagc caggattcac cgctgtaagc agccggagtc
    20761 tgcgtcgcat caggcaataa gcgccggatg cgacatcagg ctcttgtcaa aacggaaaca
    20821 gcatcgggat catcaccaca caaaccgcca tcacgataat ggtgaacggt acccccaact
    20881 tcacaaagtc actgaagctg taatttcccg gacctaaaac cagtgtgtta acaggtgaag
    20941 aaaccggtgt cataaaggcg gcggatgctg ccatcgccac gaccatcgcg aatggataag
    21001 gcgacacccc catcgttttg gcagcagcca gcgcaatcgg agccatcaac accgccgtcg
    21061 cggtattaga gataaatagc ccaataaccg ccgacaagac aaacaaacac cccagcatca
    21121 tatgtggccc gtaaccaccg ccaatgtcca tcagcccttt caccgccagc gcgacacctc
    21181 ccgttttctg taatgccaca gcaaacggca tcatcccaac gatcaaaata atgctcggcc
    21241 agtgaatgga tttataggcg ctttcagcat ctatacagcg gaatttcccc atcagcaggc
    21301 aggcgatgat agcggcaaca ggattaggaa tttcatctgt cagcattaac gccaccatta
    21361 gcaccagaca gaaaatggca tggggtgcct ggctgtgcgc gggcgatgct tcactcacct
    21421 cttccggtaa gttcagcgct acgaagtcgc ggccctgttt ggccagcata ccgatcagtt
    21481 tccagttacc cacaaccagg atgatatcgc ccagcagcag aggctcatcc gccagcgaac
    21541 cttccagcgc cacgccattg cgctttagcc ccaccacatt cagtccgtag cgggtacgaa
    21601 aaccaatttc gcgcaccgat ttaccaatca gttctgactc aggaattaat gaaatctctg
    21661 ccatgcccac atcaagggcc tggtcagaaa aatactcgcc gcgcagtacc atcggctcca
    21721 gcaattgctc actacaaaat tgccggagat cgacatcagc cgcagacata tcaataagca
    21781 aaacgtcacg cgcgcgaaat tcagaaaccc cattaacgtt cacgataacg cgacgaaaac
    21841 gccgccagcg ttcaacaccg atgacgttag cgccataacg ctcacgtaat ttgagatcat
    21901 ccagccgttg accaatcatt ggcgatccgg ggcgaatagc cagacgtcgc gcacgcccgg
    21961 tcagtcgata ttcacggata agatcgcgaa aggttcgacg cgtccagcct tcgcgctgcg
    22021 gggtctgggt atcccctttc agcatgaaac gcatcactaa catatacaaa atacccagca
    22081 ccagcacgac caggccaata ggtgttacgc taaagaaact gaagccgtga tagccttcac
    22141 gcagcaattc actgttgact accaggttcg gcggcgtcgc caccagcgtc atcatgccgc
    22201 ttatcagccc ggcaaaactc aacggcatca tcagacgtga cggcgaagtt tgcatacgca
    22261 tggcaacgct taacaccacg ggaataaaga tagcgacaac gccggttgaa ctcataaacg
    22321 cgccaagccc cgcgacggtc agcatcaaca aaaccaacat tttgatttca ctgttgcccg
    22381 caactttgac cagccatgtt cccattacgg tggcaacacc ggtacggacc aaaccatcgc
    22441 caataataaa caaggcggca atcaggacaa cgttaggatc agaaaagccg gaaaatactt
    22501 cggggactgt cagcgttccg cttaatgcaa acgcgacaat aacaaacaaa gcgaccgcat
    22561 ccatacgcac tctgcccgtc gcaaacaaga caatggcaac ggccagtaat gaaagaaccc
    22621 aaatcaattc accgttcaca acttatcctt gttaattgag ggagatgtct tgattctgcc
    22681 ataaaaaagc cccgacgaga cggggctaaa tcatgatcag gtgtttcact gaataataac
    22741 atcgccattt ggctgtttgg tcacagtaat ttgctccaga ctgtggagga ccaaatcgac
    22801 ctcattcagg cgcggggtat ctgccggagc gttaaccgca atgacatgac aacccgccgc
    22861 caggccagaa agcacgccag cggagcatct tccaccacca cacactcctg cggcgcaagc
    22921 cccagcagct gcgcgcctaa cagatacgca tcaggttctg gttttccgcg cttcactcgc
    22981 tcagcggtta caaacacctc tggtgcggga agcccagcta ttttatggcg cgctcgcgct
    23041 accggcatgg agccagaagt cacaatggcc cacggaatac ctgctttatt caaatgactg
    23101 agtaaggcga ttgcccccgg aagcgggtaa taccttcggt ttccgtggcc tcgatgtgct
    23161 ccagacgcgt aaactcggcg gcaatatcag cctcggattt gcccgccata aaatggcgca
    23221 gagaggtgat cgcctgttta ccgtgaatga aagccagcac ctcttccggc gctaacccat
    23281 gacgtctggc ccagttgctc cacgcccgtt ctaccgcagg cagggaatcc accagcgttc
    23341 catcaagatc aaacagaaaa cctttgcacc gcacgcgggc ctcctcaggc attgataatt
    23401 tgattaattt cgttggcgct caaatggtac tgacgcgggc aggcatgcca cacattaagc
    23461 atgcgctgat atttttccca cattggcgtc tgggcgttaa aaccgtgagt tccggcgtca
    23521 aaatgggtat agcgcccttc cacattaacc ataaagcgga cataaccgag gtaacgtgct
    23581 tcagtggcgg cgtcaaagcc gaggaaggtg acacgacgtt catcgatgga ttgctggtcc
    23641 tgcaaattag accaggaaac atgcaacgca tgatacatct ccataatgtc gatgatagtg
    23701 cggcaggttt cttctttcag ctcgccaaac tcgcgatcca attcacgcat ctgtaatccg
    23761 taacctcgct caataattgt ttgcaggcga cggtaacgtt cagcatttgc cggatcgagc
    23821 atagtcatca tcttgtactg attagacaaa ataagacgtt gcgcgttggt catttccatt
    23881 gttgactcct gtatcactct actacggtga aaaaaaagaa ggctgagtat gccttctttt
    23941 atatgcgtaa tcaggggtca attacaaatc atcaaggaaa gttttatcca gttgtttgaa
    24001 ggcgcgctta agcgtgtcag ctaatgcctg gtaatcaggc ttgccttcaa cgggtgccaa
    24061 cacctgtcca gactcctgca atttaccgcg aacttcataa aaccagttaa ggattgcagg
    24121 gggtaatggc gttacagaac gcttgcccag ccaccacaat ccctgcatgg gtaaacttaa
    24181 ggcgaacagt gcagtggcaa ctgccggccc aagctgaccg cccagggcaa tctgccagca
    24241 gagagtaaat acggcgatcg gcggcataaa acggatcgca taacgcgtca tcttgataac
    24301 gcgattttcg acaaagaccg gggcaaggcg tttttccagt ggccacgtct ttgagtaatg
    24361 ctgtccgcgg cgaaacaagc taaaaaaatt aacagaacga ttatccggcg ttgacatgct
    24421 tcacctcaac ttcacatata aagattcaaa aatttgtgca aattcacaac tcagcgggac
    24481 aacgttcaaa acattttgtc ttccataccc actatcaggt atcctttagc agcctgaagg
    24541 cctaagtagt acatattcat tgagtcgtca aattcatata cattatgcca ttggctgaaa
    24601 attacgcaaa atggcataga ctcaagatat ttcttccatc atgcaaaaaa aatttgcagt
    24661 gcatgatgtt aatcataaat gtcggtgtca tcatgcgcta cgctctatgg ctccctgacg
    24721 tttttttagc cacgtatcaa ttataggtac ttccatgtcg agtaagttag tactggttct
    24781 gaactgcggt agttcttcac tgaaatttgc catcatcgat gcagtaaatg gtgaagagta
    24841 cctttctggt ttagccgaat gtttccacct gcctgaagca cgtatcaaat ggaaaatgga
    24901 cggcaataaa caggaagcgg ctttaggtgc aggcgccgct cacagcgaag cgctcaactt
    24961 tatcgttaat actattctgg cacaaaaacc agaactgtct gcgcagctga ctgctatcgg
    25021 tcaccgtatc gtacacggcg gcgaaaagta taccagctcc gtagtgatcg atgagtctgt
    25081 tattcagggt atcaaagatg cagcttcttt tgcaccgctg cacaacccgg ctcacctgat
    25141 cggtatcgaa gaagctctga aatctttccc acagctgaaa gacaaaaacg ttgctgtatt
    25201 tgacaccgcg ttccaccaga ctatgccgga agagtcttac ctctacgccc tgccttacaa
    25261 cctgtacaaa gagcacggca tccgtcgtta cggcgcgcac ggcaccagcc acttctatgt
    25321 aacccaggaa gcggcaaaaa tgctgaacaa accggtagaa gaactgaaca tcatcacctg
    25381 ccacctgggc aacggtggtt ccgtttctgc tatccgcaac ggtaaatgcg ttgacacctc
    25441 tatgggcctg accccgctgg aaggtctggt catgggtacc cgttctggtg atatcgatcc
    25501 ggcgatcatc ttccacctgc acgacaccct gggcatgagc gttgacgcaa tcaacaaact
    25561 gctgaccaaa gagtctggcc tgctgggtct gaccgaagtg accagcgact gccgctatgt
    25621 tgaagacaac tacgcgacga aagaagacgc gaagcgcgca atggacgttt actgccaccg
    25681 cctggcgaaa tacatcggtg cctacactgc gctgatggat ggtcgtctgg acgctgttgt
    25741 attcactggt ggtatcggtg aaaatgccgc gatggttcgt gaactgtctc tgggcaaact
    25801 gggcgtgctg ggctttgaag ttgatcatga acgcaacctg gctgcacgtt tcggcaaatc
    25861 tggtttcatc aacaaagaag gtacccgtcc tgcggtggtt atcccaacca acgaagaact
    25921 ggttatcgcg caagacgcga gccgcctgac tgcctgattt cacaccgcca gctcagctgg
    25981 cggtgctgtt ttgtaacccg ccaaatcggc ggtaacgaaa gaggataaac cgtgtcccgt
    26041 attattatgc tgatccctac cggaaccagc gtcggtctga ccagcgtcag ccttggcgtg
    26101 atccgtgcaa tggaacgcaa aggcgttcgt ctgagcgttt tcaaacctat cgctcagccg
    26161 cgtaccggtg gcgatgcgcc cgatcagact acgactatcg tgcgtgcgaa ctcttccacc
    26221 acgacggccg ctgaaccgct gaaaatgagc tacgttgaag gtctgctttc cagcaatcag
    26281 aaagatgtgc tgatggaaga gatcgtcgca aactaccacg ctaacaccaa agacgctgaa
    26341 gtcgttctgg ttgaaggtct ggtcccgaca cgtaagcacc agtttgccca gtctctgaac
    26401 tacgaaatcg ctaaaacgct gaatgcggaa atcgtcttcg ttatgtctca gggcactgac
    26461 accccggaac agctgaaaga gcgtatcgaa ctgacccgca acagcttcgg cggtgccaaa
    26521 aacaccaaca tcaccggcgt tatcgttaac aaactgaacg caccggttga tgaacagggt
    26581 cgtactcgcc cggatctgtc cgagattttc gacgactctt ccaaagctaa agtaaacaat
    26641 gttgatccgg cgaagctgca agaatccagc cctctgccgg ttctcggcgc tgtgccgtgg
    26701 agctttgacc tgatcgcgac tcgtgcgatc gatatggctc gccacctgaa tgcgaccatc
    26761 atcaacgaag gcgacatcaa tactcgccgc gttaaatccg tcactttctg cgcacgcagc
    26821 attccgcaca tgctggagca cttccgtgcc ggttctctgc tggtgacttc cgcagaccgt
    26881 cctgacgtgc tggtggccgc ttgcctggca gccatgaacg gcgtagaaat cggtgccctg
    26941 ctgctgactg gcggctacga aatggacgcg cgcatttcta aactgtgcga acgtgctttc
    27001 gctaccggcc tgccggtatt tatggtgaac accaacacct ggcagacctc tctgagcctg
    27061 cagagcttca acctggaagt tccggttgac gatcacgaac gtatcgagaa agttcaggaa
    27121 tacgttgcta actacatcaa cgctgactgg atcgaatctc tgactgccac ttctgagcgc
    27181 agccgtcgtc tgtctccgcc tgcgttccgt tatcagctga ctgaacttgc gcgcaaagcg
    27241 ggcaaacgta tcgtactgcc ggaaggtgac gaaccgcgta ccgttaaagc agccgctatc
    27301 tgtgctgaac gtggtatcgc aacttgcgta ctgctgggta atccggcaga gatcaaccgt
    27361 gttgcagcgt ctcagggtgt agaactgggt gcagggattg aaatcgttga tccagaagtg
    27421 gttcgcgaaa gctatgttgg tcgtctggtc gaactgcgta agaacaaagg catgaccgaa
    27481 accgttgccc gcgaacagct ggaagacaac gtggtgctcg gtacgctgat gctggaacag
    27541 gatgaagttg atggtctggt ttccggtgct gttcacacta ccgcaaacac catccgtccg
    27601 ccgctgcagc tgatcaaaac tgcaccgggc agctccctgg tatcttccgt gttcttcatg
    27661 ctgctgccgg aacaggttta cgtttacggt gactgtgcga tcaacccgga tccgaccgct
    27721 gaacagctgg cagaaatcgc gattcagtcc gctgattccg ctgcggcctt cggtatcgaa
    27781 ccgcgcgttg ctatgctctc ctactccacc ggtacttctg gtgcaggtag cgacgtagaa
    27841 aaagttcgcg aagcaactcg tctggcgcag gaaaaacgtc ctgacctgat gatcgacggt
    27901 ccgctgcagt acgacgctgc ggtaatggct gacgttgcga aatccaaagc gccgaactct
    27961 ccggttgcag gtcgcgctac cgtgtttatc ttcccggatc tgaacaccgg taacaccacc
    28021 tacaaagcgg tacagcgttc tgccgacctg atctccatcg ggccgatgct gcagggtatg
    28081 cgcaagccgg ttaacgacct gtcccgtggc gcactggttg acgatatcgt ctacaccatc
    28141 gcgctgactg cgattcagtc tgcacagcag cagtaacctc gtcatcatcc gcagctttgc
    28201 gctgcggata tctgaaccgg aaataatcac tatttccggt tttttattct cttaatctgc
    28261 attaatcctt tctgattatc ttgcttaact gcgctgcatc aatgaattgc gccatttcac
    28321 tttacatact taccactttg ttttgtgcaa gggaatattt gcgctatgtc cgcaatcact
    28381 gaatccaaac caacaagaag atgggcaatg cccgatacgt tggtgattat cttttttgtt
    28441 gctattttaa ccagccttgc cacctgggta gttccggtgg ggatgtttga cagtcaggaa
    28501 gtgcagtatc aggttgatgg tcaaacaaaa acacgcaaag tcgtagatcc acactcattt
    28561 cgcattctga ctaacgaagc aggcgaacct gagtatcacc gcgtacagct gttcacgacg
    28621 ggcgatgaac gcccgggcct gatgaacttc ccgtttgaag gattaacctc aggatcgaaa
    28681 tacgggacag ctgttggcat catcatgttt atgctggtga ttggcggcgc gtttggcatt
    28741 gtgatgcgta caggaaccat tgataacggt atcctggcgc ttattcgcca tacccgcggg
    28801 aatgaaattc tctttattcc tgcgctgttt attctgtttt cacttggcgg cgcggtattt
    28861 ggtatgggag aagaggccgt cgcctttgcc attatcatcg caccgctaat ggtccggctg
    28921 ggctatgaca gtattaccac cgtcctggtg acctatattg ccacgcaaat cggttttgcc
    28981 agttcgtgga tgaacccgtt ttgtgtggtc gttgctcagg ggattgccgg cgttccggtg
    29041 ctttctggct ccgggttgcg catcgtggtg tgggttatcg ccactatgat tggcctgatc
    29101 tttaccatgg tgtacgcctc acgagtgaaa aagaatcctc ttctgtcacg cgtgcatgag
    29161 tccgaccgct tctttcgtga aaagcaggcg gatgttgaac aacgtccgtt tacctttggt
    29221 gactggctgg tattgattgt cctgaccgcc gtaatggtct gggtgatttg gggcgtgatc
    29281 gttaatgcct ggtttattcc agaaattgcc agccagttct tcaccatggg tctggtgatt
    29341 ggcatcatcg gcgttgtttt ccgccttaac ggcatgacgg ttaataccat ggcttcatcc
    29401 tttaccgaag gggcgcgaat gatgatcgcc cctgccctgc tggtgggttt cgccaaaggg
    29461 attttgctgc tggtcggtaa tggtgaagcg ggtgatgcca gcgtgttaaa taccatcctc
    29521 aacagcattg ccaatgccat tagcggtctg gacaacgcgg tcgcggcctg gtttatgttg
    29581 ctcttccagg cggtatttaa tttcttcgtg acgtccggtt ctggtcaggc ggcgttaacc
    29641 atgccgttac tggcaccgct tggcgatctg gtcggtgtta accgtcaggt taccgtgctg
    29701 gcattccagt ttggtgatgg cttcagtcac atcatttacc caacctcagc ttcgttaatg
    29761 gcaactctcg gtgtgtgcag ggtggacttc cgtaactggc tgaaggtggg cgcgacccta
    29821 cttggactgc tgtttattat gtccagcgtc gtagtgatcg gcgctcagtt gatgggctac
    29881 cactaaaaat gttaagagcc gcaattgcgg ctcttttttc attctgccgt ttcagtctct
    29941 acggcttcat ttttggcatt gcgcttcatc cacaacgcca gcgctttcag cgagtctgga
    30001 gtgaactcat cgcagcgtgc ggtgatttct tccggcgtca gccagcaaac ttcactgact
    30061 tcatcttcct gtagggcgaa gggaccgtga gagacgcagc tgaacaacgc gccccagaca
    30121 cggcaatttt tatcttcgaa atagaactgc ccgtgctcgg caaaggggac accggcaatg
    30181 cccaactctt cttccgcttc gcgacgcgcg gattccagca gttgctcatc ggcctgaacc
    30241 acaccgcctg cggtcgcatc taacatgccg ggtaaaaagt cttttgtctc ggtacgacgc
    30301 tggaccagaa ttttgcccat gccatcatgc acgacgatgt aagttgcacg atgacgcaga
    30361 cactgtgccc gcatttgttc ccggctggct tgtgcaatga cttcgttctc ttcattgaca
    30421 atatccaccc attcagtact tgccaaacga cgctgttcca ttatcaggaa accttctttt
    30481 tctggcgctc ttacggcgca ttttgaattg tgggtaaatt acggattaat cgcgacctgc
    30541 gcaatgatac tttgatcatt gagtgcgatt acactaagta cgtcattatc cagcatgcca
    30601 taactcgccg gattaccgcc tttcggaata ctcaccgagc cggggttgaa gtggaaaatc
    30661 tccccccgct gttctgccac tggcagatgg gtatgaccgt acaccagcac atcgttctgg
    30721 tttaaagcag gtagattttc cgggccaaaa agatgaccat gcgtcaaaaa cagacgttgt
    30781 ttttccagta atacctgttg ccacggcgcg gttatcggga aatgcagcag catttgatcc
    30841 acttcgctgt cgcagttgcc gcgcacagcg ataaccttat gtgccacttc attaagccgt
    30901 tcagcgactt tggctggcgc gtaaccctcc ggtaaagcat tacgcgggcc gtgattcaac
    30961 acgtcgccaa ggatcaccag ccactgggca ccgctttggg caaacaactc cagaacacgt
    31021 tccgtcgccg gtaacgaccc atgaatgtcc gatgcaaaca tcagtttcat cactcactcc
    31081 tcgtcgaaaa aacgtaagct atgatactgg attctgtcgt tgctatcagc cagattgctt
    31141 cgccgaaagt gcaataaaac gctggacaga cgctcgctgc cactggaatg tcgcataatc
    31201 caccaggcgt tctggcacct catcgccatg tagtaccagg cggttaatca ttagcgccag
    31261 atcagtatca gcaatgcacc attcaccaaa taaattcggc tgacccagtg ctaacaaatg
    31321 ttctgccatc gcgaacagtt tctctgcact ggcttttccc tcggccgtta gtggcgcttt
    31381 tttcgccccc gcaaagacaa catccgtcgg acgctcttcg cggatgggca tcagatcgct
    31441 gcgcagccag gcctgaatcc gtcgtgcacg cgcacgattt tctaaatcaa gcggataaat
    31501 acgttcccag gtcggtggcg caaatcgatc ttccagatac tccgcaatgg cagaagattc
    31561 actcaactca aaatcatcga tttgtaataa cggcacacgg cgtgtctgac cgtaaccttg
    31621 ccacgtcggc ttcaaatgtt caccgctgtc gaggtcgatg gtcttgatat gaaacgacag
    31681 gcctttttcc tgcaacgcca cccaggcgga taacacataa ggggagaaaa agtgggcatc
    31741 tgaccaaagc gtgatagcgg gtttactcat tatgtcctcg gcaataaagg gggctggcta
    31801 tcaaactata gagccttttt gtcactgtca cgtgacgaat actcacgtaa gtcgccgcgc
    31861 tttctatact tgtggtgttc agatgattaa acaaccggag ctgcaatgat cgatctctat
    31921 ttcgccccga cacccaatgg tcacaaaatt acgctgtttc tcgaagaagc agagcttgat
    31981 tatcgcttga ttaaggtaga cctggggaaa ggcggtcagt ttcgtccgga atttttgcgc
    32041 atttcgccca acaacaaaat tccggcaatt gttgatcatt ctcctgccga tggcggcgaa
    32101 ccgctaagcc tctttgagtc tggtgccatt ttgttgtatc tggctgagaa aacaggactc
    32161 tttttgagtc atgaaacgcg tgagcgcgcc gccacattac agtggttatt ctggcaggta
    32221 ggcggactgg ggccgatgct tgggcaaaat catcatttta atcacgcagc cccccaaacc
    32281 attccttacg ctattgaacg ttatcaggtt gaaactcagc gtctttacca tgtactaaac
    32341 aagcggctgg aaaactcgcc ctggctggga ggcgagaact acagcattgc ggatattgcc
    32401 tgctggccgt gggttaatgc ctggactcgc cagcgaattg acctcgcaat gtatccggca
    32461 gtcaagaact ggcatgagcg gatccgttcg cgccctgcca ccgggcaggc actgctaaaa
    32521 gcacaactcg gtgatgagcg ttcggatagt taacagaaac aggttctcgt gtattatttc
    32581 atcctaagta aaacaacgga gaacctgcaa tggcacaacc tgccgctatt attcgtataa
    32641 agaaccttcg tttacgtacg tttatcggaa ttaaggaaga agaaattaac aaccgtcagg
    32701 atattgttat caatgtgacg atccactacc ccgccgataa agcgcgcact agcgaagata
    32761 tcaacgatgc gctgaattat cgcaccgtaa cgaaaaacat tattcagcat gtagagaata
    32821 accgtttctc tttgttggaa aaattaactc aggatgtgct cgatatcgca cgtgaacatc
    32881 actgggtgac gtatgctgaa gtggagatcg ataaactgca cgcgctgcgc tacgccgatt
    32941 cggtatccat gaccttaagc tggcagcgtt aaccgccata tcgggaggct gcatgaacat
    33001 agtgatcacc ggagggacgg gattaattgg tcgccatttg attccgcgtt tgctggagct
    33061 gggccatcaa atcacggtag tgacgcgtaa cccacagaaa gccagttccg ttctcggccc
    33121 tcgggtgacg ctatggcaag ggctagccga tcaaagcaac ctcaacggcg ttgatgcggt
    33181 aatcaacctg gccggagaac cgattgctga taaacgctgg actcacgagc aaaaagagcg
    33241 tctctgccaa agccgctgga atatcacgca aaaactggtc gatttgatta atgccagcga
    33301 cacgccgccg tcggtactca tttccggctc ggcaacgggc tattatggcg acttaggtga
    33361 agtggtggtt accgaagagg aaccgccgca taacgaattt actcataaac tctgcgcccg
    33421 ctgggaagag attgcctgcc gggcgcaaag tgacaaaacg cgagtgtgcc tgctgcgaac
    33481 cggcgtggta ctggcaccgg acggcggtat tctcggtaaa atgctgccac cgtttcgtct
    33541 tggcctgggc gggccgattg gttccggtcg gcagtatctg gcctggattc atattgatga
    33601 tatggtcaac ggcattctct ggctgctgga taacgacctg cgcgggccat ttaatatggt
    33661 ttcgccctac ccggtacgca atgaacaatt tgcccatgcg ctcggtcaag cgctgcatcg
    33721 cccggccatt ttgcgcgtcc ccgcaaccgc cattcggctg ttaatgggcg aatcttcaat
    33781 actggtatta ggtggacaac gcgcgctgcc taaacggctg gaagaagcgg gttttgcgtt
    33841 tcgctggtac gatttagaag aggcgctggc ggatgtcgtt cgctgatgtg gtttacagca
    33901 aacatccgcc agttaactcc cggtgttaca ggattagtgg ctttgcgcga taagatcgtc
    33961 tggtgaaagt cgggtcacca tcataactaa ctctctgtct aaacctctat ccagcatctc
    34021 ctgagcaata cgcagggctt cttcgtgttt gccctgcatt gcgccttctt cacgtaatct
    34081 gtcagcaatg gtcatcagtt tctccttttc ttgtggtgcg cgttctgtta tctcaccaat
    34141 aaatgcacgg aaacgctggg catcccctgt ttgtaatacg taattaaaca gggcttttag
    34201 ctgtctgtca ttagtgttcc ctgtaactag cagcgaaaca atttggtcga ctaatcccaa
    34261 cagatcgcgc tgacgaatat gtttctgaat taactccaac agcgccattt tgcggtgttg
    34321 cataatctcg tcatccggca ccacggtaat atccaccaac ggaaacgctg atgaatatat
    34381 tttgcgggct atagcaggct cggcaaattc atcaagccag cagagtgaat aaggataagg
    34441 acttctgcaa ccatgataaa acagcatcgg gatcaccaat ggaagctctt tatagcccgc
    34501 atcaagatgg ttttgcattg ccgcaatgga ataacgcatc atgcgaaaag ccattaattc
    34561 ttccagctta ctttggtgct ctatcactac ataaatataa cccactccct cctgcgtttt
    34621 cacagaccac aagaggtcgg aataatattg ccgcaggtct tcatcaataa aactgtttga
    34681 ttctagttta agcgtcgtta aatcacacag tttgcgcagc ggcgcgggaa gatgaatatc
    34741 aataaaatcc cgcgcggtgt ctggatggcg taaaaaagat ttaaataccg catcatgcgg
    34801 cgtggaagtt gtcgatattg tcatggcgtt ccgtcacccc tcaaatttaa gatgacgcga
    34861 caataaccac gattaacacc caatgcagca ggcagttttt atctttggac agcgaattca
    34921 gaagtatttt tagcggactg caacttttac atttcagcgg aagctgactc gcaaattccc
    34981 catgaaaata ctccggcagc gttagccacc ggagaggaaa ttatttcagc gatcccttaa
    35041 ggaactgttg cagacgaggg ctttgcgggt tgccaaataa ctgctccggc gcgccctctt
    35101 cttctatttt cccctgatgg aggaaaatga cgtgagtaga aacatggcga gcaaagccca
    35161 tttcgtgagt cactaccacc atggttttcc cctcttctgc cagttgctgc ataatacgta
    35221 acacttcgcc taccagttca ggatcgagcg ccgaggtagg ttcatcaaac agtaaaactt
    35281 ccggttccat cgccagcgcc cgcgcgatag aaacacgctg ttgctgaccg ccggaaagat
    35341 gcaccggata tttcccctgc gcacgttcgt ctatcccgac tttcgccaga tacttcaccg
    35401 cccgctcgcg cgcttcctgc ttgctcaggc ccaacacctg aatcggcgct tccatgacgt
    35461 tttccagcac cgtcatatgg ctccagagat tgaagtgctg gaataccatc gtcaggcgtg
    35521 tgcgcagtaa gcgcagttga tttttatcgg cgactttgag ttgaccgtct ttgtcgcgca
    35581 ccagattgat cgtctggcca ttgaccacga tcgacccttc actcggtttt tcgaggaagt
    35641 taatgcagcg cagaaaggta cttttccccg atcccgacga tccgatgatg cttattacat
    35701 ctccggcatt cgcttgcagt gagacccctt tcagcacttc atgttcgccg tagcgtttgt
    35761 gcaaatcgat aacgtttaat ttattctcgg acatcgtatt ctcagtgcgt tgaagaaggt
    35821 ttcacatgct gcaaccagcg tttttccgct ctgcgaaaga ggctgatcag gacataagag
    35881 atgattaaat agagcaccgc ggcaatgccg aaggcggtaa aaggttgata cgtggcggcg
    35941 ttaatatcgc gggctatttt cagcagatcc ggcacagtgg ccgtaaacgc cagcgcagta
    36001 gagtgcagca tcaggatcac ttcgttgctg tatgccggta acgcaatacg cagcgcagaa
    36061 ggcaaaataa tgcaacgata cattttaaaa gtcgagaagc cataggctct ggcggcttca
    36121 atttccccat gcggaaccga acggattgcc ccagcaaaaa tctcggtagt gtaagcgcag
    36181 gtgttaagcg tcagcgccag cacggtacag ttcaggccac tgcggaagaa agcgttaagg
    36241 aattcggttc ccttaacaat ctcaagcgtg tacatgccgg aatagaacac cagcaactga
    36301 acatacagcg gcgtaccgcg aaaaatatag gtaaataacc agattggaaa ctggatgtat
    36361 ttattactgg agacacgacc aatcgccaga aacagcgcca ggactccgcc tatcactacc
    36421 gacaaaataa gcagccacag agtgatcgcc acaccagtaa agcgataacc gtcggtccac
    36481 agcagcggtt tccagtattc atgtaagatt tcgatcacag gtcagccctc ttcacaccca
    36541 cggagtagcg gcgctcaagg aacagcagca caccattgga aacagtggtg aaaaccaggt
    36601 aaatcacgcc acagacgatg gcgaaataga acggttccca ggtacttttg cctgccagtt
    36661 gggtggcttt gaccacatct tccaggccga gtaacgaaac cagtgcggta gatttgagga
    36721 tcacctgcca gttgttgcca atgcctggca gggcgtaacg catcatcgcc ggaaacatga
    36781 tccgccgaaa cacttgccca cgagtaaaac cgaacgccgt cgccgcctct atatgtcctt
    36841 tcggcactgc cataaaagca ccacggaacg tttcggtaaa ataagcaccg tagataaaac
    36901 cgagagtgat aataccagcg accatcggat cgatatcaat ctgcccgacg cccatcgcct
    36961 ccgtcaccgt gtttagcgca atctgcaaac cgtagaaaat cagcagcatc aacactaagt
    37021 ccggcacgcc acgaatcagc gtggtgtacc cttcgaaaat cagccccgaa agccgatttt
    37081 gcgagagctt accgccagcg ccaattaaac cgatgattac agcgagcact acagagctga
    37141 tagccagctc cagcgtgacg agcgcaccct gtaaaataac acctgaaaac ccatacaaca
    37201 tgctgcctgt cctgtcgtgt gtggtgaatt accgcctctt gtctccctcc ggttacccga
    37261 agggagcggg ctttctttca ccgatggcga ttagccacca taaacatcaa aatcgaagta
    37321 ctttttcgct aatttctcgt aagtaccgtc agcgcgcatt tcggcaaagg ctttgttcag
    37381 tgcttcgcgc agttcgttat cttctttacg caggcccatg ccggtcccta cgccaaacag
    37441 tttttcatct ttaacagacg ggccaccgaa tttgtaatct ttaccgacag gttgtttgag
    37501 gaaaccttcg ctggcagcga cctcatcctg gaacgcggca tcaatacgtc cggcagtcag
    37561 gtcagaataa atgttgtcct gcccctgata cgagacgatt tcaatgcctt ttggtgccca
    37621 atgttcatta ccgaacgtct cctgggtggt gccctgcaat acgcctaccc gtttgccttt
    37681 cagcgactcg actgtcggct gaatgtcaga atttttcgcc accaccaaac gagaatcggc
    37741 agcgtacagt ttgtcggtga aggctatttc ttgctgacgt ttttccgtaa tggaaagcga
    37801 tgacatgatg gcgtcaatct tcttcgcttt taaggacggg attaacgcat ccagcggatt
    37861 ttcgacaaac gtacattgcg tattgatgcg tttgcataat tcctttgcca gatcgatatc
    37921 gaagccaacc agttcgcctt gtgaattttt tgattcaaat ggcgcatagg tcgggtcggt
    37981 accgatgcgg atgttttgcg gaatcgcagc aaatgccgca gttgcgctgg agaaggccag
    38041 aaccagagag agcgatagca ccagtttttt cataatgtcc tcaactgaca gtctttttat
    38101 agggaatatt tacaggtttg ttacacttat cgtgccataa aattgaccat caaggcaaaa
    38161 tattccgccc cgttagacgt attatttttc attaaaaatg cttaagtatg catttcaatg
    38221 caccacgata gtgcaacaaa cctagcgtgc accatgatga tcattccatc aggtacagct
    38281 tcccagcgac gtatcagtca ccgtagacat taaagtcgaa atactttttc gccatcttgt
    38341 cgtaggtgcc gtcctgacgc agctcgccaa gcgccttatt gaaggcagcc gtcagttcag
    38401 catcatcttt acgtagccct acaccggtgc catcaccgaa gtattttttg tcttttactg
    38461 atgagccagc aaaggcgaaa gctttaccag caggttgctt gaggaatcct tcgctggcag
    38521 caacttcatc ttgtaacgca gcatccagac gtcctgcagc cagatcggaa tagaccaaat
    38581 cctggttggc ataggccacc acatccacgc ctttgctacg ccaggtctcg ttagcgtaag
    38641 cttcctgggt tgatccctgc agcacaccaa catgtttacc tttcagtgaa tccagcgttg
    38701 gctgaatcgg tgaacctttg gcagcaatca aacgagaatc tgcggcgtac agcttgtcgg
    38761 agaaggcaat ctcctgctga cgtttatcgg taatggaaag cgacgaaata atggcgtcga
    38821 tttttttcgc tttcagtgag gggatcagcg cgtcaaagtc actggcaacc caggtacatt
    38881 tcacctgcat ccgtttgcac atctcgttac cgagatcgat atcaaagcca acaaaatcac
    38941 ctttagcatc tttcgacgag aacggcgcat aggtggtatc ggttccgata cgtaccgtct
    39001 ccggtagcgc cgcatagctg gaagccgctg cggagagacc gactaacaaa gacagagcga
    39061 gaatcgactt cttcatacat aaccctcaag tgaaaatggc ttttttatgt tgtgtattgt
    39121 gttgtgtgtt tgcaggctct ttcatgcagg tcttatgcca tcttgacgac aacagtaaca
    39181 ttcaacgtta aatatgttaa taagacgttg cattattgtc ctgaagttga agatagcagg
    39241 tatagcggtt gaatcgcagc gtttcgattg aatggcagaa acaaaatgtc gaggatttga
    39301 tcgcggttgc aaaattgccc tgaaacaggg caacagcgga gttatgcgcc ctgccagcgg
    39361 gcaaagagat cttcaggaag ggttattgca aactggtcaa gaacacgatt aaccgtctga
    39421 tttatcacat catcaaggga ttgcgggcga tgataaaacg ccggaacggg aggcataatc
    39481 accgcaccga tttctgccgc ctgagtcatt aaacgcagat ggcctaagtg caatggtgtt
    39541 tcacgcacgc agagcaccaa cgggcgacgc tctttcagca ccacatctgc cgcacgggtc
    39601 agtaagccat cagtatagct atggacaatg ccggaaaggg ttttgattga acagggtaaa
    39661 atcaccatcc ccagcgtctg gaaagaaccg gaagagatgc tggcggaaat atcgcgcgca
    39721 tcgtgcgtga catcagctaa tgcctgcact tcgcgcaggg aaaaatccgt ttcgagggat
    39781 aaggtctggc gcgccgcctg gctcatcacc agatgcgttt cgatatctgt gacatcgcgc
    39841 agaacctgta ataagcgcac gccataaatc gcgccgctgg caccgctgat gcctacaatg
    39901 agtcgtttca taaatagttg ccctgtcaga cttgcaggca gactttgcag tatttcggcg
    39961 ggagttgcaa gtcagggtgc cagaccggca ccctcagcga aggcatcatc cttcgttatg
    40021 catttcgaga ttttccactt cgttctgacg ttgcactgct ttggcgtcat cattacgtaa
    40081 cgtatcgagg aaatcgaggt agccctgatc aacatctttg gtgacgtaga cgccgttgaa
    40141 caccgagcat tcaaactgct ggatatccgg attttcagcg cgaacggcgt cgatcagatc
    40201 gttcagatcc tggaaaatca acccgtcagc accgatgatc tggcgaattt catcaacttc
    40261 gcgaccgtga gcgatcagtt ccgtggcgct cggcatatca ataccataaa cgttcgggaa
    40321 gcgaatttcc ggtgccgcag aagcgaggta cactttcttc gctccggctt cgcgtgccat
    40381 ctcgataatc tgctcagaag tggtgccacg gacgatggag tcgtcgacca gcaggacgtt
    40441 tttatcgcgg aactcggcgc ggttggcatt cagtttacgg cgcacggact tacgacgcag
    40501 ctgctggccc ggcatgataa aggtgcggcc aacatagcgg tttttaacga agccctggcg
    40561 gtacggtttg cccagaatac gagcaatttc cagcgcgata tcacacgagg tttctgggat
    40621 cgggatcacc acgtcgatat ccagatcttc ccattcgcgg gcaattttct cgcccagttt
    40681 cgtgcccata ttcacacgcg cgctgtaaac ggaaattttg tcgatgaacg agtccgggcg
    40741 agcaaagtat acatactcaa acaggcacgg attgctgacc ggattgtcag cacattgacg
    40801 ggtaaacaac tgcccttctt cagtgatgta aatcgcttcg cccggcgcga cgtcacgcag
    40861 gaaatcaaag cccagcgtat cgagcgctac gctttcggaa gcgaccatat attctgtacg
    40921 gttctcgtca atatcacgtt ttcccagtac cagcggacga atcccgtttg gatcgcggaa
    40981 agcaaccata ccgtggccga taatcatcgc cacacaggca tatgcgccgc ggattaagcg
    41041 gtttgtggca gcaatggcag cgaaaatatt gtcggcttcc agcgggtagt ggcggaagtt
    41101 gtccagctcg ctagcgaaga tattaagcag aatttccgag tcggaagtgg tgttgatgtg
    41161 gcggcgtttt tcttcaaaca gttttttacg caactcgtga gcgttggtca gattgccgtt
    41221 gtgggcaagc gtaatgccat acggggagtt aacgtaaaac ggctgcgctt cagaggcgct
    41281 ggagctgcca gccgtagggt aacgcacatg accaatgccc atattgccct gcaaacgctg
    41341 catatggcga gcttcaaata catcgctcac cagcccgttc gctttacgca aacggaagca
    41401 gttattggca tctatggtga tgatgccggc ggcatcctga ccgcgatgct gaagcaccgt
    41461 taaggcatca taaatcgact ggttaaccgg cataacaccg gcgataccga caataccgca
    41521 catacgtctt tttcctcgtt aagccacatc tcagagcact tacgctctgg gcaagaaact
    41581 tgacgagctt tgcagataat caaaaaacca tctgatgata aaactgaact gcgggatcag
    41641 ctgtgatttg ctccagtctt cgcttttcga caccccggta aaggagtcga gaaagaagag
    41701 aatggcggca acaatcaaca caccgcgcaa cgcaccgaaa cagacgccca gcacccgatc
    41761 ggtgcctgac aaccccgttt tctccaccaa ctggcctatc acgaagttca cgatagcacc
    41821 aacaatcagg gtagcgataa acagtaccgc gatggcaatt ccatttcgaa ccagttcgtc
    41881 ttcaaagccc gtaaaccaga ctgacaggta agtgtagtaa tgactggcaa caaagaaagc
    41941 acaaccccat gtcaccagcg ataacgcttc acgaacaaag ccgcggatca ggctaaccag
    42001 agaggaaaaa gcaatcaccg cgattatggc gtaatcaatc cagaccatat gtgtcccacg
    42061 attttacgcc ctgtcatcct gttcggggcg cattctaaca gaaaaagaaa acgtttgcgt
    42121 agggatttcc ttcccacgca tcaataaaaa tggcgctgaa aaaatattca acgccatcga
    42181 ctttttatgc ctttgcggca tcgtgcaatg cgtgtcggat gcggcgtaaa cgccttatcc
    42241 gacctacggt tctaacactg cccaggcctg ataagacgcg ccagcgtcgc atcaggcaag
    42301 actgtattaa ttcggcgtat agcccattac cacgccactt aagccagaaa gttgcttcaa
    42361 ctcacccagc gaacctttca gcttatcttt cgaggcatcc ggcccaacca gaatacgggt
    42421 aattttaccc tgcactggcg tggatggcga cgtataaacc cgataaccgg caccgcgcag
    42481 cttaccgaca atctcattca ctttatcggc atttttcagc gcacccagtt gcacaacata
    42541 ggctttaccc gttggcgctg ctttttcttc cacgaccggc ttcggttctg gcttcggcgc
    42601 aggtggtgct tcaaccttcg gtttaggcgg ctccaccggt ttcggcttcg gtggggcgac
    42661 cggtgcaggt tccggttcaa actcggtgtt attggctgca atagtggccg gatcgagcga
    42721 cggtgctgcg gcatcacctg cccgcacctc ttccgctgcg ccttccggcg gctgcgtcgg
    42781 caacgcctgg gtggcggctg gcatcatatc aggctcatcg cgatcgcccg cttttggaac
    42841 cagcgggata gccgcaaact catcctgata atgttttttc tgcccgtcca gcagccctgg
    42901 aagtacaatc acccccagcg ccaccagcac gatcgtgccc actaaccgat tctgaaactt
    42961 acttgccacc gcttctcctc gcgtcaatca cttccatgac atgtgcgacc gtgtggaaag
    43021 aaccacacac cagcacggtg tcttccgctt tagcgtccgc cattgcggca tcccatgcct
    43081 gcgcaacgct atcaaatgat ttgccgttac ccaaatgctc aagcagttgt tctgccgtgg
    43141 caccgcgcgg cccttccagt ggcgcacaat accagtcatc aaccacgctt ttcaaccagg
    43201 ccagagttcc ggcaatatct ttatcatgta gcataccgat aaccgccagc acgcgcccgt
    43261 ttttcggtag cgctttcata cgcccggtga gatattccgc cgcatgtgga ttatgcgcga
    43321 catcaaaaat aacgcgtggc gactcgctca caatctggaa acgtcccggc aaaattgcgc
    43381 tggcaatccc gtcgcgaatg gcattttcac tgacttccag cccgctggca cgcagtgccg
    43441 ccagcgctgt tgcggcattc ggttgcggga caagcggcaa cggcagattt tccagcgtgc
    43501 cgtgagcatc gctaaacgcc cagtcatgat cggtgacgga atagttccac tcaacgcccc
    43561 gacgttgtaa cagtgcacct ttttcctgcg ccacatcagc aatggtagaa ggcatttccg
    43621 gctcaccgac aattgccggt tttgcgctgc gaaagatgcc tgctttctcg cgaccaatac
    43681 tttcgcgatc tggtcccagc cagtcggtat gatccagcgc aatgctggtt actacagcca
    43741 catcggcatc gacaatattg gttgcgtcca gacgaccgcc cagccctact tccagaatca
    43801 ctacgtcaag ttgtgcctgt ttgaacagcc acaacgccga cagcgtaccg tactcgaaat
    43861 aggtcaggga aatatcaccg cgtgccgatt caatctccgc aaaagaggcg gtgtgggccg
    43921 attccggcaa ttcctgcccc tgcacacgta cgcgctcggt ataacgcacc agatgcggtg
    43981 aactgtagac gcccactttg taccctgccg ccatcagaat cgactccagc gtacggcagg
    44041 tggtgccttt gccattcgta cccgcaacgg taaacacaaa tggcgctggt ttcaggacgc
    44101 caagacgcgc cgcgacctgg ctcacgcgct caaggccgag atcgatagtt ttactgtgca
    44161 ggttttccag ataagaaagc cacgaagcca gaggcgacgc ggcttgagga gtgcgtttga
    44221 taatcatggt atccgctgat tcgttacggt gagaatagca aaagggcaga gccagtggcc
    44281 ctgcccttat cagttatcag gcctcaggtt cctgatccgg taccgggggt accactacgc
    44341 cttcacgcgg cgcttcagga ttcggcgctg gcagattcat caacttcgcc agaatgctcg
    44401 ccagtttcag gcgcatttcc ggacgatgga cgatcatgtc gatggcgcct ttctcgatca
    44461 ggaattcact gcgctggaat cctggcggca gtttttcgcg aacggtctgt tcgataacac
    44521 gcggaccggc aaagccaatt aacgctttcg gttcagcgat gttgagatcg cccagcatgg
    44581 cgaaacttgc agaaacaccg cccatcgtcg ggtcggtcag cacggagatg tacggcaagc
    44641 cgcgctcctg catttttgcc agtgccgcag aggttttcgc catctgcatc agcgacatca
    44701 gtgcttcctg catacgtgcg ccaccagagg cggagaagca gatcagcggg cagttatctt
    44761 ccagcgcctg ctcaacggca cgcacgaaac gtgcacccac aacagacccc attgaaccgc
    44821 ccataaaggc gaactcgaat gccgcagcga caaccggcat tccatacaga gtgcctttca
    44881 tcaccaccag cgcatctttt tcgccggttt ctttctgcgc agaagccaga cggtctttat
    44941 acttcttgga gtcacgaaac ttcagcacat ctttcggctc aagctcgcta cccagctcca
    45001 caaggcttcc ttcatctaac aggctatgca ggcgattacg cgctgtcata cgcatgtgat
    45061 ggtcacactt cggacagacc tcaagattac gttccagctc agcgcggtat aaaacctgac
    45121 cgcagctatc acacttagtc cacacccctt caggaatgct cgccttgcgg gtgggagtaa
    45181 tgttgctttt aattcgttca atccagctca ttagggacct ttctgtctga acctggttcg
    45241 atgccagttt tatctttggg gacgcataat gccatttttg cccccaacag accatgaatg
    45301 ttgcacatta aaacataaca gcccgaaact ttggataaaa aagtggtcga accgcggagt
    45361 tactttttat tttgcggcgc gtgccgcagc gcgtttgtga cgaataaatt cgaaaacccc
    45421 aggcaatatt gaaacaaaaa taatgccaac aatcatcagc ttaaggttgc tctgaatgaa
    45481 ggggagcgtg ccgaagaaat aacccgcgta ggtaaaaatc agtacccaca acagtgcgcc
    45541 gatcacgtta taagcggcga aatgacggta cgacatgtgg cccattcccg caacaaacgg
    45601 cgcgaacgtt ctgacgatcg gtacaaaacg ggcgagaata atcgttttgc cgccatgtct
    45661 ctcgtaaaac tgatgagtct tatcaagata actgcgacga aagattttcg aatccgggtt
    45721 actgaacagt ttgtcaccaa ataaccgtcc aatggtatag tttactgcat caccgacaat
    45781 agcggcaatc aacataagca ccaccatcat atggacattg agatcgttag tttccagcga
    45841 cgccaatgca ccagcaacaa acagcagcga atcaccaggc aggaatggcg ttaccaccag
    45901 ccccgtttcg cagaacagaa tcaaaaacag aatggcataa acccagacgc cgtactccgc
    45961 gaccagctct gccagatgca cgtcaatgtg caggataaaa tcgatgagga aataaatcag
    46021 gtccatattt gcctatgcct tgtactcgtc atctttcagg ctgtaactac gttggctaca
    46081 gcctgaaatg ttccgagcat tattctcgtt agtccgccag aaatagcggg cccattggcg
    46141 gttttggaag atcataccgg tcagggtaat ccaccgcgac cagatacagc ccttccgctt
    46201 ttgccgttgc tgccgccagc gttctgtcct ttgccgccag caactctgct atccatctct
    46261 ccggctggtt gtgggcaccg acttccatca ggctgccgac aatattcctg accatatgat
    46321 gtacaaaggc attcgctttg atatctacca ccacataagg accgtgacgc gtgacattaa
    46381 tatgcataac gttgcgccac ggggttcggg actggcactg caccgcacgg aacgaggtga
    46441 aatcattctc gcccagcaag cattgcgcag cccgatgcat ccgttcagcg tccagcggtt
    46501 cgtaaaaatg ggttacccct ttactcaata ccgccgggcg cagccgatga ttgtagatga
    46561 tgtagcgata acggcgagcc gtggcgctaa atcgggcatg aaaatcatca ggtacagctt
    46621 taacccaacg cacggcgatg tcaccaggta aattcgcatt tacgcccaag gtccacgccg
    46681 cgtctttgcg ctgcgcggtt gtttcgaaat gcaccacctg cccggtaccg tgtacccctg
    46741 cgtcagtacg cccggcgcag aagacggtga tgggttcgtt cgccacctgg gagagcgcct
    46801 tttccagctt ctcctgcaca ctgcggactt cattctgccg ttgccagccg taatacttac
    46861 tgccgtcgta ctcaatgccc agcgcaattt tataaactgg cagttgttgc tggtcggaca
    46921 ttagtacaga tactcctgca ccagtttctc ggcgattttt actgccatca gcgcgccgcc
    46981 aaagcgaacg ttatcggcca ccgaccagaa ctggacttgc tccggcatac cgtagtcatt
    47041 acgcacgcag ccaacagaaa gatgcggcgt acccgaagca tcccctacct gagtcgggaa
    47101 ttcgttctct tcagagagca caatatcttc gccctgagca aacgcatcac gcgcttcttc
    47161 tgccgccagc ggacgcagtg cttcaaagtt gaccatctgg gcatgaccgt agaataccgg
    47221 tgcctggacg acgcttgccg aaatcatcag cccttcgtcc tgcaggattt tgcgtacttc
    47281 gtcaacgata cgacgttctt cacgcacgct accttcgcta tccggcaaca acggcagcat
    47341 attgaacgcc agttgacgac cgaagaaatc ttcttcgtca atcggaatgc cgttgagcaa
    47401 tttcgcactc tgccccgcta acgcatcgac cgcttttttg ccctgggcgg aggctgaaat
    47461 caggctggta acactgatac gcgacaaacc gccctgatcg attaacggtt tcagtgccgc
    47521 cagcagctgg ctggtcagac tgtctggtac ggcgatgaca ttccggttgc ggtaatcggt
    47581 cagtacaaac gggtttactt ccggcaccac cagcggtacg tcgggttcga gagcaaacag
    47641 tccgctgctg tcgatcacca ggcaacctga gttggtcgct tcttcaaccc aggcagcggt
    47701 agcttctttg cctgcgacaa aaaatgccag ctgcgcctgc gtccagtcga attcagcggc
    47761 atcctgcaca gtgattgttt taccaccaaa gcgcagttgt tcgcctgcgc tttcgttacg
    47821 tgccagtgca taaatttccc caaccgggaa ctgacgttca gccagcgttt caagcagggc
    47881 ttcgcccaca gcgccagttg cgcccaggac ggcaatgttc cagccttcag acatggtggt
    47941 ttactccaga aatagcaaag ctccctgcca atgttacagc agagagcatg aagaagagat
    48001 taacgtgccg gatgatgaac ggcgttaaaa cccagtttac acagcaatga tgccgcactg
    48061 gcgtcatcac aaattacata cagagatgac cattcacggc gctcaagata gtttttgcgc
    48121 agtttatcga actcacccgg tatcccggcg actttacgca gcggtgcgtc atcgcggcgc
    48181 acatcataca ccaaatgcac cagccttttc agcgtcggtt gatcgagcgg gccatgcagc
    48241 gtaatgcgac caaactctgg cgcgggcagt aatgtatcca gcgcaacgtg ctgttcatgc
    48301 ccaataaact tgctataggc ttcaaacact tgcgtagtac cgcgtgcttt accttccagg
    48361 gtataacctg cgatatgcgg cgtgccgata tccacttttg tcagcaactc cacgttaagt
    48421 tccggttcgc cttcccagac atccagcact acgcttaact tctggccttc attcaggcag
    48481 gtcagcaacg cagtattatc gacgactgcg ccacggcagg cgttaatcag aatcgctccc
    48541 ggcttcaggc tacggatcag tttttcatcc gccagatgca gcgttttgta cggcccatct
    48601 ttgaagagtg gcgtatggaa agtcagaata tccgcatgct gaactaattc atccagcgag
    48661 cggaaatcac cctcatcccc acggtcagcg cgaggcggat cgcaaagcaa tgttttaatc
    48721 cctaacgctt ccagtcgcgc ctgcaatcga cgtccaacgt taccaacgcc cacgatcccc
    48781 accgtacggt cgtgcagtga aaatccatcg cgttcggcaa gcatcagtag tgaggagaaa
    48841 acatattcca ccaccgcaat cgcattacag ccaggtgcag cggaaaaacc aattcccgcc
    48901 tgcttcaacc atgcttcatc gacatggtcg gtccccgcag tggcagtgcc aacaaattta
    48961 atgggttttc ctgccagcaa agattcattc actttcgtga ccgaacgcac catcagcgca
    49021 tccgcgtctg ccagttgagc gacggggatt ggacgcccgg gaaccgcggt cacctcaccc
    49081 aaacggctaa ataagtcgcg ggcataaggc atattttcat caacaaggat tttcacgttt
    49141 gtgttacctg tatgagacga gagttaaccg gacaagtgtg ccataatctc gcggccaggc
    49201 atacttgcga agatttcagg tataaggata cgtaatgata caacctattt ccggccctcc
    49261 tcctgggcaa ccaccaggtc agggagataa cctgccgtct ggcgcgggca atcagccttt
    49321 atccagtcag caacgtactt cgctggaaag cttaatgacg aaagtgacct cactgacgca
    49381 acaacaaaga gcagaactgt gggcgggtat caggcacgat attggtctgt cgggagattc
    49441 accgctgctt tcgcgtcact tccctgccgc tgagcataat ctggcgcaac gtctgctggc
    49501 cgcgcaaaaa agccattctg cccgccagct tttagcgcaa ttaggggagt atttacgtct
    49561 ggggaataat cgtcaggcgg tcacggatta tatccgtcat aactttggtc agacgccgct
    49621 gaatcagctc tcaccggagc aattaaaaac cattctcacc ctgttgcagg aagggaagat
    49681 ggttattccg caaccacagc agcgcgaggc gaccgaccgt cctttattac cggcggagca
    49741 caatgcgcta aaacagctgg tgaccaaact tgcggcggca acgggggaac ccagcaaaca
    49801 gatctggcaa tcgatgctgg aactttccgg ggtgaacgat ggcgagttaa ttccagcgaa
    49861 actgtttaac catctggtga cctggttgca ggcgcgccag acgttaagcc agcaaaatac
    49921 gccgacgctg gaatcactac agatggcgct aaaacaacct ttagatgcca gtgaactggc
    49981 agcgttatcg gcgtatatcc agcaaaaata tggtctttct gcgcaatcat cgctttcttc
    50041 tgcccaggcc gaggatattc ttaatcagct ttatcaacgg cgggttaaag ggattgatcc
    50101 gcgtgatatg caaccgctgc ttaatccttt tccaccgatg atggacacgt tgcaaaatat
    50161 ggcaacgcgt cccgcgctgt ggatactgtt agtcgcgatt atcctgatgc tggtctggct
    50221 ggttcgttag ccccgacgaa atgacagtat cgtgacaata attcccagca ccgcagagat
    50281 cgccccggca agaaataccg aagagtaacc aaacgtggtc gccagcattc ccgccagcgg
    50341 cccggagacg ccgagggcga tatcctgaaa cgcggcgtaa ccgcccagtg cggtgccgcg
    50401 aacttgtgag gggacgcgtt taaccacctc cacgcccagc gcaggaaaga taagcgaaca
    50461 tccggctccg gttaacgccg cgcccgctaa tgcgacccac gcacctgggg cttgccagag
    50521 cagcaacaag cccaccgttt ctacaagcag agagacaatc gccactttca cgccgccaaa
    50581 acggtccggc atccagccaa acatgacgcg catcacgaca aatgcgccgc caaactcggt
    50641 aagagtaaag cccgccatcg cccatccttt gctggcaaag tagagcgaaa cgaaagtccc
    50701 gataaccgca aaaccaacgc cttgtagtgc cagccctaac cctggtttcc agataaaccc
    50761 gacaacgctc cacagcgatg gacgttctcc cgccagggcc ggtactttgc gcactgtgcc
    50821 gttacaggcc cacgccagta agggtaatac cattgtggtg atcgccagtg cggcaaaacc
    50881 gtaatggcta tgaatcaaca ggccaagcgg agcaccaaca gcgagggcac cgtaaatcgc
    50941 cattccattc catgacatca ctttgccaga gtgttttggc cctacgatgc ctaaccccca
    51001 ggtcagagcg cctgtcagta actggctttc accaaaccca agaatcaaac gcccgatgac
    51061 caacagggca aatttgaacg gtgcggagac aggcaaaatc gccgccagca gcaacgcgcc
    51121 gccagccaga ccacaagcta acatcccctg aagcgccgaa cgttttgcgc catattgatc
    51181 ggccagtcgc ccggcatagc cacgcgtcag caccgtagcc agaaactgaa tcccgacggc
    51241 aatgccgacc atggtattgc catagcccag ttcatgatga acaaacagcg ggataaccgg
    51301 caacggcagc cctacggtca tgtaggtgag aaaaaccgca aaagcgatgc ggaagagcga
    51361 aaaattggca gaagatcgtg tttcggtttg gcttacagca gtcatgcatt actccagaat
    51421 gcagcgcaag gcgaggagta tccccgtctc atctctctgg tttcagggtt acggtgcgtt
    51481 ggcaggattt aacgcgtacg tcttttcaga aggaaatcga caaagcggga agtttgcctg
    51541 gaactggtgg cgattgtcaa tgatgtgaaa aagggaacca tcaggttccc ttttgcgtta
    51601 gtgccggagg ccgcattggc gcgtaacgtc ggatgcgacg ctggcgcgtc ttatccgacc
    51661 tactccggcc tactgcgaat taatctttca gcttgcgcat taccagcgtg gcgttggtgc
    51721 cgccgaagcc gaagctgtta gacataacag tggtcagttc gcgatcggtc gtttcggtca
    51781 cgatgttcag acccgcagcc tgctcgtcca gctcttcaat gttgatgctc ggggcgataa
    51841 agccgtgttc cagcatcagc agagagtaga tagcttcctg cacacccgca gcgcccagtg
    51901 agtgaccggt catggctttg gttgcagaaa tcgccgggct cttatcgccg aacacttcac
    51961 ggatagctgc cagctctttc acgtcgccaa ccggagtcga agtaccgtga gagttcaggt
    52021 aatcgattgg ggtatcaacg ccatgcatcg ccatctgcat acaacgtact gcgccttcgc
    52081 cagacggagc aaccatgtct gcaccatcag aggttgcgcc gtagccaacg atttcagcat
    52141 agatgtgagc accacgcgcc agcgcgtgtt ccagctcttc aaccactacc ataccgccgc
    52201 cgccagcgat aacgaaaccg tcacggtgag cgtcgtaagt acgggaggct ttttccgggg
    52261 tgtcgttgta tttagtagac agcgcaccca tcgcgtcgaa ttcgcaggcc atttcccagc
    52321 acagctcttc gccgccgcca gcaaacacga tgtcctgttt gcccagttgg atctgctcta
    52381 ctgcgttacc gatacagtgt gcggaagtcg cacacgcgga gctgatggag tagttaacgc
    52441 catgaatttt aaacggcgtg gcgaggcagg cagaaacgcc ggatgccatc gctttggtga
    52501 ccacatacgg gccaaccgct ttcaggccgc gcgggccgcg cattgcgtca gcgccgaata
    52561 cctggaaacg cggggaaccg ccgccagaac ctgcaatcag gccaacgcgc gggttattct
    52621 ggtaagcttc cggagaaagg cccgcatctg cgattgcctg ctccatagaa aggaatgcat
    52681 aaatggatgc gtcgctcata aagcgcacaa ctttgcggtc aatgaggcca gtggtatcca
    52741 gttttacgtt gccccagacg tggctacgca tgccggaatc cttcagctcc tgagagaaag
    52801 tgatccctga acgtccttca cgcagagatg ccaggacttc ctgctggtta ttaccgatgc
    52861 tggaaacaat gcccaggcca gtaatcactg cacgtttcat tcaatacctc tgtaagtcgc
    52921 acatagagta agtttcgaat gcacaatagc gtacacttgt acgccgaaca agtccgatca
    52981 gccatttaat agagaaattt gcgcagcctt acacacatcg ctaagatcga gccaccgcct
    53041 gtaagacgag taacttacgt gaaacactac tccatacaac ctgccaacct cgaatttaat
    53101 gctgagggta cacctgtttc ccgagatttt gacgatgtct atttttccaa cgataacggg
    53161 ctggaagaga cgcgttatgt ttttctggga ggcaaccaat tagaggtacg ctttcctgag
    53221 catccacatc ctctgtttgt ggtagcagag agcggcttcg gcaccggatt aaacttcctg
    53281 acgctatggc aggcatttga tcagtttcgc gaagcgcatc cgcaagcgca attacaacgc
    53341 ttacatttca ttagttttga gaaatttccc ctcacccgtg cggatttagc cttagcgcat
    53401 caacactggc cggaactggc tccgtgggca gaacaacttc aggcgcagtg gccaatgccc
    53461 ttgcccggtt gccatcgttt attgctcaat gaaggccgcg tgacgctgga tttatggttt
    53521 ggcgatatta acgaactgac cagccaactg gacgattcgc taaatcaaaa agtagatgcc
    53581 tggtttctgg acggctttgc gccagctaaa aacccggata tgtggacgca aaatctgttt
    53641 aacgccatgg caaggttggc gcgtccaggc ggcacgctgg cgacatttac gtctgccggt
    53701 tttgtccgcc gcggtttgca ggaagccgga ttcacgatgc aaaaacgtaa gggctttggg
    53761 cgcaaacggg aaatgctttg tggggtgatg gaacagacat taccactccc ctgctccacg
    53821 ccgtggttta atcgtacggg cagcagcaaa cgggaagtag cgattatcgg cggtggtatt
    53881 gccagcgcgt tgttgtcgct ggcgttatta cggcgcggct ggcaggtagc gctttattgc
    53941 gcggatgaag cccccgcgct gggtgcttcc ggcaatcgcc agggggcgct gtatccgtta
    54001 ttaagcaaac acgatgaggc gctaaaccgc tttttctcta atgcgtttac ttttgctcgt
    54061 cggttttacg accaattacc cgttaaattt gatcatgact ggtgcggcgt cacgcagtta
    54121 ggctgggatg agaaaagcca gcataaaatc gcacagatgt tgtcaatgga tttacccgca
    54181 gaactggctg tagccgttga ggcaaatgcg gttgaacaaa ttacgggcgt tgcgacaaat
    54241 tgcagcggca ttacttatcc gcaaggtggt tggctgtgcc cagcagaact gacccgtaat
    54301 gtgctggaac tggcgcaaca gcagggtttg cagatttatt atcaatatca gttacagaat
    54361 ttatcccgta aggatgactg ttggttgttg aattttgcag gagatcagca agcaacacac
    54421 agcgtagtgg tactggcgaa cgggcatcaa atcagccgtt tcagccaaac gtcgactctc
    54481 ccggtgtatt cggttgccgg gcaggtcagc catattccga caacgccgga actggcagag
    54541 ctgaagcagg tgctgtgcta tgacggttat ctcacgccac aaaatccggc gaatcaacat
    54601 cattgtattg gtgccagtta tcatcgcggc agcgaagata cggcgtacag tgaggacgat
    54661 cagcagcaga atcgccagcg gttgattgat tgtttcccgc aggcacagtg ggcaaaagag
    54721 gttgatgtca gtgataaaga ggcgcgctgc ggtgtgcgtt gtgccacccg cgatcatctg
    54781 ccaatggtag gcaatgttcc cgattatgag gcaacactcg tggaatatgc gtcgttggcg
    54841 gagcagaaag atgaggcggt aagcgcgccg gtttttgacg atctctttat gtttgcggct
    54901 ttaggttctc gcggtttgtg ttctgccccg ctgtgtgccg agattctggc ggcgcagatg
    54961 agcgacgaac cgattccgat ggatgccagt acgctgacgg cgttaaaccc gaatcggtta
    55021 tgggtgcgga aattgttgaa gggtaaagcg gttaaggcgg ggtaatctgc tctcgcagtg
    55081 tttgccggat gcggcgtgaa cgccttatcc gacctacgtg tgacgtgtag gcctgataag
    55141 acgcgataag cgtcgcatca ggcatggtgc tccagacgcc gcaacttact gctgtgacgc
    55201 ctgctgaaac aggttttccc acatatcggt caccagcgcc tggtcacgcg gcgacaattc
    55261 accggcacca atggcttttt ccagactctg gctaacggtc gtatgcaccg cctgagcgga
    55321 gtggtcatca ccactttcca gttctgcgat ggctaatgtc aggtggccac gcaaataccc
    55381 actggcaaac aactcatcat cactggcatg gtcaaccatg ccgtcgatta atgccagaat
    55441 gcgtgattca aactccgcga tcatcttctt tcctcattgt aaaacgtggc accggcttca
    55501 gttgagcgct tccggccacg ggaactgttc cgccgtaagt tccggcgtgt gataataatt
    55561 ttgtagtgct ttaatgaagc gtgccggacg ctcgggaatg ccatttgtca gataatccat
    55621 cacctgcgca tgtacccgcc gttgaaacac aacacgatcc ggttcgaagt cgccttccag
    55681 attgtcgcaa ctgacgttaa acggatatcc cgccgccaca cagaacaacc agtcgagtgc
    55741 ctgcggcttc acttcaacat cttcaaactg gctttgcgtt tgggcatcac gcccatccgg
    55801 gcaataccag tagccgaaat caaccagctc acggcgcgct tttccggcaa tacaccagtg
    55861 cgaaatctca tgaattgcgc tggcataaaa gccgtgggca aagacaattc ggttatacgg
    55921 tacttccgca tcagcaggaa gatagatcgg ttcgtcgtcg cctttaatca gacgggtatt
    55981 aaattcatcg gcaaagcagc tattaaaaat ttcaatcaac tgctcatagt ggtgtgtact
    56041 gttcattagt tcatccccaa ccagtggagg atctcctgtc cgtggctatc gtaaagtaat
    56101 ttggcactca tcaccgccga gacgataaca atcatcgggc ggatcagctt ttgtcctttg
    56161 ctcaacacca gccgtgaacc catgcgcgcg ccgaggaact gccccaccag catgacaaag
    56221 cccgtcgccc aaatcacttt gccgccgaga ataaacagca gcagaccgcc aatgtttgac
    56281 gttgcgttga gtaatttggc gtgagccgtg gctttggcga ggttgaatcc gcacagcgta
    56341 acgaaggcca gcgcgtaaaa cgatccggca gccgggccaa agaatccgtc ataaaaaccg
    56401 acgcagccac cggcaattaa cgcgaagggt aaaccgtaca tccggcgctg gcgatcttct
    56461 tcacccagct ttggcatcag caagaaatag aggccgatac aaatcaccag aatgggcaaa
    56521 atctgccgta agacatcagc ctgaacgtac tgtaccaaca gtgcgccgct cattgagccg
    56581 acaaaggtca tggcaatatt gagtttctga tcgcttaaac taaccacttt gcggcgaata
    56641 aagtagatag tggcggaaat agagccgccg caggcttgca gtttattggt tgccagcgcg
    56701 ttagcgggag acattcccgc agccatcaat gccggaatgg tgagtaaccc accgccaccg
    56761 gcaatcgagt cgataaatcc cgccagcatg gcgacaaaaa agagaactcc cagcaacagc
    56821 ggggaaacca taaacaggct attaaacgtt tccattagat cacgtgctca tccagtagcg
    56881 cctggcagga aggcggcaac ggaggcggtg tcttcttctc aggctttgtt gttcccggtt
    56941 ttagaggtgc aaaccagctt tgcagttctg ccccgcaacc atcgcctgat ggcggtaaag
    57001 gttgatcttc acactccaga ctatcggcag ggcaacgtaa tcgtacatgc atatgcgcgc
    57061 gatgctggaa ccagggtcgc actttgcgca accagtcgcg atcggtgccc gcatcaaggc
    57121 aaagttgttg tttaatcgcc ggattaacaa aaatgcgcgt gacgtctttg tcctgggcag
    57181 cgagtttgat caagctgaaa atttctggct tccacagcgt ggagacaacg tgtttaccgt
    57241 cgcgggaaac taagtccagt gcttgcgggc gcaagagctg cgcggaggtc cagcgagttt
    57301 tcggcagttg cagaaagata tcgacatcca gtccggtctg gtggctggca tgaccgccgt
    57361 tgaaacgccc accagcgggc atccccatat cgccaatcag caccgtaccc atgcccagat
    57421 tgctcacctg gctactcaga cgctggataa acatcaccag atccgggtga ccgaaatagc
    57481 gacgctgatc ggtacgcatg acctgataat gttcggactg tatcggcaac gtgtcagcgc
    57541 cgacaataca gccattagaa aaactgccta tcgattgtgc gctacccggc acaggttggg
    57601 ttattttttg ccacggcgtc gctgccaggc tagcgctact ggcaagcaga gccagcagcg
    57661 caatcgcggt tttgttcatt ttttaccagc gtggaatatc agtcttcaca tcggcatttt
    57721 gcgcccgctg ccgtaacagg tgatccatta aaacgatcgc cagcatcgct tcagcgatcg
    57781 gcactgcacg gatcccgaca cagggatcgt gacggccttt ggtgatcatc tcaacttctt
    57841 cgccaaagcg gttaatagtg cgccccggca cggtaatgct ggaggtcggt ttcagcgcca
    57901 tatgagcaat gatttgctgc ccgctgctga taccgccgag aatgccgccc gcatggttgc
    57961 tctggaaacc gtctttggtg atttcgtcgc ggttctggct gccgcgcagc gccactacgt
    58021 caaaaccgtc gccaatttcc acgcctttca ccgcgttgat gctcatcagc gcatgggcga
    58081 tgtcggcatc caggcggtca aagaccggct cgccaagtcc ggcggggaca ccactggcaa
    58141 caacggtgac tttcgcgccg atggagtcgc cctctttttt cagcgcgcgc atcagttcat
    58201 ctaacgcgtc gattttgtcc gggtccgggc agaagaacgg attttgttca accagcgacc
    58261 agtctttgat ttccagcgga atgtcgccca tctgggtcaa gcagccgcgg atttcaatac
    58321 caaatttctc ggcgagatat tttttggcaa tcgcccctgc cgccacgcgc atggcggttt
    58381 cgcgggcgga agaacgtcca ccgccgcgat aatcgcgcag accgtatttt tgttcgtagg
    58441 tgtaatcagc atggcctggg cggaaaacgt ctttaattgc gctgtaatcc tgagagcgct
    58501 ggtcagtgtt ttcgatcaac aagccaatgc tggtgccagt agtaacgcct tcaaaaacac
    58561 cggagagaat tttgacctga tccggctcgc ggcgctgggt ggtatagcgc gatgtcccag
    58621 ggcgacgacg gtcgaggtca tgttgcaggt ccgcttccgt cagcggaatg cctggcggaa
    58681 caccatcgac gatgcagccg agcgccagcc catgcgattc gccgaaggtg gttacgcgaa
    58741 agagttgtcc aattgtgttt ccagccatca cggctccgtt attgttgtgt ttgcgtgttt
    58801 acttaatctt tataaatcgc gaaatattca cgggccgcca gcagctgctc tttggtgagc
    58861 ataaacacac catcgccgcc gttatcaaac tccagccagg tgaacggaac atccggatat
    58921 tgttccataa gatgtaccat gctgttgccg acttcacaaa tcaacacgcc atcatcagca
    58981 aggtaatctg ccgcgtttcc aagaatgcga cgcgtcagtt taaggccgtc ggtgccagat
    59041 gccaggccca gttccggctc gtgacggtat tcgtttggca ggtcggacat atcttccgcg
    59101 tcgacatacg gcgggttagt gacaatcaga tcgtactgca ctttcggcaa atcacggaac
    59161 agatcggaac gaatcggaat gacgttgtgg atcagaccgt gttcttcgat gttctgttcg
    59221 gcaaccgcca gcgcgtcggg agagatatct accgcgtcga cttccgcttc cgggaaggca
    59281 taagcacagg caatggcgat gcagccgcta ccggtacaca tatctaaaat atgctgcggt
    59341 tgcttgctaa tgagtccggc aaatttattg ttgatcagtt caccaatcgg cgagcgcggc
    59401 accagcacgc gttcatcgac gtaaaattca tgaccgcaga accaggcttt gttggtcagg
    59461 taagccaccg gaatgcgctc gttgacgcgg cggatcacgc gttcaacaat acggtgtttt
    59521 tcgctggagg tcagacgcgc ggtgcgcata tcttccggga tatccagcgg caggtagagc
    59581 gatggtaaca ccagctgtac ggcttcatcc cacgggttat cggtgccgtg accgtaccag
    59641 atatttgccg cgctgaagcg gcttaccgac cagcgcaaca tgtcctgaat ggtttgcagc
    59701 tcatttactg cttcatcaac gaaaatttta tccacgtatt cctccagggc atgatcgcaa
    59761 taatttcggc ggctagtttg ccatgaagat gacgataaat cagcattcac gcgcggtgag
    59821 tgaggaaaaa tacgtttaaa acgatcgatt gtgctacgag tcgggtaaac tgtaggaaaa
    59881 ttagaaatag agacagataa atgaaaaaga aaacaacact tagcgaggag gaccaggctc
    59941 tgtttcgcca gttgatggcg gggactcgca agattaagca ggacacgatt gtccaccgac
    60001 cgcagcgtaa aaaaatcagc gaagtgccgg tgaaacgctt gatccaggag caggctgatg
    60061 ccagccatta tttctccgat gagtttcagc cgttattaaa taccgaaggt ccggtgaaat
    60121 atgttcgccc ggatgtcagc cattttgagg cgaagaaact gcgccgcggc gattattcac
    60181 cggaattgtt tttggattta cacggtctga cgcagctgca ggccaagcag gaactgggcg
    60241 cgttgattgc cgcctgtcgc cgcgaacatg tgttttgtgc ctgcgtgatg catgggcacg
    60301 ggaagcatat tttgaagcaa caaacaccgc tgtggctggc acaacatccg catgtgatgg
    60361 catttcacca ggcaccgaaa gagtacggcg gtgatgcggc tttgttggtg ttgattgaag
    60421 tggaagagtg gttgccgccg gagttgccct gaatgctggc agcaaatgcc ggagcagcgt
    60481 aaacgcctta tccggcctac aaatatgtga taattcaata aattgcaatt cacttcgtag
    60541 gcctgataag acgcatcaga gcaatactac gaactacagt tcattcaata catactgaaa
    60601 acgacacgtc gaatcaatcg gttgcccact actggtattc acattcacca caatatcgtg
    60661 tagtacttta tccgttaccg gatgacgagt tggcgtaacc gttaccgttg atttatcaca
    60721 accgtaagag gtaaaggtta acaattctga taccgcgcgg cagtatgctc ctctgccatc
    60781 attatcagaa aaattactgc catcacagag tctgccgcct tccatcaggt aggtcgaaag
    60841 cgtggtggtc actgttgacc ccgtggactg aattcgtaac gccttattat ggctgacata
    60901 attttcactc caggagatat cgtatgaagt gaccggtatc gttgggcata ttgtgttatg
    60961 cactgtaggg gccgtatagc ttccaaatcc gggtaaatct gtataggaat aaaaggatgc
    61021 gatccaaggg caaccataat catcagatgc aacggtaatt ttaaacgtca tatatgcaca
    61081 acgaaaaatt ttcgtatcta tgccagggca atttgagtta ttaattgtat caaagtcagt
    61141 cagtaccaaa gttggagctg aacccgaccc agaccaggaa ataacccgca acgtatcgcc
    61201 tttatacgca aaagaatcat atttcccata tgtaacctcc tttggcgtta tcaccgtaac
    61261 attgaaaaca ctgctcgcta cagcaccggt taccttgtcc atggcataat aaactctcac
    61321 actgtcaaaa ctcgcactca taagtttatc tccagcataa ctcttagatg cgccgacaac
    61381 actcagaacg agcagcatta ggctcgcgaa aattatggtc cattttttca tttcagaaat
    61441 actcaaaggt tgccgtcact gtggcactaa agtctccgga cgttacatct cgcccgctct
    61501 ttgcctgtaa ccaggtatta aaatttaggc tgttatttcc ctgattcagc gggagcgtga
    61561 cacctgttgt attgttgatt ggcaccgatg tgcctgccgc agtttcaata ccaatcccag
    61621 cgccggatgc tgatgacgag gagtcaaagg ccagaaagcc gggtagagca ctatcttctg
    61681 ttcctgtcaa cgttactttg acattaaaca atgtagaact atgacaatct ttcaattgaa
    61741 acaccaatgg cactcgctct gactgcccaa ggttcattaa atcgctggcg gcaatggcag
    61801 gaaaatgtac ttctgccaac tggctaccat taatcactgg cgtacacgat ttactgatta
    61861 aattgccggt aaagtgcaaa ttatctcctg ctattgtggt tccactgccc agcaacatca
    61921 ggctactaat aaaaaacaac gtacgctttt tcttcattgg taatctacct gtaacgtggc
    61981 ccaggcctca aagtccgcct ctggcagaac gacaccactc ttttttactg gcacagccgt
    62041 taacaccggt aaatcggttt cattcaccat aagtggcgta ttgatcgtaa aactctgccc
    62101 tgcctgtttg agctgaatgc ctaaaccagt gatattgctg cttactgcag cattatcaaa
    62161 atcgcttagt gtgccactcc agctcaaggt catttccatt aatgggtcgc gaaccgccgt
    62221 atcgcaggta atggaatacg gcacggtttg ctcataattg ctgccattaa tgtcatcaat
    62281 aagcacatcc tgaaaagtta tttcaatggt ttgagcgttg ctaatcgtgc agttaggcgg
    62341 ctgcactaaa taaccgtgaa aagagacgtt tgagagtgcc atcgagcaaa agccccacag
    62401 caaaataagc gagatccgtt tcattggtaa tccaccacaa ttgtggcact ggcgttaaat
    62461 tcagctgccg ctaacgttgt accactctgt ttcaccggca cagcttccag aacgggaact
    62521 cccgaggaaa aattaaatgg caaccatgag ccagaggtta agtcgagtat tgtgtgatca
    62581 ctacttttct gaacccgaat accaaaccca ggaatacccg tgctgataac tgtttcaccg
    62641 ttaatcacga ccgttgtcgc ctgcatctgt agacgcaggt catccattgc cagattgtta
    62701 caaacaagcg agtagtcaat gggacgttta taatcaaccc cattgatctt attaatgaat
    62761 acattaccaa actcaactgc cgcaccattt accgtacaag gaggaggggc atcgacaagc
    62821 acccgtaaat tcatacttac tgacttactc gctgcaactg caaagccgct accaacacaa
    62881 catatcagta acccagttac cgcccagaca gaacaagatc ctttcatact ggctctcccg
    62941 gcctcagcct ttgcggctct gctcttcatc gacctggcaa ctgttaccgt tgcaagtgaa
    63001 aaagagcggc atccgagcgc catagtcgtt gacataggtc agtaccggca cgctacccat
    63061 cttcacattc agcggtgcag aggttttcgg cgacatcact attggcgaga aaccggctgc
    63121 aggattgcca tttttctgtg tgctggcatt actgaaaatg acgtaatacg gcgtcgggtt
    63181 attgacggta aattcctgac cgttgcgggt taaagtaacc ttatattgcc aggggttttt
    63241 catgctgacg ttctccagcg ccttcggtcg ccagaacaac ttaatacgcg tttgtagtgc
    63301 tatttgcagc gtgttggctt tgtttgattt tggcgggatc tcacgcacgt tgaagtagaa
    63361 caggctttca cgatcggcag gcaatttgtt gatatccggc attccctgca cttttacctg
    63421 cccgttcatc attgaatcga tgcgttgcac cggtggcaga acagttaacg gtgaactgat
    63481 cttgttaccc ttttcatctt cgatccagct ttgggccaga taaggcagtt tcggatcgtt
    63541 attgcgcaac gtcacgctga ttgatttgtc gctttcatta aatatcaaac gggtacgatc
    63601 tggcgttaca gaagcctggg cgacaaacta gcagcgctaa gcagtaacgc tagcgtcgta
    63661 gcacccaatt tggcagagca aagtaaatca gacatggatc attccttaat cacgttattg
    63721 gttagctgaa acagaaacag ggggattggt gggtgtcacc tgttgcgtct gctcctgaat
    63781 gacaggcttt atgtcatcag gaacaaccgg cgcgacgttg cctttatgct ggcatggcag
    63841 taacaagccg ttaaacagat cggcaggcag cgggtccggc aggttgatat cgcaatgcgc
    63901 aacaccactc cagaacacac tcatgtgttc gccaggtttc accccagcca gataaacatt
    63961 gccgtcatca tcgacaaggc ccactgtctg ctcgttatca ttttttactt ctgcgccaaa
    64021 cggaggatgg ctgccgtctt gtaaacgcag caccgccatc gctttttgac cactgatgac
    64081 ggcaaatttg cggtagccaa tcgccccttc agttagcgtg gcttgtacca ccgactgggt
    64141 tgcttcggcg ttttccggca gtttgttgag gtcgatatac gcctgattgc ggtaatagtt
    64201 attgacgtca gaaacgacgg ctttaccaaa catattggta taaacagcag caccgttacc
    64261 ttctaccgga acatcggcaa cgccatcggc atcaatcaac aagcgtgtcc cgcccatatt
    64321 ctgggtacgg tgaagtgcgc caccgtgggc agtaagcgtt gcgccgccct gtaacgacaa
    64381 gcccgcagag gtgtattgcc cttcatggta gttcgcactg aggtcaacct gcgccagcga
    64441 accatcatgg ctgtaatagc cgtcaacgct ggtgtgtttg tcactggtgc cgacgttcaa
    64501 ctgatagtga gtcgcgtcat cgacacggct gaaataaccg acctgactgc tgtccgtccc
    64561 actgccatag ttaccgttat agctaacggt actgttgtca ccccacggca tactgagcga
    64621 aatgtacatg cctttgtcgg cctggttgtc atactcgtaa cggtagccag tcatcgagat
    64681 gctgacatta cgaatactgc ccatattgaa atagtgcgag agcatgatgt tgtagtttgt
    64741 ctgctcctcg cgatcccagt aggtatggcg ggtatagttg agataaaccg aaacacccgc
    64801 atcgcggaag ttctggttat aagtggcagt gtacatctct ttgtcgttgc ccgtgcgtac
    64861 cattccgctg tcactggcat ccagatactc gctcatggtc ataaagttct cttccgagaa
    64921 gcgatatcca gcaaaagtaa cgcggctgtt gagctggtca aagtctttgg aataactcac
    64981 acggaaggag ttaccgtcca gcgaaccttt gccataagcg gtgtctttat ccagtttggt
    65041 gtgcgagtga gtaacatcaa acgcgaccgc gccgaatgta gacaaatcgc gaccgacgcc
    65101 aagcgccgca gactgatagt tttcatctcc cagtgcgccg ccatataacg accagccgtt
    65161 agcgatcccc caggaagctt cagcaccaga aaaaaattca ccctcgacat ggtgtcccca
    65221 ctcttgcgga cggcccatca tgatcttata gcgaacctga cctggacgag tgaggtatgg
    65281 catcaaggcg gtgctgatgt catattcctg cacctggccg ttctgttctt caatgcgaat
    65341 atgcaacgta ccggagacag aatcaccaag atcctgaata cgaaacggtc cagccggaac
    65401 ctgcgtttcg taaatcacac gccccatctg gctgacggtc acttttgctg tggtgtgtgc
    65461 cacgccggaa atgtctggcg cgtagccgcg cagattggga ggcaacattt gatcgtcagt
    65521 actgacactg ccaccaacat agttaaaacc atcaaaaata tcggatctga ggtaatcctc
    65581 gcccagcgcc agtttggctt ttaatgatgg taacgcccgc taggcatagt agcgactcca
    65641 ctcccatttt ttttgagttt catcgccgct gaattcatcg tcatcattac tgcgagtatg
    65701 ttgatagtta gtctgccagt cagcacgcat acgccacggc cccaggttaa ccccgaccgt
    65761 cccgttgccg ctgatctcgt tactatcatc accgccattt tcttcgtgcc gggtttgtgc
    65821 gttgatgctg tagtccgcga cgatcccgga gatgccgtca tcccaacgtg aaggcggatc
    65881 ccagtcggga taagtatatt cgaggtaagc ctgtggcagt gaaatgacta atgcggactg
    65941 gcttaaatca gccttaattt ccatgccttc cagttgaccg gatttcaggc atttagcatc
    66001 gtggctccat tgcagatttt tcgccacgtc ttcttttaaa ccaaactgcg ctaccagttc
    66061 cggtgtcaga caagcatagc ttttgctcgc gtcatcttca ccagcatacc agtaaatatc
    66121 gtactcttcc gccaatggct gtttatttag ttgaacctgt aaattgtatt ttccgggctc
    66181 aacatatccc tggctggaaa aacgcttcag atcaattttt gtgtcgcctt ttaattccag
    66241 aaaacgggaa tcaaactgaa tgtcgtcttc agcccagaca ctgctataac tccctgacat
    66301 tgccagcgca atgcaccacg gaagaatccg taatcgaaaa agagaatggt caggcataca
    66361 cgtttctatc cgtttttatt cttgttcggt caaaatccat ttaaatcccc catagcagtg
    66421 tcgctatggg ggtatgcctt acaggtaagt aatctggaag gtggtatttg cttcgaaatt
    66481 acccagatct ggcgcatcag ctgcgccgac cagccaggct ttaaagttca gtgtctgctt
    66541 cgctttgcct ttattggtag aagtgtcttt cactattttc tggtcaatac ccatgccgct
    66601 tttgtaagag gtacccagag agtcaccgat cgccaggcta acattgttaa atgccgcaca
    66661 ggtatcggta ttgaaaatgg tgtaataatt gccgctattt gcagaagaaa cggtaccagt
    66721 aaaggtagtg gtcattgttt cctgagtatc aaatacgcaa tcttgcagac gaatctggaa
    66781 acttttcggc gtggtggtgc cgttattttt cagtttttcg gcaccgatat cacccatatc
    66841 aacttccagt ttgtgatcgt ccggaacgat agaacacact gagtcttcaa tggtgccgta
    66901 aaaacgtgca gtaccgttgt tacccgcagc gattgttgcc gtagaggtga acacgcccat
    66961 caccattgcc gcagcaatag ctgttttaac aaacttactc att
//
LOCUS       DAZPDU010000003        64592 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZPDU010000003 DAZPDU010000000
VERSION     DAZPDU010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 64592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64592
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.003"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(36..1334)
                     /gene="eno"
                     /locus_tag="ACHURS_000145"
     CDS             complement(36..1334)
                     /gene="eno"
                     /locus_tag="ACHURS_000145"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFR1235983.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(1422..3059)
                     /gene="pyrG"
                     /locus_tag="ACHURS_000146"
     CDS             complement(1422..3059)
                     /gene="pyrG"
                     /locus_tag="ACHURS_000146"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFR1235984.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(3287..4078)
                     /gene="mazG"
                     /locus_tag="ACHURS_000147"
     CDS             complement(3287..4078)
                     /gene="mazG"
                     /locus_tag="ACHURS_000147"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFR1235985.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4149..4484)
                     /gene="mazF"
                     /locus_tag="ACHURS_000148"
     CDS             complement(4149..4484)
                     /gene="mazF"
                     /locus_tag="ACHURS_000148"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFR1235986.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(4484..4732)
                     /gene="mazE"
                     /locus_tag="ACHURS_000149"
     CDS             complement(4484..4732)
                     /gene="mazE"
                     /locus_tag="ACHURS_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFR1235987.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(4810..7044)
                     /gene="relA"
                     /locus_tag="ACHURS_000150"
     CDS             complement(4810..7044)
                     /gene="relA"
                     /locus_tag="ACHURS_000150"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFR1235988.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7092..8393)
                     /gene="rlmD"
                     /locus_tag="ACHURS_000151"
     CDS             complement(7092..8393)
                     /gene="rlmD"
                     /locus_tag="ACHURS_000151"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFR1235989.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPGRETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVMACLGSLQVMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8450..11206
                     /gene="barA"
                     /locus_tag="ACHURS_000152"
     CDS             8450..11206
                     /gene="barA"
                     /locus_tag="ACHURS_000152"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFR1235990.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11438..12778)
                     /gene="gudD"
                     /locus_tag="ACHURS_000153"
     CDS             complement(11438..12778)
                     /gene="gudD"
                     /locus_tag="ACHURS_000153"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFR1235991.1"
                     /translation="MSSQFTTSVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPYGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12799..14139)
                     /locus_tag="ACHURS_000154"
     CDS             complement(12799..14139)
                     /locus_tag="ACHURS_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFR1235992.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14141..15493)
                     /gene="gudP"
                     /locus_tag="ACHURS_000155"
     CDS             complement(14141..15493)
                     /gene="gudP"
                     /locus_tag="ACHURS_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFR1235993.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(15928..16377)
                     /locus_tag="ACHURS_000156"
     CDS             complement(15928..16377)
                     /locus_tag="ACHURS_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFR1235994.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16395..17177)
                     /gene="truC"
                     /locus_tag="ACHURS_000157"
     CDS             complement(16395..17177)
                     /gene="truC"
                     /locus_tag="ACHURS_000157"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFR1235995.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17177..17506)
                     /locus_tag="ACHURS_000158"
     CDS             complement(17177..17506)
                     /locus_tag="ACHURS_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFR1235996.1"
                     /translation="MTTHDRVRFQLQTLEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18128..18673)
                     /gene="syd"
                     /locus_tag="ACHURS_000159"
     CDS             complement(18128..18673)
                     /gene="syd"
                     /locus_tag="ACHURS_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFR1235997.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18741..19589
                     /gene="queF"
                     /locus_tag="ACHURS_000160"
     CDS             18741..19589
                     /gene="queF"
                     /locus_tag="ACHURS_000160"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417274.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFR1235998.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19701..21065
                     /gene="ppnN"
                     /locus_tag="ACHURS_000161"
     CDS             19701..21065
                     /gene="ppnN"
                     /locus_tag="ACHURS_000161"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFR1235999.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLFLTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            21621..22910
                     /gene="sdaC"
                     /locus_tag="ACHURS_000162"
     CDS             21621..22910
                     /gene="sdaC"
                     /locus_tag="ACHURS_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFR1236000.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            22968..24335
                     /gene="sdaB"
                     /locus_tag="ACHURS_000163"
     CDS             22968..24335
                     /gene="sdaB"
                     /locus_tag="ACHURS_000163"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFR1236001.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            24447..25202
                     /gene="xni"
                     /locus_tag="ACHURS_000164"
     CDS             24447..25202
                     /gene="xni"
                     /locus_tag="ACHURS_000164"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFR1236002.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(25307..26455)
                     /gene="fucO"
                     /locus_tag="ACHURS_000165"
     CDS             complement(25307..26455)
                     /gene="fucO"
                     /locus_tag="ACHURS_000165"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFR1236003.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(26483..27130)
                     /gene="fucA"
                     /locus_tag="ACHURS_000166"
     CDS             complement(26483..27130)
                     /gene="fucA"
                     /locus_tag="ACHURS_000166"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFR1236004.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYVTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            27677..28993
                     /gene="fucP"
                     /locus_tag="ACHURS_000167"
     CDS             27677..28993
                     /gene="fucP"
                     /locus_tag="ACHURS_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFR1236005.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEILGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPTLCFAVIFIFARFRSQTATN"
     gene            29026..30801
                     /gene="fucI"
                     /locus_tag="ACHURS_000168"
     CDS             29026..30801
                     /gene="fucI"
                     /locus_tag="ACHURS_000168"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFR1236006.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALTWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGSVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            30910..32328
                     /gene="fucK"
                     /locus_tag="ACHURS_000169"
     CDS             30910..32328
                     /gene="fucK"
                     /locus_tag="ACHURS_000169"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFR1236007.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYADSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            32330..32752
                     /gene="fucU"
                     /locus_tag="ACHURS_000170"
     CDS             32330..32752
                     /gene="fucU"
                     /locus_tag="ACHURS_000170"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFR1236008.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            32810..33541
                     /gene="fucR"
                     /locus_tag="ACHURS_000171"
     CDS             32810..33541
                     /gene="fucR"
                     /locus_tag="ACHURS_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFR1236009.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(33585..34685)
                     /gene="rlmM"
                     /locus_tag="ACHURS_000172"
     CDS             complement(33585..34685)
                     /gene="rlmM"
                     /locus_tag="ACHURS_000172"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFR1236010.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVDMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(34678..35073)
                     /locus_tag="ACHURS_000173"
     CDS             complement(34678..35073)
                     /locus_tag="ACHURS_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFR1236011.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKCKGVSHE"
     gene            complement(35092..36009)
                     /gene="gcvA"
                     /locus_tag="ACHURS_000174"
     CDS             complement(35092..36009)
                     /gene="gcvA"
                     /locus_tag="ACHURS_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFR1236012.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(36360..36587)
                     /locus_tag="ACHURS_000175"
     CDS             complement(36360..36587)
                     /locus_tag="ACHURS_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFR1236013.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            36779..37984
                     /gene="csdA"
                     /locus_tag="ACHURS_000176"
     CDS             36779..37984
                     /gene="csdA"
                     /locus_tag="ACHURS_000176"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFR1236014.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            37984..38427
                     /gene="csdE"
                     /locus_tag="ACHURS_000177"
     CDS             37984..38427
                     /gene="csdE"
                     /locus_tag="ACHURS_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFR1236015.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVTENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(38478..39284)
                     /gene="tcdA"
                     /locus_tag="ACHURS_000178"
     CDS             complement(38478..39284)
                     /gene="tcdA"
                     /locus_tag="ACHURS_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFR1236016.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(39361..40458)
                     /gene="mltA"
                     /locus_tag="ACHURS_000179"
     CDS             complement(39361..40458)
                     /gene="mltA"
                     /locus_tag="ACHURS_000179"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFR1236017.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            40667..40743
                     /locus_tag="ACHURS_000180"
     tRNA            40667..40743
                     /locus_tag="ACHURS_000180"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40701..40703,aa:Met,seq:cat)
     gene            complement(40818..42071)
                     /gene="amiC"
                     /locus_tag="ACHURS_000181"
     CDS             complement(40818..42071)
                     /gene="amiC"
                     /locus_tag="ACHURS_000181"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFR1236018.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            42303..43634
                     /gene="argA"
                     /locus_tag="ACHURS_000182"
     CDS             42303..43634
                     /gene="argA"
                     /locus_tag="ACHURS_000182"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFR1236019.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IRRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQLDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(43696..45522)
                     /gene="recD"
                     /locus_tag="ACHURS_000183"
     CDS             complement(43696..45522)
                     /gene="recD"
                     /locus_tag="ACHURS_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFR1236020.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHSHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPIVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRG"
     gene            complement(45522..49064)
                     /gene="recB"
                     /locus_tag="ACHURS_000184"
     CDS             complement(45522..49064)
                     /gene="recB"
                     /locus_tag="ACHURS_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFR1236021.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYEHLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(49057..51945)
                     /gene="ptrA"
                     /locus_tag="ACHURS_000185"
     CDS             complement(49057..51945)
                     /gene="ptrA"
                     /locus_tag="ACHURS_000185"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFR1236022.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPAMPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYPQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(52121..55489)
                     /gene="recC"
                     /locus_tag="ACHURS_000186"
     CDS             complement(52121..55489)
                     /gene="recC"
                     /locus_tag="ACHURS_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFR1236023.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFSIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHQLGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIRAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLSAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(55502..55825)
                     /locus_tag="ACHURS_000187"
     CDS             complement(55502..55825)
                     /locus_tag="ACHURS_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFR1236024.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(55810..56217)
                     /locus_tag="ACHURS_000188"
     CDS             complement(55810..56217)
                     /locus_tag="ACHURS_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFR1236025.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSIESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDARVCLRLLADNEALLVAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(56214..56777)
                     /locus_tag="ACHURS_000189"
     CDS             complement(56214..56777)
                     /locus_tag="ACHURS_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFR1236026.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(56768..57238)
                     /locus_tag="ACHURS_000190"
                     /pseudo
     CDS             complement(56768..57238)
                     /locus_tag="ACHURS_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(57423..58217)
                     /gene="thyA"
                     /locus_tag="ACHURS_000191"
     CDS             complement(57423..58217)
                     /gene="thyA"
                     /locus_tag="ACHURS_000191"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFR1236027.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(58224..59099)
                     /gene="lgt"
                     /locus_tag="ACHURS_000192"
     CDS             complement(58224..59099)
                     /gene="lgt"
                     /locus_tag="ACHURS_000192"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFR1236028.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(59250..61496)
                     /gene="ptsP"
                     /locus_tag="ACHURS_000193"
     CDS             complement(59250..61496)
                     /gene="ptsP"
                     /locus_tag="ACHURS_000193"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFR1236029.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(61509..62039)
                     /gene="rppH"
                     /locus_tag="ACHURS_000194"
     CDS             complement(61509..62039)
                     /gene="rppH"
                     /locus_tag="ACHURS_000194"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFR1236030.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNTSAYRRKRG"
     gene            complement(62392..62538)
                     /gene="ygdT"
                     /locus_tag="ACHURS_000195"
     CDS             complement(62392..62538)
                     /gene="ygdT"
                     /locus_tag="ACHURS_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFR1236031.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            62724..63413
                     /gene="mutH"
                     /locus_tag="ACHURS_000196"
     CDS             62724..63413
                     /gene="mutH"
                     /locus_tag="ACHURS_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFR1236032.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            63482..64195
                     /locus_tag="ACHURS_000197"
     CDS             63482..64195
                     /locus_tag="ACHURS_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFR1236033.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            64333..64551
                     /gene="ygdR"
                     /locus_tag="ACHURS_000198"
     CDS             64333..64551
                     /gene="ygdR"
                     /locus_tag="ACHURS_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFR1236034.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
BASE COUNT        15653 a        17368 c        15826 g        15745 t
ORIGIN      
        1 ggaggctggc atttttaaat cagataaagt cagtcttatg cctggccttt gatctcttta
       61 cgaccgttgt acggtgcttt ttcgcccaga gcttcttcga tacgaatcag ctggttgtat
      121 ttagcaacac ggtcagaacg gctcatagaa ccagttttga tctggcctgc agcagtacca
      181 acagccaggt cagcgatggt agcgtcttca gtttcgccag aacggtgaga gataactgca
      241 gtgtagccag catctttcgc catcttgatt gcagccagag tttcggtcag agaaccgatc
      301 tggttgaatt tgatcaggat ggagttagcg ataccttttt cgataccttc tttcaggatc
      361 ttggtgttgg ttacgaacag gtcgtcacca accagctgga ttttgtcgcc cagaactttg
      421 gtctggtatg cgaaaccgtc ccagtcagat tcgtccagac cgtcttcgat agatacgatc
      481 ggatactgtt tggtcagttc ttccaggaag tgagtgaatt cttcagaggt gaaagctttg
      541 ttgccttcgc cagccagaac gtatttaccg tctttgtaga attcagaagc tgcgcagtcc
      601 atcgccagag tgatgtcttt gcccagttca taaccagcag ctttaacagc ttcagcgata
      661 acagccagag cttcagcgtt ggaacccagg ttcggcgcat agccaccttc gtcaccaact
      721 gcagtgttca tgcctttcgc tttcagaact tttgccaggt gatggaaaac ttcagaaccc
      781 atgcggatgg cttctttcac agttttcgcg ccaaccggct gaatcatgaa ttcctggata
      841 tcaacgttgt tgtcagcgtg ctcaccaccg ttgatgatgt tcatcatcgg aaccggcatg
      901 gagtatttgc ccggagtgcc gttcagttca gcgatgtgct cgtacagcgg catcccttta
      961 gcggcagcag cagctttggc gttagccaga gatacagcca ggattgcgtt cgcgccgaat
     1021 ttggatttgt tttcggtgcc gtccaggtcg atcatgatct tgtcaatgcc agcctgatct
     1081 ttagcgtctt tgccaatcag tgcctgagcg atcgggccgt ttaccgcagc aacagctttg
     1141 gttacgcctt tacccaggaa acgggatttg tcgccatcgc gcagttccag agcttcacgg
     1201 gaaccagtag aagcacctga cggagcagct gccataccga cgaaaccacc ctccagatgt
     1261 acttcggctt caacagtcgg gttaccacgg gagtcgatga tttcacgacc gatgattttt
     1321 acgattttgg acattaggtt ttcctcaagt cactagttaa actgaaactc cagacaaaca
     1381 acgcgtaccc aaggtacgcg ttgccgttct aactttttta cttacttcgc ctgacgtttc
     1441 tggaactcgc tggcagcttt cacaaagcct gcaaacagcg ggtgaccatc acgtggagta
     1501 gaagtaaact ccggatggaa ctggcaagcc acgaaccacg ggtgattcgg aacttcgatg
     1561 atctcgacca actgatcatc cccggaacgg cccgcaacgc gcagacctgc atcttcaatc
     1621 tgtttcaaca gcatgttgtt gacttcgtaa cggtgacgat gacgctcaac aattgtcggc
     1681 gcattgtaca gctggcgaac caggctatcg tcaaccaact ggcactgctg tgcgccgaga
     1741 cgcatggtac cgccgagatc gctcttctcg ctacgaactt caacgttgcc gttttcatcg
     1801 cgccactcgg taatcagcgc cacaaccggg tacttacagt ctggcacaaa ttccgtagag
     1861 ttggcgttct ccatgttggc aacatggcga gcgtaatcaa ttaacgccac ctgcataccc
     1921 aggcaaatgc ccagataagg aatattgttc tcacgcgcaa aacgcgcggt cgtaatcatg
     1981 ccttctacgc cacgatagcc gaaaccgcca ggtacgagga ttgcgtccag acctttaagg
     2041 atttcaacgc cgcgcgtttc aacatcttgt gaatcgatca gtttgatgtt gacgctgaca
     2101 cgattcttca gcccaccgtg tttcagtgct tcgatcactg atttataagc atccggcagt
     2161 tcaatgtact tgccgaccat accgatggtg acttcactta ccggattcgc ttcttcgaag
     2221 ataacctgtt cccattcgga cagatttgct tccgggcagt ttaagctgaa tcgtttacaa
     2281 atataatcgt ccagcccctg agatttcaac aggcccggaa ttttatagat ggaatcgacg
     2341 tctttcagag aaataaccgc tttttccgga acattacaga acaatgcaat cttcgcacgt
     2401 tcgttcgccg gaacagcgcg atctgaacga caaatcagga tgtcaggctg gataccgatg
     2461 gagagcagct cttttacaga gtgctgagtc ggtttggttt tgacttcacc agacgctgcc
     2521 atgtacggca ccagcgtcag gtgcataaac agagtgtgct cacggccaat ttcaacagcc
     2581 atctggcgaa tcgcttcgag gaacggcaag gattcgatat cacctactgt accgccgatt
     2641 tctaccagta ctacgtcatg accttcgcca ccttccagca cgcgctcttt gattgcgtta
     2701 gtgatgtgcg gaataacctg cacggttgcg ccgaggtagt caccgcggcg ttctttacgc
     2761 agaacgtcag agtagatacg acccgtggtg aagttgttgc ggcggctcat tttggtacga
     2821 atgaaacgct cgtagtgccc caggtccagg tcggtttcag cgccgtcttc agtaacgaac
     2881 acttccccgt gttggattgg gctcatagta cctggatcga cgttgatgta cggatccagt
     2941 ttcatgatgg tcacattgag gccacgggct tcaagaatgg ctgcgaggga ggctgcggca
     3001 atgcctttac ccagagagga tacgaccccg ccggtcacaa aaatatagtt cgttgtcatg
     3061 ctgaacctga gaagttaggt tgaaaagacg atggaataac caggacggga aagcagtata
     3121 cccgaacatg acctgtgcca caaactttca ttatccctcc tcttcgccag cgcactattg
     3181 aaatcaggag tgagaaaata gcccctttgg ggtaaatgtt tttgacgcaa atcaagcgct
     3241 tgtcatttaa aaaatgacac aaatggcgct tgaccgcgta attcccttag agatcaattt
     3301 cctgccgttt tacctgttgc cagacttctt ccattgtttc gaggtcaaca cctgtcattt
     3361 ccagtccacg cgcggcaaca atacgctcca cttcgcgaaa acgacgctcg aatttttcgt
     3421 tcgctttttg caatgcgatt tctgctttcg tccctaaatg gcgagccaga ttaaccgtgg
     3481 caaacagcag gtcccccatt tcctcctcca gtttagcctg gtcgacaaca gcctgccgcg
     3541 cctcgtacat cacctcgtcg atctcttcgt agactttatc aaccaccggg ccaagcgtcg
     3601 tccagtcgaa gccaacgttg gcgcaacgtt tctggatttt ttgcgcccgc attaaagccg
     3661 gtaaactacg aggaatatcg tccagcgccg aatgctgcgc tttctgcgcg cgctcttcgg
     3721 ttttgatttg ctcccaacgg gcaagcactt cactactgtt ttcggcagaa ctatcagcaa
     3781 aaacatgcgg atggcgacgc tctaatttat cgctaatagc agcgcaaata tcattaaagt
     3841 caaagcgccc ttcttcctga gccatttgcg cgtaaaacac cacctggaat agcagatcgc
     3901 ccagttcacc gcgaagatcg tcaaaatctt cacgggcgat ggcgtccagc acttcgtagg
     3961 tttcttcaag ggtgtaaggc gcaatggtgg caaatgtctg ctctttatcc cacgggcagc
     4021 cgttttccgg atcgcgcagg cgctgcataa tagtgagcaa acggtcgatt tgattcattg
     4081 aattgtcctg aaaattgcgg gtctgtcagg tggaaacctg tgaccagaat agaagtgagt
     4141 tagtaacact acccaatcag tacgttaatt ttggctttaa tgagttgtaa ttcctctggg
     4201 gcaactgttc ctttcttcgt tgctcctctt gcccgccagg cgatactttt tacctgatca
     4261 gctaacgcta cgccatcacg ttcctgaccg gataaaacaa cttcgaacgg atatcctttt
     4321 gattgcgttg tacaaggaac acacagacac atacctgttt tgttgttgta catgaaagga
     4381 ctcaggacaa cagctggacg atgtccagct tgctcgctac cttttgtcgg gtcaaaatca
     4441 acccaaatca gatcgcccat atcgggtacg tatcggctta ccattaccag acttccttat
     4501 ctttcggctc tccccagtcg atattctcgt ggaggttttc cggcgtgatg tcgttgacca
     4561 gttcagcaag cgtaaatacg ggctctttac gcactggctc aataattaat ttgccatcca
     4621 ccaggtcaat cttcacttca tcatcaatat tcagattgag cgcctgcatt aacgtagccg
     4681 ggatccgcac cgccggtgaa tttccccaac gctttacgct actgtggatc ataacccttt
     4741 cctcaaaccg ctatcatatg tagatacagt atatatcaat ctacattgta gatacgagca
     4801 aatttctgcc taactcccgt gcaaccgacg cgcgtcgata acatccggca cctggttgag
     4861 tttacccagc acgcggccca gcacctgcag gttgtaaatc tcaatggtca tgtcgatggt
     4921 tgccagttgc tgttttgtgt cgctacggct ggcaacgcca agcacgttca ccttctcgtt
     4981 ggcgagaatg gtcgtgatat cacgtaacaa cccactacga tcattagcca ccacgcggac
     5041 caccagcgaa tatccggcgg agtagctctc accccatacc gcgtcaacaa tgcgttctgg
     5101 cgcatgagag cgcagttccg ccagttgttc gcaatcggcg cggtgtactg aaataccgcg
     5161 cccctgggta atgaagccga caatctcatc tccaggaatc ggctggcagc agcgcgcgat
     5221 gtggtgcatc aggttgccaa caccttcgac taccacgcga ccgttatctt tactgcggtt
     5281 ttgcggcgtg tagctttttt gctgaagttg cttcagcgcg gcggcgtcct gctcttcggc
     5341 actcggctta ttaaattgcg attgcaggaa gttcaccatc tgattgagac ggatatcccc
     5401 gccaccaatc gccgccagca actcgtcgac atcattaaag ttgtaacgcg gcagcagatg
     5461 tttttctgct tctttcaggc tgatccccag atgttccagc tcgtcgtcaa ggatttgccg
     5521 cccagccaga atgtttttgt cacggtcctg tttacggaac caagcgtgaa ttttcgaacg
     5581 cccacggctg gttgtgacgt aaccgaggtt tgggtttaac cagtcacggc tggggttcgg
     5641 ctgtttctgg gtgataattt caatctggtc gcccatctgc agctggtagg tgaacggcac
     5701 aatgcgcccg ccaatttttg ccccgatgca gcggtgtccg acatcactgt ggatgtggta
     5761 agcgaagtcc agcggcgttg atcccgcagg caaatcaacg acatcacctt tcggcgtaaa
     5821 gacatacacc cggtcgtcaa agacctggct gcgtacttcg tcgagcattt cgccggaatc
     5881 agccatctct tcctgccacg caatcagttt acgcagccag gcaatccggt cttcatgtcc
     5941 cgaacgtgcg ccgccagctg ccgcgccctc tttatatttc cagtgcgcag caacacccaa
     6001 ctctgcatct tcatgcatct gtttggtgcg gatttggatc tcaacggttt tgccacctgg
     6061 ccccagaacc acggtatgaa tagactgata accgtttggt ttcgggttag cgacataatc
     6121 gtcaaactca tccggcaggt ggcgatagtg agtgtgcact atccccagtg cggcatagca
     6181 atcctgtaaa cgctcggcga caatacgtac cgcacgcaca tcaaacagct catcaaaggc
     6241 gaggttcttt ttctgcattt tacgccagat gctgtagatg tgtttcggac gaccatacac
     6301 ttccgcttta acgccttcag ctttcatctc agcgcgcaga tgaccaacga actcttcgat
     6361 gtagtgttcg cggtcgagac gccgttcatg cagcagtttg gcaattcgtt tgtattcggt
     6421 tggatggagg taacggaagc agtaatcttc cagttcccat ttcagttgtc cgattccgag
     6481 acggttagcc agcggtgcgt agatgttggt acactctttt gccgccagta cacgttcatc
     6541 ttccggcgca tcttttactt cgcgcagatg agcaatacgc tccgccagtt tgatgactac
     6601 gcagcgaaaa tcatcgacca tcgccaataa catccggcga acgttatcga cctgttcgga
     6661 ggaaacagaa tcagtgtgcg tcgctttcag ctggcggatc gccgccatat cacgcacgcc
     6721 gtgaataagg ttaacaaccg acttaccgac gctctcacgc agcacatctt cgctgactac
     6781 gttggcatca gccagaggga acagcagcgc cgcccgcagc gtgtcaatgt ccatacttaa
     6841 tgtcgagagg atctccacca tctcaacacc acgccacaat aacagactgg catccggatg
     6901 cccctgcgtc tgttgcagac aatacgccca agtttcggct aagcactcac acgacttctg
     6961 gctggtaata cccagacttg cgatccattt ttccggatca aattcaccag ccttattgat
     7021 atgtgcactt cttaccgcaa ccatcgtcct ctcctttagg gaccagacct gccgaaatcg
     7081 gcaaatcgca actatttaac gcgcgagaaa agtaccatcg attccagatg tcccgtgtgt
     7141 gggaacatat ccagcatcgc cagtcgcgca atggtatatc ctgcttttaa taacgcttcg
     7201 ctatcccgag ccagcgttgc agggttacag gatacataaa ctatacgaat aggttccagt
     7261 tttataattt gctgcataac acctgcggca cctgctcgcg ccgggtccag caacacttta
     7321 tcgaagccgt ttttcgccca cggctgcttt gtgacatctt cttcaagatt ttcgtgataa
     7381 aacgtcacat tctgtaagcc attaagacgc gcattctgct ggcctttttc caccagcgcc
     7441 ggaacgcctt ctacacccac cacactggca gcttgtgtcg ccaatggcag tgtaaagttg
     7501 cccataccgc agaacaaatc cagtacgcga tcttcaggtt gcacatccag ccattccaac
     7561 gcacgcgcta ccattttttg gttcacaccc gcattgacct gaataaaatc gcgcgggcta
     7621 aaagttaagc gcaacccatt tgagtcatac cagggcatct caccagagac ggtttcgagt
     7681 atctcactat cgggggcgag atacagatcc aggccttcag aatgcgaaaa gcgttccagt
     7741 ttttcgcgat ctgccgaact tagcggtgcg gtatggcgca aaatcatcag cgtgccgctg
     7801 gttgcctgta ccagttcaac atgaccaaga tggcgcataa cttgtaagct gcccaggcat
     7861 gccatgactt tgggcagcaa tgcttcaagt tggggcacta aaatggggca ttgtttaacg
     7921 tcgacaatgt cactggagcc cgctttgcga aaccccatct gaagttgctg tgttttcggt
     7981 aagtagttca gacttaaacg cgcgcgacgg cgatagcccc agggaacatc ggcgatcact
     8041 tcagagactt cgtgtttcat taatcgggcg agtgccgcac ttttgcttcg ctgctgtaaa
     8101 tccacgctgg cgtgttgttg ctgacagccg ccgcatacgc caaaatgagg acagcgtggc
     8161 gtttcgcgtc ccgggctatc gcttaaccgg cgtacgactt tagcgcgggc atactgtttt
     8221 ttatcttcag taacagtaac ttccgcgttt tcctgcggca ataatccggg gataaatagc
     8281 gttttgccgt tatgtcgcgc cacgccctga ccaaaagagt cgaggtcgtt gactgaaacg
     8341 gttatgatct gacgcgtcgt cgtgcgtcgt tttgcagagt agaattgcgc cattggcgag
     8401 actttctcaa tttaacagtg tgaccttaat tgtcccataa cggaactcca tgaccaacta
     8461 cagcctgcgc gcacgcatga tgattctgat cctggcaccg accgtcctta ttggtttatt
     8521 gctgagtatc tttttcgttg tgcatcgcta taacgacttg cagcgtcaac tggaagatgc
     8581 cggagccagc attattgagc cgcttgcagt ttctactgaa tatggcatga gcctgcaaaa
     8641 tcgcgaatct atcggtcagt taataagcgt actgcatcgt cgccattccg atattgttcg
     8701 cgcgatttcg gtttatgatg aaaataaccg actctttgtc acctccaatt ttcatcttga
     8761 tccctcatca atgcagctcg gcagcaacgt gccgtttcct cgccagctca ctgtcactcg
     8821 tgacggcgat attatgatcc tccgcacgcc gattatttct gagagttact cccccgacga
     8881 atcgcccagt agcgatgcca aaaatagtca aaatatgttg ggatatattg cgctggagct
     8941 ggatcttaaa tcggttcgct tgcagcaata taaagagatc tttatttcca gcgtgatgat
     9001 gctgttttgt atcggtattg cgcttatttt tggctggcgc ttaatgcgcg atgtaaccgg
     9061 tccgattcgc aacatggtga ataccgtcga ccgcatccgt cgcgggcaac tcgacagccg
     9121 ggtggaagga tttatgctcg gcgagctgga tatgctgaaa aacggtatca actcgatggc
     9181 aatgtcgctg gctgcttatc acgaagagat gcagcacaat atcgaccagg cgacgtccga
     9241 tctgcgtgaa acgctggagc agatggaaat tcagaacgtt gagttagatc tggcgaaaaa
     9301 gcgcgcccag gaagcggcgc gtattaaatc cgagtttctg gcaaatatgt cacacgagct
     9361 gcgtacacca ctgaatggtg ttattggctt tacccgcctg acgctgaaaa cagaattaac
     9421 accaacgcag cgcgatcacc tgaatacgat tgaacgttcg gcaaataatt tgctggcaat
     9481 tattaatgat gttctcgact tctcgaaact ggaagcaggt aagctgattc tggaaagtat
     9541 tccattccca ctacgcagca cgctggatga agtcgttact ctgctggcac attcttctca
     9601 cgataaaggg ttggaactga cgctcaatat taaaagcgac gtgcctgata acgtgatcgg
     9661 cgacccactg cgattacagc aaatcatcac taacctggtg gggaatgcaa ttaaattcac
     9721 cgagaatggc aacattgata ttctggtaga aaaacgtgcg ctgagtaata ccaaagtgca
     9781 gattgaagtg cagattcggg ataccggcat tggtattcct gaacgcgatc aatcgcgctt
     9841 attccaggcc ttccgacagg ctgatgccag tatttcccgc cgtcatggtg gcaccggtct
     9901 ggggctggtg attacacaaa aactggttaa tgaaatgggc ggcgatattt cgttccatag
     9961 ccagccgaat cgcggttcaa ctttctggtt ccacattaat ctcgatctga acccgaacat
    10021 tattatcgaa gggccatcca cccagtgcct cgccggtaag cgcctggcct atgtcgaacc
    10081 aaactccgca gcagcgcaat gcacgctgga tattttaagt gaaacgccgc tggaagtggt
    10141 ttatagccca acgttctccg cgctgcctcc cgcgcattac gacatgatgt tgttaggcat
    10201 cgcggtgacc ttccgcgagc cgctaacaat gcaacatgag cgattagcga aagcggtatc
    10261 gatgaccgat ttcctgatgc tggcacttcc ttgccatgca caagtcaatg cagaaaaact
    10321 caagcaagat ggtatcggcg cgtgtctgct gaaaccatta acacctacgc gcctgttgcc
    10381 tgccctgacg gaattttgtc atcacaaaca aaacacgctt ttgcctgtaa ccgatgaaag
    10441 caagctggca atgacagtca tggcagttga tgacaacccc gctaacctga aacttatcgg
    10501 cgcattgctg gaagatatgg tgcaacatgt ggaactttgc gatagcgggc atcaggcggt
    10561 tgaacgggcg aaacagatgc cgttcgattt gatcttaatg gatattcaaa tgcctgacat
    10621 ggatggcatt cgggcctgcg agctcatcca ccagctcccc catcagcaac aaacgccggt
    10681 tatcgcggta acggcgcatg caatggccgg gcaaaaagag aagctgcttg gcgcagggat
    10741 gagcgattat ctggcgaaac cgattgaaga agagcgattg cataatttgt tgttgcgcta
    10801 caagcctggc agcggtattt cctctcgcgt cgtgacgccc gaagtcaacg aaattgtggt
    10861 gaacccgaat gcgaccctcg actggcaact ggcactacgc caggcagcag gaaaaaccga
    10921 tctagcgcgc gatatgctgc aaatgttact cgatttcctg cctgaagttc gcaacaaagt
    10981 tgaggagcag ctggttggag aaaacccgga aggcctggtt gatttgattc ataaactgca
    11041 tggcagttgc ggctatagcg gtgtgccgcg tatgaagaat ctctgccaac tgatcgaaca
    11101 acagctacgt agcggtacta aagaagaaga tttggagccg gagctgctgg aactgttgga
    11161 cgagatggat aatgtcgcgc gcgaagccag caaaattctc gggtaacgga cgtcttgtcg
    11221 agtagcaatc ggctttttat gacgctggtt tcagattggt gacaaagtgc ctgtgtttat
    11281 gccggatgcg gcgttaacgc cttatccggc ctacaaaacc aattaaattc aatgaattaa
    11341 aaaatgatgt agacctgata catagcacaa caggctattt tgcgtttagc atcagtctca
    11401 agccggctcc agatagagcc ggttttggtt ttctgtctta acgcaccatg cacgggcgct
    11461 tgttatcgaa cgtccagcca ggaatcagat actgcattcc catcgcatcg tcacgcgcgc
    11521 caagcccgtg tttctgatac agctcatggg ctttcatcac ttgatccata tcgatttcta
    11581 cacccagccc cggtttttcc ggcacctgaa ccagaccgcc tttgatctca aacggttctt
    11641 tggtcaagcg ctgattgcct tcctgccaaa tccagtgcgt atcaatcgcg gtgattttac
    11701 ccggtgcagc ggcggcaaca tgggtaaaca tcgccaggga aatatcgaag tggttgtttg
    11761 agtgtgaacc ccaggtcagg ccaaattcat ggcacatttg cgccacacgt accgaacctt
    11821 gcatagtcca gaaatgcgga tccgccagcg ggatatcaac ggattgtaac gagagcgtat
    11881 ggcccatctg ccgccagtcg gtggcgatca tattggttgc agtcggcagg ccagtcgcgc
    11941 gacggaactc tgccatcact tcacgcccgg agaaaccttg ctccgcacca tacggatctt
    12001 ctgcataagc cagcgaacct ttcaggtatt taccaatttt aattgcttcg ttcagcgacc
    12061 aggcaccgtt aggatcgagc gtaatacgcg cctgcgggaa gcgttgcgcc agtgccacaa
    12121 tagactcggc ttcttcttcc ccggccagta caccgccttt cagtttgaaa tcgttgaagc
    12181 catatttttc atacgccgct tccgctaggc gcaccaccgc atccggcgtc atcgcttctt
    12241 catgacgcag gcgataccag tcgcatgagt catccggctg gctttgatac ggcagaggtg
    12301 tggctttgcg attaccgacg aagaacagat aaccgagcat ttcgacttcg ctacgctgtt
    12361 gaccatcgcc cagcagcgat gccacgttta cccccagatg ctgccccagc agatccagca
    12421 ttgccgcttc tatcccggta actacatgaa tagtggtacg caaatcaaat gtctgcaaac
    12481 cgcgcccacc agcatcacga tcggcaaaag tattacgcac cagcgtcaga acgtttttgt
    12541 attcacccag cgttttacct accaccagcg gaatcgcatc ttccagcgtt ttacggattt
    12601 tctcgccgcc gggaatttcc cctacgccag tgtgaccgga attatctttg ataatcacaa
    12661 tattacgcgt aaagaacggt gcgtgtgccc cactcagatt catcagcata ctgtcatgac
    12721 ccgccaccgg gatgacctgc atttcagtaa caacagacgt cgtaaattga gaactcatat
    12781 ttatgtcctt tttcagaatc aatgacggcc gaaaacggga cgtttacggt caaaggtcca
    12841 gccggggatc aggtactgca tcggacctgc gtcgttacgc gcaccgccag gcagacgttt
    12901 ataggcctca tgtgcctttt gtacctgttc ccagtccagt tccacgccca gaccgggcgc
    12961 atcaggaacg gcaatttttc cgtttttaat ctccagcgga tttttggtca ggcgacaatc
    13021 gccctcctgc caaatccagt gggtatcgat agcggtagga ttacccggtg ccgccgcgcc
    13081 cacatgggta aacatcgcca gagagatatc gaaatggtta ttagaatggc agccccaggt
    13141 cagcccccag tcgtcgcaaa gctgcgccac acggactgca ccggaaagcg tccagaagtg
    13201 cggatcggca agtggaatat ctaccgcatt gagcatcacc gcatgaccca tttcgcgcca
    13261 gttggtggcg atcatgttag tcgcgacggg caagccggtc gcccgccgaa actccgccat
    13321 aacttcacga ccggagaaac cctgttctgc gccgcatgga tcttcggcat aggtaagaac
    13381 atcattcagc cctttgcata gagaaatggc ttcatcaagt agccaggcac cgttgggatc
    13441 aacggtaatc cgcgcatcag ggaagcgttt cttcaatgca cgaacagtgt cgatttcttg
    13501 ctcgccaggt aacaccccgc ccttaagttt gaaatcttta aagccatagc gatcctgtga
    13561 ggcttccgcc agacgcacaa cggcttcgct gttcatcgct ttctgatggc gcaactgata
    13621 ccactcatgg ttgcccggcg tattttccag ataaggaaga tcggttttgg tccgatcacc
    13681 gatataaaac agataaccga ggacggtaat agtctcgcgt tgcttgcctg gccccaacag
    13741 ttcgcagacc ggaacattca gcgccttacc tagcaggtca agcaaggcgg cttccagcgc
    13801 cgccacggca ttaacgcgca attcaaaagt ccaggcacct ttgccgaagg tatcaaaatc
    13861 ggctgcctga ttacctttat gcacctgctg aaccacttta ttcaggcgcg caacttcctg
    13921 gcccagaacc atcggaatag catcgaccag tgtttgataa atcacctctc cgcccggcgc
    13981 ttcaccaatg ccggtatgcc cggcgttatc ggtgagtacc acaatattgc gggtgaaata
    14041 tgcgttatgt gccccaccaa tattaagcaa catgctatcg tgcccggcga ccggaatgac
    14101 tttcatatca gtaataacag gactggattg tgtcgtcatc ttattgcccc gcgacaggtt
    14161 tcagctcgat acgcttgata tcgcccacca gcaccaggta gctcagtacc gcgattaagg
    14221 catgaacacc aacataaatc agcgccccat taaaggagcc agtcgtgcca acgatataac
    14281 caattgcgat tggcgtgacg atgccagaaa tgttaccgaa catgttgaac aggccaccgg
    14341 aaagaccgct gatctctttt ggcgcggtat ctgccattac tgcccaaccc agcgccccga
    14401 tgcccttacc gaagaaggcc agcgccataa agccgatgat catccactca acgttgacgt
    14461 agttgcagaa caccatcacc atcgacagca acatgcccat tacgatcggt gttttacgcg
    14521 caatgttcag cgatcccgtg cgacgcatca gccagtcgga aataatccca cccagcacac
    14581 cgccgataaa accgcaaacc gccggaacgg aagccacaaa gcccgctttc agaatcgaca
    14641 tcccacgcgc ctgcaccaga taaaccggaa accaggtaat gaaaaagtaa gtcagggcgt
    14701 tgatacagta ctgaccgata taaacgccga tcatcatccg tgacccgagc agttgtttga
    14761 tctgccccca cttcacgctg aacggcactt taactttggt gttttgctga tccatattga
    14821 tcagcgcgcc acccgcggcg atgtactcca gctctttctg atttaccccc ggatgttgat
    14881 ttggctcgtg gatgactttc aaccagataa agctgatgac aatccccaga ccgcccataa
    14941 agaagaagac gtgtgaccag cccacttcat gcgtcagcca gcccataatc ggcgcgaaga
    15001 tcaccgttgc gaagtattga gcggagttaa aaatcgacac cgccgttccc ctttcctgcg
    15061 ccggaaacca ggccgcaaca atgcgactgt tgccggggaa agatggcgct tcagcaagcc
    15121 cgaccaggaa gcgcagagta aacagggcaa cgataatgcc gaatccacta aagatatcga
    15181 cgaagccttg cagcaaggta aacatcgacc agataaagat cgaccagaag tagacgcgtt
    15241 ttgaaccaaa acgatccagc aaccaaccac cagggatctg cccgataaca taagcccatg
    15301 agaaggcaga gaacacatag cccattccca cgggatcaag gccgatatct ttggccattt
    15361 ccgaaccggc aatagaaagc gtagcgcggt cgccgtagtt gaaggatgtg acgataaaca
    15421 acatcaccac tatccagtaa cgagcattcg tgcgtttttc cacactgctc gcagcctgac
    15481 ttaaagaact cattgttgca ctcctgaaaa ttcgcgttag ccacgctcac tctggactgc
    15541 gacatagcca ggaaatcaga ggtgacgtag ggtgtttttt gccgttttta taggtcgttc
    15601 gccgaatacg gcgcgtgttt atatctggca atagcagtat aaaaagcgcg ccatagcggc
    15661 tcaccgtgca acaacacaac attaatgcgt tcaatgaggc ccgattttgg cattagccct
    15721 ggacggtgga atccacttca cggaaatgaa aacaagaaca agaaaggaag ggttaaaacg
    15781 aagaaataaa gaagagtatg aaatggatcg cttgactcca ggcaaacgcc agtaaaaatc
    15841 cgcgctatga agcagttttt actggcattt gcctgaaaag attcgattca gcaccgctaa
    15901 aacgacattt accgctcgct gaacatatca ggacaacagc gtgccccact gttcgaccca
    15961 cggatttgat tccgtttccg gttccgggtt ctcactggcg tcaatcaaca gcatttcgcc
    16021 aacccgctga gcgctctgtt cctgcaacaa ggcatcgaac tgtttgccgc cattgcagaa
    16081 atttacataa ctgctgtcgc cgagcgcaat cacgccataa cgcagattcg gctggaagcc
    16141 cagactatct ttgattccct gaaagagcgg cacaatgcta tcaggaaggt cgccctgccc
    16201 ggtcgtggac gtaaccacca gaacatactt atcctgataa ggcagccagt cgcttaattc
    16261 aggatcttca aataccgttg ctttgtggcc ctgtgcggtc agaattgctt ctgcttcttc
    16321 cgccactaac agcgaatttc cgtacatggt gccgacaaaa ataccaattt ccgccatacc
    16381 gttttctccc tggattagga acttatctct ccatcctgac ccgatggcgc actgaactca
    16441 accctttcat tttcaggaag cagaccgcgc cagccaaatt gtgataacgc ctgcatccag
    16501 gtgtcgtcca ggcccgcgtg aatagtcagc ggctcgccag taaaaggatg cgtcaatgac
    16561 aactgactgg catgtagcat taaccgctgg aggccaaaat gctcagcacc gctgcgattc
    16621 tggcgtaaat cgccatgttt gctatcgcca ataatcggat gacgcaaatg ggcaagatgt
    16681 cggcgaagct gatgtttgcg tccggttttc ggctccagtt ccaccaggcc gtagcgcgtg
    16741 gtcgggtaac gtccggtcgc taccggcatt tctacggtcg ccagaccgcg ataatgcgtc
    16801 actgctggct gcgggccttt atcttcgcgg gcaaatttat cagcgatttt gtccagttct
    16861 tccaccagtg gataatccag caccgcttct tccatcaacc agccgcgcac aatcgcatgg
    16921 taacgtttct ggatttggtg ctgttcaaac tgttgtgcca gcagccgtcc ggcctcgctg
    16981 gataatccca tcaacaacac accagaagtg ggtcggtcca gacgatgagc agtaaaaaca
    17041 tgctgtccta tctggtcacg cacggtttgc atgaccacta ctttctcgtc gcgatccagc
    17101 cagctacggt gaaccagcca gccggagggt ttatttaccg caaccagcca ttcatcctga
    17161 tagagtattt ccagcattag ctcgcatcat ccgcaaaaag agcatccagt ttttccaact
    17221 cagccagaat aagcgcgcgt tgcggatggt ccgtcgccag cgccatttca taatagggtg
    17281 caacagcaaa agcgcccggt aacggctggt tgttatttaa caaatcgtgc attcgcggga
    17341 tcagcaccca ctgcaaccac tccagtggtt ccatggtgtc cataaagaac ggttgggtac
    17401 tattaaattg atgcggctgg ggttcatcgt ttcgccagtg ctgatgttca cgcagtaacg
    17461 cttcaagcgt ctgcaactgg aaacgaacgc ggtcatgagt ggtcataaag caacttcaat
    17521 aagaaaaact gccgcgaagg atagcaggaa taaaaaaagg gagcactgta ttcacagtgc
    17581 tcccggttcg tttcgcagca ttccagctac ttttgttgct ccctgctcat ccttgacaac
    17641 ttttcctctg gccttgcggc caatcgttca tcctgaacta ttgcttcctg ctcacaccac
    17701 cccgatgtga tacttcatcc tgaagtgtcc ctggccttcc tgacccaccg aatcatcctg
    17761 accggttctc attctccatc ctggaggtgt cctttaacgc gtcctgcgtc atcctcttcg
    17821 cttcatccag aagcctttcc ctgaaacacc atcctggtgt gtcctgcaga agtgtcatca
    17881 tcctgatgtt cacttcgttg tctgactccc tgtcgacgaa gatagaatcg tctttttcgc
    17941 gaagtcttac aaggcgctta cagacaatgc cttaaggaaa atttcatatg aaagttaaga
    18001 attaacaatc aacattttga ttttaaatga aaataacgat tcttatcttt ttaagtattc
    18061 gcttatttcc tggcgatctc ctacagggtc tgtaagagat ctctcacaac cagatggggt
    18121 atttggatta cagaagaggc ttaagttgat taaggaattc cgcaagattt gaggctaaat
    18181 gcgtccgttt acgtgttccc agcgtctctt tgcacacttc accggagaga ttacacaccg
    18241 agatgacctc taactcttct tccagcgtcg cgataaatag cgtgggcggc agttttagcc
    18301 ttttctgagt caccagatga ccaatcagat tttcctgcac ccgacggaag tcgtcttcac
    18361 tccaggtttg cagcaatgtc agtttgatat cgccaaactg cgcgtgcata tccccggcaa
    18421 actgagtcgt ataaaaggta tgaattgtgg gttgtatcac aatatcaaaa gcgcgttcaa
    18481 ccgcgtttac attttgttcc ccggtaaacg gctggggttg ccagtatacg gcatcttcgg
    18541 tagttgaaat aatgcacggt gaaggaacac cgtacagttc ctcacttaac ggccagcttt
    18601 tatgctcttc atgccacgca tcgcagtagc gtgcagtaaa atctttcagg gcttgtgcgg
    18661 tcaaatcgtc cactaatttc tctcttcact taaaccagat acacttgtcg tttagtttat
    18721 ctggtttatg acggtgaaac atgtcttctt atgcaaacca tcaggcactt gcgggcctga
    18781 ctcttggaaa atcaaccgat taccgggata cctatgacgc cagcctactg caaggcgttc
    18841 cacgcagcct gaatcgcgac ccgctgggtc tgaaagcgga taacctgcct tttcacggta
    18901 cggatatctg gacgctgtat gaactttcct ggctgaatgc gaaaggtttg ccgcaggtcg
    18961 ctgtcggtca tgttgaactt gattacacca gcgtaaatct gattgagtcg aagagtttta
    19021 agctctatct caacagtttt aaccagacgc gttttaataa ctgggatgag gtgcgccaga
    19081 cgctggagcg cgacttaagc acttgcgctc agggtaagat tagcgtggcg ttatatcgtc
    19141 ttgatgaact ggaaggccag ccgataggtc attttaatgg cacttgcatt gatgaccagg
    19201 atatcactat cgataactat gaattcacta ctgactatct ggagaatgcc acctgtggtg
    19261 aaaaagtagt ggaagagacg cttgtcagcc acctgctgaa atcaaactgc ctgatcaccc
    19321 atcaaccaga ttggggttcg atccaaattc agtatcgtgg acgccaaatt gacagagaaa
    19381 aactgctgcg ttacctggtc tcattccgtc atcacaacga gttccacgaa cagtgcgtgg
    19441 aacgcatctt taatgacctg ttacgcttct gccagccaga aaaattgagc gtttacgcac
    19501 gttatacccg tcgtggcggt ctggacatta acccgtggcg cagtaatagc gattttgtcc
    19561 catcgaccac aagactggtt cggcaataaa ttttttctca attttgcgtg ctggattcac
    19621 gcagaaggtt gtgaaaggac atcaggcagg gctattgtaa tcaaagggaa tgacgatatt
    19681 cgtcccataa ggagtttttc ttgattacac atattagccc gcttggctcc atggatatgt
    19741 tgtcgcagct ggaagtggat atgcttaaac gcaccgccag cagcgacctc tatcaactgt
    19801 ttcgcaactg ttcacttgcc gtactgaact ccggtagttt gaccgataac agcaaagaat
    19861 tgctgtctcg ttttgaaaat ttcgatatta acgtcctgcg ccgtgaacgc ggcgtaaagc
    19921 tggaactgat taatcccccg gaagaggctt ttgtcgatgg gcgaattatt cgcgcgttgc
    19981 aggccaactt gttcgcggtt ctgcgtgaca ttctcttcgt ttacgggcaa atccataaca
    20041 ccgttcgttt tcccaacctg aatctcgaca actccgtcca catcactaac ctggtctttt
    20101 ccatcttgcg taacgctcgc gcactgcatg tcggtgaagc gccaaatatg gtggtctgct
    20161 ggggcggaca ctcaattaac gaaaacgagt atttgtatgc ccgtcgcgtc gggaatcagc
    20221 tgggcctgcg tgagctgaat atctgcaccg gctgtggtcc gggagcgatg gaagcgccga
    20281 tgaaaggtgc tgcggtcgga cacgcacagc agcgttacaa agacagtcgt tttattggta
    20341 tgacagagcc gtcgattatc gccgctgaac cgcctaaccc gctggtcaac gaattgatca
    20401 tcatgccaga tatcgaaaaa cgtctggaag cgtttgtccg tatcgctcac ggcatcatta
    20461 tcttccctgg cggtgtgggt acggcagaag agttgctgta tttgctggga attttaatga
    20521 acccggccaa caaagatcag gttttaccat tgttcctcac cggcccgaaa gagagcgccg
    20581 actacttccg cgtactggac gagtttgtcg tgcatacgtt gggtgaaaac gcgcgccgcc
    20641 attaccgcat catcattgat gacgccgctg aagtcgctcg tcagatgaaa aaatcgatgc
    20701 cgctggtgaa agaaaatcgc cgcgacacag gcgatgccta cagctttaac tggtcaatgc
    20761 gcattgcgcc agatttgcaa atgccgtttg agccgtctca cgagaatatg gctaatctga
    20821 agctttaccc ggatcaacct gttgaagtgc tggctgccga tctgcgccgt gcgttctccg
    20881 gtattgtggc gggtaacgta aaagaagtcg gtattcgcgc cattgaagag tttggtcctt
    20941 acaaaatcaa cggcgataaa gagattatgc gtcgtatgga cgacctgcta cagggttttg
    21001 ttgcccagca tcgtatgaag ttgccaggct cagcctacat cccttgctac gaaatctgca
    21061 cgtaatctcc ctcccccggt gacgtttgtt gccgggtttt ctcgtttttg gtcacatacc
    21121 catcaattta tttcatttgt tatatgaatg tttcttacca ccctcacgcg ataaatatca
    21181 ttgcagttaa tatgtcatac aatttatgtt gtaacgcaaa cgtttcccta ttttcataaa
    21241 ccacatttta tcgctatgaa aaagaaatta tcgccatgat taactaaaag tattgatttt
    21301 ttcagttcaa cctacatata ttgcgcgccc cggaagaagt caaatgtcgt ttaatgggca
    21361 aatattgccc ttaaattctc ttttactttt gatttacaga gtaaagcgtt gggataatct
    21421 atcttccaag tagattattg tatttgggat caagatcact gatagataca taacttgtgt
    21481 gtatctttcc gccctcaaat tattacggcg gtaaatgatt aagccatcgc cgatagacag
    21541 atttcatttt tacggtcagg caccttcccg agctgaactg gctaaaagct gaattatttg
    21601 cattcctcca ggagaaatag atggaaacga ctcaaaccag cacgattgcg tcgaaagact
    21661 ctcgtagtgc ctggcgcaag acagacacca tgtggatgct gggcctttac ggcacggcaa
    21721 tcggcgcggg cgtgctgttc ctgccaatca acgccggtgt tggcggtatg atcccgctga
    21781 tcatcatggc tatccttgcg ttcccgatga cattttttgc tcaccgcggc ctgactcgct
    21841 tcgtactgtc tggtaaaaac ccaggcgaag acatcaccga ggttgtagaa gaacactttg
    21901 gtattggcgc aggtaaactg attaccctgc tctacttctt cgctatctac ccgatcctgc
    21961 tggtttatag cgtggcaatc accaatacag ttgaaagctt catgtctcac cagctgggta
    22021 tgacaccacc gccgcgtgcg attctgtcgc tgatcctgat cgtgggtatg atgaccatcg
    22081 ttcgcttcgg tgagcagatg atcgttaaag caatgagtat tctggtattc ccgtttgttg
    22141 gcgtactgat gctgctggct ctgtacctga tcccgcagtg gaacggcgca gcactggaaa
    22201 cgctgtctct ggacactgca tctgcaaccg gaaacggtct gtggatgacc ctgtggctgg
    22261 caattccggt tatggtgttc tctttcaacc actctccgat catctcttct ttcgccgttg
    22321 cgaagcgtga agagtacggc gatatggcag aacagaaatg ctcgaagatc ctggcattcg
    22381 cacacatcat gatggtgctg accgtaatgt tcttcgtctt cagctgtgta ctgagcctga
    22441 ctccggcaga cctggctgcg gctaaagagc agaacatctc gattctgtct tacctggcta
    22501 accactttaa cgcaccggtt atcgcgtgga tggctccgat tatcgcgatt atcgctatca
    22561 ccaaatcctt cctcggccac tacctgggcg cacgtgaagg cttcaacggt atggtgatta
    22621 aatctctgcg tggtaaaggt aagtctatcg aaatcaacaa gctgaaccgt atcactgcgc
    22681 tgttcatgct ggtaacgacc tggattgttg ccaccctgaa cccgagcatc ctgggtatga
    22741 ttgaaaccct gggcggccca atcatcgcga tgatcctgtt cctgatgccg atgtacgcaa
    22801 ttcagaaagt accggcaatg cgtaagtaca gcggtcacat cagcaacgta ttcgttgtcg
    22861 tgatgggtct gattgcaatc tccgcaatct tctactctct gttcagctaa gtcctttcgc
    22921 gccgctttcg ggcggcgctt cctccgtttt aacgcgatgt atttcctatg attagcgtat
    22981 tcgatatttt caaaatcggc attggccctt ccagttctca taccgttgga ccaatgaaag
    23041 cgggtaaaca atttaccgac gatctgattg cccgtaatct gcttaaagac gtgacccgcg
    23101 tggtggttga cgtgtacggc tcgctctctc tgaccggtaa aggccaccac actgatatcg
    23161 ccattattat gggcctggcg ggtaacctgc cggataccgt ggatatcgat tccatccctg
    23221 gttttattca ggatgtgaat actcatggtc gcctgatgct ggcaaacggt cagcatgaag
    23281 tggagttccc ggttgatcag tgcatgaact tccatgcaga caacctttct ctgcatgaaa
    23341 acggtatgcg cattaccgcg ctggcgggcg ataaagtcgt ttacagccag acttactact
    23401 ctattggcgg tggctttatc gttgatgaag agcattttgg ccagcagaat agcgcaccgg
    23461 ttgaagttcc ttatccgtac agttcagcag ccgatctgca aaaacattgt caggaaaccg
    23521 ggctgtcact ctctggcctg atgatgaaaa atgagctggc gctgcacagc aaagaagagc
    23581 tggaacagca cctggcgaac gtatgggaag tcatgcgtgg cggtattgag cgcggtattt
    23641 ccaccgaagg cgtgttgcct ggcaaactgc gcgttccacg ccgtgctgcg gcactacgcc
    23701 ggatgctggt cagccaggat aaaaccacca ctgacccgat ggcggttgtt gactggatca
    23761 acatgtttgc actggcagtg aacgaagaga acgctgctgg cggacgcgtg gtgactgcgc
    23821 cgactaacgg tgcgtgcggg attatcccgg cagtgctggc gtactacgac aagttcatcc
    23881 gcgaagtgaa cgctaactca ctggctcgtt acctgctggt agccagtgcc attggttctc
    23941 tttataagat gaacgcgtca atttctggtg ctgaagtcgg ctgccagggt gaagttggtg
    24001 tggcgtgctc aatggcggcg gctggtctgg cagaactgtt aggtgcaagc ccggcgcagg
    24061 tgtgcatcgc ggcggaaatc gccatggagc acaacctcgg tctgacgtgt gacccggtcg
    24121 ccggacaggt ccaggtgcca tgcatcgagc gtaacgccat tgcggcagta aaagcggtga
    24181 acgccgcacg tatggcgcta cgccgtacca gcgagccgcg cgtctgcctc gataaagtta
    24241 tcgaaaccat gtacgaaaca ggtaaagata tgaacgccaa gtaccgcgaa acctctcgcg
    24301 gcggcctggc aatgaagatc gttgcctgcg attaatcgct ctccagaggc ctcgttttgc
    24361 gaggcctctt cccgatttct catccagccg tagcctgttc cggcatcgaa tgttaccctt
    24421 atcgcctgat ctttaagggg gttatcgtgg ctgttcattt gcttattgtc gatgcactga
    24481 atcttattcg tcgcattcat gccgttcagg gatcgccctg tgtcgaaacc tgccagcatg
    24541 cgctcgatca gctcattatg cacagccagc caacccacgc ggtcgccgtt tttgatgatg
    24601 aaaaccgcag tagcggctgg cgtcatcagc gtttaccaga ttacaaagcg ggtcgaccgc
    24661 caatgccgga agagttgcac gacgagatgc ctgcattacg cgccgccttt gagcaacgcg
    24721 gcgtcccgtg ctggtcaacc agcggcaacg aagccgatga cttagccgcc acgctggcgg
    24781 tcaaagtgac acaggccggg catcaggcaa cgattgtttc gacagataaa ggctactgtc
    24841 agttactttc accgacatta cgtattcgtg attacttcca gaaacgttgg ctggatgcgc
    24901 catttatcga taaagaattt ggcgttcaac cgcagcagtt gcccgattac tggggacttg
    24961 cggggatcag cagttcaaag gtaccgggtg ttgcgggaat cggaccaaaa agcgccacgc
    25021 agctgctggt cgagtttcag agtctggaag ggatatatga gaatctggat gcggttgccg
    25081 aaaagtggcg caaaaaatta gaaacccata aagagatggc gtttctgtgc cgcgatattg
    25141 cccgcttaca aaccgatttg catatcgacg gcaatttaca gcaattgcgg ttggtacggt
    25201 aacggcgagc cggatgcaaa cgattatcaa cacattgaat ttaatgcact tatataccgg
    25261 ataagacgcg gctagcgtcg tatccggcat tatcacatca gcgcatttac caggcggtat
    25321 ggtaaagctc tacaatatcc tcaagcgttg cttcacgcgg gttgccaccg gtacaaacat
    25381 catccagtgc cgcctgcgcc agtgccggaa tgtcttcctt gcgtacacca acatcacgca
    25441 aatgtggcgg aataccgaca tcacggttga gagcaaacac cgcttcaaca gcggcattac
    25501 gcgcctcttc caggctcata ccttccactt tcacgcccat aacgcgcgcg atatcgcggt
    25561 acttctcacc ggtaaagtca gcgttatagc gcatgacatg cggtaacagg atggcgttcg
    25621 caacaccgtg tggagtgttg taaaacgcgc ccagtggatg cgccatacca tgcaccaacc
    25681 ctaacccaac attcgagaag cccatacccg caacatactg cccgagcgcc atttcttctc
    25741 cggcatcctt atcaccagca accgatcctc gcagcgcccc agcaatgatt tcaatcgctt
    25801 taatgtgcag tgcatcggtt agcgcccacg cgccacgggt aatatacccc tcaatagcat
    25861 gagtgagcgc atcgacaccc gtcgcagctt tcagcgctgg aggcatacca tccatcatgt
    25921 cagcgtcaat aaacgccacc tgcgggatat catgcggatc aacgcaaaca aacttgcgcc
    25981 gtttctcttc gtcagtgatc acgtagttaa tggtcacttc tgccgcagta cctgctgtgg
    26041 taggaattgc cagaatcggt acactgggtt tattggtcgg ggaaagccct tccaggctac
    26101 gcacatcggc aaactccggg ttgttgctga taatgccaat cgctttacaa gtatcctgtg
    26161 gagaaccacc accaatagcg atcaggtaat ccgcgccgct attctggaat acaccgagcc
    26221 cttctttgac gacagtaatt gttgggttgg gcactacgcc gtcgtaaatc gcccatgcca
    26281 gccctgcagc atccatctta tcggtcactt tcgccaccac gccgcattgc accagcgttt
    26341 tatcggtgac gatcagcgcc ttctgataac cacggcgttt cacctcatcg gttaaagccc
    26401 caacagcacc ccgaccaaac catgccgttt cgttcagaat cattctgtta gccatcatcc
    26461 ttctccttgt tgctttacga aattactctt caatccgtaa cccataggtt ttgaatttct
    26521 ccagcactac ggcaatctct tcatcgctca gcactggcac cgggtccgta atcgccaggg
    26581 tcgtcacgta aagttgcgcc agcacttcaa cttcatgcgc cagccataac gctttttcca
    26641 gattcacctc acaagcgata agcccatgat gttgtaacaa agttgcctta cgatttttga
    26701 gagccagcgc aacatgttca gaaagttcgc gtgttccaaa ggtcgcataa ggcgcgcaag
    26761 gaatagaatt accacctgcc gccgcaatca tgtagtgaat agcggggatc ggtcggttaa
    26821 gaatggaaac tgctgtgcaa tgaacggcat gattgtgaac aaccgcgttg gcatccggtc
    26881 tgctttgata ggccgccata tggaaacgcc attcgcttga ggggagcttt ccttcctcat
    26941 gtttaccgtt gccatcaata aagacaatat gcgactccgt cagtttttca tatggaatgc
    27001 ctgtaggcgt aatcagcatc ccatcctgat aacgtacact gacgttcccc gctgtcccct
    27061 ggttcagtcc caggcgggtc atttccaggc aagtgtcaat aatctgacga gcaagtttat
    27121 ttcgttccat tagctacctc tctctgattc aaaacagggc aataatgttg ttcctttcac
    27181 actattgaat tagccgttta attacccacc atcttcttcc tgattaacaa gaaagaaatt
    27241 cacaagctta tattttgtga cctagctcaa ctaatcacag taaataactg caagttctct
    27301 ttttataacc ccattaaaaa tgaccgctct taaaaatatt tatcaaaacg gtcatttttc
    27361 tattcaccca agccaggaat gcccgttttc ggcataaaca attaatacag tcatctggtt
    27421 tgtgtttttt atgatttatt ttctgaaacg ggcatgaaat ttcgattatt aaagtgatgg
    27481 tagtcacata aagtcacctt ccagctaata agtgtgaccg ccgtcatatt acagagcgtt
    27541 ttttatttga aaatgaatcc atgagttcat ttcagacagg caaatattca ctgatatgaa
    27601 gcccgaactc gctggttttg cacttttgaa aacataaccg attacgtgct taagcttctg
    27661 aacctaagag gatgctatgg gaaacacatc aatacaaacg cagagttacc gtgcggtaga
    27721 taaagatgca gggcaaagca gaagttacat tattccattc gcgctgctgt gctcactgtt
    27781 ttttctttgg gcggtagcca ataaccttaa cgacatttta ttacctcaat tccagcaggc
    27841 ttttacgctg acaaatttcc aggctggcct gatccaatcg gccttttact ttggttattt
    27901 cattatccca attcctgctg ggatattgat gaaaaaactc agttataaag caggaattat
    27961 tactgggtta tttttatatg ccctgggcgc tgcattattc tggcccgctg cagaaataat
    28021 gaactacacc ttatttttag ttggcctatt tattattgca gccggattag gttgtctgga
    28081 aactgccgca aacccttttg ttacggtatt agggccggaa agcagtggtc acttccgctt
    28141 aaatcttgcg caaacattta actcgtttgg cgcaattatc gcggttgtct ttgggcaaag
    28201 tcttattttg tctaacgtgc cacatcaatc gcaagacgtt ctcgataaaa tgtctccaga
    28261 gcaattgagt gcgtataaac acagcctggt attatcggta cagacacctt atatgatcat
    28321 cgtggctatc gtgttactgg tcgccctgct gatcatgctg acgaaattcc cggcattgca
    28381 gagtgataat cacagtgacg ccaaacaagg atcgttctcc gcatcgcttt ctcgcctagc
    28441 gcgtattcgc cactggcgct gggcagtatt agcgcaattc tgctacgttg gcgcacaaac
    28501 ggcctgctgg agctatttga ttcgctacgc tgtagaagaa attctaggta tgactgcagg
    28561 ctttgccgct aactatttaa ccggaaccat ggtgtgcttc tttattggtc gtttcaccgg
    28621 tacctggctc atcagtcgct tcgcaccaca caaagtcctg gccgcctacg cattaatcgc
    28681 tatggcactg tgcctgatct cagccttcgc tggcggtcat gtgggcttaa tagccctgac
    28741 tttatgcagc gcctttatgt cgattcagta cccaacaatc ttctcgctgg gcattaagaa
    28801 tctcggccag gacaccaaat acggttcgtc cttcatcgtt atgaccatta ttggcggcgg
    28861 tattgtcact ccggtcatgg gttttgtcag tgacgcggcg ggcaacatcc ccactgctga
    28921 actgatcccc acactctgct tcgcggtcat ctttatcttt gcccgtttcc gctctcaaac
    28981 ggcaactaac tgaacatatt ttccgaataa agtgaggaat ctgtaatgaa aaaaatcagc
    29041 ttaccgaaaa ttggtatccg cccggttatt gacggtcgtc gcatgggtgt tcgtgagtcg
    29101 cttgaagaac aaacaatgaa tatggcgaaa gctacggccg cactgctgac cgagaaactg
    29161 cgccatgcct gcggagctgc cgtcgagtgt gtcatttccg atacctgtat cgcgggtatg
    29221 gctgaagccg ctgcttgcga agaaaaattc agcagccaga atgtaggcct caccattacg
    29281 gtaacgcctt gctggtgcta tggcagtgaa accatcgaca tggatccaac ccgcccgaaa
    29341 gccatttggg gctttaacgg cactgaacgt cccggcgctg tttacctggc agcggctctg
    29401 gcagctcaca gccagaaagg catcccggca ttctccattt acggtcatga cgttcaggat
    29461 gccgatgaca catcgattcc tgccgatgtt gaagaaaaac tgctgcgctt tgcccgcgcc
    29521 ggtttggccg tcgccagcat gaaaggtaaa agctatctgt cgctgggcgg cgtttcgatg
    29581 ggtatcgccg gttctattgt tgatcacaac ttctttgaat cctggctggg aatgaaagtc
    29641 caggcggtgg atatgaccga actgcgtcgc cgtatcgatc agaagattta cgacgaagcc
    29701 gaactggaaa tggcactgac ctgggctgat aaaaacttcc gctatggcga agatgaaaat
    29761 aacaaacagt atcaacgcaa tgccgagcaa agccgcgcag ttctgcgcga aagtttactg
    29821 atggcgatgt gtatccgcga catgatgcaa ggcaacagca aactggccga tattggtcgc
    29881 gtggaagaat cacttggcta caacgccatc gctgcgggct tccaggggca acgtcactgg
    29941 accgatcaat atcccaatgg tgacaccgcc gaagcgatcc tcaacagttc atttgactgg
    30001 aatggcgtgc gcgaaccctt tgtcgtggcg accgaaaacg acagtcttaa cggcgtggca
    30061 atgctaatgg gtcaccagct caccggcacc gctcaggtat ttgccgatgt gcgtacctac
    30121 tggtcaccag aagcaattga gcgtgtaacg gggcataaac tcgatggact ggcagaacac
    30181 ggcatcatcc atttgatcaa ctcaggttct gctgcgctgg acggttcctg taaacaacgc
    30241 gacagcgaag gtaacccgac gatgaagcca cactgggaaa tctctcagca agaggctgac
    30301 gcttgcctcg ccgctaccga atggtgcccg gcgatccacg aatacttccg tggcggcggt
    30361 tactcttccc gcttccttac cgaaggcggc gtcccgttca ccatgactcg tgtcaacatc
    30421 atcaaaggcc tgggatcggt actgcaaatc gcggaaggct ggagcgtgga attgccgaag
    30481 gatgtgcatg acatcctcaa caaacgcacc aactcaacct ggccaaccac ctggtttgca
    30541 ccgcgcctca ccggtaaagg gccgtttacg gatgtgtact cggtaatggc gaactggggc
    30601 gctaaccatg gggttctgac catcggccac gttggcgcag actttatcac tctcgcctcc
    30661 atgctgcgta tcccggtatg tatgcacaac gttgaagaga ccaaagtgta tcgtccttct
    30721 gcctgggctg cgcacggcat ggatattgaa ggccaggatt accgcgcttg ccagaactac
    30781 ggtccgttgt acaagcgtta atacattact ccctgatgtg atgcccggtc gctccggcta
    30841 ccgggcctga acaagcaaga gtggttagcc ggataagcaa tgttatccgg ctatattgca
    30901 ggagcgatta tgaaacaaga agttatcctg gtactcgact gtggcgcgac caatgtcagg
    30961 gccatcgcgg ttaatcggca gggaaaaatt gttgcccgcg cctcaacgcc taatgccagc
    31021 gatatcgcga tggaaaacaa cacctggcac cagtggtctt tagacgccat tttgcaacgc
    31081 tttgctgatt gctgtcggca aatcaatagt gaactgactg aatgccacat ccgcggtatc
    31141 gccgtcacca cctttggtgt ggatggcgct ctggtagata agcaaggcaa tctgctctat
    31201 ccgatcatta gctggaaatg tccgcgaaca gcagcggtta tggacaatat tgaacagttg
    31261 atctccgcac agcggttgca ggctatttct ggcgtcggag cctttagttt caatacgtta
    31321 tataagttgg tgtggttgaa agaaaatcat ccacaactgc tggaacgcgc gcacgcctgg
    31381 ctctttattt cgtcgctgat taaccaccgt ttaaccggcg aattcactac tgatatcacg
    31441 atggccggaa ccagccagat gctggatatc cagcaacgcg atttcagtcc gcaaatttta
    31501 caagccaccg gtattccacg ccgactcttc cctcgtctgg tggaagcggg tgaacagatt
    31561 ggtacgctac agaacagcgc cgcagcaatg ctcggcttac ccgttggcat accggtgatt
    31621 tccgcaggtc acgataccca gttcgccctt tttggcgctg gtgccgacca aaatgaaccc
    31681 gtgctctctt ccggtacatg ggaaatttta atggtccgta gcgcccaggt tgatacttcg
    31741 ctgttaagtc agtacgccga ttctacctgc gaactggata gccaggcagg gttgtataac
    31801 ccaggtatgc aatggctggc atccggcgtg ctggaatggg tgagaaaact gttctggacg
    31861 gctgaaacac cctggcaaat gttgattgaa gaagctcgtc tgatcgcacc tggcgcggat
    31921 ggtgtgaaaa tgcagtgtga tttattgtcg tgtcagaacg ctggctggca aggagtgacg
    31981 cttaatacca cgcgggggca tttctatcgc gcggcgctgg aagggttaac tgcgcaatta
    32041 cagcgcaatc tacagatgct ggaaaaaatc gggcacttta aagcctctga attattgtta
    32101 gtcggtggag gaagtcgcaa cacattgtgg aatcagatta aagccaatat gcttgatatt
    32161 ccggtaaaag ttctcgacga tgccgaaacg accgtcgcag gagctgcgct gttcggttgg
    32221 tatggcgtag gggaatttaa cagcccggaa gaagcccgtg cacagattca ttatcagtac
    32281 cgttatttct acccgcaaac tgaacctgaa tttatagagg aagtgtgaaa tgctgaaaac
    32341 aatttcgccg ttaatttctc ccgaactatt gaaagtgctg gcagagatgg gacatggaga
    32401 tgaaattatt ttttccgatg ctcactttcc cgcccattcg atgggaccgc aggtgatccg
    32461 cgctgatggc ctgttggtga gcgacttgct ccaagcgatt atcccattat ttgagctgga
    32521 cagttatgcc ccgccgctgg tgatgatggc agcggtagaa ggtgacactc tcgatcctga
    32581 agtagaacga cgttaccgta atgcgctttc actacaagcc ccgtgtcctg acatcatccg
    32641 catcaatcgt tttgcgtttt atgaacgggc gcaaaaagcc tttgcgatcg ttatcacagg
    32701 cgaacgagcg aagtacggga atattctttt aaaaaaaggg gtaacaccgt aatctcatat
    32761 cggtacgccc gcatgacgcg ggcggttatc gaatgatggg gtgaaaaata tgaaagcggc
    32821 acgccagcaa gcgatagtcg acctgctgct gaaccatacc agcctgacca cggaagctct
    32881 ctctgaacag ctaaaggtca gtaaagaaac cattcgtcgc gatctcaatg aattacagac
    32941 gcagggtaaa attctgcgca atcatggacg cgctaaatat atccaccgtc aaaatcaaga
    33001 cagtggcgat ccctttcaca tcaggctgaa aagccattat gcgcataaag cagatatcgc
    33061 gcgcgaggcg ctcgcgtgga ttgaagaagg gatggtgata gccttagacg ccagttcaac
    33121 ttgctggtat ctggcacgcc agttgcctga catcaacatt caggtcttca ccaatagcca
    33181 tccgatttgc catgaactcg gtaaacgcga acgcattcaa ctgatcagtt ccggcggcac
    33241 acttgagcgc aaatatggct gttacgtcaa tccctcgctg atttcccaac ttaaatcgct
    33301 ggaaatcgat ctgtttattt tttcttgtga agggatcgat agcagcggcg cactgtggga
    33361 ctccaatgcg atcaacgctg attacaaatc gatgctatta aaacgtgccg cgcaatcgtt
    33421 gttattgatt gataaaagta aatttaatcg ttcaggggaa gcccgcatcg ggcatctgga
    33481 tgaggtaacg cacattattt ctgatgagcg ccaggttgca acttctttgg taacagcctg
    33541 acggaaaggg tagcaggccg aagacgacgc cccggccttg cctgttatcg ctcgtcgcga
    33601 cgaccaccca ccgcagccca gatgcggcgg acgtgcaccg tcacttcttc gcgatcgtga
    33661 tacaactgcc gcgcctgaat ctgagcattt atgccatgtt catcaagctg tgcctgaata
    33721 tacgccagat tgtgtgatac ttcttcgtag cgttttttca tcggcagttt gagattgaaa
    33781 atggtttcac ggcaccagcc attaaccagc cactgcgcca tcaatgccgc aactttagcc
    33841 ggtttttcaa ccatatcgca taccatccag gagatattgc tgcgtgtcgg acggaatttg
    33901 aaaccgtctt cccgcagcca cgtcacctgc ccggtatcca tcagactttg cgccatcggg
    33961 ccgttgtcga cggaataaac ccacatgttg cgcttcacca gttggtaggt ccagccgcca
    34021 gggcaagcgc ctaaatccac cgcccacatc ccgttcgcca ggcgttcatc ccactcatcc
    34081 gcaggaataa acacatgaaa tgcctcttcc agtttgagcg tggaacgact cggcgcctct
    34141 gccggaaatt tcaggcgcgg aatgcccata tagaacggcg aattattgtt gctgtatgag
    34201 taaccggtat agcagcagcc tggtgcaata aagaatacat gcacaaccgg acgcttcggc
    34261 gtttcatagt tcgccagcac cccggcatcg cgcaaggcag cgcgtagcgg cacggtgaat
    34321 ttacggcaga atttcagcaa ctctttgctt tcgttggtat cggcaacttc aacacgcagt
    34381 tcaccgccct tctctactac gccctgcaac atgtcgacaa tgggggtaat acgatcttct
    34441 ggcggcaaat gctgcaagag ttcccccacc acaaaccact ggcgggcaaa aattaatgaa
    34501 ctgaacggca gctcacggat taacttatcg ccatcatcag gttgataaca ttcataaatg
    34561 acataacccg cattctcttt cacgcgggca aaaccgaaaa tttcccgctg gccggcttta
    34621 tcggtaattt ctgcggcgca ctctttttca aagcccggac ggcacagcaa tacaacctta
    34681 ttcatgactt acgcccttgc actttaaacg gatagcacca actaacatta acgcccagcc
    34741 cgcgaggaag ctcacgccgc caaccggtgt gacaaatgcc cacaaacgca gatgggacag
    34801 cgccaggcaa taaaggctgc cgctgaacaa caccgtgcct aacgcgagga aaacgctact
    34861 ccagtaaaac cagatactga tgcgacgctg cattgccacc gccagaccta agatcgccag
    34921 cgtatgaaac gcctggtatt cgaggccggt ctggatccag cccatctcaa cggcccccat
    34981 ggttttactt aacacatgcg cgccaaaagc gcccagagcc acaaaaatga agccgctaat
    35041 ggcggcgaaa atcagcataa aacggctggt catggtcgta ccctacgtaa attattgttc
    35101 ataacgaaag cggaattttt cttgttcagc agcggctttc gccaggatcc attggcgaaa
    35161 ggcggctatt ttacccagtt ctgcctggct gtcatgacaa accagataaa aagcgttttt
    35221 actgaccaga acatcattaa acgggcaaac aagacgtccg gcctcgattt cagattgcgc
    35281 catcacgtta tttgccagcg ccactccctg cccgtgaata gccgcttgca gcaccatggc
    35341 gctatgacta aaaattggcc cttgctgaac gttgatatga tttaacccca actgtcgggt
    35401 atatgtctgc cagtcacggc gtgacgcatc atgtaataac gtatgtttag ccagatcttc
    35461 cggggtcttc aagggttttt cgcctgtcag cagtagcggc gaacacaccg gcaataaata
    35521 ttcggcgtac agtttttcca cccgtagccc cggccagttg ccccgaccat aaaatatcgc
    35581 cacatcaaca tcatccgcca gcttatcttc ctgacgatca accgcctgga ttcgaacgtc
    35641 aattcccgga taagctgaat taaagctgga aagtcgcgga accaaccaat gaatggcgaa
    35701 actggggagt aaactgaccg tcaacgcccc cttggcgcta cgggcctgga gtttacgcgt
    35761 cgcttcggtt aattgcgaaa atatctcttt gatatcgagg aaatagcttt gcccttcctc
    35821 ggtcagcagg agtgaacgat tacggcggcg gaacagtttt agccccaaaa aatcctcaag
    35881 agacttgatt tgatgactta ctgcggcctg ggtcacgaaa agctcttctg ctgcgcgagt
    35941 gaaacttaaa tggcgtgctg cggcatcaaa aactcgtaag gcatttagcg gtggtaatcg
    36001 tttagacatg gcttttaaac tttgatgtta aattaattta acaattagat cacactgtgt
    36061 aacctattag tttttttaat ctgagccatt ataaattgtc cgttgagctt ctaccagcaa
    36121 atacctatag tggcggcact tcctgagccg gaacgaaaag ttttatcgga atgcgtgttc
    36181 tggtgaactt ttggcttacg gttgtgatgt tgtgttgttg tgtttgcaat tggtctgcga
    36241 ttcagaccat ggtagcaaag ctaccttttt tcacttcctg tacatttacc ctgtctgtcc
    36301 atagtgatta atgtagcacc gcctaattgc ggtgcttttt tttaccttgc gatcgcgaat
    36361 tactgatcca gttcgaccat ctctttcacg tcagtacggt tgatctgctg tttgttgcca
    36421 ttagcgtctt tatacgaaat cataccggta tcgttatcag tctgtggttt gccgtcagag
    36481 acgatggtac gtccgtcatt ggtgtgcatc acatagttcg aaccggaaca ggcgctcagg
    36541 gcaaaagtca gcatacaggc agaaataatt gcggcagtct ttttcatcat cttctcctta
    36601 aagcgattaa taaatcttaa tagtattgat taacaggcta aaattaacgc ctaacactat
    36661 tcagcatatg ttacttacgc ggcttcgcca ggatatccag ataattctga tggttagcac
    36721 tctccttgta tcaaagtgaa ttttgcgtca cgatcggtgc atcaagccga ggagtaccat
    36781 gaacgttttt aatcccgcgc agtttcgcgc ccagtttccc gcactacagg atgcgggcgt
    36841 ctatctcgat agcgccgcga ccgcgcttaa acctgaagcc gtggttgaag ccactcgaca
    36901 gttttacagc ctgagcgccg gaaacgtcca tcgcagccag tttgccgaag cccaacgcct
    36961 gaccgcgcgt tatgaagctg cacgagagaa agtggcgcaa ttactgaatg caccggatga
    37021 taaaactatc gtctggacgc gcggcaccac tgaatccatc aacatggtgg cacaatgcta
    37081 tgcgcgtccg cgtctgcaac cgggcgatga gattattgtc agcgtggcag aacaccacgc
    37141 taacctcgtc ccctggctga tggtcgccca acaaactgga gccaaagtgg tgaaattgcc
    37201 gcttaatgcg cagcgactgc cggatgtcga tttgttgcca gaactgatta ctccccgtag
    37261 tcggattctg gcgttgggtc agatgtcgaa cgttactggc ggttgcccgg atctggcgcg
    37321 agcgattacc tttgctcatt cagccgggat ggtggtgatg gttgatggtg ctcagggggc
    37381 ggtgcatttc cccgcagatg ttcagcaact agatatcgat ttctatgctt tctctggtca
    37441 caaactgtat ggcccgacgg gtatcggcgt gctgtatggc aaatcagaac tgctggaagc
    37501 gatgtcgccc tggctgggtg gcggcaaaat gattcacgaa gtgagttttg acggcttcac
    37561 gactcaatct gcgccgtgga aactggaagc aggaacgcca aatgtggctg gtgtcatagg
    37621 attaagcgcg gcgctggaat ggctggcaga ttacgatatc aaccaggcag aaagttggag
    37681 tcgcagcctg gcgacgctgg cggaagatgc gctggcaaaa cgtcctggct tccgctcatt
    37741 ccgctgccag gattccagcc tgctggcctt tgattttgct ggcgttcatc acagcgatat
    37801 ggtgacgctg ctggcggagt acggtattgc cctgcgggcc gggcaacatt gcgctcagcc
    37861 gctactggca gaattaggcg taaccggcac actgcgcgcc tcttttgcgc catataatac
    37921 aaagagtgat gtggatgcgc tggtgaatgc cgttgaccgc gcgctggaat tattggtgga
    37981 ttaatgacga acccgcaatt cgccggacat ccgttcggca caaccgtaac cgcagaaacg
    38041 ttacgcaata ccttcgcacc gttgtcgcaa tgggaagata aatatcgcca gttgatcatg
    38101 ctggggaaac agcttccggc tttgccagac gagttaaaag cgcaggctaa agagattgcc
    38161 ggatgcgaaa accgcgtctg gctgggatat accgtgactg aaaacggtaa aatgcacttc
    38221 tttggcgata gcgaagggcg cattgtgcgc ggcctgctgg cggtgctgtt gactgccgtt
    38281 gaggggaaaa ccgccgccga attgcaggca cagtcgccgc tggcattgtt tgatgagctg
    38341 ggattgcgtg cgcaacttag cgcctcacgc agccaggggt taaatgcgtt aagcgaggcg
    38401 attatcgctg cgacgaagca ggtttaagca tagaaccggg tcggataaga cgttcgcgtc
    38461 tcatccgacc tgattgttta accctgacgc gccgctttcg ccatcatctt cttcagcgca
    38521 tgagaaaccg caacaaaccc aaaggtggcg gtcaccatcg ttgccgcgcc aaatccagat
    38581 gcacaatcca tccgcttcgg cccttctgcc gtggctttca tcgcacacac agtaccgtct
    38641 gactgcgggt acaccagcgc ttcagtagaa aacacgcaat cgacgccgag cttaccttta
    38701 ctgtttttca ctacgccaaa atcgcttttc aggcgctcgc gcaacttcgc cgccagcgga
    38761 tcctgaatcg ttttcgccag atcggtaacc tgaatctgcg tcgggtcaat ctgcccaccc
    38821 gcgccaccgg tcgtgaccag cgggatttta ttgcgccgac aataagcaat cagcgccgct
    38881 ttgggccgta cactatcaat ggcatcaatc acgtacgaat aacccacgct catatactgc
    38941 gctacgttat ccggcgtcac gaaatcatcc accaccgtta cacggcactc cgggttaatc
    39001 tggcgaatac gctccgccat gacttccgct tttgccagcc cgacgttatc gcgcagagcg
    39061 tgaatttgcc gattggtatt ggtgacgcac acatcatcca tatcgataag cgtgattgcg
    39121 ccaatccccg tgcgcgccag cgcttccgcc gcccaggaac cgacaccacc gataccaacc
    39181 acacaaatat gcgcgtcagc aaacagttgc aacgcttttt caccatacag acgcgctgtg
    39241 ccaccaaaac gctgacgcca tgcatcacta attaccacag acatagcacc tcagaattaa
    39301 aaagggtgag gttatccctc accctgtcgt atccgtaaaa acggtataca gaataacaca
    39361 tcagccgcta aagacgttac ctgcgcccgg ggcggttttc agcacccaga cacgtccata
    39421 gtggttgtac caacctgcgc ggtgtccggc ttccggcccg atcccttgat agatatcgaa
    39481 gtgttggcct ttgattgcac caccgacatc cagcgccacc atcagacgca gttcgtactg
    39541 accattaaat ttgccgttat tatccagcaa cggcacttct gccagcaagg tagtacctgg
    39601 cggaataatg gaacgatcag aggcaactga cgcgcgacca accagcggca ccgcacttgc
    39661 ccctttcacc ggtgcaaaag attgcggttt aaagaagacg aaagacgggt tctgttccag
    39721 cagctcgcga acctcggctt cactgtgtgt ttcgccccag tgacgaatcg cctgcatcga
    39781 catatcttct tttttcactt cgccacggtc gatcagcacc ttaccaatgc tgcgataggc
    39841 atgaccgttt ttccctgcat agctgaaaaa gttaagcgga ctgccatcac caaagtcgat
    39901 atacccacta ccctgcacat ccataatgaa gttatccatc agggagttac tgtaagcgag
    39961 aatatattta tcactcaatg cccccgcgta gatctccgca cgagacggca gacgaccacg
    40021 ttttggcggc atacggtaaa taggatactg gaactcgccc tggcgggtat ggcgcgcctg
    40081 aattaccggc gtgtaatagc ctgtaaactg cacgttacca tagttgtcgg caccttccat
    40141 ctgccaggca tcaatgccga actggcgcat attgcgggta tcaccgcctg cgcgcagcca
    40201 ctcttgcacc gcgttataaa cattactctg gttgccatac agacgcggtg acgaattacg
    40261 gatatggtta atttgctcgg caaaatcacc ggcgttaatc ggcgcgccaa cggcatctgg
    40321 ctggttcacc agagagaaag gctgggtaaa tttcccgtct ttatattgct gtccgcgatc
    40381 ggttggtttg gaagagcagg cggcaagcat tgccacaacc gtgcccataa gaaggtactt
    40441 tacccaacgt cctttcattg ttctcttctt caggttaaaa aataaggcgc aacgaagata
    40501 acaaaccgcc gcgtttaatg aaatgagcag cctctccctg acgcaaattt tgcacaaaaa
    40561 ataggcttta gtgatttgtt tttgttcaaa atcatgccag atccgtgatc ggggtaaaaa
    40621 aaaggttgca tgaaaacgcg agcggagtat agtgcgcatc cacggaggcg gggtggagca
    40681 gcctggtagc tcgtcgggct cataacccga aggtcgtcgg ttcaaatccg gcccccgcaa
    40741 ccaattattg aacaccctaa cgggtgtttt tttgtttcta ctctcccata aaaaaagcgc
    40801 cattcagcgc ctttttatca tccccttctc gccagcgttg ccccatcggc aaaatacgct
    40861 ttaatccccg caagaataga ctccgcaact tcctgctgga aagtcgccgt tttcagttta
    40921 cgctcttcct caacgttact gataaacgcc gtttcgacca gaatggaggg aatatctggt
    40981 gcctttagta cggcaaaccc ggcctgttca acttgatttt tatgcagctt gttgatttta
    41041 ccgagcttat tcagcactgc tttaccaaac ttaaggctgt cagcaatggt cagcgactgc
    41101 accatatcga acatggtgtg gtcgacatag cggtcaccgc ttttgctcac gccaccaatc
    41161 aagtccgagg cgttctgggt ttgtgccaga tattttgccg cagtactggt tgcacctttg
    41221 gttgagagcg caaacaccga ggaaccgctc ggctggcgac tggtaaaggc gtcggcatgg
    41281 atagagacaa acaggtcagc acgctgtttc tgggcttttg ctacgcgcac ttgcaacgga
    41341 atgaagatgt cttcattgcg cgtcatgtac accttcatat tgccctcttt ctcgatcaga
    41401 gagcgcaaac ggcgagctat ttgcaatacc acatcttttt cgcgcgtttt gtatttcccc
    41461 accgcaccgg agtcttcgcc accgtggcca gggtcaagca taatgacaat cggacgatcg
    41521 cgccctgctt tacctggttg tggaccactt tgcgctggcg gcacctgctt ttcgaggtcg
    41581 cctttgttgt aatcctccag cagcgccagc agcgggtcct gcatatcctg tgcattggcc
    41641 ggatagaggt ccatcaccag acgctcttta aacccggcga ccggcgcaag ggcaaacagc
    41701 tgcggtttta cgttttgctt taattcaaaa accatccgta cggtttgcgg gtcaaattgc
    41761 ccgacgcgcg ccgacttgat gaacgggtcg tctgcacgaa tttgtgccgc catccccttg
    41821 agtaccgagt tcaggtttac atcttcgata tccaccacca cgcgttcagg gttactcaac
    41881 gcgaactgct tatatttcag ctgacgattt gattctaccg tcacgcgggt gtagctggac
    41941 gcaggccaga cgcgcaccgc cacgacctgg ctaaccgcag ccaggctgac ctgacttacg
    42001 ctcaatagcc acatagcgcc cgcgccttgc agtaaacgac gacggctgat tgcagtgttg
    42061 gatcctgaca tgcctctccc gagcaaaaga aatctaagct gtgtaacaag taaacgacta
    42121 atttgaccgg aaactttagc gaaagacgca taatctgtca tctaataaac ggtaaacatt
    42181 ctttttatat tcacggcatt actgataaaa aagtcgctct cgcataaaat ttacacttgc
    42241 accctgcgaa aaaacagaat aaaaatacaa taatttcgaa taatcatgca aaggggtgtg
    42301 ccgtggtaaa ggaacgtaaa accgagctgg tcgagggatt ccgccattcg gttccctata
    42361 tcaataccca ccggggaaaa acgtttgtca tcatgctcgg cggtgaagcc attgagcatg
    42421 agaatttctc cagtatcgtt aatgatatcg ggttgttgca cagcctcggc atccgtctgg
    42481 tggtggtcta tggcgcacgt ccgcagatcg acgcaaatct ggctgcgcat caccacgaac
    42541 cgctgtatca caagaatata cgtgtgaccg acgccaaaac actggaactg gtgaagcagg
    42601 ctgcgggaac attgcaactg gatattactg ctcgcctgtc gatgagtctc aataacacgc
    42661 cgctgcaggg cgcgcatatc aacgtcgtca gtggcaattt tattattgcc cagccgctgg
    42721 gcgtcgatga cggcgtggat tactgccata gcgggcgtat ccggcggatt gatgaagacg
    42781 cgatccgtcg tcaactggac agcggtgcaa tagtgctaat ggggccggtc gctgtttcag
    42841 tcactggcga gagctttaat ctgacctcgg aagagattgc cactcaactg gccatcaaac
    42901 tgaaagctga gaagatgatt ggtttttgct cttcacaggg cgtcactaat gacgacggtg
    42961 atattgtctc agaacttttc cctaacgaag cgcaagcgcg ggtagaagcc caggaagaga
    43021 aaggcgatta caactccggt acggtgcgct ttttgcgtgg cgcagtgaaa gcctgccgca
    43081 gcggcgtgcg tcgctgtcat ttaatcagtt atcaggaaga tggcgcgctg ttgcaagagt
    43141 tgttctcacg cgacggtatc ggtacgcaga ttgtgatgga aagcgccgag cagattcgtc
    43201 gcgcaacaat caacgatatt ggcggtattc tggagttgat tcgcccgctg gagcagcaag
    43261 gtattctggt gcgccgttct cgcgagcagc tggagatgga aatcgacaaa ttcaccatta
    43321 ttcagctcga taacacgact attgcctgcg ccgcgctcta tccgttcccg gaagagaaga
    43381 ttggggaaat ggcctgtgtg gcagttcacc cggattaccg cagttcatca cggggcgagg
    43441 ttctgctgga acgcattgcc gctcaggcga agcagagcgg cttaagcaaa ttgtttgtgc
    43501 tgaccacgcg cagtattcac tggttccagg aacgtggatt taccccggtg gatattgatt
    43561 tactgcccga gagcaaaaag cagttgtaca actaccagcg taaatccaaa gtgttgatgg
    43621 cggatttagg gtaagcgatg aaaatcgccg gatgcggcag gcgtaacact cgtgcgtcgc
    43681 atccggcaat tacgtttatc cccgcgaact aaacaacgcc gccagaccac tgcgccgctc
    43741 agtacgagtg gcgattgccg cacttaatat acgctcatcg gcatacagcg acagacggcg
    43801 acgcgcgcgg gtcaccgcgg tgtataccag ctctcgcgtt actatcggcg tgcgttggct
    43861 cggtaaaatc aacgccgcat ggtcgaactc cgatccctgc gatttatgta ccgtcatcgc
    43921 ccacgtagtt tcgtgctctg gcaagcgact cggttgcaca gacttaatat tgccgtccgg
    43981 catcgcaaac cagacgcgcg tcccctgccc gcgatccagc gcaataccga tatcgccatt
    44041 aaacaaccca agcgcactgt cattacgggc aatcatcacc ggtcgacctt cgtaccaacg
    44101 agagtgcgaa tgacgatgaa ttttgcgctt ctgttgcata aactgctcaa ttcgctcatt
    44161 cagtccagcc acgccaaatg gcccttcccg cagggcgcac aaaagttggt actcattgaa
    44221 cgcctgaatg attaaatccg gctcggcacg cgcttgcagc agatccagat aacgtccata
    44281 acccgcaaga gcttcctcaa gcatcgcaat ataatcttcg ccgctctgta aaagccgttt
    44341 ttcaatatca gtaaaatcct gctgaaaaac ggttttcact gccgttttat caccacggtt
    44401 gatcgccgca gctaactgac caatgccaga atcgctgccg aaacgatagc ttttttgcag
    44461 caggcagaga ctgtcgcgca aagatgccgc ttctgtgccg gttcctgccg gaacgtgagt
    44521 tcccgtcagg cggcttagct gcccggcacg ctcggcggta aagccggcat tggcataagc
    44581 gcagatatcg cccagcacag ccccagcctc aaccgaggcc agttgatcac gatcgccgag
    44641 aaagatcact cgcgcatgat cgggcaaggc gtcgatcagt ctcgacatca taggcagatc
    44701 gatcattgac gcttcatcta ccaccagcac atcaagatgc agcgggttac cggcatgatg
    44761 acgtaaacgc tggctacccg gctgcgcgcc cagcaatcgg tgcaaagtgc tggcatcttc
    44821 cggaatgcgt ttcttttgtt catcggtcag cggtaactgt cgcaaagcct tgccgagaga
    44881 ttcggttaag cgcgcggcag ctttacccgt tggtgcagcc agacggatac ggcagcgttc
    44941 gccgtcggcc atttgaatta acgctgccag caacttcgcc acggtggtcg ttttaccggt
    45001 gcctgggccg ccggaaatca ccgagatccg ccgcgtcagc gccactgctg ccgccacttt
    45061 ttgccagtta atttcatcgc ttactggaaa aagtttgtcc agggtttgcg ccagtagagc
    45121 ttcatcaacc tcaatggcat gattcacctc attgaagaag cgcgctaccg tccgctcgtt
    45181 acaccacatg cgattcaaat aaagacgatc gccacagagg atcatcggcg tgggttcatc
    45241 tcccctgctg accgcttgag aagccagcaa gcattcttcc caattttgta gctcaccgat
    45301 ttcactgaca caggtcgcca acagcggatg cgacgcctcg ttattttcca gtcgtgaaag
    45361 cggcaaacag acgtgtccct ctccggcatc atgacttaac agtgccgccg cgagggtgac
    45421 ggcaggatgt tcatctcccg ccacggtcag ggcaaattgc acatccagcg ggcgtagctg
    45481 tttgtgctcc acagcttcca gtaattgctt ttgcaatttc attacgcctc ctccagggtc
    45541 ataccggcaa acatctcatc catcagggca atcaacccgg cgttgggtcg ggttgtgtaa
    45601 atcccttgtt gcggatgttc tttatcaacg ccacgcagga acagatagat aacgccgcca
    45661 aagtgacgat catagtcgta atcagcaatg cgatggcgca gataacgatg cagcgccagg
    45721 gtataaagct gatattgcag atcatagcgg tgtgcctgca tcgctgctgc catagcctgt
    45781 tgggtgtaag ccgaactgtc ttcacccagc cagttggatt tatagtcgag caggtaataa
    45841 cgcccttcgt ggcggaatac caggtcgata aagcctttta acatgccgcg tacctgcatg
    45901 aactccagcg gcgggcagcc agcggatagc gggtcaaact ggcgaattag tgcatcaagc
    45961 tggctggcga tgagcggttc gctaatcggc aggtaaaact ccatctccac ctgtttatcg
    46021 cggtcggaaa gctgactcag gctaacaccc gtttcattga gaggtgcctg gaggacagcc
    46081 gtgatccact cggtcaacac cggttcccac tgcgattcaa agccgccgag ttccagtttt
    46141 tcctgtaccc agttcgggtc aaccggctgg gtaaaatcga ggtcttcaaa caaactgtgc
    46201 aagaatgtcc ccggtgacgc accgcgcggg aactgatgcg gtgttaacgt cggttcttca
    46261 acgacgctga ccacgcctgc ggcatcaaca tccagccgtg gcatcagatc ctgagcgata
    46321 ccgtgaccac gctgctgcaa gccggagtag ctggtgacac gccagttatc gccgggcaat
    46381 cgttgtagtg tcctcgcatt cagttctgca gttaaagcat cattaacctg ccagggctgg
    46441 ttatcaccag tttgtgccgt ttgccaggca atatcatcat cgcataacgc ttcaatacag
    46501 gtgcgaagcc ctgccgcatc ttgcggttcc cctttttgca gcaaacgccc gagcgcactt
    46561 tggtggacgt cggtgtcacc ttttttatcg ccacgacggc gcaccagcgg tgcaacgccg
    46621 agactgcaat gccaaaccga acgcgtcagc gccacgtaaa gcaaacgcag atcttccgcc
    46681 agacgttcga cctccgcgag gtcgacgctt tctggcgcag cattaagatc caaaactgcc
    46741 tcaaacgagt ggcgatcgtg ataaaacgcc tgatcctgga cgcggaaatt ggtaataaat
    46801 ggcaaccaga ccagcggata ttccagccct ttcgatttat ggatcgtgac aatctgcacc
    46861 agatgtttat cactttcgag gcgcatttgt tggctggagg cattactgtc tggctcgagg
    46921 atatgttgcg ataaccagcg taccagcgca tgttcacttt ccagctgcgt tccggcttct
    46981 tgaagcagtt cgctgatatg caagatatcg gtaagacgcc gctcaccgcc tgccgttgcc
    47041 agcaagtttt ctgcaatgtt acgcgccgac atcagcgccc gcagcatcgg cataacgcca
    47101 cgtttgcgcc agatttgccg ataaccatcg aactcttcga ctaccgcatc ccacgcatgt
    47161 tcgtcattat tcagcgtttc aatatccagc gcattcagcc ccatcattga cgttgccagc
    47221 gcactgcgca gggtgttctc acgttcgggc gtcatcaccg cctgcaacag ccaaagcatt
    47281 tcctgcgctt ccagagtttc aaaaacacta tcgcggttcg aaaggtaaac ggaagggatt
    47341 tccagcaacg ttaaggcatc gcgcacctgg gcggcctcct ggcggctgcg caccagcacg
    47401 ctgatgtccg aagcacgcac cggacgcgcg tcgtcgccgt tcatcagcaa cgcttcgccc
    47461 cgctgtccgg cttgtagcca gtcgcggatt tgcgcagcac atacctgcgc catggtactt
    47521 tgatagtcgc caacgccgca gctttcgcct tccatcagcc acattttcat cgcaggctgc
    47581 gtttcacctt taaagacaaa acgtaacgcc tgatttttcc cggctgattt cactggaata
    47641 aacggtattt cgcgaaacat gaacgcgtca tcagtctggc tgaaaagctt gttcacactg
    47701 ttcaccattc caggtgcgga gcgccagttg gtgtctaaag tgtagtgggc gtgaacttca
    47761 ctacgcgcct tcatataagt gaagatatcc gcaccccgga atgcatatat ggcctgcttc
    47821 gggtcgccaa tcagcaacaa tgcggtttct ggctgatggt gccagatacg gcgaaaaatt
    47881 cggtactgct gggggtcggt atcctgaaat tcatcgatca ttgccaccgg gaatcgcgta
    47941 cggatcgccg ctgccaacac ctcgccgctt tcgctacgca gcgcggaatc gagccgactt
    48001 aacatgtcat caaaacccaa ttcgccacgg cggcgttttt cacgcgctac tgtttcgcgg
    48061 atctcagcca atgcgcgggt gatcaccaga tcacggatcg acaatggttc tgcaagcagt
    48121 tgatcgatcg cctcaaacag tggatgtcgc ggggtttccc ccccggcttt cgtgcgatct
    48181 tctaagaaac gctgggagaa tttttccagc gactccggca actgataact gtttgtctct
    48241 tcttctgccc aggcgctgat cttgtcgatc catttagcct gattgctacg gttaaacttg
    48301 cgtcgatcaa taccagaaga ttcgatcagc gcatccagtt cacccactgc gtcgcgccac
    48361 tgctgtttta ccgtatcaat acgcgccaca atttgcgcgt gacgggaagc tagcgtttca
    48421 tcatcgggcg gcggtgcttt gataaccggc gcttcgcctt gcagataacg attaatatcg
    48481 cgcagcaacg cctgcggccc tttccaggtt tcaaagacga cctgggctat ttcacgcggc
    48541 agcgggtagc agtggcgacg ccagaaatcg gcgcaggcct ggtagcgtag cagagactca
    48601 tcttcaatca gctgctgctc aaacagcatg ccggattcaa aggcattcag gttgagcatg
    48661 cgctggcaaa agccgtgaat agtaaagact gccgcttcat ccatctgccg ttcggctaac
    48721 aacaaccact gcgcggcttg cgctttatcg tcgatctctt ccagcaggtg ttcgtacaat
    48781 ggattgtcgg tggtttcacg cagacaggcg atgcgcaact cgtggatatt gctacggata
    48841 cgaccgcgca attctgccgt ggcagcctcg gtaaaggtga ccaccagcag ttcttcaacg
    48901 gtcagcgggc ggggaaaggc ggcggaaccg cctagtccaa gtaacaggcg caaataaagc
    48961 gccgcaatcg taaaggtttt gcctgtgccg gcagaggctt caatcaggcg ctcgccctgt
    49021 aagggcaagc gcaaaggatc tagtgtctcg gcgacatcac tcattctttt cactcatcag
    49081 gggcattgtt tgctgcaacg cgctgacgtt ctcccacact ttccagcctt caggatgcac
    49141 atattcggct ttcccgtttt ggctgccgga aatctgcgac aggatagcca tgccttgcgg
    49201 ctcgactacc gcctgatgga agaaatcggc cagtttttgc ggcgtcagca gtttaatctg
    49261 ggccacgatt ttatcacgcg aatcgaagcg catattgccg cgatcgaaat ctttacttaa
    49321 cttcgatgct tcttcgccga gcgtttgcgg tgcctgcagc atctgggtaa ttaccgcctg
    49381 ctggatttgc gcaaactcat ctggcttcat cgctcgcaat tttgcctctg cggttgggaa
    49441 aaacgccttg taacgctccc acaagaatga aggctgttta tcattgcttt gcaaaaggaa
    49501 gcccatgccc cactgacgcc ccacgctcat tggaaacgca aacacggcat agcccaattg
    49561 ttcttcggta cgcaactgat tgtagaacca cggctgtacg atctgcccca acagagagct
    49621 ataggctgag ctggtgtatt catcgtagcc agtcggtaca aataccgctg ccagtgcgga
    49681 gtcggtgctg ttaccggctt tttcaaagat gacggattgt tttttatcga ccactacatc
    49741 tttgtttcga caccactctg aaccatcagc gcccaactgt ttttgcacat cgcgtgccag
    49801 cgttgttgcc tgggcctcgg tcatgttgcc gataaccata aactctggtc gagcccctga
    49861 ttttaaggcg tcgcgataag ccagcacctc tttcaacgta atggagggca aaattttacg
    49921 ccgttcatct cgcgagaagt acggcacttg cgagagcatc tgcgcgggca taatcgcctg
    49981 ctcaaacgct ttaccctttt ctgcggaatc catcatctgg ttataccagg acttcgcctg
    50041 ctcaagctga tcttccgtag cggtatagct aaagtacccc tcgagcaatg cctggaacag
    50101 ctgcggcaga cgctgggggt aaccattagc attaaccata aggccgttgt tagcgttggt
    50161 ggaaaaactt atgccaccaa ccgacgcctg gttgcttaac tgatcaagcg ccagccctgc
    50221 gagataatca ttgagcgcaa acatcacctg attgcgggcg ctgtccatgg ctttcggatt
    50281 acgcaaaatc aggctgacat cagctttggg ttcgctggaa aaataacggc ttggcgcata
    50341 caccacgcgc agattcgact catcaacaat cagctctgga tggtcatatt tcttctctga
    50401 cttaatcagc gagaaatcgt caggaatata ggggttaagc tccggtaagg agagcgcaat
    50461 attggcagct ttttgctgcc agtcagcgaa agtttgttcg ctgattttat cgacctgata
    50521 cggcgcatcg acaaaataag ccgttttatt gtgcggctct ttcgggctga tataccagat
    50581 acgcgcattc tgcggcgtca tcatcgccag acgttctttt actgctttag catcgtaccg
    50641 atcggcaata ttgactgcat ccagcgtatg ctcaaccgga acgcgaatca tggtatctgc
    50701 cagccattcg acgtaatcca tatcacgggt gatcgacgga taacggaagt cgatatccag
    50761 cacattcgcc agttcatcga agtattgttt atcaatgcct ttttcacgta acagattgag
    50821 atagctaaaa attgccgcca caacctgatc gcgattagcc aggcctttat cggttaaaga
    50881 cgcagagatc gctaatacgc cgctgttgcc gttgacgata ggatcggagt tggcgctaat
    50941 gccctcaact aatccctgct tttgcagcca gtcggaaagt gtacctgggc tgcgattacc
    51001 aatcagatag gtaatcaatt catccgtttt actacggaac ttcgctgagt tgttatcgat
    51061 gcgaaactca acgcgcagaa ctttacgcgg cattgccggg acgtaatgaa tgataatgcc
    51121 cttttgcgcg tcggtgacta ccggcacggt gatttccggt tttttgctct ctttgttcgg
    51181 cacgcgacca aaggtgtccg ccgccatttt tgccaactcc ggcagcggtt tattgctgta
    51241 aataaccgcc ttcatcagat tggcggaata gtacttctcg tggaaatctt tcagcgcctg
    51301 ctgcaccgga ttaccaggtt tatcgcttaa agtttcgagg ttaccaccag aaaactttga
    51361 accggggtgt gccgggttaa tggtttctgc gctgacctgt gccatgcgca tcccgtcacg
    51421 cgtacgcgcc atggttaatt cagcgttcac cgcattacgc tcacgttcgg catatttctt
    51481 gtcgagcaaa ggttcagcaa tagcatcggc caggcggtct accgcaccag gcaaggcgtc
    51541 gttctcaact tccagataga aagccgtgcg atacggcgca gtgctggcat tgtgactacc
    51601 gccgtgcatt ttgagatatt cggccagact gtcagcctgc gggtactttt tcgaccccat
    51661 cagactcata tgttcaaggt aatgtgccag cccctggtac gcctcgggat cttccagcga
    51721 cccaacgggc accaccagcg ccgagagcga tttaactgcc tgcggatcag aaaccagcaa
    51781 gaccaccata ccgttatcca gacgtatagc ctgatactgg cggttatctt tatcactttt
    51841 acggatggtt tcctgaatcg gctgccatcc cgtttctgcc tgacttaagg gtgcccaaag
    51901 ggcaactaac aacaataatg ctttgaacca ggtgctgcgg ggcattctcg gacctcataa
    51961 tcaacttaat tttctgtcca gattcaacac gttaacgcaa atcatctgcc agaatttaat
    52021 cttgtgctgc acgagtcagc ctatgtttat ataaccatca gtccgtgact ggtgcgcatc
    52081 ataaagtaag cggatagatt gcgcaatttt tatacagcac tcatgactga ttaaagcgaa
    52141 acagcggtaa caggaaacgt tgcgactgtt caacgatggc ctccattgtc tctggtgtta
    52201 attgccgcca gagcctttga taccagatat catcaccttc gccacgcacc atcatgttgc
    52261 cttcgtaagc ctgaaggaat ttcgtacggg ctttttgcaa cgtggaatcg tcatccagca
    52321 tggcatcgtt ttgcgcgtca taacaggttt ttagccacgc gccgccactt tcaggtaaca
    52381 ccagcaatgg cgcggacatc ccttcacgat acccctcaat cagttgtgag aggtaatgca
    52441 aagcctgttc ggctgcaagc ggcggaaaac gccactcgcc gtctttgcgt agaaaaaggc
    52501 gactttcacc attaccaccg ctggcacagt agacaaggtg ttccagccaa agttgcattc
    52561 cctgcgccac acttaataaa gagggacgcc agcgcaacag gccatccggc tgcacctgcg
    52621 gcaaccagcc agttatctgc acaccgttgc aggcgagatc aatttccata ctctgccccg
    52681 gctggcgaca ggcaatgact ctgtcggcaa gctgctgcat ctcctggcac tgagtttccc
    52741 agaaaatttc accaaaagcg ccatacggta aatcccctgc cgcccggaag cgacggaaca
    52801 agcgttcggc atcatcctgc tcaaccagtg cattcaataa ctgctgattg atttgataac
    52861 ggctaagccc ttccagaata aatggctcgg tgtcggggat ttcgctgtct tcagtacgga
    52921 agttcacctg caaacgcatc tggaagaacg cccgcaccgg atgtgcccag aatcgttgta
    52981 gcgtttccag cggcacggtt tccggtaagg taaacggcag cggctgaaca aattcagaat
    53041 gtgctttacc agcctggctg gccgcaggta gccattcacg agcatagctt tgtcgttcgc
    53101 ctggctgata gttttgtgga tcaaacggca ttcgggtatg gaggcaagta agatgcgctt
    53161 ttacccttgc ctcgctttca tcacagttga gcgcttcatc gcccggtaga taatgacttt
    53221 gcccgatgta gtcgatcagt tcctgcacca gtaccgacgg gaaacgttca ctgttatcct
    53281 gaatggaacg cccgatatag ctgatataga gtttttgctg cgcggaaatt aacgcttcca
    53341 ggaacagata gcggtcgtca tcgcgacggc tacggtcgcc acgcttcggt ttctggctca
    53401 tcaggtcaaa gcccaatggc gcaagctgac gtggataaac gccgtcgttc attcccagca
    53461 ggcaaaccac tttgaacgga attgaacgca ttggcatcag agtacaaatg ttaaccggtc
    53521 cggcgagaaa acgctggctg atacgttctt gatccagacg ctgtgccagt tcatcacgca
    53581 atagtgacag cggcaccgcg tcgccatact gcgcacctaa accttcggcg ataatcgcct
    53641 gccattgttg ttcgatcagc gtcatcgccg cttcggtttc cgcatccggc aggaagaagg
    53701 cgctgagcat atcgcgacaa accggcaacc actcttctag cggacgctcc tgtgccagcc
    53761 cgcgacgcca gatgtttagc tgcattagca gtgaagccag atgccccacc agttctgcaa
    53821 ttaagccgct cgattcatca taaggtagaa ccgattgcca ctcgccctgc gcgctctcca
    53881 tcgcgtagcc cagcaacata cgcgtcaggc caaatcgcca ggtgtgttgt ccggtggcgg
    53941 ggagttccag ctcgcgaacg ttgtcgtcat ctatccccca acgaatgccg gattcgttga
    54001 cccactggcg taaataacgc agcccttctt cggtgatgtc aaaccgcgcc gccagcaccg
    54061 gcacatccag taatgccagc acgtcttccg acacaaagcg gctgtctggc aacgataaca
    54121 ggctgataaa cgcttccagt accggatgcg actgccgcgc ccgacggtcg gaaatggcgt
    54181 aaggcaggta acgatccgca ggtgcgctac caaacacagc ccgaataaac ggactgtagc
    54241 tgtcgatatc agccaccatc acgatgatgt cgcgcggagt aagtgtcggg tcttcttcca
    54301 gcatcgccag caggcgatcg tgcaaaactt caacttcacg ctgcgggcta tggcaaacgt
    54361 ggaaggtgat actgctatcc agtggatcaa gcgggcgttt gttatcgcta cgggaaaact
    54421 cttcgatgtt cacaccagca acggcacggt tttccagttc cagaatgtca gactgaatgt
    54481 tatgcagcag gttatctggc gtgacatcga caaaggcgtc cagctcctgg ctgctctcca
    54541 ggtcagaaag gagataaatg tagtcgcgcc caagcttacc ccatgaagcc agcagcgggt
    54601 tgccgacatc ctgttcacca tcgctgttaa agagctgccc ggcattttcg ctatcacgaa
    54661 atagcggcaa ttcgcgatct tcaaaactgt gtcgacgctg acgggtcagc agtttcgcca
    54721 gataagcagg atctttaata tcgccccagt aataacggca ggggttggta aacaggagat
    54781 ggatttcaat atgtttaccc agcgcctgta gcgcctggag ataaacaggc ggtaacgcgg
    54841 aaataccgca tataaagacg cgcgaaggta accccggcgg gcaggtcgtc gcggactcca
    54901 gcgtttcgat aaagcgctga tagagattgg cgcggtgcca gcgcggttgc ccgagttgat
    54961 gggtatattc caccagcgcc ttccacaacg gggcttgcca ggcctgtgct tctcccagcc
    55021 cttcaaccaa atgtcctgtt tcccactgtg ccagccagtc cggacgatag accagatact
    55081 ggtcaaacag gtccgccgct tttgaggaaa gctggaacag ttttcgcttg tcgctatcgt
    55141 cagtcagata atgccgcaac agggtaaagt cttcgcgctc cagcaattgc ggcagcagag
    55201 tcatcagttt ccagctcatg ctctgtttgt taaaggcgct ctctttgggg atttccggta
    55261 acacccggac gaacatatcc cagataaagc tcgctggcag cggaaaatca atgtttgccg
    55321 caatactaaa cttttgcgac agggtcattt gcagccactg cgccataccg gtactttgca
    55381 ccagaatcat ctctggttcg aaaggatcgt ccagccgttc gcgttcgaca ataaactcca
    55441 tcaacgcttc cagcacgtcc agacgattgg aatggtagac ccttaacata gcggctcctg
    55501 actactgacg attcgggcaa tgcaggcggg tcatctcgcc ttctctgccc ccgggtgaaa
    55561 ctagcgtaac gctgatgctg acacatcccg cctgcgatgt ctgcattcgg ttgacctgcc
    55621 agttggcagg tggcgaaatc gcgcgcagtt gcgtttgctg ccaaccatgc cgccagagct
    55681 gttggtactg gtttctgctg gcgagactgt tcattaatgt tcgctggata cccgataacg
    55741 cagtgacaat catcaccatc agcaccatcg caaccattac ctccggcagg ctaaagcctt
    55801 gttgattctt cagggaagct gacataacgc cctctctttc agcggacaaa aatcgctcca
    55861 gccgcgtggc gaaaaaacaa tgtttccatc aatgacttcg cctgttcgcc acaacgaaac
    55921 gccttcataa ccggcaacca ataaggcttc attatctgcc agtaaacgca aacaaacccg
    55981 ggcatcggtt ccagcgtact gcgagcactg aactgcgggc tgcgtctgcc aggagtgcat
    56041 tttcccccac gccagcgccg actgaacgat ggcctggcgg cgcaatgact gactttctat
    56101 gctcacgcga gaggcaaaac tgcgatcctg ctgactcatt ccttgcaata gcaagctacc
    56161 caaaaccagc agcatcagga ccagagccag tgacgaaaca cctttttcgc ggttcacagg
    56221 ttgaatcctg tcacgctata gctggcatcc accacggttt gcggttcaga cttactggca
    56281 gcacgcatat taaccgtcaa caccggcgag aagccgctga catcctgacg tacgacctga
    56341 aaagtgtcga taatgatggc atccggatta gtgactttat cccagccctt accttcacag
    56401 gatgtcgcac cgcgtagcgt ttccagcaca tgctccttca gacgaaatcc aatctggtca
    56461 gactctttta ccggttcgcg atcccagata ccgttactgt tcgcatccca ctgcacaatg
    56521 atacagtcac cctgtccgac aatttccagc ccttcgccgg tacagctgcc atgacaataa
    56581 cccgccctct ggagatgctt cgcgacggta aatacccgca gccagatttc atcttccagc
    56641 gccagcttac gggtgctcgt taaactttca cgctgtaacg caggcagaaa gcgtgcagcc
    56701 cccagcaaca atacgctact gatcgccata gcaatcaaca cttccagcag agaaaaacct
    56761 tgctctttta caggcatcct tctgtttctc cttgctgaca aagccggagt cttccccacg
    56821 gcgaaaccac cagccaccac tcgcccgttg agtttttgaa gcgaatatgc ccggcccatg
    56881 cggtattgcg caggccaaag aaagcaagcg aaggtgtcag gtcgctcatt tagacttcgg
    56941 gccagcgtgg cacaaagacc aatggtgaac tgccatgaca ggtattggcc ccagcaacgg
    57001 aactcacaag gcaccataac gtcccctccc tgataacgct gatactgtgg tcgcggttat
    57061 gccagttggc atcttcacgt aaatagagca aatagtcccg cgcctggctg gcggtttgcc
    57121 atagccgttg cgactgctgc cagtattgcc agccatagag tccacttgcg cttagcatga
    57181 ccaaaatcag catcgcgacc agcgtttcaa tcagcgtata accacgttgt gttttcatgc
    57241 cggcagtatg gagcgaggag aaaaaaagac gagggccagt ttctatttct tcggcgcatc
    57301 ttccggacta tttacgccgt tgcaggacgt tgcaaaattt cgggaaggcg tctcgaagaa
    57361 tttaacggag ggtaaaaaaa accgacgcac actggcgtcg gctctggcag gatgtttcgt
    57421 aattagatag ccaccggcgc tttaatgccc ggatgtggat cgtagccttc aatctcaaag
    57481 tcttcgaaac ggtagtcgaa gatggattcg ggtttacgtt tgataatcaa cttcggcagc
    57541 ggacgcggtt cgcggcttaa ttgcagatga gtttgatcca tatgattgct gtacaggtgc
    57601 gtatcgccac cggtccagac aaaatcaccc acttccagat cgcactgctg cgccatcata
    57661 tgcaccagta acgcgtagct ggcaatgttg aacggcaggc cgaggaagac gtcacaggag
    57721 cgctgataaa gctggcaaga gagtttgccg tctgccacat agaactggaa gaatgcatgg
    57781 cacggtgcca gcgccatttt atccagttcg cctacgttcc acgctgaaac aataatgcgg
    57841 cgggaatccg ggtcgttttt cagctggttc agtaccgtag tgatctggtc aatatgacga
    57901 ccatctggcg ttggccaggc gcgccactgt ttaccataca ctggtccgag gtcgccgttt
    57961 tcatcggccc attcgtccca gatggtgaca ttgttttcgt gtagataagc aatgttagtg
    58021 tcgccctgca gaaaccacag cagttcgtgg atgatggaac gcaggtggca acgtttagtt
    58081 gtcaccagcg ggaatccatc ctgcaggtta aaacgcatct gatgaccaaa aatggaaagc
    58141 gttccggttc cggtacggtc gtttttctgt gtgccttcgt cgagcacttt ttgcatcagt
    58201 tctaaatact gtttcatggt tcctcaggaa acgtgttgct gtgggctgcg acgatatgcc
    58261 cagaccatca tgatcacacc cgcgacaatc atcgggatgg aaagaatttg ccccatgctg
    58321 atgtactgca cccaggcacc ggtaaactgc gcgtcgggct ggcggaaaaa ctcaacaatg
    58381 atgcgaaacg cgccgtagcc aatcaggaac aagccagaga cagctcccat tgggcgcggt
    58441 ttacgaatat acaggttgag gataataaac agcaccacac cttccagcag cagctcgtaa
    58501 agctgtgatg ggtggcgcgg cagcacaccg taagtgtcga aaatggattg ccactgcggg
    58561 ttggtttgca gcagcaaaat atcttctgta cgggagcctg ggaacagcat ggcaaacggg
    58621 aagttcgggt caacgcggcc ccacaattca ccgttaataa agttgcccag acgcccggca
    58681 ccaagaccaa acggaatgag tggtgcgata aaatcagaaa cctggaagaa ggaacgttta
    58741 gtacggcggg caaagataat catcaccacg ataacgccaa tcaggccgcc gtggaaagac
    58801 atgccgccgt cccagacacg gaacagatac agcggatcgg ccataaactg cgggaaattg
    58861 tagaacagaa cataaccaat acgtcccccg aggaagacgc cgaggaagcc cgcatagagt
    58921 aagttttcaa cttcattttt ggtccagccg ctgcccggac gattcgcccg tcgtgttgcc
    58981 agccacattg caaaaatgaa acccaccaga tacatcaggc cgtaccagtg aagcgccacg
    59041 ggtcctattg agaaaatgac cggatcaaac tccggaaaat gcagatagct actggtcatc
    59101 tgtcaccaca agttcttgtt atttcgctga aagagaacag cgattgaaat gcgcgccgca
    59161 ggtttcaggc gctccaaagg tgcgaataat agcacaaggg gacctggctg gttgccggat
    59221 accgttaaaa gatatgtata tgatccgcgc tataaccctc cgcgaatcag cccgcccatg
    59281 ccgcgacgct ccataaaggc tgcaacctga tggcgaactt cggtcgccag ttgcgcttcc
    59341 agactacgct gcgcaagatt ttctgcttcg gcataatcaa tgcgccgcag caggtatttt
    59401 acccgcgcta cagaacgtcc gttcatagac agatggcgat acccaagccc aatgaggatt
    59461 gccacgcaca tgggatcgcc cgccatttca ccgcacaaac ggagatcgat tccatgtatt
    59521 tccgcttccc gggcgatcat cgccagagct cgtaacattg caggatgaag actgtcataa
    59581 atgttcgcca cccgggtatt gttgcgatca acggccagaa tgtattgagt cagatcgttg
    59641 gtgccaacag agatgaaatc gacccgcttt gccagatgcg gcagcataaa taccattgac
    59701 ggcacttcca gcatgatgcc gatacgtggt ttgggaattt cgtaaccgat catctcctcg
    59761 acttcacgtc cggcacgttc aatcaggcgg cgtgcttcat caacttcatc gaggcttgtg
    59821 accatcggca acagaatatt caggttgccc gtagcggcat tagcacgcag catcgcccgc
    59881 acctggatca agaagatctc cggctgatcg agcgtaatgc gaatcccacg ccaacccagg
    59941 catggattct cttcgctgat cggcatgtaa ggcagctgct tatctgctcc gacatccagc
    60001 gtacgcaagg tgacgggttt atcattgaac atttgcagca tcccctgata ctgcgccacc
    60061 tgttcttctt ccgacgggaa accactttgc agcatgaatg ggatttcagt gcgataaaga
    60121 ccgatgccat caatacggct gcccagtttt tcttcatgtt ccgggcttaa accagcattg
    60181 agcatgactt ttatacgctc accgcttttt aactgcgcgg gtaaattgac gtcatcttcc
    60241 gccagacggc taagctcaat ctcttcacta attagccgct gatattcttg cagcagtacc
    60301 ggctccggat cgaccagcaa ttcaccgcga tagccatcaa cgatcagcgt cctacgatgc
    60361 agcaccgaag gctgaatatc cgcgcccatc acggtaggga tccccagcgc acgtaccatg
    60421 atcgcagcat gggagttggc tgcgccatct cgcacgacaa caccgactaa gcgatcctgg
    60481 ggcagctcag caagagttgt cgctgacagt tcatctgcca ccagaatgaa acgttccggc
    60541 caggcgttcg gcccttgatt agcgtcatca agatgaaaca gcaatcgctg acccagcgca
    60601 cgtaaatcgc cagcccgctc tttgagatag ttatcgctta gcgcggcaaa ctgttcggca
    60661 aatttttcaa tgaccgtttt taccgcccac tctgccaccg agcctttatc aacctcggca
    60721 aacaattcgc gacgcagccg ggtgtccgaa agtaggtgcg agtaaagatc gaaaatagcc
    60781 gccgtttctt tttgtgctcc ggcggcaaag cgtttgctgt agcggcgaaa ctcgtttgcc
    60841 gcttcttcca gcgccccggt cagtcgttcg cgttccagag ccggatccag cgttgatgcc
    60901 tgatacacct gttccattaa aggtaacgtg gcatcctgcc agccttcggc aatcgccaca
    60961 ccaggtgctg ccggtaatgc gcggattcgc gtctggcgat attgcccaaa caaggcagtc
    61021 aactgcgact gagaaagaat agctgccatc tgggtggcaa gcgtcaccag gaaggattct
    61081 tcactttcgt catactggcg caactctcgt tgctgtacca ccagtacacc aagcaactgg
    61141 cgacgttgaa taattggtac gcctaaaaac gcgcggaaac gttcttcttt tacggagggg
    61201 atgtatttga agctggggtg cttttgcgca tctgcaaggt ttatcggttc cgccagcctg
    61261 ccaaccaggc cgacgatccc ttcatcaaac gcgagcgtta cagtgcgacc gcgtggtttt
    61321 ttcagccccc gggtcgccat caggtagtaa caacgtcgat catgatcggc caggtagacc
    61381 gaacagacct cggtatccat cgcaagacag atgtcggtaa ccagaatatt taacgcctca
    61441 ttcaggcgtg gtgcgctggc taccttttcg actatttcgc gcaggcgagt gagcataatt
    61501 ggcgtgactt aacctctttt acgtcgataa gcagatgtgt tttgtggttt tggcgtattt
    61561 tcctgcagtg acatcaccac actcgcgaac tctttcatta ccctacggta gacatcacgt
    61621 ttaaatgaca ccacctgtct gaccggatac cagtaactta cccatcgcca gccgtcaaac
    61681 tctggtgtac tgctggtttg catattgatt tctgcatcgc cgctcaccag ctgcaagaga
    61741 aaccattttt gtttttggcc gatacaaacc ggcttcgtgt cccaacgcac caaacgtttc
    61801 ggtaatttgt agcgcaacca gttacgcgtt gaagcaagga ttcgaacgtc tttgcggctt
    61861 aatcctactt cttcaaacaa ttcacggtac atcgcctgct ctgcggattc tccggggttg
    61921 attccgcctt gcggaaattg ccaggagtgc tgaccaaatc gccgggccca cattacctgc
    61981 ccctggcgat tacaaatcac gatacctacg tttgggcggt agccatcgtc atcaatcacc
    62041 ggactacctc aaaataaagc tttatatacg aatgattgtt tcatactcca agaagacggt
    62101 aaaccactct ctgcagggca ttacacacta ataacaattg aataactcac agttatgtgc
    62161 agagttataa acagaggaag gggtggatag ccgtttttat tcaccttttc tgtggataga
    62221 gttgtgaaga actacggaat tactacggga aaacccggag cattctgaat aagcccgata
    62281 tagacatttt aaaatattca ttattagtca atgcattaca tgtaatttga gtaatgaaaa
    62341 agagtaattc gtgacccagg tcacacctct catttacggg ttggcgaaag atcaaccaat
    62401 gccgtattta tccacagaat gtgccactaa gttaagcact gaaccactaa aaactggagt
    62461 ttcgtcgcac gtcaaggctg taaatggaaa cagtagtgga ggtttttcac agttatccca
    62521 gctttctgtg gataacatgg tgtaagatcc tgtttatttt cagtgaccag atttggaaaa
    62581 cctattgcag tgttgcgcaa ctcgattacc ggcagcctaa aaagcaatat aaatcagaga
    62641 attgaacaac acatgtggaa aaagttacac tgcgaatatt cggcacataa ttgctgtttg
    62701 ttttttaacc aaggtatcat gacatgtccc aacctcgccc actcctctct cctcccgaaa
    62761 ctgaagaaca gttgttagcg caagcacagc aactttctgg ttatacattg ggagaactgg
    62821 cggcacttgc cgggctggtt acgccagaga atttaaaacg cgataagggc tggattggcg
    62881 tgttactgga gatctggcta ggtgccagcg cagggagtaa acctgagcaa gattttgctg
    62941 ctctgggcgt ggaacttaaa actatccctg tggatagtct tggtcgtccg ctggaaacaa
    63001 cattcgtttg tgttgccccg ttaacgggta atagcggggt gacctgggaa accagccatg
    63061 tgcgccacaa gctcaaacgc gtactgtgga taccggttga aggtgagcgc agcatcccgc
    63121 tggcgcagcg tcgcgtagga tcaccgttgc tgtggagccc gaatgaagag gaagaccgac
    63181 agctacgcga agactgggaa gaattaatgg atatgattgt tctcggtcag gttgagcgga
    63241 tcaccgctcg tcacggggag tatttacaga tacgaccgaa agcagcgaat gcgaaagcgc
    63301 ttaccgaagc cattggtgcc cggggcgaac ggattctgac gctgccacgc ggcttttatt
    63361 tgaagaagaa tttcaccagt gcactactgg cccgtcattt tctgatccag tagccatcgc
    63421 tttgacctgc cgctttccgg gcatataatt accgcttcat ttttttggca gggcttttta
    63481 gatgttattt gcatggataa ccgatcctaa cgcctggctt gcgctcggta cgctgacgct
    63541 gctggagatc gttcttggga tcgacaatat tattttcctt tctctggtgg tggcaaagct
    63601 ccccacagca caacgtgctc atgcgcgccg tctggggttg gcgggagcca tggttatgcg
    63661 tctggcgctg ctggcatcaa tcgcctgggt tacgcgcctg acaaatccgc tttttacaat
    63721 attcagtcag gaaatttccg cccgtgattt gattctgctt ctgggtggct tgttccttat
    63781 ctggaaagcc agcaaggaaa tccacgaatc cattgaaggt gaagaagaag ggctgaaaac
    63841 acgcgtttca tcattcctcg gcgctatcgt gcagattatg ctgctggata ttatcttcag
    63901 cctcgactcg gtgattaccg ctgtgggtct gtcagatcac ctgtttatta tgatggccgc
    63961 cgtggtgatt gccgtaggcg tgatgatgtt cgccgcgcgc tcgattggtg attttgtcga
    64021 acgccatcct tcggtaaaaa tgctggcgct ctctttcctg attctggtcg gctttaccct
    64081 gattctggaa agtttcgaca tccacgtacc gaaaggttac atctacttcg cgatgttctt
    64141 ctctattgcg gttgaaagcc tcaacctgat tcgcaacaaa aagaacccgc tctgatactc
    64201 cgtacgctct cctgcgggag ggcgtactca ctcctgttac cctcacagtt taagattttc
    64261 ctgctttcaa gatatataac gtcggtttat aaacagacta ttatcatagg tgggccaaat
    64321 gaggataacc ggatgaaaaa atgggcagta ataatttctg cagtcggact ggcgtttgct
    64381 gtttccgggt gttccagtga ttacgtcatg gcgaccaaag atggccgtat gattttgacc
    64441 gatggaaaac ctgaaattga tgatgatacc gggctggtga gttatcacga tcagcaaggt
    64501 aacgcgatgc aaattaaccg tgatgatgtt tcgcaaatta ttgaacgtta acaaataagg
    64561 tcagcatccg gctgtaatgc cgatcagtta ag
//
LOCUS       DAZPDU010000004        63101 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZPDU010000004 DAZPDU010000000
VERSION     DAZPDU010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 63101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63101
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.004"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..72
                     /locus_tag="ACHURS_000199"
     CDS             <1..72
                     /locus_tag="ACHURS_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1236035.1"
                     /translation="ARRRCDVLFAMMRDGTFYTPQGS"
     gene            481..930
                     /gene="alaE"
                     /locus_tag="ACHURS_000200"
     CDS             481..930
                     /gene="alaE"
                     /locus_tag="ACHURS_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFR1236036.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSHYQQVKA"
     gene            complement(967..1311)
                     /locus_tag="ACHURS_000201"
     CDS             complement(967..1311)
                     /locus_tag="ACHURS_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFR1236037.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            1463..1792
                     /locus_tag="ACHURS_000202"
     CDS             1463..1792
                     /locus_tag="ACHURS_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFR1236038.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            2040..2285
                     /gene="nrdH"
                     /locus_tag="ACHURS_000203"
     CDS             2040..2285
                     /gene="nrdH"
                     /locus_tag="ACHURS_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFR1236039.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            2282..2692
                     /gene="nrdI"
                     /locus_tag="ACHURS_000204"
                     /pseudo
     CDS             2282..2692
                     /gene="nrdI"
                     /locus_tag="ACHURS_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
     gene            2665..4809
                     /gene="nrdE"
                     /locus_tag="ACHURS_000205"
     CDS             2665..4809
                     /gene="nrdE"
                     /locus_tag="ACHURS_000205"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR1236040.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEIL
                     QVNCASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            4819..5778
                     /gene="nrdF"
                     /locus_tag="ACHURS_000206"
     CDS             4819..5778
                     /gene="nrdF"
                     /locus_tag="ACHURS_000206"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR1236041.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            6134..7336
                     /gene="proV"
                     /locus_tag="ACHURS_000207"
     CDS             6134..7336
                     /gene="proV"
                     /locus_tag="ACHURS_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFR1236042.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAKLRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            7329..8393
                     /gene="proW"
                     /locus_tag="ACHURS_000208"
     CDS             7329..8393
                     /gene="proW"
                     /locus_tag="ACHURS_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFR1236043.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNSFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            8451..9443
                     /gene="proX"
                     /locus_tag="ACHURS_000209"
     CDS             8451..9443
                     /gene="proX"
                     /locus_tag="ACHURS_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFR1236044.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYCEGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            9734..10918
                     /locus_tag="ACHURS_000210"
     CDS             9734..10918
                     /locus_tag="ACHURS_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1236045.1"
                     /translation="MTKPNHELSPALIVLMSIATGQAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            11042..11779
                     /gene="ygaZ"
                     /locus_tag="ACHURS_000211"
     CDS             11042..11779
                     /gene="ygaZ"
                     /locus_tag="ACHURS_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFR1236046.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            11769..12104
                     /gene="ygaH"
                     /locus_tag="ACHURS_000212"
     CDS             11769..12104
                     /gene="ygaH"
                     /locus_tag="ACHURS_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFR1236047.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            12195..12725
                     /gene="emrR"
                     /locus_tag="ACHURS_000213"
     CDS             12195..12725
                     /gene="emrR"
                     /locus_tag="ACHURS_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFR1236048.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            12852..14024
                     /gene="emrA"
                     /locus_tag="ACHURS_000214"
     CDS             12852..14024
                     /gene="emrA"
                     /locus_tag="ACHURS_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFR1236049.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYCFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            14041..15579
                     /gene="emrB"
                     /locus_tag="ACHURS_000215"
     CDS             14041..15579
                     /gene="emrB"
                     /locus_tag="ACHURS_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFR1236050.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(15643..16158)
                     /gene="luxS"
                     /locus_tag="ACHURS_000216"
     CDS             complement(15643..16158)
                     /gene="luxS"
                     /locus_tag="ACHURS_000216"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFR1236051.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(16308..17864)
                     /gene="gshA"
                     /locus_tag="ACHURS_000217"
     CDS             complement(16308..17864)
                     /gene="gshA"
                     /locus_tag="ACHURS_000217"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFR1236052.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGDAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHT"
     gene            complement(17937..18365)
                     /locus_tag="ACHURS_000218"
     CDS             complement(17937..18365)
                     /locus_tag="ACHURS_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFR1236053.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKAIGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(18362..18928)
                     /gene="yqaB"
                     /locus_tag="ACHURS_000219"
     CDS             complement(18362..18928)
                     /gene="yqaB"
                     /locus_tag="ACHURS_000219"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFR1236054.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVDVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRHYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(19209..19285)
                     /locus_tag="ACHURS_000220"
     tRNA            complement(19209..19285)
                     /locus_tag="ACHURS_000220"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19249..19251),aa:Arg,seq:acg)
     gene            complement(19484..19560)
                     /locus_tag="ACHURS_000221"
     tRNA            complement(19484..19560)
                     /locus_tag="ACHURS_000221"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19524..19526),aa:Arg,seq:acg)
     gene            complement(19624..19700)
                     /locus_tag="ACHURS_000222"
     tRNA            complement(19624..19700)
                     /locus_tag="ACHURS_000222"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19664..19666),aa:Arg,seq:acg)
     gene            complement(19704..19796)
                     /locus_tag="ACHURS_000223"
     tRNA            complement(19704..19796)
                     /locus_tag="ACHURS_000223"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19760..19762),aa:Ser,seq:gct)
     gene            complement(20112..20297)
                     /gene="csrA"
                     /locus_tag="ACHURS_000224"
     CDS             complement(20112..20297)
                     /gene="csrA"
                     /locus_tag="ACHURS_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFR1236055.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(20532..23162)
                     /gene="alaS"
                     /locus_tag="ACHURS_000225"
     CDS             complement(20532..23162)
                     /gene="alaS"
                     /locus_tag="ACHURS_000225"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFR1236056.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(23290..23790)
                     /gene="recX"
                     /locus_tag="ACHURS_000226"
     CDS             complement(23290..23790)
                     /gene="recX"
                     /locus_tag="ACHURS_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFR1236057.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALVRDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(24018..25079)
                     /gene="recA"
                     /locus_tag="ACHURS_000227"
     CDS             complement(24018..25079)
                     /gene="recA"
                     /locus_tag="ACHURS_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFR1236058.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(25159..25656)
                     /gene="pncC"
                     /locus_tag="ACHURS_000228"
     CDS             complement(25159..25656)
                     /gene="pncC"
                     /locus_tag="ACHURS_000228"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFR1236059.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(25801..26886)
                     /gene="mltB"
                     /locus_tag="ACHURS_000229"
     CDS             complement(25801..26886)
                     /gene="mltB"
                     /locus_tag="ACHURS_000229"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFR1236060.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDRMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            27142..27705
                     /gene="srlA"
                     /locus_tag="ACHURS_000230"
                     /gene_synonym="gutA"
     CDS             27142..27705
                     /gene="srlA"
                     /locus_tag="ACHURS_000230"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFR1236061.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            27702..28661
                     /gene="srlE"
                     /locus_tag="ACHURS_000231"
                     /gene_synonym="gutE"
     CDS             27702..28661
                     /gene="srlE"
                     /locus_tag="ACHURS_000231"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFR1236062.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSSPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSHFLTGAPTVLIAWFVSGFIYQ"
     gene            28672..29043
                     /gene="srlB"
                     /locus_tag="ACHURS_000232"
                     /gene_synonym="gutB"
     CDS             28672..29043
                     /gene="srlB"
                     /locus_tag="ACHURS_000232"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFR1236063.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            29047..29815
                     /gene="srlD"
                     /locus_tag="ACHURS_000233"
                     /pseudo
     CDS             29047..29815
                     /gene="srlD"
                     /locus_tag="ACHURS_000233"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
     gene            29921..30280
                     /gene="gutM"
                     /locus_tag="ACHURS_000234"
     CDS             29921..30280
                     /gene="gutM"
                     /locus_tag="ACHURS_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFR1236064.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            30347..31120
                     /gene="srlR"
                     /locus_tag="ACHURS_000235"
                     /gene_synonym="gutR"
     CDS             30347..31120
                     /gene="srlR"
                     /locus_tag="ACHURS_000235"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFR1236065.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            31113..32078
                     /gene="gutQ"
                     /locus_tag="ACHURS_000236"
     CDS             31113..32078
                     /gene="gutQ"
                     /locus_tag="ACHURS_000236"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFR1236066.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTSAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(32075..33589)
                     /gene="norR"
                     /locus_tag="ACHURS_000237"
     CDS             complement(32075..33589)
                     /gene="norR"
                     /locus_tag="ACHURS_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFR1236067.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VMAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGMKD"
     gene            33776..35215
                     /gene="norV"
                     /locus_tag="ACHURS_000238"
     CDS             33776..35215
                     /gene="norV"
                     /locus_tag="ACHURS_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFR1236068.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTKSDLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            35212..36345
                     /gene="norW"
                     /locus_tag="ACHURS_000239"
     CDS             35212..36345
                     /gene="norW"
                     /locus_tag="ACHURS_000239"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFR1236069.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLHLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNDDIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(36473..38725)
                     /gene="hypF"
                     /locus_tag="ACHURS_000240"
     CDS             complement(36473..38725)
                     /gene="hypF"
                     /locus_tag="ACHURS_000240"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFR1236070.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLREDPETLLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTISQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLAAFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(38878..39405)
                     /gene="hydN"
                     /locus_tag="ACHURS_000241"
     CDS             complement(38878..39405)
                     /gene="hydN"
                     /locus_tag="ACHURS_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFR1236071.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(39553..40563)
                     /gene="ascG"
                     /locus_tag="ACHURS_000242"
     CDS             complement(39553..40563)
                     /gene="ascG"
                     /locus_tag="ACHURS_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFR1236072.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            40823..42280
                     /gene="ascF"
                     /locus_tag="ACHURS_000243"
     CDS             40823..42280
                     /gene="ascF"
                     /locus_tag="ACHURS_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFR1236073.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRVVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSVQPTVAKEVSLN"
     gene            42289..43713
                     /gene="ascB"
                     /locus_tag="ACHURS_000244"
     CDS             42289..43713
                     /gene="ascB"
                     /locus_tag="ACHURS_000244"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFR1236074.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGTYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(43827..44246)
                     /locus_tag="ACHURS_000245"
     CDS             complement(43827..44246)
                     /locus_tag="ACHURS_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1236075.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(44243..44554)
                     /locus_tag="ACHURS_000246"
     CDS             complement(44243..44554)
                     /locus_tag="ACHURS_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HFR1236076.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            complement(44716..45489)
                     /locus_tag="ACHURS_000247"
     CDS             complement(44716..45489)
                     /locus_tag="ACHURS_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1236077.1"
                     /translation="MNIRTGMCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLL
                     TMPYDAEQTWHFRKVGVAGVTLEKMPNDDSEWQLNGKDRAGKSWSVPVGMLANIAGKG
                     QLYRADLDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTG
                     VDDLLDLQGNGHTQLLDMQFDSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYT
                     PNRKLVLKPIAGRDPQTEDLAQTQRCLIKGDVLDGVNQN"
     gene            complement(45514..45984)
                     /gene="hycI"
                     /locus_tag="ACHURS_000248"
     CDS             complement(45514..45984)
                     /gene="hycI"
                     /locus_tag="ACHURS_000248"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFR1236078.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(45977..46387)
                     /gene="hycH"
                     /locus_tag="ACHURS_000249"
     CDS             complement(45977..46387)
                     /gene="hycH"
                     /locus_tag="ACHURS_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFR1236079.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(46384..47151)
                     /gene="hycG"
                     /locus_tag="ACHURS_000250"
     CDS             complement(46384..47151)
                     /gene="hycG"
                     /locus_tag="ACHURS_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFR1236080.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQQAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(47151..47693)
                     /gene="hycF"
                     /locus_tag="ACHURS_000251"
     CDS             complement(47151..47693)
                     /gene="hycF"
                     /locus_tag="ACHURS_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFR1236081.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(47703..49400)
                     /gene="hycE"
                     /locus_tag="ACHURS_000252"
     CDS             complement(47703..49400)
                     /gene="hycE"
                     /locus_tag="ACHURS_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFR1236082.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDELYPLRKDSMDYRQRPAPTTD
                     AETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEK
                     LAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVER
                     LHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIRRDLL
                     KDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEMARDFSNVGPMVRA
                     SGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLDNLPG
                     GPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPTLRYM
                     LRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNSPLK"
     gene            complement(49418..50341)
                     /gene="hycD"
                     /locus_tag="ACHURS_000253"
     CDS             complement(49418..50341)
                     /gene="hycD"
                     /locus_tag="ACHURS_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFR1236083.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIFKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWTGFGFAFLAFVSLLAA"
     gene            complement(50344..52170)
                     /gene="hycC"
                     /locus_tag="ACHURS_000254"
     CDS             complement(50344..52170)
                     /gene="hycC"
                     /locus_tag="ACHURS_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFR1236084.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLVIACWLLWQRYGTLDLCLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(52167..52778)
                     /gene="hycB"
                     /locus_tag="ACHURS_000255"
     CDS             complement(52167..52778)
                     /gene="hycB"
                     /locus_tag="ACHURS_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFR1236085.1"
                     /translation="MNRFVIVDSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRIDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(52903..53364)
                     /gene="hycA"
                     /locus_tag="ACHURS_000256"
     CDS             complement(52903..53364)
                     /gene="hycA"
                     /locus_tag="ACHURS_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFR1236086.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            53576..53926
                     /gene="hypA"
                     /locus_tag="ACHURS_000257"
     CDS             53576..53926
                     /gene="hypA"
                     /locus_tag="ACHURS_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFR1236087.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            53930..54802
                     /gene="hypB"
                     /locus_tag="ACHURS_000258"
     CDS             53930..54802
                     /gene="hypB"
                     /locus_tag="ACHURS_000258"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFR1236088.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            54793..55065
                     /gene="hypC"
                     /locus_tag="ACHURS_000259"
     CDS             54793..55065
                     /gene="hypC"
                     /locus_tag="ACHURS_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFR1236089.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            55065..56186
                     /gene="hypD"
                     /locus_tag="ACHURS_000260"
     CDS             55065..56186
                     /gene="hypD"
                     /locus_tag="ACHURS_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFR1236090.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQENEA"
     gene            56183..57193
                     /gene="hypE"
                     /locus_tag="ACHURS_000261"
     CDS             56183..57193
                     /gene="hypE"
                     /locus_tag="ACHURS_000261"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFR1236091.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEVALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            57267..59344
                     /gene="flhA"
                     /locus_tag="ACHURS_000262"
                     /pseudo
     CDS             57267..59344
                     /gene="flhA"
                     /locus_tag="ACHURS_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
     gene            complement(59381..59734)
                     /locus_tag="ACHURS_000263"
     CDS             complement(59381..59734)
                     /locus_tag="ACHURS_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFR1236092.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(59811..59945)
                     /locus_tag="ACHURS_000264"
     CDS             complement(59811..59945)
                     /locus_tag="ACHURS_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1236093.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            60021..62582
                     /gene="mutS"
                     /locus_tag="ACHURS_000265"
     CDS             60021..62582
                     /gene="mutS"
                     /locus_tag="ACHURS_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFR1236094.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            62688..63101
                     /locus_tag="ACHURS_000266"
                     /pseudo
     CDS             62688..63101
                     /locus_tag="ACHURS_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417214.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
BASE COUNT        14964 a        17013 c        16277 g        14847 t
ORIGIN      
        1 gcgagacgac gctgcgacgt tctgttcgcc atgatgcgcg acgggacttt ttataccccg
       61 caggggtcat aacatgcttg acaacttaat aggggcaccc ccctcactgg tgctttctgc
      121 tgttaatgta cattttctgg catcaaaaat gctgtaaatg acacggtcac gacatacacc
      181 gaattctcta cgcttatagc gttacctcac ccccaaactt tcattagtga aattaaaggc
      241 aaggtcatat ctaattgatt taattgaaaa taaatcatat cggcatgtaa aaatccacaa
      301 tgtacaaaaa acgagccgtt acggaatatt ttatctacaa aaactgacta aataaaaatt
      361 tttcactaat tgaatagtca tagccagcga tatacgctat gcgaaaatgc agatggcaat
      421 gagatccact gctttcatct ccattaacat cccattacgc ttttattaag gagcattagc
      481 atgttctcac cgcagtcacg cttgcgtcat gcagttgcag atacgttcgc gatggttgtt
      541 tactgttctg tcgtgaacat gtgtattgaa gttttcctct ccggaatgag cttcgaacag
      601 tctttttatt ccagattggt agcgattccg gtgaacatct taattgcatg gccatacggt
      661 atgtaccgcg atctatttat gcgcgcggca cgcaaagtta gcccgtcggg ctggataaaa
      721 aatctggctg atatcctggc ttatgtgacg ttccagtcac cggtgtatgt ggcgatcttg
      781 ttagtggtgg gcgcagactg gcatcagatt atggcggcgg tcagttcaaa catcgttgtt
      841 tcgatgttga tgggggcggt ttatggctac ttcctcgatt attgccgccg actgtttaaa
      901 gtcagccatt accagcaggt aaaagcctga ctcttctttt cgcgactggc atcgccagtc
      961 gcgtctttaa ctggcttcgc caaataaacc attcaaataa cgttcaagcg caacacgcga
     1021 actgaagcca tgcgggatac cgtaatgctc gtgtcgctcg cctgctaaat agagcggaaa
     1081 ttgttgataa tgatcgcaag ttttaatatc ggaagcgggt tcagccagtg tcgaactacg
     1141 ttcttttaat gttggatgaa ttaccagtgc ggtacgtccc attcgcgctt cgcgattaac
     1201 atagacataa ttttccccac ggcggtaacc ataagctttc tgggttacca catcgacagt
     1261 aaaaccgact ttttcaagta cgcgcgccac ctcgtctggt cgtaaataca tatttgatcc
     1321 tcgttattat caaccgcggg cctaccttac ctgattgcgc attcacaacg ctttcagaaa
     1381 agtccataaa gccgcgatga acctcagtta agaaaattat ggtctacact gaaaattaca
     1441 tcgaattcta atgggggatc atatgtttaa ccgaccgaac cgcaacgacg ttgatgatgg
     1501 cgtgcaggat atccagaatg atgtcaatca attagctgac agcctcgaat ctgtattgaa
     1561 gtcctggggc agcgacgcca aaggggaagc tgaagccgca cgcagtaaag ctcaggcatt
     1621 gctgaaagaa accagggcgc gaatgcatgg tcgtactcgc gtccagcaag ccgcacgcga
     1681 tgccgttggc tgcgcggatt cttttgttcg tgaaagaccc tggtgtagcg tgggtacagc
     1741 agctgcggta ggtattttta tcggcgcact gttaagcatg cgcaaatcgt agtgcaaaaa
     1801 tgataataaa tacgcgtctt taaccccgaa gcctgtcttc ggggtttctt tttgcctggt
     1861 gaatcacaaa aatccccctc ccccgtcacg ctcatatcta gggtaatttc gatcactatt
     1921 tgctatatat tgtgtggttg aatctttttt caactacatc tagtatctct gtatcaacag
     1981 agagacaacc cgacgcgtat catcgcgccg tatcttcatt ttaaacggaa atacgaatca
     2041 tgcgcataac tatttacact cgtaacgatt gcgttcagtg ccacgccacc aaacgggcga
     2101 tggaaaaccg gggctttgat tttgaaatga ttaatgtcga tcgcgttcct gaagcagcgg
     2161 aagcgttgcg tgctcagggc tttcgtcagt tgccggtagt gattgctggc gatcttagct
     2221 ggtctggttt ccgtccggac atgattaacc gtctgcatcc agcgccacac gcggccagtg
     2281 catgagccag ctcgtctact tctccagcag ctccgaaaac acgcagcgtt ttatcgaacg
     2341 tttaggtctg cccgcggtgc gcatcccgct caatgagcgg gaacggattc aggtagacga
     2401 gccttacatc ctgatcgtgc cctcttacgg cggcggcggt acggctggcg cggtgccacg
     2461 ataggtaatt cgctttttaa acgacgagca caaccgggcg ttgcttcgcg gcgttattgc
     2521 ttctggtaat cgcaactttg gtgaggcgta tggccgcgcc ggagatgtga ttgcccggaa
     2581 atgcggcgtg ccgtggctgt accgttttga actcatgggt acgcaaagcg atatcgaaaa
     2641 cgttcgtaaa ggagtaaccg aattttggca acgacaaccg cagaatgcct gacgcaggaa
     2701 acgatggatt accacgcgct gaatgcgatg cttaacctct acgatagcgc aggtcgcatt
     2761 cagttcgata aagaccgcca ggccgttgac gcctttatgg cgacgcatgt gcgtccgaac
     2821 agtgtgacct tcagtagcca gcagcagcgc ctgaactggc tggtcaacga aggttactat
     2881 gatgaaagcg ttcttaatcg ctactctcgc gactttgtca ttacgctgtt tgcccacgca
     2941 cacaccagcg gttttcgttt ccagacattc ctcggggcat ggaagtttta caccagctat
     3001 acgttgaaga cattcgacgg taaacgttat ctggaagatt ttgccgatcg agtcacgatg
     3061 gtggcgctga cgctggcaca aggcgatgcg acgctggcgt tgcaactgac cgatgaaatg
     3121 ctgtcaggac gctttcagcc agccacgccg acattcctca actgcggtaa gcagcagcgc
     3181 ggcgaactgg tttcctgttt tctgctgcgt attgaagaca atatggagtc gattggccgg
     3241 gcggtaaatt ccgcactgca actgtcgaaa cgcggcggcg gcgtggcatt tttgctgtcg
     3301 aatctgcgag aagcgggcgc gccaattaaa cgtattgaaa atcaatcttc tggcgtaatt
     3361 ccggtgatga aaatgctgga agacgcattt tcctatgcca accaactcgg cgcgcgtcag
     3421 ggggcaggtg cagtctattt acatgctcat catcccgata ttctgcgttt tctcgataca
     3481 aaacgggaaa atgccgacga aaaaatccgc attaaaacac tgtcgctggg cgtggtgatc
     3541 ccggatatca ctttccatct ggcaaaagag aatgcgcaga tggcgctgtt ttcgccctat
     3601 gacgttgagc gagtttatgg caagccgttt gccgatatcg ccatcagcga acactatgac
     3661 gaactggttg ccgatgaacg catccgcaaa aaatacctca acgcccgtga tttcttccag
     3721 cggctggcag aaatccagtt tgagtccggc tatccctaca tcatgtatga agacacggta
     3781 aaccgtgcta accctgtcgc cgggcgcata aatatgagta atctctgctc agagattttg
     3841 caggtcaact gtgcctcaga gtatgacgag aatctcgact atgcccgcac aggccatgat
     3901 atctcctgca atttaggttc gttgaatatt gcgcacacca tggattcccc cgattttgcc
     3961 cgcacggtag agactgccgt gcgcggttta acggcggtgt cagatatgag tcatatccgc
     4021 agcgtgccgt ccatcgaagc cggaaatgcc gcctcacacg ccatcggact ggggcagatg
     4081 aatttacacg gctatctggc gcgagaaggc atcgcttatg gttcgccgga agcactggat
     4141 ttcaccaatc tctatttcta tgccatcacc tggcacgcac tgcgtacctc gatgttgctg
     4201 gcgcgcgaac gcggtgaaac cttcgccggg ttcaaacagt cacgttatgc cagtggtgaa
     4261 tattttagcc aatatctgca aggtaactgg cagccgaaaa cggcgaaagt tggcgaactg
     4321 tttgcccgca gcggtattac gttacctacc cgtgagatgt gggcgcaact gcgcgacgac
     4381 gtgatgcgct acggcattta caaccagaat cttcaggcgg tgccgccaac cggttctatc
     4441 tcttatatca accatgctac gtcgagtatt catccgattg tggcgaaagt agagatacgc
     4501 aaagagggca aaacaggacg cgtttactac cctgccccgt ttatgactaa cgaaaatctg
     4561 gcgctgtatc aggatgctta cgaaatcggc gcagaaaaga tcatcgacac ctacgcggaa
     4621 gcgactcgcc atgtcgatca gggtttgtcg ctgacgctgt ttttccccga taccgccacc
     4681 actcgcgata tcaacaaagc gcagatttac gcctggcgca aaggtatcaa aacgctctat
     4741 tacattcgcc tgcgtcagat ggcgctggaa ggcactgaaa ttgaaggctg cgtctcctgt
     4801 gcactttaag gaatatctat gaaactctca cgtatcagcg ccatcaactg gaacaagata
     4861 tctgacgata aagatctgga ggtgtggaat cgcctgacca gcaatttctg gctaccagaa
     4921 aaggtgccgc tgtcgaacga tattcctgcc tggcagacat taactgtcgt agaacaacaa
     4981 ctgacgatgc gcgtttttac tggcctgacg ctgctcgaca cgctgcaaaa tgttatcggc
     5041 gcgccttcgc tgatgcccga tgcactcacg cctcatgaag aagcggtatt atcgaatatc
     5101 agctttatgg aagcggttca tgcccgctct tacagttcga ttttctcgac gctgtgccag
     5161 accaaagatg tcgatgccgc ctacgcctgg agtgaagaaa acgcaccgtt gcagcgaaaa
     5221 gctcagatta ttcagcaaca ttatcgcggt gatgatccgc tgaaaaagaa aatcgccagt
     5281 gtgtttcttg aatctttttt gttctattcc ggtttctggc tgccgatgta tttttccagc
     5341 cgcggaaagc tgaccaatac cgcagacctg atccgtctga ttatccgtga tgaagcagtc
     5401 cacggttact acataggcta taaatatcag aaaaacatgg aaaagatatc tctgggacaa
     5461 cgtgaagagt tgaagagttt cgccttcgat ttgttgctgg aactctacga caacgagctg
     5521 caatacaccg atgagctgta cgccgaaacc ccgtgggctg acgatgtgaa agcgtttctc
     5581 tgttacaacg ccaataaggc tttgatgaat ctgggctacg aacctttatt tcccgcagaa
     5641 atggcggaag tgaatccggc aatcctcgcc gcgctttcgc cgaatgccga tgaaaatcac
     5701 gatttctttt ccggttcagg ctcctcttat gtgatgggga aagcggttga aacagaagat
     5761 gaagactgga atttctgagg gtgttatttt caaaaatatc actacccgcg acagggaaat
     5821 aattcccgcc aaatagcttt ttatcacgca aatattttgt ggtgatctac actgatactc
     5881 tgttgcatta ttcgcctgaa accacaatat tcaggcgttt tttcgctatc tttgacaaaa
     5941 aatatcaact tttctcgatt tgctctcagc ccttatatca cgggaaattc cggcgatttg
     6001 ctcgcatcaa tattcatgcc acatttgcca tcaggggttg cctcagattc tcagtatgtt
     6061 agggtagaaa aaagtaacta tttccattgg gtaatatatc gacataaaca aataacagga
     6121 atctttctat tgcatggcaa ttaaattaga aattaaaaac ctttataaaa tatttggtga
     6181 gcatccacag cgagcattca aatatatcga acaaggactt tcaaaagaac aaattctgga
     6241 aaaaactggg ctatcgcttg gcgtaaaaga cgccagtctg gccattgaag aaggcgagat
     6301 atttgtcatc atgggattat ccggctcggg taaatccaca atggtacgcc ttctcaatcg
     6361 cctgattgaa cccacccgcg ggcaagtgct gattgatggt gtggatattg ccaaaatatc
     6421 cgatgccaaa ctccgtgagg tgcgcagaaa aaagattgcg atggtcttcc agtcctttgc
     6481 cttaatgccg catatgaccg tgctggacaa tactgcgttc ggtatggaat tggccggaat
     6541 taatgccgaa gaacgccggg aaaaagccct tgatgcactg cgtcaggtcg ggctggaaaa
     6601 ttatgcccac agctacccgg atgaactctc tggcgggatg cgtcaacgtg tgggattagc
     6661 ccgcgcgtta gcgattaatc cggatatttt attaatggac gaagccttct cggcgctcga
     6721 tccattaatt cgcaccgaga tgcaggatga gctggtaaaa ttacaggcga aacatcagcg
     6781 caccattgtc tttatttccc acgatctcga tgaagccatg cgtattggcg accgaattgc
     6841 cattatgcaa aatggtgaag tggtacaggt cggcacaccg gatgaaattc tcaataatcc
     6901 ggcgaatgat tatgtccgta ccttcttccg tggcgttgat attagtcagg tattcagtgc
     6961 gaaagatatt gcccgccgga caccgaatgg cttaattcgt aaaacccctg gcttcggccc
     7021 acgttcggca ctgaaattat tgcaggatga agatcgcgaa tatggctacg ttatcgaacg
     7081 cggtaataag tttgtcggcg cagtctccat cgattcgctt aaaaccgcgt taacgcagca
     7141 gcaaggtctt gatgcggcgc tgattgatgc gccgttagca gtcgatgcac aaacgcctct
     7201 tagcgagttg ctctctcatg tcggacaggc accctgtgcg gtgcccgtgg tcgacgagga
     7261 ccaacagtat gtcggcatca tttcgaaagg aatgctgctg cgcgctttag atcgtgaggg
     7321 ggtaaataat ggctgatcaa aataatccgt gggataccac gccagcggcg gacagtgccg
     7381 cgcaatccgc agacgcctgg ggtacaccga cgactgcacc gactgacggc ggtggtgctg
     7441 actggctgac cagtacgcct gcgccaaacg tcgagcattt taatattctc gatccgttcc
     7501 ataaaacgct gatcccgctc gacagttggg tcactgaagg gatcgactgg gttgttaccc
     7561 atttccgtcc cgtcttccag ggcgtgcgcg ttccggttga ttatatcctc aacagtttcc
     7621 agcaattgct gctgggtatg ccagcgccag tggcgattat cgttttcgct ctcatcgcct
     7681 ggcagatttc cggggtcgga atgggcgtgg cgacgctggt ttcgctgatt gccatcggcg
     7741 caatcggtgc ctggtcgcag gcaatggtga ctctggcgct ggtgttaacc gccctgctgt
     7801 tctgtatcgt catcggtttg ccgttgggga tatggctggc gagaagtccg cgagcggcga
     7861 aaattattcg tccactgctt gatgccatgc agaccacgcc agcgtttgtt tatctggtac
     7921 caatcgtcat gctgtttggt atcggtaacg tgccgggcgt agtggtgacg atcatctttg
     7981 ctctgccgcc gattatccgt ctgacgattc tggggattaa ccaggttccg gcggatctga
     8041 ttgaagcctc gcgctcattc ggtgccagcc cgcgccagat gctgttcaaa gttcagttac
     8101 cgctggcgat gccgaccatt atggcgggcg ttaaccagac gctgatgctg gctctttcta
     8161 tggtggtcat cgcctcgatg attgccgtcg gcgggctggg tcagatggta cttcgcggta
     8221 tcggtcgtct ggatatgggc cttgccaccg ttggcggcgt cgggattgtg atcctcgcca
     8281 ttatcctcga ccgcctgacg caggccgttg ggcgcgactc acgcagtcgc ggcaaccgtc
     8341 gctggtacac cactggccct gtcggtctgc tgacccgccc attcattaag taactctgca
     8401 cttgcccggt aacgccgggc attatcaccc tgccaaaaaa aggaataaca atgcgacata
     8461 gcgtactttt tgcgacagcg tttgccacgc ttatctctac acaaactttt gcagccgatc
     8521 tgccgggcaa aggcattact gttaatccag ttcagagcac catcactgaa gaaaccttcc
     8581 agacgctgct ggtcagtcgt gcactggaga aattaggtta taccgtcaat aaacccagcg
     8641 aagtggatta caacgttggc tacacctcac ttgcttccgg cgatgcaacc ttcaccgccg
     8701 tgaactggac gccgctgcat gacaacatgt acgaagctgc cggtggcgat aagaaatttt
     8761 attgtgaagg ggtattcgtt aacggcgcgg cacagggtta cctgatcgat aagaaaaccg
     8821 ccgaccagta caaaatcacc aacatcgcac aactgaaaga tccgaagatc gccaaactgt
     8881 tcgataccaa cggcgacggt aaagcggatt taaccggctg taacccaggc tggggctgcg
     8941 aaggtgcgat caaccaccag cttgccgcgt atgaactgac caacaccgtg acgcataatc
     9001 aggggaacta cgcagcgatg atggccgaca ccatcagtcg ctacaaagag ggcaaaccgg
     9061 tgttttatta cacctggacg ccgtactggg tgagtaatga gctgaagcca gggaaagatg
     9121 tggtctggtt gcaggtgccg ttctccgcac tgccgggcga taaaaacgcc gataccaaac
     9181 tgccgaatgg tgcgaattat ggcttcccgg tcagcaccat gcatatcgtt gccaacaaag
     9241 cctgggccga gaaaaacccg gcagcagcga aactttttgc cattatgcag ttgccagtgg
     9301 cagatattaa cgcccagaac gccattatgc atgacggcaa agcctcagaa ggcgatattc
     9361 agggccatgt tgatggttgg atcaaagccc accagcagca gttcgatggc tgggtgaatg
     9421 aggcgctggc agcgcagaag taatttttat tcgggcggat aagtcgtttt tgcctcatcc
     9481 gccattctgt gcacaatgcc tgatgcgacg ctggcgcgtc ttatcatgcc tacaaaatac
     9541 actcattccg tatggcggat aaggcgtttt cgccgcatcc gccgttctgt gcacaatgcc
     9601 tgatgcgacg ctggcgcgtc ttatcatgcc tacaaaccac atcgcacatt tcaacaagct
     9661 atctttcgcc cccattcatc aacatccgct attattgatt tccagcttaa tcatcacctg
     9721 atgaacaaaa ataatgacta aacctaatca tgagcttagc ccggcgctga tcgtgctgat
     9781 gtctatcgcc accggtcagg cggtagccag taactattac gcccagccat tgctcgacac
     9841 catcgcgcgt aacttttccc tttccgccag ttcggcaggc tttattgtta ccgccgcgca
     9901 gttgggctat gcagcaggtc tgctgtttct tgttcccctc ggtgatatgt ttgaacgccg
     9961 ccgcctgatt gtctcgatga ccttactggc ggcaggcggc atgttaatca ctgccagcag
    10021 tcagtcgctg gcgatgatga tcctcggtac ggcattaacc ggtttattct cagtcgtggc
    10081 acaaattctg gttccgctgg cagcgacgct ggcttcaccg gataaacgcg gcaaagtggt
    10141 tggcaccatt atgagcggtc tgctgttggg gatcttgctg gcacgaaccg ttgccgggtt
    10201 actggcaaac ctcggcggct ggcgcaccgt cttttgggtt gcctcggtgt taatggcact
    10261 gatggcgcta gcattatggc gtggtctgcc acaaatgaaa tcagaaaccc acctcaacta
    10321 cccacagttg ttaggttccg ttttcagcat gtttatcagc gataagatcc tccgcacccg
    10381 cgcattgctg ggctgcctga cctttgccaa cttcagcatt ctctggacct caatggcctt
    10441 tttgcttgcc gctccacctt ttaactacag cgatggtgtc attggtctgt ttggacttgc
    10501 gggagctgcc ggagcgttgg gcgctcgtcc ggcgggcggt tttgccgata agggtaaatc
    10561 gcaccacacc acaactttcg gtctgctgct gctattactt tcatggctgg cgatctggtt
    10621 tggtcacact tccgtactgg cgctgattat cggcatcctg gtgctggatc tcaccgtaca
    10681 gggcgtgcat atcactaacc agacggtaat ttatcgaata caccctgatg cgcgtaatcg
    10741 cctgaccgca ggttacatga ccagctactt tattggcggt gccgccggtt cgctaatttc
    10801 agcctcagcc tggcaacatg gcggttgggc tggcgtttgt ctggctggcg cgacgattgc
    10861 cctagttaac ttactggtct ggtggcgagg ttttcatcgt caggaagccg caaattaagc
    10921 aatcagcaac cgttttcgtg gctttacact tataagggtg ttaagaagcc catcagtctg
    10981 ataaggttaa gatattcatt cagtctattt ataatattaa caatcgttaa gcgtacactc
    11041 tatggaaagc cctactccac agcctgctcc tggttcggcg accttcatgg aaggatgcaa
    11101 agacagttta ccgattgtta ttagttatat tccggtggcc tttgcgttcg gtctgaatgc
    11161 gacccgtctg ggattctctc ctctcgaaag cgtttttttc tcctgcatca tttatgcagg
    11221 cgcgagccag ttcgtcatta ccgcgatgct ggcagccggg agtagtttgt gggttgctgc
    11281 actgaccgtc atggcaatgg atgttcgcca tgtgttgtat ggcccgtcac tgcgtagccg
    11341 tattattcag cgtctgcaaa aatcgaaaac cgccctgtgg gcgtttggcc tgacggatga
    11401 ggtttttgcc gccgcaacag caaaactggt acgcaataat cgccgctgga gcgagaactg
    11461 gatgatcggc attgccttca gttcatggtc atcgtgggta tttggtacgg taataggggc
    11521 attctccggc agcggcttgc tgcaaggtta tcccgccgtt gaagcagcat taggttttat
    11581 gcttccggca ctctttatga gtttcctgct cgcctctttc cagcgcaaac aatctctttg
    11641 cgttaccgca gcgttagttg gtgcccttgc aggcgtaacg ctattttcta ttcccgtcgc
    11701 cattctggca ggcattgtct gtggctgcct cactgcgtta atccaggcat tctggcaagg
    11761 agcgcccgat gagctatgag gttctgctgc ttgggttact ggttggcgcg gcgaattatt
    11821 gcttccgcta tttgccgctg cgcctgcgtg tgggtaatgc ccgcccaacc aaacgtggcg
    11881 cggtaggtat tttgctcgac accattggca tcgcctcgat atgcgctctg ctggttgtct
    11941 ctaccgcacc agaagtgatg cacgatacac gccgtttcgt gcccacgctg gtcggcttcg
    12001 cggtactggg tgccagtttc tataaaacac gcagcattat catcccaaca ctgcttagtg
    12061 cgctggccta tgggctcgcc tggaaagtga tggcgattat ataacccaca agaatcattt
    12121 ttctaaaaca atacatttac tttatttgtc actgtcgtta ctatatcggc tgaaattaat
    12181 gaggtcatac ccaaatggat agttcgttta cgcccattga acaaatgcta aaatttcgcg
    12241 ccagccgcca cgaagatttt ccttatcagg agatccttct gactcgtctt tgcatgcaca
    12301 tgcaaagcaa gctgctggag aaccgcaata aaatgctgaa ggctcagggg attaacgaga
    12361 cgttgtttat ggcgttgatt acgctggagt ctcaggaaaa ccacagtatt cagccttctg
    12421 aattaagttg tgctcttgga tcatcccgta ccaacgcgac gcgtattgcc gatgaactgg
    12481 aaaaacgcgg ttggatcgaa cgtcgtgaaa gcgataacga tcgccgctgc ctgcatctgc
    12541 aattaacgga aaaaggtcac gagtttttgc gcgaggtttt accaccgcag cataactgcc
    12601 tgcatcaact ctggtccgcg ctcagcacaa cagaaaaaga tcagctcgag caaatcaccc
    12661 gcaaattgct ctcccgtctc gaccagatgg aacaagacgg tgtggttctc gaagcgatga
    12721 gctaacgcgt catctcgctc aaaaatccag atttataaaa gaaaaaatga ccggccagca
    12781 tcgcaacatg ctggcctttt tggcaagcag gtcggctcag ccgatgagtt aagaagatcg
    12841 tggagaacaa tatgagcgca aatgcggaga ctcaaacccc gcagcaaccg gtaaagaaga
    12901 gcggcaaacg taagcgtctg ctcctccttc tcaccttgct ctttataatt attgccgtag
    12961 cgatagggat ttattgtttt ttggtactgc gtcatttcga agaaaccgat gacgcatacg
    13021 tggcagggaa tcagattcaa attatgtctc aggtgtctgg cagcgtgacg aaagtctggg
    13081 ccgataacac cgattttgta aaagaaggcg acgtgctggt cactctcgac ccgacagatg
    13141 ctcgccaggc gtttgaaaaa gccaaaactg cactggcttc cagcgttcgc caaacccacc
    13201 agctgatgat taacagcaaa cagttgcagg cgaatattga ggtgcagaaa atcgccctcg
    13261 caaaagcaca aagcgactac aaccgccgtg tgccgctggg caatgccaac ctgattggtc
    13321 gcgaagagct gcaacacgcc cgcgacgccg tcaccagtgc ccaggcgcaa ctggacgtcg
    13381 ctattcaaca atacaatgcc aatcaggcga tgattctggg gactaaactg gaagatcagc
    13441 ctgccgtgca acaggctgcc accgaagtac gtaacgcctg gctggcgctg gagcgtaccc
    13501 gtattgtcag tccgatgacc ggttatgtct cccgccgcgc ggtacagcct ggggcgcaaa
    13561 ttagcccaac gacgccgctg atggcggtcg ttccagccac caatatgtgg gtggatgcca
    13621 actttaaaga gacgcagatt gccaatatgc gtatcggtca gccggtcact atcaccacgg
    13681 atatttacgg cgatgatgtg aaatacaccg gtaaagtggt tggtctggat atgggcacag
    13741 gtagcgcatt ctcactgctt ccggcgcaaa atgcgaccgg taactggatc aaagtcgttc
    13801 agcgtctacc tgtgcgtatc gaactggacc agaaacagct ggagcaatac ccgctgcgca
    13861 ttggtttgtc cacactggtg agcgtcaata ccactaaccg tgacggtcag gtactggcaa
    13921 ataaagtacg ttcaacaccg gtcgcggtaa gtaccgcgcg tgaaatcagc ctggcacctg
    13981 tcaataaact gatcgacgat atcgtaaaag ctaacgctgg ctaatccaga ggtgcgtgtg
    14041 atgcaacagc aaaaaccgct ggaaggcgcg caactggtca ttatgacgat tgcgctgtca
    14101 ctggcgacat tcatgcaggt gctggactcc accattgcta acgtggcgat ccccactatc
    14161 gccgggaatc tgggctcatc gctcagccag ggaacgtggg taatcacttc tttcggggtg
    14221 gcgaatgcca tctcgatccc gcttaccggc tggctggcaa agcgcgtcgg ggaagtgaaa
    14281 ctgttccttt ggtccaccat cgcctttgct attgcgtcgt gggcgtgtgg tgtctccagc
    14341 agcctgaata tgctgatctt cttccgcgtg attcagggga ttgtcgccgg gccgttgatc
    14401 ccgctttcgc aaagtctatt gctgaataac tacccgccag ccaaacgctc gatcgcgctg
    14461 gcgttgtggt cgatgacggt gattgtcgcg ccaatttgcg gcccgatcct cggcggttat
    14521 atcagcgata attaccactg gggctggata ttcttcatca acgtgccgat tggcgtggcg
    14581 gtggtgttga tgacactgca aactctgcgc ggacgtgaaa cccgcaccga acggcggcgg
    14641 attgatgccg tggggctggc actgctggtt attggtatcg gcagcctgca gattatgctc
    14701 gaccgcggta aagagctgga ctggttttca tcacaggaaa ttatcatcct taccgtggtg
    14761 gcggtggtgg ctatctgctt cctgattgtc tgggagctga ccgacgataa cccgatagtc
    14821 gatctgtcgt tgtttaagtc gcgcaacttc accatcggct gcttgtgtat cagcctcgcg
    14881 tatatgctct acttcggcgc tattgttctg ctgccgcagt tgttgcagga ggtctacggt
    14941 tacacggcga cctgggcagg tttggcctct gcgccggtag ggattattcc ggtgatcctg
    15001 tcgccgatta tcggccgctt cgcgcataaa ctggatatgc ggcggctggt aaccttcagc
    15061 tttattatgt atgccgtctg cttctactgg cgtgcctata cctttgaacc aggtatggat
    15121 tttggcgcgt cggcctggcc gcagtttatc caggggtttg cggtggcctg cttctttatg
    15181 ccgctgacca ccattacgct gtctggtttg ccaccggaac gactggcggc ggcatcgagc
    15241 ctctctaact ttacgcgaac gctggcgggg tctatcggca cgtcgataac cacgaccatg
    15301 tggaccaacc gcgagtcgat gcaccatgcg cagttgactg agtcggtaaa cccgttcaac
    15361 ccgaatgccc aggcgatgta cagtcaactg gaagggcttg ggatgacgca acagcaggca
    15421 tcaggctgga ttgcccagca gatcaccaat caggggctga ttatttccgc caatgagatc
    15481 ttctggatgt cagccgggat attcctcgta ctgctggggc tggtgtggtt tgctaaaccg
    15541 ccatttggcg caggtggcgg tggcggcggt gcgcactaag tacaactaag ccagttcatt
    15601 tgaactggct tttttcaatt aattgtgaag atagtttact gactagatgt gcagttcctg
    15661 caacttctct ttcggcagtg ccagttcttc gttgctgttg atgcgtacgt cacgttccag
    15721 aatgctacgc gcaatatcct gcgcttcctg caacgagtgc atctggtaag tgccacactg
    15781 gtagacgttc agttccggga tctgattctg atcctgcact ttcagcacgt cttccattgc
    15841 cgctttccag gcatcagcaa cacgctgctc atctggcgta ccaatcagac tcatataaaa
    15901 accggtgcgg cagcccattg gcgagatatc gataatctct acaccattac cgttaagatg
    15961 gttacgcata aaaccagcaa acaggtgctc cagggtatgg atccctcttt ctggcatcac
    16021 ttctttgttc ggcacgcaga agcgcagatc gaacacggtg attgcgtcgc catgcggggt
    16081 gttcattgtt ttcgccaccc gaactgcagg cgcttccatc cgggtatgat cgactgtgaa
    16141 gctatctaac aacggcattt agccacctcc ggtaattttt ttaaaaattt tctgaactct
    16201 ttcttcccag gcgagtctga gtatatgaaa gacgcgcatt tgttatcatc atccctgaat
    16261 tcagagatga aattttggcc actcacgagt ggcctttttc ttttctgtca ggtgtgtttt
    16321 tccagccaca ccgcaaacgg ttcggtatca gcggtttcca tttcctgctg acggcgttca
    16381 gatgcctcgc gctcggctac aaaatcctct tcgcgcagaa tttccagcgg ctcttcacgc
    16441 agcagattac ggtaggcttc tgcaaacgct ttgcctgttc cgccaatacc agtatcaatc
    16501 atagacctta agatacgggc agagaaagtc agatcgggat tatcgaagca ggcaaccagt
    16561 tcatcacaca ctttctgata cgcgtcgccg ccgttaatgc tatccagcgt ttgcgcgacg
    16621 cgtttcagat cgcggaacag atctttaccc acctgcagta acgggaactg cgcggtttcg
    16681 cagccgatac ccagcgtcag gcccggtttg cgaccttcaa ggatcacccg gttccagtta
    16741 acgcgcgtac aggcaagttc gctactgctc atttccggtg catcagccag cgcacaccag
    16801 accataaaca ggtcgaggaa tcgcacctgc tgttcatcta caccaatcgg cgagaacggg
    16861 ttgatgtcca gcgaacgcac ttcaatatat tcaatgccgc cacgtaacag cgcatcagaa
    16921 ggtgactcgc cgctgcgggt aacgcgtttt ggacgaatcg gcgcatacag ttcgttttca
    16981 atctgcagca cgttgctatt gatttgcagc ctcttaccgt ctttctcaat accaatcttc
    17041 gcgtactctt ccgatggcgt tttgattgcc tgtttaaggc ccgctacgta ctcgtaaaga
    17101 tcgttgaagg taataccaag attgctttgc gatttattgg tatagccgag atcgctcaaa
    17161 cgaagagagg tcgcatacgg caggtaatac ataccgcact cggttttctc aaacggcagc
    17221 gacgttggtt ttccttgcag gaaagaagaa caaatcgccg gagatgcacc aaacagataa
    17281 ggaatgaccc aaccgaaacg atagtaattg cggataacgc ggaaatagcc cgcagaaatt
    17341 ttctctttgg catcagcgcc cgagatatca ccgcacttcg cttgccagaa tgccattggc
    17401 aaagagaaat tgtagtgcac gccggaaatg gtttgcatca gcgcgccgta gcgatttttc
    17461 agcccttcac gatacagcgt tttaaagcgt ccggtgttag aagtgccgta ctgtgccagt
    17521 tcgatgtcct gaccttctgc gatgtagcat ggcatactta acggccacat ccgctcatcg
    17581 cccatattgc gcgccgtata acgatgcaga tcgcgcataa aggtcagcat atgttcaata
    17641 tcaccatcca ctggtgtaat gaattccagc aatgcttccg caaaatcggt agtaatccat
    17701 ttgtgcgtca gtgcggaacc taatgcttca ggatgacctg ttgttgccag tgtgccatca
    17761 gcattaacac gcaaagtttc gcgctccagc ccacgctgta tcccctttaa cgcctgagga
    17821 tgtttttcca gccaggccag cgcctgtgat acgtccggga tcaaattgac ctcccgcctg
    17881 tcaaaatcgt tttaattagc ataactgtaa tggtgaccat atgtgcaggc ctacaattag
    17941 tgccaccaca tcatgccctg aacggtcgct gctgcaactg caacatagcg taacgcttta
    18001 ccaaggcaca aaaaaaagat taccggtccc cacgagatgc gcatccatcc cgctaacaga
    18061 cacagtaaat cgccaaccac gggcatccag cttaataata gcgtgactgc accatagcgt
    18121 ttcagccagc cgatagcttt ctcttgccag cgcgatgttt tacgcaatgg aaagaaacgc
    18181 ccaaggataa cgttagttaa ccctccaagg ctattaccca ttgttgctgt taaaactaaa
    18241 acccagggat gactgatccc ggaaagcaac attgccacca gcacgacttc ggagttgccg
    18301 ggtaatagtg tagcgctgag aaaactactg gcaaacaacg agaaaagcga taacgcttca
    18361 ctcacagcaa gcgaacgtcc acagcgtcca tacctgcggc acgggccgcc tgaataccga
    18421 aatcggcatc ttcaaagacc acacactgcg tcggttgcac gcccatacgc tgcgcgcaca
    18481 acaaaaatgt gtctggcgcg ggtttatggt gtttgacgtg atcggcagcg acgacggcgt
    18541 caaaataatg gcgtaatccc aggtgcgcca gcaatgcctc agcgatggcg ctttcactcc
    18601 ccgttcctac agccattggg cgacgaccat gccaactttt caccacatca acaagaggaa
    18661 gcggttcgac gctatccagc agcatacttc ttactgcttc tgttttttca cgcgctaacg
    18721 catgcgggtc gagatcggcc tgattcagct caataattgc ctgagcaata cgccaggtgg
    18781 gcgatccatt aagcgcaatc atcgcctgaa tatcgtactg aagaccgtag tgccctaata
    18841 cttcgcgcca cgctttacgg tgcgtaggct ccgtatccag gattgtgcca tccatatcaa
    18901 aaattaaacc tgcataacgc tcgtacatgg tcttctcgca agtcgaaaaa tatgacgcta
    18961 ctttagcgta attgcttgat tttgtcgctg atggcgaagg agaagaaaat tcaggaaatg
    19021 gataaaggag ttatttagaa atgagatatt tttgaaggaa attttttgga gaagatggtg
    19081 catccgggag gatgactcac tgacgttcgc ccttcgggcc gttgctggcg caacgttatc
    19141 cttcctggtg cctgcggtta ataccgctat ctcttcgata ccttcattgc tgaaggttac
    19201 ggagaatatg gtgcatccgg gaggattcga acctccgacc gctcggttcg tagccgagta
    19261 ctctatccag ctgagctacg gatgcatcgg gaaaacttat tctactgctg atattgatac
    19321 cgctactaaa gccatatcaa ttaagagatg gtgcatccgg gaggattact cgccttgcgg
    19381 ctcgcccttc gggccgttgc taaagcaacg ttatcatccc tggtgcctgc ggttaattcc
    19441 gctacctctt cgataccttc attgctgaag atttcggaga atatggtgca tccgggagga
    19501 ttcgaacctc cgaccgctcg gttcgtagcc gagtactcta tccagctgag ctacggatgc
    19561 atcgggaaaa tttattttac tgctgatatt gataccgcta ctaaagccat atcaagtaag
    19621 agatggtgca tccgggagga ttcgaacctc cgaccgctcg gttcgtagcc gagtactcta
    19681 tccagctgag ctacggatgc aaatggcggt gaggcgggga ttcgaacccc ggatgcagct
    19741 tttgaccgca tactccctta gcaggggagc gccttcagcc tctcggccac ctcaccacac
    19801 gcctcttacg agtgcttcga agaacttgtt tatcgctcat cgtcgctgcg tggcgcacat
    19861 attactttct gagacttata agtcaaacaa tttttcccac acttttatcg tttgcacact
    19921 tcacgctcaa ttagtctgta aaaacgtcaa aaagagtgtt ttatctacag aaaaacggag
    19981 gtctggcaga tagcagtaat gcaaaaaaat ggagacttaa gttgaatgaa cgggagtaaa
    20041 gcgaaaagac tatagagtga aggagaaatt ttgagggtgc gtctcaccga taaagatgag
    20101 acgcggaaag attagtaact ggactgctgg gatttttcag cctggatacg ctggtagatc
    20161 tcttcacggt gaacagaaac ttccttcggg gcatttacgc caatacgtac ctggttgccc
    20221 tttaccccta aaactgtcac ggtgacctca tccccaatca tgagggtctc accaactcga
    20281 cgagtcagaa tcagcattct ttgctccttg aaagattaaa agagtcgggt ctctctgtat
    20341 cccggcatta tccatcatat aacgccaaaa agtaagcaat gacaaacaca ttacatgtaa
    20401 gcagtcatgg cattacattc tgttaaacct aagtttagcc gatatacaca acttcaacct
    20461 gactttatcg ttgtcgatag cgttaatgcg aatgccgtga agcgagtcca cggcattgcc
    20521 tgacgcttat attattgcaa tttcgcgctg acccagcctt tcacactggc taacgctgca
    20581 ggtaaggccg cagcatccgt accaccggct tgcgccatgt caggacgtcc accacccttg
    20641 ccgcccacct gctgagcgac cataccaatc agttcccctg ctttcacacg atctgtgacg
    20701 tccttagata cgcctgcaat cagagaaacc ttaccttcgg ctaccgttgc cagcacgata
    20761 attgtcgacc ccagctgatt ttttaaatcg tcaaccatgg tacgcaacat tttcggctca
    20821 acaccgctaa gctcgctaac caacagctta acaccattaa catcaattgc cttactggaa
    20881 agatttgcgc tctcctgtgc ggcagcttgt tctttaagct gttgtagttc tttttctaac
    20941 tgacgcgtac gttccagtac tgagcgcact ttatccgcca gattattgct atcgcctttc
    21001 agcagatgcg cgacttcgct taagcgatcg ctgtctgcat gaacggtggt gatagcgcct
    21061 tctccggtta ccgcttcgat acgacgaacg cctgcagcag tacccgattc agagatgatg
    21121 cggaacagac caatatcacc agtgcggctg gcgtgagtac cgccacacag ctcggtggag
    21181 aaatcgccca tgctcagcac gcgtacacgc tcatcatact tctcgccgaa cagcgccatc
    21241 gcacctttcg ctttcgccgc ttcgagatcc atgatgttgg tttcgatcgg caaattgcga
    21301 cgaatctgtg cgttcaccag gtcttcgacc gcacgaatct cttccggttt catcgcttcg
    21361 ttgtgtgaga agtcgaagcg cagcaccttg tcgttaacca gtgaaccttt ctgcgataca
    21421 tgagtcccca gaacctggcg cagcgcagcg tgcatcaggt gcgttgcgga gtgattcaga
    21481 cgaatacggg cgcgacgagc ctcatcaaca tcagcctgca cagcgtcgcc cactttcaga
    21541 gaacccgcag caagtttacc gatgtgacca atcgcctggc cgtatttctg cgtatcttcc
    21601 accgcgaagg agaagttagc gcctttcagt tcgcctttat caccaacctg accgccggat
    21661 tccgcataga atggcgtttg atccagcacg accacagctt cctggcctgc attgatggca
    21721 tcaaccgctt taccatcaac aaacagcgcg gtcactttgc cgttcagttc cagatggtca
    21781 tagcctttaa attcagatgc actatcaaca cggatcattg cgttgtaatc ggcaccaaag
    21841 ccgctggctt cgcgcgcgcg acgacgctgc tcttccattg ctgcttcaaa accagcttcg
    21901 tcaactttga tgttgcgctc acgacaaaca tcagccgtca ggtcaaccgg gaagccatag
    21961 gtgtcgtaca gacggaaagc cgtttcacca tccagcgtat caccagaaag ttttgccagc
    22021 tcttcatcca gcaacgccag accgcgctcc agagtacggg caaactgctc ttcttcagtc
    22081 ttcagcacct gctcaacctg cgcctgctgg cgtttcaggt cttcacccgc agagcccata
    22141 acgtcgatca gcggaccaac cagtttgtag aagaaggttt ctttcgcgcc gagcatattg
    22201 ccgtgacgca ctgcgcgacg aatgatacga cgcagtacat aaccacggtt ttcattggac
    22261 ggcattacgc catccgcgat caggaacgca caagaacgaa tgtggtcagc gattacgcgc
    22321 agcgatttat tgctcagatc ggtcgcgcca gtgactttcg ctaccgcctg gatcaacgtg
    22381 cggaacaggt cgatgtcata gttagagtta acgtgttgca gcaccgcagc aatacgctcc
    22441 agacccatac cggtatctac agacggcttc ggcagcggtt ccatcgtgcc atctgcctgg
    22501 cggttgaact gcatgaagac gatgttccag atctcaatgt agcggtcgcc gtcttcttcc
    22561 ggacttcccg gagggccacc ccaaatgtgg tcgccgtgat cgtagaagat ttcggtgcac
    22621 gggccgcacg gaccagtgtc acccatctgc cagaagttgt cagatgcgta tggcgcacct
    22681 ttgttatcgc cgatgcgaat aatacgttcg cgcgggatcc ctacttcttt ttcccagatt
    22741 tcgtaggctt cgtcgtcgct ttcatagacg gtaacccaca gacgctcttt cggcagggca
    22801 aaccattttt cgctggtcag cagttcccat gcaaactgaa tggcatcgtg tttgaaatag
    22861 tcgccgaagc tgaagttgcc cagcatttcg aagaaggtat ggtgacgcgc ggtgtaaccg
    22921 acgttttcca ggtcgttgtg tttaccaccc gcacgcacgc agcgttggga agtggtagcg
    22981 cgggaataat tacgcttgtc gagcccaagg aacacatcct tgaactggtt catcccggcg
    23041 ttggtaaaca acaaagttgg gtcgttatgg ggtaccaggg agctgctggc aactacctga
    23101 tgtcccttac tatggaaaaa gtcgagaaac gcctgacgga tctcagcggt gctcttgctc
    23161 ataattatcc tgaaatcaag ctaacgaaat atcgccacca gctccagcgt gtcttaaccg
    23221 ccgggctggt aactgaaaag tgggaataag ataagttttc ttgagtggga agtaaaatac
    23281 cgtatgcgtt cagtcggcaa aatttcgcca aatatcctgg atatcttcca tcagatagcc
    23341 acgatagagc agaaaacgct ggatcttaac tttttctgaa aagacagttg gcaaaggttc
    23401 accatatttt cgcgtcgcct gatcgcgcac cagtgcgcac cagtcgatgt cacattcacg
    23461 catcgctttt tctgtcgctt cgcgggaaat acctttctga ttcagttcct gacgaatacg
    23521 tgcaggtcca taacctttgc ggctacggct ggcgataaag cgcgcaacaa atcggctgtc
    23581 atcgagatag ccatgttcat ggcaccaggc aataacgcgc tcgtaatctt ctgccgtagc
    23641 atcaatctct tctgggccat ttttgcccat aatcggtgcc gcgagtttac gtcgcagttc
    23701 ttgctcactg tgatcgcgca ccgccagaat gcgtaccgca cgatccaaca ggcgagcata
    23761 tgccgggcga cgggatgttg attctgtcat ggcatatcct tacaacttaa aaaagcaaaa
    23821 gggccgcaga tgcgacccag gttttgctga taaacagatt taatagtcaa tgagtgtgca
    23881 gccaattcgt tagaggatcg aggtgagagt aatttttcac gccagtgttg gcctgttccc
    23941 ctcaccctaa ccctctcccc aaaggggcga ggggaccgtc cgtgcacaca ttatctatta
    24001 tcatcaataa cgaacgatta aaaatcttcg ttagtttctg ctacgccttc gctgtcatct
    24061 acagagaaat ccggcgttga gttcgggttg ctcagcagca actcacgtac tttcttctcg
    24121 atctctttcg cggtttccgg gttatctttc agccaggcag ttgcattcgc tttaccctga
    24181 ccgatcttct cacctttgta gctgtaccac gcgcctgctt tctcgatcag cttctctttt
    24241 acgcccaggt caaccagttc gccgtagaag ttgatacctt cgccgtagag gatctgaaat
    24301 tcagcctgtt taaacggtgc agcgattttg ttcttcacca ctttcacgcg ggtttcgcta
    24361 cccaccacgt tttcgccctc tttcaccgcg ccgatacgac ggatgtcgag acgaacagag
    24421 gcgtagaatt tcagcgcgtt accaccggta gtggtttccg ggttaccgaa catcacacca
    24481 attttcatac ggatctggtt gatgaagatc agcagcgtgt tggactgctt caggttaccc
    24541 gccagcttac gcatcgcctg gctcatcata cgtgccgcaa ggcccatgtg agagtcgccg
    24601 atttcgcctt cgatttccgc tttcggcgtc agtgccgcca cggagtcaac gacgataacg
    24661 tctactgcgc cagaacgcgc cagggcgtca cagatttcca gtgcctgctc gccggtgtcc
    24721 ggctgggagc acagcaggtt atcgatatcg acgcccagtt tacgtgcgta gattgggtcc
    24781 agcgcgtgtt cagcatcgat aaacgcacag gttttacctt cacgctgcgc tgcggcgatc
    24841 acctgcagcg tcagcgtggt tttaccggaa gattccggtc cgtagatttc gacgatacgg
    24901 cccatcggca gaccacctgc cccaagcgcg atatccagtg aaagcgaacc ggtagagatg
    24961 gtttccacat ccatggaacg gtcttcaccc aggcgcatga tggagccttt accaaattgt
    25021 ttctcaatct ggcccagtgc tgccgccaac gctttctgtt tgttttcgtc gatagccatt
    25081 tttactcctg tcatgccggg taataccgga tagtcaatat gttctgttga agcaattata
    25141 ctgtatactc atacagtatc aagtgttttg tagaaattgt tgccacaagg tctgcaatgc
    25201 atacgcagta gcctgacgac gcaccgcatc acggtcgccg ctgaagcatt cccgccgggt
    25261 aatgccttca ccgcgggcag tggcaaaagc aaaccagacg gtgccgacag gcttctcttc
    25321 actgccgcca tccggcccgg cgataccact aatagacacg gcataatcag cacgagccgc
    25381 tttcagtgca cctatcgcca tttccaccac gacgggttca ctcaccgcgc catgctgcgc
    25441 cagcgtctct tcgcgtacgc cgatcatctg cgctttggct tcgttactgt aggtgacaaa
    25501 tccgcgttca aaccaggcgg agctaccggc aatatcggta atcactttcg ctacccaacc
    25561 accggtacaa gactcggcag ttgttacggt tgcgccacgg gctttcagcg cctgcccaac
    25621 ctgttcgctt aactgcatca gttcactgtc agtcatcaca acctcagtca gtcaaaaatt
    25681 tatgccggac aagatagcac tttcgtcagg aagatggtgt tggcgttgca attttacgag
    25741 gaggattcag aaaaaagctg attagccaga gggaagctca cgcccccctc ttgcaaatag
    25801 ttactgcacg cgcgccagcg ccacggcttg tcctaactgc cagaccgcca ttgcgtaatg
    25861 ggtgctgtgg ttataacggg taatggtgta gaagttcggc agaccgtacc agtactggta
    25921 accggtaccg acatccagac gcagcaggct ggcttgttga tggttgccca gcggctgctg
    25981 tggcgttaaa cctgccgtgg caagctgcga aatgctgtac ctggttttga agccatttgg
    26041 caagcctgga gcctgaccgt ttgccattac cgcgacctga tcgcctttca cccagccgtg
    26101 cgctttgaaa tagttcgcca cactaccgat cgcatcaacc ggatcccaca ggttgatatg
    26161 cccgtcgccg ctgaaatcta ccgcatattg tttgtaagac gacggcataa actgtccgta
    26221 gcccatcgcc ccggcaaagg aacctttcag attgagcgga tcgtcctgct cgtcgcgcgc
    26281 catcaacagg aaggtttcca gctcgccaga gaaatactcc gcgcggcgtg ggtagttaaa
    26341 tgacagcgtt gccagcgcat cgaggatgcg agttttcccc atcacgcgcc cccagcgggt
    26401 ttcaacgccg ataatcccga cgataatttc cggcggtacg ccatacacct gccacgcgcg
    26461 attcaacgca tcttcatact gattccagaa aaccacaccg ttctgcacgt tgtccggcgt
    26521 aataaatttt ttgcgataac gcagccatgc gccgttcgga cctgatggcg gtttcaccga
    26581 tgtggttggt gcctggttat ccatcagccg cagtaccgaa tccagacgct tcgcctggga
    26641 gagaatttcc tgcaactgct gacgatcgaa accgtgtttg ttcaccattc tgtcgatgaa
    26701 ctgctgggca ttcgggttat tagcgaaatc gccgcccatc tgcatcacat tgtgctgagg
    26761 ctcaagcagg aagccgccag acggcgttcc ggtggtcgtt tcagtctcag taggttttgg
    26821 cttgctgcta caggcggcaa gcaacacaaa aaggggaagc aatgttacat aacgacgctt
    26881 gaacatgagg ggtccattta acagattcaa ccaggggcaa gtatggtaaa gcatcacgcc
    26941 ccgcacaagg aagcggtagt cactgcccga tacggacttt acataactca actcattccc
    27001 ctcgctatcc ttttattcaa actttcaaat taaaatattt atctttcatt ttgcgatcaa
    27061 aataacactt ttaaatcttt caatctgatt agattaggtt gccgtttggt aataaaacaa
    27121 taaatcctga aggagagaac aatgatagaa accattactc atggtgcaga gtggtttatc
    27181 gggctgttcc aaaagggcgg agaggtgttt accgggatgg tgaccggcat tcttccgctg
    27241 ttgattagcc tgctggttat catgaacgca ctgattaatt ttatcggtca gcatcgtatt
    27301 gaacgttttg ctcaacgttg cgccggtaac cctgtttccc gttacctgct gctaccgtgc
    27361 attggcacgt ttgtcttttg caatccgatg accttaagcc tgggtcgctt tatgccggaa
    27421 aagtacaaac ccagctacta cgcggcagcc tcttatagct gccactcaat gaatggcctc
    27481 tttccccata tcaaccctgg cgaactgttt gtttatcttg gcattgccag cggtctgaca
    27541 acgctgaacc tgccacttgg cccactggcg gtgagctatc tgctggttgg tctggtcacc
    27601 aacttctttc gcggctgggt gaccgatctg accaccgcca tttttgagaa aaagatgggc
    27661 attcaacttg aacaaaaagt tcacctggca ggagcaacat catgacgcgt attcggatcg
    27721 aaaaaggaac gggtggctgg ggcggcccgc ttgagctgga agccacgccg ggcaaaaaaa
    27781 tcgtctatat caccgccggt acccggcctg cgattgttga caaactggca caacttactg
    27841 gctggcaggc tattgacgga tttaaagaag gtgaacccgc ggaggcggaa attggtgtcg
    27901 cggtaatcga ctgtggcggc acattacgct gcggcatcta tccgaaacga cgtattccca
    27961 ccattaatat ccactcgacg ggcaagtcca gtccgctggc gcagtacatt gtggaagata
    28021 tttatgtctc tggcgtaaaa gaagaaaaca tcactgtagt gggtgatgcg acaccacaac
    28081 cctcttccgt gggccgtgac tatgacacca gcaagaaaat caccgaacaa agcgatggtt
    28141 tactggcgaa ggtgggaatg ggtatgggtt ctgccgttgc cgtgttgttt caatctggtc
    28201 gtgacaccat cgacactgta ttaaaaacca ttctgccgtt tatggcgttc gtctcggcgc
    28261 tcattggcat cattatggct tctggccttg gtgactggat tgcccacggt cttgctccgc
    28321 tggcgagcca tccactgggt ctggtcatgc tggcgctcat ctgctccttc ccgctgcttt
    28381 cacctttcct cggcccaggc gcagttatcg cacaggttat cggcgtattg attggcgtgc
    28441 agattggtct cggcaatatt ccgccgcatc tggctttacc ggcactgttt gccatcaacg
    28501 cgcaggcggc ctgcgacttc atcccggtcg gtttgtcgct ggcggaagcc cgtcaggaca
    28561 cggttcgcgt cggtgtccct tctgtactgg tgagccactt tttaaccggc gcaccgactg
    28621 tactgatcgc ctggtttgtc tccggtttta tctatcaata gaggctgaaa catgaccgtt
    28681 atttatcaga ccaccatcac ccgtatcggc gcgagtgcca ctgacgccct cagcgaccag
    28741 atgctcatca cctttcgtga aggcgcgcca gcggacctcg aagagtattg cttcattcat
    28801 tgccacggcg agttgaaagg tgcactccat cccggtttgc aatttacgct cgggcagcat
    28861 cgctatccgg tgaccgctgt tggcagcgtg gcggaagaca accttcgcga actgggtcat
    28921 gtcaccctgc gcttcgatgg tttaaacgaa gcggaatttc cgggcactgt ccatgtggca
    28981 ggccctgtac ccgacgatat cgcgccggga tcggttttga agtttgaatc tgttaaggag
    29041 taaaaaatga atcaggttgc cgttgtcatc ggtggtgggc aaaccttagg cgcgttcctg
    29101 tgccacggtc tggctgccga ggggtatcgc gtcgcggttg tcgatattca gagcgacaaa
    29161 gccgcaaatg tggcacaaga aattaacgcc gaatatggtg aaggaacagc gtatagtttt
    29221 ggtgctgacg ccaccagcga gcaaagcgtc ctggcgctct ctcgtggggt agatgaaatc
    29281 tttggtcgcg tggatttgct ggtctacagc gccggaatag caaaagcagc ctttatcagc
    29341 gacttccagc tcggcgattt tgaccgtgaa tctggtgggt tatttcctgt gtgcccgtga
    29401 attttctcgt ttgatgatcc gcgacgggat tcagggtcgc attattcaga tcaactcgaa
    29461 atccggcaaa gtgggcagca aacacaactc tggctacagc gcagcgaaat ttggtggcgt
    29521 cgggctgact caatcactgg cgctggatct ggcggagtac ggcattacgg tgcattcact
    29581 gatgctcggt aacctgctga aatcgcctat gttccagtca ctgttacccc aatacgcgac
    29641 caagctgggt atcaaaccgg atcaagtcga gcagtattac atcgacaaag taccgctcaa
    29701 acgcggctgc gattatcagg atgtgctgaa tatgctgctg ctctatgcca gtcctaaggc
    29761 gtcgtactgc accggacagt cgatcaatgt caccggcggt caggtgatgt tctaatcaac
    29821 agcggagatc cgttaaggat ctccgtgaga ctaatagaat gcctgatgcg ctacgctcat
    29881 caggcataca ggacttccgc cactacatta aggaaaagtt atggtatccg cactcatcac
    29941 cgtcgccgtt atcgcctggt gtgcgcaact ggccttaggc ggctggcaaa tttctcgttt
    30001 taaccgtgcc ttcgacacac tatgccagca agggcgggtt ggcgtgggcc gttccagcgg
    30061 gcgctttaaa ccacgggtcg tggtcgccat cgccctggac gatcagcagc gcatcgtcga
    30121 caccttgttt atgaaaggac tgaccgtctt cgcacgaccg caaaaaattc ccgcaattac
    30181 cggtatgcat gcgggtgatt tacagcccga tgtgatcttt ccccatgatc cactatcaca
    30241 gaatgctcta tcattggcgc ttaaactgaa acgtggataa tttcgttgtg aatgttactt
    30301 gcttgcgaag ttatcatttt gaaacctaaa tcaggtaatc acgcccatga aacctcgtca
    30361 gcgtcaggcc gccattctgg agtatctgca aaagcagggt aaatgctcgg ttgaagaatt
    30421 ggcgcaatac tttgacacca caggcacaac cattcgcaaa gatctggtca ttctggaaca
    30481 tgcaggaacc gtcattcgta cttatggcgg agtggtgttg aataaagagg aatccgatcc
    30541 gcctatcgat cataaaacac tcatcaacac ccacaagaaa gagctgatag cagaagctgc
    30601 cgttagtttt attcatgatg gcgattcgat cattcttgat gctggcagta ccgttttgca
    30661 gatggttccc ctgctctcgc gctttaataa catcacggtg atgaccaaca gtctgcatat
    30721 cgtcaatgcg ctatctgaac tggataacga acaaactatc ctgatgccag gcggaacgtt
    30781 tcgtaaaaaa tcggcctcat ttcacggaca gctggcagag aatgccttcg aacatttcag
    30841 tttcgataaa ttgtttatgg gcaccgacgg catcgatctc aatgcgggcg taaccacctt
    30901 taacgaggtt tataccgtca gtaaggcaat gtgcaatgcc gcgcgcgaag tgattttgat
    30961 ggcggactca tcaaagtttg gccgtaaaag ccccaacgta gtttgcagtc ttgaaagcgt
    31021 cgataagctg attaccgacg caggtatcga tccggcgttt cgtcaggcgc tggaagagaa
    31081 agggatcgat gtgatcataa ccggagagag caatgagtga agcactactg aacgcgggac
    31141 gtcagacgtt aatgctggag ttgcaggaag caagccgttt accggaacgt ctgggcgatg
    31201 attttgttcg cgccgccaat atcatcctgc actgcgaagg caaagtggtg gtttcgggaa
    31261 ttggcaaatc gggccacatt ggtaagaaaa tcgccgcaac acttgccagt accggcacat
    31321 cggctttttt tgtccatccg gcagaagcac tgcacggcga tctggggatg attgaaagcc
    31381 gcgatgtgat gctgtttatc tcttactccg gtggcgcgaa ggaactggat ctgattattc
    31441 cgcgtctgga agataaatct atcgcgctgc tggcgatgac cggcaaaccg acgtcaccgc
    31501 tgggcctggc ggcaaaagcg gtgctggata tctccgtaga acgcgaagcc tgcccgatgc
    31561 accttgcgcc gacctccagc accgtcaata ccctgatgat gggtgacgcg ctggcgatgg
    31621 cggtcatgca ggcgcgcgga tttaatgaag aagattttgc ccgctcccac ccagccgggg
    31681 cactgggcgc tcgcttgctg aataaagtgc atcatctgat gcgccgtgac gatgccatcc
    31741 cacaggtggc gttaaccgcc agcgtgatgg atgcgatgct ggaactcagc cgcaccggtc
    31801 tggggctggt ggcggtatgt gacgctcaac aacaggtaca aggcgtcttt accgacggcg
    31861 atttacgtcg ctggctggta ggcggcggcg cactcaccac gccagttaat gaagcgatga
    31921 cgacaggcgg caccacgtta caggcgcaaa gtcgcgccat cgacgccaaa gagatcctga
    31981 tgaagcgcaa aatcactgcc gcaccggtgg tggatgaaaa cggcaaactc accggcgcaa
    32041 ttaacctgca ggatttctat caggccggga ttatttaatc cttcattccc agacgtttcg
    32101 ccagccgatg caggttggcg acgtcggttt ccagcatccg cgcgcaggca gcccagttat
    32161 gatgattttg tgccagtgcc tggcgaatag tttcacgctg gaacgcttct gtcgcttcac
    32221 gcaggttttg cttaacaacg ggcaccgccg ccgcttctgg cggcggcaac gtcacctcag
    32281 gaaaagcaaa atgttgcgcc tcaagaatca cttcatcgcc gttgcgggtg gctctcgcca
    32341 gcactaccgc ccgatgaata gcatgttcca gttcgcgcac gttccccgga aaacgatagt
    32401 gttgcagtaa atttcgcgct ccggcactta ataccacgcg ggagagcccc agccgcaaac
    32461 gacactgctc gcagaaatac cccgccagca gaatgacatc atcgccccgc tcacgcagcg
    32521 gcggcaccga aagtggaaac acgctcaggc gatgaaacaa gtcagcgcga aatcgccctg
    32581 ccatcacctc ttcgcgtaag tcgcggttag tcgccgccag cacgcgcaca tcgacccgca
    32641 aactacggtc atcgccaacg cgctgaatat cgccatactg caacaccctc agcagcttgg
    32701 cctgcaatgc caacgacaac tcgccgatct catccagaaa cagagtgccg ttatccgcca
    32761 tttcaaactt cccgctgcgg ttactgatag cgccagtaaa cgctcctttc acatgcccga
    32821 acaactcact ttccgccaca ctttccggca gtgcagcaca gttgagatag accagcggat
    32881 tcaccgcccg tggcgaggct tcatgaatcg ctttcgccac cagctcctta ccggttcccg
    32941 tctcaccgct gatcaggacg ttgagatcgg acgccgccac aatctcaatc tcttttttca
    33001 gttgcgtcat gccaggggac aagccaatca tctgcgtctg tttcaccgct tcaaacggcg
    33061 tggcatcgcc tggcatcata ttctggcttt ccagttgttc aatcagcaac gcattgctta
    33121 acgctcccgc cgccagcgca gcaatcagcc gtagctcttc gtcgctgaaa acatcgaact
    33181 gatcgggctg catcccgtcg agcgtcagtg cgccgatcag gttttgcccg gcaaacaatg
    33241 gcagaccaac gcaggcgtga accttcagac tctcctgccc aggaatcaaa ccgtcatagg
    33301 gatcgggcaa ttcgctgtct gcgggaaagc gcaccacatc cccggcgcgg gcaatcgctt
    33361 ccagccgtgg atgcccttcc agcgcaaagc gtctaccgag tacatccttt gccagaccgt
    33421 cgatggcaag cggaataaac tgccgcgaat cgtaacgtag caacgcagac gcatcgcact
    33481 ccagcacctg acgtagcgtg gtgatcaggc gctgaaaacg atcctgatga ccaatcccac
    33541 gctgcaattc gatggcgata ttcgccagca catcaacgga aaaactcatc tttgcctcac
    33601 tgtcaatttg actatagata ttgtcatatc gaccatttga ttgatagtca ttttgactac
    33661 tcattaatga gcataatttt atttatagag taaaaacaat cagataaaaa actggcacgt
    33721 aatctgcaat tagcaagaca tctttttaga acacgctgaa taaattgagg ttgctatgtc
    33781 tattgtggtg aaaaataaca ttcattgggt tggtcaacgt gactgggaag tgcgtgattt
    33841 tcacggtacg gaatataaaa cgctacgcgg cagcagttac aacagctacc tcatccgcga
    33901 agaaaaaaac gtgctgatcg acaccgtcga ccataaattc agccgcgaat ttgtgcagaa
    33961 cctgcgtaat gaaatcgatc tggcggatat cgattacatc gtgattaacc atgcagaaga
    34021 ggaccacgct ggggcgctga ccgaactgat ggcgcaaatt cccgatacgc cgatttactg
    34081 tactgccaac gctatcgact cgataaatgg tcatcatcat catccggagt ggaattttaa
    34141 tgtggtgaaa actggtgaca cgctggatat cggcaacggc aaacagctca tttttgtcga
    34201 aacgccaatg ctgcactggc cggacagcat gatgacttac ctgacaggcg acgcggtgct
    34261 gttcagtaac gatgctttcg gtcagcacta ctgcgacgag catctgttca acgatgaagt
    34321 ggatcagacg gagcttttcg agcagtgcca gcgttactac gccaatatcc tgacgccgtt
    34381 cagccgcctg gtaacaccga aaattaccga gatcctgggc tttaacttac cagtcgatat
    34441 gatagcgacc tctcacggcg tggtatggcg cgataacccg acgcaaattg tcgagctgta
    34501 cctgaaatgg gcggctgatt atcaggaaga cagaatcacc attttctacg acaccatgtc
    34561 gaataacacc cgcatgatgg ctgacgctat cgcccagggg attgcggaaa ccgacccacg
    34621 cgtggcggtg aaaattttca acgtcgcccg aagcgataaa aacgaaatcc tgaccaatgt
    34681 cttccgctca aaaggcgtgc tggtcggcac ttcgacgatg aataacgtga tgatgccgaa
    34741 aatcgccggt ctggtggagg agatgaccgg attacgcttc cgtaacaaac gcgccagtgc
    34801 tttcggctct cacggctgga gcggcggtgc ggtggatcgt ctttccacgc gcctgcagga
    34861 tgcgggtttc gaaatgtcgc ttagcctgaa agccaaatgg cgaccagacc aggacgctct
    34921 ggagttatgc cgtgaacacg gtcgcgaaat cgcccgtcag tgggcgctcg cgccgctgcc
    34981 gcagagcacg gtgaatacgg tggttaaaga agaaacctct gccaccacga agtccgacct
    35041 cggcccacgg atgcagtgca gcgtctgcca gtggatttac gatccggcaa aaggcgagcc
    35101 aatgcaggac gttgcgccag gaacgccgtg gagtgaagtt ccggataact tcctctgccc
    35161 ggaatgctct ctcggcaaag acgtctttga cgaactggca tcggaggcaa aatgagcaac
    35221 ggcattgtga tcattggttc gggcttcgcc gcccgccaac tggtgaaaaa tattcgcaaa
    35281 caggacgcca gtattccatt aaccctgatc gccgccgaca gcatggatga gtacaacaaa
    35341 ccggacctca gccatgttat cagccagggg caacgtgccg acgaccttac ccgccagacg
    35401 gcgggtgaat ttgccgggca gtttaatctg cacctgtttc cgcacacctg ggtgacggat
    35461 atcgatgccg aagcccatgt ggtgaaaagc cagaataatc agtggcaata cgacaagctg
    35521 gtgctggcaa ctggtgccag tgcctttgtc ccgccagtgc ctgggcgtga gttaatgctg
    35581 acgttaaata gtcagcaaga gtatcgcgcc tgtgaaacgc aactgcggga tgcccgacgc
    35641 gtgttgattg tcggcggtgg tttgattggt agcgaactgg cgatggattt ttgtcgtgca
    35701 ggcaaagcgg tcacgctaat cgacaacgct gccagtattc tggcgtcatt aatgccacca
    35761 gaagtaagca gccgcttgca gcatcggttg acggagatgg gcgttcatct gctgttgaaa
    35821 tctcagttgc aggggctgga aaaaacggat tctggcattc tggcaacgct ggaccaccag
    35881 cgtagtatcg aagtggatgc ggtaattgcc gccaccggac tgcgcccgga aacagccctg
    35941 gcacgacgcg ccgggctgac gattaatcgc ggcgtttgcg tcgatagtta tctgcaaacc
    36001 agtaatgacg atatttatgc gctgggcgat tgcgcggaaa tcaacggtca ggtactgcca
    36061 ttcctgcagc cgattcaact tagcgcgatg gtgctggcaa aaaatcttct cggcaataac
    36121 acgccgctga aactaccggc gatgctggtg aaaatcaaaa cgccggaatt accgctgcat
    36181 ctggcaggcg aaacccagcg tcaggattta cgctggcaaa ttaataccga acgccaggga
    36241 atggtggctc gcggtgttga cgatgctgac cagcttcgcg cctttgtggt cagtgaggat
    36301 cggatgaaag aggcatttgg attgttgaaa acattgccga tgtaggtggg ctactgtgcc
    36361 taaaatgtcg gatgcgacgc tggcgcgtct tatccgacct acggggacgc atgtgtaggc
    36421 cggataaggc gtttacgccg catccggcaa tggtgtccaa atgcaacacg ttttatccgt
    36481 tctggacttc acccgctaac caacgcgccg cagcaataac cccctgcccc agagacaaac
    36541 cgccatcacc cgccggtaaa ctctgtggaa agagcaatgt gaaatcagcg agataatgcg
    36601 ccagacgtgc acgcagcaaa cggttatgaa taaccccgcc gctaaatacc agcgtagtga
    36661 taccacgcat cgtggcctgc tcacgcatca acgcggcaaa accctgcgcc agcgcatcat
    36721 gaaacgccca cgcgcgttga ttaaccggtg cctgccagtt cagccactgc tgccagaaag
    36781 cggcgagatc cagttgattg tccaccagcg gcatcgtcac cggatgcgtc actccgtggc
    36841 atgaggctgc gagcgcctcc agagcacaag ccgcttcacc ttcataactt aacgtggctg
    36901 gcgcacagcc cagtgccgcc gccactgcat cgaacaaccg cccacacgat gacgccagcg
    36961 gcgcgttaat tccacgctca atggcccgcg ccagcacgct ccagttttgc tgttgcacac
    37021 ttgctgtttc agggtaattc tgccactccg gcacaaagcg caggcactgc gccagcaggt
    37081 ttcgccacgg ctgcttcgct gccaaatcgc cacccggaag cgccactgca ggcaagccgc
    37141 ccaggtgctc acattcgcga tagttcaccc gcaggcactc gccgccccac aaagcgccgt
    37201 tctcccccat accgataccg tcgagcgtca aagcaatgac atcaccgcca tccaacggcc
    37261 actgatgctc tgccagacac gccgctgcat gggcatgatg atgcagtacc gtttgcgtcg
    37321 gcagattcat ttcacgcgcc cactggctgg agacatagcc cggatgcgcg tcatgcacaa
    37381 cgtattgcgg ggtaaaatcg tagatgtttt gcatcaggcg taacgcttcg cgccactgca
    37441 tctggatgcc atcgtcactt aaatcgccca gatgctgact caacaccgct tgttcaccgc
    37501 gcaccaggca gaaggtattt ttcagatccg cgccgagaca cagcacaggc ggaacatttt
    37561 taaagcccgg aggcaaagcc agcgcatccg gcacataccc ccgcgaacgg cgcagcattt
    37621 cgccgctttc gcgcaccacc gaatcatcca tccgctgcac gatgtcgcgg ttatgtatca
    37681 agaatccgtc ggcaatgccc tgcaaatccg ccagcgcctg ttcgttgctg atagctggtg
    37741 gtttaccgct caggttgccg gaggtcatca ccagcgggca ttgcagttcc tgtaacagca
    37801 aatgctggag cgggttcgca ggcaacatta ccccgacttc gttaaggtca ggggcgatat
    37861 catcacaaag ctcaggaacg tattttttat ccaccagcac aatcggcgcg gcgggcgtgg
    37921 taagcaactg gcgcgcagcg tctggtaaac cgtcagccac tggcaacatg accgccagcg
    37981 gtttcgccgg gcgatgtttg cgcgcccgaa gtgtcgccac cgcgttactg ttacgtgcat
    38041 cgcaggcaag atgaaatccg ccaatccctt tgatggcgac aatgttgccc atttttaact
    38101 gtgcgatagc cgcctgtaat gccgcctctt gttctgcatg ttcaccatga cttacccatt
    38161 caagatgtgg gccacactcc gggcaggcca ccggctgggc gtggaagcga cgatcgagcg
    38221 ggtcacggta ctctttgtca caagccggac acaacggaaa cgccgccatc acggtaaacg
    38281 ggcggtcgta aggcatggcg cgaataatgg tgaaacgcgg gccgcagtgg gtacagttga
    38341 taaacggata acgataacgc cgttcgcctg gggtattcat ttcggcaagg caagcagggc
    38401 aagtagcggc atccgggaca atttgcgtat tcatggcacc tcccgcgctc tggctgatgg
    38461 tgaactcagt gggcagttgt gaccagatgt acggctcacg ctcgacgcta tcaatacgcg
    38521 ccagcggcgg gcagtgctga tgcaattgaa caagtaacgt ttccgggtct tcccgcagcc
    38581 ggacttctac gccatcgccg tcattacaga catcgccgtg aagatttaat tgctgtgcca
    38641 gctgccagac aaacggacga aaaccgacgc cctgcacttt gccacgaata cgcagttgga
    38701 caccgcaaga tgtgtttttt gccattgagt tattcccgcc atcatgaatt gcgtaacccg
    38761 tcctgccgga cacgactgcg tcgtatccgg cagtctcagg tcggcgatac cgccagctcc
    38821 gtattctacg aatatttccg ggaattcctt tgatgccaga acagttctgt aagattttta
    38881 gaacatcagc gccgtacggc ggcgtttttc tgcactcagt tgttcaagtt tattacgatc
    38941 gacacaaatc agcgcatgag tcgggcaagc cgccatacac gccgggccgt cttcacgatg
    39001 gttgcacagg tcacatttat tggcttcggc tttgtcagcc cgtacattca gccccgcgcc
    39061 gctgttgcga atcaccggac gtaccaccac ttccatcgcg ccatacgggc aagccacaac
    39121 gcaggttttg caaccaatgc aacgttcctg catcacatga acaaaccctt tatcacggct
    39181 gatagcacca ttcgggcaga cgttagcgca cggtgcatct tcacactgac ggcaaactgt
    39241 cgccgtggaa atgttcacac ctttaatgac atggatacgc ggtaaaaatg tttccggggt
    39301 cagcgatgca cagtcctgat tttcctgatg agaaaccacg cacgccactt cacaggtacg
    39361 gcaaccaata catttactcg cgtcagcaat gatgaaacgg ttcatcaaat tctccagcaa
    39421 tgacagttaa tgcgccgata cattcacaaa tcatgccagt tttaattcac tgttatttaa
    39481 ggaaattaat ttctgtaatg caggaaaaaa gatgtcatcg acactagtga cgatgacatg
    39541 tgatgacaat gtttatcgcg aaagagcaat gagtgagtcg cggcggatca gttttccgct
    39601 gaaggttttc ggcggtgaga aatccccgcc atcgagcata aaaatcagcc gtccaataat
    39661 ttcctgaatc atctcagtca ccggaatttt tacgctggag agcgccggaa cgatataggg
    39721 ggcaattgcg atatcatcga atccgataac tgacacctgc tctggcaccg ctacgccgcg
    39781 ctcgtgcaac gctttcatcg cacctatcgc catatcgtcg ttactggcaa ctaacgcgct
    39841 aaatttagcc ccacgttcga gcaacgtttc taccccttcg gccccgctgg cgggcgtcca
    39901 tttaccgtta gcgataagtt tttcattgag cgcaatacca tgctgcgcca gcgcgtcttt
    39961 atacccagca agacgttcaa tgctggtggg ggaatccatc gagccggtaa ggaaagcaat
    40021 ctcctgatgt ccggcgttta tcaactctgc cacggcatta aagctggtct gtttatgatc
    40081 gcaccagacg ctatggctgc tgtttttgcg caggcggcga ttaagcacca ttatcggctg
    40141 actgtgcgcg tcaatgatgt catcgatctc atccacgctt aaaaaacgcg ggtaaatcat
    40201 aatggcgtcg cagcgcaaat ccagcagata ctgaatcgcc tggcgctctt cttcggcgct
    40261 gtgtttacca tctgccaata gcaactgccg ccctttctct tccgccattc gcgcggcatg
    40321 aaagagtaat tcactaaaat aaatgccgtg gtaaagcgtg ttggtcacta ccaaccccag
    40381 cgtctgagta ctcttcgccg acagattgcg cgccagcaag tttgggcggt aaccgctctc
    40441 ttctaccgcc tgaaacacgc gatctttagt ctcctggctg acgtagccat tacctgaaag
    40501 cacgcgggaa acggtcgctt ttgaaacccc ggcgcgcttc gccacttcca gcatcgtcgt
    40561 catcattttc atccctttac acgcaatcaa cgcagtgtac tgcaccgttt gccgattgtc
    40621 cttgcgcaat cggcgggaaa aaaattcagg tgaccggttt cacaaatata aaaaatgaac
    40681 aattaactct cttgcttatt tagtgacaac tattcatgat tttgtgaaac cggtttccta
    40741 attccgtttc agcatcggca tttttccgtt acgtcgactg ataacaacta catctaccct
    40801 actgataaca ggataaaatc cgatggccaa aaattatgcg gcgctggcac gctcggtgat
    40861 agcggcactg ggcggcgttg ataacatctc ggcggtcacg cactgtatga cgcgtttgcg
    40921 cgttgttatc aaagatgacc agcttatcga cagcccgacg ttaaaaacca tccccggcgt
    40981 gctcggcgtg gtacgtagtg acaaccagtg tcaggtgatt atcggcaata ccgtttcaca
    41041 agcctttcag gaagtcgtca gcctgctgcc gggagatatg cagcccgcac agcccgtggg
    41101 taaacccaaa ctcacgctgc gtcgcattgg tgcggggatc ctcgatgcgc tgatcggcac
    41161 catgtcaccg ctgatcccgg cgattatcgg cggatcgatg gtcaaactgc tggcgatgat
    41221 cctcgagatg agcggcgtgc tgacaaaagg atcgccgacc ttaaccattc tgaatgtgat
    41281 tggtgacggt gctttcttct tcctgccgct gatggtcgcg gcatctgccg ccatcaaatt
    41341 taaaaccaat atgtcgctgg cgattgccat cgcgggtgtg ctggtacatc cgagttttat
    41401 tgaactgatg gcgaaagcgg cccagggtga acatgtcgaa tttgccctga ttccggtcac
    41461 cgcggtgaaa tacacctaca cagtgatccc ggcgctggtg atgacctggt gcctgtcata
    41521 tatcgaacgc tgggtggatc gcattacacc ggcggtgacc aaaaacttcc tcaagccaat
    41581 gctgattgtg ttgattgccg caccgctggc aatcctgctg attggcccga ttggtatctg
    41641 gatcggtagc gccatttcgg cgctggttta caccattcat ggttatctgg gctggctttc
    41701 agtcgccatt atgggcgcgc tgtggcctct gctggtaatg accgggatgc accgcgtctt
    41761 tacgccaacc atcattcaga ccattgccga aaccggcaaa gaagggatgg tcatgccgtc
    41821 agagatcggc gctaacctgt cgctgggcgg ttcatcactg gcggtggcgt ggaaaacgaa
    41881 aaacccggaa ctgcgccaga cggcgctggc tgcggcggca tcagccatta tggcggggat
    41941 ttccgaaccg gcgttatacg gcgtggcgat ccgcctgaaa cgtccgctta tcgccagtct
    42001 tatcagcggt tttatttgcg gcgcggttgc cggtatggcg gggcttgcca gccactcaat
    42061 ggcagcgcca gggttattta ccagcgtgca attcttcgat ccggcgaatc caatgagcat
    42121 cgtctgggtg ttcgcggtca tggcgctggc ggtggtgctg tcgtttatcc tcacactgtt
    42181 gctcggcttt gaggatattc ctgttgagga agcggctgcc gaggcgcgaa agcatcagag
    42241 cgtacaaccg accgtcgcca aagaagtaag tcttaattga ggatgaaaat gtcagtattt
    42301 ccagaaggtt ttttatgggg cggcgcgctt gccgccaacc agtctgaagg tgcgttccgt
    42361 gaaggtggca aagggctgac cactgtcgat atgatcccac acggcgagca tcgaatggcg
    42421 gtgaaactgg ggctggaaaa acgttttcag ttgcgcgatg acgagtttta tcccagccat
    42481 gaggcgacgg atttttatca tcgttataaa gaagatatcg ccctgatggc agagatggga
    42541 ttcaaggttt tccgtacctc aattgcctgg agccgtctct ttccgcaggg cgatgaaatc
    42601 acgcccaatc agcagggcat tgctttttat cgttctgtct ttgaagagtg taaaaagtac
    42661 ggtatcgaac cgctggtcac gttgtgccac ttcgatgtgc cgatgcatct ggtcaccgaa
    42721 tatggctcct ggcgtaaccg caagctggtg gagtttttca gccgctacgc ccggacctgc
    42781 tttgaagcat ttgatggtct ggtgaaatac tggcttacct tcaatgaaat caacattatg
    42841 ttgcatagcc cgttctccgg cgcgggtctg gtgtttgaag aaggtgaaaa tcaggatcag
    42901 gtgaaatatc aggccgcgca tcaccagctg gttgccagtg cgctagccac caaaatcgcc
    42961 catgaggtta acccgcaaaa tcaggtgggc tgtatgctgg cgggcggtaa cttctaccct
    43021 tacagttgca agccggaaga tgtctgggcg gcgctggaga aagaccggga aaacctgttt
    43081 tttatcgatg tgcaggcgcg gggcacgtat ccggcttact ctgcccgcgt attccgcgaa
    43141 aaaggggtaa ccatcgacaa agcaccgggc gatgatgaaa tcctgaaaaa caccgtcgat
    43201 tttgtctctt tcagctatta cgcctcgcgc tgcgcctcgg cggagatgaa cgccaacaat
    43261 agcagtgcgg cgaacgtagt gaaatcgctg cgtaacccgt atctgcaagt gagcgactgg
    43321 ggctggggta ttgatccact cggtctgcgt atcaccatga acatgatgta cgaccgttat
    43381 cagaagccgc tgtttctggt ggaaaacggc ctcggcgcaa aagatgaact tgctgccaat
    43441 ggcgagatta acgatgacta tcgcatcagc tatttacgcg aacatatccg cgcaatgggc
    43501 gaagcgattg cagatggcat tccgctgatg ggctacacca cctggggctg tattgattta
    43561 gtctccgcct ctacgggtga aatgagcaaa cgctacggtt ttgtctacgt agaccgtgac
    43621 gacgcaggca acggcacgct gacgcgcaca cgtaagaaat cgttctggtg gtataaaaaa
    43681 gtgattgcca gtaatgggga agatttagag taggtaacag tgccggatgc ggcgtgaacg
    43741 ccttatccgg cctacatgtc gcacgaacca ggaaggcccg ataagcgcag cgcatcgggc
    43801 aatctggggt ttggcatcag tcaaaatcaa agaaaccatt ccggcttaac gccaaatcgg
    43861 gctgaaagtg ccttaatgtg ggatatcgtc agggatcgtt gtccagacaa aatctgactg
    43921 accagggatt tagagccgat ttcgttcttc aaatccgcat aggtcagatt atgttgatca
    43981 atcagcgtgc gaagtaacgc cacgccaaca ggcattgccg caacggcttt gtcgaactct
    44041 gcaaactctt ggttgttgtt ttcatattct gcaatgcggc tcgccagaaa gtcgataagt
    44101 ggattggtgt catcttcttc aatcaaatat tcgaccagtt ccagtgcttc acgataatca
    44161 tcttctgagt cactacctcc cagaaagggg actgcattga ccagttctct tgttgcttta
    44221 acagcgcgtg cagcattagc agtcattctt tgttctctct gtagtaacgt gtcagtttgt
    44281 catactcggc gtgattagtt atgtgcttca caaagaaccg cttattaaca aagtttatgt
    44341 aggcaatcac ccttaggttg ttcccaccta catccagaac gtaccatttg ttgcgatatt
    44401 taaaattgtc gaggttggga aacgccgttc gcatctcttc tggcgtagaa aaatccgttt
    44461 ccttaataac ccgatacaat gcctgaagag ccagagcgtc gttaggatat ttacgcgcag
    44521 actcttcaaa tggcgcttta gaaatgatgt gcatttttaa gttttaactt ccttatagta
    44581 agtaaacagc cacatctgcg agttctatcc ttatgtgttc ggaaactata gctcatcaaa
    44641 tccaagttca caatatgtaa acacttgagc gcgatataat tacccccaaa atcctcatag
    44701 ccactctgca aaaagtcagt tctggttcac cccatcaaga acatccccct ttatcaagca
    44761 acgctgcgtc tgcgccagat cttccgtttg cgggtcgcgc ccggcgatgg gtttgaggac
    44821 gagtttacgg ttgggggtat aagtaaaacg cgtgagcgcg ggatagctta acttgccaaa
    44881 ccagccgtga acacgctgcc atttggcgtc ttttacctga tacagactgg ttatccagta
    44941 accggaatca aactgcatat ccagcaattg cgtgtgacca ttgccttgca aatccagcaa
    45001 atcatctacg ccagttttgc ttgcggtgta gaatcccctg ggttcaaaga cacagggacg
    45061 gccttcgcgc gtgaacgtca taagaattaa atgtgcataa ggagccagac cattaccggt
    45121 acttggtaac cagataacca gatcctgaat gccgttacga tcaagatcgg cgcgatagag
    45181 ttgccctttg cccgctatgt ttgccagcat acccacaggc acagaccagg atttaccggc
    45241 gcgatcttta ccgttcaatt gccattcact atcatcattt ggcatctttt caagcgtcac
    45301 gccagcaacg cccacttttc taaaatgcca ggtttgctcg gcatcataag gcatggtcag
    45361 gagaggtttc agcgcccgct tttgcgcgta ttcgctgtca ctcattggca gatattcggt
    45421 aaagctggtg gcctgtgcag gcagcactaa gctcaataaa aaagcgcaca tccctgttct
    45481 gatattcatt tcgctgaatt ccctgatgga gaattattct tcttccaccg ctaactgcgc
    45541 gaagccgcca ttcccttccc agccttccag tcgttgataa acggtttcta ccgcatcttt
    45601 aatcggctga gtcatcgggt agtaaaagcc gacgatatcc ggctggatgc cgaggaaaat
    45661 cacttcgcca atgtcttctt tcaactggtc gataagatag ttcagcggca tgttatgggt
    45721 agtcatcata aacatctcgg cgatatcatc cgggtcgatg atgcggattt cgccggggtt
    45781 tagccccata tcagtagcgt caacaatcag taaccgcgta gggcgcagtt cacggatggc
    45841 gacgatgtcg ttttccggtg cactaccgcc atcaatcacc acccagttac ctttcggcgc
    45901 ggcggcgcac ttttccgcca gcagcgggcc agcgccatca tcgcccatca tgctattgcc
    45961 aacacagagt aaaacgtcag tcacgcagtc tcctcaccat caaatagatg gcgttttcct
    46021 gatgaatatc atgcagcata cttaacagca ttgtactcca ttccttctgc tgcgaagttt
    46081 gcgctgcccg ggctttatcg aatgcgttgg ccagcatcgg cacatggtta atgtcgatga
    46141 cgatctcacc atatttcggc acgccttcca ttttgcggcg ggcttcactg cccgcctcca
    46201 gagtggcaat ccatgccaga tattcatccc acgggcaggt gagcgccgct tccaggcaat
    46261 cgataacccc aaggtggtga ccaatcgcca ggctgtaata gaccacctgc tgcgcctcgg
    46321 cgggcgtggc atcgttctca tcaataaatt tacggctcag ttgactgaac accacctttt
    46381 cactcatcgg atacgcgcct cttcaacaac atgattcaga tggctgacaa tctcgttcag
    46441 acgcggatcg ttttccgctt ccagccagcg tgcaacctgt tcttcgccct gccctaactg
    46501 tgtcaggaag tcatcggcaa tctgacgacc gtaacgataa cccgccaggc ggcgtgcttc
    46561 gcgatccact ttcacacgca gcggctgcac catatcgcca tgcaggattt ccgcctgttg
    46621 ttcatcctgc tcacccgggc cacgggcgtg aattttctgc tccagcaggc cgagcgccat
    46681 tgcaaagccg tacagcgtgg cggcaggcgt tggcgggcaa ccaggaatgt agacatctac
    46741 cgggacgatt ttatccgtac cgccccacac gcagtagaga tcgtggaaaa taccaccgct
    46801 gttaccgcag gcaccgtagg agatacaaat tttcgggtcc ggcgcggact gccacgcacg
    46861 cagcgcaggg gatcgcattg cacgggtgac cgcgccggta aacagtaaaa tatccgcatg
    46921 acgcggtgaa ggaacgactt taatgccgaa gcgttctgca tcaaacagcg gcgaaagcgt
    46981 ggcgaaaatt tcgatttcgc agccgttgca gccgccgcag tccacgcgat aaacgtaggc
    47041 agaacgtttg atttttttca gtaacgacgc cttcatgctg gcgatggatt catccaccgt
    47101 catcgggacc ggaatgccgt tggcgtcacg ggggcctaat aaattgctca tcagatggcc
    47161 tctttcatat ggcgagtcag ttcaatacgg tcggacggca ccaggcattt ctggcgctta
    47221 cattccgggc aagtctcaaa gctttcgcgg tggttttccg cgcggctgtc gccgttgtgc
    47281 ttaagcagcg caatggcgta gtcgatctct ttctggacgg cgaaaggacg attgcagacg
    47341 cggcagttgc acagcgcgaa gcgggactgt tgcaggaagt cttctttctt ccacactgcc
    47401 agttcgtact cttgcgacag tttgatcgcc gccgtcgggc aaacttcttc gcagcgtcca
    47461 cagaagatgc agcgcccgag attaaactcc cacgccagct caccggtggc gaggtcagtt
    47521 tcaaccgtta aggcgtttga cgggcaggca ttgacgcagg ccgcacagcc gatgcactgc
    47581 tgcgggttct gctccggctt accacggaag tttttatcaa ccgcaatcgg ctccagcgga
    47641 taagacgagg tcgccgtgcc ggttttgatg acttttttga taaaggtaaa catggcgatt
    47701 ccttatttca gcggcgagtt tttacgctca atgctgtaac gctcgagttc tttgtacggc
    47761 accactttgc tctttttctt acgcacatcg accacggtca tgcggtcggt acaggagtag
    47821 caagggtcca agctaccgat aatcagcggc gcatcggaaa cggtgttacc gcgcagcatg
    47881 tagcgcaggg tcggccagtt cgcgtaggtc gcggcacggc agcgccagcg gtacagcttc
    47941 tggttgtcgc cggtcatgct ccagtggata tcgtcgccgc gcggcgcttc ggcaaagccc
    48001 agcgcgaaac ggtgcggaat gtaggtaaag ccttccacca tcagcggacc gcccggcagg
    48061 ttatccagac cgtagtcgat catgttcagc gcggtataaa cttcgttgat acgcactttc
    48121 agacgggaaa taacgtcgca gccctgctcg ctgtggactt ccattggcag caggccataa
    48181 ccgacaaacg ggtgatcggc gcgagtatcg cgggcgtgac cgctggcgcg aaccatcggg
    48241 ccaacgttac tgaaatcgcg ggccatttcc gggtccagac gaccaatgcc gacggtgcgc
    48301 tgttccatgt tcggcgtact cagcagcaca tccaccagct cctgcacttc acgacgcatc
    48361 tgttgcgcca gctggcgggt ctggatcatg tcatctttca acagatcgcg acgaatcccg
    48421 ccgatcaggt tcaggccgta ggttttacgc gcaccggtga ggatctctgc cattttcatg
    48481 gaggtttcac gcacgcggaa gaactgcata aagccggagt caaagccggt aaagtggcag
    48541 gcgagaccga ggttgagcag atgcgagtgc aggcgttcca cctccagcag aatggcgcgg
    48601 atcatctgcg cacgttctgg cacctgaata cccatcgcgt tttctaccga cgtggtgtag
    48661 gcggtgctgt gggcaaagcc gcagatcccg cacacacggt cagagaggaa ggtcacttcg
    48721 ttgtaaccca tacgggtttc cgccagtttt tccatgccgc gatgaacgta gaacagacgg
    48781 tagtcggcgt cgataatgtt ttcgccatcg acgaacagac ggaagtggcc cggttcgtca
    48841 gaagtgacgt gcagcggacc aatcggcacg acgttgtttt tcttgtcgcc cagttcgttg
    48901 atgaactcgt aggtttcagc atcggtggtc ggtgccggac gctgacgata atccatgctg
    48961 tctttacgca gcggataaag ttcatccggc agcaccagac ggcgttcatc cggcagacca
    49021 accggaatca aaccgtacat atcgcgcact tcgcgctcgc cccacaccgc cgccggaacg
    49081 cgcggcgtca cggacggata ctccggtttg ttggcatcaa cttcgacgcg aaccgttacc
    49141 cagcacttag tccccttctc catcgacagc acgtaataaa cggcgtaatg accattcagt
    49201 ttgcgttcgt cgttaccaaa aagcaccgac aaccagcccc cctgcttgta gtagagaaac
    49261 tccaccactt ccggcagata gttcaccttt acggtgacag tcagctgatc tttggtctgc
    49321 caggcgtggt ccagcacgac gcccggaaat gcctcattca gcgcggcgag ataatgttga
    49381 cctaattttt cttcagacat gctcaaactc tctttaatca cgccgccagc aaggagacga
    49441 acgctaaaaa tgcaaagcca aacccagtcc aggtaatgcg cggagtaata tcaagacgca
    49501 gacgggccat gctgttttcg aacagcgcga taaccaggac gccgacgacc agtttgacga
    49561 tggcaatcac cagcgccagc agcagcccac cggcggtgaa ggtttccatc tgtccccacg
    49621 ggataaatac gccgacgaac atctgcagca ccaccagctg tttcaggctg ataccccatt
    49681 tcatgacgcc aaagccgctg ccgctgtatt cagagagcgg gccttcctgc agctcctgct
    49741 cggcttccgc caggtcgaac ggcagtttgc ccatttcgat aaaggtggcg aacgcacagg
    49801 cacaaagcgc cagtaccagc gggatgctct ggctcagcgg ccagtgataa acggtgtcgg
    49861 tgatgttgct gatgttggtg gaaccggcaa cctgtgcggc aacccacaga ccaagcagca
    49921 gcatcggttc gaccagcacg ccaagcatcg cttcacggct cgcgccgata gcggtaaacg
    49981 ggctaccggt atccagacca gaaatggcaa agaagaaacg cgcgatggca aagagataca
    50041 gtaaggtgat taaatcaccc agttgcggca gcggagaacc gacggtcacc accggcagcg
    50101 cagtagcgat agtcagcatg acgcccacca tcacatacgg cgtcaggcgg aacacccagc
    50161 cggaggcatc cgggccaacg ctctggcggc ccagcagttt gaaaatgtca cgatactcct
    50221 gcaacacacc cggcccacga cggttatgca gacgggcgcg cgccacgcgg gtaataccgg
    50281 agagcagcgg cgcaacggca aataacacca gcgcctgaat taacggatat aaaacactca
    50341 ttctcaggct cctcgtgaaa caataatcac caccagtacc gccagttcaa ccagcgccat
    50401 ccggcggaac agcaacgcac taccttcgca ctgccagccc ggcaccagcg acaccggatt
    50461 cagccatttg cgcagtttca gcactggcgc aaacgcctgt ttaaccggca tggcaaaacc
    50521 gtgagcggta atcaccattg atttttcatg gtcgtagccg cacacccagg ccgcaccgcg
    50581 ggaacgtgac ggcaggcggt cgcctttgca aatcgccata atgatgaatg gcagcagcgg
    50641 gcaggcaatc agcagcaatg tgatcattgg ttgagaaacg gtggtgttag caggctccag
    50701 aggcagaggt acagcagcag agagcatcgg cagcagccac ggcgcagcaa caccgccaat
    50761 tacgcagcaa atcgccagtg ccactacgct tacgctcatc aggagcggcg cacaggtggc
    50821 gttttcggct tctttggtac gcggcgcgcc gaggaacgtg acgccataga ctttcgccat
    50881 acacatcacc gccagcgcac cggtaattgc cagacccaca gcgagcagcg gccccagcag
    50941 acgggcaaca aacgcgccgc tattgctcag tttgaagaag gattgataga taacccattc
    51001 cccggcaaac ccgttcagtg gcggcagcgc agccattgcc atcaacccga ctaacatggc
    51061 gatggagata accggcattt tcttgccaat accaccgagt ttttcgatat cgcgatgacc
    51121 ggtacggaac cagacgctcc ccgccccgag gaacagtacg cttttgaaca ggctatggtt
    51181 aagcagatgg tacagaccac cgaccaggcc aagagcaatc agcgccggtt gttcgagcgc
    51241 gatacccgtt acgccagcgc ccagccccag caggataatg ccgatatttt ccagggtgtg
    51301 gtaagccagc aggcgctgga tgttgtgttc catcaacgca tacagaccac cgacgaacgc
    51361 ggtgatcatg ccgagcacca gcagcgcgat cccccaccac agcggtgcat taccgcccag
    51421 cagtgacagg gttaacatgc ccagcaggcc aattttcatg actaccgtag aaaacaacgc
    51481 ggcagctggc gcagaggcgt tagcatgtgc ctgcggcacc cagccgtgca gcggaataat
    51541 cccggccagc aggccaaagc caatcacacc gagcagccag atatcggaac cgagcggcag
    51601 ctgttgcata cgcatatcca gtaggcacag atccagcgtg ccataacgct gccacagcag
    51661 ccagcaagca atcaccagca gcagagtgcc aagacgcccc agcgcaaacc acagtttgcc
    51721 ctctttgctg ttgctggtga ggaacaccgc acacaaggcc atgacttcgg ccattaccac
    51781 gaacatgccg aggttgctgg cgatgacggc gcagacggcg gcagccatca acatattgat
    51841 ctgcaagccg ttgcacttca cctgcgcgtg gcgatgccag tcaatgttgt agaggctgac
    51901 aaacagaccg cacagaccga gcgtaatcag ccaaatcgcg ttaagcggag agatttgcac
    51961 atcgtagctt accagcgaca gcgcaccgct cacgccaacc gcgccagtca gtacagtgaa
    52021 gcccgcggct gccgtataca gactaccaac cgcgccgcca attccagcta tccagccact
    52081 taacgctttt tgaaaagaaa agagaaatgc cagaacagcg gcggcgacaa accatgccac
    52141 gccgctattg atcagggaaa ttgcgctcat ttagcctctc cactttgagc ctgctgaaac
    52201 aaggtgagat cgccaaagtc cgtgttaaag gtcagctcac gcttacgttt gctgacgcgg
    52261 gcgatatcgg tgttatccac cagatgcagg gctttagtcg ggcacatccg cacgcaggcc
    52321 ggaccttgtt catcaaaact gcaaaggtca catttgacgg cgatcgcgcg aatacccggc
    52381 acccagtcaa gcagcgtact gatacgcgcc ggagcaggcg gtgccggtgg cgctttcggg
    52441 gtattggcgt ttgccggaat atccagcgga cggctgccgg aaaattcaat tgcgccaaac
    52501 gggcaggcga tgccgcacag tttgcagctt acgcacagac tttcattcaa ctgcacggcc
    52561 ccatcaatgc gggtgatggc gttaaccggg caaaccaccg cacagggtgc atcttcacag
    52621 tggtgacaga gctgcggcgc agattctttt tcattcagca tcactctcag gcgcggcatt
    52681 gattgcaggc cgtgctggcg atgcgtctct gaacaggcgg cctcacaagt gtggcagccg
    52741 atacagagcg tggagtcaac aattacaaaa cgattcacca ggcattcctc aggtgattgt
    52801 catttttgac gaaaacatgc cgatgaaatg tcattttcga cactcatcga cacgcccatc
    52861 cccaaacagg cgtaacgcct gcaaaacggg caaagcctca gctcatgctg ccgggctttg
    52921 tccctttacc agttggctta aattcaccgg cacattgttt tcaatagcgg tgtataagct
    52981 gtcgtaaacg ccagcgattt tttcgagata gtgttccaga acctgttcgc gatcgcggat
    53041 gttgaccttg ccatcaatca tgccgtcaat gctcagttgc gcctgcgcaa tccgctgttt
    53101 gtcttcgcca tcttttgttt cgacgtaata ctcgatggtg tggctgttaa agccatccgc
    53161 cagggtgacg taaatacgaa aatgttcaaa aaggacgaaa cagagttctt tgtccggcgc
    53221 gcagctcatg gcgtgatgca ggcgcgcttt cgataacgtg atcccctgaa ccagcgaatt
    53281 gcagtagatg tgccactggt cctgtaggcg acgatgccgc tgtgcgatgt aatcggcttt
    53341 ctcgcttatt tcccaaatag tcattgtcag gttacccgtt taacagagat gccagcttta
    53401 agcattttct atgccaactt ttaatttatt gttattaaat agatttttaa gctaaagatg
    53461 aatttcgtcg ccgtgtcgac gtgtcatttc gacatcatcg acattattca ccgcagggat
    53521 aatcaacact ggcacaatta ttgcttgtag ctggcaatag ttaatgggag gcgatatgca
    53581 cgaaataacc ctctgccaac gggcactgga attgatcgaa cagcaggccg caaaacacgg
    53641 cgcaaaacgc gtaactgggg tctggctcaa aattggcgca ttttcttgtg tcgaaaccag
    53701 ctctcttgcc ttttgttttg atctggtttg ccgcggcagc gtggcggaag gttgtaaact
    53761 gcacctcgaa gaacaagagg ccgaatgctg gtgtgaaaca tgccaacagt atgtgacgct
    53821 actgacccag cgcgtccgcc gctgtccaca gtgtcatggt gacatgctac agattgtggc
    53881 agacgacggt ttacagattc ggcggataga aatagaccag gagtgagcga tgtgtacaac
    53941 atgcggttgc ggtgaaggca acctgtatat cgagggtgat gaacataacc ctcattccgc
    54001 gtttcgtagc gcgccatttg ccccggcggc acgcccgaag atgaaaatca ccggcattaa
    54061 agcgcctgaa tttaccccca gccagactga agaaggcgac ctgcattacg gtcatggcga
    54121 agcgggcact cacgcgccgg gcatgagcca gcgtcggatg ctggaagtcg aaattgacgt
    54181 gctggacaaa aataaccgtc tggctgaacg caaccgcgcg cgctttgctg cccgcaagca
    54241 actggtgctc aacctggttt ccagccctgg ttccggtaaa accaccctgc tgacggaaac
    54301 cctaatgcgc ctgaaagaca gcgttccgtg cgcggttatt gaaggtgacc agcaaaccgt
    54361 gaacgatgcc gcacgcattc gcgctaccgg cacaccagcg attcaggtga acaccggtaa
    54421 aggctgccat cttgacgcac agatgattgc cgacgccgca ccgcgtctgc cactggacga
    54481 taacggtatt ctgtttatcg aaaacgttgg caacctcgta tgcccggcca gcttcgatct
    54541 cggtgaaaaa cacaaagtgg cggtgctttc cgttaccgaa ggtgaagaca aaccgctgaa
    54601 atatccgcat atgtttgccg ccgcctcgct gatgctgctc aacaaagttg acctgttgcc
    54661 gtatctcaac tttgacgttg agaagtgcat cgcctgcgcc cgcgaagtca atccagaaat
    54721 tgaaatcatc cttatttccg ccaccagcgg cgaagggatg gaccagtggc tgaactggct
    54781 ggagacacag cgatgtgcat aggcgttccc ggccagatcc gcaccattga cggcaaccag
    54841 gcaaaagtcg acgtctgcgg cattcagcgc gatgtcgatt taacgttagt cggcagctgc
    54901 gatgaaaacg gtcagccgcg cgtgggccag tgggtactgg tacacgttgg ctttgccatg
    54961 agcgtaatta atgaagccga agcacgcgac actctcgacg ccttacaaaa catgtttgac
    55021 gttgagccgg atgtcggcgc gctgttgtat ggcgaggaaa aataatgcgt tttgttgatg
    55081 aatatcgcgc gccggaacag gtgatgcagt taattgagca tctgcgcgaa cgtgcttcac
    55141 atctctctta caccgccgaa cgccctctgc ggattatgga agtgtgcggt ggtcataccc
    55201 acgccatttt taaattcggc ctcgaccagt tactgcctga aaacgttgag tttatccacg
    55261 gtccgggttg cccggtgtgc gtactgccga tgggtagaat cgacacctgc gtggagattg
    55321 ccagccatcc ggaagtcatc ttctgtacct ttggcgacgc catgcgcgtg ccggggaaac
    55381 agggatcgct gttgcaggca aaagcacgcg gtgccgatgt gcgcatcgtt tactcgccga
    55441 tggatgcgtt gaaactggcg caggagaatc caacccgtaa agtcgtattc ttcggcttag
    55501 gttttgaaac caccatgccg accaccgcca tcaccctgca acaggcgaaa gcccgcgatg
    55561 tgcagaattt ttacttcttc tgccagcata ttacgcttat cccgacgctg cgcagtttgc
    55621 tggaacagcc ggataacggt atcgacgcgt tcctcgcgcc gggccacgtt agtatggtta
    55681 tcggcactga tgcctataat tttatcgcca gcgattttca tcgtccgctg gtggtcgctg
    55741 gtttcgaacc ccttgatcta ctgcaaggcg tggtcatgct ggtggagcag aaaatagcgg
    55801 cccacagcaa ggtagagaat cagtatcgtc gggtggtacc ggatgccggt aacctgctgg
    55861 cgcaacaggc aattgctgat gtgttctgtg tcaacggcga cagcgaatgg cgcggcttag
    55921 gcgtgattga atcttctggc gtgcacctga cgccggatta tcaacgattc gatgccgaag
    55981 cacatttccg cccagcaccg cagcaggtct gcgatgaccc gcgcgcgcgt tgtggtgagg
    56041 tattaacggg caaatgtaag ccgcatcaat gcccgctgtt tggtaacacc tgtaatcctc
    56101 aaaccgcatt tggtgcactg atggtctcct ccgaaggagc gtgcgccgcg tggtatcagt
    56161 atcgtcagca ggagaatgaa gcgtgaataa tatccaactc gcccacggca gcggcggcca
    56221 ggcgatgcag caattaatca acagcctgtt tatggaagcc tttgccaacc cgtggctggc
    56281 agagcaggaa gaccaggcac gtcttgatct ggcgcagctg gtagcagaag gcgaccgtct
    56341 ggcgttctcc accgacagtt acgtcattga cccgctgttc tttcctggcg gtaatatcgg
    56401 caagctggcg atttgcggca cagccaatga cgttgcggtc agtggcgcta ttccgcgcta
    56461 tctctcctgt ggctttatcc tcgaagaagg attgccgatg gagacactga aagccgtagt
    56521 gaccagcatg gcagaaaccg cgcgcgcggc aggcattgcc atcgttactg gcgatactaa
    56581 agtggtgcag cgcggcgcgg cagataaact gtttatcaac accgctggca tgggcgcaat
    56641 tccggcgaat attcactggg gcgcacagac gctaaccgca ggcgatgtat tactggttag
    56701 cggcacgctc ggggaccacg gggcgactat ccttaacctg cgtgagcagc tggggctgga
    56761 tggcgagctg gtcagcgact gcgcggtact gacgccgctt attcagacgc tgcgtgacat
    56821 tcccggcgtg aaagcgctgc gtgatgccac ccgtggtggt gtgaacgcgg tagttcatga
    56881 gttcgcggca gcctgcggtt gtggtattga aatttcagaa gtggcactgc cggttaaacc
    56941 tgccgtgcgc ggcgtttgcg aactgctggg actggacgcc cttaactttg ccaacgaagg
    57001 caaactggtg atcgccgttg aacgcaacgc ggcagagcaa gtgctggcag cgttacattc
    57061 ccatccactg gggaaagacg cggcgctaat tggtgaagtg gtggaacgta aaggtgttcg
    57121 tcttgccggt ctgtatggtg tgaaacgaac cctcgattta ccacacgccg aacctcttcc
    57181 gcgtatatgc taataaaatt ctaaatctcc tatagttagt caatgacctt ttgcaccgct
    57241 ttgcggtgct ttcctggaag aacaaaatgt catatacacc gatgagtgat ctcggacagc
    57301 aagggttgtt cgacatcact cggacactat tgcagcagcc cgatctggcc tcgctgtgtg
    57361 aggctctttc gcaactggta aagcgttctg cgctcgccga caacgcggct attgtgttgt
    57421 ggcaagcgca gactcaacgt gcgtcttatt acgcatcgcg tgaaaaagac accccattaa
    57481 atatgaagac gaaactgttc tggcacacgg tccggtacgc cgcattttgt cgcgccctga
    57541 tacgctgcat tgcagttacg aagaattttg tgaaacctgg ccgcagctgg tcgcaggtgg
    57601 gctatacccc aaatttggtc actattgcct gatgccactg gcggcggaag ggcatatttt
    57661 tggtggctgt gaatttattc gttatgacga tcgtccctgg agcgaaaaag agttcaatcg
    57721 tctgcaaaca tttacgcaga tcgtttctgt cgtcaccgaa caaatccaga gccgcgtcgt
    57781 caacaatgtc gactatgagt tgttatgccg ggagcgcgat aacttccgca tcctggttgc
    57841 cattaccaac gcggtgcttt cccgcctgga tatggacgaa ctggtcagcg aagtcgccaa
    57901 agaaatccat tactatttcg acattgacga tatcagtatc gtcttacgca gccaccgtaa
    57961 aaacaaactc aacatctact ccactcacta tcttgataaa caacatcccg cccacgaaca
    58021 gagcgaagtc gatgaagccg gaaccctcac cgaacgcgtg ttcaaaagta aagagatgct
    58081 gctgatcaat ctccacgagc gggacgattt agccccctat gaacgcatgt tgttcgacac
    58141 ctagggcaac cagattcaaa ccttgtgcct gttaccgctg atgtctggcg acaccatgct
    58201 gggcgtgctg aaactggcgc aatgcgaaga gaaagtgttt accactacca atctgaattt
    58261 gctgcgccag attgccgaac gtgtggcaat cgctgtcgat aacgccctcg cctatcagga
    58321 aatccatcgt ctgaaagaac ggctggttga tgaaaacctc gccctgaccg agcagctcaa
    58381 caatgttgat agtgaatttg gcgagattat tggccgcagc gaagccatgt acagcgtgct
    58441 taaacaagtt gaaatggtgg cgcaaagtga cagtaccgtg ctgatcctcg gtgaaactgg
    58501 cacgggtaaa gagctgattg cccgcgcgat ccataatctc agtgggcgta ataatcgccg
    58561 catggtcaaa atgaactgcg cggcgatgcc tgccggattg ctggaaagcg atctgtttgg
    58621 tcatgagcgt ggggctttta ccggtgccag cgcccagcgc attggtcgtt ttgaactggc
    58681 ggataaaagc tctctgttcc tcgacgaagt gggcgatatg ccactggagt tacagccgaa
    58741 gttgctgcgt gtattgcagg aacaggagtt tgaacgtctc ggcagcaaca aaatcattca
    58801 gacggacgta cgtttaattg ccgcgactaa ccgcgatctg aaaaaaatgg tcgccgaccg
    58861 agagttccat agcgatctct attaccgcct gaacgtattc ccgattcacc tgccgccact
    58921 acgcgagcgt ccggaagata ttccgctgct ggcgaaagcc tttaccttca aaattgcccg
    58981 tcgtctgggg cgcaatatcg acagcattcc tgccgagacg ctgcgcacct tgagcaacat
    59041 ggagtggccg ggtaacgtac gcgaactgga aaacgtcatt gagcgcgcgg tattgctaac
    59101 acgcggtaac gtgctgcagc tgtcattgcc agatattgtt ttaccggaac ctgaaacgcc
    59161 gcctgccgca acggttgtcg cccaggaggg cgaagatgaa tatcagttga ttgtgcgcgt
    59221 gctgaaagaa accaacggcg tggttgccgg gcctaaaggc gctgcgcaac gtctggggct
    59281 gaaacgcacg accctgctgt cacggatgaa gcggctggga attgataaat cggcattgat
    59341 ttaactgcaa attgccggac agatctgcct gtccggcata ctattcacga ggttttttcg
    59401 gacgatattt ttccggcagt tctggtaccg gacgcttgtc atcgatgaga tgacgcacgg
    59461 ttaagatcgg atgacgccac agcattctcg gcccggccca acgcataatc tgtttcatct
    59521 cttcacgctt tgcaggctgg taacagtgca ccggacactg cttacaggct ggtttctctt
    59581 cgccgaacac acatttatcc agccgctttt gcgcgtaaac aaacaacgcc tcgtaatgct
    59641 ccggctccgc tgacgcctgc gggcatttcg cttgataaag atcgatcatt tttttaatcg
    59701 tcagtttttc acgagagata cgcttgccgg acatgctgcc tccatactct taaggtgtat
    59761 ttatattaca acttaatttg aaaggggact atggctctga aggaggaggt ttattcaacc
    59821 gacgcaattt tgcgtacttg cttcataagc atcacgcaaa agctgcaaaa cagcatcttt
    59881 cccggaacca gcatcaagaa ctcgccgttc gcttcttccc ctgaaatgat taactccggt
    59941 atcatgtgcg ccttatgtga ttacaacgaa aataaaaacc atcacacccc atttaatatc
    60001 agggaaccgg acataacccc atgagtgcaa tagaaaattt cgacgcccat acgcccatga
    60061 tgcagcagta tctcaggctg aaagcccagc atcccgagat cctgctgttt taccggatgg
    60121 gtgattttta tgaactgttt tatgacgacg caaaacgcgc gtcgcaactg ctggatattt
    60181 cactgaccaa acgcggtgct tcggcgggag agccgatccc gatggcgggg attccctacc
    60241 atgcggtgga aaactatctc gccaaactgg tgaatcaggg agagtccgtt gccatctgcg
    60301 aacaaattgg cgatccggcg accagcaaag gtccggttga gcgcaaagtt gtgcgtatcg
    60361 ttacgccagg caccatcagc gatgaagccc tgttacagga gcgtcaggac aacctgctgg
    60421 cggctatctg gcaggacagc aaaggtttcg gctacgcgac gctggatatc agctccgggc
    60481 gttttcgcct gagcgaaccg gccgaccgcg aaacgatggc ggcagagctg caacgcacta
    60541 atccggcgga actgctgtat gcagaagatt tcgccgagat gtcgctgatt gaaggccgtc
    60601 gcggcctgcg ccgtcgcccg ctgtgggagt ttgaaatcga caccgcgcgc cagcagttga
    60661 atctgcaatt tggcacccgc gatctggtcg gttttggcgt cgagaacgcg ccgcgcggac
    60721 tttgtgctgc cggttgtctg ttgcagtatg cgaaagatac ccaacgcacg accctgccgc
    60781 atattcgttc tatcaccatg gaacgtgagc aggacagcat cattatggat gccgcgacac
    60841 gtcgtaacct ggaaattact cagaacctgg cgggcggtgc ggaaaatacg ctggcttctg
    60901 tgctcgactg caccgtcacg ccgatgggta gtcgtatgct gaaacgctgg ctgcatatgc
    60961 cagtgcgcga tacccgcgtg ttgcttgagc gccagcaaac tattggcgca ttgcaggatt
    61021 tcaccgccga gttgcagccg gtactgcgtc aggtcggcga cctggaacgt attctggcac
    61081 gtctggcttt acgaactgct cgcccacgcg atctggcccg tatgcgccac gctttccagc
    61141 aactgccgga gctgcgtgcg cagttagaaa ctgtcgatag tgcaccggta caggcgctac
    61201 gtgagaagat gggcgagttt gccgagctgc gcgatctgct ggagcgagca atcatcgaca
    61261 caccgccggt gctggtacgc gacggtggtg ttatcgcatc gggctataac gaagagctgg
    61321 atgagtggcg cgcgctggct gacggcgcga ccgattatct ggagcgtctg gaagtccgcg
    61381 agcgtgaacg taccggcctg gacacgctga aagttggctt taatgcggtg cacggctact
    61441 acattcaaat cagccgtggg caaagccatc tggcacccat caactacatg cgtcgccaga
    61501 cgctgaaaaa cgccgagcgc tacatcattc cagagctaaa agagtacgaa gataaagttc
    61561 tcacctcaaa aggcaaagca ctggcactgg aaaaacagct ttatgaagag ttgttcgacc
    61621 tgctgttgcc gcatctggaa gcgttgcaac agagcgcgag cgcgctggcg gaactcgacg
    61681 tgctggtgaa cctggcggaa cgggcctata ccctgaacta cacctgcccg accttcattg
    61741 ataaaccggg cattcgcatt accgaaggtc gccatccggt agttgaacaa gtactgaatg
    61801 agccatttat cgctaacccg ctgaatctgt cgccgcagcg ccgtatgttg atcatcaccg
    61861 gtccgaacat gggcggtaaa agtacctata tgcgccagac cgcactgatt gcgctgatgg
    61921 cctacatcgg cagctatgta ccggcacaaa aagtcgagat tggacctatc gatcgcatct
    61981 ttacccgcgt aggcgcggca gatgacctgg cttccgggcg ctcaaccttt atggtggaga
    62041 tgactgaaac cgccaatatt ttacataacg ccaccgaata cagtctggtg ttaatggatg
    62101 agatcgggcg tggaacgtcc acctacgatg gtctgtcgct ggcgtgggcg tgcgcagaaa
    62161 atctggcgaa taagattaag gcattgacgc tgtttgccac ccactatttc gagctgaccc
    62221 agctaccgga gaaaatggaa ggcgtcgcca acgtacatct cgatgcgctg gagcacggcg
    62281 acactatcgc ctttatgcac agcgtgcagg acggcgcggc gagcaaaagc tacggtctgg
    62341 cggttgctgc tctggcgggc gtaccgaaag aggttattaa gcgcgcacgg caaaaactgc
    62401 gtgagctgga aagcatttcg ccgaacgccg ccgcgacgca agtggatggt acgcagatgt
    62461 ctttgctgtc agtgccagaa gaaacttcgc ctgcggtcga agcactggaa aatctcgacc
    62521 cggattcact gactccgcgt caggcgctgg agtggattta tcgcttgaag agtctggtgt
    62581 aataacaatt cccgatagtc ttttgctatc gggaatatta acgacaactg acgaatcaaa
    62641 taaaaatacc ctgtataata ggaaagctta ttttacaggg taaaaccatg ccatctacac
    62701 gctatcaaaa aatcaatgcc catcactatc gccatatatg ggtcgttggt gatattcatg
    62761 gtgaatatca gttattacaa tcccgttaac atcaactctc tttttaccct gaaaccgact
    62821 tacttatttc cacaggcgat aatattgatc gtgggccgaa gagtcttaac gtgctgcgcc
    62881 tgctaaacca accctggttt acgtcggtta aaggcaacca cgaagcgatg gcgttggatg
    62941 cattcgaaac tggcgatgga aatatgtggc ttgccagcgg tggtgactgg tttttcgatt
    63001 taaatgattc agagcaacaa gaggcaatag atctgttgct gaaattccat caccttccac
    63061 atattattga aatcattaat gacaacataa aatatgccat c
//
LOCUS       DAZPDU010000005        49871 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZPDU010000005 DAZPDU010000000
VERSION     DAZPDU010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49871
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.005"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(443..1789)
                     /gene="yagA"
                     /locus_tag="ACHURS_000267"
     CDS             complement(443..1789)
                     /gene="yagA"
                     /locus_tag="ACHURS_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001132032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YagA"
                     /protein_id="HFR1236095.1"
                     /translation="MPMILVKKNPRGKVIRELSSEEETAVKTVCGLKKPATMALHNLA
                     NDILREMREYDAWLQCDCIPGDSPAMNFAALKNNTGTLYLSSFNHEHAPECPMYRQLS
                     GNEEETSSGASRHPVSTRINYRNFLPPDDSNSVIKLQARSAYHNGESSSVRKKRPRLA
                     RLLLSLIDDAGLNKLDSLANPRIRTNYRECLDAIRQVTLQQEYIRGRSLSEIIHFRPG
                     MSERSQERLMETLENSERHWPARRKHMFFQIFMAQHICRDAVEIHWANGNIQVIRPVR
                     GISINGEAQGGIRPPYWVILAFCRSADGRIICSEGYAHALYQLTCPVPVDSKLERNTL
                     TALLNVASWLKRKPGTPELSLERPLFDTEVYVNGEKKYVLPDFIVTARAPDGKTARVV
                     IETMGYEDSDYCARKSRQHTGMKQIGVLHTDPPKWLDNDHPPFEKHMYGVFMHLRY"
     gene            2143..2745
                     /locus_tag="ACHURS_000268"
     CDS             2143..2745
                     /locus_tag="ACHURS_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFR1236096.1"
                     /translation="MGKNQEERKTPVIVVKKRRTFSPPSLSEKTDIIAPVFTEQTAES
                     APAGINSSAVETHIPEAPARKKKKKRHRFPRPSHWTREYTHECVEKIKALFPHLRAEG
                     GGFIPLKIGINNDISAFLAEHPETELTMDEWLCAVSCITSRRVYLQRTAVAGVPRYGL
                     DGHPKGQVSDSEAQSAGRRLATLEQKWLRMQAQQENISGQ"
     gene            2983..3249
                     /locus_tag="ACHURS_000269"
     CDS             2983..3249
                     /locus_tag="ACHURS_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1236097.1"
                     /translation="MITGNGINTVTVNGKVKHITELDDITLCLEWTKLREENNRLYEI
                     NNQANRGWRGFILRLIGVNLPDKRTEFTQRLLLTRKISGSVMKK"
     gene            complement(3440..3721)
                     /locus_tag="ACHURS_000270"
     CDS             complement(3440..3721)
                     /locus_tag="ACHURS_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1236098.1"
                     /translation="MNYDEITKITAERISDYMTEAVNTDSIAVAEMFHNAAWGVRTLW
                     FELVTKIDIDIHKKNRYASYDLRRKIEMQHEEFQKMTEREQVPLLKSPE"
     gene            complement(3827..4102)
                     /locus_tag="ACHURS_000271"
     CDS             complement(3827..4102)
                     /locus_tag="ACHURS_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR1236099.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIYVLRLWHTRENR"
     gene            complement(4102..4386)
                     /locus_tag="ACHURS_000272"
     CDS             complement(4102..4386)
                     /locus_tag="ACHURS_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HFR1236100.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAWADSLSTDNPLPVPR"
     gene            complement(5294..6325)
                     /gene="repA"
                     /locus_tag="ACHURS_000273"
     CDS             complement(5294..6325)
                     /gene="repA"
                     /locus_tag="ACHURS_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiator RepA"
                     /protein_id="HFR1236101.1"
                     /translation="MAGLKNTSYNAVHWSQLAPEEQIRFWEDYEAGRATTFLVEPERK
                     RTKRRRGEHSTKPKCENPSWYRPERYKALKGQLGHAYNRLVKKDPVTGEQSLRMRMSR
                     HPFYVQKRTFVGRKYAFRPEKQRLLDAIWPVLVSFSDAGTHTVGMSVTRLAEEISPKD
                     SEGHVIPELEVTVSRLSRLLAEQVRFGVLGVSEETMWDREHRQRLPRYVWITPAGWQM
                     LGVDMVKLHEQQQKRLRESEIRQQLIREGVLREDEDISVHAARKRWYLQRSQDALKKR
                     REKAAASKRANRLKKLPVDQQIYEMAEYLRKRLPPDEAYFCSDDHLKRLAIRELRQLE
                     LTLAAPPPH"
     gene            7026..7667
                     /locus_tag="ACHURS_000274"
     CDS             7026..7667
                     /locus_tag="ACHURS_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HFR1236102.1"
                     /translation="MCLSELQARLNVGQPLFPTHALCIRRSQQHRSPPAGIDFRGAFR
                     ILNISELHQRNWYLAQYIPAGKNREHLFSWLSEQHVLPWTPLILKKVRRTDKVCGYRR
                     HIHAVFPGYFFLKADPESHSFTHLRRHSAFLDFVKMAGEIKTVREDIVQSLMKVYPDP
                     ALNPAAREELDAASTLWLTKARYQYLLRLDAQPLPESRIALLLHLVSDDGALT"
     gene            7808..8470
                     /locus_tag="ACHURS_000275"
     CDS             7808..8470
                     /locus_tag="ACHURS_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="traC"
                     /protein_id="HFR1236103.1"
                     /translation="MTILSQENPVILLLENMLEALSTAPDNINNERRRRRYLLNWLDT
                     ARQMREFRGMAEEFTTLRKLLATNSDIPVANTLKSLLESGNNAALYDLYRFRSALATC
                     RSLGWQIGTCAWNDQLLSETLSRAQTGKRHILQLNSTGDTFSTTGRMIRPVAFSLIHP
                     ATINTSEVEEIFRDEGFMLAHGRECSLNGSGRNMLSRILHVGHSGLAEEEWGMDNSLL
                     LH"
     gene            8737..9357
                     /locus_tag="ACHURS_000276"
     CDS             8737..9357
                     /locus_tag="ACHURS_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFR1236104.1"
                     /translation="MTLTLLTAYNAPYLAALTFVLLTGIAELIALLCGHSLSSAMDTP
                     DLPEGLTGEALDWLNIGRIPLLIVLCMLAGFFGISGILLQGLIIHLLQAPAPNILLAP
                     LCLLLTCPLVHRTGRLIAPWLPRDETTALTEEAFIGCMATITGYHAVAGSPCEGRFTD
                     RFGQVHYLLLEPEAGKEFRRGDKVIIVCRLSGSRWLGELNPWPDVL"
     gene            9384..11078
                     /locus_tag="ACHURS_000277"
     CDS             9384..11078
                     /locus_tag="ACHURS_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFR1236105.1"
                     /translation="MFNLPGWLFTVIALVLTLFVIGLIFARLYRRASAEQAFVRTGLG
                     GQKVVMSGGAIVMPIFHETIPVNMNTLKLEVSRAAAESLITRDRMRVDVAVAFFLRVK
                     PSAEGISTAAQTLGQRTLTPEDLRSLVEDKFVDALRATAARMSMQDLQDARENFVQGV
                     QNTVAEDLSKNGLELESVSLTSFNQTARVHFNPDNAFDAEGLTLLTQETERRRRERNE
                     VEQDVEVAIREKNRDALSRRLEIEQQEAFMTLEQQQRVKTRTAEQSASIAAIEAERRR
                     EAESARILAERKIEEAEIERQQIVRTRQVEAEREVAIREIEQQQATEIASQARAIAVA
                     AKSEEQSQAEARASKALAEAVQAQQDVETTRQTAEADRTKQVALIAAAQEAETKAVEL
                     TVRAQAEKEAAELQAAAIIELAEATRQKGLAEAEAQRALNDAINVLSDEQTSLRFRLA
                     LLDALPAVIEKSTEPMKSIDGIKIIQVDGLNGGVTRGKTATTDISGGNLAEQAVTAAL
                     AYRTQAPLIDSLLKEVGLSGEGLTALTRPLVMPAPEKVPDVSEPVTAPDNSEPQQV"
     gene            11159..11401
                     /locus_tag="ACHURS_000278"
     CDS             11159..11401
                     /locus_tag="ACHURS_000278"
                     /inference="COORDINATES: protein motif:HMM:NF022090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="HFR1236106.1"
                     /translation="MSAEKFRVVIYTNTCEKKVSYIPATEDINIAIMPFQTADNGLII
                     VCQNSRIIRRKERIPGSSCEWKDVTLAPKSRNRVQL"
     gene            11517..11852
                     /locus_tag="ACHURS_000279"
     CDS             11517..11852
                     /locus_tag="ACHURS_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1236107.1"
                     /translation="MRTITTREQLLINGRVHERIATHIVTGAHGYETLCTNGYNVRYN
                     KAQQLVESCEKIAEGKLPVTCPVCFTIWQDVHRFTHVDFDTQSGKSDFIDTCHSEIIT
                     GHVSITGKK"
     gene            12079..13149
                     /locus_tag="ACHURS_000280"
     CDS             12079..13149
                     /locus_tag="ACHURS_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpQ domain-containing protein"
                     /protein_id="HFR1236108.1"
                     /translation="MKKKSLTLLLPSLLSGCTAFNATPVPDTTPPALVFVDGQIRESA
                     AIIAHTQKRLLPPPVTRPVTPPRPTTTATPTTAVSSQSTALPPRPLLQATQLPALVMT
                     GKPGQSSVVYLREAHQRSVSDWIRQLLPPGWQFRQQNAAIPALNKRLPVWSVNDQWHR
                     ALNALLAGQQLYGHMDWNRKILTVTTSATPPVDVTAPQGSQKAADTPRNPFRGSTATP
                     AGPTPVTPAVSVSRAQTTTGTSGTPESRTTTPAPVATKPVVPPQQKVIPPRQWKMEKG
                     TTLKDGLARWTSSEKCPHGTWTLRWETPVNYSIDAPLVFTGSFRQALEQLFALYTPAE
                     KPLYARTSTAQCLLRVTDRPQE"
     gene            13153..13590
                     /gene="pilM"
                     /locus_tag="ACHURS_000281"
     CDS             13153..13590
                     /gene="pilM"
                     /locus_tag="ACHURS_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HFR1236109.1"
                     /translation="MGWVTGFIMVIMAIGNFWFHSHLSRTVHHQQTAEITQQAADFIR
                     YMNAINDYLYQHPERRAAGGQLTSAQLGLPATKNVSHLISQQRVFVWAKEKPGLMGAL
                     LEQSGDSALLARVENGRLLDTHGRRISITLPAVIPDQVIIWMN"
     gene            13622..15241
                     /locus_tag="ACHURS_000282"
     CDS             13622..15241
                     /locus_tag="ACHURS_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HFR1236110.1"
                     /translation="MNKKLLAIMIAATLLQGCTSFRQAENTRKDAVAEQQRAREMIAD
                     LRNRTPVVKESPRQWINPRPVTDKNPGNAVPGCPIIINTRQSITLQQVAQRISENCHI
                     PVRITPDVQAYLSVTTSGSTQQIQGTVPPPDAGGMVPLAAIGSSDTTLPRLQGNTASL
                     PELKASGLTSLLNAVASRLGISWRYDGSHITFYYLDTRTFPVTYMDSQVAYNATVVSG
                     TMSSNGSSGGTASDAISGDASNTQTTTVDMKSALYQDVKNAVSSMLTPGIGRMFMSTG
                     FITVTDTPQVLETVRTFIEKRNEEMKRQVVLNVEILSIRKTRNEQAGIDWNAVFSDRT
                     LGLSLGSTFTSAASDTVTGGVSIVNGKLTGSKAFLKALSSQGDVSVVTRNSAVTKNLT
                     PVPMQIANQQSYIESVTTDTTANVGSSTSLNAATITTGFNMTLLPFILPDSQTLQLLY
                     SMSLSDKPVIENYESGGSKAQLPNVDLKTINQTVDLKSGQTVIISGFQQSGRRSGKQG
                     VGTPGFFGLGGGINSENDDTILVVLITPNII"
     gene            15262..16557
                     /gene="pilO2"
                     /locus_tag="ACHURS_000283"
     CDS             15262..16557
                     /gene="pilO2"
                     /locus_tag="ACHURS_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HFR1236111.1"
                     /translation="MTDISPTERQVFPLTAGRNVFLAGMDWKTLPASHKNPRTFARSL
                     GAVRYISCEYLSTEDTDRHIMVAAVSQNTLPKGSRRYFSLAMLILPLLESGGYAIVEL
                     SQANDTELYGFVSAVDGILVSDLVGTREEIREAREIFLTINSAPEHGWTCYEPPSFNG
                     PDGRGPLPLETLTGAGKYPAEARLHPVSRGPQLIPVLLILTLLGTAWYGWQYYERLKA
                     EKAALAEAAQKAAEERITPPWLSMPETGDFIRGCSDVWSRLPVSVAGWRYSLAECAAT
                     GNSGNLRASYTNIAGTTVTAFSQRVREIFSIRPFMVMPDGNSGGFALPVTFSMPETPV
                     AVEALPENTLLQERLTTLARSMQLKMDWQEVSNSFTDEDGNTIQPPWKEYDLQILTTL
                     PAHQVAEHFSEPSVRFISVTRQLEEGRFRYQFTGKYYVQ"
     gene            16547..17005
                     /gene="pilP"
                     /locus_tag="ACHURS_000284"
     CDS             16547..17005
                     /gene="pilP"
                     /locus_tag="ACHURS_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HFR1236112.1"
                     /translation="MSSKTKALLLSVLILPTTVAAGTISNMQQNNRQLQVEQIQEETA
                     LYEAMLAREKVRHELRTLEQGEPAEPVHSQESSSAVTTDTPAPITGIPRLTEISGVQP
                     QITARIQLPGGRTADVKEGQHIPGTTFRVEKITTDSVVLEQNGIRTTLQP"
     gene            17108..18616
                     /locus_tag="ACHURS_000285"
     CDS             17108..18616
                     /locus_tag="ACHURS_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HFR1236113.1"
                     /translation="MLAPADQPPDDILDYVVVDTDKTGQIRVRVVEGKKHLRAVQDYL
                     TRLCSRYPGKVSPFEFTTLDVIARLRQETVISGVENGINPVQQKMLGYISHAATLSAS
                     DLHITPGRDSTDFTYLEARVHGELELLDIVRKDEGLELLGATYSGMTDVIKGTQFDPG
                     VPQDARLAERFLKLAGLFGARYSHYPCVGGLYAVLRLIKDDSQHIPTFSMLGYHPEQE
                     RAVRRMLQRPEGIVILSGPTGSGKSTTLRTASAAYLEQYGFNDTGGILLPRRRLFTIE
                     SPPEGRIPGAIQTAVMDTAQGWVDSVKSALRLDPDAILNGEIRDHASAITAIKAAMTG
                     HLMLTTLHANDPINILERLEMEGVQARMIADPQLFIGLLSQRLVQVICPHCRLPWHEV
                     ESSRTDEERRLVENFCQPDAVYLRNHNGCPHCWRGVNGRTVIAEVISPDAKFFQIYRE
                     KGRIEAKTYWHRELGGMTRNQHLLGKINSGQVDPLAAHYISPVDEDSYTLLH"
     gene            18618..19712
                     /locus_tag="ACHURS_000286"
     CDS             18618..19712
                     /locus_tag="ACHURS_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HFR1236114.1"
                     /translation="MKILERLNNTELELSGLNRWLGKKTFRRTTFYESLAGMIRDGTP
                     VMRALEFICDVETDFGKKKGQSGLYFLATDCIASIRSSGQLSPALKDWVPKDEIALIR
                     NGEERGDIAEAMFQVVKTAKGRQEMISSLVSVCLYPLILLTLCVVNMYNVHTRVIPMI
                     SAFAPEERWSVQMVILAKSSELVIGYGLWLLAGLLLLAILIRMSFSLYKGPRRRYLDR
                     IPPWSLYQTFHGVNYLFNISSMLQINIPLSHALTRIEKHAEHNRWLKSRISAIRKNVL
                     SGQSLALAMKNSGYDFPSKSCVNNLLLNSEREDSVASMALYADRWLEEAKKSVRKSGI
                     IITAISGLLVFLFIINMVSAIYGISDMLKQ"
     gene            19774..20310
                     /locus_tag="ACHURS_000287"
     CDS             19774..20310
                     /locus_tag="ACHURS_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus major pilin"
                     /protein_id="HFR1236115.1"
                     /translation="MSVITCSYSEHNIRHLQNDDKGMTLLEVLGVLVVAAIVIGAVMG
                     LMSDTLSSSDNQKELKNLQTIATKMKAQKFQGQYTGTDYVKILTESGGLPADMIAGGN
                     KAKNAWGGAVTIKVSSDKYSYVIESSNVPKKNCIDLVTSLRSSSMFTKINGNVTNKVD
                     PSTVCNADKTTIKLETNS"
     gene            20355..20840
                     /locus_tag="ACHURS_000288"
     CDS             20355..20840
                     /locus_tag="ACHURS_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFR1236116.1"
                     /translation="MNRLFILTVIFSLLCSLPSAQASPQDKQYDRCFITAGKRYSIDP
                     GLLKIIARRESSLNPRAINHNRNDRGKIISTDYGIMQINSTHIPGLKKMGIIRSEKDL
                     LNNVCLNIQTGAWILARHFQRCGVNWECLGSYNAGFSKSNTHRRMKYARQIYSAYMQG
                     R"
     gene            20856..21482
                     /locus_tag="ACHURS_000289"
     CDS             20856..21482
                     /locus_tag="ACHURS_000289"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFR1236117.1"
                     /translation="MLQMIPALLVAITVVIQCLAVQMSRYRLSRMNARPPHHGLALMS
                     GMLAAAAIILRLYQNDSAVGVLFMTIMTGCMTLMTLTDAASCRLPRPFTVMYLISGSA
                     FRLWQGDWLTGLCSAGFWFLLLLLFRQYTSYRRKTETIGLGDVFLIAGIAIWSPPGDI
                     PWIVATAAGGALLYLRCNRQQRITRELPFGPFLCASQYAFTFYPGGLW"
     gene            21500..22861
                     /gene="pilV"
                     /locus_tag="ACHURS_000290"
     CDS             21500..22861
                     /gene="pilV"
                     /locus_tag="ACHURS_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HFR1236118.1"
                     /translation="MQKDNDKGFALLEILGGLVVISLLMPLFWSYIEDYLNEMRNQSA
                     AFHADAYNTAARTYIADNNARLHSGTLPATFTADELIRKGYLKGLNRSPFGQSYTTGI
                     RRNTSTGRLEALTCSTGGENIKDDALRSIASLLPGLGGFIGKNGTATGVFGGWTDKPG
                     DYGLSCNGGHIAIVMMGDDLQESDRLYRFQVPGRPELNQMNTAINMGGNNLNNAGNVN
                     GQSATLKGDVTSENGWLITKNDKGWKNITYGGGFTMTDSQWIRAVGGKGIITTGEIKG
                     GKVSGGTVRSDGRLSTGEYLQLDKTAVANTKCSPDGLVGRDSKGAILSCQSGVWVGFE
                     SYPVGSPIPWPSATPPQGYLLMNGQSFSCSRYPQLARAYPSCKLPDLRGVFIRGWDNG
                     KGLDGDRNRQLLSYQADQSGVYHERGGWLKGHHSGMPYWAQGSTTEMRPKNIAFNYIV
                     KAS"
     gene            22975..23169
                     /locus_tag="ACHURS_000291"
                     /pseudo
     CDS             22975..23169
                     /locus_tag="ACHURS_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000486721.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            23334..24155
                     /locus_tag="ACHURS_000292"
     CDS             23334..24155
                     /locus_tag="ACHURS_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HFR1236119.1"
                     /translation="MKTDSTGIAARMMLSLDRERICECLLSHRQLQSTPLQVRYPQGV
                     RDALGIMSEQLSLSVSDLTRILVEDALSEMFLPADNIVRRLLSRMEHIMQAHDISATT
                     MAALLAPWNIRPAVFREPDRLTDYLTGEILAALADWFYLSPEWLNGRVHYPLYRPGDW
                     PATQEIFCRIISARENMDIILWHGFPFAGTHSGEYCGVLLRQKKEINNTIIYPVLSLY
                     PARMDIEKEGWFQMARKISPDIPVRAVTLTPAQAEYLITGKILPTALFRVPLSPW"
     gene            24257..25459
                     /locus_tag="ACHURS_000293"
     CDS             24257..25459
                     /locus_tag="ACHURS_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HFR1236120.1"
                     /translation="MMMKKHITRTLIASAVLFSFNSTAATSYFEARNDAMGGTGVASS
                     HYGVAPLANPALLTKSGAKDDFSLLLPSVGAQLSDPGNIADNADKISDDWKAFDRAVD
                     SHSGVPQTAARLKERLQDFRNTHASAQAGVSAVAALPGDTLAAALMLKTLGTVSVDGK
                     VSDADLNYLESIADSGSQDVDKNRLTSQAFARAALITDVGVALATELETAGQKWSLGF
                     TPKFQRVDLFNYNTLIKNYDSSAFKGDRYHNTQNGINADIGASMDLDDNWTLGLVAQN
                     LIPRSIETKEVHGVTETFRIRPQVTTGVSWHNDMFTTALDVDLTPASGFTSDSKRQFA
                     AVGAEFNAWKWAQLRTGYRQNLASNNGSAFTAGIGLSPFDVIHIDVAGLVGTDNNYGA
                     IAQLQFTF"
     gene            25563..26021
                     /locus_tag="ACHURS_000294"
     CDS             25563..26021
                     /locus_tag="ACHURS_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238171.1"
                     /note="Members of this family include DotD of type IVB
                     secretion systems and TraH of plasmid conjugative plasmid
                     systems, both lipoproteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotD/TraH family lipoprotein"
                     /protein_id="HFR1236121.1"
                     /translation="MQKKRITLAGGVAMLCLSGCHLPPDNTAPARKYGAASEVAVTRH
                     AQYQLWEQGTYSAALPSRQPGGSLQAHSGRVSFDWEGDALELLSELARLRGQQFSYSG
                     VRLPLPVSLHVRDMTFADALRLTEAQTAWRAVLSQSAGRLHLSYMPTEKK"
     gene            26018..26854
                     /locus_tag="ACHURS_000295"
     CDS             26018..26854
                     /locus_tag="ACHURS_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000745049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HFR1236122.1"
                     /translation="MKKQVCLLLSGLLLAGCAGQHHRPPAGMAGNLPPPDLRAWLTPT
                     DNQDDGISDTRRQMLTDAGRTLGFRGGKAQRSWELIQALNARESTLNALYDFRPLISR
                     EGWLPPVIDEAQDVAHIQPDQIRTASRVWTILRPERFVSNPPGWRDWLLRGLSTTATP
                     GTEGRVVPEDRAQRRLWENALRQGWQEGRDNADLTLEANQKRLTRDYRGMMLYALLWR
                     QGMITRPDVTEQRQTVTGNGRKLITGDHVRRLKTHAEFTLQKSRWRPVVSTEGAPDEI
                     TR"
     gene            26838..27986
                     /gene="traJ"
                     /locus_tag="ACHURS_000296"
     CDS             26838..27986
                     /gene="traJ"
                     /locus_tag="ACHURS_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer ATPase TraJ"
                     /protein_id="HFR1236123.1"
                     /translation="MKLPDEFGTFPFARFTAEEFRYFFAWCARHRVSDIDLTGGSPVS
                     VSRFGRRVRSSAHPLPSSIMMALSDELFGREVLPRVLSGHPVDRTIQVSGDASGRYGL
                     QRGQRVRLRCHLIQGTSATEEKVISVTMRVIPSDIPDILTMNIEPDLLDAMLRKTGLG
                     FICGETGSGKSTLAAALYRYIQTHFPDRKTVTYEDPVEYILGGENDLLPPHQAEIGRD
                     VESFAAGLRSAVRRNPDIIGIGEIRDYETADAAVQAGNTGHFCIGTMHTKSPGETFAR
                     LLGLFPPEIRDSMAAATLSLVQFILVQVLVRTNDGGRQAVREYIVITDELRDTLSRQS
                     HATWGHYIDEIIRKEKRRIRDQVLTMYQTGSIEASEAALFIPAGEFPK"
     gene            27983..28273
                     /gene="icmT"
                     /locus_tag="ACHURS_000297"
     CDS             27983..28273
                     /gene="icmT"
                     /locus_tag="ACHURS_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IcmT/TraK family protein"
                     /protein_id="HFR1236124.1"
                     /translation="MKREGFWRDVTPPLNIYGVPLPFTMIYLLLFPYPSKTTFWVCSG
                     ILLFFVLLGYSGWTVRTLCRRLYSLVQGKQASGRPWWYRHRTELPREWRGTE"
     gene            28337..32398
                     /locus_tag="ACHURS_000298"
     CDS             28337..32398
                     /locus_tag="ACHURS_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD7 domain-containing protein"
                     /protein_id="HFR1236125.1"
                     /translation="MKSRYRTWLAVPPEETEAVKNAVPPLNGRKAVAWDPEKKLWYAR
                     AGTELSLLERWLPRPQELSMDAGDPVTEFAQVLENAGLVIQGLPQMDGAIHRVATRDD
                     KKGAKSGAYRAYLDGRPAGWYRDYRSADDSPTNWVFSGGEQHDPLARLHLRAFAQQQR
                     DDNARKLQQQYNKQAGYARSYINRLPQATAHEYLTRKGIRAAPGVRLNNKNELVIPFS
                     NGRGEIRSYQRIPVTGGKDARILKDSEKTGNWFTFGTPENGRPLLFAEGYATAASLHE
                     ATGLPVLMTVDASNMIAVAENARQIWTDSPFVFCADNDHQREINKGVFSATKAAEVTN
                     GEVIIPAFTEAEKAQGLTDFNDLDASRGRDNFQNAMNAQLKHIGIPTPNSDTADHREA
                     VVIDNLIFTPVKDEKPQMSPENRQSTAPETELATQDTPYDTHSDDTENQAYEDDYAAY
                     ASLQSVTAETLSPDKPLENIASAADTGTENTSVPTAGDQEDLNTDATGTDTAHNSDTA
                     ETGTLPEPAGMQSVQQATTEPTAEDKPHENVVSAADTREENASVATADTGEENASATT
                     ADNQSTQAEEHPRSKDTPETSLSDAAGTQHTTTTQSEEQVQVSMASADDWSAFAEDLN
                     ADTPGALTVHNTDNAETNTVSEPAGMRPVQQVTTELTAEDKAQEKSVASAAETGAEHS
                     SDITDDNRNAPAEELNADATVAVHNTGTTETASPSEPAETQPVRQATAEPTAEDKLPE
                     ERLVSATETSTANEAARPETPSLFSRLRRFLTGVKSRTSEPSVIQEKKPTEAETATEE
                     VAPPSPPETSPEDTSSAEPDTIIFAPRRPDSPQRHNLDEILKNLTHKEFPDRTALYSV
                     DGEPAFLDRLYCLEMVDGASADDKKVLAALAVATNFYGGVIELTGSDAFKQKAMQLIV
                     EYDIKVRMKLPAQRAALEKIRKEKEMAHDAIVTHRPTPELNRQSPEETTMAQPAPPVS
                     PKAEAEQTAGSTAETPHPEAPVTAKAEAPRADRPEQPAAPSSAPEKAPVSAQPQQPDT
                     PEEEPPGKLSPGESVTAVLTNYGEKEYAPGKGRCFFVELTNRSGKREYWGNGLEELVK
                     NHQKGDPVTLTLQARETFRLSGETRDRIRNIWAITPVSNGITVNNDQPHAGQHTLEFP
                     MATFSQVMLQMQQHWPQIMQEVRSPKNPPNNLYIGEDRQPASAPQDQSMRLPLAGNAP
                     NELIPAIASADKDNRQLNLLLVHSADDHLQGVVRLNGTLYPALATPSADNRQLVINAL
                     TDNGLQFAGYGEAVNHDENNHQRPSPQIMQFHLKQQDSPLFAAIHKPEEQPDKLFRSL
                     GFEQTWKEWSDSQKAEDRQEKTLQQAQSHSPGR"
     gene            32415..32765
                     /locus_tag="ACHURS_000299"
     CDS             32415..32765
                     /locus_tag="ACHURS_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFR1236126.1"
                     /translation="MNMKKTLLVILTGITLASPVAGAATCRAGAAAEAGSRAAWERAR
                     TASQAWSQRENDVASSLQSCLSRIKDISITLPQFPSLDKLLRDLENQVCDAVVDRVND
                     HLPGNIDPWKDYNL"
     gene            32777..33472
                     /locus_tag="ACHURS_000300"
     CDS             32777..33472
                     /locus_tag="ACHURS_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HFR1236127.1"
                     /translation="MSEAQPTSPVSPEADNSGDIYNPVLRTIAQSEQRAALVPGLVRA
                     LLYSGAALCISLVANGIQYWHATGVTREYFATDNGRLVRLAPTSQPAWSQSDVMNFGS
                     MTLSEAFNLDFVHYRKQITTQAPRFSEAGFAGYVNALQASNILDTIKKEKFNLTTTIG
                     AGTLVNQGQMENGVWFWKFQYPVRMRLVGQTSTRPEQAFTFEITVRRVDPRIKPGGME
                     ISQMVSRNAAAGI"
     gene            33483..34457
                     /locus_tag="ACHURS_000301"
     CDS             33483..34457
                     /locus_tag="ACHURS_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotH/IcmK family type IV secretion protein"
                     /protein_id="HFR1236128.1"
                     /translation="MHIHHLIGALLIVCGTVTAADNPGWKNARSPQTGGKTAQATGNT
                     PAGGPPPAAQLPGNLPPPGAALVQEGASLDSPLNASEIRELRGIMADNERAISAPLTS
                     VVPRISSLTVNLSPGASLPLVRTALHNVSVVTFTDVNGSPWPQSDPPYNAAPGHFDVQ
                     FNGNMVSVTPLRAWAAGNVTVYLKGLSVPVILNITSGETDTTTLSQEIDSRLDLRIPR
                     RGPASPVVSIPSEKIALHDATLQAFLDGVPPDDPAIRRLKFSGNVPDTTIWQHGDDLL
                     VRSRAMLRDEFEQTLASADGTRLWKLPVTPLLTFSVNGQSVHVTPELE"
     gene            34461..35798
                     /gene="traO"
                     /locus_tag="ACHURS_000302"
     CDS             34461..35798
                     /gene="traO"
                     /locus_tag="ACHURS_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /protein_id="HFR1236129.1"
                     /translation="MSAEQEQDTAAKSGKKLSALLVMGGVIIIGGGYLLLTWLSPENA
                     ASLSSVNINSTTSGGGRNVMETQHYRDLLRADNERGAAEALRNNETFIASLPRGLDTD
                     TGNDQKRDNPPDDQSGTSGQNASLPQEDQTAREKRREALQKLLARINTQHQDARPPVV
                     AAAMWSTTGTPGTAPHASPSGTVTSQHPRLQETSLATPATRQGIQLIPALTRVPAYID
                     TEVDSDNAVSRVVASVPAGPWAGAVLYSQDARLAGQGMDIHFDRMMWRGMFLKVNAYA
                     LNERTGMSTVASDVNHRWFKHIVLPSVLGGVGNVGSLYKDANTQILQGNYGSITGRVG
                     MPDGEVLAGVIAGGMAERGSQLLTRQAESEPYRQVTVSRGEVVSILFVEPVMSNDIST
                     PSSGTAPSESPSSGRPLTQAEAEHQASDRIRAAVLRKQNEMQQRYGIQQESYP"
     gene            35795..36508
                     /gene="traP"
                     /locus_tag="ACHURS_000303"
     CDS             35795..36508
                     /gene="traP"
                     /locus_tag="ACHURS_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HFR1236130.1"
                     /translation="MKNEHEDPGSFGETTAPASGKSFLKRQYMGYSLPVWAGCALIIL
                     VAIWYKFLRTPSEPPADAGFAVTPPAAESWAEPMTMTESGIGNNMPAPPVTPQADMQQ
                     LARDLKAELDRRDEEIRRTLTMLKDSITTLSNAIQKDEAWAQETRQLLEETRKQVHTL
                     QMQGALPAPTGNNRQPAKTLPSPLQGMKIMSVEKGMAWIRWQGSAWAVREGDSLGNIT
                     ITRIDPSSRAVITSAGTLR"
     gene            36505..37035
                     /gene="traQ"
                     /locus_tag="ACHURS_000304"
     CDS             36505..37035
                     /gene="traQ"
                     /locus_tag="ACHURS_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraQ"
                     /protein_id="HFR1236131.1"
                     /translation="MMSIDALTAIENFASGIFSAGMEFLFTWGELLGIIGLIGHLMRA
                     RAEGRHSMGPGKFIAGIFICGMLVSLPSMINAGGTQMGFRADSFAPIAYVQPGSFGAA
                     AGAVNAILSLAKLAGVGFVMNGISIWRKSLLDGHTALSASESISKGNVKFVAGVLLVF
                     NDRVIDATLASLGIAF"
     gene            37082..37480
                     /locus_tag="ACHURS_000305"
     CDS             37082..37480
                     /locus_tag="ACHURS_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6750 family protein"
                     /protein_id="HFR1236132.1"
                     /translation="MSRLRAMAARLRAASVYVQLCLMACRDRLVRLLLLLPVMLLPHT
                     AMANTLFDMIKTVGDGARGSSSSFVAIGQVIGLVLFIGGLLGLKQIGKKPGAGLACLL
                     SILVGVCLAVVPELMGSGQKTLGLSPVSIS"
     gene            37537..37788
                     /locus_tag="ACHURS_000306"
     CDS             37537..37788
                     /locus_tag="ACHURS_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003665769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraS"
                     /protein_id="HFR1236133.1"
                     /translation="MSCELPPFTLLRTEHGNVIARDNRDNQEFVFAECYSTAIAALIL
                     AAVTLYTERKAYEKDPATHRPGPGTMAFPAPATGHGIQR"
     gene            37703..38521
                     /locus_tag="ACHURS_000307"
     CDS             37703..38521
                     /locus_tag="ACHURS_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraT"
                     /protein_id="HFR1236134.1"
                     /translation="MKKTPPLIAPVRERWLFPLPQPDTVFSGEALLPPSVLSTEGRCP
                     CCRRTVTHRFILEDSWPLQQMADTCRDTVVLLEKNLTRVMRLKKRPVPENADEKKKHT
                     RTLQDAERSLAQARLSARRLALRHVEKSQIVTTDALSENESDLLQPEGPPFHLCAFCH
                     AWHCLNGYAAAQGVMVWLPDLHPASVVALNARALQEIFSDNRQRVRQGRAVLNALVQN
                     RLAVEEKFRTWRPADFADALRRWPPAQRKTLREKMDGVALILLPDSFPDKKYVM"
     gene            38816..41860
                     /locus_tag="ACHURS_000308"
     CDS             38816..41860
                     /locus_tag="ACHURS_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR1236135.1"
                     /translation="MNINRIIFAIEDCISTLSRYSVSAFFPDYCDLHTVIGLDQQDRE
                     RRPWLKAPYIATTKQGYYLSVFEISGALKEMDENPDQTAPGTLESLIAALAERMNTAW
                     KNSGHKISFVFERDPERGRDEIEAMLLPQRKSIARTGILLQDVLDEKVTTLTPWLVRE
                     RCWLTVWSSEELLSRTDRKDHQTRVRKLAEHAPPARFAQDPWRWTLSALKIRHDALLD
                     TLEQALTHDSDGLLIRLMDIHEVGREIRRQLERNSTPAVWQPHLPEDARPAGWRQGGD
                     TSVFHAPSLNLQLFTTQPETHGSLIQAGELWHGMVAITLPPQNLRTFNQLVRDVPRAI
                     PWRIRMDLMPDGMKALGVKKTLLTYSSFIPPLRPMYDSVMMLNDINKHDPVCIMTIVA
                     TTWGNSREVCTRNQALLKSALEGWGVCGTTTTFGDPRRAWVNTVLAASHSSGPIPLYP
                     PLSHALSLFPLNRAGSVWRGQGNLMMHTEDGSAWEVALASSQQNKHTELTPGAPGLGK
                     SVLINALSEIQIASAQKNLPFIAYIDKGFSAQGLVQLIRDSLPEQRKDEAVGIILSND
                     PDHTRNLFDVMYGARKPVTPEKNFMVSVLCALCVDTGTGQPCNPGDTRQIISSLVDLA
                     FREYGENNPRLYRAGTEPLVDLALEESGIAEQHDAGWWNAATWFEIRDMLHIAGNIPA
                     AQRAHYQAMPLLAEMSALLGQPSIRDVFGTVQRDNSEERLLDYIRRALDQGHSDYPMM
                     SGCTRFMLSPDTRVVAVDLNNVAGDKTPAGRLRTGIMYLLAGQIAGGDFVLPQYQEEI
                     RKNLDPRYHEVIFRRIEQLDQEVKTKVYDELHNAKGINFIWEALDTQELEQRKFGIRT
                     VLSTQLLQHYPPAVLKSANTLWLLRYRPDEIPFLRDNFGVPEVTLRRFLKMPEGAAPD
                     GSGVPVLAVFRVKNGTLARILKFTLGPLELWALNSSPKDSALRRALTQEVGSLRARQI
                     LAEHFPRGSATSLIEHRARTHDSENVIHELAAELIRKQGYNL"
     gene            41860..42480
                     /locus_tag="ACHURS_000309"
     CDS             41860..42480
                     /locus_tag="ACHURS_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraV"
                     /protein_id="HFR1236136.1"
                     /translation="MYSIHITPRCRRPARGQVFLKTLRVDGDSFSFTLPSACFTLRPS
                     PNPRVIFASDRRADGEMSGIRLALPGETGTLITTATWQVNVPERPPFDIQHVIHWLLP
                     APLTALMSDNTWLWPDVPGTPALTAGDWPVMESDTPTLKRVKRRTFTLIDNRNRQGWI
                     TRRFQCFPLAPLPESLSHRVPDILYPPKTEKKECKDNLQPHCLHSL"
     gene            42438..43643
                     /gene="traW"
                     /locus_tag="ACHURS_000310"
     CDS             42438..43643
                     /gene="traW"
                     /locus_tag="ACHURS_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraW"
                     /protein_id="HFR1236137.1"
                     /translation="MQRQSATTLLALLVGCAVIPATHAYSVNVVSSQPVTRQIMPELA
                     TIQGIMEEMVRMQTATGTAITQNSEKLATVIAQDGQATRQQMIFSNETHRLEEARKSF
                     SVPDSICSESASGIAAESRRAAASAAARLSQGGGVSSKPIRERLSRAADSPVREAYDS
                     AGIHAGYCTEAEYARFGGTDVCPAVGDLPGGDSQVRSLYQGAGTADTPAALTWDQKQI
                     DAATAYMKNTARPSAGRAPGKGEVGTQTGRTYVGLQNEYNGIIDAASHPQLSLIADST
                     PNEATRGALTEALQSPSAAAYFDRTASSEARTRGHMSQREFETFEAGRRYANTDWQQD
                     LQGMEGDNLLRELLRTTALLNWQMNDLKEQIRQGNVIAGQQLALAARQYYGQRLGELS
                     QAMSQGSVR"
     gene            43640..44209
                     /gene="traX"
                     /locus_tag="ACHURS_000311"
     CDS             43640..44209
                     /gene="traX"
                     /locus_tag="ACHURS_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000650756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HFR1236138.1"
                     /translation="MKDDNSIDEKKPAPRLRRIGDIGINLFAPLAETRWMLRSCRHML
                     DQHKTRLKRLMPDRQPESPVCMTWEEAVRASGLTVDELDRRLRRRRMFWRTGFWLMMI
                     PVLLLCGMALAATALPVITLIRILVCLLLMSTIAALCASKALIASYRLWQLNERRVSE
                     DEQGTFPDYLQARHGWKQATWLAITHRQY"
     gene            44284..46449
                     /locus_tag="ACHURS_000312"
     CDS             44284..46449
                     /locus_tag="ACHURS_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HFR1236139.1"
                     /translation="MKIIPRALSAGLALAAVPAQAFVTYQDIVSAATNPDDLSRQALV
                     TIFGDVVTNPLTASSNTIIGNLFMVFNAIIATMGIVWFLFIGIRHAVRTGHQGSVFNN
                     GRDVVGILSVVSGFLMIVPTASGWSLAQLVMLWGASIMGIGSANIMVQTAAENIAEGY
                     SMTVQPVHVSTRTAARGILEMELCKHAMNTGLDDFNRTARSSTPPMSESSRTENGRYT
                     VTVSNGSATCGSVSLSVKGNGSNKQSALAHFFNPFIETEYQGVLTAQRSAMDTMLRDL
                     DSAAQEFVSAYRTRRDTGNGNLPDIESRIQKAANRYEETVQKALPTDNAETERREQLK
                     SYLTTYGWVALGAWYQTFATANQRLAELADRAPTVSGVSSLGEAGDTEFLKEVKGAYQ
                     AQLQNSAYTPALGTTTSASEYHASNTQNPQSALLEFIGPGLTRFSNKVATIHSGTGTT
                     SQQANPLLKMKDIGDYTMVTTETIWAGYTTVRVLSTAGSNSVFGRLVNAVSGLSDAAK
                     AFLEASAPPLYFLLFLLFCAGFSLSIYLPFIPFIFWMTGIGNWIVSVLIGCAAGPLWA
                     ATHLGTSQDRGSRAAYGYIYLIDSMIRPLLMVFGFFFASVAIVAGGTILNALFAAALM
                     NVQINSVTGVFSMAGFLMIYARACTTLVAAIFALQAYLPDHVINFLGGRDGVNMLGNM
                     VSSVKDIIAGGNTNMRRTPGVREDRLKNINRDDKDGIKQ"
     gene            46538..47188
                     /gene="excA"
                     /locus_tag="ACHURS_000313"
     CDS             46538..47188
                     /gene="excA"
                     /locus_tag="ACHURS_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid IncI1-type surface exclusion protein
                     ExcA"
                     /protein_id="HFR1236140.1"
                     /translation="MNKTKSRYPSWWAYYHLARNAYFLLGIPCLLLYSAAYTSDLLDD
                     NLRHSISLFILAYGCYFLLFIPPLWLYLRTRAKKKKVQQVVQQIKESSPFAPTSDYEQ
                     LSFSKSCYFGIDIKNGTMLYVRIYPNNVMDVIGLDIHNFTRTVAEDGKLEIHTTYVSL
                     PMIPLEISGISARTLANTMHTMAARGYEYKERFPQMIRYRVKEWEKVAGVPVAEVF"
     gene            47462..49048
                     /locus_tag="ACHURS_000314"
     CDS             47462..49048
                     /locus_tag="ACHURS_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsA endonuclease N-terminal domain-containing
                     protein"
                     /protein_id="HFR1236141.1"
                     /translation="MSESRSNFPARHGTPWNTEELQRLLVSIAEGVSLKKLTQTHQRS
                     SGGIRGAIKRLLPSEFLEFNDINTVNDLSNYFNKIPRQEHNSIINNLISLSTAKTIAH
                     DKVEIQKNSFKQNTPLPLCQPNTDFKDYIADEHDVIMLIHSAIESLSNKRHQYILLMR
                     LGTDDSPHSLADIAVKLGISRERVRQLQERALGLLAAKSRYEGTPGACLKALINSIGN
                     SPYDIAVWIYRIVHRDFVTSSDLAAKFIIFTAGIPKARRDEIKNSLSLISQLQKQKLR
                     ERRAELAHAHAQERARIKKERVEFIFSKWLDNTHWPKYIAPPPPVEQLCSQRAVNDTD
                     ISGFFYSQKLGRTVQYESGLEFKIMNLLECCDQVLFYQEQPFSIPYCFKNKSKKYYPD
                     LLIATVDGRCLLMEVKPTDRMTLSLTRIKSNAGRKWAHTRGWGWLVISDRFSLRQLEE
                     HAISTNTWKLIEAELKTSRILAWREMMELRTRYEIHRLDLIAYIIQSGAEVDNFYRIT
                     PKISNNTSNQRQPNESDCHN"
     gene            complement(49186..49338)
                     /locus_tag="ACHURS_000315"
     CDS             complement(49186..49338)
                     /locus_tag="ACHURS_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR1236142.1"
                     /translation="MPQRTFLMMLIVVCVTILCFVWMVRDSLCGFRIEQGNTVFVATL
                     AYEVKR"
     gene            49668..>49871
                     /locus_tag="ACHURS_000316"
     CDS             49668..>49871
                     /locus_tag="ACHURS_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1236143.1"
                     /translation="MKDEIHSTSINPDVVEQFLALMNTHCKSSEEALTMLSTLCYNLM
                     MTCGIHSMDSQMLNGHGISMQLQG"
BASE COUNT        12480 a        13725 c        12648 g        11018 t
ORIGIN      
        1 aatgtatttt aggatgacct atgttgaata gaacctaaaa caaaacaaat attaataggg
       61 gtccgcttgg aaaagggaaa ttttcccacg ctaagagaat tgagttgaac gagtggttac
      121 atccaggcca gctaactcac tgaaccccgt acagggtcag acattcggta tctccttcat
      181 cctctgagga aaacaaatga attaactgct tacgtatatg gatacatata cagtattaat
      241 ttattaagga gaaaatggca tgtcaagatt taatgatagt gattatcatt aacatgaagt
      301 aatgttacac cgttcagaaa gcctgctgac gctgtggcag catcacagtc tgagtattct
      361 ctggtgcaat acaaaaaccg gactttcccg taacaacaga caatgttacg ggaaaagtta
      421 cagaactgag ccacaaaata tctcagtacc tgagatgcat aaaaacaccg tacatatgtt
      481 tctcaaaagg gggatgatcg ttatccagcc atttcggtgg atcggtatgc agaacaccaa
      541 tctgcttcat gccggtatgc tgcctggatt ttctcgcgca gtaatcactg tcttcatatc
      601 ccatcgtttc gatgaccact ctggccgtct ttccgtcagg agcccttgca gtgacaatga
      661 aatccggcag gacatatttc ttttcaccat taacataaac ttctgtatca aacaggggcc
      721 tttccagact taattccggc gtacctggct ttcttttaag ccagctggcc acattcagaa
      781 gagcagtgag cgtgtttcgt tccagtttgc tgtccacagg caccggacat gtcagttgat
      841 aaagagcatg agcataccct tcactgcaga tgattctgcc atcggcactc cggcaaaaag
      901 caagaataac ccagtaaggg ggacgtattc cgccctgcgc ttcaccatta atactgatcc
      961 cccgcacagg tctgatgacc tgaatattac cgttcgccca gtgaatttcc acagcatcac
     1021 gacagatgtg ctgcgccata aaaatctgaa agaacatatg ttttcttctg gcaggccagt
     1081 gtcgttctga attctccagc gtttccatta accgctcctg agacctttca gacattccag
     1141 gccggaaatg gataatttca gataaggatc ttccccgaat atactcctgc tgcagtgtaa
     1201 cctgccgtat ggcatcaaga cactcacgat aattagtccg gatacgggga ttagccaggg
     1261 aatccagttt attcagccca gcatcgtcaa taagcgacag gagtaaacgg gcaaggcgcg
     1321 gacgtttctt gcgaacggag gagctctcac cattatggta agcagagcgg gcctgcagtt
     1381 tgataacaga attactgtca tcaggtggta aaaagtttct gtaatttata cgggtgctaa
     1441 cgggatgccg gcttgctccg gaagatgttt cctcttcatt tccggataac tgacgataca
     1501 tggggcattc aggtgcatgc tcatgattaa aactggacag ataaagagta cccgtattgt
     1561 ttttaagagc agcaaaattc atggccggcg aatctcccgg aatacagtca cactgtaacc
     1621 atgcatcata ctcacgcatt tcccggagaa tatcattggc caggttatgt agtgccatgg
     1681 ttgctggctt tttcagtcca cagaccgtct ttactgcagt ctcttcctca ctgctaagct
     1741 cccgaatcac tttgccccgg ggattcttct ttacaagtat cattggcata atgaaagcac
     1801 tccttatgta catgcttaaa cagtaagtaa aatatacaaa gaacacccca tgaaacaaag
     1861 gcagagatga actgtgcgcc ccgatatctt cttctgatat cctttcaaat gaattgtttt
     1921 tatttatatc atgacaacat taaagtgaga gaatatatca accatgtttc tgtcagggtt
     1981 cagtagaata tcaattcgac tgtcacgcca taactgttac tacctcgagt ataaatactc
     2041 agagaacgga cttattagcc atgaaaaatc acctgatggg aaatttacta atttatgaat
     2101 tgtgatatta ttaaagagta aaaccagcca gccgaggtat ttatgggaaa aaatcaggaa
     2161 gaaaggaaaa ctcccgtcat tgtggtgaaa aaacgtcgta ctttttcgcc accgtcctta
     2221 tccgaaaaaa cagatattat cgcacctgta tttactgaac agacggcaga atcagccccc
     2281 gccggaataa attcatctgc cgtcgaaaca catatccccg aagcgccagc caggaagaag
     2341 aaaaagaaaa gacaccgttt tccacgtccc tcccactgga cccgtgagta tactcacgag
     2401 tgcgtggaaa aaataaaggc cctgtttcct catcttcgtg ctgaaggtgg tggatttatt
     2461 cccctgaaaa ttggcataaa caatgatatt tcggcatttc tggcagaaca cccggagaca
     2521 gaactgacca tggacgaatg gctctgtgca gtgtcctgca tcacatcccg ccgcgtatat
     2581 ctgcagcgga cagctgtcgc cggcgtccca cgttatggcc tggatggtca ccccaaaggg
     2641 caggtctcag actctgaagc acagagtgcc ggtcgcagac tggcgacatt agaacaaaag
     2701 tggctcagga tgcaggcaca gcaggagaat atttctgggc aataaacact cctgataaca
     2761 ggtggacatt acgtatcaga aaaaatggga agagaatgtt atgtgtgatt cagaagcgga
     2821 gaagatttta ttcatcctga ataaacatga acgggcatac agatgccagt accccgtata
     2881 acttgataac atcaggaatt ttattgataa acagcagtta acgaatagtg atactccgaa
     2941 gaaaagcacg gtcccaaatg aagataaaaa aggcataaga tgatgatcac aggaaatgga
     3001 ataaacactg taactgtcaa tggaaaagtg aaacacatca cagaactgga tgatataaca
     3061 ctttgtttag agtggacaaa actacgagag gaaaacaatc gtctttatga aattaataac
     3121 caggcaaaca gaggatggcg tggtttcatc ctaagattga taggggttaa cctgccagat
     3181 aaaaggactg agtttacaca acgtctctta ttgacgcgca aaatttcggg ttcggtcatg
     3241 aaaaagtaac atattctatg ctggaacaga ccaattttgc attcgccgtc caggagtttg
     3301 aggggaaagt agtcagtgtt tacgctcagt cagggaaagt gtgcaatgat aaacaacacg
     3361 aaaggaatcg tagtacatgg gcaccgcgat tcaatattca aggaaaacac aatacagcaa
     3421 tgtacttgta ctgtttctat tattctggcg atttcaataa aggcacttgc tcgcgctctg
     3481 tcattttctg aaactcttca tgctgcattt cgattttcct tctaagatca taactcgcat
     3541 acctgttctt tttatgtata tctatatcta tttttgtgac gagttcaaac cacaatgttc
     3601 gcactcccca ggcagcgtta tgaaacattt ccgcaacggc tatagagtct gtattaactg
     3661 cttcggtcat gtagtcgcta atacgttcag cggtaatttt tgttatttca tcgtaattca
     3721 ttttatctcc tacaagatta tttaatggaa tgaaaaatat ctggcctgat aataatattc
     3781 cagaattaat atctgcaggc tatgttaagt tgtccccaca cacaatctac ctgttttctc
     3841 gtgtgtgcca caggcgcaat acataaatag tctggccatt aagttcgtaa cggatttcgt
     3901 actcgccagc aaaaatccgt ctgacctccc tgggttcaaa ctgaaacaac tgttctccca
     3961 tacgtggatg agttaacaga atgaccggtg cctgtgtcag ggattgcacc gttctggcgg
     4021 ccgcaggttt actggccagc accagaaaat catataaccg cgccaaatca gaaagcgcct
     4081 tactggtcca cttcagttcc atcagcgtgg caccggtaac ggattgtcag tactgaggct
     4141 gtccgcccag gcctgtacag cctgatggtc gataacctgt ccggatgtca catcgtccag
     4201 ggcttccagc gtcaggcgat tacgctcctc ctcctgggca agccatgcag aaagcgcctg
     4261 tttgataacc cagccccggg agcgttccag cctggcggcc atctggtcga ctttatcagc
     4321 cataggtaat ggcacatgcg cggtaatgac ttttgttgcc tgtgcggtat tgtttttcat
     4381 ttgcatatcc gattagttgt taatcatatt taatcatagt gattactttc agacgaaaca
     4441 aggcaaataa tgcgcgtcag cgcatgtaag gcggcatacg gtggttacat gtagcacagg
     4501 tgtgaaaccg ccgaagcccg gcgtaagccg gtactgattg atagatttca ccttacccat
     4561 ccccagccct gctaagccat acccgttttc agccatgaca gagcttctgt gcgcggtcgg
     4621 agtggtcccg acgagggttt acccgaagtc ggggcgtatc tccgcgttag cgggccgtca
     4681 gggccgctta cgagcgtgta ttcaacactt ccagcgagaa gactgacagc gatgatgtca
     4741 tttagttaca acattcataa ttaaaagcga tgttgttccc gcccttcggg cgggggcggc
     4801 ctcgcttttc agttatgagg ggtaggcacg gtgctgcgtt tgtgtggtgt ttttattcct
     4861 gatgtctttt tgttgtggtt tcagaacggt gaggttcggg gcccgcgcgt cagcgtggga
     4921 ggtgctttta gattctgagc ggcgcggcct tggccgctgc aagcgaaggc ctctttgtat
     4981 ttgttcacag tggtttcaga gataaagact ttccctgcgg cgaagcaacg caggggatta
     5041 aatgatgggg ttaagaatat attgcaggtt tgctgtttca ggtttgcagc ttcttaaagt
     5101 tctttgtttg cggatttatg tggataactg aaacaacgtg gccacgttct gatgactgat
     5161 gcgtggtttt atgggcagat atgcccctct gaggggattt aaggtgacta ctaaggattt
     5221 tgggctgagc ggaatctgcg ccgaaagccc cggaggggct ggcgatgatt cagtgctgag
     5281 ggaatgatgc tgtctagtgc ggtggcgggg cagccagcgt cagttcaagc tgacgcaact
     5341 ccctgatggc cagtcgcttc agatggtcat cggaacagaa ataggcttca tccggcggca
     5401 gacgcttccg gagatactca gccatctcat aaatctgctg gtcaacaggt aatttcttca
     5461 ggcggttggc acgcttgctg gctgcggctt tttctcggcg cttcttcagg gcatcctggc
     5521 tgcgctgcag ataccagcgt tttctggccg catgaacgga gatatcttca tcctcacgca
     5581 gaacaccttc ccgaatgagc tgctggcgga tttcactttc acgcagtcgt ttctgctgct
     5641 gttcgtgaag ttttaccatg tcgacgccca gcatctgcca gcctgccggt gttatccaga
     5701 cgtaacgtgg cagacgctga cgatgttcac ggtcccacat tgtttcctct gacaccccca
     5761 gcacaccaaa gcgcacctgt tcggccagca gacgggaaag acgggagacc gtcacttcca
     5821 gttccggaat aacatgtccc tcgctgtctt tcgggctgat ttcttcagcc agccgagtga
     5881 cgctcatccc cacggtatgt gtgccggcat cactgaagct gaccagaacc ggccagatgg
     5941 catcgaggag acgttgtttt tccggccgga aagcgtattt gcgaccgacg aacgtccgtt
     6001 tctgtacata aaaaggatgc cgcgacatgc gcatgcgcag gctctgctcg ccggtcaccg
     6061 ggtccttttt caccagacgg ttataggcgt gcccgagctg ccctttcagc gctttatagc
     6121 gctcaggacg ataccaggac ggattttcgc atttgggttt ggtggagtgc tcaccgcgac
     6181 ggcgcttcgt gcgcttcctt tccggttcaa ccaggaaagt ggtcgccctt cccgcttcat
     6241 agtcttccca gaaacggatc tgctcttcgg gtgctaactg actccagtgt actgcgttat
     6301 atgaagtatt cttcagacct gccacaagct gtcgcttatg gcggagcgat tgtgccgtgt
     6361 atttatacac tgcacagggt tgaatctctg gtacggtttc atatatactt atcccgtatt
     6421 ctgtgaggcc cccattattt ttctgcgttc cgccaagttc gaggaaaaat agtgggggtt
     6481 ttcctttatg gtcttctgaa acttttttca gccttccggc accggcaaac tgccgtcgtt
     6541 tctgccatac gtcctgaatg ccttatggcg cattctgccg ttttctggct ttccggaccg
     6601 cgaggatccc aaaagccact gaataacgat ccgcaatata ccccttttcc accgctttga
     6661 aaagccgaca cgatttctca gagttacagt tgccctcttt aaccccctaa aaagcgtcgt
     6721 ttttttgaac tctgtcaaaa aaatgaacaa tttcacaaac gatcttttat gctgcttttt
     6781 tcttcttttc cagtagaaga gatcgccgaa tttttttaaa cgatctaata tcccgcacac
     6841 aacatcaaat acagaataat attctgtctt atcaaatata acgatctatt caactgctta
     6901 tcttcgctct tgccacagat acatccttat gtgatatatt ttccagccat taatcctgtt
     6961 tgcaggagtt gtcggttttc gcccgcctcg aaaccgtcca caaggcttac aagagtgcgt
     7021 aaatcatgtg cctgtcagaa ctgcaggcca ggctgaacgt gggtcagccg ctgtttccga
     7081 cacatgcttt atgtattcgc cgttcacaac aacatcgaag cccccctgcg gggatcgatt
     7141 ttcgcggagc gttccggatt ttgaatatca gtgaattaca tcaacgcaac tggtatctgg
     7201 cacagtacat ccctgccggc aaaaaccgcg aacatctctt ttcctggctt tccgaacaac
     7261 acgtccttcc ctggacaccc ctgatactga aaaaggtccg ccgcacggat aaagtctgtg
     7321 gttaccgtcg acatatacac gccgtgtttc ccggttactt tttcctgaaa gcagaccccg
     7381 aaagccacag cttcactcac cttcgccgtc acagtgcctt tctggacttc gtgaaaatgg
     7441 ccggtgaaat caaaaccgtc agggaggata tcgttcagtc tttgatgaaa gtgtacccgg
     7501 atcctgcgct caatccggca gccagggagg agctggatgc tgcctccacc ctctggctga
     7561 caaaagcgcg ttaccagtac ctcctccggc ttgatgcaca gcctctgccg gagtcgagga
     7621 tcgccctgct cctgcacctg gtatctgatg acggagcgtt gacatgaaaa ttgctgtctt
     7681 aatttgacga cttttcccgg cctttatggc cgggattttt ttcccttctg tttccacaat
     7741 gcccggcacc ggtgacacct gagggtgtac ggagagcggc tgtcgggcat tttttcagga
     7801 cgcctttatg accattctat cccaggaaaa ccctgtcatt ctgttactgg agaatatgct
     7861 cgaagcactc tccaccgccc cggacaacat aaacaacgaa cggcgtcgcc ggcgttacct
     7921 gctgaactgg cttgacactg cccgtcagat gcgcgagttc agaggaatgg ctgaagagtt
     7981 cactaccctg cgaaaactgc tggccaccaa ttcagacata ccggtagcaa acaccctgaa
     8041 atcactgctg gaaagtggca ataacgcagc attatacgac ctgtaccgct tccgctcggc
     8101 tcttgccacg tgcaggagtc tgggatggca gattggcacg tgcgcatgga atgaccagtt
     8161 actgagtgag acactttccc gcgcacagac cgggaaacgt catatcctgc agctgaacag
     8221 caccggggac accttttcca ccacgggcag aatgatcaga ccggtggcat tttcattaat
     8281 ccatccggcc accatcaaca cgtctgaggt tgaagagata ttccgggatg aaggttttat
     8341 gctcgcacac gggcgcgaat gcagtctgaa cggcagcggc agaaatatgc tgtcacgcat
     8401 tcttcatgtg ggccattcag gtttagcgga agaggaatgg ggcatggaca acagtctgct
     8461 tttacactga aataccggga cagccacaac ggcagccact ctccgctgac tttttttcgc
     8521 aaatcgcggt ttgctgatgt ggatgtccca tttttgatta tccgtcctct ccggttgtca
     8581 taaagcgcat tccggcgtga ggcttctgtt gttatcatcc cctcctgcag acatcccctt
     8641 gtcttctgat gactgacgat atgcttcttc tgacaattaa agaagcggaa gcttcacgcc
     8701 ataatgttgt ttgactgacc tgccacagga ggaaaaatga cactgacgtt acttaccgcg
     8761 tataacgcac cctatctggc agccctgact tttgttctgc ttactggtat cgctgaactt
     8821 atcgccctgc tctgcggaca ctccctctct tcagctatgg atacacctga tttaccggag
     8881 ggtctgacag gagaggcgct ggactggctg aacatcggac ggatccccct gctgatcgtc
     8941 ctctgtatgc tggcaggttt ttttggtata tccggcattc tcctgcaggg tcttattatt
     9001 catctgctgc aggctcctgc tccgaacatt ctccttgcac cactttgcct gctgctgaca
     9061 tgtcctctcg tacaccgcac cggcagactg attgccccct ggttaccccg tgatgaaaca
     9121 acggcgctca ccgaggaggc attcatcggc tgtatggcca ccatcactgg ttatcacgca
     9181 gtggcgggtt ccccctgtga aggtcgcttc acagaccgct tcgggcaggt tcattacctg
     9241 ttgctggagc cggaggccgg gaaagaattc agacgaggag acaaagtgat tattgtctgc
     9301 cgtctttccg gttcccgctg gctgggcgaa ctgaatccct ggccggatgt tctttaatcc
     9361 cgacatatac agaggttctc actatgttta atcttccggg atggttgttt accgttatag
     9421 cgctggtcct caccctgttc gttatcgggc tgatatttgc ccgcctgtac cgccgggcat
     9481 cggcagagca ggcctttgtc cgcacaggtc tgggaggcca aaaagtggta atgagtggcg
     9541 gtgcaattgt tatgcccatt tttcacgaaa caattcccgt caacatgaac accctgaagc
     9601 tggaagtcag tcgtgcagcg gctgaaagtc tcataacccg cgaccgcatg cgtgttgatg
     9661 ttgccgttgc cttttttctt cgggtaaaac catcagccga aggaatatcc actgcagccc
     9721 agacgctggg acaacgcacg ctcacacctg aagatctgcg tagtctggtc gaggataaat
     9781 ttgttgatgc cctacgggcc acggcggcca gaatgagcat gcaggattta caggatgccc
     9841 gcgagaactt tgttcagggt gtgcagaaca ccgttgcgga agacttatcc aaaaacggtc
     9901 tggagcttga aagtgtgtcg ctgacaagtt tcaaccagac tgcacgggta cattttaatc
     9961 ctgacaatgc ctttgatgct gaaggcctga cccttctgac ccaggaaaca gaacgtcgtc
    10021 gtcgcgaacg caacgaagtt gagcaggatg ttgaagtggc catcagggaa aaaaaccggg
    10081 atgcactctc ccgacgtctg gagattgaac agcaggaagc cttcatgact ctggagcaac
    10141 aacaacgggt aaaaacccgt acggcagagc agagtgccag tattgctgct attgaagctg
    10201 aacgacgtcg tgaagcagaa tccgcgcgaa ttctggctga acgtaagata gaggaagccg
    10261 aaatcgaacg acagcagata gtgcgtaccc gccaagtcga agccgaacgg gaagtggcca
    10321 tacgggaaat tgagcaacag caggcaaccg aaatcgccag ccaggccaga gccattgccg
    10381 tggcggcaaa atcggaagag cagtcacagg cagaagcccg ggcaagtaaa gcactggctg
    10441 aggccgtaca ggcccagcag gatgtggaaa ccacccgcca gactgcggag gccgaccgta
    10501 ccaaacaggt tgccctgatt gctgctgctc aggaagctga aacgaaagcg gttgaactga
    10561 cggttcgcgc acaggctgaa aaagaagcag ccgaactcca ggcagcagca attattgaac
    10621 tggcggaggc cacccgtcag aaaggtctgg cagaagcaga agcacagcgc gcactgaacg
    10681 atgccattaa cgtgctttcg gatgagcaga ccagcctccg cttccgtctt gccctgctgg
    10741 atgcattacc tgccgtcata gagaagtcca cagaaccaat gaaatccatt gacggtatta
    10801 aaatcattca ggtggatggt ctgaatggcg gtgttacccg cggtaaaaca gcaacaaccg
    10861 acataagtgg cggaaatctc gctgaacagg cagtaaccgc cgctctggct taccgtacac
    10921 aggctccgct catcgactcc ctgctgaaag aggtgggttt atccggtgaa ggacttacag
    10981 ccctgacacg tccactggtc atgcctgccc cggaaaaggt tccggatgtc agcgaaccgg
    11041 taaccgctcc ggataactca gaaccacaac aggtttaatc cccgtgccgg aatttttccg
    11101 gcatcttttt cacaaagaat gccctcacgg gatattctgg tttaaaagag gattgaaaat
    11161 gtccgcagaa aaattcagag ttgtcatata caccaataca tgtgaaaaga aagtcagtta
    11221 tattcctgca acagaagata taaacatcgc tataatgcca tttcagacgg cagacaatgg
    11281 cttaattatt gtgtgtcaga actcccgtat aatacgacgc aaagagcgta ttcccggaag
    11341 cagctgtgaa tggaaagatg tgacattagc accaaaatcc agaaaccgcg ttcagttata
    11401 gcctgacagc ctgcttttca tcccccataa aagggggtaa aaattaaata tttcacaacc
    11461 gcgatatttt tcaggaatgc agattatttt ctgttatgac caattgagaa acaggcatga
    11521 gaacaatcac aacacgtgaa caattattaa ttaatgggag agtccacgaa agaatcgcca
    11581 cacatatagt tacaggagct catgggtatg aaacgctttg cacaaatggc tataacgtca
    11641 gatacaacaa ggcgcagcaa ctggttgaaa gctgcgaaaa aatagctgaa ggtaaactac
    11701 ctgttacctg tcctgtctgt ttcaccatat ggcaggatgt acacagattc acccacgttg
    11761 actttgacac acaatcgggg aaaagtgatt ttattgatac ctgccattcg gagatcataa
    11821 ccggacatgt ttcaataaca ggcaagaaat aaaataatgt tccagttctg aaaaacaata
    11881 tcacttgttt tctggaaact atcatttgca gcaggacgtt tttcttttta tatttccgga
    11941 accggaatgt tttagttttc ataatcaccc cgaacatgaa tgttcatcat caatatcctg
    12001 atgaaattat atttatctga agacctgtta ccagccacct gtcttatacc gtaacccgat
    12061 acataccgga tttattctat gaaaaagaaa tcacttacac tccttctgcc gtcattactc
    12121 agcggctgta cggcatttaa tgccacgcct gttcctgaca ccacgcctcc ggctctggtg
    12181 ttcgtcgacg gacaaattcg cgaaagcgct gccattatcg cccatacgca aaaacgtctg
    12241 cttccgccac cggtgacccg ccctgtgacg ccgccccgcc cgacaacaac agcgacgccc
    12301 acaaccgcag tcagcagcca gagtaccgca ctgcctcccc gtccgctcct tcaggccaca
    12361 cagctgcccg cactggttat gaccggtaaa cccggtcagt catccgtcgt ttatttacgt
    12421 gaggcacatc agcgctccgt cagtgactgg atacgccagt tgctaccacc cggctggcag
    12481 ttccggcaac agaacgctgc cataccggca ctgaacaaac gcctccccgt ctggtccgtt
    12541 aatgaccagt ggcaccgggc actgaatgcg ctgctggccg gccagcagct ttacggacat
    12601 atggactgga acaggaaaat actgactgtc acgacctctg caacaccgcc ggtcgacgtc
    12661 acggcaccgc agggttcaca gaaagctgct gatacaccgc gtaatccgtt caggggcagc
    12721 accgcaacgc cggcaggccc gacaccagtg acacctgccg tcagcgtttc ccgggctcag
    12781 accaccaccg ggacatccgg aactccagag agcagaacaa ccacgccagc cccggtggcc
    12841 acgaaaccgg tagtgccacc acagcagaag gtcataccgc cacggcagtg gaaaatggaa
    12901 aaggggacca ccctgaaaga cggactggcc cgctggacat catccgaaaa atgcccgcac
    12961 ggcacatgga cactgcgctg ggaaacacct gtcaactaca gcatcgatgc ccctctggtt
    13021 ttcaccggca gtttccgcca ggcgctggag cagctctttg cactctatac cccggctgaa
    13081 aaaccgctgt atgcccggac cagcactgca caatgcctgc tccgtgtgac agaccgcccg
    13141 caggaataac ctatgggatg ggttacgggt ttcattatgg tcattatggc cattggtaat
    13201 ttctggttcc acagtcacct cagccgtact gtgcaccatc agcagaccgc tgaaatcaca
    13261 cagcaggctg cagactttat tcgttatatg aatgccatca acgattacct gtatcagcac
    13321 cctgaacgac gtgctgccgg tggtcagctc acgtcagcac agctcggtct gcccgccacg
    13381 aaaaacgtca gccacctcat cagtcagcag cgcgtttttg tctgggcaaa agaaaaaccc
    13441 ggccttatgg gtgccctctt ggaacaaagt ggtgattcgg ccctgcttgc cagagtggaa
    13501 aacggtcgcc tcctggacac ccatgggcgg cgtatttcca tcacgctccc tgctgtgatc
    13561 cccgatcagg tcatcatctg gatgaattag gccggactct gaataactca cggatttatc
    13621 tatgaacaaa aaacttcttg ctattatgat tgcagccact ctgctgcagg gctgtaccag
    13681 tttccgacag gcagagaaca ccagaaaaga cgccgttgct gaacaacaac gggcccggga
    13741 aatgatcgct gatctgcgaa accggacacc ggttgtaaag gaatccccac gtcagtggat
    13801 caatccccgt ccggtcacgg ataaaaaccc cggtaatgcg gtgcccggct gtccgattat
    13861 cattaacacc cggcagagca tcacccttca gcaggtggcc cagcggatct cggaaaactg
    13921 tcatattcct gtaaggatca cccctgatgt acaggcgtat ctgagcgtaa ccacctccgg
    13981 cagtacgcag caaatccagg gcacagtacc accgccggat gccggcggta tggttccact
    14041 ggccgccata ggcagttctg acacgacact tccccgcctg cagggaaata cggcgtcact
    14101 tcctgaactg aaggcttcag gactgacatc actgctgaat gcggtcgctt cccgtctcgg
    14161 gatttcctgg cgttatgatg gttcacatat cacgttctat tatcttgata cccggacctt
    14221 cccggtgacc tatatggaca gccaggttgc ctataatgcc accgtggtca gcggtaccat
    14281 gtcatcaaat ggctcatcag gtggaacagc atcggatgcc atttccggtg atgccagtaa
    14341 tacccagacc accaccgtgg atatgaaatc tgcgctgtat caggatgtga aaaatgctgt
    14401 ctcatcaatg ctgacgccgg ggattggtcg catgttcatg tcgacgggct ttatcaccgt
    14461 tacggacacc ccacaggtac tggaaacggt ccggacgttc attgaaaaac gcaatgaaga
    14521 aatgaagcgc caggtggtgc tgaatgtgga aattctgagt atccggaaaa cccgtaatga
    14581 gcaggcaggt atcgactgga acgcagtctt cagcgacaga acgctgggac tgagcctcgg
    14641 cagcacattc accagtgcag ccagcgatac cgtcactggt ggtgtttcca ttgtgaacgg
    14701 gaaactcacc ggttcaaaag ccttcctgaa agcactctct tcccagggag atgtcagtgt
    14761 ggtaacccgg aattccgccg tgaccaaaaa cctgaccccg gtaccgatgc agattgccaa
    14821 tcagcagagc tacattgaga gtgtgacgac ggataccacc gccaatgtcg gctcatccac
    14881 ctccctgaat gcggccacca tcaccaccgg ttttaatatg acccttctgc cgttcattct
    14941 cccggactca cagacactgc agctgctcta ttccatgagt ctctccgaca aaccggttat
    15001 tgagaattac gagagtggtg gaagcaaagc ccagctgccg aatgtggatc tgaaaaccat
    15061 caaccagaca gtggacctga aatccgggca gacagtcatt atcagtggct tccagcagtc
    15121 agggcgtcgc agtggaaaac agggtgtcgg cacaccggga ttcttcggtc ttggcggtgg
    15181 gatcaacagt gaaaacgacg acaccattct cgtggtgctg atcacgccca atatcatctg
    15241 atttgcagaa ggaggactat cgtgacggat atttcaccga cagagcgcca ggtgtttccg
    15301 ctgacggcag gacggaatgt atttcttgcc ggaatggact ggaaaacact gcccgcaagc
    15361 cataaaaacc cccggacgtt tgcccgcagt ctgggggcag tacgctatat ttcctgtgag
    15421 tatctcagta cggaagatac cgatcgtcac atcatggttg cagccgtcag ccagaataca
    15481 ctccccaaag gcagccgcag gtatttctct ctcgccatgc ttatcctgcc cctgctcgag
    15541 tcaggaggat acgctatcgt tgaactgtca caggcaaacg atacagaact ttatggtttt
    15601 gtttctgctg ttgacggcat cctggtcagt gacctggtgg gaacccggga ggaaatcagg
    15661 gaggcccggg aaatattcct caccattaac agcgcgcctg aacacggctg gacatgttat
    15721 gaaccgccct cgtttaacgg gcctgacggc agaggtcccc tgccactgga aaccctgaca
    15781 ggtgcaggga aatatccggc agaggcacgc cttcaccccg tatcccgtgg gccacagctt
    15841 attccggtat tactcatact cacccttctg ggtactgcat ggtatggctg gcagtactat
    15901 gaacgcctga aggcagaaaa agcagcactt gctgaagcag cacaaaaagc cgcagaggaa
    15961 cggatcacac caccgtggct gagtatgccg gaaaccggag attttatccg gggatgcagt
    16021 gacgtatggt cacgcctgcc ggtgtcagtc gccggctggc gatattcact cgccgagtgt
    16081 gctgccaccg ggaacagcgg caatctgcgt gcatcatata caaacatcgc cgggacgaca
    16141 gtgaccgcat tcagccagcg ggtcagggaa attttcagca tccggccatt tatggttatg
    16201 ccggacggga atagcggtgg ttttgccctg ccggtcacct tcagtatgcc tgaaactccc
    16261 gttgccgttg aggcattacc ggaaaacacc ctcctgcagg aacggctgac cacactcgcc
    16321 cggtcgatgc agctgaaaat ggactggcag gaagtcagca acagcttcac cgatgaagac
    16381 ggaaatacca tccagccccc ctggaaggag tatgacctgc agatactgac gacacttccg
    16441 gcgcaccagg tggcagagca tttcagtgaa ccctccgtcc gttttatctc cgtaacaagg
    16501 caactggaag agggtcgttt ccgttatcaa ttcacaggta aatattatgt ccagtaaaac
    16561 aaaagctctt ctgctttccg tcctgatcct gccgacaacg gtcgcggccg gcaccatcag
    16621 caacatgcaa cagaataacc ggcaactcca ggtggaacag attcaggaag aaactgcact
    16681 gtatgaagcc atgctggcaa gagaaaaggt gcgacatgaa ctcagaaccc tggagcaggg
    16741 agaaccagct gagcctgtgc attcacagga aagcagttca gccgtcacga cagatacccc
    16801 tgcccccatt accggtattc cccggctgac agagatatcc ggagttcagc cccagataac
    16861 cgccaggata caactgccgg gcggacgaac tgctgatgta aaagaagggc aacacattcc
    16921 cggcaccaca ttccgcgtgg aaaagatcac cactgacagt gttgtccttg aacaaaatgg
    16981 tatcagaaca acactacaac cctgactcag tgccgccatt tacaggcggc tgtcggaaca
    17041 gattattccc ccggccctta atcacaaaaa aatcgtttcc gggtaccgct gcccgtaagg
    17101 agattttatg ctggcacctg cagaccagcc acctgatgac attctggatt acgtggttgt
    17161 tgatacagat aaaacaggac aaattcgtgt cagagtggtg gaaggaaaaa aacaccttcg
    17221 tgccgtccag gattatctga cccgcctgtg ctcccgctat ccgggtaaag tatccccgtt
    17281 tgagttcacc acacttgatg tgatcgcccg actcaggcag gagacggtca tttccggggt
    17341 ggaaaacgga attaaccccg tacagcaaaa aatgctcggg tatatcagcc atgcggccac
    17401 cctcagcgcc tcagatcttc acatcacgcc ggggcgtgac agtacggatt tcacgtatct
    17461 ggaggcccgt gtccacggag agctggagct gctcgatatc gtcagaaaag atgaggggct
    17521 ggaattactg ggggccacct attccggaat gactgacgtc ataaagggaa cacagtttga
    17581 tcccggtgtc ccccaggatg cccgtctggc tgaaagattc ctgaaactgg ccggactgtt
    17641 tggtgcgcgc tactcccact atccgtgtgt gggcggactc tatgccgtac tgcgtctgat
    17701 caaggatgac agtcagcata taccgacatt cagtatgctg gggtatcacc ctgagcagga
    17761 gcgcgctgtc cgaaggatgc tgcagcgacc ggagggcatc gtgatcctct ctggccccac
    17821 cggttccgga aaatccacca cgctgaggac ggcttctgcc gcctatctgg aacagtatgg
    17881 cttcaacgat accggaggga tcctgctccc ccggcgacgt ctgttcacaa tagaatcacc
    17941 accagagggg cgtatccccg gtgcaattca gacggccgtt atggataccg ctcagggctg
    18001 ggtcgattct gttaagtccg ccctgcgcct tgatcctgac gctatcctga acggggagat
    18061 acgtgaccat gcctccgcca tcacggcgat aaaagccgcc atgaccggtc acctgatgct
    18121 gaccaccctt catgctaacg acccgataaa tattcttgag cgactggaaa tggagggcgt
    18181 tcaggcaagg atgattgcag atccgcagtt atttatcggc ctgctcagcc agcgtctggt
    18241 tcaggtgata tgtccccact gccgcctccc ctggcatgag gtggagagct cccggacgga
    18301 tgaagaacga cgcctggtgg aaaacttctg ccagcccgat gccgtgtatc ttcgtaacca
    18361 taacggctgc ccacactgct ggcgtggagt gaacggccgg accgtcattg cagaagtgat
    18421 ttccccggat gcgaagtttt tccagattta ccgggaaaaa ggccggattg aggcaaaaac
    18481 ctactggcac cgggagctcg gcggtatgac acgaaatcag cacctgctgg gcaaaatcaa
    18541 cagtggtcag gtcgatccgc tggctgcgca ttacatatcc cctgtagacg aggacagcta
    18601 tacgctgctg cactgatatg aaaattctgg aaagactcaa caatacggaa ctggaactgt
    18661 ccggccttaa ccgctggctg ggcaaaaaga cgttcaggcg gactacgttt tacgagtccc
    18721 ttgcaggcat gatccgggac ggcacaccgg ttatgcgggc actggagttc atctgcgatg
    18781 tggaaacgga ttttggtaaa aagaaagggc agtccggcct gtattttctg gcgacagact
    18841 gtatcgccag catacgcagc agtggtcagc tctctccggc cctgaaggac tgggtaccca
    18901 aagatgaaat tgccctgatc cgtaacggtg aagagcgcgg tgatatcgct gaagccatgt
    18961 ttcaggttgt caaaacggca aaaggacgac aggagatgat tagcagtctg gtgagtgtct
    19021 gcctctatcc gctcatcctg ctgacgctct gtgtggtgaa tatgtataac gttcataccc
    19081 gggttattcc gatgatcagc gcctttgcac cggaagaacg ctggtcagtg cagatggtca
    19141 ttctggccaa atcctccgag ctggttatcg gttatggttt gtggctgctg gctggactgc
    19201 ttctgctggc aattctcatc aggatgtcat tcagtctgta taaaggtccc cgacgacggt
    19261 atcttgatcg catcccgccg tggtccctgt atcagacatt ccatggcgtg aattaccttt
    19321 ttaatatttc atccatgctg cagataaata ttcctttatc tcatgccctt accagaatag
    19381 aaaaacatgc agaacataac cgatggttaa aatcacgaat aagtgctatc agaaaaaatg
    19441 tcttatccgg tcagtcactg gcactggcca tgaaaaacag tggttatgac tttccgtcaa
    19501 aatcctgtgt gaataatctt cttttaaatt cagaaaggga ggacagtgtt gccagtatgg
    19561 cgctttatgc agaccgctgg ctggaagaag caaaaaagag tgtcagaaaa agcggcatta
    19621 ttattactgc tatatcaggt ctgctggtat ttctcttcat catcaatatg gtcagtgcca
    19681 tttacggcat cagtgacatg ctgaagcagt aaacacagca tcctttatat tcccctcgat
    19741 ccaaattctg ttttaccaat ctcaggagct aatatgagcg tcataacctg ttcgtattca
    19801 gaacataata ttcgtcacct tcagaacgat gataaaggca tgaccctgct ggaagtcctc
    19861 ggtgttctgg tcgtcgctgc cattgttatt ggtgccgtga tgggacttat gtccgatacc
    19921 ctgtcctctt cagacaacca gaaggaactc aaaaacctgc agacaatcgc caccaaaatg
    19981 aaagcccaga aattccaggg gcagtataca ggtactgact acgtcaaaat cctgaccgaa
    20041 agtggtggcc tgcctgccga tatgatcgcc ggtggtaata aggcgaaaaa tgcctggggt
    20101 ggtgcggtaa ccatcaaagt cagttcagat aaatactcct acgttattga atccagcaat
    20161 gtgccgaaga aaaactgtat tgatctggtc accagcctgc gcagttcatc catgtttaca
    20221 aaaatcaacg gcaatgtgac gaataaagtt gacccgtcta cagtatgtaa cgcagataaa
    20281 actactatca aactggaaac caacagttaa tcattcatca gcgcccgccc gggcgcttta
    20341 tttaaggtca ctgtatgaac aggttattta tcctgacagt gattttttca ctgttatgct
    20401 cacttccttc cgcacaggca tccccacagg acaaacaata tgatcgttgt tttattaccg
    20461 ccggaaaacg ttattccatt gatccaggat tactcaaaat tattgcccgc agggaaagca
    20521 gtctgaatcc ccgggccatt aatcataaca ggaatgacag aggaaaaatt atcagtaccg
    20581 attacgggat aatgcagatt aactccacac atattcccgg gctgaaaaaa atgggcatta
    20641 tccggagtga gaaagatctg ttgaataatg tctgcctgaa tattcagacc ggagcatgga
    20701 tcctcgcccg gcattttcag cgatgcggcg taaactggga atgcctcggc agttataacg
    20761 ccggcttcag taaaagtaac actcaccgcc ggatgaaata cgcccggcag atttacagcg
    20821 cttacatgca gggacgttag tccgggagaa ctgccatgct ccagatgatc ccggcactgc
    20881 tggtcgccat aaccgtggta atacagtgtc tggccgttca gatgagtcgc tatcgactct
    20941 cccggatgaa tgcccgcccc cctcaccatg gactggcgct gatgagcggg atgcttgcag
    21001 ctgccgccat tatcctgcgt ctttatcaga atgacagcgc tgtcggggtg ctgtttatga
    21061 cgataatgac cggatgtatg acactgatga ccctgacgga tgctgcatcc tgccgtcttc
    21121 cccggccctt caccgtcatg tatctgataa gcggcagcgc atttcgtctc tggcagggag
    21181 actggctgac aggcctgtgc agtgcagggt tctggttcct gctgttgctt ctgttcaggc
    21241 aatacacctc ttaccgcagg aaaacagaaa ccataggcct gggcgatgtt tttctgattg
    21301 ccggtattgc catctggtcc ccgccgggag acatcccctg gattgtcgcg acggcagcag
    21361 gcggagcctt actttatctg cggtgtaaca gacaacaacg cattaccaga gaacttccct
    21421 ttgggccgtt tctctgcgca tcacagtacg catttacttt ttatcccggt ggcctttggt
    21481 aataccggag ggaacgacta tgcaaaaaga caacgataaa ggttttgcac ttcttgaaat
    21541 tctgggagga ctggttgtca tcagcctgct catgccgctg ttctggtctt acattgaaga
    21601 ctacctgaat gaaatgcgca accagagcgc cgcatttcat gcagatgcct acaatactgc
    21661 agccaggacc tacatcgccg ataacaatgc ccgcctgcat agcggaacgc ttcctgccac
    21721 cttcacggcg gatgagttaa tccggaaagg atatctgaag ggtcttaacc gctcgccctt
    21781 tgggcagagt tataccaccg gcatccgtcg taacacgagt accggtcgtc tggaggccct
    21841 gacctgctcc accggagggg agaatatcaa agacgatgcc ctgcgcagta tcgccagcct
    21901 gctccccggt ctgggcggat ttataggaaa aaatggcaca gcaaccggtg tttttggtgg
    21961 atggacagat aaacccggcg actatggact cagctgcaat ggcgggcaca tcgccatcgt
    22021 aatgatgggt gatgacctgc aggaaagcga tcgcctgtat cgctttcagg taccgggacg
    22081 accggagctg aaccagatga acacggccat caatatggga gggaacaacc tgaacaatgc
    22141 aggaaacgtc aatggacaaa gtgcaacact gaaaggtgat gttacaagcg aaaacggctg
    22201 gttaatcacc aaaaatgata aaggctggaa gaacatcacc tatggtggcg gttttacgat
    22261 gacagacagc cagtggatac gcgcagtcgg tggtaaagga attataacca caggggaaat
    22321 caaaggagga aaagtcagtg gggggacagt ccggtctgac ggtcgccttt ccacaggtga
    22381 gtatttacag ctggataaaa cagccgtagc aaatacaaag tgttcaccag atggacttgt
    22441 aggccgcgac agcaaagggg caatactttc gtgccaatcc ggtgtgtggg ttggctttga
    22501 atcctaccct gtgggaagcc ctatcccgtg gccatctgcc actcctccac aaggctatct
    22561 gttgatgaac ggccagtcgt tttcctgttc ccggtatcct cagcttgccc gtgcttatcc
    22621 tagctgtaag ttacccgatc tgcgcggtgt ttttatccgt ggctgggata acgggaaggg
    22681 acttgatggt gatcggaaca gacagctcct gagttatcag gccgatcaat caggcgtcta
    22741 tcacgaacgt ggcggatggc tgaaagggca tcatagtggt atgccatact gggcacaggg
    22801 gagtaccaca gaaatgcgtc ccaaaaacat tgcattcaac tacattgtta aagccagcta
    22861 gggactcaca tctgataggt atacacagag aaaacaccgt taccacaggc gtaattttgt
    22921 gttggtctgg agatgatttg atatgacgct cccactggca cggcaaatga aataaatctc
    22981 cgggcatatc agctggtcag caaactcgat gccaggcgta aacagaccag caaaatttcc
    23041 ccttattttg ttccctaccc tgcaactgtc gggtgcagaa acgggctgtt tgtcgtgaca
    23101 ctgcacgatt tcgaccttga aacccgtgca gaaacccggg gactggcaat atctcatgcc
    23161 agcatgctca gtcacctcct gtccgaatcc ttttcattcg cccttaaaac agagttgcaa
    23221 acactcttga aacacactcg atcataacga tcgaaataac tcgcttccgt ttaaaaaaca
    23281 ctctctttag actcaattta tgaacaaaac acgctgaccc gcatggaggt gaaatgaaaa
    23341 ctgacagcac aggtattgcc gcccgtatga tgctctccct ggacagggag cgaatttgcg
    23401 aatgcctgct cagtcatcgc cagctgcagt ccacacccct gcaggttcgt tatccgcagg
    23461 gagtcaggga tgcccttggt atcatgagcg aacagctttc tctttccgtc tctgacctga
    23521 cgcgtatcct ggtcgaagac gcgttaagtg aaatgtttct tcctgcagat aacatcgtcc
    23581 gccgtcttct cagcaggatg gagcacatca tgcaggcaca tgatatcagc gcaacaacga
    23641 tggctgcatt actggccccc tggaatatcc gccctgccgt cttccgggag ccggacagac
    23701 tcactgacta tctgaccggg gagattcttg cagcactggc tgactggttt tatctcagtc
    23761 cggaatggct gaacggcagg gtgcattatc cactgtatcg tccgggagac tggccagcca
    23821 cacaggagat attctgcagg attatttcag cccgcgagaa tatggacatt attctctggc
    23881 atggttttcc gtttgccgga acgcattccg gagaatattg cggcgttctg ctcaggcaga
    23941 aaaaagaaat taataacacc attatttatc cggtactttc gttatatccg gccagaatgg
    24001 atatagaaaa agaaggctgg tttcagatgg cccgaaaaat atctccggat atccctgttc
    24061 gcgccgtaac actcactccg gcacaggcag aatatctgat aacaggaaaa atattaccca
    24121 ctgcgttatt cagggttccg ttatccccct ggtaataata ctgagcaata aaccggaata
    24181 atacaccaac tgtttttaat ggcacagccg tgccctctgt tgttataccc aaaatacctc
    24241 aatacaggaa actgaaatga tgatgaaaaa gcatattacc cgtactctca tcgcgtctgc
    24301 cgttttattt tcctttaatt cgactgcagc aacgtcatat tttgaagccc gcaatgatgc
    24361 gatgggagga acgggggttg cctcctctca ctatggtgtg gccccactgg caaacccggc
    24421 actgctgaca aaatccggtg caaaagatga cttcagtctt ctgcttccgt ccgtgggtgc
    24481 gcagctgtcc gatccgggta atatcgccga caacgccgac aaaatcagcg atgactggaa
    24541 agccttcgac cgtgccgtcg acagccactc cggggtaccg cagacagcgg cccgcctgaa
    24601 agagagactg caggacttcc ggaacaccca tgccagcgca caggccgggg tatctgctgt
    24661 ggcggccctt cccggtgata ccctcgctgc tgcactgatg ctgaaaacgc tcgggactgt
    24721 cagcgtcgac ggtaaagtca gcgatgccga cctgaactat ctggaaagca ttgcagacag
    24781 tggcagccag gatgtggata aaaacagact gacctcacag gcttttgccc gtgccgcact
    24841 gatcaccgat gtgggcgttg cgctggccac tgaactggaa acggccggac aaaaatggtc
    24901 actgggcttc acaccaaaat tccagcgtgt tgacctgttt aactacaata cgctgatcaa
    24961 aaactatgac agcagcgcat tcaaaggtga ccgttaccac aacacccaaa acggtattaa
    25021 cgctgatatc ggtgccagca tggacctgga tgacaactgg acgctgggtc tggtcgcaca
    25081 gaacctgatc ccacgcagca ttgagaccaa agaggtacat ggtgtcactg aaaccttcag
    25141 gatccgtccg caagtaacaa ccggcgtatc atggcacaac gacatgttca ccacggcact
    25201 ggatgtggat ctgaccccgg ccagcggctt cacatcagac agcaaacgtc agtttgccgc
    25261 cgtaggtgct gagtttaatg cctggaaatg ggcacagtta cgtaccggtt accgccagaa
    25321 tctggccagc aacaacggca gtgcattcac tgccgggatt gggctctcac cgtttgatgt
    25381 gattcacatt gatgtcgccg gcctcgtggg caccgacaac aactacggtg ccattgctca
    25441 gttacagttc accttctgat tatcacgccc cgggacgccc ggggcttctg acctgaatct
    25501 gctcgtttcc gctcttctgg ccactcaccg ccagtcctgt tcattatttt ccggataaaa
    25561 ccatgcaaaa aaaacgtatc acccttgcag ggggagttgc catgctctgt ctgagcggct
    25621 gtcatcttcc cccggataat accgcaccgg ccaggaagta cggtgcagct tctgaagtcg
    25681 ctgtcacacg acatgcacag tatcagttgt gggagcaggg cacgtattct gcagccctgc
    25741 cttcccgcca gcccggaggc agcctgcagg ctcacagcgg gcgggtttcc tttgactggg
    25801 aaggcgatgc tctggagctc ctgagcgagc tggcccgtct gcgcggacaa cagttcagtt
    25861 acagcggcgt gcgtcttccc ctgcccgtct cactccatgt acgcgatatg accttcgctg
    25921 atgcccttcg tctcacagag gcccagactg cctggcgggc agtcctctca cagtcagccg
    25981 ggcgtttaca tctttcctat atgccaacgg agaaaaaatg aaaaaacagg tctgtctcct
    26041 gctgtccggg ctcctgctgg caggctgtgc cggacagcac catcgccccc ctgccggtat
    26101 ggcaggcaat ttaccaccgc cggatctccg ggcatggctg acgcccactg acaaccagga
    26161 tgacggcatt tcagacaccc gtcgccagat gctcacggac gccggacgaa cgctgggatt
    26221 ccgtggcgga aaagcacagc gctcatggga actgattcag gcactgaatg cccgggaaag
    26281 tacactgaat gccctgtatg acttccgtcc ccttatcagc agggaaggct ggctaccccc
    26341 tgttattgat gaagcgcagg atgttgctca cattcagcct gaccagataa gaaccgcgtc
    26401 ccgtgtctgg accatcctgc gccccgaacg ttttgtcagt aacccgccgg gctggcgtga
    26461 ctggcttctg cgggggcttt ccactaccgc caccccggga actgagggcc gtgttgtacc
    26521 ggaagaccgc gcacagcgca gactctggga aaacgcactc cgccagggct ggcaggaagg
    26581 ccgtgacaac gctgacctca ccctggaagc caaccagaaa cgcctgaccc gggattaccg
    26641 gggaatgatg ctttatgccc tgctctggcg tcagggcatg atcacacgcc cggatgtgac
    26701 tgaacagcgg cagaccgtca ccggtaacgg caggaaactc atcaccggag accacgtccg
    26761 ccgtctgaag acacacgcgg aattcaccct gcagaaatca cgctggcgtc cggtcgtcag
    26821 cacggaagga gcccccgatg aaattacccg atgagttcgg cacattccct ttcgcccgtt
    26881 tcacggccga agagtttcgc tatttttttg cctggtgtgc ccgacaccgc gtcagtgata
    26941 tcgatctgac aggcggctca ccggtatctg tcagtcgctt tggacggcgg gtacgttcgt
    27001 ccgcacatcc tctgccgtcc tccatcatga tggcgctgag tgatgaattg tttggacggg
    27061 aagtccttcc ccgggtgctg tccggtcatc ccgtggaccg taccattcag gtcagcggtg
    27121 atgcctccgg tcgctacggt ctgcaacggg ggcagcgtgt tcgcctgcgc tgtcatctga
    27181 ttcagggaac atcagcaaca gaagaaaaag taatctccgt caccatgcgg gtgatcccgt
    27241 ccgatattcc ggacatcctc accatgaata ttgaaccgga cctgctggat gccatgctcc
    27301 ggaaaaccgg tctgggtttc atctgcggag agaccggctc cggtaaatcc acactggcag
    27361 cggcactgta ccgctacatt cagacgcatt tccccgaccg caaaacggtg acctatgaag
    27421 atccggtgga atacattctg gggggagaga atgaccttct gccaccccat caggccgaaa
    27481 tcgggcgtga tgtggaaagt tttgcggcag gacttcgctc tgccgttcgc cgtaacccgg
    27541 acatcatcgg tatcggtgaa atcagggatt atgaaacggc agatgcagcc gtccaggcag
    27601 gtaacaccgg tcacttctgc atcggcacca tgcacaccaa atcacccggt gaaacctttg
    27661 cccggctgct ggggttattt ccaccggaaa tcagggattc aatggcagcc gccacccttt
    27721 ccctggtgca gttcattctg gtgcaggtcc tggtcagaac aaatgacggt ggcagacagg
    27781 ccgtccggga atacatcgtc ataactgacg aactgcgtga tacgctctcc cggcagtccc
    27841 atgcgacatg ggggcattac attgatgaaa tcattcgcaa ggaaaaacgg cggatacggg
    27901 atcaggttct gaccatgtat cagacgggca gcattgaggc atcagaagct gcgctcttca
    27961 tcccggccgg agaattcccc aaatgaaacg cgaaggcttc tggcgggatg tcactccgcc
    28021 actgaatatt tatggtgtcc cgttgccgtt taccatgatt tacctgctgc tgtttccgta
    28081 tccgtcaaaa acaacattct gggtgtgcag cggcattttg ctgttttttg tgctcctggg
    28141 gtactcagga tggactgtcc ggacgctctg ccggcgtctg tacagcctgg tgcagggtaa
    28201 acaggcgtca ggccgtccct ggtggtaccg gcatcgcaca gaattacccc gtgaatggcg
    28261 cggaacggaa taaccctctt ttgttcatca tcccggcgta tcacaacgcc ctcatcttta
    28321 atccccggag aacgacatga agtccagata tcgaacctgg cttgctgtcc cgcctgagga
    28381 gacagaagcc gtaaaaaatg cagttccccc gttaaatggc cgcaaagctg tcgcatggga
    28441 tccagaaaaa aagctctggt atgcccgggc gggtaccgaa ctttcactgc tggagcgctg
    28501 gttaccccgg ccacaggaac tgtcgatgga tgccggcgat ccggtcactg aatttgctca
    28561 ggtcctggaa aacgccgggc tggttattca gggattgcca cagatggacg gagccataca
    28621 cagagtggcc acccgggatg acaaaaaagg ggccaaaagt ggcgcataca gggcctatct
    28681 tgatggtcgt cccgccggct ggtaccgcga ctaccggagc gccgatgaca gtcccacaaa
    28741 ctgggtattt tccggcggag aacaacacga tcctctggcc agactgcatc tgcgggcatt
    28801 cgctcagcaa cagcgcgatg ataacgctcg taaactgcag caacagtata acaaacaggc
    28861 cggctatgcc cgctcctata ttaacaggtt gccacaggca acagcacatg aatacctgac
    28921 gcgcaagggc atccgggccg cgcctggcgt gcggctgaac aataaaaatg agctggttat
    28981 tcccttcagc aacggccggg gagaaatacg ttcgtaccag cgtattccgg tcaccggcgg
    29041 gaaggatgcc cgcattctga aagactccga gaaaaccggc aactggttca ccttcggaac
    29101 cccggaaaat ggccgccccc tgttatttgc ggaaggttac gctactgccg cctccctgca
    29161 cgaagcaacc ggccttccgg ttctgatgac agttgatgcc agcaatatga ttgccgtggc
    29221 agaaaatgcc cgccaaatct ggactgacag cccctttgtc ttctgtgccg acaacgatca
    29281 tcaacgggaa attaacaaag gggtttttag tgcaacaaag gccgcagaag tcacgaatgg
    29341 cgaagtcatt attccggcct ttacggaggc ggaaaaagca cagggactga ccgatttcaa
    29401 tgacctggac gccagccggg gacgggacaa ctttcagaat gccatgaatg cacagttaaa
    29461 acatatcggt atccccacac ccaacagtga cactgccgac catcgtgagg ctgtcgtgat
    29521 cgacaacctg atattcacac cggtaaaaga tgagaagcca caaatgtccc ctgaaaacag
    29581 acagagcaca gccccggaaa cggagctggc gacacaggat acgccttatg acacccactc
    29641 cgacgataca gaaaaccagg catatgaaga tgactatgcc gcctatgcat ccctgcaatc
    29701 agtaactgcg gagacgttat ccccggataa accactggag aatattgcgt ctgccgctga
    29761 taccggcaca gagaatacct ctgtgcctac cgctggcgac caggaagacc tcaatacaga
    29821 cgccacaggt acagacaccg ctcacaacag cgacacagca gaaacaggaa cgcttcctga
    29881 acctgccggg atgcagtccg tgcagcaggc aaccactgaa ccgaccgcag aagataaacc
    29941 acacgagaac gttgtttccg ctgccgatac cagagaagaa aatgcctctg tggctaccgc
    30001 tgataccggt gaagaaaatg cctctgccac caccgctgac aaccagagca cgcaggcaga
    30061 agaacatccc cggagtaaag acacaccgga aacctccctc tccgacgcgg ccgggaccca
    30121 gcacaccacc acaacgcagt ctgaagaaca agtgcaggtt tccatggcca gcgctgacga
    30181 ctggagcgca tttgcggaag atctcaacgc tgacactccg ggggccctca ctgttcacaa
    30241 caccgacaac gcggagacaa acacagtttc tgaaccggcc gggatgcggc cagttcagca
    30301 ggtgaccact gaactgacgg cagaagataa agcacaggaa aagagcgttg cgtctgccgc
    30361 tgagaccggc gcagagcact cctctgacat caccgatgac aacaggaacg cacccgctga
    30421 ggagctcaat gccgatgcca cggttgctgt tcacaacacc ggcacaacag aaacagcctc
    30481 accttctgaa cctgcagaaa ctcagccagt ccggcaggcc accgctgaac cgacggctga
    30541 agataaactg ccggaagagc gtcttgtttc cgccactgag accagcacag cgaatgaagc
    30601 agcccgccct gaaacgccat cgcttttcag ccgtctgcgc cgttttctga ccggtgtgaa
    30661 atcacgcacc tcagaacctt ccgttattca ggagaaaaag cccacggagg ctgaaacagc
    30721 aacagaggag gtcgcaccac catcaccacc ggaaaccagc ccggaagaca catcgtcagc
    30781 ggaaccggac acgattatct ttgcaccccg ccgtcccgac agtccgcagc ggcacaacct
    30841 ggacgagatc cttaaaaatc ttacacacaa agagtttccc gacaggactg cgctctacag
    30901 cgttgacggt gaacccgcct tcctggacag actctactgt ctcgaaatgg tggatggcgc
    30961 cagcgcggat gacaaaaagg tcctggctgc ccttgctgtg gccaccaact tctatggcgg
    31021 cgttatcgag ctgaccggca gtgatgcctt taagcaaaaa gccatgcagc tgattgtcga
    31081 atacgacatc aaggttcgca tgaaactccc ggcccagcgg gcagcactgg aaaaaatccg
    31141 caaggaaaaa gagatggcgc atgatgccat tgtcacccac agacccacgc cggaactcaa
    31201 ccgccagtca ccggaagaga caacaatggc ccaaccggct ccccctgtgt caccgaaggc
    31261 agaggctgaa caaacagcag gcagtacggc tgaaacaccg catccggaag cgccggtcac
    31321 ggcaaaagct gaagcccccc gggccgacag accggaacaa cccgccgccc cgtcgtctgc
    31381 gccagaaaaa gcccctgtca gtgcacagcc gcagcaaccc gacacaccgg aggaagagcc
    31441 gcccggcaag ctcagccccg gtgaaagtgt gactgccgtc ctgacaaact acggagaaaa
    31501 agagtatgcg cccggaaaag ggcgctgctt ttttgtggaa ctcaccaacc gcagtggcaa
    31561 acgggaatac tggggaaacg gcctggaaga actggtcaaa aaccatcaga agggtgaccc
    31621 cgtcacactg acactgcagg cgagagaaac attccggctg tccggtgaaa cgcgggatcg
    31681 tatccgtaat atctgggcca taaccccggt cagtaacggc attaccgtta ataatgacca
    31741 acctcatgcg ggtcagcata ccctggagtt tccgatggca acattcagcc aggtgatgtt
    31801 acagatgcaa caacactggc cacagataat gcaggaagtt cgctccccca aaaatcctcc
    31861 caataatctg tacattggtg aagacaggca acctgcatca gcacctcagg atcaatcaat
    31921 gcgactcccc ctcgctggta atgcccccaa tgaactgata ccagccatcg cctctgccga
    31981 taaggacaat cgtcagttga atcttctgct ggtccacagc gcagatgatc atctgcaggg
    32041 cgtggtccgc ctgaacggca ccctttaccc ggcactggcc accccgtcag ctgacaaccg
    32101 tcagctggtt atcaatgccc tgacggataa cggtctgcag tttgccggtt acggtgaagc
    32161 tgttaaccat gatgagaata atcatcagcg tccgtcccca caaataatgc agtttcacct
    32221 gaagcaacag gactccccgt tgtttgccgc catccacaaa ccggaagagc aaccggacaa
    32281 gcttttccgc tctctgggtt ttgagcagac ctggaaagag tggtctgaca gccaaaaggc
    32341 ggaggacagg caggaaaaaa ctctgcaaca ggcacaatcc cacagccccg gccgatgagc
    32401 cggtaaggac gaccatgaac atgaaaaaaa cgctgctggt catactgacc ggcatcaccc
    32461 tggcatcacc ggtggcaggt gccgccacct gccgggcggg ggccgctgcc gaagccggca
    32521 gcagagccgc ctgggagcgg gcgcgcacag ccagccaggc atggtcacag cgggagaatg
    32581 acgtcgcctc ctccctgcag tcctgcctgt cccgcatcaa ggacatcagt atcactcttc
    32641 cgcagttccc cagcctggac aagctcctcc gcgacctgga aaaccaggtc tgtgatgccg
    32701 tcgtggatcg ggtgaatgac catcttccgg ggaatatcga cccctggaaa gactacaacc
    32761 tctgaacagg agcaccatga gcgaagcaca acccacctcc cctgtctcac cggaagcgga
    32821 taacagcgga gatatttata accccgtgct cagaaccatc gcacagtcag aacaacgggc
    32881 cgcgctggta cccggtctgg tcagggcgtt gctgtacagc ggcgcagcac tctgcatcag
    32941 cctggtggct aatggcatac agtactggca tgccaccggc gtgacgcgcg aatactttgc
    33001 cactgacaat ggccgactgg tccgtctggc accaaccagc cagccggcct ggagtcagag
    33061 cgacgtcatg aactttggca gcatgacgct gagcgaagcc ttcaatctcg attttgtcca
    33121 ctaccgaaaa caaatcacga cacaggcccc ccgcttttcc gaagccggct ttgccggcta
    33181 tgtgaatgcc cttcaggcct cgaacattct cgacactatt aaaaaggaga aatttaacct
    33241 cacaaccact atcggggcag gtaccctggt caaccaggga cagatggaga acggcgtctg
    33301 gttctggaaa ttccagtatc cggtcaggat gcgcctggtc ggtcagacca gcacccgccc
    33361 cgaacaggcc ttcacctttg aaatcactgt ccggcgcgtt gacccccgca tcaaacccgg
    33421 cggcatggaa atcagccaga tggtttcccg taacgccgca gcaggaatct gagactcata
    33481 tcatgcacat acatcatctt attggcgcac tgcttattgt gtgcggcact gtcacagctg
    33541 cagataaccc gggctggaaa aatgcacgaa gcccgcagac cggcggaaag accgcacagg
    33601 caaccgggaa caccccggca ggcggtcccc cgccagccgc acaactcccg ggaaacctgc
    33661 cgcctcccgg cgctgctctt gtacaggaag gcgcatcgct ggattctccc ctgaatgcca
    33721 gtgaaattcg tgaactccgt gggataatgg ccgataacga acgggccatc agtgccccgc
    33781 tcaccagtgt ggtgccccgt atcagttccc tcacggtgaa tctgtctccg ggggccagcc
    33841 tgccccttgt ccgtaccgct ctgcataacg tcagcgtggt gaccttcacg gacgtcaacg
    33901 gcagtccgtg gccacagtcg gatccgccct ataacgctgc gcccggtcac tttgacgtgc
    33961 agttcaacgg caatatggtg tccgtcaccc ccctgcgcgc ctgggctgcc gggaatgtga
    34021 cggtctatct caaaggattg tctgtaccgg tcatcctgaa tatcaccagt ggtgaaacgg
    34081 acaccactac cctgagtcag gaaatcgaca gtcgtctgga tctgcgcatt ccccggcggg
    34141 gacctgccag cccggtggtg tccataccgt cagaaaaaat tgccctgcat gacgccactc
    34201 tgcaggcctt tctggacggt gtgccaccgg atgatcccgc catccgccgc ctgaaattct
    34261 ccggtaacgt cccggacacc accatctggc aacacggcga tgatcttctc gtgcgctcac
    34321 gggccatgct gcgggatgaa tttgaacaga cgctggcatc ggctgatggt acgcggctgt
    34381 ggaaactgcc ggtgaccccc ttactcacct tctccgtgaa tggtcagtcc gttcacgtca
    34441 ccccggaact ggagtaaatc atgagcgcag aacaagaaca ggacacagcc gcaaaatccg
    34501 gcaaaaagct gtcagccctg ctggttatgg ggggcgtgat tatcatcggg ggcggatacc
    34561 tgctgctcac ctggctgtcc cccgaaaacg ctgcatcgct ctcgtcagtc aatatcaaca
    34621 gcacaacgtc cggcggcgga cggaacgtga tggagacaca gcactaccgg gatttactgc
    34681 gggctgacaa tgaacgtggt gctgcagaag cgttacgtaa taacgagacg tttatcgcca
    34741 gtctgccccg gggactggat accgataccg gaaacgatca gaaaagggat aatccccccg
    34801 atgatcagtc aggcacttcc gggcaaaacg catccctccc tcaggaagat cagactgccc
    34861 gggaaaaacg gcgtgaggca ttacagaaac tgcttgcccg tatcaataca cagcatcagg
    34921 atgcccggcc accggtagtg gcagcagcca tgtggagcac caccggcact cccgggacag
    34981 ccccacatgc gtcaccgtcc ggtaccgtta catcacagca tccccgtctg caggagacga
    35041 gtctggcaac accggctaca cgtcagggga tccagctgat accggcactg acccgcgtac
    35101 cggcttacat cgacaccgaa gtggactctg acaatgcggt ctcccgtgtg gtggcctccg
    35161 tacctgccgg cccatgggcc ggggccgtcc tctacagcca ggacgccaga ctcgcaggtc
    35221 agggaatgga tatccacttt gaccggatga tgtggcgggg catgtttctg aaggtgaatg
    35281 catatgccct gaatgaacga acaggaatgt caaccgtggc cagcgacgtg aaccaccgct
    35341 ggtttaagca catcgttctg ccgtccgtac ttggcggggt gggcaatgtc ggaagtcttt
    35401 acaaagacgc caacacacag atccttcagg gaaactacgg cagtattacc ggcagggtgg
    35461 gaatgcctga tggagaggtg ctggccggcg tcattgcagg tggcatggcc gaacggggca
    35521 gccagctgct gacccgccag gcagagagcg aaccttaccg tcaggtgaca gtatcacggg
    35581 gcgaggtcgt ctccattctg tttgttgagc cggtcatgag caacgatatc agtacgccgt
    35641 cctccggcac tgccccttct gagtccccct cctccggacg tccgctcacc caggcggagg
    35701 cagagcacca ggcctctgac aggatccggg cggcggtcct ccgtaaacag aatgaaatgc
    35761 agcaacgcta cggcatacaa caggaaagtt atccatgaaa aacgaacatg aagatcccgg
    35821 cagctttggc gaaacgacgg caccggcatc cggcaaatcc ttcctgaaac gccagtatat
    35881 gggatacagc ctgccggtct gggccggctg tgccctcatc atcctggtcg ccatctggta
    35941 caagtttctg cggacaccct cagagccccc tgcagatgca ggctttgctg tcacaccgcc
    36001 cgctgctgag agctgggcag agcccatgac catgacggag tccgggatcg gaaacaacat
    36061 gcccgcaccg ccggttaccc cacaggcaga catgcagcaa cttgcccgtg atctgaaagc
    36121 agagctggac agacgggatg aggaaatccg gcgcaccctg accatgctga aggacagtat
    36181 caccaccctc agtaacgcca ttcagaagga tgaggcctgg gcacaggaaa cccgtcagtt
    36241 gcttgaggag acccgtaagc aggtgcatac cctgcagatg cagggagcac tgccagcccc
    36301 gaccgggaat aacagacagc ctgcgaaaac cctgccttca cctctgcagg gcatgaaaat
    36361 catgtcggtg gagaaaggca tggcctggat ccgctggcag ggcagtgcct gggcagtacg
    36421 ggaaggcgac agtctgggaa acatcaccat cacccggatt gacccgtcca gccgggcagt
    36481 catcacctct gccggtacat tacgatgatg agcattgatg ccctgacagc catagagaac
    36541 tttgccagcg gtattttcag tgcaggcatg gagtttctct ttacctgggg agagctgctg
    36601 gggattatcg gccttatcgg tcatctgatg cgcgcccggg cagaaggacg tcacagtatg
    36661 ggaccgggaa aatttattgc cggtatcttc atctgcggca tgctggtcag tctgccctca
    36721 atgatcaatg ccggtggtac ccagatgggc ttcagggcag attctttcgc ccccatcgca
    36781 tacgtgcagc cgggctcctt tggtgccgct gccggtgccg tcaatgccat tctctcactg
    36841 gcaaaactgg ccggcgtcgg ttttgtcatg aacggcatat ccatctggcg caaatcactg
    36901 ctggacgggc acaccgccct gagcgccagt gaatccatca gtaaagggaa cgtcaagttt
    36961 gtggccggcg ttctgctggt tttcaacgac cgggttatcg atgccactct ggcaagcctc
    37021 ggtatcgcct tctgacccct gtattttttc agtaaatcaa tctgttaata aaggagacac
    37081 tatgtcacga cttcgtgcca tggcggcgcg tctgcgcgct gcatctgttt atgttcagtt
    37141 atgcctgatg gcctgccgtg accggctggt acgcctgtta ctgctgcttc ccgtgatgct
    37201 gctgccccac accgcaatgg caaataccct cttcgatatg attaaaaccg ttggcgatgg
    37261 tgccagaggc tccagcagtt cgtttgtggc tatcggtcag gttattggcc tggtgctctt
    37321 tatcggcggc ctgcttggcc tcaaacaaat cggtaaaaaa cccggtgcag ggctggcctg
    37381 cctgctgtca attctggtcg gcgtctgcct tgccgtcgta ccggaactga tgggcagcgg
    37441 acagaaaacg ctcggtcttt ctccggtttc aatcagttaa tccgcaaagg caccgggaaa
    37501 ccggtgccgt cttcacacaa tgaggaggac cagaccatgt cctgtgagct gccccctttc
    37561 accctgctca gaacagagca cggcaacgtg attgcccgcg acaaccggga taatcaggag
    37621 tttgtgtttg cagagtgtta ctccaccgct atagcagcac tgattctggc tgccgtcacc
    37681 ctgtatacag agagaaaagc gtatgaaaaa gaccccgcca ctcatcgccc cggtccggga
    37741 acgatggctt ttcccgctcc cgcaaccgga cacggtattc agcggtgaag ccctgttacc
    37801 accgtcagtg ctctcaacag agggacgctg cccctgctgt cgcaggaccg tcacccaccg
    37861 cttcattctg gaagattcat ggccattaca gcagatggct gacacctgcc gggatacggt
    37921 cgtcctgctg gaaaaaaacc tcacccgggt gatgcgcctg aaaaaacgtc cggtaccgga
    37981 aaacgctgac gaaaaaaaga aacacacccg aaccctgcag gatgcggagc ggtccctggc
    38041 acaggcccgc ctgtcggcca ggcgactggc cctgcgtcat gtggaaaagt cgcaaatcgt
    38101 cacaacggat gcactcagcg aaaatgaaag tgacctgctg cagccggaag gcccgccctt
    38161 tcatctctgt gccttctgcc atgcctggca ctgtctgaac ggctatgctg ccgcacaggg
    38221 agtgatggtc tggctccccg atttgcatcc ggcctcggtg gttgccctca atgcgcgggc
    38281 actgcaggag attttcagcg ataaccgtca gcgggtcagg cagggacggg cagtcctgaa
    38341 tgccctggta cagaaccgtc tggccgttga ggagaaattc cgcacctggc gtccggcgga
    38401 ttttgctgat gccctgcgcc gctggccgcc ggcacagcgt aaaacgttga gggagaaaat
    38461 ggacggggtg gcgctcattc tgctgccgga ttcgtttcct gacaaaaagt acgtcatgtg
    38521 acacacaccg gtgccggtga cgttttgccg gatatcagtt ctccgcagat gcattccggc
    38581 actttattat ccggtcgctg actgacgata ttccgcctga cggagatgaa aaagagattg
    38641 cccggttcat tcgccgccag cagtttattt atccgggtgt aatcacacag aaatacgcct
    38701 ctgcccctca acatattatt tatcttctct gctgtttttt gatgccacat cccctgctgt
    38761 ggcatcaaaa aatataagac cgtgatgatt catccttacc ggagtgcccc atgccatgaa
    38821 tatcaaccgc attatttttg ccattgaaga ctgtatcagt accctgtcac ggtattccgt
    38881 ttccgcattc ttccccgatt actgtgatct gcacaccgtc atcggtctgg accagcagga
    38941 ccgcgaacgc cgcccctggc tgaaagcccc ctacattgcc accacaaaac aggggtatta
    39001 cctttccgtc tttgaaatca gcggtgccct gaaagaaatg gatgaaaacc cggaccagac
    39061 ggcccccggg acactggagt cactgattgc agcactggct gagcgaatga acacagcctg
    39121 gaaaaattcc ggccacaaaa tcagttttgt tttcgaacgc gatccggagc gtggaaggga
    39181 tgaaatcgaa gccatgctct taccccagcg aaaatccatc gcacgcacgg ggatcctgct
    39241 tcaggacgtc ctggatgaaa aagtcaccac actcaccccc tggcttgtcc gggagcgctg
    39301 ctggctgacc gtctggagct ctgaagaact gctgagccgg accgatcgca aggaccatca
    39361 gacccgtgtc aggaaactcg ctgaacacgc tccgccagcc cgttttgcgc aggatccctg
    39421 gcgctggaca ctttccgccc tcaaaatacg gcatgacgcc ttactggaca cgctggagca
    39481 ggccctgaca catgattctg atggcctgct gattcgcctg atggatatcc atgaagtggg
    39541 acgggaaatc cgccgccagc tggagcgcaa cagtacaccg gcagtatggc agcctcatct
    39601 gcctgaagat gcccgtcctg ccggctggcg acagggcggg gatacctctg tattccacgc
    39661 accgtcgctg aatctgcagt tattcaccac acagcctgaa acacacggca gcctgattca
    39721 ggccggtgag ctctggcacg gtatggtcgc cattaccctg ccccctcaga atctgcggac
    39781 attcaaccag ctggtcaggg atgtgccccg tgcaatcccc tggcgtatcc ggatggatct
    39841 gatgcccgac ggcatgaagg cgctgggggt caagaaaacc ctgctgacct acagcagctt
    39901 tattcctcct ctccggccca tgtatgactc ggtaatgatg ctgaatgaca tcaacaagca
    39961 tgatccggtc tgtatcatga ccattgttgc caccacatgg ggaaacagcc gggaagtctg
    40021 tacccgtaac caggctctcc tgaaatctgc gctggaaggc tggggggtat gcggcaccac
    40081 cacaaccttc ggtgatccga gacgtgcctg ggtgaacacg gttctggcag cctcccacag
    40141 ctccggcccg atcccccttt acccgcccct ttcacacgcc ctttccctgt ttcccctgaa
    40201 cagggcaggc tctgtctggc ggggacaggg aaatctgatg atgcacaccg aagacggctc
    40261 tgcatgggag gtggcgctgg cgtcatcaca acagaacaag cacacggagc tgacacccgg
    40321 cgcacccggt ctgggcaaat cggtactcat caacgcactg tcggagatcc agatagcctc
    40381 agcccagaag aatctccctt tcattgccta tatcgacaag ggattcagtg cacagggact
    40441 ggtacagctt atccgtgaca gcctgcctga gcagagaaag gacgaagccg tggggatcat
    40501 tctgagtaat gacccggacc acacccgcaa cctgtttgat gtgatgtacg gggccagaaa
    40561 acccgtcaca ccggaaaaga acttcatggt ctccgtcctc tgtgcattgt gtgtggatac
    40621 cggcaccggt cagccctgta atcccggcga cacccggcaa atcatcagca gcctggtgga
    40681 cctggccttc cgggaatatg gcgagaataa ccccagactt taccgcgcag gtacggaacc
    40741 cctggtggat ctcgccctgg aagagagcgg catcgctgaa caacatgatg ccggatggtg
    40801 gaatgccgcc acctggtttg aaatcaggga catgctgcat attgcaggga atatccccgc
    40861 cgcccagcgg gcacactacc aggccatgcc cctgctggct gaaatgtccg ccctgctggg
    40921 acagccgtcc atccgggatg tgttcggcac cgttcagcgt gataactcgg aagagcggtt
    40981 gctggactac atccgccgtg ccctcgatca gggacacagt gactacccga tgatgtccgg
    41041 ctgtacccgg tttatgctca gccccgacac ccgcgtggtc gccgttgatc tgaacaatgt
    41101 ggccggggac aagacacctg ccggacgtct gcgcaccggt atcatgtatc tgctggccgg
    41161 gcagatcgcc ggcggggatt ttgtcctgcc gcagtatcag gaggaaatac ggaaaaatct
    41221 cgacccacgc taccatgagg tgattttccg ccgcattgaa cagcttgatc aggaggtcaa
    41281 gaccaaagtc tatgacgaac tgcacaatgc aaaagggatc aacttcatct gggaagccct
    41341 ggacacccag gagctggagc agcgtaagtt cgggatacgc acggtgctga gcacccagct
    41401 gctgcagcat tatcctccgg ccgtgctcaa atccgccaac accctgtggc tgctgcgtta
    41461 ccgccctgac gaaattccgt tcctgcggga taactttggc gtcccggaag tcaccctgcg
    41521 gcgcttcctg aaaatgccgg aaggggccgc accggacggc agcggtgtcc cggtactggc
    41581 tgtgttccgt gtaaaaaacg gcacgctggc ccggatcctg aaattcaccc tcggtccact
    41641 ggagctgtgg gcactgaact cctctcctaa agacagcgcc ctgcgacgtg cgctgacgca
    41701 ggaggtgggg tcactgcggg cacgccagat cctggccgaa cactttcccc ggggttcagc
    41761 cacctcactg atagaacacc gcgcccgcac acatgacagt gaaaacgtca tccatgagct
    41821 ggccgcagaa ctcatccgta agcagggata taacctctga tgtacagcat tcatatcacc
    41881 ccccgctgcc ggcgtcctgc ccggggacag gtcttcctga aaacgctgcg cgtggacggt
    41941 gacagtttca gcttcaccct gccgtccgcc tgcttcacac tgcgaccgtc ccccaatccc
    42001 cgggtcattt ttgccagtga cagacgggct gacggggaga tgagtggtat tcgcctggcc
    42061 ctgcccggag agacaggcac gctgataaca acggccacct ggcaggttaa cgtgccggag
    42121 cgaccgcctt ttgatataca gcatgtcatt cactggcttc tgcccgcacc actcactgcg
    42181 ctgatgtctg acaacacctg gctgtggccg gatgtccccg gcacaccggc actcacagcc
    42241 ggagactggc cggtcatgga aagcgacacc ccgacactga aacgcgtgaa gcgtcgtacc
    42301 ttcacgctca tcgataaccg taaccgtcag ggatggatca cgcgccgctt ccagtgtttc
    42361 ccgctggctc cgctgccgga atcactcagt catcgcgtac cagacatcct gtatcccccg
    42421 aaaacggaga aaaaagaatg caaagacaat ctgcaaccac actgcttgca ctccttgtag
    42481 gctgtgcggt tatccctgcc acccatgcct actctgtgaa tgttgtgtcc agtcagccgg
    42541 tcacccgaca gatcatgccg gagctggcca ccatacaggg catcatggag gaaatggtcc
    42601 gtatgcagac ggccaccggc acagccatca cccagaactc agaaaaactg gccaccgtca
    42661 ttgcccagga cgggcaggcc acccgtcagc agatgatatt cagtaatgaa acacaccgcc
    42721 tcgaggaggc caggaaaagc ttcagcgtgc cggactccat ctgcagtgaa tccgcgtccg
    42781 gcattgcagc agaaagccgc cgggcagcag cgtctgcagc ggcacgtctg agtcaggggg
    42841 gaggggtcag cagtaaaccc atccgggaac gtctctcccg ggctgcggac tccccggtcc
    42901 gcgaggcgta tgacagtgcc ggcatccacg ccggttactg cacggaagcg gagtatgccc
    42961 gctttggcgg aactgatgtc tgccctgccg tgggcgatct gccgggcgga gacagccagg
    43021 tacgatcgct ctatcagggc gcaggcacag cagatacccc ggccgctctc acctgggatc
    43081 agaaacagat tgatgccgcg acggcttata tgaaaaacac cgccagaccg tcagcgggtc
    43141 gtgccccggg gaaaggagaa gtgggcacac agaccgggcg cacgtatgtg ggcctgcaga
    43201 atgaatacaa cggcatcatc gatgccgcct ctcatccgca gctgtcactg attgctgaca
    43261 gtacccctaa tgaagccacc cgtggggcac tgactgaagc cctgcagtca ccgtcagctg
    43321 cggcatattt tgaccggacc gcatcatcag aagcccgcac ccgtggccac atgagccagc
    43381 gggagtttga gacctttgaa gccggcagac ggtatgccaa caccgactgg cagcaggatc
    43441 tgcagggcat ggaaggcgat aaccttctcc gtgagctgct gcgcaccacc gccctgctga
    43501 actggcagat gaacgatctg aaagagcaga ttcgtcaggg taacgttatc gccgggcagc
    43561 aacttgcgct ggccgcacgg cagtattacg gacagcgcct tggagaactg tcacaggcca
    43621 tgagccaggg gagcgtaaga tgaaagacga caacagcatc gacgaaaaaa aaccggcccc
    43681 ccgtctccgc cgcatcggtg atatagggat aaatctgttc gcaccactgg ccgaaacccg
    43741 ctggatgctg cgcagctgcc gccatatgct ggaccagcat aaaacgcgcc tgaagcgcct
    43801 catgcccgac cggcaaccgg agagtccggt ctgtatgacc tgggaggaag cggtcagggc
    43861 cagcggactg acggtcgacg aactggaccg gcgtctgcga cgtcgcagga tgttctggcg
    43921 gacaggattc tggctgatga tgataccggt acttctgctg tgtggtatgg cccttgcggc
    43981 caccgcactg ccggtcatca ccctgatacg tatcctggtc tgcctgttac tgatgagcac
    44041 cattgcagca ctgtgtgcct caaaagccct gattgcgtca taccgcctct ggcagctgaa
    44101 tgaacgtcgg gtcagcgagg acgaacaggg cacattcccc gattacctgc aggcccggca
    44161 cggctggaaa caggccacat ggctggccat tacccaccgg cagtactgac tgtccggtct
    44221 gcaatttctt ttcctcattc cgggggctga tgcccgggca tccccttcgg ttccggagtt
    44281 accgtgaaaa tcatacccag agcactgagt gcaggtctgg cgctggctgc cgtacctgca
    44341 caggctttcg tcacctatca ggacattgtg agtgccgcca ccaatccgga tgacctgtcg
    44401 cgccaggcac tggtcaccat ttttggtgat gtggtcacaa atcccctgac agccagcagc
    44461 aacaccatca ttggcaacct gttcatggtg tttaacgcaa ttatcgccac gatggggata
    44521 gtctggtttc tgtttattgg tatccgtcat gctgtccgca cgggacacca gggcagtgtc
    44581 tttaacaacg gacgggacgt cgtcggcata ctcagtgtcg tgagtggatt tttgatgatt
    44641 gtgcccaccg cttccggctg gagtctggcg caactggtca tgctgtgggg cgcatccatc
    44701 atgggcatcg gttccgccaa tatcatggtt cagacagcgg cagaaaacat tgctgaaggc
    44761 tattcaatga ccgtacagcc agtacatgtc tccacccgca cggcagcacg gggaattctg
    44821 gagatggaac tgtgcaaaca cgccatgaat acgggcctcg atgactttaa ccgtaccgca
    44881 cgttcatcaa cgccgcccat gagcgaatcc tcacggacag agaatggcag atataccgtc
    44941 accgtctcaa atggcagcgc cacctgcggg agtgtgagtc tctccgtgaa aggaaacggc
    45001 agtaacaagc agtccgcgct ggcccatttt ttcaatccct tcattgaaac ggaatatcag
    45061 ggggttctga cggcacaacg ctccgccatg gataccatgc tccgtgacct ggacagcgcg
    45121 gcgcaggaat ttgtgtcggc ataccggacc cggcgcgaca cgggtaacgg caacctgccg
    45181 gatattgaaa gccgtattca gaaggctgcc aacaggtatg aagaaacggt gcagaaggcg
    45241 ttgccaacag ataacgcaga aactgagcgc agggagcagc tgaaatccta cctgaccacc
    45301 tacggctggg ttgccctggg cgcctggtac cagacctttg ccaccgccaa ccagcgactg
    45361 gctgaactgg ccgaccgtgc tcctacagtg agtggcgtct cgtcactggg cgaagccggg
    45421 gatacggaat tcctgaagga agtcaagggg gcttaccagg cacagctaca gaactcagcg
    45481 tatactcctg cactgggaac aaccacctct gccagtgaat accacgcatc taatactcaa
    45541 aatcctcaat cagccctgtt agagttcata gggccagggt taactcgatt ttcaaataaa
    45601 gtagcaacca tacattcagg aacaggaaca acgagtcagc aggcaaatcc tctgctgaag
    45661 atgaaggaca ttggtgatta caccatggtc acaaccgaaa ccatctgggc gggttatacc
    45721 accgtcaggg tgctttctac tgccggcagc aactccgttt ttggccgact ggtcaatgcc
    45781 gtcagcggct tatctgatgc agcaaaggct tttctggaag cctccgcacc accactctac
    45841 ttcctgctgt ttttgctgtt ctgtgccggt ttttcgttat ctatttacct gccgtttatc
    45901 ccgtttatct tctggatgac cggcatcggc aactggatag tcagcgtact gattggatgt
    45961 gctgctggcc ccctgtgggc cgccacccat ctgggaacaa gtcaggacag gggaagccgg
    46021 gccgcttacg gttacatcta cctgatagac agcatgatca gaccgctgct gatggtattc
    46081 gggttctttt ttgcatcggt cgccatcgtg gccggcggca caatactgaa tgcgctgttt
    46141 gctgctgcgc tgatgaatgt ccagataaac tctgtgacgg gcgtgttcag catggcggga
    46201 tttttgatga tttatgcccg agcctgtacc acgctggttg cggccatttt tgcacttcag
    46261 gcttatctgc ctgaccatgt aattaatttt ctgggcgggc gtgacggtgt taatatgctg
    46321 ggaaatatgg tttcttcagt caaagatatt attgccggag gtaataccaa tatgcgcaga
    46381 acacctggcg ttcgggaaga tagactgaaa aatattaata gagatgataa agacggaata
    46441 aaacaataaa atgaaaacat agaaaaacca ataaaacagc attgccatat tcacctgaag
    46501 agtttaaaat atattcaggt gaatatggag gtttattatg aacaaaacaa aaagcagata
    46561 tccttcctgg tgggcatatt accacttggc ccgcaacgct tatttcttat taggaatacc
    46621 gtgtttactt ttatactcag ccgcttatac atcagattta ttagatgata atctaagaca
    46681 ttcaataagt ctttttattc tggcatatgg atgttatttt cttcttttca ttcctccgct
    46741 ctggttatat ctgagaacca gagcgaagaa aaagaaagtc cagcaggttg ttcagcagat
    46801 aaaagaaagc agtccctttg cgccgaccag cgattacgag caactgtcat tctcaaagag
    46861 ttgttatttc gggatcgaca ttaaaaacgg caccatgctg tatgtgcgca tttatccgaa
    46921 caatgtgatg gatgtgattg gtctggatat tcacaacttt acccgcacgg ttgctgagga
    46981 cggcaagctg gaaatccaca ccacctatgt gagtctgccg atgattcctc ttgagatctc
    47041 cggcatctca gcaaggacgc tggccaacac catgcacacc atggctgcac gtggatatga
    47101 gtacaaggaa cgtttcccac agatgatccg gtatcgggtt aaagagtggg aaaaagttgc
    47161 cggtgttcct gttgcagaag tattttgagg tagctatcaa ctgttagacg ataatccgtc
    47221 agccggtttt ctctgaaccg gctttttctt ttcaggcaga atgcctgtct gccgtctatc
    47281 tcaggacgcc gcatggagtc tctatgtttt ccccacaaat tcttagcact gtcttgccca
    47341 ctgtagacaa aatcaataca attctgcata tcagttatct ataaaagata acaatatcat
    47401 tataaataat tatcatatcc acacactcaa ggcagtacct agaaaacata aagataaaat
    47461 catgtctgaa tcccgcagca attttccagc acgacatggt actccatgga atactgaaga
    47521 gttacagcgt cttctcgtat ccatagcgga gggcgtctcg ctaaaaaaac ttactcaaac
    47581 acatcaacga tcgtccgggg gaatacgtgg agcaatcaaa cgcctgcttc cttctgagtt
    47641 tcttgagttc aacgatataa acacggtgaa tgatctcagc aattacttca acaagattcc
    47701 tcgccaagaa cacaatagta tcatcaataa tttaatatca ttgagcactg caaaaacaat
    47761 tgctcatgat aaagttgaaa tacaaaagaa tagttttaaa caaaataccc cactccccct
    47821 ctgccaaccc aatacagatt ttaaagatta tatagccgat gaacatgatg tcattatgct
    47881 aattcattct gctatcgaaa gtttatctaa taaacgacat caatatattc ttctaatgcg
    47941 actcggcaca gatgattcac ctcattctct ggcagatatt gccgtcaaat taggaatttc
    48001 ccgtgaaagg gtaagacaac ttcaggaacg agcccttggc ttactagctg ctaaatctcg
    48061 ctatgaaggg acacctggag cttgccttaa agcccttatt aactcaatag gtaattcacc
    48121 atatgatata gcagtatgga tttatcgtat tgttcatcgg gatttcgtta catcctctga
    48181 cttagccgcc aaattcatta tttttaccgc aggcattcct aaagcaagac gagatgaaat
    48241 aaaaaattcg ctctcattaa tatcacaatt acaaaagcag aagttacgtg aacgaagagc
    48301 agaattagca cacgctcacg ctcaggaaag agcccgaatc aagaaagaaa gagttgagtt
    48361 catattcagt aaatggctag acaatacaca ttggccaaaa tatatcgcac ctcctccccc
    48421 cgtagaacag ctatgttcac agagagcggt taatgatact gacatatctg gattctttta
    48481 ctctcaaaaa ttaggtcgta cagtccagta cgaatcaggg cttgagttta aaataatgaa
    48541 tctattagaa tgttgcgacc aggttctttt ctatcaagaa caaccatttt ctattccata
    48601 ttgttttaaa aacaaatcaa aaaaatacta tccagattta ctcattgcga cagttgatgg
    48661 tcggtgccta cttatggaag tgaagccaac agaccgaatg accttgagtc tcacgagaat
    48721 aaaaagtaat gcaggtcgca aatgggcaca tactcgagga tggggatggt tggtcattag
    48781 tgataggttc tcgttacgtc agctggaaga acatgccatc tcaacaaaca catggaaact
    48841 tatagaggct gaactgaaaa catcaaggat tttagcatgg cgtgaaatga tggagttacg
    48901 tacacgatac gagattcatc gtttagatct tattgcatat atcattcagt ctggtgctga
    48961 agtggataac ttttatcgaa ttaccccgaa aatatctaat aatacatcta accagagaca
    49021 accaaatgag agcgactgtc ataattgatg acgcacagga cggaaaaaat aatagctgcc
    49081 ggacagcagc aaccagcagt cgtccggtaa aaaagacaaa caaggcgcat tgaggtcagc
    49141 cttcgcaaca atccggcggc agatgtcctg ccgccgtgtt gcccgttaac gtttaacttc
    49201 gtaggctaac gttgccacaa acaccgtatt tccctgctcg atacgaaatc cgcaaagcga
    49261 atccctcacc atccagacaa aacacagtat cgtcacacag acgacgatta acatcattaa
    49321 aaacgttcgt tgtggcatcg tagcccctcc attgcctttc ggccgtcatc gggctactat
    49381 aatgttgtct aggcattaca gtgggccctg ggttgattga taaaaaaatc gcctggggct
    49441 ttctgctaca tgccgtccac aaaacgcttt cggcatacag cctcaagcgc ccgccgctat
    49501 tctaatcgtt ttcctgtccg ccacaatcct tttcatgcca ccacgtattt tgtgtcaggg
    49561 acagaaataa agccggaaag tgactacgtg tgcacacatc tgttttccgg gctgcaggca
    49621 tcgtgttatt ttctgaaatg caaaataata aaaaacaagg acacatcatg aaggatgaaa
    49681 tacacagtac cagcattaac ccggatgttg ttgagcagtt tctggccctt atgaatacac
    49741 attgtaaatc ctcagaagag gcgctcacca tgctgagtac gctttgttac aacctgatga
    49801 tgacatgcgg tatccattcc atggacagcc agatgctcaa tgggcatggg atatccatgc
    49861 aacttcaggg g
//
LOCUS       DAZPDU010000006        49303 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZPDU010000006 DAZPDU010000000
VERSION     DAZPDU010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49303
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.006"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            274..1368
                     /gene="gcvT"
                     /locus_tag="ACHURS_000317"
     CDS             274..1368
                     /gene="gcvT"
                     /locus_tag="ACHURS_000317"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFR1236144.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVLEGI
                     GETAIVQIRNREMSVKVTKPVFVRNGKAVA"
     gene            1392..1781
                     /gene="gcvH"
                     /locus_tag="ACHURS_000318"
     CDS             1392..1781
                     /gene="gcvH"
                     /locus_tag="ACHURS_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFR1236145.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            1900..4773
                     /gene="gcvP"
                     /locus_tag="ACHURS_000319"
     CDS             1900..4773
                     /gene="gcvP"
                     /locus_tag="ACHURS_000319"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFR1236146.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAAVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            5039..5782
                     /locus_tag="ACHURS_000320"
     CDS             5039..5782
                     /locus_tag="ACHURS_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR1236147.1"
                     /translation="MAIALVTDGSRGIGRATALLLAKEEYTVAVNYQQNLHAAQEVVN
                     LITQAGGKAFVLQADISDENQVIAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNMTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGVRVNCVRPGFIFTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(5839..7272)
                     /gene="bglA"
                     /locus_tag="ACHURS_000321"
     CDS             complement(5839..7272)
                     /gene="bglA"
                     /locus_tag="ACHURS_000321"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFR1236148.1"
                     /translation="MKKLTLQKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVITSNGEKL"
     gene            7317..7628
                     /gene="yqfB"
                     /locus_tag="ACHURS_000322"
     CDS             7317..7628
                     /gene="yqfB"
                     /locus_tag="ACHURS_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFR1236149.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            7792..8451
                     /locus_tag="ACHURS_000323"
     CDS             7792..8451
                     /locus_tag="ACHURS_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFR1236150.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(8647..9627)
                     /gene="ygfZ"
                     /locus_tag="ACHURS_000324"
     CDS             complement(8647..9627)
                     /gene="ygfZ"
                     /locus_tag="ACHURS_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFR1236151.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDEHVLLGVAGFQARAALANIFSELPSKEKQVVKEGATTLLWFEYPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            9870..10136
                     /gene="sdhE"
                     /locus_tag="ACHURS_000325"
     CDS             9870..10136
                     /gene="sdhE"
                     /locus_tag="ACHURS_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFR1236152.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            10117..10524
                     /locus_tag="ACHURS_000326"
     CDS             10117..10524
                     /locus_tag="ACHURS_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFR1236153.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(10564..11085)
                     /gene="fldB"
                     /locus_tag="ACHURS_000327"
     CDS             complement(10564..11085)
                     /gene="fldB"
                     /locus_tag="ACHURS_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFR1236154.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            11197..12093
                     /gene="xerD"
                     /locus_tag="ACHURS_000328"
     CDS             11197..12093
                     /gene="xerD"
                     /locus_tag="ACHURS_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFR1236155.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            12118..12828
                     /gene="dsbC"
                     /locus_tag="ACHURS_000329"
     CDS             12118..12828
                     /gene="dsbC"
                     /locus_tag="ACHURS_000329"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFR1236156.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     ALQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            12834..14567
                     /gene="recJ"
                     /locus_tag="ACHURS_000330"
     CDS             12834..14567
                     /gene="recJ"
                     /locus_tag="ACHURS_000330"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFR1236157.1"
                     /translation="MKQQIQLRRRAVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEADKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            14658..15756
                     /gene="prfB"
                     /locus_tag="ACHURS_000331"
     CDS             join(14658..14732,14734..15756)
                     /gene="prfB"
                     /locus_tag="ACHURS_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFR1236158.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            15766..17283
                     /gene="lysS"
                     /locus_tag="ACHURS_000332"
     CDS             15766..17283
                     /gene="lysS"
                     /locus_tag="ACHURS_000332"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFR1236159.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(17326..17874)
                     /gene="idi"
                     /locus_tag="ACHURS_000333"
     CDS             complement(17326..17874)
                     /gene="idi"
                     /locus_tag="ACHURS_000333"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFR1236160.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            17929..18000
                     /locus_tag="ACHURS_000334"
     CDS             17929..18000
                     /locus_tag="ACHURS_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1236161.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(17997..18122)
                     /gene="yqfG"
                     /locus_tag="ACHURS_000335"
     CDS             complement(17997..18122)
                     /gene="yqfG"
                     /locus_tag="ACHURS_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFR1236162.1"
                     /translation="MNFLMRVIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(18124..19202)
                     /locus_tag="ACHURS_000336"
                     /pseudo
     CDS             complement(18124..19202)
                     /locus_tag="ACHURS_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
     gene            19638..21557
                     /gene="ygfT"
                     /locus_tag="ACHURS_000337"
     CDS             19638..21557
                     /gene="ygfT"
                     /locus_tag="ACHURS_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFR1236163.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQWIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            21557..22045
                     /locus_tag="ACHURS_000338"
     CDS             21557..22045
                     /locus_tag="ACHURS_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR1236164.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(22114..22464)
                     /gene="ghxQ"
                     /locus_tag="ACHURS_000339"
                     /gene_synonym="ygfQ"
                     /pseudo
     CDS             complement(22114..22464)
                     /gene="ghxQ"
                     /locus_tag="ACHURS_000339"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
     gene            complement(22500..22814)
                     /locus_tag="ACHURS_000340"
     CDS             complement(22500..22814)
                     /locus_tag="ACHURS_000340"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFR1236165.1"
                     /translation="MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGN
                     FLPGKEADFVVMEPTATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYE
                     RN"
     gene            complement(22747..25686)
                     /locus_tag="ACHURS_000341"
     CDS             complement(22747..25686)
                     /locus_tag="ACHURS_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFR1236166.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLDVIGKHYPKTDAAKMVQAKPCYVEDCVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEAVADVQLATPGVVRGKKGEVSFGDIAHKGETGIGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWAEESQSGHGNGYRCRNNFQHAANAERSLQSVAIT
                     RLSPLGI"
     gene            complement(25683..26462)
                     /gene="ygfM"
                     /locus_tag="ACHURS_000342"
     CDS             complement(25683..26462)
                     /gene="ygfM"
                     /locus_tag="ACHURS_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFR1236167.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(26513..27841)
                     /gene="ssnA"
                     /locus_tag="ACHURS_000343"
     CDS             complement(26513..27841)
                     /gene="ssnA"
                     /locus_tag="ACHURS_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFR1236168.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSELSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARLIDEAKKATSEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(27844..30942)
                     /gene="ygfK"
                     /locus_tag="ACHURS_000344"
     CDS             complement(27844..30942)
                     /gene="ygfK"
                     /locus_tag="ACHURS_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFR1236169.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMLQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRENLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMVLAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCNKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYEDKITVFSLAQDFDNSS
                     TPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(31264..31842)
                     /gene="mocA"
                     /locus_tag="ACHURS_000345"
     CDS             complement(31264..31842)
                     /gene="mocA"
                     /locus_tag="ACHURS_000345"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFR1236170.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMFPWEQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     DHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
     gene            31945..32715
                     /gene="yqeC"
                     /locus_tag="ACHURS_000346"
     CDS             31945..32715
                     /gene="yqeC"
                     /locus_tag="ACHURS_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFR1236171.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            32763..34388
                     /gene="yqeB"
                     /locus_tag="ACHURS_000347"
     CDS             32763..34388
                     /gene="yqeB"
                     /locus_tag="ACHURS_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFR1236172.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVEWKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EELDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILRVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTDVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(34429..35361)
                     /gene="arcC"
                     /locus_tag="ACHURS_000348"
     CDS             complement(34429..35361)
                     /gene="arcC"
                     /locus_tag="ACHURS_000348"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFR1236173.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVIAVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(35409..36794)
                     /gene="hyuA"
                     /locus_tag="ACHURS_000349"
     CDS             complement(35409..36794)
                     /gene="hyuA"
                     /locus_tag="ACHURS_000349"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFR1236174.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(36847..38058)
                     /locus_tag="ACHURS_000350"
     CDS             complement(36847..38058)
                     /locus_tag="ACHURS_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFR1236175.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(38116..39312)
                     /gene="dpaL"
                     /locus_tag="ACHURS_000351"
     CDS             complement(38116..39312)
                     /gene="dpaL"
                     /locus_tag="ACHURS_000351"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFR1236176.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQHQSLMEKLALDKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(39370..40557)
                     /gene="ygeW"
                     /locus_tag="ACHURS_000352"
     CDS             complement(39370..40557)
                     /gene="ygeW"
                     /locus_tag="ACHURS_000352"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFR1236177.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            41036..42814
                     /locus_tag="ACHURS_000353"
     CDS             41036..42814
                     /locus_tag="ACHURS_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFR1236178.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQFEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTSASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(42854..43333)
                     /gene="xdhC"
                     /locus_tag="ACHURS_000354"
     CDS             complement(42854..43333)
                     /gene="xdhC"
                     /locus_tag="ACHURS_000354"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFR1236179.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(43330..44208)
                     /gene="xdhB"
                     /locus_tag="ACHURS_000355"
     CDS             complement(43330..44208)
                     /gene="xdhB"
                     /locus_tag="ACHURS_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFR1236180.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLADGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEAPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(44219..46516)
                     /gene="xdhA"
                     /locus_tag="ACHURS_000356"
     CDS             complement(44219..46516)
                     /gene="xdhA"
                     /locus_tag="ACHURS_000356"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFR1236181.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGRN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFAPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPSAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            46931..47686
                     /gene="actS"
                     /locus_tag="ACHURS_000357"
     CDS             46931..47686
                     /gene="actS"
                     /locus_tag="ACHURS_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFR1236182.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            47765..47834
                     /locus_tag="ACHURS_000358"
     tRNA            47765..47834
                     /locus_tag="ACHURS_000358"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:47797..47799,aa:Gly,seq:ccc)
     gene            48039..>49303
                     /locus_tag="ACHURS_000359"
     CDS             48039..>49303
                     /locus_tag="ACHURS_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HFR1236183.1"
                     /translation="MKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTGAATD
                     KSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDEIQGLLTLTENTRKLTLTEKPWGT
                     FILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYMSTLD
                     QIKRMQDVISNPKRLDGVPGQMNAVHIRFDSNSPVMADVQEEGKPQLWNHKQNDALGL
                     YLDLLIQAIDTGTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDARLNTS
                     SVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYERITL
                     QLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKKLAGPYGI
                     KRYEKDNYQSANFWFNDIKTDTDQNSHAK"
BASE COUNT        11962 a        12176 c        13007 g        12158 t
ORIGIN      
        1 attgccttat tcaaattagc tttctaatgg catcatcttt atatagaaac tggtgtgttt
       61 tttggttttt tattctgtcg cgattttggc gttttttaac cataagctaa tgtgatgatc
      121 aattttacct tatggttaac agtctgtttc ggtggtaagt tcaggcaaaa gagaacgatt
      181 gcgttgggga ccgggagtgg ctccgatgct gggtttcgtg gtgataattt caccatgaaa
      241 agttgtcagc cccgcttatt caatgaggac aagatggcac aacagactcc tttgtacgaa
      301 caacacacgc tttgcggcgc tcgcatggtg gatttccacg gctggatgat gccgctgcat
      361 tacggttcgc aaatcgacga acatcatgcg gtacgtaccg atgccggaat gtttgatgtg
      421 tcacatatga ccatcgtcga tctccgcggc agccgcaccc gggagtttct gcgttatctg
      481 ctggcgaacg atgtggcgaa gctcaccaaa agcggcaaag ccctttactc ggggatgttg
      541 aatgcctctg gcggtgtgat agatgacctc atcgtctact actttactga agatttcttc
      601 cgcctcgttg ttaactccgc cacccgcgaa aaagacctct cctggattac ccaacacgct
      661 gaacctttcg gcatcgaaat taccgttcgt gatgaccttt ccatgattgc cgtgcaaggg
      721 ccgaatgcgc aggcaaaagc tgccacactg tttaatgacg cccagcgtca ggcggtggaa
      781 gggatgaaac cgttctttgg cgtgcaggcg ggcgatctgt ttattgccac cactggttat
      841 accggtgaag cgggctatga aattgcgctg cccaatgaaa aagcggccga tttctggcgt
      901 gcgctggtgg aagcgggtgt taagccatgt ggcttgggcg cgcgtgacac gctgcgtctg
      961 gaagcgggca tgaatcttta tggtcaggag atggacgaaa ctatttctcc tttagccgcc
     1021 aacatgggct ggactatcgc ctgggaaccg gcagatcgtg actttatcgg tcgtgaagcc
     1081 ctggaagtgc agcgtgagca tggtacagaa aaactggttg gtctggtgat gaccgaaaaa
     1141 ggcgtgctgc gtaatgaact gccggtacgc tttaccgatg cgcagggcaa ccagcatgaa
     1201 ggcattatca ccagcggtac tttctccccg acgctgggtt acagcattgc gctggcgcgc
     1261 gtgctggaag gtattggcga aacggcgatt gtgcaaattc gcaaccgtga aatgtcggtt
     1321 aaagtgacaa aacctgtttt tgtgcgtaac ggcaaagccg tcgcgtgatt tacttttttg
     1381 gagattgatt gatgagcaac gtaccagcag aactgaaata cagcaaagaa cacgaatggc
     1441 tgcgtaaaga agccgacggc acttacaccg ttggtattac cgaacatgct caggagctgt
     1501 taggcgatat ggtgtttgtt gacctgccgg aagtgggcgc aacggttagc gcgggcgatg
     1561 actgcgcggt tgccgaatca gtaaaagcgg cgtcagacat ttatgcgcca gtaagcggtg
     1621 aaatcgtggc ggtaaacgac gcactgagcg attccccgga actggtgaac agcgaaccgt
     1681 atgcaggtgg ctggatcttt aaaatcaaag ccagcgatga aagcgaactg gaatcactgc
     1741 tggatgcgac cgcatacgaa gcattgttag aagacgagta acggctttat tcctcttctg
     1801 cgggagagga tcagggtgag gaaaatttat gcctcaccct cactctcttc gtaaggagag
     1861 aggttcacaa ttcactgcac gtttcaggaa ccatcgctca tgacacagac gttaagccag
     1921 cttgaaaaca gcggcgcttt tattgaacgc catatcggac cggacgccgc gcaacagcaa
     1981 gaaatgctga atgccgttgg cgcacaatcg ttaaacgcgc tgaccggcca gattgtaccg
     2041 aaagatattc agcttgcgac cccgccgcag gttggcgcac cggcgaccga atacgccgca
     2101 ctggcagaac tcaaggctat tgccagtcgc aataaacgct tcacgtctta catcggcatg
     2161 ggttacaccg ccgtgcagct accgccggtt atcctgcgta acatgctgga aaatccgggc
     2221 tggtataccg cgtacactcc gtatcaacct gaagtctccc agggccgcct tgaagcactg
     2281 ctcaacttcc agcaggtaac gctggatttg actggactgg atatggcctc tgcttctctt
     2341 ctggacgagg ccaccgctgc cgccgaagca atggcgatgg cgaaacgcgt cagcaaactg
     2401 aaaaatgcca accgcttctt cgtggcttcc gatgtgcatc cgcaaacgct ggatgtggtc
     2461 cgtactcgtg ccgaaacctt tggttttgaa gtgattgtcg atgacgcgca aaaagtgctc
     2521 gaccatcagg acgtcttcgg cgtgctgtta cagcaggtag gcactaccgg tgaaattcac
     2581 gactacactg cgcttattag cgaactgaaa tcacgcaaaa ttgtggtcag cgttgccgcc
     2641 gatattatgg cgctggtgct gttaactgcg ccgggtaaac agggcgcgga tattgttttt
     2701 ggttcggcgc aacgcttcgg cgtgccgatg ggctacggtg gcccacacgc ggcattcttt
     2761 gcggcgaaag atgaatacaa acgctcaatg ccgggccgta ttatcggtgt atcgaaagat
     2821 gcagctggca ataccgcgct gcgcatggcg atgcagactc gcgagcaaca tattcgtcgt
     2881 gagaaagcga actccaacat ttgtacttcc caggtactgc tggcaaacat cgccagcctg
     2941 tatgccgttt atcacggccc ggttggcctg aaacgtatcg ctaaccgcat tcaccgtctg
     3001 accgatattc tggcggcggg cctgcaacaa aaaggtctga aactgcgcca tgcgcactat
     3061 ttcgacacct tgtgtgtgga agtggccgac aaagcgggcg tactgacgcg tgccgaagcg
     3121 gctgaaatca acctgcgtag cgatatcctg aacgccgtgg ggatcaccct tgatgaaacc
     3181 accacgcgtg aaaacgtaat gcagcttttc agcgtgctgc tgggcgataa ccacgggctg
     3241 gagatcgaca cgctggacaa agacgtggct cacgacagcc gctctatcca gcctgcgatg
     3301 ctgcgcgacg acgaaatcct cacccatccg gtgtttaatc gctaccacag cgaaaccgaa
     3361 atgatgcgct atatgcactc gctggagcgt aaagatctgg cgctgaatca ggcgatgatc
     3421 ccgctgggtt cctgcaccat gaaactgaac gccgccgccg agatgatccc aatcacctgg
     3481 ccggaatttg ccgaactgca cccgttttgc ccgccggaac aggctgaagg ttatcagcag
     3541 atgattgcgc agctggctga ctggctggtg aaactgaccg gttacgacgc cgtttgtatg
     3601 cagccgaact ccggcgcaca gggcgaatac gcgggcctgc tggcgattcg tcattatcat
     3661 gaaagccgca acgaagggca tcgcgatatc tgcctgatcc cggcttctgc gcacggaact
     3721 aaccccgctt ctgcacatat ggcaggaatg caggtggtgg ttgtggcgtg tgataaaaac
     3781 ggcaacatcg atctgactga tctgcgcgcg aaagcggaac aggcgggcga taacctctcc
     3841 tgtatcatgg tgacttatcc ttctacccac ggcgtgtatg aagaaacgat ccgtgaagtg
     3901 tgtgaagtcg tgcatcagtt cggcggtcag gtttaccttg atggcgcgaa catgaacgcc
     3961 caggttggca tcacctcgcc gggctttatt ggcgcggacg tttcgcacct caacctgcat
     4021 aaaactttct gcattccgca cggcggtggt ggtccgggta tgggaccgat cggtgtgaaa
     4081 gcgcatctgg caccgtttgt accgggtcat agcgtggtgc aaatcgaagg catgttaacc
     4141 cgtcagggcg cagtttctgc ggcaccgttc ggtagcgcct ctatcctgcc aatcagctgg
     4201 atgtacatcc gcatgatggg cgcagaaggg ctgaaaaaag caagccaggt ggcaatcctc
     4261 aacgccaact atattgccag ccgcctgcag gatgccttcc cggtgctgta taccggtcgc
     4321 gacggtcgcg tggcgcacga atgtattctc gatattcgcc cgctgaaaga agaaaccggc
     4381 atcagcgagc tggatattgc caagcgcctg atcgactacg gtttccacgc gccgacgatg
     4441 tcgttcccgg tggcgggtac gctgatggtt gaaccgactg aatctgaaag caaagtggaa
     4501 ctggatcgct ttatcgacgc gatgctggct atccgcgcag aaattgacca ggtgaaagcc
     4561 gctgtctggc cgctggaaga taacccgctg gtgaacgcgc cgcacattca gagcgaactg
     4621 gtcgccgagt gggcgcatcc gtacagccgt gaagttgcgg tattcccggc aggcgtggca
     4681 gacaaatact ggccgacggt gaaacgtctg gatgatgttt acggcgaccg taacctgttc
     4741 tgctcctgcg taccgattag cgaataccag taattcactg attcgactat tttctaaagg
     4801 cgcttcggcg cctttttagt cagatgacaa agtacaaaag tgctcagaca gtcccctcgc
     4861 ccctttgggg agagggttag ggtgagggga acaggccggc actggcgcga atatttaccc
     4921 tcaccccggc cctctccctg aaagggcgag gggaaaagcg tgccaacatt gaagattgag
     4981 ccagtttgtt agcaatctca aagatacgtc aacgaattaa tttttctcgg aaaaacaaat
     5041 ggctatagca cttgtgaccg atggcagtcg cggcatcggg cgggcaactg cattactgtt
     5101 ggcgaaagaa gagtatacgg tggcggttaa ttatcaacaa aatctccatg cggcgcagga
     5161 agtggtgaac ttaatcacgc aagccggtgg caaggcattc gtgctccagg cggatatcag
     5221 cgacgaaaat caggtcatcg caatgtttac agcaatcgat cagcacgatg aaccgctagc
     5281 agcgctggtc aataacgccg ggatcttgtt tacccagtgc accgttgaaa accttaccgc
     5341 agagcgaatc aaccgagtac tttccaccaa catgacggga tattttctct gctgccgcga
     5401 ggcggtaaaa cgcatggcgc ttaaaaatgg tggcagtggc ggcgctatcg tcaatgtctc
     5461 ttcggtggcc tcacggttgg gttcgccagg ggaatatgtt gattacgcgg catcgaaagg
     5521 ggcgattgat acgttaacca ccggactatc gctggaagtc gccgcgcagg gggtccgcgt
     5581 taactgcgtg cggccagggt ttatttttac cgaaatgcac gccagcggcg gcgagcctgg
     5641 acgcgtcgat cgcgttaagt cgaacatccc catgcagcgt ggtggacagg cagaagaggt
     5701 cgcgcaggcc attgtctggc tactaagtga taaagcctct tacgtcacgg gaagttttat
     5761 cgatttggcg ggcgggaaat aaaacaggga agttgtctga ccggatgcaa caagtattgc
     5821 atccggtact tcatcgactt aaagcttctc gccgttgctg gtaatcacct ctttgtacca
     5881 gttaaagctc ttcttacgtg aacgcgacat atcgccagta ccgtcgtcat gtttattcac
     5941 atagataaag ccgtagcgtt tgctgtactg cccggtggtg aacgatacgc agtcgatgca
     6001 gccccacggt gtgtagccca tcagatccac gccatcgtaa gtcaccgctt ttttcatctc
     6061 ttcgatatgg gcgcgcaggt agtcaatgcg gtagtcgtcg ttgatgctgc catcttcttc
     6121 cactttgtcg taagcgccaa aaccgttttc gacaataaac agcggcctct gataacgctc
     6181 atacagttcg caaagggcat agcgcagacc tactggatca atctgccagc cccagtcaga
     6241 cgctttaaca tacgggttcg gtacgctgcc ttcgaaacca gagatcgcat cgccggtgcc
     6301 gccttcggcc ttcactgcgt tggtcatgta atagctgaaa ccaagataat cgcaggtgcc
     6361 ttcgcgcagt acatcgagat cgccgtcttc cattttgatg ttaaatccgc gacgctccca
     6421 ctcgttcaac acataggacg ggtaatagcc gcgtagctgc acatcggtaa agacgtagcg
     6481 ttcgcgcatc gactcctgag cgaacatcac atcgtccggg ttacagcagt agggatagag
     6541 cggcaccatc gccagcatac agccgacttt catctccggg ttaatgcgac gcgcagcttt
     6601 caccgccagg gcgctggcga caaactggtg atgcagcacc tgatacatcg tctcttccgg
     6661 gttctcatgc tcggtataaa ccacgccgga gcagcagtaa ccgaacagcg gtgcacgcca
     6721 gttacgctgg ttgttaattt cgttgaaggt catccagtat ttgactttat gcttatagcg
     6781 ttcaaacacc acttccgcga aacggacaaa gaaatcaacc actttacggt tggtccagct
     6841 accgtattgc tgcaccagat gcagcggcat ttcaaagtgg gagagggtga tcaccggttc
     6901 gatgttgtat ttcagcagtt catcgaacat atcatcgtag aacttcagcc cttcttcgtt
     6961 tggctgagct tcatcgcctt ttggaaaaat gcgcgtccag gctatggaag tccgaaaaca
     7021 tttgaagccc atttcggcaa atagcttgat gtcttccttg tagtgaccat aaaaatcaac
     7081 ggcttcatgg tttggatagt atttgcctgg caagacttct ttggtgattt cgcgcggcac
     7141 gccgtgtgcg ccaccggtca gaacgtcaca aatgctcggc cctttgccgc ctttgttcca
     7201 gccgccttcg acctgatgag cggcaactgc gccgccccat aagaaatctt tctgtaaggt
     7261 gagttttttc actatcatag gctccagatg agtcaattct tacaggaaat agtaccatgc
     7321 agccaaacga catcactttt tttcaacgtt tccaggatga cattctggct gggcgtaaaa
     7381 ccatcaccat ccgcgacgag tctgaatcgc acttcaaaac gggtgatgtg cttcgtgtcg
     7441 ggcgttttga agatgacggt tatttttgca cgattgaagt caccgcaacc tcaaccgtaa
     7501 cgctggatac gctgacggaa aaacatgcag agcaggaaaa tatgaccctg actgaactga
     7561 taaaggtcat tgccgacatc tatcccggtc agacacagtt ttatgtgatt gaatttaaat
     7621 gtctttaaat tgaactcacg cctgttagtt gaaatttcgc atttaatttc atgattttta
     7681 ggagattagt ctcaaatcag aatttatttt agctaacagg tgttcactgg aactattctc
     7741 agttacgctg gagaggtata catgtgcgtg tacgtttctc cggagtaagt tatggttcag
     7801 aagcccctca ttaagcaggg atattcgctg gcagaggaaa ttgccaacag cgtcagtcac
     7861 ggcattgggt tggtgtttgg tatcgttggg ctggtgttgc tactggttca ggcggtggat
     7921 ctcaatgcca gcgccacagc gataaccagt tacagcctct atggcggcag tatgatcctg
     7981 ctgttcctcg cttcgacgct ctatcacgcc attcctcatc aacgggcaaa aatgtggctg
     8041 aagaaatttg accattgcgc tatttacctg ttgattgccg gaacctacac gccgtttttg
     8101 ctggtggggc tggattctcc gttagcgcgc gggttgatga ttgttatctg gagcctggca
     8161 ttgctgggta ttctgtttaa actgaccatc gcgcaccgat tcaaaatttt atctctggtg
     8221 acctatctgg cgatgggctg gctgtcgctg gtggtaattt atgaaatggc agttaagctc
     8281 gcggcgggca gcgttacctt attggcggta ggcggcgtgg tttattcgct cggagtgatt
     8341 ttctatgtct gcaaacgcat tccctacaat catgccatct ggcacggctt cgtgctcggc
     8401 ggcagtgtgt gccactttct ggcgatctat ttgtatattg ggcaggcgta atgggtatgc
     8461 ctttgtaggc ctgaaaagac gcgtcagcgt cgcatcaggc atcggagcac aactgccgga
     8521 ttcggcgtga acgccttatc cgatctacgg gcctcgtccc tgttgtgata aaggcatcaa
     8581 atcccagaca tgataagacg cgtcagcgtc gcatcaggcg ataaatgcct gatgcgataa
     8641 agagatttac tcttcgagcg aatacggcag cggctcaata tgcaatgtat tcgcatcgtc
     8701 gcgtacgcgg aagatgctat ccggttccat atcgttattc atgacgacct gtaccacgac
     8761 ctgaccatct tccagtttta ccgcagccag caccgtaccg gtacgacgcc agttctcgcc
     8821 cattttcagc tctaagtctt caccagcttc cggcagtcgg ctggcgctac ctgccagcaa
     8881 ccagagcgca cgtttattgg caccacggaa ttttgctcgc gccaccatct cttgtccggt
     8941 ataacagcct ttcttaaagc tgataccgcc cagcgcctgg agattggtcg cctgtgggat
     9001 aaactgcccg ctgttggcgg catcaatcac cgggaaaccc gcttcaatgt ttaatgccag
     9061 ccactgttgg ctattgttca gttccgcttc accgcgcagt ttatcggtca gcatattagc
     9121 agtagcttca tcggttacga tcaggaaacg ttctgccggg tattcaaacc atagcaaagt
     9181 ggtcgcgcct tctttaacta cctgtttttc tttcgaaggc agttcactaa agatatttgc
     9241 cagcgcggcg cgcgcctgaa aaccggcaac accaagcagc acatgctcgt cgtctggcgc
     9301 gatggtcact ttagagaaca ccgcatattt tttcagttca gtcagctgcg gttcacgcac
     9361 gctgcgccgt tcaatccacg caaagccatc gccgtcgcgg aacagacgta aattgctcca
     9421 cattttgcct ttggcgtcgc aatgggcggc gagcaggtgc tgatcttctg ccatctggct
     9481 gacatctgct gtcacctgac cctgcatata tttttcgctg tccgcgccag taatggtggc
     9541 aagcgcccag tcatcaagcg tcatcagcgt cagcggtaaa cgggcagaag ccgtaggctg
     9601 acggggagga aaaggtgtaa aagccataat agagtcctga gttgcttaac ggcgtattaa
     9661 tgactaatgg taaaagagct atgaggcaat gcaagtgctt tcactacgcg atctgtgcct
     9721 tctgcgaggc gacttccaaa aaaatgaagc cattgaattg ttgtgaatta atttcgtgat
     9781 cgcgatggtg tttactgata ttcccgtaaa agaaggcgaa aaacacgtta caatagcggg
     9841 gtactggatt ttcgtgagac aggaagaaga tggacattaa caacaaagcc cgcattcatt
     9901 gggcatgccg ccgtggtatg cgcgaactcg atatttcaat catgccgttt ttcgaacatg
     9961 agtacgacag cttaagcgat gacgaaaaac gcatctttat tcgtctgctg gaatgtgacg
    10021 atccggacct gtttaactgg ctgatgaatc acggtaaacc ggccgatgca gaactggaaa
    10081 tgatggtccg actcatccag acacggaacc gggaacgtgg tcctgtggca atctgatttg
    10141 cgcgtctcct ggcgcgcaca gtggctttcc ttgctgattc atgggctggt tgccgctgtt
    10201 attttactca tgccctggcc actcagttac accccgttat ggatggtgtt actttcgctg
    10261 gtggtgtttg attgcgttcg cagccagcgg cgtattaatg ctcgccaggg ggaaattcgc
    10321 ttgttgatgg acgggcgttt gcgttggcaa gggcaggagt ggagcatcgt caaagcaccg
    10381 tggatgatta agagcggcat gatgctgcgt ttacgttctg atggcggtaa acggcaacat
    10441 ttatggctgg cagccgacag catggacgaa gccgaatggc gggatttacg gcggattttg
    10501 ttgcaacaag agacgcaaag ataaagatga cggcaaagca tatgcgctaa gccgtcagtg
    10561 agatcaggcg taatgctctg ccatttcgtt gaggatttgc tcgcaccagc tctgaatacg
    10621 ctcgtcgcta aggtcatact ggttagtttc atccagcgcc agacccacga acagttgccc
    10681 gtcagcaatc accggtttcg ggctggtaaa ttcatatcct tccgttggcc agtagccgac
    10741 gaacttcacg cctttggtcg agagtttgtc atgcagcata ccgagcgcat cgaggaacca
    10801 ctcgccgtat cccagttgat cgccaagccc atacagcgca acaattttac cttcaaggtt
    10861 caggtcgtcg agctgatccc agacggcttc ccagtcttcc tggatttcac caaaatccca
    10921 ggtcgggata cccagaatga gcacatcgta ctgctccatt aatttcgggg agtcgtcctt
    10981 gaggttatgt aaggtcacca gttctgggcc gataatatcg cggatttttt ctgccgccat
    11041 ttcggtgtaa caggtgctgg aaccgtaaaa aagacccata ttcataacgt aagcttctcg
    11101 attctgctgt ggcttttgca gctagtgtat cagaatcgct ttagtcacgg cacaataagg
    11161 cataatggat caaatgagtg accataaggg gcgcaagtga aacaggatct ggcacgcatc
    11221 gagcagtttc ttgatgctct gtggctggaa aaaaatctgg ctgaaaatac gttgaacgct
    11281 taccgtcgcg atctgtcaat gatggtggag tggttgcatc accgcgggtt gacgctggcg
    11341 acggcgcaaa gtgacgattt gcaggcatta ctggcagaac ggctggaggg cgggtataaa
    11401 gccaccagct cagcgcgttt gctgagtgca gtgcgccgat tgttccagta tctttatcgc
    11461 gaaaagtttc gtgaagacga tcccagtgcg catctcgctt caccgaaatt gccccagcgt
    11521 ttgccaaaag atttaagcga agcgcaggtc gaacgtttat tacaggcgcc attaattgat
    11581 cagccactgg agctacgcga taaagccatg cttgaagtgt tgtatgctac cgggctgcgt
    11641 gtctctgaac tggtcgggct gacaatgagt gatatcagcc tgcgtcaggg cgtagtgcgg
    11701 gtcattggta aaggcaacaa agagcgcctg gtaccgttag gtgaagaggc ggtttactgg
    11761 ctggaaacct atctggaaca tgggcgtccg tggctgctga atggtgtgtc aattgacgtg
    11821 ttgtttccca gccagcgtgc gcagcagatg acgcgacaga ccttctggca ccgcattaaa
    11881 cattatgccg tgctggcggg tatcgacagc gaaaagctgt caccgcatgt gttgcgtcac
    11941 gcttttgcca ctcatttatt aaatcatggt gcggatttac gcgtggtgca gatgctactg
    12001 ggccacagcg atctttccac cacgcaaatt tatacgcatg tcgctaccga gcgtctgcgg
    12061 caacttcatc aacagcatca cccgcgggcg tgatgtctga aaagaacggg aagatttatg
    12121 aagaaaggtt ttatgttgtt tactttgtta gcggcgtttt caggttttgc tcaggctgat
    12181 gacgcggcaa ttcaacaaac gttagccaaa atgggcatca aaagcagcga tattcagccc
    12241 gcgcccgtag ctggcatgaa gacagttctg actaacagcg gcgtgttgta catcaccgat
    12301 gatggtaaac atatcattca ggggccaatg tatgacgtta gcggcacggc tccggtcaat
    12361 gtcaccaata agatgctgtt aaagcagttg aatgcgcttg aaaaagagat gatcgtttat
    12421 aaagcgctgc aggaaaaaca cgtcatcacc gtgtttactg atattacctg tggttactgc
    12481 cacaaactgc atgagcaaat ggcagactat aacgcgctgg ggatcaccgt gcgttatctt
    12541 gctttcccgc gccaggggct ggacagcgat gcagagaaag aaatgaaagc tatctggtgt
    12601 gcgaaagata aaaacaaagc gtttgatgat gtgatggcag gtaaaagcgt cgcaccagcc
    12661 agctgcgacg tggatattgc cgaccattac gcacttggcg tccagcttgg cgttagcggt
    12721 actccggcag ttgtgctgag caatggcaca cttgttccgg gttaccagcc gccgaaagag
    12781 atgaaagaat tcctcgacga acaccaaaaa atgaccagcg gtaaataatt cgcgtgaaac
    12841 aacagataca acttcgtcgc cgtgcagtcg atgaaacggc agacttgccc gctgaattgc
    12901 ctcccttgct gcgccgttta tacgccagcc ggggcgtgcg cagtgcgcaa gaactggaac
    12961 gcagtgttaa agggatgctg ccctggcagc aactgagcgg cgtcgaaaag gccgttgaga
    13021 tcctttacaa cgcctttcgc gaaggaacgc ggattattgt ggtcggtgat ttcgacgccg
    13081 acggtgccac cagcacggct ctaagcgtgc tggcgatgcg ctcgcttggt tgcagcaata
    13141 tcgactacct ggttccaaac cgtttcgaag acggttacgg cttaagcccg gaagtggtcg
    13201 atcaggccca tgcccgtggt gcgcagttaa ttgtcacggt ggataacggt atttcctccc
    13261 atgcgggggt tgaacacgct cgctcgttgg gcattccggt tattgttacc gatcaccatt
    13321 tgccgggcga aacattaccc gcggcggaag cgatcattaa ccctaacttg cgcgactgta
    13381 atttcccgtc gaaatcactg gcaggcgtgg gtgtggcgtt ttatctgatg ctggcgctgc
    13441 gcaccttttt gcgcgatcag ggctggtttg atgagcgcgg catcgcaatt cctaacctgg
    13501 cagaactgct ggatctggta gcgctgggga cagtggcgga cgtcgtaccg ctggacgcta
    13561 ataatcgcat tctgacctgg caggggatga gtcgcatccg tgccggaaag tgccgtccgg
    13621 ggattaaagc gctgcttgaa gtggcaaacc gtgatgcaca aaaactcgcc gccagcgatt
    13681 taggttttgc gctggggcca cgtctcaatg ctgccggacg actggacgat atgtccgtcg
    13741 gtgtggcgct cttgctgtgc gacaacatcg gcgaagcgcg cgtgctggca aatgaactcg
    13801 atgcgctaaa ccagacgcga aaagagatcg aacaaggaat gcaggttgaa gccctgaccc
    13861 tgtgcgagaa actggagcgc agccgtgaca cgctacccgg cgggctggca atgtatcacc
    13921 ccgaatggca tcagggggtt gtcggtattc tggcttcccg tattaaagag cgttttcacc
    13981 gtccggttat cgcctttgcg cccgcgggtg atggtacgct gaaaggttct ggtcgctcca
    14041 ttcaggggct gcatatgcgt gatgcgctgg agcgattaga cacactctac cctggcatga
    14101 tgctgaagtt tggcggtcat gcgatggcgg cgggtttgtc gctggaagcg gataaattcg
    14161 aactctttca acaacggttt ggcgaactgg ttactgagtg gctggaccct tcgctattgc
    14221 aaggcgaagt ggtgtcagac ggtccgttaa gcccggccga aatgaccatg gaagtggcgc
    14281 agctgctgcg cgatgctggc ccgtgggggc agatgttccc ggagccgctg tttgacggtc
    14341 atttccggct gctgcaacag cggctggtgg gcgaacgtca tttgaaagtc atggtcgaac
    14401 cggtcggcgg cggtccgctg ctggatggta ttgcttttaa tgtcgatacc gccctctggc
    14461 cggataacgg cgtgcgcgaa gtgcaactgg cttacaagct cgatatcaac gagtttcgcg
    14521 gcaaccgcag cctgcaaatt atcatcgaca atatctggcc aatttagcgt catcttctct
    14581 ataaaaaaga gcgtggattg ggtacaatcc cgctcttatc accgcatttt gactagctca
    14641 ataaaagaaa tcagaccatg tttgaaatta atccggtaaa taatcgcatt caggacctca
    14701 cggaacgctc cgacgttctt agggggtatc tttgactacg acgccaagaa agagcgtctg
    14761 gaagaagtaa acgccgagct ggaacagccg gatgtctgga atgaacccga acgcgcacag
    14821 gcgctgggta aagagcgttc ctccctcgaa gccgttgtcg acaccctcga ccaaatgaaa
    14881 caggggctgg aagatgtttc tggtctgctg gaactggctg tagaagctga cgacgaagaa
    14941 acctttaacg aagccgttgc tgaactcgac gccctggaag aaaaactggc gcagcttgag
    15001 ttccgccgta tgttctctgg cgaatatgac agcgccgact gctacctcga tattcaggcg
    15061 gggtctggcg gtacggaagc acaggactgg gcgagcatgc ttgagcgtat gtatctgcgc
    15121 tgggcagaat cgcgtggttt caaaactgaa atcatcgaag agtcggaagg tgaagtggcg
    15181 ggtattaaat ccgtgacgat caaaatctcc ggcgattacg cttacggctg gctgcgtaca
    15241 gaaactggcg ttcaccgcct ggtgcgtaag agcccgtttg actccggcgg tcgtcgccac
    15301 acgtcgttca gctctgcgtt tgtttatccg gaagttgatg atgatattga tatcgaaatc
    15361 aacccggcgg atctgcgcat tgacgtttat cgcgcgtccg gcgcgggcgg tcagcacgtt
    15421 aaccgtaccg aatctgcggt gcgtattacc cacatcccga ccgggatcgt gacccagtgc
    15481 cagaacgacc gttcccagca caagaacaaa gatcaggcca tgaagcagat gaaagcgaag
    15541 ctttatgaac tggagatgca gaagaaaaat gccgagaaac aggcgatgga agataacaaa
    15601 tctgacatcg gctggggcag ccagattcgt tcttatgtcc ttgatgactc ccgcattaaa
    15661 gatctgcgta ccggggtaga aactcgcaac acgcaggccg tgctggacgg cagcctggat
    15721 caatttatcg aagcaagttt gaaagcaggg ttatgaggaa ccaacatgtc tgaacaacac
    15781 gcacagggcg ctgacgcggt agtcgatctt aacaatgaac tgaaaacgcg tcgtgagaag
    15841 ctggcgaacc tgcgcgagca ggggattgcc ttcccgaacg atttccgtcg cgatcatacc
    15901 tctgaccaat tgcacgcaga attcgacggt aaagagaacg aagaactgga agcgctgaac
    15961 atcgaagtcg ccgttgctgg ccgcatgatg acccgccgta ttatgggtaa agcgtctttc
    16021 gttaccctgc aggatgtggg tggccgcatt cagctgtacg ttgcccgtga cgatctgccg
    16081 gaaggcgttt acaacgagca gttcaaaaaa tgggacctcg gcgacatcct cggcgcgaaa
    16141 ggtaaactgt tcaaaaccaa aaccggcgaa ctgtctatcc actgtaccga gctgcgtctg
    16201 ctgaccaaag cactgcgtcc gctgccggat aaattccacg gcttgcagga tcaggaagcg
    16261 cgctatcgtc agcgttatct ggatctcatc tctaacgatg aatcccgtaa cacctttaaa
    16321 gtacgctcgc agatcctctc tggtattcgc cagttcatgg tgaaccgcgg ctttatggaa
    16381 gttgaaacgc cgatgatgca ggtgatccca ggcggtgccg ctgcgcgtcc gtttatcacc
    16441 caccataacg cgctggatct cgacatgtac ctgcgtatcg cgccggaact gtacctcaag
    16501 cgtctggtgg tcggtggctt cgagcgtgta ttcgaaatca accgtaactt ccgtaacgaa
    16561 ggtatttccg tacgtcataa cccagagttc accatgatgg aactctacat ggcttacgca
    16621 gattacaaag atctgatcga gctgaccgaa tcgctgttcc gtactctggc acaggatatt
    16681 ctcggtaaga cggaagtgac ctacggcgac gtgacgctgg acttcggtaa accgttcgaa
    16741 aaactgacca tgcgtgaagc gatcaagaaa tatcgcccgg aaaccgacat ggcggatctg
    16801 gataacttcg actctgcgaa agcgattgct gaatctatcg gtatccacgt tgagaagagc
    16861 tggggtctgg gccgtatcgt taccgagatc ttcgaagaag tggcagaagc acatctgatt
    16921 cagccgacct tcattactga atatccggca gaagtttctc cgctggcgcg tcgtaacgac
    16981 attaacccgg aaatcacaga ccgctttgag ttcttcatcg gtggtcgtga aatcggtaac
    17041 ggctttagcg agctgaatga cgcggaagat caggcgcaac gcttcctgga tcaggttgcc
    17101 gcgaaagacg caggtgacga cgaagcgatg ttctacgatg aagattacgt taccgcactg
    17161 gaacatggct taccgccgac agcaggtctg gggattggca tcgaccgtat ggtaatgctg
    17221 ttcaccaaca gccataccat ccgcgacgtt attctgttcc cggcgatgcg tccggtaaaa
    17281 taagcattac gttatgctca caaccccggc aaatgtcggg gttttttaat tgtgctgcgc
    17341 gaaagcagac aacaattttc ttgcttcact attggctgcc tgcatcacca tccacggact
    17401 gaacgcccac ggtgtggcat caataccgtg taatacatct gctaaatcac accattgata
    17461 atccatcact tcatcatcgt tgatctgtaa cgcactggtg gtgcgtgcgg caaataccgg
    17521 acacacttca ttttccacaa tgccattcgg atcggtggcg cgatagcgaa agtcaggata
    17581 gatagattca ggaggcgtaa tttccacgcc aagctcataa cggcaacggc ggatcaccgc
    17641 gtcttcgttg ctttctccca gttgtgggtg cccacaaacc gagttagtcc acacgccagg
    17701 ccacgctttt ttgctcagtg cgcggcgggt aactaataat tgtcctttgg cattaaacag
    17761 ccaactggag aacgcgagat gtaagcgggt gtctgccgtg tgtgcggcat acttttccag
    17821 cgtacccgtg ggaactccct gtgcattcaa taaaatgacg tgttccgttt gcataatttc
    17881 tcacatgtaa ttctgatcac tcatcattat agataattga agagtgaaat gattaaccaa
    17941 gtgagcgttt atcgacaacc gcccgttttg agcggatgcc gacaggtaaa aaccatttaa
    18001 aaaagtagca tcatatattt gaagcgactt tcaatggcca ttgcaacaca gtcagaaatg
    18061 acaggaatag agagagtaaa aagcaacagc agactgaata taacgcgcat taaaaaattc
    18121 atattattct ccatgctcat ttttcttcag cttgcgcatc agaagccaca tacgtactgt
    18181 cctgactttt aaatctttat cagagacgga ttccttcacc aggtcagcat gatgctggta
    18241 gccattaaag aagacattca gcacaacggc gctaagggtt gcgagcataa tgccgctatg
    18301 cagcaacggt tgcagtacgg ccggtaactt agaaaagaaa tcgtgagaga gcgtcggagt
    18361 catgccaacg ccgagactga tcgccacaat atagaggttg taacggttgg tggtgtagtt
    18421 acagcgcgac agaattcgaa tccctgtcgc cagtaccatg ccgaacatca ccagcccagc
    18481 accgcccagc acaaattgcg gaatggaggc gaccagcacc gccatttttg gcaccatgcc
    18541 aaacaggatt aatataattc ccgaagaaat acacacccag cggctataaa tcctttcgca
    18601 tagcgagtaa tgagcaagat aaaaattaag acagcaaagg agatacctaa ataaacggga
    18661 ttaccatatt gcggattccc tttacctccg gcggcccagt caatacccac ctgaatgatg
    18721 ctaagcccaa tagaagtaat aaccacaccg gtaaccagtg gcgggaataa aggcatcaag
    18781 cgaccaataa atggcgctaa taatgtggtg ataaaacccg cggcgatagt ggcaccaaat
    18841 atccccagca ggccgatatc cgggttcatc ccaatggcta tcattggtgt tacagcagca
    18901 aaggtcaccg acataatcac cggcaggcgg atccccataa agcggccgat accgatacgt
    18961 tgcaataatg tgacgatccc gcagcaaaag agatccgagc taatgagcat cgcaatagca
    19021 tcttttgaga ggcccagtcg gtcaccaatc ataagaggaa cagcgactgc acctgcgtac
    19081 atgacaagga cgtgttgcag accgagtatg atcagctttc ctggtgataa tatgcggtca
    19141 acctcatcag ttgggcggtc ttgcccagaa gatgagggaa gttgggaatc tatggcgctc
    19201 atcagtgttt ctcctttcgc ccgccagagt aaaaagcaac cgtttacgtt aatgaaactg
    19261 tctaaacaca tgctcgtgga gtgccagttt ttgcgaggaa ggggagttag agacaggaat
    19321 tgcggaggca atcacataat cgtgcttaaa ccacgaaaat gacaagagaa cctgacaatg
    19381 gaattatctg taattttctc atattgatat ttcattaatc attctggcat gtcgcactct
    19441 agcatttaat cgtttttatc tggatagcgc tcttttgatc ggcaatgttg aaataatgaa
    19501 agcggcatag attgaggggc aataatttga actcagtcgc aaaattataa ttaaaacggt
    19561 catgctacct aatatatgcc cgtttattgc atcgcgaaat ttgagaagac ataactcatt
    19621 aaatgaaagg gatgcaaatg aataagttta tcgctgctga agctgcggaa tgtataggct
    19681 gtcatgcttg tgaaattgcc tgtgcggtgg cacacaatca agaaaactgg ccgctgagtc
    19741 acagtgactt tcgaccgcgt atccacgttg tagggaaagg ccaggctgcg aatccggtgg
    19801 cctgccatca ctgcaacaat gccccttgtg ttacggcttg tccggttaat gctctgactt
    19861 tccagtccga tagcgtacaa ctggacgagc aaaaatgtat tggctgtaaa agatgcgcaa
    19921 tcgcttgccc ctttggcgtc gttgagatgg tcgatacgat tgcgcagaaa tgcgaccttt
    19981 gtaaccagcg cagttccggc acgcaagcct gtattgaagt ctgcccaaca caggcgttac
    20041 gactgatgga cgataaaggg ttacagtgga taaaggtggc tcgccagcgc aaaacggcag
    20101 caggaaaagc gtcatcagac gctcagccat ctcgcagtgc agcgttgctc cccgttaact
    20161 cgcgtaaagg cgcagataaa atttcagcga gtgaacggaa aacccacttt ggcgaaatct
    20221 attgcgggct ggatccactg caagcgactt atgagagtga ccgctgtgtt tattgtgccg
    20281 aaaaagctaa ctgcaactgg cattgcccgc tgcataacgc tattccggat tacatccgtc
    20341 tggtacagga aggaaagatt attgaagcgg cagaactttg ccaccagacc agttccttac
    20401 cagaaatctg cggcagggta tgcccacagg accgtctttg tgaaggcgca tgtactttga
    20461 aagatcactc tggcgcagtc tctatcggta atctggaacg ctacatcacc gataccgcgc
    20521 tggcgatggg ctggcgtccc gatgtcagca aagttgttcc ccgtatcgaa aaagtggcgg
    20581 tgattggcgc tgggccagca gggttagggt gtgctgatat tctggcgcgc gcgggagttc
    20641 aggttgatgt ctttgatcgc catccagaaa ttggcggtat gctgactttt ggcattcctc
    20701 ctttcaaact cgataaaacg gtattaagcc agcggcgaga gatattcacc gcaatgggaa
    20761 tcgactttca tcttaactgt gaaattggcc gcgatattac ctttagcgat ttaacttctg
    20821 aatatgatgc agttttcatc ggcgtgggga cttacgggat gatgcgagca gatctgccgc
    20881 atgaagatgc gcccggtgtc attcaggctc taccgttcct gactgcccat acccgccagc
    20941 tcatgggatt gccggagtct gaagagtatc cgctgacgga cgtggaaggt aagcgagtcg
    21001 tggtattggg cggtggcgat acgacaatgg attgtttgcg gacttccatt cgcctcaatg
    21061 ctgccagcgt gacctgcgcg tatcgtcgtg atgaagtcag tatgccgggc tcgcgcaaag
    21121 aggtggttaa tgcgcgcgag gaaggtgtcg agtttcagtt caatgttcaa ccgcaatata
    21181 tcgcttgtga cgaagatggg cgtttaactg cggtgggcct gattcgtacc gccatgggtg
    21241 agccggggcc ggatggtcgc cgtcgtcctc gtccggtagc gggttcagag tttgaattgc
    21301 ccgccgatgt tctgattatg gcctttggtt tccaggcgca tgccatgccg tggttgcagg
    21361 gcagcggaat taaactcgat aaatggggcc tgattcaaac cggtgacgtc gggtatttac
    21421 ctacccagac gcatctgaaa aaagtctttg ctggtggtga tgcagttcat ggcgcggatc
    21481 tggttgtcac tgcaatggcc gcaggaaggc aggcggcgcg cgatatgtta actctgtttg
    21541 atacgaaggc atcgtgatga aatcgttaat tatcgttaat ccggctgact gtattggctg
    21601 ccgtacttgt gaagtagcct gtgtggtcgc tcacccttca gaacaggagt tgaatgccga
    21661 tgtcttttta ccccggctga aggtacagcg actggacagc atcagtgcgc cggtgatgtg
    21721 ccatcagtgt gaaaacgccc cttgtgttgg cgcttgcccc gtgggggcgc tgacgatggg
    21781 cgagcaggtg gtgcagacca attctgcccg ttgtattggc tgtcagagtt gcgtcagtgc
    21841 gtgcccgttt ggaatgatca ccattcagtc gttgccgggg gatacccgac aacaaattgt
    21901 gaaatgcgat ctctgtgaac agcgggaaga ggggccagcc tgtgttgaat cttgcccgac
    21961 acaggcgctg cagttgctta ctgaaagaga actcaggcga gtccgccagc agcgtattgt
    22021 tgccagcggt gagaatcctc tgtgaaagaa atctccgcgc tgaaaggcgc ggagaatcta
    22081 ttagattgcc caaccacccg cgtaaaatgc gaccagtgcg acaggaacgc taatttgttg
    22141 cccgagtacc agactgaatg cggtaaatgc cgtcaaggaa atcgcacaac caatcgccat
    22201 tggcaaattg gcctacaacc ccatcagcaa cgagccgaag cccgcgacca gacaggtggc
    22261 aacaaacaca gctgcgggag gaaaacctgc tttgcccagc attcccggaa cgacgataac
    22321 ggaataaacc atggccagaa aggtcgttaa gccagccagt acttcctgac gaacggtact
    22381 gccacgagcg gtaattttaa aataattatc cagcgcgccc tgtggctttg gtgcgtccgg
    22441 tgtttgtagg atgtctccag acataatgat gtcctcacga ggatgttaaa gttttattat
    22501 tagttgcgtt cgtacaccag acgaccatca acgtaggtgc ggtagatcga acggtcatcg
    22561 cccaacgtca tcatcacgaa caatttgtcg actaaagaaa cagagttgtc atagcgcagc
    22621 tgctgtagcg gagtggcggt gggttccatc accacgaaat cagcctcttt gccaggtaaa
    22681 aagttgccaa tcaaatcgtc aaggcccaga gatttcgctc cgccgagcgt ggccaggtaa
    22741 aacgcttcat atgccgagag gcgatagcct tgtaattgca acactttgta ggcttcgttc
    22801 agcgtttgca gcatgttgaa agttgttccg gcaccgatat ccgttcccat gcccactttg
    22861 actttcttct gcccagatgc cgcatgcatc gtgaattgcg gtagcaatcg ccggagctgc
    22921 gccatttaca ccgatttccg agatcgattt cgccccgaac gggccgactt tatcgtcgct
    22981 cggcaccagc acagcgcgga aatcacgcgg aatgtcacca attttcggtg cgccgtaact
    23041 gcgtaaatca cgcgttaacg ggtgaccttc ggcgtcataa atgatctctt cgctcatact
    23101 gtggccgata gctcgcaggg tagcaccgta gatttgtccc aatgctaatt ccgggttgac
    23161 cggtgtaccg cagtccagca aggcgtagaa tttatccagg cggatttcac ccgtacgcgt
    23221 gttgacggca acttcagcga agtttgcgcc atacgggaag gcgaaatcag gcgtgatata
    23281 actgccagtt cccaccagtg aaccaaagcc gatgccggtt tcgcctttat gggcaatatc
    23341 cccgaaacta acttcgcctt tcttgccgcg cacgacgccc ggcgttgcca gttgaacatc
    23401 tgccactgcc tcacctaaca tttgcgcgcc gtggaacaga attttctccc gtagattttc
    23461 cgctgccaaa cgcgccgcgt tacccgagaa gcaagtaccg gacgaggcat atgcgccttt
    23521 atcaaacaac gcatgatcgg tatcaccgga gataacatgc acgtcctgcg gtgggcagtg
    23581 cagcacttct gctgccagtt tcgtcactac agtatccaga ccagtaccaa tatccgcacc
    23641 gccagaatga acgataaagg tgccgtccga ttccagtttg atcatgcagt tagcctgatc
    23701 gatatccggg atacccgatt tctgcatgat aatggcgacg ccgcgaccga tgtgccagtc
    23761 accattttgc ggtttcggtg acgaccattg gatcatctcg cgaccctgac gcaggatctc
    23821 ttccagtgca cagctggcgg cggaaggaac ggaggtcggc gctttacctt caccgattgc
    23881 accgagaatt ttcagctctt gcccttcgtg tacccggtta cgttcgataa tttccagttg
    23941 gtcgatctgt aactgttcag ccagttccgc taatgccatg gtgatagcga agttaccttt
    24001 cggtgcgcca taaccctgat aagcaccatt tgggcaaatg ttgctgtagt aggtggtgac
    24061 ctggaaatcg acgttatcgc acggatataa cggcagcgac agcgccggtc cgttacacgg
    24121 tacggtgagt gagtggttgc cgtaagggcc agtgttggcg cggaaatcca tcttcactgc
    24181 cgtcaggcga ccatcttttt tcgctcccag tttgacggtg actttcgcga cgtgacgaga
    24241 ggtgttagca ataaactctt cttcacgggt gtagcggaat agtaccggac gcccggtcac
    24301 gcaggttgcc caggcgcaca cttcttccag caggatgtcc tgtttggaac caaaaccgcc
    24361 gccaactcgc tctttaatga catgaacttt atgctgtttc atgcccacga ggcgcgcgac
    24421 ctggcggcgt aagtgccatg gtacctgggt ggaggcgtgg ataaccagac gatcgccgtc
    24481 catccgggta aagcagatat gtgtttcagt cgggcactgc tgtgcctgcg ttgagttata
    24541 ggttcgctca atgatcacat cggcatcggc aaagcctttg tccatatcgc caatatgacc
    24601 atgaatactg gcggcgatat ttttgcgagg gcgagaaccg atcgggaagt tgatgatcat
    24661 atgctcgccg cgctgggctg catggctgtt atcgtcttcc agagtatctg gcgcaccagc
    24721 aacatacacc accggttcat cgtgcacgac aggcgcatct tccgccattg cttcgtcgat
    24781 cgacattacc ggcttaagca cttcatattc aacgtcgatg agcttcagtg cttcgagcgc
    24841 aatttcttca ctttctgcga cgaccgcagc aacacgatcg ccgacgtgac gcattttctt
    24901 gccgaacata cggcggtcaa gcggtgacgg ttccggtgcg ctctgacccc ccggggtata
    24961 gtagatatca gggcaattca ggtgagtaat aacgtgaacg acgcccggta aggcttcagc
    25021 tttgctgaca tccagatgag taatcagtgc gtgagcgtgt gggctacgta acattttaat
    25081 gacgcaggcg tcagccgtta cgcagtcttc aacatagcag ggtttcgcct gcaccatttt
    25141 cgcggcatca gttttaggat aatgcttgcc aatgacgtca aggtcgtcac ggaaagtcgg
    25201 agcgatatca atggtggcct gcggattatt tttacgtgca accgccagtt caatgacctg
    25261 atagtattgt tgccagcctg catcgcggct gaacaaacca gaaagcgcgt cgtcgatctc
    25321 ttcgcgggta ggtgcggcga tgcgatcgag aagatcggtg ataatcagag ctgcagctgg
    25381 atcgttataa ccagactgca ccacgccaac atcaaccatt gcctgttgaa ccagacttaa
    25441 ctcgttccat ttacccagag attctgcggt acgaatatct gccttctcta actgtgcggc
    25501 aataagcaag gacgcgttaa cgatattacc gttaaagatt attgcgtcag aaccggcaaa
    25561 cccgaagcca tcatcactgt tgcgtacaga gtgcattccc atgttaaaca acagcttttg
    25621 cacgttttcg cctggattaa cggttagctc ctgaggcgcg ccatttaaag taaagtggat
    25681 gatcatacgg cttcctcccc agcctgttgg cagtcggcat acaggtcggc taccagaact
    25741 cccgtgatat agcgtttata ggccacgctg ccccgcaaat cttcctgcgg gaaaatggcg
    25801 ttggcgacag cctgttcaag tgcattgcct tccagatttt gtttttcgac atcgtgcaga
    25861 cgcagtgctt tactggcgac gccatccagc gcaattcgca taccgtcgtg gtctgtcatt
    25921 gcaacggctg ccgtcacgac ggttaaacct gcctgagaac ggctaatttt gcgggtcgca
    25981 caggtgcgat acggatcttt aatgataatt tcggttagca ggcgatcgca tgggcaggcc
    26041 aggtagtcct cgattgacag cgtttcgccg ttgccaaaaa ccagttcagc atccagtgct
    26101 agcaggacgg gaagcagcac tgactcttcc tgccgggcgg cgatttcacc accaatggtc
    26161 gactgattac gaacatggcg tgagtaaaca aaaccgaggg cttcacgcag tgctgcagga
    26221 ataaagcgcg catcacgcag tggctgcaag cgagacattg caccaatccg cagtgcaccg
    26281 ttatcccagt caacccagtc cagttccaga tcctgtaagg aaatggcaat ctttttatcg
    26341 gtacgggttg gtgtagcatt gagtttgctg cccccggcga accagacggc ttcatcctgg
    26401 tagcggcgct tcagttccag cgcctgttcg acagagtcgg gcctgaaaaa ttgttcaatc
    26461 atcttctttc cttatttaac cctcttccct gcgggaagag ggcatctgtc atttatgcca
    26521 gcgcatccat ccgacgccac atactggcag cggcttttct ggcttgtgca taaatggaat
    26581 cgcaatcgaa gttaaactga cggtcttcat agaccatcac accattgacc atcacgctgt
    26641 gaacgctgcc tgagcccata ccgaaagcga tatgcccggc gatattgtct gccagcagcg
    26701 gcgtcggcga gttgtaatcg caaatggtca aatcagcttt gtaaccggct tccagaagcc
    26761 caaatttcgc gccaaagttg cggctcatca gttcgttacc gttagtcagg gctttggcaa
    26821 aactgtcagg ccacagcgga ccacccgcat cgcgatgttt aaagaaggca aatttcatct
    26881 cttcaaacat gtccgaacca atgccgtccg ttcccaatgc caggttgcgg atgtcgctaa
    26941 ggtgatggtt gtagccgaca tggttgttca tgtttgaacg ggcgttatgc accaggaacg
    27001 catcgcgctg attgagtagg gcgatgtcat ctttcgacaa gtacagccca tgagcgacca
    27061 gcgttttgct gtcgatgagg tcgaattgcg ccagtcgtgc cagcaggtct ttgccgtacc
    27121 agtggtgact gtaggaaacg tcgtaaaggt cttccgcagc gtgaatatgc aaaccacggc
    27181 ctgtggcttt cacggcttca cgcagcatct ccagaccggc atccggcacg gtaaacggcg
    27241 cgtgagcacc gatatgtgct tccaccagat acggctcgct tgtcgctttc ttcgcctcat
    27301 caatcaaacg ggcgaaacgg atgttttctt ctacaccttc ctgcaactct ttgatgccgt
    27361 tgttacggtc agtagtttca aaacaggtca tcgcgcgcag gccaactttt aaaaatgcgt
    27421 cgcgcaatgt ggagagcgac ccgccgatat acgccggcga ggcatggtga tcgataaccg
    27481 atgtacatcc gctcttaatc gcttccaggg aacaaatcag tccgctgtaa tagagcgact
    27541 cttcatcaag ggcgcgatcg agccgccacc agagattttt cagcgttgag atgaaatccg
    27601 ggcaaggggc gatgtttgcc ataattccgc gggaaagctc cgagtaaaaa tggttgtgcg
    27661 agcagacaat tcccggcatc acaatccgac catgcatctc tttgtagctg gcatcggggt
    27721 agcgttgcgt cagggcatcg ccgatagcga caatcacgtc attttcgata gcgatatcaa
    27781 cgccttcctg cactttcgcc gggtgtaact gcactgcggt gacattcttc agaatcaaca
    27841 tgattacacc tccacgcggc ccagcagata atgatgatgc tggtggacat ggctgatgat
    27901 gcggcacata tcgttcagct ccggtggtac gttgttaaac tgaccttcgc tgtcgatgtt
    27961 taacacccag ctttggttat tcagacgtac tcgtacccgg caatcttcca caaggaagcc
    28021 tggggtgctg ctgttatcaa agtcttgcgc caggctgaag acggtgattt tgtcttcgta
    28081 tggtttaccg ttccacggac agaactgagc gcagttgccg cattcgttac agtaagcgtc
    28141 gaggtgcagc gtctggaatc tattctggaa gccagggact gcaatggaga cgttggcgcg
    28201 gttcgggcag acatccacac acttgttgca aacgtagtta cattcgaggc agcgagcggc
    28261 ttcctgggcg acaaacgcgt cacgatcgtc actgttcacc agcgtgatag agatatcgcc
    28321 tttacgttga tagatttccg ctggattgac gttgttccag tatttatcgt tctggtggga
    28381 acggatattt tcccgactaa ggatggcatc ggtcgcccga cgcgcggttc cgacagcagc
    28441 gacaatggag gatggtccgc gctgtacgtc gccgatcata aagacgtcag tcagacgagt
    28501 ttcgccatta tggtcgacgt ctggccagcc gtttttgtcc agcggcacgc ccatcgcatt
    28561 cagggcttca gtatcctgct gttcaccaat ggcggtgatc aggctgtcta catgcagtgt
    28621 tactgtttcg ttggtttcaa ccggacggcg acgacctttc tcatccggtt cgccaagcga
    28681 cataacgcgc aaggttaagg tgccatcagc atcgaaacgt tccggattat tcaggaaacg
    28741 gaactctacg ccgtcgtgca acgcttcttc atactcttcg cgccatgcgg gcatctcttg
    28801 tagtgaacga cggtaaacga tcgttgcttt ttctacgcct ggaacgcgta acgccgcacg
    28861 agcgcagtcc attgcggtgt tacccgcccc gacaacgacc acatgtttgc ccagcttgag
    28921 cgctgtaccc ttgttgtatt cacgcaggaa ggggagtgat ttccagacat tttggttgtc
    28981 gcccgccagt ttcacaccgc tatttttatc agtgccggtg gcaatcagaa catagtggaa
    29041 gccctgattt tttaactgtt caacggttaa atcgggtgag cagccatact caaatttcac
    29101 gccgtgagcg gcaacaaaat cgatatcgtg ctgaattaac tctgcaggaa tacggaactg
    29161 aggaatgata tttttcacca cgccgccagc attggcttcg cgttcaaaca gcgtaaccgg
    29221 atggcccgct ctggcaagga agtaacctgc tgccagaccc gccggacctg caccaatcac
    29281 ggcaaccgga tggcgtgaac cagaaccggc gggtttgtgc cagcgttgct tatattcatc
    29341 ccaacctttt tccagcgcga cttttttcag ttcgcggata ttcagcgcac tgtcgtaatc
    29401 caggcgggta cagttgtatt ggcactggtg atcgcaaata tgaccggtaa tggcaggtag
    29461 ggcgttgcgt tggtagatga gttccagcgc gtcggcatag cggtgttcgc caagcagacg
    29521 gatgtattcc ggaatatctt gcttgatagc gcaggcagta acacaggggg caacgtagca
    29581 atcggtcagc ggcaggtctt ctgccacttc aatacgctct tctggcttcc agtgtttctg
    29641 ggtgtattcc atggttaacg cgtctgctgc cagtctgttc agtcgttcga cgtcaacatg
    29701 gtcaagtccc caggcgtcgg agccttccag ctcgcgcatg caggcactta agcgcagata
    29761 gccgccaggt ttcagcaggt cggttgccat agtaataggg cgaatacctg tatcaaaaat
    29821 atcgcggata gtcagctgac tggcaccacc ggaataagaa atgggcagtt tgccgtcaaa
    29881 ggcgcgagag agaactgctg caacattgat ggagagcggg aacagcgcac ggcctgacat
    29941 atacatctct tcaccaggca gtgcgccttt attgttgatg gtgccgagag tgttagtcag
    30001 ttttacgcca aagccgagtg atttttcttt tgccagtacc atcaggcgct ccagcatttc
    30061 cagtgcttgc gtcagcttga ggtcgtgatc aaatgactct tcttttaagc ctatgtaacc
    30121 gaaaccgcag acatcgagga tctcacgaac acgcgcgtac cccagtaagg tcgggttaag
    30181 tttgacaaag gtgttgagcc ctttttcttc cagcatgtag cggcaaatgg cttcgatttc
    30241 atgcggagga cagccgtgca tggtggagag ggtgacgccc tgcaccatac tggtggggat
    30301 gcgagcgggt aaggcttgca agttttcgcg tttttcctgc aatccgtggc gggctaaaaa
    30361 tgcgtcatcc tggagtaatt tattcagcgt atcgcgatat tgagcgaatt tcggatggtc
    30421 agatgcgtcc atcatattgt cgatgaactg ttgcatcggc ggttgcttaa taccttcgag
    30481 gttgtaaccg acgctcatat taaagatgaa cgatttaccg gaatcagaag gctgtaacat
    30541 cgcttcgaga aggtgcaggg caaaccaggc tttgaggtat tcatcccagg ctttaagcaa
    30601 ggtaaattcg gtagaccatt cggtgttaaa gcattcgtct tcggcatcaa tacagggctt
    30661 ttccagctcc aggcggtcaa gaatttggac ggtttttagt tcgatgaagc gtccgccagt
    30721 cagccaggaa gtgacaatat tttgcgcgag ctgcgtgtgc gggccagcgg cagggccgac
    30781 gggagtggca caggtttcac cgaagacgct aacagtttta ccttttacag gtgagtaaaa
    30841 ctgttgctcg ggaataccaa agattgagcg ttgttgttgg tattcatcaa atatgcgcgt
    30901 caaaagttcc tcaaacggaa tgggacgcat aatatccccc ataaccctct ccttagctta
    30961 tacagatatc attgagtgac cggtgttgag aaatccggtg gtttaggggg atagagcaac
    31021 aatcgcacca ctcttgccag gtttggcggg aaatataaaa aaatgtgagg tggctcgaaa
    31081 cttaaaggct ttttgtgaat gaactcacag gtgccaaatg agaattaaaa tcactcctga
    31141 aaattattat tgtcagccca tttatcactt tttctctttt ctcaaaatca gtgattcctt
    31201 atcattatga taaagagtgc tggagagatt aaatatttga acgcaatcgt ttaactacat
    31261 tttctaaatt ttagtatacc ttttttgcgc tgtaataaag tcgtccggag tatcaatatc
    31321 taaaattatt tctgcatttt ctatttcgac ggaataatga tctcccataa ggagtgcctg
    31381 acgcatattg gtgacattgg gtcgctggat tgcctgcatc aggcatggtt ttgacactaa
    31441 aatcggatgg ccggggatgc cattatggag tggcagtatt gcgccatcat ttcgtaacga
    31501 ccagattttt ctaaaaatat cgatggtgag ggttggcatg tcaccgtggg tgagaaaaca
    31561 atgttctgtt tgcactgcgg gtactgcggc cttgacggat gttagtaaac cctgcgcata
    31621 atctgggttg tgaataatag tgatattgct ctggttcgca taacgttcgt gcagttcatt
    31681 accacgatag ccggtgacta aaataattcg gctacaaaac tgcaacgcat ttttgatact
    31741 tgtatcaaga attgttccct gttcccaggg gaacatcatt ttccattgcc ccattcttga
    31801 tgataatccg gcagcggtaa ttatacagtc gatggctgac atagtggttc cattatttat
    31861 aggggctaat aacgcctgat aattgcgtta cttcctgatg gattaagttg gggataattt
    31921 atcccagaga ggtcataaag actcgtgaaa agtatagttg acccatcggc attagtcatt
    31981 gatttaggtg cgcagaaacg tcccactgtg atttctgttg ttggtgctgg ggggaaaacc
    32041 agcttgcttt tttggctggc agaactgctc caggctagcg gcaggcgtgt gttaatcacg
    32101 acgacgacac atatgtttat gccaacgtct cactggcccg tggttttctg tcgtgatccc
    32161 gccatgcttc ctcatgcgtc tcttacatcc cccatttcat tttgttttca cagctggaaa
    32221 gcgaaccagg gaaaagtgca gggatttacg ccagaagcga ttgatgcact ggtgcaacga
    32281 ccagagtgtg acgtaattct cattgaagca gatggctcgc gtggaatgcc gttaaaagcg
    32341 cctgatgagc acgaaccttg catacccaaa agcagttgct gcgtgattgc tgtgatggga
    32401 ggacatactt tgggcgcgaa agtgagcaca gaaaatgtcc atcgctggtc gcagtttgct
    32461 gatattaccg ggttaacacc tgatgcaacc ttgcaactga gcgatctcgt tgcgctggtt
    32521 cgccaccctc agggggcgtt taaaaacgta ccgcaaggtt gtcggcgagt ctggttcatt
    32581 aaccgttttt ctcaatgtga gaatgcgatt gcacaaagcg agctccttca accgctgcaa
    32641 caacacgacg tagaggcaat ctggctgggc gatatacaag aacatcctgc aatcgcgcgc
    32701 agatttgtga attagcggcc cgggtattcc gggttactga aaggatatcc attgaggttg
    32761 gtatgaatat tttcacagag gctgcaaaac tcgaagagca aaattgtccg tttgcgatgg
    32821 cgcaaattgt tgatagccga ggctcgactc cccgccattc tgcacaaatg ttagtgcgcg
    32881 ccgatggttc tatcgtcggt acaattggtg gcggaatggt tgagtggaag gtgattgaag
    32941 agtcacttca ggcattgcag gaacgtaagc cgcgattatt ccatggacgt atggctcgta
    33001 acggtgcgga tgctgtcggg tcagattgtg gaggtgcaat gtcagtattt atcagcgtcc
    33061 atggtatgcg tccacgtctg gtgttgattg gcgcggggca tgtcaaccgg gcgatagccc
    33121 agagtgcggc gctattagga tttgatattg ccgttgccga tatttatcgc gaaagcctca
    33181 atcctgaact attcccacca tcaaccacgc ttctccatgc tgagtcgttt ggtgcggcag
    33241 tggaagaact ggatattcgc cctgataatt ttgtcctgat tgccacgaat aatcaggatc
    33301 gtgaagccct cgacaaactc attgaacagc ccattgcatg gttggggttg ctggcaagtc
    33361 gccgcaaggt tcagcttttc ctgcgtcaat tgcgtgagaa aggcgtggct gaagaacata
    33421 ttgcccgttt acatgcgccc gttggttaca acataggtgc ggaaacgccg caggagatcg
    33481 ccatcagcgt gctggcagaa atattacggg tgaaaaataa cgcgccgggt gggctgatga
    33541 tgaaaccttc tcatccttcc ggacaccagc tggtggtgat tcgcggtgcg ggagatatcg
    33601 ccagtggtgt ggcgctacgt ctgtatcatg cgggttttaa agtgatcatg ctggaagtgg
    33661 aaaaaccgac agtgattcgt tgtaccgtgg cgtttgccca ggccgtgttc gatggcgaaa
    33721 tgacggtcga aggcgtcact gctcgcctgg caaccagctc tgcggaagcg atgaaactta
    33781 ccgaacgcgg attcatccct gtgatggtag atcccgcctg ttcattgctt gatgaactga
    33841 aaccgctttg cgtggtggac gctattctgg cgaaacagaa tttgggaacg cgggcagata
    33901 tggcaccagt aacaatcgcg cttgggccgg gctttactgc agggaaggat tgtcatgcgg
    33961 taattgaaac aaatcgcggg cactggctcg gtcaggtgat ttactctggt tgtgcgcagg
    34021 agaataccga tgttcctggc aatattatgg ggcataccac ccgacgggtg atccgtgctc
    34081 ctgctgcagg cattatgcga tccaacgtga aattaggcga tctggtgaaa gagggcgatg
    34141 tgattgcctg gattggtgag catgaaatta aagcaccgtt gacggggatg gtgcgtggct
    34201 tgttgaacga cggcctggca gtggttggtg gttttaaaat tggtgatatc gatcctcgtg
    34261 gtgaaaccgc tgatttcacc agcgtttctg ataaagcccg ggcgattggc ggcggcgtac
    34321 ttgaggcgtt aatgatgttg atgcatcagg gcgtgaaagc gacaaaagaa gtgctggaag
    34381 tggcttaaat gaaaatggcc ggatggtatt acccacccgg ccttaaaatt actgacaaat
    34441 caccgttccg gttttgcctt caatcccttc tttcgctttg ctcaatacgg taatcagtgc
    34501 ttcacgacct gcgcgagagc gagcaaacga ggcggcagct tccactttag gcagcataga
    34561 acctttcgca aaatgacctt cttcgataaa gcgttcagca tcacttaatg acaggcgatc
    34621 gagccactgt tcattctctt taccgaagtt aatggccact ttttctaccg cagtcagaat
    34681 gatcagcata tcggcatcaa tcatttctgc taaacgggcg ctggcccagt ctttatcgat
    34741 aaccgcgctg gcaccacgca gatggttacc ttcacgaata acagggatac caccaccgcc
    34801 aacggcgatc accacctggc ctgcatctac cagagcttta accgtttctt tttcaatgat
    34861 atcaaccggt tttggcgagg caacaacacg gcgatagccg cgaccagcat cttctttcag
    34921 agtgtaaccc tgttttgtca gttgctctgc ttcttgctcg gtaaagaacg aaccgatcgg
    34981 cttggtcggg ttgaggaatg ccggatcgtt agcgtcgact tcgacctgag taaccagcgt
    35041 tgctacaggt ttattaatgc cccgagaaag cagttcttct cttaacgcgt tttgtagatc
    35101 gtaaccaata taaccctggc ttaaggcaac acagacagac atcggcagca ttggcgagtg
    35161 tgcttcagtt tttgcggcag cttcaaaggc ttggttaatc atgcccacct gcggcccgtt
    35221 accatgagtg acgataactt catgtccttg ggcaattaaa tcaacaattg cctgagaagt
    35281 aatttttacc gctttcattt gtccggccag gtcatcgccc agcgcattcc cgcccagggc
    35341 gagaacaatt ttcttactca tttttcctca ctcatcattc ttgattgtgt atagatccct
    35401 gacagtggtt agagcacggg agggacaaac ggtttgcgtc gcaggaaacg acctcgcccg
    35461 gctttgcctg taaaggtgcc gtcacagaaa atcgtttcac cacgggataa ggttctgaca
    35521 atcgcgccct gacaggtaaa accctcccat ggcgagtagt cggcgttgtc gtggagatgg
    35581 cgatgctgaa tttgttggct ctgacgtggg tcgataatca ccacgtcgcc atcggaaccg
    35641 ggcgctaata atcctttttg cggccacagg ccaaacagcc tggcgggcat tgcgctggtt
    35701 aattcaacaa agcgttccgg tgttatacgt cccgtcatca cgccactgga aaacagtaac
    35761 tgcatgcggt tttccacacc gggtaagcca tttgggcagc gactgaaatc gcctttagaa
    35821 atttgcaggc gttgagccat cgagaaggtg cagtgatcgg tcgccaccac gtcaatcgca
    35881 ccatcgctga tgccacacca cagtttgtcc tgctcgcgta cgttacgcag cggtgggcta
    35941 agaatgaact tcatgccatc ttctgtatcg taactgcgtt cgtccaacag gagatattgt
    36001 gggcaggttt caacccagac tggctggtga ttcgcacggg caagacgcag ataatccaga
    36061 cctaagccgt tagacaggtg cacgatatag agcggggcgt taccggcaat ttgtgccagg
    36121 ttaatcatgc gggcgatggc ttccgcttcg cattccagag ggcgactcaa ggcatgatag
    36181 cgcggcgcgg ttaaccctgc ggcgataaat tccgcccgct tgctggcgat agccgcatca
    36241 ttttccgggt gcacggtggt cagcgcgccg gattcatgca agcggcgtaa tgcctgcaaa
    36301 acctcgtcat cgttgagttt gtattgatag gttaaataga gtttaaaact gctcagtcct
    36361 tcctcgacca tcatcgggat ttcgtcgagg attgcgtgat taatgtgctg gatcacaccg
    36421 tgaaagctgt aatcgatgac cgctttatgg gcggcataac cacgataaac ctccagttga
    36481 tggcgtaacc gacagccgtt tgggccaaat cccatatggt caataatggt tgttgtaccg
    36541 ccacacgcag ctgcgcgggt accggtaaaa aaatcatcac aactgcgcgc gatgccgaca
    36601 tcaatattga aatgcgtatg gacatccacg ccgccaggga aaacgtaaca gccagtggca
    36661 tcaatttctt catacgggag ctgcggcgaa atattgttgc ccaactggcg aacaatcccg
    36721 ctttcaatca gcaaatcctg tttggcttgt ccatctgcgt taacgacagt gccgtttttg
    36781 atcaatacgc gcatagcaaa ctccaaaaac cctccggcga accggagggg aataagtgaa
    36841 gagtaattac tctgttgcca gccagcttaa cgggattgca gcgtacatcg cggcacaggt
    36901 caccaggtga gatttccagg ttttttcgtt aggtgcatgc gcttcaggtt ctttacccgg
    36961 gccaaagccg atcaccggaa tgccgtgacg gcccatgata gatacgccgt tagttgagaa
    37021 ggtccactta tcaacaaccg gcgctttgcc aaacagccct tcgtaggcat tcaccagtgc
    37081 tttaacggtg aagtgatctt cttccacttt ccaggtcggg aagtagcatt cggttgggta
    37141 aaccaggcca gtccaggacg gacggtcgta gttgtacata gaaacaaccg cgttagcttt
    37201 ctgtactgca ggcagggcgc ggatttcgtc cagcgcgcct tcccaggttt cgccccaggt
    37261 cagacggcgg tcaatagaga cggcgcagct atctgctaca gcgcaacggc ttggggatgt
    37321 gaagaagatt tcagaaacgg tgagggtgcc tttgccgagg aattcgtcat aacccagacg
    37381 ttgggagagt tcttgtaatt cgccaagaat cggacccatt ttgaaaatgg cgttgtcacc
    37441 gcgttctggc gcagaaccgt ggcagctaac accctgaaca tcaatacgaa tttccatacg
    37501 accgcgctga ccacggtata tctggcagtc ggttggttcg gtactgacca caaattccgg
    37561 gcgaatgccg gattgttcaa taatgtactg ccagcacaga ccgtcgcagt cttcttcctg
    37621 cacagtaccg gtaaccagca gggtatattc atcttccaga ccgaggtctt taatgatttt
    37681 accggcataa accatagatg ccatgccgcc ttcctggtcg gaagtaccgc gaccaccgat
    37741 tagctcatca gtttccatgc cttcgtacgg atcgaagtcc cagtttttga tgttgccaat
    37801 gccgacggta tcgatatgag cgtccattgc caccagacgc gggccgtggc cgatataacc
    37861 gagaacgttg cccatcgggt cgatttcaac tttatcgaag ccgacttttt ccatctcttc
    37921 tttaatacga tgtactacgc gtttttcgtc gcagctttca ctgggaatag caaccatgtc
    37981 gcgcaggaag cgagtcatgt ccgcctggta atcttttgct ttttcaagaa tcagtttgaa
    38041 tggaatattc ttagccatta tttttctcca gattttttac tccccgtttt gcagggagta
    38101 gttccaatag ggtgattaag gtgctacagc gtgtttgcct tcccagacaa cttcgcggta
    38161 gtgcttcacg tcggtgtcgc cttcagtgct gataaccagc actacggcat ctttgtccag
    38221 cgccagtttt tccatcaggc tttgatgttg cgggtgataa tgaaccgctg cgagaacgcc
    38281 caaaccgaca gcgccggatt caccggagat gatgcgcggg tcgttgccgt acggattacc
    38341 cagcacgcgc atacctaatg cggcaacgct gtcctggcag gagatgaatt gggtggcgca
    38401 gttacgtagg atttcccagc ccagcgggtt aggttcgcca caggccaggc ccgccatgat
    38461 ggtggccata tcaccgccaa cgttgacgat gtcgcctttg acgccggagc gataaataca
    38521 gtcagctttg tcaggttcaa caataatgct gtgcagattt tgcgggctgt agacgtcgac
    38581 cagataaccc agcacaccac cggccattgc tccgacaccg gcttgcagca gaacgtgcgt
    38641 cggggttacg cccatttcac gcatttgctc gacggcttca tctgccaggg ttgcgtagcc
    38701 ttgcatgatc caggttggga ttttggtgta accttcccat gccgtgtcct gtaccacttc
    38761 ccagccgtgc tgctgcgcgt gttgcatggt caggcgaacg gtatcgtcat agttcatatc
    38821 cgtgacgatg cactcggcac cgaggttcag aatggcgtca acgcgttcct gagcagaacc
    38881 tttcggcatg taaatcaccg cattctgtcc gagttgctgt gctgcccacg ccacaccgcg
    38941 cccgtggttg ccgtcggtgg tggtcgcgaa agtcattttt tcgccgatgg catttttcag
    39001 gtgctcaaat gacagcgttt cgatatcaag atgatatttt tcacacaata attgagcgat
    39061 ggcgtacgca ccgccaagca ttttgaaggc gttcagaccg aatcgttttg attcgtcttt
    39121 gacgagaatt ttcttcacac caaaaaggtt tgcgagatcg tccagcgcac aaagcggtgt
    39181 tgggcgataa ccagctattt tctggtggaa ctggcgcgcc agtttggcct ggctttgcga
    39241 aaagagcggt gatgtttcgc cgttgaaaaa tttgttatcg gcgatatcaa tcttcaatga
    39301 gaaaacggac atatcctatc cttttggcct ctggaaatag agggccgacc agcagatcgg
    39361 cccgaggggt tatttcacgc gttcttgcgc ttctttcagc agttgctcga gcagtgcacc
    39421 tggtttggcg tatttacggg acaggatcat cgcggcgatg atataaggct tccagctggc
    39481 ttctttgtag gtagcgatac ggtatttttc gaatacgcct tcagtcactt caccttcttt
    39541 gcaggatacg ccgctgatat cagctggcag gcagtgcatg tacagggctt cgccatcacg
    39601 ggtcagttcc atcatctctt cagtacaatg ccagtctttg tgttgcgcgt tctgtgccag
    39661 acactgtttt tccagtgctt ttaagccttc gtggtcgttc gcacgcagca attcagtacg
    39721 ctcttccatc actttgtaag gtgcccatga cttcggataa acgatgtctg cgtctttgaa
    39781 ggcttcttcc atgctggtga cctgacggaa gctaccaccg gaggctttag cattgttttt
    39841 cgccacttca accacatccg ggatcaggtc gtagccttcc ggatgggcca aggtgacatc
    39901 cataccgaag cgagtcatca gaccgatgat gccttgtggt acagagagcg gtttgccata
    39961 gcttggagag taggcccagg tcatggcgat ttttttacct ttcaggtttt ccagtgaacc
    40021 aaagtgttca cgtaaccacg cgaggtcagc cattgactga gtcgggtggt caatatcgca
    40081 ttgcaggttc actaaagccg gacgctgtgg cagtacaccc tgcttgtaac cgtcatcaag
    40141 tgcagcgcca acttcacgca tataggcgtt gcctgcgccc agatacatat cgtcgcgaat
    40201 accaatagcg tcggcgcaga aggagatcat attggcggtt tcacgcacgg tttcgccgtg
    40261 agcgatttgt gatttgcctt cgtcgagatc ttgttgtgca aggccgagca ggttaagcgc
    40321 ggaagcataa gagaagcggg tacgggtgga gttgtcgcgg aatacggaaa tacctaatcc
    40381 actattaaag actttggttg agatgttttc agcacgcagt gcttttaatg ctgcggcaac
    40441 gtccagtact tgtttcagtt catctggcgt ctgttcccac gttaacaaaa aatctttctc
    40501 gtgaaggtga gaggtcagcg aattgatatc cttaatcagc tcattaacag ttttcatcat
    40561 acagtcccgg tagtggaatg acaggcaaag tggtttattt cactgtgact tgccaaagtg
    40621 attggtgttt tcatagctat acctgggact aacaataagt gtgccagaat tatccgcgcg
    40681 gctgcaaaag actaatttga cggataatgt gtggggttgt tcacaaattg atgccagctt
    40741 ctggttgttt gtaaaatacg aaaacgtttg gcttttttta tcgcgttatt tatttgctgc
    40801 taagtatcag aaataagata aaggggcagg ctatctctct tcagtttgat aaactcaccg
    40861 ccgtaattct catattgata tagaaatgtg atatctcttc cataattgca ttagaaaatg
    40921 ataaaaatct tatgttttta tcaaagccaa tttcgcctga gttaatatca tcatgatctg
    40981 tgaaccatca acgtcttcaa tgatcgactt ttaagtaagt ctggaaggta aagctatgga
    41041 gcttgctact acgcagtcag tattgatgca aattcaaccg acaattcagc gttttgccag
    41101 aatgcttgcc agcgttttgc agtttgaggt tgagatcgtt gatgaaaact tgtgtcgcgt
    41161 cgccggaacg ggcgcgtatg ggaagtttct tggtcgccag ttgagcggca actcacgcct
    41221 gctccgccac gtcctggaaa cgaaaactga aaaagttgtg acacagtctc gcttcgatcc
    41281 cctttgcgaa ggttgcgata gtaaagaaaa ttgccgcgaa aaagcatttc tgggtacgcc
    41341 tgtcatttta caggatcgtt gtgttggggt gataagtttg attgccgtta cccacgagca
    41401 acaagagcat atcagtgata atttacgcga attttctgat tatgttcgcc atatatccac
    41461 catttttgtt tcgaaacttc tggaggatca ggggccagga gataacatca gtaaaatatt
    41521 cgcgaccatg atcgataata tggatcaggg cgtattagtt gttgatgatg aaagtcgggt
    41581 tcagtttgtt aatcagactg ccttaaaaac acttggtgtt gtacaaaata atattattgg
    41641 gaaacctatc cgtttcagac cattaacatt tgagagtaat tttactcatg gacatatgca
    41701 gcatattgtt tcgtgggacg ataaaagtga attaatcatt ggtcaattgc ataacattca
    41761 gggccgacaa ttatttttaa tggcatttca ccaatcgcat accagttttt ctgtagcaaa
    41821 tgcacctgat gaaccacata ttgaacaatt ggttggcgag tgccgtgtta tgcggcaatt
    41881 aaaacgactc attagccgta ttgcacccag cccatccagc gttatggtgg ttggtgaaag
    41941 cggcacgggt aaagaagtcg tcgcccgagc aatccataag ttgagcggaa gacggaataa
    42001 accctttatt gctatcaact gtgccgcgat tccggagcag cttctggaaa gcgaactgtt
    42061 cggttatgtt aaaggcgcat ttactagtgc ttctgccaac ggtaaaacag ggttgattca
    42121 ggcggcgaat acgggcacgc tgtttctcga tgaaataggt gatatgccat taatgttgca
    42181 ggctaaatta ctgcgcgcta ttgaggcgcg tgaaattctg ccgattggtg ccagtagccc
    42241 aatacaagtc gacattcgca tcatttctgc aactaatcag aatttggccc agttcattgc
    42301 cgaaggtaaa ttccgcgaag atctcttcta ccgacttaat gttatcccga taactctgcc
    42361 accgctgcgt gaacgtcagg aagatattga actattggtg cattactttt tacatctgca
    42421 tacccgtcgt ctgggatcgg tttatcctgg cattgctccc gatgtcgtcg aaatattgcg
    42481 taagcatcgt tggcccggaa acctgcgcga gttaagcaat ttgatggaat atctggttaa
    42541 cgtggttcct tcaggtgaag ttatcgacag cacgctattg ccgccaaatc tgctgaataa
    42601 tggcacaacg gagcaaagtg atgtaacaga ggtcagtgag gcgcacctgt cactcgatga
    42661 tgcgggcggc acggcgctgg aggagatgga aaagcaaatg atccgcgagg cgctttcacg
    42721 tcataacagc aagaagcaag ttgctgatga actgggcatc ggcattgcta cgctctatcg
    42781 caagattaag aaatatgagt tgttaaacac ataacctcag tgaatccggc ctgaattcag
    42841 gccggatatc cttttacttc gttttctcgc aatccagaac tgtatttaca atcatctgat
    42901 accccgtgca gcgacaaaga tttcccgcca gtccgcgacg aatttccgta atggttaatg
    42961 gcttctcgcg tggtttcgcc agcattgccg tggtagccat aatcaggcca ggcgtacaaa
    43021 acccgcactg cactgcgccg gatttcgcat aagcctgctg aacatgagaa agttttccgc
    43081 ctttcgcttc accttccagc gtgcggatct cctttccttc agcccaggcg gcaaggtata
    43141 agcaactgtc tattgctgtg ccgtcgacca acaccgtaca ggcaccacat tcacccacgc
    43201 agcacccttg tttgacactt agcagtcctt gttcgcggag taattccgag agcggcgtgc
    43261 ctggtgcggc gtgaagctga aaaggcatcc cgttaatggt gcattcgatg gtaattgttt
    43321 cgctgtgatt cattgcaatt ttccccccgc cgcggcgacg gcttcgctaa tcactttttt
    43381 ggtcatcgtc tggatgagat gcagacgaaa ctctttactg gcccgccatg aagaacgcgg
    43441 ggcgacatct tgcaggacag attcgctgat agcttccagc gtttgcaggt ttaatggcgc
    43501 attttgtgca gtctgttcgg catgttggca gcgaatcggc gttggcgcgg caacaccaaa
    43561 tgccaggcgt aattcgctga aattgccgtt atccagtcgg caatgtgcgg cgcagccaat
    43621 cgttgaaata tccattgcgt cgcgcatggc atatttaaaa tgcgcgctgc ccgcgtgttc
    43681 tttcggctgt ggcggaaaat gaaaggcgac gaggatttcg tcatgctcaa gagacacttt
    43741 gcccggcccg gtgtgaaagc cattaatcgg gacgaaacga acaccgcgcg gggagtggat
    43801 ctccagtttc gcgtcataaa ttagcgttgg cgtggcagaa tctgcgctgg tggcaccgtt
    43861 gcaaatattt ccaccgtagg tagcgacgtt acggatctgc ggtccagcaa tggacgtggc
    43921 cgcagcacat aacgccggga gatgacgttg agttatagga gcttctatta gctgggtaaa
    43981 tgtcgttgca gagccgatac gtagcgagcc atcttccgcc agcgtaattc cccgcagctc
    44041 cgccagatta tgaatatcaa caatatggcg ataacggtca ttgtgatggt ggagctgaat
    44101 cagtacgtca gtgccatcgg cgagcagttt ggcctgcggg ttgtcagcca gcaggttgat
    44161 ggcatcggca agggttgctg cgcgatggta agaagcaaaa tcaaacatga tgttatcctc
    44221 aaatcaatcc tgccagatgg aactcttcat ataaccgttt tggcgtcagc ggcagtgtat
    44281 tgattgcaac accggtagcc atcttcaccg cgttacgaat agcagcggca acaggaatta
    44341 ttggtggctc acccagtgac ttatgtccgt atgcggattg cggctcattg atttcgacga
    44401 acgcgctttc cagttgtggc agatccggca tggtcggcat tttgtaatcc agcagattgg
    44461 ggttacggac cacgccgctt ttagcatcga tgatcatctc ttcaaatagc gcccagccaa
    44521 tgcccattcc cattccgccg tgtacctgac cttctgccag cagtggatta agaatatgcc
    44581 ctgaatcatg aacgttgagg atgcggttga tggtgacttt gcacagcgca atatcgaccg
    44641 tcagatcaac aaaggtacag ccaaacgccg atgggttagt ggtggttttg atggagcttt
    44701 cagcagagag ctgcccgccg cgttcagggt ggtagaaagc gtccatcgcc aaatctttta
    44761 acgacattaa cggctcttcc ggtcgttcaa ccagcacgat atggcctttt atcagggtca
    44821 gattcatcgc tgactgatgt agcatgactg cggcgtgagc gatgattttc tcttttaata
    44881 atagtgccgc actgcgcagc gcaggcgcgg caacatagct ctggcgtgag gcaaatgcgc
    44941 cgggagcgaa cggcgtaacg tcggtatctt gagttgaaat aacgcgaacg tcgctgaccg
    45001 gaacccccac ggtttctgcc accatttgcg agaagacggt gtcggcaccc tgaccgattt
    45061 ccgtcgcgcc gctttgcacg ttgatggttc catcctgatt catcagaagg cgcgcgcctg
    45121 ctatttctac gccgacaggc caggtgttag aggtatagct aaaacaggcg acgccaacgc
    45181 cgcggcgcaa attgccttgc tggttctggc attctgcacg gcgtttttcc cattcaaaga
    45241 ttttccggcc tttttcaaga cactccggca accctgcgct gtaaatacgt ttgcccgtga
    45301 gcggattagc atctccttcg cgggcggcgt tgcgtaaacg aatttcaaca ggatcaatac
    45361 ctaacgctgt cgcggcgtca tcaagcatag actcaacggc aaatacgact tgtggcgcgc
    45421 cataaccacg catcgcacca gccgagggga ggttggtata gcaggtcttt gaactgtaag
    45481 cgtaggcaca acgaggataa aggtaagcga ctttattcct ccccgcagaa gcgatggagt
    45541 gcccgtgaga tgcataagcg ccggtgttag acagaacatc cagactataa cctttcaatg
    45601 ttccgtcgcg gttcacgccc atttgcccgt caatggtaaa agcgtggcgg gtacgggttg
    45661 cgaggaaaca ctcttcacgg ctaagggaaa ctttcaccgg aatgccgcca agcttgctgg
    45721 tcaggaatgc tgccattggc tcttccagta catcctgttt attaccaaaa ccgccaccga
    45781 caaacggttt gatgactcgt acgcatgacc agggaatatc cagcgcctga ccaaccacgc
    45841 ggcgaacaat gtgcgggatc tgggtgctgg aaacgatggt aattcgcgag tcatcctcca
    45901 tccacgccag cgatgttacg ctttccatgt gacaatgttg aataacgggg gtctgatagt
    45961 gcccctgtac ctggtagtcg gcggcatcga ttgtttgttg gacattaccc gtcgacatcg
    46021 tgctttgttt cagtaaattg ccaccgttat ggattggtgc agcgtcttct gccagcgccg
    46081 cttctggcgt ggtgataacg ggtaattctt gccactcaat gctgaccaat tgcgccgctt
    46141 tttctgccgt gagttcatcg cgggccacga cgatggcaac ggcgtcacca tgatgacgaa
    46201 catggcgagt tagcagtgcg cgatcggcgg tatcgcgctt gttttcgtca agtgtccagg
    46261 catgcccagc tgtagcgaat ggaatatcag gcacatcttc ccaggtaaaa atcgccagta
    46321 cgcccggcaa acttctggct tgttcatcat taatacttac ggcataacca tgtgcgatag
    46381 ggctacgtac atatttcgca taacacatgc ccgccataac ataatcgtca gtatatcgtg
    46441 cccgcccggt gaccttagca atggcatcga cgcgcatgca tgattcaccc gtagcggttg
    46501 cttcccgcgc ttccatagaa atcccctcga tttgaacatt tggttcttat ggcaaagatt
    46561 tagtctaagc aggcaagatt tacgccagaa acgacgataa gcgttagggg aggtaggaag
    46621 tgtgatgacg ttaaaaaaac acagatatga tacgaaacag gactctgaat aggggcagtg
    46681 atacgtatca atatgagtgg cgataaaatc aatgtgataa attgggatta ttacgcatca
    46741 ggatgggaaa tagggtaaaa agtcaggaag ttcgatacct ctacagcgaa tattttgctg
    46801 atgatacttc cctttgccaa atgaaattaa cctctttctc tattaccacg tttttccaga
    46861 agcaagagat tgggtatcaa gaggctggct gctatgataa ggcgtcttgt tttttaagcg
    46921 aggaaagatt ttgagtgcgg gacgcctgaa taaaaaatct ctgggtatcg tgatgttgtt
    46981 atcggttgga ctgcttttgg cgggctgttc gggtagcaaa tcatccgata caggaacgta
    47041 ttccggctcc gtttacaccg tgaaacgggg ggatacgcta tatcgtattt cgcgcaccac
    47101 gggaaccagc gtaaaagaac tggcgcgact gaacggcatt tccccccctt acaccattga
    47161 agttggtcag aaactaaaac tgggtggggc gaaaagtagc agtagtacac gtaaatcaac
    47221 cgccaaatca acgaccaaaa ccgcatcggt tacaccgtca tcagcggtac cgaaatcatc
    47281 ctggccgcca gtagggcaac gttgttggtt atggccaacg acagggaaag ttatcatgcc
    47341 gtattcgaca gcagatggcg gcaataaagg gattgatatc tcagctccac ggggtacacc
    47401 tatttacgcc gcgggtgcag gaaaggtggt gtatgtgggc aaccagctgc gtggctacgg
    47461 taatctcatc atgattaaac acagtgaaga ttacattacg gcttacgccc ataatgacac
    47521 gatgctggta aataatgggc aaagcgtgaa ggctgggcaa aaaatcgcca ctatggggag
    47581 cacggatgcg gcatctgttc gcctgcattt ccagattcgt taccgtgcaa cggcaattga
    47641 tccgctacgt tacttgccgc cgcagggcag caagccaaaa tgctgatggc gaattaatca
    47701 gcagtcagca gcgcggcact tgctaaggag agcgtaaggt ttataatgcc ttacgcatct
    47761 cgaagcgggc gtagttcaat ggtagaacga gagcttccca agctctatac gagggttcga
    47821 ttcccttcgc cgcttttgct gtatctgatt ttctatatta tcttaattca atcgcttcag
    47881 acttttcgca atcgccgtaa tatttcccca ttttgttgta aataaaaagc gtttacgcaa
    47941 tgcaatcctt ttgtaacaac ccaggtaaaa caccttattt tcaagttgtt aaataagtgg
    48001 ttatttacgc ttttaacaaa cgattgatgg aatattttat gaaagggaaa tctgcattaa
    48061 ctcttctgct cgctggtata ttttcgtgcg gcacatgcca ggcgacaggg gcagaagtga
    48121 cgagtgaatc tgtatttaac attcttaatt ccacgggggc agcaacagat aaaagttatt
    48181 tatcgttaaa tccagataag taccctaatt atcgtcttct gattcactca gccaaattaa
    48241 aaaatgaaat aaagagccac tataccaaag acgaaattca agggttgtta acattaacag
    48301 aaaacacacg aaagttaacg ttgacggaga aaccctgggg aacatttatt cttgcttcta
    48361 ctttcgaaga tgacaaaaca gcggcagaaa cccactatga tgctgtctgg ctgagggata
    48421 gtttatgggg ctatatggca ttagtatcag atcagggaaa tagtgtagcc gcaaaaaagg
    48481 ttctgctcac cctgtgggat tatatgtcta cgctcgacca gattaagcgg atgcaggacg
    48541 tcattagcaa ccccaaacga ctggatggag ttcccgggca aatgaatgct gtgcatatcc
    48601 gatttgacag caactccccc gtgatggcag atgtgcagga agaaggcaag ccgcagctgt
    48661 ggaaccataa acaaaatgat gcgttaggtc tttatcttga tctccttata caagcaatcg
    48721 acactggtac aattaacgct gaagattggc aaaaaggtga ccgattaaaa tcggttgcgc
    48781 tgctgattgc ttatctggat aaagccaatt tctatgttat ggaagattcc ggagcatggg
    48841 aagaagatgc ccgattaaac acatcttccg ttgcactggt gacctctgga ctggaacgtt
    48901 tgtcgaactt actctcgaag aaagactctg tttttgtctc tgatttacta cgcgaagcta
    48961 aagccaatga actggatgaa cctctttcaa caacgaggtt gaaccacctc atagataaag
    49021 gttatgaaag aataacgtta cagcttgatt tagggggaga atccccaggt tatctggaaa
    49081 aagataaaca ttacagagaa gccgatgcgg ctttacttaa tgttatttat cctgcaaatc
    49141 ttgcaaagat aaacaccaga agaaaagagc aggttctaaa aatagttaaa aaactagcag
    49201 gtccgtatgg tataaaaaga tacgagaagg ataattatca gtcggcaaat ttttggttca
    49261 atgatattaa aaccgatacc gatcaaaatt cacatgcgaa aag
//
LOCUS       DAZPDU010000007        48837 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZPDU010000007 DAZPDU010000000
VERSION     DAZPDU010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48837
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.007"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            147..1490
                     /gene="argG"
                     /locus_tag="ACHURS_000360"
     CDS             147..1490
                     /gene="argG"
                     /locus_tag="ACHURS_000360"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFR1236184.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENLENKGQ"
     gene            complement(1498..3123)
                     /locus_tag="ACHURS_000361"
     CDS             complement(1498..3123)
                     /locus_tag="ACHURS_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFR1236185.1"
                     /translation="MTVFNKFARSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQSISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPKTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(3582..3668)
                     /locus_tag="ACHURS_000362"
     tRNA            complement(3582..3668)
                     /locus_tag="ACHURS_000362"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3632..3634),aa:Leu,seq:gag)
     gene            complement(3683..4015)
                     /gene="secG"
                     /locus_tag="ACHURS_000363"
     CDS             complement(3683..4015)
                     /gene="secG"
                     /locus_tag="ACHURS_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFR1236186.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(4243..5580)
                     /gene="glmM"
                     /locus_tag="ACHURS_000364"
     CDS             complement(4243..5580)
                     /gene="glmM"
                     /locus_tag="ACHURS_000364"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFR1236187.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(5573..6421)
                     /gene="folP"
                     /locus_tag="ACHURS_000365"
     CDS             complement(5573..6421)
                     /gene="folP"
                     /locus_tag="ACHURS_000365"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFR1236188.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(6511..8445)
                     /gene="ftsH"
                     /locus_tag="ACHURS_000366"
     CDS             complement(6511..8445)
                     /gene="ftsH"
                     /locus_tag="ACHURS_000366"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFR1236189.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(8545..9174)
                     /gene="rlmE"
                     /locus_tag="ACHURS_000367"
     CDS             complement(8545..9174)
                     /gene="rlmE"
                     /locus_tag="ACHURS_000367"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFR1236190.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            9300..9593
                     /gene="yhbY"
                     /locus_tag="ACHURS_000368"
     CDS             9300..9593
                     /gene="yhbY"
                     /locus_tag="ACHURS_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFR1236191.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(9749..10225)
                     /gene="greA"
                     /locus_tag="ACHURS_000369"
     CDS             complement(9749..10225)
                     /gene="greA"
                     /locus_tag="ACHURS_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFR1236192.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            10473..11906
                     /gene="dacB"
                     /locus_tag="ACHURS_000370"
     CDS             10473..11906
                     /gene="dacB"
                     /locus_tag="ACHURS_000370"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFR1236193.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAQKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            11961..12068
                     /locus_tag="ACHURS_000371"
                     /pseudo
     CDS             11961..12068
                     /locus_tag="ACHURS_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12092..13264)
                     /gene="cgtA"
                     /locus_tag="ACHURS_000372"
     CDS             complement(12092..13264)
                     /gene="cgtA"
                     /locus_tag="ACHURS_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFR1236194.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFILE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(13280..14245)
                     /locus_tag="ACHURS_000373"
     CDS             complement(13280..14245)
                     /locus_tag="ACHURS_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR1236195.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(14372..14629)
                     /gene="rpmA"
                     /locus_tag="ACHURS_000374"
     CDS             complement(14372..14629)
                     /gene="rpmA"
                     /locus_tag="ACHURS_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFR1236196.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(14650..14961)
                     /gene="rplU"
                     /locus_tag="ACHURS_000375"
     CDS             complement(14650..14961)
                     /gene="rplU"
                     /locus_tag="ACHURS_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFR1236197.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            15220..16191
                     /gene="ispB"
                     /locus_tag="ACHURS_000376"
     CDS             15220..16191
                     /gene="ispB"
                     /locus_tag="ACHURS_000376"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFR1236198.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            16419..16697
                     /gene="sfsB"
                     /locus_tag="ACHURS_000377"
     CDS             16419..16697
                     /gene="sfsB"
                     /locus_tag="ACHURS_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFR1236199.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(16745..18004)
                     /gene="murA"
                     /locus_tag="ACHURS_000378"
     CDS             complement(16745..18004)
                     /gene="murA"
                     /locus_tag="ACHURS_000378"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFR1236200.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(18059..18313)
                     /gene="ibaG"
                     /locus_tag="ACHURS_000379"
     CDS             complement(18059..18313)
                     /gene="ibaG"
                     /locus_tag="ACHURS_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFR1236201.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(18473..18766)
                     /gene="mlaB"
                     /locus_tag="ACHURS_000380"
     CDS             complement(18473..18766)
                     /gene="mlaB"
                     /locus_tag="ACHURS_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFR1236202.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(18766..19401)
                     /gene="mlaC"
                     /locus_tag="ACHURS_000381"
     CDS             complement(18766..19401)
                     /gene="mlaC"
                     /locus_tag="ACHURS_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFR1236203.1"
                     /translation="MFKRLMMVALLVIAPLSAATEADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(19420..19971)
                     /gene="mlaD"
                     /locus_tag="ACHURS_000382"
     CDS             complement(19420..19971)
                     /gene="mlaD"
                     /locus_tag="ACHURS_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFR1236204.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(19976..20758)
                     /gene="mlaE"
                     /locus_tag="ACHURS_000383"
     CDS             complement(19976..20758)
                     /gene="mlaE"
                     /locus_tag="ACHURS_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFR1236205.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(20766..21575)
                     /gene="mlaF"
                     /locus_tag="ACHURS_000384"
     CDS             complement(20766..21575)
                     /gene="mlaF"
                     /locus_tag="ACHURS_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFR1236206.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            21785..22762
                     /locus_tag="ACHURS_000385"
     CDS             21785..22762
                     /locus_tag="ACHURS_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFR1236207.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIEPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            22776..23762
                     /gene="kdsD"
                     /locus_tag="ACHURS_000386"
     CDS             22776..23762
                     /gene="kdsD"
                     /locus_tag="ACHURS_000386"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFR1236208.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            23783..24349
                     /gene="kdsC"
                     /locus_tag="ACHURS_000387"
     CDS             23783..24349
                     /gene="kdsC"
                     /locus_tag="ACHURS_000387"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFR1236209.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            24346..24921
                     /gene="lptC"
                     /locus_tag="ACHURS_000388"
     CDS             24346..24921
                     /gene="lptC"
                     /locus_tag="ACHURS_000388"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFR1236210.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGIDMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            24890..25447
                     /gene="lptA"
                     /locus_tag="ACHURS_000389"
     CDS             24890..25447
                     /gene="lptA"
                     /locus_tag="ACHURS_000389"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFR1236211.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            25454..26179
                     /gene="lptB"
                     /locus_tag="ACHURS_000390"
     CDS             25454..26179
                     /gene="lptB"
                     /locus_tag="ACHURS_000390"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFR1236212.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            26227..27660
                     /gene="rpoN"
                     /locus_tag="ACHURS_000391"
     CDS             26227..27660
                     /gene="rpoN"
                     /locus_tag="ACHURS_000391"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFR1236213.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDIGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHINTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            27683..27970
                     /gene="hpf"
                     /locus_tag="ACHURS_000392"
     CDS             27683..27970
                     /gene="hpf"
                     /locus_tag="ACHURS_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFR1236214.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            28088..28579
                     /gene="ptsN"
                     /locus_tag="ACHURS_000393"
     CDS             28088..28579
                     /gene="ptsN"
                     /locus_tag="ACHURS_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFR1236215.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVSADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            28625..29479
                     /gene="rapZ"
                     /locus_tag="ACHURS_000394"
     CDS             28625..29479
                     /gene="rapZ"
                     /locus_tag="ACHURS_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFR1236216.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            29476..29748
                     /gene="npr"
                     /locus_tag="ACHURS_000395"
     CDS             29476..29748
                     /gene="npr"
                     /locus_tag="ACHURS_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFR1236217.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            29962..30594
                     /gene="yrbL"
                     /locus_tag="ACHURS_000396"
     CDS             29962..30594
                     /gene="yrbL"
                     /locus_tag="ACHURS_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFR1236218.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKLSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(30591..31319)
                     /gene="mtgA"
                     /locus_tag="ACHURS_000397"
     CDS             complement(30591..31319)
                     /gene="mtgA"
                     /locus_tag="ACHURS_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFR1236219.1"
                     /translation="MSKSRLTVFSFVRRFLLLLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(31316..31969)
                     /gene="elbB"
                     /locus_tag="ACHURS_000398"
     CDS             complement(31316..31969)
                     /gene="elbB"
                     /locus_tag="ACHURS_000398"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFR1236220.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(32199..34535)
                     /gene="arcB"
                     /locus_tag="ACHURS_000399"
     CDS             complement(32199..34535)
                     /gene="arcB"
                     /locus_tag="ACHURS_000399"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFR1236221.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(34631..35560)
                     /locus_tag="ACHURS_000400"
     CDS             complement(34631..35560)
                     /locus_tag="ACHURS_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFR1236222.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLINRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTVHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGLAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            36235..40695
                     /gene="gltB"
                     /locus_tag="ACHURS_000401"
     CDS             36235..40695
                     /gene="gltB"
                     /locus_tag="ACHURS_000401"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFR1236223.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGHILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFCQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            40708..42126
                     /gene="gltD"
                     /locus_tag="ACHURS_000402"
     CDS             40708..42126
                     /gene="gltD"
                     /locus_tag="ACHURS_000402"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFR1236224.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            42310..42714
                     /locus_tag="ACHURS_000403"
                     /pseudo
     CDS             42310..42714
                     /locus_tag="ACHURS_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417687.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
     gene            42806..43111
                     /locus_tag="ACHURS_000404"
     CDS             42806..43111
                     /locus_tag="ACHURS_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1236225.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(43187..43651)
                     /gene="nanQ"
                     /locus_tag="ACHURS_000405"
     CDS             complement(43187..43651)
                     /gene="nanQ"
                     /locus_tag="ACHURS_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFR1236226.1"
                     /translation="MMMGEVRSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIENEQAIILKPGMFAVFMLGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(43648..44523)
                     /gene="nanK"
                     /locus_tag="ACHURS_000406"
     CDS             complement(43648..44523)
                     /gene="nanK"
                     /locus_tag="ACHURS_000406"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFR1236227.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELMGADARTIFTRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAVFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(44520..45209)
                     /locus_tag="ACHURS_000407"
     CDS             complement(44520..45209)
                     /locus_tag="ACHURS_000407"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFR1236228.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPIPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(45257..46747)
                     /gene="nanT"
                     /locus_tag="ACHURS_000408"
     CDS             complement(45257..46747)
                     /gene="nanT"
                     /locus_tag="ACHURS_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFR1236229.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(46857..47750)
                     /gene="nanA"
                     /locus_tag="ACHURS_000409"
     CDS             complement(46857..47750)
                     /gene="nanA"
                     /locus_tag="ACHURS_000409"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFR1236230.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(47872..48663)
                     /gene="nanR"
                     /locus_tag="ACHURS_000410"
     CDS             complement(47872..48663)
                     /gene="nanR"
                     /locus_tag="ACHURS_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFR1236231.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGEFSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT        12167 a        13134 c        12067 g        11469 t
ORIGIN      
        1 aagcgcatta ttttacaaaa aacacactaa actcttcctg tctccgataa aagatgatta
       61 aatgaaaact catttatttt gcataaaaat tcagtgagag cggaaatcca ggctcatcat
      121 cagttaatta agcagggtgt tattttatga cgacgattct caagcatctc ccggtaggtc
      181 aacgtattgg tatcgctttt tccggcggtc tggacaccag tgccgcactg ctgtggatgc
      241 gacaaaaggg agcggttcct tatgcatata ctgcaaacct gggccagcca gacgaagagg
      301 attatgatgc gatccctcgt cgtgccatgg aatacggcgc ggagaacgca cgtctgatcg
      361 actgccgcaa acaactggtg gccgaaggta ttgccgctat tcagtgtggc gcatttcata
      421 acaccaccgg cggcctgacc tatttcaaca cgacgccgct gggccgcgcc gtgactggta
      481 ccatgctggt tgctgcgatg aaagaagatg gcgtgaatat ctggggtgac ggtagcacct
      541 ataaaggaaa cgatatcgaa cgtttctatc gttatggtct gctgaccaat gctgaactgc
      601 agatttacaa accgtggctt gatactgact ttattgatga actgggcggc cgtcatgaga
      661 tgtctgaatt tatgattgcc tgcggtttcg actacaaaat gtctgtcgaa aaagcctact
      721 ccacagactc caacatgctt ggtgcaacgc acgaagcgaa ggatctggaa tacctcaact
      781 ccagcgtcaa aatcgtcaac ccaattatgg gcgtgaaatt ctgggatgag agcgtgaaga
      841 tcccggcaga agaagtcaca gtacgctttg agcaaggtca tccggtagcg ctgaacggta
      901 aaacctttag cgacgacgta gaaatgatgc tggaagctaa ccgcatcggc ggtcgtcacg
      961 gcctgggcat gagcgaccag attgaaaacc gtatcatcga agcgaaaagc cgtggtattt
     1021 acgaagctcc ggggatggca ctgctacata tcgcttatga acgtctgttg accggtattc
     1081 acaacgaaga caccattgag cagtatcatg cacatggtcg tcagttgggc cgtctgctgt
     1141 accaggggcg ttggtttgat tcccaggcgc tgatgctgcg tgactctctg caacgctggg
     1201 ttgccagcca gatcactggt gaagttaccc tggagctgcg ccgtgggaac gattattcaa
     1261 tcctgaatac cgtctcagag aacctgacct acaagccaga gcgtctgacg atggaaaaag
     1321 gcgactcggt gttctcgcca gatgatcgta ttggtcaatt gaccatgcgt aacctggata
     1381 tcactgatac ccgagagaaa ctttttggtt atgccaaaac tggcctgctc tcctcctctg
     1441 ccacttcagg cgtgccgcag gtggagaatc tggaaaacaa aggccagtaa ttcgacatca
     1501 accctgccct tcaggggtgg ggttaccttg cttatcctct gtaaactcct ttctgagcat
     1561 cacataatcg aaaacatcat ggtttgcatc cacaacccgt gagtctcctt tatagtgtgc
     1621 aatcacttcc tccagcgttt tcggttgttc cggctttgtt acccccatcc acgcattaat
     1681 aaggtaatta ttgtaagctg tcgaaaagat gttgttttcc gttgtacgat ccacgccatc
     1741 cccaagaacg gggctatacc agataaacat cgggacatga tatgcctgct ggctagcctc
     1801 cctgcctcca tgaaaataga cgttcttctt cgtagggtca cgttccaggc cgtggtcggc
     1861 aaaatacatg accgaggcgc ggcgatcttt taataattca aaaacctgac ctagcaaact
     1921 atctgtgtaa tgaatggagt tgtcatagca ggcatcctga tcgtcctgcg gttgaaacac
     1981 ggcgctggat tgcggatagg cgctacaagc cggttcatgg cttccattta aatgaagaac
     2041 aatcagtttc ttttgctgcg tattttgctg taatgcttgc gataaatgcg gcaacaacaa
     2101 ttcatcaaat cctctgacat aaactgtttc catggcgcgc atggcgatac tggtaactgc
     2161 tgtaccattc tgccgaaaag cggattgcga gcttagccag aaagtctgaa atcctgcttg
     2221 attagccata ttaataatgt tgtcggggta attatgaatg tcatgactca aaacagaatc
     2281 agcagttaaa gaaaggggaa ccgacagcgc agtgtaaggt gcgccgctta ttgattgatt
     2341 aaacagtttg atctgttttc tttgtgcttc aacttgcggt gtcgtagagc gtgtatatcc
     2401 atacaaagac atattgtcga cacgtacaga ctcccccaca atcaacacgt aggtatcaat
     2461 acctgtatcc ctgactgata attgaaaata cggcaccgtg tttgcaattg agagtaatct
     2521 ttgatgctct ttcgctgcta aagcaaaata attgagattg aaaaacggcg tataggtagc
     2581 aaatcgcgac gctagtatat atggactgaa tgctttttta ttttttgcat ctttataagc
     2641 aaattgacaa gcagaaaata aactgcccga aatgacaatc agcaataata ttcctgtcac
     2701 tttttttgtc ggcaaggaaa catcatattt tattattact gccaaaaaaa gaagggataa
     2761 aaaagcaaaa gcacatagat aagggatata catccccagc attttgacaa cttcatccgg
     2821 atcactctgc agcacactaa tcgcaaatcc atcattgaaa gttgtaccaa aggtacacca
     2881 tgaatagagg ctaatactca tgtctgccgc agtcagtaac acaaatggtg cggcaacaag
     2941 caggcgaagc ggaagggatg atatacgttt tacaaccagg atggtcagga cgaagaacag
     3001 caatcgctga accatatgcg cgccggaaga ggcgactaag ttcgtaatac caaaaacaat
     3061 aacacaaaga tacaacaacc aatgagattt aaaacttcta gcaaatttgt tgaatactgt
     3121 catccagaaa tccaagttca caaccaatat aaacaattat caacaatgtt caattttata
     3181 tcagacatgt ttttcaactc tgatccatat cagaccgaaa cactgttagt tttttctttt
     3241 taaatgatgc ggccattgcg ttctcagtaa ttaaataaca ttatcaatgc gtctctaaac
     3301 attacgcaga acgtagccat cagtagttat actgacagga gcaggatggt ttatcgagaa
     3361 gtaaacatat ccgtattcgt accggaactg gagttgacac aataaagtgc caattatgtc
     3421 agcagaaggg aaaagtttat aactaaggcg taccggcacc atcgtttcaa ggtaccagct
     3481 acgagtaaag caactggacg agatacagat acctgacaac cattcctcag accaggacca
     3541 aaacgaaaaa agacgctttt cagcgtctct tttctggaat ttggtaccga ggacgggact
     3601 tgaacccgta agccctattg ggcactacca cctcaaggta gcgtgtctac caattccacc
     3661 acctcggcac ggatactact ttttagttcg ggatatcgct ggtcggctta gccggagcag
     3721 ctggctgagt ttgttcggtt ttcgccggtg cactcagatt ttcccattcg ctacctttat
     3781 tggttttgtt gctattgatg ttacccagca ccagactgat gatgaagaat aacgttgcca
     3841 gcagcgccgt catgcgggtc atgaagttac cagaaccact tgaaccaaac agcgtagcgg
     3901 aagcgcctgc tccgaaggag gctcccatat cagcgccttt accttgctgc agcatgatca
     3961 gaccaacaag gccaattgcc acaataagga aaactaccaa aagagcttcg tacataatca
     4021 acctgttcct tgcggagttg ccgcgtacca atgcttcaac caataaagcg ggagttttta
     4081 atctttccca ctgaagtggg tgtgaatact aaccaaagcg aatgaccttc gcaagggcaa
     4141 ttttatcgca ttgtatcaac tgcggaaaaa aacagcaaaa ccgatgtgtt cgctgagaaa
     4201 aaaggcgaca atcgccgcct ttttagccag ttatttaccg ttttaaacgg cttttactgc
     4261 atcggcgata cggtgtgcaa attcagttac ctgcgcttcg tcttcacctt ctaccatcac
     4321 gcgaattaac ggttcggtgc cggatttacg caacagcaca cgtccacggc tacccagcgc
     4381 cgcttcaact tcggcggtca cagctttaac tgactcatgc tcaagcggat cgccgctacc
     4441 tgcggtgtaa cgcacgttaa ccagaatctg cgggaacatt ttcatgccgc tgcaaaggtc
     4501 gtgcaggctc atatggttac gtgccatcgc cgccagcacc tgcaagccag caacgatgcc
     4561 gtcaccggta gtggttttat ccagcaggat cacatgaccg gaattctctg caccgatacg
     4621 ccagcctttc tcctgcattt tttccagtac gtagcggtca cccactttcg cgcgcgcaaa
     4681 tggaattcct aactgtttca acgccagctc aagccccatg ttactcatca atgtacccac
     4741 agcaccacca cgcagctggc cctgacgaag accttcacgc gcgatgatat acatgatctg
     4801 atcaccatcg actttattgc cttcatggtc aaccataatc acgcgatcgc cgtcgccgtc
     4861 gaaggcaata ccgagatccg ctttttcagc cagcacacga gcctggagcg cgcgaacgtc
     4921 ggtagccccc acttcggcat tgatgtttac accgttcggc tcacaaccga tagcgataac
     4981 gttcgccccc agttcacgca gcacgttcgg cgcgatgtga taagtcgcac cgtttgcgca
     5041 atccaccaca atcttcagtt cgctgaggct aagttcgttc gggaacgtgg ctttgcaaaa
     5101 ctcgatatag cgacccgcag catcaacgat acggctggct ttacccagtt ctgccgaatc
     5161 aacacagctg atctcttttt ccatttccgc ttcgatggcc tcttctaccg catccggtag
     5221 tttggtgccg tcgatagaga aaaatttaat gccattatcg tagaacgggt tatgcgatgc
     5281 agatatcaca attccggcct ctgcgcggaa ggtacgcgtc agataagcca cggccggtgt
     5341 tggcatcggg ccagtgaaga gtgcggaaag gcccgctgcc gccagacccg cttccagtgc
     5401 tgactccagc atatagccag aaatacgcgt gtctttacca ataatgatct tacgggagcc
     5461 gtggcgcgcc agcactttac ccgcggccca acccagctta agcacaaaat caggtgtgat
     5521 cggcgcatcc cctacacgac cacgaatccc atcggtaccg aaatatttac gattactcat
     5581 agcgtttgtt ttcctttgca gacagagtgg cttccaccac ccgcatcgct tctacggttt
     5641 ctttgacgtc atgaacacga ataatgtgcg cgccttgcat tgcggcaatg accgcacagg
     5701 ccagactacc gctcaggcgc tcggacggcc ccacgttcag cagctgccca atcatcgatt
     5761 ttcgtgacat acctaccaac agcggcaggt tgaaatggtg aaattcagcc aggcgcgcca
     5821 gtaatgaata gttatgggag agatttttac cgaaaccgaa tccggggtcg agcaacaatt
     5881 tctcttttgc gatacccgcc tgctcgcaac gtgctatttg ctcaataaag tagcgattca
     5941 cttctgcaaa gacatcgtca tacttcggag cttcctgcat ggtttttgga tttccctgca
     6001 tatgcatcag acaaaccggt aaaccggttt ctgcagccgc ctccagagcg ccaggttcgg
     6061 aaagggagcg gatatcgtta ataatgtgag cgccaacttt cgctgactca cggatgactt
     6121 ctggtttgga tgtatctacc gagatccaga cttcgaagcg ttgagcaatt gcctcaacca
     6181 caggaataac acgttgcaac tcttcttcaa cgctaacttc cgccgcccct gggcgcgtgg
     6241 actcgccacc aacgtcaatg atcgtcgcgc cagcgttgat catcagattc gcatgtttca
     6301 ccgcatctat cagcgagtta tgcgtgccac catccgaaaa ggaatcaggc gtgacgttga
     6361 ggatccccat tacgtgagga tggctaaggt ccagtgaagt gccctgggca aagagtttca
     6421 tggtgttatc cctggtatga attgataaga aaaaaccccg gagcacgccc cggggttttc
     6481 ggtacaaata cagtcatctg atgcgggaac ttacttgtcg cctaactgct ctgacatggt
     6541 gttacccggg ttcggcgtac gcggttcatc aaccggacga ggagcctttg gactaccatt
     6601 gtcgccagaa ttgttagaag cgcctggttc ttcccagccc gctggcggac gtacatcgcg
     6661 acgtgccatc aggtcatcaa tctgcggtgc gtcgatagtc tcatatttca tgagagcatc
     6721 tttcatcgca tgcagaatat ccatattgtc ggtcagaagc tgacgcgcac gattatagtt
     6781 acgctcaatc agtgctttca cttcctggtc gatgatacgt gcagtttcat cggacatatg
     6841 tttcgctttc gctacgctac ggccgaggaa cacttcacct tcttcttccg cgtacagcag
     6901 tggacccaat ttctcagaga agccccactg agtcaccatg ttacgcgcca ggttggtcgc
     6961 aactttaata tcgttggacg caccggtaga aacatgttcc ggcccgtaga tgatctcttc
     7021 tgccagacga ccaccgtaca gcgtagaaat ctggctttcc agtttctgac ggctggcgct
     7081 gattgcgtcg ccctcaggca agaagaaagt cacacccagc gcacgaccgc gtgggataat
     7141 cgtcactttg tgcaccggat cgtgttccgg caccaggcga ccgataatcg catgacccgc
     7201 ttcgtggtaa gccgtcgatt ctttctgcgc ttccgtcatc accatggagc gacgttccgc
     7261 acccatcatg attttatctt tcgctttctc gaactcaacc atcgacacaa cgcgtttgtt
     7321 gccacgagca gcgaacagtg ccgcttcgtt taccaggttc gccaggtcag caccggagaa
     7381 accaggagta ccacgggcaa tgattgccgc gtcgatatcg ggtgccaatg gtacgcgacg
     7441 catgtgaact ttcaggatct gctcacgacc gcgaacatct ggcaagccaa ccacaacctg
     7501 acggtcgaaa cggccaggac gcagcagggc cgggtcaaga acgtccggac ggttagtcgc
     7561 ggcgataacg atgatacctt cgttaccttc gaagccatcc atctcaacca gcatctggtt
     7621 cagagtctgt tcacgttcat cgtgaccacc gcccagacca gcgccacgct ggcggcctac
     7681 ggcgtcgatt tcatcgataa agatgatgca cggtgccgct ttcttcgcct gttcgaacat
     7741 gtcacgaaca cgggatgcac ccacaccgac gaacatttct acgaagtcag aaccggagat
     7801 agtaaagaac ggaactttcg cttcgcctgc aatcgctttc gccagcagcg ttttaccggt
     7861 acccggagga ccgaccatca ggacgccttt cgggatctta ccgccgagtt tctggaagcg
     7921 gctcggctcg cgcagatatt caaccagttc agcaacttct tcttttgctt cgtcgcagcc
     7981 cgcaacgtca gcaaaggtcg ttttgatctg atcttccgtc agcatgcgcg ctttgctctt
     8041 accaaacgac atggcacctt tgccaccgcc gccctgcatt tgacgcatga agaagatcca
     8101 cacaccaatc agcaacagca tcgggaacca ggagatgaag atagaagcca gcaggcttgg
     8161 ttcttcaggc ggttcaccga caaccttgac gttcttggtc aacaggttat ccagtaattt
     8221 cggatcctga accggaatgt aagtggtata acggttacta tctttcttgg taacgttgat
     8281 ttcacgtccg ttgatacgcg cttcacgaac ctggtcgtta ttgacctctt gtaggaaggt
     8341 agagtaatcc accttacggc cattagactc gctgggccca aagctctgga atactgacat
     8401 cagcacaacg gcaatgacca gccagagtat taggtttttc gccatgtcac tcaagggatt
     8461 aacctcttat tacaactgtg ttaaaaacag cgtcaggata ctctatatcc agcatctttc
     8521 aaactttcgt ctgaaatctc ccggttaggg tttacgcccg gtcgctacaa tatacacttc
     8581 ccgcgaacgt gcacgagaag agtccggctt acgaactttg accttcgtaa acagggagcg
     8641 aatttccctt agatactcat cgaaaccttc gccctggaac accttcacta caaaactgcc
     8701 acctggcgct aatacatcac gacacatttc tagcgccagt tccaccagat acatggcacg
     8761 ggggatatcc accgccggtg ttccgctcat gtttggtgcc atatcggaca tgacaacctg
     8821 gactttgctg tcgccaacgc gctccagcag tgctttcatc accagttcat cacgaaaatc
     8881 gccctgaaga aagtccacac caacgatagg atccataggt aaaagatcgc aagcgatgat
     8941 gcggcctttg ccgccaattt gggtgaccac atattgtgac caaccacccg gagcagcacc
     9001 aaggtcgaca accgtcattc ccggtttaaa gagtttgtca ctttgctgta tttcatcaag
     9061 tttaaaccag gcacgggaac gtaacccctt tttctgtgcc tgttgaacat atttatcgct
     9121 aaagtgttcc tgaagccagc ggctggagct ggcagaacgc ttcttacctg tcatttaact
     9181 ttcccatggg gataactcat cgtaaccaat tgcgtaaatt tttacgcgcc tatttggtga
     9241 tatatgggag atggcggtag aatgacccat tttcaatccc aacgtaagca aaaaatacga
     9301 tgaatctgag tactaaacaa aaacagcacc tgaaaggtct ggcacatccg ctcaagccag
     9361 ttgttctgct tggcagtaat ggtttgaccg aaggggtgct ggccgagatt gaacaagcgt
     9421 tagagcacca tgaactcatc aaggtgaaaa tcgccaccga agatcgcgaa acaaaaacct
     9481 tgatcgtgga agctatcgtg cgcgaaaccg gcgcctgtaa tgtacaggtc atcggtaaaa
     9541 cgctggtgct ttatcgccca actaaagaac gtaaaatctc gctgccacgc taagattatc
     9601 ctaaagttac acacatttgc tgtgtaaaac gaggggtttt ccgcaggcag gagagcaaaa
     9661 tgccacgctc tgttcgttga taaaaggccg catagcggcc tttttccttt ctttacaata
     9721 catcaacatc ttgagtattg ggtaattctt acaggtattc caccttaatt acttcaaatt
     9781 ctacttcgcc gcccggcgtt ttgatgacca caacatcatc ttcttctttg ccgatcaggc
     9841 cacgagcaat aggcgagttt acagaaatca ggttttgttt aaagtcagct tcgtcatcgc
     9901 caacgatgcg ataagtctgt tcttcgtcag aatccagatt cagcaccgtt acggtagcac
     9961 caaaaataac gcgcccattg ttaggcattt tggtgacatc aatcacctgc gcgtttgaca
    10021 gcttggcttc gatgtctttg atacggcctt cgcagaagcc ctgctgttca cgagctgcgt
    10081 ggtattcggc gttttctttc aggtcgccat gctcacgcgc ttccgcgata gcagcaatga
    10141 tttcaggacg gcgcacagat ttcagaaaat ccagctcttc gcgtaatttt tcagcgccgc
    10201 gtaaggtcat cggaatagct tgcatttgtt atacctcttg aatattcctg atggggcaag
    10261 tcttcaccca ccccggctgt tcggcctgcc cggcataact cctcaccggg accagaagca
    10321 aaaaaatacc gacccaggta caagtcccag gtcagctaca attcacattt tgatagtcat
    10381 tttaccctga agttcccgaa gggtcatcgt ttactttata gggcgttgcg ccgtagtatg
    10441 acggctcgat tccaggttgt tagcgcgaga ttatgcgatt ttccagattt atcatcggat
    10501 tgaccagctg tatagcgttc agtgttcagg ccgcaaatgt tgatgagtac attactcaac
    10561 tccccgctgg tgccaacctt gccctgatgg tgcaaaaagt cggcgcgtcg gcccccgcta
    10621 ttgattacca cagtcagcag atggcgctgc ctgccagtac ccagaaagtg attactgcgc
    10681 tggcggcgtt gattcaactc ggccccgatt ttcgttttac cacgacgctt gaaaccaaag
    10741 gcaatgtgga aaacggcgta cttaagggtg acttagtggc gcgatttggt gccgatccga
    10801 cgttaaaacg tcaggatatt cgcaatatgg tcgcgacttt gaaaaaatct ggcgtcaacc
    10861 aaatcgatgg caatgtgttg atagatacct ccattttcgc cagccacgat aaagcccccg
    10921 gctggccatg gaatgacatg acacaatgct ttagcgctcc gcctgccgcc gccatagttg
    10981 accgcaactg tttctccgtc tcgctctaca gtgcccaaaa acccggtgat atggccttta
    11041 tacgcgtggc atcttattac cccgttacga tgttcagcca ggtacgcacc ctcccccgtg
    11101 gttctgccga agcgcaatac tgcgaactgg atgtcgtacc aggcgacctg aaccgcttta
    11161 cgctgacggg atgcctgcca caacgttctg agccgctccc gttggctttt gccgtgcagg
    11221 atggagccag ttatgccggt gcaattctga aagatgagtt aaaacaggcg ggtatcacct
    11281 ggagcggaac actgctgcgc cagactcagg ttaacgaacc tggaacggta gttgccagta
    11341 aacagtcggc cccgctgcac gatctgctta agattatgct gaaaaagtcg gataacatga
    11401 tcgccgatac ggttttccgc atgataggcc atgcgcgctt caatgtacct ggtacatggc
    11461 gggccgggtc ggacgctgtg cgtcagatcc tgcgccagca agccggtgtc gatattggaa
    11521 acaccattat tgccgatggt tcagggcttt cgcggcataa cctgattgcc cccgccacca
    11581 tgatgcaggt gctgcaatac attgcccaac acgacaatga acttaacttt atctccatgc
    11641 tgccgctggc gggctatgac ggctctttgc agtaccgtgc aggtctgcat caggcgggcg
    11701 tggatggaaa agtctcagcg aaaaccggtt cgttgcaggg ggtatataac ctggcgggat
    11761 tcattaccac agcgagcggg caacgaatgg cgtttgtgca atatctttct ggctatgcag
    11821 tagaacctgc ggatcagcgt aatcgccgta ttccgttagt gcgttttgaa agccgtttgt
    11881 ataaagatat ttatcagaac aattagtcaa aaagaaaccc cggcacatgg ccggggcttc
    11941 agattattga caaagtgcgc tttgtttatg ccggatgcgg cgtaaacgcc ttatccggcc
    12001 tacaaaatcg tgcaaattca acatattgca attctcttgt aggcctgata agcgtagcgc
    12061 atcaggctga tttggcgttt atcatcagtg attaacgctt gtaaatgaac tcaacgcctt
    12121 cttcgtcgtc ttcgtcccag tcgtcatccc agtcttcatc atcctcttca gcaatctctt
    12181 caagctgctg gcgatgataa tcgtcccaca tgaattcgac tttctctggc tgtttcgctt
    12241 cttcagcctg cacgaccggg ttttcaagga taaaggtcat cacatcccag cagagatctt
    12301 tcacgcccag tccgctcgcc gcagagatca gataatattt atcttcccag cccagcgctt
    12361 cagcaatcgc tttcgctttc tcttcagctt ctgccttatc cagcagatcg atcttattga
    12421 acactaacca acgcggtttc gccgccagat cctggctgta tttttccagc tcgctgataa
    12481 taatacgcgc gttttcaacc ggatcggtgc cgtcaatcgg atcgatatcg atgaggtgca
    12541 acaggacgcg gcaacgttcc aggtgcttca ggaagcggat acccagacct gcgccttccg
    12601 cagcgccttc aatcagtcct ggaatatcgg caacaacgaa gctcttttcg ttgtccattc
    12661 gtaccacacc cagacttggc accagagtgg taaacggata atccgccact ttcggtttag
    12721 ccgccgatac cgcgcgaata aaggtcgatt tacccgcgtt tggcatcccc aacataccga
    12781 cgtcagccag cagcatcagc tccagcagca gctcacgctt atcacccggc gtaccgttgg
    12841 ttttctgccg tggtgtacgg ttaacggacg atttgaaacg ggtattgccc agaccgtgcc
    12901 agccgccctt agcaaccagc agacgctgac cgtgtttagt catatcgccc atggtttcac
    12961 cagtaccctg gtcgattaca cgcgtaccta ccggcacttt aatcgtcacg tctttaccgc
    13021 gcttaccggt acaatcgcgg cttgcgccgt tctgaccgcg ctctgcacgg aaagattttt
    13081 caaaacgata atcgataagc gtgttcaggt tttcgtcggc ttccatccat acgtcaccac
    13141 catcaccacc gtcgccgcca tccgggccgc ctttcggaat atacttttcg cggcggaagc
    13201 tcacgcaacc attaccgcca tcacctgcaa cgaccagaat cgatgcttca tcaacaaact
    13261 tcattttatt ctccataaat cattcgcctg cgcggggttg cgataccacc gttgtatgct
    13321 tacgtaatcc gccccaaata cgatgaccaa tggcggaata catcgcgccc gcaaccacga
    13381 caaacgcacc gagataacct aaaaggttta acatcggtct ggcgaagaaa tcgggccagg
    13441 ccagtgataa aagatctgaa aaaaacagcg taaacagtgg ggtgagcgtg atgatcgcgc
    13501 tcacctgcgc tgcctgccag cgagccatcg cttccgccag ggcgccatat cctaccaatg
    13561 tattcagtcc gcaaaaaatt aaacatgcga gctgccagtg gctaagctgc gctatcactc
    13621 caggctttgc cagagggaag agcgcaattg tacataaagt gtacagtaaa aacaggatct
    13681 gcggtgaggc cagccgacgc aataaaacct tttgcgccac gccataactc acccaaaccg
    13741 tcgccgcacc gaccccaaag ataactcccc aggtgtaatc ggtgagcttt gtaaatatct
    13801 cgaccagact ggtgttaaaa aacatcacca ggccgctcag gagcatcaat gcccctacaa
    13861 cctgagtgct gcgcattttc tctttcagga taaatacgct ggcaaccatc atgccaactg
    13921 gcgagagttg cccaatcacc tgcgaagcgg tcggactcag gtattgcaag gatgagctga
    13981 acaggatgaa gttcccaaac agcccggcgg tcgccactgc caacaaaatc aaccagcgtg
    14041 gcttacgaaa cacgcgtaat ggcggcaacc tcttcttcac cgcaagaatg gcaccaaggc
    14101 caatactcgc catcaagaaa cggtaaaaca cgattgtcgg aggttccatc acctccagca
    14161 cctgcttcat tgcgattggc aacgcccccc agcaaattgc tgtggtgagc gccaaaagaa
    14221 tgccaatgcc tgcctgctgc ttcataccgt attccctgca aagcgcattt gcggattaac
    14281 gtcgcaccat gcgcattttt accggttatc gaatgtaaaa agccccgcaa cgtgttgcgg
    14341 ggctttcatc cgttaccggg acgcgaaaaa cttattcagc ttcgatgctg ataaatttac
    14401 ggtttttcgg gcctttaact tcgaatttca ctttaccgtc tgctttagca aacagagtgt
    14461 ggtcacgacc gcaacctacg ttagcgccag cgtggaattt ggtaccacgt tgacgaacga
    14521 tgatgctacc cgccagaacg gattcgccac cgaaacgctt aacgcccagg cgtttagctt
    14581 ctgaatcgcg accgttacgt gtggagccgc cagccttttt atgtgccatt tgaaatctct
    14641 cctcaggtct taggcgctga tgccagtaat tttcacatca gtgaaccact gacgatggcc
    14701 ctgctgctta cgatagtgtt tacgacgacg aaacttaacg attttaactt tctcgccacg
    14761 accgtgagca acaacttcag ctttgattac gccgccatca acgaaaggaa cgccgatttt
    14821 gacttcttca ccgtttgcga tcatcagcac ttcagcgaac tcaacagttt cgccagttgc
    14881 gatgtccagc ttttccaggc gaacggtctg accttcgctt actcggtgtt gtttaccacc
    14941 actttggaaa accgcgtaca tataaaactc cgcttccgcg cacacctttt caatgattca
    15001 gagtgcgcta taaatattca caatagggcg cgaatattac gcaaaacgca cgcctttgac
    15061 aagtgctaca gtcaatacac gaagaaaaaa aacacaactt gtacggtaac gtttatctgt
    15121 gccatttttt cagtacaatc accctatatt cctaaccata aaccctaagt tgcctttgtt
    15181 cacagtaagg taatcggggc gaaaagcccg gcttttgcga tgaatttaga aaaaatcaat
    15241 gagttaaccg cgcaagatat ggcgggtgtt aatgcggcaa tccttgagca gcttaattcc
    15301 gacgtccaac tgatcaatca gttaggctat tacatcgtca gcggcggcgg taaacgtatt
    15361 cgtccgatga ttgctgtact ggctgcacga gctgttggct atgagggaaa tgcgcatgtc
    15421 accattgctg ccctgatcga gtttatccac acggcgactc tgctacacga cgacgttgtg
    15481 gatgaatcag atatgcgcag gggtaaagct accgccaacg ccgcatttgg caatgccgcc
    15541 agcgtgctgg taggcgattt tatttatacc cgcgcgttcc agatgatgac cagcctcggt
    15601 tcactcaaag tgctggaagt catgtcagaa gctgtaaacg tcatcgcaga aggtgaagtt
    15661 ctgcaactga tgaacgttaa cgatccggac atcactgaag aaaactacat gcgcgttatc
    15721 tatagcaaaa ccgcgcgtct gtttgaggct gccgcgcagt gttccgggat tctggctggc
    15781 tgtacgccgg aggaggagaa aggcctgcag gattatgggc gctatctcgg cactgctttc
    15841 cagttgatcg acgatttact cgattacaat gccgatggcg aacagttagg taaaaatgtc
    15901 ggcgacgatc tgaacgaagg taaaccgacg ctgccgctgc tgcatgcgat gcatcatggc
    15961 acaccagaac aggcacagat gatccgtacc gccatcgaac agggtaacgg tcgccatctt
    16021 ctggaaccgg ttctggaagc aatgaacgct tgtggatctc ttgaatggac gcgtcagcgt
    16081 gccgaggaag aagcagacaa agccatcgca gcattacagg tgctcccgga caccccttgg
    16141 cgagaagcac tcatcggcct cgcgcacatc gctgttcaac gcgatcgtta atcccctccc
    16201 ttcatcccgc gcagtgcgcg ggatgattcc agtaaattcc ataaaaacac taattcagcg
    16261 ctataacacc tgaaaaaaag ggcgatgaat ctttatatat tctgaatatt cacactcttt
    16321 acaggaactt tttagagcaa taggccatca ggagtatagt gatgcccgac agaagaagtg
    16381 ttctgaatga aagcgactaa cttaaggagt gaggaaagat ggaaagtaat ttcattgact
    16441 ggcatcccgc tgacatcatt gcgggtttgc gcaagaaggg aacgtcaatg gcggcggagt
    16501 ctcgcagaaa tggtttgagt tcctcaacgc tggcaaacgc attatcgcgc ccatggccga
    16561 aaggagagat gattattgcg aaagccctgg gaactgaccc ctgggttatc tggccatcac
    16621 gctaccatga tccgcagacc catgagttta tcgacagaac gcagttgatg cgtagctaca
    16681 ctaaaccgaa aaaatgagtg gtctggcggt agccccgcga acggggctgc cagctctcag
    16741 acgattactc gcctttcaca cgctcaatat ttgcacctaa agcgcgcagt ttgtcttcaa
    16801 tgcgttcgta gccacgatcg atgtgataaa tacgatcaac caccgtcgtc ccttccgcaa
    16861 tacagccagc cagcaccagg cttgctgatg cacgcagatc ggttgccata acctgtgcgc
    16921 cagaaagttt ttcaacaccg tgacaaataa cggtattgct ttcgatttcg gcatgcgcgc
    16981 ccatacggct aagctccggc acatgcataa agcggttttc aaagaccgtt tcggtgatga
    17041 atccggtccc ttctgccacc aggttcaaca gcgtgaactg ggcctgcata tcggtcggga
    17101 atgccggatg cggcgcggta cgtacgttaa cagccttcgg acgtttgcca tgcatatcca
    17161 ggctaatcca gtcttcgccg acttcgatgt ccgctccagc gtcacgcagt ttcgccagca
    17221 cggcgtcgag agtatctggc tgcgcgttac ggcagataat tttgccgcga gaaatcgccg
    17281 ccgccaccag gaaagtaccg gtttcgatac gatccggtag cacgcgatag acaccgccgc
    17341 ctaaacgttc cacaccttcg atgacgatac gatcggtgcc ctgaccgcta attttcgcac
    17401 ccagcgtaat caggaagttc gcggtatcga cgatttccgg ttcacgcgct gcgttttcaa
    17461 taatcgtggt accttccgcc agggttgcag cacacatgat ggtcaccgtt gcgccaacgc
    17521 tgactttatc catcacgata tgtgcgcctt tcagacgacc atcgacggaa gctttaacgt
    17581 aaccttcttc cagtttgatg gtcgcgccta attgttcgag gccagaaatg tgtagatcaa
    17641 ccggacgcgc accgatcgta caaccgccag gcagtgaaac ttgcccctga ccaaagcgcg
    17701 ctaccagcgg ccccagtgcc cagatagaag cacgcatggt tttaaccaga tcgtaaggtg
    17761 cgcagaatac attaacgtcg cgggcatcaa tatgcacaga accattacgt tctactttcg
    17821 cacccagctg gcttagcagc ttcattgatg tatcgacgtc tttcagtttt gggacgttct
    17881 ggatctctac cggttcttcc gccagtagag cggcaaaaag gataggcaga gcagcatttt
    17941 tagcgccgga aattgtgact tcgccctgga gcttcgttgg cccctgaaca cgaaatttat
    18001 ccatttagtt tgttctcagt taacaattca tatccgctac cggcgaatcg cccatagctc
    18061 aaaagccgtt cagtttgcga tcgcgcgccc actccgcagg ggtatacgct ttgatcgaca
    18121 cagcatgaat gcggttatcc gcaatatatt ccatcagcgg gccatagacc gtctgctgtt
    18181 ttttaacccg actcatgccg tcaaacaact cacccacggc aataacctga aagtggctgc
    18241 catcgccgga aacgtggact tcctggaggg agagagcgtt catcaacacg ctctgaattt
    18301 cattattttc catgggatct tcaatcatca gttaataaac cagcgaaaca tcttagagca
    18361 aagttgcgct ggcataaata agcaaaaagc ctcgctgata aatcagacaa ggctcgactt
    18421 gcaggcaggt ttgccggaca ggcggttaac gccatatccg gcctgaaaaa atttaacgag
    18481 gcagaacatc agcaggcaaa ttatacaatt tcgcgagggt atacactttg tcgtttaccc
    18541 cctgaagcgt cacgttgttg ccctgctttt tcgccagatc gataagatgg agcagcagtg
    18601 ccagtccacc cgtatccacg cgggacacac ggctaagatc gatgcaggta atccccttca
    18661 ccgcttcctc acgcatttcc caaagcggta gcagaacgtc ctgatccagc tctccggata
    18721 acgccagcgt gtcacccgtc tgcatccagc tcagtgactc gctcattatt ttttctcttc
    18781 cagagtgatt ttctgttgag aaatcgattt cagttgcgca gtcaggccgt cgataccttt
    18841 ggtacgcagc agcgttcccc actcgttttg tttggtggtg atcatactga cgccttcagc
    18901 aatcatgtcg taagcctgcc agtttcccgt ctgggagttt ttacgccact ggaagtccag
    18961 acgcaccggc ggacggccat tcgggtcaat aatggtaacg cgaataggca caatggtttt
    19021 atcgcccagc ggctgttctg gcgcaatctg ataggtttga ccgtgataca tcgccagcgc
    19081 ctgaccgtaa gcctgcttca ggtactcacg gaaagcggca aagtaggctt cacgttgagc
    19141 aggggtcgca ctcttgtaat actggcccag caccagcgca ccggcgtatt tcacctgtac
    19201 gtatggcagc agttcctgat caacaatggt acgcagataa tccgggttgg cccgaatttg
    19261 cggttgctca ttcttcaggc gatcgaacgt tttctgcgcc gcctcgtcca tcagcttata
    19321 aggattggtc tggtctgcct cggttgccgc actcagaggt gcaatcacca gcaaagcgac
    19381 catcattaaa cgtttaaaca tgcgtcggtt ctcctgaaat tatttcgttg tacccacagg
    19441 ttcagtggtt tcattattac ctggcgcagc agctggcgca tcgccactat tcttattgtc
    19501 atcgccttta ctaccgtaaa ggaactgacc aatgagatct tccagcacca tcgcagactt
    19561 agtgtcctga attgtatcgc catccttcag gatagcagtc cccagttccg ggtcttcaaa
    19621 accgacgttt aatgccagat attgttcccc cagcaggccg gaagtacgaa tgctcagcga
    19681 actggtatct ggaatgtggt tataacgttg ttcaatttcc agcgttacgc gcggcagata
    19741 ggttttcggg tccagcgtaa tatccgccac ccgacccaca acaacgccac caatactgac
    19801 cggagagcgc gctttcaggc cgccaatgtt atcgaacgtc gcataaagcg tgtaggtcgg
    19861 ttcagtacgt atggacgtca cgttcgccgc cttcaggcaa acaaacagcg ccgccagcag
    19921 tgctgctaat aaaaagatac ccacccaaat ttcatttttt ttcgtttgca tgaactcaat
    19981 tcccaaacat caatgcggtc agcacaaaat ccagccccag aacagccaga gacgagtgga
    20041 caacggtgcg agtggttgcc cggctaatcc cggcagacgt cgggatggcg tcgtagccgt
    20101 taaacaacga aatccacgtc accgtgatgg cgaacaccac gctcttaatc agacagttga
    20161 ccagatccat acgccagtcg acggcatttt gcattgccga ccagaagaac ccgctatcaa
    20221 tgcctttcca actgacgccg accagagatc cgccccagat ccccacggcg acgaaaataa
    20281 ccgtcaacag tggtaatgaa ataaccccag cccagaaacg gggagaaata acccgacgca
    20341 gcggatccac cgccatcatc tccatactgg agagttgctc tgtagcgcgc atcaggccga
    20401 tttctgcggt tagcgccgaa ccagcacgcc cggcaaacaa caacgcggca accaccggcc
    20461 ccagttcacg cagtagcgat aacgccacca gcatacccag actggtttcc gcactataag
    20521 tggtcagaac cagataacct tgcagcccca acaccattcc gatgaacacg ccagaaacca
    20581 caataatcag catcgacagg acgccgacat tatagagctg gcgcaccagc agcggcgcat
    20641 gtttgcgaaa ttccggtttg ccgaccagcg cattgaataa cattaacccg gcccgcccga
    20701 acgttctcag ggttttaatc cctttatgtc cgagcgacgc cagcgcattt aacagcatga
    20761 gtggcttaac tccctggtaa aagatcagcg tgataatcgc cggcaggata gcggaacgga
    20821 acaggcccgt cagctatccc gtccagaaac tgacgtacgc gcggatcagg attcgcctgc
    20881 aacgcctggg cactgccatg agcgacaatt tttttgtccg ccaggatcca ggcgtgatcc
    20941 gcaatactta acacttccgg cacatcgtga gaaaccacca cacaagtcac gcccagcgcg
    21001 ctgttcagct cagaaatcag cttcaccagt acgcccatgg tgatgggatc ttgcccaaca
    21061 aaaggttcat caaacatgat gagatccggc tccagcgcaa tcgcacgcgc cagcgctgca
    21121 cgccgcgcca tcccaccgga aagttcagaa ggcattagtt tagccgctcc acgcagcccc
    21181 acggcctcca gcttcatcat caccgtacta tgcaacaatg gcgcgggtag ttgggtatgt
    21241 tcgcgcagtg gataggcgac gttgtcaaat acgttcatat cagtgaacaa cgccccggac
    21301 tgaaataaca tgctcatccg tttgcgcact gtatacaggc gcgaacgaga catcgccgga
    21361 atattctcac catcgaaaag gatctcacca tgatctggtg cgatttgccc gccaatcaga
    21421 cggagtagcg tcgttttacc gatgcccgat ggccccatga tcgccgtgat cttccctcgc
    21481 ggcacggtca gggaaatatt atcgaagatg cagcgattgc cacgcgtaaa actgacatcg
    21541 cgcatatcga ctaaattcgc cacagactgc tccataattc acccttcgtc ttgcgttgat
    21601 tttctaagca tggcgctcaa tttaaccttg aacccaacat atttacagaa tattacccgc
    21661 cgtggttagc gaaagctggc atttgtttta ctttttagcc gcataaagtc aaaattaaga
    21721 atccgttacg gctttctgaa aatcttcagc ggaccgacga gtatacctga agaaaggacg
    21781 ttagatgctt ttagctacgg cactgttaat tgttggttta cttttggtcg tttacagtgc
    21841 cgaccgcctg gtttttgccg cgtctattct ttgccgaacc tttggcatcc cgccgctgat
    21901 catcggcatg acggtggtca gtattggtac atcgttacca gaaatcatcg tctcgcttgc
    21961 cgcgtctctg cacgaacaac gcgatttagc cgttggtaca gccctcggct caaacattat
    22021 caatattttg ctgatcctcg gtctggctgc gctggttcgt ccttttaccg tccattccga
    22081 tgttctgcgt cgtgaattac ccttaatgtt gttggtcagc gtagtggccg gttccgtact
    22141 ctatgacgga caacttagtc gcagcgatgg tatctttctc ctctttctgg ctgtgctatg
    22201 gctgctgttc attgttaaac ttgcacgtca ggctgaacgt caggggactg acagcctgac
    22261 cagagagcag cttgcagagc tgccgcgtga cggcggattg cccgtcgcgt ttttatggct
    22321 cggcattgcg cttatcatca tgccagtggc cacgcggatg gtggttgata acgccacggt
    22381 gctggcgaat tactttgcca tcagcgagtt gacgatgggt ctgacggcaa ttgctatcgg
    22441 aaccagcctg ccggaactgg caaccgcaat agcgggggtt cgcaaaggtg aaaacgacat
    22501 tgctgtcgga aatatcattg gcgcaaacat ttttaatatt gtcatcgtgt tgggtttacc
    22561 cgcgctgata acgccaggag agattgagcc attggcgtac agtcgtgact acagcgtgat
    22621 gttgctggtg agcattattt ttgcgttgct gtgctggcgg cgctccccgc aaccgggccg
    22681 tggtgtaggg gtattattaa ctggcggatt tatcgtatgg ctggcgatgt tgtactggtt
    22741 atcgccaata ctcgttgaat aactggaaac gcattatgtc gcacgtagag ttacaaccgg
    22801 gttttgactt tcagcaagca ggtaaagaag tcctggcgat tgaacgtgaa tgcctggcgg
    22861 agcttgatca atacatcaat cagaatttca cgcttgcctg tgaaaagatg ttctggtgta
    22921 aagggaaagt tgtcgtcatg gggatgggga aatcggggca catcgggcgc aaaatggccg
    22981 caacgtttgc cagcaccggt acaccttcat ttttcgtcca tcctggtgaa gccgcgcatg
    23041 gtgatttagg catggtcacc ccacaggatg tggtgattgc tatctctaac tcaggtgaat
    23101 ccagcgaaat cacggcctta attccagtgc ttaagcgtct tcacgtaccg ttaatctgca
    23161 tcaccggtcg cccggagagc agcatggcgc gcgccgcaga tgtgcatctg tgtgttaaag
    23221 tagcgaaaga agcctgtccg ttagggctgg caccgaccag cagcaccacc gccacgctgg
    23281 ttatgggcga tgccctcgct gtcgcgctgt taaaagcacg cggctttact gctgaagatt
    23341 ttgcgctctc acacccaggc ggcgcactgg gtcgtaaact tctgctgcgc gtaaacgata
    23401 ttatgcatac gggcgatgag atcccgcatg ttaagaaaac ggccagtctg cgtgacgcgt
    23461 tgctggaagt tacccgcaaa aatcttggta tgactgtcat ttgcgatgac aatatgatga
    23521 ttgaaggcat ctttaccgac ggtgatttac gccgtgtctt cgatatgggc gtggatgttc
    23581 gtcagttaag tattgccgat gtgatgacgc cggggggaat acgtgtgcgc cctggcattc
    23641 tggccgttga ggcactgaac ttaatgcagt cccgccatat cacctccgtg atggttgccg
    23701 atggcgacca tttactcgga gtgttacata tgcatgattt actgcgtgca ggcgtagtgt
    23761 aaagattcaa ggataaacaa caatgagcaa agcaggtgcg tcgcttgcga cctgttacgg
    23821 ccctgtcagc gccgacgtta tagcaaaagc agagaacatt cgtctgctga tcctcgatgt
    23881 cgatggcgta ctgtcagatg gcctgattta tatgggcaat aatggcgaag agctgaaagc
    23941 gttcaatgtt cgtgacggtt atggcattcg ttgtgcgctc acctctgata ttgaagtcgc
    24001 tatcattacc gggcgaaagg ctaaactggt agaagatcgt tgtgccacat tggggatcac
    24061 tcacttgtat caggggcagt caaacaaact gatcgccttt agcgatctgc tggaaaaact
    24121 ggcgattgcc ccggaaaatg tggcttatgt cggcgatgat ctcatcgact ggccggtaat
    24181 ggaaaaagtg ggtttaagcg tcgccgtggc cgatgcgcat ccactgttga tcccgcgcgc
    24241 cgattacgtg acgcgcattg ctggcggtcg tggcgcagtg cgcgaagttt gcgacttatt
    24301 actcctggcg cagggcaaac tggatgaagc caaagggcaa tcgatatgag taaagccaga
    24361 cgttgggtta tcattgtgct atcactggcg gttctggtga tgatcggcat tgatatggcc
    24421 gaaaaagacg ataccgccca ggtggtcgtc aacaacaatg atcccaccta taaaagcgag
    24481 catacggaca cgctcgtcta taacccagaa ggggcactaa gctatcgatt gattgctcaa
    24541 cacgttgaat attattccga tcaggccgtt tcgtggttta cgcagccggt acttaccacg
    24601 tttgataagg ataaaatccc gacatggtcc gtaaaagcag ataaagccaa gctgaccaat
    24661 gaccggatgc tctatttata tggacacgtt gaagtcaacg cacttgtgcc agactctcaa
    24721 cttcgcagaa tcacgacgga taacgcgcag atcaatctgg tgacgcagga tgttacctct
    24781 gaagacctcg tcacgttata cggaacaaca tttaactcca gcggtctgaa aatgcgcggc
    24841 aacttacgca gcaagaacgc cgagctgatt gaaaaggtta gaacatccta tgaaattcaa
    24901 aacaaacaaa ctcagcctta atcttgtgct tgccagctca cttctggccg ccagcattcc
    24961 ggcatttgcc gtaaccggag acactgatca gccgatccac attgaatcgg accagcaatc
    25021 tcttgatatg caaggcaacg tggttacctt taccggtaat gtcatcgtca cccagggcac
    25081 catcaaaatt aatgccgaca aagtggtcgt tacccgtccg ggcggcgaac aaggtaaaga
    25141 agtgattgac ggctacggta aaccggcaac gttctaccag atgcaggaca acggtaaacc
    25201 cgttgaaggt cacgcttccc agatgcacta cgaactggca aaagattttg tcgttctgac
    25261 gggtaatgct tatctgcagc aggtcgatag caacattaag ggcgataaga tcacttacct
    25321 ggtaaaagag cagaaaatgc aggctttcag cgacaaaggc aagcgcgtaa ccaccgttct
    25381 ggtgccgtcg cagctgcagg acaaaaacaa caaaggccag accccggcac agaagaaggg
    25441 taattaattc gttatggcaa cattaactgc aaagaacctt gcaaaagcct ataaaggccg
    25501 tcgcgtggta gaagacgtca gcctgaccgt caactccggg gaaattgtcg gtctgctggg
    25561 gccaaacggt gccggtaaga ccaccacttt ctacatggtt gtaggcattg tgccgcgcga
    25621 tgcgggcaac atcattattg atgatgacga tatcagtctg ctgcctctgc atgcacgcgc
    25681 gcgccgcggt atcggctatc tgccacagga agcctccatt ttccgtcgcc tcagcgttta
    25741 cgataacctg atggcggtac tgcaaattcg tgacgacttg tctgctgaac aacgtgaaga
    25801 ccgcgcgaac gagctgatgg aagagtttca cattgagcac ctgcgtgaca gcatggggca
    25861 gtcactctcc gggggtgaac gtcgccgtgt agaaattgcc cgcgcactgg ctgcgaatcc
    25921 gaaatttatt ctgctcgacg aaccgtttgc cggggttgac ccgatctcgg ttatcgacat
    25981 taaacgcatc attgagcacc tgcgcgacag cggcctgggc gtgctgatca ctgaccacaa
    26041 cgtgcgtgaa acactggcgg tttgtgaacg cgcttatatc gtcagtcagg ggcatttgat
    26101 cgcccacggc acgcctacag aaatcttaca agacgaacac gttaagcgtg tataccttgg
    26161 ggaagacttc agactctgat agggtagaag tttgcgacgt tttagcagga gagtacgatt
    26221 ctgaacatga agcaaggttt gcaactcagg cttagccaac aactggcgat gacgccacag
    26281 ctccaacagg caattcgtct gttgcagttg tcgacgctgg aacttcagca ggagctacag
    26341 caggcgctgg agagtaatcc gctgcttgag caaatcgaca ctcatgaaga aatcgacacc
    26401 cgcgaaacgc aagacagtga aacgctggac accgccgacg cgctcgaaca aaaagagatg
    26461 ccggaagagc tgccgctcga tgccagttgg gacaccattt acaccgctgg tacaccatcc
    26521 ggcaccagcg gtgactacat tgacgatgag ctgccggtct atcagggcga aacgacgcag
    26581 accttgcagg attacctgat gtggcaggtc gagctgacac cgttttccga cactgaccgc
    26641 gctattgcta cctctatcgt cgatgccgtt gatgacatcg gttatctgac tgtcccgctg
    26701 gaagatattc tcgaaagtat gggcgatgaa gagatcgaca tcgacgaggt tgaagccgtc
    26761 cttaagcgga tccaacggtt tgatccggtc ggtgtagcgg caaaagatct gcgtgactgc
    26821 ctgctaatcc aactctccca attcgataaa accacgccgt ggctggaaga ggccagactg
    26881 atcattagcg atcatctcga tctgttagcc aatcacgact tccgcacttt aatgcgcgtc
    26941 acgcgtctga aagaagatgt gctgaaagaa gccgtcaatc tgatccagtc gctcgatccg
    27001 cgccccgggc agtcgatcca gactggcgaa cctgagtatg tcattccaga tgtgctggtg
    27061 cgtaagcata acggtcactg gacggtagaa ctcaacagtg acagcattcc gcgtctgcaa
    27121 atcaaccagc actacgcctc gatgtgcaat aacgcgcgca acgatggtga cagccagttt
    27181 atccgcagca atctgcagga tgccaaatgg ttgatcaaga gtctggaaag ccgtaacgat
    27241 acgctactgc gcgtgagtcg ctgtatcgtt gaacagcagc aagccttctt tgagcaaggt
    27301 gaagaatata tgaaaccgat ggtactggcc gatatcgccc aggctgtcga aatgcatgaa
    27361 tcgacgatat ctcgcgtgac cacgcaaaaa tacctgcata gtccacgagg catttttgaa
    27421 ctgaagtatt tcttttccag tcacatcaat accgagggcg gcggcgaagc ttcctccacg
    27481 gcgattcgtg cgctggtgaa gaaattaatc gcggcggaaa acccagcgaa accgttgagc
    27541 gacagcaagt taacctcttt gctgtcggaa caaggtatca tggtggcacg ccgcactgtt
    27601 gcgaagtacc gagagtcttt atccattccg ccgtcaaacc agcgtaaaca gctcgtttga
    27661 cccaaccgat aaggaagaca ctatgcagct caacattacc ggaaataacg tcgagatcac
    27721 cgaggcactg cgcgaatttg ttacagccaa atttgccaaa cttgagcaat attttgaccg
    27781 aatcaaccag gtctatgttg ttctgaaagt ggagaaagtc acccacacct cagatgcaac
    27841 actgcatgta aacggtggtg aaattcatgc cagcgcggaa ggtcaggaca tgtacgctgc
    27901 cattgatggc ttaattgata agctggcacg ccagttgacc aaacataaag ataaactgaa
    27961 acaacactaa ttgtccgggc aattagcatg tgcatggcgg cctgttgtgc ggcacaacgg
    28021 gccatttgta cggttaatgc tccgagcctg ttccactgtt tgagtgggca ggttcttagg
    28081 tgaaattatg acaaataatg atacaactct acagcttagc agtgttctta acagggaatg
    28141 tacgcgaagc cgcgtccact gtcagagcaa aaaacgcgcc ctggaaatca tcagcgagct
    28201 ggcggcgaaa caacttagcc tgccgcctca ggtggttttt gaagctatcc tgacgcggga
    28261 aaaaatgggc agtaccggta tcggcaatgg tattgccatt ccgcatggca aactggaaga
    28321 agatactctg cgcgccgttg gcgttttcgt tcagctcgaa acgcctatag ctttcgatgc
    28381 catcgacaac caaccggtgg acttactttt tgccctgctg gtgtcggcag accaaactaa
    28441 aacgcacctg catactctgt cgctggtggc gaaacgtctg gcggacaaaa ccatctgccg
    28501 ccgtttacgc gcagcccaga gcgatgaaga gctgtatcaa atcattacgg ataccgaagg
    28561 tactccggat gaagcgtagt tattcggtaa tgtctctttt agacgttgtg aggagaaaca
    28621 gtacatggta ctgatgatcg tcagcggacg ttcaggttca ggtaaatctg tcgccctgcg
    28681 tgcgctggaa gatatgggtt tttactgcgt ggataacctt cccgtagtgt tgttacccga
    28741 tctggctcga accctggccg atcgcgagat ttctgccgcc gtcagcattg atgttcgtaa
    28801 tatgccggag tcaccagaaa tattcgaaca ggcgatgagt aacctgcctg acgctttctc
    28861 accgcaactc ctgttcctgg atgccgaccg taatacctta attcgtcgtt acagtgacac
    28921 gcgccgactg catccgcttt ccagcaaaaa cctgtcgctg gaaagtgcta tcgacaaaga
    28981 aagcgatttg ctggagcctc tgcgttcgcg agcggatctg attgtcgata cctcagaaat
    29041 gtccgttcac gagctggctg aaatgctgcg tacccgtctg ctgggtaaac gtgaacgcga
    29101 actgaccatg gtctttgagt ctttcggctt caaacacggt atccctatcg atgcagatta
    29161 cgtctttgac gtgcgcttct tgccgaaccc gcactgggat ccgaaactgc gtccaatgac
    29221 aggtcttgat aaacctgtcg ccgcgttcct cgaccgccac acagaagtac acaattttat
    29281 ctaccagacg cgaagctatc ttgagctatg gttacctatg ctggaaacca acaaccgtag
    29341 ctacttgacg gtcgccattg gttgtaccgg cgggaagcac cgttcggtgt atattgcaga
    29401 gcaactggca gactacttcc gctcgcgcgg taaaaacgtc cagtcacgcc atcgtacgct
    29461 ggaaaaacgt aaaccatgac cgtcaagcaa actgttgaaa tcacaaacaa gctgggcatg
    29521 catgcccggc ctgcaatgaa gctgtttgaa ttaatgcagg gttttgacgc tgaagtgctg
    29581 ttacgtaatg acgaaggcac cgaggccgaa gccaacagcg ttattgcgct gctgatgttg
    29641 gattctgcca aaggacggca gattgaagtt gaagcgaccg gcccacagga agaggaagca
    29701 ctggccgccg ttatcgccct ctttaactct ggttttgatg aagattaatc ttcatcactt
    29761 tgacatacaa ctcccttcaa actccccccg acaataagaa aatcacgtac tgaaatcgtt
    29821 ctcaatcaac gtcatttgta cattttgtgc gcttttcact ttcagaagaa ccttaagaaa
    29881 accttaagag gcattgttta ggttttgttt aagttaatcg accatactgg agatcgtcag
    29941 aaaatatttc caggagatgg catgattcgc ttatctgaac aaagtcccct gggcaccgga
    30001 agacatcgga aatgttatgc gcatccggaa gatgcccaac gctgtatcaa gattgtctac
    30061 catcgtggcg atggtggcga taaagagatc cgccgtgagt taaagtacta cgcgcatctt
    30121 ggtcgccggt taaaagactg gagtggaata ccgcgctatc acggtaccgt tgagacggat
    30181 tgcggaaccg gttatgtcta cgatgtgatt gcagattttg acggcaaact ctccattacg
    30241 ctgaccgaat ttgctgaaca atgccgttac gaagaagaca ttgcacaact gcgccagcta
    30301 ctgaaacagc tgaagcgtta tttgcaggat aaccgtatcg tgacgatgtc gctgaagccg
    30361 cagaatatcc tctgtcaccg catcagtgag tcagaggtta tcccggtcgt ctgcgacaac
    30421 attggtgaaa gcacattgat tccactggcg acctggtcga agtggtgttg tctgcgtaag
    30481 caagagcggt tatggaaacg atttatcgcc caaccggcgc tggctattgc cctgcagaaa
    30541 gatctgcaac ctcgtgagag caaaacgctg gccctcactt cgcgcgaagc ttaatccagc
    30601 tggtgttgtt gcataaacgg ctcaccgcct aactgataca tctgccgtaa aatccacgcc
    30661 tgacggctac gcacgtagcc cgatggcgag gagactttga aacgaagtgg attaggtaat
    30721 acagctgcca gtaatgcagc ttccgaccgg gtaagtttgc tcgcgggttt gtggaaataa
    30781 cgttgtgccg cagcttcgac gccaaacaca ccgtcgccaa attcggcgat attcaggtaa
    30841 accgtcagga tacgcttttt gctccagact gtttctatcc ccagcgttaa tccagcttcc
    30901 agcccttttc gtacccagct acgcccatcc cataaaaaga gatttttggc cgtctgctga
    30961 gaaatcgttg aagcaccgcg aatacggttt tcattgcgct cgttgtgcgc cagggctttc
    31021 tcaatggaag cgacatcaaa gccccagtgc tcaggaaatt tctgatcttc tgcggcaatc
    31081 accgccagtc ccatccacgg cgagatttga tccatactga cccagtcaga atgtgccacg
    31141 taacgaaaat tgccatgcag ccaggcgctg acctgtcgct cgaccattac cgctgagaag
    31201 ggaacaggcg caacactaaa caacgcgatg cccccgcccc agaaaacggc gaggacaacc
    31261 attaacagca aaagaaaacg gcgaacgaaa ctaaacaccg ttaagcggct tttactcatt
    31321 cagccagaac cagcacgcgg gaaaccagct tatcaatgcc gctcgccgct tctgcaatgt
    31381 tctgcgccag catatatgct ggggtggtga caatcttatt gtcttcatca accacgatat
    31441 catcgacagg acagggcaca tgctccgcgc ccatctcttc cagcacttct gcggtatcga
    31501 tatcagtacc gatggtcaaa cgcagcggga aatcgaaaat tttcggcagc atcgccgggg
    31561 caatacacat aaaaccaagc ggttttccgg cctgatgcat cgcttgtgcc agcgccttta
    31621 attcacggtc aacggtgcat tcgctaccaa gagtggcaaa attgcttaaa ttcttcgccg
    31681 cgccaaaccc ccccggcaca atcaacgcat ccagttcagc ggcatcggcc tgggccagag
    31741 gacggatttc accgcgcgtt attcgtgccg cttcaatcag cacgttgcgc gtttccgtca
    31801 tcgcttcgcc agttaaatgg ttgataacat caacctgctg cttatccggt gcaaagcaga
    31861 ccgcctgcgc accgctgcgt gaaatagcta acaacgtcaa caccgcttca tgaatttcag
    31921 aaccgtcata gacgccgcat ccgctcagaa ttacgccaat tttcttcatt gtgatcatcc
    31981 tttcgcaatt gactgaaaca cattaatttt tttaataaaa atggtacgca tcacacattt
    32041 aactgattca tgtaacaaat catttaagtt ttgctatctt aactgcgtgc ggcctgaaaa
    32101 acagtgctgt gcccttgtaa ctcatcataa taatttacgg cgcagccaag atttccctgg
    32161 tgttggcgca gtattcgcgc accccggtct agccggggtc attttttagt ggcttttgcc
    32221 acccacgctt tcagcacttc tacgtcgtga cgccactctt ctttcatctc ttcaatccat
    32281 tcaccgacgt tatcttccca ggcaggaagg tcaggagact gaatttgctg acccagctgt
    32341 tgcagatggc gtaaccccac tgaccccgcc gcacctttaa ttttatgtcc ttcctcaaca
    32401 atgccttttt tatcctgcgc cgtcagattc gactccagca cgctgacata gcccggcatc
    32461 atcttctcaa acaccgctaa cccgtcggtg atcagcttcg gtcctacaag ttcgagatac
    32521 tgttccagca tgggaatatc gagcaatgct tctgatttac tgttctcttc tgtcgtcacc
    32581 gtactctcct catcatcctg ggtatcccag aatttcttga tcatcgcggt tagcgccgga
    32641 acagaaagcg gcttactcag cacatcatcc attccagcat tgaggtactc ttgtttgtct
    32701 ttcagcacgt tagcggttaa ggccaccagc ggcggtaaat cctctcgcgg ataacgcttc
    32761 gtcagttcac gagagatatc cagcccggtc atatctggca actgaatatc cagtaacacc
    32821 aggtcgtatt cgcccggttt aaacatctct agcgccgcct tgccggtcat ggcgacatca
    32881 acgctgttac ctaatttttc cagcacagaa cgcgcaacaa tcacgttcag ttcaatgtct
    32941 tccaccagca gcacattcag cgccggtaaa ggcatatcgt cttcatcaaa cgcatcatcg
    33001 acctcttctg ctaccgacgg tgcgtggatc gtcaacgtaa aggttgaacc tttgccctgt
    33061 tcgctggtaa ccgtaatatc gccgcccata tttttcgcca gacgacgaga aacggccaga
    33121 ccaataccgg tgccggtggc aggtttaccg ccatgactgt ctttcacctg gtaatacatg
    33181 gcgaaaattt tatccagctc atcctgcgga atgccgatac cagagtcttc cacttcaaaa
    33241 tgcagcatat cgccttcatc gtagcgcacg cgcacggtaa cctggccttg ctgggtgaat
    33301 ttgacggcgt tactgatgag gttccacagg atctgccgta aacgcgtccc gtcggtaatg
    33361 acctgatgcg gtaatggcag cgtcggctcc aggttaaagc gcaatccttt ttgttgcgcc
    33421 tgcaatgcgg agagattttc cagatcggca aggaagctgg tgaaatcaac cggttgatta
    33481 tcaagctgga ccttgcgccg ttccatctta tccatgtcga taatatcgtt aaagatattc
    33541 cccagcgtga cggccgaaac atggatggtc ttgagatatt tttcctgctc ggcggtgagt
    33601 tcggtatcca gcagaatgcg gctcagaccg acgataccgt tcagcggtgt acgcaattcg
    33661 tgactgatgg tggagataaa cgtcgttttg tcgcggctgg cccgttcaag cgcatcctga
    33721 taccgcttac gctcggtaat gtctcgacca aagcccatca aaccgtgacg tttacccacg
    33781 cggtcgtagt acggcacttt acggatttca aagcaggctt tgcgcccgtc cgggtaatcc
    33841 agccactgtt cataggtcag tgacacatta tgacggaaca ctttttcatc ggtttcaatg
    33901 acttttgcgg cggcttccgg tgagtaaaca tcagcaggtt tcaggtgaac cagttgtttt
    33961 tcgctttttc cggtcagcag ctccatcgcg cggttacagc cggaaaactc tttatcttcg
    34021 ttacgataaa aaaccaggtc gggtgaagca tcaaggaagg aacgtaagaa tgaggattgc
    34081 tgctcgagct gaatttgtgt ctcttcgcgc tctttgattt caattttcag ttggccgaag
    34141 gtttcctgca gttctgcttc cgctttttca cgaacggcga tttcctgatt tagctgggca
    34201 atattatctt ttaactgaac gttgaggctc aaatcgcgct cgcgcatctc ctccagtttt
    34261 tgcaccagcc gtgacagacg ttgtcgtgac tcctccagtt gctcgacgac caccgataga
    34321 aagtagaccg cccacggcgt aatcaacaaa ccaaagaaga tagaacgaat aacatcaatg
    34381 ctttcgacct gaccatgcag caccatggtt accgccattt gtaccacaat ggcaagaacg
    34441 acgagggcca gcgccagcaa cattgagaag cgcaccagac ctaacttcat catcaggtca
    34501 acataatact gcgccagcag acgaatttgc ttcattaggg aattccttca cgacaacctg
    34561 ccccaataat acccaattct gacagttacg ttataaattg tgtgagaaat gcggaattcc
    34621 cctcaacgac ctactccgtt ggaggtagcc acggacgtcc cagcgccgat ccctgtacgc
    34681 catgttcatt cagatagcga tccagctcga ccattcccgt ccatcgattt tcgcaccaca
    34741 gaggagcaag cagcgtcggg cgacgggcgc tggcggaaat gcggtggtag atcacttccg
    34801 gtggcgtatg gcgaatcatt tctccggcag tgagcgtgta atcctccagt tcaataccgt
    34861 tcaaacgccc cgcttcccag gctttcgcca taatgctgcc tttcacaata tgcaacggat
    34921 gtagctttat accgtcaaca ccggtttcca ctacccgctc cagcgtttgc agacattcag
    34981 ccagcccttc gcctggcaga ccgacaatta aatgcgagca caccttcagc ccgcgctggc
    35041 gtgccagctg cgtcgtgcgc tgataacagg cgaaatcatg accgcgatta atgcgatgta
    35101 gcgttttgtc gtgcacggtt tgcagcccca gctccagcca cacttcgtag ccctggtctt
    35161 tatattcgca aagcagatcc agcaccgcat ccggcacgca gtccgggcgc gtgccgacac
    35221 acaaaccgac aatattggcc tggctcaccg cctgttgata catcgaacgc agcacctgaa
    35281 cttcggcaaa ggtgctggta tacgcctgga aataggcgag gtaacgtttc gcgcgattaa
    35341 ttaaattcgc ctgatgcgcc agctgttcgg caatggaacg atgctgctgc gcttcgtcgg
    35401 caaacgaggc aacattacag aaggtgcagc cgccgcgccc gatggtgccg tcgcgattcg
    35461 ggcaactgaa accgccgtgc agcgtcagct tatgcacctt ttgcccataa cgacgggtga
    35521 gatcaccacc aaacatattg actaatttct gtaactgcat aatctgatag accgcgcctt
    35581 gaaaagaggc caaagcctgc catttttagc tcaattcggc gatgacctgg atcaatcgtc
    35641 ccggcccgct tttatcaact gcataatcaa tcaaaattac cgaaatttca tgcataatca
    35701 cataaatcac ttttgcttat cttgtgtcag atttttttat ctccttatgg attttaggca
    35761 aaaacagtag catgaaacgt cattaccaat taatgcagta taaaatgctg gttttctcgt
    35821 caattcaagg caggataagg gttaacacgc ctttatgaca gtcaggaatt gactgtttct
    35881 ctaacgactt cccttttagc cttaaagata aaatctattt taatttcagc catttaataa
    35941 agaattttgc gctaaagcac atttctgtac caataagctt gccatttgac ctgtatcagc
    36001 tttcccgata agttggaaat ccgctggaag ctttctggat gagcagcctg ctcatcatat
    36061 ttatgcagta attgagatcc cctcttcacc gtattaaccg atgcgaaaag gacaacaagg
    36121 gggcgaatgc gaggcgcgcg tatgacacgc aacccccgtc gccacgctct ttctgtaccc
    36181 gtgcgcagcg gttcggtagt ggggttcccg cagagcctgg gggaggttca cgatatgttg
    36241 tacgataaat cccttgagag ggataactgt ggtttcggcc tgatcgccca catagaaggc
    36301 gaacctagcc acaaggtagt gcgtactgca atacacgcac tggcccgcat gcagcaccgt
    36361 ggcgcgattc tcgccgatgg taaaaccggt gacggttgcg gcttgctgtt acaaaaaccg
    36421 gatcgctttt ttcgcatcgt tgcgcaggag cgcggctggc gtttagcgaa aaactacgcc
    36481 gtcgggatgc tattcctgaa taaagatcct gaactcgccg ctgccgcacg ccgcatcgtt
    36541 gaagaagaac tgcaacgcga aaccttgtcg attgtgggct ggcgtgatgt ccccactaac
    36601 gaaggcgtac tgggtgaaat cgccctctcc tctctgcccc gcattgagca aatttttgtg
    36661 aacgccccgg caggctggcg tccgcgcgat atggagcgcc gtctgtttat cgcccgccgc
    36721 cgcattgaaa agcgtctcga agccgacaaa gacttctacg tctgtagcct atcgaatctg
    36781 gtgaacatct ataaaggtct gtgtatgccg gcggatctgc cgcgctttta tctggatctt
    36841 gcggacctgc gtctggaatc ggccatttgc ctgttccacc agcgcttctc cactaacacc
    36901 gtaccgcgct ggccgctggc gcaaccgttc cgctatctgg cgcataacgg tgaaatcaac
    36961 accatcactg gtaaccgcca atgggcgcgt gcgcgtacct ataaattcca gacgccgctt
    37021 atccctgatc tgcacgacgc cgcaccgttc gtcaacgaaa ccggctctga ctccagttcg
    37081 atggataaca tgctggaact cctgctggca ggcgggatgg atatcatccg cgccatgcgt
    37141 ctgttagtgc cacccgcctg gcagaacaac ccggatatgg acccggaact gcgtgccttc
    37201 tttgacttta actccatgca tatggagccg tgggatggcc cggcgggcat cgtgatgtcc
    37261 gacggtcgtt ttgccgcctg taacctcgac cgtaacggtc tgcgtccggc gcgctacgtc
    37321 atcaccaaag ataagctcat cacctgcgcc tctgaagtcg gtatctggga ttaccagcct
    37381 gacgaagtgg tcgaaaaagg ccgcgtcggg ccaggcgaac tgatggttat cgacacccgc
    37441 agtgggcata ttctgcactc ggcagaaacc gatgacgatc tgaaaagccg ccatccatat
    37501 aaagagtgga tggagaaaaa cgtccgccga ctggtaccgt ttgaagatct gcccgatgaa
    37561 gaagtgggta gccgcgaact ggacgacgac acgcttgcca gctaccagaa acagtttaac
    37621 tacagcgcgg aagagctgga ctccgtaatt cgcgtactgg gcgaaaacgg tcaggaagcg
    37681 gtcggttcga tgggcgatga taccccattc gccgtgctct ccagtcagcc gcgcattatt
    37741 tacgactact tctgccagca gtttgcccag gtgactaacc cgccaatcga cccgctgcgt
    37801 gaagcgcatg ttatgtcgct cgccaccagt atcggtcgtg aaatgaacgt cttttgcgaa
    37861 gcagagggcc aggcgcaccg tttaagcttt aaatcgccga ttctgctcta ctccgatttc
    37921 aaacagctca cgacgatgaa agaggagcac taccgcgcag atacgctgga tatcaccttt
    37981 gacgtcacta aaaccacgct cgaagcgaca gtcaaagagc tgtgcgacaa agccgaaaaa
    38041 atggtacgta gcggcaccgt gctgctggtg ctctccgacc ggaatatcgc taaagatcgc
    38101 ctgccggttc cagccccgat ggcggttggc gcgatccaga cccgtctggt cgatcaaagc
    38161 ctgcgttgcg atgccaacat catcgtcgaa accgccagcg cccgcgatcc gcaccacttc
    38221 gccgtgttgc tgggcttcgg cgcgacggct atttatccat accttgccta tgaaacgctg
    38281 ggccgcctgg tagacaccca tgcgattgcc aaagattatc gtaccgtgat gctcaactac
    38341 cgtaacggca tcaacaaagg cttgtacaaa atcatgtcca aaatgggcat ctccaccatc
    38401 gcctcttacc gctgctcgaa actgtttgaa gcggtcggtc tacacgatga tgtagtgggc
    38461 ctgtgcttcc agggggcggt cagccgcatt ggtggggcaa gctttgaaga cttccagcag
    38521 gatctgctga atctgtcgaa acgtgcctgg ctggcgcgta agcccatcag ccagggcggc
    38581 ctgctgaaat acgtccacgg cggcgaatac cacgcctaca acccggacgt ggtgcgcacg
    38641 ctgcaacaag cggtacaaag cggcgagtac agcgactatc aggaatacgc gaagctggtt
    38701 aatgagcgtc cggcaaccac gctgcgcgat ctgctggcaa ttacgccggg tgaaaacgcg
    38761 gtcaacattg ctgatgttga accggcaagc gaactgttta aacgcttcga taccgctgcg
    38821 atgtctatcg gcgcgttaag cccggaagcc cacgaggcgc tggcggaagc gatgaacagc
    38881 atcggcggta actcaaactc cggtgaaggc ggtgaagatc cggcgcgcta cggcaccaac
    38941 aaagtgtcgc gcatcaagca ggtggcttcc ggtcgctttg gggttactcc ggcgtatctg
    39001 gtcaatgccg acgtcattca gattaaagtc gcccagggcg cgaagccggg cgaaggcggt
    39061 cagttgccgg gtgataaagt cactccttac atcgccaaac tgcgctattc ggtgcccgga
    39121 gtgacgctga tctccccgcc gccgcaccac gatatctact ctatcgagga cttagcgcag
    39181 ctcattttcg acctcaagca ggttaacccg aaagcgatga tctccgtgaa gctggtttcc
    39241 gaaccgggcg taggcaccat cgcgactggc gtggcaaaag cttatgccga tttaatcacc
    39301 atcgcaggct atgacggtgg caccggcgca agcccgctgt cgtcagtgaa atacgcaggt
    39361 tgcccgtggg agctggggct tgttgaaacc cagcaggcgc tggttgccaa tggtctgcgt
    39421 cataagatcc gtttgcaggt cgatggcggc ctgaaaaccg gtgttgatat catcaaagcg
    39481 gcgattctcg gcgcagaaag cttcggcttc ggcactggcc caatggtcgc gctcggctgt
    39541 aaatatctgc gtatttgcca cctgaacaac tgcgcaacgg gtgtggcaac tcaggatgac
    39601 aaactgcgta agaatcacta tcacggcctg ccgttcaagg tgacgaatta ctttgagttt
    39661 atcgcccgtg aaacccgcga gctgatggca cagctggggg taacgcggct ggtggatctg
    39721 attggtcgca ccgacctgct gaaagagctg gacggtttca ccgccaaaca gcagaaactg
    39781 gcgctgtcga agctgctgga gaccgccgaa ccgcatccag gtaaggcact ctactgcacc
    39841 gaaaacaacc cgccgtttga taacggcctg ctgaacgcgc agttgctgca acaggcgaaa
    39901 ccgtttgtcg atgaacgcca gagcaaaacc ttctggttcg atatccgcaa taccgatcgc
    39961 tccgtgggcg cgtcgctttc aggctatatc gcccagacgc acggcgatca ggggctggca
    40021 gccgatccta tcaaagcgta cttcaacggc accgcaggcc agagtttcgg tgtatggaac
    40081 gcgggcggcg tggaactgta cctgaccggg gatgccaacg actatgtcgg taaaggcatg
    40141 gcgggcggct taatcgccat tcgtcctccg gttggttccg ccttccgcag ccatgaagca
    40201 agtattatcg gcaacacctg cctgtatggc gcgaccggtg gtcgtctgta tgccgcaggc
    40261 cgcgcgggtg aacgtttcgg cgtgcggaac tccggtgcta tcaccgtggt agaaggcatt
    40321 ggcgacaacg gctgtgaata tatgacgggc ggtattgtct gtattctggg taaaaccggc
    40381 gttaacttcg gtgcgggcat gaccggcggc ttcgcttacg ttctcgatga aagcggtgat
    40441 ttccgcaaac gcgttaaccc ggaactggtc gaggtcttaa gcgttgacga tctggcgatc
    40501 catgaagagc atctgcgcgg tcttatcacc gagcatgttc agcatactgg ctctcagcgc
    40561 ggtgaagaga ttctggcgaa ctggtcaacc ttcgccacta aatttgcgct ggttaaaccg
    40621 aagtccagtg atgtaaaagc actgctgggt catcgtagtc gtagcgcagc agagttgcgc
    40681 gtgcaggcgc agtaaggggt agcaacaatg agtcagaatg tttatcaatt tatcgacctg
    40741 cagcgcgttg atccgccaaa gaaaccgctg aagatccgca aaattgagtt tgttgaaatt
    40801 tacgagccgt tttccgaagg ccaggccaaa gcgcaggctg accgctgcct gtcgtgcggc
    40861 aacccatact gcgagtggaa atgtccggta cacaactaca tcccgaactg gctgaagctc
    40921 gccaacgagg ggcgtatttt tgaagcggcg gaactgtcgc accagaccaa caccctgccg
    40981 gaagtttgcg gacgagtctg cccgcaagac cgtctgtgcg aaggttcctg cactctgaac
    41041 gatgagtttg gcgcggtgac catcggcaac attgagcgtt atatcaatga taaagcgttc
    41101 gaaatgggct ggcgtccgga tatgtccggc gtgaagcaaa ccggtaaaaa agtggcgatt
    41161 atcggcgcag gcccggcagg tctggcgtgt gcggatgtcc tgacgcgtaa cggcgtaaaa
    41221 gccgttgtct tcgaccgtca tccagaaatt ggcgggctgc tgaccttcgg tattccggcc
    41281 ttcaagctgg aaaaagaggt aatgacgcgt cgccgtgaaa tcttcaccgg catgggtatt
    41341 gaattcaaac tcaataccga agtgggccgc gacgtgcagc tggacgatct gctgagtgat
    41401 tacgatgccg tcttccttgg cgtcgggact tatcagtcaa tgcgcggcgg gctggaaaac
    41461 gaagacgccg atggcgtgta cgcagcgctg ccgttcctta tcgccaacac caaacagtta
    41521 atgggctttg gtgaaacccg cgacgaaccg ttcgtcagca tggaaggcaa acgcgtggtg
    41581 gtccttggcg gtggcgacac tgcgatggac tgcgtgcgta cgtccgtgcg ccagggagcg
    41641 aagcacgtta cctgtgccta tcgtcgtgat gaagagaaca tgccgggttc ccgccgcgaa
    41701 gtgaaaaacg cgcgggaaga aggcgtagag ttcaaattca acgtccagcc actggggatt
    41761 gaagtgaacg gtaacggcaa agtcagcggc gtaaaaatgg tacgtaccga aatgggcgaa
    41821 ccggatgcca aaggccgtcg ccgcgcggag atcgttgcag gttccgaaca tatcgttccg
    41881 gcagatgcag tgatcatggc gtttggtttc cgtccacaca acatggaatg gctggcaaaa
    41941 cacagcgtcg agctggattc acaaggccgc atcatcgccc cggaaggcag cgacaacgcc
    42001 ttccagacca gcaacccgaa aatctttgct ggcggcgata tcgtccgtgg ttccgatctg
    42061 gtggtaaccg ctattgccga aggtcgtaag gcggcagacg gtattatgaa ctggctggaa
    42121 gtttaagctc tgtcccagcg ccgcctcctc agaaaagagt cggcgctgaa attctccttt
    42181 ctttctggaa agcgcctcgc ataatctgaa gaaaaccaat gtaatacgct catctggatt
    42241 gttaccggta gatgcccggc gtatagctga ttccattatt caacgcagcg ccatataaag
    42301 ggacagataa tggaatcgct ctcagagaga acctcaacag gctaccagca aatccacgac
    42361 ggtattattc acctggtcga tagcgcccgg acggaaacgg tacgtagcgt taacgcgctg
    42421 atgaccgcga catactggga aattggccga cgaattgtcg aatttgaaca aggtggcgag
    42481 gcaagggctg cgtatggtgc gcagctaatc aagcgactat caaaggattt aagtctaagg
    42541 tataagcgtg gtttctctgc aaaaaactta cgccaaatga ggctttttta cctctttttt
    42601 caacatgttg aaattcgcca gacagtgtct ggcgaattaa caccattggg aattccgcag
    42661 acaccatctg cagaattccc atcggcaaag atttggcaga cgctgtctgc caaattgaga
    42721 ggctgtttga tatttccaca gcttttgtat tgcatctcct attttcggtt tgtatattca
    42781 tgcaaataaa cgaaggaaag ttgccgtgat ttacattgaa cagcttgaat taatccataa
    42841 aagtggtgat gtgttgtatc cagtaaaaat aaccagaaaa tcatcaggaa aaactgcctt
    42901 ccaccttgtt ccattcggct taaataagac tcacgacctt ctagaggtgg aagatgcttc
    42961 agaagcaatt cggctggtga ttgatgaacg tcattccatt cgctgcagta cacttacagc
    43021 aactattacg aataaaaagg gcaagcgaat taagcggaca ggtatataca gtatcaaggg
    43081 tgttaacata aaagaataca acgtcaggta atggcacaga gcggtccgtc agttcaggtt
    43141 tggctctgtg ccaaagatgg gtcaggtcac acctgatcta ataaatttaa gccattaaat
    43201 cagccttaac cttcaccacc acctttttaa tctcacccgg ctcgccaaca acgcatcccg
    43261 gcttatgcgg ctctcctagc ataaacacgg cgaacattcc cggtttcagg atgatggctt
    43321 gctcgttttc aatggtgctg caaagctggt aatcatcctc atggtggaac tcttcacact
    43381 gacgcgcagt gcctgccatg ccaaacagaa tccgttcttc accgtttaat aacagctgga
    43441 tatcaatgta ttgctcgtgc agctccgctt ttttctcaac gggcgattga gtgttaaacg
    43501 tcatgacatt cataaaaata ttgtcgccct gtaattcgta acgacccggc gctttttctt
    43561 gcggtctggc agctaatgcc agcgttaacg cgtcctgtaa cgcaggatgt aacccagcag
    43621 acggtaatga ccgtacttca cccatcatca taatttttct ccctgggcca acagcgctgc
    43681 cccaagtaaa cctgcatcat ggcggtagtg cgccgccagt aaatcaacat gaaataccgc
    43741 tggctcctgc gccagatacg tttccaccaa cgccagatac ccttctgcca gaccaacgct
    43801 gccaccgacc accacgcact ggcaatcagt tgtggcttta atatcagcga tcagccttgc
    43861 cagcacatgt gcggagcggt gaatcagttg ctgcgcctgc tcgtcgccct gcccggcgcg
    43921 tgtgaaaata gttctcgcat ccgcgcccat gagttcacct tgcgctgccg ctgcaatacc
    43981 gcgaccagaa gcaatcgctt ccacgcaacc tgtgcgtcca cagccgcaga ctgggccgtg
    44041 tggatcggca agcgtatgcc cgatatgccc cgccagaccg ccagggccgg taagcagttt
    44101 accgccgctc actacaccgc cgccgacgcc agtggaaacg gtgataaaca ccatctcggt
    44161 tacatcgcct tccagcgcct gatactctgc ccatgctgcg gcctgcgcgt cgttaatggc
    44221 aatggtcggc aaattggtaa gttgttccag cgttttgact aacggaaagt gtagcaatcc
    44281 accgagatta tgcggattca gcgccagcaa actgccgtcg cgaataattc cggttgaagc
    44341 gatggcaacc cgctgcgcat gagcttgcaa cggagagact aatgcggata aggcatcacg
    44401 caaggcttct ggtgtctggc tggctggcgt aggaagttca cgacgatcgc ggatctgccc
    44461 gtcagcgcca atcagcgcgg cggcaagttt agtaccgccg atatcaatcg ccagtgtggt
    44521 catagcaccg cctttttcat cgctgtgttg taccactgac aaatgtgttc aagacgcgta
    44581 atggcagaac cgaccgtcac cgcccacgcg ccgtggcgca tcgcatctgc cgcctgagca
    44641 ggcgtgttgt aacgcccttc ggcaatcacc cgacatccgg cttcgctcaa cgttttcacc
    44701 agcgccagat ccggctcttc tggcgtttca ggcgtggtat agccagaaag cgtagtgcca
    44761 ataatttcgg cgcccagctt ttggcatgcc aggccgtctt ccggcgttga gcagtcggtc
    44821 atcgccagta aaccgtgatg atgtatacgt gccagcagcg tttcaacagg caccggacgc
    44881 gggcggtcgg tgccgtcaat ggcgataatg tccgcgcccg cctgcgccag cgcatcgaca
    44941 tcttcaatat aggccgtgat gcgtaccgga gaatcctcca gatcgcgttt cacaattcca
    45001 ataatcggca cgctcaccac cgcacgcgtg gcttgcagat ttgccacacc ttcgatgcga
    45061 atggcaaccg cgcccgcctg ctctgccgct aatgccatgg ctgcgacgat ttcgggttta
    45121 tcgagcgggc tgtcaggaat cggctggcag gagacaatca ggccaccgtt agcagcgatt
    45181 ttttgatcca gttgtgcaag taacgacata catcttccct tagcgaaagg cccggtacat
    45241 agaccgggca acaggattaa cttttggttt tgactaaatc gtttttggcg ctgccaaatg
    45301 gcacggcacc gctaaatggt ttgccgtcga tagcgtcatg agtacgcaat gcttccgggc
    45361 gcagccaacg ctgaacgcgg gaaggcatat ccagaccaat cagcaggatc accacaaatg
    45421 tcaggctgaa cgagagcgat gccagcgcag tacccagatc cagacgttga gcgatcaacg
    45481 cgccgatgat tggggccagt gcgccaccca atgcgccaac gttgtaggta aagcccaggc
    45541 ccgctgcacg ctggtcggta tcgaaataac cgccaatcag tttcggtaag atcccggcga
    45601 tcccttgtcc aagcatttgc tggaagaaca gtaacagacc gagcacccag acgtttgcgc
    45661 cgccaatcgc aaataccgga ataatcagca gctgcgaggc cagcaggcta caaacgtacg
    45721 ctttgcgggt tcccagccag tcaccgagga agcctcctac gcagcatccc acggccgcgc
    45781 caaagccact aaagaacagc acattggcga cagtatgcgg gttataagcc agatcggttt
    45841 tcagatacgt tggcagcagc gcctgaatcg gccatgagta gaggaaagca aacaacacga
    45901 ccaccatcag cattacgccc gttggccagc gtttgcctgt actctgcacc ataaagctga
    45961 taaagattgc ggcgcataac agcccaagaa cagcgacgat cgcggcattt tgcaggttac
    46021 cggcgaagca gaaccacagc gcagtagccg ccgccagtgt cattacgata ttggcaatgc
    46081 gatgttcacc acggtagaga atatccacca ttgtgcgtac tggtgcttta cctgcgtgtt
    46141 tctctttcca gtcttccgct tccgggatgt ttttacgcag ccagagagca aagatgattg
    46201 gcaaaatgcc gataaagaac agcgcacgcc aaccccagac cggaaccacc aggctataga
    46261 cctgagcggc aacgacggcc cccacagaga agcctgaaat caaaaaacca ctggctttgt
    46321 tacgcagatg ttttggccag ctttcaatga cataggtggc gctggaaccg tattcacccg
    46381 ccatccccat gccgatgacc agacgagcga taaacatggt gatgtagcct ggcgcaaagc
    46441 cgcaggccag cgtcccggcc gagaagagaa cgatgctggt gaccattgcc agacgacgcc
    46501 cgtagcggtc acccatagcg ccgagcatca ggccgccgaa ccagcgagag ataaaggctg
    46561 cggagatcag acttgccgcc tgcaccgtcg tcagcccgaa ttcaccctgt acttcggtga
    46621 gtaccagggc gattaaaacg aaatcaaaac cgtcaagcag atatcccaac caggcagcgg
    46681 aaaatgcgcg ccattgtgca cggttgagat ggcgatacca cgggatattc tgggttgtag
    46741 tactcattgt gagtctcccg cggtgggcga tgcccacacg ctttggtatg aaaattgtag
    46801 ggtacagatg cgtttatttc ccctcacccg gtaggggcga gcgaggggaa aacaactcac
    46861 ccgcgctctt gcatcaactg ctgggccagc gccttcagtt ctggcagata tttttcatct
    46921 accggtccaa acggtttgcg gcacagcggc acagaaacga catccatata atgcaggaca
    46981 gttttcaggc cgcggaatac gccagttttg atcagtaaat caatgacttt gttgcactca
    47041 gtttgcagtt tctgcgcggt ctggatatcg ccttctttca gcgccttcac gatcccctga
    47101 tatcgccagc ccatgatgtt gtaagtactg ccgataccac catcagcgcc tgccagcaag
    47161 ccggaagcga agatttcgtc gtaaccgtta tagagcacca gatcaggatg ttcacgacgg
    47221 atctgttcca tctgatagag atcgccagaa gtctgtttca gcgcaccgac gccaggcaac
    47281 gtaaccaggg tgttgatctg atccagggtc agttttaccc cactcagggc tggaatgttg
    47341 tacacaacca tcggcaaacc atccgctgaa tcaataattg cccgatagtg atcgcagtgt
    47401 tcttcaaagc tgaaaggata gtagaacggc gtgacggcgg agacggcatc gaagccataa
    47461 cgtttagccg atgccgcaag ttgttggctt tcggcggtgc tgacgcaacc gacgtgggcg
    47521 atgagtttaa tcttaccttt cgcctcttcg gcgacgattt ccagtacctg ttcacgctcg
    47581 gaaaggcttt gtacaaaggc ctcgccggtc gaaccaccca cgtataaacc gtcgatgccc
    47641 tgctgaatat tgaactgaac caggcgacgc agactcgctt tatccagtgc ttgttgttgg
    47701 tcaaaaggag tcaggagtgc agccattacg ccacgtaaat tcgttgccat aaatacctct
    47761 gaagtgatac ttgtctgata aacgatatac ctttatacct gttataccag atcaattaag
    47821 caacacccca tacagaaagc ttataatgcg atctgcttca ctaaagtggc attatttctt
    47881 tttgttggtg gtctgaccga aagcgtgcca ggtagcagag acgctgttga gatgcgattg
    47941 caacgcacga tcggcttcgt caggatcatg acggcggatc gcatcaacga tcgcaatatg
    48001 ctgttgatag ctaacgttgt tatgctcgtg cagagcctga tcagccacgg ttggacgtgc
    48061 ggcgatcagc cagtcgagca gggcaacgtg gatcgccatg aagattgggt taccagggat
    48121 ctccgccagc acgcggtgga aatcaacgtc tgaacgaatg aatgccgcgt tgttatccag
    48181 cgactgactg ttgatttcca gtgcttttgc cagcaaatcg atttgctcat cggtggcatg
    48241 ttcagccgca tagcgcacca gactggattc aaagaacaga cgtaattgtt cgaaatgggc
    48301 aatcccaccg ggatgagaaa ggaaatcttt cgccatgccg gaaaactcac cgatgatagt
    48361 gtccgcagaa ggacgcgaga cgcgagcgcg ttcgccgttg tttatttgca ccagaccttt
    48421 gcgttttaac gctgccagcg cttcgcgcac agaaggacgc ccgacgttaa agaacgccat
    48481 cagttcgcgt tcagacggta attgttcacc ttcgccaaat tcacgacggc ggatcatctg
    48541 ttccagctct tcttccacca tttcggagag ttttttacgc gccagcgggc ggctacgcaa
    48601 gttgcgacca attgcaggtg aagaatcttc ggtttgcgaa tcaaatgcgt tcataaggcc
    48661 cattctgtaa ggtcagtgtg attaacatca tcagtgacat cctatcacag gattgaaagt
    48721 aggggaaaat ggcaggtttt tctctttgtg cctcatcatt accataatta acggaataat
    48781 taactattgc gaaaaattaa tgtaacgcag ataaacacat cccgattgaa ttattca
//
LOCUS       DAZPDU010000008        47726 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZPDU010000008 DAZPDU010000000
VERSION     DAZPDU010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47726
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.008"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            86..718
                     /gene="comR"
                     /locus_tag="ACHURS_000411"
     CDS             86..718
                     /gene="comR"
                     /locus_tag="ACHURS_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFR1236232.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(780..1319)
                     /locus_tag="ACHURS_000412"
     CDS             complement(780..1319)
                     /locus_tag="ACHURS_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFR1236233.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1546..2850)
                     /gene="ndh"
                     /locus_tag="ACHURS_000413"
     CDS             complement(1546..2850)
                     /gene="ndh"
                     /locus_tag="ACHURS_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFR1236234.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3258..3800)
                     /gene="ycfP"
                     /locus_tag="ACHURS_000414"
     CDS             complement(3258..3800)
                     /gene="ycfP"
                     /locus_tag="ACHURS_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFR1236235.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3823..4848)
                     /gene="nagZ"
                     /locus_tag="ACHURS_000415"
     CDS             complement(3823..4848)
                     /gene="nagZ"
                     /locus_tag="ACHURS_000415"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFR1236236.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELCFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(4859..5683)
                     /gene="thiK"
                     /locus_tag="ACHURS_000416"
     CDS             complement(4859..5683)
                     /gene="thiK"
                     /locus_tag="ACHURS_000416"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFR1236237.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQIGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5664..6305)
                     /gene="lpoB"
                     /locus_tag="ACHURS_000417"
     CDS             complement(5664..6305)
                     /gene="lpoB"
                     /locus_tag="ACHURS_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFR1236238.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6319..6696)
                     /locus_tag="ACHURS_000418"
     CDS             complement(6319..6696)
                     /locus_tag="ACHURS_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFR1236239.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVSIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6699..7058)
                     /gene="hinT"
                     /locus_tag="ACHURS_000419"
     CDS             complement(6699..7058)
                     /gene="hinT"
                     /locus_tag="ACHURS_000419"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFR1236240.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7392..9581
                     /gene="fhuE"
                     /locus_tag="ACHURS_000420"
     CDS             7392..9581
                     /gene="fhuE"
                     /locus_tag="ACHURS_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFR1236241.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQLDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLVFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNMEGSIVYGAPRNFSITGTYQF"
     gene            complement(9641..11074)
                     /gene="ptsG"
                     /locus_tag="ACHURS_000421"
     CDS             complement(9641..11074)
                     /gene="ptsG"
                     /locus_tag="ACHURS_000421"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFR1236242.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11369..12166)
                     /locus_tag="ACHURS_000422"
     CDS             complement(11369..12166)
                     /locus_tag="ACHURS_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR1236243.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(12177..13181)
                     /gene="holB"
                     /locus_tag="ACHURS_000423"
     CDS             complement(12177..13181)
                     /gene="holB"
                     /locus_tag="ACHURS_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFR1236244.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13178..13819)
                     /gene="tmk"
                     /locus_tag="ACHURS_000424"
     CDS             complement(13178..13819)
                     /gene="tmk"
                     /locus_tag="ACHURS_000424"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR1236245.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(13809..14831)
                     /gene="yceG"
                     /locus_tag="ACHURS_000425"
     CDS             complement(13809..14831)
                     /gene="yceG"
                     /locus_tag="ACHURS_000425"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFR1236246.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14834..15643)
                     /gene="pabC"
                     /locus_tag="ACHURS_000426"
     CDS             complement(14834..15643)
                     /gene="pabC"
                     /locus_tag="ACHURS_000426"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFR1236247.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(15763..17004)
                     /gene="fabF"
                     /locus_tag="ACHURS_000427"
     CDS             complement(15763..17004)
                     /gene="fabF"
                     /locus_tag="ACHURS_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFR1236248.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLDITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(17092..17328)
                     /gene="acpP"
                     /locus_tag="ACHURS_000428"
     CDS             complement(17092..17328)
                     /gene="acpP"
                     /locus_tag="ACHURS_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFR1236249.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17539..18273)
                     /gene="fabG"
                     /locus_tag="ACHURS_000429"
     CDS             complement(17539..18273)
                     /gene="fabG"
                     /locus_tag="ACHURS_000429"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFR1236250.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18286..19215)
                     /gene="fabD"
                     /locus_tag="ACHURS_000430"
     CDS             complement(18286..19215)
                     /gene="fabD"
                     /locus_tag="ACHURS_000430"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFR1236251.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKLMQEAVPEGTGAMAAIIGLDDASIAKACEEAAAGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19231..20184)
                     /gene="fabH"
                     /locus_tag="ACHURS_000431"
     CDS             complement(19231..20184)
                     /gene="fabH"
                     /locus_tag="ACHURS_000431"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFR1236252.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20252..21322)
                     /gene="plsX"
                     /locus_tag="ACHURS_000432"
     CDS             complement(20252..21322)
                     /gene="plsX"
                     /locus_tag="ACHURS_000432"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFR1236253.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21403..21576)
                     /gene="rpmF"
                     /locus_tag="ACHURS_000433"
     CDS             complement(21403..21576)
                     /gene="rpmF"
                     /locus_tag="ACHURS_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFR1236254.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21628..22149)
                     /gene="yceD"
                     /locus_tag="ACHURS_000434"
     CDS             complement(21628..22149)
                     /gene="yceD"
                     /locus_tag="ACHURS_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFR1236255.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            22348..22932
                     /gene="yceF"
                     /locus_tag="ACHURS_000435"
     CDS             22348..22932
                     /gene="yceF"
                     /locus_tag="ACHURS_000435"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFR1236256.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENACLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(23044..24003)
                     /gene="rluC"
                     /locus_tag="ACHURS_000436"
     CDS             complement(23044..24003)
                     /gene="rluC"
                     /locus_tag="ACHURS_000436"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFR1236257.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLIAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            24576..27761
                     /gene="rne"
                     /locus_tag="ACHURS_000437"
     CDS             24576..27761
                     /gene="rne"
                     /locus_tag="ACHURS_000437"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFR1236258.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTGVPVAAAVEPVVSAPVVEEVAEVVEAPVQIAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEVTVEHNHATVPMTRAPAPEYVPDAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(27956..28909)
                     /gene="flgL"
                     /locus_tag="ACHURS_000438"
     CDS             complement(27956..28909)
                     /gene="flgL"
                     /locus_tag="ACHURS_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFR1236259.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(28921..30564)
                     /gene="flgK"
                     /locus_tag="ACHURS_000439"
     CDS             complement(28921..30564)
                     /gene="flgK"
                     /locus_tag="ACHURS_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFR1236260.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITVANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQELDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFTIGKPA
                     VLQNTKNKGDVAIGATVTDASVVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEKDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(30630..31571)
                     /gene="flgJ"
                     /locus_tag="ACHURS_000440"
     CDS             complement(30630..31571)
                     /gene="flgJ"
                     /locus_tag="ACHURS_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFR1236261.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(31571..32668)
                     /gene="flgI"
                     /locus_tag="ACHURS_000441"
     CDS             complement(31571..32668)
                     /gene="flgI"
                     /locus_tag="ACHURS_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFR1236262.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTESLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(32680..33378)
                     /gene="flgH"
                     /locus_tag="ACHURS_000442"
     CDS             complement(32680..33378)
                     /gene="flgH"
                     /locus_tag="ACHURS_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFR1236263.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(33431..34203)
                     /gene="flgG"
                     /locus_tag="ACHURS_000443"
                     /pseudo
     CDS             complement(33431..34203)
                     /gene="flgG"
                     /locus_tag="ACHURS_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
     gene            complement(34375..35130)
                     /gene="flgF"
                     /locus_tag="ACHURS_000444"
     CDS             complement(34375..35130)
                     /gene="flgF"
                     /locus_tag="ACHURS_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFR1236264.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHQVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(35150..36355)
                     /gene="flgE"
                     /locus_tag="ACHURS_000445"
     CDS             complement(35150..36355)
                     /gene="flgE"
                     /locus_tag="ACHURS_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFR1236265.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(36380..37075)
                     /gene="flgD"
                     /locus_tag="ACHURS_000446"
     CDS             complement(36380..37075)
                     /gene="flgD"
                     /locus_tag="ACHURS_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFR1236266.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(37087..37491)
                     /gene="flgC"
                     /locus_tag="ACHURS_000447"
     CDS             complement(37087..37491)
                     /gene="flgC"
                     /locus_tag="ACHURS_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFR1236267.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNMVKSMMLKTLTLGQ"
     gene            complement(37495..37911)
                     /gene="flgB"
                     /locus_tag="ACHURS_000448"
     CDS             complement(37495..37911)
                     /gene="flgB"
                     /locus_tag="ACHURS_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFR1236268.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            38066..38725
                     /gene="flgA"
                     /locus_tag="ACHURS_000449"
     CDS             38066..38725
                     /gene="flgA"
                     /locus_tag="ACHURS_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFR1236269.1"
                     /translation="MLAIKRSVAIISILFTPLSAASNLTSQLQTFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRQDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGNGFSANAEGQALNNAAVTQNARVRMVSGLVVSGVVDADGNILIN
                     Q"
     gene            38801..39094
                     /gene="flgM"
                     /locus_tag="ACHURS_000450"
     CDS             38801..39094
                     /gene="flgM"
                     /locus_tag="ACHURS_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFR1236270.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDASVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            39099..39515
                     /gene="flgN"
                     /locus_tag="ACHURS_000451"
     CDS             39099..39515
                     /gene="flgN"
                     /locus_tag="ACHURS_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFR1236271.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(39555..41090)
                     /gene="murJ"
                     /locus_tag="ACHURS_000452"
     CDS             complement(39555..41090)
                     /gene="murJ"
                     /locus_tag="ACHURS_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFR1236272.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSLAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(41200..42123)
                     /locus_tag="ACHURS_000453"
     CDS             complement(41200..42123)
                     /locus_tag="ACHURS_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR1236273.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSADPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVLQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(42125..42772)
                     /locus_tag="ACHURS_000454"
     CDS             complement(42125..42772)
                     /locus_tag="ACHURS_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFR1236274.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(42783..43367)
                     /gene="rimJ"
                     /locus_tag="ACHURS_000455"
     CDS             complement(42783..43367)
                     /gene="rimJ"
                     /locus_tag="ACHURS_000455"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFR1236275.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            43603..44811
                     /gene="mdtH"
                     /locus_tag="ACHURS_000456"
     CDS             43603..44811
                     /gene="mdtH"
                     /locus_tag="ACHURS_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFR1236276.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRCRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSALLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     ATRRLLERDA"
     gene            44875..45522
                     /gene="grxB"
                     /locus_tag="ACHURS_000457"
     CDS             44875..45522
                     /gene="grxB"
                     /locus_tag="ACHURS_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFR1236277.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            45656..46216
                     /locus_tag="ACHURS_000458"
     CDS             45656..46216
                     /locus_tag="ACHURS_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR1236278.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLISQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            46322..47368
                     /gene="pyrC"
                     /locus_tag="ACHURS_000459"
     CDS             46322..47368
                     /gene="pyrC"
                     /locus_tag="ACHURS_000459"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFR1236279.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLITCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            47442..47687
                     /gene="dinI"
                     /locus_tag="ACHURS_000460"
     CDS             47442..47687
                     /gene="dinI"
                     /locus_tag="ACHURS_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFR1236280.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
BASE COUNT        11549 a        12904 c        11868 g        11405 t
ORIGIN      
        1 tatttttatg ggatctggac tggtgaacgg aggtgattat ttttataata acctttcatt
       61 cacagtcacc aggttcaacg gcatcatggc aactgactca acacaatgtg taaaaaaaag
      121 ccgtggccgc ccaaaagtgt tcgacaggga tgccgcgctt gataaggcca tgaaattgtt
      181 ctggcaacac ggttatgaag cgacttctct tgcggacctc gtcgaagcga ccggagccaa
      241 agcgcccacg ctatacgcgg aatttaccaa caaagagggg ttatttcgtg ccgttctcga
      301 ccgctatatc gatcgttttg ccgctaagca tgaagcacag ctgttttgtg aagagaaaag
      361 cgtggagtct gcgctggctg actattttgc tgccatcgcc aactgcttta ccagcaaaga
      421 caccccggca ggctgcttca tgatcaacaa ctgcaccacc ctctccccag attcaggaga
      481 tatcgccaat acgttgaaat cacgccatgc gatgcaagag cgcactttgc agcagttttt
      541 atgtcaacga caagcgcgcg gggaaatccc gacccactgt gacgtgacac atctggcaga
      601 attccttaat tgtattattc aggggatgtc gatcagcgca cgcgaaggtg catcgctgga
      661 aaaactgatg cagattgccg gaacgacttt gcgtttatgg cccgaactgg tgaaataagt
      721 cgcaaaaaaa gcccctcagc ctgagcgaat gaggggccaa attgcagagt acagccttgt
      781 tatactttgt tattcaaaat cagttgcccg ttactatcta gcgggatctg cgtacccgga
      841 tcgcggtcca tgcggatttt gccctgctga tcgccaatct tataggtcac atcataaccg
      901 agcatttttt ctgacttgtc atacaccgtt ttacaacgct gttgcgtagt cgtgtaagta
      961 tcgctttcct ggagagagcc ctggatctgg ttaccggcat atccaccacc cagcgccccc
     1021 acaacagtgg cgacatcttt accacgacca ccaccaaact gatgccctat cacgccgcca
     1081 gcaacagcgc cgagcaccga cccggtaatg cgattttcat cctgcaccgg tcgacgatgg
     1141 gtcactgtga cgttgcgaca ctcctgacgc ggtgttttaa ccgtttcctt gattggggtt
     1201 gcagaaacaa cctgagcgta ttgcgggccc cgttcaaaca cgttcagact ggccactgcc
     1261 gctacgccca gcgcagctgc gacaccaatc ccgatacccg ccaacattga tttattcacg
     1321 ggaacacctc cttctttgca gttatgccaa tcatgcaagc gaaggagaaa agcagctatc
     1381 agacagaaga tgaaaagcgc gggaatggct ggaaatactt aaggattcgg agaagtctga
     1441 aggaatgcct gatgcgacgc ttaacgcgtc ttatcaggcc taccgctcgt accatttgtc
     1501 cagggcggat gaggcgttta tgccacatcc gccagtgtac gtcgattaat gcaacttcaa
     1561 acgcggacgg ataacgcggt taatactccc caccagcatc attaatccgg ttttaaagta
     1621 accatgcagc gcaatctgat gcattcggta tagcgagata tatacaaagc gcgcaattcg
     1681 tccttcaatc atcattgagc cgcgcgtcag gttacccatc aggctaccga cagtggagaa
     1741 gttcgacagc gataccagcg aaccatgatc tttatactga taatttttca gcggtttacc
     1801 gttcatctgc gccagaatat tgttcattgc gcaggtcgcc atctggtgtg cagcctgagc
     1861 acgcggcgga acaaagcccc cttccggacg cgggcatgac gcgcagtcgc caatagcgta
     1921 aatgtctgga tcgcgggtgg tttgcagcgt cggttccacc accagctggt tgatacggtt
     1981 ggtttcaaga ccaccgatat ctttcaggaa gtctggcgct ttgatcccgg ctgcccacac
     2041 catcagatca gcctcaatat attcaccatc tttagtgtgc aggccgcctt catcagcact
     2101 ggtgaccatg gtttgcgtca gcacgcgaac gccaagtttc gttagctcgt tgtgggccgc
     2161 agcagagata cgtggcggta atgcaggcaa aatacgttct cccgcttcta ccagcgttac
     2221 gttcagggct tcgttggtca ggcctttgta accgtagctg tgcagttgct tgaccgcatt
     2281 gtgcaattca gcggagagtt ctacacccgt cgcgccgccg ccgacaatcg caatgttcac
     2341 tttaccattc gcgcccaggt tggcggagta tttcaggaac agattcagca tctcctggtg
     2401 gaagcgacgc gcctggtgcg ggttatcgag gaaaatgcag ttctctttga cacctggcgt
     2461 attgaaatcg ttagaggtgc tacccagcgc cattaccagg gtgtcatagg cgattttacg
     2521 ttccggaacc agcagttcac ctttctcatc gcgcagttct gcaatagtga ttgttttcgc
     2581 ttcacgatca atatcaatga cggaacccag ctggaactgg aaaccatgat tgcgcgcatg
     2641 ggccagatag ctcaacgcat cgacgccttc atcaagcgag ccagtcgcca cttcgtgcag
     2701 cagcggtttc cacagatggc tgtggttacg atcgaccagc gtaattttgg cttttttctt
     2761 gcgtcccagc ttatgcccca gctgtgttgc catttccagc ccaccagcac cgccgccgac
     2821 aatcacaatt tttttcaatg gcgtagtcaa cgtgaccccc ttaaatttat taaccaattg
     2881 ttaattaatg gttataaaca tagctcttaa ttaacaacag gttacagcaa tgaaaatgtt
     2941 cttcgctatt gagaataaca tgaatggtgc attggtcata ccaaaattga tgttaatcaa
     3001 gttttgttgc tgaaaagata ggcgacttta ccgggtgaga tcaaaaaaca gagtttattg
     3061 tttattattt gagcaagtcc ggaagggtga tatagggtga cgcgtatatc gagggcgttt
     3121 ggtcgtcctg ataagatgcg cagcatcgca tcaggcattg atgctcgact gtcggatgcg
     3181 gcgtaaacgc cttatccgac ttacagaatt cggagcgcat caggtaccgg caaggttacc
     3241 tgatgcgacg agggcattta cccgagggtt ttgaacgcct taatgcgctg caaatgcggg
     3301 gagatattct tgaatttgtg cgtctgctct tcgtcccaga caatctcgta ataatgatgc
     3361 aactcttcag atgtccgctg gctgttaagc gcttcatcat tacgcgacaa aatcaccagg
     3421 caacgatcgc gattcttctc gcggaagttg gtcacacact tagtggcaat atcggcatac
     3481 tcttccgggc gatcaatctt gccttccatg ttctcataag ggaacaaatt agggttgaag
     3541 atcacctggc ggatatcgca aagaaaacca atccgttccg cccagtatcc gcccagacca
     3601 acgccgcaaa tcagcggacg ctcgtcgacg ttcagttgca acattttgtc cacttctttg
     3661 agcagatgct gcatatcatg tttcggatgc cgcgtactgt agcttatcaa gcgtacatcc
     3721 gggtcaataa actgcaattg taagactttc tcgtggttac ccggactgtt agagtcaaaa
     3781 ccgtgtaaat agataatcat cgcttcctca cataagccag ggttagtgac ctgctttctc
     3841 ttcctgccag cgttcatgta actgattcag acgggtgctg atcgccttcc agcgagccga
     3901 gtccatcagt tcctgtcgcg aaaatgaacc tttatgatac aaacgtgtaa cacgttctgc
     3961 cttgatcggt gacagattat ctaacacgct gacggcccct ttacgattat tgcagaccag
     4021 gatcatatcg caacctgcgt ccagtgatgc ctgcccgcgt tcggcataac tgcccataat
     4081 cgcggcacct tccatcgata aatcgtcaga gaaaattaca ccgtcaaaac acagttcctg
     4141 acgcaaaacg gttttcagcc agtaggaaga accgctcgcc ggacgcggat caacatcact
     4201 gtagatcaca tgcgcaggca taatggcgtc gagtttattt tcgcggatta aggaactgaa
     4261 gaccgacata tctttagcgc gaatctccgc ttgtggacgt ggatcgcacg gtgtttcttt
     4321 gtgtgagtct gccgttactg caccgtgtcc tgggaagtgt ttcccggtcg ttttcattcc
     4381 ggcttcatgc ataccatcaa taaaccggct ggcaattgcc agggcttttt gtggatcggc
     4441 atgataagaa cgctcgccaa tcgccgcgct gatatgcccg acatccagca ctggcgcaaa
     4501 gctgatatca atatccatag cgatcatttc gctggccatc agccaacccg cctcttgcgc
     4561 cagtttgccg ccctcttcca ttcccaacag cgcagcaaag gattgtgctg ccggtaagcg
     4621 ggtaaaacct tcgcgaaaac gctgcacgcg tccaccttcc tgatctaccg ccaccaccag
     4681 atgattgcgc gatgctgcgc ggatctggcg caccagttca cgtaactggg caggatcatg
     4741 atagttacgc gtaaagagaa tcagccctcc caccagcgga tgcgccagta tttcacgctc
     4801 ttccgcgtcc agttcgtaac cttcgacatc caacattact ggacccacac tgctctcctt
     4861 attgttttat taatagctgc cgccaggtgt catcggccag cctgataaat tgttgatcgc
     4921 cgatttgtcg ccagcggtac tcaaaccacc ctgctttgag catcagcagc cagggaaacc
     4981 atcgcctgac ctgacgccat aattgcgccg gataaatctt cgcgcgagtg gcatagtcat
     5041 tgaccaattg ccggtgctgt tcagtatttt ccacccacac cgccgccagt tccagcgcga
     5101 tatcaccatc tccggcatac tcccagtcga tgagttttaa ccctgacgcg ctatgcacta
     5161 aatttccggc gtggacatcc atatgcaatg gacttaagcg taaaggccgt ggttcccgcg
     5221 ctttgcgcag acgttttaac attcgcagcc aacccacagt ccgccgcgcc ggatcgcttt
     5281 gctgccagta cagttccagt aagggcaaca gcgttattcg ccagccaaaa cgtggttgtt
     5341 gatgaagata atacagcaag cctgccagtt cgttggtatc cggcaaatac gtttttaccg
     5401 cgccgggcag atagtcgact accatccagt cacggagata taaatgcggc ttcggtgcaa
     5461 tgcttgcggg tagttgtgat aaagcccgat actggcgcaa gaacgcggac tgcggcgcat
     5521 caggatcgtg cggctgacgc acaacaaaac gctggccctg atgttcaatg agaaaactac
     5581 cgccactaag cccactatta aacgtcgtga cgggatgaaa ctgcgggaaa aagcgcgaca
     5641 gcaattcgtc gcgcgtgatg ggattattgc tgcgaaacgg cacctttacc tgaccagata
     5701 atttcgcccg tctgcaccag catcagctgc atttgtaggg tcggagcgtt aacgttgcca
     5761 gaggcgctgg agtacagcac gtaatgagcg ccgacattgc gggcaatgcc tatggcttta
     5821 ctacgggtac ctaaactgtc ctgcggcgac aaacctaact gttgcttagc catcgacagc
     5881 tgctgggcgg aaaccagggt aaatttcccg ttattagcca gcgcatttcg cagcgtttcg
     5941 gtcgcttctg cggcattcag cgaaccgtta gtacggttgt taacgctatc aaccagcagg
     6001 acgctacccg cagtcacccc gtcagcccca agcatcttac tgaccatcgg ctgcattgcg
     6061 ccattccagt cataatggcg aatatgcggc gcaggcggtg ccgtttgatc ttcgtgctca
     6121 attgggcctg gctgctgcgg gatcgtcggc accgagggca ctgtggggac aggctgttgt
     6181 ggctcggctg gttgttccgg cgctggtttc acttcttcta ccggtgcagg ttcacgttgc
     6241 cccacacacc cggcgagaaa catcgccagc gcggtaatca aggcgtagcg actcattttt
     6301 gtcatcaaga ttcacccctt acaaatatag ataaagtctg actttgtgcg cccccagaaa
     6361 attggcgctg ccgtacagcg ttaccgccga atgtgcggga atgctaacgc tgcgtggcct
     6421 ttccagagga tgcatctcca gccctctggc gtcataccag taaaaacgat aatgaacggt
     6481 aacgggttct tgcctttcgt tataaagcgt tgaggatgct gaaggttgaa tatcagacgt
     6541 cgaaaggacg ggcttttctg cactgatgcc cgcagccaat aaagatgact ccatcaccag
     6601 cgattgctca tcattcaccg gaatttccgg atgtgaacga cagcccacca gcagtaacaa
     6661 cgccagagac accagcccaa agcatccttt tctcatcgtt acagaccttt atgcgccagc
     6721 attggtccca gcggacggcc acccaacaag tgcatatgga tgtggtaaac ctcttgtccg
     6781 ccatggcggt tggtattcat gatcagacga tagccatctt cggcaatacc ttcttgctca
     6841 gcaatttttg ccgctacggt gatcatgcgt cccagcgcct gctcatgctc agctgacacg
     6901 tcgttcacag tcggaatgag gatattcgga atgatcagaa tatgcgttgg cgcttgcggc
     6961 gaaatatcgc gaaacgccgt taccagatca tcctggtaga cgatatcgga ggggatctca
     7021 cgacgaataa ttttgctgaa tatagtttct tctgccacga cattttcctt tttcaaatag
     7081 ccatgcgttg cgccatgcta cgccgcgcca cactgcgagt atagagtatg gtcgagttaa
     7141 acctgctctt tcaaccttaa ctcacgattt cttaagcaaa aaatcgtttt gctgccgtat
     7201 atatcgccat tattcccatt tctgcctgcg aatgcgctga aaagacattc tctgtttggt
     7261 ttacacgaca aatatgaatg cgcatatttc tcatttgcat ttacaaacaa aattattcgc
     7321 acactaaata taaccgctca tcaccctcct ccggcatgag cctgacgaca acataaacca
     7381 agagatttca aatgctttca acacaattta acagggataa tcaatatcaa gccatcacca
     7441 aaccgtcact acttgccggt tgcatagcac tggcactatt accttctgcc gcttttgctg
     7501 caccagccac tgaagaaacg gtgattgttg agggttcagc cacagctcca gatgatggcg
     7561 aaaatgatta cagcgtaacg tctacctctg cgggtaccaa aatgcagatg actcaacttg
     7621 atattcctca gtcggtcact attgttagcc agcagcggat ggaagatcag cagttacaaa
     7681 cgctgggcga agtgatggaa aacacgctgg ggatcagcaa aagtcaggcg gattccgatc
     7741 gtgctcttta ttattcccgt ggattccaga tcgataacta tatggttgat ggtatcccca
     7801 cctattttga atcgcgctgg aatctgggcg acgcactttc tgatatggca ctctttgaac
     7861 gcgtagaagt agtgcgtggc gcgacaggac tcatgaccgg gacgggtaat ccatctgcgg
     7921 caattaatat ggttcgaaaa cacgcgacca gtcgtgaatt taaaggcgat gtctcggcgg
     7981 aatacggtag ctggaacaaa gaacggtatg tggcggattt acaaagccca ctcaccgaag
     8041 acggtaaaat ccgcgcgcga attgtcggcg gctaccagaa taacgactca tggctggacc
     8101 gctacaacag tgaaaagacc ttcttctcgg gcattgtcga tgctgattta ggcgatctta
     8161 cgacgctttc agccggttac gaatatcagc gcattgatgt taatagccca acctggggcg
     8221 gtttaccgcg ctggaatact gatggcagca gcaacagtta cgatcgtgca cgcagtaccg
     8281 cacctgactg ggcgtacaac gataaagaga tcaacaaggt ctttatgacc ctgaagcagc
     8341 ggtttgctga tacctggcaa gcgacactga atgccaccca ctctgaagtc gaatttgaca
     8401 gcaaaatgat gtatgtcgat gcctatgtaa acaaagcgga tggtatgctg gttgggccat
     8461 acagtaatta tggacctggc tttgattatg tcggcggcac cggttggaac agtggcaaac
     8521 gtaaagttga tgcgctggat ttgttcgctg acggtagtta tgaattgttt ggtcgtcagc
     8581 acaatctagt gtttggtggc agttacagca aacaaaacaa tcgttacttc agttcatggg
     8641 ccaacatctt cccggatgaa attggcagtt tctacaactt taatggcaat ttcccacaaa
     8701 ccgactggtc accacagagc ctggcacagg acgataccac acatatgaaa tcgttatatg
     8761 ctgctactcg agtcaccctt gccgatccgc tgcatctgat cctcggcgca cgttatacca
     8821 actggcgggt tgatacgctg acttacagca tggagaaaaa ccacaccacg ccttacgctg
     8881 gtctggtgtt tgacatcaat gacaactggt cgacctacgc cagctatacc tctattttcc
     8941 agccgcaaaa tgatcgtgac agttcaggca aatatctggc tccaatcacc ggtaacaact
     9001 acgagctggg tctgaaatcg gactggatga atagccgtct gaccaccacg ttagccatct
     9061 tccgtattga gcaggataat gtcgctcagt ccaccggtac acctatcccc ggcagcaacg
     9121 gcgaaaccgc ctataaagcg gtggatggga cagtcagtaa aggggtggaa tttgaactca
     9181 acggcgcaat taccgacaac tggcagctga catttggcgc aacgcgctat attgcagagg
     9241 ataacgaagg aaacgccgtt aatcctaatc tgccacgcac cacggttaaa atgttcacca
     9301 gctatcggtt gcctgtcatg ccagagttga cagtcggcgg tggtgttaac tggcaaaatc
     9361 gcgtgtatac cgacaccgtg acaccgtatg gcaccttccg cgccgagcaa ggtagctacg
     9421 cgctggtgga tctcttcacc cgctaccagg tgacgaaaaa cttctcgtta caggggaacg
     9481 tcaataacct gttcgacaaa acctacgata ccaacatgga aggttctatc gtctacggcg
     9541 caccgcgtaa tttcagcatt accggcacgt atcaattctg aataacacct gtaaaaaagg
     9601 cagccatctg gcggccttag tctccccaac gtcttacgga ttagtggtta cggatgtact
     9661 catccatctc ggttttcagg ttatcggatt tagtaccgaa aatcgcctga acaccagaac
     9721 cagcaaccac tacgcccgct gcgcccagtt tcttcaggcc agcctgatcc actttagaca
     9781 catcagcaac gctgacgcgc agacgggtaa tacatgcgtc gaggttagta atgttttctt
     9841 taccaccaaa tgcagcaacc agagccggtg ccatttcgct ggtacctgtt gcttttgcat
     9901 cttcagtcgc gtcttcacga cccggcgttt tcagatccag tgctttaatc agcacgcgga
     9961 agatggtgta gtaaacaatc gcataaccga taccgacgat cgggaacagc cacagtttgc
    10021 tgctgttacc agacagaacg ataaagtcga tcagaccgtg cgagaacgac gtaccgtcac
    10081 gcatccccag aagaatacag attgggaatg ccaggcctgc cagaatcgcg tggatgatgt
    10141 acaggatcgg cgcaacgaac atgaaggaga actcgatcgg ctcggtgata ccggtcagga
    10201 acgaggtcag cgccgcggag atcataatac cgcccacttt cgcgcggttt tctggtttag
    10261 cagagtgcca gatagcaatt gcggcagctg gcagaccgta cattttgaac aggaagccac
    10321 cagacagttt acccgcagtc gggtcacccg ccatataacg cggaatgtcg ccgtggaaaa
    10381 cctgacctgc tgcgttggtg tattcaccaa tctgcatctg gaaaggtacg ttccagatgt
    10441 ggtgcagacc aaacggcacc aggcaacgtt cgatgaaacc gtaaatgcca aacgcaacta
    10501 ccgggttctg gtaagcagcc cactgagaga aggtctggat tgcagaacca atcggcggcc
    10561 aaatgaagga cagcacaacg ccagtaaaga tggcagccag gccagaaatg atcggcacaa
    10621 agcgtttacc ggcaaagaag ccaagatact caggcagctt aatacggtag aaacggttaa
    10681 acatgtacgc tgcgatcgca ccggagataa tccctccgag tacgccagta tccgccaggt
    10741 gtttagaggc gatttcttca gcaggtaaat gcagtaccag tggcgcaacc acggccatgg
    10801 ttttaaccat gatgccatag gcaacaactg cggccagcgc ggatacgcca tcgttattgg
    10861 taaagccgag ggcgacaccg atcgcaaaaa tcagtggcat gtttgcaaag acggaaccgc
    10921 ctgcttctgc cataacatgc gatacaacgg cgggcagcca gctgaaattc gcggaaccga
    10981 cgcccagcag aatacctgcg ataggcagta cggataccgg cagcatcagc gatttaccga
    11041 ccttttgcag gttagcaaat gcattcttaa acataattga gagtgctcct gagtatgggt
    11101 gcttttttta cgttctcacg cgtggcaagg ggggagagcc tcgccgtgta cagggcatct
    11161 aagcgccctt tatttattac acagagtaaa ataattcagt gccgatttgt ttgacggcta
    11221 tcacgtttca acttcagatc attggagaag ttgcacattt tgacaaaaca tatgtcacaa
    11281 tatttcaaac gtggttattc accgcccaat tttcggcggt gaactttact cgttttagcc
    11341 attaattacg agctttaaaa aaactcattc aacggatgga ttgaaggcgg gaagcgtcga
    11401 tgtgaaacag acgggcgaag ttatcggtgg ttacctgcgc cagttcttca acggcaacac
    11461 ctttcaacac agccatgtat tctgcaacgt cacgaaccat cgcaggttga ttctcttttc
    11521 ctcgatgcgg taccggcgca aggtaaggtg agtcagtttc caccagtaac cgatccaggg
    11581 ggacataacg cgcagcatct cgaagttgct ccgcattacg gaaggtcaca atgccggaaa
    11641 aggagatgta aaatccgaga tccagtaatt tacccgccgt ttctctgtcc tctgtaaaac
    11701 agtgtagtac gccaccgcaa tccgtcactt tttcttcgcg cagaattgcc agcgtatcgg
    11761 cgcgggcgtc acgggtatgg acgataaccg gcttgttcag ttcacgacca atctggatat
    11821 gatggatgaa ggactcttgc tgacgaactt tagtttccgg tgtgtaataa taatccagcc
    11881 cggtttcacc cagcgctaca acaccctctt ctgccgccag acggcgtaaa tcttctacat
    11941 cgtagggatc attctggttg agtggatgca cgccacaaga aaatacgacg ttgtcacgtt
    12001 cgcctaccag atcccgcata tgtaaataac ccggtaatgt tgtggcgact gccagacaaa
    12061 atttcacatc gcgtgcggcg gctttcgcca gaacgtcatc cacgtcctta tgcaaagatt
    12121 cataatccag accatcgaga tggcagtgtg agtcgactaa aaacatgatg tctctcttaa
    12181 agatgaggaa ccggtagcac aacgcccggt tgcaggtaat gctcaatacg cagtaaaaga
    12241 tcggtgatga gaagctcgcg gttgatgcct gtgacagaca ttaactgttc acgaatgtgg
    12301 caaacatccc ccagtatagc ctgcaggcgc gagggagaaa gatggtttgc cagttcgacg
    12361 acaaggcccg gcacatcaac attggtcacc tgcgcagcac catgatggcg ttttagcgca
    12421 tccatcagca acgttgccag ccagtgtaaa cgcgccggag cttgttcatg attaagggcc
    12481 gccagcagcg aataccagtc gcccgatggc acgctatatg ccaatgcctg acacaatgtt
    12541 tcacgagcct gccagttatc tccctgaaac aacgccagtg ccgcgccagg cgaaccggcg
    12601 cttaagcgca atgcggcaag taatgcatcc tgtgacattg tcacttcgcg tgaaagccag
    12661 gtcacggcgt actgttccgg cggcggcgca aggtaatgta accgacaacg actacgtaat
    12721 gttgccagta aacgttcagg ctcgcgggta gccaggaaaa accaggtttc tgctggtggc
    12781 tcttcaagcg ttttcagcaa tgcgttagcc gcggcgtcgg tcagtaaggc agcatcggtt
    12841 acccagacaa ctttcgcacc acctaggcgt gcgtgctcat tcagcttttc ggtgacctca
    12901 cgtactgcat caatgcccag cgtatttttc cctttttcgg gagccagggt gtagtaatcg
    12961 ggatgcgtgc cagcctgcat caactgacat ccacgacagt gaccgcaact tttgtggccc
    13021 tgcggttgtt ggcagagtaa ataacggctc agggcgtaga ttaaagcatc atcgcccatg
    13081 cccggtaacg cctgaatgag tagcgcatgg tgacctcttc cggcctgata gctggctacc
    13141 agtttttcga aatcaggtcg taaccatgga taccatctca tgcgtccaac tccttcaccc
    13201 agtgggtcac ggtagtgcgg attgcatcca tcacggcctc cagcggctgg gtggcatcaa
    13261 tggtatgaat acttttatct tgtgcagcca gttccagata gcgggcgcgg gtgcgattaa
    13321 agaaatcgaa agactcttgc tcaatacgat ccagctcgcc gcgcgcacgc gcgcgtttta
    13381 agccaacttc cggggtaaca tcgagataga gcgttaagtc ggggcgaaaa tccccgagaa
    13441 cagcatcacg cagtgttgcc agcatatgtt ggtcaatacc acgtcctccg ccctgatacg
    13501 cctgagtgga gagatcgtgg cgatcgccaa tcacccaggt gccgttagcc agagctggtt
    13561 tgatgaccgt ttctaccagt tgaacgcgcg cggcataaaa catcagaact tcggctttat
    13621 cggtaatgac ttcatcgcct accgatttga tatccagcac caggcttctt aacttttcgg
    13681 caagttgcgt accgccaggt tcccgagtga aaaccatgtc gcggataccc agttgctcaa
    13741 gcgtctcaac caccacatta cgcgcggtag ttttgcctgc gccttccagc ccctcaatga
    13801 cgatatactt actgcgcatt tttttcctta agcactttca gataatcctg cacagacttg
    13861 ttatggctgg caagattggt attaaacgtg tgaccacctt taccatcggc cacaaaatag
    13921 agatacggcg tttttgccgg atgcgcagca gccttcagcg aatccgcccc cggcgtagct
    13981 atcgcacccg gcggcaaacc ggtaatggta taggtgttat acgctgtcgg cgtttccagg
    14041 tctgcacgag aaagtttgcc attataacgc tctcccatcc cgtaaatcac ggttgggtcg
    14101 gtctgcaggc gcataccaat gcgtaaacgg ttgataaata ccgaggcaac ctgatcgcgt
    14161 tcactggcaa cggcggtttc tttttcgata attgatgcca tcgtcaccag ctgattttta
    14221 tctttataag gcagaccgtc cgcacgccct tcccaggcgc tatcgaccgc tttcaccatt
    14281 ttcttgtgcg ctcgcttgag taacgcgaca tcggtggtat tggcggtata catccaggtg
    14341 tctggccaga accaaccttc aatccactcc gggttttcca gttcaagtgc ctgcgctacg
    14401 gtggcgtact tatcgtcact cagcgtgtgc ttgatatacg gagcctcacg caattgcttg
    14461 aggtagtcgc tcagacgcat cccttctacc agtcgcagcg ggaactgtgc ttctttaccg
    14521 ctttccagca atttcagcat ctcgcgcacg gtcatctgcg gtgtaaagcg gtaagtcccg
    14581 gctttaaagt gagaaagatc cggttcgata cgcagcaacc attgaaaaac ccgtgggcga
    14641 ttgatgatct tatcggcata aagctgttca ccaagcgcca gacgtccggt ccctggcttc
    14701 agggtaaata tcgtctcttc tttgataagc aatttgctgt cggcaagatg gcgaaccttc
    14761 cagacgccca caccagcggc gatacccagt accaccagca ataacaagat aatcaataac
    14821 acttttttca tgactaattc gggcgctcac aaagtggggc taaatattca tataacgttg
    14881 ctgacgaaaa ggagacatcg ccacaggcac gtacgggcat cactggcatt aacgcattac
    14941 aaataaccat ctcatctgcc tgcaacgcct cttccagaga ggcttgcact tcgacaagct
    15001 gataagagga ttgtgccagc aaacggatac agaattgtcg cataatgccg ttaacacctg
    15061 cctgatccag acgcggcgta taaactacat tgcccttccg ccagaacaaa ttagccgcac
    15121 agcattccgt aacccaccct tcgctgtcaa ggaccagcgc ctcatcagcg tttgtctgct
    15181 caagatgaga gcggatcaat acttgctcaa gccgattaag atgtttaata cctgcaagat
    15241 gaggattgcg ccccagccgc accgggctta gcgccaacgt catcccttcg ttacgcaaac
    15301 ggtcgtaatg tgcaggataa accgtaacgg agagaatccg cgttgccggt ccactgttca
    15361 atgtgctgta ccctcgcccg ccactaccgc gactgatcac gactttcagc acaccatttt
    15421 gctgttctgc tgccagcgtt ttcatctctt gttcaagctg aggccagaag tcacagaaaa
    15481 tcatcaaccg ctgacaagca tcctgtagtc gctggatatg cgccgataac aaactgactt
    15541 taccgtcgat aactctggcg gtggtaaaac aaccatcacc aaactgcgtt gcccgatcgc
    15601 ttactgccag cgattcctgc ttatgaccgt taattaagaa cataccggct ccttatcaga
    15661 gaattgttag tgtggcagca tgttcactac ggaacaagtc ggaataaaag ctaagaaaaa
    15721 aggcccgcaa gcggaccttt tatatgggtg ggaaatgaca acttagatct ttttaaagat
    15781 caaagaacca ttagtgccac cgaagccgaa ggagttacac agcgtgtatt ccattccgct
    15841 gacctgacgt gcttcatgcg gtacgaaatc cagatcgcaa ccttcatccg ggttatccag
    15901 gttgatggtt ggcggaacag cctgatcgcg cagcgccagg atggagtaga tagattctac
    15961 tgcacccgcc gcacctaaca ggtgaccggt catagatttc gtggagctta ccaacacacg
    16021 gcttgcagct tcaccgaaga tggttttcac cgcctgcgct tcagctttat cgccagccgg
    16081 cgtagaagta ccgtgcgcgt taacgtagcc aatctgactc gcttcaatgc ctgcatcacg
    16141 cagagcattt gccatcgcca gagctgcgcc tgcgccattt tctggcggtg acgtcatatg
    16201 ataagcatcg ctgctcatac caaagccgac gagttcagcg taaattttcg caccgcgttt
    16261 tttcgcgtgt tcgtactctt caagtaccag cataccggca ccatcgccca gtacgaagcc
    16321 atcacgctct ttatcccacg ggcggctcgc cgcttgcggg ttatcattgc gggtagataa
    16381 tgcacgtgcc gcgccaaaac caccaacacc cagcggcgta ctggctttct ctgcgccacc
    16441 tgcaaccatc acgtcagcat cgccatacgc gataatacgc gcagcatggc caatgttgtg
    16501 cacgccggaa gtacaggcgg tcgcgataga gatgcttggg ccacgcaggc catacatgat
    16561 agtcagatga cctgccacca tgttcacaat cgttgacgga acgaagaatg ggctgatctt
    16621 acgtggacca ccgttcatca gagatgtgtg gttttcttcg atcagtccga ggccgccaat
    16681 cccggagcca attgcggcac caatgcgggt tgcgttctct tccgttatat caaggccaga
    16741 atcctgcatg gcctgaacgc cagcgacaat tccatattga atgaaggcat ccatcttgcg
    16801 ctgttctttg cgcgagataa tgtcctcaca gttaaaatcc tttactaagc cagcaaattt
    16861 cgttgcatag gcgctagtat cgaaatggtc gattaggctg atgccactct gaccggcaag
    16921 cagagctttc caggtagact ctacggtatt gccgacagga gacaacatgc ccagtccggt
    16981 cacaactaca cgacgcttag acacgtttgt cctccaggga gggaaaaaat gattctagtg
    17041 ggacaaaaag ataaaactca ggcggtcgaa cgaccgcctg gagatgttca cttacgcctg
    17101 gtggccgttg atgtaatcaa tggcagcctg aacggtggtg attttctcag cttcttcgtc
    17161 cggaatctca gtatcaaact cttcttccag agccattacc agctcaacgg tgtcaagaga
    17221 atccgcgccc aggtcttcaa cgaaagaagc attgttggta acttcttcct gcttaacgcc
    17281 cagctgttcg ccgataattt tcttaacgcg ttcttcgata gtgctcatac tcttaaattt
    17341 cctatcaaaa ctcgctttcg cgatggtttt cgtagtgtat aaaatgttga aaaatttgca
    17401 actaaatccc ggcaggtctt accacgattt tacgttattt tgaggcctta ccccccaata
    17461 acgcaaatca ttttgcacta attattactc ataaccacgc agactgcgcg caacatgagc
    17521 attttgtgca aatcgcggtc agaccatgta catcccgccg ttcacatgca aagtttcacc
    17581 cgtgatgtaa gctgcttcgt cggatgccag gaatgcaacc gcgttggcga tttcctgtgc
    17641 gccgccgagg cgacccgcag gaacctgcgc caggataccc gcacgctggt catcgctcag
    17701 cgcacgtgtc atgtccgttt caataaagcc cggagcaaca acgtttacag taataccgcg
    17761 tgacgcaact tcgcgcgcca gtgatttact gaagccgatc aagcccgctt tcgccgcagc
    17821 gtagttggcc tgaccgccat ttcccatggt accaaccaca gaaccgatag tgataatacg
    17881 accatgacgc tttttcatca tagcgcgcat taccgctttt gacagacgga aaacagatga
    17941 aaggttggtt tcgataatat cgttccactc ttcatctttc attcgcatta acaggttatc
    18001 acgagtgata ccggcattat tgaccaggat atccacttca ccaaattctg cgcgaatttt
    18061 ttccagaaca gattcgatag atgccgggtc ggtcacattc aacatcagac ctttgccgtt
    18121 ggcacctaaa taatcgctga tcgcctgagc gccattttca ctggtcgcag tgccaataac
    18181 tttcgcgcca cgggctgcga gcgtttcagc aattgcgcgg ccaattccgc ggcttgcacc
    18241 ggttaccagt gcgatttttc cttcaaaatt catgattttc ctcttttaaa gctcaagcgc
    18301 cgctgccatc gctgaaggtt cgttcagcgc cgaggcggtc agggtgtcga caatgcgttt
    18361 cgtcaggcca gtaagcacct tgcccgggcc gacttcatag agatgttcta cgccttgcgc
    18421 tgccatgtac tcaacagact tcgtccactg aaccgggtta tacaactgac gtaccagtgc
    18481 gtcacggatg gcatcaccat tggtttcgca tttcacatca acgttattca caacagaaac
    18541 tgttggtgcg ttaaaggtga ttttcgctaa ttctactgcc agtttgtcgg ctgctggttt
    18601 catcagcgca cagtgagacg gtacgctcac tggtaacggc agagcacgtt ttgcgcccgc
    18661 agctttacag gcagcgccag cacgctcaac cgcttcttta tgaccggcaa taaccacctg
    18721 tcccggagag ttaaagttta ccggagaaac gacctgacct gctgcagctt cttcacacgc
    18781 tttcgcaata gacgcatcat ccagaccgat gattgccgcc atagcgcccg taccttccgg
    18841 tacggcttct tgcatgagct tgccgcgcat ctcaaccaga cgcaccgcat cagcgaaatc
    18901 aatcacacca gcgcaaacca gcgcggaata ttcccccagg ctgtgaccgg ccatcattgc
    18961 cggtgcttta ccgccctgct gctgccatac gcggtacagc gcaacagagg cagtcaacag
    19021 cgctggctgg gtttgccagg ttttattcag ttcttcagct ggcccctgct gggtcagcgc
    19081 ccacaggtcg tagcccagcg ccgcagaagc ttcagcaaac gtttcttcga caattggata
    19141 gctcgccgcc atatcagcca gcattccaac ggtttgagaa ccctgtccag ggaacacaaa
    19201 tgcaaattgc gtcatgtttt aatccttatc ctagaaacga accagcgcgg agccccaggt
    19261 gaatccaccg ccaaaggctt caagcagaac caactgcccc ggcttaatgc gcccgtcgcg
    19321 tacagcttca tccagcgcgc acgggacaga ggccgcagag gtattaccgt ggcgatccag
    19381 cgtcaccacg acattgtcca tagacatacc gagttttttc gccgttgcac tgataatacg
    19441 caggttagcc tgatgcggaa ccagccagtc cagttgagaa cggtcaagat tatttgccgc
    19501 cagcgtctca tcaacgatgt gcgccagttc cgttaccgca accttgaaga cttcgttgcc
    19561 cgccatcgtc agatgaattg aattctctgg attcacacgg tcagcattag gcagcgtcag
    19621 caactcacca tagctaccgt cggcatgcag atgggtggag atgattcccg gctcttcaga
    19681 ggcagccagc accgcagcgc ccgcgccatc gccaaaaata ataatagtcc cacgatcggt
    19741 tggatcgcag gtgcgcgcca gtacatcgga accgacgacc agagcatact tcaccgcccc
    19801 agatttcacg tattgatcgg ctacgcttaa tgcataggtg aaacctgcgc aggctgctgc
    19861 aacgtcaaat gccgggcaac ctttaatgcc cagcatgctt tgaatctgac aagctgcgct
    19921 cgggaaagcg tgcgtagcag aagtcgttgc cacaacgatc aggccaatct ggtctttctc
    19981 aatgcccgcc atctcaattg cgcgtgtcgc cgcttcaaag cccatggttg aaacggtttc
    20041 gtttggcgcg gcaatgtggc gttcgcggat accggtacga gtgacaatcc actcgtcaga
    20101 ggtgtccacc attttttcca aatcggcgtt tgtccgcact tgttcgggca gatagctgcc
    20161 agtaccaata atcttcgtat acatgtacgc tcagtcactt ttcggttata taccgtcact
    20221 tgcaaactgc gagttcgctg gcagcgtcct gctaccgcag agttccgctt ttgccaccgt
    20281 ccagcagctc aaaaccagct gggtatacag attccaggcg agcggcaatt cgctgaggaa
    20341 cttgtcgctg caccgcctgc actgcctgtt caatcgcgac cgcaaaagct cgctgattgg
    20401 ctgcaccatg actttttatc accgtgccgc gcaatcctaa cagacaggcg ccgttatact
    20461 ggtcggggtt gaggtgactg aatcgcctcg tcaggctctt ttgtagccaa cgctttaata
    20521 acagtagcca ccacgaccgt tttttccctt caccctgaga tttcagcaga gaaaggaaca
    20581 tcctgacaac accttccatc gtctttaatg tgacatttcc tgtaaagccg tcacaaacca
    20641 gcacatctgt cttgccagtt aacaactcat tggcttcaag atagccgata taattgatag
    20701 aagggattgt tttaagcacc gctgaggcat cccgaatact gtcgagaccc tttacttctt
    20761 cttcaccaat attgagcaac gccacgcgag gattgggaat ttccaccacc tcttcagcca
    20821 gaactgagcc cataatggca aattgcacca acattgtact gtcacaatcg acgttggccc
    20881 ctaagtcaag gaccaccgtt ttgccctttt gctgatgtgg taataccgtc accagcgccg
    20941 gacgctcaat cccctccagg ggcttgagta ataattttgc cagccccatc agtgccccgg
    21001 tattaccggc actgacacag gcttgcgctc gaccttcttt caccagctcc agggccacgc
    21061 gcattgaact cccacgactg gcgcggatag cttgcgaagg ccgggcatca ctggcgataa
    21121 ctgactgcgc aggaataatc tgcagacgcg aacgttgttc aaagtcagct ttagcaagta
    21181 atggcgtgat ggcgtcggga ttgccgacta aaagaagagt gagttgcgaa ttagaattca
    21241 gtgcctgcaa tgctgcaggc actgtcacgg aagggccaaa atcccctccc atgacatcta
    21301 acgccagggt tagacgtgtc aaggtatcgt cgccgcccgg tttggtcttt ccccagttgc
    21361 ctggggaaaa tcctcactaa gcttcatcac gcagacgcgt gattacttag cgatgacctt
    21421 gcggccgcgg tagtaaccgt cggcagtgat gtggtgacgc aggtgttttt caccagaagt
    21481 tttgtctaca gacaggctgg tgactgcggt cagcgcgtca tgggaacgac gcatgccacg
    21541 tttggaacgg gttggtttat tctgttgtac ggccatggac cttactcctc aattacggtt
    21601 tatccccgat ccaacgggga gcaccaatta cttacgcttt aagctggcta atacggcaaa
    21661 tgggtttggc ttttgcactt cttcaggcag ttcaccaaag accatgtccg cttcggacac
    21721 ttcacagtgt tcagaatcat gcaccggaac taccggcaag gcgaggatga tttcatcttc
    21781 aaccattgca agcagatcga tttcaccgaa ttcgttaacc tcaatcggtt catacgcttc
    21841 cggcagtgct tcagcctgtt cgtctgaacg cacaggacta aaacaatacg ttgtgtagac
    21901 ctgatgagta aacggcttcc cgcaacgctg acactcgagc gttaccgtca ccttcgcatc
    21961 gccgtttaac actgcgagac gttggttatc gatagcgaac gacatggagc attccacatc
    22021 actgtccaca ctgactacgg attcggcgac gcgctcaacc tgatcagggg tatagatacc
    22081 ctggtaatca aggcgttttt gagccgtacg aaccggatcg agagtcaggg gtaattttac
    22141 cttttgcata gggcgcgcat attaactttg taacgtcata gagtcaaaga aaaaggcagc
    22201 ctgtggttgc cttttgccaa taattcgcac acattgcggg ttactgcttt attttcgttc
    22261 agtgctgata acgaacatca acctgcaaaa cgaagcgcat agtttaaaat ggctatcatc
    22321 aatacgctat atctgatgga aaaaaatatg cctaaactta ttttagcctc cacctcgcca
    22381 tggcgtcgcg ctcttctgga aaaactgcaa atctcttttg aatgtgcagc accagaggtc
    22441 gacgaaaccc cacgcagcga cgaatcaccg cgacagttgg tgcttcgact ggcacaagaa
    22501 aaagcgcaat ctctggcgtc acgttatccg gatcatttaa ttattggttc agaccaggta
    22561 tgtgttcttg atggcgaaat caccggtaaa ccgttaacgg aagaaaatgc ctgtctgcaa
    22621 ttacgcaaag ccagcggcaa tatcgtcacc ttctatactg gactggcgtt gtttaattcg
    22681 gcgaatgggc atctacaaac agaagtggag ccttttgacg tccatttccg tcacctgagc
    22741 gaggcggaga ttgataatta cgtgcgtaag gagcatcccc tgcactgcgc gggtagcttt
    22801 aagagtgaag gatttggcat tacgctgttt gagcgtttag agggacgtga ccctaacacg
    22861 ctggttggtt taccgctaat cgcgctgtgt cagatgttac gtcgggaagg gaaaaacccg
    22921 ctgatgggat agttgtttgc gctggatgcg gttacgtcgc atccggcaat cggtgcaggc
    22981 ggcctgatgc ggcgtaaacg ccttatcagg ccaaccgccc attatcaggc ttatatgctt
    23041 gtattagcgc gcgttacgca gcttttgcaa acaacgcttc aaaccttcat ccatcggcgc
    23101 ttcgatacgc atcacctcac cggtccccgg atgggtaaac ttcaacgctg cggcgtgcag
    23161 gaacagacga tttaatcccg tgcctgcttc agtgagctgt ctgtcaaatt cacggtcacc
    23221 gtagcgatcg tcaaaggcaa tcggatgacc cgcatactgt gtatgcacac ggatctgatg
    23281 agtacgtccg gttaccgggc tacaacgcac cagggtggca aatgcatagc gttcttccac
    23341 tttaaagcgt gtttccgacg gtttgccttc ctgactgaca cgcacgatac gttcgccgct
    23401 ttgcagaata tttttcaata acggcgcttg aacgctcttc acatgcgact gccactgacc
    23461 gcgcaccagc gccaggtaat ctttttgcat tcctttttca cgtaattgct catgcagaga
    23521 acgcaacgcc gagcgttttt tcgctatcag caacacacct gaagtgtccc ggtcgagacg
    23581 atgaaccagt tcaaggaacc gcgcttccgg gcgcaacgcc cgcaaacctt cgataacgcc
    23641 gaagcttaaa ccactgccgc catgtaccgc cgtaccggaa ggtttattca gcaccaggat
    23701 atgatcatct tcatataaga tgacgtccgc cagcgccgcc accttttgca gatgtggcga
    23761 aaccgcctct tcttcccgct cagcaacgcg aaccggtggg atacgcacct catcacccgc
    23821 ttcgagttta tattcaggct taatacgttt tttgttcacc cgcacttcgc ctttacgcaa
    23881 aatacggtaa atcatacttt ttggtacgcc tttcaattgg gtacgcaaaa agttatcgat
    23941 acgttgcccc gcttcgtcag cggtgatagc aacaattttt acggatggag tctctgtttt
    24001 catggtgggc gattctaatt agccaacagg attcgcgcca ctcatttttc tatgcttata
    24061 tttagttttg caccttatta cttcactacg tgatcacttt attgatggtt attaaaccaa
    24121 tcaccagcaa gaagtgaaaa aactgtgagt aagcgggtga taaatggtaa aagtcatctt
    24181 gctataacaa ggcttgcagt ggaataatga ggccgtttcc gtgtccatcc ttgttaaaac
    24241 aagaaatttt acggaataac ccattttgcc cgaccgatca tccacgcagc aatggcgtaa
    24301 gacgtattga tctttcaggc agttagcggg ctgcgggttg cagtccttac cggtagatgg
    24361 aaatatttct ggagagtaat acccagtctg tttctttgat aattgcgctg tttttccgca
    24421 tgaaaaacgg gcaaccgaca ctctgcgcct ctttgagctg acgataaccg tgaggttggc
    24481 gacgcgacta gacacgaggc catcggttca cacccggaaa ggcgttactt tgcccgcagc
    24541 ttagtcgtca atgtaagaat aatgagtaag ttacgatgaa aagaatgtta atcaacgcaa
    24601 ctcagcagga agagttgcgc gttgcccttg tagatgggca gcgtctttat gacctggata
    24661 tcgaaagtcc agggcacgag cagaaaaagg caaacatcta caaaggtaaa atcacccgca
    24721 ttgaaccgag tctggaagct gcttttgttg attacggcgc tgaacgtcac ggtttcctcc
    24781 cactaaaaga aattgcccgc gaatatttcc ctgctaacta cagtgctcat ggtcgtccca
    24841 acattaaaga tgtgttgcgt gaaggtcagg aagtcattgt tcagatcgat aaagaagagc
    24901 gcggcaacaa aggcgcggca ttaaccacct ttatcagtct ggcgggtagc tatctggttc
    24961 tgatgccaaa taacccgcgc gcaggtggca tttctcgccg tatcgaaggc gatgaccgta
    25021 ccgaattaaa agaagcactg gcaagccttg aactgccgga aggcatgggg cttatcgtgc
    25081 gcaccgctgg cgtaggcaaa tctgctgagg cgctgcaatg ggatttaagc ttccgcctga
    25141 aacactggga agccatcaaa aaagccgctg aaagccgccc ggctccgttc ctgattcatc
    25201 aggagagcaa cgtaatcgtt cgcgcattcc gcgattactt acgtcaggac atcggcgaaa
    25261 tccttatcga taacccgaaa gtgctcgaac tggcacgtca gcatatcgct gcattaggtc
    25321 gcccggattt cagcagcaaa atcaaactgt ataccggcga gatcccgctg ttcagccact
    25381 accagattga gtcgcagatc gagtccgcct tccagcgtga agtccgtctg ccgtccggtg
    25441 gttcgattgt tatcgacagc accgaagcgt taacggccat cgacatcaac tccgcacgcg
    25501 cgacccgcgg cggcgatatc gaagaaaccg cgtttaacac caacctcgaa gctgccgatg
    25561 agattgctcg tcagctgcgc ctgcgtgacc tcggcggcct gattgttatc gacttcatcg
    25621 acatgacgcc tgtacgccac cagcgtgcgg tagaaaaccg tctgcgtgaa gcggtgcgtc
    25681 aggaccgtgc gcgtattcaa atcagccata tttctcgctt tggcctgctg gaaatgtccc
    25741 gccagcgcct gagcccatca ctgggtgaat ccagccatca cgtctgcccg cgctgctccg
    25801 gtaccggtac cgtgcgtgac aacgaatcgc tgtcgctctc tattctgcgt ttgatcgaag
    25861 aagaagcgct gaaagagaac acccaggaag ttcacgccat tgttcctgtg ccaatcgctt
    25921 cttatctgct gaatgaaaaa cgttctgcgg tgaatgcgat tgaaacgcgt caggacggtg
    25981 ttcgctgcgt gattgtgcca aacgatcaga tggaaacccc gcactaccac gtgttgcgcg
    26041 tgcgcaaagg ggaagaaacg ccgaccttaa gctacatgct gccgaagctg catgaagaag
    26101 cgatggcgct gccgtctgaa gaagagttcg ctgaacgtaa gcgtcctgaa caacctgcac
    26161 tggcaacctt tgccatgccg gatgtgccgc cagcgccaac cccagctgaa cctgccgcga
    26221 ctgtcgtagc cccagcaccg aaagctgcaa cggcaacacc agcagctcct gcacaacctg
    26281 ggctgttaag ccgcttcttc ggcgcactga aagcgctgtt cagcggtggt gaagaagcca
    26341 aaccgaccga gcaaccaaca ccgaaagcag aagcgaaacc ggaacgtcaa caggatcgtc
    26401 gcaaacctcg tcagagcaac cgccgtgacc gtaatgagcg ccgcgacacc cgtagtgaac
    26461 gtactgaagg cagcgataat cgcgaagaaa accgtcgtaa tcgtcgccag gcacagcagc
    26521 agactgccga gacgcgtgag agccgtcagc aggctgaggt aacggaaaaa gcacgtacca
    26581 ccgacgagca gcaagcgccg cgtcgtgaac gtagccgccg ccgtaacgat gataaacgtc
    26641 aggcgcaaca agaagcgaag gcgctgaatg ttgaagagca atctgttcag gaaaccgaac
    26701 aggaagaacg tgtacgtccg gttcagccgc gtcgtaaaca gcgtcagctc aatcagaaag
    26761 tgcgttacga gcaaagcgta gccgaagaag cggtagtcgc accggtggtt gaagaaactg
    26821 ccgctgccga accaattgtt caggaagctc cagctccacg cacagaactg gtgaaagtcc
    26881 cgctgccagt cgtagcgcaa actgcaccag aacagcaaga agagaacaat gctgataacc
    26941 gtgacaacgg tggcatgccg cgtcgttctc gccgctcgcc tcgtcacctg cgcgtgagtg
    27001 gtcagcgtcg tcgtcgctat cgtgacgagc gttatccaac ccagtcgcca atgccgttga
    27061 ccgtagcgtg cgcgtctccg gaactggcct ctggcaaagt ctggatccgc tatccaattg
    27121 tacgtccgca agatgtacaa gttgaagagc agcgcgaaca ggaagaagta caagtgcagc
    27181 cgatggtgac tggggtccct gtcgccgccg ctgtcgaacc tgttgttagc gcaccggttg
    27241 ttgaagaagt ggctgaagtt gtagaagccc ccgttcagat tgccgaaccg caaccggaag
    27301 tggttgaaac gacgcatcct gaagtgattg ccgccgcagt aactgaacag ccgcaggtga
    27361 ttaccgagtc tgatgttgcc gtagcccagg aagttgcaga acacgcagaa ccggtggttg
    27421 aaccgcagga agagacggca gatattgaag aagttgccga aactgctgag gttgtggttg
    27481 ctgaacctga agttgttgct caacctgccg cgccagtcgt cgctgaagtc gcagcagaag
    27541 ttgaaacggt agctgcggtt gaacctgagg tcaccgttga gcataaccac gctacggtgc
    27601 cgatgacgcg cgctccggca ccggaatatg ttccggatgc accgcgtcac agtgactggc
    27661 agcgccctac ttttgccttc gaaggtaaag gtgccgcagg tggtcatacg gcaacacatc
    27721 atgcctctgc cgctcctgcg cgtccgcaac ctgttgagta ataattagct caagtaatca
    27781 agccctggta actgccaggg cttttttatt tcatctttga atcaacatat taaaattcat
    27841 cgaatggcat ccttgctaac caacaatgca aaataggcaa aaaaatgccc gccccggcga
    27901 tcccagagca ggcagacaaa acatacccag tttcatgata tgttttaaag cgaaattatt
    27961 tgttgagctg gaagagcgac aatccctgca tatcggtaaa tgctttatac gatgcctgca
    28021 atgccgtttg ctgcatgatg taagatgaaa tagttgcatt ccagtccaca tcaaccagat
    28081 cgctcatctg ctgcgtttgc cctaaagcgc gatcgctacc taatgaatcc agcgactcca
    28141 gttcgttcag ctgcgtgcct aattccgcgc gaacggtcag cacattgttc agcgagtttt
    28201 tcagtccgcg gttggtttta tctaacgccg cagcggcggt ttctttatcc gcttcgctat
    28261 ccgcgaccgg cgttttcagg gctgcgatgg cgctatccag catggcaaaa agattggttt
    28321 cagaagcgct accgtctggt tccgctaccg cgttactggt aatactgtcg aaaattttgt
    28381 cgcccgtatg tcctatcacc atcgaacgcg aagcatcgac ctgttgctta atactttctg
    28441 ttccgccgac ataatcaccg ttagcctcac taaacggcgc agtctctgtt ttataaccgg
    28501 caaaaatgta gcgcccatta ccgtcagtgg tgttcgccag attcagcaac tggtcacgaa
    28561 gcccctgaat atccgtagcc agcgaggccc ggtcatcgtc actcaaggtg ccattgctgg
    28621 cgtagacaat tttttcctga gcattctgga tagcagtggt gacctggcta agtacactct
    28681 cttccagtga cactttttga gtggcgaaag tacgcgccag cgtgtactgg ctgttttgcg
    28741 cctgtgcctg ggagagaaca accgcttgtg atgcagcaat gggatcatct gaagggttaa
    28801 cgactcgctt acccgtcgac atctgttcgc cgtacttcat ccattctgcc tgagaattgg
    28861 tgataccacg catgttttgc tggtacatca tctgggtact gaaacgcatt ttatctcccc
    28921 ttagcgaatg ttaatcagcg catcaaaaat cgcgtttgcc gtctgcagaa cctgcgcatt
    28981 cgccaggtaa tactgctgaa aacgttgcag atttccgtac tcctcatcga gattgacacc
    29041 ggaaatcgac tgctgctgat tggaaagctg cgtcaccaca ttaccttgcg tggtgctact
    29101 ggttttcaac gtcgcggttt tattaccgat atcactcact aacgaggcat aagcgtcgtt
    29161 aaaggatttc gcaccgccca ccgttttact gttgctttgc agatccagca gggcctgacc
    29221 gttgcggtta tcgctatcac ccgcatcttt ttcgctcgcc atcgctattt tcgcttcgtc
    29281 ggtgattaat acatccatgt tgacgatggc gtcacttact ggtttcagcg tgaagctgtc
    29341 gttaacggca ggcgttcctg taaacgtcaa ctccagacca tcaaatgcca ctttaccgtt
    29401 ggcatccggc gtcaccgtaa aagtggtatt gctggcaagg cgggtgacct gccactgatt
    29461 attatcgaac gagattttgt aatctgtcgc cagtaccacg gaggcatcag ttaccgtggc
    29521 accgatcgca acgtcacctt tgtttttcgt gttttgcaga accgcgggct taccgatagt
    29581 aaagaaatct tcaccagcat cgccgttagc atcaaatccg gctttgtgtt gggtgttgaa
    29641 agcctcggca aatgccagcg ccagttgtcc aagcgtatta cgcgtctggt ccagctcctg
    29701 agaacggaat gtcagaatgc cgcccagcga cccggtattc agtaatttct ccgggatctc
    29761 aatattgcct gccgtcccat caacataagc gacagtcgta cgagaagggt cagcgctgga
    29821 aggaactgcc gccagttgcc gcgccgtact tccctgaacc agtgagtaac cattggccac
    29881 cgtgatgtta taagtgccgc catcctgaac gctgacttct acaccaacaa tctggtttaa
    29941 ttcgctcacc agttgatcgc gttgatccag cagattgtta ggtgacgccc ctgcccccac
    30001 gcctgtcagg cgcgagattt gatcgttcag gctggcaatt tgtttagcgt aattgttgat
    30061 ctgatcaacg ctggcaccta tcgcgatatt gacctgtttg tcctggtcgc gcagatattg
    30121 atcggtggtt ttaaactgat tcaccaaccc ttctgatttc ccaatcagcg cctggcgcgc
    30181 tgccgggtct tccgcgttac tcaccagcgt ttgcaggctg gtgaagaaat cctgcatctg
    30241 tgttgccagc gaagaggtac tggtggagag catattgtcg attttcgaca tctgctcata
    30301 gcgggcggtc aggccgctac tttgcgtctg cgcagcacgt aactggttgg taataaacgc
    30361 atcatactca cgctgcacac cagaaacgta gacgccattg ccaacccagc cgccagcgcc
    30421 caacgtgcta ttggcctgcg ccataatagt ggtttggcgg gtatatccgg caacgttata
    30481 gctggagata ttattacttg ccgtatttaa cgccgcctgg gccgcgttca gtccgctcat
    30541 ggcgttattg atcaagctgg acatggaggt tccttataag ccttcaatta cagagtatta
    30601 tcggcagcga cccgccggac ttgagttatt cagaacagat tatcaatgtt catgctgtag
    30661 gttttgctca ccttgtcgct tatcgatttc atctgctgga tcatgttggt gagtttacgg
    30721 gcatagtgag gatcggtagc ataccccgcg tcctgtaggg cctgcgcccc ctgctccgca
    30781 ctcgcggctg tcgtcacggc ggcgtagcgc gggttacgtg ttaacagccc aacgtaatcc
    30841 gacaatgctt ccagatacga gctgtagacc cgaaatttcg cttttacttt cttcgcttcg
    30901 ccattttcat attcagtcgt ggtgatttca gtaactggac ctttccagtt gccagaggct
    30961 ttgacgccaa acaggttata gctcggctcg ccgttttcgc ggcggatttg ccgttgcccc
    31021 caaccagact ccagcgccgc ctgagcgagg atcaaatgat gtggcacacc gctttgctgg
    31081 cttgccagtt gggcgggcag cgagagttgc gcgaggaatg ctttactgtc acccggcagc
    31141 gaatcatcgt agttacgtgg cacggccttt tgcaccagct gcgaaagcgc ctgattttga
    31201 taacgcacca cagtttcgag cgggaatttc atcggtgctg ctggcgtgga ctcctctggc
    31261 aatggttgtt ctggcgtcat ctgtttaacc atcatctctg caagccccag acctttgccc
    31321 gccgtcatct gttgggcaat ctgctggtca tacatactgg tatacaggcg agtgtgctcg
    31381 ctgctgaaca ggccatcttt tggtaaagcg tcgcgcatgc ttttcaacat catctgcacg
    31441 aacatccctt ccacctgacg ggccaccgga cggatatttg ccgccggatc ttcgcccgct
    31501 ttcgccttta gttcgttgag tgattgcgca tcccaggccg cacttgccag tagtttgctg
    31561 tcgctgatca tcagatgatt tccagttttg cccgcagaca tcccgcactt tgcattgatt
    31621 gcagtatgga catcagatcc atcggcgtag cgcccagcgc attgagcgcg cgcaccacgt
    31681 tattgaggct ggcgctggaa cgtacgcttt gcagcgaacc gccgctctgg cgtaaatcga
    31741 tctgcgtttg tggagtaacc acagtctgtc caccaccaaa cggtgtatct ggctggctga
    31801 cattggcctg acgattgact gttactgaga gatttccctg cgctaccgcg cagctgtcga
    31861 gggtcacttc gcgattcatc accaccgaac cggtgcgcga gttaatcact actttagcgt
    31921 cctgcggggt gacattaacc tgcatattct ggatatctgc aaggaaacgg acctgggaac
    31981 tgttgccact tggtacgcgc acctgaatag tccgcgcatc taacgcggtg gcgctgccat
    32041 atccacgcac gcggttgatg gtgtcagcga tttgctgcgc catgctgaaa tcttcgtcgt
    32101 taagttgcaa attaagggta ttcccgacgc caaactggct gggcaattca cgttcaataa
    32161 ccgcaccatt ggtgatccgt ccaccgttca gttggttcac ctgaacactg ctaccgccag
    32221 cggaggctcc tgcgccgcca accagaatat tgccctgcgc cagcgcatac acctgactgt
    32281 caacgccctt aagcggcgtc atcaacaacg tacctccacg caagcttttg gcatttccca
    32341 tggaagaaac caccacatcg atggtttgcc cctgacgtcc aaacggagga agtgactctg
    32401 tcaccattac cgcagcgacg ttttttagct gcatattggt gcccgtcgga acggtaattc
    32461 ccagctgtga gagcatgtta ttaagcgttt gtgtggtaaa cggcgtctgg gttgtctggt
    32521 caccggtgcc atccagcccc accaccagac catagccaat cagtgagttt tgccttaccc
    32581 cctgaacact ggtgagatcg cgaatacgct cagcctgagc cgccgtcgta accagtagaa
    32641 gaattaatgc agagagaaat ttaatcacca cagcctcact tacattggcg acaggttaag
    32701 gaagaaacgc tgcaaccagc ccatattttg cgcttcgtta atgtagccat tgcctacgta
    32761 ttcaatgcgc gcatccgcca cctgagtaga cggtacggta ttgctgccgc tgatagtgcg
    32821 tggattaacc acgccagaga agcgaataaa ttcggtaccc tgattaatgg caatctgttt
    32881 ttcacccacc acatgcaggt tgccgttgac cagtacctgg tcaaccgtca ccgtcaacgt
    32941 gccgctaaag gtattgctgg cattggcccc gccctttccg ttgaacgtgt taccaccgga
    33001 ggcttcgaca tcggcacgag cgttaccaaa cagcccctgc aaatagcgcg gcacagtatc
    33061 aaagccaaaa ttagttttac cgtcacggct ggcattcgca gaggagcttt tgctggcgct
    33121 gacgttctcc tgcaacacga tggtcagcgt atcgccaata ttgcgtggtc gacgatcttc
    33181 aaacagcggt tgatagccat agttaatcgg ctgagcagac tggaaaatag aaccgttggc
    33241 gacgggcgtc ggaccgggaa ccggttgtgc actggtcgcc ccctgcacca gcggcgtgga
    33301 gggtatccag gcgcagccgg ttagtgaaag caccaacaag ctggaaatgg cataagtatg
    33361 cgcagcgttt ttttgcatgg ctatcatctt caaaaatcag taaaccggtg aacctgccac
    33421 cggttaagcc ttagagttgc gtcagttttt gcagcatctg atcggtggtg gacaccgctt
    33481 tactgttgat ttcgtaagcg cgttgcacct gaatcatatt gaccagttct tccgccacgt
    33541 tgacgttaga cgtttcaaca tacccttgat acagcagtcc cgcgccgttc aggcccggcg
    33601 tgctttcgtt cggtacacca gaggattgcg tttcggtgta gaggttttcg ccaatgctct
    33661 ccagcccggt gtcattcata aaggtggtga gattgagctg cccaacctga accggagctg
    33721 cctggccttg ttgggttacg ctgaccacgc catcacgacc gatggtgata cttaacgcat
    33781 tcgccggaat ggtgatcgct ggctgcacct gaaaaccacc agctgcccgt tctgatccac
    33841 ctggaaagag ccgtcacggg tataggctga tgaaccatct ggcaacatca cctggaaaaa
    33901 gccctgccct ttaatcgcga catctttgct gttgttggtc tgcgacaggt ttccctggct
    33961 gtgtaagcgt tcagtggcga ccgggcgtac ccccgtgccg atttgtaatc cggagggtaa
    34021 ggtggtttgt tcggaagact gtgcccccgg ctggcgaatg gtttgataaa gcagatcttc
    34081 aaacaccgcg cgctgacgct taaaaccgtt agtactgacg tttgccaggt tgttggcaat
    34141 gacgtccata ttggtttgct gggcgtcaag gcccgttttg gcgatccata atgaactgat
    34201 cataaaatgt cctgtagtgg attagtgaca aagtgcatat gtcttcttgc cggatgcggc
    34261 gtaaacgcct tatccggcct acaagtgctc gcaaaatcga taaattgcga ttgcccccat
    34321 agacccgata aacttgcgca tcgggcaact tatacttgtc atgtatcctt tcaattaact
    34381 catcgacagc agttggttgg cacggcctgc gttatcatcg acgctgctga tcaccttcat
    34441 ctgcatttca aaacgccgcg cgctggcaat catgtcgctc attgccgcaa cggcattgac
    34501 gttactgcct tccagaaccc ccgacatcac acgcaaggtt ggatctgcct gcagtaccgg
    34561 ccctcgcgtg gcctgggttt ctgcgcttaa acgaaaaatg ccgtcgtcac cgcgctgcac
    34621 ttcgctgccc gtggctttca ccagtttcag acgccctact ggcgcaactg tatttgccgg
    34681 atcgcccgga ttgagcgccg agattgtgcc atcggcagca atagtgattt ccgctccttc
    34741 cggcacagca attggccccg cctcgcctat cacctgatgc ccctgaattg tcagttgccc
    34801 ggtgggatca acctgaatgc tgccattacg cgtataccct tcgctgccgt cagcggtctg
    34861 cacggccagc cagccatcct gctgcaacgc gacgtccagc gggcgcgagg tgtaatccat
    34921 tttgccgggc gtcatatctg cgcccggcgt tgacgccgtg accaacgtgc gcgtgggcag
    34981 agaaagccct tccactggca ccgcgcgtaa agcattcaac tgcgcgcgaa aaccgggcgt
    35041 tgaggcattg gccagattac tggcggttac cgcctgttga ttcagtgtct ggctggctgc
    35101 tcccatcgcg gtataaattg cgtgatccat tgagctatcc cgtcagcgat tagcgtaagt
    35161 taaccagcgt gttgaggatc tggtcctgag ttttgatggt ctgggcgtta gactgatagt
    35221 tacgctgggc aacgatcata ttgaccagtt ctttactgag atcgacattg gacgcttcca
    35281 acgcaccgtt ggtcagggtg ccaaagttgc ccgtcccggc tgtccccaac agcgccacgc
    35341 cagaagattg cgtcgcagac cagacgttgt cgccttcgga tgccagacct tcgttgttgg
    35401 caaagttcgc cagtacaatc tgccccagca gttgtgtttg ttcgttggaa tagttgccga
    35461 caaccgtacc gtcatcattg atttgataac tcaccagatc gcccggtttg tagccattct
    35521 gggtggttgc cacaatattg ttagcgccgg tattttgctg catggagttg aggaagctca
    35581 ggctaaacgt ggcgggatct gcgccgttaa ttgcgccagt ggtaatattc tctgttggat
    35641 ttgaggtcag aacgccattg gcattaaaca ccagcttcat tgcaggctcg gctgtacctg
    35701 ttggatcact gctatcctgg gtgtagacct gccagttatt atccccggtc ttcacaaagt
    35761 agacgctcat gtcatgagca ttaccctgac tgtcgaaaac agtcaccgaa ccttttttgt
    35821 tatagctatc cgcattgctg gcgctgaatg gcgtaacagt aggaagcgga tcactggaat
    35881 tcaggttgat ctgcatcgac gccgtggtgg tagttttcgc tgccatcagg gtattcggga
    35941 tcgaaatatt ggtcggattc gccccttgct gaatagtcgg cggcgtaccg gttgccgggt
    36001 aacccgtcag ctgtaaacct tgcatattca ccaggttacg gttttcatcc agcttaaatt
    36061 gtccgttacg gctgtagaac accgaaccgt tgctgtctac cagacggaaa aaaccgttct
    36121 ggctgatagc aacgtccaga cctcgcccgg tgttggtggt cgtgccatcg gtaaagtcct
    36181 gagtgatacc ggcaactttt acccccagtc ccactttcga accggcaaac atatcggcaa
    36241 aagaggccgt gcctgattta aagccgtagg tggcggagtt ggcgatattg ttgccaataa
    36301 catcgaggtt ggtggcggca gcgtttaatc cgctaaccgc ttgagaaaag gccatgactg
    36361 actcctgaaa tgtgaaggct taaattatct gccgtacttc gtcgagggtg gtggtgccgt
    36421 aagtgccgag atccagcgta ttaccgctgt tgccgcggat cacaccctgc accagagcaa
    36481 actgcagcgg ctgggcaacc agttgtgtac caccgttact ggcgctaatc gctacattgt
    36541 aagaaccgtt cggcgcagtg ctgccatcag tcaacgtacc gtcccaggtg aaactgtgga
    36601 ctccggcggt cagttcacca atatcaatgg tgcgcacaac cgcgccattt ttatcggtga
    36661 tggtggccgt aactttgtct gccgcctgtt gcagctcaac accaaacggc gtggtcgtgg
    36721 tcacagcccc ttcttcactg ccggttcccg caagaacagt ggtgccgggg atcatcacgc
    36781 cgtgaccgat caggttactg gcctgtaacg actggctgtt atcaatctgt ccggaaatag
    36841 atccgagcgt ggtattgagt ttttcaatcc cgctgaccgt gctgatttgt gccaattgcg
    36901 acgtcagctc gttgttttcc attggattgg tcgggtcctg gtttttcagc tgcgccacca
    36961 acaaagtcag aaaactgctt tgtaagtctg cggcgttgct gcccgtgagc gaactactgc
    37021 tggtggtact gacgccggta tttgtcggat cggtggtggt taccgcaatg gacatagctt
    37081 tctcctttat tgaccgagcg taagggtttt cagcatcatg cttttcacca tgttgagcac
    37141 ttcaacattg gcctgatagc tgcgtgacgc cgacatggtg ttaaccatct ccccgacgac
    37201 atcaacgttc ggcattttta cgtagccctt tgcatccgcc agcggattac ccggttcata
    37261 aaccagtttg tccggggcct gactttctat aacatcggca acctttacgc cgcctgtcgc
    37321 agcacctggt gcagcgttaa cctggaatac cacctgtttt gcccgatatg gctgtccatc
    37381 gggaccggtc acgctatcag cattcgccag attactggcc gccacgttca agcgctggga
    37441 ctgggcagtt aacgccgacc cggcgatatc aaaaatattc agcagtgcca tccgttaatt
    37501 tccgctctgt aaaacgttca tcatgccttt gatttgcccg ctcaacgcgc taaggctcat
    37561 ctggtattgc aggctgttat cggcaaactg ggtgcgttcg cgatccatat cgacggtatt
    37621 accgtcaagc gaaggctggt ccggaatacg gtattgcagt tctgcggtag gaggcgtcag
    37681 cgcctgcgcc ggaatgtgtt gcgttgaggt catcgtcagt gcaaccacac tggttgcatc
    37741 ccgtccacgt tgcatgactt ttttaagttc actggcaaaa tcgatatcgc gcgcctgata
    37801 accaggggta tcggcattgg cgatgtttgc tgccagcact tcctgacgct gggcgcgcag
    37861 attgagcgcc tcttgttgaa aacgtaaggc ggcgtcgagc ttatcgagca tatctcctcc
    37921 gcaggtatca aaattctgcc atcagcttaa atgcctttac gcgcgcgtta tcggcggaat
    37981 aaacgcaaaa tgggtcgcta tttatgccgt tgatgaccat tgcggacagg tacaattcac
    38041 gttgtagaaa tggctggggg cgaaaatgct ggcaataaaa cgtagcgtgg cgatcatctc
    38101 gatactattc actccgttaa gtgcggcgag taatctcaca tcgcaattgc aaaccttttt
    38161 tagcgcccag ctcgcggggg taagtgatga ggttcgtgtt tctattcgta cagcgcccaa
    38221 tctactaccg ccgtgcgagc agccattgct ttcgatgagc aataattccc gcctgtgggg
    38281 caatgtgaat gtgttggcac gctgcggtaa cgacaaacga tatttacagg ttaatgtaca
    38341 ggccacagga aattatgtgg ttgccgcgat gcccattgtg cggggaggaa agctggaagc
    38401 tggcaatgtc aaactaaaac gcggacggca ggataccctg ccaccgcgta cggtgctgga
    38461 tatcaatcaa cttgttgatg ccgttagcct gcgcgatcta tcacccgatc aacctatcca
    38521 gttaacccag tttcgccagg catggcgggt aaaagcggga caacgcgtca acgtgatcgc
    38581 cagcggtaac gggtttagcg ccaacgcaga aggtcaggcg ctgaacaatg cagccgtcac
    38641 acagaatgcg cgggtgcgca tggtatcggg actggtggtc agcggcgttg ttgatgcaga
    38701 tgggaatatt cttataaacc agtaagctgt taaagattac ccgtcccttg ccgataaata
    38761 agcaacacat gataaaagcg ccctcaatga ggaataaacc atgagtattg atcgcacttc
    38821 gcctctgaag cccgtaagca ccgttcaacc acgcgaaacc actgacgcgt cggtaacgaa
    38881 cacccgggcg gcaaaaacaa cagcctccac cagcaccagt gtgacgttaa gcgacgcgca
    38941 agcaaaactg atgcaacccg gcagcagtga tatcaatctt gaacgtgtcg aagcgttaaa
    39001 actggcgatt cgtaacggtg aactaaaaat ggacaccggc aaaattgccg atgcgctgat
    39061 caacgaagcg cagcaagact tgcagagtaa ctgatcgtat gacacgtctt gcagagatcc
    39121 tcgaccagat gtccgctgtg cttaacgatc tcaaaacggt aatggatcaa gaacagcaac
    39181 atctctctat ggggcagatc aacggcagcc agttgcaatg gattacagaa caaaaaagct
    39241 cactgctggc gacgctggat tacctcgaac agttacgcag gaaagaaccc aatacagcaa
    39301 atagcgttga tattagtcaa cgctggcagg aaattactgt gaaaacgcag caactacgcc
    39361 aaatgaatca acataacggc tggttactgg aaggacagat tgagcgcaat caacaggcgc
    39421 tggaaatgtt gaaaccgcat caggaaccga cgctatatgg ggcgaacggt cagacctcaa
    39481 caacccatcg cggcggtaaa aagatttcga tctgaatatt atctccggcc tgcactgcag
    39541 gccggaatgc attgttacac cgtccggcgg gcaaattctt taactttgaa gcccagtacc
    39601 gccagtgcag cgaagtacgc ggcaatcccc gccagcacga ccgccattaa acgcagtaag
    39661 cgccaaggca tggtacccaa tgaccactcc ggcatgatat gtaacatacc taaaagcacg
    39721 ccagacatca ccagtaccgc caccaccaga cgcaacagaa acgccatcca gccgggttgc
    39781 ggagtaaaga ttttctgctt acgcaactgc cagtaaagca gcgaagcatt cagacacgcc
    39841 gccagaccaa tagaaagtga cagcccggca tgtttcaacg gaccaataaa cgccaggttc
    39901 atcaattgcg ttaaaatcag cgtaacgatg gcaattttca ctggcgtttt aatgtcctgg
    39961 cgggaataaa agccaggagc caacactttc actacaatca ggccaatcaa acccaccgag
    40021 taggcaatta acgcccgctg ggtcatcagc gcatcaaacg cggtaaattt accgtactgg
    40081 aacagcgaaa cggttaacgg acccgaaaga atgcccaacg caaccgcact cggcagcgcc
    40141 aacaggaaac aaagacgcaa cccccagtcc atcaaacggt tgtattcatc atgattgcca
    40201 ctggcaaaac ttttcgacag cgacggcagc aaaatggtgc caagcgccac gcccagcaca
    40261 ccggagggaa actccattaa gcggtcggcg taatacatcc aagacaccga accggaagca
    40321 agaaacgagg caaaaatggt gttgatgatt aaggagatct ggctcacaga gacgccaagg
    40381 atcgccggtc ccatctgttt caccacgcgc attgctccgg catcgtggaa gttaatgcgc
    40441 ggcaggacca gcatgccgat cttcttcagg tgcggtagct gatacaccag ctgcaggatg
    40501 ccgccgaccg taacagccca cgccagcgcc agcaccggtg ggttaaagta cggtgcggca
    40561 aacagcgcga aaccaatcat gctgatgtta agcagtgttg gcgcaaacgc cggaatcgag
    40621 aagcggttcc acgtattcag aatcgctccc accagcgacg ccagggagat cagcaagata
    40681 tagggaaagg taatctttag tagctggctg gtcagggcaa atttgtcagc tgtgtcagcg
    40741 aagcctggcg cggtcaccat gatcacccac ggtgcggcga gcatgccagc gaccgtcaca
    40801 accgccagcg caagtgtcag caggccagaa acataagaga caaagacccg cgtggcgtct
    40861 tcaccctgct tacttttata ttccgccaga atcggtacaa atgccaggga aaatgcccct
    40921 tcggcaaaga tacggcgtaa caagttagga agtttaaaag cgacgaaaaa ggcgtcggtt
    40981 gccatccctg cgccaaagat tctggcgaca attgcgtctc gtgcgaagcc aagcacacgc
    41041 gaaaacatgg tcatcgagct gacggcggcc agcgatttta ataaattcat cggtgttcta
    41101 atccagaccc acgggcaaaa cgcctgcaag gcaggcgctg aaagaggcga atagtctacc
    41161 tggattatgg tgaattgcta ccgccagatg ttacagggtt tattcactca tcgcatcgcg
    41221 ccagatcttg tcaacgatac gttgcgccag cactgcctgt tcgccggcgg tttgcagaac
    41281 tgtctgattt tgcacacatt caatgaagtg ccgcgcacag ccgacaaatc cacgttgctc
    41341 aagagtgctc tgccaaccag gaatcggttt atgcactacg ccctgcccgc gctcctcacg
    41401 ccattcgcgc atatccgtaa tgtcgatgag cgcaccgtca gtcacggcct gcacggtttc
    41461 acgctgactt ccggcacggc gatgcataca ggtggtgatc tgcaaaggat cggccgaaaa
    41521 atggtgctcg gcaaacagca tttcgccagc gtcgttagtc agtagcgtac cgccatccag
    41581 agaggctttg ccgcccgaca accacagcgc ggtatccacc acatgcagat aatcatccag
    41641 caacgtgaaa taaagatcgt gtggcccgac actattgcta cgatgtttat ccattcttag
    41701 cgaagctgcg gtagcgagtt gcgtttttaa ctcaccgtag agtggtgcga aacgacggtt
    41761 aaaaccgacc atcaacgtca gttttttccg cgccgccagt tccaccagcc gttcagcatc
    41821 gcgcagattt tctgccagcg gtttatcgac acagacatgt acacccgcat tgagtaacgt
    41881 actgaccacg tcaaagtggc tggcggtgct ggaatgcaca aaaaccgcat cgcaactggc
    41941 ggcaaggctg gataacgaat cggcataagg aatgcgccag ctttcacaaa ttggcagggc
    42001 tttcgcgcgc gtaggcgacc aggctccttg taacatccag tcagacgctg ccgccagcac
    42061 cggtaaccac gctttttgcg caatgccgcc taatcccaca acgccgatac gtaatttttt
    42121 cactttaatc tcccagatgg gccagcaacg aatcaagacg ctgtttcagt tctgccactt
    42181 cgatttccag ggcttcgacg cgggcctgta aatcaccatc aaccgcgttc gacatatccg
    42241 ttaccgccgg ctgatcctct acctcaccac tgaaaagatg catgtagcgg ctttcgcgtt
    42301 tacccggttc gcgggccaga cgcaccacaa aaggaccatc ttcgcgattt gccagttgtt
    42361 ccagcgtcga ctccacttcc gccatatcgc tgaattcata cattcgcgcg gcgcggctgc
    42421 gcagttcacc cggcgtctgg gcaccacgca ataacaacgt ggtgattaac gccacttccg
    42481 ctgcgctcag tttcagatcg ccaaattctg aattacaaaa acgttgttca tatttggtga
    42541 cccgattacc aaagccactc accgtgcgca gataatggcg tttgaccaga ttatccagtt
    42601 gctcctgcac ttcagattca ctcagattca tgaccggttc acggttcgtt ttctgattac
    42661 aggccgtgac tacaccattg actgagagcg ggtattgctc cggcgtggtc acctgctttt
    42721 ccagcaagca accaatcacg cgagcttcaa gggcagtaag ttgatatttc atgagattct
    42781 ccttagcggc cgggcgtcca gtctggggta gttaatgccg tcagtacgtg atcgcgccat
    42841 tgtccatcaa tcaacagata gtctttcgca tagccttctt tttcaaaacc cagtcgcgcc
    42901 agtaaatcac cgctgcgttt attgtgcggc atataattag ccataatgcg atgaatatgt
    42961 tgggtgcgct gcatataacg aatggctgcg gtcagggctt caaacatgag tcctttgccc
    43021 tgccattttt gcccaatcga ataaccgaga tagcaggcat gaaaagagcc acgaacaaca
    43081 ttggaaaaat tggcaacgcc aataatctct ttttcgtccg ggtcgaataa gccaaagtag
    43141 aaagctgaac cttgtttatg aaattcgtta atcatcccca gcctggcctg ccagcctgat
    43201 ggataacagt ggctttcgtc gcgcactggc tcccagggct tgaggaaatg gcgattctct
    43261 gcgtaataat ccgcaagacg ccaggcatca cgatcatgca ccagacgcac gaccagtcgg
    43321 tctgtggtta agcgcacttt tggcacgtta ctgcgatagc caaacattct atacctactc
    43381 cttcccgtaa cgtctttaat acgctgtaag tattactata cctgcgcaac cagaccctgt
    43441 gaaaagtcca aaatgccatt ttctgcatta ttcacatatt tgataaaaac tctctatcaa
    43501 agcgacgttt tgataaaaaa atattgtccc cgaagtgatg ggaaaagtct tagcggcaat
    43561 gcagaataga aagtgctcat cttttatttc ccgggagggg aaatgtcccg cgtatcgcag
    43621 gcgaggaacc tgggtaaata tttcctgctc atcgataata tgctggtcgt gctggggttc
    43681 tttgttgtct tcccgctgat ctctatccgc ttcgttgatc aaatgggctg ggccgccgtc
    43741 atggtcggta ttgctctcgg tctacgccag tttattcagc aaggtctggg tattttcggc
    43801 ggcgcaattg ccgaccgctt tggtgccaaa ccgatgattg ttaccggtat gctgatgcgc
    43861 gccgccggat tcgccacaat gggtatcgcc cacgaaccgt ggctattgtg gttttcatgc
    43921 ctgctctcgg gactcggcgg cacgttgttt gatccgccgc gttcggcgct ggtggtgaaa
    43981 ctaatccgtc cacaacaacg ttgtcgtttt ttctcgctgt tgatgatgca ggacagtgcc
    44041 ggtgcggtca ttggcgcatt gttggggagc tggctgttgc aatacgactt tcgcctggtc
    44101 tgcgccacag gggccgtttt gtttgtgctg tgtgcggcgt tcaatgcgtg gttgttaccg
    44161 gcatggaaac tctccaccgt acgcacgccc gttcgcgaag gcatgacccg cgtgatgcgt
    44221 gacaagcgtt ttgtcaccta tgtcctgacg ctggcgggtt actacatgct ggctgtacaa
    44281 gtgatgctga tgctgccaat tatggtcaac gacgtggctg gcgcgccctc tgccgttaaa
    44341 tggatgtatg ccattgaagc gtgtctgtcg ttaacgttgc tctaccctat cgcccgctgg
    44401 agtgaaaagc attttcgtct ggaacaccgg ttgatggctg ggctgttgat aatgtcatta
    44461 agcatgatgc cggtgggcat ggtcagcggt ctgcaacaac ttttcaccct gatttgtctg
    44521 ttttatatcg ggtcgatcat tgccgagcct gcgcgtgaaa ccttaagtgc tttgctggcg
    44581 gacgcaagag ctcgcggcag ctatatgggg tttagccgtc tgggtctggc gattggcggc
    44641 gctattggtt atatcggtgg tggctggctg tttgacctgg gcaaatcggc gcaccagcca
    44701 gagcttccgt ggatgatgct gggaattatt ggcatcttca ctttccttgc gctgggttgg
    44761 cagtttagtc agaaacgcgc aacgcgtcgt ttgctggaac gcgacgcctg atttgatact
    44821 cccccctgac tcctccatac tgaaagtgaa ctgacagcca aatggaggag tcacgtgaag
    44881 ctatacattt acgatcactg cccttactgc ctcaaagccc gcatgatttt cggcctgaaa
    44941 aatatccccg tcgaattaca tgttctgctc aacgacgacg cagaaacacc cacccggatg
    45001 gtcggtcaaa aacaggttcc cattctgcaa aaagatgaca gccgctatat gccagaaagc
    45061 atggatatcg ttcactatgt cgataaactc gacggcaaac cgttactgac cggcaaacgt
    45121 tcccctgcca ttgaagagtg gctgcgcaag gtcaatggct acgccaacaa actgctgttg
    45181 ccgcgttttg ccaaatcggc atttgatgag ttttctactc ccgccgcgcg caaatatttc
    45241 gtcgacaaga aagaggccag cgcgggtaat tttgccgacc tgctggccca ctctgacggt
    45301 ctgattaaga atatcagcga tgatttgcgt gcgctggaca aactgatcgt caaaccgaac
    45361 gccgtgaatg gcgaactttc ggaagatgat attcagctat ttccgctact gcgtaatctg
    45421 acgctggtag ccggaatcaa ctggccaagc cgcgttgctg attaccgcga taatatggcg
    45481 aaacagacac aaatcaattt gttatcatca atggcgattt aatcctgacc cgccgcggca
    45541 gcctgtattc gcgggttgcc gccgttttca gttttttctg ctggcgagtt tatcgctggt
    45601 gacacatgct gcgttgtgtc aggatattga cgcagatttc atgtcgagga ctaccatgaa
    45661 caagttttta tttgccgctg cattgatcgt cagtggcctg ctcgttggct gtaatcaact
    45721 cacccaatac accatcaccg aacaagaaat taaccagtcg cttgcgaaac ataataattt
    45781 ctcaaaagat atcggtttac ccggcgtggc tgacgcccat attgttctga caaacttgat
    45841 cagccaaatt ggtcgcgaag agccgaataa ggttacccta accggagacg ccaatctgga
    45901 catgaactcc ctgttcggta gtcagaaagc gaccatgaaa ctgaagctga aagcgctgcc
    45961 cgtgtttgat aaagaaaaag gtgcgatctt cctgaaagag atggaagtgg tcgatgcgac
    46021 ggtacaaccg gaaaaaatgc aaacggtgat gcaaacgttg cttccctatt tgaaccaggc
    46081 attacgcaat tactttaacc agcaacctgc ttacgtcctg cgcgaagatg gcagtcaggg
    46141 cgaagcaatg gcgaaaaaac tggcgaaagg cattgaagtg aagccaggcg aaattgtcat
    46201 tccgtttacg gattaatgac gagggcgcat tcgcgccctt tatttttcgt gcaaaggaaa
    46261 acgtttccgc ttatcctttg tgtccggcaa aaacattcct tcagccggag catagagatt
    46321 aatgactgca ccatcccagg tattaaagat ccgccgccca gacgactggc accttcacct
    46381 ccgcgatggc gacatgttaa aaactgtcgt gccgtatacc agcgaaattt atggacgggc
    46441 tatcgtaatg cccaatctgg ctccgcccgt gaccacagtt gaggctgccg tggcgtatcg
    46501 ccaacgtatt cttgacgccg tacctgccgg gcacgatttc accccattga taacctgtta
    46561 tttaacagat tcgctggatc ctaatgagct ggagcgcgga tttaatgaag gcgtgttcac
    46621 cgctgcaaaa ctttacccgg caaacgcaac cactaactcc agccacggcg tgacgtcaat
    46681 tgacgcaatc atgccggtac ttgagcgcat ggaaaaaatc ggtatgccgc tactggtgca
    46741 tggtgaagtg acacatgcag atatcgacat ttttgatcgt gaagcgcgct ttatagaaag
    46801 cgtgatggaa cctctgcgcc agcgcctgac tgcgctgaaa gtcgtttttg agcacatcac
    46861 caccaaagat gctgccgact atgtccgtga cggaaatgaa cggctggctg ccaccatcac
    46921 tccgcagcat ctgatgttta accgcaacca tatgctggtt ggtggcgtgc gtccgcacct
    46981 gtattgttta cccatcctca aacgcaatat tcaccaacag gcattgcgtg aactggtcgc
    47041 cagcggtttt aatcgagtat tcctcggtac ggattctgcg ccacatgcac gtcatcgcaa
    47101 agagagcagt tgcggctgcg cgggctgctt caacgcccca accgcgctgg gcagttacgc
    47161 taccgtcttt gaagagatga atgctctgca gcactttgaa gcattctgtt ctgtaaacgg
    47221 cccgcagttc tatgggttgc cggtcaacga cacattcatc gaactggtac gtgaagagca
    47281 acaggttgct gaaagcatcg cactgactga tgacaccctg gtgccattcc tcgccgggga
    47341 aacggtacgc tggtccgtta aacaataaaa atcgtagctt cctgttgtca ttaggttatt
    47401 taacctgtat aaataaccag tatattcaac agggggctat tatgcgaatt gaagtcacca
    47461 tagcgaaaac ttctccattg ccagctgggg ctattgacgc cctggctggc gaactttccc
    47521 gccgtattca gtatgcgttt cctgataatg aaggccacgt atcggtacgt tatgccgcag
    47581 cgaataattt atcggttatt ggcgcaacaa aagaagataa acagcgcatt agcgaaattc
    47641 tccaggaaac gtgggaaagc gccgatgact ggtttgtcag cgaataatat gcagtgattt
    47701 ttgtttttgc cgggtcgccc cggctt
//
LOCUS       DAZPDU010000009        46718 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZPDU010000009 DAZPDU010000000
VERSION     DAZPDU010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46718
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.009"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(16..867)
                     /gene="pdxK"
                     /locus_tag="ACHURS_000461"
                     /gene_synonym="thiJ"
     CDS             complement(16..867)
                     /gene="pdxK"
                     /locus_tag="ACHURS_000461"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFR1236281.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            972..1340
                     /locus_tag="ACHURS_000462"
     CDS             972..1340
                     /locus_tag="ACHURS_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFR1236282.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            complement(1343..2254)
                     /gene="cysM"
                     /locus_tag="ACHURS_000463"
     CDS             complement(1343..2254)
                     /gene="cysM"
                     /locus_tag="ACHURS_000463"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFR1236283.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(2388..3485)
                     /gene="cysA"
                     /locus_tag="ACHURS_000464"
     CDS             complement(2388..3485)
                     /gene="cysA"
                     /locus_tag="ACHURS_000464"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFR1236284.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRDRPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPEQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(3475..4350)
                     /gene="cysW"
                     /locus_tag="ACHURS_000465"
     CDS             complement(3475..4350)
                     /gene="cysW"
                     /locus_tag="ACHURS_000465"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFR1236285.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(4350..5183)
                     /gene="cysT"
                     /locus_tag="ACHURS_000466"
     CDS             complement(4350..5183)
                     /gene="cysT"
                     /locus_tag="ACHURS_000466"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFR1236286.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(5183..6199)
                     /gene="cysP"
                     /locus_tag="ACHURS_000467"
     CDS             complement(5183..6199)
                     /gene="cysP"
                     /locus_tag="ACHURS_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFR1236287.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWTKENGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQTRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            complement(6370..7161)
                     /gene="ucpA"
                     /locus_tag="ACHURS_000468"
     CDS             complement(6370..7161)
                     /gene="ucpA"
                     /locus_tag="ACHURS_000468"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFR1236288.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(7290..8147)
                     /gene="murR"
                     /locus_tag="ACHURS_000469"
     CDS             complement(7290..8147)
                     /gene="murR"
                     /locus_tag="ACHURS_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFR1236289.1"
                     /translation="MLYLTKIRNAESEFTENEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVAYEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            8311..9207
                     /gene="murQ"
                     /locus_tag="ACHURS_000470"
     CDS             8311..9207
                     /gene="murQ"
                     /locus_tag="ACHURS_000470"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFR1236290.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGTEDSREGGINDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            9211..10635
                     /gene="murP"
                     /locus_tag="ACHURS_000471"
                     /pseudo
     CDS             9211..10635
                     /gene="murP"
                     /locus_tag="ACHURS_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
     gene            10640..11944
                     /gene="pbp4b"
                     /locus_tag="ACHURS_000472"
     CDS             10640..11944
                     /gene="pbp4b"
                     /locus_tag="ACHURS_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFR1236291.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQIDVGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(12002..12901)
                     /gene="yfeX"
                     /locus_tag="ACHURS_000473"
     CDS             complement(12002..12901)
                     /gene="yfeX"
                     /locus_tag="ACHURS_000473"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFR1236292.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(12997..13572)
                     /locus_tag="ACHURS_000474"
     CDS             complement(12997..13572)
                     /locus_tag="ACHURS_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFR1236293.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(13633..14082)
                     /locus_tag="ACHURS_000475"
     CDS             complement(13633..14082)
                     /locus_tag="ACHURS_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFR1236294.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(14069..14494)
                     /locus_tag="ACHURS_000476"
     CDS             complement(14069..14494)
                     /locus_tag="ACHURS_000476"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFR1236295.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            14708..15577
                     /gene="amiA"
                     /locus_tag="ACHURS_000477"
     CDS             14708..15577
                     /gene="amiA"
                     /locus_tag="ACHURS_000477"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFR1236296.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLPGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            15581..16480
                     /gene="hemF"
                     /locus_tag="ACHURS_000478"
     CDS             15581..16480
                     /gene="hemF"
                     /locus_tag="ACHURS_000478"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFR1236297.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(16486..17538)
                     /gene="eutR"
                     /locus_tag="ACHURS_000479"
     CDS             complement(16486..17538)
                     /gene="eutR"
                     /locus_tag="ACHURS_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFR1236298.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSELVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(17584..18084)
                     /gene="eutK"
                     /locus_tag="ACHURS_000480"
     CDS             complement(17584..18084)
                     /gene="eutK"
                     /locus_tag="ACHURS_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFR1236299.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(18097..18756)
                     /gene="eutL"
                     /locus_tag="ACHURS_000481"
     CDS             complement(18097..18756)
                     /gene="eutL"
                     /locus_tag="ACHURS_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFR1236300.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(18766..19653)
                     /gene="eutC"
                     /locus_tag="ACHURS_000482"
     CDS             complement(18766..19653)
                     /gene="eutC"
                     /locus_tag="ACHURS_000482"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFR1236301.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRTGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(19674..21035)
                     /gene="eutB"
                     /locus_tag="ACHURS_000483"
     CDS             complement(19674..21035)
                     /gene="eutB"
                     /locus_tag="ACHURS_000483"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFR1236302.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNRSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(21047..22450)
                     /gene="eutA"
                     /locus_tag="ACHURS_000484"
     CDS             complement(21047..22450)
                     /gene="eutA"
                     /locus_tag="ACHURS_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFR1236303.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMVLSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(22447..23673)
                     /gene="eutH"
                     /locus_tag="ACHURS_000485"
     CDS             complement(22447..23673)
                     /gene="eutH"
                     /locus_tag="ACHURS_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFR1236304.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATTAKTEAEAQS"
     gene            complement(23990..25177)
                     /gene="eutG"
                     /locus_tag="ACHURS_000486"
     CDS             complement(23990..25177)
                     /gene="eutG"
                     /locus_tag="ACHURS_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFR1236305.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            complement(25167..26003)
                     /gene="eutJ"
                     /locus_tag="ACHURS_000487"
     CDS             complement(25167..26003)
                     /gene="eutJ"
                     /locus_tag="ACHURS_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFR1236306.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQSVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPTVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            complement(26014..27417)
                     /locus_tag="ACHURS_000488"
     CDS             complement(26014..27417)
                     /locus_tag="ACHURS_000488"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFR1236307.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPRIAGLGLGGEGWTTMTITTPTGEGVTSVR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(27429..27716)
                     /gene="eutN"
                     /locus_tag="ACHURS_000489"
     CDS             complement(27429..27716)
                     /gene="eutN"
                     /locus_tag="ACHURS_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFR1236308.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(27823..28116)
                     /gene="eutM"
                     /locus_tag="ACHURS_000490"
     CDS             complement(27823..28116)
                     /gene="eutM"
                     /locus_tag="ACHURS_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFR1236309.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(28155..29171)
                     /gene="pta"
                     /locus_tag="ACHURS_000491"
     CDS             complement(28155..29171)
                     /gene="pta"
                     /locus_tag="ACHURS_000491"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFR1236310.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRALTGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            complement(29168..29971)
                     /gene="eutT"
                     /locus_tag="ACHURS_000492"
     CDS             complement(29168..29971)
                     /gene="eutT"
                     /locus_tag="ACHURS_000492"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFR1236311.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKPPLTVKQIQQRLGETQ"
     gene            complement(29968..30669)
                     /gene="eutQ"
                     /locus_tag="ACHURS_000493"
     CDS             complement(29968..30669)
                     /gene="eutQ"
                     /locus_tag="ACHURS_000493"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFR1236312.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEARKVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAQPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(30644..31123)
                     /gene="eutP"
                     /locus_tag="ACHURS_000494"
     CDS             complement(30644..31123)
                     /gene="eutP"
                     /locus_tag="ACHURS_000494"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFR1236313.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(31136..31471)
                     /gene="eutS"
                     /locus_tag="ACHURS_000495"
     CDS             complement(31136..31471)
                     /gene="eutS"
                     /locus_tag="ACHURS_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFR1236314.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIVGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(31765..34044)
                     /gene="maeB"
                     /locus_tag="ACHURS_000496"
     CDS             complement(31765..34044)
                     /gene="maeB"
                     /locus_tag="ACHURS_000496"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFR1236315.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            34333..35283
                     /gene="tal"
                     /locus_tag="ACHURS_000497"
     CDS             34333..35283
                     /gene="tal"
                     /locus_tag="ACHURS_000497"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFR1236316.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQYAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            35303..37306
                     /gene="tkt"
                     /locus_tag="ACHURS_000498"
     CDS             35303..37306
                     /gene="tkt"
                     /locus_tag="ACHURS_000498"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFR1236317.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKDIYHAWDAREKGEKAQQRWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPLLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEITRGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(37402..38445)
                     /locus_tag="ACHURS_000499"
     CDS             complement(37402..38445)
                     /locus_tag="ACHURS_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFR1236318.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAALAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(38571..39146)
                     /gene="nudK"
                     /locus_tag="ACHURS_000500"
                     /pseudo
     CDS             complement(38571..39146)
                     /gene="nudK"
                     /locus_tag="ACHURS_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
     gene            complement(39214..41193)
                     /gene="aegA"
                     /locus_tag="ACHURS_000501"
     CDS             complement(39214..41193)
                     /gene="aegA"
                     /locus_tag="ACHURS_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFR1236319.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHLTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRLVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            41399..43099
                     /gene="narQ"
                     /locus_tag="ACHURS_000502"
     CDS             41399..43099
                     /gene="narQ"
                     /locus_tag="ACHURS_000502"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFR1236320.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSSVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            43263..46376
                     /gene="acrD"
                     /locus_tag="ACHURS_000503"
     CDS             43263..46376
                     /gene="acrD"
                     /locus_tag="ACHURS_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFR1236321.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGIGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(46475..46534)
                     /gene="ypfM"
                     /locus_tag="ACHURS_000504"
     CDS             complement(46475..46534)
                     /gene="ypfM"
                     /locus_tag="ACHURS_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFR1236322.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT        10684 a        13148 c        12110 g        10776 t
ORIGIN      
        1 catcggcgcc attttttatg cttccgccag cggcggcaaa atcaattcat cgctctcatg
       61 ctgctgggtg tagcgcatca cttccagtac gcgcaacccc gcacggtgca ccgcatcgtt
      121 taacgccttc cctttcagca aaccactgat aagctgagca caaaacaggt cgccagtccc
      181 tttcaggtcg gtttttaccc gtgaatggga aatgacattc acgctgtcgg cactgaccac
      241 caccacctgc atctcctgat tttcttcatt accggaggcg ctggtaatca ccacccattt
      301 taatgtgtct gaaagcagac tttttgcggc agcaatggca ctgtcgagat cgcggcaatt
      361 tttaccggtc aggatttcca gctcaaagat attgggggta attccttgcg ccagcggcag
      421 taaatattgt cgatacgcct cgggaaggtc aggtttgaca taaattccgc tatcaatatc
      481 gccaatcacc ggatcgacca tgatcaatag gtcaggatgg tctttgcgta gcgcagtcag
      541 ccactcggca aggattttga tttgcgatgc cgttcccata tagcccgtgg ttacagcacg
      601 aagttggcgc agcgcatcac gctcctgaag cgcacgcaaa tagccgctaa accattcgtc
      661 cggaatcgca ccaccgtaga aagtgtcata atgcggcgta ttgctcagca ataccgtcgg
      721 cacggcaaag acattcaagc cgttctgttt tatagcaggc accgcaatgc tgttgcctac
      781 gctgccgtac actacctgcg actgcacggc gacgatatcc gcctgcagcg ccctactctt
      841 atcgttaaac aacaacaaac tactcattta attttttctc cttgccgatg atcctcatcg
      901 tattccaacc gaaactttac ctgattctgg cagtcaaatc ggctatcaca aaacaaggat
      961 aaggtaattc aatgaagaaa atcatttgtc tggtcattac actactaatg acactccccg
     1021 cttacgcgaa gttaactgtt catgaagaag cccgcatcaa cgccatgctg gagggattag
     1081 cacagaaaaa ggatttgata tttgtgcgca acggtgatga acatacctgc gatgaagcgg
     1141 tttctcatct gcgtctgaag ctcggcaata cccgtaaccg cattgacact gccgagcagt
     1201 ttattgataa ggttgcttcg tcgtcatcga ttaccgggaa gccgtatatt gtgaagatcc
     1261 ccggtaagag cgatgagaac gcacagcctt ttttacatgc cttaattgcg cagacggata
     1321 aaacggtgcc tgcgcaataa tcttaaatcc ccgccccctg actaaaatgc tcttccccaa
     1381 acaccccggt ggaaaggtag cgatcgccac gatcgcagat gatcgctacc accaccgcac
     1441 cagggttagc ttttgccacc cgcagtgctc cggcaaccgc gccgccggag ctgacgccac
     1501 agaatattcc ttcccgcact gccagttcgc gcatggtgtt ttctgcatcg cgctgatgaa
     1561 tatccagcac ctcatccacc agagaagcgt tgaaaatccc cggcagatat tccgcaggcc
     1621 agcggcgaat gccgggaata ctgctgcctt cttccggctg caggccaaca atcgttactg
     1681 gtttggattg ttcgcgcata aagcgtgaaa cgccggtgat agtgccggtt gttcccatgc
     1741 tggagacaaa atgggtaatg cgcccgccgg tttgctgcca gatttccggc ccggtagtgg
     1801 tgtaatgggc gtaagggtta tcgggattat tgaactgatc gagcagcttt ccttcgccac
     1861 gattcgccat ctccagcgcc agatcgcgcg caccttccat gccctgctct ttggtgacaa
     1921 gaatcagttc cgcaccataa gcacgcatcg ccgcacggcg ttcctggctc atgttgtcgg
     1981 gcatcagcaa tttcatgcga tagcctttca gcgcggcaat cattgccagc gcaatgccgg
     2041 tgttaccact ggtggcttcg attaagacat cacccggttt aatttccccg cgcttttccg
     2101 cctcgacgat catcgaaagt gccgcacgat ctttcaccga acctgccggg ttattgcctt
     2161 ccagttttaa ccacacttca ctgccgttat ccggccccat tcgctgcaac ttcaccagag
     2221 gcgtattgcc tattgtttgt tctaatgtac tcacgatctc tatccatacg tggtgttgcc
     2281 tgatgcgacg cttgcgcgtc ttatcaggtc tacaggttac aaaccttgcc attaaaaagc
     2341 ccggatcgcg ggaagcgcct ccgggcgttt aacattcact caacctatca ggcgctttgt
     2401 gcgagagcaa gttcctcatc gcgggtttcg atacgctcgt cgccgttata cagccgcgca
     2461 tgttgcagac caacgaataa acgctcgcca cgctgcgggg catcgtcgcc atgcatcacg
     2521 accgtcagcg gttcgttgta ccaccccagc ggctgcacca ctaattgggt gtagtgacct
     2581 ttcgggctgg cttccagtac ctgtaccggc agcggcgaat cgagactggt acggcggctg
     2641 atatccactt cccacgggcg caggaagaga tccaccggcc cctgataagc aggtgtgtag
     2701 cccagcggcc agcgatgtgc gccaacatgg aactgcccgc cgcgaatggt tccctgcagg
     2761 cggttcactt cgcccataaa ttcgagcaca aaacgggtcg ctggttcgcg ccatacctgc
     2821 tccggcgcat cagcctgttc aatattgccc tggctcatca ccactacacg atcagctact
     2881 tccgtcgctt cttcctggtc gtgggtcacg aacacgctgg tgaatttcag ctcttcatgc
     2941 agttgacgca gccagcgacg cagctcttta cgcacctgcg catccagcgc gccaaacggt
     3001 tcatcaagca gcagaatttg cggttctaca gccagcgcgc gcgccagcgc cacgcgctgt
     3061 ttctggccgc cggaaagctg tgccggataa cgatccgcca gatgagcaag ctgcaccatc
     3121 tccagcaatt ttgtcacttt cgctttgatg gctgcggcat tcgggcgatc gcgacgcggc
     3181 agcaccgtca ggccaaaagc gatattgtcg aataccgtca tatggcggaa cagcgcgtaa
     3241 tgctggaaca cgaaaccgac cttgcgatcg cgcgcgtgca ggcggctgac gtcggtgccg
     3301 tggaagcgaa tatgcccgct ggtttgatgc tccagcccgg cgataatgcg cagcagcgtg
     3361 gttttcccgg aaccggacgg ccccagcaac gcgaccatct gacctgaagg aatatccagt
     3421 gagatatcgt tcagcacctg ggtgcgacca aacgacttct taatattggc aatctcaatg
     3481 ctcatgatgt tcctcctgct gtgcgcgttt ttcctgattc tccaggcgcc actgcaacat
     3541 actttttaaa aacagggtga taatcgccat cagcgttaac agtgccgcag cggtaaagga
     3601 gccgacggtg ttgtagtcct gctccagcaa ttcaatctgt aacggcagcg acagggtttc
     3661 gccgcgaatc gagccggaaa ccaccgacac cgcgccaaac tcgccaattg cgcgggcgtt
     3721 ggtcaacacc acgccataaa gcagcgccca gcgaatgttc ggcaatgtga cgcggcgaaa
     3781 catctgccag ccagacgcgc caagcaaaat cgccgcttcg tcttcctggc tgccctggct
     3841 taacatcacc ggcaccagct cgcgcaccac aaacggacac gtcacaaaga tggtgaccag
     3901 caccattccg ggccaggaga acataatttg cagattatgt tcatccagcc agccgcccag
     3961 cgggccgtta gagccgtaga acagcagata caccagaccg gcaaccaccg gcgatacggc
     4021 aaacggaatg tccagtagcg tcagcagtaa ctggcgtcca gggaagttaa agcgcgtcac
     4081 cagccaggcc agcagaatgc cgaacaccag gtttaccggt acggcaatca gcgcgatcat
     4141 caccgtcagc cagatggcgt gcagcatgtc cggatcggcc agattctgta aaaccggcat
     4201 cagccctttg ctgaacgcct gtacgaagat ataaatcatc ggcaccagca ggatgaacgc
     4261 cgataccagc atcccgatgc caatcagaaa ccatttgccc cagttaatcg ggcgcgcgtc
     4321 ataacgcttc aattgggtaa cttccgccat taatgaccta ccacacgccg accaaagcga
     4381 ctttgcagag tgttaattga gaacagcagc agcagagatg ccgcgaggat caccgaagca
     4441 atcgcgctcg ctgccgggta atcaaactcc tgtaagcgca caaaaatcat cagcgacgtc
     4501 acttccgtct tccacgcgat atttccggcg ataaaaatca ccgcgccaaa ttcaccaaga
     4561 ctacgggtaa acgacagcgc cacgcccgcc accagcgccg gagaaagttc cggcagcacc
     4621 actttgcaga aactctgcca gcgcgttgcg ccaagcgttt ctgccgcttc ttcatattcc
     4681 gggcctaact cttccagcac cggctgcacg gtacgtacca caaacggaat gctggtaaag
     4741 gccatcgcca ccgcaatccc cagccaggta taggtgactt tgatatcaaa cttcgccagc
     4801 cattcaccgt aaaaaccgtt tacagaaaag agcgaggcta gcgttaaacc ggcgactgcc
     4861 gttggcagcg caaagggtaa atccatcagc gcatcaagca gcgtgcggcc cggaaagcga
     4921 tagcgggtta agatccacgc catcagcagg ccaaaaacgc cgttaaaaat cgatgccaca
     4981 aacgctgaca gcagcgttac tttgtaggcc gctactacct gcgggttggt gatcacctcc
     5041 cagtactgcg cccagctcat ctgagccagc tgcatcacca gcgcggagag cggcagcagc
     5101 aaaatcaggc acacaaacag cagactggtg ccgaggctta aggtaaagcc cggcagcacg
     5161 cgtcttgatg aaactgcaaa cattaattac gccccgccgc taacagcttg tctaactcgc
     5221 cgccgctggt gaagtgggtt ttcatcactt ccggccagga gccaaatttg tcttccacgc
     5281 ggaacagctc ggtctgcggg aatttgtctt tcagtttatc catgacttcc gggttattca
     5341 cgcggtagta atagtcggtg atgatggttt gcgcctgcgg gctgtagagc cagttaaggt
     5401 aggctttggc ggctttttcc gtaccgttgg cctgcacgtt tttatcaacc cacgccaccg
     5461 ggaattccgc cagaatgttg gttttcggaa tcaccacttc aaaaccctgc gcttcatact
     5521 gtttacggat gttgttcact tccgactcga agctgatcag cacatcgccc aggccgcgct
     5581 cggcaaaagt ggtggtcgcg ccacgaccgc cagtatcgaa cacttcaacg tttttcagga
     5641 actgggtcat aaactgttcg gttttggctt tatcgccacc gtcagcttta tccgctgcgc
     5701 cccatgccgc cagataggtg taacgcgcgt tacccgacgt tttcgggttc gggaaaatca
     5761 gcttcacatc ggagcgcacc aggtcgttcc agtcgtggat attcttcggg ttgcccttac
     5821 gcaccaggaa gcccatggtg gagtagaatg gcgagctatt atttggcagg cgcgtttgcc
     5881 agtccgccgg gatcagcttg cctttgtcat gcaggatttg tacgtcagtc acctggttat
     5941 aagtgacaac gtctgctttt aagccctgca aaattgccag cgcctgtttt gatgacccgg
     6001 catgagattg ttttattgtc agtttgtcgc cgccgttttc tttggtccat tgttgctcaa
     6061 acggcgggtt cagggcggca aacagctcgc gggagacgtc ataagaactg ttcagcagtt
     6121 ccgttgcctg tacatggccc gccagcagca gagaagcgac cagcgcgagt gagttctttt
     6181 tcagtaagtt aacggccatt gcgcaccctt ataaatttaa tgactttcta atagccatca
     6241 tatttataac ggacgtgaaa ggagtaacgg ttttatatac cgtttggtga tttggaagtt
     6301 gaaaagggaa taagaaccgg gtttaaaacg aaatcctccc cacaaatgca gggaggaaac
     6361 agaggtgaat cagataccga cgctaaccgt ctccggcagt gtgctgccgc catcaatcac
     6421 attctgtgta ccggttaaat agctggattc atccgatgcg aggaaggccg ccagttcgcc
     6481 gacttccagc ggatcggcga ggcgacgcag cgggattgct ttcgccattt cagtcagcac
     6541 cgactctgga tcttccgggt tcgactggcg ggcaatgctt tccgccattg gtgtgcgcac
     6601 gtatcccggg caaatggcgt taacgcgaat accagactgc gcgtactcca ccgccagcga
     6661 ttttgtcagg ccaacaatcg ccgctttcgt taaggcgtac gccgtttcgc caggatcggc
     6721 caccatatca ccagtgactg aagacatcat cacaatgcga ccatctttgc gggcaatcat
     6781 ctccggcagc accgccttcg tgacgttcca tacgccttta atattgatgt caatatggaa
     6841 atcgcgatcg tcatcgctca tatcgaggaa actgcccaga cgacaaacgc ctgcgttatt
     6901 caccaggata tcaatgcgcc cttctttttc cttcgcgcgt ttgatagctg cggctaccga
     6961 cgccgggtca cgcacatcgg cgacaaccgc cgtacagcga tgaccacgac cacacagttc
     7021 gtccgccagc ttttcgatct caggggagat atccagcaag attaggtttg cgccatgacg
     7081 cgcaaaagtt ctggcaattc cttcgccaat tccctgcaat gcgcccgtaa tcagtgctgt
     7141 cttgcccgtg agtttaccca ttttaatgct ctccttgtta atacagagcg ttacactcca
     7201 ttccctctga aaatacagga taaacaaaat attgctgcgg atcagctcac taattcagga
     7261 aaatctcaat ccagtcgctg ctttatgcat catttcaggc gttgtgttag ctcactgctg
     7321 cgctgaatca ttttcaatga ctccacatca ttgagttgca caagaccaac aaacagtaaa
     7381 tcggtaacag aattttgcgc cgtgcgggta gacatcgacg agctacgcca ttcggtttca
     7441 ccggaaacgg tatcgagcgt gaaatgcgcc agccgccgca gcggagagtc agccagagaa
     7501 gtaatggcaa tcaccgttgc cccctgcttt cgtgccgctt ctgcgcacaa gacaatctct
     7561 tttttactgc cgctgtaaga gatagcgatt tgtacatcgc cttttttcaa cgcctgggaa
     7621 acggtggctt gtacgtgtgt gtcggcttca taggccacgc gatagccaat tttcatcagt
     7681 ttgaacgaca gatcgcgccc caccagcgcc gacccgccca ggcccgttat ctggataaac
     7741 ggtgctttac tgatgacctc aatgattttt tgcagccgag cgtagtcgaa caacgcacag
     7801 gtttgctcca gcgccagctc tttttcgcga ttcagtttgc gggcaatcac ttccagcgaa
     7861 tcatcactgg taatcgaact gtgcaggtgc agcgccgtgg cattcgtttt ctcccggctg
     7921 gcactgtatt cgccaattaa cgccatacgc aattcagtaa atccttgcgc gccgagcttc
     7981 tgggcaaatt tcacaatgct cgactgactg atgccgagct gtttcgccat cttgcgcgaa
     8041 gagacggatt tcagctcact cacgttggcc cgcagaaagt cagcaatttt ttgctcgttc
     8101 tcggtaaatt cactttccgc attgcgtatt ttcgtcaggt acaacatctt ccactctccc
     8161 gggctggcag caaaaattcc gcttaagttc ttatgacgct cttcacactc tgcaagtgta
     8221 gactcaatga ttcctttaag tctgtcaatt cgggatatat gattccatac atctgcaatt
     8281 atttgatcgt aaatagtaag gtcaccaccg atgcaacttg aaaagatgat tactgaaggc
     8341 tcgaacaccg cctcggctga aattgaccgc gtatcgacgc tggaaatgtg ccggattatc
     8401 aacgatgaag ataaaaccgt accgcttgcc gttgagcgcg tcctgccgga tatcgccgcg
     8461 gcgatcgatg ttatccacgc ccaggtaagc ggcggcggtc gtctgattta tctcggtgcg
     8521 ggaacatccg gtcgtctggg gattctggat gccagcgaat gtccgcccac ctacggcgtg
     8581 aaaccgggtc tggtggttgg tttgattgct ggcggcgaat atgccattca gcacgcggtg
     8641 gaaggcacgg aagatagccg agaaggcggc attaacgatc tgaaaaatat aaatttaacg
     8701 gcacaggatg tggtggtcgg tattgccgcc agcggtcgta cgccgtatgt gattgccgga
     8761 ctggaatacg cccgccagct cggttgccgc acagtgggaa tttcctgtaa tccggggagt
     8821 gccgtttcca ccaccgccga gtttgccatt acaccgattg taggtgccga agtggtcact
     8881 ggttcttcgc gaatgaaagc aggcacagcg cagaaactgg tgctcaatat gctttccacc
     8941 gggctgatga ttaaatccgg caaagtgttc ggcaacctga tggtcgatgt ggtcgccacc
     9001 aacgaaaaac tgcatgtgcg ccaggtcaat atcgttaaaa acgccaccgg atgtaatgcc
     9061 gaacaagcgg aagcggcgtt aattgcctgc gagcgcaact gtaaaacggc cattgtgatg
     9121 gtgctgaaaa atctcgatgc cgcagaagct aaaaaacgcc tggatcaaca cggcggcttt
     9181 attcgtcagg ttttagacaa ggaataaccc atggccaaag agatcagcag tgaacttctg
     9241 aacaccattc ttacccgtgt cggcggactg ggaaatatcg ccagttgtgg taactgtatg
     9301 acgcgcctgc gtctgggtgt acatgacagt tcactggttg atcccaatat caaaacgctg
     9361 gaaggcgtga agggcgtcat tttgaccagc gaccaggtgc aggtcgtttt tgggcctggt
     9421 aaagcgcatc gtgccgcaaa agcgatgagc gagctgctgg gcgaggcacc ggtacaggat
     9481 gccgcagaaa tcgccgccca gaacaaacgt cagttaaaag ccaaacaaac ctccggcgtg
     9541 caacaatttc tcgccaaatt cgccaccatc ttcacgccgc tgatccccgg ttttattgcc
     9601 gccggtctgc tgctggggat agcgacgtta attgccacgg tgatgcacgt tccggcagat
     9661 gctcagggca cgctacccga cgcgctgaat tttatgaagg tgttcagcaa aggtttgttc
     9721 actttcctgg tgattctggt gggctataac gccgcccagg catttggcgg cacgggcgtc
     9781 aatggcgcaa ttattgccgc actctttttg ctcggttata accccgctgc aaccaccggt
     9841 tactacgccg gttttcacga tttctttggt ctgcccatcg atccgcgcgg caatattatc
     9901 ggcgtgctga ttgccgcgtg ggcctgcgcc cgcattgaag gcatggtgcg ccgctttatg
     9961 tcggacgatc tcgacatgct gctgacctcg ttaatcaccc tgctgatcac cgctacgctt
    10021 gcgtacctga tcattatgcc gctgggtggc tggttattcg aaggtatgtc atggctgttt
    10081 atgcacctga acagtaatcc gttcggttgt gcggttttag ccgggctgtt cctgatcgcc
    10141 gtggtatttg gcgtgcatca gggctttatt cctgtttacc tcgcgttaat ggacagccag
    10201 ggattcaaca gcttatttcc catcctttca atggcaggcg cgggccaggt gggcgcggcg
    10261 ctggcactct actggcgggc gcaaccgcac agtgcgctac gcagtcaggt acgcggggcg
    10321 attattcccg gcctgctggg cgttggtgaa ccgctgattt acggtgtcac cctgccccgc
    10381 atgaagccgt ttgttaccgc ctgtttaggc ggcgcggcgg gcggtttgtt tatcggcctg
    10441 atagcctgat ggggtctgcc gatgggctta aacagcgcct ttgggccgtc tggtctggta
    10501 gcgctgccgc tgatgacttc cgcacaaggc atccttccgg ccatggcggt ttatgctggc
    10561 gggattctgg tggcatgggt ttgcgggttt attttcacca cgctctttgg ctgccgtaac
    10621 gtcaatctgg actgatatca tgaaacggac aatgctctat ctttctctgc tggctgttag
    10681 ttgtagcgtc agcgccgcaa aataccctgt tctgacagaa agctcgccag agaaagcagg
    10741 gtttaacgtc gaacggctta accagatgga tcgctggatt agccagcaaa ttgatgtcgg
    10801 ttatcccggc gtaaacctgc tgatcattaa agataatcag attgtgtatc gcaaagcctg
    10861 gggagcggcg aaaaagtacg atggcagcgt gttgatggaa cagcctgtca aagccaccac
    10921 cgggacgctg tatgacctgg cctcaaacac caaaatgtac gccacgaact tcgccctgca
    10981 aaagctgatg agcgaaggca aactgcatcc tgacgatcgg attgcgaaat atattccggg
    11041 gtttgccgat agtccaaatg acaccattaa aggcaaaaac accctgcgga tttctgacct
    11101 gctgcatcac agcggcggtt tcccggcaga tccgcaatac ccgaataaag cggtcgcggg
    11161 cgcgttatat tcccaggata aaggccagac gctggagatg atcaagcgca cgccgctgga
    11221 atatcagccc ggcagcaaac atatctacag cgatgtcgat tatatgctgc ttggatttat
    11281 cgtcgagtcg gttaccggtc agccgcttga ccgctatgtt gaagagtcga tttatcgccc
    11341 gctcggcctg acacatacgg tatttaaccc gctgctgaaa ggctttaaac cgcaacaaat
    11401 tgccgccacg gaattgaacg gcaatacccg cgatggcgtg atccatttcc cgaatatccg
    11461 cacctccact ctctggggtc aggtgcacga tgaaaaagcc ttttattcga tgggcggcgt
    11521 ttccgggcac gcaggtttgt tttccaatac cggcgatatt gcggtgttaa tgcaaacgat
    11581 gctgaacggc gggggctatg gcgatgtgca gctgttcagt gcggaaacgg tgaagatgtt
    11641 caccaccagc tctaaggaag atgccacttt tggcctcggc tggcgcgtga atggtaatgc
    11701 caccatgacg ccgacgtttg gcacgctggc aagcccgcaa acctacggtc acactggctg
    11761 gaccgggaca gtaaccgtta tcgatccggt caatcatatg gcgattgtga tgttaagcaa
    11821 caagccccat tcgccagttg ccgatccaca aaaaaatccc aatatgttcg aaagcggtca
    11881 gttaccgatt gcaacttatg gttgggtagt tgatcaggtg tatgcggcgt tgaagcaaaa
    11941 gtaataaaaa ggccagtcag ttttgttact ggctggcctt taatcatcga atcagaaacg
    12001 cttacaacgc cattaacttg tccagcgacg gcgcgaagta atagccgccg gttaccggtt
    12061 tggtgaaacg caacatcgca tcacgcttac catcggtatc gccaaacatg ctcagcagtt
    12121 gctgctcaat gttatgcaga cgcgcgcagt aggcgcagaa gtacagaccg tgagtgccac
    12181 tggcggtgcc gtacggcagg ctctggcgaa caatcttcag ccctttgcca tcttctttca
    12241 gatcaacgcg ggtgaggtga gaggtttccg gacgttcgtc gccgtcaatc tcttcgttgg
    12301 cctctttggt gcgcccgatc atcatctcct gatcgtgaac gctcatccgg ttgagctgct
    12361 tcaggttgtg ctcccaacgc tgcacaaaca catagctgcc gccggcatcc acgccgtctt
    12421 tgataaccgc cacttcgcga cgcgtctctt cacccgccgg gttttctgta ccgtcaacaa
    12481 agccgctcag gtcacgctct tccacccaac ggaagccgtg gatctcttct ttcacttcaa
    12541 tgcagtcacc aaaggcttcc atcgccgcct gggcgacaga gaagtttacg tcgtgacgca
    12601 gagaaagaat gtggatcaac acatcgaact gggtggtcgg cgcaaggcct ttaccgtagc
    12661 ccggaaaatc tttcagctct tctgccccaa cgccgccgct cagagcgcgc caggtgttgt
    12721 taccaaaggc aaccaccgca ccaagatgcg cgtccgggaa tttcgcttca aaagttgcca
    12781 gtttgtcggc aaatgtttta ctggccgcac gcagggcgtc aacttcccct ttcacgttgg
    12841 cttcgatcca aatcgccgcg cggcaatgtt ctggcaaaat gccactctga acctgagaca
    12901 ttgttcctcc tgaaaataag aatgccacgc aatccgtggc attgttgagc gctattttac
    12961 gtgttttttc gcggcggcgg tttgttcaga cgcaaattaa cgccgccaga taattttact
    13021 gactttccag tttttcaggg tatcgtccga aggcattaac ccttcaggac cacgccattc
    13081 cccgctgaac tggtagctaa tatgttgact gccttcggct ttacattcga cggcacgatt
    13141 atcatcacca tcagcttttt ggcaattgcc aaatgctttg ctgtaaaggt cgctaaacgg
    13201 tgtaccgatt ttaacaccgg tgtcagcagg aatatcgcta tccagcacgt caatgcgact
    13261 gatcgtgcct tgatcgccgt taatcaccat cgccacgttg tcgcctttca tcacttcaaa
    13321 aaagcgcacc acgttgccgt tcgcggtttt cattccgctg cgcaggcgat aatcgccatc
    13381 aagcgcatca gcaatggctt gttcttgcag tggtgtggac gccgttaatt cacccacgcc
    13441 ctgctcgctc actttggtgg acgatccaaa ccagttccac gggttagcgg ccgaccagtt
    13501 aactgacgac atcgtggaac agccggtcag catcaacggc atagcgcata acattaaacg
    13561 cagcgatttc atgtcacttc ctttggttat tcaataacgt tgcttggagt gcaaattcac
    13621 caaaaagtgc cgttattctt tttcttcatt aaaacaagcg cgtaaacgtc gattggtcag
    13681 cagccagatt aacgccacaa tatccgccac taccagcgcc agaccgatac cgctaacgga
    13741 ttcaccgttc agccacagcc acggttgcca gcaaagtaaa accacctgcg ccaacagcaa
    13801 cagaaaatag agcacatgcc aagtgcgagg aaatgtagcc cgccgaccgc tcaacaaaaa
    13861 cgccagcacc gccggaatgc ctggaatcag ccccagccag aaattatcgt gatcgggata
    13921 aaacagattt agcagcgcag taccctgctc gcgcgacgca ccggcaatga caaacagcac
    13981 ccaggttcgc gcctgaagca atagcacaag ccagaagagc aagggtaaac gcaggcgtcc
    14041 gtgcgcatca taatggacag gatggaactc agtactcttc atcttcaatc aaacgcttac
    14101 ccagactcag cacgtcggcg tgttcatatc ccaggcgttc atacattccg agcaccatgt
    14161 cgttatcttc cggcacattg atctgaattt tcgggcagcc acgagcaatc agctttttct
    14221 ccagccgatt aagcaacgca ttggcaatcc cacgcccacg aaactctgga tgcacgccaa
    14281 gataataagc agacccgcga tgcccgtcat aaccgcccat caccgttccg accacttcac
    14341 cgtttacctc agcgaccaaa aacaaactga cgtcatggtt catcttacgc tcgatgtcca
    14401 tttccggatc gttccacgga cgcagcaagt cgcaacgctc ccaaagggtg atgacctctt
    14461 cgaaatcttc ctggcgaaat acgcgtatct ccatggtatt cgttaccttt ttgcgggtta
    14521 aaaggctgat tatggcgtga acggtcgaat tagccaatat ctgacgaaaa tcggttgaaa
    14581 aagtggcata atggggagtt gtcaactatt gaaatgaaaa gtaaaacaat tctcaacatc
    14641 aaaccgtcgt aacggattac gcgatacgat ataacatctg gaactttatt attacaactc
    14701 aggccgtatg agcactttta aaccactaaa aacactcact tcgcgccgcc aggtgctgaa
    14761 agccggtttg gctgccctga cgttgccagg aatgtcgcaa gccatcgcca aagacgaacc
    14821 tttaaaaacc agcaacggac acagcaagcc gaaagccaaa aaatctggcg gcaaacgtgt
    14881 cgttgttctc gatccaggcc acggcggaat tgataccgga gcgatcggac gcaacggttc
    14941 gaaagaaaaa catgtggtgc tggcgattgc taaaaacgtc cgttccattt tgcgtaatca
    15001 tgggattgat gcgcgtttaa cgcgttctgg cgatacgttt atcccacttt acgatcgcgt
    15061 tgaaatcgcc cataaacatg gcgcagatct gtttatgtca attcatgccg atggctttac
    15121 caacccgaaa gctgccggtg cttcggtatt tgccctctct aaccgtgggg caagtagcgc
    15181 aatggcgaaa tacctgtctg aacgcgaaaa ccgcgccgat gaagttgccg gtaaaaaggc
    15241 gactgacaag gatcacctat tgcagcaagt gctgtttgat ctggtgcaaa ccgatactat
    15301 taaaaacagc ctgacgcttg gctcgcatat tctgaaaaag attaagccgg tgcataaact
    15361 gcacagccgc aacaccgaac aagccgcgtt tgtggtgttg aaatcgccgt cggttccttc
    15421 ggtgctggtg gaaacctcgt ttatcaccaa cccggaagaa gagcggctgt taggcacggc
    15481 ggcgtttcgt cagaaaatcg ccacagcgat tgctgaaggc gtgatcagtt atttccactg
    15541 gttcgacaac cagaaagcac attcgaaaaa gcgataagtt atgaaacccg acgcacacca
    15601 ggttaaacag tttctgctca accttcagga tacgatttgt cagcagctga ccgccgtcga
    15661 tggcgcagaa tttgtcgaag atagttggca gcgcgaagct ggcggcggcg ggcgtagtcg
    15721 ggtgttgcgt aatggtggtg ttttcgaaca ggcaggcgtc aacttttctc acgtccacgg
    15781 cgaggcgatg cctgcttccg ccaccgctca tcgcccggaa cttgccgggc gcagtttcga
    15841 ggcgatgggc gtttcactgg tagtgcatcc gcataacccg tatgttccca ccagccacgc
    15901 gaatgtgcgt ttttttattg ccgaaaaacc gggtgcagag cccgtctggt ggtttggcgg
    15961 cggcttcgat ttaacccctt tctatggttt tgaagaagac gccattcact ggcaccgcac
    16021 cgcccgtgac ctgtgcctgc catttggtga agacgtttat ccccgttaca aaaagtggtg
    16081 cgacgattac ttctacctca aacatcgcaa cgaacagcgc ggtattggcg ggctgttctt
    16141 tgatgatctg aacacgccag atttcgacca ctgttttgcc tttatgcagg cggtaggcaa
    16201 aggctacacc gacgcttatt taccaattgt agagcgacgt aaagcgatgg cctacggcga
    16261 gcgcgagcgc aattttcagc tctaccgtcg cggtcgttat gtcgagttca atctggtctg
    16321 ggatcgcggc actctgtttg gcctgcaaac tggcgggcgc accgagtcga tcctgatgtc
    16381 aatgccgcca ctggtacgct gggagtatga ttatcagcca aaagatggca gcccagaagc
    16441 ggcgttaagt gagtttatta aggtcaggga ttgggtgtaa ctccctcacc cccattcccg
    16501 catccgctga tgcaacgtca acgacggctt ctcggcaaac agctgctggt aatccgtggc
    16561 aaattgcccc agatgccaga atccccactg catggcggcg tcttttaccg ttgtgctttg
    16621 cgaccacggg cttatcagtt cgcggcgtac ggcgttcagg cgaatgcgtt tcagccacgc
    16681 gtttgggcca atgcctaaaa tagcgtgaaa cgcgttttgt agcgtgcggc gactgacatg
    16741 cagttgatta cacaagtcca gcaccgtcac cagctcagac atattttcca gcacatattc
    16801 acgggcgcgg gaaagtaatc tacggtaact ctgatgactg atgctttccg ccgtcaccat
    16861 tggttgcgcg tcttccagca tcgcccccat cgccattagc aaattatccc ccagcacttt
    16921 tcgcactgca ggctgatgga gattttccgg gttctcgcaa aacgtcgcca gcgcctgttg
    16981 gacaaagccc cacagcgcgg ctttatgctg ctctttcact tccagcgccg actggctacg
    17041 cagcatatgt aatacccgat ccggattgtg caaaaagtta gcctgacggg tgatgacatc
    17101 ttcagacagc actacgccca ggatcgtata atcgtccggc gtacttaatt caaactcggt
    17161 tccaccgggg cgcgtcgcta tttctgcgct tcccagacat tgcgaaccga taaatccctg
    17221 ctcaccgcgc gtcgccggaa tgccaaacca gaacgagttc ggccagacca ggcacgactg
    17281 acgcagcgcc agaccggtgt attcacgaaa aacctgaata tcatcgagca gaatttccgt
    17341 aaattcacca tgaaacttgc ccggatgcag ctgatcgtaa atctgctgcc aggcggtaat
    17401 cgtcaaagca tgttcataga catccgttgt ctgtcgttga tgaacattat ccacttcgac
    17461 cttcggcgtg agcttcaggt tttcgggtaa gggttcatga taaagatggt gcaaattggc
    17521 tgtacgggtc tttttcatga tgttaatgcc gggtgttgta ggacacccgg cacctccgac
    17581 aggttaatgg ggcttgagac gataacgact actgcgttta cgtaacgtcc cggcagaaaa
    17641 gagctgttcg agcgcatttc tggctttttc cagcggccag ccaaagtggg cagccacttc
    17701 tcctgccgtc attccctgac gtactgatgt taacagcgcc aacaactcgt cagcagattc
    17761 cgcgacgata actggcgcgt cgggttcctt tacgggttgc ttcggctggc ggttaaagcc
    17821 agtaaccagc cactgggtgt catcgtctgg ccgaccgatc tccttgcggc tgatgacacg
    17881 cccggtacgc atcgcggcag cacaacctgc gtccagcgcc gcacgacacg ccgccagatc
    17941 gccttccacc accagcgtta agcgaccagg gtcaagcact tcgtgactga gcagacgaac
    18001 gttagctgct ttgagcatgg catccgctgc atctatcgcg gcgaccatgc cgtccacttc
    18061 cagcaatccc agtgcattga tcatcggcaa cctccgttac gcacgctgga ttggattacg
    18121 cgcgatttcc agcactgcat cggtaaaggc gttacaggct gctttacacg cggcctggct
    18181 accggttaaa aatgctgccg agtagttggt ttcagacggt ggcgggacat aggtcaccag
    18241 ctgtacgtcg gcagatttca acgctgcatc aatgccgtag gtcgcttcca gcggcggtgc
    18301 caccagatac gccatcggat cgccaagcgt gatcccggcg gttgatgaaa gataagaacc
    18361 ggtacgcgaa actacatgtg ccaggaatgc ggtgttttct gcgtcattag cccactggaa
    18421 agcagcgcca ttttcaatat gcgcaaccat cgcatccaga ccagcacgca cttccgccgg
    18481 gtttggccca ccaagcataa tcagcacttc accggcagtc ggtgacggtc cgtgtgctgc
    18541 tcccgcatac agcgagcggc catataccac ttcgaccatc gcctgcttgg tcgcttcgtc
    18601 agccgcaatg taggtgacgt catcggaatc agcagaaatc agcccgaggc tacgaatatg
    18661 cggcggcaat tttagttcac gcgcaaattc agcgttaacg gaggcaatca cccgcatggc
    18721 ggttaccgac ggtcgaatca aatctaaagc tggcatgatg cctccttatc gggtcatgtt
    18781 gatgccggat gctttctgct ccagcatacg tttcgccaaa tccacaatga cggctgcggc
    18841 ttcaaccggc ggcgtgccgc cctggtgaat gttagagatg caggtgcgat cggcctcaac
    18901 ggtggtcgcc atacgcggcg agtaaacggc gtaacaggag aggctttctg actgacccaa
    18961 ccccggacgt tcccccacca gcaggatcac cactttcgcg ccgaggatct caccgatctg
    19021 atcttcaatc ttcacgcgac cataacgaac aaagaatggc gtgccgactt tcagcccggc
    19081 ctgtttcagg cccgccatca gcggcggcag gatctcttcg tagttcacgg tgatggcatc
    19141 agttgacagg ccatcagaaa taacgacctg tacgtccgga ttagcaacac actgtgcttt
    19201 cagcgcttca acagcttctg cacacaggcg gcggcccata tccgggcgag tcaggtagag
    19261 atttttgtcg ctaatttcgg agcgtacttc cagcaagccc tgcgctttca cccactcttc
    19321 cggcacttct ttcagtacgg tatctttcga acgagagtga tcggccagga aacgcagcag
    19381 cgcctgcgta cgtggacgcg gaccggtacg accggtacaa acgcgggcca cggtgctgcg
    19441 gcgcagttct gttaatacgt ctgcgcgatg cgggttttca acaccaatcc acgcttttgc
    19501 ttccgcagaa cctaaatcca gcgcgcagct ttccgaggtc accggtgccg cacagttagt
    19561 ggtggcgcac tttgcttctg acggcgcggg ggccgcttgt cccattgacg ccatcacgct
    19621 gcgtacaatt tcttcaatct gtttttgatc catgatgtgt tatctccgcg tcatcagaag
    19681 aacagtgacg gatcgcccgc ccgtttggtc aggcgaccgt ttgccataat gcccatgctt
    19741 tccagccagc gttcaaactc cggtgacggg cgcaggttga gtaactgacg cacagtggca
    19801 gtgtcgtgga atgcggtggt ctgatagttg agcatgatgt catcgcccag cggcatcccc
    19861 atgatgtagt tgcagcctgc ggtggcgagc aggatcatca ggttttcgtt gaggttctgg
    19921 tcagcgtcag cgtggttggt gtaacagcag tcacagccca tagagatgcc gctcagcttg
    19981 cccataaagt gatcttctaa gccagcacgg ataatctggc ggtcgttgta gagatactcc
    20041 ggcccaataa agccgaccac ggtgttgacg ataaacggat cgtaatgacg cgccagccca
    20101 tagttacgtg cttccatcgt cacctggtct gcgccgaagt tagcgccagc agacagcgcc
    20161 gaaccttgcc cggtttcgaa gtagaggcag ttttccccgg cgatacggtt gaactcagca
    20221 cccactgcgc gcgcttcgtc gagcatcgcc agttccacgc caaactcttt cagccctttt
    20281 tcgctgccac agatactctg gaaaatcagt ccgccaggcg cgccgcgacg aatcgcttcg
    20341 atctgggtgg tgacgtgcgc cagtacgcag ccctgagttg ggatgttgaa tttgtcgatc
    20401 acgccgtaaa tggtgtccaa cacgcggctt aagttttcca cgtcgtcagt taccgggtta
    20461 acgccgatca ccgcatcgcc caccccgaag gaaagccctt cgtagatttg tgcagcgata
    20521 ctttgcacgt cgtcacgggt gtcatttggc tgcaaacggg cgctgaaggt gcccggaata
    20581 ccgatggtgg tattggcctt tttgattacc ggcattttct tcgcgccgta gatcaggtcc
    20641 gcgttggagc aaatcttcgc taccgccgcg accacttccg aggtcagccc tttgcgggta
    20701 aaggcaatgt cgtccacgct ggtttcatcg ctcagcacat actcacgcag ttcgctgatg
    20761 ctcctgtttt taatctggtt gtaggccgtt tcgttaacat cgtcctgaat cagccgcgtc
    20821 acgcagtcat cttcataggc aatcaccgga ttattgcgga tgtccgctac ggtcatttcc
    20881 gacaacacct gctttgccgc cacgcgctcc tgtgagcttg ccgctgcaac gcccgccagc
    20941 acatcccccg aacgcagttc gttggcttta gccagcacct cttttacatc cttaaactga
    21001 tatacattgc cgaacaatgt ggtctttagt ttcataagtc gttccctcag gaaggaaatg
    21061 cgagtgattt caccgtcacc ggcacaaccg atccgccaaa aagaggcgta ccaatgtcga
    21121 tatagtcccc cgcgcggaca atcacttcgt caatgactgc caacgggagt tgttgtagct
    21181 gtgggcgcaa caacatgccc agagctttac caaagtcctg cccggccacc accagcaggg
    21241 gatgcggatt tggaaaacgc gcgacgaaat cgaccagcgc gttgatgacc gtcagtaccg
    21301 cggcgtaacg cacaggcagc gaggcgggaa gcgccagcac gtacgcgtca gttttgggat
    21361 caagatccag ctgaagcagc gcctgttgcc aggcactcac cagatccgtt tcatcaatcg
    21421 ggatcgccac cggcaaattg cgcagcggca gttgtacgcc ctcaagccag attgtgctgc
    21481 cagagagcga aagggtatgc gcgcccgcgc caatcaccgt ggcgcgcacg gtttgcgccg
    21541 gaaactgtac gttcatctca cgcaggcgcg gatggtcatg cagcgccgtt gccagcagcg
    21601 ggccaatatc ggcaaaacag aacgggtcgg cgggctggtg gcgataacat tcgcccacgc
    21661 cgccagaaag cgtaatgatt tcgggcgtaa cacctgcggg cagcaaaccg gtttgcatca
    21721 atgcctgcgc gagcggcgag agcgttccgt caatcacttc gacaatcagt tctgccatcc
    21781 gccgggtaac ctgcaccagc tgcgcgccgg tcagcgaacg ggcgtcggtg cctgcgccga
    21841 agcactcatc cacaatcatc tgccccggtt tatgagcgta aaccacgcgc ccctggctgt
    21901 cggtttccag caggcgacca ccgacgttga ggcaggcagt gccgctgatt tttccggcat
    21961 cgaacagggc gtagttcgca gtgccaccgc cgatgtcaat attcagtacc cgacacagcc
    22021 gttgttcaga aagggtttgt gccccggcac cgtgaccggc aatgacggat tcgaggtgcg
    22081 gcccggcgct ggcaacgaca aaatcgccca gcgactggga gagcaccatc accgccggac
    22141 gagcattgcg ggttttcgcg ctttcaccgg tgatgatgat ggcaccagaa tcaacgcttt
    22201 ccggcgcaat gcccgcagcc tgatattgct cgaggattaa ggttttcagt tccgcttctt
    22261 ttaaaccacc ctgtttatcg acaggggtaa agaacaccgg gctttgccag ctaatttcgc
    22321 gtttaatgaa ttcgtagcgc ggcacctgcg acaccgccgc acggttaacc agctccaggt
    22381 gggagaaaat cacctgggtg gtggtggtgc cgatatcgat accgacgctc aatagctggc
    22441 gagtgttcac gattgtgcct ccgcttcggt tttagccgtg gtcgcgtctt cttttggcac
    22501 cagcatcatc gccacgccaa tcgccgttac gccgccgatc aacttgccga caatcatcgg
    22561 gaagatcatg gcgttcatgt tggcagcggc gaagcctaag tggtcgccca gggcgaaagc
    22621 agcggaaacg gcgaaggcgc agttgatgac tttgccgcgg gtatccatct gcttcatcat
    22681 gccgaacatc gggatgttgt tggcaagcgt tgccaccatg ccggctgccg cgatgttgtt
    22741 catattcagc actttaccga cgctcatcag cggtttttca aaccagcggg tcagcagcag
    22801 caccattgga tacgccccta acagaacgca ggagatagaa ccgataactt caatggcgcg
    22861 catcacttca ccgggtttat cgccaggggc cataaagata gggtccagac cggggatcag
    22921 ttcccagcca agcagaaact taactaccgc ggcagcaaga ccgagggtga tcaatgcaac
    22981 gaggaatttg gcgaagatct ggaagccgtt gatcattttt tccgggatga atttcagccc
    23041 cagcgccacc agaatcgcaa caatgatcac cgggatcatg ttcatcagga tcagggcgaa
    23101 agtgaattcc accggctgac cgttgatctg cacaccggag tacatagcaa ccagaccacc
    23161 agcaatacag ccaatcggaa tggtcacaat gcccgccagc acgccgagcg ccagataacg
    23221 acggtcagaa ggttcgataa tgccgagcgc caccggaatg gaaaacacaa tcgttggccc
    23281 catcatcgac ccaagaatta acccagagta tagccacgct gctacgtcgc cgcccgccag
    23341 ctctttggcg aggaagaagc cgcccatatc gcacgccagc agcgttccgg cgaacattga
    23401 cgggtttgcg ccgagcattt cgtaaaccgg gataattacc ggcccgagta cgtgagccag
    23461 taccggtgcc agcgcggtca taccgaccat cgccagaccc agagcgccca ttgccataaa
    23521 gccttcttcg aactgaccgc ctgatccttc gatactttta ccgaacttac cgaggaaacg
    23581 agcagaaccg ccgaactgcg acaggatcct gtctacggca gctatcagca taaagaacat
    23641 catgatgtac atgatgattt cgttaattcc catcgccttt actccctgtt agttgttatt
    23701 tattggcgga tgcggcgtaa acgccttatc cgccctacat gtgcaatccc gaaggccgga
    23761 tcagacgcgg caagcgtcgc atccggcaat tgcaccgcgc cactggcgga tgcggcgtga
    23821 acgccttatc cgccctacat gtgcaatccc gtaggccgga taagacgcgg caagcatcgc
    23881 atccggcaat tgcaccgcgc cactggcgga tgcggcgtga acgccttatc cgccctacat
    23941 gtgcaatccc gtaggctgga taagacgcgg caagcgtcgc atccggcatt tattgcgccg
    24001 ccgcgtacag tccgacaatc tgttccaggc tggcggtacg cgggttactg cgcagacaaa
    24061 tatcttccag cgcggcctgc gcccatgcgc cgtaatgcgc agatgtcgca ccaacatcgc
    24121 ccagtcgttt accaatccca acttccgcaa tcagctcact taccgcgtta atagcgtcac
    24181 gatcgtcgga ttttttagtt cgcagtgccc gaccaatctg actaaagcgt tcacgacaaa
    24241 ccatccggtt aaattccatc accgttggca gcaacatggc gttcgccaga ccgtgtggaa
    24301 tatgcagcgc cgcgcccggc tgatgcgcca tcgcgtggca caacccaaga cccgcactgg
    24361 aaaacgccat tcccgccata catgaagcca gcaacatgct ctcgcgcgcg gcaaggtcgt
    24421 gaccgtagcc caccgctttc ggcagcgatt tgccaatcat cgcaatggca ccaatcgcca
    24481 gactgtcggt aaacggtgta gcgttcaggg cgctgtatgc ttcaatggca tgggttaacg
    24541 catcaatgcc ggtcatcgcc gtgacatgcg acggcacacc ttcggtcaac gcggcgtcga
    24601 gaatcgccac atccggcatc agcgaggcat gggctaacac ctgcttgcgc ccgctcaccg
    24661 cgtcgataat caccgttaca ttggtggttt cagagccggt tccggcggtc gttggaatgg
    24721 caatcagcgg caagcgcggt tgcagaacgc tggtttctga catctctgcc agcgtgctat
    24781 ccgggttcgt caccagcaac gccacggctt tcgccgcatc cagcaccgag ccgccgccaa
    24841 atgcgatcac cccatcacag cctgactcac gcaactgcgc cacggctgca cacacgtcgg
    24901 tgatgcacgg ttcgcccacc ggacatggcc agagcgtcat ggcgatgcct ttaacagcca
    24961 ggctgcgcgt cagcccggcg gtcatccctg cctgatgcaa aaagctgtct gccatcacga
    25021 acagatgttt cagcccacgc gtttgcgctt gctgtccgca actgctcacc gagcccggac
    25081 cgcaaagcgt caccggtgga acgctaaatg tttttacccg ttgcagattc agggtatcga
    25141 acgcctgaaa gagcgcggtc tgcaattcat tttgcatata gcccctccgc tttctctcgc
    25201 ccactactgg cgatcgccag cggggtcata aacaaactgt gctgcggtaa atgcacctgt
    25261 aacgccggga attgtttgcg aaacagttcc gccacgcccg gttgcataca agagccgccc
    25321 gccagccata aatcagtaat gccctgccct tcaatatgcc gggcgacaat gtccgccatt
    25381 ttttcgtaca ccggtttcac cgtaggccaa atctcttcac cgtgaccgcg cttgtactgc
    25441 tccgcctctt ccagcgaaat acggcgattt ccggcgagag tcagagagat gtgatgtccg
    25501 ccggttgctt catccgccga gtacgtcacc ttgccctttt tcacgatggc aatgccggta
    25561 gtgccgccgc cgatatccac tacaccggcg ttgtccagtt gcagcagatc cgcaactgcg
    25621 gttggctcat ccagcacatg gctcacttcc agtccagcag actccagcac gttgatggaa
    25681 atacgcgggt ccgtacctgg aggaaatgaa gtcgccgcat ggctaaaacg gcgaccgaat
    25741 tgctgctcga gcgtgtcgag atggcgacga acaatggtga cagcgccgaa gaaatcccag
    25801 acgatgccgt cgcggaccac atcggcccag tcgaggcata ccgccaccga ctgaccgtcg
    25861 cggtcgacaa ccatcgacac cacatcgcag gtgcccagat ccacccccag ccacagcgga
    25921 gattccgtcg cggcgggcgt ctggttacac agcgttgccg ccgtttgcag acgtggggtg
    25981 agccattgtt cgtcgtgcgc catctgttac tccttaaaca atgcgaaacg catcgactaa
    26041 tacacagcga cgcagacgga caaacgtacg cacgctagtt accccttcac cggttggcgt
    26101 ggtgatggtc atggtggtcc agccttcccc gcccagcccc agcccggcaa tgcgcggtcc
    26161 gttcttaacg aaaatgctgg tatcaatagc attcgccatc tggttcatgt tttcgatgtt
    26221 gcgcgagtgc attgccgccg tgtggtggca accgccttcc agtttcaccg ccagcgcaat
    26281 ggcatccgcc acgttggcga cgcgcacgac gggcagcacc ggcatcatca gttcggtcac
    26341 ggcaaacgga tgttctgcag tggtttccac aaacagcaaa cgcgtttctt gcggcacgtt
    26401 caggccgatt gccgccgcga ttttcgccgc atcgcgacca acccagtcac ggctgacggt
    26461 gccttttccg cgctcgtcga tatttttcag caacaccggt tgcagctgct gcgcctgttc
    26521 tgcggtcagt ttcaccgcgt gctggccttc catcagacgc atcagttcat cggctacgct
    26581 atcaacaaca atcagtacct tttcgtcggc acaaatgatg ttgttatcga aagaagcgcc
    26641 tttgacgatg gactgagcgg cacgggcgag gtcggcggtt tcatccacca ctaccggcgg
    26701 gttgccagcg cctgcggcaa tcagacgttt attggtgtgt ttacgcgccg cttctactac
    26761 cgcttcgccg ccggtcacca ccagaaggcc gatgcccgga aacttgaaca aacgctgcgc
    26821 ggtttcgata tccggatttg ccacagtaac cagtaagttt tccggcccgc ctgcggcaac
    26881 aatcgcctgg ttgagcagcg tgatcgcccg ctgggagact tttttcgccg ccggatgcgg
    26941 ggcaaaaatg acgctgttgc ccgcggcaat caggctgatg gcgttattaa ttacggttgc
    27001 cgccgggtta gtggacggag ttaccgaagc caccacgccc caaggcgcgt tttcaattaa
    27061 agtcaggccg ttgtcgccgg tcagcacctg cggagagaga cactcaacgc ctggcgtgcc
    27121 gcgcgcctga gcgacgtttt tggcaaattt atcttcaacg cggcccatgc cggtttcact
    27181 gacggcaagt tccgctaaat ctctggcgtg tttttcgcct gcctcacgaa tggcagcaat
    27241 ggctaactgg cgcattgcca cgctttttaa cccttgctgg gcgactttgg ctgccgcaac
    27301 ggcgtcatcc agggacgcga aaacgcccat ctcatgaacg gcggcgggcg gcgtgtcact
    27361 gctttgcatt ttcagcagta ccgctttcac cacctgttca atatcctgtt gattcatgat
    27421 gttctgcctt atttgtggaa aattacctga ccgccagaca ccacctcatc gacaatgcca
    27481 atcacgcaca gatcgaccgg tgacgtttcg cttttatgcg cctggcgggc ggaactgcca
    27541 ctcaccagta acacccactc cccggttccc gcgccaatat tgtcgatggc gacggcgcat
    27601 tgcccgtcag gattaccttg tggatcaatc atttccacca tcagcaattt gtcatgcgcc
    27661 agtccgtgat ggcgtacggt acaaacaatt tgtccagtga cgactgccag tttcataccc
    27721 gcctccgtgg cgtatttcag gtaaaagctc cccctaccct ccgcagaagg taaaatgaaa
    27781 aaggagagag cgtgacgccc gaatcgacgt cacacagggt gattacaggt tgctgctatc
    27841 gcctttcagg ccgatcggga agacttcttc caggtcgccg tgcggacgcg ggataacgtg
    27901 tacagatacc agctcgccga tacgctgtgc cgctgcggca cctgcatcgg tcgcggcttt
    27961 acaggctgca acatcaccac gaaccatggc agtacacagg ccgccgccaa tctgcttaac
    28021 accaaccagc ttgacgcgcg cagctttaac catcgcgtca gaagcctcaa tcagtgcaac
    28081 caggccccgg gtttcgatca ttcctaatgc ttccattgtg ctttcctctt tatcagggtc
    28141 cagaacggga ccgttcattc aaccagtgtt tgtaaactgc tttcgcggtt cacttctgtc
    28201 tgacgcggca cagctgccac cagcgccagc tcgataattt cctgcacgct acaaccacga
    28261 gagagatcgt gcatcggcgc ggcaagtcct tgtatcaatg gcccgacggc acgatatccg
    28321 ccgagtcgtt gtgcgatttt gtagccaata tttccggctt ccagcgacgg aaaaaccatc
    28381 acattggctt tgccctgtag cgggctggca ggcgcttttt gcgccgccac ttccggcacg
    28441 aaggcggcgt caaactgcaa ctcgccatca accaccagct ttggtgcgcg ctcacggacg
    28501 atttctgtcg cctgctggac gttggcaacg caggggtgac gggcgctacc gttactggaa
    28561 aacgacagca tcgccacgcg cggctcttct ccggtgaggg cgcgccaggt ttcggcactg
    28621 gcaagcgcga tatccgccag ttgcgccgcc gtcggctgtg gcaccacgct gcaatcggca
    28681 aagcccaacg ccggaccgct gtactgtggc agcatcagga aaatggatga gagcgtttta
    28741 cagcctggct gcaagccgat aatacgtaat ccggcacgca gcacattcgc cgtggaagag
    28801 aggttgcccg cgatacagac atccgcttta ccggcgctga ccattgctgc ggcgaacatc
    28861 agcggatcgg taagtttttc cagcgcatcc ggcggcgttt tttcgcccgc gcgggccagc
    28921 cagcgatgag caaattcttc ccgcattgcg aggttgccat gcggatcaat cacctgtagc
    28981 ccgtccatcg ccacgccgtg actgagcgca aactgacgaa gttcaaacgg attagcgacc
    29041 agaatgggcg ttgccagacc ttgttgatgt aaatattgcg cagctttcag cacacgttga
    29101 tctaacgcat ccggaaaaac cactctggcg ggcgctcgca acgccagttc acgacaacgt
    29161 tcaataatca ttgcgtttcc cccagtcgtt gttggatctg cttcaccgtt agcggcggct
    29221 tcgttacgct caggatcatc attacgtaga ccgtgctcga aaggcggttc agcgcctgca
    29281 agatgtccgg gcgtaatact tcaaagctgc gggtgataaa cacctgcgcc gctacggttt
    29341 cggtttcccg cactttggta cgtagcagat tgagcaacgc cgcatcgcgc ccgtggctgg
    29401 cttccggcac cagatgatcg tggtcgagat agcgcagcgg ctggtgagaa agtcgatgca
    29461 gatcttcgtc agaaagcccg acaatcgcct ggcagcccag cggttccccc agcgcatcgg
    29521 cgcgcataat gttgccaaga cgcgaacgga tatccgccag ccacggctgc cacggttccg
    29581 ccagttcaat ttgcagccac actgccagcg caatggtgct gtcgagaacc gcgcgaaaac
    29641 ccagacgcgg atcgcttttg gcgaccattt tttccgccga caggtgagtc agcgtatccg
    29701 gcttttttgc caccggctgg cggcacagtt cgcagcacgc ctgcggatgt tcatcgctac
    29761 tggtcagccc atgaacgggc tgcggctgct gctgttcatc gtcaacaaac aggcggccct
    29821 gctcgtcaat aaacttgatg cgcagatggc ggctttccag caactcccgg gcagagggcg
    29881 tcaggcgact gtccgcaggg agatggatct ctgcgccttc gctgagcgta tggttcgctc
    29941 ttagccatgc ttcggtgatg aaatctttca tagggattgc cagttagccg gccaggcgac
    30001 atacaggaat ttcacgctgg atgtcgtacc aaattcgatg ctggagcctt tcgggataaa
    30061 catcacgtcg cctgctttgg cgatcatggt ttcgccttcg tggcgaacat ggagctcccc
    30121 ttccagcacc atgtcgattt cgtcgtagtt cagggtccac gggaagaatg cgttctccca
    30181 ctgcataaaa ccggcggcca tgctgctgcc gtcgtctccg gtcacaagat cagtcaagcc
    30241 cacgcaatgc ggttgtgcgc catcaaaacg accaaacttg acgctgctgc catcaatcac
    30301 tttgatgccg cctttgccgg tcaccgattt gaagctcggc tgcattgcgc cctgctccag
    30361 cgactgtttt tccttcatca ctttttccat cagctgtgcg accaggcttt cggtaaactg
    30421 gccttccggc agctgggcaa tgatggtttc gcggatgcgc tggctttcgg ttttgtcggc
    30481 tggcacgctg gcgggcacgg acgccgttac cgggatcgat tcatcacatt cggtgatggt
    30541 aaaacccagt agatccgcca ctttacgggc ttccggggta ataatgctgg cgcgcagaac
    30601 cacggacatt gcctgttcgc cgcgcgcgtg tgcttcacga atatcattcg ctgtgatgag
    30661 ttttttcacc tgcttcctcc tgtttggtga gagatgccag ataatccacc agttgctgca
    30721 cgctttgcgg gtcgtggctg tttagctcga aaatcggctc ttcgaaaccc gtctcaagca
    30781 ataactttcg cgttgcggcg acgtcagcat ctggcatgtc cgttttactg atgacggcga
    30841 tttgccgttt gctgacgcca atatccagca acccggcagg caaccgactt tccgggtcat
    30901 ttgcgccatg gacatagatc agcatgtcta catcctgtag cgtggtaatt aaggcgtgat
    30961 accagcgggg atggctaaaa tattcacccg gcgtatcaat atcgccctta tcattaaatt
    31021 ccacggcctg tgtttttctg gcgagggtat aattcccctg caacgcatta aataacgttg
    31081 tttttcctgc cccgacgcta cccacaaacg caattcgttt catgaatgcc tcgtattaac
    31141 ttttggtcat ttcacaaagc gtataattta ataagcgacc taaaccgctg acggtttgtg
    31201 ataacgcttc ttctactgcg cccaccgagc cataaatcac caatgcaccg ctgaagcggt
    31261 cgagaaaacc gatatgcaca tcagcggctt tcagcgctaa atcaccgacg atcatcgccg
    31321 tttcgccggg agttagcgtc ataatgccaa ttgcgcccgc atccggaacg ccgattttct
    31381 tcgccagttc ctcaccggga tgcgcgatga gatgcgccag tgtgacctgt ttgccgggca
    31441 caaattcctg aatgatgcgt tctttatcca tgagtcgccg cctgtgaaaa actttcgtgt
    31501 atcgtgacaa tttttcgtca gagtaaataa caaaattcgg caagcgcgtt taaaaggtga
    31561 ggtagatcac taagaatgga gagatattcg ctgtggtgca taaactttca tatgcaacgc
    31621 atgaatataa ataaaataat atcgagatat tatatagact gctttttttt attattcgca
    31681 aactcagtaa taagctttac taatatattg cagccaaacc gcttaccctg aatattcagg
    31741 gtaagcgtga gagttaaaaa agaattacag cggttgggtt tgcgcttcaa ccacggccag
    31801 cgccaccatg ttgacgatac gacgcaccga tgcgatcggc gttaacacgt gaaccggttt
    31861 cgccacgccc atcagcaccg gaccgacagt cacaccttcc gagctggaaa cgcgcagtaa
    31921 gttgtaacta atgcgggcag cttccatgtt cggcatcacc agaatattgg cagaaccttt
    31981 caaagggctg tccggcatac ggtcgttgcg aatcgcttcc accagcgccg catcgccgtg
    32041 catttcacca tcaatcatca gttctggcgc acgttccctg accagttcca gcgcctgacg
    32101 cattttgctc gacgacgggc agtcagaaga accaaagttg gagtgcgaca acagagcaac
    32161 gcgcggttca ataccgaaac gacggacagt ttctgccgcc atcaaggtga tctccgccag
    32221 ctcttctgcg tctggttcgt cattgacgta ggtatcggca ataaaggtgt taccactcgg
    32281 cagcagcagc gcgttcattg cacctgcggt gtgaacgcca tcgcgataac caaagacatt
    32341 tttcaccacg ctaaaatgtt catgataatc acccaccgta ccgcaaatca ttgcatcggc
    32401 ttcgccacgc tgaaccatga tcgcgccgat cactgtcggg ttactgatca gcgcccgctg
    32461 cgcctgttcc tgagtgacgc cgcgacgctt catgatctgg aagtattcgg tccagtactc
    32521 tttaaagcgc ggatcggatt cgttattgac gatctcaaaa tcaacgcccg ctttgatctg
    32581 caagcccagt ttctgaatgc gcatttcgat cacgttcgga cgaccgataa ggatcggttt
    32641 cgccagtccc agcgttacca gttcctgagt ggcatgcaga acgcgcgcct cttccccttc
    32701 cggcagaact acgcgcttcg gcgctttgcg agcctgggag aaaatcggct tcataaacag
    32761 gttggttttg taaacgaact cagtcagctt gtcgatgtag acgtcgaaat cagcaatcgg
    32821 acgcgtcgcc acgcccgact ccatcgccgc tttcgcgacc gcaggagcga tcttaacgat
    32881 caagcgcgga tcaaacggtt ttggaatgat gtattccgga ccaaagctca gatcctgatc
    32941 gccatacgct gaagccacca cttcgctctg ttccgcatgg gccagttctg caatcgcgcg
    33001 taccgccgcc agtttcatct cttcgttgat ggcggttgcg ccaacgtcca gcgcgccacg
    33061 gaagatgaac gggaagcaaa ggacgttgtt gacctggttt ggatagtcag aacgaccggt
    33121 gcagatgatg gcatccggac gcacttcttt cgccagcggc ggcagaattt ccggttccgg
    33181 attcgccagc gccagaatca ttggtgcccg agccattttc ttcaccattt cctgggtcag
    33241 cactttcggg ccggaacagc ccaggaaaat atccgcacct tcaatcacat catcgagggt
    33301 acgtttgccg tcatccacca ccgcatatgc ggctttggtt tccgccatgt ttggctcacg
    33361 gccttgatag ataacgcctt ttgaatcgca aaccacgatg ttatgttttt gcagacccag
    33421 cgctaccagc agattcatac aggcgattgc tgcggcaccc gcgccggaaa ccaccatccg
    33481 cacgtcggag atgtttttct ccaccacgcg caagccgttg aggatggcgg cagtgctgat
    33541 gattgccgtg ccgtgctgat cgtcgtggaa taccggaata ttcatccgct cgcgcagttt
    33601 ctgttcaata tagaaacatt ctggcgcttt aatgtcttcg aggttgattc cgccgaaggt
    33661 cggttcgagc gcagcgacaa cttcaataaa tttgtccggg tcgagttcat caacttcaat
    33721 gtcaaataca tcaatcccgg cgaatttctt aaacagaacg cccttgcctt ccatcaccgg
    33781 tttgccagcc agcgcgccaa tgttgcctaa ccccagcacc gccgtaccgt tagagatcac
    33841 cgccaccagg ttaccgcggg cggtatattt gtaggctttt aacgggtctt tttcgatttc
    33901 aagacaaggt gcggcaacgc cgggtgagta ggccagcgcc agatcgcgct gcgttgccag
    33961 aggcttggtt ggagaaacct ggattttccc tggaactgga aattcatgga aatcaagtgc
    34021 actttgtttt aactggtcat ccatttggtt gttcctttca cgtaacgttc acaaataaag
    34081 tgtgtgggca acaccccctg cccacaacgt ggcgcacatt attaccttgc cggagtctac
    34141 agactttgag caagtccaaa ctctcaccat taatataatg ttttggtaat aatcctataa
    34201 cactgatgtt acctgcttaa tccagcaata ccatgcctgt ctgctatgct tttttgatgc
    34261 gtttagcgaa atttctcaga agtgtgaatt aacgcactca tctaacactt tacttttcaa
    34321 ggagtatttc ctatgaacga gttagacggc atcaaacagt tcaccactgt cgtggcagac
    34381 agcggcgata ttgagtccat tcgccattat catccccagg atgccaccac caatccttcg
    34441 ctgttactca aggctgccgg attatcacaa tatgagcatt taatagacga tgctatcgcc
    34501 tggggtaaaa aaaatggcaa gacccaggaa caacaggtgg tcgcagcgtg tgacaaactg
    34561 gcggtcaatt tcggtgctga aatcctcaaa atcgtacccg gtcgcgtgtc aacagaagtt
    34621 gatgcacgcc tctcttttga taaagaaaag agtattgaga aggcgcgcca tctggtggac
    34681 ttgtatcagc aacaaggcgt tgagaaatca cgcattctga tcaagctggc ttcgacctgg
    34741 gaaggaattc gcgcggcaga agagctggaa aaagaaggta ttaactgcaa cctgacgctg
    34801 ctgttttctt ttgcacaggc acgggcctgt gcggaagcag gcgtttttct gatttcgccg
    34861 tttgtcgggc gtatttatga ctggtatcag gcacgcaagc cgatggaccc gtatgtggtg
    34921 gaagaagatc cgggcgttaa atcggtgcgc aatatctacg actactataa gcaacaccac
    34981 tatgaaacca ttgtgatggg cgcgagcttc cgtcgcaccg aacaaatcct cgccttaacc
    35041 ggctgcgatc gactgactat cgcaccgaat ttgctgaagg agctgcagga aaaagtttcg
    35101 ccagtggtac gtaaattaat cccaccttct cagacgttcc cacgcccagc tcccatgagc
    35161 gaagcggagt tccgttggga gcacaatcag tatgcgatgg cggtagaaaa actgtctgaa
    35221 ggcattcgtc tgttcgccgt tgatcaacgc aaactggaag atcttcttgc cgccaaacta
    35281 taaaccagcc acggagtgtt atatgtcccg aaaagacctt gccaatgcga ttcgcgcact
    35341 cagtatggat gcggtacaaa aagccaattc tggtcatccc ggcgcgccga tgggcatggc
    35401 tgatattgcc gaagtgctgt ggaacgattt tcttaaacat aaccctaccg acccaacctg
    35461 gtatgatcgc gaccgcttta ttctttccaa cggtcacgcg tcgatgctgc tctacagttt
    35521 gctgcatctg accggttacg acctgccgct ggaagagctg aagaactttc gtcaactgca
    35581 ttcgaaaacc ccaggccacc cggagattgg ttatacgcca ggcgttgaaa ccaccaccgg
    35641 tccacttgga caaggtttgg cgaacgccgt cgggctggcg atagcagagc gtacactggc
    35701 ggcgcagttt aaccagccag accatgagat cgtcgatcac ttcacctatg tgtttatggg
    35761 cgacggctgc ctgatggaag gtatttccca cgaagtctgt tcgctggcag gcacgctggg
    35821 actgggcaag ctgattggtt tttacgatca caacggtatt tccatcgacg gtgaaacaga
    35881 aggctggttt accgacgata cggcaaaacg ttttgaagcc tatcactggc atgtgatcca
    35941 tgaaatcgac ggtcacgatc cgcaggcggt gaaggaagcg atccttgaag cgcaaagcgt
    36001 gaaagataag ccgtcgctga ttatctgtcg tacggtgatt ggctttggat cgccgaataa
    36061 agcaggtaag gaagaggcgc acggcgcacc gctgggggaa gaagaagtgg cgctggcacg
    36121 gcaaaaactg ggctggcacc atccgccatt tgagatccct aaagatattt atcacgcctg
    36181 ggatgcccgc gaaaaaggcg aaaaagcgca gcagaggtgg aatgagaagt ttgccgccta
    36241 taaaaaggct catccgcaac tggcagaaga gtttacccgt cggatgagcg gtggtttacc
    36301 gaaggactgg gagaaaacga ctcagaagta tatcaatgag ttgcaggcga atccggcgaa
    36361 aatcgccacc cgtaaggctt cgcaaaatac gcttaacgct tatggcccct tgctaccgga
    36421 gctgctcggc ggttcggcgg atctggctcc cagcaacctg accatctgga aaggttctgt
    36481 ttcgctgaag gaagatccgg cgggcaacta cattcactac ggggtgcgtg aatttggcat
    36541 gaccgctatc gccaacggca tcgcgcacca cggcggcttt gtgccgtata ccgcgacgtt
    36601 cctgatgttt gttgaatacg cccgtaacgc cgcgcggatg gcggcactga tgaaagcgcg
    36661 gcagattatg gtttataccc acgactcaat tggcctgggc gaagatggtc cgacgcacca
    36721 ggctgttgag caactggcca gcctgcgctt aacgccaaat ttcagcacct ggcgaccgtg
    36781 cgatcaggtg gaagcggcgg tgggctggaa gctggcggtt gagcgccaca acggaccgac
    36841 ggcactgatc ctctcaaggc agaatctggc ccaggtggaa cgtacgccgg atcaggttaa
    36901 agagattact cgtggcggtt atgtgctgaa agacagcggc ggtaagccag atattattct
    36961 gattgccacc ggttcagaga tggaaatcac cctgcaagct gcggagaaat tagcaggaga
    37021 aggtcgcaat gttcgcgtgg tttccctgcc ctcgaccgat attttcgacg cccaggatga
    37081 ggaatatcgg gagtcggtgt tgccttctaa cgttgcggct cgcgtggcgg tggaagcagg
    37141 tattgccgat tactggtaca agtatgttgg tctgaaaggg gcaattgtcg ggatgacggg
    37201 ttatggggaa tctgctccgg cggataagct gttcccgttc tttggcttta ccgccgagaa
    37261 tattgtggca aaagcgcata aggtgctggg agtgaaaggt gcctgatagt gatcgccgga
    37321 tgctgattgc cggatgcgac gctgacgcgt cttatccggc ctacagcgtc ttatccggcc
    37381 tacatgtccc cgccattttg tttaacgggt gatccacaac gtgggccagg cgtctggccc
    37441 atgccagtta tcgcaggtgg gttctgcggc gtaacgcacc aggcgaaaac gctgaccgtc
    37501 aaagcgccag cgcgcctgaa tgccacaatc gcttaatccg cgccctttcg ctaaggtcac
    37561 cagttcacgc gatttctcat caaatgttgc gttcatcagt tccagttcat tcgtctcctg
    37621 cccgttgttg aacggcaaac gcaaccgaac cgggcgcgag gctagcggct ttttacgcga
    37681 cacaatccat gccaaatcaa tggtgttata ggcccctgcc tcacagctga tcatcatcag
    37741 cgctttatca tcagtcagcg cagtgacatt cacctcacga cgtaacggat caagcgagca
    37801 gcgcagacca ttcatccgcc agtttccata atccagcaaa tcgttgcgtt cttcgagtga
    37861 gagtggcgtc ggcgttgggt taaccaccgc gacctctttc agcgcaggcg cgggcggtac
    37921 gctgagcggc ggttcgtccc ctttcttgat ccacgcggtt tcactgccaa cgcgcttttg
    37981 ctgagcatca ataaacaaca acgccgcttt taagccactc agagaaatgg tctgattgcc
    38041 gtcgcgtaag gtgatcgcct tcccttcctg aatcatctgc aaaaacgcgg tgatggttgc
    38101 cgtatcatcg gttactaata accatggtga aatccgccac ttgtcaccac ttaacgccaa
    38161 cggctcacca tctaacagca gccgtggcgc tatctctcct tcagacgcat ccggcgactt
    38221 caacccgccg cgctcaatac gtaaaacggc atcggtatgc gccccggcgc tgcggctcag
    38281 ggtcatcacc agtccattat gatcgcccgt attgcgcgcc acgcagaagt tttggttatt
    38341 acaggtgacc tgccagtcgg aaaacgcccg ttgcgctagt gccgcccaca ccaaagacgt
    38401 cggcaacaaa gcgaaaaaaa agagaaggaa aatgcgatag cgcatggacg gtacgacccc
    38461 agaagttcaa acaaacagca caagtcgtat cttcgttgcg caggtagcgc ggctcaatca
    38521 gatttgtcgg attgagccaa taataaacaa cgaaatatta ttgttatttt tcagtccatt
    38581 aaatgtgacg tttgcaaata gttaagcaat aacaccgtct taccgtcacg tatctcgccg
    38641 gttttgatca tctccagcgc ctggctgaac ggcagctcga gcacttcaat atcttcatct
    38701 tcaacaccgc caccggcgtt agcgcgctga ttgtcactgt attcggcgat aaaaaagtgg
    38761 attagctcag tcacaccgcc aggcgacata tacagttcaa ataatttgcg cacttcgccc
    38821 acttcatagc ccgtctcttc aatcgcttct ttacgaatgc acacttccgg ttcgtcgtta
    38881 tccagcagcc cggcgcaggt ttcaatcagc tgcccgcttt cattgccatt aacccaggta
    38941 gcgacacgga actgacgaat cagaaccacg gtctttttct tcgcgttgta caggaggatc
    39001 gtcgcaccat taccgcgatc gtaaacttca cgtttatggc ggataactta gccatctttg
    39061 cgcgtgagat cgtaagtaat gttgtgcagg gtgaaatagt tatcggagag aattttgtct
    39121 ttaatgaggg tgatttgttg cgtcataccg actccacagc gcgaaatgaa caattatatt
    39181 acgctgtgaa gtcgggtttg tctgcgcagg ctatcagtga gatttgactg attttacccc
    39241 cagccagtca ataatcccct gtgccgcatg acgtccttct gccattgcgg taaccaccag
    39301 atccgcacca cgcacggcgt cgccaccggc gaagattttc ggattggtgg tctggtaacg
    39361 gtactggctt tccacatccg cgatgatgcg gccccatttg tctaccgtta caccgtgcga
    39421 ctccagccac ggcatcccgt gtggattgaa gccaaacgcc ataatcaccg catcggctgg
    39481 catgacaaat tcactgccct caatcggtac aagtcgccgg cgcccctggg catccggctc
    39541 gccaagacgc gtgcgcagga aacgaattcc gcaaacgtga ccttgctcat tcagctcaag
    39601 cgccaccggc tggacgttaa attcgaagtt ggccccctct tcgcgggcgt tcttcacctc
    39661 tttcttcgag cctggcatgt tagcttcatc acgacgataa gcgcaggtga cgttactcgc
    39721 gccgtggcgc agcgcggtac gcacacagtc catcgcggtg tcgccgccgc ccagtaccac
    39781 gacgttgagt ccggcggtat tgataaacgg ctcttccggt agctcttcga gtcccatcac
    39841 ctgtttagtg ttggcgatga ggaacggcag cgcgtcataa acgcccggcg catcttcatt
    39901 gggtaaaccc gctttcatgg aacggtaagt gcctacgcca acgaagaccg cgtcgtattg
    39961 ttccaaaagc gaatccaaag agacatcttt acccacttca caattgagtt cgaagtgaat
    40021 ccccatcgcg ctgaagattt cccgacggcg tgccagcagg gatttatcca gtttgaaaga
    40081 aggaatgccg aaagtgagca agccaccaat ttctggatgg cgatcgtaca ccgtcaccgc
    40141 cacgccattg cgggtcagaa catccgcaca ggccagccct gccggacccg caccgataat
    40201 cgccacccgc ttgtccactt tggtgacatg gcttaagtca ggacgccaac ctttcgccaa
    40261 cgcctgatct gaaatgtagc gttcaatgtt gccgatagtt accgcgccgt gctcatcgcg
    40321 aatagtacag gcaccttcac acaaacggtc ctgcggacaa acgcgtccgg taatttccgg
    40381 cagggtgttg gtcaggtgag aaagctcgac ggcggcgtcg atgtttccgg ctttcaccag
    40441 ttcaatccac tgcggaatat ggttatgcag cgggcaggtc cattcacaaa cgctgtgctc
    40501 gccgcattta aggcagcgcg aggcttcccg ttgtgcctga tcggcacgga atggcagata
    40561 aatttcatca aaaccggttt tgcgcgcttc aatcgccagt ttatccggct cgccacgcgc
    40621 gggcgttgcc tgcatttgtt cgactttact cattaccggc atttcttgtg cagcggtact
    40681 ggcatgccac ggttgatgct cctgacgtgc ggtgcgcaag cggcgggatt tcgccatgcc
    40741 ggagagtgcg acgtcagtga ccagttgcag cgcgtccgcc gggcaattct cgacacaggc
    40801 aggaccgttt tcgcgccctg cacaaaggtc gcatttatgc gccgtggctt ttacttttcc
    40861 tgccgcgacg ggtgtcagga cgatttgcat cgtaccaaac ggacaggcca ccacgcagga
    40921 tttacagcca atacactttt gctgattgac ctgaatgctg tcatcaacgt ggctgattgc
    40981 gccattaggg cagctacggg cgcagggcgc atcttcacaa tggtgacagg tcactgcact
    41041 acgttgctgc tgatgtttga taaccgtaat tcggggatga aaatggtgtt ggctcaggac
    41101 atgttgctca tcattgtgag ccatgacaca ggcgatttca caagcatgac aacccagaca
    41161 ttgctgactg ttggccataa taaaacgatt cataacgacc ttcttttttg gttgtaaaaa
    41221 ccttattctt tatatgagtg ttgttattac ccgacttaca ggggaatcgg caatgttcat
    41281 gtgcccagaa taagtaacta tttcgctata aactgtggca gatcaaataa tcccatcact
    41341 gactaaattg cgttttagcg aactggaaca ttaatgattt tttgtggaga agacgcgtgt
    41401 gattgttaaa cgacccgtct cggccagtct ggcccgggcc tttttttaca ttgtgctgct
    41461 gtcgattctt tccacgggta tcgctctgct aactctggcg agcagtttgc gcgacgctga
    41521 ggctatcaat attgccggat cgctgcgtat gcagagttac cgcctgggct acgacttgca
    41581 aagtggcagt ccacaactca atgcacatcg ccagttgttt cagcaggcac tgcattcatc
    41641 ggtattaacc aatctcaacg tctggtatgt gccagaagca gtaaaaactc gctatgcgca
    41701 tctgaatgcc aactggctgg agatgaataa tcggctcagc aagggcgatt tgccgtggta
    41761 tcaggccaat attaataatt atgttaatca gatagacctg ttcgtgctgg ctttacagca
    41821 ctacgctgaa cgcaaaatgc tgctggtggt ggcgatttcc ctggctggcg gtatcggtat
    41881 tttcacgctg gtctttttta ctttgcgccg tatacgccat caggtggttg ccccgctgaa
    41941 tcagctggtt accgccagtc aacgtattga acacgggcag ttcgactcgc cgccgctgga
    42001 taccagtctg ccgaatgagc ttggtctgct tgcaaaaacc tttaaccaga tgtcgagcga
    42061 gctgcataaa ttgtaccttt cgctggaggc gtcagtagaa gaaaagaccc gcgatctcca
    42121 cgaggccaag cgtcgtctgg aggtgttgta tcaatgttcg caggcgctga acacaagcca
    42181 gattgatgtg cattgtttcc gccatatttt gcagattgtt cgcgacaatg aagcggctga
    42241 atatctggag ttaaatgtcg gtgaaaactg gcggattagc gaagggcaac caaacccgga
    42301 attgccgatg cagattttac cggtgacaat gcaagagacg gtttacggcg aactgcactg
    42361 gcaaaatagt cacgtttcat catcagaacc gctgcttaac agcgtttcgt cgatgctggg
    42421 acgcggtttg tactttaatc aggcgcaaaa gcattttcag cagttattgt tgatggaaga
    42481 acgtgcaacc atcgcccgcg aattgcacga ctcgctggct caggtacttt cttacttacg
    42541 tatccagttg acgttactga agcgttcgat accggaagat aacgccaccg cacaaagtat
    42601 catggccgat ttttcccagg cgttgaatga tgcttatcgg cagttacgcg agctgttgac
    42661 tactttccgc ctgacgctgc agcaggcgga tctcccctcc gcgttgaggg aaatgctgga
    42721 tacgttacaa aatcaaacca gcgccaaact gaccctcgac tgccgtctgc caaccctggc
    42781 gctggatgcg caaatgcagg tgcatttgtt gcaaattatt cgcgaagcgg tgctgaatgc
    42841 gatgaagcac gccaacgcca gcgaaatcgc cgtcagttgc gtcaccgcgc cggacggcaa
    42901 tcacacggtt tatatccgtg ataacgggat tggtatcggt gaaccgaaag aacccgaagg
    42961 tcattatggt ctgaatatca tgcgcgaacg cgcggaacgg ctgggtggga cgctgacttt
    43021 ttcacaacct tccggcggcg gcacgttagt gagtattagc tttcgctctg cggagggtga
    43081 ggaaagtcag ttgatgtaat gcctcctact gaccaaagaa tacttgcact taaggttcag
    43141 tataaaaggg catgataatt tacattaact cctttttttc tccacgattg gctcgtacct
    43201 tgccgctaca gtgaagcaag tcaagcctac aacgatacgc agaaacacga ggtcctcttt
    43261 taatggcgaa tttctttatt gatcgcccca tttttgcctg ggtgctggca atcctgttgt
    43321 gtctgacagg gaccctggcg attttttcat tgcccgttga acaatacccc gatctcgcgc
    43381 caccgaatgt gcgagtgacc gctaactatc ccggcgcatc ggcccagacg ctggaaaaca
    43441 ccgtgaccca ggttatcgag caaaatatga ccggcctcga taatctcatg tatatgtcat
    43501 ctcagagcag tggcatcggt caggcatctg tcactttaag ttttaaagca ggcaccgatc
    43561 cggacgaagc cgtgcagcaa gtacaaaacc agctgcaatc agccatgcga aagttaccgc
    43621 aggcggtgca aaatcagggc gtgacggtgc gtaaaaccgg cgataccaat attctgacca
    43681 ttgctttcgt ctctaccgat ggttcgatgg ataaacagga tattgccgat tatgttgcca
    43741 gtaatattca ggacccgtta agccgcgtga atggcgtcgg ggatatcgat gcctatggtt
    43801 cgcaatattc catgcgtatc tggctggacc cggcgaaact caacagtttc cagatgacgg
    43861 ctaaagatgt cactgatgcc attgagtcac agaacgcgca gattgcggtt gggcaacttg
    43921 gtggtacacc ttccgtcgat aagcaggcgc tcaacgccac cattaacgcc cagtcactgc
    43981 tgcaaacacc agaacagttc cgcgatatca ccttgcgggt gaatcaagac ggctcagagg
    44041 taaggctggg cgatgtcgcc accgtcgaaa tgggggcgga gaaatacgat tatcttagcc
    44101 gcttcaatgg taagccagcc tccgggctgg gggtaaaact ggcctccggc gcaaacgaaa
    44161 tggcgacagc ggagctggtg ctcaatcgtc tcgacgagct ggcgcagtac ttcccgcacg
    44221 gactggaata caaggtggcg tatgaaacca cctcgtttgt taaagcctcc attgaagacg
    44281 tggtgaaaac gctgctggaa gctatcgccc tggttttcct cgttatgtat ctgttcctgc
    44341 aaaacttccg cgccacgctg atacccacta tcgccgtgcc ggtggtgttg atgggaacct
    44401 tctccgtact ttacgccttc ggttacagcg tcaacacctt aaccatgttc gcgatggtac
    44461 tggcgatcgg tctgctggtg gatgacgcaa tcgtggtggt ggaaaacgtc gaacgtatta
    44521 tgagtgagga aggactcact cctcgcgaag ccacacgtaa atcgatgggg cagatccagg
    44581 gggcactggt cgggattgcg atggtgcttt cggcggtatt tgtaccaatg gccttcttcg
    44641 gcggcaccac cggtgccatc tatcgccagt tctctattac cattgttgcg gcgatggtgc
    44701 tgtcagtact ggtagcgatg atcctcactc cggctctgtg tgccacacta cttaagccac
    44761 tgaaaaaagg tgagcatcac gggcaaaaag gcttttttgc ctggtttaac cagatgttta
    44821 accgcaacgc cgaacgctac gaaaaagggg tggcgaaaat tctccaccgc agcctgcgct
    44881 ggattgtgat ttatgtcctg ctgcttggcg gcatggtgtt cctgttcctg cgtttgccga
    44941 cgtcgttctt accgctggaa gaccgtggca tgtttactac ctcggtacaa ttgcccagcg
    45001 gttcaaccca acaacagacc ctgaaagtcg ttgagcaaat cgagaaatat tacttcaccc
    45061 atgaaaaaga caacatcatg tcggtgtttg ccaccgttgg ttctggccct gggggtaacg
    45121 ggcaaaacgt ggcgcgaatg tttatccgcc tgaaagactg gagcgaacgc gacagtaaga
    45181 ccggcacctc gtttgccatt attgagcgtg caacaaaggc gtttaacaag attaaagaag
    45241 ctcgcgttat cgccagcagc ccgccagcaa ttagcggtct tggtagttct gcgggttttg
    45301 atatggagtt gcaggaccac gctggagcag gtcacgatgc gctgatggca gcacgtaacc
    45361 agttgctggc gctggcggcg gaaaacccgg agctaacccg tgtgcgccat aacggcctcg
    45421 acgacagtcc gcagttgcag attgatatcg accagcgtaa agctcaggcg ctgggcgttg
    45481 ctatcgacga tattaacgac acactgcaaa ccgcctgggg ttcgagctat gtgaatgact
    45541 ttatggatcg cggtcgcgtg aagaaagtct atgtgcaggc agctgcgccg tatcgcatgc
    45601 tgccagatga catcaatctc tggtatgtcc gaaataaaga tggcggcatg gtgcccttct
    45661 ctgctttcgc gacctcacgc tgggaaacag gctcgccgcg tctggaacgc tataacggtt
    45721 attctgcggt tgagattgtt ggggaagccg caccgggggt cagtaccggt acggcgatgg
    45781 atattatgga atcgttagtg aagcagctgc caaacggctt tggtctggag tggacggcga
    45841 tgtcgtatca ggagcgactt tccggcgcgc aggctccggc gctgtacgcc atttccttgc
    45901 tggtggtatt cctgtgtctg gctgcgttgt atgaaagctg gtcggtgccg ttctcggtaa
    45961 tgctggtcgt gccgctgggg gtaatcggcg cgctgctggc aacctggatg cgcgggctgg
    46021 aaaacgacgt ttacttccag gtgggcctgt taacggtcat tggtttatcg gcgaaaaacg
    46081 ccatcctgat cgtcgagttt gctaacgaga tgaaccaaaa aggccacgac ctgtttgaag
    46141 cgacgctcca cgcctgccgt cagcgtttac gcccgattct gatgacctcg ctggcattta
    46201 tcttcggcgt attgccaatg gcaaccagca cgggtgccgg ttccggtggt cagcatgcgg
    46261 tgggtactgg cgtaatgggc gggatgattt cagccactat tctggctatt tacttcgtgc
    46321 cgctgttctt tgtgctggtg cgccgccgct tcccgctaaa gccgcgcccg gaataagcaa
    46381 taaaaaaggc gacatgccaa tgtgtcgcct ttttcaactt tccgattaag aacctgctca
    46441 gcgggttctt gctgtttata ctttgtctca ggaattactt acgaagcata acttcgataa
    46501 agtctttcca gttccccagt tcacgttcaa tcataacaac ctctcttata attatgggta
    46561 ttctacggaa acaatatacc gtggtgaagc taatttactc gattgctgcg atgactacct
    46621 ccggggggac aaatcttatg taaatactat ggtcctgcgg tgatgatttg tatgtgatac
    46681 acagcaacat ttcgagatat tcatacggca tctaatac
//
LOCUS       DAZPDU010000010        46240 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZPDU010000010 DAZPDU010000000
VERSION     DAZPDU010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46240
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.010"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(324..536)
                     /gene="rpmE"
                     /locus_tag="ACHURS_000505"
     CDS             complement(324..536)
                     /gene="rpmE"
                     /locus_tag="ACHURS_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFR1236323.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            739..2937
                     /gene="priA"
                     /locus_tag="ACHURS_000506"
     CDS             739..2937
                     /gene="priA"
                     /locus_tag="ACHURS_000506"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFR1236324.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMAVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQTLRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3093..4118
                     /gene="cytR"
                     /locus_tag="ACHURS_000507"
     CDS             3093..4118
                     /gene="cytR"
                     /locus_tag="ACHURS_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFR1236325.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4210..5169
                     /gene="ftsN"
                     /locus_tag="ACHURS_000508"
     CDS             4210..5169
                     /gene="ftsN"
                     /locus_tag="ACHURS_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFR1236326.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5262..5792
                     /gene="hslV"
                     /locus_tag="ACHURS_000509"
     CDS             5262..5792
                     /gene="hslV"
                     /locus_tag="ACHURS_000509"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFR1236327.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5802..7133
                     /gene="hslU"
                     /locus_tag="ACHURS_000510"
     CDS             5802..7133
                     /gene="hslU"
                     /locus_tag="ACHURS_000510"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFR1236328.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7200..8126
                     /gene="menA"
                     /locus_tag="ACHURS_000511"
     CDS             7200..8126
                     /gene="menA"
                     /locus_tag="ACHURS_000511"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFR1236329.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLVLITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8219..8704
                     /gene="rraA"
                     /locus_tag="ACHURS_000512"
     CDS             8219..8704
                     /gene="rraA"
                     /locus_tag="ACHURS_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFR1236330.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8789..9034)
                     /gene="zapB"
                     /locus_tag="ACHURS_000513"
     CDS             complement(8789..9034)
                     /gene="zapB"
                     /locus_tag="ACHURS_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFR1236331.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9460..10305
                     /gene="glpF"
                     /locus_tag="ACHURS_000514"
     CDS             9460..10305
                     /gene="glpF"
                     /locus_tag="ACHURS_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFR1236332.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10328..11839
                     /gene="glpK"
                     /locus_tag="ACHURS_000515"
     CDS             10328..11839
                     /gene="glpK"
                     /locus_tag="ACHURS_000515"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFR1236333.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDCTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDVM"
     gene            11969..12979
                     /gene="glpX"
                     /locus_tag="ACHURS_000516"
     CDS             11969..12979
                     /gene="glpX"
                     /locus_tag="ACHURS_000516"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFR1236334.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            13076..13822
                     /gene="fpr"
                     /locus_tag="ACHURS_000517"
     CDS             13076..13822
                     /gene="fpr"
                     /locus_tag="ACHURS_000517"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFR1236335.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLEE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13827..14255)
                     /gene="uspD"
                     /locus_tag="ACHURS_000518"
     CDS             complement(13827..14255)
                     /gene="uspD"
                     /locus_tag="ACHURS_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFR1236336.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14282..14581)
                     /locus_tag="ACHURS_000519"
     CDS             complement(14282..14581)
                     /locus_tag="ACHURS_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFR1236337.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14793..15233)
                     /locus_tag="ACHURS_000520"
     CDS             complement(14793..15233)
                     /locus_tag="ACHURS_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR1236338.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15334..15933
                     /locus_tag="ACHURS_000521"
     CDS             15334..15933
                     /locus_tag="ACHURS_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFR1236339.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            16041..16808
                     /gene="tpiA"
                     /locus_tag="ACHURS_000522"
     CDS             16041..16808
                     /gene="tpiA"
                     /locus_tag="ACHURS_000522"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFR1236340.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16863..17618)
                     /gene="cdh"
                     /locus_tag="ACHURS_000523"
     CDS             complement(16863..17618)
                     /gene="cdh"
                     /locus_tag="ACHURS_000523"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFR1236341.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(17725..18714)
                     /gene="sbp"
                     /locus_tag="ACHURS_000524"
     CDS             complement(17725..18714)
                     /gene="sbp"
                     /locus_tag="ACHURS_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFR1236342.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(19034..19996)
                     /gene="pfkA"
                     /locus_tag="ACHURS_000525"
     CDS             complement(19034..19996)
                     /gene="pfkA"
                     /locus_tag="ACHURS_000525"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFR1236343.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20177..21079)
                     /gene="fieF"
                     /locus_tag="ACHURS_000526"
     CDS             complement(20177..21079)
                     /gene="fieF"
                     /locus_tag="ACHURS_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFR1236344.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21314..21532)
                     /gene="ogrK"
                     /locus_tag="ACHURS_000527"
     CDS             complement(21314..21532)
                     /gene="ogrK"
                     /locus_tag="ACHURS_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage transcriptional regulator OgrK"
                     /protein_id="HFR1236345.1"
                     /translation="MFHCPLCQHAAHARTSRYITDTTKERYHQCQNVNCSATFITYES
                     VQRYIVKPGEVHAVRPHPLPSGQQIMWM"
     gene            complement(21614..22777)
                     /locus_tag="ACHURS_000528"
     CDS             complement(21614..22777)
                     /locus_tag="ACHURS_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFR1236346.1"
                     /translation="MLDALTFDAGSTLTPDYMLMLDSRDITGNISDRLMSMTLTDNRG
                     FEADQLDIELNDADGQVGLPVRGAVLTVYIGWKGFALVCKGKFTVDEVEHRGAPDVVT
                     IRARSADFRGTLNSRREGSWHDITLGAIVEAIASRNRLEASVAPSLAGIKIPHIDQSQ
                     ESDAKFLTRLAERNGGEVSVKMGKLLFLKAGQGVTASGKKIPQITITRSDGDRHHFAI
                     ADRGAYTGVTAKWLHTKDPKPQKQKVKLKRKKKEKHLRALEHPKAKPVTQKKAPKVPE
                     AREGEYMAGEADNVFALTTVYATKAQAMRAAQAKWDKLQRGVAEFSISLATGRADIYT
                     ETPVKVSGFKRVIDEQDWTITKVTHCLNNSGFTTSLELEVRLSDVEYETEDDE"
     gene            complement(22777..23256)
                     /locus_tag="ACHURS_000529"
     CDS             complement(22777..23256)
                     /locus_tag="ACHURS_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1236347.1"
                     /translation="MMLALGMFVFMRQTLPHQTMQRESDYRWPSNSRIGKRDAYQFLG
                     VGEENITLAGVLYPELTGGKLTMTTLRLMAEEGRAWPLLDGTGMIYGMYVISKVSETG
                     SIFFADGTPRKIDFTLSLTRVDESLAALYGDIGKQAESLIGKAGSMATKFTGMTGAG"
     gene            complement(23271..25718)
                     /locus_tag="ACHURS_000530"
     CDS             complement(23271..25718)
                     /locus_tag="ACHURS_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR1236348.1"
                     /translation="MSNNVKLQVLLRAVDQASRPFKSIRRASKLLSGDIRETQKSLRE
                     LNGHASRIEGFRKTSAQLAVTGHALEKARQEAEALATQFKNTERPTRAQAKVLESAKR
                     AAEDLQAKYNRLTDSVKRQQRELAAVGINTRNLAHDEQGLKNRISETTAQLNRQRDAL
                     ARVSAQQAKLNAVKQRYQAGKELAGNMASVGAAGVGIAAAGTIAGVKLLMPGYEFAQK
                     NSELQAVLGVAKDSAEMAALRKQARQLGDNTAASADDAAGAQIIIAKAGGDVDAIQAA
                     TPVTLNMALANRRTMEENAALLMGMKSAFQLSNDKVAHIGDVLSMTMNKTAADFDGMS
                     DALTYAAPVAKNAGVSIEETAAMVGALHDAKITGSMAGTGSRAVLSRLQAPTGKAWDA
                     LKELGVKTSDSKGNTRPVFTILKEMQASFEKNRLGTAQQAEYMKTIFGEEASSAAAVL
                     MTAASTGKLDKLTAAFKASDGKTAELVNIMQDNLGGDFKEFQSAYEAVGTDLFDQQEG
                     ALRKLTQTATKYVLKLDGWVQKNKSLASTIGLIAGGALALTGIIGAIGLVAWPVITGI
                     NAIIAAAGAMGAIFTTVGSAVMTAIGAISWPVVAVVAAIVAGALLIRKYWEPVSAFFG
                     GVVEGLKAAFAPVGELFTPLKPVFDWLGEKLQAAWQWFKNLIAPVKATQDTLNRCRDT
                     GVMFGQALADALMLPLNAFNKLRSGIDWVLEKLGVINKESDTLDQTAARTHAATYGTG
                     GYIPATSSYAGYQAYQPVTAPAGRSYVDQSKNEYHISLTGGTAPGTQLDRQLQDALEK
                     YERDKRARARASMMHDG"
     gene            complement(25711..25866)
                     /locus_tag="ACHURS_000531"
     CDS             complement(25711..25866)
                     /locus_tag="ACHURS_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFR1236349.1"
                     /translation="MTLPKNLSVDDLMADVAVIFHWPPSELYPMSLTELITWREKALR
                     RSGNTNE"
     gene            complement(25863..26138)
                     /locus_tag="ACHURS_000532"
     CDS             complement(25863..26138)
                     /locus_tag="ACHURS_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFR1236350.1"
                     /translation="MNKENVITLDNPVKRGEQVIEQVTLMKPSAGTLRGVSLAAVANS
                     EVDALIKVLPRMTAPMLTEQEVAALELPDLVALAGKVVGFLSPNSVQ"
     gene            complement(26195..26713)
                     /locus_tag="ACHURS_000533"
     CDS             complement(26195..26713)
                     /locus_tag="ACHURS_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFR1236351.1"
                     /translation="MAMPRKLKLMNVFLNGYSYQGVAKSVTLPKLTRKLENYRGAGMN
                     GSAPVDLGLDDDALSMEWSLGGFPDSVIWELYAATGVDAVPIRFAGSYQRDDTGETVA
                     VEVVMRGRQKEIDTGEGKQGEDTESKISVVCTYFRLTMDGKELVEIDTINMIEKVNGV
                     DRLEQHRRNIGL"
     gene            complement(26726..27916)
                     /locus_tag="ACHURS_000534"
     CDS             complement(26726..27916)
                     /locus_tag="ACHURS_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HFR1236352.1"
                     /translation="MSDYHHGVQVLEINDGTRVISTVSTAIVGMVCTASDADAETFPL
                     NKPVLITNVQSAIAKAGKKGTLAASLQAIADQSKPVTVVVRVEDGTGDDEETKLAQTV
                     SNIIGTTDENGQYTGLKALLAAESVTGVKPRILGVPGLDTKEVAVALASVCQKLRAFG
                     YISAWGCKTVSEVKAYRQNFSQRELMVIWPDFLAWDTVASTTATAYATARALGLRAKI
                     DQEQGWHKTLSNVGVNGVTGISASVFWDLQESGTDADLLNESGVTTLIRRDGFRFWGN
                     RTCSDDPLFLFENYTRTAQVLADTMAEAHMWAVDKPITATLIRDIVDGINAKFRELKT
                     NGYIVDATCWFSEESNDAETLKAGKLYIDYDYTPVPPLENLTLRQRITDKYLANLVTS
                     VNSN"
     gene            28327..29745
                     /locus_tag="ACHURS_000535"
     CDS             28327..29745
                     /locus_tag="ACHURS_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1236353.1"
                     /translation="MTYALIDNSTLTAVQRIEGKVKTRSKDSVDTDIIAFENYIQSIL
                     FYDHVIAVDDYIPEYRGSRISSFREILFLDKAQYGLDEIESEAKKISDALKPKIRGGT
                     FVNDDFKKLIELLQTHIICTWDISSSVYHLTLKNLSDGGKEFEKYGNIAAAIFSELRD
                     AAESGNRTNGDVELLDRFGNPIRKGYKVPGAKWGDGTSAGEASDAIKAFVASLVWLAN
                     RAVFYSLTAKYLQADTFLYPLRQAYQQTYLSQNCQYGFDYAKHIVENFSTSLSHDLID
                     IHSGGKAVATATSLPLFSAWLAKETGDPSCIVEAAYNIRDNNEFVRAREKMREIKRLF
                     DENELPQANKSVRALLTEVSKESDYIRTKYGLKTRQGVPVTKLVHVYNTVAAINSLPK
                     LPTFNFKVKVPDFLYDLKKPKGFRALYRNLTNDLSTVWSLGEARDILGSKVVIDNHAR
                     IYSPKHEEPRFKNSHSPYKSPM"
     gene            complement(29860..30387)
                     /locus_tag="ACHURS_000536"
     CDS             complement(29860..30387)
                     /locus_tag="ACHURS_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR1236354.1"
                     /translation="MQHLINITAGNPKTVEQYQLTKDFDVVWFFSEDGKNWYEEQKYF
                     ADDTIKIAYDKDNIIRYVEKDVTAIRPDGLSVVEVPDITANRRADISGNWMFKDCTVI
                     KRIYTAEELQQQAENRKARLLADAESVILPLERAVRLNMATDEERSRLEAWERYSVLV
                     SRVDPANPEWPEMPQ"
     gene            complement(30391..32709)
                     /locus_tag="ACHURS_000537"
     CDS             complement(30391..32709)
                     /locus_tag="ACHURS_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001623412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFR1236355.1"
                     /translation="MSTKFRTVITTAGAAKLAAATAPGGRKVNITTMAVGDGGGKLPV
                     PDAGQTGLIHEVWRHTLNKISQDKRNSNYIIAELVIPPEVGGFWMRELGLYDDAGTLI
                     AVANMAESYKPALAEGSGRSQTCRMVIIVSSVASVELTIDTTTVMATQDYVDDKIAEH
                     EQSRRHPDASLTAKGFTQLSSATNSTSETLAATPKAVKAAYDLANGKYTAQDATTARK
                     GLVQLSSATNSTSETLAATPKAVKTAYDLANGKYTAQDATTARKGLVQLSSATNSDSE
                     TLAATPKAVKTAYDLANGKYTAQDATTARKGLVQLSSAINSDSETLAATPKAVKSAYD
                     NAEKRLQKDQNGADIPGKDTFTKNIGACRAYSGALSTEAGNWTTAQFIEWLDSRGAFN
                     HPYWMCKGSWSYANNKIITDTGCGDIHLAGCVVEVMGTKSAITIRVTTPTTSSGGGTT
                     SAQFTYINHGDGYSPGWRRDWNRQGDSMTGTINQDGGSQNAYMSTALCSGTRGGKKYL
                     RKFRGGEGDTIWHETVQGGVIRWATGNNDAQEELSLSSAYGLRSRGEITSLSANGLRI
                     AYGNYGFFIRNDGGSTYLMLTASGDKFGTWNGLRPLTINNANGGVSMGHGLSVTGDIV
                     SSTKVRAGSGKKFTVSSSNTSTKEAAFNLWGNSSRPVVAELGDDAGWHFYSQRNTDNS
                     ITFAVNGQVSPSNYGNFDSRYVRDIRLGGAATYKPANNGMTWTHQAPSGCVYSGIIVQ
                     DTGSNSADNIGGVYYRPVQKYINGTWYNVAQV"
     gene            complement(32720..33250)
                     /locus_tag="ACHURS_000538"
     CDS             complement(32720..33250)
                     /locus_tag="ACHURS_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFR1236356.1"
                     /translation="MSDSRLLPTGSSPLEVAAAKACAEIEKTPVRIRELWNPDTCPAN
                     LLPWLAWAFSVDRWDEKWPEATKRAVIRDAYFIHCHKGTIGAIRRVVEPLGYLINVTE
                     WWENSDPPGTFRLDIGVLESGITEAMYQEMERLIADAKPASRHLIGLNITRDIPGYLF
                     AGGVAYDGDVITVYPG"
     gene            complement(33243..34151)
                     /locus_tag="ACHURS_000539"
     CDS             complement(33243..34151)
                     /locus_tag="ACHURS_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HFR1236357.1"
                     /translation="MPIIDLNQLPAPDVVEELDFETILAERKATLISLYPEDQQEAVA
                     RTLTLESEPLVKLLEENAYRELIWRQRVNEAARAVMLACAAGNDLDVIGANYNTTRLI
                     ITPADDSTLPPTPAVMESDTDYRLRIQQAFEGLSVAGSVGAYQYHGRSADGRVADISV
                     TSPSPACVTISVLSRENNGVASEDLLAVVRNALNGEDVRPVADRVTVQSAAIVEYQIN
                     ATLYLYPGPESEPIRAAAVKKLEAYITAQHRLGRDIRLSAIYAALHVEGVQRVELTAP
                     LADIVLNSTQASFCTEYRVVTGGSDE"
     gene            complement(34156..34503)
                     /locus_tag="ACHURS_000540"
     CDS             complement(34156..34503)
                     /locus_tag="ACHURS_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFR1236358.1"
                     /translation="MTARYLGMNRSDGLTVSDLEHISQSIGDILRTPVGSRVMRRDYG
                     SLLASMIDQPQTPALELQIKVACYMAVLKWEARVTLSSVTTARSFDGRMTVTLTGQHN
                     DTGQPLSLTIPVS"
     gene            complement(34500..35135)
                     /locus_tag="ACHURS_000541"
     CDS             complement(34500..35135)
                     /locus_tag="ACHURS_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFR1236359.1"
                     /translation="MNTLANIQELARALRNMIRTGIIVETDLNAGRCRVQTGGMCTDW
                     LQWLTHRAGRSRTWWAPSVGEQVLILAVGGELDTAFVLPGIYSGDNPAPSASADALHI
                     RFPDGAVIEYEPETSALTVSGIKTASVTASDSVTATVPVVTVKASTRVTLDTPEVVCT
                     NKLITGTLEVQKGGTMRGNIEHTGGELSSNGKVLHTHKHPGDSGGTTGSPL"
     gene            complement(35202..35654)
                     /locus_tag="ACHURS_000542"
     CDS             complement(35202..35654)
                     /locus_tag="ACHURS_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFR1236360.1"
                     /translation="MNEFKRFEDRLTGLIESLSPSGRRRLSAELAKRLRQSQQRRVMA
                     QKAPDGTPYAPRQQQSARKKTGRVKRKMFAKLITSRFLHIRASPEQASMEFYGGKSPK
                     IASVHQFGLSEETRKDGKKIHYPARPLLGFTGEDVQMIEEIILAHLDR"
     gene            complement(35647..36114)
                     /locus_tag="ACHURS_000543"
     CDS             complement(35647..36114)
                     /locus_tag="ACHURS_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001406878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1236361.1"
                     /translation="MLKPDSLRRALTDAVTVLKTSPEMLRIFVDNGSIASTLATSLSF
                     EKRYTLNVIVTDFTGDFDLLIVPVLAWLRENQPDIMTTDEGQKKGFTFYADINNDSSF
                     DISISLMLTERTLVSEVDGALHVKNIPEPPPPEPVTRPMELYINGELVSKWDE"
     gene            complement(36077..36322)
                     /gene="lysC"
                     /locus_tag="ACHURS_000544"
     CDS             complement(36077..36322)
                     /gene="lysC"
                     /locus_tag="ACHURS_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979584.1"
                     /note="LysC is an Rz1-like component of a phage lytic
                     system, substantially overlapping although not fully
                     embedded in the gene for the Rz-like LysB component;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1-like lysis system protein LysC"
                     /protein_id="HFR1236362.1"
                     /translation="MLCAGCTSAQPAQTPVIVHNACPKVSLCPMPGSDPQTNGDLSAD
                     IRQLENALARCASQVKMIKHCQDENDAQTRQPAQGAD"
     gene            complement(36222..36647)
                     /gene="lysB"
                     /locus_tag="ACHURS_000545"
     CDS             complement(36222..36647)
                     /gene="lysB"
                     /locus_tag="ACHURS_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585210.1"
                     /note="The gene for this Rz-like phage lysis system
                     protein may overlap extensively with the gene for the
                     other spanin subunit, the Rz1-like protein in the outer
                     membrane; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz-like lysis system protein LysB"
                     /protein_id="HFR1236363.1"
                     /translation="MSKLMIVLVVLLSLAVAGLFLVKHKNASLRALLDRANNVASEQQ
                     TTITMLKNQLHVALTRADKNELAQVALRQELENAAKREAQREKTITRLLNENEDFRRW
                     YGADLPDAVRRLHQRTACADASDCPQRLPESEPLPDAGQ"
     gene            complement(36635..37060)
                     /locus_tag="ACHURS_000546"
     CDS             complement(36635..37060)
                     /locus_tag="ACHURS_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNZ54_00345 family protein"
                     /protein_id="HFR1236364.1"
                     /translation="MKKLSLSLMLNVSLALMLALSLIYPQSVAVSFVAAWAILATVIC
                     VIAGGVGVYAAEYVLERYGRELPPESLAVKIVTSLFLQPVPWRRRAAALMVMVATFIS
                     LVAAGWIFTALIYLVASLFFRLIRTACRQRLEGREPCQN"
     gene            complement(37075..37572)
                     /locus_tag="ACHURS_000547"
     CDS             complement(37075..37572)
                     /locus_tag="ACHURS_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HFR1236365.1"
                     /translation="MPVISTHQNIAAFLDMLAVSEGTANHPLTKNRGYDVIVTGLDGK
                     PEIFTDYSDHPFAHGRPAKVFNRRGEKSTASGRYQQLYLFWPHYRKQLALPDFSPLSQ
                     DRLAIQLIRERGALDDIRAGRIERAISRCRNIWASLPGAGYGQREHSLEKLVTVWRTA
                     GGVPA"
     gene            complement(37572..37853)
                     /locus_tag="ACHURS_000548"
     CDS             complement(37572..37853)
                     /locus_tag="ACHURS_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFR1236366.1"
                     /translation="MTAEEKSVLSLFMIGVLIVVGKVLAGGEPITPRLFIGRMLLGGF
                     VSMVAGVVLVQFPDLSLPAVCGIGSMLGIAGYQVIEIAIQRRFKGRGKP"
     gene            complement(37857..38060)
                     /locus_tag="ACHURS_000549"
     CDS             complement(37857..38060)
                     /locus_tag="ACHURS_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFR1236367.1"
                     /translation="MKTFALQGDTLDAICVRYYGRTEGVVEAVLAANPGLAELGAVLP
                     HGTAVELPDVQTAPVAETVNLWE"
     gene            complement(38060..38569)
                     /locus_tag="ACHURS_000550"
     CDS             complement(38060..38569)
                     /locus_tag="ACHURS_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFR1236368.1"
                     /translation="MMTLIIPRKEAPVSGEGTVVIPQPAGDEPVIKNTFFFPDIDPKR
                     VRERMRLEQTVAPARLREAIKSGMAETNAELYEYREQKIAAGFSRLADVPADDIDGES
                     IKVFYYERAVCAMATASLYERYRGVDASAKGDKKADSIDSTIDELWRDMRWAVARIQD
                     KPRCIVSQI"
     gene            complement(38669..39412)
                     /locus_tag="ACHURS_000551"
     CDS             complement(38669..39412)
                     /locus_tag="ACHURS_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HFR1236369.1"
                     /translation="MTSPAQRHMMRVSAAMTAQREAAPLRHATVYEQMLVKLAADQRT
                     LKAIYSKELKAAKKRELLPFWLPWVNGVLEQGKGAQDDILMTVMLWRLDTGDIAGALE
                     IARYALKYGLTMPGKHRRTPPYMFTEEVALAAMRAHAAGESVDPRLLTDTLELTATAD
                     MPDEVRAKLHKITGLFLRDAGDAAGALVHLQRATQLDCQAGVKKEIERLERELKPKPE
                     PQPKAATRTPRKTRSVTPAKRGRPKKKAS"
     gene            complement(39416..40488)
                     /locus_tag="ACHURS_000552"
                     /pseudo
     CDS             complement(39416..40488)
                     /locus_tag="ACHURS_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248534.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
     gene            complement(40547..41401)
                     /locus_tag="ACHURS_000553"
     CDS             complement(40547..41401)
                     /locus_tag="ACHURS_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFR1236370.1"
                     /translation="MAKKVSKFFRIGVEGDTCDGRVISAQDIQEMAETFDPRVYGCRI
                     NLEHLRGILPDGIFKRYGDVAELKAEKIDDDSALKGKWALFAKITPTDDLIAMNKAAQ
                     KVYTSMEIQPNFANTGKCYLVGLAVTDDPASLGTEYLEFCRTAKHNPLNRFKLSPENL
                     ISVATPVELEFEDLPETVFTALTEKVKSIFGRKQASDDARLNDVHEAVTAVAEHVQEK
                     LSATEQRLAEMETAFSALKQEVTDRADETSQAFTRLKNSLDHTESLTQQRRSKATGGG
                     GDALMTNC"
     gene            41575..43347
                     /locus_tag="ACHURS_000554"
     CDS             41575..43347
                     /locus_tag="ACHURS_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HFR1236371.1"
                     /translation="MTITTDTTLLHDPRRQAALLYWQGFSVPQIAAMLQMKRPTVQSW
                     KQRDGWDSVAPISRVEMSLEARLTQLIIKPQKTGGDFKEIDLLGRQIERLARVNRYSQ
                     TGNEADLNPNIANRNKGGRRKPKKNFFSDEAIEKLEQIFFEQSFDYQLHWYRAGLEHR
                     IRDILKSRQIGATFYFSREALLRALKTGHNQIFLSASKTQAYVFREYIIAFARLVDVD
                     LTGDPIVLGNNGAKLIFLGTNSNTAQSHNGDLYVDEIFWIPNFQVLRKVASGMASQSH
                     LRSTYFSTPSTLAHDAYPFWSGELFNRGRASAAERVEIDVSHNALAGGLLCADGQWRQ
                     IVTIEDALKGGCTLFDIEQLKRENSADDFKNLFMCEFVDDKVSVFPFEELQRCMVDTL
                     EEWEDYAPFAANPFGSRPVWIGYDPSHRGDSAGCVVLAPPVVAGGKFRILERHQWKGM
                     DFATQAESIRKLTEKYNVEYIGIDATGLGVGVFQLVRSFYPAARDIRYTPEMKTAMVL
                     KAKDVIRRGCLEYDVSATDITSSFMAIRKTMTSSGRSATYEASRSEEASHADLAWATM
                     HALLNEPLTAGISTPLTSTILEFY"
     gene            43347..44381
                     /locus_tag="ACHURS_000555"
     CDS             43347..44381
                     /locus_tag="ACHURS_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001752368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR1236372.1"
                     /translation="MSKKKGKTPRPAAKTITASAPKMEAFTFGEPVPVLDRRDILDYV
                     ECISNGRWYEPPVSFTGLAKSLRAAVHHSSPIYVKRNILASTFIPHPWLSQQDFSRFV
                     LDFLVFGNAFLEKRYSTTGKVIRLETSPAKYTRRGVEEDVYWWVPSFNEPTAFAPGSV
                     FHLLEPDINQELYGLPEYLSALNSAWLNESATLFRRKYYENGAHAGYIMYVTDAVQDR
                     NDIEMLRENMVKSKGRNNFKNLFLYAPQGKADGIKIIPLSEVATKDDFFNIKKASAAD
                     LLDAHRIPFQLMGGKPENVGSLGDIEKVAKVFVRNELIPLQDRIREINGWLGQEVIRF
                     KNYSLDTDNG"
     gene            44753..45895
                     /locus_tag="ACHURS_000556"
     CDS             44753..45895
                     /locus_tag="ACHURS_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFR1236373.1"
                     /translation="MDIIDLKSQLENEDNAKKRAELLAQLEVIKQNAEEQIRAEQKDV
                     DFETKEFTVELLVNKYHNGLEDDTNELFVPDYQRDFVWSEKRQSKLIESLILGFPIPY
                     IFIADVLSEDPDLDGRIEIVDGSQRVRTIHAFIQNELVLQDLKSLDVLNGFKFQDLPL
                     SRQRRFMRIPVRVIELSSKCNEETRRDLFERINSGSDILKDMEVRKGSELGSTNLYTQ
                     VIKPCSTIPKFKELAPLSQTKEKRDERLEFTLRFFAYVDKYKNFDHSVRDFLNDYMSE
                     NRDIDETKQNSMKQEFANVLSFVENYFPAGFKKTITAKSTPRVRFESLAVGAALALRE
                     VPDLVPKNLDWLSSEEFKRLTTSDGANSRVKVIERIEYVRDKLLED"
     gene            45897..>46240
                     /locus_tag="ACHURS_000557"
     CDS             45897..>46240
                     /locus_tag="ACHURS_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAE_28990/MAE_18760 family HEPN-like nuclease"
                     /protein_id="HFR1236374.1"
                     /translation="MDRFREDFDERSGEILAYLDLLKFIEYAGAELISADDKEHKFSI
                     TAQSRKTLKGAVYILLYNLIESTMREAICLIHETIYDRNVEFDKLRKNLRSEILKRLK
                     NESVNVESLVNHL"
BASE COUNT        10918 a        12410 c        11851 g        11061 t
ORIGIN      
        1 gcaccgagca gcggattgac cggatggccg gacgtcaccc cgccggggca caccgaacag
       61 gccacgccgg tgggggcacc gatgcgcacc ccggctgaac cctggacaat gctaccgcca
      121 tactgcgtca tgtcgccctg acgcgccgcc ggttttccgc tcatcgtaaa atccttttga
      181 aataaataag aaccagaagc ctagcgccag tcaacaacag gaaatgataa tgagttaaca
      241 ccccggaatt tttcagaatt tataaaaggc aaattacagg cacaaaaaaa gcgccgtgcg
      301 gcgctttttt cggaaatccg gtcttatttg ctgcccggga tgttgaaacg cttgttgaag
      361 cggtcaacac ggccaccggt agcaacatca cgctgtttgc cagtgaagaa cgggtggcac
      421 ttgctgcaca cgtcgaggtt caggtcatga ccaacggtgg agcggatttt cattacgtta
      481 ccgcaagagc agctagcagt aatttcttcg tatttcgggt gaatatcttt tttcatggga
      541 aaacctcggt ttaaggccgc gtcgctcttc cagccctaac gccagacacc acgcgatgtt
      601 aaaagtatag cttcaatacg atcatttcgt acgaaggcgc gaaatcatac agaaattaac
      661 cagcgtatgc aaactgatcc gcactcttct acggcaatgt gtatactaac ccaccgaatt
      721 tcaagtcagg atgatgctat gcccgttgcc cacgttgcct tgcccgttcc gcttcctcgt
      781 acctttgact atctgctgcc agaaggcatg gcggttaaag ccgggtgtcg cgtgcgcgtg
      841 ccgtttggca aacagcagga gcgcatcggg gttgtggtat cagttagcga tgtcagtgag
      901 ctgccgctta acgagctgaa agcggtagtt gaagtgctgg acgttgagcc ggtatttact
      961 cactccgtct ggcgattact gctctgggcg gcagattact atcatcatcc gattggcgat
     1021 gtgctgtttc atgccttgcc gattttactg cgccaggggc ggcctgcggc gaacgcgccg
     1081 atgtggtact ggtttgccac tgaacaaggc caggcggtgg atctgaacag cctgaaacgc
     1141 tcacccaagc aacaacaggc gctggcggcg ttacggcaag ggaaaatctg gcgcgaccag
     1201 gttgccacgc tcgaatttaa tgatgccgcg ttgcagacgc tgcgcaaaaa aggtctgtgt
     1261 gatttagcaa gtgaaacacc agagtttagc gactggcgaa cgaactatgc cgtttctggt
     1321 gagcggttgc gattgaatac cgaacaggcc accgccgttg gcgcaattca tagcgcggca
     1381 gatacttttt ctgcctggct gctggcgggc gttaccggtt ccggtaaaac ggaggtttat
     1441 ctcagtgtac tggaaaacgt gctcgctcag ggcaaacagg cgctggtgat ggtgccggaa
     1501 atcggcctga caccgcaaac tatcgcccgt tttcgtgaac ggtttaatgc gcccgtggaa
     1561 gttctgcatt ccggtctgaa cgacagcgag cgtctttcgg cgtggctgaa agcgaaaaat
     1621 ggcgaggcgg cgattgtgat cggcacccgc tccgcgctgt ttacgccgtt taaaaatctc
     1681 ggcgtgattg tcattgacga agagcacgac agttcctaca agcagcagga aggctggcgc
     1741 tatcatgccc gcgacctggc ggtgtatcgt gcgcatagcg agcaaatccc gattattctt
     1801 ggctccgcaa cgcccgcgct ggaaacgttg tgcaacgtcc agcagaaaaa ataccgcctg
     1861 ctgcgcctga cgcgccgggc ggggaatgcg cgtccggcaa ttcaacacgt gctggattta
     1921 aaaggtcaga aggtgcaggc aggtctggct ccggcgttaa tcacccgtat gcgccagcat
     1981 ttacaggcca acaatcaggt tatcctcttt cttaaccgcc gtggctttgc gcctgcgcta
     2041 ctgtgccacg actgcggctg gattgccgaa tgcccacgtt gcgatcacta ctacacgctg
     2101 catcaggcgc aacagcattt gcgctgtcac cactgtgaca gtcagcgtcc ggtgccgcgc
     2161 cagtgccctt cctgcggttc cacgcacctg gtacccgtgg ggctgggcac cgaacagctt
     2221 gaacagacgc ttgcgccgct gttccctgat gtgcccattt ctcgtatcga ccgcgatacc
     2281 accagccgca aaggggcgct ggaacagcaa ctggcggaag tacatcgcgg cggtgcgagg
     2341 attttgatcg gcacacaaat gctggcgaaa ggtcaccact ttccggatgt gacgctggtg
     2401 gcgttactgg atgtggacgg cgcgctgttt tctgccgatt ttcgctcggc agagcgtttc
     2461 gctcagcttt acacccaggt cgccggtcgt gccgggcgtg cgggtaaaca gggcgaagtg
     2521 gtgctgcaaa cgcaccatcc ggaacatcct ctgttgcaaa cgttgctcta taaaggctac
     2581 gacgcctttg ccgaacaggc gctggctgag cgacgcatga tgcagctgcc gccgtggacc
     2641 agccatgtga ttgtgcgtgc ggaagatcat aacaatcagc atgcgccgct gttcctgcaa
     2701 caactgcgta atctgatcct ctccagcccg ctggcagacg acaaattgtg ggttctcggt
     2761 ccggttccgg cactggcacc taaacgtggc ggtcgctggc gctggcagat attgttgcag
     2821 cacccttccc gcgtgcgctt gcaacacatc attaacggta cgctggcact catcaataca
     2881 ataccggatt cccgtaaggt gaaatgggtg ctggatgttg atccgattga gggttaaacc
     2941 gctcacgatg cgaggcggat cgaaaaattc aatattcatc acacttttca tgaaaattct
     3001 gtaaccgttt tcacgcgcta tctgctaaaa atgttgccga tgtgaagtaa acatggatgt
     3061 agtacgcctg acgtgccagg cgaggagtga gtgtgaaagc gaagaagcag gaaactgccg
     3121 cgaccatgaa agacgttgcc ctcaaggcaa aagtctctac agcgactgtc tcccgagcat
     3181 taatgaatcc cgataaagtc tcccaggcca cccgtaatcg ggttgaaaaa gcggcccggg
     3241 aagtgggtta tttaccgcag cctatggggc gcaacgtcaa gcgtaatgaa tcccgcacca
     3301 ttctggtgat tgtcccggat atctgcgatc ccttctttag cgaaattatt cgcggtatcg
     3361 aagttacggc ggcaaatcac ggatatctgg tgctgattgg cgactgtgcg catcaaaatc
     3421 agcaggaaaa aacctttatc gatttgatca tcaccaagca aattgatggc atgttgctgc
     3481 tgggttcaag gctgccgttt gatgccagca ttgaggaaca gcgtaatctg ccgccgatgg
     3541 tgatggcgaa cgaattcgca ccggaactgg agctacctac agttcatatc gacaatctga
     3601 ccgccgcatt tgatgcagta aattatctat atgagcaagg gcataaacgg attggctgta
     3661 tagccggtcc cgaagagatg ccgctgtgtc actaccgcct gcaaggttac gttcaggcgc
     3721 tgcgtcgctg cggcattatg gttgatccgc aatacatcgc ccgtggcgac ttcaccttcg
     3781 aagccggaag caaagcgatg cagcagctgc ttgatcttcc acaaccgcct actgctgtct
     3841 tctgccatag cgatgtgatg gcgctcggcg cactttctca ggcaaaacgc caggggctga
     3901 aagtcccgga agacctttcc ataatcggtt ttgataacat cgacctgacg caattttgtg
     3961 atccgccgct gacaaccatc gcgcagccgc gttacgaaat cggtcgggaa gctatgctgt
     4021 tattgcttga tcaaatgcag gggcaacacg ttggcagtgg ctctcgttta atggactgcg
     4081 aacttatcat ccggggatca acacgcgcgt taccttaaag taaaccttta agactgccgt
     4141 gactggtcaa aggcccgccg cttaagtaac atggcgggct gacgaacgaa taaatacagc
     4201 gaaacgatag tggcacaacg agattatgta cgccgcagcc aaccggcacc ttcgcggcga
     4261 aaaaagagca cctcacggaa aaagcaacga aatctgcctg cggtttctcc cgctatggtc
     4321 gctattgctg ccgccgtgct tgtgaccttt atcggtggtc tgtacttcat tacgcatcac
     4381 aagaaagaag agtccgagac gctgcaaagc cagaaagtga ccggtaacgg actaccacca
     4441 aaaccagaag aacgctggcg ctacattaaa gagctggaaa gtcgccagcc gggtgtgcgt
     4501 gcgcccacag aaccttctgc cggtggtgaa gtgaaaacgc cggagcaact gacaccagaa
     4561 caacgtcagc ttcttgaaca aatgcaggct gatatgcgcc agcagccaac gcagctggtt
     4621 gaagttccgt ggaatgaaca gacgccggaa cagcgtcagc aaacgctaca acgccagcgt
     4681 caggcgcagc aattagcgga gcagcaacgc ctggctcagc agtcgcgtac gactgaacaa
     4741 agctggcagc agcagacgcg tacgtcgcaa gccgcgccgg tacaggcgca accgcgccag
     4801 tctaaaccgg cttctactca gcagccgtac caggatctgc tgcaaacccc tgcgcacacg
     4861 accgcgcagt cgaagccgca gcaagctgcg ccggttgctc gtgccgctga cgcgccaaaa
     4921 ccgacggcgg agaaaaaaga cgaacgccgc tggatggtgc agtgcggttc gttcagaggc
     4981 gcggaacagg cagagacggt acgtgctcag ctggcgttcg aaggatttga ctcgaaaatc
     5041 accaccaaca atggctggaa tcgtgtggtc attggcccgg tgaaaggcaa agagaacgca
     5101 gacagcaccc tcaatcggtt gaagatggcg ggtcatacaa actgcattcg gctcgccgcc
     5161 gggggttgaa accctcaaaa tcccccccat ctataattgc attatgcccc gtacttttgt
     5221 acggggtttg tactctgtat tcgtaaccaa ggggtcagct cgtgacaact atagtaagcg
     5281 tacgccgtaa cggccatgtg gtcatcgctg gtgatggtca ggccacgttg ggcaataccg
     5341 taatgaaagg caacgtgaaa aaggtccgcc gtctgtacaa cgacaaagtc atcgcgggct
     5401 ttgcgggcgg tactgcggat gcttttacgc tgttcgaact gtttgaacgt aaactggaaa
     5461 tgcatcaggg ccatctggtc aaagccgccg ttgagctggc aaaagactgg cgtaccgatc
     5521 gcatgctgcg caaacttgaa gcactgctgg cagtcgcgga tgaaactgca tcgcttatca
     5581 tcaccggtaa cggtgacgtg gtgcagccag aaaacgatct tattgctatc ggctccggcg
     5641 gcccttacgc ccaggctgcg gcgcgcgcgc tgttagaaaa cactgaactt agcgcccgtg
     5701 aaattgctga aaaggcgttg gatattgcag gcgacatttg catctatacc aaccatttcc
     5761 acaccatcga agaattaagc tacaaagcgt aaggatctcc catgtctgaa atgaccccac
     5821 gcgaaatcgt cagcgaactg gataagcaca tcatcggcca ggacaacgcc aagcgttctg
     5881 tggcgattgc tctgcgtaac cgctggcgtc gcatgcagct caacgaagag ctgcgccatg
     5941 aagtgacccc gaaaaatatc ctgatgatcg gcccgaccgg tgtcggtaaa actgaaatcg
     6001 cccgtcgtct ggctaagctg gcgaatgcgc cgttcatcaa agttgaagcg accaaattca
     6061 ccgaagtggg ctatgtcggt aaggaagtgg attctattat tcgcgatctg accgatgccg
     6121 ccgtgaaaat ggtacgcgtc caggctatcg agaaaaaccg ttatcgcgct gaagaactgg
     6181 cagaagaacg tattctcgac gtgctgatcc cacctgctaa aaacaactgg ggacagaccg
     6241 aacagcagca ggaaccgtcc gctgctcgtc aggcattccg caaaaaactg cgtgaaggcc
     6301 agcttgatga caaagaaatc gagatcgatc ttgccgcagc accgatgggc gttgaaatta
     6361 tggctcctcc aggcatggaa gagatgacca gccagctgca gtccatgttc cagaacctgg
     6421 gcggccagaa gcaaaaagcg cgtaagctga aaatcaaaga cgccatgaag ctgctgattg
     6481 aagaagaagc ggcgaaactg gtgaacccgg aagagctgaa gcaagacgct atcgacgctg
     6541 ttgagcagca cgggatcgtg tttatcgacg aaatcgacaa aatctgtaag cgcggcgagt
     6601 cttccggtcc ggatgtttct cgtgaaggcg ttcagcgtga cctgctgccg ctggtagaag
     6661 gttgcaccgt ttccaccaaa cacgggatgg tcaaaactga ccacattctg tttatcgctt
     6721 ctggcgcgtt ccagattgcg aaaccgtctg acctgatccc ggaactgcaa ggtcggctgc
     6781 caatccgcgt tgaactgcag gcactgacca ccagcgactt cgagcgtatt ctgaccgagc
     6841 cgaatgcctc tatcaccgtg cagtacaaag cactgatggc gactgaaggc gtaaatatcg
     6901 agtttaccga ctccggtatt aagcgcatcg cggaagcggc atggcaggtg aacgaatcta
     6961 ccgaaaacat cggtgctcgt cgtttacaca ctgttctgga gcgtttaatg gaagagattt
     7021 cctacgacgc cagcgattta agcggtcaaa atatcactat tgacgcagat tatgtgagca
     7081 aacatctgga tgcgttggtg gcagatgaag atctgagccg ttttatccta taatcgcgtt
     7141 caatcatttt catcattgtt tgatggggct gaaaggcccc atttttattg gcgcgtatta
     7201 tgactgaaca acaaattagc cgaactcagg cgtggctgga aagtttacga cctaaaaccc
     7261 tccccctcgc ctttgctgca attatcgtcg ggacggcgct ggcatggtgg caaggtcact
     7321 tcgatccgct ggtcgccctg ctggtactaa ttaccgccgg gctattacag atcctttcta
     7381 acctcgccaa tgattacggc gatgcggtaa aaggcagcga taaacctgac cgcattgggc
     7441 cgctacgcgg catgcaaaaa ggggtcatta cccagcaaga gatgaaacgg gcgctcatta
     7501 ttaccgtcgt gctcatctgt ctctccgggc tggcactggt tgcagtggca tgccatacgc
     7561 tggccgattt tgtcggtttc ctgattcttg gcgggttgtc gatcattgcc gctatcacct
     7621 acaccgtggg caatcgtcct tatggttata tcggtctggg tgatatttcc gtactggttt
     7681 tctttggctg gttgagcgtc atggggagct ggtatttaca ggctcataca ttgattccgg
     7741 cactgatcct tccggcgacc gcctgtggcc tgctggcaac ggcagtactg aacattaata
     7801 acctgcgtga tatcaatagc gaccgcgaaa atggcaaaaa cacgctggtg gtgcgcttag
     7861 gtgaagtgaa cgcgcgtcgt tatcatgcct gcctgctgat gggctctctg gtatgtctgg
     7921 cgctgtttaa tctcttttcg ctgcatagcc tgtggggctg gctgttcctg ctggcggcac
     7981 cattactggt gaagcaagcc cgttatgtga tgcgggaaat ggacccggtg gcgatgcgac
     8041 caatgctgga acgtactgtc aagggagcgt tactgactaa cctgctgttt gttttaggga
     8101 tattcctaag ccagtgggca gcataactga caaatatcaa ttaacaattg atgattttgc
     8161 caacagccta catagcgcga tatactgaaa attctcgcag caactgaatg ttaagcctat
     8221 gaaatacgat acttccgagc tttgtgacat ctatcaagaa gatgttaacg tcgtggaacc
     8281 gctgttctcc aactttggcg gacgggcgtc gtttggcgga caaataatca cggtaaaatg
     8341 tttcgaggac aacgggttgc tgtacgatct gctcgaacag aatggccgtg gtcgtgttct
     8401 tgtcgtcgat ggcggtggtt ctgttcgtcg cgcactggtc gatgctgaac tggcgcgtct
     8461 ggcagtacaa aatgaatggg aaggtctggt tatttacggc gcggtgcgtc aggtagatga
     8521 cctagaagag ttggatatcg gcatccaggc gatggcggca attccggttg gtgccgctgg
     8581 cgaaggcatt ggcgaaagcg atgtccgcgt caattttggc ggtgtaacct tcttctccgg
     8641 cgaccatctt tatgccgaca ataccgggat tattctttca gaagatccgc tggatattga
     8701 atgataagaa aggcaccgca aggtgccttt tttctgcgtt acctgttggc ctacacagta
     8761 aagaaattac gcgaaagatg aagcgtaatc agacctcttc catgcgaccc agcagggcct
     8821 gcagacgttc ctgccagccg ttctgctgtt ctttcagatg gttgttctca cgctccagct
     8881 cttcgcgctg atgctgggca ttttgaactt cctgcgacag tgagttgttt ttttctttca
     8941 gctcttcgat ttccatctgc aacagagtga tggtatcaat cgcctgctgt acttttgctt
     9001 ccagtttctc aaacacttct aatgacattg tcatacctct cctgaattgc aaggcgttga
     9061 tggataaaaa atcctcgtcc cgattaccgg tgacgcctta ataaatacga gcgcacttta
     9121 gttagctccg attgtatgaa gccgcgccat cgctgtccag cggcacgcct tgcagattac
     9181 ggtttgccac acttttcatc cttctcctgg tgacataatc cacatcaatc gaaaatgtta
     9241 ataaatttgt tgcgcgaatg atctaacaaa catgcatcat gtacaatcag atggaataaa
     9301 ttgcgcgata acgctcattt tatgacgaag cacacacatt ttaagttcga tatttctcgt
     9361 ttttgctcgt taacgataag tttacagcat gcctacaagc atcgtggagg tccgtgactt
     9421 tcacgcatac aacaaacatt aactcttcag gatccgatta tgagtcaaac atcaaccttg
     9481 aaaggccagt gcattgctga attcctcggt accgggttgt tgattttttt cggtgtgggt
     9541 tgcgttgcag cactaaaagt cgctggtgcg tcttttggtc agtgggaaat cagtgtcatt
     9601 tggggactgg gggtggcaat ggccatctac ctgaccgcag gggtttccgg cgcgcatctt
     9661 aatcccgctg ttaccattgc attgtggctg tttgcctgtt tcgacaagcg caaagttatt
     9721 ccttttatcg tttcacaagt tgccggcgct ttctgtgctg cggctttagt ttacgggctt
     9781 tactacaatt tatttttcga cttcgagcag actcatcaca ttgttcgcgg cagtgttgaa
     9841 agtgttgatc tggcaggcac tttctctact taccctaatc ctcatatcaa ttttgtgcag
     9901 gctttcgcag ttgagatggt gattaccgct attctgatgg ggctgatcct ggcgttaacg
     9961 gacgatggca acggtgtacc acgcggccct ttggctccct tgctgattgg tctactgatt
    10021 gcggtcattg gcgcatctat gggcccattg acgggttttg ccatgaaccc agcgcgtgac
    10081 ttcggtccga aagtctttgc ctggctggcg ggctggggca atgtcgcctt taccggcggc
    10141 agagacattc cttacttcct ggtgccgctt tttggcccta tcgttggcgc gattgtaggt
    10201 gcatttgcct accgcaaact gattggtcgc catttgcctt gcgatatctg tgttgtggaa
    10261 gaaaaggaga ccacaactcc ttcagaacaa aaagcttcgc tgtaatatga ctacgggaca
    10321 attaaacatg actgaaaaaa aatatatcgt tgcgctcgac cagggcacca ccagctcccg
    10381 cgcggtcgta atggatcacg atgccaatat cattagcgtg tcgcagcgcg aatttgagca
    10441 aatctaccca aaaccaggct gggtagaaca cgacccaatg gaaatctggg ccacccaaag
    10501 ctccacgctg gtagaagtgc tggcgaaagc cgatatcagt tccgatcaaa ttgcagctat
    10561 cggtattacg aaccagcgtg aaaccactat tgtctgggaa aaagaaaccg gcaagcctat
    10621 ctataacgcc attgtctggc agtgccgtcg taccgcagaa atctgcgagc atttaaaacg
    10681 tgacggttta gaagattata tccgcagcaa taccggtctg gtgattgacc cgtacttttc
    10741 tggcaccaaa gtgaagtgga tcctcgacca tgtggaaggc tctcgcgagc gtgcgcgtcg
    10801 tggtgaattg ctgtttggta cggttgatac gtggcttatc tggaaaatga ctcagggccg
    10861 tgtccatgtg accgattgca ccaacgcctc tcgtaccatg ttgttcaaca tccataccct
    10921 ggactgggac gacaaaatgc tggaagtgct ggatattccg cgcgagatgc tgccagaagt
    10981 gcgtcgttct tccgaagtgt acggtcagac taacattggc ggcaaaggcg gcacgcgtat
    11041 tccaatctcc gggatcgccg gtgaccagca ggccgcgctg tttggtcagt tgtgcgtgaa
    11101 agaagggatg gcgaagaaca cctatggcac tggctgcttt atgctgatga acaccggcga
    11161 gaaagcggtg aaatcagaaa acggcctgct gaccaccatc gcctgcggcc cgactggcga
    11221 agtgaactat gcgctggaag gtgcggtgtt tatggcaggc gcatccattc agtggctgcg
    11281 cgatgagatg aagttgatta acgacgccta cgattccgaa tatttcgcca ccaaagtgca
    11341 aaacaccaat ggtgtgtatg tggttccggc ctttaccggg ctgggtgcgc cgtactggga
    11401 cccgtatgcg cgcggggcga ttttcggtct gactcgtggg gtgaacgcta accacattat
    11461 acgcgcgacg ctggagtcta ttgcttatca gacgcgtgac gtgctggaag cgatgcaggc
    11521 cgactctggt atccgtctgc acgccctgcg cgtggatggt ggcgcagtag caaacaattt
    11581 cctgatgcag ttccagtccg atattctcgg cacccgcgtt gagcgcccgg aagttcgcga
    11641 agtgactgca ttgggcgcgg cctatcttgc tggtctggcg gtcggtttct ggcagaacct
    11701 cgacgagctg caagagaaag cggtgattga gcgcgagttc cgtccaggca tcgaaaccac
    11761 tgagcgtaat taccgttacg caggctggaa aaaagcggtt aaacgcgcga tggcgtggga
    11821 agaacacgac gtaatgtaaa tgccggatga agcgtttatg ccgcatccgg tagtcccgaa
    11881 acgtgcgggg gcaaccccgc acacatcaat aatccctccc ttcccctgtg ctacacttcg
    11941 cgccattcct tactgcttag agtttgctat gagacgagaa cttgccatcg aattttcccg
    12001 cgtcaccgaa tctgcggcgc tggctggcta caaatggtta ggacgcggcg ataaaaacac
    12061 cgcggacggc gcggcggtaa acgccatgcg tattatgctc aaccaggtca atattgacgg
    12121 caccatcgtc attggtgaag gtgaaatcga cgaagcaccg atgctctaca ttggtgaaaa
    12181 agtcggtact ggtcgcggcg acgcggtaga tattgctgtt gatccgattg aaggcacgcg
    12241 catgacggcg atgggccagg ctaacgcgct ggcggtgctg gcagtaggcg ataaaggctg
    12301 cttcctcaat gcgccggata tgtatatgga gaagctgatc gtcggaccgg gagccaaagg
    12361 caccattgat ttgaacctgc cgctggcgga taacctgcgc aatgttgcgg cggcgctcgg
    12421 caaaccgttg agcgaactga cggtaacaat tctggctaaa ccacgccacg atgccgttat
    12481 cgctgaaatg caacaactcg gcgtacgcgt atttgctatt ccggatggcg acgttgcggc
    12541 ctcaattctc acctgtatgc cagacagcga agttgacgtg ctgtacggta ttggtggcgc
    12601 gccggaaggc gtagtttctg cggcggtgat ccgcgcatta gatggcgaca tgaacggtcg
    12661 tctgctggcg cgtcatgacg tcaaaggcga caacgaagag aatcgtcgca ttggcgagca
    12721 ggagctggca cgctgcaaag cgatgggcat cgaagccggt aaagtattgc gcctgggcga
    12781 tatggcgcgc agcgataacg tcatcttctc tgccaccggt attaccaaag gcgatctgct
    12841 ggaaggcatt agccgcaaag gcaatatcgc gactaccgaa acgctgctga tccgcggcaa
    12901 gtcacgcacc attcgccgca ttcagtctat ccactatctg gatcgcaagg acccggaaat
    12961 gcaggtgcac atcctctgat tgatttgatc gattgagcct tccagtcctt cgggactgga
    13021 atttttttgt tcggagaacg aagataaggc aagtcaatca aaacaggaga aaaacatggc
    13081 tgattgggta acaggcaaag tcactaaagt gcagaactgg accgacgccc tgtttagtct
    13141 caccgttcac gcccccgtgc ttccgtttac cgccgggcaa ttcaccaagc ttggccttga
    13201 aatcgacggc gaacgcgtcc agcgcgccta ctcctatgtt aactcgcccg ataatcccga
    13261 tctggagttt tacctggtca ccgtccccga tggcaaatta agcccacgac tggcggcact
    13321 gaaaccaggc gatgaagtgc aggtggttag cgaagcagct ggcttctttg ttctggagga
    13381 agtaccagat tgcgaaacgc tatggatgct ggcaaccggt acagcgattg gcccttattt
    13441 atcgattctg caactaggca aagatttaga tcgcttcaaa aatctggtcc tggttcacgc
    13501 cgcacgttat gccgccgact taagctattt gccactgatg caagaactgg aaaaacgcta
    13561 cgaaggcaaa ctgcgcattc agacggtggt cagtcgggaa acggcagcgg ggtcgctcac
    13621 cggacggata ccggcattaa ttgaaagtgg ggaactggaa agcgcgattg gcctgccgat
    13681 gaataaagaa accagccatg tgatgctgtg cggcaatcca cagatggtgc gcgatacaca
    13741 acagttgctg aaagagaccc ggcagatgac gaaacattta cgtcgccgac cgggccatat
    13801 gacagcggag cattactggt aagcggttac ttatcgataa acggcacgat gagcaaatcc
    13861 gcactcatct tattgatcat cccgcgatat gccggcatca aacggttgat aaatgagtgg
    13921 tgatgaccac agacaaggag gtcgcactgc tctttttgca taatttccag cagtgtttcc
    13981 ggcatttctc cgcgttcaat acgcagtttt gtcttcggcc attgaatatt tttcgtcagt
    14041 ttatacagct tgttatccga cttattcttc aacaattgaa gaatatcttc tgttgcaggg
    14101 aagtagatac ccggatacaa ctcgcttaag ccatcatcaa tatgaattaa cgtcaggtga
    14161 gcgtcattat gtctggcgag ctccagggct ttattcacca gtaaggcatc ttcttcattc
    14221 ccggaaattg ccacgccaat gtgtttataa gccatgttta actccttcta aagcaactcc
    14281 atcaagctaa cgaacgcagt gataactcaa aaataatcat ctcggcctgg catgagaaag
    14341 tgaaggccgc atcaagctca acttgcccct ccagatcctt aatcttatag gccacgctgg
    14401 ccccttcatc tgcagaggat gcagcggcgg caagctcttt cagtttggtc ataaaagact
    14461 ctgcttcttc gcgggtggca aaaaagcgcg aaaacttact ggtacagttg tcgttatcaa
    14521 tcaccgtacc gatatcaatc gcacatcctt tagtactgca tttatctacg acatctttca
    14581 tagggcacct ctgtatattc gcctctctgt cgattacgac ttaagaataa tgcgccgtta
    14641 agggaagaac aaacaagtaa tgatcttatg gattaccatt ttttttgata ttgcttaagg
    14701 tgaagagttc agaaggattt tccgggtatt gtcatttgca tcgtggaaat aacaatcccg
    14761 gaaaaaaata gcagtaagag atatattaaa ggttaattac ttgatttatt gtcggcttta
    14821 tacttcacat cctgagtatc tttaccatat ttattttcgc cttgtgtgcc aacaaacgca
    14881 ccaagatcga taagcatcat caccagaatc aacgtcggga caaaacgccc caccgcccat
    14941 tgccagacac ccggtaaaat cgcccagtta cccgccagca gcatccacgc cacaatcatc
    15001 agaaatgccc atgcgccgga acgcccgcga tcatgcaagc gcttaacagt tactgccgct
    15061 gttggccaaa gcaagcacac aaggcaaaac gccgcggtct gaatatcgag taaattttta
    15121 cccgccagtg agaaaagcac cagcatgcct gcgaaccaca ggcctatcca aatccagaaa
    15181 tcacggcgtc caatacgccc tttaaatgag aataaccatt gctgtatggt catgtaagtt
    15241 ccttgatggt tgtcttttcc aggattctac ccttttgaca agggcgacag atatcgtttt
    15301 aatcggagcc agtcataaca aaaggtactg tcaatgaagc cagggtgtac gctgtttttt
    15361 ctcttatgtt ctgcattaac cgttacaaca acggcgcatg cacaaacacc agatacggca
    15421 acgaccgcgc cttatctgct ggctggagcc cctactttcg atctctccat cagccagttt
    15481 cgagaagact ttaacagcca gaatcctagc ctgccactga acgaatttcg tgccatcgac
    15541 agcagtcccg acaaagccaa tctcactcgt gctgcaagta aaattaacga gaacttgtat
    15601 gcttctacag cgctggagcg cggtacctta aaaatcaaaa gcattcaaat gacctggcta
    15661 cccatccagg ggccagagca aaaagcagcg aaagcgaaag ctcaggaata catggcagca
    15721 gtgatccgca cactcacccc attaatgacc aaaacacaaa gccagaaaaa actgcagtcg
    15781 ctactaacgg cggggaaaaa caaacgttat tacaccgaga cagaaggtgc actgcgttat
    15841 gttgtcgcgg acaacggcga aaaggggctg accttcgctg ttgaaccgat taagctggcg
    15901 ctatctgaat cgcttgaagg tttgaataaa tgacaaaaag caaagccttt gtgccgatga
    15961 atctctatac tgtttcacag acctgctgcc ctgcggggcg gccatcttcc tttattcgct
    16021 tataagcgtg gagaattaaa atgcgacatc ctttagtgat gggtaactgg aaactgaacg
    16081 gcagccgcca catggttcac gagctggttt ctaacctgcg taaagagctg gcaggtgttg
    16141 ctggctgtgc ggttgcaatc gcaccaccgg aaatgtacat cgatatggcg aagcgcgaag
    16201 ctgaaggcag ccacatcatg ctgggtgcgc aaaacgtgga cctgaacctg tccggcgcat
    16261 tcaccggtga aacttctgcc gctatgctga aagacatcgg cgctcagtac atcatcatcg
    16321 gccactctga acgtcgtact taccacaaag agtctgacga actgatcgcg aaaaaattcg
    16381 cggtgctgaa agagcagggc ctgactccgg ttctgtgcat cggtgaaacc gaagctgaaa
    16441 acgaagcggg caaaactgaa gaagtttgcg cacgtcagat cgacgcggta ctgaaaactc
    16501 agggtgctgc ggcattcgaa ggtgcggtta tcgcttacga acctgtatgg gcaatcggta
    16561 ctggcaaatc tgcaactccg gctcaggcac aggctgttca caaattcatc cgtgaccaca
    16621 tcgctaaagt tgacgctaac atcgctgaac aagtgatcat tcagtacggc ggctctgtaa
    16681 acgcgtctaa cgctgcagaa ctgtttgctc agccagatat cgacggcgcg ctggttggcg
    16741 gtgcttctct gaaagctgac gctttcgcag tgatcgttaa agctgcagaa gcggctaaac
    16801 aggcttaagt ctgacaggtg ccggatttca tatccggcac ttactttcct taactcttcg
    16861 ccttaacgca aaatctcaca ctgatgatcc tgaatttcct cggctgaagc acggttaagc
    16921 gtcagtagat ttcgttgtgt cgccagcaat acaaatgagt tatcactctg ccgtaccatc
    16981 gccagcccgt agcttcccat atgttcccgc gcctcaggta cttcttctgc cagcatcata
    17041 aatgggctgc gttgtaccag ttcgctttcc gttacccgac gcgccaggta ttcatgcccg
    17101 cgcaaaccac ctggcagtgg caaccagcgg ctgctgatgt tcgccagatt gttatccagt
    17161 tgttcgcgca catcaggacg aatacaagag atatgaatat gaaaatggtt ttgcgtacgc
    17221 ccggtgcggg agttgatcgc caaagaaacc gcgcgatcgg gaaccggctg gccgtatttt
    17281 ttgctcataa aatcacgcgc ctgccaggcc aaccaaaaga agttcggcgt tgaaggatcg
    17341 gtcaacaaag gactttcagt accgttaata cgatacgttg gcatcaacag atattgcagt
    17401 ggaccattaa gatcttttaa aaccacgtat ccggcattgg gtttgacttc cgcacatggc
    17461 gaaggatttt gattttgctg ctgattgggc aaacattcct caaggacaat cttacgtaat
    17521 gtatccgact cttcaccggt taatttccag taaccaatac cggcagccac aacggcgata
    17581 actatcatca ccaaaaaaag aagacccgct tttttcatct ttttttccct gtacctcaaa
    17641 gagatgcaag ggtaacgcaa aatcgtgaca aataaaaaac ccggtcgcga gaaaccaccg
    17701 ggtttgataa ttatcctggg gaaatcagcg tttgctgatc tgatcgaacg taccgccgtt
    17761 agcaaaatgc tctttttgcg ctttcgtcca gccgccgaac tcttcatcaa tggtgaataa
    17821 cttcagcttt ggaaacgcat tttcgtactt tttcgctact tcagcgtcgc gcggacggta
    17881 gtagtttttc gcggcaattt cctgaccttc tggcgagtag agatatttca ggtaggcttc
    17941 cgccacctct ttggtgcctt ttttctcgac cactttatcg accaccgaca cggttggctc
    18001 tgcgaggata gactcactcg gcgtgacgat ttcgaattta tctttcccca gttcattcgc
    18061 tgccagcaga gcttcgtttt cccaggcgat cagtacatcg ccaatcccgc gctcgacaaa
    18121 ggtgttagtg gagccgcgcg cgccagaatc cagaacttcg acgtttttat acagtgcccg
    18181 aacaaaatcc tgtgcttttg cctgatcgtt gttgttgtga tgcagcgcgt agccccaggc
    18241 agccaggtag ttccagcgtg cgccaccaga gcttttcgga ttaggcgtga tcaccgaaac
    18301 acccggttta atcagatcgt tccagtcatg gatctgcttc ggattgccct tacgcaccag
    18361 gaaaacaatg gtggaagtgt acggtgcgga gttatccggc agacgtttga tccactcttt
    18421 atcaatccgc ccgcgttccg caatcgcgtc cacgtcatag gccagagcca gcgtgacaac
    18481 atcagcttca ataccgttga ttaccgacgt cgcttgttta cctgaaccac cgtgtgactg
    18541 acgaatcacc acgttatcac cagtttgctg tttccagtgg gcgctgaatg ccttgttgta
    18601 ctgttcgtac aattcgcgcg ttggatcata tgaaacgtta agaagctgaa tatcctttgc
    18661 cataacgctg gttgccgcca gcaaaaatgt taaccctacg ccccacttgt tcatcgcccg
    18721 actctcttat gttgtgttgt gatgagcaaa gcgtgccaga aggttaacca aacattaaag
    18781 aataaaaaaa gattggctat aacttgcggg tatatgttga gggattaaaa aggcggaaga
    18841 aatccgcctc atattgctga caaagtgcgc tttgtccatg ccggatgcgg cgcgaacgcc
    18901 ttatccggcc tacaaaagtt tgcaaattca ataaattgca gaattcatgt aggcctgata
    18961 agcgaagcgc atcaggcatt tttgcttctg tcatcggttt caggctaaag gaatctgcct
    19021 ttttccgaaa tcattaatac agttttttcg cgcagtccag ccagtcacct ttgaacggac
    19081 gcttcatgtt ttcgatagcg tcgatgatgt cgtggtgaac cagctgttcg ttctggatac
    19141 ctacacaacg accgccgtaa cctgccagca gcagatcgat agcgtaagcg cccatacggg
    19201 aagccagaat acggtcgtaa ggcaccggag aaccaccgcg ctggatgtgg cccagcacag
    19261 ttgcgcgggt ttcacgaccg gtttctttct cgatgaaatg cgccagttcg tcaacatcac
    19321 acatatgttc ggtaatcgcc acgatcgcgt gttttttacc tttcgcgata cccgctttga
    19381 tttcgtttac caggtcttca cggctgaatt caacttccgg aaccacaacg aattcacagc
    19441 caccggcaat ggccgcagcc aacgtcagat cgccacaata acggcccatc acttccacca
    19501 cggaaatacg ctggtgagaa gaagaggtgt cacgcagacg gtcgatcgct tctacaacgg
    19561 tgctcagcgc agtgaagaaa ccgatagtgt agtcagtgcc tttgatgtcg ttgtcgatag
    19621 tgcccggcag accgatgcac gggaagccca tttcggtcag acgcattgca cccatgtagg
    19681 aaccgtcacc gccgataacc accagcgcgt cgataccacg ttttttcagg ttttcgatag
    19741 ccacggcgcg gatgttctcg tcgcggaatt ccgggaagcg cgcagaaccg aggaacgtac
    19801 cgccacggtt gatcatgtca gaaacgctgt aacggtctag ctgtaccata cggtcttcat
    19861 acagacccag atagccgtca taaatgccca ttacttccag accttctgtc agcgcagaac
    19921 gaacaacccc gcgaattgcg gcgttcatgc ctggcgcatc accgccgctt gtcaacacac
    19981 cgattttctt aatcatgact acctctgaac tttggaatgc aaaataaaat ctgttgccgg
    20041 aagtcttctt gcacatcgaa gtgatccaac gaatgtgcaa atagtataac aatcacttcc
    20101 tgctgaattg attcaggtca ggccaaatgg cggtatttta tacacaaaat gcgggtctgg
    20161 ctctctttta tactgattat gaaagcatag accgtttacc ctccctgggt acgacggaac
    20221 aggggtcctg atggataatt acatccgatc ccggaaaacg ccgtaaaata gcctgctcta
    20281 cctgatccgc caccatatgt gcctgaacca aaggcagaga gtcttccatt tccaaatgaa
    20341 tctgaataaa gcgggtcggc cctgactgcc gcgtgcgaag atcgtgagcg ccgctaacac
    20401 ccggccagga agtcacgata tcaataattt cttgccgttc ctcatcaggc aatgcgcgat
    20461 ccagtaatga ctgtaccgcc tcatatccca tgcgtaacgc gctatataaa atatagatgc
    20521 cgattcccaa tgcaaacaga gcatcggcgc gatgccagcc gtaccaggac aaccccagcg
    20581 ccagcagaat tgcgccgttc atcataacat cagactggta atgtagcata tcagcccgca
    20641 ccgcctggct ttgcgtccgg cgcaccaccc accgctgaaa cgagacaagg ataatcgtac
    20701 aaattagcgc cacaattgtc acgataaccc cgacgcctgg atctgtcatc ggtgttggag
    20761 ataccagatg ttgaataccc gtcaaaaaca ggaatagtgc cgaaccggag ataaacatac
    20821 tttgcgccag cgccgcgagg gactcagctt taccgtgacc aaacgagtga ttatcgtcgg
    20881 caggttgcag ggaatatcgc accaccagta aattcgtcaa cgacgcgccg atatccacca
    20941 gcgaatccac cagcgcggcg agaatactca ccgacccggt ataccaccat gcaaaaattt
    21001 taatcagcaa tagcagcgaa gccatcgccg tcgcagcaat cgccgcccga ctgaccagcc
    21061 gtccataaga ttgattcata aatactcccg ctatcaactg acgctagtat aacggaagca
    21121 aatcatctgc aatgcattaa gcagcaggca aattgaggat aaaaaaaccc ccacatcatg
    21181 tgggggaaga cagggatggt gtcacaaaaa agtacctaac ctgatgatat aaatggattt
    21241 atgtcaaccg ctgtccacat agcgtccaca tcgaccataa ataagcccct caactgaggg
    21301 gctatttttt tgatcacatc cacataattt gctgccctga cggcaacggg tgcggcctta
    21361 cggcgtggac ttctcccggc ttcacgatgt atcgctgtac cgactcataa gtgatgaacg
    21421 tggcgctgca attcacgttc tggcactggt gataacgctc ttttgtcgtg tcagtgatat
    21481 agcgacttgt acgcgcatgt gcggcatgct ggcataaagg acaatgaaac atcgcgagca
    21541 cctcttccgg ttttgttgat ggtgccattt tagttaattt atccttataa aacaaacaga
    21601 taaataaacc acgtcactca tcatcttctg tttcgtactc cacatcagaa agccttacct
    21661 caagctccaa ggacgtcgtg aagccgctat tattgagaca atgtgtcacc ttagtgatgg
    21721 tccagtcctg ctcgtctatg acgcgcttaa agcctgacac tttaaccggt gtttccgtgt
    21781 aaatatctgc acgaccggta gccaggctga tggagaactc cgctacaccc cgttgcagtt
    21841 tatcccactt cgcctgagcg gcgcgcatgg cctgcgcttt tgtggcatat accgtggtca
    21901 gggcaaaaac gttgtcagct tcaccggcca tgtattcacc ttcgcgcgct tccggtactt
    21961 ttggcgcttt cttctgcgtg accggtttcg ctttcgggtg ctccagtgcg cgcaggtgtt
    22021 tttctttctt tttgcgtttc agttttacct tctgcttttg cggcttcggg tctttggtgt
    22081 gtaaccactt tgccgttacg ccggtgtaag ctccacggtc agcaatcgca aaatgatgac
    22141 ggtcgccgtc gctgcgggtg atggtaatct gcgggatttt tttaccgctg gccgtcaccc
    22201 cctgccccgc tttgagaaac agcagttttc ccatttttac cgacacctcg ccgccgttgc
    22261 gttcagcaag acgggtcagg aatttcgcat cagactcctg cgactggtcg atgtgtggaa
    22321 tttttattcc ggccagtgac ggagcgacac tggcttccag cctgttacgg gaggctatcg
    22381 cctcaacaat cgcaccgagc gtaatgtcat gccatgagcc ttcacggcgg gaattgagcg
    22441 tcccgcgaaa atctgcactc cgggcgcgga tggtgaccac atccggcgcg ccccggtgtt
    22501 caacctcatc aacggtaaat ttccctttgc ataccagggc aaaacctttc cagccgatat
    22561 acaccgtcag gacagcgcca cgaaccggca gcccgacctg cccgtcggca tcgttcagtt
    22621 caatatcaag ctggtcagcc tcaaagcccc ggttatccgt cagggtcatg ctcatcagac
    22681 ggtcgctgat attgccggta atatccctgc tgtcgagcat cagcatgtaa tccggcgtca
    22741 gcgtactgcc tgcatcaaat gtcagcgcat ccagcattat cccgcccccg tcatacccgt
    22801 gaatttagtc gccatactgc cagccttacc gatgagcgat tccgcctgtt taccgatatc
    22861 gccataaagc gcggccagtg actcatcaac gcgggtgagc gacagcgtaa aatcaatttt
    22921 ccgtggtgtg ccgtctgcaa agaaaatact ccctgtttca ctcaccttgc tgatgacata
    22981 catgccgtaa atcatgccgg tgccatccag caacggccac gcccggcctt cctctgccat
    23041 cagtctgagc gtggtcatcg tcagctttcc gccagtcagt tcgggataaa gcacaccggc
    23101 aagtgtgatg ttctcctcac ctacaccgag aaactgatag gcatcccgtt taccgatacg
    23161 ggaatttgac ggccagcgat aatctgattc acgctgcatg gtctggtgtg gcagcgtctg
    23221 gcgcataaaa acaaacatac ctaacgcgag catcattttt cgtcacctcc ttaaccgtca
    23281 tgcatcatgc tggcacgggc gcgcgcacgt ttatcccgct cgtatttttc gagcgcatcc
    23341 tgtaactggc ggtcaagctg tgtccccggt gcagtaccac ccgtcaggct gatgtgatat
    23401 tcgtttttac tctggtccac ataagagcgg ccagccggtg ccgtgaccgg ctgataagcc
    23461 tgatagcctg cataagagct ggtcgccgga atataaccac cggtgccata cgtggcggca
    23521 tgagttctgg cggcggtctg gtcaagtgtg tctgactctt tgttgatgac cccgagcttt
    23581 tccagtaccc agtcaatgcc gctgcgcaat ttgttgaacg cattaagcgg cagcatcagc
    23641 gcgtcagcca gtgcctgccc gaacatgacg cccgtgtcac ggcaacggtt cagggtgtcc
    23701 tgggtggctt tgaccggggc aatcaggttt ttaaaccact gccacgcagc ctgtaacttt
    23761 tcgcccagcc agtcaaacac cggtttcagt ggcgtgaaca gctcccccac cggcgcaaat
    23821 gccgctttca gcccttcaac cacaccgcca aagaatgcgc tgacaggctc ccagtattta
    23881 cggataagca acgccccggc gacaatggcg gccaccacag ccacaaccgg ccagctaatc
    23941 gccccgatgg ccgtcataac ggcactacca accgtcgtga agattgcccc cattgcgcct
    24001 gctgccgcga tgatggcatt gatgccggtg ataaccggcc aggctacaag accaattgca
    24061 ccgatgatgc cagtcagcgc cagcgcgcca ccggcaatga ggccaatggt tgacgccagt
    24121 gatttgtttt tctgtaccca gccgtcgagt tttaacacat actttgtggc cgtctgcgtg
    24181 agcttacgta gcgcgccttc ctgctggtca aacaggtctg tccccaccgc ctcataagcg
    24241 gactgaaact ccttaaagtc accgccgagg ttgtcctgca tgatatttac cagctctgcg
    24301 gtcttcccgt ctgaggcttt aaacgcagcg gtcagtttgt ccagctttcc ggttgacgcg
    24361 gcagtcatca gcacagcggc ggctgagctg gcctcctccc cgaaaatggt tttcatgtat
    24421 tcggcctgct gggcagtacc gagccggttt ttctcaaagc tggcctgcat ttctttcaga
    24481 atggtaaata ctggtcgggt gtttccctta ctgtctgagg ttttcacgcc aagctctttg
    24541 agtgcatccc atgcttttcc agtcggtgcc tgcaggcgac ttaacacggc acggcttccc
    24601 gtccccgcca ttgagcctgt gatttttgca tcatgcagcg ccccgaccat tgcggcggtt
    24661 tcttcaatgc tgacaccggc attttttgcc actggtgcgg cataggtcag cgcatcgctc
    24721 atgccgtcaa aatcagcggc ggttttgttc atcgtcatgg agagaacatc cccgatatga
    24781 gcgaccttat cgtttgaaag ctgaaaggcg gatttcatcc ccatcagcag ggcggcgttt
    24841 tcttccattg tgcggcggtt cgccagcgcc atattcagcg tgaccggcgt tgccgcctga
    24901 atggcatcaa catccccacc cgctttcgca atgataatct gtgcaccggc cgcatcatcc
    24961 gctgaggcgg cggtgttgtc accgagctgg cgcgcctgct tgcggagtgc agccatttcg
    25021 gcggagtctt ttgccacacc gagcacagcc tgcaattctg agtttttctg cgcaaactca
    25081 taacctggca tcagcagctt aactccggct atcgttcccg cagcagcaat ccccacaccg
    25141 gcagcgccca ctgaggccat atttccggcc agttcctttc cggcctgata acgctgtttg
    25201 actgcgttaa gttttgcctg ttgcgcactg acacgcgcca gcgcgtcacg ctgccggtta
    25261 agctgggcgg tggtttcact gatacggttt ttcagtccct gctcatcatg tgcaagattg
    25321 cgggtattaa ttcccacagc ggccagttcc cgctgctggc gtttaacgga atctgtcagg
    25381 cggttatatt tcgcctgtaa gtcctccgcc gcacgctttg cggattccag cactttcgcc
    25441 tgagcacggg tcgggcgttc ggtgttttta aactgtgtgg caagggcttc ggcctcctgc
    25501 cgtgcctttt caagtgcatg accagtcacg gcgagctgtg cactggtctt gcggaatccc
    25561 tcaatacggg atgcgtgacc gttcagctcg cgcagtgatt tttgtgtttc ccggatatcc
    25621 cccgacagca acttactcgc tctgcggatg gatttaaacg ggcgggacgc ctggtcaaca
    25681 gccctgagca atacctgtaa ttttacattg ttactcattc gtgtttccgc ttcgcctgag
    25741 cgccttttcg cgccatgtga tgagttcggt caggctcatg ggatacagtt ctgatggcgg
    25801 ccagtgaaat atcactgcca catccgccat caggtcatcg accgacagat ttttcggaag
    25861 cgtcactgca ccgagttcgg cgacaaaaaa ccgaccacct taccggccag cgccacaagg
    25921 tcaggcagtt ccagcgcggc gacttcctgc tcggtcagca tcggtgccgt catgcgcggc
    25981 agcaccttaa tcagtgcatc gacttctgag tttgcgaccg cagccagact gacaccgcgc
    26041 agcgtccccg cactgggttt catcagcgtg acctgttcga taacctgctc accacgcttg
    26101 accggattgt ccagggtaat cacattttct ttgttcatgg ttttctcact tctgaatcgg
    26161 ggttaaccgg tcagccaggc tgaccggatg aaaattacag gccgatattg cggcggtgtt
    26221 gctccagccg gtcgacgccg ttcaccttct caatcatgtt gatggtgtcg atttcgacca
    26281 gctccttacc gtccatcgtc agccggaaat aggtgcagac cacggagatt ttcgactcgg
    26341 tgtcttctcc ctgtttaccc tcgccggtgt cgatttcttt ctgacggcca cgcatgacca
    26401 cctctacggc caccgtttcg ccggtatcgt cacgctggta agagcctgca aaacgaatcg
    26461 gcacggcatc cacaccggtt gcggcgtaaa gctcccagat aaccgaatcc gggaagcccc
    26521 cgagcgacca ctccattgac agcgcatcgt catcaaggcc gaggtctacc ggtgcgcttc
    26581 cgttcatccc cgcaccgcga tagttttcga gtttacgggt cagttttggc agcgtgacgg
    26641 actttgcaac gccctgatag ctgtagccgt tcagaaagac gttcattaac ttgagtttgc
    26701 gcggcattgc catcggtcag gctccttaat tgctgttaac cgaggtgacc agatttgcca
    26761 ggtatttatc ggtaatacgc tggcgcaggg tcaggttttc aagaggaggc accggggtat
    26821 agtcgtagtc gatatacagt tttccggcct tgagggtttc cgcatcgttg gattcttcgc
    26881 tgaaccagca ggtcgcatcc acgatatagc cgtttgtttt cagctcacgg aatttggcat
    26941 tgatgccgtc aacgatgtcg cgaatcagcg ttgcggtgat gggcttgtcc accgcccaca
    27001 tgtgcgcctc agccatcgtg tcggccagca cctgcgcggt gcgggtgtag ttttcaaaga
    27061 ggaacagcgg gtcatcagag caggtacggt taccccagaa gcggaaaccg tcacggcgaa
    27121 tcagcgttgt gacgcctgac tcgttaagca ggtcagcatc ggtgccggac tcctgcaaat
    27181 cccagaatac ggacgcgctg atgccggtaa caccgttcac cccgacgttg gacagcgttt
    27241 tatgccagcc ctgctcctgg tcgattttag cgcgcagacc cagcgcacgg gcggtggcat
    27301 acgcggtggc ggtggtactg gcgaccgtat cccatgcgag gaaatccggc cagatgacca
    27361 tcagctcacg ctggctgaaa ttctgacggt aggctttcac ctcggaaacg gtcttacagc
    27421 cccatgcgct gatatacccg aaagcgcgca gcttctgaca gactgatgcc agtgcaacag
    27481 ccacctcttt ggtgtccagt cccggcacgc cgagaatacg cggtttaaca ccggttaccg
    27541 actccgccgc cagcagggct ttcagtccgg tgtactgacc gttttcgtcg gtggtgccga
    27601 tgatattgga aacggtctgc gcgagtttcg tttcctcgtc gtcgccggtg ccgtcttcca
    27661 cacgcacgac aacggtgacc ggttttgact ggtcggcgat ggcctgcaac gatgccgcca
    27721 gcgtgccttt tttaccggcc tttgcaattg cgctctgcac attggtaatc agcaccggtt
    27781 tattgagggg gaaggtttcc gcatccgcat cgctggccgt gcagaccatg ccgacaatgg
    27841 cagtggatac ggtggaaatg acgcgggtgc cgtcgttaat ctccagcacc tgcacgccgt
    27901 gatgatagtc actcatccgt ttaactccgt ggttaatggg tgcaactatt ttctgttggg
    27961 cagtgcatga gacgctattt gacctggctg gtcagtggat gaaacaacag ataaagaaaa
    28021 tgcgggcaat tcgcccgcat gtcctgattt gtacacactc attttccgac tgacagttta
    28081 catagcccaa acgctatcaa atctgacagc ctgctatgag cgaaaagcgt taaatctcac
    28141 gatatctgct accgggaaaa atttcgatta tcttcgatgg gaactttgat aatgtagtaa
    28201 atgccatttt cagcgatcac ttttcaagcg tgtgtaaatg attaatgtta aaaaattgac
    28261 ttcactgaaa taaggttgct agtatttttg gagcaaccat taatgttttt tatttaagga
    28321 atgtaaatga catacgcact gattgataat tcaactctaa ctgcagtcca aagaatcgag
    28381 gggaaagtaa aaacaagatc gaaggactca gtagatacag acataatagc atttgagaac
    28441 tatatacagt ctattttgtt ctatgatcac gttatcgcgg ttgatgatta tattccagag
    28501 taccgtggga gtaggatttc aagtttcaga gagatattat ttcttgataa agcacaatat
    28561 gggttggatg aaattgaaag tgaagcaaaa aaaatatctg atgcattgaa acctaaaatt
    28621 cgtggtggaa catttgttaa tgacgatttt aaaaaactaa ttgaacttct tcagacacat
    28681 atcatatgta catgggacat tagttcaagt gtttatcatc taacactgaa gaacttatct
    28741 gatggaggta aggagtttga aaaatatggt aacattgctg ctgccatttt ttcagaactg
    28801 cgtgatgctg ctgagtctgg taatagaaca aatggcgatg ttgaacttct cgatcgattt
    28861 ggcaacccta ttcgcaaggg gtacaaagtt cccggagcaa aatgggggga tggcacatct
    28921 gctggtgaag catcagatgc aatcaaagca ttcgtggctt cgcttgtatg gctggctaat
    28981 cgggcagtat tttattctct cacagctaag taccttcaag cagatacttt tttatatcca
    29041 ttacgtcagg cgtatcaaca gacatattta agccaaaact gtcagtatgg ttttgactat
    29101 gctaaacata ttgtagaaaa cttttccact tcgttaagtc atgatctcat tgatattcat
    29161 tctggtggta aagctgtagc aacagcaact tcgcttcctt tattttcggc atggctcgca
    29221 aaagaaactg gtgatccaag ttgtattgtt gaagctgctt ataacataag ggacaacaac
    29281 gaatttgtaa gggcccgtga gaaaatgcga gaaataaagc gcttgtttga tgaaaatgaa
    29341 ttgcctcaag ctaataagtc agtaagagca ttgcttactg aagtatcgaa agaatcagat
    29401 tatattcgaa ctaagtatgg gctcaagact agacaaggtg tacctgtaac taagcttgta
    29461 catgtataca atactgttgc ggctattaat tcccttccga aactaccgac ctttaatttt
    29521 aaagttaaag ttcctgattt cttatatgat cttaaaaaac ccaaggggtt tcgtgctctc
    29581 tatcgaaatt tgactaatga tctttctact gtgtggtcgt taggagaagc tcgagatatt
    29641 cttggaagta aagttgtgat agataatcat gcaagaattt acagtccgaa gcatgaagaa
    29701 ccgcgtttca aaaactctca ttccccatat aagagcccga tgtagcttaa gaaaaagaca
    29761 cgcccacctt gtgcgtctac ttctggcaca gagcggactc tcagattagg ctttactctg
    29821 tgccatagat atgtaagctc acaccagagc tcatacaact tattgcggca tttccggcca
    29881 ttcaggattt gcaggatcca cacgactgac cagaacactg tagcgttccc atgcctccag
    29941 tcgactgcgc tcctcatctg ttgccatatt cagcctgaca gcgcgctcca gcggcaaaat
    30001 cacggattca gcatctgcaa gaagtctggc tttccgattt tctgcctgct gctgcaattc
    30061 ctctgccgta taaattcgtt taatcactgt acagtcctta aacatccagt tccctgaaat
    30121 gtccgcccgt cggttagcag taatatccgg cacttcaaca acacttaatc catccggtct
    30181 gatagctgtc acatcctttt ccacatagcg gataatatta tctttgtcgt acgctatttt
    30241 tatcgtgtca tcagcaaaat acttttgttc ttcgtaccag ttcttaccat cttctgaaaa
    30301 aaaccagaca acatcaaagt cctttgtcaa ttgatattgt tcaaccgttt ttggattacc
    30361 tgccgttata tttatcaaat gctgcataaa ttatacctgt gccacgttat accaagtccc
    30421 gttaatgtat ttctgcaccg gtctgtaata tacgccacca atgttatcgg cagagtttga
    30481 gccggtatcc tgaacaataa tgccggaata tacacacccg gacggtgcct gatgtgtcca
    30541 tgtcatacca ttgttcgcag gtttgtatgt ggcagcacca ccaagccgga tatcccggac
    30601 atagcgtgaa tcaaagttgc catagttaga tggtgatacc tgcccgttaa cagcaaaagt
    30661 gatgctgtta tctgtatttc tctgactgta aaaatgccag cctgcatcat cacctaattc
    30721 agccaccaca ggacgacttg agtttcccca caaattgaat gcggcttcct tcgtggatgt
    30781 attgctgctg ctgaccgtga actttttccc gctaccggca cgtactttgg tacttgagac
    30841 aatatcacct gtaacactca ggccatgccc cattgacact ccgccattgg cgttattgat
    30901 agtcagcggt cttaagccgt tccatgtccc aaatttatcg ccagaggccg tcagcattaa
    30961 atatgtgctg ccgccatcat tcctgataaa gaatccataa ttgccataag caatgcgcag
    31021 accattagca ctgagtgatg taatttcacc tcttgaacgg agaccataag cggagctgag
    31081 tgataattct tcctgagcgt cattgtttcc tgtcgcccag cgaattaccc cgccctgaac
    31141 tgtttcatgc cagatagtat ctccttctcc accacgaaac tttctgagat attttttgcc
    31201 gcctctggtg cctgaacata aggccgtaga catataggca ttctggcttc cgccatcctg
    31261 attaatcgtt ccggtcattg agtcgccctg acgattccag tcacgacgcc agccggggga
    31321 gtagccgtcc ccatgattta tgtaagtgaa ttgcgcgctg gttgtaccgc caccgcttga
    31381 tgttgttggc gtggtcactc ggatagtgat tgcagattta gttcccatga cctcgacgac
    31441 acaaccagcc aggtggatat caccacatcc ggtatccgta atgattttgt tatttgcata
    31501 tgaccaggag cctttgcaca tccagtacgg atgattaaat gcaccacggg aatccagcca
    31561 ttcaataaac tgagcggttg tccagtttcc ggcttcagtg ctcaaagcgc cgctataagc
    31621 acgacaggca ccgatatttt tcgtgaaggt atcctttccc ggaatatccg caccgttctg
    31681 atctttctga agacgttttt cagcattgtc ataggcagac ttcactgctt ttggcgttgc
    31741 ggccagcgtt tcagaatcgc tgttgatggc actacttagc tggacaagac cttttcgcgc
    31801 tgtggtggca tcctgtgcag tgtatttccc gttagcaagg tcatacgctg tcttaaccgc
    31861 ctttggtgtt gccgcaagcg tttcagaatc gctgttggtg gcgctactga gctggacaag
    31921 accttttcgc gctgtagtgg cgtcctgtgc agtgtatttc ccgttagcaa ggtcatacgc
    31981 tgtcttaacc gcctttggcg ttgccgcaag cgtttcagac gtgctgttgg tggcactact
    32041 gagctggaca agaccttttc gagctgtggt agcgtcctgt gcggtatatt tcccgttagc
    32101 aaggtcatag gcggccttta ccgctttcgg cgttgcggcc agtgtttcag acgtgctgtt
    32161 ggtcgcactg cttaactgag taaaaccttt tgcggtcagc gaggcgtccg ggtgacgtcg
    32221 tgactgttcg tgctctgcaa ttttgtcatc aacgtaatcc tgtgtcgcca tcaccgttgt
    32281 ggtgtcaatg gtcagctcca ccgaggccac actgctgacg atgatgacca tgcgacaggt
    32341 ctgcgaacgc cctgagcctt cggcaagagc tggcttataa ctttcggcca tgttcgccac
    32401 ggcaattaac gttcccgcat catcgtacag gccaagctca cgcatccaga aaccgcccac
    32461 ctccggcgga ataaccagct ctgcgataat ataattactg tttcgtttgt cctggctgat
    32521 tttgttcagc gtatgtcgcc agacttcgtg gataagcccg gtctgtccgg catccgggac
    32581 aggcaattta ccaccgccat ccccgacggc catcgtggta atgttgacct tccgccctcc
    32641 cggtgcggtt gccgctgcca gctttgctgc accggcagtg gtgataacgg ttctgaattt
    32701 tgtgctcatt attcctcact tatccggggt aaaccgtaat tacatcgccg tcgtaagcca
    32761 caccaccggc gaacaggtag ccgggaatgt cccgggtaat gttcaggcca ataagatgac
    32821 ggcttgcagg tttggcatca gcaataagcc gttccatttc ctgatacatt gcctctgtga
    32881 tgccgctttc cagtacacca atatcaagcc ggaaggtgcc gggcgggtca ctgttttccc
    32941 accactccgt cacgttgatg agatagccga gcggctccac cacacgccgg attgcgccga
    33001 tagtgccttt atgacagtgg atgaaatagg catcgcggat aacggcgcgt tttgtcgctt
    33061 ccggccactt ttcatcccac ctgtcgaccg aaaacgccca cgccagccac ggcagcagat
    33121 ttgccggaca ggtatccggg ttccacagtt cacgaatcct gaccggcgtt ttttcaattt
    33181 ccgcacaggc ttttgcggcg gcaacttcaa gcggtgatga gccggtcggc agcagtcgcg
    33241 aatcactcat ccgagcctcc ggtcacgacg cggtattcgg tacagaaaga cgcctgcgta
    33301 ctgttgagca cgatgtcggc cagcggtgca gtcagttcga cacgctgcac gccttccaca
    33361 tgcaaagcgg cataaatggc agacagacgg atgtcgcgcc ccagccggtg ctgtgccgtg
    33421 atgtacgctt ccagtttttt cacggcagca gcgcgtatgg gttcgctttc gggaccaggg
    33481 taaaggtaaa gcgtggcgtt tatctggtat tcaacgatgg cggcagactg cacggtcacg
    33541 cggtcggcca ccggcctgac gtcctcacca ttaagggcgt tacgcaccac cgccagcagg
    33601 tcttcggatg cgacaccgtt attttcacgt gacagcacag agatggtgac gcaggccgga
    33661 gacggactgg ttacagaaat atccgcgaca cgcccgtcgg cactgcgacc atgatactga
    33721 taggccccca ccgacccggc gacgcttaaa ccttcaaacg cctgctgaat acgcagacga
    33781 taatccgtgt cagattccat cactgccggt gtcggcggga gggtcgaatc atctgccggg
    33841 gtgataatca ggcgcgtggt gttgtaattg gcaccaatca catcaaggtc attaccggcg
    33901 gcacaggcca gcattaccgc ccgtgcggcc tcattcacac gctgacgcca gataagctca
    33961 cgataagcat tttcctccag cagtttgacg agaggctcgg attccagcgt cagggtacgg
    34021 gcgaccgcct cctgctggtc ttccgggtaa agggaaatca gtgtcgcctt gcgttcggca
    34081 agaatggttt caaagtccag ctcctcgacc acatccggtg cgggtagctg gttcaggtcg
    34141 ataatcggca tggtttcaac tcacagggat ggttaacgaa agtggctggc cggtgtcgtt
    34201 gtgctggccg gttaacgtga ccgtcattcg cccgtcaaag ctgcgcgccg tggtgacgga
    34261 tgacagggtg acgcgggctt cccatttcag cacggccatg taacaggcga ccttaatctg
    34321 caactcaagc gccggggtct gcggctggtc aatcattgac gccagcaacg agccgtaatc
    34381 acgacgcatc acccgtgagc cgaccggcgt gcgcaggata tcgccaatac tctggctgat
    34441 atgctcaagg tcagagacag tcaggccatc actgcgattc attccgagat aacgcgctgt
    34501 catagaggac tcccggttgt gccgccgctg tcgccggggt gtttatgggt atgcagtacc
    34561 ttcccgtttg atgagagttc accgccggtg tgttcaatgt tgccgcgcat cgtcccgccc
    34621 ttctgcactt ccagcgtgcc agtgatcagc ttgtttgtgc agaccacctc cggcgtgtcc
    34681 agagtgacgc gggttgatgc tttcaccgtg accaccggca ccgtggcagt aacagaatca
    34741 gaagccgtca cgctggccgt tttaattccg cttaccgtga gtgcactggt ttcaggttca
    34801 tactcaatca ccgccccgtc agggaaacgg atatgcaggg catccgccga cgcagacggc
    34861 gcggggttat caccggaata aatccccggc agaacgaacg ccgtgtcgag ttcaccgcct
    34921 acggccagaa tcagcacctg ttcccccacg gaaggtgccc accatgtgcg cgaacgtccg
    34981 gcgcgatggg tcagccactg aagccagtcg gtgcacatgc cgccggtctg cacacggcag
    35041 cgaccggcat taaggtcggt ttcgacgata atgccggtgc ggatcatgtt gcgcagtgcg
    35101 cgcgcgagtt cctgaatatt tgcgagagtg ttcataacgg gaaggatgcc gccgggtcat
    35161 accggcggca atgtgacgat gaggtgtcag gaatggcaca actaacggtc gaggtgagcc
    35221 aggataatct cttcaatcat ctgcacatcc tcaccggtaa agccgagcag gggacgcgcc
    35281 ggataatgaa ttttcttacc gtctttccgg gtttcttccg acagaccgaa ctgatgcaca
    35341 ctggctattt tcggtgactt cccgccgtaa aactccattg atgcctgttc agggctggcg
    35401 cggatatgca aaaaacgact ggtgataagt ttcgcaaaca tttttcgctt aacgcgaccg
    35461 gtcttttttc tggcgctctg ctgctggcgt ggtgcgtagg gtgtgccgtc cggggctttc
    35521 tgtgccatca cccgacgctg ctgactctga cgcagacgtt tcgccagttc ggcgctcagt
    35581 cgccgacgcc ctgacggtga cagcgactca ataagtccgg tcagcctgtc ttcaaaacgc
    35641 ttaaactcat tcatcccact tactcaccag ttcgccattg atataaagct ccatcgggcg
    35701 ggtgaccggc tccggcggcg gaggttccgg gatattcttc acatgcagcg cgccgtccac
    35761 ctcactgacc agcgtgcgct cggtcagcat caggctgatg ctgatatcaa agctgctgtc
    35821 attgttgatg tctgcataaa acgtgaagcc ctttttctgg ccttcgtcgg tggtcatgat
    35881 gtcgggctga ttttcccgca gccacgccag caccggcacg atgagcaggt caaaatcacc
    35941 ggtaaagtcg gtcacaatca cgttgagcgt gtaacgcttt tcgaatgaca gcgacgtcgc
    36001 cagtgtggag gcaatactcc cgttatccac gaatatccgc agcatctcgg ggcttgtttt
    36061 cagcaccgtg acggcatcag tcagcgccct gcgcaggctg tcgggtttga gcatcgtttt
    36121 cgtcctgaca gtgtttaatc atttttacct ggctggcaca gcgtgccagc gcgttctcaa
    36181 gctgccggat atcagcactt aaatcgccgt tcgtctgcgg gtcactgccc ggcatcgggc
    36241 aaaggctcac tttcgggcag gcgttgtgga caatcactgg cgtctgcgca ggctgtgcgc
    36301 tggtgcaacc ggcgcacagc atcaggcagg tcagcaccgt accagcggcg aaaatcttcg
    36361 ttttcattaa gtaacctcgt gatggttttc tcgcgctgag cttcacgctt cgcggcgttc
    36421 tccagttcct gacgcagtgc cacctgcgcc agctcgtttt tgtctgccct ggtgagggca
    36481 acatgaagct ggtttttcag catggtgatg gtcgtctgct gttcactggc gacgttgttc
    36541 gccctgtcca gcaaggcgcg caggctggca tttttgtgtt tcaccagaaa cagaccggcc
    36601 accgccagcg ataacaacac aaccagcaca atcatcagtt ttgacatggt tcccgcccct
    36661 caagacgctg acggcaggcc gtacgtatca gccggaaaaa cagcgatgcc acgagataaa
    36721 tcagtgcggt aaaaatccac ccggcagcga ccagcgagat aaacgtcgcc accatcacca
    36781 tcagagccgc tgcccgtctg cgccacggca ccggctgcaa aaacagcgac gtgacaatct
    36841 tcacggccag cgattccggc ggcagctccc gcccgtagcg ttccagcaca tactccgcgg
    36901 catacacgcc gacaccaccg gcaatcacac agataaccgt cgccagaatc gcccatgcag
    36961 cgacaaaact gacggccacg ctctgcgggt aaatcaggga cagtgccagc atcagcgcca
    37021 gcgacacgtt cagcatcagt gaaagggata atttcttcat ggtgtttact ccgtttaagc
    37081 cggtacgccg ccagcggtgc gccagacggt gaccagtttt tccagtgaat gctcacgctg
    37141 accgtaaccg gcacccggca gggacgccca gatattgcga cagcgtgaaa tggcgcgctc
    37201 aatgcgtccc gcccggatgt catccagtgc accgcgttcg cggatcaact gaatggcgag
    37261 cctgtcctgt gacaacggac tgaaatccgg cagggcaagc tgtttgcggt agtgcggcca
    37321 gaacaggtaa agctgctgat agcgaccgga ggccgtggat ttttcaccgc gacggttaaa
    37381 caccttcgcc ggtcggccat gtgcgaacgg gtggtcactg tagtcggtga agatttccgg
    37441 cttcccgtcc agtccggtga ctatcacgtc atagccccgg tttttcgtca gcggatgatt
    37501 cgccgtccct tcggacacgg ccagcatgtc gagaaaggcg gcgatattct gatgtgtgct
    37561 aattaccggc attacggttt ccccctgccc ttaaagcggc gctgaatggc aatctcaatc
    37621 acctgataac cggcgatacc cagcatggag ccgatgccgc acaccgcagg cagtgacagg
    37681 tcaggaaact gcaccagaac aacaccggca accatcgaga caaaaccacc gagcaacatg
    37741 cgcccgataa acagacgcgg agtgatgggt tcaccaccgg caagcacctt gccgacaaca
    37801 atcagcaccc caatcatgaa aagcgacagg acgctttttt cttctgctgt catgcgttac
    37861 tcccacagat tgacagtttc agccacgggc gcggtctgaa cgtcgggcag ttcgacggcg
    37921 gtgccgtgtg gcagcaccgc ccccagttca gccagtcccg gatttgcggc gagcacggcc
    37981 tcgaccacgc cctcagtgcg cccgtaatac cgaacacaaa tggcgtcgag cgtgtcgccc
    38041 tgtagcgcaa aggtcttcat cagatttgac tcacgatgca gcgcggcttg tcctggatac
    38101 gcgccaccgc ccagcgcata tcccgccaca gctcatcaat ggtgctgtca atgctgtcgg
    38161 ccttcttgtc gcccttggca ctggcatcca cgccgcggta acgctcataa agcgacgcgg
    38221 tcgccatcgc acacacagcg cgctcgtagt aaaaaacttt gatgctttca ccgtcgatgt
    38281 cgtccgccgg gacgtcagcc agacgcgaaa aaccggcagc aattttctgt tcgcggtact
    38341 cgtacagctc cgcattcgtc tccgccatgc ctgacttgat ggcctcacgc agacgggcgg
    38401 gggcgacggt ctgctcaagg cgcatacgtt cccggacgcg cttcgggtca atatcgggaa
    38461 aaaagaacgt gtttttaatc accggctcgt cgcctgccgg ttgcgggata accaccgtac
    38521 cctcaccgga cacgggagcc tcctttcgcg gaataatcag cgtcatcatg actacctctg
    38581 aaaagtcggg cggtggacgc cggtgcagtg tcaggtgatt caccgtcact gaccggcgtg
    38641 ccgccctggc gcggggcgca ttcggttgtt aactggcttt ctttttcggg cgtccacgtt
    38701 ttgccggtgt cacgctccgg gtcttacgcg gagtgcgggt ggccgctttg ggctgcggct
    38761 ccggcttcgg tttcagctcc cgctccagtc gttcaatctc ttttttgacg cctgcctgac
    38821 agtcgagctg tgtcgcacgt tgcaggtgta ccagcgcacc tgcggcatca ccagcgtcac
    38881 gcagaaacag accggtgatt ttgtgcagct ttgcgcgcac ttcatcaggc atgtcagccg
    38941 tggcggtcag ttcgagggtg tccgtcagca ggcggggatc cacagactca ccggcagcgt
    39001 gagcgcgcat ggccgcaagc gccacctcct cggtgaacat gtacggcggg gtgcggcggt
    39061 gtttacccgg catggtcaga ccgtacttca gggcataacg ggcaatctcc agcgcaccgg
    39121 caatatcgcc ggtatccaga cgccacagca tgaccgtcat cagaatgtca tcctgtgcac
    39181 ctttgccctg ctccagcacg ccgttcaccc acggcaacca gaacggcagc agttcgcgtt
    39241 ttttcgcggc cttcagctct tttgaataaa tcgctttcag tgtgcgctgg tctgcggcca
    39301 gcttgaccag catctgctca tagacagttg catgtcgcag cggggcggct tcccgctgcg
    39361 cggtcatcgc tgccgagacc cgcatcatgt ggcgctgtgc gggactcgtc atcggttacg
    39421 ctcccggctc tgcggtcgct ttagccggtg tggagaaatc accgacctta attttttcca
    39481 ccagacaacc ggcggcgtag tcctccacca cgtaatcaat gttcattgac tcgtagttct
    39541 ccacgcggtc gagtttcggg ttttccacaa tcacgcggcg atggctgtca tccatgtagt
    39601 agatggacag gttttccagc ttcgtgatga gcatcgcatc cgccgggaag tacgggacgc
    39661 gtaccgccgg caggttaccg atgcgtttct ggctgatgat gacgtcagcg gccagcatct
    39721 cgctgttatc ctgctccttg ttgacgatag ggaaatactt gtccgccagt agctgacgcc
    39781 ccacaatcac cacaaggtca gggtcttcct gataccaagg ttcaatcagg ttgttggtcg
    39841 catccatcac cagtgcatcg aggcttacat aatcaccgcc cttacccacg cggataacct
    39901 cagaggtggt gcggccttcc tcgtcagtga ccttgctcat cacgcgcgcc ggggcttcat
    39961 tcggtatttc tgcagccagc cgaccgccac atcctgcagc atcggattac tgttgcggtc
    40021 agaggtttcg gcacgcttca cgccgttaaa accggccatg atgaaatcaa gggactggcg
    40081 tttgataatg gcgttacgga tacggagctg gaaatcctga taacgcgccc acaggtcaag
    40141 cgttttgtag cggatataaa aatcgaagtt aacctggtcg cattcgtact tgtttgacgc
    40201 cagcttcgag aagtccttcg gctgacgctc ggtgccaccg gcggtgtcgg tggtgctggc
    40261 gatggagccg gtgacaccga tgccaatttt ttcccctttc atttcactga ccggcacaat
    40321 gttgatgcgg gtcagaaagt cagaggactc ctgcatggtg ttcatcaggg tctgggtgac
    40381 cgacggttca acggtgaatt ttttcgacac atcaccggcg tcgatgccgt tcagctcggc
    40441 aacacgggac aggtaagcat taaatttaaa gcgggtttcc tggcgcatag tttttcctga
    40501 aattaagggt taatcgtgaa ggttttcccg gactgactga cgccggtcag cagttcgtca
    40561 tcagggcgtc accgccacca ccggtggcct tgctgcggcg ctgctgggtc agactttcgg
    40621 tgtggtcgag actgtttttc aggcgggtga atgcctggct ggtttcatcc gccctgtcag
    40681 tcacctcctg cttaagtgca gaaaaggcgg tttccatctc agcgaggcgc tgctcagtgg
    40741 cgctcagttt ttcctgcaca tgttcagcaa cagcggtcac cgcttcatgc acgtcattca
    40801 gacgggcatc atcgctggcc tgtttgcggc caaaaatgga tttcaccttt tcggtcaggg
    40861 cggtgaacac ggtttcaggc aggtcttcaa attccagctc aacaggcgtt gccactgaaa
    40921 tcaggttttc agggcttaat ttgaagcggt tcagggggtt gtgttttgcc gtgcggcaga
    40981 attccaggta ttccgtgccg aggcttgccg ggtcatcggt gacggccaga cccaccagat
    41041 aacatttgcc ggtgttggca aagttcggct gaatttccat tgaggtgtag accttctgcg
    41101 cggccttgtt catcgcgata aggtcatcgg tcggggtgat tttcgcaaac agcgcccatt
    41161 tgcctttcag cgccgaatca tcgtcaatct tttcggcctt cagttcggcc acatcgccat
    41221 aacgcttaaa aataccgtca ggcaggatgc cgcgcagatg ttccaggtta atgcggcaac
    41281 catagacacg cgggtcaaag gtttcggcca tttcctgaat atcctgcgca ctgatgacac
    41341 gcccgtcaca ggtgtcaccc tcaacgccga tacgaaagaa ttttgagact ttttttgcca
    41401 ttgtcaggag tcctgaatag tgattagagg agtcacatgt cggcatcagt ttcccgacga
    41461 tgcgcatcct ccgccatcag tcccggatgg tttatcactg acacaacagc acattagcga
    41521 atcgcggggc gcgactcagt agccttgccg tgtattcatc acggcgaggt attcatgacc
    41581 atcaccacag acaccactct tttacacgac ccgcgtcgtc aggcggcgct gctgtactgg
    41641 caggggtttt ccgtgccgca gattgccgcc atgttgcaga tgaaacgccc gacggtgcag
    41701 agctggaaac agcgcgacgg ctgggacagt gttgccccca tcagccgtgt cgaaatgagt
    41761 ctggaagcgc ggctgaccca gctcatcatc aaaccgcaga aaaccggcgg tgacttcaag
    41821 gaaattgacc tgctcggacg ccagattgaa cgactggcac gggtaaaccg ctacagccag
    41881 accggcaacg aggcagacct taatccgaac atcgctaacc gcaacaaagg cgggcgtcgc
    41941 aaaccgaaaa agaatttttt cagtgacgag gccatcgaaa agctggagca gattttcttt
    42001 gagcagtctt tcgactatca gttgcactgg tatcgtgccg ggcttgagca ccgcatccgc
    42061 gatatcctga aatcccgcca gattggcgcg acgttttatt tttcccgcga ggcgctgctg
    42121 cgcgccctga aaaccggcca taaccagatt tttctgtcgg ccagtaaaac gcaggcgtat
    42181 gtgttccgtg aatacatcat cgcctttgct cgtctggttg acgttgacct gaccggtgac
    42241 ccgattgtcc tgggcaataa cggcgcaaaa ctgatttttc tcggcaccaa ctccaacacc
    42301 gcacagagcc ataacggcga cctgtacgtc gacgagattt tctggatccc gaattttcag
    42361 gtactgcgta aggtggcatc aggtatggcc tcacagagtc acctgcgctc gacctatttc
    42421 tccaccccgt ccacgctggc gcacgacgcc tacccgttct ggtcgggtga actgttcaac
    42481 cggggacgcg ccagcgccgc cgaacgtgtg gaaatcgacg tcagtcataa cgcccttgcc
    42541 ggtggtcttc tctgtgcgga cggccagtgg cggcagattg tcaccattga ggacgccctg
    42601 aaaggcggct gcacattgtt cgacattgag cagctcaaac gcgaaaacag cgccgacgat
    42661 tttaaaaacc tgttcatgtg tgaatttgtt gacgacaagg tatcggtgtt cccgttcgag
    42721 gagctgcaac gctgcatggt cgacacgctg gaagaatggg aagactacgc gccgtttgcc
    42781 gcaaatccgt tcggctcccg cccggtatgg attggttacg acccgtcaca ccgtggcgac
    42841 agcgccggat gcgtggtgct ggcaccgccg gtggtggccg gtggcaaatt cagaatactt
    42901 gagcgtcacc agtggaaagg catggacttt gccacccagg cggagtccat ccgcaaactc
    42961 accgaaaaat acaacgtcga atacatcggt attgatgcca ccggcctcgg tgtcggcgtg
    43021 ttccagctcg tgcgctcgtt ctatcccgcc gcgcgcgata tccgctacac gccggaaatg
    43081 aaaaccgcaa tggtgctcaa ggccaaagac gtcatccgcc gtggctgtct ggaatatgac
    43141 gtcagcgcca ccgacatcac cagctcgttc atggctatcc gcaagaccat gaccagcagc
    43201 ggacgcagtg ccacctatga ggccagccgc agcgaggaag ccagccacgc cgacctcgcc
    43261 tgggcaacca tgcacgccct gttaaatgag ccactcaccg ccggtatcag cactccgctg
    43321 acatccacca ttctggagtt ttactgatga gcaagaaaaa agggaaaaca ccgcgacctg
    43381 cggcaaaaac aatcaccgcc agcgccccga aaatggaagc attcaccttt ggtgagccgg
    43441 tgccggtact cgaccgccgt gacattctgg attacgtcga gtgcatcagt aacggcagat
    43501 ggtatgagcc gccggtcagc tttaccggtc tggcaaaaag cctgcgtgct gccgtgcatc
    43561 acagctcacc gatttacgtc aaacgtaata ttctggcttc aacgtttatc ccgcacccgt
    43621 ggctttccca gcaggatttc agccgttttg tgctggattt tctggtgttc ggtaatgcgt
    43681 ttctggaaaa gcgttacagc accaccggta aggtcatcag actggaaacc tcaccggcca
    43741 aatatacccg tcgtggcgtg gaggaggatg tttactggtg ggtgccgtcc ttcaacgagc
    43801 cgacagcctt cgcgcccggc tccgtgtttc acctgctgga gcctgatatt aatcaggagc
    43861 tgtacggcct gccggaatat ctcagcgccc ttaactctgc atggctgaat gagtcggcca
    43921 cgctgttccg ccgcaagtat tacgaaaacg gcgcacatgc cggatacatc atgtacgtca
    43981 ccgatgccgt gcaggatcgc aacgatatcg aaatgcttcg cgaaaacatg gttaagtcga
    44041 aaggccgcaa taactttaaa aatctgtttc tctatgcccc acaggggaaa gccgacggca
    44101 ttaaaattat cccgctcagt gaagtggcaa cgaaggacga tttttttaat atcaaaaaag
    44161 ccagcgccgc tgacctgctg gacgcgcacc gcatcccctt tcagttgatg gggggcaagc
    44221 cggagaacgt cgggtcgctg ggtgatattg agaaagtggc aaaggtcttt gtccgcaatg
    44281 agcttatccc gttacaggac aggatccgcg agataaacgg ctggctcggt caggaggtca
    44341 tccgctttaa aaactactca ctggacactg acaacggctg aacatcgccg cctgcgggcg
    44401 gcttttttac accccgccat cacgccctca cacgctcatc accgcacaaa aaatcccgca
    44461 gacacaccaa cgcccaacgg gcagactaag cgccgtcacg acgcgctgag acgctggaaa
    44521 ataaaatcag caccaccgcc agcgcgcagt gctttccccg cctcgcccgc ccgcttcatg
    44581 gggcggtttt aatgcagttg catgaacact ccaagagcac gccagcgcta gtgataacta
    44641 acaataaaag cgacttctga cgcatgcaaa tccatgcaca tgatggcatt tttcatgatc
    44701 tatgcggtat gctatccgct tcaattttta acacttgtta agggaactag acatggacat
    44761 tattgattta aaatctcaac tagaaaatga agataatgcc aagaaaagag cagagttgct
    44821 tgctcagcta gaggtgataa agcaaaacgc tgaagagcaa attcgtgctg agcaaaagga
    44881 tgtggatttt gagaccaaag aatttaccgt tgagttatta gttaataagt atcataacgg
    44941 attagaggat gatactaacg aactctttgt tcctgattat caaagggatt ttgtttggag
    45001 tgaaaaaaga caatcaaaat tgatagagtc tttgattttg ggttttccta taccatatat
    45061 atttatagca gatgttctct ccgaagatcc cgatttagat gggcgaattg agattgttga
    45121 tggctcacaa cgagttagga ctatacacgc ttttatccaa aatgaattag ttttacaaga
    45181 cctgaaatct ttagacgtcc ttaatggttt caaattccaa gatttaccat tatcacgtca
    45241 acgtcgtttc atgagaattc ctgtccgcgt tatagaattg agttctaaat gcaatgaaga
    45301 aacccgtaga gatctttttg agcgtattaa ctctggcagc gatatattga aagatatgga
    45361 agtccgtaaa ggatcagaac ttggttcaac aaatttatat acacaggtta ttaagccatg
    45421 ctcaacaata cctaaattta aagaattagc acctttatct cagactaaag aaaaacgaga
    45481 tgaacggtta gaatttacat taagattctt tgcctacgta gataagtaca agaactttga
    45541 tcattccgta cgagattttc taaatgacta tatgtcagaa aatcgagata tagacgaaac
    45601 taaacaaaac tcaatgaaac aagagtttgc taatgttcta tcttttgttg aaaactattt
    45661 cccagcagga ttcaagaaaa ctattactgc aaaatctaca cctcgagtta gattcgaatc
    45721 tctcgctgtt ggcgcagcac ttgcattacg tgaagtccct gaccttgttc ccaagaactt
    45781 agattggcta agctcagaag aattcaaacg attgaccact tccgacggag ctaatagtcg
    45841 cgtgaaggta attgaacgaa ttgaatatgt tcgggataaa ttattagagg attaacatgg
    45901 atcgttttcg tgaggatttt gacgaaaggt caggagagat cttggcctat ttggatcttt
    45961 tgaaatttat tgagtatgcc ggtgcagaac tgatatcagc tgatgataaa gaacataagt
    46021 tctccattac tgctcaatca cgaaaaactc taaagggagc agtatatata ctgttatata
    46081 acctcattga atcaacaatg agagaagcta tctgccttat tcatgagact atttatgata
    46141 gaaacgtaga atttgacaaa ctcaggaaaa atctcaggtc agaaatatta aaaagattaa
    46201 aaaatgaatc tgtaaatgtt gaaagtttag ttaatcacct
//
LOCUS       DAZPDU010000011        44697 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZPDU010000011 DAZPDU010000000
VERSION     DAZPDU010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44697
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.011"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..716)
                     /locus_tag="ACHURS_000558"
     CDS             complement(<1..716)
                     /locus_tag="ACHURS_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1236375.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSSA"
     gene            complement(730..1389)
                     /gene="pgmB"
                     /locus_tag="ACHURS_000559"
     CDS             complement(730..1389)
                     /gene="pgmB"
                     /locus_tag="ACHURS_000559"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFR1236376.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVNAVLPGIRSLL
                     ADLRAQQISVGLASVSLNAPTILDALELREFFTFCADASQLKNSKPDPEIFLAVCAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGITGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(1386..3653)
                     /gene="ycjT"
                     /locus_tag="ACHURS_000560"
     CDS             complement(1386..3653)
                     /gene="ycjT"
                     /locus_tag="ACHURS_000560"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFR1236377.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTAATHQEDE"
     gene            complement(3650..4705)
                     /locus_tag="ACHURS_000561"
     CDS             complement(3650..4705)
                     /locus_tag="ACHURS_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR1236378.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPLAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRKMADDNRHLRSMEAFINHVQGKPVMIADAKQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(4715..5503)
                     /locus_tag="ACHURS_000562"
     CDS             complement(4715..5503)
                     /locus_tag="ACHURS_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFR1236379.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGDKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(5521..6573)
                     /locus_tag="ACHURS_000563"
     CDS             complement(5521..6573)
                     /locus_tag="ACHURS_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFR1236380.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKVRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSAFRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(6604..7446)
                     /locus_tag="ACHURS_000564"
     CDS             complement(6604..7446)
                     /locus_tag="ACHURS_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFR1236381.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKGAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSAGNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(7433..8314)
                     /locus_tag="ACHURS_000565"
     CDS             complement(7433..8314)
                     /locus_tag="ACHURS_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFR1236382.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(8335..9645)
                     /locus_tag="ACHURS_000566"
     CDS             complement(8335..9645)
                     /locus_tag="ACHURS_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFR1236383.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTHVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKTVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDMPGSNDIMEVKDAFMNGTAPMAIYSTYIL
                     PAVIKEGDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKAEETEAAEKFVTFMEQADNI
                     ADWVMMSPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNF
                     TRMGDVTGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(9659..11190)
                     /locus_tag="ACHURS_000567"
                     /pseudo
     CDS             complement(9659..11190)
                     /locus_tag="ACHURS_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
     gene            complement(11402..11716)
                     /gene="pspE"
                     /locus_tag="ACHURS_000568"
     CDS             complement(11402..11716)
                     /gene="pspE"
                     /locus_tag="ACHURS_000568"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFR1236384.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(11791..12012)
                     /gene="pspD"
                     /locus_tag="ACHURS_000569"
     CDS             complement(11791..12012)
                     /gene="pspD"
                     /locus_tag="ACHURS_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFR1236385.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(12021..12380)
                     /gene="pspC"
                     /locus_tag="ACHURS_000570"
     CDS             complement(12021..12380)
                     /gene="pspC"
                     /locus_tag="ACHURS_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFR1236386.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(12380..12604)
                     /gene="pspB"
                     /locus_tag="ACHURS_000571"
     CDS             complement(12380..12604)
                     /gene="pspB"
                     /locus_tag="ACHURS_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFR1236387.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRDELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(12658..13326)
                     /gene="pspA"
                     /locus_tag="ACHURS_000572"
     CDS             complement(12658..13326)
                     /gene="pspA"
                     /locus_tag="ACHURS_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFR1236388.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            13493..14470
                     /gene="pspF"
                     /locus_tag="ACHURS_000573"
     CDS             13493..14470
                     /gene="pspF"
                     /locus_tag="ACHURS_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFR1236389.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNAVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQKGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(14588..15853)
                     /gene="puuE"
                     /locus_tag="ACHURS_000574"
                     /pseudo
     CDS             complement(14588..15853)
                     /gene="puuE"
                     /locus_tag="ACHURS_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
     gene            complement(15891..17171)
                     /gene="puuB"
                     /locus_tag="ACHURS_000575"
     CDS             complement(15891..17171)
                     /gene="puuB"
                     /locus_tag="ACHURS_000575"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFR1236390.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPYYPFPGGCTLRVPFTAMGAAWYSLRDRLGV"
     gene            complement(17173..18663)
                     /gene="puuC"
                     /locus_tag="ACHURS_000576"
     CDS             complement(17173..18663)
                     /gene="puuC"
                     /locus_tag="ACHURS_000576"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFR1236391.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDHAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEEAGLPDGVL
                     NVVSGYGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANI
                     VFADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGH
                     PLDTVTTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVDVDPNA
                     SLSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSV
                     FVNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(18813..19370)
                     /gene="puuR"
                     /locus_tag="ACHURS_000577"
     CDS             complement(18813..19370)
                     /gene="puuR"
                     /locus_tag="ACHURS_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFR1236392.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(19397..20161)
                     /gene="puuD"
                     /locus_tag="ACHURS_000578"
     CDS             complement(19397..20161)
                     /gene="puuD"
                     /locus_tag="ACHURS_000578"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFR1236393.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            20373..21734
                     /gene="puuA"
                     /locus_tag="ACHURS_000579"
     CDS             20373..21734
                     /gene="puuA"
                     /locus_tag="ACHURS_000579"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFR1236394.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRDAEGNLQPPC
                     APGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIPADGAVAEASPGQFEINLYH
                     TDNVLEACDDALALKRLVRLMAGKHKMHATFMAKPYEEHAGSGMHIHISMQNNRGENV
                     LSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRRFQPGMYVPTQASWGHNNRT
                     VALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILHGLDNELPLQEEVEGNGLEQ
                     EGLPFPIRQSDALGEFIENDHLRRYLGKRFCHVYHACKNDELLQFERLITETEIEWML
                     KNA"
     gene            21883..22047
                     /locus_tag="ACHURS_000580"
     CDS             21883..22047
                     /locus_tag="ACHURS_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1236395.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            22037..23422
                     /gene="puuP"
                     /locus_tag="ACHURS_000581"
     CDS             22037..23422
                     /gene="puuP"
                     /locus_tag="ACHURS_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFR1236396.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            23556..23801
                     /locus_tag="ACHURS_000582"
     CDS             23556..23801
                     /locus_tag="ACHURS_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFR1236397.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            24114..25757
                     /gene="sapA"
                     /locus_tag="ACHURS_000583"
     CDS             24114..25757
                     /gene="sapA"
                     /locus_tag="ACHURS_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFR1236398.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            25754..26719
                     /gene="sapB"
                     /locus_tag="ACHURS_000584"
     CDS             25754..26719
                     /gene="sapB"
                     /locus_tag="ACHURS_000584"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFR1236399.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            26706..27596
                     /gene="sapC"
                     /locus_tag="ACHURS_000585"
     CDS             26706..27596
                     /gene="sapC"
                     /locus_tag="ACHURS_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFR1236400.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            27596..28588
                     /gene="sapD"
                     /locus_tag="ACHURS_000586"
     CDS             27596..28588
                     /gene="sapD"
                     /locus_tag="ACHURS_000586"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFR1236401.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            28590..29396
                     /gene="sapF"
                     /locus_tag="ACHURS_000587"
     CDS             28590..29396
                     /gene="sapF"
                     /locus_tag="ACHURS_000587"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFR1236402.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            29464..29817
                     /locus_tag="ACHURS_000588"
     CDS             29464..29817
                     /locus_tag="ACHURS_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFR1236403.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     PEIILQELNRRSPSP"
     gene            30185..30973
                     /gene="fabI"
                     /locus_tag="ACHURS_000589"
     CDS             30185..30973
                     /gene="fabI"
                     /locus_tag="ACHURS_000589"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFR1236404.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            31118..32244
                     /locus_tag="ACHURS_000590"
                     /pseudo
     CDS             31118..32244
                     /locus_tag="ACHURS_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
     gene            32312..34246
                     /gene="rnb"
                     /locus_tag="ACHURS_000591"
     CDS             32312..34246
                     /gene="rnb"
                     /locus_tag="ACHURS_000591"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFR1236405.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRGIIARPVA"
     gene            complement(34324..34427)
                     /locus_tag="ACHURS_000592"
                     /pseudo
     CDS             complement(34324..34427)
                     /locus_tag="ACHURS_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            34481..36471
                     /gene="pdeR"
                     /locus_tag="ACHURS_000593"
                     /pseudo
     CDS             34481..36471
                     /gene="pdeR"
                     /locus_tag="ACHURS_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            complement(36453..36581)
                     /gene="ymiD"
                     /locus_tag="ACHURS_000594"
     CDS             complement(36453..36581)
                     /gene="ymiD"
                     /locus_tag="ACHURS_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFR1236406.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTGYSAKYIYSSTYARFR"
     gene            36618..36791
                     /gene="yciZ"
                     /locus_tag="ACHURS_000595"
     CDS             36618..36791
                     /gene="yciZ"
                     /locus_tag="ACHURS_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFR1236407.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            36881..37630
                     /locus_tag="ACHURS_000596"
     CDS             36881..37630
                     /locus_tag="ACHURS_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFR1236408.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            37899..38117
                     /gene="osmB"
                     /locus_tag="ACHURS_000597"
     CDS             37899..38117
                     /gene="osmB"
                     /locus_tag="ACHURS_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFR1236409.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(38243..38569)
                     /gene="yciH"
                     /locus_tag="ACHURS_000598"
     CDS             complement(38243..38569)
                     /gene="yciH"
                     /locus_tag="ACHURS_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFR1236410.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(38569..39306)
                     /gene="pyrF"
                     /locus_tag="ACHURS_000599"
     CDS             complement(38569..39306)
                     /gene="pyrF"
                     /locus_tag="ACHURS_000599"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFR1236411.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(39499..40668)
                     /gene="lapB"
                     /locus_tag="ACHURS_000600"
     CDS             complement(39499..40668)
                     /gene="lapB"
                     /locus_tag="ACHURS_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFR1236412.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(40675..40983)
                     /gene="lapA"
                     /locus_tag="ACHURS_000601"
     CDS             complement(40675..40983)
                     /gene="lapA"
                     /locus_tag="ACHURS_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFR1236413.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGVAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(41132..41896)
                     /gene="pgpB"
                     /locus_tag="ACHURS_000602"
     CDS             complement(41132..41896)
                     /gene="pgpB"
                     /locus_tag="ACHURS_000602"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFR1236414.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAVIIVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            42066..42656
                     /gene="ribA"
                     /locus_tag="ACHURS_000603"
     CDS             42066..42656
                     /gene="ribA"
                     /locus_tag="ACHURS_000603"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFR1236415.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLNTKAEKMGHLLNK"
     gene            complement(42720..>44697)
                     /gene="acnA"
                     /locus_tag="ACHURS_000604"
     CDS             complement(42720..>44697)
                     /gene="acnA"
                     /locus_tag="ACHURS_000604"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFR1236416.1"
                     /translation="EAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLRK
                     HGVVEKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSED
                     QVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKVF
                     AASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLLA
                     KKAVILGLKRQPWVKASLAPGSKVVSDYLAKAKLTPFLDELGFNLVGYGCTTCIGNSG
                     PLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNIN
                     LASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEINV
                     TRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGSI
                     KPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTRH
                     LPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESFE
                     RIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADGS
                     QEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
BASE COUNT        10538 a        11835 c        11284 g        11040 t
ORIGIN      
        1 gccgatgatc caataaatcc ggcaacaaac atattcgctg gttggttata aaccgttttc
       61 ggcgcaccaa cttgctgaac aatcccatcc ttcataatca caatccgcgt cgccatggtc
      121 atcgcttcgg tctgatcgtg ggtcacgtag atcatggtgg tattcagctt ctggtgcagc
      181 ttgctgatct ctgcgcgcat ttgcacgcgc agcttggcat caaggttaga gagcggttca
      241 tccattaaaa acacgcccgc ttcgcgcaca atcgcccgcc cgagcgccac acgctggcgt
      301 tgcccgccgg aaagcgcgcc cggcttgcgt ttcaggtact cacgcaggcc gagaatttgc
      361 gccgcccagt tcacccgctc atcaatcacc tctttggcga ttttttgcat cttcaggcca
      421 aacgccatgt tgtcgtaaac cgtcatatgc ggatacaacg cgtagttctg gaacaccatc
      481 gctatattgc gtgctttggc tggaacgtca ttcattcgtt tgccgtcgat caacagatcg
      541 ccgccgctga tctcctcaag cccggcaatc atgcgcaggg tggtcgactt accgcagcct
      601 gacggaccga caaacacgat gaactcttta tcggcaattt ccaggttgaa gtccttcacc
      661 acatgcacct ggttatcgta gattttttga atatgttgta acgaaagctg agccatgttg
      721 attcctttgc tatacgtttt gccagaaggc cgataaccgc gaccaggtca gggagtccgt
      781 tgaaggcaat agtaattgcg ccccggttat gcccgcgccg atccccaccg agcgcatacc
      841 gctggcgtta atggcgtcaa tgcccgcctg cgcatcttca atgccgatac atgcctgcgg
      901 cggcacgccc agcccggcac agacagcgag aaagatttcc ggatccggtt tcgagttttt
      961 aagttgggaa gcatccgcgc agaaggtgaa aaactcacgc agctccagtg catctaaaat
     1021 cgtcggcgca ttgagggaga cagaagccag ccctaccgag atctgttgtg cgcggagatc
     1081 tgccagcaaa gagcgaatgc cgggtagaac agcgttgacc gtcaactcgc gcaatgagtg
     1141 gacatagagc agatttttgc gatacgccag ttgcgccctc tcctgcgagt taaagtcgcc
     1201 ctctttgccc ccgtgttgca gaatgcgccg cagagactca tcgcggctga tcccttttag
     1261 ggattcgtta aactgcgcat caatgctgat gccaatttca gcggcaatct gctgccacgc
     1321 ctggaaatgc agatgcgcgg tatcggtgat tacaccatcc agatcgaaaa ttaccccttg
     1381 cagtttcatt catcctcctg atgtgtggca gcggtcccat tgaagggcaa aataaaatca
     1441 cccaaacaga aaacagattc ttcagccacg gatataagct gaccgttcaa acgcagtgaa
     1501 acgggggcag aagttcgaat cgcaatacgc tgcgcgtcaa gagtgacctg taattcgcag
     1561 ccctgccaga acagagggaa agacaactgt tgccactgct caggtaacgc cggattgaga
     1621 tgcaattcac cgtcacgcac gctcaccccg gcaaaaccct gaatcgcccc cagccagata
     1681 gcgccagttg cggcagcatg gataccatca tcacaactat gcggatcagc accaagatcg
     1741 atttcagtcc cctcgcgcca gaactgataa ctttgggtca gcaggccaca gcgtgcggca
     1801 acaatgccgt ggattgcttt acttaatgac gagtcgtgaa tagtgcgcgg ttcataaaat
     1861 tgcagattgg caagacacga tgccgctgag aactgctccg gcagcatgta attgagcatc
     1921 accacatcag cttgtttgag gatttgcatc tcgttcactt ctgcgcgtga ataatccagc
     1981 agaatggttt gcttccccgc cgccgctttg tatttcgcaa gattaatcgc cggcttagcc
     2041 ataaacgaat catcctgcgg caaaacgccg tcgggctgaa tttctggcag ccgcagttct
     2101 ttaaggaaca tttcggcacg atgaataaac gcgtcgtcgc tacagccaaa ctggcgggca
     2161 atactcagcg cctgctggac gttgtaatac gccatatagc tggtgaaggc gttattattg
     2221 acatgttcgg tatattcgtc aggcccaata acatcatgaa tttccagacg gtcgttaacc
     2281 ctcaccgcgc ggctaatcca gaactttgca gtttccagaa gtagcgccat gccttcatgc
     2341 gcaatgaaac tttcatcccc cgtggtctgc cagtattgaa taaccgccca ggcgatatcg
     2401 gccaccagat gatgttccgc ctgcgccgag gccacttttt gccgcagccc ggtgcgaatg
     2461 ttaatggcgg caaattccgg cgtctcttct tcgccgctgc gcgcgctttc ccacggaaat
     2521 agcgcgccct gccagccgtt gcgtcgcgct ttctcctgcg cgcctggcaa gttgtgccag
     2581 cgataacgca gtaaacttcg ggcaaccgtc ggatcgctaa acagatgaaa cggtaacaaa
     2641 aatacttctg tatcccagaa aacgtggcct ttgtagcctt cgccggttaa gccttttgcc
     2701 gcaatgctgc tgcgctcgtc gtgggcaggc gtcatgatgc gcagatgata aagcgcataa
     2761 tctaacgctt gctgatcgtg cgcttcgccg ccatttaccg tgatacgacg tttctgccac
     2821 cattgacgcc agttttctgt tgatactgca agaagttggt cgtaactctg ctgcgcgcac
     2881 atttcaagct ggcgaagcga cgcgctgccc cactcgtcta aaacggcttg cctgtcatcc
     2941 cgccagtcga tccacaccag tttttgcaac gtcactgtct cgcctgcatg aagctgcgca
     3001 ctggtatgtt gcagtaaacg gcgctctttg gcggtatagc attgctgcac atcaccgctc
     3061 accttacagc aacagctgat ggccacatca ctgcgtccat cctgggtggt gtagatcccc
     3121 tgcatcaaat gctgaccaaa cacccgcacc tgggtttcgt cgagatgctg gcgaccgtgg
     3181 ttggtctgcg tggcgtcaat gcctgttgaa atcagcactg acgcgtcggc gtccagtggc
     3241 gtaatagtga tttccagcac aatgagcggc agttggtctg ccgaaacaaa gcgacggctg
     3301 gcgatggtgt aacctgtgcc gttgctggta cgccagacaa cgctacggcg taattctcca
     3361 ctggcaaagt caagttcacg ctgccaggct tcgcgggata acgagaaaac ctcaccattt
     3421 atggcaatct ccatccccac gacatcaggc aggttcacca gttcgttgat ttcacctttt
     3481 cccgcccgat gatacagccc cgccagatac atccctcgcg tctggcgcgt gtaatcttct
     3541 tcatggctgg cgcgaagccc aagatagccg ttcccctgcg ccatcagcga tgcatacttg
     3601 ttcagggtat gctggctgaa atggggttct gataacgtta ctggcctggt cataattcca
     3661 cacgcgtccc tgtttctgcg gattgataca acgccgccac cagttgctgg atgatgtacc
     3721 cctgcttggc gtcggctatc atcacgggct tgccctgtac gtgattgata aaggcttcca
     3781 tgctgcgcaa atggcggttg tcgtctgcca ttttccgttg catcagcgtc attaattcac
     3841 cgttgttatc ggtgtagata tgtgctggaa acagcgtcgc accagcttta tcaccacaaa
     3901 agctgacgtt cataatcgac tgttcgcgga tgttgagtgc aaatgacgtt tccagccaca
     3961 gaatgccgcc gttatgaaat tcaatggtgc caaacagcga atcttcgacg ctgtaagttg
     4021 ccggatccca ctcaccaaat tgaccacagc tcttttgcgt gccgatcttt tgaaagctat
     4081 gcgcattcac gcttttcacc gccggaaagc ccagcacata catcgcagca tccagcatat
     4141 gaatgccgat gtcgatcagc gggccaccac cctgcagttc tttattggta aagacacccc
     4201 agccgggaac gccgcagcga cgcagggcgc gggcggtggt aacgtaaatc tctcccaaaa
     4261 cgccgttggt cacctgttca cgcagctgtt gcgtatcgag cgcaaaacga tggtgaaagt
     4321 cgtaggccag caccttgccc agtttgcgcg cggtatcgca catttcccgc gcctgttctg
     4381 gcgtcatggc gagcggtttt tcgcacatca catggcagcc cgcttccagt gccatcaggg
     4441 tatgttcgta atgaaaacgg ttaggtgagc agacgctaac cacatcaggt ttcaccgcca
     4501 gcagcatggc ctgcggatcg tcccacacgg atgcattccc gtatttttct gccagcgcct
     4561 gcgcctggga aaggcggctg tcacagacag ccaccagttc cagatcgttg cgggtgcagt
     4621 agtacgaagc atgaacctta tccgccacct ggcctgcgcc tattatcgcg acccgcagcg
     4681 gagagcttgt cattgcactt ttcacaaaga cctcttagca ggtacgcaac caggccaacg
     4741 aatcacggta cgcctgggca ggatcttccg cccggatacg cccttcgtac actacatagc
     4801 cctgatagtt atccgcgcgc agctgttcaa acagcgcgtg gaaatccagg gtgccgctgc
     4861 ccggctggta gcgatggtta tccgcaatat gcacatgacc gagcaggtcg cggttgtcat
     4921 gcagcgcctg cgccaggtta tcttcttcga tattcatgtg atagaaatcg ccgataatct
     4981 gtacatgttt aagatcgttt tcgacgatgt aacggcgggc atcggcgagg gtgttgatca
     5041 tatgatcctg atagcggttt aacggttcga gatacaccac tgttccggta cgcgcggcga
     5101 cctgttccag tacgcgcagg gaatcactca ccattttgcg gtcgccgtcc aggctacgcg
     5161 gcgaggtcat cggcggtaag cggaaggtaa acatgcccca cgctgccgga acgacgatac
     5221 ctttatcccc cacttctgcc agcgcttcga gaatgcgttc gatctgcttt aagccattaa
     5281 gacgacgctc ttcgataaag tcgccaatcc aaccgtcata gccaccgcag gcggtggtca
     5341 ccggtaaacc ggtttctttg attgctgctt tgacttcttc gagattgttg accagcagtt
     5401 tgccgtcaat ctcaaaacca tcgaagccca tctctttgat ataacgaaat ttctccagaa
     5461 tgttttccgg aaagaatgcc tgattctgtg tgcctatttt cattttttca ttcctttgaa
     5521 ttaaaacgta acgcccattt tgatgctctg ttccggatgc tgatcgacat actgcatgta
     5581 gctttccggg ctattggcaa aggtcactac cgggtcgatt aaatcttcgc aattgagata
     5641 accgttcatc agcagttccc agcaggtttc ttcaatacgc ttacggctcc agcgcggata
     5701 atccgggttc ggttcgctgc acgcgcggga gaaaacaatt ttggcgttat tgaaatgcgc
     5761 ttcgcgtccg aggttaaaac cttcagcaaa tggtttggca aacgcaacat aggagatggt
     5821 gccaccgtag gccagaccgc ggaacgccga ttgcagcgcg tcggcatagc cgctggtttc
     5881 gataatcaca tcggcaccct gcttgccggt cagcgttttg atctctttgc ctacgtcagt
     5941 accaatgggg ttaaggcaga aatccgcgcc gtggcgacgg gcaatatcac agcgatgagc
     6001 gataggatcg acgccaatca cgaccgacgc gccagcacgt ttagccagtt gaatggcaat
     6061 ttgaccaatc gcgccaagcc ctaccaccac aacaaaatca cccacgcgca cgttggcatc
     6121 gcgcacgccg ctcatggcaa actgcgccgg gtcgtaacaa acggcgtttt tccacgagct
     6181 gccttccggc attttgcgca gcttgtagtt attcaccgcg ttaatgatga ccgtctcgga
     6241 gagcgggccg tagccgcata cgctgtcgcc caccgcgtag tcggtaacgt cgctgccgca
     6301 ctcgataatg tcgccaacca ccatgtttcc aagctggaat ttgccaaact caatgccgcg
     6361 cggcgcatct gccggacgcg gagtgaacat ctgccattcg ccgttaaagt cttcatcgat
     6421 aaacgggctg gcggcgcgga agtcgaccac ttccgttccg tgtttcggtg cgccgaaacg
     6481 cacgcggacc ttcacttcat tagctaaaat cgcccgatct tcatactcaa ccagcgcagc
     6541 aacacgcggt gctgtggcta ctaacttttt catttaaaac tccttgtaaa gaaactggcc
     6601 gctttagccc ttcacgccac cggcggtcaa accactctta ataaaacgtt ccgaaagcgc
     6661 atacataatc accaccggca atgcggtcac cagtgaggcc gccatcatcc gtccccagat
     6721 gtaatctggc gtactgaaca gcgcgttcag gcccaccggt aaggtgaaat tgccggcgct
     6781 ggagaggaaa atcgacgcaa acagatagtc gttccacgcc accataaagc agtagacgaa
     6841 cacggatatc aacccggaca tcgccaacgg cacggtgatg cggaagataa tttgcagacg
     6901 gttgaggcca tccatcatcg ccgcttcttc gatctcatcg gggatggtgt cgaagtagct
     6961 tttcagcata aacacggcgg tgggcagagt ttgcgtcacc atggtgatga tcagcgccat
     7021 ctcagtgtcg taaatgccca gcgcggtgat gattttgaac agcggcacca ccagcaaaat
     7081 gccagagaac atatacaccg tgtaaaagct ggcgttgatg gtcatccgac ctttaaagcg
     7141 caggcgggaa agcgcgtaag ccccaagaat gccgaggaat accgccacca cggatgaaac
     7201 cacagacacc accagactgt tacggaagta gtcgacaaac gggaaaatca tcgggttaaa
     7261 aatgtcgacg taatgctcca gcgtccactg ctgcggcagc agcgtaggat gcagtgagat
     7321 cgcctctttc gcgcccttga acgaggtcat cagcatcaca aaaaatggaa acagcgtgat
     7381 gatgagaaac agcgccagcc cgcagtaaaa accgatgcga ctgagtgtgc gtttatttgt
     7441 tgccattgag gttcacccgt ttcctggtca gcagaatgac agcgaaaata atgatgaaga
     7501 gcaccaccga aatcgccgcc gctttcccta agtcattaaa ggcaaaggcg gttttgtaga
     7561 gatagacgcc gagaatatcg accttggtgg tcagcagata aacatcagca aacatgtaga
     7621 acatccagat agtgcgcagg gtcaccaccg tcgccaggac cggcataatt gcgggcagcg
     7681 tgacgatgcg aaaccgctgc caggcattag cgccatccat ctctgccgct tcgtacagcg
     7741 atttgtcgat ggtctgcaaa atcgccagaa acgagataaa ggcatacggg aagtagcgcc
     7801 agatggcgaa cagcaccacc agcacaaagc tactgcccgg attatcgaac cacagcggtg
     7861 cctgctcata gagatgcaga agatcgacgc cgaggtagtt cacaatgccg tagccgttgt
     7921 tgaacatgta tttccaggca aacaccagcg aaatggacgg cgttacgtag gagaggatca
     7981 ccagcgaacg cgcggttttg cgcaggcgga actcgcggtt gaaaaacatc gccactgcca
     8041 gcccgagcac ggtgctgccc gccaccacca gcgcggtata ccagaccgtc atccacagcg
     8101 agtgccagaa gccgggatcg gagaggatac gcacatagtt actcacccca acaaaggttg
     8161 actcgatgtt gggattgagc ggcagacgta aaaaactgat ttcgatattc gacaccattg
     8221 gccaggccac cagaccaccc agcagtaata agctgggcgc gagaagcagc agcgcaaagg
     8281 gcatatcgga acgacctgaa aaaagcctgt tcattgcgga ttcctgtcct gggtttagtg
     8341 ctgttcgatc agttcatcca gctttttctg gctcgcttgc agcgtgccag ggagatcggc
     8401 tttacccacg gtgacgttat gcaccatgga gctcaccacg ccagaacccg tcacatcacc
     8461 catgcgggta aagtttttat cccctactgc gccaaagacc tgaatatttg gcagttcacc
     8521 gattagctga ttcggtagtt cacccagcgc cttaataacg tcgttgtctt tccaggtggc
     8581 ggtagtgaca accgctttat tcaccggcag cgcagcacct ggcgacatca tcacccagtc
     8641 ggcaatgttg tctgcctgct ccataaaggt gacaaatttt tctgctgctt ccgtctcttc
     8701 ggccttttgc ccggcggtaa tggtcagcga ggtcaacatg ccgtagaccg cagagttttt
     8761 ctcggttggc accacgaaac cgacgttttt cgggtcgcct tctttaatta cagccggaag
     8821 gatataggtg gagtaaatcg ccatcggcgc ggtgccgttc ataaaggcgt ctttcacttc
     8881 catgatgtcg ttagaacccg gcatgtcgtt agaacccggc atagtgttgg cagcaaggtc
     8941 gcggtaatag gtcagtgcct gcatcatctc tggtgtatca agggtgattt tgccttcggc
     9001 gttaaagacg ttagcctggt tggataacgc aaactgggag aaggattgtt ccgtcaacac
     9061 gctttctgct gtaggcagcg caatgccgta ttttttattc gccgggtcat tcagtttctg
     9121 tgcaacgtcc agtagctgtt gccagttttt cggctcctcc agccccgctt ttgccagcac
     9181 atctttgcga taccagatgc cgccaatcca ggcgctgacg ggaacaccgg tccaggcact
     9241 cccatcttcg gtgcgcacaa tacgcagtac gccatcgtaa aacgcgcctt caccaacgtt
     9301 gctgatgacc gtggcaaccg tcttgcgatc gataagctgc tctttatcca taactttggc
     9361 gtagtcatgg ctggtttcga tcacttccgg cagcgagccg ctacgtgaaa gggtgatgac
     9421 tttggtgtta taagcgtcct cttccacggg cacttgttta acggtgatac ctgggttttc
     9481 tttttcaaaa cgggcaatca atttgctgat aactgcctga cgctcctgct ccaccgaaga
     9541 atgcataaat tcgatggata catgggtttt attttcttct ttacagcctg aaatcagggc
     9601 gcaggaaacc agtgctgata acagcacgat ttttgattta atcatgagat gttccttttt
     9661 atttaatcca cataacctgc caggggcgca gcgttatttc attacccaat agatcaactt
     9721 cactaatcag atcccgacca tgcagattag taatatttac atgctgaata tttttactga
     9781 cattaaacaa tccagtcaga caattaccat cagcgttact tctttgaata cgcattacgg
     9841 atgaattaat cgtatcgatg gtaaaattat tatccggatg aaactcgtta tggctgcgac
     9901 gaagtgtaat taaacgcgac aactcatgat ataccgcatg ccttaatgta gcttcatcgt
     9961 tcagttctcg ggttatctct ttactgtgat attttttacg gttaatcgca cggttatatc
    10021 cgagtttttc gacacctgcg taatcattac gcgagccaag aatactttga atatatatcg
    10081 ctggcacacc ggggaaactt aacaaaatcg catgggcgag aataaacctg gcgcaacgtt
    10141 cttcatcgct actctcacgg cggcttaacg catccatatg tgagaggcga ggaagttaaa
    10201 ccaggtggtg ttgctggaag gtagtgtcag gttttgcgcc cacgcacaaa gcgcctcaac
    10261 gttctgtttt tgcaccgcat gcagcaccag tggcggcagc gagaactggt acaccatatg
    10321 tgcttcgtca tcgcctgcgc caaagtaagc aatgttgtct ttatgcggaa cattggtctc
    10381 ggtaatgatc actgtacctg gcgcaacgtt atcaataatc gaccgtaaca gtttgataat
    10441 cagatgtgtt ttttccagat ggatgcagct tgttcccggc tctttccaca taaagccaac
    10501 ggcatccaag cggacatact cagcaccttt cgcaaggtaa cagagtaaaa catccaccat
    10561 cgccagcaac acttcagggc tacggtaatt caggtcaatt tgatcgtcac taaaggtggt
    10621 ccataaatgg cgcgttgaat gatcgcgcat ctggaatggc gttaataacg gtaacgcacg
    10681 cgggcgagtg acggcgctga gatcggtttg cggatcaacc gcaataaaaa aatcttcaaa
    10741 acctggctgc tgttgtaaat agtttttaaa ccattcactt tttgccgaca tatggttgca
    10801 gacaaaatca aacattaaat gactgcattc accgagttgc tgaatatcct gccactcccc
    10861 cgcttcactg gcgacctgat gataatcaat taccgaaaac ccatcatcag atgaacatgg
    10921 ataaaaaggt aataaatgaa catgtgaaaa aatgctttgc agccattgat ggtaaaactg
    10981 attaaatgtg ggtaatggtt ttaaatcatt gctgtgaaat tgatcggcat aggtaattaa
    11041 cacgacatca ctttcatccc agtgtttttt acgtcgctgt gtaattaatc gtttcgcact
    11101 ctcaagacgc gttacaagtt tctgtaaatg agttgcggta aatgttccac cgtagatttc
    11161 gtccaggtaa tccgtaattt tctgtttcat gttgcctttt tgtggtagcg gtcccatgaa
    11221 acgagagact actccgcaga aaataaactg tcaattgagc acaagggttt tagtcacgca
    11281 ggaaatcatt ttttaccagg gaaaaagcgt atttgtgagc tgacgcaaat aatctatggg
    11341 gatgtgaata aggtgtgaca gaaaaaaaac ggcgcataag cgccgctcat ggtgaattct
    11401 tttaaccttt gaccttcggc attgcgatgt ctttcaggcc accggcgttc tcaacgtggg
    11461 tatatcccat ctcgctaagg atctcttttg cttgccctga ctggcgtccg gcattgcaat
    11521 acactttcac ggtgtcgttt ttatccggaa cggcggtggc aatgcgctct ttcacttcct
    11581 tcaggggaat attgatggcc ccctgaacgt gctcttgctg atactgctct ggaacacgaa
    11641 catcgatcca gtgttcagcg gcgaaaacgg gcagtgaaaa caccagtgcc agagcaagta
    11701 agcctttttt aaacatgtaa gcgtccttct atggatgaaa attgccatcc attatacgcc
    11761 tgagatttaa caatttttgc gaaactcccc tcacctttta taacgctgtg ccagtttatt
    11821 agcagcccga cttaacagcg gttccagcgc cacagccagc aacattttca gcggtcggcg
    11881 agcaactgat tttatcgccc agcccgctac acccgccggg ccatagcgca gtgcggtaag
    11941 aagtaccagc ttgcctgcta atttgaaacc aggctttacc ttttgcccgg cctgttgcca
    12001 gcgagtattc ataactttcc tcacagttga cggaaacggc tacgtaacgt gaaagtatcg
    12061 gaagtgacat aacgttccat ctcgcgtaaa cgcgtttcac ttgccgccag ttcacggtcg
    12121 acttcatcca gcaattcgct gctggaaggt agctgctcac caaaggccat gttgtccggc
    12181 attggatcaa gcgcaaatga caaaatgatg taagcaacca gggtaaacag cgccagaccg
    12241 aagaaaatcg acagcaccac caggatacgc accagtttta ccggtacatc aaaatagttg
    12301 gcaatcccgg cgcagacgcc gcggaccatg ccctgctgtg gaatacgcca taatttttta
    12361 ttgagattaa tgcccgccat tagcgatccc tccagttcgg atgttctgca tcaagaatag
    12421 attccagcgc ctgaatacgt tcgcgcatcc gttttgcttc atcagccagt tgcgctaatc
    12481 gctgctgctc actttgcgac aattcatcgc gaccagaacg attgctgtaa tgcagccata
    12541 accagatcgg taaaacaaac agcacaaaaa tggttaacgg aatagccaga aatagcgcgc
    12601 tcataagtac tccttagacg atgagcggcg gcgcgttcgg acgccgcctg gatgttatta
    12661 ttgattgtct tgcttcattt tggcttttaa ttgtgccagt tgttcgctga ttgcatcatc
    12721 ggctttcagt tcggcaaact gatcgtccag cgatttttgt ttaccgaagc tgtggctttc
    12781 tgcttccgct tccatctggt caattcgacg ttcgaaagat tcgaaacgag ccattgcttc
    12841 atccagtttg ccactgtcca gctgacgacg cacatcgcgc gacgagtttg ccgcctgatg
    12901 acgtaacatc aatgcctgct ggcgagcgcg tgtttcgctc aatttgtttt ccagctcgcc
    12961 aatctctttc ttcatgcgtg ccagcgtatc gtccaccagc gtcacttcat gttccaggga
    13021 cttaatcaga tcggtcagtt tctgtttttc aattaacgct gcacgtgcca gatcctctct
    13081 ctctttcagc agcgccagtt cggctttttc ctgccattca acttcacgcg ccgacgcttg
    13141 ttcaatacgg cgagtcagct gtttcttttc tgccaacgca cgcgccgaag tagaacgtac
    13201 ttcaaccagt gtatcttcca tctcctggat catcagacgc accagtttct gtggatcttc
    13261 cgctttctct aacagagcgt tgatgttggc attcacgatg tcggcaaagc gagaaaaaat
    13321 acccataatg ttgtcctctt gatttctgcg ttcaggattg tcctgctgaa ctgttaatac
    13381 aatttgcgtg ccaatttttt atctttttga tttataaaga tctgattgaa gaatcaacag
    13441 caacatgcca ggatgaatta gcgaattaca ctaacaagtg gcgaatttca tcatggccga
    13501 atacaaagat aatttacttg gtgaggcgaa cagctttctc gaagtgctgg aacaggtttc
    13561 gcatctcgca ccgctggaca aaccggtgct catcatcggc gaacgcggca ccggtaaaga
    13621 gctgattgcc agccgcctgc attatctctc ctcccgttgg caagggccgt ttatttccct
    13681 taactgcgcg gcgttaaatg aaaatctgct ggattccgaa ctgtttggtc acgaagcggg
    13741 ggcgtttacc ggtgcgcaaa aacgtcatcc agggagattt gaacgtgccg acggcggtac
    13801 gctatttctt gatgaactcg ctacggcacc gatgatggtg caggagaaat tattgcgcgt
    13861 gattgagtac ggtgaactgg agcgcgttgg cggcagccag ccattgcagg tgaatgtgcg
    13921 gttggtatgc gcgacgaatg ccgatctccc ggcgatggtc aatgaaggca cttttcgcgc
    13981 tgacctgctc gaccgactgg cttttgatgt tgtacaactg ccaccactgc gcgagcgcga
    14041 aagcgacata atgttgatgg cagaacactt tgccatccag atgtgtcggg aaatcaagct
    14101 gcctctgttc ccggggttta cggagcgcgc cagagaaaca ttgctgaatt atcgttggcc
    14161 gggaaatatt cgtgaattga aaaacgcggt ggaacgttca gtgtatcgcc acggcaccag
    14221 cgattatccg cttgatgaca tcattattga tccctttaaa cggcgtccgc ctgaagacgc
    14281 tatcgccgtt tcagaaacca cctcgcttcc gacgctgccg ctggatttac gtgagtttca
    14341 aatgcagcag gaaaaagagt tgctgcaact cagtttgcaa aaggggaaat ataaccagaa
    14401 gcgcgcagct gaattgctgg ggttaaccta ccatcagttc cgcgcgttgt tgaaaaaaca
    14461 ccagatttag cgaagttggg caatgcgatt cgccggatgc ggcgtgaacg ctttatccgg
    14521 catacataat caacgtcgtt tgcaggcctg ataagcgcag cgcatcagac attattgcgt
    14581 tgggctatta atcgctcagc gcatcctgca aaattttcat tgccgcatcg aattgcgcat
    14641 ccgggatggt cagcggatac aggaagcgaa tcacgttgcc gtatgcgcca caggtcagca
    14701 ggagcagccc ctgcgccagc gcgcgttgct ggattttctg tgcaatcgcc gctgacggct
    14761 cgcccgtttg cggatcgtta aactctgccg caatcatcga ccccaggccg cgtaccgcag
    14821 caatggccgg aacgctttct ttggcatcaa tcaacgtgtt tttgagacgc tggcccagtt
    14881 gattcgcgcg ttcgcagagt gattctttgt tgataatatt gagcaccgcg tgcgcggcag
    14941 ccaccgccag cgggttcccg gcgtaggtgc cacccaaccc gcccggcgcg ggcgcgtcca
    15001 taatattcgc gttaccgacc acgcccgaaa gcggcatccc gcccgcgagg cttttcgcca
    15061 tcgtcattaa gtccggctta tcggcgtaat gatccatggc aaacagttta ccggtacgcg
    15121 caaagccgct ttgcacttca tcggcaatca tcacaatacc gtgctcgtcg cacaggcggc
    15181 gaatggcggc aaccagctct tttggcgcaa cgttgaaacc gccctcgccc tgcaccggtt
    15241 cgaaaataat ggccgcgacc tgcttcgctt cgatgtctga tttaaacaag cgttcgatgg
    15301 cgtcgaggga gtcctatgtt gaaatgccgt gtaaatctga cggataaggt acgtgataca
    15361 ccgaaccagg gaacgggccg aagccgattt tgtacggcgc aacttttccg gtcagcgcca
    15421 tggtcatata tgtacgaccg tggaagccac cgctaaacgc aatcacgcca gggcgtccgg
    15481 tatgggcgcg agcaattttc accgcgtttt ccaccgcttc cgcaccggta gtgaagaacg
    15541 cggttttggc ctgcccgctc accggggcaa gggcgttgat tttctccgcc agggtgacgt
    15601 agctttcata cggcacaatc tgatacgcgg tgtgggtaaa ctgttgcagt tgctgctcca
    15661 ccgccgcgac cagatcaggg tggcgatgtc cggtattcag caccgcaatg cctgcggcga
    15721 aatcgatgta ctcgttgccc tcaacatcct tcagagtagc gttttcagcc gactgggcga
    15781 agaagttaca catcacgcca accccgcgtg gggtagcaga aagacgacgc tgatggaatt
    15841 cattgttgct catgtttcac accttttgac tcttcactgt taatcggaaa ttaaacgccc
    15901 agacgatcgc gcaggctgta ccaggctgcg cccatcgcgg taaacggtac tcgcagcgtg
    15961 cacccgccgg ggaacgggta atacggcaga ttggcgaagg catcgaaacg ttcggcgtcg
    16021 ccgcgcagca gttcggcaat caaacgtccg gcgagatgag tacaggtgac gccgtggccg
    16081 ctgtaaccct gcatgtaata gatgttggta tcaaggcgac caaactgcgg catgcgcgac
    16141 agggtcagca ggaagttgcc cgtccagcgg tagtcgattt tgacgccctt cagttgcggg
    16201 aaggttttca gcagtttcgg cacaacaaga cgctcaacgt catccgggtc gcgcgcgccg
    16261 tagaccacgc cgccgccgta cagcaaacgg ttgtcggcgg taagacggta gtaatcaagc
    16321 agataattac agtcttccac acagtagttt ttcgggatca gcgaacgggc taaatcttcc
    16381 gacagccgtt cagtggtgat cacctgcgtg ccgcacggca tgctgcgttt cgccagttcc
    16441 ggctctactt tatcgcccag atacgcattc ccggcgacaa tcacatactt cgccgtcacc
    16501 tgacctttgg cagtgcgcac caccgctggc gtggtgtgct ggatctgcgt cacggcagaa
    16561 agctcataca cgcgcccgcc gttcaggcgg atggcatcgg cttcaccaat ggcgaggttt
    16621 agcgggtgaa tatgcccacc gctgtgatcc aacagtgcgc cagtgtagcg atcgctggcg
    16681 acctcgcggc gaatggcgtt agcgtccagc agctccagct gtttattgcc gtagcgttcc
    16741 cagttctctt tctgctcttc gagtgtggcg agctgtttat cattcatcgc cacaaacagg
    16801 ccgccggggc ggtagtcgca gtcaatctga tagcgtttga tacgttcgcg gatgatctcg
    16861 ccaccctcga acatcatgct gccgagcata cgggcggtgt ccatgccgta gcttttttcg
    16921 atcacgtcga tgtcgcggct gtaggagttc acaagctgcc cgccattgcg cccgcttgcg
    16981 ccaaagccga tgcgtgaggc ttcgagaacc actacgtcaa agcccgcttc cgccagatgc
    17041 agcgcggagg agagcccggt atagccgccg ccaaccacgc aaacgtcgca ggtgattgac
    17101 tcattcagtg tgtcgaatgg tgcatattta ttcgcactgg cggcgtagta actgctggta
    17161 tgttcggtca tttcaggcct ccaggcttat ccagatggtt ttcagttcag tgaatttttc
    17221 aagggcatgc agggatttgt cgcgaccgtt gccgctctgc ttatagccgc caaacggcac
    17281 ggtcatatcg ccgtcgttgt agttattgac gaagacggaa ccggctttca ggcggcggct
    17341 catacggtgc gcgcgggaga ggtcgcgcgt ccataccgcc gcgccaaggc cgtactggct
    17401 gtcgttggca agctgtagcg cctggtcttc tgatgtgaaa cgcgtgacca ccagcaccgg
    17461 accgaaaatc tcttcgcggc ttaaggacgc attcgggtca acatccacaa agatggtcgg
    17521 gccgatggcg gcagccagct cagcgttacg accatccagc agcagttgcc ctttgctttc
    17581 gccttcacga ataaagctat ggaccgagtc ggcgtgggcg caatcgatta aggtgcccat
    17641 ggtggttacg gtatcaagtg gatggcccgg ctgccagttt tgcgcctgct gttttaacag
    17701 ggctaagaat tcatcggcga tgctctcttc cagtaacaaa cgcgttccgg cgatgcacac
    17761 ctggccctgg ttgtagaaga tgcctgcggc ggtggcgctt gccgcttttt gcagatccgg
    17821 acaatcggcg aaaacgatgt tggcgctttt gccgcccgct tccagccaga cgcgtttcat
    17881 gttgctatca cccgcgtctt tcagcagttg tttacccgta cgcgtggagc cggtaaaggc
    17941 gatggcatcg atatcgttat gacgcgaaag cgcctgcccg gcttcatgac cataaccgct
    18001 cactacgttc agcacgccat ccggcagccc cgcttcttct tttgccagcc cggcgagacg
    18061 aatcgcactg agtggtgatt tttcagacgg ttttagaatc acgctgtttc ccgccgccag
    18121 cgccgggccg agtttccagc aagtcagcag cagcgggaag ttccacggca cgatggcggc
    18181 aatcacgccg accggttcac gcacgatcat cgccagctca tggctactgg tggtcgccac
    18241 ttcgccatac actttgtcga tcgcttcggc gtaccagcga atggcgcgcg ccgcgccggg
    18301 aatatcatca cgcagactgt gacgaatcgg tttgccggtg tcgagagttt ccagcagtgc
    18361 cagctcttcg gcgtgggctt ccattaaatc ggcgagttta ttcagtaccg ctttacgttt
    18421 agccggagaa gagagtgacc agtcgccgcg ttcaaatacg ccgcgtgctg cgctcaccgc
    18481 atggtcgata tcgacgctct tgccgcgggc aattttcgcc agcggtgcct gggtgaccgg
    18541 atcaacggtt tcaaaggttt cattttccgc cgcagcagta tattcaccgt taataaataa
    18601 gcggttttca atggcgagac ttaacgcttt atcctgccag taagccagat gatgaaaatt
    18661 cattatgact cctgtttcac gtctatcaga tatatgcagt ttgtgaacgt cagggcgcag
    18721 agatccccgg gccgcatgcc cgtttatttc tggcgacatt ctttatggat tgccgggcgc
    18781 attgcgctta cccggcttac agaacaaaaa gattaaaacg tggtgggcgt atgggcgctg
    18841 ataattcggc aaatacctgc cgacgtatta ctgaaactgt gcgggatgcc ggtattaatg
    18901 gcataacttt gccccgcgac gaggtggtaa tcctgaccat taatcgtcag aacaatttca
    18961 ccttccagta ctgtgcctat ttcctcaccc tgatgcttaa ttctttcccc agtggttgtg
    19021 cccggctggt acgtttcaaa gatcatcgcc agcgtgcgat tcgggttacc gttatgaacc
    19081 agcttcattg acacaccctg actgcccatc tcaattaagt cgtcctgatt aatgacgacc
    19141 tgcggctcat caggtttttc cggctcggaa aagaattccg agagtgacag accatacacc
    19201 ttcagcagct tttgcagcgt actgatggca gggctgactt tatcttgttc tatcgtactg
    19261 atagcactgt gagtcagccc ggagagttcg gcggcacgac gttgtgaaag cccctgctgc
    19321 tggcggattt ccgacaagcg ttttcctggc gccagtccct catcactcat atttgcattt
    19381 ccttaaactg tagtggtcag agtcgctgtt tttcagcgat atggtgctga caagcggtga
    19441 taaagccctc gaacaatata cgcgaaagcg cgtactcgct actgttccat tccgggtgcc
    19501 actgtacgcc cagcgcaaaa ggatgattga tgacgctaac cgcctcaact aagccatccg
    19561 gcgagcgagc ttcaacacgc aaccgtgggc taacgacctt cgccccttgt ccatgtagag
    19621 agtttaccca aaagttgcta cattcaggta acaacgcaga cagtaatccc ccctcttcaa
    19681 cctgaacttc atgcgacggt gcatattgct gttccaccgg cagttcggga tcttcccgat
    19741 gttccagcaa ttcaggctgt tcgcacagct tgcgatgcaa cgaaccaccg gttgccacca
    19801 ccagttcttg taaaccccgg cagatggcga aaatggggat gcgcctttcg agtgcggcat
    19861 taattatcgc catgctcaga agatcacgcc cgggatcggc gtcaggctca tcgccgtttt
    19921 caccatatag gtgcggctgc acattgctgg gactaccagg aagataaatg ccatcgagtt
    19981 tcggcaaaag ttgttcaagt aacgacggtt ccgccagcgc atgtggtagc gcaattggca
    20041 agccgcctgc gtggatgatg gcattcaggt acttttcttg cagagtctgg gtcgcatgac
    20101 ccttaagcct gttcctgcac attacgacac cgataaccgg attgttcatt atattttcca
    20161 tgctcaatct cacaaagtgg actaaattat cgccattact gcgctttaaa tgacctttat
    20221 gttcaatatt ttttcaatct agcagtgggt tttcattttt gcaaactcaa tttaacattt
    20281 gacaaacatt tagtttgcat acagattcga atggtggtca ttatatttta cgctttgata
    20341 acgagcggaa aacaaaccaa aggcgaagaa tcatggaaac caatatcgtt gaagtagaga
    20401 actttgttca gcagtcagaa gagaggcggg gtagcgcctt tacgcaggaa gtgaaacgct
    20461 acctggagcg ctacccgaat acgcaatatg ttgatgtttt acttactgat ttgaatggtt
    20521 gcttccgtgg aaaacgtatc cctgtttcaa gcctgaagaa gctcgagaaa gggtgttatt
    20581 tcccggcctc ggtatttgca atggatattc tgggcaacgt agtagaagaa gcaggtctgg
    20641 gtcaggaaat gggcgagccg gatcgtacct gtgttcctgt tctcggttcc ttaactcctt
    20701 ctgccgccga tccagagttt atcggtcaga tgctcctgac catggtcgat gaagatggcg
    20761 ctccctttga cgttgagccg cggaacgttc ttaaccgcct gtggcagcag ctgcgccagc
    20821 gcgacgctga agggaatctg caaccgccct gcgcgcctgg caccgatgac cgcaatacac
    20881 aaagccaggt ttactccgtt gataacctca accacttcgc cgacgtgctc aatgatattg
    20941 atgaactggc gcagttgcag ctgattccgg cagatggcgc ggtcgctgag gcctcgccgg
    21001 gtcagtttga aatcaacctt taccatactg ataacgtgct ggaagcctgc gatgatgcgc
    21061 tggcactaaa acgcctggtg cgtctgatgg caggaaagca taagatgcac gccactttta
    21121 tggcgaagcc gtatgaagag cacgcgggca gcgggatgca tatccatatc agtatgcaaa
    21181 ataatcgtgg cgagaatgtg ctttctgaca cagaaggcga agattcgccg ctgctgaaaa
    21241 agatgctcgc cgggatgatt gacctgatgc cgtcgtcgat ggcgttgctg gcaccaaacg
    21301 tgaactcgta tcgccgcttc cagccgggaa tgtatgtgcc gacgcaggcg tcgtggggcc
    21361 ataacaaccg caccgtcgcc ctgcgtattc cgtgcggcga ccgtcataat caccgcgtgg
    21421 aatatcgcgt ggcgggtgcc gatgccaacc catatctggt gatggcagcg atttttgccg
    21481 gtattttgca tggccttgat aacgagctgc cgttgcagga agaagtcgaa ggcaacgggc
    21541 tggaacagga aggcttaccc ttcccgattc gccagagcga tgccctgggt gagtttatcg
    21601 agaatgatca cctgcgccgc tatttaggca aacggttttg ccatgtgtat cacgcctgta
    21661 aaaatgatga gctgttgcag tttgagcgcc tcatcacaga aacagaaatt gagtggatgt
    21721 tgaaaaacgc gtaattcccc ttgcggggtt gtttcatcgg cctgagcgga gccaggcctg
    21781 acatttgctg gtaatccaga catttcccgg atgtcgcgtt atggagcgca ggcggcaacg
    21841 ggcttccgtt aacgattaag actgtgacga ggaaatgaga tgatgccgat gattaaatcc
    21901 ccgcatggcg agggaggctg cgtatgcgcc cctcctgcta ctgactggac tccgccccct
    21961 ttgcttccct tgctaaacag gtttgatttc aggtcgacac gaccgcaaac cttattacgc
    22021 aggggaggca gcaattatgg ctattaattc accactgaat attgctgcgc aacccggcaa
    22081 aacccgtctg cgaaaatcac tgaaattgtg gcaggtggta atgatgggtc tggcctatct
    22141 cacgccgatg accgtatttg atacttttgg tattgtctcc ggcattagcg acggtcacgt
    22201 tccggcgtcc tatttgctgg cgctggcggg cgtgctgttt accgctatca gctacggcaa
    22261 actggttcgc cagtttccgg aggccggttc ggcctatacc tacgcgcaaa agtcgattaa
    22321 cccgcacgtc ggatttatgg tcggctggtc atcactgctg gattatctct ttttgccaat
    22381 gatcaacgtt ttgttggcga aaatctatct ctccgccctc ttcccggaag tgccgccgtg
    22441 ggtgtgggtg gtaaccttcg tcgccatttt aaccgccgcg aatctgaaga gcgtcaacct
    22501 ggtcgctaac ttcaataccc tgtttgtact ggtgcaaatc tccatcatgg tggtgtttat
    22561 cttcctggtg gttcagggac tgcataaagg agaaggcgtt ggcaccgtct ggtcacttca
    22621 gccgtttatc agcgagaacg cgcacctgat cccgattatt accggtgcga cgattgtctg
    22681 tttctcgttc ctcggtttcg atgcggtgac cacgctttcg gaagagacgc cggacgccgc
    22741 acgcgtgatc ccgaaagcca tcttcctgac ggcggtatac ggcggcgtta tctttatcgc
    22801 ggcgtcgttc tttatgcagc tgttctttcc cgatatcagc cgctttaaag acccggacgc
    22861 cgcactgcct gaaattgcgc tgtacgtcgg cggcaagctc tttcagtcga ttttcctctg
    22921 caccacgttt gtgaacacat tagcgtctgg cctggcttca catgccagcg tgtcgcgtct
    22981 gctgtatgtg atggggcgtg acaatgtgtt tccggagcgc gtgtttggct atgtgcaccc
    23041 aaaatggcgg actccggcac tcaacgtcat tatggtcggg attgtcgcgc tgtcggcgct
    23101 gttctttgat ttagtcaccg cgacagcact gattaacttc ggtgctctgg tggcgtttac
    23161 cttcgtgaat ctgtcggtgt ttaatcattt ctggcggcgt aaaggaatga ataaaagctg
    23221 gaaggatcac ttccactatt tgctgatgcc gctggttggc gcgctgacgg tgggtgtgct
    23281 gtgggttaac ctagagtcaa cgtcactgac actcggtctg gtatgggctt cgttaggcgg
    23341 cgcatatttg tggtatctga tccgccgcta tcgcaaagtg ccgctgtacg aaggtgacag
    23401 aacgccggtg agcgaaacgt aatagccgga tgcgcggcgc attatcctgc ctgagggtaa
    23461 cgcgccgcgc cattaactat aaaaagatgt atgacccgga tcacgagtgc tatcatccgc
    23521 ccttatgctt agcgcattaa ccccaggggc aaataatgaa ccacgatatc ccgctaaaat
    23581 attttgatat tgccgatgag tacgcgaccg aatgcgcaga gcctgtcgca gaggctgaac
    23641 gcacaccgct ggcccactac ttccagttac tgctcacccg tttgatgaac aatgaagaga
    23701 tcagcgaaga agcccaacat gaaatggctg ctgaagcggg gattaatccc gtacgcattg
    23761 atgagatcgc agagtttttg aatcaatggg gtaatgagta attatttaat tgcagacgat
    23821 cggtcagtat cgttgtccgg ataaagcgtt cacgccgcat ccggctaaca atgacacgca
    23881 ctgcagcgtg ctttaatggg tttctcacga tcgtaccgct ctgctcctcg ctaagttgtt
    23941 ctgtgagtta tagttataga gaaatcacgg gtacgaatgt tgaacagatg gcgacgccac
    24001 ctgttcaacg accaaccaca cggccctcat tacgccccct gaacacaaac ggtaatggca
    24061 aatgtcatta aatattagta agatagcctc tttacataaa atcccttaat attatgcgcc
    24121 aggtattatc gtctcttttg gtgattgctg gacttgtgag tggtcaggca atcgccgcgc
    24181 ctgaatcccc cccgcatgct gatatccgcg acagcggttt tgtctattgc gtcagcgggc
    24241 aagtcaacac ctttaaccca tccaaagcga gcagtgggtt aattgtcgat acccttgccg
    24301 cccagtttta tgatcgactg ctggatgtcg atccctatac ctatcgcctg atgccggaac
    24361 ttgccgaaag ctgggaagta ctcgacaacg gcgcgaccta tcgcttccac ctgcgtcgcg
    24421 atgttccgtt tcaaaaaacc gactggttta ctcccactcg taaaatgaat gccgacgatg
    24481 tggtgtttac cttccagcga atttttgacc gcaacaaccc gtggcataac gtcaacggca
    24541 gcaacttccc ctacttcgac agcctgcaat ttgccgataa cgtgaaaagc gtccgcaaac
    24601 tggataatca taccgttgag ttccgactcg cccagccgga tgcttctttt ttgtggcacc
    24661 tcgcaaccca ttatgcttcg gtcatgtcgg cagaatatgc ccggaagtta gagaaagaag
    24721 atcgccagga gcaactcgac cgtcaaccgg tcggcaccgg tccgtatcag ttgtcggaat
    24781 accgcgccgg gcaatttatt cgcctacaac gtcatgatga cttctggcgc ggtaaaccgt
    24841 taatgccgca ggtagtggtg gatttaggct ccggcggcac cggacgtctg tcgaaactcc
    24901 tgaccgggga atgcgacgtt ctggcctggc ctgctgccag ccagctatcc attttgcgtg
    24961 acgacccgcg cttgcgttta acgctgcgtc ctgggatgaa cgtcgcctat ctggcattta
    25021 acaccgccaa accgccgcta aataatcccg ctgtccgcca tgcactggca ctggcgatta
    25081 ataaccagcg cctgatgcaa tccatctatt atggtacggc tgaaacggcg gcctctattt
    25141 taccgcgcgc ctcgtgggcc tatgacaacg aggctaaaat tactgaatac aatccggcga
    25201 aatcgcgcga acagttgaag gcgttggggc tggaaaactt aacgctgaaa ctgtgggtgc
    25261 ccacacgttc gcaggcgtgg aaccccagtc cactgaaaac tgccgaactg attcaggcgg
    25321 atatggcgca ggttggcgta aaagtggtga ttgtgccggt agaaggtcgc tttcaggagg
    25381 cgcggttgat ggatatgagc catgatctga cgttatccgg ttgggcgacg gacagtaacg
    25441 acccggacag tttcttccgt cctttactga gctgcgcggc aattcattca cagaccaacc
    25501 tcgcccactg gtgcgatccg aaattcgaca gcgtgttgcg taaggcgctc tcctcgcagc
    25561 agctggcggc gcgtattgaa gcctatgacg aagcgcagag tattctggcg caggaattac
    25621 ctattctgcc gctggcgtcg tcattgcgtt tgcaggccta tcggtacgat atcaaaggtc
    25681 tggtacttag cccgtttggt aacgcctcct ttgctggggt gtatcgcgag aaacaggatg
    25741 aggtgaaaaa accatgatta tcttcacctt acgccgcatt ttgttattga ttgtcacctt
    25801 gttcctgctg accttcgttg gcttcagctt gagctatttc acccctcatg cgccgttaca
    25861 aggcgcgtca ctgtggaatg cctgggtgtt ctggtttaac ggcctgatcc actgggattt
    25921 tggtgtttcc agcattaacg gtcaaccgat tgccgaacag ttaaaagagg tcttcccggc
    25981 gacgatggaa ctgtgcatcc tcgccttcgg ttttgcgctg attgttggca tccccgtggg
    26041 gatgattgcc gggattacgc gccataagtg gcaggataac ttgatcaacg ccatcgcctt
    26101 actgggcttt tcaatcccgg tgttctggct ggcgcttctg ttgacgctgt tttgttcact
    26161 cacgctaggt tggttgcccg tttcagggcg tttcgatctg ctctacgaag tgaaaccgat
    26221 taccggtttt gcgttgattg atgcctggct ttcggactca ccatggcggg atgaaatgat
    26281 catgagcgca atccgccata tgatattgcc cgtgatcacc ctgtcggttg cgccaacaac
    26341 tgaagtgatc cgcctgatgc gtatcagcac tatcgaagtg tatgaccaga actatgtaaa
    26401 agcggcggcg acccgcggtt tgtcgcgctt tactattttg cgtcgccacg ttctgcataa
    26461 cgcgttaccg ccggttattc ctcgcctggg cttacagttt tccaccatgc tgacgctggc
    26521 gatgatcacc gaaatggttt ttagctggcc gggtttaggg cgctggttaa ttaacgccat
    26581 ccgccagcag gattacgcag ccatttccgc cggagtgatg gtgtgtggct cactggtcat
    26641 tattgttaac gtgatttctg atattttggg tgccatggct aaccctctga aacataagga
    26701 atggtatgcc ttacgatagc gtatacagcg aaaagcgccc gccgggcacg ctgcgtaccg
    26761 cctggcgcaa attttatagt gacgcctctg cgatggtcgg cctgtacggc tgcgcggggc
    26821 tggctgtact gtgtattttt ggcggctggt ttgcgcctta cggcatcgac cagcaatttc
    26881 tcggttatca attattgccg ccatcatggt cgcgctatgg cgaagtttct ttcttcctgg
    26941 ggactgacga cctcggacgc gatgttttaa gccgtttact gagcggagct gcgccaaccg
    27001 tgggtggcgc atttgtggtg acgcttgccg cgacgatctg tggcctggta ctgggcacct
    27061 tcgccggggc gacacacggc ctccgctcag cggtgcttaa ccatattctg gataccttac
    27121 tggcgatccc ttcgctgctg ttggcaatta tcgttgtagc gtttgccgga ccgagtttgt
    27181 cccacgctat gtttgctgtc tggctggcat tgctgccgcg tatggtacgt tcgatttaca
    27241 gcatggtgca tgacgaactg gaaaaagagt acgttatcgc cgcccgtctg gatggcgcat
    27301 caacgctgaa tattctctgg tttgctgtga tgccaaacat caccgccggg ctggtcaccg
    27361 agatcacccg cgcactgtcg atggcaattc tcgatatcgc cgcgctgggc tttctcgatc
    27421 tcggcgcaca actcccctcg cctgaatggg gagcgatgct cggtgatgcg ctggaactga
    27481 tttatgtcgc cccgtggact gtcatgctgc caggtgcggc aattatgatt agcgtgttgt
    27541 tggttaacct ccttggtgat ggcgttcgtc gtgcgattat tgcgggggtg gaataatgcc
    27601 attactcgat attcgtaacc tgaccattga atttaaaacc ggtgatgagt gggttaaagc
    27661 cgtcgaccgg gtaagtatga ccttaaccga aggtgaaatc cgcggtcttg ttggtgaatc
    27721 cggttccggc aagagtttga ttgcgaaagc gatttgtggg gtgaataaag ataactggcg
    27781 tgttactgct gaccgtatgc gttttgatga tatcgatttg ctgcgtctct ccgcacgcga
    27841 acggcgcaaa ctggttggcc ataacgtgtc gatgattttc caggaaccgc agtcgtgtct
    27901 tgacccttca gaacgtgtgg gtcgccagtt gatgcaaaac atcccagcct ggacctataa
    27961 aggccgttgg tggcagcgtt ttggctggcg caaacgccgt gccattgaac tgctgcaccg
    28021 cgtggggatt aaagatcaca aagatgcgat gcgcagtttt ccctatgagt tgaccgaagg
    28081 tgaatgtcag aaagtgatga tagccattgc actggcgaat caaccgcgtc tgctgattgc
    28141 tgacgaaccg accaactcaa tggagccaac aacccaggcg caaatctttc gcctgctgac
    28201 gcgtctcaac caaaacagca ataccactat tttgcttatc agccatgact tacaaatgct
    28261 tagccaatgg gcggataaaa ttaacgtgct ttactgcggt caaacagtgg aaaccgcgcc
    28321 gagtaaggag ttggtgacga tgccacatca tccttatacc caggcgctga tccgcgcgat
    28381 accagacttc ggcagcgcga tgccgcataa aagtcgcctc aatacgctgc ccggcgctat
    28441 cccactgctg gaacagttac cgattggttg tcgtctgggg ccacgttgcc cgtatgcaca
    28501 acgagaatgc attgtgacgc cacgtttgac gggggcgaaa aatcatctct atgcctgtca
    28561 tttcccgctg aacatggaga aagagtgaga tgatcgaaac gctgcttgaa gtgcgtaatt
    28621 taagtaaaac gttccgctac cggaccggct ggtttcgtcg tcagaccgta gaagcggtaa
    28681 aacccttgag ctttacgcta cgtgaaggcc agacactggc gattattggc gagaatggtt
    28741 cgggtaaatc cacgctggca aaaatgctgg cgggaatgat agagcctacc agcggcgagt
    28801 tattgattga cgatcatcca ctgcattttg gcgattattc cttccgcagt cagcgtattc
    28861 gcatgatttt tcaggatcct tcgacctcgt tgaatccccg tcaacgtatc tcgcaaattc
    28921 tggattttcc actgcgcctg aacaccgacc tggaaccgga acagcgtcgc aaacagatta
    28981 ttgaaacgat gcgtatggtt gggctgctac cggatcacgt cagttactat ccgcatatgt
    29041 tggcacccgg acaaaaacag cgtctgggtc tggcgcgcgc gctaatattg cgcccaaaag
    29101 tcattattgc cgatgaagcc ctcgcttcac tggatatgtc gatgcgttcg cagttgatta
    29161 atctgatgct ggagttacag gaaaaacagg gcatttcgta tatttatgtt acccagcata
    29221 tcggaatgat gaagcacatt agcgaccagg tgctggtgat gcatcagggc gaggttgtcg
    29281 agcgaggcag caccgcggat gtgctggcat cgccgctgca tgaactcacc aaacggctga
    29341 ttgccgggca ctttggcgaa gcattgacag cggatgcgtg gcgtaaagat cgctaatttt
    29401 ctgaatcctc cttccagatt atccacctca ttctgacaag agattaaatc ttgtcagaat
    29461 gaggtgatgg ataaaataaa atcaaatgcg cgtgatttaa gacgcaatct cactttgcag
    29521 gaacgaaagc tctggcgata tcttcgcagc cgacgttttg gtgatttcaa atttcgccgt
    29581 caacatccgg tggggagcta cattctcgat tttgcttgct gctcggcgcg cgtagtcgtt
    29641 gagctggatg gtgggcagca tgatttggca gttgcctatg ataccaggcg cactagctgg
    29701 cttgagtcgc agggctggac cgtgctgcgt ttctggaata acgagattga ttgtaatgag
    29761 gaggcggtgc cggagattat tctgcaggaa ctgaaccgcc gatcaccctc accctgaaag
    29821 ggcgaggggg ccgaccgagc cgaatagctg ttgtggtgaa aacatggaga cagtgctgga
    29881 gaatattcgg caaggtctga accatcccag ccatcgccat aaaagggtta ggggctgtat
    29941 gagcctgttt gttgctgggg taacaatatt tgcacaatat ggtcccctcg cccctctggg
    30001 gagagggtta gggtaagggg aaaagcgccc accctgccgc agcctgctcc ggtcggacct
    30061 ggcaactata gctactcaca gccaggttga ttataataac cgtttatctg ttcgtactgt
    30121 ttactaaaac gacgaatcgc ctgattttca ggcacaacaa gcatcaacaa taaggattaa
    30181 agctatgggt tttctttccg gtaagcgcat tctggtaacc ggtgttgcca gcaaactatc
    30241 catcgcctac ggtatcgctc aggcgatgca ccgcgaagga gctgaactgg cattcaccta
    30301 ccagaacgac aaactgaaag gccgcgtaga agaatttgcc gctcaattgg gttctgacat
    30361 cgttctgcag tgcgatgttg cagaagatgc cagcatcgac accatgttcg ctgaactggg
    30421 gaaagtttgg ccgaaatttg acggtttcgt acactctatt ggttttgcac ctggcgatca
    30481 gctggatggt gactatgtta acgccgttac ccgtgaaggc ttcaaaattg cccacgacat
    30541 cagctcctac agcttcgttg caatggcaaa agcttgccgc tccatgctga atccgggttc
    30601 tgccctgctg accctttcct accttggcgc tgagcgcgct atcccgaact acaacgttat
    30661 gggtctggca aaagcgtctc tggaagcgaa cgtgcgctat atggcgaacg cgatgggtcc
    30721 ggaaggtgtg cgtgttaacg ccatctctgc tggtccgatc cgtactctgg cggcttccgg
    30781 tatcaaagac ttccgcaaaa tgctggctca ttgcgaagcc gttaccccga ttcgccgtac
    30841 cgttactatt gaagatgtgg gtaactccgc ggcattcctg tgctccgatc tctctgccgg
    30901 tatctccggt gaagtggtcc acgttgacgg cggtttcagc atcgctgcaa tgaacgaact
    30961 cgaattgaaa taatcattct gttggtaaat attgggcggc gttctgccgc ccgttatctc
    31021 tgttatacct ttctgatatt tgttatcgcc gatccgtctt tctccccttc ccgccttgcg
    31081 tcaggataac gatttccttt acgaccaagg agcgcccatg gaacaacgcc acatcaccgg
    31141 caaaagccac tggtatcatg aaacgcaatc cagtactgcg gagtatgacg ttctgcctct
    31201 ggtcccggaa gccgcaaagg tcagcgatcc ctttctgctc gacgtgatcc ttgatgaaga
    31261 aacgctggct cccttccttt catggctggt ccctgcgcgc gttcttgcag tggaattgtt
    31321 ccctgaccag cttaccgtga cccgttcaca gaccttcacc gcttatgaac gcttgtctac
    31381 ggccctgacg gttgctcagg tttgcggcgt ccagcggtta tgtaactact attcggcgcg
    31441 acttacgccg ctccccgggc ctgattcctc cagggaaagc aatcataggt tggcacaaat
    31501 cacgcaatat gcccgccaac tggctagctc gccttctatt atcgacaacc gatcgcgcca
    31561 gcatctgaat gacgtcggtc ttactgcctg ggactgtgtg atcattaacc aaatcattgg
    31621 ttttattggc tttcaggcgc ggaccattgc gacatttcag gcttatctcg ggcacccggt
    31681 acgctggtta cccggtctgg aaatacaaaa ctacgccgac gcgtcactgt ttgctgatga
    31741 atcaatacgc tggcgaagca gctatgaggt ggaaaaacta cctgaagagt acacaaaaag
    31801 ttcaacagca gaactttgcc aactggccga aacactctct ctccacccta tttcactttc
    31861 ccttctcgaa aggttgttaa acagtacacg ggttaataca cagccggata ataagcttgc
    31921 ggcgttgtta tgcgcgcgga taaatggcag tcctgcttgt tttgccgcct gtatggattc
    31981 atcaaatgaa tataaaaaat cagccccctt ctgcgcaagg gcgaaaatga aattaaccaa
    32041 tgggctgacc gtcattctgt tgagcacgct accgttcagg cgatacaatg gctgacccga
    32101 gcacccgatc gctttagcgc cgcccagttc agccctttac tcgaacacga aaaatcatca
    32161 acgcagatta ttaatctgct ggtatggagc gggctgtgtg gctggataaa tcgcttaaaa
    32221 atcgcgttgg gtgagacata ttaaccttgc cgcgtcagac agattcgcgt aaaactgtca
    32281 gccgctctaa tggccaccaa aatagacaat tatgtttcag gacaacccgc tgctagcgca
    32341 gcttaaacag caactgcatt cccagacgcc acgcgctgaa ggggtggtaa aagccacaga
    32401 aaaaggcttt ggcttcctgg aagtcgacgc gcaaaaaagt tatttcattc cgccgccgca
    32461 gatgaaaaaa gtcatgcatg gcgaccgaat tatcgcggtg atccacagtg aaaaagaacg
    32521 tgaatccgca gagccagaag aactggttga accgttcctg actcgtttcg tgggtaaagt
    32581 tcagggcaaa aatgaccgtc tggccatcgt tcctgatcat ccactcttaa aagacgccat
    32641 tccttgccgc gcagcccgtg gcctgaacca cgagtttaaa gaaggcgact gggcggttgc
    32701 cgaaatgcgc cgtcatccgc tgaaaggcga tcgttctttc tatgctgaac tgacacaata
    32761 catcactttt ggtgacgacc actttgtacc gtggtgggtt acccttgcgc gccataatct
    32821 ggaaaaagaa gcaccagacg gcgtcgctac cgaaatgctc gatgaaggtc tggttcgtga
    32881 agatctgacc gcgctggatt ttgtcaccat cgacagtgcc agcacagaag atatggatga
    32941 cgcccttttc gctaaggcgt tgccggatga caaacttcag ctgattgtgg cgattgccga
    33001 tccaaccgcg tggattgctg aaggcagcaa gctggacaaa gccgcgaaaa ttcgcgcatt
    33061 caccaactat ctgcctggct tcaacatccc tatgcttcct cgcgagcttt ctgacgatct
    33121 ctgctcactg cgcgccaatg aagtccgccc ggtactggca tgccgcatga cgctctccgc
    33181 tgatggcacc attgaagata atatcgaatt ctttgccgcc accatcgaat ccaaagcgaa
    33241 gctggtgtat gaccaggttt ctgactggct ggaaaatacc ggtgactggc agcctgaaag
    33301 tgaagcaatt gccgaacaag tccgtttgct agcgcaaatt tgccaacgcc gcggcgagtg
    33361 gcgtcataac cacgcactgg tgtttaaaga tcgcccggat taccgcttta ttctcggtga
    33421 aaaaggtgaa gtgctggata tcgtcgccga gcctcgtcgc attgccaacc gtatcgtcga
    33481 agaagcgatg attgccgcta acatttgtgc ggcccgcgtt ctgcgcgata agctcggttt
    33541 tggcatctat aacgtgcata tgggctttga tccggcgaat gccgacgcgc tggcagcgtt
    33601 gctgaaaacg cacggtctgc atgtcgatgc cgaagaagtg ctcacgctgg acggtttctg
    33661 caaactgcgt cgtgaactgg atgcgcaacc aactggtttc ctcgacagcc gcattcgtcg
    33721 cttccagtca tttgctgaaa ttagcactga acccggtcct cactttggcc tcggtctgga
    33781 agcatacgcc acctggactt cgccgatccg taaatatggc gacatgatca accaccgtct
    33841 gctgaaagcg gttatcaaag gcgaaactgc gacgcgtccg caggatgaaa tcactgtcca
    33901 aatggccgaa cgtcgccgtc tcaaccggat ggcagaacgt gatgttggcg actggttata
    33961 cgcacgcttc ctgaaagata aagccgggac cggcacccgt ttcgcggcgg aaattgtcga
    34021 tatcagccgt ggcggcatgc gtgtgcgttt ggttgataac ggcgctatcg cctttattcc
    34081 ggcacctttc ttacacgctg tgcgcgatga actggtttgc agccaggaaa acggcactgt
    34141 acaaattaaa ggtgaaacgg tttacaaagt aactgacgtt attgacgtca ccattgccga
    34201 agtccgcatg gaaacccgcg gcattattgc gcgcccggtc gcgtaatctc ctttcacggc
    34261 ccattcctca tggatgggcc gtttatttcc ccgctctacc ttcgttatat ccaggcagat
    34321 tttttaactg actttcgttt gaaaactggc gtttttccaa aactggatta caaataaata
    34381 tataaaaaaa caatgcgttt agccgccgtt aatttcatgt aagtcatgtt gttcctttta
    34441 tttaaccgcg gactccgcag ttaaccggag gatatgcatc atgaaaaccg ttagggagtc
    34501 cacaacgttg tacaactttc tcggatcgca caatccatac tggcggttga cggaaagcag
    34561 cgatgttttg cgcttttcta ccaccgaaac cacagaacct gatcgtactt tgcagttatc
    34621 tgccgaacag gctgctcgca tcagggaaat gacggtcatc acctccagcc tgatgatgag
    34681 tctgaccgtc gatgagagcg atctttctgt gcatctggta ggacgaaaaa tcaataaacg
    34741 ggaatgggct ggcaacgcgt ctgcatggca tgacacaccg gcagttgctc gtgatttatc
    34801 acacgggctt tcctttgctg agcaggtagt ttctgaagca cattccgcaa tagtgattct
    34861 cgacagccgg gggaatatcc aacgcttcaa tcggttatgt gaagattaca ctgggttgaa
    34921 agaacacgac gtcattggcc aaagcgtgtt taaactgttt atgagccgtc gtgaagctgc
    34981 ggcatccagg cgcaataacc gtgtattttt tcgaagcggc aatgcatatg aagtcgaact
    35041 gtggatacca acacgtaaag gccagcggct gtttctgttt cgcaataaaa taaatttgtc
    35101 cacagcggca gtggcaaaaa cgagattttt ttaatctgtt ccggtaccga cattaccgaa
    35161 gagcgccgcg ctcaggagcg actgcgtatt ctggcaaata ccgacagtat caccggactg
    35221 ccgaatcgta acgcaatgca ggagttaatc gatcacgcta ttaatcaggc agataacaat
    35281 aaagttgggg ttgtgtatct tgatttggat aattttaaaa aggtcaacga cgcctatggg
    35341 catttgtttg gtgaccagtt attacgcgac gtgtcattgg ctattttaag ctgtctcgaa
    35401 catgaccagg tgttggcgcg tccaggtggc gatgagtttc tggtactggc atccaatacc
    35461 tcacaaagcg cgctggaagc aatggcatca cgaattttga cccgcttacg cctccccttt
    35521 cgtattggtt taattgaagt ttataccagc tgttcagtag gtatttcgct ctctcccgaa
    35581 catggttcgg acagcgcgac tattattcgt cacgccgaca cagcaatgta cacagcgaag
    35641 gaaggcggac gaggacaatt ttgcgttttt actccagaaa tgaatcagcg ggtatttgaa
    35701 tatctctggc tggataccaa cttgcgtaaa gcactggaaa acgatcagtt ggttattcac
    35761 tatcaaccga aaatcacctg gcgtggcgaa gtgcgcagtc tggaagcact agtacgttgg
    35821 cagtcacctg aacgtgggtt gattccaccg ttggacttca tttcctacgc cgaagagtca
    35881 gggctaattg tgcctttagg ccgttgggtg attctcgatg tcgtacgcca ggtggcaaag
    35941 tggcgggata aaggcataaa cctgcgagtg gcggtaaata tttctgcacg tcagctcgcc
    36001 gatcaaacca ttttcaccgc cctgaaacag gttctccagg aactcaattt tgaatactgc
    36061 cctatagatg ttgaactgac agagagttgt ctgattgaga atgatgaact ggcactgtct
    36121 gttattcaac aatttagcca actaggtgcg caagtgcatc tggatgattt tggtaccggc
    36181 tactcttcac tttcgcaact ggcgcgcttt ccgatcgatg ccatcaaact tgaccaggtt
    36241 tttgttcgag atattcacaa acaacctgtc tcgcagtcac tggtccgggc gatcgtcgct
    36301 gtggcccagg cattgaatct tcaggtgatc gccgaaggtg tagagagtgc aaaggaagat
    36361 gcttttttaa ccaagaacgg gatcaatgag cggcaaggat ttttgtttgc caaaccgatg
    36421 cccgccgtcg ccttcgaacg ctggtataaa cgctatctga agcgcgcata agtagatgaa
    36481 tatatatact tagcgctata tcccgtcatt atatatcgta ttcctggatg ataattagcc
    36541 gttgctgtta aacacttctc ttttagcggc aatttttgca ttattcattt tccacttttg
    36601 ctataaggat gtccttgatg tctgaatttg atgcccagcg cgttgccgaa cgtattgata
    36661 ttgtgttaga tatccttgtg gcaggcgatt accactctgc gatccataat ctcgagatac
    36721 ttaaagcaga actcttgcgc caggttgctg aatctacgcc agacatcccg aaggcaccgt
    36781 gggagattta acaatcgtgc cttcaggttt tcctatttga ggatgcccgg cgcgtcagta
    36841 tttttttatt tagtattata acgttataag aattacagcg atgaattccc gacaacaaac
    36901 tattctacag atggtcattg accagggtca ggttagcgta accgatctgg caaaagccac
    36961 tggagtttct gaagttacca ttcgccagga tctcaacacc ctcgaaaaac tgagttacct
    37021 ccgccgtgca catggctttg cagtttcgct tgatagtgat gacgtcgaaa cccgtatgat
    37081 gagcaactat acgctgaagc gtgaactcgc cgagtttgcc gcgtcactgg ttcaaccggg
    37141 cgaaaccatc tttatcgaaa atggcagcag caatgccctg ctcgcccgga ctctggggga
    37201 gcagaagaaa aatgtcacta tcatcacggt cagcagctac atcgcgcatt tgctgaaaga
    37261 cgcgccttgt gaagtcattt tgctcggtgg cgtgtaccag aaaaaaagcg aaagtatggt
    37321 tggcccgttg acacgccagt gcatccaaca ggtgcatttc agcaaagcat ttattggtat
    37381 tgatggctgg caacctgaaa ctggattcac cggtcgcgat atgatgcgta ccgatgtggt
    37441 caatgccgtg ctggaaaaag agtgcgaagc gatagtcctg actgacagct cgaaatttgg
    37501 tgctgtacat tcatactcca tcggtcccgt tgagcgattc aatcgcgtga ttaccgattc
    37561 gaaaatacgc gccagcgatc tgatgcatct tgagcacagt aaactcaccg ttcacgtcgt
    37621 tgacatttaa ccatttccct tctctacgga tgatttgcag tttggcaaat catccgctct
    37681 aagatgattc ctggttgata attaagacta tttacctgtt attaacacgc ttaagatata
    37741 aaattattat cagcgatata acaggaagtc attatcacct gcgtgatata accctgcgcg
    37801 cgagcagatt tcacggaata atttcaccag gcttattctt agctattata gttatagaga
    37861 gcttacttcc gtgaatcata aattcaggag agagtattat gtttgtaacg agcaaaaaaa
    37921 tgaccgcggc tgttctggca attactttgg caatgtctct gagtgcctgt tctaactggt
    37981 ctaaacggga ccgcaacacc gcaatcggcg cgggtgcagg ggcattaggc ggtgcagtac
    38041 tgaccgatgg cagtacgttg ggtacattag gtggtgcagc tgtcggtggt gttattggtc
    38101 accaggtcgg taaataatcg taaataaatg tcgattatcg actcgcaatt ataatacgct
    38161 tgattctgtt cagtacgaaa taactgaata atacgcacag taaaatattc gaaagccacg
    38221 gatatatccg tggcttttta tgttaaccgc ctgcgagttt taccttcatc cctttcgctt
    38281 caagtagtga ttttaataaa tcacgcttat cgccctggat ttcaataact ccatctttaa
    38341 ctgctccgcc gcagccgcat tttttcttca gttccgctgc aagttttgtc agttcagcat
    38401 catcgagatc gacaccggta atcaggcaaa cgcccttacc tttacgtcca ctggtctgac
    38461 gctgaatacg caccacaccg tcgcctttag ggcgtacagg ggccgctttg ggctcatcaa
    38521 tacgtccggt ttccgttgag tagaccagac ggctgttgga atcactcatc atgcactccg
    38581 ctgtaaagag gcgttgatcg ctttcagcgt ctgcgctgga tctaccgatt gcgttaccgg
    38641 gcgaccaatc accatataat caacaccagc cgacaacgcc tgttctggcg tcataatgcg
    38701 gcgctggtca ccagcttcac tcccctgcgg acgaatgccc ggcgtaacca gtttgaactc
    38761 ctgaccgaat acctgtttaa agcgcacagc ttcctgagca gaacacacca caccatcaag
    38821 gccacatttt tgcgtcagtg ccgccagacg ttctgcataa tctgcaggtg acagtgtcat
    38881 gccaagatcg accaggtcgc tggcttccat gctggtcaac actgtcacag caatcaaaag
    38941 cggtgcatct ttgccaaacg gaaccagtgc ctcacgcgct gcggtcatca tacgcgcccc
    39001 accagaggca tgaacattca ccatccacac gcctaagtca gctgcagcag cgacagcgtg
    39061 cgctgcagtg ttaggaatat cgtggaattt caggtcaaga aagatatcaa aaccacgctg
    39121 ttgaagttcg cgcacaaact gtggcccaaa caatgtaaac atctctttgc cgaccttcag
    39181 acgacaatcg cgtgggtcga tcttgtcgac aaaggacagc gcgtcatcac gattatgata
    39241 atcaagggca acaaccacag gagaattcgt aacagcgcgg gaagaagatg aagcagttaa
    39301 cgtcatgacc agaccttctt gatgatgggc accggaagtg gcgcgaaaca ggtaaacggc
    39361 gagcattcta cccgtggaaa taaaaaatta acagttgcga tttttcccct ggcaggcttt
    39421 ttctgctccg tcgttgccgg agcacaatgg aacatcaata actagtatgt tgtaactaaa
    39481 gtcggatttt tttaaaaatt acaggccatc aagaccgcga atcggtttaa tggttgacca
    39541 ggcccgacaa gacggacaat gccagtagag ggtgtatgcg gtaaaaccac atttctggca
    39601 gcgataacga ggcttactac gtaccttttc gccaaccatg tcacgcagca ccatcaggct
    39661 ctctttggca cgcccttctt ccgcttcatt taagtggtaa tccattaact tatggaacac
    39721 acgcatggtc ggatgacgct gaagctggcg cgtaatatag acctgtgcgg cctcactacc
    39781 gtcgcgcgct tcgatgatat cagcaagcat caattcagca tcggcaccgg tgttctcttc
    39841 caccgcgcgt tgcaggaatt ctgcccattc ggcagtttta cccaactgct gatagcaggt
    39901 ttgcaacatt tccagcgttt cgctgaccag ttctctgtcc tgggatatca cgcgttgcaa
    39961 actttcgacg gctttggcgt attctccttt cgccataaac acgcgtccca tcatgatgga
    40021 tacgcgggcg ctgtttttat ctgccgccgc cccttttttt agcaaggtca tggcacgatc
    40081 gagatcgtcg ctggccatat gctgcagggc taactcacag tagaaatggg caatttcgac
    40141 gcgctgttta tctttaccca gcttcaccag gcgttcggca acatcaattg ctttctgcca
    40201 ctcgctggta gcctggtaga tttgtagcaa ctgttgcagc gcgccaatgc ggaagtcagt
    40261 ttcatcggtc agctgattga acatgtcttc cgcgcggtcg tataacccgg cggccatgta
    40321 atcacgcccc agttgttgaa tcgccaacag acgctgttca taggtcagtg aggcgctttc
    40381 cattagggtc tgatggatgc gaatagcgcg atcaacttcg ccacgcgaac ggaacaggtt
    40441 tccgagcgta aggtgggctt caacggtgcc tgtatcctct ttaagcatat cgagaaacag
    40501 gtctaccgct ttatcctgtt gattactaag caggaagtta acccccgcta cgtaatcacg
    40561 cgacaagcgg ttggcttcat cttgcttgtt ttgttgcgca cttctgcggc ccatatacca
    40621 gccataggca gcggctacag gcaaaagcag aaacaacaac tccagcatag aaagttattc
    40681 cttcgccgct gacgagtgcg gcactacagc cacgtcagtc gcgggtgaaa gctggttttc
    40741 cagtcgcttt attttacgtt cagcgcgcgc cagggaaaca cgaactcgca gccagaacag
    40801 gccacaaatc aaccaaccga tagcaacccc cgcagcaaac aataccgcca gcaatgtgga
    40861 aatacggtac tccccttgcg ctaacagata attaaacgtc acctgttgat cgttctgcgc
    40921 acccaacgtg accgaaatca cgaagatcgc taacaccagt aagaaaatga gtaaatattt
    40981 cacattactt cccgttatgt ggttaaagcg aataaattat ggtcaactta ccgcaatagc
    41041 cctttaaagt accattttcg cgccgtctgc gaaatggcat ccacgcgcat tgttgatgat
    41101 ttaagggcta aattcggaaa atcaaccagc gttaactttc ttgttctcgt tgcgctattt
    41161 cgcgattttc ttccgcaggt ggtgttaatg gcccacaaat tcgttgcgca agccacgttg
    41221 ccaccgccac cagcgcccac gaaatcaacg tagctactac cagatcgcgt ggccaatgca
    41281 tcccgagcag caggcggctt cccatgactc ccgttgccca gaccagcaag atagcaatgg
    41341 ttaacgttcg ccgacgcggc cacagcaaac caacggccag cagtgcccaa ctggcagcaa
    41401 acatcgtgtg accggaagga aaggcaaacc ccgtctcttt ctgccagtgt gaacgcaaat
    41461 attgtgggat atttttctct tcagccaact gttctttcac tagatttccg cgttctgctc
    41521 gctttaaagt gtagaactca tcaaccggaa tatgatgtgt tttttccagc cagataacaa
    41581 aaggtcgtgg ttcctggact ttgtctttga tccaggattt aacgccttgt cccacaatga
    41641 ttacggccgc cagaatggca aataatacaa aggcagcctt aatgcgaaaa cgcagacacc
    41701 agagaaacca gccgaataaa atcaaatgtg taatgacgcc ccagggctgg gtgacagttt
    41761 cagtaaccca aaaagccgct tttagtagcc aactttgttc tccaggttgc caacgccagc
    41821 cagaaatcca tacggctact ggcatgacaa gcaatagtgc agctcccact gcggtacgtc
    41881 tggcaatcga acgcatggcc tctccttttt gataagtccc acaatcataa ctgaaaacgc
    41941 cagttccagg aaaaattgac agatttgtgc cattccgtga acgatcgacg cgtcgtgatt
    42001 aggtgaaccc cttctcgtta tggcaaaata agccaataca gaaccagcat tatctggaga
    42061 atttcatgca gcttaaacgt gtggcagaag ccaaactgcc aaccccatgg ggcgatttcc
    42121 tgatggtggg atttgaagaa ctggcaaccg gacacgatca tgtcgcgcta gtctatggcg
    42181 atatttccgg gcataccccg gtacttgcgc gcgtccattc cgaatgtctg accggtgacg
    42241 ccctgttcag cttgcgctgc gattgtggct tccagctcga agcggcattg acgcaaattg
    42301 ccgaggaagg ccgtggtatt ttgctgtatc accgtcagga aggtcgtaac attggtctgc
    42361 tgaataaaat ccgcgcttac gcactgcagg atcaaggtta cgataccgta gaggctaacc
    42421 atcagttagg cttcgccgct gatgagcgcg acttcactct ttgcgctgat atgttcaaac
    42481 tccttggcgt caatgaagtc cgcttgttaa ccaataaccc gaaaaaagtc gaaattctga
    42541 ccgaagcagg gattaatatt gttgaacgcg taccattgat tgtaggtcgt aaccccaata
    42601 acgaacatta tctcaatacc aaagccgaga aaatgggtca tttgctgaac aaataaccct
    42661 ctcgcattgt ggaattaatt tgcttgccgg aagcaaaata accggcaagc aaatatttgt
    42721 tacttcagca tattacgaat gacataatgc aaaatgccgt cgttctggta gtaggtcaac
    42781 tccgtcgcgg tgtcgatacg acaacggcag ggtacgacct cctggctacc atccgcgcgc
    42841 gtaagcgtca ccggaaccgt cgcaccgggt tgtagatttt gcagatcgcc aatatcaatc
    42901 ttctcttccc cggttagtcc taacgtttta cgcgttacgc cttgcggaaa ttccagcggc
    42961 aggatgccca tgccaattaa attcgaacgg tgaattcgtt caaacgattc ggcaatcacc
    43021 acacgaattc caagcagacg aggacctttt gccgcccagt cacggcttga gcctgaacca
    43081 tactctttcc cggcaatcac cgccagcggc gtttgttcct gcttatagcg catcgcagca
    43141 tcataaatag agactacgtc gctgtcaggt aaatgccgcg tcatcccccc ttcaacgcca
    43201 ggcaccattt cattacggat gcgaatattg gcgaaggtgc cgcgcatcat cacttcatgg
    43261 ttaccacgcc gcgaaccgta ggagttaaag tcttttcgct caacaccccg accttgtaga
    43321 tatcgacccg ctgggctgtc gggcttaata ctgcccgccg gagagatatg gtcagtggtg
    43381 actgaatccc ccagcattgc gaggatccgc gcaccgtgaa tatcttccac tggtgctggt
    43441 gttgcctgca tttcatcaaa gaaaggcgat aagcgaatat aggttgagtc ctcctgccaa
    43501 ccgtaggtat cggatcgtgt gacgttaatt tccttccact ctgctgtgcc ttcaaaaact
    43561 tctgcgtact ctttgcggaa catttctgtg gagacttgtt ctaccgcacg ggcaatttct
    43621 tgtgccgatg gccagatatc tttcagataa accggatcgc ctttacgatc atgaccaata
    43681 ggctcagaag ccaggttgat attcatattt cccgccagcg cataggcaac caccagcggc
    43741 ggcgaggcca gccagttggt tttaaccagc ggatggatac ggccttcaaa gttacggttg
    43801 ccggacagca ctgcaccgac ggttaaatcg ccttttttga ttgccgtttc gataggatcg
    43861 ggcagcggcc cagagttacc aatacaggta gtacaaccgt atcccacaag gttaaacccc
    43921 agttcgtcga gaaacggtgt cagtttcgct tttgccagat agtcagaaac aactttcgag
    43981 cctggtgcca gcgacgcttt gacccacggt tgccgcttga ggccaagaat tacggctttt
    44041 ttcgccagca agcctgcagc catcagcaca cttgggttag aggtgttggt gcacgaggtt
    44101 atcgcagcaa tgaccacagc gccatcaggt aactgatact gatgtccgtt cataacataa
    44161 tcgaccggct ggcgatcttt atgcgtggca ttcacttcca gttcgttact ggcggcaaat
    44221 acttttggta catcgggcag tgcaacgcga tcctgtgggc gttttggccc tgccaggctc
    44281 gcttcaacgt cattcatatc cagttctaac gtactggtaa aaatcggttc atcgcccggg
    44341 ttacgccaca tgccctgcgc tttggcatat ttttcgacca actcaacctg atcttcgctg
    44401 cgcccgctta aacgcatgta atcgagggtt acagcatcga ttgggaagaa gccacaggtg
    44461 gcaccatatt ctggcgacat attggcaatg gtggcgcgat ccgccagcgg tagtgaatcc
    44521 agaccatcac cataaaattc gacgaatttc tccaccacgc catgtttgcg cagcatttgg
    44581 gtaacagtga gaaccaggtc tgtggcggta ataccttcac gtaatttacc ggtaagttta
    44641 aagcccacta cgtccgggat aagcatggaa accggctggc ctaacattgc ggcttct
//
LOCUS       DAZPDU010000012        43267 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZPDU010000012 DAZPDU010000000
VERSION     DAZPDU010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43267
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.012"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            48..566
                     /locus_tag="ACHURS_000605"
     CDS             48..566
                     /locus_tag="ACHURS_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFR1236417.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            582..1121
                     /locus_tag="ACHURS_000606"
     CDS             582..1121
                     /locus_tag="ACHURS_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFR1236418.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLTLAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1216..2793)
                     /gene="guaA"
                     /locus_tag="ACHURS_000607"
     CDS             complement(1216..2793)
                     /gene="guaA"
                     /locus_tag="ACHURS_000607"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFR1236419.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWARLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2862..4328)
                     /gene="guaB"
                     /locus_tag="ACHURS_000608"
     CDS             complement(2862..4328)
                     /gene="guaB"
                     /locus_tag="ACHURS_000608"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFR1236420.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4490..5860
                     /gene="xseA"
                     /locus_tag="ACHURS_000609"
     CDS             4490..5860
                     /gene="xseA"
                     /locus_tag="ACHURS_000609"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFR1236421.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5857..6072)
                     /locus_tag="ACHURS_000610"
     CDS             complement(5857..6072)
                     /locus_tag="ACHURS_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFR1236422.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6141..7613)
                     /gene="der"
                     /locus_tag="ACHURS_000611"
     CDS             complement(6141..7613)
                     /gene="der"
                     /locus_tag="ACHURS_000611"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFR1236423.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLERVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDTNVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7731..8909)
                     /gene="bamB"
                     /locus_tag="ACHURS_000612"
     CDS             complement(7731..8909)
                     /gene="bamB"
                     /locus_tag="ACHURS_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFR1236424.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8920..9540)
                     /gene="yfgM"
                     /locus_tag="ACHURS_000613"
     CDS             complement(8920..9540)
                     /gene="yfgM"
                     /locus_tag="ACHURS_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFR1236425.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGKALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9558..10832)
                     /gene="hisS"
                     /locus_tag="ACHURS_000614"
     CDS             complement(9558..10832)
                     /gene="hisS"
                     /locus_tag="ACHURS_000614"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFR1236426.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10943..12061)
                     /gene="ispG"
                     /locus_tag="ACHURS_000615"
                     /gene_synonym="gcpE"
     CDS             complement(10943..12061)
                     /gene="ispG"
                     /locus_tag="ACHURS_000615"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFR1236427.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12088..13101)
                     /gene="rodZ"
                     /locus_tag="ACHURS_000616"
     CDS             complement(12088..13101)
                     /gene="rodZ"
                     /locus_tag="ACHURS_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFR1236428.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13386..14540)
                     /locus_tag="ACHURS_000617"
     CDS             complement(13386..14540)
                     /locus_tag="ACHURS_000617"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFR1236429.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14690..15121)
                     /gene="ndk"
                     /locus_tag="ACHURS_000618"
     CDS             complement(14690..15121)
                     /gene="ndk"
                     /locus_tag="ACHURS_000618"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFR1236430.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15270..17582)
                     /gene="pbpC"
                     /locus_tag="ACHURS_000619"
     CDS             complement(15270..17582)
                     /gene="pbpC"
                     /locus_tag="ACHURS_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFR1236431.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAAQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQEG
                     INGIRFPIWLDDKGQRVAADCPQAKQEMVNVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(17583..22544)
                     /gene="yfhM"
                     /locus_tag="ACHURS_000620"
     CDS             complement(17583..22544)
                     /gene="yfhM"
                     /locus_tag="ACHURS_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFR1236432.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRNVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGLRPQFALK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLQADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LSVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGIALKTMGDATRGEEAIALALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKAQNSNLNSDQLVTLQVSNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     VGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            22751..23596
                     /gene="sseA"
                     /locus_tag="ACHURS_000621"
     CDS             22751..23596
                     /gene="sseA"
                     /locus_tag="ACHURS_000621"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFR1236433.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            23932..24057
                     /gene="timP"
                     /locus_tag="ACHURS_000622"
     CDS             23932..24057
                     /gene="timP"
                     /locus_tag="ACHURS_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFR1236434.1"
                     /translation="MKIRCFCIVLIVSGALFSQVNNNRSLSGDNLLVVNNLQSSK"
     gene            complement(24089..24865)
                     /gene="sseB"
                     /locus_tag="ACHURS_000623"
     CDS             complement(24089..24865)
                     /gene="sseB"
                     /locus_tag="ACHURS_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFR1236435.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(25007..26290)
                     /gene="pepB"
                     /locus_tag="ACHURS_000624"
     CDS             complement(25007..26290)
                     /gene="pepB"
                     /locus_tag="ACHURS_000624"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFR1236436.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(26468..26668)
                     /gene="iscX"
                     /locus_tag="ACHURS_000625"
     CDS             complement(26468..26668)
                     /gene="iscX"
                     /locus_tag="ACHURS_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFR1236437.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(26680..27015)
                     /gene="fdx"
                     /locus_tag="ACHURS_000626"
     CDS             complement(26680..27015)
                     /gene="fdx"
                     /locus_tag="ACHURS_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFR1236438.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(27017..28867)
                     /gene="hscA"
                     /locus_tag="ACHURS_000627"
     CDS             complement(27017..28867)
                     /gene="hscA"
                     /locus_tag="ACHURS_000627"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFR1236439.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(28884..29399)
                     /gene="hscB"
                     /locus_tag="ACHURS_000628"
     CDS             complement(28884..29399)
                     /gene="hscB"
                     /locus_tag="ACHURS_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFR1236440.1"
                     /translation="MDYFTFFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(29495..29818)
                     /gene="iscA"
                     /locus_tag="ACHURS_000629"
     CDS             complement(29495..29818)
                     /gene="iscA"
                     /locus_tag="ACHURS_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFR1236441.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(29835..30221)
                     /gene="iscU"
                     /locus_tag="ACHURS_000630"
     CDS             complement(29835..30221)
                     /gene="iscU"
                     /locus_tag="ACHURS_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFR1236442.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(30249..31463)
                     /gene="iscS"
                     /locus_tag="ACHURS_000631"
     CDS             complement(30249..31463)
                     /gene="iscS"
                     /locus_tag="ACHURS_000631"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFR1236443.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(31704..32192)
                     /gene="iscR"
                     /locus_tag="ACHURS_000632"
     CDS             complement(31704..32192)
                     /gene="iscR"
                     /locus_tag="ACHURS_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFR1236444.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRIQDAIDVKL
                     RA"
     gene            complement(32493..33230)
                     /gene="trmJ"
                     /locus_tag="ACHURS_000633"
     CDS             complement(32493..33230)
                     /gene="trmJ"
                     /locus_tag="ACHURS_000633"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFR1236445.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            33349..34152
                     /gene="suhB"
                     /locus_tag="ACHURS_000634"
     CDS             33349..34152
                     /gene="suhB"
                     /locus_tag="ACHURS_000634"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFR1236446.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYSQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            34297..35151
                     /locus_tag="ACHURS_000635"
     CDS             34297..35151
                     /locus_tag="ACHURS_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR1236447.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQDDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKETKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            35342..36622
                     /gene="csiE"
                     /locus_tag="ACHURS_000636"
     CDS             35342..36622
                     /gene="csiE"
                     /locus_tag="ACHURS_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFR1236448.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(36614..37753)
                     /locus_tag="ACHURS_000637"
     CDS             complement(36614..37753)
                     /locus_tag="ACHURS_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFR1236449.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     SAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAVRQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(37913..38803)
                     /gene="hcaR"
                     /locus_tag="ACHURS_000638"
     CDS             complement(37913..38803)
                     /gene="hcaR"
                     /locus_tag="ACHURS_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFR1236450.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            38939..40300
                     /gene="hcaE"
                     /locus_tag="ACHURS_000639"
     CDS             38939..40300
                     /gene="hcaE"
                     /locus_tag="ACHURS_000639"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFR1236451.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            40297..40815
                     /gene="hcaF"
                     /locus_tag="ACHURS_000640"
     CDS             40297..40815
                     /gene="hcaF"
                     /locus_tag="ACHURS_000640"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFR1236452.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLEQLDKEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            40815..41135
                     /locus_tag="ACHURS_000641"
     CDS             40815..41135
                     /locus_tag="ACHURS_000641"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFR1236453.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            41132..41944
                     /gene="hcaB"
                     /locus_tag="ACHURS_000642"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             41132..41944
                     /gene="hcaB"
                     /locus_tag="ACHURS_000642"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFR1236454.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAARYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            41954..43156
                     /gene="hcaD"
                     /locus_tag="ACHURS_000643"
     CDS             41954..43156
                     /gene="hcaD"
                     /locus_tag="ACHURS_000643"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFR1236455.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIAIGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
BASE COUNT        10265 a        11949 c        10863 g        10190 t
ORIGIN      
        1 gatgatctta aatgtcttat ttttcgtaat gtgtataaca aggaatagtg atgaaattta
       61 aaaaatgtct tctgcctgtg gcaatgttag cgtcattcac tctggcagga tgccagtcaa
      121 atgctgacga tcatgccgcc gatgtttatc aaaccgatca actgaatacc aaacaagaaa
      181 ctaaaaccgt taatattatt tccattcttc ccgcaaaagt tgccgtagac aactcccaaa
      241 ataaacggaa cgcacaagcc ttcggcgcgc ttattggcgc agtcgctggc ggtgttatcg
      301 gccacaacgt cggttctggc agcaattccg gaacgacggc aggggcagtt ggcggcggag
      361 ctgtaggcgc ggcagcgggt tctatggtga atgataaaac cttagtggaa ggtgtttctt
      421 taacatataa ggaaggcacc aaagtgtata cctccaccca ggtgggtaaa gagtgccagt
      481 ttacgacagg tttagccgtt gttattacca caacgtataa cgaaacgcgt attcagccaa
      541 atactaaatg tcctgaaaag agctaataat caggaggagt catgaagaaa gtttttcttt
      601 gcgccatctt agcctcctta agctatccgg ctatcgcctc atcattgcag gatcaactct
      661 ctgctgtcgc agaagcggaa cagcaaggta aaaatgaaga gcaaaggcag catgacgaat
      721 gggtcgcgga gcgcaacagg gaaatccagc aagagaagca acgtcgcgca aatgcccagg
      781 ccgccgctaa caaaagagcg gcaacggcag cggcaaataa gaaagctcgt caggataaac
      841 tggacgccga agccactgcg gacaaaaaac gcgatcaaag ttatgaagat gagctacgca
      901 gcttagagat tcagaaacaa aaactgacgc tggcgaaaga agaagcccgc gttaagcgag
      961 aaaacgaatt tatcgatcag gaactgaagc acaaagctgc gcaaaccgat gtggtgcaat
     1021 ctgaagctga cgccaacaga aatatgactg aaggcggtcg cgatctgatg aaaagcgtgg
     1081 gcaaagcaga agagaacaaa tcggacagct ggtttaatta atcgatgtta gtaacttcaa
     1141 gcctatgatt cttgaagata aaaaaccctc tgtagtaaca gagggttttg ttcattcata
     1201 gtgcagggat taaaatcatt cccactcaat ggtagctggc ggcttgccgc tgatgtcata
     1261 caccacgcgg gaaataccgt tcacttcatt gataatgcgg ttggaaacgc gaccgaggaa
     1321 atcgtacggc agacgcgccc agtgtgcggt cataaagtcg atggtttcga cagcacgcag
     1381 agagacaacc cagtcatact tacgaccatc gcccattacg ccaacggaac gtaccggcag
     1441 gaacacagtg aacgcctggc tgactttgtc gtacaggtcc gctttacgca gttcttcaat
     1501 gaagatggcg tcagcacggc gcagcaggtc acagtactct ttcttcactt cacccagcac
     1561 acgaacgcca aggcctggtc ccgggaatgg gtgacggtac aacatgtcgt acggcaggcc
     1621 cagctccaga ccaatcttac gcacttcgtc tttgaacagc tctttcagcg gttcaaccag
     1681 gcccatcttc atctctttcg gcaggccgcc cacgttgtgg tgagatttga tgacgtgtgc
     1741 tttaccggtt gcagacgccg cagattcgat aacgtcaggg tagatggtgc cctgcgccag
     1801 ccacttcacg tcttccagtt tcagcgcttc ttcatcgaat acttcaacga aaacgcgacc
     1861 gatgatttta cgttttgctt ccggatcgtt ttcgccagcc agcgctgaca ggaagcgatc
     1921 ttctgccgga acgtgaacaa tgttcagacc aaagtgatcg ccaaacatat ccagaacctg
     1981 ctctgcttcg ttgagacgca gcaggccgtt gtcgacgaat acgcaagtca ggtttttacc
     2041 gatagcgcgg tgcagtagca ttgcggttac ggaggaatcc acgccaccag agaggccgag
     2101 gatgacttta tcgtcgccta cctgctcacg gatgcgggct acagcatcgt cgataatttt
     2161 cgctggcgtc cacagggctt cacactggca gatatcacgc acaaaacgct ccagcatgcg
     2221 cataccctgg cgggtgtgag tcacttccgg gtggaactgt acgccataga agcgtttttc
     2281 ttcgttagcc ataatggcaa acgggcagct ttcggtgctg gctacggtga cgaagtcgga
     2341 cgggatagcg gtaactttat cgccgtggct catccagaca tcgagcagcg gtttaccgtc
     2401 tgcggtcagc gcatcttcga taccgcgaac cagtgcactg tcgtttacga cttcaacctg
     2461 cgcgtagcca aattcacgtt cgttagaggc ttcaacgtga ccgcccaact gcattgccat
     2521 ggtctgcatg ccatagcaaa cgccgaatac cggtacgcct gcttcaaaga catactgcgg
     2581 cgcacgcggg ctgttttctt cagtagtact ttccgggccg ccggaaagaa taatgccgct
     2641 tggattgaag tcacgaattt gtgcttctgt cacatcccac gcccacagtt cgcagtaaac
     2701 acccagctca cgcacgcggc gcgcaaccag ttgagtgtac tgagaaccga agtccagaat
     2761 gaggatgcga tgcttatgaa tgttttccgt cattgacgct tattccgagg caagtgaaac
     2821 agataatata aatcgcccga catgaagtcg ggcgaagaga atcaggagcc cagacggtag
     2881 ttcggggact ctttagtaat ggtcacgtcg tgaacgtggc tttcctgaat gcccgcaccg
     2941 ctgatacgta caaactccgc tttagtacgc agttcgtcga tagtaccaca gccggtcaga
     3001 cccatacagg agcgcaggcc gcccatctgc tggtgaatga tctctttcag gcgaccttta
     3061 taagctacgc gaccttcgat accttccggc accagtttgt cggcagcgtt atcgctctgg
     3121 aaataacggt cagaggaacc tttggacatc gcgcccaggg aacccatacc acggtaagat
     3181 ttgtaagaac ggccctggta gagttcgatt tcacccggag attcttcagt acccgccagc
     3241 atggaaccta ccatcaccgc gcttgcgcca gcggcgatag ctttggcgat gtcgccggag
     3301 aagcgaatac caccatctgc gataaccgga ataccggtgc cttccagggc ttcaactgcg
     3361 tcagcaacgg cagtgatctg cggaacacct acgccagtaa cgatacgagt cgtacagata
     3421 gaaccagggc cgataccgac tttaaccgcg ctgcaaccag cttctgccaa agcgcgtgca
     3481 cctgcagctg ttgccacgtt gccgccgatg atttgcagat ccggatattt agcacgggtt
     3541 tcacggatac gctgcaaaac gccttctgag tgaccgtggg aggagtcgat cagcagaacg
     3601 tcaacgccag cggcaaccag cgcgtcaaca cgctcttcgt tacccgcacc tgcgccaact
     3661 gcagcaccaa cacgcagacg gccttgctcg tctttacagg cgttcggttt acgttccgct
     3721 ttctggaagt ctttcacggt gatcatgccg atcaggtgga attcgtcatc aaccaccagc
     3781 gctttttcaa cgcgtttttc gtgcattttt gccagcacca cttcacgggc ttcaccttca
     3841 cgcacggtga ccagacgctc tttcggcgtc atgtaaacgc taaccggctg gttcaggtcg
     3901 gtaacaaaac gcacgtcacg accggtgata atacccacca gttcgttttc ttcggtaacg
     3961 accggatagc ccgcaaaacc gttacgctcg gtcagttctt tcacttcgcg cagcgtcgtg
     4021 gttggcagaa cagtctgcgg atcagtcacc acaccagatt cgtgtttttt cacacggcga
     4081 acttcttctg cctggcgttc aatggacatg tttttgtgga taaagccgat accgccttcc
     4141 tgagccagag caatagccag gcgcgcttcc gttacggtat ccattgctgc ggaaagcata
     4201 gggatattca gacgaatagt tttcgtcagc tgggtgctga ggtcagcagt attcggcaga
     4261 acggtagaat gagcaggaac gaggagaacg tcgtcaaacg tcagagcttc tttagcgata
     4321 cgtagcatgg gcaatatctc gaccagagtg gttaataaat attgccgcgg cattatacag
     4381 agcgtaaccg attgcatcta cccctttttg caaaaaatgc ttgctatccc cgaagggcgg
     4441 gttactatcg actgaataac ctgctgattt agaatttgat ctcgctcaca tgttaccttc
     4501 tcaatcccct gcaattttta ccgttagtcg cctgaatcaa acggttcgtc tgctgcttga
     4561 gcatgagatg ggacaggttt ggatcagcgg cgaaatctct aatttcacac aaccggcttc
     4621 cggtcactgg tactttacac tcaaagacga caccgcccag gtacgctgcg cgatgttccg
     4681 caacagcaac cgccgggtga ccttccgtcc acagcatgga caacaagttt tagttcgcgc
     4741 caatattacg ctctacgagc cgcgcggtga ctaccagata atcgttgaga gtatgcagcc
     4801 ggccggtgaa gggctgctgc aacagaagta cgaacagctc aaagcgaagt tgcaggctga
     4861 aggtttgttc gatcagcaat acaaaaaacc acttccctcc cctgcgcatt gcgttggtgt
     4921 gatcacctca aaaaccggtg ctgcgctaca tgatattttg catgtgttaa aacgtcgcga
     4981 tccttctcta ccggtgatca tctaccccac cgccgttcag ggcgatgacg caccggggca
     5041 aattgttcgc gccattgaac tggcgaatca gcgcaatgag tgcgacgtgt tgatcgttgg
     5101 gcgcggcggc ggttcgctgg aagatttatg gagttttaac gacgaacgcg tagcgcgggc
     5161 gatttttgcc agccgcattc cggtcgtcag cgccgtcggg catgagacgg atgtgaccat
     5221 tgccgatttt gttgccgatc tgcgtgcgcc aacgccgtct gccgccgctg aagtagtgag
     5281 ccgtaatcag caagagttac tgcgccaggt gcaatcgacc cgtcaacggc tggagatggc
     5341 gatggattat tatctcgcca accgcacgcg tcgctttacg cagatccatc accgattaca
     5401 gcaacagcat ccgcagctcc ggctggcacg ccagcaaacc atgcttgagc gcctgcaaaa
     5461 gcggatgagc tttgcgctgg aaaatcaact taagcgtacc gggcaacagc agcagcggtt
     5521 aacacagcgg ctgaatcagc aaaatccaca gccgaagatt catcgcgcgc aaacgcgcat
     5581 tcagcaactg gaatatcgtt tagcagaaac cctgcgcgta cagcttagcg ccacgcgtga
     5641 acgtttcggt aatgcagtaa cgcacctcga agccgtaagc ccactgtcaa cgctggcgcg
     5701 tggatacagc gttactactg ctactgacgg caatgtactg aaaaaagtga agcaagttaa
     5761 agcgggtgaa atgctaacca cacgtctgga agacggctgg atagaaagtg aagttaaaaa
     5821 catccagcca gtaaaaaaat cgcgtaaaaa ggtgcattaa gccagcacga attcaacgcg
     5881 ttttttcgat atcaagccat gcccgtgctg acagaagtaa tctactgcac cgcaggcttt
     5941 taacacctgc aacggttgat ggcagtctgg acaaagagct ttcatttcaa taatttcccc
     6001 gcaggatggg caacgcgcat ggccgttatc ctgatcaagc acatgctgac attgtggaca
     6061 atgcagttcc atatgaattc ctccggtagc catccctcta cattcataga gggaatggca
     6121 gataaaatac ttacggataa ttatttattt ttcttgatgt gcttcatcag acgcttacgt
     6181 ttacgcatct gggttggcgt cagggtgtta cgcttgttcg catacgggtt ttccccttct
     6241 ttgaactgaa tacgaatcgg cgatcccatt acgtccagcg atttgcggaa gtagttcatc
     6301 aagtagcgct tgtaggaatc aggcaggtct ttcacctgat taccgtgaat caccacaatc
     6361 ggcgggttat aaccaccagc gtgggcatat ttcagcttca cacgacgacc gcgtaccagc
     6421 ggcggttggt gatcttcaac agccatcgtc atgatgcgcg tcagcataga ggtccccaca
     6481 cgacgggtgg agctgtcata cgcttcacgt actgattcaa acaagttacc aacaccactg
     6541 ccgtgcaagg cagagataaa gtgcacacga gcaaaatcga taaagcccag acggaagtcc
     6601 agcgtttctt tcacctgctc tttcacttcc tggctcaggc catcccactt attcaccaca
     6661 atgacaagtg agcgcccact attgagaata aagcccagca gcgagagatc ctgatcggaa
     6721 ataccttcgc gcgcatcaat cactaacatc accacgttgg tatcttcaat ggcctgcaac
     6781 gttttgatta cggagaattt ctctacagca tcggtgattt tgccgcgttt acgtacgcca
     6841 gcggtgtcaa tgagcacata ctcacgtcca tcgcgttcca ttggaatgta gatactgtca
     6901 cgcgtcgtgc caggcatgtc ataaacaaca acgcgctctt caccaagaat acggttagtg
     6961 agtgtagact tacctacgtt cggacgaccc acaatcgcca gtttgatcgg cagactttgc
     7021 gggtcgaagt cgtcttcctc ttcttcttcg ccgttctctt ccgcttcaaa ttgcgcccag
     7081 tattcagcgt cttcgtcgac ttcctcttgc ggtgcgagat cttccatcca cggcagcagc
     7141 acacgctcca gcagacttaa tacgccacga ccgtgagatg cggcgatcgg gtagatttca
     7201 cctaaaccaa gcgagtagaa atcaaccact gcctgatcgg gatccagacc gtcagttttg
     7261 tttgccacca ggaaggtcgg tttttcacga gagcgcagat gtttggcaat cgcttcatct
     7321 gccggcatca agcccgcgcg cgcatccacc ataaacagta cgacgtccgc ttcttcaatc
     7381 gccagcagcg actgttccgc catgcgggtt tctacaccgt cttctgtgcc atcaatcccg
     7441 ccggtatcaa tacagataaa ctcacggcct tcaatttccg cacgaccgta cttacggtca
     7501 cgagtcagac ccgggaaatc cgcaaccagc gcatctcggg tgcgagttag acggttaaat
     7561 aacgtggatt ttcctacgtt agggcgcccg acaagcgcga ccacaggtac catgtttaaa
     7621 gcctcatttt tataaatcat cagacaacgc acgctatatt cgcgtcgttg ttaaaaacag
     7681 gaaaacggcc cctgtccagg agccgttttc aaagtgaacg acagagacga ttaacgtgta
     7741 atagagtaca cggttccgtc ttttgcctgg atcagcagtt tgccgtcagc ggcaaccggt
     7801 tcagtctgga aaccggaact atcaactttt tgctgggcaa cgaaacgacc atcttcgacg
     7861 ttaatccagt gcagataacc ttcactgtca cctaccacca ggttgccatt atacagcacc
     7921 ggcgaagtca gcaggcgatg cagcagatcg ctttgtgtcc acagcgtaac gccgccatca
     7981 atggtcaacg ccatcacccg gtcattttga tcgaccagat agatgcgatt gccgtcgacg
     8041 atgaaatcat tcaccgaacc cagttcgcgt ttccacataa tctgaccact gcgcagatca
     8101 agcgccgtca ggttaccatt ataggccaga gcgaaaacaa cgccgttaac aacgacggga
     8161 gtcgtgtcaa catcgctcag acggtcaatt tcggtagaac cggtcgcctg ggaaatacgc
     8221 tgctgccaaa tcatctggcc ctgttccatc agcactgcgc tgacgcgacc attatcgccc
     8281 cccacgacgg ccgcaccaaa agccgttgcc ggcgcagact cgccacgcaa agagagcgaa
     8341 ggcatatcga ggttaactgt ccatttgaca gcgccgtcag cttcgttcag cgcttgtaac
     8401 tgaccgttac tagtgtggat taacaccaga ccgtcgctga ccaccgggcg agaaagtgct
     8461 tcacccgcga ctttagtttg ccatgccaca gtaccatcgc tggtattcag cgcgtaaacc
     8521 tgcgcctttt cgctgccaat gtagacatgc ccaccagaca cggtcacacc gccagaaagt
     8581 aatgcaggct ctttagagaa ccagccatct ttctcggcca ggctgacaga ccagatttct
     8641 ttgccatcat ccgcattcag cgcttttacc aaaccagcgc ggtccgctgc atagacaacg
     8701 ttgtccgcca gtgccggatg aagattggaa tagaagttgc caatgccgct accaacggaa
     8761 gtgctccacg ccgtggtcgg cgtaaactgg ttttcaacgg ttggcaatgg ggacatcttt
     8821 accacatctt cttcgctgtt aaacagcgaa cagccgctta aaagggtaac ggaaagcagt
     8881 cctggcagca gtaatttacg caattgcatc gggtccctct cagatggaca aattattaat
     8941 tttcatctgc atcatttcgc tcagtgccgg agtaacatcg cttttcacgc ctgcttccca
     9001 tgcactacgc gcaccttgct tatcaccttt gctcaacaat gctttaccac gcaggtcggc
     9061 aacaatggca gcccaccctt cacctttgat ggtatcaagg gttttcagcg cggcatcagc
     9121 ctgcttgagc tgtacctgaa cgcgagcaag acgcagattt atcacggctt tgagattttc
     9181 atcactcgtg tctgccagcc cctgttgtaa ctgggcggca gctttctcta gttcattttt
     9241 gtcaacaaat tgctgcgcaa gttccaaaga agccagcgca ccataagtat ttttattttc
     9301 agcagcaaat ttttccgccg ccgggatgct atccggtttg ccttcgctca ctgcggtaac
     9361 cgcattttga taggcaagag aagcggagcg tgcagaatca acctgatggc tgttccagta
     9421 gcgccagcca atcagtgcgc caacgcccaa aatcacccca acagccagtg ctttgccatt
     9481 ttcagcaaaa aagcgtttaa ccgcttctac ctggtcgttt tcgttctcgt aaatttccac
     9541 gctgtccttc tccttcctta acccagtaac gtgcgcaaat gcgcggctac gctatcctgc
     9601 gcaactgccg tttgctcacc agagcgcaaa tccttcacta ctgctgtgcc gttagccact
     9661 tcagactcac ccagcaccac agcaacgcgg gcaccccatt tatcagcacg ggcaaactgt
     9721 ttcttaaagt tgccgccgcc gtggttggtc atcaatttca cgcccggtaa ttcatcacgc
     9781 agacgctcag ctaatgccat agccgcagat tgtgtatcag cacctgaagc caccaggtat
     9841 atatcgacaa caggatcggc tttaaattcc ggattaacgg cctgtactaa caatacaaga
     9901 cgttcgaggc ccatcgcaaa accgactgcc ggtgttgcac gaccgcccag ttgttccacc
     9961 agaccgtcat aacgaccgcc tgcacacacg gtgccctggg agccgaggct gttagtcacc
    10021 cactcgaaaa cggtacggtt atagtaatcc agaccacgca ccagacgctg gtttacggtg
    10081 taagcgatcc ccgcgctctc aagcagtttg cacagaccgg caaaatgctc acgagattcc
    10141 tcgtccagat aatcacctaa tgccggagcg tcgttgagaa gcgcctgcac ttccggattt
    10201 tttgaatcca gcacgcgcag cgggttagtg tacatgcggc gtttgcagtc ttcgtccagc
    10261 ttttctttat gctgctcaag gaatgccacc agcgcatcgc ggtaattggc gcgtgcttcc
    10321 agcgaaccaa tagagttcag ctcaagagtt acgtgttcga agatacccag cgcacgccac
    10381 cagcgggcag tgagcataat cagttcagcg tcgatatccg gaccttgcag accgaaaact
    10441 tcgcagccca actgatggaa ctgacgataa cgccctttct gcggacgctc gtgacggaac
    10501 atcggcccga tataccacag acgctgttcc tgattgtaca gaagaccatg ctcgatgccg
    10561 gcgcgtacac agcccgccgt cccttcaggg cgcagagtca ggctgtcgcc attgcgatcc
    10621 tcaaaggtgt acatctcttt ttcaaccacg tcggtgactt caccaatcgc acgtttgaat
    10681 agcggggtct gctctacaat cggcaagcgg atttcactgt aaccgtagct gccgagcacg
    10741 tttttcagtg tgccttcaat gcgctgccag atggccgttt cgccaggcag gtaatcgttc
    10801 atgccgcgaa tggcttgaat gttttttgcc acgtttattc tctttctgaa tataaaaatg
    10861 aaccctcaac gcttccctca atgtttcggg agccatgcgg gttcaatcat acacgggaag
    10921 cgaggcgctt cccatcacgt tattattttt caacctgctg aacgtcaatt cgacgcgctt
    10981 cgtccagctg actggctttc gcacgaatgc gtgcttccag ctggtcgatc atatcgttgt
    11041 tgtccagacg gtctttgcgc acgccatctt catagaggcc gcttttcttg ttgccgccgg
    11101 tgacgccgag tgtagaaacc agcgcctcac ctgggccatt caccacgcag ccgataatcg
    11161 aaacgtccat cggagtgatg atatcttcca ggcgttgctc cagcgcgtta accgtaccga
    11221 taacatcaaa ttcctgacgc gaacaggtcg ggcaggcgat gaagttaatc cctcgcgaac
    11281 ggatacgcag cgatttcaaa atatcgaaac cgactttgat ctcttcgacc ggatcggccg
    11341 ccagcgatac gcgcagcgtg tcgccgatgc cttccgacag cagcagacct aaaccaatgg
    11401 cggattttac tgccccgctg cgtgcaccac cggcttcggt gatccccaga tgcagcggct
    11461 gatcgatctg ttttgccagc aaacgataag actcaacagc gaggaagacg tcagacgctt
    11521 tcacgctgac tttgaactga tcgaagttca ggcgatcgag atgatcaaca tgacgcatgg
    11581 cggattccag caacgcctgc ggcgtcggtt cgccatactt ttcttgcaga tctttttcca
    11641 gcgatccggc gttaacgcca atacggatcg gaatgttttt atcgcgcgca cagtcaacca
    11701 ccatgcgaat acgctcttca ttaccgatat tgccagggtt aatacgcaga caatcgacgc
    11761 cgtattccgc tactttcagc gcaatgcgat agtcgaagtg gatgtcagcc accagcggca
    11821 cgttaacccg ctgtttgatg agtttgaacg cttctgccgc gtccatcgtc ggtacggaga
    11881 cacggacgat atcagcgcca acgcgttcca gcgccttgat ttgattgacc gttgcttcga
    11941 catctgtcgt acgcgtattg gtcatggact gtacggcgat gggagcacca tcgccaatcg
    12001 gcacattccc aacgtaaata cgtgttgatt ttctacgttg aattggagcc tggttatgca
    12061 tgaaaaatct cccgcgttac ccgtctgtta ctgcgccggt gattgttcgg cattgagggt
    12121 cagacgcgca acctggttag ttctgataaa acgactcaga tcgacaggtt tcccttgata
    12181 ctggatctgt actgcggctg gcgcaccaat tttcagttta tacggtgcct ggccggttaa
    12241 attcaaatta ccgtctttac gctgcatccc gctaaacaat tttttaccgg tagcatcagt
    12301 gacctccagc cagcaatcgg cagtaaagtt catcaccagc gcattcggat cagccgccgg
    12361 cgtggtcacg ccagcctgat cggttggcaa cggcgcagca ccatctggcg ttgttgctgc
    12421 cgttggtacc ggggtcgcgg cggtatcaac atttgcctgc gaaggcgaaa caaccgcatt
    12481 ctgttgcgga tcaacagctg gtgctggcgc agttacggca ggtgtttgcg tgttggttgc
    12541 ggtagtatcc acagacgctg gcggcgtgga cgttgtagcc gggtctgtag ttgtcgacgt
    12601 atttaacggg acactctgcc cctgctcgct attactgctc agttctgccg aagattgatc
    12661 ggccatagtg gtgatctctt cctgctgagc tttgtggtct tgccaccacc aggcaccgct
    12721 caggccgata accacaaaca acaccagcca ggtgaaggtc atcagccagc catcacgttt
    12781 tttgcggcgt ttaccgaggg aaaaactctg cattggcgca acttttgcag cccgtagtgg
    12841 agcctgcttt tccagccctg gcagcagttc ttcttctgga atatgtacca gacgcgcata
    12901 agagcggata tatccgcgca ggaatgttga agcaagatcg gcgggtgcct tatcttcttc
    12961 aatgtcgcgt accgtggaaa ccttcaggca aagtcgctcg gcaacggcct gctgactaag
    13021 tcctagttgt tcgcgagcat tacgcaggcg agcgccggta gtaagtgctt cattttggtc
    13081 gtgcgtggct tcagtattca ttcgctgcag gtacgtttaa atgagaattg agatgccggt
    13141 gaaccatcat gctcacccac accgcgaaac atccgttaag ttaaccgtta tcatacagta
    13201 taagaccgtc tgtgtgctcg tgacaaagcc cacattcact aaggctaact tactgttgca
    13261 tcgttacata ctgccttaaa gtcagcaaaa acgcaccgtt aatattgacc agacaattgc
    13321 aacttaatac ttcattcata aaacattacg ccacggtaca taaagtaacc gtggcgtaat
    13381 ggctatcaga ccgctttaat gtcgatggct tcaccctgca tccgtttacg cagggtacgt
    13441 ttcgtacggt cgataacatc gcccgccaac tgaccacagg cagcatcaat atcatcacca
    13501 cgagttttac gcacaatagt ggtgaaaccg tagctcatca gcacttttga gaaacggtcg
    13561 atacggctgt tcgagctgcg tccatacggc gcacccggga acgggttcca cgggatcagg
    13621 ttgatcttac acggcgtatc tttcagcagt tccgccagtt ggtgcgcgtg ttcagtgccg
    13681 tcgttaacgt ggtcaagcat cacgtattca atagtgactc ggccctgatt ggcgttggat
    13741 ttctccagat aacggcgcac cgcagcaagg aacgtttcga tattgtactt tttgttgatc
    13801 ggcacaattt cgtcacgaat ttcgtcgttc ggcgcgtgca gggaaattgc cagtgcaacg
    13861 tcgatcatat cgcccagttt atccagcgcc ggaactacac cggaagtaga aagcgtgacg
    13921 cgacgtttag acaggccaaa accgaaatca tcaagcatga tttccatcgc cggaacgacg
    13981 ttgttcaggt tgagcagcgg ctcgcccatg cccatcatca ctacgttagt gattggccgc
    14041 tgaccggtga cttttgctgc gccgacgatt ttcgccgccc gccacacctg gccgataatt
    14101 tccgacaccc gcaggttgcg gttaaagccc tgctgggcgg tggaacagaa tttacactcc
    14161 agcgcacacc ccacctgcga agagacgcag agcgtggcac ggtcgtcttc cgggatatac
    14221 accgtttcga cgcgctgatc gccaacggcg atcgcccatt taatggtgcc gtcggatgaa
    14281 cgctgttctt caaccacttc cggtgcgcgg atctccgcca cctctttcag tttgccgcgc
    14341 aacactttgt tgatgtcggt catctcatca aagttgtcgc agcaatagtg atacatccac
    14401 ttcatcacct gatcggcgcg gaagggtttt tcacctaaat ctttaaaaaa ctcccgcatc
    14461 tgctgacggt tgagatccag caggttgatt tttccatctt tcgtggtgac gttttcaggt
    14521 gtgactaatt gttcagacat atgctattcc ggcctcgtta ttacacgtta tggcccctgg
    14581 agggttgaaa aaagaaacgc cccggtgagc ggctgctcgt ccgggggcgc tgcattgtac
    14641 aaattctggc gcacggatgc cacgtttgca cgcggcattt acgaaattat taacgggtgc
    14701 gcgggcacac ttcgccttcg ccgaagaaat aagcgatttc gcgagcggca gattcgacgg
    14761 aatcagaacc gtgggtaccg ttttcggtca ggctgtcagc gtaatcagcg cgcagagtac
    14821 cggccagtgc gtttgccgga ttggtcgcgc ccagcagatc gcggtgacgc tgaacggcgt
    14881 tttcaccttc cagcacggaa accacgatcg ggccagaggt catgaattca accagaccat
    14941 caaagaacgg ttttccatcg tgttcagcat aaaagccacg agcctgttca acggtcaggt
    15001 gcagcatttt ggtgccgaca attttgaacc ctgcagcttc aaagcgcgca aagatattac
    15061 caatgacgtt ttttgctacc gcgttcggtt tgatgatgga aaaagtacgt tcaatagcca
    15121 tttttacctc tgtaaattgt tctgttgttg tctgtaccag cgtacgagat ggcgcggatt
    15181 ataatgagca acagggccgt tgactattga tgaaggtaac atttttttaa aataaaatga
    15241 gttttagcaa caatcaggga caaaacagac tattgcatga caaatttcac tgtcgcgatt
    15301 tgccccacgt catccatcac cagcaattga taatcacctt tatccgtcaa atgcagggtc
    15361 acgttgcgcc cgcgttcagt taacggttcg ccattcagaa accaccagcg ttcacctgcc
    15421 cctccacttg attgcaacgg caaagtcgct tccgctgcgc ccggtaaacg tttaataatc
    15481 gcgccatcgc gaacgcctgt cagctgcaga gggagctggg catcgtgacc gtatggcggg
    15541 caaattgtcg aggccggtgg taagcgcaca gcgcgacgtt ctgatgcggg cagccagggt
    15601 tccagcggta gcggccagac atttaccatt tcttgcttcg cctgcgggca gtcagcggcg
    15661 acgcgctgcc ctttgtcatc cagccagatg gggaagcgaa tgccattaat cccttcctgc
    15721 tctgacagta acagcgttgg cggctggctg ccatccagca gccaggttgc caggcggcgg
    15781 cgacagttac cgtcaccttc cggcaaagac tgtccgcccg gccagcagat aacgccacga
    15841 gtgactgagt tcgggcgcgg gtcttccggc agattcgcac tgcgcgacag taagatatta
    15901 ttgacctgat tcaacaatgg tacggcactg gcaaagccaa actgaccaac aacgggcgtg
    15961 ccgtcaggtc tgccagtcca gatcccaatg acatagcgag cgttaacccc aatcgcccag
    16021 gcgtcacgat agccatagct ggtgcccgtt ttccatgcca gtggggcgac gcgcggcaag
    16081 gcactatccg gcaagggttg cgcttcatca gccataatcc ggcgaatgat ccacgccgcc
    16141 cccgacgaca ttaaaggccg ttcaagcagc ggatcgtcag gctgtaagcg caatttgcct
    16201 gccttgccgt ggcgagcaaa cgcggtatac gctgccgcca tatcttccag ttttgcacca
    16261 gcgccgccga gaatgagtga aagattcggc gcagcgccgt tgggcaaata taacggcaat
    16321 ccaacattgc gtaactttgc cgcaaaccgt ttcggtccat aggcttccag cacctgcaca
    16381 gcaggtaagt tcagcgagcg caccagcgcc tcgctcatgc tgatcgggcc atgaaaaccg
    16441 ctatcaaagt tacctggtcg ataatcaccg gtgcgccggg ggacgtcttg cagcagtgat
    16501 gccgggtgga tcaagccttc atccagcgcc agaccataaa caaacggttt gagcaccgat
    16561 cctggcgatc ggatcgcatt gaccatatca acatgaccaa agcgtgaatc atcgttgaga
    16621 tcaaccgatc ccacccagcc gcgaacacgc atatcggtat gatcaaccac gatcatcgcc
    16681 agtgagctgc gcggtggcaa ccgccctttc cagttttgcg ccagttcttc cagacgtcgt
    16741 tgaagaccgg catccagcgt agtaacgatt ttgtcgcttt tgcttttacc gagcatcatg
    16801 cgcgaaaaca gcggtgccag ttgcggcatt tgtcggggtg ccagccagat gggttcttcc
    16861 cttgactctt ttacctgctc acgggaccac acaccttgcg cagccatccg ttcgagcact
    16921 ttattacgcg cggcttcggc acgctccggc caacgatccg ggcgaagacg gctgggcgct
    16981 tgcggcaaaa ccgccagcat tgccgcctcg gaatagctta aattcgcagg cgactttccg
    17041 agataagccc aacttgccgc accgatcccc tgcaacgtac cgccaaacgg agcgcggtta
    17101 agatacaagg tcagaatttc acgcttagac agatgccatt ccagttgcaa cgcgcgccag
    17161 agctggcgaa ttttgccgcc aaatgttttg gggtgaggat caagcagacg agcaacctgc
    17221 atagtgagcg tgctgccacc ggaaataacc cgtcccgaag tgagatcttg ccatgctgcg
    17281 cgcgccaccg agaatggatt cacccccgga tgcttccaga accagcgatc ttcataattg
    17341 atcagcgctt caaggtaacg tggagaaaca tcttcgattg ttaccggata acgccagatg
    17401 ccgtcagcat cggcgaagcg ccagagcggc gtaccatcct gcgccacgac cactcgtgcg
    17461 ggattgactt catgcagcgg tagaggccag agtttatctg ctccccacgc tgcaagaaaa
    17521 aggagaaatg gcgcagctgc cagcgttatc cagcagccgc gtttggttaa caagcgaggc
    17581 atttacggtc ggacaatcag cagatcttca gccgcgccgg tcgcccgcca ttggggaaca
    17641 tacattgatt ccaccatcgg ttgcggtacc tgatacgttc ccggcgtcac cgcccgcgcc
    17701 agatacacca gcgttaccgg ttggtattca tcaacggcaa ccgccgccac aaagcgatcg
    17761 tcacggaact caatgtgctt aatactcgcc tgctgcatct ggttcagtaa gttctgcact
    17821 tcgccaccac tttgctccag gctggcgcta ccgttcgcca gattctggtt ttccagttcc
    17881 agacccacag gcagcagatc cacgactaac gcatccggca cgctgttact ggcttttacc
    17941 tgcaaccaca ccagcaccag atcgccgcta cgtaacgagt ccagcgattt gctcttacca
    18001 tcagtaccaa gaatatgacg ctcgatttgc agcacattgt tcgccggtaa aggtgcggat
    18061 tgcggataac cgctggcatc catacgcaac cataacggct gatcgccact gttgctcacc
    18121 tgcaaggtga caagttgatc gctattcaga ttgctgtttt gcgctttctc gcctgtcagc
    18181 ggctcagctg agaaagaggt ttgcgcctgc catttacccg gtaaatcctg aatcgtacgg
    18241 gcagccagga acaacgcgtt actttcctgc gtcgatagcc agcgttcacc aaacgcctgc
    18301 tgcgaaagtg tgttcagcaa agtgtactgc tcatcgggta gcagtttatt ttcttccagc
    18361 aaggagagca ttaacgcgtt gtcgcgcagt ggactaccgt aatcacccag ccatatccgc
    18421 tcatcactat ttctcggcgt tttcagcgcc agcgcaatcg cttcttcacc acgcgtcgca
    18481 tcacccatgg ttttcagcgc aatgccaagt tgcagcagcg gtaaaccaga agcggcatct
    18541 gcacgatgct cccagatttc acgcagcgca cccagtggtg ctttttgctg acgggccagc
    18601 accagcgcag cgtaagactg aacggcgaat ttactggctt tgagattatc cgcgtacggg
    18661 atcgacatca tgcccggatc ttgtaaatag cgcagcagac gctcattacc ccggttaatg
    18721 gcgtctgtcg gcacgctgta accctgttcg cctgcgcgga ccaggaaatc catcacgtaa
    18781 gccgtcagcc agtactcttc gtcaccgttt ttatcccaca gcgcaaagcc gccgttatca
    18841 cgttgcattt gcagcaaacg ggaaatgccg atatcgaccg atgcacggcg tttctcatca
    18901 ctgtcgcctt tgatgcccaa cgcctgcagt tgggctgcgt tggtataaag tgacgggaac
    18961 aggccgctgg cggtttgctc aagacagccg tacggatacg cttttaactc tttgatataa
    19021 cgtgcaatgt tcagcggtgg tttgccgctc aacaacaatt gcccttccag cgtaacaggc
    19081 gagaagtttt gcaatccatc cgccggaatc gcccatgtct caccaggctg taacgccgta
    19141 ccgtaattaa ccgtttgtgc cgggaacgcc ggacggacgc cgattttcca ctgcttatgc
    19201 tgatcggcaa cggtttcacc cggtaacgct aacccgctaa tggtggcctg aatttctcca
    19261 tcgccataac ccggcaatgc tcgcaccggg ataaacagcg tagtacgcac acctggcgct
    19321 aattcaacgg ctgcgggtga atcgctgacc agttcaagca aaccactggc ggtcagggca
    19381 acgttcagtt tttgcggttt atcggtaaga ttagtgatat ccagcgtcag acgcgaggta
    19441 tcgccactcg ccataaagcg cggcatgttc agttcagcaa tcaccggtgc ggcaactatc
    19501 actttgcttt cgttgctacc gaaatcatct gccgtccagg cttgcgccat gacgcgcaat
    19561 tcaccgttaa aatcgccaat cggcagtgta accgagcctt cgccctgttc gttgagcgtt
    19621 accgacagcg cctgctgcgc gacaatattg acgtgattga ccggcggttt accaccacgt
    19681 ttcagctcat caccatcgcc accgaaacgc agagctgcca gacgcccctg accttcaata
    19741 acctgaccgt aaatatcgta aatgtctgcg ccatagcgtt tctgaccaaa gaacgcctgc
    19801 cacggatctg gcgtaacgta gtcagtaata ttcagcacac cactatcaac ggcagacacc
    19861 agcacattca cctgtttagg cttctcgcca tttttagtgc tggctttaat tttcacggtt
    19921 aatggttgat tgggacgcat ttttgctggt gtttccagcg ccagatcgag gcgacggttt
    19981 tcatcaccaa gcggcagatg caacacacca accgcgcgtt ttggcgtcgc ggagcgagat
    20041 ttatcgccag gacgcaccac cagcgtactc aaataaagat catgacgatt ccaggtttta
    20101 tcgaccggaa tcgtcagatc cagcccttga gccggaacat caatctcttg ccaccacagc
    20161 ggcccttcac tggactcgac catcgcataa cctttacccg ccgttggcgc ggcaatatgc
    20221 aacttaatgg tatcgccggg gcgataactg gctttatcta gtttcagcgt gacacggtcg
    20281 ggtcgcactg cgccgctccc gtcgctgttg tcctgccagc tatagccagc ccaaaaacgc
    20341 acgctactga ccgcttcatt cggcgctttg acttccagac gataagcgcc ccactctacc
    20401 gggaaactga ccttaccggt ttcatccgcc tgaagatcca gagtttgttc attttcgatc
    20461 agatcttttt gatcaaactg tgactgccag ccttcatctt ctgaccagtt ccagtagtaa
    20521 tcgcggcgtt cgcgaatcag gcgcacctgc aagcccgaca cggctttttt cacgccttgc
    20581 gcatcgctat aaacgatgtc aaaagcggcg ttactgcctt catcaacaat gggctgtttc
    20641 accgtgctgt cagtgcgata atcgtaaacc gatttcaagg cgaactgcgg acggagcccc
    20701 ggcaatgcat cggcaggcca gatagcctgc tcagcgcggc gcgtcaccgg gcgaccgccc
    20761 gattccagca aactgccctg gaaaataacc tgcaatgggg aatgcgtttc cttccactgg
    20821 ctttctgtag aaacttcgcc gcgcccttta tcatccagcg tcaactgaac ttcatccagc
    20881 gtgcgggaaa ggttttcggc agctatatcg ccgaattcaa aaccaggtaa ggctgacaca
    20941 gcctcacgca gtgggcgcag gaaaagttgc ccttgcaaag tattaccatt agcaggcgca
    21001 ccgtacaggt agtatcccac cacggagaat ttcacttcat cattcggcgt aagcggggtt
    21061 ttctcaccgg tcagattcag cgccatacgc tctggcataa aatcttcgac gtggaaatcc
    21121 cacatccggt actggttatc gcccgtgttg gcgcgaatat gccacatacc ggttgccgca
    21181 ttgctatcga gtggccaggt aaagtggtag aggccattct ccggctgact aacgacgttc
    21241 ctgagtacct gcccgtcagg tttaatcacg tctaacttga tgggttgatc gggcaatgct
    21301 ttaccgtctg catcgcgcag taaaccattg aggattaccg tttcacctgg gcgataaaga
    21361 tcgcgtgggc caaacatgaa aaactgtttg ctatagcctg gcgcgccagc aatgttaaat
    21421 tctgctaagt ccagcgccgg aagttttaaa tcgagtagcg ttgtctgacc gtctttacgt
    21481 gccagcagta atgccgcatt tttatcattt tccagctgca catgcccctg agcgtcactg
    21541 gttgcctgag tcagagtctg tcctttctca tttaataaag agacttcaat tccttgctgg
    21601 gccgcgccgt tttccagact ttgggtaaag atatccagac gattgtgata acggtgagct
    21661 gaaacgccga tatcacttaa cgtaaacagc gtcgcgggat tactgtaatc gtaacgtcca
    21721 gcctgattca tcacagccag atacacgccc gcctgctgaa gcggtttgat atcgcccagc
    21781 ggcagcaata atttttcacg ggtgttacgc gcaggattga gatcaaaccg tccggtgtag
    21841 accagatccg ccatctgcag cagtttgtct gactgccagt tcgccagcga attgcggtat
    21901 tcccattggc taatgaatgc tggcagagat tctggcttaa cgcggaagaa gttaacatcg
    21961 acattattaa cgttgagcgc cattaccggc agcccttcaa cgactttgcc aggcagcagc
    22021 gaaccacggc tggcaaaacc gacgctgggt tggatgtcgc gggtagttat agttttttcg
    22081 taatctttac tgaaggttgc gttgttgagt gctttgacct ccttgccaat agtaacgatc
    22141 aaatcacgtt tcggttcgag gtggcgtaaa cgtagctctt taagattatc tgacagctcc
    22201 caggcaccat ccactttgcc gcttttttta tcgacgacat gaataacgcg tgagaaatcc
    22261 tgatccgggt cgagagggat ggagaacgtc agcaccagcg tggctgcacc atcaagttgg
    22321 acttcagaga gatccagcaa tgtcagcacc ttaccggcac tctgttgggc cagtttttgt
    22381 ctctccggca cggagagttt tgctgaactc gcgttttctg aagaggccgc tttagtaact
    22441 tcagaaggcg catctttttt caccgctgtt ggcgcgttat cgttgttgtc gcaccctgcc
    22501 agcgccagca ttagcatgca ggcggctacg cgtaactttt tcatttttca tccctgccac
    22561 aatggcccgt tagcaacgtc gaataattat tatgcgtgag aatttacgtt atgtaattca
    22621 gtatcacgcc catacaatcc gaattttagt gagaattgtg gctcagacag ccaaatcagc
    22681 ctctttcgct tgtcgctttt cactaaacat ccgacaattt agcctaaccc cggcaaaaat
    22741 ggagatgcct atgtccacga catggtttgt aggagccgac tggctcgccg aacatattga
    22801 tgacccggaa attcagatta tcgatgcccg catggcgtcg cctggacagg aagatcgtaa
    22861 cgttgctcag gagtatctga atggacatat tcccggcgca gtgttttttg atatcgaagc
    22921 gctttctgat cacacttccc cgcttccgca catgctgccg cgcccggaaa cgttcgccgt
    22981 ggcgatgcgt gaattaggcg ttaaccagga taagcacctg attgtctatg acgaaggtaa
    23041 tcttttctca gccccacgag catggtggat gctgcgcacc tttggtgtag agaaagtgtc
    23101 gattctgggg ggtggacttg caggctggca gcgcgatgat ctgctgttag aagaaggtgc
    23161 agtagagctg ccggaaggag agtttaacgc cgcgtttaat cctgaagccg tggtgaaagt
    23221 aaccgatgta ttattggcaa gccatgaaaa tacggcgcaa attattgatg cccgcccggc
    23281 tgcacgtttt aacgcagaag ttgatgaacc tcgcccaggt ttacgtcgcg gacatattcc
    23341 cggagcactg aatgttccgt ggacggaact ggtgcgcgaa ggcgaactaa aaacgaccga
    23401 tgaactggat gcgatatttt ttggtcgcgg cgtcagctac gacaaaccaa ttatcgtcag
    23461 ctgcggctct ggtgtaacgg cagccgtggt tttgttagca ctcgcgacgc tggatgtgcc
    23521 aaacgtgaaa ctgtacgacg gcgcatggag tgaatggggc gcgcgggcag atttaccggt
    23581 tgagccagtg aaataagtat tttacaggca ataaaaaacc gccgaatttg gcggtttttt
    23641 attgctggtc tggttcgcgg cctttccagc aggttgtatt accgtagtaa tgcaagcgcg
    23701 tctcagcgga gacaatactc gccagtaact ctctttttgt caagcaaaag agagttatta
    23761 ttgttctgtt agtgtattat ccactgcggc cctttccgcc gtctcgcaag cgggcgctgg
    23821 cttaaggaaa ggatgttccg cgaccgttag agcaagcgcg tctcagcgct tgctatcgcg
    23881 gcaatatcgc cagtggtgct gtcgtgatgc ggtcttcgca tggaccgcac aatgaagata
    23941 cggtgcttct gtatcgtact tattgtttct ggtgcgctgt tttcccaagt aaataataac
    24001 cggagtctct ccggcgacaa tttactggtg gttaacaacc ttcagagcag caagtaagcc
    24061 cgaatgccgc cctttagggc ggcatatttt agattatccg gttttgttta aagtcacgca
    24121 aaaaaccacc ccagcgacgt tcatagaatg gcacaatatg ttcggtaata aagtggctaa
    24181 ttcctttttc cccttttttc acctgacaaa tatcgattgg ttcatcgcca ggtaatgtat
    24241 cggtcgctac acttcccgtc gcctgaataa tttcttcgat atcaccatcg gcttcaatgc
    24301 caataagtaa attaggctgt gcctcttcgt tctctttaat tgaacaaata aaagcacgct
    24361 tcaccggctt aatggtttta aataaggtgg tgagtgaatc aatcatttgt gctggcggct
    24421 ctgcgacttc cgataatatc agcgattcac cgccttccag gatttcctgg ctgctcagcg
    24481 gatttccctc ttcaccaatc aacaaactga tttcacgcgg cataaattct ttaccggtcg
    24541 gcagtttggc attaaggaag agcgtttcgc caagggtcat ctcaaacaat gtgcgaacgg
    24601 gcattacgac aaatgcctgt tcgtcttcaa ccgcctgttg aagcgcttct aacgaggtga
    24661 aaaaaggaat gacgctggtg ccgtcttctt tttcccagtg ctgtaaatca agcgcactat
    24721 cttcaaccac agcctcgccc tgcgccgccg taccaggcac ccagacggtg gattccagta
    24781 gagtacggaa aaaggccggg cggtgcgccg gttcggttgc tgctttttcc agcaggtctt
    24841 caagttcgtt ttttgtttcg gacataagaa ccacaattca ttcaacgttc ggggcgcaaa
    24901 tgcctgatgc gctacgctta tcaggcctac aagggattcg caatttgttg aatttgcaga
    24961 atttgtaggc cggataaggc gtttacgccg catccggcat attagtttac gccgttaaca
    25021 gattagctat cgtgcgcaca ccaagtcccg tagcgcccgc agaccactgt tcaaccggcg
    25081 ctttacggta agtcgccgag cagtcgatat gcagccagcc ttgctgatag ttctcaacaa
    25141 agtgcgacag gaagcccgcc gccgtgctcg cgcctgccgg atacgccgcg cttccggtat
    25201 tgttcagttc ggcaaagtta gacggcagct ggctgcggtg gaattccgcc agcggcagac
    25261 gccagaatgg ttcgttctct tgtgaggcac tcgccagcaa acgaccggca agcgcatcgt
    25321 caaaactgaa cagcgcgtga taatcattac ccagcgcagt tttcgccgcc ccggtgaggg
    25381 tcgccgcatc aatgatcatt tccggtttct gcgcactggc atcaatcaga ccatcggcaa
    25441 gcaccagacg cccttcggca tcagtgttca tcacttcaac ttttttaccg ttgcgatagg
    25501 tgatgatatc gcccagcttg aacgcattgc cgctaatcag gttatccgca cagcagagga
    25561 acagcttcac gcgcttgttc agtccgcgcg taatggcaaa tgccagcgcc ccggtaaccg
    25621 ttgccgcgcc gcccatgtcc gacttcatcg agtccataaa cgcagtctgt ttgatgctgt
    25681 agccgccgga gtcaaaagtg atacctttac ctaccaggca cgcgtacact ggcgcttctt
    25741 tatcgccagt tgggttgtaa tccagcgcca gcaataccgg agaacgttct gaaccgcgtc
    25801 cgacggtgtg cagccccata taaccttgct cacgcagatc ttcgcctttg gtgatccgat
    25861 aagtcacacg atcgcccgcg acgttgctga tcagatcaac agcacgctgt gccagttgcg
    25921 atggtcccaa ttcttctgcc ggtgcgttga tggtgtcacg cacccagtcg atgatcatca
    25981 ggcggttatc cagttcctgg cgctgggcat cgtccagatc cggccactcc actttacgcg
    26041 tgcctttcgg ggctttgtaa ccttgccaga atgcccagca gcgatccgca tcccagcctt
    26101 caccgcttaa ctgaacatgc ttgatgccca gaccgtcaat cttgcgcgcc gcacgctgga
    26161 tcagccccag atcgtctgcc ccgttcaaat gcagggtaat gccgtcatta ttaatgctgt
    26221 aagttgcttt ttctccccag cgcgcgtcgg caggttgggt agagagggta atcttcatcg
    26281 cttctgtcat tttagttatc cttctttgta aaagggccgc cagttggcag ccccaggatt
    26341 tttccgctaa atgattcgcg ttgcagaaag gcggcaagtg agtgaagccc caggagcata
    26401 gataactatg tgactggggt gaacgagcgc agccaacgca tctgcggcgt gaagcatgac
    26461 gcggaaatta ttcggcctcg tccagccaga ctaacaaaat cgcttcgagg attttctcgt
    26521 tggatgcctg cgggtcgtcg tcgaaatctt ccagatcgca aatccactga tgcatatcgg
    26581 tgaatcgaac cgttttcgga tcaagatcag gatacgcatc gtacagtgct tcgccaattt
    26641 cgcggctatc ggtccactta agtcccatac taacctctgt taatgctcac gcgcatggtt
    26701 gatagtgtaa cgcgggattt cgactactaa atcttcgtcg gtaacgcgtg cctggcagct
    26761 taaacggctt tcgggctcca gtccccaggc tttgtccagc atgtcgtctt cctgctctga
    26821 gctttctggc agcgagtcaa aaccttcacg aacgatgcag tggcaggtgg tgcaagcaca
    26881 ggatttttca caggcgtgtt caatctcgat accgttacgc agagctgcgt cgagaatggt
    26941 ttcaccgcta ttagcttcca gaacagcgcc atcaggacag agatcctgat gaggcaaaat
    27001 aacaatcttt ggcatattaa acctcgtcca cggaatggcc tttcagcgca cgacgaaccg
    27061 actggtccat gcggcgagcg gcgaaatcct gggtttgttt gtctacgttt ttaatcgctt
    27121 gttcgatggc gtcaacgtca tcgccctgcg ccacttcact caggtgagcg gcagcatcgt
    27181 caatgacctg acgttctgcg gcgcttaaca gcgcggcatc agcagccagc gcgccgtgca
    27241 gactttccag cacacgcgcc gcttctactt tttgttctgc cagcattcgg gcttttacgt
    27301 cctgctcggc atagctcatt gagtctttga tcatcgaagc gatttcgcta tcggtcagac
    27361 cgtaagacgg tttgacctga atagacgcct caacgccggt ggatttctcc atcgccgtca
    27421 cgctcaaaag accgtcggca tcgacctgga acgtcacgcg aatatgcgca ccaccagccg
    27481 gtagcgccgg aataccacgc agcgcaaaac gcgccagtga gcggcagtcc tgcaccagtt
    27541 cgcgctcacc ctgcattaca tggatagaca tcgccgtctg accatcttta aaggtggtga
    27601 aatcctgagc gcgggccacc ggaatagtgg tattacgcgg aatcactttc tccaccaggc
    27661 cgcccatcgt ttcgaggccc agcgacagtg ggatcacatc aagcagcagc atttcgctgt
    27721 ctggcttgtt acccaccaga atatccgcct gaatcgccgc gccaatagcg acgactttat
    27781 ccgggtcgat ggaagtcagc ggtggacgac cgaaaaattc gcctacccgt tcacgcacca
    27841 gcggcacgcg agtagaaccg cccaccatca ccacttccag cacttcgtca gcttctacac
    27901 ccgcgtcttt aagcgcgcga cgacaagcca gtaaggttcg ttttaccagt ggcgcgatca
    27961 gttcattgaa ttgttcacgg ctgatttcgc cctgccagcc cgcaacgtta acggtcacgg
    28021 agtccgcatc gctcagcgcg attttggctg caatggcggc atccagcagt tcacgctgaa
    28081 cgcggttatc gctacgatca ggaatgcccg cctgctcgcg aatgtaatcc gccagcagat
    28141 ggtcgaaatc atcgccgccg agcgcggaat caccgccggt tgccagcact tcaaacacgc
    28201 cgcgacttaa gcgcagaatg gaaatatcaa acgtcccgcc accgaggtca taaacagcga
    28261 tcacgccttc ctgaccggaa tccagcccgt aggcgatagc cgcagcggtc ggttcgttaa
    28321 gtaagcgcag aacatgcaac cccgccagac gcgccgcgtc tttggtgccc tgacgctggg
    28381 catcgtcaaa gtacgccgga acggtgataa ctacaccatc cagctcgcct gccagggctt
    28441 cagttgcccg cgccgccagt gctttgagga tgtccgcaga aacgcgcacc gggttcagca
    28501 gccccgccgc cgtttcaatc atcggcaggc cgttttcgct ggcctggaat tgataaggca
    28561 gatgcggata gcgttgctgg atatcagcca gcgagcgccc catcaggcgt ttaacagaac
    28621 taatggtgtt ggcggtgtca agcgctgcgt tagtacgcgc gtcataaccc accgaatgcc
    28681 cttgctgttg atagtgaaca acagatggca gcaggtgacg gccttcatgg tcggctaacg
    28741 tttcggcctg accgctgcgc actgtcgcca ccagcgagtt ggttgtgccc aggtcaatac
    28801 cagccgccag acgacgctga tgcggcgcag cactcaaacc aggttcacta atttgtaata
    28861 aggccatgtt tagcttccag aaattaaaaa tcgagcagtt tttcttcgag ttgttcggca
    28921 ctgctccgca gtttatcgag aaaacgcagc ttacgcacgg tatccgccgc cgcgtcccac
    28981 gcctcgttgt ctaactgttc aaccatcaac tgatggcggg tatcaaacat ctttttcaca
    29041 cgtttgataa agctttccag ccgcgcttca tctttcgcct gttcgatctc gtccagctct
    29101 tcgcgcagct ccaactgttc catcaggaac gcggtgtcgc gcacagtatg ctgctcgctg
    29161 gcgagatcaa aaccgtgtaa agaaagcaaa tattccgcgc gcattaacgg atgacgcagc
    29221 gtttgccagg cctggttaat ggttgcagat tgctgtacgg cggcgagttg ttccgcctgg
    29281 cttccgctgg cgaatttatc aggatgatac tgacgttgta gatcctgaaa acgcaggctc
    29341 agcgcctggg tatcgagttg ataacgggca ggcaagccaa agaaggtgaa gtaatccata
    29401 acattctcag ggctgcgatc cgccgcgata acggcgaatc aggtaaaaca aaccccacgc
    29461 gcaggcgacc acggtggggt tatcggtatg cgcatcaaac gtggaagctt tcgccgcaac
    29521 cacactcatc tttgacgttc gggttggtga atttaaaccc ttcgttcagg ccttctttta
    29581 cgaagtccag ctgcgtaccg tccagaaatt gcaggctttt gccatcgacc acgactttca
    29641 cgcctttgtc ttcaaacacg atgtcttccg gcgtcggttc gtcaacaaat tccagtacat
    29701 aagccatacc tgaacatccg gaggttctca cgcccagacg caggccaaac cctttgccgc
    29761 ggttagccag aaaggtattt actcgcgctg ctgcactgtc gctcagtgta atcgacataa
    29821 ccaaacctca actcttattt tgcttcacgt ttgcttttat agtccgcaat ggcggctttg
    29881 atcgcgtctt ctgccagaat agaacagtga attttcaccg gcggcagttc aagttcttca
    29941 gcaatatcgg tatttttgat cgcctgcgct tcgtcgagag acttcccttt cacccattcg
    30001 gtgaccaggg agctggaagc gatagcagaa ccgcagccgt aagttttaaa acgcgcgtct
    30061 tcaatgatac cttcatcgtt gactttaatc tgcaacttca tcacgtcgcc acaggccggt
    30121 gcccccacca tgccgctgcc gacgttctcg tcgttgttgt caaaggaacc cacgttacgc
    30181 ggattctcgt aatggtcgat aactttttcg ctgtaagcca ttataaattc tcctgattcc
    30241 gataccgatt aatgatgagc ccattcgatg ctgttcagat ccacgccctg cttgtacatt
    30301 tcccacagcg gagaaaggtc acgcagacga ccgatggatt tacgaactaa ctcgatggtg
    30361 tagtcgatct cttcttcagt agtaaaacga cctaaagaga aacggataga gctatgtgcc
    30421 agctcgtcgt tcagccccag cgcgcgcagc acgtaggacg gttcgaggct tgctgacgta
    30481 caggcggaac ctgaagaaac tgcgaggtct ttcagcgcca taatcagcga ctcaccttca
    30541 acgtagttga agctgacgtt gagaatgttc ggcgcaccgt gttccaggtc accgttcagg
    30601 taaacttctt cgatatcttt gatgccgttc cacagacggt tacgcaggcc gcgcagacgt
    30661 tccatctcgg tcgccatctc ttcttttgcg atgcgatagg cctcgcccat tccgacgatc
    30721 tggtgaacag gcagagtgcc ggaacgcata ccgcgctcgt gaccgccgcc gtgcatttgc
    30781 gcttcgatgc gtacgcgcgg tttacgacgt acatacagcg caccgatacc tttcgggcca
    30841 tagattttgt gaccggagaa agacatcagg tcaactttca actggctcag gtcgataggc
    30901 agtttgccca cgctctgggt tgcatcaacg tgatagataa tgccacgagc acggcacatt
    30961 tcgccgatag ccgcgatatc ctgcaccacg ccgatttcgt tatttacgtg catgatggaa
    31021 acaaggatgg tgtcatcacg catcgctgct tcaagttctt tcagatcgat aatgccgtta
    31081 cgctgcggtg ccaggtaggt gacttcaaaa ccttcgcgct ccagctgacg gcaggtatcc
    31141 agtaccgctt tgtgttcggt tttgctggtg atgatgtgct tgcctttttt ctgataaaag
    31201 ttggctgcac ctttgatcgc caggttgtca gattcggttg caccagaggt aaagacgatt
    31261 tcacgcggat cagcgccgac cagatcggca atctgattac gggcgatatc taccgcttct
    31321 tcagcctgcc agccgaaacg gtgagaacgg gaggccgggt taccaaaggt tccgtccatc
    31381 gtcataaact gcatcatttt ctcggcaaca cgcgggtcca ccggcgtggt tgcggagtag
    31441 tcgagataaa tcggtaattt cattgctcta taaactccgt acatcactca atgcaaggaa
    31501 ccaggctacc ggctggatgt acgaccgtgt ttacggagcg cgatacgccc cggcctgatt
    31561 ctaatatctt gttatcttta acctaactgt ttcgggtttc agcaagttga caaacttacg
    31621 aatctccggg agcttacata agtaagtgac cggagtgagt atgtgcagtc aacgcagatg
    31681 caacacgaaa caggacggtt aaattaagcg cgtaacttaa cgtcgatcgc gtcttgtatg
    31741 cgggtgcgtg gcgcgtcgtg agtatgctga cgaccagaca catccagcac ttcctggtta
    31801 ttaaccagtt cgcctaaagt aatgttgttg agaaaaccgg tgagacggtc gctcaaatca
    31861 cgccacagcg cgtgggtcag gcatttatcg ccgccctggc aaccgccttt accctgacaa
    31921 cgggtagcat ctacagattc gtcaacggcg ctaatcactt cgccaacggc gatgctgctg
    31981 gcatctttgc ctaacagata accaccgcct ggtccacgta cgctggaaac cagaccattt
    32041 ttacgcagac gggaaaacag ttgttccaga taagaaaggg aaattccctg acgttcggaa
    32101 atatcagcca acggtaccgg gcccgcttca gagttgagcg caacgtcaag cattgcggtc
    32161 acggcatagc gccctttaga tgtcagtctc atgtcttact tcacctcaaa ctcgcccctg
    32221 cccggggttt tttattgtaa aagtgggggt attgcatagc agggtcaagt ctgacattcc
    32281 cgagtaaatt ggtcaactat ttacttgact gatttagtcg ggtatttaac cttcagtgcc
    32341 atttttttat cgtggcgttt gtacctgttg tccttccaga ccatgggcgg gttgagggca
    32401 attattcgca ttagcgctgg cctcttcccc tcaccctaac cctctccccg aaggggcgag
    32461 gggaccgtcc gtgcatttat ctggcttagc cctcaggcct tattaccttt attctgctgc
    32521 tctatagaag ccagaatccc gcgcaggata ttcaattcct ggctttccgg gcgcgcacgg
    32581 gtaaacagac ggcgcaattt attcatcacc tgccccggat ggttttcacg gataaaaccg
    32641 gttgccagca gcgtttgttc cagatgacca taaaaacgct ccagatcatc gaccagcgga
    32701 tacggcgtct cttcatgctc gacctgctcg ccattttcct gagttgccag ccaggccata
    32761 cgcacttcat aggcaataac ctgaaccgcc attgccaagt tcagcgaact gtattccggg
    32821 tttgccgcaa tcgcgacatg ataatggcat ttctgcaact cttcattggt caagccgacg
    32881 cgctcacgac caaacaccag cgccaccggg gtatttgccg cttcagcgac gcttttcaga
    32941 ccgcattcgc gcgggtcgag catcggccac ggcagcgtgc gggagcgtgc gctggtgccc
    33001 accaccaggc tacaaccggc taacgcttcg tcgagcgtat cgacgatgtg agcattacca
    33061 atcacatcgc tggcccctgc tgccagggca atcgcctggg agtcgggttt caccagtgga
    33121 ttaaccagcc acagattggt taatcccatt gttttcatgg cacgggcaac agaacccata
    33181 ttgccggtgt gtgacgtctc caccagcaca attcgaatat tttgcagcat tgtctttctt
    33241 catctaaaga ttattcacgc atcttatcat aaaacgaaga cagatgccga tctcgctgct
    33301 atactctgcg ccgttttccc gttctttaac atccagtgag agagaccgat gcatccgatg
    33361 ctgaacatcg ccgtgcgcgc agcgcgcaag gcgggtaatt taattgccaa aaactatgaa
    33421 accccggacg ctgtagaagc gagccagaaa ggcagtaacg atttcgtgac caacgtagat
    33481 aaagctgccg aagcggtgat tatcgacacg attcgtaaat cttactcaca gcacaccatc
    33541 atcaccgaag aaagcggtga acttgaaggt actgatcagg atgttcaatg ggttatcgat
    33601 ccactggatg gcactaccaa ctttatcaaa cgtctgccgc acttcgcggt atctatcgcc
    33661 gttcgtatca aaggccgcac cgaagttgct gtggtatacg atcctatgcg taacgaactg
    33721 ttcaccgcca ctcgcggtca gggcgcacag ctgaacggct accgtctgcg cggtagcacc
    33781 gctcgcgatc tcgacggtac cattctggcg accggcttcc cgttcaaagc aaaacagtac
    33841 gccactacct acatcaacat cgtcggcaaa ctgttcaacg aatgtgcaga cttccgtcgt
    33901 accggttctg cggcgctgga tctggcttac gtcgctgcgg gtcgtgttga cggtttcttt
    33961 gaaatcggtc tgcgtccgtg ggatttcgcg gcaggcgagc tgctggttcg tgaagcaggc
    34021 ggcatcgtca gcgacttcac cggtggtcat aactacatgc tgaccggtaa catcgttgct
    34081 ggtaacccgc gcgttgtgaa agccatgctg gcgaacatgc gtgacgagtt aagcgacgct
    34141 ctgaagcgtt aatgactcag gcgggtgatg tcactcaccc gccctcgcct ttcaggcgct
    34201 attctgaaat acttcctcac cgctttactt tctttcgtca ctctcccacc atctttcccc
    34261 gattaatgga tgataaagaa gtcacaagga aaggttatgg cgctgccagt gaacaaacgc
    34321 gttctcaaaa ttctgtttat tctctttgtt gttgccttct gcgtttattt agtgccgcgc
    34381 gttgccatca acttcttcta ttatcctgac gataaaattt acggtcccga cccctggtcg
    34441 gcggaatccg tcgaatttac ggctaaggac ggtactcgtc tgcaaggctg gtttattcct
    34501 tcttcgacgg gccctgctga caatgccatc gcaaccatca ttcatgctca cggcaatgcc
    34561 ggaaatatgt ccgcccactg gccgctggtc agttggttac ccgagcgtaa tttcaacgtt
    34621 tttatgtttg attatcgcgg gtttggtaaa tcaaaaggca cgccgtccca ggccggattg
    34681 ctggacgata cgcaaagtgc catcaatgtg gtgcgccatc gcagtgatgt aaacccacaa
    34741 cggctggtgc tgttcgggca gagtattggc ggggcgaata ttcttgctgt tattggtcag
    34801 gatgatcgtg aaggcatacg tgcggtgatc ctcgactcca catttgcctc ttatgcaacc
    34861 atcgccaatc agatgatccc cggcagtggc tacttacttg atgagagtta cagcggcgaa
    34921 aattatatcg ccagcgtcag cccgatcccg cttttactca ttcacggtaa agctgatcac
    34981 gttatcccat ggcagcacag cgaaaagttg tatagcctgg caaaagagac aaaacggttg
    35041 atcctaatcc cggatggcga acacattgat gctttttccg atcgtcacgg cgatgtttat
    35101 cgcgaacaga tggtggactt tatccttagc gcgttgaatc cgcagaacta acccatgatc
    35161 gctagcacga taatcattca caaaaccacc ttaagacatg ctaatccact ggtcagaaca
    35221 gtttaagatg agaaaaattc tgtgacgctt gccaacattt ctgatgatta gcattccctt
    35281 cgccatctcc ctgagcaaac tttagctatt cttatcaatt atgcttatgg gagatctaca
    35341 gatgatgcct acgcttgctc caccatctgt cctttcggct ccccagcgcc gctgccagat
    35401 cttgctgacg ctctttcagc cggggttgac cgccaccatg gcaaccttca gcgagcttaa
    35461 tggtgtggat gatgatattg ccagtcttga tatcagcgaa acaggacggg agatcctgcg
    35521 ctatcatcaa ctcacactga cgactggtta tgacggtagc taccgggttg aaggtacagt
    35581 gcttaaccaa cgtttgtgtt tatttcactg gctacgacgt ggtttccgtc tgtgtccgtc
    35641 atttattacc agccaattca cccccgccct gaagagtgaa ctgaagcggc gcggaattgc
    35701 gcgtaacttt tacgacgata ccaatctaca agcgttagtg aatctctgct cccgacggct
    35761 gcaaaaacgc tttgaaacgc gcgatattca tttcctgtgt ctgtatctgc aatattgttt
    35821 gctgcaacac cacgctggaa ttacgcctca gtttaatccg ctccaacgtc gctgggcgga
    35881 atcctgcctt gaatttcagg tagcgcagga aattggacgc cactggcagc gtcgggcgct
    35941 ccagcctgta ccgcctgatg agccactgtt tatggcatta cttttttcca tgttgcgggt
    36001 tcccgatcca ttgcgtgatg cgcatcagcg agacagacaa ttgcgtcagt ctatcaaacg
    36061 tctggtaaac cattttcgtg agctgggaaa tgttcgtttt tatgatgaac aggggttatg
    36121 cgatcagctt tatacccacc tcgcccaggc gttaaatcgc agtttgtttg ccatcggtat
    36181 tgataatacc ctgccggaag agttcgccag actgtaccca cgcctggtgc gcaccacccg
    36241 cgcggcgctg gccggatttg aaagtgaata cggcgtccat ctttctgatg aggaaagcgg
    36301 tctggtcgcg gtgattttcg gtgcctggct aatgcaggaa aacgatctgc atgaaaaaca
    36361 gattattcta ctgaccggga atgatagcga gcgagaagcg cagattgagc agcagttacg
    36421 cgaactaacg ttactgccgc tcaacattaa gcatatgtcg gtaaaggcat ttttgcagac
    36481 aggcgctccg cgtggcgcgg cactgattat tgcgccttat accatgccat taccgctctt
    36541 ttcaccaccg ctgatctata cggacctgac gctgacaaca catcaacagg agcagatccg
    36601 caaaatgctc gaatcagcat gagggaacaa ctttcgggcg caaaaacatt gccggaagcg
    36661 ccaccagcgc cattacccag aacacgccgt ggcccagata ttgatacagg aaaccggcga
    36721 aaacggtcat gatagcgata ctgccgccca tcgcgacggc agagtaaacc gcctgtaaac
    36781 ggatgacttc gctaccctgg cgaacagcaa tatagcgcat ggcggccagg tggcagaccg
    36841 tgaaggtgcc gcaatgcaaa atttgcacca ctatcaacca cggcaacgcc gtagttgctc
    36901 ccataatgcc ccagcgcact acgccgcaaa tcgccgagat caacagcata tcgcgagcac
    36961 tacaacggcg gaaaagttta ttactcagcg caaagataat gacttccgcc accacgccca
    37021 gcgaccacaa ataccccacc gccgaggccg agtagccagc tgcctgccag taaatggcgc
    37081 taaaaccgta ataggccgaa tgtgccccct gcaataaaca aacgcaggcc agaaagcgcc
    37141 agttctggcg aaccagcgcc aaccacgcag accaaccggt gctctcctgc tggcgacttg
    37201 ccccttgtgg ctgaatcgtc ggacggatga gaaagccgag cagcatggat gccacgccca
    37261 acgtcaacag cgcgaggatc acccgataat caaacatagt gaccagtttg cccgtcagcg
    37321 ccgagccaat gacaaacgcc accgagcccc acagtcgcac tttgccgtaa tcaagcggga
    37381 actgcttttg ccacgtattc gccagtgcat cggtcaacgg taccagcggt gagaaaaaga
    37441 ggttaaagcc aatcatcacc agcatcagcc acgctacgtg cgcccccgcc cagaaggcga
    37501 cagcaaagag aagtgtcagc agtgccagca cgcgcaaggc ggaaatcagg cgggaaggat
    37561 cgctgacgcg gggcgcgatg agcaaactcc cgagaaaacg ggcaaccaga cctgccccca
    37621 ataacaggcc gatggtttct ggcgttaaac caatcccttt aagccagacg ctccagaaag
    37681 gtagaaaaat gccgtaacta aaaaagtatg tgaaatagcc gagcgccaac cagcgcgtgg
    37741 attgcaaaac catgagtccc tcccgtcagg aggcgttagt ctggggttaa tcgaatgttg
    37801 gtgcaagttg aaaaataaga atattcgagc ctgcttcccg atgtctgtaa cgccagttca
    37861 caagcactaa aaaactgcga tccgcgacgc atgttgcaaa aataatgcgt aattatgccg
    37921 ttacgcttgc caaacgttcc tgcacaatgg cgatgaaatc gcgtaatgcc ggcttcatct
    37981 cccctttctt ccacgccatc agtaaagcaa tggaaggcac atttccggca atgggacgaa
    38041 aaacaacctg cccggtatta aaattattca tataaccggg tatcaaagtg acgcccagcc
    38101 ccatgcccac cagattcatg gtcaccagaa tattcgttgc cacctggacg atatttggct
    38161 ggctgttttc ttgcgcaaac cacgctttaa cgatcggcgc aagcgaaccg gaatacgccg
    38221 gatcggtact gacgaaattc acgccatcca gttgcgcggc ggtgatctct ttttcatgtg
    38281 ctaaagggtg atcaaccggt aacaccacca ctaatggttc gtcaaaaagc tccagataat
    38341 caatctccgg gctataaaca ggatggcgca ttaagccgac gtcgagttca ccacggcgaa
    38401 ttttttcctc ctgttgcgtg gtgattaaac tcaccagctc aatcaaggtg tctggctgtc
    38461 tgagacgaaa cattggtaat acttttggca ataaattcac ttccgccgat ggcacaaagc
    38521 caatggttaa ttgtctgtct tcctgaacaa ttttccgcgc ccgtaatttg gcattttccg
    38581 cttgttcgag gattgccagc gcgtcctgga gaaaacattc tcccgccgcc gtcaacgcga
    38641 ctttgcgctt atccctcacc agtaacggaa caccgacaca gttttcaaga tcgcggatct
    38701 ggctgcttaa cgaaggctgt gaggtatgca gtttttccgc cgcacgggta aagttcagtg
    38761 cctgcgccac tgcgacgaaa tagcgtaaat gccgtagttc catcaccttc cccttgttat
    38821 cgaaaaaacg tctcagctgg tagaaattaa ctatttcaca tattagcaac caaccagcaa
    38881 catccttatg acacaaaaat agaaggtcaa tacatcttat ctttcaggat taaaaaatat
    38941 gaccacaccc tcagatttaa acatttacca actgattgat acccaaaatg gtcgggtcac
    39001 tccgcgtatt tataccgacc cagatattta ccaactggag cttgaacgta ttttcggccg
    39061 ttgctggtta tttctcgccc acgaaagcca gatcccaaaa cctggtgatt tctttaacac
    39121 ctacatgggg gaagatgcgg ttgtcgtagt gcgtcagaaa gacggcagca ttaaggcgtt
    39181 tctcaaccaa tgccgccacc gggccatgcg tgtgagttat gcagattgcg gcaacactcg
    39241 cgcctttacc tgtccgtatc acggctggtc ttatggcatt aacggcgagt tgatcgatgt
    39301 accgctggaa cctcgcgcct acccacaagg gttgtgtaaa tcccactggg gactaaacga
    39361 agttccttgt gtggagagtt ataaagggct gatttttggc aactgggata ccagcgcacc
    39421 gggcctgcgt gattacctgg gtgacattgc ctggtatctg gatggcatgc tggatcgtcg
    39481 cgaaggcggc accgaaattg tcggcggcgt acaaaagtgg gtgatcaact gtaactggaa
    39541 attcccggca gagcagttcg ccagtgacca gtatcatgct ctgttcagcc atgcttctgc
    39601 cgttcaggta ttaggggcga aagatgatgg cagcgataag cgcctcggtg atggacaaac
    39661 cgcccgcccg gtgtgggaaa ccgccaaaga tgcgctgcaa tttggtcagg acggtcacgg
    39721 tagcggtttc ttctttactg aaaaaccgga tgctaatgtc tgggtcgatg gcgcagtttc
    39781 aagctattac cgcgaaacct atgccgaagc agaacaacgt ttaggtgaag ttcgcgccct
    39841 gcgcctggcg ggtcataaca atattttccc cacgctttca tggctcaacg gcactgccac
    39901 gctccgcgtc tggcatccgc gcggccctga tcaagttgaa gtgtgggcgt tctgtattac
    39961 tgacaaagcc gcctccgatg aagttaaagc cgcttttgaa aacagcgcca ctcgtgcttt
    40021 tggtcctgct ggttttctcg agcaggatga ctcggagaac tggtgtgaaa tccagaaatt
    40081 gcttaaaggc caccgcgccc gcaacagcaa actgtgtcta gaaatggggc ttggtcagga
    40141 aaagcgccgc gacgacggca ttcctggcat tactaactat atcttttcag aaactgccgc
    40201 tcgtggaatg taccaacgct gggccgatct tctgagtagc gaaagctggc aggaagtgct
    40261 cgataaaacc gccgcttacc agcaggaggt gatgaaatga gtgcgcaagt ttcactagag
    40321 ttacatcacc gcattagcca gtttctcttt cacgaagcca gcttactgga cgactggaaa
    40381 tttcgtgact ggctggagca gctcgacaaa gagattcgtt acaccatgcg caccacagtt
    40441 aacgcgcaaa cacgcgaccg ccgcaaaggc gtccagccac cgacaacctg gatttttaat
    40501 gacaccaaag accagctgga gcggcgaatc gcccgtctgg aaacgggcat ggcctgggca
    40561 gaagagccgc cgtcacgcac ccgtcactta atcagcaact gccagataag cgaaaccgac
    40621 atcccaaacg tatttgctgt gcgggtaaat tatctgcttt atcgggcaca aaaagagcgc
    40681 gatgaaacat tctatgttgg aacgcgtttc gacaaagttc gccgtctgga agatgacaac
    40741 tggcgcttgc tggaacggga tatcgtcctg gatcaagcgg taatcacttc ccataacctg
    40801 agtgtactgt tctgatgaat cgaatttatg cgtgtcccgt tgcggatgtg ccggagggtg
    40861 aggctctccg gatcgatacc tcgcccgtca tcgccctgtt caacgttggc ggcgagtttt
    40921 atgccattaa cgatcgttgc agccatggta atgcgtcgat gtcagaaggg tatctggaag
    40981 atgacgccac ggtggagtgc ccgctacacg ccgccagttt ttgcctgaaa acggggaaag
    41041 cgttatgcct gcccgccacc gatccgctca ccacttatcc agtacacgtt gaaggtggtg
    41101 acattttcat cgacttaccg gaggcgcagc catgagcgat ctgcataacg agtccatttt
    41161 tattaccggc ggcggatcgg gattagggct ggcgctggtc gagcgattta tcgaagaagg
    41221 cgcgcaagtt gccacgctgg aactgtcggc ggcaaaagtc gccagtctgc gtcagcgatt
    41281 tggcgaacat attctggcgg tggaaggtaa cgtgacctgt tatgccgatt atcaacgcgc
    41341 ggtcgatcag atcctgactc gttccggcaa gctggattgt tttatcggca atgcaggcat
    41401 ctgggatcac aatgcctcac tggttaatac tcccgcagag acgctcgaaa ccggcttcca
    41461 cgagctgttt aacgtcaacg tactcggtta cctgctgggc gcaaaagcct gcgctccggc
    41521 gttaatcgcc agtgaaggca gcatgatttt cacactgtca aatgccgcca ggtatcctgg
    41581 cggcggtggc ccgctgtaca ccgccagtaa acatgccgca accggactta ttcgccaact
    41641 ggcttatgaa ctggcaccga aagtgcgggt gaatggcgtc ggcccgtgtg gtatggccag
    41701 cgacctgcgc ggcccacagg cgctcgggca aagtgaaacc tcgataatgc agtctctgac
    41761 gccggagaaa attgccgcca ttttaccgct gcaatttttc ccgcaaccgg cggattttac
    41821 ggggccgtat gtgatgttgg catcgcggcg caataatcgc gcattaagcg gtgtgatgat
    41881 caacgctgat gcgggtttag cgattcgcgg cattcgccac gtagcggctg ggctggatct
    41941 ttaaggaagc acgatgaaag aaaaaacgat cattattgtc ggtggcgggc aagcggcggc
    42001 aatggctgcg gcctcgctac gccagcaagg gttcaccggt gagctgcatc tgttttccga
    42061 tgagcgacat cttccttatg aacgacctcc actctcgaaa tccatgttgc tggaagattc
    42121 cccgcagtta cagcaggtgt tacccgctaa ctggtggcag gaaaacaatg ttcatctgca
    42181 ttccggtgta accatcaaaa cgctgggtcg cgacacacga gagttagtgt taaccaacgg
    42241 cgaaagctgg cactgggatc agctttttat agcaatcggc gcggcagctc gaccgctgcc
    42301 gttgcttgat gcactgggag aacgctgctt taccctacgc catgccggtg atgccgccag
    42361 actgcgagaa gttctgcagc ccgaacggtc agtcgtgatt gtcggtgccg gaactattgg
    42421 tctggaactg gctgccagcg ccacgcagcg cagatgtaag gtgacagtga ttgaactggc
    42481 ggcaaccgtc atgggccgta atgcaccacc gcccgtgcaa cgctatcttt tacagcgcca
    42541 ccagcaggct ggtgtgcgca ttctgctcaa taatgccatt gaacatgtgg tcgatggtga
    42601 aaaagtagaa ctgacgctgc aaagtgggga aacgcttcag gctgatgtgg tgatttacgg
    42661 tattggtatc agcgccaacg agcaactggc tcgcgaggcc aaccttgata ctgccaatgg
    42721 cattgtcatt gatgaggctt gccgcacctg cgatcccgcg atctttgccg gtggcgatgt
    42781 ggcaatcact cgtcttgata atggtgcact acaccgctgc gaaagctggg aaaacgccaa
    42841 taaccaggcg caaattgccg ctgccgcaat gttagggcta ccgctaccgc tactgccgcc
    42901 gccgtggttc tggagcgatc agtacagtga taacttacag tttattggcg atatgcgtgg
    42961 cgatgactgg ctttgtcgtg gcaacccgga aactcagaag gcgatttggt ttaatctgca
    43021 aaacggcgtg cttatcggtg cggtcacgct gaatcagggg cgtgagattc gcccaattcg
    43081 caaatggatc cagagcggca aaacgtttga tgcgaaactg ctgatagatg agaacatcgc
    43141 gcttaaatca ctgtaaccag gataattagc gaatatctca acgcctgggg cgtggcgagg
    43201 tgcaagagtg tgtattacgt ttaaatcaca ttatcttgca aagggattgg ttatgaacac
    43261 actacgt
//
LOCUS       DAZPDU010000013        42925 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZPDU010000013 DAZPDU010000000
VERSION     DAZPDU010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42925
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.013"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            38..1645
                     /locus_tag="ACHURS_000644"
     CDS             38..1645
                     /locus_tag="ACHURS_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR1236456.1"
                     /translation="MKVFHNIFKHFSSNHQDKHSDKVNSHQHHGKVDKKYRSNTVEVN
                     MLNDFSQIDNDLGLNIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKSDITYKVNGLS
                     IIPENFFTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNAN
                     TAYFGDTDGRVGAVLYALLVSGHIGIREKGWSLLCELLKHENMASSAYIHKNNKLLYN
                     LLNTRDMILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMDG
                     NKDSRINKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCHYDSETKVL
                     TSHHGIIRNEENECYCLGALQVPFNQMKDPIDPEELANIFNKKHKQHMDDRLIHLIRS
                     NAVASTPVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKYGHYGLGVDQHQKIDNYTM
                     GLNSWKIAPNERGDKKGVPGLSCFQPQ"
     gene            1907..3529
                     /locus_tag="ACHURS_000645"
     CDS             1907..3529
                     /locus_tag="ACHURS_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR1236457.1"
                     /translation="MKIGTVAGTNDSTTTITTNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTINDNANDVAVTESYKPIWNWK
                     ISQYNVFDIKFETMIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNIIISILNRRDIVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDKFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYSGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSSDEMTNALDPVELAAIINKKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            3719..3832
                     /gene="yhiY"
                     /locus_tag="ACHURS_000646"
     CDS             3719..3832
                     /gene="yhiY"
                     /locus_tag="ACHURS_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFR1236458.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            3895..4962
                     /locus_tag="ACHURS_000647"
     CDS             3895..4962
                     /locus_tag="ACHURS_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR1236459.1"
                     /translation="MDKSKRHLAWWVVGLLVVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQSELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            4959..7694
                     /gene="rbbA"
                     /locus_tag="ACHURS_000648"
     CDS             4959..7694
                     /gene="rbbA"
                     /locus_tag="ACHURS_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFR1236460.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKNVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPRHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRSQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            7694..8818
                     /locus_tag="ACHURS_000649"
     CDS             7694..8818
                     /locus_tag="ACHURS_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1236461.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMAKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            8891..9163
                     /locus_tag="ACHURS_000650"
     CDS             8891..9163
                     /locus_tag="ACHURS_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFR1236462.1"
                     /translation="MRTKVQALRKKKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIK
                     EGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            9160..9519
                     /locus_tag="ACHURS_000651"
     CDS             9160..9519
                     /locus_tag="ACHURS_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFR1236463.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(9639..10040)
                     /gene="nikR"
                     /locus_tag="ACHURS_000652"
     CDS             complement(9639..10040)
                     /gene="nikR"
                     /locus_tag="ACHURS_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFR1236464.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(10046..10852)
                     /gene="nikE"
                     /locus_tag="ACHURS_000653"
     CDS             complement(10046..10852)
                     /gene="nikE"
                     /locus_tag="ACHURS_000653"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFR1236465.1"
                     /translation="MTLLNVSGLSHHYAHGGFNGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPAQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(10849..11613)
                     /gene="nikD"
                     /locus_tag="ACHURS_000654"
     CDS             complement(10849..11613)
                     /gene="nikD"
                     /locus_tag="ACHURS_000654"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFR1236466.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKAVYPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            complement(11613..12446)
                     /gene="nikC"
                     /locus_tag="ACHURS_000655"
     CDS             complement(11613..12446)
                     /gene="nikC"
                     /locus_tag="ACHURS_000655"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFR1236467.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(12443..13387)
                     /gene="nikB"
                     /locus_tag="ACHURS_000656"
     CDS             complement(12443..13387)
                     /gene="nikB"
                     /locus_tag="ACHURS_000656"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFR1236468.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(13387..14961)
                     /gene="nikA"
                     /locus_tag="ACHURS_000657"
     CDS             complement(13387..14961)
                     /gene="nikA"
                     /locus_tag="ACHURS_000657"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR1236469.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(15072..15659)
                     /gene="acpT"
                     /locus_tag="ACHURS_000658"
     CDS             complement(15072..15659)
                     /gene="acpT"
                     /locus_tag="ACHURS_000658"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFR1236470.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(15714..16763)
                     /locus_tag="ACHURS_000659"
     CDS             complement(15714..16763)
                     /locus_tag="ACHURS_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFR1236471.1"
                     /translation="MEIPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            16895..18112
                     /locus_tag="ACHURS_000660"
     CDS             16895..18112
                     /locus_tag="ACHURS_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1236472.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVNY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKVGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(18116..18673)
                     /gene="dcrB"
                     /locus_tag="ACHURS_000661"
     CDS             complement(18116..18673)
                     /gene="dcrB"
                     /locus_tag="ACHURS_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFR1236473.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(18746..19411)
                     /locus_tag="ACHURS_000662"
     CDS             complement(18746..19411)
                     /locus_tag="ACHURS_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFR1236474.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSILGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNILLKRLADKSEINAL
                     QAS"
     gene            19632..19877
                     /gene="tusA"
                     /locus_tag="ACHURS_000663"
     CDS             19632..19877
                     /gene="tusA"
                     /locus_tag="ACHURS_000663"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFR1236475.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(19979..22177)
                     /gene="zntA"
                     /locus_tag="ACHURS_000664"
     CDS             complement(19979..22177)
                     /gene="zntA"
                     /locus_tag="ACHURS_000664"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFR1236476.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(22251..22877)
                     /locus_tag="ACHURS_000665"
     CDS             complement(22251..22877)
                     /locus_tag="ACHURS_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFR1236477.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIIRSLYL"
     gene            23018..23377
                     /locus_tag="ACHURS_000666"
     CDS             23018..23377
                     /locus_tag="ACHURS_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFR1236478.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(23380..23649)
                     /locus_tag="ACHURS_000667"
     CDS             complement(23380..23649)
                     /locus_tag="ACHURS_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFR1236479.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(23639..24235)
                     /gene="rsmD"
                     /locus_tag="ACHURS_000668"
     CDS             complement(23639..24235)
                     /gene="rsmD"
                     /locus_tag="ACHURS_000668"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFR1236480.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            24385..25881
                     /gene="ftsY"
                     /locus_tag="ACHURS_000669"
     CDS             24385..25881
                     /gene="ftsY"
                     /locus_tag="ACHURS_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFR1236481.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVKAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            25884..26552
                     /gene="ftsE"
                     /locus_tag="ACHURS_000670"
     CDS             25884..26552
                     /gene="ftsE"
                     /locus_tag="ACHURS_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFR1236482.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSHRMLTLSDGHLHGG
                     VGHE"
     gene            26545..27603
                     /gene="ftsX"
                     /locus_tag="ACHURS_000671"
     CDS             26545..27603
                     /gene="ftsX"
                     /locus_tag="ACHURS_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFR1236483.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            27848..28702
                     /gene="rpoH"
                     /locus_tag="ACHURS_000672"
     CDS             27848..28702
                     /gene="rpoH"
                     /locus_tag="ACHURS_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFR1236484.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            28973..30076
                     /gene="livJ"
                     /locus_tag="ACHURS_000673"
     CDS             28973..30076
                     /gene="livJ"
                     /locus_tag="ACHURS_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFR1236485.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(30275..30658)
                     /gene="panM"
                     /locus_tag="ACHURS_000674"
     CDS             complement(30275..30658)
                     /gene="panM"
                     /locus_tag="ACHURS_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFR1236486.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWIADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            31082..32191
                     /gene="livK"
                     /locus_tag="ACHURS_000675"
     CDS             31082..32191
                     /gene="livK"
                     /locus_tag="ACHURS_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFR1236487.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            32239..33165
                     /gene="livH"
                     /locus_tag="ACHURS_000676"
     CDS             32239..33165
                     /gene="livH"
                     /locus_tag="ACHURS_000676"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFR1236488.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            33162..34439
                     /gene="livM"
                     /locus_tag="ACHURS_000677"
     CDS             33162..34439
                     /gene="livM"
                     /locus_tag="ACHURS_000677"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFR1236489.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDSASDVR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVS
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            34436..35203
                     /gene="livG"
                     /locus_tag="ACHURS_000678"
     CDS             34436..35203
                     /gene="livG"
                     /locus_tag="ACHURS_000678"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFR1236490.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRNQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            35205..35918
                     /gene="livF"
                     /locus_tag="ACHURS_000679"
     CDS             35205..35918
                     /gene="livF"
                     /locus_tag="ACHURS_000679"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFR1236491.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            36202..37266
                     /gene="ugpB"
                     /locus_tag="ACHURS_000680"
                     /pseudo
     CDS             36202..37266
                     /gene="ugpB"
                     /locus_tag="ACHURS_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
     gene            37364..38251
                     /gene="ugpA"
                     /locus_tag="ACHURS_000681"
     CDS             37364..38251
                     /gene="ugpA"
                     /locus_tag="ACHURS_000681"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFR1236492.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSRQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALHSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            38248..39093
                     /gene="ugpE"
                     /locus_tag="ACHURS_000682"
     CDS             38248..39093
                     /gene="ugpE"
                     /locus_tag="ACHURS_000682"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFR1236493.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            39095..40165
                     /gene="ugpC"
                     /locus_tag="ACHURS_000683"
     CDS             39095..40165
                     /gene="ugpC"
                     /locus_tag="ACHURS_000683"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFR1236494.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIVLPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            40162..40905
                     /gene="ugpQ"
                     /locus_tag="ACHURS_000684"
     CDS             40162..40905
                     /gene="ugpQ"
                     /locus_tag="ACHURS_000684"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFR1236495.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(40892..41332)
                     /locus_tag="ACHURS_000685"
     CDS             complement(40892..41332)
                     /locus_tag="ACHURS_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFR1236496.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            41452..>42925
                     /gene="ggt"
                     /locus_tag="ACHURS_000686"
     CDS             41452..>42925
                     /gene="ggt"
                     /locus_tag="ACHURS_000686"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFR1236497.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGMGIVAGESGILLNNQMDDFSAKPGVPNIYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTV"
BASE COUNT        10260 a        10830 c        11753 g        10082 t
ORIGIN      
        1 ttttgttact aacaagtaaa aatattacag gttatttatg aaagtatttc acaatatatt
       61 taagcacttc tcttcaaacc atcaagataa gcattcagat aaagttaaca gtcatcaaca
      121 ccatggcaag gttgataaaa aatatcgatc aaacacagtt gaagttaata tgctgaatga
      181 cttttcgcaa atagataacg atttaggcct gaatatcatc tatttcctgc aacatggtca
      241 ctggagggta aatgaacata gtcttcagat ggagaaaata tggttttaca atagtgagcc
      301 atctatagat attcaagaat ataatagttt cgccgataat actactaaca cgtttatctt
      361 tacaattata ccagacaata accatgtgat aaaattatcg tctcccatta cagttaagat
      421 tgagtgtaag gatggttatt attttattaa tttctccgga gataaatcag acataattta
      481 taaatcagac ataacttata aagttaatgg gctttctatc atacctgaaa atttctttac
      541 tcttcttagc ggtaatttta aagctgactg gcgttgggat gtctctaaag agacgtttcc
      601 taaggataag tttgatagct atgttaaatc tgtgttttct aaaatagact tttataaaca
      661 atgtggtgtg attaacccac aaaatgccaa cacggcttat tttggcgata cagatggaag
      721 agtaggcgct gtactttatg cattgctcgt ttcagggcat ataggcatca gggaaaaggg
      781 ttggagttta ttatgtgaat tattaaaaca cgaaaacatg gcttcatctg catatataca
      841 taaaaataat aaattgctct acaatttatt aaatacgaga gacatgatat tgaatgagtt
      901 gcatcaacat gtttttttaa aggacgatgc gataacccct tgtatcttcc ttggggatca
      961 tacaggcgat agatttagta ctatttttgg tgataaatat atccttactt tgctaaattc
     1021 tatgcgaaat atggatggta ataaagatag ccgtattaat aaaaatgtca tcgttctggc
     1081 tggaaatcat gaaatcaatt ttaatggtaa ctatactgca cgactagaaa attataaact
     1141 ctctgccggt gatacgtata acttaattaa aacgctcgat gtttgtcatt atgactcaga
     1201 aacgaaagtt ttaaccagtc atcacggaat aataagaaat gaagaaaatg aatgttattg
     1261 tctcggtgct ttgcaggttc catttaatca gatgaaagac ccaattgatc ctgaggaatt
     1321 agcaaatata tttaataaaa aacataaaca acacatggat gatcgtctta tccatctaat
     1381 tagaagcaat gctgtagcat ccacacctgt atatgaaaat tattttaata acactactgc
     1441 tttcagacca aaacctgaag atatcttcaa atgtggacag acgctcaaaa aaactaaaca
     1501 gaaatatggc cattatggat taggggtaga tcagcatcaa aaaattgata attataccat
     1561 ggggctaaat tcctggaaga tagcacctaa cgaacgtggt gataaaaaag gtgtcccagg
     1621 gcttagttgt tttcaacctc aataaaactt catgagtgct gtggtatctc tttttaattt
     1681 tgccagtatt tcataggttt tcttaatcga ttgtagaggc atgttctgat gacgcctcta
     1741 caatctttag ggactttctc tgctatgaga taagaaattt catattatgc tttaaggctg
     1801 aatgatttgt gcaaaatata attattttcc catcgaacat gaggtgtatg tcataagatt
     1861 aaatgttaac tatgtattcc tcgcggtaaa taatatgagg taaaagatga aaattggaac
     1921 tgtggcaggc actaacgaca gtacaacgac aatcacaaca aatgatatgg tgcaagagca
     1981 tgttaccaac ttcactaaag aactgtttgg gtatattgca aatggtatag gtgatgatat
     2041 atcaagtatt gccaggacaa tgctcggtga ggttgtggaa aaaatagatg attggcaaat
     2101 agaacggttc caacaatcta ttcaagatga caaaatttca tttaccatac aaacgaatca
     2161 ttcagaaaaa tatagtatgc tatcgggcat gcgcgctcat attctacgta ggaataactg
     2221 ttatcagttt attgtgacaa ttaatagtaa aaattacggt tgccctcttg ataataccga
     2281 tattaattgg tgcagcattg tttatttact taataatatg acaataaatg ataatgctaa
     2341 tgatgttgca gttactgagt cgtacaaacc catctggaac tggaaaatat cgcaatacaa
     2401 tgtatttgat attaaattcg aaacaatgat aaaaccccag ttcgctgaca gaacctattt
     2461 ctccaattgt tcacctgttg atcccacaag taccagaccg acctattttg gcgataccga
     2521 tggctcagtc ggtgcagttt tgtacgcgct ttttgcaacg ggacatttgg gaatcatggc
     2581 tgaaggagaa aatttcctta gccagttgct taatatagaa gatgaagttc tcaacgtatt
     2641 gctaagggag aactttaatg agcaacttga cactaatgtt aatataataa tcagtatcct
     2701 gaacagaaga gacattgttt tagaaagtct tcagccttat ttggttatta ataaagacgc
     2761 agtcacgcca tgtacgtttc tgggcgacca gactggcgat cgatttagta atatctgtgg
     2821 cgataaattt atcatcgatt tattaaaacg cataatgagt atcaatgaaa acgttcatgt
     2881 attagctgga aatcatgaaa cgaattgcaa tggtaactat atgcagaact tcacgcgcat
     2941 gaaaccactt gatgaggata cctactctgg gataaaagat tatcctgtat gtttctatga
     3001 ccccaaatac aaaataatgg caaatcatca tgggataact tttgatgatc agcgaaaacg
     3061 ttacattata ggaccgataa cagtctctag tgatgaaatg accaatgctc tcgatccggt
     3121 cgaattagcg gcaattatca ataaaaagca ccatgctgta attaatggca aaaagtttaa
     3181 aaatagccgg gctatctctt gtcgatcctt taatcgttat ttttctgttt cgacagatta
     3241 cagacctaaa cttgaagcac tattagcatg ctcacaaatg ttaggtataa atcaagttgt
     3301 tgcacataac ggtaatggtg gaagagaatg tataggtgaa acggggacag ttttaggtct
     3361 taacgcccgt gacagtaaac atgctggaag aatgttcagc atgcataatt gccaaattaa
     3421 ccctggtgca gggcctgaga taaccactcc ctggaagtct taccagcatg aaaaaaacag
     3481 aaatggactt atgcctttga tcagaaggcg cacaatgtta cagctctgaa ccatagacaa
     3541 catgaatatt aatgttacta tctggttgag actgttgata aaacgtaaaa aggaatgctc
     3601 tgttcctgca actgaacagg cgtgaaacgc tatccaacag gatggatacc gttttgtcag
     3661 ccagttttat agatcacact tatgagtatg cgtgtttcat ttacggccag gaatgctgat
     3721 gatgacaaca ttactacccg ttttcactaa gccttccccg ttggcgctca atgctctgcg
     3781 cgctggtcgt atttgtcgtt tccttcttat cccggatgga agaatccgct gaacgtcgtt
     3841 cggcggcagt tgtaacacgc gtcgtttgct tgcagggatt atggcgggtc gattatggat
     3901 aagagtaagc gccatctggc gtggtgggtt gtcgggttac tggtggtggc tgccgtcgtg
     3961 gcgtggtggc tgttgcgccc ggcgggtgtg ccggaaggct ttgctgtcag caatgggcgc
     4021 attgaagcga cggaagtgga tatcgccagc aaaattgccg ggcgtatcga caccattctg
     4081 gtgaaagaag gccagtttgt tcgcgaaggc gaagtgctgg cgaagatgga tactcgcgtg
     4141 ttgcaggagc agcgactgga agccatcgcg caaatcaaag aagcacaaag tgccgttgct
     4201 gccgcgcagg ctttgctgga gcaacggcaa agcgaaactc gtgccgcgca gtcgctggtt
     4261 aatcaacgcc agtccgaact ggactccgtg gctaaacgtc atacgcgttc ccgctcgctc
     4321 gcccaacgag gggctatttc tgcgcaacag ctggatgacg atcgcgccgc cgctgagagc
     4381 gcccgagctg cgctggaatc ggcaaaagct caggtatcgg cttctaaagc ggctatagaa
     4441 gcggcccgta ccaatatcat tcaggcccaa actcgcgtcg aagccgcgca agccactgaa
     4501 cggcgtattg ccgcagatat cgatgacagt gaactgaaag ccccgcgtga cggacgcgtg
     4561 cagtatcggg ttgctgagcc aggggaagtg ctggcggcag gcggtcgggt gctgaatatg
     4621 gtcgatctca gcgacgtcta tatgactttc ttcctgccaa ccgaacaggc gggcacgctg
     4681 aaacttggcg gtgaagcccg gctgatcctc gatgccgcgc cagatctgcg tattccggcg
     4741 accatcagtt ttgtcgccag tgtcgcccag ttcacgccaa aaaccgtcga aaccagcgat
     4801 gaacggctga aactgatgtt ccgcgtcaaa gcgcgtatcc caccggaatt actccagcag
     4861 catctggaat atgtcaaaac cggtttaccg ggcgtagcgt gggtgcgggt gaatgaagaa
     4921 cttccgtggc ctgacgacct cgtggtgagg ttgccgcaat gacgcatctg gaactggttc
     4981 ccgtcccgcc tgtcgcgcaa ctggcgggcg tgagccagca ttatggaaaa aacgttgcgc
     5041 tgaacaatat tactctcgat attccggccc gctgtatggt cgggctgatt ggtccggatg
     5101 gcgtcgggaa gtcgagctta ttgtcgttga tttccggtgc ccgcgtcatt gagcagggca
     5161 atgtgatggt gctgggcggc gatatgcgcg acccgaggca tcgccgcgac gtctgcccgc
     5221 gcatcgcctg gatgccgcag gggctgggca aaaacctcta ccacaccttg tcggtgtatg
     5281 aaaacgtcga ttttttcgct cgcctgttcg gtcacgacaa agcggagcgg gaagtgcgaa
     5341 ttaatgagct gctgaccagc acggggttag caccgtttcg cgatcgtccg gcagggaaac
     5401 tctccggcgg gatgaagcaa aaacttgggc tgtgctgcgc gttaatccac gacccggaac
     5461 tgttgatcct tgatgagcca acaacggggg ttgacccgct ctcccgctcc cagttctggg
     5521 atctgatcga cagtattcgc cagcggcaga gcaatatgag cgtgctggtc gccaccgcct
     5581 atatggaaga ggccgaacgc ttcgactggc tggtagcgat gaatgccgga gaagtgctgg
     5641 caactggcag cgccgaagag ctacggcagc aaacgcaaag cgctacgctg gaagaagcat
     5701 ttataaatct gttaccgcaa gcgcaacgcc aggcgcatca ggcggtagtg atcccaccgt
     5761 atcaacctga aaacgcagag attgccatcg aagcgcgcga tctgaccatg cgttttggtt
     5821 ccttcgttgc cgttgatcac gttaatttcc gcattccacg cggggagatt tttggttttc
     5881 ttggttcgaa cggctgcggt aaatccacca ccatgaaaat gctcaccgga ctgctgcccg
     5941 ccagcgaagg tgaggcgtgg ctgttcgggc aaccggttga tccaaaagat atcgataccc
     6001 gccgtcgggt gggctatatg tcgcaggcgt tttcgctcta taacgaactc accgtgcggc
     6061 aaaaccttga gttacatgcc cgtttgtttc acatcccgga agcggaaatt cccgcaagag
     6121 tggctgaaat gagcgagcgt tttaagctca acgacgttga agatgttctg ccggagtcat
     6181 tgccgctcgg cattcgccag cggctttcgc tggcggtggc ggtgattcat cgcccggaga
     6241 tgttaatcct cgatgagcct acttctggtg tcgatccggt ggcgagggat atgttctggc
     6301 agttgatggt cgatctctcg cgccaggaca aagtgactat cttcatctct acccacttta
     6361 tgaacgaagc ggaacgttgc gaccgcatct cactgatgca cgccggaaaa gtgcttgcca
     6421 gcggtacacc gcaggaactg gttgagaaac gcggagccgc cagtctggaa gaggcattta
     6481 tcgcctattt gcaggaagcg gcagggcaga gcaacgaagc cgaagcgccg cccgtggtac
     6541 acgacaccac ccacgcgccg cgtcagggat ttagcctgcg ccgtctgttt agctacagcc
     6601 gccgcgaagc gctggaactg cgacgcgatc cagtacgttc gacgctggcg ctgatgggaa
     6661 cggtgatcct gatgctgata atgggttacg gcatcagtat ggatgtggaa aacctgcgct
     6721 ttgcggtgct cgaccgcgac cagaccgtca gtagccaggc gtggacactc aacctctccg
     6781 gttcccgtta ctttatcgaa cagccgccgc tcaccagtta tgacgagctt gatcgtcgga
     6841 tgcgtgcggg cgatatcacg gtggcgattg agatcccgcc caatttcggg cgcgatatcg
     6901 cgcgtggtac gcctgtggaa ctcggcgtct ggatcgacgg agcgatgccc agtcgtgccg
     6961 aaacggtaaa aggttacgtg caggcaatgc accagagttg gcttcaggat gtggcgagcc
     7021 gacaatcgac acccgccagc caaagcgggc tgatgaacat tgagacgcgc tatcgctata
     7081 acccggacgt aaaaagcctg ccagcgattg ttccggcggt gatcccgctt ctgctgatga
     7141 tgatcccgtc aatgctaagc gcccttagcg tggtgcggga aaaagagcta gggtcgatta
     7201 tcaaccttta cgtgaccccc accacccgta gcgaattttt gcttggcaaa cagctgccgt
     7261 acatcgcgct ggggatgctg aactttttcc tgctctgcgg cctgtcggtg tttgtgtttg
     7321 gcgtaccgca taaaggcagt ttcctgacgc tcaccctggc ggcgctgctg tatatcatca
     7381 ttgccaccgg aatggggctg ctgatctcca cctttatgaa aagccagatt gccgccattt
     7441 tcggaacggc gattatcacg ttgatcccgg cgacacagtt ttccgggatg atcgatccgg
     7501 tagcttcgct ggaagggcct ggacgttgga tcggcgaggt ttacccgacc agtcattttc
     7561 tgactatcgc ccgcggaacg ttctcgaaag cgctggatct gactgatttg tggcaacttt
     7621 ttatcccgtt gctgatagcc atcccgctgg tgatgggctt aagcatcctg ctgctgaaaa
     7681 aacaggaggg atgatgcgcc atttacgcaa tatttttaat ctgggtatca aagagttgcg
     7741 cagtctgctc ggtgataaag cgatgctgac gctgattgtc ttctcgttta cggtgtcggt
     7801 gtattcgtca gcgaccgtta cgccaggatc gttgaacctc gcgccgatcg ccattgccga
     7861 tatggatcaa tcgcagttat cgaaccggat cgttaacagc ttctatcgtc cgtggttttt
     7921 gccaccggag atgatcaccg ccgatgagat ggatgccgga ctggacgccg gacgctatac
     7981 cttcgcgata aatattccgc ctaattttca gcgtgatgtc ctcgccggac gccagccgga
     8041 tattcaggtg aacgtcgatg ccacgcgcat gagccaggca tttaccggca atgggtatat
     8101 ccagaatatt atcaacggtg aagtgaacag ctttgtcgcg cgctaccgtg ataacagcga
     8161 accgttggta tcgctggaaa cccggatgcg ctttaacccg aacctcgatc ccgcgtggtt
     8221 tggcggggtg atggcgatca tcaacaacat taccatgctg gcgattgtat tgaccggatc
     8281 ggcgctgatc cgcgagcgtg aacacggcac ggtggaacac ttactggtga tgccgataac
     8341 gccgtttgag atcatgatgg cgaagatctg gtcgatgggg ctggtggtgc tggtggtatc
     8401 gggattatcg ctggtgctga tggcgaaagg tgtactgggc gtaccgattg aaggctcgat
     8461 cccgctgttt atgctgggcg tggcgctcag tctgtttgcc accacgtcaa tcggcatttt
     8521 tatggggacg atagcgcgtt caatgccgca actggggctg ctggtgattc tggtgctgct
     8581 gccgctgcaa atgctttccg gtggatccac gccgcgcgaa agtatgccgc agatggtgca
     8641 ggacattatg ctgaccatgc cgacgacaca ctttgttagc ctcgcgcagg ccattctcta
     8701 ccggggtgcc ggattcgaaa tcgtctggcc gcagttcctg acgctgatgg caattggcgg
     8761 cgcgtttttc accattgcgc tgctgcgatt caggaagacg attgggacaa tggcgtaagg
     8821 aagggaaact tgacttcata ctgtgatgtc atactattga tgtcataaag tgaagtcata
     8881 aggacatgcc atgagaacca aggtacaggc tttacggaag aaaaaaaata cattggatca
     8941 aatatttaaa actcctgttc ctcaagggat caagtggtct gatatagagt cactggttaa
     9001 agcattaggc ggagaaatta aggaaggaag aggttcgcgt tgtaagttca tactaaatat
     9061 gagcgttgcg tgtttccatc ggcctcatcc gtcgccagat accgataaag gcgctgtaga
     9121 aagcgtgcgt gactggttac taagtatagg ggtaaaacca tgatcaaact gaagacgcca
     9181 aactctatgg agattgccgg gcaaccagct gttattacct acgtgccaga actgaatgca
     9241 tttcgtggca aattcctggg cttgtcaggt tattgtgatt ttgtctctga cagcattcag
     9301 gggctgcaaa aagaagggga actttccctg cgagagtatc ttgaggattg taaagctgcg
     9361 ggtattgagc cgtatgcgcg tacagaaaag attaagacct ttacgttacg ctatccagaa
     9421 tcgctgagtg aacgccttaa taatgcggct gcgcagcaac aagtctctgt taatacctat
     9481 attattgaaa cactgaatga gcgtttgaat catttataaa ttttagcggc gtacattacg
     9541 cagaatatct gcctttacag gatctatcta tgcaactgaa cttaatttta taaattaagt
     9601 tcagttaata tcttttcttt cctcgtttca gaaataactc aatcttcctt cggcaagcac
     9661 tgcaaatgcc catgccgcac gccgcgctgg gcgataacgt catcggcaaa atgctgcacg
     9721 tcgcccatat cacctttcag cacggcgatt tccagacagt cgtcgtgatt gatatgcaca
     9781 tgcagcgtgg cgacggagag atcgtggtga tggtgctggg tggagacaat gcggctggct
     9841 aagtcgcgtt tttcgtgttc atacacatag gagagcaccg cgaaaccttg cgtgccgtgc
     9901 tgctgagtgg cctcctgggc cagggcgcta cgcagaatat cgcggatagc ttcggaacgg
     9961 ttgttataac cacgacgctg gctcaggctg tccagcgtct ccagtaaatc gtcatcaagc
    10021 gtgatggtga ctcgttgcat ttgcgttaaa ccttttctgt ggtgcgacgg cgcacgggga
    10081 atgcgggtaa taccgcgttt tgtagcacac gtccggcgtc agaggaaaag gttaatttat
    10141 ctcccaccac ctgggtttcg acgatttgtc cgttgtccat caccatcacc cgctgacaga
    10201 agcgttccac caggcgtaag tcgtgggtga tgaacaggca ggcggtgcca aactgttgtt
    10261 gtagcttttt cagcaggcga atgacgcccg cctgtaacac gagatcaagg ttagaaacgg
    10321 cttcatccag aatcagtagt ttcggttcga ccgccagcgc gcgagccagg cagacgcgct
    10381 ggagctggcc gccgcttaac tgcggtgggc gtttgtcgag aacgctgtca tcgagatcga
    10441 ccgccttcag catttcgctg gcgcgcgcca gttgttcgga ttttttcagt gatagcaggt
    10501 ggcgcatcgg ttcacgcagg atctcgcgca cggttttgcg cggattcacg gcgctgatgg
    10561 agtcctgaaa taccatctga atatcgcggc ggaacgcttt acgctgggcg cgattgagtt
    10621 tcgccagcgg ttcgccacgc cagctaatat tcccctgcgc gggcgattct aaacccacca
    10681 gcaaccgcgc gagggtactt ttcccgcagc cgctgcgccc cagcagggcg acggtttcgc
    10741 cgcttttcag ggtcagggaa acgttattca gcaccgcctg atgttgatgt tttccgttaa
    10801 atccaccgtg ggcatagtga tgggaaaggc cggagacgtt aagtaaagtc atgatgccag
    10861 ctccataccg tagagggcga gatgagcgga aaccaggctg cgcgttaccg catgtttggg
    10921 ggcgttaaac agcgtttcta catcgccctg ttcgacaatt ttaccttgtg acatcaccgc
    10981 cacgtcatcc gccagacgcg ccacaacgcc catatcatgg gtgaccagca gcattcccgg
    11041 cgcttgtttt tgcataatgc tttccagcag atcgaggatg cgcgcctgtg ctaccacgtc
    11101 gaggtcggtg gtcggttcat cggcgatgat aaacggtgat tcacacagca ccgccatcgc
    11161 aatcatcatg cgctgcaaca tgccgccgct catctcgaac gggtacagct tcagcacgcg
    11221 cgcggcgttt tccagcccca ccgcttctat ggcagcggta agcgtagcgt catcggcggg
    11281 tttccctaac gccaggcagg tttcccgcgc gtgggtgtgc atggtgtgta gcggattaaa
    11341 ggcgctgcgc gggttctgca tgatggtggc aattttgatg ccgcgcaggg cgcagggata
    11401 aaccgctttg ccatcggcta aaatttcccc cgccgtctgg cgaacgccag cgggcaaaat
    11461 gcccagcgtc gcggcgcagg ttaatgattt cccgctgccg ctaccgccga ctaacgccag
    11521 cacgcgcccg cgttgcaggg ttaacgatac accgtgtacc agcggctgcg cggcctgtag
    11581 cgcgatatta cgtagttcaa tctgttgcgg cattagtgtg cgtgctccgt caccagatga
    11641 ggatccagat gatcgcgcag tgcgtcaccc accaggttaa aggccatcac gctgataaac
    11701 agcgccagcc ccggccagaa catttgcagc ggctgggtcc agatatactg gcgcgcgtcg
    11761 ttaatcatca cgccccattc ggcggtcggc gcggtcacac cgaggccaag gaaagacatc
    11821 cccgcgacgt gcagcatcat atggccgata tccagcgttg ccagcaccag cagcgaaggg
    11881 atcaccgcgc ctgccagatg atcgacaaac acccgcacat ggcccgcacc ggaaagccgt
    11941 gacgccagca caaactcgcg ttggcgtagt gaaatcacca agctgcgcac catgcgtgca
    12001 taccacgccc agtgcgacag ggcgatggcg ataattacgt tggtcagccc ggtgccgagc
    12061 acgccaacca taaagaacga cagaatcgag gtcgggaagg tcataaacat atcggcgacg
    12121 cgcatggtgg cctgatcaac gcgcccgcca atcaacccgg cgctgccgcc aataaccagc
    12181 cctaatgtca gcaccagcag caggcaggcc attaccgaac cgagcgacac gcgggtcgct
    12241 gccatcagcc gcgagaaaat atcgcgacct aagtgatcgg tgcccagcca gtgctgcgca
    12301 tccggcgaaa gcaggcgcga cggcaaatca atcgcctggg ggtcatacgg cagccaccac
    12361 tggctggtga gcgcaatcag cgccagcagg gcgataatga tcagcgccag gcgtaccgac
    12421 cagcgggaag agaggaaaaa gttcacgcgt gcgctccttc atgacgacga atgcgcgggt
    12481 ccagcgcggc gttgagcaaa tcgacaatca aattacagac cacaaaaacc accaccatca
    12541 tcagcgtaaa gcactgaatc accggatagt cacggttaaa aatcgccgac accgcatagc
    12601 gcccgacgcc cggccaggca aagatgtttt cgataatcat cgtcccgcca atcagttcgc
    12661 cgatgtgcat ccccacggcg gtgatcatcg gcagcgaggc attgcgcaga atatggcggc
    12721 gttcggtctg tttgtcgttc aggccgcgca gacgcgccca agtgacgtga cgctgaccgg
    12781 cgacgtccag catactggcg cgcagcagtc gcgcgttaat cgccagcgac ataaaggcaa
    12841 tggaaaccgc aggcaaaatg atgtgctgcc agccgccgta acccatcgcg ggtagccatt
    12901 gcagatacac cgaaaacgcc atcaccagta aaaacgccag ccagaagtta ggcatcgaca
    12961 cgccaagaaa cgcgatgaaa cgtacggcga aatccggcag acggtcgcga tggcgcgccg
    13021 cccagatacc gagcggtacg gaagtgagca gaattaatac cagcgccgca cctgcaagtt
    13081 ccagcgtggc gggcaggaag ttcagcatat cgtccagcac cgggcgttgg ctggcgaatg
    13141 agataccaaa gtcaagatgc agcgccttcc acaaccaggt accgtactgg acgtacagcg
    13201 gctgatccag cccgagcatg gtgcgggtag aggccagcat ctccggcgtc ggcggcaggt
    13261 tagataaacg cagataatcg agcgccgggt cgccggtacc gaggcgcagc atcagaaaaa
    13321 tgatcaccga ggcggcgagc accatcggga tcagcagcag aaagcggcgt aatacgtaac
    13381 gcaacattaa ggtttcaccg gtttaatctg ttcgaacgga atttcggtgg cgatcggcgc
    13441 gtaggggata ttacccagct ccggttttga taccaccatc atcgagatgt agctgatagg
    13501 cagataaacc gcctcgtcat gcagacgggt caaaatgtcg cgatacagcg cctgacgttg
    13561 cgtttcgtca tgggtcgcca gcacttcgcc gatctcttta tcaatcagcg gcttgtcggc
    13621 taatccttgc tgtgcctgga agtcagcgtg cgacggtacg cgcattgaac tcaggaaggc
    13681 gtgtggatcg tatggcgcgc cccaggtgcg gtggaaaatc atgccaaaac gaccgtcgcg
    13741 ctgacgagca tagatactgc tctcttcttc gccaatcagc gagacatctg cgccaatctg
    13801 gcgcatatca gcctgaatga tttccgccat cgatttgctt aacgcatcgg tgccgatgaa
    13861 cgacagttca atgcgcagcg gctgaccatt tttctcgcgg atgtctttgc ccgcaggcag
    13921 cgtccaaccg gctttttcca gcaactcttt cgctttctgc ggatcgtact ggcgcggttt
    13981 caggccgaga ttagcgtaag gcacagaagg ggcaaacagg gtgtcggcga cctgctgggt
    14041 gccatataac gcgttatcaa tcagcgattt tttgtttacc gcgtaattaa gagcttcacg
    14101 tactgccagc tcgttggtgg gggctttggc ggtattgagc gccagcatca cggtttcgat
    14161 cggctgtgac agttgggtgt ggtaagccgg attctggcta aagcgggcga aggtatcgag
    14221 cggtaataac ccttcgtttc cgtacagcag gtcgatatcg ccagtttcaa acgccaccgc
    14281 gcgggtagtc gggtccggga tgacgttaaa ggtgatcttt ttaatcgctg gcttttcgcc
    14341 ccagtagttt tcgttacgga cgaagacatc gtactgattc agtttcgatt cctgcaaaat
    14401 ccacggtccg gtgccaatcg gcgctttaat tccgttcatg gtttcatggt ttttaaactg
    14461 cgagggagcg ataaaacgga aaggacgggg cagggccagt tcttgcagga aaggatagta
    14521 ggcgcttttc agggtaattt gcagctctgt tttactgagt gctttaacat caacaatctg
    14581 gtttgccagc tccagccagg cgtgacgttg acggttatcg agcactgcgc ggaagttttc
    14641 tgccgccgcc tcggcatcga acggttcacc gttggagaat ttcacgtcat cacgcagggt
    14701 gaaggtccag gttttaccat cttctgaatg agtccagctt tttgccagcc acgggatcac
    14761 cgaaccgtct gcctgatatt tcaccaatgg ttcataaacc atgctctggg cgaacatctg
    14821 gttaggcgtg taaaggtgcg ggtttagtgg cccgacattc accggccagg cggtagtgat
    14881 ttcatctggt gcagcggcat ggacgataaa agacgcacaa gccagcagcg caaatagagt
    14941 gcggcggagt gtggagagca tggtaacccc aatggattaa aatagatggc ggaaataagt
    15001 atgacgattt taagtattcg tcatactgat tacctgttct ggatcaatag atgggcagtc
    15061 gggtgggcgg atcagttaac tgaatcgatc cattgcacac tgtcggcggt gagcgtaaag
    15121 ggggtggggg tgcagatcgc aaggcttaaa ttttctagct gacaatggct gactgacagc
    15181 gaggagtgat aggtgctatc tacgctgacg atttgccagg cgctgccgcc acgctgttta
    15241 acgatggctt ctttgcgcgt ccagatgcgc cagaacattt ccagttgctg atcaggatgc
    15301 acggcgtcca tttcagcgtg ttccccgagg ctgaacacgg cgttcgccag ccagcgccag
    15361 ttggcgcgcg ggcgaatcac ttcgatatcg cagccgactt cgccttcatc gctcaacagc
    15421 agagcgatat cgtcaccgct atggcttaag ttgaaccata gcggcgtttc cggcgcgaat
    15481 gcaggtttgc cttgttcgcc atagatgatc tccggtagcg gggaaagcgt gtgcgaaagc
    15541 aatgcacgcc ccgccagcca gcgttcgcgt cgtggacctt gcggtgcttg ctcgcgtaaa
    15601 cccggtggca gtggagctgc gcttaaggtc gaaactttcc ccagaactat ccgatacata
    15661 tcagggccaa cgtttaatgg aaaatgaaag tgcgtatcgt atcacttgtc gcctcatccc
    15721 ggtaaccgac ttttcggtct gcctggcccc agtaaaatcg ccagtttgct accgcctttg
    15781 gtggtttcca tccagatttt acacacactg gttaatggca ccgaaagtag catccctacc
    15841 gggccgagca gccatcccca aattaacaat gaaagaaata ccaccatggt ggacatcccc
    15901 aggcgatggc ccatcatccg tggttctaaa atattgccga tgaccatatg gaccactaaa
    15961 aacaatgcgc cgaccagaat acattcgtaa acaccattaa acagcagcac ctgaatcatt
    16021 ggcggtacgg cggaaattac cgcgccgata ttgggcacgt agttgagcaa aaacgccagt
    16081 actgcccaca tcagcgcaaa ctgcaccccc atcagctcca gccccagcca gacgatgaca
    16141 cctgtccata aactgagtag cgtcttcaat gcaagatagt gcgaaacgcc tttaagtgcg
    16201 cggtgtaatc ctgcgatgtg aatctgtggg ttattcagcg caaaacgcat tttgtaaggg
    16261 acgtggcgca cttcaaacag cataaaaact acggtcatca ccagcaaaag cacgctcgcc
    16321 attgccccgg aaagcccggt cattagcgct gtggtgaagg taaccacttt ttccgagtcc
    16381 atccgctgca gcattcgctc cggcgacata tgcaaattaa gaaaaggcaa catctcctgc
    16441 aatttaaaaa gtttgcgcgt cagctcctta ttaaacttcg gcagcataga gataaattcg
    16501 ttaaacgatg ccgccagtac gccgaccagc gcggttagtg cgatcagcat caccaccact
    16561 acaatcgtaa tggcaacggg gcgttgtact ccccgacgaa taaaccaggt aacgagcggg
    16621 ttgaggacga tggcaaaaaa cagcgccagt aacagctgca caatgatatc tgccgctgcg
    16681 tgaatgcccg cgaggatcac taccagcgag gccagcttga gcagaatgtg catgcccgtt
    16741 ttatcgggtt gagggatttc cattggggct tccttgtgac tttttgtatt aagtgtagcg
    16801 ggagtcacgc cagcaatatt ctgattctta ctgctgattt tcacgtcagc acgtggtaaa
    16861 aatgaaacac tgttgtaaaa atgtggtgat cctcatgccc gaacccgtag ccgaacccgc
    16921 gctaaacgga ttgcgcctga atttgcgcat tgtctccatt gtcatgttta acttcgccag
    16981 ctacctcacc atcgggttgc cgctcgctgt attaccgggc tatgtccatg atgtgatggg
    17041 ctttagcgct ttctgggcag gattggttat cagcctgcaa tatttcgcca ccttgctgag
    17101 ccgtcctcat gccggacgtt acgccgattt gctgggcccc aaaaagattg tcgtcttcgg
    17161 tttatgcggc tgctttttga gcggtctggg atatctgacg gcaggattaa ccgccagtct
    17221 gcccgtcatc agcctgttat tactttgcct gggacgcgtg atccttggga ttgggcaaag
    17281 ttttgccgga acgggatcga ccctgtgggg cgttggcgtg gttggctcgc tgcatatcgg
    17341 gcgagtgatt tcgtggaacg gcattgtcaa ttacggggcg atggcgatgg gtgcgccgtt
    17401 aggcgtcgtg ttttatcact ggggcggctt gcaggcgtta gcgttaatca ttatgggcgt
    17461 ggcgctggtg gccattttgt tggcgatccc gcgtccgacg gtaaaagcca gtaaaggcaa
    17521 accgctgccg tttcgcgcgg tgcttgggcg cgtctggctg tacggtatgg cactggcact
    17581 ggcttctgcc ggatttggcg tcatcgccac ctttatcacg ctgttttatg acgctaaagg
    17641 ttgggacggt gcggctttcg cgctgacgct gtttagctgt gcgtttgtcg gtacgcgttt
    17701 gttattccct aacggcatta accgtatcgg cggcttaaac gtggcgatga tttgctttag
    17761 cgttgagata atcggcctgc tactggttgg cgtggcgact atgccgtgga tggcgaaagt
    17821 cggcgtctta ctggcggggg cagggttttc gctggtgttc ccggcattgg gcgtagtggc
    17881 ggtaaaagcg gttccgcagc aaaatcaggg ggcggcgctg gcaacttaca ccgtatttat
    17941 ggatttatcg cttggcgtga ccggaccact ggctgggctg gtgatgagct gggcgggcgt
    18001 accggtgatt tatctggcgg cggcgggact ggtcgcaatc gcgttattac tgacgtggcg
    18061 attaaaaaaa cggcctccgg aacacgtccc tgaggccgcc tcatcatctt aaaacttact
    18121 gaataaccag cgtattaatg atgttttctg cggtggtctg cgctttttgc tgatcgtcag
    18181 ctggcagagt aatttgcatg gtcagcagtt gattacccac gttacccaga ataacggaag
    18241 agtacgccgt ctggcctttc gcggagataa tactgtctaa ctgctgcatt ttgtgacctt
    18301 tcagctcaat ggctttgttg gttaccactt gcagctgcgg atcgcggcta cgttgctgat
    18361 cttccagacg cttcgccaac accgccagat cttctttcgg atcatcgccc atgatgacga
    18421 tgactgcttt ctgcccggtg gcgtcggacc agacatgcat gttattggcc tgcgttccca
    18481 gcttaccgct ctggtcggtc atatccgctg gcagcgagaa acttaacttg ccatcaagca
    18541 ggttgacggg attcccggta gcgttacttt ccgcgaccga accctgcgcc gtagcgttag
    18601 tgtctttatc atcacaggcc gcaagcccca taaccagcag gccaattccg acatatttaa
    18661 ccagattgcg cattgacttc ttcctttcga taaacggcca taacggctca ttcatccatc
    18721 ttatcacaac tctgataacg aacctttaac tcgcctgcaa agcgttgatt tcggatttat
    18781 ctgccagtct tttcaacaga atattgagta atacgccata cattggcagg aagaaaacga
    18841 tactgattaa cactttgaaa cagtaatcga ccagcgcgat ttccatccag tgttcagcca
    18901 taaaggcatc cgggctacgc cagaaggcaa tgaagaaaaa ggccagcgtg tcgctgacgt
    18961 taccgaacag tgtggacgct gtcggtgcca gccaccagcg gcgactctga cgcaggcggt
    19021 taaaaacgtg cacgtcgagg atctgtccca gcgcgtaggc catgaaactg gcggtggcga
    19081 tacgggcgac aaacaggttg aagtgggcga gtgcgccgaa tccctgccag gaacccatat
    19141 agaatagcga cgagatgacg taggagatca gcaacgccgg gatcatcacc gcaaagatga
    19201 tacgacgggc cagcggtgcg ccaaaaatac gcacggtcag gtcggtagca aggaaaataa
    19261 acgggaagct aaacgcgccc caggtggtat ggaaacccaa aatggagacg ggaagctgaa
    19321 ccagatagtt actggaggtg atcaccagca gatgaaataa cgataaccag aacaacgcct
    19381 tatagcgttg agtttgcgag aaaacgttca tattgtacct ttttgattaa ccattggggt
    19441 gagggaaccc aatacgtacg acacgtctgt taccttaaca taataacgtg cttacccttt
    19501 tttcgagccg ccgcatgata ctgctttacg ttgacaatgc aatggttaat tttcacgcaa
    19561 tcgttaacct ggtttgctta cggactcaca gggcgtaaac tagcgccgtt tttttatgtg
    19621 atgagaagaa aatgaccgat ctcttttcca gccctgacca cacactcgac gcacttggcc
    19681 tgcgctgccc ggaacccgta atgatggtgc gcaaaactgt gcgcaatatg cagcctggcg
    19741 aaacgttgct gattatcgcc gacgatccgg ccactacccg cgatattcct gggttttgta
    19801 cctttatgga acacgaactg gttgcgaaag agacggatgg actgccttat cgttatttga
    19861 ttcgtaaagg cggttgatgg ggaaagattg gcttcgatgc cgccttttcc cctcacccta
    19921 accctctccc cagaggggcg aggggaccga tcgcgctcaa tgttgcgatc ggtttgcctt
    19981 atctcctgcg caacaatctt aacgcattcg ctgtcaccag caccgtcgca cccgtatctg
    20041 ccagcaccgc cagccacagg ccggtcatcc ctaacagcgt ggtgacgagg aagatccctt
    20101 tcagccccag cgcaatggtg atgttctggc ggatattggc gtgagtggcg cgtgccagtt
    20161 caatcatttg caccaggccg cgcaggtggt tatgggttaa tgctgcatca gcggtttcca
    20221 gcgccacgtc tgtgccactg cccattgcaa tcccaatggc ggcagctttc atcgctggcg
    20281 cgtcgttaat accatcaccg accatcgcca ggggcgcatg ttgattcagc ttggtcaccg
    20341 ctttgacttt atcttccggc aataggcccg ctttaaactc cagccccagc tccccggcga
    20401 ttgccgccgc tgcacgtgga ttatcgccgg tgaggatcac tcctttgacc cccagcgcgt
    20461 tcagttcact gatggcagtt gcagcatcgg cgcgcagggt atcctgcaat gcaatgatac
    20521 ccagcacgtc atcattacgt actaccagca caaccgtttg cccggcgctt tccagttcat
    20581 taatcaaacc agcaaatgca tcagcgggat gtttcccggc agcgcaaatc aatacgcgct
    20641 caccgttaac ctgcgcttca atgccagacc cgaccagcgc ccgctgtgat tcggcggtgg
    20701 gaatggcgag ttcagcaacc tgtgcttcgc gtacgatggc ttgcgccagt ggatgcgtcg
    20761 cgccttgctc gaccgccgcc gccagtgtca gcagttcaga ttcactaata cccgttgccg
    20821 gatgaatcgc ggtaacgcgc ggtttaccga cggtcagcgt accggtttta tcaaacgcca
    20881 cctgagtaac acgacctagt tgttccagcg ccgctccgcc tttaatcaac gccccacgac
    20941 gcgctgccgc cgccagcccg gaggtaatcg ccgcaggcgt tgagataact aacgcacacg
    21001 ggcagccaat cagcagcagc gtcagccctt tataaatcca ctcctgccag ctggcggcaa
    21061 acagcagcgg tggcaccagc gtcaccagca gagcgacggc cataatcgcg ggcgtataga
    21121 tacggctgaa acggtcgata aaccgctcaa tgggggcgcg acgctcttcg gcttcttcga
    21181 tcagtttcag aatccggtca atggcgctgg cacccggttc tgacagcact tccagcgtta
    21241 ccagacggtc tacgctggtg gctcctgcag gaactttatc gcccgtcgcg cgctccaccg
    21301 gaatggattc tccggtcagg gcgctttcat caaaactggc aaacggtgag agcagtttac
    21361 cgtcggcagg caaacgccca cctgcggcga cttcaatcac atcgccaggc cgcaggctgt
    21421 taatcgccac ctcttcccgc tcaccgttac gcaggcgcgt ggcggtttct ggtttcagcg
    21481 ccattaacgc gctaacgccc tgacgcgcgc ggctggcggc ccagccttcc agtcgttcac
    21541 caatcaaaaa cagcagcaac accatcgcag cttcagccgt tgcgccaata aacagtgcac
    21601 caatagcggc tacgctcatt aaggtttcaa tggcgaagta gctgccggat ttgatcagcc
    21661 gtaatgcctg acgagcaatc gggtacagcc caaccagcgt ggtcgcgata aacgccagtt
    21721 gcccgaacgg atgattgaac tgctccagac cccagctgat tgccatcatc acgattagtg
    21781 taatcagcgg cagattctct ttcaggcgcg atgcttgtgg ctcgtcggcg gcctgctcat
    21841 cgcgcaggga atagcccgct ttttgcaccg cagattcaac ttgtgcacga atgtcattgt
    21901 cggcatcaac caccagtttt tcggtggcga acaacacctg cacctgattc acacctgcaa
    21961 gctggcgcac ggcattttct accttgcgcg cacaggcggc gcagtccatg ccactgactt
    22021 tccagctata gcgggtgccg gagacgtttt cagagagcgt tggcgagctg gagcatgcgc
    22081 cgtcgcagca acagtcgttg gcgttctgta ccgtggttag cggtttgaac gcagcaaatt
    22141 gaggggcttt cttgccgtga ttgtcaggtg tcgacatggc atactccggt taagtttttt
    22201 ctcattaacc gaaggataca ctctggagtc gactccagag tcaagtttta tcagagatac
    22261 agtgagcgga tgatgaggaa gtgaccggca aagtagcagg ccgcagcaat cgcgttatcc
    22321 gcacggaagc gtcggcgata gtggctcccc agccagacaa agttactgat aaacagcaac
    22381 gaagcgccaa caaacgcaga gagcgccgga gcggtcggac ggaagaacca cagttcacct
    22441 gccagccaca ccatcaccag cgtcatgccg ataaacgtgc agataggcca acgatactct
    22501 tccagccgcg tccagataat cgccagtaac agcgcaccca gaaccagcaa caccagtggc
    22561 agaggccaga agaaagagag cgtcatctga ctggcgaaat agatggtgta cagcaggtgc
    22621 gagaggaaaa acgcgccgat ggcgtacatc agacgttgac gtggcaacag ggttagcgca
    22681 tcgcccagca gtgaggcgca cagccctgcc agcaccagat agctaatggc gtcgaacatc
    22741 ggcgcttgcc aggccagtaa cagcaggaga agaagggtta acggtttaaa cacccagcgt
    22801 tgccaggttg gcccacgata cgatgcatcc acagatagcc atgcggaaag acagacagcg
    22861 ataaacgacc aaagcatctt agttccttga attcgttatt ttttcgtaag cggaatgcct
    22921 acggtcttgc ttccagttta gtggcgaggg tggacggatg acaacaccat aactggcagg
    22981 gtatgcttat ttccgcattt tccgatgagg gataaagatg agcaaaccac ctcttttctt
    23041 tattgttatc attggcttaa ttgtcgtcgc cgcatcgttt cgttttatgc aacagcgacg
    23101 ggaaaaagct gataatgata tggctccgct ccagcaaaag ctggtggtgg tgagcaacaa
    23161 gcgggaaaaa ccgattaacg atcgccgttc gcgccagcag gaagtgactc cggcaggcac
    23221 cagtatgcgc tacgaggcaa gcttcaaacc gcaaagcgga ggaatggagc agacgtttcg
    23281 cctcgacgcc cagcagtacc acgccctgac agtgggcgat aaaggtacgc tgagctataa
    23341 aggaacgcgc tttgttagct ttgttggcga acaataaggt tattttttaa ctttaaattt
    23401 cttctgccag accagcagtt caaacacgcc gaaaaggaaa atccgtacct tttcggcggt
    23461 ggtcatctgc gggccatctt tcggtaaagt ggatttcaat agcgccagct gcataccatg
    23521 catcagcacg gtaaaaatca gcgccacgtt aacgaaaata tttagcgggc gcgggaaggg
    23581 atgcaccaga ttgaggatta aaaatcccca aacgcagagc attaacaaac gaccaatatt
    23641 aatcagcatc actttctcct tgtgcttcgc gttgatacag ccgataagcc acctgacccg
    23701 ccactttttc ccgatgtaac gaccagtttg ccggaacggt gggcagaccg ttttcgactt
    23761 cgctttcgac ataaatcagg gcttcgtcag ccagccagcc gttatcttcc agtaaattta
    23821 tcgtctcttc taacaagcca cggcggaacg gtggatcgac aaacacgata ttatgcggtg
    23881 tgcctttttg cgccaggaat gacatcgcgt tgctgttcac cacgcgtgca ttgcctgctt
    23941 ttagtgtcgc cagattctta attaactgct gagaaactgc acgatccatc tcaattaacg
    24001 tggcccccgc agcgtagcgc gataacgctt ccagccccag cgcgccgctc ccggcgaagc
    24061 aatccagaca ttgggcgtca acaatgaccg gagccagcca gttaaacaac gtttcgcgta
    24121 cgcggtcggt ggtggggcgc agacctgggc tatcaggaac cgggagttta cggcctcgcc
    24181 actgcccgcc aataatgcgg atttggccgc tgccggaatg attcggtttt ttcatgataa
    24241 attgctcaat ccgccagata acacatactt gcaggcggtg atgtgaatta agttaagtga
    24301 tagactattt catcattttt ttagctgcta tgtacatagc gttaacgctg tgccatgaag
    24361 caacagcgag gagtgtagtc gcaaatggcg aaagaaaaaa aacgtggctt tttttcctgg
    24421 ctgggctttg gtcaaaaaga gcagaccccg gaaaaagaga cagaagttca gaatgaacaa
    24481 ccggttgtag aagaaatcgt tcaggcgcaa gagcctgtga aggcttcgga acaagccgtt
    24541 gaagagcagc cgcaggcgca tactgaagcc gaggcggaaa cttttgctgc cgacgttgtg
    24601 gaagtcactg aacaggttgt tgaaagtgaa aaagcgcagc ctgaagcgga agttgttgca
    24661 cagccggaac cggtcgtaga agaaacgccg gagccagtgg ctatcgaacg tgaagagctg
    24721 ccgttgccgg aagacgtcaa cgccgaagag gtttcgccag aagagtggca ggctgaagcg
    24781 gaaaccgtag agattgtcaa agcggcggaa gaagaagcgg ctaaagaaga aatcaccgac
    24841 gaagagctgg aagcacaggc gctggcagcc gaagcagcag aagaggcggt gatggtggtt
    24901 cctccggcag aagaagagca gccggtggaa gaaatcgctc aggagcagga aaaaccaacc
    24961 aaagaaggtt tcttcgcgcg cctgaaacgc agcctgttaa aaaccaaaga aaatctcggt
    25021 tccggattta tcagcctgtt ccgcggtaaa aaaatcgacg atgatctgtt tgaggagctg
    25081 gaagagcagc ttttgatcgc cgatgtgggc gtggaaacca cacgtaaaat tatcaccaat
    25141 ctgacggaag gcgcatcccg caagcagctt cgtgatgccg aggcgctcta tggcctgctg
    25201 aaagaagaga tgggtgagat tctggcgaaa gtcgatgagc cgctgaatgt tgaaggtaaa
    25261 acgccgttcg tgatcctgat ggtgggcgtc aacggtgtgg gtaaaaccac gacgattggt
    25321 aagctggcac gtcagtttga gcagcagggt aaatcggtga tgctggcggc gggcgatact
    25381 ttccgtgcag cagcggttga acagcttcag gtctggggtc agcgcaacaa tattccggtg
    25441 attgcccagc ataccggggc ggattccgcc tctgttatct tcgacgccat tcaggcagct
    25501 aaagcgcgta atatcgacgt cctgattgcc gataccgccg gacgcctgca gaacaaatcg
    25561 cacctgatgg aagagttgaa gaaaatcgtc cgcgtgatga agaaactcga cgttgaagcg
    25621 ccgcatgaag ttatgctgac tattgatgcc agcaccgggc agaacgcggt aagccaggcc
    25681 aaactgttcc acgaagctgt tggcttaacc ggcatcacgc taacgaaact ggacggcacg
    25741 gcgaaaggcg gggtaatttt ctcggtggct gaccagtttg gtatccctat ccgctacatt
    25801 ggtgtcggcg aacgtattga ggatttgcgt ccgtttaagg cggacgactt tatagaggca
    25861 ctttttgccc gagaggatta acaatgattc gctttgaaca tgtcagcaag gcttatctcg
    25921 gtgggagaca ggcgctgcag ggcgttacgt tccatatgca gccgggtgag atggcgtttc
    25981 tgaccggtca ttccggcgca gggaaaagta ccctcctgaa gctgatctgt gggattgagc
    26041 ggcccagcgc cgggaaaatc tggtttagcg gccatgacat cacgcgtctg aaaaaccgtg
    26101 aagttccgtt tctgcgccgc cagattggca tgattttcca ggatcaccat ctactgatgg
    26161 accgtactgt ctacgataac gtggcgatcc cgctgattat cgctggtgcc agcggtgacg
    26221 atattcgtcg ccgggtgtcg gcggcactgg ataaagtcgg gctactggac aaagcgaaga
    26281 acttccctat tcagctttcc ggcggtgaac aacagcgtgt tggcattgcc cgcgcggtgg
    26341 tgaataagcc cgcggtactg ctggcggacg aaccgaccgg taacctggac gacgcgttgt
    26401 cggaagggat tctacgtctg tttgaagagt ttaaccgcgt tggggtaacc gtattgatgg
    26461 caacgcacga catcaacctg atttcacggc gttcccatcg catgctcacc ctgagcgatg
    26521 gtcacttgca tggaggcgtg ggccatgaat aagcgcgatg caatcaatca tattcggcag
    26581 tttggtgggc gtctggatcg cttccgtaaa tcggtcggcg gctcaggcga cggcggtcgt
    26641 aacgcaccga aacgcgcgaa atcctcgcca aaaccggtaa atcgcaaaac caacgttttc
    26701 aacgaacagg tgcgctatgc cttccacggc gcattgcagg atctgaaaag caaaccgttc
    26761 gccacgtttt taacggtgat ggttatcgcc atctccctga cgctgcccag cgtctgttat
    26821 atggtgtaca aaaacgttaa tcaggcggcg acgcagtatt atccgtcacc gcaaatcact
    26881 gtttacttgc aaaaaacgct ggacgatgac gccgctgcgg gcgtggtggc acagttgcag
    26941 gccgagcaag gcgtggagaa agtgaactat ctttctcgtg aagacgcact gggcgagttc
    27001 cgtaactggt ctggttttgg tggtgcgctg gatatgctgg aagaaaaccc gcttccggca
    27061 gtggcggtgg tgatcccgaa actcgatttt caggggacgg aatcactcaa tacgctgcgt
    27121 gatcgtatca cgcagattaa cggcattgac gaagtgcgga tggatgacag ctggtttgcc
    27181 cgtctggcgg cgttgaccgg gctggtcggg cgcgtttcgg cgatgatcgg cgtgttgatg
    27241 gtggcggcag tgttcctcgt catcggcaac agcgtacgtc tgagcatctt tgctcgccgt
    27301 gactccatca acgtgcagaa actgattggt gcgacggatg gtttcatcct tcgcccgttc
    27361 ctgtatggtg gcgcactgct gggattttct ggcgcattgt tgtcattaat tttgtcagaa
    27421 attctggtgc tgcgattgtc atcggcggtt gcggaagtgg cacaggtttt cggaacgaag
    27481 tttgatatca atggtttatc gttcgatgaa tgcctgctat tgctgctggt atgctcgatg
    27541 attggctggg tggcagcgtg gcttgccacg gtacaacatt tacgccactt tacgcctgaa
    27601 taataaaagc gtgttatact ctttccctgc aatgggttcc gtagcaggga aagagatccc
    27661 gttgtctctt cccggtattt catctctatg tcacattttg tgcgtaattt attcacaagc
    27721 ttgcattgaa cttgtggata aaatcactgt ctgataaaac agtggatgat aacctcgttg
    27781 ctcttaagct ctggcacagt tgttgctacc actgaagcgc cagaagatat cgattgagag
    27841 gatttgaatg actgacaaaa tgcaaagttt agctttagcc ccagttggca acctggattc
    27901 ctacatccgg gcagctaacg cgtggccgat gttgtcggct gacgaggagc gggcgctggc
    27961 tgaaaagctg cattaccatg gcgatctgga agcagctaaa acgctgatcc tgtctcacct
    28021 gcggtttgtt gttcatattg ctcgtaatta tgcgggctat ggcctgccac aggcggattt
    28081 gattcaggaa ggtaacatcg gcctgatgaa agcagtgcgc cgtttcaacc cggaagtggg
    28141 tgtgcgcctg gtctccttcg ccgttcactg gatcaaagca gagatccacg aatacgttct
    28201 gcgtaactgg cgtatcgtca aagttgcgac caccaaagcg cagcgcaaac tgttcttcaa
    28261 cctgcgtaaa accaagcagc gtctgggctg gtttaaccag gatgaagtcg aaatggtggc
    28321 ccgtgaactg ggcgtaacca gcaaagacgt tcgtgagatg gaatcacgta tggcggcaca
    28381 ggacatgacc tttgacctgt cttccgacga cgattccgac agccagccga tggcaccggt
    28441 gctctatctg caggataaat catctaactt tgccgacggc atcgaagatg ataactggga
    28501 agagcaggcg gcaaaccgtc tgaccgacgc gatgcaaggt ctggacgaac gcagccagga
    28561 catcatccgc gcgcgctggc tggacgaaga caacaagtcc acgttgcagg aactggctga
    28621 ccgttacggt gtttccgctg aacgtgtacg ccagctggaa aagaacgcga tgaaaaaact
    28681 gcgcgctgct atagaagcgt aatttccgct attaagcaga gaaccctgga tgcgagtccg
    28741 gggtttttgt tttttgggcc tctacaataa tcaatttccc ctccggcaaa acgccaatcc
    28801 ccacgcagat tgttaataaa ctgtcaaaat agctattcca atatcataaa aatcgggtat
    28861 gttttagcag agtatgctgc taaagcacgg gtagccatgc ataaaacgaa ataaagtgct
    28921 gaacaacaac atcacaacac acgtaataac cagaagaatg gggattctca ggatgaacat
    28981 aaagggtaaa gcgttactgg caggatgtat cgcgctggca ttcagcaata tggctctggc
    29041 agaagatatt aaagtcgcgg tcgtgggcgc aatgtccggt ccggttgcgc agtacggtga
    29101 ccaagagttt accggcgcag agcaggcggt tgcggatatc aacgctaaag gcggcattaa
    29161 aggcaacaaa ctgcaaatcg taaaatatga cgatgcctgt gacccgaaac aggcggttgc
    29221 ggtggcgaac aaagtcgtta acgacggcat taaatatgtg attggtcacc tctgttcttc
    29281 atcaacgcag cctgcgtctg acatctacga agacgaaggc attttaatga tcaccccagc
    29341 ggcaaccgcg ccggagctga ccgcccgtgg ctatcagctg atcctgcgca ccaccggcct
    29401 ggactccgac caggggccga cggcggcgaa atatattctt gagaaagtga aaccgcagcg
    29461 tattgctatc gttcacgaca aacagcaata cggcgaaggt ctggcacgag cggtgcagga
    29521 cggcctgaag aaaggcaatg ctaacgtggt gttctttgat ggtatcaccg ccggggaaaa
    29581 agatttctct acgctggtgg cgcgtctgaa gaaagagaat atcgacttcg tttactacgg
    29641 cggttaccac ccggaaatgg ggcaaatcct gcgtcaggca cgcgcggcag ggctgaaaac
    29701 tcagtttatg gggccggaag gtgtggctaa cgtttcgctg tctaacattg cgggcgaatc
    29761 agcggaaggg ctgctggtga ccaagccgaa gaactacgat caggttccgg caaacaaacc
    29821 cattgttgac gcgatcaaag cgaaaaaaca ggacccaagc ggcgcattcg tttggaccac
    29881 ctacgccgcg ctgcaatctt tgcaggcggg cctgaatcag tctgacgatc cggctgaaat
    29941 cgccaaatac ctgaaagcga actccgtgga taccgtaatg ggaccgctga cgtgggatga
    30001 gaaaggcgat ctgaaaggct ttgagttcgg cgtatttgac tggcacgcca acggcacggc
    30061 gacagatgcg aagtaatcat taatcggcaa ctttgggttg ccgccaaatt gctaatatcg
    30121 agtacgttgc ttcatgccgg atgcggcgta aacgccttat ccggcctata agatccaaag
    30181 aaatcagtaa attgcaacac acattgtagg cctgataagc gtagcgcatc aggcaatttc
    30241 tcgttggtca tcacttttga aatcaacttg acgattaacg cttctcccag ccgccctgtt
    30301 gcgccgtaaa tcccagtgcc tgcataaacg ctgtcatcac accgcgatct tccacgcctg
    30361 catccgctat ccaccagcat gaaacgccag gattgttacg caaaacctct tccagcagat
    30421 attgccccac accgcgacgt cgggtgactt cccgcacacg cagggaatcc agtgctccct
    30481 cggtgccgct taaggttacc cgtacggcag cgagcaggcg ctcgttaaaa cgcgcggcgt
    30541 agatacggtg gttatcgtca acctgtaaag aggaagggga atactccggc cagatctttt
    30601 gcaggtcaat ccggtcctgg tcgctaaagt tttctaatcg aatgatggtc agcttcatgg
    30661 gtaacccgtg taaatcacaa aagtggaacc agtgtagcga aataatttaa tcggaggctt
    30721 tctctttttt atttcttttg gcaggtgatt aattttttaa cagcaataat tacaaagtta
    30781 aaacattaga gaatgaaaaa tacccagcat aatcccctga atggtagtga attattccgc
    30841 cctttgtgcc gttattttat gctgacaaag gcactttttt ctgtttatct atcaataaat
    30901 tcagaatatt atctgttctt aatcgactga aaaataggga ttttaatcac tgttatcaca
    30961 aaatactgcg ctaaaccctt aatcagacag gcaaaaacag tgcagtataa aaaaagaaca
    31021 gtctgatttg ttaacacata aaaacaaagc aacacaacat cacgaatggg gatttttgac
    31081 tatgaaacgg aatgcgaaaa ctatcatcgc agggatgatt gcactggcaa tttcacacac
    31141 cgctatggct gacgatatta aagtcgccgt tgtcggcgcg atgtccggcc cgattgccca
    31201 gtggggcgat atggaattta acggcgcgcg tcaggcgatt aaagacatta atgccaaagg
    31261 gggaattaag ggcgacaagc tggttggcgt ggaatatgac gacgcctgcg acccgaaaca
    31321 agccgttgcg gtcgccaaca aaatcgttaa tgacggcatt aaatacgtta ttggtcatct
    31381 gtgttcttct tctacccaac ctgcatcaga tatctacgaa gacgaaggta ttctgatgat
    31441 ctcgccggga gcgaccaacc cggagctgac ccaacgcggt tatcaacaca ttatgcgtac
    31501 tgccgggctg gactcttccc aggggccaac ggcggcaaaa tacattcttg agacggtgaa
    31561 gccccagcgc atcgccatca ttcacgacaa acaacagtat ggcgaagggc tggcgcgttc
    31621 ggtgcaggac gggctgaaag cggctaacgc caacgtcgtc ttcttcgacg gtattaccgc
    31681 cggggagaaa gatttctccg cgctgatcgc ccgcctgaaa aaagaaaaca tcgacttcgt
    31741 ttactacggc ggttactacc cggaaatggg gcagatgctg cgccaggccc gttccgttgg
    31801 cctgaaaact cagtttatgg ggccggaagg tgtgggtaac gcatcattgt cgaatattgc
    31861 tggcgatgct gccgaaggca tgttggtcac tatgccaaaa cgctatgacc aggatccggc
    31921 aaaccagggc atcgttgatg cgctgaaagc agacaagaaa gatccgtccg ggccttatgt
    31981 ctggatcacc tacgcggcgg tgcaatctct ggcgactgcc cttgagcgta ctggcagcga
    32041 tgagccgctg gcgctggtga aagatttaaa agctaacggt gcaaacaccg tgattgggcc
    32101 gctgaactgg gatgaaaaag gcgatcttaa gggatttgat tttggtgtct tccagtggca
    32161 cgccgatggt tcatccacgg cagccaagtg atcatcccac cgcccgtgaa atgcgggcgg
    32221 gtttagaaag gttaccttat gtctgagcag tttttatatt tcttgcagca gatgtttaac
    32281 ggcgtcacgc tgggcagtac ctacgcgctg atagccatcg gctacaccat ggtttacggc
    32341 attatcggca tgatcaactt cgcccacggc gaggtttaca tgattggtag ctatgtctcc
    32401 tttatgatca tcgccgcact gatgatgatg ggcattgata ccggctggct gctggtggcg
    32461 gcgggattcg tcggcgcaat cgtcattgcc agcgcctacg gctggagtat cgaacgggta
    32521 gcctatcgcc cggtgcgtaa ctctaagcgc ctgattgcgc tcatctcggc aatcggcatg
    32581 tccatcttcc tgcaaaacta cgttagcctg accgaaggtt cgcgtgacgt ggcgctgccc
    32641 agtctgttta acggtcagtg ggtggtgggg catagcgaaa acttctctgc ctctattacc
    32701 accatgcagg cagtgatctg gattgttact ttcctcgcca tgctggcgct gacgattttc
    32761 attcgttatt cccgtatggg gcgcgcctgc cgtgcctgcg cggaagatct gaaaatggcg
    32821 agcctgcttg gcatcaacac cgaccgggtg attgctttga cctttgttat tggcgcggcg
    32881 atggcggcag tggctggcgt actgctcggt cagttctacg gcgtcattaa cccctatatc
    32941 ggctttatgg ccgggatgaa agcctttacc gctgcggtgc tcggtgggat tggcagcatt
    33001 ccaggggcga tgattggcgg gctgatcctg gggattgcgg aggcgctctc ttctgcctat
    33061 ctgagtacgg aatataaaga tgtggtctca ttcgccctgc tgattctggt gctgctggtg
    33121 atgccgaccg gtattctggg tcgcccggag gtagagaaag tatgaaaccg atgcatattg
    33181 caatggcact gctctctgcc gcgatgttct ttgtcctcgc tggcgtcttt atgggcgtgc
    33241 aactggagct ggatggcacc aaactggtgg tcgacagcgc ttcggatgtc cgttggcagt
    33301 gggtgtttat cggcacggcg gtggtctttt tcttccagct tttgcgacct gctttccaga
    33361 aagggctaaa aagcgtttcc gggccgaagt ttattctgcc cgcgattgat ggctccacgg
    33421 tgaagcagaa actgttcctc gtggcgctgc tggtgcttgc ggtgtcgtgg ccgtttatgg
    33481 tgtcacgtgg gacggtggat atcgccactc tgaccatgat ctacattatc ctcggtctcg
    33541 ggctgaatgt ggttgttggt ctttctggtc tgctggtgct ggggtacggc ggtttttacg
    33601 ccatcggcgc ttacactttt gcgctgctca atcactatta cggtctgggg ttctggacct
    33661 gtttgccgat tgccggactg atggcagcgg cggcgggctt cctgcttggt tttccggtgt
    33721 tgcgtctgcg cggtgattat ctggcgatcg ttaccctcgg tttcggcgaa attgtgcgca
    33781 tattgctgct caataacacc gaaattaccg gcggcccgaa cggtatcagt cagatcccga
    33841 aaccgacttt cttcggcctt gagttcagcc gtaccgcccg tgaaggcggt tgggacacgt
    33901 tcagtaattt ctttggcctg aaatacgatc cctccgatcg cgtcatcttc ctgtatctgg
    33961 tggcgttgct gctggtggtg ctaagcctgt ttgtcattaa ccgcctgctg cggatgccgc
    34021 tggggcgtgc gtgggaagcg ttgcgtgaag atgagatcgc ctgccgttcg ctgggcttaa
    34081 gcccgcgtcg tatcaagctg actgccttta ccatcagtgc cgcgtttgcc ggttttgccg
    34141 gaacgctgtt tgctgcgcgc cagggctttg tcagcccgga atccttcacc tttgctgaat
    34201 cggcgtttgt actggcgata gtggtgctcg gcggtatggg atcgcagttt gcggtgattc
    34261 tggcggcaat tttgctggtg gtatcgcgcg aattgatgcg tgatttcaac gaatacagca
    34321 tgttaatgct cggtggtttg atggtgctga tgatgatctg gcgtccgcag ggcttgctgc
    34381 ccatgacgcg cccgcaactg aagctgaaaa acggcgcagc gaaaggagag caggcatgag
    34441 tcagccatta ttatctgtga acggcctgat gatgcgcttc ggcggcctgc tggcggtgaa
    34501 caacgtcaat cttgaactgt acccgcagga gatcgtctcg ttaatcggcc ctaacggtgc
    34561 cggaaaaact acggttttta actgtctgac cggattctac aaacccaccg gcggcaccat
    34621 tttactgcgc aatcagcacc tggaaggttt accggggcag caaattgccc gcatgggcgt
    34681 ggtgcgcacc ttccagcatg tgcgtctgtt ccgtgaaatg acggtaattg aaaacctgct
    34741 ggtggcgcag catcaacaac tgaaaaccgg gctgttctct ggcctgttga aaacgccttc
    34801 tttccgtcgc gcccagagcg aagcgctcga ccgcgccgcc acctggcttg agcgcattgg
    34861 tttgctggaa cacgccaacc gtcaggcgag taacctggcc tatggtgacc aacgccgtct
    34921 ggagattgcc cgctgcatgg tgacgcagcc ggagatttta atgctcgacg aaccggcggc
    34981 aggtcttaac ccaaaagaaa ccaaagagct ggatgagctg attgccgaac tgcgcaatca
    35041 tcacaacacc actatcttgt tgattgaaca cgatatgaag ctggtgatgg ggatttcaga
    35101 ccgaatttat gtggtcaatc aggggacgcc actggcaaac ggtacgccgg agcaaatccg
    35161 taataacccg gacgtgatcc gtgcctattt aggtgaggca taagatggaa aaagtcatgt
    35221 tgtcctttga caaagtcagc gcccactacg gcaaaatcca ggcgctgcat gaggtgagcc
    35281 tgcatatcaa tcagggcgag attgtcaccc tcattggcgc gaacggggcg gggaaaacca
    35341 ccttgctcgg cacgttatgc ggcgatccgc gcgctaccag cgggcgaatt gtgtttgatg
    35401 ataaagacat taccgactgg cagacggcga aaatcatgcg cgaagcggta gcgattgtcc
    35461 cggaagggcg tcgcgtcttc tcgcggatga cggtggaaga gaacctggcg atgggcggct
    35521 tttttgccga acgcgaccag ttccaggagc gcataaagtg ggtgtatgaa ctgttcccac
    35581 gtctgcatga gcgccgtgtt cagcgggcag gcaccatgtc cggcggtgag caacaaatgc
    35641 tggcgattgg tcgcgcgctg atgagcaacc cgcgtttgct gctacttgat gagccatcgc
    35701 ttggtcttgc gccgattatc atccagcaaa ttttcgacac catcgagcag ctgcgcgagc
    35761 aggggatgac tatcttcctc gtcgagcaga acgccaacca ggcgctaaag ctggcggatc
    35821 gcggctacgt gctggaaaac ggccatgtgg tgctctccga tactggtgat gcactactgg
    35881 cgaatgaagc ggtgagaagt gcgtatttag gcgggtaata acacgttgat tgataaggag
    35941 tcaaaagact cctttgagac aggtgacaaa tgtaaaattg cctgatgcgc tgcgcttatc
    36001 aggcctactg ggtgagttgc aatatattga atttgcacga tcttgtaggc cggataaggc
    36061 gtttacgccg catccggcat gaaacgatga gtaatctgta gagtttgatt cagaccttct
    36121 atttttccgc ttatccgtgc cccatctccc attttccctc acccacgccg tcaccgcctt
    36181 gtcatctttc tgacacctta cttgcaggtt tatgaagttg gcaccgccac catgatggca
    36241 tcgaaagcca ttaaaccggt atatgacgtg tttaaagagg cagggattca attcgatgag
    36301 tcgcagtttg tgccgacggt ttccggttac tactccgaca gcaaaacggg ccacttactc
    36361 tcccagccat tcaacagctc gactcccgtt ctctattaca acaaagacgc cttcaagaaa
    36421 gcggggttag acccggaaca gccgccgaaa acctggcagg atctggcgga ctcttccgca
    36481 aaactgaaag cctccggtat gaagtgcggc tacgccagcg gctggcaggg ctggatccaa
    36541 ctggaaaact ttagcgcctg gaacggtctg ccgtttgcca gcaaaaacaa cggctttgac
    36601 ggcacggacg cggtgctgga gttcaacaag ccggagcagg tgaaacacat tgccatgctc
    36661 gaagagatga acaagaaggg cgacttcagc tacgtcggtc gtaaggatga atccaccgag
    36721 aagttctata acggtgattg cgcgatgacc accgcctctt ccggttctct cgctaatatt
    36781 cgtgagtacg ccaaatttaa ctatggcgta ggcatgatgc cttacgacgc cgatgcgaaa
    36841 gatgcaccgc aaaacgccat tatcggcggg gccagcctgt gggtgatgca gggcaaaaat
    36901 aaagaaacgt ataccggcgt ggcgaagttc ctcgacttcc tcgcgaagcc agaaaacgct
    36961 gccgagtggc atcagaaaac cggttatctg ccaatcacca aagcagcgta tgacctgacc
    37021 cgtgagcagg gcttttatga gaaaaaccca ggggcggata ccgcgacgcg tcagatgctg
    37081 aataagccgc cgttgccgtt caccaaaggg ctgcgtctgg gcaacatgcc gcagatccgc
    37141 gtgattgtgg atgaagagct ggagagcgtg tggaccggta agaagacacc acagcaggca
    37201 ctggataccg ccgttgagcg tggaaatcag ttgctgcgcc gctttgagaa atcgacgaag
    37261 tcttaatcag tgtaatgtcg gatgcgtttc gcttatctga cctggcatcg cgtgtaggcc
    37321 ggataagcga agcgcatccg gcacagttca ggaattaacc gtaatgtcat catcccgtcc
    37381 ggtgttccgc tcgcgctggc tgccttatct gctggtcgcg ccgcagctca tcatcaccgt
    37441 tatctttttt atctggcctg cgggcgaagc gttgtggtac tcgcggcaaa gcgtcgatcc
    37501 gtttggtttc tccagccagt ttgtcggtct ggataacttc gtcacgctgt tccatgacag
    37561 ctactatcta gactccttct ggacgacgat aaaattcagc acctttgtca ccgtcagcgg
    37621 tttgctggta tcgctgttct ttgcggcgct ggtggagtac atcgtgcgcg gcagccgttt
    37681 ctatcaaacc ttgatgctgc tgccgtatgc cgtggctccc gccgttgccg ccgtattgtg
    37741 gatcttcctg tttaaccccg gtcgcgggct gatcacccat tttctcgctg agttcggcta
    37801 cgactggaac cacgcgcaaa acagcggtca ggcaatgttt ctggtggtgt ttgcctcggt
    37861 gtggaagcaa atcagctaca actttctgtt cttctatgcc gcgctgcact ccattccccg
    37921 ttcgttgatt gaagccgcag ctattgacgg cgcagggccg attcgccgct tctttaagat
    37981 tgctctgccg cttatcgctc cggtgagttt cttcctgctg gtggtgaacc tggtgtacgc
    38041 cttcttcgac accttcccgg tgatcgacgc cgccacgtcc ggcgggccgg ttcaggccac
    38101 cactacgctg atttataaga tctaccgcga aggctttacc ggactggatc tggcttcgtc
    38161 tgccgcgcag tcggtggtgt tgatgttcct cgtcatcgtg ctgacggtgg tgcagttccg
    38221 ctatgttgaa agcaaggtgc gttaccaatg attgagaacc gtccgtggct gacgatattc
    38281 agccatacca tgctgatcct cgggatcgcg gtgatcctct tcccgctgta cgtggcgttt
    38341 gtcgcggcga cgctggataa acaggccgtc tatgccgcgc cgatgacgct catccccggc
    38401 acacatctgc tggaaaatat ccacaacatc tgggttaacg gggtaggcac gaatagcgcg
    38461 ccgttctggc ggatgttgct taacagcttt gtgatggcgt tcagcattac gctcggcaaa
    38521 attaccgtct cgatgctctc ggcatttgcc attgtctggt ttcgttttcc gctacgtaac
    38581 ctcttcttct ggatgatttt tatcaccctg atgctgccgg ttgaagtacg tatcttcccg
    38641 acggtggaag tcatcgccaa cctgaaaatg ctcgacagct acgccggttt aacgctgccg
    38701 ctgatggcct cggcgaccgc cacgtttctg ttccgccagt tctttatgac cctaccggat
    38761 gaattggtgg aagccgcgcg gatcgacggc gcgtcaccaa tgcgtttctt ttgcgacatc
    38821 gtttttccgt tgtccaaaac taatctggcg gcgctgtttg tgatcacctt tatctacggc
    38881 tggaatcagt atttgtggcc gttgttgatt attaccgacg ttgatctcgg caccaccgtg
    38941 gcagggatca aagggatgat cgctacaggc gaaggcacca cggaatggaa ctcggtaatg
    39001 gcggcgatgt tgttaacgct tatccctccg gtggtgattg ttttagtgat gcagcgtgcc
    39061 ttcgtgcgcg gcctggtcga tagtgagaaa taagatggca ggactgaaat tacaggcagt
    39121 aaccaaaagc tgggatggca aaacccaggt cattaaaccg ctgacccttg atgtggcgga
    39181 tggcgaattt atcgtgatgg tcgggccgtc tggctgcggg aaatcgacgc tgctgcgcat
    39241 ggttgccggg ctggagcggg tgacagaagg cgatatctgg atcaacgacc agcgcgtgac
    39301 cgaaatggag ccgaaagatc gcgggattgc gatggtgttc cagaattacg cgctttatcc
    39361 gcatatgagc gtagaggaga acatggcgtg ggggctgaaa attcgcggca tgggcaagca
    39421 gcaaattgcc gagcgcgtta aagaagcggc gcgcattctg gaactggacg gtctgcttaa
    39481 gcgtcgtccg cgtgagctat ccggcggtca gcgccagcgt gtggcgatgg gccgcgcgat
    39541 tgtgcgcgat ccggcggtgt tcttgtttga tgagccgctc tctaacctcg atgccaagct
    39601 gcgcgtgcag atgcgtcttg aactgcaaca gttgcaccgt cgcctgaaaa cgacttcact
    39661 ctacgttact cacgatcagg ttgaagcgat gacgctcgcc cagcgagtaa tggtgatgaa
    39721 cggtggcgtt gccgaacaga ttggcacacc agttgaagtc tacgaaaagc ccgccagcct
    39781 gtttgtggcg agttttatcg gcagcccggc gatgaacctg ctgacaggcc gcgtgaataa
    39841 tgaaggcacg catttcgaac tggacggcgg tatcgtgcta ccgctaaacg gtggctaccg
    39901 tcagtatgcc gggcgtaaaa tgactctcgg cattcgcccg gaacatatag cgctaagctc
    39961 gcaggcagaa ggtggtgtgc cgctggtgat ggacacgctg gagatcctcg gcgcagataa
    40021 cctggcgcac ggacgctggg gcgagcagaa gctggtggtg cgactggcgc atcaggagcg
    40081 cccgacggca ggcagcacgc tgtggctgca tttggcggaa aatcagctgc atctttttga
    40141 tggtgaaaca ggacaacgag tatgagtaac tggccttatc cccgcatcgt cgctcatcgt
    40201 ggcggcggta agctggcccc ggaaaacacc ctggcggcaa tcgacgtcgg ggcaaaatac
    40261 ggtcataaga tgatcgaatt tgacgcgaag ttatcgaaag atggcgagat cttcctgctc
    40321 catgacgaca atctcgaacg caccagcaac ggctggggcg tcgcgggtga actgaactgg
    40381 caggatttac tgcgcgtgga tgcgggcagt tggtacagca aagcgtttaa aggtgagccg
    40441 ctgccgttgc tttcgcaggt ggcggaacgc tgtcgcgaac acgggatgat ggcgaatatc
    40501 gaaatcaaac ccaccactgg cactggacca ttaacgggca aaatggtggc gctggcggca
    40561 cgccaactgt gggccggtat gacgccgccg ctgctgtcat cgtttgagat tgatgcttta
    40621 gaagctgcac aacaggcggc accggaactg ccgcgtggtt tattgcttga tgagtggcgc
    40681 gacgactggc gcgaactgac cgcgcggctg ggctgcgtct ctattcatct caatcataag
    40741 ttactcgata aagcgcgagt gatgcagttg aaagacgccg gattacggat tctggtttat
    40801 accgtcaata aaccccagcg cgcggcagag ttgctgcgct ggggcgtgga ttgcatctgt
    40861 accgatgcga ttgacgtgat tggtccgaac tttacggccc aatagttttc aacggaatgt
    40921 caggctgcgg cgtgctgggc tgattcagca tgtcgccgtt tctcctttcc ggcagcatat
    40981 gctgctgatt caacgaacta tccggattac ggttgctgtt taacatcccg ccgttggtgt
    41041 tgggcagcat ttgctgccgg gcgggatttc gctcccccgg ctgtgactgc aacacccgct
    41101 gagaattatt gtttatctgg ttttctaaat gctgctgttg caactgagtt tgcgttttca
    41161 gttgctgatt cagcatccct ttttgctgga tttgctgagt ctgcatctgg gtttgcatcc
    41221 gctgctggct gggtatctga taccccggct ggttagggtt gttcagagta ttaatgggct
    41281 gtgcaaagcc gacaaacggc aggagtgccg taagaatcag aagtcgtttc atcgcgtatc
    41341 ctcctctgaa gatatccttt aagtttactc gcttcccgac aaaacgatga ttaattcaga
    41401 gttatatacc aggcttagct ggggttgccc cttaatctct ggagaataac gatgataaaa
    41461 ccgacgtttt tacgccgggt ggccattgct gctctgctct caggaagttg ttttagcgcc
    41521 gccgccgcgc ctcctgcgcc gcccgtctcg tatggtgtgg aggaagatgt cttccacccg
    41581 gtacgcgcga aacagggaat ggtagcgtct gtggacgcca ctgccactca ggtgggggtg
    41641 gatatcctca aggagggcgg gaatgccgtt gatgccgccg tggcggtggg ctacgcgctg
    41701 gcggtaacgc atccgcaggc agggaatctg ggcggtggtg gttttatgtt aatccgctcg
    41761 aaaaatggca ataccacggc tatcgatttc cgcgaaatgg cacccgccaa agcgacccgc
    41821 gatatgttcc tcgatgatca gggcaacccg gacagcaaaa aatcactcac ttcgcatctg
    41881 gcttccggca caccgggtac ggtagcaggt ttctcgctgg cgctggataa atacggcacc
    41941 atgccgctga acaaagtcgt gcagcccgcg tttaaactgg cacgcgatgg ttttatcgtt
    42001 aacgacgcgc tggctgacga tctcaaaacc tacggtagcg aagtgttgcc gaatcacgaa
    42061 aacagtaaag ctatcttctg gaaagagggc gagccgctga aaaagggcga cacgctggtg
    42121 caggcgaacc tggcaaagag cctggagatg attgctgaaa acggcccgga cgaattctat
    42181 aaaggcacga ttgcggaaca gatcgcccag gagatgcaga aaaacggtgg cttgatcact
    42241 aaagaagatt tagcggccta taaagcggtc gaacgcactc cgataagcgg cgattatcgc
    42301 gggtatcagg tttactccat gccaccgcca tcctccggcg ggatccatat cgtacaaatc
    42361 ctcaatattc tggaaaactt cgatatgaag aaatacggct ttggcagcgc cgatgcgatg
    42421 caaatcatgg cagaagcgga gaaatacgcc tacgccgacc gctcggaata tcttggcgac
    42481 ccggattttg tcaaagtccc gtggcaggca ctgaccaata aagcctatgc caaatccatt
    42541 gccgatcaaa ttgatatcaa caaagcgaag ccgtccagtg agattcgccc tggcaagctt
    42601 gcgccttatg agagtaatca aactacccat tactctgtag tggataaaga cggtaacgcg
    42661 gtggcggtga cctatacgct gaacaccacc ttcggtatgg gcattgtcgc gggcgagagc
    42721 ggtattctgc ttaataatca gatggatgat ttctccgcca aaccgggcgt accgaacatt
    42781 tacgggctgg tgggcggtga tgccaacgcc gtcgggccga ataaacgtcc gctgtcatcg
    42841 atgtcaccaa ccattgtggt gaaagacggt aaaacttggc tggttaccgg tagtcctggc
    42901 gggagtcgga ttattaccac cgtgc
//
LOCUS       DAZPDU010000014        42304 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZPDU010000014 DAZPDU010000000
VERSION     DAZPDU010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42304
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.014"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..2885)
                     /locus_tag="ACHURS_000687"
     CDS             complement(<1..2885)
                     /locus_tag="ACHURS_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR1236498.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDASTSAREAATHAADAADSARAASTSAG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSAAATSAGAAKTSETNASASQQSA
                     ATSASTATTKASEAATSARDASASKEAAKSSETSAASSASSAASSATAAGNSAKAAKT
                     SETNAKSSETAAAQSASAAADSKTAAASSASAASTNAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQATAAARSASAAKTSETNAKASETSAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKGSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSETLAATPKAVKSAYDNAEKRLQKDQNGAD
                     IPDKGRFLNNINAVSKTDFADKRGMRYVRVNAPAGATSGKYYPVVVMRSAGSVSELAS
                     RVIITTATRTAGDPMNNCEFNGFVMPGGWTDRGRYAYGMFWQYQNNERAIHSIMMSNK
                     GDDLRSVFYVDGAAFPVFAFIEDGLSISAPGADLVVNDTTYKFGATNPATECIAADVI
                     LDFKSGRGFYESHSLIVNDNLSCKKLFATDEIVARGGNQIRMIGGEYGALWRNDGAKT
                     YLLLTNQGDVYGGWNTLRPFAIDNATGELVIGTKLSASLNGNALTATKLQTPRLVSGV
                     EFDGSKDITLTAAHVAAFARRATDTYADADGGVPWNAESGAYNVTRSGDSYILVNFYT
                     GVGSCRTLQMKAHYRNGGLFYRSSRDGYGFEEDWAEVYTSKNLPPESYPVGAPIPWPS
                     DTVPSGYALMQGQTFDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGR"
     gene            complement(2950..3549)
                     /locus_tag="ACHURS_000688"
     CDS             complement(2950..3549)
                     /locus_tag="ACHURS_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFR1236499.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHARTNVPG
                     SDDLNGINVKYRYEFTDTLGMVTSFSYAGDKNRQLTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKRHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIMGVGYKF"
     gene            complement(3616..7014)
                     /locus_tag="ACHURS_000689"
     CDS             complement(3616..7014)
                     /locus_tag="ACHURS_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFR1236500.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQSPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGSTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFMLRLTVAADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAANAWGQQGDPASVSFRIAAPAAPVTIELIPGYFQITAVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETTARYLGTALYWIAASSNIKPGYDYYFYIRSVNTVGKS
                     AFVEAVGRASDDAEGYLNFYKGLINKTHLGKELLENFELTEDNASRLEEFSKEWKDAN
                     DKWNAMWGVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETP
                     MFVAQGNQIFMNDVFLKRLTAPTITSGGNPPAFSLTPDGKLTAKNADISGSVNANSGT
                     LNNVTINENCQIKGKLSANQIEGDIVKTVSKSFPRTNSYASGTITVRISDDQKFDRQV
                     MIPPVLFRGGKHENFNSNNQQSYWYSTCRLRVTRNGQEIFNQSTTDAQGVFSSVIDMP
                     AGQGTLTLTFTVSSSGANNWTPTTSISDLLVVVMKKSTAGISIS"
     gene            complement(7075..7647)
                     /locus_tag="ACHURS_000690"
     CDS             complement(7075..7647)
                     /locus_tag="ACHURS_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFR1236501.1"
                     /translation="MKTGAEAIRAMATQLPAFRQKLSDGWYQVRIAGQDVSTSGLTAQ
                     LHEALPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGM
                     TGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNIYFSSLDNMVAQGNVMPVLY
                     GEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            complement(7644..8387)
                     /locus_tag="ACHURS_000691"
     CDS             complement(7644..8387)
                     /locus_tag="ACHURS_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR1236502.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGICNDLAAASTFV"
     gene            complement(8393..9091)
                     /locus_tag="ACHURS_000692"
     CDS             complement(8393..9091)
                     /locus_tag="ACHURS_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFR1236503.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLMVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            complement(9091..9420)
                     /locus_tag="ACHURS_000693"
     CDS             complement(9091..9420)
                     /locus_tag="ACHURS_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1236504.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEAMVLESFLEEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            complement(9417..11978)
                     /locus_tag="ACHURS_000694"
     CDS             complement(9417..11978)
                     /locus_tag="ACHURS_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR1236505.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKKRLERDAAQKRVDQQRQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVYAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQEHLNALAQQADKFA
                     QQQRAKRAAIDAKNRGLTDRQAAREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGSWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRAEG
                     INFNQNNHVVIQNDGTNGLPGPQMMKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            complement(11971..12405)
                     /locus_tag="ACHURS_000695"
     CDS             complement(11971..12405)
                     /locus_tag="ACHURS_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFR1236506.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPTSPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            complement(12387..12809)
                     /locus_tag="ACHURS_000696"
     CDS             complement(12387..12809)
                     /locus_tag="ACHURS_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFR1236507.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPQKTKQPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGSAEPVSAGKCSTVS"
     gene            complement(12825..13565)
                     /locus_tag="ACHURS_000697"
     CDS             complement(12825..13565)
                     /locus_tag="ACHURS_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1236508.1"
                     /translation="MPVPNPAIPVKGAGTTLWVYNGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASTSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGEFAAVAEITVTAS"
     gene            complement(13573..13968)
                     /locus_tag="ACHURS_000698"
     CDS             complement(13573..13968)
                     /locus_tag="ACHURS_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFR1236509.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            complement(13965..14543)
                     /locus_tag="ACHURS_000699"
     CDS             complement(13965..14543)
                     /locus_tag="ACHURS_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1236510.1"
                     /translation="MVIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARIVLPRRRRRKKGQ
                     RSALKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            complement(14555..14908)
                     /locus_tag="ACHURS_000700"
     CDS             complement(14555..14908)
                     /locus_tag="ACHURS_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFR1236511.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDEVRQLRRGDTLTIGEENFWIDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            complement(14920..15315)
                     /locus_tag="ACHURS_000701"
     CDS             complement(14920..15315)
                     /locus_tag="ACHURS_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFR1236512.1"
                     /translation="MTKDELIARLQVLGEQLNRDVSLTGTKEELALRVAELEEELDGT
                     DDAAGQDTSVSPENALTGHENEVVSAQPDTVTDTADLVTVVALVTLHTDALHATRDEA
                     VAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            complement(15357..16382)
                     /locus_tag="ACHURS_000702"
     CDS             complement(15357..16382)
                     /locus_tag="ACHURS_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFR1236513.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIILQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSVAN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSNSELETAVKDLGEAVSYKGMYGDTAIVVYSGQYVENDVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDA
                     FVSVQLA"
     gene            complement(16438..16770)
                     /locus_tag="ACHURS_000703"
     CDS             complement(16438..16770)
                     /locus_tag="ACHURS_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFR1236514.1"
                     /translation="MTSKEIFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTAT
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            complement(16780..18099)
                     /locus_tag="ACHURS_000704"
     CDS             complement(16780..18099)
                     /locus_tag="ACHURS_000704"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFR1236515.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPATLALSVNDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVQAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            complement(18080..19681)
                     /locus_tag="ACHURS_000705"
     CDS             complement(18080..19681)
                     /locus_tag="ACHURS_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR1236516.1"
                     /translation="MKTSTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPSES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSKEQRDRLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIARRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            complement(19678..19884)
                     /locus_tag="ACHURS_000706"
     CDS             complement(19678..19884)
                     /locus_tag="ACHURS_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFR1236517.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            complement(19881..21806)
                     /locus_tag="ACHURS_000707"
     CDS             complement(19881..21806)
                     /locus_tag="ACHURS_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFR1236518.1"
                     /translation="MNISNSQVDRLRHFVRAGLRALFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDDDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPASVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSISRICWDIGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDSAAT
                     NKKTLADYARALSGEDE"
     gene            complement(21781..22326)
                     /locus_tag="ACHURS_000708"
     CDS             complement(21781..22326)
                     /locus_tag="ACHURS_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFR1236519.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIKWYAERDAEIENEKLRREVEELRLASEADLHPGTLEFERHRLTRAQATAQELKNA
                     KESAEVVETAFCTFVLSRIAREISSILDGIPLSVQRRFPELDNRHIDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            complement(22466..22690)
                     /locus_tag="ACHURS_000709"
     CDS             complement(22466..22690)
                     /locus_tag="ACHURS_000709"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1236520.1"
                     /translation="MIRGFVPHARQLCAGVEETGHVFRSVRPWSHDAGLSDIEFIANK
                     PERLRVLSGDPAGYGAEGARVFAIYENFPV"
     gene            complement(22924..23373)
                     /locus_tag="ACHURS_000710"
     CDS             complement(22924..23373)
                     /locus_tag="ACHURS_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1236521.1"
                     /translation="MQEEAKKILVDLLNNASDGIDSAIAFSQAQIPDVVHQLLVWNIV
                     DSLIKTLIAISTIPLVIWFMKKQCKKVEIGKFDNDGRSCDNGQPKYKPTMIWESDGRL
                     SAFVLPLVAVFILWLSFIISIVANMTWLKIWLAPKLYLIEYAASLIK"
     gene            complement(23499..24359)
                     /locus_tag="ACHURS_000711"
     CDS             complement(23499..24359)
                     /locus_tag="ACHURS_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1236522.1"
                     /translation="MKELQKINSIMERLIALELKLADCGKLCAEKQNELYREFVTIVS
                     ELMPIVNNCLMTSRFFNVDATTSRKFTVKIQERVSGSVTREMAEQFVAMSNKTKRKKA
                     LLVTVNQLSGRVDFQVRQGGRVLVNDAFVGRCFSPYSMKYDVDADDQDISISWKSETA
                     GIVLTAELLSDDKNDGQHHLDEKNHISEICMKPRSGMTLLSRTLAAGFSATEKRVLLV
                     VPEDADIRPLNGCDVISAKTLHTRIMPVPDVVIIDDIEKCKSVSSTATIKRILRCSTS
                     AVVFQKQLHN"
     gene            complement(24640..24951)
                     /locus_tag="ACHURS_000712"
     CDS             complement(24640..24951)
                     /locus_tag="ACHURS_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFR1236523.1"
                     /translation="MKIYIAGPMTGYENFNRDAFNKEADRLSRHGHSVLNPATLPNGL
                     TQREYMDICFAMLRCADAILMLPGWKASAGATAEYHYAYKMEMPVFTTLNYPPACSSA
                     V"
     gene            complement(24993..25460)
                     /locus_tag="ACHURS_000713"
     CDS             complement(24993..25460)
                     /locus_tag="ACHURS_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR1236524.1"
                     /translation="MSRVTAIISALVIGIIVCLSWAVNHYRDNAIAYKEQRDKNAREL
                     KQATATITDMQQRQRAADALDAKYTKELANAKAENDALRRKLDNGGRVLVKGKCPVPA
                     SAKTSSASGMGNDATVELSSVAGRNVLGIRDGIIRDQTALRTLQEYINTQCLN"
     gene            complement(25457..25954)
                     /gene="rrrD"
                     /locus_tag="ACHURS_000714"
     CDS             complement(25457..25954)
                     /gene="rrrD"
                     /locus_tag="ACHURS_000714"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFR1236525.1"
                     /translation="MQSSLRKAVTAAIGGGAIAIASMLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCYGHTGKDIMLGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWIYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            complement(25954..26226)
                     /locus_tag="ACHURS_000715"
     CDS             complement(25954..26226)
                     /locus_tag="ACHURS_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1236526.1"
                     /translation="MKMDERYSSASYGSAGLAAFFASLSLQDWGFIIGVAFSIILGVL
                     TYRLNKREQMKRTKILQDILDKTNTNNLSATAMVIGELGKRAPKEI"
     gene            complement(26322..27398)
                     /locus_tag="ACHURS_000716"
     CDS             complement(26322..27398)
                     /locus_tag="ACHURS_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFR1236527.1"
                     /translation="MFKTTNIHGAQLVCADSLQFIKTIPDNSVNLIATDPPYFGVKAN
                     AWDNQWDSDADFLGWIDEFLAEFWRILAPNGSLYMFTGSRRASKIELLTRDRFNVLNH
                     IIWAKPSGMWRGCHKESLRAYFPATERILFAEHYGASGYAKGQSGYASKCADLRKNIF
                     SPLIDAFSLARRQLDISAADINSATGKQMCSHWFSYSQWRLPSLIDFNKLCELFRRKA
                     DSLGVPCPYPFNVDYSEHEKRYSDLKLHYEEVKKQYDDLKAQYESLRRPFHVTADVPY
                     TDVWEFPPVQYYPGKHPCEKPAAMMEHIIKSSSRPGDIVADFFMGSGSTIKAALKLGR
                     QAIGIEIEGERYLQTVDEVKKLFE"
     gene            complement(27666..27893)
                     /locus_tag="ACHURS_000717"
     CDS             complement(27666..27893)
                     /locus_tag="ACHURS_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1236528.1"
                     /translation="MNINEIRGNMTEAALSVECVMRGYPRISLKELSEACFLSQAAVE
                     FIIEQMICFGVAKRSGSGRYSLTDEYKQATF"
     gene            complement(27907..28128)
                     /locus_tag="ACHURS_000718"
     CDS             complement(27907..28128)
                     /locus_tag="ACHURS_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001543371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1236529.1"
                     /translation="MNYQGNEKMRGDVAEIANELYELWQKVERFEKEYGFNSTNLTDR
                     LAGRLIGTMGPKLAELNRFMADVDFQFQD"
     gene            28292..28603
                     /locus_tag="ACHURS_000719"
     CDS             28292..28603
                     /locus_tag="ACHURS_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR1236530.1"
                     /translation="MFNVITHPAALDELLELPDDLRGRMTRLIERLEKEGNKLKMPHS
                     RVIGGGLFELRVGDKNIARTLYAYATGNEIYLLHAFVKKTQKTPVKAIEIARTRLKEM
                     N"
     gene            28603..28890
                     /locus_tag="ACHURS_000720"
     CDS             28603..28890
                     /locus_tag="ACHURS_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1236531.1"
                     /translation="MKAKGIPFNEVKEKLLNTPEAIKGYEEADKELEMVEMLYEMRER
                     AGLTKSALAERMGLRPSAISRLESNPLGASMKTLSKYAQACGATININAVY"
     gene            complement(28934..29137)
                     /locus_tag="ACHURS_000721"
     CDS             complement(28934..29137)
                     /locus_tag="ACHURS_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1236532.1"
                     /translation="MTEASVSTLEELGSNLKDIDAVLDLVSVALASPEASLHLGEISR
                     LVSMSREIAQNCQQAIAVECLHH"
     gene            complement(29394..29720)
                     /locus_tag="ACHURS_000722"
     CDS             complement(29394..29720)
                     /locus_tag="ACHURS_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1236533.1"
                     /translation="MALTAAERQRRRRERLKKEGTSRRDWILEPDELRMLGEICVLRR
                     PGRVPYSENEVIGLLIRKNYKELQKQLSTTCPRCGQKMPVSECIFDGEGSCHLTTTRL
                     KLVLKV"
     gene            complement(29736..30410)
                     /locus_tag="ACHURS_000723"
     CDS             complement(29736..30410)
                     /locus_tag="ACHURS_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFR1236534.1"
                     /translation="MKFSKFSELVNRILLNRILSNNHSDRRDMDVTIVVHSPGSIGST
                     PSVEVQSIHAGFDWDSGKVLIFPAQPLTTLTPEQITDITDSVRKGQSWHAYQEYKKHK
                     EQLEKLSIELESAKNHIAELEAKPVSQTYKLTFEQWLEQQHDKIDVDCGCVSTEMLMH
                     WIRVAYEAGNSPVTPDGHIQFSVSLPAACGGDKYFIDGVFQPLRYERDCEKAVVAAGG
                     VVNWVK"
     gene            complement(30407..30697)
                     /locus_tag="ACHURS_000724"
     CDS             complement(30407..30697)
                     /locus_tag="ACHURS_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 family protein"
                     /protein_id="HFR1236535.1"
                     /translation="MNIDTTITIDTALNTGLALLGWFYIMFSAGKWAASVFLKQWGKR
                     RKQEKRQKALEAFYDAFELSRIEPGTTAKIATKGDLMIVMFRQERAEKGESA"
     gene            complement(30697..31116)
                     /locus_tag="ACHURS_000725"
     CDS             complement(30697..31116)
                     /locus_tag="ACHURS_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR1236536.1"
                     /translation="MAKAFTPEQREELNKQIVELVRLNGRGTVRQLADETGISRCAVS
                     RLSRELAASGDLYISGSGIFLSAQARKDWQNARKKLSRVKPKKSVVVDPDLIWSLPDG
                     EIRRYDRRLNMICHDCRKSEVMQRVLAFYQGNFQEVA"
     gene            complement(31116..31673)
                     /locus_tag="ACHURS_000726"
     CDS             complement(31116..31673)
                     /locus_tag="ACHURS_000726"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFR1236537.1"
                     /translation="MIDLETMGTNTCAPIVSIGAVFFSPESEELGPTFYVPVNLRSSM
                     LNGGIADGDTILWWLKQSKEARAAICTNDALDIKDALFELSHFITCHACNLKKLKVWG
                     NGATFDNVILRGAYERVGLACPWEYFNDQDVRTIVNLGQFIGFNPKKDMPFDGERHNA
                     LADAIHQAKYVSAIFSRLVKGQGES"
     gene            complement(31739..31912)
                     /locus_tag="ACHURS_000727"
     CDS             complement(31739..31912)
                     /locus_tag="ACHURS_000727"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1236538.1"
                     /translation="MTHDEAERLSDTYRRRGKKVLVVRSDFLGDGYCVYVHLPESERT
                     PKPSRTYQQKIWV"
     gene            complement(32176..32886)
                     /locus_tag="ACHURS_000728"
     CDS             complement(32176..32886)
                     /locus_tag="ACHURS_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFR1236539.1"
                     /translation="MELEYIRSCVSTALADVHYRQRGILEVQLEQMRLGRSGRFNNKP
                     VRSISVGDDNSYEVSVPAEPVRFHQGKTFKQSSMLLTDIDFQSASWRRAIGQLNNEES
                     AWLYYCYGCKPDYNNDVIVCQWLWLDFLVAHSGGGFKKMKASTKKAMRKLIYYAAQQV
                     KSELTCAEAVDEREQDRHLSFLLNISIDSWRQDYKERWLLIKSRCLSLNRTALLNAAE
                     KRSEIIKRHRAGSAILPL"
     gene            complement(32876..33097)
                     /locus_tag="ACHURS_000729"
     CDS             complement(32876..33097)
                     /locus_tag="ACHURS_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFR1236540.1"
                     /translation="MKPEELIRHFGSVEKAAAGVGVTPGAVYQWLTAGEIPSLRQSDI
                     EVRTAYKLKSDFTTRRVKQSIKESRKRGA"
     gene            33189..33785
                     /locus_tag="ACHURS_000730"
     CDS             33189..33785
                     /locus_tag="ACHURS_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001495876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1236541.1"
                     /translation="MKTRGERLKARRLEMKLTLREVAEAVDISIAGVQNLERGDVMPS
                     LEIGIALAKCLRKPVHWVLYGTEYDPDRIPVIGTTESGPDEEWKPGQVPNTERFLPFI
                     SQRNTVYALTISNLTQGNYQPGDFLLVDSALELVPGEDVLVRDTAGNITIQRLARFDD
                     ENYYLDGTNSQRAIFRKSDLEFVHQIVGTIKSFMVEGR"
     gene            34031..38026
                     /locus_tag="ACHURS_000731"
     CDS             34031..38026
                     /locus_tag="ACHURS_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication protein, CyRepA1 family"
                     /protein_id="HFR1236542.1"
                     /translation="MTLNDFYADRFGSDPFSLIEAARDELTELAQMAGINWPACSDNI
                     QLNPRGGVERYSSYNKTSPEALEKSLKGRVEIYSRLEHSKGIDYPFINFVHKGSDAGS
                     WSGFSFLFSEYRREQQRNNATVVAQPEEERERIERQAEARRRREEQKRINDLKNNQMD
                     HERLLGWLAFHSAWEHAQAEDGSWPYAIKKGIRDVFSSCDVRRVTSHDSAKWSRGPTT
                     YMAIPLAHLDGRYDGRIVGWQRIDKQGGKYQTSAVTSGDFVGACFVIGELKGAQNIAV
                     TEGFATGASIWLATKKDKRFDAVVVAVSANNMVHVVEQLVNMYPAAKITCALDNDRKS
                     ATEGKGNTGLRTGYEILSKFNDVRCVYPTFEDDPQMECSDFNDLHKLRGLRETARQLF
                     ATANRLKTGTDLLSLTLNRLKTIKRDNRRTFAKELLRAVDIGMLTCPVPNSPSDLFNM
                     FCIVLREMGLENIYRATVKDHIARRLNKKCRIAQAPRSFSERITDPNKRPQHITYKRF
                     ETSVMTDDVLKYVQSLQGIVIVRAGMGSGKSTGLLRPLMHSSERGISVAHRVSLIGGL
                     WEMMTEGKGVKADILHYQDPGYQEMAPYASKLTICINSIVKNCWQPLMRQHDYFGFDE
                     ATQGLRAVLSGRAMENPVAVFNTLIDALARTEQHPIMVDADANDLLVDLAELAMKRRE
                     ELGLPAWLQIHVIELPVDVRNRETGEPIRVFYTEKDRIMTEVVKAVSDGEKIMLATDS
                     STFAEDVTATLRMHFPGKKFLCVNQKNKPEPEVEAFTNQPKKMVKKYDGLIYSPSISS
                     GVSIEQKHFDRHFGMFCGEVVPSDAVQMLRRDRTAKEFIIGFDKARAKRETDPQKIER
                     AFAQALLATAGMNGELTDVVFDGDRISMGVANTDFTRMKIKAAAIEATARNDYASNMI
                     CIMHSDGYKVSPLATDYAANDIGKDLRKEARDIVWEQTLDLHLNTETPQESEREELLK
                     KRALTLEEQAKLVRWDIENELKLPVEEGTLKFYFDGARDKVRRYETMLLDEVTARRYD
                     REEAAINFTYAYRQAGQWQYFVVTAMTREQADDVFKTKFPGIIDHKVKATPTVEVGAR
                     GFYALKSATLRQYFIDCGINPETMTGEATQERLKFARDNLMTAERRDLLNNVLHIGGF
                     MTPKGKPKVPEALFKNICDSLGLKTGKRRGRDGDKRPTLRFVEPESAAFMLDIIVQRQ
                     KDGLTLKTRKAEKTAIEMDRDLDLNIYMDDKTRSTNEQVQNAPHSVISEALAVLPVAV
                     PESWAMSALSSEELATMSSWSSASIAMTFASLYLTEFMDHLSSNELRRLREYITGNSS
                     CSYEEQGAFYG"
     gene            38215..38541
                     /locus_tag="ACHURS_000732"
     CDS             38215..38541
                     /locus_tag="ACHURS_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1236543.1"
                     /translation="MSTTAFLDKLDYRQLQFCRDECERRIRAIQEEEKKVAWAVTDRG
                     LIFGWFRTEDYLKAVELLARKAAERWERADKENPETKYELNLYIEGNRLPLSEYNALF
                     ADGEWG"
     gene            38544..38939
                     /locus_tag="ACHURS_000733"
     CDS             38544..38939
                     /locus_tag="ACHURS_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1236544.1"
                     /translation="MAVQSYKYESPLDKGYIRIKLSRYQHKAIFKVRKIRILDAYAYY
                     YNGENVVVEHFLARWFVALIFVPLLLIGTFVDGFPSTWREIKKGLFPRKYGRFSQDSW
                     RVIPGKHSVDEQPIVDFWLNRMADAKKVD"
     gene            38967..39254
                     /locus_tag="ACHURS_000734"
     CDS             38967..39254
                     /locus_tag="ACHURS_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1236545.1"
                     /translation="MKSFIFVLCFAFSAAVYAQEDSISVINSSADLACKNNPDAASKE
                     SCKELLLSTAAFASENAKFYSAFCRGYVKDEDKEKCDDAGKLVRYFQSLNQ"
     gene            39631..39795
                     /locus_tag="ACHURS_000735"
     CDS             39631..39795
                     /locus_tag="ACHURS_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFR1236546.1"
                     /translation="MFKFKFAAIVRTDKKSHIHHLSTIASSEREARRQFASRFVLVLS
                     ARIPVREVAA"
     gene            39792..40025
                     /locus_tag="ACHURS_000736"
     CDS             39792..40025
                     /locus_tag="ACHURS_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1236547.1"
                     /translation="MNQVKMNTVGLLESLDERLAQVYALVSSAHRTISSCEASIYLSE
                     AGKLLQIACELTHEAQGYSASLSSQLLHDKEAE"
     gene            40022..40186
                     /locus_tag="ACHURS_000737"
     CDS             40022..40186
                     /locus_tag="ACHURS_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HFR1236548.1"
                     /translation="MSALAVFSFQEEHQVRVVMINGEPWFVASDVCMAAGIDSTAIRK
                     LDEDEKGQAR"
     gene            complement(40232..42127)
                     /locus_tag="ACHURS_000738"
     CDS             complement(40232..42127)
                     /locus_tag="ACHURS_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="telomere resolvase"
                     /protein_id="HFR1236549.1"
                     /translation="MSKVKIGELINSLVSDVESIDNSDRPQGEKTKKIKAVALKFKNA
                     LFNDKRKFRGKGLEKRITANTFNAYMSRARKRFDDKLHHSFEKNIHRLSEKYPLYNEE
                     LLSWLSLPAADIRQRMSALQAKLKLILPLSEDLSNIRIGDNKSHNKLLKLSNKYKEWQ
                     FAISDLTSDNWKDKRDYLYKLLQQGSALLEELNQLKVNHEILYHLQLSPAERASIQQR
                     WADVLHNKKRNVVVIDYPKYMQAIYDIINSPATLNNLNTRSGMAPLAFALAALSGRRM
                     IEIMYQGTFTVSGKYTVDFLGQAKKRTSDDITRKIYTLCEAKTFVYLINVLRSCPAAS
                     DFDDVVKGYGDNDTRSENGRINAILAKAFNPWVKSFFKDDRRVYKDSRAIYARIAYEM
                     FFRVDPRWKNVDEDVFFMEILGHDDENTQLHYKQFKLANFSKTWRPNTGNENTRLEAL
                     QQLDDEMPGFARGDAGVRLHNTVKQLVEHDPSIKITNSTLRAFKFSPTMISRYLEFAA
                     DALGQFVGENGQWQLKIDSPEIVMPDEEELEAIPEQTDELSEDDDLDDDEIEIEEEEE
                     EESVEEQQPPAPSSPVSKPVFKPVRNIGDGSYVVEFHYNGQNYAWSGSANSPISAMQL
                     AWLTYFG"
BASE COUNT        10151 a        11546 c         9880 g        10727 t
ORIGIN      
        1 cgaccactgg caggttttcc cttaatcgtc cagccacgca tatcagggat cacgcctgac
       61 ggataagcgg ctgcaagttt cgggtaagca gatttgtcaa aagtctgccc ctgcatcagg
      121 gcataaccag acggaacggt atctgatggc cacgggattg gtgcgccgac tgggtagctt
      181 tctggtggaa gatttttcga ggtataaact tctgcccagt cttcctcaaa accataaccg
      241 tctcttgaag aacggtagaa cagaccacca tttctgtaat gcgccttcat ctgcaaggtc
      301 cggcaacttc cgactccggt atagaagtta accagaatat agctgtcgcc agagcgggtg
      361 acattgtaag cgcctgattc ggcattccag ggaacgccac catccgcatc ggcatatgta
      421 tccgttgccc ttctggcaaa agcagccaca tgcgcggcgg ttaaagtaat atctttggaa
      481 ccatcaaact caacaccaga aaccagtctt ggcgtttgca gctttgttgc tgttaatgca
      541 ttaccgttca gacttgcgga cagtttggtt ccaataacca gttcgccggt tgcgttatca
      601 atagcaaacg gtcttaatgt attccagcca ccataaacat caccttgatt ggtaagcagc
      661 aggtaagttt tagcgccatc attacgccat aatgcaccat actccccacc tatcattcga
      721 atctgattac caccacgcgc tacaatttcg tctgtggcaa aaagtttttt gcacgacaag
      781 ttatcgttaa cgattaacga atgagactca taaaaaccac gcccactctt aaaatcaagg
      841 ataacgtccg ccgcgataca ttcagtcgct ggatttgttg ccccaaactt ataggtcgta
      901 tcattaacaa cgagatcagc accaggtgcg gatattgaca ggccatcttc gataaacgca
      961 aaaacaggga aagcagcgcc atcaacatag aacacagagc gcaaatcatc gcccttatta
     1021 ctcatcatta ttgagtggat ggctcgttca ttgttttgat attgccagaa cattccataa
     1081 gcataacgcc ccctgtcagt ccagccacca ggcataacaa atccgttaaa ctcgcagtta
     1141 ttcatcggat cgcctgcggt tcgcgttgcc gtggtgataa tgacccttga tgccagttcg
     1201 cttactgagc cagcagaacg cataacaaca acagggtaat attttccaga tgttgcacct
     1261 gcaggagcgt taacccgcac ataacgcata ccacgcttat cagcaaagtc tgttttactg
     1321 accgcgttaa tgttgttcag gaagcgtccc ttatcgggta tatcagcgcc gttctggtct
     1381 ttctgcagac gtttctctgc attgtcatag gctgatttta ctgcctttgg cgttgccgcc
     1441 agcgtttcag acgtactgtt ggtcgcactg ctgagctgta ctatcccctt tttcgtcgta
     1501 cttgcatcct caagcgccac ggcggatgca atatcctctg cccgtttagc tgctgtctcg
     1561 gcgcgcgttg ccgcggattc cgccgtactt ttgctctgag ctgccgccgt cgcactgcca
     1621 gctgcctctg tcgccttcgt ggatgctgtc gtggcgctgc ccttcgctgc tgacgcctgt
     1681 ctggtcgcct catcttttga agcagacgca gatgatgccg atgacgccgc cgaactggct
     1741 gacgatgcgg ctgccgtttt tgaggattct gcgctggttt ccgacgcttt cgcgttcgtt
     1801 tcggatgtct tcgctgcgga agctgacctc gctgctgccg tggcctgttc agtggcttcg
     1861 ccagccttcg ttgtggctgt tgaagcggat gatgcggcgc tttctgccga ctttccggca
     1921 gcggtggcac tggctgaggc ctgcccggca tttgttgacg cggcgctggc agatgaggca
     1981 gccgctgttt ttgagtctgc tgctgcggag gcactctgtg ctgctgccgt ctcagaagac
     2041 ttagcgttcg tctcggacgt ttttgccgcc ttcgcggaat tgcctgccgc tgttgccgag
     2101 gaggctgcac tactggcgct cgaggctgcg ctcgtttctg atgattttgc cgcctctttt
     2161 gaagccgacg catctctggc tgaggtggca gcttctgacg ctttcgtggt cgcggtggat
     2221 gcagaagtgg cggctgattg ttgtgacgct gaagcattcg tttctgacgt tttcgccgca
     2281 ccggcactgg tagccgccgc gctttttgag gactctgcag cggcagcact ttttgatgct
     2341 tcagtggcct ttgttgatgc cgttcctgcg ctggaagacg ctgactgagc cgacgacgcg
     2401 gcctgtccgg ctgacgtgct ggctgcgcgt gctgagtccg cagcatcagc cgcatgggtt
     2461 gccgcctcac gggctgatgt gctggcatca ctggctgact tcttcgcggc tgccgtgttc
     2521 tgtgccaccg cggacgcgtt acgcgccacc tcttccacca tcagttcaaa acggcgcagt
     2581 gcctccggac gggcatcatc ctccgtcatg gcaccgagaa aatcattcag cgtaccgggt
     2641 tgggaatctt catacacggt gatggtcccg gcatgtgacg gcgggaatcc ttccaccaac
     2701 agaataacgc tgtactgacc gtactcaacg tccatgctgt aacgcccggc ttcatccgga
     2761 ttttctgagg ccagcgtgtt caccaccacc gtggtgctgt tacgttttgc tttcagctgg
     2821 attgtgcagt tctgtaccgg ttttcctgtg ccatctttca gtacacctga aatctttact
     2881 gccatattca ccccacaaaa aagcccgcct gaaccggcgg gctgtcataa cactgtgtta
     2941 cctggctaat cagaacttat aaccgacacc catgatgaaa ccgtcagtgc gccagtcgcc
     3001 actgccggag ccttcataag caatatcaat ggccacggat tcggtcgggt taaactgcac
     3061 gccagctccc cacgccagag acgtgttgct gtggcgaccg tcatcacttc cggtcagcac
     3121 gtcgtgcctt tttcccttgt tgtcagttac gcggagataa tccccggaaa aagtcgacac
     3181 acggctgtaa gccacgcctg ccatcgcata cgcgctgaac cattcattca cgcgtacaga
     3241 cggccccgcc atcacgctga accagcggtt acgcacggaa tcttcatgcc agcgggtatc
     3301 gctgtaatgg gtgagctggc gattcttgtc tcctgcatag ctgaacgacg tcaccatccc
     3361 cagtgtgtcc gtaaactcat aacggtattt cacgttaatc ccgttcagat catcactgcc
     3421 gggaacgttc gtccgggcat gaagataccc cgcgctcagc gtggactgat gttcagacgc
     3481 ccatgcaggc gcaccggata cggccagaca aatggctgcg gacaaaatgg cggcataaag
     3541 tttacgcata attacctctc gcttttctgc aataaaaaag gcgtcatttc tgacgcccgt
     3601 tctgggttat aaaattcagc tgatactgat acctgctgtg gattttttca tcaccacaac
     3661 cagcagatcg ctgatactgg ttgttggtgt ccagttattc gctcctgatg aagatacggt
     3721 gaatgtcagt gtcagcgtcc cctgtccggc aggcatatct ataactgagg aaaatacgcc
     3781 ctgagcatcc gtcgtggact gattaaaaat ctcctgacca ttgcgggtca ctcttaaccg
     3841 gcaggttgaa taccagtatg actgttggtt attactgttg aaattctcat gcttaccacc
     3901 gcggaataac actggcggta tcatgacctg ccggtcaaat ttctgatcat cactgattct
     3961 taccgtgatg gtgccactgg cataactgtt cgtgcggggg aaagacttgc tgaccgtttt
     4021 gacaatatca ccttcaatct ggttggctga cagtttcccc ttaatctgac agttctcatt
     4081 aatcgtgacg ttgttgagcg tcccggagtt cgcattcaca ctgccactga tatccgcatt
     4141 tttagcggtc agctttccgt ccggtgtcag ggaaaaggcc ggaggattgc cgccgctggt
     4201 aatggtgggg gccgtcaggc gcttcaggaa cacgtcgttc atgaatatct ggttgccctg
     4261 cgccacaaac atcggcgttt cattcccgtt tgccgggtca ataaacgcga tacggttagc
     4321 ggcaaccaga aactggctca gtttgccttc ctccgtgtcc tccatgctga ggccaatacc
     4381 cgcgacataa tgtttgccgt ctttggtctg ctcaattttg acgccccaca tggcattcca
     4441 cttatcgttg gcgtccttcc actctttcga aaactcctcc agtctgctgg cgttatcctc
     4501 cgtcagctca aagttttcca gcagttcctt tccgagatgc gttttattga tcaacccttt
     4561 ataaaaattc agataacctt ccgcatcatc gctcgcccga ccgacggcct ccacaaatgc
     4621 cgatttacca acggtattca cgctgcggat ataaaagtaa taatcataac ccggcttaat
     4681 attgctactg gcagctatcc agtacagcgc cgtgccaaga tagcgggctg tggtttcaac
     4741 ctgcctgata tccgcaatcc gcttttccga gaaccagaac tcaaactgca ccgtcgggtc
     4801 atatacagcc agtttcggga ccgctgttat ctgaaaatac cctggtatca gttcaatagt
     4861 gacaggcgct gccggtgcgg caatccggaa cgataccgat gcaggatcgc cctgctgccc
     4921 ccacgcattt gccgcccgga ctgtcagcct gtagttcccc agcgccagtt gcgtgaagcg
     4981 gtatgtggtt tccgtcgtcc gggccgtgct gaccagccgc tcactgccgt catccgctgc
     5041 cacggtcagg cgaagcataa agctcacgcc cttcaccacc ttcggcgtgt cccagcgcgc
     5101 cagcacctga tattccccgc tgtctgcggt gacttcggca gtcaggtgct gcaccgcggg
     5161 cggcgtgacg ccattcaccg tgccgctctg gtcgccgtca aagtgcgccc cgttatccac
     5221 gatggcttct ttttcgggta catgctgcac ggcggtgatg gcatacgtgc cgtcgtcgtt
     5281 ctcacggata ctcacgcagc ggaacaggcg ctggcgcaac gtcggcaact tcagccccca
     5341 cacgctgtat tctgcaacgc cgtcaggaac acggctcact tttaccttca cgccgtcggt
     5401 gacggactgg acctccacgc tgaccggatt gccacttccg tcaaccaggc ttatcagcgt
     5461 ggtaccggag gatggcagcg tgatttcacg gtcgagcgtc agcgtccggg tctggctgtt
     5521 taccgccagc acgcgcccgc cgatgctgat accggcatag tcatcatcgc agatttcaat
     5581 gacatcgccc ggtacatggc gaagcccttc ggcacccacg ctgaagtcca cggtctgcgt
     5641 ttccagcagt tccgttttaa tcagccacag cccggcgcgg tgtgcctgcc cccgactggt
     5701 acagccaaag gcatccatct tcgtgacatt acgaccgtaa cgggcaatgg cctgcgtgtc
     5761 ctccacaagc tctgttgccg tttcccagcc gttatccggg tcaatccagt tcacctcaac
     5821 ggcattatgg cggtccttca gggcgctgaa actgtagcgg aacggcgcgc catcatccgg
     5881 catcaccaca ttactgcggt tataggtcca caccttatcc gacggtcggt cctgcacgaa
     5941 cgtcagcgtc tgcccgttcc ataccggcat acagcgcatc gccgagcaga aatcactgag
     6001 cacatcccac gccttgcgct gtgtggtcag ccaggcatta caggtgatgc gcggctccgt
     6061 gctgccaaag ccgtccggca ccgactggtc acagtactgg ccgatgacat acagcgccca
     6121 tttatccaca tctgccgcac caagacgttt ccccatgcca tagcgcggat gggtcagcat
     6181 atcccacaga caccaggcca tgttgttgct gtatgccggt tttaacgttc cgtcccagat
     6241 accgctgtat tgccgcgtct gcggattata gttcgacggc acctgcagaa tgcgcccgcg
     6301 aagatgataa ttacggctca cctgctggct gccgaactgc tccgaatcca cctgtacgcc
     6361 gaccagtgcc gtgttcgggt agcactgttt cacatcgatg atttcggtgt atgacgacca
     6421 gagcgttttg ttctgcagct ggtctgtggt gctgtccggc gtcattctgc gcatccggat
     6481 attaaacggg cgcggcggca ggttatccac caccaccgag gccagatact gcgaggtggt
     6541 tttgccctta atggtgatgt ctttttccgt cacccagcca ccgttacgct gtatctgaac
     6601 cagcaggcga acttcagacg gattcctgtc tccctttgag gtggtttcca ccagtgcctg
     6661 tacaccgaag gtaaaacgca gtcggtcaat gtttgccgac gtgatggtcc gggtgatcgg
     6721 cgtgtcatat ttcacttccg tacccagcac cgtctcggag ccggaggatt caaatccctc
     6781 cggcggtgac tgctcctgct cacctgcccg gaacaccacc gtgacgccgg agatgttggt
     6841 attcccctca ctgtccagca ccggcgtact gttcagcagc acgcttttta atccatccac
     6901 cggaccttca accggccctt cgctgatggc atcgatcaca ctcagcagct gcgtggactt
     6961 caggttgtcc ttcgcttcgc gcggggtatg ccccttactg ctgcctttac ccattcgtca
     7021 tgctccataa acgataaaac cgcccggagg cgatttcaca taaaacattt tgcatcagcg
     7081 accaatcacc acaacctgac caccgtcccc ttcgtctgcc gtgctgatct cctgagatac
     7141 cacacgtgac cccacgcgca tttcaccata cagaacaggc ataacattgc cctgggcaac
     7201 catgttatcc agtgaggaga aatagatgtt ctgcttaccg ttatccgttg tctgtgtgcg
     7261 gggagttctg gctttcggtg ccagcatctg agccacaccg ccgagcacca tactggcacc
     7321 gagagaaaac agaatgccgg tcataccacc ggcaccaatg gcagcccccc atgctgcaag
     7381 ggtggctccg gcggtaaaga atgatccggc aatggcggct gctcccagga caatctggaa
     7441 tacaccacct gacttggccc cggcgactct gggaacaata tgaatcacag cgccgtcagg
     7501 cagagcctca tgtaactgcg ccgttaatcc ggacgtgctg acatcctgcc cggcaatacg
     7561 cacctgatac cagccgtcgc tcagcttctg acgaaacgca gggagctgtg tggccatcgc
     7621 ccggatggct tcggcacccg ttttcacacg aaggtcgatg cggcggccaa atcgttgcaa
     7681 atccccgtaa aggcagatgc gtgccatgcc cggtgacgcc agagggagtg tgtgcgtcgc
     7741 tgccatttgt cggtatacct ctctcgtttg ctcagttgtt caggaatatg gtgcagcagc
     7801 tcgccgtcac cacagtaaat ggcggcatga ttcggcaccg atgaaccaaa acagcacagc
     7861 agcacatcgc ccggctgcgc cgctgacaac ggcacctgat acagccctgt ggcctccaga
     7921 ttatccagat agagattctg accgtgacgc caccagtcat cctcacgatg aaaatccggc
     7981 atctcaatcc ccgccagatg gtaagcatcc cggaacagcg tgtaacagtc cgtcaccccg
     8041 tgctcaaagc gccgcccggt aagatgtggc acacagcgga acttgtgaat cgccccccgg
     8101 cagaccagcc accacggcaa atcactctgc acctgcagcc gccggtcggc ctcactcagc
     8161 cagggcagac caccggggtg gctgtggacc agtgccacaa tctcaccctg catctctgcc
     8221 tgcagccagt cttccggcga catacggaaa tacgcctccg gctcaccgga gatattcacg
     8281 caggggaaat atctttcccc ctccggcgtt cttaccacga agccgcacga ctccgctgga
     8341 gcacatcgcc gggcgtgcgc cagaatcgct gattctgtct ctgtcatggg atttactgcg
     8401 aaagtttgtt aatggaaagg aagccgccaa agttgccgac gttattgcga aacttacagc
     8461 cactcaggca tttgctgcat ttatccttcg tgatgtcgga cgttggctgg tcatattcat
     8521 ccgcgaccgc cggaccgtga taaccgcact catcgccgcg ataggtccag gtgcaggtgt
     8581 tggccagcat gatacgtccc ggaaaaacag cgccatctgt ttccgtcggc gtggacagta
     8641 caaaagaggc actcaccgcg ctcagttcgc tgcactgctc aatgcgccag cggctgatca
     8701 cctcctgctc cggatcggcg tcactgtttc cgttgacgaa gttcaccgca tccagaaaac
     8761 gggcgtaaac cttacgccgg accaccgttc cgccgaccag actctgtaga tcttccgcca
     8821 tcccggtgac cataccgtac aggttagaaa ccatcagcgt ggggcgcgta ctggtgcctt
     8881 tgccattcag ttcaaaacca ctcccctgaa tgggatacgg ctgatactgt cgcccctgcc
     8941 aggtgaccgg ctcacctttt tcgttctgct cattacagaa aaaataacgt tctccaccga
     9001 cctctgtcag atcgatttcc cagagcacca cgctggccga ctgctccgca cgggtgcatt
     9061 cattcagtgt ttcctgccgg atatcctgca tcagttcacc acctgttcaa actctgcgct
     9121 gaactcaaca cgcagcatac tgacccgcga cgaccatttt gcgcaggtca cctttatctg
     9181 ccgccactca taaggcggcg tccacagaaa ggctttccag cccccgtgct cttccagaaa
     9241 cgactccagt accatggcct cctcacgggg gacagaaagc gtcacgctgt acgttttcag
     9301 gttggcattc agcccggcag gcgctcgctg ggaatagcca tcaccaaagc gcacctttct
     9361 tacagaaggg gccgaagcca catccatacc gggtttcact ttccagcgga aggttttcat
     9421 cgtccacctc cggagaacag gccaccatca cgcatctgtg tctgaatttc atcacgggca
     9481 cccttgcggg ccatgtcata cacggccttc atcatctgtg gacctggcag accattcgta
     9541 ccgtcgttct gaatcaccac gtgattgttc tgattaaaat taatgccttc agcccgccgc
     9601 atctgcgccg gacttccggc accgcccaca taaccacctt ccgcatagcc gcgcatcagc
     9661 cggtagagat tccccacgcc aatccggctg gttgcctcct tcgtgaagac aaattcacca
     9721 cggtgaacaa tccccgctgg ctcatatttg ccgccggttc ccgtaaatcc cccggttgca
     9781 aaatggaatt tcgccgcagc ggcctgaatg gctgtaccac ctgacgcgga tgcgccgcca
     9841 ccaacagccc cgccaatggc gctgccgata ctcccgacaa tccccaccat tgcctgctta
     9901 agcagaattt ctgtcatcat ggacagcacg gagcgggtga agctgcgcca gttctgttca
     9961 ctgccggtca gcatcgccgc catattctgt gcaataccat caaaggtctg cgtggctgca
    10021 cttttaacct gtgacatact gtccgtggcg ctctcttccc actcactcca gccggacttc
    10081 aggcctgcca tccagctccc gcgaagctgg tcttcagccg cccaggtctt tttctgctct
    10141 gacatgacgt tattcagcgc cagaggatta tcgccatact gttccttcag gcgctgttcc
    10201 gtggcttccc gcgctgcctg ccggtcagtc agcccccggt ttttcgcatc aatggctgcc
    10261 cgttttgccc gttgctgctg tgcgaattta tccgcctgct gcgccagcgc attcaggtgc
    10321 tcctgatacg taaccttgtc accaagtgca gccagctggc gtttgtactc cagcgtctcg
    10381 tctttatgcg ccagcaggga tttctcctgt gcggacagct ggcgacgttg tgccgcctcc
    10441 tccagtaccg cgaactgact ttctgccttc cacaaatccc ggcgctgctg gctgattttc
    10501 tcattcgctc cggcatgctt ctccagcgtc cggagttctg cctgaagcgt cagcagggcc
    10561 gcatgagcac tgtcttcctg acgatcgccc gcatacacct tcacgccgga ctgtttcggc
    10621 tttttcagcg tcgcttcata atcctttttc gccgccgcca tcagcgtgtt gtaatctgcc
    10681 tgcaggattt ttccgtcttt cagtgccttg ttcagttctt cctgacgggc ggtatatttc
    10741 tccagcggcg tctgcagccg ttcgtaagcc ttctgcgcct cttcggtata tttcagccgt
    10801 gacgcttcgg tatcgctctg ctgctgcgca tttttgtcct gttgacgctg ttgatctacc
    10861 ctcttttggg ctgcatcccg ctccaggcgt tttttttcac gatcatccca gtaacgcgcc
    10921 cgtgcttcat cgttaacgaa ataatcatcc ttgcgcagat tccagatgtc gtctgctttc
    10981 ttaaacgcag cctctgcctt aatcagcatc tcctgcgcgg tatcaggacg accaatatcc
    11041 agcaccgcat cccacatgga tttgaatgca cgtgcagtcc tgtctgccca ggtctccagc
    11101 gtgcccatgt tctctttcag gcggcgggtc tggtcatcaa accctttcgt tgcggcctcg
    11161 ttcgccgcct gcaatgcccc ggcttcatcg ccggaacgct gcaactgagc aacatacgca
    11221 atctgctccg ccgtcacgtt atggaactgg cgtgccatcg ccgtcagccc cgacgtcggg
    11281 tctgtggtca gcttcccgaa ggcttcagcg accttgtcca cctccacgcc ggatgcagag
    11341 gagaaacgcg ccacactctg gctgatggac gcaatctgag cctcaccgct tacccccgcc
    11401 ttaaccagtg cgctgagtga ctcgctggtc tggttaaacg tcagccctgc cgcctgcccg
    11461 gctctggaca ggaccagcat acgatctgcc gtcagtcccg cctgattgcc ggaaaggacc
    11521 agcgttttgt tgaaatcgga cagggttgag ttgccctgat accaggcata cgccagcgca
    11581 ccggtcgcca ccgccagcga ggtggccccc accatcggca gggtgatcgc accggcaagc
    11641 cccctgaaca tggggatcat cccgccgaag gagtccttaa cctgaccacc ctgttgcagc
    11701 aggatcagcc acggactttg cccgcctgca agctgcgtgg ccacgtcggt gaactgtgca
    11761 ggcagcatac gcatggcggc tttatactgc ccgacggaaa tccccgcttt ctgtgcagcc
    11821 agcgcctgtc ggctcagcga ctgttcaaca actgccgctg tttttttcgc atcactttcc
    11881 gtaccggaaa aatgacgcct gactctggcc atctgctcgt caaatctggc cgcatccaga
    11941 ctcaaatcaa cgaccagatc gcctaccggt tcagccatac cggactcctc ctgcgatccc
    12001 ttctgatact gtcatcagca ttacgtcatc ctccgtcatg tccgccacat ccggggaagt
    12061 agggataact tcattcccgt ccgggccaaa gcggacacct ccggcaagcc ctgccgcttt
    12121 ctgcatcagc acatcatctt caggctcttc gtcagcctca cgccggttca gcagactgaa
    12181 atccagcgga tgcatatccg gatcgctgaa aaacaggctg agcacggtgt acgtcagccc
    12241 ggaaaagtgc atatccagca gaacatcatg aaaataatgg gtactgtaaa agcggtgcca
    12301 gtcggcatac tccgtggatg acatcccggc aagcatggca cgccagtcgg gtcgccccat
    12361 ttcgcgcgcc agtttcaggg caaaactcag ctcaccgtcg aacactttcc cgcagaaaca
    12421 ggctctgcag acccggcgtc ctctgcctgt tcaggggcat cattcaccac aaactcatac
    12481 ataccggaca gccggtacac cacgttttca gcatgagaaa ttgcctccgt gggccaggtg
    12541 gtaagcactt cctgctcaat ctgtttaacg gcttcattca tggaaggctg ctttgtcttc
    12601 tgcggatggt tatgccacag ggacatcgcc accacaaaag caccggttct gatggcgtct
    12661 tccacagtaa acttccggtt gctgtctgac tccgcctgtt ctgcctgccg tttcatcagg
    12721 gcgagatgct caatacgctg cagggctgac agttcagaaa gcgtgacggt cacaccgtta
    12781 tgttcaaatg attcggtttt caggaacatc gctgactctc cggattaact ggcggtgacg
    12841 gtaatttctg caaccgcagc aaactcacca ttaccggata caaccggaat gttgaccttg
    12901 cctgcagcaa cacctttcac ggtgatggtc ataccactga ccgacacggt ggcttttgtt
    12961 ttatccgctg acaccgcacg gaagctcttg tcggttgcgc cttccggctg gaatgccacg
    13021 gtcagcgtgg tgctctgccc tttcaccacc gaagtgctgg caggcgtcac ggtcatgccg
    13081 gttgccgctg ttaccgtgct gcgatcttct gccatcgacg gacgtcccac gttggtgact
    13141 ttcaccgtac gggtgatcac ttccttcgcc gtcaccgcct taccgatact gctgacccag
    13201 ccgcggaaca catcgaccgt gccgttcggg aagcggattt tataggcacg ggtatcccct
    13261 tcattaaacc acgccagcag cgcctgctgc ccctgctctc cgggcatcca cgccagcgtg
    13321 aagctggtat ctccggcaga tttctgcccc tgcccggtcg cagtccagtc cgcatcttca
    13381 tcatcgagat aactgtcgtc ataggactca gcggtcagtt cgccgggcgt caggtcttta
    13441 actttagcca gacgcgacca gtcaacgtct gaaagcgggt tcgcataagg gtcgccgctc
    13501 ccgttataaa cccacagggt ggttccggca cctttcaccg gtattgctgg atttggtaca
    13561 ggcatatcgt cctcacattt cataggtaat gacatacgtc agatcggctg aactccacag
    13621 gcccgcatca tcgtcgcgcc ggtagtcata gccactggcc accatactgg tgatcaaatc
    13681 tgacagtgcc gggatatcgc tcatcaccgg ataaatccgg gactccatcc acgcatccag
    13741 ctctgaatcc ggcacctgag caggcaggaa aacttcgata tgcagctccg cctgccaggt
    13801 atcgctgtcc agctcttcgc ccgtgtattc agcgccggtg agataaacgg caactgccgg
    13861 aaaatccgcc tcatcaaaaa cagcggggcg accatcaaaa aacgtcgccc cggtgtcatg
    13921 cttctccagt gcatccagta cggctgcacg gagttcagta tgtttcatcg ctttattacc
    13981 atcctcagtt gatgctgcag cgcatagccc agctctttcg gaagacgctc acgccgtatc
    14041 cgctcaatat tctgtttaaa cgccgtggtc agcggcaccg ccatcggaat tttcaccaca
    14101 tcaatggggt aacgattttt cccggccaca cgctgcataa catgccagcg gccatttttc
    14161 agttgctgaa taaacgcgcc gggaatacga cggtttccca ccacaagcac gctgccgcca
    14221 cctttcaggg ctgaacgctg ccccttttta cgacgcctgc gtcggggcag gacaatccgc
    14281 gcgttaccca gctttattac gggcaaatcc ccccggttaa ccttgattct ggcctgcgga
    14341 tttttgaccg tggccctttt cagcctggcc ctttccttta ccagtttccg gcgtaccttt
    14401 gtctcacggg caacctgtga cgccgactgc gatatcgcgg atgacgcaac gcggttaatg
    14461 gccattgcgg cggcaccggg aaccgccgtt ttgctgatac ggctgaggtt ttcaacggcc
    14521 tgctcaagac cttttatgac catacatccc cctttcaacg ccgacggtta acggcaggcg
    14581 gaacgccacg cccaagccag agatgacagc ttccgccatc atccggcgat acccggtcta
    14641 tccagaagtt ttcctcaccg atggtcagcg tgtctccacg ccgcagctgc cgcacctcat
    14701 cagtccggac aaacagggac gggctggagc cttcaacgcg cacgccctgt ccggcatagc
    14761 tgatattttc agggtcatca aaaacaccac gtattactgc gccggactgc tcaccggatg
    14821 tcatggtggc tgacgttccc atgtacccgc gtatcgtttc atcggcgcgg gcaatggcag
    14881 catcgaacag gttatcgaaa tcagccacag cgcctcccgt tattgcattc tggccaggcc
    14941 acgttctgtc atttcagctg ccacaccggc agagacacgg aacgccgttc ccggcagcac
    15001 aaatgccaca gcctcatccc gcgtggcgtg aagtgcatca gtatgcagcg tcaccagtgc
    15061 cacaaccgtg accagatcag ccgtatcagt cacggtatcc ggctgcgctg atacaacctc
    15121 attttcatgt ccggtcagcg cattttccgg gctgacagat gtgtcctgac cggcagcgtc
    15181 atccgtgcca tcaagctcct cttccagctc tgccacacgg agtgccagtt cttctttcgt
    15241 ccccgtcagg ctgacatcac ggttcagttg ctcacccagc acctgaagac gggcaatcag
    15301 ttcatctttc gtcatggact cctccacaga gagaaaatgg ccccgaaggg ccatgattac
    15361 gccagttgta cggacacgaa cgcatcaggg tcagccagca gcatcagcgg tgctgactga
    15421 atcatggtga actcacgcgc cggatcgccg gtggtcaccc agtttttcgg gtagcgggca
    15481 gaggcgttaa taccttcgcg ctgtgcgtcc gcatcctgaa tgcagccata ggtgcgcaga
    15541 ccgcgtgcct gagtgttccc cagcaccatc gtgttgtccg gaaggaagtt ctttttgacg
    15601 tcgttttcca catactgtcc ggaatacacg acgatcgccg tatcgccata catcccctta
    15661 taggacaccg cttcgcccag gtcttttacc gctgtctcca gctcggaatt agagccgcga
    15721 cgggtatcca gcttctcctt gacggctttg aaggaacgga acagcgccca gcctttcgga
    15781 tcaaacacga tgatattcac cacaccgctg gcgttcagcg cgtaggcttc gatatcgtcg
    15841 gtcgggtcat acgtggactt gtcacgcttg ctccactccg taccgccgga ctgcgtgatg
    15901 ttgttcgcca cactgcggcc catatccact tcaaccggat cgaaggcttc accggtcatg
    15961 gtgtatttgc ccttaagcac ggcagaaacg gcctgcatct cttcgacctg agcaatggcc
    16021 agctcttcgt ctcgcatgtt ctgcaggatg atgcgacggc ggcggtaagc cgggtccgcc
    16081 agattctgtg gatcttcatc cggcagacga cgcagggtca tctgcggatt cacctcatgc
    16141 ttgggtttga catatcccgg tgtaaattca gaggtggagc cgccacggga gcggataacc
    16201 tcaccggaaa caatcggcga aacgtacagc gccatgttta ccagtcccgg aatttgtgag
    16261 agatagactt tctccgtggt gaagggatag ctttcacgga aaaagagacg cagaaacagc
    16321 ggatcaaact taaatttctg ctcatttgcc gccagcagct gggcggttgt gtacatcgac
    16381 ataaaaaaat cccgtaaaaa aagccgcaca ggcggccttt agtgatgaag ggtcaggtta
    16441 aacgatgctg attgccgttc cggcaaacgc ggtccgtttt ttcgtctcgt cactggcagc
    16501 ctccggccag agcacatcct cataacggaa cgtgccggac ttgtagaacg tcagcgtggt
    16561 gctggtctgg tcagcagcaa cagccagaat tccaacggca gcaccgtcgg tggtgccatc
    16621 ccacgcaacc agcttacggg tggcggtgtc cagcatcagc ggagtcattg caggcgcttt
    16681 cgcactcaat ccgccgggcg cggttgccgt atgtgccggg tcactgttgc ccagcggctg
    16741 gtaatgggta aagatttctt tgctcgtcat aaacatccct tacactggtg tgttcagcaa
    16801 atcgttaacg gcatcagatg ccgggttacc tgcagccagc ggtgccggtg caccctgcat
    16861 cagacgatcc agcgcagtgt cactgcgcgc ctgtgcactc tgtggtgctg cggccagaat
    16921 gcggcgggcc gtttccacgg tcataccggg ggtttcggcc agcacgcgtg cctgttcttc
    16981 gcgtccgtga gcctcctcac agttgaggat ccccataatg cggctgtttt ctgccgcaac
    17041 cgctgcggtg atctgcgcgt tcacgtccgg ctgcgcagcg ctggcgttct cgccctccgt
    17101 cgcttgcacc acgccagtaa cgtcagcctg cgaagcagtg gctgaaacag ttgttgattg
    17161 agtctctttg gtcattcgcc ctcctgagag acgggattta cgtgcatcca gtgcatcacg
    17221 catgacggtg atcgcatcgg tactgttaac aagttcatca gccagtccgg catcaatggc
    17281 ctcctgaccg ctgtacactg cagcctcggt atccagcaca gcctgcacgg acaggccggt
    17341 atatgccgac accttctgtg caaacatccg gcgggttgca tccatccggg actgcagtgt
    17401 ttcccggaca tcatccggta gatggctgta ggggttgcca tccaccttat ggctgccgct
    17461 gtaaatcagc gtgatttcca cgccctgttt ctccagcgca gcaccgtaat tactgtgagc
    17521 catcatgacg ccgatggagc ctgtccgggc ggtctgcgtg accagacgcc gggaggcggc
    17581 actggcaagc agctgacctg cgctgcagtt catgtcattg gccagcgccc ataccggctt
    17641 tatgtcacgc acacgggcga tgatgtcagc gcagtcaaat gcccctgcca ccattccgcc
    17701 gggcgtgtcc atatccagca gaatgccgtc caccatcggg tcgctggcag cctgttgcag
    17761 acgggcgata atgccgttgt aaccggtcat ccccgaatac ggctgcagcg cccgcgtccg
    17821 gctgaccagc gtgccggaaa ccggcagcac ggcgatgccg ttcatgacct gataactgcg
    17881 ggcctgtcgt ggtccgtcat cattaacgga taacgccagc gtcgcgggtg cctctccggc
    17941 agtcaggctg tcaccggaca ccgcatccgt caggcggctg atcccaagct ggcctgcaag
    18001 cgcacaaaag aaaacccgcg cataggcggg ttcaagcata agcggctcat taaaggccat
    18061 gctggcaata tgcgggagat tacgcagctc tgctgtcact cttctcctcc tctgttgatt
    18121 gtcgcagtcc ggattcaaat gccgcagccg cccaggcggg cggtttaaga ccggctgcac
    18181 ggcgctccat cgtttcacgg acctgctggg caaaaatttc ctgatagtcg tcaccgcgtt
    18241 ttgcgcactc tttctcgtag gtactcagtc cggcctctat cagcatcacc gcttcctgta
    18301 cttctttcag accatcgatg gccatacgac cggagcctat ccagtcacag ttcccccagg
    18361 cacttcgggc ttcctgaaaa ctgaagcgcg cttttgaagg taacgtcacc acgcggcgag
    18421 cgatggcctc ttccagccag cacagaaaca tctggctcgc ctgacgggat gcgacgaatt
    18481 ttcgccgccc cataaagtac gcccacgact cgttcgcact ggcccgtgcc gtggagtagc
    18541 tcatctgagc gtaattccgg gaaagctgct catacgagac acccagcccg gcagcgatat
    18601 accgcaacag tgactgctca aacacggagt agccgttatc cgtgtcctga gccgtctgca
    18661 ggttcagtga gtcccccggc atcaggtgcg gcacttttgc gcctcccagc cggaccggtg
    18721 ctgcggcgta atacgcggca atttcaccaa tccagccggt cagcctgtcc cgctgctcct
    18781 tactgttcgc gccaagaata aaatccatcg ctgactgcgt atccagctca ctctcgatgg
    18841 tggcggcata catcgccttc acaatggcgc tctgcagctg cgtgttctgc agcgtgtcga
    18901 gcatcttcat ctgctccatc acgctgtaaa acacatttgc accgcgggtc tgcccgtcct
    18961 ccacgggttc aaagacgtga atgaacgaag cacgaccgcc cggtaactca cggggtatcc
    19021 atgtccattt ctgcggcatc cagccaggat agccgtcctc actgacgtaa tatcccagcg
    19081 ccgcaccgct gtcattaatc tgcacaccgg cacggcagtt ccggctgtct ccggtattgt
    19141 tcgggttgct gatgcgcttc gggctgacca tccggaactg tgtccggaaa agccgcgacg
    19201 aactggtatc ccaggtggcc tgaacgaaca gctcaccgtt aaaggcgtgc attgccacac
    19261 cttcccgaat catcatggta aacgtgcgtt ttcgctcaac gtcaatgcag cagcagtcat
    19321 cctcggcaaa ctctttccat gccgcttcaa cctcgcggga aaaggcacgg gcttcttcct
    19381 ccccgatgcc cagatagcgc cagcttgggc gatgactgag tcggaaaaaa gacccgacga
    19441 tatgatcctg atgcagctgg atggcgttgg cggcatagcc gttattgcgt accagatcgt
    19501 ctgcgcgggc attgccacgg gtaaagttgg gcagcagggc tgcatccaca ctttcactcg
    19561 gtgggttcca cgcccgcaac tgcccaccaa atccgctgcc accgccgtga taaccggcat
    19621 attcacgcag cgatgtcatg ccgtccggcc ccagaagggt gggaatggtg gacgttttca
    19681 tacataaaat cctgcaggtc ccctgcgtcg ctgtgtcatg ccggtctgca cttccagctc
    19741 cgcaatgtat tttttcaggt cagacacgga agtggccgta aactccactc tccgtccgtc
    19801 tttctgtacc gttgccaccc gttttcctgt catcaggtca tgcagtgccg cacgggcagc
    19861 ggcaagttct tcctgtcgcg tcattcatcc tctccggata aggcacgggc gtaatctgcc
    19921 agtgttttct tgttggttgc tgcactatcc tcttcctgca gactcgccag cagcgcactg
    19981 agatccagct gccagcggga aatactgata cgcagcgccg ccagcgcata aacgaagcag
    20041 tcgagtgctt cattgcgtcg ctttttgctg tcccacagta tttttttcct gccatccacc
    20101 catttttcga cctgctcttc agccgtcagc tgctgcgctt cggtcagatc aaaaatatcc
    20161 gggttattcg ggaagtgaac ggcaccggga agcggttcat ccccttccgg cgtcagtgtg
    20221 aagcggttat aaatctgctc tttcgcggta tccgtaccga tttcggtaag gtaaaccccg
    20281 tttttgtttc gcttacgtgg catgctggcc accggctttc cgtagacgga tgccccttta
    20341 atggggatca cccggaacag cccatgtttt ttcgagcgtt catacacaat ggtcgggtca
    20401 atcccgccaa tatcccagca gatacgggat atcgacattt ctgcaccatt ccggcgggta
    20461 taggttttat tgatggcctc atccacacgc agcagcgtct gttcatcgtc gtggcggccc
    20521 ataataatct gccggtcaat cagccagctt tcctcacccg gcccccatcc ccatacgcgc
    20581 atttcgtagc ggtccagctg ggagtcgata ccggcggtca ggtaagccac acgatcagga
    20641 acgggcgctg aataatgctc tttccgctct gccatcactt cagcatccgg acgttcgccg
    20701 attttcgctt cccacgtctc accgagcgtg gtgttcacga aggttttacg ttttcccgta
    20761 tcccctttcg tcttcatcca gtctttgaca atctgcaccc aggtggtgaa cgggctgtac
    20821 gctgtccaga tgtgaaaggt cacactgtca ggcggttcaa tctcttcacc ggatgacgaa
    20881 aaccagagaa tgccatcacg ggtccagatc ccggtctttt cgcagatata acgggcatca
    20941 gtgaagtcca gctcctgctg acggatgacg caggcattat gctcgcagag ataaaacacg
    21001 ctggcgggat catccggcgt ccatttgagg ccaaacggtg tctctttgtc gccaaattta
    21061 agatactgct cctccccgca gtgcgggcag gcaacatgaa aacgcataaa atgcggggat
    21121 tcactggctg cacgctcaat ctggcaggtt cctctcactt tgggcgtgga gccacggatg
    21181 gactttggcc agaccgagcc ttcaatacgc ttgtcaccca ggaacgtcgg agagccttcc
    21241 tgttcaatat catcatcaaa agcagcaagt tcatcataac ccgccacatc caccgacttt
    21301 tcacggtagt tttttgccgc tttaccgccc aggcaccaga aaccacgacc attggtgaaa
    21361 cgcttcatgg tgagcgtgtt atcccggtgc tttttgccat accacggggc cagcgccagc
    21421 agcgacggaa tatcacggat ggtcggctcg acgtgggttt tcataaagtt ttcggcatca
    21481 ccatccgtcg gcaaccagat aagggtgtta cgctgcttat gctctatgaa gtaggcataa
    21541 acacccagca gcattttgga ataaccgaca cgggcagact tcaccacatt cacctcacgg
    21601 atgtagtcgc tgcccatcgc attcatgatg gcccgctgaa agggcagtgt ttcccagcgc
    21661 ccttcctggt atgcggattc tttcgggaga tagtaattag catccgccca ttcaacggcg
    21721 gtctgtggct ccggcctgaa cagggcacga agcccggcgc ggacaaaatg ccgcagcctg
    21781 tcaacctgac tgttcgatat attcactcag caaccccggt atcagttcat ccagcgcggc
    21841 tgctttgttc atggctttga tgatatcccg tttcaggaaa tcaatatgcc ggttatccag
    21901 ctcaggaaaa cgtcgctgca ccgacagagg aataccgtcg agaatactgg atatttccct
    21961 tgctatacgc gacagcacga aagtacagaa tgcggtttcc accacttcag ccgattcttt
    22021 ggcatttttc agttcctgcg ccgtcgcctg agcacgagtc aggcgatggc gctcaaattc
    22081 aagtgttcct gggtgaagat ctgcctcgct ggccagccgc agttcttcaa cctcccggcg
    22141 cagcttttcg ttctcaattt cagcatccct ttcggcatac cattttatga cggcggcaga
    22201 atcataaagc acctcattac ccttgccacc gcctcgcaaa acgggcattc cctgctcctg
    22261 ccagttctga atggtacgga tactcgcacc gaaaatatca gccagctgct ttttgttgac
    22321 ttccattgtt cattccacgg acaaaaacag agaaaggaaa cgacaaaggc caaaaagccc
    22381 gttttcagca tctgtcgttt cctttctttt cagggggtgt tttaaataaa aacatgaagt
    22441 tacggcgaag aagaacggaa acgccttaaa ccggaaaatt ttcataaata gcgaaaaccc
    22501 gcgcgccttc cgccccgtag cctgccggat cgccggaaag gacccgcagg cgttctggtt
    22561 tattcgcaat gaattcaatg tctgacagac cagcgtcgtg cgaccacggt cgcacgctcc
    22621 tgaatacatg ccctgtttct tccacccccg cacagagctg gcgagcatgg gggacaaacc
    22681 cgcgaatcat tagcgctgta aaaacccggt atgcatcgtt tttgattatt tccacacact
    22741 cgcgcagagg agttccccgt caggctacgg tcatagttaa tgcggaaata cggcgacgat
    22801 gcagcgcata atgtgtcagg cttgaatacc tttatccgtt aaaagggata ttagttaagt
    22861 tatcccgtgt agggtataag ccattatcaa agccactcag tagcgaatgg cttttgtaat
    22921 gggctattta attagtgatg ctgcgtattc gattagatat aacttaggtg ccagccagat
    22981 ttttaaccat gtcatattgg ctactataga aataataaaa cttaaccata gaataaaaac
    23041 tgctactaat ggtaagacaa aagcacttaa cctaccgtca ctttcccaaa tcatagtcgg
    23101 tttgtattta ggctgtccat tatcacatga gcgtccgtca ttatcgaact taccaatttc
    23161 aacttttttg cactgtttct tcataaacca aataaccagc gggattgttg aaatggctat
    23221 taatgtttta atcagactat caactatatt ccataccagc aactgatgaa caacatccgg
    23281 aatctgagcc tgactgaatg caatggcgga atcaattcca tcgctggcat tgttcaataa
    23341 atcaacaaga attttctttg cttcttcttg catgtatata tcctgtaagt tgtaagcctg
    23401 ttcgaaaaaa taaacaacta cgagagacag aaaaaaaaat atctcaggct ctctttctat
    23461 aagcttttag ttgaggatgt tctgcaaaac aaaactggtt agttatgtaa ctgcttctgg
    23521 aatacaacag ccgacgtgct acaacgtaaa attcgtttga ttgtggcagt tgaggaaaca
    23581 gacttgcatt tctcaatatc atcaatgatg accacatccg gtacaggcat gatgcgggta
    23641 tgcaatgttt ttgcactaat aacatcacag ccattcagtg gacgaatatc tgcatcttca
    23701 ggcactacca gaagcacacg tttctccgtt gcagaaaagc cagcagcaag agttcgcgac
    23761 aataaagtca ttccactacg tggcttcata caaatttcag aaatatgatt tttttcgtcc
    23821 aaatgatgct ggccatcatt tttatcatca ctcaacaact ctgctgttaa tacaatccct
    23881 gctgtctcag atttccagga aatgctgata tcctgatcat ctgcatcaac atcatatttc
    23941 atcgagtaag gactaaagca acgtccaacg aacgcatcgt ttaccagtac acgcccaccc
    24001 tgtctgacct gaaagtcaac gcgccctgaa agctgattta ctgttacaag caatgccttt
    24061 tttctttttg ttttattact cattgccaca aactgttcag ccatctctct tgtcacacta
    24121 ccagaaactc tttcttgaat tttcacagta aattttctgg atgtagtagc atcgacatta
    24181 aaaaatctgg atgtcattag gcagttatta acaattggca tcagctcact tacgattgta
    24241 acaaattccc ggtaaagctc attttgcttt tcagcacaca gttttccgca atcggccagc
    24301 ttaagctcaa gagcaattaa tcgttccatt attgaattaa ttttttgcaa ctctttcatc
    24361 aaaacctcct gacaaagata ccgtgcgacc acggtcgcac gctcctgaat acatgccctg
    24421 tttcttccac cctcgcacag gactagcgag catgagggac aaacccgcga atcattagcg
    24481 cggtaaaaac ccggtgtgca tcgtttttga ttatttccgc acactcgcgc agaggagttc
    24541 cccgtcgggc tacggtcata gttaatgcgg gaatacggcg acgatgcagc gcggaattta
    24601 gcgagcgacc acgatcgcac acgagaaaca aagagatttt tatactgcag aggaacaagc
    24661 tggcgggtaa ttcagcgtag tgaataccgg catctccatc ttgtatgcgt aatgatactc
    24721 agcagttgct ccagcagacg ctttccagcc aggaagcatc aaaatagcat ccgcacaacg
    24781 aagcattgca aagcaaatat ccatgtattc acgttgtgtc agaccattag gcaaagtggc
    24841 tggattcaag acagagtgac catgtcgtga caaacgatct gcctctttat taaaggcgtc
    24901 acgattaaag ttctcatatc ccgtcattgg cccggcaata taaattttca ttcactatcc
    24961 aacaaattga gttcgttttt gatacagagg gattaattca ggcattgcgt attgatgtat
    25021 tcctgaagcg ttctcagtgc tgtttggtcg cggataattc cgtcccggat accgagaacg
    25081 tttcgtccag caactgaaga gagttcgacg gtggcatcat tgcccatgcc ggaggcgctg
    25141 gaggttttgg ctgaggctgg cacagggcat tttcctttga cgagcacccg accaccatta
    25201 tcaagcttac gccgaagagc atcattttca gctttcgcat tggctaactc cttcgtgtat
    25261 ttagcatcga gtgcatcagc agcacgctgg cgctgctgca tgtcagtaat ggtggcggtc
    25321 gcctgcttca gctctctggc gtttttgtcg cgctgctctt tgtaggcgat ggcgttatca
    25381 cggtaatgat taacagccca cgacaagcag acgatgatgc cgataaccag agcggagata
    25441 atcgcggtta ccctgctcat tgttgacccc acaaacagac ttcacgctca atctcacgac
    25501 gagtcatcag gcctttccat tgcttaccgc cagcatatat ccagcgacgt agctgatcac
    25561 atgcgccttt gatatcgccc tggtttattt tgcgaagaag cgtcgatgtt ctgaaattac
    25621 cagcgcccac gttgtaaacg aacgagtaaa gagcgccgcg cgttgtttcc ggtatatcga
    25681 ctttgatgta cggattaatt tgtctggcaa cagtggcaag gtctttattc aggagagctt
    25741 tgcattctgc ttcggtatac gttttaccga gcatgatgtc ttttccagtg tgcccgtaac
    25801 atacagtcca tacgccaaca atatctttgt atggtatgta actgacacct tccagaccat
    25861 cgttaccact tgggccagtg atgagcatag acgctatggc aatagccccg ccaccaatag
    25921 cagctgtgac agctttgcgt aatgatgact gcatcatatt tccttcggtg ctctcttccc
    25981 cagctctcca ataaccatcg ctgtagcaga aagattattg gtattagttt tatccaatat
    26041 gtcctgcaat atcttcgtgc gcttcatctg ctcgcgttta ttgagccggt atgtaagcac
    26101 accgaggata atgctgaacg cgacaccgat aatgaagccc caatcctgca atgacaggct
    26161 ggcaaagaac gcagcaagac cagcgctacc ataagatgca ctgctgtatc gttcatccat
    26221 cttcatatct cttacctcgc aatagttgct gaggtttcgg cttggaaact atatggtgaa
    26281 aataacccct accaaaaacg gcaggggaat atattctcca gttactcaaa taactttttc
    26341 acttcatcaa ctgtctgaag ataacgctcg ccctcaatct caatacctat tgcctgacgt
    26401 cctaacttca gcgcagcttt aatagttgag cctgatccca taaagaaatc ggcaacgata
    26461 tctccggggc gtgaactact ctttataata tgttccatca tcgctgcagg tttttcacat
    26521 gggtgcttgc caggataata ttgcacagga ggaaactccc atacatcggt ataaggtaca
    26581 tcagcagtaa catgaaatgg acggcgtaaa ctttcatact gagccttcaa atcatcatac
    26641 tgcttcttta cttcctcata atgcaatttc agatcgctat aacgcttttc atgttctgaa
    26701 taatcaacat taaaaggata tggacacggg acaccgagtg aatctgcctt cctgcgaaat
    26761 aattcgcata gtttattaaa atcaattaat gatggaagcc gccactgcga ataagaaaac
    26821 cagtgtgaac acatctgctt tcctgtcgct gaattaatgt ctgcggctga tatatctaac
    26881 tgacggcgag ccagcgaaaa agcatcaatt agtggagaaa aaatattttt tcttaaatct
    26941 gcacattttg aagcataacc tgactgacct ttagcataac ctgacgcgcc gtaatgctct
    27001 gcaaataata ttctttcagt agcaggaaaa taagccctta aactttcttt atgacaaccg
    27061 cgccacatac cactgggttt agcccaaatg atatggttca gaacattgaa acggtcgcga
    27121 gttaataatt caatttttga cgcacggcga gagccggtaa acatatacag gctgccatta
    27181 ggggccaata tccgccaaaa ttctgccaaa aattcgtcaa tccaccccaa aaagtcagca
    27241 tcgctatccc actggttatc ccatgcgtta gcctttacgc cgaagtatgg tgggtctgtt
    27301 gcaatcaaat tgaccgagtt atcagggatg gttttgataa attgcagaga atctgcgcaa
    27361 acgagttgtg cgccatgaat attagtggtt ttgaacatag ctattatttt tttgcctggg
    27421 taagctaacc cagcgatgcg catcgcgggt gggctttagg ttcagcctat agctctggca
    27481 tgggttgacc gcaggacaag ctgcaacttg tcctgcgccc acttttcagg cacaaaaaaa
    27541 ccgccataaa ggcggcttgt tggctattgg gtacaaaaaa cccaacttaa caaaacaata
    27601 actaaaaacc gtcattttgc caagcttttt cgtttttgtg ctgtgcgacc gtggtcgcac
    27661 agtttttaga aagttgcctg cttatattca tcggtcaaag aatatcgacc agacccgcta
    27721 cgctttgcaa ccccaaaaca gatcatctgt tcgatgataa attcaacggc agcctgactc
    27781 aaaaaacagg cttcgcttaa ctctttcaag gaaatgcgtg gatatccacg cataacacat
    27841 tccacactca gggccgcttc ggtcatattt ccacgaattt cattaatatt cataacgcct
    27901 ctctaatcaa tcttgaaact ggaaatcaac atcagccata aagcggttca actcagccag
    27961 ttttggcccc atagtcccaa ttaagcggcc agccagtcgg tctgtcaggt tggtactgtt
    28021 gaaaccatat tccttttcga aacgttcaac cttctgccac aactcataca attcgttagc
    28081 tatctccgca acgtcgccgc gcattttttc gttaccttga taattcataa tatcctcgaa
    28141 attcatatag ttagtgggtt atccccctca acaacacgaa ctgtaactct gccaaaagaa
    28201 cacatccagt gttattttca ctttttagtg aaattttata tttgctttgt tttctcgttt
    28261 tggtgtattt tgcgcgttag gaggctataa tatgtttaac gtgataaccc accctgcagc
    28321 actggatgaa ctgcttgaac taccagacga tctgcgaggt cgcatgacca ggctaattga
    28381 acgactggag aaagagggaa acaaacttaa gatgccccat agccgtgtaa taggcggagg
    28441 actctttgaa ctaagagtag gggataaaaa catcgcaaga acgctatacg cctacgcgac
    28501 tggtaacgaa atttatctat tgcatgcatt tgtaaaaaag acacagaaaa caccagtaaa
    28561 ggctatagaa atcgccagaa ctcgcttgaa ggagatgaac tgatgaaagc aaaaggcatc
    28621 ccgtttaacg aagtaaagga aaaactactc aacactccag aagctatcaa aggatatgaa
    28681 gaggccgata aagaactgga gatggtcgaa atgttatacg aaatgaggga aagagcaggt
    28741 ttaacgaagt cagctctggc agaacgaatg ggattgcgcc cgtccgccat cagccgcctg
    28801 gaaagcaatc ctttaggggc aagcatgaaa actttatcaa aatatgccca agcatgtggc
    28861 gcaacaatta atattaatgc cgtctattga ttaatcactt ttcaccaccc cgtccagaag
    28921 ggcggggtgt gtatcaatga tgtaaacact ccacagcaat cgcttgttgg caattctgag
    28981 caatctcccg cgacatacta acaagccgtg aaatctcgcc aagatgcaag ctagcttcgg
    29041 gagaagccag agcaacgcta accagatcca aaacggcgtc gatgtctttt aggtttgatc
    29101 cgagttcttc aagagttgat acagatgctt cagtcattga atctaatacc atttaattaa
    29161 aaaaggttat ttgatactgt ataaaattac agggtaaaac tcaaatttta tagccaactt
    29221 tcaatggatt aacagggtta atagctttgt agtccgatac ttatgttctc ttcctacgcc
    29281 tcactcaacc acctacagaa gcccatcatc accataagaa aaactctttt cgcttcgttt
    29341 tctcatcatt tcaaaaacta tgcctttttg tttatttatc attccgtgac cagtcacact
    29401 ttgagcacaa gtttcagcct cgtagttgta aggtgacatg agccttcacc atcaaaaata
    29461 cattctgaaa caggcatttt ctgaccacat cgcgggcaag tagtagatag ctgcttctgc
    29521 agctccttat agtttttcct gatcagcaga ccgatgactt cattctctga atatggaact
    29581 cgtcccggtc tacgcaatac acaaatttcc cccaacatac gtagctcgtc aggttccaga
    29641 atccagtccc gtcttgatgt gccttccttc ttcaaccgtt ccctgcgtct cctttgccgt
    29701 tctgcagctg tcagcgccat aaatcctcct gaaaattact taacccaatt aactacaccg
    29761 ccagcggcca caaccgcttt ttcacagtca cgctcatatc tcaaaggttg aaatacacca
    29821 tcaataaaat atttatctcc accacatgct gcaggaagtg aaacagaaaa ctgaatatga
    29881 ccatccggag ttaccggaga gttgccagcc tcatacgcga cccttatcca gtgcataagc
    29941 atttctgtac ttacacatcc acaatcaaca tcaattttgt catgctgctg ttccagccat
    30001 tgctcaaaag ttaacttgta agtttggctt acaggtttgg cttccagttc tgctatgtga
    30061 tttttggctg attccaactc aatcgacaat ttttccaact gctctttatg cttcttgtat
    30121 tcctgatatg cgtgccaaga ctgacctttg cgcacactat ccgtgatatc agtaatctgt
    30181 tctggtgtta gcgtggtcag tggctgtgct gggaaaatca gcactttccc ggaatcccaa
    30241 tcaaaaccag cgtgaattga ctgaacctca actgaaggtg ttgaaccaat gctgccaggc
    30301 gaatgaacaa cgatcgttac atccatatcg cgacgatcgc tgtggttgtt ggacaaaata
    30361 cgattcaaca aaatacgatt caccaactca gaaaatttag aaaatttcat gctgattcac
    30421 ctttctctgc tctctcctgt cggaacatca ctatcatcag gtcgcctttt gtcgctatct
    30481 tggctgttgt gcctggctca atgcggctaa gttcaaatgc gtcatagaac gcctccaacg
    30541 ctttctggcg tttttcctgt ttgcggcgct ttccccactg ctttagaaaa acagaggccg
    30601 cccacttccc cgcactgaac atgatgtaaa accaaccgag aagtgccaga ccggtattta
    30661 gggccgtatc gatcgttatc gtggtgtcga tattcactac gccacctcct gaaaatttcc
    30721 ctgataaaac gccaacacac gctgcataac ttcgctcttc cgacaatcgt gacaaatcat
    30781 gttcaagcgc ctgtcataac gacgtatttc tccatcaggt aatgaccaga taaggtcagg
    30841 atcaacaact accgatttct tcggctttac tcttgatagt tttttacggg cgttttgcca
    30901 gtccttgcgt gcttgtgcag acagaaatat cccggagcca gagatataca aatcaccact
    30961 ggcagcaagc tctcttgata aacgactaac agcacaccgg ctaataccag tttcatccgc
    31021 aagttgccta accgttcctc gtccattcag gcgcacgagt tccacaattt gtttattcag
    31081 ttcttccctc tgttctggtg taaaagcctt tgccattacg attctccttg cccttttaca
    31141 agacgggaga aaattgcgga tacatattta gcctgatgaa tagcatcagc cagggcattg
    31201 tgtcgttcgc catcaaatgg catatctttt ttaggattaa aaccaataaa ctgaccaaga
    31261 ttaacgattg ttcgaacatc ctgatcatta aaatattccc acggacaggc taagccaacg
    31321 cgctcataag caccgcgtaa aattacatta tcgaacgttg ctccatttcc ccatactttc
    31381 aactttttta aattacatgc atggcaagta ataaagtgtg ataattcaaa aagtgcatcc
    31441 ttaatatcaa gggcatcatt agtacaaata gcggctctgg cttccttgct ctgtttcaac
    31501 caccataaaa tggtatcacc atcggcgata ccaccattca acatcgagct tctgagattc
    31561 actggcacat aaaaagtagg acctaattcc tcactttctg gagaaaaaaa cactgccccg
    31621 atagaaacaa ttggagcaca tgtgttagta cccattgttt caaggtcaat cattacatca
    31681 gaccgtttcc tgtcgtaaat aatctgatta gtcacgaata attctcctca acgtttatct
    31741 atacccaaat tttctgttga tatgttctgg atggttttgg cgttctttct gattcaggta
    31801 aatgaacata aacgcaatat ccatcaccta aaaaatcaga acgaacaacc agaactttct
    31861 taccacgccg ccgataagta tctgaaagac gctctgcttc atcatgcgtc atcggcccct
    31921 gcttaaatgg cgttattttc attttcttgt gcgaccacgg tcgcaccccc tctgatttcc
    31981 aagtaacgct taatccaaag attttctata tgcttattac ctggctgatt tgataaatac
    32041 caatcggcaa ttacagactg cttattacta tctggtcgtg tcctgtaacc acaagttgga
    32101 caccaaataa catattcatt tcttaccccc gaatacctta attctggctt cgacggtttc
    32161 ctgaacataa cttgtttaca aaggcagaat ggcacttcct gcccgatggc gtttgatgat
    32221 ttcactgcgc ttctccgccg catttaataa tgcggttcga tttaagctca gacatcgcga
    32281 cttaattaaa agccagcgtt ctttataatc ttgtctccag ctatcaatag atatattcag
    32341 tagaaaactc agatgcctgt cctgttctct ctcatcaact gcctcagcac acgtaagttc
    32401 tgatttaacc tgttgtgccg cgtaataaat caatttccgc atggcttttt tcgtggaggc
    32461 cttcattttt ttaaagccgc cccccgaatg agcaaccaga aaatcaagcc ataaccactg
    32521 acaaacaatc acatcattgt tgtaatcagg cttgcatccg tagcaataat aaagccaggc
    32581 tgattcctcg ttattcaact gaccaatagc cctgcgccag ctggcgctct gaaaatcaat
    32641 atcagtgagc aacattgatg attgtttaaa tgttttcccc tgatggaatc taaccggctc
    32701 agcaggaacg gacacttcgt atgagttatc atcccccaca ctgattgacc ggactggttt
    32761 attgttaaac cgtcctgatc taccgagacg catctgctca agctggacct ccagaatgcc
    32821 acgctggcga taatgaacat cagccaatgc cgtagagacg cagctgcgta tatactcaag
    32881 ctccacgctt acgactctcc ttaatcgact gctttacccg ccgagttgta aagtcactct
    32941 tcagcttata agcagtacga acttcaatat cgctttgccg caatgaagga atctctccag
    33001 cggttaacca ttggtaaaca gctccgggtg ttacacctac ccctgccgct gctttttcta
    33061 cactgccaaa atggcgaata agttcttctg gtttcatgtt gttattataa acaataaaca
    33121 acaattaaag ccagctataa taaataaatt ttatagccag ctataagaag atcatttatg
    33181 attacagtat gaaaacacga ggcgaacgac tgaaagcacg ccgtttagag atgaagctga
    33241 cgttgcgaga agttgctgag gctgtcgata tctctatagc aggcgtccaa aacttagaac
    33301 gtggcgacgt aatgccgtca ctggagatag gtattgccct ggctaagtgc ttacgcaagc
    33361 cagtgcattg ggtgctatat gggacagaat atgaccctga tcgcattcct gttattggca
    33421 caactgaaag tgggccggat gaagagtgga agccgggaca ggttccaaat actgagcgat
    33481 tcctgccatt catcagccag cgcaatactg tgtatgcgtt aactatcagc aatcttacgc
    33541 agggaaacta tcagccaggc gattttctcc tggtcgattc tgcgctggaa ctggtccccg
    33601 gcgaggatgt tctggttcgt gatactgcag gaaatatcac tatccagcgc ctggcccgtt
    33661 ttgatgatga aaattactat ctggatggaa caaactcaca acgcgccatc ttcaggaaaa
    33721 gcgatcttga atttgttcac caaatagtcg gaacgatcaa atcattcatg gttgagggta
    33781 gatgacttaa tacggggttt attgtttgct gtaaatctgg tttaatgcag ggcatatgtt
    33841 ctgtgggttg actcaggtta cagcagcaga aaaaagacga aaaaaaaccc gagtcggcaa
    33901 actcgggtcc ttttcaggaa gcagccacag taacgcgacg gattccgtca attcaagatg
    33961 cgtttattgt ggctgctcct gcggattttt tcaacccgaa aaaacataat tcgcatggaa
    34021 tggctaaaaa atgacattaa acgatttcta tgcggatcgc tttggcagcg atccgttctc
    34081 gctaattgaa gcagcacggg atgaactaac cgagctggca cagatggctg gtatcaactg
    34141 gcctgcctgt tccgacaaca tccaactcaa ccctcgcggg ggggttgagc gttactcttc
    34201 atacaataaa acatctccag aagctctcga aaagagcctg aaaggacgcg tagaaattta
    34261 ctccaggctg gaacacagca aagggattga ttacccgttc attaacttcg tccataaagg
    34321 cagcgatgct ggctcatgga gcggtttctc cttcctgttt tctgaatatc gccgtgagca
    34381 acaacggaat aatgcgaccg tggtcgcaca accagaagaa gaacgggaaa gaatagaacg
    34441 tcaggccgaa gcacgtcgtc gccgtgaaga acaaaaacga attaatgacc tgaaaaacaa
    34501 ccaaatggac catgaacgtt tgctgggatg gctggcattc cacagcgcgt gggaacatgc
    34561 tcaggctgaa gacggctcat ggccatatgc gattaaaaaa ggtattcgtg acgtttttag
    34621 ttcatgtgat gtgcgtcgcg tgaccagtca cgacagtgcg aaatggagcc gggggccaac
    34681 cacatatatg gctatccctc tggctcacct ggacggtcgt tatgatggtc gtattgtggg
    34741 ttggcagcga atcgacaaac aaggtgggaa ataccagacc agcgcagtaa ccagcggtga
    34801 tttcgtcggt gcctgctttg ttatcggtga gctgaaaggg gcgcaaaaca tcgctgttac
    34861 agaaggtttt gctactggtg catccatctg gcttgccacg aaaaaagata aacgctttga
    34921 cgctgtggtt gtcgctgtat ccgctaacaa catggttcat gttgtggaac aactggtaaa
    34981 catgtacccg gcggcaaaaa tcacctgcgc actggataat gaccggaaat ctgccacaga
    35041 aggaaaaggg aataccggcc tgcggactgg ctatgaaatc ttatcgaaat tcaacgatgt
    35101 ccgctgcgtc tatccaactt ttgaagacga tccgcaaatg gagtgcagtg acttcaacga
    35161 cctgcacaaa ctacgtggac ttcgtgaaac agcacgtcaa ctatttgcaa cagcaaaccg
    35221 cctgaaaacc ggtactgatt tgctatctct gacgctcaac cgcctgaaga caattaagcg
    35281 ggataaccgt cgtacttttg ccaaagagct gctgagagcg gtggatattg gcatgctaac
    35341 ctgtccggtt cctaacagtc cgtcagattt atttaacatg ttctgcatcg ttctgcgtga
    35401 aatgggactg gaaaatatct atcgcgctac ggtaaaagac cacattgccc gtcgcctgaa
    35461 taaaaagtgc cgtattgccc aggctccgcg ctctttcagc gaacgcataa ctgatcccaa
    35521 taaacgccct caacatatca cctataagcg ttttgaaact tcggtaatga ccgatgatgt
    35581 cctgaagtac gtgcaatcac tacaggggat agtcatcgtc cgtgctggta tgggttccgg
    35641 caaatctact ggattgttac gaccgttaat gcatagctca gaacgtggta tctctgttgc
    35701 tcaccgtgtc tcccttattg gtggcctgtg ggaaatgatg acagaaggaa aaggtgtaaa
    35761 agccgatatc ctgcactatc aggaccctgg ctatcaggaa atggctccat acgccagtaa
    35821 actgaccatc tgcataaact cgattgttaa aaactgctgg caaccgttga tgcgccagca
    35881 cgactatttt ggctttgacg aggccacaca agggctacgt gccgttcttt ccggtcgggc
    35941 tatggaaaac ccggttgctg tattcaatac gctaattgac gcgctggcca gaacggaaca
    36001 acacccaatc atggtggatg ctgatgcgaa cgatctactg gttgacctgg ctgaactggc
    36061 tatgaaacgc cgtgaagaac taggtttacc ggcatggctg cagatccacg taattgaatt
    36121 acctgttgat gttcgcaacc gcgaaacagg tgagccaatc cgggtttttt atacagaaaa
    36181 agatcgcatc atgaccgagg tggtgaaagc tgtatcggat ggcgaaaaaa tcatgttggc
    36241 caccgacagc tccacctttg cagaagatgt gacagccacc ctgagaatgc attttcctgg
    36301 taagaaattt ctgtgtgtaa accagaaaaa caaaccagag ccagaagttg aagcatttac
    36361 caatcaaccg aaaaagatgg tcaaaaaata cgacggcctg atttatagcc cgtctatttc
    36421 atccggcgtg tcgattgagc aaaaacattt tgaccgtcat ttcggcatgt tctgtggtga
    36481 agttgtaccc agcgacgcag tgcaaatgct acgccgcgac cgtacagcaa aagaatttat
    36541 tatcggcttt gataaagctc gtgcaaaacg tgaaactgac ccgcaaaaaa tagaacgagc
    36601 attcgcccag gcattgcttg ctaccgcagg tatgaacggt gaacttacgg atgtcgtttt
    36661 cgatggcgac cgtatatcaa tgggggtggc caataccgac tttaccagga tgaaaattaa
    36721 agcagccgca attgaagcga ctgcgcgtaa cgactacgcc agcaacatga tctgcatcat
    36781 gcacagcgac ggttataagg tatcaccgct ggccaccgat tatgctgcga atgatatcgg
    36841 taaagatctg cgtaaagaag cgcgggatat tgtgtgggaa cagacactgg atctgcacct
    36901 caatacagaa actccacaag aatcagagcg tgaagaactg ctaaaaaaac gcgcgctgac
    36961 gcttgaagaa caggcaaaac ttgtccgctg ggatattgaa aacgaactga aactaccggt
    37021 tgaagaaggc acgctgaaat tctatttcga tggtgctcga gacaaagttc gccgctatga
    37081 aacaatgctt ttagatgagg taacagctcg tcgttacgac cgggaagaag cagccatcaa
    37141 ttttacctac gcataccgtc aggcgggaca gtggcaatat tttgtagtga ccgcgatgac
    37201 tcgcgagcag gctgatgatg tctttaaaac taaattccct ggaataatcg atcacaaggt
    37261 aaaagctact cctactgttg aagtcggggc gcgcggtttc tatgcgctta aatcagcaac
    37321 tctccggcag tactttatcg actgcggtat caaccctgag actatgaccg gcgaggcgac
    37381 tcaggagcgt ctcaaattcg ctcgagataa cctcatgaca gccgaacgcc gtgacctgtt
    37441 gaataacgtt ctgcacattg gtggttttat gacgccaaaa gggaagccta aagtgccgga
    37501 agcgttgttt aaaaacattt gcgactctct tggccttaaa acaggtaaac gtcgcggcag
    37561 agacggcgat aagcgtccaa ccttgcgttt tgttgagcct gaatcagcgg catttatgct
    37621 ggatattatt gttcagcgtc agaaagatgg cctaacccta aaaacgcgta aagccgagaa
    37681 gacagccatc gaaatggatc gcgatttgga tctcaatata tatatggatg ataaaacgcg
    37741 atccacaaac gagcaggttc aaaacgcccc tcattcagta atttctgagg ctttggccgt
    37801 gttgccagtg gctgtacctg agtcctgggc gatgagtgcg ctttccagtg aggagttggc
    37861 cactatgtca tcatggtcat cagccagcat agctatgacg tttgcatccc tgtacctcac
    37921 agagttcatg gatcaccttt ccagcaatga gctgcggcgg ttacgtgaat acattacggg
    37981 gaatagctca tgcagctacg aagagcaggg cgctttctat gggtaatgag tggatattat
    38041 ctttgtgtct cttgctttgt gtgttccagc aatggttttg gttcatagac cgaaagtctc
    38101 acaaggaaaa catgaaggcc aggaaggcga tgtttcgtgg tgaacaattt gcgcagactt
    38161 atcgtatgtg gaaacgtttc tatcgtgaat tttcggtgaa cggggagtaa aaatatgtct
    38221 actactgcat ttttggacaa acttgattac cgtcagctgc agttctgccg ggatgaatgc
    38281 gaacgacgca ttagggctat ccaggaggaa gaaaaaaagg tcgcctgggc tgtaacagat
    38341 cgtggcctta tctttggctg gttccgtacg gaggattatc tgaaagcagt ggaattattg
    38401 gcgcgtaagg ctgctgagcg ttgggaacga gccgacaagg aaaacccgga gacgaagtat
    38461 gaactgaatc tctacatcga gggcaaccgt ctaccattgt cagagtataa cgctttgttt
    38521 gctgatggtg agtggggtta aaaatggctg ttcaatcata caaatacgaa tcccctttgg
    38581 ataagggata cattcgtatc aagttaagtc gttatcagca taaggctatt tttaaggtaa
    38641 ggaaaattcg catattggat gcgtatgcgt attactacaa cggtgaaaat gttgttgttg
    38701 aacactttct tgcacgatgg tttgtagctc tgatttttgt ccctctcctg ttaattggta
    38761 cttttgtgga tggttttcct agtacatggc gggagataaa gaaaggccta ttcccgcgta
    38821 agtacggcag attttcgcaa gatagctggc gagttatacc tggtaaacat tctgtagatg
    38881 agcaaccgat tgttgatttc tggttgaatc gtatggctga tgctaaaaaa gttgattgaa
    38941 ttgtgatgac tgttacagga gataaagtga agagttttat ttttgtttta tgttttgcct
    39001 tttctgctgc ggtttatgcc caggaagata gcatttctgt tattaactca tcagcggacc
    39061 ttgcatgtaa aaataatccc gatgctgcat caaaagagtc atgcaaagag ctgctccttt
    39121 cgacggctgc ttttgcttct gaaaatgcaa aattttattc agccttttgt cgcggctatg
    39181 ttaaggacga agataaagaa aaatgtgatg atgccggtaa gttagttagg tatttccaat
    39241 ctcttaatca ataatggcct ttgattaacg atcaacttct gatcactcgt tcaagcaaac
    39301 tataaaaaca cctgtccccg tgactggtca cggggtaaaa tgtctttgca aattattgac
    39361 gtgcgcgtcg ttttgacggt aaagtttact ccgctacggt aaatcccgta gccgggcgta
    39421 ggaacccgag taaatgaagc gcacaacacg cgccagcgtg tttttttgtg tgtaaaatct
    39481 gcgtacacca aatttatggt ggctcagatg gggcagcttt tagctggccg gtttcttcgt
    39541 ttaccggtat tcctaccccc gtctgggcta ccgccatata gagcgtagga actctggtgg
    39601 tagcacgacc aattaaacgg agtgcatacc atgttcaaat tcaaatttgc ggcaatcgtt
    39661 cgcacagaca aaaagtcaca catccatcat ctttccacca tcgcgtcatc tgaacgggaa
    39721 gctcgtcgcc agttcgccag ccggtttgtc ctggtattgt ctgcccgtat tccggtgcgc
    39781 gaggtggctg catgaatcag gtaaaaatga acactgttgg cctgctggaa tcgttggatg
    39841 aacgtctggc tcaggtctat gcgctcgtat catcagccca tcggacaatc tccagttgtg
    39901 aggcgtcgat ttatctctca gaagcaggga aactccttca aattgcgtgt gagctaacac
    39961 acgaagcaca gggttattca gcatcgctct cgtcgcaatt gctgcacgat aaggaggctg
    40021 aatgagcgcg ttagcagtct tttcctttca ggaagagcat caggttcgtg tagtgatgat
    40081 taacggcgaa ccgtggtttg ttgcatctga tgtgtgtatg gcggcgggta ttgattctac
    40141 ggccatcaga aagttggatg aggacgaaaa ggggcaggcg aggtagacca cacaacaagg
    40201 caccgataat tcggtgcctt taccgtatgc tttatccgaa atatgtcagc catgctaact
    40261 gcattgctga tatagggctg tttgcactgc cggaccaagc ataattctgg ccattataat
    40321 gaaactcaac aacgtatgag ccatcaccaa tatttctcac aggcttaaat accggcttag
    40381 atacaggtga agaaggcgcg ggcggttgct gttcttctac gctctcttcc tcttcctctt
    40441 cctctatctc aatttcgtcg tcatccagat cgtcatcctc tgataattca tccgtctgtt
    40501 caggtattgc ttccagctct tcttcgtcag gcataacgat ttctggagaa tctattttca
    40561 gctgccattg tccattttcg ccaacaaatt gccccagagc atcagcagca aattccaggt
    40621 atcgactaat cattgtaggg ctaaatttga atgctcttag agtgctgttc gtgattttta
    40681 tagaggggtc atgctctacc agctgcttaa ctgtgttatg cagacgaacc ccggcatctc
    40741 ctctggcgaa tcctggcatt tcgtcatcaa gctgctgcaa tgcttccagc ctggtatttt
    40801 cgtttccagt atttggcctc catgtctttg agaagttggc cagcttgaat tgcttatagt
    40861 gtaattgagt gttttcatcg tcatggccta aaatttccat aaagaaaaca tcctcatcta
    40921 cgtttttcca acgaggatct acgcggaaaa acatttcata cgcaatacgg gcataaatag
    40981 cacgactatc tttataaaca cggcggtcat ctttgaaaaa tgatttgacc cacgggttaa
    41041 aagcctttgc taatatggca ttaatccgtc cgttttcaga gcgtgtgtcg ttatctccat
    41101 aacctttaac gacatcatca aaatcagatg ctgctgggca tgagcgaagt acatttatta
    41161 aataaacaaa tgtttttgct tcgcataatg tatatatttt tctggttata tcatcagaag
    41221 ttcttttttt tgcttggcct aaaaaatcaa cggtatactt accagaaaca gtaaatgtac
    41281 cttgatacat aatctcaatc attcgtcttc ctgacaatgc agcaagtgca aatgccaaag
    41341 gagccatacc tgaacgagtg tttagattat ttaatgtagc agggctattt ataatatcgt
    41401 aaatagcttg catatattta gggtaatcaa tgaccaccac gttacgtttc ttattgtgca
    41461 gaacgtccgc ccatcgttgc tggatagatg cgcgttcggc agggcttagt tgaagatgat
    41521 agagaatctc atggttaact tttagctgat tcaactcttc aagtaaggca gatccttgct
    41581 gtagtaattt atataaataa tcgcgcttat ctttccagtt atcgctagtt aaatcagata
    41641 tggcgaattg ccactcttta tatttatttg ataattttaa taacttatta tgacttttgt
    41701 tatcaccaat tctgatatta gataaatctt ctgataaagg gagaattaat tttaattttg
    41761 cctgtaaagc tgacattctc tggcgaatat cggcagcagg tagagaaagc catgacaata
    41821 gctcctcatt atataaagga tacttttcag ataggcgatg tatatttttt tcaaagctat
    41881 gatgaagttt atcatcaaac cgttttctgg ccctgctcat ataggcattg aatgtgttgg
    41941 ctgtaattct tttttctaat cctttccccc taaactttcg cttatcatta aataatgcat
    42001 ttttaaattt taatgcaaca gcctttattt tttttgtttt ttccccttga ggacggtcag
    42061 agttatctat tgactcaaca tcgctaacaa gtgagtttat taattcaccg atttttactt
    42121 tgctcatctt tcctctcctg attatcagct ctgattatcg taacacaatt tgagaataag
    42181 ataactaata acgcgttaac ttagccacaa actaacagaa cataatgacg gataataaac
    42241 aaaaacacaa tatcggaacg ttaaaatttg ttccttgact ttgttctaaa aatcagcaca
    42301 taat
//
LOCUS       DAZPDU010000015        41242 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZPDU010000015 DAZPDU010000000
VERSION     DAZPDU010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41242
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.015"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..215)
                     /locus_tag="ACHURS_000739"
     CDS             complement(<1..215)
                     /locus_tag="ACHURS_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR1236550.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDT"
     gene            complement(286..2400)
                     /gene="fusA"
                     /locus_tag="ACHURS_000740"
     CDS             complement(286..2400)
                     /gene="fusA"
                     /locus_tag="ACHURS_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFR1236551.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2497..2967)
                     /gene="rpsG"
                     /locus_tag="ACHURS_000741"
     CDS             complement(2497..2967)
                     /gene="rpsG"
                     /locus_tag="ACHURS_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFR1236552.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3064..3438)
                     /gene="rpsL"
                     /locus_tag="ACHURS_000742"
     CDS             complement(3064..3438)
                     /gene="rpsL"
                     /locus_tag="ACHURS_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFR1236553.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3564..3851)
                     /gene="tusB"
                     /locus_tag="ACHURS_000743"
     CDS             complement(3564..3851)
                     /gene="tusB"
                     /locus_tag="ACHURS_000743"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFR1236554.1"
                     /translation="MLHTLHRSPWLADFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3859..4218)
                     /gene="tusC"
                     /locus_tag="ACHURS_000744"
     CDS             complement(3859..4218)
                     /gene="tusC"
                     /locus_tag="ACHURS_000744"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFR1236555.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4218..4604)
                     /gene="tusD"
                     /locus_tag="ACHURS_000745"
     CDS             complement(4218..4604)
                     /gene="tusD"
                     /locus_tag="ACHURS_000745"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFR1236556.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4604..5326)
                     /locus_tag="ACHURS_000746"
     CDS             complement(4604..5326)
                     /locus_tag="ACHURS_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1236557.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5493..6305)
                     /gene="fkpA"
                     /locus_tag="ACHURS_000747"
     CDS             complement(5493..6305)
                     /gene="fkpA"
                     /locus_tag="ACHURS_000747"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR1236558.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6526..6744
                     /locus_tag="ACHURS_000748"
     CDS             6526..6744
                     /locus_tag="ACHURS_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFR1236559.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6793..7383)
                     /gene="slyD"
                     /locus_tag="ACHURS_000749"
     CDS             complement(6793..7383)
                     /gene="slyD"
                     /locus_tag="ACHURS_000749"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFR1236560.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7478..7678)
                     /locus_tag="ACHURS_000750"
     CDS             complement(7478..7678)
                     /locus_tag="ACHURS_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFR1236561.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7688..9493)
                     /gene="kefB"
                     /locus_tag="ACHURS_000751"
     CDS             complement(7688..9493)
                     /gene="kefB"
                     /locus_tag="ACHURS_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFR1236562.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="ACHURS_000752"
     CDS             complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="ACHURS_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFR1236563.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10175..12088
                     /locus_tag="ACHURS_000753"
     CDS             10175..12088
                     /locus_tag="ACHURS_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1236564.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12088..13110
                     /locus_tag="ACHURS_000754"
     CDS             12088..13110
                     /locus_tag="ACHURS_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFR1236565.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13104..13322
                     /locus_tag="ACHURS_000755"
     CDS             13104..13322
                     /locus_tag="ACHURS_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFR1236566.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13376..14245
                     /locus_tag="ACHURS_000756"
     CDS             13376..14245
                     /locus_tag="ACHURS_000756"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFR1236567.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14300..14704)
                     /locus_tag="ACHURS_000757"
     CDS             complement(14300..14704)
                     /locus_tag="ACHURS_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFR1236568.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15006..15638
                     /gene="crp"
                     /locus_tag="ACHURS_000758"
     CDS             15006..15638
                     /gene="crp"
                     /locus_tag="ACHURS_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFR1236569.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGSR"
     gene            15689..17779
                     /locus_tag="ACHURS_000759"
     CDS             15689..17779
                     /locus_tag="ACHURS_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFR1236570.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDSPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17846..19066)
                     /gene="argD"
                     /locus_tag="ACHURS_000760"
                     /gene_synonym="dapC"
     CDS             complement(17846..19066)
                     /gene="argD"
                     /locus_tag="ACHURS_000760"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFR1236571.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGEILWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINPPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19152..19715)
                     /gene="pabA"
                     /locus_tag="ACHURS_000761"
     CDS             complement(19152..19715)
                     /gene="pabA"
                     /locus_tag="ACHURS_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFR1236572.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19747..20349)
                     /locus_tag="ACHURS_000762"
     CDS             complement(19747..20349)
                     /locus_tag="ACHURS_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFR1236573.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSKAGESE"
     gene            complement(20339..20506)
                     /locus_tag="ACHURS_000763"
     CDS             complement(20339..20506)
                     /locus_tag="ACHURS_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFR1236574.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20611..21183)
                     /gene="ppiA"
                     /locus_tag="ACHURS_000764"
                     /gene_synonym="rotA"
     CDS             complement(20611..21183)
                     /gene="ppiA"
                     /locus_tag="ACHURS_000764"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFR1236575.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21454..22635
                     /gene="tsgA"
                     /locus_tag="ACHURS_000765"
     CDS             21454..22635
                     /gene="tsgA"
                     /locus_tag="ACHURS_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFR1236576.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22897..25440
                     /gene="nirB"
                     /locus_tag="ACHURS_000766"
     CDS             22897..25440
                     /gene="nirB"
                     /locus_tag="ACHURS_000766"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFR1236577.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25437..25763
                     /gene="nirD"
                     /locus_tag="ACHURS_000767"
     CDS             25437..25763
                     /gene="nirD"
                     /locus_tag="ACHURS_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFR1236578.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25889..26695
                     /gene="nirC"
                     /locus_tag="ACHURS_000768"
     CDS             25889..26695
                     /gene="nirC"
                     /locus_tag="ACHURS_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFR1236579.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHAMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26714..28087
                     /gene="cysG"
                     /locus_tag="ACHURS_000769"
     CDS             26714..28087
                     /gene="cysG"
                     /locus_tag="ACHURS_000769"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFR1236580.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28334..28501
                     /locus_tag="ACHURS_000770"
     CDS             28334..28501
                     /locus_tag="ACHURS_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFR1236581.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28796..30133
                     /gene="frlA"
                     /locus_tag="ACHURS_000771"
     CDS             28796..30133
                     /gene="frlA"
                     /locus_tag="ACHURS_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HFR1236582.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSI
                     ILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGL
                     MTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            30154..31176
                     /gene="frlB"
                     /locus_tag="ACHURS_000772"
     CDS             30154..31176
                     /gene="frlB"
                     /locus_tag="ACHURS_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFR1236583.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKPLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31226..32057
                     /gene="frlC"
                     /locus_tag="ACHURS_000773"
                     /pseudo
     CDS             31226..32057
                     /gene="frlC"
                     /locus_tag="ACHURS_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
     gene            32054..32839
                     /gene="frlD"
                     /locus_tag="ACHURS_000774"
     CDS             32054..32839
                     /gene="frlD"
                     /locus_tag="ACHURS_000774"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFR1236584.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TLVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            32939..33670
                     /gene="frlR"
                     /locus_tag="ACHURS_000775"
                     /gene_synonym="yhfR"
     CDS             32939..33670
                     /gene="frlR"
                     /locus_tag="ACHURS_000775"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFR1236585.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33822..34907)
                     /locus_tag="ACHURS_000776"
     CDS             complement(33822..34907)
                     /locus_tag="ACHURS_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFR1236586.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAALAALLKPGQRLLVHDAPVYP
                     TTQVIIEQMGLTLITADFNDLSALKQVVDEQLPDAALVQHTRQQPQDGYILADVLATL
                     RSAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRS
                     TLYSGGSQIQGAQALEVLCGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRNGEETVLRILRESIASI"
     gene            complement(34919..36223)
                     /locus_tag="ACHURS_000777"
     CDS             complement(34919..36223)
                     /locus_tag="ACHURS_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFR1236587.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPIVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36235..36588)
                     /locus_tag="ACHURS_000778"
     CDS             complement(36235..36588)
                     /locus_tag="ACHURS_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFR1236588.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36599..37477)
                     /locus_tag="ACHURS_000779"
     CDS             complement(36599..37477)
                     /locus_tag="ACHURS_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFR1236589.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLGVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            complement(37474..38700)
                     /locus_tag="ACHURS_000780"
                     /pseudo
     CDS             complement(37474..38700)
                     /locus_tag="ACHURS_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
     gene            complement(38700..39863)
                     /locus_tag="ACHURS_000781"
                     /pseudo
     CDS             complement(38700..39863)
                     /locus_tag="ACHURS_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
     gene            complement(39947..40309)
                     /locus_tag="ACHURS_000782"
     CDS             complement(39947..40309)
                     /locus_tag="ACHURS_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFR1236590.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(40326..41231)
                     /gene="yhfZ"
                     /locus_tag="ACHURS_000783"
     CDS             complement(40326..41231)
                     /gene="yhfZ"
                     /locus_tag="ACHURS_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFR1236591.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
BASE COUNT         9916 a        11248 c        10372 g         9706 t
ORIGIN      
        1 gtgtcatatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
       61 ttatcgatct ggtcgaatgc acgagcagca ccgccgtagg ttttagccag tacggtggtg
      121 attgcagcgg tcagagtagt tttaccgtgg tcaacgtggc cgatagtacc aacgttaacg
      181 tgcggttttg tacgttcaaa tttttcttta gacacggcta tattccttac tatagtgctc
      241 tccccttcag gagagagcac gggactttgg tattaaccct taggcttatt taccacgggc
      301 ttcaattacg gcctgagcaa cgttactcgg cgcttcatca tacttcagga attccatagt
      361 gtatgatgca cgacctttgg tcagagaacg cagctgagtt gcgtatccga acatttcaga
      421 cagcggtact tcagcgtgga tcttaacgcc agtaacttca gattcctgac ctttgagcat
      481 accacgacga cggctcaagt caccgataac gtcaccggtg ttctcttccg gagtttctac
      541 ttcaaccttc atgatcggct caagcagaac tggtttcgct ttcttaaagc cttctttaaa
      601 ggcgatagaa gcagccagtt taaacgccag ttcagaggag tcaacgtcat ggtaagaacc
      661 gaagtgcaga cgaataccca tgtctactac cgggtagcct gccagcggac ctgctttcag
      721 ctgttcctgg atacctttat caacggccgg gatgtattcg ccagggatta caccaccttt
      781 aatgtcgttg atgaactcgt agcctttcgg gtttgaaccc ggctccagcg ggtacatgtc
      841 gataacaaca tgaccatact gaccacgacc accagactgt ttcgcgtgtt taccttcaac
      901 atcggtaact ttctggcgga tagtttcacg gtaagcaacc tgcggtttac ctacgttcgc
      961 ttcaacgttg aattcacgct tcatacggtc aacgatgatg tcgaggtgca gttcgcccat
     1021 acccgcgatg atggtctggt tagattcttc gtcagtccat acacggaaag acgggtcttc
     1081 tttagccaga cggcccagag ccagacccat tttttcctgg tcagctttgg ttttcggttc
     1141 aactgcgatg gagattaccg gctcagggaa ttccatacgt tccagaatga tcggcgcatc
     1201 cgggtcacac agggtgtcac cagtggttac gtctttcaga ccgatagcag cagcgatgtc
     1261 gcccgcgcga acttctttga tctcttcacg tttgttagcg tgcatctgaa cgatacgacc
     1321 gaaacgctca cgtgcagctt tcacggagtt cagtacggta tcaccagagt taaccacacc
     1381 ggagtaaaca cggaagaagg tcaggttacc aacaaacggg tcggtagcga ttttgaacgc
     1441 cagtgcagag aacggctcgt catcacttgc gtgacgctca gccggagtgt ctttaccgtc
     1501 gtccaggata ccgttgatcg caggtacgtc aaccggggat ggcaggtaat caattaccgc
     1561 atccagcatc gcctgaacac ctttgttctt gaacgcagaa ccacaggtta ccaggatgat
     1621 ttcgttgttc agaacgcgct gacgcagagc acctttgatt tctgcttcag tcagttcttc
     1681 accacccagg tatttttcca tcagctcttc agaagcttca gctgcggatt cgatcaggtt
     1741 ctggtgccat tcgttagcca gttcaaccat gtctgccggg atatcttcgt attcgaaggt
     1801 tacgccctgg tcagcgtcgt tccagttgat agctttcatt ttcaccaggt caacaacacc
     1861 ggtgaaatgt tcttcagcac caatcgccag ctgcagcgga accgggttcg cgcccagacg
     1921 ggttttgatc tggttaacaa ctttcaggaa gttcgcaccc atgcggtcca ttttgttaac
     1981 gaacgcaatg cgcggaactt tatatttgtt tgcctgacgc catacggttt cagactgcgg
     2041 ctgaacacca ccaactgcgc agtaaaccat taccgcacca tcgagaacac gcatggaacg
     2101 ttctacttcg attgtgaagt caacgtgccc cggggtgtcg atgatgttga tgcgatgcgg
     2161 ctcatactgc ttagccatac cagaccagaa tgcagtagta gcagcggaag tgatggtaat
     2221 accacgttcc tgctcctgct ccatccagtc catggttgca gcgccgtcat gaacttcacc
     2281 gattttatgg tttacaccgg tgtagaacag aatacgttcg gtagtagtgg ttttaccggc
     2341 gtcgatgtgc gcactgatac cgatgttacg gtagcgtgcg atgggtgttg tacgagccat
     2401 ttgtttcctc gtttatcttt taggcgttca atttaagtag cccaaagcgg gctgcttact
     2461 ggaagcgccc gcctggtgac taaaactccg aagggattac caacggtagt gtgcgaacgc
     2521 cttgttggct tcggccatac ggtgaacgtc ttcacgtttc ttaactgcag tacctttgtt
     2581 ttctgcagca tcagaaagtt cgttcgccag gcgcagagcc atggatttat caccgcgttt
     2641 acgagcagct tcaacgatcc aacgcattgc cagagcatta cgacgaaccg gacggacttc
     2701 aactggtacc tgataagtag aaccaccaac gcggcgagac ttaacttcta cagtcgggcg
     2761 cacgttttcg agagctactt cgaatgcttc cagttcagat ttaccagagc gctgagccag
     2821 ggtctccagc gcgctgtata cgatagattc agcagtagat tttttaccat ctaccatcag
     2881 gatatttaca aatttagcca gcagttctga tccgaacttc ggatccggca gaattttacg
     2941 ctgaccaatg acgcgacgac gtggcatgga aatactccgt tgttaattca ggattgtcca
     3001 aaactctacg agtttagttt gacatttaag ttaaaacgtt tggccttact taacggagaa
     3061 ccattaagcc ttaggacgct tcacgccata cttggaacga gcctgcttac ggtctttaac
     3121 gccggagcag tcaagcgcac cacgtacggt gtggtaacga acacccggga ggtctttaac
     3181 acgaccgcca cggatcagga tcacggagtg ctcctgcagg ttgtgacctt caccaccgat
     3241 gtaggaagtc acttcgaaac cgttagtcag acgaacacgg catactttac gcagcgcgga
     3301 gttcggtttt ttaggagtgg tagtatatac acgagtacat acgccacgtt tttgcgggca
     3361 tgcttccagc gcaggcacgt tgcttttcgc aactttgcga gcacgtggtt tgcgtaccag
     3421 ctggttaact gttgccatta aatagctcct ggttttagct tttgcttcgt aaacacgtaa
     3481 taaaacgtcc tcacacaata tgaggacgcc gaattttagg gcgatgccga aaagatgtca
     3541 agaaatatac aacgatcccg ccatcaccag gccatctggc tggagtgctt aaccgtaagt
     3601 ctgacgaaat cagtatagtc aatgggaatg atgtcgttcg aaatttgacc agtcaaaccg
     3661 cgggcaataa ggtcttcgtt cagggcatag accttaatgg gggcattacg cagactttca
     3721 aggtagcggt taccgtcaac tgcggcagtt acgccatctt gcaataatag cagttcgtcg
     3781 ccttcactga gcagacgcag cagcgcagca aaatccgcca gccagggtga acgatgtaat
     3841 gtgtgcagca taaaagcctc aaaacctcaa aataacatcg tagttggcga gttcgcggcg
     3901 taaggcatct gcttcgagcg gcgtggcttc gacaacaaag ggggtctgcg gatctaaccc
     3961 gcgttcgcgc agtgaagccg cacaaaccca gcactgttca atgtcgtaca gacccaacaa
     4021 tttaaaagtg gcaatgtaat cacgcgccag cactgcatcg ggcttttgtc ctggcagcag
     4081 ctgaaaaacg ccatcagcta taaagaagac agccagatca tcagttaatg cggaagttgc
     4141 cagtaaagca tctaaacctt cccgaccagc agctgtacca tgaggcgcag tagaaaaaac
     4201 aaacgcaatt cgtttcatca gaactgtacc accctgtcac aggtcagcga ggcttccgcc
     4261 agcgcgccaa gtccgcttaa ggtaaatccc ggctgaaggt ttgacgaggc cagccccagc
     4321 cttccggcct ccgtttcatc aacaacgcca cggcgtaatg ctgccgctac gcagatattc
     4381 agcgccacac catgttgcgc attcaactgt tgccagccgc gcacaaggtc aaattcgtcg
     4441 cttgccggtg aggtcaattg gttagcgtta tagacccctt cccgataaaa aaagacgctg
     4501 cttaactcat ggccttctac tatcagcgcc tgcgcaaact gaaaagcact actcgcctgt
     4561 tgcgtaccgt atgctggccc ggtcaccacg atggcaaaac gcattactta tcttgcccct
     4621 ggaaatcacc gcttttgaac tggcggatgt agagatagac agtgtgtttg gagatgttca
     4681 ggcggtcagc aacctggttg atcgcgtctt taatatcgaa gatccctttc tcgtagagat
     4741 tcagcacgat ctgacgattt ttggcgttat tagaaacatt gcgatcggca tttacttctt
     4801 cgatggtgaa ctccagcgtt tgggtaacca gatcttcaac agaagaggca aagttgacgc
     4861 ttgaaccgac atccggggtt tctggcggca caaaagtgct cataatctgc gagaagggaa
     4921 catcaagatt catattgatg cacagcagac caattacacg ctgttcgcgg ttacgaatcg
     4981 cgatagtcag ggacttcatt aatacgccgc ttttggcgcg agtaaagtag catttagaaa
     5041 cgctgctatc cgctcccgtc atatcgtgca gcatacgtag cgccaggtca gtaattggcg
     5101 aaccaatctt ccggcctgta tgttcaccgt tagcaatgcg aatggctgaa cattttagat
     5161 cctgcaaaga gtgcaaaacg atttcacagt gggagccaat aagcatcgct aacccgtcca
     5221 ccaccgcttc gtaggatttc agaatatcaa aatcggtctg gtcgaaagga cgttgatcca
     5281 gtaaatccaa ctcactggtt tcgttggtta aaagcgacct ggacatgaaa aaaaacactc
     5341 cttttcagga gcctgtcgtt aacttttcag ggcaggctca ttaatgatgc gggtaactaa
     5401 attaatacag cggaggttcc gctttccagc actaattata tccggcctgt aataaaaaaa
     5461 ccgccgcctg gtcaggcggc ggttcttaat gcttattttt tagcagaatc tgcggctttc
     5521 gcatcagctt ccggctttgc atcagccttc ggcgctggtt tcacatccag cagctctacg
     5581 tcaaacacca gggtagaatt cggtgggatc cccggaacac ccgctttgcc gtaagccagt
     5641 tctggtggaa taaccagttt gatcttaccg cctttcttga tgttcttcag accttctgtc
     5701 caccccggga taacaccgtc cagacggaaa gagagcggtt caccacgggt gtaagagttg
     5761 tcgaactctt taccgtcgat cagcgtacct ttgtagttca ctacaacagt atcgctgtct
     5821 ttcggtgctt cgcctttacc ggcttctact acctgataaa ccagaccagt tgaagaggtt
     5881 ttcacacctt tctctttggc aaatttctcg cggtactctt taccttttgc ttcgttatca
     5941 gccgcgtctt tttccatctt cgcctgagca gaagacttca cgcgagcttc gaatgcttgc
     6001 agagtctgtt cgatctcttg gtcggagagt ttgctcttat cagcaaatgc atcctgaaca
     6061 ccagcgatca gctgatcttt atccagtttg atgcccagtt tttcttgttc tttaagagag
     6121 ttttccatgt aacgacccag cgaagcaccc agtgcataag ctgatttctg atcgtcattt
     6181 ttgaacgctg ctttgctgtc agcagttgta gcaggttttg cagcttcagc agcaaaagtg
     6241 attggtgcat gcagggcaac ggccattgtg gtcgccagca gcgttacttt aaacagtgat
     6301 ttcatccata tctccaggat cggggcatct caccccaggg ttaactatta tcagaagggt
     6361 actataaagc gttgtcgaac aaatctacat acagacacgc cctattatca tctattttca
     6421 gactcttttt gtttaaatta gtttcgatga ccgcgaaatg agtgctgtct cgggcagcaa
     6481 agttaagtag aatccgcggc gaccattcga caaaagaggt gaatcatgca ggatttatca
     6541 ttggaagcac gcctggcaga gctggagagc cgactggctt ttcaggaaat caccattgaa
     6601 gaactgaacg tcacggtgac cgctcatgaa atggagatgg cgaaactgcg cgatcatctg
     6661 cgtctgctga ccgagaagtt aaaagccagc cagccgtcga acatcgcgtc gcaggctgaa
     6721 gaaacgccac cgccacatta ttgaggcgta aaaaaagcgg ggattccccg cttttttgtc
     6781 accttttcgg tattagtggc aaccacaacc gccgttgcct ttaccgccac agcagccttc
     6841 gccaccgtgt tcatgaccgt gatcgtggcc atgaccgccg cagcaaccgt cgtggtcgtg
     6901 atcgtggtga tgatcgtgcg cgccgtgaac gtgaccatga gccagttctt cttcggtcgc
     6961 ttcacgaatc gccacaacct caacgttgaa tttcaggttc tgaccggcca gcatgtggtt
     7021 accatcaacc actacgtgat cgtcttcaac cgcagtgatt tcaaccggta ccggaccctg
     7081 gtcggtttca gccaggaaac gcatacctac ctgcagttca tcaacgccca taaatacgtc
     7141 tttaggaaca cgttgcacca ggttttcgtc gtactgaccg taagcgtcgt tcgcgccaac
     7201 agcgacatca aatttgtcgc caacttcatg accttccagc gccgtttcca ggccagagat
     7261 cagggaaccg tgaccatgca ggtagtccag cggcgcactc accggagact catcaaccaa
     7321 cacaccgtct tctgtacgta cctgataggc caggctgacc accaggtctt ttgctacttt
     7381 catgatatct cctgagcatg ggaagaatag tggcgcagat tgtagcggaa ttctgcagcc
     7441 gtgtactcac tagcttaaaa aaactcggcg catatcgcta gtccggatga aaaatcccga
     7501 tcacttgctc atctttgcga acgtgatcgc gggcttcttt gtctgcttct cgcatctgat
     7561 gtccgcactt aacgcattca acaatatcaa tattattttc gcgccacatc gccattgaat
     7621 cctgtgcctg acaggccggg cattttgcgc ccgcaataaa acgttttcgg attgccatct
     7681 ttaccctcta ctcaaattca tcccagccgt ccagctggcg tcgttcttgt tgcatttcac
     7741 gctggaaaat ctcttccagt tcgcgtcggg cttccctggc gcgagaaatt tgtacagtat
     7801 cggcatgcat tgggatgagc tctcgcagca tccgcatatc caggcggcga aaatgcagtt
     7861 gcgcccgctg cgcctgatgc ggatgcatcc caagcgtgac caaagtcttg cgccccagct
     7921 ctaacgcact ggagaatgtt tcacgggaaa actgcgtcac ccctgcctgt aataactcat
     7981 gcgcttccac acgtccgcgc gctcgcgcaa gaatatgcaa atgcggaaag tgctgctggc
     8041 atatttccac cagcttcatg gtgtcttccg gctcgttaca ggtaatgacg atagactcag
     8101 cggcctctgc acccgcagaa cgtaatagat cgacctgcgt ggcgtcgccg taataaactt
     8161 tgtagccata tttacgcatc aggttaacgg cgctgatatc ccgctccagc acggtaatgc
     8221 gcattttatt tgccatcagc aaacgaccaa tcacctgacc aaaacgcccg aagcccacga
     8281 caatgacctg gggtttatca tcgttgaccc acggtttttc gtcttcttct tccggtccgt
     8341 taaactggcg ggatagccat ttatccacca gcttcatcag caacggcgtg gtcatcatgg
     8401 aaagcgtcac cgtcaccagc aacagcgcca tctggtcgcc ctggaataaa cgttgtgaag
     8461 aagcggtaga aaagaggaca aaggcaaact caccgccctg actcaacacg ccagcaaact
     8521 gcatccgctc tgagctacgc acgccataca atcgcgccag cagatacagc acgagaattt
     8581 tcaccgccac cagcacaacc acgcttatca ctacccacaa cagatgggta taaagcaccc
     8641 cgaggttgag cgacatgccg acagagataa agaacaaacc gagcagcaag cctttgaagg
     8701 gatcgatagc cgtttccagt tcatggcgat attcactttc cgccagcagc acgcccgcaa
     8761 taaatgtacc gagcgccatc gacagcccca gcgcatccat aaacaatgcg gaacccaaca
     8821 ccagcagcag cgtcgcggcg gtgaacactt cccgcacgcc agaagctgca ataaagcgga
     8881 ataccggacg cagtaaatag cgcccaccaa tcagcatgcc gacaaacgcc agcaccttca
     8941 tgccgatctt catccagtcg aaatgttcgt ctgccgaccc cgccaacaac ggcactaacg
     9001 ccagtgctgg gatcactgcc agatcctgaa acagcagaac cgaaaacccg agctggccgg
     9061 attcgctgcg attcatccct ttctcacgca tcaattgcaa cgccattgca gttgaagaca
     9121 tcgcaaggcc aatgccaccg accaccgccg cctgccaggc gaaatccgtc agcatcaata
     9181 atcccgccag caacgccgcg cttaacagca cctgtgccgc gcctacgcca aaaatcgaac
     9241 gccgcagttg ccaaagtttg gagggattca actcaaggcc gatgataaac atcaggaata
     9301 ccacgccgag ttccgaaaag tggaggatct catcgacgtc gctaataaac cccagccccc
     9361 acgggccaat tgcaatccct gccagcaaat atcccaacac agcgccaatc cccagccgcg
     9421 atgccaacgg caccgcagcc accgccgcga agagaaacag cactcctgcg agtaaaaaat
     9481 cggaaccttc catcagcggc ctcctggaga cagcggattt gccagccagt caccgtaggc
     9541 tctggcgtgg ctcgccagct cctttgcgct ttgccgtctc gcccagtaaa taatgatggg
     9601 acttaaccaa tgcatccggc acatgcccgc cgccagttca aaggggcgca gcacatcgct
     9661 catcgggtag cgattcagcg cgtcataacg gtaagcactt tccggctcgc cggtggtaat
     9721 cacgttacgc cagtactttc ccgccagttg gtttcctccc ggcccgctgg caaaaccacg
     9781 acttaatacc cggtccagcc actctttcag tagcgccggg cagctatagg tataaagagg
     9841 atgctgaaag acaatcacct cgtgctcgcg cagtaatgcc tgctcacggg ggatatcaat
     9901 aaaaaaatcg ggatagtgcg cgtaaaggtc gtgcacggta acattgctga gctgcgtggc
     9961 cggtttaagc agtacccggt ttgccaccga gtcctgagat tccggatggg catacagcag
    10021 caaaactttc gctggctgag acatcatccc cctcccggta tggtttttgt gtatagtcgc
    10081 tgttttgggc taccattgcg cccggtgcgg cagctcgccc atacattaca ttatcataat
    10141 gataagttaa catagtctga acatacggcg ccttatgatt gttttctcct cgttacaaat
    10201 tcgtcgcggc gtgcgcgtcc tgctggataa tgccaccgcc accatcaacc ccgggcagaa
    10261 agtcggcctg gtgggtaaaa acggctgtgg taaatctacc ctgctggcat tgctgaaaaa
    10321 tgaaatcagc gccgacggcg gcagctacac ctttccggga agctggcaac tggcgtgggt
    10381 gaatcaggaa acgccagcgt taccgcaagc ggcgctggaa tatgtcattg acggcgaccg
    10441 tgaatatcgt caactggaag cgcagctgca cgacgccaac gaacgtaacg acgggcacgc
    10501 cattgcgacc attcatggca agctggatgc tattgacgca tggagtattc gctcccgtgc
    10561 cgccagcctg ctgcacggcc tcggtttcag caatgaacaa ctggagcgcc cggtaagtga
    10621 tttctccggt ggctggcgta tgcgtcttaa ccttgcccag gcgctgattt gccgttcaga
    10681 cttgctgctg ctcgacgaac cgactaacca cctcgatctc gatgccgtta tctggctgga
    10741 aaaatggttg aagagctatc agggcacgct gatcctgatc tctcacgacc gcgacttcct
    10801 cgatccgatc gttgataaaa ttattcatat cgaacaacaa agcatgttcg agtacaccgg
    10861 caactacagt tcgtttgaag tacagcgcgc cacccgtctg gcgcagcaac aagcgatgta
    10921 tgaaagccag caggaacgcg tggcacatct gcaaagttat atcgaccgtt tccgtgccaa
    10981 agccaccaaa gcgaagcagg cccagagccg cattaagatg cttgagcgta tggagctgat
    11041 tgcccccgcg cacgtcgaca acccgttccg ctttagcttc cgcgcgccgg aaagcctgcc
    11101 aaatccgtta ctgaagatgg aaaaagtcag cgcgggctat ggcgatcgca ttattctcga
    11161 ctcgattaaa ctgaacctgg tgcccggctc gcgcattggt ctgttaggcc gcaacggcgc
    11221 gggtaaatcg acattaatca aactgttagc cggtgaactt gcgccagtca gcggtgaaat
    11281 tggtctggcg aaagggatca agctcggcta cttcgcccag catcaacttg aatacctgcg
    11341 cgccgacgaa tcacctattc aacatctggc acgtttagcg ccgcaggagc tggaacaaaa
    11401 actgcgtgac tacctcggcg gctttggttt ccagggcgat aaagtaaccg aagaaacgcg
    11461 ccgcttctcc ggtggggaaa aagcccgcct ggtgctggca ttaattgtct ggcagcgtcc
    11521 gaatctgctg ctgctcgacg aaccgactaa ccaccttgac ctcgacatgc gtcaggcact
    11581 caccgaagca ttaatcgagt ttgaaggcgc gctggttgtc gtttcgcacg accgtcattt
    11641 gctgcgttcc accactgacg atctctacct ggttcacgat cgtaaagtcg aaccgttcga
    11701 cggcgatctg gaagattatc aacagtggtt gagcgacgta caaaagcagg aaaaccagac
    11761 cgacgaagcg ccaaaagaga acgcgaacag cgcccaggca cgtaaagatc agaagcgtcg
    11821 ggaagcggag ctgcgtgcgc aaacccagcc gctgcgtaaa gagattgccc gtctggaaaa
    11881 agagatggag aagctgaacg cgcaactggc gcaggcggaa gagaaactcg gagacagcga
    11941 actgtatgac cagagccgta aagcggagtt gaccgcctgc ctgcaacagc aagccagcgc
    12001 caaatccggc ctggaagagt gcgaaatggc gtggctggaa gcccaggagc agcttgagca
    12061 gatgctgctg gaaggccaaa gcaactgatg gcgcagataa cgacgaccga tgccaatgaa
    12121 ttcagcagca gtgctgaatt cacccctatg cgcggcttta gcaattgtca tctacaaact
    12181 atgctgccgc gtttgtttcg tcgtcaggtg aaattcaccc cgtactggca gcggctggag
    12241 ttgcccgacg gcgattttgt cgatctcgcg tggagtgaag cccctgcaca ggctcggcat
    12301 aaaccgcgtt tagtggtatt tcacgggctg gaaggcagtc tcaatagccc ttacgcccac
    12361 ggtctggttg aggcggcgca aaagcgcggc tggctgggcg tggtgatgca ttttcgcgga
    12421 tgcagcggtg aaccaaaccg tatgcaccgc atttaccatt cgggcgaaac cgaagacgcc
    12481 agttggtttt tacgctggct gcaacgcgaa tttggtcatg cgccaacggc tgccgtcggc
    12541 tattcgctcg gcggtaatat gctggcctgt ttgctggcaa aggaaggcaa tgatctcccg
    12601 gttgatgcgg cggtgattgt ctctgcgccg tttatgctgg aagcctgtag ctatcatatg
    12661 gaaaagggct tttcccgcgt ttatcagcgt tacttgctga acctgttaaa agccaatgcc
    12721 gcgcgcaagc tggcagccta ccccggaacg ctgccgatta atctcgcgca gttaaaatcg
    12781 gtacgtcgca tccgtgaatt tgacgatcta atcaccgcca gaattcacgg ctacgccgac
    12841 gctatcgact attatcgtca gtgtagcgcc atgccgatgc tgaaccggat cgccaaaccg
    12901 acgctgatta ttcacgccaa agacgatccg tttatggatc atcaggtgat cccgaaaccg
    12961 gaaagtctcc ccccgcaggt ggagtatcaa ctgactgaac atggcggtca tgttggcttt
    13021 attggcggta cattacttca tccgcaaatg tggctggagt cacgcattcc tgactggtta
    13081 acaacgtatc tggaggcgaa atcatgttga ttccgtggca agacctctcc cccgaaacgc
    13141 tggaaaattt aattgaaagc tttgtgttac gtgaaggcac cgattatggt gaacatgagc
    13201 gtacacttga acagaaagtc gccgacgtca aacgccagct acagtgcgga gaagcggtgc
    13261 tggtatggtc ggagctgcac gaaacggtca atatcatgcc gcgcagtcag tttcgcgaat
    13321 agcctgcaac cgtcggcgga cgagataata atgatagcta agtcatggag ttactatgtc
    13381 tgccaaacat ccggtcattg cggtaacagg atccagcggc gcggggacca ccaccaccag
    13441 cctcgcgttt cgtaaaatat tcgcgcagtt aaatctgcat gcagctgagg tggaaggcga
    13501 cagttttcac cgttacaccc gcccggaaat ggacatggcg atccgcaaag cgcgcgacgc
    13561 cgggcggcat atcagctact tcggccccga ggctaacgac ttcggcctgc tggaacaaac
    13621 cttcattgaa tacggtcaga gcggcaaagg gaaatctcgc aaatatctgc atacctacga
    13681 cgaagccgta ccgtggaatc aggtaccggg gacattcacc ccctggcaac ctttaccgga
    13741 acccactgat gtactgtttt atgaaggttt acacggcggc gtagtcacgc cacagcataa
    13801 cgttgcgcag catgtggact tactggtcgg cgtggtgcct atcgttaacc ttgagtggat
    13861 tcaaaaactg atccgcgaca ccagcgagcg cgggcactca cgagaagcag tgatggactc
    13921 agtagtgcgt tcaatggaag actatatcaa ctacatcaca ccgcagtttt cccgcaccca
    13981 tcttaacttc cagcgcgttc ccaccgtcga cacttcaaac ccgttcgcgg caaaaggtat
    14041 cccgtcgctc gatgaaagct ttgtggtgat ccattttcgt aatctggaag ggatcgattt
    14101 cccctggctg ctggcgatgt tgcaaggctc attcatttcc cacatcaata cgttagtggt
    14161 accgggcggc aaaatgggtc tggcaatgga attaattatg ctgccgctgg tgcaacgatt
    14221 gatggaagga aagaaaatcg agtaactctg ctattacgcc ggataaaata ctatccggct
    14281 tcacaacggg atagttaaat cacgcggcaa ccacttcata cgagtgagta atattcaccg
    14341 ctttttccag catcaacgcc actgaacaat atttctcagc agagagatca accgcacgcg
    14401 caaccgctgc gtctttcagg tcgcgaccgg tgacgataaa atgcagatta atgtgcgtaa
    14461 acaggcgtgg tgcctcttcg cggcgttcag aggtcaattt tacttcacaa tcgaccacat
    14521 cctgacgccc tttttgcagg atagaaacca catcgatggc actgcaacca cccgccgcca
    14581 tcaacaccat ttccatcgga cttggtgctt tatcgcctga gttgccgtcc attaaaatct
    14641 ggtgaccaga ggcggattcg cccagaaaag ttaacccttc gacccacttc actcgcgctt
    14701 gcatttttgc tactccactg cgtcaatttt cctgacagag tacgcgtact aaccaaatcg
    14761 cgcaacggaa ggcaacctgg gtcatgctga agcgagacac caggagacac aaagcgaaag
    14821 ctatgctaaa acagtcagga tgctacagta atacattgat gtactgcatg tatgcaaagg
    14881 acgtcacatt accgtgcagt acagttgata gccccttccc aggtagcggg aagcatattt
    14941 cggcaatcca gagacagcgg cgttatctgg ctctggagaa agcttataac agaggataac
    15001 cgcgcatggt gcttggcaaa ccgcaaacag acccgactct cgaatggttc ttgtctcatt
    15061 gccacattca taagtaccca tccaagagca cgcttattca ccagggtgaa aaagcggaaa
    15121 cgctgtacta catcgttaaa ggctctgtgg cagtgctgat caaagacgaa gagggtaaag
    15181 aaatgatcct ctcctatctg aatcagggtg attttattgg cgaactgggc ctgtttgaag
    15241 agggccagga acgtagcgca tgggtacgtg cgaaaaccgc ctgtgaagtg gctgaaattt
    15301 cgtacaaaaa atttcgccaa ttgattcagg taaacccgga cattctgatg cgtctgtctg
    15361 cacagatggc gcgtcgtctg caagtcactt cagagaaagt gggcaacctg gcgttcctcg
    15421 acgtgacggg ccgcattgca cagactctgc tgaatctggc aaaacaacca gacgctatga
    15481 ctcacccgga cggtatgcaa atcaaaatta cccgtcagga aatcggtcag attgtcggct
    15541 gttctcgtga aaccgtggga cgcattctga agatgctgga agatcagaac ctgatctccg
    15601 cacacggtaa aaccatcgtc gtttacggct ctcgttaatc ccgtcggagt ggcgcgttac
    15661 ctggtagcgc gccattttgt ttcccccgat gtggcgcaga ctgatttatc accccgatat
    15721 caactatgca cttcgacaaa cgctggtgct atgtttgccc gtggccgttg ggttaatgct
    15781 tggcgaatta cgattcggtc tgctcttctc cctcgttcct gcctgttgca atattgcggg
    15841 ccttgatacg cctcataaac gttttttcaa acgcttaatc attggtgcgt cgctgtttgc
    15901 cacctgtagc ttgctgacac agctactact ggcaaaagat gttcccctgc cctttttgct
    15961 gaccggatta acgctggtac ttggcgtcac tgctgagctg gggccattgc acgcaaaatt
    16021 gcttcctgca tcgctgctcg ccgccatttt taccctcagt ctggcgggat acatgccggt
    16081 ctgggaaccg ttgctcatct atgcgttggg cactctctgg tacggattgt ttaactggtt
    16141 ttggttctgg atctggcgcg aacaaccgct gcgcgagtca ctaagtctgc tgtaccgcga
    16201 actggcagat tattgtgaag ccaaatacag cctgcttacc cagcacaccg accctgaaaa
    16261 agcgctgccg ccgctgctgg tgcgccagca aaaagcggtc gatctaatta cccagtgcta
    16321 tcagcaaatg catatgcttt ccgcgcaaaa taatactgat tacaagcgga tgctgcgtat
    16381 tttccaggag gcgctggatt tacaggaaca tatttcggtc agtttgcatc agccggaaga
    16441 ggtgcaaaag ctggtcgagc gtagccatgc ggaagaagtt atccgctgga atgcgcaaac
    16501 cgtcgccgct cgcctgcgcg tgctggctga tgacattctt taccatcgcc tgccaacgcg
    16561 ttttacgatg gaaaagcaaa ttggcgcact ggaaaaaatc gcccgccagc atccggataa
    16621 tccggttggg caattctgct actggcattt cagccgcatc gcccgcgtgc tgcgcaccca
    16681 aaaaccgctc tatgcccgtg acttactggc cgataaacag cggcgaatgc cgttacttcc
    16741 ggcgctgaaa agttatctgt cactaaagtc tccggcgcta cgcaatgccg gacgactcag
    16801 tgtgatgtta agcgttgcca gcctgatggg caccgcgctg catctgccga agtcgtactg
    16861 gatcctgatg acggtattgc tggtgacaca aaatggctat ggcgcaaccc gtctgaggat
    16921 tgtgaatcgc tccgtgggaa ccgtggtcgg gttaatcatt gcgggcgtgg cgctgcactt
    16981 taaaattccc gaaggttaca ccctgacgtt gatgctgatt accaccctcg ccagctacct
    17041 gatattgcgc aaaaactacg gctgggcgac ggtcggtttt actattaccg cagtgtatac
    17101 cctgcaacta ttgtggttga acggcgagca atacatcctt ccgcgtctta tcgataccat
    17161 tattggttgt ttaattgctt tcggcggtac tgtctggctg tggccgcagt ggcagagcgg
    17221 gttattgcgt aaaaacgccc atgacgcttt agaagcctat caggaagcga ttcgcttgat
    17281 tcttagcgag gatccgcaac ctacgccact ggcctggcag cgaatgcggg taaatcaggc
    17341 acataacact ctgtataact cattgaatca ggcgatgcag gaaccggcgt ttaacagcca
    17401 ttatctggca gatatgaaac tgtgggtaac gcacagccag tttattgttg agcatattaa
    17461 tgccatgacc acgctggcgc gggaacaccg ggcattgcca cctgaactgg cacaagagta
    17521 tttacagtct tgtgaaatcg ccattcagcg ttgtcagcag cgactggaat atgacgaacc
    17581 ggggagttct ggcgatgcca atatcatgga ttcgccggag atgcagccgc acgaaggcgc
    17641 ggcaggtacg ctggagcagc atttacagcg ggttattggt catctgaaca ccatgcacac
    17701 catttcgtcg atggcatggc gtcagcgacc gcatcacggg atttggctga gtcgcaagtt
    17761 gcgggattcg aaggcgtaat gcaggctaaa ccatcggata aggcattcac gccgcatccg
    17821 acatcttttg cctgatgctt cgtatttacg ccccaaccac cttcgccacc gcgtgggcga
    17881 aacgttgcat cccttcatcg atatccgcat cttccaccac cagcgacggt gcaaaacgca
    17941 tcacatccgg tccggcattc agcaccatta cgccagcctc tgcgcccgca tacaggaaat
    18001 cacgcgcccg acctttgtac tgtggtttca gctctgcgcc aatcaacagc cccataccgc
    18061 gaatatcgct aaacacatcg tactgctgat cgatcttctg cagatggtca acaaaacgct
    18121 ggcgtttcgc ctgaatgcct tccagcactt cagggggatt gatgatatca aacgccgccc
    18181 ccgctactgc acaggccaga ggattaccgc cgtaggtgga accgtgagaa ccaggatgaa
    18241 acgcagaagc aatttccgcc gtggtcagca tggcgctaat cgggaagccg ccgcctaacg
    18301 ctttcgcaga ggtcagaata tccggcgtca cgccgtagtg catgtaagca aacaaatcgc
    18361 cggtccgccc catcccgcac tgcacttcat caaacaccaa taatgcctga tgttgatcgc
    18421 acagctcgcg caagccctgc aaaaactctg gcgtcgcagc cgtcacgccg ccctcgccct
    18481 ggatcggctc aaccaccacc gcacaggtgt gatcatccat cactgctttc actgcgtgga
    18541 gatcgttaaa gggaacgtgg atgatgtctg ccggtttcgg cccaaagccg tcggaatatt
    18601 ttggctgccc acccaccgaa acggtaaaca gcgagcgacc atgaaaagcg ttatggaagg
    18661 caataatttt ggttttgaac gggctatgac gcacacaggc gtaatgacgt gccagtttaa
    18721 aggcggtttc gttagcttcc gtgccggagt tcataaacac cacgcgttcg gcaaacgttg
    18781 cctcaatcag tttacgccca agacgcagcg ccggttcatt ggtgaaaacg ttactgatat
    18841 gccacagaat ttcgccctgg gtttttaacg cgttcaccag cgcaggatgg caatggccca
    18901 acgccgtaac tgcaatgcca cccgcgaaat cgacatactc cttgccttgc tgatcccaga
    18961 ttcggctgcc ctgacctttt accggaataa actctgccgg agcataaatc ggcaggatca
    19021 cttcatcgaa agtcgcgcgt gtaattgctg tttgttcaat tgccatctca tgatcaccct
    19081 gttacgcata aacaaatgtg aaattataat cacaaaatat gcataaaaaa tcactaaatg
    19141 gcaatcagaa atcagcgatg caggaaatta gccagcagtt gatgtccttg ttcgctaaga
    19201 atactttctg gatggaactg cacaccttcc agatcccact ggcgatggcg aatccccata
    19261 atctcgcggg tttcgctcca ggccgtcacg tcaaagcacg ctggtaatga gtcaggttcc
    19321 accaccagcg aatggtagcg tgtcacggta agtggatttg ccagcccccg aaatacgccc
    19381 tcaccgctat gtgtaatcgg cgaggttttg ccgtgcatga cctttgcggc gcgcacaact
    19441 ttgccgccaa atgcctgcgc cattgcctga tgaccgaggc agacgccaag aatcggcaag
    19501 cgcccggcat agtgacgaat aacgtcaagg gagatcccgg cttcatctgg cgtacagggg
    19561 ccaggtgaga tgacaatttt ttgtggttta agggcgtcga tatccgccag cgtcaacgca
    19621 tcgttgcgct taaccagcac atccgccccc agttcacaaa agtactggta gaggttccag
    19681 gtaaaagaat cgtagttatc tataagcagg atcatggcgg ctccggtacg aaagaaccgc
    19741 gctattttac tcagattccc cggctttgct taccactttg ctaaatatca tctgcagtgc
    19801 ggtgagatcc cccattgccc cactctgatt tgcctgattc caggcctctt tttcgatacc
    19861 ctgccagctc agttgatatc cggcatgaat cgccagttgc tcgaagaaga tccgctgtgc
    19921 cagaccactt cccacccgga agggatgcag cacgttgatt tcacaatagt aatgcgccag
    19981 ccgctcgacg aacttcgctt tctccaggcc aaccagataa ccttcttcct ccagatcctg
    20041 catcagggca ttgccctctt tttcgatata agcaaagtgg cagaacggcg tatcgccctg
    20101 ataaatatca acttcacgca gttgccctgc ccagtcgaaa atatcctggt acagctggcg
    20161 atggatagtg cgcaaatgcg gtaaaccgcg caccagcgga ccaagctcaa tggtcgcagc
    20221 acgcagcgcc gtcatttcgt aagcggcctg ttccagccgc tgctgctgac ggatgttcag
    20281 ccggttacgc atgatatcaa ggccaggata aagatacgga tcgcgccctt cgccgaattt
    20341 atcgctcata gtgcctcctc agctcttcaa ggcgcgctaa agcctctgcg gcagtaagag
    20401 tgactaaagg catctcgacg ccttcaagac ggcgactggc ctggaaatta cgattacgct
    20461 gaagctccca gagacgggac ttttgcttat cggtgagttt cttcacttga tgcctccctg
    20521 aacgtgtcca tttgccacaa gtataagcgg caaatccggg ttacgccggg agagcagaag
    20581 cgcgggcaag attgcccgcg cgagaaatca ttacggcagg actttagcgg aaaggataac
    20641 taccggtttt gacggcacat tctggtacgg accaacatca tgagtcggca cctgggaaat
    20701 cttatcggca acgtccatgc ctttcaccac tttaccaaat accgcgtaac cgaaatcacg
    20761 ctgaccatgg tcaaggaagg cgttatcggc aacgttgata aagaactggc tggtggcgct
    20821 gtctttgtca gcggtacgcg ccatcgcgat ggtgccacgc gtgttgcgca ggccgttatc
    20881 ggcttcattt ttgattggcg ggtttggttt tttctgctgc atctgctcgg tgaaaccgcc
    20941 gccctgaatc ataaagccag gaatgacgcg gtgaaaggta gtgttgttat aaaaaccgct
    21001 gttcacataa tcgacaaagt tttgcacaga cactggcgct ttttgtttat ccagctccag
    21061 ttcgatgtta ccagctgagg ttgtcaacaa tacgtgcggg tcccctttcg ctgccattgc
    21121 tgcgggagaa agagcagaaa gagcgaaaac agcagccatc gccgccaggg tcgatttgaa
    21181 catgagattt ccttaacaaa cagagcagaa aaaaagcaag tgaagagatt ctaaatagcc
    21241 ttcaagaaca aggccatccc tttacctaat tttacgtatc tgaaatatat gtaacctgac
    21301 gatcactttt tattccgtga tcaaaatcac ctcttaaaat gcaatttagc aaccgattgc
    21361 aataaaacat ttaaacagat cacaaaatca cctaaaatcg cccgctcgcg gcatatcccg
    21421 ctatgccatt ttatttttga acgcaggcca atcatgacta acagcaatcg catcaagctc
    21481 acatggatta gctttctctc ctacgcactg actggtgcgt tggttattgt caccgggatg
    21541 gtgatgggaa atatcgccga ttatttcaat ctgcctgttt ccagtatgag taacaccttc
    21601 accttcctca acgccggcat tttaatctct atcttcctca acgcctggct gatggaaatc
    21661 gtcccgttga aaacgcagtt acgttttggc tttctcctga tggtgctggc ggttgccggt
    21721 ttgatgttca gccacagcct ggcgctgttc tcgacggcga tgttcattct cggggtggtc
    21781 agcggcatta ctatgtcgat tggtacattc ctgataacac aaatgtatga agggcgtcag
    21841 cgcggttccc gcctgttatt tactgactcc ttcttcagta tggctgggat gattttccca
    21901 atgatcgccg cgtttctact ggcgcgcagc attgagtggt actgggttta tgcctgcatc
    21961 gggctggtgt atgtcgctat ttttattctg accttcggct gtgagttccc ggcactgggc
    22021 aaacatgcac caaaaacgga tgctccggta gagaaagaaa agtgggggat cggcgtactg
    22081 tttctctctg ttgcggcgct gtgctacatc ctcggtcagt taggttttat ctcctgggtg
    22141 cctgagtatg ccaaaggcct gggcatgagc ctgaacgacg cgggcacgct ggtgagtaac
    22201 ttctggatgt catacatggt cggcatgtgg gcgttcagct ttattcttcg cttctttgat
    22261 ttgcaacgca ttctgaccgt actggctggt ctggctgcga ttctgatgta cgtctttaac
    22321 accggaacac cagcacatat ggcgtggtca attctcgctc tgggcttctt ctccagcgcg
    22381 atctatacca ccatcatcac cctgggttca cagcagacca aagtaccgtc gccaaaactg
    22441 gttaactttg tcctgacctg cggaaccatc ggtactatgt tgacctttgt ggttaccggc
    22501 ccgattgttg aacatagcgg tccgcaggcg gcactgctta cagcaaacgg tctgtacgct
    22561 gtcgtctttg tgatgtgctt cctgttaggt ttcgtcagcc gtcaccgtca gcataacacc
    22621 ctgacctctc attaattgct aatgccggac ggcactatcg tcgtccggcc ttttcctctc
    22681 ttcccccgct acgtgcatct atttctataa acccgttcat tttgtccatt ttttgcacaa
    22741 acatgaaatg tcagacaatt ccgtgactta agaaaattta tacaaatcag caacatacca
    22801 cttaaggagt atataaaggt gaatttgatt tacatcaata agcggggttg ctgaatcgtt
    22861 aaggtaggcg gtaatagaaa agaaatcgag gcaaaaatga gcaaagtcag actcgcaatt
    22921 atcggtaacg gtatggtcgg ccatcgcttt atcgaagatc ttcttgataa atctgatgcg
    22981 gccaactttg atattaccgt tttctgtgaa gaaccgcgca tcgcttatga ccgcgtacac
    23041 ctctcgtctt acttctctca ccacaccgcc gaagagctgt cgctggtgcg cgaaggcttc
    23101 tacgagaaac acggcatcaa agttctggtc ggcgaacgcg ctatcaccat caaccgtcag
    23161 gagaaggtga ttcactccag cgccggacgt accgtttttt atgacaagct gatcatggca
    23221 accggttcct acccgtggat cccaccaatc aaaggttctg atactcagga ctgctttgtc
    23281 tatcgcacca ttgaagacct caacgccatt gaatcctgcg cccgtcgcag caaacgcggt
    23341 gccgttgttg gtggcggcct gttaggtctg gaagccgctg gcgcgctgaa aaacttaggt
    23401 attgaaaccc acgttatcga atttgcccct atgctgatgg cggaacagct tgatcagatg
    23461 ggtggcgagc agctgcgtcg caaaatcgaa agtatgggcg tgcgcgttca caccagcaaa
    23521 aacacccttg agattgtgca ggaaggtgtc gaagcgcgta aaaccatgcg ttttgccgac
    23581 ggcagcgaac tggaagtcga ctttatcgtc ttctctaccg gtatccgtcc gcgcgataag
    23641 ctggcaaccc agtgtggtct ggacgttgct ccgcgtgggg gtatcgtcat taatgattcc
    23701 tgccagactt ccgatccgga tatctacgcc atcggtgaat gcgcaagctg gaacaaccgt
    23761 gtatttggtc tggtagcacc tggctacaaa atggcgcagg tcgccgttga ccatattctc
    23821 ggtagcgaaa acgcctttga aggtgctgac cttagcgcca agctgaaact gctgggcgta
    23881 gacgtaggcg gtattggtga tgcgcacggt cgcacgcctg gcgcacgtag ctacgtttac
    23941 ctcgacgaaa gtaaagagat ctacaaacgc ctgattgtca gcgaagacaa caaaaccctg
    24001 ctcggtgcgg tactggtggg cgataccagc gactacggta acctgctgca actggtgctg
    24061 aacgctatcg aactgccgga aaacccggat tccctgatcc tgccagcaca ctctggtagc
    24121 ggcaagccgt ctatcggtgt tgataaactg ccggacagcg cgcaaatctg ctcctgcttc
    24181 gacgtcacta aaggtgatct gattgctgcc atcaacaaag gctgccacac agttgcggcg
    24241 ctgaaagctg aaaccaaagc gggtactggc tgcggtggct gtatcccgct ggtcactcag
    24301 gtactgaacg cggaactggc gaaacagggc atcgaagtta acaacaacct gtgcgaacac
    24361 tttgcttatt cgcgtcagga actgttccat ttgatccgcg ttgaaggcat taaaaccttc
    24421 gaagaactgc tggcgaaaca cggcaaaggc tacggttgtg aagtttgtaa accaaccgtc
    24481 ggttcgctgc tggcctcctg ctggaacgaa tacattctga agccggaaca tactccgctg
    24541 caggattcta acgacaactt cctcgctaac atccagaaag acggcaccta ctcggtgatc
    24601 ccgcgttctc cgggcggtga aatcaccccg gaagggctga tggcagtggg tcgtatcgcg
    24661 cgtgaattta atctctacac caaaatcacc ggctcccagc gcctggcgat gtttggcgca
    24721 cagaaagacg atctgccgga aatctggcgt cagctgattg aagctggctt cgaaaccggt
    24781 catgcctatg cgaaagcact gcgtatggcg aaaacctgcg tgggtagcac ctggtgccgc
    24841 tacggtgttg gcgatagcgt cggcctcggc gtggaactgg aaaaccgcta caaaggcatc
    24901 cgtacgccgc acaaaatgaa gttcggtgtc tccggctgta cccgtgaatg ttcagaagct
    24961 cagggtaaag acgtgggtat tatcgccact gaaaaaggct ggaacctgta tgtttgcggt
    25021 aacggcggca tgaaaccgcg tcatgcggat ctgctggcag cggatatcga tcgcgaaacg
    25081 ctgatcaaat atctcgaccg cttcatgatg ttctacatcc gtaccgccga caaactgacg
    25141 cgtaccgcgc cgtggttaga aaacctcgaa ggcggcatcg attacctgaa agcggtgatc
    25201 attgacgaca aactggggct gaacgctcat ctggaagaag agatggcgcg cctgcgtgaa
    25261 gcggtagtgt gtgagtggac tgaaacggtc aatacgccgt ctgcgcagac tcgcttcaaa
    25321 cacttcatca acagcgacaa gcgtgacccg aacgtgcaga tggtgccaga gcgcgaacag
    25381 caccgtccgg caacgccgta tgaacgtatc ccggtaactc tggtggagga caacgcatga
    25441 gccagtggaa agacatctgc aaaatcgatg acatcctgcc tgaaaccggc gtctgcgcgc
    25501 tgttaggtga cgagcaggta gcgattttcc gcccgtatca cagcgaccag gtgtttgcga
    25561 tcagcaacat tgatccgttc ttcgagtcca gcgtgctatc acgcgggctg attgcggaac
    25621 accagggcga gctgtgggtc gccagcccgc tgaaaaaaca gcgttttcgc ttaagtgatg
    25681 gcttgtgcat ggaagacgaa cagttttccg tcaaacatta cgaagcgcga gtgaaagacg
    25741 gcgtggtgca gctgcgcggt taatgtttta acgggaggcg caatgcctcc cctttttgca
    25801 tggtcctgta atgaccttcg gtatattgca gaacattttt taaacttttt gttttatttt
    25861 ttgtttttat tttttaaagg ataatcaaat gtttacagac actattaata agtgtgcggc
    25921 taatgctgcg cgcattgcac gcctgtcggc aaataacccg ctcggctttt gggtcagctc
    25981 cgccatggcg ggcgcatatg tgggtcttgg gatcatcctg attttcacgc tcggtaattt
    26041 gctcgacccg tccgtacgtc cgctggtgat gggcgcgacc tttggtatcg ccttaacgct
    26101 ggtgattatc gccggttctg aactgttcac cggacacgcc atgttcctca cctttggggt
    26161 aaaagcgggc agcatcagcc acgggcaaat gtgggcaatc ctgccgcaaa cctggctggg
    26221 taacctggtc ggttccgtct tcgttgccat gctctatagc tggggcggcg gtagcctgct
    26281 gccggtagat accagcatcg ttcactccgt cgcgctggct aaaaccactg caccggcaat
    26341 ggtactgttc ttcaaaggtg cattgtgtaa ctggctggtt tgcctggcta tctggatggc
    26401 gctgcgcact gaaggggcgg cgaaatttat cgctatctgg tggtgtctgc tggcatttat
    26461 cgcgtccggc tacgagcact ccatcgctaa catgacgctg ttcgccctct cctggttcgg
    26521 caaccacagc gaagcctaca cgctggcggg tattggtcat aacctgctgt gggtgacgct
    26581 gggtaatact ttatcgggtg ccgtattcat gggattgggt tattggtatg ctacgccgaa
    26641 agcgaatcgt ccggttgcgg acaaatttaa tcaaactgaa acggctgccg gttaattact
    26701 aaggggtttt tacgtggatc atttgcctat attttgccaa ttacgcgatc gcgactgtct
    26761 gattgtcggc ggtggtgatg tcgcggaacg caaagcaagg ttgctgttag acgcaggcgc
    26821 tcgcttaacg gtgaatgcat tagcgtttat tccacagttc accgcatggg cagatgcagg
    26881 catgttaacc ctcgtcgaag ggccatttga tgaaagcctt ctcgacacct gctggctggc
    26941 gattgcagcg acggatgatg acacgcttaa ccagcgcgtc agcgaagccg ctgaagctcg
    27001 tcgcatcttc tgtaacgtag tcgatgcgcc gaaagccgcc agctttatta tgccgtcgat
    27061 tattgaccgc tcaccgctaa tggtagcggt ctcctctggc ggcacctctc cggttctggc
    27121 gcgcctgttg cgcgaaaaac tcgaatcact gctgccgttg catctgggcc aggtggcgaa
    27181 atatgccggg caattacgcg ggcgagtgaa acaacagttc gccacgatga gtgagcgtcg
    27241 ccgtttctgg gagaaattgt tcgttaacga ccgcctggcg cagtcgctgg caaacaacga
    27301 tcagaaagcc attactgaaa cgaccgaaca gttaatcaac gaaccgctcg accatcgcgg
    27361 tgaagtggtg ctggttggtg caggtccggg cgatgccggg ctgctgacgc tgaaaggact
    27421 gcaacaaatt cagcaggcag atgtggtggt ctacgaccgt ctggtttctg acgatattat
    27481 gaatctggta cgccgcgatg ctgatcgcgt tttcgtcggc aaacgcgcgg gataccactg
    27541 tgtaccgcag gaagagatta accagatcct gctgcgggaa gcgcaaaaag gcaaacgcgt
    27601 ggtgcggctg aaaggtggcg atccgtttat ttttggccgt ggtggcgaag agctggaaac
    27661 actgtgcaac gcgggtattc cgttctcggt ggttccgggt attaccgcag cttctggttg
    27721 ctctgcctat tcgggtattc cactcacgca tcgcgattat gcccagagcg tacgcttaat
    27781 taccggacac ttaaaaaccg gtggcgagct ggactgggaa aacctggcgg cagaaaaaca
    27841 gacgctggtg ttctatatgg gattgaatca ggccgcgact attcagcaaa agctgattga
    27901 acacggtatg cctggcgaaa tgccggtggc aattgtcgaa aacggaacgg cagtcacgca
    27961 gcgcgtgatt gacggtacgc tcacgcaact cggtgaactt gctcagcaaa tgaacagtcc
    28021 atcgctaatt attattggtc gggttgttgg cctgcgcgat aaactgaact ggttctccaa
    28081 ccattaattt aacccgggcc agagaattct ggccttctta atgactcttt ttatatattc
    28141 agcaaataaa acataacccc ttaataaata ttatctgcta atgggttata ttcaccgtta
    28201 cagcgactta caaaattaag tcattcgctc actgacgcca attaaatatt cctgtatcgt
    28261 tcttaacgag cagtctgctt atattcatat aatcaatgaa tattaattaa tatataatac
    28321 atagggaatg taaatgaaca aatttattaa agttgcactg gtaggtgcag tactggctac
    28381 gttaactgca tgtactggtc atattgaaaa ccgtgataag aactgctctt acgactacct
    28441 gctgcacccg gcaatttcta tttctaaaat cattggcggt tgcggtccta ctgcacagta
    28501 agtctctgac agaaatcggc taacaccggt ttaattgccc gacgccctct ggattttccg
    28561 agggcgtatt tgtttctatg tccttcttgc ccccgcccgt aaaaatattt tcactttaaa
    28621 ttcaatttga taactacatc cattagccac agcgcgatcc tgtgcgaaat tttgtgatct
    28681 tcctccacat tacataacat catatgttgt tatattcatc atgcattgtc atgttacctt
    28741 ttaaatgact gcaaactctc ccctacaacg tattggacaa gaaaaaggta tcgctatggg
    28801 aagccaggaa ctccaacgca agctcggatt ttgggccgtt cttgcaatcg ccgtcgggac
    28861 aaccgtcggc tccggtattt ttgtatctgt gggtgaagtg gcaaaagcag cgggcacgcc
    28921 gtggcttacg gtgctcgcgt ttgtcattgg cgggttaatt gtgatcccgc aaatgtgcgt
    28981 ctatgcggaa ctatccaccg cttatccgga aaatggcgca gattatgttt atctgaaaaa
    29041 tgccggaagc cgaccgctgg ctttcctctc cggctgggcc agcttctggg ccaacgatgc
    29101 accgtcattg tcgattatgg cgctggcgat tgtcagcaat cttggctttt taacgcctat
    29161 cgatccgttg ctcggtaaat ttatcgccgc cggattaatt atcgccttta tgttgctaca
    29221 cctgcgctcc gttgaaggcg gcgcagcgtt tcagacgcta attaccatcg ccaaaattat
    29281 cccgttcact atcgtcattg gccttgggat cttctggttt aaagcggaga attttgccgc
    29341 ccctaccacc actgcgattg gcgcaacggg cagctttatg gcgctgctgg cggggatctc
    29401 tgccaccagt tggtcgtata ccggcatggc ctctatctgt tatatgaccg gcgaaattaa
    29461 aaaccccgga aaaaccatgc cacgagcgct gattggttcc tgtctgctgg ttctggtgct
    29521 ctacaccctg ctggcgctgg tgatttccgg cctgatgccc ttcgacaaac tcgccaattc
    29581 tgaaacgccg atttccgacg ccctgacctg gatccccgca ctcggcagca ccgctgggat
    29641 ctttgttgcc atcacggcga tgatcgtcat tcttggttcg ctttccagct gcgtgatgta
    29701 ccagccgcgg ctggaatacg cgatggcgaa agacaacctg ttctttaaat gcttcggcca
    29761 tgtgcatccg aaatacaaca cgccggatgt ctccatcatc ctgcaagggg cgctggggat
    29821 cttcttcatc ttcgtttccg atctcaccag cctgctgggt tatttcaccc tggtgatgtg
    29881 tttcaaaaat accctcacct tcggctccat catctggtgt cgtaaacgcg acgattacaa
    29941 accgctgtgg cgtactccgg ctttcgggct gatgaccacc ctcgccattg cgtcaagcct
    30001 cattctggtc gcctcaacct ttgtctgggc accgattccc ggccttatct gcgccgtcat
    30061 cgttattgct actggtctgc ctgcttacgc cttctgggcg aagcgtagcc gccagctcaa
    30121 cgctttgtcg taatgttatc tggagaaaat aaaatgttgg atattgataa aagcaccgtg
    30181 gactttctgg tcaccgaaaa tatggttcag gaagtggaaa aagttctcag ccatgacgtt
    30241 ccgctggtgc acgccatcgt ggaagagatg gtgaagcgcg acattgatcg tatttatttc
    30301 gttgcctgcg gctcgccact caacgcggcg caaacggcga aacatctggc ggatcgcttt
    30361 tccgatcttc aggtctacgc catttccggc tgggagttct gcgataacac cccgtatcgc
    30421 ctcgacgatc gttgcgcggt gattggcgtt tctgactacg gtaaaacgga agaggtaatc
    30481 aaagcgctgg agctgggccg ggcctgcggc gcactcactg cggcgttcac caaacgcgcg
    30541 gacagcccga ttacctcggc ggcggaattt agcattgatt atcaggccga ctgtatctgg
    30601 gaaattcacc tgctgctctg ctacagcgtg gtgctggaga tgatcacccg cctcgcgccg
    30661 aacgcggaaa tcggcaagat caaaaacgat ctcaagccgt tgccgaatgc gctcggtcat
    30721 ctggtacgca cctgggaaga aaaaggccgc cagcttggtg aactggccag ccagtggccg
    30781 atgatttata ccgttgctgc gggtccgctg cgtccgctgg gttacaaaga aggcattgta
    30841 acgctgatgg aatttacctg gacgcacggc tgcgtgattg agagcggaga gttccgccat
    30901 ggcccgctgg agattgtcga accgggcgtt ccgttcctgt tcctgctcgg caatgatgaa
    30961 agtcgccaca ccaccgaacg cgccattaac tttgttaaac agcgtactga caacgtgatc
    31021 gtcatcgatt acgccgaaat ttcgcaaggg ctgcacccgt ggctggcacc gttcctgatg
    31081 ttcgtgccaa tggagtggct ctgctactac ctgtctattt acaaagatca caacccggat
    31141 gaacgccgct attacggtgg tctggtggaa tattaatccc tctcccggcc cggcaacggg
    31201 ccggatttac gcaaggagtt acccgatgaa aacaggtatg tttacctgcg gccaccagcg
    31261 gctgcctatt gaacacgcat ttcgtgatgc aagcgagctg ggttacgacg gcatcgaaat
    31321 ttggggcggt cgcccgcacg cgttcgcgcc ggacttaaaa gcgggcggca tcaaacaaat
    31381 caaggcgctg gcgcagacgt atcagatgcc gattatcggc tatacgccag aaaccaacgg
    31441 ctatccgtat aacatgatgc tgggcgatga acatatgcgt cgcgaaagcc tcgacatgat
    31501 caagctggcg atggatatgg caaaagagat gaacgcgggt tatacgctga tttccgcggc
    31561 ccacgcaggc tatctcacgc cacctaatgt tatctgggga cggctggcag agaacctaag
    31621 cgaactgtgt gagtacgcgg aaaacatcgg catggatcta atcctcgaac cgttaacgcc
    31681 gtatgaatcg aacgtcgtgt gtaatgctaa tgatgtgctt catgcgctgg cgctggtgcc
    31741 ttcgccgcgc ctgttcagca tggtcgacat ttgcgcgccg tatgtccagg cggaaccggt
    31801 gatgagttat ttcgacaaac tgggcgataa attatcgtca tctgcatatt gtcgacagcg
    31861 acggggccag cgacacgcat tacattcctg gagaaggcaa aatgccgctg cgggaactga
    31921 tgcgcgatat tattgagcgg ggctatgagg gttactgtac ggtggagctg gtgacgatgt
    31981 atatgaacga gcccagactc tatgcccgcc aggcgctgga acgctttcgc gcgctgctgc
    32041 cggaggatga gagatgaaaa ccctggcgac aatcggcgat aactgcgtcg atatctaccc
    32101 gcaactgaat aaagcgtttt ctggcggtaa tgcggtcaat gtggcggtgt actgcactcg
    32161 ctacggcata cagccgggat gcattacctt ggtgggtgac gatgactacg gcacaaagct
    32221 gaagcaggat ctcgcccgca tgggcgtcga tatcagccat gtccatacga aacacggcgt
    32281 taccgcacaa actcaggtgg aactgcacga caatgatcgc gtttttggcg actacaccga
    32341 aggcgtgatg gccgactttg ccctgagtga agaggattac gcctggctgg cgcagtatga
    32401 cattgtgcac gcggcaatct ggggacatgc ggaagacgca ttcccacagc tgcacgctgc
    32461 gggcaaactt accgctttcg acttctccga caagtgggac agcccgctct ggcagacact
    32521 ggtgccgcat ctcgattttg cctttgcctc cgcaccgcaa gaagacgaag cgctgcgtct
    32581 gaagatgaaa gcgattgttg cccgtggcgc aggaacagtg attgtcacgc tgggtgaaaa
    32641 cggcagcatt gcctgggatg gcgcgcagtt ctggcgtcag gctcctgaac cggtgacggt
    32701 tatcgacacc atgggtgccg gagattcgtt cattgccgga ttcctttgcg gctggtctgc
    32761 ggggatgaca ttaccgcagg cgatggcgca gggaacggcg tgcgcggcga aaaccattca
    32821 gtatcacggt gcctggtagg tataacgttg gcgtgagcat ctccgcgcca acgtgctgtt
    32881 acttgccgga aaacgaccct ataatccgtg taattcattc tttatttcag ggtcgattat
    32941 gtcagctacg gaccgctact ctcatcaact cctctacgct accgtccgcc agcggctgct
    33001 ggatgatatc gcgcaggggg tttaccaggc cgggcaacag atccctaccg aaaacgagct
    33061 ttgtacgcaa tataacgtca gccgcattac tattcgcaaa gctatcagcg acttagtggc
    33121 agacggcgta ctgatccgct ggcagggcaa aggcaccttt gtacaaagcc agaaagttga
    33181 aaacgccctg cttactgtca gtggttttac cgattttggc gtctcacaag gcaaggcgac
    33241 gaaagagaaa gtgatcgaac aggaacgggt cagcgccgcg ccgttttgcg aaaagctgaa
    33301 catccccgga aacagcgaag tgttccatct ctgccgggtg atgtatctcg ataaagagcc
    33361 gctgtttatt gatagttcat ggatcccgct gtcgcgttat cctgactttg atgagattta
    33421 cgtcgaagga agctccacct atcagttatt tcaggagcgt tttgacacgc gagtggtcag
    33481 cgacaaaaag accatcgata tctttgccgc cacccgcccg caggcaaaat ggctgaaatg
    33541 cgaactgggc gaaccgttgt ttcgcatcag caaaatcgcc tttgatcaga atgacaaacc
    33601 ggtgcacgtc tccgaactct tctgccgcgc caatcgcatc accttaacga ttgataataa
    33661 aagacattaa ccgtaggccg gataagatgc gccagcatcg catccggcga tgctggcgcg
    33721 ttgaatttta catcccgtac gttcccctca ccctaaccct ctccccaaag gggcgagggg
    33781 accgttcagt acggaattta gcttgacggt gctatccagc attaaatact ggcaatactc
    33841 tcacgcaaaa tccgcagcac cgtctcttca ccgttgcgat tcgggttaat gcgaatcgca
    33901 caatgttctg attgtggatt cgcctggcga aacgttccgg aaaggcgata aaagagcggc
    33961 gggatttcat atttcgactc tgcacccacc gggtaaggca aggcaccgcg cttttgcgcc
    34021 tcttccagca ctctggcggc aatcggctga tgaaactcga caatcaacac cttcgactgc
    34081 gcattagcaa tcaccgcgct tttcacttcc ggcaccgcac caccgttaag caaagccagc
    34141 aaccgttcag atacccccgc ctgcaccgcg tgcatcactg gcgcaaacac cagcccacac
    34201 aagacttcca gcgcctgagc gccctgaatc tggctgccgc cggaatagag cgtggagcga
    34261 atccgactaa taacatcggc atcgcctacc accgcgccaa caccctccgg cccaaacagc
    34321 ttgaagcagg aaaaagtcga gacatttgcc ccgcattcgc agccaattcg cgccactttc
    34381 atcaccgcat agttgtcatc ggttaatgca ggaacgccag ccgagcgcaa tgttgccagc
    34441 acatctgcca gaatgtagcc gtcctgcggc tgctggcgcg tatgctgcac cagcgccgca
    34501 tccggtagtt gctcgtcgac gacctgcttc agtgccgaca ggtcattgaa atcagcagta
    34561 ataagcgtca gccccatctg ctcaataatg acctgtgtcg tcgggtaaac aggcgcatca
    34621 tgtaccagaa gacgctgccc cggtttgagc aaagccgcca acgccgcacg aatcgcgcca
    34681 gtacccgcac cctgcaccag cgccgcagcc tgtgcgtgaa atgcatcagc cagcacctgt
    34741 tccacacgct gggtaacgcg cggttgattc agtcctggcg ttaagcctaa atcaccgcca
    34801 gtaagaaact cgctgccggg gaaatggcga caaatgctat ccaccagcgc aaactgcttt
    34861 tgctgcgcct caataatcgt cagactttgc agaggaaacg tcttcatagg tgccttcctt
    34921 aagccggaac gaacaggcca agccagtaaa gaacattcag cagaataccg gtgatcatta
    34981 ctgccacgac cggtgccgcc attttctgta ccggacggcc cagggattcg ttgaggaagt
    35041 aaatggcaac cgcgatagag aatccggtat aacccgccat cttaattgct gcgaaaatcg
    35101 aaccgaccag cagcgccact tccatcagca tattcatggc gttacggatg ttatccgacg
    35161 cattacgcac cgacgggtag cgtcccagcc atttgccgat cgaacgaagc agcaagactt
    35221 ccgccgaaat aaccactgcg cctaataccg ctgcaactat cggattcggc gagagatagc
    35281 ccaccgcata aacaaaggta aagcccgcaa ctgcatacac acccgttgct aaggcggtgg
    35341 tggcaatcaa cggtacaaaa cccagcccgc gcataaattc tgccagtgcc gcctggttga
    35401 tcagcgtttg cgattgttcc ggcgttacgc ctgcggagta cgctttctcc agagtgaaga
    35461 tcgacacttc actgccggcg aaaatcttca tgctggcaac ggcggcaatc aatgctccca
    35521 cgatggcgat atagggtaag tttttgataa tccgcgacgt gcgttcttca aacaccgaaa
    35581 gcccgctggc atcaatatca ttttcatcac gatgacgcag gtcgtgagtt atcgcgatcc
    35641 ccagcagcat caccatgcca ataaagattt cgatggattc agggttaaga tgtgggaaat
    35701 agcgcacgac taccacacgg gtcatcagaa caaccacggc ggcgaccaga ctttgcttcc
    35761 agccaaactg gtagaaaatc gccaccagcg ggaacagtgc aaaagcagaa accaccggcg
    35821 agcttaattc cccgaggcta cctaatacat ccaccggcag cgcggtcagc agctggttta
    35881 ctggcaacag gcaagtaagg atcaacacgc cccagatagc gccaagacca aacgccatca
    35941 ggctgtttat cgccagtacg ccgaggatat cggtaggaag aaagagtaac catgcgttga
    36001 gcagcccggt tttcagggta aaagagatcc ccaccgaggc cacaaaacca atgctcagac
    36061 caaaagcgat actccccgcc tcgcgacggt tcatatagcc ttcaatcagt tgcggcagga
    36121 tcgggcggat accgtcatga aaaacagccg ccgagcgatg cgccagaagc gatgtcatac
    36181 ccgtcaggca cgccaccacg ataatctgaa tatacagatc catagtcgtg ccttttattt
    36241 cagatggtta atcagcatcg gaatcgcgtg ttcgacgtgc tcaacggaaa ggccaaacgc
    36301 cacttttcct tcagcgatca ttttggcgat atgctcgtct ttcgctttaa tgcctggttt
    36361 ggcaatggtg caacttttgt tatagccaat caccgcgatg gcaatcgaca atgccgcgcc
    36421 cgcaccggta ttacacgcac cgatgtagta atccagttgc ccggatttca ctttcattgc
    36481 cgcttccatg tcgttgtgaa tgaaaacttc aaaactgcca ggagccgtcg cttcaatggt
    36541 ttttttaatc tgctcacgct gtaagcctgc aacgccaatc tttttcatga gtctgtcctt
    36601 attggaaaaa ttgagaggga gtttcacgta acatcacatc cacatcggcc tgactgaatc
    36661 ctgactggcg caattgcgga ataaaggtgg tcaataaata gtcatagcca taaccaccat
    36721 tggcttttaa atgggagcgg cgcgtaatat ccatcgacag catgacgcgg ttcagcaacc
    36781 cacggtcacg tagcgcgtgg agcatcgcaa tgcgcttttc gtccgggtag taactgttct
    36841 taccaatggt gtcgaactgc acgtacgcgc cgagatcgat catcttcaaa atgttgtcga
    36901 ggttgtcttt cagatcgcag tgaccaacgg tgacgcgcga aagatcaact ccgtgggctt
    36961 gcaacagagc cagttgttcc agccccatgg tgctgaacga cgtatgcgtg gagatcgggc
    37021 gtccggtctg gttatgcgcc agcgcagcgg caataaatac cttctcttcc agcggcgtaa
    37081 tctttccttc gctggagccg atctccgcga tgatcccggc tttcagctcc gtgccatcga
    37141 taccctgttc aatttcatcg accatctcct gcgccagttc ctgcacgctg cgggtcacca
    37201 catgttccgg gaaaaacgca tcctggtaat aaccagtaca ggccaccacg ttgatcccgg
    37261 tttcgcgcat tacaccgagc ataaattgcg cattgcgccc catgtaacgg ttggtcatct
    37321 caatcacatt acgtacaccc cgggtcatca ggtcgttcat ctcctggcaa atgaacgcat
    37381 actgatcaag gcggcagtcc acgttgtttt taaagccgga gagatcaata tgcagatgct
    37441 catgggccag ggtgtaaccc gtcggatcaa aactcatagg gtgtctcctg caaaccggag
    37501 ggaggaaaga aaagagcgac cattttgtgg cggtggcgca cggaaaaatt cacacacggt
    37561 agctcccaca tcagaaagcg tggtgcgcac accgagctgc gtagcagcca ggccttgctg
    37621 ataaaccagc agcggcacca cttcgcgggt atggtggctg tgaccaatgg tcggatcgtt
    37681 gccgtgatcc gccatcacga ccaggcaatc atctggctgc atcgcatcaa caagccgggc
    37741 aaggttacgg tcaacgacct gcaaacgttc ggcataacgt gcgacgtctt ctgcatgacc
    37801 agcgaggtcg gtttcctgga tattggtgca aataaacgcc gtcggatggg tgttaaattc
    37861 gtcgagggtg atatccataa tccgctggct atccaccagg ttttgccagc tcacgccata
    37921 aggattgctg acgatatctg ccaccttacc caccagcacg gttggcacgc ccgcctcata
    37981 cagtttttgc ggcacctgca cttttttatc gacaccgtag cccatatgca cgacctggaa
    38041 gccgttgtca taagcgccag aacgcggcgc attgatacca ataaagcgcc cttctttgct
    38101 ttctgcggca tcgagaatgc gttgactgtc ggttaacagg ccaccaaatg taatgacccg
    38161 accgacctgt acctgctcac gcacgatacg accaattttg attgcgtcgt caaaagagat
    38221 cacagagaga ttggcggtaa tgttatagac ttggcctaaa tccgcctcga gattatcgcc
    38281 aatcgcaacc gcctgattga cccacagaaa ttacagttca tcgccacggc gctccacctg
    38341 ccaaccggcg gatactaatg cctgctcaac acggtcaatc acatcgcgaa aaggcatccg
    38401 cagcggcggt aacgggcgcg tgcctaaaat ttcctgatgc cccataaagg tatcgccacc
    38461 ttcatgttgc agctctgcca cgccccaggt tgcggaatct gacggctgca tatcgcctgg
    38521 cgcataaccc aatgcgttga ttagccccag cttctccagc gttggtagct gcaaatgcgg
    38581 caactggctc aggatgtgac cacatgtatt cgctcccgca tcttgcggac gcaccagcgt
    38641 gacatctttc attgccccta cgccaaagct atcaatcact aacaccacaa atcgcgccat
    38701 tacgccccca gagagtttcc aagactgtca taacgaccaa ctattttcgg ttcgccacgg
    38761 tgaataccgg acaccagcac cacatcgcta cgggtgacaa aaatctgcgt gcggaaacag
    38821 agcaccactg cactgcttac cggaaactcg cctgccagcg acagggtgta gtcgatactg
    38881 ctgtcatcca cagttttgag attggtttca gtaatctttt gattttctgg cgtaaacacc
    38941 aacgcatgtt gcgcatgacc acgacggtag taaccgccgc cgtagcagta gctgtcgcca
    39001 cggaaatgat gggagatttc actcaaccag agcatcgcga tacgttcaga ctgatcgccc
    39061 tgctggtttg ccggaatagt gcccgtcagc gcatgaccgg gttcggcatg agtcacaccg
    39121 tattacgcca gtaatggcag cgaagtgcag ctggtcgctg aaggcgcgtt cagttgctca
    39181 agtgcaatac cagatttcgc cagttgatcc cgtgcctgta tcagcgtgtg aagattcggt
    39241 gtcggcaaaa cttttccggc agcctcatcc caaagcaggc aagggaaatg ggtaagtccg
    39301 gctaaatgca gccccggcag tttcttaatt tcagcaacca cctcgtgcag cgaatgctgt
    39361 acaaaaccgc tctcctgccc cggataaaga aaatcatcgt cgctataaac tttgagcagc
    39421 acaaactgca cacgtccggc cttcaccgct gccgcagaaa cttcccgcgc tttgtcgaga
    39481 gtaaacacgg tgatgacgtc ggtgccctgt tcaacggcgt cagcaacctg atgacaaggg
    39541 atttgtacca gatgcccctg atgtgccacc gacaaaccag cgcggcgcat gactcgcgcc
    39601 tctttgtaat ccaccgccac aatgccgctg taacctaatg ccagcaattt ttccgccagc
    39661 catgggttac ggccaaactg cttggtcatc agatacagtt caatgccgta aagccgcgcc
    39721 gtctcaatca gtcgcttacc gttttccagt acctgatcca cgtcgatcac ccagctgtcc
    39781 ggggcgatct tgccctgctg ccacaaactt agtgcggcgg aaatcagcgc cgggttctgg
    39841 cgtttcaatg cttctacaaa catcttgttg cctcgtttct gttccttgaa ttaaatattc
    39901 atttttttga atatttaggt tgtgcgacgt ggatttgtcg ggagcttcaa acctcttcgt
    39961 tagcagccat ccataatcca taaaggttgg ccagcaaata gccttcttca cacgggttaa
    40021 cttccagtgc gaattccttc aacaacacct gatgcaattg cacaacggct tgccagtggc
    40081 tggattgcgc cagctctgcc agcaattcgt tatccagcgg ctctatttct tcaccacggc
    40141 gactgcgcat cagtgcactc gccatatgtg tcatcgccat cgttccttgc tcactgcgca
    40201 ccggcagatg gcactctgtt tccagtacgt tgacgacctg catcatgcct ttgcagatgt
    40261 ccttatcaat aacgcctgcc tcgcaaagca ggttgagtct ggtttccata tcagatgcct
    40321 ttcgtttaat aactcggttc ctgttcccca ctcactaccc gttgttgatg ggcgagcagg
    40381 gcgtgtttat ctacaacggc acgcagcagt tgttgcattg ggtaatcatc gactcgcgtc
    40441 aggaccacgg cttcggtggc ctgtaaaaat cgcggatcgt ctgtgagcgg cgtcgcctct
    40501 aatcccagca tggtcagttc gttttccgcc accacgttcc agataaccgc atcgacatcg
    40561 cctttaacaa tgcgttgtaa actctcgtga taagagagat cgacacgttc cacatcacta
    40621 tcgccaaaaa agacatcggt catgattttc tgatccgccg aacggttatc cagccccacg
    40681 cgcttcacgt ttgcggactc gcctttacgg caaatcaact ggtgctcgcc aacgtaggtg
    40741 tgcggcccca actccagcgc gaggcataac cctttttgcg tgagataact ttccgccgcc
    40801 agtcgggaaa ccaccgccat gtcatacacg ccgttaagta aacactccac gcgaatatcc
    40861 gcgccgcgca tatgcgcata gtaaaaagga atgccatcaa actgagcttt cagcccgctc
    40921 gctaaacctt cgtacaagcg ggtatagggc aaaggcatcg cacataccac gttgttgata
    40981 tccacatgac tcagcaatgc tttgttatcc atctcgacca gataactgcc attgcgccca
    41041 cggcgttcaa tccgtatcgc tccgcttgat tccaacgttt tcaacgcggc ctgcgtcagg
    41101 ccaaccgatg aacggcattc agttgccagc tcatctatgg ttttcagtcg attaccgcat
    41161 ttttcaccca acaaataacg ggccagcgtc gtgatgacga cgccttcttt tttgataaac
    41221 gttcgacgca taatatattt tc
//
LOCUS       DAZPDU010000016        39951 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZPDU010000016 DAZPDU010000000
VERSION     DAZPDU010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39951
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.016"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..102
                     /locus_tag="ACHURS_000784"
     CDS             <1..102
                     /locus_tag="ACHURS_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-f3"
                     /protein_id="HFR1236592.1"
                     /translation="THNDIMGGGGMSYAYEYDVKYNICHLRKKSRMR"
     gene            complement(189..864)
                     /locus_tag="ACHURS_000785"
                     /pseudo
     CDS             complement(189..864)
                     /locus_tag="ACHURS_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1009..1617)
                     /locus_tag="ACHURS_000786"
     CDS             complement(1009..1617)
                     /locus_tag="ACHURS_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFR1236593.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(1801..2118)
                     /gene="metJ"
                     /locus_tag="ACHURS_000787"
     CDS             complement(1801..2118)
                     /gene="metJ"
                     /locus_tag="ACHURS_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFR1236594.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            2395..3555
                     /gene="metB"
                     /locus_tag="ACHURS_000788"
     CDS             2395..3555
                     /gene="metB"
                     /locus_tag="ACHURS_000788"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFR1236595.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLSGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAVGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            3558..5990
                     /gene="metL"
                     /locus_tag="ACHURS_000789"
     CDS             3558..5990
                     /gene="metL"
                     /locus_tag="ACHURS_000789"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFR1236596.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTDFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(6129..6263)
                     /locus_tag="ACHURS_000790"
     CDS             complement(6129..6263)
                     /locus_tag="ACHURS_000790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1236597.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            6339..7229
                     /gene="metF"
                     /locus_tag="ACHURS_000791"
     CDS             6339..7229
                     /gene="metF"
                     /locus_tag="ACHURS_000791"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFR1236598.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            7558..9738
                     /gene="katG"
                     /locus_tag="ACHURS_000792"
     CDS             7558..9738
                     /gene="katG"
                     /locus_tag="ACHURS_000792"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFR1236599.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSERVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            9832..10737
                     /gene="yijE"
                     /locus_tag="ACHURS_000793"
     CDS             9832..10737
                     /gene="yijE"
                     /locus_tag="ACHURS_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFR1236600.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVMALALVSRKKKEAVSVKRI"
     gene            complement(10764..11381)
                     /locus_tag="ACHURS_000794"
     CDS             complement(10764..11381)
                     /locus_tag="ACHURS_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFR1236601.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQKRGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTHHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(11655..12758)
                     /gene="gldA"
                     /locus_tag="ACHURS_000795"
     CDS             complement(11655..12758)
                     /gene="gldA"
                     /locus_tag="ACHURS_000795"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFR1236602.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGGCSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAECE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(12769..13431)
                     /gene="fsa"
                     /locus_tag="ACHURS_000796"
     CDS             complement(12769..13431)
                     /gene="fsa"
                     /locus_tag="ACHURS_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFR1236603.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(13443..15944)
                     /gene="ptsP"
                     /locus_tag="ACHURS_000797"
     CDS             complement(13443..15944)
                     /gene="ptsP"
                     /locus_tag="ACHURS_000797"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFR1236604.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLHTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSTLE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLRQHLLAGVSEGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            16253..17332
                     /locus_tag="ACHURS_000798"
     CDS             16253..17332
                     /locus_tag="ACHURS_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFR1236605.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGTWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            17347..17667
                     /locus_tag="ACHURS_000799"
                     /pseudo
     CDS             17347..17667
                     /locus_tag="ACHURS_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
     gene            17718..20014
                     /locus_tag="ACHURS_000800"
                     /pseudo
     CDS             17718..20014
                     /locus_tag="ACHURS_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
     gene            19980..20858
                     /locus_tag="ACHURS_000801"
     CDS             19980..20858
                     /locus_tag="ACHURS_000801"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFR1236606.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            20860..21201
                     /locus_tag="ACHURS_000802"
     CDS             20860..21201
                     /locus_tag="ACHURS_000802"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFR1236607.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(21188..22039)
                     /locus_tag="ACHURS_000803"
     CDS             complement(21188..22039)
                     /locus_tag="ACHURS_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1236608.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(22254..23987)
                     /locus_tag="ACHURS_000804"
     CDS             complement(22254..23987)
                     /locus_tag="ACHURS_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFR1236609.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(24170..26821)
                     /gene="ppc"
                     /locus_tag="ACHURS_000805"
     CDS             complement(24170..26821)
                     /gene="ppc"
                     /locus_tag="ACHURS_000805"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFR1236610.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(27173..28324)
                     /gene="argE"
                     /locus_tag="ACHURS_000806"
     CDS             complement(27173..28324)
                     /gene="argE"
                     /locus_tag="ACHURS_000806"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFR1236611.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDTLRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            28478..29482
                     /gene="argC"
                     /locus_tag="ACHURS_000807"
     CDS             28478..29482
                     /gene="argC"
                     /locus_tag="ACHURS_000807"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFR1236612.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            29490..30266
                     /gene="argB"
                     /locus_tag="ACHURS_000808"
     CDS             29490..30266
                     /gene="argB"
                     /locus_tag="ACHURS_000808"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFR1236613.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSTLVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            30327..31700
                     /gene="argH"
                     /locus_tag="ACHURS_000809"
     CDS             30327..31700
                     /gene="argH"
                     /locus_tag="ACHURS_000809"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFR1236614.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            31967..32884
                     /gene="oxyR"
                     /locus_tag="ACHURS_000810"
     CDS             31967..32884
                     /gene="oxyR"
                     /locus_tag="ACHURS_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFR1236615.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGYFDKVLKQAV"
     gene            complement(32867..34267)
                     /gene="sthA"
                     /locus_tag="ACHURS_000811"
     CDS             complement(32867..34267)
                     /gene="sthA"
                     /locus_tag="ACHURS_000811"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFR1236616.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGVRVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            34588..35238
                     /gene="fabR"
                     /locus_tag="ACHURS_000812"
     CDS             34588..35238
                     /gene="fabR"
                     /locus_tag="ACHURS_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFR1236617.1"
                     /translation="MMGIRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            35238..35597
                     /locus_tag="ACHURS_000813"
     CDS             35238..35597
                     /locus_tag="ACHURS_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFR1236618.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(35637..36737)
                     /gene="trmA"
                     /locus_tag="ACHURS_000814"
     CDS             complement(35637..36737)
                     /gene="trmA"
                     /locus_tag="ACHURS_000814"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFR1236619.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPCILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      36851..37041
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            37106..38950
                     /gene="btuB"
                     /locus_tag="ACHURS_000815"
     CDS             37106..38950
                     /gene="btuB"
                     /locus_tag="ACHURS_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFR1236620.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            38895..39752
                     /gene="murI"
                     /locus_tag="ACHURS_000816"
     CDS             38895..39752
                     /gene="murI"
                     /locus_tag="ACHURS_000816"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFR1236621.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         9124 a        10041 c        11020 g         9766 t
ORIGIN      
        1 acccacaatg acataatggg gggcggggga atgtcatatg cctatgaata tgatgtaaaa
       61 tataatatat gtcatttgag aaaaaagagc agaatgaggt aaaaatcttt ggctgtgtat
      121 tcagtctggt aatcatccaa ggaaagctta tcagaagggt gatgactcca attagtagaa
      181 catatatttt cgataaaagc accgatgact ttttcatgaa gttctacaga acgcgagaaa
      241 cagatggttc tgcgggccag acgtttgatt cgggttctca gggtcagatt gttacgctca
      301 atgtgctgag taaaaatctt accagtcaga tgcttctcct tcggtaactc tctagcgtaa
      361 ctaccccatt cgtcagtggt caacatgccg atggtaaaag ggtaagcagc gcccgcaatt
      421 cacgacaggt ttcgtcagtc cgggggccaa aagtgtaggc cagaacacca ccagttttcg
      481 tgttgtacac gtaccagagc cagtgctgtc gcgctttact gccgacaaag ctccattgct
      541 gactaagttt gcagatgagt gccacatcag catgagcgac cggggatgac gttattcttc
      601 gtggcgagag ttttttaaag ttcggatgac tgtattaatg ccgattttca gcgtacgggc
      661 agtatcgcga accccggcac cgttgaaagt catttcagcg atttggtctt taactcccgg
      721 tttacggggc tcgtaacagt aactgagctg aaatacccgg tgacagtcac ggccgcggaa
      781 tcgctcatgt ctttttgggt tctgcccgtg acggtaaacc tgaacggact ggcaacgggg
      841 gcaatgaagg ttaactgtga ccatgaaaca accttaaaag cgagtattat atccttaatt
      901 caactaattg gaggcatcac cgtttaccgg agcgacagct tgcggaaagg tcgtgcaact
      961 acaaagcaac aaacagacta cggcgggaat tatttttttc atcatccatt accacgaaaa
     1021 cagcggccac tctttggcga ctgtggtgag ttgtggcgcg tcgaaaacgg cttgtggtga
     1081 aactaccgtt tcctcaagcg gaatattttt gccgtagcgt tctttgagct tagcctggac
     1141 cagcgggttg ctgagatcga actctttcaa cttctgctgc tcgcccacgc gcattcccag
     1201 cgcgttctgg taaaccttcc acaccacttc cgaacagtac tggcgatcgt cgctccatga
     1261 gaagctgaaa tcatacggtt taccgagata acgttttgcc gtttgcgcca gtttttgctg
     1321 ttgttcaaca ctcagtccgc cttcaacgcg gcgaacaacg tatttgccct tttcaccatg
     1381 ggcgatccac tgcttgagcg gggtgtattt caccgggccg accgcttcaa aaacgtaggg
     1441 ctttttgttg cgtatcacca gcataccggt gtggctataa tcggaatggg tcgccagttg
     1501 gatcgcttta ctttgcgatg agcgagagat ctgaaagatg atgtcgccgg tttgtggctg
     1561 ccaggcaaag gcaggtacag aaaccagcag gctgaggatc agcagcctgt ttttcatagt
     1621 taaacgtcca tgtataaaaa gcggtgggtc gcagacaacg tgctcgttgt ttatgccgga
     1681 tgcggcgtga acgccttatc cggcctacaa gttcgtgcaa attcaataaa ttgcaatatg
     1741 acgtaggcct gataagcgta gcgcatcagg cgattccact ccgcgccgct cttttttgct
     1801 ttagtattcc cacgtctccg ggttaatccc catctcacgc atgatctctt ttgccgcttc
     1861 cgggatttcg tcgctgcgct ctttacgcag atcggcatca tccggcaaag gttgcccggt
     1921 aaaggcatgc agaaacgctt cgcacagcag ctcgctgttg gtagcgtgac gcaggttgtt
     1981 cacctgacga cgcgtgcgtt catcggtgag gatttttaac accttaagag gaatggaaac
     2041 cgtaatcttt ttgacttgtt cactcttctt gccgtgctca gcgtatgggc tgatatattc
     2101 gccgctccat tcagccatga gatacttaat cctcttcgtc aataaattga gaccagacca
     2161 cacaatttat gtgggtactg accgtaaacc cgcatagttt accgtacagg cgttaccgtg
     2221 acatcgtgta atgcacctgt cggcgtgata atgcatataa ttttaacggc tatttgggat
     2281 ttgctcaatc tatacgcaaa gaagtttaga tgtccagatg tattgacgtc cattaacaca
     2341 atgtttactc tggtgcctga catttcaccg acaaagccca ggaaacttca tcacatgacg
     2401 cgtaaacagg ccaccatcgc agtgcgtagc gggttaaatg acgacgaaca gtatggttgc
     2461 gttgtccctc ccatccatct ttccagcacc tataacttta ccggatttaa tgaaccgcgc
     2521 gcgcatgatt actcgcgtcg cggcaaccca acgcgcgatg tggttcagcg tgcgctggca
     2581 gaactggaag gcggtgctgg cgcagtactt actaataccg gcatgtccgc gattcacctg
     2641 gtaacgaccg tctttttgaa acctggcgat ctgctggttg cgccgcacga ctgctacggc
     2701 ggtagctatc gcctgttcga cagtctggcg aaacgcggtt gctatcgcgt gttgtttgtt
     2761 gatcaaggcg atgaacaggc attacgggca gcgctggcag aaaaacccaa actggtactg
     2821 gtagaaagcc caagtaatcc attgttacgc gtcgtggata ttgcgaaaat ctgccatctg
     2881 gcaagggaag tcggggcggt gagcgtggtg gataacacct tcttaagccc ggcattacaa
     2941 aatccgctgg cattaggtgc cgatctggtg ttgcattcat gcacgaaata tctgaacggt
     3001 cactctgacg tggtggctgg cgtggtgatt gctaaagacc cggacgttgt cactgaactt
     3061 gcctggtggg caaacaatat tggcgtgacg ggcggcgcgt ttgacagcta tctgctgcta
     3121 cgcgggttgc gaacgctggt gccgcgtatg gagctggcgc agcgcaacgc gcaggcgatt
     3181 gtgaaatatc tgcaaaccca gccgttggtg aaaaaactgt atcacccgtc gctgccggaa
     3241 aatcaggggc atgaaattgc cgcgcgtcag caaaaaggct ttggcgcaat gttgagtttt
     3301 gaactggatg gcgatgagca gacgctgcgt cgtttcctga gcgggctgtc gttgtttacg
     3361 ctggcggaat cattaggggg agtggaaagt ttaatctctc acgccgcaac catgacacat
     3421 gcaggcatgg caccagaagc gcgtgctgcc gtcgggatct ccgagacgct gctgcgtatc
     3481 tccaccggta ttgaagatgg cgaagattta attgccgacc tggaaaatgg cttccgggct
     3541 gcaaacaagg ggtaaaaatg agtgtgattg cgcaggcagg ggcgaaaggt cgtcagctgc
     3601 ataaatttgg tggcagtagt ctggctgatg tgaagtgtta tttgcgtgtc gcgggcatta
     3661 tggcggagta ctctcagcct gacgatatga tggtggtttc cgccgccggt agcaccacta
     3721 accagttgat taactggttg aaactaagcc agaccgatcg tctctctgcg catcaggttc
     3781 aacaaacgct gcgtcgctat cagtgcgatc tgattagcgg tctgctaccc gctgaagaag
     3841 ccgatagcct cattagcgct tttgtcagcg atctggaacg cctggcggcg ctgctcgaca
     3901 gcggtattaa cgacgcagtg tatgcggaag tggtgggcca cggggaagta tggtcggcac
     3961 gcctgatgtc tgcggtactt aatcaacaag ggctgccagc ggcctggctt gatgcccgcg
     4021 agtttttacg cgctgaacgc gccgcacaac cgcaggttga tgaagggctt tcttacccgt
     4081 tgctgcaaca gctactggtg caacatccgg gcaaacgtct ggtggtgacc gggtttatca
     4141 gccgtaacaa cgccggtgaa acggtgctgc tggggcgtaa cggttccgac tattccgcga
     4201 cacaaatcgg tgcgctggcg ggtgtttctc gtgtaaccat ctggagcgac gtcgccgggg
     4261 tatacagtgc cgacccgcgt aaagtgaaag atgcctgtct gctgccgttg ctgcgtctgg
     4321 atgaagccag cgaactggct cgtctggcgg ctcccgttct tcacgcccgt actttacagc
     4381 cggtttctgg cagcgaaatc gacctgcaac tgcgctgtag ctacacgccg gatcaaggtt
     4441 ccacgcgcat tgaacgcgtg ctggcctccg gtactggtgc gcgtattgtc accagccacg
     4501 atgatgtctg tttgattgag tttcaggtgc ccgccagtca ggatttcaaa ctggcgcata
     4561 aagagatcga ccaaatcctg aaacgcgcgc aggtacgccc gctggcggtt ggcgtacata
     4621 acgatcgcca gttgctgcaa ttttgctaca cctcagaagt ggccgacagt gcgctgaaaa
     4681 tcctcgacga agcgggatta cctggcgaac tgcgcctgcg tcaggggctg gcgctggtgg
     4741 cgatggtcgg tgcaggcgtc acccgtaacc cgctgcattg ccaccgcttc tggcagcaac
     4801 tgaaaggcca gccggtcgaa tttacctggc agtccgatga cggcatcagc ctggtggcag
     4861 tactgcgcac cggcccgacc gaaagcctga ttcaggggct gcatcagtcc gtcttccgcg
     4921 cagaaaaacg catcggcctg gtattgttcg gtaagggcaa tatcggttcc cgttggctgg
     4981 aactgttcgc ccgtgagcag agcacgcttt cggcacgtac cgactttgaa tttgtgctgg
     5041 caggcgtggt ggacagccgc cgcagtctgt tgagctatga cgggctggac gccagccgcg
     5101 cgttagcctt cttcaacgat gaagcggttg agcaggatga agagtcgttg ttcctgtgga
     5161 tgcgcgccca tccgtatgat gatttagtgg tgctggacgt taccgccagc cagcagcttg
     5221 ctgatcagta tcttgatttc gccagccacg gtttccacgt catcagcgcc aacaaactgg
     5281 cgggagccag cgacagcaat aaatatcgcc agatccacga cgccttcgaa aaaaccgggc
     5341 gtcactggct gtacaatgcc accgtcggtg cgggcttacc gatcaaccac accgtgcgcg
     5401 atctgatcga cagcggcgat actattttgt cgatcagcgg gatcttctcc ggcacgctct
     5461 cctggttgtt cctgcaattc gacggtagcg tgccgtttac cgagctggtg gatcaggcgt
     5521 ggcagcaggg cttaaccgaa cctgacccgc gtgacgatct ctctggcaaa gacgtgatgc
     5581 gcaagctggt gattctggcg cgtgaagcag gttacaacat cgaaccggat caggtacgcg
     5641 tggagtcgct ggtgcctgcc cattgcgaag gcggcagcat cgaccatttc tttgaaaatg
     5701 gcgatgaact taacgagcag atggtgcaac ggctggaagc ggcccgcgaa atggggctgg
     5761 tgctgcgcta cgtggcgcgt ttcgatgcca atggcaaagc gcgtgtgggc gtggaagcgg
     5821 tgcgtgaaga ccatccgctg gcatcactgc tgccgtgcga taacgtcttt gccatcgaaa
     5881 gccgctggta tcgcgataat ccgctggtga tccgtgggcc tggcgctggg cgcgacgtca
     5941 ccgccggggc gattcagtcg gatatcaacc ggctggcaca gttgttgtaa tttcagaaat
     6001 ttaataatgc ccggtactca tgttttcggg tttatggttt ctaatgaaat atattgaatt
     6061 atcataggat taggccggat taagcgttta cgacgaatcc ggcaagaagc aataagtaca
     6121 tggttagttt atatttgcag tccggtttgc tttgcatacc ggattttctt tttcttacca
     6181 tcctgaagtt ttttcatctt ccctgatttt tcctcaccat cattggtcat ttttcggttg
     6241 acgcccttcg gcttttcctt catctttaca tctggacgtc taaacggata gatgtgcaca
     6301 acacaacata taactacaag cgattgatga ggtaaggtat gagctttttt cacgccagcc
     6361 agcgggatgc cctgaatcag agcctggcag aagtccaggg gcagattaac gtttcgttcg
     6421 agtttttccc gccgcgtacc agtgaaatgg agcagaccct gtggaactcc atcgatcgcc
     6481 ttagcagcct gaaaccgaag tttgtatcgg tgacctatgg cgcgaactcc ggcgagcgcg
     6541 accgtacgca cagcattatt aaaggcatta aagatcgcac tggtctggaa gcggcaccgc
     6601 atcttacttg cattgatgcg acgcccgacg agttgcgcac cattgcacgc gactactgga
     6661 ataacggtat tcgtcatatc gtggcgctgc gtggcgatct gccgccggga agtggtaagc
     6721 cagaaatgta tgcttctgac ctggtgacgc tattaaaaga agtggcagat ttcgatatct
     6781 ccgtggcggc gtatccggaa gttcacccgg aagcaaaaag cgctcaggcg gatttgctta
     6841 atctgaaacg caaagtcgat gccggagcca accgcgcgat tactcagttc ttcttcgatg
     6901 tcgaaagcta cctgcgtttt cgtgaccgct gtgtatcggc gggcattgat gtggaaatta
     6961 ttccgggaat tttgccggta tctaacttta aacaggcgaa gaaatttgcc gatatgacca
     7021 acgtgcgtat tccggcgtgg atggcgcaaa tgttcgacgg cctggatgat gatgccgaaa
     7081 cccgcaaact ggtgggcgcg aatattgcca tggatatggt gaagatttta agccgtgaag
     7141 gagtgaaaga tttccacttc tatacgctta accgcgctga aatgagttac gcgatttgcc
     7201 atacgctggg tgttcgacct ggtttataaa tagtgtggct tttgtgaaaa tcacacagtg
     7261 atcacaaatt ttaaacagag cacaaaatgc tgcctcgaaa tgagggcggg aaaataaggt
     7321 tgtcagcctt gttttctccc tcattacttg aaggatatga agctaaaacc cttttttata
     7381 aagcatttgt ccgaattcgg acataatcaa aaaagcttaa ttacgatcaa tttgacctac
     7441 atctctttaa ccaacaatat gtaagatctc aactatcgca tccgtggatt aattcaatta
     7501 taacttctct ctaacgctgt gtatcgtaac ggtaacactg tagaggggag cacattgatg
     7561 agcacgtcag acgatatcca taacacgaca gccactggca aatgcccgtt ccatcagggc
     7621 ggtcacgacc agagcgcggg ggcgggcaca accactcgcg actggtggcc aaatcaactc
     7681 cgtgttgacc tgttaaacca acattctaat cgttctaacc cgctgggtga ggactttgac
     7741 taccgcaaag aattcagcaa attagattac tacggcctga aaaaagatct gaaagccctg
     7801 ctgacagaat ctcagccgtg gtggccagcc gactggggca gctacgccgg tctgtttatt
     7861 cgtatggcct ggcacggcgc aggtacttac cgttcaatcg atggacgcgg cggcgcgggt
     7921 cgtggtcagc aacgttttgc accgctgaac tcctggccgg ataacgtaag cctcgataaa
     7981 gcgcgtcgcc tgttgtggcc aatcaaacag aaatatggtc agaaaatctc ctgggccgac
     8041 ctgtttatcc tcgcaggtaa cgtggcgctg gaaaactccg gcttccgtac cttcggtttt
     8101 ggtgccggtc gtgaagacgt ctgggaaccg gatctggatg ttaactgggg tgatgaaaaa
     8161 gcctggctga ctcaccgtca tccggaagcg ctggcgaaag caccgctggg tgcaaccgag
     8221 atgggcctga tttacgttaa cccggaaggc ccggatcaca gcggcgaacc gctttctgcg
     8281 gcagcagcta tccgcgcgac cttcggcaac atgggcatga acgacgaaga aaccgtggcg
     8341 ctgattgcgg gtggtcatac gctgggtaaa acccacggtg ccggtccgac atcaaacgta
     8401 ggtcctgatc cagaagctgc accgattgaa gaacaaggtt taggctgggc gagcacttac
     8461 ggcagcggcg ttggcgcaga tgccattacc tctggtctgg aagttgtctg gacccagacg
     8521 ccgacccagt ggagcaacta tttcttcgaa aacctgttca aatatgagtg ggtacagacc
     8581 cgcagtccgg ctggcgcaat ccagttcgaa gcggtagacg caccggaaat tatcccggat
     8641 ccattcgatc cgtcgaagaa acgtaaaccg acgatgctgg tgaccgacct gacgctgcgt
     8701 tttgatcctg agttcgagaa gatctctcgt cgtttcctca acgatccgca ggcgttcaac
     8761 gaagcctttg cccgtgcctg gttcaaactg acgcacaggg atatggggcc gaaatctcgc
     8821 tacatcgggc cggaagtgcc gaaagaagat ctgatctggc aagatccgct gccgcagccg
     8881 atctacaacc cgaccgagca ggacattatc gatctgaaat tcgcgattgc ggattctggt
     8941 ctgtctgtta gtgagcgggt atcggtggcc tgggcatctg cttctacctt ccgtggtggc
     9001 gacaaacgcg gtggtgccaa cggtgcgcgt ctggcattaa tgccgcagcg cgactgggat
     9061 gtgaacgccg cagccgttcg tgctctgcct gttctggaga aaatccagaa agagtctggt
     9121 aaagcctcgc tggcggatat catcgtgctg gctggtgtgg ttggtgttga gaaagccgca
     9181 agcgccgcag gtttgagcat tcatgtaccg tttgcgccgg gccgcgttga tgcgcgtcag
     9241 gatcagactg acattgagat gttcgagctg ctggagccaa ttgctgacgg tttccgtaac
     9301 tatcgcgctc gtctggatgt ttccaccacc gagtcactgc tgatcgacaa agcccagcaa
     9361 ctgaccctga ccgcgccgga aatgaccgcg ctggtgggcg ggatgcgcgt actgggtgcc
     9421 aacttcgatg gcagcaaaaa cggcgtcttc actgaccgcg ttggcgtatt gagcaatgac
     9481 ttcttcgtga acttgctgga tatgcgttac gagtggaaag cgaccgacga atcgaaagag
     9541 ctgtttgaag gccgtgaccg cgaaaccggc gaagtgaaat acactgccag ccgtgcggat
     9601 ctggtatttg gttctaactc tgtcctgcgt gcggtggctg aagtttacgc cagcagcgat
     9661 gctcacgaga agtttgttaa agacttcgtg gcggcatggg tgaaagtgat gaacctcgac
     9721 cgtttcgacc tgctgtaatc tgaccttctt cagcgactgc ctttcaggca gtcgctgaag
     9781 tttctttacc ggcgtatagt gtccacagga aaactacaca ctggatctct catgtctgcc
     9841 gcaggaaaga gcaacccact ggcaatcagt ggcctggttg tgctcacact tatctggagt
     9901 tatagctgga ttttcatgaa gcaagtcacc agctacatcg gtgccttcga ctttaccgcc
     9961 ttacgctgca ttttcggcgc tctcgtttta ttcatcgtcc ttttattacg tggtcgcgga
    10021 atgcgcccga caccgtttaa atacacctta gccattgccc tgttacaaac ctgcgggatg
    10081 gttggcctgg cgcagtgggc gctggtcagc ggcggagcgg ggaaggtggc gatcctaagc
    10141 tataccatgc cgttctgggt ggtgattttc gccgcgttgt ttctcggtga acgcctgcga
    10201 cgtgggcaat atttcgcgat actgattgcc gctttcggct tatttttggt gttgcagccg
    10261 tggcaactcg atttctcttc gatgaaaagt gccatgctgg caatcctctc cggcgtcagt
    10321 tggggggcga gcgcgattgt tgctaaacgt ctgtatgccc gtcatccgcg cgtggattta
    10381 ttgtcgttaa catcctggca gatgctctat gcggcgctgg tgatgagtgt ggtcgcttta
    10441 ctggtgccgc aacgtgaaat tgactggcag cccaccgtgt tctgggcgct ggcctacagt
    10501 gcgattctgg cgacggcact ggcgtggagc ttatggttgt ttgtattgaa aaacttgcct
    10561 gccagtattg ccagcttaag cacactggcc gttcccgttt gcggcgtact cttttcctgg
    10621 tggctgctcg gcgagaatcc gggggccgtt gaaggtagcg gtattgtgct gattgtgatg
    10681 gcactggcgc tggtgagccg taagaaaaaa gaagccgtta gtgtaaaaag gatctgaatt
    10741 ttttcttcat gtggggcgat cgcttattta acaaaataac gataatgccc caccatgcgc
    10801 cagctaaaca gcacatcttc ttcctgcgca cctgcgccaa tgttatgtat caccagcggc
    10861 gtaccgtcgc gggcgaagcc atctgaaacc accccaatat gtgccagccc gttatctaat
    10921 cgccaggaga caatatcgcc cgcttgatag tcactggggt tcttgctgat ggggcgtgtt
    10981 ttatcgtggt gggtaaacca ggtttccaga ttaggcaccc gacggtgatc gatgttgctg
    11041 tccgggcgct ttaactgcca tttttgcggg tactcggcaa aattcttcgc catatcttcg
    11101 tgcaccagtt tctgcaaatc gaccttctgg ctgcgcaatg cacggatcac cacatcggaa
    11161 cataccccgc gtttttgcgg gacatcaccg ccaggatagg taagctgcat atacgccgga
    11221 tcgtaaaata gcgtgctgcc gatttgctgt ctggcaccgt ctgcgatggc aaggttggta
    11281 ttggcctgga tttgtaccac ggttggtgga acggcgggag attttaagga gtggctggta
    11341 aatgccgtta gcaggctgag cagcgccagt gaagctttca tctggttcgt ccgtgaaaat
    11401 aagagtcaca gattcgacct tccgggataa cgattgttct gtagagaaaa tgtccagaag
    11461 cggataaaaa actcctgaac aacgtgggag tttcagatta ctggcacaga gtgccggatg
    11521 cggcgtgaac gccttatctg tcctaaaaaa caagaaaatt caatatattg cagagattgc
    11581 gtaggcctga taagcgtagc gcatcaggca attttgcgtt caaactcccg gacaagccgg
    11641 gagtttggag taggttattc ccactcttgc aggaaacgct gaccgtactg gtcggctacc
    11701 agcagagcgg cgtaaacctg atctggcgtc gcgccgccag gcatgttgtg gatggtttca
    11761 cattctgcac atgccgcttc agccacaatt cgcattttcg ccgggacatc ttctttaata
    11821 tccagttgag cgagagttat tggcaaacct accgcatggc taagtgcagc tacggtttcg
    11881 atttcctcaa ccggtgcgtt ttccagaacc agctgcgtca gcgtaccaaa tgccactttt
    11941 tcaccgtgat aatagtgatg cgcgtccggg atagcggtca ggccgttatg cactgcgtgc
    12001 gccgcagcca gaccaccact ttcaaaacca acaccgctca aataggtgtt cgcttcaatc
    12061 acgcgctcca gcgccggagt cactacatgc tgttcggcag caagcatcgc tttttcgcct
    12121 tcttccagca gggtgttgta gcacagttca gccagtgcca gcgcagcctg ggtgcacttg
    12181 ccgcccgcca tggtggtcgc gccgctacga gagcaggcac gcgcttcaaa ccaggttgcc
    12241 agcgcatcgc cgatacccgc cgctaacaga cgtgcaggtg cgccagcgac gattttggtg
    12301 tcgacaatga ccatattcgg gttatttggc aacagcagat agcggtcaaa ctcaccctca
    12361 tcggtgtaga taacagacaa tgcgctgcac ggtgcatcgg tagaggcgat agtcggtgcg
    12421 atcgctaccg gaacacccat gaaatgtgcc agtgctttgg ctgtatcgag ggtttttccg
    12481 ccaccgatac cgagaattgc gccacactgc gcagtctccg cgatgccacg cagacggtcg
    12541 atctcatttt gcgaacatcc accgccaaac ggcgcaattt ctactaccag tccagcatct
    12601 ttaaagcttt tctcgacagt ggattgagca aaacctaaaa caaatttgtc acccaccact
    12661 aaccagcgtt ctgccagcgg cttcaggtat tcgcccagac gattaatcac atcagcgccc
    12721 tggatgtatt tacccggtga ttgaataatg cggtccataa ttgctccttt agagatgagt
    12781 agtgccaaat gcggcattcc agtcgtgttc gaacttctct atagctgact ctaccgcagg
    12841 ggtgttgagc atttgttgcg ctacatctaa gggcagggtg atggattcac atcctgccag
    12901 taaacagtcc agcgcctgac gcggcgtttt aaagctggct gccagcacca tgctttctgg
    12961 cgcgtgcatt tctaacagcg cttgcagctc ctgaaccgta cgaatgccgt ctccgccctg
    13021 ggcatctacg cggttaacat acggagcaac gtattttgcc cctgccagcg cggccagtaa
    13081 cccttgtgca gcactgtaca ctgcggtgcc aagcgtagta ataccctctt ttttcagtat
    13141 tttaattgct gccagacctt cggaagtcac cgggattttc accacaatac ccggaatagc
    13201 gtcgcgcagg cgcttcgctt cttccaccat cccctgcgcg tcgcggctca tggtctgagc
    13261 aaacagaatg ccctcatcac caatcgcttt ttgcagacgc ggcagcactt cccatatgga
    13321 ctccttgctg gcagcgataa tgctcgggtt agttgtcacc ccggcaatgg ggaatatgcg
    13381 tgccagacgt tcgacttctg cgacgttagc ggtgtccaga tacagttcca tgatgtttcc
    13441 ttttacagtt ccagttcatg ttgcagcagg ctggcgatag cgtcggcaga ggcggcgtta
    13501 accagcgcgt tacggaattc ttcgtgcatg atgcggcgag cgaggcgcga gaaaatgcgc
    13561 atgtgctgat cgcccgcagc gtgtttgttc agggttaaca tgatgatgaa ttgcgcttca
    13621 tcatcacccc agcgcaccgg cgcttgcaga cgcgcaacgc tgatggtgga ttgctcaatg
    13681 tgttctgatt tgctgtgtgg aatggcaaaa ctaaagccca gaccggtaga gaaaacggcc
    13741 tcgcgcgccc acaagtcggc ttccagttta cgcggatagc ggcagcgccc tgccagcagc
    13801 aggttatcgg tcatgccttt gagcacttct tctttgctgc gccagtcgct ttccagtgtg
    13861 atgcactcgg cggtgaccag cggtgcgtct tgttgggtca tgcggaattg cgccagcagg
    13921 tgctccactt ccagcgaagt acggcaggcc attgcctggt tgagcaactt gcggcactca
    13981 cggctatcaa gttgcgccat ccgcgctttc gccgccggaa ttgatggtgc gctcatgctg
    14041 agttcatcca gccctaagcc gaccagcaac ggcagcacgg aacctttcgc tcccagctca
    14101 ccgcacagac caatccattt accctggcga tgcaccgcct gcacggcgta atcgagcgcc
    14161 cgcaagaatg ccggattcag gctgttgtag tgacgagtaa ccttagcgtt atcgcgatcc
    14221 actgccagca gatattgcgt caggtcatta ctaccaatac taaagaaatc aatctcttcg
    14281 cagcattgat cgatgatgaa catcaccgac ggcacttcca gcataatgcc gagctggatc
    14341 ttctcatcaa acggaatgtg ttcgttacgt agttgctgtt tggcttctgc cagtttttct
    14401 ttcacccata agatctcttc cattgaggag atcatcggga tcatgatttt caggctgccg
    14461 tgagcggagg cgcgcaggat cgagcgtagt tgtgtggtaa ataacgacgc gtattcttca
    14521 taaatacgca cggcgcgata accgaggaac gggtttgcct ctgcgggaat gtttagataa
    14581 tcaacgggtt tgtcgccgcc aatgtccata gtgcgcacaa taatgctgcg tccgttggca
    14641 gactccagcg cctgacaaaa aatgttgtac agctcgcttt cgcccggtgc actggtgcga
    14701 tccatataga gcatttcagt gcggaacaaa ccaacgcctt ccgcaccatt gccgaatgcg
    14761 gcctgcgctt ccacggagtg agcgatgtta gcggcaattt caatgcggat accgtcagcg
    14821 gtacgggctt gttgggtcag ccagacacgc tgttgctcac gcagggcgtc ctgtacgcgg
    14881 gcttcttgct gataataacg agctacggct tctcctggct caaccacaat cgccccggcg
    14941 ttgccgtcga tataaatcgt ttgatgctgc cacggagtga gggcatcaat atccacacca
    15001 accagcgtcg gaatgttgaa cgaacgggca aggatcaccg tatgtgaggt ggtgccgcca
    15061 cttttcagca acagaccttt gaggtgattt ttatccagtt cgaggaactg gctgggggtc
    15121 agttcatcag ccatacaaat ggcggactgc gtcagtttgc ccggtgccgg gaagcgttgc
    15181 tcaccgtaga tttgctggag taactggaag cagacgtcgc gtacgtccag ggcacgttct
    15241 tgcaggtagc tgctgctgga acgggcgaac tcttcgcaaa agtgattcgc actggcgaca
    15301 atcgcttcgg cgcagcttaa tccctcgctg acgcctgcca gtaagtgctg acgtaaagat
    15361 gtatcgccag ccagggatcg gtgagcttcc agtatcgcgc tggtagcacc gtcgctatcc
    15421 agcagacgaa actcaatgtt tttcagtacc agcgtcaggc cgttttccag tgtggattgc
    15481 tcggcgtcaa cgtctttggc tgcgggaaga ttactcagcg cattgagatc taaagaagag
    15541 atcggcgtca gaatgccgcc tgcactaccg ctgcacacgg tgcgggcgcg gataatttgc
    15601 ggattcagat tggtcagtga aaccggcagt ggttccagtt cgtcagattt aacttccgcc
    15661 agcggcgcat cgcagtgggg gaattcatcg cgcagccatt ggcttaaacg ctggtgcgct
    15721 tcctgttcgt cggccccgga aatcagtaac tggcagttat cgcccgccag cgtatcggtg
    15781 ccaatcagcg caagggcgct tttggcgttg cccttgcggt cagtgtgcag gttatgccac
    15841 tcaatttgtg atgaaaaagt attacacagc gtttcaacgt ggcttgccgg acgcgcatgt
    15901 acgccgttag gtagctcaca aataaattcc acaatcaggg ccattgcctc tctcccggta
    15961 cggttttctg tttacaggat aagagcgcac ggcaacggcc tgccatgtga caaatctgcc
    16021 aaaagctgga caaatgtaat gtaaccgtca atttgcgacg cgtctcacaa gacgccgttt
    16081 tgcggcatgc ttccggttta tcgcaagtta tgaggcggat cgcatttttg tactgatatt
    16141 acaaaagtcc agtaaatggc ctttttatcc actgtttgct ccgcctgcga ttgcctattg
    16201 tttcctccat caaaaatatg gacatgggct ttcgcaagcc cgggagcagc ttatgaatga
    16261 gttggtgcag atcctgaaaa atacccgtca gcatttaatg acgggcgttt cacacatgat
    16321 tcccttcgtg gtatcgggcg gtattttgct ggcggtttcc gtcatgttgt atggcaaagg
    16381 cgcagtgccg gatgccgtag ccgatccaaa tctgaaaaaa ctgtttgata tcggcgttgc
    16441 gggcttgacg ctgatggtgc ctttcctcgc cgcttacatt ggttattcca ttgcagaacg
    16501 ttctgcgctg gctccatgcg ctatcggtac ctgggttggt aacagctttg gtgcgggctt
    16561 ctttggtgca ctgatcgccg ggattatcgg cggcatcgtg gtgcattacc tgaagaaaat
    16621 tccggtgcat aaagttctgc gctcggtgat gcctatcttc attattccta tcgtcggcac
    16681 actgattacc gcaggcatca tgatgtgggg cttgggcgag cctgtagggg cgttgaccaa
    16741 cagcctgact cagtggcttc aggggatgca gcagggcagc attgttatgc tggcggtgat
    16801 catgggtctg atgctggcgt tcgatatggg cggtccggtt aacaaagtgg cctatgcctt
    16861 catgctgatt tgcgttgctc agggtgttta taccgtggtg gccatcgctg ccgtgggtat
    16921 ttgtatcccg ccgctgggga tggggctggc gacgctgatt ggtcgtaaaa atttctccgc
    16981 agaagagcgc gaaaccggca aagcggcact ggtgatgggc tgtgttggcg ttactgaagg
    17041 ggcgattcct ttcgccgctg ccgatccgct gcgtgtcatt ccttccatca tggtcggttc
    17101 agtttgtggt gcagtaactg cggcgctggt cggtgcgcag tgctatgcag gctggggtgg
    17161 tctgattgtg ctgccggtgg ttgaaggcaa gctgggttat atcgcagcag tggctgtcgg
    17221 agcagtggtg acggctgttt gtgtgaacgt gctgaaaagt ctggcgcgta aaaatgggtc
    17281 ttcgactgat gaaaaagaag acgacctgga tttggatttt gaaattaatt aattgaggaa
    17341 cgagccatga cgaaaattat tgcagtaacc gcatgtcctt caggtgttgc ccatacctat
    17401 atggcagcag aggcgctgga aagtgccgcg aaagcgaaag gctgggaagt gaaagtagaa
    17461 acctaggggt caattggtct ggaaaatgaa ctgactgcgg aagatgtggc gagcgctgat
    17521 atggtgattc tgaccaaaga tatcggcatc aagtttgaag agcgttttgc tggcaagacc
    17581 atcgtgcgcg tcaacatcag cgatgcagta aaacgtgctg acgccattat gagcaaaatt
    17641 gaagctcacc tggcgcaaac cgcttaagtt cttctcccgc tcgcaagggc gggttcgctt
    17701 tcccacagga gttcctcatg acgaatcgta tctctcgcct caaaactgca ctgtttgcca
    17761 atacccgtga aatctcgctg gagcgggcgc tgctttatac cgccagccat cggcaaaccg
    17821 aaggcgaacc ggtgatattg cgccgggcga aagcaacagc gtatatcctt gaacatgttg
    17881 aaatttcgat tcgtgatgaa gaactgattg ccggtaaccg caccgtaaaa ccgcgcgccg
    17941 ggattatgtc gccggaaatg gacccttact ggctgctgaa agagctggat caattcccga
    18001 cgcgtccgca ggaccgcttt gctatcagcg aagaagataa acgtatctac cgtgaagagt
    18061 tgttcccgta ctgggaaaaa cgttcgatga aagatttcat caacgggcag atgacagatg
    18121 aagtaaaagc cgcgaccaac acgcagattt tcagcatcaa ccagacggat aaaggccagg
    18181 ggcacattat tattgattac ccacgcctgt tgaatcacgg tctgggtgag ctggtggcac
    18241 agatgcagca acattgtcag caacagccgg agaatcactt ttatcaggcc gcgttgttac
    18301 tgctggaagc ctcgcagaaa cacattttgc gttacgccga actggcggaa acgatggcgg
    18361 caaactgcac agatgcacag cgtcgcgaag aactgctgac tattgcggag atctctcgcc
    18421 ataacgccga acataagccg cagacgttct ggcaggcgtg ccagttattc tggtacatga
    18481 acatcattct gcaatacgaa tccaacgcca gttcgctgtc tttagggcgc ttcgaccagt
    18541 atatgttgcc gttctatcag gcatctttaa cccagggcga agatccggcg ttcctgaaag
    18601 aactgctcga atctttatgg gtgaaatgca acgacatcgt gctgttgcgc tccaccagca
    18661 gcgcgcgtta tttcgcaggt ttcccgaccg gctataccgc actgctcggc gggttaaccg
    18721 agaacggacg tagcgcggtg aacgtgcttt cgttcctttg ccttgacgcc tatcaaagcg
    18781 tgcaattacc gcaaccgaac ctcggcgtgc gcactaacgc cttgatcgac acgccgttcc
    18841 tgatgaaaac cgccgaaacc attcgcctcg gcaccggtat tccgcaaatc tttaacgatg
    18901 aagtggtggt accagcgttc ctcaaccgtg gcgtttcgct ggaagatgcg cgcgactatt
    18961 ccgtagtggg ctgtgtggaa ttatctattc ccggcagaac ctacggcttg catgacatcg
    19021 cgatgtttaa tctgctgaaa gtgatggaaa tctgcctgca tgaaaatgaa ggcaatgctg
    19081 cgctgactta tgaaggttta ctggagcaga tccgcgccaa gatcagccac tacatcaccc
    19141 tgatggttga gggcagcaat atttgtgata tcggccatcg cgactgggca cctgtaccgc
    19201 tgctctcgtc ttttatcagc gattgtctgg aaaaaggtcg cgatattacc gatggcggcg
    19261 cgcgttataa cttctccggc gtacagggga tcggtatcgc caacctgagc gattctctcc
    19321 atgcgttgaa agggatggtt tttgagcaac agcgtttaag ttttgacgaa ttgctgtcgg
    19381 tattaaaagc caactttgca acgccagaag gcgaaaaagt ccgtgctcgc ttaattaacc
    19441 gcttcgagaa atacggtaac gatatcgacg aggtggataa catcagcgcc gaactgttac
    19501 gccactactg caaagaagtg gaaaaatacc agaacccgcg cgcggttact tcacgccggg
    19561 atcgtatacc gtttctgctc acgtcccgtt gggatcggtg gttggcgcga cgccagacgg
    19621 tcgttttgcc ggggaacaac tggcagacgg cggcttgtca cctatgctgg gccaggacgc
    19681 acaagggcca acagcggtac tgaagtcagt cagtaagctc gataatacgc tgctgtctaa
    19741 cggtacgttg ctgaacgtga aattcactcc ggcgaccctg gaaggtgaag cgggattacg
    19801 caaactggcc gacttcttac gggcgtttac ccagcttaag ttgcagcata ttcagtttaa
    19861 cgtggtgaac gccgacacgt tgcgggaagc gcaacagcgc ccacaagatt atgccgggct
    19921 ggtggtgcgc gttgccggat acagcgcctt ctttgtcgaa ctgtcgaagg agatccagga
    19981 tgacatcatc cgccggacag cgcatcagct gtaacgttgt ggaaacgcgc cgcgatgatg
    20041 tggcgcgcat tttcaacatt cagcgttatt cactgaatga cggcgagggc attcgtacgg
    20101 tggtcttttt taaaggctgt ccgcatcttt gcccgtggtg tgctaatccg gagtcgatct
    20161 ccggcaaaat ccagacggta cgcagagagg cgaaatgtct gcactgtgcg aaatgtttgc
    20221 gtgatgcgga tgaatgcccc tccggggcgt ttgaacggat tggtcgcgat atcagccttg
    20281 acgctctgga acgggaagtg atgaaagatg acattttctt tcgcacgtcc ggcggcggcg
    20341 tcacgctttc tggcggcgaa gtgttaatgc aggcggagtt tgctacccgt tttttacagc
    20401 gactgcggct gtggggtgtg tcatgcgcca ttgaaactgc cggagacgcg ccagcaagca
    20461 agctgttacc gctggcgaaa ttgtgcgatg aagtgctgtt cgatttaaaa attatggacg
    20521 cgactcaggc gcgggatgtg gtgaagatga acctgccacg cgtgctggag aatctgcgtt
    20581 tgctggtgag tgagggtgtc aacgtgatcc cgcgtttacc gttgatccct ggtttcacgc
    20641 tcagcaggga gaatatgcag caggcgctgg atgtactgat cccgctgaaa atcaggcaga
    20701 tccatctgtt accgtttcat cagtacggcg aaccgaaata ccgcctgctg gggaaaacat
    20761 ggtcgatgaa agaggtgcct gcgccgtcgt cagccgatgt gacaacgatg cgcgaaatgg
    20821 cagaacgggc cggatttcag gttaccgtgg gaggttaaaa tggcatacct ggtggcagta
    20881 accgcctgcg taagcggcgt ggcgcatact tatatggcgg cggaacggct ggaaaagttg
    20941 tgccagttag agaagtgggg cgtcagcatt gaaactcagg gcgcgctggg aacggagaat
    21001 cgtttagcgg acgaggatat ccgtcgggcg gatgttgctc tgttgattac ggatatcgag
    21061 cttgccggtg ccgagcgatt tgaacattgc cgctatgtgc aatgcagcat ctacgcattc
    21121 ctgcgtgagc cgcagcgggt aatgagcgcg gtgcgcaaag tgctttctgc gccgcagcaa
    21181 acccatctta ttctggagta gtcggttttt ctgtcagttg gctgtggtac tgccggcgat
    21241 attccgacgg cgagcgttcg gtgtttttac gaaacagacg gcagaagtag ttgctgtcga
    21301 caaagccgca ggcgtgcgcc acttctttta ccttcaggtc atagcctttc aataacgtct
    21361 tagcgtgctc cagtcgcgtg tgattcaggt attcgttaaa accaatggcc cccgtttttt
    21421 gaaacaggtg cgagagatag tttggtgaaa tatagaatgc ctgtgcgaca gattcgcggg
    21481 taagcgcgga ggcatagcgt tcgtcgatat aatcgcgaat agcttcaaat agtgcctggc
    21541 tgcgtgaggc ggtctggatt tggctgccga gcaaatcgcg gcagtggcta agcaggctgg
    21601 ctacgataag ccttgccgtt tgctgctcct gcggctgcat ttgcatttca ttgagcgttt
    21661 gtagcagaaa agaacctata cgtgggccgc gtcgggcgac gtgttgcttc gccagatttt
    21721 gatattgttt gccatcccat tgcacgacgc tgaagccgag ttgctgtttg ccaaacagca
    21781 cgctaaaggt ggtagcgggt gttttccatt gtgggaaatt ccagccacca gcggcaacgt
    21841 acagcacatc gccgggaact aacgttgctc cagcgccggt atcaacaaac tcaccttcca
    21901 gcacaatttc cagccgtgga aaatcgacct gatacgcaag atcgggcacc gggccgttac
    21961 tgctggcaaa gtaaatctgg cgcagggaca gcgggccgtt gatcaggcgg gagagcagat
    22021 agctgacgtc gtgatacatg ttattccctt aggtatgtag acgggaaaac agtaactggc
    22081 attattaaaa aaggccacct gaaaagtgtg gcctgagggg agttcgatgt aaacattgcg
    22141 gcagttgtat tgccggatgt ggcgtaaacg ccttatccag cctaccgccg cgatctgtag
    22201 gccggataag acgcgtcaag cgtcgcatcc ggcaaatagc gcctggctga taattactga
    22261 ttacctacct ggtcgccata cggcagagtg tcgtaatcga tcagcgcgtt tttcttgtac
    22321 gggttaccaa tccagcgggt agtttctttg aactgcggat tcaccactga acgggttgga
    22381 tcgtaaccgt cgtaagataa gcccgccaaa tctgaccagg tgtggatcag ttccgccagg
    22441 ctgtatttac gatcgacatc ctgtgagaaa tcacggggat gagtcgcttg ccatttttcc
    22501 gacgtccaca gcaggaacgg aatggtgtac atgtggcgcg tcgggttgtc ttcgttacgc
    22561 ccctgagttt tgtgcggcgg cgtgtcgtaa acctcttcac cgtggtcaga gaagtaaacc
    22621 aggaatccgt tcgggtctgc cgctttaaag tctttaatca ggctggcaac cacatgatcg
    22681 ttatacaggt tagcgttgtc ataatcgtta tatgattcca gctcttctgc gcttaatcct
    22741 ggcggaacat gatcggtatt gccatcaaac ttgccctgat tttccgggta gcggtatttg
    22801 tatttgatat gcgtacccag cagatgaacg atgatcagtt tcttcggcgc agggtcattc
    22861 agcacatcct ggaacggctt cagcacgttg gtgtcgtatt cacgcgcact ctgcgtacgt
    22921 tgctggttca tgtagtactg cttgtcggtc tggcgcgaaa ataccgtcag catggtattg
    22981 cgggcggtca tcgtctgctg gttggtgatc cagaaggttt tataacccgc ctgtttcatc
    23041 atgttcatca gcgacggctg cgtcagatac agatccgggt tcttttcatt ggcaaaggtc
    23101 agcgcctgtt gcaggatttc aatggtgtac ggacgagacg taactacgtt attaaacacg
    23161 gtcagattcg gatcggtttt atgcagcgca tccagctccg gcgtggtttc acgcggataa
    23221 ccgtacagac tcatgcgtcc gcgctgggtc gactcgccaa tcaccagcac taaagtgcgc
    23281 ggttcgttac ccgattcatc tttgaaatta gccagtggcg gcaaggcatt attttcattc
    23341 agtaactttg ttagcgagtt tagttgctga cgatactgat aatagccggt caggaattgc
    23401 cacggtgcgg caggctccat acgcgaggcc aggttatcca acgttttctc aaacggcttg
    23461 tttttgataa acgtattcat ggcgatcgga tgcagaatca agccataaag cagggcaaaa
    23521 gagacaacat aacgccacgg ctttggaata tagaccgggc gcaggcgtgt ccacagcaga
    23581 actgccaccg ccgtataggc cagcgcgata agcacaattt tcaggctgaa atactggctt
    23641 aaatactcgc tggcttcgtt ggtgttggtt tcgaacatca caaacagaac gctctgcgag
    23701 aactcctgcc cgtagatgac gtagtagcat agcgccgcca gagaggccgc ccatagcacc
    23761 acgccgatta ctgcggcaat aattttaatc cgcttcggaa agaggaatac cgggatcaac
    23821 cacagcgagc tgaataacag cgagtcgcga atgccgttgg tgccactata accactgatg
    23881 taaataatgg cctgtagcag agtagagaaa aaccaaaagt agagcagtgc ccaacccagg
    23941 gctttccagc taaaaagggg tttagcctgg acttctgtgg aatgcatagt aagaacctgt
    24001 cttgaaaaaa atatcgccga atgtaacgac aattccttaa ggatatctga aggtatattc
    24061 agaatttgaa taaaatgcag acagaaatat attgaaaacg agggtgttag aacagaagta
    24121 tttcagaaaa ccctcgcgca aaagcacgag ggtttgcaga agaggaagat tagccggtat
    24181 tacgcatacc tgccgcaatc ccggcaatag tgaccattaa cgcttgttcg acgcgaggat
    24241 ccggttcctg gccttctttt tctgcctggc gggagcggtg cagcaactcg gcctgcaata
    24301 cgttcagcgg gtcggtgtaa atattccgta gctgaataga ctctgcaatc cacggcagat
    24361 cggccatcag atgggaatcg ttggcaatcg ccagcaccac tttgatgtct tcttcttgca
    24421 ggttgcgtaa ctctttacct aacggccaca gtgctttgtc taccagacgt tgatcatagt
    24481 attccgccaa ccacaggtct gctttggcga agaccatctc cagcatgccg agacgcgtcg
    24541 agaagaatgg ccaatcgcgg cacatggctt ccagctcgct ctgtttgcca tcttcgacca
    24601 ctttttgcag cgccgtacct gcacccagcc aggcggggag catcaggcgg ttttgcgtcc
    24661 aggcaaaaat ccacggtatg gcgcgcagcg actcgacgcc gccggttggg cgacgtttcg
    24721 ccggacgtga acccaacggc agtttgccca attcttgttc cggcgtagcg gagcggaagt
    24781 aaggcacaaa atctttgttt tcacgtacgt agccacggta gagatcgcag gagatgactg
    24841 acagttcatc cataatgcga cgccagctct ctttcggctc aggtggtggc agcaggttgg
    24901 cttccagaat cgccccggtg taaagcgaca ggctgctgac ggtgatttct ggcagaccat
    24961 atttaaagcg gatcatctcg ccctgttcag tcacgcgcag gccacctttc aggcttcctg
    25021 gcggttgtga cagcagcgcc gcatgggcag gtgcgccgcc gcgaccaatg gaaccgccgc
    25081 gaccgtggaa caacgtcagc tcaatacccg ctttttcgca ggttttgatt aatgcatcct
    25141 gtgcctgata ttgcgcccag gaagctgcca tcacgcccgc atcttttgct gagtcggaat
    25201 agccaatcat caccatctgt ttgccctgaa tcaggccgcg ataccagtcg atattgagca
    25261 gctgggtcat gacatcgttg gcgttgttca ggtcatcgag ggtttcaaac agcggagcaa
    25321 ccggcatcgc aaacccgata cccgcttctt tcagcagcag gtggacagcc agtacgtcgg
    25381 acggcgtttt cgccatcgag atcacgtagg cggcaatgga gccttgcggc gcttcggcaa
    25441 tcacctggca ggtatcgagc acttcgcgcg tttcggcgct tggttgccag ttgcgcggca
    25501 gaagcggacg tttggagttc agttcgcgga tcaggaacgc ctgtttgtcg gcctctgacc
    25561 agctttcgta gtcgccgata ccgaggtagc gggtcagctc gcccagcgct tcggtatggc
    25621 gggtgctctc ctgacggata tcaatacgga ccagcggtac gccgaaacat ttcacgcggc
    25681 gcagggtgtc gagcagatcg ccgttggcga taatacccat gccacacgcc tgaagtgact
    25741 ggtagcaagc gtagagcggt tcccacagtt cttcgttttg tgtcagcagc ccttctggtt
    25801 ttggcagttc ttcgcctttc aggcgcgctt ccagccatgc ctgtgtcgcc atcaggcgag
    25861 aacgcaggtt tttcatcaga tagcgatacg gttctgcggc accttcttcg ccaaccagcg
    25921 ccagcagttc aggggtcgct tcaaccatcg acagttcaga aaccagaacc tgaatatctt
    25981 tcaggaacag atcggtggct ttccagcgac tgagtagcag gacgtggcgg gtgatatcgg
    26041 cagtgacgtt cgggttgccg tcgcggtcac cgcccatcca cgaagtaaaa cggaccggaa
    26101 caaattcgac gggcagtttg tagccgaggt tctcttccag ttgttcgttc agttcgcgca
    26161 agtaattcgg tacgccttgc cacaggctgt tttctactac ggcaaagccc catttggctt
    26221 catctaccgg gcttggacgc agcttacgga tttcatcagt atgccatgac tgggcgatta
    26281 actggcgcag gcgacgcatc agctggttgt gttcgtagtc agcgatatct ttgttatcga
    26341 gctgttttaa gcaggcgttc acttccacca ttttgtggat cagtgtacga cgggtaattt
    26401 cggttgggtg agccgtgagg accagttcca gcgacagcga ttccactgct tttttgatgg
    26461 tgtcttcgct cagttccggc tggtttttca gtttgcgcag ggtgcgggcg atcacttccg
    26521 ggttgctggc agcttcgcct ttcggcgaaa tgctgtggta ttgctcggcg gtgttggcta
    26581 ggttcaggaa ctgactaaac gcacgcgcaa cgggcagcag ctcgtcgttc gacaaatttt
    26641 gtaaggtggt gagcaactcc tggcggttag catcattgcc agcgcgtgaa gatttcgaca
    26701 acttacggat agtttctacg cgttcaagaa tgtgttctcc caacgcatcc ttgatggttt
    26761 ctcccagcac tttgccgagc atactgacat tactacgcaa tgcggaatat tgttcgttca
    26821 tattacccca gacaccccat cttatcgttt gatagccctg tatccttcac gtcgcattgg
    26881 cgcgaatatg ctcgggcttt gcttttcgtc gtcttttata aagccacgta aaagcggtga
    26941 cgtcaaatgc tgcgaaatcg cttcagcaaa cgaataaata gcaggaattt acgccattaa
    27001 attcacgacg ctttaaataa gcgtaactta tggaaatgtt aaaaaatcgc catacataac
    27061 accaaaggtg taggtcggat aagatgcgca agcatcgcat ccgacattat tgcggcaccg
    27121 gagtttggca acggtgccgg atgcggcgcg agcgccttat ccggcctacg ttttaatgcc
    27181 agcaaaaatg gtgaattacc tggataatca actcacgggt gggtttaata aaccgcgtct
    27241 ccagatattc atcaggctga tgagcttgat tgatggaacc cggaccgagc accagcgtcg
    27301 ggcataacgt ttgaataaac ggcgcttcgg tacagtaatt caccacttcg gtttttgctc
    27361 cgagcaattt ctcaaccact tcaaccagtt gatgattcgg tgggcattca tagccaggga
    27421 tcggcggatg cagctcgtcg accgtcagac gacccggcca gcgttcgctc accggagcca
    27481 atgcatcgtt gagcaaacca ttaagttcat tgagtgtcat gccaggcagc ggacgaatat
    27541 ccatatgcaa ctcacagcaa gcgcaaatac ggttagaagc gtcgccaccg tgaatatgcc
    27601 cgaggttgag cgtagggtat ggcacggtaa atgcttcgta gtgataacgt tctttcaggt
    27661 tatcgcgcaa ttgcaaaata tgcccgatgg cgtcgtgcat tagttcgata gcgttaactc
    27721 cgcgtgctgg atcgctggag tgccccgact ggccctgaat acggatggcg ttagagatat
    27781 gacctttatg tgcgcgtacc ggttgtagtg acgtcggttc gccaatgatg gcgcaatccg
    27841 ggcgcagggc ggtagtttcg gcaaaataac gcgctccggc catactggtt tcttcatcag
    27901 cagtcgccag aatgtagagc ggttttttca gtttcgtgac gtcgacatcg cgtagcgtat
    27961 caaggataaa cgcaaaaaag cctttcatgt cggcggtgcc taagccgtaa agcttgccgt
    28021 catgctccgt cagtgtaaac ggatcgcgcg tccagcgacc gtcatcaaat ggcaccgtat
    28081 cggtatgccc cgccagcaac aagccgccag ccccctgtcc gatacttgcc agcatattga
    28141 atttgttgcg agttcctggc acaggctgca cttccacatt gaagcctaaa tctttaaacc
    28201 agtccgccag cagagtgatt aaatctgcat tgctttgatc gagtgcctct tccgtggcgc
    28261 ttattgaagg tgtggcaatc agagcacggt aaatctcgat aaatggcggt aatttgtttt
    28321 tcattgttga cacacctctg gtcatgatag tatcaatatt catgcagtat ttatgaataa
    28381 aaatacacta acgttgagcg taataaaacc caccagccgt aaggtgaatg ttttacgttt
    28441 aacctggcaa ccagacataa gaaggtgaat agccccgatg ttgaatacgc tgattgtggg
    28501 tgccagcggc tacgctggcg cagagctagt gacctatgta aatcgccatc cgcatatgaa
    28561 cataaccgct ttgactgttt cagcgcaaag caatgatgcg ggaaagttaa tctccgattt
    28621 gcatccgcag ctaaaaggca tcgttgagct gccgttgcag ccgatgtcgg atatcagcga
    28681 gtttagccca ggggtggacg tagtgtttct cgccaccgcc catgaagtta gccacgattt
    28741 agcgccgcag tttcttgaag cgggctgcgt ggtgttcgac ctttccggtg cgtttcgtgt
    28801 taacgacgcc accttctatg aaaaatatta cggctttacc catcaatacc cggaactgct
    28861 ggaacaggca gcctacggtc tggcggagtg gtgcggtaat aaattaaaag aagcgaattt
    28921 gattgcggtg ccaggctgtt atccgacggc ggcacagctg gcgctgaaac cgttgattga
    28981 tgccgatctt cttgacctca atcagtggcc ggtgatcaac gccaccagcg gcgtgagcgg
    29041 tgcaggacgt aaggcagcga tttcaaatag tttttgtgaa gttagcctgc aaccgtatgg
    29101 cgtctttact catcgccatc aaccagagat cgccacacac ctcggtgctg acgttatctt
    29161 caccccacat ctgggcaatt tcccgcgcgg cattctcgaa accattacct gccgcctgaa
    29221 atcgggtgtg acccaggcgc aagtcgcgca agtgttacag caggcgtatg cccataaacc
    29281 gctggtgcgg ctgtatgaca aaggcgttcc ggcgctgaaa aatgtcgttg ggctgccatt
    29341 ttgcgatatc gggtttgccg ttcagggcga gcatctgatt attgtggcga ccgaagacaa
    29401 cttactgaaa ggcgcggcgg cacaagcggt acagtgcgcc aatattcgtt tcggctatgc
    29461 ggaaacgcag tctcttattt aagggtgcaa tgatgaatcc attaattatc aaactgggcg
    29521 gcgtactgct ggatagtgaa gaggcgctgg aacgtctgtt tagcacactg gtgaattatc
    29581 gtgagtcaca tcagcgtccg ctggtgattg tgcacggcgg cggttgcgtg gtggatgagc
    29641 tgatgaaagg gctgaatctg ccggtgaaaa agaaaaacgg cctgcgggtg acgcctgctg
    29701 atcagataga cattatcacc ggagcactgg cgggaacggc aaataaaacc ctgttggcat
    29761 gggcgaagaa acatcagatt gcggccgtag gtttgtttct cggtgacggc gacagcgtca
    29821 aagtgaccca gcttgatgaa gagttaggtc atgttggact ggcgcagcca ggttcgccta
    29881 agcttatcaa ctccttgctg gagaacggtt atctgccggt ggtcagctcc attggcgtaa
    29941 cagacgaagg gcaactgatg aacgtcaatg ccgaccaggc ggcaacggcg ctggcggcaa
    30001 cgctgggcgc ggatctgatt ttgctctccg acgtcagcgg cattctcgac ggcaaagggc
    30061 aacgcattgc cgaaatgacc gccgcgaaag cagaacaact gattgagcag ggcattatta
    30121 ctgacggcat gatagtgaaa gtgaacgcgg cgctggatgc ggcccgcacg ctgggccgtc
    30181 cggtagatat cgcctcctgg cgtcatgcgg agcagcttcc ggcactgttt aacggtatgc
    30241 cgatgggtac gcggatttta gcttaagttt tgttggccgg aggcgcagct ttccggcatt
    30301 gaatttcaaa ataaggaaac agagttatgg cactttgggg cgggcgtttt acccaggcag
    30361 cagatcaacg gttcaaacaa ttcaacgact cactgcgctt tgattaccgt ctggcggagc
    30421 aggatattgt tggctctgtg gcctggtcca aagccctggt cacggtaggc gtgttaaccg
    30481 cagaagagca ggcgcaactg gaagaggcgc tgaacgtgct gctggaagat gttcgcgcca
    30541 ggccacaaca aatccttgaa agcgacgccg aagatatcca tagctgggtg gaaggcaaac
    30601 tgatcgacaa agtgggccag ttaggcaaaa agctgcatac cgggcgtagt cgtaacgatc
    30661 aggtagcgac tgacctgaaa ctgtggtgca aagataccgt tagcgagtta ctgacggcta
    30721 accgacagct gcaatcggcg ctggtggaaa ccgcacaaaa caatcaggac gcggtcatgc
    30781 caggttacac tcacctgcaa cgcgcccagc cggtgacgtt cgcgcactgg tgcctggcct
    30841 atgttgagat gctggcgcgt gatgaaagcc gtttgcagga tgcgcttaag cgtctggatg
    30901 tcagcccatt aggctgtggc gcgctggcgg gaacggccta tgaaatcgac cgtgaacagt
    30961 tagcaggctg gctgggcttt gcttcggcga cccgtaacag tctggacagc gtttctgacc
    31021 gtgaccatgt gttggaactg ctgtcggctg ccgctatcgg catggtgcat ctgtcgcgtt
    31081 ttgctgaaga tctgattttc tttaacaccg gcgaagcggg gtttgtggag ctttctgacc
    31141 gcgtgacttc cggttcatca ttaatgccgc agaagaaaaa cccggatgcg ctggagctga
    31201 ttcgcggtaa atgcggtcgg gtgcagggtg cgttaaccgg catgatgatg acgctgaaag
    31261 gtttgccgct ggcttacaac aaagatatgc aggaagacaa agaaggtctg ttcgacgcgc
    31321 tcgatacctg gctggactgc ctgcatatgg cggcgctggt gctggacggc attcaggtga
    31381 aacgtccacg ttgccaggaa gcggctcagc agggttacgc caacgccacc gaactggcgg
    31441 attacctggt ggcgaaaggc gtacctttcc gcgaggcgca ccatatcgtg ggtgaagcgg
    31501 tggtggaagc cattcgtcag ggcaaaccgc tggaagatct gccgctcgac gagttgcaga
    31561 aattcagccc ggtgattggc gaagatgtct atccgatttt gtcgctgcaa tcgtgcctcg
    31621 acaagcgtgc ggcaaaaggc ggcgtctcac cgcagcaggt ggcccaagcg attgcttttg
    31681 cgcaggcgcg gttggggtaa gaacatttat atgtacaaat ttgagcctgg cttatcgccg
    31741 ggctttttta tggcaaaaaa aagcggatcc tggagatccg caaaagttca cgttggcttt
    31801 agttattcga gttgagaaac tctcgaaacg ggcagtgact tcaagggtta aaagaggtgc
    31861 cgctccgttt ctgtgagcaa ttatcagtca gaatgcttga tagggataat cgttcattgc
    31921 tattctacct atcgccatga actatcgtgg cgatggagga tggataatga atattcgtga
    31981 tcttgagtac ctggtggcat tggctgaaca ccgccatttt cggcgtgcgg cagattcctg
    32041 ccacgttagc cagccgacgc ttagcgggca aattcgtaag ctggaagatg agctgggcgt
    32101 gatgttgctg gagcggacca gccgtaaagt gttgttcacc caggcgggaa tgctgctggt
    32161 ggatcaggcg cgtaccgtgc tgcgtgaggt gaaagtcctt aaagagatgg caagccagca
    32221 gggcgagacg atgtccggac cgctgcatat tggtttgatt cccacggttg gaccgtacct
    32281 gctaccgcat attatcccga tgctgcacca gacctttcca aagctggaaa tgtatctgca
    32341 tgaagcacag acccaccagt tactggcgca actggacagt ggcaaactcg attgcgtgat
    32401 cctcgcgctg gtgaaagaga gcgaagcatt cattgaagtg ccgttgtttg atgagccaat
    32461 gttgctggct atctatgaag atcacccgtg ggcgaaccgc gaatgcgtac cgatggccga
    32521 tctggcaggg gaaaaactgc tgatgctgga agatggtcac tgtttgcgcg atcaggcaat
    32581 gggtttctgt tttgaagccg gggcggatga agatacacac ttccgcgcga ccagcctgga
    32641 gacgctgcgc aacatggtgg cggcaggtag cgggatcact ttactgccag cgctggctgt
    32701 gccgccggag cgcaaacgcg atggggttgt ttatctgccg tgcattaagc cggaaccacg
    32761 ccgcactatt ggcctggttt atcgtcctgg ctcaccgctg cgcagccgct atgagcagct
    32821 ggcagaggcc atccgagcaa gaatggatgg ctatttcgat aaagtgttaa aacaggcggt
    32881 ttaaaccgtt taacgcagct acccgatagg cttccgccat ggtcgggtag ttaaaggtgg
    32941 tgttgacgaa gtactcaata gtgttgccgc cacctttctg ttccataatc gcctgaccga
    33001 tatgaataat ttcggcagcg cgctcgccaa agcagtgaat acccagaatc tcttttgttt
    33061 cccgatggaa caaaattttc agcgtgccca cgttcatgcc gacgatttgt gcgcgtgcca
    33121 gatgtttaaa ctgggcgcgg cccacttcat atggcacttt catcgcggtc agctgctgtt
    33181 cggttttgcc cacagagctg atttccggga tggtgtaaat gccggtaggg atatcttcaa
    33241 tcagatgcgc ggtggcttcg ccttttacca gcgcctgcgc ggcaatgcgc ccctggtcat
    33301 aggccgctga tgccaggctc ggataaccaa tcacgtcgcc caccgcgtaa acgtgcggct
    33361 gtgcggtctg atacatgctg ttgaccttca gctgtccgcg actgtcagtt tccagcccaa
    33421 tgttctgtaa cgccagcgaa tcggtattac cggtacgacc gttggcatag agcaggcagt
    33481 cagctttcag ttttttaccc gacttcagat gcatgatcac accgtcgtca cagccttcga
    33541 tcttctcgta ctcttcgttg tgacgaatca ctacgccact gttccagaag tgataggaga
    33601 gagaatctga catctcttga tcgagaaatg ccagcaggcg atcgcgggtg ttgatcagat
    33661 ccacttttac atccataccg cggaagatcg acgcatattc acagccgatc actccagcac
    33721 catagataag tacatggcgc ggttcgtggt gcatgctaag aattgagtcg ctgtcgtaaa
    33781 tgcgtggatg ggtgaaatca acatctgttg gatgatatgg acgagagccg caggcaataa
    33841 caaatttttc agcggttagt gtttcgacgc tgccgtccgg gcaatccagc gccaacgtat
    33901 gctcgtcaac aaagcgagcg ttcccctgca atatttcaca gtgattacgt tcgtaaaatc
    33961 cctgacgcat gcgcgtttgt tgattaatca cgttatcggc atggttaagg atatcggcaa
    34021 aagaagagcg gagcagtcgc gaatggtcgc tgtatagtgg gttttgattg aattctataa
    34081 tgcggctgac ggcgtgacgg agagctttcg acgggatggt gccccagtgg gtgcaaccgc
    34141 cgccaacatt ttgataacgc tcgataactg cgacgcgcac accttgctta accaggccca
    34201 ttgcagcgcc ttcgccgccg gggccggaac ctattactat ggcatcgtaa tcgtaggaat
    34261 gtggcatggt agggcttacc tgttcttata cataaaagca acagaatggt aacattctat
    34321 cgcgggtaag ccaattgatc cccgtcattt atctggctat atcctgagcg gcctttgctt
    34381 tgtctgtttc ttacttttgc cctgacgttt tattggattt ttatcgacga tactctccgt
    34441 ttaagcggca ggtttccgct gtacgtaaaa gaaccggcca gtaaatatgt tttattgcgt
    34501 taccgttcat tcacaatact ggagcaatcc agtatgttca ttctctggta tagtgccagc
    34561 agtacttttg gcaaggattc agacatcgtg atgggcataa gagcgcaaca aaaagaaaaa
    34621 acccgccgtt cgctggtgga agccgcattt agccaattaa gtgctgaacg cagcttcgcc
    34681 agcctgagtt tgcgtgaagt ggcgcgtgaa gcgggcattg ctcccacctc tttttatcgg
    34741 catttccgcg acgtagacga actgggtctg accatggttg atgagagcgg tttaatgcta
    34801 cgccaactca tgcgccaggc gcgtcagcgt atcgccaaag gcgggagtgt gatccgcacc
    34861 tcggtctcca catttatgga gttcatcggt aataatccta acgccttccg gttattattg
    34921 cgggaacgct ccggcacctc cgctgcgttt cgtgccgccg ttgcgcgtga aattcagcac
    34981 ttcattgcgg aacttgcgga ctatctggaa ctcgaaaacc atatgccgcg tgcgtttact
    35041 gaagcgcaag ccgaagcaat ggtgacaatt gtcttcagtg cgggtgccga ggcgttggac
    35101 gtcggcgtcg aacaacgtcg gcaattagaa gagcgactgg tactgcaact gcgaatgatt
    35161 tcgaaagggg cttattactg gtatcgccgt gaacaagaga aaaccgcaat tattccggga
    35221 aatgtgaagg acgagtaatg aaacaagcaa atcaagatag aggtacgctg ctgctggcgt
    35281 tagttgctgg cttatcgatt aatggtactt tcgcagcgct gtttagctcc attgtgccat
    35341 tttctgtatt cccgattatt tccctggtgc tgacggttta ctgcctgcat caacgttatc
    35401 ttaatcgcac tatgccggta ggcttgccgg gtctggcagc tgcctgtttt attctcggcg
    35461 tactgctgta cagcacggta gttcgtgcgg aatatccgga tatcggctct aacttcttcc
    35521 cggcagtact ctccgtcatt atggtgttct ggattggcgc gaagatgcgt aaccgtaagc
    35581 aggaagttgc tgagtaatcg gtattatgcc ggatgctggc gcatccggca tgggttttac
    35641 ttcgcggtca gtaatacgcc gcactccata tggtgcgtgt aggggaactg atcaaacaga
    35701 gccagacgtt cgaccttgtg cgtctggctt aatgtttcca gattcttgca taacgtttcc
    35761 gggttacagg agatgtacaa aatacacgga tacgcctgca ccattttctc ggtttcactg
    35821 tccagaccgc tgcgcggtgg gtcgacaaaa atggtttcgc actgataact ctttaagtcg
    35881 atcccttgca ggcggttaaa ctcgcgcaca ccattcatcg cctgagtaaa ttcttctgcc
    35941 gccatacgaa taatttgtac gttatcaata tggttagctg cgatgttgta ttgcgcagca
    36001 gcaaccgacg gcttagcgat ttcggtggct aatacccgat caaaattgcg cgccagcgct
    36061 aatgaaaagt taccgttgcc gcagtacagc tccagtaaat cgccttttga gcctttggtt
    36121 acgtccagcg cccattccag catctgaata ttcatcgccg cgttcggctg ggtaaagctg
    36181 ttttctacct gacggtagat catctctttc cctgcgaccg gcagacgctc gtcgatgtaa
    36241 tcttgatcca gctcgatttt ggttttcgtt gcccgaccaa tcagatgcac attcagattc
    36301 tgcgcgcgca gtgcatcgcg cagggcctcc gcttcctgac gccactcatc atccagcttc
    36361 ttatggtata gcagggaaac caccgcctga ttactcagcg tggtgaggta atcaatctgg
    36421 aacaacttgt ggcgcagaac gggattatta cgcacacccg caatcatcgc cgtcatcaac
    36481 tggttgataa gttcactggc ggcggggaag ctatccacgc ggatacggct tttggtttgt
    36541 tgatcgaaaa tgatgtgata caggtcatcg ccatcgtgcc agatgcggaa ctccgcacgc
    36601 atccggtaat gactgaccgg cgagcgaaac acttccggaa ccaggtcaga aaacggtgcc
    36661 atcatacttt gcaaacgtac cactttttcg gctaactgcg cttcatactg ttctgttgga
    36721 aggtgttcgg gggtcatgat gtatcctgaa aaattaaagt acgcggcgat tgtagggatt
    36781 gctcatcaga tgtccagatc ttgatgaatt cctatttgtg agctacgtct ggacagtaac
    36841 ttgttacaac ctgtagcatc cacttgtcgg tcctgtgagt taatagggaa tccagtgcga
    36901 atctggagct gacgcgcagc ggtaaggaaa ggtgcgatga ttgcgttatg cggacactgc
    36961 catccggtgg gaagtcatca tctcttagta tcttagatac ccctccaagc ccgaagacct
    37021 gccggccaac gtcgcatctg gttctcatca tcgcgtaata ttgatgaaac ctgcggcatc
    37081 cttcttctat tgtggatgct ttacaatgat taaaaaagct tcgctgctga cggcgtgttc
    37141 tgtcacagcc ttttccgctt gggcacagga taccagcccg gatactctcg tcgttactgc
    37201 taaccgtttt gaacagccgc gcagcactgt gcttgcacca accaccgttg tgacccgtca
    37261 ggatatcgac cgctggcagt cgacctcggt caatgatgtg ctgcgccgtc ttccgggcgt
    37321 cgatatcacc caaaacggcg gttcaggtca gctctcatct atttttattc gcggtacaaa
    37381 tgccagtcat gtgttggtgt taattgatgg cgtacgcctg aatctggcgg gggtgagtgg
    37441 ttctgccgac cttagccagt tccctattgc gcttgtccag cgtgttgaat atatccgtgg
    37501 gccgcgctcc gctgtttatg gttccgatgc aataggcggg gtggtgaata tcatcacgac
    37561 gcgcgatgaa cccggaacgg aaatttcagc agggtgggga agcaatagtt atcagaacta
    37621 tgatgtctct acgcagcaac aactggggga taagacacgg gtaacgctgt tgggcgatta
    37681 tgcccatact catggttatg atgttgttgc ctatggtaat acaggaacgc aagcgcagac
    37741 agataacgat ggttttttaa gtaaaacgct ttatggcgcg ctggagcata actttactga
    37801 tgcctggagc ggctttgtgc gcggctatgg ctatgataac cgtaccaatt atgacgcgta
    37861 ttattctccc ggttcaccgt tgctcgatac ccgtaaactc tatagccaaa gttgggacgc
    37921 cgggctgcgc tataacggcg aactgattaa atcacaactc attaccagct atagccatag
    37981 caaagattac aactacgatc cccattatgg tcgttatgat tcgtcggcga cgctcgatga
    38041 gatgaagcaa tacaccgtcc agtgggcaaa caatgtcatc gttggtcacg gtagtattgg
    38101 tgcgggtgtc gactggcaga aacagactac gacgccgggt acaggttatg ttgaggatgg
    38161 atatgatcaa cgtaataccg gcatctatct gaccgggctg caacaagtcg gcgattttac
    38221 ctttgaaggc gcagcacgca gtgacgataa ctcacagttt ggtcgtcatg gaacctggca
    38281 aaccagcgcc ggttgggaat tcatcgaagg ttatcgcttc attgcttcct acgggacatc
    38341 ttataaggca ccaaatctgg ggcaactgta tggcttctac ggaaatccga atctggaccc
    38401 ggagaaaagc aaacagtggg aaggcgcgtt tgaaggctta accgctgggg tgaactggcg
    38461 tatttccgga tatcgtaacg atgtcagtga cttgatcgat tatgatgatc acaccctgaa
    38521 atattacaac gaagggaaag cgcggattaa gggcgtcgag gcgaccgcca attttgatac
    38581 cggaccactg acgcatactg tgagttatga ttatgtcgat gcgcgcaatg cgattaccga
    38641 cacgccgttg ttacgccgtg ctaaacagca ggtgaaatac cagctcgact ggcagttgta
    38701 tgacttcgac tggggtatta cttatcagta tttaggcact cgctatgata aggattactc
    38761 atcttatcct tatcaaaccg ttaaaatggg cggtgtgagc ttgtgggatc ttgcggttgc
    38821 gtatccggtc acctctcacc tgacagttcg tggtaaaata gccaacctgt tcgacaaaga
    38881 ttatgagaca gtctatggct accaaactgc aggacgggaa tacaccttgt ctggcagcta
    38941 caccttctga accacgtccc accgtgctgg tgtttgactc cggcgtcggt gggttgtcgg
    39001 tctatgacga gatccggcat ctcttaccgg atctccatta catttatgct ttcgataacg
    39061 tcgctttccc gtatggcgaa aaaagcgaag cgtttattgt tgagcgagtg gtggcaattg
    39121 tcaccgcggt gcaagaacgt tatccccttg cgctggctgt ggtcgcttgc aacactgcca
    39181 gtaccgtttc acttcctgca ttacgcgaaa agttcgactt cccggttgtt ggtgtcgtgc
    39241 cggcgattaa acctgctgca cgtctgacgg caaatggcat tgtcggatta ctggcaaccc
    39301 gcggaacagt taaacgttct tatactcatg agctgatcgc gcgtttcgct aatgaatgcc
    39361 agatagaaat gctgggctcg gcagagatgg ttgagttggc tgaagcgaag ctacatggcg
    39421 aagatgtttc tctggatgca ctaaaacgta tcctacgccc gtggttaaga atgaaagagc
    39481 cgccagatac cgttgtattg ggttgcaccc atttccctct actacaagaa gaactgttac
    39541 aagtgctgcc agagggaacc cggctggtgg attctggcgc agcgattgct cgccgaacgg
    39601 cctggttgtt agaacatgaa gccccggatg caaaatctgc cgatgcgaat attgcctttt
    39661 gtatggcaat gacgccagaa gctgaacaat tattgcccgt tttacagcgt tacggcttcg
    39721 aaacgctcga aaaactggca gttttaggct gatttggttg aatgttgcgc ggtcagaaaa
    39781 ttattttaaa tttcctcttg tcaggccgga ataactccct ataatgcgcc accactgaca
    39841 cggaacaacg gcaaacacgc cgccgggtca gcggggttct cctgagaatc tcaacagaga
    39901 aaagcaaaaa aaatgcttga ctctgtagcg ggaaagcgta ttatgcacac c
//
LOCUS       DAZPDU010000017        39460 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZPDU010000017 DAZPDU010000000
VERSION     DAZPDU010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39460
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.017"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..1166)
                     /gene="dnaK"
                     /locus_tag="ACHURS_000817"
     CDS             complement(<1..1166)
                     /gene="dnaK"
                     /locus_tag="ACHURS_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFR1236622.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDV"
     gene            1543..1947
                     /locus_tag="ACHURS_000818"
     CDS             1543..1947
                     /locus_tag="ACHURS_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFR1236623.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            1973..2686
                     /gene="msyB"
                     /locus_tag="ACHURS_000819"
     CDS             1973..2686
                     /gene="msyB"
                     /locus_tag="ACHURS_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFR1236624.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVIQIACLRRMVSATQV"
     gene            2835..3401
                     /gene="satP"
                     /locus_tag="ACHURS_000820"
     CDS             2835..3401
                     /gene="satP"
                     /locus_tag="ACHURS_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFR1236625.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(3436..4023)
                     /gene="mog"
                     /locus_tag="ACHURS_000821"
     CDS             complement(3436..4023)
                     /gene="mog"
                     /locus_tag="ACHURS_000821"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFR1236626.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(4138..5091)
                     /gene="tal"
                     /locus_tag="ACHURS_000822"
     CDS             complement(4138..5091)
                     /gene="tal"
                     /locus_tag="ACHURS_000822"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFR1236627.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            5370..6799
                     /locus_tag="ACHURS_000823"
                     /pseudo
     CDS             5370..6799
                     /locus_tag="ACHURS_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
     gene            6869..7645
                     /gene="yaaA"
                     /locus_tag="ACHURS_000824"
     CDS             6869..7645
                     /gene="yaaA"
                     /locus_tag="ACHURS_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFR1236628.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQHLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(7798..8094)
                     /locus_tag="ACHURS_000825"
     CDS             complement(7798..8094)
                     /locus_tag="ACHURS_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFR1236629.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(8308..9594)
                     /gene="thrC"
                     /locus_tag="ACHURS_000826"
     CDS             complement(8308..9594)
                     /gene="thrC"
                     /locus_tag="ACHURS_000826"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFR1236630.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALCDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(9595..10527)
                     /gene="thrB"
                     /locus_tag="ACHURS_000827"
     CDS             complement(9595..10527)
                     /gene="thrB"
                     /locus_tag="ACHURS_000827"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFR1236631.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCLELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(10529..12991)
                     /gene="thrA"
                     /locus_tag="ACHURS_000828"
     CDS             complement(10529..12991)
                     /gene="thrA"
                     /locus_tag="ACHURS_000828"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFR1236632.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEGELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     MERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(13018..13137)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(13073..13138)
                     /gene="thrL"
                     /locus_tag="ACHURS_000829"
     CDS             complement(13073..13138)
                     /gene="thrL"
                     /locus_tag="ACHURS_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFR1236633.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(13352..14038)
                     /locus_tag="ACHURS_000830"
     CDS             complement(13352..14038)
                     /locus_tag="ACHURS_000830"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFR1236634.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDVIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(14438..14578)
                     /gene="yjjY"
                     /locus_tag="ACHURS_000831"
     CDS             complement(14438..14578)
                     /gene="yjjY"
                     /locus_tag="ACHURS_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFR1236635.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            14674..15390
                     /gene="arcA"
                     /locus_tag="ACHURS_000832"
     CDS             14674..15390
                     /gene="arcA"
                     /locus_tag="ACHURS_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFR1236636.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(15450..16802)
                     /gene="creD"
                     /locus_tag="ACHURS_000833"
     CDS             complement(15450..16802)
                     /gene="creD"
                     /locus_tag="ACHURS_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFR1236637.1"
                     /translation="MLKSSLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFL
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSVDSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(16860..18284)
                     /gene="creC"
                     /locus_tag="ACHURS_000834"
     CDS             complement(16860..18284)
                     /gene="creC"
                     /locus_tag="ACHURS_000834"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFR1236638.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLDQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(18284..18973)
                     /gene="creB"
                     /locus_tag="ACHURS_000835"
     CDS             complement(18284..18973)
                     /gene="creB"
                     /locus_tag="ACHURS_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFR1236639.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(18986..19459)
                     /gene="creA"
                     /locus_tag="ACHURS_000836"
     CDS             complement(18986..19459)
                     /gene="creA"
                     /locus_tag="ACHURS_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFR1236640.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            19670..20539
                     /gene="robA"
                     /locus_tag="ACHURS_000837"
     CDS             19670..20539
                     /gene="robA"
                     /locus_tag="ACHURS_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFR1236641.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(20536..21183)
                     /gene="gpmB"
                     /locus_tag="ACHURS_000838"
     CDS             complement(20536..21183)
                     /gene="gpmB"
                     /locus_tag="ACHURS_000838"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFR1236642.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            21235..21756
                     /gene="yjjX"
                     /locus_tag="ACHURS_000839"
     CDS             21235..21756
                     /gene="yjjX"
                     /locus_tag="ACHURS_000839"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFR1236643.1"
                     /translation="MHQVICATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(21841..22167)
                     /gene="trpR"
                     /locus_tag="ACHURS_000840"
     CDS             complement(21841..22167)
                     /gene="trpR"
                     /locus_tag="ACHURS_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFR1236644.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(22257..24194)
                     /gene="sltY"
                     /locus_tag="ACHURS_000841"
     CDS             complement(22257..24194)
                     /gene="sltY"
                     /locus_tag="ACHURS_000841"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFR1236645.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQVQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDVIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQVDLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            24405..26072
                     /gene="ettA"
                     /locus_tag="ACHURS_000842"
     CDS             24405..26072
                     /gene="ettA"
                     /locus_tag="ACHURS_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFR1236646.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(26233..26340)
                     /gene="nadS"
                     /locus_tag="ACHURS_000843"
                     /pseudo
     CDS             complement(26233..26340)
                     /gene="nadS"
                     /locus_tag="ACHURS_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
     gene            complement(26379..27611)
                     /gene="nadR"
                     /locus_tag="ACHURS_000844"
     CDS             complement(26379..27611)
                     /gene="nadR"
                     /locus_tag="ACHURS_000844"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFR1236647.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(27632..29014)
                     /gene="radA"
                     /locus_tag="ACHURS_000845"
     CDS             complement(27632..29014)
                     /gene="radA"
                     /locus_tag="ACHURS_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFR1236648.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(29063..30031)
                     /gene="serB"
                     /locus_tag="ACHURS_000846"
     CDS             complement(29063..30031)
                     /gene="serB"
                     /locus_tag="ACHURS_000846"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFR1236649.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            30137..30781
                     /locus_tag="ACHURS_000847"
     CDS             30137..30781
                     /locus_tag="ACHURS_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFR1236650.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            30809..31825
                     /gene="lplA"
                     /locus_tag="ACHURS_000848"
     CDS             30809..31825
                     /gene="lplA"
                     /locus_tag="ACHURS_000848"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFR1236651.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            31857..32120
                     /locus_tag="ACHURS_000849"
                     /pseudo
     CDS             31857..32120
                     /locus_tag="ACHURS_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(32281..33000)
                     /gene="deoD"
                     /locus_tag="ACHURS_000850"
     CDS             complement(32281..33000)
                     /gene="deoD"
                     /locus_tag="ACHURS_000850"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFR1236652.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(33080..34303)
                     /gene="deoB"
                     /locus_tag="ACHURS_000851"
     CDS             complement(33080..34303)
                     /gene="deoB"
                     /locus_tag="ACHURS_000851"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFR1236653.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAVGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(34355..35677)
                     /gene="deoA"
                     /locus_tag="ACHURS_000852"
     CDS             complement(34355..35677)
                     /gene="deoA"
                     /locus_tag="ACHURS_000852"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFR1236654.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(35804..36583)
                     /gene="deoC"
                     /locus_tag="ACHURS_000853"
     CDS             complement(35804..36583)
                     /gene="deoC"
                     /locus_tag="ACHURS_000853"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFR1236655.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            36841..38391
                     /locus_tag="ACHURS_000854"
     CDS             36841..38391
                     /locus_tag="ACHURS_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFR1236656.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCKKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            38363..39226
                     /locus_tag="ACHURS_000855"
     CDS             38363..39226
                     /locus_tag="ACHURS_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFR1236657.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQHLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
BASE COUNT         9339 a        10702 c         9927 g         9492 t
ORIGIN      
        1 acgtctttta cgtcaccagt cagaacacca ccctgaacag cagcaccgat tgctacagct
       61 tcgtccgggt taacgtcttt acgcggctct ttaccaaaga actcagcaac tttcttctga
      121 accattggca tacgagtctg accacccacg aggataacgt cgtcgatatc agatacggac
      181 aggccagcgt cctgcagtgc aactttcagc ggctcaatgg aacggtttac cagatcttca
      241 accaggcttt ccagtttcgc acgagtcact ttgatgttca tgtgtttcgg accggtcgca
      301 tctgcagtga tgtacggcag gttaacgtcg gtctgctgag cggaagacag ttcgattttc
      361 gctttttctg ccgcttcttt caggcgctgc attgccagcg gatcgttgcg caggtcaatg
      421 ccctgatctt tcttgaattc ttcaaccagg tagttgatca gacggctgtc gaagtcttca
      481 ccccccaggt gggtatcacc gttggtagcc agaacttcga aggttttttc gccgtcaact
      541 tcgtcgattt cgataataga aatatcgaaa gtaccaccac ccaggtcata aaccgcgata
      601 gtacggttgc cagtaccttt gtccagaccg taagccagcg cagctgcggt cggttcgttg
      661 atgatacgtt ttacttccag accagcgata cggcctgcgt ctttggttgc ctgacgctga
      721 gcatcgttaa agtatgccgg tacggtgata acagcttcag ttaccggttc acccaagtaa
      781 tcttcagcgg ttttcttcat ttttttcagc acttcagcag aaatctgcgg cggtgccatt
      841 ttctggcctt taacttcgac ccatgcgtcg ccgttatcag cagcaataat tttgaacggc
      901 atgatggaaa catcacgctg tacttcttcg tcctggaagc ggcggccaat caggcgttta
      961 atcgcaaaca gggtgttttg cgggttcgtc actgcctgac gtttagccgg ctgaccaacc
     1021 agagtttcac catcctgggt ataggcaata atagaaggcg tggtgcgatc gccttcggcg
     1081 ttctccagca cgcgaggagt ggtgccatcc ataatcgcta cacaagagtt ggtagtaccc
     1141 aggtcgatac caattatttt acccatctaa acgtctccac tatatattcg gtcatcatgt
     1201 ggttgtgagt ctgtaatagg ggcgaaacgt ctggtttcaa ctgcccaatt tcattttttt
     1261 gcagactcac tcactgcggt tgactactaa atggggtcgt aacccacgtc atcaaggggg
     1321 agatgcaaaa attttttgtg cttttgcgca gaaatttcgc gatatggcag gacaagacgt
     1381 aaaattgaca gcaattttat tcatataaag aatgaatcgc agccaaggca ttcatcaaaa
     1441 aattgtaata aaaagaaaag attacgtacc tgaatcttct ctttatcagc agtaaactag
     1501 tgggtattca tccctctacc tcttcccact aagagaatcc ttatgaaatc cgtttttacg
     1561 atttccgcca gcctggcgat tagcctgatg ctgtgctgca cggcgcaggc aaacgaccat
     1621 aaactcctcg gcgtcattgc aatgccgcgt aacgaaacca acgatctggc gctgaaactt
     1681 cctgtttgtc gcattgtgaa acgcatacaa ctctctgccg accatggcga tttacagtta
     1741 agcggtgcat cggtttattt caaagccgcc cgtagcgcca gtcagagtct gaatattcct
     1801 tcagaaataa aagaagggca aaccactgac tggatcaaca ttaacagtga taacgacaat
     1861 aaacgctgcg tctcaaaaat caccttttcc ggtcatacgg tgaactcatc ggatatggcc
     1921 acgctgaaaa ttatcggcga cgactaacgc cagaatatag aaaccacaaa aaatgaatgt
     1981 taattacctg aatgattcag atctggattt tcttcagcat tgtagtgagg aacagttggc
     2041 aaatttcgcc cgattgctca cccataatga aaaaggcaaa actcgcctct ccagcgtact
     2101 gatgcgcaac gaactgttta aatcgatgga agggcatccc gagcaacatc gccgcaactg
     2161 gcagctgatt gctggagaat tacagcattt tggtggcgat agtatcgcca acaaactgcg
     2221 cggacacggt aaattgtatc gggccatttt gctcgatgtt tcaaaacgat tgaagctgaa
     2281 agccgacaaa gagatgtcta cgtttgagat tgagcaacag ttactggaac aatttctgcg
     2341 taacacctgg aagaatatgg acgaggaaca taagcaggag tttctgcacg cggtcgatgc
     2401 cagggtgaat gagctggaag agctgctgcc gctgctgatg aaagacaaat tattggcaaa
     2461 aggcgtgtcg catttgcttt ccagccaatt gacccgcatt ttacgcaccc acgcagcaat
     2521 gagcgtgctt gggcatggtt tgctgcgcgg cgcgggtctg ggaggccctg tgggcgcagc
     2581 attaaatggg gttaaagcgg tcagcggcag tgcctatcgc gtgacaattc cagccgtaat
     2641 acaaatcgcc tgcctgcgcc ggatggttag cgccactcag gtctgacacc gtaaaccggg
     2701 gaaaattttc attttcccct caaaagatcg tagacactgc cccactggct gattattatg
     2761 ccgcgccctg aaaacactac tgttattcag ggaaattatt tcaccattca ttcgatgatg
     2821 atttttgagg aattatgggc aacactaagt tggctaatcc ggcaccgctg ggcctgatgg
     2881 gcttcggcat gaccaccatt ctgcttaacc tgcacaacgt gggttatttc gctctggacg
     2941 gtattattct tgccatgggc attttctacg gcggcatcgc gcaaattttt gccggtctgc
     3001 tggagtacaa aaaaggcaac actttcggtt taaccgcatt cacctcttac ggttctttct
     3061 ggttgacgct ggttgcgatt ctgctgatgc cgaaactggg tctgaccgat gcgccaaatg
     3121 cacagttcct tggtgtctac ctgggtctgt ggggcgtatt tacgctgttt atgttcttcg
     3181 gcacgctgaa aggcgcacgc gttctgcaat tcgttttctt tagcctgact gtgctgtttg
     3241 ccctgctggc gatcggtaac attgccggta acgccgcaat catccacttt gccggctgga
     3301 ttggtctgat ctgtggtgcc agtgcaatct atctggcgat gggtgaagta ctgaacgagc
     3361 agtttggtcg caccgttctg ccgattggtg aatcccacta attgttttga gatccccccg
     3421 ctcgggggga tttttttatt cgctaacgtc gcgtcttgca ctcttcggtc tgaatgcagc
     3481 aaccacttct ggtgccgttt caacgtatgg cccttccagc aactgaatgc agtacggtac
     3541 gctggcaaaa ataccgtgta ccacaacgtt acccgcagcg tccttcacac cttccagcgt
     3601 ctctttaata gacttcggct gaccgggtaa gttaaggatc agcgcctgtt tgcgaatcac
     3661 accaacctga cgcgaaagga tcgcagttgg tacaaaatgc aggcttatct ggcgcatctg
     3721 ttcaccaaag ccaggcatct cgcggtccgc tactgccagc gtcgcatcgg gcgttacgtc
     3781 acgacgcgcc gggccagttc cgcccgtggt gagcaccaga tggcaactca tttcatccac
     3841 tagctcacac aacgtttgct cgatgatcgc ctgctcatcg gggattaagc gggtttccag
     3901 ttcaaacggc gtggttagcg ccgatgtcag ccattcttcc agcgcaggga tgcctttatc
     3961 ctgataaacg ccgctggatg cgcgatcaga gatggaaact aagccaatac gtaaagtatt
     4021 catcaagatt tttccgttta aacagtctcg ttaaacagaa tgatacactg cgaagggagt
     4081 gacagacagg cttcagaaga ggtagcgtga ccgacttccc ggtcacgcta agaatgatta
     4141 cagcagatcg ccgatcattt tttccagttt ttcctggtca atagcaaact tacggatacc
     4201 ttccgccagt ttatctactg ccattggatc ctggttgtgc tgccacagga actcggactc
     4261 agtgatacgc gccggacgcg ctttcacttc gccggtgtaa gacagtttac gttcgatagc
     4321 cccttcgctc tcagccagct ctttcagcag tgccggtgcg atggtaagac ggtcgcagcc
     4381 tgccagttcc agaatttcgc cgatgttacg gaagcttgcg cccataacca cggtttcata
     4441 accgtgctct ttgtagtact ggtagatttc agatacagaa accacgcccg gatcttctgc
     4501 cggagcgtac tctttcttat cagtattcgc tttgtaccag tcaagaatac ggccaacaaa
     4561 tggcgagatc aggaacacgc ctgcttccgc acaagcacga gcctgagcga aggagaacag
     4621 cagggtcagg ttacagttga tgccttcttt ttccagctgt tctgcagcac ggataccctg
     4681 ccaggtagaa gccagtttga tcagaatacg atcgttgcta ataccagcat cgttgtagag
     4741 tttgatcagg cgttttgctt tcgcaattga cgcttcggtg tcataggaaa gacgcgcatc
     4801 aacttcagtt gagatacggc ccggaaccag tttcaggatt tccagaccaa tatttactgc
     4861 cagtttgtcg gtcgcgtcca cgatctgctg cgcgcgatcg ttgctctgct gtttcgccca
     4921 ggcgacagca tcatcaatca acttacgata ttccggaatc tgcgctgcgt taagaatgag
     4981 agaagggttg gttgtggcat cctgcggttg atacagcttc attgccgcga tgtccccagt
     5041 gtcggccact acggtggtgt actgacgaag ggaggtcaat ttgtccgtca tgatagtatt
     5101 tctctttaaa cagcttgtta gggggatgta accggtctgc cctgatgata tcacgacgca
     5161 acgtcagcgc aaccgccgac aagacggtta tcgccaggcg agactgtttc ggatttctga
     5221 cactgcgttg atatcgcccg ccatttttat acaaaacctc atgtatgcta cgcagaagtt
     5281 atcaagtacc tcgtagcgta tatacttctt aaacaattgg taacgtttac acaggaaagt
     5341 catcgcgacc ggcaataaga gggatatgca tgccagattt tttctccttt attaacagcg
     5401 tcctttgggg atcggtaatg atttacctgc tcttcggcgc aggttgttgg ttcacttttc
     5461 gcaccggatt tgtgcagttt cgctacatcc gccagtttgg caaaagtctt aaaaatagca
     5521 ttcatccaca gccaggcggt ttaacctcat ttcagtcatt gtgtaccagt cttgcggcgc
     5581 gcgtgggtag cggcaacctg gccggcgttg cgctggctat taccgccggt ggacctggag
     5641 ccgtcttttg gatgtgggtt gccgcgttta tcggcatggc gacctcgttt gccgaatgtt
     5701 cccttgcaca actttataaa gaacgtgacg tcaatggaca gtttcgtgga ggaccggcat
     5761 ggtatatggc gcgcgggctg gggatgcgtt ggatgggcgt tctgttcgcc gtctttttgc
     5821 tcatcgccta cggcataatt ttcagcggag ttcaggcgaa cgccgttgcc cgagccctga
     5881 gttttctttt gattttcccc cgctggtgac aggcattatt ctcgctgtct ttgctctgct
     5941 ggcaatcact cgcggtcttc atggcgtcgc ccggctcatg caggggtttg tcccgttgat
     6001 ggcgataatc tgggtgctga ccagcatggt gatttgcgta ataaatatcg ggcaacttcc
     6061 ccacgtcatt tggtctattt ttgagagtgc ttttggctgg caggaagcgg caggcggcgc
     6121 ggcgggatat accttaagcc aggcgattac taacggtttt cagcgcagta tgttttccaa
     6181 tgaggcggga atgggttcga cgccaaacgc ggcagcggca gcggcgtcct ggcctccgca
     6241 tccggcagcg caagggattg tccagatgat tggcattttt atcgacaccc tggtcatctg
     6301 tacggcaagc gccatgctga tattactggc gggtaacgac acaacctaca tgccgctgga
     6361 aggtattcag cttatccaga aggcgatgcg ggtgctaatg ggttcctggg gtgctgagtt
     6421 tgttaccctc gtggttattc tgtttgcctt cagctccatc gttgccaact acatttatgc
     6481 cgaaaacaat ctcttctttt tacgcctgaa caaccctaaa gcgatctggt gtttgcggat
     6541 ctgcaccttc gcaacggtca tcggcggcac cttgctaagt cttccgctga tgtggcaact
     6601 ggcagatatc ataatggcct gcatggctat taccaatttg accgccattt tactgctctc
     6661 gcctgtggtt cataccattg ccagtgatta tctacgccag cgtaaactcg gcgtgcgccc
     6721 ggtgtttgat ccgttgcgtt atccggatat cggtcgccag ctttctccgg acgcgtggga
     6781 tgatgtttcg caggagtaat cacaactatc gatcaactca ttctcttttt ttgctaaagt
     6841 cggcataaat ttcctgcaag gactggatat gctgattctt atttcacctg cgaaaacgct
     6901 tgattaccaa agcccgttga ccaccacgcg ctatacgctg ccggagctgt tagacaattc
     6961 ccagcacttg atccatgaag cgcggaaact gacgcctccg cagattagca cgctgatgcg
     7021 catcagcgac aaactggcgg gtatcaacgc cgctcgcttt catgactggc agccagattt
     7081 cacgccggag aatgcccgcc aggctatttt ggcgtttaaa ggtgatgtct acaccggctt
     7141 gcaggccgaa actttcagcg aagacgattt cgattttgcc caacagcatt tgcgaatgct
     7201 ttccggcttg tatggcgtac tgcgcccgct cgatttaatg cagccttatc gtctggaaat
     7261 ggggatccgt cttgagaatg cccgagggaa agatctgtat caattctggg gagatatcat
     7321 caccaacaag ctgaacgagg cgctcgcagc acaaggcgat aatgtggtga ttaatctggc
     7381 gtcagatgag tattttaaat cggtgaagcc gaagaaattg aatgccgaga ttatcaagcc
     7441 ggtgttcctc gatgagaaga acggcaaatt taagataatc agcttctacg ctaagaaagc
     7501 acgcggtctg atgagtcgtt tcattattga aaatcggctg accaaaccag agcaactgac
     7561 tggttttaat agcgaaggtt acttctttga tgaagcttcc tccagcaatg gcgaactggt
     7621 gtttaaacgc tacgagcagc gttaagtttg cagtcaatgc cggatgcggc gtgaacgcct
     7681 tatccggcct acaaaagcat gcagattcaa tatattgcag aattcacgta ggcctgataa
     7741 gacgcgccag cgtcgcatca ggcgctgaat gccggattgt tacccggcat ttgtcattta
     7801 gcggtgatgt ttgcctggac catgaccgcc gtgatgatca tgaggagctt tcttatggtg
     7861 gcgcggcggt ggcggcggtc cgtgtgggtg ccagcgattg cctcgccatt cataatgttg
     7921 tttccaccag ccgtggtcgc gccagtgacc tccatcccag taatagccac gattatcacg
     7981 atcgcctatc tgtaatttta ctgacgggac taacgtaatt tccgcagcct gtgctgccat
     8041 gggagcgacc agaaccaggg aaagtgcgag tacgatagat tgcatctttt tcacaggtta
     8101 ctccttttat cattgcccgt tatgggtcga tatccggaga ttacgcgatg agaacgacgg
     8161 ttgttattct ccgcaatcct aatctctaag ttaccgcatt tattgagaat ttctcctttt
     8221 tccctgtcgt ttttctccat aatttcttca taaaaaagcc gggctgcata aaagcaaacc
     8281 cggcctgatt gagataatga atagatttta ctgatgattc atcatcaatt tacgcaacgc
     8341 agcaaaatcg gcgggcagat tatgtgaaag caagggtaaa tcagcacgtt ctgccagctc
     8401 ttttggcaga tccaacgttt caccgagaat cgcttccacg ctctctttaa atttcgccgg
     8461 atgcgcggtg ccgaggaaca agccatattc gcctggattc aactggtcac acagcgcacg
     8521 atacgctacg gcagcgtgcg gctccgaggt gtagcccagt tcttttaact cacgcattgt
     8581 ctgttgcgtg gtttcatcat ccacggctgc ataacccagc tctttcagtt gccagatttt
     8641 gcggcggaac aactcttcca cacgcggcca gttgttcggc tgactcacgt ccatcgcgtt
     8701 ggataacgtc gcctgagtcg ctttgggtga ccactgaccg tcgtgcagga aacgtggcac
     8761 ggtatcgttc acgttggtcg cagcaataaa acgtttcacc ggcagaccga gtgacttcgc
     8821 cagcagaccc gccgtcaaat cgccgaagtt tccgcttggc accgagacaa ccagctggtt
     8881 gcgcgcttcc tgcggcagct gcgcaactgc ttcaaagtag tagcaaatct gcgccagcaa
     8941 acggctgatg ttaatcgagt tagccgagtt taaccccagc gccactttca gttcttcatc
     9001 atcaaacgcc tgcttcacca gcgcctgaca ggcatcgaaa tcgccgtcga tggcaacagt
     9061 ttcgatattg ccgcccaacg tacagaacag tttttcttgc agtggactga ttttgcctcg
     9121 tggatagagg ataaccactt tcacattcgg caaaccgtag aaagcatgag ccactgccgc
     9181 tccggtatca ccggaggtcg cggtcagaat ggtcactggc ttatcgcccg caatatgggt
     9241 cagcatttgt gccataaagc gaccgccgaa atctttaaat gccagcgttg gcccgtggaa
     9301 caattccaga caaccgacat cgctttcaac attggcgacc ggagccggga aggcaaacgc
     9361 cgcgcgcacg cgctcttcca ggatttcctg cgggatttca tcaccaataa acgccgagag
     9421 gatcttcgca ctgcgggtga caaaatccag cttcagcatc tcatcaattt cagtcaggct
     9481 gaattccggc aggtcatgcg gaaaaaacag cccctgattt ttgcccaacc cctgggttac
     9541 ggcttgcgca aagctgacct gctcgttgtg atctttcaga ttgtagagtt tcatttagtt
     9601 ttccagtact cgtgcgcccg ccgtatccag ccggcaaata tgaacaaaac cttcctgatt
     9661 ttgcaggtag ttcttaccca accagtcggc aacgcgctgg gcggtatccg gcttgtcaca
     9721 tagagcgaac aaggtcgggc cggagccgga gataccgctc gctaccgcgc cgatttccgc
     9781 gaccgcctgc cgcgcctgcc ggaagccagg cagtaaccgt tcacggtagg gttcagcgat
     9841 aacatctttc atcagcttcg cggcaagctc aggctgacgg gaatagcagg cgtgaatgaa
     9901 gccagccaga tgtcgcccgt gcgcaatgca atcctggcgg cgatactgcg ccggtaaaat
     9961 agcccgggct tctgccgtcg agactttaat tcccggatac gccagcaccc acagccactc
    10021 atcaaaccct ggcacttgct ggctgatgat gtcgttttct tcgatcatca actgcatacc
    10081 accaagaaaa cacggtgcca cgttgtcgta atgaatgctg ccggagatac gtccttccag
    10141 ctcgcccatc aaagccagca aacgggtgtc attaagtggc ttgccgcagt gttcattcat
    10201 cgccatcagc gccgcgacca ccgaacaggc gctggagcct aagcccgagc cgatcggcat
    10261 attcttttcc agagtcatcg ccactggaat ttgcttgccc agctccaggc aaaaacgctc
    10321 ccagcactga taaacgatat tttcccgtgg ttctgacggc agcttatcgg caaagcgtcc
    10381 gaggttgttg agactgaatg tctctgccgc ctcaaccgtg actacatctc cgagcaatgc
    10441 accatcaacg ggtgtcaccg ccgccccgag cacatcaaac ccgacgctca tattggcact
    10501 ggaagccggg gcataaactt taaccatgtc agactcctaa cttccatgag agggtacgta
    10561 gcagatcggc aaagacacca gcagcggtaa cgtcattgcc cgcaccatat ccgcgcagca
    10621 ccaacggcag cggctgataa tagtggctat aaaaggccag ggcgttttcg ccatttttca
    10681 ctttgaacag cggatcattc ccatccactt cggcaatctt cacgcggcag acaccatctt
    10741 catcaatatt gccaacatag cgcaaaactt ttccttcatc acgggccttc gccacgcgcg
    10801 cggcaaagag atcgtcgagc tgtgacagat tcgccataaa agcggcaaca tcaccctcgg
    10861 cgttaaactc tgcgggcagc acaggttcaa tttcaatatc cgccagctcc agttcacgtc
    10921 ccgtttcacg agcgagaatc aatagtttac gcgccacatc cataccagaa agatcatctc
    10981 gcgggtccgg ttcggtataa cccatttccc gcgccagcgt ggtcgcctcg gagaaactca
    11041 tgccttcgtc taacttgccg aagatataag aaagcgaacc tgaaagaatg ccggagaact
    11101 tcaccaattc atcacctgca ttgagcagat tttgcaggtt ctcaataacc ggtaatccag
    11161 ccccaacgtt ggtgtcatag aggaatttac gccgcgattt ttccgccgca taacgcaact
    11221 gatgatagta atccatcgac gaggtgttgg cctttttgtt cggcgtgaca acgtggaaac
    11281 cttcgcgcag gaagtcggca tattgatccg ccactgcctg gctggaggtg cagtcaacaa
    11341 tgaccgggtt cagcagatga tattctttca cgaggcgaat taagcgcccg agattaaacg
    11401 gctctttggc ttgtgcaagt tcttcctgcc agttttccag attaaggcca tgcacactag
    11461 tgagcagagc cttcgagttg gcaacgccgc agacacgtaa gtcgatatgt ttattcttca
    11521 gccagctttg ctgacgcttc agttgctcca gcagcgcacc gccaacgcca ccgacgccaa
    11581 tcacaaacac ttcgataacc tgatcggtat tgaacagcat ctgatgagta acgcgcacgc
    11641 cagtggtcgc atcatcgtta tttaccacga cagagattga gcgttcagaa gatccctgag
    11701 caatggcgac aatgttgata ttggcgcggg ccagcgcggc aaagaatttc gccgagatcc
    11761 cacgcaaggt gcgcatacca tcacctacca ccgagataat ggccagccgt tccatcaccg
    11821 ccagcggctc cagtaagcct tctttcagtt ccaggtagaa ctcttcctgc attgcccgtt
    11881 cagcccgcac acagtcgctt tgcggaacgc agaaactgat actgtattcg gaagatgatt
    11941 gcgtaatcag caccacggaa atacgggcgc gtgacatcgc ggcaaagacg cgcgccgcca
    12001 tgccgaccat tcctttcatc cccgggccgg aaacgctgaa cattgccatg ttattcagat
    12061 tggaaatgcc cttgaccggt aattcgcctt catcacgact ggcaccaatg agcgtacctg
    12121 gtgcttgagg atttccggta tttttaatca ggcaagggat ctggaactgg gcgatggggg
    12181 taatggtgcg ggggtgaaga actttagcgc cgaagtagga aagctccatc gcttcctggt
    12241 aggacatcga cttcaacaac ctcgcatcgg gcacctgacg cgggtcgcag gtatagaccc
    12301 cgtcaacgtc cgtccaaatc tcgcaacaat cggcgcgtaa acaggcagcc agcaccgccg
    12361 cggagtagtc ggaaccgttg cgtccaagta ccaccagttc gcctttttca ttaccggcgg
    12421 tgaagcctgc catcagcacc atgtgatcag ccggaatacg acttgccgca atacggcggg
    12481 tggactcagc aatatcgacg gtagattcga ggtaatgccc cactgccagt agtttttcga
    12541 ccggatcgat aacggtaacg ttgtgaccac gcgcttctaa cacgccggcc ataatggcga
    12601 tcgacatttt ctcgccacgg caaatcagcg cagcgttaat gctatccggg cactgcccta
    12661 acaaactaat gccatgcagg acatgtttta tttgggcaaa ttcctggtcg acgaaagttt
    12721 tcaattgcgc cagcgggaat cccggctggg cggcggcgag tcccgtcaaa agttcggcaa
    12781 aaatacgttc ggcatcgctg atattgggta aagcatcctg gccgctaatg gttttttcaa
    12841 tcatcgccac caggtggttg gtgattttgg cgggggcaga gaggacggtg gccacctgcc
    12901 cctgcctggc attgctttcc agaatatcgg caacccgcag aaaacgttct gcatttgcca
    12961 ctgatgtacc gccgaacttc aacactcgca tggttgttac ctcgttacct ttggtcgaaa
    13021 aaaaaagccc gcactgtcag gtgcgggctt ttttctgtgt ttcctgtacg cgtcagcccg
    13081 caccgttacc tgtggtaatg gtgatggtgg tggtaatggt ggtgctaatg cgtttcatgg
    13141 atgttgtgta ctctgtaatt tttatctgtc tgtgcgctat gcctatattg gttaaagtat
    13201 ttagtgacct aagtcaataa aattttaatt tactcatggc aggtaaccag ttcagaagct
    13261 gctatcagac actctttttt taatccacac agagacatat tgcccgttgc agtcagaatg
    13321 aaaagctgaa aaatacttac taaggcgttt tttatttggt gatatttttt tcaatatcat
    13381 gcagcaaacg gtgcaacatt gccgtgtctc gttgctctaa aagccccagg cgttgctgta
    13441 accagtcgac cagttttatg tcatctgcca ctgccagagt cgtcagcaat gccatgactc
    13501 gttcgcgtaa agcttgcagt tgatgttggt ctgccgttgt gtcacttttc gccggttgtt
    13561 gtattaatgt tgctaattga tagcaataga ccatcaccgc ctgccccaga ttgagcgaag
    13621 gataatccgc caccatcggc acaccagtaa gaacgtcagc caacgctaac tcttcgttag
    13681 tcagcccgga atcttcgcga ccaaacacca gcgcggcatg gctcatccat gaagattttt
    13741 cctctaacag cggcaccagt tctactggcg tggcgtagta atgatatttc gcccgactgc
    13801 gcgcagtggt ggcgacagtg aaatcgacat cgtgtaacga ttcagccagt gttgggaaaa
    13861 ctttaatatt atcaataaca tcaccagatc catgtgcgac ccagcgggtg gctggctcca
    13921 ggtgtgcctg actatcgaca atccgcagtt cgctaaaacc catcgttttc attgcccgcg
    13981 ccgctgcccc aatattttct gctctggcgg gtgcgaccag gataatcgtt atacgcatat
    14041 tgccactctt cttgaccaaa taaccgcgaa cagggtgatc actgtcaact tattacgcgg
    14101 tgcgaattta caaattctta acgtaagtcg cagaaaaagc cctttactta gcttaaaaaa
    14161 ggctaaacta tttcctgact gtactaacgg tttaattgtt aaaaaatgct acatatcctt
    14221 ctgtttactt aggataattt tataaaaaat aaatctcgac aattggattc accacgttta
    14281 ttagttgtat gatgcaacta gttggattat taaaataatg tgacgaaagc tagcatttag
    14341 atacgatgat ttcatcaaac tgttaacgtg ctacaattga acttgatata tgtcaacgaa
    14401 gcgtagtttt attgggtgtc cggcccctct tagcctgtta tgttgctgtt aaaatggtta
    14461 ggatgacagc cgtttttgac actgtcgggt cctgagggaa agtacccacg accaagctaa
    14521 tgatgttgtt gacgttgatg gaaagtgcat caagaacgca attacgtact ttagtcatgt
    14581 tacgccgatc atgttaattt gcagcatgca tcaggcaggt cagggacttt tgtacttcct
    14641 gtttcgattt agttggcaat ttaggtagca aacatgcaga ccccgcacat tcttatcgtt
    14701 gaagacgagt tggtaacacg caacacgttg aaaagtattt tcgaagcgga aggctatgat
    14761 gttttcgaag cgacagatgg cgcggaaatg catcagatcc tctctgaata tgacatcaac
    14821 ctggtgatca tggatatcaa tctgccgggt aagaacggtc ttctgttagc gcgtgaactg
    14881 cgcgagcagg cgaatgttgc gttgatgttc ctgactggcc gtgacaacga agtcgataaa
    14941 attctcggcc tcgaaatcgg tgcagatgac tacatcacca aaccgttcaa cccgcgtgaa
    15001 ctgacgattc gtgcacgcaa cctgctgtcc cgtaccatga atctgggtac tgtcagcgaa
    15061 gaacgtcgta gcgttgaaag ctacaagttc aatggttggg aactggacat caacagccgt
    15121 tcgttgatcg gccctgatgg cgagcagtac aagctgccgc gcagcgagtt ccgcgccatg
    15181 cttcacttct gtgaaaaccc aggcaaaatt cagtcccgtg ctgaactgct gaagaaaatg
    15241 accggccgtg agctgaaacc acacgaccgt actgtagacg tgacgatccg ccgtattcgt
    15301 aaacatttcg aatctacgcc ggatacgccg gaaatcatcg ccaccatcca cggtgaaggt
    15361 tatcgcttct gtggtgatct ggaagattaa tcggctttac caccgtcaaa aaaaacggcg
    15421 ctttttagcg ccgtttttat ttttcaacct tatttccaga tacgtaattc atcgtccgtt
    15481 gtaacttctt tactggcttt cattttcggc agtgaaaacg cataccagtc gatattacga
    15541 gtcacaaaca tcatgccggc cagcgccacc accagcacac tggttcccaa caacagcgcg
    15601 ctatcgacag agttgagcag tccccacatc actccatcca gcaacaacag cgcgagggta
    15661 aacaacatgc tattgcgcca gcctttcaat accgcctgca aataaatacc gttcattaac
    15721 gccccaatca gactggcgat tatccatgcc acggtaaaac cgatatgttc agaaagcgcc
    15781 agcaagagca aataaaacat caccaatgaa agccccacca gcaaatattg cattgggtgt
    15841 aaacgttgcg cggtgagcgt ttcaaaaaca aagaacgcca taaaagtcag tgcaatcagc
    15901 agaatggcgt acttagtcgc ccggtccgtt aattggtatt gatcggctgg cgtcgtcact
    15961 gcgacgctaa acgcgaggaa gttttcccag ccggtatcat tgcctgaagc aaaacgctca
    16021 ccgagattat tagcaaacca gctgctttgc cattgcgcct gaaaacctga ctcgctaact
    16081 tcccgtttgg ctggcaaaaa atcacctaaa aaactgggat gcggccagtt gctggttaag
    16141 gtcatttcgc tattacgccc ggcaggcacc acagaaagat cgccggtacc gcttaaattc
    16201 agggccatat tcagcttcag gttctgcttc cgccagtccc cttcgggtaa agggatatgc
    16261 acgccctgcc cgccttgctc taacccggtg ccgggttcaa tggtcagcgc cgttccgtta
    16321 acttcaggcg ctttcaccac accaatacca cgcgcatccc cgacgctaat cacaataaat
    16381 ggcttgccaa aggtgatatt tggcgcgtcg agttcgctaa gacgcgaaac atcgaaatcg
    16441 gcttttagcg ttaaatcact gtgccagacc tgaccggtat aaatccctat cttgcgttct
    16501 tccacgttct gattgccatc aaccatcaat gactcaggta accaaaaatg gataaaactt
    16561 cgtttccgct ccacggtttt atcctcttcc tgtaccgtat aaagctcggt cacaggaata
    16621 gcgataagcg gcccaacaag tttttgcggc ccgctggtac tttggcgaat cgcatcttcc
    16681 acatcgctac ggtaatcggc acgttcgaca atcacctgcc gaatcagcat tatcggaatc
    16741 aacaacagca atactgcacc aaacagggtg gtcattttcc agaacaggga ggatttcaac
    16801 atagtcttct cctttgcaat ggcagcagga tacgaaggct atgtgggaag aatttgaagc
    16861 tatgtgaagt gacggtgaag tcgaagcgag gccagcacgc caccttcctg cacgttgcgc
    16921 agcgtgactt cgccgttaaa caaacgggcg acctcactga cgaacgccaa ccccagaccg
    16981 ctgctttttt gcccatttgc acgcggcaaa gagtaaaagc gttcaaaaat acgtgacagc
    17041 gcgtagtcag gaataccact accggtatcc agcaccttaa gcgtgacgtg ttcctgatcc
    17101 acttcggcgc ttagcgttat acgaccgctc tcgggggtaa aatcgatggc gttatccagt
    17161 aaattcccca gcgcctgatc cagtaacgcc ggttcagcag caacattaac ctcggtgggc
    17221 gtaacatgta gagtgatgtt tttttctgcc aactgcacgg tgcgcgcttc gctgacgcgg
    17281 cggaataatg ccgccacatc aacagcagtc aaaacgactt cctgacgatt ctccagtctt
    17341 gcctggcgta gtaacgtttc caccagtgcc tgcattcgcg cattttgcgt cagaatgttg
    17401 tcagtaaaac gagccactac ttcaggcggc ggaccttcgc gtaaaatttc cgccgcgcca
    17461 cgaatcgccg ccagtgggct ttttagctca tgggttaacg cataaacata ctgctcaata
    17521 tagtttttcc cttccagctt cacgcgcata ctttccagcg cctgcgcgag tttacgcagc
    17581 tcgctactac cgagatcggg aagaggaacg ggcttattgt cagtgacgga atcagcatag
    17641 cgagtgagcc gggcaatgga gcggttgatc caccaaacca tgcctgcgcc aatcaccagt
    17701 gcgatcccca acaaaatggc gctggcccat aaaattcgcc gctcgctgcg tttaatgacc
    17761 ggagccatcg ccgcgttcgg tttgcctacg ctcaaaacgc caataagccg cgagccgtcc
    17821 ataatcggcg cggcaacata catcaccgaa ctttcgggat cggcaggatt ttgcaacgtg
    17881 ctacgcgcac catactgacc acgcaacgtt agccagacgt cattccagcg cgaataatcc
    17941 tgcccaacgg ctttatttgc cgaatcgaac aatactttgc cctgcgcatc ggtcatatag
    18001 acatgatatt cattgcgcac tttgttaatg ccaccgatat tggcgcgaaa cgggcgatgt
    18061 tgtagctgat taaacgcctg cgccagttgc ccatgcgttg ggtccccaga gagcaaatcg
    18121 ggacgcgcca gctccgccag caacgttgcg gtatcgatta acgtcccctc cgttgctctt
    18181 cgcacgcccg gtttaacttc tttgacaaaa atagccagta cgaaccaggc cgccaccgcc
    18241 accagtaaaa aatagcccag cagcaaccgc atgccgatac gcattacagg cccctcaggc
    18301 tatatcccat gccgcgatga gtattaatcg gagaaagatc ggggttgatg gcgcgcagct
    18361 tggcacgcaa cgttttaatg tgggtatcga cggtgcgatc gtaggtgtcc tgcgcatctt
    18421 cccacacgct atccattagt tgctggcggg accagacgcg gcctggcgac ttgagtaacg
    18481 tcttcagcaa taaaaactca taccgcgtca gcgttaatgg cgtgtcaaac cagctgatct
    18541 gcgccgcggg ttcattcaat tcaaaatgac caatacggat gacgggagac ggcgacgaga
    18601 acttcttcac ccgacgcagt aaggtgcgca ccctggcgca cacttcgcgg ggtgaaaacg
    18661 gtttagccac atagtcgtca gcgccaattt ccagcccaag caggcgatcg acctcttcac
    18721 ttcgggccgt caggaacagt acaggtaacg ccggatggag cgccagtaac tggcggcaca
    18781 attcaaagcc gctgatatca ggcaaaccaa catcgagaat catgacgtcg ggaacctgct
    18841 ggcgagcttt atccagcacc ggcaagcctc gctcaaagac ctcgacggca aaaccttcct
    18901 gctgcaacat gtagaccagc gtgtcggcta tcccttgctc atcttccact aaccagaccg
    18961 tttcccgttg cataaatcgc ctctgttatt gccgccacgg catgacagga accgcgctaa
    19021 tcgcgttttt cggcgaacct tctacaactt tgtcggagta agccagataa gcgagcgcgt
    19081 tgcgtttggc gtcataaaag cgcacgacct gtaacgactt aaagaccagg gacgtgcgtt
    19141 ttttgaatac tacctcgccc tgagctttgc cgtttttaat acgatccgac agttcaatcg
    19201 gcccgacttg ctgacaagaa atagccgcat cggaggtatc ttccgccaga cccaatcccc
    19261 ctttaatacc acctgttttc gcccggctca cataacaggt gacatttttc acatcgggat
    19321 cgtcaaaggc ttccacaaca attttgtgat ccgggccgat cattttaaat acggtgtcga
    19381 ccgaaccaat ctcttctgca tgagccaatg gccccagcat tatcaggctt aaagacagga
    19441 tcaaatgctt gtatttcata ttgttaccat ttttatctat tacacgagca attagttcgt
    19501 cacggtaaat aacatattga ttaagaacaa aatctcaata cttttatttc cgtcaagccc
    19561 taaaacatac tctactaaga aaaaaacact gaatgctaaa acagcaaaaa atgctattat
    19621 ccaattacct gatgtcaggt gctcgttgtt gaaaggatga ggatatttta tggatcaggc
    19681 cggcattatt cgcgaccttt taatctggct ggaaggtcat ctggatcagc ccctgtcgct
    19741 cgacaatgta gcggcgaaag caggttattc caagtggcac ttacagagaa tgtttaaaga
    19801 tgtcactggc catgctattg gcgcgtatat tcgtgctcgt cgtttgtcga aatcggcggt
    19861 cgcactacgc ctgactgcgc gtccgattct ggacatcgcg ctgcaatacc gcttcgactc
    19921 tcaacagaca tttacccgcg cattcaagaa gcagtttgcc cagactcctg cactttaccg
    19981 ccgttctcct gaatggagcg cctttggtat tcgcccgccg ctacgtctgg gtgaattcac
    20041 aatgccagag cacaaatttg tcaccctgga agatacgccg ctgattggtg ttacccagag
    20101 ctactcctgt tcgctggagc aaatctctga tttccgccat gaaatgcgtt atcagttctg
    20161 gcacgatttt ctcggcaacg cgccgaccat tccgccagtg ctctacggcc tgaatgaaac
    20221 gcgtccgagt caggataaag acgacgaaca agaggtattc tataccaccg cgttagccca
    20281 ggatcaggca gatggctatg tactgacggg gcatccggtg atgctgcagg gcggcgaata
    20341 tgtgatgttt acctatgaag gtctgggaac cggcgtgcag gagtttatcc tgacggtata
    20401 cggaacgtgc atgccaatgc tcaacctgac gcgccgtaaa ggtcaggata ttgagcgata
    20461 ctacccggca gaagacgcca aagcgggaga tcgcccaatt aatctgcgct gtgaactgct
    20521 gattcctatc cgccgttaac gctgcagctc atctaatgca ggggcgtcca gatgcgagat
    20581 gtcccctgcg gtttcaacga cccagccaga cgccagccac aggctttcct gataatccac
    20641 gcgcgaaata gaacagttac gcagacgtaa gcggcgctct gcccatgctg gtaatccgag
    20701 aatcgtactc accagacatc ccagtgcaat accatggctc accagcagcg gtcggcttcc
    20761 ctgcggtaag tcccggcaag attccagtgc cgcattgacg cgatcgctga gctcctgcat
    20821 tgactcgcct tcaggaatac gcccgtcaac ggtgccattg accagctgcc gacgccagtt
    20881 ctcttcttct tcggtcagag aatcgatatg tcttttttcc agcacaccca tgtttaattc
    20941 acgcaggcga gaatcaaaga tgatgtcaca gccgcaggcc tgggcgatga tttccgccgt
    21001 acgccgggtg cgtcctaaat cgctactgat aatatgcgta atgccaagct ctttggcacg
    21061 ggttgccacc tgcatcgcct gttgctcacc tttggcggtc agcgggctgt cagactggcc
    21121 ctgaatacgt cgctcggcgt tccactgcgt ttcaccgtgg cggactaggt atacctgtaa
    21181 catgcttttt ttccgtatac tgctgtaacg ttaattttct gagtaatgct gattatgcac
    21241 caagttatct gtgcgaccac caatcccgct aaaattcagg ccattctgca ggcatttcac
    21301 gagatcttcg gcgaaggatc ctgccatatt gcatccgttg ccgtcgagag cggtgtaccg
    21361 gaacagccct ttggcagtga ggaaacgcgc gctggcgcac gaaatcgggt agcaaatgcc
    21421 cgccgtttac ttccagaggc tgatttttgg gtggcgattg aagctggcat cgatggtgac
    21481 agcactttca gttgggtggt gattgaaaac accagccagc gcggcgaggc gcgttcggct
    21541 actttgccgt tacctgcggt gattctgcag aaagtgcgtg aaggtgaggc gcttggtccc
    21601 gtgatgtcgc gttataccgg tattgatgag attggtcgta aggaaggggc gatcggcgtg
    21661 tttaccgccg ggaaactcac tcgcgccagc gtctatcacc aggcggtgat cctggcgctg
    21721 agtccgtttc acaatgccgt gtatcaggca ttgtaggacg gataaggcgt tcacgccgca
    21781 tccggcattc ggtgcacgat gcctgatgcg acgctgccgc gtcttatcag gcctacaaaa
    21841 tcaatcgctt ttcagcaaca cctcttccag ccactggcgc agctccacgg gcgcggcttt
    21901 caggctgtta gatccacgcg taatcgtcgc gatgcccgcg ccgagttcat tttttaactc
    21961 acgctgactc atttcgccac gcaacagctc ttcgacaata cgcacgcgag tccccaacgc
    22021 ttcgcgctca tctggcgtca gcatcaggtt taacaacggt aaatggagat cgttttggta
    22081 ggcattctta agcaggtcga caaaacgtaa ccactcctgg tgacgctgtt ctgccatcgc
    22141 tgctgaatag ggtgattgtt gggccataat atgtcgccat tgttagcggg ggaagcaaaa
    22201 tgcctccccc ggttgtactc gctaaagagt acgatagcat atcataaacg tgcggatcag
    22261 taacgacgtc cccattccgt ggcgctcatc aacgtcggtt tatcccccat gaaataacgg
    22321 tagtaagcgt cataagccag cacgttcttc acataaccgc gcgtctcgga gaacggaata
    22381 ctctcgacaa atgccacagc gtcgatacgc ccggcgctgt tgccaagcca ggttcgcacc
    22441 cgtccaggtc cggcgttata agctgctgag gagaaaatac ggttattgcc aaactgctga
    22501 taaacatatt gcaggtaact ggtgccaatg ttgatattcg tttccggatc cagcaattgc
    22561 ccagggctgc tataaccagg aatagagaac atcttcaccg tatgggtcgc tgtaccaggc
    22621 ataatctgca tcaggccgct ggcccctacc ggtgatttca ctttcggatt ccaggcgctc
    22681 tcctgacggg caatcgccat cgcatagctt tgcgggatct ctttcccact ggtgtaacgt
    22741 ttgaaaagat cgttgtaagc cagcgggaat cgctcttcca gatgatccca aagcttcccg
    22801 gcgatcgttg cctgaacgct aagatcccac cattggttat tgaaagcata ccgagccagt
    22861 tgagcctgct ctgtttttga cttgctcttc accagattgg cccactcgct acgcgcggtg
    22921 ttatcgagat tccagtacat caactcgcgc acgcgcgcca tctccggccc ctgagtcagg
    22981 gcgctgtcaa cattctgcgg cgctttatca atcttcagct catactcttc tccgatgcgt
    23041 tgtgctgcaa ccatcgggta gaaaccacgc tgttgcatga gttgatgcaa aatctctttc
    23101 gcttcagctt cacgtccgcg ttccagcaat aaatccacct gccagtaacg ccattcatct
    23161 ttctcttttg cttccatcgg cagacgcgcc agccaggtat tcaggccgcg acgatcgccg
    23221 gtgccaagtg ccattcgcac acggcgttca atcaacgacg tggattgcga gcgcataatg
    23281 acgtcatcgc gccatttcgc ctgctcgtcg gtgacatcgt tgcccatcaa acgccaggcg
    23341 acgatatcgc gcagctcctg aatctgatct tcattaagct gctgcacctg ggcaagcgac
    23401 gggatcatca gccgtgcatt ttcagcatcc tgccgcgcca cactggcaaa cgccaccgct
    23461 gccatttgac gggtaaaatc ggtcgctccg gttgtacgcg cgaaggtcag taccgtatta
    23521 gggttgttcg ccagtgaaat gattgccgag gcgatagtct ggtaatcggc aggcatctgc
    23581 cctgccagca ctgttaccag gccagtgtta cccgctttca tcgccagacg gatacgctct
    23641 aaatacgcca gcggatcttg tttacctgac gcacgccaga cgctaaataa cttgtcacag
    23701 gcgttaggct ggctcttgcc ggttagccac agctctttcg ccccttgcca ggcttcttca
    23761 ctctgcccgg tgttccattt cgcatagtag taattacatt gcgcttcggt agttccgggc
    23821 ttttccgggc taaaggctaa caagccacgc cagtcttcac gccgcgccag ttcattgacg
    23881 aaacgagatt gcagcgtgcg agcgggagga agcgtggggt tagcgcgaac aaagttagtg
    23941 accgtcaccg ccggttgatt catcagatca tcggtgatct ggcggtattc caggtaggga
    24001 taaagcggat aatccttcag tccaggcatc atttgttcga ccacatccat ttgtcgatta
    24061 tcccaggcct gcttgatttg cgcgtaacgg ctacgctgct catccagtga gtcggctcgc
    24121 gccacgctgc tgaccgtcag cagacagaca ccggcagcca acagccgcca ggtaacttgt
    24181 ttggcttttt ccacaagcgc atcctctaag tgtaaataca tcaatgcagc gtcatgccgc
    24241 gtaatagatt tatgctaacc agtcattgcc gtttacgcca cgttacggac acttttttac
    24301 ttttactgcg aaggtgatcg accaactggg attaggcggg gaaaaaggct acacttcgcc
    24361 tttgaaaact catcatcacg ataaacatag aggcgaagtc caacgtggct caattcgttt
    24421 ataccatgca tcgtgtcggc aaagttgttc cgccgaaacg tcatattttg aaaaacatct
    24481 ctctgagttt cttccctggg gcaaaaattg gtgtcctggg tctgaacggc gcgggtaagt
    24541 ctaccctgct gcgcattatg gcgggcattg ataaagacat cgaaggtgaa gcgcgtccgc
    24601 agccagacat caagattggt tacctgccgc aggagccgca gctgaacccg gaacacaccg
    24661 tgcgtgagtc cattgaagaa gcggtttctg aagtggttaa cgccctgaaa cgcctggatg
    24721 aagtgtatgc gctgtacgcc gatccggatg ccgattttga caagctggcc gctgaacaag
    24781 gccgtctgga agagatcatt caggctcacg acggtcataa cctgaacgta cagctggagc
    24841 gtgcggcgga tgcgctacgt ctgccggact gggacgcgaa aatcgctaac ctctccggtg
    24901 gtgagcgtcg tcgcgtagcg ttgtgccgcc tgctgctgga aaaaccagac atgctgctgc
    24961 tcgacgaacc gaccaaccac ctggatgccg aatccgtggc ctggctggaa cgcttcctgc
    25021 acgacttcga gggcaccgtg gtggcgatta cccacgaccg ttacttcctc gataacgttg
    25081 caggctggat cctcgaactt gaccgcggtg aaggtattcc gtgggaaggc aactactcct
    25141 cctggctgga gcagaaagat cagcgcctgg cgcaggaagc ttcacaagaa gcggcgcgtc
    25201 gtaagtcgat cgagaaagag ctggagtggg tacgtcaggg aactaaaggc cgccagtcga
    25261 aaggtaaagc acgtctggca cgctttgaag agctgaacag caccgaatat cagaaacgta
    25321 acgaaaccaa cgaactgttt attccacctg gaccgcgtct gggcgataaa gtgctggaag
    25381 tcagcaacct gcgtaaatcc tacggtgatc gcctgctgat tgatgacctg agcttctcga
    25441 tcccgaaagg ggcaatcgtc gggatcatcg gtccgaacgg cgcgggtaaa tcgaccctgt
    25501 tccgtatgat ctctggtcag gaacagccgg acagcggcac catcacttta ggtgaaacgg
    25561 tgaaactggc gtcggttgat cagttccgtg actcaatgga taacagcaaa accgtttggg
    25621 aagaagtttc cggcgggctg gatattatga agatcggcaa caccgagatg ccaagccgcg
    25681 cctacgttgg ccgctttaac tttaaagggg ttgatcaggg taaacgcgtt ggtgaacttt
    25741 ccggtggtga gcgcggtcgt ctgcatctgg cgaagctgct gcaggttggc ggcaacatgc
    25801 tgctgctcga cgaaccaacc aacgacctgg atatcgaaac cctgcgcgcg ctggaaaacg
    25861 ccctgctgga gttcccgggc tgtgcgatgg ttatctcgca cgaccgttgg ttcctcgacc
    25921 gtatcgccac gcacatcctg gactaccagg atgaaggtaa agttgagttc ttcgaaggta
    25981 actttactga gtacgaagag tacaagaaac gcacgctggg cgcagacgca ctggagccga
    26041 agcgtatcaa gtacaagcgt attgcgaagt aatgcgtaaa atgccggatg cggcgtgaac
    26101 gccttatccg gcctacaaac caagaaattt caatacgttg tgaatctcca ggcctgataa
    26161 gacgcggtaa gcgtcgcatc aggcattgag caccgaatgc cggatacggc gtgaacgcct
    26221 tttccggcct acaacagcct tagtatccgc aatttcgtag cgggtcaccc gtgcaggttt
    26281 tttcaaacct tgtttaatct cgacagcctc ttccagagag gttttcaact catcaaaaaa
    26341 cttattttat acctcgcctt tgagccgttt catcgcggtt atctctgctc ccccatcatc
    26401 tcccgcacca gctccacgca gcgcaggaaa cggctgtcat aatcgtcctc ttcaacccgc
    26461 acgaattcga tattgttctc ttccagcatc tccaccagca agttctggaa ctctttgcga
    26521 tccaccgaac tgccgaggct gcgtaaacca tccgccaccc acggcgtgtt gttctccagc
    26581 aggatcacca gatcgaaacg gtattcatca atcaacgcct gtacgaacgg atgctcacgc
    26641 ccttcgtact ttttgcagaa cgcctgggta gtgacaaaat cagtgtcaat aaacgccact
    26701 ttattggcat atttcactgc aaaatcaatg tattgtgcgt ggcccagcgc gattttatcg
    26761 taatcggaat actgcaatgc gatctcatca ccgccgaggt gtgaaaagac ataatcgcga
    26821 ccatattccc acgcactggt ggtattgaag atattggcaa gtttgtttac cagggtggat
    26881 ttaccgctcg actcgccacc aaggatcgcc acggtacgta caaagaacgg cttcacttcc
    26941 gtaggaatat attcccagta gcggaacggg ttttcgcgga tctgcgcacc gctgatactc
    27001 ataaaggtac gtttcggatc gaccagcacc gtatcgatcc ccagatgttc catatactgt
    27061 ggcgcatcgg cttcttccga ggtgtagatc aggtccggct gaatcccctt ttcagccata
    27121 aactttttga tgccgttgct ccacacatcc cagccgtgcg gatacggctc catgccctct
    27181 tcgttgaaag catgaatgcg aatatttttc tgatatttaa aagtttgtaa taaccagcgc
    27241 agacgatccg gcaccgtggg ctgctgcgac atggcgctgt cttcaaacaa cgcgcggtcg
    27301 cgggtatcgt caaaacccat aatgatatgc agctcgtcaa cctggctaca ggcgcgctgg
    27361 ataaggtaga tatgtccggt atgcagcggg tagaacttac cgaatacgac accaatggtt
    27421 ttcttctgtc ggggaaattc aagccccaaa aaacggtgca acgcctccag cttttgcgcg
    27481 ctggggcttt taattttggc attcagtaac tggcttaaat accctttggt cataccgctg
    27541 gcatcagcta cctgctgtag cgtgcagccc tgttgcttga tggcagtttt caggtaatca
    27601 aatgacgaca tatctccctc cgtatctctc attataagtc gtcgaacacg ctaagcgcgt
    27661 cggagagttt tttaacgcca aaaatctgca tcccttccgg cgcttttttc ggtacgttag
    27721 ccgccggaac aatcgcccgg cgaaaaccgt gtttcgccgc ttctgaaatt cgttcctgac
    27781 cgctgggcac cgggcggatc tcccctgcca gcccgacttc accaaacacc accagatcct
    27841 gtggcagcgg tctgtcacgc aggctggaaa ccatcgccag cagtaacgct aaatcggcac
    27901 tggtttcggt taccttcacg ccgccgacca cgttcacaaa tacatcctga tcggccattt
    27961 gcagaccacc gtgacggtgc aagacagcca gcaggattgc cagacggttt tgttccagcc
    28021 ccactgccac gcggcgcgga ttcgccatca tcgagtgatc gaccagcgcc tgaatctcca
    28081 ccagcaacgg acgcgttcct tcccacacca ccatcacgga gctgccggag gtcacttcat
    28141 ctccgcgact taagaaaatt gccgaagggt tgctgacttc acgcagcccc tgttcggtca
    28201 tcgcgaagac gcccagctca ttcaccgcgc cgaagcggtt tttatggctg cgcaaggtgc
    28261 gaaaacggga gtcggcatca ccatccagaa gcaccgaaca gtcgatacag tgttccagca
    28321 ctttagggcc agccagcgag ccatctttgg ttacgtgccc aaccatgaca atcgccacac
    28381 cgcgcgtttt ggcgaagcgc gtcagataag ccgccgtttc acgcacctgc gccacgctac
    28441 caggcgatga ttgtacatcc gccatatgca tcacctggat cgagtcaatt accatcagtt
    28501 tcggttgctc ttcttcggca atcaggcaga tctgttcgat gctggtttcc gacaacatat
    28561 tgagattgtc agtcggcagg ccaaggcgat gagcgcgcat tgccacctgt tgcagcgact
    28621 cttcgccagt gacatacagc gttttcatct gctgggccag tttgcacagc gtttgcagta
    28681 gcagcgtgga tttccccgca cctgggttac cgccaatcag aatggcgctt cccggcacca
    28741 cgccgccgcc tagtacgcgg tcgaactctt taaatccggt ggaaaaacgc ggcagctctt
    28801 caaggctgat atcggagagt ttctggactt ttgccacccc ggcgctaccg gcatagccgc
    28861 tgagacgctc gttacgcgcc accgttggcg acgcagcaag acgcacctcg gtgatggtgt
    28921 tccaggcatg acaggcactg cactgcccct gccagcgcgg ataatcggcc ccgcattcat
    28981 tacaaacaaa ggcgcgtttt ggagcttttg ccacggtgta cctcgttaat gctgtgccgc
    29041 ccgcaggatg gcgggcgagc aattacttct gattcaggct gccagagagg atgcagaata
    29101 cccccatcag gtcagcgtga cggatggtga cttccgcctt ttcattcact tttggcttgg
    29161 catggtaggc aatccccagc cctgctgctt tgatcatcgg caggtcatta gctccatcgc
    29221 caatcgccac ggtctgcgcc agcgggattt catactcctg cgcgaggcga gtcaaagttt
    29281 tcgctttgta ctgcgcgtct acgatgtcgc cgatcacatt gccggtaaat ttaccgtcca
    29341 tgatctccag ttcattggct accacggcgg tcaggcgcag cttgtcgcgc agatattcag
    29401 caaagaaagt aaagccgccg gaggcaatcg ccactttcca gcccagcgtt tccagcttga
    29461 gcaccagttg cgttaagcct ggcatcagcg gcagattttc acgcacctgt tgcagaatat
    29521 tggcgtcagc gcctttcagc gtcgccacac ggctgcgcag gctggcggta aaatcgagtt
    29581 cgccgcgcat cgcccgttcg gttacttccg ccaccatctc gcccgttccg gccagtttgg
    29641 caatttcatc aatacattca atctggatgg cggtggaatc catgtccatc accagcaaac
    29701 ccggcgtgcg caggtgcggg attttcccca gcggggcgac atccagttgc gcttcatggg
    29761 ccaggcgcgt agcccgtgcg gtgagtgaac ctgccagacg aatcacctga taatcttcca
    29821 cgcaccaggc ggcaacaatc accatcgccg cacccagttt gctctggtat tgggtcagac
    29881 gctgtttatc cagcccacga ccatacagca gccagccgct acgacctgcg tggtaatcca
    29941 gtggcatcac ttcatcacca cttaatgaaa gaggcagacc cggccataaa gagacatctt
    30001 caggcaggtc gcaccaggta atgttaggca ttaaggctcc tgtaaaatcg ttcgaagcag
    30061 ggaaaataac gcatgaggct accttgtatc cattgcttct ggcaacatta agtctcaaat
    30121 tttcaaaggg tggaagatgg ctcgcacaaa actgaaattc cggctgcatc gggcagtgat
    30181 tgtcctgttc tgtcttgcct tgttagtggc actgatgcag ggggcgtcat ggtttagtca
    30241 aaaccaccag cgacagcgta atccacagct ggaagaactg gcccgcaccc tggcgcgtca
    30301 ggtgacgctg aacgttgcac cgctgatgcg taccgactca ccagatgaaa aacgcattca
    30361 ggcgatcctc gatcagttaa cggatgaaag ccgcatcctc gacgcgggtg tgtatgacga
    30421 acaaggcgat cttatcgccc gttcaggcga aagcgtcgaa gtgcgcgacc ggctggcgct
    30481 cgacggtaaa aaagcaggcg gctattttaa ccagcagatt gtcgagccaa ttgcgggtaa
    30541 aaacggaccg ctcggctatc tgcgcctgac actcgacacc catacgctcg ccaccgaagc
    30601 ccaacaggtg gataacacca ctaacatttt acgcctgatg ttgctgctct cactggcaat
    30661 cggcgtagtg ctgacccgca cgctgctaca gggtaaacgc acccgctggc agcaatcgcc
    30721 cttcctgtta accgccagca aaccggtgcc ggaagaggaa gaaagcgaga aaaaagagtg
    30781 acccattact acaagaaagg aaatcgttat gtccacatta cgcctgctca tctctgactc
    30841 ttacgacccg tggtttaacc tggcggtgga agagtgtatt tttcgccaaa tgcccgccac
    30901 gcagcgcgtt ctgtttctct ggcgcaatgc cgacacggta gtgattggtc gcgcgcagaa
    30961 cccgtggaaa gagtgtaata cccggcggat ggaagaagat aacgtccgcc tggcgcggcg
    31021 cagtagcggt ggcggcgcgg tgttccacga tctcggcaat acctgcttta cctttatggc
    31081 tggcaagccg gagtacgata aaaccatctc tacgtcgatt gtgctcaatg cgctgaacgc
    31141 gctcggcgtc agcgccgaag cgtccggacg taacgatctg gtggtgaaaa cagtcgaagg
    31201 cgaccgcaaa gtctcaggct cggcctatcg cgaaaccaaa gatcgtggct tccaccacgg
    31261 cactttgctg ctcaatgccg accttagccg cctggcaaac tatctcaatc cggataaaaa
    31321 gaaactggcg gcgaaaggca ttacctcagt gcgttcccgc gtgaccaacc tcaccgagct
    31381 gctgccgggg atcacccatg agcaggtttg cgaggccata accaaggcct ttttcgccca
    31441 ttatggcgag cgtgtagaag cggaaatcat ctccccggac aaaacgccag acttgccaaa
    31501 cttcgccgaa acctttgccc gtcagagtag ctgggaatgg aacttcggtc aggctccggc
    31561 attctcgcat ctgctggatg aacgctttag ctggggcggc gtggaactgc atttcgacgt
    31621 tgaaaaaggc catatcaccc gcgcccaggt gtttaccgac agcctcaacc ccgcgccgct
    31681 ggaagccctc gccgggcgac tgcaaggctg cctgtaccgc gcggatatgc tgcaacaaga
    31741 gtgcgaagcg ctgttggttg acttcccgga ccaggaaaaa gagctacggg agttgtcgac
    31801 gtggatagcg ggggcggtaa ggtaactcgc tgattaaaat accaaaggaa gggaatatga
    31861 tcgatctgga aaaccaggag cgggagatta tcaatttaat gttaagccag cgtatttcct
    31921 ggctagccgc cgtgcgtatc aggcataaat tatcgcttgc tgaagtaagt aaaatgttag
    31981 gtatctcgat taattctcta aaatagatcg aaaagacgga acggcttagc tcaaacatca
    32041 aaagcaaaat gggagaaatt tatggttgtc caccagagtt gctgatatgc ccttcctgga
    32101 tgacggctga acataaatga atagattcgt gaatagatac ttgaatgtat tttatttttt
    32161 atccatatga aaaataatag taaatctata aatcagttgc tattgatgaa tagatactaa
    32221 aaagccggag cagtccccca ctccggctta acacgacaat cgcctttcag cgaaacacaa
    32281 ttactcttta tcgcccagca gaacggattc cagtgcgatt ttgatcatgt cgttgaaggt
    32341 ggtctgacgc tcagcggcag tggtctgctc gtgagtgcgg atgtggtcag acacagtgca
    32401 gatggtcagg gctttcgcgc caaattctgc agcgacgccg tagataccag ccgcttccat
    32461 ttccacgccg aggatgccgt atttttccat cacgtcgaac atttcgccgt ccggagagta
    32521 gaacaggtca gcggagaaca ggttaccaac gcgagcatca atgcccagtg ctttagccgc
    32581 gtctaccgcg ttacgcacca tgtcaaagtc agcgatagcg gcaaagtcat gatctttaaa
    32641 acggatgcgg ttaactttgg aatcggtgca ggcacccata ccgataacga cgtcgcgcag
    32701 ttttacgtgc ggcagaactg cgccacagga acccacgcgg ataattttct tcacgccgaa
    32761 atcggtgatc agttctttgg tgtagatgga gcaggacggg atacccatac cgtgacccat
    32821 tacggaaatt ttgcggcctt tgtaagtacc ggtgaagccc agcataccgc gaacgttgtt
    32881 cacttcacgg gcatcttcaa ggaaagtttc agcaatatac ttcgcacgca gcgggtcgcc
    32941 tggcatcaaa actacgtcag cgaaatcgcc catttctgca ttaatgtgtg gggtagccat
    33001 tgttttatcc ttatcaacat gttttcaccc tctccctgag ggagagggcc ggggtgaggg
    33061 catcaagccg cacccattat cagaacatgg ctttgccata ttccatatca gaagtaccaa
    33121 aatattttgc cagagtctgg ccgatatccg cgaaggtttc acggtgaccc agtgagcccg
    33181 gttttacttt cgggccgtaa accagtaccg gaatgtgttc acgcgtgtgg tcagtaccgg
    33241 tccaggtcgg atcacaaccg tggtcagcag tgaggatcag gatgtcgtca tcgcgcagca
    33301 gagacatcag ctccggcaga cggcggtcga acagctccag acccacagca taaccggcga
    33361 cgtcgcgacg gtggccccag gaagagtcga agtcaacgaa gttggtgaag acgatggtgt
    33421 tatcacccgc ttctttcatc tctttgatgg tggcgtcaaa cagcgcgtcc aggccagttg
    33481 ctttcacttt cttggtgata ccgcagttgg cgtagatgtc tgcaatttta ccgacagaaa
    33541 ccacctgacc gtgtttttca tcaaccagtt tctgcagcac ggtcggtgct ggcggctcaa
    33601 cagccaggtc gtgacggtta ccggtacgct ggaagttacc ggctttgtca ccgataaacg
    33661 gacgagcgat aacacgaccg atgttgtagc cgccgttggt cagctcttca cgggcgattt
    33721 cgcacagttc gtagagttta tccaggccaa aggtttcttc gtggcaggca atctggaaca
    33781 cagagtcagc ggaggtatag aaaatcggct tgccggtttt catgtgctct tcgcccagct
    33841 gatccagaat gaccgtaccg gaagagtggc agttaccgag gtaacccggc agattagcgc
    33901 gttcaaccag tttatccagc agctcttgcg ggaagctgtt ttcgtgatcg gagaaatatc
    33961 cccactcaaa tagaaccggg acaccggcga tttcccagtg accagacggg gtatctttac
    34021 cggatgacat ttcgtgcgcc catgcgtacg cgccgataac ttcagcgttg ccgtccattc
    34081 ccgccggaat gaaaccggta gaaccttcgt gtgctttcgc cagccccaga cgggtcagat
    34141 ttggcagatt gagcggacct ttacgaccgt tatcagcttc gcctttggca caagcttctg
    34201 cgatatgacc cagggtgtca gccccgacgt caccaaagcg ttctgcatct tctgtagcgc
    34261 cgatgccgaa tgagtccagc accataataa atgcacgttt catatgttct ccgtactcag
    34321 tgcttcaaat ttaaatgatc agatcagtat accgttattc gctgatacgg cgatagacag
    34381 ttggtgtgct ttccggtgct ttatcggcaa gtttaattgc cgctttcacc gctttcgccg
    34441 cttcctgcca gctgttttcg tctttcgcgt ggataaccgc cagcggacgc tgaccgtcta
    34501 cctggtcgcc caaacgcgcc atatcagtaa agccgacgct gtaatcaatg gtgtcagatg
    34561 cctgacgacg accgccgccc attgcaacca ctgccatccc cagcgcgcgg gtatccattt
    34621 cactgacaaa accttcggta tcagcataga ctgctttcgt cagcatcgct gtcggcagat
    34681 acttcgcgta gttctcaacg aagtcggttg ggcctttttg tgccgctacc atacgaccaa
    34741 agacttctgc tgctttaccg ttgtccagca ctgcttgcaa tttcgcgcgc gcttcggcgt
    34801 catctttcgc cagtttgccg gagataagca tctccacgca cagcgccatc gtgacatcaa
    34861 acagacgcgg gttacgatac tcacccgtca ggaactgcac cgcttcacga acttcaaccg
    34921 cgttacctgc actggaggcc agtacctgat tcatgtcggt gagcagcgcg gtggtgcgca
    34981 cgccagcgcc gttagccacg ccaacaatcg cttcggcaag ggcttcagag agttcgtagg
    35041 tcggcataaa cgcgccgcta cccactttca cgtccatcac cagcgcgtcc agaccttccg
    35101 caagtttctt cgccagaata gaggcggtga tcagcgggat ggagtccacg gttgcggtaa
    35161 tatcacgggt cgcgtagaaa cgtttatccg ccggagccag tgagctggtc tgaccgataa
    35221 tcgccacgcc gacgtcttta ataatttcgc ggaaacggtt gtcatccggg aaaatgtcga
    35281 agccagggat ggattccagt ttgtcgagcg taccgccagt atgaccgagg ccgcgaccag
    35341 agatcatcgg aatatagccg ccgcaggctg cgaccatcgg ccccaacatc agcgaagtca
    35401 catcgccgac gccaccagtg gaatgtttat caacaatcgg gccattcaga tgcaggcttt
    35461 tccagtcgag aacggttcct gaatctcgca tcgccatggt cagcgagaca cgctcaggca
    35521 ttgtcatatc gtggaagaaa atggtcatcg cgagggcggc aatctgccct tcggagatag
    35581 tgttgtcgcg aatgccgttg ataaagaaac gaatttcttc atcgctcagt gcatgaccat
    35641 cacgtttttt acgaataatt tcttgtgcga gaaacaaggt aacctcccga ggaaaagagt
    35701 atgaggcggc ttttgccgga tgcgtctacg ccttatccgg cctacgggta gatacgtctc
    35761 gtaggcctga taagcgcagc gcatcaggcg taaagcatct tacttagtag ctgctggcgc
    35821 tcttaccgtc accgtggccc agcgctttca acaggcttgc cagcaggctg gaagcaccaa
    35881 agcggtagtg acgcgcatct gcccagtcag caccgaacag ttcatctgcg atggcgagat
    35941 atttctgcgc atcttccgca gtacgcacgc cgcccgctgg tttgaaacca acggtttttt
    36001 ctacgcccat atcacggatc acttccatca tgatgcgcgc gctttccggc gtcgcgttca
    36061 cagccacttt accggtagag gttttgatga agtccgcacc cgctttgatg gagatttcag
    36121 acgctttacg gatcagcgct tcgtctttca gttcgccggt ttcgatgatc actttcagca
    36181 gtacattcgc tgccgcgcaa gcctctttac aggctttcac caggtcaaaa ccaacctgct
    36241 cgttacccgc catcagcgcg cggtacggga acacaacgtc aacttcatca gcaccgtagg
    36301 cgattgccgc acgggtttct gccagcgcga tttcgatgtc atcgttaccg tgtgggaagt
    36361 tggttaccgt agcgattcgg atttccgggg tgccctgctc tttcagcgtt ttgcgagcaa
    36421 tcgggataaa gcgaggatag atacagatag cggcggtatt gccgaccggg gttttggcct
    36481 gatgacacag agcaattact ttctcgtcgg tgtcgtcgtc attcagggtg gtcaggtcca
    36541 tcaatttcag tgcacgcagg ctgcttgctt tcagatcagt catttcattc tcctggcttg
    36601 tcgccaataa aattcacctt gcgagtttgt tagtattcta acatctactc cgcaacacac
    36661 ttcgatatac atcacaatta aggaaatcta cttacaagtt tgcatcactg taatgcgatc
    36721 tggttcaaat aattcacttt caaatgaatg cgtcagtggt ggcaaacgca tcaggatcaa
    36781 agtgaacatc acgaaacttc ttacaatggc gcatcacctt cggcataaaa ggaaaagaag
    36841 atgcccactt ctcatgaaaa tgcactgcaa caacgttgcc agcaaattgt caccagccca
    36901 gtgcttagcc cggagcagaa gcgccatttt ctggcactgg aagcagaaaa caatctgcct
    36961 tatccgcagc ttcctgccga agcccgccgc gcgctggatg agggtgtaat ctgcgatatg
    37021 tttgaaggtc atgcgccgta caaaccgcgc tatgtcttac ccgattacgc ccgttttctg
    37081 gcgaacggtt ccgaatggct ggagctggaa ggcgcgaaag atcttgatga cgcactctct
    37141 ctgctgacca ttctttacca ccacgtaccg tcggtcacat cgatgccggt ctacctgggg
    37201 caactggatg cgttgttgca accatatgtt agaattctaa cacaagacga gatcgatgtt
    37261 cgaataaaac gtttctggcg ttacctcgac agaaccctgc cagacgcctt tatgcacgcc
    37321 aatatcggcc cgtctgattc gcccattacc cgcgcgatct tacgtgcaga cgcagagttg
    37381 aagcaggttt caccgaacct gacctttatc tacgatcctg aaatcacccc tgatgacctg
    37441 ctgctggaag tggcgaagaa catctgtgaa tgtagcaaac cgcacatcgc caacggtccg
    37501 gtgcatgata aaattttcac aaaagggggc tacgggattg tgagctgtta caactcactg
    37561 ccgctggcgg gtggtggcag cacgctggta cgccttaacc tgaaagccat tgccgagcgc
    37621 agcgaatcgc tggatgactt ctttacgcgc actctaccgc actactgcca gcagcagatc
    37681 gccatcatcg atgcgcggtg tgaattcctc tatcaacaat cacacttctt tgagaatagc
    37741 ttcctggtga aagaagggct gattaaccct gaacgttttg tgccaatgtt tggcatgtat
    37801 gggctggcgg aagcggttaa cttgctgtgt aagaaagaag ggattgccgc gcgctacggt
    37861 aaagaagccg ccgcaaatga agtgggttat cgcatcagcg cgcaactggc ggagtttgtc
    37921 gccaataccc ccgtgaaata tggctggcaa aaacgcgcca tgttacacgc acagtcgggg
    37981 atcagttccg atatcggcac cacgccgggc gcgcgtttgc cgtatggtga tgagccagat
    38041 ccgatcaccc atctgcaaac tgtcgccccg catcatgctt attattattc cggcatcagc
    38101 gacattctga cgctcgacga aaccatcaaa cgtaatccgc aggcgctggt acagctttgc
    38161 ctcggtgcct ttaaagccgg aatgcgtgaa tttactgcca atgtcagcgg taacgatctg
    38221 gttcgcgtta ccggttatat ggtgcgtttg tcggatttag aaaaatatcg cgccgaaggt
    38281 tcacgcacca acaccacctg gctgggcgaa gaagccgcac gcaacactcg tattctggaa
    38341 cgccagccgc gcgtgataag ccatgaacag cagatgcgct ttagtcagta agattatccc
    38401 cttctcctgc gttgacgggc caggcagtcg tctggctctg tttttgcagg gctgcaatct
    38461 gcgctgcaaa aactgtcaca atccgtggac gatgggacgt tgcaatgact gtggggagtg
    38521 cgtgccacag tgtccgcatc aggcgttgca gattgttgac ggcaaagtgg tgtggaacgc
    38581 tgtggtttgc gagcagtgtg atacctgcct gaagaggtgt ccgcaacatg ccacgccaat
    38641 ggcgcaatcc atgagcgtgg acgaagtgct tagccatgtc cgcaaagcgg tgctgtttat
    38701 tgaagggata acggtaagcg gcggcgaagc cacgacccaa ctgccgtttg tagtggcgct
    38761 gtttactgct atcaaaaacg atccgcaact gcgccacctc acctgcctgg tggacagtaa
    38821 cggcatgttg agcgaaaccg gctgggaaaa attactcccg gtgtgtgacg gcgcaatgct
    38881 cgatctcaaa gcgtggggga gcgaatgtca tcaacactta accggacgcg ataatcagca
    38941 gattaagcgc agcatctgtt tgcttgcaga gcgcggcaag ctggcggaac tgcgtttgct
    39001 ggtaattcca gaccaggtgg attatttgca tcacatcgat gagctggcga cgtttatcaa
    39061 gagacttggc gatgttccgg ttcgcctgaa tgcgtttcat gcccacggcg tgtatggcga
    39121 ggcgcaaagc tgggcgagcg ccacgccgga agacgttgag ccgttggctg atgcgttaaa
    39181 ggtgcgcggg gtgagccggt tgatatttcc ggcgctctat ttgtgacatg ccggatacgg
    39241 cctacggttc ggcacagact tgtaggcatg ataagacgcg acaagcgtcg catcaggcat
    39301 tatgcgccga ctgccggatg cggcgtgaac gccttatccg gcctacggtt cggcacagac
    39361 ttgtaggcat gataagacgc gtcagcgtcg catcaggcat tgtgcgccaa ctgccggatg
    39421 cggcgtgaac gccttatccg gcctacggtt cggcacagac
//
LOCUS       DAZPDU010000018        39409 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZPDU010000018 DAZPDU010000000
VERSION     DAZPDU010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39409
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.018"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..266
                     /locus_tag="ACHURS_000856"
     CDS             <1..266
                     /locus_tag="ACHURS_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFR1236658.1"
                     /translation="TKAEAPAAQSKAAVPAKASDEEGTRVSINNASAEELARAMNGVG
                     LKKAQAIVSYREEYGPFKTVEDLKQVPGMGNSLVERNLAVLTL"
     gene            360..758
                     /gene="fadM"
                     /locus_tag="ACHURS_000857"
     CDS             360..758
                     /gene="fadM"
                     /locus_tag="ACHURS_000857"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFR1236659.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(810..1505)
                     /gene="queC"
                     /locus_tag="ACHURS_000858"
     CDS             complement(810..1505)
                     /gene="queC"
                     /locus_tag="ACHURS_000858"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFR1236660.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(1570..3270)
                     /locus_tag="ACHURS_000859"
     CDS             complement(1570..3270)
                     /locus_tag="ACHURS_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFR1236661.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHRETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            3370..4188
                     /gene="cof"
                     /locus_tag="ACHURS_000860"
     CDS             3370..4188
                     /gene="cof"
                     /locus_tag="ACHURS_000860"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFR1236662.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            4340..4798
                     /gene="decR"
                     /locus_tag="ACHURS_000861"
                     /pseudo
     CDS             4340..4798
                     /gene="decR"
                     /locus_tag="ACHURS_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
     gene            4828..6600
                     /locus_tag="ACHURS_000862"
     CDS             4828..6600
                     /locus_tag="ACHURS_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFR1236663.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDCVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVSEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLAQQSGWYRD
                     MYRYQQLEAALDDAPENHEEAVDA"
     gene            6593..8374
                     /locus_tag="ACHURS_000863"
     CDS             6593..8374
                     /locus_tag="ACHURS_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFR1236664.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSSNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            8555..8893
                     /gene="glnK"
                     /locus_tag="ACHURS_000864"
     CDS             8555..8893
                     /gene="glnK"
                     /locus_tag="ACHURS_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFR1236665.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            8923..10209
                     /gene="amtB"
                     /locus_tag="ACHURS_000865"
     CDS             8923..10209
                     /gene="amtB"
                     /locus_tag="ACHURS_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFR1236666.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(10258..11118)
                     /gene="tesB"
                     /locus_tag="ACHURS_000866"
     CDS             complement(10258..11118)
                     /gene="tesB"
                     /locus_tag="ACHURS_000866"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFR1236667.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            11336..11908
                     /locus_tag="ACHURS_000867"
     CDS             11336..11908
                     /locus_tag="ACHURS_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFR1236668.1"
                     /translation="MKLVHMASCLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPMQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(11939..12250)
                     /locus_tag="ACHURS_000868"
     CDS             complement(11939..12250)
                     /locus_tag="ACHURS_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFR1236669.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            12413..12509
                     /gene="ffs"
                     /locus_tag="ACHURS_000869"
     ncRNA           12413..12509
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHURS_000869"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            12629..12982
                     /locus_tag="ACHURS_000870"
     CDS             12629..12982
                     /locus_tag="ACHURS_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 family protein"
                     /protein_id="HFR1236670.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     NVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(13024..14572)
                     /gene="pdeB"
                     /locus_tag="ACHURS_000871"
                     /pseudo
     CDS             complement(13024..14572)
                     /gene="pdeB"
                     /locus_tag="ACHURS_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
     gene            complement(14736..15206)
                     /locus_tag="ACHURS_000872"
     CDS             complement(14736..15206)
                     /locus_tag="ACHURS_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFR1236671.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(15322..15873)
                     /gene="maa"
                     /locus_tag="ACHURS_000873"
     CDS             complement(15322..15873)
                     /gene="maa"
                     /locus_tag="ACHURS_000873"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFR1236672.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(16044..16262)
                     /locus_tag="ACHURS_000874"
     CDS             complement(16044..16262)
                     /locus_tag="ACHURS_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFR1236673.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(16288..16662)
                     /gene="tomB"
                     /locus_tag="ACHURS_000875"
     CDS             complement(16288..16662)
                     /gene="tomB"
                     /locus_tag="ACHURS_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFR1236674.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(17208..20357)
                     /gene="acrB"
                     /locus_tag="ACHURS_000876"
     CDS             complement(17208..20357)
                     /gene="acrB"
                     /locus_tag="ACHURS_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFR1236675.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTSLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(20380..21573)
                     /gene="acrA"
                     /locus_tag="ACHURS_000877"
     CDS             complement(20380..21573)
                     /gene="acrA"
                     /locus_tag="ACHURS_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFR1236676.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYLDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            21715..22362
                     /gene="acrR"
                     /locus_tag="ACHURS_000878"
     CDS             21715..22362
                     /gene="acrR"
                     /locus_tag="ACHURS_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFR1236677.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            22490..25852
                     /gene="mscK"
                     /locus_tag="ACHURS_000879"
     CDS             22490..25852
                     /gene="mscK"
                     /locus_tag="ACHURS_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFR1236678.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            25913..26011
                     /locus_tag="ACHURS_000880"
                     /pseudo
     CDS             25913..26011
                     /locus_tag="ACHURS_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(26064..26225)
                     /gene="rsmS"
                     /locus_tag="ACHURS_000881"
     CDS             complement(26064..26225)
                     /gene="rsmS"
                     /locus_tag="ACHURS_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFR1236679.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(26239..26766)
                     /gene="priC"
                     /locus_tag="ACHURS_000882"
     CDS             complement(26239..26766)
                     /gene="priC"
                     /locus_tag="ACHURS_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFR1236680.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALCHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            26836..27213
                     /locus_tag="ACHURS_000883"
     CDS             26836..27213
                     /locus_tag="ACHURS_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFR1236681.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            27366..27917
                     /gene="apt"
                     /locus_tag="ACHURS_000884"
     CDS             27366..27917
                     /gene="apt"
                     /locus_tag="ACHURS_000884"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFR1236682.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            28046..29971
                     /gene="dnaX"
                     /locus_tag="ACHURS_000885"
     CDS             28046..29971
                     /gene="dnaX"
                     /locus_tag="ACHURS_000885"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFR1236683.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMPAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVVPTAVMTPTQVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQCVQGATKAKKSESAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKASAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     AKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    29304..29368
                     /gene="dnaX"
                     /locus_tag="ACHURS_000885"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            30024..30353
                     /locus_tag="ACHURS_000886"
     CDS             30024..30353
                     /locus_tag="ACHURS_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFR1236684.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            30353..30958
                     /gene="recR"
                     /locus_tag="ACHURS_000887"
     CDS             30353..30958
                     /gene="recR"
                     /locus_tag="ACHURS_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFR1236685.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            31068..32942
                     /gene="htpG"
                     /locus_tag="ACHURS_000888"
     CDS             31068..32942
                     /gene="htpG"
                     /locus_tag="ACHURS_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFR1236686.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            33123..33767
                     /gene="adk"
                     /locus_tag="ACHURS_000889"
     CDS             33123..33767
                     /gene="adk"
                     /locus_tag="ACHURS_000889"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFR1236687.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYFKEAEAGNTKYAKVDGTKSVAEVRADLEKILG"
     gene            33899..34861
                     /gene="hemH"
                     /locus_tag="ACHURS_000890"
     CDS             33899..34861
                     /gene="hemH"
                     /locus_tag="ACHURS_000890"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFR1236688.1"
                     /translation="MRQTKIGILLANLGTPDTPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(34858..35817)
                     /gene="aes"
                     /locus_tag="ACHURS_000891"
     CDS             complement(34858..35817)
                     /gene="aes"
                     /locus_tag="ACHURS_000891"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFR1236689.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            35969..37273
                     /gene="gsk"
                     /locus_tag="ACHURS_000892"
     CDS             35969..37273
                     /gene="gsk"
                     /locus_tag="ACHURS_000892"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFR1236690.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(37406..39082)
                     /gene="ybaL"
                     /locus_tag="ACHURS_000893"
     CDS             complement(37406..39082)
                     /gene="ybaL"
                     /locus_tag="ACHURS_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFR1236691.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(39320..>39409)
                     /locus_tag="ACHURS_000894"
     CDS             complement(39320..>39409)
                     /locus_tag="ACHURS_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1236692.1"
                     /translation="SIELVYKICAFLPLLGMLTIFLPDNRHKD"
BASE COUNT         9528 a        10050 c        10417 g         9414 t
ORIGIN      
        1 aaaccaaagc ggaagctcct gcagcacaaa gtaaagcagc agtaccggcg aaagccagtg
       61 acgaagaagg cacccgggtc agcattaata atgccagcgc ggaagagcta gcccgcgcga
      121 tgaatggcgt tggcctgaag aaagcgcagg cgattgtcag ttatcgcgaa gagtacggtc
      181 cgtttaaaac ggtggaggat ctaaagcagg tgccggggat gggcaattcg ctggtggaac
      241 gtaatctggc ggtattaacc ctgtaattaa tttgcatagt ggcaattttt tgccagactg
      301 aagaggtcat accagttatg acctctgtac ttataacaac aacgtaaggt tattgcgcta
      361 tgcaaacaca aatcaaagtt cgtggatatc atctcgacgt ttaccagcac gtcaacaacg
      421 cccgctacct tgaatttctc gaagaagccc gctgggatgg gttggaaaat agcgacagtt
      481 ttcagtggat gacggcccat aacatcgcct tcgtcgtggt caatatcaat attaactatc
      541 gtcgcccagc ggtattaagc gacctgttaa ctattaccag tcagttgcag caattaaacg
      601 gtaaaagcgg catcttaagc caggtcatta cactggagcc ggaagggcag gtggtagcgg
      661 atgcgcttat tacgtttgtt tgtattgatc ttaaaacgca gaaagcatta gctctggaag
      721 gggaattgcg cgaaaagctg gagcagatgg ttaagtaaac gttttgtggt gccggatgct
      781 caagccgcat ccggcgacac ccggaataat tacctcaacc cggttttctg cttcatcgct
      841 gccatcaccg tcggtttatc ggccagataa tgattcaaac cgttggcgcg taaattacat
      901 gccgcacaat gaccgcaacc gtcgccttta atgccgttat agcaggtcaa cgtttcgtta
      961 cggactaaat ccagtttgcc gtaataatct gccagcgccc aggtttccgc tttatcaatc
     1021 cacatcagcg gcgtttcaaa acgaatatct ttcgccatgc ccaaactgac ggcatggttt
     1081 agtgctttca caaactcatc gcggcaatcc gggtagccgg agaaatccgt ttcacagacg
     1141 ccggtaatta cggcttctgc ttttacctga tacgcatata ttgccgccag cgtcaggaac
     1201 aaaatattac gcccagggac aaacgtattc gggatgccat cggcttcagg ttcataatca
     1261 ggcaccggaa tgctgtcacg tgtcaggctg ctgaccgcca gctcgttgag cagcgttaca
     1321 tcaagaacct tatgcgcgcg tgctcccagt ttcagcgcca gttcgcgtgc cacgtcgatt
     1381 tctgcgcgat gtcgctgacc gtaatcgaac gtcacgcaat ggacttcatc atattgttgt
     1441 aatgcctgca ccagacaggt ggtggaatcc tggcctccac tgaacacaac gacagcacgt
     1501 ttcatagata atcctgcttg aacaataaaa gcgttatggt aacgcctgcg attaacccgg
     1561 accagctatt cattgcgacg gcgcgggaag ccaggcttcg gtaaattcaa accagccgcg
     1621 cggtgtcagt cgcacaccgt tcacgccggg aggggcacta atgcgatagt gatagttgaa
     1681 cagcggcgtc agcgtcgcgt ctgccattaa ctggctaaaa accgctttca gggcattaaa
     1741 tcggttttcc tcatcaggca ttacttgcac cgcgtccagc gtcgattgca gatgtgcata
     1801 tgctggagcg tcgaaaacat gtggccatag tggatcgcaa cgtagccatt gctccagagt
     1861 atattccggt gcttcgccaa ttaatctgtc gcccatcatg aggtctgcgt gtgccagtag
     1921 ggtcgtgtcg tcccagtttt ttgcgttatg aaaaataatt gtgagttcac agccttccgc
     1981 tgccagtgtc gcctgtaggc gttctgccat ggtatgaagt tctatcggta ggtgataaac
     2041 cagcgtcaag gttttcggta gtttgacttc atccggtact tgccagtgcg gaatagtcca
     2101 gcctggcagt aatgcatgac tggcggtgat caggttttct ccgacttcta acgtttgtaa
     2161 taaaccggat tgatgaataa tggagatcac ttttcgcgcc tgccagaggg agagtcgggg
     2221 acttttgcgc aacgttaaat agcaaaaacc taaactgatg ccactactga cctggctgac
     2281 ccgttgcagc tcctccggtt tgccgatggt gatttgcacg ggatgccgac aactggttcc
     2341 caaatctttt tcgaaaagcg gcggagttat ccagtactca accgctttaa gcagcggatg
     2401 acgcaaatgg taataatcat ggctctccag gcgcaccagc tccgctgtga attgtgttaa
     2461 gcgaaaagga ccagtgccga tcagtgggaa ttgcggatgc gccagatggc tgcaatagct
     2521 cgccagccgg tgcgcaagcc agtaatcggg gcgatgtaaa aagaaggtca gacactgcgg
     2581 atgggtgact tcaatacgct tcacgctaat aaataattga tccagtgctg gcagttgtaa
     2641 cagcatcaat aatcgctggt gtaagtgtga ggcttttact gcatcgccgt tatgccagtg
     2701 tagggttgaa cgaagataaa agtcccagcg taacccgtca gtagaggttt ccctgtgatg
     2761 cgctaaatcg ccaatcgggc gctgagtatt attatcgaag cgggtcaggc cggaaaatat
     2821 ctgcccggcg agatgctgct cggcacggcc gggcaaaaag cctggttgta gcggttcgag
     2881 cgggcgatag tagggaatac gcaacgtggg tgtatcgttt tgccattgtc cgcccataaa
     2941 cggctgtaac agagtgcgca gctcacctgg ggccagttgc gccagctcca gcacatcttg
     3001 ctgctttccg gtttccagtg cctgttccat catcgcattg cgtagcgatt ccggcgtgac
     3061 cagaaagcgt aattgtccgc gctttccgcg tcctgactgc gcctgccact ccagccatcc
     3121 cgcctcctgt gcctgacgca acagcgtacg aacatggcgt tcgctgcaaa aacagcgttc
     3181 ggccagttcg ctgacggtga cggtttgcgg ctttccggct gaaggttgcc acagacgttg
     3241 atactggtta agacggttga gcaatcgcat ataaacccgg aacaatatta tttaactatt
     3301 cactattact tccgtatata tcaggtgata ctcaatctcc attaaccgtg tcacagagtg
     3361 gagaaagaaa tggctcgtct ggcagcattt gatatggatg gcactttatt gatgcctgac
     3421 catcatttag gtgagaaaac cctctctact ttggcgcgac tgcgtgaacg cgacattacc
     3481 ctcacttttg ccacggggcg tcatgcgctg gagatgcagc atattctcgg ggcgctatcg
     3541 ctggatgcat atttgattac cggcaacgga acgcgcgtgc attctctgga aggtgaactt
     3601 ttacatcgtg atgatttacc tgcggatgtc gcggagctgg tgctgtatca gcaatgggat
     3661 acccgagcca gcatgcatat cttcaatgac gacggttggt ttaccgggaa agagatccct
     3721 gcgttgttgc aggcatttgt ctatagcggt tttcgttatc agataatcga tgtcaaaaaa
     3781 atgccactcg gcagcgtcac caagatctgc ttctgtggcg atcacgacga tcttacacgc
     3841 ttgcagatcc agctatacga agcattaggc gagcgtgcac atttgtgttt ttccgccaca
     3901 gattgcctcg aagtgctgcc ggtgggctgc aataaaggcg ctgcattgac ggtgctgacc
     3961 caacatttag gtttatcgtt gcgcgattgc atggcctttg gtgatgcgat gaacgatcgc
     4021 gaaatgttag gcagcgtcgg tagcgggttt attatgggca atgcgatgcc gcaactgcgc
     4081 gcggagctcc cgcatttacc ggtgattgga cattgccgaa atcaggctgt ctctcactat
     4141 ttgacgcact ggctggacta tccacatcta ccttattccc ccgaataacg agatcccttc
     4201 cagcaccggg caattgcccg gtttttttgc gttgaatttg tcattttgtg ccgtggtgtt
     4261 taaaccgcac agaataaatt gtcgtgattt cacctttaaa atagaattaa aagagaaaaa
     4321 aattctccgt ggaagggcta tgttagataa aattgaccgt aagctgctgg ccttactgca
     4381 gcaggattgc accctctctt tgtaggcact ggctgaagcc gttaatctga caaccacccc
     4441 ttgctggaag cgcctgaaac ggctggagga cgacggtatc cttatcggca aagtcgccct
     4501 gctggatccg gaaaaaatag gcctcggcct gaccgccttt gtgctgataa aaacgcaaca
     4561 tcacagcagc gaatggtatt gccgctttgt cacggtggtt accgaaatgc cagaagtgct
     4621 ggggttctgg cgcatggctg gtgaatacga ttatctgatg cgcgtccagg ttgccgacat
     4681 gaaacgctac gacgagtttt ataagcgtct ggtaaacagc gtgccggggc tgtcggacgt
     4741 cacttccagc ttcgcgatgg aacagattaa atacaccact tctttaccca tcgaataaat
     4801 atccagaatc aggtcaggac acaacgcgtg cgattatttg ctcaattaag ctggtatttc
     4861 cgtcgggaat ggcgtcgcta tctcggggct gtcgccttgc ttgtcattat cgcgatgctg
     4921 caactggttc cgccaaaagt ggttggtatt gttgtcgatg gcgtgacaga acaacacttt
     4981 actaccgggc tgatcctgat gtggatcgcc accatggtgc tgattgccgt tgtggtttat
     5041 ctcctgcgtt atgtctggcg cgtattgctg tttggcgcgt cttaccaact ggctgttgaa
     5101 ctgcgtgaag attattaccg tcagctaagc cggcagcacc cggagtttta cctgcgtcat
     5161 cgcacgggtg atctcatggc tcgtgcgacc aatgacgtcg attgcgtcgt gtttgccgcc
     5221 ggagaagggg tgctgacgct ggtggattca ctggtaatgg gctgcgctgt gttgattatg
     5281 atgtctacgc aaattagctg gcagttgacc ttatttgccc tgttgccgat gccagtgatg
     5341 gcgatcatga ttaagcgcaa cggcgatgct ttgcatgaac gctttaagct ggcacaggcg
     5401 gcgttttcca gtcttaatga ccgcacccag gaaagcctca ccagtatccg catgatcaaa
     5461 gcctttggtc tggaagatcg ccagtcggcg ttatttgccg cggatgccga agataccggc
     5521 aaaaaaaaca tgcgggtggc gcgtattgat gctcgtttcg acccgaccat ctatatcgcg
     5581 attggtatgg cgaacttgct ggcgattggc ggcggtagtt ggatggtggt gcagggcagt
     5641 ttaacgctgg gccagctcac cagttttatg atgtatttag gtctgatgat ttggccaatg
     5701 ctggcgctgg catggatgtt taacattgtg gaacgtggta gtgctgcgta cagccgtatt
     5761 cgtgcgatgc tggcggaagc gccggtggtg aacgatggta gcgaacccgt gtcggaaggg
     5821 cgtggcgaac ttgatgtaaa tattcaccag ttcacgtatc cgcagactga ccatcctgcg
     5881 ctggaaaacg tcaatttcgc cctgaaaccc ggtcagatgc tgggtatctg cgggccgact
     5941 ggttccggca aaagtaccct gttgtcgctc attcagcgtc atttcgacgt tagcgagggg
     6001 gatattcgct ttcatgatat tcctctgacg aagttacaac tcgatagctg gcgtagccgc
     6061 ctggcggtgg tcagccagac gccattcctt ttttctgaca ccgtggcaaa taacatcgcg
     6121 ctgggttgcc cgaatgccac ccagcaagag attgagcatg tcgcgcggtt agccagcgta
     6181 catgacgata ttttgcgttt accgcaaggt tacgatacag aggtgggcga gcgcggcgtg
     6241 atgctttccg gcgggcaaaa acagcgtatc tctattgctc gtgcgttatt agtcaacgcg
     6301 gaaatcctca tccttgatga tgcgctttcg gcggtggacg gacgcacaga gcaccagatc
     6361 ctgcataacc tgcgtcagtg gggacaggga agaacggtaa tcatcagtgc ccatcgcctt
     6421 tcggcactga cggaagccag tgaaattatt gtgatgcagc acggacatat cgcccagcgt
     6481 ggcaatcatg atgtgctggc gcaacaaagc ggctggtatc gcgatatgta tcgctatcaa
     6541 caactggagg cggcgctcga cgacgctccg gaaaatcacg aggaggccgt cgatgcgtag
     6601 ttttagccaa ctgtggccga ctctcaagcg cctgttagcg tacggttcac cgtggcgtaa
     6661 accgctgggg attgcggtcc tgatgatgtg ggttgcggcg gcggcagaag tcagtgggcc
     6721 gctgcttatc agctatttta tcgacaatat ggtagcgaaa aataacctgc cgttgaaagt
     6781 ggttgcaggg ctggctgcgg cgtatgttgg gctacaactg tttgccgccg ggctacatta
     6841 cgcgcagtcg ctgctgttta atcgggcggc agtaggcgta gtgcaacagt tgcgtaccga
     6901 cgtgatggat gctgcgttac gccagccatt aagcgagttt gatacccaac ccgtcgggca
     6961 ggtgatttcc cgcgtcacta atgacactga agtgatccgc gatctctacg ttaccgtagt
     7021 ggcaactgtc ctgcgcagtg ccgcactggt gggcgcgatg ctggtggcga tgttcagcct
     7081 cgactggcga atggcgctgg tggcgataat gattttcccg gtagtgctgg tggtaatggt
     7141 gatatatcaa cgttacagca cgccgattgt ccgtcgtgtg cgcgcctatc tggcggatat
     7201 caacgacggc tttaacgaaa tcatcaatgg catgagcgtc atccagcagt tccgtcagca
     7261 ggcgcgattt ggcgaacgta tgggtgaggc cagtcgttca cactatatgg cgaggatgca
     7321 aaccctgcgc ctcgacggtt ttctgctgcg tccgctgctg agtctgtttt catcgctcat
     7381 tctttgtgga ttgttgatgc tgtttggctt ctccgccagc ggcaccattg aagtgggcgt
     7441 gctgtatgcc tttatcagct atcttgggcg acttaacgaa cccttaatcg aactgaccac
     7501 gcaacaggcg atgctgcaac aggctgttgt tgctggtgag cgcgtgtttg aactgatgga
     7561 cggaccgcgc cagcaatatg gcaatgatga ttacccgtta cagagtggca ccatcgaagt
     7621 cgataacgtg tcatttgctt atcgcgatga caatctggtg ctaaagaaca ttaatctctc
     7681 tgtgccttcg agtaactttg tggcgctggt cgggcatacc ggcagtggta aaagtaccct
     7741 cgccagttta ttgatgggct attacccgct aacggaaggt gagattcgcc ttgatggtcg
     7801 tccattaagt tcgctaagtc acagcgcgct gcgccagggc gtggcaatgg tgcagcaaga
     7861 tccggtggtg ctggcggata ccttcctcgc caacgtgacg ctggggcggg atatctccga
     7921 agaacgcgtc tggcaggcgc tggaaaccgt gcaactggcg gagctggcgc gtagcatgag
     7981 cgacggtatt tacacgccgc tgggcgagca ggggaataat ctctcagttg ggcaaaagca
     8041 actgctggca ctggcgcgcg tgctggtcga gacgccgcaa atcctgatcc ttgatgaggc
     8101 aaccgccagc attgactccg ggactgaaca ggcgattcaa catgctctgg cggcggtgcg
     8161 tgaacatacc acgctggtgg tgattgctca ccgcttatcg accattgttg atgccgacac
     8221 cattctggtg cttcatcgtg ggcaagccgt ggagcagggc actcaccagc aactgctggc
     8281 tgcccaggga cgctactggc agatgtatca actgcaactt gcgggcgaag agctggcagc
     8341 cagcgtgcgt gaagaggaat cattgagcgc ctgaatagcg caatatttca tcgttggtgc
     8401 aaaaatgtaa cgcactgtgc actgtcatag tgcgttttca ttttcaaact tcttaacttc
     8461 ctgctctctt tctcgttttt catttctggc acaccgcttg caataccttc ttcgtgtagc
     8521 agaaccatta ccgaattctg accggagggg atctatgaag ctggtgaccg tgataatcaa
     8581 accattcaag ctggaagacg ttcgtgaagc gttatcttcc attggtattc agggcctgac
     8641 cgtcaccgaa gtgaaaggtt tcgggcgtca gaaagggcat gccgagctgt accggggggc
     8701 ggaatacagc gtcaacttcc tgccaaaagt aaaaattgat gtggcgattg ctgatgacca
     8761 actcgatgaa gtgatcgata tcgtcagtaa ggcggcttac accggaaaaa ttggcgacgg
     8821 caaaatcttc gtcgctgaat tgcaacgcgt cattcgtatt cgtaccggcg aagccgacga
     8881 agcggcgctg taatctctgg cacacagcaa caggaacgaa aaatgaagat agcgacgata
     8941 aaaactgggc ttgcttcact ggcgatgctg ccgggactgg taatggctgc acctgcggtg
     9001 gccgataaag ccgacaatgc gtttatgatg atttgtactg cgctggtgct gtttatgact
     9061 attccgggga ttgccctgtt ttacggtggg ttgattcgcg gcaaaaacgt gctgtcgatg
     9121 ctgacgcagg tgacggtgac gtttgcactg gtctgtattc tctgggtggt ttacggttac
     9181 tcgctggcgt ttggtgaggg caacaacttc ttcggcaaca ttaactggtt gatgctaaaa
     9241 aacatcgaac tgacggcggt gatgggcagc atttatcagt atatccacgt ggcgtttcag
     9301 ggctcgtttg cctgcattac cgtcggcttg atcgttgggg cgctggcgga acgaatccgc
     9361 ttctcagctg tgttgatttt cgtggtggta tggctgacgc tctcttacat tccgattgcg
     9421 catatggtgt ggggcggtgg tttgctggct tctcacggtg cgctggattt cgcgggtggc
     9481 acggtggtgc atattaacgc cgcaatcgcc ggtctggtgg gcgcgtatct gataggcaaa
     9541 cgcgtgggct tcggcaaaga ggcgtttaaa ccgcacaacc tgccaatggt cttcaccggt
     9601 actgccattc tctatattgg ttggtttggc tttaacgccg ggtcagcggg cacggcgaat
     9661 gaaatcgcgg cactggcatt tgtgaatact gtggtcgcaa cggcggcggc aattcttggc
     9721 tggatcttcg gtgaatgggc gctgcgtggt aagccttctc tgctgggagc gtgttctggc
     9781 gcgattgccg gtctggtcgg cgtgacgcca gcgtgtggct acattggggt tggcggcgcg
     9841 ttgattatcg gcgtggtagc tggtctggcg ggcttgtggg gcgttactat gctcaaacgc
     9901 ttgctgcggg tggatgatcc ctgcgatgtc ttcggtgtgc acggcgtttg tggcattgtc
     9961 ggctgtatca tgactgggat ttttgccgcc agttcgctgg gtggcgtggg cttcgctgaa
    10021 ggtgtgacga tgggccatca gttgctggta cagctggaaa gcatcgccat tacgatcgtc
    10081 tggtccggtg tggtggcatt tattggctac aaactggcgg atctgacggt tggtctgcgt
    10141 gtgccggaag aacaggagcg agaagggctg gacgtcaaca gccacggcga gaatgcctat
    10201 aacgcgtaac aagcactgca aaaaacagcc ggacggtttt cacctccggc tattttttta
    10261 attgtgatta cgcatcaccc cttcctgaac ggtcgaggca accagcacgc cgtcctgagt
    10321 ataaaactca ccgcgcacaa agccgcgtgc gctggacgcc gaggtgctct ccacgctata
    10381 cagcagccat tcattcaaat taaacgggcg atgaaaccac atggaatggt caatggtggc
    10441 aatctgaatc cccggttcga gaaaaccgat gccgtgcggc tgtagagcta ccggcaggaa
    10501 gttaagatca gaagcgtaac cgagcagata ctgatgaacg cgcaggtcat ccggcacgct
    10561 accatttgcg cggatccaca cctgacgatg tggttctgcg acgtgacctt tcagtgggtt
    10621 atgaaactcc accggacgga cttccagcgg acgatcgcag atgaatttat ctttcagcac
    10681 tggcggcagc aggtgcgcca gcgattgggc gatttgtgtt tccgaaggca ggccatcagg
    10741 cgctggcgcg gacggcattg ttttttgatg ttcgaaaccc gcttcaggtg cctggaaaga
    10801 ggccgtcata taaaaaatcg gtttgccgtt ttgaatagca gcaacccggc gggcgctgaa
    10861 gctgttaccg tcacgcagcg tttcgacatc ataaataatc ggcttcttac tatcgccagg
    10921 gcgaagaaag tagctgtgaa acgaatgtac cagccgctct tcagggacgg tctcttttgc
    10981 agcatacaag gcctgaccca cgacctggcc gccaaacacc tggcgtaaac ctaaatcttc
    11041 actctggccg cgaaagagtc cttcctcaat tttttccaga tttaacaatg tcagtaaatt
    11101 ttttagcgcc tgactcatat aactctccag taactaagct gccgcagcaa gccaaagtga
    11161 gttgagtata acgcaaattt gccactggtc cgatgggtgc aatagtctga attacgggct
    11221 aattacaggc agaaatgcgt gatgtgtgcc acacttgttg acgttactat tttgttaacc
    11281 actcttccgg cgaggaaagt tagcccgctg gtgcattgat aataaggaga agtgaatgaa
    11341 actcgtgcac atggccagtt gtttagcggt tgcgattgcg ttggcggctt gcgcagataa
    11401 aagcgcggat attcagacgc cagcaccggc ggcaaatacg tctatttcag caacacaaca
    11461 acctgctatc cagcaaccga atgtctccgg taccgtttgg atccgtcaga aagtcgcact
    11521 gccgcctgat gctgtgctga ccgtgacact ttctgacgcg tcgttagccg atgcgccgtc
    11581 aaaagtgctg gcgcagaaag cggtgcgtac cgaaggtaaa cagtcaccat tcagctttgt
    11641 tctgcctttt aacccggcag atgttcagcc gaacgcgcgt attctgttga gtgcggcgat
    11701 taccgtgaat gacaaactgg tatttattac cgataccgtt cagccggtga tcaacaaggg
    11761 cggaactaaa gccgacctga cattggtgcc gatgcagcaa accgccgtgc cggttcaggc
    11821 cagcggtggt gcaacgacta ccgtaccttc gacttcacca actcaggtga atccgtcttc
    11881 ggcagttcca gctcctacgc aatattaagc gacgttaacc ctctccgccc ggagagggtt
    11941 agtagttcca gcgataacgc tgcaagtcga tttgcccgct tcccgatacc atcacacctt
    12001 ctgccagtaa tgcctgtcgc tgacgctgta aatccggtcc ggttagcgaa attgtgccgt
    12061 ggcgattaac cacccggtgc cagggtaagg tgctgccttc agggagacgc tttaacacac
    12121 cgcccacctg gcgcgcggcg cggggcgatc ccgccagttt cgccacatca ccgtaagtgg
    12181 tgacatagcc ttcgggaata gcggcgacga tttgccagac gcgttgggga aatgaatctt
    12241 ctttttccat cttttcttcc tgaggtaatt tttcagcata atctggaaaa acgcccgagt
    12301 gaagtcgcat tgcgcaagaa accagcatct ggcacgcgat gggttgcaat tagccggggc
    12361 agcagtgata atgcgcctgc gcgttggttc tcaacgctct caatgggggc tctgttggtt
    12421 ctcccgcaac gctactctgt ttaccaggtc aggtccggaa ggaagcagcc aaggcagatg
    12481 acgcgtgtgc cgggatgtag ctggcagggc ccccacccat ttctgcctcc caccgtttcg
    12541 tcaaaaaatc ccaacatagc taaactttaa ccacaactga cgtcgcaaga attgtctggc
    12601 tgcgcagtac gcttcggagg tatgtctgat gaagtatgtt gatggttttg tggttgccgt
    12661 tcctgccgat aaaaaggatg cctatcggga aatggccgct aaggctgcgc cattgtttaa
    12721 agagtttggc gcgcttcgta ttgtcgaatg ctgggccagc aatgtaccgg atggcaaagt
    12781 gaccgatttt cgtatggcgg tgaaagcgga agagaatgaa gaggttgtct ttagctggat
    12841 tgaataccct tcaaaagagg tccgcgacgc tgctaatcaa aagatgatgt cggacccacg
    12901 gatgaaagag ttcggcgagt ccatgccgtt tgacggcaag cgaatgatct atggcggatt
    12961 cgagtcaatc atcgacgaat aggtagcgtg acgggctgtg cggcgtgccg tcagcccaaa
    13021 aattcacaaa tgttgctcgg cccagcgtaa gaaatcttct ttcggtaatg ccttgctgta
    13081 gagccagccc tgaccgtagt gcacgccatg ctggcgtagc cactcttctt gtttactggt
    13141 ttcgattccc tccgctacca ttttcagttt cagtgttttt gccatttcga tgatatgcgg
    13201 cgtgacattt ttatattcca gcgcatcaac gaaagattta tcgatcttca gaatgtcgac
    13261 atccagatcc tgtaaataac ttaaacttga atatcccgta ccaaaatcat caagataaat
    13321 ttcatggccc gcctcccggt agcgagaaat tatcggggcg ctggttttcg gatcggcaaa
    13381 ctcgcgttca gtgagttcaa gcgcgatctg tctgggatta acctgattga ttgatcattt
    13441 cacgcagcaa ttgcgggatt ttttccgagg tgagcacggt ggattcaaga ttgatcgaaa
    13501 tatgctgctg tggatgctga cgcagccagt cgcccatatc ttcaaaggcg cttcttataa
    13561 tcagtagcgt caatggctca gaaaggcccg tttgctgtgc cagcggaata aaactatctg
    13621 gtgacaacca actaccgtct gtctgcggcc agcgcgccag tgcctcagca ccgacaattt
    13681 tgccattggc taaggagaca atcggctgat agtgcacgca aatatcacga ttttcgatag
    13741 catcctgcag ccgatgatgc ggtgactgga tacggcgcaa aatacgcagc acaaacatcg
    13801 ctgccagcag gccaatcacc aacccgagcg gtaaccaaat aaagacttgc cgatgccagc
    13861 ctttctgcaa cattttcgtt gaagcccagg tgatgatcga aatattcatc tccgggaagg
    13921 gcaggatatc gtaaatgatt ccattatttt cgatatgctc accgggtgtt ttttgtagcc
    13981 tggtaataat tccctgagca atttcatcgc tgctggttat gacaacgtta tgggcattgc
    14041 caataatggc ggcatcaatt tgccatgagc tatagggaat gacatcaata aaggaagcgg
    14101 ggtcgatcat gacgacataa tgtgccgttc ccatggcgac catgtaacgg ataatgccta
    14161 aatcgttatg cgatgttaac cagacacgat aaccatcttt cgaaatttta cctggctcgg
    14221 ggaaggtatc gggcggactt tcatgctcca gagacgaaca ctggggaacg ttgttatcga
    14281 tataaaccac ttcctgaata tagcgataac tgtaagatac ccgacgcatt tccatgagat
    14341 gggcttcgct acaggcagcg ccttgccatc tttccagctc ttgcagcgca tctttccctt
    14401 gtgtcgccac cttattggcc cgaatagcga cgcgggagga ataggtatcc agctcttcaa
    14461 taaacgatgt ttctacctgc tgatgggcca gccagatgct taagccgaca ggcagcaata
    14521 ctgaaagaat cagtactccc gaaataaggc cgaccagatg tcgtgttctc accagcatat
    14581 ccttattaac acagtgtagg gaaagtatat ctgattgtgg aaaaaggctg gcaggagata
    14641 aggaggtaga gctggtattt catgaagtga tgggtggaaa cgatcattgt catttgacca
    14701 agcgcagagc tttacgcctg gtatgtaacc ctaacttaag atgttgattc ggcgcgagcg
    14761 agggtaaaaa tatcgtaaaa agacagttca cctttacgga cgatcatttt ttgcagttgt
    14821 tccttatgaa catccgcgac acgcggagag ttcaagatgg cttcaaccag cgcggcaggt
    14881 acaaagcgcg tgttgtacca ttcgccgtta tagcaaacgc ggaaatccag cacgtcgata
    14941 tcttcatagc ggtagcgtgg gtagacatcg aaaaagaaga aaccattaag caggataaat
    15001 aatacaacca gtagccagac agagcctgac agcgtttcgg actgcaacat caccgccagg
    15061 gtggcaaaaa aagcaacgta cataccgata aacagccccg gatgtttacg gataaaactg
    15121 atactaaagc ggggtttgtt gtcgcgtttt tcgcgggtat tcagagactc aatcgtttcg
    15181 gtcagcaggc gttgtatttc ggtcatcgtt tgccttcgtg gtatctgcaa ctttccaggg
    15241 aacaccctat tttaacgtgg gaatagtaga ggggaagtaa cagatttacc acaattttgc
    15301 ataacagttg cgaaaaaccg attacaattt tttaattatt ctggctggat taccgcccac
    15361 gacaacgttg tccgggacat cttttgtgac aactgcacct gaggctacca cgacgttatc
    15421 accaatggtc acaccagggt taatgaccgc gcgtccgcca atccagacgt tattaccgat
    15481 ggtgacgggt ttccccagtt cagcaccgct attacgtgct acagggtcga tgggatgtgt
    15541 tgccgtgtag atatgaacgc ctggtgccaa catacagtta tcaccgatgc gaatagggca
    15601 gacatcaagc atcacgcaat cgaagttggc gaaaaaatta ttaccgagaa aaatgttata
    15661 gccatagtca cagcgaaacg ttggctcaat ataagcctct gtcacctgac cgaatagatc
    15721 agcgagaatt tgctggcgta atgtgtgctc ttccgccagg gaatgattgt atcggtgaat
    15781 aagctgacga gcgcgcaggc gatcgcgaga taacgtctca tctgccgagc gatacaactc
    15841 accagcaatc atcttttctt tttctgtgct cattactgac ctccggttgc tgaaaatgcg
    15901 gcaacacagt aagacgaaaa gtcagtgaag agaacgttcc cattacatct gtgtgataaa
    15961 gatcacatag ggtatgtctt catggtgaaa agtataaaat tcttaataaa cagccggtta
    16021 tagctccgaa agcgaattat ttattagcga ataaatttcc atactgagga agggatcttg
    16081 tcgtatagtt tattcatggt caattcggcg aggcggtgat ctgcggctga gtaaaatacc
    16141 gccagttcat tatctgataa ttcgtattta tttttctcga taacacgctc cagcgtgtca
    16201 attgtctggc aacgacgtaa acgcattaaa taatcggttt tcgttaaagg tttttcggac
    16261 atacttctac ccatggttgt aataattcta acaagataaa ctcgcaggat tctctttcgt
    16321 cgcattgaca aaacaacgga atagtcgatt acctgacttc cgccatttct gaagatcctg
    16381 catattaata ccataactac tgaacaacat aaaggtgtca tccagatatt cgtcgatctg
    16441 ctcaatgagc ttattgtctt cattatactt aattttgtaa ttaagtgcga aggtcgcaat
    16501 atgctcaatc agttcattca actggaggtt gatcgccgag gttgggtcgt taacccagcc
    16561 atgattgctt tcttcaaggt ttgcaaggca gtcatgatac agggtttcac agagaaactt
    16621 aagctgtgcg atatcatgtc ttttgggtga gtattcatcc ataacgcgtc cccttcttag
    16681 cggttgaact aacggacacc tttcgggatg gaaaaaactt actgacctgg acttgccctc
    16741 tcgtttgtta gcttaactat aagccactct ttgcaggttt tcatcgcatt attacgaaaa
    16801 attacaatta ggaaggaatc gggacaggac tcactttttc atactagcac taacccgcag
    16861 caggtaaaag cagtctgctc gaactgttca taacatttca ggattatcgc cagatgaaat
    16921 tggtgacact aataccagga ttgctctgaa tatgacgtaa taaccgagga atgaataaag
    16981 aattaccgca aataattaag aatagcctct aaatgattat ggattattaa atctattagc
    17041 gctacttaat ataaattaac taacaatcag tattttttat gaattttatc cgtggttaat
    17101 actggttttc gtatgagatc ctgagttggt ggttcaatta ctccttaatg ttcgtaggtt
    17161 atgcataaaa aaggccgctt gcgcggcctt agtgattaca cgttgtatca atgatgatcg
    17221 acagtatggc tgtgctcgat atcttcattc ttgcggctaa agcggcggcg aaccaccaca
    17281 aagaataccg gaacgaagaa gattgccagt accgttgcgg tcaccatccc gcccattaca
    17341 ccggtaccta ctgcgttctg cgcgccggaa ccagcaccag tactgataac cagcggcata
    17401 acgccgagga taaacgccag cgaggtcatc aggatcggac gtaaacgcat ccgcactgca
    17461 tcaagcgtcg cttcaatcag acctttacct tctttatcca tcaagtcttt ggcgaattcg
    17521 acgataagta tcgcgttctt cgccgacaac ccaatggttg tgagcaggcc tacctggaag
    17581 taaacgtcat tggtcaggcc acggaaggtg gcagccagca acgcaccgat aacccccagc
    17641 ggaacgacca gcataacgga gaacggaatc gaccagctct cgtacagcgc cgccagacac
    17701 aggaatacga caatcaacga aatcgcgtac agtgaaggtg cctggttgcc ggagagacgt
    17761 tcctgatagg acatccccgt ccagtcatag ccaacaccgg taggcagttt gctcgccagt
    17821 tgttccatca gctccattgc ttcaccggta cttttacccg gtgccgcctg gcctaagatt
    17881 tccatggatg gcaggccgtt gtaacgttcc agacgcggcg aaccgtactc ccaacgagaa
    17941 gaggagaacg ccgagaatgg caccatctga ccatcagcag cacgaacata ccagtcgccg
    18001 atatcatccg gcagcatacg gtatttcgct tctgacatga cgtaaacttt cttcacacga
    18061 ccgcggtcga taaagtcgtt cacatagcta ccgccccatg cagcgcccag agaggtgtta
    18121 atgtcgttga tagaaacacc cagcgcctgc gctttttcct ggtcgatatc aatcttaaac
    18181 tgcggggtat cttccagacc gtttggacgt acgctggtca gcatatcagg gtgcttcgct
    18241 gcttctgcaa gcagctggtt acgcgcctga gtcagttttt cgtgaccaag gccagcctgg
    18301 tcaatcagct caaagtcaaa gccggttgca gtacccagtt ccacgattgc gggcaggtta
    18361 aaggcgaaaa ccatcgcatc tttgatttgc gagaaagcgc gtgttgcacg catggtaatc
    18421 gcttcaactt tgttttcttc gcccggacga tcggcccagt ccttcaagga aacgaacgca
    18481 ataccggtat tctgaccacg tcccgcaaag ccgaagccgt taacggcgaa caccgactca
    18541 acgttgttct tttctttggt cagatagtaa tgcgttacct cattgagcac tttctgcgta
    18601 cgttcctgcg ttgcacctgc tggcagctga accatggtca taaatacgcc ctggtcctca
    18661 tctggcaaga aggagcttgg cagacgcacg aacagatagg ccatgccgac cacgatgatc
    18721 agatacagca ccaggtaacg ccccgtactg cgcagaatac cgcctacgct gtcggtgtag
    18781 tggtgcgtgc tcttctcgaa catgcggtta aaccagccga agaagccttt tttaccttcc
    18841 ccgtgatcgc ctttggcaat cggtttcagc atggtggcac aaagagccgg agtcaggatc
    18901 aacgctacca gtaccgacag cgccattgct gaaacaatgg taatagagaa ctgacgatag
    18961 atagcaccag tagaaccgcc aaagaaggcc atcggtacga ataccgccga cagtaccatc
    19021 gcgataccga ccagagcgcc ctgaatctgc cccatcgact tacgggtagc ttcttttggc
    19081 ggcaaacctt cttccgccat aacacgctcc acgttttcta ccacaacgat agcgtcatcc
    19141 accaacaagc cgatggcgag caccatcccg aacattgtta gcgtgtttat cgagaagcca
    19201 aaggcggcaa ggacggcaaa ggtcccgagc aataccaccg gtacggcaat ggtcggaatc
    19261 aacgtcgcgc ggaagttctg caggaacaga tacataacca ggaacacgag gatgatcgct
    19321 tcgaccagcg ttttaaccac ttcgtgaata gagattttca cgaacggcgt ggtgtcgtac
    19381 gggtaaacaa ttttcagacc cgacgggaag aacggttcca tcttcgccag ttcagcacgg
    19441 attgccgcag cggtatccag cgcgtttgca ccggtcgcca gcttgatccc cagaccggaa
    19501 gccggttggc cgttaaactc tgcgatgatg tcgtagttct caccacccag ctcaatcttc
    19561 gccacatcac gcagcagtac gcgggaacca tcctgattca ctttcagcag gattttgccg
    19621 aactcttcag tagaggtcag acgcgtctga gcaataatag aggcgttaag ctgttggcct
    19681 ttcaccggcg gcgtaccacc gagctgaccc gccgcaacct gggcgttctg cgctttgatg
    19741 gcggtaatga catcaaccgg cgttagctgg aatttgttca gctcattcgg gttcatccag
    19801 atacgcatcg cgtactgtga accgaacaac tgaacatcac ccacgcccga cgtacggctg
    19861 atggcatctt tcatattcgc cgctacgtag tcagagatat cctcctgcgt catggtgcca
    19921 tcggtgttga taacgccgac aaccatcagg aagctgctgg atgatttctc aacgctcacc
    19981 ccttgctgct gaacttcttg cggcagcaac ggcatcgcca gctgcagttt gttctgcacc
    20041 tgaacctgcg cgatatccgc atcagtacca gactcaaagg tcagggtgat ctgcacggta
    20101 cccgtggagt cactgttaga ggacatgtac atcaggttat cgataccgtt catattctgt
    20161 tcgataacct gtgtcaccgt gtcctgcact gttttcgcat cagcgccggg gtaggaggcg
    20221 gagatcgtta ctgccggcgg tgcaatcgta ggatattgcg ccaccggcag tttgaggatc
    20281 gccagccccc ctgccaacat gatgataatg gcgatcaccc acgcaaaaat cgggcgatcg
    20341 ataaagaaat taggcatgtc ttaacggctc ctgtttaagt taagacttgg actgttcagg
    20401 ctgagcaccg cttgcggctt gctggttatt atcagcggta acttcttgtg cttttacctg
    20461 gacaccagga cgcactttct gcagcccact tattactacg cgatcgcctg ctttcagacc
    20521 ttctgtcacc agccacttat cgccaatagc ctggcttgca acgatcggac gggtttccac
    20581 tttgtcatcc gcgccaacta ccagtacggt ggcatcacca cgcggcgtac gggttacgcc
    20641 ctgttgcggg actaaaatag cgtttggatt aagcccttct tccagacgtg cgcgcacgaa
    20701 catacccggc agcagagtgt gatccgggtt cgggaagata gcgcgtaggg tgatagaccc
    20761 agtggtctga tcaacggtaa cgtcagagaa ttccagcgta ccgtcctgcg ggaacttaat
    20821 gccgtcactg gtgatcagcg acactttggc tttgccgttc tcttgtttca gcgtgccatt
    20881 cgccagttcc tgtttcaggc gcaggaagtc gttgctggac tgggtcacat caaggtagat
    20941 cggatcaagt tgctgcacgg ttgccagcgc agtcgcctga ccgttctgta ccaatgcgcc
    21001 ttccgtcacg ttcgacttac caatgcgacc gctaatcgga gaggtgactt tggtgtaagc
    21061 cagattgatc cgcgcagttt caacggcagc tttcgccgca gttaccgcag cattcgcctg
    21121 ttgcgcatca gccagagcct gatcgtactc ttgcttactg atgtactgag taccgagcag
    21181 tttctgataa cgattcaccg tcaattgcgc gatattggct gcagcctggg ctttcgccag
    21241 atcacctttc gcactgtcgt atgtcgcctg ataggtcgca ggatcaatct gatagagaga
    21301 gacaccagct tcgatgtcgc taccttcttt gaaattacgc ttcaggataa tcccgctaac
    21361 ttgaggacga acttctgcga tccggtaggc actggtgcga cccggaagct cggttgtgat
    21421 ctgcagaggt tcagttttga ctgttactac gccaacggcg ggcatctgct ggccaccttg
    21481 ttgggcctgt ttgtcgtcac atcctgttag ggctaagctg cctgagagca tcagaacgac
    21541 cgccagaggc gtaaaccctc tgtttttgtt catatgtaaa cctcgagtgt ccgatttcaa
    21601 attggtcaat ggtcaaaagt taataaaccc attgctgcgt ttatattatc gtcgtgctat
    21661 ggtacataca ttcacaaatg tatgtaaatc taacgcctgt aaattcacga acatatggca
    21721 cgaaaaacca aacaagaagc gcaagaaacg cgccaacaca tcctcgatgt ggctctacgt
    21781 cttttctcac agcagggggt atcatccacc tcgctgggcg agattgcaaa agcagctggc
    21841 gttacgcgcg gtgcaatcta ctggcatttt aaagacaagt cggatttgtt cagtgagatc
    21901 tgggaactgt cagaatccaa tattggtgaa ctagagcttg agtatcaggc aaaattccct
    21961 ggcgatccac tctcagtatt aagagagata ttaattcatg ttcttgaatc cacggtgaca
    22021 gaagaacgac gtcgattatt gatggagatt atattccaca aatgcgaatt tgtcggagaa
    22081 atggctgttg tgcaacaggc acaacgtaat ctctgtctgg aaagttatga ccgtatagaa
    22141 caaacgttaa aacattgtat tgaagcgaaa atgttgcctg cggatttaat gacgcgtcgg
    22201 gcagcaatta ttatgcgcgg ctatatttcc ggcctgatgg aaaactggct ctttgccccg
    22261 caatcttttg atcttaaaaa agaagcccgc gattacgtcg ccatcttact ggagatgtat
    22321 ctcctgtgcc ccacgcttcg taatcctgcc actaacgaat aaccctgaat ctgactccag
    22381 gatttttcct ggacattttc gtcgttgcta ttctggttca ctgcgtcgtg atattcttgc
    22441 ggtttgactt tttcaggtcg ttcttcaggt tcagaaacct tcattcatca tgactatgtt
    22501 ccagtattac aaacgatcac ggcattttgt tttttcagca tttattgctt ttgtttttgt
    22561 cttgttatgc cagaacacgg cgtttgcgcg ggcgtcatcg aatggtgatc tgccgacaaa
    22621 agcggacctg caggcgcaac ttgactcact aaataaacaa aaagatcttt ctgctcagga
    22681 caaactggtg cagcaggatc tgacagatac attagccacc ctcgataaaa tcgatcgcat
    22741 aaaagaagag acagttcagc tacggcaaaa agtcgctgaa gcgccggaaa aaatgcgcca
    22801 ggcgaccgcg gcgttaacag cacttagcga tgtcgataac gacgaagaaa cgcgcaaaat
    22861 tctgagtacg ctttcgttgc gccagctgga aactcgcgtt gcccaggcgc tggacgattt
    22921 gcaaaacgca caaaacgatc tggcgtctta taacagccag ctggtttcgt tacagacgca
    22981 gcccgaacgc gtgcaaaatg cgatgtataa cgcttcgcag cagctgcaac aaattcgcag
    23041 tcgtctggat gggactgatg tcggcgagac tgccttacgt cccagccaga aagtgttaat
    23101 gcaggctcag caggcgttgc tgaatgcgga gattgaccag cagcgtaaaa gcctggaagg
    23161 gaacaccgtc ttgcaggata ccttgcaaaa gcaacgtgat tacgtgacgg cgaacagcgc
    23221 ccgtctggag caccagttgc agctgttgca agaagcggta aacagcaagc gcctgacttt
    23281 aactgaaaaa acggcgcagg aagccgtctc cccggatgaa gccgcgcgta ttcaggctaa
    23341 tccgctggtg aaacaggaac tggaaattaa ccagcagtta agtcagcgtc tgattaccgc
    23401 gactgaaaac ggtaatcagt tgatgcagca aaacattaaa gtcaaaaact ggctggagcg
    23461 ggcgctgcaa tcggaacgca atattaaaga gcagattgcc gtcctgaagg gcagcctgct
    23521 gttgtctcgt atcctttacc agcaacaaca aacgctgccc tcggcggatg aactggaaaa
    23581 catgaccaac cgcatcgcgg atttgcgtct cgaacagttt gaagttaacc agcagcgtga
    23641 tgcactcttc cagagcgatg cgttcgtcaa caaactggaa gaaggtcaca ccaacgaagt
    23701 caacagcgaa gttcacgatg cgttattgca agtggttgat atgcgtcgcg aattgctgga
    23761 tcaactcaac aaacagttgg gtaaccagct gatgatggcc attaacctgc aaatcaacca
    23821 gcagcagtta atgagcgtat cgaaaaacct gaaatccatc ctgactcagc aaatcttttg
    23881 ggtgaacagt aaccgtccaa tggactggga ctggatcaaa gcgttcccgc aaagcctgaa
    23941 agatgaattt aagtcgatga aaatcacggt gaactgggaa aaagcctggc ctgccgtttt
    24001 tatcgctttc ctcgccggtt tgccgctgct gttgattgcc gggctgatcc actggcgtct
    24061 gggctggctg aaagcgtatc aacagaaact ggcttccgct gtgggttccc tgcgtaacga
    24121 tagccagctc aacacaccaa aagcgatcct tatcgacctg atccgcgcgc tgccggtgtg
    24181 cctgattatt ctcgcggttg gcctgattct gttgaccatg cagctcaaca tcagcgaact
    24241 gctatggtcg ttcagtaaaa aactggcgat tttctggctg gtgtttggcc tgtgctggaa
    24301 ggtactggag aaaaatggcg ttgccgtgcg tcacttcggc atgccggagc agcagaccag
    24361 ccactggcgt cggcaaattg tccgcatcag tctcgcgttg ctgcctatcc atttctggtc
    24421 tgtggtggca gaactttccc cgctgcatct gatggatgat gtgctggggc aggcgatgat
    24481 tttcttcaac ctgctgctga ttgccttcct ggtatggcca atgtgccgcg aaagctggcg
    24541 tgataaagag tcgcacacca tgcgactggt caccattacc gtgctgtcga ttatcccgat
    24601 tgcattgatg gtgctgactg ccacgggtta tttctacacc acgctgcgcc tctccgggcg
    24661 ttggattgaa accgtttatc tggtgatcat ctggaacttg ctctaccaga cggtattgcg
    24721 cggcttaagc gtagcggcgc ggcgtatcgc ctggcgtcgt gcgctggcgc gtcggcagaa
    24781 tctggtgaaa gagggcgcag aaggtgccga accgccggaa gaacccacca ttgcactgga
    24841 acaggttaac cagcagacgc tgcgtattac catgttgctg atgtttgcgc tgttcggtgt
    24901 catgttctgg gcaatttggt ccgatttgat caccgtgttc agctatctcg acagcatcac
    24961 gctctggcat tacaacggca ctgaagctgg cgctgcggtg gtgaaaaacg tcaccatggg
    25021 cagtctgttg tttgcgatta tcgcctcaat ggtggcctgg gcgttgattc gcaacctgcc
    25081 tggtttactg gaagtgctgg tgctctcgcg actgaatatg cgtcagggcg cgtcgtatgc
    25141 cattactacc atccttaact acatcattat tgccgttggt gcgatgacgg tgttcggatc
    25201 gctgggcgtc tcttgggata aactccagtg gctggccgca gcattatccg taggtcttgg
    25261 ttttggttta caagaaattt tcggtaactt cgtctccggt ttgatcattc tgttcgaacg
    25321 tccggtgcgt attggcgata cggtaaccat tggtagcttc tcggggacgg taagtaagat
    25381 ccgtattcgt gcgacaacga ttaccgattt cgatcgcaaa gaagtgatca tcccgaacaa
    25441 agcgtttgtt accgagcgtc tgatcaactg gtcgttgact gacactacta cgcgtctggt
    25501 gatccgtctc ggcgtggcct atggctccga tctggaaaaa gtgcgtaaag tgttactgaa
    25561 ggcggcgact gagcacccaa gggtgatgca cgaaccaatg ccggaagtct tctttacggc
    25621 gtttggtgcc agcacgttgg atcatgagct gcgtctgtat gtgcgtgaac tgcgtgaccg
    25681 tagtcgtact gtcgatgagc tgaaccgtac tatcgatcag ctgtgccgtg aaaacgacat
    25741 caacattgcc tttaaccagc ttgaagtgca tctgcacaac gagaagggcg atgaggtgac
    25801 ggaagtcaaa cgcgactaca aaggcgatga cccgacgcca gcggtagggt aataaccaaa
    25861 gggggcgaca tttagttgcc ccgagattac tgacaaagtg cgcgttgttc atgccggatg
    25921 cggcgtgacc gccttatccg gcctacgaaa ccgcaagaat tcaatgtatt gcaggagcgg
    25981 tgtaggcctg ataagcgtag cgcatcaggc agttttgcgt ttgcccacaa tcttagggga
    26041 catttagcgg ccccatttat ttctcacttt tccgcctcat catcgcgcgt taatttcttt
    26101 tcataatcgc gttttacaat atccagcgcg cgcagcacgg tgcgggctgg gatctgattt
    26161 tcttccagca gcacaatcaa atcgacagcc agtttgacat cgtcaggggc attttcaagt
    26221 gacatactct ctccattgct agcgggttaa gcgcgctagc ctgttttcga ttttttccag
    26281 cgcatggcgg cagcgtgcca ggcgcgcttc ataggcttcc acttcacgat gcagcgtttg
    26341 ctgttccacg agatcggtca cccgcgccag gcgggctctg cgttcggcaa ccatctcacg
    26401 tagccgccgc tcaaaatcct gatgctgaat acgtttacgc tgccagcggg caattttcgg
    26461 tggcgcactg tcccactcgc gcaatgacca ggcggaggct tcacgcgcga tggcttccag
    26521 ttgtgccgcc agatgttccg ccagccaggc cacttgcggc agctgttgct gctcaaccgc
    26581 atgacaaagc gcagccagat tatcgcccgc ctcgtcgaga caagcctgta gtgttgtcgc
    26641 acgagtctga aaaagatgcc tgtcgaaacg agcacttagc gtggcgaact gtgacaccgg
    26701 ggcacaacgc tgacgcagcg tagcgagctg tccttccagt ttttccagca gcagggcggt
    26761 tttcacgata agacctcaat gaaaatgata attgttatgc taaagtagcc actctgtaag
    26821 ctgacaacac tcaatatgca acgaatcatt ttaatcatca ttggctggct ggcggtagtg
    26881 ctgggtacgc tgggcgtggt attaccggta ttaccgacga cgccgtttat cctgctggcg
    26941 gcctggtgct ttgcccgttc ttccccgcgc tttcacgcct ggttgctgta ccgctcatgg
    27001 tttggcagct atctacgttt ctggcagaaa catcatgcga tgccgcgcgg cgtcaaaccg
    27061 cgggcgattt tgcttatttt gctcacgttt gccatttctc tgtggttcgt ccagatgcca
    27121 tgggtgcgca tcatgttgct ggtaattctc gcctgtttgc ttttctatat gtggcgaatt
    27181 ccggtgattg atgaaaagca agaaaagcac tgaagcacaa caatcgcagt tgcaattatt
    27241 gcgtacagcc agtacattct ggcgttttcg agcacaggcg caggcggtca aaggttaaac
    27301 aactgttact tttgatacgt ttaaaacgcg ccgtgagtac caccgtaaca agcaggcata
    27361 cacttatgac cgcgactgca cagcagcttg agtatctcaa aaatagcatc aaaagcattc
    27421 aggactaccc aaaacccggc attcttttcc gcgatgtcac cagcttactg gaagacccga
    27481 aagcttacgc tctcagcatc gacttgctgg ttgagcgtta caaaaatgcg ggcattacca
    27541 aagttgtcgg caccgaagcg cgtggcttct tgtttggcgc tccggtagct ctgggtctgg
    27601 gcgttggctt tgtaccggtc cgtaaaccgg gcaaactgcc gcgtgaaacc atcagtgaaa
    27661 cttacgacct ggaatacggc accgatcagc tggagatcca cgttgatgcc atcaaaccgg
    27721 gcgacaaagt tctggtggtg gacgatctgc tggcaaccgg cggcactatc gaagcgaccg
    27781 ttaaactgat ccgtcgtctg ggtggtgaag tggctgacgc tgcgttcatt atcaacctgt
    27841 tcgatctcgg cggcgaacag cgtctcgaaa aacagggcat taccagctac agccttgtcc
    27901 cgttcccggg ccattaatta tcgccagtct tgtgctgccc acgctacgga cagcacaaga
    27961 tgtgcattca gcctcgccgt tctgacgggg ctgtgttagc attacccctt cgtgaatcca
    28021 ccttccagcg tttcagagcc tgccaatgag ttatcaggtc ttagcccgaa aatggcgccc
    28081 acaaaccttt gctgacgtcg tcggccagga acatgtgctg accgcactgg cgaacggctt
    28141 gtcgttaggg cgtattcatc atgcttatct tttttccggc acccgtggcg tcggaaaaac
    28201 ctctatcgcc cgactgctgg cgaaggggct aaactgcgaa accggcatta ccgcgacgcc
    28261 gtgcggcgtg tgcgataact gtcgtgaaat cgagcagggg cgctttgtcg atctgattga
    28321 aatcgacgcc gcctcgcgca ccaaagttga agatacccgc gacctgctgg ataacgtcca
    28381 gtacgctccg gcgcgtggtc gtttcaaagt ttatctgatc gacgaagtgc atatgctgtc
    28441 gcgccacagc tttaacgcac tgttaaaaac ccttgaagag ccgccggagc acgttaagtt
    28501 tctgctggcg acgaccgatc cacagaaatt gccggtgacg attttgtcgc gctgtttgca
    28561 atttcatctc aaggcgctgg atgtcgagca aattcgccat caacttgagc acatcctcaa
    28621 cgaagaacat atcgctcacg agccgcgggc gttgcaattg ctggcgcgcg ccgctgaagg
    28681 cagcctgcga gatgccttaa gtctgaccga ccaggcgatt gccagcggtg acggccaggt
    28741 ttcaacccag gcagtcagtg cgatgctggg tacgcttgat gacgatcagg cgctgtcgct
    28801 ggttgaagcg atggtcgagg ccaacggcga gcgcgtaatg gcgctgatta atgaagccgc
    28861 tgcccgtggt atcgagtggg aagcgttgct ggtggaaatg ctcggcctgt tgcatcgtat
    28921 tgcgatggta caactttcgc ctgctgcact tggcaacgac atgcccgcca ttgagctgcg
    28981 gatgcgtgaa ctggcgcgca ccataccgcc gacggatatt cagctttact atcagacgct
    29041 gttgattggt cgcaaagaat taccgtatgc gccggaccgc cgcatgggcg ttgagatgac
    29101 gctgctgcgc gcgctggcgt tccatccgcg tatgccgctg cctgagccag aagtgccacg
    29161 ccagtccttt gcacccgtcg tgccaacggc agtaatgacg ccaacccagg tgccgccgca
    29221 atcagcgccg cagcaggcac cgactgtacc gctcccggaa accaccagcc aggtgctggc
    29281 ggcgcgccag cagttgcagt gcgtgcaggg agcaaccaaa gcaaaaaaga gtgaatcggc
    29341 agccgctacc cgcgcgcggc cggtgaataa cgctgcgctg gaaagactgg cttcggtcac
    29401 cgatcgcgtt caggcgcgtc cggtgccatc ggcgctggaa aaagcgtcag ccaaaaaaga
    29461 agcgtatcgc tggaaggcga ccactccggt gatgcagcaa aaagaagtgg tcgccacgcc
    29521 gaaggcgctg aaaaaagcgc tggaacatga aaaaacgccg gaactggcgg cgaagctagc
    29581 ggcagaagcc attgagcgcg acccgtgggc ggcacaggtg agccaacttt cgctaccaaa
    29641 actggtcgaa caggtggcgt taaatgcctg gaaagaggag agcgacaacg cagtatgtct
    29701 gcatttgcgc tcctctcagc ggcatttgaa caaccgcggt gcacagcaaa aactggctga
    29761 agcgttgagc atgttaaaag gttcaacggt tgaactgact atcgttgaag atgataatcc
    29821 cgcggtgcgt acgccgctgg agtggcgtca ggcgatatac gaagaaaaac ttgcgcaggc
    29881 gcgcgagtcc attattgcgg ataataatat tcagaccctg cgtcggtttt tcgatgcgga
    29941 gctggatgaa gaaagtatcc gccccatttg atcgtaagca cagcttacgt tcgtcatcct
    30001 taacgtgatt gagagagaaa cctatgtttg gtaaaggcgg tctgggtaac ctgatgaagc
    30061 aagcccagca gatgcaagaa aaaatgcaga aaatgcagga agagatcgcg cagctggaag
    30121 tcaccggcga atctggcgca ggtctggtaa aagtgaccat caacggtgca cacaactgcc
    30181 gtcgcgtaga gatcgacccg agcctgctgg aagacgacaa agagatgctg gaagacctgg
    30241 tggctgcagc attcaacgac gcagcacgtc gtattgaaga aacgcagaaa gaaaaaatgg
    30301 cctctgtatc ctccggaatg cagctgccgc ctggctttaa gatgccgttc tgatgcaaac
    30361 cagcccgctg ttaacacagc ttatggaagc actgcgctgt ctgccgggcg ttggcccgaa
    30421 gtcggcgcag cgtatggcgt tcacgctgct tcagcgcgat cgtagcggcg ggatgcgttt
    30481 ggcgcaggcg ctcacccggg cgatgtcgga aatcggccac tgcgccgatt gccgcacttt
    30541 caccgaacag gaagtctgta acatctgttc gaatccgcgt cgtcaggaaa acggtcaaat
    30601 ctgtgtggtg gagagtccgg cggacatcta cgccattgag cagacggggc agttttctgg
    30661 tcgttatttt gtgttgatgg ggcatctgtc accgctggac ggcatcggtc cggatgatat
    30721 cgggcttgat cgtctggaac agcgtctggc agaggaaaaa atcactgaag tgatcctcgc
    30781 caccaatccc acggttgaag gtgaagctac cgctaactac attgccgagc tttgcgcgca
    30841 atatgacgtg gaagccagcc gaatcgctca tggcgtaccg gttggcggcg aactggaaat
    30901 ggtcgatggc accacgctgt cacactccct tgccgggcgt cataagattc gtttttaagc
    30961 aaacgagagc aggatcacct gctctcgctt gaaattattc tcccttgtcc ccatctctcc
    31021 ctcatcctgt ttttaacctt aaaatggcat tattgaggta gacctacatg aaaggacaag
    31081 aaactcgtgg ttttcagtca gaagtgaaac agcttctgca cctgatgatc cattctctct
    31141 attccaataa agaaatcttc ctgcgtgagc ttatctctaa cgcctccgat gcggcggaca
    31201 agctgcgttt ccgtgcgcta tctaacccgg acctgtacga aggtgatggc gaactgcgcg
    31261 ttcgtgtctc tttcgataaa gacaagcgca cgctgaccat ctctgataac ggcgtgggga
    31321 tgacccgcga cgaagtgatt gaccatctgg ggactatcgc taaatccggt accaaatcat
    31381 tcctcgaatc cctgggttct gaccaggcga aagacagcca gctgatcggt cagtttggtg
    31441 ttggtttcta ctctgcgttt atcgtggccg acaaagtgac cgtgcgtact cgcgcggcag
    31501 gcgaaaaacc agaaaacggc gtcttctggg aatcggctgg cgaaggtgaa tataccgttg
    31561 ccgacatcac caaagaagat cgtggtactg aaatcaccct gcatctgcgt gaaggcgaag
    31621 acgagttcct cgatgactgg cgcgtgcgtt ccatcatcag caaatactcc gaccatatcg
    31681 cgctgccggt agagatcgaa aaacgcgaag agaaagacgg cgaaaccgtt atctcctggg
    31741 agaaaatcaa caaagcgcag gcgctgtgga ctcgtaacaa gtcggaaatc accgatgaag
    31801 agtacaaaga gttctacaaa cacatcgccc acgactttaa tgatccgctg acctggagcc
    31861 acaaccgcgt tgaaggtaag caggagtaca ccagcctgct gtacattccg tcccaggctc
    31921 cgtgggatat gtggaaccgt gatcataaac acggcctgaa actgtacgtc cagcgtgtgt
    31981 tcatcatgga cgacgcagaa cagttcatgc cgaactatct gcgcttcgtg cgtggtctga
    32041 ttgactccag cgatctgccg ctgaacgttt cccgtgaaat cctccaggac agcacggtaa
    32101 cgcgtaacct gcgcaatgcg ctgaccaagc gtgtgctgca aatgctggaa aaactggcga
    32161 aagacgacgc ggaaaaatac cagaccttct ggcaacagtt tggcctggta ctgaaagaag
    32221 gtccggcgga agatttcgct aaccaggaag cgatcgccaa actgctgcgt tttgcttcta
    32281 cccataccga ttcttctgcg cagaccgtat ctctggaaga ctacgtttcc cgcatgaaag
    32341 aagggcagga gaaaatctac tacatcaccg cagacagcta tgcggcagcg aagagcagcc
    32401 cgcacctgga actgctgcgt aagaaaggca tcgaagttct gctgctttcc gaccgcatcg
    32461 atgagtggat gatgaactat ctgactgagt tcgacggtaa accgttccag tcggtgtcta
    32521 aagttgacga gtcgctggaa aaactggctg acgaagttga tgagagcgcg aaagaagcgg
    32581 agaaagcact gactccgttc atcgaccgtg tgaaagccct gctcggcgag cgcgtgaaag
    32641 atgtccgtct gactcaccgt ctgaccgata cgccagcgat cgtttcgacc gacgcggacg
    32701 aaatgagcac tcagatggcg aaactgttcg ctgcggcggg ccagaaagtg ccagaagtga
    32761 aatacatctt cgaactgaac ccggatcacg tactggtgaa acgtgcggca gatactgaag
    32821 atgaagcgaa gttcagcgag tgggtagaac tgctgctgga tcaggcgctg ctggcagaac
    32881 gcggcacgct ggaagatccg aacctgttta ttcgtcgtat gaaccagctg ctggtttcct
    32941 gatgtcatgc cggatgacct ttgtgtcatc cggcattttt cttttcatca tctgcacttt
    33001 ccgcaaatta tctcgccatt aaccgtttca gcctcaggtg cctttcttga ggcaatcgcc
    33061 tgttggtggt atcgtttatc gctttttcaa aaaaatcgac acattttaag gggattttcg
    33121 caatgcgtat cattctgctt ggcgctccgg gcgcggggaa agggactcag gctcagttca
    33181 tcatggagaa atatggtatt ccgcaaatct ccactggcga tatgctgcgt gctgcggtca
    33241 aatctggctc cgagctgggt aaacaagcaa aagacattat ggatgctggc aaactggtca
    33301 ccgacgaact ggtgatcgcg ctggttaaag agcgcattgc tcaggaagac tgccgtaatg
    33361 gtttcctgtt ggacggcttc ccgcgtacca ttccgcaggc agacgcgatg aaagaagcgg
    33421 gcatcaatgt tgattacgtt ctggaattcg acgtaccgga cgaactgatt gttgatcgta
    33481 tcgtaggccg ccgcgttcat gcgccgtctg gtcgtgttta tcacgttaaa ttcaatccgc
    33541 cgaaagtaga aggcaaagac gacgttaccg gtgaagaact gactacccgt aaagacgatc
    33601 aggaagaaac cgtgcgtaaa cgtctggttg aataccatca gatgactgca ccgctgatcg
    33661 gctactactt caaagaagcg gaagcgggta acaccaaata cgcgaaagtt gacggcacca
    33721 agtcggttgc tgaagttcgc gctgatctgg aaaaaatcct cggctaatca agccgcagca
    33781 gatctgcttt cgggcaggtc tgctcatcca cctcaggcaa tacctctcac agcaattagt
    33841 tcttcttcct cacttttccg ctacaattat caacaagttg aatcgataag aggcggtaat
    33901 gcgtcagact aaaatcggta tcctgctggc aaacctgggt acgcccgata cccccacacc
    33961 tgaagcggta aaacgctatc tgaaacaatt tttaagcgac agacgcgtgg ttgatacctc
    34021 acggttgtta tggtggccat tgctgcgcgg cgttattttg ccgctgcgct cgccgcgtgt
    34081 ggcgaagctg tacgcctcgg tctggatgga aggtggctcg ccgctgatgg tttacagccg
    34141 ccagcaacag caggcgctgg cacaacgttt accggagacg cccgtagcgc tgggaatgag
    34201 ctacggctcg ccatcactgg aaagcgccgt cgatgaactc ctggcagagc atgtagatca
    34261 tattgtggtg ctgccgcttt atccgcaatt ctcctgttct acggtcggtg cggtatggga
    34321 tgaactggca cgcattctgg cgcgcaaacg tagcattccg gggatatcgt ttattcgtga
    34381 ttacgccgat aaccacgatt acattaatgc actggcgaac agcgtacgcg cttcttttgc
    34441 caaacatggc gaaccggatc tgctgctgct ctcttatcat ggcattcccc agcgttatgc
    34501 agatgaaggc gatgattacc cgcaacgttg ccgcacaacg actcgcgaac tggcttccgc
    34561 actggggatg gcaccggaaa aagtgatgat gacctttcag tcgcgctttg gccgggaacc
    34621 ctggctgatg ccttataccg acgaaacgct gaaaatgctc ggagaaaaag gcgtaggtca
    34681 tatacaggtg atgtgcccgg gctttgctgc ggattgtctg gagacgctgg aagagattgc
    34741 cgagcaaaac cgtgaggtct tcctcggtgc gggcgggaaa aaatatgaat atattccagc
    34801 gcttaatgcc acgccggaac atatcgaaat gatggctaat cttgttgccg cgtatcgcta
    34861 aagctgagcg gtaaagaact gagcgccgtc gcgaagagcc tcgtcggcgg ttttcatcat
    34921 ccgtgaataa tgcaaaaagg catgcagcgt acctgggtag agtttgaact cacagggctg
    34981 ctgatgcgcc gctaacgtct ggtaaagcag acggctgtca tccagcagcg gatcgaactc
    35041 cgccccggca ataaaacagg gcggaacttc gcgagtgaga tcattattaa acagacagta
    35101 atacggtgac tcgcggtccg cgtcgttgct taaatacgcc tcttcataca tctgcaaatc
    35161 ctgttgcgtt aagccatccc agacaccgcc caacagacga cgagtcacgg aatcccgtaa
    35221 tccgtaaagc ccataccaca gcaaaacacc cgcaatttta ccgcaatcga tctgtttatc
    35281 acgcaaccac aacgcactgg cgagcgccag catggcacct gcggaatcac cggcaaagcc
    35341 aatgcgggac atattgattt gataatcctc cgcctgctgg tggaaataac aacaagcagc
    35401 cacaatttcc tctatcgctt gcggaaaacg cgcttcaggt gaaagggtgt aatcaatacc
    35461 aatcaccgta cattggctgt agcttgccag caggcgcatg atgcgatcgt gggtatcgag
    35521 attgccgaga ataaaaccgc ctccatgcaa ataaaatagc gtcgccgggc tatctggctg
    35581 cggacaaaag agacgtgttt ccacctgccc atattttgtt ggaaccatgt aagctctggt
    35641 tgccatttct ggagcgcccg cattccagaa tcggcgctca agcgtgtaat actgtcgttg
    35701 ctcagcaatc gttcccgttg cgggccagga cggtaaatcc ggctgaagag tattcacaac
    35761 ggtcttcatt tcagcagaaa taaggtccag aacaggtagt ttgttttccg gcttcataca
    35821 aaaactcctt tcaaattacg tcattgtaag gaaccactgc catgaaaatg cgatcccgcc
    35881 tgctgatatt gaaactggct gcgtctcgcg cgctcccgtc agattgtgtt aacattcgcc
    35941 gctcagttaa ccacccgtaa aaacaaccat gaaatttccc ggtaaacgta aatccaaaca
    36001 ttacttccct gtaaatgcac gcgatccgct gcttcagcaa ttccagccag aaaacgaaac
    36061 cagcgccgcc tgggtcgtag gtattgacca gacgctggtc gatattgaag cgaaagtgga
    36121 tgatgaattc attgagcgtt atggattaag cgccgggcat tcactggtga ttgaggatga
    36181 cgtagccgaa gcgctttatc aggagctaaa acagaaaaac ctgattaccc atcagtttgc
    36241 gggtggcacc attggtaaca ccatgcacaa ctactcggtg ctcgcggacg accgttcggt
    36301 gctgctgggc gtcatgtgca gcaatattga aattggcagt tatgcctatc gttacctgtg
    36361 taacacttcc agccgtaccg atcttaacta tctacaaggc gtggatggcc cgattggtcg
    36421 ttgctttacg ctgattggcg agtccgggga acgtaccttt gctatcagtc caggccacat
    36481 gaaccagctg cgggctgaaa gcattccgga agatgtgatt gccggagcct cggcactggt
    36541 tctcacctct tatctggtgc gttgcaaacc gggtgaaccc atgccggaag caaccatgaa
    36601 agccattgag tacgcgaaga aatataacgt accggtggtg ctgacgctgg gcaccaagtt
    36661 tgtcattgcc gagaatccgc agtggtggca gcaattcctc aaagatcacg tctctatcct
    36721 tgcgatgaac gaagatgaag ccgaagcgtt gaccggagaa agcgatccgt tgttggcatc
    36781 tgacaaggcg ctggactggg tagatctggt gctgtgcacc gccgggccaa tcggcttgta
    36841 tatggcgggc tttaccgaag acgaagcgaa acgtaaaacc cagcatccgc tgctgccggg
    36901 cgctatagcg gaattcaacc agtatgagtt tagccgcgcc atgcgccaca aggattgcca
    36961 gaatccgctg cgtgtctatt cgcacattgc gccgtacatg ggcgggccgg aaaaaatcat
    37021 gaacactaat ggagcggggg atggcgcatt ggcagcgttg ctgcatgaca ttaccgccaa
    37081 cagctaccat cgtagcaacg taccaaactc cagcaaacat aaattcacct ggttaactta
    37141 ttcatcgtta gcgcaggtgt gtaaatatgc taaccgtgtg agctatcagg tactgaacca
    37201 gcattcacct cgtttaacgc gcggcttgcc ggagcgtgaa gacagcctgg aagagtctta
    37261 ctgggatcgt taagttatcg tcggttcgta ggccagataa ggcgttcacg ccgcatctgg
    37321 catttggctc tcgatgcctg atgcgacgct ggcgcgtctt atcatgccta catatttttc
    37381 atattttaca tccggcaacc accgtttacc ccgtcaccac ttcacccgcc ggtggcgttt
    37441 ccagcagttc cagcatagta cgggcaattt cacgttcacc catcactacc tgattcgcgc
    37501 cacgttcggt gatatacgtc acttcatcgt cataatgggc gcgggcgata atctcaatat
    37561 ccgggttttt cgcgcgggca gacgccacaa tctcacccgc ttcataaccg ttggggatcg
    37621 tcaggatcag ccattttgca cattccagat gcgccagttg cataatttct tcgttcgctg
    37681 cattgcccaa tacagcgcgg accccacgct ctcgcagctc atcaacacgg gttcgtgacg
    37741 tctcaatcac caccagcgga atatcagagg cgagcaattt ctcccccagc aggctgccta
    37801 cacgaccgta acccaccaat agcgcatggt tgcaaatatc cactgggatc tgtttctctt
    37861 cttcgattgc ctcttccagc gtctgctctt ccagcgtttc ggtcttcgcc agatatttct
    37921 ccagtagtgc gaacagtacc gggttgagca taatcgacag gatcgcccct gccagtacca
    37981 ggttttgtcc ggcctgcggc agtaaattca atgccattcc cagtcccgcc aggataaacg
    38041 cgaactcacc aatctgcgcc aggctggcgg cgatggttaa tgccgtacgt tgggagtgac
    38101 caaacagacg gacaaggaac agggccgcga ccgatttacc aaacagaata attgccagcg
    38161 tcgccagcac tgccagcggt tgctgaatca gaattaacgg atcaaacaac atcccgacgg
    38221 agacaaaaaa cagcaccgca aacgcgtcgc gcaatggcag cgtatcgtgg gcggcacggt
    38281 gactcagttc agactcgttc agtaccatcc cggcaaagaa cgcgccaagt gcgaaggaga
    38341 cgtcaaacaa ctccaccgca ccaaaggcaa tccctaacgc cagcgccagc accgacaggg
    38401 taaatagctc gcgagaaccg gttgccgcgc tgcgtgccat aatccacggc accagacggc
    38461 gacctaccag catcataatg gcgataaatg cgatcacttt gccgatggtg atccccatat
    38521 cgactgcaag agtggcaaag cccacatcgc cctgttccat cattcctgcc actgcgggca
    38581 gcaacaccag cgtcagaacc attaccaggt cttccacaat caaccaaccg atggcgattt
    38641 gcccacgctg actgtcaatt aattgccgtt cttcaagtgc gcgcagtaac accacggtac
    38701 tggcggtgga aagacataaa ccgaacacga taccggtcat taacgaccag cccagcacgg
    38761 cagagagcgc catacccagc agcgtcgcca cggctatctg ggcgatcgca ccgggaatgg
    38821 cgatggcctt taccgccatc aaatccttca gcgaaaagtg caaaccgacg ccaaacatca
    38881 acagaatgac gcccagttca gccagttccg gcgcaagctt ggtatcggca acaaagcccg
    38941 gagtgaatgg tcctgccagc acacccgcta acagatatcc caccagagga gaaatacgta
    39001 gtttattggc cagcatgccg aggataaagg cgagcacaag gccgccaaca atggtggtga
    39061 taagcggggt ggcgtgatgc attccgtctc cttttcctgg tggttattgt ccatttttgg
    39121 ccgggaaaac caaaattaca ggtaatagtt tatgacaatt tcattgatga tgttcatgaa
    39181 taattattga attttgcaga aaaatggaat tagctgcaaa aaaagcacgg atcggaaaac
    39241 agaaggcggt ttaacagagg aaaggcttat ggcgactgta aaacgatgca gccaaagttt
    39301 gcctttggct gcaatgaaat cagtctttat gccggttatc aggcaggaat atggtcaaca
    39361 tccccaatag tggcaggaaa gcacagattt tatagactaa ctcgatgct
//
LOCUS       DAZPDU010000019        38874 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZPDU010000019 DAZPDU010000000
VERSION     DAZPDU010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38874
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.019"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            67..1116
                     /locus_tag="ACHURS_000895"
     CDS             67..1116
                     /locus_tag="ACHURS_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFR1236693.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATESVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            1190..2047
                     /gene="nfo"
                     /locus_tag="ACHURS_000896"
     CDS             1190..2047
                     /gene="nfo"
                     /locus_tag="ACHURS_000896"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFR1236694.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            2051..3139
                     /locus_tag="ACHURS_000897"
     CDS             2051..3139
                     /locus_tag="ACHURS_000897"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFR1236695.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(3195..4445)
                     /locus_tag="ACHURS_000898"
     CDS             complement(3195..4445)
                     /locus_tag="ACHURS_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFR1236696.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(4545..5486)
                     /gene="rihB"
                     /locus_tag="ACHURS_000899"
     CDS             complement(4545..5486)
                     /gene="rihB"
                     /locus_tag="ACHURS_000899"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFR1236697.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGEMFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            5616..6314
                     /locus_tag="ACHURS_000900"
     CDS             5616..6314
                     /locus_tag="ACHURS_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFR1236698.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(6381..7631)
                     /locus_tag="ACHURS_000901"
     CDS             complement(6381..7631)
                     /locus_tag="ACHURS_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFR1236699.1"
                     /translation="MDIMRSVAGMVVLLAIAFVLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(7725..8663)
                     /gene="psuG"
                     /locus_tag="ACHURS_000902"
     CDS             complement(7725..8663)
                     /gene="psuG"
                     /locus_tag="ACHURS_000902"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFR1236700.1"
                     /translation="MSELTLSPELLQISAEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSAREIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEETINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLVG"
     gene            complement(8651..9592)
                     /locus_tag="ACHURS_000903"
     CDS             complement(8651..9592)
                     /locus_tag="ACHURS_000903"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFR1236701.1"
                     /translation="MREKDYIVIIGSANIDVAGYSHHPLNYADSNPGKIKFTPGGVGR
                     NIAHNLALLGKNAWLLSAVGGDFYGQSLLAQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMSISDCISAEFLAQHQEFIRGAKVIVADCNLSEEALVWVLENSGETP
                     VFIDPVSAWKCVKIRDHLSKIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDINGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFIDSV
                     RFAQGCSSMALACEYTNNPELSIANVRSLVENTECLN"
     gene            complement(10050..11741)
                     /gene="fruA"
                     /locus_tag="ACHURS_000904"
     CDS             complement(10050..11741)
                     /gene="fruA"
                     /locus_tag="ACHURS_000904"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFR1236702.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILDGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPETQIVEKNA"
     gene            complement(11758..12696)
                     /gene="fruK"
                     /locus_tag="ACHURS_000905"
     CDS             complement(11758..12696)
                     /gene="fruK"
                     /locus_tag="ACHURS_000905"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFR1236703.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTLADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(12696..13826)
                     /gene="fruB"
                     /locus_tag="ACHURS_000906"
     CDS             complement(12696..13826)
                     /gene="fruB"
                     /locus_tag="ACHURS_000906"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFR1236704.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEVGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            14194..15375
                     /gene="setB"
                     /locus_tag="ACHURS_000907"
     CDS             14194..15375
                     /gene="setB"
                     /locus_tag="ACHURS_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFR1236705.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLADVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(15372..15626)
                     /locus_tag="ACHURS_000908"
     CDS             complement(15372..15626)
                     /locus_tag="ACHURS_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFR1236706.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            15781..16353
                     /gene="yeiP"
                     /locus_tag="ACHURS_000909"
     CDS             15781..16353
                     /gene="yeiP"
                     /locus_tag="ACHURS_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFR1236707.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            16576..18042
                     /locus_tag="ACHURS_000910"
     CDS             16576..18042
                     /locus_tag="ACHURS_000910"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFR1236708.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKCSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFVHISDCMQDRVFRHAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            18160..19146
                     /gene="yeiR"
                     /locus_tag="ACHURS_000911"
     CDS             18160..19146
                     /gene="yeiR"
                     /locus_tag="ACHURS_000911"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFR1236709.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYELWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            19185..19898
                     /gene="lpxT"
                     /locus_tag="ACHURS_000912"
     CDS             19185..19898
                     /gene="lpxT"
                     /locus_tag="ACHURS_000912"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFR1236710.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            20310..20876
                     /gene="mepS"
                     /locus_tag="ACHURS_000913"
     CDS             20310..20876
                     /gene="mepS"
                     /locus_tag="ACHURS_000913"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFR1236711.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            21057..22613
                     /locus_tag="ACHURS_000914"
     CDS             21057..22613
                     /locus_tag="ACHURS_000914"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFR1236712.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            22695..24509
                     /locus_tag="ACHURS_000915"
     CDS             22695..24509
                     /locus_tag="ACHURS_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFR1236713.1"
                     /translation="MIVRILLLFIALFTFGVQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPSKEDMLSLFSLPIFPEKYWKDHKLSDPLATPPLASGPYRVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNETGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            24510..25604
                     /locus_tag="ACHURS_000916"
     CDS             24510..25604
                     /locus_tag="ACHURS_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFR1236714.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            25604..26629
                     /locus_tag="ACHURS_000917"
     CDS             25604..26629
                     /locus_tag="ACHURS_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1236715.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            26631..28220
                     /gene="yejF"
                     /locus_tag="ACHURS_000918"
     CDS             26631..28220
                     /gene="yejF"
                     /locus_tag="ACHURS_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFR1236716.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(28224..28568)
                     /locus_tag="ACHURS_000919"
     CDS             complement(28224..28568)
                     /locus_tag="ACHURS_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFR1236717.1"
                     /translation="MTSLQLSIVHRLPQNFRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(28901..29958)
                     /locus_tag="ACHURS_000920"
                     /pseudo
     CDS             complement(28901..29958)
                     /locus_tag="ACHURS_000920"
                     /inference="COORDINATES: protein motif:HMM:NF008314.0"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
     gene            complement(30049..30744)
                     /gene="rsuA"
                     /locus_tag="ACHURS_000921"
     CDS             complement(30049..30744)
                     /gene="rsuA"
                     /locus_tag="ACHURS_000921"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFR1236718.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLVSPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            30894..32654
                     /locus_tag="ACHURS_000922"
     CDS             30894..32654
                     /locus_tag="ACHURS_000922"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFR1236719.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NIDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRSDEKALFRDCIYELPLRYMIKHSYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIENFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRPPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            32779..33063
                     /gene="rplY"
                     /locus_tag="ACHURS_000923"
     CDS             32779..33063
                     /gene="rplY"
                     /locus_tag="ACHURS_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFR1236720.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(33202..34209)
                     /gene="yejK"
                     /locus_tag="ACHURS_000924"
     CDS             complement(33202..34209)
                     /gene="yejK"
                     /locus_tag="ACHURS_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFR1236721.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNTKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWEPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            34391..34618
                     /locus_tag="ACHURS_000925"
     CDS             34391..34618
                     /locus_tag="ACHURS_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFR1236722.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            34638..36398
                     /gene="yejM"
                     /locus_tag="ACHURS_000926"
     CDS             34638..36398
                     /gene="yejM"
                     /locus_tag="ACHURS_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFR1236723.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGHYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            36473..36549
                     /locus_tag="ACHURS_000927"
     tRNA            36473..36549
                     /locus_tag="ACHURS_000927"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:36507..36509,aa:Pro,seq:ggg)
     gene            complement(36652..38874)
                     /gene="yejO"
                     /locus_tag="ACHURS_000928"
                     /pseudo
     CDS             complement(36652..38874)
                     /gene="yejO"
                     /locus_tag="ACHURS_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter YejO"
BASE COUNT         9561 a        10132 c         9423 g         9758 t
ORIGIN      
        1 atatataatc aattttattt ttaaaccagt tagtcgtaac cttatgcccg gttaaaggag
       61 agggttatga cgaatatcac cttacagaaa caacatcgta cactgtggca ttttattccg
      121 gggttagccc tgagtgcagt tatcaccggg gtcgccctgt ggggtggttc cattcccgcg
      181 gttgcgggtg ccgggtttag tgccctcacc ctcgcaatct tgttggggat ggttttaggc
      241 aacaccatct atccgcacat ctggaaaagc tgtgacggtg gtgtgctgtt tgccaagcaa
      301 tatttattac gtctgggtat cattctttat ggcttccgtc tgacgttctc gcaaattgcc
      361 gatgtcggta tcagtgggat catcattgac gtgttgacgc tgtccagtac cttcctgctg
      421 gcttgcttcc tggggcagaa agtgtttggt ctggataagc acaccagctg gttgatcggt
      481 gccggtagca gtatctgtgg tgctgccgcg gtactggcga ctgagtcggt agtgaaagcg
      541 gaagccagta aagtaaccgt ggctgttgca accgttgtta tcttcgggac cgtcgcgatt
      601 ttcctctacc cggcgatata tccgctgatg tctcaatggt ttagtccgga aactttcggt
      661 atctacattg gttctactgt gcacgaagtg gcgcaggtgg tggcggcagg tcatgccatc
      721 agcccggatg cggaaaacgc agcagttatt tccaaaatgc tgcgcgtgat gatgctggct
      781 ccgttcctca tcctgctggc ggcgcgtgtt aaacagctgt ctggggcgaa cagcggcgag
      841 aaaagcaaaa tcactattcc gtggtttgcc atcttgttca tcgtcgttgc catctttaac
      901 tcgttccacc tgttaccgca gagcgtggtg aacatgctgg taacgctgga taccttcctg
      961 ctggcaatgg cgatggcggc gctgggtctg accactcacg tcagcgcgct gaaaaaagct
     1021 ggggcgaaac ctctgctgat ggcactggta ctgtttgcct ggctgattgt tggtggtggt
     1081 gcgattaact atgtgattca aagcgtcatc gcataaacca ctacatcttg ctcctgttaa
     1141 cccgctatca ttaccgtttt cctccagcgg gtttaacagg agtcctcgca tgaaatacat
     1201 tggagcgcac gttagtgctg ctggcggtct ggcaaatgcc gcaattcgcg ccgccgaaat
     1261 cgacgcaacc gcgtttgcct tgttcaccaa aaaccaacgt cagtggcgtg ccgcaccgct
     1321 cacgacgcaa accatcgatg aattcaaagc cgcctgtgaa aaatatcact acacatcggc
     1381 gcaaattctt ccccacgaca gttatctgat taacctcgga catccggtca ctgaagctct
     1441 ggaaaaatcg cgcgatgcct ttatagatga aatgcagcgt tgcgaacaac tggggctttc
     1501 tttgctcaac ttccaccctg gcagccatct gatgcagatt tcagaagagg attgccttgc
     1561 gcgtattgcc gaatccatca acattgcgct ggataaaact caaggtgtaa cagcggtgat
     1621 tgaaaacacc gccggtcagg gcagtaactt agggtttaaa ttcgaacatc tcgcggcgat
     1681 tatcgacggc gtggaagata aatcccgcgt cggcgtctgc attgatacct gccatgcttt
     1741 cgctgccggg tatgatttgc gtactccagc cgaatgcgaa aaaacgttcg ctgattttgc
     1801 ccgtattgtc ggctttaagt atctgcgcgg gatgcacctt aacgatgcga aaagtacctt
     1861 tggcagccgc gttgaccgcc atcatagcct cggtgaaggc aatatcggtc atgatgcgtt
     1921 ccgctggatc atgcaagacg accgtttcga cggcattccg ctgatccttg aaaccatcaa
     1981 cccggatatc tgggcagaag agatcgcctg gctgaaagcg caacaaactg aaaaagcggt
     2041 ggcctgatcg atgaacgaca gggaaaagca gattcttaaa attttacggc gtaacccgct
     2101 gattcaacag aatgaaatcg ccgatattct gcaaatcagc cgctcacgtg ttgccgctca
     2161 tattatggat ttgatgcgca agggtttgat taaaggcaaa ggatatatcc ttaccgagca
     2221 ggattactgt gtggttgtgg gtgcaatcaa tatggatatt cgcggaatgg cggatatccg
     2281 ttacccgcaa gcagcctctc atccaggttc tgttcactgt tccgcaggcg gcgtcggacg
     2341 taatatcgcg cataacctgg ctctgcttgg tcgggatgtg catttaatat cagctattgg
     2401 caacgatttt tacggcgaga cattactgga agaaacgcgc cgcgcgggcg tgaacgtttc
     2461 aaactgcatt cgtctgcatg gacacagcac tgcaacttat ctcgctatcg ctaataagca
     2521 ggaagaaact atcctggcaa tcaatgacac acatattctg caacagctaa cgcctcaact
     2581 gctcaatact tcacgggact tgattcgcca tgctggtgtg gtgctggctg attgtaatct
     2641 cacacctgaa gccctggagt gggtctttac cattgctgat gaaattccga tgtttgtcga
     2701 taccgtttcc gaatttaaag cgaacaaagt aaaaaactgg tacagccgga tccacactct
     2761 gaaaccaaca caaaatgaac tggaaatttt gtgggggcaa ccgattaaag acgacaacga
     2821 tcgcatcaga gctgtaaatt cgctgcatca acaaggtgtt aaacgcattt ttgtttatct
     2881 gaaagatgaa tctgtatttt gcagcgataa ggatggagaa caatttttgc tgaccgcgcc
     2941 agcgcatacg acagtagaca gttttggtgc tgacgatggt tttatggcgg gcctggtata
     3001 tagctttctt gaaggataca gtttccgcga cagcgcccgt tttgcggtag cctgcgcggc
     3061 aatttcgcgc gccagcggca gcttaaacaa ccctaccctg tctgccgata acgcgctttc
     3121 attagtgcca atggtgtaac aatgttgccg gattcgacgc ttgaccgcat cttatccggc
     3181 tcataaattg cgcactacgc taaaccaata aagaatcctg caatagtagc actcatcagg
     3241 ttagaaagtg tcgccgccgc cagcgcgcgt aaaccaagtt gggcgatttc cggcgcacgg
     3301 tgtggcgcaa ccgcagaaaa cgcccccacc accaccccga tagaaccaaa gttagcgaaa
     3361 ccgcacaacg cgaaagaaat aatcgccacg gttttagcat ccagagtgcc acccgtttgc
     3421 agatatggcg agaaattgag ataagcgaca aattcattga tcgccagctt ctgcccaatc
     3481 aaactcccgg caagatttgc atcactccag tcaaccccca tcacccacgc caatggggcc
     3541 aacaggtaac ctaaaatcga ctccagcgaa gcatgttcaa aaccaaacca gccgccaaca
     3601 ccgccgataa taccgttaat caacgcaatt attgcaacaa atgccataac aactgtcgct
     3661 acaccggcgg cgattttcag cccggtcatt gcccctgtcg cagcggcttc aataatgctt
     3721 tttggcggtg tttcggtgaa agagaggtta ttaaaagaaa cctgtgaaga ttccgtagcc
     3781 gggcttaaca ggcgggcaaa caagatcccg ccagggatcg ccattaacga tgccgccagc
     3841 aaatattcca caggtacgcc cagtgcggcg taaccaatca ttgtcgaacc agcaatcgag
     3901 gccatgccac tacaaatcgc tgtaaataat tcattgcgat tcagacgatc gataaagggt
     3961 ttgacgatcg ccggaatttc gttttgcccg aggaaaatgg tggtgaccgc gacgaatgac
     4021 tcgatcttgc tgatatttaa agctttctgg aagataccgc cgagaatgcg aattaagatc
     4081 cccatcacac cgatgtagta gagaatactc accagtgcag tgacgaagat aattgccggt
     4141 aataccctga aaccaaagat aaatcctgcg ccatcaaata aggtatccat tttcggtccg
     4201 accagagaac cgaagataaa tgcgctacct gcgtcgctgt acgccatcac tttgtggacg
     4261 ccaaaagcga ctttttctgc gacccaacgc cctggcggta accaaagcat aatcccgcca
     4321 atcacgacct gtaacactaa cgccgcgcca acggtacgca ggctgatctt cttcttgttt
     4381 actgacagta aaaacgcaat tgccagcagt accaccattc ccagaacact tctcatgaca
     4441 tccatagtga ttatctcttc atgtcatttt gggcgacggc tttctgccca tcagaaatag
     4501 ccgggcctgc atcttgcgat actgtcccgg cttgttatca acacttaatg ggtttttatg
     4561 tagccgcgta cgcactcttc gactaatccc cagaaccagt cggtatcaat agtgatgccg
     4621 actttagtat tggcgggctt gccaagaacg cccagctcgt cgcagacggt acgcccatag
     4681 caagggccac tgttgacgtc cacttcgacg tacatctcct gggttttaat gccatcaggg
     4741 ttaatcagat aaccgatgca ggtggcgtcg tgcaccgggc cgccagccag gccgtagttt
     4801 tcgaactgcg ttttgagagt gaagttcatg atgtcgctga acatctctcc ggcagggccg
     4861 cctgcccttt ccattcgtgc aattacgtcc ggggtgcaaa cggtctggtt ggtaagatcg
     4921 aggcccatca tcactaatgg aacgccggag gtgaacacca cgcgtgcggc ttccgggtcg
     4981 gcaaagatat tgaactcggc agatggggtg aagttgcctg taccgtaagc gccgcccatt
     5041 agcacaattt cacggatttt gggcaggatt gcgggttgca tacgcattgc caccgcgata
     5101 tttgaaagcg gaccaaccgg caccagagtg atatcgccat cgcttgccat aagggtatca
     5161 atgatgtatt tcaccgcgtg ggtactttct gcctggcggg tcagcggctc gaataccggg
     5221 ccatccagtc cggtttcacc gtgaatatta tcggcaacga tttgttgacg cataatgggt
     5281 tgcggcatcc ccgcataaac cggaacatta atctccagtt tctggcaaac attcaggcca
     5341 ttaattaatg ttttatcaag cgtctgatta cccgcaacaa tggtgatacc taataaatca
     5401 atcgccggat gtttcgccgc catcattata gcaatagcat catcatgacc cggatcacaa
     5461 tccagaataa tttttctctt ttccattgtt tatttcctct gtttccagtt gcgttatttt
     5521 ttctacagca acgaaatttc gatcgggccg atgattgaat cttaacaaca gcgtacgaat
     5581 gctaaatatg agaaatctca tagcggataa acatcgtgaa agaaatccac aataatgatc
     5641 ttaagcagca attgatgagt gaatccgcgt ttaaggattg ctttttaacg gatgtttcag
     5701 ccgatacgcg gctgtttcat tttttagcgc gtgactacat tgtgcaggaa gggcaacagc
     5761 cgtcctggct gttttacctg acgcgaggcc gcgccaggct ttacgccacg ctagctaatg
     5821 gtcgcgtgtc gctgatcgat ttctttgccg ccccctgttt tattggcgag attgagttaa
     5881 tcgacaaaga ccatgaaccg cgtgcggtgc aggctattga agagtgttgg tgccttgcgc
     5941 tccctatgaa acattaccgc ccgctgttat taaacgacac gctattttta cgaaaactct
     6001 gcgtcacctt aagtcataaa aattatcgta atattgtttc tttaactcag aatcaatcat
     6061 ttccgttagt gaatcgcctg gcagcattta tattactctc gcaggaaggt gatctttatc
     6121 acgaaaagca tacgcaagcg gcagagtatt taggcgtttc ttatcgacat cttttatatg
     6181 ttctcgcgca attcattcac gacggtttat taattaaaag caagaaaggg tatctaatta
     6241 aaaacagaaa gcagttgtca ggactggcgc tggagatgga cccggagaat aaattctccg
     6301 ggatgatgca gtaaaaatta ttggcaatag cacgattgcc ggatgcaacg cttaataagg
     6361 cgtacatccg gcaatgaaac tcaagctaaa ccaataaaga acccggcaat ggtcgcgctc
     6421 atcaggttgg aaagcgtcgc cgccgccagc gcccgtaaac caagctgggc gatttccggc
     6481 gcgcgttttg gcgaaatagc cgaaaacgct ccaacgacaa caccgataga accaaagttt
     6541 gcaaacccac agagggcgaa cgagataatg gcaatggtct tgacatccag cgtgccgctc
     6601 gtttgcagat agggcgagaa attgagataa gcaacaaact cattaatcgc cagtttctgc
     6661 ccaatcaaac ttccggcaag atttgcatca ctccagtcca cccccataat ccatgccaac
     6721 ggggccagca catagccgaa aatgctttcc agagagacat tggcaaaacc aaaccagccg
     6781 ccaactccac caataatgcc gttgatcagc gcaatgatcg ctacaaatgc cattaccacc
     6841 gtcgccacac cggcggcgat tttcagcccg gtcattgcac cgttcgccgc agcttcaata
     6901 atgctttttg gcggtgtttc gctaaacgac agattttcaa acgtaacttg cgaaggttcc
     6961 gtcgccggac taagaatacg tgcaaacagg ataccgccag gaatagccat cagcgaagcg
     7021 gctaacaaat agtcaatcgg cacgcccatt ccggcatagc caatcatcat cgaacccgca
     7081 atagacgcca tgccgctaca aatagccgta aatagctcgt tgcgattcat gcgatcaata
     7141 aaaggtttaa cgatcgccgg gatctcattt tgcccgagga aaatagtggt gactgcgaca
     7201 aaagattcga ttttgctgat gttgagggct ttctgaaaaa tactgccaag aatacgaatc
     7261 agcagcccca ttacgccaat gtagtacagc aagctgataa gcgcggtaac gaaaataatc
     7321 gccgggagta cgcgaaaggc gaagataaag cccgcaccgt caaacagaac atccattttg
     7381 ggaccgacca gcgaaccaaa aataaaggca cttcctgcgt cactgtagga catcacttta
     7441 tggacgccta atgccgcctg ctctactgcc cattttcccg ggggaaaata gagcatgatg
     7501 ccaccaatgg caatttgcag cagcagcgca gcgccaaccg tgcgcaagct gatgctcttt
     7561 ttatttaccg acaacacaaa ggcaattgcc agcaatacca ccatccccgc aacacttctc
     7621 attatatcca taatgatttt cccttcatgc cagtaaaccc ggcgtcagcg ccaggttttg
     7681 gtatgtttga tgagtacggg cgacggcttt ctgcccgcca gaaattaacc cacgagacgc
     7741 tgatattctt tggcaatttc gctcgccaga atggcgttat taaagaccag ttgaatattg
     7801 gatttcaggc tatcaccgcc ggttagctcc gcaacacgcg ccagcaggaa cggtgtactc
     7861 tctttcccaa taacaccctg ttcttcggct tcagcaacgg cttgatcaat tgctgcattg
     7921 atggtttctt ctggcatcgc aaactgttcc gggatcgggt tcgctaccac caggccgccg
     7981 ttcaaaccgg tttgccactt cactgccatc gcgcgagcaa tttcgcgggc actgtcaaga
     8041 cgaatgctga cttcaaatgg gctggttcgg cagaaaaacg caggcagcgc ttttgtctga
     8101 taaccaatta acggtacacc gaaggtttct aaatattctg ttgttaatcc taaatcgaga
     8161 atagattttg cccccgcaca aacaacagtg acattggtat ttgccagttc ctgtaaatcg
     8221 gcagaaatat cgaaggtatg ttccgctccg cgatgcacac caccaatacc tccggttgca
     8281 aaaactttaa tcccagccag tgctgcaata atcatggttg atgcaacggt agtcgcgcca
     8341 tttttccctg cagcaacgac aaaaggtaaa tcacgacgac taactttcgt cacgttatgt
     8401 ccttcacggc ccagcaattc gatttcttct ttacttaagc ccactttcat cacaccgcca
     8461 ataatggcga tagtggctgg tactgcgccc tgtttacgaa ttgtttcttc tacttcaatt
     8521 gcggtttggg cattttgtgg gaatggcatt ccgtgagaaa ttatggtcga ttccagcgca
     8581 acaaccggtt ttttattttt taaagcatcc tgtacttcag cggatatttg taataattca
     8641 ggggacagag ttaattcaga cattctgtgt tctccactaa cgatctaaca ttggcaattg
     8701 ataattcagg gttgttggta tattcacagg caagtgccat tgacgaacat ccctgcgcaa
     8761 atcgaacaga atcgataaac ggcataccgt ctacccaaca ggaggccagc cccgccatca
     8821 tggcgtcgcc tgccccagta acattgatga cattcgtttt aataggggcc gaccacccac
     8881 tttcaccgtt gatatcactg tagtaaacgc catcccctcc catgctcagc accaagcggt
     8941 tcaggccgtg ttgatgaaac caggcggcga cctttgcgac atcttcacgt ccggaaagtg
     9001 caattccgct caacgtttcg gcttcaagac ggttaggttt taacgtatgg attttgctca
     9061 gatgatcgcg gattttgacg catttccatg ccgataccgg atcaataaat acaggcgttt
     9121 ccccgctatt ttccagaacc cagaccagcg cctcttcact aaggttgcaa tccgcgacaa
     9181 taacctttgc accgcgaata aactcctgat gctgggcaag aaattctgca gaaatacagt
     9241 cactaatact catatcattt atcgcgacaa gcatttctcc tgtattatcg agcagtgata
     9301 aatagctgga tgtattctca cccggaacaa ttaagcattt atcgacataa acaccggatt
     9361 gattagtttg cgccagtaat gattgcccat aaaaatcacc acctactgca cttaaaagcc
     9421 aggcattttt ccccagtaac gccagattat gagcgatatt ccttcccacg ccacctgggg
     9481 taaatttaat ttttccggga ttcgaatccg cataattcaa tggatgatgt gaatacccag
     9541 ccacatcaat attcgccgaa ccaataatta ctatgtagtc cttttcgcgc atagcacgcc
     9601 ccctggcaat aaattgccaa cgaagaaatt gggtaaaata gccattacag tcaataagac
     9661 tattagagca cgtgttcaga tttaaacaca tgttcataat aagcatattt aacgtaatgt
     9721 aaatcgatca gaaaaaacga aatcggccag atatgaaggc ggtcacaaag tttgtctatt
     9781 tatgcagcaa caaaagagaa agttgtgatg tgtaacacac aatatggcag gcaagaaaaa
     9841 gggcagagat gtttctgccc tccagattgt tgacaacgcg cgctttgttc atcctggatg
     9901 tggcgtaaac gccttatccg acctacaaaa gcgcaaaaaa ttcaatatat tgcagggttc
     9961 atgtaggtct gataagcata gcgcatcagg caattttgcg tttgtcatca tcggggcaga
    10021 gatgtctcat ccccgataat gaagataaat tatgcgtttt tttctactat ttgtgtttcc
    10081 gggcgtttca ggaaggcata ggccaaaccc gccaccagcg taccggcaat aattgctacc
    10141 aggtaaccca ataccggcgt aatagcgcca gggatcagca gaacaaacag accaccgtgt
    10201 ggtgccatca gtttcgcacc aatcgccatt gagattgcgc cagtcagcgc cccaccaacg
    10261 atacagcacg gtagcacacg catcggatca cgagcagcaa acggaattgc accttccgaa
    10321 ataaagcaca gaccaagtac cagagcggct ttgccacctt cctgctgcgc tttgtcgaat
    10381 ttgcgacgcg ccaccattgt tgccagaccc attgccagcg gtggcaccat acctgccgcc
    10441 ataatcgccg ccatcgggcc ataggtttga gtactcagca gacccacacc gaatgcgtac
    10501 gctgctttgt ttaccggacc gcccatgtca gtacacatca tgccaccgag gatcgccccc
    10561 agcagaaccg cattcgcagt ccccatggtc tgcagccagt gagtcaaccc atcgagaatg
    10621 ccagcaaccg gtttaccgat taggtagatc atcgccagac cgaccaccag actggaaatt
    10681 agcggaatga tcaggatcgg tttcagcgcc tccatactct gtggcagttt caattgcgta
    10741 ctgattaact tcgcaatgta accagccagg aagccagcaa ttataccgcc aataaagcca
    10801 gaaccggtgc tgaccgccag cataccgcca atcagacccg gtgtgaggcc cggacgatcg
    10861 gcaatggaga aggcaatata acctgccagt accggcacca tcagcgcaaa ggctgaacca
    10921 ccaccaatct gcatcagcgc cgctgccaac gtacccggct ctttaaacgc ttcgatacca
    10981 aaagcaaaag aaagcgcgat acacagacca cctgcaacaa ccatcggcag catgtaagag
    11041 acgcccgtca gcaagtgacg gtaagcgcct gcactctctt tcttaccttc agtggtcgct
    11101 gtttgagctt tgcccgctgg ttcatacggc gttgcttcag caaccgcttt atccagttcc
    11161 tgcgcagttt tcttcagcgc cagaccggta gaggtgcgat acatcggttt accagcaaat
    11221 ttcgccagat ccacttcgat atctgccgcc acaatcacca gatccgctgc tgcgacttct
    11281 tcaggggtga ttgcattgcc cgcgccaaca gaaccacggg tttcaacttt cacccaccag
    11341 ccacgttttt tcgcttcggt ttcaatggct tcagccgcca taaaggtgtg tgctacgcca
    11401 gtcgggcaag cagtcaccgc aactacgcgt ttcggaccgc tggcagcaac cggtgctgtc
    11461 gcagcgaccg gcgcagtgta aggtttcgca tggcctttgg cttcactcag gaacagctca
    11521 gggtgcgcaa ctgcccggga aatatcaccc agccagacat ttttaccgtt cagcgcgctg
    11581 tcattcggga tggaatcacc gagaacaatc gccatttctg cgtcgttcgg attgtcgatg
    11641 atttccagtt ttgcttttcg cgccgccgcg cccagcaggg tcttcgccat ataggcgcgt
    11701 gcctgaccga gattagcgtc aataatcagc agcgttttca ttatgcctct cctgctgtca
    11761 gttaaaaggt tgtaagtcga cgcgcgccat cattgcggcc aactgcggac gatcggtaat
    11821 acccacattg ctttgactta ccgccagggc tgcaacagct gtcgccagac gcagtgtgtg
    11881 ttcactggat tcacgcatca gcaagccata aatcaggcca ccaaccatag aatcccctgc
    11941 gccaacggtg cttacgacat cgactgacgg tggtttggcg atccattcgc cggaggcatt
    12001 aacccaaagc gcgccttcgg cacccagtga aataacaaca tgcgcaatac cttgttcacg
    12061 tagcgcatgt gcagcttcaa tcacatcttt catttcaggc agtttacggc ctgcccagat
    12121 ttccaactcg cggcggttag gtttcaccag ccacggtgcc gctttcaaac ctgctactaa
    12181 cgcttcacgg ctactatcaa agataatgca aggacactga ctacgcaggc gagtcatcca
    12241 gtcggtgaac gcttccgggc tgacgcctga cggtaagctt ccgctgacac agaccatatc
    12301 gaactgaccg agccagctca gagaatcagt cacaaagcgt tcccagtcgg cgagggtgac
    12361 ttcaaaaccc gagaagttga agtcggtcac ttcgccgtct ttttccgtca gcttaacgtt
    12421 aattcgggtg cgcccctgta caacctggaa acggttggca atgcccagct cgctgaacag
    12481 ttgctgaaaa ccatcctgat tgtctttacc caggaagccg ccaacggtga catcaattcc
    12541 caggtctttt aatactttgg ccacgttgat gcctttaccc gccgcatgca gaccggtggt
    12601 tttcaccagg ttcacttcgc cgcgttcaat ttccgggcag aaaccaacaa ggtcataagc
    12661 cggattaagg gtgatcgtag caacacgtct gctcattatg cgccctcccc aagaccagca
    12721 gcgatagcgt cgccaatggc tttcagcgcc tgttcagcat ctgcaccctg ggcggtaaag
    12781 cgtaggcgat gacctttctt aacgccaagt gccacaactt tcatcagact acgtccgttt
    12841 gccggtttgc cggtgccatc aaggtttgtc acggtaatat cactgttaaa ttgtttaatg
    12901 gtattgacca gcatggtacc tggacgagca tgcaggccgt gttcattgcg caccacaaac
    12961 tccgcgctta acacgtcgtc ggtcggcgca tcatcgctgg tcagcagcgc cagcaacgtt
    13021 gccgcatccg ctttcagcaa gcggtcagct ttattgtcga gcaataaatc agcgagacgc
    13081 ttaagaaccg cgatgggctg atcgtcattc atcgccacac tcaccagcat ggctgccgtt
    13141 tcgccgtcca catcaaaagc atttgccgca cggcttaccg caatcgcgct acgcagattg
    13201 ccttcggcgc tatcgctcag ccagataccc tgtccgagat tcagcggttg ttcattgatg
    13261 gctttggtga cgaaagtggc gtcaactgcc cccacctctt tcagacgcgc agcgttcagc
    13321 gcctgaagag tcagcagatc gctggcgacg atatccagcg tcagcatttc gttgtcgagc
    13381 ttcagctgct cactctgctt ttcgcccatc agtaatgcgc gaagttcttc tgctgttgtt
    13441 gctgacttca gttgttcagc aacggaatca tcgctcagta cgtgggtcag ctggcgtagc
    13501 aggcccagat gttcatccga gctggcagca ataccgattg ccacgtacgc tacctgaccg
    13561 tcaccccagg tgacgccttc cgggaactga aatacctgaa cgccggtttt cagcacctga
    13621 tcgcgggtgt cggtagtgcc gtgtggaata gcaataccat tgccgaggaa cgttgaggtt
    13681 tgctgttcgc gcgccagcat gccattgacg tagccttctg ctacattacc ggcctgcacc
    13741 agcgccgcag cgacctggcg aatcgcctct tctttgtctc cggccttttc gcccggatgg
    13801 atgtcctgta cggataactg gaacatagtt ctcctctctt gctgaattga aacgattcag
    13861 cctctatgag aaaaaaagcg ccaacctggc ttagggttaa agacaagatc gcgctgaaac
    13921 gtttcaagaa agcataatac ttctgtttca gcacgcaagg aaagccgtaa agttatgagc
    13981 aaaagtttga tgtgctgcac aattttttgt catttttcct aattgctgat gggaaaatcc
    14041 gttatcagca atttcatttc agcagcgtca gcagccaatc agcaacagtg aaatggcatt
    14101 ttgattattc gtggcgcttt tgatttcgct gtctgtttat tttctgacaa gcagcgtaaa
    14161 ctccgcgtct tcctcttcca gtgatcgacc tgcatgcata actcccccgc agtctccagc
    14221 gcgaaatcgt ttgacctgac ctcgacggcg tttttaatcg ttgcctttct caccggtatt
    14281 gcgggtgctc tgcaaacccc gacactcagt attttcctta ccgatgaagt acatgcccgt
    14341 ccggcgatgg tgggattctt ctttaccggc agcgctgtca ttgggattct ggtaagtcag
    14401 tttctcgctg ggcgctctga taagcgcggc gatcgcaaat cgctgattgt cttttgctgc
    14461 ctgttaggcg tgctggcctg cacccttttt gcctggaatc gcaactactt tgttttgcta
    14521 ttcgttggcg tctttcttag cagctttggc tcgaccgcta acccgcaaat gtttgccctt
    14581 gcccgtgaac atgccgacaa aaccggacgt gaggcggtga tgttcagctc ttttttacgc
    14641 gctcaggttt cactggcatg ggtcattggc ccaccgctgg cttatgcgtt agcgatgggt
    14701 ttcagcttta cggtaatgta tctgagcgca gcggtagcgt ttattgtttg cggcgtgatg
    14761 gtgtggctgt ttttaccgtc gatgcaaaaa gagcttccgc tggcgaccgg cacggttgaa
    14821 gcgccgcgcc gtaaccgtcg cgatacgcta ctgctgtttg tcatttgtac attgatgtgg
    14881 ggctcgaaca gcctgtacat catcaacatg ccgctattta ttatcaacga actgcatctt
    14941 cccgagaaac tggccgatgt gatgatgggg accgccgccg ggctggaaat cccgacgatg
    15001 ttgattgccg gatatttcgc caaacgtctg ggtaagcgtt tcttaatgcg cgttgctgcc
    15061 gtgggtggcg tctgttttta cgcaggaatg ctgatggcgc attcacctgt cattctgttg
    15121 ggcttgcagc tgctaaatgc tatttttatt ggcattctgg gcggcatcgg gatgctctat
    15181 tttcaggatc tgatgcccgg tcaggcgggt tcagccacca cgctctatac caacacttcg
    15241 cgcgtgggct ggatcatcgc aggatcagtg gcgggcatcg tcgccgaaat ctggaattat
    15301 cacgctgtgt tctggtttgc gatggtgatg attatcgcca ctctgttttg cttactgcgg
    15361 attaaagatg tttaaggcgc ggtcagcatt tccagatcga taagccaggt catggcctgc
    15421 tgacgcgagt taccgcacat ttcagcgctg gcttgtaagc ctgcacagac tttcggtcgc
    15481 agtggcgagg tgaaaatttt acagcgttgc tgttcatcaa gctgaatgca gggcgtattg
    15541 gcgggcttgc catctggcat accgggaata gggctggaaa ttgaaggggc agtgcaacac
    15601 gccccgcaac ccggacggca ttccatacgt tgttctctta aggttaacca ccggattgat
    15661 gagcgcgcac agtaccacct tttttgcacc agcaaaagtg cgaataccac ttgcccgaaa
    15721 ggcccgtcgc gagtactttg tcgcgatatt tttgacattt tcgactacag gaatttttcg
    15781 atgccaagag cgaacgaaat taaaaaaggt atggtactga attacaacgg caaactgctg
    15841 ctggttaagg atattgatat tcagtcgccc actgcccgcg gtgccgctac gctgtacaaa
    15901 atgcgttttt ctgatgtccg taccgggctg aaagtagaag agcgtttcaa aggtgatgat
    15961 atcgttgaca ccgtgacgct gacccgccgt tacgttgact tctcctatgt cgatggcaac
    16021 gaatatgtct ttatggataa agaagactat accccgtata ccttcaccaa agatcagatt
    16081 gaagaagagt tgctgtttat gccagagggc ggcatgccgg acatgcaggt gctgacctgg
    16141 gacggtcaac tgctggcgct tgagctgccg cagaccgttg atctggaaat cgttgaaacc
    16201 gcaccaggca tcaaaggggc atctgccagc gcccgtaaca aaccggcgac attgagcact
    16261 ggtctggtga ttcaggtacc agaatactta agcccgggcg aaaaaattcg tatccatatc
    16321 gaagaacgcc gttatatggg gcgtgctgac taacttcagc cgcatgcaga aaagggatag
    16381 ctcaggctgt ccctttttta atttattata ccaatcttct attttgcgct tcctgaacac
    16441 cttacgccct caattttcac tcgttgattg atcgccctca cacttcatcg cattaacaat
    16501 ccagaccaat ttcaattgct gtcatataac tttacactgt cattgttaat ttatcgttac
    16561 taagacgtga ctcctatgaa gacaattgcc tccgttacgc tcccgcatca tgtacacgct
    16621 ccacgctacg atcgccagca gttgcaatca cgtatcgttc attttggctt tggcgccttt
    16681 caccgcgctc atcaggcgtt actgaccgat cgtgtgctga atgcccaggg cggcgactgg
    16741 gggatctgtg aaatcagctt gttcagcggt gatcaactga tgagccagct ccgcgcacag
    16801 aaccatttat ataccgtgct ggagaaaggt gcggacggca atcaggtgat aattgtcggt
    16861 gccgttcacg aatgccttaa tgcaaaactg gattccttag cggcaattat tgagaaattt
    16921 tgcgagccac aggtggcaat tgtttccctg acgattaccg aaaaaggcta ttgtattgac
    16981 ccggccaccg gtgcactcga caccagtaat ccgcggatta ttcacgatct acaaacccct
    17041 gaagaacctc actccgcacc gggtattctc gtcgaagcac tgaaacgccg ccgtgagcgc
    17101 ggccttacac cgtttaccgt gctctcctgc gacaatattc ccgacaatgg ccatgtggtg
    17161 aaaaacgcgg tgctgggaat ggcagaaaaa tgttcgccag aactcgccgg gtggataaaa
    17221 gaacacgtca gttttccggg aaccatggtc gaccgcattg ttccggctgc aaccgacgaa
    17281 tcactggcag aaatcagcca gcatctgggg gtaaatgatc cctgcgcgat tagctgcgaa
    17341 ccgtttatcc agtgggtggt tgaagataac ttcatcgctg ggcgtcctgc ctgggaagtc
    17401 gcaggtgtac aaatggtgaa tgatgtcctg ccatgggaag agatgaaact gcgtatgctt
    17461 aatggcagcc actcttttct cgcttatctg ggttacctct caggattcgt ccatatcagt
    17521 gattgcatgc aggatcgcgt atttcgccat gctgccagaa cattaatgct ggatgagcaa
    17581 gcgccgacac tgcaaattaa agatgtcgat ttaacacaat atgcggataa gttaattgca
    17641 cgttttgcta atccgtcgct gaaacataag acctggcaaa tcgcgatgga tggcagccag
    17701 aaattaccgc aacgtatgct ggcaggtatt cgcatacatc tggggcgtga aacggactgg
    17761 tcgttgctgg cattaggcgt tgctggctgg atgcgttacg tcagcggcgt tgatgatgcc
    17821 ggaaatgcca ttgatgttcg cgatccgctt agcgataaaa ttcgcgaact tgttgcgggc
    17881 agcagcagtg aacaacgcgt aaccgccctg ctttccctgc gtgaagtttt cggtgatgat
    17941 ctgccagata acccgcattt tgtgcaggcc atcgaacaag cctggcaaca aatcgtacaa
    18001 ttcggcgcac atcaggcgct attaaacacc ctcaaaattt aacgatttct gcggttaaag
    18061 cggatgaagc tcaccttcgt ccgctctccc cttctctttt ctgccttttt tagccaggat
    18121 taacgctcag ttaacttacc agagtattgc ggagccgttg tgaccaggac caacctcatc
    18181 accggttttc tcggcagcgg gaaaaccacg tcgattcttc atctgttagc ccataaagat
    18241 cccaacgaaa aatgggcggt actggttaat gaatttgggg aagtcggaat tgatggtgct
    18301 ttgctcgccg atagcggcgc attgctgaaa gagatccccg gcggctgcat gtgctgcgtt
    18361 aatggtttac ccatgcaggt agggttgaat accttactgc gtcagggaaa accagaccgc
    18421 ttgttgatag agccgaccgg gctgggccat ccgaaacaga tcctcgatct gttaaccgca
    18481 ccagtctatg aactgtggat agatctgcgc gccaccttgt gcattctcga tccacgcctg
    18541 ctgctggacg aaaaaagcgc cagcaatgaa aacttccgtg accagctggc tgccgcagac
    18601 atcattgtcg ccaataaatc cgaccgtgcg acgcccgaaa gtgagcaagc gctacagcgt
    18661 tggtggcagc aaaatggtgg cgatcgacaa ttaattcaca gtgagcatgg gaaagttgac
    18721 ggtcatcttc tggatttgcc gcgtcgcaat ttagccgagt tgcccgccag cgccgcgcat
    18781 tctcatcagc atgtcgtgaa aaaagggtta gcagcgttaa gcctgccaga gcatcaacgc
    18841 tggcgtcgca gtctgaacag cgggcaagga tatcaggcct gcggctggat attcgacgct
    18901 gatacggtat tcgacaccat tggcattctg gaatgggcgc gacttgcacc ggtggaacgc
    18961 gtcaaaggcg tgctgcgtat tcccgaaggg ctggtacgaa tcaaccgtca gggcgatgac
    19021 ctgcacattg aaacgcaaaa cgttgcgcca ccggacagcc gtattgagct gatttccagc
    19081 agcgaagctg actggaatgc tttgcagagc gcgctgttga agcttcgttt agcgactacc
    19141 gcgtaaggtt gcctgcgttt ttcagtaaga taattagaga aaatatgatt aaaaatttgc
    19201 cgcaaatagt gttgttgaat attgtcggcc tcgcgctgtt tctttcctgg tatattcccg
    19261 ttaatcatgg attctggttg ccgattgatg cggatatttt ttatttcttt aatcagaaac
    19321 tggtcgaaag taaggccttt ttgtggctgg ttgcattgac caacaatcgc gctttcgacg
    19381 gttgttctct gctggcgatg ggaatgttga tgctgagttt ctggctgaaa gaaaacgccc
    19441 ctggcagacg acgtatcgtg attatgggtc tggtcatgct attaactgca gtggtattaa
    19501 accagctggg tcaggcatta attcctgtaa aacgggccag cccaacattg acttttaccg
    19561 atattaaccg cgtcagcgaa ctgctctctg ttcccacgaa agatgcctca cgagatagct
    19621 ttcccggcga tcacggcatg atgctgctta ttttttcggc attcatgtgg cgttatttcg
    19681 gcaaagttgc aggccttatc gcccttatta tttttgtggt ttttgcattt cccagagtaa
    19741 tgattggcgc acactggttt actgacatca ttgtcggttc gatgaccgtg atattgatcg
    19801 gtttgccctg ggtgttgctg acgccattaa gtgatcgatt aatcaccttt tttgacaaat
    19861 cactaccggg aaaaaacaaa catttccaaa acaaataact cacagtaatt aacatcatca
    19921 ggcttatttt tatagtgagg ataatcctga tgatgcgcac cgtgctttca tccatcgcgc
    19981 gcaaaaatca ttctctaagt aaatgaatgg attgcatgcg tttcactcaa tcgtacttta
    20041 attgaccaat cccgcttatt aactttctgt atcacttttt cttataaaaa atcatgtaaa
    20101 accgctcgcc aagaccgcac caatcgggta atctcgaact cgttttgcct cggcggtaga
    20161 ttatcctcac agcatataat tttgtgcgtt agtccacaga tttggcctta aggaattgtt
    20221 tcaacatgcc caggtaatta gtctcgtgcc gcttggcatt tttttataac gatatttgtc
    20281 gttaaggact tcaagggaaa acaaacaaca tggtcaaatc tcaaccgatt ttgagatata
    20341 tcttgcgcgg gatccccgcg attgcagtag cggttctgct ttctgcatgt agtgcaaata
    20401 acaccgcaaa gaatatgcat cctgagacac gtgcagtggg tagtgaaaca tcatcactgc
    20461 aagcttctca ggatgaattt gaaaacctgg ttcgtaatgt cgacgtaaaa tcgcgaatta
    20521 tggatcagta tgctgactgg aaaggcgtac gttatcgtct gggcggcagc actaaaaaag
    20581 gtatcgattg ttctggtttt gtacaacgta cattccgtga gcaatttggc ttagaacttc
    20641 cgcgttcgac ttacgaacag caggaaatgg gtaaatctgt ttcccgcagt aatttgcgta
    20701 cgggtgattt agttctgttc cgtgccggtt caacgggacg ccatgtcggt atttatatcg
    20761 gcaacaatca gtttgtccat gcttccacca gcagtggtgt tattatttcc agcatgaatg
    20821 aaccgtactg gaagaagcgt tacaacgaag cacgccgggt tctcagccgc agctaataaa
    20881 ccgtttggat gcaatccctt ggctatcctg acgagttaac tgaaagcact gcttaggcag
    20941 tgcttttttg ttttcattca tcagagaaaa tgatgtttcc gcgtcttgat ccaggctata
    21001 gtccggtcat tgttatcttt taaatgttgt cgtaatttca ggaaattaac ggaatcatgt
    21061 tcatacgcgc tcccaattct ggacgtaagc tcctgcttac ctgcattgta gcaggcgtga
    21121 tgattgcgat actggttagc tgccttcagt ttttagtggc ctggcataag cacgaagtca
    21181 aatacgacac actgattatc gacgtacaaa agtatctcga tacctatttt gccgacctga
    21241 aatccactac tgaccggctt cagccgctga ccttagatac ctgccagcag gctaaccccg
    21301 aactgaccgc tcgcgcggcg tttagcatga atgtccgtac gtttgtgctg gtgaaagata
    21361 aaaaaacatt ctgttcatct gcgactggtg agatggacat tccactcaat gaattgattc
    21421 cggcgctcga cattaataaa aacgtcgata tggcgatctt acccggcacg ccgatggtgc
    21481 cgaacaaacc cgcaatcgtc atctggtatc gcaacccttt gctgaaaaat agcggtgtgt
    21541 ttgccgctct gaatctcaac ctgacgcctt ctctctttta tagttcacgg caggaagatt
    21601 acgatggcgt cgccctcatt attggcaata ctgcgctatc taccttttct tcacgtttga
    21661 tgaacgttaa tgaattaacc gacatgccgg tccgtgaaac taaaattgcg ggcattcctc
    21721 tgaccgttcg gctttatgcg gatgactgga catggaacga tgtgtggtac gcatttttac
    21781 tgggcggcat gagtggaact gtcgttggcc tgctctgcta ttacctgatg agcgtacgta
    21841 tgcgccccgg cagagaaatc atgaccgcca tcaagcgcga acaattttac gtggcgtatc
    21901 aaccggtggt ggatacacaa gctttgcgag taacgggcct ggaagtactg ctacgctggc
    21961 ggcatcctgt cgcgggagaa attcccccgg atgccttcat taactttgcc gaatcgcaaa
    22021 agatgattgt gccgctgact cagcacctgt ttgagttaat tgcccgcgat gccgcagaat
    22081 tagaaaaagt gctgccagta ggcgtcaaat ttggtattaa cattgcgccg gaccatctgc
    22141 acagcgaaag ctttaaagca gatatccaga aactgctcac ttccctgccc gcacaccatt
    22201 tccagattgt gctggaaatt accgagcgcg atatgctgaa agagcaagaa gccacacaac
    22261 tcttcgcctg gctgcactcg gtcggcgtag aaattgctat tgatgacttc ggcaccgggc
    22321 acagcgcgct tatctatctt gagcgtttta cgctcgatta tctgaaaatt gaccgtggat
    22381 ttatcaacgc catcggtacg gaaacgatca cttcacccgt acttgacgcg gtgctgacgc
    22441 tggcgaaacg cctcaatatg ctgacggttg ctgaaggggt cgaaacgccg gaacaggcgc
    22501 gatggctaag cgaacgcggc gttaatttca tgcaaggcta ctggattagt cgccctttac
    22561 cgctggacga ttttgtccgc tggctgaaga aaccgtatac gccgcagtgg taaggtgtgc
    22621 ttacgtccct tattattcat agtgaaagca tgccggattg cggctaatga tgagtaaaag
    22681 gaaatccatt gcagatgatt gtgcgcatac tgctgctgtt tatcgctctg ttcacctttg
    22741 gtgtgcaggc gcaggctatc aaggaaagct atgcctttgc cgtgctgggc gaaccccggt
    22801 acgcgtttaa tttcaaccat tttgattatg tgaaccccgc cgcgccaaaa ggtgggcaga
    22861 taacgttgtc agccctcggc accttcgata atttcaaccg ctatgcactg cgcggcaacc
    22921 cgggcgcacg caccgagcag ctgtacgaca cgctatttac gacttccgat gacgaaccag
    22981 gcagttatta cccgctgatt gctgaaagcg cacgctatgc tgacgattat tcctgggtgg
    23041 aggtcgctat taatccgcgc gcccgttttc atgatggttc gcccattact gcccgcgatg
    23101 tagagtttac ttttcaaaaa tttatgaccg aaggcgtgcc gcaatttcgt ctggtctaca
    23161 aaggcaccac cgtcaaagcc attgcaccgt taaccgtgcg cattgagtta gctaaaccca
    23221 gcaaagaaga tatgctgagt ctgttttcgc tgccgatatt tccagaaaag tactggaagg
    23281 atcacaaact tagcgacccg ctcgccacgc ctccgcttgc cagtggtccg taccgcgtta
    23341 cgtcctggaa aatggggcaa aatattgtct attcccgcgt gaaagattac tgggcagcaa
    23401 acttaccggt aaaccgtgga cgctggaatt tcgacaccat tcgctacgat tattacctcg
    23461 atgataatgt cgcctttgaa gcgtttaaag caggtgcctt tgatttgcgt atggaaaacg
    23521 acgctaaaaa ctgggccacg cgttataccg gtaaaaattt cgataaaaaa tacatcatca
    23581 aagatgagca aaagaacgaa tcagcccagg atacgcgctg gctggcgttt aatatccaac
    23641 gtccggtatt cagcgatcgc cgggtccggg aagcaatcac tctcgccttt gactttgaat
    23701 ggatgaacaa ggcgttgttt tacaacgcct ggagtcgcac gaacagttat tttcagaata
    23761 ccgaatacgc ggccagaaat taccccgacg ccgcggagct ggtgcttctg gcaccaatga
    23821 aaaaagatct accgccagaa gtcttcacac aaatctacca gccgccggta tctaaaggcg
    23881 atggctacga tcgtgacaac ctgttaaaag ccgacaaact tctcaacgaa acgggctggg
    23941 tgctgaaggg tcagcaacgc gttaatgcca caacgggtca gccgctcagc tttgaattat
    24001 tgcttcccgc aagcagcaat agtcagtggg tattgccgtt ccagcacagc ctgcaacggc
    24061 tgggtatcaa tatggacatt cgcaaggtgg ataactcgca aatcactaac cgcatgcgca
    24121 gtcgcgacta tgacatgatg ccgcgcctat ggcgggcgat gccgtggccc agttccgatt
    24181 tacagatttc ctggtcatcg gaatatatca attccactta taatgccccc ggcgtgcaaa
    24241 gcccggttat cgactcgctg atcaaccaaa ttattgccgc gcagggaaat aaagaaaaat
    24301 tactgccgtt ggggcgagca ctggatcgcg tattaacgtg gaattattac atgctgccaa
    24361 tgtggtacat ggcggaagac cgtctcgcct ggtgggataa attctcccaa cccgctgtac
    24421 gccctgttta cagcctgggt atcgatacct ggtggtatga cgttaataaa gcggccaaac
    24481 tgccgtcagc cagacaacag ggagagtaga tgggcgctta cctgattcgc cgtctgttgc
    24541 tggtgatccc aacgttatgg gctattatca ctatcaactt tttcatcgtg caaattgcgc
    24601 ctggcggtcc ggtcgatcag gccatcgccg ccattgagtt tggtaatgcc ggagtattac
    24661 ccggcgcagg cggtgaaggt gttcgtgcca gccatgcgca aacgggtgtc ggcaatatca
    24721 gcgacagtaa ttaccgtggc ggacgcggat tagacccaga agtgatcgct gagatcactc
    24781 atcgctacgg tttcgataag ccgatccacg aacgttactt caaaatgctc tgggactaca
    24841 tccgctttga ttttggtgat agcctgtttc gcagcgcctc ggtgctgacg ctgattaaag
    24901 acagtctgcc ggtttccatc accctcggat tgtggagcac gctgattatc tatctggtgt
    24961 cgattccgtt aggcattcgc aaagctgttt ataatgggag tcgctttgac gtctggagta
    25021 gcgcatttat catcatcggc tacgccattc cggccttttt gtttgccatc ctgctgattg
    25081 tcttcttcgc gggcggcagc tatttcgacc tgttccctct acgcggcctg gtttccgcta
    25141 actttgattc gctgccgtgg tatcagaaaa tcaccgatta tctgtggcat atcacgctac
    25201 cggtgctggc gacagtgatt ggtggctttg cggcgctgac catgctgacc aaaaactcat
    25261 tccttgatga agtgcgcaag caatacgtgg tgaccgcgcg tgcgaaaggg gtaagtgaga
    25321 aaaacattct ctggaaacat gtgttccgca acgccatgct gctggtgatt gccggttttc
    25381 cggcgacgtt tatcagcatg ttttttaccg gctcgctgct gattgaggtg atgttttcac
    25441 tcaatggtct tggcttactg ggctacgaag cgaccgtctc gcgcgattat cctgtaatgt
    25501 ttggtacctt gtatattttc accctgattg gcctgctgct gaatattgtc agtgatatca
    25561 gctatacgct ggttgatccg cgtatagatt ttgagggacg ttaatgtcgc gactcagccc
    25621 cgtcaatcag gcccgttggg cgcgttttcg tcataaccgt cgcggctact ggtcgttatg
    25681 gattttcctc gttttgtttg gtttgagttt gtgttctgaa cttatcgcta acgataaacc
    25741 gttgctggtg cgttatgacg gcagttggta tttcccgttg ttgaaaaact acagcgaaag
    25801 cgattttggc ggcccgctgg caagtcaggc tgattatcag gacccgtggc tgaaacaacg
    25861 gctggaaaat aacggctggg tactgtgggc accgattcgc tttggtgcta ccagtatcaa
    25921 ctttgctacc gataagccct tcccttctcc accctcccgg caaaactggc tgggaacgga
    25981 tgccaacggc ggcgatgtgc tggcacgcat tctctatggc acgcggatct cggttctgtt
    26041 tggcctgatg ctgactctct gttccagcgt gatgggcgtg ctggcggggg cgctacaagg
    26101 ctattacggc ggtaaagtcg atctctgggg acaacgcttt attgaagtat ggtcggggat
    26161 gccgacgctg tttttgatta ttttactttc cagcgtcgta cagcctaact tctggtggct
    26221 gctggcaatt actgtcttgt ttggctggat gagtctggtc ggcgtggtgc gggcggagtt
    26281 tttacgtact cgtaatttcg actacattcg tgcggcacag gcgcttggcg tcagcgatcg
    26341 cagtatcatc ctgcgtcata tgttgcctaa tgccatggtc gcgaccctca cctttttacc
    26401 gtttatttta tgtagttcga taaccaccct gacctcgctc gatttcctcg gcttcggtct
    26461 accgctcggt tcaccgtcac tcggtgaact gctgttacaa gggaaaaata accttcaggc
    26521 cccgtggctt gggatcaccg ccttcttgtc ggtggcgata ttattgtctt tgctgatctt
    26581 tattggtgaa gccgtccgcg acgcatttga tcctaataag gcggtgtagc atgacgcaaa
    26641 ctctgttagc gattgaaaat ttgtcggtgg gttttcgcca tcagcaaacc gtacgtacag
    26701 tagtcaatga tgtttcacta cagattgagg ctggcgaaac gctggcgctg gtgggtgagt
    26761 caggttcagg caaaagcgtt accgcgctgt caattttacg cctgctccct tccccgccgg
    26821 ttgaatatct ctccggcgat attcgttttc atggcgaatc gctgcttcat gccagcgatc
    26881 aaacgttgcg cggtgtacgc ggtaataaga tcgccatgat ttttcaggaa ccgatggtgt
    26941 cgttaaatcc attgcatacc ctggaaaaac agctttatga agtgctttca ctccaccgcg
    27001 ggatgcgtcg ggaagcggct cgtggcgaaa ttcttaactg ccttgatcgc gtcggtatcc
    27061 gccaggcggc aaaacggctg acagattatc cgcatcagct ctccggcggc gaacgccagc
    27121 gggtgatgat tgcgatggcg ctgttaacgc gaccggaatt attaattgcc gatgaaccga
    27181 ccaccgcact ggacgtctct gtccaggcgc agattttaca gctgttgcgc gaactgcaag
    27241 gcgagctgaa tatgggcatg ctgtttatta ctcataacct cagcattgtc agaaaactgg
    27301 cccaccgcgt ggcggtaatg caaaacggtc gctgtgtcga gcaaaataac gccgctacgc
    27361 tatttgcctc acccactcat ccttacacac aaaagctact caacagtgaa ccgtcaggcg
    27421 atccggtacc gttgccagaa cctgcctcaa cgttgctgga tgttgaacag cttcaggttg
    27481 ccttccccat tcgcaaaggg attttgaagc gcattgtgga tcataatgtg gtggtgaaaa
    27541 acatcagttt tacgctacgg gcgggtgaaa cactgggttt agtgggcgag tctggttccg
    27601 ggaaaagcac gacgggactg gcgctgctgc gactgattac ctctcagggc agcatcgtct
    27661 ttgacggtca gccactgcaa gatttaaatc gccgccagct gttacctact cgtcatcgta
    27721 ttcaggtggt atttcaggat ccaaactcct cgctcaaccc acgactcaac gttttgcaga
    27781 ttattgagga aggcttacgg gttcaccagc cgacgctttc tgccgcacaa cgcgaacaac
    27841 aagtgatagc cgtgatgcat gaagtgggat tagatcctga aacacgccac cgttatccgg
    27901 cggagttctc tggtggtcag cgacaacgta ttgcgattgc cagggcatta attcttaagc
    27961 cctcactgat catacttgat gaaccgacat catcactcga caaaacggta caggcgcaaa
    28021 tattgacgct attgaaatca ttgcaacaaa agcatcaact ggcctatttg tttatcagcc
    28081 acgatttgca cgttgtccgc gcgttatgtc atcaggttat cgtactgcga caaggggaag
    28141 tagtggaaca aggaccgtgc gcgcgcgtgt ttgccgcacc gcagcaggag tatacgcgtc
    28201 agctactggc gttgagctga cgcttaaaaa ggattgtagt ttgaaaaggg ttcggcaatt
    28261 gccacaccaa aatttttcaa tcggcatgtt gcagcaaact cgtcctggcg atttacaaac
    28321 aggcacggct caccttcaca ctccagcacc gaacatggaa cttcgatgtc gcttaacgcc
    28381 tggcttagtt tatacatcac cgaccatgca ttatcaccat ccgggctaag caatttaagc
    28441 gccaccaggc tgttgtcacc gcacggtcca ttttgcggaa tcggttcaac cttcgaacct
    28501 gcgaaaccag cagaccagcg aaagttctgc ggtagacgat ggacgattga gagttgtaat
    28561 gaagtcactt attttccccg gaagcacatt tacttcacaa tttatttaca tcaattttaa
    28621 cacatcatca acaatccgtc tttatacaga tcgtaaagat tgatgcctcg cctgcatggc
    28681 ctctatggct ctgatttaag taatatctgc gggcgtggtc acgtttgcgg ggctatatgt
    28741 acccgtttct gcgatctaac tcaacctttt taactacaat gatgtgactt tttacataaa
    28801 ttgattttac ataaaataaa catatatcgg gggaatgata gatttgtggt tgacatgcat
    28861 caacaaagga agccttttag cttcctcgtt gtgcaataga tcaccgtttt ttcggtcgac
    28921 tggcgtacag acagaagaga atggagctgg ttgcgcagaa tgcaattgac caaatcatcg
    28981 gccatgcaga gttaaaggtc gcaagagaaa gcaatgcgcc aacaattgcc cctatgccaa
    29041 aacggaaggt tcctgccagc gaagatgccg ttcccgccat atggggaaac tcatcaagaa
    29101 tgaccgccat cgcattggat gacaccatag acacgcagcc cacaaacgcc gcaacgccaa
    29161 ccaccagcga ccagaatccc agccccagca gcgcactgat gaccatccac gctgccataa
    29221 taaattgtat ccacaacccc gagcggaaca tatttaacgc gccaatgcgg cggacgaagc
    29281 ggctgttaaa gatggtcatc acgaacagaa aaacaatgtt tagcgcaaag taataaccaa
    29341 agttttccgg tgcaacgtgg ttgatttcaa tgtagacaaa cggtccggcg ctcaggaacg
    29401 agaacattcc ggcaaagctg aaaccgctgg ccagcatata actcaggaca cgcttatgac
    29461 ggaacagcgc cgcaaagtta ccaatagtgg tacgaatgtg aaaaggctgg cgaggatcca
    29521 gaagatgtaa tgccagctca gccacaccag cacccagccg ccaactatcg gtgccatcag
    29581 cggtgcaatg gtcgtcacca gcatgacaaa cgacatcatc cgcgagaact cttctttcgg
    29641 gtaaatatca cgcatcaaag cgttgataac cacgctggcc gcagccgcag ccagcccgtg
    29701 gaagaaacgc atcacaatca gctgatcgat ggtttgtgcc aacgcacacg ccaccgcggc
    29761 ggcggcaaac accagcgtac cgccaagaac caccggctta cgcccgaagc tgtctgccat
    29821 cggcccgtag attaactgcc ccaacgcaaa gcccagaata taagtactca gggtcatctg
    29881 cgtactgccc gccggtacgc caaactgcgc tgaaattacc ggtagcgcgg gcagatacat
    29941 atcaatcgac agcggcatca acatggccag caggccaagg ataaaaacaa tagcaaacga
    30001 cgaatgctgt cgggtggtca caacgggctc ctgaaagtca ttgaaaagtt agacgacgct
    30061 ggcaatttct tcttcagtta acggacgata ttcaccgggg gctaaatcag catccagcgt
    30121 aatgccgcca atacgttcac gatgcagctc aaccacatgg ttacccacgg cggcgaacat
    30181 gcgtttcacc tgatgataac gcccttcgct gatggtcaga cgaacctgcg ttggggtaat
    30241 cacttccagc accgccggct tagtgagatc tttttcgtta tgcagctgca cgcctttagc
    30301 aaattgttct gccgtatcgt cagctacggg tgataccagc gtcaccagat aggtcttctc
    30361 gcaatgatgg cgcggagaag taatgcggtg cgaccactga ccatcatctg tcattagtac
    30421 cagaccggtg gtatcgatat ccagccgccc tgcggcgtgc agtttccacg ctaccggttc
    30481 atctagaaaa tagagcaccg ttgggtgatc agggtcgtcc gtggagcaaa cataaccctg
    30541 aggcttattg agcatgaagt aacgtggacc gtgttgctgc gccagcgggt tgccatcgta
    30601 agcgacatca tgttcaggaa gcagtttgaa cgctgcatta cggacgattt cgccatcgac
    30661 ggtgacacga ttgccgcgga tttcacgccc ggcaatagca cggctaacgc cgagttgctg
    30721 tgcgataaat ttatcaagtc gcatgtgtgt gattttgcct gtaaaaaacg gaggtcgggt
    30781 aatgacccga atatctgaac tgttgtctgc ccagtatagc ggtctaataa catccctcaa
    30841 gggaaaaaga tccgtggcat actattagca gaataatcta cctacgcgag accatgattt
    30901 ttacacttcg cccatatcag caagaagccg tggatgccac gctcaaccat tttcgtcgtc
    30961 ataaaacccc tgccgttatc gtgctgccca ccggtgcagg taaaagcctg gtgatagcgg
    31021 aactggcacg gctggctcgt ggtcgcgtac tggtgctggc acacgttaaa gaactggtgg
    31081 cgcaaaacca tgcaaagtat caggcgctgg ggctggaagc cgatattttt gccgccgggc
    31141 tgaagcgtaa agagagccac ggtaaagtgg tatttggcag tgtgcagtcg gtcgcccgta
    31201 atattgatgc ctttcagggt gaattttcgc tgttgattgt cgatgaatgt caccgtattg
    31261 gtgacgatga agagagccag tatcagcaaa tcctcactca cctgacaaaa gtgaatcccc
    31321 acttacgcct gctggggctg actgccacgc cttttcgatt gggcaaaggt tggatctacc
    31381 agttccatta tcacggcatg gtacgcagcg atgagaaagc ccttttccgt gactgcattt
    31441 atgagctgcc cctgcgttat atgattaaac acagctatct gacgccgcca gaacgactgg
    31501 atatgccagt agtgcaatat gatttcagcc gcttgcaggc acagagcaac gggctgttca
    31561 gcgaagccga tctcaaccgt gagctgaaaa aacagcaacg tattaccccg cacatcatca
    31621 gccagattat ggagttcgcc gaaaagcgta aaggagtgat gattttcgcc gcgacggtcg
    31681 aacacgcaaa agagattgtg ggattactgc ctgccgaaga tgcagcactg attactggcg
    31741 acacccccgg cgctgagcgc gatgtgttaa ttgaaaattt taaagcccag cgttttcgct
    31801 atctggtcaa cgtcgcggta ctgaccaccg gatttgacgc cccgcacgtc gatcttatcg
    31861 ccattctgcg ccctaccgaa tcagtgagtc tttaccaaca aattgtcggg cgcggtctgc
    31921 gtctcgctcc gggcaagact gattgtttaa ttcttgatta tgcgggtaat cctcacgatc
    31981 tctacgcgcc ggaagttggt acaccgaaag gcaaaagtga caacgttccg gtacaggttt
    32041 tctgccctgc ctgcggtttt gccaacacct tttgggggaa aacgaccgcc gacgggacat
    32101 tgattgaaca ctttggtcgc cgctgtcagg gatggtttga agatgacgac ggtcatcgcg
    32161 aacagtgtga cttccgtttc cgttttaaaa attgcccgca atgtaacgca gaaaatgata
    32221 ttgccgctcg ccgctgccgc gaatgtgaca cggtactggt tgatccggat gatatgttaa
    32281 aagcggcgct acgactgaaa gatgcgctgg tattacgctg tagcggcatg tctttgcagc
    32341 atgggcacga cgagaaaggc gaatggttga aaatcaccta ttacgatgaa gacggtgcgg
    32401 atgtgagtga gcgtttccgt ctgcaaacac ctgcccagcg taccgccttc gagcagcttt
    32461 ttatccgccc gcatacgcgc acaccgggca tcccgctgcg ctggatcacc gccgccgata
    32521 tcctcgccca gcaagcctta ttgcgacccc cggattttgt cgtcgcccgc atgaaaggcc
    32581 agtactggca ggtgcgtgaa aaagtgttcg attacgaagg tcgttttcgt cgggcgcacg
    32641 aattacgcgg ttaatggtag ttttgattga tgtataaggc gattgagtat agaatctcac
    32701 ccgcttttgc atacgcaaag cagatcactt acctgttgct gggtcgcctg tagcaggatt
    32761 aatttaagag agaaagaaat gtttactatc aacgcagaag tacgtaaaga gcagggtaag
    32821 ggtgcgagcc gccgcctgcg tgccgctaac aagttcccgg caatcatcta cggtggcaaa
    32881 gaagcgccgc tggctatcga gctggatcac gacaaagtca tgaacatgca agctaaagct
    32941 gaattctaca gcgaagttct gaccatcgtt gttgacggta aagaaatcaa agttaaagct
    33001 caggacgtac agcgtcaccc gtacaaaccg aagctgcagc acatcgactt cgttcgcgct
    33061 taattgctga ataagttgta aaaaaccccg ctccggcggg gttttttgta tctgcagatt
    33121 atgcctgatt acggtattgc tattttttgc aggccagata aggcgttcac gccgcatctg
    33181 gcattcagaa cacaaaaccg attaattgcc gccagacgtc cggcgctgca attggtcgcg
    33241 caaattcggc ggcgtgcctt taatggtcag ggtatcggtc gccggctccc agaagatgcg
    33301 ttcacctaac agcatcgcat caaagttaat agtcaagcca ccgccgctac cggcaaactt
    33361 cgtcaactga cgcagtgtgc tccggtctgc cgggaagctc tcttccagtt cgtagccttt
    33421 ttcagccgca aactccgtga agctcacttc gcttacgcct gccagctctt tcgacagcga
    33481 ttccagttcg atctcttcgc ctgcctgcag ctgctcatta cagtagctat aaacctgctg
    33541 gcgcacgttc tgtcgctctg ctttatccag ttgtgcttcg gcagtgaaat catcaaccgc
    33601 ttgcaacagc ccacggtttt gcgctttcgt gttcaatcct tcgctggccc cgaggaaatc
    33661 cataaagaaa tccgccactt tgcgccctac tcgccctttc aggaaagtga gatagcgggt
    33721 ggactctgga ttggtttccc attcggttaa atcgatacgc gcaacaatat ccgcatgatt
    33781 gatatcaaga taatgggttg ggttgatgtc gagattttcg ttaacacgca tactgctcag
    33841 gttgctcaga actgccacca gcagatactc caccgccaga taacggtagt ggcaaaacag
    33901 cacgaatcca ccgtcagcaa aaggatattt cgctaattcg tcacgcaggc gaccagttgc
    33961 cgcgcggcta aatgccagaa agtcctcttc gccctgacgc tgtaagcgca gcgtctgcgc
    34021 cagttcactc tcttcgctaa acaagccgta agctttattt ttggcgctat agacccgatg
    34081 cagttctgcc accatctcaa cgacggtttc tgtcggttcc agcaatgaat cgcgcaacac
    34141 cagctcaagg ttctgctcat cacgcttgat aagctggtgc agagcaatct ggttgatatc
    34201 cagactcatg ataaactctc ctttaagacc gggcggtatt caaccaccgc cggttaagcg
    34261 acgcaataaa agatggcggt cattttacag aaggcagcgt aacgagaatg atttaacgca
    34321 taaaaaagca gaaaaaaaac cgttgctacg gtaatatgtt gccctttcat caactaactg
    34381 atttcgattt atgccacaaa tttcccgcta cagtgatgaa caggttgaac aattgctcgc
    34441 agagctgctc aacgtactgg aaaaacataa ggctccgacc gacctttccc tgatggtgtt
    34501 aggcaacatg gtcactaacc ttatcaacac cagcattgcc ccggcccaac gccaggcaat
    34561 tgccaactct tttgcccgcg ccttacagtc ctctatcaac gaagacaaag cgcactaagg
    34621 gaaacagata acaggttatg gtaactcatc gtcagcgcta ccgtgaaaaa gtctcccaga
    34681 tggtcagttg ggggcactgg tttgcactgt tcaatattct gctttcgctc gtcattggca
    34741 gccgttacct gtttatcgcc gactggccga caacgcttgc tggtcgcatt tattcctacg
    34801 taagcattat cggccatttc agcttcctgg tgttcgccac ctacttgctg atcctcttcc
    34861 cgctgacctt tatcgtcggc tcccagaggc tgatgaggtt tttgtccgtc attctggcaa
    34921 cggcgggaat gacgctatta ctgatcgata gcgaagtctt tactcgtttc catctccatc
    34981 ttaatcccat cgtctggcag ttggttatca acccagacga aaatgagatg gcgcgcgact
    35041 ggcagctgat gttcatcagc gtgccggtta ttttattgct tgaactggtg tttgcgacgt
    35101 ggagctggca aaagctgcgc agcctgacgc gtcgtcgacg cttcgcgcgc ccgctggccg
    35161 cattcttatt tatcgccttt atcgcctcgc atgtggtgta tatctgggcc gatgccaact
    35221 tctatcgccc gatcaccatg cagcgcgcta acctgccgct ttcgtacccg atgacggcgc
    35281 gacgttttct tgagaagcat ggtctgcttg atgcgcagga gtatcaacgc cgtcttattg
    35341 agcaaggtaa tccagacgcc gtttccgttc agtatccgtt aagcgaactg cgctatcgcg
    35401 atatgggcac cgggcagaat gtgctgttga ttactgtcga tggcctgaac tactcacgct
    35461 tcgagaagca gatgcctgcg ctggcaggtt ttgctgagca aaatatttcg ttcacgcgcc
    35521 atatgagctc cggcaacact acagacaacg gcatctttgg cctgttctat ggtatctcgc
    35581 cgagctatat ggacggcatt ctgtcgaccc gtacgcctgc ggcattaatt actgcgctta
    35641 atcagcaagg ctatcagctg gggttattct catcagatgg ctttaccagc ccgctgtatc
    35701 gccaggcatt gttgtcagat ttctcgatgc cgagcgtacg cacccaatcc gacgagcaga
    35761 ccgccacgca gtggatcaac tggctgggcc actacgcaca agaagataac cgctggttct
    35821 cgtgggtttc tttcaatggc actaacattg acgacagcaa tcagcaggca tttgcacgga
    35881 aatatagccg ggcggcaggc aatgtcgatg accagatcaa ccgcgtgctc aatgcactgc
    35941 gtgattctgg caaactggac aatacggttg tgattatcac tgccggtcgg ggtattccgc
    36001 tgagcgaaga cgaagaaacc tttgactggt cccacggtca tctgcaggtg ccattagtga
    36061 ttcactggcc aggcacgccg gcgcagcgta ttaatgcgct gactgatcat accgatctga
    36121 tgacgacgct gatgcaacgc ctgctacatg tcagcacacc tgccagcgaa tattcgcaag
    36181 gtcaggattt gttcaaccct caacgccgtc attactgggt tactgcagcg gataacgata
    36241 cgctggcaat taccaccccg aaaaagacgc tggtgctgaa caataacggt aaataccgca
    36301 cttacaactt acgtggtgaa agagtgaaag atgaaaaacc acagttaagt ttgttattgc
    36361 aagtgctgac agacgagaag cgttttatcg ctaactgatt aattataaat cagttagcga
    36421 aatatcttac ttgcaatcgg tgtggaaaac ggtagtatta gcagccacga gtcggcacgt
    36481 agcgcagcct ggtagcgcac cgtcatgggg tgtcgggggt cggaggttca aatcctctcg
    36541 tgccgaccaa aaatcccaag aaaaaaccaa cctttacggt tggttttttt atatctgcaa
    36601 ttaatttgat aaacagaccg tgacacatca cagcctgttt attttctgtt atcagaacgt
    36661 ccagaccact cccgcctgag tattccacgg cgattctacc cctgcgccat tgccatagct
    36721 gactgacaaa tgaccgctta acgtcggggt aaacgatgac cttatcccag cctgataaac
    36781 gccacgggtt ccagccacat cattattaaa tttgccatcg tcattaactt tcacatggtt
    36841 agaatcggcc tattcctgac gcacggcggc tttcagccag ggttccaacg tcgtaccgtt
    36901 ttgcaggtcc atgtgatagc ttaccgccgt tcccgcttca gcgcgtaata tccgggtatt
    36961 tcccacatcc gcgcgcatgc cgtttgataa cgtgtagtcc tgaccatctg tggtaaagcc
    37021 ggtaaaggcg agataggggc taacactcca caatccgtca acccaacgga acccgctctc
    37081 aacatgagca cccgcgccgt tactattgta atcgccaaac gctgttgccc cattactcat
    37141 cttgccatgg atggtgttgg caaaacggtc aactttcacc accccatcaa cataggcacc
    37201 gttctgatgc tcccaaccgg cataagcccc cagggtgtag ctatcaatat tgcctttgcc
    37261 gccgcgatca aaaccaatat cagaatgaga gtaatcaaag aacaagccgc gaattgtgct
    37321 gctttcttca cgggagaaac ggctatcgat accgagcgtc aggcccgtca atgtttgctc
    37381 aaagccagct cccgcatcag tggtcacgtt gttgcgggtg ttaattgccg aactccacat
    37441 cgccgtatcg taattaacgc cttttacgct accaagacgc tcacgcacgg tgtccagttc
    37501 tgcatcaaat accagcggtt gtgctgctgc catgttcagc acatcagtgg ttgaaggggt
    37561 aatttgcgcg cgattctctg cgaggctcca gctatggttg ccattatcca gcaaggtata
    37621 ttcatacgta ccgatatcaa caacgcctcc ggcattgccc aacgtgaatg cagcatcacc
    37681 gccgcccgtc gttaccagtg taaggctatc tcctgctgcc gggctggccc ccgtgtccgt
    37741 cacgaatatt ttgaaatcac ctgttgcctg accgatgacg ttgagctgat cgctctgatg
    37801 attagccata tcggtacgca tataaaattg ccgtttcccg acagggtatt ggtggtcaac
    37861 gtaatgaaat tttccgcact tgccgtcaca gatgaacgag tcactggctc agccatcata
    37921 atcacatttg cgccggacat cgcaaggttg gctaagttga ggcgagtgtt ttggttcgtc
    37981 gtagtgatat cggcaatgat ggtccatgcg ctattttcga gcgaaacgtc cgcttggctg
    38041 gtatccacgg caccatgcgt tctaaaagaa gcgccctcag aaaccactac cttacccacc
    38101 aacatagcgg gtgcataatt tattttcggc ttcatgacct caagaatgcc atcattccct
    38161 ctgactgaca cgttaaccat tgtgccagcc cagacattag cgcggccatt gttgataacc
    38221 atattttcag agatggcttt agaggaatac gtgatacttc cattctgatc tgatcgaccg
    38281 agatcatata cagcatttgc ctgcactttc gtaccagtat cctttcccag cgattgcata
    38341 gtggcatgct catcaaggat agtgtcaatg gcctgagtgt cctccataac aatgagcccg
    38401 gagccatcat ccagttcata attttttgac acaccatcct ttatactaaa ttggccttta
    38461 ctgtttgtac cactcacttc cagcgcattc gttgagacaa ttaatttccc gcctgctttt
    38521 ttatcaacgc cagttgcagt cccgccatcc ataacctcca gtaagccgcc actatccaca
    38581 gtggtattat aagcgaagtc attttcttca actcgcaaac ttccgccgtt ttccaacagc
    38641 atattgttag caataccatt cttgatttcg aactgaccga gacggtttgt tccgaatacc
    38701 tccatgaccc gcgtggttgt tttaatagca ccgcctgctt tctgatctac cgcaaatgct
    38761 aatcccccgt ctttaacctc cagtaatcct cccttttcga tagcggcaaa gacggcaatc
    38821 ccatcttttt caacaattaa gcgcccgcct ttaatcgtgg gatcattcgc cgcg
//
LOCUS       DAZPDU010000020        38108 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZPDU010000020 DAZPDU010000000
VERSION     DAZPDU010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38108
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.020"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..194
                     /locus_tag="ACHURS_000929"
     CDS             <1..194
                     /locus_tag="ACHURS_000929"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1236724.1"
                     /translation="DSGIPRFESSYPSHIKKARFGEFFAFLRSFNVECDVDTSYPSMS
                     DATLPRLIRPTVGTHPAML"
     gene            complement(197..1372)
                     /gene="ubiF"
                     /locus_tag="ACHURS_000930"
     CDS             complement(197..1372)
                     /gene="ubiF"
                     /locus_tag="ACHURS_000930"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFR1236725.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQFQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            1518..2942
                     /gene="miaB"
                     /locus_tag="ACHURS_000931"
     CDS             1518..2942
                     /gene="miaB"
                     /locus_tag="ACHURS_000931"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFR1236726.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGSTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            3095..4135
                     /locus_tag="ACHURS_000932"
     CDS             3095..4135
                     /locus_tag="ACHURS_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFR1236727.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            4132..4599
                     /gene="ybeY"
                     /locus_tag="ACHURS_000933"
     CDS             4132..4599
                     /gene="ybeY"
                     /locus_tag="ACHURS_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFR1236728.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            4689..5567
                     /gene="corC"
                     /locus_tag="ACHURS_000934"
     CDS             4689..5567
                     /gene="corC"
                     /locus_tag="ACHURS_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFR1236729.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            5592..7130
                     /gene="lnt"
                     /locus_tag="ACHURS_000935"
     CDS             5592..7130
                     /gene="lnt"
                     /locus_tag="ACHURS_000935"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFR1236730.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEVQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(7208..9085)
                     /locus_tag="ACHURS_000936"
     CDS             complement(7208..9085)
                     /locus_tag="ACHURS_000936"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFR1236731.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGTGTYHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDERTLLVPGAGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQMDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            9540..10448
                     /gene="gltI"
                     /locus_tag="ACHURS_000937"
     CDS             9540..10448
                     /gene="gltI"
                     /locus_tag="ACHURS_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFR1236732.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHSDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTTAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            10618..11358
                     /gene="gltJ"
                     /locus_tag="ACHURS_000938"
     CDS             10618..11358
                     /gene="gltJ"
                     /locus_tag="ACHURS_000938"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFR1236733.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            11358..12032
                     /gene="gltK"
                     /locus_tag="ACHURS_000939"
     CDS             11358..12032
                     /gene="gltK"
                     /locus_tag="ACHURS_000939"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFR1236734.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            12032..12757
                     /gene="gltL"
                     /locus_tag="ACHURS_000940"
     CDS             12032..12757
                     /gene="gltL"
                     /locus_tag="ACHURS_000940"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFR1236735.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            12875..13810
                     /gene="rihA"
                     /locus_tag="ACHURS_000941"
     CDS             12875..13810
                     /gene="rihA"
                     /locus_tag="ACHURS_000941"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFR1236736.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            13918..15588
                     /gene="hscC"
                     /locus_tag="ACHURS_000942"
     CDS             13918..15588
                     /gene="hscC"
                     /locus_tag="ACHURS_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HFR1236737.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASDYLAIEIP"
     gene            complement(15648..17099)
                     /gene="djlC"
                     /locus_tag="ACHURS_000943"
     CDS             complement(15648..17099)
                     /gene="djlC"
                     /locus_tag="ACHURS_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HFR1236738.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLCA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGKPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEQDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITQMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EDEKPQSITSIIKISLTVLMIGYALGKIAMLFS"
     gene            complement(17096..17803)
                     /locus_tag="ACHURS_000944"
     CDS             complement(17096..17803)
                     /locus_tag="ACHURS_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR1236739.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            17905..18459
                     /locus_tag="ACHURS_000945"
     CDS             17905..18459
                     /locus_tag="ACHURS_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR1236740.1"
                     /translation="MNKKSMYIFAIFIVAAITCISQPKKTTLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(18469..19896)
                     /locus_tag="ACHURS_000946"
     CDS             complement(18469..19896)
                     /locus_tag="ACHURS_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFR1236741.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKQLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(19893..20600)
                     /locus_tag="ACHURS_000947"
     CDS             complement(19893..20600)
                     /locus_tag="ACHURS_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR1236742.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPALSWQP
                     LADYPTCPETLKDMSDL"
     gene            20764..21741
                     /gene="ybeQ"
                     /locus_tag="ACHURS_000948"
     CDS             20764..21741
                     /gene="ybeQ"
                     /locus_tag="ACHURS_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HFR1236743.1"
                     /translation="MVFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWTLDRGEAPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(21811..22293)
                     /locus_tag="ACHURS_000949"
     CDS             complement(21811..22293)
                     /locus_tag="ACHURS_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFR1236744.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            22528..25110
                     /gene="leuS"
                     /locus_tag="ACHURS_000950"
     CDS             22528..25110
                     /gene="leuS"
                     /locus_tag="ACHURS_000950"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFR1236745.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNMLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVLVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            25125..25706
                     /gene="lptE"
                     /locus_tag="ACHURS_000951"
     CDS             25125..25706
                     /gene="lptE"
                     /locus_tag="ACHURS_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFR1236746.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            25706..26737
                     /gene="holA"
                     /locus_tag="ACHURS_000952"
     CDS             25706..26737
                     /gene="holA"
                     /locus_tag="ACHURS_000952"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFR1236747.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELKGLSLLLCHK
                     PLADVFIDG"
     gene            26739..27380
                     /gene="nadD"
                     /locus_tag="ACHURS_000953"
     CDS             26739..27380
                     /gene="nadD"
                     /locus_tag="ACHURS_000953"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFR1236748.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            27404..28015
                     /locus_tag="ACHURS_000954"
                     /pseudo
     CDS             27404..28015
                     /locus_tag="ACHURS_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
     gene            28275..28592
                     /gene="rsfS"
                     /locus_tag="ACHURS_000955"
     CDS             28275..28592
                     /gene="rsfS"
                     /locus_tag="ACHURS_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFR1236749.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            28596..29063
                     /gene="rlmH"
                     /locus_tag="ACHURS_000956"
     CDS             28596..29063
                     /gene="rlmH"
                     /locus_tag="ACHURS_000956"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFR1236750.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            29094..30995
                     /gene="mrdA"
                     /locus_tag="ACHURS_000957"
     CDS             29094..30995
                     /gene="mrdA"
                     /locus_tag="ACHURS_000957"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFR1236751.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNAPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            30998..32110
                     /gene="mrdB"
                     /locus_tag="ACHURS_000958"
                     /gene_synonym="rodA"
     CDS             30998..32110
                     /gene="mrdB"
                     /locus_tag="ACHURS_000958"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFR1236752.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            32121..33209
                     /gene="rlpA"
                     /locus_tag="ACHURS_000959"
     CDS             32121..33209
                     /gene="rlpA"
                     /locus_tag="ACHURS_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFR1236753.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            33348..34559
                     /gene="dacA"
                     /locus_tag="ACHURS_000960"
     CDS             33348..34559
                     /gene="dacA"
                     /locus_tag="ACHURS_000960"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFR1236754.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            34669..34932
                     /gene="ybeD"
                     /locus_tag="ACHURS_000961"
     CDS             34669..34932
                     /gene="ybeD"
                     /locus_tag="ACHURS_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFR1236755.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            35033..35674
                     /gene="lipB"
                     /locus_tag="ACHURS_000962"
     CDS             35033..35674
                     /gene="lipB"
                     /locus_tag="ACHURS_000962"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFR1236756.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            35934..36887
                     /locus_tag="ACHURS_000963"
     CDS             35934..36887
                     /locus_tag="ACHURS_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFR1236757.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            37096..38061
                     /gene="lipA"
                     /locus_tag="ACHURS_000964"
     CDS             37096..38061
                     /gene="lipA"
                     /locus_tag="ACHURS_000964"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFR1236758.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
BASE COUNT         9545 a         9724 c         9561 g         9278 t
ORIGIN      
        1 ctgattccgg cattccgagg ttcgaatcct cgtaccccag ccacattaaa aaagctcgct
       61 tcggcgagtt ttttgctttt ctgcgttcat tcaatgtcga atgcgatgtt gacacgtctt
      121 atccttcaat gtcggatgcg acgctgccgc gtcttatccg acctacggtt ggcacgcatc
      181 cggcaatgtt gtaaggctac aaccctaacg catatttcag cgcctgacgt ttcaacacgc
      241 cagcacgctc tgccgccatt aacccgagat tacgcacaaa acgcagcggc ggcagattat
      301 tactgaatcc ggcataaaac agatccatac cactttgcat aatgaagtta tccgccatgc
      361 gccgcatctg gtaacgcttg aggacaggat aactggccca cgcttcaccg tagctgcggg
      421 cattcaccag aacatcaatc agggcatcga catcacgata accaagattc accccctgcc
      481 ccgccagcgg atggatggta tgcgcggcat cgcccaccag cgcaagcccc ggctgcacat
      541 actgcaacgc atgtcggcgc gtcagcggaa acgcaccagc ggcaagcggt gtaacgtaac
      601 ccagacgcga cgggaaatgc ttcgcgattt ccgcctggaa ttgcgccata ttcatattct
      661 gcaactggcg aatacgcgcc ggagagtcat accacaccag cgatgcccag ttatcaaaca
      721 acggcagaaa cgcacgcggt ccgtccgggg taaattgctg ccaggtgctg tcgccgggat
      781 cgttctcaca ctgtacgcta atcaacatgc acgactgcgc atactgccag gcatgaacgc
      841 caattcccgc catctgccgc acctgagaat ttgcgccgtc ggcaccaatc accagtttcg
      901 cgcggatcgt ttcaccgccc ttcagttcca gctcctgaag atcattatct cgatgtaatg
      961 caatcagcga gcctggcaca cgtaacgtta ctttcggatg cgcttccagc gcctgccaca
     1021 gcgcctgttg caggacggtg ttttccacca tatagccaag caacggtagc ttaagttcag
     1081 cggcgtcaaa caccacatgc gccgtttccc actcccacgt ttccagtctg cggtaaggat
     1141 ggcaacgcat agcctgtact gcatcccaga cccctaaccc tttaagcaat gataccgatg
     1201 cggcgctgat cgccgagatc cgcacgtccg gttggctatc agcgacaaac ggtgcaggtt
     1261 ctgcgtgctc gatcaccgtt accgaaaatc cgtgctgtgc cagccccagc gccagcgcgc
     1321 cgccgaccat tcctccgccg acaatggcaa tttccgttgg ttgatttgtc atggtcaatc
     1381 atcctgttag caatatgctt taagtttaca ggatttttac ccccaagggc tgataacgct
     1441 catactggtc acaacggcag caaagcatta cactatacgc cctgcattcc tggctactat
     1501 ttcgcaagag caagtcgatg accaaaaaac tccatattaa aacctggggc tgtcagatga
     1561 acgagtacga ttcatcgaag atggccgatc tgctggatgc cacccacggc tatcaactga
     1621 ccgacgtggc ggaagaagcg gatgtgctgc tgctgaacac ctgctcaatc cgcgagaagg
     1681 ctcaggaaaa agtcttccat cagttgggtc gctggaaact gttaaaagag aagaatccag
     1741 acctgattat cggcgtcggt ggctgcgtgg catcgcaaga aggcgagcac attcgccagc
     1801 gcgcccacta tgtcgatatt atttttgggc cgcaaacgct gcaccgtctg ccggagatga
     1861 tcaactccgt gcgcggcgac cgcagcccgg ttgtagatat cagcttcccg gaaatcgaga
     1921 agtttgaccg tctaccggaa ccgcgcgccg aagggccgac cgcgtttgtc tccatcatgg
     1981 aaggctgcaa taaatattgc acctactgcg tggtgcctta cacccgtggt gaagaggtaa
     2041 gccgtccgtc tgacgatatt ctgtttgaga ttgcccagct tgcggctcag ggcgtgcgtg
     2101 aagtcaacct gctcggtcag aacgtgaacg cctggcgtgg tgaaaactac gacggctcca
     2161 ccggatcgtt tgccgatctg ctgcgtctgg ttgctgcgat cgacgggatc gatcgtattc
     2221 gctttaccac cagccatccg atcgaattca ccgacgatat catcgaagtg tatcgcgaca
     2281 cgccggagct ggtgagcttc ctgcatctgc cggtacaaag cggttccgat cgcattctga
     2341 acctgatggg ccgtactcac acggcgctgg agtacaaagc gatcatccgt aaactgcgtg
     2401 cggcgcgtcc ggatattcag atcagttctg acttcattgt tggcttccct ggcgaaacca
     2461 ccgaagactt cgagaaaacg atgaagctga ttgccgacgt caatttcgac atgagctaca
     2521 gctttatctt ctctgcacgt ccgggtacac cagccgccga tatggttgat gatgttccgg
     2581 aagaagagaa gaagcaacgt ctgtatattc tgcaagaacg tatcaatcag caagcgatgg
     2641 catggagccg ccggatgctc ggcaccaccc agcgtattct ggtagaaggg acttcgcgta
     2701 agagcatcat ggagctttcc gggcgtaccg aaaataaccg cgtggtcaac ttcgagggca
     2761 caccggatat gatcggtaaa ttcgtcgatg tagaaattac cgacgtctac ccgaactctc
     2821 tgcgcggtaa agtggtgcgt actgaagatg aaatgggtct gcgcgtggca gaaacaccgg
     2881 aatcggtgat tgcccgtacc cgcaaagaaa acgaccttgg cgtgggttat tatcagccgt
     2941 aattctcagg cccgccgttc cggtgggcct tgttttttct ctctttatcc ccatctttat
     3001 cgcaatgctt gcgccttttt cctgtggcgt gaataatttc cttataggcc ggtgaatgtt
     3061 cagcttccgg cggcatacag ataagaggaa cggtttgaac atagacactc gcgaaatcac
     3121 cctggagcca gcagacaacg cgcgtctgtt gagcctgtgc ggcccgtttg atgacaacat
     3181 caagcagctc gaacgccgtc tcggcatcga gatcaatcgc cgcgacaacc actttaaact
     3241 gaccggccgt ccgatttgcg tcaccgctgc ggcagacatt ctgcgtagcc tgtatgtcga
     3301 tacagccccg atgcgcggtc agattcagga tatcgaaccg gaacagatcc accttgcgat
     3361 taaagaagcg cgagtactgg agcaaagtgc ggagagcgtg ccggagtacg gcaaagcggt
     3421 caatatcaaa accaaacgcg gcgtaattaa gccgcgcacg ccaaaccagg cgcagtacat
     3481 cgccaatatt ctcgaccatg acattacctt cggcgttggc ccggcgggta cgggtaaaac
     3541 ctacctggca gtggctgcgg cagttgatgc cctggagcgt caggagattc gccgtattct
     3601 gctgactcgt ccggcggtcg aagccggtga gaaactgggc ttcctgcctg gcgatttaag
     3661 ccagaaagta gacccgtatc tgcgcccact gtacgacgcg ctgtttgaaa tgctgggctt
     3721 tgagaaagtt gagaaactga ttgagcgcaa cgttattgaa gtcgcgccgc tggcctatat
     3781 gcgtggtcgt acgctgaacg acgcgtttat cattctcgat gagagccaga acaccaccat
     3841 cgaacagatg aagatgttcc tgacccgtat cggttttaac tcaaaagcgg ttatcaccgg
     3901 cgacgtcaca cagatcgacc tgccgcgtaa tactaaatca ggcttacgtc acgccatcga
     3961 agtgctggcc gatgtcgaag agatcagctt taacttcttc cacagcgaag acgtggttcg
     4021 tcaccctgtg gtggcgcgta tcgttaacgc ctatgaagcc tgggaagaag ccgaacaaaa
     4081 acgaaaagcg gcgctggcgg cagaacgcaa gcgcgaagaa caggaacaaa aatgagtcag
     4141 gtgatcctcg atttacaact ggcatgtgaa gataattccg ggttaccgga agagagccag
     4201 tttcaaacat ggctgaatgc ggtgatcccg cagtttcagg aagaatcgga agtgactatt
     4261 cgcgtggtcg ataccgccga aagccacagt ctgaatctga cctatcgcgg taaggataag
     4321 ccgaccaacg tgctctcctt cccgtttgaa gtgccgcctg gcatggaaat gtcgctactg
     4381 ggcgatctgg ttatctgccg tcaggtggtt gagaaggaag ctcaggagca aggcaaacca
     4441 ctggaggcgc actgggcgca tatggtggtg cacggcagtc tgcatttgtt aggttacgat
     4501 cacatcgaag atgacgaagc agaagaaatg gaagccctcg aaacagagat tatgcttgct
     4561 ctgggctatg aggatccgta cattgccgag aaagaataat ttgccaggca tttgactggc
     4621 agacgtcccc gccgttggtg attaacgacg gcgttttgat taactaacat gagaacccat
     4681 acgacgccat gagcgacgac aattcacaca gtagtgacac gataagcaac aagaagggat
     4741 ttttctccct gttactcagc caacttttcc acggtgaacc gaaaaaccgt gacgaactgc
     4801 tggcgctgat ccgtgattcc gggcagaacg accttatcga cgaagatacg cgcgatatgc
     4861 tcgaaggggt gatggacatc gcagaccaac gcgttcgcga catcatgatc ccccgctccc
     4921 agatgattac cctgaaacgc aaccagacgc tggacgaatg ccttgatgtc atcatcgagt
     4981 ccgcccactc acgtttcccg gtgattagcg aagacaaaga tcacattgaa gggattctga
     5041 tggcgaaaga cttgctgccg tttatgcgca gcgatgctga agccttcagc atggacaaag
     5101 tgttacgtca ggcggttgtc gttcctgaaa gtaagcgcgt agaccggatg ctgaaagagt
     5161 ttcgctctca gcgttaccac atggcgatcg tcattgacga attcggtggg gtttccggtc
     5221 tggtgaccat tgaagacatc ctggaactga ttgttggtga gattgaagac gagtatgacg
     5281 aagaagatga tatcgacttc cgtcagctga gtcgtcatac ctggaccgtg cgtgcactgg
     5341 cttccattga agacttcaac gaagcgttcg gcacccactt tagcgatgaa gaggttgaca
     5401 ctatcggtgg tctggtgatg caggcatttg ggcatcttcc ggcgcgtggc gaaactatcg
     5461 acatcgacgg ttaccagttc aaagtggcga tggccgacag tcggcgtatt attcaggttc
     5521 atgtcaaaat cccggatgac tcaccccagc cgaagctgga tgaataaaac cgaaactgga
     5581 tagataacta catggctttt gcctcattaa ttgaacgcca gcgcattcgc ctgctgctgg
     5641 cgttattatt cggtgcctgc ggaacgctgg ccttctctcc ttacgacgtc tggcctgcgg
     5701 cgattatttc gttgatgggg cttcaggcgt tgacctttaa ccgccgtcca ctccagtctg
     5761 ccgctattgg cttttgctgg ggatttggcc tctttggcag cggtattaac tgggtctatg
     5821 tcagcatcgc gacctttggc ggaatgcctg gcccggttaa catcttcctg gtggtgctgc
     5881 tggcggcgta tttgtcgctg tataccggac tgtttgctgg cgtgctgtcg cgtctgtggc
     5941 cgaaaaccac ctggctgcgc gtggcgattg ccgcccctgc tctctggcaa gtgaccgagt
     6001 ttctgcgcgg ttgggtactg acaggcttcc cgtggttaca gttcggctat agccagattg
     6061 atggcccgtt aaaagggctg gcaccgataa tgggcgtgga agccattaac ttcctgctga
     6121 tgatggttag tggcctgctg gcactggcgt tggtcaaacg caactggcgt ccgctggtgg
     6181 tggccgtcgt gctgtttgcc ctacccttcc cgctgcgtta catccaatgg tttaccccgc
     6241 aaccggagaa aaccattcag gtttcgatgg ttcagggcga tattccgcaa tcactgaaat
     6301 gggatggaga tcagcttctt aatacgctga agatttacta caacgcaacg gctccgctga
     6361 tgggcaaatc atcgttgatt atctggccgg agtcggcgat aaccgatctg gaaattaatc
     6421 agcaaccgtt cctcaaagca ctggacggtg agttgcgcga taaaggtagc tcgctggtaa
     6481 ccgggattgt cgacgcgcgt ctcaataagc agaaccgcta cgatacctac aacaccatca
     6541 tcacactggg taaaggtgca ccatacagct acgaatcagc cgatcgctat aacaaaaacc
     6601 atctagtgcc gtttggcgag tttgtcccgc tggagtcgat tctgcgtccg ttagcaccgt
     6661 tctttgatct gccgatgtcg tcgttcagcc gtgggccata tatccagccg ccgctgtcgg
     6721 taaatggtat tgagcttact gcggctattt gctacgagat cattctcggc gagcaagtgc
     6781 gcgataactt ccgcccggat accgactatc tgctgactat ctccaacgat gcgtggtttg
     6841 gtaagtctat tggtccatgg cagcacttcc agatggcgcg aatgcgtgcg ctggagctgg
     6901 cgcgcccact gttgcgcagc accaacaacg gcattacggc ggtgattggc ccgcaggggg
     6961 aggttcaggc gatgatcccg cagttcaccc gcgaggtgtt gaccactaat gtgacaccga
     7021 ccaccggact cacaccgtat gcacgtaccg gcaactggcc actgtgggtg ctgacggcgc
     7081 tgtttggttt tgccgctgtg ttgatgagtc tgcgagcacg taaacgttga tgtgcctgat
     7141 gtatcctgca tgagcaaaac gtactttctc attgagcttt ggggcatggt tatcatgccc
     7201 ctttttctta cggttcaggg atacaagcca cattgccata ttggggatga cgaataattg
     7261 cctgcggatc aagcgagcgc gtcctgtcca tttggatcaa cccgttcttc gccatgacaa
     7321 atatttttct accatccgct gaaaaacgaa ggtgattaac gtagctggca ggataaacgc
     7381 caataatctg cccggcatta atattgagca ggatgacata accttgcagg ccatatgaca
     7441 ccaacgctaa atattgaccg tccgggctaa taccctcaac ggaataaacg ctcctgctac
     7501 accattgcgt cataatgctg gcttgttgat cccctacgct tatacgtgaa atcccctgac
     7561 tatcggcaaa aataacctcg ccgttttcag gtcgaaaacg catcgccgat atcgttccac
     7621 gcgcatcacc tgcgggaaag tcttctttct gatgcgtgat ggtattgatc cgcacgatgt
     7681 tacctctgcc tgctcccgga accagtaatg ttctttcatc cggggaaagt gccataaccc
     7741 aggcgcctgg tttaccgctc gtccccgtcc cgtcgtattt ctccagcggg atatccgcca
     7801 catgctgata agtcaccaga tcaatcgctg acacactatt actggtgcga ttcatcaccc
     7861 aggcttttgt gccatcacgc gagaggataa ggctgtcagg atgtgcaccg gtggtaatcg
     7921 actgaataat ctcccctgtc gccacgttaa tgaccgataa cgcattcgct tcaaatgaag
     7981 tcgcataaat caacttttca tccgggctta atgccagcga tctgacgccc tgatctttac
     8041 agccaccaca atgtttgact gacttttctt tcgctatggg cgctgtaaag gtatgcagga
     8101 ttttcttttg tccgaggtcg aaaacaacaa tgctgttcgt gttttcaatt gcggcataca
     8161 acatattacc atcccgactg atcgccatat catggatatc ggcaatctca gcgagcaaat
     8221 caccactggc ctgttcacga cttaccggag cggataatgt ttccctgagg gcgttgcgtt
     8281 cctccgctaa ctgctgcttt ttttgccgtc gctcttgttc aatctcctgt ggataaaacg
     8341 cttcacgcag gctttgcctg acctgtaaaa ctgactcatc catctgctgc tgcgcaagcc
     8401 agatagcccc ggtcagaaga agactgcccg caacaataat cccgccttcc gtaacgagtc
     8461 ggttttgccc agataaacgc gcgctcagcc aacccaacgc gccacccgca atcaggccgc
     8521 caatgtggca agcgttatct attcctgttt gccgggcacc gtacaacagt gtcagcgcca
     8581 ccatacccaa cagaggaaat actcgccgct ggtttttatg gtatgtgccc gtacctgaag
     8641 caaactgtgt tgctatcgct gcaccagcta ttcccagaat cgccccggat gccccaacgc
     8701 tgatgactat ctgctcactt tcgcgtaacg cataacaggc gctgatgagc ccaccaaaga
     8761 cgccggagaa taaccaggta atcaggagtc gccatttccc cagaatctgt tccgccacgc
     8821 ctcccacgac caggaatgca aacatattca tcagcagatg agaaacacta ctgtgcagaa
     8881 agatactgct caataagcgc cagggttcac ccgacaaagt aagaggcgca acgtttgcgc
     8941 cgagatggat aagtgcggat tgatcgacct cttcataact catccattgt gcgagataac
     9001 aaaatatccc aatacagagt gcaaaaagga taagggttat tgcgggtaat tgaacattta
     9061 acggcttaac cgaagatgca gacataaggt gtttcttcct tgaaaagaca ggtaaaaagc
     9121 atttttcccg tgtgcagtta tcatcccggc aaacgtccca tgccgaaaag ataattctgt
     9181 ccgaaatatg actttttcag cctaaaatta caatcgtaat taattcgccc ctaagtattt
     9241 tttggcatgt aagttgcttt atttactgtg taagcgatga aatcagccgc gacgcaacca
     9301 ggttgcacca ccacaaatca gcggtagcac caaaaaggtg cggtttaaga attttgcttc
     9361 ggaatggtgc ggcgcatctc gcaaaaacaa acaataaaac agcaatatct ttacatttag
     9421 ccaaactaaa tgttacctaa caggcacaac actgcacaat aaagttgcag acgataacaa
     9481 cacaaacact cacaacgggt atccatgcgt tcttaacgca gaagataaag gagttggata
     9541 tgcaattacg taaacctgcc acagcaatcc tcgccctggc gctttccgca ggactggcac
     9601 aggcagatga cgccgccccg gcagcaggca gtacgctgga caaaatcgcc aaaaacggtg
     9661 tgattgtcgt cggtcaccgt gaatcttcag tacctttctc ttattacgac aatcagcaaa
     9721 aagtggtggg ttactcgcag gattactcca acgccattgt tgaagcagtg aaaaagaaac
     9781 tcaacaaacc ggacttgcag gtaaaactga ttccgattac ctcacaaaac cgtattccac
     9841 tgctgcaaaa cggcactttc gattttgaat gtggttctac caccaacaac gtcgaacgcc
     9901 aaaaacaggc ggctttctct gatactattt tcgtggtcgg tacgcgcctg ctgaccaaaa
     9961 agggcggcga tatcaaagat tttgccgacc tgaaagacaa agccgtagtc gtcacttccg
    10021 gcactacctc tgaagttttg ctcaacaaac tgaatgaaga gcaaaaaatg aatatgcgca
    10081 tcatcagcgc caaagatcac agtgactctt tccgcaccct ggaaagcggt cgtgccgttg
    10141 cctttatgat ggatgatgcc ctgctggccg gtgaacgtgc gaaagcgaag aaaccagaca
    10201 actgggaaat cgtcggcaag ccgcagtctc aggaagccta cggttgcatg ttgcgtaaag
    10261 acgatccgca gttcaaaaag ctgatggatg acaccaccgc gcaggtgcag acctccggtg
    10321 aagcggaaaa atggtttgat aaatggttca aaaatcccat tccgccgaaa aacctgaaca
    10381 tgaatttcga actgtcagac gaaatgaaag cactgttcaa agaaccgaat gacaaggcac
    10441 tgaactaatt acaagaacca ggggcggaaa ttccagccct ctcgattgtt acgtagcacg
    10501 gacagactat acgcctgatg gtcgttcccc atcgggcctg aaaaccgcaa tacgctgggt
    10561 aacaatcttc gagggtagca gttaacgctg ctaccctctt ttttctggag tagatttatg
    10621 tctatagact ggaactgggg tattttttta caacaagccc cgttcggcaa caccacctat
    10681 cttggttgga tctggagtgg ttttcaggtg acgatcgctt tgtcgatctg cgcctggatt
    10741 atcgctttcc tcgtcggttc atttttcggc attttacgta ccgttccaaa ccgttttctc
    10801 tccggtctgg gtacgttgta tgtcgaactg ttccgcaacg tgccgttgat tgtgcaattc
    10861 tttacctggt atctggtgat cccggagctg ttgccggaga aaatcggcat gtggtttaag
    10921 gccgagctgg atcccaatat tcagtttttc ctttcatcca tgctctgcct ggggctgttt
    10981 accgccgccc gtgtttgcga acaggtacgc gccgccattc agtcgttgcc gcgtgggcaa
    11041 aaaaatgccg cgctggcgat gggcctgacg ctaccgcagg cttatcgata cgtactgctg
    11101 cctaatgctt atcgcgttat cgtcccgccg atgacttccg agatgatgaa cctggtgaaa
    11161 aactccgcca tcgcctcgac tattggtctg gtggatatgg cggcgcaggc gggtaaattg
    11221 cttgattact cagcccacgc ctgggagtcg tttaccgcta ttacgctggc gtatgtgttg
    11281 attaacgcgg ttatcatgct ggtaatgacg ctggttgaac gtaaagttcg cctgcctggc
    11341 aacatggggg gcaaataatg tacgagtttg actggagttc cattgtccct tccctgccat
    11401 atctgctcga cgggctggtg atcacgctga aaatcaccgt cacggcagtc gtgattggta
    11461 ttttgtgggg caccatgctg gcggtgatgc gcttgtccag ctttgcgccc gtagcgtggt
    11521 ttgccaaagc ctacgttaac gtattccgct cgattccttt agttatggtt ttgctgtggt
    11581 tttacctgat cgtgccaggt tttctgcaaa acgtgctggg attatcacca aaaaatgata
    11641 tccgtttaat ttcggcgatg gtggcgtttt cgatgtttga agcggcctac tattcagaga
    11701 ttatccgtgc tggtattcaa agtatttctc gtggtcagtc gagcgccgcg ctggcgttgg
    11761 gaatgactca ctggcagtcg atgaaactga ttattctgcc gcaggcgttc cgcgcgatgg
    11821 tgccgcttct gctcactcag ggcatcgtac tgttccagga tacctcactg gtgtatgtgt
    11881 tatctctggc cgatttcttc cgtaccgcct caaccattgg tgagcgtgat ggtacgcagg
    11941 ttgagatgat cctgtttgcc ggatttgttt atttcgttat tagtcttagc gcgtcgttgt
    12001 tggtcagcta cttgaaaaga aggacagcat aatgattacc ctgaaaaatg tttcaaaatg
    12061 gtatggtcac tttcaggtgc tgaccgactg ctcaaccgaa gtgaaaaaag gcgaagtggt
    12121 ggtggtttgc ggcccgtctg gttccggcaa atcaacgctg attaaaaccg tcaacggcct
    12181 cgaaccagtg cagcaaggtg aaatcaccgt cgatggtatc gtggttaatg acaagaaaac
    12241 cgatctggca aagctgcgtt cccgcgtcgg gatggtattc cagcatttcg agctgttccc
    12301 tcatctgtcg attatcgaaa acctgaccct ggcgcaggtg aaagtgctta aacgcgataa
    12361 agcgcccgct cgtgaaaaag cattgaaatt gctggagcgc gtgggacttt ccgcacatgc
    12421 caataaattt ccggcgcagc tttccggcgg tcagcagcag cgtgtggcaa tcgctcgcgc
    12481 attgtgtatg gatcctattg cgatgctgtt cgacgaaccg acatcggcgc tggatccgga
    12541 gatgatcaac gaagtgctgg acgtgatggt ggaactggcg aacgaaggaa tgaccatgat
    12601 ggtggtgacc cacgaaatgg gctttgcccg caaagtggcg aatcgggtga tctttatgga
    12661 cgagggtaaa attgtcgaag actcgccgaa agacgctttc ttcgatgatc cgaaatcgga
    12721 ccgcgcgaaa gacttcctcg cgaaaatcct gcattaatca ctctggcgcg catacccaag
    12781 tgcgcgccat ctcacgcttt atcgtttcgc ccctcgtatc tgttacatcc tggcgttacc
    12841 cttgtcgcaa agaagcacac aacaaggagc aacaatggca ctgccaattc tgttagattg
    12901 cgacccaggt catgacgacg ctatcgcaat agttctcgcc ctcgcctcac cagagcttga
    12961 tgtcaaagca attacgtctt ccgccggaaa ccagacacca gaaaaaacct tacgcaatgt
    13021 tctgcgtatg ctgaccttgc ttaatcgcac cgatattccg gtagcaggcg gcgcggtaaa
    13081 accgttaatg cgtgagttga ttatcgcgga caatgtgcac ggcgaaagcg gtctggatgg
    13141 cccggcatta ccggaaccga cgttcgcacc gcaaaactgt acggcggtag agctgatggc
    13201 gaaaacgctg cgcgaaagtg cggaacctgt cacgattgtg tctaccggac cgcaaactaa
    13261 cgttgccttg ctgctcaata gccatccgga actgcatagc aaaattgccc gtatcgtgat
    13321 tatgggcggc gcaatggggc ttggtaactg gacgcctgcg gctgaattta atatttacgt
    13381 tgacccggaa gcggcagaaa ttgtcttcca gtcagggatc ccagtggtga tggccggtct
    13441 ggatgttact cacaaagcac aaatccacgt tgaagacacc gagcgtttcc gcgcgattgg
    13501 caaccctgtt tcaaccattg ttgccgaact gctggatttc ttcctcgaat atcataaaga
    13561 cgaaaaatgg ggctttgtcg gtgcaccgct gcatgaccca tgcaccatcg cctggctgtt
    13621 gaaacccgag ttgtttacca ctgttgagcg ctgggttggc gtggaaacac aggggaaata
    13681 tagccagggt atgacggttg ttgattatta ttatctgact ggcaataaac cgaatgccac
    13741 tgtaatggtc gatgttgatc gtcagggctt tgttgattta ctggctgatc gtctgaaatt
    13801 ttacgcttaa cacacgtcat tgattaagat caccacgcgt tacctgggtg gtcttaatct
    13861 gtttatctgt ttatctgttt atctgtttat ctctttttct ttcaagatat aaatgcaatg
    13921 gataatgcag aactcgccat tggtatcgat ctcggcacta ccaatagttt aattgccgtc
    13981 tggaaagacg gtgccgcgca attaattcca aataagttcg gtgaatattt aacaccatcc
    14041 ataattagca tggatgaaaa taatcatatt ttagtcggaa aaccggctgt atcacggcgt
    14101 acttcgcatc cggataaaac ggcagcgtta tttaaacgtg caatgggcag taataccaac
    14161 tggcggttag gcagcgacac atttaacgcg ccagaactgt cctctttggt attacgctca
    14221 ttaaaagaag atgccgaaga atttctgcaa cgtccgatta aagatgtggt gatctccgtt
    14281 ccggcttatt tcagcgatga acaacgcaag catacccgtt tagcagcgga gttagccggg
    14341 ttaaatgcgg tacgcttaat taatgaaccc acagcagctg cgatggcata tggcctgcat
    14401 acgcaacaaa atacccgctc gctggttttt gatctcggtg gcggcacgtt tgacgtcacg
    14461 gtgctcgaat acgccacgcc ggtgattgaa gttcacgcct ccgctggcga taacttcctc
    14521 ggtggcgaag attttaccca tatgttggtc gatgacgttt taaagcgcgc ggatgtcgcc
    14581 aggaccacgc ttaacgagag tgaactggca gccttgtacg cctgtgtgga agcggcaaaa
    14641 tgtagcaatc aatcgccatt gcacattcgc tggcagtacc aggatgaaac gcgggagtgc
    14701 gaattttacg agaacgaact ggaagatttg tggttgccgc tgctcaatcg cttgcgagtg
    14761 ccgattgaac aggcgttgcg cgatgcacgt ctgaagccga gtcaaatcga cagtctggtg
    14821 ctggttggcg gcgcgtcaca aatgccgctg gtgcagcgaa tcgccgtgcg tctgtttggc
    14881 aaattaccgt atcaaagtta cgacccgagc accattgtcg cgctgggcgc agcaatccag
    14941 gccgcctgcc gcttacgcag tgaagatatt gaagaggtga tcctcactga tatttgccct
    15001 tactcgttgg gcgttgaagt taaccgccag ggcgtttccg gcattttctc gccaattatt
    15061 gaacgaaaca ccacagtacc cgtgtcgcgt gtagaaactt actcgaccat gcacccggaa
    15121 caggattcta ttacggttaa cgtctatcag ggagaaaacc acaaagttaa aaataacatt
    15181 ctggttgaat ccttcgatgt gccgttgaag aaaaccgggg cttatcagtc gattgatatt
    15241 cgctttagtt atgatatcaa cggcttgctt gaagttgacg tgcttctgga agatggcagc
    15301 gttaagtcca gagtcattaa tcatagcccg gtaacattga gcgcgcagca gattgaagag
    15361 agtcggacgc ggttatccgc attgaaaatt tatccgcgcg atatgctaat caaccgcacc
    15421 tttaaggcca aactggaaga gttgtgggcg cgggcgctgg gtgacgagcg agaagagatt
    15481 ggccgggtga tcaccgattt tgatgcggcg ttgcagtcaa acgatatggc ccgcgtcgat
    15541 gaagttcgtc gacgggcgag cgattatttg gctattgaga tcccttaaac acggctgccg
    15601 gatgcggcat aaacgctgca tccggcatcg gcgcaaaaaa cacatcctca gctaaacaac
    15661 atcgcgattt ttccgagcgc ataacctatc atcagcaccg ttaagctgat tttgataata
    15721 ctggtaatgc tctgtggttt ttcatcctca tcattttttt cttcatggtt ttcaggtgtt
    15781 ggctcatcga catcttcatc ctccggctcg agtttcactt tcgtcaacag catctgggtg
    15841 ataatggcct ctaatggatg agcatctcca ccttccagcc ttgtattaag acgggccagc
    15901 atctcttgtt tcgggaacgc ttccagcaca acctgttccg ctacccgata caaatccagt
    15961 aacggtgaat cttgctctgt ctcgctaccg actctcgatt tccaccacgc ccatgctttg
    16021 cgcttgaaga ataacgactc aataaacgat tgacctaacg gcgataattt tccgctatgt
    16081 aaaatctccg cccataacag ccgcgtccgg gcgctctcac caggaacata tttcaacggt
    16141 ttaccttgcg catctaccat gcttcgcgct tcgataacca tcactaataa cgcgaaataa
    16201 tccgggcaat gacgcttgct ccactgcaac aacaaattat cagcttgtgt agatgggtaa
    16261 ctttcccagt acgcgcagag atcggcaagc aggctttccc cctcgccgca gtacaaacgt
    16321 tgtgcacact ggtaataccg ggcatcttcg ctttctggtt cttccgcttg ccactgtttg
    16381 gctacaggaa tgagttccgc catacctgtt tgcaccgagc tgtaccagcg caacagtttg
    16441 cgatgtaact ttgcatcatc aggaatgaac cacggtcctt ccatcgccaa aaacgcagtg
    16501 acatagtcag gatggtaacg gcagcaacgt tccagcgcga agtaatattc aacagtctgg
    16561 ttctgtaacg cgacgggata ttccagaagg ctaaggaaat cgaaacaatc accagcctga
    16621 atggcctcaa gaaaagcctc cctttcttcc tcatccattc cttcgctgtc attaaacgaa
    16681 tgccagttca aacgctctgc caacaaagaa gcacaattta atgaaagata tcgcgcttct
    16741 atagcgattg cacacagcgg ccagcgtaat tgatcgacat cctccatgtt ccaggtattt
    16801 aattgctgaa taaatttctg ccaggcggag ggctgaaaac gatcactttc tgaatccagt
    16861 aatgtcctga atgcacgtag tatttcatgt tcagcggtgg catctttttc ttcatcatct
    16921 gcttcctcga caggatttac cgccagtcgc agcgcctctt cataggcctg gcgtaatgct
    16981 ttaaacccct gcggatcggt ttcgggatga cacaacggta agcgagcaag ataagcctgg
    17041 cggataatgt ctatttgcgt cgtgctttca atttcgagga tttgccaaca tgttttcata
    17101 ggtcgctcac atccttaagc gttaccgggc attcaggctg gacagttaat ggttgccagg
    17161 gaagcgtggc atagaaacgg tcgcttcccg ctgccagctc ttcgaacatc cgactcccgg
    17221 cgctatcgta tttgtacagg gttagcgcct cgcgtagcga agcgttgtct tcgcactgag
    17281 aagattgcca gtagagacgc cctagtgaat aggcagaaaa atagtgcatc cagctgtgat
    17341 agtaatggtg agctcgtgca tagacgcgcg attgcagcca taaactctct tcttccgtca
    17401 gccagttgtt gagcacaccg acccgtgaca gaaaccccat tcgaacataa tcccaggctt
    17461 tgattccccc ttctccacag cacatagcgg tttgggcaac gaattgggta taaatcaacc
    17521 ctctctccga gccaccagca attaatgctt tccactcttc aggggaagag cgttgccatg
    17581 tcagatataa gccagctaaa tcattggcat gaccatcatc agtcatctga taaatagtct
    17641 gaattaaatc atcacgatta tttattcccc aggatgatga gagatcgaat tcttttatcc
    17701 cagtatacat ttttggcgag tcatgcgcgg gtttgcaact ctgattaagt atttccatgg
    17761 gcgcagaaag cgcaaaaaga agatattgtt cttccttatt cattttttaa taatccctta
    17821 agtgcattaa ctgactactt ttttattatt gatgagtata aacttgacgc tttcaaaata
    17881 aaaagaaaat cgaagcattc acacatgaat aaaaaatcaa tgtatatatt cgcaattttt
    17941 atagttgcag caattacctg tattagccaa cccaagaaaa cgacgttgcg tgataaagcc
    18001 atggtgaatt atgcctttga ttatttaaga tcaccaggca gtctaccgtt aaccacggca
    18061 gccacggagc tttccgcgat tcatggtcac tcaacatcgc aatatcgcct tggcgaattt
    18121 tatcttcatg gtagcgacgg taaaccactg gattatacac aggcgagata ctggtatgag
    18181 caatcagcag aacaggaaaa tccgcgcgca caaagcaaac tggggtggat ctacctgaaa
    18241 ggcctgggag tcaaacccga tacccgtaaa gcaattctct ggtataagga agcagctgaa
    18301 caagggtatg ctcatgctca atatactttg ggtttgatct acagaaatgg cactggtatt
    18361 aatgttaacc attatgaatc tcaaaaatgg ttaaaactgg ccgccaaaca acattacaaa
    18421 aatgcggaaa gattacttgc cggacttccc gcacattaac gtcgactgtc acccgaagag
    18481 atggagtatt tttcctatca aaccggcaaa gataaaaatg tagaaaataa ttttgatgac
    18541 gcccagacag cccagcccgc catcgtcaac gggcattggc tgcggttgcg gctccggctc
    18601 gacggtttcc tgaccggtaa aattcgccgc ctgaattaat aatcgacgaa taattaatgc
    18661 ttccagtgga caacgatctt ctgcatcaag gcggctcaca atcggcatta atgattcttc
    18721 tgtcggttgg ctggcctgca ctaattgttt ggcaactaaa tataatggcc ataagggatg
    18781 ttctgtatca cgcgtgagtg agatccgcgg cttacgtcct ttgtgcaaaa ccatctcgac
    18841 cagtgaacgc cccagcgcag agagtttttc atcgctgagt atttcgtata aatgaaaacg
    18901 cgtacgggcg ctgtcgccgg ggacataaag tagctgttta ccctgatcgt taaccagttc
    18961 acgagcttca atcatcatca ccactaacgg gtaataatcg acccgatgct gacggcacca
    19021 ttgcaatatt aaagcatccg cctgggtgga gggatactcg cgccagtagt cacaaagttc
    19081 tgccagcaag ctttcgcctt ctccacaata gacgcgttgc gcgtattcat aataacgcgc
    19141 atcttcatta tcaggttcag cttcccgcca cgaccgggcg taatccagaa gttcggggat
    19201 atccaggcga cctgcgctat accagcggag taaatttctg tgcagttttg catcatcggg
    19261 gatgatcact gcgcgatgtt gcgctaaata caatgtcagc cattctggat gcgatgacca
    19321 aatccgagcg aacatttggt aaaaatcgat ggtgtcattt tgcacggcaa cgggcagatg
    19381 cagaatggtc tggaagttaa aaacattccc cttcgcaatg gcatataaaa aggattccag
    19441 ttcttcttcg tctatttccc cgacgttttc ctcctgcaac caccgcaatc tttctgctaa
    19501 taacaccacg cactcgaagg ataaatgggc agtgttcatg gcgattgtgc acagcgacca
    19561 gcgtaattca tcaatctctt ccatcgagca ataatttaat tgctgaatga atcgctgcca
    19621 ggcggagggc agaaaacgtt cgctatcaga ggcaagtaac gcgcgaaagg cgagcagaat
    19681 ttcatgttct gctacctcat attcttctgg ttgccaaaca gatttagcag gcgactgcgc
    19741 aatccggagc gcctcctcat acgcctgacg aagttgttta aaaccctgcg gatcggtttc
    19801 tggatgaaag gacggtaaca gcgccagata agcgcggcgg ataatatcga cgtcagtcgt
    19861 ttcctctatt tcgaggatct tccagcaatt tttcataggt cgctcatatc cttaagcgtt
    19921 tccgggcatg tggggtagtc agccagtggt tgccaggaca aggcagggta atacgcatca
    19981 tcactggcaa ataattgata aaacatatca ttgccggaat cgtcgtattc tttacgagct
    20041 aagaattcgc gtaataaagg cagatgatca tcttcgggag actgccaata ttgccgacca
    20101 aaggtataac cggcaaagta ttgccgccag ttactataat aacgtagagc ccgtagatga
    20161 atacgcgact ggatccatag actttcttct tcactcaacc agttattaag tacgcccatt
    20221 cgacttaaaa aacccatccg gacataatcc catgctttaa ttcctccctc accacagcat
    20281 tcggctgtgc tggcgacaaa ttgcgcataa gcctgacctt gctcatttag ttcagcaaga
    20341 tagtcacgcc attcacatgg cgaataacga aaccagcggt gataaaaccc tgccaaccga
    20401 gcggcatgac cgttatcgat cattcggtga attgtgcgca gcaggtcatc acggtcatca
    20461 attccccatg aggaagaaag gtcaaaaaag catgtaccta aatacatttt gggggatgaa
    20521 tgcgaaggta aacagcattc attgcgtatt tccatcggag tggaaagtgc aaacaatatt
    20581 ttttgcgacc ccatgtccat ataatttatc cttaaacatt catttaatga atgtaaacta
    20641 catttcgaga ctcagttatt tatacttttc tgacgcaaca taatacgcat aaaataaggc
    20701 ttacgcaaaa atggaacaca taaaaatata atatgtttta tctacaacca taaggaaatg
    20761 ataatggttt ttacgtcaag ttgctgcgat aatttatcaa tagatgagat tatcgaacgt
    20821 gctgaaaaag gcgattgcga ggctcaatat attgttgggt tttattataa tcgcgatagc
    20881 gcaattgact ctccggacga cgaaaaggcc ttttactggc tgaagctggc cgctgagcaa
    20941 ggtcattgtg aagcacagta ttccttaggg caaaagtata ccgaggataa aagccgtcat
    21001 aaagataatg agcaagccat cttctggctg aaaaaagctg ccctacaagg ccatactttc
    21061 gcttccaacg cccttggctg gacactggat cgtggagaag cccccaatta taaagaagcg
    21121 gttgtctggt atcagatagc cgcggagagc ggaatgtctt atgcgcaaaa taatcttggg
    21181 tggatgtaca gaaatggcaa cggagtcgca aaagactatg cgctggcatt tttctggtac
    21241 aaacaagctg cattacaagg ccatagtgac gcgcaaaaca atctggccga tctttatgaa
    21301 gacggaaaag gcgttgctca aaacaagaca ctcgccgcat tctggtattt gaaaagcgca
    21361 cagcagggta atcggcacgc ccagtttcaa attgcatggg attataacgc tggcgaaggg
    21421 gtggaccagg actataagca agcgatgtac tggtatctga aggctgccgc tcaggaaagc
    21481 gtcggcgctt acgtcaacat cggttatatg tataaacacg gacaaggcgt tgagaaagat
    21541 tatcaggctg cctttgaatg gtttacgaaa gccgctgaat gcaatgacgc cactgcctgg
    21601 tataacctgg ccattatgta tcactacgga gaaggaagac ctgtcgatct ccgacaggct
    21661 ctcgacctgt atcgtaaagt tcagtcatcc ggaaccagag atgtcagtca ggaaattcgt
    21721 gagactgaag atttactgta gagataaaaa caagggccgg ggaatttcat tcggcccata
    21781 ttctctttat tgctgtgcga aatcagacta ttacggctca aacgggcgtc tctggaactg
    21841 gtcatgacca catttcgggc atagcgtcag cacttccggt gtgtagatcg ggagatggaa
    21901 gtgacatttc tcgcagacca gatttcccag cccgaccact tctccgctgt gataaacccc
    21961 atgatgattg aggtcctgga aaacttcgcg ccattcaagc tgcgttttat cggtgatatc
    22021 tgccagctcc tgccataagc tttctttaat cacccgcata aagacgctgt cagattcttc
    22081 tttcaggctc tcttcatagc tcatggcgaa ctcttccagg tcacgtctta cagctcgcgt
    22141 cagctcatcg acctcggttc gcgttaactc ccctgttttt attacgcgct cgcgcgcctg
    22201 ttccaccagt gcgtcgatat cacgttcgcc attgcgcagg cgttcgctca gtgacgcgac
    22261 cagttcacgg taatattgag caaccttgtt catcgtttca tctcccgggt cgttaactga
    22321 ctataataat agacgttctt tgttccttgc tttgctaata cgcccacaaa ctctgtaaat
    22381 taatgtatga ataatccggg catcatttca acgtcgagtg cgcgaaaggc tgttttgacg
    22441 cggacgtttg ggctatgcta tgcggatctg aaaaaccacc tcaacgctac atttgtagcc
    22501 gtattgaaaa caggaccact ggctgccatg caagagcaat accgcccgga agagatagaa
    22561 tccaaagtac agcttcattg ggatgagaag cgcacatttg aagtaaccga agacgagagc
    22621 aaagagaagt attactgcct gtctatgctt ccctatcctt ctggtcgact acacatgggc
    22681 cacgtacgta actacaccat cggtgacgtg atcgcccgct accagcgtat gctgggcaaa
    22741 aacgtcctgc agccgatcgg ctgggacgcg tttggtctgc ctgcggaagg cgcggcggtg
    22801 aaaaacaaca ccgctccggc accgtggacg tacgacaaca tcgcgtatat gaaaaaccag
    22861 ctcaaaatgc tgggctttgg ttatgactgg agccgcgagc tggcaacctg tacgccggaa
    22921 tactaccgtt gggaacagaa attcttcacc gagctgtata aaaaaggcct ggtatataag
    22981 aagacttctg cggtcaactg gtgcccgaac gaccagaccg tactggcgaa cgaacaagtt
    23041 atcgacggct gctgctggcg ctgcgatacc aaagttgaac gtaaagagat cccgcagtgg
    23101 tttatcaaaa tcactgctta cgctgacgag ctgctcaacg atctggataa actggatcac
    23161 tggccagaca ccgttaaaac catgcagcgt aactggatcg gtcgttccga aggcgtggag
    23221 atcaccttca acgttaacga ctatgacaac atgctgaccg tttacactac ccgcccggac
    23281 acctttatgg gttgtaccta cctggcggta gctgcgggtc atccgctggc gcagaaagcg
    23341 gcggaaaata atcctgaact ggcggccttt attgacgaat gccgtaacac caaagttgcc
    23401 gaagctgaaa tggcgacgat ggagaaaaaa ggcgtcgata ctggctttaa agctgttcac
    23461 ccattaacgg gcgaagaaat tcccgtttgg gcagcaaact tcgtattgat ggagtacggc
    23521 acgggcgcag ttatggcggt tccgggtcac gaccagcgcg actacgagtt tgcctctaaa
    23581 tacggcctga acatcaaacc agttatcctg gctgctgacg gctctgagcc agatctttct
    23641 cagcaagccc tgactgaaaa aggcgtgctg ttcaactctg gcgaattcaa cggtcttgac
    23701 catgaagcgg ccttcaacgc catcgccgat aaactgactg cgatgggcgt tggcgagcgt
    23761 aaagtgaact accgcctgcg cgactggggt gtttctcgtc agcgttactg gggcgcgccg
    23821 attccgatgg tgacgctgga agacggtacc gtaatgccga ccccggacga ccagctgccg
    23881 gtgatcctgc cggaagatgt ggtgatggac ggcattacca gcccgattaa agcagatccg
    23941 gagtgggcaa aaactaccgt taacggtatg ccagcgctgc gtgaaaccga cactttcgac
    24001 acctttatgg agtcctcctg gtactatgcg cgctacactt gcccagagta caaagaaggt
    24061 atgctggatt ccgaagcggc taactactgg ctgccggtgg atatctacat tggtggtatc
    24121 gaacacgcca ttatgcacct gctctacttc cgcttcttcc acaaactgat gcgtgatgca
    24181 ggcatggtga actctgacga accagcgaaa cagttgctgt gtcagggtat ggtgctggca
    24241 gatgccttct actatgttgg cgaaaacggc gaacgtaact gggtttcccc ggttgatgct
    24301 atcgttgaac gtgacgagaa aggccgtatc gtgaaagcga aagatgcggc aggccatgaa
    24361 ctggtttata ccggcatgag caaaatgtcc aagtcgaaga acaacggtat cgacccgcag
    24421 gtgatggttg aacgttacgg cgcggacacc gttcgtctgt ttatgatgtt tgcttctccg
    24481 gctgatatga ctctcgaatg gcaggaatcc ggtgtggaag gggctaaccg cttcctgaaa
    24541 cgtgtctgga aactggttta cgagcacaca gcaaaaggtg atgttgcggc actgaacgtt
    24601 gatgcgctga ctgaagatca gaaagcgctg cgtcgcgatg tgcataaaac gatcgctaaa
    24661 gtgaccgatg atatcggccg tcgtcagacc ttcaacaccg caattgcggc gattatggag
    24721 ctgatgaaca aactggcgaa agcaccaacc gatggcgagc aggatcgcgc tctgatgcag
    24781 gaagcgctgc tggccgttgt ccgtatgctt aacccgttca ccccgcacat ctgcttcacg
    24841 ctgtggcagg aactgaaagg cgaaggcgat atcgacaacg cgccgtggcc ggttgctgac
    24901 gaaaaagcga tggtggaaga ctccacgctg gtcgtggtgc aggttaacgg taaagtccgt
    24961 gccaaaatca ccgttctggt ggacgcaacg gaagaacagg ttcgcgaacg tgctggccag
    25021 gaacatctgg tagcaaaata tcttgatggc gttaccgtgc gtaaagtgat ttacgtacca
    25081 ggtaaactcc tcaatctggt cgttggctaa gcgcgggagg aagcgtgcga tatctggcaa
    25141 cattgttgtt atctctggcg gtgttaatca ccgccgggtg tggctggcat ctgcgtgata
    25201 ccacgcaggt tccttccact atgaaggtca tgatcctgga ctcaggcgat ccgaacgggc
    25261 cattaagccg tgcagtgcgt aaccagttac gtctgaatgg tgtcgagttg cttgataaag
    25321 aaaccacgcg taaggacgtt ccatccctgc gtttgggtaa agtgagcatc gcgaaagata
    25381 ccgcatcggt attccgtaac ggtcaaacag cagagtatca gatgatcatg acggtgaatg
    25441 cgaccgtgtt gatccccggc cgtgatatct acccgattag cgccaaagtc ttccgttcgt
    25501 tctttgataa cccgcaaatg gcgttagcga aagataacga acaagacatg atcgtaaaag
    25561 agatgtacga ccgtgctgcc gaacagctga ttcgtaagct gccaagcatc cgtgctgcgg
    25621 atattcgttc cgacgaagaa cagacgtcga ccacaacgga tactccggca acgcctgcac
    25681 gcgtctccac cacgctgggt aactgatgat tcggttgtac ccggaacaac tccgcgcgca
    25741 gctcaatgaa gggctgcgcg cggcgtatct tttacttggt aacgatcctc tgttattgca
    25801 ggaaagccag gacgctgttc gtcaggtagc tgcggcacaa ggattcgaag aacaccacac
    25861 tttttccatt gatcccaaca ctgactggaa tgcgatcttt tcgttatgcc aggctatgag
    25921 tctgtttgcc agtcgacaaa cgctattgct gttgttacca gaaaacggac cgaatgcggc
    25981 gatcaatgaa caacttctca cactcaccgg acttctgcat gacgacctgc tgttgatcgt
    26041 ccgcggtaat aaattaagca aagcgcaaga aaatgccgcc tggtttactg ctcttgcgaa
    26101 tcgcagcgtg caggtgacct gtcagacacc ggagcaggct cagcttcccc gctgggttgc
    26161 tgcgcgcgca aaacagctca acttagaact ggatgacgcg gcaaatcagg tgctctgcta
    26221 ctgttatgaa ggtaacctgc tggcgctggc tcaggcactg gagcgtttat cgctgctctg
    26281 gccagacggc aaattgacat taccgcgcgt tgaacaggcg gtgaatgatg ccgcgcattt
    26341 cacccctttt cattgggttg atgctttgtt gatgggaaaa agtaagcgcg cattgcatat
    26401 tcttcagcaa ctgcgtctgg aaggcagcga accggttatt ttgttgcgca cattacaacg
    26461 tgaactgttg ttactggtta acctgaaacg ccagtctgcc catacgccac tgcgtgcgtt
    26521 gtttgataag catcgggtat ggcagaaccg ccggggcatg atgggcgagg cgttaaatcg
    26581 cttaagtcag acgcagttac gtcaggccgt gcaactcctg acacgaacgg aactcaccct
    26641 caaacaagat tacggtcagt cagtgtgggc agagctgaaa gggttatctc ttctgttgtg
    26701 ccataaaccc ctggcggacg tatttatcga cggttgatat gaaatcttta caggctctgt
    26761 ttggcggcac ctttgatccg gtgcactatg gtcatctaaa acccgtggaa acgctggcga
    26821 atttgattgg tctgacgcgg gtcacaatca tccctaataa tgttcctccg catcgtcccc
    26881 agccggaagc gaacagcgtg cagcgtaaac acatgcttga actggcgatt gccgacaagc
    26941 cattatttac tcttgatgaa cgcgagctaa agcgcaatgc cccctcttac actgcgcaaa
    27001 cactgaaaga gtggcggcag gaacaaggac cggacgtgcc gctggcgttt attattggtc
    27061 aggattcact gctgaccttt ccgacctggt acgaatacga aacgatactc gacaatgcac
    27121 atttgatcgt ctgtcggcgt ccaggttacc cacttgaaat ggcgcaaccg caataccagc
    27181 aatggctgga agatcatttg acacataacc cggaagatct tcaccttcag cctgccggta
    27241 aaatttatct ggctgaaacg ccgtggttta acatctcggc gaccatcatc cgcgaacgtt
    27301 tgcaaaacgg tgaatcgtgt gaggatttat tgccggaacc ggtactgact tacattaacc
    27361 aacaaggctt gtatcgctga tacctgtcgt tacggagggc atgatgcgac tgtggttaat
    27421 tcgtcatggt gaaacgcaag cgaatatcga tggtctttac agcggtcatg cgcccacccc
    27481 cctgaccgcg cgcggtattg agcaagcgca aaatctgcat acgctgctac atggtgtttc
    27541 ctttgatctg gttttatgca gtgaactgga acgggcacag cataccgcac gactggttct
    27601 cagtgaccgc cagctccccg tgcaaatcat ccctgaactc aacgaaatgt tttttggcga
    27661 ctgggagatg cgacatcatc gcgacctcat gcaagaagat gccgaaaact atagcgcgtg
    27721 gtgcaatgac tggcagcatg caatccccac gaacggtgaa ggatttcagg cattttcgca
    27781 acgtgtggaa cgctttatcg caaggcttag tgaatttcag cactatcaga atattttagt
    27841 cgtcagccat cagggtgtac tgagtctgtt aatcgcccgt ttaattggca tgcctgccga
    27901 ggctatgtgg cattttcgcg ttgactaggg gtgttggagc gccattgata tcaaccaaaa
    27961 attcgcgacg ctacgcgtcc tcaatagccg tgccatcggg gtcgagaatg catgactttt
    28021 ctgttttttt acgggtaagc cgcaacgacc attgacagac ccgggcaggc tgatattctc
    28081 cgcagccaga cttttttcgc cagacacgac tttgtagaaa ttgttttaca aaaatggcga
    28141 tgcaatctgc ggcgcggggt gggatgatag cccactttcg aaagccgatt cggcgacaat
    28201 tgtcccgaaa tcgcctctgg ttcaggtata ctgacagacc atttttatct atttgattca
    28261 cccaggggga aaacttgcag ggtaaagcac tccaggattt tgttatcgac aaaattgatg
    28321 acctcaaagg tcaggacatc atcgccttag acgttcaagg caaatccagc atcaccgact
    28381 gcatgatcat ctgtacgggt acgtccagcc gtcatgttat gtccattgct gaccacgttg
    28441 tgcaggagtc tcgcgcagcg ggcctgttac cgctcggcgt agaaggtgaa aacagcgccg
    28501 actggattgt cgtggatttg ggcgatgtga ttgtccatgt catgcaggaa gagagccgtc
    28561 gcctgtatga actggaaaaa ctctggagtt aatgcgtgaa gctgcaactt gtcgccgtgg
    28621 gaacgaaaat gccggactgg gtacaaaccg gttttaccga gtacctgcgt cgttttccga
    28681 aagatatgcc cttcgagctg attgaaattc cggccggaaa acgcggcaag aatgcggaca
    28741 tcaagcgcat actcgacaaa gagggtgagc agatgttggc ggccgcaggc aaaaaccgca
    28801 ttgtcaccct cgatattcca ggcaagccct gggatacgcc gcagttagcc gctgagctgg
    28861 aacgctggaa gctggatggt cgcgacgtca gtctactgat tggcgggcct gaagggttgt
    28921 cgcctgcctg taaagcggcg gcggagcaga gctggtcgct gtcggcgctt accctccccc
    28981 atccgctggt tcgcgtgctg gtcgcagaga gtctgtaccg ggcgtggagc atcaccacca
    29041 accatcctta tcaccgtgag tgataaggga gctttgagta gaaaacgcag cggatgaaac
    29101 tacagaactc ttttcgcgac tatacggcag agtccgcgct gtttgtgcgc cgggcgctgg
    29161 tcgccttttt ggggattttg ctgctgaccg gcgtgcttat cgccaacctg tataatctgc
    29221 aaattgttcg ctttaccgac taccagactc gctctaatga aaaccgcatt aagctggtgc
    29281 ctatcgcgcc cagccgcggc attatctacg accgtaatgg tatccctctg gccctcaacc
    29341 gcactatcta ccagatagaa atgatgccag agaaagtcga taacgtgcag caaacgctgg
    29401 acgctttgcg cagcgtggtt gatctgaccg atgacgatat tgctgcattc cgaaaagagc
    29461 gcgcacgttc acaccgtttc acctctattc cggtgaaaac taacctgacc gaagtacaag
    29521 tagctcgctt tgccgtcaat cagtaccgtt ttccgggtgt cgaagttaaa ggctataaac
    29581 gtcgttacta tccttacggt tcggcgttga cccacgtcat cggctatgtg tcgaaaatca
    29641 acgataaaga cgtcgaacgc ctgaataatg acggcaaact ggccaactat gcggcaacgc
    29701 atgatatcgg taagctgggc attgagcgtt actatgaaga cgtgctgcac ggtcagaccg
    29761 gttatgaaga ggttgaagtt aacaaccgtg ggcgtgttat ccgccagtta aaagaagtac
    29821 caccgcaagc cggacacgat atttacctga cgctggatct caaactccag caatatattg
    29881 aaacgctgct ggcgggtagc cgcgcagctg tggttgtcac cgatccgcgt acaggtgggg
    29941 tgctggcgct ggtttccacg cctagttatg acccaaacct gtttgttgac ggtatctcca
    30001 gcaaagatta ttccgccttg ttgaacgccc cgaatacacc gctggtgaac cgcgccacac
    30061 agggggttta tcctcccgcg tctacagtta aaccctatgt ggcggtttcg gcattgagcg
    30121 ccggggtgat cacgcgcaat acgacgctgt ttgacccagg ctggtggcaa ctgccaggtt
    30181 cggaaaaacg ttatcgtgac tggaaaaaat ggggccacgg gcgtctgaat gtcacaagat
    30241 cgctggaaga atctgcggat accttcttct atcaggtggc ctacgatatg gggatcgatc
    30301 gcctctccga atggatgggt aaattcggtt atggtcatta caccggtatc gacctggcgg
    30361 aagaacgttc cggcaacatg cctacccgcg aatggaaaca gaaacgcttt aaaaaaccgt
    30421 ggtatcaggg tgacaccatt ccggttggta tcggtcaggg ttactggaca gcgaccccaa
    30481 tccagatgag taaggcactg atgatcctga ttaatgacgg tatcgtgaag gttcctcatt
    30541 tgctgatgag caccgccgaa gacggcaaac aggtgccatg ggtacagccg catgaaccgc
    30601 ccgtcggcga tattcattcc ggttactggg agctggcgaa agacggtatg tacggtgttg
    30661 ctaaccgccc taacggtacg gcgcataaat actttgccag cgcaccgtac aaaattgcgg
    30721 cgaaatccgg taccgctcag gtcttcggtc tgaaagcgaa cgaaacctat aatgcgcaca
    30781 aaattgccga gcgtttacgt gaccacaaac tgatgaccgc ctttgcgcca tacaacaatc
    30841 cgcaagtggc tgtcgccatg attctggaga acggtggtgc gggtccggcg gttggtacac
    30901 tgatgcgcca gatcctcgac cacattatgc tgggtgataa caacaccgat ctgcctgcgg
    30961 aaaatccagc ggttgccgca gcggaggacc attaatcatg acggataatc cgaataaaaa
    31021 aacattctgg gataaagtcc atctcgatcc cacaatgctg ctgatcttac tggcattgct
    31081 ggtttacagc gccctggtta tctggagcgc cagcggtcag gatattggca tgatggagcg
    31141 taaaatcggc caaatcgcga tgggtctagt catcatggtg gtgatggcgc aaattcctcc
    31201 acgcgtttat gaaggctggg ccccctatct ctatatcatc tgtattattt tgctggtggc
    31261 ggtagatgct ttcggtgcca tctctaaagg tgctcaacgc tggctggacc tcggtattgt
    31321 tcgttttcag ccgtcggaaa ttgccaaaat agccgtacca ctgatggttg cgcgctttat
    31381 caaccgcgac gtttgcccgc catcgttgaa gaacactggc atcgcgctgg tgctgatatt
    31441 tatgcccacg ctgctggtgg ctgcacagcc tgacctggga acatcaatcc tcgttgcgct
    31501 ttccggtctg tttgtactgt tcctctctgg ccttagctgg cgtctgattg gcgtcgcagt
    31561 agtgctggtg gcggcgttca ttccgattct gtggttcttc ctgatgcatg attaccagcg
    31621 ccagcgcgta atgatgctcc tagacccgga atcagaccca ctcggcgcgg gctatcacat
    31681 tattcagtct aaaattgcta ttggctccgg cggattacgc ggcaaaggct ggctgcacgg
    31741 cactcagtca cagcttgaat ttctccccga acgccatacc gactttatct tcgcggtact
    31801 ggcggaagag ctgggattag tgggcattct gattctgctc gctctctaca ttctgctgat
    31861 catgcgcggg ctgtggatag ccgccagagc gcaaaccacc tttggtcgcg tcatggctgg
    31921 tggcttaatg ctgatattat tcgtttatgt cttcgtaaat attggtatgg taagcggtat
    31981 tctgccggtt gtaggggttc cgctcccact ggtcagttat ggaggatcgg cgctaattgt
    32041 gctgatggct gggttcggga ttgtaatgtc aatccacacc cacaggaaaa tgttgtcgaa
    32101 aagcgtgtaa gaggtgcgca atgcgtaagc agtggctcgg gatctgcatc gcggcaggaa
    32161 tgctcgcggc atgtacaagc gatgatggtc agcaacagac ggtaagtgta ccgcagcctg
    32221 cggtatgtaa cggccccata gttgaaatta gcggggcgga cccgcgtttc gaaccactga
    32281 acgcgacggc aaatcaggat taccagcgcg acggtaaaag ctacaaaatc gtgcaggatc
    32341 cgtctcgatt tagccaggcg ggactggcgg caatctatga tgccgaaccc ggcagtaacc
    32401 tgacggcctc tggcgaagct ttcgatccga cacagctgac ggcggcccat ccaacgcttc
    32461 cgatccccag ctacgccaga atcactaacc tggctaacgg gcgaatgatc gtggtgcgca
    32521 ttaatgatcg cggcccttac ggcaacgacc gcgttatttc actttctcgc gcggcagctg
    32581 accgtcttaa cacgtcaaac aacaccaaag ttcgtatcga tccgattatt gtcgcccagg
    32641 atggttcgct ttctggtcct ggtatggctt gtaccacagt cgccaaacag acttacgccc
    32701 tgccagcacc tcccgattta agcggtggcg cgggaacaag ttcagtgtct ggcccgcagg
    32761 gtgacattct tccggtcagt aattcgacgc taaaaagcga agatccgacc ggcgcgccgg
    32821 taaccagtag cggtttcctc ggcgctccaa cgaccttagc gcctggtgta ctggaaggca
    32881 gcgaaccgac gcctgcgcca cagcccgttg ttacagctcc gtcgacaacg cctgcaacct
    32941 cgcctgcaat ggtgacaccg caagccgcct cgcaaagcgc cagcggcaac tttatggtgc
    33001 aagtcggggc cgtaagcgat caggctcgtg cgcaacagta ccaacagcaa ctgggacaga
    33061 agttcggcgt ccccggtcgc gtaactcaaa atggcgcggt ctggcggatc cagcttggcc
    33121 cattcgccag caaagccgaa gccagtacct tgcagcaacg tttgcaaacc gaagcccaat
    33181 tacagtcatt tattactacc gcgcagtagc gtaaagcagg catctgatgt gtcaatttct
    33241 gtaaatgacg ttaacaaagt cacgcggaaa gtcagatgcc tgccggtagt gcatttgcta
    33301 tagtagggca cttttttaat tccatcacgg atgtcgtagt tcagaccatg aataccattt
    33361 tttccgctcg tatcatgaag cgcctggcgc tcaccacggc tctttgcaca gcctttatct
    33421 ctgctgcaca tgccgatgac ctgaatatca aaactatgat cccgggtgta ccgcagatcg
    33481 atgcggagtc ctacatcctg attgactata actccggcaa agtgctcgcc gaacagaacg
    33541 cagatgtccg ccgcgatcct gccagcctga ccaaaatgat gaccagttac gttatcggcc
    33601 aggcaatgaa agccggtaaa tttaaagaaa ctgatttagt cactatcggc aacgacgcat
    33661 gggccaccgg taacccggtg tttaaaggtt cttcgctgat gttcctcaaa ccgggcatgc
    33721 aggttccggt ttctcagctg atccgcggta ttaacctgca atcgggtaac gatgcttgtg
    33781 tcgccatggc cgattttgcc gctggtagcc aggacgcttt tgttggcttg atgaacagct
    33841 acgttaacgc actgggcctg aaaaataccc acttccagac ggtacatggt ctggatgctg
    33901 atggtcagta cagctccgcg cgagatatgg cgctgatcgg ccaggcattg atccgtgacg
    33961 taccgaatga atactcgatc tataaagaaa aagaatttac gtttaacggt attcgccagc
    34021 tgaaccgtaa cggcctgtta tgggataaca gcctgaatgt cgacggcatc aaaaccggac
    34081 acactgacaa agcaggttac aaccttgttg cttctgcgac tgaaggccag atgcgcttga
    34141 tttctgcggt gatgggcgga cgtactttta aaggccgtga agccgaaagt aaaaaactgc
    34201 taacctgggg cttccgtttc ttcgaaaccg ttaacccact gaaagtaggt aaagagttcg
    34261 cctctgaacc ggtttggttt ggtgattctg atcgcgcttc gttaggggtt gataaagacg
    34321 tgtacctgac cattccacgt ggtcgcatga aagatctgaa agccagctat gtgctgaaca
    34381 gcagtgaatt gcatgcgccg ctgcaaaaga atcaggtcgt cggaactatc aacttccagc
    34441 ttgatggcaa aacgatcgag caacgcccgc tggttgtgtt gcaagaaatc ccggaaggta
    34501 acttcttcgg caaaatcatt gattacatta aattaatgtt ccatcactgg tttggttaaa
    34561 aattgaacac ttgaaagtgt aatttccgtc cccatatact aagcatcagt aaaaaactcc
    34621 cgccttctgg cgggagttgc tatttaatta cgttacgccg gagctgacat gaaaaccaaa
    34681 cttaacgaac tgcttgaatt ccctactcct tttacttaca aagttatggg gcaggcgtta
    34741 cctgagctgg ttgatcaggt ggttgaagtg gtacagcgcc atgcgccagg tgactacacc
    34801 ccaacggtaa aaccaagcag caaaggcaac taccactcgg tatctatcac tatcaacgcc
    34861 actcatatcg agcaggttga aacactgtat gaagaactag gcaaaatcga tattgtccgc
    34921 atggttctgt aactcgcttc tccgttaccc ggcccctggt cgggtaactc ccctctcctc
    34981 gctgtgatat acttttcccc cacttttact cattctccac ggagatgcag ttttgtatca
    35041 ggataaaatt cttgtccgcc agctcggtct tcagccttac gagccaatct cccaggctat
    35101 gcatgaattc accgataccc gcgatgatag tacccttgat gaaatctggc tggtcgagca
    35161 ctatccggta ttcacccaag gtcaggcagg aaaagcggag cacattttaa tgccgggtga
    35221 tattccggtg atccagagcg atcgcggtgg gcaggtgact tatcacgggc cggggcaaca
    35281 ggtgatgtat gtgttgctta acctgaaacg ccgtaaactc ggtgtgcgtg aactggtgac
    35341 cttgcttgag caaacagtgg tgaataccct ggctgaactg ggtatagaag cgcatcctcg
    35401 ggctgacgcg cctggtgtct atgtcgggga aaagaaaatt tgctcactgg gtttacgtat
    35461 tcgacgcggt tgttcattcc acggtctggc attaaacgtc aatatggatc tttcaccatt
    35521 tttacgtatt aatccttgtg ggtatgccgg aatggaaatg gctaaaatat cacaatggaa
    35581 acccgaagcg acgactaata atattgcacc acgtttactg gaaaatattt tagcgctact
    35641 aaacaatcct gacttcgaat atattaccgc ttaattgcat acctcaatgg cccaatttac
    35701 actgggtcat tactcataat acctgctaat gctttacatc tccttcgcgt ttgtatactt
    35761 tctttatcac ttgcagaaat gcggtgctac gccttgctcc gtaactcacc ccattgatta
    35821 acctattgaa atagttgatt atggtgagca aaagcgttcc gttccaatta gacataagat
    35881 tattgtaatt tttttaaata ttatttttcc atgaataaaa ttggagtaag tgcgtggata
    35941 gtaataatca aattgaaccc tgtttaagca gaaagtcgtc agaaggtaaa ccacaaatat
    36001 ttacaacctt acgaaatatt gatcttaacc ttctgactat ttttgaagct gtatatgtac
    36061 ataaagggat cgttaatgca gcgaaagtgc ttaatctgac cccctcggca atcagtcagt
    36121 ctattcagaa actgcgcgtt atattccctg acccattgtt tattcgcaaa ggccagggtg
    36181 tcactcctac cgcatttgcg atgcatctac atgagtatat cagtcagggc cttgagtcca
    36241 ttcttggcgc gctggatatc gaaggaagct atgataagca acgaacgata actattgcga
    36301 ccactccctc ggtcggagcc ctggtccttc ctgtcatcta tcgggcgatt aaaactcact
    36361 atccgcagct tttactgcgc aacccgccca tcagcgacgc ggaaaaccaa ctcagtcagt
    36421 ttcaaaccga tctcatcatc gataacatgt tttgcaccaa tcgtacggtg caacatcatg
    36481 ttctgttcac cgacaatatg gtgttaattt gccgcgaggg aaatccacta ctctctttag
    36541 aagatgacag agagactatc gacaacgctg cgcatgtact cctgttaccg gaagggcaaa
    36601 atttcagcgg tctgcggcag agagttcaag agatgtttcc ggaccggcaa atcaatttca
    36661 ccagctacaa cattttgaca atcgctgcac tggttgccaa cagtgacatg ttagcgatta
    36721 ttcccagccg tttttataac ctgtttagtc gttgctggcc gctggaaaaa ctgccttttc
    36781 cgtccttaaa tgaggagcaa atagatttct ctatccacta caacaaattc agcctgcgtg
    36841 atcccatcct gcacggggta atagatgtta tccgtaatgc attttgaaaa agtattgtta
    36901 cagccagatc acagcgcaga acgataaaat ggcacaatcc aacaacaatt ttacattttc
    36961 gcgaccgctt tggctgcttt caggtccgtt tcaatgatat actgccagtc gttaattcaa
    37021 aaatagttga taattacaac aatctattga attgaaacgc tttccttcgt aattcgcaac
    37081 tggaacacgc acgctatgag taaacccatt gtgatggaac gcggtgttaa ataccgcgat
    37141 gccgataaga tggcccttat cccggttaaa aacgtggcaa cagagcgcga agccctgctg
    37201 cgcaagccgg aatggatgaa aatcaagctt ccagcggact ctacacgtat ccagggcatc
    37261 aaagccgcaa tgcgcaaaaa tggcctgcat tctgtctgcg aggaagcctc ctgccctaac
    37321 ctggcggaat gcttcaacca cggcacagca acgtttatga tcctcggcgc tatttgtacc
    37381 cgccgttgtc cgttctgtga tgttgcccac ggtcgcccgg tagctcctga tgccaatgaa
    37441 ccagtgaaac tggcgcagac cattgccgat atggcgctgc gttatgtggt tatcacctcc
    37501 gttgaccgtg atgacctgcg cgatggcggt gcccagcact ttgcggattg cattactgcc
    37561 attcgggaaa aaagcccgca aatcaaaatt gaaactctgg tgccggattt ccgtggtcgt
    37621 atggatcgtg ctctggatat tctgactgca acgccaccag atgtgttcaa ccataacctg
    37681 gaaaacgtac cgcgtattta ccgtcaggta cggcctggtg cagattacaa ctggtcgctg
    37741 aagctgctgg aacgctttaa agaagcgcat ccggaaatcc cgaccaagtc tggtctgatg
    37801 gtgggactgg gtgaaaccaa tgaagaaatt attgaggtaa tgcgcgacct gcgccgtcat
    37861 ggtgtgacga tgttaacgct ggggcaatat ttgcagccaa gccgccatca cctgccggtt
    37921 caacgttacg ttagcccgga tgagttcgac gaaatgaaag ccgaagcgct ggcgatgggc
    37981 tttacccatg ctgcatgcgg tccgtttgtc cgctcttctt accacgccga tttgcaggcg
    38041 aaagggatgg aagttaagta atcctgtaat aattacttac atctgtctga taaaaaaccc
    38101 gctttttg
//
LOCUS       DAZPDU010000021        37274 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZPDU010000021 DAZPDU010000000
VERSION     DAZPDU010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37274
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.021"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(268..885)
                     /gene="tdk"
                     /locus_tag="ACHURS_000965"
     CDS             complement(268..885)
                     /gene="tdk"
                     /locus_tag="ACHURS_000965"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFR1236759.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1490..1903
                     /gene="hns"
                     /locus_tag="ACHURS_000966"
     CDS             1490..1903
                     /gene="hns"
                     /locus_tag="ACHURS_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFR1236760.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2047..2955)
                     /gene="galU"
                     /locus_tag="ACHURS_000967"
     CDS             complement(2047..2955)
                     /gene="galU"
                     /locus_tag="ACHURS_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFR1236761.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3157..4170)
                     /gene="rssB"
                     /locus_tag="ACHURS_000968"
     CDS             complement(3157..4170)
                     /gene="rssB"
                     /locus_tag="ACHURS_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFR1236762.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4262..5167)
                     /gene="rssA"
                     /locus_tag="ACHURS_000969"
     CDS             complement(4262..5167)
                     /gene="rssA"
                     /locus_tag="ACHURS_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFR1236763.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5280..5738
                     /locus_tag="ACHURS_000970"
     CDS             5280..5738
                     /locus_tag="ACHURS_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFR1236764.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5788..6630
                     /gene="purU"
                     /locus_tag="ACHURS_000971"
     CDS             5788..6630
                     /gene="purU"
                     /locus_tag="ACHURS_000971"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFR1236765.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMAEAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6790..6874
                     /locus_tag="ACHURS_000972"
     tRNA            6790..6874
                     /locus_tag="ACHURS_000972"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6824..6826,aa:Tyr,seq:gta)
     gene            6922..7053
                     /locus_tag="ACHURS_000973"
     ncRNA           6922..7053
                     /ncRNA_class="other"
                     /locus_tag="ACHURS_000973"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7237..7914)
                     /gene="narI"
                     /locus_tag="ACHURS_000974"
     CDS             complement(7237..7914)
                     /gene="narI"
                     /locus_tag="ACHURS_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFR1236766.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7914..8624)
                     /gene="narJ"
                     /locus_tag="ACHURS_000975"
     CDS             complement(7914..8624)
                     /gene="narJ"
                     /locus_tag="ACHURS_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFR1236767.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8621..10159)
                     /gene="narH"
                     /locus_tag="ACHURS_000976"
     CDS             complement(8621..10159)
                     /gene="narH"
                     /locus_tag="ACHURS_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFR1236768.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10156..13899)
                     /locus_tag="ACHURS_000977"
     CDS             complement(10156..13899)
                     /locus_tag="ACHURS_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFR1236769.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVSTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFLDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14292..15683)
                     /gene="narK"
                     /locus_tag="ACHURS_000978"
     CDS             complement(14292..15683)
                     /gene="narK"
                     /locus_tag="ACHURS_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFR1236770.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16022..17818
                     /gene="narX"
                     /locus_tag="ACHURS_000979"
     CDS             16022..17818
                     /gene="narX"
                     /locus_tag="ACHURS_000979"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFR1236771.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDEQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLLDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17811..18461
                     /gene="narL"
                     /locus_tag="ACHURS_000980"
     CDS             17811..18461
                     /gene="narL"
                     /locus_tag="ACHURS_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFR1236772.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18462..19856)
                     /gene="ychO"
                     /locus_tag="ACHURS_000981"
                     /gene_synonym="iatA"
     CDS             complement(18462..19856)
                     /gene="ychO"
                     /locus_tag="ACHURS_000981"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFR1236773.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVRVEQYFGERVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20041..20394
                     /gene="ychN"
                     /locus_tag="ACHURS_000982"
     CDS             20041..20394
                     /gene="ychN"
                     /locus_tag="ACHURS_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFR1236774.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20438..21133)
                     /gene="chaC"
                     /locus_tag="ACHURS_000983"
     CDS             complement(20438..21133)
                     /gene="chaC"
                     /locus_tag="ACHURS_000983"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFR1236775.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPNE
                     GPVWIFGYGSLMWNPALEFTELCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21291..21521)
                     /gene="chaB"
                     /locus_tag="ACHURS_000984"
     CDS             complement(21291..21521)
                     /gene="chaB"
                     /locus_tag="ACHURS_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFR1236776.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21791..22891
                     /gene="chaA"
                     /locus_tag="ACHURS_000985"
     CDS             21791..22891
                     /gene="chaA"
                     /locus_tag="ACHURS_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFR1236777.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23294..23401
                     /locus_tag="ACHURS_000986"
     CDS             23294..23401
                     /locus_tag="ACHURS_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR1236778.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVSWWRNRK"
     gene            23829..23936
                     /locus_tag="ACHURS_000987"
     CDS             23829..23936
                     /locus_tag="ACHURS_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR1236779.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(24085..24939)
                     /gene="kdsA"
                     /locus_tag="ACHURS_000988"
     CDS             complement(24085..24939)
                     /gene="kdsA"
                     /locus_tag="ACHURS_000988"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFR1236780.1"
                     /translation="MKQKVVSIGDINVANDLSFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(24975..25784)
                     /gene="sirB1"
                     /locus_tag="ACHURS_000989"
     CDS             complement(24975..25784)
                     /gene="sirB1"
                     /locus_tag="ACHURS_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFR1236781.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(25788..26180)
                     /gene="sirB2"
                     /locus_tag="ACHURS_000990"
     CDS             complement(25788..26180)
                     /gene="sirB2"
                     /locus_tag="ACHURS_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFR1236782.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(26177..27010)
                     /gene="prmC"
                     /locus_tag="ACHURS_000991"
     CDS             complement(26177..27010)
                     /gene="prmC"
                     /locus_tag="ACHURS_000991"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFR1236783.1"
                     /translation="MEYQYWLREAISQLQASESPRRDAEILLEYVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(27010..28092)
                     /gene="prfA"
                     /locus_tag="ACHURS_000992"
     CDS             complement(27010..28092)
                     /gene="prfA"
                     /locus_tag="ACHURS_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFR1236784.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(28134..29390)
                     /gene="hemA"
                     /locus_tag="ACHURS_000993"
     CDS             complement(28134..29390)
                     /gene="hemA"
                     /locus_tag="ACHURS_000993"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFR1236785.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            29604..30227
                     /gene="lolB"
                     /locus_tag="ACHURS_000994"
     CDS             29604..30227
                     /gene="lolB"
                     /locus_tag="ACHURS_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFR1236786.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            30227..31078
                     /gene="ispE"
                     /locus_tag="ACHURS_000995"
     CDS             30227..31078
                     /gene="ispE"
                     /locus_tag="ACHURS_000995"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFR1236787.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            31229..32176
                     /gene="prs"
                     /locus_tag="ACHURS_000996"
     CDS             31229..32176
                     /gene="prs"
                     /locus_tag="ACHURS_000996"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFR1236788.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            32300..33979
                     /gene="dauA"
                     /locus_tag="ACHURS_000997"
     CDS             32300..33979
                     /gene="dauA"
                     /locus_tag="ACHURS_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFR1236789.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(34034..34312)
                     /gene="ychH"
                     /locus_tag="ACHURS_000998"
     CDS             complement(34034..34312)
                     /gene="ychH"
                     /locus_tag="ACHURS_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFR1236790.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            34589..35173
                     /gene="pth"
                     /locus_tag="ACHURS_000999"
     CDS             34589..35173
                     /gene="pth"
                     /locus_tag="ACHURS_000999"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFR1236791.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            35290..36381
                     /gene="ychF"
                     /locus_tag="ACHURS_001000"
     CDS             35290..36381
                     /gene="ychF"
                     /locus_tag="ACHURS_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFR1236792.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRVGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
BASE COUNT         9264 a         9664 c         9094 g         9252 t
ORIGIN      
        1 tctggggccg tttttttgtt tacccaaagc aacttttcca taaaccgaca gcattagcct
       61 tcatcatatt tgcgacgatg tataacgcct aaacacaggg atattgtact ttacaggtca
      121 caagtcaacg tcggtgctta agagccctgt gaggcgtata gcggcgttaa aaaactgccg
      181 agaagggtat atagcccgga agaagtgcgt aaaacgaact gacaggataa aagtgccctg
      241 ctcaccctgt cagtaaagaa attcttatta atcgtggcga tgcctttcct gaatagccgt
      301 taatgagccg acttgtaacg cctctttata gtgtttacgg catacagaaa cgtatcgttc
      361 attaccacca attaccacct gctcaccttc gttataaggt ctgcctgctt gatcaagacg
      421 cagcaccatg cttgctttac ggccacaaaa acagatggtt tttaattcaa ccagtttgtc
      481 ggaccatgcc agtaagtatt ggctgccaat aaataattca cctcgaaaat cggtacgtaa
      541 accataacaa agtacgggta tatcgagttg atcgacaacc tccgataatt catatacttg
      601 ttgtctggtt aaaaactggc attcatcaac cagtacgcaa tgaattgcct gctgttcatg
      661 ttccgcacga atctcatcaa ataatgatga attttggtta aataattttg caggcgatga
      721 caaacctata cgcgaactga ctttcccggc accaaagcga tcatcaattt ctgccgtata
      781 tacgacagtg cgcatgccgc gttcctggta attgtatgaa gattgcaaca atgctgtaga
      841 cttacccgca ttcattgcgg aatagtagaa atatagctgt gccatcagcc acaggccctc
      901 aatgatatga aataaagttg gctggagttt atcataattc gtcagttttt cagctcaaca
      961 gtatgcacag aagtgggtat atgcgttctc ccttacgaag ccttgcataa tccttctgag
     1021 ctatcattac aactgcctcg cttgttataa gcgggtaaat gactactggt aactattcac
     1081 aatctttaac ctgttgcgca agtaatagcc ctctgttgac ctccaggaga tagtgcaata
     1141 ctaagtccat gctcttattg cgactgttct acttttcatc attcgcttaa tagggaattc
     1201 tcgtaaacac aactaataca gaagactgaa aggtcgtcag cctacgataa tctccccata
     1261 aaatgtgaca tgaatcagga agttttaacc tcacgtgctg cgaaatcatc ggtgtaaata
     1321 gggctatatg ccgcgtcttt tctggctaat tttatgaaaa gatatttatt ggcggcacaa
     1381 aataaagaac aattttgaat tccttacatt cctggctatt gcacaactga atttaaggct
     1441 ctattattac ctcaacaaac caccccaata taagtttgag attactacaa tgagcgaagc
     1501 acttaaaatt ctgaacaaca tccgtactct tcgtgcgcag gcaagagaat gtacacttga
     1561 aacgctggaa gaaatgctgg aaaaattaga agttgttgtt aacgaacgtc gcgaagaaga
     1621 aagcgcggct gctgctgaag ttgaagagcg cactcgtaag ctgcagcaat atcgcgaaat
     1681 gctgatcgct gacggtattg acccgaacga actgctgaat agccttgctg ccgttaaatc
     1741 tggcaccaaa gctaaacgtg ctcagcgtcc ggcaaaatat agctacgttg acgaaaacgg
     1801 cgaaactaaa acctggactg gccaaggccg tactccagct gtaatcaaaa aagcaatgga
     1861 tgagcaaggt aaatccctcg acgatttcct gatcaagcaa taatcttttg tagattgcac
     1921 ttgcttaaaa tcccgccagc ggcgggattt tttattgtcc ggtttaagac aatttaataa
     1981 gaataagcta taaaaaaacg gcgtcgattg ctcaacgccg tttcgtggat aacaccgata
     2041 cggatgttac ttcttaatgc ccatctcttc ttcaagccag gctttaaatt ccgtgccaag
     2101 ggtgttatga cgaataccgt attcaacgaa ggcctgcatg taacctaatt tattaccgca
     2161 gtcatggctc ttccctttca tatgataggc ttccaccgtt tctttttcga tcagcatatc
     2221 aattgcgtcg gtgagctgaa tttcatcacc agctcccgga ggggtttttg ccagcaacgg
     2281 ccaaatatcc gcgctaagta cgtaacgacc cacaatagcg agattagacg gcgcaacatc
     2341 cgctttcggt ttttctacca caccaaccat cggtacgctt tcacccggcg ctaattcaac
     2401 gcctttgcaa tccacaacgc catatgcggt cacatcagca accggttcaa ccatgatctg
     2461 gctatgaccc gtttcatcaa agcggcggat catctctgcc aggttatcct gtgacaaatc
     2521 ggattcatat tcatccagaa taacatcagg caaaataaca gctaccggtt catcacccac
     2581 taccgggtga gcacacaata ccgcgtgtcc caggcctttc gccagaccct gacgaacttg
     2641 cataatagtc acgtgcggtg gacaaataga ctgcacttca tcaagcagtt gacgttttac
     2701 acgtttttcc agcattgctt ccagttcaaa actggtatca aagtggtttt caatagagtt
     2761 tttagatgag tgtgtaacca gcacaatttc agtaatgcca gccgcaatac attcattcac
     2821 gacgtattga attaatggct tatcgacaag tggcagcatc tctttcggga tggctttcgt
     2881 cgccggcaac atcctggttc ctaatcccgc aacggggata acggcttttt tgactttcgt
     2941 attaatggca gccatttaaa ttctcctgga ctgttcgtgt tttgaacgtg ttcatatttc
     3001 tgtatcgcat cccagtatat cagcaccccg aaggggctaa gttctgaaaa ggaggcgagt
     3061 tagcataatt aagacaatta cttatacgaa tggcgacgat agtaccacca gccgacatta
     3121 gcaggtaatg caaatttagc ccgcgttatc gtttgctcat tctgcagaca acatcaagcg
     3181 cagtcgacca ccggttcccc atatttggca ttgccaggca tcgcatcgct ggctcaattg
     3241 attcaaataa gcgttaccta aagtgcctaa cggaacacca ttactgattt gcacctggtg
     3301 ttcgccggta tttaacgtcg cattcagacc cgcagaaacc agaatgagat ttttcagttc
     3361 gcgatgataa tagccaacta atagcggaaa ctgccccggc agattggcct gacggagtaa
     3421 atggtttacc tgcttcaata acgcgcccaa ctctggcaac cgttgatttt ggtgtgcaag
     3481 ctgttcctgt aataatccgt taaacaatgc gcgtaataac aaggcagcaa gtacgccatt
     3541 atgtccagct cgggtgacat caaggcaata aaatgccaga tcgttttccg aaagtgcggc
     3601 aatatcaagc accaggccgg gtttgtccgc ggcaaccaat tgacgataat taacccggca
     3661 atgggaaatc acctgctgaa ccggcggttg tagttcctgt aataatttcg ccgctgcggc
     3721 agggttatca accattgcat cccagtcgcg aaaaagcctt tcctcttcct caacgcgcga
     3781 attaaacatg ctgggataga gacaggcaaa aaccatctcg cgcaagcgat tcagatcttt
     3841 aactggtttc agcaaaacat cttcaacgcc cagacgtaac gctttggcaa tatctgccat
     3901 attttcagtg gcagatatca ccagaactgg ggtctggtcg cctctgttac gtatatgctc
     3961 cagcagttta agcccgttca ttcgtggcat cgcgatatca catatcatca ggtctggagt
     4021 gaaacctccc agcaactcaa gggcatccac cccatcagcc gccagtaccg ttgtcgctcc
     4081 caatgaggaa aaccatgaat ccagaagcga gcgaaatacc tgctcatctt caacaatgag
     4141 aatctgtttt ccgaccaatg gctgcgtcat gttctctccc ctgactggct ttactcaata
     4201 gtggcatgct attgccacct gcgcctgtca gaattaactt aagtgtagat aaaaattctg
     4261 gtcaaatgtt ggtgcgtacc aacggcaaaa gttcgtccat tttcctttcc actgccagct
     4321 gtccggccgc aatggcagcg tgcgcgcgat ggaaatcaag cgtagatatt tgcgggcaaa
     4381 caggttgaat cagaatatcg ggcggatcac ctgccatgcg gttcctttta aggcggttct
     4441 ccaacacctg gatagacgtg gtcataatct ctgttgccgt tggcgctgtc acagcccgac
     4501 gtgtcgttat gctgcctaac ctttctttca gacgcgcatg ccacggtaga gaatcaccat
     4561 tctcgctatt ttcttcactg acattaaagg agagcagatc ttgttgcatc aaatgagcat
     4621 cgtgctgcag gtcgaccgct atcacaatat cagcccccaa tgcacgcgta agagaaatag
     4681 gaattgggtt aactactgcc ccatcaacca gccagtagcc gttatgtgca acgggtgcca
     4741 tgagtcctgg aatactgcat gatgcgcgaa tagcaagatg gagatcgcct tcagtaaacc
     4801 ataattcacg ccccgtactt aaattggtgg caacagccgc aaagcgacgg gaacaatttt
     4861 cgatctctgt ttccggcatt atttcgcgat attgattgaa gacacgctcg ccgcgcagta
     4921 acccaccgcg ctgccaggag aggtccatca ggcgtaaaac gtcccaataa ctgaaagagg
     4981 ttacccaatc ttccagcgca gataatcgat cgcatgcata ggccgcgccc accagcgaac
     5041 caattgaaca tcctgcaacg atatcaattt caatacccac tttttttagc gcattaataa
     5101 cgccaatatg cgaccaacct ctcgccgcgc cagatcccag cgccagccct atttttatct
     5161 ttctcattat tcccgcaaga ttcccctgga atgcaatcgt agccacatcg ctactgctcg
     5221 gttaacatag tgctactcag gcggttaata cgccgttatt gtttccaggg agattatttg
     5281 tgtctcagct ttgtccctgt ggtagtgctg tcgagtatag cctatgttgc cacccttatg
     5341 tgtctggtga aaaggttgca cctgatccag aacatctcat gcgttcgcgt tactgcgctt
     5401 ttgtgatgca agacgcagat tatttaataa agacctggca tccctcttgt ggagcagcgg
     5461 cgttacgtgc cgaattgatg gccggatttg cgcatactga atggttggga ttgaccgtat
     5521 ttgaacattg ttggcaggac gccgacaata tcggatttgt cagttttgtt gctcgattta
     5581 ccgaaggtgg caaaacaggt gcaataattg aacgttcgcg atttttgaaa gaaaacggtc
     5641 aatggtacta tattgacggt acacgtccgc agtttggtcg caacgatccc tgcccttgtg
     5701 gttcaggtaa aaaatttaaa aagtgctgcg gccaataatg gttgacggta cggtttagca
     5761 aacactctca acaaggtttt tccagcaatg cattcactcc aacgtaaagt tctgcgtact
     5821 atttgtccgg accaaaaagg tctgatcgca cgtattacca atatttgcta caagcacgag
     5881 ttaaatatcg tacagaacaa tgaatttgtt gatcaccgta ccgggcgctt ttttatgcgc
     5941 acggaactgg aagggatttt taatgattcc accctgctgg cggatctcga tagcgcattg
     6001 ccagaaggct ccgtgcgtga gctgaatcct gccggtcgtc gccggatagt gattctggtc
     6061 actaaagaag cgcattgcct tggcgatttg ttgatgaaag ccaattacgg cggcctggat
     6121 gtcgaaatcg cggcagttat tggtaaccac gatactttac gttctctggt tgagcgtttt
     6181 gatattccgt ttgagctggt aagccatgaa gggttaagcc gcaacgagca cgatcaaaag
     6241 atggcggaag ccattgatgc ttatcaacct gactatgtgg tgctggcgaa gtatatgcgg
     6301 gtattaacgc cggaatttgt ggcacgcttc ccgaataaaa tcatcaatat tcaccattca
     6361 ttcctgcctg cgtttatcgg cgcacgtcct tatcaccagg cctatgaacg tggtgtgaag
     6421 attattggcg caaccgctca ctatgtgaat gacaatctgg acgaaggccc aatcatcatg
     6481 caggacgtta ttcatgtcga tcatacctac acagctgaag atatgatgcg cgcaggtcgt
     6541 gacgtcgaga aaaacgtctt aagtcgtgcg ctatacaaag tactggcaca gcgcgtcttt
     6601 gtttacggta atcggacgat tattctttaa tcgccagcaa aaataactgg ttacctttaa
     6661 tccgttacgg atgaaaatta cgcaaccagt tcatttttct caacgtaaca ctttacagcg
     6721 gcgcgtcatt tgatatgatg cgccccgctt cccgataagg gagcaggcca gtaaaaagca
     6781 ttaccccgtg gtggggttcc cgagcggcca aagggagcag actgtaaatc tgccgtcatc
     6841 gacttcgaag gttcgaatcc ttcccccacc accatcactt ttcaaaagtc cgtaaactct
     6901 caaacgaatc cgcaattaaa tattctgccc aagcggggaa ggatgagaag cttcgaccaa
     6961 ggttcgactt gagcgccagc gagagagcgt tgccgcaggc aacgacccga agggcgaagc
     7021 gcgcagcgct gagtaatcct tcccccacca ccatcgcttc cttaaataat caaaacctgt
     7081 tactccgagc taattttgcc cgatggcacc acgtttaccg ggcctacaat atcctcccct
     7141 gtaggtcgga taagcgaagc gcatccgaca actcaccccg caccgtgccc ataaaaaaac
     7201 cccgccgaag cagggtctat gtgaactaaa attcgcttag tgacgagcgc gcaccagctg
     7261 gtacttacgt gtcagatact ccaccggtac gctccagatg tgtaccagac gcgagaacgg
     7321 gaacagcagg aataacgtca tccccagcac caggtgcaga cggaagataa acgccacacc
     7381 atcgaggtgt tgagaagcgc caccgtggaa ggtcaccacc gactgcgccc agccaaccag
     7441 tttcatcatc tcgctaccgt ccatatgctg agcggagaac ggaatggtca acaggcccag
     7501 cgcgcactgg ataacgagca gcgacaggat caggatatcc gctccggtag tggttgcacg
     7561 cacgcgtggg ctgaacagac gacgcttcag caacagcacg ccgccaatca gacacagcac
     7621 gccgctggca ccaccagcaa acatcgccat tttctgtttc acttcaatcg gcagccaggc
     7681 ttcatacatc cagtgcggcg tcagcatacc gaagaagtga ccaacaaaaa tccccagaat
     7741 accgatatgg aacaggttcg acgccaggtt catccctttg cgatccagca tctggctgga
     7801 cgccgcgcgc caggtgtact gcccgtagtc ataacgcagc cagctaccaa tcaggaagac
     7861 cgccccggct atgtacgggt agatatcaaa gaagaacata ttcaggaatt gcattagtgc
     7921 tgtcctccgg tggtgatatt cagatattgc ggcgcgacgg caccggcaaa gcgacgctga
     7981 tgagcagtga ttgctgaatc accgcagcct ttgtcagcaa agaatttaac ctgctcttct
     8041 tcccagacag catccagcgc ctgcggcgta tcatcgcgcg cttcgtcggc aattttttcc
     8101 gccactttgt cgctgtcgat agcggtattg gccaatttca gcagcagatc aaacatcacg
     8161 gcataacggc tttcacgctg ttgcagacgc gcgctcagca atgccagaat cggcgcgata
     8221 tctttcaaac cttccacggc ttcgctttgc ggcagctgcg acaggtactc cagatacagc
     8281 ggcagatggt ccggtaattc gcggctgttt aactgcaagc cgtgctgctc gtactgcgcc
     8341 agcaggtcca ccatcgcctg gccgcggtca cgggattcac cgtgaacatg ttcaaatagc
     8401 aacagtgacg tggcgcggcc acggtcgaac agttcgctgt actgggcctg ggcatcgagc
     8461 ggatccatcg ccgttaaatc gcgcaggaaa atgcccagcg catgggcatc ttctttcgac
     8521 agatttttcg acgcagcaat cgcctcaaac atctcttgtt gatgctgcca taaggcagca
     8581 tccggatatt caaggagacg cgatacaatc acgagttcga tcatggatgc ggctccgttt
     8641 tgctggtcac atcgatggca tcaatacgac ggctgttgaa cagattgaat ttggtatctg
     8701 aaccgtggca gccatcacca aaggtaaagc cgcagccatt tttctccggg aaggcttccc
     8761 gtgccagttc acgatgacta ctcggcacca caaagcgatc ttcgtagtta gcaatcgcca
     8821 gataacggta catctcctgt gcctgggctt cggtcagacc gacctcttcc agcgcacggg
     8881 tatcaacttt accgtcaacg gtttcagcac gtttgtaatg acgcatcgcc agcatacgtt
     8941 tcagtgcgcg cagtaccggt ttggtatcac cggcggtcag cagattcgcc agatactgta
     9001 ccggaatacg caagctttcg acgtctggca gaatgccgtt gctacccagc tcacccgcgt
     9061 ctgctgcaga ctgaatcgga gacagaggcg gcacgtacca gaccatcggc agtgtgcgat
     9121 attctggatg cagcggcagc gccagtttcc attccattgc cattttataa accggcgact
     9181 gctgtgcggc ttcaataacg ctcagcggaa taccgtcttt aatcgcctgc tcgatgactt
     9241 tcggatcgtt cggatcgagg aacacctcca gctgacgctg gtaaagatct ttctcgttct
     9301 cggtgctggc tgcacgttca atcgcgtcgg catcgtacag cagcacgcca agataacgga
     9361 tacgaccgac acaggtttct gagcacacgg tcggctgacc cgcttcaata cgcggatagc
     9421 agaagatgca cttctcagac ttaccgctct tccagttgaa gtagattttt ttgtacgggc
     9481 atccggtgat gcacatacgc cagccacggc atttatcctg gtcgatcagg acgatgccat
     9541 cttcttcacg cttgtaaatc gcaccgctcg ggcaggtcgc cacacatgcc gggttcaggc
     9601 agtgttcgca caggcgcggc aaatacatca taaaggtgtt ttcgaactgg ctatacatcg
     9661 ccttctggat gttgtcgaag ttcttgtctt tcgccagttt gtcaaactca ccacccaggt
     9721 catcttccca gttcggccct ttttcgattt tcgccatccg ttccccggta atcagcgaac
     9781 gcggacgggc aatcggctgc gatttgctgc cttccggcgc ggtatgcagg ttctgatagt
     9841 caaaatcgaa cggctcgtaa taatcgtcga tccccggcag atgcgggtta gcgaagattt
     9901 tacccagcaa catggcacgg ttacccatgc gcggctgcag tttgccgttg attttacgga
     9961 tccagccgcc tttgtatttt tcctggtttt cccagtcagt cgggaagccc tgacccggct
    10021 tggtttccac gttgttgaac cacgcgtatt ccacgccttc acggctggtc cagacgtttt
    10081 tacaggtaac tgaacaggtg tggcagccga tgcacttatc gagattcagc accatgccga
    10141 cttgtgaacg aattttcatt ttacgctctc ctgtacctgg tcattgcctt cgccatctaa
    10201 ccagtcaatg ttcttcatct tacgcactac aacaaactca tcgcggttag aacctacggt
    10261 gccgtagtag ttaaagccgt atgccagatg ggcatagccg ccgatcatat gcgtcggttt
    10321 cggcgtgata cgggtgaccg agttatggat accaccacgc tgttgggtaa tttccgaacc
    10381 aggcaggtta acgatacgtt cctgcgcgtg gtacatcatg gtcatccctg ccggaacacg
    10441 ctggctgaca accgcacggg cagtcagagc accgttgctg ttgaatactt caatccagtc
    10501 gttatcggcg atacccagat ctttggcatc ggcttcactc aaccagacca ccggaccacc
    10561 gcgacctaaa gtcagcatca gcaggttgtc gctgtaggtg gagtggatac cccacttctg
    10621 gtgcggcgtc aggaagttga gcgctttttc cgggttgccg ttggatttct ggcctatcac
    10681 ttctttcacc gaacgggtgt cgatcggcgg acgataaacc agcaggcttt caccgaaatc
    10741 acgcatccac tggtgatcct gatacagttg ctgacgacca gagagcgtac gccatgggat
    10801 cagctcgtga acgttggtgt aaccggcgtt gtaagaaacg tgttcatctt ccagaccaga
    10861 ccaggtcggg ctggagataa ttttgcgcgg ctgtgcctga atatcgagga agcggatctt
    10921 ctcgtcttct ttattcagcg ccagatgcgt atggtcacga ccggtaaatt cgctcagggc
    10981 agcccaggct ttcacggcta cctgaccgtt ggtttccggt gccagtgtca ggatcatctc
    11041 tgccgcatca attgcggtgt tcagcatcgg ctggcctttc gccggacctt ccgctttggt
    11101 gtagttgagc ttacgcagca gatccatctc gctctgggtg ttccaggcaa tccctttacc
    11161 gccattaccg attttctcca tcaacgggcc aatagaggta aagcgttcgt aagtcgccgg
    11221 ataatcgcgc tctacgacca taatgtgtgg cgcggtttta cctgggatca ggtcgcactc
    11281 gccttttttc cagtctttca catccagcgg ctgcgccagt tcagcggcag agtcatgctg
    11341 gataggcagc gtgacgatgt cggtttcttt acccagatgg ccgacgcaca cttcggagaa
    11401 tttcttcgcg atggctttgt agatttccca gtcgcttttc gcttcccagg ccggatcgac
    11461 cgccgcagac agcgggtgaa taaacggatg catatccgaa gtattcatgt cgtctttttc
    11521 gtaccaagtc gccgtcggca aaatgatgtc ggaatagaga caggtgctcg acagacggaa
    11581 gtccagcgta accaccagat ccagcttgcc ttccagacca ttgtcctgcc agtccacttc
    11641 ttccggcttc acgccgccct gttgccccag atctttaccc tggataccgt gctccgtccc
    11701 cagcaggtac ttgagcataa actcatgacc tttaccggaa gaaccgagca ggttagaacg
    11761 ccagatgaac aggttgcgcg ggtggttttt accgttttct ggttgttctg ccgcaaaacg
    11821 gatggaaccc tctttcaggg atttcaccgt atagtccacc ggattcatcc cggctttttc
    11881 cgcttcgcca gcgatagtca gcgggttagt gcctaactgc ggtgcagacg gcagccagcc
    11941 catgcgttcc gcacggacgt taaagtcgat caagtgtccg gtatagcggg atttgtccgc
    12001 catcggtgac agcaactctt ccgccgtgac ggtttcataa cgccactggc tggagtggtt
    12061 atagaaataa gaagtgctgt tcatgtgacg cgccggacgc tgccagtcaa gggcaaacgc
    12121 cagcggctgc cagccggttt gcggacgcag tttttcctga cctacatagt gcgcccagcc
    12181 gcccccgctc tgaccgacac agccgcagaa aatcagcatg ttgatcagac cacgatagtt
    12241 catatcgagg tgataccagt ggttcagccc cgcaccgacg ataatcatcg aacgaccgtg
    12301 cgttttatca gcgttatcgg caaattcacg ggcgatgcga ataatctggc tgcgagaaac
    12361 gccggtaatc tgctcggccc aggccggggt ataagctttc acatcgtcat agctggttgc
    12421 acagttaacg tcgttcaggc cacgttccag accgtagttt gccagcgtca gatcataaac
    12481 ggtgctcacc agggcggtgc tgccatcagc cagttgcagg cgtttcaccg gcagtttgtg
    12541 cagcagcacg ttttccagtt ccactttgtt gaagtgttcc gtgccgtcgc caccaaagta
    12601 cgggaagcct acctcagcga tctcatcctg gctacccagc aggctcagtt gcagctcggt
    12661 ttcttcgcca gttttgccgt cgcgctgctc aagattccat ttgcccttct cgccccagcg
    12721 gaagccaata gaaccgttcg gcgcaaccat ttcgccattg gtattaaagg cgacagtttt
    12781 ccattccgga ttgttttcct ggcccagcgc atcaaccaga tcagcagcgc gcagcatacg
    12841 acctgcagcg tagtaaccgt cgcgttcttc cagcatcacc agcatcggca tgtcggtgta
    12901 gcgacgcaca tagtcggtga aatactggct tgggttgtcg aggtggaatt cacgcagcat
    12961 tacgtggccc atcgccagcg ccattgccgc atcggtgccc tgtttcggtg ccagccacag
    13021 atcgcacagt ttggcgattt cagcgtagtc tggtgtgacg gcaacagttt tggtgccttt
    13081 gtaacgcact tcagtaaaga agtgagcatc cggggtacgc gtctgcggca cgtttgaacc
    13141 ccaggcgatg atgtagctgg agttatacca gtcagcagat tccggtacgt cagtttgctc
    13201 gccccaggtt tgcggagacg caggaggcag gtcgcagtac cagtcgtaga agcttaagca
    13261 agtaccgcca atcagcgaga gatagcgtgc acccgatgcg taagaaacca tcgacattgc
    13321 cggaattggc gagaaaccag caacacggtc cgggccgtag tttttgatgg tgtaaacgtt
    13381 agatgcggcg atcagttcgt tcacctcctg ccaggaagaa cgaacaaatc caccgcgtcc
    13441 acgcgcctgc ttaaagcttt tcgctttatc ggcgtcttca atgatagaag cccatgcctc
    13501 aaccggatcg ctatgcagcg ccttcgcttc acgccacatt ttcatcaggc gtttgcgcat
    13561 catcgggtat ttcaggcggt tggcactgta aagataccag gagtagctgg caccgcgcgg
    13621 gcagccgcga ggttcatggt ttggcagatc cggacgggta cgcggatagt cagtctgctg
    13681 ggtttcccag gtgaccagac cgtttttgac gtagattttc cagctgcagg agccggtgca
    13741 gtttaccccg tgggtagagc ggacgatttt gtcatgctgc caacgctggc gatatccatc
    13801 ctcccagtca cggttggtat tgagaagctg gccatgccca tcggcaaagg tttcaccctt
    13861 ctgtttgaag tagcgaaacc ggtccaggaa tttactcatc ggttttctcc tgtgggagcc
    13921 tgtcggccct ctgataaatc gacattgcta atgtttaagg gcaaggtaac gctctgaaac
    13981 agcgtgggaa ttgataacga tcaagagtga tggggatgaa aaataaagta aattcctaag
    14041 ataccccttt aggataatta acagccacca cataacagac tgtaaattaa gacgttttat
    14101 acctgtagtt atttaaacta ttaagatttt tatgcagatg ggtattaagg agtattcccc
    14161 atgggtaaca tattaatggc tcttaatgaa atccggcgaa ataagccata taatatgagt
    14221 atgttgtaac taaatgacgc ataaaaaaag cgcgacatca tgccgcgcaa aggataatca
    14281 aatattaaga tttatttttt agaatgccga ccatataccg cccaggtaat cactacgcag
    14341 gcgatataga aaatcaaaaa taccttcatt gcgccgactg gcgaacccgt taatgccagc
    14401 gagctaccaa acgctttcgg gataaagaag ccaccaatag cgccaatcgc agagatgaaa
    14461 cccagcgccg ctgccgtgtc ggttgccgct tcacgcatcg cacgttcgtc agaaccccct
    14521 tctgctttca cgcgatccat tgtcagttta cggaagatca ctgaaatcat ctggaaagtg
    14581 gaaccactac ccagcccagc tgtcaggaac agcgccagga agaccgcgaa gaacgccatg
    14641 aagcttccgc cctgcccgtc agtcggtaag gtcaggaaca gcaggccgct gaaaatcgcc
    14701 atcagaataa agttcaccag cgtgacacga gttccgccca gacggtcaga taatgcacca
    14761 cctgcagaac gcgccagcgc accaataaac ggcccgaaga aagcgtattg cagaatctga
    14821 acatccggga actgcgtttt tgacagcatc gcaaagcccg cggagaagcc gataaaggag
    14881 ccgaaggttg ccagatacag caggctcata atccacagat gaccccgttt gagtaccggc
    14941 aactgctcct tgatggaggc tttcgaggta gcaagatcgt tcatgccaaa ccacgccgca
    15001 atggtgaaga tggcaaggaa cggcacccat atccaggacg cattcgccag atacagctca
    15061 gtcccatccg gctgtttgac gccctggcta ccaaatactg cgaaaatcga cagtgatacc
    15121 accagcggag caaccaactg catgacgctg acgcccatgt ttcccagacc accattcaga
    15181 cccagcgcgc caccctgctt ctgtttcgga aagaagaagc tgatgtttgc catactggat
    15241 gcgaagttcg cgccagcaaa gccgcatagc agagagatga tgatgaagac gctataaggc
    15301 gtggaggtat cctgcacggc aaaacccagc cagacgcaag gaataatcag aataccggtg
    15361 ctgaacgccg tccagcgacg accaccgaag ataggaacca taaaggagta tggaacacgt
    15421 aataacgcgc cagaaaccga aggcagcgca gtcaacataa atagctgatc ggtcgtaaaa
    15481 ttaaaaccga ctttcggtaa gttcaccgca acagcgctga acaacatcca tacgcaaaac
    15541 gccagcagca gacagggaac ggaaatccac aggttacggc tggcaatacg ttgaccgcgt
    15601 tgttgccaga acgcaggatc ttccggtcgc caatctgtaa tgacagctcc agtagccctt
    15661 tcgggggcgg atgagtgact catagacacc tctgatactc gtttcgatgt ctgccacctt
    15721 agtgtctgta gctaaaggca atttgatgta aatcaaacga taagataact ttatctatca
    15781 ttgatattta tcattaccca tagtgagtac agtgatttca taaaaattat gagatttttc
    15841 acggtgctgt aaaatcccta cccttaccga tgtaaagcga ctaaccacac ggcaaataag
    15901 gagtaactct ttccgggtat gggtatactt cagccaatag ccgagaatac tgccattcca
    15961 gaatgtatcg tcacattcat taaggttatt gctcatttaa agcctgaagg aagaggtttg
    16021 catgcttaaa cgttgtctct ctccgctcac cctggttaat caggttgcgc ttattgtgtt
    16081 gctttctact gctattggac tggcagggat ggcggtttct ggctggctgg tgcaaggcgt
    16141 tcagggcagc gcccatgcga tcaacaaagc gggatcgctg cgcatgcaaa gttaccgtct
    16201 gttggcggca gtgccattaa gtgagaaaga caagccctta attaaagaga tggaacaaac
    16261 ggcatttagc gccgagttaa ctcgagcagc agaacgagac gaacaactgg cgcaattaca
    16321 gggtttacaa gattactggc gtaatgaact gatccctgca ctgatgcgtg cacaaaaccg
    16381 cgaaacggtg tcagcggatg tcagccagtt tgttgccggg cttgatcagc tggtatctgg
    16441 ttttgaccgc accacggaaa tgcgcattga gacagtggta ctggtccatc gggtaatggc
    16501 ggtatttatg gcacttttac tggtgttcac tattatctgg ttgcgggcgc gactgctaca
    16561 accgtggcgg caactgctgg caatggcgag tgccgtcagt catcgcgatt ttacccaacg
    16621 cgcgaacatc agcgggcgca acgaaatggc gatgcttgga actgcattga acaatatgtc
    16681 tgcagaactg gccgaaagtt atgccgtact tgagcagcgg gttcaggaga aaaccgccgg
    16741 gctggagcat aaaaatcaga tcctctcttt tttatggcag gctaaccgcc gtttgcattc
    16801 ccgcgccccg ctgtgtgaac gcctgtcacc tgtactcaac ggcttacaga atttaaccct
    16861 gctacgtgat atcgaattgc gggtgtatga cactgatgat gaagagaatc atcaggagtt
    16921 tacctgccag ccagatatga cttgtgatga taaaggctgc cagctctgcc cgcgcggcgt
    16981 attacccgtt ggtgatcgcg gcacgaccct gaagtggcgg ctggctgact ctcatacgca
    17041 gtacggtatt ttgctggcga ccctgccgca ggggcgtcat cttagccatg atcaacaaca
    17101 actgctggat accctggttg aacaactcac cgccacgctg gcgctggatc gccatcagga
    17161 acgtcagcaa cagttgatcg tgatggaaga gcgtgccacc attgcgcgcg aactgcatga
    17221 ttctattgcc caatctctct cttgcatgaa gatgcaggtg agttgtttac agatgcaggg
    17281 cgatgcgctg ccagaaagca gccgcgaact gttaagtcag atccgtaacg aactgaatgc
    17341 atcctgggcg cagttgcgtg aattgctcac cacattccgc ttgcagctca ccgagcctgg
    17401 attacgtccg gcgctggagg cgagttgcga agagtacagc gccaaatttg gcttcccggt
    17461 gaagctggat tatcaattgc cgcctcgcct ggtgccttcg catcaggcaa tccacttgtt
    17521 gcaaattgcc cgtgaggcat taagtaacgc cctcaaacat tcgcaagcga gtgaagtcgt
    17581 ggtgacggtg gcgcaaaacg ataatcaggt caaactgacc gtccaggata acggctgcgg
    17641 cgtgcctgaa aatgccatcc gcagcaatca ctacggcatg ataataatgc gcgaccgtgc
    17701 gcaaagttta cgaggcgatt gccgcgtccg ccgtcgtgaa tcaggtggca ccgaagtggt
    17761 ggtcaccttt attcccgaaa aaactttcac agacgtccaa ggagataccc atgagtaatc
    17821 aggaaccggc tactatcctg ctgattgacg atcacccgat gctgcgaact ggcgtaaaac
    17881 agcttatcag tatggcacca gatatcaccg tggttggcga agcgagtaat ggcgaacagg
    17941 gtattgaact ggcggaatct cttgatcccg atctgatcct gttagatctc aatatgcccg
    18001 gcatgaacgg tctggaaacg ctggataaac tgcgcgaaaa gtccctctca gggcgcattg
    18061 tggtattcag cgtctctaac catgaagaag atgtggtcac cgcactgaaa cgcggcgcgg
    18121 atggttatct gttaaaagat atggaaccgg aagatctgct gaaagcattg catcaggcag
    18181 ctgctggcga aatggtatta agcgaagcat taacgcctgt tctggccgcc agcttgcgcg
    18241 ctaaccgtgc cactactgag cgcgatgtta accagttaac cccacgcgag cgcgatattc
    18301 tcaagctgat tgcccagggt ttgccgaaca agatgattgc ccgccgcctg gatatcaccg
    18361 aaagcacagt aaaagtgcac gtcaagcaca tgctgaagaa aatgaagctc aagtctcgcg
    18421 tggaagcagc ggtatgggtg catcaggagc gcattttctg attacggttc ccagcgcagt
    18481 tcgtcgtttg acaatgcatc gaacggttcg acaagcgtta gcgtgatctc attggaggag
    18541 acacgctgcc cctggttatc ttccaccacc accgacaatc gccagtgatt gctcgcccct
    18601 tccccgttct gccagtcagg catgatcagc gtccagccct ccgcgctgtt ggcttgtgcg
    18661 cccggcgtca aacttaatat ctgcgtatcg ccctgccaaa tcagttgccg aataccgtaa
    18721 cgactgcgga tttgtaattt cagcggcact gtttcgccag gttttagatc ccacggcggt
    18781 gtcgccagaa acaccgttaa cgtttttcgc tgtcggtact caagagtcgg tagattattt
    18841 cgctgcggat tatcatagcg actaccacgt aacgactgac tttcggcaac ctcgcccgca
    18901 gaaagttgtt ttttgagcgg cacaccaaag cggtaattaa gattcagtcc gaggttattt
    18961 tgattttcgc cactttcacc ctgtttatgc tgggccgtca cagtgactaa tggcacaggg
    19021 gtgtaattta atcccagact caacgcgacg ggattgtgat aacccgtacc agagttaaac
    19081 aaatcaacac gctcaccaaa atactgttct accctgacac tggtattgag gtgttgatag
    19141 aacggcatgc gcatccgggc tgtcaggtca tacccgcgcg ccatccgttg ttcctgcgtg
    19201 gctgtctgtt catgccatgc agcaaacggc tgataaaagt ttgccgatag tcgcaaatat
    19261 tcgccccacg cttcggcacc aaagcccgct cgctgaagat tttcgtccag caagttgtca
    19321 taaaaagtgt tataacccac cagccagttg ccgcgcgccc agcgttgccc aacgcccaca
    19381 ttgctcacca acccatcatc ctgctgagta agaccaagct ggctccaggt gagataacga
    19441 tcattatctt gtaacggcac aaaccagctt ccacgactgc cggtgaaatg tccttcgtta
    19501 tccactttga cgtcaacact ggcatttccc cacggtgata gccaggactc tacgtgctga
    19561 ttaacctgtt gactaagcgc gtcgcggact tttcccaatg cgaaagcttt tgcctgctcg
    19621 ccagtatcca gcccgttatc attcatactg gtttcgccga aatctttcac aatttcagca
    19681 aagtgttttt ccccatcatg gttttcggga gccatgccta aatccggcag accatcattg
    19741 ttattatcaa agggatttgc cgctttttgc tcgaaggtag attgtgcgtt agctgtaccg
    19801 cctgccgcca gcaagagtaa ataaaacggg ataatgcgag gaacgaaacg gctcaaaacg
    19861 tcggtaactt ccggtaataa tgaaaaacca tagccttaaa gaataacgat tactggcaac
    19921 ttttgtcgta ttttctcgcg ctttcaggaa aatccttaaa aaggtgaccg attggtcaga
    19981 cgctgcagaa gcagtaagat agaagatgag tgtctaattc cctgaataca ggagtaaata
    20041 atgcaaaaaa tcgtgatcgt tgccaatggc gcaccttacg ggagcgaatc cttgtttaac
    20101 agcttgcggc tggccattgc gttacgagag caggagagca atctggatct gcgtctgttc
    20161 ctgatgtctg atgcggtcac agccgggttg cgcgggcaaa aaccagggga aggctacaac
    20221 attcagcaaa tgctggagat ccttaccgct cagaatgtac cggtgaaatt gtgcaaaacc
    20281 tgtaccgacg ggcgcgggat tagcacactt cctcttattg atggggtgga aatcggtact
    20341 cttgtggaac tggcgcaatg gacgctgtca gccgataaag tgctcacatt ttaatgtttg
    20401 agggggcgtc tgacgcccca ctatgaggga agtttactca ggcgaatccc ggccgtaaca
    20461 caccatccgg ataattctcc gccagcagtt tttttaccga taccagcaaa tcattcagcc
    20521 catcgtcctg cattcccagt ttgataagct cctgttccag tgaaaacagg tattgtgcgt
    20581 tggttcccag cggaccgctc gccgccgcaa tcaacggcgc gatgacctga gcgcgagtgt
    20641 cagattcata ttctggatgt cgcgggtcca taataaacac aatggcgttt actgtgcgtc
    20701 catcatcaag atcaagctga caccaggttg gcagataaca gccggtaatc atctcgcgct
    20761 tccacaacag ggttagttcc tgctccagcg tctcttctgg tagtcgatag gcgacgcctg
    20821 tggtgcgtcc gccctctttc agtgcaagca tccgtcccgg ctggtgcgca gtcccgcgcc
    20881 cggcggtcag gcgcaggcaa aacgcgcgat gccatccaac cagtgtaccg gtgcataatt
    20941 cggtaaactc cagtgccgga ttccacatca acgatccata accgaagatc cacaccggtc
    21001 cctcattagg tcgacaagcc agcgtcgccg ccagcgaagc cgcccgttgt tctgctgacc
    21061 ataagagtga ttcttcaatg gcaccaaatg ccgttttaca atcggcattc atcaagaaat
    21121 cacgcgttat caccttacac ctccgccact gcatacttcc ctgcgacaac tttttggtgt
    21181 cttccggaca tttttaccgc tcattttgaa accactaacg ggacaatatg caatccacgg
    21241 accacttgca aggcaacacc gcacgaatag ctttaactaa gctaccggtt ttacgatttt
    21301 ttatgccatt tatcatcatc ccctttggca tattcatgct tcacagcagc ccaggccact
    21361 ttatgcgcgg tttcttcgcg actggcgtca tcacgccgat cttctttatc tttatattga
    21421 tcccatgcgc tgttgaacgc ttctttatag atatcctggg catgagacgg tagaacgtgc
    21481 tttacgcttt ccggcagatc gcttttcgtt ttatacggca tcgttgactc cttttacaaa
    21541 gttgaacatt aagtgtggtc aacaatatct actcttgcca aaacataaga attttcatgt
    21601 cagactgttc ttaagaaacg ctaaaacaaa cattgtctaa acgtaatact tatttttatg
    21661 ccccaatgag taaaaaatcg acagcggggt aaaaattcgt aaaatgttac cgatatccag
    21721 acactttctg ttatttttcc tacctatttt ttacattttc gataaattca tctgcaaagg
    21781 gataaccata atgtcaaatg ctcaagaggc ggtaaaaacc cgccacaagg agacttcgct
    21841 tattttcccg gttctggcgc tggtagtgct gttcctgtgg ggaagcagcc agacactacc
    21901 agtggtcatt gccatcaatc ttcttgcgct tattggtatt ttaagtagcg cctttagtgt
    21961 tgtccgtcat gcggacgtat tagcccatcg cctgggagaa ccttacggtt cgcttattct
    22021 tagcctttca gtggttattc ttgaagtcag tttgatttca gctttaatgg caaccggcga
    22081 cgccgcgcca acgctaatgc gtgatacgct ctattcaatc attatgattg ttaccggtgg
    22141 gctggttggc ttttcattat tgttgggcgg tcgtaagttt gccacccaat atatgaatct
    22201 gtttggtatc aagcagtatt taattgcgct gttccccctg gcgataatcg tactggtatt
    22261 tccaatggct ctgcctgcgg cgaatttttc aaccggtcag gcgttactgg tagcattaat
    22321 ttctgcggca atgtatggcg tatttttgct gatccagacc aaaacgcatc aaagtttgtt
    22381 tgtctacgag cacgaagatg acagtgatga cgacgacccg caccacggta aaccgtctgc
    22441 ccatagcagc gtgtggcatg ctatctggtt gattatccat ctgattgccg ttattgcagt
    22501 caccaaaatg aatgccagcc cgctggagac attgctcgac agcatgaatg cccctgtcgc
    22561 ctttactggc ttcctggtgg ctctgttgat tctgtcgccg gaaggtttag gtgcgttaaa
    22621 agcagtgttg aacaaccagg tccagcgcgc gatgaatctg ttctttggtt cagtgttagc
    22681 aaccatttcg cttaccgtac ctgtcgtcac gctaattgcc tttatgacgg gtaacgaatt
    22741 acagtttgca cttggtgcgc cagaaatggt ggtgatggtg gcctctttag tgctgtgcca
    22801 tatctccttc tccaccggac gtactaacgt gctcaatggc gcagcgcatc tggcactgtt
    22861 tgccgcctat ttgatgacga tatttgcctg acagttatat ctcctccgat gacggaaatc
    22921 gggggagata agataaattc ctgcttattg tatttcttca tactacctgg acagctcgtt
    22981 aatcagaatt gcgagcctgt accacaacct gcacgaaacc actgcaacag ctgttgaaaa
    23041 gccggaatgt actctttatt gcaaacattt cagggatgaa gtgaagatga attgagacgt
    23101 agtatccaca ccaagccgga attatccggt gaggcgcaat gttgcggggg ctttatccct
    23161 ggtggcattg gttgctggaa agagaaaacc cccgcacgtt gagaggtaaa acctgacaac
    23221 aaaacggggg ctaatcttga aaccagacac ttcaagaata gccgccttgt tgtcattaca
    23281 acaaggcggc tatatgacgc tcgcgcagtt tgccatgact ttctggcacg acctggcagc
    23341 accgatcctg gcggggatta ttaccgcagc gattgtcagc tggtggcgta accggaagta
    23401 aggtgaaaac gggcgtcagg ctgccgtgat ggcaatatgc gcccgaaacc ggccgcaggg
    23461 gtaacagcct gcggccattt ttgtctttat ctcctccaca agtaaatatt ggcaacccga
    23521 gctggaagca tcatccagag cactcatgaa acgactgtaa cagctgttga aaaaccggaa
    23581 tgcactcttc attgcaaaca tttcagggat gaagtgaaga tgaattgaga cgtagtatcc
    23641 acatcaagcc ggcgttatcc ggtgaggcgc aatgttgcgg gggctttatc cctggtggca
    23701 ttggttgctg gaaagagaaa acccccgcac gttgagaggt ttaacctgac aacagaacgg
    23761 gggctaatct tgaaccagaa caattcaaga atagccgcaa aatgttgtca ttacaacaag
    23821 gcggctatat gacgctcgcg cagtttgcca tgattttctg gcacgacctg gcagcaccga
    23881 tcctggcggg aattattacc gcagcgattg tcggctggtg gcgtaaccgg aagtaaggtg
    23941 aaaacgggcg tcaggctgcc gtgatggcaa tatgcgcccg aaaccggccg caggggtaac
    24001 agcctgcggc cttttttgta tctggcaccc atgaaaaaag tcttaacgca gaacgctaag
    24061 actttatttt tcaagcaaaa aagattactt gctggtatcc agttcttcga aacctttcac
    24121 cagatcatca atcgctttca tctgcttgag gaacggttcc agtttagcca gcggcagcgc
    24181 ggatggacca tcacatttcg catgttccgg atccggatgc gcttcaataa acagccccgc
    24241 cagacctacc gccataccgg ctcgtgccag ctcagccacc tgagcacgac gaccaccgga
    24301 agctgcgcca aacggatcgc ggcattgcag tgcgtgggtc acgtcgaaaa tcaccggcga
    24361 gttaccagac actttcttca taatgctgaa gcccagcata tcgacaacca ggttgtcata
    24421 gccgaagtta gcaccgcgat cgcaaagaat cactttttcg ttgccgcctt ctttgaattt
    24481 atcaacgata ttacccatct gtcccgggct gacaaactgt ggtttcttga cgttaattac
    24541 cgcaccggtt ttcgccatgg cttcaaccag gtcagtctgg cgagcaagaa acgccggcaa
    24601 ctgaatcaca tccacgacat cagcaacggg ctgtgcctga cttggttcgt gaacgtcggt
    24661 gataattttc acgccaaaag tctgcttcaa ctcctggaag attttcatcc cttcttccag
    24721 gcccggtcca cgataagagt ggatggagga gcggttggct ttgtcaaaag aggctttgaa
    24781 cacgtaaggg atacccagtt tctgggtcac ggttacgtag tgctcgcaaa tgcgcatcgc
    24841 cagatcgcga gattccagca cgttcatacc accaaacagt acgaacgaca ggtcatttgc
    24901 tacgttgatg tcgccaatgc taaccacttt ttgtttcata ggatcgcctt aatcttgagt
    24961 aaaatgtcga ttaattaatg cagcacaata tgtttatgcg cgatgttatt tatttgcgca
    25021 cggatcattt cgctgatcgg gtcttccgga cactgttcaa cgaaataact taaatcgttc
    25081 aacgcaacgt gttcgcaatc cagttgcgca taaatcaacc cgcgatcgcg aatttcatag
    25141 ggatcttccg ggttgaattg taataaagct tcgctggtgc gtaacgccag ctccatctga
    25201 ttttcttcca tcaacgaggc tttgagtgta tccaacaatt tgcggattac ctcaatgtta
    25261 tcagcttcat caaggtcttc ataaaacagt tccgccgacg ggctgatatt tccctttaac
    25321 catacgtcca gcatatgttc gcttaacgat tcaccgttaa aaggattaat cagccaaatt
    25381 tcgccatccg gacattcaat gcgcaatatc agctgcgtag ggaaaatcac cggcagcagc
    25441 ggcaaatcga gacgattcgc gacccataat aaaaccgcac ctaatgatac cgcactgccc
    25501 tgtcgattct ttaacacctg gtccagccat aatgcatcgg aaagacgata aacgccgcgt
    25561 gaggctttaa atccccagtc gccgtaaaac agcgcgatca gtttttccag ctgctcttct
    25621 aaaggcagaa gctggctgat ttcttccttc gccagactaa cgagacgctc cagttcgtcg
    25681 taaacatctt gcgagggaaa atcgcggcgg attgcttcgc aagccaggat catgccttcg
    25741 cacaatggcg ctttattaaa ttcgaaatca gctaacgatc tcatgactta ccccagtaac
    25801 ggtacttttg tggtggcgag tttaatgatg atgtacagca ccaccaacgc cagcgggaag
    25861 gcaataatgc gcgcctgctg actgtgcata cgacgataat cgagtgcaat aaaacccaaa
    25921 acgatataaa tgataactcc aaacagcttt tcagtcagcc actgtgcctg cccggaaaat
    25981 ggcaggatgt gcgctttagc catcaacgca atgccgctta acaataacac cgtatcgaca
    26041 accggcggca caatgcgcgt ccagcgagca aatgcctgtg gatgcttctg ataacgtagc
    26101 cagaaacgta aggttaatag cccaacagaa agcgcgatac taataagatg aacactaagc
    26161 agtgtagaaa aacttgtcat tgataatagc ggccgagcgt tacgcgctcg ttatcaccat
    26221 agtcacggca ggtttcgacg tcatgatacc ccgcgaggat aaatgcttgt cgcaccgctt
    26281 cgccctgctg ccagccatgt tccagaagca gaaagccgcc ggataccagc gcgttacgcg
    26341 actgttcgat gatatgcacg atgtctgcca ttccactgtc tgccgcaacc agcgcagtga
    26401 gcggctcaaa gcggacatcg ccttgttgaa gatgtgggtc ctgctcgtca atatacggcg
    26461 gattgctgac aatcatcgca aactgctgcc cggctagcgc gctaaaccag tcgctttgca
    26521 gaatgtggat atttttgatc gccagatgct gggcattacg ttgtgccagg gagacagcat
    26581 caggcatacg atctacagcg ataatttcgc agtccgggcg ctcgctagcc agcgcaagcg
    26641 caatcgcccc ggtacccgtc ccgagatcga gaatacggca aggttgttca ggcaaccgcg
    26701 ccagtgcctg ctccaccaga cactccgtat ccgggcgcgg aattaaggtc gctggcgaaa
    26761 caaataacgg caacgaccag aattctcgca ccccggttaa atgagcaatg ggttcaccat
    26821 cgcgacgacg tgtcagtagc gcatcaagtt gctgacattg ttcgtcagtc agctgcgttt
    26881 caccaaaggc cagaataaaa gtccgccccc tgccagtgac atactccaat aagatttcag
    26941 cgtcacggcg tgggctttcg ctcgcctgaa gttggcttat tgcttcacgt aaccagtatt
    27001 gatattccat tattcctgct cggacaacgc cgccagttgg tcggcctgat gttcctggat
    27061 aatcggttca atcagcatat ccagcttacc ttccatcact tcatccagac ggtagagcgt
    27121 caggttgatg cggtgatcgg taacgcgccc ctgcgggaag ttgtaagtac ggttacggtc
    27181 gctgcgatcg ccactcccca gcaggttacg acgggtagac gcttcggcct gttgccgttt
    27241 tgccatttct gcagcgtgga tacgagcacc aagtacagaa agtgctttag ctttgttttt
    27301 atgttgtgaa cgttcgtcct gacattcaac aacaatcccg gtcggcaagt gagtaatacg
    27361 aattgccgaa tcggtggtgt taacgtgctg accacccgcc cctgacgagc ggaaagtatc
    27421 aatgcgtaaa tctgctgggt tgatgtccgg cagttctgcg tcaggcagtt ctggcattac
    27481 cgcaacggta caagcagaag tatgaatacg accctgcgat tccgtagcag gaacacgctg
    27541 cacgcgatga ccgccggatt caaatttcag acgaccatac acaccatcac cgctaatttt
    27601 ggcgatgatc tctttataac caccatgttc accctcgctg gcgctcatga tttctacccg
    27661 ccagcggcgg gcttcggcat aacggctgta catacggaac agatcgcccg cgaacagcgc
    27721 cgcttcgtcg ccgccggttc cggctcggac ttcgaggaag gcgttacgtt cgtcatcagg
    27781 atcttttggc agtaacagaa cctgtaattg ctgttccagt tgctcgcttt tttctttagc
    27841 ttcgcgcagt tcatcctgcg ccatctcacg catttcagga tcatcgagca tcatctgtgc
    27901 ggtttcgata tcttcctgaa cctgttgcca gtcggtaaaa cagcgcgaaa catcacttaa
    27961 ctgcgcatat tcgcgtgata atgcgcgaaa acgttcctgg tcggcgatag tttgcgcgtc
    28021 acccagcaac gcctgaactt cttcatggcg ttcatgcagg gcttccagtt tggcaacgat
    28081 agaaggcttc ataggcgtaa atgcaccctg taaaaaaaga aaatgatgta ctgctactcc
    28141 agcccgaggc tgtcgcgcag aatattcagg cgttcgttat ccccgtcacg ggcggcctgt
    28201 tgaagtgatt tcgttggcgc atggatcaag cggttagtca gtttccatgc cagatcctgc
    28261 ataatggctt gcgcgtcgcc gccctgctca agggccgcta acgctttggc ggttaactca
    28321 tcgcgaacgt gctctgcctg gctgcgatac tcgcgaatgg tttcgctggc gctttgtgct
    28381 cgcagccacg ccataaattc gctggtttcc tgagcgacaa tagtttccgc ctcaaccgct
    28441 gcggctttac gctgcgccag gttgtgcgaa atgatgcttt gcagatcgtc cacactataa
    28501 agataagcat tcgccagttt gccaacttcc ggctcaacat cgcgcggaac ggcaatatcc
    28561 accaacagca ttggttggtt acgacgactt tttaatgcgc gctccaccat gcctttcccg
    28621 ataatcggta acgggctggc ggtggaactg atgatgatat cggcttcgcg cagacgttcg
    28681 tcgatctcac tcagggcaat cacttccgcg ccaacttcat ctgccagaat ttgggcacgt
    28741 tcgcgagtgc ggttggcgat aatcatcttc tgtactttat gttcgcgcag atgacgcgct
    28801 accagctcga tggtttcgcc cgcgcctacc agcaacactg tgaccgtaga gagcgattca
    28861 aagatctgcc gcgccagcgt acaagccgca aaagcgacag acacagcact ggcaccgata
    28921 tctgtttcag tgcgaacgcg tttcgctaca gagaaagatt tctggaacat gcgttccagt
    28981 tcgctggcct tcatatgacc tttttgcgaa tcggcaaacg cttttttaac ctgaccgagg
    29041 atctgcggct ccccaagaac cagtgaatcc aggccgctgg caacacgcat taaatggcta
    29101 accgcgtcgt tatcctgatg ccagtagagg cttttacgca gatcttcttc attaagattg
    29161 tgataatcgc aaagccagcg gattaacgcc tcttgcaggt tatcctgctc ttcaacgcta
    29221 agataaagtt ccgtgcggtt gcacgtcgac agcaccacgc cgccctgcac catcggctgc
    29281 gcaagcaggc tgtcaagcgc ctgatcgagc ttatccggcg aaaacgatac acgttctcgc
    29341 agcgatacag gtgccgtttt atggttgata ccgagtgcta aaagggtcat gtctgcggga
    29401 aataatacca acgttgatat ggttagtctg cttgcatcat acaggatgcg taagatcaat
    29461 aaaagagagc gccccctttt ggagtaattg ccgaaagccg ttagattctg gcaattaaga
    29521 caacttgaac atagacgata gcggacggta acgctagcat taagggttat aactgcaacg
    29581 tatctcaagg acttgtcatc actatgcccc tgcccgattt tcgtcttatc cgcctgctac
    29641 cgctggctgc tcttgtgctc actgcctgtt ccgttaccac gcccaaaggt cctggcaaaa
    29701 gcccggattc gccacaatgg cgtcagcatc agcaagacgt gcgcaatctt aatcagtatc
    29761 agactcgcgg cgcgttcgct tatatttctg accaacaaaa agtgtacgcc cgctttttct
    29821 ggcagcaaac cggccaggat cgctaccgtc tgctgctcac taacccattg ggcagcacgg
    29881 aactggagct gaatgctcaa ccgggtaacg tgcagttagt cgacaataaa ggtcagcgtt
    29941 ataccgccga tgacgccgaa gagatgattg gcaaattgac cggaatgcca attccgctca
    30001 acagcttgcg ccaatggatt ttaggtttac cgggtgatgc aaccgactac aaactggacg
    30061 accagtaccg cctgagcgaa attacctaca gccagaatgg caaaaactgg aaggttgttt
    30121 atggtggtta tgacaccaaa acgcaacctg cgatgccagc caatatggaa ctcaccgacg
    30181 gtggtcaacg catcaagtta aaaatggata actggatagt gaaataatgc ggacacagtg
    30241 gccctctccg gcaaaactta atctgttttt atacattacc ggtcagcgtg cggatggtta
    30301 ccacacgctg caaacgctgt ttcagtttct tgattacggc gacaccatca gcattgagct
    30361 tcgtgacgat ggggatattc gtctgttaac gcccgttgaa ggcgtggaac atgaagataa
    30421 cctgatcgtt cgcgcagcgc ggttgttgat gaaaactgcg gcagacagcg ggcgtcttcc
    30481 gacgggaagc ggtgcgaata tcagcattga caagcgtttg ccgatgggcg gcggtctcgg
    30541 cggtggttca tccaatgccg cgacggtcct ggtggcatta aatcatctct ggcaatgcgg
    30601 gctaagcatg gatgagctgg cggaaatggg gctgacgctg ggcgcagatg ttcctgtctt
    30661 tgttcggggg catgccgcgt ttgccgaagg cgttggtgaa atactaacgc cggtggatcc
    30721 gccagagaag tggtatctgg tggcgcaccc tggtgtaagt attccgacac cagtgatttt
    30781 taaagatcct gaactcccgc gcaatacgcc aaaaaggtca atagaaacgt tgctaaaatg
    30841 tgaattcagc aatgattgcg aggttatcgc aagaaaacgt tttcgcgagg ttgatgcggt
    30901 gctttcctgg ctgttagaat acgccccgtc gcgcctgact gggacagggg cctgtgtctt
    30961 tgctgaattt gatacagagt ctgaagcccg ccaggtgcta gagcaagccc cggaatggct
    31021 caatggcttt gtggcgaaag gtgttaatct ttccccattg cacagagcca tgctttaagc
    31081 cgggcaagct gagtttcggt gacaacgtca ccttgttcca gacgttgcat cgcgctcttt
    31141 aatacaccgc ctggaaagga tcatgcctgg cccgcacagt tttcggcaga ttctttccac
    31201 caatggacgc atgcctgagg ttcttctcgt gcctgatatg aagctttttg ctggtaacgc
    31261 caccccggaa ctagcacaac gtattgccaa ccgcctgtac acttcactcg gcgacgccgc
    31321 tgtaggtcgc tttagcgacg gcgaagtcag tgtacaaatc aacgaaaatg tacgcggtgg
    31381 tgatattttc atcatccagt ccacttgtgc ccctactaac gacaacctga tggaattagt
    31441 cgttatggtt gatgccctgc gtcgtgcttc cgcaggtcgt atcaccgctg ttatccccta
    31501 ctttggctat gcgcgccagg accgtcgcgt ccgttccgct cgtgtaccaa tcactgcgaa
    31561 agtggttgca gacttcctct ccagcgtcgg tgttgaccgt gtgctgacag tggatctgca
    31621 cgctgaacag attcagggtt tcttcgacgt tccggttgat aacgtatttg gtagcccgat
    31681 cctgctggaa gacatgctgc agctgaatct ggataaccca attgtggttt ctccggacat
    31741 cggcggcgtt gtgcgtgccc gcgctatcgc taagctgctg aacgataccg atatggcaat
    31801 catcgacaaa cgtcgtccgc gtgcgaacgt ttcccaggtg atgcatatca tcggtgacgt
    31861 tgcaggtcgt gactgcgtac tggtcgatga tatgatcgac actggcggta cgctgtgtaa
    31921 agctgctgaa gctctgaaag aacgtggtgc taaacgtgta tttgcgtacg cgactcaccc
    31981 gatcttctct ggcaacgcgg cgaacaacct gcgtaactct gtaattgatg aagtcgttgt
    32041 ctgcgatacc attccgctga gcgatgaaat caaatcactg ccgaacgtgc gtactctgac
    32101 cctgtcaggt atgctggccg aagcgattcg tcgtatcagc aacgaagaat cgatctctgc
    32161 catgttcgaa cactaatcga acccgctcaa agacccgctg cggcgggttt ttttgtctgt
    32221 aatatccatt tgtatgacct atgcctcctt cacctgccat ttagttgaca gatgatgcgc
    32281 tcacggatga aacattattg tgaacaaaat attttcctca catgtgatgc ctttccgcgc
    32341 tctgatcgac gcttgctgga aagaaaaata tactgccgca cggtttaccc gtgacctgat
    32401 tgccgggata accgtcggga ttattgctat cccgctggcg atggcgttgg ctattggtag
    32461 tggtgtggca ccccagtacg gtttatatac cgcagctgtt gcggggattg tcattgctct
    32521 gacgggtggg tcacgcttta gcgtttccgg tccgactgcg gcatttgtgg taattctcta
    32581 tcccgtgtcg caacagtttg gactggcagg actgctggtt gcgaccttgc tgtcggggat
    32641 ctttttgatt ctgatgggtc tggcacgctt tggtcgcctg attgagtata ttccggtttc
    32701 cgtcacctta ggtttcacct cgggtatcgg gatcaccatc ggtaccatgc agattaaaga
    32761 ttttctcggt ctgcaaatgg cccatgtccc ggaacattat ctacaaaaag tcggcgcatt
    32821 atttatggcg ctgccgacca ttaatgtggg tgatgctgcc attggcattg tgacgctagg
    32881 tattcttgtt ttttggccgc gtctgggcat tcgtttaccc ggtcaccttc cggccttgct
    32941 ggctggttgc gcggtgatgg ggattgttaa cctgctcggc ggacatgttg ctaccatcgg
    33001 ttcgcaattc cactacgtcc tggccgatgg ttctcagggt aacggtattc cgcaactgct
    33061 gccgcaactg gtgctgccgt gggatctgcc taattcagaa ttcacgctaa cctgggattc
    33121 tattcgcaca ctgctgcctg cggcattctc aatggcaatg ctcggcgcaa tcgaatctct
    33181 gctctgcgcc gtggtactgg atggtatgac cgggacgaaa cacaaggcga acagcgaact
    33241 ggttggacag ggactgggga atattatcgc tccgttcttt ggtggtatta ccgctacagc
    33301 tgccatcgcg cgttctgccg ctaacgtccg tgccggggca acttccccta tctcggcggt
    33361 gatccactct attctggtta ttcttgccct gctggtactg gcaccgctgc tctcctggct
    33421 gccgctttcc gctatggcag ccctgctgtt gatggtggcg tggaacatga gtgaagcgca
    33481 taaagtggtc gacttgctgc gtcatgcacc gaaagatgac atcattgtca tgctgctgtg
    33541 catgtcgctg accgtgctgt ttgatatggt tattgccatc agcgtgggga tcgtgctggc
    33601 atcgctgctg tttatgcgtc gtatcgcacg tatgactcgc ctggcaccgg tagtcgtaga
    33661 tgttccagac gatgttctgg tactgcgcgt tattggcccg ctgttttttg ctgctgctga
    33721 aggcttgttc acggacctgg agtcacgtct tgaaggcaaa cggattgtga ttctgaagtg
    33781 ggatgccgtt ccggtacttg atgctggtgg tcttgatgcg ttccagcgtt ttgtgaagcg
    33841 tctgcccgaa ggatgtgaac tgcgcgtgtg caacgtggaa ttccagccac tgcgcactat
    33901 ggctcgcgca ggcattcaac cgatcccggg acgcctcgcg ttcttcccga atcgtcgcgc
    33961 ggcgatggcg gatttataag tacgagattg accagtcagc gcaaactgac tggtcagcaa
    34021 actgcattat ttgttagcta tgacggcgat tatcgctacg gcggcaacgt ttgtcatagt
    34081 gacgaaccca ccagtaacgg tcgcacacct gttcatgccc gccaacacgg gcacccgcca
    34141 gccagagaat ggcaccgacg aaaatactta gcactgcacc atgtgcgaaa tactggggca
    34201 tattaaactg tggtaactgg ttgaggattg aataccccac gccgaccacc attaccacca
    34261 gccccaaccc catgagcacg ttaccgagta acgaagcgtt tttgcgtttc atatgtcacc
    34321 tccggaactt tctgggttgt aacagggaat acccctcttc cttatgtgta aagtatagac
    34381 aacaccctgt gggttatgtg cgggcgtgat cacaattaca acccttattt caacaaaact
    34441 ttacaaataa acgcctgaac accttgactt tctaatagtc cactcaggca attattacgt
    34501 tatctgcatt tgatacgcag ttttttgtca agcggggccg caaccagtaa actacgcgcc
    34561 agttatgtac acactcagga caaaaaacgt gacgattaaa ttgattgtcg gcctggcgaa
    34621 ccccggtgct gaatacgccg caacgcgaca taatgctggt gcctggttcg ttgacttact
    34681 ggcagagcgt ttgcgcgctc cgctgcgcga agaggctaaa ttctttggtt atacttcgcg
    34741 agtcactctt ggaggcgaag atgtccgcct gttagtcccg actacattta tgaatctcag
    34801 cggcaaagcc gttgcggcga tggccagttt tttccgcatt aatccggacg aaattctggt
    34861 ggcccacgac gaactggatc tgcctcctgg cgtcgccaaa tttaaattgg gcggtggcca
    34921 tggtggtcac aatggactga aagacatcat cagtaaattg ggtaataacc ctaactttca
    34981 ccgtttacgc atcggaatcg gtcatcctgg cgataaaaat aaagttgtcg gttttgtgtt
    35041 aggcaaaccg cctgttagtg aacagaagtt aattgatgaa gccatcgacg aagcggcgcg
    35101 ttgtactgaa atgtggttta cagatggctt gaccaaagca acgaaccgat tgcacgcctt
    35161 taaagcgcaa taagtcgttg tctgcggcat ttttgccgag tgccgtgtat aataggcaaa
    35221 gttatttcca tttctgcaat ctgttagcaa taacagattg attattaaga tattaaggtg
    35281 atttaaatca tgggattcaa atgcggtatc gtcggtttgc ccaacgtcgg gaaatctacc
    35341 ctgttcaacg cgctgaccaa agccggtatt gaagcggcca actttccatt ctgcaccatt
    35401 gagccgaaca caggcgtcgt accaatgcct gaccctcgcc tggatcaact ggctgaaatc
    35461 gtaaaaccgc aacgtacgct tcccacgacc atggaatttg tcgatatcgc cggtctggta
    35521 aaaggcgcat cgaaaggcga aggtctgggt aaccagttcc tgaccaacat ccgtgaaacc
    35581 gaagcgatcg gtcacgttgt tcgctgcttt gaaaatgaca acatcattca cgtttccggc
    35641 aaagttaacc cggctgacga tattgaagtt atcaacaccg aactggcgct ggcagacctc
    35701 gacacctgcg aacgtgcgat tcatcgcgta cagaagaaag ccaaaggtgg cgataaagac
    35761 gcgaaagctg agctggcggt cctagaaaaa tgcctgcccc agctggaaaa cgcaggtatg
    35821 ctgcgcgcgc tggatttaag cgctgaagag aaagcggcta ttcgttacct gagcttcctg
    35881 acgctgaaac caacaatgta catcgccaac gtcaacgaag acggttttga aaacaaccca
    35941 tatcttgacc aggtgcgtga aatcgcggcg aaagaaggtt ctgttgtggt tccggtttgt
    36001 gctgctgttg aagcagacat tgccgaactg gacgacgaag aacgtgacga gtttatgcag
    36061 gagcttgggc tggaagaacc gggcctgaac cgtgtgatcc gtgtcggtta taagctgctg
    36121 aacctgcaaa cttacttcac cgctggggtg aaagaagtgc gtgcatggac cattccggtt
    36181 ggagcaaccg cgccgcaggc agcgggcaaa atccatactg attttgaaaa aggctttatc
    36241 cgtgcacaaa ccatctcgtt tgaagatttc atcacttaca aaggtgaaca aggcgcgaaa
    36301 gaagcaggca aaatgcgtgc agaaggtaaa gattacatcg ttaaagatgg cgatgtgatg
    36361 aacttccttt tcaacgtcta attaatttat tgtctcatga ggtttcgcta aatctcatga
    36421 agagcgcaaa agtatataaa atccacgcaa ttgcgtggat ttttcatttc accgagtcct
    36481 aatcattctt gtaacatcct acacttttgt gcttattgcc tacagtgaac cacgagaata
    36541 attaaagaca tcagtgtcag atgacggata tctcattagt agtgatataa cgtaagtttt
    36601 tgttcaacta cacatcaccc ccttcacagc tgatcaatat tgatcacgct ccaatatgct
    36661 ggcgagaaaa ggacttacct gaagggtaat atcccgcaga taaagtcccc gaataacgtg
    36721 attacgtgcg atcctggcaa gatgaaaatg ataaccagta gcacaataac aacgataggc
    36781 cagaaaaccc attgaccaaa aatgaatatc gatgtttttt gctttttctt attagtaatg
    36841 caaaaatggc gcatattgtt tttgttatgc agatacatca agcgaaattc ccataactaa
    36901 ttaggattat tacaaaaaat cttcaccaaa ttttttaacc gctgtttgtt gtaatatttt
    36961 tgccatattt atagtaacga attggaataa gtaacgacaa cttagggaat aactcgatat
    37021 aaactcacct ttgtaagggg gatgttaaat atatattaat tcctgtgttc ctgattttgc
    37081 ccggtaatag agaattattc tctgagaagc tcatcatgat tgcaccatac aaatatcatg
    37141 atgataagag gatattattc attgatttaa acaaaatgac aaaatccata gacccatagt
    37201 ctgggtacta tataggagtg gtatgggcat caaacaacac aatgggaata ccaaagcaga
    37261 tcgtctcgct gaat
//
LOCUS       DAZPDU010000022        37260 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZPDU010000022 DAZPDU010000000
VERSION     DAZPDU010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37260
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.022"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..645)
                     /locus_tag="ACHURS_001001"
     CDS             complement(<1..645)
                     /locus_tag="ACHURS_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HFR1236793.1"
                     /translation="MVKKPQQGTIFAAAQRSDSYFVKCINMLSLYEKIKIRLIILFLL
                     AALSFIGLFFIINYQLVSERAVKRADSRFELIQKNVGYFFKDIERSALTLKDSLYLLK
                     NTEEIQRAVILKMEMMPFLDSVGLVLDDNKYYLFSRRANDKIVVYHQEQVNGPLVDES
                     GRVIFADFNPSKRPWSVASDDSNNSWNPAYNCFDRPGKKCISFTLRINGKDHDLL"
     gene            complement(745..1656)
                     /gene="gsiD"
                     /locus_tag="ACHURS_001002"
     CDS             complement(745..1656)
                     /gene="gsiD"
                     /locus_tag="ACHURS_001002"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFR1236794.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1659..2579)
                     /gene="gsiC"
                     /locus_tag="ACHURS_001003"
     CDS             complement(1659..2579)
                     /gene="gsiC"
                     /locus_tag="ACHURS_001003"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFR1236795.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGLEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2597..4135)
                     /gene="gsiB"
                     /locus_tag="ACHURS_001004"
     CDS             complement(2597..4135)
                     /gene="gsiB"
                     /locus_tag="ACHURS_001004"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFR1236796.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(4155..6026)
                     /gene="gsiA"
                     /locus_tag="ACHURS_001005"
     CDS             complement(4155..6026)
                     /gene="gsiA"
                     /locus_tag="ACHURS_001005"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFR1236797.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(6013..6978)
                     /gene="iaaA"
                     /locus_tag="ACHURS_001006"
     CDS             complement(6013..6978)
                     /gene="iaaA"
                     /locus_tag="ACHURS_001006"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFR1236798.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCIGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            7182..8417
                     /gene="moeA"
                     /locus_tag="ACHURS_001007"
     CDS             7182..8417
                     /gene="moeA"
                     /locus_tag="ACHURS_001007"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFR1236799.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            8417..9166
                     /gene="moeB"
                     /locus_tag="ACHURS_001008"
     CDS             8417..9166
                     /gene="moeB"
                     /locus_tag="ACHURS_001008"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFR1236800.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLIVGLGGLGC
                     AASLYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     TSGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(9345..10007)
                     /gene="fsa"
                     /locus_tag="ACHURS_001009"
     CDS             complement(9345..10007)
                     /gene="fsa"
                     /locus_tag="ACHURS_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFR1236801.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWQGAFGRT
                     SI"
     gene            10138..11037
                     /locus_tag="ACHURS_001010"
     CDS             10138..11037
                     /locus_tag="ACHURS_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFR1236802.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            11043..13475
                     /locus_tag="ACHURS_001011"
     CDS             11043..13475
                     /locus_tag="ACHURS_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFR1236803.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQTLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13621..14436
                     /gene="ybiV"
                     /locus_tag="ACHURS_001012"
     CDS             13621..14436
                     /gene="ybiV"
                     /locus_tag="ACHURS_001012"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFR1236804.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALNGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14588..15853
                     /locus_tag="ACHURS_001013"
     CDS             14588..15853
                     /locus_tag="ACHURS_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFR1236805.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSNMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGIDS"
     gene            complement(15820..16980)
                     /locus_tag="ACHURS_001014"
     CDS             complement(15820..16980)
                     /locus_tag="ACHURS_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR1236806.1"
                     /translation="MGFFAHENSVISELEADHKMDKKLKIADIAARTGLSPSTVSRVL
                     AGKANTSARAREQVLACARELGVMDGMAAGRMLLNNLIIFAPQRAFDQRSDIFYYRVI
                     QSISKALSPHEVRLRYCALEEFDSDPALFLSRMNEADTQAAILLGIDDPHIHDLAADL
                     AKPCVLINCRDERMRLPAIAPDHRLIGAFAARYLFEMGHREVMNVMCLRRYTMEQRFA
                     GIKESWRQHNLAFHHDRHLLNISSFSAKEAEEKVGAWLDAANGKTLPTAFLVGGDFMA
                     AGTVNALHRRGLRVPQDVSVMSIDGFNLAAIEDVPLTAVHVPRDELGNEAVQMLQQRL
                     IRPDAPVGSLLLHGRLAVRESVRRVRPGKGNTAVEREGLYDTQLSIPNARLP"
     gene            17218..18519
                     /locus_tag="ACHURS_001015"
     CDS             17218..18519
                     /locus_tag="ACHURS_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1236807.1"
                     /translation="MSQGINNDMTASKPRHIVKNLRWWILVLFLLGVTVNYITRNSLG
                     ILAPELKSSLGITTEQYSWIVGAFQLAYTLFQPLCGWLIDVIGLKLGFMICAGLWALA
                     CLMHAGAGNWVHLALLRFFMGGAEAAATPANAKTIGEWFPKSERPIAAGWAGVGFSIG
                     AMLAPPIIYFAHASFGWQGAFMFTGILAMLWVVLWWAFYHSPDKHPNLSKAELDFIRQ
                     DNEAPPVKLPFLTALKTVGKNKRFYGIAIPAFMAEPAWAVMSFWVPLYLSKTYGMDLK
                     QIALFAWLPFLAADLGSIASGYLTKLYTRLFGLSRVNSVVASSVSGAFLMVSLAVMAF
                     TNNPYIAIVLISIGGFGHQIISCMLSALVVESFDKGQMATVNGMRGSAAWIASFLFSL
                     IIGVTADKIGFNPLFIAMGFFDLIGAFFLVTFIAERRAKRA"
     gene            18534..20897
                     /locus_tag="ACHURS_001016"
     CDS             18534..20897
                     /locus_tag="ACHURS_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HFR1236808.1"
                     /translation="MKTLKNWTLHKQSAHHVELLVDGQHILCLYVLEENLFRVLLKRK
                     GELALDRTWSIAPAQDVPWEGRSREDLSGFSLPAWTVIQHDDTVVIATESLRATVHQP
                     LWLEWHYRNNEGEWLPLVNDRPTSAYLLNAHGDGVAHYQSRRKDERFYGLGDKAGDLQ
                     RNGKRYEMRNLDAMGYNAVSTDPLYKHIPFTITRRDDISFGLFYDNLSSCWLDLGNEI
                     DNYHTAYRRWQAEAGDIDYYLFTGERVLDITKAFVRLTGKTLFGPKWSLGYSGSTMHY
                     TDAPDAQNQLMNFIRLCEEHAIPCDSFQLSSGYTSINGKRYVFNWNYDKVPQPKVMSQ
                     AFHNAGLHLAANIKPCLLQDHPRYNEVAERGLFIRDSETDEPERSSFWDDEGSNLDFT
                     NPQTIQWWQEGVTTQLLEMGIDSTWNDNNEFEVWDGEARCHGFGQTIAIKHIRPVMPL
                     LMMRASMEAQQRFAPEKRPYLISRSGCAGMQRYVQTWSGDNRTSWDTLRYNTRMGLGM
                     SLSGLYNLGHDVGGFSGDKPDPELFVRWVQNGVMHPRFTIHSWNDDHTVNEPWMYPGV
                     TPAIRSAIELRYRLLPYFYTLMWQAYADDEPMLRPTFLDHEHDVQTFEECDDFLLGRD
                     ILVASVVEQGERQRRVWLPDNETGWYDFYNGEWFSGGQWITLDAPLEKLPLMVRAGAG
                     LPLSKRITYVSAEQDDTRELKLFPLKGVGTTSGLLFEDDGESWGYQTGNALWVEWEMV
                     CDGATINLKVNARGDYRPAWSALKVSLPLEEKRTLLVNGVEGSEWMR"
     gene            complement(21077..22672)
                     /locus_tag="ACHURS_001017"
     CDS             complement(21077..22672)
                     /locus_tag="ACHURS_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFR1236809.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERAIDFSGNYEDYLRSKGIDA"
     gene            22891..23811
                     /gene="ldtB"
                     /locus_tag="ACHURS_001018"
     CDS             22891..23811
                     /gene="ldtB"
                     /locus_tag="ACHURS_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR1236810.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(23870..24988)
                     /locus_tag="ACHURS_001019"
     CDS             complement(23870..24988)
                     /locus_tag="ACHURS_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFR1236811.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(24985..25452)
                     /gene="mntR"
                     /locus_tag="ACHURS_001020"
     CDS             complement(24985..25452)
                     /gene="mntR"
                     /locus_tag="ACHURS_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFR1236812.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            25638..25766
                     /gene="mntS"
                     /locus_tag="ACHURS_001021"
     CDS             25638..25766
                     /gene="mntS"
                     /locus_tag="ACHURS_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFR1236813.1"
                     /translation="MNEFKKCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            26038..27621
                     /gene="opgE"
                     /locus_tag="ACHURS_001022"
     CDS             26038..27621
                     /gene="opgE"
                     /locus_tag="ACHURS_001022"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFR1236814.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(27670..28185)
                     /gene="ompX"
                     /locus_tag="ACHURS_001023"
     CDS             complement(27670..28185)
                     /gene="ompX"
                     /locus_tag="ACHURS_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFR1236815.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(28238..28303)
                     /locus_tag="ACHURS_001024"
     CDS             complement(28238..28303)
                     /locus_tag="ACHURS_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1236816.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            28538..29425
                     /gene="rhtA"
                     /locus_tag="ACHURS_001025"
     CDS             28538..29425
                     /gene="rhtA"
                     /locus_tag="ACHURS_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFR1236817.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            29724..30227
                     /gene="dps"
                     /locus_tag="ACHURS_001026"
                     /gene_synonym="pexB"
     CDS             29724..30227
                     /gene="dps"
                     /locus_tag="ACHURS_001026"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFR1236818.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            30631..31377
                     /gene="glnH"
                     /locus_tag="ACHURS_001027"
     CDS             30631..31377
                     /gene="glnH"
                     /locus_tag="ACHURS_001027"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFR1236819.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            31516..32175
                     /gene="glnP"
                     /locus_tag="ACHURS_001028"
     CDS             31516..32175
                     /gene="glnP"
                     /locus_tag="ACHURS_001028"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFR1236820.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            32172..32894
                     /gene="glnQ"
                     /locus_tag="ACHURS_001029"
     CDS             32172..32894
                     /gene="glnQ"
                     /locus_tag="ACHURS_001029"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFR1236821.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            33011..35236
                     /gene="ybiO"
                     /locus_tag="ACHURS_001030"
     CDS             33011..35236
                     /gene="ybiO"
                     /locus_tag="ACHURS_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFR1236822.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPTPGATPAEPLPPGEPT
                     L"
     gene            complement(35233..36159)
                     /gene="rlmF"
                     /locus_tag="ACHURS_001031"
     CDS             complement(35233..36159)
                     /gene="rlmF"
                     /locus_tag="ACHURS_001031"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFR1236823.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            36435..36695
                     /gene="mcbA"
                     /locus_tag="ACHURS_001032"
     CDS             36435..36695
                     /gene="mcbA"
                     /locus_tag="ACHURS_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFR1236824.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            36960..>37260
                     /locus_tag="ACHURS_001033"
     CDS             36960..>37260
                     /locus_tag="ACHURS_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFR1236825.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNAPG"
BASE COUNT         9035 a        10058 c         9337 g         8830 t
ORIGIN      
        1 taacaaatcg tgatctttgc cgttgatacg tagcgtaaaa gagatacatt ttttacccgg
       61 acgatcaaag caattgtatg ccggattcca gctgttgtta gagtcatctg aagccaccga
      121 ccacggtcgt ttcgatgggt taaaatcggc aaaaataacc cgccctgact cgtcgacaag
      181 cggtccattt acttgttcct gatgataaac aacgatttta tcattcgccc tccgcgaaaa
      241 gagataatat ttattatcat caagtaccag tcccaccgag tctaaaaatg gcatcatttc
      301 cattttcaga atcacggcgc gttgaatctc ctctgtattt tttaataaat acagtgagtc
      361 ctttaatgtc agggccgaac gttcaatatc tttaaagaaa tagccaacgt ttttctgaat
      421 aagttcaaag cggctatcgg cacgttttac cgcgcgttcc gataccagtt gatagttaat
      481 gatgaaaaaa agaccaataa atgacagtgc tgccaataaa aataaaatta tcagccttat
      541 ctttatcttt tcgtataaac tcagcatgtt aatacattta acaaaataac tatctgaacg
      601 ctgtgccgct gcaaaaatag tcccttgctg cggcttttta accaaattac ccgattctac
      661 aaaatcagca gaaagttaca acacttgctt agggcaataa tttcctgtga ggtgattacc
      721 ctttcaagca atattcaaac gtaactatcc tttaattttc ggatccagcg catcgcgtaa
      781 accatcgccc aacaaattga acgccagtac ggtcagaaaa atagccaggg ccggaaaaac
      841 agcgacatgc ggcgcgataa ccatatccgc tcgtgcctca ttgagcattg ctccccactc
      901 tggtgtcggc ggctgcgcac cgaggccgag aaatgacagg ctggcggcag agataatcga
      961 ggtgccaatg cgcatggtga aaaacaccac gatagacgag acggtcccag gcaagatatg
     1021 acgcaataaa atggtcatat cgctggcacc aatactgcgt gctgactcaa taaaggtttg
     1081 ctgtttcaac accagcgtgt tgccgcgcac cagtcgggca aacgcgggga tggaaaaaat
     1141 ggcgactgcg ataatcacgt tagcaatgcc gcttcccaac accgcaacaa cagcgatcgc
     1201 cagtaaaata cccgggaagg caaacagcac atcgcaaatg cgcatgatca gccgatccca
     1261 ccagccttca taatatccag ccaacaagcc cagcaacgtc ccgatcgccg caccgataaa
     1321 cacggcaaac acgcccgccg ccagcgagat ttgcgcacca accaggacac ggctgaaaat
     1381 gtcgcgcccc agtgaatcga cgccaaacca gtgctgcaaa gaaggtccgt tattcagatt
     1441 gtcataatca aaataatttt cggcgtcata gggagcgatc cagcgtgcaa aaatggccac
     1501 cacaatcaat aaaataacga ataatgcggc ggtcatcgcc atatgctgac ggcgaaatcg
     1561 tcgccagaat tcatgccacg gtgtacgcac ctggtcaggt ttgaccagtg gcatggcgtt
     1621 taacaccgcc tgacgtcgcc agttaaatag tcgcatcctt acttgtacct gatagccggg
     1681 ttaatggcgg cgtaaagcac atccaccact aagttgataa gaataaattc cagcgagaaa
     1741 agcagaattt ccgcctgaat caccgggtaa tcgcgcattt ctacggagtc aacgagtaag
     1801 cgtccaagtc cgggccagtt gaaaactttc tccaccacga tagaaccacc gagcaaaaag
     1861 ccaaactgta agcccatcat ggtcactacc gggatcatcg cgttacgtag cccgtgtttg
     1921 aggacaaccc aggtttcgct cacccctttc gccctcgcgg tacgcatata atcttcgctt
     1981 aaaacatcga caaacgacgc gcgggtaaag cgcgccatca cggctgccac tgccgcgccg
     2041 agcgtcaggg agggtaaaat gtagtgctgc caactgtctg ctcccacggt aggcagccag
     2101 cccagttcaa cggagaatac ctgaattaaa agcatcccca gcgcaaatgc cggaaacgag
     2161 atccccgaca ccgcaatggt catactcaat cgatccggcc aacgattacg ccagacggcg
     2221 gcgataattc ccgccgccat accaaatatc accgcccaga ccatactggt tatggtcagc
     2281 cacagcgttg gcataaagcg gctggcaatc tcatcggcaa ccggacgacg cgacaccatc
     2341 gacaggccaa aatccccctg cacagcattg ctgatatagt gccagaactg gtgatacagc
     2401 ggctgatcca accccagctg ctgacgcacc agttctataa cctgcgcatc agcttcgagc
     2461 ccggcaatca atcgcgccgg atcgccgggc agcatatgca caaataaaaa caccagcacc
     2521 gagacgataa acagcgtcgg aatcaacccc agtaagcgtt tgataacgta attaagcatt
     2581 ccactccctg cgttgcttat tgcaaatccg cgtcttcaaa gctgaagccg gtgtctggca
     2641 tgatccaaaa accggtcagg tttttactgt gtgccgacac cagtttttct accaccagcg
     2701 ggatccacgg cgactcttgc cagatgatat cctgcgccgc cttatacaag cgggtctttt
     2761 ccgccggatc attagttttc agtgcctgag ccaggaagtc gtccacctgt ttattgctgt
     2821 aaaatgcggt attaaacagc gtcggtggcc agttttgcga ggcgaacagt ggcgatagcg
     2881 cccagtccgc ttcgccggtt gaagccgacc agccagtgta gaacatccgc acgccgctct
     2941 ctttttgccc tttaccttca acttctgccg cccgctgtcc ggcatccatc gcggtcacct
     3001 gggctttaat cccgacctgc gctaactgct gctgggtaaa ttgcagcact ttctgcgcgg
     3061 tgctgtggtt atgtgacgac cacagcgtgg tactgaaacc gttgggatat cccgcctctt
     3121 tcagtaattc gcgcgctttc actggatcgt aaggccacgg tttataactt tgcgcgtagg
     3181 cgatacttgg cggtaccaca ccagtagctg gcgttgcata gcccgcaaag gcaactttca
     3241 ccagcgccgg acggttaatg gcgtaattca gcgcctcacg gaccttcggg ttatcgaacg
     3301 gcttttgcgt cacgttcata ctgatataac gctgcataat tgacggactg gccatcaact
     3361 cgatattttt gtttttctcc agcagtgtgg cctgctcgta gggaatgggg aaagcaaact
     3421 gcgcttcacc ggtttgcagc attgccgcgc gggtgttgtt atccgccacc ggacgccagg
     3481 ttatgctgtc cagtttgggc aatcctggct gccagtaacc cgcgaatttt ttcaccttca
     3541 caaaatcggt ctgattccag gtatccagtt catacggtcc ggttcccacc ggatgaaaac
     3601 caatctcctt gccatatttt tccagcgctg ccggtgaaat catcgcggtc gccggatggg
     3661 caagaatatt aataaacgct gagaacggct gtttgagggt aatctttacc gttgtcggat
     3721 cgatcgcctc cgttttggca atattcttat acaggttata gcgtttaaga tgattcgccg
     3781 gatcgctggc ccggtccaga ttcgctttta ccgccactgc gttgaaatcg gtgccatcct
     3841 ggaatttaat gccttcccgc aatttcacgg tgtaagtaat gccgtcatcg gaaacggtat
     3901 aactctccgc cagcacgttt ttcagtttca tctctttatc cagaccgaac agcccctggt
     3961 aaaacgattt cgctacggcc tgagataacg tgtcattcgc gtcatacgga tcgagcgtgg
     4021 tgaaattcga tcctaccgcc accaccacat ctttggcagc gaatgcacaa gatgccatca
     4081 acgctgtcgc aatgcccagc gccactaacc cactacggtg tacagctctt gccattttat
     4141 tctccgcctg aatgttatct acgcatgaat gcgtattctg attgtggttg tgcgacgtaa
     4201 tgccccggcc cgacgcattg caacgagacg gctgccacct cttcgccacg cagatgaata
     4261 ttgctgggaa gatcgtccga cagcagtaca cgctgcggtc gttgtcggga cggttcagcg
     4321 accggaactg ccgccagtaa tttacgcgta taaggatgct gcgggttttc gaagaccgcg
     4381 cgccgtggac caatttcaac aatttgcccg agatacatca ccgccacacg atgactaatc
     4441 cgctctacca cggccatatc gtgggagata aacagatacg caatgccgaa atcacgctgg
     4501 agatcgagca acaagttgat aatctgcccg cgaatagaaa catccagcgc cgaaacggct
     4561 tcgtcggcaa tgatcacttt tggattcaat gccaacgcgc gagcaatgca gatgcgctgg
     4621 cgctgaccgc cggaaaactc atgcgggtaa cgccaggcat gttcaggtaa caggcccacg
     4681 cgctccagca accacgcaac gcgtgcagcc gcgtctttac ctggcaataa accgtgtaca
     4741 cgcagcggtt cgataatcga atcaccgatg gtctgacgtg ggtccagcga agcgtaaggg
     4801 tcctgaaaaa taaactgaat atcccggcgc aatgcctgaa gtttgccggg tgacaaggta
     4861 tcgattctct gaccgttaaa gataatttcg ccgccctgcg attcgaccag gcgcagcaac
     4921 gcccgcccgg tagtggattt accgctgcca gactcgccca ccagcgatag cgtttcgcca
     4981 ggccagagat caaaactgac tttctcaacg gcatgcactt cccgcgttac gcgattcaac
     5041 aaaccgctgc gcaaagggaa acgggtgaca agattacgca ctcgtaaaac aggttcgcca
     5101 tccaccaccg ttttctgctc gatggggggg gcctgtttcg ctggatgttc aagcgatatc
     5161 aacgggaaac gtcggggata atccaaccct ttcatcgcac caagttgcgg aacagcagct
     5221 aacagcgcac gggtgtaagg atgttgcggt gcatgaaaaa tctgttcgac ggtacccgtt
     5281 tccaccgcct cgccctgata catcaccagt acccgatcgg caatctctgc caccacgccc
     5341 atatcgtgag tgataaagat aacgcccatc gacatctctt tttgcaatac tttgattaat
     5401 tgcaggatct gcgcctgaat agtgacatcc agcgcggtgg ttggctcatc ggcaatcagc
     5461 accgccgggc ggcatgacag cgccatcgca atcatcactc gctggcgcat cccgccagag
     5521 agttgatgcg gataacgtga aagaatggtt tgtgcctcag gaatgcgtac ctgatccagc
     5581 atccgcttcg cctcgaccat cgcttcttca cgactggcgt tctgatgcag acgaattgat
     5641 tcggcaatct gttcacccac agtaaatacc gggttcagcg atgtcatcgg ctcctgaaat
     5701 atcatcgcca tatccgcacc gcgaacatgg cgcatttgtg cagcgctctg ctcgctaagt
     5761 tcaatcactt cgcgactgcg ccgctgcaac agcattttat cgcactgtac taaaccgccc
     5821 gcctgttcca acaggcgcat caatgccaac gcagtcactg acttaccgga gccggattcg
     5881 ccaacaattg ccagcgtctc accgcgttgc agactaaaag agagattgcg gaccgcagct
     5941 attttctgct ggtcctgcat aaaggcaata ttcagatttt caaccgccag cacattaccg
     6001 gcatcaagtt catcactgtg tggcaacggt gtcccctttt tcacggtaga taccggtggt
     6061 tggcgtatcg cctgcgtagc cccaggcgcg atacattcct tcggtgttaa acggtagcgc
     6121 gacattccct tcatggtcga tagcgattaa gccaccgcta ccgccaagcg cagggagttt
     6181 ttccattact acccgctcgc aggcttccgc gagacttaat ccgccgtaat ccattaacgc
     6241 ggcgatgtca tatgccgcca gcgcgcggat gaagacttcg cccgtgccga tacaagaaac
     6301 cgccacactg gcgttattgg cgtagcatcc ggcacccact aagggactat cgccaactcg
     6361 tccgggtaat ttattggtca ttccgcctgt ggacgtggct gccgccaaat tgccgtctaa
     6421 atccaacgcc acggccccca cggtgcccat tttctgtttt tcatccagtg gcgcaccgct
     6481 atggtcgagg acggttgccc cttccttgcg cgctgccagt agttgttcat aacgcaaagg
     6541 cgtggagaaa atctccggcg agacgcgctc catgccacga gcaaacgcaa aattttctgc
     6601 cccttcgcca atcatcatca catgcgggct ttgctccatc accagccggg cggcaagaac
     6661 cggattacgc agatgactaa cgcccgccac cgcaccggct ttcagggtgt taccatccat
     6721 cacacaggcg tccagttcat gggtttcatc acgcgtaaag acagcgccaa ttccggcgtt
     6781 aaacagtgga cactcttcca gcagacgcac cgcttccgtc accacatcca gcgcactttc
     6841 gcccgcttcc agcattttct gcccggtttc aacaatggca gacaacgcct caatgtagcg
     6901 taattcctgt tgcagactca tctgcgcgcg gctaattgca cctgcgccac catgaattgc
     6961 aatgactgct ttgcccatag tattcgtcca ttaagggtgg ctcgccactt ttgctataaa
     7021 tatcagaatt tttatgaatt actcattcaa tttttgaata tagaaagatg gatatggcat
     7081 gtaaaggcag gagccaacgc tacgcccgag gtggcataca gtctgtggcg tttttctgac
     7141 ataataggca aattcgattt tgcctccgca ggagtgtttt catggaattt accaccggat
     7201 tgatgtcgct cgacaccgcg cttaatgaga tgctttctcg cgtcacccca ctgaccgccc
     7261 aggaaacgct gccactggta cagtgttttg gtcgtattct ggcgagcgat gtcgtttcgc
     7321 cgcttgatgt tccggggttt gataactccg caatggacgg ctacgcggtg cgtttagccg
     7381 atattgcctc cgggcaaccg ctgcccgttg ccggtaaatc ctttgccggt cagccgtatc
     7441 atggtgaatg gcctgcgggc acctgcattc gtattatgac cggtgcgccg gtgccggaag
     7501 gctgcgaagc ggtggtgatg caggagcaga ctgaacaaac ggacaatggc gtgcgtttta
     7561 ctgctgaagt gcgtagcggg caaaatattc gccgtcgcgg tgaagatatc tctgcaggtg
     7621 cggttgtttt cccggcggga actcgcctga ctaccgcaga gctgccagta attgcttcac
     7681 tggggattgc cgaagttccg gtgattcgta aagtgcgtgt agcgcttttt tctaccggtg
     7741 atgaactcca gttgcccggt cagccgctgg gcgacggcca aatctacgac accaaccgtc
     7801 tcgccgtaca cctgatgtta gaacagctgg gttgcgaggt aatcaactta gggattatcc
     7861 gcgacgatcc ccatgccctg cgcgccgcat ttattgaagc cgacagccag gcggatgtag
     7921 tgatcagttc cggcggtgtt tcagtgggtg aagcggatta caccaaaacg attcttgaag
     7981 agctggggga gatcgccttc tggaagctgg cgattaaacc aggtaaaccg ttcgcgttcg
     8041 gtaaactcag caatagctgg ttctgcggcc tgccgggcaa cccggtttca gcgacgctga
     8101 ccttctatca actggtacag cctttgctgg caaaactaag cggcaacacc gccagcggcc
     8161 tgcccgcgcg ccagcgcgta cgcacagcgt cccgcctgaa aaaaacgcca ggacgtcttg
     8221 atttccagcg cggcgtgctg caacgcaacg ccgacggtga acttgaagtg acgaccaccg
     8281 gacatcaggg ttcacatata tttagctcct ttagtctcgg caactgcttt atcgtgctgg
     8341 aacgcgatcg cggcaatgtg gacgtgggcg aatgggtgga agtagaaccg tttaacgcgt
     8401 tgttcggagg cctgtaatgg cggaactcag cgatcaggag atgctgcgct acaaccggca
     8461 aatcattctg cgcggttttg attttgacgg tcaggaggcg ctaaaagatt ctcgtgtgct
     8521 gatcgtcggc ctcggtggac tcggctgcgc agcttcgctg tatctggcaa gcgccggtgt
     8581 cggcaacctg acgctgctcg acttcgacac ggtttcgctc tcgaatctgc aacgccagac
     8641 actgcacagt gatgccacgg tcgggcaacc gaaggtggaa tccgcccgtg acgccctgac
     8701 gcggatcaac ccacatatcg cgattacgcc agtcaatgca ctgctagatg acgcagaact
     8761 tgcagcattg attgctgaac acgatctggt gctcgactgt acggataacg ttgcggtacg
     8821 taatcaactg aacgcaggct gttttgccgc gaaggtgccg ctggtttccg gcgcggcaat
     8881 tcgtatggaa gggcaaatca cggtcttcac ttatcaggac ggcgaaccgt gctatcgctg
     8941 ccttagccgt ttgtttggcg agaacgcatt aacctgcgta gaagcaggcg tgatggcacc
     9001 gttgatcggc gtaattggtt cgttgcaggc aatggaagcg atcaaactgc tggcaggtta
     9061 tggcaaaccg accagcggga aaatcgtcat gtacgatgcg atgacctgtc agtttcgcga
     9121 aatgaaactg atgcgcaatc cggggtgtga ggtgtgcggg cagtaattgc cacctccagg
     9181 cctgataaga cgcgacaagc gtcgcatcag gcatttgctc cagacgccag atgcggcgtg
     9241 aacgccttat ctggcctacg gttcgggcac agatttccaa gcgcggcagg catcggtgca
     9301 ctccgccacg tcatcagcaa tttatgagga tgcagaacgt ccggttaaat cgacgttctg
     9361 ccaaacgctc cctgccagtc ctgctcaaac ttcgccacag cggcttccac cgccggatag
     9421 ctaatcatct gttgtgccac atccagtggc agagtaattg attcacatcc tgccagtaag
     9481 cagtccagcg cctgacgcgg ggttttgaaa ctcgctgcca gcactttcgc ctgcggcgca
     9541 tgcattttca ataactggtg taagtcggtc acagtctgaa tgccgctacc gccctgagca
     9601 tcaatacgat taacgtaagg cgcaacatat tccgcacctg ccagcgccga cagcaaccct
     9661 tgtgctgcgc catataccgc ggttcccagc gttggaatcc cttccgcttt taacatctta
     9721 atagctgcca gcccctcggc ggtcaccgga actttcacca cgatatccgc aataatagaa
     9781 cgcaatttac gtgcgtcatt caccatccct tcagcagtgg tagccattac ctgggcaaac
     9841 agacgcccct gaccgcccat cgcttcatga agttgcggaa gcacaacatc cagcggtttt
     9901 ttacccgcgg cgataatgct tgggttagtg gtcacacccg ccagcggaaa aatacgtgac
     9961 agcgccttca ccgcaacaac gtctgaagta tccagataca gttccataac catcctcaaa
    10021 atgtactttt tgtctgaatg tagactatca cgggaaaact ctctgaacag ttgacgcaca
    10081 tcaatctaac tttcattcga aagtatttta atgtttgaac gaaacagaga ggcaactatg
    10141 attttcaata ttcagcgcta ctcgacccat gatggccccg gtatccgcac ggtcgtattt
    10201 cttaaaggct gttcgctggg ctgccgctgg tgtcagaacc cggaaagccg cgcccgcacg
    10261 caggatctgc tgtatgacgc acgactgtgt ctggaaggct gcgagctgtg cgctaaggcc
    10321 gcgccggaag tgattgagcg cgcgctgaat ggtttgctta ttcatcggga aaagttaacc
    10381 ccggagcatc tgacggcgtt aaccgactgc tgtccgacac aggcattaac cgtgtgtggt
    10441 gaagtgaaaa gcgttgagga gatcatgacg accgttctgc gcgataaacc gttttacgat
    10501 cgcagcggcg gcggtttaac gctttcgggt ggtgagccct ttatgcagcc ggaaatggcg
    10561 atggcgctac tgcaagccag ccacgaggca ggcattcata ctgcggtaga aacctgtctg
    10621 catgtgccgt ggaaatatat cgccccttct ctgccctata tcgatctgtt tcttgccgat
    10681 ttaaaacacg ttgccgacgc gccgtttaaa cagtggaccg acggtaacgc cgccagagtg
    10741 ctggataacc tgaaaaaact cgccgcagcg ggcaaaaaaa tcattatccg cgtgccgctg
    10801 attcagggct ttaatgccga cgaaacctct gtaaaagcta ttaccgattt tgccgccgac
    10861 gagctgcacg ttggcgaaat tcatttcctg ccctaccaca cgctaggcat caacaaatat
    10921 cacttactta atctgcccta tgacgccccg gaaaaaccgc ttgatgcgcc agaactgctc
    10981 gactttgccc agcagtatgc ctgccagaaa gggttaaccg cgaccttacg aggataacaa
    11041 ccatgaccac actgaaactg gacacgctca gcgaccgcat taaagcgcac aaaaatgcgc
    11101 tggtgcatat tgtgaaaccg ccagtctgta ccgagcgcgc gcagcactat accgagatgt
    11161 atcaacaaca tctcgataag ccgatcccgg tacgtcgcgc gctggcactg gcgcatcacc
    11221 tggcgaatcg caccatctgg atcaaacacg atgagttgat cattggcaac caggcaagcg
    11281 aagttcgcgc cgcgccgatc ttcccggaat atactgtctc gtggatcgaa aaagagattg
    11341 atgatctggc agatcgtccc ggtgctggct ttgcggtgag cgaagagaac aaacgcgttc
    11401 tgcatgaagt gtgcccgtgg tggcgcggtc agaccgtaca ggatcgctgc tacggcatgt
    11461 ttaccgatga gcaaaaaggt ctgctggcga ccggaatcat taaagcggaa ggcaatatga
    11521 cctccggcga tgcgcacctg gcggtgaatt tcccgctgct gctggaaaaa ggtcttgatg
    11581 gtctgcgcga gaaagtagcg gaacgtcgct cgcgcatcaa tctgacggta ctggaagatt
    11641 tgcacggcga acagttcctg aaagcgattg atatcgtgct ggtggcagtc agcgaacaca
    11701 ttgaacgttt tgcagccctg gcgcgtgaaa tggccgcgac cgaaacccgc gaaagccgtc
    11761 gcgatgaact gctggcgata gcagaaaact gcgatcttat cgcccaccag ccgccgcaga
    11821 ctttctggca ggcgctgcaa ctgtgttact tcatccaatt gatattgcag atcgaatcta
    11881 acggtcactc ggtatcgttt ggtcgtatgg accagtatct ctacccgtac tatcgccgcg
    11941 acgttgaact caaccagaca ctggatcgcg aacacgccat cgaattgctg catagctgct
    12001 ggctgaaact gctggaagtg aacaagatcc gctccggctc acactcaaaa gcctctgcgg
    12061 gaagtcctct gtatcagaac gtcaccattg gcggacaaac tctggttgat ggtcaaccaa
    12121 tggacgcggt gaatccactc tcttacgcta tcctcgaatc ctgcggtcgc ctgcgttcca
    12181 ctcagcctaa cctcagcgtg cgttaccatg caggaatgag taatgatttc ctcgacgcct
    12241 gcgtacaggt gatccgttgc ggcttcggga tgccggcgtt caacaacgac gaaatcgtga
    12301 tcccggaatt tattaaactc ggtattgaac cgcaggacgc ttacgactac gcagcgattg
    12361 gttgtatcga aaccgccgtc ggtggcaaat ggggctatcg ctgtaccggc atgagcttta
    12421 tcaacttcgc ccgcgtgatg ctggcggcgc tggaaggcgg gcgtgatgcc accagcggca
    12481 aagtgttcct gccacaagaa aaagcgttgt cggcaggcaa ctttaacaat ttcgatgaag
    12541 taatggacgc gtgggatacg caaatccgtt attacacgcg taaatcaatc gaaatcgaat
    12601 atgtcgtcga caccatgctg gaagagaacg tgcacgatat tctctgctcg gcgctggtgg
    12661 atgactgtat tgagcgagcg aaaagtatca agcaaggcgg cgcgaaatat gactgggttt
    12721 ctggcctgca ggttggcatt gccaacctcg gcaacagcct ggcggcagtg aagaaactgg
    12781 tatttgaaca gggcgcgatt ggtcagcaac agctggctgc cgcactggcg gatgacttcg
    12841 atggcctgac tcatgagcaa ctgcgtcagc ggctgattaa cggtgcgccg aagtacggca
    12901 acgacgatga taccgtcgat acgctgctgg ctcgcgctta tcagacctat atcgacgaac
    12961 tgaaacagta ccataacccg cgctacggtc gtggtccggt tggcggcaac tattacgcgg
    13021 gtacctcgtc tatctccgct aacgtaccgt ttggcgcgca aactatggca acaccggacg
    13081 ggcgtaaagc ccacaccccg ctggcagaag gcgcaagccc ggcctccggt actgaccatc
    13141 ttggccctac tgcggtcatt ggctcagtgg gtaaactgcc tacggcagcg attctcggcg
    13201 gcgtgttgct caaccagaaa ctgaatccgg caacgctgga gaacgaatct gacaagcaga
    13261 aactgatgat cctgctgcgt accttctttg aagtgcataa aggctggcat attcagtaca
    13321 acatcgtttc ccgcgaaacg ctgctggagg cgaaaaaaca tccggatcag taccgcgatc
    13381 tggtagtgcg tgtcgcgggc tattccgcct tcttcaccgc gctctctcca gacgcacagg
    13441 acgatatcat cgcccgtact gaacatatgc tgtaatctcc tcggcccggc ggcgtcgtcg
    13501 ccgggctaaa tcattcacat catcaatttc atccttactt tcattcgaaa tataatttgt
    13561 gctctgcgtc acattgttat tacactgtta cggtttcatt ttcgagccag gagcacacct
    13621 atgagcgtaa aagttatcgt cacagacatg gacggtactt ttcttaacga cgccaaaacg
    13681 tacaaccaac cacgttttat ggcgcaatat caggaactga aaaagcgcgg cattaagttc
    13741 gttgtcgcca gcggtaatca gtattaccag cttatttcgt tctttcctga gctaaaggat
    13801 gagatctctt ttgtcgcgga aaacggcgca ctggtttacg aacatggcaa gcaactgttc
    13861 cacggcgaac tgacccgaca tgaatcgcgg attgttattg gcgagttgct aaaagataag
    13921 caactcaatt ttgtcgcctg cggtctgcaa agtgcatatg tcagcgaaaa tgcccccgaa
    13981 gcatttgtcg cactgatggc aaaacactac catcgcctga aagctgtaaa agattatcag
    14041 gagattgacg acgtactgtt caagttttcg ctcaacctgc cagatgaaca aatcccgtta
    14101 gtgatcgaca aactgcacat agcgctcaat ggcattatga aacccgtcac cagtggtttt
    14161 ggctttatcg acctgattat tcccggtcta cataaagcaa acggtatttc gcggttactg
    14221 aaacgctggg atctgtcacc gcaaaatgtg gtagcgattg gcgacagcgg taacgatgcg
    14281 gagatgctga aaatggcgcg ttattccttt gcgatgggca atgctgcgga aaacattaaa
    14341 caaatcgccc gttacgctac cgatgataat aatcatgaag gcgcgctgaa tgtgattcag
    14401 gcggtgctgg ataacacatc cccttttaac agctgacctc tctaccggag ccgtgcgctc
    14461 cggttttccc ttctttttac atcctgtaca tcacatcaaa tcattaaact tgcattaact
    14521 tatttataac ttatattgtc gcatgcaaat cagaatggtt tcgaatgaaa gttaatgaga
    14581 ggtggttatg gcttctactt ttaccagcga cacattgcct gccgatcaca aagcagctat
    14641 ccgtcagatg aagcacgcgc tgcgggcgca gcttggcgac gtccagcaga tctttaatca
    14701 gctaagcgat gacattgcca cgcgagtggc tgaaatcaac gcactcaaag cacagggcga
    14761 tgccgtctgg ccggtgctgt cttatgccga tatcaaagca ggtcatgtta ctgcagagca
    14821 gcgcgaacag attaaacgtc gcggttgtgc ggtgataaaa ggccatttcc cccgcgaaca
    14881 agcgctaggc tgggatcagt cgatgctgga ctatctggac cgcaaccgct ttgacgaggt
    14941 ctacaaaggc cccggcgata atttcttcgg gacgctcagc gcttcacgtc ccgagattta
    15001 ccccatctac tggtcgcagg cgcaaatgca ggcccgccag agtgaagaaa tggcgaatgc
    15061 gcagtcgttt ctcaatcgtc tgtggacatt tgaaagtgat ggaaagcaat ggtttaaccc
    15121 ggatgtgagc gtcatctacc ctgaccgtat ccgccgccgt ccgcccggaa cgacctccaa
    15181 aggtcttgga gcgcataccg actccggggc gctggaacgc tggctgcttc cagcgtatca
    15241 gcgcgttttc gccaacgtct ttaatggcaa tctggcgcaa tatgatccct ggcatgcggc
    15301 acatcgtacg gaagttgaag agtacacggt ggacaacacc accaaatgtt ccgtgtttcg
    15361 gacattccaa ggctggacag cgctctctaa tatgctgcct ggtcaggggc tgctgcacgt
    15421 tgtgcccatt cctgaagcca tggcgtacgt actgttacgt ccgctgcttg atgatgtgcc
    15481 ggaggatgaa ctgtgcggcg tagcgcccgg aagagtgttg ccggtatcag agcaatggca
    15541 tccactgtta attgaggcgt taaccagcat tccaaaactc gaggccggag actccgtctg
    15601 gtggcactgc gacgtcatcc attccgttgc ccccgttgaa aatcaacagg gttggggcaa
    15661 cgtgatgtac attcctgcgg caccaatgtg cgagaaaaat cttgcctacg cgcacaaggt
    15721 gaaggccgca ctggaaaaag gcgcatcgcc gggcgacttc ccgcgcgagg actatgaaac
    15781 aaactgggaa ggacgcttta cgcttgccga cctcaacatt cacggtaagc gagcgttggg
    15841 aatcgacagc tgagtgtcgt acagcccttc ccgttccacg gcggtgttcc cttttcctgg
    15901 gcgaacccgc cgcactgact cccgcaccgc caatcgtccg tgcagcagca gcgagcccac
    15961 cggagcatcc gggcgaatca gccgctgttg caacatttgc accgcttcat tgcctagctc
    16021 atcgcgtggc acatgcacag ccgtcagggg aacatcctcg attgccgcaa ggttaaagcc
    16081 gtcgatactc atcaccgaga catcctgtgg tacgcgcaga ccacgacgat gtagcgcatt
    16141 taccgttccc gccgccataa aatccccgcc gaccagaaac gccgtcggca acgtcttgcc
    16201 attggcggca tccagccacg ctccgacctt ttcttccgct tctttagcgc taaaactgct
    16261 gatattcagc aaatgacggt catggtgaaa ggccagatta tgctgacgcc atgattcttt
    16321 aatccctgca aaccgctgct ccatagtgta gcggcgcagg cacatcacat tcatcacctc
    16381 ccggtgaccc atttcaaaca gatagcgggc ggcgaacgcg ccaattaacc gatgatccgg
    16441 cgcaatcgcc gggaggcgca ttcgttcatc acggcagttg atcagcacac aaggcttagc
    16501 gagatcggcg gccagatcat ggatgtgcgg atcgtcaatc cccagcagga tcgctgcctg
    16561 cgtatctgct tcattcatcc gcgagagaaa gagcgcggga tcgctgtcaa actcctccag
    16621 cgcgcagtaa cgcagccgca cttcatgcgg cgataacgct ttgctgatgc tctggatcac
    16681 acggtaataa aagatatcag atcgttggtc aaaagcgcgt tgcggcgcga aaataatcag
    16741 gttgttcagc aacatacgac ctgctgccat gccatccatc acgcccaact cacgcgcgca
    16801 ggccagcacc tgttcgcgag cgcgtgcact ggtattcgct ttgccagcca gcacccgcga
    16861 aaccgtactc ggcgatagcc cggtacgtgc ggcgatatcg gcgattttca gcttcttgtc
    16921 cattttgtga tctgcctcta attcggaaat gacagaattt tcatgagcaa aaaagcccat
    16981 tctgtctggc attaaatcca tttcagtaat gtatatcagt cactcatgga aaaacgtgca
    17041 caaaacccgc tttctttcgc ttttagggtc tcttactgtg aattggtaga acaacttcca
    17101 tacaaaaata atggaaaaac gaacaataaa gactttattt atcaaaaagt taaaagtggt
    17161 gtaacaactc cattctgacg ttgccgcaca aaccctatac cattctgtgg agtgcatatg
    17221 agtcagggta tcaataacga tatgactgcc agtaaaccgc gccatatcgt caaaaatctg
    17281 cgttggtgga tactggtcct ttttttgctc ggtgttactg tcaactacat cacgcgtaac
    17341 tcgctgggta ttctcgcccc cgaactgaaa agcagcctag ggattaccac cgaacaatat
    17401 tcctggattg taggtgcttt ccagctcgcc tataccctat tccaaccgtt gtgcggatgg
    17461 ctgattgacg tcattggcct caaactgggc tttatgatct gcgcgggact gtgggcactg
    17521 gcctgtctga tgcatgcggg ggcgggaaac tgggtacatc tggcgctgtt gcgcttcttt
    17581 atgggcggtg cggaagccgc ggcaacccca gcaaacgcca aaaccatcgg tgaatggttt
    17641 ccaaaatcag aacgcccgat cgccgcaggc tgggcaggcg tcggtttttc catcggtgcc
    17701 atgctggccc caccgattat ctattttgcc cacgcttcat tcggctggca gggtgcattt
    17761 atgttcactg gcatcctggc gatgctgtgg gtggtgctgt ggtgggcgtt ctatcacagc
    17821 ccggataagc atcctaacct cagcaaagcc gagctggact ttattcgcca ggataacgaa
    17881 gcgcccccgg tcaagttacc ctttctgacc gcactgaaaa ccgtgggcaa aaataaacgc
    17941 ttttacggta tcgctatccc ggcctttatg gcagaaccgg cgtgggcggt gatgagtttc
    18001 tgggtgcctc tatacctgtc caaaacctat ggcatggacc tgaagcagat cgcattattc
    18061 gcctggttgc cgttcctcgc cgctgatctt ggcagtattg ccagcggcta tctgaccaaa
    18121 ctttacaccc gcctgtttgg gctgagtcgt gtgaactccg ttgtcgcatc atcggtcagt
    18181 ggcgcattct tgatggtttc gctggcggtg atggccttta ccaataaccc gtatatcgcc
    18241 atcgtcctta tctccatcgg cggcttcggt caccagatca tctcctgcat gctgagcgca
    18301 ctggtggtgg aatccttcga caaaggtcag atggccactg ttaacggcat gcgcggttct
    18361 gccgcgtgga tcgcaagttt cctgttctcc ctgattatcg gggttaccgc tgacaaaatc
    18421 ggctttaacc ccctgtttat cgccatgggc ttctttgacc tgattggcgc tttcttcctg
    18481 gtcaccttta ttgctgaacg tcgcgcaaaa cgcgcctgag agtggatgta aacatgaaaa
    18541 cgctgaaaaa ctggaccctg cacaaacagt ctgcccacca cgttgaactg ctggtcgatg
    18601 gccagcatat cctgtgcctg tacgtgctgg aagaaaatct gttccgcgtg ctgttaaaac
    18661 gcaaaggcga gctggcgcta gatcgcacct ggagcattgc tccggcgcag gatgtgccat
    18721 gggaagggcg cagccgcgaa gatctcagcg gctttagcct gcccgcgtgg acagtgatcc
    18781 agcacgacga caccgtggtt atcgccaccg agtcactgcg tgcaaccgtt caccagccgc
    18841 tgtggctgga gtggcactat cgtaacaacg aaggcgaatg gctgccgttg gtgaatgacc
    18901 gcccgaccag cgcttatctg ctgaatgccc acggcgatgg cgtggcacac tatcagagcc
    18961 gtcgtaaaga tgaacgcttt tatggcctcg gagataaagc cggcgatctg caacgcaacg
    19021 gtaaacgtta cgagatgcgt aacctcgacg cgatgggcta caacgccgtt agtactgacc
    19081 cgctgtacaa acacattcca ttcaccatca cccgccgcga tgacattagc ttcggcctgt
    19141 tttatgacaa cctcagcagc tgctggctgg atctgggcaa cgagatcgac aactaccaca
    19201 ccgcctatcg ccgctggcag gcggaagcgg gtgatatcga ctactacctg tttaccggcg
    19261 aacgcgtact tgatatcacc aaagccttcg tacgcctgac cggaaaaacc ctgttcggac
    19321 cgaaatggag cctgggctac agcggttcaa ctatgcacta caccgatgcg ccggacgcac
    19381 aaaatcagtt gatgaacttt atccgcctgt gtgaagaaca tgcaattccc tgcgattcgt
    19441 tccagctctc ttccggttat acctcaatta acggcaaacg ctatgtgttc aactggaact
    19501 acgacaaagt gccgcagccg aaagtgatga gccaggcatt tcacaatgct ggtttgcacc
    19561 tggccgccaa tatcaagccg tgcctgttgc aggatcaccc acgctataac gaagtggcag
    19621 aaagaggact gttcatccgt gattcagaaa cagatgaacc agaacgttcc agcttctggg
    19681 atgacgaagg ttctaacctc gactttacta atccgcagac catccagtgg tggcaggaag
    19741 gcgtgaccac acagcttctg gagatgggta tcgactccac ctggaacgac aacaacgaat
    19801 tcgaagtatg ggacggtgaa gcacgctgtc acggctttgg tcagacgatc gcgatcaaac
    19861 atattcgccc agtgatgcca ctgctgatga tgcgagcctc gatggaagcc cagcagcgtt
    19921 tcgctccgga aaaacgtccg tacttgatct cccgctccgg ctgcgcggga atgcagcgct
    19981 acgtgcagac ctggagtggc gataaccgca ctagctggga cactctgcgc tacaacaccc
    20041 gcatgggtct ggggatgagc ctctccgggc tgtataacct cggtcacgac gtcggcggtt
    20101 tctccggtga taagccggac ccggaactgt ttgtacgctg ggtacaaaac ggcgtgatgc
    20161 atccgcgctt tacgattcac tcatggaacg atgaccatac cgtaaacgaa ccgtggatgt
    20221 atccgggcgt cactccggcg atccgcagcg ctattgagtt gcgctatcgc ctgctgccct
    20281 acttttatac cctgatgtgg caggcatacg ccgatgatga accaatgcta cgtccgactt
    20341 tccttgacca cgagcacgac gttcagactt ttgaagagtg tgatgacttc ctgcttggtc
    20401 gcgatatttt ggttgccagc gtcgtagagc aaggcgagcg ccagcgccgt gtatggctgc
    20461 cagataacga aactggctgg tacgatttct acaacggcga atggttcagt ggcggtcagt
    20521 ggatcaccct cgacgcaccg ctggagaaac tgccgctgat ggttcgtgcc ggggctggtc
    20581 taccgctcag caaacgcatc acctatgtga gcgcagaaca agacgatacc cgcgaactga
    20641 aactgttccc gctgaaaggt gtgggcacaa cgtccggcct gctgtttgaa gacgatggcg
    20701 aaagctgggg ctaccaaact ggtaatgcgc tttgggtgga atgggaaatg gtttgcgatg
    20761 gggcgaccat caacctgaaa gtcaacgcgc gcggggatta tcgtcctgca tggagtgcac
    20821 tgaaagtgtc attaccattg gaagaaaaac gcacgctgct ggtcaatggt gttgaaggga
    20881 gtgaatggat gcgttaaatc attaccgggg ccgggaggcg actgcacctt acccggtcca
    20941 gccatcgaat cctttacctc agtatttcac gctgcaggaa ggcagcaaac ctgtaaaccc
    21001 cgggagctta aataagtaag tgaccggggt gaacaggtga agccaatgca tctgccgcat
    21061 gaaagacggc ggggaattaa gcgtcgatcc ctttactacg caggtaatct tcgtaattac
    21121 cgctaaagtc gatcgcgcgt tccggggtga tttccagaat gcgggtcgcc agggagctta
    21181 cgaactcacg gtcgtgtgaa acaaagatca gcgtgccctg atacagttcc agcgccatgt
    21241 tcagtgactc gatggattcc atatccaggt ggttggtcgg ttcgtccatg atcagaatgt
    21301 tcggcttctg catcattaac ttaccaaaca gcatccgccc tttttcccca ccggaaagca
    21361 ctttagctgg ctttttgatg tcgtcctggc tgaacagcaa acgaccgaga atgctgcgta
    21421 ccgcctgctc gtcatcgcct tcctgcttcc actgactcat ccattcgaac acggtcagat
    21481 cattttcaaa ctcatattcg tggtcctggg cgtagtaacc aatgcgcgcg ttttcagacc
    21541 attttacggt gccgctgtcc ggttgcagat cgcccaccag cgttttcagc agcgttgatt
    21601 taccgacgcc gttggtaccc agtaccgcaa gtttttcacc gacctccagc agcaggttga
    21661 gatttttaaa cagtggaccg ttatcaaacc ctttggtcag accttccact tccagcgcgt
    21721 tacggaacag tttcttatcc tgttcaaaac ggatgaacgg gttctgacgg ctggaggctt
    21781 tcacctcttc cagtttgatt ttatcaatct ggcgcgcgcg cgaagttgcc tggcgagatt
    21841 tcgaggcgtt ggcgctaaag cggctaacga aagattgcaa ctcagcaatc tgcgctttct
    21901 tcttggcgtt atcggccagc agacgttcac gcgcctgggt cgccgccgtc atgtactcat
    21961 cgtagttacc cggataaacg cgcagctcgc cgtaatccag atccgccatg tgggtacaga
    22021 ccatgttaag gaagtgacgg tcgtgcgaga tgatgatcat ggtgctgtca cgctcgttca
    22081 gcacctgttc cagccagcga atggtgtcga tgtcgaggtt gttggtcggt tcgtcgagca
    22141 ggagaatatc cggatcagca aacagcgcct gcgccagaag cacacgcagt ttccagccag
    22201 gagcaacttc actcatcggg ccgtagtgct gttccactgg aattcccacg ccaagcaaca
    22261 gttcaccggc gcgagcttcc gcagagtaac cgtccatttc gccgtattta acttccagat
    22321 cggccacttt atagccgtct tcctcactca tttccggcaa agcatagatg cggtcacgct
    22381 cctgcttcac ttcccacaac tctttatgcc ccatgatcac cgtatccagc acagtgaact
    22441 cttcaaaggc aaactgatcc tgacgcagct taccaatgcg ctcgttggga tcgagggaaa
    22501 cgttacccaa agtcggctct aagtcgccgc cgaggatctt cataaaggtg gatttaccac
    22561 taccgttcgc gccaatcagg ccgtaacggt tgccgccgcc gaatttgacg gaaatgtttt
    22621 caaacaacgg cttactgccg aactgcatgg tgacgttact ggaaactaac acaggcgtat
    22681 cctgaaaaga gatatgacaa accgcgcatt atgccataag tctgaaagaa gatcctgctc
    22741 ctcgcttaag gtctacactc aagccaaagg caaaaaatgt gatttcgtac acatctgatt
    22801 tcactgtgag ctggaatgaa cttataatgc gcttccaata ctctaatatt ctcaacccaa
    22861 tggcctgcca ggcacaaaat ctcgcttaac atgaatatga aattgaaaac attattcgca
    22921 gcggccttcg ctgttgtcgg cttttgcagt accgcctctg cggtaactta tcctctgcca
    22981 accgacggga gtcgcctggt tggtcagaat caggtgatca ccattcctga aggtaacact
    23041 cagccgctgg agtattttgc cgcggagtac cagatggggc tttccaatat gatggaagcg
    23101 aacccgggtg tggatacctt cctgccgaaa ggcggtactg tactgaacat tccgcagcag
    23161 ctgatcctgc cggataccgt tcatgaaggc atcgtcatta acagtgcaga gatgcgtctg
    23221 tattactatc cgaaagggac caacaccgtt atcgtgctgc cgatcggcat tggtcagtta
    23281 ggcaaagata cgcctatcaa ctggaccacc aaagttgagc gtaagaaagc aggcccgacc
    23341 tggacgccga ccgccaaaat gcacgcagag taccgcgctg cgggcgaacc gcttccggct
    23401 gtcgttccgg caggtccgga taacccgatg gggctgtatg cactctacat cggtcgcctg
    23461 tacgctatcc atggcaccaa cgccaacttc ggtatcggcc tgcgtgtaag tcatggttgt
    23521 gtgcgtctgc gtaacgaaga catcaaattc ctgttcgaga aagtaccggt cggtacccgc
    23581 gtacagttta ttgatgagcc ggtaaaagcg accaccgagc cagacggcag ccgttatatt
    23641 gaagtccata acccgctgtc taccaccgaa gcccagtttg aaggtcagga aattgtgcca
    23701 attaccctga cgaagagcgt gcaaacagtg accggtcagc cagatgttga ccaggttgtt
    23761 cttgatgaag cgattaaaaa ccgctccggg atgccggttc gtctgaatta atcttcaaaa
    23821 cttaaagcaa aaggcggact cataatccgc cttttttatt tgccagaccc tagttggccg
    23881 ggagtataac taacaaaaca tatcccacca acgccgccca caacagcatc ggtattgaat
    23941 agagatggaa acgccaccag atgcggcgat cgttcgccat acgtagcgca atcaaatttg
    24001 ccagcgatcc gggtaataac ccaaagccac ctacgtttac cgcccatacc agtaataaag
    24061 acggcggcac atagttcagc aacaatatgg tactcggcac gttactgatc acctgcgata
    24121 aaccgattgc cgttaaccat aacccgggtt cagatagatg actcacgtta cccaacacgc
    24181 cttgcaacgc tggaagctgg gtcagtaaat ggacgtcgat aaacatcgcc ataaacacca
    24241 gcagcagcgt ccagtccaca ctgagcacca cgcgacgtgc cagcagcgca aagcctgccg
    24301 ccacaatcac cagtccccac agctcttgtt tgaactccag cgccgtcaga aagacgatat
    24361 acagccccag acaactccac accagccgcg gtttccactc cggtgtttgc acccccgtat
    24421 ggtattgcag cgcctttcca gggaaacaac accagcacag gagcaggagc gtcagcatca
    24481 ttgcgccagc cagcggtgcc atttgggcaa taaatccggc aaacgaaaga ccagaacgtc
    24541 cccagataag aatattttgc gggttgccaa ttggcgtcag tagcgaacca gcgttgactg
    24601 ccagcgcctc aaaaataatc agccgattaa ccgggatctc acacagtctt tttagcgtga
    24661 tagtcagcgg aacaacaata aacagcgcga catcgttggt cagaaaggta gaaagcagcg
    24721 ccgccgccag caccataaac atcgccagcc gacgctccgt agcaaagcgg cgcaccattt
    24781 tgcgccccag cacatcaaaa taaccgctta actccacacc tttggtcagc agcatcaggc
    24841 cgcttaaggt gatgatggtg tgccagtcga tagcagcagg ccaggatttc ggtgcaaagg
    24901 gcacgaaaaa gcttaatccg ataccaacaa gaattaataa ctgaaaaaaa cgatcgcctt
    24961 gcagcgtgcg taaaaaaggc aggctcattt ggcaccgtgt ttctgggtaa acaaacgaaa
    25021 agcgtcgagc gtctcttcac taacatggtg ctccatgcct tccgcgtcgc gacgggcgat
    25081 ttccggactg acgcccaaca ccaacaagaa attttcgact atctgatgtc gctcgcggct
    25141 ttcctgcgcc agcttctctc cttctgccgt taaaaacacg ccacgccagg ggatcatttc
    25201 aatcagcccc atggttgcca gccgcttaag cattttagcc accgtcggtt gcgaaactcc
    25261 cagacgagca gccatgtcca cctgacgagc ttcccccact tccctgatca agtcagaaat
    25321 cagctcaacg taatcatcaa taagctcgcg ccgatgcgcc tctctgacct ggcggaaccc
    25381 ttcaacgtgc tcttccacgt tcactaactg cgtcactttt tttgctgttg gcgtacctgc
    25441 gcgacgactc attgtgcttc ctcattatgg tgacgcatac atagcgttct catttcgtgc
    25501 gcacattgta aaccagagtt gcgaaggtac aaaaaattaa cgttttagca atagctacat
    25561 aatatagcct gtgctatatc tgtatgtaat gcaatcatcc ctcaaggatc gacgggatta
    25621 gcaagtcagg aggtcttatg aatgagttca agaagtgtat gcgcgtgttt agtcattctc
    25681 cctttaaagt acggttaatg ctgctctcta tgttgtgcga tatggtcaac aacaaaccgc
    25741 agcaagataa accttccgat aaatagcggc gtcgcggtac gccgcttcac tcctgctttt
    25801 atgcaggcat aacgcgtttt ggtctgaaaa accccacttt ttgtcggatt tgcaatcccc
    25861 ttcgcaaaag atttgttcgt cagtagttga cctgaacggc ggctcgctct atcttcttgc
    25921 agccctgcgt atattgcggc tcgcggatgc ggaccccttt cccctcttca cgcactcttg
    25981 caggtattga cccttgacgc cagggtaagc acatggcgtt tgttacgata gtggcatatg
    26041 aatttaaccc tcaaagaatc gcttgttacc cgtagccggg tatttagccc gtggactgcg
    26101 ttctactttt tacagtcgct attaattaac ctcggcttag gttacccctt cagtttgctc
    26161 tacaccgctg cgtttacggc tattttgctt ttgctatggc gaacattgcc tcgcgtacaa
    26221 aaagttctgg tcggtgtcag ttcgctggtg gcggcttgtt atttcccttt tgctcaggcc
    26281 tacggcgcgc ctaatttcaa tacattgctg gcattgcact ccaccaatat ggaagagtcg
    26341 accgaaatcc tgacgatttt tccgtggtac agctacctgg tcggcttatt tatttttgcg
    26401 ctcggcgtaa tagcaatcag gcgaaaaaaa gagaatgaaa aagcgcgctg gaataccttc
    26461 gacagcctgt gtctggtatt cagtgtggcg acattttttg ttgctcccgt gcaaaacctg
    26521 gcctggggtg gcgtatttaa actgaaagat actggctatc cggtatttcg ttttgctaag
    26581 gatgtcatcg tcaataataa cgaggtgatt gaagagcaag aacggatggc aaaactttcc
    26641 ggaatgaaag atacctggac ggtcactgcc gttaagccga agtatcagac ctatgtggtg
    26701 gtgatcggcg aaagcgcgcg tcgcgatgcc ctcggtgcct ttggcggtca ctgggacaat
    26761 accccgtttg ccagcagcgt taacggtttg atatttgctg actacattgc cgccagtggc
    26821 tccacgcaga aatcacttgg cttaacgctc aatcgcgtag tcgatggcaa accacagttt
    26881 caggataact ttgtcaccct ggcaaatcgc gcgggcttcc agacctggtg gttttccaac
    26941 cagggccaaa tcggcgaata cgatactgct atcgccagca tcgccaaacg tgcagatgaa
    27001 gtgtacttcc tgaaagaagg taattttgaa gcagataaaa acacgaaaga cgaagcgtta
    27061 ctggatatga ccgctcaagt gctggcgcaa gagcactcgc aaccgcagct gattgttctg
    27121 catctgatgg gctcgcatcc gcaggcctgc gacaggacac aaggcaaata cgaaaccttt
    27181 gtgcaatcga aagaaacgtc gtgctatctc tataccatga cgcaaacgga cgatttactg
    27241 cgcaagctgt acgatcagtt acgcaacagc ggcagcagct tctctctggt ttacttttct
    27301 gaccacggtc tggcctttaa agagcgcggc aaagacgtgc aataccttgc ccatgatgat
    27361 aaatatcagc aaaatttcca ggtgcctttt atggtcattt ccagcgacga taaagcgcat
    27421 cgggtgatta aagcccgccg ctcagccaat gacttcttag gctttttctc gcagtggacg
    27481 ggaattaaag cgaaggaaat aaatatcaaa tacccgttta tatctgagaa gaaagccggg
    27541 ccgatataca tcaccaactt ccagttacag aaggtggatt acaaccatct cggaaccgat
    27601 attttcgacc cgaaacctta aaacaaaaaa tccgccccga gaggcggatt ttttatatca
    27661 ccaaagtgat tagaagcggt aaccaacacc ggcaatccag gtgcctacgt caacgctacg
    27721 aatacggctc tgctcgtaag agaagtccag agcaacgttt tccatcgggt tgaactgcag
    27781 accagcaccg taggagaaac cgtagtcgct ggtgtcgtgt ttgtaggtcg ggtattcagt
    27841 ggtctggaat ttaccataac ccacccccac tacaccgtag atgcttgccc agtcgttaat
    27901 gcggtaagcc ggaccagcag tgatgccgta gtactggttt ttgttgtagt caccagagct
    27961 tgcagtacgg cttttctcgg tgtaagtgaa agaaccgatc acacccagcg ggctgttgtc
    28021 ttcttcatag cggtatttca ggttgaaacc gcccattttg ttcatttggc cctgagcgtc
    28081 gctctgtgcg taaccgccag ttacggtaga agtcgcagct acggaagtac ctgcggtgaa
    28141 agccagaact gcggccagtg ctgaaagaca tgcaattttt ttcataacca cctcaaatgt
    28201 gattcaaata agttctaagt tttaaatata tcaaaaatta atgggaaact cttcgcgatt
    28261 tgtgatgtct aacgggccat ttcatgtaac agaacgtttc catacaccgc tatccatcta
    28321 aatttaaatc actttttcag agaactgcgt aagtattacg catgttttcc ctgtcattca
    28381 tccagattat tcctaatcac cagactaatg attccatcaa tcctggcgca ttttagtcaa
    28441 aacgggggaa aattttttca acaaatgctc aaccagcatt gggtatatcc agtacactcc
    28501 acgctttact taagtctaga tatttgtggg agaaaggatg cctggttcat tacgtaaaat
    28561 gccggtctgg ttaccaatag tcatattgct cgttgccatg gcgtctattc agggtggagc
    28621 ctcgttagct aagtcacttt ttcctctggt gggcgcaccg ggtgtcactg cgctgcgtct
    28681 ggcattagga acgctgatcc tcatcgcgtt ctttaagcca tggcgactgc gctttgccaa
    28741 agagcaacgg ttaccgctgt tgttttacgg cgtttcgctg ggtgggatga attatctttt
    28801 ttatctttct attcagacag taccgctggg tattgcggtg gcgctggagt tcaccggacc
    28861 actggcggtg gcgctgttct cttctcgtcg cccggtagat ttcgtctggg ttgtgctggc
    28921 ggttcttggt ctgtggttcc tgctaccgct ggggcaagac gtttcccatg tcgatttaac
    28981 cggctgtgcg ctggcactgg gggccggggc ttgttgggct atttacattt taagtgggca
    29041 acgcgcagga gcggaacatg gccctgcgac ggtggcaatt ggttcgttga ttgcagcgtt
    29101 aattttcgtg ccaattggag cgcttcaggc tggtgaagca ctctggcact ggtcggttat
    29161 tccattgggt ctggctgtcg ctattctctc gaccgctctg ccttattcgc tggaaatgat
    29221 tgccctcacc cgtttgccaa cacggacatt tggtacgctg atgagcatgg aaccggcgct
    29281 ggctgccgtt tccgggatga ttttcctcgg agaaacactg acacccatac agctgctggc
    29341 gctcggcgct atcatcgccg cttcaatggg gtctacgctg acagtacgca aagagagcaa
    29401 aataaaagaa ttagacatta attaaattta catttctgca tggttatgca taaccatgca
    29461 gaatttctcg ctacttttcc tctacaccgt ctttatatat cgaattatgc aaaagcatat
    29521 ttattccgaa aattcctggc gagcagataa ataagaattg ttcttatcaa tatatctaac
    29581 tcattgaatc tttattagtt ttgtttttca cgcttgttac cactattagt gtgataggaa
    29641 cagccagaat agcggaacac atagccggtg ctatacttag tctcgttaat tactgggaca
    29701 taacatcaag aggatatgaa attatgagta ccgctaaatt agttaaatca aaagcgacca
    29761 atctgcttta tacccgcaac gatgtctccg acagcgagaa aaaagcaaca gtagagttgc
    29821 tgaatcgcca ggttatccag tttattgatc tttctttgat taccaaacaa gcgcactgga
    29881 acatgcgcgg cgctaacttc attgccgtac atgaaatgct ggatggcttc cgcaccgcac
    29941 tgatcgatca tctggatacc atggcagaac gtgcagtgca gctgggcggt gtagctctgg
    30001 ggaccactca agttatcaac agcaaaaccc cgctgaaaag ttacccgctg gacatccaca
    30061 acgttcagga tcacctgaaa gaactggctg accgttacgc aatcgtcgct aatgacgtac
    30121 gcaaagcgat tggcgaagcg aaagatgacg acaccgcaga tatcctgacc gccgcgtctc
    30181 gcgacctgga taaattcctg tggtttatcg agtctaacat cgaataaatc catcgctgat
    30241 ggtgcagaac tttagtaccc gataaaagcg gcttcctgac aggaggccgt tttgttttgc
    30301 agcccacctc aacgcactta tttagtgcat ccatctgcta tctccagctg attaagtaaa
    30361 ttttttgtat ccacatcatc acacaatcgt tacataaaga ttgttttttc atcaggtttt
    30421 acgctaaata atcactgtgt tgagtgcaca attttagcgc accagattgg tgccccagaa
    30481 tggtgcatct tcagggtatt gccctataaa tcgtgcatca cgtttttgcc gcatctcgaa
    30541 aaatcaagga gttgcaaaac tggcacgatt ttttcatata tgtgaatgtc acgcagggga
    30601 tcgtcccgtg gatagaaaaa aggaaatgct atgaagtctg tattaaaagt ttcactggct
    30661 gcactgaccc tggcttttgc ggtttcttct catgccgcgg ataaaaaatt agttgtcgcg
    30721 acggataccg ccttcgttcc gtttgaattt aaacagggcg ataaatatgt gggctttgac
    30781 gttgatctgt gggctgccat cgctaaagag ctgaagctgg attacgaact gaagccgatg
    30841 gatttcagtg ggatcattcc ggcactgcaa accaaaaacg tcgatctggc gctggcgggc
    30901 attaccatca ccgacgagcg taaaaaagcg atcgatttct ctgacggcta ctacaaaagc
    30961 ggcctgttag tgatggtgaa agctaacaat aacgatgtga aaagcgtgaa agatctcgac
    31021 gggaaagtgg ttgctgttaa gagcggtact ggctccgttg attacgcgaa agcaaacatc
    31081 aaaactaaag atctgcgtca gttcccgaac atcgataacg cctatatgga actgggcacc
    31141 aaccgcgcag acgccgttct gcacgatacg ccaaacattc tgtacttcat caaaaccgcc
    31201 ggtaacggtc agttcaaagc ggtaggtgac tctctggaag cgcagcaata cggtattgcg
    31261 ttcccgaaag gtagcgacga gctgcgtgac aaagtcaacg gcgcgttgaa aaccctgcgc
    31321 gagaacggaa cttacaacga aatctacaaa aaatggttcg gtactgaacc gaaataataa
    31381 cgctacacct gtaaagcgca ctggcagttc cctctcccct ctggggagag gattagggtg
    31441 aggggcgcaa acccgctccg gggccattaa ttaccctgaa tttgattatt tacaccacgg
    31501 taacaggaac aacatatgca gtttgactgg agtgccatct ggcctgccat tccgcttctg
    31561 attgaaggtg ccaaaatgac cctgtggatt tcggtcctcg gtctggctgg cggtctggta
    31621 atcggattgc tggcaggttt tgcacgcacc ttcggtggtt ggatagccaa ccacgtcgcg
    31681 ctggtcttta ttgaggtgat ccgcggcaca cctatcgtcg tccaggtgat gtttatttat
    31741 ttcgccctgc cgatggcgtt taacgactta cgcatcgacc catttactgc ggcggtggtc
    31801 accatcatga tcaactccgg cgcgtacatt gcggaaatca cgcgtggtgc ggtgctgtct
    31861 attcacaaag gttttcgtga agcaggactg gcgctcggtc tttcacgttg ggaaaccatt
    31921 cgctacgtca ttttaccgct ggcactgcgt cgtatgctgc cgccgctggg taaccagtgg
    31981 atcatcagca ttaaagacac ctcgctgttt attgtgatcg gcgtggcgga actgacccgt
    32041 caggggcaag aaattattgc cggtaacttc cgcgcccttg agatctggag cgccgtggcg
    32101 gtgttctatc tgattattac cctggtgcta agctttattt tgcgtcgtct ggaaagaagg
    32161 atgaaaatcc tgtgattgaa tttaaaaacg tctccaagca ctttggccca acccaggtgc
    32221 ttcacaatat cgatttgaac attgcccagg gcgaagtcgt ggtgattatc gggccgtccg
    32281 gttccggtaa atcgaccctg ctgcgctgca tcaacaaact ggaagaaatc acctccggcg
    32341 atctgattgt cgatggcctg aaggttaacg atccgaaagt tgacgagcgc ctgattcgcc
    32401 aggaagcagg tatggtgttc cagcagtttt acctcttccc gcatctgacg gcgctggaaa
    32461 acgtcatgtt tggtccgcta cgcgtgcgtg gcgcgaacaa agaagaggcg gaaaaactgg
    32521 cgcgtgagct gctggcgaaa gtcggtctgg cagaacgtgc acatcactac ccttccgaac
    32581 tttctggtgg tcaacagcag cgtgtggcga ttgcccgcgc gctggcggtg aagccgaaaa
    32641 tgatgctgtt tgatgaaccg acttccgctc ttgacccgga actgcgccat gaagtgctga
    32701 aggttatgca ggatctggct gaagaaggga tgacgatggt gatcgtgacc cacgaaatcg
    32761 gttttgccga gaaagtagct tcgcggctga tctttatcga caaaggccgg attgcggaag
    32821 atggcgatcc gcaggtattg atcaagaacc cgccgagcca gcgcttgcag gaatttttgc
    32881 agcacgtctc ttaacttctc tgcctccctt tcacccgaaa gggaggcaca ccagattcct
    32941 ctcatttaaa atcgcccctc ccccagcatc tatacttatc tttttgctct gttttctcac
    33001 tggaggagtc atgcggtgga tcctgttcat cctcttctgc ctgctgggcg cacctgccca
    33061 cgcggtatcc atacccggcg ttacaaccac aacgacaacg gactcaacga ctgaaccggc
    33121 cccggaaccg gatatcgaac aaaaaaaagc ggcctatggc gcactggcgg atgtgctgga
    33181 taacgacacc tcgcgtaaag aattgatcga ccagttgcgt actgtcgccg cgacgccccc
    33241 tgcggaaccg gtcccgaaga tcgtgccgcc gacgctggtt gaagagcaaa ccgtgctgca
    33301 aaaggtcacc gaagtcagcc gccattatgg tgaagccctt tccgcccgct tcgggcaact
    33361 ttatcgcaat atcaccggct ccccgcataa gccatttaat ccacaaacct tcagcaatgc
    33421 gctgagccat ttttcaatgt tagcggtatt agtgtttggt ttttactggc tgattcgcct
    33481 gtgcgcactg ccgctgtatc gcaaaatggg tcagtgggcg aggcaaaaaa atcgtgagcg
    33541 cagcaactgg ttgcagcttc cggcgatgat tatcggggcg tttattatcg acctgctgtt
    33601 actggccctg acattatttg tcggccaggt attaagcgac aacctgaatg caggcagtcg
    33661 caccatcgct ttccaacaaa gtttgtttct caacgccttt gccctgattg aatttttcaa
    33721 agccgtacta cgcctgattt tttgcccaaa cgtggcggag ctgcgcccgt tcacgattca
    33781 ggacgagagc gcccgttact ggagtcgtcg cctgagctgg ttaagcagtt tgataggtta
    33841 tggcctgatt gtggccgtgc cgattatctc taatcaggtg aatgtacaga taggtgcgct
    33901 ggcgaacgtc atcattatgc tgtgcatgac cgtctgggcg ttgtacctga tctttcgtaa
    33961 taaaaaagag attacccagc atttgctcaa ctttgcggag cattcgctgg cctttttcag
    34021 cctgtttatc cgcgcctttg cgctggtgtg gcactggctg gcaagcgcct attttatcgt
    34081 gctgtttttc ttttcgttgt tcgatccggg caacagcctg aaatttatga tgggtgcaac
    34141 ggtgcgcagc ctggcgatta ttggtatcgc agcgtttgtt tccggtatgt tttcccgctg
    34201 gctggcaaaa accatcactc tctcgccaca tactcagcgt aactatccag agttgcaaaa
    34261 acggctgaat ggctggcttt cggcggcgct gaaaacggcg cgtattctga ccgtctgcgt
    34321 ggcggtaatg ttgctgttga gcgcatgggg attgttcgat ttctggaact ggctgcaaaa
    34381 cggcgcgggg cagaaaaccg tagatatcct gatccgtatc gcacttattc tttttttctc
    34441 ggcggttggc tggacggtgc tcgccagttt gatcgaaaac cggctggctt cggatattca
    34501 tggccgcccg ctacccagcg cccgtacgcg taccctgctg acgctgttcc gtaacgcgct
    34561 ggcggtgatt atcagtacca tcaccatcat gattgtgttg tcggaaatcg gcgtcaatat
    34621 cgcgccattg ctggcaggtg ccggggcatt aggtctggct atctcgtttg gttcgcaaac
    34681 gctggtgaaa gatatcatta ccggggtgtt tattcagttt gaaaacggca tgaacactgg
    34741 agatttggtg actatcgggc cgttgaccgg cacagtggaa cggatgtcga ttcgctccgt
    34801 gggcgtgcgc caggataccg gggcgtatca catcattccg tggtcttcga taaccacctt
    34861 tgctaacttc gtccgcggta ttggttcggt agtggcaaac tacgatgttg atcgccatga
    34921 agatgctgat aaagccaatc aggcactgaa agatgcggta gcggaattaa tggaaaacga
    34981 agaaattcgc gggctgatta ttggtgaacc gaattttgcc gggattgtcg gcttaagcaa
    35041 taccgcgttt acactgcgtg tttcgttcac cacgctgcca ctcaaacagt ggacggtacg
    35101 ctttgccctc gacagccagg tgaaaaaaca tttcgacctg gcgggcgttc gcgcgccagt
    35161 gcagacttat caggtgctgc ctactccggg cgcgaccccg gctgaaccgt taccgccggg
    35221 ggagccaacg ctttaacgct ggcgattgac aaaacggcgg cgctgctcgt cgttcataaa
    35281 ggtccaggca ataaagcgac tctgcttttg cccctgggcc atctcttttt ttaccacctt
    35341 caccgcgccc acgtcggtca gagcacgata caacggcggt aagttttcac cacgagatac
    35401 cagcgaggta aaccacatca cctgcttcgc gaagcctttg ctctcttcaa tcatcttttt
    35461 gataaaggcg acttcaccac cttcacacca caactcctgt tgctgaccac caaagttcag
    35521 tgcatcgtct ttgttcagcc ccaggttacg gcgtttacgc tcactacctg cccgcgctgc
    35581 agcggcggaa tcgtggaatg gcgggttaca caaggtcgcg tcgtattgct cgtttttatg
    35641 gatgatgccg ttaaaaattg ccccactctc tttttgccga cgcagacgaa tggcgcggtt
    35701 aagccccgga ttagcactga taatcgcctg cgcactgctt aacgcctggc tgctggtttc
    35761 actaccggta aaacgccagc catattcatg tacgccaatc agcggataaa tacagttcgc
    35821 accaacgccg atatccagaa tgctggcatt cgctggaatt gttccgcttg cttcagccag
    35881 taaatcggca aggtgatgaa tataatccgc ccggcccggt actggtgggc agagaaaacc
    35941 gtcggggata tcccagttcg ctacggcgta aaaatgggcc agcaacgcct tattgagcgc
    36001 cttcaccgcc agcggattgg caaagtctac gctttgctcc ccggcgggtg taagcgtgag
    36061 gaattgcctg agttcaggat tgacctgaca aagcgtggcg agatcgtagc ggctgtgatg
    36121 acggttgcgc ggatgcaacc ccggtttctg ggcggacata gcattctcct ttaagcagcg
    36181 gcgtaagata cccgcataac gcgacagggt aaatatctgg ctgcgtttat cattccccaa
    36241 aaccttaaca tggctatggt ttatggcaat tcatcataaa aatcagcatt atgcatgtca
    36301 ttaatttgtc ttattttgta aaaatatatc gttcaaaaaa taagcacatc tctgtcaatt
    36361 ttttgcgtca tttgcatcgc gcaggcgacc tacacttatc aggcactacc cgttgtattt
    36421 atgaggatgt cattatgaaa aagtgcctca cactactgat tgccaccgtc ctgagcggaa
    36481 tctctctcac ggcttatgcc gcgcaaccga tgagtaacct ggacagcggt cagctacggc
    36541 ccgccggtac cgtttcggca actggcgcat caaacctaag cgatctggag gataaactgg
    36601 cagaaaaagc gcgcgaacaa ggcgcgaaag gttatgtcat taattccgca ggcggaaatg
    36661 accagatgtt cggtactgca accatctaca aataacccca ccaacgtaac tgcattcgcc
    36721 tctggatgca gttttcgcct gttttgtaat caaaaaacat caaatatgaa ctcaatgtaa
    36781 ataaatgtat ttctttttcg cgcaatgggt gatagaaaat cgcgccaagt gataatgctt
    36841 atcaaaatta ttatcacttg cacgaacact atcacgggat taacagtggc atcgcatccg
    36901 cagagatgct ttctcgtggc agtgaaaatt tcaacatata agaaaaagtc acctgcaaaa
    36961 tggaaaacaa tcgcaatttc cctgccagac aatttcattc gctcacgttc tttgccggtc
    37021 tttgtattgg catcacgcct gtggctcagg cactcgccgc cgaagggcaa actaacgcgg
    37081 atgacacgct ggttgtcgaa gcatcaacgc cttcgcttta tgcgccacaa caatctgccg
    37141 atccgaaatt ctcgcgtccg gtagcggata ctacccgcac gatgacggtg atttctgaac
    37201 aagtgattaa agatcagggc gcaaccaacc ttaccgacgc gctcaaaaac gcccccggcg
//
LOCUS       DAZPDU010000023        36747 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZPDU010000023 DAZPDU010000000
VERSION     DAZPDU010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36747
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.023"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..135)
                     /gene="tabA"
                     /locus_tag="ACHURS_001034"
     CDS             complement(<1..135)
                     /gene="tabA"
                     /locus_tag="ACHURS_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFR1236826.1"
                     /translation="MIIGNIHNLQPWLPQELRLAIEHIKAHVTAETPKGKHDIEGNRL
                     F"
     gene            complement(280..873)
                     /locus_tag="ACHURS_001035"
     CDS             complement(280..873)
                     /locus_tag="ACHURS_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFR1236827.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            944..1657
                     /gene="bdcA"
                     /locus_tag="ACHURS_001036"
     CDS             944..1657
                     /gene="bdcA"
                     /locus_tag="ACHURS_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR1236828.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     EHLAQKTGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMAREL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            1788..2183
                     /locus_tag="ACHURS_001037"
     CDS             1788..2183
                     /locus_tag="ACHURS_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFR1236829.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            2464..2598
                     /gene="pyrL"
                     /locus_tag="ACHURS_001038"
     CDS             2464..2598
                     /gene="pyrL"
                     /locus_tag="ACHURS_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFR1236830.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            2602..3537
                     /gene="pyrB"
                     /locus_tag="ACHURS_001039"
     CDS             2602..3537
                     /gene="pyrB"
                     /locus_tag="ACHURS_001039"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFR1236831.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            3550..4011
                     /gene="pyrI"
                     /locus_tag="ACHURS_001040"
     CDS             3550..4011
                     /gene="pyrI"
                     /locus_tag="ACHURS_001040"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFR1236832.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            4084..4470
                     /gene="ridA"
                     /locus_tag="ACHURS_001041"
     CDS             4084..4470
                     /gene="ridA"
                     /locus_tag="ACHURS_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFR1236833.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(4676..7372)
                     /gene="mgtA"
                     /locus_tag="ACHURS_001042"
     CDS             complement(4676..7372)
                     /gene="mgtA"
                     /locus_tag="ACHURS_001042"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFR1236834.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(7513..7566)
                     /gene="mgtL"
                     /locus_tag="ACHURS_001043"
     CDS             complement(7513..7566)
                     /gene="mgtL"
                     /locus_tag="ACHURS_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFR1236835.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            7751..8698
                     /gene="treR"
                     /locus_tag="ACHURS_001044"
     CDS             7751..8698
                     /gene="treR"
                     /locus_tag="ACHURS_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFR1236836.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            8817..10238
                     /gene="treB"
                     /locus_tag="ACHURS_001045"
     CDS             8817..10238
                     /gene="treB"
                     /locus_tag="ACHURS_001045"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFR1236837.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            10288..11943
                     /gene="treC"
                     /locus_tag="ACHURS_001046"
     CDS             10288..11943
                     /gene="treC"
                     /locus_tag="ACHURS_001046"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFR1236838.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            12337..14475
                     /gene="nrdD"
                     /locus_tag="ACHURS_001047"
     CDS             12337..14475
                     /gene="nrdD"
                     /locus_tag="ACHURS_001047"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFR1236839.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSRESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGCEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            14633..15097
                     /gene="nrdG"
                     /locus_tag="ACHURS_001048"
     CDS             14633..15097
                     /gene="nrdG"
                     /locus_tag="ACHURS_001048"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFR1236840.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(15142..15528)
                     /gene="cybC"
                     /locus_tag="ACHURS_001049"
     CDS             complement(15142..15528)
                     /gene="cybC"
                     /locus_tag="ACHURS_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFR1236841.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     ATQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(15712..17064)
                     /gene="pmbA"
                     /locus_tag="ACHURS_001050"
     CDS             complement(15712..17064)
                     /gene="pmbA"
                     /locus_tag="ACHURS_001050"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFR1236842.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGLADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLLPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            17158..17709
                     /gene="yjgA"
                     /locus_tag="ACHURS_001051"
     CDS             17158..17709
                     /gene="yjgA"
                     /locus_tag="ACHURS_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFR1236843.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(17792..19165)
                     /gene="mpl"
                     /locus_tag="ACHURS_001052"
     CDS             complement(17792..19165)
                     /gene="mpl"
                     /locus_tag="ACHURS_001052"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFR1236844.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVASADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            19341..20339
                     /gene="fbp"
                     /locus_tag="ACHURS_001053"
     CDS             19341..20339
                     /gene="fbp"
                     /locus_tag="ACHURS_001053"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFR1236845.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENAQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEY
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(20372..21367)
                     /gene="yjfF"
                     /locus_tag="ACHURS_001054"
     CDS             complement(20372..21367)
                     /gene="yjfF"
                     /locus_tag="ACHURS_001054"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFR1236846.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(21354..22376)
                     /gene="ytfT"
                     /locus_tag="ACHURS_001055"
     CDS             complement(21354..22376)
                     /gene="ytfT"
                     /locus_tag="ACHURS_001055"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFR1236847.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSGVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            complement(22390..23892)
                     /gene="ytfR"
                     /locus_tag="ACHURS_001056"
     CDS             complement(22390..23892)
                     /gene="ytfR"
                     /locus_tag="ACHURS_001056"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFR1236848.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFFLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHVLQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVDYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(24032..24988)
                     /gene="ytfQ"
                     /locus_tag="ACHURS_001057"
     CDS             complement(24032..24988)
                     /gene="ytfQ"
                     /locus_tag="ACHURS_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFR1236849.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTGGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMMDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            25298..25828
                     /gene="ppa"
                     /locus_tag="ACHURS_001058"
     CDS             25298..25828
                     /gene="ppa"
                     /locus_tag="ACHURS_001058"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFR1236850.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(26207..26548)
                     /locus_tag="ACHURS_001059"
     CDS             complement(26207..26548)
                     /locus_tag="ACHURS_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFR1236851.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(26551..30330)
                     /gene="tamB"
                     /locus_tag="ACHURS_001060"
     CDS             complement(26551..30330)
                     /gene="tamB"
                     /locus_tag="ACHURS_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFR1236852.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGNVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDIILNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(30327..32060)
                     /gene="tamA"
                     /locus_tag="ACHURS_001061"
     CDS             complement(30327..32060)
                     /gene="tamA"
                     /locus_tag="ACHURS_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFR1236853.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSDWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGGGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            32266..32904
                     /gene="msrA"
                     /locus_tag="ACHURS_001062"
     CDS             32266..32904
                     /gene="msrA"
                     /locus_tag="ACHURS_001062"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFR1236854.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            33227..34570
                     /locus_tag="ACHURS_001063"
     CDS             33227..34570
                     /locus_tag="ACHURS_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFR1236855.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(34667..34873)
                     /locus_tag="ACHURS_001064"
     CDS             complement(34667..34873)
                     /locus_tag="ACHURS_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFR1236856.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            35198..35752
                     /locus_tag="ACHURS_001065"
     CDS             35198..35752
                     /locus_tag="ACHURS_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFR1236857.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            complement(35842..>36747)
                     /gene="ytfI"
                     /locus_tag="ACHURS_001066"
     CDS             complement(35842..>36747)
                     /gene="ytfI"
                     /locus_tag="ACHURS_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtfI"
                     /protein_id="HFR1236858.1"
                     /translation="PRIRHNNFIGAVELFVKSSYTKTHSNNFFNNIQHAFKKKDWISN
                     YDSLLTLREFFRCATQIDKNSYQVLSSKNGTVNAMDKFLISFSLKDNGAEYTMTLRGS
                     GFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIHQAI
                     GDVSLGYALLEDIVSKLKRGKFELQIPGGGIKECDGWYIHEKIIDDNFAIVIESLGFA
                     LKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADSNI
                     TKEKICEKYKSTIEAFKKEQLALPV"
BASE COUNT         8898 a         9846 c         9136 g         8867 t
ORIGIN      
        1 aaacagacga ttgccttcga tatcgtgctt gccctttggc gtttctgccg taacgtgtgc
       61 tttgatatgc tcaatcgcca ggcgtaactc ctggggtagc cacggctgaa gattatgaat
      121 atttccgatg atcatctttt ccactcctta attaaaacaa tgtttcattt ccatcgttat
      181 acgaaaagat gatcaattcc gctagcctta atgcgtgaat tattgtctta tcacaactta
      241 tcggcaggcg cgttcccgcc tttcgggtca aggcccgcat cactccttga gaatagtttt
      301 tatcgcttcc cccgccagtg cagctgttgc acagagttgt tcgatggagt gcccctcccg
      361 agccttcgca gaaagccctg acatcacggt actcataaaa tcagtcacac attgtgcgct
      421 ttgtggatgc cgcctggcga tatagtcata aatggccgtt tctgcggcgt gataatattg
      481 aacggcaata tcacgcgctt gtggatcatg actatgaatg ccttcaagaa ccatacagcc
      541 agcgcagccg ccgttttggc tatatctgcg cgccgcttct tttaatacct cagccaggca
      601 ctcccctacc ggacgatcat cacgaagaat atcggcaagc ggaatagctt ccgtaccgac
      661 atattcattg agtacacggc taaataaccc agccttactg ccaaaagccg cgtagaggct
      721 cggggggtta ataccaagat aatcagtaac ttcagcaaca ctgacagcat cgaaaccttt
      781 ttggtgaaaa agcacttttg ccgcagagac tgcctgctca ggagcgaaac gtctgggacg
      841 acctggaacg cgagattgtt ttttagtgac cattacaaaa cttgttgaca gaaagttaaa
      901 acagttttgt aatgcatgtt acataataaa tcaaggagtc cttatgggcg cttttacagg
      961 taagacagtt ctcatcctcg gtggcagtcg tggtatcggt gccgctatcg tacgtcgttt
     1021 cgtcaccgat ggggccaatg tacgattcac ctatgcgggg tcgaaagatg ccgctgaaca
     1081 cctggcacaa aagactggag cgacagcagt attcacagat agtgctgaca gagacgctgt
     1141 cattgatgtc gttcgtaaga gcggcgcatt ggatatcctg gtggtaaatg caggtattgg
     1201 cgtctttggc gatgccctgg aattgaatgc cgacgatatt gatcgccttt tcaaaatcaa
     1261 tattcatgct ccttatcatg cctctgttga agccgcccgg cagatgcccg aaggcgggcg
     1321 catcttaatc atcggctccg tgaatggcga tcgtatgcct gttgcaggca tggctgctta
     1381 tgccgccagt aaatctgccc tgcaaggcat ggcgcgcgag ctggcccgtg atttcggacc
     1441 gcgtgggatc accatcaacg tcgtccagcc agggccaatt gataccgacg ctaatcccgc
     1501 caacgggcca atgcgcgata tgttgcatag ttttatggct atcaaaagac atgggcaacc
     1561 ggaagaggtc gctggtatgg tcgcatggtt agcagggcca gaagccagtt ttgttaccgg
     1621 cgcgatgcat accattgatg gcgcgtttgg cgcataaccg actacgctca attaagccca
     1681 gccatttccc atgatgtctg ggttttgttt actcacgtcg tccgctaaaa gcggctcctg
     1741 gtaaatataa atcttctgtt catgtcgtaa atcttgagga taaccaaatg gtagaaagaa
     1801 ccgctgtttt ccctgcaggc cgacattcac tgtatgctga acatcgttat tctgcggcta
     1861 ttcgttccgg cgatttgctg tttgtttccg ggcaagtagg aagtcgagag gacggaacac
     1921 cagaacccga ttttcagcaa caagtcagac tggcatttga taatttgcat gcgaccctgg
     1981 ctgctgcggg atgcactttt gacgatatca ttgatgttac gagcttccat accgatccag
     2041 aaaaccaatt tgaagacatc atgacggtga aaaatgaaat atttagcgcc ccaccttatc
     2101 caaactggac ggcggtaggt gttacatggc tggcaggctt tgattttgaa attaaagtga
     2161 tagcgcgcat ccctgagcag taagcaatag tgttagccgt tcgttttcac actccgccct
     2221 ataagtcgga tgaatggaat aaaatgcata tctgattgcg tgaaagtgaa aaaggaaaaa
     2281 gcaggaaatg tctgcaatta ttgataccga acgaccgttc ccctgcagaa tcacaacaaa
     2341 taaaaatgca tgtattttga cttataattc aaataaaccg tttgcgctga caaaatattg
     2401 catcaaatgc ttgcaccgct tctgacgatg agtataatgc cggacaattt gccgggagga
     2461 tgtatggttc agtgtgttcg acattttgtc ttaccgcgtc tgaaaaaaga cgctggcctg
     2521 ccgtttttct tcccgttgat cacccattcc cagcccctca atcgaggggc ttttttttgc
     2581 ccaggcgtca ggagataaaa gatggctaat ccgctatatc agaaacatat catttccata
     2641 aacgacctta gtcgcgatga ccttaatctg gtgctggcga cagcggcgaa actgaaagca
     2701 aacccgcaac cagagctgtt gaagcacaaa gtcattgcca gctgtttctt cgaagcctct
     2761 acccgtaccc gcctctcttt cgaaacttcc atgcaccgcc tgggtgccag cgtggtgggc
     2821 ttctccgaca gcgccaatac atcactgggt aaaaagggcg aaacgctggc cgataccatt
     2881 tcggttatca gcacttacgt cgatgcgata gtgatgcgtc atccgcagga aggtgcggcg
     2941 cgcctggcca ccgagttttc cggcaatgta ccggtactga atgccggtga tggctccaac
     3001 caacatccga cgcaaacctt gctggactta ttcactattc aggaaaccca ggggcgtctg
     3061 gacaatctcc acgtcgcaat ggttggtgac ctgaaatatg gccgcaccgt tcactccctg
     3121 actcaggcgt tagcgaagtt cgacggcaac cgtttttact tcatcgcgcc ggacgcgctg
     3181 gcaatgccgc aatacattct ggatatgctt gatgaaaaag ggatcgcctg gagtctgcac
     3241 agctctattg aagaagtgat ggcggaagta gacatcctgt acatgacccg cgtgcaaaaa
     3301 gagcgtctgg acccgtccga gtacgccaac gtgaaagcgc agtttgttct tcgcgccagc
     3361 gatctccaca acgccaaagc caatatgaaa gtgctgcatc cgctgccgcg tgttgatgag
     3421 attgcgacgg atgttgataa aacgccacac gcctggtact tccagcaggc aggcaacggg
     3481 attttcgctc gccaggcgtt actggcactg gttctgaatc gcgatctggt actgtaaggg
     3541 gaaatagaga tgacacacga taataaattg caggttgaag ctattaaacg cggcacggta
     3601 attgaccata tccccgccca gatcggtttt aagctgttga gtctgttcaa gctgaccgaa
     3661 acggatcagc gcatcaccat tggtctgaac ctgccttctg gcgagatggg ccgcaaagat
     3721 ctgatcaaaa tcgaaaatac ctttttgagt gaagatcagg tagatcaact ggcattgtat
     3781 gcgccgcaag ccacggttaa ccgtattgac aactatgaag tggtgggtaa atcacgcccc
     3841 agtctgccgg agcgcatcga caatgtgctg gtctgcccga acagcaactg tatcagccat
     3901 gccgaaccgg tttcttccag ctttgccgtg cgaaaacgcg ccaatgacat cgcgctcaaa
     3961 tgcaaatact gtgaaaaaga gttttcccat aatgtggtgc tggccaatta attgcggttg
     4021 gtaataaaag tctggctccc tataatgagc cagacttttt accgctgtaa taaaggagaa
     4081 atcatgagca aaactatcgc gacggaaaat gcaccggcag ctatcggtcc ttacgtacag
     4141 ggcgttgatc tgggcaatat gatcatcacc tccggtcaga tcccggtaaa tccgaaaacg
     4201 ggcgaagtac cggcagacgt cgctgcacag gcacgtcagt cgctggataa cgtaaaagcg
     4261 atcgtcgaag ccgctggcct gaaagtgggc gacatcgtta aaaccaccgt gtttgtgaaa
     4321 gatctgaacg acttcgcaac cgttaatgct acctacgaag ccttcttcac cgaacacaat
     4381 gccaccttcc cggcacgttc ttgcgttgaa gttgcccgtc tgccgaaaga cgtgaaaatt
     4441 gagatcgaag cgatcgctgt tcgtcgctaa tcttgatgga aatccgggct atcatgcccg
     4501 gattaagtct gatgacaaac acaaaatcgc ctgatgcgct acgcttatca ggcctacgtg
     4561 attcctgcaa tttattgaat ttgttggccg gataaggcat ttacgccgca tccggcatga
     4621 acaaaactca ctttgtctgc aatctgaatc caggccacca tgcccagttg attctttatt
     4681 gccagccgta acgacggcta taaaatcctt tcaccagttg ggttaacgtc atataccctg
     4741 ccagaatcgc aaccagccac gggaaatagc ttaacggcag cgcctgcagt tgcagataac
     4801 tggccagcgg tgaaaacggc aatgcgatcc cgacaatcat cacgatcacg gtcatgatca
     4861 ttaacggcca cgatgcacag ctctgaataa acggcacacg gcgggtgcgg atcatatgca
     4921 caatcagcgt ttgcgacagc aagcccacca caaaccatcc cgactggaac agcgtttgcg
     4981 tttccggcgt gttggcatgg aatacccacc acatcaggca aaacgtcaga atatcgaaga
     5041 tcgagctgat cggtccgaag aagatcataa agcgccccag atccgccgga ttccaacgct
     5101 gcggcttttg aatttgctcg tcgtcgacgt tatcaaacgg gatcgccacc tgtgacacat
     5161 catacagcag gttctgaata agcaagtgta acggcaacat cggcaggaag ggcaagaaag
     5221 cactcgccac cagcacgctg aacacattac cgaagttgga gctcgccgtc attttgatgt
     5281 atttcagcat gttggcgaaa gtgcgacgtc cttcaataac cccctcttcc agcaccatca
     5341 ggcttttttc cagcaggatg atatcagccg cttcacgggc aatatctacc gcgccgtcca
     5401 cagaaatgcc gatatccgca gcgcgtaagg cgggcgcatc attaatacca tcgcccataa
     5461 agccaaccac atgcccttcg cgcttcagta aggtcacaat gcgttcttta tgcatcggcg
     5521 tcagacgagc aaacagcgtg gtgcgctgtg cgagatttgc cagttcgtcg tcagatagcg
     5581 tttcaatatc actaccaatg accacctctc ccgcgtccag ccccacttca tggcacactt
     5641 tcgctgcaac taactcactg tcgccagtaa ggatttttac ggtaattcca ctcgctttta
     5701 atgccttcag tgccggagca gttgtctctt ttggcggatc aaggaaagca atatatcctt
     5761 cgaggatcag gtcggattca tccgcccgct ggtaatctcc ttcacgcgct ggcaggtatt
     5821 tcgtcgccac cgcaaccacg cgcagcccct gacgattcag cgtatcagta acccgcttaa
     5881 tcttacgcag catgatgtca tcgagcggca caatctcgcc attgtgacgc acctgcgaac
     5941 acacattgag gatttcctgc aatgcacctt tgcaaaccag ctgatggtgc tcggtatttt
     6001 ctgccactac caccgacatc cggcgacgct cgaaatcaaa cggaatctca tcaatttttt
     6061 gccaacggct ggccagagag cgcgctgact cttcatccgt accttcgagc accgctgtat
     6121 cgagtaggtt tttaagtccg gtctgataat gactgttcaa ccacgcgcta tgcagcacgc
     6181 gttcgctggt tttaccggag atatcggtat gattctccag cacaattttg tcctgtgtca
     6241 gggtgccagt tttatcagtg cacagaatat ccattgcgcc aaagttctga atagcatcca
     6301 gatgtttgac gatcactttc tgtttcgaca gctttactgc gccgcgcgcc agcgtcgagg
     6361 taacaatcat cggcaacatt tccggcgtta agcctaccgc gaccgaaagc gcaaacagtg
     6421 ccgcttccca ccagtcgcct ttggtgtaac cgttgataag caacaccacc ggagccatca
     6481 ccagcataaa gcgaatcagc agcatactga cgcggctgat cccctgctga aaggcattcg
     6541 gctcgctttc ctgctcacta acacgccccg ccagttgacc aaaccaggtg ttggctcctg
     6601 tagcaatcac cattgcttgt gccgtgccgc tcaccacggt ggtgcccata aaacacaggg
     6661 tgtcacactc cagcggattg ctgtgctccg gctggcgagt ggtagcggct ttttccacgg
     6721 gcagagactc accggttaac gacgcctgag cgacgaacag atcccgcgcc tgcaagatac
     6781 gtaaatctgc cgggatcata tcccccgccg ccagttttat aatatcgccg ggcaccagct
     6841 ggtcgatcgg gatctccagc cagccatttt cgcctttgtc gttaattacg cgcagcaccg
     6901 tcgcggtatt gctgaccatc gctttcaggg catctgccgc tttagtggaa cgtgcttcct
     6961 gaataaagtt cagcaacgta gaaatagcga ccattagcgc gataacgccc gcggcaaata
     7021 aatcttcagt ggcgtaagaa atagcgccga gaatggtgag taaaatatta aatgggttgc
     7081 gatagcagac ccataaatgt acccaccacg gcgacggttg ttgtgcgggt aatttatttt
     7141 caccatgttg ttcgcgggca gattccactt ccgcctgatt taacccttcc ggatgggtgt
     7201 cgaacgtttt ccacaattct tcttcgggca tcaccgccat tttcaggcaa tgcgcactta
     7261 aggacggcgg gaccgccgta ttcactgtct gctgcgcgcc aggcaatgga tcgcgatgaa
     7321 ccagacggga aggtaaatgg cgaatgagcc gggtaaaaat ttctttaaac ataaggagtc
     7381 cctccgcact gtctgaaaca gtacagaaat gtcttcaggc gtggcgaagc cttacctgaa
     7441 ggtaataaag ctgagcaggg acgttaaaac gtcacggcct tacgcatccg gtagcgccgt
     7501 gaaaagacgg gcttaccgga aaagttttaa tctccgtcga gggagaggcg tgggatcagg
     7561 ttccatataa cctccggtaa gtgaaaaaag cgccgcaaat aataaggaga atccaataat
     7621 attcgtagca tttatggcga tataatactt gctggcaact aaaccaacga taaaccagac
     7681 tttacccatt gctgaatgca cgggtaaagt taggctcaaa taattaaaca acacgttaca
     7741 ggacaacagg atgcaaaatc ggctgactat caaagacatc gcacgcttaa gcggcgtggg
     7801 gaaatctaca gtttcccggg tgctgaataa cgaaagcggc gtgagccagc gcacccgcga
     7861 gcgtgttgaa gcagtgatga atcagcatgg attttcccct tcccgctctg cgcgcgctat
     7921 gcgtgggcaa agcgataaag tggtcgccat cattgttacc cgtctggatt cgttgtcaga
     7981 aaatctcgcc gttcaaacca tgctgccagc gttctatgaa caaggttacg acccaatcat
     8041 gatggaaagc cagttttccc cgcaattagt tgccgaacat ttgggggtgc tgaaacggcg
     8101 taatatcgac ggcgtagtgc tgttcggttt tactggcata acagaagaaa tgttagccca
     8161 ctggcagtca tcgctggttc tgctggcgcg tgacgcaaaa ggctttgctt cggtctgtta
     8221 tgacgacgaa ggggcaatca agatcctgat gcaacggctg tatgaccagg ggcatcgtaa
     8281 tatcagttat ctcggcgtgc cgcatagtga cgtgacaacc ggtaagcgac gtcacgaagc
     8341 ctacctggcg ttctgcaaag cgcataaact gcatcccgtt gccgctctgc cagggcttgc
     8401 tatgaagcaa ggctatgaga acgtggcaaa agtgattacg cctgaaacta ctgcgttact
     8461 gtgcgcaacc gacacgctgg cacttggcgc aagtaaatac ctgcaagagc aacgcatcga
     8521 caccttgcaa ctggcgagcg tcggtaatac accgttaatg aaattcctcc atccggagat
     8581 cgtcaccgta gatcccggtt acgccgaagc tggacgccag gcggcctgcc aattgatcgc
     8641 acaggtaacc gggcgcagcg aaccgcaaca aatcatcatc cccgccaccc tgtcctgatc
     8701 gtttcctgaa cgataaattg tgatcttcgc tgcatttcgg gaacgttccc gtttttaaat
     8761 ttttccgcgc aatatattct gcagccaacc aaaaatgtca tctgccatgg ggctttatga
     8821 tgagcaaaat aaaccaaacg gatatcgatc ggttgattga actggtcggc gggcgcggca
     8881 atattgcgac ggtgagccac tgtattactc gcctgcgctt tgtcctcaac caaccggcca
     8941 atgccagacc gaaagaaatt gagcaactcc ccatggtgaa aggctgtttc accaatgccg
     9001 ggcaatttca ggtggtgatt ggcaccaacg tgggtgatta ctatcaagca ctaatagcgt
     9061 caaccggaca ggcgcaggtt gataaagagc aggtaaaaaa agccgcccgg cagaatatga
     9121 aatggcatga gcagttgatc tctcatttcg cggagatctt cttcccgttg ctgcccgcgt
     9181 tgattagcgg cggtttgatc ctcggttttc gcaatgtgat cggcgatttg cccatgagca
     9241 acggtcagac gctggcgcaa atgtaccctt ccctgcaaac gatctacgat tttctgtggt
     9301 tgatcggtga agcgattttc ttctacctgc cggtcgggat ttgctggtca gcggtgaaaa
     9361 aaatgggcgg cacgccgatc cttggtatcg tgcttggcgt gacactggtt tccccccagc
     9421 tgatgaacgc ttatctgctc gggcagcagc tgccggaagt gtgggacttt ggcatgttca
     9481 gcatcgccaa agtgggctat caggcgcagg tgatcccggc actgttagcc gggctggcgc
     9541 tgggcgttat tgaaactcgc cttaaacgca tcgtaccgga ttacctctat ctggtggtgg
     9601 tgcccgtctg ttcgctgatc ctcgcggtgt tcctcgccca tgcgctgatt ggtccgtttg
     9661 gtcgcatgat tggcgatggc gttgcctttg cggtacgtca cctgatgacc ggcagctttg
     9721 ctccgattgg tgcggcattg tttggcttcc tgtacgcgcc gctggtgatc accggcgtac
     9781 accagaccac ccttgctatt gatttgcaga tgattcaaag catgggtggc acgccagtgt
     9841 ggccgctgat tgcgctgtcg aatatcgctc agggctccgc cgtgataggc attatcattt
     9901 ccagccgcaa gcacaatgaa cgcgagatct ccgtgcctgc cgctatctcc gcctggcttg
     9961 gggtcactga gcctgcaatg tacggcatca acctgaaata tcgcttcccg atgctgtgcg
    10021 cgatgattgg ttctggtctg gcaggattgc tatgcggcct gaacggcgtt atggcgaatg
    10081 gtatcggcgt agggggcctg ccgggaattc tctcgattca accgagctac tggcaggtat
    10141 ttgcgctggc aatggctatc gccatcatca tcccgattgt actcacctcg tttatctatc
    10201 agcggaaata ccgcttgggc acgctggaca ttgtttaatt ttcttcgggg cgcaattgcg
    10261 ccccctcgca tttgcaggaa taacgtaatg actaatcttc cccactggtg gcaaaacggc
    10321 gttatctacc agatttatcc aaagagtttt caggacacca cgggtagcgg caccggcgat
    10381 ttacgtggcg ttatccaacg cctggactat ctgcataaac tgggcgttga tgccatctgg
    10441 ctaaccccct tttatgtctc tccccaggtc gataacggtt acgatgtagc gaactatacg
    10501 gcgattgatc ccacctacgg cacgctggac gattttgacg aactggtgac gcaggcaaaa
    10561 tcgcgcggga ttcgtatcat tctcgatatg gtgtttaacc atacctctac ccaacatgcc
    10621 tggtttcgcg aggcgctgaa caaagaaagc ccttaccgcc agttttatat ctggcgcgat
    10681 ggcgaaccag aaacgccacc gaacaactgg cgttcaaaat ttggcggtag tgcgtggcgc
    10741 tggcatgcgg aaagcgaaca gtactatttg catctctttg caccagaaca ggcggatctc
    10801 aactgggaga atccagcggt acgcgcagag ctgaaaaaag tctgtgaatt ctgggccgat
    10861 cgtggggtcg acgggttgcg cctggatgtg gtgaatctga tctccaaaga cccgcgtttc
    10921 cctgaagacc tggatggcga cgggcgtcgc ttctacaccg acgggccacg agcacacgag
    10981 tttttgcacg agatgaaccg cgatgtgttt acgccacgcg ggttaatgac cgtaggtgaa
    11041 atgtcctcca ccagccttga gcattgccag cgatacgcgg cactgacagg cagtgaattg
    11101 tcgatgacct ttaattttca tcacctgaag gtcgattatc ccggtggtga aaaatggacg
    11161 ctggctaaac ctgactttgt ggcgttgaaa acattgttcc gccactggca acaaggaatg
    11221 cacaacgtgg catggaatgc cttgttctgg tgtaaccacg atcagccgcg cattgtttct
    11281 cgctttggtg atgaaggtga ataccgcgtg cctgcggcaa aaatgctggc gatggtgctg
    11341 catggcatgc agggaacgcc gtatatctac cagggcgaag agattggcat gaccaacccg
    11401 catttcacgc gcattactga ctatcgcgac gtggagagcc tcaatatgtt tgccgagctg
    11461 cgcaacgatg gtcgtgatgc cgacgagtta ttggcaatcc tcgccagtaa atcccgtgac
    11521 aacagccgca cgcccatgca atggagcaac ggcgataatg ccgggtttac ggctggcgaa
    11581 ccgtggattg gcctgggcga taactatcaa caaatcaacg tagaagccgc gctggccgat
    11641 gactcctcgg tgttttacac ctaccaaaag ttaatcgcac tgcgtaagca agaagccatc
    11701 ctgacatggg gcaattacca ggatctgctg ccaaacagcc ctgtattgtg gtgctatcgc
    11761 cgtgaatgga aggggcaaac cttgctggtc attgccaacc ttagccgtga gatccaaccc
    11821 tggcagccag ggcaaatgcg cggcaactgg cagcttgtga tgcataacta cgaagaagcc
    11881 tcaccacaac cctgtgccat gaatttacgg ccttttgagg ctgtctggtg gttacagaag
    11941 taaatcttcc ctaagccccg gtaatgccgg tcattccggg gttttgtctg tcagcatgtt
    12001 gtttttgttg atttttcaac cagcaaattc attaaaaaat ttacataacg ctgtagcgcc
    12061 cgtcatccgt acgctctgct ttttactttg agctacatca aaaaaagtta aaacatcctt
    12121 gatgcaaagc actatatata gactttaaaa tgcgtcccaa cccaatatgt tgtattaatc
    12181 gactataatt gctactacag ctccccacga aaaaggtgcg gcgttgtgga taagcggatg
    12241 gcgattgcgg aaagcaccgg aaaacgaaac gaaaaaaccg gaaaacgcct ttcccaattt
    12301 ctgtggataa cctgttctta aaaatatgga gcgatcatga caccgcatgt gatgaaacga
    12361 gacggctgca aagtgccgtt taaatcagag cgcatcaaag aagcgattct gcgtgcagct
    12421 aaagcagcgg aagtcgatga tgccgattat tgcgccactg ttgccgcggt tgtcagcgag
    12481 cagatgcagg gccgcaacca ggtggatatc aacgagatcc agaccgcagt tgaaaaccag
    12541 ctgatgtcgg gtccatacaa acaactggct cgtgcttaca tcgagtaccg tcacgatcgc
    12601 gacattgaac gtgaaaaacg cggtcgcctg aaccaggaga tccgtggtct ggtcgagcag
    12661 accaacgcct cgttactcaa cgaaaacgcc aacaaagaca gcaaggtgat tccaacccag
    12721 cgcgacctgc tggccgggat cgtggctaaa cactatgcac gccagcacct gctgccgcgt
    12781 gacgtggtgc aggcacatga gcgtggcgat attcactatc acgatctcga ttactcgccg
    12841 ttcttcccga tgttcaactg catgttgatc gacctgaaag gcatgctgac ccagggcttt
    12901 aaaatgggga acgccgagat tgaaccgccg aagtcgatct ctacggcaac cgcggtaact
    12961 gcgcagatta ttgctcaggt cgccagccat atttatggcg gcaccaccat taaccgtatc
    13021 gatgaagtgc tggcgccgtt tgttaccgcc agctacaaca aacatcgcaa gaccgcagaa
    13081 gagtggaaca tcccggacgc cgaaggctac gctaactctc gtaccatcaa agagtgctac
    13141 gatgccttcc agtcgctgga gtacgaagta aacaccctgc acaccgccaa cggtcagacg
    13201 ccgtttgtaa cctttggttt tggtctgggc accagcaggg aatcgcgcct gattcaggaa
    13261 tcgatcctgc gtaaccgtat cgcaggcctc ggtaaaaacc gtaaaactgc ggtgttcccg
    13321 aaactggtgt ttgcgattcg cgatggcctg aaccataaaa aaggcgatcc gaactacgac
    13381 atcaaacagc tggcgctgga gtgcgcaagc aagcgcatgt atccggatat cctgaactac
    13441 gatcaggtag tgaaagtcac cggttcgttt aaaaccccga tgggctgccg cagcttcctc
    13501 ggcgtgtggg aaaatgaaaa cggcgagcag atccacgatg gtcgtaacaa cctcggcgtg
    13561 atcagcctga acctgccgcg tattgctctg gaagcaaaag gcgatgaagc caccttctgg
    13621 aagctgctgg atgaacgtct ggtgctggca cgtaaggcac tgatgacccg tatcgctcgt
    13681 ctcgaaggcg tgaaagcgcg cgtcgccccg atcctctata tggaaggtgc ttgtggcgta
    13741 cgtctgaatg ctgacgatga tgtttctgaa atcttcaaaa acggtcgtgc gtctatttcg
    13801 ctgggttaca tcggcatcca cgaaaccatt aacgcgctgt tcggctgcga gcatgtttac
    13861 gacaacgagc agcttcgcgc gaaaggtatc gccattgttg aacgtctgcg tcaggcagtg
    13921 gatcagtgga aagaagaaac gggttatggt ttcagtctct acagcacgcc gagtgaaaac
    13981 ctgtgcgacc gcttctgccg tctcgatact gctgagtttg gcgtggtgcc gggcgtgacc
    14041 gataaaggtt actacaccaa cagtttccac cttgatgtgg agaagaaggt gaacccgtac
    14101 gacaagatcg actttgaagc gccttacccg ccgctggcga acggtggttt catttgctac
    14161 ggcgagtatc caaacattca gcacaacctg aaagcgctgg aagatgtctg ggattacagc
    14221 tatcagcatg taccgtatta cggcaccaat acaccgattg atgagtgcta cgagtgtggc
    14281 tttaccggtg agttcgagtg caccagcaaa ggcttcactt gcccgaaatg tggtaaccat
    14341 gacgcctccc gtgtgtcggt aactcgccgc gtgtgcggat atttaggtag cccggatgcg
    14401 cgtccgttta acgccggtaa gcaggaagaa gttaagcgcc gcgttaaaca tttggggaat
    14461 gggcagatag gttaatcttc actctgcgtt aagcaaatgc cggatgcggc gtaaacgcct
    14521 tatccggcct acatttggca acggctgtag gcctgataag acgcgacagc gtcgcatcag
    14581 gcattgatta ccggatgcag cctacacgcg gactgataag ccaggcgaaa taatgaatta
    14641 tcatcagtac tatcctgtcg acatcgtcaa cggccccggc actcgctgca ccctgtttgt
    14701 ctccgggtgt gttcatgaat gccccggttg ctataacaaa agcacatggc gggtaaattc
    14761 tggtcagcca tttaccaaag caatggaaga caagatcatt aaagatctga atgacactcg
    14821 tatcaaacgc caggggattt ccctctccgg cggcgatccg ctgcatccgc aaaacgtgcc
    14881 ggatattctg aaactggtaa aacgcatccg cgccgagtgt ccgggtaaag acatctgggt
    14941 gtggacaggc tataaactcg acgaactcaa cgctgcgcaa atgcaggttg ttgatttgat
    15001 taacgtgctg gtcgacggca aatttgtgca ggatttaaaa gacccttccc tgatctggcg
    15061 cggcagcagc aatcaggtgg tgcatcattt gcgatgataa gttccctgtt acctgagtgc
    15121 aggcaacagg gaaatgagga attaacgata cttctggtga taggcgttgc gggtcgtttt
    15181 cagttgctct gcagcagcct gcgcttcttt tactttacct tcatttgcca gcttcagcgc
    15241 gtcgtcaatc tgaccgacca gaatgtcgaa accgtggcgg aaatctttca tttccgggct
    15301 gtccggtgat ttatcttcga gcttcggcgg cgttgctttt tgcgcatcca gcgctgcggc
    15361 gcgcatcttc gttaacgcgt ctttgacttg cgtcgcgtta tcggcttttt cgaccacttt
    15421 taaattgtcg ttgagggttt ccatattgtc ttcaagatca gcggcaaacg acgccgaact
    15481 gaataccaac gaggagactg caagaatagc taacaggctt ttacgcattg ctcacttcct
    15541 tttttattta cacttcatcc ttcacactgc ctcatcgaca gctacgctca ttcgaccggg
    15601 tcacgtacta cgagtacgct ccccggctct cattcacttt ccgtctcgat gcagtgcgaa
    15661 tgattttgtg taatttttta gtttatagcg cggcaggtcg cgccagtttt tttactgtcc
    15721 ggcgattttc atctccggca acagcacaga accacactgt atattactgc gtgtttcaat
    15781 atcgttaccg acggtgacaa tattgcgcca catatctttt aaattacctg cgatggtgat
    15841 ttcgctcacc ggatactgaa tttcgccatt ctctacccag aagcccgcag caccacggga
    15901 ataatcaccg gtaatggcac tcacgccctg gcccatcagc tccgtcacca ccagcccggt
    15961 gcccatctct ttgagcattt gttcgaagct tagcccttgt ccggcaatcc gccagttgtg
    16021 aataccgccc gcatgtccgg tgcttttcag cccaagtttt cgcgccgaat agctggtcag
    16081 cagccactga gtcaggatgc catctttaat aatatcgcga cgctcggtgc gcacaccttc
    16141 gctgtcgaat ggcgtcgacg ccagcccttt cagcagatgc ggatgctctt caatggtcag
    16201 ccagtccggc agaatttgtt tacccagcga atccagcagg aaggtagatt tacgataaac
    16261 cgatccaccc gctatcgccc ccaccagatg cccaaaaagc ccagttgcca cttcattggc
    16321 aaaaatgact ggcgctttca tggtggagag tttacgcggt aacaggcgcg ataaagtacg
    16381 gcgagcacag tcggccccaa cccactctgg cgtttgcaga tcgctcatcg cgcgcccaat
    16441 ggtgtaggcg taatcacgct ccatatcgcc attttcttcg gcaattacac agctggagag
    16501 cgaatgacgc gttgagcagt aaccctgcaa catgccgtgg ctgttgccaa aaactttgac
    16561 accgtagtgg ctgttaaagc tgccaccttc ggtattggtg atacgtttat ccgcctgtaa
    16621 tgctgcctgt tctgcgcggg ccgccagttc aatggcttca tccggggaaa cttccgcagg
    16681 gtggaacaag tcgagatctg gtgcgtcaaa ggccagcagc tctttgtctg ccaggccggc
    16741 acagggatct ggcgaggtat aacgggcaat atccagcgcc gcctgtacag tacgggcaat
    16801 ggcctgcggg cttaaatcgg tggatgatgc gctacctttg cggttctggt gataaacagt
    16861 gatccccagc gcgccatcgc tattgaattc aacattctcc acttcaccat aacgcgtgct
    16921 tacgctaatg ccggtggtct tgctgacggc aacttccgca ccgtccgatt tgcctgaggc
    16981 caactccagc gcagtggaaa ctgcttcttc cagaatcttg cgctgcgctt caacttgaga
    17041 gattactttc attgcaagtg ccataatgta gagagtttct ctgaagtcta acagagaacc
    17101 gtttttcagt gcgcatctta actggtaaca ttagcctctt tttttaagga gcctgagatg
    17161 actaagcagc ccgaagactg gctcgacgac gttcccggtg atgacatcga agacgaagac
    17221 gatgaaatta tctgggtcag taaaagtgaa attaaacgtg atgccgagga gctaaaacgc
    17281 cttggcgcgg aaattgttga tctggggaaa aacgcgctgg ataagatccc gttagatgcg
    17341 gatctgcgcg cggctattga gctcgcccag cgtattaaga tggaaggtcg ccgccgccag
    17401 ttgcagctca tcggtaagat gctgcgccag cgcgacgtag agcctattcg tcaggcgctg
    17461 gataagctga aaaaccgtca caaccagcag gtggtgctgt tccataaact ggaaaacctg
    17521 cgcgatcgtc tgatcgatca aggtgatgac gccatcgctg aagtattgaa tctgtggccc
    17581 gatgccgatc gtcagcaact gcgtacactg atccgcaacg cgaagaaaga gaaagaaggg
    17641 aataagccgc cgaaatccgc acgccagatt ttccagtatc tgcgcgaact ggcggagaat
    17701 gaagggtaat tttcgttgtt gatgtgagta tggtttgccg gatgcggcgt aaacgcctta
    17761 tccggcaatg ctatatcagg ctgaagccga attactgcgc agcttccgcc ttcttcgcca
    17821 gaccatccag cagtttctga tggatcccac caaaaccgcc gttgctcatc accagaatat
    17881 ggtcgccagg ctgagcggtt ttcaccacca tatctgccag cgtatccaca tcgccactcc
    17941 agtgtgcagg ctgaacgcag gcttctgcca cttctgccac ctgccacgga atatgcgccg
    18001 gttgcagcag gaagacttca tcggcacgac ctaatgaagg tgccagatcg tctttgcaga
    18061 tccccatttt catggtattc gagcgcggtt ccagcacagc aataatgcgc gccgtaccac
    18121 caactttgcc acgcagcgcc gccagcgttg ccagaatcgc cgtcgggtga tgggcaaaat
    18181 cgtcatatac cgtgacgcca ttcgcttcac cacgcaactc cagacggcga cgagcattaa
    18241 taaacgaacc cagcgcgtta gcggcatctg ccgacgctac accaacatgg cgagccgctg
    18301 caatcgccat caggccattg tgcatattat gttcgcctac cagcgaccat ttcacttcgc
    18361 ccactttttc gccatccagc aaaacttccc attcggaagc atcggtggtc agctttttcg
    18421 cctgccagtg accctgctca cccaccagct cctgctcgct ccagcagccc atcgccatgg
    18481 tctgtttcag gttgatgtca ttttccggcc agataatacg gccctgcccc ggaacgatac
    18541 gcaccagatg gtggaactgt ttctggatcg ctttcaggtc gtcaaagata tcggcgtgat
    18601 cgaactcaag gttgttgagg atcagcgtac gcgggcagta atggacaaat ttagagcgtt
    18661 tgtcgaagaa ggcgcagtcg tactcatccg cttcgataac aaagaagtcg ctttcgccca
    18721 gacgcgccga aacctcaaag ttccccggca caccgccgat cacaaatccc ggtttgtaac
    18781 cacactgttc cagaatccag gtcgccattc ccgcggtggt ggttttgccg tgtgtaccgg
    18841 caacggccag cacccagcgg tcgcgcagca caaaatcgtg cagccactgt ggacctgaca
    18901 tataagggat gtttttttcc agtaccgctt ccacacacgg atttccacgg gtcatggcgt
    18961 tgccaataat caccagatcc ggctgcggct cgagctggct ggcatcgtaa ccctgaatca
    19021 gctcaatgcc ttgcttctca agtaaggtgc tcatcggcgg atacacattg gcgtccgaac
    19081 ccgttacttc atggcctaac tggcgcgcca gcatcgccag accgcccata aatgtgccac
    19141 aaattcctaa aatatgaatg cgcatacgtc actatccttt tttaatctgc cgctcatttt
    19201 acgcatatgt cgggcaagtg agaaacgcat ttcaggataa tccgcaattt gctggcgcga
    19261 ttcacctgag cgcaacattg agattatttg ttaagattgt tgcggtcgct ttactccata
    19321 aacattgcag ggaaagtttt atgaaaacgt taggtgaatt tattgtcgaa aagcagcacg
    19381 agttttctca tgctaccggt gagctcactg ctttgctgtc ggcaataaaa ctgggcgcca
    19441 agattatcca tcgcgatatc aacaaagcag gactggttga tatcctgggt gccagcggtg
    19501 ctgagaacgc gcagggcgag gttcagcaaa aactcgactt gttcgctaat gaaaaactga
    19561 aagccgcact gaaagcacgc gatatcgttg cggggattgc ctctgaagaa gaagatgaga
    19621 ttgtcgtctt tgaaggctgt gaatacgcaa aatacgtggt gctgatggac cccctggatg
    19681 gctcgtccaa catcgatgtt aacgtctctg tcggtaccat tttctccatc taccgccgcg
    19741 ttacgcctgt tggcacgccg gtaacggaag aagatttcct ccagcctggt aacaaacagg
    19801 ttgcggcagg ttacgtggta tacggctcct ctaccatgct ggtttacacc accggatgcg
    19861 gtgttcacgc ctttacttac gatccttcgc tgggcgtttt ctgcctgtgc caggaacgga
    19921 tgcgcttccc ggagaaaggc aaaacctact ccatcaacga aggaaactac attaagtttc
    19981 cgaacggggt gaagaagtac attaaattct gccaggaaga agataaatcc accaatcgcc
    20041 cgtatacctc gcgttatatc ggttcactgg ttgcggattt ccaccgtaac ctgctgaaag
    20101 gcggtattta tctctaccca agcaccgcca gccacccgga cggcaaactg cgtttgcttt
    20161 atgagtgcaa cccgatggca ttcctggcgg aacaagcggg cggtaaagcg agcgatggca
    20221 aagagcgtat tctggatatc atcccggaaa ccctgcacca gcgccgttcg ttctttgtcg
    20281 gcaacgacca tatggttgaa gatgtcgaac gctttatccg tgagttcccg gacgcgtaat
    20341 ctcctgcgcc tctccacacg gagaggcgtt tttattgctg cgcaatgttg actcttgtca
    20401 ctggcgaact ctgacgattc tcccacagca ccgttaatcc acgctgtaat gctataaaaa
    20461 taaacaacaa aataccgatg gcgattttcg tccaccagga actcagcgtg ccatcaaagt
    20521 ttatgtaagt ctgaatcagt ccctgaatcg ccacgccaaa aagcgtccct aataccgttc
    20581 caacgccacc gctcaaaagc gtaccgccaa ttaccactga ggcgatagca tccagctcca
    20641 cacctacgcc cgccagcgca tatccggcct gggtataaat cgagaagaca atccccgcca
    20701 gcgttgccag tccggtggag agcatataaa tgcgaatagt ggtgctgcga gtggaaatcc
    20761 ccatcagatt cgccgacgtt gcgttgccgc caatggcgta tacctgatta ccaaaacggg
    20821 tacgatgcgc gaggaatatg ccgataacca ccaccgccaa catcagcagt cccatcgcac
    20881 ttaagcgacc gccgccaggg attttccacg caaggcttga gagcgtgtca taaatgggat
    20941 ggtttatcgg aatcgactct tccgaaacga gatagctgac gccgcgcaaa aagaacatcc
    21001 ccgcaagcgt aatgatgaac gccgggatct tcagggcgtc gatcagaagc cccataaatg
    21061 cgccgaaggc acagcccatc accagcacca gcggaaacgc cagcagcggc gagaggccga
    21121 aatcgccaat cacttttgcc agaaacacgc cggtaaaggc gatcaccgaa ccgacggaga
    21181 gatcgatccc accggagagg atcacaaagg tcatgccaac ggcaatgatc ccaagaaagg
    21241 cgttatcggt caggatattg cagatcactc ttgtggaagc aaaaccagga aactgcgtca
    21301 ggcagtagag atagcccagc acaaagacgc cgatggtgat cataagcggc aagttacgtt
    21361 ttatcacggc tacgcactcc tttaatcaga ctgataaagc gttgcgactg aacaatcagc
    21421 acacaaagca ccaccaccgc tttcaccacc tggttcatct ctggcggaaa gcccgaaagc
    21481 aaaattccgg tattcattcc ctgaataatc agcgccccaa ctcccgaaag cagcaggtta
    21541 aagcgaccgc ccatcagcga tccaccgcca atcaccaccg cgagaatggc atccagctcc
    21601 agccataatc cggcgttgtt ggcatcggca ccgcgaatat ccgccgccac gataatgccc
    21661 gcaatcgccg cacatagccc gctcaacacg taggtgagca tgacgatgat tcgtgtgttg
    21721 actccggcat tttttgccgc ccgaatgttg ataccaacgg cttcgataaa catccccagc
    21781 gccgttttgc gggtcaacag ccagaacagg agaagcgtca gcaccgcgat aatgaccggc
    21841 gttggcagga acaacagcga tccgctgcca aaccatgaga gatccggcga gttaaacgtg
    21901 acgatctgcc cggcggtgat cagttgcgcc acgccgcgcc cggcgaccat caggatcagg
    21961 gtggcgacaa acggctgaat tttgaggatc gctaccagta tgccgttcca caatcccgct
    22021 aggatgccgg tgcccagggc gcttaacaaa acaatcggca ggctgaatcc cgcgaccgtc
    22081 atcgcagccg ttgtggctcc ggcgatagcc atcaccgccc ccacagagag atcgatacca
    22141 ccggtggcga tcaccagcgt cataccaatc gccagtaacg caacgggagc tgcacggtta
    22201 agaatgtcga tagggctacc gaacaaacgc ccatcctgaa gcaccacctg ccagaaatgc
    22261 ggggctacca ggctatcgac cagcagcacc agcaacagtg ccgccagctg cggcatcccc
    22321 gtcggccagt gaaaacgcct ttttggcggc gtagtgtcag ggagagattg aggcatcaca
    22381 ctgttctcct tacgccgcaa tggcgttcat gatcgccgga acggaaagct gtgccagcgg
    22441 gatctccgcc acctgtttgc gatcgcgcat gatgatcacc cggtcggcat agtccaccag
    22501 ctcttccagt tcggaggaga tcaccagcag cgccagaccg tcagcacaca gcgtttcaat
    22561 caggcggatg atctcggcgt gggcaccaac atcaatgcca cgggttggct catcaaggat
    22621 cagaaattgc ggtcgggtca gtagccaacg tgaaagcagc actttttgct gattgccacc
    22681 ggagagaaat tcaatcggtt gttcagttga aggtgtgcga atgccaagct ggcggataaa
    22741 gcgttcggca atctcttgct gctctttgcg ggaaatgggt cgtagccagc cgcgctgggc
    22801 ctggagagcg aggatgatat tttcccgcac cgaggcggcg gcgatgatgc catcggtttt
    22861 cctgtcttcc gggcagaatc cgatgcccag tactgaagcc tgatgtggcg atcgcagggt
    22921 ttgcggtttg cctttgatca acgccgtgcc gctgtcagca ggtttgatac cgaagatcac
    22981 ttcggcggtt tcggtacgtc cggatcccag caagccagcc agaccgacga tctcgccggg
    23041 gcgcacttcg agatcaaacg gtgctatcgt tccttttttg ccgtaatttt tgaacgcggc
    23101 aacgggtttg tcgctcaaca atgttcgccc ggcacgctgt agcacgtggg tatccagctc
    23161 gcgccccagc atcattttta ccagttcgat ctgcggtagc tcgcgcgttt cccgacagcc
    23221 tacgaaactg ccgttgcgta agacagtgat ccgatcgctg acctgataga cctgatcgag
    23281 aaagtgagtg acaaagatca ggctgacgcc gcgatcgcgc aactgacgca tcaggccaaa
    23341 taatagctcc acttcctggg tgtcgaggct ggcggtgggt tcatcgagga tcagcacttt
    23401 ggcggagaga tcgatagccc ggcaaatggc gacgatttgc tgcatcgcga ctgaaaagcg
    23461 gttaagcggt tcgcgcacgt cgaggaagaa accgtaagat gccatcagtt cggtggcgcg
    23521 cttttccatc tctttacggc gtagaaggcc gaaacgtttg ggttcgcgac ctatgaatag
    23581 attatcagca accgacatat tgggtagcag gttgacttcc tgatagacgg tgccaatacc
    23641 gagttgttgt gcatgggcgg tatttttcgg tgagatagcc tggccttcca gccagatggt
    23701 gccgcgatcg gcgtggtata caccagttaa tgctttgatt agcgttgatt ttcccgcccc
    23761 gttttcacca agcagcgcca tgatttcgcc acggcgcagg ctgaaatcaa cgttgtctaa
    23821 cgctttgacg ccggggaaaa atttacttaa tccttcggtg cggaggatct cctggtgttg
    23881 gtcggtcgtc atggtttccc ccggtgtaaa gcgtgctccc agtaggcatg ataagacgcg
    23941 gtaagcgtcg catcaggctt cagtgcacca tcgccggatg cggcgcgagc gccttatccg
    24001 gcctacgaat cggcatcagg catagcaacc ctcaataccc catatttttc ttcttctcta
    24061 actcttcttt tgcggtatca ggcaggtaaa gggtggattt ggttaacgtc agcttttcag
    24121 gcatggtgcc gtcttttttg tatttctcca gcgcatcgaa agcgggtccc gccatgttcg
    24181 gcgtcagttc aacactggcg ttcgcttcgc catccatcat cgctttgtag atgtccggta
    24241 caccgtcaat ggaacccgtg aggatatctt tgcccggttt caggcccgct tctttaattg
    24301 cctgaattgc accaatcacc atgtcgtcgt tatgggcgta aaccatgcag atgtttttgc
    24361 cgttgttttc cgctttgata aagctctcca tgacttcttt gcctttactg cgggtgaagt
    24421 cacctgactg cgagcggata attttgatat ttggcgcatt cttaatggct tcggcaaagc
    24481 ctttcttacg gtcaatggcg acgctggccc caacggtgcc ctgcagctcc accacgttgc
    24541 atggtttgcc attcacttct tttaccagcc agtcaccgat caacttacct tcgaggatgt
    24601 tgtcggcagt aacggtggtc atatagagag atttgtcttt cacatcgatg gaacggtcga
    24661 gcaagaagac cgggatttcg gcatctttcg cctcttttaa taccggttcc caacccgtcg
    24721 caaccactgg agcgataaag atcgcatcca ccccttgtgc aacgaaggaa cgtaccgctt
    24781 taatctggtt ttcctgcttt tgctgaccat cggcaatttt caacgtaatt ccgcgttttt
    24841 cggcctcact tttcgccaca ttggtttctg cggcgcgcca gccggattcc gatccgacct
    24901 gcgaaaatcc tccggttaat ggagctgcca acgccataga cgacatggct gccgagactg
    24961 cagagactac aagtaagcgt ttccacatag ggttgtcctc gtcggggtaa ttattattgg
    25021 tgaaaagatg ttcgcgaaaa aactatagac aattcgttat gtaacggatt gcgttacatc
    25081 acacttcaaa aaagtaaata aaacgttaat cacaagtttg taatcgcttt catctcacta
    25141 tgaaaaatgc ggctacggtt atggattttc ctgctctgta taccgtctta aaactggcga
    25201 aaaaggaaaa tgaagacgaa aacaagcaaa gacattcggc gcgagttggc tataatactc
    25261 ggcacttgtt tgccacatat ttttaaagga aacagacatg agcttactca acgtccctgc
    25321 gggtaaagat ctgccggaag acatctacgt tgttattgag atcccggcta acgcagatcc
    25381 gatcaaatac gaaatcgaca aagagagcgg cgcactgttc gttgaccgct tcatgtccac
    25441 cgcgatgttc tatccgtgca actacggtta catcaaccac accctgtctc tggacggtga
    25501 cccggttgac gtactggtcc cgactccgta cccgctgcag cctggttctg tgatccgttg
    25561 ccgtccggtt ggcgttctga aaatgaccga cgaagccggt gaagatgcga aactggttgc
    25621 ggttccgcac agcaagctga gcaaagaata cgatcacatt aaagacgtaa acgatctgcc
    25681 tgaactgctg aaagcgcaaa tcgctcactt cttcgagcac tacaaagacc tcgaaaaagg
    25741 caagtgggtg aaagttgaag gttgggaaaa cgcagaagcc gctaaagctg aaatcgttgc
    25801 ttccttcgag cgcgcaaaga ataaataagt tcttctagcg caataaccct gaacgccggg
    25861 cttcggttag taagggtttt ttatgcccgc gataaataaa ctctctattc caccatcatt
    25921 attctcagcg gttgcaaggc ttgaacggta agaacaagca aacccgacca ccattttgct
    25981 gttcatagcc acttgctgga agttagccga cctcactcat actcaccgtt caaagcgtgc
    26041 ccccgtttta cccctgcaca tttgtgaacg ccagcagagt tttttatgcg attgcagata
    26101 tggaaagcgg ctcggagaga aaagcaaaag atggaaaagt tacaaaaaaa aatcgcgaga
    26161 tacatcgcat aaaacaacgc cacccgaagg tggcgtgctt ttattattac ttatccctgt
    26221 ctaaccagtc gccgctttca attagcttta atccatcgac gggtcgttga taaacgtaca
    26281 tccatgcact cccgtacggc gtctgaatca actggcgcgc gtattcaccg cccctggtgc
    26341 gcaaggcatc aagttcggcc agcgtggcgt tgtcaatacg ataaacttca ccgtgtaccg
    26401 ttccgttccc cggaactgcg cctggatagt ggcccaggct atacaactgg tagttatcga
    26461 tactgaaatc gcccagtaac tgggcattgg tcatccagtg actgttgcct tgtttgtggc
    26521 gtaaactgcc gtagacaaat attcgcattg ctaaaactcg aactgataga gcaaatccag
    26581 tgcctggtct acaccagaca cggcttccag atatagctta ggcatcaggc gataacgtaa
    26641 cgtgagtgtt gctatagagt caaatatacc cacgccgtat ttcacttgca gacctggcaa
    26701 tacatagccg ctgaccacta cctgggagga gtcgcctact ccctgggtgt cgagcgctaa
    26761 attgcttacg ccaaacgtct cgccgatttt acccacaatc tggccacttt gcgcaacccc
    26821 cagaccaatc agcatcgagg tcattgccgc actgtcgctc tgatcgctct ccagcccttg
    26881 tccacgtagc aaataagaca atgcagcttg ttgcgacatc gccgggtcag agaagatctc
    26941 cgctttcggt tcgtccgcca gaccagtgac gcgaactccg gcaattacgt cgtcttctgt
    27001 agcatccggg ttacgaatag cttcaatatt aagatacggt tgatccggcg gaccagagaa
    27061 cagtaactca cccttacgca caatcagatc ctgaccatag gcatggaagc gcccttcagg
    27121 gatgttgatc tgcccgttca ggcccagccc ttgtttgtcc tgtacgacgt taagatcgcc
    27181 cgtcagccgc gctttcaggc caaaggcgtc aatgcgcaca ttgttgccaa cgtggacaat
    27241 caggttactg ttaatcggaa tcgacgccgt tttcggctct tccggttgca ggttatcgtt
    27301 aagcatcacc acatcgctgg agacgcctac tgcgctttcc ggcagatcgt gcaccacgat
    27361 gcgcgcccac gggacatcca cgcgaccatc gagggtaaac aggtttggtg tagcctcgaa
    27421 tacaacatct ggcgatacat ccattcgtac catcggcggc acggtgatcc gcactttact
    27481 gcctttcgcc gttactcgtg cccgccagtt ttcaatttgg ctccagtcgg catcaccgtt
    27541 caggtagatt tcaccctgct gggtccgtac tgtaccggca agcgtcgagc gcataccgtt
    27601 aaagttgacc gcaagctggc tcggctgcat atcaaacggc ataaagttgc cgtcgatatc
    27661 cacacccgta acctgaagct gaccaaacaa ctgcgggctt tgcacatcac cacccagacg
    27721 caagttggca ctcaccatcc ccgctgcttt ttccccacgg gtaaagatgg ggtttatcat
    27781 cgcaaggttg aagttacgga tattgacgtt gccaccaaga ttacggcggc cttgcggatc
    27841 ggtcacctgc acctgtccat caaactggcc gttattggtc aggcggatgg tccagcccaa
    27901 ttcggcacgg ttgttacgca attccgccgt cagattcagt gtctgaaacg ccaccggcag
    27961 cgccgcatcg ttgacggttt gcgttacctg cacgttacgc cccgaaaggg tgatactgcc
    28021 ctgcggcagc ccctctttcg tggtgtccca ggcaacatcc gctttacccg tgaagatacc
    28081 gctggcctga gtggtttctg gcataaacgg tttcagcatg gcgaggtcga agcggttgag
    28141 attcaccacc gcacgccctt cggcccccgc atcgatagtt tgcggcacgc acagttccgc
    28201 attcgggtta agccagcagt gtggcccgat gctgattttt tgctccttat tgcggtaatc
    28261 cagcgcaata tcgcgggtca gcgaccacgg gccgaccggc gtctggaaac gggtattgct
    28321 aagagttccc ttccagcgtt cttctttgcg atcaaaactt cctgccagat taagctgccc
    28381 ggagacaggc tcgccctgaa tccgcaactg tagctcgtgc tgcttttcgc tgcctttggc
    28441 attcagggtg acgaggttga tatttacatc cggctgcgaa atttgctcaa cgcgtacgtc
    28501 gagtttcccg gcgatctgat cggtggattt aatgtcgcct tccacgcgaa cctgcgccac
    28561 ggaaagttcc tgccagcgca ggccgcgcgc ggtgatatct gccagtagtt gtggcgcttc
    28621 caccgtgccg cgtactttca ccagcccttt cgctgtaccg ccaagccccg gcagcgcgtt
    28681 atccagcccc ggcgcgttga tggtggcatc aagattgaga tcttttaccc ccagctcgcc
    28741 tttcacttcg gcactgtttg gcccgagttc cagatgaagc ccagggatca tccactgcat
    28801 ataactgttg cctttcagcg tgccgtcaac gttcactttg ttctgtttga cgttaccggt
    28861 cagcttcagt tctggcacct ccatctgcca ggtgccgccg tacaggctac cgcgggtttt
    28921 aatcaagcca ttgagtttcg acggccactc cgggaactct ttggcggtgt taatgccatt
    28981 aagcgttagc tcaccgcgcc aactaatggc ctgctgccag tcgagcaacg ccttgagttc
    29041 agttttccct tccagcgccg cgacggtgag tttgtcgaga ttgacctgct gttcattacc
    29101 tttggcatca agggtaatgg tggctggcgg gatctcctgt cccttcactg ccgtacgcat
    29161 agagagcgtg taatcggtca ttttgccggt aagtttcagt ttcagatcat ccgcctgata
    29221 ctgcttctca ccagtgaacg gccagtaaag ctgtttgctg ttcacttcca cgttgagtgg
    29281 caatccggct tccgccagtc gcgtctgggc gcgtaaatcc atatccaccg gaccggaaag
    29341 gttaacgcct atctccagct gttcgcgcag cgcgccgccc actttcagct tcaccttttc
    29401 acctttcaac ggctccacgt tcagtgtgct gttgaggatg atatccaccg gccagttgtc
    29461 tgacagctgc gccgtgccgc tggcgttgac gatcccctgg ttggaatcga tatccagggc
    29521 gtccagttta gtattgccgt caatgctgct cactttcagc agcatggtgc gcacggtgat
    29581 gtccgtgtcg cccgtcacgc gcagctgctc gcccttaaac tcttcaatgt tcaggttaag
    29641 cggcaaatgc acgtcggtca tttccggcaa taccgggcga gaaaagagat ctttcagcgt
    29701 ttcgccgagc ggcttttcat ccggctgcgg attttcaatc ttcggttcga cgacctcttc
    29761 ctgcgccact tccgccactt tcggcagagc aatcagcagg cctttcagcg acgtcggttt
    29821 cagggtcagg gttttctcct gccagttcag gccggaggtg aagtccatca ccgacaccgt
    29881 ggtgtcatca atcttgatgt tgacgttatc cagcgccacc cgtgtcaggg tgatgggata
    29941 cggcgtggag agatccagcg gaccgctatc ttcctcttct tcaacctgtt cagaaggagg
    30001 cattttttta ctgtcgatgt tgacctgaat gtctttcagc gccaggtcat taatacaaac
    30061 gctactgttc cacaggcact caagaccgac cgccagatgc agattacccg cttttaccgc
    30121 cacgcctggc tgctcataac gaacgtcaga caaggtgaga tcgcgccagc cgccggtgac
    30181 gttgccaata tccagtcctg gcacccagcg atccgccgct ttaaatacaa gatgcaggcc
    30241 gctggtggtg cccaccagaa acgccaccga tcccagcaac agtaagataa cgataaccac
    30301 gccgaggctg atttttttcc ataaactcat aattctggcc ccagaccgat gtaaaactgt
    30361 aacccgtgtt cgtctttatc cgcgaccggt acggcaaaat cgagtttgat tggcccgacc
    30421 ggcgattccc agcgtacgcc gcccccggta ccggttttaa agtcgctgcg gcgaatatcg
    30481 cttaccgctt cgccactatc gacaaacacc gcgccccacc attttccggt cacgttgtac
    30541 tggtattcca gcgatccggt tatcagcttc gaggcccctt tcaggtcacc gttggcgtat
    30601 ttcggggcga tagatttata tttgtagccg cgaatactac gatcgcctcc ggcgaagaaa
    30661 cgcagatccg gcggtacttt gtcgaaatca ccggtttcaa tccagcccag cgtgccgcgt
    30721 gtcacaaaac gatggcgatc gtacagtgtg cggatccaga cgttctgcgc ctggaaaacg
    30781 gagaaatcga catctgaacc ccaggccgtg ttggagtagt cgatagagta gcgttgcgag
    30841 tcgccccagg ttggcatcag gccaccacga gaacgcgtgc ggctaatcat caccccagga
    30901 taaaacagca tcgtggtgtt ggtaatttca ccctgggtaa agtggtcgag gctccagcgc
    30961 aggttaatgg cacgctgcca gtcgctggag agatcccagt agcgagaagc caccagcgta
    31021 gtggagtcag attcggtatc gttcaggtca gtgcgcttaa aaccgccctg caccaaataa
    31081 tattgttcca gtggattctt cagcagcggc attttatagc tgaagtcgag gatctgttcc
    31141 ggcgcggaaa tactggtact ggtggtcaga ctgtgaccat aagagttcat ccacggcttt
    31201 ttccacgtcg ctttcacgcg cggtcccacg tccgtagagt aaccgacccc ggtttcgatg
    31261 gtgttttctg ttcgcggcga aaccacgccc gtcaatggta atactttcgt ttcgcgcgct
    31321 ttatcaaatt gcggtgccac taccaccgaa ttaaaccagc cggtagcaga aagtcggcgg
    31381 ttcagttccg ccagatcttt cgattcatac tcatcgccct ctttaaacgg caccagattt
    31441 tgcaggtatt catcgcggat ttgtgatcct tcaaaggtca catgcccaaa gcggtaacgt
    31501 tcgccactgt tataatcaat atcccagaag gctttatgca ggccgagcgc aatgcccagc
    31561 tgcgctttgg taaattcgct atcgaaataa cctttacgca acgcaatact ggttaaggac
    31621 tttttgaaat tttcataatc gccctggttc agtaccgtac caatagccgg gcgagtatcg
    31681 agcaatttca aatagtcttt atcggtccgc gcgccgccgc gcaataccac atcggtgccg
    31741 ccaattaaca ccggtacacc tggcgtgact ttggcgatca atacctgccg ccctttcttt
    31801 ggcggtggac ggagatcaaa ttcaatggtc ggctggtaat aacccagcgc tttcagacct
    31861 tcacggatgg catcatcgac gcgtgcgcga aagcgacggt ctggcgtcac ttcatcactt
    31921 tcaatcgtag aaagctgcgc acgaacgttc ttttccagct gtcccgataa cccctcgacc
    31981 tgtagacgga cgttcgcggc gacggcagat ccgcttaagc agagtaagct tacacagcat
    32041 aactgtcgga tatagcgcac attttctcct gaatatcctt ttttcctacc cctggaacgc
    32101 cgttaaacgg cttaacaaaa atccagtaat atggattaaa aagcagacta aaccccaaat
    32161 atttcttatg ttttacttta gacctattca cggtggttat tgtgtgcaaa tacgcctctt
    32221 gttacaacct taaccccaat gaccgatttt cgggagagcg acaccatgag tttatttgat
    32281 aaaaagcatc tggtttcccc cgccgatgcc ctgcctggac gtaacacccc gatgcccgta
    32341 gccacgctgc atgcggtcaa cggtcactca atgaccaatg tacctgacgg aatggagatt
    32401 gccatttttg cgatgggttg tttctggggt gtggagcgtc tgttctggca gttacccggc
    32461 gtttacagca ccgccgcagg ctataccggc ggctatacgc caaatccgac ttatcgggaa
    32521 gtgtgctccg gtgatacggg tcatgccgaa gcggtacgca ttgtttacga tccttccgtc
    32581 atcagctatg agcagttgct acaggtattt tgggagaatc acgatcccgc ccagggaatg
    32641 cgtcagggca atgaccacgg cacgcagtat cgttcagcga tttatccgct gaccccagaa
    32701 caggatgccg cagctcgcgc cagtctggaa cgttttcagg cggcgatgct tgccgccgat
    32761 gacgatcgtc acatcaccac ggaaatcgct aacgccacac cgttttatta tgccgaagat
    32821 gaccaccagc aatatctgca taaaaaccca tatggttact gtggaattgg cggaattggc
    32881 gtctgtctgc cgccggaagc atagcgttac gggtacaaat gtagtttgtt gataaagtgc
    32941 tctttgtcta tgcctgatgc ggcttaaacg acttaccagg cctacaaaat cgtccaaatt
    33001 caatatattg caaggactgc gtaggcctga tcggcatagc gcatcaggca gtattgcatt
    33061 tatcagcagt ctgaatttta accctctggc gactttacag taccttacgc tatactagcc
    33121 actgaaaatg tcggttcact ttcttcgaat cgactttcaa tgtgtatttc acacaaatta
    33181 atcaactttc ccttccgagg atctggcctg aaaggtcgga taagatatgt taaacagtat
    33241 tttagtcata ctctgcttga tcgctgtaag tgcgttcttc tcgatgtccg agatctcgct
    33301 tgccgcctca cgcaaaatca aacttaaact gctggctgat gaaggcaata taaatgccca
    33361 acgcgttctg aatatgcagg aaaatcccgg catgttcttt accgtggtcc aaatcggtct
    33421 gaacgcagtg gcgattctcg gcggtatcgt cggtgatgcg gcattttctc cagcttttca
    33481 cagcctgttc tcccgctata tgtcggcaga gctctctgag caactgagct ttattctctc
    33541 tttctcgtta gtgactggca tgtttatcct gtttgcggat ttaaccccga aacgcatcgg
    33601 tatgattgcg ccagaagcgg tggctttgcg tatcatcaac ccgatgcgct tctgcctgta
    33661 cgtttgcacc ccactggtgt ggttcttcaa cggcctggcg aacatgatct tccgtatttt
    33721 caaactgcca atggtacgta aagatgacat cacttctgat gacatctacg cggtagtgga
    33781 agccggtgcg ctggcgggcg tgttacgtaa acaggaacac gagctgattg aaaacgtctt
    33841 tgagctggaa tcccgtaccg ttccgtcttc aatgacaccg cgtgaaaacg tgatttggtt
    33901 tgatctccac gaagatgagc aaagcctgaa gaataaggtg gcggaacatc cgcactctaa
    33961 gttcctcgtc tgtaatgaag atattgacca catcatcggt tatgtcgatt ctaaagacct
    34021 gctgaaccgc gtgctggcta accaaagcct ggcactgaac agcggcgtac aaattcgcaa
    34081 cacgctgatt gtgccggata cgttaaccct ttcagaggcg ttggaaagtt ttaaaaccgc
    34141 aggtgaagac ttcgcggtga tcatgaacga gtacgcgctg gtggtgggga tcatcaccct
    34201 caacgacgtg atgaccacgc tgatgggcga tctggtcggt caggggctgg aagagcagat
    34261 tgtcgcccgt gatgagaact catggctgat tgacggcggc actccgattg acgacgtcat
    34321 gcgcgtgctg gatattgacg aattcccgca gtcgggcaac tacgaaacca tcggcggctt
    34381 tatgatgttt atgctgcgta agatcccgaa acgcaccgat tcggtgaaat tcgccggcta
    34441 caaatttgaa gtggtggata tcgataacta ccgcatcgac cagctgctgg tgacccggat
    34501 cgacagcaag gccaccgccc tttcgccaaa actgcctgac gctaaagata aagaagaaag
    34561 cgtcgcgtaa ccccccagaa acatcaacgg ctcctgaatc aggagccgtc ttattactgc
    34621 atagcacttt ttactgcata gcacttttta ctgcatagca ctttggttaa gccatctctg
    34681 tttgcagacg cataacctga cggttaactt cggacatcac tgacaaatgc agcttatcct
    34741 tcacttttgg gataagaatc ttacccttat caaattcaaa agcgccaacg tccttgatgt
    34801 ataaccgtcc acggaacagg atctttacgt acttcgccac ctgaagtggg ttgtaccgtt
    34861 ggaaaatttt cattgttatc tcctgctgag tattacgcct gtgcggtgcc acaatcggcc
    34921 caactattat gaggcgcaaa ttttaatgct tagtgactat agactatccg ggcaatgttt
    34981 ccaccgcgta taactttttt tacctaaagg ttacaattat tcagaattat ctttttaccg
    35041 aagcgcggtc ttcagtataa gcattcattt ttcatatgat ttgtgcgctt gaccgcaaac
    35101 tggcatcaca cttgcgggaa attcgataaa tagcacatat gattaaaact cagacccaag
    35161 tggtcggatc acctgcatat cataagaagg aaacaccatg accctacgca agattctggc
    35221 actcacctgc ctgctgttgc cgatgatggc ttccgcacat cagttcgaaa ccggtcagcg
    35281 agtgccgccg attggcatta ccgatcgggg cgagttggtg cttgataaag atcagtttag
    35341 ctacaaaacc tggaacagcg cgcagttagt gggaaaagtg cgagtactgc aacatattgc
    35401 tggtcgcacc tctgcaaaag agaaaaacgc gacgctgatt gaagcgatta aatcagcgaa
    35461 gttaccgcac gatcgttacc agaccaccac tattgttaac accgacgacg cgattccggg
    35521 ttcagggatg tttgtgcgca gcagtctgga gagtaataaa aagctttatc cctggtcgca
    35581 gtttattgtc gatagcaatg gcgtcgcgcg cggtgcctgg cagctggatg aagaaagttc
    35641 cgctgtagtg gtgctggata aggatggtcg cgtgcaatgg gccaaagacg gggcgctcac
    35701 tcaggaagag gtgcagcaag tgatggacct gctgcaaaag ttgctcaaat aatggcaaca
    35761 acatagattc tgcaaaaata attttcccct atcgttatct atgggatagg ggaattaagt
    35821 ctctgagttc ttacgccgaa ctcatactgg taacgctagt tgctcttttt taaacgcctc
    35881 aatggtcgac ttatatttct cgcaaatctt ttctttggta atattactat ctgcggccag
    35941 attcgcactt actatcgcat ccacttgttc ggctaattcc acttgttggc aacctgcatt
    36001 aaccagaaaa ttataaataa gggagtaatc ttcatcctcc aaaacaacag aggaaccatt
    36061 acgaaaacgt tcatcccctc catagatttt aagcgcaaag cccaaagatt ctatgactat
    36121 ggcaaaatta tcatcaatta ttttttcgtg aatataccat ccatcgcatt ctttaattcc
    36181 acctcctggt atttggagtt caaatttacc ccgttttaat ttgcttacta tatcctccaa
    36241 taaggcataa cctaaagaca cgtcaccaat cgcctggtga atataacctt taatcctttt
    36301 cggtatgtca gactgttttt tctgcagaat atcacaggca tacaatcttc tgttttgttc
    36361 taacggaaac tcgcggtttt tgaaatccat gaatgagtta tactcattta ttgttatggg
    36421 aatttcttcg tattcaaatc cactacctcg caatgtcatt gtatattcgg caccgttatc
    36481 tttaaggctg aaactgataa ggaacttatc catggcgtta acagttccat tttttgatga
    36541 aagtacctga taactatttt tatcaatctg agttgcgcaa cgaaagaact ccctcaaagt
    36601 taacaggcta tcataattcg aaatccaatc ttttttctta aatgcatgct ggatattgtt
    36661 aaaaaaattg tttgaatgtg tttttgtata cgatgacttt acaaataact ccaccgcacc
    36721 aataaaatta ttgtgtctga tcctggg
//
LOCUS       DAZPDU010000024        34981 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZPDU010000024 DAZPDU010000000
VERSION     DAZPDU010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34981
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.024"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..815)
                     /locus_tag="ACHURS_001067"
     CDS             complement(<1..815)
                     /locus_tag="ACHURS_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR1236859.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSS"
     gene            907..993
                     /locus_tag="ACHURS_001068"
                     /pseudo
     CDS             907..993
                     /locus_tag="ACHURS_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1003..1083)
                     /locus_tag="ACHURS_001069"
                     /pseudo
     CDS             complement(1003..1083)
                     /locus_tag="ACHURS_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1397..1873)
                     /locus_tag="ACHURS_001070"
     CDS             complement(1397..1873)
                     /locus_tag="ACHURS_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFR1236860.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1932..3221)
                     /gene="bioA"
                     /locus_tag="ACHURS_001071"
     CDS             complement(1932..3221)
                     /gene="bioA"
                     /locus_tag="ACHURS_001071"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFR1236861.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVVSAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKICDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVADIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3308..4348
                     /gene="bioB"
                     /locus_tag="ACHURS_001072"
     CDS             3308..4348
                     /gene="bioB"
                     /locus_tag="ACHURS_001072"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFR1236862.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            4345..5499
                     /gene="bioF"
                     /locus_tag="ACHURS_001073"
     CDS             4345..5499
                     /gene="bioF"
                     /locus_tag="ACHURS_001073"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFR1236863.1"
                     /translation="MSWQEKINAALDARRTADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEKFGVGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5486..6241
                     /gene="bioC"
                     /locus_tag="ACHURS_001074"
     CDS             5486..6241
                     /gene="bioC"
                     /locus_tag="ACHURS_001074"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFR1236864.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRRSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGALPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6234..6902
                     /gene="bioD"
                     /locus_tag="ACHURS_001075"
     CDS             6234..6902
                     /gene="bioD"
                     /locus_tag="ACHURS_001075"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFR1236865.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENAATGKYIN
                     LALL"
     gene            7481..9502
                     /gene="uvrB"
                     /locus_tag="ACHURS_001076"
     CDS             7481..9502
                     /gene="uvrB"
                     /locus_tag="ACHURS_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFR1236866.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9694..10602)
                     /gene="yvcK"
                     /locus_tag="ACHURS_001077"
     CDS             complement(9694..10602)
                     /gene="yvcK"
                     /locus_tag="ACHURS_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFR1236867.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10867..11011
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            10999..11988
                     /gene="moaA"
                     /locus_tag="ACHURS_001078"
     CDS             10999..11988
                     /gene="moaA"
                     /locus_tag="ACHURS_001078"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFR1236868.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEVGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            12010..12522
                     /gene="moaB"
                     /locus_tag="ACHURS_001079"
     CDS             12010..12522
                     /gene="moaB"
                     /locus_tag="ACHURS_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFR1236869.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12525..13010
                     /gene="moaC"
                     /locus_tag="ACHURS_001080"
     CDS             12525..13010
                     /gene="moaC"
                     /locus_tag="ACHURS_001080"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFR1236870.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIVGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            13003..13248
                     /gene="moaD"
                     /locus_tag="ACHURS_001081"
     CDS             13003..13248
                     /gene="moaD"
                     /locus_tag="ACHURS_001081"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFR1236871.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13250..13702
                     /gene="moaE"
                     /locus_tag="ACHURS_001082"
     CDS             13250..13702
                     /gene="moaE"
                     /locus_tag="ACHURS_001082"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFR1236872.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            13839..14543
                     /locus_tag="ACHURS_001083"
     CDS             13839..14543
                     /locus_tag="ACHURS_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFR1236873.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14814..15461
                     /locus_tag="ACHURS_001084"
     CDS             14814..15461
                     /locus_tag="ACHURS_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFR1236874.1"
                     /translation="MWLITALGLVATAGLAWGAKYLEITATKYDSPPMYVAIGLLLLC
                     MYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVPVYAIIIVFSTAGAMFLISMLAG
                     LLFNVEPGSHRFIIMMTLTGLALVIIVNAALMSERPIWVISCLMIVLWPGIISHGRNK
                     LLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFGILAAIAITLVWQRHTCFFH"
     gene            complement(15526..16237)
                     /locus_tag="ACHURS_001085"
                     /pseudo
     CDS             complement(15526..16237)
                     /locus_tag="ACHURS_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308893.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
     gene            complement(16237..17478)
                     /gene="clsB"
                     /locus_tag="ACHURS_001086"
     CDS             complement(16237..17478)
                     /gene="clsB"
                     /locus_tag="ACHURS_001086"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFR1236875.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGDAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRNFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTRWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(17475..18236)
                     /locus_tag="ACHURS_001087"
     CDS             complement(17475..18236)
                     /locus_tag="ACHURS_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFR1236876.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18369..18779
                     /locus_tag="ACHURS_001088"
     CDS             18369..18779
                     /locus_tag="ACHURS_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFR1236877.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18741..19847)
                     /locus_tag="ACHURS_001089"
     CDS             complement(18741..19847)
                     /locus_tag="ACHURS_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1236878.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(19858..21000)
                     /locus_tag="ACHURS_001090"
     CDS             complement(19858..21000)
                     /locus_tag="ACHURS_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1236879.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQIWQMQRAEDN
                     GQTFEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEA
                     LLSTEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLL
                     STLGMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPAR
                     YFVSTLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(20993..22729)
                     /locus_tag="ACHURS_001091"
     CDS             complement(20993..22729)
                     /locus_tag="ACHURS_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR1236880.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKETTPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22722..23717)
                     /gene="hlyD"
                     /locus_tag="ACHURS_001092"
     CDS             complement(22722..23717)
                     /gene="hlyD"
                     /locus_tag="ACHURS_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFR1236881.1"
                     /translation="MKKPVVIELAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERYLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            complement(23720..24391)
                     /gene="cecR"
                     /locus_tag="ACHURS_001093"
     CDS             complement(23720..24391)
                     /gene="cecR"
                     /locus_tag="ACHURS_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFR1236882.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELLLRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGSDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24620..25984
                     /gene="rhlE"
                     /locus_tag="ACHURS_001094"
     CDS             24620..25984
                     /gene="rhlE"
                     /locus_tag="ACHURS_001094"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFR1236883.1"
                     /translation="MSFDYLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            complement(26216..26698)
                     /gene="ybiA"
                     /locus_tag="ACHURS_001095"
     CDS             complement(26216..26698)
                     /gene="ybiA"
                     /locus_tag="ACHURS_001095"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HFR1236884.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26818..28968
                     /gene="dinG"
                     /locus_tag="ACHURS_001096"
     CDS             26818..28968
                     /gene="dinG"
                     /locus_tag="ACHURS_001096"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFR1236885.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRVPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTANNQEEQKRCAKLK
                     GDLDTYRWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            28996..29958
                     /gene="ybiB"
                     /locus_tag="ACHURS_001097"
     CDS             28996..29958
                     /gene="ybiB"
                     /locus_tag="ACHURS_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFR1236886.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGAALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMVCCLVAMGEAATISDGLARVNQAF"
     gene            30099..31184
                     /gene="hcxB"
                     /locus_tag="ACHURS_001098"
     CDS             30099..31184
                     /gene="hcxB"
                     /locus_tag="ACHURS_001098"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFR1236887.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            31251..31310
                     /gene="ybiE"
                     /locus_tag="ACHURS_001099"
     CDS             31251..31310
                     /gene="ybiE"
                     /locus_tag="ACHURS_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFR1236888.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(31413..31673)
                     /gene="ybiJ"
                     /locus_tag="ACHURS_001100"
     CDS             complement(31413..31673)
                     /gene="ybiJ"
                     /locus_tag="ACHURS_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFR1236889.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPITASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31938..32204)
                     /gene="ybiI"
                     /locus_tag="ACHURS_001101"
     CDS             complement(31938..32204)
                     /gene="ybiI"
                     /locus_tag="ACHURS_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFR1236890.1"
                     /translation="MASGWANDDAVNEQINSTIENAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(32278..32955)
                     /gene="ybiX"
                     /locus_tag="ACHURS_001102"
     CDS             complement(32278..32955)
                     /gene="ybiX"
                     /locus_tag="ACHURS_001102"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFR1236891.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSQHCV
                     PPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(32997..34981)
                     /locus_tag="ACHURS_001103"
                     /pseudo
     CDS             complement(32997..34981)
                     /locus_tag="ACHURS_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8269 a         8976 c         9491 g         8245 t
ORIGIN      
        1 gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata
       61 ttaacatctg cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg
      121 ttagggatat atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt
      181 tccccaacgt ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta
      241 tcaaaagcac ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg
      301 ttgccaacag aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg
      361 agaagggctg agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg
      421 ttaagtgaaa catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg
      481 ttatcgctgg ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca
      541 ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg
      601 agtaaactta aattgagggc tggctcttga aaagccagac atattttcag taatcgaact
      661 gcctgtgttc ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga
      721 taatccgtga tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag
      781 gagcggaaaa aagaaggatt gtgggcaggt ttcatttatt attgatcctc attgtttata
      841 tattaatatg ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt
      901 taagaacagg aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg
      961 ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca
     1021 gtagtctgcc caccactgca tcatttctct gcgcttatcg agatactgag catggttgta
     1081 aattcagcta acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg
     1141 cgctaatgct ctgtatcagg tcactaatac catctaagtg gttgattcat agtgactgga
     1201 tatgttgtgt tttgcagtat tatgcagtct attttttaag ctaaatgtat tataatgcat
     1261 tgatattaat gatttttact gtttctcgtt cagcttttta tactaacttg agcgaaacgg
     1321 gaaggtaaaa agacaaaaag ttgtttttaa tacctttaag tgataccaga tggcattgcg
     1381 ccatctggca gagtgattaa ctaaacatcg cagtaatcga ggcgcttgcc agagagtgga
     1441 aatgaacgtt aaacccgacc atcgcgccgc tggcaccttc atcgacatca atacgttcta
     1501 tatccagcgc gtgaacggta aaaatgtagc gatgagtttc gcctttcggc ggtgctgcgc
     1561 catcgtaccc ggttttacca aagtcggtac gcgtctgcaa aacgccgtct ggcattgcta
     1621 ccagaccaga gccaaaccct tgcggtaata cgcgggtatc agcgggtaag ttaacaacta
     1681 cccagtgcca ccagccggag ccggttggcg catccgggtc gtagcaggtg acaacaaaac
     1741 ttttcgttcc cgcaggaaca tcatcccacg ccagatgcgg tgaaatatta tcgccatcgt
     1801 aacccatgcc gttaaagaca tgacgatgcg gcaatttatc gccatcgcgc agatcgttac
     1861 tgatgagttt catgaaccct cctttcttgt ttgcagaaag tgtagccaga aaccctcacg
     1921 cggacttctc gttattggca aaaaaatgtt tcatcctgta ccgcgcggtt aaccgctgcg
     1981 gtcagacgct gcaactgttg cgggagaata atatagggcg gcatcaggta aatcagtttg
     2041 ccaaaaggcc ggatccagac accctgttcg acaaagaatt tttgcagcgc cgccatattc
     2101 accggatgag tggtttcgac cacgccaatg gcccccagta cgcgcacatc ggcaaccatt
     2161 tcggcatcac gggcgggggc aagttgctcg cgcagctgta cttcaatatc cgccacctgt
     2221 tgctgccagt cgccagattc gagaatcgcc aggctggcgt ttgctgccgc gcaggccagc
     2281 ggattgccca taaaagttgg cccatgcata aagcaaccgg cttcaccgtt actgatggtt
     2341 tctgcaacct cgcgcgtggt gagtgtggcg gaaagggtca ttgtgccgcc ggttaaggct
     2401 ttaccgaggc acaaaatgtc cggcgcgatt tctgcatgtt cacaggcaaa cagtttcccg
     2461 gtacgaccaa atccagtggc gatctcgtcg gcaatcagca agataccttc gcgatcgcat
     2521 attttgcgga ttcgttttaa ccattccgga tggtacatgc gcatcccgcc tgcgccctgg
     2581 acaatcggct caatgatcac cgccgcgatt tcatgacgat gcgccgccat caggcgggca
     2641 aagcccacca tatcgcgctc atcccattcg ccatccatac ggctttgtgg ggcgggagca
     2701 aacaagtttt ctggcagata gcctttccac aggctgtgca ttgagttgtc cggatcgcac
     2761 accgacatcg cgccaaaggt atcgccatga taaccattgc ggaaggtcag aaaacgctgg
     2821 cgcgcttcgc ctttggcttg ccagtactgc aacgccattt tcatcgccac ttccaccgct
     2881 acggaaccgg agtccgcgag aaaaacgcac tccagcggtt gcggcgtcat cgccaccagt
     2941 ttgcggcaca gctcaatggc tggcgcatgg gtgataccgc caaacatcac atgcgacatg
     3001 gcatcaattt gcgacttcat cgccgcatta agctgcgggt gattgtagcc gtggatcgcc
     3061 gcccaccagg acgacatacc gtcaaccagg cgtctgccgt cagacaaaat cagctcgcaa
     3121 ccttcggcgc tcaccaccgg ataaaccggc agcggggagg tcatggatgt gtatgggtgc
     3181 cagatatggc gttggtcaaa ggcaagatcg tccgttgtca taatcgactt gtaaaccaaa
     3241 ttgaaaagat ttaggtttac aagtctacac cgaattaaca acaaaaaaca cgttttggag
     3301 aagccccatg gctcaccgcc cacgctggac cttgtcgcaa gtcacagaat tatttgaaaa
     3361 accgttgctg gatctgctgt ttgaagcgca gcaggtccat cgccagcatt tcgatcctcg
     3421 tcaggtccag gtcagcacgt tgctgtcgat taagaccgga gcttgtccgg aagattgcaa
     3481 atactgcccg caaagctcac gctacaaaac cgggctggaa gccgagcggt tgatggaagt
     3541 tgaacaggtg ctggagtcgg cgcgcaaagc gaaagcggca ggatcgacgc gcttctgtat
     3601 gggcgcggcg tggaagaatc cccacgaacg cgatatgccg tacctggaac aaatggtgca
     3661 gggggtaaaa gcgatggggc tggaggcgtg tatgacgctg ggcacgttga gtgaatctca
     3721 ggcgcagcgc ctcgcgaacg ccgggctgga ttattacaac cacaacctgg acacctcgcc
     3781 ggagttttac ggcaatatca tcaccacccg cacttatcag gaacgcctcg atacgctgga
     3841 aaaagtgcgc gatgccggga tcaaagtctg ttctggcggc attgttggct taggcgaaac
     3901 ggtaaaagat cgcgccggat tattgctgca actggcaaac ctgccgacgc cgccggaaag
     3961 cgtaccaatc aacatgctgg tgaaggtgaa aggcacgcca ctggcggata acgatgatgt
     4021 cgatgccttt gattttattc gcactattgc ggtagcgcgg atcatgatgc cgacctctta
     4081 cgtgcgcctt tctgccggac gcgagcagat gaatgaacag actcaggcga tgtgctttat
     4141 ggctggcgca aactcgattt tctacggttg caaactgctg accacgccga atccggaaga
     4201 agataaagac ctgcaactgt tccgcaaact ggggctaaat ccgcagcaaa ctgccgtgct
     4261 ggcaggggat aacgaacaac agcaacgtct tgaacaggcg ctgatgaccc cggacaccga
     4321 cgaatattac aacgcggcag cattatgagc tggcaggaga aaatcaacgc ggcgctcgat
     4381 gcacggcgta ctgccgatgc cctgcgtcgc cgttatccgg tggcgcaagg agccggacgc
     4441 tggctggtgg cggatgatcg ccagtatctg aacttttcca gtaacgatta tttaggttta
     4501 agccatcatc cgcaaattat ccgcgcctgg cagcagggcg cagaaaagtt tggtgtaggt
     4561 agcggcggtt ccgggcatgt cagcggttat agcgtggtgc atcaggcgct ggaagaagaa
     4621 ctggccgagt ggcttggcta ttcgcgggcg ctgctgttta tctctggttt tgccgcgaat
     4681 caggcagtca ttgccgcgat gatggcgaaa gaggaccgta ttgttgccga ccggcttagc
     4741 catgcctcct tgctggaggc tgccagttta agcccgtcgc agcttcgccg ttttactcat
     4801 aacgatgtcg ctcatctggc gcgactgctt gcttccccct gtccggggca gcaactggtg
     4861 gtgacagaag gcgtgttcag catggacggc gatagcgcgc cactggcgga aatccagcag
     4921 gtaacgcaac agcacaatgg ctggttgatg gtcgatgatg cccacggcac gggcgttatc
     4981 ggggagcagg ggcgcggcag ctgctggctg caaaaggtaa aaccagaatt gctggtggtg
     5041 acttttggca aaggatttgg cgtcagtggg gctgcggtgc tttgctccag tacggtggcg
     5101 gattatctgc tgcaattcgc ccgccacctt atctacagca ccagtatgcc gcccgctcag
     5161 gcgcaggcat tacgtgcgtc gctggcggtc attcgccgtg atgaggggga tgcacggcgc
     5221 gaaaaactgg tgtcactcat tgcgcgtttt cgtgccggag tacaggattt gccgtttacg
     5281 cttgctgatt catgcagcgc catccagcca ttgatcgtcg gtgataacag ccgtgcgtta
     5341 caactggcag aaaaactgcg ccagcaaggc tgctgggtca cggcgattcg cccgccaacc
     5401 gtacccgctg gtactgcgcg actgcgctta acgctaaccg cagcgcatga aatgcaggat
     5461 atcgaccgtc tgctggaggt gctgcatggc aacggttaat aaacaagcca ttgcagcggc
     5521 atttggtcgg gcagccgcgc actatgagca acacgcagag ttacagcgcc ggagtgctga
     5581 cgtcttactg gcaatgctcc cacagcgtaa atacacccac gtactggacg cggggtgtgg
     5641 gccaggctgg atgagccgcc actggcgaga gcgtcacgca caggtgacgg cgttagatct
     5701 ctcaccgcca atgcttgtgc aagcccgcca gaaagatgct gcagaccatt atctggcggg
     5761 agatatcgaa tccctgccgt tagcgactgc gacgttcgat cttgcatgga gcaatctcgc
     5821 ggtgcagtgg tgcggtaatt tatccacggc actccgcgag ctgtatcggg tggtgcgccc
     5881 cggaggcgtg gtggcgttta ccacgctggt gcagggagcg ttacccgaac tgcatcaggc
     5941 gtggcaggcg gtggacgagc gtccgcatgc taatcgcttt ttaccgccag atgaaatcga
     6001 acagtcgctg aacggcgtgc attatcaaca tcatattcag cccatcacac tatggtttga
     6061 tgatgcgctc agtgccatgc gttcgctgaa aggcatcggt gctacgcatc ttcatgaagg
     6121 gcgcgacccg cgaatattaa cgcgttcgca attgcagcga ttgcaactgg cctggccgca
     6181 acaacagggg cgatatcctc tgacgtatca tctttttttg ggagtgattg ctcgtgagta
     6241 aacgttattt tgtcaccgga acggataccg aagtggggaa aaccgtcgcc agttgtgcac
     6301 ttttacaagc cgcgaaggca gtaggttatc ggacggcagg ttataaaccg gtcgcctctg
     6361 gcagcgaaaa gaccccggaa ggcttacgta atagtgacgc gctggcgtta cagcacaaca
     6421 gcagcctgca gctggattac gcaacagtaa atccttacac cttcgcagaa cccacttcgc
     6481 cgcacatcat cagcgcgcaa gagggcaggc cgatagaatc attggtaatg agcgccggat
     6541 tacgcgcgct tgaacaacag gctgactggg tgttagtgga aggtgctggc ggctggttta
     6601 cgccgctttc tgacactttc acttttgcag attgggtaac acaggaacaa ctgccggtga
     6661 tactggtagt tggtgtgaaa ctcggctgta ttaatcacgc gatgttgact gcgcaggcaa
     6721 tacaacacgc cggactgact ctggcgggtt gggtggcgaa cgatgttacg cctccgggaa
     6781 aacgtcacgc tgaatatatg accacgctca cccgcatgat tcccgcgccg ctgctgggag
     6841 agatcccctg gcttgcagaa aatgcggcaa ccggaaagta cataaacctt gccttgttgt
     6901 agccattctg tatttggtta aattgcgagc gagatcgcgt cttcgattga ctgcaattta
     6961 accaattaaa ttctaaaata atcacgaaaa aaattttact tccgcctcat gcggcgaatg
     7021 tgggaattgc ccaggcggcg ggggataggg gctggagaca gttatccact attcctgtgg
     7081 ataaccatgt gtattagagt tagaaaacac gaggcaagcg agagaatacg cggcttgcac
     7141 gcgaattggc gttaaagacg gctcaaagaa atatctttta ttttttaact ggttagataa
     7201 atgcaatggc agtcactgaa caggcatctc ttgccataaa actgtcatca ctcatcttga
     7261 caaatgttaa aaaagccgtt gctttgggga taacccggta aggccggagt tttatctcgc
     7321 cacagagtaa attttgctca tgattgacag cggagtttac gctgtatcag aaatattatg
     7381 gtgatgaact gtttttttat ccagtataat ttgttggcat aattaagtac gacgagtaaa
     7441 attacatacc tgcccgccca actccttcag gtagcgactc atgagtaaac cgttcaaact
     7501 gaattccgct tttaaacctt ctggcgatca gccagaggcg attcgacgtc tcgaagaggg
     7561 gctggaagat ggcctggcgc accagacgtt acttggcgtg actggctcag ggaaaacctt
     7621 caccattgcc aatgtcattg ctgaccttca gcgcccaacc atggtacttg cgcccaacaa
     7681 aacgctggcg gcccagctgt atggcgaaat gaaagagttc ttcccggaaa acgcggtgga
     7741 gtatttcgtc tcctactacg actactatca gccggaagcc tatgtaccga gttccgacac
     7801 tttcattgag aaagatgcct cggttaacga acatattgag cagatgcgtt tatccgccac
     7861 caaagcgatg ctggagcggc gtgatgtggt tgtagtggcc tctgtttccg cgatttatgg
     7921 tctgggcgat cctgatttat atctcaagat gatgctccat ctcacggtcg gtatgattat
     7981 cgatcagcgc gcgattctgc gccgactggc ggagctgcaa tacgctcgta atgatcaagc
     8041 attccagcgt ggtactttcc gcgttcgtgg cgaggtgata gatatcttcc cggcagaatc
     8101 ggatgacatt gcacttcgcg tggaactgtt tgacgaggaa gtggaacgat tgtcgttatt
     8161 tgacccgctg accgggcaga ttgtttccac tattccacgt tttaccatct acccgaaaac
     8221 gcactacgtc acaccgcgcg agcgcatcgt acaggcgatg gaggagatca aagaagaact
     8281 ggccgccaga cgcaaagtgc tgttggaaaa caacaaactg ctggaagagc agcggctgac
     8341 ccagcgtacc cagtttgatc tggagatgat gaacgagctg ggctactgtt cggggattga
     8401 aaactactcg cgcttcctct ccggtcgtgg accgggtgag ccaccgccga cgctgtttga
     8461 ttacctgcct gccgatgggc tgctggtggt cgatgaatct cacgtcacca ttccacaaat
     8521 tggcggcatg tatcgcggtg accgggcgcg taaagagaca ctggtggagt acggcttccg
     8581 cctgccatca gcgctggata accgtccgct taagtttgaa gagttcgaag cattagcgcc
     8641 gcaaaccatc tatgtttcgg cgacgccggg taattacgag ctggaaaaat ccggcggcga
     8701 tgtggtggat caggtggtgc gtccaaccgg attgcttgac ccgattatcg aagtgcggcc
     8761 ggtggcgaca caggttgatg atcttctttc ggagattcgt cagcgagcgg caattaacga
     8821 acgcgtactg gtcaccacac tgaccaagcg gatggcggaa gatcttaccg aatatctcga
     8881 agaacatggc gagcgcgtgc gttatcttca ttcagatatc gacaccgtcg aacgtatgga
     8941 gattatccgc gacttgcgtc tgggtgagtt cgacgtgctg gtagggatca acttactgcg
     9001 cgaaggtctg gatatgccgg aagtgtcgct ggtggcgatc ctcgacgctg acaaagaagg
     9061 cttcctgcgt tccgaacgtt cgttgatcca gactattggt cgtgcggcac gtaacgttaa
     9121 cggtaaagcg attctctacg gcgataagat caccccatca atggcgaaag cgattggcga
     9181 aaccgaacgt cgccgtgaga aacagcagaa gtacaacgag gaacacggaa ttacgccgca
     9241 aggcttgaac aagaaagtgg tcgatatcct ggcgctgggg cagaacattg ccaaaaccaa
     9301 agcgaagggc agaggaaaat cgcgcccgat tgttgagccg gataatgtgc cgatggatat
     9361 gtcgcctaaa gcgttgcaac agaaaatcca tgagctggaa gggttaatga tgcaacacgc
     9421 gcagaatctg gagttcgaag aagcggcaca aattcgtgac cagttgcatc agctgcgtga
     9481 gctgtttatc gcggcttcgt gacaggataa cgaagaagac agatgacaaa agcaaaattg
     9541 cctgatgtgc tacgcttatc aggcctacat gatctctgca atacattgaa ttagcgcggt
     9601 ttgtaggccg gataaggcgt tcacgccgca tccggcataa acaacgcgca ctttgtcttc
     9661 aataggaagc cggaattttt cttcggattt ccgttaacct aaagcctgta acgccttttc
     9721 cagcgcgtta tgtaacaact ggcggtcatg acgatacgga atatcgctgg cctccagtac
     9781 ctcctgaatc acaatccgct ctttcaccgc cgagacatcc acttttggcc cgacgatgac
     9841 cgcatcaatg acctttttac caacatactg ctccataatt gccagcttgc tttccagctt
     9901 caaattagcc gcaggtaaac tcaactcacg ccccagattg ccgatataga ccatcggcgc
     9961 tggcgtgcgg cgtaatgcct gggcgatttc cttcagcagc agaattggca tcaggctggt
    10021 ataaaaactg ccaggcccaa taatgatgag atccgcttca ttgatagcgt gaaccgcctc
    10081 acgcgttgcg ggtacattcg gcgttaataa taactcttga atcggcgtag ttaactggtc
    10141 gatattgacc tcgccgtaaa cttcatgccc ctgatcgtca atcgccatca gatcgacggg
    10201 atgctctgac attggaatca aatgcgtatc cactttcagc agattacgaa ttaaattgat
    10261 ggcttccaga ggccgcacgc taaggtgatc cagcgccttt aacatcaagt ttccgagatt
    10321 atgaccggaa agttcgccat tgccgccaaa acggtattca aacatcgcgg aggcgacgct
    10381 cggttccgtt atcagttggt tgaggcagtt gcgcatatcg ccccaggcaa tgccgccttc
    10441 tgaacggcga atacgccccg tcgagccacc attatccgtg gtggtgacga tacccgttaa
    10501 acgagaaccc aaagacgaaa gtgatgagag aacgcgtccc agtccatgcc ctccgccgag
    10561 agcaacgaca cgatcaagat cagccagcgt acgattgcgc atatagtttc ctggagtcag
    10621 attatccgcg ctacagtagc gcaaattgcg gggaaacagc aattaacctg ccaatattga
    10681 ggatacaaac tattttcttc tacctctaaa ggacgatgca cgctatgcct ccctgatgat
    10741 gtatatcaaa gtaaaactgc cattttccct tattctgtag cgaaatagca cgatcatgac
    10801 gctatataca tgattacata gcgaaagtgt ggatggtaaa aatctcattt acacgctagt
    10861 atcggcttaa ccactaaaca ctctagcctc tgcacctggg tcaactgata cggtgctttg
    10921 gccgtgacaa tgctcgtaaa gattgccacc agggcgaagg aagaaatgac ttcgcctccc
    10981 gtatctggaa aggtgtacat ggcttcacaa ctgactgatg catttgcgcg taagttttac
    11041 tacttgcgcc tgtcgattac cgatgtgtgt aactttcgtt gcacctactg cctgccggat
    11101 ggctacaaac cgagcggcgt caccaataaa ggctttctta ccgtcgatga aattcgccgg
    11161 gttacgcgcg ccttcgccag tctgggcacc gaaaaagtgc gcctgacagg aggagagccg
    11221 tctttacgcc gcgactttac cgatatcatc gccgctgtgc gggaaaacga cgctatccgc
    11281 cagattgcgg tcaccaccaa tggttaccgt ctggaacgcg atgtggcgaa ctggcgcgat
    11341 gcgggactta ctggcattaa cgtcagtgtc gacagtctgg acgcccgcca gtttcacgct
    11401 attaccgggc aggataaatt caaccaggtc atggcaggga tcgatgctgc atttgaggtc
    11461 ggttttgaga aggtcaaagt caataccgtg ctgatgcgtg atgttaatca tcaccagctc
    11521 gacacctttc tgaactggat ccagcatcgc cctatccagc tgcgtttcat cgaactgatg
    11581 gaaacgggcg agggcagtga gctcttccgt aagcatcaca tctctggtca ggttctgcgt
    11641 gacgagctac tgcgtcgcgg ctggatccac caattacgtc aacgcagcga cggtcccgcg
    11701 caagtctttt gccatccaga ttacgccgga gagattggcc ttatcatgcc gtatgaaaaa
    11761 gacttctgcg ccacttgcaa ccgcctgcgc gtttcctcca ttggtaaact ccatctctgc
    11821 ctgtttggtg aaggcggcgt taacctgcgc gatctgctgg aagacgatac ccagcaacag
    11881 gcgctggaag cgcgtatttc agcggcgctg cgggagaaga aacagaccca tttcctgcat
    11941 caaaacaaca ccggtattac gcaaaactta tcgtacattg gcggctaaaa cgtcaaaagg
    12001 agagatcaga tgagtcaggt aagcactgaa tttatcccga cccgtattgc tattcttacg
    12061 gtttctaatc gtcgcggtga agaagacgat acctccggtc actatttgcg cgattcggcg
    12121 caagaagcgg gccatcacgt tgtcgataaa gccattgtga aagaaaaccg ctacgctatt
    12181 cgcgctcagg tatctgcgtg gatcgccagc gacgatgtac aagtggtgtt gattacgggg
    12241 gggactggcc tgacggaagg tgatcaggct cccgaagcat tgctgccgtt gttcgaccgt
    12301 gaagttgaag gttttggtga agtgttccgg atgttgtcgt ttgaagagat tggcacttcc
    12361 acgttgcaat ctcgtgcggt agcgggcgtc gccaacaaaa cgctgatttt cgccatgccg
    12421 ggttcgacca aagcgtgccg taccgcatgg gaaaatatca tcgcgccgca gctggatgcc
    12481 cgtacgcgtc cgtgtaattt ccatccacat ttgaagaaat aagtatgtcg caactgaccc
    12541 atatcaacgc cgctggcgaa gcgcacatgg tggatgtctc cgccaaagcg gaaaccgtgc
    12601 gtgaagcgcg ggcggaagcc tttgtcacca tgcgcagcga gacgctggcg atgattattg
    12661 atggtcgcca ccacaaaggc gacgtatttg ccactgcgcg tattgtcggt attcaggcgg
    12721 caaaacgcac ctgggatctg atcccgctgt gtcacccgct gatgctcagc aaagttgaag
    12781 tcaatttaca ggccgagccg gagcacaatc gggtgcgtat agaaacctta tgccgcctga
    12841 ccgggaaaac cggtgtcgaa atggaagcgt taaccgcggc ctccgtggcg gcgctgacca
    12901 tttatgacat gtgcaaagcg gtgcaaaaag atatggtgat tggtccggta cgtttgctgg
    12961 cgaagagcgg cggcaagtcg ggtgacttta aggtggaagc ggatgattaa agttcttttt
    13021 ttcgcccagg tgcgcgagtt ggtgggaaca gatgcaaccg aagtggctgc ggattaccca
    13081 accgttgaag cgttacgcca gcacctggct gcgcagagcg atcgctgggc gctggcgctg
    13141 gaagatggca aattgctggc tgccgtcaac cagacgctgg tgagttttga ccatccgctg
    13201 actgacggcg acgaagtagc tttcttcccg ccggtaaccg gaggttaaga tggcagaaac
    13261 caaaattgtt gtcggcccgc agccgttcag cgtaggagaa gagtacccgt ggctggctga
    13321 gcgtgacgaa gacggtgcag tggtcacgtt taccggtaag gtgcgcaacc ataacctggg
    13381 tgacagcgtc aaagcattaa ccctcgaaca ctatccgggg atgactgaaa aagcactggc
    13441 agaaatcgtt gatgaagcgc gtaaccgctg gccgctgggg cgcgtcacgg tgattcaccg
    13501 catcggggaa ttatggccgg gcgatgaaat cgtttttgtc ggtgtcacca gtgcgcatcg
    13561 cagcagtgcg tttgaagccg ggcagtttat tatggattat ctcaaaaccc gcgcaccgtt
    13621 ctggaagcgc gaagccacgc cggaaggcga ccgctgggtt gaagcccggg agagcgatca
    13681 gcaggcggca aaacgctggt agttttttgt tagccggata aggcaccgct gcgtccggca
    13741 atttcaccgc tcagtacatg cgtttacgct gcgtcgatgc acagcctcat cattttgcag
    13801 tatccttaag atattcctta tatcttcagg agatcgtcat ggacagattc ccacgttctg
    13861 attcaatcgt acaaccccgg gctggcttgc aaacttatat ggcgcaagtc tatggctgga
    13921 tgaccgttgg cttgttgctg accgcatttg ttgcctggta tgcggctaat tccgcggccg
    13981 tgatggagct gttgttcact aaccgtgtct ttttaatcgg tctgatcatc gcgcaattag
    14041 cattggttat tgtgttatca gcgatgattc aaaagctgag cgcaggtgta acgacgatgc
    14101 tctttatgct ttattcggcg ctgacgggtc ttacgctttc cagtatattc attgtctata
    14161 ccgctgcttc tatcgccagt actttcgtcg ttactgccgg gatgttcggc gcaatgagcc
    14221 tgtacggtta caccacgaag cgcgatttaa gtggctttgg caatatgctg tttatggcgt
    14281 taatcggcat tgtgctggca tcgctggtca acttctggct gaaaagcgaa gcattgatgt
    14341 gggcagttac ctacatcggc gtgattgtct ttgtcggatt gacggcgtat gacactcaga
    14401 aattgaaaaa tatgggtgag cagattgata cccgcgacac gtcgaacctg cgcaaatatt
    14461 ccattcttgg cgcgttaacc ttgtatctgg acttcatcaa cctgttcctg atgttgttac
    14521 ggatcttcgg caaccgccgt taattctttt tcaccatcac tatcccggcc atgtgtcggg
    14581 atttttttct tactaaaaac gacacttaac aagtaccagg tataaaatat gccttatgag
    14641 cgagcggaat cgttttccgt atccataaat acaaaagtat tttgtatgcg tggttttaaa
    14701 tttattgatg gtgaatatta atattttttc taaggacaac taaaagaatg gaatcatact
    14761 cgcaaaacag caataaatta gattttcagc acgaggccag gatataaaac ggtatatggc
    14821 tcattaccgc tttaggtttg gtggcaaccg caggactagc ctggggagct aagtatctcg
    14881 aaattacggc aaccaaatat gattcaccac caatgtatgt cgctataggg ttattattac
    14941 tttgtatgta tggcttaagt aaggatatca acaagataaa tgccgccatc gcgggcgtaa
    15001 tatatctgtt tttactctct ttggtggcga ttgtcgttgc aagtttagtt cctgtatatg
    15061 ccattatcat cgtgttcagc actgcgggcg cgatgttttt aatcagtatg ctggccggtt
    15121 tattatttaa tgttgagcct ggttctcacc gttttatcat tatgatgacg ttgacagggt
    15181 tggccctggt aatcatcgtg aatgcggcat taatgagtga acggcccatt tgggtaataa
    15241 gttgcttaat gattgtgtta tggccaggca ttatctcgca tggacgaaat aagctccttg
    15301 aactggcagg aaaatgccat agtgaagagt tgtggagtcc ggttcgttgc gcttttacag
    15361 gtgcattaac actctattac tattttatcg gcttctttgg gatacttgcc gcgatagcta
    15421 taacgcttgt ctggcaaagg catacgtgtt tttttcatta gccgtaaatg cacatactaa
    15481 agaacttaac taaacttcac atcgccgctt catttttcgc ccgtagccca gtacaaagaa
    15541 atagttcacg ctttgaccaa gtaacagcca gataatcgcc cccattacca tccagttaac
    15601 gctggaaatc agcatctgag cgagggcgaa tttccatgaa ggcagcacca gcttttgccc
    15661 tttgatggtc atatggcggt gcttcgcgaa agcgcaaaac cacaaataaa ccgcgataat
    15721 catcagtaag ccaatgccga gaatacgcag cgtagtttga tcgacatacc agtgatccgg
    15781 caactccacc acgcctgcgg taaagataat ccctgccagt aaaatatagc ccagccagtt
    15841 ggtggtaata ctgagcgaga aaatccgcgt aatagtgcta ccgggcaacc ccagacgaga
    15901 gtacaaacga tagcgcatac caatgccgcc gacccaggta ctgagcgtca ggttgaaggc
    15961 gtagcagata aacgacacca gcatcacctg gcgcttcgcc agtttgtgac cgcagtaaaa
    16021 gcgggcgagc aggtcatagc agccgtaaat cagatagctg acgaccacca gcgaccgcac
    16081 taagcagcgc aacgcgattg tagtcgcgga tgaccttcca gacctcttcc cagtccactt
    16141 ttttggcgta gaccaccagc aacacaatca ccgcgataaa aaacagccag gtgaggatct
    16201 tctttgctaa gcgccagcgc gggtgtgatt tactcatcag ggttttaccc ccgtgttttc
    16261 agtttctacc cgatcctgcg tttccattgt cggttgtgcg ggcggatcaa cctgcgccag
    16321 acgtggcgtg tgtgccggaa gccagccaac cagcgccggg aagtggcgta aaaagtggaa
    16381 cgccagcacg cttttggtca ggttccacct ggtgcgtttg ggcagcatgg tttcatccac
    16441 ctgctgacaa tctgcggcga taatgccgtt cagattatcg cgcagcgtct ggttaaaatt
    16501 acgatcgtgg atgatgacat ttgcttcgag attcagtgac aaactgagcg gatcgagatt
    16561 actggaccct actgtcgccc agtgatcgtc catcaatgct actttgccgt ggagcgggcg
    16621 gcggcggtac tcaaacacct gaacgccgcc tttaaccaga tagttataca gcaagcgcgc
    16681 gccgactctg acaatcggca tatccggttc gccctgaatg atcagtttga tccgcacccc
    16741 gcggcgtgcc gctttacgca aggcgtgtaa aaatcgatag ccggggaaga agtaggcgtt
    16801 ggcgataatg acttcccgcc gcgcctgagt gagcattttc agatagtggc gttctatatc
    16861 atcgcggtgt tcttcgttat cgcgccagac cagcaatacc tgcgcgtctc ccggctggcg
    16921 gttctcttcc gctttgtgat gacgtcgcca ccagcgtcgt gccgcgctct gtccaggcag
    16981 gttttccagc tcaaactgga gaatatcttc gacaatcggc ccttcaaggc gtaccgcgta
    17041 atcctgttta gcctctggac cgtagctgga catatgctcg gcggagtaat tcagcccgcc
    17101 aataaaggcg atacgcgcgt cgatcaccac aattttgcga tgcatccggc gaaacacatt
    17161 ggtgcgcata ccaaaaaggc gagggcgggg atcgtagtag cggaacacta cgccagctgc
    17221 cgtcagttca ttgacaaact catcgctgag atccggcgaa ccgtagccat ccagcaagac
    17281 ttccgcttta accccgcgtt gcgctgctgc cagtagtgcc gcatgcagct gtttgccgac
    17341 gttatcctca aaccagataa acgtttcaag aatgatgcgt tcttgtgcct cgccaatcgc
    17401 cttaaacacc gcgggataat attgctcgcc gttttccagc aactggatct tattgccttc
    17461 gcgccagcta catttcataa atgaatctcc gcacttaaag gggcatgatc agaaaggtgt
    17521 cgccatgtcc gcagcggcaa cgcggttggc gcgctggcgc tggcattttt gacgtagatc
    17581 ctgtccagtc gtagtagagg aaattgcacc ggaaacgtgc gcgccgggcg tccgtgggcg
    17641 cgggtaaaaa tctcatccag tccggcctgc acttttaacg gatgattagc tttttgccgc
    17701 cagtcattga aatcacccgc caccaatacc ggttcgccgt ccggtagctc attcacccat
    17761 tcggcgagca tcgcaagctg cgcctgacgg tgcgcctcac gcaggcccag atgtacgcac
    17821 atcacatgaa tcgcttttcc ggtcatcggc ggcacaatgc ggcagtagag cacgccgcgc
    17881 ttttccgcac catcgaccga aacatcgcga ttctcataat gttcaatggg ataacgcgac
    17941 agtacggcgt tgccgtgatg cccttccggg tatacggcat tgcgaccgta ggcaaaatcg
    18001 ctccacatag tgtcggcgag aaactcgtag tgcgaggtat cgggccagtt ttccacatgc
    18061 agcggatgaa cttcgtgcgc gcccatcact tcctgcaggc aaacaatatc ggcgctgacg
    18121 gtacgcacgg catcgcgaag ttccggcaaa atgaagcgtc ggttaaacgc ggtaaagcct
    18181 ttgtgaatgt tgatggtgag caccttgaac gaaaattgtt gtgtttgatc gggcataatt
    18241 ttcctgtctt tgcctcttat ctcattgaaa tagtgtagtc ggcgtcacaa aaaggtgcgg
    18301 tcttacggaa ttttccgtaa agttcggtac tctgagtaag tagagataaa ttcttcagga
    18361 gagaagccat gaagtggcaa caacgtgttc gtgtcgcaac gggtctaagt tgctggcaga
    18421 ttatgttgca tttactggta gtggcgctgc tggtggtggg ctggatgagt aagactctgg
    18481 ttcacgtcgg cgtgggatta tgcgcactgt attgtgtcac ggtagtgatg atgctggtgt
    18541 ttcagcgcca ccccgagcaa cgctggcgtg aggtggcaga cgtgctggaa gagctgacca
    18601 cgacctggta ttttggcgca gcgctgattg tgctgtggct gttgtcccgc gtactggaaa
    18661 acaacttttt gctggcaatt gcagggctgg cgatccttgc tggcccggcg gtagtgtctt
    18721 tgctggcgaa agataagaag ttacatcacc ttacgtctaa acatcgcgta cgccgctgac
    18781 cctgtcgtgg ccgttatcac cagtagcggc cacaaactat tccacacaat atccagactc
    18841 gcatccttca aataaatctg cttggtaatg tccgtaaagt ggcgaatagg gttaatccac
    18901 gtcaggtttt gcagccatac tggcatgttt tccaccggag aaacgtaacc ggaaaggaga
    18961 atggcgggca tcataaaaac aaacacgccg ataaacgcct gctgttgtgt cgagcagagt
    19021 gatgaaatca acagaccgaa tcccaccagc gataaaccgt aaatcaccat cgtaaagtag
    19081 aacagcgcca gcgatccggc gaaggggatt tgatacgccc agataccaat cgccagcaca
    19141 atggtggcct ggaaggtggc gacaattaac gccggtacgg ctttgccgat gaagatctgc
    19201 caggtggtga gcggcgaaac cagtagctga tcgagcgtac cttgttcacg ttcgcgggcg
    19261 acggaaagtg aagtgacgat cattacgccg atagtggtga tcatggcgat cagcgacggc
    19321 accacaaacc atttgtagtc aagattcggg ttataccagt tgcgtaccac cagctcgctg
    19381 ttgttaggtt tcggttttcc ttccagcagc tcctgctgat aatttttgac gatctgttgc
    19441 aggtagttgg cggcaatttg cgcactgttg gagttacgcc cgtcgaggat caactgcaac
    19501 ggcgcggtct ggaaggtatc cagtttgcgc gagaagtcag ccgggaaacg caccagtagt
    19561 aacgcctttt gtgtgtcgat ggttgggcgg atctcctgtg ggcttttcag cagcagcaca
    19621 tgggtaaagg cgctggcgcg ggcaaaacgt tgggtcagct ccaccgaatg ctcgccgtta
    19681 tcttcatcgt agatggcgat ggtggcgtta gtcacttcca gcgtggcggc gaacgggaac
    19741 aggatcacct gaataagcac aggtaaaatc agaatcgcgc gggtttgtgg ttcgcgcagc
    19801 aacgactgca actctttgcg gattaacgtc cataagcgat gaaacatgct cttctcccta
    19861 atccagccga cgtttggttt tcagccacgt caggccgata aacatcaccg ccgaagcgat
    19921 caaaaacagc acgtttacca ccagcaccac tggaatattc ccggcgagga acaggctttg
    19981 cagggtgctg acgaaataac gagcgggaat aatgtacgtc accgcgcgga tcaccgcggg
    20041 catactgtcg atctgaaaaa taaagccgga aagcataatc gacggcagga acgcggcgtt
    20101 cagggcgacc tgagcggcat tgaactggtt gcgggtaatc gtggaaatca gcagccccat
    20161 ccccagggta ctgagtaaaa acaggctgga gataaaaaac agaataagca gcgaaccgcg
    20221 atacggcacg ccgagaataa acactgacac cagcatacac agcagcatcg ccagcatccc
    20281 gagaaagtaa taagggatca gcttacacag cagcagttcc gtgcgggtaa tctccgtaga
    20341 gagcagagcc tccatggtgc cgcgttccca ttctcgcgcc accaccagcg aggtgagaat
    20401 cgcgccgatg accgtcatga tgatggtcac tgcaccgggg ataatgaagt gctggctaat
    20461 cgccgccggg ttaaaccagt agcgggtttg tacatcaata agcggttcaa aagtctgccc
    20521 gttgtcctcc gctcgctgca tttgccagat ctgccagatc tgccagatcc cttcgacata
    20581 cccctgtaca aagttagcgg tattcggctc actgccgtcg gtgatcacct gaatcggtgc
    20641 ggtggcgttg gcgcgctcca tctgttccgc aaaatccacc ggaataacca ccagaccgcg
    20701 aattttcccc gcctgcattt tggcgatcag ttcctgacgg ttatcgctga tggtggcgtc
    20761 gatgtagggc gaaccggtca tggtgtgggt gaaatccagc gccgcttcgc tacgctgttc
    20821 cagcaagatc ccgacccgca gcttgctgga gtcgaggtta atgccataac caaaaataaa
    20881 cagcagtagc agtgggatca ctaccgcaat cagccagcta ctcggatcgc gaacgatctg
    20941 ccgcgtctct ttgacgcaca gcgcccgcac gcgacgccag gaaaggatcg ggttactcat
    21001 tgctatgctc cttatcccag tcgtggatca actgaataaa ggcttgctcc atggtgggat
    21061 cgggttgctc atcgttagcc gactgtgctt tcaaatcgtc cggcgtaccg ctggcgatta
    21121 atttcccacg gtacaccagg ccgatgcggt cgcaatattc cgcttcatcc ataaagtggg
    21181 tggtgaccat caccgtgacg cctttctcta ccatgctgtt gatatgtagc caaaattcac
    21241 ggcgggtgag gggatcaacg ccggacgtcg gttcgtcgag aaacagaatg tccggttcat
    21301 gcatcagcga acaggccagc gccagccgct gtttaaaacc taatggcagt tcatcggtgg
    21361 cgtgggaggc gatacttttc aggccgaacg cctcgctcat gcgggagatt ttttcgttct
    21421 gcgcccgacc gcgtaagcca tacacaccag agaaaaagcg taaattctgt tcgaccgtca
    21481 ggttaccgta gagcgaaaac ttttgcgcca tatagccgag atgctggcgc gctttaccag
    21541 aactctcttt cagatccatc cccagtacca gcgcctgacc agaggtcggc accagcaaac
    21601 cgcacatcat cttaaaggtg gtcgatttgc ccgcgccgtt tggccccagc aaaccaaaaa
    21661 tctccccgcg tttaacggca aagttgacgt gatcggtggc ggcaaaatca ccaaatttct
    21721 tggtcagttc tttcgcttcg atcaccgtct cgccgggtgt gccttctacc gtatgtaata
    21781 ttgcgcccag cggcgattcc gaggttccgg caccgcccag caaatcaata aacgcatctt
    21841 caaaacgcgg cgtagtttcg ttgatgttga tttccggcat cccgtcggca tggcgaatat
    21901 cgtctggtgt ggtctctttt ttgaggatca gacgtaccga tttcccctga atcatgccgt
    21961 cgctgacctg cggcagtttc aatgcccgtt gcaacagttt gcggttgccc tcgtgtggac
    22021 ttgtcatcag aaagctgcgt ccggccatgg tttgtgtcag ggcttttggt tccccctgat
    22081 acagcaactc gccttcattc atcagtaaca cgtcacggca ctgctcggct tcgtcgagat
    22141 acgaggtact ccagaggatt aacatccctt cgcctgccag ctcatgcacc atctgccaca
    22201 gttcgcgccg tgagataggg tcaacgccga cgccgggttc atcgagcagc aacactttcg
    22261 gttcgcccac cagggtacag gccaggccga gtttttgttt catcccaccg gagagcttgc
    22321 ccgccaggcg tccggtaaac ggcccaagag acgtaaactc cagcaggcga gcaaaagttt
    22381 gcttacgtgc ctcgccggtg acgctgcgca aatccgcgta cagattgagg ttctccatca
    22441 ccgtgagatc ttcatacaga ccaaatttct gcggcatata gccgagcacg gcgtgcagcg
    22501 cgccgtcgtt tttgattgga tcaaagccaa tcaccgtggc attgccgctg tcgggtttca
    22561 gtaatcccgc cagcatccgc attagcgtgg ttttacctgc accatccggc cccaccagcc
    22621 ccgtcacata accggcgtga atggtacaat cgagcggcgc gacggcgggc ttgtccatgc
    22681 ccggaaagcg tttttccagg ccgttcagcg tgataacggc atcattcatg tcctgcctcg
    22741 ttaccgaatt gcaccgtcac cggcattccc tggcgtaacg catcatcggc gtcggtcacc
    22801 acaatacgca ggcgatagac gaggtcggta cgcagatccg gtgtttcgac ggttttcggg
    22861 gtaaattcag cagtcggcga aacgaaacca atctgcccgt gatacggctt gtccgggcga
    22921 ccatcggtat aaagcagcac tttgcgcccc ggctgggcct ggtcaagata ccgttcatca
    22981 acataagcgc gcacccacac cggacgcgtt agtgaaacgg taaacaccgt gccaccttca
    23041 ttgaggaccg tgcctggctc caccgcgcgc gttaacagcg tgccatcaga cggggctatc
    23101 aacgttgagt cctgtaaatt caactccgcc tgcgccagtt gcgcctgcgc ctgttcgagg
    23161 ctggctttcg cctgggcgat gtcctgttca cggttaccgg aacggtactg acgcaattta
    23221 tcctgtgctg atttcagcgt tgcctgcgcc tggtcgcgcg aggagcgggc attttccagg
    23281 tcatttgccg aaatagtgcg gcttttccac agtccttgct ggcggttata gaagttctgc
    23341 gcatagtcat aggcggcttg cgcctgtttc accgctgcgg cggcctgagc gatttcttca
    23401 tcgcgatacc cggcaagcat cagatcatac tgcgcctgtg ccaccgagac gcccgctttc
    23461 gcctgcatca gggcaatctc atacggcttg tgatccagtt cgcccagcac ctggcccgct
    23521 ttgatagcat caccttcgtc caccgccagc gattcaacgc gccccccaac acggaaacta
    23581 agattcaccg tacgaatatc cacgttgcca tacagcgtca ggccgttatc ctggcggctt
    23641 tgataccacc agtagcctcc ggcaaccacg gcggcaagta ccactaccgc cagttcgatc
    23701 acgacaggtt ttttcatcac tacaaactcc tttgcgataa tccttgcaaa atcaggtcga
    23761 tatgacaggt caccgtctgg ttgatcagtt cggtcttttc ttcatcgaac gcggtccagc
    23821 cggtacgtaa cagaattgtt tctttgccaa gacggaacgc cagaatctca ccaatcaacg
    23881 catgggtatg aaggatcatg cgggtgtcat tggcgtcgct gccggtccag gcggcaatca
    23941 ggcgtgtcag gtggctgtgt agcggactaa tcacctgttc gtgcaccagg tggtaggctg
    24001 ccgtgggaga gagctgctca cgggagataa acttgctgag gttgacggtg tcatcctggg
    24061 tgagcagctt aatcatgttc ctgcaggcgc gaaggagcag ttcacggatg gcagcccgat
    24121 caggctgtgg ttgggcgaac aagcgttcgg cttcctcggc atgcggacgg aactgctcgc
    24181 caataaaatc ggcaatccac tgggcgcagg cgaggtacaa atcttctttc gaaccgaagt
    24241 agtaggtgat ggcagcgata ttctgcccgg cttgggcggc tatctcgcgg gtggtggcgt
    24301 tcattccata ttcaccaaac tgcgccagtg cggcagcaat cagctgtttt ttcgcctgtt
    24361 cacccttgat tgtcatggca ggattattca tcgcacagtc cattcttaat caaatgattg
    24421 attaagatta tgactccata tgggagttgt ccagtatggc taagaatttt agctacgcca
    24481 gtcacaggga taatttatgc gctgcgtcac aaaaactgct acactccgct cgctcatgac
    24541 attgtggttt ttgtcatttt ccttttcagt atctccctga aaactacacc ggtaacggtc
    24601 ggggcggttc ggagtagtca tgtctttcga ttatttgggt ttaagccctg atatcctgcg
    24661 cgccgttgcc gagcagggtt accgtgaacc cacccctatt cagcagcagg cgatccctgc
    24721 ggtgctggaa ggccgcgacc tgatggctag cgcccagacc ggcaccggca aaacagcggg
    24781 ctttacgctg ccgctgttgc aacacctgat cactcgccag ccgcacgcca aagggcgtcg
    24841 tccggtacgt gcgctcattc ttaccccgac ccgtgaactg gcggcgcaga ttggcgaaaa
    24901 cgtccgtgat tacagcaaat acctgaacat tcgttcgctg gtggtgtttg gtggtgtgag
    24961 tattaacccg cagatgatga aactgcgtgg cggcgttgat gtgctggtgg caacgccggg
    25021 acgtttgctg gacctggaac atcagaatgc agtgaagctg gatcaggttg aaatcctcgt
    25081 cctcgatgaa gctgaccgca tgctcgacat gggctttatc cacgatatcc gtcgcgtgtt
    25141 aacaaaacta cctgcgaagc gccagaacct gttattctcc gcgaccttct ctgacgatat
    25201 taaagccctg gcggaaaaac tgttgcacaa cccgctggaa atcgaagtgg cacgccgcaa
    25261 taccgcgtct gatcaggtga cccagcacgt tcactttgtc gataagaaac gcaaacgcga
    25321 attgctgtcg cacatgattg ggaaagggaa ctggcagcag gtgctggtgt ttacccgtac
    25381 caaacacggc gctaaccatc tggctgaaca gctcaataaa gatggcatcc gtagtgcggc
    25441 gatccacggc aataaatcgc aaggtgcgcg tactcgtgcg ctggctgatt ttaaatcggg
    25501 cgatattcgt gtactggtgg caactgacat cgctgcgcgc ggcctggata ttgaagagct
    25561 gccgcacgtc gtcaactatg aactgccaaa cgtaccggaa gattatgtcc atcgtatcgg
    25621 gcgtaccggt cgtgcggctg ctaccggtga agcgttgtcg ctggtgtgtg ttgatgaaca
    25681 caaactgctg cgtgatatcg aaaaactgct gaaaaaagag atcccgcgca ttgcgattcc
    25741 gggctatgag ccggacccgt caatcaaagc cgaaccgatc cagaacggtc gccagcaacg
    25801 tggcggcggc ggtcgtgggc aaggtggtgg tcgcggtcaa cagcaaccac gccgtgggga
    25861 aggtggcgca aaatctgcaa gcgcgaaacc tgcagaaaaa ccgtctcgcc gcctcggcga
    25921 tgccaaaccg gcaggcgaac aacaacgtcg ccgccgtccg cgtaaacctg ccgctgcgca
    25981 gtaatctttt atgccgggct atgcccggca tcaggctgat gaacaaacgc aaaactgcct
    26041 gatgcgctac gcttatcagg cctacgtgaa ctctgcaata tattgaattt gcatgctttt
    26101 gtaggccgga taaggcgttt acgccgcatc cggcatttca caacaagcac ttgtcagcaa
    26161 tttgagaaca cgggaaaaaa tttatctgtt ttaccgccca taagccaccg agacgttact
    26221 tctctatagc caattgttct cgcaactcca ttaaaaggta gcccagtcga ttcttgccct
    26281 taccattacc accgtctccc cagtaagcat cgttttccgt atgctcaacc agttttgcgg
    26341 gcgcggttgc cagcaagagc acacgcagtt ctgcatgctg ttcgaattta gcacgaagtg
    26401 ctttacgcat cacctgttct ttaaccgact cccagttttt acgaagtggc ttggaacgat
    26461 tacgccccat gcgagcggcg accatggggg aagaaacccg acggatctct tcgcggtatt
    26521 tttcatcaag gaatttttgt gcctgaaaat agtgttcgga ggtgggccag gttttcccgt
    26581 caaccttgat gggccaggcg gcaaagttag aaaaatcacc gtagtcatcg ctggtgctgt
    26641 agaaatttat gatggtgtct tgcatgacgt gttggatcct ttgtgctcga acgggcatta
    26701 aaccgcatta tgttggtggt tattgcgagc cgctttccag aaacagaaaa accattaccc
    26761 ctgaaaaccg aaaaatgcca caatattggc tgtttataca gtatttcagg ttttctcatg
    26821 gcattaaccg ccgcgcttaa agcgcaaatt gccgcctggt ataaggcgct tcaggaacag
    26881 atccccgact ttattccccg tgtgccgcag cggcagatga ttgcggacgt cgccaaaacg
    26941 ctggccggag aagaagggcg gcatctggcg attgaagccc ccaccggcgt tgggaaaacg
    27001 ctctcctatt tgatccccgg catcgccatt gcccgcgaag agcaaaaaac gctggtggtg
    27061 agtaccgcca acgtggcgtt gcaggatcag atttacagca aagatttacc gctgctgaaa
    27121 aagatcattc ccgatcttaa attcactgcc gctttcgggc gtgggcgcta cgtttgtccg
    27181 cgtaatctga cggcgctcgc cagcactgaa cccacgcaac aggatctgct ggcgtttctt
    27241 gacgacgaac tgacggcaaa taatcaggaa gagcaaaaac gttgtgcgaa gctgaagggc
    27301 gatctcgaca cttatagatg ggatggtctg cgcgatcata cggatatcgc tatagatgat
    27361 gatctctggc gtcgtttgag taccgacaaa gccagctgcc tcaaccgtaa ctgttactac
    27421 tatcgtgaat gcccgttttt tgttgctcgt cgggagattc aggaagcgga agtggtggtg
    27481 gcaaaccatg cgctggtgat ggcggcgatg gaaagcgaag ccgtactgcc tgacccgaaa
    27541 aatctgctgc tggtgctgga cgaaggtcat cacctgccag atgtggcccg ggatgcgctg
    27601 gagatgagcg ctgaaatcac cgcgccctgg tatcgactac agctggactt gttcacgaaa
    27661 ctggtcgcta cctgcatgga gcagtttcgc ccgaagacca tcccaccgct ggcgatccct
    27721 gaacgtttga atgcgcattg tgaagagttg tatgagctta tcgcctcgtt aaacaacatt
    27781 ctcaatctct acatgcctgc cgggcaggag gcagagcacc gttttgcgat gggcgaactg
    27841 cctgatgaag tgctggagat ctgccagcgg ctggcaaaac tcaccgagat gctgcgtggt
    27901 ctggcggagt tatttcttaa cgatttaagt gagaaaaccg gcagccatga cattgtacgc
    27961 ctgcatcggt tgattttgca gatgaaccgc gcgttgggga tgttcgaggc gcaaagcaaa
    28021 ctctggcggc tggcgtctct ggcgcaatct tccggtgcgc cggtgaccaa atgggcgacg
    28081 cgggaagagc gcgaagggca actgcatctc tggtttcact gcgtggggat acgtgtcagc
    28141 gatcagctgg aaaggctgct gtggcgcagt attccgcaca ttattgtcac ctccgcaacc
    28201 ttgcgttcgc tgaatagttt ttcgcgtttg caggagatga gtggtctgaa agagaaagcg
    28261 ggcgaccgtt ttgtggcgct ggattccccc tttaaccact gcgaacaggg caaaattgtt
    28321 attccccgga tgcgcgttga gccttccatc gacaacgaag agcagcatat tgccgaaatg
    28381 gcggcctttt tccgtaagca ggtggagagc aaaaaacatc tcggtatgtt ggtactgttt
    28441 gccagcggac gggcgatgca gcgctttctc gactatgtga cggatttacg tctgatgttg
    28501 ctggttcagg gcgatcagcc gcgttaccgt ttagttgaac tgcaccgcaa acgcgtcgcc
    28561 aacggtgagc gcagcgtgct ggtgggctta cagtcatttg ccgaagggct tgatttgaaa
    28621 ggtgatctgc tcagccaggt gcatatccac aaaatcgctt ttccgcccat cgacagcccc
    28681 gtggtgatca ccgaagggga atggctgaaa agcctcaacc gctatccgtt tgaggtgcaa
    28741 agcctgccga gcgcctcgtt taacctgatt cagcaggttg ggcgactgat tcgaagccac
    28801 ggttgctggg gcgaagtggt tatctacgac aaacgcttgc tgaccaaaaa ttatggcaag
    28861 cgactactgg atgcattacc ggtatttccg atagagcaac cggaagtccc tgaaggtata
    28921 gttaaaaaga aagaaaaaac gaaatcccca cgccgtcggc ggcgttaatg atgtgagtca
    28981 ggtaaggagt cgtaaatgga ctatcgcaaa atcattaaag agatcgggcg cgggaaaaac
    29041 cacgcgcgcg atttagaccg ggatactgcg cgcggtctgt atgctcatat gctcaatggt
    29101 gaagtccctg accttgagtt gggcggcgta ttgattgcgc tgcgtatcaa aggagaaggg
    29161 gaagcagaga tgctcggctt ttacgaagcc atgcaaaatc acaccatcaa gctgacgccg
    29221 ccagcaggca agccgatgcc gattgtcatc cccagttaca acggcgcgcg taaacaggcc
    29281 aacctgacgc cgttgttggc gattctcctg cacaaactcg gttttcctgt ggtggttcac
    29341 ggggttagcg aagatccaac ccgcgtgctg actgaaacca tttttgaatt gatgggtatt
    29401 acgccaacgt tgcacggcgg gcaggcgcag gcgaagctcg acgaacatca accggtgttt
    29461 atgccagtcg gcgcgttttg cccgccgctg gaaaaacaac tggcgatgcg ctggcggatg
    29521 ggcgtgcgta acagtgcaca taccctggcg aaactggcga cgccatttgc cgaaggtgcg
    29581 gcgctgcgtc tttccagcgt ttcgcatccg gaatatattg gacgcgtcgc gaagttcttt
    29641 agcgatatcg gcgggcgggc gctactaatg catggtactg aaggtgaagt gtatgctaat
    29701 ccgcagcgct gcccgcagat caatctcatt gaccgtgaag ggatgcgggt gctgtatgaa
    29761 aaacaggaca ctgctggtag cgagttactg ccacaagcaa aagatccgga aaccacggcg
    29821 cagtggattg agcgttgcct tgctggcagc gaaccgattc ccgaatcgct gaaaatccag
    29881 atggtttgct gcctggtggc tatgggtgaa gcggcaacta tcagcgacgg cctggcgcgc
    29941 gttaatcagg cattttaatt cttttctcag ccggatgagc catgtcatcc ggcttttacc
    30001 ccaccgcaat atgaaattcc tgcatcttta ttgaccttcc cacgcccggc gtgcagcata
    30061 aaaatacaac aaacacataa cataaacagg agttaaccat ggaaagtggt catcgctttg
    30121 atgctcagac gctgcacagt tttattcagg ctgtatttcg tcagatgggt agcgaggaac
    30181 aagaagcgaa attagttgcc gatcatttaa tcgcggcaaa cctggcaggg catgattcac
    30241 atggtattgg catgatccca agctatgtac gctcctggag tcaggggcac ctgcaaatta
    30301 accatcatgc caaaaccgtt aaagaggcgg gggcggcggt cacgctcgat ggcgatcgcg
    30361 catttggtca ggtcgcggca catgaagcga tggcgctggg gattgagaaa gcgcatcagc
    30421 acggtattgc cgccgtggcg ctacataact cgcatcatat cggccgtatc ggttactggg
    30481 cggagcagtg tgcagcggcg gggtttgtct ctatccactt tgttagcgtg gtcggtattc
    30541 caatggtcgc gccgttccac ggtcgcgaca gccgctttgg caccaatccg ttctgtgtgg
    30601 ttttccctcg taaagataat ttcccgctgt tgcttgatta cgccaccagc gccattgcat
    30661 ttggcaaaac ccgcgtcgcc tggcataaag gcgtccccgt gccgccaggt tgcctgattg
    30721 acgttaacgg cgtgccgacg accaatccgg cggtaatgca ggagtcgccg ttgggttcgc
    30781 tgttgacctt tgccgaacat aaaggctacg cccttgcagc gatgtgtgaa attcttggcg
    30841 gggcgctttc cggcggtaaa acgacgcatc aggaaacgtt acaaaccagt cccgatgcca
    30901 ttcttaactg catgaccact atcatcatca acccggaact cttcggcgcg ccggattgta
    30961 acgcgcagac cgaagccttt gccgagtggg tgaaagcctc gccacatgat gatgataagc
    31021 cgattttgct accgggcgag tgggaagtga acacgcgtcg cgaacggcag aagcagggga
    31081 ttccactgga tgcgggaagc tggcaggcca tttgtgatgc agcgcggcag attggtatgc
    31141 cggaagagac gttgcaggct ttctgtcagc agttagccag ctaaaaaaaa gcccgtccag
    31201 tggcggacgg gcaaacaagg gtaacatagg atcaatgagg gttagagcat atgcgtctgt
    31261 cggcaaacag acagggaaat acttgtgctg gacgtagcgt aaacgcctga tccgtccaac
    31321 ggttcggatt ttgtaggcct gataagacgc ggtagcgtcg catcaggcat ctgttgccgg
    31381 atgcggcgta aacgccttat ccgtcctacg aattacttat aaattaccgc agtaccgctt
    31441 aatttattgt tgttggtggc ggaagtgatg ctgtatccac tggcaccggc tgccgcggct
    31501 ttctcagcca gtttcgcttc cagggcatcg agggtggatg cgccatcggc agaaaccacg
    31561 ccgattttat tcatgttctg tgcctgggat gccgttatcg gttccgccgc gaacacgcca
    31621 aatgacagag ttgaaagagc catagcagca acaacagtat tgatagtttt cataattaat
    31681 ctctcgcagg tgatttttgt ataagtgacg ttgtttcgtc gatgtgatga gtatcacgtt
    31741 tttttacgag agataaaatc gaagagaatt gaccgccttg ttcaaataaa ttgattgata
    31801 tctaacatac tgataaacca tttatttgtt gtacttatga acttgtttta taccgcgtgg
    31861 cagtcacagc agcgttaaag gcacggaatg ccacgcaaaa taaaaattat actcgctatg
    31921 catctcgcca gttgtagtta acgtaactgg ctgtctttcg aacctctgcg attatatcct
    31981 gtataagctg gtttttgtaa atctttctcc tgctgacaat gaatacataa gcgcacgcca
    32041 ggaatggctt cccgacgggc ctgcgggatg ggggcaccgc actcttcaca ttcatccagg
    32101 ctttcgccgc gcggaatttc accccgagcg cgggcaatcg cattttcaat tgtactgttg
    32161 atctgttcgt tgacggcgtc atcgttagcc caaccggatg ccatatcgac ctccccatat
    32221 caatacttgt acagttaagt gtagctaatc cagggacgaa ctcgggcagt tcaagcatca
    32281 gatctccgac cattcccgca gcagattatg ataaagatta agcagcgaca ggatctcttc
    32341 actttcgccg tagcggcttt tcagcgactg aatattgttg tccagttcaa acagcatggc
    32401 gcgcttttta tcatcgcgga tcatcgactg gatccacata aatgatgcca ctcgtacgcc
    32461 gcgggttacg ggtggcacgc aatgctggct gctggaggga tacaacacga gatcgcctgc
    32521 cgggagtttt acccgatgtt gtccgaaggt gtcattaacg accagttcgc cgccgtcgta
    32581 gctttgtgga tcgcttaaaa acagcgtggc agaaaggtca gtacgcatcc agccgttttg
    32641 tggatggctg cgtactgcgc catccacatg gaaaccatag gtttcattgt tctgatagcg
    32701 attaaacagc ggcgtggaaa gggtacgcgg caaggccgcg gcaaagaaca aagcatgttg
    32761 gttaaccgcg ttcagcacct cattttgcag ggcggcgtat aacgtgctgc gggtgtcgac
    32821 ctgttgattg ttcttaactt gcgcaccttg tgcgccggtg gtgacgcgtc catccaccca
    32881 ttcggcttgt tccagttgtt cgcgaaaccg ggcgacgtcc tgtggcgata acacgccggg
    32941 aatgtggtac atcatacaag ttctccaaaa agtggggcct gcgccccaca tctgaatcag
    33001 aaatgcatat tggctgtgag caagaaggtt cttggctcgc ccgggtgata acggtagccg
    33061 ctcttgttga ttgaggcgac gtaatcagta tcaaacaggt tgtagacgtt tagctggaag
    33121 tcgagattgc gattaacccg ataccccagt ttggcatcgg cgacccagta accttcggta
    33181 aatgctggcg ttcccaccgc gccgtctgaa cctttatgca tactgccgat atagcgtgcg
    33241 cccgcgccaa cagagatatc gtcggttgcc tgatattggc tccataaggt gaaggcgtgc
    33301 tccggggtat acggcagcga tgaggaacca tcctgggcaa catctttgcc gtttttgatg
    33361 gttgcttttt gctgggtata gccgccaatc acctgccacg cgggagtgat attcccggcc
    33421 acggatatct catagccttc gacgcgtttc ttaccgtatt gcgagtaagt tccgtcatca
    33481 ttttgctcaa cttcattttc gatatcagta cggaacagcg cggcggtgag caacagacgt
    33541 ttatccagaa cctgccattt ggtgccaatc tcgctggtgt tggctttttg cggtttaaaa
    33601 tcggtgcggt tggcactgtt accgctgcca gactgcgcaa gggcgaagtt gttgccgccc
    33661 ggaggctgct gggaaacggc atagttaata tagacattgc cgttttccgt caggtgatac
    33721 agcgccccgg ctttccagtt catcagattg cccgacttgg cggtgtcgac ggtggtgacc
    33781 ggagaacctt ttgccacacc agttgggcag gtgatggcac cgcgtccgct gccgccgcag
    33841 gcggtggcac tgtcatattc agtatgataa ttatccagac ggatcccgcc gttcagctca
    33901 aaatcacggg tgatttgcag cgtatcaaag gcgtaaattg cgaaggtatc cgtctgacca
    33961 ttggcgtttg cgccgttgcg cgtcaggccg ccgggatgaa tgctgctgtc aggatgataa
    34021 atatttaccg cgggtaacgt caccggatta acgccgtagt tagtctgcgt ttcacgggta
    34081 aattccacgc cggtactgac atcatgaccg atagaaccgg tatagaacgt cgaggtcagg
    34141 ttggtctggt tggtgagaat tttattactc acatctttgg tattcgccgt gcgtgaccag
    34201 gttcagctat tcacatcgct ggtgggctga gtaatattcg acgccccgcc cataatcgcc
    34261 gtcatcaggt aatcctgctt tacgcgcgac caacgggtag tattgcgaat ggtggtgtta
    34321 tcgttgatgt cgtgctcaaa acgcatggtg gcggtgtcgg tggtcgaatc gtcgtaatcg
    34381 gaatccgtgc cgtaaaagtt atgagtatca acttttccgg aatgattcag ggccgccgtt
    34441 cccgcagatg gggcagaata gcccggcaaa ccgatggtcg gaatgccgcc gtctggcgtg
    34501 ttgtgctggg tgacatgcag ataattaaga tacaaacgat tcgctgtacc aaggccaaaa
    34561 gcgacagaag gggcgacgcc gtaacgctca tttttgactt tgtcgcgtcc ggcatcgtgc
    34621 gttttttcgc ccattacatt caggcgcacc gcggtggtat caccaatgac ctgattgacg
    34681 tccagcgtgc cgcggcggaa ccaggcgctg ccaatactgg cggaggcgtc aatgccggaa
    34741 tcattgcgcg gctgtttgct gatcatattg atcgagcccg tcggtgcgct gcgcccgtag
    34801 tcggtgccgg acggcccttt aatcacttcg acctgctcgg tattgaaggt gtcgcgcgag
    34861 acgctgccga tatcgcgaat gccatcaata taaatactgt tagaggtatc ggcaccacgc
    34921 atataaatgg cgtcgccagt ggtggagtta ccgttctcac ccgcaaaaaa cgcacccacg
    34981 c
//
LOCUS       DAZPDU010000025        34963 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZPDU010000025 DAZPDU010000000
VERSION     DAZPDU010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34963
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.025"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            293..766
                     /locus_tag="ACHURS_001104"
     CDS             293..766
                     /locus_tag="ACHURS_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Panacea domain-containing protein"
                     /protein_id="HFR1236892.1"
                     /translation="MYSPVQIANKFITLGNQHHNPLTHMQLQKLTYIAHGYYLALTGK
                     PLLNECVSAWKYGPVIPGMYDAFKDYGNKPVTNVAVAPFGGIVTMDPQAESIIGAVYK
                     FYGSKNGIELSTLTHMPGTPWSQAYNGIGSSIISNDAIKAYYHDLLNNRQQCQGL"
     gene            789..1337
                     /locus_tag="ACHURS_001105"
     CDS             789..1337
                     /locus_tag="ACHURS_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1236893.1"
                     /translation="MSHNSDIYKLIGAAAGVENGRSEASLNSTDSQEQAFESAFEPSN
                     HERDDDSSSENKAILEAEEFGSNTGALHEFMQQNRMDSLQAQLDMLKSQVRDKIADAT
                     GKEIDNELRTKMASFTVWFMSCWCLFVAGMFVSFLIAHEGKPPVEAIVALLGTSTISI
                     VGLVGFVVSGLFKSRKDGDKEK"
     gene            complement(1371..1643)
                     /locus_tag="ACHURS_001106"
     CDS             complement(1371..1643)
                     /locus_tag="ACHURS_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyocin activator PrtN family protein"
                     /protein_id="HFR1236894.1"
                     /translation="MNTLFLLMAEFNTPNIELSAVSQKYFGMSPATAEAKANACKLPV
                     PTYRIGTSQKAKRCINIQDLAEYIDKRREEGRAEWEKVRTDKQKYN"
     gene            complement(1680..2252)
                     /locus_tag="ACHURS_001107"
     CDS             complement(1680..2252)
                     /locus_tag="ACHURS_001107"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFR1236895.1"
                     /translation="MNNLMIDLETMGKNKDAPIVSIGAVFFTPETGDIGQEFYAVVSL
                     DSAMKQGATPDGDTILWWLKQSPEARAAICIDDTLSISDALSELNHFINRHADNTKYL
                     KVWGNGATFDNVILRGAYERAGQICPWAYWNDHDVRTIVTLGRSIGFDPKMDMPFDGE
                     RHNALADARHQAKYVSAIWQKLIPATSTEL"
     gene            complement(2252..2749)
                     /locus_tag="ACHURS_001108"
     CDS             complement(2252..2749)
                     /locus_tag="ACHURS_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFR1236896.1"
                     /translation="MAWDMALGWIIWCHRAAMLQGSQPVSQTYNLPELIEGMEVSIDV
                     STCDADLGNRYFGTVTEALELDTAKNGYILLVQDAEPNFDVNGNSPGTPDSWISCSDR
                     MPEKGQNVLISVNFDSSLVEPLICAARYTGSTFRRGDATIKPGNGIEQATHWMPLPEP
                     PQEVK"
     gene            complement(3007..3198)
                     /locus_tag="ACHURS_001109"
     CDS             complement(3007..3198)
                     /locus_tag="ACHURS_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1236897.1"
                     /translation="MNGQISIVRPGACDDCEIRMIIRLARGKTITALITPENLALALT
                     GKSDLPVELKLRNIEIKVK"
     gene            complement(3200..3799)
                     /locus_tag="ACHURS_001110"
     CDS             complement(3200..3799)
                     /locus_tag="ACHURS_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001609235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFR1236898.1"
                     /translation="MSEIDYQALRERYSPKPVPECSVCGEEMSIQRISGAHVTYACSG
                     YGGDGDFKIGRTLADEHYEKSRVTVVDDSDPDVIALLDELEAAKSKLNEQREYYEGVI
                     SDGSKRIAELEAREIKPAKGEVLVVVSGFTGCGKSAIAGEIEIAMKAIGVPVQWTNGD
                     AEKHMTGADWLTAIEMYKPNVRIVEVNVSRAAGIRIKGE"
     gene            complement(3796..4281)
                     /locus_tag="ACHURS_001111"
     CDS             complement(3796..4281)
                     /locus_tag="ACHURS_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR1236899.1"
                     /translation="MTGHAAILDMCCGSRMFWFDKNDDRAIFSDIRKEEHTLCDGRRL
                     IISPDLIADFRALPFADASFSMVVFDPPHLERVGDNAWMGKKYGRLNKDTWRDDLRQG
                     FKEAFRVLWPYGVLIFKWNETQIPVRQILALTDRKPVIGQRTGKGDKTHWIIFMKEGN
                     Q"
     gene            complement(4278..4628)
                     /locus_tag="ACHURS_001112"
     CDS             complement(4278..4628)
                     /locus_tag="ACHURS_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001242737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eae protein"
                     /protein_id="HFR1236900.1"
                     /translation="MRMNVFEMEGFLRGKCVPRDLKVNETNAEYLVRKFDEVRAEARN
                     EGINYTASRLAAAFNHGFINKPLAEVFDVTRMILSAKEELANESHPIDGLSGEYAEKS
                     LEEWAERLRKGGSQ"
     gene            complement(4619..5155)
                     /gene="yfdR"
                     /locus_tag="ACHURS_001113"
     CDS             complement(4619..5155)
                     /gene="yfdR"
                     /locus_tag="ACHURS_001113"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFR1236901.1"
                     /translation="MSFIKTFSGKHFYYDKINKDDIVINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFKVIPLSPGHA
                     YGMFMERFNELSELRKCA"
     gene            complement(5284..6108)
                     /locus_tag="ACHURS_001114"
     CDS             complement(5284..6108)
                     /locus_tag="ACHURS_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HFR1236902.1"
                     /translation="MSQNLDATAINQIHALISAQGVNEIISKIGADAVALPENFRIHD
                     LEKFNLNRFRFRGALSTASIDDFTRYSKDLADEGTRCFIDADNMRAVSVLNLGTIDEP
                     GHADNTATLKLKKTAPFSALLSVNGERNSQKSLAEWIEDWADYLVGFDANGDTIQATK
                     AAAAVRKITIEANQTADFEDNDFSGKRSLMESVEAKTKDIMPVAFEFKCVPFEGLKER
                     PFKLRLSIITGDRPVLVLRIIQLEAMQEEMANEFRDLLVEKFKDSKVETFIGTFTA"
     gene            complement(6174..6536)
                     /gene="yfdP"
                     /locus_tag="ACHURS_001115"
     CDS             complement(6174..6536)
                     /gene="yfdP"
                     /locus_tag="ACHURS_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdP"
                     /protein_id="HFR1236903.1"
                     /translation="MASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMNTKTKGK
                     VSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTILQEDQG
                     QLLTLAGEPDGKLRAAGH"
     gene            complement(7223..7897)
                     /locus_tag="ACHURS_001116"
     CDS             complement(7223..7897)
                     /locus_tag="ACHURS_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional repressor"
                     /protein_id="HFR1236904.1"
                     /translation="MKTVHDIRRSNARKLRDGVGGNSSFATMIDREPTQTSRFMGDGA
                     TKNIGDSMARHIEKCFDLPVGWLDQEHQTTNITKKPDVSITNKQITLVPVISWVQAGA
                     WKEVGYSEVDLSTAETYPCPVPCGEMTYILRVIGDSMIDEYRPGDMIFVDPEVPACHG
                     DDVIALMHDTGETTFKRLIEDGTQRYLKALNPNWPEPYIKINGNCSIIGTVIFSGKPR
                     RYKIKA"
     gene            7988..8188
                     /locus_tag="ACHURS_001117"
     CDS             7988..8188
                     /locus_tag="ACHURS_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
                     /protein_id="HFR1236905.1"
                     /translation="MKAYWDSLTKEQQGELAGKVGSTPGYLRLVFNGYKKASFVLAKK
                     LEQCTSGAITKSDLRPDIYPKD"
     gene            8232..8783
                     /gene="ymfL"
                     /locus_tag="ACHURS_001118"
     CDS             8232..8783
                     /gene="ymfL"
                     /locus_tag="ACHURS_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HFR1236906.1"
                     /translation="MGKHHWKVEKQPEWYVKAVRKTIAALPGGYAEAAEWLDVTENAL
                     FNRLRADGDQIFPLGWAMILQRAAGTHYIADAVAQSAGGVFVSLPELEEVENADINQR
                     LLEVIEQIGSYSKQIRSAIEDGVVEPHEQTAINDELYLSISKLQEHAALVYKIFCAPE
                     KSDARECAAPGVVAFCVCGETNA"
     gene            8929..9108
                     /locus_tag="ACHURS_001119"
     CDS             8929..9108
                     /locus_tag="ACHURS_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFR1236907.1"
                     /translation="MQELNRWFRDGRGLYVHVIRWEPETQRVIYLRKDYPHECFSPLW
                     KFRRDFVECEGPPAH"
     gene            9038..9616
                     /locus_tag="ACHURS_001120"
     CDS             9038..9616
                     /locus_tag="ACHURS_001120"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HFR1236908.1"
                     /translation="MSALVLCGNSGVILLSVKDHQHIDSAIPGRYTVQAPYKAGVGIG
                     VLEFNIEHNRAHAVFSYHEHCYVQIMVGRAGASQDAPGSMLTGYANPVRLTTSVIGVP
                     CGEFFEFNIGAVTMTTLPTLARLNDEDLHKLSYVTTALRALRKVTLSDPQAHQVLVET
                     LLNLQAERIRLADKANFHIHRLLNISGGHRHA"
     gene            9609..9845
                     /locus_tag="ACHURS_001121"
     CDS             9609..9845
                     /locus_tag="ACHURS_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1236909.1"
                     /translation="MLNPLILNICRLLQRKKTSIPTVGQWYTTPAGHVLRVSLVDREC
                     QKVICEPLGRNYRVSMPLITFRSGKNMKHLGGAA"
     gene            9842..10660
                     /locus_tag="ACHURS_001122"
     CDS             9842..10660
                     /locus_tag="ACHURS_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1236910.1"
                     /translation="MSMELMVKAMKIRVGNPLRKLVLIKLADNASDQGECWPSYQHIA
                     DQCEISKRSVMNHIAALCESGLVKKVTRKGEKGNSSNIYLLHLDGAGDSLGGSANNSL
                     SGAANSPGSAGVAPGGSAGDSPRTSHSFEPVKEPVNEPIAVGASADESVRVRSNRPEY
                     SPEFEQAWLAYPKRAGGNSKSAAFKAWKARLNEGVNPETMLEGVKRYAGWVSAMGNSG
                     TQFVKQAVTFFGPDRHFEESWEVPAVSAARREDPYFKASYDNVDYSQIPAGFRG"
     gene            10657..11151
                     /locus_tag="ACHURS_001123"
     CDS             10657..11151
                     /locus_tag="ACHURS_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFR1236911.1"
                     /translation="MIMSLLNDVQKFIEAHPGCISGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHRCEGDTRRHFPRLTERAQEPEPQPVRETRPVRNFYVGTNDPRVILCLTRQA
                     EELESRGLYRRAATVWMAAFRESHSQPERNNFLARREQCLRKSSKRAVSGDEWYLSGN
                     YVGA"
     gene            11151..11804
                     /locus_tag="ACHURS_001124"
     CDS             11151..11804
                     /locus_tag="ACHURS_001124"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFR1236912.1"
                     /translation="MSNKYCQELVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASSMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPAWFIPKDE
                     KQMPTGAFFAGAIAVFDKTWKGPAISYIGRDELEACGEAFLAQVRQQAEKLVREMAA"
     gene            11801..12127
                     /locus_tag="ACHURS_001125"
     CDS             11801..12127
                     /locus_tag="ACHURS_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HFR1236913.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEAVGIIRALLAGEENARLRAAHWLH
                     ERGLKV"
     gene            12124..12513
                     /locus_tag="ACHURS_001126"
                     /pseudo
     CDS             12124..12513
                     /locus_tag="ACHURS_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
     gene            12533..13342
                     /locus_tag="ACHURS_001127"
     CDS             12533..13342
                     /locus_tag="ACHURS_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFR1236914.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAVASGANARTKEPGKF
                     LSSQQTVELVHELTNTQNLGVDPVSVIHGGNERGTYVCKELVYAYAMWISPSFHLKVI
                     RTFDMVTSAPEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNL
                     APRYAIDAPADAHDGSSRPTLSLSALLKQYGIRLTANQAYHQMVKLGIVEQRERYSRT
                     AINNIKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            13350..14339
                     /locus_tag="ACHURS_001128"
     CDS             13350..14339
                     /locus_tag="ACHURS_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFR1236915.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADEPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLIRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLA"
     gene            14357..14728
                     /locus_tag="ACHURS_001129"
     CDS             14357..14728
                     /locus_tag="ACHURS_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFR1236916.1"
                     /translation="MRDIQMVLERWGAWVANNHEDVEWSSVAAGFKGLIPSKVKSRPQ
                     CSDDDGLIISSAMTVLKKKEPYQYELLEMYYVYGVTLRALGVKLGISLNQVVIRLQKA
                     EGFIDGCLAMLGVSLEIDCYI"
     gene            14804..15421
                     /locus_tag="ACHURS_001130"
     CDS             14804..15421
                     /locus_tag="ACHURS_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1236917.1"
                     /translation="MDKNVEHVLVDAIENKQSLTVVYLGGSQPGTLRNISPISINGDK
                     LRARCHSSGAVKVFNLGKIQLPSDPCAVSMHYGDLEVKAYETMQSVNDNFHALYPEGR
                     WGVDFNEHRFALFDFFKNGKRKKTAFMAIEFRERDEEKIITGVTIDIGISGTVISEKS
                     RIPKRRPWVVVGPEHGEYSTYSTLDKAATAFFERLSLIASGLEDN"
     gene            15698..15901
                     /locus_tag="ACHURS_001131"
     CDS             15698..15901
                     /locus_tag="ACHURS_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1236918.1"
                     /translation="MLKQQDMTETAAAVLHFLPADKWVTPRMMTRTTGVSEARCQLIL
                     TQLVLAGLAKDNGGYGNKFRRCQ"
     gene            16052..17104
                     /locus_tag="ACHURS_001132"
     CDS             16052..17104
                     /locus_tag="ACHURS_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFR1236919.1"
                     /translation="MKNTVKINSVDLINADCLHFIQSLPDDSIDLIVTDPPYFKVKPN
                     GWDNQWKGDEDYLKWLDHCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFNVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERVLFAEHYQGPYRGKSDGYAAKERELKQHIMA
                     PLISYFRDARAELGITAKQIAEATGKKNMVSHWFGASQWQLPNEADYRKLQALFSRIA
                     AEKFQEQQLEQPHHQLVASYDSLNRKYSELLDEFKSLRRYFSVSVSVPYTDVWMHKPV
                     QFYPGKHPCEKPADMLRQIINASSRPGDLVADFFMGSGSTIKAAMALGRRALGVELES
                     ERFNQTVKEINELVGK"
     gene            17172..17387
                     /gene="essD"
                     /locus_tag="ACHURS_001133"
     CDS             17172..17387
                     /gene="essD"
                     /locus_tag="ACHURS_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFR1236920.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            17392..17742
                     /locus_tag="ACHURS_001134"
     CDS             17392..17742
                     /locus_tag="ACHURS_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HFR1236921.1"
                     /translation="MTQNYELIVKGICNFENKVTVTLALRDKKRFDGEIFDLDISLDR
                     VEGAALEFYEAAARRSIRQVFLDVAAGLCEGDEQSPEKRPVILDAQNVWITYKGKLPG
                     RITGSLKTPPESQP"
     gene            17806..18339
                     /locus_tag="ACHURS_001135"
     CDS             17806..18339
                     /locus_tag="ACHURS_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFR1236922.1"
                     /translation="MNAKIRYGLSAAVLALIAVGAPAPDILDQFLDEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVFPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            18336..18803
                     /locus_tag="ACHURS_001136"
     CDS             18336..18803
                     /locus_tag="ACHURS_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR1236923.1"
                     /translation="MSRVAAIIYALVICIIVCLSWAVNHYRDNAIAYKEQRDKKVSEL
                     KQATATITDMQQRQRAADVLDAKYTKELADAKAENDALRRKLDNGGRVLVKGKCSVPS
                     SAETSSASGMGNDATVELSPVAGRNVLGIRDGIIRDQTALRTLQEYIRTQCLR"
     gene            18791..18943
                     /locus_tag="ACHURS_001137"
     CDS             18791..18943
                     /locus_tag="ACHURS_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1236924.1"
                     /translation="MPSMIAIILLIILHIWLCRQGGDHFWSESRLNISLLMLEVEHLA
                     RGKGLR"
     gene            19619..20110
                     /locus_tag="ACHURS_001138"
     CDS             19619..20110
                     /locus_tag="ACHURS_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFR1236925.1"
                     /translation="MDHELKNLVLNINQLAALSGLHRQTVVARLKNIRPAGGHDKLKL
                     YRLTDILTEFMGLPPPVAEGEMDPHERKAWYQSERERLKFEQETAQLIPASDVRREFA
                     IWAKAVVQVLETLPDILERDCGLQPAAVSRVQSIIDDLRDQIALRVTEAGADDEEELQ
                     QEE"
     gene            20110..22212
                     /locus_tag="ACHURS_001139"
     CDS             20110..22212
                     /locus_tag="ACHURS_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFR1236926.1"
                     /translation="MLNQETAKAARTDSGYILRAPRRMLVADAVAQYMRVPMGAGNSV
                     PWDPLVAPYVIEPMNCLASREYDAVIFVGPARTGKTIGLIDGWVIYNVICDPADMLII
                     QMTEEKAREHSKKRLARTFRVSPEVVSRLSPNKNDNNVYDRTFLAGNYLKIGWPSVNI
                     MSSSDYKCVALTDYDRFPEDIDGEGDAFSLASKRTTTFMSSGMTLVESSPGRDVKDVK
                     WRRTSPHEAPPTTGILSLYNRGDRRRWYWPCPHCGEYFQPCGDVVAGFRDIADPVLAS
                     EAAYIQCPSCSGRIMPEQKRELNGRGVWLRDGESINADGSRYGDPRRSRIASFWMEGP
                     AAAYQTLSQLVYKLLTAEQEYETTGSEETLKTVINTDWGLPYLPRASMEQRKSELLEQ
                     RAEPVPSRSVPDGVNFLVATVDVQAGRHRRFVVQVTGYGSRGERWIIDRYNITQSLRG
                     DSDGESQRIDPASYPEDWDVLLTDVFHKSWPLASDPSQQMRLMAMAVDSGGEDGVTDN
                     AYKFWRRCRRDGLGKRIYLFKGDSIRRAKLITRTFPDNTGRTGRRAQAAGDVPLWLLQ
                     TDALKDRVNNALWRDSPGPGYVHFPDWLGSWFYDELTYEERSSDGKWSKPGRGANEAF
                     DLMVYAEALVILHGYEKIRWPDAPEWASRETWLECVPDSTEPSLSPEPVSTPVKKQKR
                     KKTVTDDVNPWLTSGGWL"
     gene            22209..22421
                     /locus_tag="ACHURS_001140"
     CDS             22209..22421
                     /locus_tag="ACHURS_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1236927.1"
                     /translation="MNQNDIEAMIQRYTEAEMAVLDGKSVTFNGQQMTMENLSEIRQG
                     RQEWERRLAALITRRRGHPGYRLARF"
     gene            22349..23929
                     /locus_tag="ACHURS_001141"
     CDS             22349..23929
                     /locus_tag="ACHURS_001141"
                     /inference="COORDINATES: protein motif:HMM:TIGR01539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR1236928.1"
                     /translation="MGAPPCGSDYTTTGASRVPAGEVLMAILDDVIGVFSPGWKAARL
                     RSRAVIQAYEAVKTTRTHKARRENRTADQLSQYGAVSLREQARYLDNNHDLVIGVFDK
                     LEERVVGKNGIIVEPHPVLRNGAIARDLAAEIRTRWSEWSVSPEVTGQFTRPMLERLM
                     LRTWLRDGEVFAQMVSGRINSLTPSAGVHFWLEALEPDFIPMTSDESNRLNQGVFVDD
                     WGRPEKYLVYKSRPVSGRQMETKEVDAERMLHLKFVRRLHQMRGTSLLSGVLIRLSAL
                     KEYEDSELTAARIAAALGMYIRKGDGQSYEPDGNGSKENERELTIQPGIIYDDLKPGE
                     EIGMVKSDRPNPNLETFRNGQLRAVAAGSRLSFSSTARNYNGTYSAQRQELVESTDGY
                     LILQDWFIGAVTRPMYRAWLKQAVASGVIRLPRDLDRSSLYTAVYSGPVMPWIDPVKE
                     AEAWKIQIRGGAATESDWVRAGGRNPDDVKRRRKAEIDENRKLDLVFDTDPASDKGGS
                     SAATKRQEPQHTDDQSEE"
     gene            23874..25901
                     /locus_tag="ACHURS_001142"
     CDS             23874..25901
                     /locus_tag="ACHURS_001142"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HFR1236929.1"
                     /translation="MPQRNDRSRSTPTTSPKNNSWFRMQAGHQSDADIYIYDEIGFWG
                     VTAKQFISDLNALGDITHINLHINSPGGDVFEGIAIFNALKTHGASITVYVDGVAASM
                     ASVIAMVGNPVIMPENTFMMIHKPFGFTGGDAEDMRTYADLLDKVEAVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAHGFADQVTPAVKAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRVQDDEPAASRTPVQAAAPVVDENSIRAQVLAEQKARVNGINDLFAMFG
                     GRYQTLQAQCLADPECSLEQAREKLLNEMGRESTPSNKNTPAHIYAGNGNFVGDGIRQ
                     ALMARAGFEKTERDNVYNGMTLREYARMSLTERGIGVSSYNPMQMVGAAFTHSTSDFG
                     NILLDVANKAILQGWEDAPETYEQWTRKGQLSDFKIAHRVGMGGFSALRQVREGAEYK
                     YVTTGDKQATIALATYGELFSITRQAIINDDLNMLTDVPMKLGRAAKSTIADLVYAIL
                     TSNPKISTDNVSLFDKAKHANVLESAAMDVASLDKARQLMRVQKEGERHLNIRPAFVL
                     VPTAMESVANQVIRSSSVKGADINAGIINPVKDFATVIAEPRLDDNSQTTFYLAASKG
                     SDTIEVAYLNGVDTPYIDQMEGFSVDGVTTKVRIDAGVAPVDHRGLVKCTA"
     gene            25988..26311
                     /locus_tag="ACHURS_001143"
     CDS             25988..26311
                     /locus_tag="ACHURS_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HFR1236930.1"
                     /translation="MAKNFVEEGKTVAIVASAAISSGDLVQVGDVFAVALTDIPQGET
                     GDGMTEGVFMLPKLKTDDMKTGKKVYLKSGKVQLTNSGSDPLVGVVWADAGTSAEEVP
                     VKLNV"
     gene            26304..26579
                     /locus_tag="ACHURS_001144"
     CDS             26304..26579
                     /locus_tag="ACHURS_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HFR1236931.1"
                     /translation="MSDPFSRLAARMDAITVRKMGKTASINDVDMTVIPGETLAELNA
                     LSGPAVSLVVFSSGYRPRRGDRVVYDGQQWTVTRHERFNGKPMIFIE"
     gene            26591..27181
                     /locus_tag="ACHURS_001145"
     CDS             26591..27181
                     /locus_tag="ACHURS_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1236932.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRRLPEARFATGHDHQGRPRKNRLPVEVVKIPLSG
                     PLTQAFEDARDRIIAAEMPKQLGYALKQQLRLWLTR"
     gene            27178..27579
                     /locus_tag="ACHURS_001146"
     CDS             27178..27579
                     /locus_tag="ACHURS_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFR1236933.1"
                     /translation="MNRHTQIRQVVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDIPALSGLID
                     TLIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            27590..28333
                     /locus_tag="ACHURS_001147"
     CDS             27590..28333
                     /locus_tag="ACHURS_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1236934.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASASVNVGATTTLTFTVKPDGASDKAISVHSTDPQTATVTLNGLVATVKG
                     VKQGSVSIVGMTSDGDFVAVATVAVSAAG"
     gene            28394..28780
                     /locus_tag="ACHURS_001148"
     CDS             28394..28780
                     /locus_tag="ACHURS_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFR1236935.1"
                     /translation="MFLKQGTFNYEKQSVVLSELSGLQRIEYLAFVQQRTAKFDAEEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASVITTWSYDALGAGAEM
                     VLSLSGMGAIENAGDLEHEVLTPEKS"
     gene            28789..29118
                     /locus_tag="ACHURS_001149"
     CDS             28789..29118
                     /locus_tag="ACHURS_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFR1236936.1"
                     /translation="MQFVMRLAREFRRADWRRMLSEMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRVVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            29090..32164
                     /locus_tag="ACHURS_001150"
     CDS             29090..32164
                     /locus_tag="ACHURS_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR1236937.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASVHAQTVEKSAQAHERMAREVEQTRQRMEALSQK
                     MREEQAQAMALAEAQDKAAAAFYRQIDSVKQASAGLQELQRIQQQIRQARNSGGIGQQ
                     DYLALISEVTAKTRVLTQAEAEATRQKVAFIRQLKEQATRQNLSSSELLRAKAAQLGV
                     SSAAEVYIRKMEQAGKATHSLGLKSAAARQEIGVLIGELARGNLGALRGSGITLANRA
                     GWIDTLMSPKGMMLGGVIGGIAAAVYGLGKAWYDGQKEGEEFNRQLSLTGHYAGVTAG
                     QLWTLSRAISGNGITQHAAAGALAQVVGSGAFRGNDIGMVARAAAQMERSVGQSVSDT
                     ISQFKRLKDDPVNAAKALDNELHFLTATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     RRTADIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQIATLQEKVSRAKRLP
                     WTASSSQVEYDQQRLNDLQEKKRQKDLQDAKEQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKALASGKKKTRETRNDEATRLLL
                     QYSQQQAQVEGQIAAARQSAGIATDRMTEAHKQLLALQQRISDLDGKKLTADEKSVLA
                     RKDELIQALTLLDVKQQELQKQTALNDLKKKTIQLTSQLAEEERAQRQQHDLDIATVG
                     MGDQQRQRYQVQLSLRQKYQQQLEQLRRDSEQKGTYNTDDYRKAEQALTESLNRQLNE
                     NRRYWQQLEIAQGNWKNGAMRAFQNFTADADNAAGTAEQMLTAAFNSAGNALATFCTT
                     GKLNFKSFTASLLSDLAKIMAQMSMMQAVKGIGSAFGWGSAATASVTPNADGGVYQSA
                     DLSRYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADIGGSGMAMF
                     APQYNIEINNDGTNGQIGPAALKVVYDLGKKAAADFMQQQSRDGGLFSGGGR"
     gene            32161..32490
                     /locus_tag="ACHURS_001151"
     CDS             32161..32490
                     /locus_tag="ACHURS_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1236938.1"
                     /translation="MKTFRWKVKPGMDVASAPSVIKVRFGDGYSQRAPAGLNADLKTY
                     SVTLSVPREEATALESFLAEHGGWKAFLWTPPYGYRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            32490..33188
                     /locus_tag="ACHURS_001152"
     CDS             32490..33188
                     /locus_tag="ACHURS_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFR1236939.1"
                     /translation="MQNIRQETLNECTRAEQSASVVLWEIDLTEVGGDRYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFEMNGKGASARPTLKVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYSGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            33193..33936
                     /locus_tag="ACHURS_001153"
     CDS             33193..33936
                     /locus_tag="ACHURS_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR1236940.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     EYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIYK
                     FRCVPHLTGRCFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRNGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGIYNDLVAASTFV"
     gene            33873..34475
                     /locus_tag="ACHURS_001154"
     CDS             33873..34475
                     /locus_tag="ACHURS_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFR1236941.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLNE
                     GWYQVRISGRDTGENELSARLNEPLANGAVIHIVPRLAGAKSGGIFQAVLGAALIATA
                     IWMPGLSIVASNLMFSLGAGMVLGGVAQMLAPKAKTPKVSATDNGKQNTYFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVASQEISTADEGDGGQVVVIGR"
     gene            34548..34886
                     /locus_tag="ACHURS_001155"
     CDS             34548..34886
                     /locus_tag="ACHURS_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001332187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1236942.1"
                     /translation="MNKTILFCTIIALTGCKSLDYVKSGKPVMEGNSLKNIDELSGCI
                     SRQWAGNGTPITSIPIENGVSLLVPQAMGGYDVVLDIKKAGNGSSFTLYERVPALTPK
                     IFADSVNACK"
BASE COUNT         8671 a         8022 c         9618 g         8652 t
ORIGIN      
        1 ttttgaaaaa caattggtta tgtgttttgt gggagcctat tgggaaccta tcaaaagatt
       61 ttttgtcata acagtgtgag atgcaaaaca ctgtgatggt cgctttgttg acatcgcaac
      121 ttctgcaggt ataattcgcc accaatttgt aacctgtgta tatgtacagt catttgctgg
      181 ttataaacac agtgaattgc tcttgcaatc ttccaaaact tttttgttcg tgttaggttg
      241 agataacagt gatgatactg tttgcacatt cagtgtaatg aggagggtgg ttatgtacag
      301 tccggtacaa atagccaata aattcataac gttgggaaac caacatcata atcctctgac
      361 ccacatgcaa cttcagaagc ttacttatat tgcccatggt tactatttag cattaacagg
      421 taagcctttg ctcaatgaat gtgtctccgc gtggaagtac ggtccagtta ttcctggaat
      481 gtacgatgct ttcaaagact atgggaataa acctgttacg aatgtagcag tagctccttt
      541 tggtggcatc gttactatgg atccgcaagc agagagcatt ataggggctg tttataagtt
      601 ttacggctcg aaaaatggaa ttgagttatc aactctaact catatgccag gtacgccttg
      661 gtcacaagct tataatggta ttggctcgtc gatcatctca aatgatgcga tcaaggctta
      721 ttatcatgat ttattgaata accgacagca atgtcagggc ctctgaaaaa gtaatattca
      781 gggatgacat gtctcataat tcagatatct ataaactgat cggagcggcc gcaggtgttg
      841 aaaatggtcg ctctgaagca tccttaaact ctactgacag ccaagagcag gcttttgaat
      901 ccgcatttga gccgtctaat catgagcgcg atgatgattc ttcttcagaa aataaggcta
      961 ttctggaggc agaagagttt ggttctaata ctggtgcatt gcatgagttt atgcagcaga
     1021 acagaatgga cagtcttcaa gctcagcttg atatgctcaa gtcacaagta cgggataaga
     1081 tagctgacgc aactggtaaa gaaatagaca atgagcttcg gacaaaaatg gcctcattca
     1141 cggtttggtt tatgtcgtgt tggtgcttat ttgtagccgg aatgtttgta tcatttctta
     1201 ttgctcatga aggaaagccg ccagttgaag caatcgttgc attactggga acaagtacaa
     1261 ttagtattgt tggtttggtt ggcttcgttg ttagtggatt gttcaaatct agaaaagatg
     1321 gcgataaaga aaaataaccc gtatatacac gggttattca tggtcttagt ttagttatat
     1381 ttttgtttat ccgttctgac cttttcccac tcagctcgtc cttcttctcg tcttttgtct
     1441 atgtattccg caagatcctg aatattgatg caacgttttg ctttttgtga tgtgccgatg
     1501 cgatatgttg gaacgggcaa cttacaagcg tttgcttttg cttctgccgt ggctggactc
     1561 atgccaaagt acttttggct aactgctgag agttcaatgt taggggtatt gaattcagcc
     1621 atcagtaaaa acaaggtgtt cataattttc tccatcaaaa ccggctgcac ccgggaaaat
     1681 cataattctg tgctggtggc aggaattaat ttctgccaga tagcggaaac atattttgcc
     1741 tgatgacggg catcagccag ggcgttgtgc cgttcgccat cgaaaggcat gtccattttg
     1801 gggtcgaatc cgatggaacg cccaagcgta acgatcgtgc gtacatcgtg gtcattccag
     1861 tacgcccacg ggcagatttg tcctgctcgc tcgtaagctc cacgtaaaat tacgttgtcg
     1921 aaggtggccc cgttacccca gacttttaaa tatttcgtat tgtctgcgtg ccggttaatg
     1981 aaatggttta gttctgagag agcatcgctg atcgacaaag tatcatcaat acagattgca
     2041 gctcgcgctt cagggctttg tttcaaccac cacaggatgg tatcgccgtc aggtgtagct
     2101 ccttgcttca tagcactgtc taggctgaca accgcataga attcttgtcc gatgtctccg
     2161 gtttctggag tgaagaacac cgcgccaatg gaaacgatcg gtgcatcctt atttttcccc
     2221 atcgtctcaa ggtcgatcat taagttgttc atcacttcac ctcctgcggc ggttccggta
     2281 gcggcatcca gtgagttgct tgctcaatac cattacccgg cttaatcgtt gcatctccgc
     2341 gccgaaaggt gcttccggta tagcgtgcgg cgcatattag cggttcaacc agagagctat
     2401 cgaaattcac cgaaataagc acgttctggc ccttttcagg cattcgatca ctacagctta
     2461 tccaactatc cggagttccc ggagagttgc catttacatc gaagtttggc tctgcgtcct
     2521 gaaccaggag gatgtaacca ttcttggctg tatcaagttc taacgcctcg gtgacggtgc
     2581 cgaaatagcg attacctaaa tcagcatcac aagtgcttac atcaatggaa acttccatgc
     2641 cttcgattaa ttctggcaag ttgtaagttt ggcttacagg ttggctaccc tgaagcatgg
     2701 cggcgcggtg acaccagata atccagccaa gcgccatatc ccatgccatg tattctctat
     2761 cgccattttt tgccctacgg cgatctacag attccccgaa acgcttctcc ataaataatt
     2821 cataggctgc tcgttcatcc gatactgctg ccagcgatgc cagtgcaatt ttaaatgcgg
     2881 taagtatgtt gttaacctga cctatttcga atgctatttc actacataca aacgatttat
     2941 cgtctattat cgactcaatt ccggtaatcg tgttctgtaa ccattctttg gtaagagtat
     3001 tcataactat ttcactttaa tctcaatatt tcgcagcttt agctctactg gcaggtctga
     3061 ctttcctgtt aatgctaatg cgagattttc aggagtaatg agagcagtta ttgttttccc
     3121 cctcgccaga cgaataatca ttcgtatctc gcaatcgtca catgctcccg gtcgaacaat
     3181 tgatatttgt ccgttcatct cactctcctt tgatgcgaat gcctgcggcg cgtgacacat
     3241 taacttccac gatgcgcaca tttggtttgt acatctcaat cgctgtcagc cagtcagcgc
     3301 cggtcatatg cttttctgca tcgccattag tccattgaac cggcacacca atagctttca
     3361 tcgcaatttc tatttccccg gcaatggcgc ttttcccgca accagtaaaa ccagagacaa
     3421 cgacaagaac ttcgcctttg gctggtttta tttcccgtgc ttccagttca gcaatacgct
     3481 tactcccatc cgagataaca ccttcgtaat actcacgctg ctcgttgagt tttgattttg
     3541 ctgcctccag ttcgtccagt agtgcaatca catcagggtc actgtcatca actactgtta
     3601 cgcgtgattt ttcataatgc tcgtcggcaa gtgtgcgacc aattttgaaa tctccatcac
     3661 caccataacc ggagcaggca taagtgacat gtgctccaga tatacgctgt attgacattt
     3721 cctcgccaca aacagagcat tcaggtactg gcttaggtga ataacgttcc cgtagcgcct
     3781 gataatcaat ctcgctcact ggttgccctc cttcataaaa ataatccagt gggtcttgtc
     3841 accctttcct gttcgttgac cgataacagg ctttctgtcg gtcagtgcca atatctggcg
     3901 aacaggtatt tgcgtttcat tccatttaaa aatcagaacg ccgtatggcc acaacacacg
     3961 aaaggcttct ttgaatccct gccgtaaatc atcacgccag gtatctttat tcagccgtcc
     4021 atatttcttt cccatccaag cgttatcacc aacacgctca agatgcggag ggtcgaatac
     4081 aaccatcgaa aaggatgcgt ctgcaaatgg tagtgcacga aaatcagcta tcaggtcagg
     4141 actgataatc aggcgtcgtc catcacacaa tgtgtgctct tcctttctga tatcactaaa
     4201 tatcgcccgg tcgtcattct tatcgaacca gaacatgcga ctgccacagc acatgtcgag
     4261 gattgctgca tgtccagtca ctggctgcct cctttgcgaa gccgttccgc ccattcttca
     4321 agggatttct ctgcatattc accggacagg ccatcaatcg gatgcgattc attagctaac
     4381 tcttcttttg ctgacagaat catgcgtgta acgtcgaaaa cttcagccaa aggcttattg
     4441 ataaatccgt gattgaaagc agcagcaaga cggcttgcgg tatagttaat accctcgttg
     4501 cgagcctcag cacgtacttc atcgaattta cgcaccagat actcagcatt tgtttcattc
     4561 actttcagat ctcgcggtac acatttcccg cgaagaaacc cttccatttc gaaaacattc
     4621 atgcgcattt gcgtaactcc gataactcgt taaaacgttc cataaacatc ccataggcat
     4681 ggcctggcga cagtggaata actttgaaca tctctgttgc cgggatacct tccagaacag
     4741 gccagaaaga gccatcatca agcccgagat cacggcgttc ggttgccagc ataatgagat
     4801 cggcatattt cactggcgtg ctcataacag gaggtaaccc gtatttctca cggattacgg
     4861 cgtctatttt ttcttccatc cgtttatagt caggaagaag tcgtttcagt ggcgcgggga
     4921 tgtcctggca atatgcttct gttgcatcat gcattaacgc ttcaaaagca aattcctgcg
     4981 gcaccagctg gctgcaaagc accgcatgct gggcgacact gtagaagtgt gaaagatgtc
     5041 ctgcaaagcg acagatattt gaaagggaaa ctgcgatatc gttaatcacg atgtcgtctt
     5101 tatttatctt gtcataataa aaatgcttcc cggaaaaagt tttaataaat gacatttcgt
     5161 tctccacttt atatgcgctg caccgcgctg aattctgcta aaaggaagct cttaccatcc
     5221 ggtgattatt gagttaatta cgtttccata aatgcccccg caggggcatt tgcagtaatg
     5281 aaatcaggcg gtgaaagtac caataaaggt ttctactttg ctgtctttga atttctcaac
     5341 aagcagatca cgaaattcgt tagccatttc ttcctgcatc gcttccagct gaataatgcg
     5401 cagaaccagt acaggacgat cgccagtgat aatgctgagg cgtaatttaa acggacgttc
     5461 tttcaggcct tcaaacggaa cgcatttaaa ttcaaatgcc actggcataa tgtctttggt
     5521 tttcgcttcg acagactcca tcagagagcg tttgccgctg aagtcattgt cttcaaaatc
     5581 agcggtctgg tttgcttcaa ttgtgatttt acggactgcc gcagccgctt tggttgcctg
     5641 aatggtgtca ccattagcat caaagcccac aaggtagtcg gcccagtctt caatccattc
     5701 tgccagtgac ttctgggagt tacgctcgcc attaacagac aacagagcag aaaacggtgc
     5761 tgtctttttc agtttgaggg tggcggtgtt atctgcgtga ccgggttcat caatagtacc
     5821 caggttaagc acactgacgg ctcgcatatt atcggcatcg ataaagcagc gggtgccttc
     5881 atctgcaaga tctttagaat aacgggtaaa gtcatcgatg ctggcagtgg aaagcgcacc
     5941 acggaaacgg aagcgattta aattaaattt ttccagatca tgaatgcgga aattctcagg
     6001 caatgccaca gcatcggcac caatcttact gataatttca ttaacaccct gagcagaaat
     6061 aagggcatgg atttgattaa ttgcggttgc gtctaagttc tgagacataa taagtcctca
     6121 ctatataaag atattcagtg atgagataaa taatcagtta attaagaacg atattaatga
     6181 cctgctgcgc ggagttttcc gtcaggttca ccggcaagag tcagtaattg tccctggtct
     6241 tcctgcagaa tagtcaggcg accaccgcga ttgacataca tcggcgtttc ggtggtgtct
     6301 tcttcggaaa ttttcccgcg gttagtcggg cgaacatatg agagtttgtg ttttattttc
     6361 acacggttct catcaaacgg ttcgatttcc aggttgagcg agaccttacc tttggttttc
     6421 gtgttcatca caccggaagc gacttcactg agaacagcgc cgattttggt ttcaaatacg
     6481 ccgccgtcca gctccccgat aaatgcctgc acatcagtac tgcgttcgct agccattttg
     6541 ctgctcctca tcatatcgac cctgcaaggt cggttggttt ctccacaaaa cagagaagaa
     6601 cacctgcggt gactgccgcc cggatggatt gggttatgag cccgtcgtcc ggtgatgctc
     6661 ttctctgttt tgtaaaaaga gcggtaccag ccggaagcaa gtgtacaaac tggtaccgcc
     6721 aaagcagtgg ctgttgtggt gaccggtgct gatctccggc ttgcggttat ttcagactct
     6781 cacgggcgtt taattgcccc gccgaacagc tcttttccgc aatagctgca atgtctttcg
     6841 cgcatcagcc tgcgcattca ccacaacgct gagagcactt agccagttac ggcaccacac
     6901 tttgtcgcgg ctccataaat gccctcatcg ttgccccctg gtctcttccc aggcgtcaaa
     6961 ccgaatcgcc acgctggtta ggcgtcttat cagcatcatc attgacttgc acattccggc
     7021 tacctggttt gtttgcccga gcaaggagtg gattgtcccc tttaacgtcc ccagaccgct
     7081 aacgacgcat gtgccatacg ccgtgttaca accaaatttt gtttaatctt gcctgtgctg
     7141 tgtttctttt agatacatta tgtatctcaa gggtacattg tcaagtataa aaaaacctgc
     7201 cgaagcaggt tataaatatt gattaggcct ttattttgta tcttcttggt tttcctgaga
     7261 aaattactgt accaattata gagcaattac cgttgatctt aatgtaaggt tcaggccagt
     7321 ttgggtttaa tgctttgagg taacgctgtg ttccatcttc tatcaaccgc ttaaaggtgg
     7381 tttcgcctgt atcgtgcatc aatgcaataa cgtcgtcacc gtggcaggca gggacttcag
     7441 gatctacaaa aatcatgtct cccgggcggt actcatcaat cattgaatca ccaatcaccc
     7501 gcaagatata agtcatttcg ccacagggta cagggcaggg ataagtttct gctgtgctca
     7561 aatcaacctc agaatagcca acttctttcc atgctccggc ctgtacccat gatatgacag
     7621 ggactaacgt tatttgtttg ttagtaattg aaacatcagg ttttttggtg atgtttgttg
     7681 tctggtgttc ttgatcaagc catccgacag gcaggtcgaa acatttttcg atgtgccgtg
     7741 ccatgctgtc accgatattt ttagtagcac catctcccat aaacctgctg gtctgggttg
     7801 gctcgcgatc aatcatggtg gcaaaggaag aattcccgcc aacaccatct ctcagttttc
     7861 tggcgttaga ccgccggatg tcatggactg ttttcataac gaaattaaaa cctttgtacc
     7921 gatagggtac aagtatcttg aaggttcatc ttaatcatgt aatatgtata tcggaggtac
     7981 atattgtatg aaagcgtatt gggactcttt aaccaaagaa cagcagggcg agttggccgg
     8041 aaaagttggc tcaacaccag gctacttacg gctggttttc aatggttata aaaaagccag
     8101 ttttgtgctg gctaaaaaac ttgagcaatg cacgtcaggt gcaattacga aatctgactt
     8161 aagaccggat atctatccga aagattaaca gaacaccttc aatttttaac cacagaacga
     8221 tgaggctaac cgtgggtaag catcactgga aagtagaaaa acagcctgag tggtacgtga
     8281 aagctgtcag aaaaactatc gcggcgttgc cgggaggtta cgctgaagct gctgagtggc
     8341 tggatgtaac agagaacgct ttattcaacc gccttcgtgc agatggcgat cagattttcc
     8401 cgctgggatg ggcaatgatt ttacagcgcg cggctggcac tcactacatt gcggatgctg
     8461 tcgcacagtc tgctggtggg gtgtttgtat cgcttcctga acttgaggaa gtagagaacg
     8521 ccgatataaa ccagcgcctg ctggaagtca tcgaacagat cgggagttac tcaaagcaga
     8581 ttcgttcggc aatcgaagat ggggtagtgg agccacacga gcagacagca attaatgatg
     8641 agttgtatct gtcaatttcg aagctccagg agcatgcagc actggtctac aaaatctttt
     8701 gcgctccaga aaagagtgac gcccgcgagt gtgcagctcc gggcgtcgtg gcgttttgtg
     8761 tctgtggaga aactaacgca tgaacagttt aacggcaaat aaccgtttgt cgcaacagct
     8821 ggtggtcagc gtcgctgaac acctgttgtt acggcatgaa tgcagattac caaatcacct
     8881 ggctgtaagt aaccacagag aactttacct gactgtgggg ggcgagttgt gcaggaactt
     8941 aaccgctggt ttcgtgacgg aagagggctt tatgtccatg ttattcgttg ggagccagaa
     9001 acacagcgcg ttatctatct tcgcaaagac tacccgcatg agtgctttag tcctttgtgg
     9061 aaattcaggc gtgattttgt tgagtgtgaa ggaccaccag cacattgatt ctgccattcc
     9121 gggacgttac actgttcagg caccttataa agcgggtgtc gggattggcg tcctggaatt
     9181 caatatagag cataaccgcg ctcatgcggt tttttcgtat catgagcatt gctacgtcca
     9241 aattatggtg gggcgtgcag gggcatcgca agatgcgccg ggttctatgt tgaccggtta
     9301 cgccaaccct gtacgtctca ccacctctgt gattggcgtc ccatgtggtg agttctttga
     9361 attcaacata ggggctgtca ccatgactac tctcccaacc ctcgctcgtc tgaatgatga
     9421 agacttacat aaactcagtt atgtaacaac tgcactacgt gctctgcgca aggtaactct
     9481 ttcggatccg caggcgcatc aggttctggt agaaacactt cttaacttgc aggctgaacg
     9541 tattcgtttg gcggataagg ctaattttca tattcaccgt ctcctgaata tcagcggagg
     9601 gcatcgtcat gcttaatccg ttgatcctca atatttgccg tttgcttcag cgtaaaaaaa
     9661 catcaattcc tacagttggg cagtggtaca ccacgcctgc agggcatgtt ctacgtgtta
     9721 gcctggttga ccgtgaatgt cagaaggtga tttgtgaacc gctgggccgt aattaccgcg
     9781 tcagtatgcc gcttataacc tttcgctccg gaaaaaacat gaagcatctc ggaggtgcag
     9841 catgagtatg gagctgatgg ttaaagcgat gaaaattcga gtgggaaatc cattgcgaaa
     9901 actggttctg attaagctgg ctgataatgc cagcgatcag ggtgagtgct ggcccagcta
     9961 ccagcatatt gctgaccagt gcgagattag caaacgttct gtgatgaatc atattgcggc
    10021 cctttgtgag tccgggctgg taaaaaaagt cacccggaaa ggtgaaaaag gtaactcaag
    10081 taatatctat ctccttcatc tggatggtgc aggagattca ctagggggta gtgcaaataa
    10141 ttcactatct ggtgcagcaa attcaccagg tagtgcagga gttgcaccag ggggtagtgc
    10201 aggagattca cccagaacca gtcactcttt tgaaccagtc aaagaaccag tcaatgaacc
    10261 aatagctgtt ggtgcatctg ctgatgagtc tgtgcgagtt cgttcaaacc gaccggaata
    10321 ctctccggag tttgagcagg catggctggc atatcccaaa cgtgctggtg gcaattcaaa
    10381 atctgcagcc ttcaaagcct ggaaagcccg tttgaatgag ggggtaaacc ccgaaaccat
    10441 gctggaaggt gtgaaacgct acgcgggctg ggtatctgcg atgggtaaca gcggcacaca
    10501 atttgtgaaa caggctgtca cgttctttgg tccggatcgt catttcgaag aatcctggga
    10561 agttcctgcg gtatctgcag ctagacgtga ggacccgtac ttcaaagcca gttacgacaa
    10621 cgtggactac agccagatcc cggcaggatt cagagggtga tcatgagtct gttaaatgac
    10681 gttcagaaat tcattgaagc ccatccgggg tgtatttccg gagacattgc ggatgctttt
    10741 gcaggttact cacggcagcg cgttctgcag tcagcaagca agttacgtca gagtgggcgt
    10801 gtggctcacc gttgtgaagg agatacacgc agacatttcc cacgcctgac tgagagagcg
    10861 caggagccgg aaccacaacc agttcgtgaa accagacctg tgcgcaattt ctatgtcggc
    10921 actaacgatc cccgggtgat tttgtgcctg acccgccagg cggaagaact ggagtccagg
    10981 ggcttatacc gtcgtgctgc aacggtgtgg atggcggcat tccgtgaaag ccactcccag
    11041 ccagaacgaa acaattttct ggcgcgtcgt gagcagtgtt tacggaaaag cagcaagcgc
    11101 gctgtatcgg gtgatgagtg gtatctgtca gggaattacg tgggggctta atgagtaata
    11161 aatattgcca ggagctggtg gaactgcgga acaaaccagc ccatgaactg aaggaagtgg
    11221 gcgatcagtg gcgcacgccg gacaacattt tctggggaat taacaccctg tttggcccgt
    11281 ttgttctgga tctgttcacc gacggtgata acgccaaatg tgccgcgtat tacacggcgg
    11341 aagacaatgc gctggcgcat gactggtcag aacgtcttgc ggagcttaaa ggtgctgcct
    11401 ttggtaatcc cccatacagc cgcgccagtc agcatgaggg gcaatacatc actggcatgc
    11461 gttacatcat gaagcatgcc agttccatgc gtgataaagg cgggcgctat gttttcctga
    11521 tcaaagctgc caccagcgaa gtgtggtggc cggaagacgc agaccatatt gcttttattc
    11581 gcgggcgtat tggttttgaa ctgcctgcct ggtttatccc gaaggatgag aagcagatgc
    11641 cgacaggcgc tttcttcgct ggtgctattg ctgttttcga caagacctgg aagggaccgg
    11701 caatcagcta catcgggcgc gatgaacttg aggcatgtgg tgaggccttt ctggcgcagg
    11761 ttcgccagca ggcggaaaaa ctggtcaggg agatggcggc atgacgacgt taactcaatg
    11821 ccagcagcag gtgctggata tgctgatttc ttaccagaaa gaacgtggct tcccgccaac
    11881 caatcaggag gtggcaacca tgctgggata ccgttcagtg aatgcagcgg tggagcatct
    11941 tcgcgcactg gagaaaaaag gcgtcatcac gataaagcgt ggcgtggccc gggggatcac
    12001 gcttcatacc gcggtgaagg acgacgacag cgaagcggtc ggtatcatcc gcgcactgct
    12061 tgccggtgag gagaacgcca ggttgcgtgc agcccactgg ttacatgaga ggggcctgaa
    12121 agtatgaagt tgatccttcc tttcccgccc agcgtgaaca cgtactggcg acaccccaac
    12181 aaaggggcat ttgctggtaa gagcctgata agcgcggcgg gacgcaaatt ccagagcgcg
    12241 gcgtgagccg ccatcattga gcagttacgt cgtctgccga aaccaacgtc ggcacctgct
    12301 tcagtggaga tcgtgttgtt tcctccggat aacaggatcc gcgatctgga caactataac
    12361 aaggcgctgt ttgacgccct gacccacgcg ggggtgtggg aagacgacag tcaggtgaaa
    12421 agaatgctgg tggagtgggg accggttatc ccggaaggga aggtcgagat cactatcagt
    12481 aagtacgaga aaacggcggg tgcagccgcc tgatcaagag gagaaacgaa gtatgaataa
    12541 tctgatggtc attgatggta ttgaagttcg tcgtgatgct tatgggcgtt acagcctgaa
    12601 cgatctgcat cgcgcagcag tagcatctgg tgcaaatgcc agaaccaagg agccgggaaa
    12661 gtttctttcc agccaacaaa ctgttgagct tgttcatgaa ttgaccaaca cccagaattt
    12721 gggtgttgac ccggtgagtg tgattcatgg gggaaatgaa cggggaacgt atgtctgcaa
    12781 ggaactggtg tatgcctatg caatgtggat cagcccgtca ttccatctga aggtgatccg
    12841 tactttcgat atggtaacca gcgcaccgga aaaattatcc ggacaggctg ctgacaagat
    12901 gcaggctggc gtgatcctgc tggactttat gcgccgggag ttaaacctgt ctaactcttc
    12961 agtgcttggt gcctgtcaga aactccagga ggctgttggc ttaccgaatc tggcaccgcg
    13021 ctatgccatt gatgctcctg ctgatgcaca cgatggctca agtcgcccga cactgtcact
    13081 gagcgcactg ctgaaacagt atggtatacg cctgacggct aatcaggcat atcaccagat
    13141 ggtgaaactg gggatcgtcg agcagcgcga acgatacagc cgtaccgcga ttaacaacat
    13201 caaaaaattc tggtcgctga cagcgaaagg ttgcatgttc ggcaagaaca tcaccagtcc
    13261 cgcaaatccg cgcgagacgc agccgcattt cttcgaatcc cgattccctg agctgttaaa
    13321 gctgctcgat accgttcatt gaggtgaccg tgagagcact actgacccct gaaattgccc
    13381 cgcgtatggg gatcgtattg ttcaggccag gttcagagct gatgcccctg tttatgcagg
    13441 ggcgtgtcct gctggagcct gagccggagc gttattcatc tttcgccagt ggtgccgttc
    13501 cggcggcatc acaaccgctg gcggatgagc ctgccgttcg ggccgtgttc cgcaatgagg
    13561 cagtgatccg tcgtgctggt ggcgtggaat gtcttgaaag ctggttactt cgtgaaaaag
    13621 gctgccagtg gcctcattcc gactggcaca gcgagaacat gaccacaatg cgacacgctc
    13681 cgggtgcaat ccgtctgtgc tggcactgcg ataaccagct gcgcgatcag ttcacggaac
    13741 ggctggaatc aatggcaacg gataactgtg cccgctgggt gttgtctgtt gtgcgtcggg
    13801 atctcggttt tgatgacagt cacgttgtga caatgccgga actgtgctgg tggctgattc
    13861 gtaatgatct ggcggatgcc ttaccggaaa gtgcagcccg taaggcactg agattaccga
    13921 agcctgttgt gccgtctgtt acccgggaaa gtgaccttgt gccttcggtt cctgccacca
    13981 gcatcattca ggataaggcg aaaaaggtgc tggcgctgaa agtggatccg gagtcgccgg
    14041 agtcttttat gttacgccca aaacgtcgcc gctgggttaa tgaaaagtac acgcgctggg
    14101 ttaagacaca gccgtgtgca tgttgtggaa agcccgctga tgatccccac cacctgatag
    14161 gtcacggtca gggtggaatg ggaacaaaag cgcatgacct ttttgtgttg cctttgtgca
    14221 gaaagcatca cgacgagctg catgcggata ccgtggcatt tgaagagaag tatggctccc
    14281 agctggagct gatatttcgt tttatcgatc gtgcgctggc aattggcgtg ctggcctgat
    14341 tttgtggaga aagttgatgc gtgatattca aatggttctt gaacgttggg gggcatgggt
    14401 ggcaaataat cacgaggatg tggaatggtc atctgttgct gcaggtttta agggattaat
    14461 tccttcgaaa gtaaaatccc gcccgcaatg tagcgatgac gatggcctga tcattagctc
    14521 tgcgatgaca gttcttaaga aaaaggaacc gtatcaatac gaattactgg aaatgtatta
    14581 tgtgtatggg gttacattac gggcgttggg ggtaaaactg gggatatcac ttaatcaggt
    14641 tgttatcaga ctgcagaaag ctgaagggtt tattgacggt tgtctggcaa tgttgggggt
    14701 atctttagaa attgattgtt acatatagta ataaattcaa tcaaagtaaa taatcatatt
    14761 ttattataac ctcctgatga tacctgttca ttgggaggtt attatggata aaaatgtaga
    14821 gcatgtatta gttgatgcaa ttgaaaataa gcaatcttta acagtcgttt acttaggagg
    14881 gagccagcct ggaacattaa ggaatatttc tccgattagt ataaatgggg ataaattgcg
    14941 ggcaagatgc catagttctg gagcagtaaa ggttttcaat cttgggaaaa tacagttacc
    15001 cagtgacccc tgcgcggtat ctatgcacta tggagattta gaagttaaag cttatgagac
    15061 gatgcagagc gtaaatgaca actttcatgc cctttatcct gaaggacgat ggggtgttga
    15121 ttttaatgag catcgctttg ctttatttga tttttttaaa aacgggaaac gaaaaaaaac
    15181 ggcatttatg gcaattgagt tcagggaaag agatgaagag aaaataataa caggtgtaac
    15241 aattgatatt ggtatatctg gaacagtgat ttctgagaag tcccgaatcc caaaaagacg
    15301 accatgggta gtggttggtc ccgaacacgg agaatacagt acttattcaa ctttggacaa
    15361 ggctgctaca gcgttttttg agaggctttc gttgatagca tccggcctgg aagataattg
    15421 attttatgtt tggtattcag agttcgccgt gcttaagaaa gtcaagattc taaaaatact
    15481 gaatgagcta cttgtgttat aacaaaaatg ctattagtgt gttaagagtg gttacttcgc
    15541 cacacaactt aaacccgcca ctgagcgggt tttttgtacc tgtaaacttg gtgcagtaca
    15601 gtaaacacgc tggtggtcgt gaatactgac tttttatctt gctggctttt tagacaagag
    15661 ttattggtat gtcatgttaa ccagaaggga aaaagacatg ctaaaacagc aagatatgac
    15721 agaaaccgcc gccgcagtcc ttcatttctt acctgctgac aagtgggtaa cgccacgcat
    15781 gatgacgaga actaccggag taagcgaagc ccggtgccag ttaatactga ctcagttagt
    15841 tctggcgggt ctggcgaagg ataacggcgg atacgggaat aaattcagac gctgccagta
    15901 atggcggttt cctgctgtga aaatgggcgg ctggtgggtg ttggtagcac ctgccagcca
    15961 ttcgctcatg cttactggtc acaagcgaac cacggcccac tgctttagcg caaaagcaga
    16021 gtgagcctac cagagttacg cttactgatc catgaaaaat actgtaaaaa taaacagtgt
    16081 tgatttaatc aacgctgatt gcctgcattt tattcagtcc ctgcctgatg attccattga
    16141 cctgattgtt accgatccgc cgtacttcaa ggtgaaaccc aacggttggg acaatcagtg
    16201 gaaaggggac gaagattacc ttaagtggct ggaccactgt ctggcccagt tctggcgggt
    16261 gttaaaacct gccggaagcc tttacctgtt ctgtgggcat cgcctggcat ctgatattga
    16321 gatcatgatg cgtgaacgtt tcaacgtgct taaccatatc atctgggcga agccgtccgg
    16381 acgttggaat gggtgtaata aagaaagtct gcgtgcatat tttcctgcca cagagcgagt
    16441 tctgtttgct gaacattacc aggggccata tcgcggcaaa agtgacggct atgcggcaaa
    16501 agaaagggaa ctcaaacagc acataatggc accgctgatt tcgtatttca gggatgctcg
    16561 tgccgaactg ggtataacgg caaaacaaat tgccgaagcc acaggtaaga aaaatatggt
    16621 ttcccactgg tttggtgcca gtcagtggca gttgccgaat gaggctgact atcggaagtt
    16681 acaggcactg ttttcccgta tagcggcaga gaagtttcag gaacaacaac tggaacaacc
    16741 acaccaccag ctggtggcat cttatgattc actgaatcgc aaatattctg aattgctgga
    16801 tgagtttaaa tctctccggc gctatttctc cgtatcagtc tccgtgcctt ataccgatgt
    16861 ctggatgcat aaacccgttc agttctaccc ggggaaacat ccgtgtgaga aaccggcgga
    16921 tatgctcagg caaataatca atgccagtag tcgaccaggt gatctggttg ctgatttttt
    16981 tatgggatcc ggttccacaa taaaagcagc aatggcgctg gggcgtcggg ccttaggtgt
    17041 tgagcttgag tcagagcggt ttaaccagac agtgaaagag ataaacgagc tggtggggaa
    17101 ataatctggt ggccacgtca ggtggccttt ttatttccat tacacagcac ccgcatctgc
    17161 gaggtggggt tatgaaatcc atggataagt taacaacggg tgtcgcctat ggcacctcag
    17221 caggtagtgc cgggtactgg tttttacagt tgctcgataa agtcacgccc tcacagtggg
    17281 cggcaatagg tgtgctgggt agtctggtat ttggcttgct gacgtatctg acaaaccttt
    17341 atttcaagat taaagaagac aagcgtaagg ctgcacgggg agagtaattc aatgactcaa
    17401 aactatgaac tgattgtgaa agggatctgc aattttgaga ataaagttac ggtaacttta
    17461 gcgttacggg acaaaaaacg ctttgacggt gaaatttttg acctggacat ctcgctggac
    17521 cgtgttgaag gtgccgcgct ggagttttat gaggcagcag ccagaaggag catcagacag
    17581 gtcttcctgg atgttgctgc cgggttatgt gaaggggatg agcagtcgcc ggaaaagcgc
    17641 cccgtaattt tagatgcgca gaatgtgtgg ataacctaca aaggaaagct accaggaaga
    17701 attactggtt ctctgaagac tcctccggaa tcacaacctt aagtcactga ccggaacaga
    17761 taaacctgtc cgtgggcaga aaccgataaa tcctgataaa tatccatgaa cgcaaaaatc
    17821 agatacggcc tgtcggctgc cgttctggca ctgattgccg tcggtgcgcc cgcgcctgat
    17881 attctcgacc agtttctgga tgaaaaagaa ggtaaccaca caacggcata ccgcgatggg
    17941 tccggcatct ggaccatctg tcggggtgcc acgatggtgg atggaaaacc cgtttttccc
    18001 ggtatgaaac tgtcgaagga aaaatgcgac caggtcaacg ccattgagcg tgataaggcg
    18061 ctggcatggg tggagcgcaa tattaaagta ccactgaccg agccacaaaa agcgggtatc
    18121 gcgtcatttt gtccctataa cattggcccc ggtaagtgtt ttccgtcgac gttttataag
    18181 cggctgaatg ctggtgatcg taaaggtgca tgcgaggcga ttcgctggtg gataaaagat
    18241 ggtgggcgcg attgccgcat acgttcaaat aactgctatg gacaggttat tcgtcgtgac
    18301 caggaaagcg cattagcctg ttgggggata gatcagtgag cagagtcgcc gcgattattt
    18361 atgctctggt tatctgcatc atcgtctgcc tgtcgtgggc ggtcaatcat taccgtgata
    18421 acgccatcgc ctacaaagaa cagcgtgata aaaaagtcag tgagctgaag caggcgaccg
    18481 ccaccattac tgacatgcag caacgccagc gtgctgctga tgtactcgat gctaaataca
    18541 cgaaggagtt ggctgatgcg aaagctgaaa atgatgctct tcggcgcaag cttgataatg
    18601 gtggtcgggt gctcgtcaaa ggaaaatgct ctgtgccatc ctcagccgaa acctccagcg
    18661 cctccggcat gggcaatgat gccaccgtcg aactctctcc agttgctgga cgaaacgttc
    18721 tcggtatccg ggacggaatc atcagagacc agacagcact gagaacgctt caggaatata
    18781 tcaggacgca atgccttcga tgatagcgat aattttactc atcatccttc acatttggct
    18841 ctgtagacag ggtggtgatc acttctggag tgaatccaga ttaaacatct cattgctgat
    18901 gcttgaagtt gagcatctgg cgcgcggtaa ggggctgcgt tgaaataaga gccagtcatt
    18961 aaaaatacct ggatttagcc tcgcattcgc ggggcttttt attgccatta caaaagccac
    19021 tccctacaga gtggctttga taatggctta taccctacac gggataactt aactgatatc
    19081 ccttttaacg gataaaggta ttcaagcctg acacatcatg cgctgtatcg tcgccgtatt
    19141 cccgtattaa cagagaccgt agcccgacgg ggaactcctt ctgcgagagt gtgcgggaat
    19201 aatcaaaaac gatgcacacc gggtttttac cgcgtttatg gttcgcgggt ttgtccctca
    19261 tgctcgccag tcctgtgcgg gggtggaaga aacaggatac tcacacagat tcttgtgggc
    19321 acgatgctat tcctttctgg attatcccga tgccattcat gcaaggcgct gtatcagacg
    19381 ttcgtcatgg ctgtcaggct gacgggtcct cccggtgggg tggcctgcca cggggcggga
    19441 gcgtcgcgga aaaaggctag tttttgcatt tttatcggcc accatcatcc ttgcatctta
    19501 ttgattatta atggttattt gttttttgta tgtcgaattg agtgtttttt gttcgacatc
    19561 gaacgcgttt tcttaaagtt gttcgcacga tgcatgttta aagctctctg gaggaaatat
    19621 ggatcatgag ttgaaaaacc tggtgctgaa tattaatcaa ctggcggctt tatctggtct
    19681 gcaccgccag actgtcgtgg caagactgaa aaacattcgt cccgctggtg gacatgacaa
    19741 actcaagcta taccggttga ccgatattct gactgaattt atggggttac caccgccggt
    19801 tgctgagggc gaaatggatc cacatgaacg caaagcctgg tatcagtctg aacgtgagcg
    19861 tcttaagttc gaacaggaaa cggcacaact cattccggcc agtgatgtca gacgggagtt
    19921 tgccatctgg gcaaaagcgg tcgtgcaggt gctggagaca ttaccggata ttctggaacg
    19981 tgactgcggc ctgcagcctg ccgctgtgag ccgtgttcag tccattattg atgatctgcg
    20041 cgatcagata gccctgcggg tgaccgaagc aggtgcggat gatgaggagg aattacagca
    20101 ggaggagtaa tgctgaatca ggaaaccgca aaggcagcac gaaccgattc aggttatatc
    20161 cttcgcgcac cgagacgaat gctggttgct gatgccgttg ctcagtatat gcgggtgccc
    20221 atgggggccg ggaattcagt cccgtgggat ccgctggtgg caccgtatgt tattgagccg
    20281 atgaactgcc tggcctcgcg tgaatacgac gcagtgatat ttgttggccc ggcacgaact
    20341 ggcaagacta tcggcctgat tgacggctgg gtgatttaca acgtgatttg cgatcctgct
    20401 gatatgctga tcattcagat gacggaggaa aaagcccgcg aacactccaa aaaacgactt
    20461 gccagaacgt ttcgcgtcag cccggaagtg gtcagtcgcc tgagtccgaa caaaaatgac
    20521 aacaacgttt atgacagaac attccttgct ggcaactacc tgaaaatcgg ctggccgtca
    20581 gtcaatatca tgtcctcatc agattataaa tgcgtggcgc tgacggatta tgaccgtttt
    20641 ccggaagata ttgatggcga gggggatgcc ttctctcttg cctcaaaacg taccaccaca
    20701 tttatgtcca gtggtatgac gctagtggag agttcccccg gcagggatgt gaaggatgtg
    20761 aaatggcgac ggacttcacc gcatgaggct ccaccaacca cggggatact gtcgctctat
    20821 aaccgtggcg atcgccgtcg ctggtactgg ccctgtccac actgtggtga gtattttcag
    20881 ccctgcggcg atgtggttgc tggtttccgt gatattgccg atcccgtgct ggcaagtgag
    20941 gcggcttata ttcagtgtcc ttcctgttca ggacggatta tgcctgaaca aaaacgtgag
    21001 ctgaacggac gtggggtctg gttgcgggat ggtgaatcca tcaatgcgga tggcagtcgt
    21061 tatggtgatc cccgacgctc acgtattgcg tcattctgga tggagggtcc ggcagctgct
    21121 taccagacac tctcgcaact cgtttacaaa ctgcttactg cagaacagga atacgagaca
    21181 accggaagtg aagaaacact caagacggtt atcaataccg actggggatt accttatctt
    21241 ccccgtgcca gcatggagca acgaaaaagt gaactgcttg agcagcgggc agagccagtt
    21301 ccttcccgca gtgtgccgga tggcgttaat ttcctggtgg cgacagttga tgtgcaggcg
    21361 ggacgtcatc gccgttttgt ggttcaggta acgggctatg gcagccgtgg cgaacgctgg
    21421 attattgatc gttacaacat cacgcagtca ttgcgcggtg acagcgacgg ggagagccag
    21481 cgaattgatc cggccagcta tccggaagac tgggatgtcc tgctgacgga tgtttttcat
    21541 aaaagctggc cgctggcctc cgatccttct caacaaatgc gactgatggc aatggcggtg
    21601 gactccggcg gtgaagacgg ggtcactgat aatgcctata aattctggcg tcgttgccgt
    21661 cgtgatggcc ttggtaaacg tatttacctg tttaagggcg acagcatccg gcgcgcaaaa
    21721 ctgatcaccc gtacattccc tgataacacc ggacgaacgg gccgacgggc gcaggccgca
    21781 ggtgatgtgc cgctctggct tcttcagacg gatgccctga aagaccgggt gaataacgcg
    21841 ttatggcgtg actctccagg tcccggctat gtgcatttcc ctgactggct ggggagctgg
    21901 ttttacgacg aactgacgta tgaagagcgg agtagtgacg ggaaatggag taagccgggt
    21961 cgcggtgcca acgaagcttt tgacctgatg gtgtatgccg aggctctggt cattctgcat
    22021 ggatacgaaa agatccgctg gccggatgca ccggagtggg cgagccggga aacctggctg
    22081 gagtgtgtcc cggacagtac cgaaccgtca ctctcaccgg aaccggtatc cacgcctgtt
    22141 aaaaaacaaa aacggaagaa aacagtaact gacgatgtta acccctggct gacttccgga
    22201 ggatggttat gaaccagaat gatatcgaag ccatgattca gcgttatacg gaagctgaaa
    22261 tggcggtgct ggacggaaaa tccgtcacct ttaatggtca gcagatgacc atggaaaact
    22321 tatctgagat ccggcaggga cggcaggagt gggagcgccg ccttgcggct ctgattacac
    22381 gacgacgggg gcatcccggg taccggctgg cgaggttctg atggcaattc ttgatgatgt
    22441 gattggcgtt ttttcaccag gatggaaagc ggcaaggctg cgttcccgtg cggtgatcca
    22501 ggcttatgag gccgtaaaaa cgacacggac acacaaagcc cggcgggaaa accgaactgc
    22561 cgaccagtta agccagtacg gggccgtgtc gttacgtgag caggcccgtt accttgataa
    22621 caaccacgat ctggttattg gtgtatttga caagctggaa gaacgggtgg tggggaaaaa
    22681 cgggattatt gtcgagccac atccggtatt acgcaatggg gccattgccc gtgatctggc
    22741 agcggagata cgcacccgat ggagtgaatg gtctgtcagt ccggaagtca ccgggcagtt
    22801 tacccgtccg atgctggaac gtctgatgct gcgtacctgg ctgcgcgatg gtgaggtgtt
    22861 tgcccagatg gtttccgggc gcataaacag cctgacgcct tctgccggtg ttcatttctg
    22921 gctggaggcg ctcgagccgg actttattcc catgaccagt gatgagagca acaggctgaa
    22981 tcagggcgtg tttgttgatg actgggggcg tcccgaaaaa tatctggtgt ataaaagccg
    23041 tcccgtatcc gggcggcaga tggaaaccaa agaagtggat gcagagcgaa tgctgcatct
    23101 taaatttgtt cgccgtctgc accagatgcg cgggacgtct ttgttgtccg gtgtgctgat
    23161 ccgcctcagt gccctgaaag agtatgaaga ttctgagctg actgcagcaa ggatcgccgc
    23221 tgctctgggg atgtacattc ggaaaggcga cgggcagagc tatgaaccgg atggtaatgg
    23281 cagcaaggag aatgaacgcg agcttaccat tcagccaggc attatttacg acgatctgaa
    23341 acccggcgaa gaaatcggaa tggtgaagtc ggatcgtccc aatcctaacc ttgaaacttt
    23401 tcgtaatggt cagttgcgtg ccgtggcggc gggcagtcgt ctgagttttt ccagtacggc
    23461 acgcaactat aacggcactt acagcgccca gcgtcaggaa ctggttgagt ctactgatgg
    23521 ctacctgatc ctgcaggact ggtttattgg tgccgtcacc cgcccgatgt atcgtgcctg
    23581 gctgaaacag gctgtggcat ccggtgttat caggctaccc cgcgatcttg accgttcttc
    23641 actgtatacc gcggtgtatt ccggaccagt gatgccgtgg attgaccctg ttaaggaggc
    23701 tgaggcctgg aaaatccaga ttcgtggtgg agcggcgaca gaatcagact gggtacgtgc
    23761 tggtggtcgt aatccggatg atgtcaaacg tcggcgcaag gccgaaattg atgaaaaccg
    23821 caagctggat ctggtatttg ataccgatcc ggccagtgat aaaggaggca gcagtgccgc
    23881 aacgaaacga caggagccgc agcacaccga cgaccagtcc gaagaataat tcctggttca
    23941 ggatgcaggc tggtcaccag agtgacgcgg atatttatat ttatgacgag attggtttct
    24001 ggggtgttac agcgaagcag tttatcagtg atctgaatgc actgggcgat atcacccaca
    24061 ttaatctcca tattaattca ccgggtggcg atgtctttga aggcatcgcc atttttaatg
    24121 cgctgaaaac acatggtgcg tccattaccg tttatgtcga cggtgtggcg gcgtcaatgg
    24181 cgtcggtcat tgcgatggtg ggaaacccgg tcattatgcc ggaaaacacc ttcatgatga
    24241 ttcataaacc atttggcttt acgggcggtg atgcggagga catgcgcacc tatgccgacc
    24301 tgctcgataa ggttgaggcg gttctgttac ccgcttatgc acagaaaacc gggaaaacca
    24361 ccgatgaaat tgctgccatg ctggcggatg agacctggat gtccggtgcc gaatgtctgg
    24421 cacatggatt tgctgatcag gtaacgccag ccgttaaggc aatggcatgt attcagtcaa
    24481 aacgtacaga ggaatttaaa aagatgccgg aatccattcg aaacatgatt actccgccac
    24541 gcaacagtgc tccacgcgta caggatgatg aacctgcagc ctccaggacg ccagtgcagg
    24601 cagcagcacc cgtggtggat gaaaacagta tccgtgcgca ggtactggca gagcaaaaag
    24661 cgcgtgtaaa cggtattaat gatctgtttg ccatgtttgg cgggcgttat cagacgttgc
    24721 aggctcagtg tcttgccgat cctgaatgtt cgctggagca ggcccgcgaa aagctgttga
    24781 acgagatggg gcgcgagtcc acgccatcca ataaaaatac cccggctcat atttatgccg
    24841 gtaacggtaa ttttgtgggg gacgggatcc gccaggcgct gatggcgcgt gccggatttg
    24901 aaaaaaccga acgtgataat gtctacaacg ggatgaccct gcgtgaatat gcccgtatgt
    24961 cactgactga acggggtatt ggggtttcca gttataaccc gatgcagatg gtcggtgcgg
    25021 cgttcacaca cagtacgtct gacttcggta atattctgct ggatgttgcg aacaaagcca
    25081 ttctgcaggg ctgggaagat gcccctgaaa cctatgaaca gtggacgcgg aaaggtcagt
    25141 tgtctgattt taaaattgcc catcgtgtgg gtatgggggg cttcagtgct ctgcgtcagg
    25201 tgcgtgaagg ggcggaatat aaatacgtca ccaccggaga taaacaggcc actattgcac
    25261 tggcgaccta tggcgagctg ttcagtatca cccgtcaggc cattatcaat gatgatctga
    25321 atatgctgac cgatgtcccg atgaaactgg gccgtgcggc gaaatccact attgccgatc
    25381 tggtttatgc cattctgacg tctaacccga aaatctccac agataatgta agtctgttcg
    25441 ataaagcgaa acatgcaaac gtactggaga gcgctgcaat ggacgtggca tcgctggata
    25501 aagcccgcca gttgatgcgc gttcagaaag agggggagcg tcatctgaat attcgtcctg
    25561 cgttcgtact ggtaccgacg gcgatggagt ctgttgctaa ccaggtcatt cgctcctcaa
    25621 gtgtcaaggg ggctgacatt aacgccggta ttattaaccc ggtgaaagat tttgcgaccg
    25681 ttattgcaga gcctcgtctt gatgataaca gccagaccac tttctacctg gctgcgtcca
    25741 aaggctccga tacgattgaa gtggcttatc tcaacggtgt ggatacgcca tatattgatc
    25801 agatggaggg cttcagtgtg gatggcgtga caacgaaagt gcgtattgac gccggtgtcg
    25861 cgccagttga tcaccgcggt ctggtgaaat gtacggcgta aacgtcgcag acaacaactc
    25921 tgatggcccg taagggcttt ttttgtacct gaaatcagcc cctgaacggg gctgtgcgga
    25981 gacagttatg gcaaagaatt ttgtagaaga aggaaaaacg gtggcgattg ttgccagtgc
    26041 agccatcagc agcggagatc tggtgcaggt gggtgatgtt tttgcggtgg cgctgaccga
    26101 tattccacag ggtgaaacag gcgacggcat gaccgaaggt gtgtttatgc tgcctaagct
    26161 gaaaacggat gacatgaaaa cgggtaagaa ggtttatctg aagtccggaa aagttcagct
    26221 gactaacagc ggctctgatc cgctggtcgg ggttgtctgg gcagatgccg gaaccagtgc
    26281 agaagaagtg ccggtaaaac tcaatgtctg atcccttttc ccggctggca gcgcgtatgg
    26341 atgcgatcac ggtcagaaag atgggaaaga cagcctcgat taatgatgtc gatatgactg
    26401 tgatcccggg agaaacactg gcagagctga atgctctgtc cggacctgcg gtatctctgg
    26461 tggtgttttc ttcgggatac cgcccacggc gcggggatcg cgttgtttat gacggacaac
    26521 aatggacggt cacacggcat gaacgcttta acggtaagcc aatgatcttt attgagtaaa
    26581 gaggtgtggg atgaaggggc ttgagaatgc catccgcaat ctgaacagcc ttgatacccg
    26641 tatggtgcca caggccagcg catgggcgat aaaccgtgtg gcacagaaag cggtctcggt
    26701 tgccacccgg caggttgccg ggaataccgt tgcgggagat aaccaggtga aagggatccc
    26761 cctgaaactg gtacgtcagc gtgtccgggt gtttaaagcc agtccgtcag gaaaaatgac
    26821 ggccaggatc cgcgttaacc ggggtaatct gcccgccatc aaactgaaca caacacggcg
    26881 gcgtgctggt gaaggactga gagtgggaaa atactttttc cggggggcat ttgttcagca
    26941 actggcgaat ggccgctggc atgttctgag gcgtcttcct gaagcgcgtt ttgcaacagg
    27001 gcatgaccat cagggcaggc caagaaaaaa tcgtcttcct gtggaggtag tgaaaatccc
    27061 gctgtccgga ccgctgacac aggcatttga agatgcccgc gaccgcatca ttgctgcgga
    27121 aatgccgaaa cagctggggt atgcactgaa acaacaactg agattatggc tgacccgatg
    27181 aaccgacata cacaaatccg ccaggtcgta ctggcacgcc ttcgggaaca gtgtggagac
    27241 agcgccacgt tttttgacgg gcttccggca tttattgatg cgcaggaact gcctgccgtg
    27301 gcggtgtggc tgagtgatgc tcagtacacc ggaaaaatga cggatgaaga tgactggcag
    27361 gctgttctgc atattgccgt cttcatccgg gcacaggcac cggattcaga gctggatatg
    27421 tggatggaga gcaccatttt tcctgccctg aatgatatac cggcactttc cggactcatc
    27481 gacaccctga tcccactcgg ttttaactat caacgtgata atgagatggc cacctgggcg
    27541 atggcggaaa tcacgtacca gatcacgtac acgaattaag gaggtggtaa tgaccacacc
    27601 aaatccactg gcaaagacga aaggtgcggg gacgacgttc tggatgtata ccggcaacgg
    27661 cgatgcgttt gcgaatcctt tgtcggacac tgactggctg cgtcttgcga tggtgaagga
    27721 tctgcaacct ggcgaaatga ccgctgatgc agaagatgac acttatctcg atgatgaaga
    27781 tgcagactgg aaaacgacaa cccaggggca gaaatccgtc ggtgatactt cggcgacgct
    27841 ggcctggcgt ccgggtgaca gcggacagaa aaaactggtt cagttgttcg actccggtga
    27901 agtctgcgcg tttcgtatca aatatcccaa cggtactgtt gatgttttcc gtggctggct
    27961 gagttcactg ggtaaaacca ttgcctcaaa agacgtgatg acccgcaccg tgaaaatcag
    28021 cggtgtgggg cgtccatatc tggcagagga gggcactgaa accgtgggcg ttaccgggct
    28081 gacggtggca ccggcatccg ccagtgtcaa tgtgggagca accaccacgc tgacctttac
    28141 agtaaaacct gacggagcca gtgacaaagc gatcagtgtg cattcgacag atccacagac
    28201 tgccacggtg accctgaacg ggcttgtggc cacggtgaaa ggcgtgaagc agggcagtgt
    28261 cagcattgtg ggcatgactt ctgacggcga ttttgtggca gtggctacgg tggctgtcag
    28321 cgccgcaggt taacaggacg atactcatca tttgccccgg ttatccgggg cttttttgca
    28381 ggtggagaac atgatgtttc tgaaacaggg cacgtttaat tatgaaaagc agtccgtggt
    28441 gctcagtgag ctgtccgggc tgcagagaat tgaatatctg gcgtttgttc agcagcgaac
    28501 ggcaaagttt gatgccgaag agggagaact gccggaggct gaacgacaga ttgcttttct
    28561 gcggatgggg atggatatca atgcctggct ggtttcccgc tcactgtgga atgcggaaca
    28621 gtctcaggat gttgagacgc tttgcgcatc cgttattaca acatggtcgt atgatgccct
    28681 gggagcgggg gcggagatgg ttctgtcgct gagcggtatg ggagccattg agaatgccgg
    28741 ggatttggag catgaggtgc tgacgccgga aaagtcctga cgcgggaaat gcagtttgtc
    28801 atgcggcttg cccgggagtt ccggcgggca gactggcggc ggatgctgtc ggaaatgtcg
    28861 gccactgagc ttggtgagtg gggtgattat ttccggatgc agagtttcag tgatgtgtgg
    28921 atggatgcgc agtttgcctc gctgaaggca ttgatcgtga gagtggtgtc cggcagcagt
    28981 gatgctgcgg tggctgattt cagcctttta ccggaagaga acgggatacc ggagcgaacg
    29041 gacgaagaac tgatgcatct tggggaaggt atttccggag gtgtgcgtta tggaccagat
    29101 agccaacctg gtcattgatt tggggattga tgcggcagag tttaaaaatg aaattccccg
    29161 tatcaaaaac cttctgaatg gtgcagccag cgatgcagaa cggtcttctg cccgtatgca
    29221 gcgttttatg gagcgtcaga ctcaggctgc ccggcagaca atgcaggcgg cttcttcggc
    29281 tgcaacagcc gcatccgtcc atgcgcagac ggtggagaag agcgcacagg ctcatgaacg
    29341 catggcccgc gaggtggagc aaacccgcca gcgtatggag gcactgagcc agaaaatgcg
    29401 cgaggaacag gcgcaggcca tggctctggc ggaggctcag gataaagcgg ctgccgcgtt
    29461 ttatcgtcag attgacagtg tgaaacaggc cagtgcgggg ctgcaggaat tacagcgtat
    29521 tcagcagcag atccgacagg ccagaaacag tggcgggatt ggtcagcagg attatctggc
    29581 gctgatttct gaggttacgg cgaaaacccg tgttcttacg caggctgagg cagaggctac
    29641 ccgacagaaa gtggcgttta tccgtcagct taaagagcag gcaacccgcc agaatctttc
    29701 ttcttctgag ttgcttcgtg ctaaggctgc ccagctgggg gtaagcagtg ctgcagaagt
    29761 gtatatccgc aaaatggagc aggcaggaaa agccacgcat tcgctgggtc tgaaaagtgc
    29821 agcggcccgc caggagatag gcgttctgat aggtgaactg gctcgcggca atttaggggc
    29881 gctgagggga tccgggataa cgctggctaa ccgtgccgga tggatagaca cactgatgtc
    29941 accgaaaggc atgatgctgg gcggggttat tggcggtatt gccgcggctg tctatggtct
    30001 gggtaaagcc tggtatgacg gtcagaagga gggggaagaa tttaaccgcc agctgtcgct
    30061 gacggggcat tatgccggag ttactgccgg gcagctgtgg acgctcagtc gtgctatttc
    30121 cgggaatggt atcacgcaac atgctgcagc cggtgcgctg gctcaggtgg tggggagtgg
    30181 tgcatttcgt ggaaacgata tcggtatggt ggcgagagct gccgcacaga tggagcgatc
    30241 ggttggtcag tcggtcagcg ataccataag tcagtttaag cggctgaagg atgatcctgt
    30301 aaatgccgcg aaggctctgg acaatgagct gcattttctt actgccactc agcttgagca
    30361 gatacgcgtc cttggggaac aggggcggtc cagtgatgcg gcacggatag ccatgtctgc
    30421 actggcagag gaaaccggtc ggcgtactgc ggatattgat aataacctca atgcgctggg
    30481 tagtacgctg caaaccttgt ctgactggtg gaagcagttc tgggatgcgg ccatgaatat
    30541 tggtcgtgaa gattcgctgg atgcgcagat tgccactttg caggagaaag tgtcgcgggc
    30601 gaaaagactc ccctggacgg catcatcttc tcaggttgaa tacgatcagc agcgtcttaa
    30661 cgatcttcag gagaaaaaac gccagaagga tttgcaggat gcaaaagagc aggcagagcg
    30721 gaattatcag gagcaacaga aacgccgtaa tgctgaaaat gctgcactga atcggatgaa
    30781 tgaaacggaa gctgcacgac atcagcgtga aattgcgcgt attaatgcca tgcagtacgc
    30841 cgatcaggct gtcagggatg ctgcgataca acgtgaaaat gaacgttacg agaaagccct
    30901 ggcatccggt aagaaaaaaa cacgcgaaac ccgtaatgat gaggccaccc ggttattgct
    30961 gcagtacagc cagcaacagg cacaggtgga aggacagatt gctgctgcaa gacagtcagc
    31021 aggcattgcc actgacagga tgacagaagc gcataaacag cttctggctc tgcagcagcg
    31081 catcagcgat ctggacggga aaaaactgac ggcagatgaa aagagtgtgc tggcccgtaa
    31141 agatgaactg attcaggcac tgacgctgct ggatgtaaaa cagcaggagc ttcagaaaca
    31201 gacggcactc aacgatctga agaaaaaaac aattcagctg accagtcaac tggctgaaga
    31261 agagcgcgct cagcgtcagc aacatgacct ggatatcgcc acggtgggta tgggtgatca
    31321 gcagcggcag cgatatcagg tacaactgag tcttcgccag aaataccagc aacagctgga
    31381 gcagttgagg cgggatagtg agcagaaagg gacatataac acggatgact acagaaaggc
    31441 cgagcaggcg ctgacggaga gcctgaaccg acaactgaat gagaatcgcc gttactggca
    31501 acagcttgaa attgctcagg gtaactggaa aaacggtgct atgcgggcgt ttcagaattt
    31561 cacggcagat gcggataatg cggcaggcac tgctgagcag atgcttacag cggcatttaa
    31621 cagtgcaggt aatgcactgg caacgttctg tactaccgga aaactgaact tcaaatcttt
    31681 taccgcctcg ctcctttctg atctggcaaa aatcatggct cagatgtcca tgatgcaggc
    31741 agttaagggg attggttcgg cgtttggctg ggggagtgca gcaactgcca gtgtgacgcc
    31801 caatgctgat ggtggtgttt atcagtctgc tgatttgagt cgctacagtg gcacggtggt
    31861 taaccgtccg acgttttttg cttttgcaaa aggcgcgggt gtgatggggg aagcgggacc
    31921 tgaagccatt ctgccactgc gtcgtggtgc tgacggtaag ctgggggttg tggcggatat
    31981 tggtggttca ggtatggcga tgtttgcccc gcagtacaac atcgagatca ataacgatgg
    32041 cacgaacggg cagataggtc cggctgccct gaaggtggtt tatgaccttg ggaaaaaagc
    32101 agcagcggac tttatgcaac agcagtcccg tgatggtggt ctgttctccg gaggtggacg
    32161 atgaaaacct tccgctggaa agtgaaaccc ggtatggatg tggcttcggc cccttccgta
    32221 ataaaggtgc gctttggtga tggctattct cagcgtgcgc ctgccgggct gaacgctgac
    32281 ctgaaaacgt acagcgtgac gctttctgtt ccccgcgagg aggccacggc actggaatcg
    32341 tttctggctg agcacggggg ctggaaggcc tttctgtgga cgccgcctta tggctaccgg
    32401 cagataaagg tgacctgcgc aaaatggtcg tcgcgggtca gtatgttgcg tgttgagttc
    32461 agcgcagagt ttgaacaggt ggtgaactga tgcagaatat ccgacaggaa acactgaatg
    32521 aatgcacccg tgcggagcag tctgccagcg tggtgctctg ggaaatcgat ctgacagagg
    32581 tcggcggaga tcgttatttc ttctgtaatg agcagaacga aaaaggtgaa ccagtcacct
    32641 ggcaggggcg gcagtatcag gcctatccca ttcagggaag tggatttgag atgaacggca
    32701 aaggagccag tgcaaggcca acgctgaaag tctctaatct gcacggcatg gtcaccggga
    32761 tggcggaaga cctgcagagt ctggtcggag gaacggtggt caggcgtaag gtttacgccc
    32821 gttttctgga tgcggtgaac ttcgtcaacg gaaatagcga tgccgatccg gagcaggagg
    32881 tgatcagccg ctggcgcatc gagcagtgca gcgaactgag cgcggtgagc gcctcttttg
    32941 tactgtccac gccgacggaa acggatggcg ctgtttttcc ggggcgcatc atgctggcca
    33001 acacctgtac ctggacctat cgcggtgatg agtgcggtta tagcggtccg gctgtcgcgg
    33061 atgaatatga ccagccgacg tccgatatca cgaaagataa atgcagtaaa tgcctgagtg
    33121 gctgtaagtt tcgcaataat gtcggcaact ttggcggctt cctttccatt aacaaacttt
    33181 cgcagtaatc ccatgacaga gacagaatca gcgattctgg cgcacgcccg gcgatgtgcg
    33241 ccagcggagt cgtgcggctt cgtggtgaga acgccggagg gggaaagata ttttccctgc
    33301 gtgaatatct ccggtgagcc ggaggagtat ttccggatgt cgccggagga ctggctgcag
    33361 gcagaaatgc agggtgagat tgtggcgctg gtccacagtc atcccggtgg tttgccctgg
    33421 ctgagtgagg ccgatcggcg gctgcaggtg cagagtgatt tgccgtggtg gctggtctgc
    33481 cggggggcga tttacaagtt ccgctgtgtg ccacatctta ccgggcggtg ctttgagcac
    33541 ggggtgacgg actgttacac gctgttccgg gatgcttacc atctggcggg gattgagatg
    33601 ccggattttc atcgcgagga tgactggtgg cgtaacggtc agaatctcta tctggataat
    33661 ctggaggcca cagggctgta tcaggtgccg ttgtcagcgg cgcagccggg cgatgtgctg
    33721 ctgtgctgtt ttggttcatc ggtgccgaat catgccgcca tttactgtgg tgacggcgag
    33781 ctgctgcacc atattcctga acaactgagc aaacgagaga ggtataccga caaatggcag
    33841 cgacgcacac actccctctg gcgtcaccgg gcatggcacg catctgcctt tacggggatt
    33901 tacaacgatt tggtcgccgc atcgaccttc gtgtgaaaac gggggctgaa gccatccggg
    33961 cgctggccac gcagcttccg gcgtttcgcc agaaactgaa tgagggctgg tatcaggtgc
    34021 gcatttccgg gcgtgataca ggcgaaaacg aattatctgc ccgtcttaat gagccgctgg
    34081 caaatggtgc cgtgatccac atcgtaccgc gtctggcggg tgccaaaagt ggcggtattt
    34141 ttcaggcagt gctgggtgcg gcgctaattg ctacggcaat ctggatgccg gggcttagta
    34201 ttgttgcaag taatctcatg ttctctcttg gggcaggtat ggttttaggc ggtgttgcgc
    34261 agatgctggc accgaaagcc aaaacgccta aagtgtcagc cactgataac ggtaagcaaa
    34321 atacctattt ttcctcattg gataacatgg ttgcccaggg caatgtcctg cctgttctgt
    34381 acggtgaaat gcgcgtgggg tcacgtgtgg catctcagga gatcagcacg gcagatgaag
    34441 gggatggtgg ccaggttgtg gtaattgggc ggtaatatta tttactcatg ttctaactga
    34501 tttaatattt atatcgaaca ctgataatta ttctgttggt tagctatatg aacaaaacga
    34561 ttttattctg cacgattatt gccttaacag gatgtaaatc tttggattac gtaaaatccg
    34621 gcaagcctgt aatggaaggt aattcattaa aaaatattga tgaattgtca ggctgcatat
    34681 ccagacaatg ggctggtaat ggaacaccta taacatccat tcctattgag aatggggtaa
    34741 gccttttagt tccacaggct atgggtgggt atgatgttgt gcttgatatc aaaaaagcag
    34801 gaaatggcag tagttttact ctttatgaac gcgtaccagc attaacgcca aaaatttttg
    34861 ctgatagtgt taatgcatgt aaataatagt taatcctgcc gtaactcatg agccgccttt
    34921 tgggcggctt tgttgtttat ggagtgtgag gaatgggtaa agg
//
LOCUS       DAZPDU010000026        34131 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZPDU010000026 DAZPDU010000000
VERSION     DAZPDU010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34131
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.026"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            38..580
                     /locus_tag="ACHURS_001156"
     CDS             38..580
                     /locus_tag="ACHURS_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1236943.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAVTNFHINYY"
     gene            663..1364
                     /locus_tag="ACHURS_001157"
     CDS             663..1364
                     /locus_tag="ACHURS_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR1236944.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            1389..3988
                     /locus_tag="ACHURS_001158"
                     /pseudo
     CDS             1389..3988
                     /locus_tag="ACHURS_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            3979..5049
                     /locus_tag="ACHURS_001159"
     CDS             3979..5049
                     /locus_tag="ACHURS_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1236945.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDAFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            5061..5603
                     /locus_tag="ACHURS_001160"
     CDS             5061..5603
                     /locus_tag="ACHURS_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1236946.1"
                     /translation="MKKKTIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAASITGADVLFSGEADTLTP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            5611..6126
                     /locus_tag="ACHURS_001161"
     CDS             5611..6126
                     /locus_tag="ACHURS_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1236947.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            6092..6829
                     /locus_tag="ACHURS_001162"
     CDS             6092..6829
                     /locus_tag="ACHURS_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFR1236948.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            6940..7950
                     /gene="pyrD"
                     /locus_tag="ACHURS_001163"
     CDS             6940..7950
                     /gene="pyrD"
                     /locus_tag="ACHURS_001163"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFR1236949.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            8124..8666
                     /gene="zapC"
                     /locus_tag="ACHURS_001164"
     CDS             8124..8666
                     /gene="zapC"
                     /locus_tag="ACHURS_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFR1236950.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8663..9772)
                     /gene="ycbX"
                     /locus_tag="ACHURS_001165"
     CDS             complement(8663..9772)
                     /gene="ycbX"
                     /locus_tag="ACHURS_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFR1236951.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            10016..12124
                     /gene="rlmKL"
                     /locus_tag="ACHURS_001166"
     CDS             10016..12124
                     /gene="rlmKL"
                     /locus_tag="ACHURS_001166"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFR1236952.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNLGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRTGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWVQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHMALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            12136..14043
                     /locus_tag="ACHURS_001167"
     CDS             12136..14043
                     /locus_tag="ACHURS_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1236953.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            14173..15426
                     /gene="pqiA"
                     /locus_tag="ACHURS_001168"
     CDS             14173..15426
                     /gene="pqiA"
                     /locus_tag="ACHURS_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFR1236954.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            15431..17071
                     /gene="pqiB"
                     /locus_tag="ACHURS_001169"
     CDS             15431..17071
                     /gene="pqiB"
                     /locus_tag="ACHURS_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFR1236955.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGLE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKVRLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            17068..17631
                     /gene="pqiC"
                     /locus_tag="ACHURS_001170"
     CDS             17068..17631
                     /gene="pqiC"
                     /locus_tag="ACHURS_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFR1236956.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            17887..18054
                     /gene="rmf"
                     /locus_tag="ACHURS_001171"
     CDS             17887..18054
                     /gene="rmf"
                     /locus_tag="ACHURS_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFR1236957.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(18124..18642)
                     /gene="fabA"
                     /locus_tag="ACHURS_001172"
     CDS             complement(18124..18642)
                     /gene="fabA"
                     /locus_tag="ACHURS_001172"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFR1236958.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18711..20471)
                     /locus_tag="ACHURS_001173"
     CDS             complement(18711..20471)
                     /locus_tag="ACHURS_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR1236959.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20657..21109
                     /gene="matP"
                     /locus_tag="ACHURS_001174"
     CDS             20657..21109
                     /gene="matP"
                     /locus_tag="ACHURS_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFR1236960.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(21185..22225)
                     /gene="ompA"
                     /locus_tag="ACHURS_001175"
     CDS             complement(21185..22225)
                     /gene="ompA"
                     /locus_tag="ACHURS_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFR1236961.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(22580..23089)
                     /gene="sulA"
                     /locus_tag="ACHURS_001176"
     CDS             complement(22580..23089)
                     /gene="sulA"
                     /locus_tag="ACHURS_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFR1236962.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            23308..23937
                     /gene="sxy"
                     /locus_tag="ACHURS_001177"
     CDS             23308..23937
                     /gene="sxy"
                     /locus_tag="ACHURS_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFR1236963.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(23900..26062)
                     /gene="yccS"
                     /locus_tag="ACHURS_001178"
     CDS             complement(23900..26062)
                     /gene="yccS"
                     /locus_tag="ACHURS_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFR1236964.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(26072..26518)
                     /locus_tag="ACHURS_001179"
     CDS             complement(26072..26518)
                     /locus_tag="ACHURS_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFR1236965.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            26641..28695
                     /gene="helD"
                     /locus_tag="ACHURS_001180"
     CDS             26641..28695
                     /gene="helD"
                     /locus_tag="ACHURS_001180"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFR1236966.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GKVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(28727..29185)
                     /gene="mgsA"
                     /locus_tag="ACHURS_001181"
     CDS             complement(28727..29185)
                     /gene="mgsA"
                     /locus_tag="ACHURS_001181"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFR1236967.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(29281..29943)
                     /locus_tag="ACHURS_001182"
     CDS             complement(29281..29943)
                     /locus_tag="ACHURS_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFR1236968.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            30116..30529
                     /locus_tag="ACHURS_001183"
     CDS             30116..30529
                     /locus_tag="ACHURS_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFR1236969.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(30574..30891)
                     /gene="hspQ"
                     /locus_tag="ACHURS_001184"
     CDS             complement(30574..30891)
                     /gene="hspQ"
                     /locus_tag="ACHURS_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFR1236970.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(30949..32139)
                     /gene="rlmI"
                     /locus_tag="ACHURS_001185"
                     /pseudo
     CDS             complement(30949..32139)
                     /gene="rlmI"
                     /locus_tag="ACHURS_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
     gene            32234..32512
                     /gene="yccX"
                     /locus_tag="ACHURS_001186"
     CDS             32234..32512
                     /gene="yccX"
                     /locus_tag="ACHURS_001186"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFR1236971.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(32509..32838)
                     /gene="tusE"
                     /locus_tag="ACHURS_001187"
     CDS             complement(32509..32838)
                     /gene="tusE"
                     /locus_tag="ACHURS_001187"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFR1236972.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(32929..33588)
                     /gene="yccA"
                     /locus_tag="ACHURS_001188"
     CDS             complement(32929..33588)
                     /gene="yccA"
                     /locus_tag="ACHURS_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFR1236973.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIVHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(33795..33882)
                     /locus_tag="ACHURS_001189"
     tRNA            complement(33795..33882)
                     /locus_tag="ACHURS_001189"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33846..33848),aa:Ser,seq:tga)
BASE COUNT         8828 a         8131 c         8898 g         8274 t
ORIGIN      
        1 gcattatcat atttacgaag agatcaggat gatgacgatg aaaaaaagtg tattgacggc
       61 gtttataact gtggtatgtg caacgtccag cgttatggca gctgatgata atgctatcac
      121 ggatggcaaa gtaacattta atggtaaagt tattgctcca gcttgtaccc tggtagctgc
      181 gacgaaagat tcagtagtca cattgccaaa tgtcagcgcc accaagttgc aaactaatgg
      241 agcagtctct ggcgttaaaa ctgatgtacc aattgcctta gaaggctgtg atgttactgt
      301 aactaaaaat gcgacgttta cctttagtgg tacggctgac ggcgtgcaac ctacagcatt
      361 tgccaaccag gctaccaccg atgcagcgac gaatgttgct ctacaaatgt atctgccaga
      421 tggttctaca tcggtaactc ccggtaccga aacgtcgaat attcagttag cggattctgc
      481 tgagcaaaca gtgaccttta aagttgatta tatcgctacg gggaaagcga cttctggtaa
      541 tgtgaatgcg gtaacaaatt tccatattaa ctattattaa tagaactcat taattgtttt
      601 attaattagt acccctctag tgttctggag gggatattca tattttttaa gagtgactat
      661 ttatgaaaac ttgcataaca aagggaattg tgaccgtaag tttaacggca atattactca
      721 gctgctcatc cgcatgggcg gcaggtaaag gcgggattgg acttgcagca acacgtcttg
      781 tttattcaga aggtgaagag caaatttcac tgggtgtacg taacaccagt ccggatgttc
      841 cttatcttat tcagtcatgg gtgatgaccc cagataataa aaaatcagca gattttatta
      901 ttacaccacc gctatttgtg ctgaatccgg ctaatgagaa tctgttacgc attatgtaca
      961 ttggtgcgcc gttggcgaaa gacagagaaa cccttttctt cactagcgta cgggcagtcc
     1021 cttcaacaac gaagcgggaa gagggaaata ccctgaagat tgccacacaa agcgtcatca
     1081 aacttttctg gcgaccaaaa ggtttagcgt atcccttagg cgaggctccg gcgaaactgc
     1141 gttgcacttc gtcagctgac atggttacgg tcagtaaccc aacaccttat ttcattaccc
     1201 tgacagacct gaaaataggg ggaaaggtag ttaaaaatca aatgatttcc ccctttgata
     1261 aataccaatt ctctctgcca aagggggcca aaaatagcag cgtaacgtat cgaaccatca
     1321 atgattacgg ggcggaaacg ccacaactca actgtaaatc gtaagccgtc ttcagttaag
     1381 agagcgagat gtatagaact catcgacaac acagcctgtt aagctctggt ggagtgccat
     1441 cgtttattgg tgggctggtg gtgtttgtgt cggcagcgtt caatgcacaa gctgaaacct
     1501 ggttcgatcc tgcttttttc aaagatgatc cctcaatggt ggccgatttg tctcgtttcg
     1561 aaaaaggaca aaaaataacg ccaggggttt atcgtgtcga tattgttctg aatcagacaa
     1621 ttgtagatac gcgcaacgtc aattttgttg agataacgcc agagaagggg attgccgcct
     1681 gtttgacgac tgaaagcctg gatgcaatgg gtgttaatac tgatgcgttt ccggctttta
     1741 aacaactgga caaacaagcg tgtgtgccat tggcggagat tattccggat gccagcgtaa
     1801 cgtttaatgt gaataaactc cgtctggaaa tttcagtacc gcaaatcgcc atcaaaagta
     1861 atgcccgtgg ttatgtcccc cctgaacgtt gggatgaagg gatcaacgcg ctattactgg
     1921 gatattcgtt tagcggggct aacagtattc atagcagcgc agacagtgat tctggcgaca
     1981 gctattttct gaatttaaac agtggcgtta atttaggccc atggagattg cgcaacaatt
     2041 caacatggag tcgcagtagt ggccaaaccg cagaatggaa gaatctcagc agctatttgc
     2101 agcgggcggt tattccacta aaaggcgaac tgaccgtagg tgatgattat actgcaggcg
     2161 attttttcga tagtgtcagc tttcgtggtg tgcagctggc gtcagatgac aacatgctgc
     2221 cagacagcct gaaagggttt gcgcctgtgg tgcgtggtat cgccaaaagc aatgcccaga
     2281 taacgattaa gcaaaatggt tacaccattt accaaactta tgtatcgcct ggtgcttttg
     2341 aaattagtga tctctattcc acgtcgtcga gcggtgattt gttagttgaa atcaaagaag
     2401 cggacggcag cgtcaatagc tacagcgtac cgttttccag cgtgccatta ctccagcgtc
     2461 aggggcgaat caaatacgcg gtgacactgg cgaaatacag aaccaatagt aatgaacagc
     2521 aggagagcaa atttgcctag gccacgttgc agtggggcgg accgtgggga acgacatggt
     2581 atggtggtgg acaatatgct gaatattacc gtgccgccat gtttggtctg ggatttaacc
     2641 ttggcgattt cggagcaatt tcgttcgatg cgacccaggc gaagagtacg ctggcagacc
     2701 aaagcgaaca taaaggtcag tcatatcgtt ttctgtatgc caaaacgctc aaccaattgg
     2761 gcactaattt tcaattgatg ggctatcgct attcgacgtc gggtttctac accctttccg
     2821 acaccatgta taaacatatg gatggctacg aatttaatga cggtgatgat gaagatacgc
     2881 cgatgtggtc gcgttattac aatttgtttt acaccaaacg tggcaaactg caggtcaata
     2941 tctcccagca attaggcgag tacggttcgt tttatttaag tggtagccag caaacttact
     3001 ggcataccga tcaacaggat cggctattac agtttggcta caacacgcaa attaaagatc
     3061 tctcgctggg gggttcctgg aactacagta agtcccgtgg tcaacctgat gctgatcagg
     3121 tgtttgcact aaatttttcc ctgccgctca atctgttgct ccccagaagt aatgatagct
     3181 ataccaggaa aaaaattacg cctggatgac ctctaacacc agcatcgata acgaagggca
     3241 cattacacaa aacctgggtt taacggagac actactcgat gacggtaacc tgagctacag
     3301 cgtgcaacag ggatataaca gcgaggggaa aacggctaat ggtagcgcca gcatggacta
     3361 caaaggggcg tttgcagatg cccgagtggg ctacaactac agcgataacg gcagtcaaca
     3421 acaactgaac tacgctcttt caggcagttt agttgcccat tcacagggca ttaccctggg
     3481 gcaatcgctg ggggaaacta acgtgctgat tgcagcacca ggcgcagaga atactcgtgt
     3541 ggcgaacagc accgggctga aaactgactg gcgcggatat accgttgtcc cctatgccac
     3601 ttcttatcgg gaaaatcgaa tcgcacttga tgcggcatcg ttaaaacgta acgtggatct
     3661 tgaaaatgca gtagtcaacg tggttcccac caaaggggcg ttggttctgg cggagttcaa
     3721 tgcccatgcg ggagcaaggg tattaatgaa aacatcaaag cagggtatac cgctgcgttt
     3781 tggcgcgata gcgacgctgg acggcgtaca gactaatagc ggcattattg atgatgatgg
     3841 ctcgctctat atgtccggtt tgcccgctca gggagcgata actgtacgct ggggcgaagc
     3901 tcccgatcaa atttgtcata tcagttacca gcttaccgaa caacaaatta actctgcgat
     3961 tacgcgaatg gatgccatat gcagataatc tttggagaaa aatgcgtgtt attactacga
     4021 ctattttttg ccgccgtctt aatgctatgg tgcgctcaaa ctgctgctta tagcgggcag
     4081 tgtcatacca ctcaggggaa tccgtatatt ggcgtcaatt ttggcgttaa aaccctggag
     4141 gaagaagaaa atacggctgg agtagttaaa gacaaatttt atcagtggaa cgaatcgaat
     4201 gattattatg tttcctgtga ttgcgataaa gacaatgtca gaagtggccg atgggcattc
     4261 gccgcggatt caccgttagt ctatttaggc gacaactggt acaaaattaa tgactatctt
     4321 gccgccaaag ttttattgca ggttaaaggc agttctccta ctgcggttcc tttcgaaaac
     4381 gtgggcacag gggcagatac acgatggcat atttgcgatc ccggcggtca acgtttaggt
     4441 ggccaggggg ctagcggtaa tagcggtagc ttttccctga aaatattgca gccgttcgtt
     4501 ggttcggtcg tcattcctcc tatggcgctg gcgcgattat ttgaatgcta caacataccc
     4561 gcaggtgatt cctgcacgac tacaggtaca ccggttttag tgtattacct gtctggtacg
     4621 atcaattcac ttggctcatg ttccgtcaat gccggagaaa caatcgaggt cgatctgggc
     4681 gacgcatttg cggctaactt tcgtgttgta gggcataagc ctcttggggc cagaacggca
     4741 gaacttgcaa ttccagtcag gtgtaacacg ggaaacgcgg ggttagttaa cgtcaacctg
     4801 agtctgacgg caaccacaga ccccagctat ccccaggcga ttaagacgtc acgtcctggc
     4861 gtgggcgtgg tggtgaccga tagccagaac aacattattt cccctgctgg tggaacatta
     4921 ccgctctcta ttcctgatga tgcagacagt atcgcgcgaa tgaatgtcta tccagtcagc
     4981 acgacaggtg taccaccaga aaccgggcga tttgaagcca cggcaacggt gagaataaat
     5041 tttgattaac gcgtgaacgt atgaagaaaa aaacgatatt tcagtgcgtt atcttgttct
     5101 ttagcattct taacatccat gtcgggatgg ctgggcctga acaagttagt atgcatattt
     5161 atgggaatgt ggtcgatcag ggctgtgatg tcgccaccaa aagtgcatta caaaatattc
     5221 atattggtga ttttaatatc agtgattttc aggccgcgaa taccgtaagc actgctgctg
     5281 atttgaatat cgatatcacc ggttgtgccg ctagtattac tggcgcggac gtccttttta
     5341 gcggcgaggc tgacaccctt acgccgacac tgctcaaact aactgacaca ggcggaagcg
     5401 gtggtatggc aacggggatt gccgtgcaaa ttcttgatgc gcaaagtcag caagaaatcc
     5461 cgctcaatca ggtccagcct cttacgccct taaaagccgg ggataacaca ctcaaatatc
     5521 aacttcgtta taagtccaca aaggcgggag caacgggcgg taatgcgacg gcggttctct
     5581 attttgatct ggtttaccag tgaaggagtg atgttgaaac ggataatctg gattctgttc
     5641 ttactgggat taacgggggg ctgtgagcta tttgcccatg atggcacggt caacattagc
     5701 ggatcgtttc gccgtaatac atgcgtgctg gcacaggata gcaagcaaat caatgtgcag
     5761 ttaggcgatg tctcgctgac acgttttttt catgggaatt atggcccgga gaaatctttc
     5821 atcattaatt tgcaggattg cggaacagat gtcagcacgg ttgacgtcac cttttcagga
     5881 acccccgacg gagtgcagag cgaaatgctg tccattgaaa gtggtacgga tgcagccagc
     5941 gggctggcaa ttgcgattct ggatgatgcg aagatattga ttccgcttaa ccaggcgagt
     6001 aaagactaca gcctacatag cggtaaggtg ccattaactt tttatgcgca attgcgacct
     6061 gtcaatagcg atgtgcagtc gggtaaagtg aatgccagcg cgacatttgt acttcattat
     6121 gactaatacg tggaacagat tggcgctctt gattttcgcc gttttatcgc tgctggtggc
     6181 gggtgaattg cgggcaggag tggtggtcgg cggaacgcga tttatctttc cggcagacag
     6241 agaatcgata tctattttac tgactaatac cagtcaggaa tcctggctta ttaacagtaa
     6301 aatcaaccgc ccaacgcgtt gggccggggg ggaagcgtcg acagtgccag caccattact
     6361 ggccgctccg ccacttattc tcctgaagcc cggtactaca ggcacgttgc gcttgctgag
     6421 aacggaaagc gacatcttgc ctgtggatcg cgaaacgcta tttgagttaa gcattgccag
     6481 cgtgccatcc ggcaaagttg aaaatcagag cgtaaaagtg gcgatgcgct cggtatttaa
     6541 actgttctgg cgacccgaag gtttgccggg cgacccgctg gaagcttatc aacaattacg
     6601 ctggacacgg aattcgcagg gtgtacaact cactaaccca acgccttact acattaacct
     6661 gattcaggtg agcgtaaatg gtaaagcgtt aagtaatgcg ggagttgtgc cgcctaaaag
     6721 ccagcgtcag acaagctggt gtcaggcaat cgcaccgtgt catgtcgcct ggcgggcgat
     6781 aaatgattac ggcgggttga gtgcaaagaa ggagcaaaat ctgccctgaa acaggttcgg
     6841 aaaacgtttg cgtttttttt gccgcaggtc aattcccttt tggtccgaac tcgcacataa
     6901 tacgcccccg gtttgcacac cgggaatcca ggagagttca tgtactaccc cttcgttcgt
     6961 aaagcccttt tccagctcga tccagagcgc gctcatgagt ttacttttca gcaattacgc
     7021 cgtattacag gaacgccgtt tgaagcactg gtgcggcaga aagtgcctgc gaaacctgtt
     7081 aactgcatgg gcctgacgtt taaaaatccg cttggtctgg cagccggtct tgataaagac
     7141 ggggagtgca ttgacgcgtt aggcgcgatg ggatttggat cgatcgagat cggtaccgtc
     7201 acgccacgtc cacagccagg taatgacaag ccgcgtctct ttcgtctggt agatgccgaa
     7261 ggtttgatca accgtatggg ctttaataat cttggcgttg ataacctcgt agagaacgta
     7321 aaaaaggccc attatgacgg cgtcctgggt attaacatcg gcaaaaataa agatacgcca
     7381 gtggagcagg gcaaagatga ctatctgatt tgtatggaaa aaatctatgc ctatgcggga
     7441 tatatcgcca tcaatatttc atcgccgaat accccaggat tacgcacgct gcaatatggt
     7501 gaagcgctgg atgatctctt aaccgcgatt aaaaataagc aaaatgattt gcaagcgatg
     7561 caccataaat atgtgccgat cgcagtgaag atcgcgccgg atctttctga agaagaattg
     7621 atccaggttg ccgatagttt agttcgccat aatattgatg gcgttattgc aaccaatacc
     7681 acactcgatc gttctcttgt tcagggaatg aaaaattgcg atcaaaccgg tggcttaagt
     7741 ggtcgtccgc ttcagttaaa aagcaccgaa attattcgcc gcttgtcact ggaattaaac
     7801 ggtcgcttac cgatcatcgg tgttggcggc atcgactcgg ttatcgctgc gcgtgaaaag
     7861 attgctgcgg gtgcctcact ggtgcaaatt tattctggtt ttatttttaa aggtccgccg
     7921 ctgattaaag aaatcgttac ccatatctaa ttatttcttc gaattcctct ttataagata
     7981 atcagggctt tattttcagc cctggttgtt ttatattcat cgctgttgct tatttagaca
     8041 ttttgtactt ttattgttga ggttattaag cgaagcgaca atggattgtg ttgctgcggt
     8101 tttatagggt aggggagagg cagatgcgaa ttaaaccaga cgataactgg cgttggtatt
     8161 acgatgaaga gcacgatcga atgatgctcg atttagccaa tggtatgcta tttcgctcac
     8221 gttttgcgcg caagatgttg actccagatg ccttttctcc cgcaggcttt tgcgttgacg
     8281 acgccgcgct ctatttctct tttgaagaaa agtgccgcga ttttaattta tctaaagaac
     8341 aaaaagccga actggtgctg aatgcactgg tagcaattcg ctatctcaaa ccgcaaatgc
     8401 cgaaaagctg gcattttgtt tcccatggtg aaatgtgggt gcccatgccg ggcgatgcgg
     8461 cctgcgtttg gttgagcgat actcacgagc aggtcaattt gctggtcgtt gaatctggcg
     8521 aaaatgccgc actatgcctg ctggcacaac cctgcgttgt tattgcgggt cgcgcgatgc
     8581 agttgggcga cgccattaaa atcatgaacg acaggctgaa accgcaggtt aatgttgaca
     8641 gcttcagcct cgaacaggca gtctaacgcg ccaacttaag tgcagtcttc ggtacacagc
     8701 tacagcaaag aatggtgcca tcatcgccca ttgctgattt tttcagcggc gtgacttcgc
     8761 cttctaaaag ctgaacacgg caacttccac aaatgcccgc gcggcaagaa taagggatac
     8821 gaattccctg attttctaat tgctccagca acacctgttg gttatttcca cgaaatgcct
     8881 gtccctgcca gtcaatatct acatttgcgt ccggttgttg cgtgatgttg gcggtatcat
     8941 cagcggcagc tgcgccgtaa atttttgccg gagccgttgc cagaatttcc acctcatcgc
     9001 caacgcgaat cacgccgcta ttacgggcaa ttaaattctg accaaaatcg acatcgccgt
     9061 tatcctgggc agtgcggaaa gattgcaatg tttttaatgg ttcgcctgcc ggatgttttt
     9121 gccctttttc tgggctgacg gtggtgaaaa tacagcggct acaaggttta accacatcaa
     9181 acaccacatc accaatgcga atcactttcc agcgatcttc ttcccaggct gacgcgccgg
     9241 aaaccaccag attggggcgg aattgctcca tttttacact ggccggacaa cgttgttgga
     9301 gatcacgtaa cgaggcttcg ttagcaagaa ggtaaggata gccatcagca aatgacagag
     9361 gtacagtgtt gtggcgtttc acgcgccggg tcatttgtgg ccccacccag cgtaattgca
     9421 cttcgcggga gaaaaatcca cttagccatt tgttgatcgc gtctggcgca attcgcgcgg
     9481 taaaatgtgt gccccaaact tcggttggtg cgtcttgtgt ggcgaaatca gcaaaacgaa
     9541 catatgcgct actgccatct ggtgcggtga gatgcaagcc atcatgcaca ggtgaaggag
     9601 taaaccgtac catctgggga aactggcgag cggtaataaa cgtaccgtca ggttccgtga
     9661 tcataaagat gcgatcgaag gccagaccac tgacatcagc cagagcatgt gtaagaccaa
     9721 tgccgcgcat cgatttaaca ggatgaataa aaagccgggt taatgtcgcc acggtgcggt
     9781 cctcaaatga aaataagccc tcaactttat gacatgacgc gcttattagc tataatgcgc
     9841 aacaattttc ttagcgcctg tcccattagg ctattttatt tgtcagtttg gccctgggtt
     9901 gtgctcgaaa tcctcacgta cttgtgtacg ctccggtttc tccgcgctgt ccatgtccag
     9961 actgactgca acaattacgc ctactgcggt aggctcttag agtaaaagtg acgatatgaa
    10021 ttctctgttt gccagtacgg cccgtgggct ggaagagctg ttaaaaactg aactggaaaa
    10081 cctgggggcc gttgaatgcc aggtggttca gggtggggtc catttcaagg gcgacacacg
    10141 gcttgtttac cagagcctga tgtggagccg cctggcctcg cgtattatgt tgccgctggg
    10201 cgagtgtaag gtttacagcg atttagacct ctatctcggt gttcaggcga tcaactggac
    10261 agagatgttt aatcttggcg cgaccttcgc tgtccacttc agtggtttga atgacaccat
    10321 ccgcaacagt cagtacggcg cgatgaaagt gaaagacgcg atcgtcgatg ctttcacgcg
    10381 gaaaaatctg ccgcgtccaa atgttgatcg cgatgcgccg gatatccgcg ttaacgtctg
    10441 gctgcataaa gaaaccgcca gtatcgctct tgatctgagt ggtgatggtt tacatctgcg
    10501 tggctatcgc gatcgtgctg gtattgcgcc gatcaaagaa accctggcag ccgctattgt
    10561 gatgcgaacc ggctggcagc caggaacacc gctgctcgat ccgatgtgtg gttccggtac
    10621 gttgctgatt gaagcggcga tgctagcgac cgatcgcgca ccaggcttgc accgtgggcg
    10681 ttggggcttt agcggctggg tgcagcatga tgaagctatc tggcaggaag tgaaagcgga
    10741 agcgcaaact cgtgcccgta aaggcctggc tgagtatagc tctcattttt acggttcgga
    10801 cagcgacgca cgggtgattc aacgtgcgcg cactaacgcc cgtcttgcgg ggattggtga
    10861 actgatcacc tttgaggtga aagatgtcgc gcaactgacc aatccgctgc cgaaagggcc
    10921 gtacggcaca gtgttgagca acccgccata cggggaacgt ctggacagcg aaccggcgct
    10981 gattgcgctg catagcctgc tgggtcggat catgaaaaac cagtttggtg gctggaatct
    11041 ctctttgttt agtgcctcgc cggatctgct aagctgcttg cagctgcgtg cagacaaaca
    11101 gtacaaggcg aaaaacggcc cgctggactg cgtacagaaa aattaccacg ttgccgaaag
    11161 cactccagac agcaaaccgg cgatggtagc ggaagactac gccaaccgcc tgcgtaagaa
    11221 cctcaaaaaa ttcgagaagt gggctcgcca ggaaggaatt gaatgttacc gcctgtatga
    11281 cgccgatcta ccggaatata acgttgccgt tgaccgttat gccgactggg tggtggtgca
    11341 ggagtatgcg ccgccaaaaa ctattgatgc tcacaaagcg cgtcagcgtc tgttcgatat
    11401 tatcgctgca accatttcgg tactggggat tgcgccaaac aaactggtgc tgaaaacccg
    11461 tgaacgccag aagggcaaaa accagtacca gaaactgggc gagaagggcg agtttcttga
    11521 agttaccgaa tataacgctc acttgtgggt gaacctgacg gattatctcg atactggtct
    11581 gttcctcgat caccgtatcg cccgccgtat gctcggtcag atgagcaaag gcaaagattt
    11641 cctcaacctg ttctcttata ccggtagcgc caccgtgcat gcgggattag gcggcgcgcg
    11701 cagcaccacc accgtggata tgtcgcgtac ttatctggag tgggcagaac gcaacctgcg
    11761 tctgaatggc ctgaccgggc gtgcgcatcg cctgattcag gccgattgcc tggcgtggct
    11821 gcgtgaggca aatgaacagt ttgatctgat ctttatcgat ccgccaacct tctctaactc
    11881 aaaacgaatg gaagatgcgt ttgatgttca gcgcgatcat atggcgctga tgaaagattt
    11941 gaaacgtctg ctgcgtgcag gtgggacgat catgttctcg aacaacaaac gtggcttccg
    12001 tatggatctc gacggcctgg caaaactggg actgaaagca caagaaatta cgcaaaaaac
    12061 gctctcccag gatttcgccc gtaaccgcca gatccacaac tgctggctga ttaccgcagc
    12121 ctgaaaggaa tagtaatgtc attaatcagt atgcatggcg catggctgtc gttcagcgac
    12181 gcgccgcttc tcgataacgc agaactgcat atcgaagata acgaacgtgt ttgtctggtg
    12241 ggtcgcaacg gcgcgggcaa atcgacgtta atgaaaatcc tcaaccgtga acaagggctg
    12301 gatgacggtc gcattattta cgagcaagat ttgattgtag ctcgtctgca acaggatccg
    12361 ccgcgtaacg ttgagggtag cgtttatgat ttcgttgccg aaggcattga agaacaagcg
    12421 gaatatctga aacgctatca cgatatttcg cgcctggtga tgaacgaccc gagcgagaaa
    12481 aatctcaacg aactggcgaa ggttcaggaa cagttggatc accacaacct gtggcagctg
    12541 gaaaaccgca tcaacgaagt gctggcgcaa ctggggttag atcctaatgt tgcgctgtcg
    12601 tcgctttccg gcggctggtt gcgtaaagcg gcattaggac gcgcgctggt gagtaatccg
    12661 cgcgtgctgt tgcttgatga accgacaaac cacctggata ttgaaaccat cgactggctg
    12721 gaagggttcc ttaaaacctt caatgggacg attattttca tctcccacga ccgttcgttt
    12781 atccgcaata tggcgacgcg cattgttgat ctcgatcgcg gcaagctggt gacctatcca
    12841 gggaattacg accagtacct gcttgaaaaa gaagaagccc tgcgcgtgga agaactacaa
    12901 aatgccgagt tcgatcgcaa actggcgcag gaagaggtgt ggatccgcca ggggatcaaa
    12961 gcacgccgta cccgtaatga aggccgcgta cgcgccctga aagcaatgcg tcgcgagcgt
    13021 ggtgaacgtc gcgaagtgat gggtaccgca aagatgcagg tggaagaggc cagccgctcc
    13081 ggtaagatcg ttttcgaaat ggaagacgtt tgctaccagg ttgacggtaa gcaactggtg
    13141 aaagattttt cggcccaggt tctacgtggc gacaaaattg ccctgattgg tccgaatggg
    13201 tgcggcaaaa ccacgctgct gaaactgatg ctcggtcagc ttcaagcgga cagcgggcgt
    13261 attcacgttg gcaccaaact ggaagtggct tatttcgatc agcaccgcgc ggaactggat
    13321 cccgataaaa cggtgatgga taaccttgcc gaaggtaagc aagaggtgat ggttaacggc
    13381 aagccacgcc acgtattggg ctatttgcag gactttctgt tccatccgaa acgggcgatg
    13441 acgccggtac gtgcgctttc tggcggtgag cggaaccgct tgctgctggc gcgtttgttc
    13501 ctcaaaccaa gcaacttatt gattcttgac gaaccgacca acgatcttga tgtcgaaacg
    13561 ctggaactgc tggaagaact gatcgacagc tatcagggca cggtattgct ggttagccac
    13621 gatcgtcagt ttgtcgataa caccgttaca gaatgttgga tcttcgaagg cggcggtaaa
    13681 attggtcgtt atgtcggcgg ttatcatgat gcccgtggtc agcaagagca gtatgtggcg
    13741 ctcaaacagc ctgcggtgaa aaaaaccgaa gaagccgccg cggcaaaagc agaaactgta
    13801 aaacgcagca gtagcaaact aagctataaa ttgcagcgcg aactggagca gctaccgcaa
    13861 ttgctcgaag atctggaggc gaagctggaa gccctacaga cgcaggtggc ggatgcttcc
    13921 ttcttcagtc agccacatga gcagacacaa aaagtgctcg ccgatatggc tgctgcagag
    13981 caggagctgg agcaagcctt tgaacgctgg gagtatcttg aagcgttaaa aaatggtggc
    14041 tgatcgcaaa atagtcgatt taaacagaga cagctgacaa cggtaaaatt gcctgatgaa
    14101 ctgggtttat caggcatgaa aaccaacact ttgttagtca tctgtaaaat agcgcatcat
    14161 taaggagtac caatgtgcga acatcatcat gccgcgaagc acatcctgtg ctcgcagtgt
    14221 gacatgctgg tggcgttacc gcgccttgag catggtcaga aagcggcatg tccccggtgt
    14281 ggcacaacgt taaccgtggc gtgggatgcc cctcggcagc gtccgaccgc ctatgcgttg
    14341 gctgcactgt tcatgctgtt gctgtccaac ttgtttcctt ttgtgaatat gaacgttgca
    14401 ggagttacca gtgaaattac attactggaa attcccggcg tgcttttttc tgaggactac
    14461 gccagcctcg gcaccttttt cctgttgttt gtgcaactgg ttcccgcgtt ttgtctgata
    14521 accattctgt tactggtgaa tcgcgcggaa ttaccggtcc gtttaaaaga gcaactggca
    14581 cgggtgcttt ttcaactcaa aacctgggga atggcggaga ttttcctcgc tggtgtgctg
    14641 gtcagtttcg ttaaactgat ggcttacggc agcattgggg taggcagcag ctttctcccc
    14701 tggtgtttat tttgtgtcct gcaactgcgc gcttttcagt gcgttgatcg tcgctggtta
    14761 tgggacgaca tcgccccgat gccagaactg cgccagccgc taaaaccagg cgtcacgggg
    14821 atacgtcagg ggctgcgttc gtgctcctgt tgtacggcaa tccttcctgc tgatgaaccc
    14881 gtgtgcccgc gttgtagtac caaagggtac gttcggcgta gaaacagcct gcagtggaca
    14941 ctcgcgctgc ttgtaacgtc catcatgctg tatcttccgg ctaatatttt gcccatcatg
    15001 gtgacggatt tattaggctc gaagatgccg tcgacgattc tcgctggggt cattctgtta
    15061 tggagcgaag gctcttatcc cgtcgctgcg gtgatctttc tggccagtat tatggtgcca
    15121 acgttaaaga tgatcgccat cgcgtggctg tgttgggatg ccaaagggca tggcaagcgc
    15181 gacagtgaaa gaatgcattt gatttatgaa gttgttgagt ttgtaggccg ctggtcgatg
    15241 attgacgttt tcgttatcgc ggtgctctcg gcgctggtgc gtatgggagg tttaatgagt
    15301 atttatccgg caatgggtgc attaatgttt gctttagtcg tcataatgac aatgttttct
    15361 gctatgacgt ttgacccgcg tttgtcgtgg gatcgtcaac ctgaatcaga gcatgaggag
    15421 tcctgacagt atggaatcta ataatgggga agccaaaatc cagaaagtga agaactggtc
    15481 tcccgtgtgg atatttccta tcgtcacggc gctcattggg gcctgggttc ttttttatca
    15541 ttacagccat cagggactgg aagtgaccct gatcaccgcg aatgcggaag gaattgaagg
    15601 tggcaaaacc accattaaaa gccgtagcgt tgacgtcggc gtggttgaaa gcgccacact
    15661 ggctgatgat ttgacgcacg ttgaaatcaa agtgcggctg aattctggta tggaaaaatt
    15721 gctgcataaa gacaccgtct tttgggtggt gaaaccgcag attggtcgcg aagggattag
    15781 cggcctggga acgctgctgt ctggagttta tatcgaactg cagccaggcg cgaaaggcag
    15841 caaaatggat aaatacgatt tgctggactc gccaccgttg gccccgcctg atgcgaaagg
    15901 tatccgtgtg attctcgata gcaaaaaagc cgggcagctc tcgccaggag atccggtgct
    15961 gttccgtggc tatcgggtag gttcggttga aaccagcacc ttcgatacgc aaaaacgcaa
    16021 tatcagttat caactgttca tcaatgcacc ttatgaccga ctggtgacca gcaatgttcg
    16081 cttctggaaa gatagtggca ttgcggttga tctgacgtca gcggggatgc gtgtggagat
    16141 gggctcattg acaacgctgc ttagtggcgg tgttagcttt gatgtgccgg aaggtctgga
    16201 tttagggcag ccagtggcac cgaaaacagc tttcgttttg tatgatgatc agaagagcat
    16261 tcaggattcg ttgtacaccg atcacattga ttatctgatg ttctttaaag attcggtacg
    16321 cggtctgcaa ccgggagctc cggtagaatt ccggggtatt cgcctgggta ccgtaagcaa
    16381 agtgccattc tttgcgccga atatgcgtca gacatttaac gatgattacc gtattccggt
    16441 actgattcgt atcgagccag agcggctgaa aatgcagctt ggcgaaaatg cggatgttgt
    16501 tgagcacctt ggcgaattgt tgaaacgtgg tttacgcgga tcgctgaaaa ccggaaacct
    16561 ggtcactggt gcactgtatg ttgatcttga tttctatcca aatacgcctg caataaccgg
    16621 tattcgtgaa tttaatggtt atcagattat cccgactgtt agcggcggcc tggcgcaaat
    16681 ccagcaacga ctgatggaag cgttggataa gatcaacaaa ctgccattga atccgatgat
    16741 tgaacaggca accagtacgc tttctgaaag tcagcgcaca atgaaaaacc tgcaaacgac
    16801 gctggatagc atgaacaaga tcctcgccag ccagtcgatg cagcagttgc cgacggatat
    16861 gcagtcaacg ttgcgtgaat tgaatcgcag catgcagggc ttccagcctg gctccgcagc
    16921 ctacaacaag atggtggcgg atatgcagcg ccttgatcag gtgttgcgag aactgcaacc
    16981 ggtgctgaaa acgctcaatg agaagagtaa cgcgctggta tttgaagcga aggacaaaaa
    17041 agatccagag ccgaagaggg cgaaacaatg aaaaagtggc tagtgacgat tgcagcactg
    17101 tggctggccg gatgcagctc cggcgaaatt aataaaaact attaccagtt acctgtggtg
    17161 cagagcggta cacaaagtac cgccagccag ggcaatcgtc tgttatgggt agagcaggtc
    17221 actgttcctg actatctggc ggggaatggt gtggtttatc aaaccagtga tgtgaagtat
    17281 gtgattgcca acaacaactt gtgggccagc ccgttggatc aacaattgcg caacaccctg
    17341 gttgccaacc tgagtacgca actgcccggc tgggtggttg cctcccagcc tctgggaagc
    17401 gcccaggaca cgctcaatgt taccgtaacg gagtttaacg gtcgctatga tggcaaggtc
    17461 attgtcagtg gtgagtggct gttgaaccac cagggacaac tgatcaaacg tccgttccgt
    17521 ctggaaggag tgcaaactca ggatggttac gatgagatgg ttaaagtact ggccagtgtc
    17581 tggagtcagg aagccgcttc tattgcgcaa gagataaagc gtctacctta attataaaga
    17641 tttgtaaata taaccgtctc cggtatgttg cctgaggcgg tttttttgtt tctaacgtgc
    17701 ggaaaaagtt gttcctcttt acattttttg tacaaccgac atacccgtgt agctcacaaa
    17761 tatgacagtg gcgtgaattt tgcgcattga cggcagttat gattcgcggt attgcttaac
    17821 tgtgattgca catttagtaa tcactgtttt cttttccacc agaaaccagt atgagggaaa
    17881 cgaggcatga agagacaaaa acgagatcgc ctggaacggg cacatcaacg tggttatcag
    17941 gccggcatcg ccggacgctc aaaagaaatg tgtccctatc agacgctgaa tcaaaggtca
    18001 caatggctgg gaggctggcg agaagccatg gcggacaggg tagtaatggc ctgattctgt
    18061 ctctttaaaa agaaacctcc gcattgcgga ggtttcgcct tttgatactc tgtctgatta
    18121 taatcagaag gcagaagtat cctggaacag accgactttc aggtcgctgg cggtatagat
    18181 caaacgacca tcaaccagca cttctccatc cgccaggccc ataatcagac gacggttaac
    18241 aatgcgttta aagtgaatac ggtaggtcac ttttttcgct gtcggcagta cctgaccagt
    18301 gaatttcact tcgccaacgc ccagtgcgcg gcctttacct tcgccgccca gccagccgag
    18361 gtagaaccct accagctgcc acattgcgtc caggcccagg catcccggca taaccggatc
    18421 gccaataaag tggcatccga agaaccacag atccggattg atatccagtt ctgcttcaac
    18481 ataccctttg tcgaagttac cacccgtttc ggtcattttg accacacggt ccatcatcag
    18541 catattcggt gctggcaatt gcgggccttt agcgccaaac agttcaccgc gaccagaggc
    18601 aagaaggtct tcttttgtat aggattcgcg tttatctacc atgttctctg taagccttat
    18661 tttattgaag cacgcaggat agctaacacg tgtacgctga acaagtccga tcagttcgga
    18721 ataaaccagt tcagccaacg taatggccat ggaaaacggt gacgtccttc ctgttgcgat
    18781 gcttgcgcga tacgttcctg gatggtttgc atcagcgtcg tttggccttc gccatcccac
    18841 accagattta ataataacgg cagtgcgtca gtcacatcgt ctactgccca gatggtgaat
    18901 ttgccttctt ctaccgcttt cactagttca ctgtgaagac ttaaatggcg aacgttagct
    18961 gtggggataa tgacaccttg tttcccggtt aactcacgtt gctggcaaat agcaaagaag
    19021 ccttcgattt tctcatttaa accaccgacc ggctgggcgc gaccgaactg atcgactgaa
    19081 cctgtgatag cgatactctg attcaccggc acatcggcga gggcgcttat cagggcgcag
    19141 agttcagcca tcgaggcact atctccatca acttcactgt atgactgctc aaatgtcagc
    19201 gatgctgaga aggggatctg ttgctcaagc tgtagttccg acatcaggaa cgcttgcatg
    19261 atcatcatcc ctttcgcatg gatattgccg ccaagctccg ctttgcgttc gatgtcggtg
    19321 aattcaccat cgccaatatg cacaacgcag ctaatgcgag aaggttcgcc aaaagcgcgt
    19381 ggatgacccg gaaattcaat gaccgaaagg gcgttaattt gcccgatgcg ttcgccttcg
    19441 gtttcaatca ggatttgctc ctgaaggatc tcatcctgca tacgttcagc gaggaaacct
    19501 tcgcgccatt cacgctgctg cagcattaag tttagctgct cgccggagaa ggtgtcgcca
    19561 tcacacaggg aggcgacctc tttacactgg cggaggatcc actgcgggct aagcggaagt
    19621 gtttcttgtt caccggtgta gcgtgctgct tcgcggataa gtaccggcca ggcatccgct
    19681 cccggtgcag gtaagtgatt atgtctggcg gtaaatgtca cccagcgaca ccactgggtt
    19741 actgactccg catcgacaat ctgcagagta tcttcaaatt cgctataaat agcctgctct
    19801 gaaagctctg gctccatctc ctggaaatca gccaatgatt cgcgttcgcc taccagaatg
    19861 actttcagct tcaatggcat cgaaggcaca gagacgggga gagggcgcga ctcatcaaac
    19921 gcaacccagt caaaacgctc gcggttaacg atatttttca gccgcatcca cagcagaggt
    19981 tgcgccagca gtgtacgcaa agagatgatg agaataccgc catttgcctg atgcaccaga
    20041 ccaggctgca gggtaatgtc gccattaaac tggcgcaggc agccaaagag ttgctccgct
    20101 tctacccagt cggcagcgac aacttgcgtt aaagtcgcaa aattatcatc tgcactcact
    20161 gcgtgacgta agcggatgga gtggccggaa acctcatagt gaccgcccac cagttggcct
    20221 gcatcgcttt gtagcgtacg cgcggcattg gcaataagat tcagatactc agactcttcc
    20281 ggggccttcg ccagcataaa agaggaggat gctcgcgtat gcagcaactg ctccagcgca
    20341 aattgcagcc gcggttgagt atcactgaat aaaggatcgt tttcgtcaat caaatgtggc
    20401 tgagcaaata tttcctgata gctatcggta tcaggaacca ggtcacgcca tgcaagtttc
    20461 gtaatggtca aagttgatgt tttttagtct gttgtcaaag ccgcgattat accgtaaccg
    20521 gcactacagc acacgtagaa agcaccgaca atactccagg catgggcgtt aaagctcaca
    20581 ggatggagat tcttttcttc actggcctaa aaagctgata ttctgtaaag agttacacgg
    20641 taacattgag atcgctatga aatatcaaca acttgaaaat cttgaaagcg gttggaaatg
    20701 gaagtatctg gtgaagaagc atcgcgaagg ggagttaatc acccgttaca tagaagccag
    20761 tgccgcccag gaagccgtcg atgtgttgct ctcgctggaa aatgaacccg tgctggtaaa
    20821 tggctggatt gacaagcata tgaatccgga actggtcaat cgcatgaagc aaaccattcg
    20881 ggcaagacga aagcgccatt ttaatgcgga acaccagcat acgcgcaaaa aatcgatcga
    20941 tctggaattt attgtctggc aacgtctggc tggtcttgcg cagcgtcgcg gtaaaacgct
    21001 ttctgaaacg attgttcagc tgattgaaga tgcggaaaac aaagagaaat acgcgaataa
    21061 aatgtcttct ctgaagcagg atctgcaggc attgctgggt aaggaataac tgacgaaagt
    21121 cagttcaatt tactaaaggc aaaaaaaacc ccgcagcagc ggggtttttc taccagacga
    21181 gaacttaagc ctgcggctga gttacaacgt ctttgatacc tttaacttcg atctctacgc
    21241 gacgatccgg agccaggcag tcgatcagtg cagcacgctg tttcacgttg tcacaggtgt
    21301 tgccagtaac cgggttggat tcgcccatac cacgtgcgga gatcttgtct gccgggatac
    21361 ctttggagat caggtaatca acaacagact gagcacggcg ctcggacaga ccctggttgt
    21421 aagcgtcaga accgatgcgg tcggtgtaac ccagaacaac tacggaaccg tctttcggat
    21481 ccaggttgct cagctggctg tacagctgat ccagagcagc ctgaccttcc ggtttcaggg
    21541 ttgctttgtt gaagttgaac agaacgtcag acttcagagt gaagtgcttg gtctgtactt
    21601 ccggtgccgg agctggagcc ggagcaacta ctggagctgc ttcgccctga ccgaaacggt
    21661 aggaaacccc caggctcagc atgccgttgt ccggacgagt gccgatggtg tgtgcgtcac
    21721 cgatgttgtt ggtccactgg tattccagac gggtagcgat ttcaggagtg atcgcgtact
    21781 caacaccgcc agcgaagacc ggagaaacgc cggtgtcgtg gtttttacca taaacgttgg
    21841 atttagtgtc tgcacgccat accataccac ccagacgagt gtagatgtcc aggtcgtcag
    21901 tgattgggta acccagttta gcggtcagtt gaacgccctg agctttgtat gcaccgtttt
    21961 caacgctgcc tttgtacggc atacgaccta accagtcgta acccatttca aagccaacat
    22021 acgggttaac ctggtaacca ccaaaagcac cagcgcccag ttggttttca tgggtcgggc
    22081 cattgttgtt gatgaaacca gtgtcatggt actgggacca gcccagttta gcaccagtgt
    22141 accaggtgtt atctttcgga gcggcctgcg ctacggtagc gaaaccagcc agtgccactg
    22201 caatcgcgat agctgtcttt ttcatttttt gcgcctcgtt atcatccaaa atacgccatg
    22261 aatatctcca acgagataac acggttaaat ccttcaccgg gggatctgct caattaactc
    22321 taccgatatc ttcggcttat gccgagcacc cctggcgatg taaagtctac aacgtagttg
    22381 aaaacttaca agtgtgaact ccgtcaggca tatgaaaaaa aagtcttgta taaggtatgt
    22441 ttaatctttt ttgtcagcga caatttacag aagaaaatcg cggaaaccgc ttcagacaag
    22501 cctccgcaag gaaaattagt cacgactgaa agcattggct gggcgacaaa aaaagttcca
    22561 ggattaatcc taaatttact taatgataca aattagagtg aatttttagc ccggaaagtt
    22621 gtctcgtggc gtgagaggat gcgcttaccg gacgcataat aaaccccata gcgttacctt
    22681 catttgccgc atcaacaagt tcagcatgct cttcttcagt caaatcatct gccaaccaac
    22741 cgatcaccac actgtaattg cccgtgcgta aagcgcgaac cattgactcc acagtgtggc
    22801 aaggggagag ctggctaatc tgcattactt tcgttaaggg tagcccagat gcctgaaccc
    22861 attcccgact cagtttttgt tgcggtgtta accagagttg ccagcgcgat tgctgaccga
    22921 gttgctgtaa caatggcaac agtagaagtt gcgtcatcat gggctgatct tcgcgataga
    22981 caacttcact gataagcccg gctgtagtgt tttccgtaga gacacgcgca attttacttg
    23041 ctgcggatga gaacgacgaa gaacgatgtg catagcctga agtgtacata atcaatccag
    23101 cccctgtgag ttactgtatg gatgtacagt acatccagtg acaacaaaga tcaaccctat
    23161 tttcggaaag agcctcgcaa attttgtcgt tggtgacggg aaaacataaa ttaatcttgc
    23221 cccttaagaa taagttgcct attttcgtag ttaacggatc cgttaatgtg aatcattctt
    23281 ttatgttatg attttaaaag gaattttatg aaaagcctct cctataagcg gatctataaa
    23341 tcacaagaat acctggcaac gttgggcaca attgaatacc gatcattgtt tggcagttac
    23401 agcctgaccg ttgacgacac ggtgtttgcg atggtttctg atggtgagtt gtatcttcgg
    23461 gcttgtgagc aaagtgcaca gtactgtgta aaacatccgc ctgtctggct gacatataaa
    23521 aagtgtggcc gatccgttac cctcaattac tatcgggttg atgaaagtct atggcgaaat
    23581 caactgaagc tggtgcgtct gtcgaaatat tctctcgatg cagcgctgaa agagaaaagc
    23641 acgcgcaata cccgggaaag actgaaagat ttgcccaata tgtcttttca tctggaagcg
    23701 attctcgggg aggtggggat taaggatgta cgggcgttac gtatacttgg ggcaaaaatg
    23761 tgttggttgc gactgcggca gcaaaacagt ctggtgacag aaaagattct gtttatgctt
    23821 gaaggtgcca ttatcggcat tcatgaagct gcgctcccgg tggcacgccg ccaggagctt
    23881 gcagaatggg ctgactctct tacgccgaaa caggagtttc ctgcggaact tgagtaatct
    23941 ggcgttgcag acgaccaatc tcaggcagta atgcaatcaa taatccaact tgttgtacga
    24001 ccagaggttc tttgctgtct gcccgtggtt caagttgctg catccgctgt ttcaggctag
    24061 ctaatgcctc attgacgcgt tcttcatcag caggttgatg atgtaacgcg tcatcaacat
    24121 agcaaactgc gtcatcaaga aacgccagaa tttcaggatt agttaactgc tcccggtgag
    24181 caccgagggc tgagatatag ctggtaaacg tatggttaag gcacagcaac cgaaacgcgg
    24241 cttcgcgaat ttgcggggta acgttcggct cgctggacat atttgatacc accgacgcca
    24301 gctcagcatc acggttgtgt gcatcgcggc gggcaatacg atacgccaga cggttatcac
    24361 gcccctgatg gtattgctcc agtatggcat cgagataccg acagttggcc tctgtggcgc
    24421 gttcgagcat gcgcggcaga ttgcgaaact gccagtcagg ccagatgtag ctcactgccg
    24481 cccacgcaat ggcacaacca atcagcgtat cgattacgcg aggtaacgct acttcaaaac
    24541 cttcacccag taagttaaaa cacagtagca ccaaaagtgt gatgaacatc gttgcatgag
    24601 cgtattgcac gttacggaag gcaaaaaaga gcacgccggt aataaccagc agcaccagct
    24661 gcccttccag tgatggcaca aaccacagca caggaatgcc aatggcgata cctaccagcg
    24721 taccaataat ccttaacttc aggcggtggc gcgtggcgtt atagtttggc tggcagacaa
    24781 acaaacttgt cagcaagatc caatacccgt gatgcattcc ggttatctga atgatggcgt
    24841 agccgaagca caacaccagc gacattctta ccgcatgacg gaagagggcg gattccggcg
    24901 tgaagtgacg gctaagacgc agccagatat cactcaaccc gtgcgggctg tcatcagcga
    24961 gctcattttc gtcattatta tggggtagtg cctgggcctg ttctgattca attgttgcca
    25021 gttgggcatc aatggcgcgt aaattgttca gcaaaaatcc cagtgttttg agtaaatcgg
    25081 cgggtgcgcc gttatcgcgc atccgctcca gcgcagcatc aatatgcgta aaagcgcgct
    25141 caaaatgcgg atcatgttga taaggctgac gcaacaaaat acagcgtgac agttgctggc
    25201 acgcctggcc ctgcatcgac atcagccgct gaaaacggaa cagcacgtcg ctgtggcgaa
    25261 aatgttcacg caatgtttga tactgaatat gagaagagct ggcacgctcg tgaatatcct
    25321 gtgcgacaaa gtaataatgc agcgtgcgac gcgttccccg ttgaccacga tcgccacgta
    25381 agcgggtcag cagcgagagt ttcgtctgat tcaatgtcgc catcagcaga ccgttggcga
    25441 gagccaaatc gtacagcggt gcctggcttt catcttcaat atcaggatca aacatgcgcg
    25501 acttgagctc aagataacgc gccagttgtt catagcaacg cgccaggttg tcctgcagcg
    25561 ggcggaccgg gaacagcaga tgaccaataa gtgtcaggac gttgtaccag acggcaccgg
    25621 ccagcagata catcggctgc tgataccagt gctcatacag tgatgttccc aacatagtgt
    25681 aaatggcgat cagcaatgca ccgaaggcaa ttgttgcata gcgttgaccc agaccgccga
    25741 gcaaaatgaa gccgctggta gagagcgtta agccaatcgc aaatagccag ggccagggaa
    25801 acagcaattc tactgaggcc gaggcgataa aaaagcagaa cagcgtaatg atgaggttac
    25861 gcaaacgtcc cgccagtcgg tcatcgagat cggtcagcgc cgctgccacc atccccagcg
    25921 ttagcggaat cgtcagtttt acatcaccca gccaccacgg aaacgctgtg gttccacaaa
    25981 gcgcaataaa aatacgcgcg taatacagcc aggcgctgtt ccaggtatag cgtttgagca
    26041 aaggacttag cataaaggcc atattagtgg attattcaaa acgacgacgt gcattggctt
    26101 cacgcgcagc ttgcgctgtt tctaccgata ccacgcgacg accaaccggc catagtgcaa
    26161 tagcggcaat tttaaagttc gcaatgccga caggaatgcc aatgattgaa atacattgtg
    26221 cgatgcccgt tgcaatgtgc atcaggcata accaccagcc aaagaaaatc agccagaaaa
    26281 tattcaatac cgtaccgcca gtattcagca gcacattttt gccagccggg ttcagttcat
    26341 cgacatgaat agcttcattg ccataaggca ccagagatag tttagtgatc tcccagcagg
    26401 atcgtgtcag cggtaaggta aaaatcagca caatactgac cagagtcgcc aacagccagc
    26461 ccagagtggt ggcaaatccg ccaagcacaa agttcagaat gttcaaaacg gtacgcataa
    26521 aacctcgctt tactgtggtt ttcagtaatg ggcggcaatt gtaacgtttt tttgggctgg
    26581 agcacgtttt ctctgacggt tacactgata agaaataatt tcgtgtggat ctacagagtc
    26641 atggaactga aagcgacaac gcttggaaaa cgtctggcac agcaccctta cgatcgggcg
    26701 gtgatcctca atgccgggat taaagtctcc ggcgatcgcc acgaatacct tattcctttc
    26761 aatcaattac tggcgattca ctgtaagcgc ggtctggtat ggggcgagct ggaatttgta
    26821 ctgccggacg aaaaagtggt gcgtctgcac ggcaccgaat ggggcgagac gcagcgtttt
    26881 taccatcatc ttgatgctca ctggcggcgg tggagtggcg agatgagcga aattgcgtct
    26941 ggtgttttac gccagcaact ggatttgatt gccacgcgca ctggagaaaa taaatggctg
    27001 acgcgtgagc aaacctctgg cgttcagcaa caaatccgcc aggctttgtc ggcgttgccg
    27061 ttgccggtta accgactgga agaattcgat aactgccgtg aggcgtggcg taaatgtcag
    27121 gcctggttga aagatattga aagcgctcgg ttgcagcata accaggcgta taccgaagcc
    27181 atgcttaccg agtatgcgga ttttttccgc caggtcgagt cttcaccgct gaatccggcg
    27241 caggcccggg cagtcgttaa tggcgagcat tctctgttag tgctggcagg tgcaggaagc
    27301 ggaaaaacgt cggtgctggt ggcccgtgca ggctggttgc tggcgcgtgg tgaagcgtcc
    27361 cctgagcaaa ttttattgct ggcgtttggt cgcaaagccg ctgaagagat ggacgagcgg
    27421 attcgcgaac ggctacatac cgaagacatt accgcacgca cgtttcatgc gctggcgctg
    27481 catattattc agcagggcag caaaaaagtt ccgatagtca gcaaactgga aaatgatacc
    27541 gctgcccgtc atgaactctt tattgctgag tggcgcaagc aatgcagcga aaagaaagcg
    27601 caggcgaagg gctggcggca atggctgacg gaagaaatgc agtggtcagt gccagaaggt
    27661 aacttctggg atgatgaaaa attacagcgt cgccttgcct ctcgcctcga tcgctgggta
    27721 agtctgatgc ggatgcacgg tggtgcacag gcagaaatga ttgccagtgc acccgaagag
    27781 attcgcgatc tgttcagtaa acgtatcaag ttgatggccc cgttattaaa agcctggaaa
    27841 ggtgcgctga aggcagaaaa cgctgtcgat ttttcgggcc ttattcatca ggcgattgtg
    27901 attctggaga aaggtcgctt tatcagcccg tggaagcata ttctggttga tgaatttcag
    27961 gatatctcgc cgcagcgggc agcgttgtta gcggcattac gcaagcaaaa cagtcagacg
    28021 acgttgttcg ctgttggtga tgactggcag gcgatttacc gattcagcgg tgcgcaaatg
    28081 tcgctcacca ccgctttcca tgaaaacttt ggtgaaggcg aacgttgtga tttagacacg
    28141 acttaccgtt ttaacagtcg tatcggtaag gtggcaaacc ggtttattca gcagaaccca
    28201 ggccagctga aaaagccgct aaacagctta accaatggag acaaaaaagc cgtcacgtta
    28261 ttggatgaga gtcaacttga cgctttgctg gataagctct ctggttatgc caaaccggaa
    28321 gagcgcattc tgatcctggc gcgttaccat cacatgaggc ctgccagcct ggaaaaagcg
    28381 gcaacacgct ggccgaagtt gcaaatcgac tttatgacca ttcatgccag caaagggcaa
    28441 caggcggatt acgtcatcat cgttggcttg caggagggaa gtgatggttt tccggctgcg
    28501 gcgcgggagt cgattatgga agaggcgcta ctgccaccgg ttgaggattt cccggacgct
    28561 gaagaacggc ggttaatgta cgtggcgctg acccgggcac gccatcgggt atgggcactg
    28621 tttaacaaag agaatccctc tccctttgtg gaaatactga aaaatctgga tgtgccggtg
    28681 gcgagaaaac cgtaagaaac aggtggcgtt tgccacctgt gcaatattac ttcagacggt
    28741 ccgcgagata acgctgataa tcggggatta gaatatcgac cgcgtcgttg aaatgcggcg
    28801 actggattat gaagtctgcc gttgccacgt tggtggcgac cggaatgttc cataccgtcg
    28861 ccagacgcag caaggctttc acgtcaggat cgtgcggcac ggcatttagt ggatcccaga
    28921 agaaaatcaa tacatcaatt ttcccttctg agatcaatgc gccaacctgc tggtcacccc
    28981 ccattgggcc actcaacatc gcgttgacgt tcatgccggt cgcgcgggaa attaagttac
    29041 cggtagtgcc tgttgcatac agtacgtgtt gttccagtaa cggttgatgc cgttccaccc
    29101 agctcatcag catttgtttg cagtgatcgt gtgccaccag cgcaatatgt ttccgcgcag
    29161 gtaaagtgcg agtcgtcagt tccataatgt acatccgtag ttaactttcc tacagattac
    29221 tgtaagcact tatcgctgca agataaagac cgaaaaagcc tgcgcacagg cacaaaaatc
    29281 tcaggaagat ggttgttttt ccgcccactg caggaaagta tttcgcgttt gtgggtcagc
    29341 cagtttaaac caatacttca gccgttgttc tgtgagcacc tgagactgcg gtggaatagc
    29401 atccagctcg gaaacacccg aaagcagcgt actgtcatct gccatcatcg tggcaaattg
    29461 cggtagtgaa gcgcgtttcg cggatttgtt atagcgttca acctcgacct cataatcaat
    29521 cgtttttgcc gttgaggtaa tggcgagaat atccagcttt accggaatcg gcgtcgcatc
    29581 gccatccaac aattcaaggc gcggcgcggc atcaaaatgg ttagcttccc gctcattctc
    29641 caggcgaggc aggcgaaaat tgacctggtt gatgagctgg gtattaaaac tgaccaccag
    29701 cggtggggag atatacagcc gttcttcact attggaaaga tgaatcgtct tctcaacgcg
    29761 aaacactaac tgatgcggtc cattatccag ttcaatgctg tccgcaccgc gcagcagaga
    29821 gctggaaacc ttcttgccgt cgagtaccag aagatcgaca tcggttgaaa gccgcagcgt
    29881 ggtggcaaaa acggataccg gcaaacataa tgcaattaag gtggtcacga tgccggtttt
    29941 cataggtagc tcctgtcaaa agaccgcccg aataataatg tgtcaaaatt tttctttaat
    30001 catacttact aacatataga catatttccc gcttttgctc tcattcattc gtattagctg
    30061 catggttggc atgtcggctt tgtcgtacac tttgttaaac agtctggagg aaataatgaa
    30121 agaaaccgat attgcaggca ttttaacgtc cactcacacc attgcgctgg tgggggcaag
    30181 cgacaaaccc gatcgtccca gctatcgcgt gatgaaatat ctactcgacc aggggtatca
    30241 cgtcattccg gtttcgccaa aagttgccgg caaaacgctg ctgggccagc aggggtatgg
    30301 cacgctggca gacgttccgg aaaaggtcga tatggtcgat gttttccgta attcagaagc
    30361 ggcgtggggc gtggcacagg aagctattgc cattggcgcg aaaaccctct ggatgcaact
    30421 gggggtgatt aatgagcaag cggcagtact ggcacgggat gccgggttga atgtggtgat
    30481 ggatcgctgc ccggctattg agatccctcg cctgggcctg gccaaataaa aaatccccgg
    30541 aaggcaaaaa ccttccgggg attcgttcag ggatcagtta cgcagacgcg gggcctggag
    30601 ttgtttgcgg atggtctgcg ccagctcatc catggatggc tgttcaggat gttcatcctg
    30661 caattcgcta cttagctggg cttcagccag gtaggtatga accggtaggc cgttatcgtc
    30721 ctccatcact acgtgatacc acggagcggc gcgaagctcg tcatttaccg ccagctcatc
    30781 aggcgacggt tcagagagcg aataaaccgg gtcgatatcc acgaccactc cgagataacc
    30841 taacagggaa tggcggacct gctggccgat accgaatttg ctggcaatca tagtcacctc
    30901 ccgggaaatc tttacctttg atataagggt aaaattccac atttcaagtt acatgacgcg
    30961 acaggcaaac cctttcagat atagcccttc cggataggta gcgatcaccg gatgatcggc
    31021 tgcctgacgg aactgctcta taaattgtac atcacggccg gcatcaattg cggcatccgc
    31081 gatgattttc tgaaataaat cgctggtcat cagaccggaa caggagaaag tcaggagaat
    31141 accgccttca ttcagcaact gaatcgccag catgttgata tctttatagc cacgacacgc
    31201 gcccatcaac tggcttttat tctcaacaaa cttcggcggg tccatcacga taacgtcaaa
    31261 tttttcaccg cgatcgcgat aagtacgcag caatttaaag acatcatcac ggacaaactc
    31321 agccttgctc agatccagtt tgttcagctc aacgttctgc cgtgcaatat ccagcgcttc
    31381 ctaggaggta tcaacgctga caacctggct gcaaccgccc atcagtgccg atacggcgaa
    31441 accaccggta taggagaaac agttcagcac acgtttattt tcaacgtagc ggcgggtagc
    31501 caggcggctg tctcgctggt ccaggtagta gcccgttttg tgtccgtgct gaatatccac
    31561 cagcagcttc atgccgtgtt cttcaatcgg cagcagggca ggcggcaact cgccggtgac
    31621 gaggccctgg gtcagctcca tcccttcttt tttacgtacc gcaacatcgc tgcgatcgta
    31681 aatcgcacat tccgggtaca gcgtttgcag ggcactaatt aatgccgcgc gctgatattc
    31741 tgccccagca ctcagcagtt gcagcaccag aaaattaccg aaacgatcga tagtaatacc
    31801 cggcaggcca tcagattctc cggcgattaa acgatagctg tcgaggccat ctttttgcgc
    31861 cagtcagtca cgccattttt gtgcttgttg caaacggcgg gaaaaaaaag caatgtcgat
    31921 agactcagac gggtcaaacg tccagacgcg cgcccggatt tgcgaagctg gcgaataagc
    31981 gccgcgtgct aaccattttc cctgatgatc aacaatatcg atggtttcac cgaggctggc
    32041 tttaccttcc atgcgggcaa cggccccgga aaagacccac ggatggcgac gaagtaatga
    32101 tttttcgcgc cctttggcta acactaaacg tacactcata atttactttt ctgtcgatgc
    32161 aaagaaatgg gcgtcattgt ccggagtttt ccatggattt gcaacgcgtc cattaaggat
    32221 aaggagagca gcaatgtcga aagtctgcat aattgcctgg gtttacgggc gagttcaggg
    32281 cgtaggattt cgctacacca cacagtacga agcgaaaaga ctgggtttaa ccgggtacgc
    32341 caaaaatctt gacgatggca gcgtggaagt ggttgcctgc ggtgaagaag ggcaggtgga
    32401 aaaattaatg cagtggttaa agagtggcgg cccgcgttct gcgcgtgtag aacgggtgct
    32461 cagcgagccg catcatccct cgggggaatt aaccgatttt cgtatccgtt aaatacattt
    32521 taccggtttt ggcaggccag caattttggt ggcttgctta gccggacctt tcggaaacag
    32581 tcggtataaa tagcggctgt tacctttttc ttcgccaaat ttattcgcca tcgcttttac
    32641 cagcatacga atcgccggag aagtattgaa ttccagatag aaatcacgca caaaacgcac
    32701 cacttcccag tgttccggtg acagcgaaat cccttcgttc tctgcaatca ccactgccag
    32761 cggctcactc cactggctgc tttctttgag atagccttcg gtatccgttt ctatctcttt
    32821 accttcgaag atcagcataa ttattactac cttaatcaga ctggcggcag tgtaacaaaa
    32881 aacaaagccc cgcataaagc gaggctatga aagtgttagc gggtgagatt aatcgcggct
    32941 ggcgaagccc agaatgctca gcaggctgac gaagatgttg tacagcgaaa catacaggct
    33001 aaccgtggca cgaatatagt tcgtttcacc gccatgaacg atgttgctgg tttcaaacaa
    33061 aatagcgcca gaggagatca gaatgaagac cgcgctgatc gccagatgca gagcaggcag
    33121 ctgcaggaag atattagcaa ccataccaat cagcaccacc acaatacccg ccatcagcat
    33181 accgccgagg aacgacatat ctttgcgggt ggtcagcaca tatgcagagc agcagaagaa
    33241 cactaacgcc gttccgccca gtgccatagc gattacgtca cccattccgg cagacagata
    33301 ggtgttcaga ataggtccga ggatataacc cagaaaaccg gtaaaggcga atgcggagat
    33361 aatcccggtc ggcttattcg ccgttttata ggtcaggaac atcaaaccat acatacccac
    33421 cagcgtcaga atcagacccg gagatggcag catcagcacc gtactggcag ttgcggtaat
    33481 cgccgaaaag gccagcgtca ggctcagcag aaaataggta ttacgcagca ccttatgggt
    33541 gctaagcagt gatgtacggt catgtgaaga actaacaata cgatccattg agtcactctc
    33601 tatgacagat gtaattaatt aagcagcata atgataatgc gtaagggcac ccagaagttt
    33661 tacccatctt tacgcatttg atctggaaca ggtttaacag cggattatca ggtcattaag
    33721 caaatataac gccctgagaa tttcgacagg caaaagaaaa aggggttagc atttagctaa
    33781 ccccttatct tatttggcgg aagcgcagag attcgaactc tggaaccctt tcgggtcgcc
    33841 ggttttcaag accggtgcct tcaaccgctc ggccacactt ccggaatgac gcgcactata
    33901 aacatcccga tgcggcgtgt aaacccctaa tttgtttgtt tgcctgaaaa acagccaaaa
    33961 gtgcattgat agcgtgaaat aacagcagat tgatcatttc atcaccatga attccttctc
    34021 ttttactcgt ttagcaaccg gctaaacatc cccaccgccc ggcaaaaaga aaaataggtc
    34081 catttttatc gctaaaagat aaatccacac agtttgtatt gttttgtgca a
//
LOCUS       DAZPDU010000027        33607 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZPDU010000027 DAZPDU010000000
VERSION     DAZPDU010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33607
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.027"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            19..255
                     /locus_tag="ACHURS_001190"
     CDS             19..255
                     /locus_tag="ACHURS_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFR1236974.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            258..1115
                     /locus_tag="ACHURS_001191"
     CDS             258..1115
                     /locus_tag="ACHURS_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFR1236975.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     RVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            1136..3127
                     /locus_tag="ACHURS_001192"
     CDS             1136..3127
                     /locus_tag="ACHURS_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFR1236976.1"
                     /translation="MRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLDEPYWAMT
                     SAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSAWLGFCTW
                     ACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGILCGGMMM
                     MILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQILTMNLLRI
                     QAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREILEQLLTAL
                     ASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRLQSGVDDH
                     ARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLAAISCVLY
                     SAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLATMQLLKLQ
                     MPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNENLAKIVGVALAWLAFAILRPGSD
                     ARKSRRHIRALRRDFVDQLSRHPTLSGSEFESLTYHHVSQLSNSQDALARRWLLRWGV
                     VLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATLEELQRIC
                     DSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(3128..3649)
                     /gene="sodC"
                     /locus_tag="ACHURS_001193"
     CDS             complement(3128..3649)
                     /gene="sodC"
                     /locus_tag="ACHURS_001193"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFR1236977.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(3730..4626)
                     /locus_tag="ACHURS_001194"
     CDS             complement(3730..4626)
                     /locus_tag="ACHURS_001194"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFR1236978.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLKSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(4675..4914)
                     /locus_tag="ACHURS_001195"
     CDS             complement(4675..4914)
                     /locus_tag="ACHURS_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFR1236979.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            5017..5616
                     /gene="nemR"
                     /locus_tag="ACHURS_001196"
     CDS             5017..5616
                     /gene="nemR"
                     /locus_tag="ACHURS_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFR1236980.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKIAEVPKGS
                     FYHYFRSKEAFGVAMLERHYATYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGHENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRNSEPLENALAHVKTIIATPAV"
     gene            5653..6750
                     /gene="nemA"
                     /locus_tag="ACHURS_001197"
     CDS             5653..6750
                     /gene="nemA"
                     /locus_tag="ACHURS_001197"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFR1236981.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMEE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRRAELNPQRAESFYGGGAEGYTDYPTL"
     gene            6831..7238
                     /gene="gloA"
                     /locus_tag="ACHURS_001198"
     CDS             6831..7238
                     /gene="gloA"
                     /locus_tag="ACHURS_001198"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFR1236982.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            7341..7988
                     /gene="rnt"
                     /locus_tag="ACHURS_001199"
     CDS             7341..7988
                     /gene="rnt"
                     /locus_tag="ACHURS_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFR1236983.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            8081..12650
                     /locus_tag="ACHURS_001200"
                     /pseudo
     CDS             8081..12650
                     /locus_tag="ACHURS_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(12701..13048)
                     /gene="grxD"
                     /locus_tag="ACHURS_001201"
     CDS             complement(12701..13048)
                     /gene="grxD"
                     /locus_tag="ACHURS_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFR1236984.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGKLVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            13382..14197
                     /gene="mepH"
                     /locus_tag="ACHURS_001202"
     CDS             13382..14197
                     /gene="mepH"
                     /locus_tag="ACHURS_001202"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFR1236985.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            14325..14906
                     /gene="sodB"
                     /locus_tag="ACHURS_001203"
     CDS             14325..14906
                     /gene="sodB"
                     /locus_tag="ACHURS_001203"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFR1236986.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(15052..16227)
                     /locus_tag="ACHURS_001204"
     CDS             complement(15052..16227)
                     /locus_tag="ACHURS_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1236987.1"
                     /translation="MKINYPLLALALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAA
                     GMLISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSR
                     ILTSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGW
                     RMSFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGA
                     MFTLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLL
                     LLMVIMLATPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIG
                     AFNLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(16393..16482)
                     /gene="cydH"
                     /locus_tag="ACHURS_001205"
                     /gene_synonym="ynhF"
     CDS             complement(16393..16482)
                     /gene="cydH"
                     /locus_tag="ACHURS_001205"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFR1236988.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            16781..17806
                     /gene="purR"
                     /locus_tag="ACHURS_001206"
     CDS             16781..17806
                     /gene="purR"
                     /locus_tag="ACHURS_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFR1236989.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLVGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(17803..18735)
                     /gene="punR"
                     /locus_tag="ACHURS_001207"
     CDS             complement(17803..18735)
                     /gene="punR"
                     /locus_tag="ACHURS_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFR1236990.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            18848..20059
                     /gene="punC"
                     /locus_tag="ACHURS_001208"
     CDS             18848..20059
                     /gene="punC"
                     /locus_tag="ACHURS_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFR1236991.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGMTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIIPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYCGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEAGCQNH
                     GNAEVAHSESH"
     gene            20350..21498
                     /gene="cfa"
                     /locus_tag="ACHURS_001209"
     CDS             20350..21498
                     /gene="cfa"
                     /locus_tag="ACHURS_001209"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFR1236992.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(21538..22179)
                     /gene="ribE"
                     /locus_tag="ACHURS_001210"
     CDS             complement(21538..22179)
                     /gene="ribE"
                     /locus_tag="ACHURS_001210"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFR1236993.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            22394..23767
                     /gene="mdtK"
                     /locus_tag="ACHURS_001211"
     CDS             22394..23767
                     /gene="mdtK"
                     /locus_tag="ACHURS_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFR1236994.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNKKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(23808..25064)
                     /locus_tag="ACHURS_001212"
     CDS             complement(23808..25064)
                     /locus_tag="ACHURS_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFR1236995.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            25372..25448
                     /locus_tag="ACHURS_001213"
     tRNA            25372..25448
                     /locus_tag="ACHURS_001213"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25406..25408,aa:Val,seq:gac)
     gene            25453..25529
                     /locus_tag="ACHURS_001214"
     tRNA            25453..25529
                     /locus_tag="ACHURS_001214"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25487..25489,aa:Val,seq:gac)
     gene            25637..25942
                     /locus_tag="ACHURS_001215"
     CDS             25637..25942
                     /locus_tag="ACHURS_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFR1236996.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            26068..27672
                     /locus_tag="ACHURS_001216"
     CDS             26068..27672
                     /locus_tag="ACHURS_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFR1236997.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EYGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(27684..28496)
                     /gene="ydhT"
                     /locus_tag="ACHURS_001217"
     CDS             complement(27684..28496)
                     /gene="ydhT"
                     /locus_tag="ACHURS_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFR1236998.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIVTAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(28500..29285)
                     /gene="phsC"
                     /locus_tag="ACHURS_001218"
     CDS             complement(28500..29285)
                     /gene="phsC"
                     /locus_tag="ACHURS_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFR1236999.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMCGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(29282..29950)
                     /locus_tag="ACHURS_001219"
     CDS             complement(29282..29950)
                     /locus_tag="ACHURS_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR1237000.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQENKYYQYQLLGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(30014..30652)
                     /locus_tag="ACHURS_001220"
     CDS             complement(30014..30652)
                     /locus_tag="ACHURS_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFR1237001.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(30665..32767)
                     /locus_tag="ACHURS_001221"
     CDS             complement(30665..32767)
                     /locus_tag="ACHURS_001221"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFR1237002.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            complement(32788..33414)
                     /locus_tag="ACHURS_001222"
     CDS             complement(32788..33414)
                     /locus_tag="ACHURS_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFR1237003.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
BASE COUNT         8048 a         8869 c         8285 g         8405 t
ORIGIN      
        1 cgtaaaggaa tctactttgt gaagtttatg ctcaatgcaa caggattgcc cttacaagat
       61 ctggtgttcg gtgcgtccgt ctattttcct ccgtttttca aagcattcgc gtttggattc
      121 gtcatctggc ttgtcgtaca ccgcctgctt cgtggctgga tctacgccgg tgacatctgg
      181 catcccttgt taatggattt atcgctgttt gcgatttgcg tttgccttgc tctggcaata
      241 ctgattgcgt ggtaactatg tcaattaaaa caattaagta tttctcaaca atcattgtag
      301 cggtagttgc ggttcttgcc ggatggtggc tgtggaatta ttacatgcaa tctccgtgga
      361 cacgcgacgg gaaaatacgc gcagaacagg tttctatcac cccacaggtg tcgggacgta
      421 ttgttgagct gaatataaaa gacaaccagc tggttaacgc aggggatctt ctgctcacga
      481 tcgataaaac gccctttcag atcgccgagc tgaacgcaca ggctcagctg gcgaaagcgc
      541 aatctgacct cgcaaaagcc aataacgaag cgaatcgtcg tcgtcatctc tcacaaaatt
      601 ttatttctgc cgaagaactc gataccgcta acctcaatgt taaagcgatg caggccagtg
      661 ttaatgccgc acaggccacg ctgaaacaga cacaatggca actggcgcaa acggaaattc
      721 gcgccccggt gagtggacgg gtgactaacc tcactacccg catcggcgat tacgctgaca
      781 ccgggaaacc gctttttgcc cttgtcgaca gccactcgtt ttacgtcatt ggttattttg
      841 aagaaaccaa gttgcgccat atccgcgaag gtgcaccagc acaaattacc ctctatagtg
      901 ataacaaaac gttacagggt cacgtttcca gtatcggtcg ggcgatttat gatcagagcg
      961 ttgaaagtga ctcaagcctt attccggatg tgaaacctaa cgtcccctgg gttcgtctcg
     1021 cccaacgcgt tcccgtccgc tttgcgctgg ataaagttcc aggcgatgtc acgctggtgt
     1081 cgggcaccac ctgtagcatt gccgtaggtc aataatgaac gcatcgtaat ggtccttgcg
     1141 caatttgccc tggttcaggg ccacgctggc gcaatggcgt tatgcgttac gcaataccat
     1201 tgccatgtgt ctggcgctga cggttgccta ttatttgaat ctggatgaac cctattgggc
     1261 gatgacctcg gctgcagtgg ttagctttcc caccgttggc ggtgtcatca gcaaaagcct
     1321 cggacgcatc gctggcagtt tactgggagc cattgcggca ctgcttcttg ccggacatac
     1381 gctcaatgag ccgtggtttt ttctattgag catgtcggcg tggcttggct tttgtacctg
     1441 ggcctgtgcg cacttcacga ataacgtcgc gtatgcattt caactggcgg gctacacggc
     1501 tgccatcatc gcctttccga tggttaatat tactgaggcc agccagctgt gggatatcgc
     1561 tcaggcgcgc gtttgcgagg tgatagtcgg tattttgtgc ggcggcatga tgatgatgat
     1621 cctgcctagc agttccgatg ctactgccct tttaactgca ttgaaaaaca tgcacgcccg
     1681 attactggaa catgccagtt tactctggca gcctgaaaca aacgatgcca ttcgtgcagc
     1741 acatgagggg gtgattgggc agatactgac catgaatttg ctgcgtatcc aggctttctg
     1801 gagccactat cgttttcgcc agcaaaacgc gcgccttaat gcgctactcc accagcaatt
     1861 acgtatgacc agtgtcatct ccagcctgcg acgtatgttg ctcaactggc cctcaccgcc
     1921 aggtgccacg cgagaaattc tcgaacaatt gctgactgcg ctcgccagtt cgcaaacaga
     1981 tgcttacacc gtcgcacgta ttatcgcccc gctacgcccg accaacgtcg ccgactaccg
     2041 gcacgtcgcc ttctggcagc gactacgtta tttttgccgc ctttatctgc aaagtagtca
     2101 ggaattacat cgtctgcaaa gcggtgtaga tgatcatgcc agactcgcac ggacatccgc
     2161 tctggctcgt cataccgaca acgccgaagc tatgtggagc gggctgcgta cattttgtac
     2221 gttgatgatg attggcgcat ggagtattgc atcgcaatgg gatgccggtg ccaatgcatt
     2281 aacgctggca gcaattagct gcgtactcta ctccgccgtc gcagcaccgt ttaagtcgtt
     2341 gtcactgctg atgcgcacgc tggtgttact ttcgctattc agctttgtgg tcaaatttgg
     2401 cctgatggtc cagattagcg atctgtggca atttttactg tttctctttc cactgctggc
     2461 gacaatgcag cttcttaaat tgcagatgcc caaatttgcc gcactgtggg ggcaactgat
     2521 tgtttttatg ggttcgttta tcgctgtcac taatcccccg gtgtatgatt ttgctgattt
     2581 tcttaacgag aatctggcaa aaatcgttgg cgttgcgctg gcttggctag cgttcgccat
     2641 tctgcgtcca ggatcggatg ctcgtaaaag ccgccgccat attcgcgcgc tgcgccggga
     2701 ttttgtcgat cagctaagcc gccatccaac actgagtgga agcgaatttg aatcgctcac
     2761 ttatcatcac gtcagtcagt tgagtaacag ccaggatgcg ctggctcgcc gttggttatt
     2821 acgctggggt gtagtgctgc tgaactgttc tcatgttgtc tggcaattgc gcgactggga
     2881 atcgcgttcc gatccgttat cgcgagtacg ggataactgt atttcactgt tgcggggagt
     2941 gatgagtgag cgtggcgttc agcaaaaatc actggcggcc acacttgaag aattacagcg
     3001 gatttgcgac agccttgccc gtcatcatca acctgccgcc cgtgagctgg cggcaattgt
     3061 ctggcggctg tactgctcgc tttcgcaact tgagcaagca ccaccgcaag gtacgctggc
     3121 ctcgtaatta cttaattaca ccacaggcat agcgttcacc gccaccgccc agcggtttag
     3181 gttgatcgga catattatcg ccgccaacgt ggaccatcag cgccttgtct ttgatttcat
     3241 ccagtgattt cagacgaggc gcgatgacgg catcggtagc tttgccgtca ttattgacga
     3301 ccagtgcagg cagatcgcct aaatgcccgg taccttctgg cccttcatgt ttaccggtat
     3361 tttgtggatc aagatgcccg cctgcggatt ccgcggcgct ggctttgcca tctttggtgg
     3421 ctggctggca gcttcctttg gcatgaatat ggaagccatg ttcaccgggg ggtaatgctt
     3481 tcagatcggg tgaaaactcc agacctttat cggtttcagt aatggtgacg ctaccaattg
     3541 actgcccaac cccttgcgac gtgacgaggt tcatctcgac tttttcactg gcagcttgtg
     3601 cgccggtcgc aacaaccagc gccagaatgg ccagactaaa acgtttcata ggacctccgt
     3661 tcattgtgca ttcctgaata agtgtaaacc agtgacacat ttttgaacgg cggctattcc
     3721 taaaagtgct tacggtacgt cgtaccccag tgccgcttta cggatacgaa accattgttg
     3781 acgggtcatt ttcagtgttt ctgcttcgac agctgcccgt acgcgctcaa ttttacctga
     3841 gccgataatt ggcagcggct gcgacggtaa acgtaatacc caggcgtaaa ccacctgttc
     3901 aatcgagccc gcgtttaact cctccgccac cacagccagt tcatcacgca gcggctggaa
     3961 ataatcatca ttaaacagac gaccaccacc aaggcaggac cacgccatcg gacgaacacg
     4021 cagttgttgt agttggtcga gcgtgccatc cagcagtaac ggctgatgca ccggggatat
     4081 ttccacctga ttagtggcaa gggtaaacgg cagacgtgat ttcaacaggg caaattgcgc
     4141 aggcgtaaag ttcgatacgc caaaatgacg cactttgccg ctctgatgca gatgtttgaa
     4201 cgcgtccgcc acttcatcgg catccattaa cgggtctggt cggtggatta acagcagatc
     4261 cagatgatcg gtcgcgagat taattagcga ctgttcggcg ctcttaatga tgtgatcgcg
     4321 gtcagtgatg taatgaccaa tgacgttttc ttcacgcgcg gtcgtcgcga taccgcattt
     4381 actgacgatt tccatccgtt cacgcaggtg aggtgccagt ttcagtgcct cgccaaacgc
     4441 cgcttcgcac tgatagccac cataaatatc agcatggtcc acggtggtca cgccgagatc
     4501 cagatgctct tcaataaaac tgaccagctg gcgggcggac atattccagt ccatcaatcg
     4561 ccagtagccc atcacaaaac gggaaaactc cgggccttgc ggcgcaatag taatacgctg
     4621 aaccataatc gcttcctctt atcagatatg agaggagtat acgcaagatt aggttcaaaa
     4681 gagtgatggt tgctccggtt cgtctgatga cgctggctta tttgcgcgta atttgcgcat
     4741 taatcgctgc cgacaaaggc gcagcacctc ttgtttttcg ccatcgctca ttttattcca
     4801 gttaaaacgc tcatcccgac tacgaaaaca gccgcgacaa aacccgcgtt cgtcagactg
     4861 gcaaattccc cggcacgggc tctggacggg aaagaactct aattgctccg ccacttcgcc
     4921 ctcctcaaat aagattatta ccattattga agctgttaat gtccaaagta gcaactttgc
     4981 ttgcactaga ccgactggtc tactacactc caacgcatga acaaacacac cgaacatgat
     5041 actcgcgaac atctcctggc gacgggcgag caactttgcc tgcaacgtgg gttcaccggg
     5101 atggggctaa gcgaattact aaaaatcgct gaagtgccga aagggtcctt ctatcactac
     5161 tttcgctcta aagaagcgtt tggcgttgct atgcttgagc gccattacgc cacatatcac
     5221 cagcgactga ctgagttgct gcaatccggc gaaggtaact accgcgaccg catactggct
     5281 tattaccagc aaacactgaa ccagttttgc caacatggaa ccatcagtgg ttgcctgaca
     5341 gtaaaactct ctgccgaagt gtgcgatctg tcagaagata tgcgtagcgc gatggataaa
     5401 ggcgctcgcg gcgtgattgc cctgctctct caggcgctgg aaaatggcca tgagaaccac
     5461 tgtttaacct tttgtggcga accgctgcaa caggcacaag tgctttacgc actatggctg
     5521 ggtgcgaatc tgcaggccaa aatttcgcgc aattccgagc cactggaaaa cgcgctggca
     5581 catgtaaaaa ccattattgc gacgcctgcc gtttagcagg cattttttat caccagacga
     5641 ccgggagcct ttatgtcatc tgaaaaactg tattccccac tgaaagtggg cgcaataact
     5701 gcggcaaacc gtatttttat ggcaccgctg acgcgtctgc gcagtattga accgggtgac
     5761 atccctaccc cgttgatgga ggaatactat cgccaacgtg ccagtgccgg tttgattatt
     5821 agcgaagcca cgcaaatttc tgcccaggca aaagggtatg ctggtgcacc tggcatccat
     5881 agtccggaac aaattgccgc atggaaaaaa attaccgctg gcgttcatgc tgaaaatggt
     5941 catatggccg tacagctgtg gcacactgga cgcatttctc acgccagcct gcaacctggc
     6001 ggtcaggcac cggtagcgcc ttcagcactt agcgcgggaa cacgtacttc tctgcgcgat
     6061 gaaaatggtc aggcgatccg tgttgaaaca tccatgccgc gtgcgcttga actggaagag
     6121 attccaggta tcgtcaatga tttccgtcag gccattgcta acgcgcgtga agccggtttt
     6181 gatctggtag agctccactc tgctcacggt tatttgctgc atcagttcct ttccccttct
     6241 tcaaaccatc gtaccgatca gtacggcggc agcgtggaaa atcgcgcacg tttggtactg
     6301 gaagtggtcg atgccgggat tgaagaatgg ggtgccgatc gcattggcat tcgcgtttcg
     6361 ccaatcggta ctttccagaa cacggataac ggcccgaatg aagaagccga tgcactgtat
     6421 ctgattgaac aactgggtaa acgcggcatt gcttatctgc atatgtcaga accagattgg
     6481 gcgggaggtg aaccgtatac tgatgcgttc cgcgaaaaag tacgcgcccg tttccacggt
     6541 ccgattatcg gcgcaggtgc atacacggta gaaaaagccg aaacgctgat tggcaaaggg
     6601 ttaattgatg cggtggcatt tggtcgtgac tggattgcga acccggatct ggtcgcccgc
     6661 ttgcagcgca gagctgagct taacccacag cgtgccgaaa gtttctacgg tggcggcgcg
     6721 gaaggctata ccgattaccc gacgttgtaa tccaacattg cgagcggcgt aaagccgccg
     6781 ctatactaaa acaacatttt gaatctgtta gccattttta ggataaaaag atgcgtcttc
     6841 ttcataccat gctgcgcgtt ggcgatctgc aacgctccat cgatttttat accaaagtgc
     6901 tgggcatgaa actgctgcgt accagcgaaa acccggaata caaatactca ctggcgtttg
     6961 ttggctacgg cccggaaacc gaagaagcgg tgattgaact gacctacaac tggggcgtgg
     7021 ataaatacga actcggcact gcttatggtc acatcgcgct tagcgtagat aacgccgctg
     7081 aagcgtgcga aaaaatccgt caaaacgggg gtaacgtgac ccgtgaagcg ggtccggtaa
     7141 aaggcggtac tacggttatc gcgtttgtgg aagatccgga cggttacaaa attgagttaa
     7201 tcgaagagaa agacgccggt cgcggtctgg gcaactaatc tcctgccggg cgtgaactca
     7261 tcgcgcccgc atctttactg catcgacaag taatatttgt cataatgcgc gctgcaattt
     7321 atccgtatta agagaatcag atgtccgata acgctcaact taccggtctg tgcgaccgtt
     7381 ttcgtggttt ttatcctgtt gtgatcgatg ttgaaacagc cggatttaac gccaaaaccg
     7441 atgcgctgct tgagattgcc gccatcaccc tgaaaatgga tgaacaaggc tggctgatgc
     7501 cggacaccac attacatttc cacgtcgaac catttgtcgg cgcaaatttg cagccagagg
     7561 ccctcgcctt caacggcatt gacccgaacg atcccgatcg cggcgcggtc agcgaatacg
     7621 aggcgctgca cgaaattttt aaagttgtac gtaaaggtat taaagcgagc ggctgtaacc
     7681 gcgccattat ggtggcgcac aatgccaatt ttgatcacag ctttatgatg gccgccgcag
     7741 aacgcgcctc actgaaacgt aacccgttcc accctttcgc cacttttgac actgctgcac
     7801 tggccgggct ggcactcgga caaaccgtat tgtcaaaggc ttgccagacc gctggcatgg
     7861 acttcgacag cacccaggcg cactccgcgc tgtacgacac cgaacgcact gctgtgctgt
     7921 tttgtgaaat cgtcaaccgc tggaaacgtc tgggaggctg gccgctacct gccgccgaag
     7981 aggtgtaatc gagtcgatgc ctgatgacat gcaatgattc aggcatctat agtgaggcta
     8041 ttccacgcat caagcatgat attcacgggg aatagcgtta atggcagata atccagaccc
     8101 ttcatcgctc ctgccggacg tgttttcact ggcgacccgc gactggtttc ttcgcgcctt
     8161 taaacagccg accgctgtcc agtcgcaaac ctggcatgtg gcggcgcgag gcgaacatgc
     8221 gctggtgatt gcaccgactg gctccgggaa aacgctggca gcatttctct acgccctcga
     8281 tcggctcttc cgcgaaggcg gcgaagatac ccgcgaggcg cataagcgta aaacctcacg
     8341 catcctctat atttcaccga taaaagccct gggcaccgac gttcagcgca acttgcagat
     8401 cccgttgaag ggtattgccg atgaacggcg gcggcgcggc gaaacggaag tcaatcttcg
     8461 cgtagggatc cgtactggcg atacgcctgc acaggaacgc agcaaactca cccgtaatcc
     8521 gccggatatt ctgatcacca cacccgaatc actctatctg atgctgacct cccgcgcgcg
     8581 cgaaacgcta cgcggcgtcg aaacggtaat tattgatgaa gtccacgcgg tagcgggcag
     8641 taaacgtggt gcgcatctgg cgttaagtct ggagcggctc gatgcgctgc tccacacctc
     8701 agcacagcga attggccttt ctgccactgt gcgctcagcc agcgatgtgg cagcatttct
     8761 tggtggcaat cgcccggtta cggtagtcaa cccgcccgca atgcgccatc cgcagatacg
     8821 aattgtcgta ccggtcgcca atatggatga tgtctcatcg gtcgccagca gcaccggcga
     8881 agacagccat gccggccggg aaggctccat ctggccatat attgaaacgg gtatccttga
     8941 tgaagtgttg cgccatcgct cgaccattgt ctttactaat tcacgtgggc tggcggaaaa
     9001 actgacggca cgattaaatg agctttacgc cgcacgctta cagcgttccc cgtctatcgc
     9061 cgttgatgcg gcccatttcg agtcgacctc cggcgcaacc tctaaccgtg tacaaagtag
     9121 cgacgttttt attgcccgct cacaccacgg ctccgtctct aaagaacaac gagcaatcac
     9181 cgagcaggcg ctgaaatcgg gtgagttacg ctgcgtggtc gcaacctcca gtctcgaact
     9241 ggggattgat atgggcgcag tggatctgat gattcaggtg gcaacgccgc tttctgttgc
     9301 cagtgggtta caacgcattg gtcgcgccgg acatcaggtt ggcggtgtat ctaaagggct
     9361 gtttttccct cgtacccggc gtgacttagt cgattccgca gtcattgtag agtgtatgtt
     9421 cgcaggcagg ctggaaaacc tgacaccgcc gcataatcca ctcgacgtcc ttgcgcagca
     9481 aaccgttgcc gccgcggcga tggatgcatt acaggtagac gaatggtact cccgcgtacg
     9541 ccgtgccgca ccgtggaaag atctgccaag acgtgttttt gacgctacgc tggatatgct
     9601 ttccgggcgc tatccctctg gcgatttttc tgcttttcgc cccaaactgg tctggaaccg
     9661 ggagaccggg atattgaccg cccgacctgg cgctcaattg ttggcggtta ccagcggtgg
     9721 caccattccg gatcgtggca tgtatagcgt gttattaccc gaaggtgaag aacaggccgg
     9781 ttcgcggcgg gtgggtgaac tggatgagga gatggtatat gagtcgcggg tgaacgacat
     9841 tatcactctc ggcgctacct catggcggat ccagcaaatc acccgcgatc aggtgattgt
     9901 gactcctgct ccgggtcgtt ctgcccggct ccccttctgg cgtggtgaag gtaacggacg
     9961 tccggctgaa ttaggcgaga tgatcggcga ttttcttcat ttgctggcgg atggcgcgtt
    10021 cttttccggg actattcccc cgtggctggc agaagaaaat acgatcgcca atattcaggg
    10081 gttgattgac gagcaacgca acgcgacggg catcgttccg gggagtcgcc atctggtcct
    10141 cgaacggtgc cgtgatgaaa ttggcgactg gcgtattatt ttgcactctc cctatggaag
    10201 acgggtgcat gaaccctggg cgctggcgat tgccgggcga atacatgccc tatggggcgc
    10261 tgacgcgtct gtggtcgcca gtgatgacgg cattgttgcg cgcattcctg acactgatgg
    10321 taaattgccc gacgccgcga tttttttgtt tgaaccagaa aagttgctgc aaattgtccg
    10381 cgaggcggta ggcagctcgg cacttttcgc cgcccgtttt cgcgaatgcg ccgcgcgggc
    10441 attattaatg ccggggcgca ctccgggcca tcgcaccccg ctttggcaac aacggctgcg
    10501 cgccagtcag ttgctggaaa tcgctcaggg atatccggat tttccggtca ttctcgaaac
    10561 cctacgcgaa tgtctgcaag atgtttatga tcttcccgca ctggaacgtt tgatgcgtcg
    10621 cctgaacggt ggcgaaattc aaatatccga tgtaacgacc actacgccct cgcctttcgc
    10681 cacaagttta ttgttcggct atgtcgcgga atttatgtac cagagcgacg ccccgctggc
    10741 agagcgccgg gcatccgtac tgtcgctgga cagcgagtta ctgcgcaatc tactcggaca
    10801 ggtcgatccg ggggaattac tcgacccgca ggtcattcgc caggtggaag aagagttgca
    10861 acgactggct cctggcagaa gagcgaaagg tgaagaagga ttgttcgacc tgctgcgcga
    10921 actggggcca atgaccgttg aagacctggc gcaacggcat acaggcagca gtgaagaggt
    10981 tgcgtcgtat ctggaaaatc ttcttgcagt aaaacgaatc ttcccagcga tgattagcgg
    11041 acaggagcgt cttgcctgta tggatgatgc cgccaggctg cgtgatgccc tcggcgtacg
    11101 actaccagag tcattgccag agatttattt acatagagtc agttacccgc ttcgcgacct
    11161 ctttctgcgc tatctccggg ctcatgctct ggtcacggct gaacaactgg ctcatgagtt
    11221 tagtctcggt attgccattg tcgaagagca gcttcagcaa ctgcgtgaac agggtctggt
    11281 gatgaatctg caacaagaca tctgggtgag cgatgaagta tttcgtcgtc tgcgtttgcg
    11341 ctcgctgcaa gccgccagag aagcgacgcg tcccgttgca gccacgacct atgcgcgatt
    11401 gctgctggaa cgtcagggcg tattacccgc caccgatggt agcccggcgc tctttgcctc
    11461 aacatcgcca ggcgtttatg agggcgtaga tggcgtgatg cgggtgatcg aacagcttgc
    11521 cggagtcggt ttacccgcct cactctggga aagccagatc ctgcctgccc gcgtacgcga
    11581 ctattcgtca gaaatgctcg atgaattact ggcaaccggt gcggttatct ggtcggggca
    11641 aaaaaactgg gtgaagatga cggcctggtg gcactgcatc tacaggaata tgctgcagaa
    11701 tcgttcactc ccgacgaagc ggatcaggcg aatcgttcgg cgctgcaaca agcgatagtc
    11761 gctgttctgg ctgacggagg agcctggttt gcacaacaaa tcagccaacg gatacgcgac
    11821 aaaatcggcg aatcggttga tctctctgcc ctgcaagagg cgttatgggc gctggtctgg
    11881 caaggcgtca tcaccagcga catttgggca ccgttacgcg ccctcacccg cagcagttcc
    11941 aacgcacgca cctcaactcg ccgcagtcac cgggctcgtc gtggacgtcc tgtctatgcg
    12001 caacccgtct cgccgctggt atcttacaac acaccaaatc tggctggatg ctggcgctgg
    12061 cggaaaatat gctcgaccgc tacggcatca tcagtcgtca ggcggtgata gccgaaaata
    12121 tccctggcgg gtttccatcg atgcaaacgc tttgtcgaag tatggaagac tccgggcgaa
    12181 ttatgcgagg tcgttttgta gaaggtctgg gtggcgcgca attcgctgaa cgtctgacta
    12241 ttgaccgatt gcgcgatctg gcgacacaag ccacgcaaac gcgccactat acaccagtgg
    12301 cgctctctgc caacgatccg gctaatgtgt ggggaaatct tctgccctgg cctgcacatc
    12361 cggcaacgct ggttccaacg cgtcgggcgg gtgcgctggt ggtcgtttct ggcggcaaat
    12421 tgttactcta tctggcgcaa ggtggcaaaa aaatgctggt ctggcaggaa aaagaggaat
    12481 tactcgcccc agaggttttc cacgcgctga ctaccgcact gcgtcgcgaa ccacggctgc
    12541 gctttacgct aacagaagtg aatgatctac cggtccggca aacgccgatg tttacgctgc
    12601 tgcgtgaggc gggattttca agttcgccac aagggctgga ttggggatag agaaaggact
    12661 gacggatgcc cgttcgcatc cgtcagtatt gcaggacgga ttattccgcg tccggctctt
    12721 cagacttgta tttagcggca gtttctttga tcagctgctg cagttcgcca cgctgataca
    12781 tttcgatcac gatatcacaa ccgccgacca gcttgccgtc aacccacagt tgcgggaagg
    12841 tcggccagtt agcatatttc ggcagttccg cacgaatgtc cggattctgc agaatatcaa
    12901 cataggcaaa acgttcgcca catgcggcaa gcgcctggac tgcctgggca gagaaaccgc
    12961 agctcggcag tttcggtgaa cctttcatgt acagcaggat cgggttttca gcaatctggc
    13021 gttggatttt ttcgatagtg gtgctcattg tcttgcttcc tcaaacttct ttacggcagt
    13081 aatctgacat tgtagcgggt cagtgcggca tcggaaaata acattttcat cacgcttttg
    13141 ctattttatc cctttgctct atttgttgca tttcaaatat tcgtttcttc tctgatgccg
    13201 gcaaactggc gtatttataa ccatttttag ctttcactgc tgcatttttt tgcgctcgcc
    13261 aacgaaacgt attttttaac aataaaagct attaactttc tcttcttcta tgcattagaa
    13321 tcatcaagtt ttgtaaatca gacgcaggca tgatagacct gccttaacag agggacgctc
    13381 agtggcgcgg ataaaccgta tttcgatcac gctctgtgct ttacttttta ccaccctgcc
    13441 tttaacgcct atggcccatg cttcaaagca agccagggag agttctgcta ccactcatat
    13501 caccaaaaaa gcagataaaa agaaaagcac ggcaaccacc aaaaagaccc agaaaacagc
    13561 gaaaaaagcc gccagtaaaa gtacgaccaa aagcaaaacc gcttcttccg ttaaaaaatc
    13621 ttccattacc gcttctaaaa acgccaaaac tcgcagcaaa cacgccgtca ataaaacggc
    13681 ctcagccagc tttaccgaaa agtgtaccaa acgtaagggc tataaatcgc attgtgtgaa
    13741 agtcaaaaat gccgcgtcag gaactcttgc cgacgcgcac aaagcgaagg tgcaaaaagc
    13801 tacgaaagtg gcaatgaata aactgatgca gcaaattggt aagccatatc gttggggtgg
    13861 cagctcaccg cgtaccggtt ttgattgcag cggcctggtt tattacgctt ataaagattt
    13921 ggtgaaaatt cgtattccgc gtacggcgaa tgaaatgtat cacctgcgtg atgcagcgcc
    13981 aatcgaacgt agtgaactga aaaacggcga cctggtcttt ttccgtactc agggacgcgg
    14041 cacagccgat catgtcggcg tgtatgtcgg caacggcaaa tttattcagt caccgcgcac
    14101 aggtcaggaa attcaaatca cttctctcag tgaagactac tggcagcgcc actatgttgg
    14161 cgctcgtcgg gtaatgaccc caaaaacact tcgctaaaac tttaccctgt tgttacggca
    14221 acagggtaag ttcatctttt gtctcacctt ttaatttgct accctatcca tacgcacaat
    14281 aaggctattg tacgtatgca aattaataat aaaggagagt agcaatgtca ttcgaattac
    14341 ctgcactacc atatgctaaa gatgctctgg caccgcacat ttctgcggaa actatcgagt
    14401 atcactacgg caagcatcat cagacttatg tcactaacct gaacaacctg attaaaggta
    14461 ccgcgtttga aggtaaatca ctggaagaga ttattcgcag ctctgaaggt ggcgtattca
    14521 acaacgcagc tcaggtctgg aaccatactt tctactggaa ctgcctggca ccgaacgccg
    14581 gtggcgaacc gactggaaaa gtcgctgaag ctatcgccgc atcttttggc agctttgccg
    14641 atttcaaagc gcagtttact gatgcagcga tcaaaaactt tggttctggc tggacctggc
    14701 tggtgaaaaa cagcgatggc aaactggcta tcgtttcaac ctctaacgcg ggtactccgc
    14761 tgaccaccga tgcgactccg ctgctgaccg ttgatgtctg ggaacacgct tattacatcg
    14821 actatcgcaa tgcacgtcct ggctatctgg agcacttctg ggcgctggtg aactgggaat
    14881 tcgtagcgaa aaatctcgct gcataataac tgatggcaaa tgcagcattg cctgatgcgc
    14941 ttcgcttacc atgccaacgc ggatgaggcg ttaacagcgc attcggcaag ataaatcgca
    15001 cgttgtcagc aactgtaacg cagaaggtta tccttctgcg tttttgttta attagctgtt
    15061 ggcaacgcaa actgtttcag gttgttttct ggctgacata aacaccagca ataatgccag
    15121 tcccgcgaca atcgccccca tcaccggcac aaagctgtat cccagcccag cggaaattac
    15181 cgcaccacca gccgctgctc ccagcgcatt tccaagatta aaggcaccaa tattgactga
    15241 tgaagacaga cctggcgctt cactggcgac acgcatcacg cgcatctgta acggcggtac
    15301 gaccgcaaag gttgctgcgc cccacaccac catgctaata gctgcgccga actcattgcg
    15361 ggccaggaac ggggttgcca gcataatcac catcagcagc aacaaaaagc ctttcaacgt
    15421 gccgttaact gaacgatctg ccagtttgcc gccgagatag ttaccgatag agaatccgac
    15481 accaatcagc accagcattg ccgtgacgaa caccggtgtt gcgtgggtaa tactttgcag
    15541 taccggagag atataggtgt agagagtaaa cattgcgcca gctcccagta ccgtcgtcag
    15601 caatgcagac agcacctgcg gacgcattaa taccgccagc tcttttttca cttcaggtcg
    15661 tgcccctgta ccacctttag gtaatgagaa gaacagactt accattgaaa tcactcccag
    15721 ccccgccgtt gccagaaatg acatccgcca gccgatggtt tcacccaacc aggtcgccgc
    15781 cggcacgcca ccgatatttg ccagggttaa ccccataaac atagtggcaa ctgcgctggc
    15841 ctgtttatgt tttggcacca cgcttgcggc cacgactgaa cccaaaccaa aaaatgctcc
    15901 gtgattcagg ctggtcaaaa tgcgtgaaag catcagggtc atataatccg gcgcgatggc
    15961 ggaaagtacg ttgccgagcg tgaaaattgc catcaggaaa atcaacgcac tgcggcgggc
    16021 acgatgagaa agtagaagcg tcatcagcgg cgcgcccacc attacgccaa ctgcataggc
    16081 actgattaac attccggcag cgggaatcga gacatccaca ccgcgcgcaa tgacgggcaa
    16141 caagcccatt ggcgagaact ccgttgtccc gataccaaac gcgccaatcg ccagcgccag
    16201 cgccagcaac ggatagttaa ttttcatgcc ttatctccac ctcttcgcgt cattacgcga
    16261 tattcattaa agtggcgaaa gcatgacagc aatcacaaaa aaatgaaaat aacaaaaaga
    16321 gaaagcactt ttgccatttt gctaacaaac aggaaggaga tgcgagggag aacgcgctcc
    16381 ctcgagagga aatcagtgca gcgcggcagt caaacccacg gctacgatca aaccgaggac
    16441 gataatcgtt gttaccagtg aaaatttaag gtcggtgctc atcaagtttt ctcctttttt
    16501 attaccacac aaaaagtgat attacgcatt tttacacact gtgatgaaaa aatctcccgt
    16561 catttataat gataagtgtt tttaccactt ccccttttcg tcaagatcgg ccaaaattcc
    16621 acgcttacac tatttgcgta ctggccattg accccttcct gacgctccgt gtcgtttttc
    16681 cggcgtaccg caacactttt gttgtgcgta aggtgtgtaa aggcaaacgt ttaccttgcg
    16741 attttgcagg agctgaagtt agggtctgga gtgaaatgga atggcaacaa taaaagatgt
    16801 agcgaaacga gcaaacgttt ccactacaac tgtgtcacac gtgatcaaca aaacacgttt
    16861 cgtcgctgaa gaaacgcgca acgccgtgtg ggcagcgatt aaagaattac actactcccc
    16921 tagcgcggtg gcgcgtagcc tgaaggttaa ccacaccaag tctatcggtt tgctggcgac
    16981 cagcagcgaa gcggcctatt ttgccgagat cattgaagca gttgaaaaaa attgcttcca
    17041 gaaaggttat accctgattc tgggcaatgc gtggaacaat cttgagaaac agcgggctta
    17101 tctgtcgatg atggcgcaaa aacgcgtcga tggtctgctg gtgatgtgtt ctgagtaccc
    17161 agagccgttg ctggcgatgc tggaagagta tcgccatatc ccaatggtgg tgatggactg
    17221 gggtgaagca aaagctgact tcaccgatgc ggtcattgat aacgcgttcg aaggcggcta
    17281 catggccggg cgttatctga ttgaacgcgg tcaccgcgaa atcggcgtta tccccggccc
    17341 gctggaacgt aacactggcg caggccgcct tgtcggtttt atgaaggcga tggaagaagc
    17401 aatgatcaag gtgccggaaa gctggattgt gcagggtgac tttgaacctg aatccggtta
    17461 tcgcgccatg cagcaaatcc tgtcgcagcc gcatcgccct actgccgtct tctgtggtgg
    17521 cgatatcatg gcaatgggcg cactttgtgc tgctgatgaa atggggctgc gcgtcccgca
    17581 ggatgtttcg ctaattggtt atgataacgt gcgcaacgcc cgctatttta cgccggcgct
    17641 gaccacaatc catcagccaa aagattcgct gggtgaaaca gcgttcaaca tgctgttgga
    17701 tcgtatcgtc aacaaacgtg aagaaccgca gtccattgaa gtgcatccgc gcttgattga
    17761 acgccgctcc gtggctgacg gcccgttccg cgactatcgt cgttaatcac ccgttgcggg
    17821 agtctcttcc ggctcccgca gccactcctt attcagcgtc tcactatcgc cgagatactc
    17881 aagcaaccag gttaacgcag gcgacatatc attttgctgc catgtcagac aacatgccga
    17941 atccggaaag gggttttcca gttcaagtgc tacccacttc ccctcattaa gccacggttt
    18001 ggcgaaatgt gttggcacca tccctatgca taatcctgcc gagatacagg ttgccgatga
    18061 ttcccagtca ggcacgacaa ctcttttttg gttatccagc aaccaggtaa tacgtttagg
    18121 tagcgttcgc gaggtgtctt cgcgcaccaa cgacggccag ttgcgcaacg tatcatcgct
    18181 gaacgggcca tccatcaacg ccagcgggtg atggctggca acaacgcaac tccagcttag
    18241 catccccata tcccggaagg cataacgacc gcctaccgga atcgcgcgtg ttgcgccaat
    18301 cgccagttcc acgcgcccgt cggaaagcgc atcccagaca ccgttgaaca cttcctgaaa
    18361 gacaagaagt tcgacatcat caaaatggcg ataaaaatca acgatcatct gccgtgtacg
    18421 ttctggcctg acaatattat ccactgcgat agctaactga ccgcgccagc cgttcgctat
    18481 ctgctgacat tgctggcggg tgatctgcat ttttttgaca acagagcgcc cttctttgag
    18541 aaaccacgct ccagcagcgg tcagctccac atcacggtgc cgtcgttcaa agagcggcac
    18601 cgccagccac tcttccagct gacgcacggt atagctgacc gcagaaggaa cgcgatgcag
    18661 ctcctgtgcc gcagcgctaa aactaccatt acgcgctacc gcatcaacaa cttcgagtga
    18721 atattctgac cacatagtct gcctgcaaaa tttttgaaac caatcatcaa atattaccgt
    18781 ttcacaacac taatttcact ccctacactt tgcggcggtg tttaattgag agatttagag
    18841 aatatacatg caacctggga aaagattttt agtctggctg gcgggtttga gcgtactcgg
    18901 ttttctggca accgatatgt atctgcctgc tttcgccgcc atacaggccg acctgcaaac
    18961 gcctgcgtct gctgtcagtg ccagccttag tctgttcctt gccggttttg ccgcagccca
    19021 gcttctgtgg gggccgctct ccgaccgtta tggtcgtaaa ccggtattat taatcggtat
    19081 gacaattttt gcgttaggta gtctggggat gctgtgggta gaaaacgccg ctacgctgct
    19141 ggtattgcgt tttgtacagg ctgtgggtgt ctgcgccgcg gcggttatct ggcaagcgtt
    19201 agtgacggat tattatcctt cacagaaagt taaccgaatt tttgcgacca tcataccgct
    19261 ggtgggtcta tctccggccc tggctcctct gttaggaagc tggctgctgg tccatttttc
    19321 ctggcaggcg attttcgcca ccctgtttgc cattaccgtg gtgctgattc tgcctatttt
    19381 ctggctcaaa cccacgacga aggcccgtaa caatagtcag gatggtctga cctttaccga
    19441 cctgctacgt tctaaaacct attgcggcaa cgtgctgata tacgcggcct gttcagccag
    19501 tttttttgca tggctgaccg gttcaccgtt catccttagt gaaatgggct acagcccggc
    19561 agttattggt ttaagttatg tcccgcaaac tatcgcgttt ctgattggtg gttatggctg
    19621 tcgcgccgcg ctgcagaaat ggcaaggcaa gcagttatta ccgtggttgc tggtgctgtt
    19681 tgctgtcagc gtcattgcga cctgggctgc gggcttcatt agccatgtgt cgctggtcga
    19741 aatcctgatc ccattctgtg tgatggcgat tgccaatggc gcgatctacc ctattgttgt
    19801 cgcccaggcg ctgcgtccct tcccacacgc aactggtcgc gccgcagcgt tgcagaacac
    19861 tctacaactg ggtctgtgct tcctcgcaag tctggtagtt tcctggctta ttagtatcag
    19921 cacgccattg ctcaccacca ccagcgtgat gttatcaaca gtagtgctgg tcgcgctggg
    19981 ttacatgatg caacgttgtg aagaagctgg ctgccagaat catggcaatg ccgaagtcgc
    20041 tcatagcgaa tcacactgat ctatatcgat atacttatac ttaggctgct aacaaaattt
    20101 tgttgtatga ttgaaattag cggcctatac taatttcgag ttgttaaagc tacgataaat
    20161 attatgtttt tacggggaca ggatcgttcc cgactcacta tggatggtca tttcggcaag
    20221 ggttcctcct ttccctctgt tctacgtcgg attatagact cgcggttttt tctgcgagat
    20281 ttctcacaaa gcccaaaaag cgtctacgct gttttaaggt tctgatcacc gaccagtgat
    20341 ggagaaacta tgagttcatc gtgtatagaa gaagtcagtg taccggatga caactggtac
    20401 cgtatcgcca acgaattact tagccgtgcc ggtatagcca ttaacggttc tgccccggcg
    20461 gatattcgtg tgaaaaaccc cgattttttt aaacgcgttc tgcaagaagg ctctttgggg
    20521 ttaggcgaaa gttatatgga tggctggtgg gaatgtgacc gactggatat gttttttagc
    20581 aaagtcttac gcgcaggtct cgagaaccaa ctcccccatc atttcaaaga cacgctgcgt
    20641 attgccggcg ctcgtctctt caatctgcag agtaaaaaac gtgcctggat agtcggcaag
    20701 gagcattacg atttgggtaa tgacttgttc agccgcatgc ttgatccctt catgcaatat
    20761 tcctgcgctt actggaaaga tgccgataat ctggaatctg cccagcaggc gaagctcaaa
    20821 atgatttgtg aaaaattgca gttaaaacct gggatgcgcg tactggatat tggctgcggc
    20881 tggggcggac tggcacacta catggcatct aattatgacg taagcgtggt gggcgtcacc
    20941 atttctgccg aacagcaaaa aatggctcag gaacgctgtg aaggcctgga tgtcaccatt
    21001 ttgctgcaag attatcgtga cctgaacgac cagtttgatc gtattgtttc tgtggggatg
    21061 ttcgagcacg tcggaccgaa aaattacgat acctattttg cggtggtgga tcgtaatttg
    21121 aaaccggaag gcatattcct gctccatact atcggttcga aaaaaaccga tctgaatgtt
    21181 gatccctgga ttaataaata tatttttccg aacggttgcc tgccctctgt acgccagatt
    21241 gctcagtcca gcgaacccca ctttgtgatg gaagactggc ataacttcgg tgctgattac
    21301 gatactacgt tgatggcgtg gtatgaacga ttcctcgccg catggccaga aattgcggat
    21361 aactacagtg aacgctttaa acggatgttt acctattatc tgaatgcctg cgcaggtgct
    21421 ttccgcgccc gtgatattca gctctggcag gtcgtgttct cacgcggtgt tgaaaacggc
    21481 cttcgagtcg ctcgctaaag gctattctat cgccccctct ccgggggcga tttcagatca
    21541 ggcttctgta cctggttgat tcatggcatt ttctcgtgcc gccagcacac gttctaccgt
    21601 atctaccact gcctgagttt gtggatcaat ttcaatgttg acgcgtgcgc cgagtttttt
    21661 cttcccaaga gtcgtgcgtt ccagagtttc cggaattaaa tggacgcaaa aacgcgttgg
    21721 cgtgacttcg ccgacggtca ggctaatacc gtcgatgcca ataaatcctt tgtacaggat
    21781 atatttcatc aactgactat cctggacttt aaaccagatc tggcgattat tttccgaggt
    21841 taatattttc gccacttcag cggtggtcat aatatgacct gacattaagt gtccgccaat
    21901 ttcatcgctg aattttgccg cacgctcaac gtttacccaa tcccccactt ttaaatcgcc
    21961 aagattggta atgcgtaacg tttctttcat caggtcaaaa ctgacatggt tgccgttaat
    22021 ttccgtcacg gtcaggcagc aaccgttatg cgccacggaa gcacctgttt ccaggccgtc
    22081 cagcatgtgg tcgggtaact ccaccacatg cgtacgaaaa tttggtttct cgtcaatcga
    22141 caccagtttt gcggtgccct gtacaatccc cgtaaacata cttacaactc ctgaaatcag
    22201 ttaagacatt ctgttcagca caatagcagg tggaaaacgc ccttaccagt gaaggggtaa
    22261 gaatgactat tttttcactg gagaattaat aaatcctcgc tacaatagac tgaatttccc
    22321 ctgcttcttc tttttgctgc ccattcaggc ggctttttag tctctcatat aactacaaat
    22381 aaaaggtgtt cacgtgcaga agtatatcag tgaagcgcgt ctgttattag cattagcaat
    22441 cccggtgatt ctcgcgcaaa tcgcccaaac tgcgatgggt tttgtcgata ccgtgatggc
    22501 gggcggctat agtgccaccg acatggcggc ggtcgctatc ggtacttcta tctggcttcc
    22561 ggcgatcctc tttggtcacg gactgctgct ggcattaacg ccggttatcg cgcaattaaa
    22621 tggttccggt cgacgtgagc gcattgcgca tcaggtgcga caaggtttct ggctggcagg
    22681 ttttgtttcc gttctcatta tgctggtgct gtggaatgca ggttacatta tccgctccat
    22741 ggaaaacatc gatccggctc tggcggacaa agccgtgggt tatctgcgtg cgttgttgtg
    22801 gggcgcgcca ggctatcttt tcttccaggt tgcccgtaac cagtgtgaag gtctggcaaa
    22861 aaccaagccg ggtatggtaa tgggctttat cggcctgctg gtgaacattc cggtgaacta
    22921 tatctttatt tatggtcatt tcggtatgcc tgagctcggt ggcgttggtt gtggcgtggc
    22981 tactgcggcg gtgtattggg ttatgttcct tgccatggtt tcttacatta aacgcgcccg
    23041 ctccatgcgc gatatccgta acaaaaaagg caccgcaaaa cccgatcctg cggttatgaa
    23101 acgactgatt caactcggtt tgccgattgc gctggcacta ttctttgaag tgacactgtt
    23161 tgccgtcgtg gctctgttag tgtctccgct cggtattgtt gatgtcgcag gacaccagat
    23221 tgccctgaac tttagttcac taatgttcgt gcttccaatg tcgctggcgg cagcggtaac
    23281 tatccgcgta ggttatcgtc tgggtcaggg ctcaacgctg gatgcgcaaa ccgctgctcg
    23341 gaccgggctt atggtgggtg tctgtatggc aaccctgacg gccattttca cggtttcact
    23401 gcgggagcaa atcgccctgt tgtacaacga caatcccgag gttgtaacgc tggctgcgca
    23461 tttgatgttg ctggcggcgg tatatcagat ttctgactca atccaggtga ttggcagtgg
    23521 gattttgcgt ggttataaag atacgcgttc cattttctat atcaccttta cagcttactg
    23581 ggtgctgggc ttgccaagcg gctatattct ggcactgacc gatctggtcg ttgaacctat
    23641 ggggccagca ggcttctgga taggctttat tatcggcctg acatcggcag ccattatgat
    23701 gatgctgcgt atgcggttcc tgcaacgtat gccgtcagcc atcattctgc aacgagcgtc
    23761 ccgctaataa agacaaggcg caaccttcac gggttgcgcc tgtgttttta cgcaggctgg
    23821 agcgttgcgc caatcccgtc ttcgtctggc tgtaatttca gagcgttaca cagagtttgc
    23881 cgactggcat ctgttatctc aacgcgtata tccccgttaa cttccccttt cacattgatg
    23941 aagtcaggtg aaatttcttt cgcactggtt tgcaggcaaa tcgctccctc tccgcgcagg
    24001 cgtttcactt tcagttgcac atgcgttgcc gcaccagtga tcagccacag ttttccgcca
    24061 ttcagattga tgttaccaac aatgaaagtg acgctgtcac cttttaccgt actgccgtcg
    24121 agaatcaaca caccgccctg ctgtacagta atcgttgatt gttgcagctc tgcgccataa
    24181 ccaataatca ttgaagcgcc gtccagtaat gcaacactcc cggtcagttc aaccggtgcg
    24241 cgatcttcct ctacgacaaa attttcgtcc gcgctggtgg gtgacaacag gatcactgtt
    24301 ccaccttgta ttaccgcatt ttccagcgta ccggcatgca cttcagcccg cccccccaca
    24361 ttcaccgaca ggttttgcgt cttaccggaa ctgtagagct gaaggccatc cgtccccaga
    24421 cgataaatgg atccattctc aacaatagtg tcagagtctt caccaccgag attattcatg
    24481 gtgccaccag caagcaacgt atcactggct ttcccggcca gcataaaaac gccgccctga
    24541 ttaatctcac aattgctggc ttctccactt ggatacacca acaattcacc accttcatct
    24601 accacgaccg ctgaggttgt cccattaacc aacaggccgc cctcttgatc gagaatgatt
    24661 ttttccgcgc gatgtccttc cagtacacgc aggttaccgc cattttccag caacaaaccg
    24721 caggcataac cctgatcaac gctgaattca ccttcgggat ggcggccatt aaccgtagcg
    24781 agcgtagaga gactaattgc gcctccctcg gactgaacaa tatctgtggc aatggcaccg
    24841 tcgctgaccg atagcgtgcc attgttaccg tactgtgtgc atttggcgat cccaccggtt
    24901 gccactttca accagccagc ccccgtgacc acggtatttt cggctcttcc gccagcttca
    24961 acaatcaact cgccttcagt aagttgcgtc gcgccaatga cctcgccggt cttaacaatt
    25021 tgcacattcc cgtcgctcat caagtttttc gcatcagatc ccatgattta ttcctttgct
    25081 gcatctgtgt gcctttattg ctacctaagt gtaaaggcta cggaggattt atccacgaca
    25141 gatttgagat ggtggcaaac aattctgttt aaactctgat acacgaatta ttgggttgta
    25201 tcagatgtaa atgcgatcct gaataaaaat cgcccttgca aatcaacaaa atatagccaa
    25261 tttgcttaaa agctctgcaa acggtcgaaa tagcgcagaa aattacgttt tgcctcttgc
    25321 cacctttcca ctctgccgct aatatttgtc cccgttgtca cctacaacgt tgcgttcata
    25381 gctcagttgg ttagagcacc accttgacat ggtgggggtc gttggttcga gtccaattga
    25441 acgcaccatc ctgcgtccgt agctcagttg gttagagcac caccttgaca tggtgggggt
    25501 cggtggttcg agtccactcg gacgcaccag attttcttaa tctggtcttt tcctttttcc
    25561 ctctgtttat tctctgtatc caatacgtta aaagatttac actgtcttcg tatgcgttat
    25621 cagaaggaga atcgctatgg caactttgtt acaacttcat tttgctttta atggcccatt
    25681 tggcgacgca atggctgagc agcttaaacc acttgctgag tcgattaatc aggaacctgg
    25741 ttttctgtgg aaggtatgga cagaaagtga aaagaaccac gaagccggtg ggatctacct
    25801 tttcactgat gaaaaaagcg cccttgccta tctggaaaaa catactgccc gactgaaaaa
    25861 tctcggcgtt gaggaagttg tcgccaaagt tttcgatgtc aatgagccac ttagtcaaat
    25921 caatcaggca aaactcgcct gacagaattt aatcaagggc ggttagcgcc cttttcatcc
    25981 ctgtctgaaa tttctcaaat tctaaaaatc tcaaccaaac ttatctgata acactaaatt
    26041 cgaaagaatg cgtacaggta agtaacaatg aaaaaaattg ctattgtggg tgccgggcct
    26101 acggggatct acaccttatt ctcgcttcta cagcaacaaa ctccactttc tatttctatc
    26161 ttcgagcagg ctgacgaggc cggtgtcggg atgccataca gtgatgagga aaactcaaaa
    26221 atgatgctgg caaatattgc cagtattgaa ataccgccga ttaattgtac gtatctcgaa
    26281 tggctacaaa agcaagaagc cagccatctc cagcgttatg gcgttaaaaa agaaaccttg
    26341 cacgatcgtc agtttttacc gcgaattctg ctgggcgaat atttccgcga tcaattttta
    26401 cgattagtag accaggcacg acagcaaaaa tttgcagtgg ctgtttatga atcatgccag
    26461 gttaccgatc tgcaaattac aaatgctggc gtcatgctcg ctacaaatca ggatttaccc
    26521 agcgagacgt ttgatttagc ggtgattgcc acgggtcacg tctggcctga tgaagaagaa
    26581 gcaaccagaa cgtattttcc cagcccgtgg tcaggcctga tggaagcaaa ggtcgatgcg
    26641 tgtaacgtgg gtattatggg aacatccttg agcggactgg atgcggcaat ggcagtggct
    26701 attcagcatg gttcgttcat tgaagatgat aaacaacacg tcgtttttca ccgcgataac
    26761 gcaagtgaaa agctaaatat cacgttgatg tcgcgcacgg gtattttacc cgaagccgat
    26821 ttctattgcc ctattcccta cgagccctta cacatcgtca ccgatcaggc attaaatgct
    26881 gagattcaaa aaggcgaata tggccttttg gatcgggtat ttagattgat agtagaggaa
    26941 atcaagtttg ctgatccaga ctggagccaa cgcatagcct tagagagcct gaatgtcgat
    27001 tcctttgctc aagcctggtt tgccgagcgc aaacaacgcg acccatttga ctgggcagaa
    27061 aaaaatctcc aggaagtcga acgcaataaa cgagaaaaac atactgttcc ctggcgttat
    27121 gtcattctgc gcctgcatga agccgtacag gaaattgttc cacatctgaa tgaacacgac
    27181 cataaacggt tcagtaaagg ccttgcccgg gttttcatcg ataattatgc ggcaatccct
    27241 tcagagtcta ttcgtcggct gctggcctta cgtgaagcgg ggatcattca tattctcgcc
    27301 ctcggtgaag actacaaaat ggaaattaat gagtcgcgca ccgtcctgaa aacggaagac
    27361 aacagctact cgtttgacgt ttttattgat gcccgcggac agcgtccgct taaagtgaaa
    27421 gatattcctt tccctgggct acgcgagcaa ttacagaaaa caggggatga aatccctgat
    27481 gttggcgaag attatacgtt acagcaaccc gaagatattc gtggacgcgt agcgttcggc
    27541 gcgttgccct ggttgatgca cgaccagcct ttcgttcagg gacttacggc atgtgcagaa
    27601 attggtgagg cgatggctcg ggcggtcgta aagcctgcat cccgtgctcg tcggcgtctt
    27661 tcgtttgatt aaaggtaaag ctatcagcac tcaacaaact gatgctgttc attaagacga
    27721 tagcggactc cggttcgaat attgttttca ccttcaatgg cgacggcgaa gcgaactctt
    27781 tcgccgtcat gatatgccag tgcggcgctt ccccctggcc cgagaataat tgagtcgacc
    27841 acgcccgtgc tcactaccgt tgaattttca ccgctggcaa tgatgtgtac attgtcgccg
    27901 ctgttggcaa ttctggcgtt tgcgccaaag ctggcgattt gcaccaggtc accattactg
    27961 gcgacatgac accgttcacc caatgtacaa acgcgtactc gcattccggt attcgcaatc
    28021 cgactactat ctccagcgct gcttatccgc gaagaattac cggttacagc aacccgtgcg
    28081 cgttcaccgg agctgccaat gtgactgtta aagccgacac ttccaatccg cacgctataa
    28141 cccatactgg caatttgcgc ggcataccca gcggttacga ttcgggcgtt atcctcacaa
    28201 cagccgatgc gagcatgatc gtcgctgcgg gcaaactgac tgcaaaatgg tatctctccg
    28261 gggtctgtgg ccagtttttg catagaggaa acttcctgat gcgcgaagtt ttcatccgcc
    28321 agccattttt tccacgcata ctcaaccaga ctctccgccc agtccgtata tccttcctca
    28381 atcagtgcat gatgaatatc ggcataacta ccgccgcgtg ggaaatggcg caggaaccag
    28441 cgatacatca ccgctccgat acgccattct cgcaggtctg ctcgtgtaat gatcattcgt
    28501 tagtgccggt gatagccatc gaccatgctt ttaaaggttt cgtgtggcgt acgccccgtg
    28561 gtgcaaagat aaagatgtcc gaagataaaa aagaggctta taaatgccag agcaaaatgc
    28621 gcctgcaata accagtatct tacgccagga aacacatctc ccacggcttg cggatagaga
    28681 cacaacagcc ccgtcaatag taacaacggc agcaatccac acatgacacc aacataggcg
    28741 acctgctgta agggattaaa tttagactgt gttgttgccg ggaaaggatg ttcttccccc
    28801 tgcataatgc caaacaaata aaatcgtgtt tgttttgccg ctcgttccag ccacccctga
    28861 cgacgaatgc gatagtggtg accattatcc ccaacggcat tgatcagcac aaagccgagc
    28921 cagcatgcca gtaacaaaaa tccgcaaact tcatgcaccg caaccagact tttaaccgca
    28981 gttgcgccca ccatcgcaaa gtgatttatc agcccacttg ccagcaacaa tacaaagagt
    29041 aacgcattcg accagtgcca taatctgacc gctttgctgt aaagataaac cttctcacca
    29101 tgatcggtgg ctgatttctt cacgccacga gcacgaagca aggcgtgcag ccccaacacc
    29161 aaccacatcc caaccagcaa tactccggca atgatcaacc acaccggcca aaattcggga
    29221 gtgaactgtg gcacatagtt cgctaactgg ctctgaaact gttcagcatg ttgcgacggg
    29281 ttcatacatt ttccttttta atcaaatgtt gaccgaaccg gcgatacaga tgcggtttcc
    29341 cggcaccaag tagctgatat tgatagtatt tattttcctg caaccacgcc tgaatttctg
    29401 ggctatcttc gcgaccaaag atcaatgcgt gttccggaca ggcgctgacg caaatgggcg
    29461 gaaagccttt tgccagtcgg gactcagcgc agaaatcaca tttatctgcc actttagtga
    29521 cagggttaag gtagcgaacc tggtacggac aagcgccaat gcaataactg cagccgatgc
    29581 actgcgattt ttctacccgc acgatcccct gctcatcacg ccacgacgcc cctgtcgggc
    29641 atacgtcgat gcacggagca tcttcgcagt gctgacatga ctggcgaaaa aagtgatact
    29701 gcgtttcgtt gtcgttatcg gtgactggaa tgtgtgctat cgataaacgg cttccctgag
    29761 cggggacgtg gttagtttta cggcatgcgc gggcgcaaat attacagcca ttgcaccgtg
    29821 actcgtcatg aatcatggca taccgaactt ccttttcttg cctcgtgttc gctaacagag
    29881 aagacgcaga accagtaaaa aagattactg ttcccatccc cagaacaaat ttgcgtcgag
    29941 tgaaggacat ctgattatcc cctaatgcag tggctgcaac tgtccaccct atccatgaca
    30001 tctccctgaa atttcagggg ttctgaaact gctctacctc gaaccattcg cataagccat
    30061 aagccttgcc ataggtcata aatcgctcgc taaacaagta caacaccgcc gtcgatgact
    30121 ccacctgccg tatatcggca tattcaggtg caacatccat cgcggcgatc gcggcttcaa
    30181 tctgcgcttc ctggaagtaa tatggcgctt gcatcagcat ggcgactttg taaggacgag
    30241 gatacgtctg gcactcaaaa cgaacggcgt gtgcaatggc gcgacaaatg tcctgctcca
    30301 caacttgcaa agacatcgcc gcgtagtttt cactcatggc ctgcgttgag taatagtaat
    30361 catcctgact tcccgtcata caagcgatat ccgcgtagtc tccgttctgc ttaatgcttt
    30421 ccagcaaaac tgacagttcc tcttctgcaa ccgaataggg tggcgagaga aacacggtgc
    30481 gcgccaccag ctgcccagcc gcagaatgct ggcgaatgaa ctcagccagg atttcagcag
    30541 gttccggttc ggttaccgta tcaacggggt gacgcatccc ctgcaaccat tgccgcttcg
    30601 cctcgtcaac ttcgctgact ttcgctaacg gcaattcatc ctgatggttc atcttaccca
    30661 ttccttacgc aggcagtaga ttgtgtgctg ccagatcggc ggcaatatct tccagcccca
    30721 gacgctgcaa tgtttcgcgg gttggacaac caagctgtgg gtcccagccc atttctttgt
    30781 agaacatcgt gagcgaagca tgcatatcgt cacgatccat tttgtcagta ccttcagtaa
    30841 agaccgggat ctgcggatcc ttgtcgaaaa cccaggaaca gataagatcg tgttcattac
    30901 gcatatcttt ggtttgcatc agttttaccg tgtaggcacg atgcaacgta aagatacgct
    30961 ctgcagctaa atctaatttt tcctgagtca tctcttcgcc ggtgatcgct ttgaagaatt
    31021 tggcttcaag cgccagatcg ccccggtaat tacggctttt cagcggcgaa acggtcattg
    31081 gccagaccca gttgcacagg gtgacggcgt tatgcaaaca gacccgcaac agcgaccact
    31141 ttgcatattt aatttttgcg tcattgattg gcgtgtagtt tttggtttca tcgtaagcat
    31201 cttcagaacc aaaaagttct ttcgccactt cacgttgcag tttcaatggc aagccggaac
    31261 caataaagtt gatatgggta tgcgtcatgc agtcacggtt gaacatacag ttaacaatgg
    31321 aaccgacctg cgctgacgct tcattggcat ggtgaaccgg atagccaaac ggcgaccaga
    31381 gtttattttt cgcgtagccc cagtactctt cacccaaatt ccagcgttct gcgatggcat
    31441 atgaaccatc agccaggtga ctcagctcac ccacacgatg cgccagacgg tagtaaaaat
    31501 ctttaatgaa gttaacgtca cccgcttcca gttgatccca gcgaatttcc gcatactctt
    31561 cagcaggcag aacacgcttg aacacacctt tgctgtaaca ataggtaaag tcgcgatgca
    31621 actgcccgta gttacaccat aggccgtagt catcgaacag attcagaccc accaggttac
    31681 caatcacacg accatcgtct ttatcttcaa aatctttcgg accgttcggg aagatggtgg
    31741 tatggacaaa gttggctaca caagtgttac cacctgtgct gggcacgcca aactctttca
    31801 cccgaggaat attcatttgg gtcatacaac ggatcgggca agagtggcag ccactcattt
    31861 ttactgtgta tttctctgcc gccggaccta agtcaaaaac ggatttatag gtacgaaagc
    31921 cgaccgtgtt ctgattgcct ggcggaattt cccccgtttc aatcggacca ccttcagccg
    31981 cgccccaaaa cagcccttta cgtgctgtcc aacgtgactt gggatctgaa tactctgccc
    32041 acgattgcgg cgtacttggc acgacatggt tgttattcgc accaataagt tcagtcatca
    32101 tgtaatcgtt gagacgcttc atctcctgac gatcggcaat gttgacccct ttcgtccctt
    32161 caaccgcaat cgctttcagg tttttcgaac ccattattgc gccagttccc gcaccgccgc
    32221 tgtggttacg gctattcaac atgccagaga gaggaacaag gttttcccca gcctgaccaa
    32281 tagccgccac acaggtttcc ggactggtca atcgacaaat ttcttccgtc gttgcgcgcg
    32341 tcccttttcc ccataagaaa tcggcttttt ccaggctaac tttgtcatct ttaatcttca
    32401 gccataccgg tgatttcgcc ttcccttcga taataatgac gtcgtatcca gcgaatttca
    32461 tttgcgctgc aaaaaagcca cccatatggg catcgacgac taaatttcct ttggtaaaag
    32521 tagaaagtga ggtgatattt acgcgagaac tacagggggc accagatcca gttaatgggc
    32581 cggtagcaaa gactaattta ttcgcttcat cgaaaggttt cgtgcctggc ggtacttcgt
    32641 catacataat tttgtagccg aagcccatgc caccgacaaa acttttaaac ttactggaat
    32701 cttcgagggt aatatttcct gtcgtgagat tgactcttaa tatattacct gtccaaccgt
    32761 tagccatgat tttttccttt gcaagattca cacagtaata tctttccact cgataatttt
    32821 taacgcccct gttgggcagg cgtttgcgca ttcaccgcat aacacacatt tcgaggattt
    32881 tttactttcg gtatttacgg tggccatcat ccacgggcat gccgtagtac aggcgctaca
    32941 gccaatacag cgtttatgat cgacggtaat acagccttct ttctgctgcc aggtaatcgc
    33001 accaatcggg cagacgttca tgcattgcgg ctctttgcat tgacggcagg tgtccgcggt
    33061 atagttgaga tcgccataca ggccgccgcc agagccaacc ccgttgtcgc caaagaaata
    33121 attgcgatgg attttgatac gggagaagaa tgtccctact gagccatcgt tgaagttggt
    33181 acaagagatt tcacagcggt ggcaacccgt gcatcgcgct cgctgggtca ccagcacccc
    33241 tttgggtgta tttatcaacc ctactgtgcc gctatcaata tcttcctgtt tgcaacccaa
    33301 aagtgacagt aacgcagggg caattgttag accagcaagg ccttttcctg atatacgcaa
    33361 gaattctaat cgcgttaagc caatatctaa tagtggacga tcaaccgggt tcatttattt
    33421 cgcatcctct tcgcaaacac agtgctgaca attcagcagc ctgatattaa gcattcaact
    33481 aatacgctgc tgactctgtc cccaacacca gatattttcc ctaacccgat agtgtatgag
    33541 aaaagattta acgccgataa accaatcctt tatggttagg agaaaaaata atgtgattat
    33601 tctgagc
//
LOCUS       DAZPDU010000028        33487 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZPDU010000028 DAZPDU010000000
VERSION     DAZPDU010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33487
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.028"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..1013)
                     /gene="pilV"
                     /locus_tag="ACHURS_001223"
     CDS             complement(<1..1013)
                     /gene="pilV"
                     /locus_tag="ACHURS_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HFR1237004.1"
                     /translation="MKKTDKGVSLLEVLLVIGIMVMVIPKVYENIENHLNNVRWQNAA
                     EHANTYNTAVRNYVADNASTLLAGSLPKTITPATLIQKGYLKSGFSESNFGQSYITGI
                     AKNSKTSRLEALTCSNGGQSLSEAGMRSVASMIEGLGGYINSSKQAIGAGGGWSDTPS
                     NYGLNCATGHIAMALVGADLQESDRLYRYSITNRPDLNRMHTAIDMNSNNLNNVGTLN
                     GNAAALSGDISARNGTFSGAISGNTATTNGDITSNNGWLVTKNSKGWMNSTYGGGWYM
                     SDSSWLRSVNNKGIYTGGQVKGGTVRADGRLYTGEYLQLEKTATAGASCSPNGLVGRD
                     STGA"
     gene            complement(1026..1661)
                     /locus_tag="ACHURS_001224"
     CDS             complement(1026..1661)
                     /locus_tag="ACHURS_001224"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFR1237005.1"
                     /translation="MLNSIFNTAILCCANIVCQCYAYNEVKFRLNKLNVSYKTGTEKY
                     YCLILTTLAVLYLSLNQLSLIQSIIVIVFYAFLTLMACIDLLSFLLPRLYTVTFIFSG
                     LLYQTWNNNILSGLFCAMLMFFIMLFVRLYFAYKNGTESFGMGDVLLIAGTGVWFPTP
                     EIACSIVFIAVIGGIIFFTLGGLNKQKKYIPFGPFLCGGMFVYSLVPGILF"
     gene            complement(1665..2147)
                     /locus_tag="ACHURS_001225"
     CDS             complement(1665..2147)
                     /locus_tag="ACHURS_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFR1237006.1"
                     /translation="MRSVLYILLSCISSLIFSLQPAYSATTTRFDACFAQAGQRYLID
                     PLLLKSIATVESSLIPDRINHNKEKGKILSTDYGLMQINSSHIPNLKKLGVIKDKSEL
                     IDNPCLNIQIGAWILATHFQKCGINWSCLGSYNAGFKESNEQKRIKYARYVYNKYMVR
                     "
     gene            complement(2214..2771)
                     /locus_tag="ACHURS_001226"
     CDS             complement(2214..2771)
                     /locus_tag="ACHURS_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus major pilin"
                     /protein_id="HFR1237007.1"
                     /translation="MSSINILNMRSVFSSLSAHRKKEQDKGATLMEVLLVVGVIVVLA
                     ASAYKLYSMVQSNIQSSNEQNNVLTVIANMKSLKFQGRYTDSNYIKTLYAQGLLPSDM
                     IADTTGASAKNPWGGSVTITTSSDKYSFNVVEASVPKKNCMAMVNALRSSSAISKINN
                     TSTSTVDAATVCSSDSNTLTFSTDS"
     gene            complement(2816..3925)
                     /locus_tag="ACHURS_001227"
     CDS             complement(2816..3925)
                     /locus_tag="ACHURS_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HFR1237008.1"
                     /translation="MAIKDIFEKLNSIEIDFSGISHWLAKYTFRYSRISLYEDLSGML
                     NDGINVKRALTHLIDVETDYGKKTGSSATYYICCECLNALNNTGAISSALKKYIKEDE
                     YQLISSGEIRGDLAGGLVQAIKIVRSRSEMTMTLVMSMIYPSVLVFGCLANMYMVHDT
                     ITPVFLSLAPKERWTSSMRLLTDTSGFLIENGTYILCFCIVLFFLIRYSLARYTGPGR
                     QYLDYIPPWSLYKTFNGVSFLFNMASMLSIDIPVAKALERLEKNSTQNRWLLERIKEI
                     RRYVLLGQSLAMAMKSCGYDFPSKLCINKLLLHSENADNALMMSNYADKWLEEAKGKV
                     KSTGVWITVVSALIVFAFIVMMITALYDVSNVLQH"
     gene            complement(3916..5454)
                     /locus_tag="ACHURS_001228"
     CDS             complement(3916..5454)
                     /locus_tag="ACHURS_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HFR1237009.1"
                     /translation="MFFSDADYPGKDISDVVYVSISEKDDKPVQVYIADNSKGQRAVQ
                     AYIGTLNVKYPGQVNITSTTLDVIASRYQDSEKRSDSNSSQHLNSNQEKVISYLAKAN
                     KLGSSDLHITPGRDGSEFTYVEARVHGELEILDVIPRKEGLELLGAAYSGMSDVIKGT
                     QFDPAIPQDARIAENFLKPVNLFGARYSHYPCVGGVYAVFRLIKDDSEDIPTFEELGY
                     MPQQIQTIRRMLQRPEGIIVLSGPTGSGKSTTLRTASEAYLSTFGFNHNDNMRLPRKR
                     LFTIESPPEGRIPGAIQTAVRDSVDGWVDAIKSAMRLDPDAILNGEIRDHASALAAIK
                     ASMTGHLLLTTLHANDALNIIERLEMENIQARLIADPLLFIGLLSQRLVQKLCPYCKR
                     TYAEMADKLSTEERKLIEENCLPEQVRLRNLDGCEHCYKGVTGRTVIAEVVSPDARFF
                     QLYRQSGRLEAKSYWHYELGGITRNQHLLHLINHGLVDPLSAHFISPIDEDKYSMLPE
                     GTWR"
     gene            complement(5479..5973)
                     /gene="pilP"
                     /locus_tag="ACHURS_001229"
     CDS             complement(5479..5973)
                     /gene="pilP"
                     /locus_tag="ACHURS_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HFR1237010.1"
                     /translation="MLKIIKPTIISVCFFSSLAYATESESTPKDEIKYSSPTILQVEQ
                     IQAETVIYEAKLARQKAVSELRNLDLNGVASATPAITPSSGYSSLADTDIPAQRVAAK
                     SLRVIEIFGTPQQMSARISLLDGSVTDVKVGQSIPGTSYKVASITGNGVWIQNGTEKR
                     QLIL"
     gene            complement(5957..7267)
                     /gene="pilO2"
                     /locus_tag="ACHURS_001230"
     CDS             complement(5957..7267)
                     /gene="pilO2"
                     /locus_tag="ACHURS_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HFR1237011.1"
                     /translation="MSENNQNNRNFTSVIKNKRAFFSGLDWKTLPSEEKNARTFARKN
                     DAEYFLSCQYQDSENETKTMVAFIRKEDLPTGASSFWSLALMIKPLIEPDGYAICELG
                     DLYGFVSCVNNVLVNDVVGNKSQIMSALTTFLEFNETPDPGWKLYQPESWDISQALPS
                     LTLSALIDVKKPPKEAAFTRVSRKRQFMIYGGSAILAILLWNGITMYQEYREKEAAAE
                     AARIRLAKEMADKQAIQIAPPWQHLPEIKPFIDKCIDKWDALPLSIAGWRFDLAECST
                     SGNDGLLRTSYKELSGVTVEDFSTRIREIFQGTTTATFVLPEGSAGGFSLPVSFDVSP
                     DPITPDTLPQATDIQERLTTFAQKMRLKLTWQEIENTKTDEEGRPIILPWNEYELMIQ
                     TSTPPSILFANFHEPAVRFQYAGIKLEEGRLNYEIKGAFYVKNN"
     gene            complement(7318..8961)
                     /locus_tag="ACHURS_001231"
     CDS             complement(7318..8961)
                     /locus_tag="ACHURS_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HFR1237012.1"
                     /translation="MNKKLLCLLISSFLLSGCANFKKINANELAAERDAQTATEYAKK
                     IRNNPVIQDSTKQWINTTPIIEKQVKQSAPPCPIVINTRSDIELRQIAQRITQTCHIP
                     VRISADVWTYLNGTGTGSTQQMTGSIPAPDANGMVPLASVGSQPVKVSSGGTSLPELN
                     ISGLPALLQTVSSRLGISWKYDKGRITFFYLETRSFPITYMDSNVAYNSKVVSGTMSS
                     SGSTGSTSSGGMTGDASNTQTTTVEMKSSLYNDLKSEVSSMLTPGTGRMYLSTGSLTV
                     TDTPDVLDSVQEIVNKRNNEMSRQVVLNVEILSIKKSSQEQAGIDWNAVFNDGHLGLS
                     LGGSFGDAAENVITSGVSIVDGKLAGSKAFLKALSSQGSVSVVTQNSAVTKNLTPVPM
                     QIANQQGFIESVTTDSTANVGSSTSLNAATITTGFNMTLLPYIQPDSQNLQLLFSMSL
                     SDKPTFEVFESGGSKAQTPNVDLKTINQTIDLKSGQTVILSGFQQSNRKTSKQGVITP
                     SFFGLGGGVNSEDGDTILVVLITPNVLNSGSSAVIKNVN"
     gene            complement(9012..10970)
                     /locus_tag="ACHURS_001232"
     CDS             complement(9012..10970)
                     /locus_tag="ACHURS_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HFR1237013.1"
                     /translation="MDAKKTGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLK
                     WDTFHSVISTFSGNPQYKKLVFMAWFGFSVPLIFFAIFMLIVVIGIMPKKVIYGDARL
                     ATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQFIYFAGQQFLILYAPTRSGKG
                     VGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQECFLFAPAGYAETIDQAIKG
                     QIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDK
                     ERFHLEQKAKGHNVPDVLVSISAILKTSVPDGGKDLAAWMGQEIENRSWISDKTKSFF
                     FKFMSAPDRTRGSIETNFSSPLSIFSNPITAEATNFSDFDIRDIRKKPMSIYLGLTPD
                     ALITHEKIVNLFFSLLVNENCRELPEHNPDLKYQCLILLDEFTSMGKSEVIERAVGFT
                     AGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLYYPPKSKNALAKKISEEIG
                     VRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNIAIREII
                     TSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKA
                     GGKKVMVAGGNVITNPDLDNHDKTDVSE"
     gene            complement(10986..12041)
                     /gene="virB11"
                     /locus_tag="ACHURS_001233"
     CDS             complement(10986..12041)
                     /gene="virB11"
                     /locus_tag="ACHURS_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001708009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043684 - type IV secretion system
                     complex [Evidence IEA];
                     GO_process: GO:0044097 - secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="HFR1237014.1"
                     /translation="MNNIRDTSNTSQPRDNSKTARRYLDMTGIQSVLNIEHVTEISVN
                     KPGTIWFEGKNGWESKDAPDATFDNLMTLAKTLTNLSKIKIPLSHDNPIASVVLPGGE
                     RGQIIIPPATENNSVVISIRKPSLTRFTIDDYVRTGRFDNVRIATKHEAILTERQRYL
                     YELSRRPDGQSKAQFLREAVKDRLNFLIVGGTGSGKTTIAKAIADIFPPERRYITVED
                     VPEMSLPLHPNHIRLFYKKNTVEAKEIIEACMRLKPDHIFLAELRGNEAWSYLEALNT
                     GHEGSISTIHANNTYASFSRLASIVKQSDVGMTVDMDLIMRTIKTSIDVILFFNHTRM
                     TELYYEPEEKNRFLSTM"
     gene            complement(12084..13223)
                     /locus_tag="ACHURS_001234"
     CDS             complement(12084..13223)
                     /locus_tag="ACHURS_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HFR1237015.1"
                     /translation="MKNKDDEKNNDAGNRGIIEVKGKAAPKKILILIILLIAALFVII
                     ILFKVLSREQVVQQTPLEKSDETLVTNTNNGVSLTTMMKNIEEKEKLDAANRRKAQEE
                     QEQKQEDNAPSAPASQKADQTAVNVIANGTPQTASGDPNQPQPLPKSVRQLMGDTMVK
                     IDNQEPGEKNQERDDLQGSQYADGKVSPVLNRRYLLSAGTALSCVLKTKIVTSYPGIT
                     MCQLTRDVWSDNGEVLLARKGALLIGEQNKVMTQGVARVFVNWTTLKDENVNVRIGAL
                     GTDSLGASGLPAWVDNHFGQRFGGALLLSLLGDGLDILKNSTQQTGSNSNITYENTSD
                     ATKEMAKTTLDNTINIPPTAYINQGTVLSVIVPRNIDFSSVYELQ"
     gene            complement(13213..13914)
                     /locus_tag="ACHURS_001235"
     CDS             complement(13213..13914)
                     /locus_tag="ACHURS_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbG/VirB9 family P-type conjugative transfer
                     protein"
                     /protein_id="HFR1237016.1"
                     /translation="MYGTPSERDGRIQTVDYNEQDVFNVRVKAGAQTTIKFGQDETIK
                     DVGIGDPEAWSVSVRDNTLFLRPKAEEPDTNVTVQTNKHIYPLYLISTTKQPTYILRF
                     NYPKPPSATVMTEKPFPCTDGGIINGHYQLKGDKSIFPYQVWDNGEFTCMRWTNKQEI
                     PVLYRVDADGNEHLVNGDRNKNTMVYYDVAENLRLRLGDQVADIRTSSIVNRPWNKKG
                     TSNGKTRVEKFSYEK"
     gene            complement(13980..14714)
                     /locus_tag="ACHURS_001236"
     CDS             complement(13980..14714)
                     /locus_tag="ACHURS_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virB8 family protein"
                     /protein_id="HFR1237017.1"
                     /translation="MENTNYYEHSKKEASKKKFEEKNEKKDYFKAIRDFERSEIEIIK
                     KKAKTFTILAIGEFVVICILGFAIASLAPLKTAVPFLVRVDNSTGYTDIAPQLSDAKE
                     SYQDVETKYFLSKYLINYEAYDWQTIQEQADTVKTMSSQKVFSAYDTMIRADSSPLNI
                     LKNNYKIKVQINSVILLRKDMAQVRFKKMVLDLSGKPAPGYRATEWISTISFDWDKDI
                     KTEKERLVNPLGLQVLSYQPDPEVIK"
     gene            complement(14880..17237)
                     /locus_tag="ACHURS_001237"
     CDS             complement(14880..17237)
                     /locus_tag="ACHURS_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion system protein"
                     /protein_id="HFR1237018.1"
                     /translation="MHKIKLKNSDNMDVAREYPDYRFHITDSIIFTSDRKMLASLVVA
                     GIPFETENDNVLTNLFNSVKNFLIGLGKEGDLYLWTHLIKKRITIDGEWNFDNAFLSR
                     FSKKYLALFTSSAFYKSTWYLTFGIPYDDVDTGIERMNEMTQQAQKALQPFNASVLSV
                     HNTYISEVADYLSLLLNTEHNMIPLSSTPLSSSICDSEWYFGADVLELRNNESDSKKF
                     ATNYILKDFPIETTPGQWDFLLKQPYEFILTQSFIFESPTKTLKNIDSQLNKLQSAND
                     AAKTQQEELEAGKEAVAAGITLFGSLHCALTVFGDTPDQARSNGIKLSAEFITSGKGF
                     RFSRASLASPFVFFSHMPLNKRRPLDTRRTITNLACLMSFHNYSSGKKSGNPIGDGSA
                     IMPLKTVSDSIYWFNTHYSPPEKNVTGQKIAGHGMILGATGTGKTTFEAAASGFLQRF
                     DPLMFVVDFNRSTELFVRAYGGSYFTLQEGIYTGCNPWQLAAGPESPVWHRLLAFLKR
                     WTQVLARDNQGNPCSDEHGIELNAAVESIMRMPVEERRTALLLDIVSPELQTRLAKWC
                     DNGEYAWAVDSPRNTFNPLHHKKVGFDTTVVLDTKNGIHPACEPLLAVLFFYKEIMQR
                     GGNLMLSIIEEFWMPANFPMTQSMIKSALKAGRMKGEMMWLTSQSPEDAINCAIFAAL
                     VQQTATKILLPNPDAKWEGYKEIGLTEKEFEKLKELTKESRTMLIKQSGSSVFAKMDL
                     FGFDEFIPVLSGSETGLSIFDEIIAEKGDVAPDIWIPELLKRLNG"
     gene            complement(17243..17563)
                     /locus_tag="ACHURS_001238"
     CDS             complement(17243..17563)
                     /locus_tag="ACHURS_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="HFR1237019.1"
                     /translation="MDKVNEHVSESFMTYNGFNRPALIAGIPLMLLLFTAFFAVLTGF
                     PAIFLWGIKGIIIPVICALFLFIVKLACENDSNALRVIRLNLMGLLLKIRHRDLIIGY
                     SSVR"
     gene            complement(17634..17924)
                     /locus_tag="ACHURS_001239"
     CDS             complement(17634..17924)
                     /locus_tag="ACHURS_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001708011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFR1237020.1"
                     /translation="MKVKSTLQYFILAFFLSLTASVAYAGGLDTATNTMTELKTWAFS
                     FGGVCALCYLIYNVIMAFMEKKSWSDVGMALVYCSLAGGALYAGNWALELFK"
     gene            complement(17924..18508)
                     /locus_tag="ACHURS_001240"
     CDS             complement(17924..18508)
                     /locus_tag="ACHURS_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFR1237021.1"
                     /translation="MQLSAAVLAALISQCAPDVSPDTMNALIMTESGANPYVIANVSD
                     GTSKYFKDEKGAIEYAEKLTAENKRFSAGLTQIYSKNFPSLNLTNKTVFDPCTNIKAG
                     AAVLTDNYLRQKEGSSNQKILRALSLYYSGNESTGFIKEKKFNNTSYLERVIRNANNY
                     IVPSIREKTDESEINPPNDEEKTSPDWDVFGDFS"
     gene            complement(18529..18927)
                     /locus_tag="ACHURS_001241"
     CDS             complement(18529..18927)
                     /locus_tag="ACHURS_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1237022.1"
                     /translation="MQDNDDVSELDIATREVLILRCNLIQLASEYIKINPLKNTRIYT
                     DLIHRSADIIKNIEQGNIQNKHNYLSQDVKHTLVLYDLYKSDFEQCPGAKSLIPVREK
                     IVSLKELLSGANELDSIVRRPRRKKRSSRI"
     gene            complement(19046..19483)
                     /gene="pilM"
                     /locus_tag="ACHURS_001242"
     CDS             complement(19046..19483)
                     /gene="pilM"
                     /locus_tag="ACHURS_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HFR1237023.1"
                     /translation="MGWFFSLLLIFLSIGSYYLTDNNNQTIQRMKLLPAKQEAIEFVH
                     FANVINDYLYKYPDKRNSGGTLTSEQIGITPVYDIHHIIYGKRVYIWSADTEGLMSAL
                     QQQTKHSAMLGRVKNKKIVDNQGNDMGVPVPSSIPEEAIVLIN"
     gene            complement(19489..20724)
                     /locus_tag="ACHURS_001243"
     CDS             complement(19489..20724)
                     /locus_tag="ACHURS_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpQ domain-containing protein"
                     /protein_id="HFR1237024.1"
                     /translation="MKKLIIIPTTLLLNACSHAPEPAQAKGGWYELNTTIEQVRADTY
                     YNGVILQQPVSQPNSTVQYSNDNYLKKEAINVTPAPRIAETVKPRSEQSATAPKNQGS
                     NNSFLKPTTNSITSSGEIKTAAKGATKQHSEQPALSPAKPISSNNHQTQTAQKTDQQT
                     KPRPEQPPLMSKSGFLKKNANNAVPATTLTGPSTPEKQIYFVRHDGKGSLQKALKEIV
                     PSDWTAEISPEVTKTFRRTISWQGNDQWPYVLDKMLRNYGLTVIKNTEKRHLLIDFQQ
                     QKGKTSQKAPVSPDKTPASPVRTPVSSDKLPQKSLTPVTVPQKQTEKLTWNLKKGSTL
                     RDGLTEWASSSPCGNSTWNLVWDTPVNYRIDAPLIFRGNFESALKDTLMLYLSADKPL
                     YAFRNIAQCVITIKDTQQE"
     gene            complement(20727..21026)
                     /locus_tag="ACHURS_001244"
     CDS             complement(20727..21026)
                     /locus_tag="ACHURS_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbM/KikA/MpfK family conjugal transfer protein"
                     /protein_id="HFR1237025.1"
                     /translation="MKSIILSMVAASAIALSAPVYASDPCASVLCLYGKAIGSGGGSE
                     CKSAEKDFFNIIKKKKGSIRWSKTFDARKAFLNQCTTADPAAISKIMSKFGRSWG"
     gene            complement(21093..21728)
                     /locus_tag="ACHURS_001245"
     CDS             complement(21093..21728)
                     /locus_tag="ACHURS_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1237026.1"
                     /translation="MKSIILSMVAATALLSGCADSKTQPVPESPLTSNDHQITLKIDT
                     VRTDLPDSSPVCLNGMNIYESLSERHPGLFAKNQNVIFRSAFFDDGQAISSETIRKIM
                     QSTPCVESQTTSAGSWEPATELIPRQAISGDTTLAEKDRTAGFTMRIAPEIERGNFHL
                     LLTTTDPIRKQTVEQNILLKPDQPLIVSGFTGVNADSQSENRIVIITPHVR"
     gene            complement(21881..22135)
                     /locus_tag="ACHURS_001246"
     CDS             complement(21881..22135)
                     /locus_tag="ACHURS_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="HFR1237027.1"
                     /translation="MNKSRNAIIASSIIFASLMLVGCKEKIYSVEYYSNNISEATKTL
                     EDCKKGTITDQNCDNARAALQQKQDSEYKKKVSEMRRRLD"
     gene            complement(22137..22778)
                     /locus_tag="ACHURS_001247"
     CDS             complement(22137..22778)
                     /locus_tag="ACHURS_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001425343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HFR1237028.1"
                     /translation="MKDKVLSFLFAGIITITSPSIFASGIPVVDVAAIAKTVEEGLNR
                     AAEAARQLEQLKQQYEQTIRYAEEQKKRLEGFTDFSNGFDSASSYMKDSLSTITNSAK
                     SDLSSLRSQYDLSSNVAETQQKYDAILAKIKFYENFNNEMQERAKRLTTLQKEFASAD
                     TPQKKADLSNQLNTEKLTMELQLKQYDIAERQLENERKAQYETYVRQKRRDLS"
     gene            complement(22784..23779)
                     /locus_tag="ACHURS_001248"
     CDS             complement(22784..23779)
                     /locus_tag="ACHURS_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030255 - protein secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HFR1237029.1"
                     /translation="MNIVSEFLEKVTTIVESTAATNAAKVAQAISPTFFAAIALYVIY
                     LIYEITYAQRDVLMSEVTKNIGAFALVGAFTYSAPYYSQFVIPFVMHAGSDLSAAVTG
                     GSGTATSVDNLWNMLSVTLNDFKNNELDTLEWYSFTDQLYIYLIWGVGYVGGLLLIYY
                     TTVFLTLSTFMVGILLSAGILFICFSLFASTRNMFSAWVGSCLNYILLNLFYSISFSF
                     VISFVEQTVPSSGNITLTTVVYFFMVTVISIFLVEQVGTLCSTLTGGVGINGLTSAAN
                     GFGGKVASGFMRASGLRSFTNGFASKMPNPFFNAGSRSASALVQGLNRGSKLKAG"
     gene            complement(23783..24040)
                     /locus_tag="ACHURS_001249"
     CDS             complement(23783..24040)
                     /locus_tag="ACHURS_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1237030.1"
                     /translation="MKKNDDLITLPSHNSHALDYIIKKAFTPRKTKTSTATHNVYSYN
                     KEESTLNFQKIGEAIFFGTDLVIKLKPNISASGELFICQKD"
     gene            complement(24037..24339)
                     /locus_tag="ACHURS_001250"
     CDS             complement(24037..24339)
                     /locus_tag="ACHURS_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1237031.1"
                     /translation="MTQAKANLRKLRRARYVPPRTVKKNIANTERVVSKAITGCGVFR
                     DIPQVKINLIETRKILQIELALSIANLSEANPIEIRKKIKSIDRQLSSTSKIRTTL"
     gene            complement(24602..25258)
                     /locus_tag="ACHURS_001251"
     CDS             complement(24602..25258)
                     /locus_tag="ACHURS_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1237032.1"
                     /translation="MRNANEGISLAAIVAKETTTGKWNGTGGGIGLGTGGIGLFIGGV
                     SGSKQEQTQRAKNFESPKEEEYSLLNVYGSIIKVLVFGVLFGLSARLFTFLSDTGSDL
                     AEPVTTTTATGKIQSILGGMAEMVSILGTVVPFLVIIIAMIWFLFFSSKREKEELTRV
                     EREAAKTKLRIDVYHRLRYVESDHVVFDPITGREVPAERACINKLIEALADESNNVRG
                     "
     gene            complement(25443..25886)
                     /locus_tag="ACHURS_001252"
     CDS             complement(25443..25886)
                     /locus_tag="ACHURS_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFR1237033.1"
                     /translation="MLWFTLFLLCIALEIITGTGWLLLISLGALSSAIVGFFLPISQE
                     ANICFFASISILASIIKFFYDKKHKKLDTLLVNTGHSRFKGKEFTLSDDIVNGKGQLL
                     IGDTFWPVETLHDENYPAGTRVIVTDMQGITLKVMTIEDKSESKE"
     gene            complement(25949..26902)
                     /locus_tag="ACHURS_001253"
     CDS             complement(25949..26902)
                     /locus_tag="ACHURS_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFR1237034.1"
                     /translation="MVTSIPLLLLILVALIFVKSAVKIVPQGNAWTVERFGKYTHTLS
                     PGLHFLIPFMDRIGQRINMMETVLDIPKQEVISKDNANVTIDAVCFVQVIDAAKAAYE
                     VDNLASAISNLVMTNIRTVVGGMNLDDMLSQRDSINSKLLTVVDYATDPWGIKVTRIE
                     IRDVKPPKELTEAMNAQMKAERTKRARILEAEGIRQSEILKAEGEKQSQILKAEGERQ
                     SAFLQSEARERQAEAEARATKLVSDAIAEGDVQSVNYFIAQKYTEALQAIGTASNSKL
                     VMMPLDSSSLVSSVAGISELLKNVSQDTPKVDMKQAQQYLS"
     gene            complement(26948..27142)
                     /locus_tag="ACHURS_001254"
     CDS             complement(26948..27142)
                     /locus_tag="ACHURS_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFR1237035.1"
                     /translation="MPKYQINATIKKPGGGPVKWTHYSDFPMTAEECRKNFSKEKEAG
                     KCFSVDVHISDFICKEIHPQ"
     gene            complement(27135..27587)
                     /locus_tag="ACHURS_001255"
     CDS             complement(27135..27587)
                     /locus_tag="ACHURS_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiF/GrlA family transcriptional regulator"
                     /protein_id="HFR1237036.1"
                     /translation="MARKLKINPVIPECVADLKGYPLYIIVAYWGLRNNKTLTTTDVS
                     RNFLITQQQSYDVLTYIYQEAQKQITATKRTLFNEHKRRISAFRILDIDKGILRTPKK
                     EIAYASYDNIPTVTMKSTRCEIQDSFTELRRWMCSRKTGDRVEVFLNA"
     gene            28719..29747
                     /gene="repA"
                     /locus_tag="ACHURS_001256"
     CDS             28719..29747
                     /gene="repA"
                     /locus_tag="ACHURS_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001175215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiator RepA"
                     /protein_id="HFR1237037.1"
                     /translation="MQLAVISKRHWSELSPEEQVRFWQDYEAGIESSFLVPQENKGGT
                     TKRRRGEHSTKPKCENPAWFRPDNYKALGGQLGHAYNRLVKKDPVTGQNTLRMHMSLH
                     PFYVLKRQSVGRKYKFRPEKQRLLDAIWVVLVSFCDRGLHTVGMSVSRLAEEISPKDS
                     KGDVIPETAVTVSRLSRLLAEQVSFGTLGTSEKTIWDRESRQRLPKYVWITETGWKML
                     GVDLVKLQEQQRKRLAESEIRLQLIKEGVIREGEEISVHSARKRWYAQRSLDAIKSRR
                     KKAAERKRANRLAKLPYDEQRNEIARFILKRMPPDEAYWCTKERLEQLVARDLRQLEL
                     ALTASPPH"
     gene            complement(29845..30216)
                     /locus_tag="ACHURS_001257"
     CDS             complement(29845..30216)
                     /locus_tag="ACHURS_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1237038.1"
                     /translation="MAEYLQRFFVGGLEGLCFSLGRSPYARREAVGGTFNILSGAALA
                     AASEGIFVIIHSCFKDKYFPCGEATQGIKEQGLRIYCGFDVLCLLLSEVIPLRVFVDN
                     RRRFYSDTLLRPSKNCQSGTK"
     gene            31098..31250
                     /locus_tag="ACHURS_001258"
                     /pseudo
     CDS             31098..31250
                     /locus_tag="ACHURS_001258"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5431 family protein"
     gene            31195..31317
                     /locus_tag="ACHURS_001259"
     CDS             31195..31317
                     /locus_tag="ACHURS_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004118124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR1237039.1"
                     /translation="MVCCTLLIFTYLTRNRLCEVRLKDGNREVTASLAYESNGK"
     gene            31467..32063
                     /locus_tag="ACHURS_001260"
     CDS             31467..32063
                     /locus_tag="ACHURS_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFR1237040.1"
                     /translation="MTAIIEASMQPKKTPVIVVKKRRVLVMPENPVVNEKPQEVQKSA
                     VNENKKVQKKDAVAEKTHKKQPQPWYLKKQITFPPKYPKEYFEKCFNKVRAVFPELWT
                     DEKKNLPLKSGILQDVEKYLADNPDVDLTIEEWNCAVQVMTFRWQYLQNCTVPGATRY
                     DLYGKPAGTVKKAHATYAQLVLDARKKASEKKQLKRKG"
     gene            complement(32081..32365)
                     /locus_tag="ACHURS_001261"
     CDS             complement(32081..32365)
                     /locus_tag="ACHURS_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFR1237041.1"
                     /translation="MALDLKTPVIPGQKTEQTNADTARFINEATRKKSGATKVTNVRL
                     GDVYEDILEREALRTGQTKTAIMKAALAAYEHGLDENGKNHWILQSARLK"
     gene            complement(32439..33104)
                     /locus_tag="ACHURS_001262"
     CDS             complement(32439..33104)
                     /locus_tag="ACHURS_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="HFR1237042.1"
                     /translation="MGRALLVASDKGGVGKSTTATNLASYLVNNSRTVIILKTDKNND
                     ILSWNEKRKQNGLLPVPVYEEYGDVSEVIKRLKKICEVLIIDCPGHDSKEFRSALTVA
                     DILLTLVKPSSDFEAETLTHVTEKVRTAQQINPDLQPWVLFTRINTTKPRHRKAAIDL
                     DKLLRENPVWIQPLRTRISDLDIFEAACNEGAGVHDVRRASSLSTAKAQIELLAQELG
                     INP"
BASE COUNT         8925 a         7091 c         7309 g        10162 t
ORIGIN      
        1 gcccctgtac tgtcgcggcc tacaagcccg ttaggggagc aggaagcgcc agccgttgct
       61 gttttttcaa gctgtaaata ctcgcctgta tagaggcgtc catcagcgcg tacagttcca
      121 ccttttacct ggccaccagt gtaaatccct ttattattca cgctgcgcaa ccaggacgaa
      181 tcggacatat accagccgcc accataggtg ctgttcatcc agcctttgct gttttttgtg
      241 accagccagc cattattcga agtgatatca ccgttggttg tggctgtatt tcctgaaatt
      301 gcgccagaga acgtaccgtt acgtgcgctg atatcgccgg agagcgcggc ggcgttgccg
      361 tttaaagtgc cgacgttgtt cagattgttg ctgttcatat caatcgccgt atgcatcctg
      421 ttaaggtcgg gacgattagt tatggagtaa cgatacaaac ggtcgctttc ctgcaaatca
      481 gcgccaacaa gtgccattgc aatatgcccg gtcgcgcagt tcagcccata gtttgaaggc
      541 gtgtcagacc agccgccacc cgcgccaatc gcctgcttgc tgctgttgat gtagccgcca
      601 agcccttcaa tcatgcttgc taccgagcgc attccggctt ctgacaggga ctgaccgcca
      661 ttactacagg tcagggcttc cagacgggat gttttgctgt tctttgcaat accggtaatg
      721 tagctttgtc caaaattgga ttcagaaaaa ccagatttca gatagccttt ctgaatgagt
      781 gttgctggcg tgattgtttt gggtaaagag ccagcaagta gtgttgatgc attgtcagca
      841 acataattac gaacagcggt gttataggta tttgcgtgtt ctgctgcatt ctgccagcga
      901 acattattca gatggttttc aatgttttca taaactttag ggataaccat caccattatg
      961 ccgatgacaa gtaatacctc aaggagagat acacctttat ctgtcttttt cattgctctt
     1021 aatcctcaga ataatattcc cggtacgagt gagtaaacaa acattccgcc acaaaggaac
     1081 ggaccgaatg ggatatattt tttttgcttg tttaatccgc caagagtgaa gaaaataatt
     1141 ccgccgataa cggcaataaa tacaattgaa caggcaatct caggcgttgg aaaccatacg
     1201 ccagttcctg caataagtag cacatctccc atgccgaaac tttccgttcc gtttttatat
     1261 gcaaagtaaa gtcttacaaa cagcataatg aagaacatca gcattgcaca aaacaaacca
     1321 gacaatatat tgttattcca tgtctgatat aacaggccgg agaagataaa tgtcacggta
     1381 taaaggcgtg gtagtagaaa tgaaagaagg tctatacagg ccattaatgt aaggaaagca
     1441 tagaaaacaa tcactattat ggattgaatc agtgatagct ggtttaatga aagatacaga
     1501 acggcaagcg ttgtcagtat cagacagtaa tatttctctg tgccggtttt ataagataca
     1561 ttaagtttat ttaaacggaa ttttacttca ttgtaggcgt agcattgaca gacgatattt
     1621 gcacagcaaa gtatcgccgt attaaatatg ctgtttagca taagttacct taccatgtat
     1681 ttattgtaga catagcgtgc gtatttgatt cttttctgtt cgttactttc tttgaaacca
     1741 gcgttataac tgccaagaca actccagttt atgccacatt tctgaaaatg tgttgccaga
     1801 atccacgcgc caatttgtat attgagacag gggttatcaa taagctctga tttgtctttt
     1861 attacgccaa gttttttcag gttggggatg tgtgatgaat taatttgcat cagtccgtaa
     1921 tcggtgctta atatttttcc tttttctttg ttgtgattta tccggtctgg tatgagtgaa
     1981 ctttctacag tggcaatgct tttcagaagc aggggatcaa tcagataacg ttgcccggct
     2041 tgtgcaaagc aggcatcaaa tcgggtcgtt gtcgctgaat atgcgggttg caatgaaaag
     2101 attaatgatg aaatacatga tagcaggata taaagaacag aacgcacaat actcctcctg
     2161 taaacatgga aaaaataagc gcccgaaggc gcttatccga tgcacatgaa aaatcaggaa
     2221 tcagtgctga aggtcagcgt attgctgtca gatgagcaaa cagttgccgc atcgacagtt
     2281 gatgtggaag tgttgttaat cttggagatt gcgctggaag aacgcagggc gtttaccatt
     2341 gccatgcagt ttttcttcgg tacgctggcc tcaacaacat tgaaggagta tttatccgaa
     2401 gaggttgtga ttgtaacgga gccgccccac gggtttttcg cgcttgcgcc agttgtatct
     2461 gcgatcatat cggatggcag taatccttgc gcgtagagag tcttaatata gttactgtca
     2521 gtataacgcc cctggaattt cagtgatttc atgttggcaa tcactgtcag gacattgttt
     2581 tgttcattgg atgattgaat gttggattgc accattgaat agagtttata tgcacttgct
     2641 gcaagtacaa caataacacc aacaaccaat aacacttcca tcagtgttgc ccctttgtcc
     2701 tgttcttttt tacggtgtgc agaaagagaa gaaaaaacag aacgcatatt taaaatatta
     2761 atagaagaca taataaataa ttccttattt attgagaggt taaaaacatt agggattagt
     2821 gttgcaggac gttactgaca tcgtagagag cagtaatcat cattacaata aatgcgaaaa
     2881 caattaatgc ggatactaca gttatccaga ctcctgttga tttgactttt ccttttgctt
     2941 cctccagcca tttatcagca tagttagaca tcatcaatgc attgtcagca ttttcagaat
     3001 gaagtaatag tttgttaatg cataatttcg atgggaagtc ataaccgcaa cttttcattg
     3061 ccattgcaag tgactgtcca agtaaaacat atctcctgat ttcttttatt cgttcgagta
     3121 gccatctgtt ttgagtgctg tttttctcca gcctttccag tgcttttgca acaggaatat
     3181 caatacttaa catggaggcc atgttgaaga gaaaacttac accattaaat gttttataaa
     3241 gtgaccaggg gggaatgtaa tcaagatact gacggcccgg tcctgtataa cgtgcaagcg
     3301 aatatctgat caggaaaaac agtacgatgc agaagcagag aatatacgtt ccgttttcta
     3361 tcaggaaacc agacgtatct gtcagtaatc gcatagaact tgtccagcgt tctttcggtg
     3421 cgagagaaag gaaaacaggt gttatggtat cgtggaccat atacatgttt gccagacaac
     3481 caaaaaccag tacggatgga taaatcatcg acataacaag agtcattgtc atttcagaac
     3541 gacttctgac aatttttatg gcctgaacta atcccccggc aaggtcgccg cgaatttcac
     3601 cgctggatat taattgatat tcatcctcct ttatatattt ttttaatgct gaagatattg
     3661 cccctgtgtt attcagtgca ttaaggcact cacaacaaat atagtaggtt gcagaagacc
     3721 ccgttttctt tccgtagtct gtttctacat caatcaggtg tgtaagagca cgctttacat
     3781 ttatgccatc atttaacatt ccagataaat cttcatataa acttattctg gaatacctga
     3841 atgtgtattt tgccagccag tgggatattc cagagaaatc aatttctatc gaattgagtt
     3901 tctcaaatat atcttttatc gccatgtccc ctccgggagc attgagtatt tatcttcgtc
     3961 aatgggggat atgaagtgtg cggacagcgg atctaccaga ccatgattaa tcaggtgtaa
     4021 taagtgctga ttacgggtaa ttcctccaag ttcatagtgc cagtatgatt ttgcttccag
     4081 acgcccggac tgacgatata actgaaaaaa acgtgcgtcg ggtgagacga cttctgctat
     4141 aaccgtcctt cccgttacgc ctttataaca gtgctcgcaa ccgtcaagat tgcgcaaccg
     4201 gacctgttcc ggcaggcaat tttcctcaat gagtttccgt tcttctgtac tcagtttgtc
     4261 tgccatttca gcataagtgc gcttacagta aggacagagt ttttgtacca gccgttgact
     4321 cagcagaccg ataaacagaa gaggatctgc aatcagcctg gcctgaatat tctccatttc
     4381 aaggcgctca ataatattca gggcatcgtt ggcgtgtaag gtcgtcaaca gaaggtggcc
     4441 tgtcatcgag gctttgattg ctgctaatgc cgatgcgtga tcacgaattt cgccgttcag
     4501 tatcgcgtct ggatcaagtc gcatagcgga tttaatcgcg tcaacccagc catcgacgga
     4561 atccctgact gccgtctgta ttgcgccggg aatgcggccc tccggcggtg attcaatggt
     4621 gaaaaggcgt tttcggggga gacgcatatt atcgttatgg ttgaaaccaa acgtgctcag
     4681 ataggcttct gaagccgttc tcagagtcgt tgacttaccg gaccctgtag ggccggataa
     4741 aacgataatt ccttccgggc gttgtaacat tcgcctgatt gtctggattt gttgcggcat
     4801 gtagccaagt tcttcgaaag tgggaatatc ctcgctgtca tctttaatca ggcggaagac
     4861 cgcgtacaca ccacccacgc acggataatg cgaatagcgc gccccaaaaa gatttacagg
     4921 cttcagaaaa ttctctgcga tacgtgcatc ctgtggtatt gcagggtcaa actgcgtacc
     4981 tttgataaca tcactcattc cagaataggc agcacccagc aattccagcc cttctttacg
     5041 cgggatgaca tccagaattt ccagttcccc atgcacgcgt gcttcgacat aagtgaactc
     5101 ggagccgtcc cgaccaggtg tgatgtgcaa atcggaactt cccagtttgt ttgctttcgc
     5161 caggtagcta atgacctttt cctggttgct gtttaagtgt tgtgatgagt ttgaatcaga
     5221 ccttttttcg ctgtcctggt agcgggatgc gataacatca agagttgtcg aggttatatt
     5281 tacctgtccg ggatatttaa cattcagagt gccaatataa gcctgaacag cacgctgtcc
     5341 tttactgtta tcagcgatat aaacctgaac aggtttatcg tctttttcag atatactgac
     5401 gtatacaaca tcagaaatat cttttccggg atagtctgcg tcagaaaaaa acattatgtg
     5461 tcgtctcttt caatagcatt aaagaataag ttggcgtttc tctgtaccat tctgaatcca
     5521 gacgccatta ccagtgattg atgccacctt ataagaagta ccaggaatac tttgcccgac
     5581 cttgacatca gttactgacc cgtccaggag agaaattcgt gctgacattt gttgtggtgt
     5641 accaaatatt tcaataacac gaagtgattt agcggctaca cgttgcgctg gtatatcggt
     5701 atctgcaaga gaagaatacc cggaagaggg cgttattgct ggcgttgcgg acgctacacc
     5761 attcaaatca agattacgca attcactgac agccttctga cgcgccagct ttgcttcgta
     5821 aattaccgtt tcagcctgaa tctgctctac ctgtaaaatg gtcggagatg aatattttat
     5881 ttcgtctttt ggtgtgcttt cagattctgt tgcgtaagca agtgaactga aaaaacagac
     5941 agaaataatc gttggtttaa ttatttttaa catagaatgc acctttaatc tcataattca
     6001 gtcttccttc ttccagtttt attcctgcat actggaagcg aacggcaggt tcatgaaagt
     6061 tagcgaaaag tattgaaggc ggggtgctgg tctgaatcat taattcatac tcgttccacg
     6121 ggagaattat tggacggcct tcttcatctg tttttgtgtt ttcaatttcc tgccaggtga
     6181 gttttaacct cattttttgt gcaaatgttg tcaggcgttc ctgaatatct gttgcctgtg
     6241 gcaatgtgtc cggggtaatt ggatcaggcg aaacgtcaaa actgacagga agagaaaatc
     6301 ccccggcgct gccttctggc aatacaaaag ttgctgtggt tgtcccctgg aatatttctc
     6361 gtatccgtgt gctgaagtcc tcgaccgtaa cgccgctcag ttccttatat gatgtgcgta
     6421 acagcccgtc attaccggat gtgctgcatt cagcaaggtc aaaacgccat ccggcgatcg
     6481 ataagggcag tgcatcccat ttatcaatgc atttatcaat aaatggtttt atttcaggta
     6541 aatgttgcca cggtggggca atctgtattg cctgtttgtc tgccatttct tttgccagac
     6601 gaatacgtgc agcttctgcc gcagcttctt tttcccggta ttcctggtac atggtgatcc
     6661 cgttccagag cagtatggcc agaatggcac ttccgccata aatcatgaac tgacgttttc
     6721 gggatacgcg agtaaacgcc gcctctttcg gcggtttttt tacgtcaatc aacgcagaca
     6781 gagtgagtga ggggagtgcc tggctgatat cccatgattc aggctggtat agtttccagc
     6841 cggggtctgg tgtttcatta aactcaagaa atgttgtcag agcactcatg atctgacttt
     6901 tgttacctac aacatcgttg acgagaacgt tattgacgca ggaaacaaag ccataaagat
     6961 caccgagttc gcagatagcg tatccgtcag gttcgatgag gggttttatc atcagtgcca
     7021 gtgaccagaa tgaggatgcc cctgtgggaa gatcctcttt tctgataaat gccaccatcg
     7081 ttttggtttc attttctgaa tcctgatact gacaggagag aaaatattca gcgtcgtttt
     7141 ttcttgcgaa tgtacgcgca ttcttttctt ctgaaggaag cgttttccag tccagaccgg
     7201 agaaaaaagc acgcttgttt ttaataaccg aagtgaaatt ccggttattc tgattattct
     7261 cagacaaagt gacctccaaa aaaactccct gtgttttttc agggagtttt cagttaatta
     7321 gttcacattt tttattactg cactggagcc ggaattaagc acatttggtg taatcaaaac
     7381 gaccagaatc gtgtcgccgt cttcgctgtt aacgccaccc ccaagtccaa agaaggaggg
     7441 ggttattaca ccttgcttac tggttttccg gtttgattgc tggaatccac tcagaatgac
     7501 tgtctggcct gatttcaggt caatagtctg attaattgtt ttcaaatcca cattcggtgt
     7561 ttgtgcttta ctgccgccac tttcgaacac ctcaaaagta ggtttgtcag acagactcat
     7621 tgagaacagc aattgcaggt tttgtgaatc cggctgaatg tatggaagca gagtcatatt
     7681 gaatccggtc gttattgttg ccgcattcag tgacgtggat gatcccacat tggcggtaga
     7741 atcggttgtg acactttcaa tgaagccctg ctgattggca atttgcatag ggacaggcgt
     7801 caggttttta gtcacagcgg agttttgcgt tacgacactg acgctgcctt gtgacgacag
     7861 ggctttcaga aatgcttttg agccagccag cttaccgtcc acgatggata caccactggt
     7921 aataacattc tcggcggcat caccaaatga gccaccaaga ctcagaccaa gatgaccatc
     7981 attaaatact gcgttccagt cgattcccgc ctgttcctgg cttgatttct tgatactcag
     8041 gatttcaacg ttcagcacaa cctgacgact catttcatta ttgcgcttgt tcacaatctc
     8101 ctgaacgctg tccagtacat ccggggtatc tgttactgtc agtgaacctg tggacagata
     8161 catgcgccct gtacctggtg tcagcattga ggaaacttcg cttttcaggt cgttgtacag
     8221 cgatgatttc atttctacgg ttgtagtttg cgtatttgat gcgtcgccag tcataccgcc
     8281 agaacttgta gagccggtac tgccactgga agacattgtg ccgctcacaa cttttgagtt
     8341 gtatgccaca ttgctgtcca tatatgtaat cgggaatgag cgcgtttcca gatagaagaa
     8401 tgtgatacga cctttatcgt atttccagga tattcccagc cgtgaagata cggtttgcag
     8461 tagggcagga agcccggaga tattcagttc agggagtgat gtaccgccag aagagacttt
     8521 tactggctga ctgcctactg acgccagagg aaccattcca tttgcatcag gtgctggaat
     8581 tgagcctgtc atttgttgtg ttgagccagt gcctgtaccg ttcagatagg tccagacatc
     8641 ggcgctaatt cgtaccggga tatgacaggt ttgtgtgatg cgttgtgcga tctggcgtag
     8701 ctcaatatct gaacgagtat taatcacaat cgggcagggc ggcgctgatt gctttacctg
     8761 cttttcaata attggcgttg tattaatcca ttgttttgtt gaatcctgga taaccgggtt
     8821 attacgaatt tttttcgcgt attcagtcgc tgtttgtgcg tctctttcgg cagcaagttc
     8881 attagcattg atttttttga agtttgcaca gcctgataat aaaaaagagg atattaataa
     8941 acaaagcaat tttttattca tgtttaatta ccttatggaa tatgcaaaat actatttgct
     9001 ggttgcgagg attattcaga tacgtctgtt ttatcgtgat tatcaaggtc tggattagta
     9061 atcacattgc ctccggcaac catgactttt tttcctccag ctttagggag ataaatttct
     9121 gcaatttttg ctatttcatc catgacttct tgtgtaaaca ggtttggaat gtcaacatga
     9181 ttgttacggg cgatatccac gcggcgttta aactctggtt cttcaaacca gattatttta
     9241 ttggcaataa atggtcggct aaattcactg gttataattt cacggattgc aatatttctg
     9301 gctttattct tcttatcccg gagagacaca atttcttcag gaagaagaac cgggcgctct
     9361 attacatcat cattccgtgt acgtgaactt cctcccttac caccaccact ggatattgag
     9421 cgtttggata ttttcatatc acgcacccca atctcttctg aaatcttttt agccagagca
     9481 ttttttgatt tcggcggata gtaaagcaca acagcactgt tttcagtgaa tgtggtccat
     9541 ccttcctgac cgtacatatc gcttttttgc ccttgtcctt cattctgaag aatgaacata
     9601 aagcgaaggt tataacctgc tgtaaaacca acagcgcgtt caatgacctc cgatttaccc
     9661 attgatgtaa attcatccag gagaataaga cactggtatt tcaggtccgg gttgtgttcc
     9721 ggtagttcac gacagttttc attaaccagt aatgagaaga aaaggtttac aattttctca
     9781 tgagtgatta aagcatccgg tgtcagacca agataaattg acatgggctt tttacgaata
     9841 tcccggatat caaaatcact gaaattcgtc gcctcagcag taatagggtt gctgaagatg
     9901 ctcagcggtg atgaaaagtt tgtttctata cttccccttg tgcgatctgg tgctgacatg
     9961 aacttaaaga agaatgattt tgttttatcg ctaatccagc tacggttttc aatttcctgt
    10021 cccatccatg cggcgagatc cttgccgcca tcgggaaccg acgttttcag tatggcgctg
    10081 atggagacaa gaacgtccgg tacattatga cctttggctt tctgttccag gtgaaaacgc
    10141 tccttgtcca gcaggtaaag tcctaacccg acaaaaaggt ttcgtgcaga atcagaccat
    10201 ataggatcat cacttgccgg gataaggatt gcggcaattt ttgccaggtc cgtttcacga
    10261 agtaaatcac tgcgactgac gcaatccaga ggattccagc ggtgagagcg tatctgtcct
    10321 ttaattgcct ggtcgatagt ttccgcgtat ccggcgggcg caaagagaaa gcattcttgc
    10381 cctaattctt tttgcctgaa tccggctgat aaaaaccagt tttcaagttt gatatccagt
    10441 atgaccatac ttcctggata gtttacgcag ttggggataa caatccccac ccctttacca
    10501 gaacgtgtcg gggcataaag aattaaaaat tgttgtccgg cgaaataaat aaattgtttt
    10561 ttgtatctcc ccttgaacat tttaccgatg agtattggtg gatgtttgta aggtgatttt
    10621 ttatccggga aaaaacctga tttactcagg tccatatcag ttgcgagccg ggcgtcaccg
    10681 tagatgactt ttttaggcat aatcccaatc acgacaatta acatgaatat ggcaaagaat
    10741 attaatggaa cgctgaatcc aaaccatgcc ataaaaacga gttttttata ttgtgggttg
    10801 ccagaaaatg ttgagattac agaatggaat gtatcccact tgagcaaaga catatcaacg
    10861 gatgaatatc ttaatgctgt gtatccaccg agatagtttg aggctataag cacacccaca
    10921 agaagcagta acaggaagag aattaacccc cccgtctttt ttgcatccat agttatctcc
    10981 agaatttaca ttgtgctcaa aaagcgattt ttttcttcgg gttcgtaata cagttcagtc
    11041 attctggtat ggttgaaaaa aagaatgaca tcaattgatg tttttatcgt gcgcatgatg
    11101 aggtccatat caacagtcat accaacatca gattgtttta caatacttgc cagtcgtgaa
    11161 aatgaggcat aagtattatt agcatgaatg gtactgattg agccttcatg ccctgtatta
    11221 agcgcctcca gataactcca tgcttcgtta cccctaagtt cagcaagaaa gatgtgatct
    11281 ggctttaatc gcatacacgc ttcaatgatt tccttcgctt caacggtatt ttttttgtag
    11341 aaaagccgga tgtggtttgg atgcagcggc agagacattt ccggtacatc ttcaacggtt
    11401 atataacgcc gttcaggtgg aaagatatct gcaattgcct ttgctatagt tgtcttaccg
    11461 gagccagtcc caccgacgat taaaaaatta agacggtctt taactgcctc tcttaaaaat
    11521 tgagctttag attgaccatc tggacgccgg gataattcat acaggtagcg ttgccgttca
    11581 gtcaggatgg cttcgtgttt cgtcgcaatc ctgacattat caaaacgccc tgttcggaca
    11641 taatcatcta tagtaaagcg cgttaatgat ggcttacgga tagatatcac aacgctgtta
    11701 ttttctgttg caggtggaat gatgatttgt ccacgttccc caccgggaag aactaccgat
    11761 gcaatgggat tatcatggct aagtggtatt tttatctttg ataagttcgt cagagttttg
    11821 gcaagtgtca ttaaattatc aaaagtcgcg tctggcgcat ctttagattc ccagccattt
    11881 ttcccttcaa accatattgt tcctggttta ttcacagata tttctgtgac atgttcaata
    11941 ttaagaacag actggatacc cgtcatatcc agatatcgtc ttgcggtttt actgttatct
    12001 cgtggttgtg atgtattact ggtatctctg atgttattca tatcaattgc tttactgaat
    12061 tactgaatta ctgaattact gaattactga agttcatata ctgatgaaaa gtcgatgttt
    12121 cgcggaacaa taacagacaa tacagtcccc tgattaatat atgccgtggg gggaatattt
    12181 atcgtattgt ccagcgttgt ttttgccatt tcttttgttg cgtcagatgt gttttcgtag
    12241 gtgatattgc tgtttgagcc tgtttgttgc gtgctgtttt tcagaatatc taaaccatca
    12301 ccaagtaatg acagcagcaa tgcaccacca aaacgttgac caaaatgatt atcgacccat
    12361 gcaggaaggc cggaagcgcc cagactgtca gtgccaagtg cgccaatgcg cacattgaca
    12421 ttttcatctt tgagcgttgt ccagttaacg aacacacggg ctactccctg tgtcatgact
    12481 ttattctgct ccccgatcag taatgctcct ttacgggcaa gaagcacttc gccgttatcc
    12541 gaccagacgt ctcgtgttaa ctgacacatt gtgataccgg gataagaggt tacgattttc
    12601 gttttgagca cacaggataa tgctgttccc gcagataaca gatatcggcg gttcagcaca
    12661 ggggatacct ttccgtctgc gtactgactg ccctgtaaat catctctttc ctggtttttt
    12721 tcgcctggtt cctgattgtc gatcttcacc attgtgtcgc ccatcagttg tcggacggat
    12781 tttggcaatg gttgtggttg gtttggatct ccactggcgg tttggggagt gccgttggct
    12841 atcacattga cggctgtttg atcggccttt tgtgatgctg gcgctgacgg ggcattgtct
    12901 tcctgtttct gttcttgctc ctcctgtgct ttcctgcggt tagcggcatc gagtttttcc
    12961 ttttcttcga tgtttttcat cattgttgtc agtgaaacgc cattgttggt atttgtgacc
    13021 agtgtctcgt cagacttctc aagaggtgtt tgctgaacga cctgttctcg tgaaagaact
    13081 ttaaacaaga taatgatcac aaataacgcg gcaatcagta atatgatgag gattaatatt
    13141 ttttttggtg ctgctttacc ttttacttcg ataattccgc gatttcccgc gtcattattt
    13201 ttttcatcat ctttattttt catagctgaa cttctctact cgtgtttttc cgtttgacgt
    13261 acctttttta ttccacggac gattaacgat tgatgatgtt ctgatatctg caacctgatc
    13321 acccaggcgc aaacggagat tttcagccac gtcatagtaa accattgtgt ttttatttct
    13381 gtctccgtta accagatgtt catttccatc ggcatcaaca cgataaagga ccggaatttc
    13441 ctgtttatta gtccatctca tgcatgtaaa ttcgccatta tcccatacct gataagggaa
    13501 aattgatttg tcccccttga gttgataatg accattaatt attccaccat cagtgcatgg
    13561 aaatggcttt tcagtcatta cggtagctga cggtggtttc ggataattaa aacgcagaat
    13621 atacgtgggt tgtttcgtgg ttgatatcag ataaagagga tatatatgct tattggtctg
    13681 aacggtaacg tttgtatccg gttcttctgc ttttggacgc agaaacagag tgttgtccct
    13741 gaccgatact gaccaggctt ctggatcacc aattccgaca tcttttattg tttcgtcttg
    13801 tccgaactta attgttgttt gtgcgcctgc ttttacgcgc acattaaata catcctgctc
    13861 gttataatct acggtctgaa tacgcccgtc tcgttccgat ggtgtgccgt acatagctgc
    13921 taaagcacca ggtacttgta gcatcgacaa aagaactatc gcagtagtgc gtttaagcat
    13981 cattttataa cctctggatc aggttggtag ctcagaactt gcagtcccag cggattcaca
    14041 aggcgctctt tttctgtttt gatatcttta tcccagtcaa aagaaatcgt cgaaatccac
    14101 tctgttgctc tgtatccggg ggcgggttta ccagataaat ccagtaccat ttttttgaaa
    14161 cgcacctgcg ccatatcctt ccttagaagg attactgaat taatctggac cttaattttg
    14221 tagttgtttt tcagaatgtt cagtggtgag gaatcagcac gaatcatggt gtcataagct
    14281 gaaaacacct tttgggaaga cattgttttt actgtatcgg cctgttcctg gatagtctgc
    14341 cagtcgtatg cttcatagtt aatgagatat tttgacagga aatatttagt ctcaacatcc
    14401 tgataacttt ccttagcatc agataattga ggggcaatat cagtatatcc agtgctgtta
    14461 tccactcgca caaggaacgg tacagcggtt tttaatggag caagggaggc tattgcgaaa
    14521 ccaagaatgc aaattacaac aaattcacca atggcgagaa tggtaaatgt ttttgccttt
    14581 ttctttatga tttcaatttc actgcgctca aagtcacgta tggccttgaa ataatccttt
    14641 ttttcgttct tttcttcaaa tttctttttg gaggcttctt tttttgaatg ttcataatag
    14701 tttgtgtttt ccattaataa tcacctgctt tgatctgttc aattgctgtg tttaactcaa
    14761 accatccccc ttttttctgc acaggattgg gggtgtgaga acatgcgcta attaatagcg
    14821 cggaggcaat tatgaggggg attttcatat tgttttccgt tctgtataaa acagagaaat
    14881 taaccgttca ggcgtttcag gagttctggt atccagatat caggagctac atctcctttt
    14941 tcagcaataa tctcatcaaa aatagacagg ccagtttcag agccagaaag cacggggata
    15001 aactcatcga atccaaacaa atccattttg gcgaaaacag aagagccaga ttgcttgatg
    15061 agcatggtcc ttgactcctt cgtcagctct ttgagttttt caaactcttt ttcagtaagc
    15121 ccaatttctt tatatccttc ccattttgca tcgggattgg gcaggagaat tttcgttgcg
    15181 gtttgttgta ccagcgccgc aaaaattgcg cagtttatgg cgtcttccgg ggactgtgat
    15241 gttaaccaca tcatttcacc tttcatacgc cccgctttca gtgcgctttt aatcatgctt
    15301 tgtgtcatgg gaaagtttgc tggcatccag aactcttcaa taatggagag catcagattg
    15361 ccgccgcgtt gcatgatttc tttgtagaaa aacaggaccg cgagtaaagg ttcacaggca
    15421 ggatgaatac cgttttttgt gtccaggaca acggttgtat caaatcccac ttttttatga
    15481 tgcagtggat tgaaggtatt ccggggggaa tccaccgccc aggcatattc gccgttgtcg
    15541 caccatttgg cgagacgcgt ctgaagttca ggactgacta tatcgagaag aagggctgtc
    15601 cggcgttctt cgacaggcat tcgcataatg ctttccacgg cagcgtttaa ttcaatgcca
    15661 tgttcatcag aacaggggtt gccctggttg tctcgtgcca gaacctgtgt ccagcgtttc
    15721 agaaatgcga gaagtctgtg ccagacggga gattccgggc ctgctgccag ttgccacgga
    15781 ttgcagcctg tataaatgcc ttcctgcaac gtgaaatagc ttcctccgta tgcacggaca
    15841 aaaagctcag tagaacggtt gaaatccaca acaaacatta aagggtcaaa gcgttgaaga
    15901 aaaccgctgg ctgctgcttc aaatgttgtt ttccctgtgc ctgttgcgcc aagaatcatt
    15961 ccatgcccgg caattttttg tccggtaaca tttttttccg gtgggctgta atgcgtattg
    16021 aaccagtaaa tgctgtcaga caccgttttc aacggcatta tggcggaccc atcaccaata
    16081 ggattgcctg attttttccc ggaggaataa ttgtgaaaag acatcagaca ggccaggttg
    16141 gttattgttc ttcgggtatc cagtggacgc ctcttattca gcggcatatg actgaaaaaa
    16201 acaaaaggtg atgcgagtga ggcgcgactg aagcggaatc ccttacccga agtaataaac
    16261 tcagcagaga gctttatacc attgcttctg gcctgatctg gtgtatcacc gaataccgtt
    16321 aatgcgcagt gtagtgaacc aaacaacgta attccggctg cgaccgcttc tttaccagct
    16381 tcgagttctt cctgttgtgt tttggctgca tcattagctg attgcagttt gtttaattgc
    16441 gaatcaatgt tcttcagggt ttttgtgggt gattcaaaaa taaacgattg agtaagaata
    16501 aattcatagg gttgcttgag aagaaaatcc cattgtccgg gggtcgtttc tatagggaaa
    16561 tccttcaaaa tataattcgt tgcaaacttt ttactatccg attcgttgtt acgcagttca
    16621 agaacatcag caccaaaata ccattcagaa tcacaaatac tggatgataa gggagtggat
    16681 gataaaggaa tcatgttatg ttctgtattt agcaatagtg ataaatagtc tgccacctca
    16741 ctaatataag tattgtgaac tgataaaaca gaagcgttaa atggctgaag tgctttttgc
    16801 gcctgttgtg tcatttcatt catgcgctcg attccggtat caacatcatc gtagggaatc
    16861 ccaaatgtta aataccatgt tgatttgtag aatgcgctgg atgtgaacag agcaaggtat
    16921 ttttttgaaa acctgcttaa aaaagcattg tcaaaattcc attctccatc aatggttatt
    16981 ctttttttga taagatgagt ccataaatac aggtcgcctt cctttcctaa cccaatcagg
    17041 aagtttttaa cgctgttaaa taaatttgta agaacattat cgttctctgt ctcaaatgga
    17101 atgcctgcta caacaagtga agccagcatt ttcctgtcag aagtaaagat aatactgtct
    17161 gttatatgga agcgataatc gggatattca cgagcaacat ccatattgtc actgtttttt
    17221 aatttgattt tgtgcatata tgttacctga cagatgaata accaataatt aaatctctgt
    17281 ggcggatttt taacaaaagc cccataaggt ttaaacggat gactcttagt gcgtttgagt
    17341 cattttcaca ggccagtttt actataaaaa gaaacagcgc acatatgaca ggaataatta
    17401 tcccttttat accccaaagg aaaatagcgg ggaagcctgt cagaacagcg aaaaaagccg
    17461 tgaacaataa caacattaag ggtataccag caattaacgc aggacggtta aacccgttat
    17521 aagtcatgaa tgactcactg acatgttcat ttactttgtc cataattcac catgaaaaaa
    17581 gccgcaattc acacataagg taaatatgcg gcacagttgt atatatggat tacttatttg
    17641 aagagttcca gcgcccagtt cccggcgtaa agtgcaccgc cagcgaggga acaatagaca
    17701 agcgccattc ctacatcaga ccagcttttc ttctccatga atgccatgat gacgttatag
    17761 atcaggtaac acaaagcaca aacaccacca aagctaaatg cccatgtctt aagttctgtc
    17821 atggtatttg ttgcggtgtc gagtccacct gcataggcaa cggaggcagt tagtgacagg
    17881 aaaaacgcca gaataaaata ctgcaaagtt gatttgactt tcattaacta aaatctccaa
    17941 aaacatccca gtccggtgat gttttctctt catcattcgg ggggtttatt tctgattcat
    18001 cagttttttc acggattgat ggcacgatat agttattcgc gttacgaata acccgttcca
    18061 gataagaagt gttgttaaat ttcttttctt taataaatcc tgttgattcg ttacccgaat
    18121 aatacaaaga taaagcgcgg agaatttttt gattactact accctccttt tgtctcagat
    18181 aattgtccgt caggactgcc gctcctgctt ttatatttgt acacgggtca aaaactgttt
    18241 tatttgtcag attgagtgat gggaaatttt tgctgtatat ctgtgtcaga cctgcactaa
    18301 accttttatt ttctgctgtt aatttttctg catattcaat agctcctttt tcatctttaa
    18361 aatacttact tgttccatca gaaacattcg ctattacata tggattagcc cctgattcag
    18421 tcataatcag ggcattcatc gtatcgggtg atacatcagg ggcacactga cttatcagtg
    18481 ctgccagaac agccgctgat aactgcataa gggatttatc tcctgatatt aaattcgtga
    18541 agaacgtttt ttcctgcggg ggcgacgaac gatactgtcc agctcattag cgccggaaag
    18601 aagttctttc agcgagacaa ttttttccct gacaggtata agggattttg cccccggaca
    18661 ttgttcaaag tctgatttgt acaggtcgta taaaacaagc gtatgtttta catcctgtga
    18721 cagataatta tgtttattct ggatgttacc ttgttcgata tttttgataa tatctgctga
    18781 acgatgaatt aaatctgtgt agattcgggt gtttttaaga ggatttattt ttatatattc
    18841 agaagcaagc tgaataagat tgcatcgtaa gataaggact tcacgagtgg caatatcaag
    18901 ctcgctgaca tcatcattat cctgcatggg tatctccggt aaagattttc tggactgaag
    18961 tccgcgccgt agcgatatat ccggcccaca taatgatgtg tcggatatgc agactccagt
    19021 tcagaatccc cttgtcaggg gatatttagt taatgagtac aatagcctct tctggaatgg
    19081 aggaaggaac gggaacgccc atatcattcc cctgattatc gacaatcttc ttatttttta
    19141 ctcttcctaa cattgctgaa tgttttgttt gctgctgcaa agcagacatc agaccttctg
    19201 tatcggcaga ccagatataa actcgcttac cgtaaataat atgatgaata tcataaacgg
    19261 gtgttatacc gatttgctca gatgttaacg ttcctcctga attacgtttg tctggatatt
    19321 tataaagata gtcattgata acatttgcga aatggacaaa ttcaattgct tcctgttttg
    19381 caggaagcaa cttcattcgc tgtatggttt ggttattgtt atctgtcaga tagtaactgc
    19441 ctattgacag gaatatcagg agcaagctga agaaccaacc catagcgatt actcctgttg
    19501 tgtgtcttta attgtgatta cgcattgtgc aatattcctg aatgcgtata acggtttatc
    19561 tgctgaaaga taaagcatca gcgtatcttt taatgcagat tcaaagtttc cccggaaaat
    19621 aagaggagca tctattctgt agttcacagg agtatcccat acaagattcc aggttgaatt
    19681 accacacggc gaacttgacg cccattctgt gaggccatcc cgcagcgtcg aacctttttt
    19741 aagattccag gtgagttttt ctgtctgttt ttgcggaacg gtgacaggcg ttagcgattt
    19801 ttgaggtaac ttgtcagagg aaacgggtgt cctgacagga gatgcagggg ttttgtcagg
    19861 agatacaggt gctttttgtg atgtttttcc tttctgttgt tgaaaatcaa tcagaagatg
    19921 tcttttttcg gtgttcttaa ttaccgtcag accgtagttg cgaagcattt tatccaggac
    19981 ataaggccat tgatcgttgc cctgccagga tatcgtgcga cggaaagttt tcgttacctc
    20041 cggggatatt tcagccgtcc agtccgatgg gacaatttct tttaatgctt tttgcagcga
    20101 gcctttacca tcatggcgaa caaagtaaat ttgcttttca ggtgttgatg ggcctgtaag
    20161 cgtggtggcc ggaacagcgt tgttagcgtt ttttttcaga aaaccgcttt ttgacattag
    20221 cggtggttgt tcaggacgcg gttttgtctg ctgatcagtc ttttgtgccg tttgtgtctg
    20281 atggttgttg ctactgattg gctttgcggg agagagtgct ggttgttcag aatgctgttt
    20341 agttgctcct ttggctgctg tttttatctc accagatgaa gttatgctgt ttgttgtcgg
    20401 cttcagaaaa ctattattgc tgccctggtt ttttggcgct gtcgctgatt gctcagaacg
    20461 tggttttacg gtttctgcaa tacgaggcgc gggagttacg tttatggctt cctttttgag
    20521 ataattatca ttgctgtatt gaacagtgct gtttggctgt gacacgggct gctgaaggat
    20581 caccccgtta tagtatgtgt ctgctcttac ctgctcaatt gtggtattca gttcatacca
    20641 acctcctttt gcctgtgcgg gttcaggggc atgggaacac gcattcagca ataatgtggt
    20701 gggaataata atgagctttt tcatggttaa ccccaggaac gtccgaactt actcatgatt
    20761 tttgagatcg ccgcagggtc tgcggtcgtg cattgattaa gaaacgcttt tcgggcatca
    20821 aaggttttgc tccagcgaat agatcccttt ttctttttta tgatattaaa gaagtccttt
    20881 tcggcgcttt tgcattcgct cccgccgccg ctaccgatag ctttaccgta cagacaaaga
    20941 acggaagcac acggatcaga cgcatatacg ggggcagaga gtgcgatagc acttgcggcg
    21001 accatcgata aaatgatgga tttcatggtg ttcctccata tttcagacaa aagaaaaccc
    21061 gcacgaggcg ggtaatttaa atatcagggg aatcaacgca cgtgtggcgt aatgatgaca
    21121 atccggttct cactttggct atcagcatta actccagtaa aaccactcac gataaggggc
    21181 tggtccggtt tgagtaaaat attctgctcg acagtctgtt tccgtatcgg gtcagtggtt
    21241 gtgagtagca gatggaaatt tccacgctca atctccggtg ctatccgcat tgtgaagcca
    21301 gcagtccgat ctttttcagc cagtgtagtg tcaccggata ttgcctgacg ggggatcagt
    21361 tccgtggcag gttcccagct tccggcagat gttgtttgtg attcaacgca cggggtactc
    21421 tgcattattt tgcggattgt ttcggaggat atcgcctgac cgtcatcaaa gaatgcacta
    21481 cggaaaatca cgttctgatt tttggcaaac aagcctggat gccgctcgct cagactttcg
    21541 tatatattca taccattcaa acaaacagga gaactgtccg gcaggtctgt tcgcacggta
    21601 tcaattttca acgttatctg gtggtcattt gaggttaacg gtgactcagg tactggttgt
    21661 gttttgctat ccgcgcagcc tgacagtaat gcggtggcgg caaccatcga taaaatgatg
    21721 gatttcatgg agttcctcca tatttcaggc aaaagaaaac ccgcacgagg cgggtttttt
    21781 atcatggcat aagtttattt attggagcgt ccaagcattt aactcatgat ttcttttcgt
    21841 tttagatcat agagtgcttc gttagcattt tatgtattcc ttaatcaagg cgtcttctca
    21901 tttcactaac tttcttttta tattcactat cttgtttttg ctgaagtgca gcacgagcat
    21961 tatcgcagtt ttgatcggtt attgtacctt tcttgcaatc ttctaaagtt ttagtggctt
    22021 cactgatgtt gtttgagtaa tattctacag aatatatttt ttctttacat cccactaaca
    22081 ttaaactggc gaagataatt gatgaggcga taattgcatt tcgtgactta ttcatatcag
    22141 cttaaatctc ttctcttttg gcgaacatat gtttcatatt gagctttccg ctcattctct
    22201 agttgccgtt cagcaatatc atattgcttc aactgtagct ccattgtcaa tttttctgta
    22261 ttcaattggt tagataagtc agcttttttt tgcggagtat ctgctgacgc gaactccttc
    22321 tgtaacgtgg ttaatctctt agccctttcc tgcatttcat tgttgaaatt ttcatagaat
    22381 ttgatcttag ccaggatcgc atcatatttt tgttgcgtct ctgcaacgtt gcttgataag
    22441 tcatattgcg atcgcaaaga gctaagatct gattttgcac tatttgttat tgttgataga
    22501 gaatccttca tatagctgga tgcgctatca aagccattgc tgaaatcagt gaagccttcg
    22561 agtcttttct tctgttcttc agcgtatctt atggtctgtt catattgttg cttgagttgc
    22621 tcaagctgtc ttgccgcttc agcagcacga tttagccctt cttcaactgt tttagctatt
    22681 gctgcaacgt ctactactgg aataccagat gcaaagattg atggtgatgt tatggtaatt
    22741 attccagcaa agagaaatga taatacttta tctttcatgt taatcacccc gctttaagtt
    22801 tacttccacg gtttaatccc tgaactaatg ctgatgcaga acgacttcct gcattaaaga
    22861 atggatttgg cattttgctg gcaaaaccat tagtgaaaga tcggagtcct gatgctcgca
    22921 taaaaccgga tgcaacctta cctccaaagc cattagcggc tgatgtaagg ccatttatac
    22981 ctaccccgcc agtaagagtt gagcagagag tacctacttg ctcaacaagg aatatagata
    23041 taacagtcac cataaagaaa taaactactg ttgtgagagt tatatttccg cttgatggga
    23101 ctgtttgctc tacaaagctg atgacgaagc tgaacgatat actataaaaa agatttagca
    23161 atatataatt taaacagcta cctacccatg cgctaaacat atttcttgtc gaagcaaata
    23221 gagaaaaaca gataaataat attccggcgg ataagagaat gccaaccatg aatgttgata
    23281 gagttagaaa tacagtggtg taataaataa gcagcagccc gccaacatat ccaacccccc
    23341 aaattagata tatgtatagt tgatctgtga agctatacca ctccagagta tctaactcat
    23401 tattcttaaa atcatttaac gttactgaaa gcatattcca caaattatca acactggttg
    23461 cagttcctga acctccagta acagcagcag aaaggtcgga gccagcgtgc attacaaaag
    23521 gaataacaaa ctggctgtaa taaggcgctg aataagtgaa tgcaccaacc agggcgaatg
    23581 caccgatatt cttagtcacc tcgctcatca ggacgtcacg ttgggcatac gtgatttcat
    23641 atattagata aataacgtat aaggctatag cagcaaagaa tgtaggagat attgcctgtg
    23701 cgaccttagc ggcgttcgtc gccgctgtgg attcaacaat ggttgttact ttctcaagaa
    23761 attctgaaac aatgttcata gcttagtcct tctggcagat gaagagttca ccagaggcac
    23821 taatatttgg cttcagctta attaccaggt ctgtgccgaa aaaaattgct tctccaattt
    23881 tttggaaatt taatgtggat tcttctttat tgtaactata aacgttgtgg gtcgctgtcg
    23941 atgttttggt tttgcgtggt gtaaacgctt ttttaatgat gtaatcgaga gcgtggctat
    24001 tatgagatgg taaagtgatt aaatcgtcgt tttttttcat aatgtagttc tgatttttga
    24061 ggttgaagat aattgtcggt caatgctttt gatttttttt cgtatctcaa ttggattcgc
    24121 ctcagataag ttagcaatgg ataaagccag ttcgatttgt aaaattttcc ttgtttcgat
    24181 gagattgatt tttacttgtg gtatatcacg gaagacacca cagccggtaa ttgcttttga
    24241 aacgacacgt tctgtattcg ctatgttttt ctttactgtt cgagggggga cataacgtgc
    24301 acggcgaagt tttctcagat tagcttttgc ttgagtcata tttgtatgcc ctgaataaat
    24361 aaatgctttc ttcaaagcct gaataagcat aaaccaggat tatggtggct aaaggcaaag
    24421 aaaaggtgaa atgaataacg gcattcagaa ctgaaccggt taatgtatat aagacaaacg
    24481 gtgctgaaag tatgaaagta cccagcagga aagaaaaaac attgcacgcc atttttaata
    24541 aaaatatttt tattaggtga tgtttttttc tgtacatttc atcgtatcct tcagggggct
    24601 gtcaccctct cacgttgttg ctttcatcag caagtgcctc aataagttta ttaatacacg
    24661 cccgttctgc cggaacctcc cggcctgtaa tcgggtcaaa aacgacatga tcgctttcaa
    24721 cgtaacgtaa acggtgatag acatctatac ggagttttgt tttagcagct tcacgttcaa
    24781 ctcgtgttaa ctcttctttt tctcgtttgc tgctgaaaaa cagaaaccag atcattgcta
    24841 tgataattac taaaaaaggg acaacggtgc ctaaaatgct taccatttct gccatgccac
    24901 ccagaataga ttgaatcttt cctgtggctg tggtggttgt tactggttca gcaagatcag
    24961 aaccagtgtc tgataaaaat gtaaataaac gggcagataa accaaatagc acgccaaaaa
    25021 cgagaacttt tattatcgaa ccgtatacat ttaatagact gtattcttcc tctttaggcg
    25081 attcaaagtt ttttgccctt tgtgtctgct cttgtttaga accagaaaca cctccgatga
    25141 ataagccaat accaccagta cctaaaccaa tacccccacc agtaccattc catttacctg
    25201 ttgttgtttc tttagctaca atagctgcaa gagaaatccc ttcatttgcg tttcgcatag
    25261 aattgtcctc atactattcc tgtgacactt aacaccatca ataaaatatg taatgcagta
    25321 atatttatta gcgttttttt gtagacttca ataaaatctt tatccagaag tctgataata
    25381 aaaatacata gtgcattcat cgctgttccc gtaagcggaa ttaacaacga aagaggcatg
    25441 ttttattcct tgctttcact cttgtcttcg atggtcatta ctttaagagt tattccttgc
    25501 atgtctgtaa caataactct tgttcccgca gggtagtttt catcgtgtaa cgtctcaaca
    25561 ggccagaatg tatcaccaat gagtaattgc cccttgccgt taacaatatc gtcactgagc
    25621 gtaaattctt ttcctttaaa acgtgaatgc cctgtgttca ccaggagagt atctaatttc
    25681 ttatgttttt tgtcatagaa gaacttaatt atcgacgcca gtatgctgat tgacgcaaag
    25741 aagcatatat tggcttcttg tgatatcgga agaaaaaagc ccacaatggc gcttgataat
    25801 gcaccaagac tgattaatag taaccatcct gttccggtta taatttcaag tgcaatacac
    25861 agaagaaaca gtgtaaacca taacatggct aatctccatg agataaggac ggtgattaaa
    25921 acaatattgt caccgtccgc tatctttttt aagacaaata ttgttgagcc tgtttcatat
    25981 caactttggg agtatcctgt gatacgtttt ttagcagttc actgatacct gcaaccgaac
    26041 tcaccaggct acttgaatca agcggcatca taaccagttt actgtttgat gctgtgccaa
    26101 ttgcctggag tgcttcagtg tatttctgcg caataaagta attaacagac tgaacatcac
    26161 cttcagcgat tgcatccgac accagttttg ttgcgcgagc ttccgcttct gcctgacgtt
    26221 cgcgtgcttc agactgaaga aatgctgatt gccgttcccc ttccgctttc agtatttgag
    26281 actgtttttc cccttctgcc ttcagaattt ctgactggcg tataccttcc gcttcaagaa
    26341 tccgggcacg cttagttcgt tccgctttca tttgcgcgtt catcgcttca gtcagttctt
    26401 ttggtggttt aacatcccga atttcaatac gtgtgacctt aattccccac gggtcagtcg
    26461 cataatcgac aaccgttaac agtttagaat tgatgctgtc acgttgagaa agcatatcgt
    26521 cgagattcat tccaccaacg acagttcgga tattggtcat aacaagattt gaaatagctg
    26581 atgcaagatt atcgacttca taagctgcct tcgctgcatc aatgacctgg acaaaacaaa
    26641 cagcatcgat agttacgttt gcattgtctt tcgaaataac ttcctgttta ggaatatcca
    26701 ggacggtttc catcatgttg atccgttgac ctatgcggtc cataaacggg atcaggaagt
    26761 gaagaccggg ggaaagcgta tgggtatatt taccaaagcg ttccactgtc caggcattac
    26821 cttgtggcac gattttgacg gcagatttaa cgaatatcag cgcaactaaa attaataaga
    26881 gtaacggaat agatgtaact attggcgtaa ttatagcgtc aatcatgatt aatcctctcc
    26941 caattcatca ctgtggatgt atttctttgc aaatgaaatc tgaaatatga acatcaacag
    27001 aaaaacactt tcctgcttct ttttcttttg agaagttttt tctgcattct tcagcggtca
    27061 tagggaagtc gctgtaatga gtccatttca cggggccacc acctggtttt ttgatggttg
    27121 cgtttatttg gtacttaggc attcaaaaat acctccactc gatcacctgt ctttcttgaa
    27181 cacatccagc ggcgcagttc tgtaaaacta tcctggatct cacaacgggt acttttcatt
    27241 gtgacagttg gtatattgtc gtagctggca tatgcaatct ctttttttgg ggtgcgcaat
    27301 atgcctttgt caatatcaag aatcctgaac gcgctaattc ttcttttgtg ctcgttaaat
    27361 agtgttcgtt tagttgctgt tatctgcttc tgtgcttcct ggtaaatgta ggtaagcaca
    27421 tcataagatt gttgctgtgt aataaggaaa ttacggctaa catccgttgt tgtgagcgtt
    27481 ttattatttc ttaatcccca ataagccacg atgatataga gtggatatcc tttcaaatct
    27541 gccacacatt caggaatgac aggattaatt tttaacttac gcgccatatt ttctcccggt
    27601 tgtttgtctg aaatagaaac atccccggaa tacagcgtca taagctaatt atgaaaagtt
    27661 gcagccactc actacaggct ctttatctga ttgttcatca tcatgcatag ctaaaccccc
    27721 ttacctggag tggagactgg caaatctggt gtacttctgc gttgcagcct gtacgacttg
    27781 ctcagggaga gagtgagtga gattaactca acgcgagttc accaaataag ggggtttaag
    27841 tatgcagaat gatatttgcg gataaagagt cgttacctgt gtagatggcg cggcacgtca
    27901 ctctgttacg tagcccgttg ctgacagatg ccagcggtgc ttgaaatgca cgaataataa
    27961 cctccttatg ccagggcttt aggcaatgac cgctgtccgg ggttgttaaa gagcatgaga
    28021 tgacaagcgt tctaacgggc gtcaaagagt gctcattttc caggcaatga gcacaaaaaa
    28081 cgcgatgaac gagcttttta caatcatctg gatagtattg tgtgtgcgac atttaaaacc
    28141 tgtcaatgcc acaagagcga tctaatgatc tggataacac ctaaaaaaca gaataatatt
    28201 ccgtttgtta aatgatgtac gatctaaaaa tctagataat actgtttgtg tggtttattc
    28261 gatcgtgtga gtgggtgtat gcagtcaaaa aaactttttt gaatatacaa aattctgaat
    28321 ttctgatttt gtaaggtttt gtatggggaa cgtcagtaaa gaaggggctt cagtagctgt
    28381 tctgaaaacg attttgaagc tgtcagagat gtgtgctgaa tcaaaaagga aacgagccag
    28441 gcttgaacat cgttgatcga ttgcgcccat gctgctaaat ttgcaggcaa gttttccgcg
    28501 atttgtaaat gcagaaaacg aggtgctgaa attttgttca agaacgtaaa tttcagataa
    28561 agaaaacccc cgtaatctcg tgttttccag gcagcgaaga ttagcggggg ctgacatcaa
    28621 ggcagagcct tgagcttatt tatttcaggt atgcttgtta cctgaagtgg agtttaattc
    28681 catgagaagg aaatatcaac tcttacagtg caagctaagt gcagcttgct gtgattagca
    28741 aacgtcactg gtctgaacta tcaccggaag agcaggtccg tttctggcag gactacgaag
    28801 cgggaattga aagctcattc ctggtccccc aggaaaacaa aggcggaaca acaaaacgtc
    28861 gccggggtga acattccact aagccaaagt gtgagaatcc agcctggttc cgtcccgaca
    28921 actataaggc gctgggcgga cagttgggcc acgcttacaa tcgtctcgtt aaaaaagacc
    28981 cggtcactgg tcagaacaca ctcagaatgc acatgtcgtt gcatccattt tatgtactga
    29041 aacgtcagag tgtcggtcgc aaatataaat tccgaccgga gaaacagcgg ctgcttgacg
    29101 caatatgggt tgtacttgtg agtttttgtg atcgtggatt gcacactgtc ggcatgtctg
    29161 tctcccgcct ggcagaagaa ataagcccaa aagacagtaa gggagacgtg attcctgaaa
    29221 ccgcagtcac agtatcgcgc ctttcccgtc tgctggcaga gcaggtttcc tttggcacgt
    29281 tgggaacgtc agaaaagacg atatgggatc gtgaatcccg ccagcgactg ccgaaatacg
    29341 tctggatcac cgaaactggc tggaaaatgc tgggagttga tctggtgaaa cttcaggagc
    29401 agcaacgtaa gcgccttgca gaaagtgaaa tccgtctgca attgattaaa gaaggcgtta
    29461 tccgcgaggg cgaggaaatc tctgttcact cggctcgtaa acgctggtat gcgcagcgtt
    29521 ctctcgatgc catcaagtcc cgacgtaaga aagcagcgga gcgcaaacgt gctaatcgcc
    29581 tggcaaaact gccgtatgat gaacagcgaa acgaaattgc gcggtttatt ctgaagcgta
    29641 tgccgccgga cgaagcgtac tggtgcacta aagaaagatt agagcaactg gtagccagag
    29701 atctacgtca gcttgaactg gctctgacag cttcgccacc ccactagttt agctgcctga
    29761 tacaatcggc actctcttca gccccttcgg ggcttttcgt gttgcctgca tttcatgaaa
    29821 ttcctcatat ttgacgaccg tttttcattt tgtcccgctc tgacagtttt ttgatggccg
    29881 caatagggta tcggagtaaa aacgccttcg gttatccaca aaaacccgca aaggaataac
    29941 ttcagaaagc agcaaacata gaacatcaaa cccgcaatat attcttaacc cctgttcttt
    30001 aatcccctgc gttgcttcgc cgcagggaaa atatttatct ttgaaacaac tgtggataat
    30061 tacaaaaatg ccttcgcttg cagcggctaa cgccgcgccg ctcagaatat taaaagtacc
    30121 tcccaccgct tcgcggcggg catatggact tctgcctaat gaaaaacaaa gcccctccaa
    30181 ccctcctaca aaaaaacgct gcaaatactc agccacagcg cccaacccat cactgaaaag
    30241 cgccgccgcc cccgcccgaa gggcgggaac aacatcgctt tcaataatgg atgttgtaac
    30301 taagaaatct catcgctgtc agccttctgg ctggaagtac caaatacacg ctcgtaagcg
    30361 gccctgacgg tccgctaacg cggagatacg ccccgactgc gggtaaaccc ttgtcgggac
    30421 cactccgacc gcgcacaaaa gctatttcat ggctgaaagc gggtatggct tagcaggatg
    30481 gggatgggta aggtgaaacc tatcaatcag taccggctta cgccgggctt cggcggttta
    30541 gctctgtgcc atataaaaca acagagtgcc gccttacatg ccgctggcgc ggcatcaaaa
    30601 agaaagcccc ccaataacgg ggggaaacag cgttttgatc aaaatgataa tgctagtgtg
    30661 cttcttgcgt gccattccag gcacaaacta aatttaacgt ttcgttaact acaaagaaat
    30721 ccgatgagtt aatcacttgc gaaataaaaa tcatatagta aattactata tgattttgcg
    30781 ttgtttcgga gaattaaaaa atgcctttga ttgaatacat caacaagttc tatcggggga
    30841 atcaggcatc gttcgccagg ctgaccggag ttcaacctgc gcaggtaaca cagtggatca
    30901 acaaaggatt catcgtggtg aatcacacac tgtatagtcc acgccgaaag ctgggaattt
    30961 aattcctggt caatatataa tgctgttggc gcttgaggct ttctgcctca tggcgaaaag
    31021 gtggtttgta tcttgttgtg cggcagaaag aagaaagccc cgtagttaat ttttcattaa
    31081 cccacgaggc gatcccaatg cttcaacaac atcaggatag cctcttaccg cgcgttacgc
    31141 aaggagaaga aggccatgaa acagccgaaa aacgccctga cctggtgttt attaatcgtg
    31201 tgctgcacat tgttaatatt cacgtatctg acccggaatc gtttgtgcga ggtccggctg
    31261 aaagatggaa acagggaggt tacggcaagt ctggcttacg aatccaacgg taagtagcaa
    31321 cccggaggcg ggcgaaagcc cgcctttttg gggatgatgt ggttctggca ttaagcgcct
    31381 ttgcaaaggg ggtattctgt acggataccc cctttttcca tgtattttca ggatattaac
    31441 tgctgaaaat ggtataatta gtcaatatga cagcaattat tgaggcgagt atgcagccaa
    31501 aaaaaacacc cgtgattgtg gtcaaaaaac gccgcgttct ggtcatgcca gaaaatcctg
    31561 tggtgaatga aaagccgcag gaagtacaaa aatcagcagt taatgaaaac aagaaagtac
    31621 agaagaaaga tgctgtagca gagaaaacac ataaaaagca gcctcagccc tggtatttga
    31681 aaaaacaaat cacttttccc ccaaaatatc cgaaagaata ttttgaaaag tgtttcaata
    31741 aagttcgtgc tgtttttcct gaactatgga cagacgaaaa aaagaacttg cccctgaaaa
    31801 gcgggattct ccaggacgtt gagaaatacc tggcggataa cccggatgtg gatctgacga
    31861 ttgaagagtg gaattgtgcg gttcaggtga tgacgttccg gtggcaatat cttcagaatt
    31921 gtactgtacc aggcgcaaca cgttacgacc tttacgggaa acctgctggt acagtaaaaa
    31981 aagcacacgc aacttatgcg caactggttc tcgatgcccg taagaaagcc agcgagaaga
    32041 aacaattaaa acgtaaggga taaagccccg aagggcttta tcatttcagc ctggctgact
    32101 gtagaatcca gtgatttttt ccgttttcgt ccaggccgtg ttcgtaagct gcaagagccg
    32161 ctttcattat tgctgttttt gtctggcctg ttcgcagtgc ttcccgttcc agaatatctt
    32221 cgtaaacgtc cccaagccgg acattggtca ctttagttgc tcccgatttt ttgcgggtag
    32281 cttcattgat gaatcgggct gtgtctgcat tggtttgttc tgttttttgt cctggtatta
    32341 ccggagtttt caagtcaaga gccattatat cacctttcgt gtgttacatt agctttactg
    32401 tgtaaagtta atgatgtaaa gctatcgtat tacatccatt agggattgat gcctaactct
    32461 tgcgccaaaa gctctatttg tgctttagct gtagaaaggc tggatgcgcg acgtacatca
    32521 tgaacgccag cgccttcatt acaggccgct tcaaaaatat ctaaatctga aatacgtgta
    32581 cgaagtggct gtatccacac cgggttctcc cggagtaact tgtcgagatc aatcgctgct
    32641 tttctgtggc gtggttttgt cgtgttaatt ctcgtgaaaa gtacccaggg ttgcaggtct
    32701 ggattgatct gttgagctgt gcgaactttt tctgttacat gagtcagcgt ttctgcttcg
    32761 aaatctgaag acggcttaac gagagtaagc agaatgtcag ctacagttaa ggcactgcga
    32821 aattccttgc tgtcatgccc tggacagtca atgatcagca cttcacatat ttttttcagg
    32881 cgcttaatga cttctgatac atcgccatac tcttcataaa caggaaccgg taataagcca
    32941 ttctgctttc ttttttcgtt ccagctaaga atatcattgt ttttatcggt cttcagaatg
    33001 atgaccgttc gtgaattatt taccagataa gaagccaggt ttgttgctgt ggtgcttttt
    33061 cctacaccgc ctttatctga tgcgacaagt aatgctctcc ccatagaaac ctcggatata
    33121 cagtgtaaag gtttatttgt ctttacattg tattcattat agttacgttg taaagatgtt
    33181 tgtgtcttta caatgtgatt gttttttgtg ctttacattg tgtgtatgtt acagggtaac
    33241 ttttcaggat gcgattttgc ctggtgtgtt gttgcgcccg tagctcgctg acgctcacac
    33301 gcgcagtaca gcgccccgcc ctgctgcaat gcaccaggac ggcgtaaact cgctggcgct
    33361 cgcgctaact gcggcattct gcggcgcaaa tcagcatgat catggcctgt tggtctgttc
    33421 tggctttgca gggcgaaggg atggccctgt gcatcctggt atgttcgtca gcggctgcgc
    33481 cgcgctt
//
LOCUS       DAZPDU010000029        32839 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZPDU010000029 DAZPDU010000000
VERSION     DAZPDU010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32839
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.029"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            267..1925
                     /locus_tag="ACHURS_001263"
     CDS             267..1925
                     /locus_tag="ACHURS_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFR1237043.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNMEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIVTTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1922..2878)
                     /locus_tag="ACHURS_001264"
     CDS             complement(1922..2878)
                     /locus_tag="ACHURS_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFR1237044.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3029..4144
                     /gene="macA"
                     /locus_tag="ACHURS_001265"
     CDS             3029..4144
                     /gene="macA"
                     /locus_tag="ACHURS_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFR1237045.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4141..6087
                     /gene="macB"
                     /locus_tag="ACHURS_001266"
     CDS             4141..6087
                     /gene="macB"
                     /locus_tag="ACHURS_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFR1237046.1"
                     /translation="MTPLLELKDIRRSYPAGNEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6160..6384)
                     /gene="cspD"
                     /locus_tag="ACHURS_001267"
     CDS             complement(6160..6384)
                     /gene="cspD"
                     /locus_tag="ACHURS_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFR1237047.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6534..6608
                     /gene="yljB"
                     /locus_tag="ACHURS_001268"
     CDS             6534..6608
                     /gene="yljB"
                     /locus_tag="ACHURS_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFR1237048.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            6707..7027
                     /gene="clpS"
                     /locus_tag="ACHURS_001269"
     CDS             6707..7027
                     /gene="clpS"
                     /locus_tag="ACHURS_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFR1237049.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7058..9334
                     /gene="clpA"
                     /locus_tag="ACHURS_001270"
     CDS             7058..9334
                     /gene="clpA"
                     /locus_tag="ACHURS_001270"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFR1237050.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9740..9827)
                     /locus_tag="ACHURS_001271"
     tRNA            complement(9740..9827)
                     /locus_tag="ACHURS_001271"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9791..9793),aa:Ser,seq:gga)
     gene            complement(10081..10299)
                     /gene="infA"
                     /locus_tag="ACHURS_001272"
     CDS             complement(10081..10299)
                     /gene="infA"
                     /locus_tag="ACHURS_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFR1237051.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10584..11288)
                     /gene="aat"
                     /locus_tag="ACHURS_001273"
     CDS             complement(10584..11288)
                     /gene="aat"
                     /locus_tag="ACHURS_001273"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFR1237052.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGAPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWHEDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFISHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQK"
     gene            complement(11330..13051)
                     /gene="cydC"
                     /locus_tag="ACHURS_001274"
     CDS             complement(11330..13051)
                     /gene="cydC"
                     /locus_tag="ACHURS_001274"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFR1237053.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTVGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13052..14818)
                     /gene="cydD"
                     /locus_tag="ACHURS_001275"
     CDS             complement(13052..14818)
                     /gene="cydD"
                     /locus_tag="ACHURS_001275"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFR1237054.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWAVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(14941..15906)
                     /gene="trxB"
                     /locus_tag="ACHURS_001276"
     CDS             complement(14941..15906)
                     /gene="trxB"
                     /locus_tag="ACHURS_001276"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFR1237055.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16451..16945
                     /gene="lrp"
                     /locus_tag="ACHURS_001277"
     CDS             16451..16945
                     /gene="lrp"
                     /locus_tag="ACHURS_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFR1237056.1"
                     /translation="MVDSKKRLGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17080..21147
                     /gene="ftsK"
                     /locus_tag="ACHURS_001278"
     CDS             17080..21147
                     /gene="ftsK"
                     /locus_tag="ACHURS_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFR1237057.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTAAQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYAPAPEQPVAGNAWQAE
                     EQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYY
                     FEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVETAAAVSPLAS
                     GVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYG
                     IKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQH
                     DVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGP
                     HEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPV
                     APQQQYQQPQQPVAPQPQYQQPQQPVAPQQQYQQPQQPVAPQPQDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            21302..21913
                     /gene="lolA"
                     /locus_tag="ACHURS_001279"
     CDS             21302..21913
                     /gene="lolA"
                     /locus_tag="ACHURS_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFR1237058.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            21924..23267
                     /gene="rarA"
                     /locus_tag="ACHURS_001280"
                     /gene_synonym="mgsA"
     CDS             21924..23267
                     /gene="rarA"
                     /locus_tag="ACHURS_001280"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFR1237059.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLGTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23358..24650
                     /gene="serS"
                     /locus_tag="ACHURS_001281"
     CDS             23358..24650
                     /gene="serS"
                     /locus_tag="ACHURS_001281"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFR1237060.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24889..27333
                     /gene="dmsA"
                     /locus_tag="ACHURS_001282"
     CDS             24889..27333
                     /gene="dmsA"
                     /locus_tag="ACHURS_001282"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFR1237061.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLVNQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            27344..27961
                     /gene="dmsB"
                     /locus_tag="ACHURS_001283"
     CDS             27344..27961
                     /gene="dmsB"
                     /locus_tag="ACHURS_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFR1237062.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            27963..28826
                     /gene="dmsC"
                     /locus_tag="ACHURS_001284"
     CDS             27963..28826
                     /gene="dmsC"
                     /locus_tag="ACHURS_001284"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFR1237063.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDSWAMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(28862..29488)
                     /gene="ycaC"
                     /locus_tag="ACHURS_001285"
     CDS             complement(28862..29488)
                     /gene="ycaC"
                     /locus_tag="ACHURS_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFR1237064.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29803..30951
                     /locus_tag="ACHURS_001286"
     CDS             29803..30951
                     /locus_tag="ACHURS_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1237065.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            31161..32591
                     /locus_tag="ACHURS_001287"
     CDS             31161..32591
                     /locus_tag="ACHURS_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFR1237066.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(32592..>32839)
                     /locus_tag="ACHURS_001288"
     CDS             complement(32592..>32839)
                     /locus_tag="ACHURS_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1237067.1"
                     /translation="DVDAELDAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWITERP
                     GFQIYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ"
BASE COUNT         8058 a         8031 c         8813 g         7937 t
ORIGIN      
        1 ggttgcccca tatagtcatt tgtctgattg acagtgtagt gcacgcaaaa gatttaatcc
       61 tttaggcgta ataaaaaata atatatcatg ctaattattt gattttgttg tttttgcaga
      121 cttatcagca agagggagta taacgcgatt attcgctcat ttttcagaca tttgccatgc
      181 ttaaatgtga tgtcatcacg tattagcaag gcctttcccg ttatactgcc agcgtaaagg
      241 ataagtcaca tatttctgga ggggatatga ttcttgagcg cgttgaaatt gtgggttttc
      301 gcggtatcaa ccgtttgtcg ttgatgctgg aacaaaacaa cgtcctgatt ggggagaacg
      361 cgtggggtaa atccagcttg ctggacgcct taaccctgct gctatcgcca gaatcagatc
      421 tctaccattt tgagcgcgac gatttctggt tcccgccggg agatatcaac gggcgagaac
      481 atcatctgca tattattttg accttccgcg aatcgctgcc aggccgacat cgggttcgcc
      541 gttatcggcc gctggaagcg tgctggacgc catgcaccga tggctatcac cgtatttttt
      601 atcgtctgga aggggagagt gcggaagacg gcagcgtgat gacactgcgc agttttctcg
      661 ataaagacgg acatccgatt gatgtcgagg atattaacga tcaggcacgc catctggtgc
      721 gtttaatgcc ggtgctgcgc ttgcgtgatg cccgttttat gcgccgtatt cgtaacggca
      781 cggtgccaaa tgtccctaat atggaagtca ccgcgcgcca gctcgatttc ctcgcccgtg
      841 agttatcctc acatccgcaa aatctctctg atgggcagat tcgtcaggga ctttccgcaa
      901 tggtacagct gcttgagcat tatttctctg agcagggggc cggacaggcg cgatatcgtt
      961 taatgcggcg gcgagccagc aatgagcaac gaagctggcg ctatctggat atcatcaacc
     1021 ggatgattga ccgacctggt gggcgctcgt atcgggttat tttgctcggc ctgtttgcta
     1081 ctttgttgca ggcaaaaggc acattgcgac tggataaaga cgcccgtcca ttgttgctga
     1141 tcgaagatcc agaaacccgt ttacacccca ttatgctttc agttgcctgg catctgttga
     1201 atcttctgcc attgcagcgc attgtcacca ccaactcggg tgagttgctt tcgttaacgc
     1261 cggtagagca tgtttgccga ctggtacgtg agtcctcgcg cgttgccgcc tggcgtctgg
     1321 ggccgagtgg cttgagtacc gaagatagcc gacgcatatc ctttcacatt cgttttaacc
     1381 gtccgtcatc gctgtttgca cgctgctggt tgctggtgga aggggaaacg gaaacctggg
     1441 ttatcaatga actggcgcgt cagtgcggac atcattttga tgccgaaggg atcaaggtca
     1501 ttgagtttgc ccagtccggg ctaaagccac tggttaaatt tgcccgccga atggggattg
     1561 aatggcatgt actggtcgat ggcgatgaag cagggaagaa atatgccgct acggtacgca
     1621 gcctgttgaa taacgatcgg gaagccgaac gagaacattt aacggcgtta ccggcgctgg
     1681 atatggaaca ttttatgtat cgccagggat tttccgatgt gttccaccgc gtggcgcaaa
     1741 tcccggaaaa tgtaccgatg aatctacgca aaattatctc gaaagcgatc catcgctctt
     1801 ccaaacccga tcttgccatt gaagtggcaa tggaggcagg acgtcgtggt gtggactccg
     1861 taccgacgct gctgaaaaaa atgttctcac gcgtgctgtg gctggcgcgc ggtcgcgcgg
     1921 attaaccgcg aaacatcgtg gccatttgtg gctgaatagc gtcgagcatc tcatagcgcc
     1981 gacggtattc agcccgtttt ttactggcga tttcggcaat ctcttttcgt gctatctgtg
     2041 ctggaaggcg gtaatggcgt tcagcatcac atacgccgcc aaccgattcc cagaaagcgt
     2101 tgtaatcagc gtggatcttg ccttctttat cgcgataacg caggctgcgg taaatatgcg
     2161 tttcattgct gacggcaata atctgctcta cctgcaaacg ttgggcaaac agacaggccg
     2221 cttccatcac gaggcgtttg ggaaatagcc cgtggcaggc tttcgtcgca ttctggattt
     2281 cctgatgtgg aatttcccat tttgcgcctt gcagtccgcc aataaacatc gttcttttcc
     2341 cctgatattc acacagggta aacgtgatct ctgccagagg aataccttcg ctgttgcgaa
     2401 acaggattgt gctgtcacct tctttatcca ttgagatcat catggtcagc tcaagcgtga
     2461 actgctcgcc gtttttgcct tccagcttcg ccagttgcag cccgggggta ttcaaatata
     2521 agctgaattc ttccgccgac atacatccgc ggagtaacgc ataatggtaa cgtaacgcct
     2581 ccagcaattg cttacggcta agattcgccg caaggtaagg gcgatgcaga cgcacaggca
     2641 gtcgcggttg gcgcgttaac aacacattga gattaggcca gtgggaaagt tcgttcatcc
     2701 actcaacgct taaacgcggc ataatcaacg agcgcagcaa aaatttctgg cgaaaactac
     2761 ggcgatgcca gaatttaccc ggccgacact gtccacgtgc cagactaaga aaaagtgaca
     2821 ggctgctgag agattcagat ggcgtaaagg tccgttcagt tagctgcgac atattcatga
     2881 aatcaatggt tatacatgac gtcgatttca ccattgcgta tcttaaccaa acatcaatag
     2941 tgtgattact aacgtaaatt ttatggtttt gttgatattt cgttgaagtt aatgacccgg
     3001 attggcatat ggagtattca gaatatttat gaaaaagcgg aaaaccgtga agaagcgtta
     3061 cgttattgcg ctggtgatag tcatcgccgg actgattacg ttatggagaa ttcttaacgc
     3121 acccgtgccg acttatcaga cactgattgt gcgccccggt gatttacagc aaagcgtgct
     3181 ggcgaccgga aagctggacg cgctgcgtaa ggttgacgtg ggcgcgcagg tcagcggtca
     3241 gttgaaaacg ctgtcggtgg cgattggcga taaagtaaaa aaagaccagc ttttaggggt
     3301 cattgatcct gaacaggctg aaaaccagat caaggaggtc gaagcaacgc tgatggagct
     3361 acgcgcgcag cggcagcagg cggaagcgga gctgaaactg gcgcgggtga cgtattcccg
     3421 tcagcaacgt ctggcacaaa cgcaggcggt ttcacagcag gatctcgaca ccgccgcgac
     3481 ggagatggct gtgaaacagg cgcaaattgg caccattgac gcgcaaatca agcgcaatca
     3541 ggcttctctc gatacggcta aaaccaatct cgattacact cgcatcgttg ccccgatggc
     3601 cggggaagtc acgcaaatca ccactctgca aggccagacg gtgattgccg cacaacaagc
     3661 accgaacatt ctgacgctgg cagatatgag caccatgctg gtaaaagcgc aggtttctga
     3721 agcggatgta atccacctga agccggggca aaaagcctgg tttacggtac ttggcgatcc
     3781 actgacgcgc tacgaggggc aaatcaagga tgtactaccg acgccggaaa aggttaacga
     3841 cgctattttc tattacgccc gttttgaagt ccccaacccc aatggtttgc tgcggctgga
     3901 tatgactgcg caagtgcata ttcagctcac cgatgtgaaa aatgtgctga cgatccctct
     3961 gtcggcgtta ggcgatccgg ttggcgataa tcgttataaa gtcaaattgt tgcgtaatgg
     4021 cgaaacacgc gagcgtgaag tgacgattgg cgcacgtaac gataccgatg ttgagattgt
     4081 caaagggctg gaagcgggcg atgaagtggt gattggtgag gccaaaccag gagctgcaca
     4141 atgacgcctt tgctcgaatt aaaggatatt cgtcgcagct atcctgccgg taatgagcag
     4201 gttgaggtgc tgaagggcat cagcctcgat atttatgcgg gtgagatggt cgcgattgtt
     4261 ggcgcttcgg gttccggtaa atcgaccctg atgaatattc tcggctgcct ggataaggcc
     4321 accagcggca cctatcgcgt agccggtcag gatgttgcca cgctggacgc cgatgcgctg
     4381 gcgcaactgc gccgcgagca tttcggcttt attttccagc gttaccattt gctttcgcat
     4441 ttaaccgccg agcagaacgt tgaagtaccc gccgtctatg ctggtcttga gcggaaacag
     4501 cgactgcttc gtgcccagga gttgctgcaa cggctggggc tggaagaccg tacagagtat
     4561 tatccggcac agctttcggg tggtcagcaa cagcgcgtca gcatcgcgcg ggcattgatg
     4621 aacggcggtc aggtaattct tgccgatgaa ccaaccggcg cactggacag ccattctggc
     4681 gaagaggtga tggcgatcct gcatcagctg cgcgatcgcg ggcatacggt gattatcgtc
     4741 acccacgatc cgcaggtcgc tgctcaggcc gagcgggtga tcgaaattcg cgacggcgaa
     4801 attgtgcgca atcctcccgc cattgaaaaa gtgaatgttg ctggcgggac ggagcctgtt
     4861 gtcaatacag tgtctggctg gcggcagttt gtcagcggtt ttaacgaggc gctgacgatg
     4921 gcatggcggg cgctggcagc gaataaaatg cgtactttac tgaccatgct ggggattatt
     4981 atcggtattg cgtcggtggt ttccattgtc gtggtgggtg acgccgccaa acaaatggtg
     5041 ctggcggata ttcgttctat tggtacgaat actattgatg tctatcccgg gaaagatttt
     5101 ggcgatgacg atccgcaata tcagcaggcg ctgaagtacg acgacttaat cgccatccaa
     5161 aaacaaccgt gggtcgcctc agccacacct gccgtctcgc aaaacctgcg cctgcgttat
     5221 aacaatgttg atgttgctgc cagtgccaat ggcgtgagcg gcgattattt taatgtctat
     5281 ggcatgacct tcagtgaagg aaacaccttt aatcaggagc agctgaacgg tcgtgcgcag
     5341 gtcgtggttc tcgacagtaa tactcgccgc cagcttttcc cccataaagc agatgtggtt
     5401 ggcgaggtga ttctggtcgg caatatgccc gccagagtca ttggtgtggc ggaagaaaaa
     5461 cagtcgatgt ttggtagcag taaagtgctg cgtgtctggc taccttacag cacgatgtcc
     5521 gggcgagtta tgggccagtc gtggcttaac tccattactg tcagggtgaa agaaggattt
     5581 gacagcgccg aggcggaaca gcaactcacg cgtttacttt cactgcgcca cggtaagaag
     5641 gatttcttta cctggaacat ggacggcgtc ttgaaaactg ttgaaaagac cacacgtact
     5701 ttacaactgt ttctgacgct ggtggcggtg atttcgctgg tggtgggtgg tattggtgta
     5761 atgaatatta tgctggtgtc agtgaccgag cggacgcggg aaattggcat tcgcatggct
     5821 gtaggtgcgc gagcaagcga tgttttgcaa cagttcctga tcgaagccgt actggtttgc
     5881 ctggtcggtg gcgcgttggg aataactctg tcactgttaa ttgctttcac cttgcagctt
     5941 ttcttacccg gctgggagat tggtttttca ccgttggcac tgctgctggc gtttctctgc
     6001 tcgacggcca ccgggatttt atttggatgg ttacccgcac gaaatgcggc acgactggat
     6061 ccagtagatg ctctggcacg agagtaattt ttgagataaa aatgccagcc gatcgggctg
     6121 gcattttgcc tttaggatgt acacaatgag acagaagagc tatgcgactg ccgcttctac
     6181 ttcgacgggc acaataacac tggcgtgatt gccttttggc ccctggtgga catcaaactg
     6241 aacggattgt ccagctttta gcgttctata accatccatc tgaatggtgg aataatgagc
     6301 gaaaatatct tcgccgccgc cttcagggca gatgaaacca aaccctttgg cattgttgaa
     6361 ccacttaaca gtaccctttt ccatgcttcg acatccttcg caaatcttat acaagtaaga
     6421 tggaataaac cggggtcaga gagggggctg ttcaaaacct cgccaactct agaaatacaa
     6481 tttagagaat tagggcgagc cgtcaagcat ttgacagggg ccagggggca ggtatgaatc
     6541 aaaaatttga agcagttaac gctattgaca ggaatgtgac agatgtcgct gatgccaacg
     6601 atagatgata gttatctatc atgtggagta gattggtcag gcaaataagc tcttgtcagc
     6661 ggcagggcgt tctgccgata accgtaaccg aagatgataa ctgacaatgg gtaaaacgaa
     6721 cgactggctg gactttgatc aactggcgga agaaaaagtt cgcgacgcgc taaaaccgcc
     6781 atctatgtat aaagtgatat tagtcaatga tgattacact ccgatggagt ttgttattga
     6841 cgtgttacaa aaattctttt cttatgatgt agaacgtgca acgcaattga tgctcgctgt
     6901 tcactaccag gggaaggcca tttgcggagt ctttaccgcc gaggttgcag aaaccaaagt
     6961 ggcgatggtg aacaagtacg cgagggagaa tgagcatcca ttgctgtgta cgctagaaaa
     7021 agcctgaatg caggcataaa aattggggga ggtgcctatg ctcaatcaag aactggaact
     7081 cagtttaaat atggctttcg ccagagcgcg cgagcaccgt catgagttta tgaccgtcga
     7141 gcacttgtta ctggcgctgc tcagtaaccc atctgcccgg gaggcgctgg aagcgtgttc
     7201 tgtggatttg gttgcgctcc gtcaggaact ggaagccttt attgaacaaa ccacacccgt
     7261 tctgcctgcc agtgaagagg agcgcgacac acagccgacg ctgagttttc agcgtgtact
     7321 gcaacgtgcg gtcttccatg tccagtcctc cggtcgcaat gaggttaccg gtgcaaacgt
     7381 tctggtcgct atctttagcg aacaggagtc gcaggcggca tatctgttgc gtaaacatga
     7441 agtcagccgt ctcgatgtgg tgaattttat ctctcatggc acgcgtaaag acgagccgac
     7501 acagtcttct gatcctggca gccagccaaa cagcgaagaa caagctggtg gggaggaacg
     7561 tatggagaat ttcacgacga acctgaatca gcttgcgcgc gtgggcggaa tcgacccact
     7621 gattggtcgt gagaaggagc tggagcgtgc tattcaggtt ctctgccgtc gccgtaaaaa
     7681 caacccgctg ctggtggggg aatctggtgt cggtaaaacc gcgattgcgg aaggtcttgc
     7741 ctggcgaatt gttcagggcg atgtgccgga agtgatggct gactgtacga tttactctct
     7801 cgatatcggt tctctgttag cgggcacaaa atatcgcggc gactttgaaa aacgttttaa
     7861 agcgttgctc aagcagctgg agcaggacac taacagcatc ctgtttattg atgagatcca
     7921 caccattatc ggtgcggggg cagcgtctgg tggtcaggtt gatgcggcta acctaatcaa
     7981 accgttgctc tccagcggta aaattcgtgt aattggttcg acaacctatc aggagttcag
     8041 caacattttc gagaaagacc gtgctctggc gcgtcgcttc cagaaaattg atattactga
     8101 accgtcaatc gaagaaactg ttcaaatcat caatggcctg aaaccgaagt atgaagcgca
     8161 ccacgacgtg cgttataccg caaaagcggt gcgtgcggcg gtagagctgg cggtgaaata
     8221 catcaatgat cgtcatctgc cggataaagc gattgatgtt atcgacgaag cgggcgctcg
     8281 cgcacgcctg atgccggtaa gcaaacgcaa gaaaaccgtt aatgtggcgg atattgagtc
     8341 cgtggtggcc cgtattgcac gcattccaga gaagagtgtt tctcagagtg accgcgatac
     8401 cctgaaaaac ctcggcgatc gcctgaaaat gctggtcttc ggtcaggata aagccattga
     8461 ggcgctgact gaagccatta agatggcgcg tgcaggttta ggtcacgaac ataaaccggt
     8521 cggttcgttc ctgtttgccg gtcctaccgg ggtcgggaaa acagaggtga cggtacagct
     8581 ttcgaaagcg ttgggcattg agcttctgcg ctttgatatg tccgagtata tggaacgcca
     8641 taccgtcagc cgtctgattg gtgcgcctcc gggatacgtt ggttttgatc agggcggttt
     8701 gctgaccgat gcggtaatca agcatccaca tgcggtgctg ctgctggacg aaatcgagaa
     8761 agcgcatccg gacgtgttca atattctgtt gcaggtgatg gacaacggta cgctgaccga
     8821 taacaacgga cgcaaagcgg acttccgtaa cgtggtgctg gtgatgacca ccaacgccgg
     8881 ggtacgggaa actgagcgca aatccattgg tcttatccac caggataaca gcaccgatgc
     8941 gatggaagag atcaagaaga tctttacacc ggaattccgt aaccgtctcg acaacattat
     9001 ctggtttgat catctgtcaa ccgacgtgat ccatcaggtg gtggataaat tcatcgtcga
     9061 gttgcaggtt cagctggatc agaaaggtgt ttctctggaa gtgagccagg aagcgcgtaa
     9121 ctggctggcc gagaaaggtt acgaccgggc aatgggcgct cgtccgatgg cgcgtgtcat
     9181 ccaggacaac ctgaaaaaac cgctcgccaa cgaactgctg tttggttcgc tggtggacgg
     9241 cggtcaggtc accgtcgcgc tggataaaga gaaaaatgag ctgacttacg gattccagag
     9301 tgcacaaaag cacaaggcgg aagcagcgca ttaatctaat tgttaggtag gttggtgaag
     9361 tccgtaatct cgaaagaggt tacggacttt ttgtttatgg ggtggaacgg tgaaaaccct
     9421 atttttggag gtgaaggtaa tttgttgata attagtgctg ctggaaaagg taaggataaa
     9481 aaagggtgac agcaggaaaa tgagatggtt ttgctttatt aacaacggac taaacgtgta
     9541 gtatttgagt tcactgccgt acaggcagct tagaaatcga cggggtgcgg taaaaccttt
     9601 gcgaacgcgt tcactgccgt acaggcagct tagaaattca caggtaacat actccacccg
     9661 cccaccatgt tcactgccgt acagaaagat aaaatgcgaa aaaaaagctc gcactttcgt
     9721 acgagctctt ctttaaatat ggcggtgagg gggggattcg aacccccgat acgttgccgt
     9781 atacacactt tccaggcgtg ctccttcagc cactcggaca cctcaccaaa ttgttttgct
     9841 accaaacctc atgggtggca acggggcgct actataggga gttggagtaa aacggtcaag
     9901 aagaatttta atgataatta ttgtttgctc atactgtaaa caagttgtgc agtatatcta
     9961 catcgagaca agttacggac ttatacttcc aaagtacttc atacatatca caaaataaaa
    10021 aggccggtta aaccgacctt ttactcgttc tttctcttcg cccatcaggc ggtaaaacaa
    10081 tcagcgacta cggaagacaa tgcggccttt gctcaggtcg tacggggtca gttcaacagt
    10141 cactttgtcg cccgtcagga tgcggatgta gtttttgcgc attttaccgg agatgtgtgc
    10201 agtaaccacg tgaccgtttt ctaactctac gcggaacatg gtattaggca acgtttcaag
    10261 aacggtacct tgcatttcaa tattgtcttc tttggccatc taatcctctg gggtatcact
    10321 accgtaattt gaaccggcaa gataatgccg aagttctgta aataagtaaa gatttgcgcg
    10381 ctaaatcgca acaaacaggt tcggcacatt actccgaaaa cacacggcta agccgcacca
    10441 aaagcgcaac gtataaggga gcggtgagat aaacgatggg cgttacctga cgcgaaaaat
    10501 tccttatcgg cagcggggta atgagcataa ccaactctgc gaccgcaatt ataacactct
    10561 ggggagaaat gtgccgaaaa cattcatttt tgtggtgaaa acaagcatcg tggcacccag
    10621 aaattattcg gcaatcgtcc gaggcgcatt tgattgagat aattcagata atcccgacgg
    10681 ggaatttcgc aggcaccaag cgatgctgtg tgatcgttaa ggacctggca gtcgataagc
    10741 ttaccgccat gactgataaa ttcctcacag aataccagaa gcgccgtttt agacgcattt
    10801 tccatccggc tgaacatgga ctcgccacaa aatagcgttc cctgggccac gccgtacata
    10861 ccgccgacaa gctcatcttc atgccagact tcaatggagt gggcatgacc gagttcgtga
    10921 aggcgatggt aggcttcgac cacgccacgc gtgatccagg ttccttcttc gcgatcgctg
    10981 gcacaacctt caatgacctg accaaaagcg taattcatcg tgacacgata gggcgagcgt
    11041 ttatgaaatc gcttcatact acggctgata tgcagtgatt caggccaaag caccgcgcga
    11101 ggatcgggcg accaccagag gatgggggcg cctggagaaa accacggaaa aataccacgc
    11161 tggtaagcca ttaacaggcg cgcagggcta agatcgcccc ccagagccag caggccgtta
    11221 ggctcacgta atgcgccttc cggggaaggg aaggctattg aatggcgaga aagctgaacc
    11281 aggcgcatga ccgcaaaact ccacgcaagt cggatcgttc aataatagct tacaaaccct
    11341 gcttaaactg gtaataacgc ccctgtctgg caagcagttc tgcgtgagta ccttgctcaa
    11401 taatttgccc gttgtccatc actattattt gttggaaacg agagagtccg cgaagtcgat
    11461 gggtgaccat taacaccgtt ttctcacgca tcatttctgc aagcaattca aggatctggc
    11521 tttcggttgt ggcatctaag ccttcggtag gttcatccag caacaccagt ggcgcatcat
    11581 gtaacagcgc acgggcgata gccagacggc gcagttcacc accggagagc tggcgtccgc
    11641 cttcacctaa ccaactgttg agacctgcgt cctcgagcag cttctccaga ccaacgcgac
    11701 gcaagatctc cgacagagcc tcatcactac tgccaggcga ggcgagtaac agattatcac
    11761 gcagcgtggc gctaaacaga tgcactcgct gaggaacaac gctgatggtc tgtcgtagag
    11821 ccgcttcatt caggctggct atggggctat cgttaagcaa aatctcgccc tgttgcgggt
    11881 cccaagcgcg ggtcagcagt tgtaacagtg ttgatttgcc gcatccggtt cgcccgagaa
    11941 tcgcaatatg ttccccggcg tttacctgaa gagaaatccc tttaagtgcc tgttgggatt
    12001 gctccggata agtgaactga acatcccgta atgtcagcga aacgcgatcg gcaacacgag
    12061 tttgggtatc agggaaggtg acttccggtt tttgatccgt taaatcagtg atccgaacgg
    12121 cagaggcaat gacttgcccc agatgctgaa atgcacccgt tactggtgcc agtgcttcaa
    12181 acgcggctaa cgcgcagaag acaaacaggg caattaacgc gccgggttga gcattgccgc
    12241 caacgccgcc agacgccatc cacagcatca ggatcaccgc taacgcgcca atgagcagca
    12301 ttatcgcttg cgacaatgcg gtcagttcag attgacggcg ttgcgcttcc agccattgaa
    12361 tttctgtatt ctctagttgc gtgcgataac gatcgctggc accaaaaatg gtcagctcag
    12421 cttgcccttg cagccaggcc gtcagttgtt ggcgatactg tccgcgaaga tgagtcagat
    12481 tttgcccggt gctttttccc gcacgataaa acagcggtgg catcaggaaa agcgtcagta
    12541 acataatgcc gcccagcgta aaggcgaggg tgaaatcaag gaaacttaac ccgactgtca
    12601 ccaccataat caccacaaaa gcgcccacca gcggcgagat aacgcgcagg taaagatgat
    12661 cgagcgtatc aacatccgcc accacgcgat tgagcaattc gccctgacga tagcgcgcca
    12721 gtccggcagg ggagaggggc aacaatttgc tgaaggtgta aatgcgcaga tgctgcaaca
    12781 cgcggaaagt cgcgtcgtga cttaccagac gttcaaaata acgcccggca gtacgggtga
    12841 ttgcggcacc acgcacgccc gcagcgggta gcatatagtt gaagctgtac agtccggcaa
    12901 cccccgcaac cgctgaggcc gagaggaacc agccggaaag tgtcaacaga ccgatactgg
    12961 cgagcagcgt cacaattgcc agcacaatac caagacttaa catccattta tgacgtttat
    13021 acagtgccag atagggtagc aaagcgcgca tttaaatctc ctcctgacga tgggccagta
    13081 atgtggcgaa tgggccacca gccacactta attccgcgta acgtccttgc tcaataatct
    13141 gaccatcctg cattacccaa atgacatccc agtcagcaag atcttctaac tggtgggtga
    13201 ccattaacgt tgtctggcgc agagaggcgg cattcagcgc ctccattacg cgctgttcac
    13261 tgtgagcatc aaggctggca gcgggttcat ccaacagtaa tagcgaacag ggatttagta
    13321 acgcacgggc caccgccacg cgctgcgcct gccccacgga aaggcgggca gcctggtcgc
    13381 caacaggcgt atcaacgcct tgtggcagga gcggtagaaa ctcgctgacc caggcgttat
    13441 ccagcgctgc ttgtaattca tgttcgctgg catcaggtcg cgccagtagt acgttatccc
    13501 gtaatgttgc tgccggtaat tgtggatttt gcccaaccca ggagagatgt ttacgccatg
    13561 attctggtga taaatcgcgt aattctatcc cgttgattcg tagcgatccc tgatatgaga
    13621 gaaaaccaga aagcgcgttc aacagtgaac ttttacctga accgctgcga ccaaccaaca
    13681 cggcacgttg gcctgctggc aaagtaaagt tcagcggtcc ggccagcgtt ttaccttccg
    13741 gcgacgtgat aaacagatcc tcggcctcaa tggtcaccgg atcggtcgat gctaattcca
    13801 cctcgccacg ctgcggatgg gcgagcgggg tttccataaa cgttttcaga ctgtcagctg
    13861 caccaacagc ctgggcttta gcatgataaa acgtaccgag atcgcgtaat ggctggaaaa
    13921 actctggcgc aaggatcagg gccagaaaac ccgcagccag cgtcacgcca gtatcgtagt
    13981 gaccaaaatc cagctcgccg agataggaaa aaccaaagta gaccgccacc agagcaattg
    14041 acagcgaggt aaaaaattcg agaatgccgg aggataaaaa tgccagccgt agcacttcca
    14101 ttgtccgttg gcggaaatct tccgaagcag aacgaatact ttcaatttca gcttcaccac
    14161 gaccaaaaat acgcaatgtt tccatgccgc gcaggcgatc gaggaaatgc ccacttaagc
    14221 gagcaagagc gagaaagtta cgtcggttag catcggcagc ccccattcca accagcgcca
    14281 taaacaacgg aattaacggt gcagtgccca gcagaatgag cgccgcagcc cagtttgagg
    14341 ggaagattgc caccacaatc agcaacggca ccgacactgc cagcgccatt tgcggcaggt
    14401 agcgtgcata gtaatcatgc atatcgtcaa tttgctcgag taccagcgtc gcccagctcc
    14461 ccgcaggttt accctgaatc cacgctggcc ctgcttgttg cagacggtcg agaacctgac
    14521 ggcggatggc aaagcggata tgctgcccgg cgtgataacc cacccgttcg cgtaaccaga
    14581 ccgcccatgc gcgcagtaca aaggtcagaa ccagtaacgt aaagggaagc agcagggctt
    14641 cacggggaat attctccata atcatatgtt gcagaattcg cgccatgaac caggcctggg
    14701 caatgatcaa tatgccgctt acaaagccca gcagacgaga aatattcagc caacgttggg
    14761 agatgacgct ttgctgtttt aaccagcggg ttaactcttt ttgacgagat ttattcattg
    14821 cacgcttagc aggtgagtta tcagaattat ttgcagagca atgttacaac ggggaaaaaa
    14881 taaaggcgac ccatagtcgc aaggtgtcgc cttctttact tttgttactg atttgtaaaa
    14941 ttattttgcg tcagctaaac catcgaggta gcgttccgca tcaagtgctg ccatgcagcc
    15001 agtaccggcc gaagtaatgg cctggcgata aatgtgatcc atcacgtcgc ctgcggcaaa
    15061 gacgccagga atgctggtct gggtggcatt accatgaata cccgactgta ctttgatgta
    15121 gccgttttcc agttccagct gcccttcgaa aatcgcagta ttcgggctgt gaccgatagc
    15181 aacaaacaga ccggcaacgt cgagtgactc gatgttatcg ctgttttgcg tatcgcgcag
    15241 acgaacgcca gtgacaccca tttgatcgcc ggtcacttct tccagcgtac ggttggtgtg
    15301 cagaatgatg ttgccgttct ccactttatc catcagacgc ttaataagga ttttttccgc
    15361 gcggaaaccg tcacggcggt gaatcagatg cacttccgaa gcgatgttag acagatacag
    15421 cgcctcttca accgcggtat tgccgccgcc gatgaccgca actttctggt tgcgatagaa
    15481 gaaaccgtcg caggttgcac aagcagaaac cccacggcct ttaaaggctt cttcagaggg
    15541 caggccgaga tatcgtgcag aagctccggt ggcaataatc agcgcgtcgc aagtgtattc
    15601 gccgttatcg ccattcagac ggaacggacg gttttgcaga tccaccttgt tgatatgatc
    15661 aaaaatgatc tcagtttcaa acttggtggc atgttcgtgc atgcgctcca ttaataacgg
    15721 accggtcaga tcgtttggat caccaggcca gttttccact tccgtggtgg tggtcagttg
    15781 gccgcctttt tccatgccgg taatcagcac aggttgcagg ttggcgcgcg ccgcgtagac
    15841 agcagcggtg tatcccgccg ggcctgaacc caggataagc agtttactgt gtttggtcgt
    15901 gcccatgaga tccccatagt tgttggcaga caatgggcag gattgtaggg aatttacaga
    15961 cgtaaaaaaa gagtatgacg attttgttaa caatttgtgc aatcggcagc atcgataagc
    16021 aggtcaaatt ctcccgtcat tatcacctct gctacttaaa tttcccgctt tataagccga
    16081 ttaaatgatg aataaacgcc cctgttaatg aatatctggc atgttgtact aaaaatcgat
    16141 gttttgcttt gacaatcccc tggtgttttg cgaaaacatt cgaggaagaa aaaaaacagt
    16201 attcttatat gcgcataacc atgcatgtaa ataccatgtt taccgtgcta gtgaaatcta
    16261 cgtatggcgt ggacagacgc cattcgtgat gtcgatagct gccacaaggc aacggtcttc
    16321 tcaccgtaga cccaggcatt gcgcgccgtg aatcttcatg atttcggtct atcgtgacgg
    16381 gtagcgactc tgaacagtga tgtttcaggg gcagacagga gtagggaagg aatacagaga
    16441 gacaataata atggtagata gcaagaagcg ccttggcaaa gatctcgacc gtatcgatcg
    16501 taacattctt aatgagttgc aaaaggatgg gcgtatttct aacgtcgagc tttctaaacg
    16561 tgtgggactt tccccaacgc cgtgccttga gcgtgtgcgt cggctggaaa gacaagggtt
    16621 tattcagggc tatacggcgc tgcttaaccc ccattatctg gatgcatcac ttctggtatt
    16681 cgttgagatt actctgaatc gtggcgcacc ggatgtgttt gaacaattca ataccgctgt
    16741 acaaaaactt gaagaaattc aggagtgtca tttagtatct ggtgatttcg actacctgtt
    16801 gaaaacacgc gtgccggata tgtcagctta ccgtaagttg ctgggggaaa ccctgctgcg
    16861 tctgcctggc gtcaatgaca cacggacata cgttgttatg gaagaagtca agcagagtaa
    16921 tcgtctggtt attaagacgc gctaacacgg aacaggtgca aaatcggcgt attttgatta
    16981 cactcctgtt aatccataca gcaacagtac tggggtaacc tggtactgtt gtccgtttta
    17041 gcatcgggca ggaaaagcct gtaacctgga gagcctttct tgagccagga atacactgaa
    17101 gacaaagaag tcacattgac aaagttaagt agcggccgcc gccttctgga ggcgttgctg
    17161 atccttattg tcctgtttgc cgtctggttg atggctgcct tactaagctt taacccttcg
    17221 gaccccagct ggtcgcaaac ggcctggcat gaacctatcc ataatttagg tgggatgccc
    17281 ggtgcgtggt tggcagatac gctgttcttt atttttggcg tgatggctta caccattccc
    17341 gtcattattg tcggcggttg ttggtttgcc tggcgtcatc agtccagcga cgaatacatt
    17401 gattattttg ccgtttcgct acgcatcatt ggcgttttgg cgctcatcct tacctcctgt
    17461 ggtctggcgg caatcaacgc tgacgatatc tggtattttg cctccggtgg cgtcattggc
    17521 agcttactaa gcactacgct acaaccactg ctacacagta gcgggggaac tattgcgctg
    17581 ctctgcgttt gggcagcggg cctgacgttg ttcaccggtt ggtcatgggt gaccattgct
    17641 gaaaaactcg gcggctggat tttaaacatt ctcaccttcg ccagtaatcg tacccgtcgc
    17701 gatgatacct gggtcgatga agatgagtat gaagacgacg aagagtatga agatgaaaat
    17761 cacggcaaac agcatgaatc acgccgtgcc cgtattcttc gcggcgcgct agcgcgtcgt
    17821 aaacggttgg cggaaaaatt cattaatccg atggggcggc aaacagacgc tgcgttgttc
    17881 tccggtaagc ggatggatga tgacgaagag attacctaca ctgcacgcgg tgtggctgcc
    17941 gaccctgacg acgtcctatt ttcgggcaat cgtgcaacgc agccagaata tgacgaatac
    18001 gatccattat taaacggtgc gccaattacc gaacctgtcg ctgtagcagc tgctgctacc
    18061 acggcggcac aaagctgggc tgcgccggtt gaacctgtga ctcagacgcc gcctgttgcc
    18121 tctgttgatg ttccaccttc gcaacctaca gtagcctggc agcctgtacc gggtccacaa
    18181 acgggagagc cggttattgc tcctgcaccg gaaggttacc cacagcagcc acaatatgcg
    18241 cagcctgcag tgcaatataa tgagccgctg caacaaccag tacagccaca gcagccgtat
    18301 tatgcacctg cagctgaaca acctgtgcaa caaccttact acgctcctgc ggcggaacaa
    18361 cctgttcagc aaccgtatta cgcccctgcg ccagaacaac cggtggcagg taacgcctgg
    18421 caagccgaag agcagcaatc cacttttgct ccacagtcta cataccagac tgagcaaact
    18481 tatcagcaac cagccgctca ggagccgttg taccaacagc cgcaacccgt tgaacagcag
    18541 cctgttgtgg agcctgaacc cgttgtagaa gagacaaaac ccgcgcgtcc gccactttac
    18601 tattttgaag aagtggaaga gaagcgagcc cgtgaacgtg aacaacttgc ggcctggtat
    18661 caaccgattc cagaaccggt taaagaacca gaaccgatca aatcttcgct gaaagcacct
    18721 tctgttgcag cagtacctcc agtagaaacc gctgccgctg tttctccgct ggcatctggc
    18781 gtgaaaaaag cgacactggc gacgggggct gccgcaaccg ttgccgcgcc agtcttcagt
    18841 ctggcaaata gcggtggacc gcgtccgcag gtcaaagagg ggattggccc gcagttgcca
    18901 cgaccgaaac gtatccgcgt gccaactcgc cgtgaattgg cgtcttacgg tattaagctg
    18961 ccctcacagc gtgcggcgga agaaaaagcc cgtgaagccc agcgcaatca gtacgattct
    19021 ggcgatcagt acaacgatga tgaaatcgat gcgatgcagc aggatgaact ggcacgtcag
    19081 ttcgcccaga cacagcagca acgctatggc gaacagtatc aacatgatgt gcccgtaaac
    19141 gcagaagatg cagatgctgc ggcagaggct gaactggctc gtcagtttgc gcaaactcaa
    19201 caacaacgtt attccggcga acaaccggct ggggcgaatc cgttctcgct ggatgatttt
    19261 gaattttcgc caatgaaagc gttgctggat gatggtccac acgaaccgtt gtttacgcca
    19321 attgttgaac ctgtacagca gccgcaacaa ccggttgcac cgcagcagca gtatcagcag
    19381 ccacaacagc cggttgcgcc acagcagcaa tatcagcagc cgcaacagcc ggttgcgccg
    19441 cagccacaat atcagcagcc gcaacaaccg gttgcgccac agcagcaata tcagcagccg
    19501 caacaaccag ttgcgccgca gccgcagtat caacagccgc aacaaccggt tgcgccgcag
    19561 cagcagtatc agcagccaca acagccagtt gcgccacaac cgcaggatac cctgcttcat
    19621 ccgctgttga tgcgtaatgg cgacagccgt ccgttgcata aaccgacgac gccgctgcct
    19681 tctctggatt tgctgacacc gccgccgagc gaagtggagc cggtagatac ctttgcgctt
    19741 gaacaaatgg ctcgcctggt ggaagcgcgt ctggctgatt tccgtattaa agccgatgtt
    19801 gtcaattact ctccggggcc ggttatcact cgctttgaat tgaacctggc accgggcgta
    19861 aaagcggcgc gcatttctaa cttgtcacgg gaccttgccc gttcactttc gacggtggcg
    19921 gtgcgtgtcg ttgaagttat tcctggcaaa ccctatgtag gtctggagtt accgaataaa
    19981 aaacgacaaa ccgtttatct gcgcgaagtt ttggataacg ccaaattccg cgataatccg
    20041 tcgccattaa ccgtggtgct gggtaaagat atcgccggtg agccggtggt tgccgatctg
    20101 gcgaaaatgc cgcacttgtt ggttgcgggg actaccggtt ccggtaaatc tgtcggtgtg
    20161 aacgcgatga tcctgagcat gctttataaa gcacagccag aagatgtgcg tttcatcatg
    20221 atcgacccga aaatgctgga gctttcggtt tatgaaggca ttccgcatct gttaacggaa
    20281 gtcgttactg atatgaaaga tgccgccaac gcgctgcgct ggtgtgttaa cgagatggag
    20341 cgtcgttata aactgatgtc tgcgctgggt gtgcgtaatc tggcgggtta taacgaaaaa
    20401 attgctgaag ccgatcgcat gatgcgtccg attccagacc cgtactggaa gccgggtgac
    20461 agtatggatg cccagcatcc ggtgctgaaa aaagaaccat acattgtggt gttggttgac
    20521 gaatttgccg acctgatgat gacggtaggt aaaaaagtgg aagagctgat agcacgtctg
    20581 gcgcaaaaag cccgtgccgc gggtatccac ctcgtactgg caactcagcg tccatcggtt
    20641 gatgttatta ctggtctgat taaagcgaat attccgaccc gtatcgcctt taccgtatcc
    20701 agtaagattg actcacgtac cattcttgat caggctggcg cggaatcact gctggggatg
    20761 ggggatatgc tctactctgg gccgaactcc acgttgccgg tacgtgtcca tggtgctttt
    20821 gttcgcgatc aggaagttca tgccgtggtg caggactgga aagcgcgtgg tcgcccacag
    20881 tatgttgatg gcatcacttc cgacagtgaa agcgaaggtg gcgcgggcgg tttcgatggc
    20941 gctgaagaac tggatccgtt gttcgatcag gcggtgcagt ttgtcactga aaaacgcaaa
    21001 gcgtcaattt ctggcgtaca gcgtcagttc cgcattggtt ataaccgtgc agcgcgtatt
    21061 atcgaacaga tggaagcgca ggggattgtc agcgaacagg ggcacaacgg taatcgtgaa
    21121 gtgctggctc caccgccgtt tgactaatta atgcattgcc ggataaggcg cggtagcgtc
    21181 gcatccggca ctctatcaac tgaaaattca gtattttctt ctttcctcaa gctgattatt
    21241 agcctggaat agagagtaga gggaactccc gatcgggagt gacgtaattt gaggaataat
    21301 gatgaaaaaa attgccatca cctgtgcatt actctcaagc ttagtagcaa gcagcgtttg
    21361 ggctgatgcc gcaagcgatc tgaaaagccg cctggataaa gtcagcagct tccacgccag
    21421 cttcacacaa aaagtgactg acggtagcgg cgcggcggtg caggaaggtc agggcgatct
    21481 gtgggtgaaa cgtccaaact tattcaactg gcatatgaca caacctgatg aaagcattct
    21541 ggtttctgac ggtaaaacac tgtggttcta taacccgttc gttgagcaag ctacggcaac
    21601 ttggctgaaa gatgccaccg gtaatacgcc gtttatgctg attgcccgca accagtccag
    21661 cgactggcag cagtacaata tcaaacagaa tggcgatgac tttgtcctga cgccgaaagc
    21721 cagcaatggc aatctgaagc agttcaccat taacgtggga cgtgatggca caatccatca
    21781 gtttagcgcg gtggagcagg acgatcagcg cagcagttat caactgaaat cccagcaaaa
    21841 tggggctgtg gatgcagcga aatttacctt caccccgccg caaggcgtca cggtagatga
    21901 tcaacgtaag tagaggcacc tgagtgagca atctgtcgct cgatttttcg gataatactt
    21961 ttcaacctct ggccgcgcgt atgcggccag aaaatttagc acagtatatc ggccagcaac
    22021 atttgctggc tgcggggaag ccgttgccgc gcgctatcga agccgggcat ctgcattcta
    22081 tgatcctctg ggggccaccg ggtaccggca aaacaaccct cgctgaagtg attgcccgct
    22141 atgcgaacgc tgatgtggaa cgtatttctg ccgtcacctc tggcgtgaaa gagattcgcg
    22201 aggcgatcga gcgcgcccgg caaaaccgca atgcaggtcg ccgcactatt ctttttgttg
    22261 acgaagtgca ccgattcaac aaaagccagc aggatgcatt tctgccacat attgaagacg
    22321 gcaccatcac ttttattggc gcaaccactg aaaacccgtc gtttgagctt aattcggcac
    22381 tgctttcccg tgcccgtgtc tatctgttga aatccctggg tacagaggat attgagcaag
    22441 tactaactca ggcgatggaa gacaagaccc gtggttatgg tggtcaggat attgttctgc
    22501 cagatgaaac acgacgcgcc attgctgaac tggtgaatgg cgacgcgcgc cgggcgttaa
    22561 atacgctgga aatgatggcg gatatggccg aagtcaatga tagcggtaag cgggtcctga
    22621 agcctgaatt attgaccgaa atcgccggtg agcgaagcgc ccgctttgat aacaaaggcg
    22681 atcgctttta cgatctcatt tccgcactgc ataagtcggt acgtggtagc gcacccgatg
    22741 cggcgctcta ctggtatgcg cgaattatta ccgctggtgg cgatccgtta tatgtcgcgc
    22801 gtcgttgcct ggcaattgct tccgaagatg tcggcaatgc cgacccacgg gcgatgcagg
    22861 tggcaattgc ggcctgggat tgctttactc gcgttggccc ggcggaaggt gaacgcgcca
    22921 ttgctcaggc gattgtttac ctggcctgcg cgccaaaaag caacgctgtc tacactgcgt
    22981 ttaaagctgc gctggcggat gctcgcgaac gcccggatta tgacgtgccg gttcatttgc
    23041 gtaatgcgcc gacgaaatta atgaaggaaa tgggctacgg gcaggaatat cgttacgctc
    23101 atgatgaagc aaacgcttat gctgccggtg aggtttactt cccgccggaa atagcacaaa
    23161 cacgctatta tttcccgaca aacaggggcc ttgaaggcaa gattggcgaa aagctcgcct
    23221 ggctggctga acaggatcaa aatagcccca taaaacgcta ccgttaatgt tatcgttgcg
    23281 gtaatgttgt tactgtatcc ctgtggtcgc aggctgtggc cacatctccc atttaattcg
    23341 ataagcacag gataagcatg ctcgatccca atctgctgcg taatgagcca gacgcagtcg
    23401 ctgaaaaact ggcacgccgg ggctttaagc tggatgtaga taagctgggc gctcttgaag
    23461 agcgtcgtaa agtattgcag gtcaaaacgg aaaacctgca agcggagcgt aactcccgat
    23521 cgaaatccat tggccaggcg aaagcgcgcg gggaagatat cgagccttta cgtctggaag
    23581 tgaacaaact gggcgaagag ctggatgcag caaaagccga gctggatgct ttacaggctg
    23641 aaattcgcga tatcgcgctg accatcccta acctgcctgc agatgaagtg ccggtaggta
    23701 aagacgaaaa tgacaacgtt gaagtcagcc gctggggtac cccgcgtgag tttgactttg
    23761 aagttcgtga ccacgtgacg ctgggtgaaa tgcactctgg cctcgacttt gcagctgcag
    23821 ttaagctgac tggttcccgc tttgtggtaa tgaaagggca gattgctcgc atgcaccgcg
    23881 cactgtcgca gtttatgctg gatctgcata ccgaacagca tggctacagt gagaactatg
    23941 ttccgtacct ggttaaccag gacacgctgt acggtacggg tcagctgccg aaatttgctg
    24001 gcgatctgtt ccatactcgt ccgctggaag aagaagcaga caccagtaac tatgcgctga
    24061 tcccaacggc agaagttccg ctgactaacc tggtgcgcgg tgaaatcatc gatgaagatg
    24121 atctgccaat taagatgacc gcccacaccc catgcttccg ttctgaagcc ggttcatatg
    24181 gtcgtgacac ccgtggtctg atccgtatgc accagttcga caaagttgaa atggtgcaga
    24241 tcgtgcgccc ggaagactca atggcggcgc tggaagagat gactggtcat gcagaaaaag
    24301 tcctgcagtt gctgggcctg ccgtaccgta aaatcatcct ttgcactggc gacatgggct
    24361 ttggcgcttg caaaacttac gacctggaag tatggatccc ggcacagaac acctaccgtg
    24421 agatctcttc ctgctccaac gtttgggatt tccaggcacg tcgtatgcag gcacgttgcc
    24481 gcagcaagtc ggacaagaaa acccgtctgg ttcataccct gaacggttct ggtctggctg
    24541 ttggtcgtac gctggttgca gtaatggaaa actatcagca ggctgatggt cgtattgaag
    24601 taccagaagt tctgcgtccg tatatgaacg gactggaata tattggctaa tacccaattt
    24661 ttctgaatct aaaaagcgcc tgcgggcgct ttttttgtct ccctttgata ccgaacaata
    24721 attactcctc acttacacgt aatactactt tcgagtgaaa atctacctat ctctttgatt
    24781 ttcaaattat tcgatgtata caagcctata tagcgaactg ctatagaaat aattacacaa
    24841 tacggtttgt tactggaatc aatcgtgagc aagcttgagt gagccattat gaaaacgaaa
    24901 atccctgatg cggtattggc tgctgaggtg agtcgccgtg gtttggtaaa aacgacagcg
    24961 atcggcggcc tggcaatggc cagcagcgca ttaacattac cttttagtcg gattgcgcac
    25021 gctgtcgata gcgccattcc aacaaaatca gacgaaaagg ttatctggag cgcctgtaca
    25081 gttaactgtg gtagtcgctg cccgctacgt atgcacgtcg tggacggtga aatcaaatat
    25141 gtcgaaacgg acaataccgg cgatgacaat tacgacggcc tgcaccaggt tcgcgcctgc
    25201 ctgcgtgggc gttccatgcg tcgccgtgtc tacaatccgg accgcctgaa atatccgatg
    25261 aaacgagtcg gggcgcgcgg tgaaggcaaa ttcgagcgca ttagctggga agaagcctac
    25321 gacatcatcg cgaccaatat gcagcgcctg atcaaagagt acggcaacga gtctatctat
    25381 ctgaactatg gcaccggtac gctgggcggc accatgaccc gctcctggcc gccgggaaat
    25441 accctggtcg cgcggctgat gaactgctgc ggcggctatc tgaaccatta cggcgactac
    25501 tcctccgcgc aaattgcgga aggtttgaac tatacctacg gcggctgggc agatggcaac
    25561 agcccgtcgg atatcgaaaa cagtaagctg gtagtgctgt ttggtaataa ccctggcgaa
    25621 acgcgaatga gtggcggtgg ggtgacttac tatcttgaac aggcacgcca gaaatctaat
    25681 gcccgcatga tcatcatcga tccgcgctat accgacaccg gtgccgggcg cgaagatgag
    25741 tggatcccta ttcgtccggg aacagatgcc gcactggtta acggtctggc gtacgtcatg
    25801 atcactgaaa acctggtgga tcaggcattc ctcgataaat attgcgttgg ctacgatgag
    25861 aaaaccctgc cagccagtgc gccgaaaaat ggccactata aagcttatat tctgggtgaa
    25921 gggccagatg gcgtggctaa aacaccggaa tgggcctcac aaatcaccgg tgttccggca
    25981 gacaaaatca tcaaactggc tcgtgaaatc ggcagtacca aaccggcgtt tatcagccag
    26041 ggatggggcc cgcagcgtca cgctaacggt gaaatcgcaa cccgtgctat ctcgatgctg
    26101 gcgattctga ccggtaacgt tggtattaat ggagggaaca gcggcgcgcg tgaaggttca
    26161 tacagcttac cgtttgtccg tatgccgacc ttggaaaacc cgatccagac cagcatttcg
    26221 atgtttatgt ggaccgatgc cattgaacgt ggcccggaaa tgacggcgct gcgtgatggt
    26281 gtacgcggga aagataagct ggatgtgccg atcaaaatga tctggaacta tgccggtaac
    26341 tgcctggtta accagcattc tgaaatcaac cgtacccatg aaatccttca ggatgataag
    26401 aagtgcgagc tgattgtggt tatcgactgc cacatgacct catcggcgaa atatgctgac
    26461 atcctgctgc ctgactgcac cgcttccgaa cagatggact ttgcgctgga tgcatcctgc
    26521 gggaatatgt cttacgtgat tttcaacgat caggtgatta aaccgcgctt tgaatgtaag
    26581 accatctatg aaatgaccag cgaactggca aaacgtcttg gcgttgagca acagtttact
    26641 gaaggccgta cccaggaaga gtggatgcgg catctgtatg cccagtcgcg ggaagcgatt
    26701 cctgaactgc caacgtttga agagttccgc aagcagggga tctttaaaaa gcgcgaccca
    26761 caagggcatc acgttgctta taaagccttc cgtgaagatc cgcaggcaaa cccactgact
    26821 acgccatcgg gcaaaattga gatttattcg caggcgctgg ctgacattgc cgctacctgg
    26881 gaattgccag aaggcgatgt gatcgatcca ctgccgatct acacgccggg ctttgaaagt
    26941 tatcaggatc cgctgaacaa acagtatccg ctgcagctta cgggtttcca ctataaatct
    27001 cgcgttcact caacttacgg caacgttgat gtgctgaaag cagcttgccg tcaggaaatg
    27061 tggatcaacc cgcttgatgc gcaaaaacgc ggtatcaaca acggcgataa agtcaggatc
    27121 tttaacgatc gtggtgaagt tcatattgag gcgaaagtga cgccacgaat gatgccaggt
    27181 gtggtcgcac tgggtgaagg tgcctggtat gacccggatg caaaacgtgt cgataagggt
    27241 ggttgtatta acgtactgac cactcaacgt ccgtctcctc tcgctaaggg gaatccgtca
    27301 catacaaacc ttgttcaggt tgaaaaggtg taaggagtaa ccgatgacaa cccagtatgg
    27361 attttttatt gattccagcc gttgcaccgg ttgcaaaacc tgcgagctgg cttgtaaaga
    27421 ctacaaagat ttgacgccag aagtcagctt ccgccgcatt tatgaatatg ctggcggcga
    27481 ctggcaggaa gataacggtg tctggcacca gaacgtgttt gcctactatc tgtcgatttc
    27541 gtgcaaccac tgcgaagatc cggcttgtac taaagtctgc ccgagcggtg cgatgcataa
    27601 acgtgaagat ggttttgttg tggtcgatga agatgtgtgc attggctgcc gctattgcca
    27661 tatggcttgc ccgtatggcg caccgcaata taacgaaacg aaaggccata tgaccaaatg
    27721 cgatggttgt tatgaccgtg ttgcggaggg taaaaagccg atctgtgttg aatcttgtcc
    27781 gctgcgggcg ctggatttcg gccctatcga cgagctgcgt aaaaaacatg gcgatctggc
    27841 ggccgttgcg ccgttgccgc gagctcactt taccaaaccg aatattgtga tcaaacccaa
    27901 tgccaatagc cgcccgaccg gggataccac tggctatctg gcaaacccga aggaggtgtg
    27961 agatgggaag tggatggcat gaatggccgc tgatgatctt cacggtcttc gggcaatgtg
    28021 tggcgggtgg ttttatcgtt ctggctttgg cgctgctcaa aggcgacctg cgagcagaag
    28081 cccagcagcg tgttatcgcc tgcatgtttg gcttatgggt gctgatgggc attggcttta
    28141 tcgcttctat gctccatctt ggttcaccga tgcgcgcttt taactcgctc aaccgggtag
    28201 gggcttcagc actcagtaac gaaatcgcca gcggttcgat cttttttgcc gtaggcggca
    28261 tcggctggtt gctggcaatg ctgaaaaagc tgtcaccggc attgcgtacg ctttggctgg
    28321 tggtgacgat ggttcttggc gtcgtctttg tctggatgat ggtgcgtgtg tataacagca
    28381 ttgataccgt tccgacctgg tacagcatct ggacgccgat gggcttcttc ctgacgatgt
    28441 tcatgggcgg cccgttactg gggtacctgt tgctgagtct ggctggcgtc gatagctggg
    28501 cgatgcgtct gctgccagcc atttccgtac tggcactggt ggtgagtggc gtggtgtcag
    28561 tgatgcaggg cgcagagctg gcgactattc atagttctgt gcagcaggcc gcagcgctgg
    28621 tgccggacta tggtgcgctg atgtcctggc ggatcgtgct tttggccgtt gccctgtgct
    28681 tgtggattgc accacagcta aaaggttatc agcctgcggt tccgctacta tccgtctcat
    28741 tcattctgct tctggcaggg gaattaatcg gtcgcggcgt attctatggt ttgcatatga
    28801 ccgtggggat ggccgtcgca agctaatcag taacaaccgg ggtttcggcc ccggttctct
    28861 tttatttctg cttcgttaac gtgtcataac tggtcatcaa attacgataa tcaggaatat
    28921 ggttagagaa caacgtcgcc agtccttcaa tatcattacg ccagtcacgg tgcagctcgc
    28981 aagccacgcc gaaccatgtc atcaattgcg cacctgcttg cgacatgcga tcccatgccg
    29041 aatgccgggt aatttcatta aaggtccctg acgcgtcagt cacaacaaac acatcaaacc
    29101 cttcttcaat ggctgaaagc gccgggaatg ccacgcaaac ttcggttacc acaccggcaa
    29161 taattaactg ttttttacct gtcgctttga ctgcttttac aaaatcttcg ttatcccagg
    29221 cgttaatatt gcccgggcga gcaatgtaag gtgcatcggg gaattgtgct ttcagttctg
    29281 gaactaatgg gccgttagga ccggtttcaa aactggtggt aagaatagtt ggtaagttga
    29341 aatacttagc caggtcaccc agcgccagca cgttattttt aaacttatcg ggttcgatat
    29401 cccgtacaag ggaaagtaaa ccagcctggt gatcaacaag caaaacagca gcatcatttt
    29461 tatcaagacg aacatacggt ttggtcatcc tcttctcctt tcgagagtca gtggtacgcc
    29521 tgaaatcggt cagacgtgat gcattgctct gaaagcatag acgggaaata tgagtttgct
    29581 gtgaccatga aatttttcga ctgaacgaag cgtatgaaat ttgtgttaga tcaataaaaa
    29641 caatcagatg agattaatca tctcttcagt attagcgtta tgaatgagta atataaatcg
    29701 ataataccgc cgttgcgata tgcgcgtatt ttcacccatt gacaatgttt ttggcggtgg
    29761 catgatgcgc atgaaatttg aacttcctca cggttttaat tcatgtccac gtatacccgg
    29821 cctgtcatgc ttttgctgtc tggcctgctt ttgttgactc tggcgatcgc ggtgttaaat
    29881 acactcgtgc cgctttggct cgcccaggaa cacatgtcca catggcaggt aggcgttgtc
    29941 agctcatcct attttaccgg caaccttgtc ggtacattgc tgacagggta tgtcattaaa
    30001 cgcattggtt ttaaccgcag ctattatctg gcctccttca tttttgccgc tggctgtgcc
    30061 ggccttggcc tgatgattgg attctggagt tggttggcgt ggcgttttgt cgcgggcgtc
    30121 ggctgtgcca tgatttgggt agtcgttgag agcgcgctga tgtgcagtgg gacgtcacgt
    30181 aaccgtgggc gtttgcttgc tgcatacatg atggtttatt acgtgggaac gtttttaggc
    30241 cagttactgg tcagcaaggt ctcaaccgag ctgatgagcg tattgccgtg ggttacaggt
    30301 ttgacattgg cagggatctt accgctgttg tttacgcgtg tcctgaatca gcaggctgaa
    30361 aaccatgatt cgacgtcaat tacggcaatg ctaaaactcc gtcaggcgcg gcttggcgtg
    30421 aatggctgca ttatctcagg aatcgttctg ggatctctat atggcctgat gcctctgtac
    30481 ctcaatcaca aaggggtgag caatgccagt attggtttct ggatggcggt actggtcagt
    30541 gcgggtatcc tcggacaatg gccgattgga cgtctggcgg ataagtttgg tcgactgctg
    30601 gttttgcgtg ttcaggtctt tgtcgtcatt ctcggcagta ttgcgatgct tagccaggcg
    30661 gcgatggccc cagcgttatt catcctcggt gccgctggct ttacgctata tccggtggcg
    30721 atggcatggg cttgcgagaa agttgaacat catcaactgg tggcgatgaa ccaagcctta
    30781 ctgttgagct atactgtggg aagtctgctt ggcccgtcat ttaccgctat gctaatgcag
    30841 aatttctccg ataatttatt gtttatcatg atcgccagcg tatcgtttat ctatttgctg
    30901 atgctgctgc gcaacgccgg tcatacgccg aaacccgttg ctcacgtgta aatgaattca
    30961 agcagagtgt gaacttactg tttcacactc tgcttttttg tttcttctat ctgacttgct
    31021 ttattccaaa ttttattcgt ttaaaaataa aatgtgtagc aggttataat tttgcatttc
    31081 gctatttccg cacttcttat ttgccgcgca taatccctcg ttttacagat gcccctataa
    31141 ttttggcgaa ggatttgtct atggctggga atgttcagga aaaacagctg cgatggtaca
    31201 acattgcgct gatgtctttt atcactgtct ggggttttgg caacgttgtt aataactacg
    31261 ccaaccaggg gctggtggtt gttttttcat gggtgtttat ctttgcgctc tatttcacac
    31321 cttatgcgct aattgttggt cagttaggct cgaccttcaa agatgggaag ggcggggtca
    31381 gtacctggat taaacacacg atgggacccg gactggctta tctcgccgcg tggacctact
    31441 gggtggtgca tattccctat ctggcacaaa aaccccaggc aattctgatt gcgctcggtt
    31501 gggcgatgaa aggcgacggt tcgctaatca aagaatattc agtcgtagcg ttacaggggt
    31561 taacgctggt gctgtttatc ttctttatgt gggttgcttc acgcggtatg aaatcgctga
    31621 aaatcgtcgg ttctgtggca gggattgcta tgtttgttat gtcgctcctg tatgtggcga
    31681 tggcggtaac cgcgcctgca attactgaag tgcatattgc gaccacaaac attacctggg
    31741 aaacgttcat tcctcatatc gactttacct acattaccac tatttcaatg ctggttttcg
    31801 cggttggcgg agcagagaag atttctcctt acgttaatca aacgcgcaac ccaggaaaag
    31861 aatttccaaa agggatgtta tgcctggcgg tgatggttgc ggtttgtgcc attctgggct
    31921 cgctggcgat ggggatgatg tttgattcgc gtaatatccc ggatgactta atgaccaacg
    31981 gtcagtatta cgcctttcag aagctgggcg agtattacaa catgggtaat actttaatgg
    32041 tgatttacgc cattgcgaat accctgggac aagtagcggc gctggtattc tcgattgatg
    32101 ccccgcttaa agtgctatta ggtgatgctg acagcaaata tattccagcc agtttatgtc
    32161 gtaccaacgc ttctggtacg cccgttaatg gctattttct gaccctggta ctggtggcga
    32221 ttctgattat gctgccgact ctcggcattg gtgatatgaa caatctctat aaatggctgt
    32281 tgaaccttaa ttcggtagtg atgccgctgc gttatttatg ggtatttgtt gcatttattg
    32341 cagtcgttcg cctggcgcag aaatataaac cagaatatgt ctttattcgt aataagcctc
    32401 tggcaatgac cgtcgggatt tggtgttttg cctttaccgc ctttgcctgt ttgacgggga
    32461 tcttcccgaa aatggaagcc ttcactgcag agtggacctt ccagttggcg ctgaatgttg
    32521 caacgccgtt tgtgctggta ggattaggac tgatattccc gctgctggcg cgtaaagcga
    32581 atagtaaata attattgtgg tcggtgacgc tgactctggc agatctgacc cgtttttaca
    32641 taatcgagaa atgcgcgcaa gccgcaggac atatactggc ggtttgggta atagatctgg
    32701 aagcctggac gctctgtgat ccagtcttcc agtacacatt ctaaacgacc ggtatcaagg
    32761 tactccttga tctgttcata gagcagatac ccgataccag cacccatcaa aacggcatcc
    32821 agttcagcat caacatcat
//
LOCUS       DAZPDU010000030        32585 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZPDU010000030 DAZPDU010000000
VERSION     DAZPDU010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32585
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.030"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            219..1592
                     /gene="aroP"
                     /locus_tag="ACHURS_001289"
     CDS             219..1592
                     /gene="aroP"
                     /locus_tag="ACHURS_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFR1237068.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAIVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(1635..2489)
                     /gene="ampE"
                     /locus_tag="ACHURS_001290"
     CDS             complement(1635..2489)
                     /gene="ampE"
                     /locus_tag="ACHURS_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFR1237069.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2486..3037)
                     /gene="ampD"
                     /locus_tag="ACHURS_001291"
     CDS             complement(2486..3037)
                     /gene="ampD"
                     /locus_tag="ACHURS_001291"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFR1237070.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            3125..4018
                     /gene="nadC"
                     /locus_tag="ACHURS_001292"
     CDS             3125..4018
                     /gene="nadC"
                     /locus_tag="ACHURS_001292"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFR1237071.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVSTRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4221..4661
                     /gene="ppdD"
                     /locus_tag="ACHURS_001293"
     CDS             4221..4661
                     /gene="ppdD"
                     /locus_tag="ACHURS_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFR1237072.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            4671..6056
                     /gene="gspE"
                     /locus_tag="ACHURS_001294"
     CDS             4671..6056
                     /gene="gspE"
                     /locus_tag="ACHURS_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFR1237073.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVNQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQKLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRQQQGEPIHIPVNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            6046..7248
                     /gene="hofC"
                     /locus_tag="ACHURS_001295"
     CDS             6046..7248
                     /gene="hofC"
                     /locus_tag="ACHURS_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFR1237074.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGFLLAIANKLLMRRPTWLIVRQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNTGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPTLLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(7283..8326)
                     /gene="guaC"
                     /locus_tag="ACHURS_001296"
     CDS             complement(7283..8326)
                     /gene="guaC"
                     /locus_tag="ACHURS_001296"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFR1237075.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(8482..8526)
                     /locus_tag="ACHURS_001297"
     CDS             complement(8482..8526)
                     /locus_tag="ACHURS_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1237076.1"
                     /translation="MIIKTLKMSARKRN"
     gene            8551..9171
                     /gene="coaE"
                     /locus_tag="ACHURS_001298"
     CDS             8551..9171
                     /gene="coaE"
                     /locus_tag="ACHURS_001298"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFR1237077.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9171..9914
                     /gene="zapD"
                     /locus_tag="ACHURS_001299"
     CDS             9171..9914
                     /gene="zapD"
                     /locus_tag="ACHURS_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFR1237078.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            9924..10121
                     /gene="yacG"
                     /locus_tag="ACHURS_001300"
     CDS             9924..10121
                     /gene="yacG"
                     /locus_tag="ACHURS_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFR1237079.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(10221..10610)
                     /gene="mutT"
                     /locus_tag="ACHURS_001301"
     CDS             complement(10221..10610)
                     /gene="mutT"
                     /locus_tag="ACHURS_001301"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFR1237080.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(10670..13375)
                     /gene="secA"
                     /locus_tag="ACHURS_001302"
     CDS             complement(10670..13375)
                     /gene="secA"
                     /locus_tag="ACHURS_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFR1237081.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(13437..13949)
                     /gene="secM"
                     /locus_tag="ACHURS_001303"
     CDS             complement(13437..13949)
                     /gene="secM"
                     /locus_tag="ACHURS_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFR1237082.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(14180..15097)
                     /gene="lpxC"
                     /locus_tag="ACHURS_001304"
     CDS             complement(14180..15097)
                     /gene="lpxC"
                     /locus_tag="ACHURS_001304"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFR1237083.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(15198..16349)
                     /gene="ftsZ"
                     /locus_tag="ACHURS_001305"
     CDS             complement(15198..16349)
                     /gene="ftsZ"
                     /locus_tag="ACHURS_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFR1237084.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(16410..17672)
                     /gene="ftsA"
                     /locus_tag="ACHURS_001306"
     CDS             complement(16410..17672)
                     /gene="ftsA"
                     /locus_tag="ACHURS_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFR1237085.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(17669..18499)
                     /gene="ftsQ"
                     /locus_tag="ACHURS_001307"
     CDS             complement(17669..18499)
                     /gene="ftsQ"
                     /locus_tag="ACHURS_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFR1237086.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(18501..19421)
                     /gene="ddlB"
                     /locus_tag="ACHURS_001308"
     CDS             complement(18501..19421)
                     /gene="ddlB"
                     /locus_tag="ACHURS_001308"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFR1237087.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(19414..20889)
                     /gene="murC"
                     /locus_tag="ACHURS_001309"
     CDS             complement(19414..20889)
                     /gene="murC"
                     /locus_tag="ACHURS_001309"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFR1237088.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLIGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(20943..22010)
                     /gene="murG"
                     /locus_tag="ACHURS_001310"
     CDS             complement(20943..22010)
                     /gene="murG"
                     /locus_tag="ACHURS_001310"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFR1237089.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(22007..23251)
                     /gene="ftsW"
                     /locus_tag="ACHURS_001311"
     CDS             complement(22007..23251)
                     /gene="ftsW"
                     /locus_tag="ACHURS_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFR1237090.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(23251..24567)
                     /gene="murD"
                     /locus_tag="ACHURS_001312"
     CDS             complement(23251..24567)
                     /gene="murD"
                     /locus_tag="ACHURS_001312"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFR1237091.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIAQAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQLGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(24570..25652)
                     /gene="mraY"
                     /locus_tag="ACHURS_001313"
     CDS             complement(24570..25652)
                     /gene="mraY"
                     /locus_tag="ACHURS_001313"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFR1237092.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(25646..27004)
                     /gene="murF"
                     /locus_tag="ACHURS_001314"
     CDS             complement(25646..27004)
                     /gene="murF"
                     /locus_tag="ACHURS_001314"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFR1237093.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLNAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(27001..28494)
                     /gene="murE"
                     /locus_tag="ACHURS_001315"
     CDS             complement(27001..28494)
                     /gene="murE"
                     /locus_tag="ACHURS_001315"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFR1237094.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKAKDEATDGEIREMHGVPVIYLSQLNERLSALAGR
                     FYHEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENT
                     TGSAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHG
                     DMEHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWL
                     KAIDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKT
                     AARLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDR
                     DKGKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAV
                     TCAVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(28481..30247)
                     /gene="ftsI"
                     /locus_tag="ACHURS_001316"
     CDS             complement(28481..30247)
                     /gene="ftsI"
                     /locus_tag="ACHURS_001316"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFR1237095.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPGSIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(30263..30628)
                     /gene="ftsL"
                     /locus_tag="ACHURS_001317"
     CDS             complement(30263..30628)
                     /gene="ftsL"
                     /locus_tag="ACHURS_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFR1237096.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(30625..31566)
                     /gene="rsmH"
                     /locus_tag="ACHURS_001318"
     CDS             complement(30625..31566)
                     /gene="rsmH"
                     /locus_tag="ACHURS_001318"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFR1237097.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAVATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(31568..32026)
                     /gene="mraZ"
                     /locus_tag="ACHURS_001319"
     CDS             complement(31568..32026)
                     /gene="mraZ"
                     /locus_tag="ACHURS_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFR1237098.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAVGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT         7737 a         9154 c         8039 g         7655 t
ORIGIN      
        1 ctgagcttta caaacggttt ctttttaagc aactcatctt caaccatgca taaagcgggt
       61 gcattcgctg ccgcatacca ttattcttga tctgacggaa gtctttttgt aacaattcaa
      121 acttctttga tgtaaaaaaa ttaatacaac aaacggaatt gcaaacttac acacgcatca
      181 ctgcgtagat caaaaaaaca atcaccgcac gaggtttcat gatggaaggt caacagcacg
      241 gcgagcagct aaagcgcggc cttaaaaacc gccatattca gcttatcgcg ctgggtggcg
      301 cgatagggac agggctattc ctgggtagcg cctccgtaat acagtccgca gggccaggga
      361 ttatcctggg ttacgccatt gctggtttta tcgcctttct gatcatgcgt cagctgggtg
      421 aaatggtggt cgaagaacct gtcgcaggct cctttagcca ctttgcttat aaatactggg
      481 gcagttttgc cggtttcgcc tctggctgga actactgggt actgtacgtt ttagttgcca
      541 tggcagagct gactgccgtg ggtaaataca ttcagttctg gtatccggaa atcccaacct
      601 gggtttctgc cgccgtattc tttgtggtga ttaacgccat caacctgacc aacgttaaag
      661 tgtttggcga gatggagttc tggtttgcca ttatcaaagt tattgcgata gtagcgatga
      721 tcatcttcgg cggctggctg ctgttcagtg gcaacggcgg cccgcaggcg agcgttagca
      781 acctgtggga tcaaggtggt ttcctgccgc acggcttcac cggactggtg atgatgatgg
      841 cgattatcat gttctcgttc ggtggtctgg aactggtggg gatcaccgca gcagaagctg
      901 ataacccgga gcaaagtatc ccgaaagcga ctaaccaggt tatctaccgc atcctgattt
      961 tctatattgg ttcgttagcc gttctgctct cactgatgcc gtggacccgc gttaccgccg
     1021 ataccagtcc gtttgtgctg atcttccacg agttaggcga tacctttgtg gcgaatgcgc
     1081 tgaacatcgt ggtactgact gcggcgctct ccgtgtacaa cagctgcgta tattgcaaca
     1141 gccgtatgct gtttggtctg gcacaacagg gtaatgcgcc aaaagcgctg gcgtctgtcg
     1201 ataaacgcgg cgtaccggtt aacaccattc tggtgtctgc gctggttaca gcattgtgcg
     1261 tattgattaa ctatcttgct ccggaatccg catttggcct gttaatggca ctggtggtat
     1321 ccgcactggt gatcaactgg gcgatgatca gtctggcgca tatgaagttc cgtcgcgcca
     1381 agcaggaaca aggcgtggta actcgcttcc ctgctctgct ttatccgctg ggtaactgga
     1441 tctgcctgct gtttatggcg gcggtactgg tgattatgct gatgacccca ggaatggcga
     1501 tttcggtata cctgatcccg gtatggctga tcgtgttagg tatcggctat ctgtttaaag
     1561 agaaaaccgc caaagccgta aaagcgcatt aatctctcta cgccctcacc catgcagggt
     1621 gagggcaata atctttacac caacgctccg taaatcgtca gtagcgcaat caccaccacg
     1681 accacgaacg aggttttctt cgccattgaa accgctgcct tcggcgtctc caccttatcg
     1741 acatgcggtt cacgcgccag agagaactgc gccagacgcg ttaacacctg atactgcgaa
     1801 gtatggaagt cacccagcga agcaaaccag gccggtaacg ctttctcccc atgaccgatc
     1861 aaggcatata ccacacccgc aagacgaacc ggcacccaat ccagtacatg aagcacggca
     1921 tcaatgccgg actgtaaacg atgatgcggc gtctggtatc gcgccagcca gtattgccat
     1981 gcacgcaaaa acgcataccc catcagcgta acgggtcccc aggttccccc cacaatcaac
     2041 cagaacagcg gtgcaagata aaaacgaaag ttaatccaca gcaatgcatt ttgcagctca
     2101 cgcaaatact cacgttcgtc gcagcctgcc gggacgccgt gaatcatggt gagttcgcca
     2161 gccatcgtgg cacgggcatg gctatcatta cgtgaagcag ctgtcagata agcatgataa
     2221 tgaagacgaa ctttacctgc gccaatacac agcaaaccaa tcagcagcca caccagcagc
     2281 gtgggaacgt tgaacaatac tccctgcaat gcgcgtaaca gtaaaaaagt cacgcccatc
     2341 gcaataatgg tcatgcctaa cgtgcgcccg agagaaaaat gtttcacccg ccgaaagaac
     2401 gcttcaagac gatgatcaag ctgccagtgc tcgcccaact taaacaggcg ctcgaaaatt
     2461 aacaccagta aggttgtaaa tagcgtcatg ttgtctcctt gctgactagc gcacgaaacc
     2521 gtgcccaatc aaatgcagga ccgggatcgg ttttccgatc cggcgcaata tcacaatggc
     2581 ccgtcatgtt attagcgata tcgggatagc gatctatcag tgcccgcgta accgccgcaa
     2641 gctgttgata ctgcgcatcg gtatacgcca gagtatcggt gccttcaagc tcaatcccaa
     2701 tagaaaaatc attgcagcgt tcacgcccct gatactgaga gactcccgca tgccaagcac
     2761 gtttatcgaa aggaacatac tggactattt caccatcacg gcgaatcaaa cagtgagcgg
     2821 agacgcgcaa atgggcgatc tcagcaaaga aaggatgtgc ctgcggatca atagttccag
     2881 tgaataatgc gtcgatccac ggaccgccaa actcgcctgg cggcaggcta atattgtgca
     2941 ccaccagcag ggtgggtgtt tcgtcatccg ggcggcaatc gtaatgtggt gagggaacgc
     3001 ggcgcgcgcc aaccagccac ccctgttcta acaacatgca ggatctcctt atatgtggtg
     3061 ctaatacccg gttcagagta gcatgtttct accttatgat tcgttagcta tctggagttt
     3121 taacatgccg cctcgccgct ataaccctga cacccgacgt gacgagctgc tggaacgcat
     3181 taatctcgat atccccggcg cggtggccca ggcgctgcgg gaagatttag gcggaacagt
     3241 cgatgccaac aatgacatta cggcaaaact tttaccggaa aactctcgct ctcatgccac
     3301 ggtgagcacc cgcgagaatg gcgtattttg cggcaaacgc tgggttgaag aggtgtttat
     3361 tcaactggca ggcgacgatg tcaccataat ctggcatgtg gatgacggcg atgtcatcaa
     3421 cgccaatcaa cccttgttcg aacttgaagg cccatcccgc gtgctgttaa cgggcgaacg
     3481 cactgcgctt aattttgtgc aaaccctttc aggagttgcc agtaaggtac gccactatgt
     3541 cgaattactg gaaggcacca acacgcagtt gttggatacg cgcaaaacct tacctggcct
     3601 gcgttcagct ctgaaatacg cggtactttg cggcggcgga gcgaatcacc gtctggggct
     3661 ttctgatgcc ttcctgatca aagaaaatca tattattgcc tccggctcag tgcgccaggc
     3721 cgtcgaaaaa gcgtcctggc tgcacccgga tgcgccagta gaagtcgaag tagagaatct
     3781 ggaagaactt gatgaagccc tgaaagcagg agccgatatc atcatgctgg ataacttcga
     3841 aacagaacag atgcgcgaag ccgtcaaacg caccaacggc aaggcgctac tggaagtgtc
     3901 tggcaacgtc actgacaaaa cactgcgcga atttgccgaa acgggcgtgg actttatctc
     3961 cgtcggtgcg ctaactaaac acgtacaagc actcgacctt tcaatgcgtt ttcgctaatt
     4021 gctgacagat ttatccgctc catcgcgggc ggataatctc cccttcccca actttcttcg
     4081 taacgcctcg caaatttctt gtcttttgct gcaacgaaat aaaaaacctc ggaagctgct
     4141 ttcccgtttc gttttttgcc cctcgcttgt gctgatggcg tttgccaaag tagcgccaac
     4201 caaatcaagg agcgaaacag atggacaagc aacgcggttt tacacttatc gaactgatgg
     4261 tggttattgg catcattgcc attttaagcg ccattggtat tcccgcttat caaaactacc
     4321 tgcgcaaagc tgcactcacc gacatgctac aaacctttgt gccttaccgt acagccgtag
     4381 agttgtgcgc gctggaacat ggtggattag atacctgcga cggtggcagc aatggcattc
     4441 cctcgcctac caccacccgc tatgtttcag ccatgagtgt ggcaaaaggc gtggtgtcgc
     4501 tgaccgggca agaaagtctc aatggactaa gcgtcgtcat gacaccgggt tgggataacg
     4561 caaacggcgt caccggctgg gcgcgcaact gcaatattca aagtgacagc gcattgcagc
     4621 aagcctgcga agatgtcttc cgctttgatg acgccaacta aggagcggca atgaatattc
     4681 cacagctcac cgccctgtgt ctgcgttatc agggagtctt gctggatgcc agcgaagagg
     4741 tggttcatgt tgcggtagtc gatgcacctt cgcatgagtt gctggacgca ttgcatttcg
     4801 ctaccaccaa acgtattgag atcacctgct ggacgcgcca acaaatggaa ggccacgcca
     4861 gtcgcacaca acagacattg cccgtagctg ttcaggagaa gcatcagccc aaagcagagt
     4921 tgctgactcg aacgttacaa tctgcgctgg aacaacgtgc gtctgatatt catatcgaac
     4981 cagcggacaa tgcctaccgc atccgcttgc gtatcgacgg cgtattgcat cctttaccgg
     5041 atgtttcacc ggatgccgga gtcgcattaa ccgccagatt aaaagtgctg ggaaacctgg
     5101 atattgcgga acatcgcctg ccgcaggacg ggcaattcac tgtcgaactg gcaggaaacg
     5161 ccgtctcatt tcgtattgcg accttaccat gtcggggtgg tgaaaaggtg gtattaaggt
     5221 tgttacagca ggtgaaccag gcactggatg ttaacacgct gggaatgcag ccgttacaac
     5281 tggcggactt tgctcatgcc ttgcaacaac cacagggact ggtgctggta actggcccta
     5341 caggcagcgg caaaacggtc acgctttata gtgccctgca aaagctgaat accgctgaca
     5401 ttaatatttg tagcgtcgaa gatccggttg agatccccat agccggacta aaccagacgc
     5461 aaatccatcc gcgtgccggg ctcacctttc agggcgtttt gcgtgcgtta ttgcgccagg
     5521 atcctgacgt catcatgatc ggagagatcc gcgatggcga aacggcagaa attgccatta
     5581 aagccgcgca aaccggtcac ctggtgttgt ctaccctaca cactaattcc acctgcgaaa
     5641 cgctggtacg tttacagcaa atgggagtcg cccgctggat gctctcatca gcgcttacgc
     5701 tggtaatagc ccagcgtctg gtacgtaaac tttgcccaca ttgtcgccag cagcaagggg
     5761 agcccatcca tattccagtc aatgtatggc cgtcgccgct gccccactgg caggcacccg
     5821 gttgtgtaca ttgctaccac ggtttttatg gtcgtacggc cttatttgaa gttctgccca
     5881 taacgccggt cattcgtcag cttatttccg ctaataccga cgttgaatcg ctggaaacgc
     5941 acgcacgaca ggcgggtatg cgtacgcttt ttgaaaacgg ctgcctggcc gtggagcaag
     6001 gcttaaccac ctttgaagag ttaatccgcg tactggggat gccgcatggc gagtaagcaa
     6061 ctctggcgct ggcatggcat aaccggcgac ggcaatgcgc aagatgggat gctatgggca
     6121 gagagccgtg ctttgctgct catggcacta cagcaacaga tggttacccc acttagcctg
     6181 aagcgaatcg ccatcaattc tgcgcagtgg cgaggagata aaagcgcgga agtcattcat
     6241 caactggcga cgctactcaa agccgggtta acgctttctg aagggctggc actgctggcg
     6301 gaacagcatc ccagtaagca atggcaagcg ttgctgcaat cgctggcgca cgatctcgaa
     6361 cagggcattg ctttttccaa tgccttatta ccctggtcag aggtatttcc gccactctat
     6421 caggcgatga tccgcacggg tgaactgacc ggtaagctgg atgaatgctg ctttgaactg
     6481 gcgcgtcagc aaaaagccca gcgtcagttg accgacaaag tgaaatcagc gttacgttat
     6541 cccatcatca ttttagcgat ggcaatcatg gtggttgtgg caatgctgca ttttgttctg
     6601 ccggagtttg ccgctatcta taagaccttc aacaccccac taccggcact aacgcagggg
     6661 atcatgacgc tggcagactt tagtggcgaa tggagctggc tgctggtgtt gttcggcttt
     6721 ctgctggcga tagccaataa gttgctgatg cgccgaccga cctggcttat agtgcggcag
     6781 aaattgctgt tacgcatccc gattatgggt tcactgatgc ggggacaaaa actcacgcag
     6841 atctttacga ttctggcgct gacacaaagt gcaggcatta cttttttgca gggcgtagag
     6901 agcgtcagag aaacaatgcg ctgcccgtac tgggtgcaac ttctgacaca aatccagcac
     6961 gatatcagta acggtcatcc catctggctg gcgctaaaaa ataccgggga gtttagcccg
     7021 ctctgtttgc aattagtgag aacaggagag gcatccggct cgctggacct catgttagac
     7081 aacctcgccc atcatcatcg ggataacaca atggcgctgg cggataacct cgcagcctta
     7141 ctggaaccga cgttgctgat cataacggga ggaattatcg gtacgctggt ggtggcgatg
     7201 tatctgccaa ttttccattt aggcgatgcg atgagtggga tgggataacg ctggcgtgtt
     7261 gctccacacc agcgttggaa gattacaggt tgttgaagat gcggttttct tgttcctgca
     7321 cacggataaa tgtggtgcgc ttggtcagtt ctttcaggcg tgaagcccca acgtatgtac
     7381 aagctgaacg caggccgccc aaaatatcac gcgcggtatt ttcaaccggg cctcgcagcg
     7441 gcagcttaac ggttttacct tctgctgcgc gatattccgc aacgccgcca acgtgacgtt
     7501 tcatcgcaga ctcggagctc atgccgtaga acagcataaa tttctcgccg ttctcctcaa
     7561 cgatgcgacc gccgctctct tcgtggcccg ccagcatgcc gccaagcatg acgaaatcgg
     7621 caccgccgcc aaaggctttt gcgacatcgc ccggcgtggt gcagccaccg tcgctgacga
     7681 tcattccgcc cagaccgtgc gccgcatcgg cacattcgat taccgcagaa agctgcggat
     7741 aaccgacgcc tgttttgacg cgagttgtac aaacagaacc tgggccaatg ccaactttaa
     7801 cgatatcagc accggagagg ataagctcct cacacatttc accagtcact acgttgccag
     7861 cacaaatggt tttggtcggc cacgcttcac gcgctttcgc aacgaactgc acgaagtgtt
     7921 cggaataacc attcgccaca tcaatacaaa cgaagtttaa tgccgggttc aggtcgagaa
     7981 tctgtttagt tttttcgaaa tccgcatcag acgtaccggt agaaaccatc acatgtttca
     8041 gcacatcagc ggaagaattg ttgataaacg cttgccactc ttcgacagaa tagtgtttat
     8101 gcacagcagt caaaatatca aaagaagcca gcgcagaggc catagaaaat gtacctacgg
     8161 tgtccatatt tgcggcgata atcggcacgc cggaccagct ctgacctgaa tgtttgaagg
     8221 tgaattgacg ttccagttca acatcggaac ggcttttaag agtggagcgt ttagggcgga
     8281 tgagaacgtc tttaaaacct aacttcagat cttcttcaat acgcatgtgc ggattcctgg
     8341 ggttaatggc tataagggta agagcgcaac tccagtgacg ctatcatacg cactaatcaa
     8401 tgcggcgcaa gactgcgaaa ttgtcttttt ttacgctaca atcccataaa tttacctggt
     8461 aaatagtgag cgaacgtttc atcaatttcg ctttcgtgca ctcattttta acgttttgat
     8521 aatcatgatg aattcccggg aatataaatt atgaggtata tagttgcctt aacgggaggc
     8581 attggcagtg gcaagagtac cgttgccaat gcgtttgctg atctcggaat taacgtcatt
     8641 gatgccgata ttattgcgcg tcaggtggtt gaaccaggtg cacctgcgct acatgccatt
     8701 gctgatcact ttggcgctaa catgattgct gctgatggaa cattgcagcg ccgggccttg
     8761 cgcgagcgga tcttcgccaa cccggaagag aaaaactggc ttaacgccct gctgcatccg
     8821 ctgattcagc aagagacgca acaccagatc cagcaagcta cttcccccta tgtactgtgg
     8881 gttgtgccat tgctggtaga aaactcactg tataaaaaag cgaatcgagt gctggtggtg
     8941 gatgtcagcc cagaaacgca acttaagcgc accatgcagc gcgatgatgt aactcgcgag
     9001 catgtcgaac aaatccttgc tgctcaggca acgcgcgaag cccgccttgc cgtggcagat
     9061 gacgtcattg ataataacgg cgcaccggat gctatcgcat cggatgttgc ccgcctgcac
     9121 gcacactatt tgcagcttgc gtcgcagttt gtctcacagg aaaaaccgta atgcagaccc
     9181 aggtcctttt tgaacatcca ctaaatgaaa aaatgcgtac atggctgcgc attgagtttt
     9241 tgattcagca actcaccgtt aatttaccca tcgttgacca cgctggcgcg ctgcatttct
     9301 tccgtaatgt cagtgaatta ctggatgttt tcgagcgcgg cgaagtccgc actgagctgt
     9361 tgaaagaact tgaccggcag caacgtaaac tccagacctg gattggcgtg cctggcgtgg
     9421 accagagccg tattgaagca ttaattcagc agttaaaagc ggcggggagc gtattaattt
     9481 ccgcgccgcg tatcgggcaa tttctgcgtg aagatcgttt gattgctctg gtgcgtcagc
     9541 gactgagcat cccaggcggc tgttgcagct ttgatttacc tacattgcac atttggctgc
     9601 atctacccca ggcgcagcgc gacagccagg tagaaacctg gattgccagc ctgaacccgc
     9661 tcacccaggc acttaccatg gtgctggatt taattcgcca gtcggccccc ttccgtaaac
     9721 aaaccagtct gaatggtttt tatcaggata acggtggcga tgccgacttg ctgcgcctga
     9781 atctgtcgct cgattcacag ctttatccgc aaatttccgg acataagagc cgttttgcca
     9841 ttcgttttat gccgctggac actgaaaacg gacaggtacc ggaacgtctg gatttcgaac
     9901 tggcctgttg ctaaggagta aaaatgtcag aaactattac ggtgaattgc ccaacctgcg
     9961 ggaaaacggt ggtgtggggt gaaatcagcc cgtttcggcc attttgctcc aaacgttgtc
    10021 agctgatcga cctcggagaa tgggctgctg aagaaaaacg aatccccagc agtggcgatc
    10081 tttccgaaag cgatgactgg agcgaagagc caaagcagtg acatttgtct gatgccgcac
    10141 gtaggcctga taagacgcgg acagcgtcgc atcaggcatc ttgtgcaaat gtcggatgcg
    10201 gcgtgaacgc cttatccgac ctacagacgc ttaagcttcg caattaccgg ttcattggct
    10261 ggcggaaaat catcggcatt aagaccgact agcgacatcc actcaccggg ttgcccttct
    10321 ttaccccacg gctccccttc ccagctttcg accagccaga accacagagt tatatgcctg
    10381 tccgggaatt catattccag tttttcaaat agcgaaaaat gttgtggggt aatcccgact
    10441 tcttcctgaa gttcacgcac taccgcctgt tccggcgttt cgcccatttc aattttaccg
    10501 ccgggaaact ccagtttatt cgccatgtgc gcatctgctg cgcgacgcgt tataaagatt
    10561 tcattgttct cgttgcgaat aatacctacc gcaatttgca gctttttcat tgtcttaaac
    10621 ctataaaaaa ggcgcagaat cctgcgcctt ttacttcaac tgttagcttt tattgcaggc
    10681 gaccatggca ctgcttgtat tttttaccag aaccgcacgg gcaaggatcg ttacgtccta
    10741 ctttgcgctc accggtttgc gccgccagtg cagctgcggc tgcagagtcg tcatcctgat
    10801 ggctaagctg ctgcatttgc gctaaacgct cggcttccat acgacgctgt tgttccagct
    10861 cctcaacctc ttcaggcata cgtacctgga ctttgctcag cgtgctgata acttcatatt
    10921 ttaacgactc cagcatcgct gcaaacatgg agaacgattc acgtttgtat tcctgcttcg
    10981 gatctttctg tgcatagcca cgcaggtgga taccctgacg cagatagtcc atcgccgcta
    11041 ggtgctcttt ccacagagag tcgagagttt gcagcatgac gcctttttcg aagtgacgca
    11101 tcatctcagc accaaccact tcttctttac gctgatacac ttcgatggac tgcgccagaa
    11161 ttcgctcacg cagcgtctct tcatgcagtt ctggttcttt atccagccac tcggcaattg
    11221 gcaaatcgag gtcgaaatcg ttcttcagac gttcctgcag ccccggaata tcccacattt
    11281 cttccagtga ctgtggcgga atgtaggcat caatggtcgc tttgaacaca tcttcacgaa
    11341 tgctgttgat ggtttcgctc acatcgctga catccagcag ttcgttacgc tgggagtaaa
    11401 tggcgcgacg ctgatcgtta gccacgtcat catattccag cagttgctta cgaatgtcga
    11461 agttacggct ttcaacttta cgctgggcgt tggcaatcgc tttagtcacc cacgggtgtt
    11521 caatggcttc gcctggcttc atacccagtt tacgcatcat gccggatact cggtcggaag
    11581 caaaaatacg catcagcgca tcttccatcg acaggtagaa acgggaagaa ccagcatccc
    11641 cctgacgacc agaacgaccg cgcaactggt tatcgatacg acgggattcg tgacgctcgg
    11701 taccgatgat atgcaggcca cctgcttcca gtaccgcatc gtgacgtacc tgccagtcgg
    11761 ctttaatttt ttcaatttgc tctgcggtcg gattttccag cgcggcaact tctgcctgcc
    11821 agctaccacc gagcacaata tctgtaccac gacccgccat attggtcgcg atagtcaccg
    11881 cagccggata acctgcctga gcaacaatcg ccgcttcgtt ggcgtggaat ttggcgttca
    11941 ggacgttgtg cttaataccg gctttggtca gttcgtttga caccagctcc gatttttcga
    12001 tggagatagt acccaccagc accggctggc ctttcgcagt acgttctttg atatcttcaa
    12061 tgatcgcctg aattttttcc gcttcagtca tgtagaccag gtccggcaga tctttacgaa
    12121 tcattggacg gttggtcgga acaacgacgg tatccagctt gtagatggag ctaaattcga
    12181 aagcttcggt atcagcagta ccggtcatcc ccgccagttt ttcatacaga cggaagtagt
    12241 tctggaaggt gatcgaagcc agcgtttggt tttcgttctg gatctgcaca ccttctttcg
    12301 cttccacagc ctggtgcaga ccatcggacc agcgacggcc ctgcatggta cgaccggtgt
    12361 gttcgtcaac gatgataact tcaccatctt taacgatgta gtcgacgtcg cgggtaaaca
    12421 gcgcatgagc gcgcagcgcc gccgttacgt ggtgcatcag catgatgttg gccggagagt
    12481 acagagactc cccttcatcc atgatgccct ctttcaccag cagttcttca atcagcacca
    12541 gaccacgttc ggtcaggttc acctggcgag atttttcgtc caccgagaag tggccttcgc
    12601 cctggaaggt ttcggagtct tctttttcct gacggatcag gtgcggaata attttattca
    12661 cgcgtttata catttccgag ctgtcttctg ccgggccgga aatgatcagc ggtgtacgcg
    12721 cttcatcgat caggatggag tccacttcgt ccaccagcgc atagtgcagt ttacgctgta
    12781 cacgttcttc agggctgaac gccatattgt cgcgcaggta gtcaaagccg tattcgttgt
    12841 tcgtaccgta agtgatgtca gctgcgtaag cttcgcgctt tgccggtgct ggcatgcccg
    12901 gcaggttgat accgacagtc aggccaagga attcaaacag cggacggttg ttttcggcgt
    12961 cacgttgcgc caggtagtcg ttgacggtaa ctacgtgcac gcctttaccg gttagtgcgt
    13021 tcaggtaagc aggcagcgtt gcggtcaggg ttttaccttc accggtacgc atttcggcga
    13081 tgcagcgttc gttaagaacc ataccgccga gtaactgaac gtcgaagtga cgcataccaa
    13141 agacgcgctt acttgcctca cgtaccacgg cgaaagcttc cgggatcaga ttttccagca
    13201 cttcgccttt ttccagacgt gcacgaaact ctgcggtttt ccctttcagt tcttcgtcgg
    13261 agagtttttc catctccggt tccatggcat tgatgatgtt gaccactttg cgcatccggc
    13321 gcagggtgcg atcgttacga ctaccgaaaa ctttagttaa caatttgatt agcataataa
    13381 aatctcaaac gccccgcgtt gcggagttaa taaaatgaag taaaggttta ttgttgttag
    13441 gtgaggcgtt gagggccagc acggatgcct tgcgcctggc ttatccagac gggcgtgctg
    13501 aattttgctt gtggggtaaa atgcgcataa tcaatgcgat aacccttttc agacggcgtg
    13561 ccttcctggg tcagcagcgc gctgagcgta tccagtaatg caagatgttg cgcctgaaga
    13621 ggcaaagatt cttcagcaac gggcagtgtt tgcggtgcca ttgcgaaaga aagatgacgg
    13681 attaccgtgc gaatggcatg ttgatgccag taatcaacgg aatagttcga attcgggcgg
    13741 cgtgtgttcg cttccagcaa ggccaattga ccaaagttaa ctttggctga aggctcgtgg
    13801 ttgcgggttg tcgcttttgc gggcgcgttt ggttcggcgg cgttgctgag cgcaggcaaa
    13861 cctaaactcg ccgcaaccat ccctaataag agatgcggcc agaagtagcg tttaccaaac
    13921 tgtcgccagc gcgtcagtat tccactcacg ttattgccat cccgatcgta ttcaaacgta
    13981 tttagcgcac aaatcttatc attaaagcct gcagtccaca gcacgaatga agataagttg
    14041 tgcggaaaag tgcttaagaa aacagcgttc gcaccgtttt ttatagctct tataaaattc
    14101 aaaaaaaaat gactggttca cctggccgga gagagtgcca gatttgccag tcgaatttta
    14161 tacgacagta taaatgtcgt tatgccagta cagctgaagg cgctttgaag gccaacggca
    14221 gttctgcgtc gtcctggaag gtcacatatt cccaggcttc ctgtttcgcc aggacagcct
    14281 gcagcagttt gttattcagt gcatgaccgg atttataagc ggtaaatgca ccaataatat
    14341 tgtgaccaca catgaacaag tcaccgatcg catcgagcat tttgtgacgc acaaattcgt
    14401 cttcaaaacg caggccgtct tcgttcagta cgcgataatc gtcaacaacg atggcacaat
    14461 cgaagctgcc gcccaggcac aaaccacggg actgcagata ttcgatatca cgcatgaaac
    14521 cgaacgtacg cgcacggctg atctggcgca taaacgcatc agcggagaag ttcatcgcat
    14581 agcgctggtt gctggaatca atagccggat ggttaaaatc gatggtgaaa tccagcgaaa
    14641 aaccattgta cggcttaaat tcagcccact tatcgccatc ttcgacacga acagtctctt
    14701 tgatgcgaac aaattttttg gcgcagttca actcgtcgat accggcgtca agcagcaggt
    14761 atacaaacgg agcggcgctg ccgtccatga tcgggatttc cggcgcgtta acttcgataa
    14821 caatgttatc gatgcccagg cccgcgagag cagcattgag gtgctctacg gttgaaatcc
    14881 gtacatcatg ctcgttgacc agacacgtac agagcatggt atcacgcaca gatttggcat
    14941 cggccgggaa atctaccggt ggattcaagt cggtgcgacg atagatgacc ccggtgttgg
    15001 ccggcgcagg gcgtaacgtc agagtgactt tcttgccggt atgtaaaccg acacccgtcg
    15061 cctgaacgat acgtttaagt gtcctttgtt tgatcatcgt attatctcgc caaattacct
    15121 atccaaccga agtgtactat acatccggcg ggccagttta gcacaaagag cctcgaaacc
    15181 caaattccag tcaattctta atcagcttgc ttacgcagga atgctgggat atccagataa
    15241 tccggctctt tcgcagtttg cggcgcattg tcattcacga ctttagcaac cggcttctgc
    15301 tcctgggtca gcggagccat cccatgctgc tggtagcgat ccatcactgg ctgctgaacc
    15361 tgcttattgg tcaccagagt gatttcagga cgtttgtcca tgccgatacc tgtcgcaaca
    15421 acggttacgc gcagctcgtc attcatatcc gggtcaagag aagtaccgat aaccacagtc
    15481 gcgttgtcgg aagcaaatgc acggatggtg ttacctaccg tttcgaactc atccagacgc
    15541 aggtcgaagc ccgccgtgat gttaaccagc acgccgcgcg cgccagacag gtcgatatct
    15601 tccagcagcg gagaagagat agccatttca gcagcttctt ccgcacggtc ttcaccgctc
    15661 gccacgccag aacccatcat tgcgtagccc atctcagaca ttacggtgcg tacgtctgca
    15721 aagtccacgt tcatcaaacc cggacgagta atcagttcag cgataccttg cacagcgcct
    15781 ttcagtacat cgttcgctgc gccaaacgca tccagcaggg agataccgcg gcccagaact
    15841 ttcagcagtt tgtcgttcgg gatagtgatc agagagtcca catgcttgga cagttcagtg
    15901 atcccctgct ccgcgaatgc catacgcttc ttgccttcaa agttgaaagg cttagtgacg
    15961 acagcaacgg tcaggatacc caaatctttt gccacttcag cgacgactgg tgctgcacct
    16021 gtaccggtac caccacccat acccgcagca ataaagacca tgtctgcacc ttccagcgcc
    16081 gcacgcaatg catcgcgatc ctcatcagcc gcattgcggc caacttctgg attagcgcca
    16141 gcgcccagtc ctttggtgat accgctaccg atttgaatcg tctgtccaac cgctgtttta
    16201 cgcagcgctt gtgcatcggt atttaccgcg aagaattcaa caccttcaat acgctcgcgc
    16261 accatgtgtt caacagcatt accgccgccg ccgccgacgc cgatgacttt aatcaccgcg
    16321 tcattggtaa gttccattgg ttcaaacata gtttctctcc gatttgtgcc tgtcgcctga
    16381 ggccgtaatc atcgtcggcc tcataaaaat taaaactctt ttcgcagcca actattgagt
    16441 cgcttgatcc acgagccaac tgatgctgta acacgttttt ctacttcagc ttcaccgtta
    16501 agatgtgact ctttcccata gtgaagcaat cccaccgccg tcgaataata cggctcctga
    16561 gcataatccg ttaaaccggt aatgttcagc ggcgcgccga tacgcacttg cgtatgaaac
    16621 acgcgctgag cacaggctgc aagaccttcg atctgcgctg cgccaccggt taatacaatg
    16681 cctgccgcca ggtgatgttt aaccccttgt tggcgaagct tttcctgcaa ctgcaatatc
    16741 tcttcgttga ccaggttgag cagctcggta tagcgcggct cgatcacctc tgccagggtc
    16801 tgacgttgca gactccgtgg cggacgaccg cctacgctcg gcacttccac gctctcatct
    16861 tttccaacga tggaacccag cgcacaaccg tggcgaactt taatcgcttc ggcgtcgctt
    16921 ggcggcgtgc caaaggcgta agcgatatca ctggtcacga cattgccagc ataaggaatt
    16981 accttagtgt ggcgcaatgc cccaccggta taaacggcga tatccattgt accaccaccg
    17041 atatcgacga cgcagacacc cagttcacgt tcatcttccg tcaataccga ataacttgat
    17101 gccagtccgg caaatatcag ttggtcaact ttcagcccac aacgttcaac cgctttgacg
    17161 atgtttttcg ccatatcgtt gtgacatgtg atcaggtgca cttttgcctg catccgcacg
    17221 cccgaaagtc ctaccggatt cttgatccct tcctgatagt caatcgcata ctcttgcggg
    17281 atcacatgca gcacacgatg ttcatcgcgc acacgcaccg atttcgcggt atggacgacg
    17341 ttttccacat cttcttgcgt cacttcttct tcagaaatag gcaccatacc aatttcattc
    17401 tggcagctga tgtgcttacc agaaagcgcc agatataccg aagagatctg acaatctgcc
    17461 atcaattctg cctggtcaat ggcgcgttgt acgcacttga ccacggattc gaggtcgttc
    17521 accccgcctt tatccatacc acgtgacggg cagctgccca cgccaatgat attgaccata
    17581 ccgtcgggca gaacttcccc tactaaagcg gcaaccttcg cggtaccaat ctccagtcct
    17641 actaccagtt ttctgtccgt cgccttgatc attgttgttc tgcctgtgcc tgattttgtt
    17701 gctgagtaga ttcctctggc ggcaagggcg cccagcctac tgccgctcca gagtcataac
    17761 gcaaatcaac gtagctaatc cgtttgccat cggtttgcgc ctgctgctgt aaaaccggat
    17821 aaagttctac aaagcgagcc aaacgtttca tcgtatcgcc ccggccaaga ttgagcttaa
    17881 tatcgttatt cagcgtcaac tgccaggaac gccgcgcggt catcgccgct tccttcagag
    17941 taaatctgtc ctttgccagc atctgcccca tttcgcgata gccctgcaac acttcattgg
    18001 cgctgccttc cgggccatac agcattggaa gcacctgctt gctggtgcga tctggcggca
    18061 cgctgaaggt gtttccttcc gcgtctacca tatgttgatc attccaccgc gcaatcggca
    18121 catattcaac cagatgaatc ttcaattcat caggccactg ctttctgacg ctcacctgct
    18181 taatccacgg caggcgttgt tctatttgcg tctggatgat gttgacatcc tgggtcataa
    18241 aggtacccgg ctcacccaat gccaggatcg actgccggat atcgtcatta cgcgtgtaat
    18301 ggcgttcacc ggtcaacacc agctttgaga gcggcaggcg ttgcgcatct tccatccagc
    18361 ccaacacgac ccagccgctc accaacactg tcgttaaaac ggtcagcagg aaaaggatcc
    18421 ccgccagacg cgttccatta ttgcggcgag aagaaacctc ttcttcgcta tttcgcgtgt
    18481 tcagagcagc ctgcgacata ttagtccgcc agttccagaa ttcgtactac caactgcgag
    18541 aagctcatac ctgcctgacg tgccgccatc ggcaccaggc tgtggctggt catacccggt
    18601 gaggtattgg cttccagcag ataaaactgt ccatcgctgt ccagcataac gtcaatacgt
    18661 ccccatcctt tgcaacctaa cgtcgtccat gctttcagca ctaatgcctg caaattggcc
    18721 tcttgtgacg cttccagacc tgcggggcag aaatactgtg tctcatcaga gagatacttc
    18781 gcctcataat catagaaggt tccggacggt tgaatacgta ttgacggtaa aatttcttca
    18841 ccgagtatcg caaccgtgaa ctccggccca cttagccatt tttcaatcaa tacttcttca
    18901 tcgtgctgaa atgccaatct taatgcatct tgtagagcat tttctgctac tacttttgac
    18961 attcccacac tggaaccttc gcggctcggc ttaacgataa ccggcaaacc cagagcagaa
    19021 atttctgcta actgcttatc gctcaggcct ttttcaaact ctgcgcgggt taacgctacc
    19081 cacggcgcga ccggtaaacc ggcaccttgc cacagcagtt tgctgcgtag tttatccatt
    19141 gaaagcgcag atgccatcac tccgctgccg gtataaggca agcccatcag ctcaagcatg
    19201 ccctgcagcg taccatcttc accgccgcga ccgtgtagcg cgataaacac tttctgaaaa
    19261 cccatcgact tcagttgcgt cacgtcgact tctttcgggt cgacaggata cgcatcaata
    19321 ccgccttcac gcagcccggc taacaccgct gcgccagaat tcagagaaac ttcccgctca
    19381 gcggaagtcc cgcccaacag gaccgcaatt ttatcagtca tgttgttctt cctccggagt
    19441 ttgcggcttc agtttgattt cagctaaaga acgggcaatt tttccgatat taccagcccc
    19501 ctgaacgaga atcaggtcgt taccgattaa taccggtgcc agcatctcgg ctacctgcgc
    19561 agggtccggc accagaatgg gatcaatttt cccacgtcca cgaattgtgc gacacagcga
    19621 acggctgtcc gctcccggaa ttggcgcttc gccagccgga tacacttcca gcatcaacag
    19681 ggtatcaacc tgcgtcagca cattggcgaa atcatcatac aggtcgcgcg tacgggtaaa
    19741 acggtgcggc tgaaacagca ttactaggtt tttatccggc cagcctgcgc gcgccgcttt
    19801 aatggtggcg tcaacttccg tcgggtggtg gccgtagtca tcgaccagca ttgccgtacc
    19861 gcttttacca ttcactggct ccagcgggaa ttcaccgagg aaatcaaaac ggcgaccagt
    19921 cccctggaag ctctccagcg cacgcaaaat agcctcgtcg tcaattccct cttccgtagc
    19981 aaccgcaacc gcagctgcgg cgttcagcgc gttatgacgg cctggcgcat tcagggtgac
    20041 gcgcatcggc tctttgtcct ggcgcagcag cgtaaagtgc ccctgcgggc caatctgctg
    20101 ataatcttct acacgcacgt cggcatcttc gctgaagccg taagtcgtgg tctgacgtcc
    20161 cacacgcggt aacaattcgc ggatcaccgg atcatcaaca cacatcaccg cacgaccgta
    20221 aaacggcagg ttgtgcagaa aattaataaa agtctgtttt aaattctcaa agtcgccctg
    20281 gtaggtatcc atgtggtcgg cttcgatatt ggtgacaatc gccaccatcg gttgcagatg
    20341 caggaacgat gcatcactct catctgcttc ggcaatcagg taccgaccat gccccaaacg
    20401 cgcatgaacc cccgccgctt ttaccagccc gccgttaacg aaggttgggt cgagccccgc
    20461 ttctgcgtag atgctggaaa ccatcgcggt ggttgtcgtt ttgccgtgcg ttcctgcaat
    20521 ggcgatgcca tgacgaaaac gcattaactc agccagcatt tcggcacgac ggatcaccgg
    20581 aatacgcgct tcatgagctg cgacaatttc cgggttatcg gcagaaatgg cgctggaaac
    20641 aacgaccacg ctggcatcac gtacgttttc cgggcgatgg ttgaaataaa tcgtcgctcc
    20701 cagattcatt aactgctgcg tgaccggatt tggcgctaaa tcggaaccac tgatctgata
    20761 accttcattg gccagaactt cggcaatacc gcccataccg gcaccaccga tgccgacaaa
    20821 atgtatgtgc cgaacgcgac gcatttcggg cacgatggaa cgcagttttg ccaattgttg
    20881 tgtattcatt ctttacgcca ttaacttctt aaattcatac gatgcaaaag gcatcgctac
    20941 aattacgccc gggcagcccg gctcacttca tttgccactc gctcggtggc atccggaatg
    21001 gatgcagcgc gggcgcgttc tgccatggtt aataaggttt ctcgcgacca cccggccagg
    21061 gtgttggcga cagcatccac gctaagctgt ggctgctcga taattttggc tgcgcccgct
    21121 ttttccagcg gtagcgcatt ccagtattgc tggcggtctt tatgttgaaa cggcacaaac
    21181 aacgccggta gtcctgccgc ggcgatttca ctcaccgtta aagcaccgga gcggcaaacg
    21241 acgacatccg cccacgcata cgccgccgcc atatcatcaa taaattccgt cactttatgc
    21301 tgcggttgcc ccgcttcggc atacgcctgt tcaacggatt gttgcgaacc tttaccgctc
    21361 tgatgccaga tagtgactga atcacccagt ttcgcagcaa cctgcggcat tgtctggtta
    21421 agaatgcgtg cgccctggga accacccact accagcacac gaaccggacc ttcacgtcca
    21481 gccaaacgtt gctgcggcaa cggcagcgcc agcacatcgg tacgcaccgg gttacccacc
    21541 acttccgcat tagggaaagc gcctggaaac gcctgcatca ctttggtggc aatcttcgcc
    21601 agccatttat tggttaagcc cgcaataccg ttttgttcat gaagtacaac cggaatgcct
    21661 aacgaccacg cggccagacc acctggacct gatacatagc cgcccatacc gagtaccacg
    21721 tcaggtttgt acgctttcat aatcgcccgc gcctgacgcc aggcgttgaa gatacgcagc
    21781 ggcgcagcta tcagtgcttt tatacctttt ccacgcagac cagagatacg aatgaaatca
    21841 atttcgatgc catgttttgg cactaagtcc gcttccatac ggtcggcagt ccccagccag
    21901 cgaacttgcc aaccctgagc cattagatgg tgcgcaaccg ccagtcccgg gaatacatgt
    21961 ccaccggttc cgcctgccat caccattaat cgctttcctt gaccactcat cgtgaacctc
    22021 gtacaaacgc ctgcgctttc tccagacgcg tttcataatc aatacgcaac agcatcatga
    22081 tggctgtcga cataatcagt aagctcgaac caccgtaact gatcagcggc aatgtcagac
    22141 ctttggtcgg taacatcccc gccgccgcgc ctacgttaac cagcgcctgg aagctaaacc
    22201 agatgccaat agaacaggcg agaaaaccgg aaaaacggtg gtcaatttct aacgctttgc
    22261 ggccaatcga catcgcgcga aaagcgacga agaataccat taaaagtgcc agcaccacac
    22321 cgacataccc cagttcttcg ccgataatgg cgaaaataaa gtcagtgtgc gcttccggca
    22381 gatactccag tttttgtacc gagttaccta aaccttgtcc ccaaagttcg ccgcgaccaa
    22441 acgccatcag cgattgcgtt aactgatagc cgctgccaaa gggatcttcc cacgggttcc
    22501 agaatgcggt aacacggcgg atacggtacg gttcggcgag tatcagcaac acaaccgctg
    22561 aaatgcccat accgataatg gcaatgaact gccacaattt cgctcccgcc aggaacaaca
    22621 tcgccagcgt agtcacaaac aacaccacca ccgtaccaag gtctggctgt gccagcagta
    22681 acactgccaa caccagaatc acgcccatcg gtttcaggaa gccgcgcagg ttattacgta
    22741 cttcgtcgcc tttacgcacc agatagttgg cgatataaca aaacagcgac agttttgtca
    22801 gctccgcagg ctggatacgc agcaaaccga gatcgatcca acgcgatgcc cctttaaccg
    22861 agctacccac taccagcacg atcatcagca ggatgataga tccgagcagc atcgtggcac
    22921 tgtagcgttg ccagaactcc atcggcagac gcagcgtaat gatcgccaga atgaatgcca
    22981 ggatcagata gacgccatca cgcttcgcga agaagaacgg atcgttggtt aagcgttgcc
    23041 ctatgggcat tgacgccgag gtcaccatga taaagccaat cgccgcaagg ccgaaggtca
    23101 gccacagtaa ggtgcgatcg tacatgatca ggctgtcggt atctttttcc cgcgagccca
    23161 tcacccagcc ttttagcgcc gtggagatcc agaccaggat actgaatcct ggcaggcgcg
    23221 gcattttcag gcgagggaga gataaacgca tcaacctaac tccttcgcca gacgggcaaa
    23281 ctcattgcct cgttgttcaa agttcttgaa ctgatcaagg ctggcacagg ccggggagag
    23341 cagaaccata tcgcccagct gaacacgcgg agccagcaag cgcatcgctt gttccatggt
    23401 ttcggtttgt tctgccactt ccgggcgtag cgccgccagc tgcgcaccgt cacgaccaaa
    23461 acagtacaaa cgcacgttgt cgccattcag atagcgcgcc agtggactga agtccgccga
    23521 tttaccatcg ccgcccagca acaaatgcag cgtgccgtct acctgcaagc cattcaacgc
    23581 cgcttccgta ctgccgacgt tagtggcttt cgaatcgtta atccagcgta cgccattgtg
    23641 ctccagcacc acttcaaagc gatgcggcag accggtgaag gtggttaacg ctttcaggct
    23701 gctggcgcgc ggcaaaccag ctgcgtctgc cagtgccagt gctgccagcg cattggtgta
    23761 gttatgctgc ccggaaagtt tcatctcttt cacattcagc actttctcgc ctttaacccg
    23821 cagccaggtt tcgccctgct gatgattcag gtgatagtca cccatgttga cgccaaagct
    23881 gacgcagcgt tcatcagcac cgcgaatcgg cattgttaag gcatcatcag cattgacgac
    23941 acaaactttc gcattttcgt aaatacgcaa ttttgccgca cggtattgct gcaaaccaaa
    24001 cggatagcgg tccatatgat cttcagtcac gttcagaatg gtcgccgcca ccgcctgtaa
    24061 gctggaagtg gtttccagct ggaagctcga cagttccagc acgtacagtt cgcactcagc
    24121 atcaagcagc atcaacgctg gcaggccaat attgccaccc acaccaacgt taacccccgc
    24181 cgctttcgcc atttcaccca ctagcgtggt gaccgtgctt ttgccgttag aaccggtaat
    24241 cgccacaatc ggtgcttgtg cttcgcgaca gaacagctcg atatcgccaa cgatttcgat
    24301 tccggcatca gcggcagcgc ttaaggatgg atgcgcctgt gcaataccgg gactggcgac
    24361 aatcagatct gccgccatca gccattcatc attcagactg cccgtgtggc gttctacggc
    24421 ttcgggtaat ttatccaggc caggcggtgt catacgcgta tccataacgc gcggcgtcac
    24481 accgcgagcg aggaaaaagt ccacgcagga aagcccggtg aggcccaggc cgataatgac
    24541 gacattttta ccctgataat cagccatgat taacgtacct tcagcgttgc cagaccaatc
    24601 agaaccagca tcagcgaaat aatccagaaa cgcacaatga cgcgcggttc cggccagcct
    24661 ttcagttcat agtggtgatg aatcggtgcc atgcggaaaa tacgttgtcc gcgcagttta
    24721 aaggagccga cctgcaggat gacagaaagc gtttctacca cgaacacgcc ccccataatc
    24781 accagcagga attcctgacg tagcagtacg gcgataatgc ctaacgcacc acctaacgcc
    24841 agcgaaccta catcgcccat aaagacctgc gccggatagg tgttaaacca caggaagccc
    24901 agtcctgccc cgactatcgc cgtgcagaca atgaccagtt cccctgcgtg tcgcagatac
    24961 ggtatatgca agtagctggc aaagttcata ttgccggtcg cccacgccac cagcgcaaaa
    25021 ccaccggcga caaatacggt cggcataatt gccaggccgt cgagaccatc ggtcaggttt
    25081 accgcgttgc cagtacccac aatgacgaag taagccagca gaatgtagaa cagccccagc
    25141 tgcggcatca catctttaaa gaatgggacc accagctgcg ttgcgggcgt gtctttgccg
    25201 gcaaggtaca gggcgaaggc gacacccagc gcaatgaccg acatccagaa atacttccaa
    25261 cgagcgatca accctttggt gtctttacgc accactttgc gataatcatc aacaaagcca
    25321 ataacaccgt aacctaccag caccaccaac acgcaccaga cgtacggatt ggacgggtaa
    25381 gcccacagca gtacggagat cacaatcgcc gtcaggatca taatcccgcc catggtcggc
    25441 gtaccgcgct tgctgaagtg tgattcagga ccgtcgttac gcaccacctg accaaaggaa
    25501 agtttttgca aatgagctat catacgcggg cccatccaca atgagatgaa cagcgcggtc
    25561 agcaggctga cgatggcgcg aaacgtcaga taggaaaaga cgttaaagcc ggaataatat
    25621 ttgaccaaat gttcggccag ccaaactaac atgtcccatt ctcctgtaaa gcgcgtacta
    25681 cctcttccat ggcggcacta cgtgaaccct taactaaaat cgtaattacc tgttgctcag
    25741 caatcagtga tttaagacgc gtaattaacg cagttttatc agcaaaatgt tcgccaacgc
    25801 cgctggcggt gctgatagca tggctttgtt tacccacgct taacacgcgg tcaataccag
    25861 cagcttttgc cgcctcgccc acctgtacat ggcaggcttc gctttcagcg cccagttccg
    25921 ccatatcgcc caccaccagc acgcggtagc ccggcatttc agccagtacc tgaacagcgg
    25981 cggtcattga accgacattg gcgttgtagg agtcgtcgag cagcaactgg ttttctgcca
    26041 gttggatggg gaacagacgg cctggaacag ctttcagatt tgccagcccc gctttgatag
    26101 cattaagcgt tgcgcccacg gacatggaga gcgcagcggc tgccagcgca ttcgcaatat
    26161 tgtgacgccc cggcaacggc agcagaacat cgacgctacc ggttggggtt tgtagggtaa
    26221 attccgtacc gtgcgaggtc acatggatat tggtggcggt gaaatcgctg ttggcggcat
    26281 tgggtgagaa acgccacact ttgcgtgagc caattacgct ctgccagttc agccagtcgt
    26341 tgttgtcggc gttcataatg gcgataccgt tttccggcag gccgctaaag atttcacctt
    26401 tcgctttcgc gacacccgca agcgagccaa aaccttccag atgcgccgct gccaggttgt
    26461 tgaccagcgc agcttccggg cgagtcagac tcacagtcca ggctatttcg ccctgatggt
    26521 tcgcgccaag ttcaataact gcgtaatcgt attccggcgt taagcgcaac aacgtcatcg
    26581 gtacaccgat gtcgttgttg agattgcctg ccgtataaag cgtgttgccg cactggctta
    26641 aaatcgccgc cgtcatctct ttaacggagg ttttgccgga ggaacctgtc agagcaacca
    26701 cgcgcgccgg aacttgctgg cgaacccatg cagccagttc accaaacgcc agacgcgtat
    26761 ccttgacgat taactgcggc aggtcgatat ccagcggacg gctgaccagt agtgcgcccg
    26821 cgccgccagc tttcgcctgg tcggcaaaat catgagcatc gaaacgttcg cctttcaggg
    26881 caacaaacag gcagcccggc gtcagttttc gggtatcagt ggttacagca tcaagggtga
    26941 tatctgcacc ttgcagttca ccgttgagaa tgtcggtaag ttggctaagg gttacgctaa
    27001 tcatgcaatc acccccagca gacgcgccac cgtgacgcga tcggagtagt ccagacgctg
    27061 attgccaaca atctggtaat cttcatggcc tttgcccgcg accagtacca catcattctc
    27121 tttagcctgc ataacggcgc aagtcaccgc ttcagcacgg ccttccatca ctttggcatg
    27181 tccggcatct aacattcccg ccagaatatc gttgatgatg gcacgcggtt cttcggtacg
    27241 cgggttatcg tccgtcacca ccgccacgtc agcaaactct tcggcaattg cgcccatcag
    27301 tggacgctta cctttatcgc gatcgccacc acagccaaag acgcaccaca gcttgcccgc
    27361 acagtgcagg cgcgccgcct gtaaggcttt ttccagtgca tccggcgtat gcgcgtaatc
    27421 caccaccacc gtcggtttgc ctggcgcagt gaacacttcc atacgtccgc aaaccggttg
    27481 cagacgcgcg gcggttttca gcagatcagc cagtggatag ccgagtgcca gcagtgtcgc
    27541 cagcgcgagc agcaggttgc tgacgttaaa agcgcccatc agatggcttt caatttcgcc
    27601 atcgccccaa cttgagctaa agcgaatcgt cgcaccgctg tcgtgatagt tcacgtcgat
    27661 cgcttttaac cagcgtccgt gacagttcgg attaatatga tcttccattg ataccgcaac
    27721 cgcgtccggc agttttgcca gccagcggcg gcccacttca tcgtcggcgt taataatcgc
    27781 ctgaccgcaa tgatgttcag aataaagcag ccatttcgca gcttcgtagt gttccatatc
    27841 accatgataa tcaaggtgat cgcggcttaa gttggtaaag accgacgccg caaatttcaa
    27901 tgccgccaca cggtgctgta ccagcccgtg ggaggaaact tccattgcgc aaaacgtcgc
    27961 gccctgctcc accagccccg ccagctcatg ctgaacatcg actgcggaac cggtagtatt
    28021 ttctgtcggg atcactttcc caagcagacc gttaccaacg gtgcccatta ccgcgctggt
    28081 ttcgccaagc agttggctcc actgcgccag cagctgggta gtcgtggttt tgccgttggt
    28141 gcccgttacg cctacgagac gtaaattgtc agagggttca tggtaaaagc ggcccgccag
    28201 tgcagataaa cgctcattga gctggctgag atagatgacc ggtacgccgt gcatttcacg
    28261 gatttcaccg tcggtcgcct catctttcgc tttcgcctct gcaataatgg cagccacacc
    28321 ttgcgctatc gcctgcggga tatatcgacg cccgtccgcc tgatgaccta ctacagctac
    28381 aaagagatcg cccgccgcag ccacacggct gtcgagtgtc atctctcgca gtgctcgcga
    28441 aggtgcgtct ggcacccacg gagcaagaag gtcgcgcaaa ttacgatctg ccacctgtcc
    28501 cctcgccttg attaatcaca aattcatttt tatcgcccgt tgtcagcgca tccggctcga
    28561 tgttcatggt acgcaatacg ccgcccatga tggcaccaaa gaccggcgcg gaaacggcgc
    28621 cgccgtagta tttacccgcc tgcggatcgt tgataacaac aaccagcgcg aagcgcggct
    28681 gactcgcagg cgcaacgcct gcggtataag caatatattt attgatgtag cgaccgtccg
    28741 gcccgacctt tttcgcggta ccggttttaa tggcgatacg atagccttta atcgccgcct
    28801 tcacgccgcc gccgcctggt agcgccacgc tttccatcat atgcaccaca gtgcggacaa
    28861 tggatcccgg gaagacacgt tcaccgggaa ccggggggtc aactttggta atcgacagtg
    28921 ggcgataaat gccgtagctg ccgatagttg cgtagactcg cgctaactgt aacggtgtta
    28981 ccattagccc gtagccgaaa gagaaggtgg ccctctctat gtcagaccac cgttgttttt
    29041 gaggatataa gccactgcgt tctccgacca accccaaatt ggtcgctttt cccagtccaa
    29101 aacgtgagta agtatctact aacgctgagg acggcatcgc taacgccagc ttggaaacac
    29161 cgacgttact cgacttctgt aataccccgg tcagggttaa ttcgctgtag cgtgccacgt
    29221 ctttgatttc gtggccgtta attcgataag gaatggtatt gagtaccgag ttttcccgca
    29281 ccacgccacg ttgcaacgcg gtcattacca ccatcggttt aaccgttgag cccggttcaa
    29341 acacgtcggt gatggtacgg ttacgcatcg cctctttcgg cgtgccgctc agattgttag
    29401 ggttgtatga cgggctgtta gccatcgcca gcacttcacc ggtgttgaca tccaccagca
    29461 cggcgctacc agattcagcc ttgttaaagg ccaccgcgtt gttcagttcg cgataaacca
    29521 gcgcctgcag gcgttcatca atactcagcg ccaggttgtg cgctgcctgg ctgtcagtag
    29581 aagaaatatc ttcaattacg cgaccatagc ggtctttacg cacaatgcgc tcacccggct
    29641 gcccggtaag ccatttatcg aaactcttct caacgccctc aatcccttga ctatcgacgt
    29701 tagtaaagcc gatgaggtga gcagtcactt cgccggacgg atagtaacgg cgagactctt
    29761 cacgcagatg aatccccggc agtttcagtt ttttgatgta gtccgccatg tcagggttca
    29821 cctgacgcgc cagataaata aagcgccctt tcgggttggc gttaatgcgg gctgaaagct
    29881 gatccagcgg aatattgagc gcgttagcca gcgccttcca gcggtcaccg acgctgatac
    29941 cgccagcgtc atgcacttct ttcgggtcag cccaaatcgc ttttaccggc acgctcaccg
    30001 ctaacgggcg accagaacgg tcagtaatca tgccgcggga ggtggaaact tgctgaacgc
    30061 gaagagaacg catgtcgccc tctttcacca gcatatccgg ggagataact tgtaaccacg
    30121 ctacgcgtcc gagcagaaaa gccagcgcca ggagaataca gccgcataac aacgcaaaac
    30181 gccaactgat aaagttggca tgttcttcct gacgttttgg tttctgcgtt ttcgccgctg
    30241 ctttcatgcg tcgcgtttat ccttattttt gcactacgat attttcttgt gacggatcaa
    30301 catgctgcat ttgcagcttt tccgtggcga tcctttccac ccggctatgg tcgccgagcg
    30361 cattctcttc aaggatcagg ttgcgccatt caatgtctaa agcatctcgc tccagcacca
    30421 gttgttcgcg ctgagcggtc agtaaacggg tatggtgcgc cgtggttacc acagtcaccg
    30481 ccgtcaaaat aatgcaaatg aacaggcaga gtggcagctt cccaaatcgc aaaagatcgt
    30541 caccgataac accaggcaat gcatggcgct cgtggcttcc catcgatcct ttaactttgc
    30601 ttagagcttc tgtcactctg ctgatcatgc attcgtcctc tctgcaatac gcagaactga
    30661 actacgggca cgagggttct cagccacctc ttcttcgccc ggcattaact tgcctagtgc
    30721 tcgcagctga cggccaccca gttttttgag ctgctcttca gtcatcggta accctgccgg
    30781 aacttgcgga ccgcggctgt tttcacgcat aaaacgtttc acaatacggt cttccagcga
    30841 gtggaagctg atgatcgaaa gccgcccacc cggggccagc acgttgagcg agctttttag
    30901 cgcctgctct atctcctcca gttcactgtt tacccaaatg cgcaccgcct ggaaggtacg
    30961 ggtcgcggga tgtttaaact tatctttcac cggcgttgca acagccacga cttccgccag
    31021 ttctttggtg cgggtcatcg gctgttcgcg gttacgctcg acaatggcgc gggcaatgcg
    31081 tttggcaaaa cgctcttcac cataggtttt caatacccaa gcgatatcgg cttcttctgc
    31141 ggtttgtagc cattcagcgg ctgactgccc acgggttggg tccatacgca tgtccagcgg
    31201 accatcgcgc ataaaggaaa agccgcgctc agcatcatca agttgcggtg aagagacgcc
    31261 aagatcgagg agaatgccgt cgatcttgcc gataagatcg cgctcggcaa cgtattcgcc
    31321 cagcgcggag aaaggtccgt ggatgatgga gaagcgcgga tcatcaatag tcttcgcaac
    31381 ggcgatagcc tgcgggtcgc gatcgatcgc cagcaaacgc ccctcttcgc caagctgcga
    31441 gaggatcaga cgtgagtgac caccgcgacc aaaagtccca tcaatgtaga tgccatcagg
    31501 acggatattg aggccattaa cggcttcatc cagcagcacc gtagtatgtt tatagttttc
    31561 catcatttta tagagacaag tcctgcagtc gctccgataa gtctccggta gccaactgct
    31621 ctgcgtcgat atcttccttg acctgttgat gccaggttgt ttcatcccac agctcaaact
    31681 tgttgaactg tccaaccagc atcacttctt ttgtcagccc ggcatgttgc cgcagtactg
    31741 gcgcgattaa caatcgacct gcgccatcca tctgacattc gctggcatga cctaacagta
    31801 ggcgctgcac acggcgctca accgggttca tgctcgacag acgcgataat ttttgctcga
    31861 taatttccca ttcaggcagg gggtaaagca gcaggcacgg gtgatgaatg tcaatggtgc
    31921 aaaccatttg accgacagcg ttctcaagca gctgttcccg gtaacgggta ggcactgata
    31981 agcgcccttt gctgtcgaga ttgactaacg ttgctccccg gaacatgcca gcctcacccc
    32041 ttattcccac tttgccccac aatttcccac tgaaaggagt ttacggagcg gaggaaaagc
    32101 ttgtcaagcc agtcacaagg cataccgagg taaaaaaccc gatgtttacg gctaatatca
    32161 gcgttgttta ttttacgtcc aacgtgcgca gcaaattaac gttatgaata gctgtaagaa
    32221 aaaatccctc atcgtcatca ccgttttaaa atgactcact attttccaca gcaaatataa
    32281 agtgtcagtt tgcgacgcga gcggcatttt aggacatatc ttccccggtt aacagtccct
    32341 gttgcgtctg tgtggcgctt gatgccagcg ggtttgcgca aaagcaaaga aaagtgcttg
    32401 ttaaatcggc aattcatctc tgcatgtaac aaaataatgc aaaaacgcgc tgtcgatatc
    32461 acttaattat tgtgaatttc agctggtgag ttaattaacg ggcgggttta aaagaactta
    32521 tcaataatag tcaggaatag tcttatttac tttaaggata ttgatgtcca ttcccgtact
    32581 ctacg
//
LOCUS       DAZPDU010000031        31225 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZPDU010000031 DAZPDU010000000
VERSION     DAZPDU010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31225
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.031"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..559)
                     /locus_tag="ACHURS_001320"
     CDS             complement(<1..559)
                     /locus_tag="ACHURS_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFR1237099.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVLLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQR"
     gene            complement(762..998)
                     /gene="feoC"
                     /locus_tag="ACHURS_001321"
     CDS             complement(762..998)
                     /gene="feoC"
                     /locus_tag="ACHURS_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFR1237100.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            complement(998..3319)
                     /gene="feoB"
                     /locus_tag="ACHURS_001322"
     CDS             complement(998..3319)
                     /gene="feoB"
                     /locus_tag="ACHURS_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFR1237101.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTSARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            complement(3336..3563)
                     /gene="feoA"
                     /locus_tag="ACHURS_001323"
     CDS             complement(3336..3563)
                     /gene="feoA"
                     /locus_tag="ACHURS_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFR1237102.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            complement(4001..6322)
                     /locus_tag="ACHURS_001324"
     CDS             complement(4001..6322)
                     /locus_tag="ACHURS_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFR1237103.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKVAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFKNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(6419..6895)
                     /gene="greB"
                     /locus_tag="ACHURS_001325"
     CDS             complement(6419..6895)
                     /gene="greB"
                     /locus_tag="ACHURS_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFR1237104.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVNYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            7123..7842
                     /gene="ompR"
                     /locus_tag="ACHURS_001326"
     CDS             7123..7842
                     /gene="ompR"
                     /locus_tag="ACHURS_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFR1237105.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            7839..9191
                     /gene="envZ"
                     /locus_tag="ACHURS_001327"
     CDS             7839..9191
                     /gene="envZ"
                     /locus_tag="ACHURS_001327"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFR1237106.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(9267..10889)
                     /gene="pckA"
                     /locus_tag="ACHURS_001328"
     CDS             complement(9267..10889)
                     /gene="pckA"
                     /locus_tag="ACHURS_001328"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFR1237107.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKLDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            11268..12968
                     /locus_tag="ACHURS_001329"
                     /pseudo
     CDS             11268..12968
                     /locus_tag="ACHURS_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
     gene            complement(13103..13981)
                     /gene="hslO"
                     /locus_tag="ACHURS_001330"
     CDS             complement(13103..13981)
                     /gene="hslO"
                     /locus_tag="ACHURS_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFR1237108.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(14006..14407)
                     /gene="hslR"
                     /locus_tag="ACHURS_001331"
     CDS             complement(14006..14407)
                     /gene="hslR"
                     /locus_tag="ACHURS_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFR1237109.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(14418..15086)
                     /gene="yrfG"
                     /locus_tag="ACHURS_001332"
     CDS             complement(14418..15086)
                     /gene="yrfG"
                     /locus_tag="ACHURS_001332"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFR1237110.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(15151..17286)
                     /gene="igaA"
                     /locus_tag="ACHURS_001333"
     CDS             complement(15151..17286)
                     /gene="igaA"
                     /locus_tag="ACHURS_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFR1237111.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDGSRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            17606..18166
                     /gene="nudE"
                     /locus_tag="ACHURS_001334"
     CDS             17606..18166
                     /gene="nudE"
                     /locus_tag="ACHURS_001334"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFR1237112.1"
                     /translation="MSKSLQKPTILNVETVAHSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(18331..20883)
                     /gene="mrcA"
                     /locus_tag="ACHURS_001335"
     CDS             complement(18331..20883)
                     /gene="mrcA"
                     /locus_tag="ACHURS_001335"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFR1237113.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMDGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGVGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            21003..21782
                     /gene="hofM"
                     /locus_tag="ACHURS_001336"
     CDS             21003..21782
                     /gene="hofM"
                     /locus_tag="ACHURS_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFR1237114.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQRLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRCRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            21782..22321
                     /gene="hofN"
                     /locus_tag="ACHURS_001337"
     CDS             21782..22321
                     /gene="hofN"
                     /locus_tag="ACHURS_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFR1237115.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTD
                     NAEARVNTVLLQAEQQLARSLQITKPRLLERQQIREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            22305..22745
                     /gene="hofO"
                     /locus_tag="ACHURS_001338"
     CDS             22305..22745
                     /gene="hofO"
                     /locus_tag="ACHURS_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFR1237116.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            22735..23139
                     /gene="hofP"
                     /locus_tag="ACHURS_001339"
     CDS             22735..23139
                     /gene="hofP"
                     /locus_tag="ACHURS_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFR1237117.1"
                     /translation="MRVKRWLLAGIVLCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            23051..24289
                     /gene="hofQ"
                     /locus_tag="ACHURS_001340"
     CDS             23051..24289
                     /gene="hofQ"
                     /locus_tag="ACHURS_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFR1237118.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            24690..25211
                     /gene="aroK"
                     /locus_tag="ACHURS_001341"
     CDS             24690..25211
                     /gene="aroK"
                     /locus_tag="ACHURS_001341"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFR1237119.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            25268..26356
                     /gene="aroB"
                     /locus_tag="ACHURS_001342"
     CDS             25268..26356
                     /gene="aroB"
                     /locus_tag="ACHURS_001342"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFR1237120.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            26448..27734
                     /gene="damX"
                     /locus_tag="ACHURS_001343"
     CDS             26448..27734
                     /gene="damX"
                     /locus_tag="ACHURS_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFR1237121.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPTATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETARNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            27841..28677
                     /gene="dam"
                     /locus_tag="ACHURS_001344"
     CDS             27841..28677
                     /gene="dam"
                     /locus_tag="ACHURS_001344"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFR1237122.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKTYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            28695..29372
                     /gene="rpe"
                     /locus_tag="ACHURS_001345"
     CDS             28695..29372
                     /gene="rpe"
                     /locus_tag="ACHURS_001345"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFR1237123.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            29365..30123
                     /gene="gph"
                     /locus_tag="ACHURS_001346"
     CDS             29365..30123
                     /gene="gph"
                     /locus_tag="ACHURS_001346"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFR1237124.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPIAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            30116..31120
                     /gene="trpS"
                     /locus_tag="ACHURS_001347"
     CDS             30116..31120
                     /gene="trpS"
                     /locus_tag="ACHURS_001347"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFR1237125.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
BASE COUNT         7309 a         7971 c         8448 g         7497 t
ORIGIN      
        1 cgcgttgacg aatatgtttt tgaattaact ccagcagcgc catacgtcgg tgctgcatga
       61 tttcatcatc aggcatgacg gtgacatcaa tcagcggaaa tgcggaggca taaagctgcc
      121 ttgccagttt gggatcggcg aaacaatcca gccaacacag cgaataggga taggggcttt
      181 caataccgtg gtaaaacaac aatagcacca ccatcggcaa cgttttgtat ccagcatcaa
      241 ggtgattttg cattgcggca atagcgtaac gcatcatgcg aaatgcgatc agtttgtttg
      301 aggtgctttg atgttcaatc agacaataga tgtatcctgg tccttgttcc gttttcaccg
      361 accacagcac atcggaatag ctttcacgca gatcgtcatc aataaagctg ctcgactcca
      421 gcttgagggt tttcatatca caaagcgcgt gaatgggtgc cggtaaatga aacgcaagaa
      481 aatcacgagc cgtgtccggt tggcgtaaaa agagtttgaa cagcgcatcg tgtggggtgg
      541 aactctgctt tttgctcatg gcgttccttc acctgttgtt tgaatgccgc gactctacgc
      601 gccactccac acctgtgcat tagcgagttt tcatccctgc gagatgcttt caaaggaact
      661 tacgccgtgc tgcaagagtt gcatttttat ggaaagccgc acgcaaaaaa gcccggatgc
      721 agacgcatac cgggctttcc aaaacaggcg atggagtaag gttaacgcag cgcccaccac
      781 tcgcgcagac aggcttttcc ttccgggcag cttttacaac tgccagagag gcagccgtca
      841 ggttcttcct gaatccgcac ggctttgccc atactttcca gttgttgcag catggcgtta
      901 atcattggct gcggagtgtt caatgtctgg cttatctggg tcgcttccat acggccccgt
      961 aacgccagca aatcacgtac ctgaataagt gaagccatta atggcaatca ccggtggtgc
     1021 tggctgcgca gcaactgctt accgacttgc gggttgccag cagttcgata tccacccggc
     1081 tacgcgcgcg gcgcagcaga ccgataacta cgatgttaaa caggattacc gccagaatgc
     1141 acaccaggct gtaagttggg tgctgactgt agctggcgac ctgatagaac aatgttgcca
     1201 gtgagtaagc gatattcagc ccccacagga tggagaagcc catccagcca cggcttgatt
     1261 cacgggcgat ggctcccatc accgagatac atggtacata cagcaggacg aaaatcaggt
     1321 agctgtatgc ggcagctgcg ctaccgaatt tctgatccat cacgcccatc gccccggtac
     1381 ccatttcacc gtcgcctttg ctagcttcaa tggggttcat cagtacgcta aggctgaagg
     1441 tgtctttcag gctctgccag gtttcatcta ccgcactgaa cagctcttca ccgaggttga
     1501 attccgccgg attgaactct tcgtcctgaa tattttctgc ggtgtagagg gtgttgagcg
     1561 tacccaccac cacttctttt gccattgcgc ccgtaaacag gccaaccgtt gcctgccagt
     1621 tatcttcatg tacgccaatc ggcttgaaga caggggttat cacccggctg acggacgcca
     1681 gcgccgagtc gttgatgtta tcgacgattt tcccgctcaa cgagaagctg ttgaaagcgc
     1741 tcaggaaaat gctgacgatg ataatcactt taccagcacg cagaacgaag cccttcagac
     1801 gctgccaggt ctggataatc aggcttttaa cgtgtggtac atggtagacc ggcagctcca
     1861 tgacaaacgg cgtcgcttca ccgcgcatga tggtgtactt gagcatcagg ccagtcagca
     1921 ccgccatcac gataccgagc atatacagcg agaaaaccgc cagggcaccg ttctgcccga
     1981 agaaggcagc cgcgaatact gcgaagatag ccagacgcgc gccgcaggac ataaacggtg
     2041 ccatcatgat ggtcatcaga cgttcacgcg gtgcatcaag cgtacgtgcg cccattaccg
     2101 acggtacgtt acaaccgaaa ccgacgatca gcggcacaaa ggatttcccc ggcaagccca
     2161 gcgcctgcat caggcggtcc atcacaaacg ctgcacgcgc catatagccg gagtcctcaa
     2221 ggaaggagag gaacaggtac atcatgccga tctgcggcac cagtggcagc acggtgttaa
     2281 taccgccacc caggccctgg gcgaggaaga tagtcagcca gtccgggaag tggagcgtgt
     2341 agccaatcca ttgaatacca tgcacaaata gcgccacgga gccgacgtca aacagcggct
     2401 gtaacgcccc gccgatgttg atagccagca ggaacatcag gtacatcaca aagaggaaaa
     2461 tcggcagacc gaggaaacgg ttgagcacga ttttatctac cgcagtggtg aaacggctgg
     2521 gttctgccgt cagggtgttg cttaccacat cacagatggc agctatgcac tggtaacgcg
     2581 catcggcaat gtgcagcgcc ggatcatcca tttcattacg cagacgggca agggcggcat
     2641 ccagatgctg cgacgcttca ccggcgtagg cgcggctgta gatatcgcct tccagcattt
     2701 gcaggcccag ccagcgacgt tgtttcagcg ggatgtcgga aggcatcact tttgccagtg
     2761 aatctgcttc gttgagtagc ggctgtgcgt aatgcaccag ttccacattc tcgttagctt
     2821 tatagcgatc aatcgccagc ttgagcgctt caataccgcg accacgggtt gaaaccagcg
     2881 ggatcactgg gcagccaaga cgcgccgaca gagcatcaat ttcaatacga atattttgct
     2941 tctcggcaat gtcgagcatg ttcagtgcca cgatgcaggg aatgccgagt tccagcagtt
     3001 gtagcgtcag gtacaggtta cgctcaaggt tagacgcatc caccacgtta atcagcaggt
     3061 cggcgtcgcc actcaaaatg tagtgacagg cgatttgctc atcgagcgag gtctgcgatg
     3121 agatggtggt cagagaatag gtgccgggca ggtccaccag cgtgacctga tgatcggtgg
     3181 tggagaattg cccttcttta cgttcgacgg taacgccagc ccagttacct acacgctgac
     3241 gtgcgctagt gagctggtta aataaggttg tcttgccaga atttggatta ccaattaagc
     3301 caatggttaa ttttttcatt gttgttatca ccgtattaac aggaaaccgc ttccacttct
     3361 aataaggcca gatctttttt gcgtaatacc aggctcacac gacgggtttc gatatgaatg
     3421 gggtcgccga gtggagcgac gcgcaccaca ttaaaagagg agccaggtaa catgccaaga
     3481 gaaagcagtt tttggcgata tgccgggctg atttcacggg aaaagccagt gattttccac
     3541 gcagtatctg gagtgtattg cataggtgcc tacttgtttc tcattaactg gataactacc
     3601 tcatggggcg tttgacagcg acatgagacc agatgatttg ccaacaaagg agagattaaa
     3661 taatcaacaa cacaatgata atgagaatgg tttctatcag caatacttaa aatgtgtgcg
     3721 aatgttggtt tgaataataa tctgactcaa tgttgatgtg gctcaaggtt atatcgcagc
     3781 gaaaatataa ttcgaatagt gaattacccg gactggctaa tgtttaatta aggtttcagt
     3841 tgttaatgaa gtgataaata ttgccggaat atattatgag tttggttgtg gaataaaaag
     3901 gccggagata ttctccggca cttttatgtc aggcaaggcg ttcgttgcca gatgcggcgt
     3961 gaacgcctta tcgcgcaggt ttaggggcca gtccgggcgt ttaacgtttt ttgcccattg
     4021 ccgccgccag cgcatccatc atcgcgctat taccggcagg ctgcgcttca cgaccgcgtg
     4081 gtttggctgc cgggcggttg ttttgcgggc gttcattacc gccgccgcga cgggcgttgg
     4141 tttcgccagg ctgctcgtcc agacgcatgg tcagggcgat acgtttacgc tgaagatcca
     4201 cttccagcac tttcaccttc acaatgtcgc ccgctttcac cacggtatgc ggatcttcca
     4261 caaacttgtt cgacaatgaa gagatgtgaa ccaggccgtc ctgatgcacg ccaatatcga
     4321 caaacgcgcc aaagttggtg acgttggtga cagcgccttc gaggatcata cccggttgca
     4381 ggtcgttcat tgtctcgacg ccatcggcaa actgagcggt tttaaattcc ggacgcggat
     4441 cgcgacccgg tttttccagc tctttgatga tgtcagttac tgtcggcaca ccgaattttt
     4501 catcagtaaa gtcagacgct ttcaggttac gcagttcgct gctgttaccc atcagatctt
     4561 tcagtgcctg ctgtgttgct gccagaatgc gttccaccac cggataggct tccgggtgaa
     4621 cggtagaggc gtccagcggg ttatcaccgt ggttaatgcg caggaagccc gcgcactgct
     4681 cgaaggcttt cggccccaga cggctgactt tcagcagttg ctgacggttc ttgaactggc
     4741 cgttctcatc gcgccaggcc acgatgtttt gcgccatcat gcgcgtcagg cccgccacgc
     4801 gagttaatag cggaacagaa gcggtgttga gatcgacgcc aacggcgttt acgcagtctt
     4861 ctactactgc atccagtttg cgggccagtt gcgtctggct gacgtcatgc tgatactgac
     4921 ctacgccgat agatttcgga tcgattttca ccagctccgc cagcggatcc tgcaaacggc
     4981 gggcgataga taccgcacca cgcagcgaaa cgtcgagatc cgggaactcc tgcgctgcca
     5041 gctcggaagc tgagtaaacc gacgcgccag cttcgctgac gatcactttc tgtgcggtca
     5101 ctttcgggaa ctgcttctgc acgtcgagat agaaacgttc agtttcgcgg gaagctgtac
     5161 cgttgccgat cgccaccagt tcaacgttat gtttttcaca caaggcagca acggtcatcg
     5221 ctacttttgc ggcttgtccg gtgtgcgggt aaatggtatc ggtcgccacc agtttgccag
     5281 tggcatcgac cactgccact tttaccccgg tacgcagacc cggatcgagg cccatcgttg
     5341 cacgcagtcc ggcaggggcc gccatcagca gatcgtgcag gttacgggca aagacgttga
     5401 ttgcttcatc ttccgcgcgt tcgcgcacgg tacccatcag ttcggtttcc agatgcatca
     5461 gcaccttgat gcgccaggtc cagctcacta cgcctttgcg ccagctatcc gccggggcat
     5521 tgttcaggcg caggccaagg tgatccatga tgatttgctc gcaatagctc tctttgggcg
     5581 gctcatcgaa ctgcggatcg gcattcagcg aaagctggag tacgccttcg ttacgcccac
     5641 ggaacatcgc cagcgcgcgg tgagaaggca ccgtggacaa cggttcgtga tgatcgaaat
     5701 agtcgcggaa tttcgcccct tcctcttctt taccgctcac caccgtagaa accaaatgcg
     5761 cgttcttcca cagataatca cgcactttcg ccagcagcgc ggcatcttcg gcaaaccgtt
     5821 ccatcaggat atagcgcgcg ccgtccagcg cggctttggt atctgccacg cctttatcgg
     5881 catcaatata ttgcgcagcg gcgacttctg gtgtgtgtga cggatcgctc cacagcaggt
     5941 cagccaacgg ctcaagccct gcttcaatgg cgatttgccc gcgggtgcgg cgtttaggtt
     6001 tgtagggcag gtagaggtct tcgagttcgg ttttgcttag ggtggcgttg atggccttcg
     6061 ccagatcatc ggtgagtttg ccttgctcgg aaatggactt gaggatcgcc tgacgtctct
     6121 cttccagctc gcgcagatag ctcagacgcg tttccagatt acgcagctgc gtgtcatcca
     6181 gaccgccggt gatttcctta cgataacgtg cgataaacgg cacggtattc ccttcgtcaa
     6241 gcaggcgaac ggcagcgtca acctgttccg ggcgcgcctg aatttcaccc gcaataatgc
     6301 ggcagaacga atcattcatc atggtttgag ttcatctttt cggatcaaaa atcaggggat
     6361 agttatacgg actggctggc aaaaatgcca gccatcggca ggaggttaag actcttcatt
     6421 acggtttcac gtattcgata gcattaacat accagctcgc ttccccggca ggggtattca
     6481 ccaccgccag atcgccgact tcttttttca gcaatgcgcg ggccatcggg gaatcgatag
     6541 agatgtaatc tttacggcca aaaatttcat cgtagccgac aatacggaaa cggtgagtca
     6601 cgccatcgtc gttttcaatc tccacccacg cgccaaaaaa gactttgcct tcctgctgag
     6661 gggagtaatt gacgattttg agattttcca tgcatttagt gagatagcgc acgcgacggt
     6721 cgatttcacg cagacgcttt ttattatact gatagtcagc attttcgctg cggtcgccca
     6781 gacttgcggc ccaggtcacc ttttttgtga cctccgggcg ttcttcacgc cagagataat
     6841 taagctcttg tttgagtttt tcataccctt cccgggtaac caggggcgtt ttcatctcgt
     6901 tgattccctt tgtctgtttg ataatgcgca cattgggtat aacgtgatca tatcaacaga
     6961 atcaataatg tttcgccgaa taaattgtat acttaagctg ctgtttaata tgctttgtaa
     7021 caatttaggc tgaaattcat accagattta gctggtgacg aacgtgagct tttttaagaa
     7081 tacacgctta caaattgttg cgaacctttg ggagtacaaa caatgcaaga gaactacaag
     7141 attctggtgg tcgatgacga catgcgcctg cgtgcgctgc tggaacgtta tctcaccgaa
     7201 caaggcttcc aggttcgaag cgtcgctaat gcagaacaga tggatcgcct gctgactcgt
     7261 gaatctttcc atcttatggt actggattta atgttacctg gtgaagatgg cttgtcgatt
     7321 tgccgacgtc ttcgtagtca gagcaacccg atgccgatca ttatggtgac ggcgaaaggg
     7381 gaagaagtgg accgtatcgt aggcctggag attggcgctg acgactacat tccaaaaccg
     7441 tttaacccgc gtgaactgct ggcccgtatt cgtgcggtgc tgcgtcgtca ggcgaacgaa
     7501 ctgccaggcg caccgtcaca ggaagaggcg gtaattgctt tcggtaagtt caaacttaac
     7561 ctcggtacgc gcgaaatgtt ccgcgaagat gagccgatgc cgctcaccag cggtgagttt
     7621 gcggtactga aggcactggt cagccatccg cgtgagccgc tctcccgcga taagctgatg
     7681 aaccttgccc gtggtcgtga atattccgca atggaacgct ccatcgacgt gcagatttcg
     7741 cgtctgcgcc gcatggtaga agaagatcca gcgcatccgc gttacattca gaccgtttgg
     7801 ggcctgggct acgtctttgt accggacggc tctaaagcat gaggcgattg cgcttctcgc
     7861 cacgaagttc atttgcccgt acgttattgc tcatcgtcac cttgctgttc gccagcctgg
     7921 tgacgactta tctggtggtg ctgaacttcg cgattttgcc gagcctccag cagtttaata
     7981 aagtcctcgc gtacgaagtg cgtatgttga tgaccgacaa actgcaactg gaggacggca
     8041 cgcagttggt tgtgcctccc gctttccgtc gggagatcta ccgtgagctg gggatctctc
     8101 tctactccaa cgaggctgcc gaagaggcag gtctgcgttg ggcgcaacac tatgaattct
     8161 taagccatca gatggcacag caactgggcg gcccgacgga agtgcgcgtt gaggtcaaca
     8221 aaagttcgcc tgtcgtctgg ctgaaaacct ggctgtcgcc caatatctgg gtacgcgtgc
     8281 cgctgaccga aattcatcag ggcgatttct ctccgctgtt ccgctatacg ctggcgatta
     8341 tgctattggc gataggcggg gcgtggctgt ttattcgtat ccagaaccga ccgttggtcg
     8401 atctcgaaca tgctgctttg caggtcggta aagggattat tccgccgccg ctgcgtgagt
     8461 atggcgcgtc cgaggtgcgt tccgttaccc gcgcctttaa ccatatggcg gctggtgtta
     8521 agcaactggc ggatgaccgc acgctgctga tggcgggggt aagtcacgac ttgcgcacgc
     8581 cgctgacgcg tattcgcctg gcgactgaga tgatgagcga gcaggatggc tatctggcag
     8641 aatcgatcaa taaagatatc gaagagtgca acgccatcat tgagcagttt atcgactacc
     8701 tgcgcaccgg gcaggagatg ccgatggaaa tggcggatct taatgcagtg ctcggtgagg
     8761 tgattgctgc cgaaagtggc tatgagcggg aaattgaaac cgcgctttac cccggcagca
     8821 ttgaagtgaa aatgcacccg ctgtcgatca aacgcgcggt ggcgaatatg gtggtcaacg
     8881 ccgctcgtta tggcaatggc tggatcaaag tcagcagcgg aacggagccg aatcgcgcct
     8941 ggttccaggt ggaagatgac ggtccgggaa ttgcgccgga acaacgtaag cacctgttcc
     9001 agccgtttgt ccgcggcgac agtgcgcgca ccattagcgg cacgggatta gggctggcaa
     9061 ttgtgcagcg tatcgtggat aaccataacg ggatgctgga gcttggcacc agcgagcggg
     9121 gcgggctttc cattcgcgcc tggctgccag tgccggtaac gcgggcgcag ggcacgacaa
     9181 aagaagggta aataaacggg aggcgaaggt gcctcccgtt ttgctttcta taagatactg
     9241 gatagatatt ctccagcttc aaatcattac agtttcggac cagccgctac cagcgcggca
     9301 cccgcagggg tgtcggtgta tttatcgaag ttgtcgataa acagtttcgc cagggtttcg
     9361 gctttttcct gccactgttc cggagaagcg taggtgttac gcggatcgag aatcttcgtg
     9421 tctacgcccg gcagttcggt tgggatcgcc aggttaaaca tcggcagagt gaaggtttct
     9481 gcattatcca gcgaaccgtt gaggatggcg tcgataatgg cgcgggtatc tttaatcgag
     9541 atacgtttgc cagtgccgtt ccagccagtg ttaaccagat aagcctgcgc gcccgccgcc
     9601 tgcatacgtt tcaccagcac ttctgcgtac tgagtcgggt gcagcgacag gaacgccgcg
     9661 ccgaagcaag cggagaaggt tggcgtcggt tcggtgatgc cacgctcagt accggccagt
     9721 ttggcggtga agccagagag gaagtgatac tgggtttgat cggcagtcag gcgagaaacc
     9781 ggcggcaaca cgccgaaagc atcagcagtc aggaagataa ccttagtcgc gtggcccgct
     9841 ttggaaaccg gcttaacgat gttatcgatg tgatagattg gataagaaac gcgggtgttc
     9901 tcggtttttg aaccatcatc aaagtcgata gtgccatctt cacgcacggt gacgttttcc
     9961 agcagcgcat cacgacggat agcgttgtag atttcaggtt ccgcttcttt cgacagcttg
    10021 atagtttttg cgtagcagcc gccttcgaaa ttaaacacgc cgtcatcgtc ccagccgtgt
    10081 tcgtcatcgc caatcaggcg gcgtttcggg tcggtggaaa gggtggtttt accggtgccg
    10141 gaaaggccga agaacaccgc aacatcgcct ttctcgccaa cgttggcgga gcagtgcata
    10201 gaagcgatac ctttcagcgg cagcaggtag ttcatcatcg agaacatccc tttcttcatt
    10261 tcgccgccgt accaggtgcc gccaatcagc tgcatgcgct cggtcaggtt aaacgccacg
    10321 aagttttcgg agttcagacc ctgttctttc cactgcgggt tagtgcactt cgcgccgttc
    10381 ataacgataa agtctagttt gaaacctgcc agctcttcat cgctcgggcg aataaacatg
    10441 tttttgacaa aatgcgcctg ccaggccact tcggtgatga aacggacgga aagacgagta
    10501 tccgggttcg caccacagaa agcgtcgaca acgaacagac gtttgccgga aagctgcctg
    10561 gtcaccaggc ctttcagatg ctgccaggtt tccggagaga gaggtttgtt gtcgttctta
    10621 cctttgcctt tgtctgccca ccagaaagta tcgcgagtgg tatcgtcacg gacgatatac
    10681 ttatcttttg gtgaacgacc ggtgaagatc ccggtatcga cggcaacggc acccagatta
    10741 gttaacaccc cgcgctcata acctgtcagg ctcggatcga gctcttcctg atacagcagg
    10801 tcgtagcttg ggttgtaaac gatatcatgt acgtcactga taccataagc ctcgagttct
    10861 tgcggggtca aaccattgtt aacgcgcatt tcactgctcc ttagccaata tgtattgcct
    10921 gaatagtaaa gtctttttgg gggtgttaac cgcgacaagg ctcatagatt tacgtatctg
    10981 gagaaattca tatttgacaa aaaactccgt gattcctgtc acgaaacggt tgctattatc
    11041 gcagaaatag cattttaggt gtggaaaatg ttctcaaaag gttaaatctt ggtttatatg
    11101 gcgggaatat tgtgagtgga atcgcattcc tgaatgaaat tgatgcaaat tcaaccggta
    11161 aagtatcgat tcaccacgct aacttttttt gattattttc tgaaaaaagc aaaatactac
    11221 tgcccggatg cgataattaa cccacctttt gattctggat aaccaacatg gataacgttg
    11281 aactttcacc ggcaacacgc tgggggatga ttgctaccgg attacttcag gggctggttt
    11341 gctacctgct tatcgcctgg cttgccggaa aaaatcacag ttggattgtt tatggcgtgc
    11401 ctgcgaccgt cgcgttttca tccgttttac tcttctccgt gatctctttt aaacagaagc
    11461 gcctttgggg atggctggcg ctggtgttta ttgccacgac agggatgagc ggttggctga
    11521 agtggcaaac tgatggcatg aacccctgga gagctgaaaa ggcgatttgg gattttggct
    11581 gatatctgct gttgatggca atgttgttgc taccgtggat acaacaaagc cttcgtatcc
    11641 gcaatggcag tagccgctat agctactttt accagtcagt atggcataac gtacttatat
    11701 tactggtgat ttttctctcc aatggcctga cgtggctggt gcttttactg tggggtgagt
    11761 tgtttaaact tgttggcatc acgtttttca atacgctctt ttttgcaacc gactggttta
    11821 tgtatcttac atttggtctg gttaccgcac tggtggtgat cctcgcgcgt acacagtcac
    11881 gtttaatcga ctctattcaa aagttgttca cgctaatcgc cacggggttg ctgccgttag
    11941 tatcattgct aaccctgatg tttatcatca ccctgccgtt tacggacctg agcgcgattt
    12001 ctcgccacat ctccgccgcc gggttgctgt taacgctggc ctttttgcaa ttgatcttaa
    12061 tggctattgt ccgcgatccg caaaaagcgt cacttccctg gacggggccg ttgcgttgcc
    12121 tgattaaaac cgctttgctg gttgctccgc tgtatgtgtt cgtcgccgcc tgggcgttat
    12181 ggctgcgggt cgctcagtac ggctggactg tcgaccgctt gcagggcgcg ctggcggtgc
    12241 tggtgttact ggtgtggtcg ctggggtatt ttgtcagtat cgtctggcgt aatgggcaaa
    12301 atccccttgt tcttcagggc aaagtgaacc ttgcggtttc gttattggtg ttggtgatac
    12361 tggtgcttct taattcgccg gtgctggaca gtatgcgcat tagcgtgaac agtcatatgg
    12421 cgcgttatca gagcggcaaa aacacgccag accaggtaac tatctacatg ctcgaacaga
    12481 gcggtcgcta tggacgtgcg gcgcttgagt cgctgaaaag cgatgccggg tttatgaaag
    12541 acccgaaacg cgcgcgggat ctgctgatgg cgttagatgg agagcaacat cttcaggaac
    12601 aggtatcgga aaaagtatta gccgaaaatg tgttaattgc tcctggttct gttaaaccgg
    12661 atgcgacatt ctggtcggcc ttaatccagg atcgctataa cgtgatgacc tgtattgaaa
    12721 aagacgcctg cgtcctggtc gagcaagatc tgaatagtga tggtcaggcg gagcggatcc
    12781 tgtttgcttt taatgatgac agagtcattg tctatggctt tgactcagac agaaaagaat
    12841 gggacgcgct tgatatgagt ttacttccga acgaaataac gaaagaaaaa ttactcacag
    12901 ctgcgaagga tggaaaactg gatctgacgg tagatggtga aacgctggaa ataaacttaa
    12961 gtaagtgaca actcttttaa cggatacgac gctgtatctg accaacaata atttgcgatt
    13021 tatttcacag gcctggtaag cgcagcacat caggccatta cgtcgtaaaa tccggcagag
    13081 ccctctctgc cggacatact cattaatgaa cttgcggatc tgccggagac gcgttgttgc
    13141 ggatttcagc aatatccatc gcattgaaca gatagtggtt accgcagtaa tcacaatgca
    13201 tgtcaatttc gccatcttcc gccaggatgc tatcgacttc ttcatcaggc agcgttttca
    13261 gcgcgtcggc gcaacgttca cgcgagcagg tgcatttgaa ctccacatcc tgcggatcgt
    13321 aaaccgtcac ctcttcttcg tgatacaaac gccacaacac ttcgtttgcc ggtaaggtca
    13381 gcagttcttc ggttttgatg gtttcggtta gcgtcgccag gtggtcaaag tcgtcctgct
    13441 gggcattttg cgcaggcatt acctgcaaca acataccgcc tgcagccggt ttgccgtcta
    13501 cgtcgccggt gcgaataaac aggcgcgtcg gcagctgttc agaacgcata aagtaatctt
    13561 ccaggcaggc cgccagggta tcaccttcca gaccaaccac gccctgatag cgttcgcctt
    13621 cgctcggggt aatggtgatc accacgtaac cattgccgac cagcgttttc aggtcggcat
    13681 tttctggaat ttcgccctgc acgcgcgcca caccgcgcat ttgctggtta ttgttaccgt
    13741 taataaccgc cagattcatc ggaccgtcgc cttgcagctg tacggtgata tcaccatcaa
    13801 acttcagcgt agcggttaac aggctggtcg caaccagcag ttctgccagc acgtttttaa
    13861 cgggctgcgg ataatcgtga ttctcaagga tctgttgcag ggtttccgaa acggttacca
    13921 gttcgccgcg cacggcaaag ttttcaaaca gatagcgatg taattggtca tgttgcggca
    13981 taatcatctc tcttgcaggt gacagttatt cactgtcgcc gtgtttaaat cgtaacaggt
    14041 cgcggcgctc ttttttgtcc gggcgtcggt ccgggtgcgg catggttaag gcattaagtt
    14101 tacgtgccag cgccattttt tcgcgtttct ctacactttc cgcagtctct tcatacagca
    14161 aggctgcctc gctggcgggg cgacgctgtt cagtaatcgc ctttacaatc accgtgcgtt
    14221 cgtcatttcc ctggcgcaga gtgagcgtgg cattcagctc gacgattttg ctcggcttgc
    14281 tgcgctgccc gttgtaatgc accttaccgc cttcaatcat ttcacgggcc agcgcgcggg
    14341 ttttataaaa acgggcagcc catagccatt tatccagtcg aacctcaaca gcaggttttt
    14401 ctttcatggc gtctccttca cattagcgag gggatcaggc ggcggtagtc attcagtgac
    14461 ggatggcgtt gatactgttt ctcggcaatc ccggaatcag gattagtcac gccgaggcag
    14521 taacgaatac caaattgcgc ggcagcatcg agaatcgctt cgctgtcatc aataaacagc
    14581 gttctttcag ctttcagacc cgtagcttcg gccaccgcat gccataaccg ctgatcctct
    14641 ttcggataac caaatgtgtg ggtggaaagt aataaatcaa ggtgtgcgtc cagaccggta
    14701 tgctcaagtt ttaccgccag gttgtgcgga tgcgcattgg tgagcaaaat tcgctgctta
    14761 ccgctggctt tcagtgcctc aagaaacgga atggtatctt cacgcagtac ggcacgcggt
    14821 cccatctcgg tggtcatcgc acagatatcc agacccagtt gctcactcca gtaatcaaga
    14881 cagtaccagt ttagcgtatg ctgtacgtcg tgatattgct ggcgcatata ttccatcgct
    14941 tcctgtggcg taaccccgtt tttcgcgccc catgtttcag gcaccagctt ttgccagaaa
    15001 tagttatcga aggcgaggtc gagcaacgtg ccgtccatat ccagcagaac ggtatctacg
    15061 tcctgccagg caatgttgat atgcatgagg gaaatctcca gagtgaagca atttgcgcga
    15121 cagggtagca taacctgccg cgcaaacgtg ttattcgata aggctttctg aaggggtgat
    15181 cagttgcggg ttcaggcagc tttcataata ggcctgaatc ttcatcatgc gagtgcgatg
    15241 acgctggtaa cgacgccagg cctgtacgcc attgtaaatg gcgctaccca gcatcgtcag
    15301 tagcagcaat gtggtgctga gatagcgcca caggccggaa cgatccggga ttggatgaag
    15361 gccaatatgc tgcgtaccat tggcgtcagt gaagattttt gtgacgatac cctcggcgtt
    15421 aaacggcgta tgcatcagca tttgtgccag tttctggaaa gcgttccact gttcttgcgg
    15481 cgggtagtcg taaagtgatg ccgaaggcca gggctgatca acaaaatcgc tgccttcgtc
    15541 gctgacaatc aggaatccgc cgggggccgg actgtttagt gattgtgccg ctcgcgccgt
    15601 ttcatgcgtg ataaacggcg cggtggaggt tgccaccagg ttatccagcg attccgcact
    15661 caccgggcgt aataacacat tcacgccatc gagcttcccg gcgttggcgc gttttaccag
    15721 cgcgtcccag tctttactgt tgccgagatt gaccagcgca tttttcagtc gcacacagtc
    15781 atctttggca gaacataaat ccgctgtctt cagtacaatg tcgccaaaat catcaagcaa
    15841 taccatgccg gatttttgaa ttgctgaacg taatgaggca ctgacgcgag atccatcttc
    15901 cggtttaggg tgcagctggc gattaactgc ttcagtcaat gccgtcgctt tgttgaccag
    15961 ttcagattct ggtaatggca atgagcgggc gtcgttccag atgatctgcg agcagtcaaa
    16021 cggtaaaaaa ggtgaattgg ttttcgcgct ccaggttccg gaagtgcgaa tattacacat
    16081 tcccgtaccg ctgatacgca atgtatcgcc tacccgcacg ccagcgtcag ccagttgttt
    16141 tacgctggtg gcttcaatgg tctgtgcgcc tttcatccat gagagagtga atttcagcgg
    16201 catatccagc gggatccaga ataacagcat aaacagcacc agcagcgagc ccgcagcgat
    16261 aatcgtactg cgcagccagt gctgtaacgg aaagtttttt acttcatcat gcagcgaaag
    16321 atatcgcccc tgacgcacta cgtggcggtc gagatagata tcaatatcgg tttgttgacc
    16381 gagatcctga gcaatgtatg gctgccagtg tgcgggatag accaggtcga taataccgag
    16441 tgaaatattg ttgatctgtt cctgatcgtt ttcgccaaac aatccccaac gacggggtgt
    16501 accgcgcaga caatgaattt cccgcaggga ggattttgcc gggggcgcga atagccccca
    16561 cagacctgcg cccagcagca gtaatgcgcc gcctgccagc cacggaacaa atacatccgg
    16621 ggtaatcagg cagaaaaaga acatcaggaa ggaggcgacg atcagcaacg cttcacgcag
    16681 cccgcgcgga cgactcagcg catattcttc atgcgtttct ttgcgaatat tgagtagctc
    16741 gatttgctca ctctcttccc cgcgaatcga cgcctgcgtg gacggaacag gttgcaaggc
    16801 atagccgcgc gtttcctgca tgtactcctg cagcgtatga ccgttgagtg aaataaccag
    16861 cggcagcgaa tcggtataaa tcagttcaac ggtattctca tcgttgatgt attgttccca
    16921 gaaagggggc aggtggactt ctaccgaatc gaggtagtaa cgccatttat tcggatcgtc
    16981 ggtagagatg ccgtaacgcg tgatagcgtg tgtcagcatc atgacgttgt tgctttcggc
    17041 attcagcgcc agagagatcg gtgccgcgct agctcccgtt gggccaggca cctgtaatac
    17101 ctgcgtcagg ctctcaagat aattttcaac ggcgctacgt tcttcaggtg tgagtttacg
    17161 cgtttgcgca tccgcgaagg cgttggtcca gggcagctga cgccgtctgg atcgcacttt
    17221 tatcagccat cccgcaagta gtgagcaggc cagcaaagca gctaaaaaaa tcacaatggt
    17281 gctcatgctt tccccatctt acttagtctg tcaggcgtgg tcagtcgcac cagacacaat
    17341 acatgattct gcggttggct atcggcaagc gtggagttta acttgagcat cattaagcac
    17401 atcacagtaa ccgctgatca tagcaaaccc tctgaaggcg ggatatcagc aaattcctgg
    17461 agttatttca acctattgac ttttaatttg atttgaggac aattatttat taagttacat
    17521 aaatgtaaat aacatgcaat tcacattgcc gaaagcgtgt ccgatatcgc acaataaccg
    17581 ctgatttcac agcatagatc ccacgatgag caaatcatta caaaaaccca ccattctgaa
    17641 tgttgaaact gtagcccatt cccgactgtt taccgtcgag agcgtggatc tggagttcag
    17701 caatggcgtg cggcgtgttt atgaacgaat gcgtccaacc aaccgggaag cagtgatgat
    17761 tgtgccgatt gtggacgatc acctgatcct gatccgcgaa tacgcagtgg gaactgaatc
    17821 ctacgaatta ggtttttcga aaggattaat tgatccgggt gaaagcgtct acgaagccgc
    17881 taaccgcgag ctaaaagaag aggttggatt tggtgcgaac gatctgactt ttttgaagaa
    17941 gctcagcatg gcaccgtctt acttttccag caaaatgaat atcgtggtcg cgcaagatct
    18001 ctacccggaa tcgctggaag gcgatgagcc agagccgcta ccacaggtgc gctggccgct
    18061 ggcgcatatg atggatttgc tggaagaccc tgacttcaac gaagcgcgta atgtcagcgc
    18121 gctgttcctc gtgcgcgaat ggttgaaagg gcaggggcga gtgtaaatgc gtgaagtttg
    18181 cctgatgcac aacgattatc aggcttatgg ggttcctgca atatattgat ttttcacgat
    18241 tttgttagcc ggataacgcg ttcacccgca tccggcataa acaaatgcac tttgtcagcc
    18301 aactgaaaag gcgccgaagc gcctttttaa tcagaacaat tcctgtgcct caccattatc
    18361 gataatggtt gttcccacct cgtgcaccgc ctgttgtgtc ggctgcgtac cttcgatgaa
    18421 atactcttcg cggctgttgc caccattagc taactgcccg gtgctgcgat cgatattcac
    18481 cgtcacaata cccggaggcg gcgtcagcgg ctgctccggc acaccttcaa gaacggcttt
    18541 catataagcg tcccatgcag gctgggcact cttggcaccg ccttcgtaac ctgagatctg
    18601 atctttaatc gctccggaag ccgttgtatg accgagatta cgacggtgat catcaaagcc
    18661 aatccagacc gaggtcacaa cgcccggacc gtaacccgag aaccacgcat ctttcgaact
    18721 gttagtggta ccggttttcc cgccgatatc gcgacgctgc aaatcacgac ctgcacgcca
    18781 gccggtaccc tgccagcctg gttcaccaaa gatattggtg ttcaaagcac tcttaatcag
    18841 gaatgccagc ggagtgttga tgacgtgcgg tgcgtactcc tgcgcgccag tcttcgccac
    18901 taacgcctga tttgcctgct ccagctgcgg cattggtaca gaaacattct gctgctcgcg
    18961 ggagatagcg acatcttcaa catcgttatt ttccagcacg ttcgatttct gcgtatcacc
    19021 gtaaatcacc ggaatatcgc attccgggca ggctactttc ggtttcgctt cgaaaatcac
    19081 gccgccctga tcgttttcaa ttttgctgat aaaccacggg tccaccagga agccgccgtt
    19141 cgccatgacc gcgtagccgc gcgccacctg cattggggtg aaggacgctg aacccagcgc
    19201 cagcgattcg gtgtggacaa tgttttgcgc cgggaagccg aagcgttgca gatattctgc
    19261 agcgtagtcg acgcccatcg cccgcattgc acgtaccatc accacgtttt tcgactgacc
    19321 cagcccctga cgtaagcgaa ttggaccagc atactgcggt ggtgagttct tcggctgcca
    19381 gtcagaaccg acacctgcat cccagcgaga aattggcaca tcgttcaaca tacttgccag
    19441 cgtcagacct ttatccatcg ccgcggtgta gaggaacggt ttgatgttgg aacccacctg
    19501 acgcagtgcc tgggtggcgc ggttaaactt gctctgattg aaatcaaagc caccgaccag
    19561 cgccataacg gcaccatttt gcggattgat cgacaccagc gccgagttca cttccggcac
    19621 ttgtgccagc caccatgcat cgccaacctg acgaacccag atttgctgac ccgtttgcag
    19681 aacatcggtc actttacgcg gcgtcggtcc ctgctgagta tccgaacggt aaggacgcgc
    19741 ccagcgaacg ccgtccatac tcaatgcgac agtcgacccg tccgccagca ttgccgtcgc
    19801 ttcctgagga ttggcgctgg tgactgcggc aggcagcagc ggaccatagg tcggcagcgc
    19861 cttcagcgta tcggtaatct tgttgttatc ccacgccgac tcgcccactt tccacagcac
    19921 atttgccggg ccgcgatagc cgtggcgcat gtcgtagtcc agcacgttat tacgtaccgc
    19981 ctgctgtgcg gcctgctgca ctttgcgggt gatggtggtg taaatgcgat aaccgtcttc
    20041 ataggcactt tcgccataac ggttatacat ctcctggcgc accatttcgc tcaggtacgg
    20101 cgcagagaaa gcaatctccg gcgcgtgata gttagcgtta atcgcctcag tgcgtgtctg
    20161 atcgaactgt tgttgggtga tatacccttc atccagcatc cgcgacagta cgacgttacg
    20221 ccgcgcgacg gcacgatcca ttgagtagag cggattaaag gtggaaggcg ctttcggcag
    20281 cccggctatc acagccattt cgttcagcgt cagttggtcg accgttttac cgaaatagac
    20341 ttgtgccgca gcaccgacac cataggcgcg gtaaccgagg taaatcttgt tcagataaag
    20401 ctcgaggatc tcgtctttcg tcagcaactg ttcaatgcga atcgcgagga agacttcctt
    20461 aatcttacgc atcagcgtgc gttctggact gaggaagaag tttctcgcca gctgctgggt
    20521 aatggtactc gccccttgtg acgcgtggcc ggagaacagc gccacacttg ccgcacggaa
    20581 gatccccacc gggtcaacgc cgtgatgctc gtagaagcgg ctgtcttccg tcgcgataaa
    20641 ggctttcacc atctccggcg ggatttgatc caacgtaacc ggaatacgac gtttctcacc
    20701 gtattgagca atcagctcgc catcggcgct gtaaatctgc atcggaattt gcaggcgaac
    20761 atcttttaat gtcgccacat ccggcagttg tggctcgatg tagcggtata ggccataaat
    20821 cgagcctgct cccagcagaa tgcaacagac tgcaaggatc aaaaaatact ttacgaactt
    20881 cactggaaat ttcccattta gtttcatttg ggcagtttat aaacaaacgt gcggtagtat
    20941 aaaggcaagc cagacgcatt gatatacccg tcagagtgac gggtgataag gagatcatca
    21001 caatggcatt taagatctgg caaattggtt tgcatttaca acagcaagaa gcagtagcgg
    21061 ttgcgatcgt gcggggcgca aaagaatgct ttttgcaacg ctggtggcgg ttgccgctgg
    21121 agaacgacat tatcaaagat gggcggattg ttgatgcgca gcggctggct aaaacgttgt
    21181 taccgtggag tcgcgaactg ccgcagcgtc atcacattat gttggcgttt cccgccagtc
    21241 gcacattaca gcggtcattt ccgcgcccgt cgatgtccct cggtgagcgg gagcaaacgg
    21301 cctggctgtc agggacgatg gcccgcgagc tggatatgga tccggactcc ctgcgtttcg
    21361 attacagcga agactcactc agtcccgcct ataacgtgac tgccgcgcaa agcaaagagc
    21421 tggcaacgct actcactctg gcagaaaggt tgcgtgttca tgtgagcgcg atcaccccgg
    21481 atgccagtgc attacagcga ttcctgcctt ttttaccttc tcatcagcaa tgtctggcct
    21541 ggcgtgataa cgaacagtgg ctgtgggcga cacgctgtcg ctgggggcgc aaactggcgg
    21601 tggggatgac tagcgcgaag gagctggcgg cagcgttatc cgttgatccc gaaagcgtcg
    21661 cgatatgtgg cgaaggcgga tttgatccct gggaggccgt ttctgttcgt cagccgccgc
    21721 taccgccgcc aggtggagac tttgccatcg cgctggggct ggcgcttggg aaggcgtact
    21781 gatgaacccg ccaattaatt ttttgccctg gcgacagcaa cgccggaccg cttttcttcg
    21841 tttctggttg ctgatgttcg ttgcgcctct gctgctggcc gtcgggataa cgctaatact
    21901 gcgtctgaca gacaacgccg aagcacgcgt aaacaccgtt ttgcttcagg cggaacaaca
    21961 actcgcccgc agcttacaga taacgaaacc acgtttgctg gagcgccaac aaattcgtga
    22021 gcagcgtttg caacggcagc gccagcgaca atttacccgc gactggcaat ctgcgctgga
    22081 agcactggcg gcgcttttac ccgagcacgc ctggctgaca acgataagct ggcagcaggg
    22141 aacgctggag atcaaggggc tgacaacaag cattaccgcg ttaaacgcac tagaaacgtc
    22201 actccgccag gatgcgtctt ttcatctcaa tcagcgggga gccacgcagc aggatgcgca
    22261 gggacgctgg caatttgagt atcagttaac aaggaaggtt agcgatgaac atgttctttg
    22321 actggtggtt cgccacatca ccccgcctcc gccagttttg ctgggcattc tggttgctga
    22381 tgttagttac gctcattttt ctgtcatcga cacaccatga agagcgcgac gcattaattc
    22441 gactacgggc aagtcatcac cagcagtggg ccacactgta tcgcctggtt gacaccactc
    22501 ccttcagcga agaaaaaacg ctgccctttt cgccactgga ttttcagttg cccggcgcgc
    22561 aactggtttc ctggcatcca tccgcgcagg gaggcgagtt ggcgttgaaa acgctgtggg
    22621 aagcagtgcc gtcggcattt acacggctgg cagagcgcaa cgtcagcgtg agccgttttt
    22681 cattaagcgt ggaaggtgat gatcttttgt tcacgctaca actggagacg ccgcatgagg
    22741 gttaaacgct ggttgttggc aggtattgta ttgtgccttt taaccggtat gcgtgaccct
    22801 tttaaaccgc cggaagatct atgccggatt agcgaactta gccagtggcg ctatcagggg
    22861 atggtagggc gaggcgagcg catcatcggt gtaataaaag acgggcaaaa gaaatggcga
    22921 cgggtgcagc aaaacgatgt gctggaaaac ggctggacaa ttttacagct gacgccagac
    22981 gtactaacgc tgggtaccgg gacaaactgc gaaccgccac aatggttgtg gcaacggcaa
    23041 ggagatacaa atgaagcaat ggatagccgc actactgttg atgctgatac ccggcgtaca
    23101 ggcggcaaag ccgcaaaaag tgacgctgat ggtggatgac gttccggtag ctcaggtgtt
    23161 gcaggcgctg gctgaacagg agaagttgaa cctggtcgtg tcgccagacg tcagcggtac
    23221 ggtgtcgtta catctaacag atgttccctg gaagcaggca ctacaaactg tagtgaaaag
    23281 cgccggactg ataacgcggc aggaaggcaa cattctctca gtgcattcca ttgcctggca
    23341 gaataacaat atcgcccgcc aggaggcgga gcaggcgcgg gcgcaggcaa atctgccgct
    23401 ggaaaatcgc agtataaccc tgcaatacgc cgacgcggga gaactggcga aagcggggga
    23461 gaagctactg agtgccaaag ggagtatgac cgtcgataaa cgcaccaatc gccttttgct
    23521 acgagataac aaaacggcgt taagcgcgct tgaacagtgg gtagcgcaaa tggatctgcc
    23581 ggtcgggcag gttgagctgt cggcgcatat tgtcaccatt aatgaaaaaa gtttgcgtga
    23641 gttaggcgtg aaatggacgc tggccgatgc gcaacacgct ggtggcgttg ggcaagtcac
    23701 cacgcttggt agcgacctct ccgtagcgac ggcgacaacg catgtcggtt ttaacattgg
    23761 gcgcatcaac ggacgcttgc tggatcttga gctttccgcg ctcgaacaaa aacagcagct
    23821 ggatattatc gccagtccgc gtctgctggc ctcacatctt cagcctgcca gcattaaaca
    23881 ggggagcgaa attccatatc aggtttccag cggggaaagt ggcgcgacgt cggtggaatt
    23941 taaagaggcc gtcctgggga tggaagttac gcccacagtg ttacaaaaag gtcgcatccg
    24001 gctgaaatta cacatcagcc agaacgttcc ggggcaggtg ctacagcagg ccgatggcga
    24061 agtgctggcg attgataagc aggagatcga aacgcaggtc gaggtcaaaa gcggagaaac
    24121 gttggcgctg ggcggcattt ttacccgtaa aaataaatcg ggtcaggata gcgtaccgtt
    24181 gcttggcgac attccctggt tcgggcaatt atttcgtcat gacggaaaag aagatgaacg
    24241 acgcgagtta gtggtgttta tcacgccacg actggtttcc agtgagtaaa cagccgtaaa
    24301 agcggtaatg tttttacgct gaacgtgttt catctatttg acgcgcgcag gtatttagca
    24361 tacaaggagt accgatttga gagttggtgc tcttcgctgc ctgcgttcca tgatgatgat
    24421 ttatcattca ggcggcattt tgctgtcttt tttacgctaa tcttacccgg tgatttatcg
    24481 ccagagcggt ggtagcaagg cagcgcgctt gcagcgacca gatatgcaga gggatgggtg
    24541 atttattcag ttgccaaacc cgctggagta ttgagataat tttcagtctg actctcgcaa
    24601 tatcttatga ggtttcagtt catgtcctgc ggcgctctct gagcgaagcg ggtttatcat
    24661 taacgaatag tcttagtagt accgaaaaaa tggcagagaa acgcaatatc tttctggttg
    24721 ggcctatggg tgccggaaaa agcactattg ggcgccagtt agctcaacaa ctcaatatgg
    24781 aattttacga ttccgatcaa gagattgaga aacgaaccgg agctgatgtg ggctgggttt
    24841 tcgatttaga aggcgaagaa ggcttccgcg atcgcgaaga aaaggtcatc aatgagttga
    24901 ccgagaaaca gggtattgtg ctggctactg gcggtggctc tgtgaaatcc cgtgaaacgc
    24961 gtaaccgtct ttccgctcgt ggcgttgtcg tttatcttga aacgaccatc gaaaagcaac
    25021 ttgcacgcac gcagcgtgat aaaaaacgcc cgttgctgca cgttgaaaca ccgccacgtg
    25081 aagttctgga agcgttggcc aatgaacgca atccgctgta tgaagagatt gccgacgtga
    25141 ccattcgtac tgatgatcaa agcgctaaag tggttgcaaa ccagattatt cacatgctgg
    25201 aaagcaacta attctggctt tatatacact cgtctgcggg tacagtaatt aaggtggatg
    25261 tcgcgttatg gagaggattg ttgttactct cggggaacgt agttacccaa ttaccatcgc
    25321 atctggtttg tttaatgaac cagcttcatt cttaccgctg aaatcgggcg agcaggtcat
    25381 gttggtcacc aacgaaaccc tggctcctct gtatctcgat aaggtccgcg gcgtacttga
    25441 acaggcgggt gttaatgtcg atagcgttat cctccctgac ggcgagcagt ataaaagcct
    25501 ggctgtactc gataccgtct ttacggcgtt gttacaaaag ccgcatggtc gcgatactac
    25561 gctggtggcg cttggcggcg gcgtagtggg cgatctgacc ggcttcgcgg cggcgagtta
    25621 tcagcgcggt gttcgtttca ttcaagtccc gacgacgtta ctgtcgcagg tcgattcctc
    25681 cgttggcggc aaaactgcgg tcaaccatcc cctcggtaaa aacatgattg gcgcgttcta
    25741 ccagcctgct tcagtggtgg tggatctcga ctgtctgaaa acgcttcccc cgcgtgagtt
    25801 agcgtcgggg ctggcagaag tcatcaaata cggcattatt cttgacggtg cgttttttaa
    25861 ctggctggaa gagaatctgg atgcgttgtt gcgtctggac ggtccggcaa tggcgtactg
    25921 tattcgccgt tgttgtgaac tgaaggcaga agttgtcgcc gccgacgagc gcgaaaccgg
    25981 gttacgtgct ttactgaatc tgggacacac ctttggtcat gccattgaag ctgaaatggg
    26041 gtatggcaat tggttacatg gtgaagcggt cgctgcgggt atggtgatgg cggcgcggac
    26101 gtcggaacgt ctcgggcagt ttagttctgc cgaaacgcag cgtattataa ccctgctcaa
    26161 gcgggctggg ttaccggtca atgggccgcg cgaaatgtcc gcgcaggcgt atttaccgca
    26221 tatgctgcgt gacaagaaag tccttgcggg agagatacgc ttaattcttc cgttggcaat
    26281 tggtaagagt gaagttcgca gcggcgtttc gcacgagctt gttcttaacg ccattgccga
    26341 ttgtcaatca gcgtaacaac aagaaaggtc aggccgctta tcaagcggtc tattagcttc
    26401 aggttaattg caacgtggta agcattaacc ttttagtggg gtgttaaatg gatgaattca
    26461 aaccagaaga cgagctgaaa cccgatccca gcgatcgtcg tactggtcgt tctcgtcaat
    26521 cttctgaacg ttctgagcgt actgaacgtg gcgaaccgca gatcaatttt gatgatattg
    26581 aacttgatga cactgacgat cgccgtccga ctcgtgcgca aaaagagcgc aatgaggaac
    26641 cggaaatcga agaagaaatt gacgaatccg aagatgaaac cgtggatgaa gagcgcgtag
    26701 agcgtcgtcc gcgtaagcgc aaaaaagcag ccagtaaacc cgcttctcgt cagtatatga
    26761 tgatgggcgt cggcattctg gttctactgc tgttgatcat cggtatcggt tctgcgctaa
    26821 aagccccctc gacctcttcc agcgatcaaa ccgcgtctgg cgagaagagt attgatcttg
    26881 caggcaatgc gaccgatcag gcgaatgggg tgcagccagc gccgggaacc acgtctgcgg
    26941 aaaatactca gcaggatgtt tctctgccac cgatctcttc tacgccgact caagggcaaa
    27001 ccccggcggc aacggatggt caacaacgtg ttgaagtgca gggtgacctg aacaatgcgc
    27061 tgacccagcc acaaaatcag caacagttga acaatgtggc ggtcaattcc acattgccga
    27121 ccgaacccgc gactgtcgcg cctgttcgca atggcaatgc atcgcgtgac acggcgaaaa
    27181 cgcaaaccgc tgaacgtccg tccactacgc gcccagctcg tcagcaggcg gtgattgaac
    27241 cgaaaaaacc gcaagcaacc gtgaaaacgg agccgaagcc ggtagcacag acgccgaagc
    27301 gtactgaacc agctgcccct gtggcgagca cgaaggcacc gactgcgact tctacgccag
    27361 caccaaaaga gacggcgact acggctccag tacagacggc atccccggcg caaaccacgg
    27421 caacaccagc cgctggaggg aagaccgcag gtaatgttgg ttcgttgaaa tcggcaccgt
    27481 ccagccatta cactctgcag ctgagcagtt cctctaacta cgacaacctg aacggttggg
    27541 cgaagaaaga gaatctgaaa aactacgttg tctatgaaac ggcgcgtaat ggtcagccgt
    27601 ggtatgtcct ggtttctggc gtgtacgctt cgaaagaaga ggcgaaaaaa gcggtatcta
    27661 cattgccagc agatgttcag gccaaaaacc cgtgggcgaa accgctgcgt caggtacagg
    27721 ccgatctgaa gtaatcaagg ttatctcccg caatggttta tcgttgcggg agctgcctga
    27781 agcgctggat gctgtcggag ctttctccac agccggagaa ggtgtaatta gttagtcagc
    27841 atgaagaaaa atcgcgcttt tttgaagtgg gcagggggca agtatcccct gcttgatgat
    27901 attaaacggc atttgcccaa gggcgaatgt ctggttgagc cttttgtagg tgccgggtcg
    27961 gtgtttctca acaccgactt ttctcgttat atccttgccg atatcaatag cgacctgatc
    28021 agtctctata acattgtgaa gatgcgtact gatgagtacg tacaggccgc acgcgagctg
    28081 tttgttcccg aaacaaattg cgccgaggtt tactatcagt tccgcgaaga gttcaacaaa
    28141 agccaggatc cgttccgtcg ggcggtactg tttttatatt tgaaccgcta cggttacaac
    28201 ggcctgtgtc gttacaatct gcgcggtgag tttaacgtgc cgtttggccg ctacaaaaaa
    28261 acctatttcc cggaagcaga gttgtatcac ttcgctgaaa aagcgcagaa tgcctttttc
    28321 tattgtgagt cttacgccga tagcatggcg cgcgcagatg atgcatccgt cgtctattgc
    28381 gatccgcctt atgcaccgct gtctgcgacc gccaacttta cggcgtatca cacaaacagt
    28441 tttacgcttg aacaacaagc gcatctggcg gagatcgccg aaggtctggt tgagcgccat
    28501 attccagtgc tgatctccaa tcacgatacg atgttaacgc gtgagtggta tcagcgcgca
    28561 aaattgcatg tcgtcaaagt tcgacgcagt ataagcagca acggcggcac acgtaaaaag
    28621 gtggacgaac tgctggcttt gtacaaacca ggagtcgttt cacccgcgaa aaaataattc
    28681 tcaaggagaa gcggatgaaa cagtatttga ttgccccctc aattctgtcg gctgattttg
    28741 cccgcctggg tgaagatacc gcaaaagccc tggcagctgg cgctgatgtc gtgcattttg
    28801 acgtcatgga taaccactat gttcccaatc tgacgattgg gccaatggtg ctgaaatcct
    28861 tgcgtaacta tggcattacc gcccctatcg acgtacatct gatggtgaaa cccgtcgatc
    28921 gcattgtgcc tgatttcgct gccgctggtg ccagcatcat tacctttcat ccagaagcct
    28981 ccgagcatgt tgaccgcacg ctgcaactga ttaaagaaaa tggctgtaaa gcgggtctgg
    29041 tatttaaccc ggcgacacct ctgagctatc tggattacgt gatggataag ctggatgtga
    29101 tcctgctgat gtccgtcaat cctggtttcg gcggtcagtc tttcattcct caaacactgg
    29161 ataaactgcg cgaagtacgt cgccgtatcg acgagtctgg ctttgacatt cgtctggaag
    29221 tggacggtgg cgtgaaggtg aacaacattg gcgaaatcgc tgcggcgggc gcggatatgt
    29281 tcgtcgccgg ttcggcaatc ttcgaccagc cagactacaa aaaagtcatt gatgaaatgc
    29341 gcagtgaact ggcaaaggta agtcatgaat aagtttgaag atattcgcgg cgtcgctttt
    29401 gatcttgatg gtacgctggt cgacagtgct cccggtcttg ctgctgcggt agatatggcg
    29461 ctgtatgcgc tggagttgcc catcgcaggt gaagaacgcg ttattacctg gattggtaac
    29521 ggcgcagatg ttctgatgga gcgcgcattg acctgggcgc gtcaggaacg tgcgactcag
    29581 cgtaaaacaa tgggtaaacc gcccgttgat gacgacattc cggcagaaga acaggtacgt
    29641 attctgcgta aactgttcga tcgctactat ggcgaggttg ccgaagaggg gacgtttttg
    29701 ttcccgcacg ttgccgatac gttgggcgcg ttgcaggcta aaggcctgcc gctaggcctg
    29761 gtcaccaaca aaccgacgcc gttcgtcgca ccgctgctcg aagccttaga tattgccaaa
    29821 tactttagcg tggttatcgg cggcgatgat gtgcaaaaca aaaaaccgca tccggacccg
    29881 ctgttactgg tggctgagcg gatgggaatt gccccacaac agatgctgtt tgtcggcgac
    29941 tcacgcaatg atattcaggc ggcaaaagcg gcaggttgcc catcagttgg cttaacctac
    30001 ggatataact acggcgaggc tatcgatctc agccagcctg atgtaattta tcagtctata
    30061 aatgaccttc tgcccgcatt agggcttccg catagcgaaa atcaggaatc gaaaaatgac
    30121 taagcccatc gtttttagtg gcgcacagcc ctcaggtgaa ttgaccattg gtaactacat
    30181 gggtgcgctg cgtcagtggg taaacatgca ggatgactac cattgcattt actgtatcgt
    30241 tgaccaacac gcgatcaccg tgcgccagga tgcacagaag ctgcgtaaag cgacgctgga
    30301 tacgctggcc ttgtatctgg cttgtggtat cgatcctgag aaaagcacca tttttgttca
    30361 gtcccacgta ccagaacatg cgcagttagg ctgggcactg aactgttata cctacttcgg
    30421 cgaactgagt cgcatgacgc agtttaaaga taaatctgcg cgttatgccg agaacatcaa
    30481 cgctggtctg tttgactatc cggtgctgat ggctgcggac atcctgctgt atcaaactaa
    30541 tctggtaccg gtgggtgaag accagaaaca gcacctggaa ctgagtcgcg atatcgccca
    30601 gcgtttcaac gcgctgtatg gcgagatctt taaggtgccg gagccgttta ttccgaaatc
    30661 tggcgcgcgc gtaatgtcgc tgctggagcc gaccaagaag atgtccaagt ctgacgataa
    30721 ccgcaataac gttatcggcc tgctggaaga tccgaaatcg gtagtgaaga aaatcaaacg
    30781 cgcggtcact gactccgacg agccgccggt agttcgctac gatgtgcaga acaaagcggg
    30841 cgtttccaac ctgctggata tcctttctgc ggtaacgggc cagagcatcc cagaactgga
    30901 aaaacagttc gaaggcaaga tgtatggtca tctgaaaggt gaagtggctg atgccgtttc
    30961 cggtatgctg actgaattgc aggaacgcta tcaccgtttc cgcaacgatg aagccttcct
    31021 gcaacaggtg atgaaagacg gcgcagaaaa agccagtgta cacgcttccc gtacgctgaa
    31081 agcggtgtac gaagcgattg gttttgtggc gaagccgtaa tttaacatta atgccaaaaa
    31141 ccggggaaac ccggtttttt tatcctcatt tgcaataatc cgaaaaaatg tgaagcgcct
    31201 cgccgtttcc acatctcgtc gttgc
//
LOCUS       DAZPDU010000032        31117 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZPDU010000032 DAZPDU010000000
VERSION     DAZPDU010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31117
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.032"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            19..714
                     /gene="araD"
                     /locus_tag="ACHURS_001348"
     CDS             19..714
                     /gene="araD"
                     /locus_tag="ACHURS_001348"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFR1237126.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            789..3140
                     /gene="polB"
                     /locus_tag="ACHURS_001349"
     CDS             789..3140
                     /gene="polB"
                     /locus_tag="ACHURS_001349"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFR1237127.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGKQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHTSPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            3304..6210
                     /gene="rapA"
                     /locus_tag="ACHURS_001350"
     CDS             3304..6210
                     /gene="rapA"
                     /locus_tag="ACHURS_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFR1237128.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPVQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            6222..6881
                     /gene="rluA"
                     /locus_tag="ACHURS_001351"
     CDS             6222..6881
                     /gene="rluA"
                     /locus_tag="ACHURS_001351"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFR1237129.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(6998..7813)
                     /gene="djlA"
                     /locus_tag="ACHURS_001352"
     CDS             complement(6998..7813)
                     /gene="djlA"
                     /locus_tag="ACHURS_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFR1237130.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            8068..10422
                     /gene="lptD"
                     /locus_tag="ACHURS_001353"
     CDS             8068..10422
                     /gene="lptD"
                     /locus_tag="ACHURS_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFR1237131.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWQHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            10475..11761
                     /gene="surA"
                     /locus_tag="ACHURS_001354"
     CDS             10475..11761
                     /gene="surA"
                     /locus_tag="ACHURS_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFR1237132.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            11761..12750
                     /gene="pdxA"
                     /locus_tag="ACHURS_001355"
     CDS             11761..12750
                     /gene="pdxA"
                     /locus_tag="ACHURS_001355"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFR1237133.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLNELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            12747..13568
                     /gene="rsmA"
                     /locus_tag="ACHURS_001356"
     CDS             12747..13568
                     /gene="rsmA"
                     /locus_tag="ACHURS_001356"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFR1237134.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            13571..13948
                     /gene="apaG"
                     /locus_tag="ACHURS_001357"
     CDS             13571..13948
                     /gene="apaG"
                     /locus_tag="ACHURS_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFR1237135.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            13955..14797
                     /gene="apaH"
                     /locus_tag="ACHURS_001358"
     CDS             13955..14797
                     /gene="apaH"
                     /locus_tag="ACHURS_001358"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFR1237136.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(14875..15354)
                     /gene="folA"
                     /locus_tag="ACHURS_001359"
     CDS             complement(14875..15354)
                     /gene="folA"
                     /locus_tag="ACHURS_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFR1237137.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(15546..17408)
                     /gene="kefC"
                     /locus_tag="ACHURS_001360"
     CDS             complement(15546..17408)
                     /gene="kefC"
                     /locus_tag="ACHURS_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFR1237138.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLHWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGTAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(17401..17931)
                     /gene="kefF"
                     /locus_tag="ACHURS_001361"
     CDS             complement(17401..17931)
                     /gene="kefF"
                     /locus_tag="ACHURS_001361"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFR1237139.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGKALHGKYLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(18039..19370)
                     /locus_tag="ACHURS_001362"
                     /pseudo
     CDS             complement(18039..19370)
                     /locus_tag="ACHURS_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(19429..19716)
                     /gene="fixX"
                     /locus_tag="ACHURS_001363"
     CDS             complement(19429..19716)
                     /gene="fixX"
                     /locus_tag="ACHURS_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFR1237140.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(19713..20999)
                     /locus_tag="ACHURS_001364"
     CDS             complement(19713..20999)
                     /locus_tag="ACHURS_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR1237141.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMNYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(21050..21991)
                     /locus_tag="ACHURS_001365"
     CDS             complement(21050..21991)
                     /locus_tag="ACHURS_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFR1237142.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(22006..22776)
                     /locus_tag="ACHURS_001366"
     CDS             complement(22006..22776)
                     /locus_tag="ACHURS_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFR1237143.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            23249..24763
                     /gene="caiT"
                     /locus_tag="ACHURS_001367"
     CDS             23249..24763
                     /gene="caiT"
                     /locus_tag="ACHURS_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFR1237144.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            24794..25936
                     /gene="caiA"
                     /locus_tag="ACHURS_001368"
     CDS             24794..25936
                     /gene="caiA"
                     /locus_tag="ACHURS_001368"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFR1237145.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            26065..27282
                     /gene="caiB"
                     /locus_tag="ACHURS_001369"
                     /pseudo
     CDS             26065..27282
                     /gene="caiB"
                     /locus_tag="ACHURS_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
     gene            27356..28909
                     /gene="caiC"
                     /locus_tag="ACHURS_001370"
     CDS             27356..28909
                     /gene="caiC"
                     /locus_tag="ACHURS_001370"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFR1237146.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTTNLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATVTECIPMMIRTL
                     MAQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            29018..29803
                     /gene="caiD"
                     /locus_tag="ACHURS_001371"
     CDS             29018..29803
                     /gene="caiD"
                     /locus_tag="ACHURS_001371"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFR1237147.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            29809..30399
                     /gene="caiE"
                     /locus_tag="ACHURS_001372"
     CDS             29809..30399
                     /gene="caiE"
                     /locus_tag="ACHURS_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFR1237148.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(30608..31003)
                     /gene="caiF"
                     /locus_tag="ACHURS_001373"
     CDS             complement(30608..31003)
                     /gene="caiF"
                     /locus_tag="ACHURS_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFR1237149.1"
                     /translation="MCEGYVEKPPYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCAVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
BASE COUNT         7541 a         8375 c         8150 g         7051 t
ORIGIN      
        1 gacctctcag gagtcaacat gttagaagat ctcaaacgcc aggtattaga agccaacctg
       61 gcgctgccaa aacacaacct ggtcacgctc acatggggca acgtcagcgc cgttgatcgc
      121 gagcgcggcg tctttgtgat caaaccttcc ggcgtcgatt acagcgtcat gaccgctgac
      181 gatatggtcg tggttagcat cgaaaccggt gaagtggttg aaggtacgaa aaagccctcc
      241 tccgacacgc caactcaccg gctgctctat caggcattcc cctccattgg cggcattgtg
      301 catacgcact cgcgccacgc caccatctgg gcgcagtcgg gtcagtcgat tccagcaacc
      361 ggcaccaccc acgccgacta tttctacggc accattccct gcacccgcaa aatgaccgac
      421 gcagaaatca acggcgaata tgagtgggaa accggtaacg tcatcgtaga aacctttgaa
      481 aaacagggta tcgatgcagc gcaaatgccc ggcgttctgg tccattccca cggcccgttt
      541 gcatggggca aaaatgccga agatgcggtg cataacgcca tcgtgctgga agaggtcgct
      601 tatatgggga tattctgccg tcagttagcg ccgcagttac cggatatgca gcaaacgctg
      661 ctggataaac actatctgcg taagcatggc gcgaaggcat attacgggca gtaatgactg
      721 tataaaacca cagccaatca aacgaaacca ggctatactc aagcctggtt ttttgatgga
      781 ttttcagcgt ggcgcaggca ggttttatct taacccgaca ctggcgggac accccgcaag
      841 ggacagaagt ctccttctgg ctggcgacgg acaacgggcc gttgcaggtt acgcttgcac
      901 cgcaagagtc cgtggcgttt attcccgccg atcaggttcc ccgcgctcag catattttgc
      961 agggtaaaca aggctttcgc ctgacaccgc tggcgttaaa ggattttcac cgccagccgg
     1021 tgtatggcct ttactgtcgc gcccatcgcc aattgatgaa ttacgaaaag cgcctgcgtg
     1081 aaggtggcgt taccgtctac gaggccgatg tgcgcccgcc agaacgctat ctgatggagc
     1141 ggtttatcac ctcaccggtg tgggtcgagg gtgatatgca caatggcact atcgttaatg
     1201 cccgtctgaa accgcatccc gactatcgtc cgccgctcaa gtgggtttct atagatattg
     1261 aaaccacccg ccacggtgag ctgtactgca tcggcctgga aggctgcggg cagcgcatcg
     1321 tttatatgct ggggccggag aatggcgacg cctcctcgct tgatttcgaa ctggaatacg
     1381 tcgccagccg cccgcagttg ctggaaaaac tcaacgcctg gtttgccaac tacgatcctg
     1441 atgtgatcat cggttggaac gtggtgcagt tcgatctgcg aatgctgcaa aaacatgccg
     1501 agcgttaccg tcttccgctg cgtcttgggc gcgataatag cgagctggag tggcgcgagc
     1561 acggctttaa aaacggcgtc ttttttgccc aggctaaagg tcggctaatt atcgacggta
     1621 tcgaggcgct gaaatccgcg ttctggaatt tctcttcatt ctcgctggaa actgtcgctc
     1681 aggagctatt aggcgaagga aaatctatcg ataacccgtg ggatcgaatg gacgaaattg
     1741 accgccgttt cgccgaagat aaacctgcgc tggcaactta taacctgaaa gattgcgagc
     1801 tggtgacgca gatcttccac aaaactgaaa tcatgccatt tttactcgaa cgagcaacgg
     1861 tgaacggcct gccggtggat cggcacggcg gttcggtggc agcgtttggt catctctatt
     1921 ttccgcgaat gcatcgcgct ggttatgtcg cgcctaatct cggcgaagtg ccgccgcaca
     1981 ccagccctgg cggctacgtg atggattcac ggccagggct ttatgattca gtgctggtgc
     2041 tggactataa aagcctgtac ccgtcgatca tccgcacctt tctgattgat cccgtcgggc
     2101 tggttgaagg catggcgcag cctgatccag agcacagtac cgaaggtttt ctcgatgcct
     2161 ggttctcacg ggagaaacat tgcctgccgg agattgtgac taacatctgg cacgggcgcg
     2221 atgaagccaa acgtcagggt aataaaccac tttcgcaggc gctgaagatc attatgaatg
     2281 ccttttatgg cgtgcttggc accaccgcct gccgcttttt cgatccgcgc ctggcatcgt
     2341 cgatcaccat gcgtggtcat cagatcatgc ggcaaaccaa agcattgatt gaagcgcagg
     2401 gctacgacgt tatctacgga gataccgact cgacttttgt ctggctaaaa ggtgcacatt
     2461 cggaagaaga ggcggcgaaa atcggtcgtg cactggtgca gcacgttaac gcctggtggg
     2521 cggaaacgct gcaaaaacaa cggctgacca gcgcattaga actggagtat gaaactcatt
     2581 tctgccgttt tcttatgcca accattcgcg gagctgatac cggcagtaaa aagcgctacg
     2641 ccgggctgat tcaggagggc gataaacagc ggatggtgtt taaagggctg gaaaccgtgc
     2701 gcaccgactg gacgccgctg gcccagcagt ttcagcagga gctatacctg cgcatcttcc
     2761 gcaacgagcc atatcaggaa tatgtacgcg aaaccatcga caaactgatg gcgggtgaac
     2821 tggatgcgcg actggtttac cgtaaacgcc ttcgccgtcc gctgagcgag tatcagcgta
     2881 atgtgccgcc tcatgtacgc gccgctcgcc ttgccgatga agaaaaccaa aagcgtggtc
     2941 gccccttgca atatcagaat cgcggcacca ttaagtacgt atggaccacc aacggcccgg
     3001 agccgctgga ctaccaacgt tcaccactgg attacgaaca ctatctgacc cgccagctac
     3061 aacccgtggc ggagggaata ctccctttta ttgaggataa ttttgctaca cttatgaccg
     3121 ggcaacttgg gctattttga gcaaaaaaag agttcgccag ataccatttt gatgcgtgac
     3181 gaatgctttg ccatccagta ccatagcgcc ctttccattc ctggacctga ataacaccac
     3241 taccccataa gcacggtagc gggtggttat tgcctgcaat taaagatata gagccgaaca
     3301 catatgcctt ttacacttgg tcaacgctgg atcagcgata cagaaagcga attgggactt
     3361 ggaaccgttg tcgcggtgga tgcgcgaact gtcactttac ttttcccatc tactggtgaa
     3421 aaccgtctgt acgcacgcag tgattccccc gtgacccgcg tgatgttcaa ccctggtgat
     3481 actattacca gccatgacgg ctggcagatg caagtcgaag aagtaaaaga agaaaatggc
     3541 ttgctgacct atatcggtac tcgcctggat actgaagagt ccggcgtagc cctgcgtgaa
     3601 gttttccttg atagcaaact ggtgttcagc aaaccgcagg accgtctgtt tgccgggcag
     3661 attgaccgta tggaccgctt tgcgctgcgt tatcgcgcgc gtaagtattc cagcgaacag
     3721 ttccgtatgc cgtacagcgg cctgcgcggt cagcgtacca gcctgatccc gcatcagctc
     3781 aacatcgctc atgatgttgg tcgccgccac gcgccgcgcg tcctgctggc tgacgaagtg
     3841 ggtttaggga aaaccattga agccgggatg atcctgcatc agcaactgct ctctggcgct
     3901 gctgaacgtg tgctaattat cgtcccggaa accttacagc atcagtggct ggtagaaatg
     3961 ctgcgccgtt tcaacctgcg ctttgcgcta tttgatgatg agcgttatgc cgaagctcag
     4021 cacgatgctt acaacccgtt cgacaccgaa cagctggtga tttgctcgct ggattttgcc
     4081 cgtcgtagca aacagcgcct ggaacatctc tgtgaagccg aatgggacct gctggtggtc
     4141 gatgaagcgc atcacctggt gtggagcgaa gatgcgccaa gccgtgaata tcaggccatt
     4201 gaacaactgg cagagcacgt gccgggcgtt ctgctgctga ccgcgacccc ggaacagctg
     4261 gggatggaaa gccacttcgc ccgtctgcgt ctgctggacc cgaaccgttt ccacgatttt
     4321 gcgcagttcg ttgaagagca gaaaaattat cgtccggttg cggacgccgt tgccatgctg
     4381 ctggcaggta acaaactgag caatgacgaa ctgaacatgc tcggcgagat gatcggcgag
     4441 caggatatcg agccgctgtt gcaggcagca aacagcgaca gcgaagatgc ccagagcgcc
     4501 cgtcaggagc tggtttcgat gctgatggat cgccacggca ccagccgcgt gctgttccgt
     4561 aacacgcgta acggtgtgaa aggattcccg aaacgcgagc tgcacaccat taagctgccg
     4621 ctgccgacgc agtatcagac ggctattaaa gtctccggca ttatgggcgc acgtaaaagt
     4681 gcggaagatc gtgctcgcga tatgctctac ccggagcgta tttatcagga atttgaaggt
     4741 gataacgcca cctggtggaa cttcgatccg cgcgttgagt ggctgatggg ctacctgacc
     4801 agccatcgct ctcagaaagt gctggtgatc tgcgccaaag ctgccactgc gctgcaactg
     4861 gagcaggtac tgcgcgaacg tgaaggtatt cgcgctgcgg tgttccacga aggtatgtcg
     4921 attatcgaac gtgaccgcgc tgccgcctgg tttgccgaag aagacaccgg cgcacaggta
     4981 ctgctgtgct cagaaatcgg ttctgaagga cgtaacttcc agttcgccag ccacatggtg
     5041 atgtttgacc tgccattcaa cccggatcta ctggagcagc gtattggtcg tctggatcgt
     5101 atcggccagg cgcacgatat tcagatccat gtgccttatc tggagaaaac cgctcagtcg
     5161 gtgctggtgc gctggtatca cgaaggtctg gatgcatttg agcacacctg cccgaccgga
     5221 cgcactattt acgatagcgt atacaacgat ctgattaact atctggcttc accggttcaa
     5281 accgaaggct ttgacgatct gatcaaaaac tgccgcgagc aacatgaagc gctgaaagca
     5341 cagctggaac agggtcgtga ccgcctgctg gaaatccact ccaacggtgg cgaaaaagcc
     5401 caggcactgg cagaaagcat tgaagagcag gatgacgata ccaacctgat cgccttcgcc
     5461 atgaacctgt tcgatattat cggtatcaat caggacgatc gcggcgacaa catgatcgtg
     5521 ctgacgccgt ccgatcatat gctggtgccg gacttccctg gcctgtcgga agatggcatc
     5581 accatcacct ttgatcgtga agtggcgctg gcgcgtgaag atgcacagtt tattacctgg
     5641 gagcatccgc tgatccgcaa cggtctggat ctgatccttt ctggcgatac tggtagcagc
     5701 acgatttcac tattgaaaaa caaagcgttg ccggtaggta cgctgttggt ggaactgatt
     5761 tatgtcgttg aagcccaggc tccgaagcag ttgcagctca accgcttcct gccaccgacg
     5821 ccggtacgta tgctgctgga taaaaacggc aacaacctgg cggcgcaggt agagtttgaa
     5881 acctttaacc gtcagcttaa cgcggttaac cgtcacaccg gcagcaaact ggttaacgcc
     5941 gtgcagcagg atgttcacgc tatccttcag ctgggtgaag cgcagatcga gaaatctgcc
     6001 cgtgcattga ttgatgccgc acgtaacgaa gccgacgaaa aactgtctgc cgagctgtct
     6061 cgtctggaag cgctgcgcgc agtgaacccg aacattcgtg acgacgaact gaccgccatt
     6121 gagagcaacc gtcagcaggt aatggaaagc ctggatcagg caggctggcg tctggatgcc
     6181 ctgcgtttga tcgttgtaac gcatcagtaa cggagccgaa aatggggatg gaaaactaca
     6241 atccaccgca ggaaccctgg ttggttatcc tgtatcagga tgaccacatt atggtggtta
     6301 acaagccgag cggtttgttg tctgtgccgg ggcgtctgga agagcacaaa gacagcgtga
     6361 tgacgcgcat tcagcgtgat tatccgcagg cagaatcggt gcatcgtctg gatatggcta
     6421 ccagcggcgt gattgtagtg gcgctgacca aagccgcgga gcgggagtta aaacgccagt
     6481 tccgcgagcg cgagccgaaa aagcagtatg tagcccgcgt ctgggggcat ccatcccctg
     6541 cggaaggtct ggtggatctg ccgctgattt gcgactggcc aaaccgcccg aaacagaaag
     6601 tctgttacga aacgggtaaa cctgcgcaga cggaatatga agtggtggag tatgcggcgg
     6661 ataacacggc aagagtggtg ttaaaaccga ttaccgggcg ttcgcatcag ctgcgtgtgc
     6721 atatgctggc gctgggtcat ccgattctcg gcgatcgttt ttatgcatca ccagaagcga
     6781 gagcgatggc accgcgtttg ttgctacatg cagagatgtt gacgattacc catccggcgt
     6841 atggcaatag tatgacgttt aaagcgccag cggattttta atcgtcacgc tggcgcagtt
     6901 cggtaggcgg ataaggcgtt cacgccgcat ccggcaattg tgcgccgatt gcctgatgcg
     6961 acgcgggccg cgtcttatca ggcctacagt ttaaaactta tttaaaccct ttctgctgct
     7021 ttatcagctc atatgcctgc tgaatttcct gcgctttctg cttcgccatc tccatcatct
     7081 caggcggcaa acctttcgcc accagcttat cgggatggtg ttcactcatc agcttacggt
     7141 aggcacgttt gatggtggtc gcatcatccg tcggcttcac gcccagcaca ttacaggcat
     7201 cttccagcgt cgggccacgc tgcgcttgct gccagttacc accgccagat tgctgctgat
     7261 aaccgccgcc aaactgtgca ccgccctgca tcatgcgcaa aaactggtca aactgggcgc
     7321 gggagatccc taattcttca gcaatgacat acagcaccgc ccgttcattc gggtgcagtg
     7381 aaccatcagc aaacgccgcc tgaatctgga tctccagaaa catacgaatt aagtcaaaac
     7441 gaccaaagca gacactgcga aactggcgca tcttttcgcg cagcgggtaa ttatctgatt
     7501 ttcccacccg gaacgcgttt tgcgccgcag tacgggaagc gccatgaagg ttcattcggt
     7561 ccatcaactg gctggcgata tgaatatcag cctccgtgac gcgacctttg gatttggtta
     7621 aatgccccat cacttcaaaa gtggtggcaa aaaacagcgc ctgacgctca cgctggttgg
     7681 cgaaccacgc cattttacgg ctacgggctt tatcaaacat atggccaatt aacaggccta
     7741 acactacgcc ccaaaagccg ccgcccatca gtaaggccac ggccacgcca atgatttttc
     7801 cccaatactg catatattcc ccaaatcgac acacggatat cagggctatc tcccacaata
     7861 taaaggtgct tttaccgttt tccggctgcg gtcaattgtg ggacatcgcc tataatttgc
     7921 attatcatac ctgtcattca atgccgtgcc taacaccact gacgctattc ggacaggatt
     7981 aacactagcg tagagatgac gagtacgtta gtctctgacc gtttgtcacg cgcaacgtta
     8041 ccgatgatgg aacaataaaa tcaacgtatg aaaaaacgta tccccactct cctggccacc
     8101 atgattgcca ccgcccttta tagtcaacag ggactggcag ccgacctcgc ctcacagtgc
     8161 atgttgggcg tgccaagcta tgaccgtcct ctggtacagg gcgataccaa tgacttaccc
     8221 gtgactatca atgctgacca cgcgaaaggg gactacccgg atgacgccgt gtttactggc
     8281 agcgtggata tcatgcaggg taacagccgt ctgcaggccg acgaagtgca gctccatcaa
     8341 aaagaggcac caggacaacc ggagccggta cgtaccgttg atgcgctcgg taatgtccat
     8401 tacgacgata accaggtgat cctcaaaggg ccgaaaggct gggcgaatct gaacaccaaa
     8461 gataccaacg tctgggaagg tgattaccag atggtgggtc gccagggtcg cggtaaagcg
     8521 gacctgatga aacaacgtgg cgaaaaccgc tataccattc tggataacgg tagctttacc
     8581 tcctgtctgc cgggttctga cacctggagc gtggtaggta gcgaaattat tcatgaccgc
     8641 gaagaacaag ttgcggagat ctggaacgcc cgctttaagg tgggtccggt accgatcttt
     8701 tatagcccct atttgcagtt gccggtgggt gacaaacgtc gctctggttt cttgatcccg
     8761 aacgccaagt acaccaccac caactacttt gagttctacc tgccatatta ctggaacatc
     8821 gcgccaaata tggatgccac catcacgccg cattatatgc atcgtcgtgg caacatcatg
     8881 tgggagaacg aattccgcta cctctcccag gcgggcgctg gcttgatgga actggactat
     8941 ctgccttcag ataaagtcta tgaagatgaa cacccgaacg atgacagttc acgtcgttgg
     9001 ttgttctact ggcaacactc cggggtcatg gatcaagtgt ggcgtttcaa cgtcgactac
     9061 accaaggtca gcgatcctag ctacttcaat gatttcgata acaagtacgg ttccagtact
     9121 gacggctacg caacgcaaaa attcagcgtt ggctatgcgg tgcaaaactt caatgccacc
     9181 gtttcaacca agcagttcca ggtctttagc gagcagaaca ccagtagcta ctcggcagag
     9241 ccgcagttag acgttaacta ctaccagaat gatgttggtc cgtttgatac gcgtatttac
     9301 ggccaggcag tgcactttgt taacaccaga gacgacatgc ctgaagcaac ccgtgttcac
     9361 ctggaaccga ccatcaattt gccgctctct aataactggg gcagcatcaa taccgaagcg
     9421 aagttgctgg caacccatta tcagcaaacc aatcttgact ggtataactc cagaaacacg
     9481 accaagctgg acgaatccgt taaccgcgtt atgccgcaat tcaaagttga cggcaaaatg
     9541 gtctttgaac gcgatatgga aatgctggct ccgggttata cccaaacgct ggaaccgcgc
     9601 gcgcagtatt tgtacgtgcc gtatcgcgat cagagcgaca tctataacta cgactcgtct
     9661 ctactgcaat ctgactactc tggcctgttc cgggaccgga cttacggcgg tcttgaccgt
     9721 attgcctccg ctaaccaggt gacgaccggt gtcacatctc gcatatatga tgatgctgcc
     9781 gttgaacgtt ttaatatttc cgttggtcaa atctactatt tcacggagtc tcgcactggc
     9841 gatgacaaca taacatggga gaatgacgac aaaacgggct cactggtgtg ggcaggcgat
     9901 acttactggc gtatctccga gcgttgggga ttgcgtggcg ggattcagta cgatacacgt
     9961 ctggataacg tagcgaccag taactccagc attgaatacc gtcgggatga agaccgtctg
    10021 gtacagctga attaccgtta cgccagcccg gaatatattc aggctacgct gcctaagtac
    10081 tattccactg ctgagcaata taagaatggt atttcgcagg taggtgctgt cgccagctgg
    10141 ccaattgccg atcgttggtc cattgttggg gcctactact acgacaccaa tgcgaacaag
    10201 caagccgact ctatgttagg tgtgcaatac agctcctgct gctatgcaat tcgcgtcggt
    10261 tacgagcgga agctgaacgg ttgggataac gataaacaac atgcggtata tgacaacgca
    10321 atcggcttta acatcgaact tcgcggcctg agctccaact acggtctggg tacgcaagag
    10381 atgctgcgtt cgaacattct gccgtatcaa aacactttgt gatttgttga tttaccacgt
    10441 aatccgcagt gcggttaatt gaaatggaaa aagtatgaag aactggaaaa cgctgcttct
    10501 cggtatcgcc atgatcgcga ataccagttt cgctgccccc caggtagtcg ataaagtcgc
    10561 agccgtcgtc aataacggcg tcgtgctgga aagcgacgtt gatggattaa tgcagtcggt
    10621 aaaactgaac gctgcgcagg caaggcagca gcttcctgat gacgcgacgc tgcgccacca
    10681 aatcatggaa cgtttgatca tggatcaaat catcctgcag atggggcaga aaatgggagt
    10741 gaaaatctcc gatgagcagc tggatcaggc gattgctaac atcgcgaaac agaacaacat
    10801 gacgctggat cagatgcgca gccgtctggc ttacgatggt ctgaactaca acacctatcg
    10861 taaccagatc cgcaaagaga tgattatctc tgaagtgcgt aacaacgagg tgcgtcgtcg
    10921 catcaccatc ctgccgcagg aagtcgaatc cctggcgcag caggtgggta accaaaacga
    10981 cgccagcact gagctgaacc tgagccacat cctgatcccg ctgccggaaa acccgacttc
    11041 tgatcaggtg aacgaagcgg aaagccaggc gcgcgccatt gtcgatcagg cgcgtaacgg
    11101 cgctgatttc ggtaagctgg cgattgctca ttctgccgac cagcaggcgc tgaacggcgg
    11161 ccagatgggc tggggccgta ttcaggagtt gccgggtatc ttcgcccagg cattaagcac
    11221 cgcgaagaaa ggcgacattg ttggcccgat tcgttccggc gttggcttcc atattctgaa
    11281 agttaacgac ctgcgcggcg aaagcaaaaa tatctcggtg accgaagttc atgctcgcca
    11341 tattctgctg aaaccgtcgc cgatcatgac tgacgaacag gcccgtgtga aactggaaca
    11401 gattgctgcc gatatcaaga gtggtaaaac gacttttgct gccgcagcga aagagttctc
    11461 tcaggatcca ggctctgcta accagggcgg tgatctcggc tgggctacac cagatatttt
    11521 cgatccggcc ttccgtgacg ccctgacccg cctgaacaaa ggtcaaatga gtgcaccggt
    11581 tcactcttca ttcggctggc atttaatcga actgctggat acccgtaatg tcgataaaac
    11641 cgacgctgcg cagaaggatc gtgcataccg catgctgatg aaccgtaagt tctcggaaga
    11701 agcagcaagc tggatgcagg aacaacgcgc cagcgcctac gttaaaatcc tgagcaacta
    11761 atggttaaaa cccaacgtgt tgtgatcact cccggcgagc ccgccgggat tggcccggac
    11821 ttagttgtcc agcttgcaca gcgtgagtgg ccggtcgaac tggttgtttg tgccgatgcc
    11881 actctcctta ccgaccgggc agcgatgctc ggtttgccgc tcaccctccg cccttattcc
    11941 cccaactccc ctgcacaacc gcaaactgcg ggcacattaa cgctacttcc tgtcgcgcta
    12001 cgcgaatctg tcactgcggg gcagttagcg attgaaaatg ggcattacgt ggtggagacg
    12061 ctggcgcgag cgtgtgatgg ctgtctgaac ggtgaatttg ctgcgctgat cacaggcccc
    12121 gtgcataaag gcgtcattaa cgacgcaggt attccgttta ctggtcatac cgagtttttc
    12181 gaagagcgct cgcaggcgaa aaaagtggtg atgatgctgg cgaccgaaga acttcgcgtg
    12241 gcgctggcaa cgacgcattt accgctgcgc gatatcgcag atgctatcac ccctgcactt
    12301 ttgcacgaag tgattgctat tttgcatcac gatttgcgta caaaatttgg tattgccgaa
    12361 ccgcgcattc tggtctgcgg gctgaatccg cacgcgggcg aaggcggtca tatgggtacg
    12421 gaagagatag acaccattat tccggtgctc aacgagctgc gggcgcaggg gatgaaactc
    12481 aacgggccgc tgcctgccga taccctgttt cagccgaaat atctcgataa cgccgacgcc
    12541 gtgctggcga tgtaccacga tcagggtctt cccgtgctaa aataccaggg cttcgggcgc
    12601 ggtgtgaaca ttacgctggg cctgcccttt attcgcacat cagtggacca cggcaccgcg
    12661 cttgaactgg cgggacgtgg cgaagccgat gtcggcagtt ttattacggc gcttaatctc
    12721 gccatcaaaa tgattgttaa cacccaatga ataatcgagt ccaccagggc cacttagccc
    12781 gtaaacgctt cgggcaaaac tttctcaacg atcagttcgt gatcgacagc attgtttctg
    12841 ccattaaccc gcaaaagggc caggcaatgg tcgaaatcgg ccccggtctg gcggcattga
    12901 ccgagccggt cggcgaacgt ctggaccagc tgacggtcat cgaacttgac cgcgatctgg
    12961 cggcacgtct gcaaacgcat ccattcttag gcccgaaact gacgatttat cagcaggatg
    13021 cgatgacctt taactttggc gaactggccg agaaaatggg tcagccgctg cgtgttttcg
    13081 gcaacctgcc ttataacatc tccacgccgt tgatgttcca tctgtttagc tatactgatg
    13141 ccattgccga catgcacttt atgttgcaaa aagaggtggt gaatcgtctg gttgcaggac
    13201 cgaacagcaa agcgtatggt cgattaagcg tcatggcgca atactattgc aacgtgatcc
    13261 cagtgctgga agtaccgccg tcagccttta caccaccacc caaagtggat tccgccgtcg
    13321 tgcgcctggt tcctcatgca acgatgcctc acccggttaa agatgttcgc gtgttgagcc
    13381 gcatcaccac tgaagccttt aaccagcgtc gtaaaaccat tcgtaacagc ctcggcaacc
    13441 tgtttagcgt cgaggtgtta acgggaatgg ggatcgaccc ggcgatgcga gcggaaaata
    13501 tctctgtcgc gcaatattgc cagatggcga actatctggc ggagaacgcg cctttgcagg
    13561 agagttaacg atgatcaatt cgccccgagt gtgtattcag gttcaaagcg tctacattga
    13621 ggcacaatct tcacctgata atgaacgtta cgtttttgct tataccgtaa ccatacgcaa
    13681 tctggggcga gcgccagtgc agttgttggg gcgttactgg ctgatcacca atggcaatgg
    13741 ccgtgaaacc gaagtccagg gcgaaggagt ggttggcgtc cagccactta tcgcgcctgg
    13801 cgaagagtac cagtacacca gcggtgcaat cattgaaacc ccgctgggca ccatgcaggg
    13861 tcactacgaa atgatcgatg aaaatggcgt ccctttcagc atcgacattc ccgtattccg
    13921 actcgccgtt cccacactca ttcattaaaa gaatatggcg acatacctta ttggcgacgt
    13981 tcatggttgt tacgatgaac tgatcgcatt gctgcataaa gtagaattta cccctgggaa
    14041 agataccctc tggctgacgg gcgatctggt cgcgcgcggg ccgggttcgc tggatgttct
    14101 gcgctatgtg aaatccttag gcgacagcgt acgtctggtg ctgggcaatc acgatctgca
    14161 tctgctggcg gtatttgccg ggatcagccg caataaaccg aaagatcgcc tgacaccgct
    14221 gctggaagcg ccggatgccg acgagctgct taactggctg cgtcgccagc ctttgctgca
    14281 aatcgacgaa gagaaaaagt tggtgatggc ccacgcaggg atcacgccgc agtgggatct
    14341 acagaccgcc aaagagtgcg cgcgcgatgt agaagcggtg ctatcgagtg actcctatcc
    14401 cttctttctc gacgctatgt acggcgatat gccaaataac tggtcaccgg aattgcgggg
    14461 gctgggaaga ctgcgtttta tcaccaacgc ttttacccgt atgcgttttt gcttcccgaa
    14521 cggtcaactg gatatgtaca gcaaagaatc gccggaagag gcccctgccc cactgaaacc
    14581 gtggtttgcg attcctggcc ctgtcgctga agaatacagc atcgcctttg gtcactgggc
    14641 atcgctggag ggcaaaggta cgccggaagg tatatacgcg ctggataccg gctgctgctg
    14701 gggtggtaca ttaacctgcc tgcgctggga agataaacag tattttgtcc agccgtcgaa
    14761 ccggcataag gatttgggcg aagcggcggc gtcttaaaca cagcctgata taggaaggcc
    14821 ggataagacg cgaccggcgt cgcatccggc actagccgta aattctatac aaaattaccg
    14881 ccgctccaga atctcaaagc aatagctgtg agagttctgc gcatcagcat cgtggaattc
    14941 gctgaatacc gattcccagt catccggctc gtaatccggg aaatgggtgt cgccttccac
    15001 ttctgcgtcg atatgcgtca gatacagttt ctgcgctttt ggcaggaact gctcataaac
    15061 gcgaccgccg ccaatcacca tgatttctgg tacgtcacca cacgccgcga tggcttcatc
    15121 caccgacttc acccacgtta cgcgatcgtc cgtacccggt tgactgctga ggataatatt
    15181 tttgcgtcct ggcaacggac gaccgattga ttcccaagta tggcggccca taatcacggg
    15241 tttatttaag gtgttgcgtt taaaccaggc gagatcggca ggcaggttcc acggcatggc
    15301 gttttccatg ccgataacgc gatctaccgc taacgccgca atcagactga tcattgagat
    15361 ttcccgataa aaaaaattgt cgccactata cgtaaagcgt aaaccgtcgt cgactggtgc
    15421 gaggatgatg ttgaggaaaa ttttatattc tgctggcgag tccacgctct ctccctggac
    15481 tcgccgcatt acaatgaaac aaaaacaaac agttagctgt aaagtgtgat ttacgtcact
    15541 ctttattagg atgagggttt cgtttccggt tcatccgcca tgttgccggt atgtttacct
    15601 tcttcggttc cctgccagcc atgtcgttga attaatgaca gatgttcgcg gtcctcggta
    15661 atgatctcac ttaacatcgc gctggtgcgt ttatagaccg ccgcgcgggc tttggtgtcg
    15721 ttctcaacca ttgccatctc ttccaccatc tgaatattaa agcggcggaa cacatcggca
    15781 cgttctcgcg cttcatacgg ccccagacct aaactttcca gtgccagacg cccggttttc
    15841 agcgcacctt cgaaggtttc acgctccggc ttttcaacgc ctgcctgacg caaacgaatg
    15901 tagtggtcga catcgcgggc gcgggcaata atctgcaaat gcgggaagtg ttctttcacc
    15961 atctctgtca gttgcaggtt ggtttgcgga tcgtcgatgg cgttaatcag cacttccgct
    16021 ttcgccgctc ccgcagattc cagtaaatcc atccgcgtgg catcgccata aaacactttc
    16081 ataccaaatt tacgcaaggt ttcgatatgg tccggatcgt gatcgagcac caccattttc
    16141 accccgctgg agagcagtaa acgtccggtg atctgcccaa aacgaccgaa tccggcaatg
    16201 attacccgcg gctgttcttc gtcgatctca tcagcttcac gcgcctcttc agtagaagat
    16261 tgctcaaggc gattgaggat caccagcaga atcggcgttg ccgccatcga cagcgccacc
    16321 gccagggtca gcgatttcgc ccactccggt tccagcacat tcgccatctg cgccgtgcca
    16381 aataccacaa aggcaaactc actgccctgc cctaacaaca ccgcaaacca acgacgctgt
    16441 ttatttggca cttgcaacgg tcgggcaatc agccacagca tggcgatttt gatgatgagg
    16501 aaaccgagca gcaaaatgac aatgcgcaat gggttttcaa gcagcgtgcc aaagtctatc
    16561 gacatgccaa caccgatgaa aaacagcccc aacagcaaac ctttaaatgg ttcgatatcg
    16621 ctctccagcg catgacggta ttccgagctt gccagcagta cgcccgccag aaacgcgccc
    16681 atcgccatcg acaagccgac ctcttccagc agcaaaccaa agccaaacac gaggaataac
    16741 gccacggcac taaacacttc ccgcaagcca gagcgggcta caaaacgcag cgccggacgc
    16801 gtgacatagc gccccagcaa taccaccagc accagcgcac ccgccacttt taacgccgag
    16861 agagcaaatg cgcccatcgt cgtcgaggca ctgctcgttg ccagtagcgg aatcatcgcc
    16921 accagcggga tcgccgcgat atcctggaac agcagcaccg caaaggcact gcgacccatt
    16981 tgcgtcacca tcagattgcg ttcattcatc gcctgcatgg caatcgccgt agaggagagc
    17041 gccagcgtca tgccgatcaa ttccgcgacc tgccagtgca gcccaagtaa catgcagaac
    17101 agccccagca ggccgccgca aatcaccatc tgcaatgcgc caccgccgaa cactgccgca
    17161 cgcagcttcc acagcctttg tggatcgagt tcgaggccga taataaacag catcagcacc
    17221 accccaatct cggcaaagtg cagaatagat tcggcatcgg tcaccagtcg cagcccccac
    17281 gggccaataa tgcagccggc gatcaggtag ccaagtaccg atcccagacc aagacgtacc
    17341 gcaatgggta caatcagcgc tgccgaaccg agataaatca gcgcctgaat cagcgtatgg
    17401 ctatccatga tgggcctcct gccattccag cagacgttgc ttatagtgac gcgcctgccc
    17461 ttcgagggtt tcgtcgtcac aaataaaggt gcagtgcatg gcaaacggtg gcagccagtt
    17521 cagcccgcag tagattgccg tcgcctgtag cggctgcgac agcacatcaa agcccggatg
    17581 cgcaccaatt tcaaaatggc tttccccgcc gccggtcgtc accgcccaca gcaaatattt
    17641 gccatgcagc gccttgccgc cgtgaccgta agcccagccg tgcgacaaaa ctttatcgat
    17701 ccaaagttta agaagcggag gaatgctgta ccactgcatc ggatgctgcc agacgatcag
    17761 atcggcgcga gacagcgcct cctgctcggc ggcaatatcg atattgaagt caggatagag
    17821 ttgataaaga gagcgaattt cgacgccttc cagcgtcctt gcctgttcaa gcatccgttt
    17881 attcgcatgg gaatgatgcg gatacggatg cgcataaatt ataagaatca taggtagccc
    17941 gcgtgattac gcctttgttt tcccacaaag tgtagtcata tattcacgcg gcttatagtc
    18001 aataatattg attcagaaaa gagagaaatc ttaacaattt accccattct ctgcccgcgg
    18061 atcgtcatat tgctggtttg cgccagtgac atccctcgag tctccggggc aaacgctacg
    18121 gaaatcaaca agccaaacag cgagataccc gcctccatta gcatcgtgtt actgataccg
    18181 taattattga taaagatcgg cagtgcccag gtcgaaacaa tggtgccaat acggcttaag
    18241 gacataatca cgcccacggc agaggcgcgg atatctgtcg ggaagagttc attaggatag
    18301 agccactgca aattacccgg cccgccagag aaaaaggcat acaccgcaaa ggccatcact
    18361 accagccaga tccccatatc cgggatcagc cccaaaaccg ccagcgccag cgtcatcatg
    18421 gcaaagctgc caatcaacaa tggacgccgt ccggcagtgt ttaaccacag catcggcgga
    18481 atacagccga gcataaagaa caggctaatc accacgttcc ccagcgccgc gtttttgcca
    18541 acacccaatc ccaacaaacc aacgatttgc gggccaaagg tgtaaatggc gaacattggg
    18601 atcacctggc aggtccagat ggcggcaaca aacagaacaa aaggaaaatg gcggcgatta
    18661 aacagatcgc gaaaacgggt ttgctacggc tgctcttcat cgaaagccac cggttcgcca
    18721 aacagtttta tcatcatctc ttcgcactct tttactcgcc ctttgcgtaa taaccagcgg
    18781 ggagattcag gcagttcgaa tcgaccaatc aaaatcaaca aacaggggat cgccgcgcta
    18841 cccagcatcc agcgccagcc gccttccaca tcataaagcc agtagccgac cagatcggcg
    18901 caggtcgcgc cgacatacca catggcggcg ataaagctga tggaaaatgc ccgctgacgg
    18961 gtactggaga actcggtgat cattgaggtg gcgatgggat aatctgcacc gatgacaatg
    19021 ccgataagta cccgcatcac caacagttcg acgggggatg aaacaaacat cgtcgccacc
    19081 gatatcacgc cgatggcgat gatatcaatg aggaacattt tgcgccgtcc tactttatcg
    19141 gaaatatagc caaacagcga tgtgccaacg aacagcccgg cgagcgttcc cgcgcccaac
    19201 aagccaatcc agtcagcgtc cagttttagc gccggagtca gttgctccag cgccacgcca
    19261 atcattacca gtacataacc atccagaaac ggaccaccgc ttccccacag caaaatgcgg
    19321 cggtgaattg aggagaattt gagatcgtca aagtttctgg acggttgcat gggttattcc
    19381 tgtttttttg caatgagagt cagtacggcg ttacgtatca aaccaacatc agccgtaacg
    19441 gaactccaca ccaaaggtgc cgcgcgggta ttcccactgt tccagcgccg accccagccc
    19501 cagaatgcga caggtgccgc actccagaca tccggcgtaa tcgaagcgca cactgccgtc
    19561 atcctgcttc ttgtacagac ctgcggggca cgctttcacc agcagctcca gggcctgttt
    19621 atcggcatcg gccttcacaa caatgtgcgg atgctcttca tcgacattga atttattgac
    19681 gcccagtttg acgtccacat tgacgggaga agtcataaaa cggtcactcc tttcatgcca
    19741 tccttgatca gattgatgaa gcccactttc ttgccgtggc ggaggatttt cttgcgcatc
    19801 agttccggcg cactgccgtc aatggtgaac aggtcacgcg ccacgcccac cgccagttcc
    19861 gggtagccgc taaacatgcg tgggttatca aggaaggccg gtagtttctg gtacatacgc
    19921 atatcgcgca gcgggccact ctcaagatgc tgacgatatt ccgccagttt ttgcttactg
    19981 aaatcgtcgc ttttcatcgc tgaaagcacg gtttttgctg cggcttcccc ggcggcaatc
    20041 gccagatcca taccgcgaat ggtaaaaccg aggttcatac acattccggc ggcatcaccg
    20101 gcaatcaata cgccgtcacc aaccaactcc ggcagcatgt tgatgcctgc ttccggcact
    20161 acgtgagcgg aatattccac cagcttgccg cccgcgatca gcggtgccac tgccggatgc
    20221 tgtttgaaat cttccagcat ttgcggcacc gatttttttg cgtcatgcag atgatgcaga
    20281 ccacaaacca gccccagcga cagggtattt tcattggtat aaaggaagcc gccgcccatc
    20341 aggccatcgg tgggtgatcc cgcaaacaga caagccgcgc cctgattacc ctgcaactga
    20401 aaacggtctt cgattaccga cttcggtaac tcgatcagtt ccttcacgcc aaccgccaca
    20461 tccgtcggtt taacgcgttt tgccatcccc agcttttcgg caaggatgga gttcactcca
    20521 tcagcaagga tcaccgtttt cgcttcaatc acatcgccat cggcttctac accgacgact
    20581 ttgccatcgc gctgtacgag gttatcgacg cggatcccgg taattaactg cgcgcccgct
    20641 tcttcggcct gctccatcag ccaggcatca aatttactgc gcaaaacgga gtaagaacgc
    20701 tgggatggcg aggtttcgtc accgttgcag tagttcatag tcatcgctga cttttccgtc
    20761 ataaacgcga gtttttcatg ggtgatcagg cgttctaccg gggcggattc ggcgaaacca
    20821 ggaataatgt gttccaggct gtgggcatag agacgcccgc cggtgacgtt cttggcacct
    20881 gcggaattgc cacgctcgat aactaacact tgcgcccctt cgcgggcgag caccagtgcg
    20941 gcaaccgaac cggcaagccc agcaccgacg atgatggcgt caaagatatc ttcggacata
    21001 agagctccct gtcagcggca gggccaaaat gcagccctgc cagagtggat caacgcgcta
    21061 aagctgcggt cagcgccgga aggatcttca cggcgtcacc aacaatgccg taatccgcgt
    21121 actggaagat cggcgcattt ttatctttat tgatggcgaa aatggtttgc gatgcattag
    21181 caccaaccat gtgctggatc tgcccggaga tccccaccgc caggtacagt tcaggtttca
    21241 gcatcaggtt ggagataccg acatagcgtt cgtgctccat ccatttttcg ttttccgcca
    21301 ccggacgaga acaggccaac tccgcaccta tcgccttgca aagctgttct gccagcgcaa
    21361 tgttctcttt gctgccaata ccgcgaccga cgctgaccac cagacgggct ttgtcgagat
    21421 cgacgctgtt gctctggcgc gcctgggttg ccgtgcgggt aatcgccaca gccggagcct
    21481 gccactccac ggtgtgcgtt tcgccagtgc gtgacgcgtc tggctgagcc gcatcgaacg
    21541 tgccgctgct gatggtcagt accgcatacg gcgtggcaat gcgttcttcg ccaatcgcca
    21601 gaccaccgta aaccatatgt ttcactgttg ctttaccgtc ctgtacgctg acggtgctgg
    21661 catcgttaga caccgccgct ttaaggcgat aacccagttt tgccgccagt aatttgccgc
    21721 gacgggtgtt tggcagcagc accaggccgt ctgcgccgtg ctggcgaata gtgtcagcca
    21781 tgacaccggc gtaatcttcg atcatccgat cgtccggttt gccgtttaat ttccagacat
    21841 gattagcgcc gagctggatt gcctgtgcgc cgtcggcatc attgaggaca aaggtgttga
    21901 tttgattagc taaagcctgc gcaccgttca tcagttccgg cagacgagaa ggggtatcgc
    21961 tgaataccca gacttgagaa aacgtgttca tagcatcccc tgtaattaaa tgactttgcg
    22021 aagattttca gcaaatgcgg cgatctgttc ttcgccgtcg ccttcaatca cgatgcgctg
    22081 acgttcgcgc tgtttcggcg cggcaacctg ttgttctgac caggctgcct ctgcgttaaa
    22141 accaatatcc gccgccgacc atacctggac gggctttttc gccgcgccga gaatggcttt
    22201 catcgaagga atttgtgggg aattgatatc agtggaaaca gcaacaaccg caggcagcgg
    22261 aatgcttaag gtttcggttt catcttccag ttcgcgctca acggtgaggg tatctgccgt
    22321 cagggagata attttgctga cgccgttaac tgccggaata ttgaggattt cgcccaccag
    22381 cagaccaacc tgctgggcat aaaggtcgga agaaccatcg ccacagagga tcaaatcaaa
    22441 gcctgctttc tgggcggctg cagccagtgc gctcgccgtt tgttgcggca gtgcctgctc
    22501 gaactggtca tcaatcacca caatcagttc atccgggccg cgcgatagta catctttacg
    22561 ccctttggcg ttggtcaggg ctttaccgcc cacacttaag gccgtcacct gcgcctctgc
    22621 tgcctgttgc tttagctggc aagccgcttc aatagcgttc agatcgtatt ggcttatttt
    22681 ggcatcggct ttgctgaagt ctaatgaacc atcagcatta ttgaccgcaa tatcctgttc
    22741 atcaggcacg cacttatagc aagtaataat cttcattgca tctccagaaa tcatgaagga
    22801 aacgtcgttc atatcggtgg tgttaattac agatatcacg ccctttaatt tggaaataaa
    22861 actttaatca ccaatattga aaatgtcaca cgcagataaa tccaactttc aatattgtta
    22921 agttcctcac caatattgaa aacatggcgt agaaaaaaga aattttcaat attgctttat
    22981 ggatcaccaa tattgaaagt cagatgctat ttttttactc acaacacaac ataacaaact
    23041 gattaataat caaaattaac gagaaaagcg ccctctattc acccgaaaaa aaatgtgata
    23101 acaatcacag aatgcagctt attgaatacc cattatgagt tagccattaa cgcgtccacg
    23161 aggttaataa taattatatc aaatgttaac aaaaataaaa caaacgggaa cgcaaaataa
    23221 atattcgttt tcacagtgga attaactcat gaagaatgaa aagagaaaaa cgggaataga
    23281 accgaaggtt ttctttccgc cgttaataat cgtcggcata ctttgttggc ttacagtcag
    23341 agatctggat gcagcgaatg tcgttattaa tgctgtattc agttacgtca ccaatgtatg
    23401 gggatgggca tttgaatggt atatggtggt gatgcttttc ggttggttct ggctggtgtt
    23461 tggcccgtat gccaaaaagc gtttaggtaa cgaaccacct gaatttagta ccgccagttg
    23521 gatctttatg atgttcgcct cctgtacgtc tgctgccgta ctgttctggg gatcgattga
    23581 gatctactac tacatctcca ccccgccgtt tggcttagaa ccgaactcga caggggcgaa
    23641 agagttgggg ctggcttaca gcttgttcca ctggggacct ctgccgtggg ccacttacag
    23701 cttcctttca gtcgccttcg cttacttctt ctttgtccgc aaaatggaag tgattcgccc
    23761 cagctcgaca ctggtgccgc tggtaggtga aaaacacgcc aaagggttgt tcggcactat
    23821 cgtcgacaac ttctatctcg tcgccttgat cttcgcgatg ggtaccagtc tgggccttgc
    23881 cacgccgctg gtgaccgagt gtatgcaatg gttgtttggc attccgcata ccctgcaact
    23941 ggacgctatc atcattacct gctggattat cctcaacgcc atttgcgtcg cttgcggtct
    24001 gcaaaaaggg gtacgtatcg ccagtgacgt gcgtagttac ctgagcttcc tgatgctggg
    24061 ttgggtgttc attgtcagcg gtgccagctt catcatgaac tacttcaccg attcggtggg
    24121 gatgttgctg atgtatctgc cgcgcatgtt gttctatacc gatcccatcg ctaaaggcgg
    24181 cttcccgcag ggctggaccg tgttctactg ggcatggtgg gtgatttatg ctatccagat
    24241 gagtatcttc ctcgcccgca tctcccgtgg tcgtactgtg cgtgaactgt gcttcggcat
    24301 ggtgctgggg ctgacagcgt caacctggat cctgtggact gtactcggta gtaacactct
    24361 gctgttgata gataaaaaca tcatcaacat tccaaatctg atcgaacagt acggtgtggc
    24421 gcgcgccatc attgaaacct gggccgctct gccactcagc accgccacca tgtggggctt
    24481 cttcatcctc tgctttattg ccaccgttac gctggttaac gcctgctctt ataccctggc
    24541 gatgtccact tgccgcgaag tacgcgatgg tgaagaacca cctctgctgg tgcgtatcgg
    24601 ttggtcaatt ctggttggca ttatcggtat tgttctgctg gcgctcggcg gcctgaaacc
    24661 gattcaaacc gccattatcg ccggaggatg cccgctgttc ttcgtcaaca ttatggtgac
    24721 gctctccttt attaaagacg cgaaacagaa ctggaaagat taattaaccc ccaaaatatc
    24781 aagaggttga aagatggatt ttaatttaaa tgatgagcag gaactgtttg tcgccggtat
    24841 ccgcgaactg atggccagcg aaaactggga ggcctatttt gccgagtgcg accgtgacag
    24901 cgtctacccg gaacgttttg tcaaagcact ggcggatatg ggtatcgaca gtctgctgat
    24961 ccctgaagag cacggtggtc tggacgcggg gtttgttact ctcgccgccg tgtggatgga
    25021 gctgggacgt ctgggggcac caacctatgt gctgtaccag ttgccgggcg ggttcaacac
    25081 cttcctgcgc gaaggcacac aagagcagat cgacaaaatt atggctttcc gcggcaccgg
    25141 taagcagatg tggaactcag cgattaccga accgggcgcg ggctccgacg tgggtagcct
    25201 gaaaacgact tatacccgta gaaatggtaa gatttatctt aatggtagta agtgttttat
    25261 taccagcagc gcctacaccc cgtacatcgt ggtgatggcg cgcgacgggg cttctccgga
    25321 caaacctgtc tacaccgaat ggtttgttga tatgagcaaa ccgggcatca aagtgaccaa
    25381 acttgaaaag ctcggtctgc gtatggatag ctgctgtgaa atcacctttg acgacgtgga
    25441 actggacgag aaagacatgt tcggtcggga aggtaacggc tttaaccgcg tcaaagaaga
    25501 gttcgaccat gaacgtttcc tggtagccct caccaactac ggtacggcga tgtgcgcttt
    25561 tgaagatgcg gcgcgctacg ccaatcagcg cgtgcagttt ggcgaggcta ttggtcgttt
    25621 ccagttgatt caggaaaaat tcgcccacat ggcgatcaaa ttaaactcca tgaaaaacat
    25681 gctgtatgaa gcagcgtgga aagcagacaa cggcaccatc acctctggcg atgcagcgat
    25741 gtgcaaatac ttctgcgcca atgcggcatt tgaagttgtg gatagcgcaa tgcaggtgct
    25801 gggcggtgtc gggattgcgg gcaaccaccg catcagccgc ttctggcgtg atctgcgtgt
    25861 agaccgcgtc tccgggggat ctgacgaaat gcagatcctg acgctgggtc gcgcggtgct
    25921 gaagcaatac cgctaagtcc gtgcgctgcc tgatgcgacg ctaatgcgtc ttatcaggcc
    25981 tacgggagca cgaatgtagg ccggataaag cgtttacgcc gcatccggca gtcatgcgct
    26041 acataaattt ttcaggagct aataatggat catctaccca tgccgaaatt cgggccgttg
    26101 gccggattgc gcgttgtctt ctccggtatc gaaatcgccg ggccgttcgc cgggcaaatg
    26161 ttcgcagaat ggggtgcgga agttatctgg atcgagaacg tcgcctaggc cgacaccatt
    26221 cgcgttcaac cgaactaccc gcagctctcc cgccgcaatt tgcacgcgct gtcgttaaat
    26281 attttcaaag atgaaggccg cgaagcgttt ctgaaattaa tggaaaccac cgatatcttc
    26341 atcgaagcca gcaaaggtcc ggcctttgcc cgtcgtggca ttaccgatga agtactgtgg
    26401 cagcacaacc cgaaactggt tatcgctcac ctgtccggtt ttggtcagta cggcaccgag
    26461 gagtacacca atcttccggc ctataacacc atcgcccagg ccttcagtgg ttacctgatt
    26521 cagaacggtg atgttgacca gccaatgcct gccttcccgt ataccgccga ttacttttct
    26581 ggcctgaccg ccaccacggc ggcgctggca gcactgcata aagtgcgtga aaccggtaaa
    26641 ggcgaaagta tcgacatcgc catgtatgaa gtgatgctgc gtatgggcca gtacttcatg
    26701 atggattact tcaacggcgg cgaaatgtgc ccgcgcatga gcaaaggtaa agatccctac
    26761 tacgccggtt gcggtctgta taaatgcgcc gacggctaca tcgtgatgga actggtgggc
    26821 attacccaaa ttgaagagtg ctttaaagat attggcctcg cacatctact cggtacgccg
    26881 gaaatcccgg aaggcactca gcttatccac cgtatcgaat gcccttacgg cccactggta
    26941 gaagaaaaac tcgatgcctg gctggcggca cataccatcg cagaagttaa agaacgcttt
    27001 gccgaactga atatcgcctg tgccaaagta ctgaccgtac cggaactgga aagcaatcca
    27061 cagtatgttg cccgtgaatc aatcactcag tggcaaacga tggatggtcg cacctgcaaa
    27121 gggccgaaca tcatgccgaa attcaaaaat aaccccggac aaatctggcg cggaatgccc
    27181 tcacatggca tggacacggc tgccattttg aaaaatatcg gctacagcga aaacgacatt
    27241 caggagttgg tcagcaaagg tctggccaaa gttgaggact aaacgcgtgg cgtcagtcgg
    27301 cttgcctgac tagcgcccgc ccaataacaa acgaacaaaa atggatagag gtgcaatgga
    27361 tatcattggc ggacaacatc tacgtcaaat gtgggacgat ctggcggacg tttacggtca
    27421 taaaacggcg ctgatttgtg aatccagcgg cggagtcgtt aaccggtata gttatcttga
    27481 gttaaatcag gagattaacc gcacgacaaa cctgttttat acgctgggga ttcgcaaagg
    27541 cgacaaggtt gctctacatc tcgacaactg cccggaattt atcttttgct ggttcgggct
    27601 ggcaaaaatt ggcgcgatta tggtgccgat taacgcccgc ctgttgcgcg aggaaagcgc
    27661 gtggatcctg caaaatagcc aggcgtgcct gctggtgacc agtgcgcaat tctatcctat
    27721 gtatcaacag attcagcagg aagatgccac tcaattgcgg cacatttgcc tgacagatgt
    27781 ggcacttccc gctgatgatg gcgtgagttc gtttactcaa ctgaaaaatc aacaacctgc
    27841 caccttgtgc tatgcaccgc cgctatcgac tgacgatacg gcggaaattc tcttcacctc
    27901 cggcaccacc tcccgaccga aaggtgtggt gattacccat tacaacctgc gcttcgctgg
    27961 atattactcc gcctggcagt gtgcactgcg tgacgatgac gtctacctga cggtaatgcc
    28021 tgcgtttcat atcgattgcc agtgtactgc ggcgatggcg gcgttttctg ccggggccac
    28081 ctttgtgctg gtcgagaaat acagcgcccg cgccttctgg ggacaggtac agaagtaccg
    28141 cgccaccgtt accgaatgta ttccgatgat gatccgtacc ttgatggcgc agccgccttc
    28201 agcgaacgat cggcaacacc gcctgcggga agtgatgttt tatctcaact tgtcggagca
    28261 ggaaaaagat gcgttttgtg aacgcttcgg cgttcgcttg ctgacgtctt atgggatgac
    28321 ggaaaccatt gtgggcatta tcggcgatcg ccctggcgat aaacgacgct ggccgtcgat
    28381 tggtcgggcg gggttttgct acgaagcgga gatccgcgac gatcacaatc gcccgctccc
    28441 ggcaggtgag atcggtgaaa tctgtattaa aggcgtacca gggaaaacca tcttcaaaga
    28501 gtattttctc aacccgaaag ccactgcgaa agtgctggaa gccgacggct ggctacatac
    28561 cggcgatacc ggatactgcg acgaagaggg ctttttttat ttcgtcgatc gccgctgcaa
    28621 tatgatcaaa cgcggtggcg agaatgtctc ctgcgtggag ctggaaaata tcatcgccac
    28681 ccatccaaaa attcaggata tcgtggttgt gggtattaaa gattcgattc gcgatgaagc
    28741 catcaaagca tttgtggtgc tgaatgaagg tgaaacattg agcgaagagg aatttttccg
    28801 cttctgcgaa caaaatatgg cgaaatttaa agtgccctct tatctggaga tcagaaaaga
    28861 tctgccacgt aattgctcgg ggaaaataat tagaaagaat ctgaaataaa tgaaacagca
    28921 gggaactacc ctgccaacta ataatcacgc tataaaacaa tatgcatttt ttgcagggat
    28981 gttgtcgtcc ctgaaaaagc aaaaatggag aaaaggaatg agtgaatcat tacatctgac
    29041 ccgcaatgga tcaattctgg aaattaccct tgatcgtcca aaagcgaatg ctattgatgc
    29101 aaaaaccagc tttgaaatgg gcgaagtatt tctaaatttc cgtgacgatc cgcaattacg
    29161 tgtcgccatt attaccggtg ccggagagaa gttcttttcc gcgggctggg atttaaaagc
    29221 ggcagcagaa ggcgaagcac cggatgctga ctttggcccg ggtggatttg cgggattaac
    29281 cgaaattttc aatctcgaca aaccggttat cgcagctgtg aacggctatg cctttggcgg
    29341 cggtttcgaa ctggcgctgg cggcagattt tattgtttgt gccgataacg ccagcttcgc
    29401 cctgccggaa gccaagctgg gcatcgttcc tgacagcggc ggcgtgctgc gtctaccgaa
    29461 gatcctgccg cctgccatcg tcaatgaaat ggtgatgacc ggcagacgaa tgggcgcaga
    29521 agaggcgctg cgttggggga tagtcaaccg cgtggttagc caggcggaac tgatggataa
    29581 cgcccgcgaa ctggcgcagc agctggttaa cagcgccccg ctggcgattg cggcgctgaa
    29641 agagatcttc cgcaccacca gcgaaatgcc ggtagaagaa gcgtatcgct atattcgcag
    29701 cggcgtgttg aaacactatc cgtcggttct gcattcggag gatgccattg aagggccgct
    29761 ggcgtttgcc gagaagcgcg atccggtgtg gaaaggacgt taacaaccgt gagctattac
    29821 gcctttgagg ggttaattcc ggtggttcac ccgacggcgt ttgtccatcc cagtgccgtc
    29881 ttgattggcg atgtgattgt gggagccggt gtctacatcg gcccactcgc ctcactgcgt
    29941 ggtgactacg ggcggttgat cgtgcaagcg ggagccaata ttcaggatgg ctgcattatg
    30001 catggctact gcgacactga caccatcgtt ggggaaaacg gccatatcgg gcacggagcg
    30061 atcctgcatg gttgtgtgat tggtcgcgat gcattggtcg ggatgaacag cgtgattatg
    30121 gatggcgcgg tcattggcga agagagcatt gttgccgcca tgagctttgt caaagcaggc
    30181 tttcacggcg agaaacgcca gttgttgatg ggtacgccag cccgcgctgt acgcagtgtt
    30241 agtgacgacg agttacactg gaaacggttg aataccaaag agtatcagga tcttgtcggg
    30301 cgctgtcatg catcgttaca tgaaacacag ccactgaggc aaatggagga aaatcgcccc
    30361 cgtttgcagg ggacgacgga tgtgacgcct aaacggtgat cttgccggat gcggcgtaaa
    30421 cgccttatcc aactctgctg gagagcaacc gtaggccgga taagatgcgc cagcatcgca
    30481 tccggcgata gctgtcggat gcggcataaa cgccttatcc aacccggctg gagagcaacc
    30541 gtaggctgga taagatgcgc cagcattgca tccagcaacc aggctgcgtg gtatcaacga
    30601 aatgacatta acgacgcata ctctttgaca acatcatctg ccacttctgc tcgcggttaa
    30661 gttccgtgag cggaacggca ggaatacgcg gcgtctttct tacgccgact aatttatccc
    30721 ggctgttatt gcgcaggcgc gcgtaaattt gctcatcgat atcgaccact tttaccagtc
    30781 gctggcactg gcatccccgt ccttccagct tattagggat catcttaact gcgcagctta
    30841 tttctgcgac ttccgacaga atataagtca gggtattaac cgccttgctg tgttcaatat
    30901 cgaaatgaat agagatctct cttgctatca cccaccgatt ttcagccatc atccattcgg
    30961 cgattaacaa gtagggtggt ttttcaacat atccttcaca catacgaact ccagtttcac
    31021 aaattaaaag tctggctatg actcgttttg aagcaattaa acaaggccat attaaaattg
    31081 tggatatatc gatagtatgc aacttcaccg tcgataa
//
LOCUS       DAZPDU010000033        30986 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZPDU010000033 DAZPDU010000000
VERSION     DAZPDU010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30986
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.033"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            59..1423
                     /locus_tag="ACHURS_001374"
     CDS             59..1423
                     /locus_tag="ACHURS_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1237150.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSIVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(1551..2042)
                     /gene="yajQ"
                     /locus_tag="ACHURS_001375"
     CDS             complement(1551..2042)
                     /gene="yajQ"
                     /locus_tag="ACHURS_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFR1237151.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2210..3121
                     /gene="panE"
                     /locus_tag="ACHURS_001376"
     CDS             2210..3121
                     /gene="panE"
                     /locus_tag="ACHURS_001376"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFR1237152.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3084..3674
                     /gene="yajL"
                     /locus_tag="ACHURS_001377"
     CDS             3084..3674
                     /gene="yajL"
                     /locus_tag="ACHURS_001377"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFR1237153.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(3728..5176)
                     /gene="thiI"
                     /locus_tag="ACHURS_001378"
     CDS             complement(3728..5176)
                     /gene="thiI"
                     /locus_tag="ACHURS_001378"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFR1237154.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDEKQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5382..5624
                     /gene="xseB"
                     /locus_tag="ACHURS_001379"
     CDS             5382..5624
                     /gene="xseB"
                     /locus_tag="ACHURS_001379"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFR1237155.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLPPFTPDNE"
     gene            5624..6523
                     /gene="ispA"
                     /locus_tag="ACHURS_001380"
     CDS             5624..6523
                     /gene="ispA"
                     /locus_tag="ACHURS_001380"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFR1237156.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            6548..8410
                     /gene="dxs"
                     /locus_tag="ACHURS_001381"
     CDS             6548..8410
                     /gene="dxs"
                     /locus_tag="ACHURS_001381"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFR1237157.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVTAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            8465..9439
                     /gene="yajO"
                     /locus_tag="ACHURS_001382"
     CDS             8465..9439
                     /gene="yajO"
                     /locus_tag="ACHURS_001382"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFR1237158.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFISMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(9493..10011)
                     /gene="pgpA"
                     /locus_tag="ACHURS_001383"
     CDS             complement(9493..10011)
                     /gene="pgpA"
                     /locus_tag="ACHURS_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFR1237159.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(9989..10966)
                     /gene="thiL"
                     /locus_tag="ACHURS_001384"
     CDS             complement(9989..10966)
                     /gene="thiL"
                     /locus_tag="ACHURS_001384"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFR1237160.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11044..11463)
                     /gene="nusB"
                     /locus_tag="ACHURS_001385"
     CDS             complement(11044..11463)
                     /gene="nusB"
                     /locus_tag="ACHURS_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFR1237161.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(11483..11953)
                     /gene="ribE"
                     /locus_tag="ACHURS_001386"
     CDS             complement(11483..11953)
                     /gene="ribE"
                     /locus_tag="ACHURS_001386"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFR1237162.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12042..13145)
                     /gene="ribD"
                     /locus_tag="ACHURS_001387"
     CDS             complement(12042..13145)
                     /gene="ribD"
                     /locus_tag="ACHURS_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFR1237163.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(13149..13598)
                     /gene="nrdR"
                     /locus_tag="ACHURS_001388"
     CDS             complement(13149..13598)
                     /gene="nrdR"
                     /locus_tag="ACHURS_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFR1237164.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            13749..14288
                     /locus_tag="ACHURS_001389"
     CDS             13749..14288
                     /locus_tag="ACHURS_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFR1237165.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            14587..15471
                     /gene="tsx"
                     /locus_tag="ACHURS_001390"
     CDS             14587..15471
                     /gene="tsx"
                     /locus_tag="ACHURS_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFR1237166.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYDAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(15648..15995)
                     /gene="yajD"
                     /locus_tag="ACHURS_001391"
     CDS             complement(15648..15995)
                     /gene="yajD"
                     /locus_tag="ACHURS_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFR1237167.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(16124..17095)
                     /gene="secF"
                     /locus_tag="ACHURS_001392"
     CDS             complement(16124..17095)
                     /gene="secF"
                     /locus_tag="ACHURS_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFR1237168.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(17106..18953)
                     /gene="secD"
                     /locus_tag="ACHURS_001393"
     CDS             complement(17106..18953)
                     /gene="secD"
                     /locus_tag="ACHURS_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFR1237169.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(18981..19313)
                     /gene="yajC"
                     /locus_tag="ACHURS_001394"
     CDS             complement(18981..19313)
                     /gene="yajC"
                     /locus_tag="ACHURS_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFR1237170.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(19336..20463)
                     /gene="tgt"
                     /locus_tag="ACHURS_001395"
     CDS             complement(19336..20463)
                     /gene="tgt"
                     /locus_tag="ACHURS_001395"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFR1237171.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(20519..21589)
                     /gene="queA"
                     /locus_tag="ACHURS_001396"
     CDS             complement(20519..21589)
                     /gene="queA"
                     /locus_tag="ACHURS_001396"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFR1237172.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            21682..22263
                     /gene="acpH"
                     /locus_tag="ACHURS_001397"
     CDS             21682..22263
                     /gene="acpH"
                     /locus_tag="ACHURS_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFR1237173.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(22268..24085)
                     /gene="malZ"
                     /locus_tag="ACHURS_001398"
     CDS             complement(22268..24085)
                     /gene="malZ"
                     /locus_tag="ACHURS_001398"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFR1237174.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKHRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLGLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGSTEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNAVQWQCKERHGQLTDGILALPAISATVWMN"
     gene            complement(24241..25614)
                     /gene="proY"
                     /locus_tag="ACHURS_001399"
     CDS             complement(24241..25614)
                     /gene="proY"
                     /locus_tag="ACHURS_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFR1237175.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRCLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(25690..27009)
                     /gene="brnQ"
                     /locus_tag="ACHURS_001400"
     CDS             complement(25690..27009)
                     /gene="brnQ"
                     /locus_tag="ACHURS_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFR1237176.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(27416..28711)
                     /gene="phoR"
                     /locus_tag="ACHURS_001401"
     CDS             complement(27416..28711)
                     /gene="phoR"
                     /locus_tag="ACHURS_001401"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFR1237177.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEESGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(28769..29458)
                     /gene="phoB"
                     /locus_tag="ACHURS_001402"
     CDS             complement(28769..29458)
                     /gene="phoB"
                     /locus_tag="ACHURS_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFR1237178.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            29648..30850
                     /gene="sbcD"
                     /locus_tag="ACHURS_001403"
     CDS             29648..30850
                     /gene="sbcD"
                     /locus_tag="ACHURS_001403"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFR1237179.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            30847..>30986
                     /locus_tag="ACHURS_001404"
     CDS             30847..>30986
                     /locus_tag="ACHURS_001404"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR1237180.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLL"
BASE COUNT         7623 a         8408 c         7828 g         7127 t
ORIGIN      
        1 tgtttaccca acatgcgctc cgcactacac tatgtgggca tttttttaag gggtggtaat
       61 gaacgattat aaaatgacgc caggtgagag gcgcgcgacc tggggtttag ggaccgtatt
      121 ctcgttgcgc atgctgggca tgtttatggt tctgccggtt ctgaccacgt acggcatggc
      181 tctgcaaggt gccagcgaag cattaatcgg tattgccatt ggtatttatg gtctgactca
      241 ggccgttttt cagattccgt ttggcctgct ttccgaccgc attggtcgca aaccattaat
      301 tgtcggtggg ctggcggtgt ttgccgccgg tagcgttatc gctgcgcttt ccgactccat
      361 ctggggaatt attctgggcc gggcgctaca aggctctgga gcaattgccg ccgccgttat
      421 ggcgctgctt tccgatctca cgcgcgaaca aaaccgcacc aaagcgatgg cgtttatcgg
      481 cgtgagcttt ggcattacct ttgccattgc gatggtgctt ggcccgatca tcactcacaa
      541 acttgggctg cacgcgctgt tctggatgat cgctattctg gcaacaaccg gcattgcgtt
      601 gaccatttgg gttgtgccca acagtagcac tcacgtactt aatcgtgagt ccggaatggt
      661 gaaaggcagt ttcagtaaag tgctggcgga accgcggctg ctgaaactca actttggcat
      721 tatgtgtctg cacatgcttc tgatgtctac gtttgttgcc ctgcccggac agctagctga
      781 tgcggggttc ccggcggctg aacactggaa ggtctatctg gcgacgatgt taatcgcctt
      841 tggctcgatc gtgcctttca ttatctacgc tgaagttaag cgcaaaatga agcaagtctt
      901 tgtcttctgc gtcgggttga tcgtggttgc ggaaattgtg ttgtggaacg cacaaacgca
      961 gttctggcaa ctggtggtcg gcgtgcagct tttctttgtg gcgtttaatt tgatggaagc
     1021 ccttctgccc tcacttatca gtaaagagtc gccagcaggt tacaaaggta cggcgatggg
     1081 tgtctactcc accagccagt ttcttggcgt ggcgattggc ggttcgctgg gcggctggat
     1141 tgacggcatg tttgacggtc agggggtatt tctcgctggc gcaatgctgg ccgcagtgtg
     1201 gctggcagtc gccagtacca tgaaagaacc gccgtatgtc agcagtttgc gcattgaaat
     1261 cccggcggac attgccgcaa acgaagcgtt aaaagtgcgt ttgctggaaa ctgaaggcgt
     1321 caaagaagtg ttgattgcag aagaagagca ttcagcttat gtgaaaatcg acagcaaagt
     1381 gacaaatcgc tttgaggttg aacaggcaat tcgtcaggca taaaaaacgc ggcgaattgc
     1441 ctgctgcgct acgcttacca ggcctacaaa gttcttgagt tacaaaccgt tgtaggccga
     1501 atcaggcgtt catgccgcat ctgacatgaa caaaacgcac attgtcgcga ttaatcgcgg
     1561 aagtttttga actggaacgg ctggccgaga tcgccaccac gtaccatcgc catgacagcc
     1621 tgcaaatcat cacgagattt gccagtcaca cggatctcat cgccctgaat ttgcgcctgc
     1681 actttcagtt tgctgtcttt aatcatcttg acgattttct tctgggtcgc gctctcaatg
     1741 ccctgtttca gtttcgcttc cacaaaccag gttttaccgc tatgaacgat attttccggt
     1801 acatccagtg aactgccttc aatgccgcgc ttcagcagct tggcacgcag aatatccagc
     1861 aactgattga cctggaaatc ggactcgctc aacactttga tggttttgct ggcgtcgttc
     1921 agctcaaatg aggcttcaac attacggaag tcaaaacggg actccacttc gcggctcgcg
     1981 ttatcgaccg cgttacgtgc ttcctgaaga tcaacttcag agacaatatc gaaagatggc
     2041 atcttttcct ctcccttcat ttttgatgcg aagcataata cccgcaaagt taaataaccg
     2101 acagtcctgc accaggacgt atcccttaat tgtgcatagt cacaacagca tgcacaatta
     2161 agggatagcc ttattgaaca cccggagtgg ttgcgggtga ggaggaacaa tgaaaattac
     2221 cgtattggga tgcggtgcct tagggcaatt atggcttaca gcactttgta aacagggtca
     2281 cgaagttcag ggctggctgc gcgtaccgca accttattgt agtgtgaatc tggttgagac
     2341 tgatggttcg atatttaacg aatcgctgac cgccaacgat cccgattttc ttgccaccag
     2401 cgatctgctc ctggtgacgc tgaaagcatg gcaggtttcc gatgccgtca aaagcctcgc
     2461 gtccacactg cctgtaacca cgccaatact gttaattcac aacggcatgg gcaccatcga
     2521 agagttgcaa aacattcagc agccattact gatgggcacc accacccatg ccgcccgccg
     2581 cgacggcaat gtcattattc atgtggcaaa cggaatcacg catattggtc cggcacggca
     2641 acaggacggc gattacagct atctggcgga tattttgcaa accgtgttgc ctgacgttgc
     2701 ctggcataac aatattcgcg ccgagctgtg gcgcaagctg gccgtcaact gtgtgattaa
     2761 tccactgacc gccatctgga attgcccgaa tggtgaatta cgtcatcatc cgcaagaaat
     2821 tatgcagata tgcgaagaag tcgcggcagt gatcgagcgc gaagggcatc atacttcagc
     2881 agaagatttg cgtgattacg tgatgcaggt gattgatgcc acagcggaaa atatctcgtc
     2941 gatgttgcag gatatccgcg cgctgcgcca caccgaaatc gactatatca atggttttct
     3001 cttacgccgc gcccgcgcgc atgggattgc cgtaccggaa aacacccgcc tgtttgaaat
     3061 ggtaaaaaga aaggagagtg aatatgagcg catcggcact ggtttgcctc gcccctggta
     3121 gtgaagagac tgaagccgtc accactatcg atctgctggt tcgcggcggt atcaaagtca
     3181 ccactgccag cgtcgccagc gatggcaacc tggccattac ctgctcgcgc ggcgtgaagc
     3241 tgctggcgga tgcgccgctg gtcgaggtgg ctgatggcga atatgacgtg atcgtactgc
     3301 ctggtggcat taaaggcgca gagtgttttc gcgatagtac cctgctggtt gaaaccgtta
     3361 aacagttcca ccgttccggg cgtatcgtcg cggctatttg cgccgcgcca gccaccgtgc
     3421 tggtaccgca cgatatcttc ccgattggca atatgaccgg cttcccgacg ctgaaagaca
     3481 aaattcccgc cgaacaatgg caggacaagc gcgtcgtctg ggatgcacgg gtaaaattgc
     3541 tgaccagcca ggggccgggt acggctattg attttggtct gaaaattatc gacctgctgg
     3601 ttgggcgcga gaaagcccat gaagtggcat cacaactggt gatggcggca gggatttata
     3661 attattacga gtagtgtcgg atgcggcaaa cgtcgcatct gaccagatgc gacgtaaaaa
     3721 ccacaaatta tgggcgatac accttcacat tgttaaagcc ctgctcgcgc agatagagcg
     3781 cctgcagacg gctcatcacc ccgcgctcac accacagtag ccaggttctg ttctggtcga
     3841 gatcgccaaa tttggtgctc agtttataga acggcagaga aaccacgtcg ataccttcaa
     3901 ccttcagcgg cttatcttcc tgctcatcga cagaacggat atcgaggatc acgtcgttcg
     3961 ggccaaagcc attgacggtt tccacttcca ccacttcctg ctcggtctgc tgggcgattt
     4021 cgcggatatc aacgttattc gcttcctcaa ccactttatc gagaatactg aagtcgaact
     4081 tctcttcttc cgcttcaatc ttcgatttaa ccgctttcac cgtcgggctt ttggagatca
     4141 cgccacaata ttctggcatc gtgcgagcaa agtcttcagt gccaatctgg cgggccaggt
     4201 tgatgatgtg ctctttgtcg taagaaatca gcggacgcag gatcagcgta tcagagacgt
     4261 tatcaatcag gcgcaggttg gtcagcgtct gactggacac ctggccgagc gcttcgccgg
     4321 tgaccagcgc ctgtacgccg taacgttcag ccactttaga cgcggcacgc accatcatac
     4381 gtttgaggat aacgcccatc tgaccgtcgt cgattttctc gagaatttcc ccgacgaccg
     4441 gttcgaaatt aatggcgaca aaacgcacgc ggtgggagct gccaaagcgg ttccacagat
     4501 aatgcgccac ctgacgcacg ccaatttcat gcgctgcgcc gccgaggtta aagaagcagt
     4561 aatgcacgcg gcagccgcga cgcatcaaca tataactgga aacaccggag tcgaaaccac
     4621 cggaaatgag cgacagcaca tcttcctggg tgccaatcgg gaaaccgcca ataccttcgt
     4681 agcggccttt aatcagcaga agacgatcgt cttccacttc cagatggaca gttacatccg
     4741 ggttagtcag cttcacgcgc gcggattcaa tatgctgatt taaaccgccg ccgacgtaac
     4801 gttccacatc aatcgagcta aaatcatgtt tgccacggcg cttcacgcgt acgcagaagg
     4861 ttttgccttc cagctgatcg cgatattgaa ccaacgcttt ctcgaaaata tcgtgcatgt
     4921 cggtaaacgg cacgtcttcg acttcgagaa tatggtggat acccggaata cgggtcaggg
     4981 cgtcgcgaat agccagacgc tgtttttcat cttttgcgcg aacttcgatg ttatcccagt
     5041 ggcggacgac agcgagcgtc tcatcatagt gctttaaaac gttacgaatg ttcccggtaa
     5101 ggatttttat aaagcgcaag cgcacagatt ggcttttgat ggtgatttcc gggaacaatt
     5161 taatgataaa cttcatggcg gcaatggttc gttggcaagc cttaagcgac ttgtataggg
     5221 aaaaatacag cagcccacac ctgcggctgc atccaggcgc ggaagtatac cactaacatc
     5281 gctttgctgc gcacatcacc ttaccattgc gcgttatttg ctatttgccc tgagtccgtt
     5341 accatgacgg ggcgaaaaat attgagagtc agacattcat tatgccgaag aaaaatgagg
     5401 cgcccgccag ctttgaaaag gcgctgagcg agctggaaca gattgtaacc cgtctggaaa
     5461 gtggcgacct gccgctggaa gaggcgctga acgagttcga acgcggcgtg cagctggcac
     5521 gtcaggggca ggccaaatta caacaagccg aacagcgcgt acaaattctg ctgtctgaca
     5581 atgaagacgc ctctctaccc ccttttacac cggacaatga gtaatggact ttccgcagca
     5641 acttgaagcc tgcgttaagc aggccaacca ggcgctgagc cgttttatcg ccccactgcc
     5701 ctttcagaac actcccgtgg tcgaaaccat gcagtatggc gcattattag gtggtaagcg
     5761 cctgcgacct tttctggttt atgccaccgg tcatatgttc ggcgttagca caaacacgct
     5821 ggacgcaccc gctgccgccg ttgagtgtat ccacgcttac tcattaattc atgatgattt
     5881 accggcgatg gatgatgacg atctgcgtcg cggtttgccg acctgccatg tgaagtttgg
     5941 cgaagcaaac gcgattctcg ctggcgacgc tttacaaacg ctggcgttct cgattttaag
     6001 cgatgccgat atgccggaag tgtcggaccg cgacagaatt tcgatgattt cagaactggc
     6061 aagcgccagc ggtattgccg gaatgtgcgg tggtcaggca ttagatttag atgcggaagg
     6121 caaacacgta cctctggacg cactcgagcg tattcatcgt cataaaaccg gcgcattgat
     6181 tcgcgccgcc gttcgccttg gtgcattaag cgccggagat aaagggcgtc gtgctctgcc
     6241 agtactcgac aagtacgcag agagcatcgg ccttgccttc caggttcagg atgacatcct
     6301 ggatgtggtg ggagatactg caacgttggg aaaacgccag ggtgccgacc agcaacttgg
     6361 taaaagtacc taccctgcac ttctgggtct tgagcaagcc cggaagaaag cccgggatct
     6421 gatcgacgat gcccgccagt cgctgaaaca actggctgaa caatcactcg atacctcggc
     6481 actggaagcg ctagcggact acatcatcca gcgtaataaa taaacaataa gtattaatag
     6541 gcccctgatg agttttgata ttgccaaata cccgaccctg gcactggtcg actccaccca
     6601 ggagttacga ctgttgccga aagagagttt accgaaactc tgcgacgaac tgcgccgcta
     6661 tttactcgac agcgtgagcc gttccagcgg gcacttcgcc tccgggctgg gcacggtcga
     6721 actgaccgtg gcgctgcact atgtctacaa caccccgttt gaccaattga tttgggatgt
     6781 ggggcatcag gcttatccgc ataaaatttt gaccggacgc cgcgacaaaa tcggcaccat
     6841 ccgtcagaaa ggcggtctgc atccgttccc gtggcgcggc gaaagcgaat atgacgtatt
     6901 aagcgtcggg cattcatcaa cctccatcag tgccggaatt ggtattgcgg ttactgccga
     6961 gaaagaaggc aaaaatcgcc gcaccgtctg tgtcattggc gatggcgcga ttaccgctgg
     7021 catggcgttt gaagcgatga atcacgcggg cgatatccgt cctgatatgc tggtggtcct
     7081 caacgacaat gaaatgtcga tttccgaaaa tgtcggcgcg ctcaataacc atctggcaca
     7141 gctgctttcc ggtaagcttt actcttcgct gcgcgaaggc gggaaaaaag ttttctctgg
     7201 cgttccgcca attaaagagc tgctcaaacg taccgaagaa catattaaag gcatggtagt
     7261 gcctggcacg ttgtttgaag agctgggctt taactacatc ggcccggttg acggtcacga
     7321 tgtgctgggg cttatcacca cgctgaagaa catgcgcgac ctgaaaggcc cgcagttcct
     7381 gcatatcatg accaaaaaag gtcgtggtta tgaaccggca gaaaaagacc ccatcacttt
     7441 ccacgccgtg cctaaatttg atccctccag cggttgtttg ccgaaaagta gcggcggttt
     7501 gccgagctat tcaaaaatct ttggcgactg gttgtgcgaa acggcagcga aagacaacaa
     7561 gctgatggcg attactccgg cgatgcgtga aggttccggc atggtcgagt tttcacgtaa
     7621 attcccggat cgttacttcg acgtggcaat cgccgagcaa cacgcggtga cctttgccgc
     7681 cggtctggcg attggtgggt acaaacccat tgtcgcgatt tactccactt tcctgcaacg
     7741 cgcctatgat caggtgctgc atgacgtggc gattcaaaag ctcccggtcc tgttcgccat
     7801 cgaccgcgcg ggcattgttg gtgctgacgg tcaaacccat cagggcgctt ttgacctctc
     7861 ttacctgcgc tgcataccgg aaatggtcat tatgaccccg agcgatgaaa acgaatgtcg
     7921 ccagatgctc tataccggct atcactataa cgacggcccg tccgcggtgc gctacccgcg
     7981 cggtaacgcg gttggcgtgg aactgacgcc gctggaaaaa ctgccaattg gcaaaggcat
     8041 tgtgaagcgt cgtggcgaga aactggcgat ccttaacttt ggtacgctga tgccagacgc
     8101 ggcgaaagtc gctgaatcgc tgaacgctac gctggtcgat atgcgttttg tgaaaccgct
     8161 tgatgaagcg ttaattctgg aaatggccgc cagccatgaa gcgctggtca ccgtagaaga
     8221 aaacgccatt atgggcggcg caggcagcgg cgtgaacgaa gtgctaatgg cccatcgtaa
     8281 accagtaccc gtgctgaaca ttggcctgcc tgacttcttt attccacaag gaactcagga
     8341 agaaatgcgc gccgaactcg gcctcgatgc cgccggtatg gaagccaaaa tcaaggcctg
     8401 gctggcataa tccctactcc actcctgcta tgcttaagaa attattcatg gcaggagtgg
     8461 cagcatgcaa tacaacccct taggaaaaac cgaccttcgc gtttcccgac tttgcctcgg
     8521 ctgtatgacc tttggcgagc cagatcgtgg taatcacgca tggacactgc cggaagagag
     8581 tagccgtccc atcattaaac gcgcgctgga aggcggcata aatttctttg ataccgccaa
     8641 tagctattcc gatggcagca gcgaagagat cgttggtcgc gcactgcggg atttcgcccg
     8701 tcgtgaagac gtggtcgttg cgacgaaagt gttccatcgc gttggtgatt taccggaagg
     8761 attatcccgt gcacaaattt tgcgctctat cgacgacagc ctgcgccgtc tcggcatgga
     8821 ttatgtcgat atcctgcaaa ttcatcgctg ggattacaac acgccgatcg aagagacgct
     8881 ggaagccctg aacgacgtgg taaaagccgg gaaagcgcgt tatatcggcg cgtcatccat
     8941 gcacgcttcg cagtttgctc aggccctgaa gctacaaaaa cagcacggct gggcgcagtt
     9001 tatcagtatg caggatcact acaatctgat ttatcgcgaa gaagagcgcg agatgctgcc
     9061 tctgtgttat caggagggcg tggcggtgat tccgtggagc ccgctggcgc gggggcgact
     9121 gacgcgtccg tggggagaaa ctaccgcacg actggtgtct gatgaggtgg ggaaaaatct
     9181 ctataaagaa agcgatgaaa atgacgcgca gatcgcagag cggttaacgg gcgtcagtga
     9241 agaactcggt gcaacacgag cacaagttgc gctggcctgg ttattgagta aaccgggcat
     9301 tgccgcaccg attatcggta catcgcggga agaacagctt gatgagctgc tgaacgcggt
     9361 ggatatcact ttgaagccag aacagattgc cgaactggaa acgccgtata aaccgcatgc
     9421 ggtcgtagga tttaaataag agtccaggcc tgatgagacg tgacaagcgt cacatcaggc
     9481 atcggtgcac aactacgaca gaatacccag cggccagtga tgaccgataa agtacaggat
     9541 gcctgcggaa atcaccccgg cgacaatatc gtcgatcatg atccccatgc cgccatgtac
     9601 attgcgatca aaccagcgga tcggccacgg cttccacata tccagaatac ggaaaatcac
     9661 aaacccggcg gcaacccact gccagtcatt ggtcggcagc gccatgagcg tgatccacat
     9721 accaataaat tcgtcccaga caatgctgcc gtgatcgtgc acgcccatat ctttcgccgt
     9781 ttgatgacaa agatagacgc cgatacagat ccccaacatc accaccagcg agtagagctg
     9841 ccagggtaaa aaggtcatca gataccagaa cggaatcgct gccagcgatc ccatcgtgcc
     9901 aggaacgatt gggcttaatc cacttccgaa tccgacagca agcagatgcc acggattact
     9961 catcttcagg cgacttttcg cgacatcttt atggcgtggc aaaatggtca tatcctttcc
    10021 agtcaaacgt gacaggttcg ccgtcacgaa taaaacaaag cccttcgata tcggcggtca
    10081 tttgcccgat acaggtaaac ggtacgccaa ggtgtccgag agcaacatcc agcgcgccac
    10141 ggttcagttc cggcaccgtg aaacacaact cgtaatcttc accgccagag agcgcccagc
    10201 gcagcgcctg ttccggttca acatggcgag aaagcgcatc agaaaacggc agcaatgcta
    10261 ggtcaatacg tgcgccgcag tcgctggctt tcacgatatg cccgagatcg gaaatcagac
    10321 cgtcagagag atcgatagct gaatttgcca gatcgcgcag tgcctgcccc tgtaaaatac
    10381 gcggcgatgg acggagatga cgtttgatca agtagtcagc atctttagca tcggcaacct
    10441 gcaaacggtt ttgcaaaatc gccagcccgg cggcgctatc gcccggtgta ccggtcacat
    10501 agatccagtc acccggtttc gccccagagc gcgttaaggc tcgtcccatc ggaacaaagc
    10561 cgtggatacc caacgtcatt gataatggcc cacgcgtggt atcgccgcca atgagttgca
    10621 tatcgtaata attgagaaga tcaaacaaac tgtcgctgaa ggactcaagc cacgcttcgt
    10681 ctacgtccgg taaggttaat gccagcgtca gccaggccgg atcggccccc atcgctgcca
    10741 gatcgcttag gttcaccgcc agtgctttat aagccagatc agcaggatcg atatcaggga
    10801 ggaaatggtt acccgccacc agcgtatcag tgctgatcgc cagggtctgt ttctcgggga
    10861 tattgagaag tgcgcaatcg tcgccgatgc ccagttcgac atcaagacga gaacttctta
    10921 cacggtcaaa ataacgggca atcagggaga actcgccaca tgccatacgt tatgcctcag
    10981 caggtaaaaa agaaaaggcc gacgatcatg gaacggtctt ccgtgaatct accggcctgg
    11041 atatcacttt ttgttagggc gaatcacagg tgctgcttta tcgagtacgc cgttgacgaa
    11101 cttatggctg tcttctgcgc cgaacgattt cgccagttcg atcgcttcgt taatggccac
    11161 tttgtatggc acatcgctac gtttagacag ttcgtacagc gcaatgcgca gtactgcttt
    11221 ttctacctgt cccagttctt ccagcaggcg ggacaggtat ggcttcatca gtccgtcgag
    11281 gtatgcggta ttagtcgcca ccccggccag cagctcacgg aagtacagga cgtcaacgtc
    11341 ttttacatcc tgttcagcca ggaactggta ttcaacatca gcgatgtcgt tctgggacaa
    11401 ctgccaggag tagagcgcct ggacggcaca ctcacgagcg cggcgacgag cagcaggttt
    11461 cacggatttc cccttactaa tttcaggcct tgatggcttt caatacatta atcatttcaa
    11521 gcgcggtcag tgcagcttct gcacctttgt tgccagcttt ggtgccagca cgttcgatcg
    11581 cttgttcaat gctttcagtg gtcagaaccc caaaagcaac cggaatttcg ctgtcctggg
    11641 caacatgcgc caggccgttg cttgcaccac cagcgacata ttcaaagtgg gcagtgccac
    11701 cacgaataac cgtacccagc gcaatcaccg cgtcgtattt accggtttta gccagtgcac
    11761 ccgccgccag cggcagctca taggcaccag gcacccaaac aacggtaatg ttttcatctt
    11821 ttacctgacc gatacgtttc agtgcgtcaa ttgcaccttc cagcaggctg tcattgataa
    11881 agttgttgaa acgcgcgatg gtgatggcga cgcgagcgtc cggggtagca acgttagctt
    11941 caataatgtt cattttcttc cttcgggttc gagtatggcc ccgcaggggg gcggatttta
    12001 gcataatatt tcgtgcgctg cttccctttc gagccgggag atcatgcacc cactaaatgc
    12061 aggcaaacat ccgggcctac atgacgtatc tctttgaatt taaattgggg ggcgtcggct
    12121 aatttctcaa gccctggcag cgtgcataat ccgcgggcgt cgctgcctaa tagtttaggt
    12181 gcgatataga caatcagctc atcgactaaa cccgcctgca gcaatgcgcc agcgagcgtt
    12241 ggccccgctt ccacccagat gctgttaatt tgctgtttac ccagttgcat catcagtaca
    12301 accagatcca gatgaccttt atgctctgga atcagcaagg tacgcaccgt ttccggccac
    12361 tcactagaat cttcctgcgt acgcgcgaac caggtttcgc cgggctgctg cacaatgcga
    12421 tgttccggcg tcacgcgatt ttggctatca atcacaatac gtactggctg gcggagattt
    12481 tgttgcgggt agagcgcctg agtttgttca tccagttcag accaacgcac tgttaaggct
    12541 ggatcatccg ccagcaccgt ggcgctgctg gttaaaatgg catgactttg tgcgcgctgt
    12601 cgttgtacgt cgcgccgcgc ctgaggcgaa gtgatccact ggctttcgcc gctcgccatc
    12661 gccgtgcggc catcaagcga tgcgccaagt tttaactgaa tataaggaaa gccggtgcgc
    12721 atccgcttga gaaagccttt attcaattgc tcggcttcac tcatcatcag cccgtggctg
    12781 acgtcaatgc cagcctgttg cagacggtaa agtccacgcc cagcgacctg cgggttagga
    12841 tcctgcatcg ctgcgaccac gcgcgctacg ccagcggcga tgagtgcgtc acagcacggt
    12901 ggcgtacggc catgatggct acagggttcg agtgtcacat acgccgttgc gcctttggct
    12961 ttttcacccg ccatacgcaa cgcgtgtact tcggcatgtg gttcacccgc tcgttggtgg
    13021 taaccttcac cgacaatttc gccatctttg acaatgacgc acccgacatt cgggttgggg
    13081 tgggtggtaa aacgtcctcg ttgcgccagc tttagcgccc gcgccatgta atactcgtcc
    13141 tgcacggctt agtcctccag gcgcgcgatc tcttcgccaa attctttgat atcttcgaaa
    13201 ctgcggtaga cagaggcaaa acggatatag gcgactttat cgagcttttt caattgctcc
    13261 atcaccagat tgccaatcat cttgctcggt acttcgcgct caccggtggc gcgcagctgc
    13321 gatttaatat gattgattgc catttcaacg tcatcggaac tcaccggacg tttttccagc
    13381 gcccgcagca ttccgctacg caatttctct tcattaaacg gttcacgcac gtcgttgctt
    13441 tttacaacac gcggcataac cagctccgcc acttcaaagg tggtgaaacg ttcattacac
    13501 accagacact gccgacggcg gcgtacggat gaaccctcgc ccacgagacg agagtcaatt
    13561 accttagtgt ccacggcgaa acagaatggg caatgcatac ggcgtcctga ccaggtggtt
    13621 aacggaaatc tattttaccc tgaactgacg atacaacaaa ggcgcaacgc ttttgagata
    13681 aaggatcgat gctttcgctg cttttaccct ctaccattag gctaattgcg acgagaaagg
    13741 gacgacctat gaatactaac gtttttcgac tgctcctgct gggaagcctg ttcagcctta
    13801 gcgcctgtgt gcagcaaagt gaagtgcgac agatgaaaca cagcgtcagc acgctgaacc
    13861 aggagatgac gcaactcaat caagaaacag tcaaaatcac tcagcaaaac aggctgaatg
    13921 caaaatccag cagtggggtt taccttctgc ctggagcgaa aacaccggca agactggaaa
    13981 gccagatcgg tactttacgt atgtcgctgg tgaatattac gcctgataca gatggcacca
    14041 cccttacact acgtattcag ggtgagtcga atgatccctt gcccgcattc agcggaactg
    14101 ttgaatatgg gcaaattcag ggaacaatag acaactttca ggaaatcaat gtgcagaatc
    14161 aattgattaa tgcccctgcc agcgtcctcg cccccagcga tgttgatatt ccgttacaat
    14221 taaaggggat ttctgtggat cagttaggct ttgtacgtat ccacgacatt caacctgtta
    14281 tgcagtaaac gtatttcggg acgattttgt gcgtcccgca acatctttcc ccgtcatttt
    14341 gttactctgc ttacatcacc tggattgata gtaaaagttt gcaacaaggg cgaaagtcag
    14401 tacaatcccc gcccgaatgt gtgtaaacgt gaacgcaatc gattacgtaa atgatagaac
    14461 tgtgaaacga aacatatttt tgtgagcaat gatttttata ataggctcct ctgtatacga
    14521 aatatttaga aacgcaattt gcgccttttt cactcccgca agggattttc aaacagtggc
    14581 atacatatga aaaaaacatt actggcagcc ggtgcggtac tggcgctctc ttcgtctttt
    14641 actgtcaacg cagctgaaaa cgacaaaccg cagtatcttt ccgactggtg gcaccagagc
    14701 gttaacgttg tcggaagcta tcacacccgt ttcggaccgc agatccgcaa cgatacctac
    14761 cttgagtacg aagcattcgc taaaaaagac tggttcgact tctatggtta tgcggatgcg
    14821 ccggtattct tcggcggtaa ctccgatgct aaaggtatct ggaaccacgg ttctccgctg
    14881 tttatggaaa tcgaaccacg tttctccatc gacaagctga ccaatactga ccttagcttc
    14941 ggtccgttca aagagtggta cttcgcgaac aactacattt acgacatggg tcgtaataaa
    15001 gatggtcgcc agagcacctg gtacatgggt ctgggtaccg acatcgatac tggtctgccg
    15061 atgagcctgt ccatgaacgt ctatgcgaaa taccagtggc agaactatga cgcagcgaac
    15121 gaaaacgagt gggacggtta ccgtttcaaa attaaatact ttgtgccgat taccgatctg
    15181 tggggcggtc agctgagcta catcggcttc accaacttcg actggggttc cgatttaggg
    15241 gatgacagcg gtaacgcaat caacggtatt aagacccgta ctaataactc tatcgcttcc
    15301 agccatattc tggctctgaa ctacgatcac tggcactact ctgtcgtagc tcgttactgg
    15361 cacgacggtg gtcagtggaa cgacgatgca gaactgaact tcggcaacgg caacttcaac
    15421 gttcgctcta ccggctgggg tggttacctg gtagtaggtt acaacttctg attatgaaaa
    15481 tgccgggatt tattcccggc atttctgatt gttaacaacg tgtgctttgt tcatgccgga
    15541 tgcggcgtaa acgctttgtc cggcccacaa aagcacgtaa attcaatata ttgctgaaat
    15601 aacgtaggcc tgataagcgt agcgcatcag gcaattttac gttttaatca cttcttcttg
    15661 ttcatcatcg ctttcaggtc agcgaatggg ttgtacttcg cttcaccgac atctttctgc
    15721 gcatcttccc ctgcgataac ggtcgtacca tactgatccg cttcggtata tttcgaatgc
    15781 tcatgatcgt ggcaatagag acaaagcaat tcccagttac tgccatcttc cgggttattg
    15841 gtatggtcgt gatcaatgtg gtgaacggta agttcacgca ggttggaata aacaaactcg
    15901 cgggaacagc gaccgcagac ccacggatag atttttaatg ctttttcgcg atagccgctt
    15961 tctaaccgcg catagttttt tgggatgata gccatgaaaa gttacctgga tgaaatgtag
    16021 ggttacgcgc agtttcccaa gatgcgggga ttttcgcaat ctccatacat caaaaaaaat
    16081 cccgatcttc tgaccgggat tttcaacatc aacgggaact tgattacggc agaattgacg
    16141 gctgatccgc cccttctttt tccactttct gctgcaacat gtgttcgcgc ttcataccca
    16201 gtttcagagc caacgcagat gctacgtaga tggaagatgc agtgccgatg gaaacaccga
    16261 taagcatggt cagcgagaag ccttccagta ccggaccacc gaagagatac agcatcagga
    16321 taaccatcaa ggtagtaccg gatgtgatca aggtacggtg cagcgtctgg gtcaaggaca
    16381 cgttaaagat ttcgtaaggc gtaccgcgac ggatcttgcg gaagttttca cgaattcggt
    16441 ccgataccac gatactgtcg ttaagcgagt aaccgataac cgacatcaac gatgccacaa
    16501 tggtcaggtc aatctcgata tggaataacg acaaaatacc cagcgtaatg ataacgtcgt
    16561 gcgccagcgc aataaccacc cctgccgcca gtcgccactc aaagcggaaa cctacgtaca
    16621 cgaggataga cagcagcgct gccatcaacg ccatcgcacc ggtttgcgca aggtctgccc
    16681 ccacgctcgg accgacgaac tcaatacgct tcactgctgc attctgattg gtggattcgt
    16741 taatcacctt cagaacctgg ctgcccagca cctgaccgcc ggtttcgcct tcagcaggcg
    16801 gcatacggac catgatgtcg tggctgctac caaagttttg cagcattggc tcttcaaaac
    16861 cggctttttg caatgcatca cgcattacgt caatttcagc cggtttttcg agcgtaattt
    16921 cgataaccgt accaccggtg aaatccagcc cccagttaaa gccgcgcacg cccataataa
    16981 cgatagcagc gattaacagc agaccagaga tgccgaaagc ccagtagtcc cagcgcataa
    17041 agtcatagac tttacggccg tggtttagtt gttcaacagt atattcctgt gccacatcgc
    17101 actcctcaga ttgacagctt cttgacgcgc ttgccgccat atagcaggtt tacgatggca
    17161 cgcgtaccga caatcgcggt aaacatcgac gtcgccacac cgataccggt agtaatcgcg
    17221 aaccctttaa ttgccccggt acccactgcg tacaggatga tgactttaat cagcgtggtg
    17281 atgttcgcat cgaagataga actgaatgcg ccacgataac cttcatcaat tgcctgttga
    17341 acagtacgtc cgttgctcaa ctcttcttta atacgttcgt tgatcagtac gttcgcatcg
    17401 accgctaccg caagggttaa gacgataccc gcgatacctg gcatactcag tgttgcgcct
    17461 ggcaacagcg acataatacc gacgatcaag atcaagttgg caatcagagc actggtcgca
    17521 atcagaccaa acttcttata gaagatgatc atgaacagaa tagaaaccag cagaccggca
    17581 aggcaagcct ccagcccctg ctcaatgttc tgcataccca gggtcgggcc aattgtacgt
    17641 tcttcaacaa tctgaatcgg cgcgatcaac gcaccggcac gcagcagcag tgacagctga
    17701 cgggcttcgt tcgggttgtt gatgccggtg atacggaagc tgttacccag acgagactgg
    17761 atgttggcga tgttaatcac ctcttcctgt ttcaccagaa ccgcacgacc atttgcatct
    17821 ttcttaccgc tgtctttgta ctccacaaac agggttgcca tcggtttgcc gatgttgtcc
    17881 ttggtgaagt tagacataat gttgccacca gcgctatcga gcgagatgtt aacctgcggc
    17941 tggttgtatt cgtcctggct ggaagtggag tcggtgatat ggtcaccggt cagaattacg
    18001 cgtttgtaca gcacaaccgg ctgaccttcg cgggtctgtt tcacttcaga gtcgcccggt
    18061 acgcgaccgg atgccgcagc ggcctgatca acattggtgt ttaccagacg gaattccagc
    18121 gttgccgtcg cgcccagaat ctctttcgca cgcgcagtgt cctgaatacc aggcagttca
    18181 acaacgatac ggtcagcacc ctgacgctga accaccggct ccgccacgcc aagttggttt
    18241 acacggttac gcaggatatt aatgttctgc tgcaccgcat attcacgcgc ttcactcaga
    18301 cgagcatcgc tcattaccgc acgcagctgg ttgctgccct ggctgctaat caccaggtcc
    18361 ggatggcgct tgctcagata cgcaatggct tcatcacgag ctttagcatc gcggaaagtg
    18421 atgctcaggc cgtagttgtt ttctttacga acagtggtat acgggatgcc cttttcgcgc
    18481 aggtcactgc gtaggctatc gatattttgt tcctggagtt tgccaagcgc ggtgtccata
    18541 tccacttcca tcaggaagtg aacgccgcca cgcaggtcaa ggccgagctt catcggctca
    18601 gcatgaatag ctgccagcca gcgcggcgtt gccggggcaa ggttaagcgc cacgacgtat
    18661 ttgtcaccca taacgcccat taatgcttca cgagcgcgca actgggtgtc agtggagtcg
    18721 aagcgcgcaa gaatagcgcc ctcttccagt gccacagact tagcagttat tttttcttct
    18781 tgtaacgttt tctggacctg gatcagcgtt tgctcactgg cggcgactcc gcgcgcacca
    18841 gtgatctgaa cagccggatc ctcaccaaac aggttgggaa gcgcatacag cagaccgatg
    18901 acaatcacca cgatcagcat gacgtacttc cacaaaggat aacggtttaa cacggcaatt
    18961 cccttaggga aaaattttaa ttacagcgcc ttcatggtgc ctttcggcag gacggcagct
    19021 acgaagtcac gtttaataac tacttcagtg gtgtcattca gcgcgatagc aatgtagccg
    19081 ttttccgcta ctttggttac gcgaccaacc aggccaccgt tcgtcagaac ttcatcacct
    19141 ttggcaatgg agtccatcag ctttttgtgt tctttggtgc gcttctgctg tggacgcagg
    19201 atcatgaaat agaaaatcag accgaatacc accagcatca aaatcaaaga catcgggcta
    19261 ccttgcgccg gtgcacccgt tgccgctacc gcatcagaaa taaaaaagct cattaaattt
    19321 ccctcattat taatattaat caacgttcaa aggtggtact tctcgcccct gacgctggta
    19381 aaaatcagtt acgaagctct ctaatttacc ctcttcaata gccttgcgta aacccgccat
    19441 caaacgctgg tagtaacgaa ggttatgaat ggtgttgagt cgcgcgccta atatttcgtt
    19501 gcaacggtca agatgatgca agtaagcgcg tgaataattg cgacaggtgt agcaatcaca
    19561 ctcaggatca agtgggccag tatcgctctt atacttcgca ttgcggattt tcaccacgcc
    19621 atcggtcacg aacaaatgac catttcgggc gttgcgggtt ggcattacgc agtcaaacat
    19681 atcgatacca cgacgtacgc cttcaaccag gtcttctggt ttaccaacgc ccatcaggta
    19741 acgcggtttg tctgccggaa tttgcgggca tacatgctcc agaatgcggt gcatatctgc
    19801 tttcggctca cccacagcca gaccgccgac agcgtagcca tcaaaaccga tatctaccag
    19861 acctttaaca gaaatatcac gtaaatcttc gtaaacgctg ccctgaatga taccaaacag
    19921 cgcatttttg tttccgagac tgtcaaaacg ctcacggcta cgcttcgccc aacgcagaga
    19981 catctccatg gagcgttttg cgtaatccca gtcagcagga tacggcgtac actcatcaaa
    20041 gatcatgacg atatccgaac caagatcgta ctgaatctcc attgattttt caggatcgag
    20101 gaaaatcgga tcgccgttga tcgggttacg gaagtgcacg ccctgttcgg tgattttacg
    20161 aatatcgcca aggctgaaga cctggaagcc gccggagtcg gtgaggatcg gccccttcca
    20221 ctgcataaaa tcgtgcagat cgccgtgcag tttcatgatt tcctggcccg ggcgcagcca
    20281 caggtggaag gtgttgccga ggataatttg cgcgccagtg gcttcaactt cttccggcgt
    20341 catccctttt acggtgccgt aggtgccaac aggcataaaa caaggcgttt ccactacgcc
    20401 acgatcaaag accaggcggc cacggcgtgc gcgaccgtcg gtggtgtcca gttcaaattt
    20461 cattttttct ccactacgtc agaaaaacag tccaacgttt taaaccagcg ccacggaatt
    20521 actccccgac gcgctcatta attgcctgcg gattgtacgt gataaacatc gcatcaccgt
    20581 aactaaaaaa gcgatatttc tcttctaccg ctgctttata ggcgttcatg gtgtgttgat
    20641 aaccggcaaa ggccgaaacc agcataatca gcgtcgattc cggcaggtgg aagtttgtca
    20701 ccagcgcatc gaccactttg tactggaagc ccggatagat aaagatttgg gtatcgtcaa
    20761 agaacggttc gataagatcg tttttcgccg cctgagccgc gctttccagc gaacgtactg
    20821 aggtggtgcc aaccgcaatc acccggttac cgcgcgcttt cgccgccagt accgcgtcta
    20881 ccacatcctg cggcacttca gcgtattccg agtgcatgat gtgatcttca atggtgtcga
    20941 cgcgcaccgg ctggaaggtg cccgcaccaa cgtgcaacgt cacaaacgcc atctccacgc
    21001 ctttggcgcg taatttttcc agcaaaggtt cgtcaaaatg cagacctgcg gtcggggctg
    21061 caaccgcgcc cggtttttcg ctataaacgg tttgataaag ttcgcggtca gcgtcttcgt
    21121 ccggacggtc gatatacggc ggcagcggca tatggccgat gctgttgaga atatccagca
    21181 ccgagcgttc atcattaaat tcgacttcaa acagtgcgcc gtggcgcgcg gtcattgttg
    21241 cgttaatact ttcgtcatcg cccagcagca gttctgcgcc aggttttggc gctttcgagg
    21301 cgcgaatatg cgcaagaatg cgtttgtcgt cgagcatccg ttcaaccagc acttcaatct
    21361 tgccgccgct ggctttacgc ccaaacaggc gcgccgggat cacgcgggta ttattaaaaa
    21421 ccagaagatc gccggggttg agcttatcaa gtaaatcggt gaaagtaccg tgcgtcagcg
    21481 cgcccgtcgg cccgtccagc gacagtaaac gacagctact gcgttcaggc atgggatagt
    21541 gggcaatcag ggattcgggc aattcaaagg agaaatcggt aacgcgcatg acactgactc
    21601 agactaaaat aagaggcggg tagtctagtg ccggggcgct ctccctgcaa cctttacccc
    21661 cttttggata gaatataatc gatgaatttt ttagctcacc tgcatttagc ccatctcgcg
    21721 gaaagctcgc tttccggcaa tttactggct gatttcgtac gcggaaatcc tgaagaaagt
    21781 tttccgcccg acgtcgtggc tggcattcat atgcatcgac gtatcgacgt attaaccgac
    21841 aatctgccgg aagtccgcga agcacgggaa tggtttcgta gtgaaactcg ccgcgttgcg
    21901 cctattacgc tggatgtcat gtgggatcac tttctttccc gccactggtc gcagctgtcg
    21961 ccggactttc cgctacagga atttgtctgt tatgcccgcg agcaagtgat gacgattttg
    22021 ccggactcac cgccacgttt tatcaatctg aacaattact tgtggtcaga gcagtggctg
    22081 gtgcgctatc gcgatatgga tttcatccag aacgtgttaa acggcatggc aagccgccgc
    22141 ccacgtctgg atgccctgcg tgactcctgg tacgatttag acgctcatta tgacgccctc
    22201 gaaacccgct tctggcagtt ttatccgcgg atgatggcgc aggcgtcaca caaggcgtta
    22261 taaaacgtta gttcatccat accgtagccg aaatggcagg caaagccaga atcccgtcag
    22321 tcagttgccc atgcctttct ttgcattgcc attgcacggc attgagcaac ggtgacgcgg
    22381 gtagcacgac ttcacaagct tcgccacggt tgattgccac cagtacacgc tgctgattca
    22441 gcacgcggac aaataccacc acgttatctt ccgcatacag cacctgacag ccgccatgac
    22501 gtagcgcctg acttttctta cgcagcgcaa tcattcgctg gtacagcgcg aataacgccg
    22561 tatcctgctt ttccacctgc caggggaacg gtttacggca aaacggatcg tttttgccat
    22621 ccagtcctac ttcatcaccg taataaatgc acggtacacc aggccaggtg aacagccaga
    22681 ccaccgccag cggcaggcgc gcaatatccc gaccgagcag cgttttaaat cgcgcagtat
    22741 cgtggctgtc gagctgatta aacatacgta attgttgttg atgagaaagc cctgcgcggt
    22801 aattatccat ccaggccata caggtttggg catcaatttg ctgcggatcg taagagatat
    22861 cggtattggc aagaaatccc cacaacggga atgtgaagcc acgatagttc atggcggcat
    22921 cttccacatc ggcctgtaac cattgccgtg catcgccaaa atgttcgccg acaatatacg
    22981 cttccggctg ggtttctttc gccgcttcgg tgctcccagc aacgtgctgc atattattgc
    23041 gcgccccacc cgcctcccct agcatatgca ccacatccag ccgccagccg tccatactcc
    23101 acggcgcttt cagccaatgg cggacaatac tgtcttcccc gcgataaatt tcattcacca
    23161 gactttccga ctgataatcc agcttcggca agctggcata gccaagccag tcgagcgccg
    23221 tgccatcatc actaaacgag taccagtcgc gccagggcga ttcagggttg tgacaagctc
    23281 cccccgtgcc acgattgtgc ctgtcaaacc aggcatggga atcgccactg tggttaaaca
    23341 cgccatccag ccccagccgc attcccagct gctgcgtatt gtggcgtaag cgcagcaacg
    23401 ccccatcacc gccaaactgc ggatcgacat ggcgataatc ctcggtatcg tatttatgta
    23461 cgctgggagc tttaaacacc ggattgagat acagcgccgt cacgccaagc tttttcagat
    23521 acggcagttt ttcgcttatc ccgtccagat cgccgccata gaacgttgat ccgcccgcct
    23581 gcgccgtgac cggttcatcc cagtcacgca agatgatctc ttgtccggct gcatgatggt
    23641 aatagacatg atcctgttca gcttcacgag gaagactacg cgcaaaacga tcagggaaga
    23701 tctgataaaa aatctgatcc gcagcccatt gtgggccgat atccggtaca tcgacggcaa
    23761 actgctccag tcgtgccggc ggcattcggc tgaagccctg cggtgtaaac caacgctggc
    23821 gatcgtgcca cagcagtttg aaactgtaac gccgccgggg ttgtccgctg gagagatcaa
    23881 tcgccgcacg ccatgcggtg acgccaggct gctgctgact gcgatgctta tgcatcgata
    23941 ctgacatttc ttcgttatcg tgttctgtac gcagcataat gcgctgcggt gggtcttcgc
    24001 ccgtcagcca cagtgtaatg agcagttgat ctttgctttg tttaacaaat gggggcaccg
    24061 gcaggtgcca tgcatttaac atcataattc ccctgtgatg aaaatctgat caccttgcca
    24121 tagcctaatg caggatcact catcatctga aggatatttg ggggaggagg acgggggcgg
    24181 ataactttcg gaatagccgg atcaggattt cctcctgaac cggcttacag aacgcaagga
    24241 ttactggttt tcagccagct gacgatcgtg gcgacgctta aacatccagc caatcaacag
    24301 cacaacaatc cacgcgaaac cgacatacag cgagatacgc gtatccgggt gataaccaat
    24361 caacccgata ataaagagca ggaaaatcag cccgccgatg gtcgttgcta ccccacccgg
    24421 cactttaaat ttcagcgcct taacttcttc tggcggcaaa cagcgacgga aggcaatttg
    24481 cgacagcagg atcataatcc acacccacac cgtggcgaag gttgccagcg aagcgatcac
    24541 caggaagacg ttttccggca tgatgtagtt cagatacacc gcaaacagca gcgcggtagt
    24601 catcaccagc accgtaaccc acggaatacc gcgacgcgac gttttgctga aaattttcgg
    24661 cgcgctgccc tgctctgcca taccgtggag catacggcct acgccaaaga cgtcactgtt
    24721 aattgccgat agcgaagcgg tcagcacaac aaagttaaga atgctggcgg caaaggtaat
    24781 gcccatatgc tggaacgtca gcacgaacgg gctaccggca gtgccaacct gattccacgg
    24841 gtagatagac ataatgacga acagcgtacc gacgtagaac accagaatac gcatcggcac
    24901 ggagttaatc gcacgcggta tcgatttctc aggatctttc gcttcaccgg cggtaatccc
    24961 gataatttcg atcccaccgt aagcaaacat caccatttgc aacgacatca ccatcccaag
    25021 ccagccgtta ctgaagaagc cgccgttgct ccacagatta tgaataccgg tcggttgccc
    25081 gccgttgcca atcccccaga tgatgatgcc gaaaccagcg acaatcatga tgatgatggt
    25141 ggcgacttta aagaacgaga accagaattc cagctccccg aagaccttca cgctcatcag
    25201 gtttacggcg caaatgatca gcaccacgct cagtacccaa atccagtgcg gcaccgtcgg
    25261 gaaccagaca cccatataga taccaaaagc ggtcacatcg gcgatggcga caataaggat
    25321 ttcaaagcag taggtccagc cggtaatgta acctgccagc gggccgaggt tttcctgcgc
    25381 ataacgcgag aaagagctgg cggccgggtt atgtaccgac atttccccca gcgcacgcat
    25441 aatgatatac gccgcgatac caccgataat ataggccaac aacacgctcg gaccggccat
    25501 tttgatggcg tctgccgaac cgtaaaacag cccggtacca attgctgaac ccagtgccat
    25561 aaagcgtatg tggcgggtac ttagcccacg ctttagcttg ttcttacttt ccatcaattc
    25621 ctaacccatc aacacaataa aaaaccacgg ggcatcgcag ccccgtggtt aaaacaaatg
    25681 ttcagtgatt tagtgagcgc tggaggtcac ctgacgacct gccgcgcgat cccagataat
    25741 ggccagaacc accatcacca ctgttggcat taaccacgcc agaccttgtt cggccagcgg
    25801 taaacgctgc gcccaggacg gtaagatatc gctgaatgca gatgctttga tcccgtcgag
    25861 aataccaaaa agcaggctga taaacatcgg cggagcaatc acgcgggacg aattatgcca
    25921 ccatgagcgt gtaaaactta atacaaccag tgcgatacac ggcggataaa tagcggtcag
    25981 caccggtacg gagatctgga tcagctggct taagccgagg ttagaaacca ccatcgagaa
    26041 gccgccgagg ataaacacca gcgtacgata agagagcggt acgtactggg caaagaattc
    26101 tgcacaagca caggtcaggc caactgccgt taccaagcag gcgatgaaga ttaacgccgc
    26161 cagcaggaag ctaccgccgc cgccaaaggt gtgctgaacg taagcatgca gaatagcagc
    26221 gccgtttgca gactgatcga ccagcgacgc gctgtctgaa cccagacgga acagcgccag
    26281 gtacagcaga gtcagaccaa cacccgccat caggccagcc cagacggtat aacgggtcag
    26341 cagacgcgct tcggtaacgc cacgagaacg cgccgcgtta acaataacga taccaaacac
    26401 cattgcgccc agcgtatcca tggtcagata gccgttaacg aagccgttag aaaacgcagc
    26461 gttttgataa gcctcagtcg ccgtgctgat agaacccgcc ggccagacaa tcgcggcaac
    26521 agacaggatg accagcgcga taattttcag cggcgcaagg aagttgccca cggtatccag
    26581 cagcttgccc ggatagagcg aaaccagaat aacgatagcg aaatagacca ggctgtagat
    26641 aaacagcggc agcgcggaat caccagtcag cggcgcaatc cccacttcaa aggaaacggt
    26701 agctgtacgc ggcgtagcga aaagcggccc caccgccagg taacaaaccg ttgccagcag
    26761 cacgccagcg actttaccga ttggcgtgct gaggctgtca acaccgccgc caacttttgc
    26821 cagcgccact accgttaata ccggcaggcc aacggcagta atgaggaagc cgaatgccgc
    26881 agtccagacg tgttcgccag cctgcaagcc gaccattgga gggaaaataa tgttacctgc
    26941 gccgacgaac aacgcaaatg tcataaagcc cagagcgatg atatcgcgcg atcttaattg
    27001 atgggtcata aatcatactg cctgtggatg tggtgttgta acgttaaatt tttgtgccaa
    27061 tccctgggga tgatacagcg gtatatttaa acaattttag cgggaaatac tcccaaccag
    27121 gcgcggcgaa gaatagtttt tcgatttacc gcgttaatgc agtcagtatg acagcacctg
    27181 aagcgcaatt taaacgctta taacgtttta aagcaaggag ggatctaaaa accagaggta
    27241 tatagcgata agaaatcacc aaccagtata aaacactatt tccgtgaaaa agacatggct
    27301 aatcatgcga acaaaaaatc accaaccgtt gtaaaatcta acgcccgtat tttgactgca
    27361 aagaaaataa gccagcatcg actggcttat ggcaataaaa gatgacaaag gcggattaat
    27421 cgctgttttt ggcaattaaa cgttccggga taacaaaact gaaacgtgtt ccttttccta
    27481 ctgtactctc aatattcagg cgactttcgt gatgattcac agcatgtttc acgatcgcta
    27541 accctaatcc gctaccgccg gtttgccggg aacgcgcttt atcaacgcga taaaaacgct
    27601 cggtcaggcg cggaatatgc tccggtgcaa tgcccggtcc gttatcttca acgctaaatt
    27661 cggcaccgtg cggcactcgc tgccagcgta cggtgatatg cgtgccttct ggagtatgat
    27721 tcacggcgtt atagaccagg ttcgaaatcg cactgcgtag ctgatcttcg ttgccagaca
    27781 ctttgaggcc gttatctatc tcaaaggtaa atgtctgttt tttctgactc agagtctgag
    27841 cctcgcgctc aacaacgcgc agcatcatcg gcacatcaac cttttcattg agcaattgcg
    27901 tcggcgcggc ttcaattttc gacagcgtta gcaactgctt caccagccct tccatccgct
    27961 gggtctgctc gcgcatggtg tgcaacgctt tttcgcgtac cgcgccttcc agcggctgct
    28021 catccatcat ctccaggtaa ccctgtaaca cggtcaatgg cgtacgtaac tcatggctca
    28081 cgttggcaaa aaagttacgc cgcgcccctt ccagttgatg catttgcgtg acatcacgcg
    28141 ccaccatcag caactgtttg tgggtataag gcatgacgcg aatttccaga tgccgcccgg
    28201 tgttgagcac cagattgagc gggcgagaaa aatcacgcgt tttcagatat tgcgtaaact
    28261 ccgggtaacg cagtaggtta aggatgttct gcccgttatc ttccggccag cgcaaaccaa
    28321 gaatttgttg cgccagaccg ttacaccaga aaataccgct ctcttccgtg gtcagcacca
    28381 ccgcgtcggg cagcgactcc gcgccgctac gaaagcgttt aatcagattg cccagttcac
    28441 ggcggcgttt tttatttcgc agctgcatct ggtgtaagcc gtatagtagc ggttcccagc
    28501 taccacgccc cggtggcggg gtcatactgc gatccaccca cagccaccat gaaaggcgca
    28561 ataaattcca gaaatgccag ataagcagtc ctgttaccga tgccagcaaa aaccagggca
    28621 ggtaaccaaa aaatgcaccc aggatgaaag ccgggaggca gcaaagtagc agctccagca
    28681 ccagcctttt ccacgacagc cgttccagca cgtaagatac tcccgttaag aaatcataag
    28741 ccctgctctg cgtccgatga gcaaggcgtt aaaagcgggt tgaaaaacga tatcctgtac
    28801 cgcgcacggt ctgcaccatg cggtcatgcc cgccgggctc cagtgcttta cgcaggcgac
    28861 gaatgtggac atcgaccgtg cggtcttcca catacacgtt agttccccag acgtggttta
    28921 acagctgctc gcggctgtac acgcgctcag gatgcgtcat aaaaaagtgc agcagtttaa
    28981 attctgtcgg ccccatctcc agcggctctt cgcccgccat cactcggtga gatgtcgggt
    29041 cgagacttaa tccctgcatc tcaatcacct cttccaccgc cattggcgaa atacggcgca
    29101 ttaccgcttt gattcgcgcc accagctcct tcggcgaaaa cggcttggtg atatagtcat
    29161 ccgcgccggt ttcaaggccg cgcacgcgat cttcttcttc ccctctggcg gtcaacatca
    29221 ccactggaat atcccgggtc atcgactcgc gcttaaggtg tttgatgaac tggataccgg
    29281 agccgccagg taacatccag tcaaggagaa ttaaatccgg ccagggttca ttcagttgat
    29341 tcacagcact gtcataatct tccgcttcga ccggctgaaa gccattttgt tcgagcacga
    29401 agcagaccat ttcgcgaatt ggagcttcat cttctacgac cagaatacgt ctcgccatga
    29461 tttgccctgt tgtaataaat aggttgcgat cattaatgcg acgtcattat gcgtcagatt
    29521 tatgacagat ttatgaaaag ctcgtcgcac atatcttcag gttattgatt tccgtggcgc
    29581 agaaaaaagc aaatggcaca tctgtttggg tataatcgcg cccatgcttt ttcgccaggg
    29641 aaccgttatg cgcatccttc acacctcaga ctggcatctc ggccagaact tctacagtaa
    29701 aagccgcgaa gctgaacatc aggcttttct tgactggctg ctggagacag cacaaaccca
    29761 tcaggtggat gcgattattg ttgccggtga tgttttcgat accggctcgc cgcccagtta
    29821 cgcccgcacg ttatacaacc gttttgttgt caatttacag caaactggct gtcatctggt
    29881 ggtactggca ggaaaccatg actcggtcgc cacgctgaat gaatcgcgcg atatcatggc
    29941 gttcctcaat actaccgtgg tcgccagcgc cggacatgcg ccgcaaatct tgcctcgtcg
    30001 cgacgggacg ccaggcgcag tgctgtgccc cattccgttt ttacgtccgc gtgacattat
    30061 taccagccag gcggggctta acggtattga aaaacagcag catttactgg cagcgattac
    30121 cgattattac caacaacact atgccgatgc ctgcaaactg cgcggcgatc agcctctgcc
    30181 catcatcgcc acgggacatt taacgaccgt gggggccagt aaaagtgacg ccgtgcgtga
    30241 catttatatt ggcacgctgg acgcgtttcc ggcacaaaac tttccaccag ccgactacat
    30301 cgcgctcggg catattcacc gcgcacagat tattggcggc atggaacatg ttcgctattg
    30361 cggctccccc attccactga gttttgatga atgcggtaag agtaaatatg tccatctggt
    30421 gacattttca aacggcaaat tagagagcgt ggaaaacctg aacgtaccgg taacgcaacc
    30481 catggcagtg ctgaaaggcg atctggcgtc gattaccgca cagctggaac agtggcgcga
    30541 tgtatcgcag gagccacctg tctggctgga tatcgaaatc actactgatg agtatctgca
    30601 tgatattcag cgcaaaatcc aggcattaac cgaatcattg cctgtcgaag tattgctggt
    30661 acgtcggagt cgtgaacagc gcgagcgtgt gttagccagc caacagcgtg aaaccctcag
    30721 cgaactcagc gtcgaagagg tgttcaatcg ccgtctggca ctggaagaac tggatgaatc
    30781 gcagcagcaa cgtctgcagc atcttttcac cacgacgttg cataccctcg ccggagaaca
    30841 cgaagcatga aaattctcag cctgcgcctg aaaaacctga actcattaaa aggcgaatgg
    30901 aagattgatt tcacccgcga gccgttcgcc agcaacgggc tgtttgctat taccggccca
    30961 acaggtgcgg ggaaaaccac cctgct
//
LOCUS       DAZPDU010000034        30873 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZPDU010000034 DAZPDU010000000
VERSION     DAZPDU010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30873
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.034"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            473..988
                     /locus_tag="ACHURS_001405"
     CDS             473..988
                     /locus_tag="ACHURS_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR1237181.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            1206..1934
                     /gene="artP"
                     /locus_tag="ACHURS_001406"
     CDS             1206..1934
                     /gene="artP"
                     /locus_tag="ACHURS_001406"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFR1237182.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            1952..2683
                     /gene="artI"
                     /locus_tag="ACHURS_001407"
     CDS             1952..2683
                     /gene="artI"
                     /locus_tag="ACHURS_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFR1237183.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            2690..3406
                     /gene="artQ"
                     /locus_tag="ACHURS_001408"
     CDS             2690..3406
                     /gene="artQ"
                     /locus_tag="ACHURS_001408"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFR1237184.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            3406..4074
                     /gene="artM"
                     /locus_tag="ACHURS_001409"
     CDS             3406..4074
                     /gene="artM"
                     /locus_tag="ACHURS_001409"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFR1237185.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLALLLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            4366..5097
                     /gene="artJ"
                     /locus_tag="ACHURS_001410"
     CDS             4366..5097
                     /gene="artJ"
                     /locus_tag="ACHURS_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFR1237186.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDSVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(5272..6399)
                     /gene="rlmC"
                     /locus_tag="ACHURS_001411"
     CDS             complement(5272..6399)
                     /gene="rlmC"
                     /locus_tag="ACHURS_001411"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFR1237187.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGDVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVMTLLVKQ"
     gene            complement(6440..6928)
                     /locus_tag="ACHURS_001412"
     CDS             complement(6440..6928)
                     /locus_tag="ACHURS_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFR1237188.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(6988..7833)
                     /gene="potI"
                     /locus_tag="ACHURS_001413"
     CDS             complement(6988..7833)
                     /gene="potI"
                     /locus_tag="ACHURS_001413"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFR1237189.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            complement(7830..8783)
                     /gene="potH"
                     /locus_tag="ACHURS_001414"
     CDS             complement(7830..8783)
                     /gene="potH"
                     /locus_tag="ACHURS_001414"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFR1237190.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALLYIWLILLF
                     LLPFLIVFKISLGEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(8793..9926)
                     /gene="potG"
                     /locus_tag="ACHURS_001415"
     CDS             complement(8793..9926)
                     /gene="potG"
                     /locus_tag="ACHURS_001415"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFR1237191.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVHEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(10021..11133)
                     /gene="potF"
                     /locus_tag="ACHURS_001416"
     CDS             complement(10021..11133)
                     /gene="potF"
                     /locus_tag="ACHURS_001416"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFR1237192.1"
                     /translation="MTALNKKWLSGLAAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(11484..11960)
                     /locus_tag="ACHURS_001417"
     CDS             complement(11484..11960)
                     /locus_tag="ACHURS_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFR1237193.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(12048..12950)
                     /gene="rimK"
                     /locus_tag="ACHURS_001418"
     CDS             complement(12048..12950)
                     /gene="rimK"
                     /locus_tag="ACHURS_001418"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFR1237194.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(13011..13733)
                     /gene="nfsA"
                     /locus_tag="ACHURS_001419"
     CDS             complement(13011..13733)
                     /gene="nfsA"
                     /locus_tag="ACHURS_001419"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFR1237195.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(13717..14004)
                     /locus_tag="ACHURS_001420"
     CDS             complement(13717..14004)
                     /locus_tag="ACHURS_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFR1237196.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            14164..14421
                     /gene="grxA"
                     /locus_tag="ACHURS_001421"
     CDS             14164..14421
                     /gene="grxA"
                     /locus_tag="ACHURS_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFR1237197.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(14451..14828)
                     /locus_tag="ACHURS_001422"
     CDS             complement(14451..14828)
                     /locus_tag="ACHURS_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFR1237198.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            15098..16783
                     /locus_tag="ACHURS_001423"
     CDS             15098..16783
                     /locus_tag="ACHURS_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFR1237199.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(16958..17494)
                     /gene="rcdA"
                     /locus_tag="ACHURS_001424"
     CDS             complement(16958..17494)
                     /gene="rcdA"
                     /locus_tag="ACHURS_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFR1237200.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            17578..18786
                     /locus_tag="ACHURS_001425"
     CDS             17578..18786
                     /locus_tag="ACHURS_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1237201.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTRTAMENS"
     gene            18786..19601
                     /gene="ybjI"
                     /locus_tag="ACHURS_001426"
     CDS             18786..19601
                     /gene="ybjI"
                     /locus_tag="ACHURS_001426"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFR1237202.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLLSFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            19687..19971
                     /gene="ybjH"
                     /locus_tag="ACHURS_001427"
     CDS             19687..19971
                     /gene="ybjH"
                     /locus_tag="ACHURS_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFR1237203.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTGDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYARHLASVGEGMVIEHQIDDHHH"
     gene            complement(20012..21239)
                     /gene="mdfA"
                     /locus_tag="ACHURS_001428"
                     /pseudo
     CDS             complement(20012..21239)
                     /gene="mdfA"
                     /locus_tag="ACHURS_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
     gene            21524..22120
                     /gene="ybjG"
                     /locus_tag="ACHURS_001429"
     CDS             21524..22120
                     /gene="ybjG"
                     /locus_tag="ACHURS_001429"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFR1237204.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            22178..22936
                     /gene="deoR"
                     /locus_tag="ACHURS_001430"
     CDS             22178..22936
                     /gene="deoR"
                     /locus_tag="ACHURS_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFR1237205.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFNPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(22983..24185)
                     /gene="dacC"
                     /locus_tag="ACHURS_001431"
     CDS             complement(22983..24185)
                     /gene="dacC"
                     /locus_tag="ACHURS_001431"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFR1237206.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            24432..25057
                     /gene="gstB"
                     /locus_tag="ACHURS_001432"
                     /pseudo
     CDS             24432..25057
                     /gene="gstB"
                     /locus_tag="ACHURS_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
     gene            complement(25054..26169)
                     /gene="yliI"
                     /locus_tag="ACHURS_001433"
     CDS             complement(25054..26169)
                     /gene="yliI"
                     /locus_tag="ACHURS_001433"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFR1237207.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(26280..26663)
                     /gene="bssR"
                     /locus_tag="ACHURS_001434"
     CDS             complement(26280..26663)
                     /gene="bssR"
                     /locus_tag="ACHURS_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFR1237208.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            26876..28201
                     /gene="rimO"
                     /locus_tag="ACHURS_001435"
     CDS             26876..28201
                     /gene="rimO"
                     /locus_tag="ACHURS_001435"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFR1237209.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPSETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(28248..29569)
                     /locus_tag="ACHURS_001436"
                     /pseudo
     CDS             complement(28248..29569)
                     /locus_tag="ACHURS_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(29577..>30873)
                     /locus_tag="ACHURS_001437"
     CDS             complement(29577..>30873)
                     /locus_tag="ACHURS_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
                     /protein_id="HFR1237210.1"
                     /translation="SIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQYLA
                     VGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCRENV
                     QTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDHMFS
                     EFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCRWVS
                     AERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINVSPW
                     QLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRISID
                     DFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAEGVE
                     SEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
BASE COUNT         7781 a         7963 c         7480 g         7649 t
ORIGIN      
        1 ttatcgagag tcaaagctgg gactatcaaa tttcgctcat tttcaaaaat acaacgtaat
       61 tttggcaatc atcttaatcg caggatctct tatcgcatgt gaaaaatttt gtaatgatta
      121 gacaataacc gcgttcattt atcacgcgtt ataaatgtga aactggcttc ttgcagatgc
      181 tcttaacctc tacaggagga tttataccac catttcagaa ctatcatatt taccttaaat
      241 gatcatttca gccatataac acgctcaacc tcacaaatgg tttacactgt ctattgtttg
      301 ggtaatttaa gtaagtcagc tacgagcaac cttgagtgga tagccagcaa tggactcaaa
      361 cccaatcagg tgttcagatt gtttcagaat tgtattaaaa acaatgtatt aaataatctg
      421 atagcgtttg gtcgtagtgt gacgcagaca gcaagctaat aaggaaatga ttatgcgcta
      481 ccgttcacta tctctactta ttccatgtgc actgctgctg agtgcctgta ccaccgtcac
      541 gccagcttat aaagataacg gtccccgcac cggttcctgt gtccaggggg ggcctgacag
      601 cgtggctcaa caattctatg actaccgcat tcaacatcgc agcaatgaca ttaccgccct
      661 gcgcccctat ttgagtgaca aactggcaac ccttcttagc gatgccagcc gcgataatag
      721 tcatcgtgaa ttgttaagca gcgatccgtt ctccagtcga accaccttgc cagatagcgc
      781 ccacgttgcc agtgcatcga ctatccctaa tcgtgatgcc cgtaacattc cattgcgcgt
      841 agatctcaaa cagggtgacc agggttggca ggatgaagtg ttaatgattc aggaaggcca
      901 gtgttgggtc atcgacgatg tgcgctactt aggcggcagc gttcatgcca cggcgggtac
      961 gctccgtcag tccatcgaga atcgctaacg ccttgatcaa ccaataaacc ccgtaaattg
     1021 tccggcattt cgtgaggaat gccgacattt atgctcgccg accaccgccc ccgttatttt
     1081 gtgctatgtt tattgaataa tgcgctttgc ttttaacttt taaagcagaa atattgcata
     1141 attattctgt caaaggtact atcttcgacc tcaatagcta tcagactgcc agtatacgag
     1201 tgtcaatgag tattcaatta aacggcatta attgcttcta cggcgcgcat caggcgctgt
     1261 tcgatatcac gctggattgc ccacagggcg aaacgctggt gttacttggc cccagcggcg
     1321 cgggtaaaag ctcgctgctg cgtgtactca atctgcttga gatgccacgc tccggtacgc
     1381 tcaacattgc aggcaaccat ttcgatttca ccaaaacacc ctctgacaaa gcgattcgcg
     1441 atttgcgtcg taacgttggc atggtgtttc agcaatacaa cctgtggccg catctgaccg
     1501 tgcagcaaaa cctgattgaa gcgccctgcc gtgtactggg gttgagtaaa gatcaggcgc
     1561 tggcccgtgc agaaaaactg ctggaacgtc tgcgtctcaa accttatagc gatcgttacc
     1621 cgctgcatct ttctggtggt cagcagcagc gtgttgctat tgcccgtgcg ttgatgatgg
     1681 aaccgcaggt actgctgttc gatgaaccga ccgccgcact ggacccggaa attacggcac
     1741 aaatcgtcag catcattcgt gagctggcag aaacgaatat tacccaggtg atcgtcaccc
     1801 acgaagttga agtggcgcgt aaaaccgcca gccgagtggt gtatatggaa aatggtcata
     1861 tcgtagaaca aggcgacgcg agctgcttta ccgagccgca aaccgaagca tttaaaaact
     1921 atctctctca ctaagattcg ggataacaac aatgaaaaaa gttctgattg ccgcgttaat
     1981 tgcaggtttt agtctttccg ccacagctgc cgaaaccatt cgttttgcta ccgaagcatc
     2041 ctatcctccg tttgaatcga ttgatgcaaa caaccagatc gttggttttg acgtcgacct
     2101 ggcacaagcg ctgtgtaaag agattgatgc aacctgcact ttctctaacc aggcgtttga
     2161 cagcctgatc ccaagcctga aattccgtcg cgtagaagcc gtgatggcgg gcatggatat
     2221 cactccggag cgtgaaaagc aggtgctgtt taccaccccg tactatgaca actctgccct
     2281 gtttgtgggt cagcaaggca aatacaccag tgttgatcag ctgaaaggca aaaaagtcgg
     2341 cgtacagaac gggacgacac accagaaatt cattatggat aagcacccgg aaatcactac
     2401 cgttccgtat gacagctacc agaacgcaaa actggatctg caaaacgggc gtatcgacgg
     2461 cgtcttcggt gacaccgcag tggtcactga gtggctgaaa gataacccga aactggcggc
     2521 ggtgggcgac aaagtgaccg ataaagatta cttcggcact ggcctcggca tcgcggtacg
     2581 tcagggcaac actgagctgc agcagaaact caacactgcg ctggaaaaag tgaagaaaga
     2641 tggcacttac gaaaccatct acaacaaatg gttccagaag taattcctga tgaatgaatt
     2701 ttttccttta gcaagcgccg ccgggatgac cgtcggcctt gccgtttgtg cattgattgt
     2761 cgggctggcg ctggcgatgt tctttgcggt atgggagtcg gcaaaatggc gtcctgtcgc
     2821 gtgggcaggt tcagcgctgg taaccattct gcgtggcctg ccagaaattc tggtggtgct
     2881 gtttatctat tttggctcct cgcagctgct gctgacgctt tcggatggct tcactatcaa
     2941 tcttgggttc gtgcagatcc cagtgcagat ggacattgag aacttcgacg ttagcccgtt
     3001 cctttgtggt gtcatcgctc tgtcactgct gtatgccgcc tatgcctcgc aaacgcttcg
     3061 gggcgcgttg aaagcggtgc cggtgggtca gtgggagtct ggtcaggcgc tggggctgtc
     3121 gaaatcggct atctttttcc gtctggtgat gccgcagatg tggcgtcatg cgctgcctgg
     3181 cctcggtaac cagtggctgg tgctgctgaa agataccgcg ctggtcagtt tgattagtgt
     3241 gaatgattta atgctgcaaa caaaaagcat cgctactcgt acccaggaac catttacctg
     3301 gtacattgtg gcggcggcga tttacctggt gatcaccctg ctcagtcagt acattctcaa
     3361 acgcattgac ctgcgcgcga cacgttttga gcggaggccc agctaatgtt tgagtattta
     3421 cccgaactga tgaaagggct acacaccagc ctgacgctaa ccgttgcctc gctgattgtg
     3481 gcactgattc tggcattgat ttttaccatc atcctgacgc tgaaaacgcc ggtgctggtg
     3541 tggctggtgc ggggttatat cacgctgttt accggtacgc cgctgctggt gcagatcttc
     3601 ctgatttatt acgggccggg ccagtttccg acgttgcagg agtatccggc actgtggcat
     3661 ttgttgtcag aaccgtggtt atgtgcgctg attgcgttat cgctgaacag tgcggcgtat
     3721 accacgcagc tgttttacgg tgcaattcgt gcgatcccag aaggtcagtg gcagtcctgt
     3781 agcgccctgg gaatgagcaa aaaagatacg ctggcgctcc tgctgccgta tgcctttaaa
     3841 cgctcgctct cttcttattc caacgaagtg gtgctggtgt tcaaaagtac ctctctggct
     3901 tacaccatta cgctgatgga agtgatggga tacagccagt tgttgtacgg acgcacctac
     3961 gatgtaatgg tgttcggtgc ggcagggatt atttacctgg tcgtcaacgg cctgctgacg
     4021 ctgatgatgc gtctgatcga gcgcaaagca ctggcattcg aacggcggaa ttaatgtagc
     4081 tgcgtacatt cgccgggggg gtttgtaggt ctgataagac gcgccagcgt cgcatcagac
     4141 atcagcacgg tgcctgtgcc ggatgcggcg tggacgcctt atccggccta catagtgcat
     4201 aaattcttat aatgaagacg gacaactcac taagttatcc gtcttttttt atttcattta
     4261 aattatttaa tcatatttat tgcatataaa ttcacttgat ggcattgtta tcccatgccg
     4321 cagacacggc caaaaatcat aagattgaca gacgggagtt ccatcatgaa aaagttagtt
     4381 cttgccgctt tacttgcttc ctttactttc ggtgcttctg ccgcagagaa aatcaatttt
     4441 ggcgtttcag ccacctatcc accctttgaa tctataggtg ctaataatga gattgtcggc
     4501 tttgatatcg atctggcaaa agccttgtgc aaacaaatgc aggcagaatg tacttttact
     4561 aatcacgcgt tcgacagcct gatcccgtca ctgaaattca gaaaatatga ctccgtaatc
     4621 tccggtatgg atatcacgcc cgagcgtagc aagcaggtat cgtttaccac gccctactat
     4681 gaaaactcag ccgtcgtgat tgccaaaaaa gatacctaca aaacgtttgc cgatctgaaa
     4741 ggcaaacgta ttgggatgga aaacggcacc acgcaccaga aatatattca ggatcagcac
     4801 cccgaagtga aaactgtctc ctatgacagt tatcagaatg cctttatcga tctgaaaaat
     4861 ggtcgtattg atggggtatt tggtgacaca gcggtggtaa acgaatggct gaaaaccaat
     4921 ccgcaactgg gcgttgctac tgaaaaagtg accgatccgc aatacttcgg caccggtctg
     4981 ggcatcgctg tacgtccgga taacaaagct ctgctggaaa aactgaataa cgcgctggca
     5041 gcaattaaag ctgacggtac atatcagaaa atcaatgacc agtggttccc gcagtaattg
     5101 agactgatga caaaagtaag attgcctgat gcgctgcgct tatctggccc acatcaactc
     5161 tgcaatgtat tgatttagca tgattttgta ggccggataa ggcgtacacg ccgcatccgg
     5221 catgaacaaa gcgcacctga tcagcaatct gaagccgcac ctgcggcttt tttattgctt
     5281 caccagcagc gtcatcactt catagtgcgc ggtgtgcggg aacatatcaa aaagctgtac
     5341 ccgttcgatg cggtaacctg gcagttcgcg gatatctttc gccatggttt gggcgttaca
     5401 gctggagtag ataataaaac gcggtgccat cgttgagaga taatcacaca gcggtttacc
     5461 aatgccgcgg cgcggcgggt taaccagcac cagctccggc acatccccct gagcggtggc
     5521 aaactgagtg gagtccagcg cctgaaattg caaacgcgtt aagcccagtt cagcggctga
     5581 ctgctttgca caggcaatgg cctctgatgc aatttcgatc ccggttaact gcatgtcagg
     5641 cgtcgcgcag tgtaaaccaa agccacccac accgcagaac agatcccaca tatgtttaac
     5701 tggcagctgc cgtacccagt cgcgcgcggt agcgtacagc tggctggcga ccgccggatt
     5761 agtctggaag aaactttgcg gacggatcca cagcggtacg tcattaaaac gctctgccag
     5821 cgcctgttgt tcagtcaggt agatctccgt ctccccttcc ataatcgcca tatgtaccgg
     5881 ctgaatattg acggtaataa ctttcagttg cggtagttgt tcctgtaacc acggcagcgc
     5941 cttacgcagt tgtgccagtt tggtttcaga acgcagtaca aagcgcagca tcatgccgcc
     6001 atcgctctgg ctttcagtca gcagaatgta tttcagttcg ccacgcttac gcgccacgtt
     6061 gtagggcgtt aaccccgcac gggcgataaa cggttttagc gccgcaaaaa cgggcgcaaa
     6121 agaggcagga taaagcgggc agtcacaaag gtcttccggc gtgccatcgc gatgcagcat
     6181 accgagcagt ggtttttcaa cgctaccact caccaccatt ttggctttat tacgaaaccc
     6241 ttgttccggg cctgacaccg gcgcgcacca ttcctcaacc ggaaaatcgg cgagcagatt
     6301 tttaagatcg gcggttttag cggagagttg ctctggaatc ggctgcgtta tccactgaca
     6361 ggaacgacag cgacccgcgt cgtaaagtgc gcactgcata cattgacctt cacatcatca
     6421 ggggcgacga ttatacacat tattgcaact ggaagaaccg ccgactggtc gagggaacga
     6481 acagcagcat caggatgagc atatccggca gcttctgcag catcaggcta tggaagattt
     6541 cacgttttga ttcaccggga atgctgaaca gctccggata accgtacccc agcgaagccg
     6601 cccacaagta acttgcggcg gtgatttgtg tcagcagata tagccagcgc gcccagcgac
     6661 gccctttcac cagtgaaaac gcacaccaga tctcaatgaa aaccagcacc agactgctta
     6721 aaaagaccag cgttaaactc caggtttgta cgctgcgatg aatgaactcg ccaataccgc
     6781 gcacgcccag cgtattgaaa atcatcagca cgtcgaggcc acggatcata ataatggcga
     6841 gcgccgccac ctgcaccagc gcaggcacat tcaggcgagc atgagatgaa gatgttttct
     6901 taaaaaatcc caacgtttcg tcttccatga aaacaatgcc gcggcatgcg cggcattatg
     6961 tagccaggtt ggcaaatttt agtgtcttca gccatgtctt gcacgctgga tatcgcgtat
     7021 ccgctgtttt tctgcgcgag ccatcagata ccaggcgata aatccgacaa ttccgaccgc
     7081 accgagaatt aacgttgcaa gggcgttgat ttccggattc actcccatcc gcacgctgga
     7141 aaagaccagc atcggtaacg tggtggctcc cggcccggaa acaaagctgg cgatcaccag
     7201 atcatcaagc gacaaagtaa aagccagtaa ccagccagaa atgatcgcgg gcatgatcat
     7261 cggtagcgta atgacgaaaa acactttcag cggcgtcgca ccgagatcca tcgctgcttc
     7321 ttctatcgag cgatccagtt cccgcaatct tgaagaaata acgaccgcca cataagccgt
     7381 acaaaaagtg acatgcgcca gccagatggt gagcataccg cggtccgcag gccagccaat
     7441 ggcatgagca agcgcgacga ataataacaa cagcgacaag cccgtgatga catctggcat
     7501 caccagcggc gcggtgatca taaaggcaaa accatttgat ccgcgaaacc tgccaaaacg
     7561 caccagcacc accgccgcaa tagtcccgag gatcgccgcc gccgttgccg cacaggcagc
     7621 aattgtcagg cttaaaccaa ccgcactcat catcgcgtca tcgcgcaata actcaccata
     7681 ccagcgcgtt gaccagccgg cccacaccgt caccagcttc gagctgttaa acgaatagat
     7741 aaccagcatc agcattggcg cgtagagaaa ggtaaagccc agcagcaaaa tcacaatccg
     7801 ccagggcgaa cgaactaccg gtaaattatt catccgtgtt ctcccacgct tttttgctgg
     7861 tgtttgtgga accacattat cggcacaatt agcagcagca acatgatgat cgctaccgcc
     7921 gaggccaccg gccagtcgcg gttattgaaa aactcttgcc ataacacgcg cccgatcatg
     7981 atgctgtccg ggccaccgag cagttccggg atcacaaact cgcccacagc cgggataaac
     8041 accagcatcg atccggcaat aatcccacct ttagtcagcg gcacgatcac agtaaagaac
     8101 gttttcagcg gtcgtgcacc gagatccagc gctgcttcca ccagcgaata atcaatacga
     8161 atcaacgcgg tataaatcgg cagtaccata aacggcacgt aagcgtaaac aatgccgata
     8221 taaacggcca gattggtatg cagaatggtc agcggttgat cgataacccc cagccacagc
     8281 agaaaattat tcagcacacc gttgtttttt aatattccca tccaggcata aacgcggatc
     8341 agaaacgagg tccacgacgg caggatcacc agtagtaata aaatattacg ggtcgaaggc
     8401 ttactgtgcg ccaccgccca cgccagcgga tagccgatca gtaaacagca aaatgtcgaa
     8461 atcgccgcca cctgtaacga ctggagataa gcatcaaaat agagcggatc gtcggtcagt
     8521 tgcagaaaat taccgagatt aagagtgatg gaaagttgcc cgtcagccca ctccatcagt
     8581 tcggtataag gtggaatagc gcgcgccatc tctcccaggc ttattttaaa gacaatcaga
     8641 aatggcagca gaaacagcaa gatcaaccag atatatagca acgcaatgac cagtttgcgc
     8701 ccatgcttca tttgcagctg cgacaaccac agcttaaatc cgcccggcgg tttcgactgg
     8761 gcagcaggtt caagtgtact catctttgct ccttaaaccg tcagcaccac acagctgtcc
     8821 acttcccagc acaaacgcac ttcgtcgccc caggtcggta accctttacg atgacgatgg
     8881 gcgttttgta gctgggcgct gatcatctgc ccacttttca gtcgaacgtg atacaccgaa
     8941 agatcgccga gataggcaat gtgtatcacc tcccccaccg cgaagttaca accattggcg
     9001 ggcggctctt cgcaaagcat gattttttcc gggcgcagcg ctacgtgtac cggcacgtta
     9061 tcgaccactg aagcatccgc atcgactttc agtggatgca ccagccccgg cgaatcaagc
     9121 accaggccat cttcctgacg ctctttgagt acaccttcaa agacattaac cgaaccgata
     9181 aattcagcgc tgtagcgggt agttggatgc tcgtagatct cttccggctc gccaatctgg
     9241 acaaatttcc cgcgattcat aatggcgata cgccccgcca tggtcatcgc ctcttcctga
     9301 tcgtgggtga ccatcacaca agtcacaccg acgcgctcca gaatatccac cacttcaagc
     9361 tgcatccggt cacgcagctt tttatccagc gcgcccatcg gctcatcgag cagtaatagt
     9421 ttcgggcgtt tggcaaggct tcgggccagg gccacacgct gtcgctgacc accggaaagc
     9481 tgatgcggtt tgcgtttggc gaactcctgc atgtgcacca ggccgagcat ctcatggacc
     9541 cgactggcaa tttccgcttt cggtagtttg tcctgtttca ggccaaaagc gatgttctgt
     9601 tccacggtca tatgcggaaa cagcgcgtaa gactgaaaca tcatattgat ggggcgcagg
     9661 taaggcggca cctgtgacaa atcgacgcca tcgagcatta tctgcccggc agaaggttgt
     9721 tcaaaacctg ccagcatacg cagcagcgtg gacttgccac agccggatgc gcccagcagc
     9781 gcgaagattt cacctttgta gatggtcagg ctgacatcat cgaccgcatg ctgaccatcg
     9841 taggatttgg tgaggttgcg gatttctaat agcggcgtca gcgccttacg ggttttcgcc
     9901 tgcgggcgag ggatagcgtc attcacggct gctctccggc attgaatgtg tgcaagcgca
     9961 ccatgatggt gcgaattgcc ggctggtgtg gtgcgcccgc ccggcatcta aggctgcgga
    10021 ttattttccg ctcttcactt tggtccacgc gcgggtgcgc acacggtcga ttttcggatc
    10081 ctgtactttc agagtgaata gcttcgcacg aacgtccgcc ggaggataaa tacccgggtt
    10141 ctcacggact tccgcactca ccagcggcgt ggctgcttta ttggcgttgg catagaacac
    10201 atggtcggaa atatgcgcta ctacatccgg gcgcagcagg taattcagga actgataggc
    10261 ttcgtctttg tttttggcat ccgcaggcat ggcgaataca tcaaagaacg ccatcgcccc
    10321 ttcttttgga atcgagaacg agacattcac tccattcttc gcttccttcg cgcggtttga
    10381 cgcctgccag acatcacctg cccagccgat agcgacgcaa atatcgccgt ttgccaggtc
    10441 gttaatgtat tgagatgaat ggaaataacg aatgttcggg cgcagcttta acagcagatc
    10501 ggttgccggt ccggtgtaat catccgcttt agtgctgttg ggatctttgc cgagataatt
    10561 caacacggta gcaaaaactt cttctggcgc atccaggaaa gagacaccgc agcttttcag
    10621 tttttccaga ttttcaggtt tgaggatcaa gtcccagcta tcgacgggcg cgttttcgcc
    10681 cagcacagct ttaactttat caacgttata gccaatcccg gtcgtcgccc acatataggg
    10741 catagcaaat ttattgtcgg gatcgtgttt ggcgaccagc ttcagcagct ccgggtcgag
    10801 attcttccac tccggcaatt tgcttttgtc cagcggctgg aaaactcccg cagtcaactg
    10861 gcgttcaaga aagctggcag atggaaccac caagtcaaag ccggtgctac ccgccattaa
    10921 tttgccttcc agtacttcgt tagagtcgaa aacatcgtag acgactttaa taccggtttc
    10981 tttttcaaaa ttggccaccg tgtccggggc gatataatca gaccagttat aaatgtggag
    11041 tgttttttgt tcagccgcga gcgtgccgac agagacggcc atcagagcac ccgcagccag
    11101 acccgaaagc cattttttat ttaaggcggt catatctctt tccttctgaa agttcgttaa
    11161 caaacgaaca aaagttgtca cactaaaaga tatgcaataa acgtgcatat tttttatcgg
    11221 cggggtgtaa gaaaagagaa ctctctcccg gccagcactg ctcgctatat aaaagcatcg
    11281 caagaataac tttagccagg gtttgagact atagctcgga ttaaaaaacg gtatttaaca
    11341 aatttttatg cgtatttgct ctatgtgata aagccaaatc gacacaagag aagtgaaata
    11401 atctttaaga aaaggttaaa aacagcagat aattttgcag cgcgcagcca ctgcaccagc
    11461 gactgcgtgc tcgaaaggag ttatcagtgg aggaaatagt tttcggtaac gttgttttgc
    11521 ccttcatcgt cagtcggtaa cagcagttga tgggcattag cttcaagaat gaccatcgaa
    11581 atctgctctt cgctctgacg cacgaaccat gcaaactgct cataggttac gccctgcata
    11641 acggataaag actggcaaac caccagcttt ggcagattat catcctgcat atcaagaaat
    11701 gctttcacgg tcagcgaact ggcattgatg gcagataaat ccgccgccag cggcaatacg
    11761 gctgaaggtc ggacttccgc catggcagaa aacaggatcg tgttatcgat cagatcgatt
    11821 ttggcatcaa agacaccgtc gaaattctgc atatggggca gatgcagagc ctgacagtta
    11881 tcacattcaa aaaaactcat ccccaggtca tcaagccatt gacgcagcgt atccagacca
    11941 ggaacgacca gcgatgtcat aattttgtac aacctctttc gataaaaaga ccggcacagc
    12001 ttacgcaaaa agcgcaggca aaaccatgat cagtaatgtg attgcgacta accacccgtt
    12061 ttcaggcaat attctgtcgt agcgtggcgt tcgatccagc ggatcatttt acccgcgatg
    12121 tcgataccag tggttttttc tattccttcc agccccggcg acgcattcac ttccatcacc
    12181 aacggcccgc gattagcacg cagaatatcc acaccagcaa cgtccagcgc catcgttcgc
    12241 gcggctttta tcgcgatttc acgctcctgt ggtgtgatac tggcgacgct tgccgcgccg
    12301 ccacgatgca aattggaacg aaaatcgccc tctttcgccc gccgttcaat cgcagcgacc
    12361 acttcatcgc caacaaccag acagcggata tcgcaccctt gcgcctcttt gatatattcc
    12421 tgcaccagaa tatgcgcgtt cagaccgcgg aaagcgtcaa tcacgctttc cgccgcctga
    12481 cgcgtctccg ccagcacgac gccaattccc tgcgtgcctt caaccaactt gaccaccagc
    12541 ggcgcaccac cgaccatgtc gattaaatcg ctggtatcat ccggcgaatg cgcaatgccc
    12601 gtgacaggca ggtcgatgcc ctgacgcgcc agcagttgca tggaacgcaa tttgtcacgc
    12661 gcccgggcaa tggcgaccga ctcattgagc ggatagctcc ccagcatctc gaactggcgc
    12721 agtgccgccg tcccataaaa ggtaatggcg gtgccaatac gcgggatcac tgcgtcaaaa
    12781 tggggtaact tgcggccttt gtagtgaata gaagacgccg caggatttat gttcatgtag
    12841 caagaaagcg gatcaagaat ttcaaccagg tgaccgcgct gtatagcggc ttcacgcagc
    12901 cgcttacacg aatagagcgt tccatcccgg gacaatatgg caattttcac cctgcacctc
    12961 tctgtcagac ctggtcaaaa gcccgcgtat catacatcga cgtggcggtt ttagcgcgtc
    13021 gcccaaccct gtttgtgcaa ataatccaga ataaatgggc ggctttcttt aatgattgtt
    13081 cggcggatat gatcgctcca ggtatcccgg cgattattgc tgccacgggt gaggtaatat
    13141 tccgccagtt gctcgtcata ctgcgccagt gcgtctttat ccagcggttg atagctgttt
    13201 tcatgcacca aaatggaggc cggtaaacgc ggcttgagat ccggattatc cgcaggccag
    13261 ccaaggcaca gcccaaacag cggcagaaca tgctgcggta atttcagcag tttcgtcacc
    13321 gcttcaatat tattgcgcag gccgccgata tataccccgc ccaatcccag cgattccgct
    13381 gcggttaatg cattctgcgc catcattgcc gtatcaacga caccgagcaa cagttgttcc
    13441 gccaggccga gctgagcatc cggacagatc tgtaaatggc ggttaaagtc ggcacagaac
    13501 acccagaact ccgccgcttg cgctacgtgt ttttgcccgc cggtcagcgt caccagttct
    13561 tcacgtaatg ctttgtcggt aatgcgaata atgctactac actgcaaaaa actggaactg
    13621 gacgtcgcac gggcgctgtt aataatcgcc tcacactgcg cttcggaaat gggttcatca
    13681 gtgaaatggc gaatggagcg atggccacaa ataagttcaa tggttggcgt cattatcttt
    13741 ttctctttct gaacgtgaat attgcggtgg acggttcatc agctgtgggg caagacgttt
    13801 tgccacctga agaataacca ccaccgcagc gggaagcatg agcaaaacac cgagaaaaat
    13861 catcagaatc tgcacttctg gccgagaaaa tggctcaggc agcgacaggg agtcgcttac
    13921 cgacagcagc gccaccgcca gtagcatcat tccgataaat tccagtatca acacgccttt
    13981 aggcaattta ccgatcgcgc gcatacgctt ccctctgcaa agtgagcctt cagtctaaaa
    14041 cttttcactg tattgtgttt aacagttata gcttttagca attaatgcaa caggttaaac
    14101 ctactttcag cgaatacatt ttagcgtgat cattacaggc ataaatctat gaggagagaa
    14161 ataatgcaaa ccgttatttt tggtcgttcg ggttgccctt actgtgtgcg tgcaaaagat
    14221 ctggctgaga aattgagcaa tgaacgcgat gattttcagt atcagtatgt agatattcgt
    14281 gcggaaggga tcactaaaga agatctacaa caaaaggcag gtaaacccgt agaaaccgtg
    14341 ccgcagattt ttgtcgatca gcaacatatc ggcggctata ccgattttgc tgcatgggtg
    14401 aaagaaaatc tggacgcctg atcgtctgac aagccctcgc gttgagggct ttactgattt
    14461 tttctgtgct gtggtttaaa caaactactg ataaataaga aacacagtgc ccccagcgca
    14521 caccagaaca ccgcgcttag taaccatgcc agctcttgcc agaatgagcg cgtcggtgaa
    14581 aaaaacagcc gcataatgag cattgaacag ggtgccgcca gcattgcgcc aaatagaggt
    14641 ttcaggactt ctctacgctg tgaaaagaaa ctggcaactg ctccaggaag aatgaaaaac
    14701 agcaagccga tttcaggatg cccggcagcc cgaaaagcgc ctttcatgtg cgtcgccaga
    14761 aaaaggcaca ccacaatgaa gaggacaaaa cagcagattg cccccgccca acgttgttta
    14821 tgtttcactc gttcctcctg acactgcgtc tatcgaacac atttttcgcc agtgtggcgt
    14881 tcagtaagat aaagccgctt cgcattccat gctaatatag gccaacgcaa ttcatatagc
    14941 cgttgatacc taatgtgatt acactagtaa aatatattgt tactttacta tcgtttaggt
    15001 gcgctgaatg aatctgcgcc ctgaattctg gtaaaaaaca ttatcgtaaa ttaccatttc
    15061 tttcaacagc ttactagtaa acaagaagtt agcctccgtg aatataaacg tcgccgaatt
    15121 gttaaatggg aattacattc tgttattatt tgtggtcctc gcgcttgggc tatgtctcgg
    15181 aaagttacga cttggttcga tccaactggg taattccatt ggcgttttag tcgtatcgct
    15241 gttattaggc caacaacatt tcagcattaa caccgatgcg cttaatcttg gctttatgct
    15301 gtttattttc tgcgtcgggg tcgaagccgg accgaacttt ttttccattt tttttcgcga
    15361 tgggaaaaat tacctaatgt tagcactggt gatggttggc agtgcgctgg tgatcgcctt
    15421 agggttaggt aagctgtttg gctgggatat tggcctgacg gccggtatgt tagcaggctc
    15481 tatgacgtcg acaccggttc tggtcggtgc tggcgataca ctgcgtcatt ccggcatgga
    15541 aagcaggcag ctctcactgg cactggataa tctgagcctc gggtatgcct taacctattt
    15601 aatcggtctg gtgagtttga ttgttggtgc gcgttacttg ccgaaattgc agcatcagga
    15661 cttacagacc agcgcccagc aaatcgcccg cgaacgtggc ctggacactg atgccaaccg
    15721 taaggtttat ttaccggtga tccgcgccta tcgcgtcggc ccggagctgg tggcctggac
    15781 cgacggcaaa aatctgcgtg aactgggtat ttatcgacaa accggctgct acattgaacg
    15841 tattcgacgt aacgggattc tggcaaatcc agacggtgat gccgtgctac aaatgggcga
    15901 tgaaatagcg ttggtaggct atcccgacgc ccatgcccga ctcgatccca gcttccgtaa
    15961 cggtaaagaa gttttcgatc gtgaccttct cgacatgcgt atcgtcactg aagaagtggt
    16021 cgttaaaaac cataacgctg taggtaaacg tctcgcacaa ctgaagttga ccgatcacgg
    16081 ttgcttcctt aaccgcgtca ttcgtagcca gattgagatg ccgatagatg acaacgtcgt
    16141 gcttaacaaa ggtgacgttt tacaagtcag cggtgatgcc cgtcgcgtaa aaaccatcgc
    16201 cgatcgcatc ggctttatct cgattcacag ccaggtcact gacctgctgg cattttgcgc
    16261 cttctttgtt attgggctga tgatcgggat gatcaccttc cagttcagca cattcagttt
    16321 cggcatgggg aacgctgccg ggttgttatt cgccggaatt atgctgggct ttatgcgtgc
    16381 taaccacccg accttcggtt acattccgca aggtgcatta agcatggtga aagagttcgg
    16441 cttgatggtg tttatggcag gcgttggtct gagcgccggt agcggtatta ataacggcct
    16501 gggcgcgatt ggcggtcaga tgttgattgc cggattaatt gtcagtcttg tgcccgtggt
    16561 tatctgtttc ttgttcggtg cttatgtatt gcgaatgaac cgcgcactgt tgttcggcgc
    16621 aatgatgggc gcacgcacct gcgcgccggc aatggagatc atcagtgata cagctcgcag
    16681 taacatccct gcgctgggct atgcgggcac ctacgcaatc gccaacgtcc tgctgacgct
    16741 ggcagggaca atcatcgtca tggtatggcc aggattagga taaaactgaa gttgccctga
    16801 aaatgaaatt tttttgcaca accgcagaac ttttccgcag ggcatcagtc ttaattagtg
    16861 ccactgcttt tctttgatgt ccccattttg tggagcccat caaccccgcc atttcggttc
    16921 aaggttgatg ggttttttgt tatctaaaat ttatctacta ccctgcaacc ctctcaacca
    16981 tcctcaaaat ctcctcgcgc gatagcggct tacggtcggt gacaaaatgc agcgtcatcc
    17041 cctcaataaa cgcatcaagc gcgcgggcgg ttccgggttc aaaccattgt tcgagcgttt
    17101 gctgactgcg ctgcatccag ttttgcatta ccgtttttaa tagcggtttt cggctagcca
    17161 gcgcgtagag ctggtacatc agctccatat tatccggcgt tgcaacctgt gagctgtaga
    17221 tcatatcggt gatagcctgg catgcgcctt gagcatcact aacatcgcta aaaaatgcct
    17281 gatattgccg ggacataatc tcagtaaaac tgctgaacgc ctccagcaac aactcatcaa
    17341 ttcctgaaaa atagtaggtc atcgatccca acggtacccc ggcaagggta gcaattttgc
    17401 ggtgcgtaac agcatgtatt ccgtaaagtt tcaccgcctc cagcgtggcc tggataattt
    17461 tttctcgccg ttgcggatcg ttagcgcgac gcatgtcatt tcctctgtct gcaattgtgt
    17521 acaaatgtac acaaccttgc taatgttgtc ttccctcttc ttattttgac gtgatctatg
    17581 accgtaaatt cttcacgtaa tgcattgaaa cgccgaacct gggcgctgtt tatgttcttc
    17641 tttttgccag gcctgttaat ggcgtcctgg gcaacccgta cgcctgctat ccgcgatatt
    17701 ctctctgtct cgatcgctga aatgggcggt gttctctttg gtctgtcgat cggttcgatg
    17761 agcggtattc tctgctcggc gtggttagtg aaacgctttg ggacacgtaa tgtcatcctg
    17821 gtcacgatgt cctgcgcatt gatcgggatg atgatattaa gtctggcact ctggctgaca
    17881 tcgcccctgc tctttgccgt tggtctcggc gtctttgggg caagttttgg ttctgcggaa
    17941 gtggcgataa acgttgaagg tgccgccgtt gagcgagaaa tgaataaaac ggttttgccg
    18001 atgatgcacg gtttttatag cctaggcacg ctggcaggcg ctggtgtcgg gatggcactg
    18061 acggcctttg gcgttccggc aacggtgcac attttattgg cggcgctggt aggtatcgcg
    18121 cctatttata tcgccattca ggcaatccct gacggtacgg gcaaaaatgc tgccgatggc
    18181 acccagcatg gcgaaaaagg cattcctttt tatcgcgata tccagttgct gttgattggt
    18241 gttgtagtgc tggcgatggc ctttgccgaa ggttctgcca acgactggtt acccttatta
    18301 atggttgacg gtcacggttt tagccctact tccggctcgc tgatttatgc cggttttacc
    18361 ctagggatga ccgttggacg cttcactggc ggttggttca tcgaccgtta cagtcgcgtt
    18421 gccgtggttc gggccagtgc actaatgggg gcgttgggta ttgggctgat tatttttgtc
    18481 gatagcgcct gggtcgctgg ggtgtctgtt gtactctggg gactgggtgc ctcgctgggc
    18541 ttcccgctga ccatttctgc cgccagcgat accggccccg atgcaccaac ccgcgtcagt
    18601 gtggtagcaa cgaccggtta tctggctttc ctcgtcgggc cgccgctgct gggctatctc
    18661 ggcgaacatt atggattacg tagtgcaatg ctggttgtac tggcgctggt tattctcgcg
    18721 gctattgtcg cgaaagccgt cgccaaaccc gataccaaaa cgcggacggc gatggagaat
    18781 agttgatgag cattaaatta attgcggtag acatggatgg tactttctta agcgatcaaa
    18841 aaacctataa ccgtgagcgg tttatggctc agtatcagca aatgaaagca caaggcattc
    18901 gctttgtggt cgccagcggg aatcaatatt atcagttgct ctctttcttc cctgaaattg
    18961 ctaatgaaat tgcctttgtg gctgaaaacg gcggctgggt agtgagtgaa ggcaaagatg
    19021 tttttaatgg cgagctgtcg aaggatgcct ttactactgt cgtggaacat ttgctgacgc
    19081 gcccggaagt ggaaattatt gcctgcggaa aaaatagcgc ctatacgctc aaaaaatatg
    19141 acgatgccat gaaaacggtg gcagaaatgt attatcaccg tctggaatac gtcgataact
    19201 ttgacaactt agaagatatt ttctttaagt ttggcctgaa tctttccgat gaactgattc
    19261 cacaagtaca aaaagcatta catgaggcca tcggcgatat tatggtgcca gtccacaccg
    19321 gcaacggcag catcgatctg attatccccg gcgtacataa agccaatggc cttcgccaac
    19381 tgcagaaatt atggggaata gacgacagcg aagtggtggt ctttggcgat ggcggtaacg
    19441 atattgagat gctgcgtcag gcaggcttta gttttgcaat ggaaaatgcc ggcagcgcgg
    19501 tcgtcgcagc ggcaaaatac cgggcaggct ccaataaccg tgaaggcgta ctggatgtga
    19561 tcgataaagt gcttaaacac gaagcgccat ttgaccaata aaaacggcag cacgcccggc
    19621 aacacaatac ctgaatgaag gctgcataat tcgcggaaat taacccgctg cattatttca
    19681 ggcgaaatga ttatgaaaaa ttgtctactg ttgggcgcgc ttttaatggg ctttactggt
    19741 gtggcgatgg cgcaaagtgt caccggcgat gtgccgagtg gatacaaagt ggttgtagtt
    19801 cctgattcag tcagcgttcc acaagcggtc agcgtcgcga ctgtgccgca aaccgtctat
    19861 gtggctcctg cgccagctcc ggcttaccgc ccgcatcctt atgctcgcca tcttgccagc
    19921 gtaggtgaag ggatggttat cgaacaccag attgacgacc atcaccatta attcgagaat
    19981 gcctgatcgc acaaagcagt caggcatttt tttacccttc gtgagaattt cccatctgtt
    20041 tatcttttaa aaagataacc atcagcgaca gccacaaaat tccgttgaca aggttgaaga
    20101 gattaaactg tccgttgccc ccgttcagcc aggcatgttt gctgatttca ataccaacgg
    20161 taaagatcag catttgcagc attcccatcg ccgcagaaac cgtaccttta ctcatatcgc
    20221 tggcaaacag ggttaatcgc accagtcccg cattcgccag accaataccg aaagcataaa
    20281 tacttaaccc ggcagtcatc cataaatacg cgtgcgatga gataaccgtt gccgcagcag
    20341 cgaccaacag accaatcata atcggccagc cgcccataat tatcacgtac ggtgcggcgc
    20401 gaggtcagac gcgctaacag caagttaccc gcaattaacg ccccgaaaat aggcacttgc
    20461 agcaagccat attcatagct gctcaactgc tccccggtaa tgatgataat cggcgactgg
    20521 gcgatccacg ccagcaatgg caggctaacg aatcccagcg ccagcgcccc cgccacaaag
    20581 cggccgttct tcagcaccag cttatagtca cgaccgagtt ctttcagtga cagtttctcg
    20641 cctatacgcg tggcggtttc aggcatagct cgttgcagac cgaaaaagga gatcgctgcc
    20701 aatgcggcaa acaaaacaaa catcccctcc cagggcagca catggatcca cgccgcgccc
    20761 accagcggac caagtagcgg agcaatcagc gccacgttcg ccatcagcgc ggtgatcttg
    20821 atacaaaccg cctcttcgaa ggattcctga attgcggcgt atcccacagc gccaatgaaa
    20881 cagaggctta tgccctgcaa gaagcgcaac agggtgaatt gttcaatgtt ttgcgccagt
    20941 aatattgcca gacaggtgac gataaaccac accacacccg ccagcatcac cggacggcga
    21001 ccaatacgat ccgacagcgg ccccaggagc cattgtaaaa acatcccgcc cgccagatac
    21061 gcggtcatcg aagtaggaac ccaatcaatg cccgcctgat attgttccac cacggccaac
    21121 ataccgggtt ggatcatatc gttgccgata taggttgaaa attcgtaaag caccagacag
    21181 agagggaaaa gtaacgcctg acgtccaagc ctggcaccga aagctaattt attttgcatg
    21241 caatttcttc gccaataata atcgcgcaga gtttaataaa agcgcagcta acgagaaagc
    21301 gaattttgta gctgaaacca cggttaagca cattcttaca ttattgcgag tatagctacg
    21361 ctttctttaa gttttattta acctatgccc gttacaatca cccaccgtaa acaggccgct
    21421 tgagggaaat aagacgatgc cgctttaccc agtttaacct gcactttatt ctcaacgact
    21481 tgcctgtatt ggctcccttt taatcacttt gcgtcgggaa gttatgctgg aaaatttgaa
    21541 tctctctcta ttctctctta ttaacgcgac gccagactcg gctccgtgga tgatctcgtt
    21601 ggcgattttt attgctaaag atttgattac cgtggtgccg ttgctggccg cggtactttg
    21661 gttgtggggg cttacagcac aacggcaact ggtgataaaa atcgctatcg cgctggcggt
    21721 cagcctgttt gtgtcctgga cgatgggaca tctttttccg cacgaccgac cctttgtcga
    21781 aaatatcggc tataacttcc tgcatcatgc ggcggatgac tcattcccga gcgatcacgg
    21841 tacggtgatt ttcacctttg cactggcatt tttatgctgg catcgcctgt ggtccggctc
    21901 acttttaatg gttctggccg tcgtcattgc ctggtcgcgc gtttatcttg gcgtccactg
    21961 gccgctggat atgctcggtg gattgctggc aggtatgatt ggctgcctta gtgcccagat
    22021 tatctggcaa gcgatggggc ataaactcta tcaacgtctg caatcgtggt atcgcgtctg
    22081 ttttgcatta ccgatccgca aaggctgggt gcgtgactga tctctgctaa aaagtgtagt
    22141 attgagcggc tcgcttcaat aactattcag agggattatg gaaacacgtc gcgaagagcg
    22201 tatcgggcag ctgctgcaag aattaaaacg cagcgataag ttacatctta aagacgccgc
    22261 cgccctgctt ggggtttcgg agatgacgat tcgtcgcgat ctgaacaacc acagtgcgcc
    22321 cgtcgttttg ctcggcggct atattgttct ggaaccgcgc agtgccagcc attacctgtt
    22381 aagcgatcaa aaatcccgcc tggtggaaga aaaacgccgg gcggcaaaac tggctgcgac
    22441 actggtagaa cccgatcaga ccctcttttt tgactgtggc accaccacgc cgtggattat
    22501 tgaagcgatt gataatgaaa tcccttttac cgccgtttgt tattcgctaa atacctttct
    22561 ggcgctgaaa gagaaacccc attgccgcgc atttctgtgc ggtggtgaat ttcacgccag
    22621 caacgccatt ttcaacccca tcgattttca gcaaacgttg aataattttt gcccggatat
    22681 cgctttttat tccgcggcgg gcgtgcatgt cagtaaaggc gctacctgtt ttaatcttga
    22741 agagttgccg gtaaaacact gggccatgtc gatggcgcaa aagcatgtgc tggttgtcga
    22801 ccacagtaaa tttggcaagg tgcgtccggc gcgcatgggt gacctgaaac gctttgatat
    22861 tgtggtgagt gattgttgcc cggaagatga gtatgtgaag tacgcgcaga cgcaccgtat
    22921 taagttgatg tattaatgac gtataaccgg atgacgtttc gcgccatccg gttatcagaa
    22981 gattaagaga accagctgcc gaaccactga tggaatttca tcatcacgaa atcccacatc
    23041 cgaccaaaga atccgccctc ttccacattt tccatcacga tcagcggacg ctgctcaatg
    23101 gatttaccgt taagctggaa atcaatggtc ccgacaacct gacctttttt cagcggtgcg
    23161 gtaagctgcg gttccgttaa cgtgtagctc gccttcaggt ttttcagctg cccacgcggt
    23221 atggtcacgg agcccgcttc gcctgccccg agattcactt cgctcttatc accaaaccag
    23281 acgcgctgag tcacaaaggt ggcatcaggt ttaattggcg tcacagtttc aaagaagcgg
    23341 aaaccccagg tcaataattt ctcagactca ttaaaacgga tacggtcagt tttcgccccc
    23401 agcactacgg agattaaacg catatcaccc tgggtagccg aagcaaccag attatatccc
    23461 gcacctgccg tggttcctgt cttcatgcca tcaacattca gattgctgct ccacagcaga
    23521 cggttacggt taggctgacg aattttgttg aaggtgaact ctttctcttt atgaatggcg
    23581 tactcttccg gcacatcgtg gatcaatgct ttacccagca atgccatatc gcgcgcggta
    23641 ctgaactgcc ccggcgcatc cagaccgtgc accgtctgga aggtagtgtt ggtcagaccc
    23701 agttttttgg cataaccatt catcaaacca ataaatgact cctggctccc ggcaacgtaa
    23761 tcggccagcg caatacaggc gtcattaccg gactggataa tcacaccttt gttcaagtct
    23821 gccaccgaaa cctgatcgcc cggtttgagg aacattaccg atgaaccacg cagtgccgga
    23881 tttcccgtcg cccaggcatc tttaccgacc gtcaccatat cggtgagttt aatcttatcg
    23941 gccttaagcg cctgcccaac cacatagctg gtcatgattt tagtcaggct cgcgggatcc
    24001 agtttctcat ccgcgttgcc ttctgccagc actttaccgc tggcgtaatc cattaaaatc
    24061 catgcacgcg catccacgct cggcgcttca acggtttgtt ccgccgcgaa tgccgttggg
    24121 gcaaaaagga ataaaaatgc agaacccgct gcaagaccac gaaggagaga ggagtattgc
    24181 gtcataaatg ccacccgagt atccattcaa aaaaaatacg ttatatcgcc gtatgataag
    24241 ttcggtgatt aataacgcac acacaaactt aaagaaaccg taagttggta aagtttttaa
    24301 agtttatgca atatcctgcc gcttcgctgc ttctggtgtc atttttttgc cagctgttgc
    24361 ttaaaagtta actttacttc accatcggag tcctgttcag cctgtggctg cctgtcggaa
    24421 aaggggtgag tatgattacg ctgtggggtc ggaataattc aactaacgta aaaaagtatt
    24481 gctgacgctc gaagaactgg aactacctta tgagcaaatt ctcgcgggcc gtgagtttgg
    24541 gataaatcac gatgctgatt ttctggcgat gaaccctaac gggctggtgc cgttgttgcg
    24601 tgacgacgaa agtgatctca ttctttggga atcaaacgcc attgtccgct atctggcggc
    24661 acagtacggg caaaaacgcc tgtggatcga ctcaccggca cgtcgtgcgg aagcggaaaa
    24721 atggatggac tgggcaaacc agacgctcag taatgctcat cgcgggatcc tgatgggatt
    24781 agtcagaaca ccaccggaag agcgcgatca ggccgccatt gatgccagtt gcaaagagtg
    24841 cgacgccctg tttgccctgc tcgatgcgga actggcaaaa gtaaaatggt tctctggcga
    24901 cgagtttggt gtgggcgata tcgctatcgc accgttcatc tacaatttgt ttaacgttgg
    24961 cctgacctgg acaccgcgtc caaatctgca acgctggtat cagcaactca ctgaacgccc
    25021 cgcagtgcgc aaagtggtga tgattcccgt tagctaattg cgtgggctaa ctttaagtaa
    25081 ttccccactg gactcgtcgg tgagaacgta taaataaccg tcgggtccag tgcgaacatc
    25141 acgaattcgc tgccctctgt ccgttaaaat acggccatct tctgtcactt tgtcgccgtt
    25201 gacgctcatc acaatgacat ctttatcttt cagcgcgcca ataaataatt tttgctgcca
    25261 ctgggggaat ttatcgctgt tatagaaggc catgccgctc acagcgggcg aatctttcca
    25321 gtaaaaaaca ggttgctcgg tcccggcgac gatctcccct ttcgcttccg gtatcttaaa
    25381 gcctgaatag ttgattcccc aggttgccag cggccagccg tagtttttgc ctttttgcgg
    25441 gatattaatt tcatcaccac cgcgcgggcc atgttcattc agccacagtg cattactcca
    25501 cggattcatc gccattcctt gcggattacg aatgccataa gaccagatct cggcgcgcgc
    25561 accggattcc tttataaaag gattatcatc cgggatttcg ccctggtcgg tcagacgcac
    25621 cagtttgccc tgtaatttat ccagatcctg cgccgtcggg cgctgattgt tttcgcccag
    25681 agcaataaaa agataacctt taccgtcgaa taccagccgc ccgccaaaat ggttgccggt
    25741 agacagtttt ggcatctggc ggaagacggt gcggaagtcg gtcacttttg agagatcatc
    25801 acttaagcgg ccataaccca cagcagttcc ggctttgcca tcatcgccaa cttcggaata
    25861 acttaaccag atgcggcgag actgagcaaa atcaggcgct aaaaccacgt ccagcaggcc
    25921 gccctgcccg tgcgcccaaa cgtccggaac tccggaaagc ggcgcagata atccttttcc
    25981 tgcttgccag tgacgcaact cgccgccgcg cagagtgatt aacataccgt gattatcggg
    26041 taaaaaggcc agtgcccagg gatggtcgag tttgtcttgc agtacttcga catttaccgt
    26101 tgcaggagcc gcccagagag cggaagaaag acaaataagg ggcacaagga aaaaggattg
    26161 tcgatgcata acgcctccgg attgactctg gcttaaagcg tagtcagtgg aggagataac
    26221 aaattcattt ttacaaaaac ttaaacatga agggggagac gctttctccc ccttagtttt
    26281 caggccttct caagcatggc gtgcttctgc aggctctgga tactcagcgt taagctcatc
    26341 agacaatttt caagcttatc ggcgttgacg gtaataacag tcgggcaatc atggtgccca
    26401 ctcatcaaac atactgcggc tgtcgctaat gcttcttcag catgatgaag agcactccac
    26461 tcttcctgat ccagatgaag attcaaccgc agcgatttat cgtgcagttc gcgattcagt
    26521 ttaaaaaagt tatctcgtag atgattgctt tcgctgacgg acatgtatcc ttttgccttc
    26581 ctcagttgca cgtatgcagc gaggtcaacg cgtgcgtcga tcaaccggtt cagcagatcg
    26641 attcgctgtc tgtcaacgaa catacttcga tcctcctctt tccagccttg ttgcgattaa
    26701 atagtatggc tattttttga gcaaaatgct gtccaggatc aattcttcac gccaaattaa
    26761 caaaatttac atttccttcg cctccgccag cgaatgagat tgcgatacaa attttatcca
    26821 gaactctgta gaatccctgc cctgaccttt cctgtaactg aatatttttt aagctatgag
    26881 caaagtaact ccccagccga aaatcggctt tgtttccctt ggctgtccga aaaaccttgt
    26941 cgattcagag cgtattctca ccgaactacg cactgaaggt tatgacgtgg taccgagcta
    27001 tgacgatgcg gacatggtga tcgtcaacac ctgcggcttt attgacagcg cggtacaaga
    27061 atcactggaa gccattggtg aagcgttgaa tgaaaacggc aaggtaattg tgaccggttg
    27121 tctgggggca aaagaagatc agatccgcga agtccacccg aaagtgctgg aaatcaccgg
    27181 gcctcatagc tatgagcagg ttctggagca cgttcatcac tacgtgccaa aaccgaaaca
    27241 caacccattc ctgagcctgg tgccagaaca aggtgtgaaa ctgacaccgc gtcattatgc
    27301 ctatctgaaa atttcggaag gctgtaatca ccgctgcacc ttctgcatta ttccgtctat
    27361 gcgcggcgac ctggtgagcc gtccgattgg cgaagtgtta agtgaagcga aacgtctggt
    27421 agatgcgggc gttaaagaga ttctggtgat ctcgcaggat acttccgcct atggcgttga
    27481 tgttaaacat cgtactggct tccacaacgg cgagccggta aaaaccagca tggtcagcct
    27541 gtgcgaacag ttatcgaaac tggggatctg gacacgtctg cactacgttt acccttatcc
    27601 gcatgtggac gacgtcatcc cactgatggc agaaggcaaa atcctgccgt atctggacat
    27661 tccgttgcag cacgccagcc cgcgcattct caaactgatg aagcgtccgg gttctgtaga
    27721 tcgccaactg gcgcgcatca aacagtggcg cgaaatctgc ccggaactga ccctacgctc
    27781 aacctttatt gtcggcttcc ctagcgagac ggaagaagat ttccagatgc tactcgactt
    27841 cctgaaagaa gcgcgtctgg atcgcgttgg ctgctttaaa tacagcccgg ttgaaggtgc
    27901 agacgccaat gccctgcctg accaggttcc ggaagaagtg aaagaagaac gctggaaccg
    27961 tttcatgcag ctgcagcagc agatttccgc cgagcgcctg caagagaaag tgggccgtga
    28021 aattctggtg attatcgacg aagtggacga agaaggcgcg attggtcgca gcatggcaga
    28081 tgcaccggaa atcgacggcg cggtttatct caatggtgaa accaacgtta agccgggtga
    28141 tatcctgcgt gtgaaagtcg agcacgccga tgagtacgat ttgtggggta gccgggttta
    28201 attaattcag gccgggcaaa atcatttgtc cgccctgcga ataatgatta ctgccagcta
    28261 atctgagccc tgccgttttt cttactctta tacagccgct catccgccag cattttcaag
    28321 cgttcgacgg tggtacattc atttgaatgc gctacaccga tactggtact taaattaatg
    28381 cttcccacct ggaaatgaac gatttctttt agaatcgata gcaactcttc cagcgttcca
    28441 cccgtataaa tcaccgcaaa ttcgtcaccg ccgaagcggt agattttacc tttatctacc
    28501 gggatctgtg atttaaggat tttcaccact ttcatcaaca cttcatcgcc cagcaaatga
    28561 ccgaaggtgt cgttaatgtt tttgaattta tcgacgtcaa acaggcacaa ataaccgcct
    28621 ttttccggca gcgcttttaa atcttcgtca aaggattttc tgcccattgc tttggtcagg
    28681 tcgtccagga acgccgagtt atacatactg tagagttttt tgacatcggc agccgaaaag
    28741 aaaagcccgc catccagtac gcgttcaaaa tggttattaa tgatggcttc tttctccaca
    28801 ataccacgat taatcactct gcgtaccaga tagaggcaac tggtacccga tatcactgcc
    28861 accaatgcgg caaagaaaat aatcaatgta cagacccgca aataaacatc ttttttatac
    28921 gcaaatacgt acaatccggt cggcaaatag gcgaagtgct ccatcgcttc cgaaacgatg
    28981 aagatggcgt ttttagcatt atattcatca ccggtttacc gttcttaaat agagagaaca
    29041 tcaggttctc gctgtccatc gccagcgatt ttgacagtaa agaaaaacgc tccagaccaa
    29101 gcaaaaatat cagactacga ttgtgagtta ccggatagag gtaatagacg aaggttttac
    29161 cgtcaaaaga gataaaccct gtatttttga catgtcgata cttcacaacc tgcataatat
    29221 ctgcacaata atttttctcg aactccttta cctgaataca ttcgtcactg gaaatcagat
    29281 aaggaccgac atcgtacaaa taagaggggg aaaccgagtc ggcgtaagct gtgcgcaaac
    29341 caaccaacag ctgttcctcc gtgggattta gcgtgttgat aagatcaatt gccacgctac
    29401 gccgatattc agcaatattt gccaccgcaa aagcagtatc aataacggac tgtttcaggc
    29461 tatacacccg ttcctttacc atttgagtgt agatcccgca ggcaactgct gaaatcatga
    29521 taaaaatcag cagactgact gtaagtacga acttattgat tctggacata cgtaaactac
    29581 tcttttacta attttccact tttatcccag gcggagaatg gcatgggttt ctggtacaga
    29641 tacccctgag cctgaacaca tcctaattta attaattcgc gcgcaacgtc ttcgctctct
    29701 acgccttccg caatcaccgg gatccggtta tagcgtgcca gatcgaaaat aatgcgcaca
    29761 atttttcccg cagtaccgaa aatatcgccg gtaaattgcg agtccacctt tatgctgtct
    29821 ggattaattt cataaaaacg tttcaggttt gacaaaccgg tgccaaagtc atcaatcgat
    29881 atccgtaccc cttgtttacg cagttgttca atattgagat aaaaatgttc attaattcgc
    29941 tcgatcacgg tttcagtgat ctcaacacag aggctgttcg ccttcaggcc attttctttc
    30001 atgatggtgg taaaacgctc atgaaagtgc ggttcgttta actgccaggg cgaaacatta
    30061 atatgcagta agaaatcatc cttcagcgac gcacgctgac taaaatgtct gaattcaccc
    30121 attgccgttt taataatctg ataacccagc tcattgataa acccgatatc ttcagcaatg
    30181 gtaatgaact tcagcggtga aatgatcccc cgctccgcag aaacccaacg gcacaacgcc
    30241 tccgcgccca gaatggcgcg ggtgttaata tcaacgatcg gttgatagac gaggaatagt
    30301 tcgccaattt ccattgcatg gcgaatatta cgcgcaatcc agatattctc ttctatttcg
    30361 ttgagtttcg cgtcatcaca gaaaataaac tcggagaaca tatggtcacg gatactggaa
    30421 atcgccagtt tcagtttttc tgcgtaactc caggcgtttt ccccctgcaa aggttcacaa
    30481 atggcagact tatggatgcg cagattgcgg tagccaaagc tgctgtcaaa atcgtttacc
    30541 atcgctattt tacgggtaaa tgtctgtaca ttttcccgac acagaagtaa atagagatcg
    30601 gcattgaatg tcacgatatc gcagcattgc gcatatttct cggcaattcg ctgacacaat
    30661 ttcctgacga ttttattgcg ttcatcaaca ccaaacaccg cttccacggc ttccagacca
    30721 cacagtttga tgatccccac agccagatac tgattattgt tggactcaat aatcgccata
    30781 tttttaataa aaccgctttc ctgatcgtaa gagatcagtg agagcaattt atttgaccgt
    30841 tcaatttcgg catctttcat ttccgcgatc gaa
//
LOCUS       DAZPDU010000035        30154 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZPDU010000035 DAZPDU010000000
VERSION     DAZPDU010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30154
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.035"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..231
                     /gene="tuf"
                     /locus_tag="ACHURS_001438"
     CDS             <1..231
                     /gene="tuf"
                     /locus_tag="ACHURS_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFR1237211.1"
                     /translation="RHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVT
                     LIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            461..844
                     /gene="secE"
                     /locus_tag="ACHURS_001439"
     CDS             461..844
                     /gene="secE"
                     /locus_tag="ACHURS_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFR1237212.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            846..1391
                     /gene="nusG"
                     /locus_tag="ACHURS_001440"
     CDS             846..1391
                     /gene="nusG"
                     /locus_tag="ACHURS_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFR1237213.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1550..1978
                     /gene="rplK"
                     /locus_tag="ACHURS_001441"
     CDS             1550..1978
                     /gene="rplK"
                     /locus_tag="ACHURS_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFR1237214.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1982..2686
                     /gene="rplA"
                     /locus_tag="ACHURS_001442"
     CDS             1982..2686
                     /gene="rplA"
                     /locus_tag="ACHURS_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFR1237215.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3099..3596
                     /gene="rplJ"
                     /locus_tag="ACHURS_001443"
     CDS             3099..3596
                     /gene="rplJ"
                     /locus_tag="ACHURS_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFR1237216.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3663..4028
                     /gene="rplL"
                     /locus_tag="ACHURS_001444"
     CDS             3663..4028
                     /gene="rplL"
                     /locus_tag="ACHURS_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFR1237217.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKSLEEAGAEVEVK"
     gene            4348..8376
                     /gene="rpoB"
                     /locus_tag="ACHURS_001445"
     CDS             4348..8376
                     /gene="rpoB"
                     /locus_tag="ACHURS_001445"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFR1237218.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8453..12676
                     /gene="rpoC"
                     /locus_tag="ACHURS_001446"
     CDS             8453..12676
                     /gene="rpoC"
                     /locus_tag="ACHURS_001446"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFR1237219.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12918..14051)
                     /gene="thiH"
                     /locus_tag="ACHURS_001447"
     CDS             complement(12918..14051)
                     /gene="thiH"
                     /locus_tag="ACHURS_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFR1237220.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14048..14818)
                     /gene="thiG"
                     /locus_tag="ACHURS_001448"
     CDS             complement(14048..14818)
                     /gene="thiG"
                     /locus_tag="ACHURS_001448"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFR1237221.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAEMLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(14820..15020)
                     /gene="thiS"
                     /locus_tag="ACHURS_001449"
     CDS             complement(14820..15020)
                     /gene="thiS"
                     /locus_tag="ACHURS_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFR1237222.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15004..15759)
                     /gene="thiF"
                     /locus_tag="ACHURS_001450"
     CDS             complement(15004..15759)
                     /gene="thiF"
                     /locus_tag="ACHURS_001450"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFR1237223.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVVLNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15752..16387)
                     /gene="thiE"
                     /locus_tag="ACHURS_001451"
     CDS             complement(15752..16387)
                     /gene="thiE"
                     /locus_tag="ACHURS_001451"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFR1237224.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     NEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16387..18282)
                     /gene="thiC"
                     /locus_tag="ACHURS_001452"
     CDS             complement(16387..18282)
                     /gene="thiC"
                     /locus_tag="ACHURS_001452"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFR1237225.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(18363..18463)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18516..18992)
                     /locus_tag="ACHURS_001453"
     CDS             complement(18516..18992)
                     /locus_tag="ACHURS_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rsd/AlgQ family anti-sigma factor"
                     /protein_id="HFR1237226.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKLGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19087..19860
                     /gene="nudC"
                     /locus_tag="ACHURS_001454"
     CDS             19087..19860
                     /gene="nudC"
                     /locus_tag="ACHURS_001454"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFR1237227.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPLSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19900..20964
                     /gene="hemE"
                     /locus_tag="ACHURS_001455"
     CDS             19900..20964
                     /gene="hemE"
                     /locus_tag="ACHURS_001455"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFR1237228.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20974..21645
                     /gene="nfi"
                     /locus_tag="ACHURS_001456"
     CDS             20974..21645
                     /gene="nfi"
                     /locus_tag="ACHURS_001456"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFR1237229.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLMVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21688..22278
                     /locus_tag="ACHURS_001457"
     CDS             21688..22278
                     /locus_tag="ACHURS_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFR1237230.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            22465..22737
                     /gene="hupA"
                     /locus_tag="ACHURS_001458"
     CDS             22465..22737
                     /gene="hupA"
                     /locus_tag="ACHURS_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFR1237231.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22750..23445
                     /locus_tag="ACHURS_001459"
     CDS             22750..23445
                     /locus_tag="ACHURS_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFR1237232.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALLLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23447..23872)
                     /gene="zraP"
                     /locus_tag="ACHURS_001460"
     CDS             complement(23447..23872)
                     /gene="zraP"
                     /locus_tag="ACHURS_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFR1237233.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24110..25486
                     /gene="zraS"
                     /locus_tag="ACHURS_001461"
     CDS             24110..25486
                     /gene="zraS"
                     /locus_tag="ACHURS_001461"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFR1237234.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLDAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGATFTLWLPVT
                     ITRKGPQG"
     gene            25483..26808
                     /gene="zraR"
                     /locus_tag="ACHURS_001462"
     CDS             25483..26808
                     /gene="zraR"
                     /locus_tag="ACHURS_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFR1237235.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADRRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAVHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIAGTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26805..28094)
                     /gene="purD"
                     /locus_tag="ACHURS_001463"
     CDS             complement(26805..28094)
                     /gene="purD"
                     /locus_tag="ACHURS_001463"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFR1237236.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGMKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVAGGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28106..29695)
                     /gene="purH"
                     /locus_tag="ACHURS_001464"
     CDS             complement(28106..29695)
                     /gene="purH"
                     /locus_tag="ACHURS_001464"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFR1237237.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7056 a         7867 c         8307 g         6924 t
ORIGIN      
        1 cgtcatactc cgttcttcaa aggctaccgt ccgcagttct acttccgtac tactgacgtg
       61 actggtacca tcgaactgcc ggaaggcgta gagatggtaa tgccgggcga caacatcaaa
      121 atggttgtta ccctgatcca cccgatcgcg atggacgacg gtctgcgttt cgcaatccgt
      181 gaaggcggcc gtaccgttgg cgcgggcgtt gtagcaaaag ttctgagcta attgccgata
      241 acatttgacg caatgcgcac taaaagggca tcatttgatg ccctttttgc acgctttcgt
      301 accagaacct ggctcatcag tgattttctt tgtcataatc attgctgaga caggctctgt
      361 tgagggcgta taatccgaaa agctaatacg cgtttcgatt tggtttgcct cgcgatcgcg
      421 gggtgaaaat gtttgtagaa aacttctgac aggttggttt atgagtgcga ataccgaagc
      481 tcaaggaagc gggcgcggcc tggaagcgat gaagtgggtc gttgtggtgg cattgctcct
      541 ggtggcgatt gtcggcaact acctttatcg cgacattatg ctgccgctgc gtgcgctggc
      601 cgtagtaatt ctgattgctg cagcgggtgg tgtcgcgctg ttaacgacaa aaggtaaagc
      661 taccgttgct tttgcccgtg aagcgcgtac cgaagtccgt aaggtcattt ggccgactcg
      721 ccaggaaaca ttgcacacca cgctgattgt ggctgcggtt accgcagtaa tgtcactgat
      781 cctgtgggga ctggatggta ttctggttcg cctggtatcc tttatcactg gcctgaggtt
      841 ctgagatgtc tgaagctcct aaaaagcgct ggtacgtcgt tcaggcgttt tccggttttg
      901 aaggccgcgt agcaacgtcg ctacgtgagc atatcaaatt acacaacatg gaagatttgt
      961 ttggtgaagt catggtacca accgaagaag tggttgaaat ccgtggcggt cagcgtcgca
     1021 aaagcgaacg taaattcttc cctggctacg tccttgttca gatggtgatg aacgacgcga
     1081 gctggcacct ggtgcgcagc gtaccgcgtg tgatgggctt catcggcggt acttccgatc
     1141 gtcctgcgcc aatcagcgat aaagaagtcg atgcgattat gaaccgcctg cagcaggttg
     1201 gtgataagcc gcgtccgaaa acgctgtttg aaccgggtga aatggtccgt gttaatgatg
     1261 gtccgttcgc tgacttcaac ggtgttgttg aagaagtgga ttacgagaaa tctcgtctga
     1321 aagtgtctgt ttctatcttc ggtcgtgcga ccccggtaga gctggacttc agccaggttg
     1381 aaaaagccta acccagcgat caaaaaagcg gcgatttaat cgttgcacaa ggcgtgagat
     1441 tggaatacaa tttcgcgcct tttgttttta tgggccttgc ccgtaaaacg attttttata
     1501 tcacggggag cctctcagag gcgttattac ccaacttgag gaatttataa tggctaagaa
     1561 agtacaagcc tatgtcaagc tgcaggttgc agctggtatg gctaacccga gtccgccagt
     1621 aggtccggct ctgggtcagc agggcgtaaa catcatggaa ttctgcaaag cgttcaacgc
     1681 aaaaactgat tccatcgaaa aaggtctgcc gattccggta gtaatcaccg tttacgctga
     1741 ccgttctttc actttcgtta ccaagactcc gccggcagca gttctgctga aaaaagcggc
     1801 tggtatcaag tctggttccg gtaagccgaa caaagacaaa gtgggtaaaa tttcccgcgc
     1861 tcagctgcag gaaatcgcgc agaccaaagc tgccgacatg actggtgccg acattgaagc
     1921 gatgactcgc tccatcgaag gtactgcacg ttccatgggc ctggtagtgg aggactaaga
     1981 aatggctaaa ctgaccaagc gcatgcgtgt tatccgcgag aaagttgatg caaccaaaca
     2041 gtacgacatc aacgaagcta tcgcactgct gaaagagctg gcgactgcta aattcgtaga
     2101 aagcgtggac gtagctgtta acctcggcat cgacgctcgt aaatctgacc agaacgtacg
     2161 tggtgcaact gtactgccgc acggtactgg ccgttccgtt cgcgtagccg tatttaccca
     2221 aggtgcaaac gctgaagctg ctaaagctgc aggcgcagaa ctggtaggta tggaagatct
     2281 ggctgaccag atcaagaaag gcgaaatgaa ctttgacgtt gttattgctt ctccggatgc
     2341 aatgcgcgtt gttggccagc tgggccaggt tctgggtccg cgcggcctga tgccaaaccc
     2401 gaaagtgggt actgtaacgc cgaacgttgc tgaagcggtt aaaaacgcta aagctggcca
     2461 ggttcgttac cgtaacgaca aaaacggcat catccacacc accatcggta aagtggactt
     2521 tgacgctgac aaactgaaag aaaacctgga agctctgctg gttgcgctga aaaaagcaaa
     2581 accgactcag gcgaaaggcg tgtacatcaa gaaagttagc atctccacca ccatgggtgc
     2641 aggtgttgca gttgaccagg ctggcctgag cgcttctgta aactaatgcc tttacgtggg
     2701 cggtgatttt gtctacaatc ttacccccac atataatgct taatgcagac gtatatccga
     2761 gatattcggg ttgtggcaag gcggcaactg agtgagtcgc caggagcata gctaactatg
     2821 tgactggtgc gaatgaagga agccaacgcc gccacaagtt gaatagcgac ggatagaaaa
     2881 gatttgttcg ttggagcctg gcctatccag gcctccgtcg aagaccgcag gagtttcgca
     2941 agaaacttaa tcccctgcgt agacggtgac agaacgctaa gattattctt ttatattctg
     3001 gcttgtttct gctcaccgta attaagacgc tctctccgct tggaggagtg aagtgagttc
     3061 cagagatttt ctctggcaaa catccaggag caaagctaat ggctttaaat cttcaagaca
     3121 aacaagcgat tgttgctgaa gtcagcgaag tagccaaagg cgcgctgtct gcagtagttg
     3181 cggattcccg tggcgtaact gtagataaaa tgactgaact gcgtaaagca ggtcgcgaag
     3241 ctggcgtata catgcgtgtt gttcgtaaca ccctgctgcg ccgtgctgtt gaaggtactc
     3301 cgttcgagtg cctgaaagac gcgtttgttg gtccgaccct gattgcatac tctatggaac
     3361 acccgggcgc tgctgctcgt ctgttcaaag agttcgcgaa agcgaatgca aaatttgagg
     3421 tcaaagccgc tgcctttgaa ggtgagctga tcccggcgtc tcagatcgac cgcctggcaa
     3481 ctctgccgac ctacgaagaa gcaattgcac gcctgatggc aaccatgaaa gaagcttcgg
     3541 ctggtaaact ggttcgtact ctggctgctg tacgcgatgc gaaagaagct gcttaatcgc
     3601 agttatcttt ttaacgcatt cgcttacgta taaacttatt ctgatattca ggaacaattt
     3661 aaatgtctat cactaaagat caaatcattg aagcagttgc agctatgtct gtaatggacg
     3721 ttgtagaact gatctctgca atggaagaaa aattcggtgt ttccgctgct gctgctgtag
     3781 ctgtagctgc tggcccggtt gaagctgctg aagaaaaaac tgaattcgac gtaattctga
     3841 aagctgctgg cgctaacaaa gttgctgtta tcaaagcagt acgtggcgca actggcctgg
     3901 gtctgaaaga agctaaagac ctggtagaat ctgcaccggc tgctctgaaa gaaggcgtga
     3961 gcaaagacga cgcagaagca ctgaaaaaat ccctggaaga agctggcgct gaagttgaag
     4021 ttaaataagc caacccttcc ggttgcagcc tgagaaatca ggctgatggc tggtgacttt
     4081 ttggtcacca gcctttttgc gctgtaaggc gccagtagcg tttcacactg tttgactact
     4141 gctgtgcctt tcaatgcttg tttctatcga cgacttaata tactgcgaca ggacgtccgt
     4201 tctgtgtaaa tcgcaatgaa atggtttaag cgtgatagca acaggcattg cggaaagtgt
     4261 tccattttcc ggtcaacaaa atagtgttgc acaaactgtc cgctcaatgg acagatgggt
     4321 cgacttgtca gcgagctgag gaaccctatg gtttactcct ataccgagaa aaaacgtatt
     4381 cgtaaggatt ttggtaaacg tccacaagtt ctggatgtac cttatctcct ttctatccag
     4441 cttgactcgt ttcagaaatt tatcgagcaa gatcctgaag ggcagtatgg tctggaagct
     4501 gctttccgtt ccgtattccc gattcagagc tacagcggta attccgagct gcaatacgtc
     4561 agctaccgcc ttggcgaacc ggtgtttgac gtccaggaat gtcaaatccg tggcgtgacc
     4621 tattccgcac cgctgcgcgt taaactgcgt ctggtgatct atgagcgcga agcgccggaa
     4681 ggcaccgtaa aagacattaa agaacaagaa gtctacatgg gcgaaattcc gctcatgaca
     4741 gacaacggta cctttgttat caacggtact gagcgtgtta tcgtttccca gctgcaccgt
     4801 agtccgggcg tcttctttga ctccgacaaa ggtaaaaccc actcttcggg taaagtgctg
     4861 tataacgcgc gcatcatccc ttaccgtggt tcctggctgg acttcgaatt cgatccgaag
     4921 gacaacctgt tcgtacgtat cgaccgtcgc cgtaaactgc ctgcgaccat cattctgcgc
     4981 gccctgaact acaccacaga gcagatcctc gacctgttct ttgaaaaagt tatctttgaa
     5041 atccgtgata acaagctgca gatggagctg gtgccggaac gcctgcgtgg tgaaactgca
     5101 tctttcgaca tcgaagctaa cggtaaagtg tacgtagaaa aaggccgccg tatcactgcg
     5161 cgccacattc gccagctgga aaaagacgac gtcaaactga tcgaagtccc ggttgagtac
     5221 atcgcaggta aagtggttgc taaagactat attgatgagt ctaccggcga gctgatctgc
     5281 gcagcgaaca tggagctgag cctggatctg ttggctaagc tgagccagtc tggtcacaag
     5341 cgtattgaaa cgctgttcac caacgacctg gatcacggcc cgtatatctc tgaaacctta
     5401 cgtgtcgacc caactaacga ccgtctgagc gcactggtag aaatctaccg catgatgcgc
     5461 cctggcgagc cgccgactcg tgaagcagct gaaagcctgt tcgagaacct gttcttctcc
     5521 gaagaccgtt atgacctgtc tgcggttggt cgtatgaagt tcaaccgttc tctgctgcgc
     5581 gaagaaatcg aaggttccgg tatcctgagc aaagacgaca tcattgatgt tatgaaaaag
     5641 ctcatcgata tccgtaacgg taaaggcgaa gtcgatgata tcgaccacct cggcaaccgt
     5701 cgtatccgtt ctgttggcga aatggcggaa aaccagttcc gcgttggcct ggtacgtgta
     5761 gagcgtgcgg tgaaagagcg tctgtctctg ggcgatctgg ataccctgat gcctcaggac
     5821 atgatcaacg ccaagccgat ttccgcagca gtgaaagagt tcttcggttc cagccagctg
     5881 tctcagttta tggaccagaa caacccgctg tctgagatta cgcacaaacg tcgtatctcc
     5941 gcactcggcc cgggtggtct gacccgtgaa cgtgcaggct tcgaagttcg agacgtacac
     6001 ccgactcatt acggtcgcgt atgtccaatc gaaacccctg aaggtccgaa catcggtctg
     6061 atcaactctt tgtccgtgta cgcacagact aacgaatacg gcttccttga gactccgtat
     6121 cgtaaggtga ccgacggtgt tgtaactgac gaaattcact acctttctgc tatcgaagaa
     6181 ggcaactacg ttatcgccca ggcgaactcc aacctggatg aagaaggcca cttcgtagaa
     6241 gacctggtaa cttgccgtag caaaggcgaa tccagcttgt tcagccgtga ccaggttgac
     6301 tacatggacg tatccaccca gcaggtggta tccgtcggtg cgtccctgat cccgttcctg
     6361 gaacacgatg acgccaaccg tgcattgatg ggtgcgaaca tgcaacgtca ggccgttccg
     6421 actctgcgcg ctgataagcc gctggttggt actggtatgg aacgtgctgt tgccgttgac
     6481 tccggtgtaa ctgcggtagc taaacgtggt ggtgtcgttc agtacgtgga tgcttcccgt
     6541 atcgttatca aagttaacga agacgagatg tatccgggtg aagcaggtat cgacatctac
     6601 aacctgacca aatacacccg ttctaaccag aacacctgta tcaaccagat gccgtgtgtg
     6661 tctctgggtg aaccggttga acgtggcgac gtgctggcag acggtccgtc caccgacctc
     6721 ggtgaactgg cgcttggtca gaacatgcgc gtagcgttca tgccgtggaa tggttacaac
     6781 ttcgaagact ccatcctcgt atccgagcgt gttgttcagg aagaccgttt caccaccatc
     6841 cacattcagg aactggcgtg tgtgtcccgt gacaccaagc tggggccgga agagatcacc
     6901 gctgacatcc cgaacgtggg tgaagctgcg ctctccaaac tggatgaatc cggtatcgtt
     6961 tacattggtg cggaagtgac cggtggcgac attctggttg gtaaggtaac gccgaaaggt
     7021 gaaactcagc tgaccccaga agaaaaactg ctgcgtgcga tcttcggtga gaaagcctct
     7081 gacgttaaag actcttctct gcgcgtacca aacggtgtat ccggtacggt tatcgacgtt
     7141 caggtcttta ctcgcgatgg cgtagaaaaa gacaaacgtg cgctggaaat cgaagaaatg
     7201 cagctcaaac aggcgaagaa agacctgtct gaagaactgc agatcctcga agcgggtttg
     7261 ttcagccgta tccgtgctgt gctggtagcc ggtggcgttg aagctgagaa gctcgacaaa
     7321 ctgccgcgcg atcgctggct ggagctgggc ctgaccgacg aagagaaaca aaatcagctg
     7381 gaacagctgg ctgagcagta tgacgaactg aaacacgagt tcgagaagaa actcgaagcg
     7441 aaacgccgca aaatcaccca gggcgacgat ctggcaccgg gcgtgctgaa gattgttaag
     7501 gtgtatctgg cggttaaacg ccgtatccag cctggtgaca agatggcagg tcgtcacggt
     7561 aacaagggtg taatttctaa gatcaacccg atcgaagata tgccttacga tgaaaacggt
     7621 acgccggtag acatcgtact gaacccgctg ggcgtaccgt ctcgtatgaa catcggtcag
     7681 atcctcgaaa cccacctggg tatggctgcg aaaggtatcg gcgacaagat caacgccatg
     7741 ctgaaacagc agcaagaagt cgcgaaactg cgcgaattca tccagcgtgc gtacgatctg
     7801 ggcgctgacg ttcgtcagaa agttgacctg agtaccttca gcgatgaaga agttatgcgt
     7861 ctggctgaaa acctgcgcaa aggtatgcca atcgcaacgc cggtgttcga cggtgcgaaa
     7921 gaagcagaaa ttaaagagct gctgaaactt ggcgacctgc cgacttccgg tcagatccgc
     7981 ctgtacgatg gtcgcactgg tgaacagttc gagcgtccgg taaccgttgg ttacatgtac
     8041 atgctgaaac tgaaccacct ggtcgacgac aagatgcacg cgcgttccac cggttcttac
     8101 agcctggtta ctcagcagcc gctgggtggt aaggcacagt tcggtggtca gcgtttcggg
     8161 gagatggaag tgtgggcgct ggaagcatac ggcgcagcat acaccctgca ggaaatgctc
     8221 accgttaagt ctgatgacgt gaacggtcgt accaagatgt ataaaaacat cgtggacggc
     8281 aaccatcaga tggagccggg catgccagaa tccttcaacg tattgttgaa agagattcgt
     8341 tcgctgggta tcaacatcga actggaagac gagtaattct cgctcaaaca ggtcactgct
     8401 gtcggggtaa aacccggcag cggattgtgc taactccgac gggagcaaat ccgtgaaaga
     8461 tttattaaag tttctgaaag cgcagactaa aaccgaagag tttgatgcga tcaaaattgc
     8521 tctggcttcg ccagacatga tccgttcatg gtctttcggt gaagttaaaa agccggaaac
     8581 catcaactac cgtacgttca aaccagaacg tgacggcctt ttctgcgccc gtatctttgg
     8641 gccggtaaaa gattacgagt gcctgtgcgg taagtacaag cgcctgaaac accgtggcgt
     8701 catctgtgag aagtgcggcg ttgaagtgac ccagactaaa gtacgccgtg agcgtatggg
     8761 ccacatcgaa ctggcttccc cgactgcgca catctggttc ctgaaatcgc tgccgtcccg
     8821 tatcggtctg ctgctcgata tgccgctgcg cgatatcgaa cgcgtactgt actttgaatc
     8881 ctatgtggtt atcgaaggcg gtatgaccaa cctggaacgt cagcagatcc tgactgaaga
     8941 gcagtatctg gacgcgctgg aagagttcgg tgacgaattc gacgcgaaga tgggtgcgga
     9001 agcaatccag gctctgctga agagcatgga tctggagcaa gagtgcgaac agctgcgtga
     9061 agagctgaac gaaaccaact ccgaaaccaa gcgtaaaaag ctgaccaagc gtatcaaact
     9121 gctggaagcg ttcgttcagt ctggtaacaa accagagtgg atgatcctga ccgttctgcc
     9181 ggtactgccg ccagatctgc gtccactggt tccgctggat ggtggtcgtt tcgcgacttc
     9241 tgacctgaac gatctgtatc gtcgcgtcat taaccgtaac aaccgtctga aacgtctgct
     9301 ggatctggct gcgccggaca tcatcgtacg taacgaaaaa cgtatgctgc aggaagcggt
     9361 agacgccctg ctggataacg gtcgtcgcgg tcgtgcgatc accggttcta acaagcgtcc
     9421 tctgaaatct ttggccgaca tgatcaaagg taaacagggt cgtttccgtc agaacctgct
     9481 cggtaagcgt gttgactact ccggtcgttc tgtaatcacc gtaggtccat acctgcgtct
     9541 gcatcagtgc ggtctgccga agaaaatggc gctggagctg ttcaaaccgt tcatctacgg
     9601 caagctggaa ctgcgtggtc ttgctaccac cattaaagct gcgaagaaaa tggttgagcg
     9661 cgaagaagct gtcgtttggg atatcctgga cgaagttatc cgcgaacacc cggtactgct
     9721 gaaccgtgca ccgactctgc accgtctggg tatccaggca tttgaaccgg tactgatcga
     9781 aggtaaagct atccagctgc acccgctggt ttgtgcggca tataacgccg acttcgatgg
     9841 tgaccagatg gctgttcacg taccgctgac gctggaagcc cagctggaag cgcgtgcgct
     9901 gatgatgtct accaacaaca tcctgtcccc ggcgaacggc gaaccaatca tcgttccgtc
     9961 tcaggacgtt gttctgggtc tgtactatat gacccgtgac tgtgttaacg ccaaaggcga
    10021 aggcatggtg ctgactggcc cgaaagaagc agaacgtctg tatcgctctg gtctggcttc
    10081 tctgcatgcg cgcgttaaag tgcgtatcac cgagtatgaa aaagatgcaa acggtgaatt
    10141 agtagcgaaa accagcctga aagacacgac tgttggccgt gccattctgt ggatgattgt
    10201 accgaaaggt ctgccttact ccatcgtcaa ccaggcgctg ggtaaaaaag caatctccaa
    10261 aatgctgaac acctgctacc gcattctcgg tctgaaaccg accgttattt ttgcggacca
    10321 gatcatgtac accggcttcg cctatgcagc gcgttctggt gcatctgttg gtatcgatga
    10381 catggtcatc ccggagaaga aacacgaaat catctccgag gcagaagcag aagttgctga
    10441 aattcaggag cagttccagt ctggtctggt aactgcgggc gaacgctaca acaaagttat
    10501 cgatatctgg gctgcggcga acgatcgtgt atccaaagcg atgatggata acctgcaaac
    10561 tgaaaccgtg attaaccgtg acggtcagga agagaagcag gtttccttca acagcatcta
    10621 catgatggcc gactccggtg cgcgtggttc tgcggcacag attcgtcagc ttgctggtat
    10681 gcgtggtctg atggcgaagc cggatggctc catcatcgaa acgccaatca ccgcgaactt
    10741 ccgtgaaggt ctgaacgtac tccagtactt catctccacc cacggtgctc gtaaaggtct
    10801 ggcggatacc gcactgaaaa ctgcgaactc cggttacctg actcgtcgtc tggttgacgt
    10861 ggcgcaggac ctggtggtta ccgaagacga ttgtggtacc catgaaggta tcatgatgac
    10921 tccggttatc gagggtggtg acgttaaaga gccgctgcgc gatcgcgtac tgggtcgtgt
    10981 aactgctgaa gacgttctga agccgggtac tgctgatatc ctcgttccgc gcaacacgct
    11041 gctgcacgaa cagtggtgtg acctgctgga agagaactct gtcgacgcgg taaaagtacg
    11101 ttctgttgta tcttgtgaca ccgactttgg tgtatgtgcg cactgctacg gtcgtgacct
    11161 ggcgcgtggc cacatcatca acaaaggtga agcaatcggt gttatcgcgg cacagtccat
    11221 cggtgaaccg ggtacacagc tgaccatgcg tacgttccac atcggtggtg cggcatctcg
    11281 tgcggctgct gaatccagca tccaggtgaa aaacaaaggt agcatcaagc tcagcaacgt
    11341 gaagtcggtt gtgaactcca gcggtaaact ggttatcact tcccgtaaca ccgaactgaa
    11401 actgatcgac gaattcggtc gtaccaaaga aagctacaaa gtgccttacg gtgcggtact
    11461 ggcgaaaggc gatggcgaac aggttgctgg cggcgaaacc gttgcaaact gggacccgca
    11521 caccatgccg gttatcaccg aagtaagcgg ttttgtacgc tttactgaca tgatcgacgg
    11581 ccagaccatt actcgtcaga ccgacgaact gaccggtctg tcttcgctgg tggttctgga
    11641 ttccgcagaa cgtaccgcag gtggtaaaga tctgcgtccg gcactgaaaa tcgttgatgc
    11701 tcagggtaac gacgttctga tcccaggtac cgatatgcct gcgcagtact tcctgccggg
    11761 taaagcgatt gttcagctgg aagatggcgt acagatcagc tctggtgaca ccctggcgcg
    11821 tattccgcag gaatccggcg gtaccaagga catcaccggt ggtctgccgc gcgttgcgga
    11881 cctgttcgaa gctcgtcgtc cgaaagagcc ggcaatcctg gctgaaatca gcggtatcgt
    11941 ttccttcggt aaagaaacca aaggtaaacg tcgtctggtt atcaccccgg tagacggtag
    12001 cgatccgtac gaagagatga ttccgaaatg gcgtcagctc aacgtgttcg aaggtgaacg
    12061 tgtagaacgt ggtgacgtaa tttccgacgg tccggaagcg ccgcacgaca ttctgcgtct
    12121 gcgtggtgtt catgctgtga ctcgttacat cgttaacgaa gtacaggacg tataccgtct
    12181 gcagggcgtt aagattaacg ataaacacat cgaagttatc gttcgtcaga tgctgcgtaa
    12241 agctaccatc gttaacgcgg gcagctccga cttcctggaa ggcgaacagg ttgaatactc
    12301 tcgcgtcaag atcgcaaacc gcgaactgga agcgaacggc aaagtgggtg caacttactc
    12361 ccgcgatctg ctgggtatca ccaaagcgtc tctggcaacc gagtccttca tctccgcggc
    12421 atcgttccag gagaccactc gcgtgctgac cgaagcagcc gttgcgggca aacgcgacga
    12481 actgcgcggc ctgaaagaga acgttatcgt gggtcgtctg atcccggcag gtaccggtta
    12541 cgcgtaccac caggatcgta tgcgtcgccg tgctgcgggt gaagctccgg ctgcaccgca
    12601 ggtgactgca gaagacgcat ctgccagcct ggcagaactg ctgaacgcag gtctgggcgg
    12661 ttctgataac gagtaatcgt taatccgcaa ataacgtaaa aacccgcttc ggcgggtttt
    12721 tttatgggtg ttattcagag ggcaaattct tcacggaaaa tattaagaaa ggcagggcga
    12781 aacgaggaag aagcttttcg ccctggacaa cattcctgct gacggcacta ccataaaaaa
    12841 acacaacgct ttcaacaacc ttgcgttttt catcttccgt agcggctcgg aataaattta
    12901 cgttcttaca ttccgtctca tagtctttgc gaggcgcgtc ccagatagct gtcccagtct
    12961 ttccataccg gctgcaaacc ctgagcggtt aacgcggcag caaccgcttc cggtctgcga
    13021 tcgtcgtgcg gtgagaactg ttccaactcg gggtgattat cggcatagcc acctggctgc
    13081 gttttcgaga aggcgctgac gttattgatc gccagcggaa taacgcgatc gcgaaaccac
    13141 ggtgattccc gcgtggagag tgacagttca atctccggtg caagcagtcg gaaggcgcag
    13201 atggtttgca ctaactggcg ttcatccata atcgacgcag gctcaatgcc gccagtacac
    13261 gggcgcaggc gcggaaagga gacagagtaa cggctttgcc agtaatgctg ttgcagccat
    13321 agcaaatgtt cggcaaccat atagcagtca acgcgccagt tgtcggaaag gccaattagc
    13381 gcgccgaggc ctatcttatc aatccccgca cgccccagcc gatccggcgt ttccagccgc
    13441 cagaagaagt cctgtttttt gcctttcaaa tgatggcggg tataagtcgc ctcgtgatat
    13501 gtctcctgat aaaccatcac cccatccaga ccaagttgct ttaactcggc gtattccgtc
    13561 tccgccagcg gttgcacttc catttgtagt gaagagaact gttcacgcag ggcggggaga
    13621 tgacgacgaa agtaatccat ccccactttc gcctgatgtt caccagtgac taatagcaga
    13681 tgttcaaagc ccatctcccg tatagcggcg ctttccctgg caatatccgc ttcatccagc
    13741 gttttgcgct tgatgcgatt actcatggaa aatccgcagt acgtgcagtc gttagcgcaa
    13801 agattggaaa gataaagcgg gacgtagaaa ctaactgtgt tgccaaatcg ctgacgggtc
    13861 agacgctgcg cccgttgggc cagtggttcc agatagccac tggcggctgg cgataacagc
    13921 gccatcatat cgtcgcgggt gagttgcgag gcatttagcg cccgttctac gtcagcagcc
    13981 gttttgccgt tgatacgcag gcggatgtcg tcccagtcca gttgtcgcca gcgatcgctg
    14041 aaggttttca tgccgatgcc tccagaaatc cagtcagcgg gctggtggca tgagcaaaat
    14101 gactgcggct gcccggtccg gactgacgtg ccagcaggcc tgcttctacc gccagacgaa
    14161 atgccttcgc catgttgacg ggatcgtccg ctacggcaat cgccgtattc actaacaccg
    14221 cgtcggcccc catttccagg gcctgcgcgg catggctggg aacgccgatg ccagcatcga
    14281 caaccaccgg aactgtggcc tgctggataa taatctccag catggcacgg gtttccagtc
    14341 cctgattcga gccaatcggc gcgccgagcg gcatcaccgc tgcacagccg acttcttcca
    14401 gacgtttaca caataccgga tcggccccgc agtaaggcag cacgacaaat ccctgttgta
    14461 ccagcatttc ggcggctttc agagtttcga tgggatcggg caacagccag cgggcgtcag
    14521 ggtgaatctc taattttaac cagtttgtgc ctaacgcttc acgtgccaga tgggcggcga
    14581 aaatggcttc ttccgctgtt ttcgccccgg aagtatttgg cagcagggtc acacccgccg
    14641 cgataagcgg ttcgaggata gcgtcgttgt gctggcgcaa gtcgacacgt ttcatcgcca
    14701 gtgtcaccag ctggctgccg gaagcgcgga tcgcctccac catcagttgt gaagaagcga
    14761 atttccctgt gccggtaaac agatgtgaat caaacgtttt gtccgcaata cgtaacatct
    14821 caaccccctg caataacctg aaaaagcagg atctggtcgc catcctgcac gatatgttgc
    14881 gtccactgct cacgcgggac gatttgctga ttaatcgcca gagccgcgcc cgcttgtcgt
    14941 tggtcgagtt gctccagtag ttcgtgaaca gtttgcccgg cggcgcactg catcggttga
    15001 tcgttaaaca ggatctgcat tgcttcctcc gcataccggg caaccactgg cgcggcgcaa
    15061 cgccaggctg cgccactggc tcgatttacc gtcgaacagt cggagttctc ccgcaggtgt
    15121 ctctataccg cttaataact taatggcttc cagtgcctgc aaagtgccca taaccccgac
    15181 caccgggcca accacgcccg ccgtgcggca gttgcgttct ggctcctggt tatctggcca
    15241 caggcagcgg taacacccct gctcccaggg cggcgtcagt accatcaact gaccgccaaa
    15301 tccgaccgcg ctggcggtga taagcggcgt gttgagtacc acgcaggcgg cattaatctc
    15361 ctggcgagtc gccatattgt cggtacagtc gagcaccaca tcggcccgtg caaccgcatc
    15421 ttttaacgcc tcacccgtta accgttgttg taatgctgtc agttgaatat cgggattcaa
    15481 ctgtgtcagt cgctgatggc tgacctgcga tttcgggcga tcgatatctt cagtggtaaa
    15541 gaggatttgt cgttgcagat tgcttaaatg cacatcgtcg tcatctgcca gtaccagcgt
    15601 cccgacgcca gcccccgcca ggtatagcgc agcaggtgta cccagcccgc caagaccaat
    15661 aatcagcacc tggctgtcga gcagtttttg ctgcccgtcc agagcgatat cgtcgagcag
    15721 aatttggcgg ctataacgca taaagtcacg gtcattcatc gccaactcct gcaatttcca
    15781 gcaactgtgc cgttgccaaa cgccagtctg cggcttgagt aatggcgctg acgacggcga
    15841 tactgccgac acccgttgct atcaccgcag gcgcgcgcgc cagactgata ccgccaatcg
    15901 ccacggtggg ataatccgcc agtcgctcaa catgccgtgc cagctgttcc agcccctgcg
    15961 gtgcagaagg catctgtttg gtttgcgtcg ggaacacatg tcccagcgcg atataagagg
    16021 ggcgtgctgc cagcgcgacg tcgatttcca tatcgtcatg tgtcgaaacg cccagccgca
    16081 ggcctgccgc gcggatggca ttgagatcgg tggcttgcaa atcttcctgc cccaaatgga
    16141 cgccatacgc ctgatgcttg atcgccagcc gccagtaatc gttgataaac aatcgcgcgt
    16201 tatagcggcg gcccagcgca attgccgcca cgacatcggc ttccacctct tcattgcgcc
    16261 gatctttgat gcgtagctgg agagtacgta cgcctgcatc caacagacgt tcgatccact
    16321 gtacgctgtc caccaccggg tacagtccta aacgaaaagg tacaggagga aaatcaggct
    16381 gatacatcac gcttcctcct tacgcaggta gatttctccg cctctggcgc ggaagttctc
    16441 cgacatatcc gccattccca cttcaatggt ttgcgcggcg gcgtaatcac gtacttcctg
    16501 gctgattttc atcgagcaga atttcggccc acacatggag caaaaatggg cgactttacc
    16561 tgactcttgc ggcagggttt catcgtgata agcgcgggcg gtaaacgggt cgagggccag
    16621 attaaactgg tcttcccagc gaaattcgaa gcgggctttc gacatggcgt tatcgcgaat
    16681 ttgcgcgcct ggatgccctt tcgccaggtc agcggcgtgg gcggcaatct tataggtgat
    16741 aagcccctgc ttaacatctt ctttattagg cagacccaga tgctcttttg gcgttacgta
    16801 acagagcatc gcgcagccaa accagccaat catcgccgca ccaatccccg acgtgaagtg
    16861 gtcatagccc ggcgcaatat cggtagttag cggccccaga gtgtaaaacg gcgcttcgtg
    16921 gcagtgctct aactcctcgg tcatattgcg gcggatcatc tgcatcggca cgtggcctgg
    16981 gccttcaatc atcacctgca cgtcatattc ccaggcaatt ttggtcagtt cgcccagcgt
    17041 atgcagctcg gcaaactgcg cttcatcgtt ggcgtcctga atagaaccgg ggcgcagacc
    17101 gtcgcccagc gacagcgaaa cgtcataagc ggcacagatt tcacaaattt cgcggaagtg
    17161 ttgatagagg aaattttcct gatgatggga gaggcaccat ttcgccataa tcgaaccgcc
    17221 gcgagagacg ataccggtca ggcgtttcgc ggtcatcggc acatagcgca gcagtacgcc
    17281 cgcatggata gtgaagtaat ccacaccttg ctcggcctgt tccagcagcg tgtcgcggaa
    17341 cgcttcccag gtaagatctt cggcgatccc gttaaccttc tccagcgcct ggtagatcgg
    17401 cactgtaccg atcggcaccg ggctgttacg caaaatccac tcgcgggttt cgtgaatata
    17461 gcgaccggtg gagagatcca tcaccgtatc cgctccccag cgcgtggacc ataccagctt
    17521 ttccacttct tcttcgatgg aagaggtgac cgccgagttg ccgatattgg cgttaacttt
    17581 taccaggaaa ttgcgaccaa taatcatcgg ctccgattcc ggatgattaa tgttggccgg
    17641 gataatcgca cgtccggcag caacttcatc acggacaaat tccgcagtga tattttccgg
    17701 cagatgtgcg ccaaagctca ttcccggatg ctggtggcgt aaaacctcgc tacggatgcg
    17761 ctcgcggccc atattctcgc ggatggcgat gaattccatt tccggcgtga tgatgccctg
    17821 gcgggcgtag tgcagttggg tgacacggcg tcctgttttg gcgcgttttg gcgttaataa
    17881 gccgctaaaa cgtagctcgt cgagaccatc atctgccagc cgcgttctgg tgtaatcgga
    17941 actgcgcacg gtaagttctt cggtatcgcc gcgcgcatcg atccacggct ggcgtagttt
    18001 tgccagccct tgctgcacgt taatggcgat ctgcggatca ccatacgggc cggaggtgtc
    18061 gtagaccgga atcgcttcgt tttcttcgta ctgcggctgt tctttgctac cgccaattag
    18121 cgtcgggcta agctggatct cacgcatcgg cacgcgcacg ccggggtgtg tgccagtgat
    18181 ataaatgcgt tttgagttgg gaaaggcggt gccttccagg gtgtcgataa aatgttgggc
    18241 ccgggcgcgt tgttcgcggc gggtcagttt tgttgcagac atagctcatt ccaaaaagtc
    18301 aaggacgtgg cttgtcagac gacggatgaa gcaagagacg atcgccgcaa aggcgatgcg
    18361 atagcagatt aactcttgtt cccttcgcag gtattagcct gatcaggttc cgcggatccc
    18421 gaataaacgg tctcagccag ttaaggcact ccgacaagaa attagccccc acaaacgggg
    18481 cgttgaatgt aaattacgcg ttaacagcgc agaactcaag caggatgttt gacgcgggcg
    18541 gcgtcaagca ccagcagaat cagcttatct tccagcacaa agcgcgcttc cagcgcctcg
    18601 ccgatgtcag ataaaacctg ttgaaactca aggtaattat catgatcgat ggcggtttcc
    18661 aggctggaat cgtagtaatc cataatctgt tgggtattgg cttcgagttg cggccaaatc
    18721 ttcgcggcgc gcgcgagttg cccgttgcct tccagcttat gaagaatgcg ctcataaata
    18781 ctgaaatgtc cggcagacaa gtaatcgacc aggctctgac aaaaatcatc aagggctttt
    18841 tcgtttagcc tcatgtacga ttctttgcca agcttaatgc caaccagatt gtagtaagcc
    18901 acgagcagat gcttacgtac atgtagccag cgatcaacca gtttgttact tcctctgacg
    18961 cgctccgtca ggttatcgag ctggttaagc atgattgact ccgcaagttt gtattcaaaa
    19021 actgctcagt gagaaatgta aaaaccatgt taaacatgcc agtgatgcaa aggtagtgca
    19081 agagctatgg atcgtataat tgaaaaatta gatcacggct ggtgggtcgt cagccatgaa
    19141 caaaaattat ggttgccgaa gggagaattg ccatatggcg aagcggcaaa tttcgatctt
    19201 gtgggtcagc gcgcactaca aatcggcgaa tggcaggggg aacctgtttg gttagtacaa
    19261 cagcagcggc gtcacgatat ggggtcggta cgtcaggtca ttgatctcga tgttgggctg
    19321 tttcaactgg ccggacgagg cgtacaactg gcggagtttt accgatcgca taaatactgt
    19381 ggttactgcg ggcatgaaat gtatccgagc aaaaccgaat gggcgatgct gtgcagccat
    19441 tgccgtgagc gttactaccc gcaaatcgcc ccctgcatta ttgttgccat ccgtcgcgat
    19501 gattcgatcc tcctcgccca gcatacccgc catcgtaacg gtgtccatac agtacttgcc
    19561 ggattcgtcg aagtgggcga aaccctcgag caggcagtcg cgcgggaagt gatggaagag
    19621 agcggaatta aagttaaaaa cttgcgttac gtgacttctc agccgtggcc gtttcctctg
    19681 tctttaatga ccgcgtttat ggcggaatat gacagcggcg acatcgtgat cgacccgaaa
    19741 gaattgctcg aggcgaactg gtatcgctat gacgatttgc cgttactccc gccgcccggc
    19801 accgtagcgc gccgtctgat agaagatact gtggcgatgt gtcgggcaga gtatgagtga
    19861 tgatacactg accgcctgac gcactaagga acagccaaaa tgaccgaact taaaaacgat
    19921 cgttatctgc gggcgctgct gcgccagccc gttgatgtca ctccagtatg gatgatgcgc
    19981 caggcgggtc gctatctacc ggaatataaa gccacgcgcg cccaggcggg cgattttatg
    20041 tcgctgtgca aaaacgccga gctggcgtgc gaagtgactt tgcaaccgct gcgtcgctac
    20101 ccgctggatg cggcgatcct cttttccgat atcctcaccg tgccggacgc gatggggtta
    20161 gggctctatt ttgaagccgg agaaggtccg cgttttacct cgccagtcac ctgcaaagcc
    20221 gacgtcgata aactgccaat tccggacccg gaagatgagc tgggttacgt gatgaacgcg
    20281 gtgcgtacca ttcgtcgcga actgaaaggc gaagtgccgc tgattggttt ttccggcagc
    20341 ccgtggacgc tggcgaccta catggtggaa ggcggcagca gcaaagcgtt caccgtgatc
    20401 aaaaaaatga tgtatgccga tccgcaggcg ctgcacgctc tactcgataa actggcgaaa
    20461 agcgtcactt tgtatctgaa tgcgcagatt aaagccggtg ctcaggcagt gatgattttc
    20521 gacacctggg gcggtgtgct taccgggcgc gattatcaac agttctcgct ctattacatg
    20581 cataaaattg ttgatggttt actgcgtgaa aacgacggtc gccgcgtgcc agtcacgctg
    20641 tttaccaaag gcggcggaca gtggctggaa gcgatggcag aaaccggttg cgatgcgttg
    20701 ggcctcgact ggacaacgga tatcgccgat gcgcgccgcc gtgtgggcaa taaagtcgcg
    20761 ttgcagggta atatggatcc gtcgatgctg tacgcgccgc ctgcccgcat tgaagaagaa
    20821 gtagcgacta tacttgcagg tttcggtcac ggcgaaggtc atgtctttaa ccttggtcac
    20881 ggcattcatc aggatgtgcc gccagaacat gctggcgtgt tcgtggaggc agtgcatcga
    20941 ctgtctgaac aatatcaccg ctaaggagtg attatggatc tcgcgtcatt acgcgctcaa
    21001 caaatcgaac tggcttcttc tgtgatccgc gaggatcgac tcgataaaga tccaccggat
    21061 ctgatcgccg gagccgatgt cgggtttgag cagggcggag aagtgacgcg agcggcgatg
    21121 gtgctgctga aatatccctc gcttgagctg gtggagtata aagttgcccg catcgccacc
    21181 accatgcctt acattccagg ttttctttcc ttccgcgaat atcctgcgct gctggcagcg
    21241 tgggagatgc tgtcgcaaaa gccggattta gtgtttgtcg atggtcatgg gatctcgcat
    21301 cctcgccgtc ttggcgtcgc cagccatttt ggtttgatgg tggatgtgcc aacgatcggc
    21361 gtggcgaaaa aacggctctg cggtaaattc gaaccgctct ccagcgaacc gggcgcgctg
    21421 gccccactga tggataaagg cgagcagctg gcctgggtct ggcgcagcaa agcgcgctgt
    21481 aacccgttgt ttatcgctac cggccatcgg gtcagcgtgg acagcgcgct ggcgtgggta
    21541 caacgctgca tgaaaggcta tcgtctgccg gagccaacgc gctgggcgga cgcggtggcc
    21601 tcggaacgtc cggcgttcgt gcgctataca gcaaatcagc cctaattcag gtaaactgcg
    21661 gataatttcc gtatttgaga actcaacatg ttacaaaacc caattcatct gcgtctggag
    21721 cgcctggaaa gctggcagca tgtcactttc atggcttgct tatgcgaacg catgtacccc
    21781 aattacgcca tgttctgcca gcaaaccggt tttggtgatg ggcaaattta ccgtcgtatt
    21841 ctcgatctca tctgggaaac gctgaccgtt aaagacgcaa aagtaaattt cgacagccaa
    21901 ctggagaaat ttgaagaagc gattccttca gccgacgatt tcgatctgta cggcgtttat
    21961 ccggcaatcg atgcctgcgt ggcgttaagt gaactggtcc attcgcgttt gagtggtgaa
    22021 acgctcgaac acgcggtgga agtgagtaag acctccatca cgaccgttgc gatgctggaa
    22081 atgactcagg ctggtcgcga aatgagcgat gaagagctta aagaaaaccc agctgtagag
    22141 caagaatggg acattcagtg ggaaatattc cgacttttag ctgagtgcga agaacgcgat
    22201 atcgagctga taaaaggcct tagggcagac ctgcgtgagg cgggtgagag caatattggt
    22261 ataatttttc agcaataaga ccagaaaacg tgatttaacg cctgatttgt cgtacctgga
    22321 gtcttccctt tcgccccccg tctggtctac atttgggggg cgaaaaaaag tggctatcgg
    22381 tgcgtgtatg caggagagtg ctattctggc atttccgtcg cactcgatgc ttagcaagcg
    22441 ataaacacat tgtaaggata acttatgaac aagactcaac tgattgatgt aattgcagag
    22501 aaagcagaac tgtccaaaac ccaggctaaa gctgctctgg agtccactct ggctgcaatt
    22561 actgagtctc tgaaagaagg cgatgctgta caactggttg gtttcggtac cttcaaagtg
    22621 aaccaccgcg ctgagcgtac tggccgcaac ccgcagaccg gtaaagaaat caaaattgcc
    22681 gcagctaacg taccggcatt tgtttctggc aaggcactga aagacgcagt taagtaagat
    22741 tgcgtggcag tgaacagttt taacgaaggg gtggtttcac cccttttgtc tttctggcgt
    22801 cgatcattga tgctggctgg cgctctgctt ctcactgcct gtagtcataa ctcttcactt
    22861 cctcccttta ccgccagtgg atttgctgaa gaccagggcg cggtacgcat ctggcgaaaa
    22921 gacagcggcg ataatgtgca tctgcttgcc gtgtttagcc cgtggcgcag tggcgatacc
    22981 acgacgcgag agtatcgctg gcagggcgat aacctcacgc tcatcaatat caatgtttac
    23041 agcaaaccgc cggtgaatat tcgcgcgcgt tttgacgatc gcggtgatct gagctttatg
    23101 caacgtgaat ccgatgggga aaagcagcag ctttctaacg accaaatcga tttataccgt
    23161 tatcgtgctg atcagatccg ccagattagc gatgccttac gtcaggggag agtcgtgctg
    23221 cgccaggggc gctggcatgc gatggaacag accgtgacca cctgcgaagg gcaaaccatt
    23281 aaacctgatt tagattcgca ggcgatagcg catatcgagc gccgccagag ccgctcttct
    23341 gttgatgtca gcgtggcatg gctggaagcg cccgaaggtt cgcaattact gttagtggca
    23401 aactctgatt tctgtcgctg gcaacccaac gagaaaacgt tctgatttac cagtggccca
    23461 tacccatatg accgccacca ccgcagccgc cgtagcccat gcccattccg gctccgcgcg
    23521 gaatacccgc ttcagccatc gcgatatctc gtttcacccg taactcatct aacgactgac
    23581 gcaaattctc catctctttg gcgaccgcgt taattttgct gctatccggt gggttcgcgg
    23641 ctaacagggc attgtattca taacgcttcg tcaccagttg ctgttgcagt gcgctgcttt
    23701 gagcgtaaaa gtcattatgg attttctgcc acgctgtctg ttgttcgctg gtcaaaggcg
    23761 cggcattttg ctgccacata ccgtgtccgc cgtgagcaaa tgcagatgtc gatcccatcg
    23821 ccattgctga aagcgccatc attaccaggg caattttcgt gttccgtttc atggttaatc
    23881 ctccagtggt tgtcttactt cgggtattgc atcttccgtg ccaacgatga aacgctgata
    23941 tgacgggtaa tctggcatga taaacgagta aaaatgactc gcctgctgcg ggtagcgagt
    24001 catttttact cattgaaact taagcctttg tgttacagcg cagggtaagc gctgataaaa
    24061 gatggcatga tttctgctgt cagaaaggga tgagcaggca aagaagaaga tgcgttttat
    24121 gcaacgttct aaagactcct tagctaaatg gttaagcgcg atcctccccg tggtcattgt
    24181 tgggctggta gggttgtttg cggtgacggt gattcgcgat tatgggcgcg agactgccgc
    24241 cgccagacaa acgctgctgg aaaaaggcag tgtacttatt cgcgctcttg aatccggctc
    24301 gcgcgtcggc atggggatgc gcatgcatca tgcgcagcag caggccttac tggaagaaat
    24361 ggccgggcag cctggagtac gttggtttgc ggtcacggat gaacaaggaa caatcgtgat
    24421 gcatagcaac tccggcatgg tgggaaaaca gctttattcc ccgcaggaaa tgcagcagtt
    24481 acatccggga gatgaagaag cgtggcggcg gatcgatagc gcagacggcg agcctgttct
    24541 ggaaatttat cgccagtttc aaccgatgtt tgctgctgga atgcaccgga tgcgccatat
    24601 gcagcagtat gccgcgacac cacaagcaat tttcatcgct ttcgacgcca gtaatattgt
    24661 gagtgccgaa gatcgtgagc agagaaacac cctgattatc ctcttcgccc tggcgacggt
    24721 cttgctggca agcgtattgt cattcttctg gtatcgccgc tatttgcgct cgcgccagct
    24781 tctacaagat gaaatgaagc gcaaagagaa gctggtggcg ctggggcatc ttgcggcagg
    24841 cgttgcccac gaaatccgta acccactttc ctcgattaaa ggactggcga aatactttgc
    24901 cgagcgcgcg cctgcagggg gagaagcgca tcaactggcg caggtgatgg cgaaagaggc
    24961 cgaccgttta aaccgcgtgg taagcgagct gctggaactg gttaagccaa cgcatctggc
    25021 tttgcaggcg gtggatctca acacgctgat taaccactca ttacagctgg taagccagga
    25081 tgcgaacagc cgggagatcc agttacgctt taccgccaac gacacattac cggaaattca
    25141 ggccgatccg gacaggctga ctcaggtcct gttgaatctc tatctcaatg ctattcaggc
    25201 gattggtcag catggcgtga ttagcgtgac ggccagcgaa agcggcgcgg gcgtgaaaat
    25261 cagcgttacc gacagcggta agggaattgc ggcagatcag cttgatgcca tcttcactcc
    25321 gtacttcacc actaaagccg aaggcaccgg actggggctg gcggtcgtgc ataatattgt
    25381 tgaacaacac ggtggtacaa ttcaggtcgc aagccaggag ggaaaaggcg caacgttcac
    25441 cctctggctt ccggtcacta ttacgcgtaa gggcccacaa ggatgacgca cgataatatc
    25501 gatattctgg tggtggatga tgacattagc cactgcacta ttttgcaggc tttactgcgc
    25561 ggctggggct ataacgtcgc gctggcgaac agcgggcgac aggcgctgga gcaggtgcgg
    25621 gaacaggttt ttgatcttgt gctttgcgat gtgcgaatgg cggagatgga cggcattgcc
    25681 acgctaaaag agatcaaagc gttaaatccg gcgattccgg tgctgattat gaccgcatac
    25741 tccagcgtcg agacggcggt agaagcgttg aaaaccgggg cgctggatta tctcatcaag
    25801 ccgctggatt tcgataacct gcaggcgacg ctggaaaagg cgctcgcgca tacgcacagt
    25861 attgacgctg aaacgcctgc ggtgtctgcc agccagttcg gtatggtcgg taaaagcccg
    25921 gcgatgcaac acctgctcag tgaaatcgcc ctcgtcgcgc catcggaagc cacggtgctg
    25981 atccacggcg attccggcac cggtaaagag ctggtcgcca gggcgattca cgccagtagc
    26041 gcacgtagcg aaaaaccgct ggtaacgctc aactgtgcgg cactcaacga atccttgctg
    26101 gaatctgaat tgttcggtca cgaaaaaggg gcgttcactg gagccgatag acgccgggaa
    26161 gggcgctttg ttgaggcgga cggcggcacg ctgtttctcg atgaaattgg cgatatctcg
    26221 ccgatgatgc aggtgcgtct gctgcgtgcg attcaggagc gcgaagttca gcgtgtcggt
    26281 agcaaccaga ctatctcggt tgatgtccgg ttgattgcgg cgacccatcg cgatcttgcc
    26341 gcagaggtga atgccgggcg ttttcgccag gatctctact atcgcctgaa tgtggtggcg
    26401 attgaagtac catcgctgcg gcaacggcgg gaagatatcc ctctgctggc tgtccatttt
    26461 cttcagcgct ttgccgagcg taatcgcaag gcggtaaaag gctttacgcc acaggcgatg
    26521 gatttgctga tccactacga ctggccggga aatattcgtg aactggaaaa cgcggtggaa
    26581 cgggcggtgg tgctgctgac cggggaatat atttccgaac gcgagctgcc gctggcgatt
    26641 gccggtacgc cgatcccact ggggcaaagt caggatattc agccgctggt ggaagtcgaa
    26701 aaagaagtga ttctggcggc actggagaaa acgggcggca acaaaaccga agccgcccgt
    26761 cagttaggga tcacgcgcaa aacgctattg gcaaaactgt cgcgttagtt ctgctcgcgt
    26821 tcgatagcgc gccagccgat atctttccgg cagaagcagt cgtcccagtg aatatcggtc
    26881 attaaggcat aagcgcgttt ctgcgcttct gccacggtat gacccagcgc ggtgacgcac
    26941 agtacgcgcc cgccgctggt cactacctgc tcgttatccg ccagtttcgt gcccgcgtgg
    27001 aacactttgc cgcctgccac ttcttccagc ggcaggccgt ggatcacatc gccggtgcgg
    27061 taatcgcccg gatatccacc cgcagccatc accacgccga gagaagcacg ctcatcccac
    27121 tctgacgttt tctcgtccag tttgccttca caggccgcca ggcagagctc aaccagatcg
    27181 gacttcatgc gcagcataat cggctgggtt tctggatcgc caaagcggca gttaaattcg
    27241 ataaccttcg gattgccctg tttgtcgatc atcaggcccg cgtagagaaa accggtgtag
    27301 gtgttgcctt ccgccgccat gcctttcacg gttggccaga tgatacgttc catggtgcgc
    27361 tgatgaactt catcggttac taccggcgcg ggggagtaag cgcccatccc gcccgtgttt
    27421 ggtccggtat ctttatcgcc tacgcgtttg tgatcctggc tggtggccat cggcagcaca
    27481 tgctcgccgt ctaccatcac gataaagctc gcttcttcgc cgtcgaggaa ctcttcgata
    27541 acgatgcgat gacccgcgtc gccaaaagcg ttgcccgcca gcatatcgtg aacagccgct
    27601 tcggcttctt ccagcgtcat cgccacgata acgcctttcc cggctgccag accgtccgct
    27661 ttaatgacga tcggcgcacc tttctcacgc agatacgcca gcgcaggttc tacctcggtg
    27721 aagttctggt attccgctgt agggatctta tggcgggcca ggaaatcttt ggtaaacgct
    27781 ttcgaacctt ccagttgcgc ggcacctgcg gttgggccga agattttcat cccggcggcg
    27841 cggaaggtat cgaccacgcc tttcaccagc ggcgcttccg ggccgacgat ggtcagatca
    27901 atcttttcgt tttgtgcgaa atccaacagc gccgggatat cggtcacgcc gatagcaacg
    27961 ttttgcagcg cgggttccag cgcagtgcct gcattacccg gagcaacaaa aacagtttca
    28021 accagcggcg actgcgccgc tttccaggcc agcgcgtgct cgcgcccgcc gttaccaatc
    28081 actaatactt tcatctattg ctccattaat ggcggaagtg gcgcatgtcg gtgaagagca
    28141 tcgcaatacc gtgctcgtcg gcggcggcaa tcacttcgtc atcacggata gaaccgccag
    28201 gctggattac gcaggtcacg cctgcagcgg cggcggcatc aataccgtcg cggaacggga
    28261 agaacgcgtc agaagccatc gaggaacctt tcacttccag gccttcatcg gcagctttaa
    28321 taccggcgat tttcgcggag tacacgcggc tcatctggcc cgcgccaatc ccgatggtca
    28381 tattgttttt ggcatagacg atagcgttgg atttcacgaa cttcgccact ttccagcaga
    28441 acagcgcatc acgcagttcc tgttcggtcg gctgacgttg ggtgacgacg cgcagttctt
    28501 ccgcaccgac catgcccagg tcacgatcct gaaccagcag accgccgttc acgcgtttga
    28561 aatcaagacc cggaacacgc tcgccccact gaccgcaggt cagaacgcgt acgttctgct
    28621 tggcggcggt gattttcagg gcttcttcgc tggcggaagg cgcaataatc acttcgacaa
    28681 actgacgaga aatgatggcc tgtgcggttt ccgcatccag ctcgcggtta aaggcaatga
    28741 tgccgccgaa tgcggaggtt gggtcggttt tgtacgcgcg atcgtaagca tcaagaatgg
    28801 aattgccgat agccacgccg caagggttgg cgtgcttcac aatcacacat gccggctcgg
    28861 cgaactcttt cacgcactcc agcgccgcat cggtatcggc gatgttgtta taagagaggg
    28921 ctttaccctg aacctgggtt gcggtagcaa cggaggcttc tttcacattc tcttctatat
    28981 agaaggcagc ctgctggtgg ctgttctcgc cgtaacgcat atcctgcttc ttaatgaagt
    29041 tcaggttcag cgtgcgtggg aagcgaccgg cggcttcttt gctttcaccg tggtaagccg
    29101 gaaccatgct gccgaagtag ttggcaatca tgctgtcgta ggcggcagtg tgttcgaagg
    29161 ctttgatggc gaggtcgaaa cgggttgcaa gcgtcagcga tccttcgttg tcatccatct
    29221 ctttaataat ggcgtcgtag tcgctgctct tcaccacgat tgcgacatct ttatggttct
    29281 tagcggcgga gcgcaccatg gtcgggccgc cgatatcgat gttctcaacc gcatcttcca
    29341 gcgagcagcc ttcacgagca acagtctggg cgaacggata caggttaaca accaccatat
    29401 cgataggctg gatctgatgt tcttccataa tggcatcgtc ctggccgcga cggcccagaa
    29461 tgccaccatg tactttcgga tgcagggtct tcacgcgtcc atccatcatc tccgggaaac
    29521 cggtgtaatc ggaaacttcg gttaccggca gacctttttc tgctaacaga cgggcagtgc
    29581 cccctgtaga cagcagctcc acaccgcgtg cggaaagtgc ctgggcgaat tcgacgatac
    29641 cggctttgtc agaaacactg agcagagcgc ggcggactgg acgacgttgt tgcatggtaa
    29701 atcccctgga tttgactatt acagagagcg ttagctgaat ttttcgcgaa aaactcagct
    29761 aacgccccta acggggcatc cttatttttc gcccgcattg taacgaaaac gtttgcgcaa
    29821 cgctcgcgaa tttttctctt tcaatggtga tcacaatttt gacagtggtt accgtgggaa
    29881 aaatacagaa attacattga tgattgtgga taactctgtg cgtaaaaagg tataaagcgg
    29941 ggttttgctg gggaatgcag cagtcagtca tttttctgca atttttctat tgcggcctgc
    30001 ggagaactcc ctataatgcg cctccatcga cacggcggat gtgaatcact tcacacaaac
    30061 agccggttcg gttgaagaga aaaatcctga aattcagggt tgactctgaa agaggaaagc
    30121 gtaatatacg ccacctcgcg acagtgcgct aaag
//
LOCUS       DAZPDU010000036        29548 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZPDU010000036 DAZPDU010000000
VERSION     DAZPDU010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29548
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.036"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..689
                     /gene="dgt"
                     /locus_tag="ACHURS_001465"
     CDS             <1..689
                     /gene="dgt"
                     /locus_tag="ACHURS_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dGTP triphosphohydrolase"
                     /protein_id="HFR1237238.1"
                     /translation="EDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRS
                     NSLSRSTEDQFFMYLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLL
                     KLYKNVAVKHVFSHPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRF
                     PIESRLFHKLSTRHRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWD
                     EYRRLMAVEQ"
     gene            819..2243
                     /gene="degP"
                     /locus_tag="ACHURS_001466"
     CDS             819..2243
                     /gene="degP"
                     /locus_tag="ACHURS_001466"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFR1237239.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            2398..3555
                     /gene="cdaR"
                     /locus_tag="ACHURS_001467"
     CDS             2398..3555
                     /gene="cdaR"
                     /locus_tag="ACHURS_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFR1237240.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(3644..4030)
                     /locus_tag="ACHURS_001468"
     CDS             complement(3644..4030)
                     /locus_tag="ACHURS_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFR1237241.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(4192..5004)
                     /gene="yaeI"
                     /locus_tag="ACHURS_001469"
     CDS             complement(4192..5004)
                     /gene="yaeI"
                     /locus_tag="ACHURS_001469"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HFR1237242.1"
                     /translation="MISRRRLLQATAATIATSSGFGYMHYWELGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSVGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLMLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(5059..5883)
                     /gene="dapD"
                     /locus_tag="ACHURS_001470"
     CDS             complement(5059..5883)
                     /gene="dapD"
                     /locus_tag="ACHURS_001470"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFR1237243.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(5914..8586)
                     /gene="glnD"
                     /locus_tag="ACHURS_001471"
     CDS             complement(5914..8586)
                     /gene="glnD"
                     /locus_tag="ACHURS_001471"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFR1237244.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLHYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(8648..9442)
                     /gene="map"
                     /locus_tag="ACHURS_001472"
     CDS             complement(8648..9442)
                     /gene="map"
                     /locus_tag="ACHURS_001472"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFR1237245.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            9810..10535
                     /gene="rpsB"
                     /locus_tag="ACHURS_001473"
     CDS             9810..10535
                     /gene="rpsB"
                     /locus_tag="ACHURS_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFR1237246.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            10793..11644
                     /gene="tsf"
                     /locus_tag="ACHURS_001474"
     CDS             10793..11644
                     /gene="tsf"
                     /locus_tag="ACHURS_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFR1237247.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            11791..12516
                     /gene="pyrH"
                     /locus_tag="ACHURS_001475"
     CDS             11791..12516
                     /gene="pyrH"
                     /locus_tag="ACHURS_001475"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFR1237248.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            12808..13365
                     /gene="frr"
                     /locus_tag="ACHURS_001476"
     CDS             12808..13365
                     /gene="frr"
                     /locus_tag="ACHURS_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFR1237249.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            13457..14653
                     /gene="ispC"
                     /locus_tag="ACHURS_001477"
     CDS             13457..14653
                     /gene="ispC"
                     /locus_tag="ACHURS_001477"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFR1237250.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            14842..15600
                     /gene="ispU"
                     /locus_tag="ACHURS_001478"
                     /gene_synonym="uppS"
     CDS             14842..15600
                     /gene="ispU"
                     /locus_tag="ACHURS_001478"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFR1237251.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGRIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            15613..16470
                     /gene="cdsA"
                     /locus_tag="ACHURS_001479"
     CDS             15613..16470
                     /gene="cdsA"
                     /locus_tag="ACHURS_001479"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFR1237252.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            16482..17834
                     /gene="rseP"
                     /locus_tag="ACHURS_001480"
     CDS             16482..17834
                     /gene="rseP"
                     /locus_tag="ACHURS_001480"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFR1237253.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVMALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            17864..20296
                     /gene="bamA"
                     /locus_tag="ACHURS_001481"
     CDS             17864..20296
                     /gene="bamA"
                     /locus_tag="ACHURS_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFR1237254.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            20418..20903
                     /gene="skp"
                     /locus_tag="ACHURS_001482"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             20418..20903
                     /gene="skp"
                     /locus_tag="ACHURS_001482"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFR1237255.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            20907..21932
                     /gene="lpxD"
                     /locus_tag="ACHURS_001483"
     CDS             20907..21932
                     /gene="lpxD"
                     /locus_tag="ACHURS_001483"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFR1237256.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            22037..22492
                     /gene="fabZ"
                     /locus_tag="ACHURS_001484"
     CDS             22037..22492
                     /gene="fabZ"
                     /locus_tag="ACHURS_001484"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFR1237257.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            22496..23284
                     /gene="lpxA"
                     /locus_tag="ACHURS_001485"
     CDS             22496..23284
                     /gene="lpxA"
                     /locus_tag="ACHURS_001485"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFR1237258.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            23284..24432
                     /gene="lpxB"
                     /locus_tag="ACHURS_001486"
     CDS             23284..24432
                     /gene="lpxB"
                     /locus_tag="ACHURS_001486"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFR1237259.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKEHVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            24429..25025
                     /gene="rnhB"
                     /locus_tag="ACHURS_001487"
     CDS             24429..25025
                     /gene="rnhB"
                     /locus_tag="ACHURS_001487"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFR1237260.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            25062..28544
                     /gene="dnaE"
                     /locus_tag="ACHURS_001488"
     CDS             25062..28544
                     /gene="dnaE"
                     /locus_tag="ACHURS_001488"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFR1237261.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEAHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            28557..29516
                     /gene="accA"
                     /locus_tag="ACHURS_001489"
     CDS             28557..29516
                     /gene="accA"
                     /locus_tag="ACHURS_001489"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFR1237262.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAVSLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
BASE COUNT         7159 a         7121 c         8160 g         7108 t
ORIGIN      
        1 ttgaagatgc ggtagagaaa agaatattta ccgttgagca gctttatcat catttgcacg
       61 aggcgtgggg tcagcatgag aaaggttcgc tcttttcgct agtggttgaa aatgcctggg
      121 aaaaatcacg ctcaaatagt ttaagccgca gtacggaaga tcagtttttt atgtatttac
      181 gggtaaacac cctaaataaa ctggtaccct acgcggcaca acgatttatt gataatctgc
      241 ctgcgatttt cgccggaacg tttaatcatg cattattgga agatgccagc gaatgcagcg
      301 atcttcttaa gctatataaa aatgtcgctg taaaacatgt gtttagccat ccggatgtcg
      361 agcagcttga attgcagggc tatcgggtca ttagcggatt attagagatt tatcgtcctt
      421 tattaagcct gtcgttatca gactttactg aactggtaga aaaagaacgg gtgaaacgtt
      481 tccctattga gtcgcgctta ttccacaaac tctcgacgcg ccatcggctg gcctatgtcg
      541 aggctgtcag taaattaccg tcagattctc ctgagtttcc gctatgggaa tattattacc
      601 gttgccgcct actgcaggat tatatcagcg gtatgaccga cctctatgcg tgggatgaat
      661 accgacgtct gatggccgta gaacaataac caggcttttg taaagacgaa caataaattt
      721 ttaccttttg cagaaacttt agttcggaac ttcgcgctat aaaacgaatc tgaagaacac
      781 agcaattttg cgttatctgt taatcgagac tgaaatacat gaaaaaaacc acattagcac
      841 tgagtgcact ggctctgagt ttaggtttgg cgttatctcc gctctctgca acggcggctg
      901 agacttcttc agcaacgaca gcccagcaga tgccaagcct tgcaccgatg ctcgaaaagg
      961 tgatgccttc agtggtcagc attaacgtag aaggtagcac aaccgttaat acgccgcgta
     1021 tgccgcgtaa tttccagcag ttcttcggtg atgattctcc gttctgccag gaaggttcac
     1081 cgtttcagag ctctccgttc tgccaggggg gccagggcgg taatggcggc ggccagcaac
     1141 agaaattcat ggcgctgggt tccggcgtca tcattgatgc cgataaaggc tatgtcgtca
     1201 ccaacaacca tgttgttgat aacgcgacgg tgattaaagt ccagctgagc gatggtcgta
     1261 agttcgacgc gaagatggtt ggcaaagatc cgcgctctga tatcgcgctg atccaaatcc
     1321 agaacccgaa aaacctgacc gcaattaaga tggcggattc tgatgcactg cgcgtgggtg
     1381 attacaccgt agcgattggt aacccgtttg gtctgggcga gacggtaact tccgggattg
     1441 tctctgcgct ggggcgtagc ggcctgaatg ctgaaaacta cgaaaacttc atccagaccg
     1501 atgcagcgat caaccgtggt aactccggtg gtgcgctggt taacctgaac ggcgaactga
     1561 tcggtatcaa caccgcgatc ctcgcaccgg acggcggcaa catcggtatc ggttttgcta
     1621 tcccgagtaa catggtgaaa aacctgacct cgcagatggt ggaatacggc caggtgaaac
     1681 gcggtgagct ggggattatg gggactgagc tgaactccga actggccaaa gcgatgaaag
     1741 ttgacgccca gcgcggtgct ttcgtaagcc aggttctgcc taattcctcc gctgcaaaag
     1801 cgggcattaa agcgggtgat gtgatcacct cactgaacgg taagccgatc agcagctttg
     1861 ccgcactgcg tgctcaggtg ggtactatgc cggtgggcag caaactgacc ctgggcttac
     1921 tgcgcgacgg taagcaggtt aacgtgaacc tggaactgca gcagagcagc cagaatcagg
     1981 ttgattccag ctccatcttc aacggcattg aaggcgctga gatgagcaac aaaggcaaag
     2041 atcagggcgt ggtagtgaac aacgtgaaaa cgggcactcc ggctgcgcag atcggcctga
     2101 agaaaggtga tgtgattatt ggcgcgaacc agcaggcagt gaaaaacatc gctgaactgc
     2161 gtaaagtgct cgacagcaaa ccgtctgtgc tggcactgaa cattcagcgc ggcgacagca
     2221 ccatctacct gttaatgcag taatctccct caaccccttc ctgaatacgg gaaggggttc
     2281 tccttacaat ctgtgaactt cgccacaact ccatacatct tcatcatcct ttgggcattt
     2341 gcacaatgcc gtacgttacg tacttcctta tgctaagccg tgcataacgg aggacttatg
     2401 gctggctggc atcttgatac caaaatggcg caggatatcg tggcacgtac catgcgcatc
     2461 atcgatacca atatcaacgt aatggatgcc cgcgggcgaa ttatcggcag cggcgatcgt
     2521 gagcgtattg gtgaattgca cgaaggtgca ttgctggtac tttcacaggg acgagtcgtc
     2581 gatatcgatg acgcggtagc acgtcatctg cacggtgtgc ggcaggggat taatctaccg
     2641 ttacggctgg aaggtgaaat tgtcggcgta attggcctga caggtgaacc agagaatctg
     2701 cgtaaatatg gcgaactggt ctgcatgacg gctgaaatga tgctggaaca gtcgcggttg
     2761 atgcacctgt tggcgcagga tagccgtttg cgggaagaac tggtgatgaa cctgattcag
     2821 gctgaggaga atactcccgc acttactgaa tgggcgcaac ggctggggat tgatctcaat
     2881 caaccgcgag tggtggctat tgttgaggtc gacagcggtc agcttggcgt ggacagcgca
     2941 atggcggagt tacaacaact gcaaaatgcg ctgactacgc ccgagcgtaa taatctggtg
     3001 gcaattgttt cgctgaccga aatggtggtg ttgaaaccgg cgttgaactc ttttgggcgc
     3061 tgggatgcag aagatcatcg taagcgagtt gaacaactga ttacccgcat gaaagagtac
     3121 ggccagctgc gttttcgcgt ttcactgggc aactatttta ccggtcctgg cagtattgcc
     3181 cgatcctatc gtacggcgaa aacgacgatg gtggtgggta aacagcggat gccagaaagt
     3241 cgctgctatt tttatcagga tctgatgtta cctgtgttac tcgacagttt gcgtggcgac
     3301 tggcaggcca acgaactggc gcgaccgctg gcgcggctga aagcgatgga caataatggc
     3361 ttgctgcgac gaacgctggc ggcgtggttt cgccacaatg tgcaaccgct ggctacgtca
     3421 aaggcgctgt ttattcatcg taatactctg gagtatcggc ttaatcgtat atcggaactg
     3481 accgggcttg atttgggtaa ttttgatgac aggttgctgc tgtatgtggc gttacaactg
     3541 gatgaagagc ggtaggttat gcgttaaggt cgaggcggcg ctggctcatt cccctcagcc
     3601 cgaagggtag aggggaatgt ccgagttaac cgtcagttcg gcattattta cgcgttagtt
     3661 tttccagatc ggcttcaatc tcgctgatct tattggttac gactgactcc aggtgacgta
     3721 agtcgtcgag gatcttacgc ttaagatcaa cttcgctgcg gtcacgctgg cagatttgat
     3781 caagctcatc aatgatatac cgtagattcg ggctgatttc ctggacttct ttataaccct
     3841 gacccacacc atcagcgacg accgttttac gctgacgcgg atatttaaac ttcacgctct
     3901 tggcgaaaaa ctcgcctttg tctttctgga aatagatttt cagaatatcg ttgttggctt
     3961 cctgccggag gctgtaacga tcaatttctt caggattggt aatacccaga cttttcagat
     4021 tgtcgtacat agcgttacct caaaatgagt cagtaaattg tgcttatttt agcatttggc
     4081 atggcccgcc cggctcgact gtgatcgcag aaagcctttt tcttccagac ttctatcatg
     4141 gcgtaaaaat caaaaaatta cctgctttat tgtggtgata aaattcacgt tctacaccag
     4201 ttccagcatc gttacttccg ggcggcagtt cagacgcaaa ccatacaaac tgcccacgcc
     4261 acgggttgtg tagatgtgtc gttcgccaaa ggcatttaat ccggcgacgt aacgtttatc
     4321 ttcgacaggg gcaaaaggtt caccaaccaa cggtacgcgc agttgcccgc cgtgagtatg
     4381 gccgcacagc atcagatccc agggttcatc acgcatgact tctttgctgt cgggattatg
     4441 tgccagcatc agtcttggca gatttgcttc gctggcggga ggtggtttgc attgtccggc
     4501 ccataagtca ccggtgccta ccagttcgaa ttgcctgttc ggcgtggcga tcaccgtagc
     4561 ctggttaaac aacaccgtga tgcccactga tttcaacgtc tcgccaatta agtgattttt
     4621 ttctgtacca acagggcgat cgtggttgcc gaagcaggca aacgtcggcg cacattcggc
     4681 aaggggggag aggacatcac taaacgccga aaaattcagc ggcatatcaa ataatacgta
     4741 atcgccgccc agtaatatca aatcgggctt ttgttctatg ccaagagcaa tcgcgtcaga
     4801 aatcaggctt aaaggaacaa aacgagagta atggagatcg gccagaaaaa gaattttgaa
     4861 tggtgctgcg ttgtctttaa aaaaggcgag gcggtgacgg attaattcaa accagcctag
     4921 ctcccagtaa tgcatataac cgaaacctga gctcgtggct atcgtcgcgg cagtagcctg
     4981 caacaatcgg cggcgtgaaa tcatcgctca tccctgcaaa aaaaatcggg cagcgtcgtg
     5041 ctgcccgtgt gcatactttt agtcgatggt acgcagcagt tcgttaatgc cgactttgcc
     5101 gcgagttttc gcgtcaactt tcttaacgat aaccgcacag tagaggctgt atttgccatc
     5161 ttttgacggc agattacctg aaacaaccac agaccccgcc ggaacgcgac cgtagtggat
     5221 ttcgccggtt tcacggtcgt aaatacgggt gctctgacca atgtatacgc ccatggaaat
     5281 gacggaacct tcttcgacaa tcaccccttc aaccacttca gagcgtgcgc caatgaagca
     5341 gttatcttca atgatggttg ggttagcctg cagcggttcc agcacgccgc cgatgcccac
     5401 gccaccggaa aggtggacgt ttttaccaat ctgcgcacaa gaaccgacgg tcgcccaggt
     5461 atcaaccatg gtgccttcat caacatatgc gccgatgttg acgtaagacg gcatcagcac
     5521 ggtgttacgg gcaataaacg caccctgacg taccgccgct ggtggcacaa cgcggaagcc
     5581 ttctttctgg aaacgtgctt cgtcgtagtc ggcgaatttc atcggcactt tgtcgaagta
     5641 gcggctttct gccccttcga tcacctgatt atcattaata cggaaagaga gcagcaccgc
     5701 ttttttcaac cactgatgcg tcacccactg accgtcaatt ttttccgcta cacgcagtgc
     5761 accggaatcc agcagggcga tcacctgatt taccgcttcg cgggtaacgg tgtctgcgtt
     5821 ggctggcgtg atctcggcac ggcgttcaaa agcggtttca ataatgttct gtaactgctg
     5881 cattgttaaa ctcttttcat atcagtaaac acatcaccct ttatcgtttg gattgagggc
     5941 ctctgtcaac cgctgatgca cttcctgctg caactcgtta ttaagtgcac gccggtcagc
     6001 ggtggcaatt atgaataaat cttctactcg ctcgccaatg gttgtaattc gggcaccatg
     6061 aagcgaaatt cccagatcgg caaaaatttt cccgactctc gccagcagtc ctggctggtc
     6121 gagcgcgata agctcaagga acgatttgcg gtcggtatgg gttggcaaaa acgtcacttc
     6181 ggtttcaaca gtaaagtgac gtagtttagc cggttgacga cgcggctgtg gtggctgcca
     6241 gctactttgt gtcagtacct gctccagacc aaaccgaata acctcatgac gatctgcgga
     6301 tagcgggcta ccatcgggtt ccagcacgat gaaggtatcc atcgccatac cgtcgcgagt
     6361 ggtgaaaatt tgtgcgtcgt gaacacttaa attgcggcgg tctaattcgg cacagacggc
     6421 ggcaaacaga taggggcggt ccgggctcca gataaaaatc tcggtgcctc cacgcgtagc
     6481 ctgcgggcta agcaatacca gcggtttgct taaatcatgc tgtaataaat ggcgggcatg
     6541 ccaggccagt tgatttgggc tatggcggac aaaatagtta gcacgacagc gtgaccaaat
     6601 ttggtgcagc gcctcttcgt cgatgttatc catgcgcagt agtgccagtg cctggagttg
     6661 gtgatggcga acccgttcgc gcatatccgg cgtgttttgc attccgcgtc gtagctgctt
     6721 ttcggtggca aaatagagtt cgcgcaacag actttgcttc cagctattcc acagcgtttc
     6781 gttggtggcg caaatgtcag ccactgtcag gcataccaga tagtgcagac gattttccgt
     6841 ttgaacttct tcggcaaact gcttgatgac ttcagggtcc tgaatatcgc ggcgttgtgc
     6901 tgtcaccgac atcagcaagt gctggcgaac cagccaggca accagctgtg tttcgcgtga
     6961 gttcagcccg tggagttctg caaaatgcac tacatcctga gcaccgagaa tggagtggtc
     7021 gccgccgcgt cctttggcga tatcgtgaaa cagcgcggcg atgaaaatca gctcagttga
     7081 cggcaggcgc ggccagacgt ccacacacaa cggatggcgc tggcgcgttt cttcactggc
     7141 aaaactctcc agtttcagca tcacgcggat agtatgttca tccaccgtgt aggcgtggaa
     7201 cagatcaaac tgcatctgcc cgacgatatg cgaccattgc ggcatatacg cgccgagcac
     7261 gctatggcga tgcattggca atagcccgcg ccgcaccgct ccggggtgac gcagaatgct
     7321 caaaaacagt tttcgtgctt ccggaatatt acacagcggt tgttgcagat ggcgacgggc
     7381 atggcgtaac tggcgcagcg tggtggagta aatgccggtg atcgcactgt tgcgcaccat
     7441 ggtgtagaac atacgcaaga tggcttccgg ctggcgcata aatagtgttt catcacgcag
     7501 gtcgattagc gtaccgcgta gctgaaactc atcgtcgatt ggacgtggtt tttcgtcggc
     7561 gggaagggcg aggatggctt catcgaacag ttgcagcagc atctggttga gttcactgac
     7621 gcggcgtgta acgcggaagt aatccttcat catccgctcg accggttcgt tgccttcacc
     7681 actgtaattc agacgctgag cgacgctaag ttggcgatcg aataacaggc gattatcgta
     7741 acggctgacg accagatgca gggcaaagcg aatacgccac agtatatgca gacattcgtt
     7801 taattccgcc cgctccgctg aggttaagaa gccaaaccca accatttcat ccagcgatgt
     7861 tgcgccaaaa tgacggcggg ccacccattg cagagtgtgg atatcgcgca agccgccagg
     7921 gctacttttg atgtctggtt caagattgta gctggtgccg tggtaacgct gatggcgctg
     7981 gttctgttct tcaactttcg ccgcgtagaa cttgtcggaa ggccagaatc cttcgctaaa
     8041 aatatgtttt tgcagttcga ggaagagcgc aacatcgcca attaataagc gggattcgat
     8101 taaattggtg gcgacggtta aatccgataa cccttccagc atgcactctt caagcgtgcg
     8161 cacactatga ccgacttcca gctttacatc ccagagcagc gttaacagct cgcccacttt
     8221 ttgcgcctga tcgtccggga gctttttacg gcttaaaatc agtaaatcga tgtcagaaag
     8281 tggatgcagc tcgccacgac cgtagccacc gacggcgacc aatgccaggt cggcaatctg
     8341 gctgaatccc gcttcaatcc ataatcgttg caggagctgg tcgataaact cggtgcgcgc
     8401 ctcaatcaac tgttctgcag agatcccatt gtcaaaggca tcacccagcc aacgctggaa
     8461 agtatcgata tgggctttta tcccaccgac ggttaattca tcacggggcc agacgcatgg
     8521 attttgcggt tgaccgggca gggtggggag agcggtgttt gcgtactgtt ctggaagggt
     8581 attcattgtg cgccacccat aagattaaat tatcgcatta aaaaagccgg cattcgccgg
     8641 cttcatctta ttcgtcgtgc gagattatcg ccgggatggt gtcatccttg cgtagcgtca
     8701 gaatttcgca gccgttatca gtcaccacaa tagtatgctc atattgtgca gacaagctgc
     8761 gatctttggt ttttaccgtc cagccatctt tcatggtgcg gatctctttt ttacccgcgt
     8821 tgaccattgg ctcgatggtg aacgtcatcc caggtttcag tacgacgttg gtttcacggg
     8881 agtcatagtg cagcacctgc ggttcttcat ggaagccgcg accaataccg tgtccgcaat
     8941 attcacgaac gacggagaag ccttctgctt cgacaaactt ctgaatcgcc gcaccgattt
     9001 cgcgcaggtt aatgcctggt ttgaccatgc gcagcgccag gtacaggctt tcttgcgtga
     9061 tgcggcacag acgttcgccc atgatggtcg gcttaccgac gataaacatt ttcgaggtat
     9121 cgccgtggaa accatctttg attacggtga catcaatgtt aacgatatcg ccatctttca
     9181 gcagcttcgc atcgtccggg ataccgtggc acaccacttc attaatagag atgcaaacgg
     9241 atttcggata gccgtgatag ccgaggcagg cagaaaccgc gtgttgttca ttaacaatgt
     9301 aatcattaca gatgcgatcc agctcgccgg tgctgacgcc cggtttaaca tacggttcga
     9361 tcatctccag cacttcggca gccagtcggc cagcgacgcg cattttttcg atatcttctg
     9421 gggtcttgat tgagatagcc attaattctg tccatcagcg tcggtgatac cgacaatata
     9481 tatgtaagtg ccgtcaatgg tatcacaccc gggcaaattg agaatcattc tgaatttcgc
     9541 caaacgtgcc actgaacgtt ttctataata gaaaattcaa cgtctgatgc tgtacacagc
     9601 gccaacaatt attggtgtcc atgacgtatt tgtggtataa agcgcgccgg acttccgatc
     9661 catttcgtat acacagactg gacggaagcg acaatctcac tttgtgtaac aacacacacg
     9721 tatcggcaca tattccgggg tgccctttgg ggtcggtaat atgggatacg tggaggcata
     9781 accccaactt ttatatagag gttttaatca tggcaactgt ttccatgcgc gacatgctca
     9841 aggctggtgt tcacttcggt caccagaccc gttactggaa cccgaaaatg aagccgttca
     9901 tcttcggtgc gcgtaacaaa gttcacatca tcaaccttga gaaaactgta ccgatgttca
     9961 acgaagctct ggctgaactg aacaagattg cttctcgcaa aggtaaaatc cttttcgttg
    10021 gtactaaacg cgctgcaagc gaagcggtga aagacgctgc tctgagctgc gaccagttct
    10081 tcgtgaacca tcgctggctg ggcggtatgc tgactaactg gaaaaccgtt cgtcagtcca
    10141 tcaaacgtct gaaagacctg gaaactcagt ctcaggacgg tactttcgaa aagctgacca
    10201 agaaagaagc gctgatgcgc actcgtgagc tggagaaact ggaaaacagc ctgggcggta
    10261 tcaaagacat gggcggtctg ccggacgctc tgtttgtaat cgatgctgac cacgaacaca
    10321 ttgctatcaa agaagcaaac aacctgggta ttccggtatt tgctatcgtt gataccaact
    10381 ctgatccgga cggtgttgac ttcgttatcc cgggtaacga cgacgcaatc cgtgctgtga
    10441 ccctgtacct gggcgctgtt gctgcaaccg tacgtgaagg ccgttctcag gatctggctt
    10501 cccaggcgga agaaagcttc gtagaagctg agtaataagg cttgataact cccccaaaat
    10561 agttcgagtt gcagaaaggc ggcaagctcg agaattcccg ggagcttaca tcagtaagtg
    10621 accgggatga gcgagcgaag acaacgcatc tgcggcgcga aatatgaagg gggagagccc
    10681 ttatagacca ggtagtacac gtttggttag ggggcctgca tatggccccc tttttcactt
    10741 ttatatctgt gcggtttaat gccgggcaga tcacatctcc gaggatttta gaatggctga
    10801 aattaccgca tccctggtaa aagagctgcg tgagcgtact ggcgcaggca tgatggattg
    10861 caaaaaagca ctgactgaag ctaacggcga catcgagctg gcaatcgaaa acatgcgtaa
    10921 gtccggtgct attaaagcag cgaaaaaagc aggcaacgtt gctgctgacg gcgtgatcaa
    10981 aaccaaaatc gacggcaact acggcatcat tctggaagtt aactgccaga ctgacttcgt
    11041 tgcaaaagac gctggtttcc aggcgttcgc agacaaagtt ctggacgcag ctgttgctgg
    11101 caaaatcact gacgttgaag ttctgaaagc acagttcgaa gaagaacgtg ttgcgctggt
    11161 agcgaaaatt ggtgagaaca tcaacattcg ccgcgttgct gcgctggaag gcgacgttct
    11221 gggttcttat cagcacggtg cgcgtatcgg cgttctggtt gctgctaaag gcgctgacga
    11281 agagctggtt aaacacatcg ctatgcacgt tgctgcaagc aagccagaat tcatcaaacc
    11341 agaagacgta tccgctgaag tggtagaaaa agaataccag gtacagctgg atatcgcgat
    11401 gcagtctggt aagccgaaag aaatcgcaga gaaaatggtt gaaggccgca tgaagaaatt
    11461 caccggcgaa gtttctctga ccggtcagcc gttcgttatg gaaccaagca aaactgttgg
    11521 tcagctgctg aaagagcata acgctgaagt gactggcttc atccgcttcg aagtggggga
    11581 aggcatcgag aaagttgaga ctgactttgc agcagaagtt gctgcgatgt ccaagcagtc
    11641 ttaattatcg aaaaggagcc gcctgagggc ggcttctttt tgtgcccatc ttgtaaattc
    11701 agctaaccct tgtggggctg cgctgaaaag cgacgtacaa tgtcgctagt attaattcat
    11761 ttcaatcgtt gacagtctca ggaaagaaac atggctacca atgcaaaacc cgtctataaa
    11821 cgcattctgc ttaagttgag tggcgaagct ctgcagggca ctgaaggctt cggtattgat
    11881 gcaagcatac tggatcgtat ggctcaggaa atcaaagaac tggttgaact gggtattcag
    11941 gttggtgtgg tgattggtgg gggtaacctg ttccgtggcg ctggtctggc gaaagcgggt
    12001 atgaaccgcg ttgtgggcga ccacatgggg atgctggcga ccgtaatgaa cggcctggca
    12061 atgcgtgatg cactgcaccg cgcctatgtg aacgctcgtc tgatgtccgc tattccattg
    12121 aatggcgtgt gcgacagcta cagctgggca gaagctatca gcctgttgcg caacaaccgt
    12181 gtggtgatcc tctccgccgg tacaggtaac ccgttcttta ccaccgactc agcagcttgc
    12241 ctgcgtggta tcgaaattga agccgatgtg gtgctgaaag caaccaaagt tgacggcgtg
    12301 tttaccgctg atccggcgaa agatccaacc gcaaccatgt acgagcaact gacttacagc
    12361 gaagtgctgg aaaaagagct gaaagtcatg gacctggcgg ccttcacgct ggctcgtgac
    12421 cataaattac cgattcgtgt tttcaatatg aacaaaccgg gtgcgctgcg ccgtgtggta
    12481 atgggtgaaa aagaagggac tttaatcacg gaataattcc cgtgatggat aaataagggt
    12541 aagattccgc gtaagtatcg cgggggcgta agtctggtta taaggcgtta ttgttgcagg
    12601 cagtttggtc acggccagcg cgcagcaacc ggagcgtaca aaagtacgtg aggatggcga
    12661 gcactgctcg gggccaaaat ggcaaataaa atagcctaat aatccagacg attacccgta
    12721 atatgtttaa tcagggctat acttagcaca cttccactgt gtgtgactgt ctggtctgac
    12781 tgagacaagt tttcaaggat tcgtaacgtg attagcgata tcagaaaaga tgctgaagta
    12841 cgcatggaca aatgcgtaga agcgttcaaa acccaaatca gcaaaatacg cacgggtcgt
    12901 gcttctccca gcctgctgga tggcattgtc gtggaatatt acggcacgcc gacgccgctg
    12961 cgtcagctgg caagcgtaac ggtagaagat tcccgtactc tgaaaatcaa cgtgtttgat
    13021 cgttcaatgt ctccggccgt tgaaaaagcg attatggcgt ccgatcttgg cctgaacccg
    13081 aactctgcgg gtagcgacat ccgtgttccg ctgccgccgc tgacggaaga acgtcgtaaa
    13141 gatctgacca aaatcgttcg tggtgaagca gaacaagcgc gtgttgcagt acgtaacgtg
    13201 cgtcgtgacg cgaacgacaa agtgaaagca ctgttgaaag ataaagagat cagcgaagac
    13261 gacgatcgcc gttctcagga cgatgtacag aaactgactg atgctgcaat caagaaaatt
    13321 gaagcggcgc tggcagacaa agaagcagaa ctgatgcagt tctgatttct tgaacgacaa
    13381 aaacgccgct cagtagatcc ttgcggatcg gctggcggcg ttttgctttt tattctgtct
    13441 caactctgga tgtttcatga agcaactcac cattctgggc tcgaccggct cgattggttg
    13501 cagcacgctg gacgtggtgc gccataatcc cgaacacttc cgcgtagttg cgctggtggc
    13561 aggcaaaaat gtcactcgca tggtagaaca gtgcctggaa ttctctcccc gctatgccgt
    13621 aatggacgat gaagcgagtg cgaaacttct taaaacgatg ctacagcaac agggtagccg
    13681 caccgaagtc ttaagtgggc aacaagccgc ttgcgatatg gcagcgcttg aggatgttga
    13741 tcaggtgatg gcagccattg ttggcgctgc tgggctgtta cctacgcttg ctgcgatccg
    13801 cgcgggtaaa accattttgc tggccaataa agaatcactg gttacctgcg gacgtctgtt
    13861 tatggacgcc gtaaagcaga gcaaagcgca attgttaccg gtcgatagcg aacataacgc
    13921 catttttcag agtttaccgc aacctatcca gcataatctg ggatacgctg accttgagca
    13981 aaatggcgtg gtgtccattt tacttaccgg gtctggtggc cctttccgtg agacgccatt
    14041 gcgcgatttg gcaacaatga cgcccgatca agcctgccgt catccgaact ggtcgatggg
    14101 acgtaaaatt tctgtcgatt cggctaccat gatgaacaaa ggtctggaat acattgaagc
    14161 gcgttggctg tttaacgcca gcgccagcca gatggaagtg ctgattcacc cgcagtcagt
    14221 gattcactca atggtgcgct atcaggacgg cagtgttctg gcgcagctgg gggaaccgga
    14281 tatgcgtacg ccaattgccc acaccatggc atggccgaat cgcgtgaact ctggcgtgaa
    14341 gccgctcgat ttttgcaaac taagtgcgtt gacatttgcc gcaccggatt atgatcgtta
    14401 tccatgcctg aaactggcga tggaggcgtt cgaacaaggt caggcagcga cgacagcatt
    14461 gaatgccgca aacgaaatca ccgttgctgc ttttcttgcg caacaaatcc gctttacgga
    14521 tatcgctgcg ttgaatttat ctgtactgga aaaaatggat atgcgcgaac cacaatgtgt
    14581 ggacgatgtg ttatctgttg atgcgaacgc gcgtgaagtc gccagaaaag aggtgatgcg
    14641 tctcgcaagc tgaggataat ccggctacag agagtcgcgc tatttgttag cgtagggctt
    14701 cagtgatata gtctgcgcca tctgatcgta agtagttggc tttataaggt caggtatgcc
    14761 gtggttttac acggcttttt tttgtatagg cttcagtatt cctgagtacc gtaaaccctg
    14821 tcagggaata aaaaacgcgt gatgttgtct gctactcaac cacttagcga aaaattgcca
    14881 gcgcatggct gccgtcatgt tgcgatcatt atggacggca atggtcgctg ggcaaaaaag
    14941 caagggagga ttcgtgcctt tgggcataaa gccggggcaa aatccgtccg ccgggctgtc
    15001 tcttttgcgg ccaacaacgg tattgaggcg ttaacgctgt atgcctttag tagtgaaaac
    15061 tggaaccgac cagcgcagga agtcagtgcg ttaatggaac tgtttgtgtg ggcgctcgat
    15121 agcgaagtaa aaagtctgca ccgacataac gtgcgtctgc gtattattgg cgataccagt
    15181 cgctttaact cgcgtttgca agaacgtatt cgtaaatctg aagcgctaac agctgggaat
    15241 accggtctga cgctgaatat tgcggcgaac tacggtggac gttgggatat agtccaggga
    15301 gtcaggcaac tggctgaaaa ggtgcagcaa ggaaacctgc aaccagatca gatagatgaa
    15361 gagatgctaa accagcatgt ctgtatgcat gaactggccc ctgtagattt agtaattagg
    15421 actggggggg agcatcgcat tagtaacttt ttgctttggc aaattgccta tgccgaactt
    15481 tactttacag atgttctctg gcccgatttc gatgaacaag actttgaagg ggcgttaaat
    15541 gcctttgcta atcgagagcg tcgtttcggc ggcaccgagc ccggtgatga aacagcctga
    15601 tggggggcgc ttttgctgaa gtatcgcctg atatctgctt ttgtgttaat acccgtcgtc
    15661 atcgcggcgt tgtttctgtt gccgccggtg gggttcgcca tcgtaacgct ggtggtctgc
    15721 atgctggcag cgtgggaatg gggacagctt agcggtttta ccactcgttc gcagcgagta
    15781 tggttggcgg tgttatgcgg gttattgttg gcgctgatgc tttttctgtt gccggaatat
    15841 caccgaaata ttcatcaacc gctggttgaa atctcacttt gggcttcgtt gggttggtgg
    15901 attgtcgcgc tattgctggt gctgttttac ccaggttccg cagcaatctg gcgtaactct
    15961 aaaacattgc gccttatttt tggcgtgcta accattgttc ccttcttctg gggcatgctg
    16021 gcgttacggg cctggcacta tgacgagaat cattacagtg gcgcaatatg gctgctctat
    16081 gtcatgatcc tggtatgggg cgctgactcc ggcgcatata tgtttggcaa attgtttggt
    16141 aaacataagc tggcaccgaa ggtttctccg ggtaaaacct ggcaaggctt tataggtgga
    16201 ctagcgaccg cagcggtaat ctcatggggt tatggcatgt gggcgaatct cgacgtcgct
    16261 cccgtcacct tactcatttg ctctattgtc gcagcgttag cctcagtgct cggcgatctg
    16321 accgagagta tgtttaagcg tgaagcagga attaaggaca gcggtcattt aattccagga
    16381 cacggtggta ttttagatcg tattgatagc ctgacggctg cggtaccggt ctttgcttgc
    16441 ttgttgttac tggtattcag gacgctttaa cggaaggtaa tatgctgagt tttctctggg
    16501 atttggcttc gttcatcgtt gcactgggtg tacttatcac cgtgcatgaa tttggtcatt
    16561 tctgggttgc ccggcgttgt ggtgttcgcg ttgagcgttt ctcaataggg tttggtaagg
    16621 cgctctggcg gcgaactgat aagctcggca ccgaatatgt tatggccctg attccgctgg
    16681 gcggttatgt caaaatgctg gatgagcgcg cagaaccggt cgttccggaa ctccgccacc
    16741 atgccttcaa taataaatct gttggccaac gagcggcgat tattgccgca ggtccggttg
    16801 caaacttcat ttttgctatc tttgcctact ggctggtttt tattattggt gtgcctggcg
    16861 tacgtccggt ggttggtgaa atagcagcca attcgatagc tgcggaagca caaattgcac
    16921 caggtacgga actaaaagcc gtagatggta tcgaaacgcc tgattgggat gccgtgcgtt
    16981 tgcagttggt cgataaaatt ggcgatgaaa gcaccaccat tacagtagcg ccatttggca
    17041 gcgaccaacg gcgggatgta aagctcgatt tacgtcactg ggcgtttgag cctgataaag
    17101 aagatccggt atcttcgctg gggattcgtc ctcgtgggcc gcaaattgaa cctgtactgg
    17161 aaaatgtgca gccaaactcg gcggcaagca aggcaggttt gcaagcaggc gacaggatcg
    17221 ttaaagtcga tggtcagccc ttaacgcagt gggtgacctt tgtgatgctt gtccgggata
    17281 acccgggtaa atccttagcg ttagaaatcg aaaggcaggg gagtcccttg tctttgacat
    17341 taatcccgga gagtaaaccg ggtaatggta aagcgattgg ttttgtcggt attgagccga
    17401 aagtcattcc tttgccagat gagtataaag ttgtacgcca gtatgggccg ttcaacgcca
    17461 tcgtcgaagc cacggacaaa acgtggcagc tgatgaagct gacggtcagt atgctgggaa
    17521 aattgatcac cggtgatgtg aaactgaaca acctcagtgg gccgatctct atcgccaagg
    17581 gggctgggat gacagcggaa ctcggggtag tttattacct gccgtttctt gcgcttatta
    17641 gcgtgaactt agggataatt aacctgtttc cgttgcccgt acttgacggg gggcatctgc
    17701 tgttccttgc gatcgaaaag atcaagggcg gaccggtatc cgagcgggtt caagactttt
    17761 gttatcgcat tggctcgatt ctgctggtgc tgttaatggg gcttgcactt ttcaatgatt
    17821 tctctcggtt atgagagtta gttaggaaga acgcataata acgatggcga tgaaaaagtt
    17881 gctcatagcg tcgctgctgt ttagcagcgc caccgtatac ggtgctgaag ggttcgtagt
    17941 gaaagatatt catttcgaag gccttcagcg tgtcgccgtt ggtgcggcac tcctcagtat
    18001 gccggtgcgc acaggcgaca cggttaatga tgaagatatc agtaatacca ttcgcgctct
    18061 gtttgctacc ggcaactttg aggatgttcg cgtccttcgt gatggtgata cccttctggt
    18121 tcaggtaaaa gaacgtccga ccattgccag cattactttc tccggtaaca aatcggtgaa
    18181 agatgacatg ctgaagcaaa acctcgaggc ttctggtgtg cgtgtgggcg aatccctcga
    18241 tcgcaccacc attgccgata tcgagaaagg tctggaagac ttctactaca gcgtcggtaa
    18301 atatagcgcc agcgtaaaag ctgtcgtgac cccgctgccg cgcaaccgtg ttgacctaaa
    18361 actggtgttc caggaaggtg tgtcagctga aatccagcaa attaacattg ttggtaacca
    18421 tgctttcacc accgacgaac tgatctctca tttccaactg cgtgacgaag tgccgtggtg
    18481 gaacgtggta ggcgatcgta aataccagaa acagaaactg gcgggcgacc ttgaaaccct
    18541 gcgcagctac tatctggatc gcggttatgc ccgtttcaac atcgactcta cccaggtcag
    18601 tctgacgcca gataaaaaag gtatttacgt cacggtgaac attaccgaag gcgatcagta
    18661 caagctttct ggcgttgaag tgagcggcaa ccttgccggg cactccgctg aaattgagca
    18721 gctgactaag atcgagccgg gcgagctgta taacggcacc aaagtgacca agatggaaga
    18781 tgacatcaaa aagcttctcg gtcgctatgg ttatgcctat ccgcgcgtac agtcgatgcc
    18841 tgaaattaac gatgccgaca aaaccgttaa attacgcgtg aacgttgatg cgggtaaccg
    18901 tttctacgtg cgtaagatcc gttttgaagg taacgatacc tcgaaagatg ccgtcctgcg
    18961 tcgcgaaatg cgtcagatgg aaggtgcatg gctgggaagc gatctggtcg atcagggtaa
    19021 ggagcgtctg aatcgtctgg gcttctttga aactgtcgat accgataccc aacgtgttcc
    19081 gggtagcccg gaccaggtag atgtagtcta caaggtgaaa gaacgtaaca ccggtagctt
    19141 caacttcggg attggttacg gtactgaaag tggcgtgagc ttccaggctg gcgtgcaaca
    19201 ggataactgg ttaggtacag gttatgctgt tggtatcaac gggaccaaaa acgattacca
    19261 gacctatgct gaattgtcgg taaccaaccc gtacttcact gtagatggtg taagcctcgg
    19321 tggtcgtctc ttctataatg acttccaggc agatgacgcc gacctgtccg actataccaa
    19381 caagagttat ggtacagacg tgacgttggg cttcccgatc aacgaatata actcgctgcg
    19441 tgcaggtctg ggttatgtac ataactccct gtccaacatg cagcctcagg ttgcgatgtg
    19501 gcgttatctg tactctatgg gtgaacatcc gagcacctct gatcaggata acagcttcaa
    19561 aacggacgac ttcacgttca actatggttg gacctataac aagcttgacc gtggttactt
    19621 cccgacagat ggttcacgtg tcaacctgac cggtaaagtg accattcctg gatcggataa
    19681 cgaatactac aaagtgacgt tagacacggc gacttatgtg ccgatcgatg acgatcacaa
    19741 atgggttgtt ctggggcgta cccgctgggg ttatggtgat ggtttaggcg gcaaagagat
    19801 gccgttctac gagaacttct atgccggtgg ttccagcacc gtgcgtggct tccagtccaa
    19861 tacaattggt ccgaaagcag tttacttccc gcatcaggcc agtaattatg atccggacta
    19921 tgattacgaa tgtgcgactc aggacggcgc gaaagacctg tgtaaatcgg atgatgctgt
    19981 aggcggtaac gccatggcgg ttgccagcct cgagttcatc accccgacgc cgtttattag
    20041 cgataagtat gctaactcgg ttcgtacttc cttcttctgg gatatgggta ccgtttggga
    20101 tacaaactgg gattccagcc aatattctgg atatccggac tatagtgatc caagcaatat
    20161 ccgtatgtct gcgggtatcg cattacaatg gatgtcccca ttggggccgt tggtgttctc
    20221 ctacgcccag ccgttcaaaa agtacgatgg agacaaggca gaacagttcc agtttaacat
    20281 cggtaaaacc tggtaagtgt tctccacaaa ggaatgtagt ggtagtgtag cgatgacttt
    20341 aggcgatcaa tataagatcg ccgggccacg caaagaactg caccctccgg tgcaaatggg
    20401 atggtaagga gtttattgtg aaaaagtggt tattagcggc aggtctcggt ttagcactgg
    20461 caacttctgc tcaggcggct gacaaaattg caatcgtcaa tatgggcagc ctgttccagc
    20521 aggtagcgca gaaaaccggt gtttctaaca cgctggaaaa tgagttcaaa ggccgtgcca
    20581 gcgaactgca gcgtatggaa accgatctgc aggctaaaat gaaaaagctg cagtccatga
    20641 aagcgggcag cgatcgcact aagctggaaa aagacgtgat ggctcagcgc cagacttttg
    20701 ctcagaaagc gcaggctttt gagcaggatc gcgcacgtcg ttccaacgaa gaacgcggca
    20761 aactggttac tcgtatccag actgctgtga aatccgttgc caacagccag gatatcgatc
    20821 tggttgttga tgcaaacgcc gttgcttaca acagcagcga tgtaaaagac atcactgccg
    20881 acgtactgaa acaggttaaa taagtaatgc cttcaattcg actggctgat ttagcgcagc
    20941 agttggatgc agaactacac ggtgatggcg atatcgtcat caccggcgtt gcgtccatgc
    21001 aatctgcaca aacaggtcac attacgttca tggttaaccc aaaataccgt gagcatttag
    21061 gcttgtgcca ggcgtccgcg gttgtcatga cccaggacga tcttcctttc gcgaaaagtg
    21121 ccgcgctggt agtgaagaat ccctacctga cttacgcgcg catggcgcaa attttagata
    21181 ccacgccgca gcccgcgcag aacattgcac ccagtgcggt gatcgacgcg acggcgaagc
    21241 tgggtaacaa cgtatcgatt ggcgctaacg cggtgattga gtccggcgtt gaactgggcg
    21301 ataacgtgat tatcggtgcc ggttgcttcg taggtaaaaa cagcaaaatc ggtgcaggtt
    21361 cgcgtctctg ggcgaacgta accatttacc atgagatcca gatcggtcag aattgcctga
    21421 tccagtccgg aacagtggta ggcgcagacg gctttggtta tgccaacgat cgtggtaact
    21481 gggtgaagat cccacagatt ggtcgcgtaa ttattggcga tcgcgtggag atcggtgcct
    21541 gcacaaccat cgatcgcggc gcgctggatg acactgttat tggcaatggc gtgatcattg
    21601 ataaccagtg ccagattgca cataacgtcg tgattggcga caatacggcg gttgccggtg
    21661 gcgtcattat ggcgggcagc ctgaaaattg gtcgttactg catgatcggc ggagccagcg
    21721 taatcaacgg gcatatggaa atatgcgaca aagtgacggt tacgggcatg ggtatggtga
    21781 tgcgtcccat cactgaacca ggcgtctatt cctcaggcat tccgctgcaa cccaacaaag
    21841 tctggcgcaa aaccgctgca ctggtgatga acattgatga catgagcaag cgtctgaaat
    21901 cgcttgagcg caaggttaat caacaagact aacgttccat cttttgttcg ccaaacttta
    21961 cggcctgtct cattcttacg attgcggcag gccgtgttat tattgtcgtt tcttatattt
    22021 tgacaggaag agtatcttga ctactaacac tcatactctg cagattgaag agattttaga
    22081 acttctgccg caccgtttcc cgttcttact ggtggatcgc gtgctggatt ttgaagaagg
    22141 tcgttttctg cgcgcagtaa aaaatgtctc tgtcaatgag ccattcttcc agggccattt
    22201 ccctggaaaa ccgattttcc cgggtgtgct gattctggaa gcaatggcac aggcaacagg
    22261 tattctggcg tttaaaagcg taggaaaact ggaaccgggt gagctgtact acttcgctgg
    22321 tattgacgaa gcgcgcttca agcgcccggt cgtgcctggc gatcaaatga tcatggaagt
    22381 cactttcgaa aaaacgcgcc gcggcctgac ccgttttaaa ggggttgctc tggtcgatgg
    22441 taaagtagtt tgcgaagcaa cgatgatgtg tgctcgtagc cgggaggcct gatacgtgat
    22501 tgataaatcc gcctttgtgc atccaaccgc cattgtggaa gagggcgcgt caattggcgc
    22561 gaacgcacac attggtcctt tttgtatcgt tggaccccat gtcgaaattg gtgagggtac
    22621 cgtactgaaa tctcacgttg tcgtgaatgg tcatactaaa attggccgcg ataatgagat
    22681 ttatcagttc gcctccatcg gcgaagttaa ccaggatctg aaatatgctg gcgaaccgac
    22741 ccgtgtggaa atcggcgatc gtaaccgcat tcgcgaaagc gtcaccattc atcgtggcac
    22801 agtccagggc ggtggattga cgaaggtggg cagcgacaac ttactgatga tcaacgcgca
    22861 cattgcgcac gattgtacgg taggtaaccg ctgtattctc gccaacaacg caacgctggc
    22921 gggtcacgta tcggttgacg acttcgcgat catcggcggc atgaccgcag tccatcagtt
    22981 ctgcatcatt ggtgcgcacg tgatggttgg cggctgctcc ggtgtggcgc aggacgtccc
    23041 tccttatgtc attgcgcagg gtaaccacgc aacgccgttc ggtgtcaata tcgaagggct
    23101 gaagcgccgc ggattcagcc gtgaggcgat taccgctatc cgcaatgcgt ataagctgat
    23161 ttatcgtagc ggtaaaacgc tcgatgaagt gaaaccggaa attgctgaac tggcggaaac
    23221 atatccggaa gtgaaagcct ttaccgattt ctttgcacgc tcaacgcgcg gtctgattcg
    23281 ttaatgactg aacagcgtcc attaacgatt gccctggtcg ccggagaaac ctccggcgat
    23341 atcctggggg ccggtttaat ccgcgctctg aaagaacatg tgcccaacgc ccgctttgtt
    23401 ggtgttgccg ggccacgaat gcaggctgaa ggctgcgaag cttggtacga aatggaagaa
    23461 ctggcggtaa tgggcattgt tgaagtgctc ggtcgtctgc gtcgcttact gcatattcgt
    23521 gccgatctga caaagcgttt tggcgaactg aagccagatg tttttgttgg tattgatgcg
    23581 cccgacttca atattactct tgaaggtaac ctcaaaaagc agggtatcaa aaccattcat
    23641 tacgtcagtc catccgtctg ggcgtggcga cagaaacgcg ttttcaaaat aggcagagcc
    23701 accgatctgg tgctcgcatt tctgcctttc gaaaaagcgt tttatgacaa atacaatgta
    23761 ccgtgccgct ttatcggtca taccatggct gatgccatgc cgttagatcc agataaaaat
    23821 gccgcccgtg atgtgctggg gatccctcac gatgcccact gcctggcgtt gctaccgggg
    23881 agccgtggtg cagaagttga aatgcttagt gccgatttcc tgaaaacggc ccagcttttg
    23941 cgccagacat atccggatct cgaaatcgtg gtgccactgg tgaatgccaa acgccgcgag
    24001 cagtttgaac gcatcaaagc tgaagtcgcg ccagaccttt cagttcattt gctggatggg
    24061 atgggccgtg aggcgatggt cgccagcgat gcggcgctac tggcgtcggg tacggcagcc
    24121 ctggagtgta tgctggcgaa atgcccgatg gtggtgggat atcgcatgaa gccttttacc
    24181 ttctggttgg cgaagcggct ggtgaaaact gattatgtct cgctgccaaa tctgctggcg
    24241 ggcagagagt tagttaaaga attattgcag gaagagtgtg agccgcaaaa actggctgcg
    24301 gcgctgttac cgctgttggc gaacgggaaa accagccacg cgatgcacga taccttccgt
    24361 gaactgcatc agcagatccg ctgcaatgcc gatgagcagg cggcacaagc cgttctggag
    24421 ttagcacaat gatcgaattt gtttatccgc acacgcagct ggttgcgggt gtggatgaag
    24481 tcggacgcgg gccgttagtt ggcgcggtcg tcaccgctgc ggtgatcctt gacccggcgc
    24541 gcccgattgc cgggctgaat gattccaaaa agctgagcga aaaacgccgt ctggcgctct
    24601 atgaagagat caaagagaaa gcgttgagct ggagtctggg ccgcgcggaa ccccacgaaa
    24661 tcgacgagct gaacattctt catgcgacca tgctggcgat gcagcgtgcc gtcgctgggc
    24721 tgcatattgc gccggaatat gtgttgattg atggtaaccg ctgcccgaaa ttaccgatgc
    24781 ctgcgatggc tgtggtgaaa ggcgatagcc gcgtaccgga aatcagtgcc gcgtctatcc
    24841 tggcgaaagt gacgcgtgac gccgaaatgg cggcgctgga tattgttttc ccgcaatatg
    24901 gttttgccca acacaaaggg tacccaaccg cttttcatct ggaaaaactg gctgaacacg
    24961 gcgcgaccga acaccatcgg cgcagctttg ggcctgtcaa acgcgcactg ggacttgcgt
    25021 cctgattctt gtgtcgagat taagtaaacc ggaatctgaa gatgtctgaa ccacgtttcg
    25081 tacacctgcg ggtgcacagc gactactcga tgatcgatgg cctggccaaa accgcaccgt
    25141 tggtaaaaaa ggcggcggcg ttgggtatgc cagcactggc gatcaccgat ttcaccaacc
    25201 tttgtggtct ggtgaagttc tacggagcgg gacatggcgc agggattaag cctatcgtcg
    25261 gggcagattt taacgtccag tgcgacctgc tgggtgatga gttaacccac ctgacggtac
    25321 tggcggcgaa caataccggc tatcagaatc tgacgttgct gatctcaaaa gcgtatcagc
    25381 gcgggtacgg tgccgccggg ccgatcatcg atcgcgactg gcttatcgaa ttaaacgaag
    25441 ggttgatcct tctttccggc ggacgcatgg gcgacgtcgg acgcagtctt ttgcgtggta
    25501 acagcgcgct ggtagatgag tgtgtcgcgt tttatgaaga acacttcccg gatcgctatt
    25561 ttcttgagct gatccgcacc ggcaggccgg acgaagaaag ctatctgcac gcggcggtgg
    25621 aactggcgga agcgcgcggt ttgcccgtcg tggcgaccaa cgacgtgcgc tttatcgaca
    25681 gcagcgactt tgacgcacac gaaatccgcg tcgcgatcca cgacggcttt accctcgacg
    25741 atcctaaacg cccgcgtaac tattcgccgc agcaatatat gcgtagcgaa gaggagatgt
    25801 gcgagctgtt tgccgacatc cccgaagctc ttgccaacac cgttgagatc gccaaacgtt
    25861 gtaacgtaac cgtgcgtctt ggtgaatact tcctgccgca gttcccgacc ggggacatga
    25921 gcaccgaaga ttatctggtc aagcgtgcaa aagagggcct ggaagagcgt ctggcctttt
    25981 tattccctga cgaggaagaa cgtgttaagc gccgcccgga atatgacgaa cgtctggaga
    26041 ctgaacttca ggttatcaac cagatgggct tcccgggcta cttcctcatc gttatggaat
    26101 ttatccagtg gtcgaaagat aacggcgtac cggtagggcc aggccgtggc tccggtgcgg
    26161 gttcactggt ggcctacgcg ctgaaaatca ccgacctcga tccgctggaa tttgacctgc
    26221 tgttcgaacg tttccttaac ccggaacgtg tctccatgcc tgacttcgac gttgacttct
    26281 gtatggagaa acgcgatcag gttatcgagc acgtagcgga catgtacggt cgtgatgcgg
    26341 tatcgcagat catcaccttc ggtacaatgg cggcgaaagc ggtgatccgc gacgtaggcc
    26401 gcgtgctggg gcatccgtac ggctttgtcg atcgtatctc gaaactgatc ccgcccgatc
    26461 cggggatgac gctggcgaaa gcgtttgaag ccgagccgca gctgccggaa atctacgaag
    26521 cggatgaaga agttaaggcg ctgatcgaca tggcgcgcaa actggaaggg gtcacccgta
    26581 acgccggtaa gcacgccggt ggggtggtta tcgcgccgac caaaattacc gattttgcgc
    26641 cgctttactg cgatgaagag ggcaaacatc cggtcaccca gtttgataaa agcgacgttg
    26701 aatacgccgg actggtgaag ttcgacttcc ttggtttgcg tacgctcacc atcatcaact
    26761 gggcgctgga gatgatcaac aagcggcggg cgaagaatgg cgagccgccg ctggatatcg
    26821 ctgcgatccc gctggatgat aagaaaagct tcgacatgct gcaacgctcg gaaaccacgg
    26881 cggtattcca gcttgaatcg cgcggcatga aggacctgat caagcgtctg caacctgact
    26941 gcttcgaaga tatgatcgca ctggtggcac tgttccgccc cggcccgttg cagtcaggga
    27001 tggtggataa ctttatcgac cgtaaacatg gtcgcgaaga gatttcctat ccggacgtac
    27061 agtggcagca tgaaagcctg aaaccggtac tggagccaac ctacggcatc atcctgtatc
    27121 aggaacaggt catgcagatt gcgcaggtgc tttctggtta taccctcggt ggcgcggata
    27181 tgctgcgtcg tgcgatgggt aagaaaaagc cggaagagat ggctaagcag cgttctgtat
    27241 ttgctgaagg tgcagaaaag aacggaatca acgccgaact ggcgatgaaa atcttcgacc
    27301 tggtggagaa attcgcgggt tacggattta acaaatcgca ctctgcggcc tatgctttgg
    27361 tgtcatatca aacgttatgg ctgaaagcgc actatccggc ggagtttatg gcggcggtaa
    27421 tgaccgccga tatggacaac accgagaagg tggtgggcct ggtggatgag tgctggcgga
    27481 tggggctgaa aatcctgcca ccagatataa actccggtct ttaccatttc cacgtcaacg
    27541 acgacggcga aatcgtgtat ggtattggcg ccatcaaagg ggtaggtgaa ggtccgattg
    27601 aggccatcat cgaagcccgt aataaaggcg gctacttccg cgaactgttt gatctctgcg
    27661 cccgtaccga caccaaaaag ttaaaccggc gggtgctgga aaaactgatc atgtccgggg
    27721 cgtttgaccg tcttgggcca caccgcgcgg cgctgatgaa ctcgctgggt gatgcgttaa
    27781 aagcggcaga tcaacacgcg aaagcggaag ctatcggtca ggccgatatg ttcggcgtgc
    27841 tggccgaaga gccggaacaa attgaacaat cctacgccag ctgccaaccg tggccggagc
    27901 aggtggtgtt agatggggaa cgtgaaacgt taggtctgta ccttacggga caccctatca
    27961 accagtattt aaaagagatt gagcgttatg tcggaggcgt aaggctgaaa gacatgcacc
    28021 cgacagaacg tggtaaagtc atcacggctg cggggctcgt tgttgccgcg cgggttatgg
    28081 tcaccaagcg cggcaatcgt atcggtatct gcacgctgga tgaccgttcc gggcggctgg
    28141 aagtgatgtt gtttactgac gccctggata aataccagca attgctggaa aaagaccgca
    28201 tacttatcgt cagcggacag gtcagctttg atgacttcag cggtgggctt aaaatgaccg
    28261 ctcgcgaagt gatggatatt gacgaagccc gggaaaaata tgctcgcggg cttgctatct
    28321 cgctgacgga caggcaaatt gatgaccagc ttttaaaccg actccgtcag tctctggaag
    28381 cccaccgctc tgggacaatt ccagtacatc tctactatca gagggcggat gcacgcgcgc
    28441 ggttgcgttt tggcgcgacg tggcgtgtct ctccgagcga tcgtttatta aacgatctcc
    28501 gtggcctcat tggttcggag caggtggaac tggagtttga ctaatacagg aatactatga
    28561 gtctgaattt ccttgatttt gaacagccga ttgcagagct ggaagcgaaa atcgattctc
    28621 tgactgcggt tagccgtcag gatgagaaac tggatattaa catcgatgaa gaagtgcatc
    28681 gtctgcgtga aaaaagcgta gaactgacac gtaaaatctt cgccgatctc ggtgcatggc
    28741 agattgcgca actggcacgc catccacagc gtccttatac cctggattac gttcgcctgg
    28801 catttgatga atttgacgaa ctggctggcg accgcgcgta tgcagacgat aaagctatcg
    28861 tcggtggtat cgcccgtctt gacggtcgtc cggtgatgat cattggtcat caaaaaggtc
    28921 gtgaaaccaa agaaaaaatt cgccgtaact ttggtatgcc agcgccagaa ggttaccgca
    28981 aagcactgcg tctgatgcaa atggctgaac gctttaagat gcctatcatc acctttatcg
    29041 acaccccggg ggcttatcct ggcgtgggcg cagaagagcg tggtcagtct gaagccattg
    29101 cacgcaacct gcgtgaaatg tctcgcctcg gcgtaccggt agtttgtacg gttatcggtg
    29161 aaggtggttc tggcggtgcg ctggcgattg gcgtgggcga taaagtgaat atgctgcaat
    29221 acagcaccta ttccgttatc tcgccggaag gttgtgcgtc cattctgtgg aagagcgccg
    29281 acaaagcgcc gctggcggct gaagcgatgg gtatcatcgc tccgcgtctg aaagagctga
    29341 aactgatcga ctccatcatc ccggaaccgc tgggcggcgc tcaccgtaac ccggaagcga
    29401 tggcggtatc gttgaaagcg caactgctgg cggatctggc cgatctcgat gtgttaagca
    29461 ctgaagattt aaaaaatcgt cgttatcagc gcctgatgag ctacggttac gcgtaattcg
    29521 caaaagttct gaaaaagggt cacttcgg
//
LOCUS       DAZPDU010000037        28894 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZPDU010000037 DAZPDU010000000
VERSION     DAZPDU010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28894
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.037"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..1262)
                     /gene="ipaHb"
                     /locus_tag="ACHURS_001490"
     CDS             complement(<1..1262)
                     /gene="ipaHb"
                     /locus_tag="ACHURS_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaHb"
                     /protein_id="HFR1237263.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRAFSAFLDRLSDTVSARNTSGFREQVAAWLEKLSASAELRQQSFTV
                     AADATESCEDRVALTWNNLRKTLLVHQAS"
     gene            1753..2310
                     /gene="pinE"
                     /locus_tag="ACHURS_001491"
                     /pseudo
     CDS             1753..2310
                     /gene="pinE"
                     /locus_tag="ACHURS_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
     gene            complement(2649..3497)
                     /locus_tag="ACHURS_001492"
     CDS             complement(2649..3497)
                     /locus_tag="ACHURS_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFR1237264.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            3706..4341
                     /gene="yfhb"
                     /locus_tag="ACHURS_001493"
     CDS             3706..4341
                     /gene="yfhb"
                     /locus_tag="ACHURS_001493"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFR1237265.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            4399..4902
                     /gene="tadA"
                     /locus_tag="ACHURS_001494"
     CDS             4399..4902
                     /gene="tadA"
                     /locus_tag="ACHURS_001494"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFR1237266.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQPSTD"
     gene            complement(4899..6455)
                     /gene="mltF"
                     /locus_tag="ACHURS_001495"
     CDS             complement(4899..6455)
                     /gene="mltF"
                     /locus_tag="ACHURS_001495"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFR1237267.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVSQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKDTKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDCTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            6713..10600
                     /gene="purL"
                     /locus_tag="ACHURS_001496"
                     /gene_synonym="purI"
     CDS             6713..10600
                     /gene="purL"
                     /locus_tag="ACHURS_001496"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFR1237268.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNTLLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            11158..12585
                     /gene="qseE"
                     /locus_tag="ACHURS_001497"
     CDS             11158..12585
                     /gene="qseE"
                     /locus_tag="ACHURS_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFR1237269.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            12750..13463
                     /gene="qseG"
                     /locus_tag="ACHURS_001498"
     CDS             12750..13463
                     /gene="qseG"
                     /locus_tag="ACHURS_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFR1237270.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPTGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            13453..14787
                     /gene="glrR"
                     /locus_tag="ACHURS_001499"
     CDS             13453..14787
                     /gene="glrR"
                     /locus_tag="ACHURS_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFR1237271.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            14848..15186
                     /gene="glnB"
                     /locus_tag="ACHURS_001500"
     CDS             14848..15186
                     /gene="glnB"
                     /locus_tag="ACHURS_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFR1237272.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(15231..16421)
                     /gene="hmpA"
                     /locus_tag="ACHURS_001501"
     CDS             complement(15231..16421)
                     /gene="hmpA"
                     /locus_tag="ACHURS_001501"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFR1237273.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWHRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            16749..18002
                     /gene="glyA"
                     /locus_tag="ACHURS_001502"
     CDS             16749..18002
                     /gene="glyA"
                     /locus_tag="ACHURS_001502"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFR1237274.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARFPVYA"
     gene            complement(18200..19393)
                     /locus_tag="ACHURS_001503"
                     /pseudo
     CDS             complement(18200..19393)
                     /locus_tag="ACHURS_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
     gene            19511..22788
                     /locus_tag="ACHURS_001504"
                     /pseudo
     CDS             19511..22788
                     /locus_tag="ACHURS_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            22885..23868
                     /locus_tag="ACHURS_001505"
     CDS             22885..23868
                     /locus_tag="ACHURS_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFR1237275.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTLGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLNKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            23891..25402
                     /locus_tag="ACHURS_001506"
     CDS             23891..25402
                     /locus_tag="ACHURS_001506"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFR1237276.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            25427..26425
                     /locus_tag="ACHURS_001507"
     CDS             25427..26425
                     /locus_tag="ACHURS_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1237277.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            26458..27552
                     /locus_tag="ACHURS_001508"
     CDS             26458..27552
                     /locus_tag="ACHURS_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFR1237278.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHCATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            27564..28436
                     /locus_tag="ACHURS_001509"
     CDS             27564..28436
                     /locus_tag="ACHURS_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFR1237279.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(28484..>28894)
                     /locus_tag="ACHURS_001510"
     CDS             complement(28484..>28894)
                     /locus_tag="ACHURS_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFR1237280.1"
                     /translation="RYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQYMAS
                     LGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDMTGDA
                     VGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
BASE COUNT         6866 a         7143 c         7864 g         7021 t
ORIGIN      
        1 gatgcctgat ggaccaggag ggttttccgg agattgttcc atgtgagcgc gacacggtcc
       61 tcacagctct cagtggcatc agcagcaaca gtgaaagact gctgtcgaag ctccgcagag
      121 gcactgagtt tttccagcca tgcagcgacc tgttcacgga atccggaggt attgcgtgca
      181 gagacggtat cggaaaggcg gtcaaggaac gcggagaatg cccgggaatt ctcctcggtg
      241 gaaaaagaag tccattgtgt agaaacctcc cgacgatctg cttcaggaaa ccaggctgcc
      301 acagtttcgg gaagaggggg gatctgaaag ttctgccccc cttcagtgat gactctgata
      361 tggcgcgaat gtaaattttg cggcaacatc tggcgtaaaa acagtctgtt ttggtctgac
      421 agggaaatac gaaactgagg cccgttaaaa tttggaaccg aagtttgctg tattaagaat
      481 agcagagttt gatctgataa ggggttattt gcaatctcaa tagttatatg aggatctaaa
      541 cgaaaaagat ttgtcgggat tgtagttatt tggttgtttt caaaataaaa aaatcttctc
      601 atgataatat tttcttggat gtttggtaag ttgcttaatc tattgctata tgcaaaaaga
      661 agttgcagtc tcctaggtaa agggggcagt tcagtaagat ggttgtgcgc aaccttaagt
      721 atcttaagat tagaaggcag agatggaaga gtttgcagtt cgttgttcct aacctcaagt
      781 ttttttaaat tcaagggtaa tgaaggcagt gtggataacc tattatactc tgcgcttagt
      841 gatttgatgg tgtctggtaa taacggcaga ttctctaatt gattttcgga cacatccaaa
      901 gttattagat aatgtggtaa tgcagatata tgtgttaaga aattatctct tgcaataagt
      961 tctttaatat tttcaggtaa atcagtaagg atggttaatt tattacagct cacatcaagc
     1021 tttttaatgc aagcagggag tacaggtaac gaggaaagcg ccaaggctcg caggtccagt
     1081 ttatgcccct tagaggtaag acatgcacga agtcttctta ccgcctcagt acgctgttct
     1141 tccggtaacc cctgtttctc ccaatcatcc cattcggaca aataatggtt gggggatgta
     1201 attgtgctga tatgtggaaa taaacaggat gaaatatttc tgagcatatt aatttctcgc
     1261 atatggatat ggaattagat tgtgaataaa ttgtatttaa tgtatctctc ctaatgagga
     1321 gggtgattca gaatattcgc cataagggaa tgagtgttat atcatgcttt ctaccctggt
     1381 aagtatattt gcttttttcg tatatttggt tcatcttttt ttgatgtttt acatattcac
     1441 aatgcagagg atttatacca gaagacatta gtcttgagta agatactaca acatctccaa
     1501 ctattttagc cagcctgact acttgacttg gtaatatttg ttgctaataa tcatgtataa
     1561 aataagctta tatacatagt aacttcagca aaggatttag taatgatcca aatcatatct
     1621 caatgtactg tttttttctt taaaaaaaca gtacattcta ctttataatg actgtgtatt
     1681 cccatggaag gagatcaccc aattctcccc cccgactttt gacgcagtgt cttgttaaca
     1741 gggggattta ccatgcttat tggctatgta cgcgtatcaa caaatgacca gaataccgat
     1801 ctacaacgta atgcgttgaa ctgtgcagga tgcgagctga tttttgaaga caagataagt
     1861 ggcacaaagt ccgaaaggcc ggggctgaaa aaactgctca ggacattatc ggcaggcgac
     1921 acactggttg tctggaagct ggatcggctg gggcgcagta tgcggcatct tgtcgtgctg
     1981 gtggaggagt tgcgcgaacg aggcatcaac tttcgtagtc tgactgattc aattgatacc
     2041 agcacaccaa tgggacgctt tttcttccat gtgatgggtg ccctggctga aatggaacgt
     2101 gaactgattg ttgaacgaac aaaagcagga ctggaagctg ctcgcgcaca gggacgaatt
     2161 ggcggtcgcc gtcccaaact ttcaccagaa taatgggcgc aggccgggcg actaattgca
     2221 tcaggagttt ctcgccagaa ggtggcgatc atctatgatg ttggcgaatc gacttttttg
     2281 tataagaggt ttccagccag ggttatataa aaccgcagac acggcgtata caagaacgtg
     2341 ctacggttga ctgaaaagtt aatgtggtat gtcacaggcc ttttgcgccg taaagccccg
     2401 tcctttaggg cgcggaggac gttaaagaaa gtggtgttct ttgtgttgct gaacttatgc
     2461 aaaatttttt tgcaccataa atccaacaac acattcaata acacattgct gtatcactat
     2521 gaaaataaat gtgtttttat ttgatttcac acatatcaca attgatgcat tttttagtga
     2581 ttaacaacgc cataaggaaa ctctcaaaac aacacaaacc gggcgggcat tgtacgcgca
     2641 ttctttactc agaccagttt tttcaccagc gcttcactat gacgaatacg ttctggtgcc
     2701 agttccagat cctgctgaat cagcgcaatg aacagcaaat ccgttaacat tccctgagcg
     2761 tgacaagcag agattgaagc actgtttgtc gcttgttctt cggcaatggt atacaggcaa
     2821 tgagaagcac gctgttgcag ggcattcgga gtaaagccgg taatcgccag cacttttccg
     2881 cccactcgca gcatctcatc tgccgccagg tttaactcgc gtcgtacacc ggtgtaggaa
     2941 atggctaata acagatcgtc aggggacgac gcctgtactg ttgcgagtag cgcatgcata
     3001 tcgcgcactg cggcagcatt gaagccaatc ttcatcagct tccaggcaaa gttttgcgcc
     3061 accagacccg aagcgccaat accggtcaga attatccgcc gcgcagagcg caacattgtt
     3121 acgcattcat gcagtttctc ttcactatta acgtttagcg ttgcgtacat agcggcagta
     3181 ttttctttaa tcagtttttc gccgaccagc cgtaacggat catcaccgcg gatttggtta
     3241 tgaatgggca cggagggcga ttccggctgg cttgccagcg cttcactcaa cgccaactta
     3301 agcgccggaa aacctttata gccgagtttt tgcgcgaact tcacgacgct ggactgactg
     3361 actccggctt cgttggccag ttgctgagag cttaaatggc gcgccgtatc aggttgtagc
     3421 agcagataat ccgccagttt tttatcactc tgggcaagcc cctggtaacg ctgacggata
     3481 cgaagtaagc cgttcatatc tttcctcgcg atgaaatagt ggcgacaaca ggcaatatga
     3541 gacacacgcg agtgaatttt atattccatt gccgctaatt gccatgaatt aaaaattcca
     3601 gttagccatg atgcggtcga ttgttgccgg aaaagtgatg atctatctgt taggctatgt
     3661 agtagcgaaa attaatcagg atgtttcagt ccagaggagt atggtttggc aactcacgag
     3721 cgtcgtgtgg tgttttttga cttagatgga acattgcatc agcaggatat gttcggcagt
     3781 tttctgcgct atttactgcg tcgccaaccg ctgaatgcgt tacttgtcct gccgttgtta
     3841 ccgattatag ccattgcgtt attgataaaa ggtcgtgcgg cacgctggcc gatgagtctg
     3901 cttctgtggg ggtgcacttt tggtcacagc gaagcacgtt tacagacgtt gcaggccgat
     3961 ttcgtgcgct ggtttcgcga caatgttacc gcctttccgc tggttcagga gcgattaacc
     4021 acctacctgt taagttccga tgctgatatc tggttgatta ccggctctcc gcagccgctg
     4081 gttgaagcgg tttatttcga tacgccctgg ctgccgcggg ttaatcttat cgccagccaa
     4141 attcagcgtg gctatggtgg ttgggtattg acgatgcgtt gtctgggaca tgaaaaggtc
     4201 gcacaactgg agcgcaaaat cggcactccg ctgcggctgt acagtggcta tagcgacagt
     4261 aatcaggaca atccgctgct ttatttctgt cagcatcgtt ggcgagtaac cccgcgcggt
     4321 gaactccagc aactggaata gagtaaagca tagcgtccgt gtataatgcg ccgcgctttt
     4381 ataaccggag ttttcttttt gtctgaagtc gaatttagcc acgaatactg gatgcgtcac
     4441 gcgctgacgc tggcgaaacg tgcctgggat gagcgggaag tgccggtcgg cgcggtatta
     4501 gtgcataaca atcgggtaat cggcgaaggc tggaaccgcc cgattggtcg ccatgatccc
     4561 accgcacatg cagaaatcat ggccctgcgg cagggtggtc tggtgatgca aaattatcgt
     4621 ctgatcgacg ccacgttgta tgtcacgctt gaaccatgtg taatgtgtgc cggagcgatg
     4681 atccacagtc gcattggtcg cgtggtcttt ggtgcgcgtg acgcgaaaac tggcgctgcg
     4741 ggatctttaa tggatgtgct gcatcatccg ggtatgaatc accgagtgga aattacggaa
     4801 ggaatactgg cggatgagtg cgcggcgttg ctcagtgact tctttcgcat gcgccgccag
     4861 gaaattaaag cgcagaaaaa agcgcaaccc tcgacggatt aattttgttt ctcttcactc
     4921 cctttcctgg aaaacagcag agagggagaa tgggtcaaat agtttgatga cgactgggaa
     4981 agaaacgaga gaaaaggaaa tttctctttg cccaactccg taggcgatac cgccggataa
     5041 tcctgcgcca gttgcatcgc cgcttctgta gcctgcttct ctttctcttg cagataaccc
     5101 accaggctaa tctgatactt acgaatattt tcgacataag cgtaggcttc atgtccacga
     5161 gcgtagccgt aagtcagctt gctgtaatag ggtttctggc taagtaaagg cagacgctgt
     5221 tttacgtcag cccaactgtc aggattccct ttggtttttg ccgtcagggc gcgggcatcc
     5281 agcatatgcg catagcccat attgtacgca gcgagggcaa accagatccg ctcgttctcc
     5341 ggcacacttt ccggcacttt actcatcata tcctgcaaat aacgcacgcc accgctgatg
     5401 ctctgttcgg catcggtacg atccgtaatg ccgaggcttt gcgcggtatt tttggttaac
     5461 atcatcatgc cgcgcacacc cgtcggtgaa gtggcctgtg catcccagtg cgattcctga
     5521 taagcaatag cggccagcaa acgccagtca atttcttcgg cgtatttctc aaacaggggc
     5581 tttaactgcg gcagtaccgc atcgacggcg cgtaaaaatg tgcgcgtatc gacgtaatca
     5641 aaatcatcgc catgccccag gtatttctct tcaatgcgtg ccagcgtaca gtcttcattc
     5701 atttcgttga agaagtcgag cagggcggcg gaaagggtat tatcgccatc taacgggcta
     5761 aaccaagtca ccggttgttc atcggtgata tcgagcgcta cggcgagctc cgggtgaacg
     5821 cgctgaaaca ggctgatggc gacagaatca gcaatggtgt aatcgagttt tccttcgatg
     5881 acatcttcca ttaattccgc agagcctttt ttgtcgtcta ccttccagct taattccggg
     5941 aattttgtgt ctttcagggt ttggagatcg ttaaccacca cgtgacccgg tgcaacggtg
     6001 agttgctccg ccgtcaggtt gcccagtgtg cgcgggcgat actgactcac tttataaacc
     6061 agttgttgtg acacggaata ataggtaggg ccaggctgat aattttttac ccgctcactg
     6121 ttatagacca gtcctgccgc cagcaggtcg gcgttaccat tatcgaggtc gtcaaacagc
     6181 tggctgatat tctgccgcac ggtcactttc agttttacac cgaggtaatc ggcaaactgt
     6241 ttcgccagtt cgtaatccag gccaaaaggt ttcccgttga tttcgttata agtcagggga
     6301 gtatgaatgg tgctcacacg caactctccc cgcgcttgaa tggcggcgat acggttgtcg
     6361 gctttaccaa accagggaat ggatggccag agagcgaccg cgagcagcag tgccagaatg
     6421 ccgatgaaca gataattaat ctttaatttt ttcaattagt taattctctg tgtcgtgcgc
     6481 gtcccagctt gaaaaaacgt aataatagtg aaaggtttac tcataaatga gcggcatttt
     6541 gcgtaaacct gcgccagatg gcaacttatt acagccattg gcggcacgcg ttgctaattc
     6601 acgatggtga ttttatttcc acgcaaacgg tttcgtcagc gcatcagatt ctttataatg
     6661 acgcccgttt cccccccttg ggtacaccga aagcttagaa gacgagagac ttatgatgga
     6721 aattctgcgt ggttcgcctg cactgtcggc attccgaatc aacaaactgc tggcacgttt
     6781 tcaggctgcc aggctcccgg ttcacaatat ttacgccgag tatgtccatt ttgctgacct
     6841 caatgcgccg ttaaacgatg atgagcacgc acaacttgaa cgcctgctga aatatggccc
     6901 ggcactcgcc agccacgccc cgcaaggcaa actcctgctg gtgaccccgc gtcctggcac
     6961 catctctccc tggtcttcga aagcgaccga tattgcccat aactgcgggc tacaacaggt
     7021 aaaccgcctt gagcgcggcg ttgcttacta tatagaagcc ggtacgctga ccaatgaaca
     7081 atggcagcag gttaccgctg aactgcacga ccgcatgatg gaaacggtct tttttgcttt
     7141 agatgatgca gagcagttgt ttgcccacca tcaaccgact ccggttacca gcgttgattt
     7201 gctggggcag ggccgtcagg cgctgatcga cgctaacctg cgtcttggcc tggctctggc
     7261 ggaagatgaa attgactatc tgcaggatgc tttcacaaag cttggtcgta acccgaacga
     7321 catcgagctg tatatgttcg ctcaggcgaa ctctgagcac tgccgccaca aaatttttaa
     7381 tgccgactgg attatcgacg gtgaacagca gccgaaatcg ctgttcaaga tgatcaaaaa
     7441 tactttcgaa accacgccag atcacgttct ctctgcttat aaagataacg ccgccgtaat
     7501 ggaaggttct gaagtgggcc gctactttgc tgaccacgaa acgggccgct acgatttcca
     7561 tcaggaaccg gcgcatattc tgatgaaagt cgaaactcac aaccacccga cggcgatttc
     7621 tccgtggccg ggcgcggcga cgggttccgg cggcgaaatt cgtgatgaag gcgccaccgg
     7681 acgcggtgcg aagccgaagg ctggtctggt tggtttctcc gtttccaacc tgcgaattcc
     7741 tggcttcgaa cagccgtggg aagaagattt cggtaagcct gagcgcattg tcaccgcgct
     7801 ggacatcatg accgaaggcc cgctgggcgg cgcggcgttt aacaacgaat ttggtcgtcc
     7861 ggcactgaat ggctacttcc gtacttatga agaaaaagtg aacagccaca acggcgaaga
     7921 gctgcgcggt tatcacaaac cgatcatgct ggcgggcggg atcggcaaca ttcgcgccga
     7981 tcacgtacaa aaaggcgaga tcaacgtcgg tgcgaagctg gtcgttctcg gcggcccggc
     8041 aatgaacatc ggtcttggcg gcggagcggc gtcttccatg gcgtcaggtc agtctgatgc
     8101 cgacctcgac tttgcttccg tacagcgcga caacccggag atggagcgtc gctgccagga
     8161 agtgatcgac cgttgctggc agcttggtga tgccaaccca atcctgttta tccacgacgt
     8221 cggcgctggc ggtctttcta acgcgatgcc ggaactggtg agcgacggcg ggcgcggcgg
     8281 taaatttgaa ctgcgcgata ttctaagcga cgaaccgggc atgagcccgc tggaaatctg
     8341 gtgtaacgaa tcccaggaac gctacgtgct ggcggttgct gccgatcaat taccgctgtt
     8401 tgacgaactg tgtaagcgtg agcgcgcacc ctacgcggtg attggtgaag cgaccgaaga
     8461 actgcatctt tctctgcacg atcgtcattt tgataatcag ccgatcgatc tgccgctgga
     8521 cgtcctgctt ggtaaaacgc cgaagatgac ccgcgatgta caaacgctga aagcgaaggg
     8581 cgatgcgctg gtccgtgaag ggatcaccat tgcagacgcg gtgaaacgcg tactgcatct
     8641 gccgaccgtg gcagagaaaa ccttcctggt gaccatcggc gaccgcagcg taaccggcat
     8701 ggtagcgcgc gatcagatgg tggggccgtg gcaggtgccg gtcgctaact gcgcggtcac
     8761 taccgccagc ctcgacagct actacggtga agcgatggcg attggcgagc gtgcgccggt
     8821 tgcactgctg gatttcgccg cctctgcccg tctggcggtc ggtgaagcgt taaccaatat
     8881 tgccgcaaca cagattggcg atatcaaacg cattaaactt tccgccaact ggatggcggc
     8941 ggcaggccac cctggtgaag atgcgggcct gtatgaagcc gttaaagccg tgggcgaaga
     9001 gctttgtccg gcgctgggcc tgacgatccc ggtgggtaaa gactctatgt cgatgaaaac
     9061 ccgctggcag gaaggtaacg aagagcgcga aatgacgtcg ccgctgtcgc tggtgatttc
     9121 tgcatttgcc cgcgtggaag atgtgcgtca caccatcacg ccacagcttt ctaccgaaga
     9181 taacacactg ctgctgattg atttgggcaa aggcaataac gcgctgggcg caacggcgct
     9241 ggcgcaggtt tatcgtcagc ttggcgacaa accggcagat gtacgcgacg tcgcgcaact
     9301 gaaaggcttc tatgacgcga ttcaggcgct ggttgcacag cgtaagctgc tcgcatacca
     9361 cgaccgctct gatggcggcc tgctggtaac gctggcggaa atggcctttg ctggtcattg
     9421 cggcattaac gcggatattg cctctctggg cgacgatcgc ctggcggcgt tgtttaacga
     9481 agaactgggt gcggtgattc aggttcgtgc cgctgaccgt gaagcggtcg agtccgtact
     9541 ggcacagcat gggcttgctg attgtgtcca ttatgtaggg caggcggttt ccggtgaccg
     9601 ttttgtgatt accgccaacg ggcagactgt attcagcgaa agccgcacca cgttgcgtgt
     9661 ctggtgggca gaaactacct ggcagatgca gcgcctgcgt gacaacccgg agtgtgctga
     9721 ccaggagcac caggcgaaat caaacgacgc cgatccgggc ctgaatgtga aactgtcgtt
     9781 cgatatcaac gaagatgtgg cagcaccgta tattgccact ggcgcacgtc cgaaagttgc
     9841 tgtgctgcgt gagcagggcg tgaactcgca tgttgaaatg gcggcagctt tccaccgtgc
     9901 aggctttgat gctatcgacg tgcatatgag tgacctgctg accggacgca cgggcctgga
     9961 agatttccac gccctggtcg cgtgcggtgg tttctcctac ggtgatgtgc tgggtgccgg
    10021 tgaaggttgg gcgaagtcaa tcctgttcaa tgaccgtgta cgcgatgagt ttgcaacctt
    10081 cttccaccgt ccgcaaacgc tggcgctggg ggtatgtaac ggttgccaga tgatgtctaa
    10141 cctgcgtgaa ctgatcccgg ggagcgagtt gtggccacgt tttgtgcgta acacctccga
    10201 tcgctttgaa gcgcgtttca gcctggttga agtaacccaa agcccgtctc tgctgttgca
    10261 ggggatggtg ggctcgcaaa tgccgattgc tgtctctcat ggtgaagggc gcgtggaagt
    10321 gcgtgatgcg gcgcatctgg cggcactgga aagcaaaggg ctggtggcac tgcgctatgt
    10381 cgataacttc ggcaaagtca ctgaaaccta cccggctaac ccgaacggtt caccgaacgg
    10441 tattacggcg gttacgactg aaagtggtcg agtcaccatt atgatgccgc acccggaacg
    10501 tgttttccgt actgtcagca actcctggca tccggaaaac tggggcgagg atggcccatg
    10561 gatgcgcatt ttccgcaatg cgcgtaagca gttggggtaa gtcatcagtt cattggtttt
    10621 gcagcccgga tgcggcatta agcaccaacc actatcataa gctcccaact ccgggagctt
    10681 ttttgtgtct gtaaatcacg acaatgggtg gtttaccgtg tcgctttctg gcgacactta
    10741 aatgattgat tttattgtaa tctaataatt ttatcttaag ttgtctcttt ttagcgacac
    10801 agtggctgat ttacattcaa atagcgaaga agccataaat ccctcaaagc aacgtataat
    10861 cagtacgtta ccaaactatt ttctttattg gcacagttac tgcataatag taagcagtgg
    10921 ctcattcacc gacttatgtc agccccttcg ggacgtgcta cataaaattc gaatgacgca
    10981 caacaaggtg cctgccgtcc aacttctgat atcagcgtag ctatatcaac catcgggcga
    11041 aacgtcgagt taggcaccgc cttattccat aacaaagccg ggtaattccc ggctttgttg
    11101 tatctgaact tcccctcggt tagcatcagg ctattcgcgt ctgacgagag taacaccttg
    11161 aaacgctggc ccgtttttcc ccgctcatta cgacaactgg taatgctggc atttttgcta
    11221 attctactac ccctgttggt gctggcatgg caagcctggc aaagcctgaa tgcgcttagc
    11281 gatcaggcgg cgctggttaa ccgcactacg cttattgatg cccggcgtag tgaagcgatg
    11341 accaacgcgg cgctggagat ggagcgtagc taccgtcagt attgcgtgct ggacgaccct
    11401 acgcttgcga aggtttatca aagccagcgc aagcgttaca gcgaaatgct cgatgcccac
    11461 gcaggcgtac tgccagacga taaactttac caggcattgc gtcaggattt gaacaatctg
    11521 gcccaacttc agtgtaacaa cagtggtccc gatgctgccg ccgccgcgcg tcttgaagcc
    11581 tttgccagtg ccaataccga aatggtgcag gccacgcgca cagtggtgtt ctctcgtggg
    11641 cagcaacttc agcgtgaaat cgccgaacgt gggcaatatt ttggttggca atcgctggtg
    11701 ctatttctgg tgagtctggt gatggtgcta cttttcacgc ggatgattat cgggccggtg
    11761 aaaaatatcg agcgcatgat caaccgtctg ggggaagggc gttctctggg caatagcgtt
    11821 tcgttcagtg gaccgagcga gttacgctcg gttgggcaac gtattctttg gttaagtgag
    11881 cgcctgtcat ggctggaatc ccaacgccat caatttttaa gacatttatc tcatgaatta
    11941 aaaacgccac tggcgagtat gcgcgagggc actgaattac tggctgacca ggttgtcggg
    12001 ccgcttacgc cagagcaaaa agaggtggtg agcattcttg atagcagcag ccgcaatttg
    12061 caaaaactga tcgaacaact gcttgattac aaccgtaaac aggcggacag tgcggtggaa
    12121 ctggagaatg ttgaattagc accgctggtg gagacagtgg tttctgctca tagcctgccc
    12181 gcacgggcta aaatgatgca taccgacgtc gatctcaaag caacagcttg cctggcggag
    12241 ccaatgctgc tgatgagcgt actggataat ctttactcca atgcggtgca ctacggggct
    12301 gaatccggta acatttgcct tcgcagcagt tcacatggtg cgcgggttta tattgatgtc
    12361 atcaatacag gcacgcccat tccgcaagag gaacgcgcca tgatcttcga accctttttt
    12421 cagggaagcc accagcgaaa aggggcggtg aagggcagcg gtctgggatt aagcattgcc
    12481 agggattgta ttcgccgtat gcaaggggaa ctgtatctgg tcgacgagag cgggcaagac
    12541 gtttgtttcc gcattgaatt accgtcgtcg aaaaacacga aataatgcta acagcctatc
    12601 ctatcaggct attttatttg tcagttcggc cccgggcagt gctcgaaatc ttcacgtacg
    12661 atatgtacgc tccggtttct ccgcgctgtc catgtccaga ctgtctgcaa caattacgcc
    12721 cactgggata ggctttaagt ctggtgaata tgcgacacat ttttcaacga ttattgcccc
    12781 gacggctgtg gctggcaggg ctgccatgtc tggcattgct gggttgcgtg cagaatcaca
    12841 ataagccagc cattgatacg ccagcagaag aaaaaattcc ggtttatcaa ctggctgact
    12901 atctttctac tgaatgtagc gacatctggg cgctgcaagg taaatcaacc gaaaccaatc
    12961 cgctttactg gctgcgggcg atggattgtg ctgatcgttt aatgcctgcg cagtcgcgtc
    13021 agcaagctcg ccaatatgac gacggcagct ggcaaaatac ctttaaacaa gggattttgc
    13081 tcgccgacgc caaaattacg ccgtatgagc gccgccagct ggtcgcgcgt attgaagcgt
    13141 taagtaccga gatcccggca caggttcgcc cactgtatca actctggcgc gacggtcagg
    13201 cgctgcaact gcaactggcg gaagagcgcc aacgttacag taaactccag cagtccagcg
    13261 acagcgagct ggatacattg cgccagcaac atcacgttct acagcagcaa ctggaactca
    13321 ccacccgcaa gctggaaaac ctgaccgata ttgaacgcca gctctcgacc cgcaaaccga
    13381 ctggaaattt ctcgccggat acgccgcacg agagtgaaaa gcccgcgcca tccacccatg
    13441 aggtcactcc tgatgagcca taaacctgcg catttattat tggtcgatga cgatccggga
    13501 ttgctgaaac tgcttggcct gcgcctgacc agcgaaggct acagtgtggt cacggcggaa
    13561 agtggcgctg aaggattacg ggtactgaat cgcgaaaaag tagatttagt tatcagcgac
    13621 ctgcggatgg atgaaatgga cggtatgcag ctatttgctg aaatccagaa agtgcagccg
    13681 ggaatgccag taattattct tactgcgcat ggttctatcc ccgatgccgt tgctgcaaca
    13741 cagcagggcg tttttagttt cctcaccaag cctgtcgata aagatgcgct atatcaggca
    13801 attgacgatg cgctggagca atccgcgcca gccaccgatg aacgctggcg cgaggcaatt
    13861 gtcacccgca gcccgctgat gctgcgtttg ctggaacagg cgcggctggt ggcgcaatca
    13921 gacgtcagcg ttttgattaa cggtcagagc ggcaccggga aagagatttt cgcccaggct
    13981 atccacaacg ccagcccgcg caacagcaaa ccatttattg ctattaactg tggcgcattg
    14041 cccgagcaat tgctggagtc ggagctgttt ggtcatgcgc gtggcgcgtt tactggcgct
    14101 gtcagcaatc gcgaaggttt attccaggcg gcggaaggcg gaacgctatt tctcgatgaa
    14161 attggcgata tgcctgcgcc gttacaggtc aaactgctgc gcgtgttgca ggagcgtaaa
    14221 gtgcgcccgc tgggcagtaa ccgcgatatt gatatcaatg tgcggattat ttctgccact
    14281 caccgtgatc tgccaaaagc gatggcgcgc ggggaattcc gcgaagatct ctattaccgc
    14341 ctcaacgttg tcagcctgaa aattccggca ctggcggagc gcacagaaga cattccgcta
    14401 ctggcaaatc acctgttgcg ccaggcggca gagcgacata aaccgtttgt ccgcgcgttc
    14461 tctaccgatg cgatgaaacg cctgatgacc gcgagctggc cgggtaatgt gcgccagttg
    14521 gtcaacgtga ttgaacagtg cgtggcgctg acctcatctc cggtgattag tgatgcgctg
    14581 gtggagcagg cgctggaggg tgaaaatacg gcgctgccaa cctttgttga ggcacgtaat
    14641 cagtttgaac tcaactattt gcgtaagctg ctgcaaatca ccaaaggcaa cgtcacccac
    14701 gcggcgagaa tggcggggcg caaccggaca gaattttata aactgctttc ccgacacgag
    14761 ctggatgcaa acgatttcaa ggaatgaatt ggcgttatgt gttacgttta gcagatcaaa
    14821 agacaggcga ccttttcaag gaatagcatg aaaaagattg atgcgattat aaaacccttc
    14881 aagctggacg atgtccgcga agcactggcc gaagtcggta ttaccggcat gacggtgacc
    14941 gaagtgaaag gctttggtcg ccagaaaggc cataccgagc tgtaccgcgg cgcggagtat
    15001 atggtggatt ttctgccgaa agtgaaaatt gagattgtcg taccggacga cattgtcgat
    15061 acctgtgtcg ataccattat tcgcacggcg caaaccggca aaatcggtga cggtaaaatc
    15121 ttcgtctttg acgtggcacg ggtcattcgc atccgtaccg gtgaagagga cgacgcggca
    15181 atttaaaccg cgtcaagggt tgccggatgt ttccatccgg caacatcaaa ttacagcacc
    15241 ttatgcgggc caaagcattc gtaatgaatg ttttcctgct tcacgcccag atccactaac
    15301 tgtttcgcgg taaactgcat gaagccaacc gggccgcaga gatagaactg cattgtcgga
    15361 tcgctgaacg caccttccag tttgctcaaa tccatcagac cttcgctatc aaactgacct
    15421 ttagcgcgat cggcttcgct cggctgacga tgccaggtgt gcgcggtaaa gcgcggcagt
    15481 gactgcccca gttccttaac ttcatcggca aaggcgtgaa catcgccatt ttctgccgca
    15541 tggaaccagt tcacttgtgc tgtgtggcct gcttttgcca gcgtgtcgag cattgccagc
    15601 attggcgttt gaccaacacc ggcagagatt aacgtcactg gtgtgtcatc tgcgacagcc
    15661 ataaagaaat cacctgccgg agcgaccagt ttcacgacat cgccaacatt ggcgtgattg
    15721 tgcaaccagt tggatacctg cccaccctct tcgcgtttca ccgcaatacg atagcctttg
    15781 ccatccggtt tgcgagtcaa agagtactga cgaatttcct gatgcgggaa accttccggc
    15841 ttcagccaga cgccgagata ttgccccgga cggtattctg ccactgcgcc accgtcgacc
    15901 ggctccagtt cgaagctggt gataagcgcg ctgcgcggtg ttttagccac aatgcggaaa
    15961 tcgcgagtac cttcccaacc accggctttg ctggcgtttt cgttatagat ttccgcctcg
    16021 cgattgataa atacattagc cagtacacca taggctttac cccacgcgtc cagcacttcc
    16081 tgccccgggc tgaacatttc gtccagcgtt gccaacaggt gttcaccgac gatgttgtac
    16141 tgttccggtt tgatctggaa gctggtgtgc ttctgcgcga ttttttctac cgctggcagc
    16201 agcgcaggca ggttttcaat attactggcg taggcggcaa tagcgttaaa cagggcttca
    16261 cgttgatcgc cattacgctg gttactcatg ttaaaaattt ctttgagttc tgggttatga
    16321 gtaaacatac ggtcgtagaa atgggcggtt aactttggcc ccgtttccac cagtaaaggg
    16381 atggtggctt ttactgtagc gatggtttga gcgtcaagca tatggtcttc ctttttttgc
    16441 atcttaattg atgtatctca aatgcatctt ataaaaaata gccctgcaat gtaaatggtt
    16501 ctttggtgtt tttcagaaag aatgtgatga agtgaaaaat ttgcatcaca aacctgaaaa
    16561 gaaatccgtt tccggttgca agctctttat tctccaaagc cttgcgtagc ctgaaggtaa
    16621 tcgtttgcgt aaattccttt gtcaagacct gttatcgcac aatgattcgg ttatactgtt
    16681 cgccgttgtc caacaggacc gcctataaag gccaaaaatt ttattgttag ctgagtcagg
    16741 agatgcggat gttaaagcgt gaaatgaaca ttgccgatta tgatgccgaa ctgtggcagg
    16801 ctatggagca ggaaaaagta cgtcaggaag agcacatcga actgatcgcc tccgaaaact
    16861 acaccagccc gcgcgtaatg caggcgcagg gttctcagct gaccaacaaa tatgctgaag
    16921 gttatccggg caaacgctac tacggcggtt gcgagtatgt tgatatcgtt gaacaactgg
    16981 cgatcgatcg tgcgaaagaa ctgttcggcg ctgactacgc taacgtccag ccgcactccg
    17041 gctcccaggc taactttgcg gtctacaccg cgctgctgga accaggtgat accgttctgg
    17101 gtatgaacct ggcgcatggc ggtcacctga ctcacggttc tccggttaac ttctccggta
    17161 aactgtacaa catcgttcct tacggtatcg atgctaccgg tcatatcgac tacgccgatc
    17221 tggaaaaaca agccaaagaa cacaagccga aaatgattat cggtggtttc tctgcatatt
    17281 ccggcgtggt tgactgggcg aaaatgcgtg aaatcgctga cagcatcggt gcttacctgt
    17341 tcgttgatat ggcgcacgtt gcgggcctgg ttgctgctgg cgtctacccg aacccggttc
    17401 ctcatgctca cgttgttact accaccactc acaaaaccct ggcgggtccg cgcggcggcc
    17461 tgatcctggc gaaaggtggt agcgaagagc tgtacaaaaa actgaactct gccgttttcc
    17521 ctggtggtca gggcggtccg ttgatgcacg taatcgccgg taaagcggtt gctctgaaag
    17581 aagcgatgga gcctgagttc aaaacttacc agcagcaggt cgcgaaaaac gctaaagcga
    17641 tggtagaagt gttccttgag cgcggctaca aagtggtttc cggcggcact gataaccacc
    17701 tgttcctggt tgatctggtt gataaaaacc tgaccggtaa agaagcagac gccgctctgg
    17761 gccgtgctaa catcaccgtc aacaaaaaca gcgtaccgaa cgatccgaag agcccgtttg
    17821 tgacctccgg tattcgcgtg ggtactccgg cgattacgcg tcgcggcttc aaagaagcag
    17881 aagcgaaaga actggctggc tggatgtgtg acgtgctgga cagcatcaat gatgaagccg
    17941 ttatcgagcg catcaaaggt aaagttctcg acatctgcgc acgtttcccg gtttacgcat
    18001 aagcgaaacg gtgatttgct gtcaatgtgc tcgttgttca tgccggatgc ggcgtgaacg
    18061 ccttatccgg cctacaaaac tttgcaaatt caatatattg caatatccgt gtaggcctga
    18121 taagcgtagc gcatcaggca atcttaggta tgtgatcatc aaggcttcct tcgggaagcc
    18181 tttctacgtt attgcgccat caaatctgtc gtaactgcgc ctcaacatac aaatagccaa
    18241 tccccaacac ctgttgtgcg cggcttaatt gcccaaagcc aatttgcgtt gctttcacgc
    18301 tcggcccttc gtcgcggtcg aacgggttaa atcccgtctg gcgaataatg gtattaagcc
    18361 agtgctcacc aaaggcacaa ctgcgaccgt acaaccaaat ctgattaata ttgaggatgt
    18421 tgaggaagtt atacagactc aaaccaatgg cattggcact gcgatcaacc caactggtga
    18481 tccacggttc tccactctac caggcagcga ttaactgcgc tgtagtcagt ttttccggat
    18541 caagctgagt attaaccggt tgtgatttta gccatacccg cgcctgtttt tttaatgcac
    18601 ttaacgaggc gacggtttcc aggcagccgt aacgtccaca gtcgcagacg acgccatccg
    18661 gattaacgat ggtgtgacca atctgtccgc taccatacaa actgccgcga taaatttgct
    18721 cgttaatcac gaacgacgag ccaatgccgt aatcaacgtt gatcacgcag aaatcccgtt
    18781 tctgcgaatt attttgccat ttctccgcca gcgccagcat cacgcagtca ttatcgacca
    18841 tcacccgaat gccgagcttc tcttccagca gatacttcac ctcaaccggc gttgtccacg
    18901 gcgcttgcgg catggtttgc gacacgccgg tcaccggatc aacctgaccg tggattgcca
    18961 gcgccagatt gatggtgcgg tccggccaca atttacggtg gcgatgccag catttttcga
    19021 tttcggacag cagcgcctgc ggcgtcggtg catcaatctg taaatactca aattcacctt
    19081 tcggacttaa acaagcatta gcaacctgac actcaatact ggtgggcgtc acgttcaggc
    19141 acagcgtcca gtcaccttcc ggcgcaatca gccatgtacc gctactatgc ccgcgcgtct
    19201 ggctttcatc gtcgatattc accacccgtt tttcgctttc cagttcctgc aaaatattac
    19261 tgactgccgg aatcgaaatt tgcgccagcc gggccagcgt tgatttattg gcgcgctttt
    19321 gccggtacag cagttccaga atcacgcatt tgttatggtg gcgaatctgt tgattgttaa
    19381 tgcaggctct catcttcact aaaccttgtt tagttaattg cgtgattatt ccgcgaaaac
    19441 attgccagat tgttcaatac actgccacaa atcttttaat tcagtatgtc ttgttaatat
    19501 tgagggcacc atgactccag taaaagtgtg gcaagagcgc gttgagatcc cgacctatga
    19561 aaccgggccg caggatatac atcccatgtt cctggaaaat cgcgtttatc agggatcgtc
    19621 cggcgcggtt tatccctacg gcgtgaccga tacgctgagc gagcagaaaa ccctgaaatc
    19681 ctggcaggcg gtgtggctgg aaaacgacta catcaaagtg atgatcctgc cggaactggg
    19741 cggtcgggtg catcgcgcgt gggataaagt gaaacagcgc gattttgttt atcacaatga
    19801 agtcattaaa cctgcgctgg tggggctgct ggggccgtgg atttccggtg ggattgagtt
    19861 taactggccg caacatcatc gcccgaccac ctttatgccc gttgatttca ccctcgaagc
    19921 ccatgaagac ggtgcacaga cggtgtgggt aggcgaaacg gagccgatgc atggtttaca
    19981 ggtgatgaca ggtttcaccc tgcgccctgg gcggcgctgg aaatcgccag ccgcgtctat
    20041 aacggcaacg ccacgccgcg tcatttcttg tggtgggcca acccggcagt gaaagggggt
    20101 gaagggcatc agagcgtttt cccaccggat ttgactgcgg tgtttgatca cggcaaacgg
    20161 gccgtctccg ctttccccat cgctaccggc acttactaca aagtggacta ctccgctgga
    20221 gtggacattt ctcgctataa aaatgtgccc gttccaacct catatatggc cgaaaaatcg
    20281 cagtacgatt ttgtcggcgc gtggtgtcac gatgaagatg gcggtttgct gcacgttgcc
    20341 aaccaccata ttgcgctagg taaaaaacag tggagttggg gacacagtga atttggccag
    20401 gcgtgggata agagtctgac cgacaataac ggcccgtata tcgaactgat gaccggtatt
    20461 tttgccgata accagcccga ttttacctgg cttgatgctt acgaagagaa gcgttttgag
    20521 cagtatttcc tgccttatca ttctctgggc atggtgcaaa atgcctcccg cgatgcggtg
    20581 ataaaactcc agcgtagtga gcaggggatt gagtgggggc tgtatgccat ctctccgttg
    20641 aacggatacc gcctggcgat ccacgaaatc ggcaaatgca acgcgttact tgatgatgcc
    20701 gtggcactga tgcctgcgac cgccatccag ggcgtgttgc acggtatcaa tcctgatagg
    20761 ctgaccattg agctttccga tgccgacggc aatattgtac tgagttatca ggaacatcag
    20821 ccgcaagcgt tgccgttacc ggacgtcgcc aaagcgccac tggcagcaca agacattacc
    20881 agtacagatg aagcctggtt tatcggtcag catctggagc aatatcatca cgccagccgt
    20941 tcaccgttcg attactacct gcgcggcgtg gcgctggacc cgctggatta tcgctgtaac
    21001 ctggcgctgg cgatgctgga atataaccgc gcagatttcc cgcaagcggt ggcgtatgcc
    21061 agtcaggcgc tgaaacgcgc acatgcgctg aacaaaaacc cgcagtgtgg acaagcaagt
    21121 ttgattcgcg ccagtgctta cgaacgtcag ggacaatatc aacaagccga agaggatttc
    21181 tggcgtgcgg tctggagcgg caacagcaaa gccggtggct attatggcct ggcacgactg
    21241 gcggcgcgta atggtaactt cgacgctggt ctggattttt gccaacaaag tcttcgcgcc
    21301 tgcccaacca atcaggaagt gctttgcctg cataatctgc tgctggtgtt aagtggtcgt
    21361 caggacaacg cgcgtttgca gcgcgcgaaa ctgctgcgtg attatccgct gaacgccatt
    21421 ctgtggtggc tgaactggtt cgatggtcgt agcgaatcag ccctcgcgca gtggcgcggt
    21481 ctgtgtcagg gacgcgacgt taacgccctg atgaccgccg ggcaactgat taactgggga
    21541 atgcccactc tggcgacaga gatgctgaac gcactggact gtcagcgcac gttatcgctt
    21601 tatctacaag ccagcttgct gccgaaagcc gaacgcggcg aactggtcgc aaaagccatt
    21661 gatgtcttcc cgcagtttgt ccgtttcccg aatacgctgg aagaagtggc ggcgctggag
    21721 agtattgaag agtgctggtt tgcccgtcat ttactggcct gcttctacta caacaagcgc
    21781 agctacaaca aagccattac tttatggcaa cgttgcgtag agatgtcgcc ggagtttgcc
    21841 gatggctggc gcgggttggc gatccatgcg tggaataagc aacacgatta tgaactggcc
    21901 gcgcgttatc ttgataatgc ttatcagctt gcgccgcagg atgcacgtct gcttttcgaa
    21961 cgggatttgc tggataagtt aagtggagcc acaccggaga aacgactggc gcgtctggaa
    22021 aataatctgg aaattgcgct gaaacgcgac gacatgaccg cagaactgct caatttgtgg
    22081 catctcacgg gtcaggcaga caaagcggcg gacattctcg ccacgcgcaa attccacccg
    22141 tgggaaggcg gggaagggaa ggtcaccagt cagtttatcc tcaaccagtt attacgcgcc
    22201 tggcagcatc ttgatgccag acagccgcag caggcctgcg aactgcttca tgccgcgctg
    22261 cattatccgg agaatttaag cgaaggccgt ttaccggggc aaactgataa cgacatctgg
    22321 ttctggcagg cgatatgcgc caacgcgcag ggcgatgaaa ctgaagcgac gcgttgttta
    22381 cgtctggcgg cgaccggcga tcgcaccatt aacatccaca gttattacaa cgatcagccg
    22441 gttgattatc tcttctggca aggaatggcg ctgcgactgc tgggcgaaca acaaatcgca
    22501 cagcaactgt ttagtgaaat gaaacagtgg gcgcaagaga tggcgaaaac cagtattgag
    22561 gcggatttct ttgctgtttc acaacctgac ctgttgtcgc tgtatggcga tttacaacag
    22621 cagcataaag aaaaatgcct gatggtggcg atgctggcgt ccgcgggact cggggaggtt
    22681 gcgcaatatg aatctgctcg cgctgaattg acggcgatta atccggcctg gccgaaagcg
    22741 gcattattca ccaccgtgat gccttttatt tttaactacg ttcactaaac cctattaact
    22801 gtaatatttg agcggcacgc gctgccgctc atcacaaaat aattagaata agccgttgtt
    22861 aataaaaact ctacaggagg cactatgcct aaaaaaatga gaacaacccg taatctatta
    22921 ttaatggcta cgctattagg tagcgcacta tttgccaggg ctgcggataa agaaatgacc
    22981 attggtgcaa tataccttga taccctggga tattacgctg gagtgcgtca gggcgttcag
    23041 gatgcggcaa aagattcttc agtacaggta cagttaattg aaactaacgc ccagggtgat
    23101 atttcgaaag aaagtacctt tgttgatacc ctcgtggcgc gtaatgtcga tgccattatt
    23161 ttatcggcag tgtctgaaaa tggcagtagc cgtaccgttc gtcgcgccag tgaagcgggt
    23221 attccggtga tttgctacaa cacctgtatt aatcaaaagg gtgtcgataa atatgtctcg
    23281 gcgtatctgg tcggcgatcc actggaattt ggtaaaaaac tgggtaacgc tgccgccgat
    23341 tattttattg ccaataaaat tgaccagccg aaaattgccg tcatcaattg cgaagccttt
    23401 gaagtttgtg tgcagcgacg taaaggattt gaagaagtat taaaatcccg cgttcccggc
    23461 gcgcaaattg tcgctaatca ggaagggact gttttaaata aagcgatttc cgttggtgaa
    23521 aaactgatta tctccacgcc ggatctcaac gccattatgg gggagtcggg cggtgcgaca
    23581 ctcggcgcgg taaaagcggt acgtaatcaa aatcaggccg gaaaaattgc tgttttcggt
    23641 tcggatatga caaccgaaat tgctcaggag ctggaaaaca atcaggtgct gaaagcggta
    23701 gtggatattt ccggtaagaa aatgggcaat gctgttttcg cgcaaacatt gaaggttatc
    23761 aataaacaag ccgacggtga aaaagtgatt caggtgccaa tcgatctcta taccaaaacg
    23821 gaagatggta aacagtggct ggcaacgcac gttgatggtc tgccctaatc gctgattcgc
    23881 agaggtccct atgttcacgg caacagaggc agtcccggta gcaaaagtgg tggcaggaaa
    23941 taagcgttac cccggcgtcg ttgcgctgga taacgttaac ttcacgctca ataaaggcga
    24001 agttcgtgcg ctgttaggta aaaacggcgc gggcaaatcg actctcattc gaatgcttac
    24061 cggcagcgaa cgcccggata gcggtgatat ctggattggc gagacgcgac tggaaggtga
    24121 cgaagctacg ttgactcgcc gtgccgctga actgggggtt cgtgcggttt atcaggaatt
    24181 aagtctggtg gaagggctga cggtggcaga aaacctctgc cttggtcaat ggcctcgccg
    24241 caacggcatg attgattacc tgcaaatggc gcaggatgcc caacgttgct tacaggcgct
    24301 gggcgttgac gttagtcctg aacaacttgt ttcaacgcta agcccggcgc aaaagcaact
    24361 ggtggaaatt gcacgggtga tgaagggcga gccgcgcgtg gtcattcttg atgaacccac
    24421 cagctcgctt gccagtgcag aagttgaact ggtgatcagt gcggtgaaaa agatgtcggc
    24481 actgggcgtg gcggtgattt atgtcagcca ccggatggaa gaaatccgcc gcattgcctc
    24541 ctgtgccacc gttatgcgcg atggtcaggt ggcgggcgat gtgatgctcg aaaacacctc
    24601 cacgcatcat attgtgtcgc taatgctagg gcgtgatcac gttgatattg cgccggttgc
    24661 ccctcaggaa attgtggatc aggccgtact ggaagtccgt gcgttacgcc ataagcccaa
    24721 gctggaggat atcagcttta cgttacgtcg cggcgaagtg cttggcattg ctggcctgct
    24781 gggggcaggg cgcagtgaat tgctgaaggc gattgttggg ctggagacgt atgaacaggg
    24841 cgaaattgtt atcaacggcg agaaaatcac gcgccccgat tacggcgaca tgctgaaacg
    24901 cggcattggc tatacgccag aaaaccgcaa agaagcgggg atcattccct ggttgggcgt
    24961 tgacgaaaat acagtgctga ccaatcggca aaaaatcagc gccaacggag tgctgcaatg
    25021 gtccaccatc cgccgcctga ccgaagaggt gatgcagcgg atgacggtca aggccgctag
    25081 tagcgaaacg cccatcggca cgctttctgg tggcaatcag caaaaagtgg tgatcggtcg
    25141 ttgggtctat gccgccagcc agattttgtt gctcgacgag ccaacgcgcg gcgtcgatat
    25201 cgaagccaaa cagcagattt accgtattgt ccgtgagctg gctgccgaag gaaaaagcgt
    25261 ggtgtttatc tccagtgaag tggaggagtt gccgctggtg tgtgaccgca tcctgttatt
    25321 acagcacggc acgttctcgc aggagtttca ctcgccggtc aatgtggatg agctgatgtc
    25381 cgccattctg tctgtgcact gatgaatttt accgaggaac ctgaagatgt ctgcttcgtc
    25441 attaccattg ccgcagggca agagcgtctc gctcaaacaa tttgtcagtc gccatattaa
    25501 tgagatcggt ttgctggtgg tgattgccat tctttatctg gtcttctccc tgaacgcgcc
    25561 tggctttatc tcattgaata accagatgaa cgtgctgcgc gatgccgcca ccattgggat
    25621 tgccgcctgg gcgatgacgc tgattattat ctccggtgaa attgatgtca gcgttgggcc
    25681 gatggtggct tttgtctcgg tgtgcctggc atttttgctg caatttgaag ttccgttggc
    25741 gattgcttgt ctgctggtgt tgctgttagg cgcgctgatg ggaacgctcg ccggggtgct
    25801 gcgcggcgtg tttaacgtac caagtttcgt tgccacactg gggctgtgga gcgccctgcg
    25861 cggaatgggg ctgtttatga ccaacgcact gccagtgccg attgacgaaa acgaggtgct
    25921 ggactggctg ggcggacaat ttctcggtgt gccagtatcc gcgctgatca tgatggtgtt
    25981 gtttgcgctg tttgtgttca ttagccgaaa aaccgccttc gggcgctcgg tttttgccgt
    26041 tggcggtaat gccacggcgg cgcagttgtg cggtattaac gttcgtcggg tgcgcattct
    26101 tatctttacc ctttcgggat tattagcggc ggtgaccggc attttgctgg cggcacgcct
    26161 cggttccggt aacgcaggtg ccgcaaacgg tctggagttt gacgtcatcg ccgcggtcgt
    26221 ggtcggcggt acggcacttt ccggcggtcg cggttccctg ttcggtacat tgcttggtgt
    26281 gctggtgatt acgctaatcg gtaacggtct ggtgctgctc ggtattaact cctttttcca
    26341 gcaggtggta cgcggcgtca tcatcgtggt ggcggtgctg gcgaatatct tgctgaccca
    26401 gcgaagcagt aaagcgaaac gctaaacctt aacgcccctg gcgcgggtgc ggcagggatg
    26461 aaaattaatc gtattgttaa tgagggattt atgaaaacga tgctggcagc ttatttacca
    26521 ggaaattcaa ccgtcgatct gcgggaagtt gcggtgccga cgccggggat taaccaggta
    26581 ctgatcaaaa tgaaatcctc cgggatttgc ggaagcgatg tccactatat ctatcatcaa
    26641 cactgtgcca cggcggcggc acccgataaa ccgttatacc agggatttat caacggtcat
    26701 gaaccgtgcg ggcagattgt ggcgatgggg caaggctgcc gccattttaa agagggcgac
    26761 cgcgtgctgg tgtatcacat ttctggctgt ggtttttgcc cgaactgccg ccgcggcttt
    26821 cctatctctt gtactggcaa aggaaaagcg gcttacggct ggcagcgtga cggcggtcat
    26881 gccgaatatc tgctggcgga agaaaaagat ctgatcctcc tgccggatgc gctgagctac
    26941 gaagatggtg cgtttatcag ttgcggcgtt ggcacggcct atgaagggat tttgcgcggc
    27001 gaagtttccg gcagcgataa cgtgctggtg gtcggtctgg ggccggtcgg catgatggcg
    27061 atgatgctgg cgaaaggtcg cggtgcaaaa cggatcatcg gcgttgatat gctgccggaa
    27121 cgtctggcga tggcaaaaca gttaggggtg atggatcacg gctatttagc aaccaccgaa
    27181 ggtctgccgc agattatcgc cgaactcacc cacggtggcg cggatgttgc gctcgattgt
    27241 tctggtaatg ccgcaggtcg cttgctggca ctgcaatcca ccgctgactg gggacgggtg
    27301 gtttacattg gtgaaaccgg aaaagtggaa ttcgaggtca gcgccgatct gatgcaccat
    27361 caacggcgga ttatcggctc ctgggtgacc agtctgttcc atatggaaaa atgcgcccac
    27421 gatttgactg actggaagct gtggccgcgt aacgccatta cccatcgctt ctcgctggaa
    27481 caggcaggtg atgcctatgc gctgatggcg agcggcaaat gcgggaaagt tgtgattaac
    27541 ttcccggatt aaggagttag cggatgacca tctatacctt atcccacggg tcgctaaagc
    27601 tggacgtttc tgaccagggc ggcgtaatcg aaggcttctg gcgcgatacg acgccgctct
    27661 tacgtcctgg taaaaaaagc ggtgtggcaa ccgatgcctc atgttttccg ctggtgccat
    27721 ttgcaaaccg ggtgagcggc aaccgttttg tctggcaggg gcgtgagtat caactgcaac
    27781 cgaatgttga gtgggatgcg cactatctac acggcgatgg ctggctgggc gagtggcaat
    27841 gtgtctcgca cagcgatgat agtttgtgtc tggtgtatga acatcgcagc ggtgtctatc
    27901 actatcgggt aagtcaggcg tttcatttaa cggcggatac gctgacggtg acgctctctg
    27961 tcaccaatca aggggcagag acgctgccat ttggtaccgg ctggcatcct tattttccgt
    28021 tgtcaccaca aacgcggatt caggcgcagg cgagcggtta ctggctggaa cgggagcagt
    28081 ggctggcggg tgagttttgc gagcagctac cgcaggaact ggattttaac cagctcgcgc
    28141 cgttgccgcg ccagtgggtg aacaatggtt ttgccggatg gaatggtcag gcccgcatcg
    28201 agcagccgca ggaaggttat gccatcatca tggaaacgac gccacctgca ccgtgttatt
    28261 tcatctttgt ttccgaccct gcgtttgata aaggatatgc gtttgatttc ttctgtctgg
    28321 aaccgatgag ccatgcgccg gatgatcatc atcgcccgga agggggtgat ctcattgcgc
    28381 tagcaccagg ggaatcaaca acttcagaga tgtcgttgcg ggtagagtgg ctgtaattta
    28441 ttaaatgccg gataaggcgt tcacgccgca tccggcatct ttcctaacgc cgatcgagag
    28501 aaattgcccc cggcccggta attgccaata gcaagaacgc gccagcgata ctgacattct
    28561 tccagaaatt aatcatattt ggcccaaccg catcgccggt catatcccag taatggtgac
    28621 caatcaccgc cgtacccagc gtgtagaaaa taaacagcac cgccagcgga cgggtgaaaa
    28681 agccaagcac gattaatatc gcggcgggca cttccataac taccgcaata atcgctgcca
    28741 gcatcggcat tggcgcgccc aacgaggcca tatattggac cgtaccgtca aagcccatca
    28801 ttttgggaaa accaaaaata atgaaaatta agaccacggc gatacgggca attaataaca
    28861 aaacggggcg ggcagctcca aaatcaaaat aacg
//
LOCUS       DAZPDU010000038        28350 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZPDU010000038 DAZPDU010000000
VERSION     DAZPDU010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28350
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.038"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..469
                     /locus_tag="ACHURS_001511"
     CDS             <1..469
                     /locus_tag="ACHURS_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1237281.1"
                     /translation="SGTSVTGSTVKMGEYSAEQIRNGATPVPFDISLQNCVRVTNIET
                     KLVSTKVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSNSVYKDYD
                     PRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVTYP"
     gene            582..1376
                     /gene="panB"
                     /locus_tag="ACHURS_001512"
     CDS             582..1376
                     /gene="panB"
                     /locus_tag="ACHURS_001512"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFR1237282.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTASVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPIELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1388..2239
                     /gene="panC"
                     /locus_tag="ACHURS_001513"
     CDS             1388..2239
                     /gene="panC"
                     /locus_tag="ACHURS_001513"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFR1237283.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKIVELA"
     gene            complement(2531..3427)
                     /gene="rpnC"
                     /locus_tag="ACHURS_001514"
     CDS             complement(2531..3427)
                     /gene="rpnC"
                     /locus_tag="ACHURS_001514"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFR1237284.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMVFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFAQRFLSKG
                     MSREDVAEMTNLSLAEIDRLIN"
     gene            3701..4081
                     /gene="panD"
                     /locus_tag="ACHURS_001515"
     CDS             3701..4081
                     /gene="panD"
                     /locus_tag="ACHURS_001515"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFR1237285.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(4085..5314)
                     /locus_tag="ACHURS_001516"
     CDS             complement(4085..5314)
                     /locus_tag="ACHURS_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFR1237286.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQVRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(5378..5818)
                     /locus_tag="ACHURS_001517"
     CDS             complement(5378..5818)
                     /locus_tag="ACHURS_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFR1237287.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5923..6693)
                     /locus_tag="ACHURS_001518"
     CDS             complement(5923..6693)
                     /locus_tag="ACHURS_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1237288.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6690..7616)
                     /locus_tag="ACHURS_001519"
     CDS             complement(6690..7616)
                     /locus_tag="ACHURS_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1237289.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7725..8387
                     /gene="can"
                     /locus_tag="ACHURS_001520"
     CDS             7725..8387
                     /gene="can"
                     /locus_tag="ACHURS_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFR1237290.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8428..8964)
                     /gene="hpt"
                     /locus_tag="ACHURS_001521"
     CDS             complement(8428..8964)
                     /gene="hpt"
                     /locus_tag="ACHURS_001521"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFR1237291.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILRDE"
     gene            9170..11560
                     /gene="gcd"
                     /locus_tag="ACHURS_001522"
     CDS             9170..11560
                     /gene="gcd"
                     /locus_tag="ACHURS_001522"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFR1237292.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWCSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKINEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11607..13157)
                     /gene="cueO"
                     /locus_tag="ACHURS_001523"
     CDS             complement(11607..13157)
                     /gene="cueO"
                     /locus_tag="ACHURS_001523"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFR1237293.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEIPGDVDGGPQGIIPPGGKRTVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGM
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            13323..13670
                     /locus_tag="ACHURS_001524"
     CDS             13323..13670
                     /locus_tag="ACHURS_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFR1237294.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13776..14642
                     /gene="speE"
                     /locus_tag="ACHURS_001525"
     CDS             13776..14642
                     /gene="speE"
                     /locus_tag="ACHURS_001525"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFR1237295.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGIVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSYRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14658..15452
                     /gene="speD"
                     /locus_tag="ACHURS_001526"
     CDS             14658..15452
                     /gene="speD"
                     /locus_tag="ACHURS_001526"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFR1237296.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15490..15852)
                     /gene="yacL"
                     /locus_tag="ACHURS_001527"
     CDS             complement(15490..15852)
                     /gene="yacL"
                     /locus_tag="ACHURS_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFR1237297.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(16027..18624)
                     /gene="acnB"
                     /locus_tag="ACHURS_001528"
     CDS             complement(16027..18624)
                     /gene="acnB"
                     /locus_tag="ACHURS_001528"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFR1237298.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18979..20736
                     /locus_tag="ACHURS_001529"
     CDS             18979..20736
                     /locus_tag="ACHURS_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFR1237299.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVETQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQNQQRREAARERIQSATPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRIQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNE
                     QRSAVRERLSERGARRQER"
     gene            complement(20807..22231)
                     /gene="lpdA"
                     /locus_tag="ACHURS_001530"
     CDS             complement(20807..22231)
                     /gene="lpdA"
                     /locus_tag="ACHURS_001530"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFR1237300.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22556..24448)
                     /gene="aceF"
                     /locus_tag="ACHURS_001531"
     CDS             complement(22556..24448)
                     /gene="aceF"
                     /locus_tag="ACHURS_001531"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFR1237301.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPASAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKLTPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(24463..27126)
                     /gene="aceE"
                     /locus_tag="ACHURS_001532"
     CDS             complement(24463..27126)
                     /gene="aceE"
                     /locus_tag="ACHURS_001532"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFR1237302.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYIPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27287..28051)
                     /gene="pdhR"
                     /locus_tag="ACHURS_001533"
     CDS             complement(27287..28051)
                     /gene="pdhR"
                     /locus_tag="ACHURS_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFR1237303.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
BASE COUNT         6854 a         7695 c         6952 g         6849 t
ORIGIN      
        1 tagtggaact tctgtgactg gttctacggt aaaaatgggt gaatatagtg cagaacaaat
       61 cagaaacggt gccacaccgg ttccttttga tatttcactt caaaactgcg ttcgtgtgac
      121 taatattgag acaaaattag tttcaacaaa ggttggtact gaaaacgggc aactccttgg
      181 taatactctc acaggtaatg acgcagcaaa aggagtcggt gtactcatag aaggtttagc
      241 aactagtaaa aatcctctaa tgacattgaa acctaatgat tcaaattctg tttataaaga
      301 ttacgacccg agaggcaaag acgatacaac aggaggagtt tacccggatc aagatacagg
      361 tataacatac cctctccatt tccaggccac gctacaacag gatggaacta taccaataga
      421 agctggtgag tttaaagcca ccagtacttt ccaggtaacc tacccttaat aagtccaccg
      481 caccgccatc atctggcggt gcggtaattg ataaatcatt gcccccacca atgtgactga
      541 taaaatgcca ggcaacacga catcatttat caggacacgt tatgaaaccg accaccattg
      601 cttcacttca gaaatgtaag caggataaaa agcgtttcgc gaccatcacc gcttacgact
      661 acagcttcgc gaaactattt gctgatgaag gacttaacgt catgctggta ggcgactcgc
      721 tgggcatgac ggttcagggt cacgactcca ccctgccggt gaccgttgcc gatatcgcct
      781 accacactgc ctccgtacgt cgtggtgcgc caaactgcct gctgttggct gacctgccgt
      841 ttatggcgta tgccacgccg gaacaagcct ttgaaaacgc cgcaacggtt atgcgtgccg
      901 gtgccaatat ggtcaaaatt gagggcggtg agtggctggt cgaaaccgta aaaatgctga
      961 ccgaacgtgc cgttcctgta tgtggtcact taggtttaac cccacagtca gtgaatattt
     1021 tcggtggcta caaagttcag gggcgcggcg atgaagcggg cgatcaactg ctcagcgatg
     1081 cattagcctt agaagccgct ggggcacagc tgctggtgct ggaatgcgtg ccgattgaac
     1141 tggcaaaacg tattaccgaa gcactggcga tcccggttat tggcattggc gcaggcaacg
     1201 tcactgacgg gcagatcctc gtgatgcacg acgccttcgg cattaccggc ggtcacattc
     1261 ctaaattcgc taaaaatttc ctcgccgaaa cgggcgacat ccgcgcggct gtgcggcagt
     1321 atatggctga agtggagtcc ggcgtttatc cgggcgaaga acacagtttc cattaaggag
     1381 tcacgttgtg ttaattatcg aaaccctgcc gctgctgcgt cagcaaattc gccgcctgcg
     1441 tatggaaggc aagcgcgtgg cgctggtgcc tactatgggt aatctgcacg atggccatat
     1501 gaagctggtc gacgaagcca aagcccgcgc cgatgtggtc gtcgtcagta ttttcgttaa
     1561 cccgatgcag ttcgaccgcc cggaagatct ggcacgttat ccacggacct tgcaggagga
     1621 ctgcgagaag ctaaacaaac gtaaagtgga tttagttttc gccccttcgg taaaagagat
     1681 ctacccgaac ggtactgaaa cccatactta cgttgacgtt cccgggcttt cgaccatgct
     1741 ggaaggcgcg agccgtccgg ggcattttcg cggcgtttcg actatcgtca gcaagctgtt
     1801 taacctggtc cagccggaca tcgcctgctt cggtgaaaag gactttcagc aactggcgct
     1861 aatccgcaaa atggttgccg atatgggctt cgatattgaa attgtcggcg tcccgattat
     1921 gcgcgccaaa gatggccttg cgctcagttc ccgtaatggt tatctgacgg cggaacaacg
     1981 caaaattgcg cccggtctgt acaaagtttt aagttcaatt gccgacaaat tgcaggctgg
     2041 cgagcgggat cttgatgaaa ttattgccat tgcagggcaa gaactgaatg aaaaaggctt
     2101 ccgcgccgat gatattcaga ttcgcgatgc cgacacgttg ctggaagtct ctgaaaccag
     2161 caaacgagct gtaattctgg tagccgcctg gcttggcgat gcccgcctga tcgacaacaa
     2221 aatcgtcgaa ctggcgtaat gcttttactc taaataattc aagttgcagg aaggcggcaa
     2281 gcgagtgaca aattcgtcag gaacgaattt gcccagccgc aggctggcct tcggtgagag
     2341 acatggatgt ctctcattaa tccccaggag cttacataag taagtgactg gggttagcga
     2401 acgcagacgc agcacatgca atttaaagta tgacgagtat aactggcgct acggctgatg
     2461 gcgccaatta ttaatttgcc ccacgtcatg gcgatggcgt ctgtaagcaa ttattcataa
     2521 cgcagacaac ttagttaatc agcctatcaa tctcagcaag agataaattt gtcatctctg
     2581 caacgtcttc ccgagacatc cctttactca gaaaacgctg ggcgaattct tgcctttcct
     2641 cctgtcttcc ctgctgaatc cccttctcaa tccctttctc ttcaaaccac tgcgccagcg
     2701 tcatcataga ctcccctccc gtttccctgt ctctcagcac accataaaac aaatccgctt
     2761 gttcagtatg accgcgttgc agcatatagt tttgcatggc aactaactga cttccgctag
     2821 tgtacccttc gtcaatcagc gtgaccagtt gctccagcaa taacattaag tcgcgctggc
     2881 gaatatgttt ttgcagtagt tcgagaatcg caatccgccg atgttgcatg atttcgtcat
     2941 ccggtgtgat ggtgatatcc accagcggga aaggactgtt atagacgcgt cgtgccagct
     3001 ccggcgagta aaatatatca aaccagcaca ttgagagcgg ataaggtgtg gcctcgccct
     3061 gataaaacag tatcggcacc accagcggca gcttatcgtg atccgcctcc agatgccggt
     3121 gcatggcagc aatagaataa cgcatcatgc gaaagaccat tttcttatcc ggcttgcttt
     3181 ggtgttcaat cacaacatgc agataaccgg gattgccctg catttgcacg gaatagagca
     3241 cgtccgtgct gtgtcctttc aggctctctt caatgaaact ccccgactct aaatgaagcg
     3301 tgttgaggtc acaaagttct cgtaattcca ctggcaaatg tatctccaga aagtcgcgag
     3361 ccgtctccgc gtgcattaaa aattgtttaa acactgcatc atgcggtgtg gtactcggtg
     3421 catccatctt ccctgccttc cgtgttgtct tgcgtgaagg cagtatccag tcaaggcgtg
     3481 ccatcgtcag ccacctcttt agtgtttacc gagcagcgtt cagagaaatt tcaattggtt
     3541 acagccgtta catggcacgc ttcggcacgg tacgcaaact gctgatgtaa atgccgtcag
     3601 atgagtagac actaaacaaa aatcgggcaa tactgcgtga gaatttcctc aaagcaggca
     3661 tcgcctgctt cgttaacgac agggtagaaa ggtagaagtt atgattcgca cgatgctgca
     3721 gggcaaactc caccgcgtga aagtgactca tgcggacctg cactatgaag gttcttgcgc
     3781 cattgaccag gattttcttg acgcagccgg tattctcgaa aacgaagcca ttgatatctg
     3841 gaatgtcacc aacggcaagc gtttctccac ttatgccatc gcggcagaac gcggttcgag
     3901 aattatttct gttaacggtg cggcggccca ctgcgccagt gtcggcgata ttgtcatcat
     3961 cgccagcttc gttaccatgc cagatgaaga agctcgcacc tggcgaccca acgtcgccta
     4021 ttttgaaggc gacaatgaaa tgaaacgtac cgcgaaagcg attccggtac aggttgcttg
     4081 attgttatcc ctgcggctgg ttactcacca gccgcgacat cgtctccagc gaatccgttc
     4141 ttaagatata aagtcgtttt agtaacaacg gattatcccc cggttttact ttgcctttca
     4201 tggttgtcac cgccaggtga aatccggcat cgtttgctgc cttcacggct ttgtcattaa
     4261 atccgccaaa cggatacgaa agataccaga catgcggatt aaattgcgcc agagcgcggc
     4321 gtgaacgtgc aaaatcaaac agaatattgt gctcactacg gctcagtaat atgggtcggc
     4381 gatagccatc cacccgatgc aaaaaatggg tatgtgactg aaaatcaaaa acatcgcgaa
     4441 tttcgttaag ctcagaaacg ctcataaatt gcagcgattt tgggttccac ttctgcgggt
     4501 gacgtttgat gcgcgaggta acaacaaacg ccgtcgcctt catgccatat tgtttcaata
     4561 caggatacgc atagcggctc accgacttga ggccatcatc aaaggtaatc accaccgctc
     4621 gcgcagggag attgatctta ttcttcacgt aaccttccag ctgcgccatg ctcagtgtcg
     4681 cgtatcccct gtcgcgcagc caggccattt ggttattgaa agcgcgtacc gaggtggtcg
     4741 tcgaagtatg gcgaaaacgg gtgttttctt cgtcgcgcag aatatggtga taagttagca
     4801 ccggaaggcc attatcgggt tgggcatcca gcgcgctgat ataggccagt cgatcgccaa
     4861 tacggacctg ataccaggtt tgatttaacc tgtctttcag tttatgcaaa atcgggtagc
     4921 gcaaattgtc cgccaatacc ccaaatggcg cactgcccac actcggcgcg ttatagaccg
     4981 gcgtatcttt ccaggtaact aagttctgat tactcagcgg cttgttgagg tcacccaaac
     5041 cgtcttcaac tttttgtcgc ccctgaaccg gctcaagatg ccctttatcg ataaaccctt
     5101 taccaaagcc aaaattaaat gcgtaataac ttgcggcagt gggctccacg gcaatgattt
     5161 gtccggcccg aatattcccc acggtcacca tcttgtcgcc aatttgcgcc cagaccgcag
     5221 cattttcgat ggtttgcata taacgggcag gtaacgcggc actgacactt gcggtaaaca
     5281 gcatcagcag gagaataaca gcttgtttgt acatgaaaaa ataaccgagg gtgaaacaac
     5341 gcttttattg tagcaaaagc gctataagaa tcgtgggtta atactcatac aaattatgtt
     5401 tgaggacgaa aggcggattt ttttgttgtt ggtgccaaag actactcacc tccggcccac
     5461 ccagttcgac aatacgctcg cgaaactctg aactggtcat ggtttcacgg caagccatca
     5521 tctgttcaat taacggtaac gtgacaccag aaatcacttc gcaacgggag tgtttgtggc
     5581 ttaataatga agccacgcgg tacggtggcg ctccggctat atcggttaag aagatgacac
     5641 cctcaccaga gtccgcttca tgcagagcat cgcacatcat gcggctgagc atattagagc
     5701 ttaacccacg ccagaaattc acggcccggc actgaagaag cgccccatgt tttttctcca
     5761 gcgcatccag tatctcttgc gcccggtcat cgtgacaggt aattacccaa cctaacatcg
     5821 ccttcctcct tagccgaaaa tcagtctacc ctgaccattg ttgataaagg gtgatagccg
     5881 tcaaaactcc tctcccccaa atccagagga gaggaaatag ccttagctac gcaaaccacg
     5941 tccacgttgg atcagcgacc aacagatcaa ataaaacgcc acaataaaga ccaccagtac
     6001 gccaaaggta gtgaccagcg gaacatcatt gataccgagg aagccgtagc ggaaaccact
     6061 gatcatataa acgattgggt tcaggtgcga cagcccttgc cagaacggcg gcaacaaagt
     6121 cagtgagtaa aagaccccac ccaaatacgt gagtggcgtt aacacaaagg ttggcaccag
     6181 gctgatgtca tcgaacgttt tggcaaacac accgttcagc aaacccgcaa gggagaacaa
     6241 caccgccgtg agcaccaggg ttaaggcaac gaatacccac gaatgcacct gaaacggcac
     6301 aaaaaacagt gaaattgccg tcaccagaat gccaacaaac agaccacgcg ccacgccacc
     6361 gccgacatat ccggcaataa tgacgtgagt cggaaccggc gctaccagca gctcttcaat
     6421 attacgctgg aacttggcac caaaaaatga tgacgcaacg ttggcgtagg cattggtgat
     6481 caccgacatc atgatcagcc ccggtacgat gaactgcata tagctgaagc catgcatatc
     6541 gccaatacgc gaaccaatca ggttaccgaa gataataaag taaagggtca tggtgatgac
     6601 tggcggcacc agcgtctgca cccagatacg cataaagcga tggatctctt tcgcccagat
     6661 gctttttagc gccacccagt aaagatgcat catgcgcgat ctccttgttt ttcattaacc
     6721 agtgaaacaa acagctcttc cagacggtta gctttgttac gcatacttaa tacctgaatg
     6781 ccctgctcac ttaactgcgt aaatacgctg ttgatcccct gctcacgcag cacttcaact
     6841 tccagcgtcg cggtatcgac cagtcgatac tgatagccat cgagcttcgg taacggactt
     6901 ttcggtgcga gatcgagaat aaaggtttcc gatttcagct tcgccagcag cgccttcatc
     6961 gaggtatttt ccaccagctc accgtgttga ataatgccga tattgcggca cagcatttct
     7021 gcttcttcca ggtagtgtgt ggtgagaatg atggtggtgc ctttgtcgtt taaatccttc
     7081 aaaaagcccc acattgagcg gcgaagttca atatccacgc ctgcggtcgg ttcgtcgaga
     7141 atcagtagtt taggttcatg cattaacgca cgggcaatca ttaaacggcg cttcatcccg
     7201 ccagataaca tacgcgcacg ttcgttgcgt tttccccata gatcgagttg tttaagatat
     7261 ttttcgctgc ggatgtacgc ctctttgcgc tccacaccgt agtaccctgc ctgattcacc
     7321 acaatttgct gcacggtttc aaacgggttg aagttaaatt cctgcggcac cagtcccaac
     7381 tgacgtttag cgttcacgac atctttctcg agatcgtaac caaatacgct gacccgcccg
     7441 gaggttttat ttaccagaga gctgataata ccgatagtgg tcgatttccc ggccccgttc
     7501 ggcccgagaa gcgcataaaa atcacccgct tcgacctgca aatctattcc acgaagcgcc
     7561 tgaacgccgc ctggataggt ttttttaagc tgttgaagtt ccagtgcaat ggtcataaat
     7621 ttttacttac cttacgttct tacactttat ctgtggttta aatcgtcccg gagttgccct
     7681 atattagcca aacgtaatta tttggttaca ggtcgttaac ctccatgaaa gacatagata
     7741 cactcatcag caacaatgca ctatggtcaa aaatgctggt ggaagaggat cccgggtttt
     7801 ttgagaaact ggcacaagcg caaaaaccgc gctttctatg gattggatgt tccgacagtc
     7861 gcgttcctgc agaacgttta accggtcttg agccgggcga actctttgtt caccgtaatg
     7921 ttgctaacct ggtcattcac accgacctga actgcctttc cgtggttcag tatgcagtgg
     7981 atgtactcga agttgaacac attattatct gtggccacta cggttgcggc ggcgtacaag
     8041 ccgccgttga aaacccggaa ctggggctta tcaacaactg gctgctgcat atccgcgata
     8101 tctggttcaa acatagctca ttgctcggcg aaatgccgca agagcgccgt ctggatacct
     8161 tgtgtgaact gaacgtcatg gaacaggttt ataacctagg ccactccacc attatgcaat
     8221 cagcgtggaa acgcgggcag aaagttacca ttcacggctg ggcctacggc attcacgacg
     8281 gcttgctgcg tgatctggat gtcacagcga ccaaccgcga aacccttgag caacgttacc
     8341 gtcacgggat ttccaacctc aagctgaaac acgccaacca caaataaaaa tgccatgccg
     8401 gatgcaacac atccggcaac ttcacactta ctcgtcccgc agaatcactt tgccgatata
     8461 cggcagatga cggtaacgct gtgcgtaatc aatgccgtaa cccaccacaa actcatccgg
     8521 gatcgagaaa ccgataaatt ctaccgggac gttcacttca cgacgggacg gtttatccag
     8581 cagcgtacaa atcgccagcg acttcggttc gcgcaggctt aagatctcac gcactttcga
     8641 cagtgtattc cccgagtcga tgatatcttc aacaatcagc acgtccttgc cacggatatc
     8701 ttcatccaga tctttgagga ttttcacatc acgggtggtg gacatgccgc taccgtagct
     8761 ggaggcggtc ataaagtcga cttcatgaga tacctgaact tcacggcaca ggtccgccat
     8821 aaacataaat gagccacgca gcagacccac cagcaccata tcgctgccgc tgtctttgta
     8881 acgctcagta atctgacgac ccagttcggc gatacgcgct ttaatctccg cttcggggat
     8941 cattacttct acagtatgtt tcatatctct aaccatatga tttaaaaaca aatcacttcg
     9001 cacgggcagg ttttggcacc gcaacaaagc gcaagacacg cagtatacca gtaaaactat
     9061 tcattcgcgg aatctgttaa taaagctcga attgtgatga cggtcacaca tgttaaaacc
     9121 tataattaat tgctactaaa atattaatga attgaaatgg tgtctcttta tggcaattaa
     9181 caatacaggc tcgcgacgat tactcgtcac gctaacagcc ctttttgcag cgctttgcgg
     9241 gctgtatcta ctcattggcg gaggctggct ggtcgcgatt ggcggctcct ggtactaccc
     9301 tatcgctggc cttgtgatgc tcggcgtcgc ctggatgctg tggtgcagta aacgcgccgc
     9361 gctttggcta tacgcagccc tgctgctcgg caccatgatt tggggcgtct gggaagttgg
     9421 tttcgacttc tgggcgctga ctccgcgcag cgatattctg gtcttcttcg gcatctggct
     9481 gatcctgccg tttgtctggc gtcgcctggt cattcctgcc agcggcgcag ttgccgcact
     9541 ggtggtggca ctgctgatta gcggtagtat cctgacctgg gccggattta acgatccgca
     9601 ggaaatcaac ggcaccttaa gcgccgatgc cacacctgcg gaagctatct cacccgttgc
     9661 cgatcaggac tggcctgcct atggtcgcaa tcaggaaggt caacgctttt cacctctgaa
     9721 acaaattaac gccgataacg tccataatct gaaagaagcc tgggtgttcc gtactggcga
     9781 tgtgaagcag ccgaacgatc cgggcgaaat caccaatgaa gtgacgccga ttaaagtggg
     9841 cgacaccctt tacctgtgta ccgctcacca gcgcctgttt gcgctcgatg ccgccagcgg
     9901 caaagagaaa tggcattacg atcctgagct gaaaactaac gagtctttcc agcacgtaac
     9961 ctgccgtggc gtctcttatc atgaagccaa agcagaaacg gcttcgccgg aagtgatggc
    10021 ggattgcccg cgtcgtatca ttcttccggt caatgatggt cgactgattg cgattaacgc
    10081 tgaaaacggc aaactgtgcg aaaccttcgc caataaaggc gtgctcaatc tgcaaagcaa
    10141 tatgccagac accaaaccgg gtctgtatga accgacttcg ccaccgatta tcaccgataa
    10201 aaccatcgtg atggccggtt cagttaccga taacttctca acccgcgaaa cgtctggcgt
    10261 gatccgtggt tttgatgtca acaccgggga gctgctgtgg gcttttgatc ccggcgcgaa
    10321 agatccgaac gcaatcccgt ctgacgaaca cacctttacc tttaactcgc caaactcctg
    10381 ggcaccagcg gcctatgacg cgaagctgga tctggtctat ctgccgatgg gcgtgaccac
    10441 gccggatatc tggggcggta accgcacacc ggaacaggaa cgttatgcca gctcgattct
    10501 ggcgctgaat gccactaccg ggaaactggc gtggagctac cagaccgttc accacgacct
    10561 gtgggacatg gatcttccgg cacagccgac gctggcggac atcaccgtta atggtcagaa
    10621 agtgccagtt atttacgctc cggcgaaaac cggcaacatt tttgtgctcg atcgtcgcaa
    10681 tggcgaactg gtggttccgg ccccggaaaa accggtgccg caaggtgctg cgaaaggcga
    10741 ttacgttact ccgacccagc cgttctctga actgagcttc cgtccgaaga aagatttgag
    10801 cggtgcggat atgtggggag ccaccatgtt tgaccaactg gtgtgccgcg tgatgttcca
    10861 ccagatgcgc tatgaaggca ttttcacgcc gccatctgaa cagggcacgc tggtcttccc
    10921 gggtaacctg ggaatgtttg aatggggcgg gatttccgtt gatccaaatc gtgaagtggc
    10981 aattgccaac ccaatggcac tgccgtttgt ttcgaaactg atcccacgcg gtccgggtaa
    11041 cccgatggag cagccgaaag atgccaaagg cacgggtacg gaatccggca ttcagccaca
    11101 gtacggtgta ccgtatggtg tcacgctcaa cccgttcctc tcaccgtttg gtctgccgtg
    11161 taaacagcca gcatggggtt atatctcggc gctggatctg aaaatcaatg aagtggtgtg
    11221 gaagaaacgt attggtacgc cgcaagacag tatgccgttc ccgatgcctg taccggtgcc
    11281 gttcaatatg ggtatgccga tgctgggcgg gccaatctcc acggcgggta acgtgctgtt
    11341 tatcgctgct acggcagata actacctgcg cgcttacaac atgagcaacg gtgaaaaact
    11401 gtggcagggc cgtttaccag cgggtggtca ggcgacgcca atgacctatg aagtgaatgg
    11461 caagcagtat gtggtgatct ccgcaggcgg tcacggttca tttggtacga agatgggcga
    11521 ctatattgtg gcttatgccc tgccggatga tgtgaaataa ccataatgcc cggagagatc
    11581 cgggcatatt tccgaatacg gtctttttat accgtaaacc ctaacatcat ccccgtatct
    11641 tcatgctcca gcagatggca gtgcgccata taagcatgtt ctttcggtgc gttgtgatta
    11701 aacttcacca gcacttcact gacattgcct tctaccttaa cggtatcttt ccagcccgcg
    11761 cgatgagccg ctggcggttt gccattttct gacaagatac ggaactgcat gccgtgaata
    11821 tggaacggat gcagcatcat gtcgcccacg ccagagataa cccaacgttc gtactgccct
    11881 ttcgccgccg caaacatcgg cttattcata tcaaacgcct gaccgttgat tttattggca
    11941 tggtggaaat cgaacttccc gccgtggttc atatgattca tattgccgtg ccccatatgg
    12001 cccatcatct ggctgtgatc cattccggcc atcgcctgat cgccatattt ctccattagc
    12061 atctgcatcc ccatcatatc gagcatcggg tccatagaaa gttgcagctt gcgtaccgtc
    12121 agcccttcca gcgaaggtaa cgcaggcagg ctacttaatg tgtcaggcaa agcaccggag
    12181 gcactaatag caatcggctg aatccgcatt accggatgcg gcttgtcaaa cggcgcaatc
    12241 gccatcccca tctggctgac cggtagcgtc accagatcaa agggtttgtt gtcgttaacc
    12301 tccaccagca cttcaaaacg ctcgcccatc agcaccggca gctcgctcac cttcaccggt
    12361 tcaggtagca gaccaccgtc gctggcaatc acatacagcg ggcgattgtc gctggtggcg
    12421 aaattgagcg agcgggcgtt acagccattg agcaaacgca ggcgcagcca gccacgcggg
    12481 gcagcgtgtt gtgggtagat tgcaccgttg gtcagcagcg tatcgccaaa ccagcccacg
    12541 gcggcggtca tcacatccag ttgataatca atctgcccat cagcgttaaa ttttttatcc
    12601 tgaacaatca ccggaacatc atcgataccc cactgttttg gcagcattaa tttcaggatc
    12661 tcgtcatctt caattaccac cagcccagcc aaccccatcg ccacctgtcg cccggtcttg
    12721 ccatgttgat gtggatggaa ccagcaggta gcggcaggtt gatcaacgtt caacgtcacc
    12781 gtacgtttgc cgcctggcgg aataattccc tgcgggccgc cgtcgacgtc acccggtatt
    12841 tccagcccgt gccagtgcaa cgtcgtctct tccgccagtt ggttatagat atcaaccgtt
    12901 accgctttgc cgcgctgtaa tttcaccgcc ggccccagca gattgccgtt atagccccag
    12961 gtagttgcag ttttctcgcc aaaggtagac tgacctgcgc caatagttaa ctgaatgcga
    13021 ttacgggcat cggtcgtgag caaatcaggg atcggtaacg ttgggcgttc cgccgcaaag
    13081 actgcgcggc tccacagcgg caaggctgaa gccacaccca gcgcgacgga atattttaag
    13141 aaatcacgac gttgcatagt tatttcctta ttcttaagca ggcgaaacaa aatcaaacgt
    13201 tgagcatagt ccttcccctt acgggaaggt caagccgcag acacaattta atcgacaata
    13261 atggtcgtcg cctcgctcgc agtgtgctaa cgtttatctt ctttaagccc ctggtagaag
    13321 caatgaagac gtttttcaga acagtgttat tcggcagcct gatggccgtc tgcgcaaaca
    13381 gttacgcgct cagcgagtct gaagccgaag atatggccga tttaacggca gtttttgtct
    13441 ttctgaagaa cgattgtggt taccagaact tacctaacgg gcaaattcgt cgcgcactgg
    13501 tctttttcgc ccagcaaaac cagtgggatc tcagtaatta cgacaccttc gacatgaaag
    13561 ccctcggtga agacagctac cgcgatctca gcggcattgg cattcccgtc gctaaaaagt
    13621 gcaaagccct ggcccgcgat tccttaagcc tgcttgccta cgtcaaataa tccctgatac
    13681 ctttttgtag aaatattgac cgtgcatccg cggtcaatgt tagctattat gttgcgccct
    13741 ttttttacgg gtgttaacaa aggaggtatc aacccatggc cgaaaaaaaa cagtggcatg
    13801 aaacgctaca cgaccagttt gggcagtact ttgcggtaga taacgttctg tatcatgaaa
    13861 agaccgatca ccaagatctg atcatttttg agaacgctgc atttggtcgc gtaatggcgc
    13921 tggatggcgt agtacaaacc accgagcgcg acgagtttat ctatcatgag atgatgaccc
    13981 atgttccgct actggcccac ggtcacgcga aacatgtgct gattatcggc ggcggcgacg
    14041 gtgccatgct gcgtgaagta acccgacata aaaacgttga gtcaatcacg atggtggaaa
    14101 tcgatgcggg tatcgtgtcg ttctgccgtc agtatctgcc caaccataac gccggtagct
    14161 acgacgatcc gcgctttaag ctggtgatcg acgatggcgt caatttcgtt aatcaaacca
    14221 gccagacctt tgatgtcatt atctccgact gcaccgatcc tatcggtccc ggcgaaagtc
    14281 tgttcacctc agcattttat gaaggctgca aacgttgcct gaatcctggc ggtatcttcg
    14341 tcgcacaaaa cggcgtctgc tttttacagc aggaagaagc catcgacagc tatcgcaaac
    14401 tcagccatta cttcagcgac gttggctttt atcaggcggc gatcccgacc tattacggcg
    14461 gtatcatgac ttttgcatgg gcgacagata acgacgcctt acgccatctc tcaaccgaaa
    14521 ttattcaggc gcgttttctc gcttctggcc tgaaatgccg ttattacaat ccggcaatcc
    14581 atacggcagc ttttgcctta cctcagtatc tgcaagacgc actggcttca cagccgtcct
    14641 aaggagaaga taagaaattg aaaaaactga aactgcatgg ctttaataat ctgaccaaaa
    14701 gtctgagttt ttgtatttac gatatctgct acgccaaaac tgccgaagag cgcgacggtt
    14761 atattgctta tatcgatgaa ctctataatg ccaaccgtct gaccgaaatc ctgtcagaaa
    14821 cctgttccat tatcggggcc aatattctta acatcgcccg ccaggattac gaaccacagg
    14881 gtgccagcgt cactattctg gtgagtgaag aaccggttga cccgaaactc atcgacaaaa
    14941 cagaacaccc cggcccactg ccagaaacgg tcgttgccca tctcgataaa agtcatattt
    15001 gcgtacatac ctacccggaa agtcatcctg aaggcggttt atgtaccttc cgcgccgata
    15061 ttgaagtctc tacctgcggc gtgatttctc cgctgaaggc gctgaattac ctgatccacc
    15121 agcttgagtc cgatatcgta accattgatt atcgcgtgcg cggttttacc cgcgacatta
    15181 acggtatgaa gcactttatc gaccatgaga ttaattcgat tcagaacttt atgtctgacg
    15241 atatgaaggc gctgtatgac atggtggatg tgaacgtcta tcaggaaaat atcttccata
    15301 ccaagatgtt gcttaaagag ttcgacctta agcactacat gttccacacc aaaccggaag
    15361 atttaaccga cagcgagcgc caggaaatta ccgctgcgct gtggaaagaa atgcgcgaga
    15421 tttattacgg gcgcaatatg ccagctgttt aacggctctg gcggagctcc caggctccgc
    15481 cagatttatt tacttctgct gcacgaaatt gcggtaagcc gccacgacct gcagaaaatc
    15541 ctcaacgccg catagcgaca ggctttcttc gtcgtagtag ttcatccctt cttccatttc
    15601 atcgccagcg aattccagtt gattggcgcg aaccatcacc tcttcaccgt ccatccacag
    15661 ggtgtattca tgccctgccc gttgccagga ccgttcgcta cctttcagtg cgtgcgctgc
    15721 ttgttccact tcatcaagca aagccaggtt ttctttcacc tcttcattaa accagtgccc
    15781 gaccacttca tgccccatgg acatacgcac ctttaccact ccggtaatat cgcgcagaaa
    15841 ttcgtaatcc atagtgtgtt cctctgctcc cggcaatgcg ccgaaatcgc attgcgctat
    15901 tgatgtaatt attgcagcct taccggagaa aaacagcgga acggcgatgg tttagaaata
    15961 aaaaatgccc gggaatctca ttcaccgggc attgtgtcgt ttatgcgcag cgcgtgcgct
    16021 tacttattac accgcagtct ggaaaatcac cccatcggct ttttcggtgt actgagaaag
    16081 ctggttgaag ttcagataac gataagtatc aacggcagtt ttatctactt gcgcaacgta
    16141 ggtctggtac tcttccggcg tcggcagttt gccaatcagc gccgcaacag ccgccagttc
    16201 tgcagaagcc aggaagacat tcgcgccagt ccccagacgg ttcgggaagt tacgggtaga
    16261 ggtggaaacc accgtcgcac cgtctgctac acgcgcctgg ttacccatac acagagaaca
    16321 gcccgggatc tcgatacgag ccccgctctt accgaagacg ctgtagtagc cttcttcagt
    16381 caactgtgcg gcgtccatgc gggttggcgg tgccacccac aggcgggtcg gcaactggcc
    16441 tttgtgcgca tccagcagtt tacccgcagc acggaagtga ccgatgttag tcatgcaaga
    16501 accgataaac acttcgtcga tcttctcgcc ctgtaccgca gacagcggac gtgcgtcgtc
    16561 cgggtcgttc ggtgcacaga ggattggctc tttaatatcc gccagatcga tgtcgatcac
    16621 tgccgcgtat tcagcatctg catcggcttc cagcagctca ggattcgcca gccatttttc
    16681 catgccctga atacgacgtt cgagggtacg acgatcgcca taaccttccg cgatcatcca
    16741 cttcagcagg acgatgttag agttcaggta ttcgatgatc ggttctttgt tcagcttgat
    16801 agtacaacca gcggcagaac gctcagcgga cgcatcggtc agttcaaatg cctgctcaac
    16861 tttcagatcc ggcagacctt caatttccag aatgcgacca gagaagatgt ttttcttgcc
    16921 tttcttctca acggtcagca gaccttgttt gatcgcatac agcgggatcg cgtgtaccag
    16981 atcgcgcagg gtgatgcccg gctgcatttt gcctttgaag cgcaccagaa cggattccgg
    17041 catatccagc ggcattacgc ctgttgcggc tgcaaatgcc accagaccag aacccgccgg
    17101 gaaagagata ccgatcggga aacgggtatg ggagtcacca ccggtaccga cggtatccgg
    17161 cagcagcata cggttcagcc aggagtggat tacgccgtca cccggacgca gcgacacacc
    17221 accacggttc ataatgaagt ccggcagcgt gtggtgcgtg ttcacgtcaa ctggcttcgg
    17281 atacgccgcg gtatggcaga aagactgcat caccaggtca gccgagaagc ccaggcacgc
    17341 caggtctttc agttcatcac gggtcatcgg gccggtggtg tcctgtgaac ctacagaagt
    17401 cattttcggt tcgcagtacg cgcccggacg aatacctttc acaccgcagg cacgacctac
    17461 cattttctga gccagcgaga agccgcgatc gctctcagcg acatctttcg cctgacggaa
    17521 cacatcactg tgcggcagac caagtgcttc acgcgctttg gtggtcaggc cacgcccgat
    17581 aatcagcgga atacggccac cagcacgcac ttcatcaatc agcacgtcgg ttttcagttc
    17641 gaaggtcgcc agcagttcgc cggtttcgtg gttacgcact tcacctttgt acgggtaaac
    17701 gtcaatcacg tcgcccatgt tcaggttaga gacgtcgact tcgattggca gtgcacccgc
    17761 gtcttccatc gtgttaaaga agatgggtgc aattttaccg ccgaggcaca aaccaccgcc
    17821 gcgtttgttc ggcacatgtg gaatatcatc gcccataaac cacagaacgg agttcgtggc
    17881 ggatttacgc gatgaacccg tacccacaac gtcaccgacg tacgccagcg ggaaaccttt
    17941 ctgttgcaga gcttcgattt gcttgatcgg gccaacaaca cctggctggt ctggctcaat
    18001 accttcacgg gcgtttttca gcatcgccag cgcgtgcagt gggatatccg ggcgcgacca
    18061 cgcatccggt gccggagaaa ggtcatcagt gttggtttcg ccagtgactt tgaagacggt
    18121 aacggtcagt ttttcagcca gcgccgggcg attcaggaac cattcggcat ccgcccagga
    18181 ctgcattacc tgcttcgcat attcgttgcc tgctttggct ttctcttcta cgtcatagaa
    18241 gttatcgaac atcagcagtg tgtgagaaag tgctttggca gcgatcggtg ccagtttggc
    18301 atcatccagc gcgtcgatca gcggatgaat gttgtaacca ccctgcatgg tacccagcag
    18361 ttcgatggct ttttccggag tcagcagagg ggatttggct tcgcctttag cgatagcagc
    18421 caggaagcct gctttgacat aggcggcttc atcgacgcca gggggaacac ggttggttaa
    18481 tagatctaac aggaattctt cttcgcccgc gggcgggttt ttcagcagct ctacaagtgc
    18541 ggccatttgg tttgcatcca ggggtttggg cgcaatcccc tcagcggcac gctcagctac
    18601 gtgcttacgg tattcttcta gcacgacggt tctcctcgct ctcattgtca tagtgcggca
    18661 ggcgattctc ttcacgctcc tgtgagacag cagtttgtag ggtaaatgcc cagtaccgca
    18721 acgggcagaa tagcaggatt ttgacgacgt gttaatctgt ttacaaaaaa gcaacataaa
    18781 aaagtggctg aatcgttaag gatggtctag agattgtttc cctccgtaaa aacttcgtta
    18841 attcgcatgg tgataatcac ctttctcaac gaatcccaag attagtcaaa atttaaacta
    18901 ccgcctcttt atactcggat taacagcacc tgcgggtggc agttcgccga ccattgcgat
    18961 ttccttgaga tccgaattat gaaaatgact ttgccgttta aaccccatgt gctggcacta
    19021 atttgcagtg ccgggctttg tgccgcctct gccgggctat atataaaaag ccgcacagtg
    19081 gaagcgcctg tggaaacgca atcgacacaa cagactgcgc ctgacatcac cgcagttacg
    19141 cttcctgcaa cggtttccgc accgcccgta acgcccgccg tcgtcaaatc cgcattcagc
    19201 actgcacaaa tcgatcaatg ggttgcgcct gtcgcgctgt accccgattc tctgctttca
    19261 caagtgttaa tggcatcaac ctatccggca aacgttgctc aagcagtgca atggtcgcac
    19321 gataatccac ttaaacaagg cgatgctgct attcaggcgg tatctgacca gccgtgggac
    19381 gccagcgtta aatcactggt ggcctttcca caattgatgg cattgatggg cgaaaacccg
    19441 caatgggtgc aaaacctggg cgatgctttt ctggcgcagc cgcaggacgt gatggactcg
    19501 gtacagcgat tgcggcaact ggcgcaacaa accggctcgc tgaagtcatc aaccgaacag
    19561 aaagttatta ccacaacgaa gaaagctgta ccggtaaaac agacagtcac ggcacccgtc
    19621 ataccatcca ataccgtttt aactgccaac cccgtcatta cagagccggc aacaaccgtc
    19681 atttccattg agcccgccaa tcctgatgtg gtctatattc ccaactacaa cccaaccgtg
    19741 gtttacggga actgggccaa tactgcgtat ccgccggttt atctgccacc accagccgga
    19801 gaaccgtttg ttgacagctt tgtgcgcgga ttcggctata gcatgggcgt tgctaccacg
    19861 tacgcactat ttagcagcat cgactgggat gacgacgatc atgaccatca tcatcatgac
    19921 gatgatgatt atcatcacca cgatggcggt catcgtgacg gtaatggctg gcaacacaac
    19981 ggcgacaaca tcaatatcga cgtcaacaat ttcaaccgta tcaccggtga gcatcttact
    20041 gataagaata tggcatggcg gcacaatcca aactaccgta atggtgtgcc ctatcatgat
    20101 caggatatgg caaagcggtt tcatcaaacc gatgtcaacg gcggaatgag tgccacgcag
    20161 ctacctgctc caacacgcga cagccagcgt caggcggcag caagtcagtt tcagcaacga
    20221 acacacgccg cccccgtcat tacacgagat acccaacgtc aggcagcggc acagcggttt
    20281 aatgaagctg aacactatgg gagctatgac gacttccgcg acttcagccg tcgccaacca
    20341 ctgacccagc aacaaaagga cgccgctcgt cagcgttatc aatcggcctc tcctgagcag
    20401 cgccaggcgg ttcgcgagaa aatgcagact aacccacaga accagcagcg aagagaggca
    20461 gcgcgtgagc gtattcagtc tgcaactccc gaacagcgcc aggtgtttaa ggaaaaagta
    20521 cagcagcgcc cactgaacca acagcaacgt gataacgccc gccagcgtat acaatcggca
    20581 tcacctgaac aacgtcaggt ttttcgggaa aaagttcagg agagccgccc acaacgtcta
    20641 aacgacagta accatactgt caggctgaat aacgagcaac ggtcagcagt acgcgaacgt
    20701 ctctctgagc gcggagcaag gcgacaggaa aggtaaattg cagacgtaaa aaaagcggcg
    20761 tggttagccg cttttttaat tgccggatgt tccggcaaac gaaaaattac ttcttcttcg
    20821 ctttcgggtt cggcaggtcg gtaatgctac cttcgaacac ttctgccgcc aggcccacag
    20881 actcgtgcag agtcgggtgc gcgtggatgg tcagtgcgat gtcttcagca tcacaaccca
    20941 tttcgattgc cagaccgatt tcacccagca gctcaccgcc gttagtaccg acaatcgcac
    21001 caccgatcac acggtgagat tctttgtcga aaatcagctt ggtcataccg tctgcgcagt
    21061 cggaagcgat agcacgacca gaagcagccc acgggaaggt ggcggtttca tagctgatgc
    21121 ctttctcttt cgcttctttc tcagtcagac ctacccatgc aacttctggt tcggtatagg
    21181 cgatagacgg gataactttc ggatcgaagt agtgtttttt accggcgata acttcagcgg
    21241 caacgtgacc ttcgtgaaca cctttgtgtg ccagcatcgg ctgaccaacg atatcgccga
    21301 tagcaaagat gtgcggtacg ttggtacgca gctgtttgtc aacgcggatg aaaccacggt
    21361 cgtccacttc cacgccagct ttgcctgcgt cgaggttttt accgttcggc acacgaccaa
    21421 tcgctaccag cacggcgtcg taacgctgcg gttcagcggg tgcttttttg ccttccatcg
    21481 tcacataaat accgtcttct ttcgcttcaa cggcggtaac tttggtttcc agcatcaggt
    21541 tgaatttctt gctgatacgc ttggtgaaga ctttaacgat gtctttgtca gctgccggga
    21601 taacctggtc gaacatttca accacgtcaa tctgtgaacc cagcgcgtgg tatacggtgc
    21661 ccatttccag accgatgata ccgccaccca ttaccagcag gcgttctggt acttctttca
    21721 gttccagcgc gtcagtggag tcccagatac gcggatcttc atgcggaata aacggcagtt
    21781 ggatcgggcg agaacccgct gcaatgatcg cattgtcgaa gttgatcacg gttttgccgt
    21841 tctcaccttc aacttccagg gtgttagccc cggtgaattt acccagaccg ttgaccactt
    21901 tgactttgcg gcctttcgcc ataccagcca gaccaccggt cagctgattg atcactttct
    21961 ctttccaagt acgaatcttg tcaatatcgg ttttcggttc gccgaagacg ataccgtgtt
    22021 cagccagcgc tttggcttct tcgataactt ttgctacgtg cagcagtgct ttagaaggga
    22081 tacagccgac gttcaggcaa acaccgccaa gggtgttgta acgttctacg attacggttt
    22141 ccagacctaa atcagcgcaa cggaaggcag cggagtaacc tgcggggcct gccccaagta
    22201 ccacgacctg agttttgatt tcagtactca tcatgacctc tatatattta tctccggcgg
    22261 tcatacccgt cgtctttcag gccgcagcgg cgttagcttc acgccctcac cccagtcact
    22321 tacttatgta agctcctggg gattctggcg cttgctacct tgctgcaccc agaaatccat
    22381 agggtatgtg aagtaatacc ctaaccacca ccgggtcgtt ctatccgtcg gtattttaca
    22441 aaattgttaa caatttttaa acaacaaacg gcaaccgatt tgtctattcg ctaataacct
    22501 caatacattc atgagattac cagaaaaaag ccggccgttg ggccggctct tttacttaca
    22561 tcaccagacg gcgaatgtca gacagcgtgt tgttaatgat ggtaatgaaa cgggcaccat
    22621 cagcaccgtc gatcacgcgg tggtcgaagg agagagaaat cggcagcatc agacgcggca
    22681 cgaactcttt accattccac accggctcca tcgcggactt ggaaacgccg aggatagcca
    22741 cttccggcgc gttcacaatc ggcgcgaagt gggtagtacc caggccgccg atgctggaga
    22801 tggtgaagca accgccctgc atttcgcccg cagtcagctt accgtcacgc gctttcttag
    22861 aaatagtcat cagctcgcga gacagctcga tgatgccttt cttgttgacg tctttgaata
    22921 ccggaacaac cagaccgttc ggggtatcca ccgccacacc gatgttgatg tatttcttca
    22981 gggtcagacg ctgaccgtct tccgacagcg aactattgaa gcgaggcatc tgctcaagag
    23041 ctgcagcaac ggctttcatg atgaagacaa ccggggtgag cttcacatcc agcttacgtt
    23101 tcgccgcttc ttcgttctgc tgtttacgga acgcttccag ctcggtgata tcggttttgt
    23161 cgaagtgggt aacatgcggg atcatcaccc agttacggct caggttagca ccagaaattt
    23221 tctggatacg gcccagttcc acttcttcga tttcgccaaa cttgctgaag tccaccttcg
    23281 gccacggcag catgccaggg ataccaccgc cagtcgccgc cggagctgct tctgcacgtt
    23341 tgatagcttc tttcacgtaa gcctgaacgt cttcgcgcag gatacgacct ttacgaccag
    23401 tgcccttcac tttcgccagg ttaacgccaa actcgcgtgc cagacggcgg atcagcggag
    23461 tcgcgtgaac gtaagcgtcg ttttcagcaa attcagattt gccttcggct ttcgcagccg
    23521 gagctgctgc cggggcttca gctttcgctg ccggagccgg cgctgccgct tcctgtttcg
    23581 caggagctgc cgcaggcgct gcgccttcaa cttcgaagat cataatcagc gagccagttt
    23641 tcactttatc gccaacgttg actttcagtt ccttcacgac gcctgcaaac ggagccggga
    23701 cctccataga agctttatcg ccttctacgg tgataagcga ttgttcagcg gcaactttgt
    23761 cgcccacttt caccatcact tcggtcactt caacttcgtc accgccgata tccggaacgt
    23821 taacttcttt cacgccagcc gctgatgcag gggccgctgc cggagctgct tcctgtttag
    23881 ccgccggagc tgccgcgcct gcttcacccg cgacttcgaa gaccataatc agcgagccgg
    23941 tagacacttt gtcacccacg ttcactttga tctctttcac ggtgccagca aacggagccg
    24001 gaacttccat agaagcttta tcgccttcta cggtgatcag cgactgttca gcttcaactt
    24061 tatcgccaac tttcaccagg atttcggtca cttcaacttc gtcgctgccg atatccggaa
    24121 cgttaacgtc ttttgccgcc gcagccgcgg gtgctgctgc cggagctgct tctttcttct
    24181 cttctgcctg agcaggtgca gcgtctgctg caccgtcggc ggaatcgaaa atcataatca
    24241 gtgcgccggt ctgggtttta tcgccaacag agactttgat ctctttaacg atacccgcct
    24301 gcggagacgg aacttccata gaggctttgt cgccttctac ggtgatcagc gactgttcgg
    24361 cttcaacttt gtcgcccact ttgaccagga tctcggtgat ttcaacttca tcagccccga
    24421 tgtccggtac tttgatttcg atagccatta ttcttttacc tcttacgcca gacgcgggtt
    24481 aactttatct gcatcgatgt tgaatttggc gattgcgtca gcaaccactt tcttatcgat
    24541 ttcgccacgt ttagccagtt cgcccagcgc cgcaaccacg acataagaag catcaacttc
    24601 gaagtggtga cgcaggttct cacggctgtc ggaacgaccg aagccatcag tacccagtac
    24661 gcggtagtcg tcagccggta cgtaagtacg gacctgctca gcgaacagtt tcatatagtc
    24721 ggtagatgcc actgccggag cgtcgttcat cacctgagcg atatacggaa cgcgcggagt
    24781 ttccagcgga tgaagcatgt tccagcgttc acaatcctga ccatcacgcg ccagctcggt
    24841 gaaggaggtc acgctataaa cgtcagaacc tacgccgtaa tctttcgcca ggatctctgc
    24901 cgcttcacgg acgtgacgca ggatagaacc ggagcccagc agctgaactt tacctttgct
    24961 accttcaatg gtttcgagtt tgtagatacc tttacggata ccttcctcag caccttccgg
    25021 cattgccggc atgtggtagt tttcgttcag cgtagtgatg tagtagtaaa cgttctcttg
    25081 tttttcaccg tacatacgct ccagaccgtc atgcatgatg acagcaactt cgtaagcgta
    25141 agccgggtcg taagagatac agttcgggat agtcagcgac tgaatgtggc tgtgaccatc
    25201 ttcgtgctgc agaccttcac cgttcagggt ggtacgaccg gaagtaccgc cgatcaggaa
    25261 gccacgcgct tgctggtcgc cagccgccca gcacagatcg ccgatacgct ggaagccgaa
    25321 catcgagtaa tagatgtaga acggaatcat cggcagattg ttggtgctgt aagaggtcgc
    25381 cgctgccagc caggaacacc ctgcgcccag ctcgttgatc ccttcctgca gaatctgacc
    25441 tttctcgtct tctttatagt aagcaacctg ctcgcggtcc tgcgggatgt actgctgacc
    25501 gttcgggctg taaataccaa tctgacggaa cagaccttcc ataccgaaag tacgcgcttc
    25561 gtcggcgatg atcggtacca gacgatcttt gatcgacttg ttcttcagca ttacgttcag
    25621 ggcacgaacg aaagcgatag tggtagagat ctctttgctc tgctcttcca gcagcgcgcc
    25681 gaagtcttgc aggctcggca gctcaagctt ctcggtgaag ttcggctgac ggcttggcag
    25741 ataaccgtgc agtttctgac gctgtgcgtg cagataggta tgctcttcag aaccttccgg
    25801 gaaggtgatg tacggcagtt tttcgatatc tgcatcagac accggcacat tgaaacggtc
    25861 gcggatgtga cgcacgccgt ccatgttcat tttcttaacc tggtgcgcga tgtttttacc
    25921 ttcagccgcg tcgcccatgc cgtaaccttt aatggtatga gcaaggatta ctgtcgcttt
    25981 gcctttggtt tcctgcgctt tcttgaatgc agcgtagatt ttcttcggat cgtgaccacc
    26041 acggttcagt gcccagatct gctcgtcagt ccagtctgca accagtgctg cggtttcagg
    26101 atatttaccg aagaagtgtt cacgaacgta cgcaccatct ttcgatttga aggtctggta
    26161 gtcgccgtca acggtttcgt tcatcagctg gatcagttta ccgctggtat ctttacgcag
    26221 cagttcatcc caacggctac cccacatcac tttgataacg ttccagccag caccttcgaa
    26281 gatgccttcc agttcgttga tgatcttgcc gttaccggtg accgggccgt caagacgctg
    26341 caggttacag ttgataacga agaccaggtt atccagtttt tcacgggtag cgatggtgat
    26401 cgcacctttg gattccggtt cgtccatttc accgtcgccg aggaacgcgt aaacggtctg
    26461 tttagaggta tctttcaggc cacggtgttc cagatatttc aggaatttag cctggtaaat
    26521 agcaccaatc ggccccagac ccatagatac ggtcgggaac tgccagaatt ccggcatcag
    26581 tttcgggtgc ggataggaag agaggccatt gccgtgaact tcctgacgga agttatccag
    26641 ctgctcttga gtcagacgac cttccaggaa agcacgagcg tacacgcccg gggagatgtg
    26701 gccctggaag taaaccaggt cgccgccatc ctgctcgttg cgtgcacgga agaagtggtt
    26761 aaagcacaca tcataaatgg ttgcggaaga ctggaaggac gccatatggc cgcccagttc
    26821 gaggtctttt ttcgacgcac gcagcaccgt catgatggcg ttccagcgga tagctgaacg
    26881 aatacggcgt tccagttcca gattacccgg atactccggt tgttcttcaa cggggatggt
    26941 gttgatgtag ttgctgatac ctgtgcctgc ggctacgttt acaccgcctt tgcgggcttc
    27001 agcaagcagt tggtcgatca gatactgagc acgctcaaca ccttcttcac ggatgaccga
    27061 ttcgatcgcc tggagccagt cgcgagtttc gatcggatcc acgtcatttg ggaaacgttc
    27121 tgacatgggt tattccttat ctatctaata acgttgagtt ttctggaacc tgtcccattg
    27181 aactctcgcc ggaaagctca ataagacagg ttctacgttt agttgccgcg cttttatata
    27241 cgcttgattt acaacatctt ctggataatt tttaccagaa aaatcactaa ttctttcgtt
    27301 gctccagacg acgcagagaa cgctcacggc ggctctcttc acgacttctg tcgagcaaaa
    27361 tttcttcgat aaaggccaga tggcgatgcg atgcttcgcg cgcttcttcc ggcttaccgg
    27421 ccataatcgc ttcaaatatg cgggtgcggt gactactcac cagcggcagc atctcgcgac
    27481 gcgaatagag caattcgaag ttctggcgga cattctgggc caacatcggc tccatacacc
    27541 ttagcagatg aagcagaacc acattgtggg ccgcttcggt gacggcaatc tgatactgga
    27601 gtacggcgtt tgattccgcg tccagatcgc cagactgctg cgccagctct atggcgtggt
    27661 ggagttcacg gatgcgttcc ttgtcttcat cggtactacg cagcgcggcg taataagcgg
    27721 cgataccttc cagggcgtgt cgtgtttcga gcaagtcata ctgtgattca ggatggtcgg
    27781 agagcagctc caccagcgga tcgctgaagc tttgccatag gctgctctgg acaaaagtgc
    27841 cgccaccctg gcgacgaagc aacaagccct tcgcttcgag acgttgaatc gcctcacgca
    27901 aggagggacg ggagacgtcg aactgttttg ccagttcgcg ttccggtggg agtttttcgc
    27961 ccgggcggag agtgccttcg agaatcaaaa actccagttg ctgctcaatc acatcggaga
    28021 gttttggttg gcggattttg ctgtaggcca tgagttcctg tcttaagcca cttgccgaag
    28081 tcaattggtc ttaccaattt catgtctgtg acgctaaagt aacaaagtat tcaccttatg
    28141 tccatacagg ttttgattga aatcatgaaa ctgtgcacat tttaacaact tgacatatat
    28201 aacgtttcaa aattgtaact atgcacaaat gtagacttta cgtaggaaag ggatttttta
    28261 acgattatta aactataaaa atccgaattg aaccgattca cttaccaatt ttttgatttt
    28321 taagtcaata aaaacgaatt taaattcatt
//
LOCUS       DAZPDU010000039        28050 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZPDU010000039 DAZPDU010000000
VERSION     DAZPDU010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28050
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.039"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..248
                     /gene="tnpA"
                     /locus_tag="ACHURS_001534"
     CDS             <1..248
                     /gene="tnpA"
                     /locus_tag="ACHURS_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFR1237304.1"
                     /translation="MDGEPDHVHLLINYPPKLAISSLVNSLKGVSGRLLRRDRPDIAV
                     RYYYKGVLWSPGYFANSCGGAPISVIRQYIEQQQTPG"
     gene            308..382
                     /locus_tag="ACHURS_001535"
                     /pseudo
     CDS             308..382
                     /locus_tag="ACHURS_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(379..1702)
                     /locus_tag="ACHURS_001536"
                     /pseudo
     CDS             complement(379..1702)
                     /locus_tag="ACHURS_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
     gene            complement(1702..3318)
                     /locus_tag="ACHURS_001537"
     CDS             complement(1702..3318)
                     /locus_tag="ACHURS_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFR1237305.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQTGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFILGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            3614..4330
                     /locus_tag="ACHURS_001538"
     CDS             3614..4330
                     /locus_tag="ACHURS_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR1237306.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(4327..5988)
                     /locus_tag="ACHURS_001539"
     CDS             complement(4327..5988)
                     /locus_tag="ACHURS_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFR1237307.1"
                     /translation="MNDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKDTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGLLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(6184..6612)
                     /gene="ibpB"
                     /locus_tag="ACHURS_001540"
     CDS             complement(6184..6612)
                     /gene="ibpB"
                     /locus_tag="ACHURS_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFR1237308.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(6724..7137)
                     /gene="ibpA"
                     /locus_tag="ACHURS_001541"
     CDS             complement(6724..7137)
                     /gene="ibpA"
                     /locus_tag="ACHURS_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFR1237309.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(7131..7203)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            7443..7775
                     /locus_tag="ACHURS_001542"
     CDS             7443..7775
                     /locus_tag="ACHURS_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFR1237310.1"
                     /translation="MIRNVLLAFMICSGMALLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(7777..8991)
                     /locus_tag="ACHURS_001543"
     CDS             complement(7777..8991)
                     /locus_tag="ACHURS_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFR1237311.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYCASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            9092..10156
                     /gene="cbrA"
                     /locus_tag="ACHURS_001544"
     CDS             9092..10156
                     /gene="cbrA"
                     /locus_tag="ACHURS_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFR1237312.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            complement(10153..11490)
                     /locus_tag="ACHURS_001545"
     CDS             complement(10153..11490)
                     /locus_tag="ACHURS_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1237313.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(11565..12713)
                     /gene="dgoD"
                     /locus_tag="ACHURS_001546"
                     /pseudo
     CDS             complement(11565..12713)
                     /gene="dgoD"
                     /locus_tag="ACHURS_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(12710..13327)
                     /gene="dgoA"
                     /locus_tag="ACHURS_001547"
     CDS             complement(12710..13327)
                     /gene="dgoA"
                     /locus_tag="ACHURS_001547"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFR1237314.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(13311..14189)
                     /gene="dgoK"
                     /locus_tag="ACHURS_001548"
     CDS             complement(13311..14189)
                     /gene="dgoK"
                     /locus_tag="ACHURS_001548"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFR1237315.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFTAGLERGLNTPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVAHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(14186..14875)
                     /gene="dgoR"
                     /locus_tag="ACHURS_001549"
     CDS             complement(14186..14875)
                     /gene="dgoR"
                     /locus_tag="ACHURS_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFR1237316.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            15153..15809
                     /locus_tag="ACHURS_001550"
     CDS             15153..15809
                     /locus_tag="ACHURS_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1237317.1"
                     /translation="MKLNLKGFFKTAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLKDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYNWSTEEEKARVTQYRFACGVQHCTRTVENLEGTIHEKNKN
                     MDYSNVMAFYHPFKVRFYEYHSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(15855..16667)
                     /gene="yidA"
                     /locus_tag="ACHURS_001551"
     CDS             complement(15855..16667)
                     /gene="yidA"
                     /locus_tag="ACHURS_001551"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFR1237318.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(16782..17180)
                     /locus_tag="ACHURS_001552"
     CDS             complement(16782..17180)
                     /locus_tag="ACHURS_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFR1237319.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(17420..19834)
                     /gene="gyrB"
                     /locus_tag="ACHURS_001553"
     CDS             complement(17420..19834)
                     /gene="gyrB"
                     /locus_tag="ACHURS_001553"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFR1237320.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(19863..20936)
                     /gene="recF"
                     /locus_tag="ACHURS_001554"
     CDS             complement(19863..20936)
                     /gene="recF"
                     /locus_tag="ACHURS_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFR1237321.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(20936..22036)
                     /gene="dnaN"
                     /locus_tag="ACHURS_001555"
     CDS             complement(20936..22036)
                     /gene="dnaN"
                     /locus_tag="ACHURS_001555"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFR1237322.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(22041..23444)
                     /gene="dnaA"
                     /locus_tag="ACHURS_001556"
     CDS             complement(22041..23444)
                     /gene="dnaA"
                     /locus_tag="ACHURS_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFR1237323.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            23741..23821
                     /locus_tag="ACHURS_001557"
     CDS             23741..23821
                     /locus_tag="ACHURS_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1237324.1"
                     /translation="MTIDKNWLNRSNKDLGRSLRFTHQPV"
     gene            24051..24191
                     /gene="rpmH"
                     /locus_tag="ACHURS_001558"
     CDS             24051..24191
                     /gene="rpmH"
                     /locus_tag="ACHURS_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFR1237325.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            24208..24567
                     /gene="rnpA"
                     /locus_tag="ACHURS_001559"
     CDS             24208..24567
                     /gene="rnpA"
                     /locus_tag="ACHURS_001559"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFR1237326.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            24531..24788
                     /gene="yidD"
                     /locus_tag="ACHURS_001560"
     CDS             24531..24788
                     /gene="yidD"
                     /locus_tag="ACHURS_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFR1237327.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            24791..26437
                     /gene="yidC"
                     /locus_tag="ACHURS_001561"
     CDS             24791..26437
                     /gene="yidC"
                     /locus_tag="ACHURS_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFR1237328.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            26543..27907
                     /gene="mnmE"
                     /locus_tag="ACHURS_001562"
     CDS             26543..27907
                     /gene="mnmE"
                     /locus_tag="ACHURS_001562"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFR1237329.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVEVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT         6760 a         7569 c         7159 g         6562 t
ORIGIN      
        1 aaatggatgg cgaaccagat cacgtccatt tgttaattaa ctatcctccc aaactggcga
       61 tatccagtct ggtaaacagc ctcaaaggcg tatcgggtag gttactgcga cgagatcgac
      121 cagatattgc agtcaggtat tactacaaag gcgttttgtg gagtcctggc tattttgcca
      181 atagctgcgg aggtgcgcca atatccgtca tccgccaata cattgaacaa cagcaaacac
      241 ctggttaggt ggaaaaccgc gccttatatc cccgccctga gggacggggt tttacggcgc
      301 accggataaa tttggttatg cgccgtcagc cgtgctaaag aatacggacc agcacccaac
      361 ggatgccagt ccacacaatt aacgtaactc tggccagtac gctttgttgg cctccagcaa
      421 ctcatccaga atagctttgg cgaccgaggc gctcggtacc gttttcgata acgtaatcgc
      481 ctgccacagg tgctgatatg aacgctgttc ccaggcgtcc acgaccagtt tttccaccgc
      541 cacttgctga ctcattaacc ctttctgaaa ctgcgggata tcgccgacca ccagtggttc
      601 tggtccgttg tgcccgacca gacacgggat ctcgaccatc gcttcatcat caaagttatg
      661 gattgccccg ttgttaggca caatcagcaa catccgctcc tgagtgttga aggcaattgc
      721 cgccgccaga tcgacgatgt atgacgcatg ttcgtcaatc tccagcttgg ccgccgctga
      781 atttcctgcc gctgtaatgg cgcggcaagc atcgaaaacc tgtttctcgc gatgttccat
      841 tcacctcatt cgcgcgggta tgttcagggt tggaatgctg aacaacataa tccgggaaga
      901 gataatattt cagataggtg ttcggcaatg tatccgggtc cagtgcctgg acatcccgcg
      961 cttttgcata ggtgtcgttc cagctcgctt cagtatgtgt atcttgctgc ggtggaacat
     1021 aaccgtgctt cgagacatat tgccgcagct ggggcatcag gtcgttgccc tgcaaatctt
     1081 caattgatgt ccaccagcca aagtggttca ggccgtagta gcgcacgcgc atctgtttgc
     1141 gatcttgcag cccaacaatt tgcgccatcc ggctttcaat accgattggc atgtcacaga
     1201 tgttgaggat tttcgcattc gggcgcagac gacgcgtggc ttctgcgaca atggctgccg
     1261 ggttggagta gttgagcatc caggcatttg gtgaatattt ttccatataa tccaccagtt
     1321 ccaggacgcc gccaatggaa cgcatgccgt acgctattcc gccgggtccg caagtttcct
     1381 gaccaacaac accgtggcgc agtgggattt tttcatccag ttcgcgcatc gggtacttgc
     1441 cgacgcggat gtgcgccatg acaaaatcaa cgtcgctgaa cgccacttca ggatcggtgg
     1501 tgtaactaaa cgcaatgtcc ggcgctttct ctttaaggat gactttacag gcttcggcaa
     1561 tcacttcctg ccgcgcgcca tcgttatcat aaaatttcag tgcacgaagc gggaaacgct
     1621 cctgattcgc caggagcatc aacacgatgc ctggcgtaaa ggtgcttcca ccgcctgcga
     1681 caaccactga gaatttggtc attatactgc ctccgtaatg gcaacatttt ctgctgattg
     1741 atgagaatta attaagctat cgagctgttc acgcagctgg gatacatgca gaccaatgat
     1801 cacctgaatg gcatcgccac tacggaagac gccgtgcgct cccagctttt taaagacttc
     1861 gtcatccagc gtttgtgaca tgtcatgcag tgcaatgcgt agacgcgtcg cacaattgtt
     1921 aatgctggag atattgccga ccccgcccag ggcttgcagg ataccggcag cttgatccag
     1981 ctcttttttt ggctctgccg cggtggtttg gcctcgcgag gctttgtatt cggcttttga
     2041 gtagagtttc acttccgcat cttcacgtcc cggcgtgcac atgttgaact gcagaatcag
     2101 ggtgcggaaa accacgaagt acagcagggt aaagcacaac ccaatggcga tttgggtcag
     2161 catcatatcc gcgtggttgc tgaacatcgg gatccagttt tgcggtaaaa cctggtcaat
     2221 cagacctccg cccatgttgc ccaccacacc aaagagatac atcacggtcg acattgaggc
     2281 cgcaagcaca gcgtgtaccg caaacagcaa cggtgaaatg aacaggaagg taaattccag
     2341 cggttcggta attcccacca gcatggcggt taaggttgcg ggaatcagca agcccgccac
     2401 ttttacccga ttttccggtg cggcagtgaa gtacatcgct aaagaaatgc ccacggcacc
     2461 aaagattttt gagttaccgt gcagggcaaa accgccttcc gggaacaacg atttcagcgg
     2521 ctcggcgctc aaactgaact cttgcagatg ctgcgcccag tacatctgaa tgccgccttc
     2581 aacagctgcc ggacccaaga taaacggtcc gtagatgaag tggtgtaaac cggttgggat
     2641 cagaatacgt tcgaggaagg tgtaaaccca gaccccaagc gcacccgccg aacgcaggaa
     2701 cgcttgcaga gattcaatcc ccgtttgtac ttttggccag ccgagcaatg tcagccaggc
     2761 acaggggatc atcaccagga aggcgataat caccacataa gacgtcccct ggaaaatgcc
     2821 gagaaaaacc ggcagttttt tatcgaacag acggttatgc agcgccgtca caatgccgga
     2881 aatgataatt gcgccgataa tgctggtatc gagggtttta atcccggcca tcattgtcag
     2941 accgctacct gccaccgcgt cctgagtgaa atcgacgccg aagtagcttc cccaggtcat
     3001 tcccatcgcg ttgatgaaat agttccaggt caggaaactc accatcaccg ccagacaagc
     3061 acgtccctgc gcttgcttag caaggccaat gggtaaaccg acagcaaaaa tcagcggcat
     3121 attacggaat accgtccaac cgccctcttc aataatgtgt acgatttgcg cgaataaact
     3181 gttcggatcg gtcagtgatt ccccgacaaa catcgggttt tgcagcaaga tggcaagacc
     3241 caccacaatc ccggcgaagg gaaacagcag cactggcgta aacatcgcgc cgccaaagcg
     3301 ttgaatttga ctgagcattt ccacatcctc ttttctcaat tctgtaagta ggaagcctga
     3361 ttggttatgc ggcaggcctg gagcaaggat aggaagatgc gatcggcaga acatgacgac
     3421 ccaatgggat tcgtgatcgc tttcatgctt ttcgttgggc ggtagagatc tacgttttcg
     3481 cgaaaaagta gacatctgtg agcacaggta cacagcatcg aacacatctt taaaaaaaag
     3541 atgttttttc aatcgattaa gcagaacttg tgtgcgcatt acccgggctt gcaggcaaaa
     3601 aagagatcta gagatgatct acaaaagcat tgcggagcgg ttaagaattc gacttaactc
     3661 cgcagatttc acgctaaaca gccttcttcc gggtgaaaaa aagctggcgg aagagtttgc
     3721 ggtatcgcgg atgaccatcc gtaaagccat tgacctgctg gtagcgtggg ggctggtggt
     3781 ccgccgccac ggtagtggca cttacctggt gcgcaaagat gtactgcatc aaaccgccag
     3841 tctgaccggg ctggtggagg tgttaaaacg gcagggaaaa actgtcacca gccaggtgct
     3901 gatttttgaa atcatgcctg cgcctccggc cattgccagc cagttacgga ttcaaatcaa
     3961 cgagcagatc tacttctccc gtcgcgttcg ttttgtggaa gggaaaccgc tgatgctgga
     4021 agacagctat atgccggtaa aactgttccg taatctttcg ctgcaacatc tggaagggtc
     4081 gaagtttgaa tatattgaac aagagtgcgg gattttgatt ggcggtaatt atgaaagcct
     4141 gacgccggtg ctcgccgata gactgctggc gcggcaaatg aaggtagcgg aacacacgcc
     4201 actgctgcgg atcacctcgt tgtcatatag cgagagcggg gagtttttga attattcagt
     4261 gatgttcaga aatgccagcg aataccaggt ggagtaccat ttacggcgac tccacccgga
     4321 aaagagttaa ccgatactcc agaagagcac cgccagtaat tggggggtga taatgcgcag
     4381 gaacatcact aacggataga cagtggcgta agagagcgcc gccgcaccgc tagttggatg
     4441 aagattatta gcaaacgcca gcgccggagg atcggtcatg gaacctgcca gcatcccgca
     4501 catggtcagg taattcattt tggctaacat ccgcgccaga atgccaacag taatcagcgg
     4561 aacggcggtg atcagggcac cataaccaat ccagcttagc ccttcgccat tgaccagggt
     4621 attcacaaaa tccccaccag atttcagacc aacgaccgag aggaacagca cgatccccag
     4681 ctcccgcagc gcgaggttgg cacttggcgg cataaaccag tacagcttgc cgatactgcc
     4741 gatacgcccg aggatcaacg ccataatcag cagaccgccc gccagcccca gtttcaacgc
     4801 ggccgggaat cccggcacaa agacgggaat agaacctaac aatacgccaa gcccgatgcc
     4861 aataaacact ggcagcatct gaacctgttg cagtttttgc tgcgcattcc ccagcacatt
     4921 ggcaacggca tcaattgcgg acggacgccc caccaggttg aggatatcgc cgaactgcag
     4981 gctgatatcg ccactggcga ccagttcgac cccggcacgg ttcaggcgcg agataacaac
     5041 gtcatagcgt tctttaaagt gcaggtcgcg aatacgtttt ccgagcacgt tttcattggt
     5101 gaccaccaca cgctcgacgc gcaaatcagt gtctttcgtg gacagcgaag tatcgacctc
     5161 ctgaccaatc accagttgcg cattatgtaa atccgctggc tgacccacca gatgcagcaa
     5221 atcgcccagt tggataatgg tatctggcga aggaactttt agggtttctt cgcgtttcag
     5281 acgcgagcag ataattttgt cgccgttgag aatcggtaca tctttaatgg ctaaatcatg
     5341 caggttaggg ttctcaacgc gaatattgat agtcttgatc agcgcgccgc cattggtgcg
     5401 tgaagactcg tgctgctgag cttctgtctc gacattgacg cggaaaataa cccgcaacat
     5461 ccacatggtg aacaaaatcc cgcaaatgcc gaatggatac gccatcgcgt aactcatccc
     5521 catctgatcg accatttcca ttggtgtacc caggtcgcgc aaaatctgct gccctgcccc
     5581 cagcgctggc gtattggtaa ccgcaccgga gaaaatcccc agcactaccg gcagtggaat
     5641 atcaaacagt ttatgcagga tggcggtaac cagaccaccg atgatgacga tcagaacagc
     5701 aaacaggttg aggcgtaatc cggagacgcg caatgaggca aagaagcccg gccctacctg
     5761 aatcccgatg gtataaacga acaggatcag gccaaattcc tgaataacat gcagcatatc
     5821 gctacttaat gtcatccccg cctgagaaac aaaatggccg acgatgatcc caccaaacag
     5881 cacgccgcca atacctaatc ctatgccgcg aaatttgacg ttgccgataa acaaaccgac
     5941 gactgccacc aaagccagaa tactgaccgt taatgctata tcattcatcg tccatttctc
     6001 ttgagataac attttaattg ctacggatta tggctgaatc ggtgaagcag atttgcctta
     6061 tagcgcacat tattacggga aagaacgtgc cgaaatatct taaacagtcg aaatttaacg
     6121 cattgttgaa gaagaaagac aaaaaaagcc ccaccagaat ggcggggcaa agagaatagc
     6181 tagttagcta tttaacgcgg gacgttcgct gatagcgata cgctgcgctg cgatgggttc
     6241 aggctcatta cgaattaaat caatatgcag taaaccgttt acgaaagttg cgccagagac
     6301 ttccatattt tcagccagcg taaagctcag gctaaatggc tgattcataa gcccttgatg
     6361 cagccatttt ttctcttctt ttggctgctc cggcgtgcct tttacgctca ggcgcgtacc
     6421 ttccagttga atctctaaat cttcctgacg gaaacctgcc agcgcaaggg taatgcggta
     6481 gtggttatcg tcgcttttct caatgttgta cggcgggaag ctctgacttt caccggcgtt
     6541 ttgcagtgcg ttggccagtt tgtcaaaacc gatccattga cgcatcagtg gggataaatc
     6601 gaagttacgc atagtcattt ctccttctaa gaagcgagta agtacctgca aatccgaaga
     6661 ttcgcatatg ctccctgacg gcgagcatgg agatgtcagg ccgcgccagg cggccttaag
     6721 gaattagttg atttcgatac ggcgcggttt tttcgcttcc ggaatcacgc gttcgagatc
     6781 gatatacagc aaaccattta ccaggttagc gccacgaaca tgaatgttct cagctaactg
     6841 gaatttgcgt tcaaagttgc gttcagcgat gccctggtac agataggtgc gctctttttg
     6901 ttcgtcggcg tgagcacctt tcaccaccag cagattatcc tgggcggtaa tttccagttc
     6961 gctctcagca aaaccagcca cagcgatagc aatgcggtaa tggttttcgt ctaccagttc
     7021 aacgttatac ggagggtagc cgccattact ctggctctgg ttgttttcta agtggttaaa
     7081 caaacggtca aatccaatag cagaacggta aagcggggat aaatcaaagt tacgcataat
     7141 caatagctcc tgaaatcagc gagaatgtaa gaccttccac aatggacagg tcaggtagcc
     7201 agaacaccca tcaggcgcgt cctcatcgac tacgatgtaa aaatgggtct ggaaatgaac
     7261 ttttcaagcc ttatcagcgg actttttttt gcagtttatg gtctattgca tagactgagg
     7321 gggcagcacg aaacgttaaa gtgcgatagc cgccacagag cgacgtaatg atgatgttat
     7381 tttcagcaag ggatcgctat aactcatcag gccaacaccc acgatcaaca gatgaattta
     7441 ttatgataag aaatgtgttg ttagcgttca tgatatgcag cggaatggca ttactcggag
     7501 gatgctccag cgtgatgtcc cataccggag ggaaagaagg aacgtatccc ggcacccgcg
     7561 ccagcgccac catgattggc gatgatgaaa ccaactgggg cactaagtcg ctggcgattc
     7621 tcgatatgcc gtttaccgct gtgatggaca cgcttttatt accgtgggat gtgttccgca
     7681 aagacagctc ggtgcgctca cgcgttgaaa aaagcgaagc caacgcgcaa gccaccaatg
     7741 cggttattcc accggccaga atgcctgata actgatttag cgatcagttt cggtgatcca
     7801 cagctggcca ccttccatcc acgccagcca ttgaccatcc ggcgagaaga ccacggcgtc
     7861 cgcagaaggt ggattggcgt ggttttcggt taaatactca acctcgccgc tttgcgcatg
     7921 ggcacaagca attcgattat ccagcacaaa gcccaaccat tctcctgacg gatgccagtt
     7981 aaatgcagac tgaatatccg ttttgttatg ggttaactgg cgcggctcgc cgccctgtgg
     8041 cgagataagc cacagttgca caatgccgtt atcatcacgc attaaaaacg cgatttgcgt
     8101 accctgcgga ttacagcgca cccagtggcg cgggacgttg actaaccccg gataagcccg
     8161 atggtgggta aaggttaaac gtcgctgcac gacgccacgc ggtggcgcgg gcagggtggt
     8221 ttccgttcca cttaacggcg catcacctgc cgctttccag ccagcttcat cttgcggcaa
     8281 ctcaacgata aacagctccg gcactttctc accctttggc gaaagtgtgt cgccaataaa
     8341 cgccagcgcg tgatttccta cccatccttc ttcataagca cgattgattt catcactgcc
     8401 aggctgcggc gtgggcgtgg ttttactcac cagcacacac cagtggctgc cgctgtattc
     8461 acgcggatgc tgcttttgta cgttgaccgg accaaacggc gcagcaacac cgacgtttcg
     8521 caaatccagc gccggatcga gttcatgcat tacatggtcg ttataggtaa agctcaccct
     8581 ttcaccgttc gggctaaaga catgcacatg gctgccgccg cgcagcgctc ctggcgtata
     8641 cggcgcggta atatccattg catcgagatt gctcaccttg ccgccttcag caatgactcc
     8701 gcgccgatga tggaagtcgt aatgccatgt ttcatcagga ttttccgggc cgtgaataaa
     8761 gacatatttc tctgacttcg gatgaacggt caccacgccg acgtgtgcgc cctgtgacgc
     8821 acagtagata acctcgacct caccggtatg gatattcaca cgctcaatgg tctcgccggt
     8881 aaacgacgcc ccagaaggac gcacgtcaaa taccagccac tggctgtcgg gcgtccaggt
     8941 attggtattg gtgagcaggt gattacgggg agcaaaggtt atctgtttca tggcgcggtc
     9001 ctgatacaaa acgggtccag ccatcatact tcacaaggac ttcactttca gacaatagtc
     9061 ttttgccatc gtgatgaatg tgagtgaaaa gatggaacat ttcgacgtgg cgattattgg
     9121 cctcggcccg gcagggtcgg cgttggcacg aaagttagcc ggcaaaatgc aggtgatcgc
     9181 gctggataaa aagcaccagc atggtactga aggtttcagc aaaccttgtg gcggtctgct
     9241 ggcaccggac gcgcagcgat cttttattcg cgatgggctg acgcttcctg tcgatgtgat
     9301 cgctaatccg cagattttca gcgtcaaaac cgtcgacgtc gccgcatcgc tcacgcgtaa
     9361 ctaccagcga agctatatca atattaatcg ccatgctttc gacttgtgga tgaaatcgct
     9421 gatccccgcc agcgttgagg tttatcacga cagcctgtgt cgaaaaatct ggcgtgagga
     9481 tgataaatgg catgtcattt ttcgtgcaga cggctgggag cagcatatta ccgcccgcta
     9541 tctggtcggt gccgatggtg ccaactcgat ggtgcggcga catctctacc cggatcatca
     9601 aatccgtaaa tatgtcgcaa tccagcagtg gttcgcagag aaacatccgg tgccgttcta
     9661 ctcctgcatc tttgataatg cgataactga ctgttattca tggagtatca gcaaagacgg
     9721 ttattttatc tttggcggtg cctatccaat gaaagacggt cagacgcgtt tcacgactct
     9781 gaaagagaaa atgagcgcct ttcagttcca gtttggcaaa gcggtgaaaa gcgaaaaatg
     9841 cacggtgctg tttccctcac gctggcagga ttttgtctgc ggtaaggaca acgcctttct
     9901 gattggtgaa gcggcgggat ttatcagcgc cagctcgctg gaggggatta gctatgcgct
     9961 ggatagcgca gacattctgc gttcggtgtt actgaagcag ccagagaagc tcaataccgc
    10021 ttactggcgc gccacccgca aactgcgttt aaaactcttc ggcaagatag taaaaagccg
    10081 atgcctgacc gcaccggctt taagaaagtg gattatgcgc agtggtgtgg cacatattcc
    10141 acagttgaag gattagccaa cgcgcttcac atcgcccacc agcaggatat aagagagcgc
    10201 gccaatcaac gcgacggcgg agatataaac cagtgcaggt gcgaaaccgt aaccctgcgc
    10261 caggtacccc accaccagcg gaacggtgat gccgcccaga ccaccggcga agttaaacac
    10321 gccgccggtt aaaccaatca ggcgcatcgg tgccagagaa gaaaccagcg accaggtaat
    10381 cgaagcaaaa ccgttaccga agaatgccag cgccatcagg cacataatca tcatcggatc
    10441 gttagtgtag ttagcgccca taatgcaggt ggagatcagc aagccgcaga taatcggcgt
    10501 tttacgcgca aagcccagtg aaaagccctt acgtaccagc agatccgcta cccagccaga
    10561 gagcaggacg ccgacaaacg ccgcgaggaa tggcaccgtg gtcataaagc ctgctttcag
    10621 cgccgtgatt cctttttcct gggttaaata gttcgggaac caggttaaga aaaaccacag
    10681 tgtagaagcc accgcaaatt gcccaagata gacgccgatc agtttacgat ggaacaccag
    10741 tttccagtct ttggctgtta acggctgacg tgcctctttc ttcactggcg catcaccatc
    10801 caccagaccg ccgccatcac gaatgtaatc cagttcagct ttgctgatgc ctttggtcag
    10861 gcgcggcggc tgataaacct taaaccaaat cagcgaccag ataatgccga taccaccagt
    10921 cacaatgaac acccagtgcc agctcaacat ctcctgaatc cagatcagca gcggagtcaa
    10981 aaacgccaga ccgacaaact gaccagacgt ataaaaacca acggcggaag cgcgttcatg
    11041 ttccgggaac cagctggtca ccatccggtt attggtcggg aaagcgggcg cttcgaaaat
    11101 accggttatc gcacgcagac caattaatga cattaatccc gtggcaaagc cctggaataa
    11161 agtcgccact gaccagccaa atatcgcaat aaaataagtc acgcgagaac ctacgcgatc
    11221 taaaaaccaa ccgccgggga tctggcatag cgtataaagc caggcgaagg ccgaaaatac
    11281 atagcccatt tccgctttcg taatgccgaa ctcttcctga atatgggcgg aagccacggc
    11341 caggttagcg cggtcaacat aacaaatgac caccgtaata aagatcatca ccagcgtcag
    11401 ataacgccga cgccccggct ttgctgcatt aacgggaata tccatagcga gctttctcca
    11461 gattttgggc atagcgaagc cgctcaccat gccctgtaat ttacagaggg ttatattttt
    11521 gtattgctgt tttagtgccc gatgaggggc ttacgtggca ggaattacca ctctgctacg
    11581 ctgttatctt catgacgcca gagtggatta cgccagtccg gggcattttt actgaactca
    11641 ataactttag cttcgtcgat ttccacgcct aagcccggtt tcgttagcgg tttaaagaag
    11701 ccgccaacca tgctgaagtc ttctttgttt ttcacaaagt cgagtaactc cgcgcctttg
    11761 ttgtaatgaa ttcccatact ttgttcctga agtaccgcgt tataggaaac aaagtcgata
    11821 tgcagacaag ccgccagtgc aatcggtccg agcggacagt gcggcgcaag ggtcacgtca
    11881 taggcttctg ccattccggc aattttgtag cattcggtaa taccgcctgc atgggagaga
    11941 tccggttgta gaatcgaaat accgcctgcc tccagcacgc gtttaaaatc gaaacgtgag
    12001 aacatgcgct cacccgccgc cagtggaata tgcgtttgtg ccgccaattt cgggtagtat
    12061 tcggcttgtt ccgccagcac cggctcttca ataaacagcg ggcgatacgg ctccagttct
    12121 ttaatcagca ctttcgccat tggcgcgctg acgcgaccgt ggaaatcaag accaaactca
    12181 atctgattgc caaaagcttc acgaatttgt gccacggtgt tgactgccgc atctaccgcg
    12241 cgggagttat caattagccc cagttcttca caaccgttca gtttgaaggt atcgaagccg
    12301 atttcgcgca gggttttaat accgtcgatc acatccgccg gacgatcgcc gccgacccaa
    12361 ctgtaggctt taattttgtc gcgaaccagg ccgcccatca gttgccagac cgacgcattc
    12421 agcactttac ctttgatatc ccataacgcc tggtcgatcc cggcaatggc gctcatcagg
    12481 attggaccgc cacgataaaa tccggcgcga tacatcactt gccataagtc attgatgcgc
    12541 tatggatcct gaccaatcaa atagtcaccc agctcgtgaa ctgccgcttc caccgtgcgg
    12601 gcgcggcctt caatcactgg ctcgccccag ccgacgacgc cttcatcggt ttcaattttc
    12661 aggaacatcc agcggggagg taaacgatac gtggtaattt tggtgatttt cattgcactg
    12721 cctctcgata cgccttaaca aatgctgctg cctgctgcgc ggtgcgttct acggactgcc
    12781 cggcgcgata gagatcgctg cctaagcccg ccccagcaca acctgcgtct atccactgcg
    12841 ccaggttttc tggcgtcacg ccgccaacgg caaagactgc gatgtccgat ggcaataccg
    12901 cttttaacgc tttgatgtat tgcggaccaa aagccgatga cggaaatatt ttcagcgcct
    12961 gcgcgcccgc ttcgagcgcg gtaaaggctt cggtcgccgt cgcgcagccg gggcagacgg
    13021 tcatgccgta gcccaccgca cggcggatca cttcactatg gatattgggc gtaacgatga
    13081 gctgacagcc catcctggcg agcgcatcga cctgttcagg tttcagtacc gtacctgcgc
    13141 caatcaacgc cttgtcgcca tacgcatcaa cgatggcggg aatgctttgc tcccattgtg
    13201 gggaattcag cgggatttca actgcgtcga acccggcgtc aatcaccgcg ccaacatgcg
    13261 ccagcgcctc gtcgggctta atgccgcgca aaatggcgat cagcggaagt ttagtttgcc
    13321 actgcatgag cgatgctcct tataccagcc tgaaatgccg tgtcgcccgc caccgccgtc
    13381 acgtcgcaac ccatcgcctg aaaggcttgc tggtagcgcg cggtcaacga tgttccggcg
    13441 acaagggtga tggcgtgttg atgggccaca tagtcgcgca tactggcgac ctctgcgcca
    13501 atcaacaaac cagagagaaa ttcactgacc tgttcacggg gaagtgttcc cagcacatgc
    13561 gaggcacgaa cttcaaaaag ctgcggcaat atggcgggcg tattaagacc acgctcaagg
    13621 ccagccgtga aggcatcggc agagttttcc tgcggcggca aacctgcacc aatcaatgag
    13681 tgatttaaca gtaaatgatg taattcaccg gtcatcacgg tgcgaaaatc gttgatttgc
    13741 tggctatcgg cctgcaccca tttgcaatgc gtaccgggca tgacataaag agaggaagga
    13801 gccagagctc gcgcgccgat caattgagtt tcttcgccgc gcatcacatt gtggttatca
    13861 tcatgagaga cgcataatcc gggaataatc cagatattgt cgccaacaga cgttaattgt
    13921 tcgccaatag acgaaaaacg ggcaggaaca gataaatacg gagcaacttt ccagccgaca
    13981 ttgctgccga ccattcctgc cattaccact ggcgtattct cttcacgcca gtcggtcgtg
    14041 acttctgcta acaccgcagc cggagatttt ccgttcaggc gcgtgacgcc tgcttctgat
    14101 tgcctgctct ccaggcagtg gtcgccctga taaagccagg cgcgcagatt ggtcgatccc
    14161 cagtcaattg cgatgtagcg agctgtcatg tgatttcctt taaccttcgt gtcgagctgg
    14221 cgatcatggt aagcgccgcc tgctctgccg catcgccgtc ctgatgccgt atcgcatcga
    14281 acagcgcctt atgttcctgg agcgtttgcg gcatgttggc ctcatcgccc atccaggttc
    14341 gttcaaaaac cgcccgctgc agcgaactga tcgcaatgct aagttgctgt aacaccgggt
    14401 tatgcaccga ctgcagcacc gcctcgtggt agcgaatatc cgcttcgtta aacgcttcgc
    14461 ggtcctgatt gttggcaatc atctcgttca gcgccgattc aatctgcgcc agatcgctgg
    14521 aggtcgcgcg ctctgctgcc caacgggcaa tcgccggttc caccagattt cgcacttcgc
    14581 tcatggcact gataagccgt gggtcgtagt cgttttccag cacccattgc agtacgtcag
    14641 tgtcgaggta attccactgg ttacgcggtg ccacaaacgc gccgcgataa cgtttcattt
    14701 caatcagccg tttcgccatc aacgaacgga acacctcacg gatgatgttg cgcgaggttg
    14761 caaactcctc acagagttcc gcctcagccg gaagcggcga gcctggtacg tatttgccgt
    14821 gaacgatctg tttacccagc gtaatgacga tgcgatcggt tttattgaga gtcatggaga
    14881 gtccttgtgc tctgtatgtt cttctctact ttaccccgat cgatgcataa cacggcaact
    14941 ttgtagtacc agcgtgatga cgttcgcgtt tgctgtgcgt gtaatgtagt acaaacttat
    15001 attgttgtac tacaatttag atcacaaaaa gaacaatgcg taaaaaaatg acatgcgtcg
    15061 ggcagaaatc tgaaaaggga tatcaggcgc taaacaggag ggaaagaaga gtatgctttc
    15121 aacggcttag ctactcgttt aaaggattaa tcatgaagtt gaatcttaag ggatttttta
    15181 agactgccgg tttattccca ctggcgctga tgctttcggg ctgtatctcg tatgccctgg
    15241 tttcccatac cgcaaagggt agttcaggaa agtatcaatc gcagtcagac accatcacgg
    15301 ggctatcgca ggcaaaggat agtaatggaa caaaaggcta tgtttttgta ggggaatcgc
    15361 tggattacct tatcacagat ggtgccgatg acatcgttaa gatgctcaag gatccagctc
    15421 ttaaccggca caatattcag gttgctgatg acgcaagatt tgttttaaat gcggggaaaa
    15481 agaaatttac cggcacaata tcactctatt acaactggag cacagaagaa gaaaaagcac
    15541 gagtgacaca atatcgtttt gcttgtggtg ttcagcactg tacccggaca gtagaaaacc
    15601 tggaaggtac tatccatgag aaaaataaaa acatggatta ctcaaacgta atggctttct
    15661 accatccatt taaagtgcga ttttatgaat accattcacc cagaggcatt ccggatggtg
    15721 tttccgcagc attactgcca gtgactgtta cgctggacat cattactgca ccgctgcaat
    15781 ttctggttgt atatgcagta aaccaataat cagtaagcgg gcaaacgcgt ttatgctgtt
    15841 tgcccgccca cagattaatt cagcacatac ttctcaatag caaacgccac gccatcttca
    15901 aggttagatt tggtgacaaa gttcgccact tctttcaccg aaggaatagc gttatccatc
    15961 gccacaccga cgcctgcata ttcaatcatt gcgatgtcgt tttcctgatc gccaatcgcc
    16021 atgatttctt ccggtttaat acctaacacg tcggccagtg atttcacacc cgtacctttg
    16081 ttaacgcgtt tatcgaggat ttcgaggaag tacggcgcac ttttcagcac ggtatatttc
    16141 tctttcactt cctgcggaat acgcgcgata gcctggtcga ggatggcggg ttcatcaatc
    16201 atcatcactt tcaggaactg ggtattgggg tccattttct ccgcttcgca gaacaccagc
    16261 ggaatagtgg caacgaaaga ttcatgcact gtgtagtagc tgatatcgcg gttggcggtg
    16321 tacagcgtgg tgcggtccag ggcgtggaaa tgagaaccga cttcgcgaga gagtttttcc
    16381 aggaaacgat agtcgtcata gctgagagca gtttgcgcca cggtgctacc atcagcggcc
    16441 ttctgtacta gcgcgccgtt ataagtaatg cagtagtcgc ccggctgttc catatgcagc
    16501 tctttcaggt agttgtgcac acctgcatac gggcgacccg tcgttagcac gacattcacg
    16561 ccacgggcgc gagctgcggc aatcgcattt ttaacggcgg gtgaaatggt gtgatcgggc
    16621 agcagaaggg tgccatccat atcgatagca atgagtttga tagccatgag ttccccaggt
    16681 agattggttc ctgacccatg ctaacgcgat tccgctcaaa aatcagtaca acacccgagg
    16741 gaaaaggggg atgcaacgcg ggttgcatgc tccccatttg cttagcggaa gagtttccct
    16801 ttcagcagtt ccatacctgc ggaaagcaga tcgttattgg cttgtggtga cacttcacct
    16861 tgcggtgaga gcgcatcaat aatcttcggc aattgctctg ccagtaaact ggaagctgta
    16921 ctggtatcca cgccaagttt ttgcccgaga tcggacaccg catttgtgcc gagcgccgat
    16981 tccagttgct cgccactaac cgattgattg ccctgttgat tactcagcca ggttgagaga
    17041 atggccccta agccgccact ttgcagtttt tccagcagca cctgaatgcc gccctgctcc
    17101 tcaacccaac ttaaaatagc ctgatatttc cccgcatcgc ctttcagaaa ggcaccgaca
    17161 acttcatcaa aaagccccat gataatcacc tgtaaagcgt tacgtgttga cccaaaaagt
    17221 atagatttgc gggatgataa ttgcggattg cagaaataaa aagggcggag atgatctccg
    17281 cccttttctt atagcttctt gccggatgcg gcgtgaacgc cttatccggc ctacaaaatc
    17341 atgaaaattc aatacattgc aagattttcg taggcctgat aagcgtagcg catcaggcac
    17401 gctcgcatgg ttagcgccat taaatatcga tattcgccgc tttcagggcg ttctcttcaa
    17461 taaacgcacg gcgcggttca acggcgtcgc ccatcagcgt ggtgaacaac tggtcggcag
    17521 caatcgcatc tttaacggta acgcgcagca tacgacggct ttccgggtcc atggtggttt
    17581 cccacagctg ttccgggttc atctcgccca gacctttata acgctggatg gagaggccgc
    17641 gacgggactc tttcaccagc cagtccagcg cctgctcgaa gctggctacc ggctgacgac
    17701 gctcgccacg ttcgataaac gcatcttctt ccagcaagcc acgcagtttc tcacccagcg
    17761 tgcagatacg acgatattcg ccaccggtga taaactcgtg atccagcgga tagtcagtat
    17821 ccacaccgtg ggtacgcacg cgaacaatcg gttcaaacag gttttgttcg gcattggtgt
    17881 ggacatcaaa cttccactgg ctgccgtgct gttctttgtc gttcagttcg ctgaccagcg
    17941 cgttcaccca gcgggtaacg gtctgctcat cagagaggtc ggcttccgtc agcgtcggct
    18001 gatagataag ctctttcagc attgctttcg gataacgacg ctccatgcgg ttgatcattt
    18061 tctgcgtcgc gttgtactca gacaccaatt tctctaacgc ttcgccagcc aatgccggtg
    18121 cgctggcgtt ggtgtgcagc gttgcgccat ccagcgcgat agagatctgg tactgatcca
    18181 tcgcttcgtc gtctttaatg tactgttcct gcttgccttt cttcactttg tacagcggcg
    18241 gctgagcgat gtagacgtga ccgcgttcaa cgatttccgg catctgacga tagaagaagg
    18301 tcaacagcag cgtacgaatg tgcgagccgt cgacgtccgc atcggtcatg atgatgatgc
    18361 tgtgataacg cagtttgtcc gggttgtact cgtcacgacc gataccacag ccaagcgcgg
    18421 tgataagcgt cgccacttcc tgagaagaga gcatcttatc gaagcgcgct ttctcaacgt
    18481 tgaggatttt acccttcagc ggcagaatcg cctggttctt gcggttacgc ccttgcttcg
    18541 cagagccgcc cgcggagtcc ccttccacca ggtacagttc ggaaagcgcc ggatcgcgtt
    18601 cctggcagtc tgccagtttg cccggcaggc ccgctaagtc gagcgcacct ttacggcggg
    18661 tcatttcacg cgcgcgacgc gccgcttcac gggcacgggc agcatcgata attttgccaa
    18721 ccacgatttt cgcgtcggtt gggttttcca gcaggtattc tgccagcagt tcgttcatct
    18781 gctgttcaac cgctgatttc acctcagaag aaaccagttt gtctttggtc tgggaggaga
    18841 atttcgggtc cggcactttc acggaaacga ccgcaatcag gccttcacgc gcatcgtcac
    18901 cggtggcgct aactttggct tttttgctgt agccttcttt gtccatgtag gcgttcaggg
    18961 tacgggtcat cgccgcacgg aagcctgcca ggtgagtacc gccgtcacgc tgcggaatgt
    19021 tgttggtaaa gcagtagatg ttttcctgga agccatcgtt ccactgcaac gccacttcga
    19081 cgccaatacc gtctttttca gtggagaagt agaagatatt cgggtggatc ggcgttttgt
    19141 tcttgttcag atattcaacg aacgccttga tgccgccttc atagtggaag tggtcttctt
    19201 tgccgtcgcg cttgtcgcgc agacgaatgg aaacgccgga gttgaggaac gacaactcac
    19261 gcagacgttt cgccagaatt tcatattcga actcggtcac attggtgaag gtttcgaggc
    19321 tgggccagaa acgcaccatg gtgccggttt tttcagtctc gccggtaacc gccaacggtg
    19381 cctgcggtac accgtgttcg tagatctgac ggtgaatttt accctcgcgc tggataacca
    19441 gctccagttt ttgcgacagg gcgtttacta ccgaaacacc aacgccgtgc agaccgccgg
    19501 acactttata ggagttatcg tcaaatttac cgcctgcgtg cagaacggtc atgatcactt
    19561 ccgccgccga tacgccctct tccgggtgaa taccggtcgg aatgccgcgc ccgtcatcct
    19621 gtacagagac agagttatcg gcgtgaatgg tgacgataat ttctttacag tgacccgcga
    19681 gcgcttcgtc gatagcgtta tctaccacct cgaataccat gtggtgcaga ccggtgccgt
    19741 catccgtgtc gccgatatac atacccgggc gcttacgcac cgcatccagc cctttcagga
    19801 ctttgatact ggaggagtca taagaattcg acatcaacgt ttctcgctca tttatacttg
    19861 ggttaatccg ttattttacc cttttccacg gtaaacatct tcgaattttc gtccgacatg
    19921 tctataacgt gttcagcact gatcgcgctg acaaagacct gtgattgcgt cgcttttaag
    19981 cggctggcaa gcagcccgcg acgctcatca tcaagctcag aggcaaaatc atctatcagg
    20041 tagagacacc gccgcccgct ttcacgggtg aggaactctc cttgcgccag acgtaaggcg
    20101 cacatcaaca gcttaagctg cccacgcgat aaggtatctt ccaccggcgc accgtcggcg
    20161 cgaatgcgta agtccgcttt atgcgggcca tgcgcggtgt aggttagctg gcgatcgcgt
    20221 tcaaaattac gttccagcac ctcagcatat tctgtctctt tctcccagcc gcgctggaaa
    20281 gagaaagtca gagaaaactc agggagaaat tgcttacaag tatcggccat atcagccgcg
    20341 ataccggcgc tatactccgc gcgccaggtg ctgatttgct ccgccagcgg gatcagctct
    20401 ttatcccacg ggcgtagctg ttcgtaacgt gtcacctggc gcagcgccgc attgcgctgc
    20461 ttgagcaatc gcttgagatt gctccaggcg gtgaaaaatc cgggttcgtt gtgaaagcat
    20521 ccccagtcga ggaatgctct tctgtatttg gggccgccgt tgagtaaagt aaacccttct
    20581 ggcgttatca actgcattgg catcaggtgc gccagttccg cgaccttatg cccgtctgta
    20641 ccgtcgatgc ggactttgct gtcgccctgt ttgtctttgg ttaagccaat cgctgtctcg
    20701 cgctcttcgc cctgtaatcg cccgtggaga acaaacgcct cctgctcatg gcgaatgacg
    20761 cgaccaatct gcaaactgcg aaacgcccga ccatggccga gcgtatagat ggcttccagc
    20821 acgctggttt tgccactgcc gttggcacct accagaaagt taaagccggg agataaagcg
    20881 agatccgcgg tttcaatgtt gcggaaatcg cggatcaaca agcgggtgag ggacattaca
    20941 gtctcattgg catgacaaca taggcagccg actgtgatgc ggcatcttca atctgcacgc
    21001 tggaaaccga atcggtcagc atcatgcgga cgttttcaca tttcagcgcg ttcagaacat
    21061 ccagcacgta gctgacgttg aagccgattt ccatctccgc accgctatag gtaacgtcga
    21121 ggatctcttc cgcttcttcc tgttccgggt tgttggcggt gattttcagc tggttttcgc
    21181 tgacataaag acgtacgccg cggaatttct cgttagagag aatcgccgcg cgagcaaacg
    21241 cctgcttgag cagatcgcag ccagcttcca gatgtttgtc cgggttcttc ggcagaacgc
    21301 ggcgataatc cgggaagcga ccatccacca gtttggaggt gaagataaag tcgccaacgt
    21361 gggcgcgaat gttgttgctg ccaatctgta cgcgcagcgg attgtcgccg ccgtcgagca
    21421 tacgcatcag ttcaatcacg cctttacgcg gcacgatcac cgaatggctt ggcaaagatt
    21481 gaccaattgg cattgaacag actgccagac ggtggccgtc ggtcgccacg gtgcgcagtt
    21541 cttcgccttc ggtttcaaac agcataccgt ttaagtaata gcgaacgtcc tgatgcgcca
    21601 tagaaaactg ggtcgcttca atcagacgct tcatcgtcgc ctgcggcagg gtaaattcga
    21661 cttcgctctg ccagtcatcg aggttcggga aatccgccgc tggcagggta gacagcgaaa
    21721 aacggctacg cccggagcgc accagcatcc gttcaccttc cagctgtacg gcaatttccg
    21781 cgccttcagg cagaccacgg cagatatcaa agaatttgcg cgccggaacg gtcgtcgctc
    21841 ctggctcgtg tggctgaacc agcgcaacgc gtgccaccat ttccatctcg agatcagtac
    21901 cggtcagcga caacgtaccg tcagcaacct gtaacagcag attaccgaga atcggtagcg
    21961 taggacgacc acctaacggc ccgctcacct gttgtagcgg ttttaataaa tgctcacgtt
    22021 ctacggtaaa tttcataggt ttacgatgac aatgttctga ttaaatttga aaaatcttct
    22081 ttgatatcgt ggctctcttc acgcaactgc tcgatcttac ggcaggcatg aagcaccgtc
    22141 gtgtggtcac gaccaccaaa cgcatcgcca atctccggca gactgtggtt agtcagctct
    22201 ttcgccagcg ccatcgccat ctggcgcgga cgcgccaccg agcgggatcg acgcttggaa
    22261 aggagatccg cgactttgat cttgtagtac tccgccaccg tcttctgaat attgtcgatg
    22321 gtgaccagtt tttcctgcaa tgccagcaag tcgcgcagtg cctcacgcac gaagtcgatg
    22381 gtgatcgccc gtccggtaaa gttggcgtta gcaataacgc ggttcagcgc cccttccagc
    22441 tcacgtacgt tagatcgtag acgcttggcg ataaagaacg ccacttcgcc tggcaaacga
    22501 atgtcatttt cgtcggcctt tttcatcagg atcgccacgc gggtttccag ctctggcggt
    22561 tcgatcgcca cagtcagtcc ccaaccgaag cgggatttca aacgatcctc aacgccgttg
    22621 atctctttcg gatagcgatc cgaggtgaga atgatctgtt gattaccttc cagcagggcg
    22681 ttgaaggtgt ggaaaaactc ttcctgagat cgttctttat tagcaaaaaa ctgaatatcg
    22741 tcgatcagca gtgcatctac ggaacggtag tagcgtttaa actcttcgat cgcgttgttt
    22801 tgcagggctt taaccatgtc ctgaacaaag cgctcggagt gcatataaac cactttggca
    22861 ttcggcttgc gcgccataat gccgttaccc accgcatgca gcaggtgagt tttacccaga
    22921 cccgtgccgc cataaaggaa caatgggtta taggcaccgc cagggttatc cgccacctgg
    22981 cgagccgccg cgcgcgccag ttggttagat ttaccttcaa cgaagttatc aaacgtgtgt
    23041 ttgacgttta cgttagaacg ataggtcggt tctgccgggg ccgggacgtt atcccaacct
    23101 gagcgcgtag aaggcgcagc acgttgcggc tgcgtttgcg ccacctgtgc aggggccgcg
    23161 acgttgctcg tcactgccgc ttgtggcgtt tgcgtcaccg gtttggtgcc gacttcaaaa
    23221 cgcagctgtg gggcatccgc tccgcagaaa ctggttagca gtccattgat attattaagg
    23281 tacttgtccc gtacccaatc gaggacaaaa cggtttggcg cgtacagggc cagcgtgtta
    23341 tcgctcagtt ccgcctgcaa tgggcgtatc cacatactga attctgtggc tggtaactca
    23401 tcctgcaatc gggcaagaca ctgctgccaa agcgaaagtg acacggcgga ctccactcga
    23461 acaaaagtcg ataatgacta aggctgaaac attcatgatt gttgacgtac gtcgaaaaga
    23521 ccctgcttga gggtgacgca cgaaccgctg tctgcggtta tatgcccgat caagatcctg
    23581 caaaacgatc gggaccgcgg atcatagcct aaactgcgca agagatcttc tgtttctcac
    23641 agattttccc gatttatcca caggactttc cagacctcgc taagtgtaaa cgatcctgcc
    23701 gcgaggcggg cacgatttac gccgcatatt ggaaaattta atgaccatag acaaaaattg
    23761 gcttaatcga tctaataaag atctaggacg atccttgcgc tttacccatc agcccgtata
    23821 atcctccacc cggcgcgcca tgctggtttt cactggtgtg aggtcgtaca ttttccctgc
    23881 gaaaaggtgc ggaaaagcgc ggtaaataag gaaagagaat tgactccgga gtgtacaatt
    23941 attacaatcc ggcctcttta atcacccatg gcttcggtgt ccatcgtttc atttttcggc
    24001 ggatatccaa taaagccatt gaatttattc aagtttaggt agaaatcgcc atgaaacgca
    24061 cttttcaacc gtctgtactg aagcgcaacc gttctcacgg cttccgtgct cgtatggcta
    24121 ctaaaaatgg tcgtcaggtt ctggcacgtc gtcgtgctaa aggccgcgct cgtctgaccg
    24181 tttctaagta ataaagctaa cccctgagtg gttaagctcg catttcccag ggagttacgc
    24241 ttgttaactc ccagtcaatt cacattcgtc ttccagcagc cacaacgggc tggcacgccg
    24301 caaattacca ttctcggccg cctgaattcg ctggggcatc cccgtatcgg tcttacagtc
    24361 gccaagaaaa acgttcgacg cgcccatgaa cgcaatcgga ttaaacgtct gacgcgtgaa
    24421 agcttccgtc tgcgccaaca tgaactcccg gctatggatt tcgtggtggt ggcgaaaaaa
    24481 ggggttgccg acctcgataa ccgtgctctc tcggaagcgt tggaaaaatt atggcgccgc
    24541 cactgtcgcc tggctcgcgg gtcctgatag ccctcattcg ggtctatcaa cgcctgatta
    24601 gtccgctact cgggccgcat tgtcgtttca ctccaacctg ttcaagctac ggaattgagg
    24661 cattgcgcag gtttggagtg ataaaaggca gttggttgac ggtgaaacgc gtattaaaat
    24721 gccacccttt acaccctggt ggtgacgatc ccgtcccgcc cggaccattt gataccagag
    24781 aacactaacg atggattcgc aacgcaatct tttagtcatc gctttgctgt tcgtgtcttt
    24841 catgatctgg caagcctggg agcaggataa aaacccgcaa cctcaggccc aacagaccac
    24901 gcagacaacg accaccgcag cgggtagcgc cgccgaccag ggcgtaccgg ccagtggcca
    24961 ggggaaactg atctcggtta agaccgacgt gcttgatctg accatcaaca cccgtggtgg
    25021 tgatgttgag caagctctgc tgcctgctta cccgaaagag ctgaactcta cccagccgtt
    25081 ccagctgctg gaaacttcac cgcagtttat ttatcaggca cagagcggtc tgaccggtcg
    25141 tgatggcccg gataacccgg ctaacggccc gcgtccgctg tataacgttg aaaaagacgc
    25201 ttatgtgctg gctgaaggtc aaaacgaact gcaggtgccg atgacgtata ccgacgcggc
    25261 aggcaacacg tttaccaaaa cgtttgtcct gaaacgtggt gattacgctg tcaacgtcaa
    25321 ctacaacgtg cagaacgctg gcgagaaacc gctggaaatc tccacctttg gtcagttgaa
    25381 gcaatccatc actctgccac cgcatctcga taccggaagc agcaacttcg cactgcacac
    25441 cttccgcggc gcggcgtact ccacgcctga cgagaagtac gagaaataca agttcgatac
    25501 cattgccgat aacgaaaacc tgaacatctc ttcgaaaggt ggttgggtgg caatgctgca
    25561 acagtatttc gcgacggcgt ggatcccgca taacgacggt accaacaact tctataccgc
    25621 taatctgggt aacggcatcg ccgctatcgg ctataaatct cagccggtac tggttcagcc
    25681 tggtcagact ggcgcgatga acagcaccct gtgggttggc ccggaaatcc aggacaaaat
    25741 ggcagctgtt gctccgcacc tggatctgac cgttgattac ggttggttgt ggttcatctc
    25801 tcagccgctg ttcaaactgc tgaaatggat ccatagcttt gtgggtaact ggggcttctc
    25861 cattatcatc atcaccttta tcgttcgtgg catcatgtac ccgctgacca aagcgcagta
    25921 cacctccatg gcgaagatgc gtatgctgca gccgaagatt caggcaatgc gtgagcgtct
    25981 gggcgatgac aaacagcgta tcagccagga aatgatggcg ctgtacaaag ctgagaaggt
    26041 taacccgctg ggcggctgct tcccgctgct gatccagatg ccaatcttcc tggcgttgta
    26101 ctacatgctg atgggttccg ttgaactgcg tcaggcaccg tttgcactgt ggatccacga
    26161 cctgtcggca caggacccgt actacatcct gccgatcctg atgggcgtaa cgatgttctt
    26221 cattcagaag atgtcgccga ccacagtgac cgacccgatg cagcagaaga tcatgacctt
    26281 tatgccggtc atcttcaccg tgttcttcct gtggttcccg tcaggtctgg tgctgtacta
    26341 tatcgtcagc aacctggtaa ccattattca gcagcagctg atttaccgtg gtctggaaaa
    26401 acgtggcctg catagccgcg agaagaaaaa atcctgattc ggtgagtttt cgctaaaata
    26461 agggcggtca gttgaccgcc ttttttcttt tcgtagggtg gataagcacc gcgcatccgc
    26521 cacacaaagc aacaggaaca tcatgagcga taatgacact atcgtagccc aggccacgcc
    26581 tccgggacgt ggcggcgttg gcatcctgcg catctccggc ctaaaagccc gtgaagttgc
    26641 cgaaaccgtg ctgggtaaac tgcctaagcc gcgctacgcc gattatcttc cgtttaaaga
    26701 cgccgacggc agcgtgctcg atcaggggat tgcgctatgg ttccctggcc cgaactcgtt
    26761 caccggcgaa gatgtgctgg aactgcaggg tcatggcggt ccggtgatcc tcgacctgct
    26821 gttaaaacgc attctgacca ttcccggcct gcggattgct cgccctggtg agttttccga
    26881 acgcgcgttt cttaacgata aactcgactt agcccaggcc gaggcgattg ccgatcttat
    26941 cgacgccagt tcggaacagg cggcccgttc ggcacttaac tcgctgcaag gcgcattctc
    27001 cgcacgggtc aaccatctgg tggaagccct cacccacttg cgcatttacg tcgaagcggc
    27061 aattgatttc cctgatgaag agatcgattt cctctccgac ggcaaaattg aagcccagct
    27121 taatgacgtg attgccgatc tcgatgcagt gcgtgctgaa gcacgtcagg gtagtttgtt
    27181 gcgcgaaggg atgaaggtgg tgattgccgg acgtcctaac gccggtaaat cgagcctgtt
    27241 aaacgcgctg gcgggccgtg aagcagcaat cgtaactgat atcgccggaa ccacacgtga
    27301 cgtgctgcgt gagcatatcc acattgacgg aatgccgctg catatcatcg ataccgctgg
    27361 gctacgtgaa gccagtgacg aagtggaacg tattggtatc gagcgcgcgt ggcaggaaat
    27421 tgaacaggcc gaccgcgtgc tgtttatggt cgatggcacc acaacagacg ccgtggatcc
    27481 ggcagagatc tggccggaat ttatcgcccg tctgccagcg aaactaccga tcaccgtggt
    27541 gcgcaataaa gccgatatca ccggcgaaac gctgggaatg agtgaagtga acggtcacgc
    27601 gttaattcgt ctctcggcaa ggacaggtga aggcgtggag gtgctgcgta accatctcaa
    27661 acagagcatg ggctttgaca ccaacatgga aggcggcttc ctggcgcgtc gtcgccacct
    27721 acaggcgctg gaacaggcag cggagcattt gcagcaaggt aaagcgcaac tgttaggtgc
    27781 ctgggcgggt gaactgctgg cggaagagtt gcgcctggca cagcagaact taagcgaaat
    27841 caccggggaa tttacctctg atgatttgct ggggcggatt ttctccagct tctgtattgg
    27901 taagtaaccg cgcttacgaa gccgtattct gactgtcaga tgcggcttcg cttcattgtt
    27961 agcactcctg ttattcctca accctttttt taaacattaa aattcttacg taatttataa
    28021 tctttaaaaa aagcatttaa tattgctccc
//
LOCUS       DAZPDU010000040        27108 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZPDU010000040 DAZPDU010000000
VERSION     DAZPDU010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27108
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.040"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..925
                     /locus_tag="ACHURS_001563"
     CDS             <1..925
                     /locus_tag="ACHURS_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1237330.1"
                     /translation="TSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKL
                     NDSLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPV
                     NIGANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNL
                     TAPQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQ
                     MRIDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGT
                     VNMRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(968..1828)
                     /gene="rsmI"
                     /locus_tag="ACHURS_001564"
     CDS             complement(968..1828)
                     /gene="rsmI"
                     /locus_tag="ACHURS_001564"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFR1237331.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            1892..3928
                     /gene="lpoA"
                     /locus_tag="ACHURS_001565"
     CDS             1892..3928
                     /gene="lpoA"
                     /locus_tag="ACHURS_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFR1237332.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKKQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGAML
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPVS"
     gene            3886..4281
                     /locus_tag="ACHURS_001566"
     CDS             3886..4281
                     /locus_tag="ACHURS_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFR1237333.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            4301..4891
                     /gene="diaA"
                     /locus_tag="ACHURS_001567"
     CDS             4301..4891
                     /gene="diaA"
                     /locus_tag="ACHURS_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFR1237334.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4901..5476
                     /gene="dolP"
                     /locus_tag="ACHURS_001568"
     CDS             4901..5476
                     /gene="dolP"
                     /locus_tag="ACHURS_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFR1237335.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(5590..6630)
                     /locus_tag="ACHURS_001569"
     CDS             complement(5590..6630)
                     /locus_tag="ACHURS_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR1237336.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(6703..7338)
                     /locus_tag="ACHURS_001570"
     CDS             complement(6703..7338)
                     /locus_tag="ACHURS_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFR1237337.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            7466..7984
                     /gene="yhbO"
                     /locus_tag="ACHURS_001571"
     CDS             7466..7984
                     /gene="yhbO"
                     /locus_tag="ACHURS_001571"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFR1237338.1"
                     /translation="MSKKIAVLISDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7964..8407)
                     /locus_tag="ACHURS_001572"
     CDS             complement(7964..8407)
                     /locus_tag="ACHURS_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFR1237339.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARQAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            8458..8760
                     /gene="yhbQ"
                     /locus_tag="ACHURS_001573"
     CDS             8458..8760
                     /gene="yhbQ"
                     /locus_tag="ACHURS_001573"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFR1237340.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(8747..9250)
                     /locus_tag="ACHURS_001574"
     CDS             complement(8747..9250)
                     /locus_tag="ACHURS_001574"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1237341.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(9244..9768)
                     /locus_tag="ACHURS_001575"
     CDS             complement(9244..9768)
                     /locus_tag="ACHURS_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFR1237342.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            9977..10972
                     /locus_tag="ACHURS_001576"
     CDS             9977..10972
                     /locus_tag="ACHURS_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFR1237343.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10981..11859
                     /locus_tag="ACHURS_001577"
     CDS             10981..11859
                     /locus_tag="ACHURS_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFR1237344.1"
                     /translation="MKYSLGPVLWYWPKEALEEFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDKLGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELTSMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12065..13072
                     /locus_tag="ACHURS_001578"
     CDS             12065..13072
                     /locus_tag="ACHURS_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HFR1237345.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(13190..14434)
                     /gene="mtr"
                     /locus_tag="ACHURS_001579"
     CDS             complement(13190..14434)
                     /gene="mtr"
                     /locus_tag="ACHURS_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFR1237346.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(14588..16477)
                     /gene="deaD"
                     /locus_tag="ACHURS_001580"
     CDS             complement(14588..16477)
                     /gene="deaD"
                     /locus_tag="ACHURS_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFR1237347.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(16470..16550)
                     /gene="yrbN"
                     /locus_tag="ACHURS_001581"
     CDS             complement(16470..16550)
                     /gene="yrbN"
                     /locus_tag="ACHURS_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFR1237348.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(16657..17541)
                     /gene="nlpI"
                     /locus_tag="ACHURS_001582"
     CDS             complement(16657..17541)
                     /gene="nlpI"
                     /locus_tag="ACHURS_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFR1237349.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(17650..19785)
                     /gene="pnp"
                     /locus_tag="ACHURS_001583"
     CDS             complement(17650..19785)
                     /gene="pnp"
                     /locus_tag="ACHURS_001583"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFR1237350.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20032..20301)
                     /gene="rpsO"
                     /locus_tag="ACHURS_001584"
     CDS             complement(20032..20301)
                     /gene="rpsO"
                     /locus_tag="ACHURS_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFR1237351.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(20450..21394)
                     /gene="truB"
                     /locus_tag="ACHURS_001585"
     CDS             complement(20450..21394)
                     /gene="truB"
                     /locus_tag="ACHURS_001585"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFR1237352.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(21394..21795)
                     /gene="rbfA"
                     /locus_tag="ACHURS_001586"
     CDS             complement(21394..21795)
                     /gene="rbfA"
                     /locus_tag="ACHURS_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFR1237353.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(21959..24631)
                     /gene="infB"
                     /locus_tag="ACHURS_001587"
     CDS             complement(21959..24631)
                     /gene="infB"
                     /locus_tag="ACHURS_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFR1237354.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEAHRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(24656..26143)
                     /gene="nusA"
                     /locus_tag="ACHURS_001588"
     CDS             complement(24656..26143)
                     /gene="nusA"
                     /locus_tag="ACHURS_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFR1237355.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26171..26623)
                     /gene="rimP"
                     /locus_tag="ACHURS_001589"
     CDS             complement(26171..26623)
                     /gene="rimP"
                     /locus_tag="ACHURS_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFR1237356.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(26830..26906)
                     /locus_tag="ACHURS_001590"
     tRNA            complement(26830..26906)
                     /locus_tag="ACHURS_001590"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26870..26872),aa:Met,seq:cat)
BASE COUNT         6423 a         7518 c         6809 g         6358 t
ORIGIN      
        1 tacctctgca acgctaagta gcactaactg gcaatacgcc tgttcctgct ctgcgggtaa
       61 ggcagttaaa cttgtctata tggtcagccc cgtacttacc accactggac atcagacagg
      121 atattacaaa ctcaatgaca gcctggatat taaaaccaca ttacaggcaa acgacattcc
      181 aggactcaca accgaccagg ttgtctctgt taacacccga ttcacacaga taaaaagcag
      241 caccgtatat tctgctgcaa cccaaacggg tgtttgccag ggtgacacgt ctcgttatgg
      301 acccgttaat attggtgcaa ataccacctt taccctgtat gtcaccaagc catttctcgg
      361 ctcgatgacc attccgaaaa cggatattgc cgtcattaaa ggcgcgtggg tcgatggaat
      421 gggaagcccg tctacaggtg acttccatga tttagtcaag ttatcgattc agggaaatct
      481 caccgcccca cagtcgtgca aaattaatca gggcgatgtt attaaggtta attttggatt
      541 catcaatggt cagaagttta ccacccgcaa tgccatgcca gacggtttta ctccagtaga
      601 ctttgatatc acttatgact gtggtgatac ttcaaagatt aaaaactcgt tgcaaatgcg
      661 catcgacggt acaactgggg tagtagacca gtacaacctg gtcgccaggc gaagaagttc
      721 agacaatgtg cccgatgtcg gtattcgtat tgaaaatctc ggcggcggag ttgcaaatat
      781 tccttttcag aacggtatcc ttcccgttga tccttccggg catggcaccg tcaatatgcg
      841 cgcctggcca gttaatctgg tcggtggtga gctggaaaca ggaaaatttc agggcaccgc
      901 caccattacc gtcatggtgc ggtaaaagaa gacgttgcgt tttgattaac gcaacgcttc
      961 ggcactgtta cccctgctgc tccagcgcat acttatacag cgcatttttc ttcacgccat
     1021 gaatttctgc ggccagcgcc gccgcttttt tcagcggcag ttctgcctgt agcagcgcca
     1081 gcgtgcgcag ggcatcggcg ggtaagtctt cttcctgtgc tttatgacct tcgacaatca
     1141 gcaccatttc gcctttgcga cggttttcat cttcctttac ccacgccagc agctcgccaa
     1201 cgggcgcgcc gtgaatggtt tcccaggttt tggtcagctc acgcgccaga accacgtagc
     1261 gggattcgcc taataccgca acgatatctt ccaggctatc taacagacgg tgggtagatt
     1321 cataaaaaat cagcgtgcgc ggctccgctt caatggcttt tagcgcatca cggcggcctt
     1381 ttgatttggc aggtaaaaag ccttcgtaac agaaacggtc agagggtaaa cccgcggcgc
     1441 ttaacgcagt gatagcagca cacggtccgg gtagcggcac cacacgaatc cccgcttcac
     1501 ggcaggtacg caccagatgg tagccaggat cgttaattag cggcgttccg gcatcggaaa
     1561 ccagcgcaat gttttgcccc tcttgcagct tcgccagcag cgtttcggct ttttgttgtt
     1621 cgttatggtc gtgcagcgca aacaaccggg cattaatccc aaaatgttgc agcaataaac
     1681 cggtgtgacg agtatcctcg gcggcaatca gatcaacggc ctgtaatacc tctaacgcac
     1741 gctgggtgat atccgccaga ttgccgattg gcgtcggtac aatgtaaagc tggccctgag
     1801 aattatccgc cgattggtgt tgtttcattg tgtcgtccgt attgccgatt taatattgag
     1861 cattgcgtaa aaaaatatca ctggatacat tatggtaccc tcaacatttt ctcgtttgaa
     1921 agccgcgcgt tgtctgcctg ttgttctggc agccctgatt ttcgccggtt gtggcaccca
     1981 tactcccgat cagtccactg cttatatgca gggcacggcg caggctgatt ctgcctttta
     2041 tcttcagcag atgcagcaaa gctctgatga taccaggatc aactggcaat tactcgccat
     2101 tcgtgcactg gtgaaagaag gtaaaaccgg gcaggcggtt gagttgttta accaactacc
     2161 gcaagaactg aacgattctc agcgtcgcga gaaaacactg ctggcggtag agattaaact
     2221 ggcgcagaaa gattttgctg gcgcgcaaaa cttgctggcg aaaatcacac ctgccgattt
     2281 agaacaaaac cagcaagcgc gttactggca ggcaaaaatc gatgccagcc aggggcgtcc
     2341 ttccattgat ttactgcgcg cgttaattgc tcaggaaccg ctgctcggcg cgaaagaaaa
     2401 aaagcagaat attgatgcca cctggcaggc gctctcctcc atgactcagg aacaggcgaa
     2461 tacgctggtg atcaacgccg acgaaaatat tctgcaaggc tggctggatc tgcagcgcgt
     2521 ctggtttgat aaccgtaacg atcccgacat gatgaaagcc gggatcgccg actggcaaaa
     2581 acgttatccg aacaatccgg gcgcgaaaat gctgccaacg cagttggtta acgtaaaagc
     2641 gtttaaacca gcctcgacca acaaaatcgc cctgctgttg ccgctgaatg gccaggcagc
     2701 ggtatttggt cgcaccattc agcaaggctt tgaagcggcg aaaaatatcg gcactcagcc
     2761 agtggcggct caggtagctg ccgcacctgc cgcagacgta gctgaacaac ctcagccgca
     2821 aaccgtggat ggcgttgcca gcccggcaca agcctcggtt agcgatctga ccggtgatca
     2881 gcctgcggcc cagccggtgc ctgtaagcgc cccggcgaca agcaccgcag cggtaagcgc
     2941 acccgcaaat ccatccgcag agctgaaaat ctacgatacc tcatcacaac cacttagcca
     3001 gatcttaagc caggttcagc aggatggcgc gagtattgtg gttggtccgt tgctgaaaaa
     3061 taacgttgaa gagttgctga agagcaacac cccgctgaac gtgctggcac tgaaccagcc
     3121 agagaatatc gaaaaccgcg tcaatatttg ttacttcgcg ctttcacctg aagacgaagc
     3181 gcgcgatgca gcgcgtcata ttcgtgacca gggtaaacaa gcgccgctgg tgctgatccc
     3241 acgcagttca ttgggcgatc gcgtagccaa tgcgtttgcg caagagtggc agaaactggg
     3301 cggcggcacc gttctgcaac aaaaatttgg ttccaccagc gaattacgcg cgggtgttaa
     3361 cggcggttct ggtatcgctt taacgggtac cccgattact cccagagcga caaccgactc
     3421 cggcatgacg accaacaatc caacgctgca aaccacgcca accgatgacc agttcaccaa
     3481 taatggcggt cgtgtcgatg cggtgtacat tgtggcaacg ccgggtgaaa tcgcttttat
     3541 caaaccgatg atcgccatgc gtaacggtag ccagagcggt gcaatgctgt acgccagctc
     3601 ccgcagcgca caaggcaccg caggcccgga tttccgtctg gagatggaag gtttgcagta
     3661 cagcgaaatc ccgatgctgg cgggcggtaa tctgccgtta atgcagcagg cactcagcgc
     3721 ggtgaataac gattattcac tggctcgcat gtatgcgatg ggcgtcgatg cctggtcgct
     3781 ggcaaatcat ttctcacaaa tgcgccaggt tcagggtttt gaaatcaacg gtaataccgg
     3841 aagcctgacg gcaaacccgg attgcgtgat taacaggaag ttatcatggc tacagtacca
     3901 acaaggtcag gtagtccccg tcagttaacc accaaacaga ccggcgatgc gtgggaagca
     3961 caagcgcgtc gctggctgga aggcaaagga ctgcggttta tcgccgctaa cgtgaacgag
     4021 cgtggcggcg agatcgatct gataatgcgt gaaggccgga ccaccgtttt tgtcgaggta
     4081 cgctatcgcc gctctgcgct ttatggcggc gcggcagcca gtgtgacccg cagcaaacaa
     4141 cacaaattat tacagactgc ccgcttgtgg ctcgcgcgtc ataatgggag ttttgatact
     4201 gtggattgcc ggttcgatgt ggtagccttc accgggaatg aggttgagtg gattaaggat
     4261 gcctttaatg accactcata attaaggttt aaggattagc gtgcaagaaa gaattaaagc
     4321 ttgcttcact gaaagcattc aaactcaaat tgcggcggca gaggcgcttc cggatgccat
     4381 ctcccgtgca gccatgacgc tggttcagtc tctgctcaat ggcaacaaaa tcctctgttg
     4441 tggtaatgga acttccgctg ccaatgcaca gcattttgct gccagcatga tcaaccgttt
     4501 cgaaacggag cggcccagct tacctgccat tgcactaaat actgataatg ttgtcttaac
     4561 ggcgattgcc aacgatcgct tacatgatga agtgtatgca aaacaggtgc gggcgctggg
     4621 tcatgcggga gatgtattgt tagccatttc cacccgtggc aacagccgcg atattgttaa
     4681 agcagttgaa gccgccgtta cgcgtgatat gaccattgtg gcattgaccg gctatgacgg
     4741 cggcgagctt gcaggtttgt tagggccaca ggatgtggag atccgcattc cttcgcatcg
     4801 tagtgctcgc attcaggaaa tgcatatgct gacggtaaat tgcctgtgcg atctgatcga
     4861 taacacgctt ttccctcacc aggatgatta aggagaatac atgaaggcat tatcgccaat
     4921 cgcagtcctt atttccgcgc tgctgttgca aggttgtgtt gccgctgccg tagtgggtac
     4981 cgctgctgtg ggtaccaaag ccgcaactga cccacgcagt gtcggcaccc aggtggacga
     5041 tggtaccctg gaagtgcgcg tgaacagcgc attgtcgaaa gacgaacaga ttaagaaaga
     5101 aacccgtatt aatgtcacgg cgtatcaggg caaagtgctg ctggttgggc agtcaccaaa
     5161 cgctgaactt tcggctcgcg ccaaacagat tgctatgggc gtagacggtg ccaacgaagt
     5221 gtataacgag attcgtcagg gccagccgat tggtctgggc gaagcatcta acgatacgtg
     5281 gatcaccacc aaagtgcgtt cgcagctctt aaccagcgac ctggtgaaat cgtccaacgt
     5341 gaaagtgacc accgaaaacg gtgaagtgtt cctgatgggg ctggtgactg aacgtgaagc
     5401 gaaagcggcg gcagatattg ccagccgggt gagcggcgta aagcgggtaa ccacagcgtt
     5461 tacgtttatt aaatagcagc cccttgtaat gcctgatgcg acgcttgccg cgtcttatca
     5521 ggcctacaac gatgcaaacc gtaggtcgga taaggcgttt acgccgcatc cgacatgttt
     5581 tccctcaaat cagaataaca gcgccagccc gccgacaatc acgcctgaca ctgccaccat
     5641 cgcccccgtc agccataagg ctttcgccgg gaacgctttg cgcagcataa tcagtgacgg
     5701 caaactcact gccgggagcg tcatcaacaa tgccagcgcc ggagcggttc ccatacctgc
     5761 cagcatcatc gtttgtacaa tcggaatttc tgctgccgtg ggaatgacaa acaagcatcc
     5821 tgctaccgcc atcgccacca cccacatcag gctgttatcg acagcaccat cggcatgggg
     5881 gaataaccag acgcgtgcgg cacccaacac cagtactgca aggatgtaaa ccgggatcgt
     5941 actccagaaa agtgtccata gcgccctgcc ccagcggcta aaaaaaccgc tctgtgcttc
     6001 cggtatgtca atttcgaccg gtgcctgcgt ttgcggtgtt tcacgcaccc atttttgcac
     6061 cagcgtcgca atcagcaaca ccatcaccag cccggccacc agacgaatcg ccgcaaaacc
     6121 ccagccgagg acaaacccca taaacaccag cgtcgccggg tttaacaccg gattgcccat
     6181 ccagaatgcc agcgcaccgc ccatcgatac ctgttggcga cgcattcccg ccgcgaccgg
     6241 agccgcacag caggtacaca tcatgcccgg cagcgaaaac agcgttccca gcagcgtgcc
     6301 gcgaaagcgc gattgtccaa gcgtacgcaa caaccagtca cgcgggatca gcacctgaat
     6361 caacgaaccg aggatcaccc ccagcaccgc cgctttccat accgcgagga aatagatcat
     6421 cgcgtaatcc aacgccgcct gccacggatt agcatccgcc tgcgcaagga tagatttacc
     6481 gatactgtgg gtttcggcag cagtaaacgc tttgccgtag taaggttgcc atttcacata
     6541 ccagagaccg gcaatgacaa cgagaaagaa aagcgcgggt ttccaccact gaatgggcgt
     6601 tgccgcctga gatgaagact gaccagtcat agcattcccc aggaataagt tatgataatt
     6661 gagcgcgtga atattacgct cactatcaat tcttgggaat aattattcag ctcttttgcg
     6721 taattctgaa gagcttaaaa tagtcacgcc ttcatgctca ggccgcatcg cctctgccag
     6781 catgacccgc gccacgtctc tcgcatcaat ggatttccag ttaccgggca gtaaacgaaa
     6841 cagcggcgca aaaagcgttt cgttcatccg ctgtttgcta cgatcgccca gcaacatcga
     6901 cgggcgagca atggtcaatt tcggccagtt ctgggcaatt aatgcttctt ccatctcccc
     6961 tttgacgcgg ttatagaaaa acggcgagtg ggcattggca cccatagcac tgaccaccag
     7021 catatgctgc gcgcccagtc gccgcccggt taatgcggta tccactacca gcgtgtaatc
     7081 ggcatgaata aacgcctctt tgctccccgc ttctcgccgc gtggtgccga gacaacaaaa
     7141 cacaatgtcg atgggttcgg tgacctgcgc caacgcgtca ctcagttgcg gatcatgggg
     7201 attaaacacc ccgggcatat cgcccaacgg acgtcgcgtc ggcgcggcta tggcgttaac
     7261 tttcggttcg ttaatcaaca tccgcagcag gtgaccgccc accagccccg ttgcgcctgt
     7321 aatcagtacc tgactcatct tcgctccttt acagaattgt ctgccttgcg ctccacggct
     7381 catgcaccag gcttaataga ccatgaggta attatccccg attgtgtgaa attcgcctca
     7441 tccaatgcaa caacgcggag gaagcatgag taagaaaatt gccgttttaa tcagtgatga
     7501 atttgaggat tcagaattta cttcacccgc agacgagttc cgtaaagccg gacacgaagt
     7561 gattaccatt gaaaaacaag cgggtaaaac ggtgaaaggc aaaaaaggag aagccagcgt
     7621 gaccatcgat aaatccatcg atgaagtgac gccagcggag tttgatgccc tgctgctacc
     7681 gggtggtcat tcaccggatt atctgcgtgg cgacaaccgt tttgtcacct ttacccgtga
     7741 ttttgtgaat agcggcaaac ctgttttcgc catctgtcac ggtccgcagc tgctgatcag
     7801 cgccgatgtg attcgggggc gcaaactgac cgcagttaaa ccgatcatta ttgatgttaa
     7861 aaatgcgggc gcggaatttt acgatcagga agtcgtggtt gataaagatc agctggttac
     7921 cagccggaca ccggacgatc tgccagcgtt taaccgcgag gcgttacgcc tgctcggtgc
     7981 ctgagtcgcg tagccaaatc atttttttac caaagcccag cgtgttgtca gtgaatttga
     8041 tttcatcgag ccggatttcc cacaccgggg ccgacagcat tctggcaacc ggaaagcgac
     8101 gattgtacgc ctggcgcgcg aggtcgcttt cctcaccttc cagcctgcgg atctcacctt
     8161 taaactgcac gccgcgaatt aacgctaccg ttttcggctg accgtttacc gttccggcaa
     8221 ccgcagcctg cggcccactc atctgcgcgt ggcgcgtttt ttcttccgtc aaaatgtaga
     8281 aggcgacttt ctgcgcatca aacagataaa aagcattagc gcaccacagt tccccttcct
     8341 gctgcacaca ccaggtgaca acatgttgtt ttgccaacca acggctgatg gtgatgagtg
     8401 tttccattgc tgctctcttt tatactgtgg gcctgacctt aacatgcgac gcagacgatg
     8461 acaccctggt ttctttactt gatccgtacc gccgacaata agctttatac cgggatcacc
     8521 acggatgtcg aacgccgcta tcagcagcac caaagcggca aaggggcgaa agcactgcgc
     8581 gggaaaggag aactgacgct ggcgttttct gcgccagtgg gcgatcgttc gctggcgtta
     8641 cgggcggaat atcgcgttaa gcaactgacc aaacggcaaa aagagcgtct ggtagcggaa
     8701 ggcgcagggt ttgcagagct gttaagcagt ctgcaaaccc cggagattaa aagcgattga
     8761 aatgctcgtg atactcaacc agaccggtta cgccattcag cgcgtcatcc gctaaacgat
     8821 gtacctggaa ggcgctttca gtgcccggcc agcggcaacg cagatcgtga tgcgccgcca
     8881 gttcaaagcc gaaacgactg tacagcgccg gatcgcccag cgtcaccact gcggcatagc
     8941 cgaactcatt aagcgaatcg agtccttcat agaccagttg gcgtgccagc ccctgtccac
     9001 ggtatttttc atcaaccgcc aacggtgcca tgccgaccca ttgcaggtct tcgccctgca
     9061 catcaaccgg actaaatgcc acatagccaa tgacctgacc ttcgtcatct gtcgccacca
     9121 gccccagcgt cagaaagcca tcttcacgca gatcgtgaac cagcttcgct tccgcatcac
     9181 tttcgaatga gcgacgcagc agggcatcaa tacccggcgc atcaatggga atttctactc
     9241 gaattagcat ggttcaccta ccgatgtctg tttggtttca ggcgcggttt tcattcccgc
     9301 ctcaacaaaa tccgccagtt gcagcagcat catgcgtaag gctttcggca tttgctccag
     9361 ttcaatggcg tccatcaggt ttttcacata cagccccagc tccgtatcgc cttcaatcac
     9421 cagccgacgc tggaagaaga gcgtatccgg atcttgttta cgcgccgcaa tcatcagcag
     9481 atcgctggcg tcggcactaa aactcacatc agcttgcgcg ttctggctga ccaccagttt
     9541 gccattcacc accgaggtaa accattgcag gtcaatatca cgcacatgaa tacttaacca
     9601 gcggccttca agaaactcca gctcaccatc atccagcgcc tgacggaatt gccagcttaa
     9661 gacctgctca agaacctggc gttttagcgc aaatggcgtc agttttaccg gtacactcaa
     9721 cagagatggc cccaaatgca caatacggga acgcagttta tccaacacga gttttactcc
     9781 ctgtttcaac aatcatccta ttttgccata tcagaaaaat aacatagcgg tataaatcaa
     9841 caattccata tgaaattgct gctaccacca atacaacttt aactgcctta aatcaaaaat
     9901 tgtcgcagca aggttaacta aaatcccagt tcgttaacat ttttgcgttt tgaaagcgca
     9961 accttcagga aaaattatgg agctgctctg ccctgccgga aatctcccgg cgcttaaggc
    10021 ggccatcgaa aacggcgcag atgctgttta tatcgggcta aaagatgata ctaatgcccg
    10081 tcacttcgcc ggccttaact ttaccgagaa aaaattgcag gaagcggtga gttttgtcca
    10141 tcaacatcgc cgcaaacttc acatcgcgat taacactttt gcgcatccgg acggttacgc
    10201 ccgttggcag cgcgccgtgg atatggcggc gcagctgggt gccgacgcgc tgatcctcgc
    10261 cgacctcgcc atgctggagt acgccgccga gcgttacccg catattgagc gtcatgtgtc
    10321 agtgcaggct tcggcgacca atgaagaggc gattaacttt tatcatcgcc attttgatgt
    10381 tgcccgcgtg gtgctgccgc gcgtgttgtc gattcatcag gtgaaacagc tggcacgggt
    10441 cacacctgtc ccgctggaag tctttgcttt tggcagcctg tgcattatgt cggaaggtcg
    10501 ttgctatctg tcgtcgtatc tgacgggtga gtcgcccaac accgtgggcg cgtgttctcc
    10561 ggcccgtttc gtgcgctggc aacaaacgcc gcaggggctg gaatcccgcc tgaacgaagt
    10621 gctgatcgac cgttatcagg atggcgaaaa cgcaggttat ccgacgctgt gtaaagggcg
    10681 ttatctggtg gacggcgagc gctatcacgc gctggaagaa ccaaccagtc tcaataccct
    10741 ggaactgctg ccggagttaa tggcggcgaa tattgcttcg gtgaaaattg aaggccgcca
    10801 gcgtagcccg gcgtatgtca gccaggtggc gaaagtctgg cgtcaggcta tcgaccgttg
    10861 taaggccgat ccgcaaaact tcgtaccgca aagcgcgtgg atggagacgc tcgggtcgat
    10921 gtccgaaggc acgcagacca cccttggcgc gtatcaccgt aaatggcagt gagaagagca
    10981 atgaaatatt ccttagggcc agtgctgtgg tactggccaa aagaggcgct ggaagaattt
    11041 tatcagcagg ccgccaccag cagcgccgac gtgatttatc ttggtgaagc ggtatgcagc
    11101 aagcgccggg caaccaaagt tggcgactgg ctggagatgg caaaatcgct cgccggaagc
    11161 gggaagcaga tagtgctctc cacgctggcg ctggtgcagg catcctctga actgggcgaa
    11221 ctgaaacgct atgttgagaa cggtgagttt ctgattgaag ccagcgatct cggcgtggtg
    11281 aatatgtgcg ctgaacgcaa actgccgttc gtcgccgggc acgcgctgaa ctgctacaac
    11341 gcggtgacgc tgaaaatatt gctcaaacag ggcatgatgc gctggtgtat gccggtggag
    11401 ctttcccgcg actggctggt gaatctgctt aatcagtgcg ataagttggg gattcgcaac
    11461 cagtttgaag tggaagtcct gagctacggt catctaccgc tggcctactc cgcccgctgc
    11521 tttaccgcgc gttcggaaga ccgcccgaaa gatgagtgtg aaacctgctg cattaagtat
    11581 ccgaacgggc gcaacgtgct gtcgcaggaa aaccaacaag tgtttgtact caatggcatt
    11641 cagaccatga gcggctacgt ttacaacctc ggtaacgagc tgacatccat gcagggattg
    11701 gttgatgtgg tccgcctgtc accgcagggt actgacactt tcgcgatgct cgacgccttc
    11761 cgcgctaatg aaaatggcgc tgcgccactg ccgttgacgg caaacagcga ctgtaacggc
    11821 tactggcggc ggctggctgg gctggagctg caagcctaag taaatatgct cgtcatactt
    11881 caagttgcat gtgctgcatc tgcattcgtt caccccagtc acttacttat gtaagctcct
    11941 ggggattcac tctcttgtcg ccttcctgca actcgaatta tttagagcac agctcacttt
    12001 gttaacaact ttaactactc tttaatgcag tattaaagat taatcggtaa caaagtgagc
    12061 tgttatgact gataaaacca ttgcgttttc gctactcgat ctggccccca ttcccgaagg
    12121 ttcttcagcg cgagaagcat tctcccactc tctcgatctc gcccgtctgg ctgaaaagcg
    12181 cggctatcat cgctactggc tggcagaaca ccacaatatg actggcattg ccagtgctgc
    12241 cacgtcggta ttgattggct atctggcggc gaataccacc acgctgcatc tggggtctgg
    12301 cggcgtgatg ttgcctaacc actcaccgtt ggtcattgcc gaacagttcg gcacgctcaa
    12361 tacactctat ccggggcgaa tcgatttggg gctgggtcgt gcgccaggta gtgaccaacg
    12421 gacaatgatg gcgctgcgtc gccatatgag cggcgatatt gataatttcc cccgcgatgt
    12481 cgcggagctg gtggactggt ttgacgcccg cgatcccaat ccgcatgtgc gcccggtacc
    12541 aggctatggc gagaaaatcc ccgtgtggtt gttaggctcc agcctttaca gcgcgcaact
    12601 ggcggcgcag cttggtctgc cgttcgcgtt tgcctcacac ttcgcgccgg atatgttgtt
    12661 ccaggcgctg catctttatc gcagcaactt caaaccgtcg gcacgactgg aaaaaccata
    12721 cgcgatggtg tgcatcaata ttatcgccgc cgacagcaac cgcgacgctg aatttctatt
    12781 tacctcaatg cagcaagcct ttgtgaagct gcgccgtggc gaaaccggac aactgccgcc
    12841 gccgattcaa aatatggatc agttctggtc accgtctgag cagtatggcg tgcagcaggc
    12901 gctgagtatg tcgttggtgg gtgataaagc gaaagtgcgt cacggcttgc agtcgatcct
    12961 gcgcgaaacc gacgccgatg aaattatggt taacgggcag attttcgacc accaggcgcg
    13021 cctgcattcg tttgaactgg cgatggatgt gaaggaagag ttgttgggat agtgtgtctt
    13081 aacgcgggaa gccttatccg agctggcaac gctgtcctac gtagacctga taagcgaagc
    13141 gcatcaggca ttgtgtaggc agcagaaatg tcggataagg caccgctgat tactgataca
    13201 ccggcagcaa attaaagctc gataagatat ggaccagtgc gttgccaacg ccaaacacca
    13261 gaatcagcgt aatcatcggc ttgccgcccc agacacggaa tttcgggctg ccaaagcgtt
    13321 tacgcgatgc gcgggctaac agcgccggaa caattgccgc ccagatggta gctgctaaac
    13381 cagcataacc aatggcgtac aggaatccgt tcgggaacag tagaccaccc acaacaggcg
    13441 gggcaaaggt cagcaatgcc gttttcaagc gccccagggc tgagtcatcg aaaccaaaca
    13501 gatctgccag atagtcaaac aaacccagcg ttacgccaag gaacgaactc gctaccgcaa
    13561 agtttgagaa cacgaccagc agaaggtcca ggctacggct gttcagcacg ccgcttaatg
    13621 cctgtaccag cacatcaata ttaccgccct tctctgcaat accgataaac tccggacgcg
    13681 ggatgttacc catcgtcgcc agcaaccaga tggtatacag cgccagtgcc atcagcgtac
    13741 cgtacaccag gcatttcacg atggttttcg gatctttgcc gtaatacttc atcaggctcg
    13801 gcacgttacc gtgataacca aacgatgcca gacagaacgg cagggtcatc agcaaatacg
    13861 gcgcataaga tgcattgctt tcggcgacgt tgaacaatgt cgcaggctgc acatgcccca
    13921 gtaggctacc gaaggtgagg aagaaggtaa tgactttcgc ccccagtaca atcgccgtca
    13981 tgcggctgac ggccttagta ctcaaccaca ccacaaacgc taccagcagt gcaaaaccaa
    14041 aacccgccgc ccgtgccggg acgtttagcg acatctctgc gaaggtgtga tgcagaattg
    14101 aaccactggc agaaatatag gcataggtca ggatatagag cacaaaggca atggaaatac
    14161 cgttgaccac gttccagcct ttgcccagca aatctttggt gatggtgtca aaactcgaac
    14221 cgattcggta attcaggtta gcttccagaa tcatcaagcc ggaatgcagc atacagaacc
    14281 aggtaaagat cagcgccgcc attgaccaga aaaaccacgc cccggacatg accactggca
    14341 gagaaaacat ccctgcgcca ataatggtgc cgccgataat caccacgccg ccaagcagcg
    14401 acggtgacgt ttgggtggtg gttagtgttg ccatgagagc ttctctccag tgaaaaatag
    14461 tgcgactgcg ttgttatgca ttgcactgta ccagtacacg agtacaaaag acagaaaaaa
    14521 agccccgatg gtaaaaatcg gggctgtata tattatttta cagattgtgt tcgctgttca
    14581 gcgatgatta cgcatcacca ccgaaacgac gacgaccggt agagtcatca cgacgcggag
    14641 cgcggccttc acgacgttcg ccgctaaaac gacgaccatc accacggcca ccttcacggc
    14701 gttcaccgct gaagttacga ccgccttcac gacgttcgcc accgaaacca cgaccaccgc
    14761 cacgacgctc accgccagta tgcggctgtg catcgcccag taactgcatg ttcatcggct
    14821 tgttgagaat gcgagtgcgc gtaaagtgtt gcagcacttc acccggcata cctttcggca
    14881 gttcgatggt ggagtgagaa gcaaacagct tgatgttacc aatgtaacgg ctactgatgt
    14941 cgccttcgtt agcaatcgca ccaacgatat gacgaacttc aacaccatca tcgcggccca
    15001 cttcaatgcg gtacagctgc atatcgccaa catcacgacg ttcacgacgc ggacgatctt
    15061 cacggtcacc acgcgggcca cggtcgttac gatcgcgcgg accacggtca tcacggtcac
    15121 ggaattcacg tttcggacgc atcggcgcat ctggcggtac gatcagagta cgttcaccct
    15181 gtgccatttt cagcagtgcc gcagccagag tttcgagatc cagctcttca ccttctgcag
    15241 tcggctgaat tttgctcagc agtgcgcggt attgatccag atcgctgctt tccagctgct
    15301 gctgtacttt agcggcgaat ttttccagac ggcgtttgcc cagcagttct gcgttcggca
    15361 gttctacttc cggaatagtc agcttcatag tacgttcaat gttgcgcagc agacgacgct
    15421 cgcggttctc aacgaacagc agcgcgcggc cagcacgacc cgcacgaccg gtacgaccga
    15481 tacggtgaac gtaagactca gaatccatcg ggatatcgta gttaactacc aggctgatac
    15541 gctcaacgtc caggccacgg gctgcaacgt cggtcgcaat caggatgtcc agacgaccat
    15601 ctttcaggcg ttccagtgtc tgttcacgca gcgcctggtt catgtcaccg ttcagcgcgg
    15661 cgctgttgta gccgttacgc tcaagagctt cagccacttc cagagtcgcg tttttggtac
    15721 gaacgaagat aatcgccgca tcaaaatctt ccgcttccag gaaacgtacc agtgcttcgt
    15781 ttttgcgcat accccagaca gtccagtagc tctggctgat gtcaggacgg gtagtcacgc
    15841 tggactgaat gcgcacttcc tgcggctctt tcataaagcg gcgggtaatg cgacgaatcg
    15901 cttccggcat ggttgcagag aacagagcgg tctgatgacc ttccgggatc tgcgccataa
    15961 tggtttcaac gtcttcgatg aagcccatgc gcagcatttc gtcagcttca tccagaacca
    16021 gaccgctcag tttagagagg tccagagtgc cacgtttcag gtggtccagc agacggcccg
    16081 gagtaccgac aacgatctgc ggcccctgac gcagggcgcg taattgcacg tcataacgct
    16141 ggccgccgta cagagcaacc acatttacgc cgcgcatgtg tttagagaaa tccgtcattg
    16201 cttcagcaac ctgtaccgcc agttcgcggg tcggtgccag caccagaatc tgtggtgctt
    16261 tcagctcagg atcaagattc tgcaacagag gtaaagagaa tgctgcagtt tttccgctcc
    16321 ccgtctgggc catacccaga acgtcgcggc cattcagcag atgtggaata cactctgcct
    16381 gaattggaga tggtttttcg taacccagat cgttaagggc ttcaaggata ggagccttca
    16441 ggcccagatc tgcaaaagtg gtttcgaatt cagccatgta gtacgtgtgc ctcaaaatta
    16501 atggcggcca gtctacataa ctcatcatga aattgatcag caattttcat tgaaaagtgt
    16561 gaaccggctc aaagtaggtg tattaacgaa caacaacgcc ctcacccgtt aaggtgatgg
    16621 caatcaaaaa agattacggg ctgatgtgta cgtcagctat tgctggtccg attctgccag
    16681 gtcatcttgg tcctggccca ggagcgataa ttccaacaat gcgtatcggt gctcaacaaa
    16741 gttatgaacg ttgttggcaa ccgccagttt gaacagtgcc gtggcgctgt ccaaatcccc
    16801 cagacttagg tagtacttac ctaaatagaa gttggtttca ctgagatgct cagcgagcga
    16861 ggtgttatcc gttgcgtccg ccttgagcct ttccattaac gtttgttcgc taatgttgcc
    16921 caggtagaac tcgacaatgt tccatcccca ctgttcctta tccgattttt cgaagtgctg
    16981 tttcaacact tctttagcct gcttctcatc gagcttctgc tcggcgagat aaagccacag
    17041 actacggaaa ggatcattgg gatcgtcttg ataaaacgcc agcagatcat cttgcgctaa
    17101 cttgtcacga ccgccgtaat ataatgcgat cccgcgattc aagtgcgcgt agttgtaagt
    17161 tggatcaagc tcaagtacag aatcaaacgc ttcataggca gcatcaaaat tgcctgcctg
    17221 cgttaaatat atgcctaagt aattgaatac ttcaggcata tccggtcgga ttgccagcgc
    17281 ttgcgaaaaa tcgttacgcg ctaatgccct cagaccgaga ctatcataca acactccgcg
    17341 ctcatataaa agctgtgcgc gttcgtcatc ggttaaagcc cgactggcaa ggatttgttc
    17401 catacgtgcc agaatcactt cctgctgtaa agtcggttgc aatggtaccg cgaggacttc
    17461 acttttacgc caggaagtat tactgcatcc tgcaagcgta agtgctgtcg caacgaaaca
    17521 ccagcgcaaa aaaggcttca tttcccactc ccgaagacca cggttgaatg aacgtcctgt
    17581 tcccggttgc taacaaggcg tcctgcccgg ttaaaagccc cccgccgcag cggagggcaa
    17641 atggcaacct tactcgccct gttcagcagc cggagcttcc ggtgctgcag caggttgaga
    17701 ctgctcagtc gcttctttaa tgctcagacg gatacggccc tggcgatcaa cttctagaac
    17761 tttcaccggt acttcctgac ccatctgcag gtaatcggtc actttctcaa cgcgtttgtc
    17821 agcgatttga gagatgtgga ccagaccttc tttaccgccg ccgatggcaa caaatgcgcc
    17881 aaagtcaacg atacgggtca ctttaccagt gtagacgcgg cccacttcga tttctgcagt
    17941 gatctcttcg atacgacgaa tagcatgttt cgctttctcg ccgtcggtcg ctgcgatctt
    18001 cacagtaccg tcatcttcga tttcgatggt agtgccagtt tcttcggtca gggcacggat
    18061 aacagagccg cctttaccga taacgtcttt gatcttatcc gggttgatct tgatggtatg
    18121 gatacgcggt gcgaactcag agatatcgcc acgcggcgcg ttgatcgcct gttccattac
    18181 gcccaggata tgcagacgcg cacctttagc ctggttcagc gcaacctgca tgatctcttt
    18241 ggtgatacct tcaattttga tatccatctg cagtgcagag ataccgtcgc gggaacctgc
    18301 aactttgaag tccatatcgc ccaggtgatc ttcgtcgccc aaaatgtcag acagtacaac
    18361 gtagttgtcg ccttctttca ccagacccat tgcgataccc gcaacggcag ctttgattgg
    18421 cacacctgcg tccatcagcg ccagagacgc gccgcacacg gaagccatag aagaagaacc
    18481 gttggattcg gtgatttcag acactacacg tacggtgtac gggaatttgt ccatatccgg
    18541 catgactgcc agcacgccgc gcttcgccag acgaccgtga ccaatttcac gacgcttcgg
    18601 agaaccgacc atgccggttt cgcctacgga gtacggaggg aagttgtagt ggaacaggaa
    18661 ggtatcggta cgttcgccca tcagttcatc aagaacctgc gcgtcacgag cggtacccag
    18721 cgttgcagta accagtgcct gcgtttcacc gcgggtgaac agcgcagaac cgtgagtacg
    18781 cggcagcacg ccagtacgca catccagacc acggatcata tctttttcac gaccgtcgat
    18841 acgcggttcg cctgccagta cgcggctacg aacaacgttt ttctcgatag cgtgcagaat
    18901 ttcacccagt tcgttttcgt ccagggtttc gtcttcagca agcagcgtcg cgatggtttc
    18961 agatttgatg acatcaacct gcgcataacg ctcttgtttg tcggtgatgc ggtaagcatc
    19021 actcagacga gcttcagcca gtgcagcaac gcgcgcgttc agcgcttcgt ttaccggctc
    19081 cggctgccag tcccaacgcg gtttaccggc ttctttcact agttcattga tgttctgaat
    19141 aacaacctgc tgttgttcat gaccgaacac tactgcgccc agcatctgat cttcgctcag
    19201 cagttcagct tcagattcaa ccatcagtac agcggcttca gtaccggcaa caaccagatc
    19261 cagtttgctc tctttcagct cgtcctgagt cgggttcagt acgtactggt cattgatgta
    19321 acctacgcgg gcagcaccaa tcgggccatt gaacggaata ccagacagag acagtgctgc
    19381 ggaagcacca atcatcgcga cgatatccgg gttaacctgc gggttaacag aaaccacggt
    19441 ggcgataacc tgaacttcgt tgacgaagcc ttccgggaac agcgggcgaa tcgggcggtc
    19501 aatcagacgc gcgatcaggg tttcgccttc gcttggacga ccttcacgac ggaagaagct
    19561 acccgggata cgaccagcag cgtaggtacg ctcctgatag ttaacggtca gtgggaagaa
    19621 gtcctgacct ggtttggctt ttttctgtcc aacaacggta acgaataccg cggtgtcatc
    19681 catgctaacc ataacagcgg cagtagcctg acgagccatc atgccggttt ccagagtcac
    19741 ggtgtgttgg ccgtactgga atttacgaac gatcggatta agcaatgtaa tatcctttct
    19801 ctttcttaga cagtacctta cggcactggt gttaataccc gatcttctgc gcatcctcgc
    19861 gactaatgac aaccctaacc cagctctatg tgggtaaagc ctctcattag ccgcgcgaac
    19921 ctctgcaacg gaagatcatt catagcaaca atacattagt ttccagtgaa ttgctgccgt
    19981 cagcttgaaa aaaggggcca ctcaggcccc cttttctgaa actcgcaaga attagcgacg
    20041 cagacccagg cgctcgatga gctgggtgta acgtgctacg tctttacgtt tcaggtagtc
    20101 gagcagttta cgacgctgag aaaccatgcg cagcagacca cgacggctgt ggtgatcttt
    20161 tttgtgctct gcaaagtggc cctgcaggtg gttgatctgt gcagtcagca gtgctacctg
    20221 aacttcggta gaaccggtgt cgtttgcgtc acgaccaaac tcagaaacga ttttagctgt
    20281 tgcttcagta cttagagaca ttttaaaact ccaaagtata tagaatgaaa ggacgccgat
    20341 ctctaattca gcgatcccag tgtacgttac gcaaagtgtt aaacaattta cgcgacgtta
    20401 agcggcagta ttctactcgt agcgacctgt tatcgcaaga cggttaacat tacgccgggt
    20461 attcaaccac caggcgacga ggcgcgacgc ggccttcatc gtcaatttcg cccataccga
    20521 taaacttgcc gttctcacct tctgtaacgc gaaccagtcc ttccagcggc gcaccggatg
    20581 tacgaactgg gttaccattt ttgaagtaaa cagaagacgt taacggaaga ttcaccaccg
    20641 ggtagtccga agctggactg tccattggca tcagtaatgg atcaagtaac tccgcagccg
    20701 gaatatcctg ctgttcggct tgttcgacca gttcacgcag atgttccagg gtcaccatcc
    20761 gttcaaccgg atatttactt accgccagac gacgcaggta aataacatgc gcgccacaac
    20821 cgagtttttc acccaggtca tcaatgatgg tgcggatata agtgcctttt gagcagtgaa
    20881 tttccagctc cagctcattg ccttcgtggc gaataaacag caattcataa acggtaatcg
    20941 ggcgcgcttc acgcggtact tcaatgccct gacgcgcata ttcgtacagt tttttaccct
    21001 gatatttcag tgctgaatac atcgaaggga tctgttcgat atcgccacgg aaagtatcca
    21061 gcgccgccgc cagttgctct gcgctaaagg ttaccggacg ttcttcaacg atttgtccgt
    21121 cggcatcaga agtatcggta cgctgtccaa gacgcgcaat gacccgatag cgtttgtcgg
    21181 agtccagcag atactgggaa aacttcgtcg cttccccgag gcaaattggc aacatgcctg
    21241 ttgccagcgg gtccagcgca ccggtatgcc cggcacggtt ggcgttataa atccgtttca
    21301 ctttttgcag cgcatcattg ctggacatac cctgcggttt atccagcaac aaaacgccgt
    21361 taatgtcgcg accgcgacga cgaggacgac tcattagtcc tccttgctgt cgtccgggtt
    21421 aacacgacgt tcttcgtcat gtttgaccac gctggtcacc aggtttgaca tgcgcatccc
    21481 ttcaaccaga gagttgtcgt agaagaaggt cagttccggc acgatacgca ggcgcatcgc
    21541 tttccccagc aggctgcgga tgaaaccaga agcttcttgc aacgctttga tgcccgcttt
    21601 aaccgcgtct tcatctttgt cgttgaggaa cgtcacatat actttggcat acgccaggtc
    21661 gcgagacatt tcgacaccgg aaacggtggt catcatgccc aggcgaggat ctttaatttc
    21721 acgctgcagg atgagagcga tctctttttg catttcctgc gctacgcgct gcgggcgacc
    21781 aaattctttc gccataataa attctcctga caaaaaaggg gctgttagcc cctttttaaa
    21841 attaatttca ggtggaaggg ctgttcacgt tggcctgata agacgcgcca gcgtcacatc
    21901 aggcaatcca tgccggatgc ggcgtaaacg ccttatcccg catggaaccc taaaaacctt
    21961 aagcgatggt acgttggatc tcaatgattt cgaatacttc gatcacatcg ccagtgcgga
    22021 cgtcgttgta gttcttaacg ccgataccac attccatacc gttacggact tcgttaacgt
    22081 catctttgaa gcggcgcagg gactccagct cgccttcgta gataaccacg ttgtcacgca
    22141 gaacgcggat cgggttgtga cgtttaacca caccttcggt aaccatacag cctgcgatgg
    22201 caccaaattt cggcgatttg aacacgtcac gaacttccgc cagaccgata atctgctgtt
    22261 tcagttccgg agacagcata ccgctcatcg ccgctttcac ttcgtcaatc aggttataga
    22321 tgacggagta gtaacgcaga tccaagcttt ccgcttcaat cactttacgt gcagaggcat
    22381 cagcacgtac gttgaagcca accaggatgg cgttggacgc cgcagccagg gtggcgtcgg
    22441 tttcggtgat accacctacg ccagaaccga tgatcttcac tttaacttcg tcagtagaca
    22501 gtttcagcaa ggagtcggag atcgcttcca cagaaccctg tacgtctgcc ttcaggacga
    22561 tgttcacttc gtgaacttcg ccttcggtca tgttggcgaa catgttctcg agtttagatt
    22621 tctgctgacg cgccagttta acttcgcgga atttaccctg acgatacagt gcaacttcac
    22681 gcgctttctt ctcgtcacgt acaacggtaa cttcatcacc cgcagccggt acgccggaca
    22741 ggccgaggat ttccaccgga atggacggac ccgcttccag cacttcctga cccagttcgt
    22801 tacgcatcgc acgaacacga ccgtattcga agccacacag aacgatatcg cccttgtgca
    22861 gagtaccttc acgtaccaga acggtagcaa ccggaccacg acctttatcg aggaaggatt
    22921 cgataaccgc accgctcgcc atacctttac gtaccgcttt cagctccaga acttccgcct
    22981 gcagcaggat agcgtccagc agttcatcga tgccggtacc cgctttcgca gatacgtgta
    23041 cgaactggct ttcaccgccc cactcttccg gcaggatgcc atactgggag agttcgttct
    23101 taacacgatc cggatcggct tctggtttat caatcttgtt cactgcaacc accaccggta
    23161 cctgcgccgc tttcgcgtgc tggattgctt cgatggtctg cggcatcacg ccgtcgtcgg
    23221 cagcaacaac caggactacg atgtccgttg cctgcgcacc acgtgcacgc attgaagtaa
    23281 acgcggcgtg ccccggggtg tccaggaagg tgatcatgcc gttttcagtt tcaacgtggt
    23341 atgcaccaat gtgctgggta atgccgcccg cttcgccaga ggcaactttc gttgaacgaa
    23401 tgtagtccag cagagaggtt ttaccgtggt caacgtgacc catgatggtc acaaccggcg
    23461 cgcgcggttc agccgcagca cccgtgtcac ggtcgctcat taccgcctct tccagctcgt
    23521 tttcacgacg caggataact ttgtggccca tctcttcagc aaccagctgt gcggtttcct
    23581 gatcgataac ctggttgatg gttgccattg cgcccagttt catcatcgct ttgatgacct
    23641 gagagccttt aaccgccatc ttgttcgcca gttcgccaac ggtgatggtt tcgccgatca
    23701 caacgtcacg gttaacggcc tgagctggct tctggaagcc ttgctgcagg gaagaacctt
    23761 tacgttttcc gcctttaccg ccacgtactg ctgcgcgtgc ttcttcacga tcagcttttg
    23821 attcagcgtg tttgttgcct ttcttcggac gcgctgcttt cgcgttacga ccacggccac
    23881 gaccgccttc gacttcacga tcgctttcgt cttctgcctg gcgagcatgt tgagaagtag
    23941 tgacgtgata atcgctggaa tcttcagtcg gttccgcgtt atcagtccat ttgttttctt
    24001 ccgccatacg acgtgcttct tcagcaacgc gacgtgcttc ttcttcgagt ttacgatgcg
    24061 cttcttcttc ggctttacgc ttgagctctg cagcttcctg ctcacggcgg gctttttcag
    24121 cctgggcgtt tttagtcata tcgtcttgtt gattgctcac tttgtctttt tccgcagctt
    24181 cacgtttcgc ttgttcagca gcttcacgct tagcttgttc tgcagcctca cgttcagctt
    24241 tttgttgcgc ctcgcgttta gccgattctt ctgcctcacg acgggcttgc tcttccgctt
    24301 cacgctgcgc ttgctcttcc gctgcaaggc gttcagcctc ttgcggatcg cgtttcacaa
    24361 aggtgcgttt cttgcggact tcgatttgta ccgatttgct ttttccaccg gtaccaggaa
    24421 tgttaagggt gctgcgtgtt ttacgttgca gcgtcaattt gtccgggcct gaatttttct
    24481 gattcaggtg gtcaatcaaa gtctgtttct cttgtgcaga cacagagtcg tcagcagact
    24541 tccggatacc tgcatcagca aattgctgta ccaggcgttc cacggaggtc tgtcgctctg
    24601 cggccagcgt tttaatcgtt acatctgtca tgctgttcct tcctgctaca gtttattacg
    24661 cttcgtcacc gaaccagcaa atattacggg cagccataat cagtgctccg gccttttcgt
    24721 cggtcaaccc ttcgatatca gccagatcat caatgccctg ttcggcgaga tcttccagcg
    24781 tacaaacgcc acgggcggcc agtttgaatg ccaaatcacg atctacccct tcaaggttca
    24841 gcagatcgtc agccggtttg ttatcaccga ggctttcttc ctgggcctgt gcaatggtgg
    24901 ccagtgcatt tttagcacgc tcgcgcagtg cttcaacggt cggctcatca aggccttcga
    24961 tttccaacag ctctttcatc ggcacatagg ccagttcttc cagcgtcgag aagccttctt
    25021 ctaccagaac agtcgcgaag tcttcgtcga tgtcgagata tttggtgaag gtgtcgatcg
    25081 ctgcgtgcgc ttccgcctga tgcttagcct gcaggtcgtc aacggtcatc acgttgagtt
    25141 cccaaccgct cagctgcgaa gccagacgca cgttctgacc gttacggcca atcgcctgcg
    25201 ccaggttacc ggcttcaacg gcgatatcca tggtgtgttt atcttcatcc accacgatag
    25261 aagcaacgtc tgccggtgcc attgcgttaa tcacgaactg cgccgggtta tcatcccaca
    25321 ggacgatatc gatacgctcg ccacccagtt cagtagacac cgcctgaaca cgcgcgccac
    25381 gcatacctac gcaagcacct accggatcga tacgtttatc gttggttttc accgcgattt
    25441 tcgcacgaga acccggatcg cgagccgctg ctttaatttc aatcacttct tcgccgattt
    25501 ctggcacttc aatacggaac agttcgatca gcatttccgg cttggaacga gtgacgaaca
    25561 gttgcgcgcc acgcgcttcc gggcgaacgg aataaagcac gccacgaacg cggtcgccag
    25621 ggcggaagtt ttcacgcggc agcatatctt cacgcaggat cacggcttca gcgttgttgc
    25681 ccagatccag agagatgttg tcgcggttta cttttttcac cacgccggtg atgatttcac
    25741 cttcgtgttc acggaactga tcaaccacca tcgcacgttc ggcttcacgc actttctgca
    25801 cgataacctg ttttgccgtc tgggtagtga tacggtcaaa ggtaacagac tcaatctgat
    25861 cttcaacgta atcgcccagg ttcaggcttt catcttcata acgtgcggct tcaagggtga
    25921 tttccttggt cggctgggtg acttcatcaa caactaacca gcgacggaag gtgtcaaaat
    25981 caccgctttt gcgatcgatc tgtacgcgga cgtcgatctc ttgttcatat tttttctttg
    26041 ttgctgtcgc cagcgcgctt tccaatgctt cgaaaatctt ctcgcgaggt agcgcctttt
    26101 cattggatac ggcttcaact acagccaaaa tttctttgtt catcgcgggc ttttcacctc
    26161 atccagacta ttaaaagtgg ggaaccaggt tcgccttctg gatattactc agcgcgaaca
    26221 cttcatcttt accttcgacg gtaactgtga tcatttcacc gtctaccgct ttgataacgc
    26281 cctgccattt acgacggttt tgtaccgcca tacggagaac cagagtcacc tcttctccga
    26341 caaaacgggc gtagtgttca gccgtgaaca gtgggcgatc gagacccggt gaggagactt
    26401 ccaggttata agcaacggtg atgggatctt caacatccag cacagcactt acctggtggc
    26461 tcacatcagc acaatcatca acattgatgc catcttcact atcaatatag atgcgcagtg
    26521 tggatgtgcg accgcgaata aattcgatgc caaccagttc aaaacccagg gcctcaactg
    26581 gcgcagtaat catctctgtt aatttttgct ctaatgtgga caagcccacc cccaagacat
    26641 aaaaaaaggg cctaaagccc agttattctg tagtcagata acaaaaaacc ccgataaatc
    26701 ggggctttat ataactgaac cctataaccg caactgcggt ctggagcact ttccagaagg
    26761 attttttcaa atcccactac gaaggccgaa gtcttcacag tatatttgaa aaaggactct
    26821 aagggaaagt ggttgcgggg gccggatttg aaccgacgac cttcgggtta tgagcccgac
    26881 gagctaccag gctgctccac cccgcgcctg aaacgtggca aattctactc gttttgggta
    26941 aaaaatgcaa atactgctgg gatttggtgt accgagacgg gacgtaaaat ctgcaggcat
    27001 tatagtgatc cacgccacat tttgtcaacg tttattgcta atcatgtgaa tgaatatcca
    27061 gttcactttc atttgttgaa tacttttgcc ttctcctgct ccccctta
//
LOCUS       DAZPDU010000041        26514 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZPDU010000041 DAZPDU010000000
VERSION     DAZPDU010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26514
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.041"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            11..334
                     /locus_tag="ACHURS_001591"
     CDS             11..334
                     /locus_tag="ACHURS_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFR1237357.1"
                     /translation="MKWVVIDTVIQPTCGISFSAIWGDMKMIIWYQSTIFLPPGSIFT
                     PVKLGIILKEKEYPITIYNIAPFNKNLWSLLKSSQECPPGESKITNKCLHNSCIIKIC
                     PYGLK"
     gene            complement(437..1687)
                     /gene="icd"
                     /locus_tag="ACHURS_001592"
     CDS             complement(437..1687)
                     /gene="icd"
                     /locus_tag="ACHURS_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFR1237358.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGEAIIENM"
     gene            1859..2512
                     /gene="rluE"
                     /locus_tag="ACHURS_001593"
     CDS             1859..2512
                     /gene="rluE"
                     /locus_tag="ACHURS_001593"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFR1237359.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYTLDNLANGEWREATE"
     gene            2522..2983
                     /gene="nudJ"
                     /locus_tag="ACHURS_001594"
     CDS             2522..2983
                     /gene="nudJ"
                     /locus_tag="ACHURS_001594"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFR1237360.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            3037..4143
                     /gene="mnmA"
                     /locus_tag="ACHURS_001595"
                     /gene_synonym="trmU"
     CDS             3037..4143
                     /gene="mnmA"
                     /locus_tag="ACHURS_001595"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFR1237361.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPGQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            4179..4820
                     /gene="hflD"
                     /locus_tag="ACHURS_001596"
     CDS             4179..4820
                     /gene="hflD"
                     /locus_tag="ACHURS_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFR1237362.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4824..6194
                     /gene="purB"
                     /locus_tag="ACHURS_001597"
     CDS             4824..6194
                     /gene="purB"
                     /locus_tag="ACHURS_001597"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFR1237363.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6362..7033
                     /gene="phoP"
                     /locus_tag="ACHURS_001598"
     CDS             6362..7033
                     /gene="phoP"
                     /locus_tag="ACHURS_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFR1237364.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            7033..8493
                     /gene="phoQ"
                     /locus_tag="ACHURS_001599"
     CDS             7033..8493
                     /gene="phoQ"
                     /locus_tag="ACHURS_001599"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFR1237365.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            8569..9690
                     /gene="roxA"
                     /locus_tag="ACHURS_001600"
                     /gene_synonym="ycfD"
     CDS             8569..9690
                     /gene="roxA"
                     /locus_tag="ACHURS_001600"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFR1237366.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9739..10965)
                     /gene="pepT"
                     /locus_tag="ACHURS_001601"
     CDS             complement(9739..10965)
                     /gene="pepT"
                     /locus_tag="ACHURS_001601"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFR1237367.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            11215..12351
                     /gene="potA"
                     /locus_tag="ACHURS_001602"
     CDS             11215..12351
                     /gene="potA"
                     /locus_tag="ACHURS_001602"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFR1237368.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            12335..13192
                     /gene="potB"
                     /locus_tag="ACHURS_001603"
     CDS             12335..13192
                     /gene="potB"
                     /locus_tag="ACHURS_001603"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFR1237369.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            13189..13983
                     /gene="potC"
                     /locus_tag="ACHURS_001604"
     CDS             13189..13983
                     /gene="potC"
                     /locus_tag="ACHURS_001604"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFR1237370.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            13980..15026
                     /gene="potD"
                     /locus_tag="ACHURS_001605"
     CDS             13980..15026
                     /gene="potD"
                     /locus_tag="ACHURS_001605"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFR1237371.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKAIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(15182..16003)
                     /gene="cobB"
                     /locus_tag="ACHURS_001606"
     CDS             complement(15182..16003)
                     /gene="cobB"
                     /locus_tag="ACHURS_001606"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFR1237372.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(16019..16930)
                     /gene="nagK"
                     /locus_tag="ACHURS_001607"
     CDS             complement(16019..16930)
                     /gene="nagK"
                     /locus_tag="ACHURS_001607"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFR1237373.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQIGCIENYLSGRGFAWLWQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDSDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(16959..18203)
                     /gene="lolE"
                     /locus_tag="ACHURS_001608"
     CDS             complement(16959..18203)
                     /gene="lolE"
                     /locus_tag="ACHURS_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFR1237374.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGTNLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(18203..18904)
                     /gene="lolD"
                     /locus_tag="ACHURS_001609"
     CDS             complement(18203..18904)
                     /gene="lolD"
                     /locus_tag="ACHURS_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFR1237375.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(18897..20096)
                     /gene="lolC"
                     /locus_tag="ACHURS_001610"
     CDS             complement(18897..20096)
                     /gene="lolC"
                     /locus_tag="ACHURS_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFR1237376.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            20358..21431
                     /locus_tag="ACHURS_001611"
     CDS             20358..21431
                     /locus_tag="ACHURS_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFR1237377.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            21559..25005
                     /gene="mfd"
                     /locus_tag="ACHURS_001612"
     CDS             21559..25005
                     /gene="mfd"
                     /locus_tag="ACHURS_001612"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFR1237378.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            25149..26105
                     /gene="ldtC"
                     /locus_tag="ACHURS_001613"
                     /pseudo
     CDS             25149..26105
                     /gene="ldtC"
                     /locus_tag="ACHURS_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
     gene            complement(26188..26445)
                     /gene="bhsA"
                     /locus_tag="ACHURS_001614"
                     /gene_synonym="ycfR"
     CDS             complement(26188..26445)
                     /gene="bhsA"
                     /locus_tag="ACHURS_001614"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFR1237379.1"
                     /translation="MKNVKTLIAAAILSSMSFANFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         6473 a         6876 c         6718 g         6447 t
ORIGIN      
        1 gacataacaa atgaagtggg tagtaattga cacggtaatt caacctacat gcggcatatc
       61 tttttcagcc atatggggtg atatgaaaat gatcatctgg tatcaatcta ctatatttct
      121 tcctcctgga agtatattta caccggttaa gcttggtatt atccttaagg aaaaagaata
      181 tcctattact atttataaca ttgcaccatt caacaagaat ttatggagtt tactcaaaag
      241 cagtcaagag tgtcctccag gagaaagcaa aataacaaat aaatgtttac ataatagttg
      301 cattataaaa atatgcccat atgggctcaa gtaatgggct tcatataaat atgctcgata
      361 tgctattaat aatatccaca taaataaaac aacgggcgtg ttatacgccc gtttcaatat
      421 ttaactcatg gagagattac atgttttcga tgatcgcttc accaaactct gaacatttca
      481 gcagcttagc gccttccatc agacgttcaa agtcataagt tacggttttc gcgttgatag
      541 caccttccat acctttaaca attaaatcag ccgcttcagt ccaccccata tggcgcagca
      601 tcatctcagc ggagaggatg atagagcctg gatttacttt gtcctgaccg gcatatttcg
      661 gcgcagtacc gtgggtggct tcaaacaggg cgcattcgtc accgatgttt gcgccagggg
      721 cgataccgat accgccaacc tgcgctgcca gggcgtcaga aatgtagtca ccgttcaggt
      781 tcatacaggc gataacatca tattcagccg gacgcagcag gatctgttgc aggaatgcat
      841 cagcaatcac gtctttaatg acgatctctt tgccggtgtt cgggttttta actttcagcc
      901 acgggccgcc gtcgatcagt tcaccgccaa actcttcacg cgccagctgg tagccccagt
      961 ctttaaacgc gccttcggtg aacttcatga tgttgccttt gtgcaccaga gtcacagagt
     1021 cacgatcgtt agcaattgcg tattcgatcg ctgcacgaac cagacgtttg gtgccttctt
     1081 ccgaacacgg cttaataccg ataccacaat gttccgggaa gcgaattttc ttcaccccca
     1141 tctcttcacg caggaattta atcactttct cggcgtcggc agagtctgct ttccattcga
     1201 tacccgcata aatgtcttcc gagttttcac ggaagataac catatcggtc agttcagggt
     1261 gtttaaccgg gcttggagtg ccctgatagt aacgtaccgg acgcaggcag atgtagagat
     1321 ccagttcctg gcgcagggca acgttcagag agcgaatacc gccaccaaca ggagtggtca
     1381 gcggaccttt aatggcaacg cgatattcac gaatcagatc aagggtttca gcaggcagcc
     1441 agacatcctg accataaacc tgtgtggatt tttcaccggt gtaaatttcc atccaggaga
     1501 ttttacgctc gcctttatag gctttctcga ctgcagcgtc gaccactttc agcatggctg
     1561 gggttacatc tacaccgatt ccatcacctt caatgtaagg gataatcgga ttttcaggaa
     1621 cgttgagttt gccgttttgc agggtgatct tcttgccttg tgccggaaca actactttac
     1681 tttccattca cctctccttc gagcgctact ggtttgctcg tctgccacca cgttgcatat
     1741 gcgtttgcgt cctgcgatac ggatgcttta gagcaatttt ttgttaatga tttgtaattg
     1801 gcttgtcaat gctaccctat tgtttgccgt catgaaagat gcgcgttatt aggctataat
     1861 gcggcaattc ataatctctg aaaataccat gcaaaaaact tcttttagaa atcaccaggt
     1921 taagcgattc agctcgcaac gttctactag gcgtaaacct gaaaaccagc ccacgcgtgt
     1981 gatcctgttc aataaaccct acgatgttct tccgcagttc accgatgaag ccggacgcaa
     2041 aacattaaaa gaattcatcc cggttcaggg cgtttatgca gcaggtcgtc ttgaccgcga
     2101 tagcgaaggg ttactggtgc tgaccaataa cggcgcgttg caggcgcgtt taacccagcc
     2161 gggcaaacgc accgggaaaa tctattatgt gcaggtggaa ggtattccca cacaagacgc
     2221 acttgaagcc ttgcgcaatg gcgtaacctt aaatgatggc cctaccctgc ccgccggcgc
     2281 ggagctggtt gacgaaccag cgtggttatg gccgcggaat ccaccaattc gtgaacgcaa
     2341 aagtattccc accagctggc tgaagatcac cttatatgaa ggacgtaatc gccaggtgcg
     2401 ccgcatgacc gcccatgttg gtttccccac gctgcgactg attcgttatg cgatgggtga
     2461 ttacactttg gataaccttg ccaatggcga atggcgagaa gcaacagaat aaggatgcac
     2521 aatgtttaaa ccgcacgtta ccgttgcttg cgtagtgcac gcagagggta aatttttagt
     2581 cgttgaagag acgattaatg gtaaagcgtt atggaaccaa cctgccgggc atctggaagc
     2641 cgatgaaacc ttagtggaag ctgccgcccg tgagctatgg gaagaaaccg gcatcagcgc
     2701 gcaaccgcaa cactttattc gtatgcatca gtggattgcg ccagataaaa cgccgttttt
     2761 gcgtttcctc tttgccattg agcttgagca aatatgcccg acccagcctc atgacagcga
     2821 tatcgactgc tgtcgttggg tcagcgccga agaaatttta aaggcgtcaa atcttcgttc
     2881 gccgctggtg gcggaaagta tccgttgtta tcaaagtggg caacgctatc cgctggagat
     2941 gattggcgat tttaactggc cttttacaaa gggtgtcatc taaaaggctg cgtgatagaa
     3001 tacgccgcct tgaagttcaa tgtcgtgagt gatccaatgt ctgaaaccgc aaaaaaagta
     3061 atcgtcggca tgtccggcgg tgtcgattcc tccgtttctg cctggctgtt gcaacaacag
     3121 ggatatcagg ttgaaggcct gtttatgaag aactgggaag aagacgacgg tgaggaatat
     3181 tgcacagcgg cagcggatct ggctgatgcc caggctgtct gcgacaagct cggcattgaa
     3241 ctgcacaccg ttaactttgc tgccgagtac tgggacaatg tcttcgaact gttccttgcc
     3301 gaatataaag ccggtcgcac gccgaatccg gatattctgt gcaacaaaga gatcaaattt
     3361 aaagctttcc tcgaatttgc cgccgaagat ttaggtgccg attatatcgc taccggtcat
     3421 tacgtacgtc gggccgatgt cgatggcaag agccgcctgc tgcgtggtct ggacagcaat
     3481 aaagaccaga gctacttcct ttatacgctc agccatgagc agattgcgca aagcctgttc
     3541 ccggtcggcg aactggaaaa accgcaggtg cgtaagattg ctgaagatct tggtctggtc
     3601 accgcgaaga aaaaagactc taccggcatt tgcttcattg gcgaacgtaa attccgcgag
     3661 ttcctgggcc gttatctccc ggggcaaccg ggcaaaatca ttaccgtcga tggcgatgaa
     3721 attggtgagc accaggggct gatgtatcac actctcggtc agcgtaaagg tctgggtatc
     3781 ggtggcacca aagaaggcac cgaagaacct tggtatgtgg tggacaaaga cgtcgaaaac
     3841 aacattctgg ttgtcgctca gggccatgaa cacccgcggc tgatgtctgt cgggttgatt
     3901 gcccagcagt tgcactgggt cgatcgcgaa ccattcaccg gcactatgcg ttgcacggta
     3961 aaaacccgct atcgccagac cgatatccct tgcaccgtca aggcgctgga cgatgatcgc
     4021 attgaagtga ttttcgatga accggttgcc gccgtgacgc cgggccagtc tgccgtcttc
     4081 tataacggtg aagtgtgcct cggtggcggt attattgagc agcgtctgcc gctgccggtc
     4141 tgattattat ctttacttaa aacaaggaag cagtgaacgt ggcaaagaat tactatgaca
     4201 tcaccctcgc cctggccggt atttgtcagt cggcacgcct ggtgcaacaa ctcgctcacc
     4261 aggggcattg tgatgccgat gcgctacacg tctcactcaa cagtattatt gatatgaacc
     4321 ccagctcgac gctggcggtt tttggcggta gcgaagccaa cctgcgcgtc gggctggaaa
     4381 ccctgctcgg cgtgctcaat gccagcagtc gccagggctt aaacgccgaa ttaacccgct
     4441 acacactcag cttgatggtg cttgagcgca aactctcctc agcgaaaggc gcgctcgaca
     4501 ctctgggcaa ccggatcaac ggcctgcaac gccagctcga acacttcgat ttacagtccg
     4561 aaacgctgat gagcgcgatg gctgctatct atgttgatgt gatcagcccg cttggcccac
     4621 gcattcaggt caccggttcc cctgctgtac tgcaaagccc acaagtgcag gcgaaagttc
     4681 gcgcaaccct gctggcaggc attcgcgccg ccgtgctctg gcaccaggtc ggcggcggac
     4741 gtctgcaact gatgttttct cgtaatcgcc tgaccactca ggcaaaacaa attcttgctc
     4801 atttaacccc ggagttgtga tctatggaat tatcctcact gaccgccgtt tcccctgtcg
     4861 atggacgcta cggcgataaa gtcagcgcgc tgcgcgggat tttcagcgaa tatggtttgc
     4921 tgaaattccg tgtacaagtt gaagtacgtt ggctgcaaaa actggccgcg cacgcagcga
     4981 tcaaggaagt tcctgctttt gctgccgacg caatcggtta ccttgatgca attgtcgcca
     5041 atttcagcga agaagatgcc gcacgcatca aaaccatcga gcgtaccact aaccacgacg
     5101 ttaaagcggt tgagtatttc ctgaaagaaa aagtggcgga gatcccggaa ctgcacgcgg
     5161 tttctgaatt catccacttt gcctgtactt cggaagatat caataacctc tcccacgcat
     5221 taatgctgaa aaccgcgcgt gatgaagtga tcctgccgta ctggcgtcaa ctgattgatg
     5281 gcattaaaga tctcgccgtt cagtaccgcg atatcccgct gctgtctcgt acccacggtc
     5341 agccagccac gccgtcaacc atcggtaaag agatggcaaa cgtcgcctac cgtatggagc
     5401 gccagtaccg ccagcttaac caggtggaga tcctcggcaa aatcaacggc gcggtcggta
     5461 actataacgc ccacatcgcc gcttacccgg aagttgactg gcatcagttc agcgaagagt
     5521 tcgtcacctc gctgggtatt caatggaacc cgtacactac ccagattgaa ccgcacgact
     5581 acatcgccga gctgtttgat tgcgttgcac gcttcaacac cattctgatc gactttgatc
     5641 gtgacgtctg gggttatatc gcccttaacc acttcaaaca gaaaaccatt gctggtgaga
     5701 ttggttcttc caccatgccg cataaagtta acccgatcga cttcgagaac tccgaaggga
     5761 atctgggcct ttccaacgcg gtattacagc atctggcaag caaactgccg gtttcccgct
     5821 ggcagcgtga cctgaccgac tccaccgtgc tgcgtaacct cggcgtgggt atcggttatg
     5881 cgctgattgc gtatcaatcc accctgaaag gcgtgagcaa actggaagtg aaccgtgacc
     5941 atctgctgga tgagctggat cacaactggg aagtgctggc tgagccaatc cagacagtta
     6001 tgcgtcgcta tggcatcgaa aaaccgtacg agaagctgaa agagctgact cgtggtaagc
     6061 gcgttgacgc cgaaggcatg aagcagttta tcgacggtct ggcgttgcca gaagaagaga
     6121 aagcccgcct gaaagcgatg acgccggcaa actacattgg tcgcgccatc accatggttg
     6181 atgagctgaa ataaactcgt atcagtgccg gatggcgatg ctgtccggcc tgcttattaa
     6241 gattatccgc tttttatttt ttcactttac ctcccctccc cgctggttta tttaatgttt
     6301 acccccataa ccacataatc gcgttacact attttaataa ttaagacagg gagaaataaa
     6361 aatgcgcgta ctggttgttg aagacaatgc gttgttacgt caccacctta aagttcagat
     6421 tcaggatgct ggtcatcagg tcgatgatgc agaagatgcc aaagaagccg attattatct
     6481 caatgaacat ttaccggata ttgcgattgt cgatctcgga ttgccagacg aggacggtct
     6541 gtcactgatt cgccgctggc gtagcaatga tgtttcactg ccgattctgg tattaaccgc
     6601 ccgtgaaagc tggcaggaca aagtcgaagt attaagtgct ggtgctgatg attacgtgac
     6661 taaaccattt catattgaag aggtgatggc gcgaatgcag gcattaatgc ggcgtaatag
     6721 cggtctggct tcacaggtca tttcgctacc accgtttcag gttgatctct ctcgccgtga
     6781 attatctatt aatgacgaag tgatcaaact gaccgcgttc gaatacacca ttatggaaac
     6841 gttgatacgc aataatggca aagtggtgag caaagattcg ttaatgctcc aactctatcc
     6901 ggatgcggag ctgcgggaaa gccataccat tgatgtactg atgggacgtc tgcgcaaaaa
     6961 aattcaggca caatatccgc aagaagtgat tactaccgtt cgcggccagg gctatttgtt
     7021 cgaattgcgc tgatgaaaaa attactgcgt ctttttttcc cgctctcgct gcgggtacgt
     7081 tttctgttgg caacggctgc ggtagtactg gtgctttcgc ttgcctacgg aatggtcgcg
     7141 ctgatcggtt atagcgtcag tttcgataaa actacgtttc ggctgttacg tggcgagagc
     7201 aatctgttct atacccttgc gaagtgggaa aacaataagt tgcatgtcga gttacccgaa
     7261 aatatcgaca agcaaagccc caccatgacg ctaatttatg atgagaacgg gcagctttta
     7321 tgggcgcaac gtgacgtgcc ctggctgatg aagatgatcc agcctgactg gctgaaatcg
     7381 aatggttttc atgaaattga agcggatgtt aacgatacca gcctcttgct gagtggagat
     7441 cattcgatac agcaacagtt gcaggaagtg cgggaagatg atgacgacgc ggagatgacc
     7501 cactcggtgg cagtaaacgt ctacccggca acatcgcgga tgccaaaatt aaccattgtg
     7561 gtggtggata ccattccggt ggagctaaaa agttcctata tggtctggag ctggtttatc
     7621 tatgtgctct cagccaatct gctgttagtg atcccgctgc tgtgggtcgc cgcctggtgg
     7681 agtttacgcc ccatcgaagc cctggcaaaa gaagtccgcg aactggaaga acataaccgc
     7741 gaattgctca atccagccac aacgcgagaa ctgaccagtc tggtacgaaa cctgaaccga
     7801 ttgttaaaaa gtgaacgcga acgttacgac aaataccgta cgacgctcac cgacctgacc
     7861 catagtctga aaacgccact ggcggtgctg caaagtacgc tgcgttctct gcgtagtgaa
     7921 aagatgagcg tcagtgatgc tgagccggta atgctggagc aaatcagccg catttcacag
     7981 caaattggct actacctgca tcgtgccagt atgcgcggcg ggacattgct cagccgcgag
     8041 ctgcatccgg tcgccccact gctggacaat ctcacctcag cgctgaacaa agtgtatcaa
     8101 cgcaaagggg tcaatatctc tctcgatatt tcgccagaga tcagctttgt cggtgagcag
     8161 aacgattttg tcgaggtgat gggcaacgtg ctggataatg cctgtaaata ttgcctcgag
     8221 tttgtcgaaa tttctgcaag gcaaaccgac gagcatctct atattgtggt cgaggatgat
     8281 ggccccggta ttccattaag caagcgagag gtcattttcg accgtggtca acgggttgat
     8341 actttacgcc ctgggcaagg tgtagggctg gcggtagccc gcgaaatcac cgagcaatat
     8401 gagggtaaaa tcgtcgccgg agagagcatg ctgggcggtg cgcggatgga ggtgattttt
     8461 ggtcgccagc attctgcgcc gaaagatgaa taaatatgtc catacttcac gcattacgtt
     8521 aagcatccgt tataatcggt tgcagatacc agcctgtgga tgcttaacat ggaataccaa
     8581 ctcactctta actggcccga ttttcttgaa cgtcactggc agaaacgccc ggtggtgtta
     8641 aaacgcggct ttaataattt tattgacccg atctctccag acgagttggc gggtctggcg
     8701 atggaaagcg aagttgacag tcgactggtc agtcaccagg atggcaaatg gcaggtcagc
     8761 cacggcccgt tcgaaagcta cgatcatctc ggtgaaacca actggtcatt actggtacag
     8821 gcagtgaacc actggcatga gccgaccgcc gcgctgatgc gaccgttccg tgaactaccg
     8881 gactggcgta ttgatgatct gatgatttct ttttctgtac ctggcggcgg cgtcggcccg
     8941 catctcgatc agtacgacgt gtttatcatt cagggtaccg gacgtcgtcg ctggcgagtg
     9001 ggcgaaaagc tgcaaatgaa acagcactgc ccacatccgg atctgttaca ggtcgatccg
     9061 ttcgaagcca tcatcgatga agagctggag cctggtgata ttctttatat tccgccagga
     9121 ttcccgcatg aaggctacgc gctggaaaat gcgatgaact attccgtggg ctttcgcgcg
     9181 ccaaatacgc gggaactgat tagtggattt gccgattatg tgctgcaacg tgaactgggc
     9241 ggcaactact acagcgatcc tgatgttcca cctcgcgctc atcctgcgga tgttctgcct
     9301 caagagatgg ataaactgcg tgagatgatg ctcgaattga tcaaccagcc ggaacacttt
     9361 aagcaatggt ttggcgagtt tatatcccag tcacgtcatg aactggatat cgcgccgcca
     9421 gaaccgcctt atcagccaga tgaaatctac gatgcgctga aacaaggtga tgtgctggtg
     9481 cgcctgggtg gtctgcgcgt attgcgcatt ggcgacgacg tgtatgccaa tggtgagaag
     9541 atcgattccc cgcaccgtcc ggcactggat gcactcgcca gcaacattgc gctgactgcg
     9601 gagaattttg gcgatgcgct ggaagatccg tcattcctcg cgatgctcgc ggcgctggtc
     9661 aatagcgggt attggttctt cgaagggtaa gtttggattt aagccggatg cggcatcaca
     9721 tgccgcatcc cttttcgctt acttccgttg cgccgttaac tcggcaatac ggacgatcac
     9781 ctgcaccgct ttttccatac cttccagagt cacaaactca tgcttaccat gatagttgta
     9841 accgccagtg aacaagttcg ggcacggtaa tcccataaac gacaactgcg cgccgtcggt
     9901 accaccgcgg atcggtttca gttccggttc aatatcgcag tcacgcatcg cctgctgggc
     9961 gatatcgaga atatgcggat gctcaaccac tttctctcgc atattgtagt aactgtcttc
    10021 aatcaccagt tcaatgtagc aatcaggatg taaccctttg cccacttttt tggcgatctc
    10081 catcatttta cgtttacgcg cttcaaactg tttacggtcg aaatcacgga tgatgtagtg
    10141 catatcggcc cgttcaacgg tgcctttcat gctcgccagg tgatagaaac cttcatagcc
    10201 ttctgtcatt tccgggcttt catccgccgg aacttccgca tgaatacgtg ccgccagcga
    10261 cagcgcattt accatcactc ctttcgccgt gcccggatga acattgttac cgacaatttt
    10321 gatattgacc gatgcggcgt tgaagttttc aaactccagt tcgcctacgc caccaccgtc
    10381 aacagtgtaa gcccagcggg catcgaaggc atcaacatca aaatgtttcg cccctttgcc
    10441 cacttcttca tccggggtaa aggcgacgcg aatatcacca tgcggaatgt ttttctgttg
    10501 caataccgcc agcgcggtca tgatttctgc aatacctgct ttgtcatcgg cacctaacaa
    10561 ggttttacca tcggtggtaa tcaacgtctg acccagtagc tgatgcagca ccgggaacat
    10621 aaccggtgat aaaacttcat cgccgatacc cagcgcaata tcgccaccgc gatagttttc
    10681 aacaatttgc ggattaacat ttttgccgct gcaatccggt gaggtatcca catgagaaat
    10741 aaagccaatc gccgggatat cgccagggac gttagccggt aacgtcgcca tcaaagtgcc
    10801 cttctcactt aaggtcacat tgataagccc catttcttcg agctgctctt tcagcagatg
    10861 caataacttc cattggcctt ccgtgctggg aacctgtctc acccctgctt ttgattgggt
    10921 atccagagat acgtagttca aaaatcgctc aagtagttta tccatgtagt caccctcact
    10981 ttttgtgaca acattattaa gaagcaagaa aagacaaata ttgcgtcagg tcacttttac
    11041 ccctgcaagc gggaatattt atcagcatta ccttaatgaa tataaagcta agccagtaat
    11101 tcacaacaaa gattggcgat tcaagcggtt tgccgtagaa ttaccgccct cattaagagt
    11161 caccaaggtg gttaaccaca aaccccgcat cggtaagcca tccgttgcgt ttacatggga
    11221 cagagtaaaa aattgaataa acaaccgagt tcgctttcac cgctggtgca attggcggga
    11281 attcgcaaat gctttgatgg taaagaggtc attccccagc tggatctgac tatcaacaat
    11341 ggcgagttcc tcacgctgct tggcccttct ggctgcggta aaacaaccgt tcttcgcctg
    11401 attgcaggtc tggaaactgt tgattccgga cgcatcatgc tggataacga ggacatcacc
    11461 cacgttccgg cggaaaaccg ctatgtgaac actgttttcc aaagctacgc acttttcccc
    11521 cacatgaccg tgttcgaaaa tgtggccttt gggttgcgca tgcaaaaaac ccccgctgct
    11581 gaaattacgc cccgcgtgat ggaagccctg cggatggtgc aactggaaac cttcgctcaa
    11641 cgtaaaccgc atcagctctc tggtggtcaa cagcaacgcg tcgccattgc tcgcgcggtg
    11701 gttaacaagc ctcgtctgtt gttgctggat gagtcgctct cagcgctgga ttacaaactg
    11761 cgtaagcaaa tgcagaacga gctgaaagcg ttacagcgta agcttggtat taccttcgtc
    11821 tttgtcactc acgatcagga agaagcgctc accatgtcag acaggattgt ggtgatgcgc
    11881 gagggtcgta ttgagcaaga tggtacaccg cgcgaaattt acgaagagcc gaaaaacctg
    11941 tttgttgccg gcttcattgg cgaaatcaat atgtttaacg ccactgtcat cgaacgtctc
    12001 gacgagcagc gcgtacgcgc caacgttgaa ggccgcgaat gtaatatcta cgttaacttc
    12061 gccgttgaac cggggcaaaa actgcatgtt ctgctgcgcc cggaagactt acgtgttgaa
    12121 gagattaacg acgacaacca cgctgaaggg ctgattggtt acgttcgtga gcgtaactac
    12181 aaaggcatga cgctggagtc ggttgttgaa ctggaaaatg gcaagatggt gatggtcagc
    12241 gaattcttca atgaagacga ccctgacttt gaccactctc tcgaccaaaa aatggccatt
    12301 aattgggtag aaagctggga ggtcgtactg gctgatgaag aacacaagta agttccagaa
    12361 tgtagtgatt gtcactattg tcggttggct tgtgttgttt gtctttctgc ccaacctgat
    12421 gatcattggc accagctttt tgacccgcga cgacgccagt ttcgtcaaaa tggtctttac
    12481 gctggataac tacacgcgtc tgctcgatcc gctctatttt gaagtgctat tgcactcgct
    12541 gaatatggcg ctgatcgcca ccctcgcctg cctggtgctg ggctacccgt ttgcctggtt
    12601 tctggcgaag ctgccacaca aggtgcgtcc gctgctgctg tttctgctga ttgttccctt
    12661 ctggaccaac tcactgattc gcatctacgg gctgaaaatt ttcctcagca ccaaaggcta
    12721 tctcaacgag tttttgctct ggctgggcgt tatcgacaca ccaattcgta tcatgttcac
    12781 gcccagtgcg gtgattatcg gtctggttta cattctgcta ccgtttatgg tgatgccgct
    12841 gtactccagt atcgaaaaac tggataagcc gttgcttgag gcggcgcgcg atctcggtgc
    12901 cagcaagtta cagactttta tccgtatcat tattccactg acgatgccgg gaattattgc
    12961 cggatgtctg ctggtgatgc tgccagcgat gggcctgttc tatgtatccg acctgatggg
    13021 cggtgcgaaa aacctgctga tcggtaacgt catcaaggtc cagttcctca atattcgtga
    13081 ctggccgttt ggtgcagcca ccagcattac gctgactatc gtaatgggcc tgatgttgct
    13141 ggtttactgg cgcgcttctc gtctgctgaa taaaaaggtg gaactcgaat gatcggtcga
    13201 ctgcttcgcg gcggttttat gaccgctatc tacgcgtacc tgtatatccc aatcattatt
    13261 ttgattgtga actcctttaa cagctcgcgc tttggcatca actggcaggg ttttaccacc
    13321 aaatggtata gcctgctgat gaacaacgac agcctgttac aggcagcgca gcattcacta
    13381 acaatggcgg tgttttcggc gacatttgct acgcttatcg gttcactgac ggcagttgcg
    13441 ctgtaccgtt atcgctttcg tggtaagccg ttcgttagcg gaatgctgtt tgtggtgatg
    13501 atgtcaccag atatcgtgat ggcgatttct ctgctggtgc tgtttatgct gctgggtatt
    13561 cagcttggct tctggtcgct gctgttctcg catatcacct tctgcctgcc gtttgtggtg
    13621 gtgacggtgt attcgcgcct gaaaggtttt gacgtgcgga tgctggaagc ggcgaaagat
    13681 ctcggtgcca gtgaatttac cattctgcga aaaatcattc tgccactggc aatgccagcg
    13741 gtggcggcgg gctgggtgtt aagctttacc ctgtcgatgg acgatgtggt agtttcttcg
    13801 ttcgtcaccg gaccaagtta tgaaattctg ccgttaaaaa tttattcgat ggtcaaagtc
    13861 ggcgtatcgc cggaagttaa cgcgctggca accatattac tggtgctgtc gctggtgatg
    13921 gttattgcca gccagcttat tgctcgtgat aaaacgaaag gtaacacagg ggacgttaaa
    13981 tgaaaaaatg gtcacgccac ctgctcgcgg cgggtgctct ggcactgggc atgagcgccg
    14041 ctcacgccga tgacaacaac acgctgtatt tctacaactg gaccgagtac gtgccgccag
    14101 gactgcttga acagttcacc aaagaaaccg gtattaaggc tatctattcg acttacgagt
    14161 cgaacgaaac catgtacgcc aagctgaaaa cgtacaaaga cggtgcctat gacctggtgg
    14221 ttccttcaac ctattacgtc gataaaatgc gtaaagaagg gatgatccag aagatcgaca
    14281 agtcgaagtt aacgaacttc agcaatctcg atccagacat gctcaacaag ccgtttgacc
    14341 cgaataacga ctactccatt ccgtatatct ggggtgcgac ggcgattggc gttaacggtg
    14401 atgcggtgga tcctaaatct gtcaccagtt gggcagatct gtggaagcca gagtacaaag
    14461 gtagcctgct gctgaccgac gatgcccgtg aagtgttcca gatggcgctg cgcaaactgg
    14521 gctactccgg taacaccacc gatccgaaag agattgaagc tgcatataac gagctgaaaa
    14581 aactgatgcc aaacgtcgca gcgtttaact ccgataaccc ggctaacccg tacatggaag
    14641 gcgaagttaa cctcggcatg atctggaacg gctctgcttt cgttgcacgc caggcgggta
    14701 cgccaattga cgtggtgtgg ccgaaagaag gcggcatttt ctggatggac agcctggcga
    14761 tcccggcaaa tgccaaaaac aaagaaggcg cgctgaaatt gatcaacttc ctgctgcgcc
    14821 cggatgtggc aaaacaggtt gccgaaacta tcggttatcc aacgccaaac cttgcggcgc
    14881 gtaagctgtt aagcccggaa gtggcgaacg ataaaacgct ctacccggat gctgaaacca
    14941 ttaaaaatgg tgaatggcag aatgacgttg gcgcagccag cagcatttat gaagagtatt
    15001 atcagaagct gaaagcagga cgttaattca gcaaacgggc ggtggtatta ccgcccgttt
    15061 tttagttagc aaggatgtgg ctaatgagtc aggacagtaa agttttatcc cgctctttcg
    15121 tcggtattgt ggtccccttg taggcctgat aagcgtagcg catcaggcaa tgcttcctgc
    15181 tttataatcc cttcagcaac ttttcaacaa actcaggcac cacctggctt gccgggccgt
    15241 aatatttctc ggcaaattca ttaccgacct gacttggttc gagattcagt tccacggtgt
    15301 gcgcgccatg cagtttcgct tcgtgaacaa acccagccgc cggataaaca tgcccggaag
    15361 taccaatggc aatgaaaata tcagccatcg acaacgccat ataaatttca tccatgccga
    15421 gtggcatttc gccaaaccaa acgacgtgcg ggcgcaaggg tgccgggaac tggcaacaat
    15481 ggcatttatc ttctggcgta acgtctccgg tccagtcgag aacctgacca ctttgcgaac
    15541 aacgaacttt cagcaattcc ccatgcatat gaatgacatt ggtattacct gcgcgttcat
    15601 gcaggttgtc tatattctgc gttaccagca aaaagcgatc gccgagggcg tcttgcagtt
    15661 tagccagcgc aagatgcgcg gcgttaggct gaatttctgg ctgctgcagc tgtcgacgac
    15721 gggcgttata aaacgcttgc accagttcag gatcgcgatc gaaaccttcc ggcgttgcca
    15781 catcttcaac ccgatgttct tcccacaggc catctgcggc gcgaaaggta cgaatacctg
    15841 attccgcaga aattcctgcc cctgtcagta cgagtactct tggtttttcc attgcttccg
    15901 gcaccacttt atctctgaaa aaaatacgct gacgcaaacg ctcgcgcagg cggcgtttat
    15961 ttttacgaaa acgacttaac cgatgacccc gacgcgacag catagcaacc tctgttgttt
    16021 aatcggttag atgtaggaag gccgcaccac gcattcctcc cgcatcaccg tggcgcgcgc
    16081 gttcaatgcg cggaacacga gctacaggta agagatgacg aggcagcctg tccgccagtt
    16141 gcgttgtgat tgccgggaaa ttcgataagc caccaccaat gacgaccagg tcagagtcaa
    16201 cgatggtcag gatatttccc agacaaaccg ctaataaatc cagataacgc tcaacgtgcg
    16261 cccttgcctg ctcatcgcct tgatcataaa gcgcaataat ttcgggagcc tgcaacggtt
    16321 gatgataata gtgttgccag agccacgcaa agccccgacc tgatagataa ttttcgatac
    16381 aaccaatttg accacagccg cagcggcgta acgggaaatc cagccccatc atggttaacg
    16441 catcaaccgg cagacgcata tggccaaact cgccggtaat gtagctttta ccggtaatcg
    16501 gtttaccgtt gaaaatcagc ccgccgccaa cgccggtgcc gagaattaac cccatcacca
    16561 gtggatattg cgtaaattca tcatcccagg cttctgaaag ggcaaaacag ttggcatcgt
    16621 tatcaaggcg tacatcgcga tcaagacgtg cgctcaggtc ggcacgcagc ggtttaccgc
    16681 tggcagcggg gacattggcg gcatacagcg taccatcttc tgtttccggc attcccggaa
    16741 taccgatgcc aacagaacct ttacagccaa aacgttggtc agcttcggct accagctcgc
    16801 atactgcatc taaaaatgcg tcatagctgt cacgcggtgt cggcacccgc ttttcccact
    16861 gcaactgccg accgctatca aacacgccaa gcgcaatttt tgttccacca atatcaaacc
    16921 cgtaatacat tgccgctcct tgttttaatg tactgccttt actggccgct aaggactcgc
    16981 gcagggtcaa tattactggc gcgccgcgcc ggataccaac ttgccaaaag actcagcaac
    17041 aatgctgtga ccagtacgta gaagacgtcc agccagtgca attccgatgg caggaagtca
    17101 ataaaataga tatcgctgga gaggaactga tgaccgatca gcttttcaat ccgctcaata
    17161 atcggggtaa gttgcagtga aacaactacg ccgataatca caccacacag gctaccgaac
    17221 agccctgcca gcaatccata ccagacaaag atggcgcgaa ttaaaccatc tttcgccccc
    17281 agcgttctta atactgcgat atcgccactc ttgtctttca ccgccatcac taaggtggag
    17341 acgatgttga aacaggccac gccaatcacc agtaccatcg ccagatacat aatggcgcgg
    17401 atcatctgga tatcgcgata catatagccg taagtaccaa tccaactttt aatataaaca
    17461 tagctgttgg tcacttcacc cgcatcgcgt accagcttat tggcgttgaa aacatccgtc
    17521 attttaaggg caatacctga cacgctggaa cccatatcaa gatattgttg ggcatccgcc
    17581 agcgggatca tggcaaaact gtgatcgagt tgaccactca actgcaaaat accggcaatg
    17641 tgcaaacgca cacgttttgg ctgcatcagt ttatgctcgg gattcgagtt ggggatcatg
    17701 atcgacaccc aatcgccctg cttcactttc agcgcatccg ccacgccttt gccgatgata
    17761 atttgctgtt cgcccgcttt aaaattgcgc caggcatccc cctgaacaaa cgagggtaat
    17821 gcgctcagac gctgttcctg ttgcgggtta acgcccttca cctgaattgc gcgcagattg
    17881 gtaccacttt ctaccagtcc ggtgaaattg atatacggcg cagcggcggc aatacccggc
    17941 actttttgca cgttatccag tgcttcctgc cagttagtcc atggctgatt taccgcctca
    18001 atttcaccgt gcggtaccac agcaagaatg cggttattca gttcgcgttc aaagccgttc
    18061 atcgcgctta agccgacgat caataccgcc acgccgaggg caatgccaat ggtagaaatg
    18121 acagagatta gcgacaccat gccgccgcgt cgccgtccgc gactaaaacg caggccaatc
    18181 aataacgata aaggcatcgc cattactccg cccccatcag gctcagttcc gccgtcagac
    18241 gtccatcacg catttccagt tggcggctca tacgtttcgc cagttgcagg tcgtgagtaa
    18301 ccaccaggaa ggcggtgccc tgcaagcgat tcaattcccc aagcaactgg aagatgctgt
    18361 cggcgttacg cgcatcgagg ttaccggtag gctcatccgc aagtaccaga cgcggattat
    18421 tgaccagcgc acgggcaatc gccacacgct ggcgttcgcc gccagaaagt tcagatgggc
    18481 ggtgattcgc acgatgctcc agccccaccg cttttaacat ctccagcgca cggctgttga
    18541 tttcagcggg ctttttcttg ccaatcagca gtggcatagc cacgttttcc agggcagtaa
    18601 aatccggcag caggtggtga aactgataaa taaagcccag cttctggttg cgcagttctg
    18661 ctttcgccgc cgaagacagt ttgctcatcg gttgaccgtt aaagatcaca tcgccggagg
    18721 ttggtgtatc cagcccgccc agcaggtgca gcaaggtact tttaccggaa ccagagctac
    18781 cgacgatcgc catcatttca ccttcgccga cgctgaaact gacattgtgc agtacatcgg
    18841 tttgcacact gccttcctga tagcgtttgc acaggttgtc gcattgcaac aggatcttat
    18901 tcataacgta aagcctcagc gggttgagtg gcggcagcgc gccatgaagg gtaaagcgta
    18961 gacagcagcg cgatagccat cgccaccagc gcaataacaa tgacctgtaa aggttcgata
    19021 gcgaccggca gcgccgcgcc atcaagcagg acgccgatta tcggcatcag attatttaac
    19081 tggctggcaa gcagtgcgcc aagcgccgct ccgaggatcg caccgataat cccggcgctg
    19141 gccccttgca ccataaagac catcatgatt tgtcgcggag ttaagccttg cgtttgcagg
    19201 atcgcgactt cgccctgctt ctccattacc atcagcccta gtgaggtaat aatgttaaac
    19261 gccgcaacgg cgacaatcag gctcagcagc aagcccatca tatttttttc catgcgtacg
    19321 gcctggaaca gctcgccttt acggtcacgc cagtcctgcc atttgctgcc ttcaggcagt
    19381 ttttgctgac ttaaagagtc aactttcagc ggctcatcca gccacaaacg ccagccggta
    19441 atattgcctg ccggataacg catcagacgc gaggcatcct caatattcac cagcatttca
    19501 tagccatcga cttcactgtt ggcggcgaaa gtgccaatca cattgaacag gcgctggctt
    19561 ggaatacgcc ccatcggcgt gaactggctg gcagatggta ccatcacgcg gatttgatca
    19621 ccgcgattaa cgcctagctg tgaggcaagt tgttcgccga ggatgacatt atatttcccc
    19681 ggctcgaggt cagtttgttt cacattgacc agatacggcg taagtggatc tttttgcgcc
    19741 gggtcgatac cgagcatcac cccgaccgcc acgctgcgcg cgctttgcag taccacatca
    19801 ccggtagtaa taggtgcgac gcgattaacg ccgtccagtt tgactgccgt ttccgggagt
    19861 tgctgcgggt taagagagcc atgctcagaa gagagaattg cctgtggcat caggccaagg
    19921 atgttgtttt gcagctcgcg ctcaaagccg ttcatcactg acaatactgt gaccagcgcc
    19981 atcaccccga gggtaatgcc gatggtagaa agccaggaga cgaaacgacc gaagcgatcc
    20041 gctgcacgcc cacgcatgta acgcaggcca ataaatagag cgacaggttg gtacatgaaa
    20101 tccgtctggt tgctgtagca aagacacgag tatataagcg aaagcaagat gggtaaacgt
    20161 ctgaactgta agtgatcgtc agggctaatc tgaaaagccc ccgtaaatca atcggctaaa
    20221 ttgccatgtt ttatcatcaa tcttgccgcc tgatcttaat ctgaaaaatc atccaataca
    20281 gacagatacc cattacacct cgggtcataa cccgcaggat caaccgcatg taaattaaaa
    20341 aagagcgtgt tgtcctgatg aaacaaaaag agctatggat taaccagatc aaagggttat
    20401 gtatttgtct ggtggtgatt tatcactcgg tcattacctt ttatccgcat atgaccactt
    20461 ttcagcatcc gttatcggaa gtcctgagca aatgctggat ctatttcaat ctttaccttg
    20521 caccctttcg tatgccggtt tttttcttta tatccggcta tttgattcgc cgctatatcg
    20581 acagcgtacc gtggggaaat tgtctcgata aacggatctg gaacatcttc tgggtgctgg
    20641 cgctttgggg cgtggtgcag tggctggcgt taagtgcgct gaaccagtgg ctggcacctg
    20701 agcgcgattt aagcaatgcc tccaatgccg cttatgccga ttccaccggt gagttcctgc
    20761 acgggatgat caccgccagc accagcttgt ggtatctgta tgcgttaatt gtctatttcg
    20821 tggtatgtaa aatttttagc cgtctggcgc tgccactatt cgccttgttt gtactgctga
    20881 gtgtggcggt taatttcgtt cccacgccgt ggtggggaat gaacagtgtg atccgcaatt
    20941 tgccttatta cagccttggc gcatggtttg gcgcaacaat aatgacctgt gttaaagagg
    21001 tgccgttgcg ccgccatctg ctgatggctt ctttgcttac cgttctggcg gtcggtgcct
    21061 ggttgtttac tatctcgctg ctgttgtcgc tggtatcgat tgtggtgatc atgaagctgt
    21121 tttatcagta cgagcaacga ttcggtatgc gctccaccag cctgctgaat gtgattggct
    21181 ccaacaccat tgctatctac accacccatc gcattctggt tgaaatattc agcttaactc
    21241 tgcttgcgca aatgaacgca gcacgctggt cgccgcaagt cgaactgaca ctcctgctgg
    21301 tttatccctt tgtcagtttg ttcatctgta ctgttgcggg cttgctggta agaaaacttt
    21361 cacagcgtgc attcagcgat ctgttgttct ccccgccttc actgcccgct gccgtcagtt
    21421 actctcgtta aaccatcgcc ccagccgggg cgattcaatt tgctaaacca tgtcgattac
    21481 ggataatgaa cagcattgct tatcagagat atgcccccat atgttgaggc atatcctaac
    21541 gagaatctga caaccgttat gcctgaacaa tatcgttata cactgcccgt caaagcgggt
    21601 gagcagcgtc tgctgggcga gttaaccggc gcagcctgtg caacgctggt agcggaaatt
    21661 gccgaacgtc acgccggtcc ggtggtactc attgcaccag atatgcaaaa tgctctgcgt
    21721 ttgcatgatg aaatcagcca gttcaccgat cagatggtga tgaatctggc ggactgggaa
    21781 actcttccct acgacagttt ttcgcctcat caggacatta tctcctcgcg cctttccacc
    21841 ctttaccagc taccgacgat gcagcgtggc gtactgattg ttccggtgaa tacgcttatg
    21901 cagcgcgttt gcccacacag ttttctccac ggtcatgcgc tggtgatgaa aaaaggtcag
    21961 cgcctgtcac gagatgcatt acgaacccaa ctggacagcg ccggttatcg ccatgttgac
    22021 caggtgatgg agcacggcga atacgccacg cgcggcgcgt tgctggatct cttcccgatg
    22081 gggagtgagc tgccttatcg tcttgatttc tttgatgatg aaatcgacag cctgcgggtg
    22141 tttgacgtcg acagccagcg cacgctggag gaagtagaag cgatcaatct gctgcccgcg
    22201 cacgaatttc cgaccgataa agcggcaatt gaactgttcc gcagccagtg gcgcgatacc
    22261 ttcgaagtga agcgcgatcc ggaacatatt taccagcaag tgagtaaagg cacattacct
    22321 gccgggatcg agtactggca gccattgttc ttcagcgaac cactgccgcc gctgttcagt
    22381 tatttccctg ccaatacctt gctcgtgaat actggcgatc tggaaaccag tgccgaacgt
    22441 ttccaggctg acacgctggc gcgttttgag aatcgcggcg tcgatccgat gcgcccgctg
    22501 ttgccaccac aatcgctctg gctgcgggtg gacgagctct tctcagagct gaaaaactgg
    22561 ccccgggtgc agctaaaaac tgaacattta ccgacaaaag ccgcgaatgc caatttaggt
    22621 ttccagaaac tgccagacct ggccgttcag gcacaacaaa aagcgccgct ggatgcgctg
    22681 cgtaagttcc tcgagacttt cgacggtccg gtggtgttct cggtagaaag tgaaggtcgc
    22741 cgtgaagcgc tgggtgaact gctcgcacga attaaaattg ctccgcaacg cattatgcgt
    22801 cttgatgaag ccagcgaccg tgggcgttat ctgatgattg gcgctgccga acatggtttt
    22861 gtcgataagg tgcgtaatct ggcgctgatc tgcgaaagcg atctgctcgg tgaacgtgtt
    22921 gcccgccgtc gtcaggattc tcgccgcacc atcaaccccg atacactgat ccgtaacctt
    22981 gcggagctgc atattggtca gccggtggtc catctggagc acggtgtcgg gcgctatgcc
    23041 ggaatgacca cgctcgaagc gggcggcatt actggcgagt atttgatgct cacctatgcc
    23101 aacgacgcca aactgtatgt tccggtgtcg tcactgcatc tgattagccg ttacgcaggt
    23161 ggcgcggaag aaaacgcccc gctgcataaa cttggcggcg atgcgtggtc acgcgcgcgg
    23221 cagaaagcgg cggaaaaagt gcgtgatgtg gcggcggaat tgctggatat ctacgcgcaa
    23281 cgcgccgcca aagagggctt cgcgtttaaa cacgatcgtg agcagtatca gttgttctgc
    23341 gacagcttcc cgtttgaaac cacgccggat caggcgcagg ccattaatgc ggtacttagc
    23401 gacatgtgtc agccgctggc aatggatcgt ctggtgtgcg gcgatgttgg ctttggtaaa
    23461 acagaagtgg cgatgcgcgc cgctttcctg gcagtagata accacaagca ggtggcggtg
    23521 ttggtgccta ccacccttct cgcgcagcag cattacgaca acttccgcga ccgtttcgcc
    23581 aactggccgg tacgtatcga aatgatctcc cgtttccgca gcgccaaaga gcagacgcaa
    23641 atccttgcgg aagtggcgga agggaaaatc gatattctga tcggtacgca caaactgctg
    23701 caaagtgacg tcaagtttaa agatttaggc ctgctgattg tcgatgaaga acaccgcttc
    23761 ggggtgcgtc ataaagagcg cattaaagcg atgcgcgcga acgtggatat tctgacgctt
    23821 actgcaacgc cgatcccacg cacgctgaat atggcaatga gcggaatgcg tgacctgtcg
    23881 attatcgcca cgccgcccgc ccgtcgtctg gcagttaaaa cctttgtccg tgagtatgac
    23941 agcctggtgg tccgggaggc gatcctgcgt gaaattttgc gcggggggca ggtttattat
    24001 ctctacaatg atgtggaaaa catccagaaa gctgccgaac ggctggcaga actggtgcca
    24061 gaagcgcgga tcgccatcgg tcacgggcag atgcgcgagc gcgaactgga acgggtgatg
    24121 aatgatttcc atcatcaacg tttcaacgtg ctggtttgta caaccattat cgaaaccggg
    24181 atcgacatcc cgacagccaa caccattatc attgaacgcg cggatcactt cggtctggcg
    24241 cagctgcacc agttacgcgg tcgcgtcgga cgttcgcatc atcaggcata tgcatggttg
    24301 ctgacaccgc atccaaaagc gatgactacc gatgcacaaa aacgtcttga agcgattgcc
    24361 tcgctggaag atctcggggc aggttttgcg ctggcaacgc acgatctgga gattcgcggc
    24421 gcgggtgaac tgcttggcga agaacaaagc ggctcaatgg aaaccatcgg tttctcgctg
    24481 tatatggagt tgctggaaaa cgccgtcgat gcactgaaag ccggacgcga gccgtcgctg
    24541 gaagatctca ccagccagca aacagaagtc gagctgcgga tgccgtcgct attgccagat
    24601 gatttcattc ctgacgtgaa tacgcgtttg tcgttctata aacgtattgc cagcgccaaa
    24661 acggaaaacg aactggaaga gatcaaagtc gagcttatcg atcgcttcgg cctgctgcca
    24721 gatccggcgc gtaccctgct ggatattgcc cgtctgcgcc agcaagcgca gaaactgggg
    24781 atcaggaagc tggaaggtaa tgagaaaggc ggggtgatcg aatttgccga gaagaatcac
    24841 gttaatccgg cctggttgat tggtttgctg caaaaacagc cgcagcatta ccgccttgat
    24901 ggtccgacgc gcctgaaatt tattcaggat ttgagtgagc ggaaaacgcg tatcgaatgg
    24961 gtacgccagt ttatgcgtga actggaagag aacgcgatcg cttaatggta aatcgggggc
    25021 gtttctgcgc ccccatctgc aacatttaca aattctttgc acttccctgc actatccgac
    25081 actgtcacca tccataattc aggcttatct gtttattaca ataaccttat atttattatt
    25141 gatttttggt gatgatcaaa acgcgttttt ctcgctggct aacgtttttt acgttcgccg
    25201 ctgccgtggc gctaccggca aaagccaaca cctagccgct gccgccagcg agcagtcgtc
    25261 tggttggcga aaacaaattt catgtggtgg aaaatgacgg tggttctctg gaagccatcg
    25321 ccaaaaaata taacgtcggc tttctcgctc tgttacaggc taaccccggc gttgatcctt
    25381 acgtaccgcg cgcgggcagc gtgttaacga tcccgttgca aaccctactt ccagacgcgc
    25441 cgcgcgaagg cattgtgatc aacattgcgg agctgcgtct ctattactac ccgccgggta
    25501 aaaattcggt aaccgtgtat ccaataggta ttggtcagtt aggtggtgac acgctgacac
    25561 cgacaatggt gaccaccgtt tcagacaaac gtgcaaaccc aacctggacg ccaacggcaa
    25621 acatccgcgc ccgttataaa gcacagggaa ttgagttgcc tacggtagtg ccggctggac
    25681 cggataaccc aatgggccat catgcgattc gtctggcggc ctatggcggc gtttatttgc
    25741 ttcacggtac gaacgccgat ttcggcattg gcatgcgggt aagttctggc tgtattcgtc
    25801 tgcgggatga cgatatcaaa acactcttta gccaggtcac cccaggcacc aaagtgaata
    25861 tcatcaacac tccgataaaa gtctctgccg aaccaaacgg tgcgcgtctg gttgaagtac
    25921 atcagccgct gtcagagaag attgatgacg atccgcagct gctgccaatt acgctgaata
    25981 gcgcaatgca atcatttaaa gatgcagcac aaactgacgc tgaagtgatg caacatgtga
    26041 tggatgtccg ttccgggatg ccggtggatg tccgccgtca tcaagtgagc ccacaaactc
    26101 tgtaaatcct ggataaaaaa aatccccgcg acacggcatg tcgcggggaa aaaatcagca
    26161 gtagcaggca ttaatgaggg ttaatgctta tttataaatt actgctgttc catggagggt
    26221 attcggaccg gttacagaag taatacggaa agattttgcg cccatctcat ccgctttttg
    26281 cgccagctgc tcttccagcg atcccagatt tgtccccgcg ttagcactga tagtaccgac
    26341 tttttgttgg ccttctggcg ttgactgaac ttcgacagcc gcaaagttgg caaatgacat
    26401 ggagcttaaa atcgccgcag cgatgagggt ttttacgttt ttcataatag tggccttatg
    26461 cagatgaatg acgtcgcaag aaattactta acgatcgata actaaatgat agat
//
LOCUS       DAZPDU010000042        26237 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZPDU010000042 DAZPDU010000000
VERSION     DAZPDU010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26237
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.042"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(58..1080)
                     /locus_tag="ACHURS_001615"
     CDS             complement(58..1080)
                     /locus_tag="ACHURS_001615"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFR1237380.1"
                     /translation="MTVKVIDLFCGAGGLTHGLQLAGLDVVAGIDLEGECRFPYERNN
                     KSKFIEQDIAKVTKEELLRLYGDASVKVLAGCAPCQPFSKYTQGKDKADDKKWPLLYE
                     FERLIREVSPEIVTMENVPDVTKHKVYNDFYNSLLKLGYYVWASKIDCVEYGIPQNRS
                     RHVLLASKLGAIELVKRDDVILKTVRDAIGNLPPLEDGQTDPNDILHRASKLNPINKK
                     RIIHSVPGGTWKDWPKELVAACHMKSSGKGYASVYGRMSWDKPSPTITTLCYGFGNGR
                     FGHPEQHRAISLREAALLQTFPMDYIFVEDKNKFVIRSIGKMIGNAVPVELGKAIGQS
                     IKNHLE"
     gene            complement(1167..3464)
                     /locus_tag="ACHURS_001616"
     CDS             complement(1167..3464)
                     /locus_tag="ACHURS_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFR1237381.1"
                     /translation="MAVKASGRFVPPSAFAAGTGKAFTGAYAWNAPREAVGRERPLTR
                     DEMRQVQGVLSTINRLPYFLRSLFTSRYDYIRRNKSPVHGFYFLTSTFQRRLWPRIER
                     VNQRHEMNTDASMLFLAERDHYARLPGMNDKELKKFAARISSQLFMMYEELSDAWVDA
                     HGEKESLFTDEAQAHLYGHVAGAARAFNISPLYWKKYRKGQMTTRQAYSAIARLFNDE
                     WWISQLKGQRMRWHEALLIAVGEVNKDRSPYASKHAIRDVRARRQANLEFLKSCDLEN
                     RETGERIDLISKVMGSISNPEIRRMELMNTIAGIERYAAAEGDVGMFITLTAPSKYHP
                     TRQVGKGESKTVQLNHGWNDEAFNPKDAQRYLCRIWSLMRTAFKDNDLQVYGLRVVEP
                     HHDGTPHWHMMLFCNPRQRNQIIEIMRRYALKEDGDERGAARNRFQAKHLNRGGAAGY
                     IAKYISKNIDGYALDGQLDNDSGRPLKDTAAAVTAWASTWRIPQFKTVGLPTMGAYRE
                     LRKLPRGVSIADEFDERVEAARAAADSGDFALYISAQGGANVPRDCQTVRVARSPSDE
                     VNEYEEEVERVVGIYAPHLGARHIHITRTTDWRIVPKVPVVEPLTLKSGIAAPRSPVN
                     NCGKLTGGDTSLPAPTPSEHAAAVLNLVDDGVIEWNDPEVVRALRGALKHGLRRPNRQ
                     QRNGSPLKPHEIAPSARLTRSERMQITRIRVDLAQNGIRPQRWELEVLARGATVNYDG
                     KKFTYPLADEWSGFSTVLESNSVKR"
     gene            complement(3454..3729)
                     /locus_tag="ACHURS_001617"
     CDS             complement(3454..3729)
                     /locus_tag="ACHURS_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HFR1237382.1"
                     /translation="MSIRIEIGERYVVTSDSFQFILHEKKRAESGKNAGQEWLAVVGY
                     YPKLSQLVSGLMHHDILTGSAKSFADLNAQVEQLSKRCSEAFGSYGR"
     gene            complement(3726..3950)
                     /locus_tag="ACHURS_001618"
     CDS             complement(3726..3950)
                     /locus_tag="ACHURS_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFR1237383.1"
                     /translation="MPDNIDFIQEQQAELLERQINAARVKHCGASALVCEECDAPIPA
                     ARRAAYPSATRCVSCQSVFEAKNKHYRRTA"
     gene            complement(3953..4252)
                     /locus_tag="ACHURS_001619"
     CDS             complement(3953..4252)
                     /locus_tag="ACHURS_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HFR1237384.1"
                     /translation="MSCSHSVVLLNNALKIAVMKNGDLSLIQLCLDKEKRDITESVIA
                     IYQNELNLLSDVVNLLVKRAVFHKQISSVDELTKLTTELASYCADVSRKLNDKRS"
     gene            complement(4252..4476)
                     /locus_tag="ACHURS_001620"
     CDS             complement(4252..4476)
                     /locus_tag="ACHURS_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="HFR1237385.1"
                     /translation="MHTVSENQCGKYALLLQQARTEAQADAATRFSSHLDAMIRHITK
                     AELSRVEIVELLSQESEKFHNIGLSRGEVL"
     gene            complement(4540..5040)
                     /locus_tag="ACHURS_001621"
     CDS             complement(4540..5040)
                     /locus_tag="ACHURS_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein B"
                     /protein_id="HFR1237386.1"
                     /translation="MTVMTLNLVEKQPAAMRRIIGKHLAVPRWQDTCDYYNQMMERER
                     LTVCFHAQLKQRHATMRFEEMNDVERERLVCAIDELRGAFSKRRQVGASEYAYISFLT
                     VSQRRTLFMHAGLTEKEFNQPYWRINEESCYWRDALFRALRELFSLFEYAPTILTSVK
                     PEQYLH"
     gene            complement(5210..5482)
                     /locus_tag="ACHURS_001622"
     CDS             complement(5210..5482)
                     /locus_tag="ACHURS_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cox family DNA-binding protein"
                     /protein_id="HFR1237387.1"
                     /translation="MDAENYVIQYPLDAVHVDKFADLLGKPKTAVSEMVKANKLPIIE
                     LRDPCKPKARAGEKWVFIPEFNRAVREAFYNRPVEQRDAWLLWMGL"
     gene            5619..5912
                     /locus_tag="ACHURS_001623"
     CDS             5619..5912
                     /locus_tag="ACHURS_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1237388.1"
                     /translation="MKLSEKIKALREAEGLSQSKFCEIIELPLSTLKKYEGGNFEPGG
                     TALLKITMHPTFQKYALWLMTDKTAPDAGQIAPALAHIGPESTESNHSAKRIG"
     gene            5982..6962
                     /locus_tag="ACHURS_001624"
     CDS             5982..6962
                     /locus_tag="ACHURS_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1237389.1"
                     /translation="MAIKKLDDGRYEVDIRPRGRDGKRIRRKFERKAEALAFERYTIA
                     NASQKEWGGQRADRRTLSELLDIWWKYHGQNHEHGTKEFNHLLKTISGIGDIPVSRMS
                     KRALMDYRSMRLRDGISAATINRDMYRLSGMFTKLIQLDEFSGQHPIHGLPPLAEANP
                     EMTFLEKAEIEKLLNVLAGDDLLVALLCLSTGGRWTKVATLKPAQITNCRVTFLKTKN
                     GKKRTVPISEELEKKVKEEASAKLFKVDYEKFCGILRRVKPDIPPNQATHILRHTFAS
                     HFMMNGGNIIALQQILGHASIQQTMAYAHLAPDYLQNAVALNPLKGGVTL"
     gene            complement(7148..7648)
                     /gene="cpxP"
                     /locus_tag="ACHURS_001625"
     CDS             complement(7148..7648)
                     /gene="cpxP"
                     /locus_tag="ACHURS_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFR1237390.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            7798..8496
                     /gene="cpxR"
                     /locus_tag="ACHURS_001626"
     CDS             7798..8496
                     /gene="cpxR"
                     /locus_tag="ACHURS_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFR1237391.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            8493..9866
                     /gene="cpxA"
                     /locus_tag="ACHURS_001627"
     CDS             8493..9866
                     /gene="cpxA"
                     /locus_tag="ACHURS_001627"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFR1237392.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(9972..10646)
                     /gene="yiiM"
                     /locus_tag="ACHURS_001628"
     CDS             complement(9972..10646)
                     /gene="yiiM"
                     /locus_tag="ACHURS_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFR1237393.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(10795..11778)
                     /gene="kdgT"
                     /locus_tag="ACHURS_001629"
     CDS             complement(10795..11778)
                     /gene="kdgT"
                     /locus_tag="ACHURS_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFR1237394.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(12038..12658)
                     /gene="sodA"
                     /locus_tag="ACHURS_001630"
     CDS             complement(12038..12658)
                     /gene="sodA"
                     /locus_tag="ACHURS_001630"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFR1237395.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            12943..13977
                     /gene="rhaT"
                     /locus_tag="ACHURS_001631"
     CDS             12943..13977
                     /gene="rhaT"
                     /locus_tag="ACHURS_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HFR1237396.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWTISALLLPDFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNTGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(13974..14822)
                     /gene="rhaR"
                     /locus_tag="ACHURS_001632"
     CDS             complement(13974..14822)
                     /gene="rhaR"
                     /locus_tag="ACHURS_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFR1237397.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVSFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(14896..15732)
                     /gene="rhaS"
                     /locus_tag="ACHURS_001633"
     CDS             complement(14896..15732)
                     /gene="rhaS"
                     /locus_tag="ACHURS_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFR1237398.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRAHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            16020..17489
                     /gene="rhaB"
                     /locus_tag="ACHURS_001634"
     CDS             16020..17489
                     /gene="rhaB"
                     /locus_tag="ACHURS_001634"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFR1237399.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRSNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACREMAQPLPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQLHSTRQTKELCA"
     gene            17486..18745
                     /gene="rhaA"
                     /locus_tag="ACHURS_001635"
     CDS             17486..18745
                     /gene="rhaA"
                     /locus_tag="ACHURS_001635"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFR1237400.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            18987..19811
                     /gene="rhaD"
                     /locus_tag="ACHURS_001636"
     CDS             18987..19811
                     /gene="rhaD"
                     /locus_tag="ACHURS_001636"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFR1237401.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHGNFHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            19821..20135
                     /gene="rhaM"
                     /locus_tag="ACHURS_001637"
     CDS             19821..20135
                     /gene="rhaM"
                     /locus_tag="ACHURS_001637"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFR1237402.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            20436..20882
                     /locus_tag="ACHURS_001638"
     CDS             20436..20882
                     /locus_tag="ACHURS_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFR1237403.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIHQLQQGDADQVLALLNQTLSS"
     gene            20893..22344
                     /locus_tag="ACHURS_001639"
     CDS             20893..22344
                     /locus_tag="ACHURS_001639"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFR1237404.1"
                     /translation="MESSLCIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            22334..23404
                     /locus_tag="ACHURS_001640"
     CDS             22334..23404
                     /locus_tag="ACHURS_001640"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFR1237405.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GFKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            23404..25152
                     /locus_tag="ACHURS_001641"
     CDS             23404..25152
                     /locus_tag="ACHURS_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HFR1237406.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNFQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGNEIKQIV
                     EQSGMFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELIAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAADIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(25202..>26237)
                     /locus_tag="ACHURS_001642"
     CDS             complement(25202..>26237)
                     /locus_tag="ACHURS_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HFR1237407.1"
                     /translation="RNLLSSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAPEA
                     PVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQNSLARYADWLKDFKQG
                     PTGKESTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNTIN
                     EMDKYYTQENYKDDAFAKGKTLHQTFLKNLESFEPVAESYHAAIQEINDKRQLAELKN
                     IEEREGKTFHYYSLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKEADKGG
                     MNFSFINSADQYQLETKKYVRRVRDKVPYSDWDKEHLQDANTSWMVEDSFPRALREYN
                     EMVDDYNSLR"
BASE COUNT         6469 a         6722 c         6505 g         6541 t
ORIGIN      
        1 aaataataat gaatatttag aacaatagtt ataaggcctg taaagcaggc cattttatca
       61 ttctaaatgg ttcttgattg attgcccaat tgcttttcct agttccacag gaacagcatt
      121 acctatcatt ttcccaatac ttcttataac aaacttgttt ttgtcttcta caaaaatata
      181 atccatcggg aaagtttgca ataaagcagc ctctctaaga gaaatcgctc tatgctgttc
      241 tggatgtcca aaacgcccat tcccgaaacc ataacaaaga gttgtaattg ttggacttgg
      301 cttatcccaa gacatgcgcc catatacact agcataccct ttaccgctgg atttcatatg
      361 acaagcggca accaactctt tcggccagtc cttccatgta ccaccaggaa cagagtgtat
      421 aattcttttt ttattaatcg gatttaattt actggctcta tgtaatatat catttggatc
      481 tgtttggcca tcttctaatg gaggaagatt tcctattgca tctctaactg ttttcaaaat
      541 aacatcgtca cgttttacta actcaatcgc ccccaatttt gaggccaaca atacatgacg
      601 ggaacggttt tgtgggattc catattcgac acaatcaatc ttagacgccc aaacataata
      661 gcccaacttt aacaatgaat tataaaaatc attataaact ttatgtttag ttacatcagg
      721 aacgttctcc attgttacga tttctggaga tacttcgcga attaaacgtt cgaactcata
      781 aagaagaggc cattttttat catcagcctt atcttttcct tgagtatatt ttgaaaaagg
      841 ttggcaaggt gcgcatcctg ccaatacttt tacagacgca tcaccataaa gcctcagcag
      901 ttcctctttt gttactttag cgatatcttg ctcaataaac ttagatttat tgtttctttc
      961 atagggaaac ctacattccc cctcaaggtc aatgcctgca acgacatcaa gccccgcaag
     1021 ctgcaaacca tgagttaatc cgccagcacc acaaaaaagg tctatcacct ttacagtcat
     1081 cactcactcc aaatctatac gatactcagt atacatccct ttctacaaaa tctcgaggcc
     1141 taacttataa actcatattt attgagttaa cgcttcactg agtttgactc taatactgtt
     1201 gagaatcccg accactcatc agcgagcggg taagtgaatt ttttcccgtc ataatttacg
     1261 gttgccccac gcgccagcac ctcaagctcc catcgctgcg gcctgatacc gttctgagca
     1321 aggtcaacgc ggatacgggt aatttgcatt cgttccgacc gggtcagtct ggccgatggc
     1381 gctatttcat gcggttttaa cgggcttccg tttctttgct gacggtttgg tcttctcagt
     1441 ccgtgtttta atgcacctct gagcgccctc acaacctccg ggtcattcca ttcgataaca
     1501 ccgtcatcta ccagattaag cactgctgcg gcgtgctcag aaggtgtggg agccggtaac
     1561 gaagtatcac caccagtgag ctttccacag ttattgacag gactccgagg cgcggcgatg
     1621 ccgcttttta aagtcaaagg ctcaacgacc ggcactttcg gaacaatgcg ccagtccgtc
     1681 gttctggtga tatgaatatg acgcgcgccg agatgcggcg cgtaaatgcc gaccactctc
     1741 tcgacttctt cctcgtactc gttaacttca tccgacggac tacgggcgac cctgacagtc
     1801 tgacaatcgc gcgggacatt tgccccaccc tgcgcgctga tatacagcgc aaaatcacca
     1861 ctgtctgcgg cggcgcgtgc agcctcgacg cgctcgtcaa actcatcagc aatgctgacg
     1921 ccgcgaggca atttgcgtag ttcacggtaa gcccccattg tcggcaggcc aaccgtttta
     1981 aattgcggaa tgcgccacgt tgacgcccat gcggtaacag ccgcggcagt atctttcagc
     2041 ggtctgccgc tatcgttatc gagctgccca tccagtgcat agccgtcgat attttttgag
     2101 atgtatttcg cgatataccc cgcagcaccg ccccgattaa gatgttttgc ctgaaaacgg
     2161 tttcgcgcag ctcctctttc gtcgccatcc tctttgagcg cgtagcgacg catgatttcg
     2221 ataatctggt tacgctggcg tggattacaa aaaagcatca tatgccagtg cggcgttccg
     2281 tcgtggtgtg gctcgacgac tcgcaaaccg tagacctgta aatcattatc cttgaatgcc
     2341 gtgcgcatca ggctccagat acggcagaga taacgctgcg catcctttgg attaaatgcc
     2401 tcatcgttcc agccgtgatt tagctggacg gttttacttt cgccttttcc aacctgacgt
     2461 gtcgggtgat actttgacgg cgcggtcagc gtgataaaca tccccacatc accttctgcg
     2521 gcggcgtaac gctcaatacc ggcaatggtg ttcatcagct ccatccggcg aatttcagga
     2581 ttagaaatac tgcccatcac cttactgata aggtcgatgc gctcgcctgt ttccctgttt
     2641 tcgagatcac acgatttaag aaattccaga tttgcctggc ggcgtgcacg cacatcacga
     2701 atggcatgtt tactggcata aggagaacgg tctttattga cctccccgac agcaatcagt
     2761 aacgcctcat gccagcgcat acgctggcct ttaagctgac taatccacca ctcatcgtta
     2821 aacagacggg caatggcaga atatgcctgc ctcgtggtca tctgtccttt acggtatttt
     2881 ttccagtaga gaggggaaat attgaaagca cgtgcagcgc cagcaacatg accatagagg
     2941 tgagcctgcg cctcatccgt aaacagcgat tctttttcgc catgcgcatc cacccaggca
     3001 tcgctgagtt cctcatacat catgaaaagc tgcgatgaga tacgggcggc aaactttttc
     3061 agctccttgt cattcattcc cggcaggcgc gcatagtggt cacgctcagc cagaaacagc
     3121 atcgacgcgt cggtgttcat ttcatggcgc tgattcacac gctcaatgcg cggccataaa
     3181 cgacgctgaa aagtggatgt gaggaaataa aacccgtgca ccgggctttt attgcgccgg
     3241 atgtagtcat agcgtgacgt aaacagcgag cgcaaaaagt aaggcaggcg gttaatcgtg
     3301 gataaaacac cttgcacctg acgcatctcg tcacgtgtaa ggggtctttc gcgcccgaca
     3361 gcctcgcgtg gcgcgttcca tgcataagca ccggtaaacg ccttaccggt gcctgcagca
     3421 aatgctgacg gagggacaaa acgcccggag gctttaacgg ccatatgagc caaaagcctc
     3481 tgaacaacgc ttgctgagtt gctcaacctg cgcgtttaaa tcagcaaaag actttgcgct
     3541 tccggtcaga atatcgtgat gcatcaggcc ggaaacgagc tggcttaatt tcgggtaata
     3601 accaaccacc gccagccatt cctgaccggc gtttttaccg ctttccgctc tctttttctc
     3661 gtggagaata aactggaagc tgtcactggt aacgacataa cgttcgccaa tttcaatacg
     3721 aatactcatg ccgttctccg gtaatgtttg ttttttgctt caaagactga ctgacaggaa
     3781 acacaacgcg tggctgacgg ataagccgca cgacgggcag caggaattgg cgcgtcacac
     3841 tcttcgcaaa ccagcgcaga agcaccgcaa tgttttaccc ttgccgcgtt aatctggcgc
     3901 tccagtaatt cagcctgttg ttcctgaata aaatctatgt tgtccggcat tatcagctcc
     3961 ttttatcgtt aagtttcctg gatacatcag cgcaataact ggcaagttct gtcgttaatt
     4021 ttgtcagttc atccactgag gaaatttgct tgtggaatac agcgcgttta acaagtaaat
     4081 tgaccacatc agacaggagg tttaattcat tctgataaat cgcgataaca gattcagtga
     4141 tgtcgcgttt ttctttatca agacaaagtt gaataagaga caagtcgcca tttttcataa
     4201 cggcgatttt taaggcgtta ttcagtaata caactgaatg agaacaggac atcaaagcac
     4261 ctccccgcga gacaatccga tattgtgaaa tttttccgac tcctgactga gcagctcgac
     4321 tatctccacg cgggataact ccgcctttgt gatatggcga atcatggcgt caagatgaga
     4381 agaaaagcgc gtcgcagcgt cggcctgtgc ttcggttctg gcctgttgca gcagtaatgc
     4441 gtatttaccg cactgatttt cagaaactgt atgcatgact ttctccaggc aaaaagaagc
     4501 cccgcacaat taagtgcgtt aaaaactctg gttaattatt taatgcaaat attgctctgg
     4561 ttttaccgac gtcagaattg tcggtgcata ctcaaacaga ctgaatagtt cacgtaatgc
     4621 acggaataaa gcatcacgcc agtaacatga ctcttcatta attcgccagt atggctgatt
     4681 gaattctttt tcagtcaatc cggcatgcat aaataaagta cgacgctgac tgactgttaa
     4741 aaaactaata tatgcatact cacttgcgcc aacctgacgg cgttttgaga atgccccacg
     4801 caattcatca attgcacaaa ccagccgttc acgttcgacg tcgttcattt cttcaaaacg
     4861 catcgttgcg tgacgttgtt ttaactgcgc atgaaagcaa actgttagcc gttcgcgctc
     4921 catcatctga ttataataat cacatgtatc ctgccaacga gggacggcaa gatgcttacc
     4981 aattatccgg cgcatagctg ctggctgttt ttcgacgaga ttgagcgtca tcactgtcat
     5041 ttccagaccc tccggctttt cagaaaggtc agagcctttt ttaacggact ctgttttttg
     5101 gtgcggataa tgattccctt gcgacccttc ccgtgggtga tggtgaagtc aatcgcccta
     5161 gggctttcgt tacgcaataa ctgagcaata caacgcggct cattcataat cacaacccca
     5221 tccacaaaag ccatgcatca cgctgttcaa ccggtcggtt ataaaacgcc tcacgtacag
     5281 cgcgattaaa ctcaggaatg aaaacccatt tttcaccggc acgagccttc ggtttgcaag
     5341 gatcacgcaa ttcaataatt ggtaatttat ttgccttcac catttcactg acggctgtct
     5401 ttggcttccc taataaatca gcaaatttat ccacatgaac cgcatcaagc ggatactgaa
     5461 tcacataatt ttcagcgtcc atatatggta ccctcatagg atccagccct ttctaaacca
     5521 ctcaaaaccg tttagacgct ggtttattct caaatcaatg gaacctatat aggttccagt
     5581 tttgagggaa tttagtccct atataggcac catgtcaaat gaaattaagc gaaaagatta
     5641 aggccttgcg tgaggctgaa gggctaagcc aatcaaaatt ctgtgaaatc atagagttac
     5701 cgctaagcac acttaaaaaa tatgaaggag gaaactttga acccggtggc acagctttgc
     5761 taaaaatcac tatgcatccc acattccaaa aatatgctct atggcttatg acagataaaa
     5821 ccgcgccgga cgcaggacaa atcgcaccgg ctctcgcgca cattgggcca gagtcaacag
     5881 agtccaacca ctccgcgaaa aggattggct aactctatat aaagattaca ttttcaccat
     5941 ttgctaccaa gatggtgaat acagcgccgg agggctttct tatggcaatt aagaagctcg
     6001 atgatggtcg ctatgaagtg gacattagac ctcgcggtcg cgacggaaaa cgcatccgca
     6061 ggaaattcga aagaaaagct gaagcactag catttgagcg atacacaatc gccaatgcca
     6121 gtcagaaaga atggggaggc cagcgagcag accgccggac tttgagtgag ttgctggaca
     6181 tctggtggaa atatcacggg caaaaccacg agcatggaac aaaagagttt aatcatctac
     6241 tcaaaaccat cagcggcata ggtgatatac cagtgagcag gatgagcaaa agggctttga
     6301 tggattatcg ttccatgcga ctacgtgatg gtatcagtgc cgcaacgata aaccgtgaca
     6361 tgtacagatt atccggcatg ttcacaaaat taattcaatt ggatgaattt tccgggcaac
     6421 acccaattca cggactgccg ccactggcgg aggccaaccc tgaaatgacg ttcctggaaa
     6481 aagcagaaat cgaaaaactg ttaaatgttt tggctggtga tgacttactt gtcgcgcttt
     6541 tatgtctgag cactggagga agatggacga aagttgccac gctaaaacca gcacagatta
     6601 caaattgcag ggttaccttc ctgaaaacca aaaacggtaa aaagcgaacc gtgccgattt
     6661 ctgaggaact ggagaaaaaa gttaaagagg aggccagcgc caaattattc aaagttgatt
     6721 atgagaaatt ttgcgggatt ttacgcagag tgaaacctga tataccaccc aatcaggcaa
     6781 cccacatcct gcggcataca ttcgcaagcc atttcatgat gaatgggggc aatataatcg
     6841 cactgcaaca gattctggga catgcgagca ttcagcagac gatggcctat gcgcaccttg
     6901 cgcctgacta tctgcaaaat gccgtcgctc tgaatccact aaaaggcgga gtgacgttat
     6961 aaatttcccc ctctgagtgt ccacatagtg tccacactca agaaattttg aaaccgctcc
     7021 aaacccttac agctttcgat aagtaactgt ttttactttt gattgcatgt aagtatttga
     7081 taaaaaaaac cccacatcat gtgggggaag acagggatgg tgtctatggc aaggaaaaca
     7141 gggtttacta ctgggaacgt gagttgctac tactcaatag cttcaacgat gaactttttt
     7201 gccattgcgt cacgtcacgc aactgctcca ttcgttgttg atgtttctcg tttaaaaccg
     7261 cttgctgctc cggcgttaac aggcgataca tttggttgcg gacttttgcc atctcaacct
     7321 gacgagcaat ttgctcattc gccatttttt ctgcctgtgc gcgcacagcg ttttcatcaa
     7381 aattttctgc ggtgacaagg cgatgcattg tctccagttc gctaacatta acaggaggct
     7441 gttcgtgccg ggcctgttgc ataagatctc gcatctgctg acgctgatgt tcggttaaac
     7501 ttatgccgtc gaacatatgg ctctgcgtac tgcgctgcgt aagttcttca cccggatgcc
     7561 agttatcgcc tgaaccgact tcagcagcgt ggcttaatga actgactgcc agcgttgagg
     7621 ccatgacggc agcggtaact atgcgcatca tttgctccca aaatctttct gtcgcgattc
     7681 aacgatagag agtttacgat tcaggctgca aacatgcgtc agggggtgta aaacaacgta
     7741 aagtcatgga ttagcgacgt ctgatgacgt aatttctgcc tcggaggtat ttaaacaatg
     7801 aataaaatcc tgttagttga tgatgaccga gagctgactt ccctattaaa ggagctgctc
     7861 gagatggaag gcttcaacgt gattgttgcc cacgatgggg aacaggcgct tgatcttctg
     7921 gacgacagca ttgatttact tttgcttgac gtaatgatgc cgaagaaaaa tggtatcgac
     7981 acattaaaag cacttcgcca gacacaccag acgcctgtca ttatgttgac ggcgcgcggc
     8041 agtgaacttg atcgcgttct cggccttgag ctgggcgcag atgactatct cccgaaaccg
     8101 tttaatgatc gtgagctggt ggcacgtatt cgcgcgatcc tgcgccgttc gcactggagc
     8161 gagcaacagc aaaacaacga caacggttca ccgacactgg aagttgatgc cttagtgctg
     8221 aatccaggcc gtcaggaagc cagcttcgac gggcaaacgc tggagttaac cggtactgag
     8281 tttaccctgc tctatttgct ggcacagcat ctgggtcagg tggtttcccg tgaacattta
     8341 agccaggaag tgttgggcaa acgcctgacg cctttcgacc gcgctattga tatgcacatt
     8401 tccaacctgc gtcgtaaact gccggatcgt aaagatggtc acccgtggtt taaaaccttg
     8461 cgtggtcgcg gctatctgat ggtttctgct tcatgatagg cagcttaacc gcgcgcatct
     8521 tcgccatctt ctggctgacg ctggcgctgg tgttgatgtt ggttttgatg ttacccaagc
     8581 tcgattcacg ccagatgacc gagcttctgg atagcgaaca gcgtcagggg ctgatgattg
     8641 agcagcatgt cgaagcggag ctggcgaacg atccgcccaa cgatttaatg tggtggcggc
     8701 gtctgttccg ggcgattgat aagtgggcac cgccaggaca gcgtttgtta ttggtgacca
     8761 ccgaaggccg cgtgatcggc gctgaacgca gcgaaatgca gatcattcgt aactttattg
     8821 gtcaggccga taacgccgat catccgcaga agaaaaagta tggccgcgtg gaactggtcg
     8881 gtccgttctc cgtgcgtgat ggcgaagata attaccaact ttatctgatt cgtccggcca
     8941 gcagttctca atccgatttc attaacttac tgtttgaccg cccgctatta ctgctgattg
     9001 tcaccatgtt ggtcagtacg ccgctgctgt tgtggttggc ctggagtctg gcaaaaccgg
     9061 cgcgtaagct gaaaaacgct gccgatgaag ttgcccaggg aaacttacgc cagcacccgg
     9121 aactggaagc ggggccacag gaattccttg ccgcaggtgc cagttttaac cagatggtca
     9181 ccgcgctgga gcgcatgatg acctctcagc agcgtctgct ttctgatatc tctcacgagc
     9241 tgcgcacccc gctgacgcgt ctgcaactgg gtacggcgtt actgcgccgt cgtagcggtg
     9301 aaagcaagga actcgagcgt attgaaaccg aagcgcaacg tctggacagc atgatcaacg
     9361 atctgttggt gatgtcacgt aatcagcaaa aaaacgcgct ggttagcgaa accatcaaag
     9421 ccaaccagtt gtggagtgaa gtgctggata acgcggcgtt cgaagccgag caaatgggca
     9481 agtcgttgac agttaacttc ccgcctgggc cgtggccgct gtacggcaat ccgaacgccc
     9541 tggaaagtgc gctggaaaac attgttcgta atgctctgcg ttattcccat acgaagattg
     9601 aagtgggctt tgcggtagat aaagacggta tcaccattac ggtggacgac gatggtcctg
     9661 gcgttagccc ggaagatcgc gaacagattt tccgtccgtt ctatcggacc gatgaagcac
     9721 gcgatcgtga atctggcggt acaggattgg ggctggcgat tgttgaaacc gccattcagc
     9781 agcatcgtgg ctgggtgaag gcagaagaca gcccgctggg cggtttacgg ctggtgattt
     9841 ggttgccgct gtataagcgg agttaaactc cgcatttgta ggcaggataa ggcgtttacg
     9901 ccgcatccgg catttgagca ggatgcctga tgcgacgctg atagcgtctt atcaggccta
     9961 cactccgggg gttattttcc ccacaaccgg cgggaaaaat cttcgatctt gccgctcagt
    10021 cggcgctttt gcatcgtccg cgtccagctt ttcgataacc cggcggcgga gagtaaacgg
    10081 tgatactgat cgtcatcaaa cggcatatgc catgcgatgg cggcagcctc ctgcacggtc
    10141 acatcgctga cacgggaaac caactccagc ggcgcgtccg cagacacttt ccctggcgct
    10201 atcacgctat acaaccaccc caccttacct gtgttttgca tcaactgcgc tatatcgctg
    10261 atatcaaaat ggtaattgag tttatagcaa ggcgagcgcg gctggctgac ctgaattaat
    10321 gcctctcccc agcggaaaat atcgcccata tgaacattac tttccgtgag accgtcggtt
    10381 gagagatttt cgccaaacgc aggcgcaaca aacaactccg cctgttccgg aaattcccgc
    10441 acccagtaga gatagtgctc acgaggataa tgacacagcg ctctgtctgg cccgccgtga
    10501 acctttttct ccgcctgctc gtcaccttcc agccccagct ccgtcagcat caactcacca
    10561 tcaacctgga ttttggcaat tgcgctgggt ttgccttcgg gataagcctg aatcttgcct
    10621 gtgtatacat caaccggata tcgcatctgt tccctctcca cgagaaattt ccccattaga
    10681 ccatatttat tttatgtcag gcgtgctgtt ttcgcggtaa gtatccaggc aggaactcaa
    10741 tagcaaaccg gacgaacgtt agttcatccg gctgtgggga ttaaggaagc aggtttattt
    10801 caccgtacct aaaatttcga ttttcgctgc tctggctttg actttacgtg accagataga
    10861 ggtaagaatt ggcaccagaa tcgaagtcac aatgaccgcc gtcgctacca gcgaagttgc
    10921 tgccggagcc atcggtttaa acgcaggcac catttctgca atcagcacag gtgtcgctac
    10981 cgcggcccct gcggaactgg aagcggcaat tccggcagtg ccatcgccac cgccaatcaa
    11041 tttatcggcg ataatcaacg gaataccggt cacgataatt actgccacac ccaacaggat
    11101 ccccagcaaa ccagtctggg caatcacagt caaatcaatg gtattgccca gcgcgaaggc
    11161 aaagaatgga atcagcgttt gcactgcttt gctgaaaaat tctcgtaatt cagggtcaag
    11221 attccccagg gcaaagccca ccaggaacgg taatactgcg ccgacgaaaa catgcggttc
    11281 aaacgaggca atcccggcag tgcccagaat aatcatcgtc atgagcggcc cggactccaa
    11341 cgacatcaac acaaatgccc cagcttcttc tttcgtaccg tactgctgca tgatggaagc
    11401 gtaaagtccg ccgttggtca tatccatcgc cgccaccagc gccagcgttg aaagtccggc
    11461 aaagaatcca acttcaacac catgttccgg aataatgcgc gaggcaattg ccgcaaccac
    11521 ccacgcgacg gcaattttag ttaccaccag cgtaccggat ttacgcagta ccgttcccgt
    11581 cgcgcttaat tttattgacg cccccatgca aaaaaaccac accgccagaa tgggcaccgt
    11641 accggtaatc atcccgttgg tgaatgatcc aaaatatttc cccgcccccg gcgagaaggt
    11701 gtggcacagt gcgccaagga atagcgggac gagcatcatc ccccccggga ttttctcaat
    11761 cgagcgtttt atctgcattt ccatcacctg tcggcatatt tttaagggct gatgcgtaat
    11821 tcctcaatgg agcatcagtg ttgatggaaa cgaagataga cattcattta atcaataaaa
    11881 gtgatcaaaa ccacatcaat tgaaacgctg ttttattttt atcggatcat tgtttttatt
    11941 ttgaaattga tcataaaaaa accaccgccg ttggcgatgg ttcatttttt aagctgatat
    12001 gcggcctata cgcctcattg cagcaggcgg caaatgatta ttttttcgcc gcaaaacgtg
    12061 ccgctgcttc gtcccagttc accacgttcc agaactcttt aatgtagtcc ggacggcggt
    12121 tctggaattt caggtagtaa gcatgttccc acacatccag gcccagaatc gggaagccgg
    12181 aagcgccaga aatagcttca cccatcagcg gagaatcctg gttagcagta gaaaccaccg
    12241 ccagtttatc gcctttcagc accagccatg cccagccgga accaaagcgg gaagctgccg
    12301 ctttttcaaa ttctgctttg aagttatcaa cggagccgaa gtcacgttcg atagccgctt
    12361 tcaggtcacc ctgcagggtg gtgccttttt tcagaccttt ccagaacagg ctgtggttag
    12421 cgtgaccgcc agcgttgttg cgcagtacgg ttttcttgtc tgctggcagc tggtccagtt
    12481 tggtgatcag ctcttcaacc ggcaggttgg caaattctgg caggctttcc agcgccgcgt
    12541 tggcgttgtt tacgtaggtc tgatggtgtt tggtgtggtg gatttccatg gtctgcttat
    12601 cgaagtgcgg ttccagggca tcgtaagcat acggcaggga tggcagggta tagctcatat
    12661 tcatctccag tattgtcggg cggccgattg ttaatgccgc gtaagcagtt ggttcattat
    12721 agttaattaa atgatattga aaatgattat caatgccgta cttttcgtaa gggtatggtt
    12781 ttgcaggaaa atgcccgaga tgtgaagcaa atcacccact taatgccgtg attgccagta
    12841 aatcgacaac ggcggcaaca ggcgaaaggt taatcgacag cacgattttt acactcatct
    12901 cgtcggagat gtgaagcgac gaaaaatgat gaggataaga agatgagtaa cgcgattacg
    12961 atggggatat tttggcattt gatcggcgcg gccagtgcag cctgttttta cgctccgttc
    13021 aaaaaagtaa aaaaatggtc atgggaaacc atgtggtcag tcggtgggat tgtttcgtgg
    13081 attattctgc cgtggaccat cagcgccctg ttactaccgg atttctgggc gtattacagc
    13141 tcgtttagtc tctctacgct gctgcctgtt tttctgttcg gcgctatgtg ggggatcggt
    13201 aatatcaact atggcctgac catgcgttat ctcggcatgt cgatgggaat tggcatcgcc
    13261 attggcatta cgttgattgt cggtacgctg atgacgccaa ttatcaacgg caatttcgat
    13321 gtgctgatta acaccgaagg cggacgcatg acgttgctcg gcgttctggt ggcgctgatt
    13381 ggcgtaggga ttgtaactcg agccgggcag ttgaaagagc gcaagatggg cattaaagcc
    13441 gaagagttca atctgaaaaa agggctggtg ctggcggtga tgtgcggcat tttctctgcc
    13501 gggatgtcct ttgcgatgaa cgccgcaaaa ccgatgcatg aagccgctgc cgcacttggc
    13561 gtcgatccac tgtatgtcgc tctgccaagc tatgttgtca tcatgggcgg cggcgcgatc
    13621 attaacctcg gtttctgttt tattcgtctg gcaaaagtga aggatttgtc gctaaaagcc
    13681 gacttctcgc tggcaaaacc gctgatcatt cacaatgtgt tactctcggc actgggcggg
    13741 ttgatgtggt atctgcaatt ctttttctat gcctggggcc acgcccgcat tccggcgcag
    13801 tatgactaca tcagttggat gctgcatatg agtttctatg tattgtgcgg cggtatcgtc
    13861 gggctggtgc tgaaagagtg gaacaataca ggacgccgtc cggtaacggt gttgagcctc
    13921 ggttgtgtgg tgattattgt cgccgctaac atcgtcggca tcggcatggc gaattaatct
    13981 ttctgcgaat tgagatgacg ccactggctg ggcgtcatcc cggtttcccg ggtaaacacc
    14041 accgaaaaat agttactatc ttcaaagcca cattcggtcg aaatatcact gattaacagg
    14101 cggctatgct ggagaagata ttgcgcatga cacactctga cctgtcgcag atattgattg
    14161 atggtcattc cagtctgctg gcgaaattgc tgacgcaaaa cgcgctcact gcacgatgcc
    14221 tcatcacaaa atttatccag cgcaaaggga cttttcaggc tagccgccaa ccgggtaatc
    14281 agcttatcca gcaacgtttc gctggatgtt ggcggcaacg aatcactggt gtaacgatgg
    14341 cgattcagca acatcaccaa ctgcccgaac agcaactcag ccatttcgtt agcaaacgac
    14401 acatgctgac tactttcatg ctcaagctga ccgataacct gccgcgcctg cgccatcccc
    14461 acgctaccta agcgccagtg tggttgccct gcgctggcgc taaatcccgg aatcgctccc
    14521 tgccagtcaa gattcagctt cagccgctcc gggcaataaa taatattctg caaaaccaga
    14581 tcgttaacgg aagcgtagga gtgtttatcg tcagcatgaa tataaaagag atcgccacgg
    14641 gtaatgcgat aaggacgatc gttgagtaca tgcagaccat taccgcgcca gacaatcacc
    14701 agctcacaaa aatcatgtgt atgttcggca aagacatctt gcggataacg gtcagccaca
    14761 gcgactgcct gctggtcgct ggcaaaaaaa tcatctttga gaagttttaa ctgatgcgcc
    14821 accgtggcta cctcggccag agaacgaagt tgattattcg caatatggcg tacaaatacg
    14881 ttgagaagat tcgcgttatt gcagaaagcc atcccgtccc tggcgaatat cacgcggtga
    14941 ccagttaaac tctcggcgaa aaagcgtcga aaagtggtta ctgtcgctga atccacagcg
    15001 ataggcgatg tcagtaacgc tggcctcgct gtggcgtagc agatgtcggg ctttcatcag
    15061 tcgcaggcgg ttcaggtatc gctgaggcgt cagtcccgtt tgctgcttaa gctgccgatg
    15121 tagcgtacgc agtgaaagag aaaattgatc cgccacggca tcccaattca cctcatcggc
    15181 aaaatggtcc tccagccagg ccagaagcaa gttgagacgt gatgcgctgt tttccaggtt
    15241 ctcctgcaaa ctgcttttac gcagcaagag cagtaattgc ataaacaaga tctcgcgact
    15301 ggcggtcgag ggtaaatcat tttccccttc ctgctgttcc atctgtgcaa ccagctgtcg
    15361 cacctgctgc aatacgctgt ggttaacgcg ccagtgagac ggatactgcc catccagctc
    15421 ttgtggcagc aactgattca gcccggcgag aaactgaaat cgatccggcg agcgatacag
    15481 cacattggtc agacacagat tatcggtatg ttcatacaga tgccgatcat gagcgcgtac
    15541 gaaacagacc gtgccaccgg tgatggtata gggctgccca ttaaacacat gaatacccgt
    15601 gccatgttcg acaatcacaa tttcatgaaa atcatgatga tgttcaggaa aatccgcctg
    15661 cgggagccgg ggttctatcg ccacggacgc gttaccagac ggaaaaaaat ccacactatg
    15721 taatacggtc atactggcct cctgatgtcg tcaacacggc gaaatagtaa tcacgaggtc
    15781 aggttcttac cttaaatttt cgacggaaaa ccacgtaaaa aacgtcgatt tttcaagata
    15841 cagcgtgaat tttcaggaaa tgcggtgagc atcacatcac cacaatgaag caaattgtga
    15901 acatcatcac gttcatcttt ccctggttgc caatggccca ttttcctgtc agtaacgaga
    15961 aggtcacgaa ttcaggcgct ttttagactg gtcgtaatga aattcagcag gatcacatta
    16021 tgacctttcg caattgtgtc gccgtcgatc tcggcgcatc cagtgggcgc gtgatgctgg
    16081 cgcgttacga gcgtgaatgc cgcagcctga cgctgcgcga aatccatcgt tttaacaatg
    16141 gcctgcatag ccagaacggt tatgtcacct ggaatgtgga tagcctggaa agtgccattc
    16201 gccttggatt aaacaaggtg tgcgaggaag ggattcgtat cgatagcatt gggattgata
    16261 cctggggcgt ggactttgtg ctgctcgacc aacagggtca gcgtgtgggc ctgcccgttg
    16321 cttatcgcga tagccgctcc aatggcctaa tggcgcaggc acagcaacaa ctcggcaaac
    16381 gcgatattta tcaacgtagc ggcatccagt ttctgccctt caatacgctt tatcaactgc
    16441 gtgcgctgac ggagcaacaa cctgaactta ttccacacat tgctcacgct ctgctgatgc
    16501 cggattactt cagctatcgc ctgaccggca agatgaactg ggagtacacc aacgccacga
    16561 ccacgcaact ggtcaatatc aatagcgacg actgggacga gtcgctactg gcgtggagcg
    16621 gggctaacaa agcctggttt ggtcgcccga cgcatccggg taatgtcatt gggcactgga
    16681 tttgcccgca gggtaatgag attccggtgg tcgccgttgc cagccatgat accgccagcg
    16741 cggttatcgc ctcgccgtta aacggttcac gcgctgctta tctctcttct ggcacctggt
    16801 cattgatggg cttcgaaagc cagacgccat ttaccaatga cacggcgctg gcagccaaca
    16861 taaccaatga aggcggggcg gaaggtcgct atcgggtgct gaaaaatatt atgggcttat
    16921 ggctgcttca gcgagtgctt caggagcggc aaatcaacga tctcccggcg cttatcgccg
    16981 cgacacaggc acttccggcc tgccgcttca tcatcaatcc caatgacgat cgctttatta
    17041 atcctgaggc gatgtgcagc gaaattcagg ctgcgtgtcg ggaaatggcg caaccgctcc
    17101 cggaaagtga tgctgaactg gcgcgctgca ttttcgacag tctggcgctg ctgtatgccg
    17161 atgtgttgca tgagctggcg cagctacgcg gtgaagattt ctcgcaactg catattgtcg
    17221 gcggcggctg ccagaacacg ctgctcaacc agctatgtgc cgatgcctgc ggtattcggg
    17281 tgatcgccgg gcctgttgaa gcctcgacgc tcggcaatat cggcatccag ttaatgacgc
    17341 tggatgaact caacaatgtg gatgatttcc gtcaggtcgt cagcaccacc gcgaatctga
    17401 ccacctttac ccctaatcct gacagtgaaa ttgcccacta tgtggcgcag cttcactcga
    17461 cacgacagac aaaggagctt tgcgcatgac cactcaactg gaacaggcct gggagctggc
    17521 gaaacagcgt ttcgcggcgg tggggattga tgtcgaggag gcgctgcgcc aacttgatcg
    17581 tttacccgtt tcaatgcact gctggcaggg cgatgatgtt tccggttttg aaaacccgga
    17641 aggttcgctg accggtggga ttcaggctac tggtaattat ccgggcaaag cgcgtaatgc
    17701 cagtgagcta cgagccgatc tggaacaggc tatgcggctg attccggggc cgaaacggct
    17761 taatttacat gccatctatc tggaatcaga cacgccagtc tcgcgcgacc agatcaaacc
    17821 tgagcacttc aaaaactggg ttgaatgggc gaaagccaat cagctcggtc tggattttaa
    17881 cccctcctgt ttctcgcatc cgctaagcgc cgatggcttt acgctctcgc atcccgacga
    17941 cagcattcgc cagttctgga ttgatcactg caaagccagc cgtcgcgttt cggcctattt
    18001 tggcgagcaa ctcggcacac catcggtgat gaacatctgg atcccggatg gtatgaaaga
    18061 tatcaccgtt gaccgtctcg ccccgcgtca gcgtctgctg gcagcactgg atgaggtgat
    18121 cagcgagaag ctggatccgg cgcaccatat cgacgccgtt gagagcaaat tgtttggcat
    18181 tggcgcagag agctacacgg ttggctccaa tgagttttac atggggtatg ccaccagccg
    18241 ccagactgcg ctgtgcctgg acgccgggca tttccacccg actgaagtga tttccgacaa
    18301 gatttccgcc gccatgctgt atgtaccgca gttgctgctg cacgtcagcc gtccggttcg
    18361 ctgggacagc gatcacgtgg tgctgctgga tgatgaaacc caggcgattg ccagtgagat
    18421 tgttcgtcac gatctgttcg accgggtgca tatcggcctc gacttctttg atgcctctat
    18481 caaccgcatt gccgcgtggg tcattggtac acgcaatatg aaaaaagccc tgctgcgtgc
    18541 gttgctggaa cctaccgctg agctgcgcaa gctggaagcg gcgggcgatt acactgcgcg
    18601 tctggcgcta ctggaagaac agaaatcgtt gccgtggcag gcggtctggg aaatgtattg
    18661 ccaacgtcac gatacgccag caggtagcga atggctggag agcgtgcggg cttatgagaa
    18721 agcgattttg agccagcgtg ggtaaacact gccggatgcg gcgcgagcgc cttatccggc
    18781 ctacaggtcg aatgaccatg cgtgctgcca ccctcacccc ggccctctcc ctgaaagggc
    18841 gagggagaaa aacatagcct cgtgcggcaa cggagctgaa caccgctccg gacagtcccc
    18901 tcgcccctac ggggagaggg ttagggtgag gggaacaagc cgcaccatgt ctaaacaagg
    18961 caacctaatt tacaggaaca cagaacatgc aaaacattac ccagtcctgg tttgtccagg
    19021 gaatgatcaa agccaccacc gatgcctggc tgaaaggctg ggacgagcgc aatggtggta
    19081 acctgacgct gcgcctggat gacgccgata tcgcgcctta tcacggcaac tttcatgcac
    19141 aaccgcgcta tattcccctc agccaaccca tgcctttact ggcaaatacg ccgtttattg
    19201 tcaccggctc cggcaaattc ttccgtaacg tccagcttga tcctgcagct aacttaggcg
    19261 tcgtaaaagt cgacagcgac ggcgcgggct accatattct ctgggggtta accaacgaag
    19321 ccgttccaac ttccgaactt ccggctcact tcctttccca ctgcgagcgc attaaagcca
    19381 ccaacggtaa agatcgggtg atcatgcact gccacgccac caacctgatc gccctcacct
    19441 atgtacttga aaacgacacc gcggtcttta ctcgccaact gtgggaaggc agcaccgagt
    19501 gtctggtggt attcccggat ggcgttggca ttctgccgtg gatggtgccc ggcacggacg
    19561 aaatcggcca ggcgaccgca caagagatgc aaaaacattc gctggtgttg tggcccttcc
    19621 acggcgtctt cggcagcggg tcgacgttgg atgaaacatt cggtttaatc gacaccgcag
    19681 aaaaatcagc acaagtatta gtgaaggttt attcgatggg cggcatgaaa cagaccatca
    19741 gccgtgaaga gttgatagcg ctcggcaagc gcttcggcgt tacaccacta gctagtgcgc
    19801 tggcgctgta aggagcaaac atgatccgca aagcctttgt catgcaggtt aaccccgacg
    19861 cccacgaaga gtatcagcgt cggcataacc ccatctggcc ggaactggaa gcagtgttga
    19921 aatctcacgg tgcgcataac tacgccattt acctcgacaa agcgcgtaat ctgctgtttg
    19981 ccacggtaga gattgaatct gaagaacggt ggaatgcggt agccagcact gatgtttgcc
    20041 aacgttggtg gaaatatatg accgatgtta tgcccgctaa cccggataac agcccggtga
    20101 gtagcgagct gcaagaagtg ttttacctgc cgtaattgcc caatgtgcca gagatgcctc
    20161 cggcacaata ttttaaattt cgatttgaaa aattaaacat attacatctc ccggtaagtt
    20221 atatttccct gatacattgt gagtaaatca caaaaataat gaataaccca ttaatgattc
    20281 atgtggttta tttaaataac cattatgtgc attactccgc aaatctgacc tttcactctg
    20341 tctcaaattg tcattgttta ccgacatatc gcctctcatc acttaatcgc tccaccatta
    20401 caattgatta aaatatattt tatggagtcc gattaatggc agctcttact gcaagctgta
    20461 ttgacctgaa tattcagggc aatggcgctt attccgttct gaagcagttg gcgacaatag
    20521 cgttacaaaa cggttttatc accgactcac accagttcct gcaaaccctg ctgctgcgcg
    20581 aaaaaatgca ctctactgga tttggttccg gtgtcgccgt gccgcacggt aaaagcgcct
    20641 gcgttaaaca accgtttgta ttattcgccc gcaaagcgca ggctattgac tggaaagcca
    20701 gcgatggcga agacgtcaat tgctggatct gcctcggcgt gccgcaaagc ggcgaagagg
    20761 atcaggtcaa aatcatcggc acactgtgtc gcaaaattat ccaccaggat tttattcatc
    20821 aactgcaaca gggcgacgcc gaccaggtgc ttgccttgtt aaatcaaacc ctcagctcat
    20881 aaggaagtgg cgatggagtc atccttatgt attgtcgcga tcaccaactg ccccgccggg
    20941 atcgctcaca cctacatggt ggcggaagcc ctggaacaga aagcccgttc tctcggccat
    21001 accataaaag tggaaactca agggtccagt ggcgttgaaa accgcttatc cagcgaagag
    21061 attgccgctg ccgattacgt cattctcgct accgggcgtg gcctgagcgg tgatgatcgc
    21121 gcgcggtttg ccgggaagaa agtttatgag attgccatct cccaggcgtt gaaaaatatc
    21181 gaccagattt tcagcgaatt accgacaaac tcgcagcttt ttgccgcaga tagcggcgtg
    21241 aagcttggca aacaggaagt gcaaagcggc agcgtgatga gccacctgat ggcgggcgtc
    21301 tctgcggcgc tgccgtttgt catcggcggt ggtatcctgg tggcgcttgc caacatgctg
    21361 gtgcagttcg gtttgccgta taccgatatg tcgaaaggcg cgccgtcgtt tacctgggta
    21421 gttgaatcca tcggttacct tggcttcacc tttatgatcc ccatcatggg cgcttatatt
    21481 gcctcgtcga ttgccgataa accggctttc gctccggcgt ttctggtttg ctatctggcg
    21541 aacgacaaag cgctgctcgg cacccagtcg ggtgcgggat tcctcggcgc ggtggtgctg
    21601 gggctggcga ttggctattt cgtcttctgg ttccgcaaag tgcgccttgg caaagcatta
    21661 caaccgctgc tcggttcaat gctgatcccg ttcgttactc tgctggtttt cggcgtgctc
    21721 acttactacg ttatcgggcc ggtgatgtcc gacctcatgg gcgggctgct ccacttcctg
    21781 aataccattc ctccatcaat gaagtttgcg gcagcgtttc tggtcggtgc gatgctggcg
    21841 ttcgatatgg gcggccccat caataaaacc gcctggttct tctgcttctc actgctggaa
    21901 aaacacattt atgactggta cgccatcgtc ggcgttgtcg cgttgatgcc gcccgttgcg
    21961 gcgggtcttg cgacgttcat tgcgcccaaa ctgtttaccc gacaggaaaa agaggctgcc
    22021 agtagtgcca ttgtggtcgg tgccacagtc gcaaccgaac cggctatacc ttacgctctg
    22081 gcagctccgt tgccgatgat taccgccaat acgctggcgg gtggcattac cggcgtgctg
    22141 gtgatcgctt tcggaatcaa acgtctggca cctggtttgg gtatctttga cccgctgatc
    22201 ggcctgatgt cgccggtggg ttcgttttat ctggtgctag cgattggcct cgcgctgaac
    22261 atttcattca ttatcgtcct gaaaggattg tggttacgcc gcaaagcgaa agccgcgcaa
    22321 caggagcttg tccatgaaca ttgagttact gcaacagttg tgcgaagcca gcgccgtcag
    22381 cggcgatgaa caggaagttc gcgacattct gataaacacg ctggaacctt gcgttaatga
    22441 gatcaccttt gatggtctgg gcagctttgt tgcccgtaag gggaataaag gtccaaaagt
    22501 tgccgttgtc gggcatatgg atgaagtcgg ctttatggtc acccacatcg acgagagcgg
    22561 ttttctgcgc tttaccacca ttggcggctg gtggaatcag tcaatgctca accaccgggt
    22621 aaccatacgc acacacaagg gatttaaaat ccctggtgtg attggttccg tcgcacctca
    22681 tgcgttaacg gaaaagcaaa agcaacagcc gctgtcattt gatgagatgt tcattgatat
    22741 tggcgcgaac agtcgcgatg aggtggaaaa acgcggtgtg gcgatgggcg attttattag
    22801 cccggaagcc aattttgcct gctggggcga agataaagtg gtcggcaagg cgctggataa
    22861 ccgcatcggc tgcgcaatga tggctgaact attgcagacg gtgaataatc ccgaaattac
    22921 gctgtatggc gtcggcagcg tggaagaaga agttgggcta cgcggggcac aaacctcggc
    22981 ggaacacatt aaaccggatg tggtgattgt gctggatacc gctgtcgcag gtgatgttcc
    23041 gggcattgat aacattaaat acccgctgaa actgggccag gggccggggc tgatgctgtt
    23101 tgacaagcgc tacttcccca accagaaact ggtggcggcg ttaaaaagct gtgccgcaca
    23161 taacgattta ccgctgcaat tttccaccat gaaaaccggt gcgacggatg gcgggcgcta
    23221 caacgtgatg ggcggcgggc gtccggttgt tgcgctgtgc ctgccaaccc gctatctgca
    23281 cgctaacagc ggcatgattt caaaagccga ttacgatgct ctgctcacgt tgatacggga
    23341 ttttctgacc accttaaccg cggagaaagt caacgcgttt agccagttcc gtcaggtgga
    23401 ttaatgctaa acgaacgcca gttaaagatt gtcgatctgc tggagcaaca gccgcgcacg
    23461 cctggtgagc tggcgcaaca gactggcgtt tcaggcagga ccatcctgcg tgatattgac
    23521 tatctcaact ttacccttaa cggcaaagcc cgcattttcg ccagtggcag tgcgggctat
    23581 cagctggaaa tcttcgagcg ccgcagcttt tttcagttgc tgcaaaagca cgataacgac
    23641 gatcggctgc tggcgctgtt attactgaat acctttactc cccgtgcgca actcgcctcg
    23701 gcgcttaatt tgccagaaac ctgggtagca gagcgtctgc cccggttaaa acagcgttat
    23761 gaacgcactt gttgcctggc cagtcgcccc ggtttgggcc atttcattga tgagacagaa
    23821 gagaaacgcg ttatcttgct ggcgaacttg ctgcgcaaag atccgttttt aattccgctg
    23881 gcgggcgtaa cacgagacaa ttttcagcat ttatccacgg cctgcgacaa ccaacaccgc
    23941 tggccgctca tgcagggtga ttatctctcc agcctgattc tggcgattta cgccctgcgt
    24001 aatcaactga ccgatgagtg gccgcaatat cccggtaacg agataaaaca aatcgttgaa
    24061 cagagcggca tgtttcttgg tgataacgct gtaagaaccc tgacaggttt gatagagaaa
    24121 cagcatcagc aagcgcaggt aatttcagcc gatcatgtgc tggggttgct gcaaagggtg
    24181 ccaggcatcg cgtcattgaa tattattgat acgcagctgg ttgagaatat taccgggcat
    24241 ttattacgtt gccttgccgc accggtgtgg attgccgagc accgccaaag cagcatgaat
    24301 aacctgaaag ccgcctggcc tgcggccttt gatatgagtc tgcactttat tacgctactg
    24361 cgtgaacagc tcgatattcc ccttttcgat agcgatctga tcggtttgta ttttgcctgt
    24421 gcgctggaac ggcatcaaaa cgaacgccag ccgatcattt tgctctcgga ccagaacgcg
    24481 attgccacta ttaatcagct cgccattgag cgcgatgttt tacattgtcg ggtgattatt
    24541 gcccgtagct taagcgagct tattgccatt cgcgaagaga ttgagccgtt attgatcatt
    24601 aacaacagcc attatttact ggatgacgcg gtaaataatt acatcaccgt aaaaaatatc
    24661 attacggctg ccgatatcga acaaataaaa cactttctgg cgacggcatt tattcgccaa
    24721 cagccggaac gctttttctc tgcccccgga agttttcatt attcgaatgt acgcggtgaa
    24781 agctggcaac atattacccg gcaaatttgt gcgcaattag tggcacaaca ccatattacc
    24841 gccgatgaag cgcaacggat catcgcccgc gaaggcgaag gtgaaaacct gattgttaat
    24901 cgcctcgcca tcccacattg ctggagcgaa caggagcgac gttttcgtgg attttttatt
    24961 accctcgccc aaccagttga ggtgaataac gaagtcatta accatgtctt gatcgcctgc
    25021 gccgccgccg atgcgcgtca tgagctaaaa atatttagct atctggcaag cgtattgtgt
    25081 cagcatccgg cagaagttat tgccgggtta acaggatatg aggcatttat ggagttactt
    25141 cacaaggggt gaactgtgtc ggatgcgccg catccgacac tgcatttatc atcaaaaacg
    25201 tttaacgcag gctattatag tcatcaacca tttcgttgta ctcgcgtaac gctcgtggaa
    25261 atgagtcttc gaccatccag cttgtgtttg catcctgaag atgctcttta tcccagtcag
    25321 agtacgggac tttatctctg acgcggcgaa catatttttt agtctcgagt tgatactggt
    25381 ctgccgaatt aatgaacgag aaattcatac cgcctttatc cgcttctttg gcctgcgcta
    25441 ccagcgtttc cagttcagag actttcttca ttgccgcatc aacatcaaac ttctcttgcg
    25501 aaatcaggtt attaatttgt ttcgctgaaa tcatgactgc cagagagtag tagtggaatg
    25561 ttttcccttc tctttcttca atatttttca gttccgcaag ctggcgctta tcattaattt
    25621 cctgaatcgc cgcatgataa gattctgcta caggttcaaa ggattccaga ttttttaaga
    25681 atgtctggtg cagcgttttc cctttcgcaa acgcatcgtc tttataattt tcctgggtgt
    25741 aatatttatc catttcgtta atggtattac ccaacgccac ggcagcatca atataactca
    25801 ccgccacgcc atcaataggt tgcagcgcag gcgttaacgc cacagcactt tttacgcctt
    25861 tttcacactc agcgaggttg gattcactaa tgccgtagat gccataaaca gtactctctt
    25921 taccggtagg cccctgttta aaatctttca gccagtcagc ataacgcgcc agactgttct
    25981 gtacagggat ctgtaacttg ttgtagcact tgatatagac atccattttt tcgtcgaaaa
    26041 ccgcttgttc atcgaccacc ggttgcgcag gtgtttcagg tttagcttct gcttttgcaa
    26101 cgggagcttc aggagctggt gcggcaggtt gactattggc aggcgggggc gtttctgttt
    26161 cggctttttt atcatcacaa cccgtcagac cgagggccat gatggcgact ataatagcgg
    26221 aagataataa attcctt
//
LOCUS       DAZPDU010000043        25637 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZPDU010000043 DAZPDU010000000
VERSION     DAZPDU010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25637
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.043"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(65..796)
                     /gene="rlmB"
                     /locus_tag="ACHURS_001643"
     CDS             complement(65..796)
                     /gene="rlmB"
                     /locus_tag="ACHURS_001643"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFR1237408.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(976..3417)
                     /gene="rnr"
                     /locus_tag="ACHURS_001644"
     CDS             complement(976..3417)
                     /gene="rnr"
                     /locus_tag="ACHURS_001644"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFR1237409.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3456..3881)
                     /gene="nsrR"
                     /locus_tag="ACHURS_001645"
     CDS             complement(3456..3881)
                     /gene="nsrR"
                     /locus_tag="ACHURS_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFR1237410.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4086..5384)
                     /gene="purA"
                     /locus_tag="ACHURS_001646"
     CDS             complement(4086..5384)
                     /gene="purA"
                     /locus_tag="ACHURS_001646"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFR1237411.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVATTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5488..5685)
                     /locus_tag="ACHURS_001647"
     CDS             complement(5488..5685)
                     /locus_tag="ACHURS_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFR1237412.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5767..6771)
                     /gene="hflC"
                     /locus_tag="ACHURS_001648"
     CDS             complement(5767..6771)
                     /gene="hflC"
                     /locus_tag="ACHURS_001648"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFR1237413.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6774..8033)
                     /gene="hflK"
                     /locus_tag="ACHURS_001649"
     CDS             complement(6774..8033)
                     /gene="hflK"
                     /locus_tag="ACHURS_001649"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFR1237414.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8119..9399)
                     /gene="hflX"
                     /locus_tag="ACHURS_001650"
     CDS             complement(8119..9399)
                     /gene="hflX"
                     /locus_tag="ACHURS_001650"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFR1237415.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9476..9784)
                     /gene="hfq"
                     /locus_tag="ACHURS_001651"
     CDS             complement(9476..9784)
                     /gene="hfq"
                     /locus_tag="ACHURS_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFR1237416.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9870..10820)
                     /gene="miaA"
                     /locus_tag="ACHURS_001652"
     CDS             complement(9870..10820)
                     /gene="miaA"
                     /locus_tag="ACHURS_001652"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFR1237417.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10813..12660)
                     /gene="mutL"
                     /locus_tag="ACHURS_001653"
     CDS             complement(10813..12660)
                     /gene="mutL"
                     /locus_tag="ACHURS_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFR1237418.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12670..14007)
                     /gene="amiB"
                     /locus_tag="ACHURS_001654"
     CDS             complement(12670..14007)
                     /gene="amiB"
                     /locus_tag="ACHURS_001654"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFR1237419.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14026..14487)
                     /gene="tsaE"
                     /locus_tag="ACHURS_001655"
     CDS             complement(14026..14487)
                     /gene="tsaE"
                     /locus_tag="ACHURS_001655"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFR1237420.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14459..16006)
                     /gene="nnr"
                     /locus_tag="ACHURS_001656"
     CDS             complement(14459..16006)
                     /gene="nnr"
                     /locus_tag="ACHURS_001656"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFR1237421.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16005..17144
                     /gene="queG"
                     /locus_tag="ACHURS_001657"
     CDS             16005..17144
                     /gene="queG"
                     /locus_tag="ACHURS_001657"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFR1237422.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCIACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAQ
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17127..17180)
                     /locus_tag="ACHURS_001658"
     CDS             complement(17127..17180)
                     /locus_tag="ACHURS_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1237423.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17414..17489)
                     /locus_tag="ACHURS_001659"
     tRNA            complement(17414..17489)
                     /locus_tag="ACHURS_001659"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17454..17456),aa:Gly,seq:gcc)
     gene            complement(17525..17600)
                     /locus_tag="ACHURS_001660"
     tRNA            complement(17525..17600)
                     /locus_tag="ACHURS_001660"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17565..17567),aa:Gly,seq:gcc)
     gene            complement(17758..17833)
                     /locus_tag="ACHURS_001661"
     tRNA            complement(17758..17833)
                     /locus_tag="ACHURS_001661"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17798..17800),aa:Gly,seq:gcc)
     gene            complement(18044..18589)
                     /gene="orn"
                     /locus_tag="ACHURS_001662"
     CDS             complement(18044..18589)
                     /gene="orn"
                     /locus_tag="ACHURS_001662"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFR1237424.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            18684..19736
                     /gene="rsgA"
                     /locus_tag="ACHURS_001663"
     CDS             18684..19736
                     /gene="rsgA"
                     /locus_tag="ACHURS_001663"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFR1237425.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            19833..20801
                     /gene="asd"
                     /locus_tag="ACHURS_001664"
     CDS             19833..20801
                     /gene="asd"
                     /locus_tag="ACHURS_001664"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFR1237426.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKICQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            20823..24146
                     /gene="mscM"
                     /locus_tag="ACHURS_001665"
     CDS             20823..24146
                     /gene="mscM"
                     /locus_tag="ACHURS_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFR1237427.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDKPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(24297..>25637)
                     /gene="yjeM"
                     /locus_tag="ACHURS_001666"
     CDS             complement(24297..>25637)
                     /gene="yjeM"
                     /locus_tag="ACHURS_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFR1237428.1"
                     /translation="LMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIW
                     MVSTSAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKI
                     ARITAVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVV
                     FAIFAYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVL
                     SNGSVNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFT
                     LCYSPLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASA
                     FFNKLTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIAMVVVVLVVT
                     FANVFTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
BASE COUNT         5928 a         7193 c         6501 g         6015 t
ORIGIN      
        1 tatcttacca gctatagaca aaaaaaccat ccaaatctgg atggcttttc ataattcaga
       61 cgaactagct gcgctgacga accgcttcaa ataagcaaat tccggttgca accgaaacgt
      121 tcagggaaga aacacttcct gccattggga tgctgatcaa ctcatcgcaa tgttcacggg
      181 tcaggcgacg cataccttca ccttccgcgc ccatcaccag cgccaagcgt ccggtcattt
      241 tgctctgata gagtgtatga tccgcctcgc ctgccgtacc gacgatccag atattctctt
      301 cctgcaacat acgcatggtg cgcgcaaggt tagtcacccg aatcagtgga acgctttctg
      361 ccgcgccgca ggctactttt ttcgccgtag cgttgagctg tgcggagcga tctttcggca
      421 caatcaccgc gtgaacgcca gcagcgtccg cgctacgcag gcacgcgccg aggttgtgcg
      481 gatcggttac accgtcgagg atcagcagga acggttgatc gagcgaagcg atcagatccg
      541 gcagatcgtt ttcctgatac tgacgtcctg gcttcacgcg ggcgataatg ccctgatgca
      601 cggcaccgtc gcttttctcg tcgagatatt ggcggtttgc caactggata accacgccct
      661 gagactcaag ggcgtgaatc agcggtaaca gacgtttatc ttcacggcct tttaaaataa
      721 agacttcctg aaaacgttca ggggcgcgct ccagcagggc ctgcactgcg tggatgccgt
      781 aaatcatttc gctcattaat gtactcgttg tttacgtttt gcctgatgca ctacgtttat
      841 ccggcctaca tgatctctgc aatatattga atttgcgtga ttatgtaggc cggataaggc
      901 gttcacgccg catccggcat gaacaaagcg cattttgtca gcaatctaac cctcttcttt
      961 taaagagggt attgatcact ctgccacttt tttcttcgcc gcacgcttcg ctttggtcgc
     1021 tgcggctatt ttctgcgttt tcgccgatgg ctttttcgct tttctcgcgt ctttcttcgc
     1081 cgctttcggc ttcgtttttt tctcaccgcg gaaggcgctg tctggctcaa agtttacctt
     1141 tttaccgacc tgacgacgct tgccgccttt tttgcctgca tcgccttttt tcgctttctc
     1201 gcgcgccgtt ttaccgacgt tgcgcggtgc gcgttcactg gagatcagac taaagtcgat
     1261 tttgcgctcg tccatattaa ccgcttcgac gcgaacttcc acgcgatcgc ccaggcgata
     1321 agtctggccg ctggattccc ccatcaggcg ttgccctacc tggtcaaagc gatagtagtc
     1381 attgtccagc gaagagacat ggaccagacc atcaatgaac aagtcgtcca gacggacgaa
     1441 gaagccaaag ccagtgacgc tggaaattac gcctttaaag acgttaccta cctggtcgag
     1501 catgaagtca cacttcagcc agtcagcgac atcgcgcgtt gcttcgtcgg cacgacgttc
     1561 cgccatcgaa cagtgctgac ccagttgcaa catctcttcc atcgaataat ggtagccgcc
     1621 ggtttcagtg gtgttgccct gatgcccctg ctctttcgcc agcagatact taatggcgcg
     1681 gtgcagcgtc aggtcaggat aacgacgaat cggcgaagta aagtgcgcat aggactgcaa
     1741 tgccaggcca aagtgaccac ggttttctgg atcgtaaatc gcctgtttca tcgagcgcag
     1801 cagcatggtt tgcagcattt ctgcatcagg acggtcggca accgactcca gcagttccgc
     1861 gtagtcacgc ggttccggct tgttaccacc cggcagctcc agccccagct ccgccagcac
     1921 tgaacggaaa gaggtaatcg cttcggtgct cggcttgtcg tgaatacgga acagtgccgg
     1981 ttctttcgct ttctcaacga aacgcgccgc cgagatattc gccagaatca tgcactcttc
     2041 aattaatttg tgcgcgtcgt tacgctgggt ctgttcgata cgttcaatac ggcgttcagc
     2101 gttgaaaatg aacttcgctt cttcgctctc aaatgagatc ccaccgcgtt cttcacgggc
     2161 tttatccagc actttataga ggttatgcaa ctcttcgaga tgcttaacca gcggggcgta
     2221 ctgctcgcgc agatcctgat cgccctgcag aatatgccag actttggtgt aggtcagacg
     2281 cgcgtgagag ctcatcaccg cttcgtagaa tttgtagccc gtcaggcggc ctttcgacga
     2341 aaccgtcatc tcgcacacca tacacaggcg gtctacctgc gggttgagcg aacacaggcc
     2401 gttagagagc acttccggca gcatcgggat aacctgcgaa gggaagtaca ccgacgtgcc
     2461 acggttacgc gcttctctgt ccagcggcgt tggcggacgc acatagtagc tgacgtcggc
     2521 aatcgcgacc cataaacgcc agccgccgcc gcgttttttc tcgcagtaaa ctgcatcgtc
     2581 aaagtcacgg gcgtcttcgc catcaatggt gaccagcggt aaatcgcgca aatcgacacg
     2641 gcccgctttt gcttcttccg gcacttcttc tttcagcccc gcaacctgtt gctcaacagc
     2701 ctgcggccag atatacggaa tttcatgggt acgcagagcg atatcaaccg ccatgccggt
     2761 gcccatattg tcgcccagca cttcgacgat tttacccacc gctttggtgc ggcgagtcgg
     2821 acgctgagtc agttcgacta cgaccacaaa gcccatccgc gcgcccatga tctgatcggg
     2881 cgggattaag atatcgaagc tcagacggct atcgtcagga accacaaagc cgacgcccgc
     2941 ttcggtaaag tagcgaccaa caatctggct ggtttttggc accagtacgc ggacaatacg
     3001 cgcttcacga cgacctttac ggtcagcacc cagcggctgc gccagcacct gatcgccatg
     3061 aatacaggtt ttcatctgct cgctggagag atacaaatca tctttacgcc cttcaacccg
     3121 cagaaagccg tagccatcac ggtggccaat aacggtacct ttcaccaggt cgaggcgttc
     3181 cggcagcgca tagcactgac gacgagtgaa gaccagttga ccatcgcgct ccatcgcgcg
     3241 caggcggcga cgcaggcctt caagctgctc ttcgccttca atgtgcagtt ctaccgccag
     3301 ctcatcacgg ctggccggtt tttcacgttt ggttaaatgt tcgaggataa attcccgact
     3361 agggatggga ttcgcgtatt tttcagcttc gcgttcctgg aaaggatctt gtgacatctc
     3421 ggttcctccg ttgtcatctc tgatgaagat tttcgtcact ccaccagcaa taatttataa
     3481 agcggttgat tctcttcaac caaatcggca agcgtgtagt tatccagttc cgtaagaaaa
     3541 ctttgcacgg ccttagaaag cgcctgtttc aaccgacagg caggtgtaat gtggcaaaac
     3601 tcactgctgc aattcaccag cgataagggc tccagctcgc gcaccacatc accaatacgt
     3661 atcgcactcg ccggtttacc caggcgaatg ccgccatttt ttccacgaac agcagtcacg
     3721 tagccggcac gactaagttg attgattatt ttgaccatat gattacggga gacgccgtag
     3781 acgtcagtca cttcagaaat actggtcatc cgcccttctg gcaatgacgc catgtagatc
     3841 agcgcacgta atccgtaatc agtgaaactc gttaactgca catcaacctc aaaagggaaa
     3901 tcgggaaaaa atacatttat attgatgata aaccagccac aagctgtgtc gctaatttat
     3961 ttcagttcgg ggaaggaaaa aagcgaggat ttaacactgt gtcggatagc gtaatggctt
     4021 aaccgacaca gcaatgtatc acactgttag cccggcaggc aaaatatctg ccaggcgtac
     4081 cagaattacg cgtcgaacgg atcgcgcaga atcatggttt cagtacgatc cggaccggta
     4141 gagatgatat cgatcggcac accggtcagc tcttcaatac gcttgatgta gttcagcgcc
     4201 gcctgcggca ggccgctacg atctttcacg ccgaaagtgg attcagacca gcccggcatg
     4261 gtttcgtaaa tcggctctac acctttccag tcgtcagctg ccagcggagt ggtagccact
     4321 tcgcgaccat ccggcatacg gtaagccacg cagagtttaa cctctttcag gccatccaga
     4381 acgtccagct tggtcaggca gaagccagac agggagttca gctgtaccgc acgacgaacg
     4441 gcaacggtgt ccagccagcc ggtacgacga cgacgacccg tagttgcgcc gaattcgtta
     4501 ccctgcttgc agaggaactc gccagtttca tcaaacagtt cggtcgggaa cggacctgca
     4561 cctacacgag ttgagtaagc tttgaggata cccagaacat aatcaacata acgcgggccc
     4621 aggccggaac cggtcgccac gccaccagca gtggtgttgg aagaagttac gtacggataa
     4681 gtaccgtggt cgatatccag cagcgtaccc tgcgcacctt caaacatgac gaaatcgcca
     4741 cgctgacgcg cctggtcgag caggtcagaa acgtcaacca ccatagaagt caggatgtcg
     4801 gcaacagcca tcgtatcatc cagaactttc tggtaatcaa ccgcttcagc tttgtagtag
     4861 ttaaccaact ggaagttgtg atattccatc acttctttca gtttttcagc gaaggtttct
     4921 ttgtcgaaaa ggtcgccaac acgcagaccg cgacgtgcta ctttatcttc ataagcaggc
     4981 ccgataccac gaccggtggt gccgatcgct ttcgcgccac gcgctttctc acgcgcgtta
     5041 tccagcgcaa cgtgataatc aaggatcagc ggacatgctt cagacagcag cagacgctca
     5101 cgaacgggga tgccacggtc ttccagttct ttcatctctt tcatcagcgc ggccggagac
     5161 agcacaacac cgttaccgat gatgctggtt acattctcgc ggagaatacc tgatggaata
     5221 agatggagaa cggttttttc accgttgatt acgagagtat ggcctgcgtt gtgaccgccc
     5281 tggtagcgta caacatattt agcccgttca gtcagaagat cgacgatctt acctttacct
     5341 tcgtcacccc attgggtgcc cagtacgacg acgttgttac ccatttttca aaatcaccgt
     5401 ttgcttaaaa atggattcta ccatcgcttt ttcagagtta cagcactttt tgcactcaaa
     5461 aatgacctcg atcagtctgt tttttgttca gccaatcgtt ttcctcaaca tgtagtagac
     5521 cacaacgccc gcaaccacaa gtccaccgcc aaaacgacgt aaaatattat cgggcaaatt
     5581 ggtcatcgca gagatcatct tcttccatgc cttcgggtaa agcatcggcc ctaaaccttc
     5641 cagtaccaaa accagggcaa gcgccagcca gattgtcgaa ttcattcatt atccttatag
     5701 aaaaagaaaa ccaccgacat ccctgaggat gcggtggctt tattgacctg taccgcagtc
     5761 gttatattaa cgcgttgcgg aagtcggcgt cttcatgtag cggaagaaat cgctatccgg
     5821 gctcatgacc atcacgtcct gattgccaga gaagctgttc tcataagcac gcaggctacg
     5881 gatgaatgcg tagaagtccg gatctttact gaatgcatca gcaaacagtt tggctgcttc
     5941 ggcatcgcct tcaccacgca tgatgcggcc ctgacgctca gcttctgcca gcgttctggt
     6001 cacttcatag tcggcagtcg cgcgcagttt ttccgcttct tcctgacctt gtgaacggtg
     6061 acgacgcgct accgcttcac gctcggcgcg catacggttg tagatcgctt cagacacttc
     6121 ggtcggcagg ttgatctgct tgatacgcac atcgacaact tcaataccca gcgccgccat
     6181 actgttcggg ttgatgaccg gaactttgcc cttcgtctca gccgttacgc gctctgccgc
     6241 ttcggcaatg gcgttatctg ccgccggggt agtaacttca tcttctgtac ccgcagaacc
     6301 ggagttcagc gcgtcacgta cttcgagggt cagacgacca cgggaatcgg tgacgatgtc
     6361 tttcacgtcc aggcgaccaa tttcagaacg cagacggtca gagaacttac gtttcaacag
     6421 cacttccgct tgcgaaatgt cgccaccacc cgttgccagg tagtaacggc tgaaatcgct
     6481 gatgcgccat ttgatgtaag aatcgacgat caggtctttc ttctctttgg tcacaaagcg
     6541 gtcggcctgg ttgtccatgg tctgaatacg tgcgtcgagc attttcaccg tttcaatgaa
     6601 cggtatcttg aaatgcagac ccggctcata aaccagaggt ttgttgtcat cgtcacgcag
     6661 taccttacca aaacgcagcg taataccgcg ctcaccttct ttgacgacaa agacagacat
     6721 gtaaagcact accagcacga tgatgataat cgcgataact gacttacgca tcgttattcc
     6781 ccctgacgct ggtagtcgtt acgctgcgcg ttggcgcggc gttggtccat aatatcgccc
     6841 tgactggtgg acgacgtgtt gcttgctcca ctggttgtgg aggaagaggc tggcggcaga
     6901 cgcagcagat tgctggcacc gttatcgctc ttcgccgcag gggcgttacc gcctttcagc
     6961 atctggtcta acggcagaac catcaggttg ccacctttat cgttaaccag cactttgcgg
     7021 gtgttaccca acactttttc catcgtctcg atatacagac gctcgcgagt aatttccggc
     7081 gcggctttat attccggcag aagtttagca aagcgcgcca cttcaccctg agcttccagg
     7141 atggtctggg ccttgtacgc acgcgcctct tcgaggatac gttgcgcctg accgttcgca
     7201 cgcggctgaa cttcgttggt atacgcttct gcttcacgaa tgtattgctg ttcgttttca
     7261 cgcgcggcaa tcgcatcgtc aaacgccgct tttacttctt ccggcggacg agctgcctgg
     7321 aagttgacgt ccagcagagt gatacccatg tcatacggac ggatagtctc ttccagttcg
     7381 cgctgagtat cgctacgaat cacggtacga ccttccgtca gaatgcggtc catggtgtat
     7441 ttaccgataa ctccacgcag ggcgctgtcg gtagcctgac gcaggctgtc atccgggctg
     7501 gtcacgctat acagatattt ttccggattg gtgacgcggt actgcacgtt catctcaacg
     7561 cgcactacgt tctcgtccga cgtcagcatc acaccagaag cggccagttc acgcacggct
     7621 tccacgttca ccggtttgac ttcgtcgata aacgtcggtt tccagttcag acccggctca
     7681 accagatggc tgaatttacc aaagcgtgtt accacgccgc gttcggcttc tttaatggta
     7741 tagaaaccac tggccgccca gataatgaca atcgctgccg ctgcgatggt aacgacacga
     7801 ccgccaagct gcgggcgcgg gccttgcgat gaactgccac cgccagatcc ggtgccttta
     7861 ccgccgccca gaccaccgag ctttttgctc agtttgcgga agatatcatc taaatcaggt
     7921 ggcccttgat cgcgaccgcc tttgtttcca tttccctcag agttgccgcc aggtttgctg
     7981 cttccccacg ggtcgcggtc ttgtccgtta ttaccgggct gattccacgc catgtttgtg
     8041 ctccatattt gttatgcggt gatccctgtt gtctttcgac gccagaggat atgactccac
     8101 gcttcagacg ctacgccgtt agatcaggta atcgatcaac gccggttctt gtttacagag
     8161 gcgacgccag tcaacgatcg gcatacgaac ttgcagactt acgctgccgt cctcctccat
     8221 ccactctttt tctattgcct gaagctgata aaaacgactt ctcagacgtc cttcctgcgg
     8281 cggcaaacgc aatgtatgct gcgccacctc gccggaaagc cgctccgtca aagcctgaaa
     8341 aagctgtggt atccccgctc cggtctgtgc ggaaagccag acacggatcg gtttgttctc
     8401 ttcgtcccga tcaatacgcg gttcgaaatc ttccagcata tcgatcttgt tcatcaccag
     8461 cagggttggg atctcgtgag cgtcgatctc ttcaagaacc gtattcaccg cttcgatgtt
     8521 ttcttgtaca cgtacatccg ccgcatcaat gacgtgcagc agtaatgtgg cttgccgcgt
     8581 ctcttgtaac gtggctttaa atgctgccac cagatcgtgc ggcaggtggc gaataaaccc
     8641 tacggtatct gcaagtacgg tttcaccgac atccgcgacg tcaatacgcc gcaacgtcgg
     8701 gtcgagggtg gcaaacaact ggtctgccgc gtagacccgc gcttcggtga tgcgattgaa
     8761 aagggtagat ttaccggcgt tggtatatcc caccagcgaa acagtaggaa cgtcggcttt
     8821 gatacgcgat tgccgcccct gctcacgctg cttttcaact ctttctaggc gcgactgtat
     8881 ctgcacgatg cgattacgca acaaacgacg gtcggtttcg agctgggttt cacccggacc
     8941 acgcaaacct atcccgcctt tctgtctttc aaggtgggtc cagccacgca ccaggcgcgt
     9001 agccagatgg cgcagctgcg ccagctcaac ctgcaactta ccctcatggg tacgcgcacg
     9061 ttgggcgaaa atatctaaaa taaggccggt gcggtcgata acacgacact cgcacaaacg
     9121 ctccaggtta cgctcttgcg ccgggctcag ggcatggtca aaaagaacga ccgaagcacc
     9181 cgtcgctttg acagcttccg caatttcaac tgctttacct tcacctacaa aatactttgg
     9241 gtgcggcgct ttacggctac cggtaatcac ctgcaatgct tcgacaccgg cggaagagac
     9301 cagagattca aactcctgga ggtcttccat atctttgtct tgcgtaaaat agatgtgtac
     9361 cagtaccgcc tgctcaccag catcataacg gtcaaacaag cgtataaccc tctaaataga
     9421 tcagcgggga acgcaggatc gctggctccc cgtgtaaaaa aaacagcccg aaaccttatt
     9481 cggtttcttc gctgtcctgt tgcgcggaag tattctgcgc gctgctacca tgatggtagt
     9541 tactgctggt accgccaccg gcgttgttac tgtgatgaga aaccgggcga gacgggacaa
     9601 cagtagaaat cgcgtgcttg taaaccatct ggctgaccgt gtttttcaac aggatcacga
     9661 actgatcaaa agactcgatt tgcccttgca gcttaatacc attcaccaaa taaatagaaa
     9721 ctggaacacg ttcccgacgc agtgcgttca ggaacggatc ttgtaaagat tgccccttag
     9781 ccattctctc ttttccttat atgcttattt gtactttgaa cctttcgatt ctgaaaaaat
     9841 tgcgcacgat acgtctcaat tgtacacatt cagcctgcga tagcaccaac aacctgtaat
     9901 acttcgtcac gcgcctgttc tggtttttca ctgtcaagcc agtgaacccc ttcccaacca
     9961 cgcagccagg ttatctgccg cttcgccaac tgtctcgtgg cgcaaacacc tcgataaacc
    10021 atttcatcgt atgagatttc gccttcaagg taagaccaca tctggcgata acccacgcaa
    10081 cgaatggaag gcaagtccgt atgcaaatct cctcgggcaa aaagcgcccg gacttctgct
    10141 tcaaaacctg aagccaacat ctgatgaaaa cgctgctcaa tgcgttgatg gagcagttca
    10201 cggctcgccg gggcgatggc gaactgatgc acctgatacg gtagagcgtc tcctgacgtt
    10261 tgcgtcagtt ccgttaaagt tttacccgaa atgaaaaaaa cttccagtgc ccgggaaagc
    10321 ctttgtggat catttggatg aatccttgct gccgcaaccg gatctacctc ctgaagttga
    10381 cgatgcaatg actcccaacc ttgctctgcc gcctgttgct caattctggc ccgcacttcc
    10441 gggtctgccg acggtagcgg cgacaaccct tccagcaatg ccttgaaata caacatcgta
    10501 ccgcccacta acagtgggat ccgccccgcc gcggtgatat cggccatttc cgccagcgca
    10561 tcgcggcgaa aatcagcagc cgagtaagcc tgcgacggat cgcgaatatc cagcaatcgg
    10621 tgcggcgcgg cgagtaactc ttcagcgttc ggcttcgccg tcccgatatc catccctttg
    10681 taaataaggg cagaatcaac gcttatcaac tctactggta aaattttacg cagctcaatg
    10741 gctaacgccg ttttaccgga ggccgtcggc cccatcaaaa aaatcgcctt aggcaggctc
    10801 gccttactga tatcactcat ctttcagggc ttttatcgcc ggatgtaaat caacagattg
    10861 taacagacca cccggcggcg ttttcacaag ttgcggacat aaccgttcca cgtccgccag
    10921 cagggttatg gcctgtgcca ttgaccactg cgcatgttcg ctcatcagat ttcgtgcaat
    10981 ccactgcgca atattgccag gttcgaatac ggactgcttc gccaggtagc ctatcagttc
    11041 aggaatcaag atttgtaaat tttgttggcg taagggtaaa ggcacggccc tgatggtcac
    11101 atgctgtgca tctgactgga aatcaatacc caattccgcc agggcagact gcgctttttc
    11161 taatgccgat ttttcttcgc cagaaacttt tagccgcaac ggaataagca gcggctgagc
    11221 gcaaactggc gcttcacccg gcgtcagttg tacctgacgc agccaacgtt ctgccactgg
    11281 caaggctaaa agtgaaatgt tgccgtcgcg ctccagcaac gcacagtcgg aatggacgat
    11341 agtcagtacc cgaccaaaac tctgactgtt cgccgcaagt gcaggttcct gcggttccgg
    11401 cgcttttaat ttttgcatcg gcgcgggcgt ttgcaaaagc tggcgataca cttcaccttg
    11461 ctgtttctgg tagcctggct gcgcattcgg ccagggggca gccggacgac tgcctgatgc
    11521 tggcgcagga gtgtagcgcg gagctaccgg ctcacgagct gccggttctg caaagtgatt
    11581 gcgccccgcc gccacgcggt tttccggaat ggaacgcggt gcaggttggg gttcatcgtc
    11641 cagcggtagc ggcgtttcca gttgctgttg tagcacgctc agcacgccct gatagataaa
    11701 atcatgcacc agacgcgact gatggaaacg cacttcgtgt ttggcggggt gcacgttgac
    11761 gtccacctga tgcggatcga tctccagata cagcacaaat gccggttgct gatcggcccc
    11821 cagtttgtct tcgcaggcct ggcggatcgc gtgattgatc aggcgatcgc gcatcatgcg
    11881 accgttcacg tagcaatact gaatttctgc cagtgcgggc gtggtgtgat ttggatcggc
    11941 cacccagccg cgtagcgtga gatcgccgtg ttgccattca atcgccagcg cttgttcaag
    12001 aaaagcggtg ccgcaaatcg cgcctaagcg ccgttctttt tgcccgcctt ccggcactgc
    12061 gcggtactga cgcacaattt taccgttatg cgacaggttg atcgtgacgt cgaaacgcgc
    12121 cagcgcaatg cggcggatga tctcatcaat gtggttaaat tcggttttct cggtgcgcag
    12181 gaatttgcgc cgcgccgggg tgttgtagaa cagatccagc acctccagcg tcgtccccac
    12241 aggatgcgcc gccggtttta ccgtcacgtt catatcgcgc ccttcggcat aggcctgcca
    12301 ggcttcctgc tgttctgcgg tgcgtgaagt gagcgtcagg cgggaaaccg aactgatact
    12361 cgccagcgcc tcaccgcgaa agcccaggct gataatggct tcgagatcgt ccagagaggc
    12421 gattttactg gtggcatgac gagccagcgc cagcgccagc tcatcttttt tgataccgca
    12481 gccgttatca cgaatgcgga taagtttcgc cccaccgcgt tcgatatcaa tatcgatacg
    12541 cgtcgcacct gcatcgaggc tgttttccac cagttctttg actaccgacg caggtcgctc
    12601 gaccacctca cctgcggcaa tctggttcgc cagttgtggc ggtaagacct gaattggcat
    12661 caatcgtcct tagtttggca gcgtgcgatc tggcgtcgtc accgtactgg cagtttgtgc
    12721 cgttgccccc tgcggcgcag attgcatcgg atgcgcaagg aaataattgc gcaggccttt
    12781 gtaaatggct tctgccagct gttgttggta atcgtcgctc gccagcaaac gttcttcgct
    12841 gttgttgctg ataaaaccgg tttcgaccag tactgacggg atatccggcg aacgcagaac
    12901 gccgagactc gcgtgttctg gtcggcgttt atgtatttcg ccaatgcgtt gcaactgact
    12961 gatcatactg gtcgctacat cataccctac ccgctgggaa tgaccgaact gtaaatccag
    13021 caccgcctgg cttaaatagg ggtcagactg actgttcgcc agcacatcgc ccgccccacc
    13081 cagcagctcc gactgtttct cgtgctgctc cagccagctg gccatttcgc tgttggcgcg
    13141 acggttagag agcacccata cggaagcgcc agtcgcactg cggttcggtg cggcatcagc
    13201 gtgaatcgac acgaggaaat tggcgttttg cttacgtgcc acatcgctgc gccccatcac
    13261 cgagataaag taatccccgt cacgggttaa aacgccttta aacatcggat cgtcattgag
    13321 caaagtgcgc aatttacgcg cgatggcgat ggtgacattt ttctcccgcg taccaccggg
    13381 gccgatagcg ccagggtcct gaccgccgtg tccggcatca atagcgatga taattttatc
    13441 gccagtgtta gccgtcgcgc gcgctgccgg acgcgttacc gtattactgc tgataacacc
    13501 cgtagtgcgg ttactttccg ttttaaacgg attgcgcgcc ggttcgctga cgcgcggtgc
    13561 gacaaccgca ggcgtttcaa cgcgtttcgc aaccacgggc ggtggaggag gcggtggcgg
    13621 cacatcggcg ttaatcgtaa agacgacagt gtaattgctg ccattctgcc gcttcaccgc
    13681 ttcggtttta ccgttttcgg taagatcgac caccagccgt agcgtttgtg catctttagg
    13741 cgttccagag cgaatcgcct tcaccagatt attgccgctg aacaacaacg gcagtccctg
    13801 aatcacgccc gtttgtttga tatcgagcgc cacggtgcgt ttgctttgat ggctaaacgc
    13861 ataatcagga tcgccaataa aactcaacgt tatccgcgcc tgttgattac cgttagaaac
    13921 ctgaatatca gagagcgtcg cggcacccac cggcgtgcac agcagcagca gcgtcgctac
    13981 caaccaattt ctgatgcgat acatcatccc gccacctttc aaaggttaac cggctaaacg
    14041 cgccagcaac aattcacccg cagaggaaac cgcactcacg cgcgcctcac ggccttgtgc
    14101 ctgataatca atgtgtattt cgacatccgg gtcaggaaga acacctgtac cttgttgtgg
    14161 ccactccacc aggcagatgg catcgttggc aaaataatcg cggatcccca taaactccag
    14221 ctcctcggga tcggcaaggc ggtacaaatc aaagtgataa accattaaat tgtcgagcgt
    14281 atagggttcg accagcgtat aagtggggct tttgacatta ccctgatgac ccagagcttg
    14341 taaaaaaccc cggctaaagg tggttttacc tgcgcctaaa tcgccataca gatagattac
    14401 ggttgcgcca tcgcaggctt tcgctacccg ctcgcccagg tctaatgttg cctgctcatc
    14461 agggagcgga attactcgat tcatcatggt ttttatcagt cacttccggg ttaacaatac
    14521 gctgtagcgt ggaaaagaga tcggttgcca gcatcccgcg cgttccaaaa cgcgccgcca
    14581 gtacgtcagc tgccgcaccg tgcgcgacac agcccgcaca ggcggcatca tacggcgaca
    14641 gtttttgccc aagcaatgcg ccaataatac cagagagcac atcgcccatg ccgccgctcg
    14701 ccatgcctgc atttccggca tcaataatgc ctaaagcgtc aggatgggcg gcgaccacgg
    14761 ttccggcacc tttcagcacc gctacgccgc cataacgttg taccagacgt ttggcgcaat
    14821 gtaagcggtc actttcaatt tcagcgacgg aacagcctaa caaccgtgcg gcctcgccag
    14881 gatgcggcgt gatcacgcga ttgtgacgct tatcgggatt gattgccagc aggttcaatg
    14941 catcggcatc ccacaacatc ggtttgcgaa aattctcaac tttttgcagt gcttttttcc
    15001 cccactcttg ctggcccaga ccgggaccaa tcaccaccac atcggcccat tccaggcttt
    15061 cggtaagaga gtccatcgtc agttcatgca ccatcaattc cggtcgtgca gtcagcagcg
    15121 gcgcaatgtt ttcactgcgg gtcagtactc ggactaaacc agcaccagca cgcagcgccg
    15181 cttcccccgt catacgaata gcccccgccg tgccgtgatc gccaccgata atcaccagcc
    15241 gcccgtgatc gcctttatgc gaagtcgggc gacgcggttt cagccagtga gaaagttgtt
    15301 ctgccgaaaa ccgctgaatt ttcgtctcct gacctgccag ccagctatcc agccccagtg
    15361 agtcaaaatg cagttgtcct gtaacatccc gcgcttttcc agtgagcaag cctggtttca
    15421 gcgcaataaa agtgatggtg tgatcggcgt tgatcactgc gcctggcgta gcgccggttt
    15481 cagccagcag gccggaaggg atatcaaccg ccacaatcgg cgcaggatgg gaattagcgt
    15541 ggtcgattaa ctggctaatg gattcgcggg gcgcttgctg caagccggta ccgagcagcg
    15601 catcaacaat cagatctacc gattcgggcc agacaatatt cgaagcatgg atctcgccac
    15661 ccgcgtttaa ccatgcttcg cgtgccagcg cggcctcttc cggcaacggt ttgtcgctct
    15721 cctgggccaa caacgtgacc tcaatgccga ccgctttggc cagtcgcgcg accacgtagc
    15781 catcgccgcc gttattacca tgaccgcaca gcaccagcca gtggcgggcg tcaggatacg
    15841 ccgaacgaca cacctggaat gcggcctcgc cagcgcgaag catcagctca tagagtgtga
    15901 gccccagcac atctgccgcc tcgcgttctc cgcggcggat atcgtcggcg taccagacgg
    15961 tgtgtggtat acttacgggg tttttcttca ttgtatggtc cgtcatgtca gagcccctcg
    16021 atctcaatca gttagcgcaa aaaattaaac agtgggggct ggaactgggc tttcagcagg
    16081 taggtattac cgataccgat ctcagcgagt ccgagcccaa actgcaagca tggctggaca
    16141 aacaatacca cggcgaaatg gactggatgg cacgtcacgg tatgctgcgc gctcgccccc
    16201 atgagttatt accaggtacg ctgcgtgtaa tcagcgtgcg gatgaattac cttcctgcta
    16261 acgccgcatt tgccagcacg ctgaaaaacc ccaaactcgg ctatgttagc cgttatgcgc
    16321 tgggccgtga ctatcacaaa cttctgcgca accgactcaa aaagctgggc gagatgattc
    16381 agcaacattg tgtttcgctg aattttagac cgtttgtcga ttctgcgcct attctcgagc
    16441 gcccgttagc tgaaaaagct gggctcggct ggacaggtaa gcactcactt atcctcaatc
    16501 gcgaggccgg ttctttcttc tttttaggcg aattgctggt cgatattccg ctgcccgtgg
    16561 atcaaccagt cgaggaagga tgcggtaaat gcatagcctg tatgacgatt tgcccgaccg
    16621 gtgccatcgt cgagccatat accgtcgatg ctcgccgctg tatctcttat ctcaccatcg
    16681 aacttgaagg ggcgatcccg gaagagttgc gaccgttaat gggaaaccgt atttacggtt
    16741 gcgatgactg ccagcttatc tgcccgtgga atcgctattc gcaactcact acagaagacg
    16801 atttcagccc gcgtaagccg ctacacgcac aggaactcat tgagttattt gcctggagcg
    16861 aagagaagtt tttaaaagtc acggaaggat cggcgattcg ccgtataggt cacctgcgct
    16921 ggctgcgtaa tatcgccgtg gcattaggca atgcgccctg ggatgaaacg attctggcgg
    16981 cgctggagag tcgcaaaggt gagcacccac ttcttgatga gcacatagcg tgggcgattg
    17041 cgcagcaaat cgagagacga aatgcgtgca tagtcgaagt gcaattgccg aaaaaacagc
    17101 gtctggttcg ggtgattgaa aaagggttac cgcgtgacgc ctgattcatc cacagcttgt
    17161 gaataaaaat aaaaacgcat tgcaattcaa gacgcataaa atcgtcaagt gatcgactcg
    17221 acaaaacaga atcttgattt ttaacttatt tatcaatagg ttaaaaaaat accattcacc
    17281 gtgcgaagtt tctttgaaat taagattaca gcctgaggat gtggataact ctgtttacaa
    17341 gagtttgtca gagacaacaa aaaccagcaa taacgcatcg cgtcgctgtg gatattttat
    17401 tgagagaaga atttggagcg ggaaacgaga ctcgaactcg cgaccccgac cttggcaagg
    17461 tcgtgctcta ccaactgagc tattcccgct tgggtggtct gtgccttaca gcactttcaa
    17521 attttggagc gggaaacgag actcgaactc gcgaccccga ccttggcaag gtcgtgctct
    17581 accaactgag ctattcccgc ttaatcttcg tctttcgaac cgctactgcc ttggctgcct
    17641 tcactcacct tagtcacata cttttgtaag ttcccaagga ttcgttcagt tgccgccttg
    17701 tcgcaattcg aaatactgcg attgggtggt ctgtgctttg cagcactttt caaattttgg
    17761 agcgggaaac gagactcgaa ctcgcgaccc cgaccttggc aaggtcgtgc tctaccaact
    17821 gagctattcc cgcgtactac ttaattttgc tttcgtaatt ttcgcatttc tgcgtcgtta
    17881 cgggaggcgc attatacgag aaatctttcc ctctgcaacc ccccaaaaaa gttttttttg
    17941 cgtttttatc tcaagtgctg aaattatcag caagacgttc aatttagcgt caaaatcacg
    18001 ccgccgcaag tggattccgg cgcttacgtg accaggaaaa attttacagc ttgataaaat
    18061 gctcgcggta gtaagccagc tccgccaccg attcacggat atcatccatt gcctgatgcg
    18121 tcccctgctt ggtaaaacca tccagaattt ccggcttcca gcggcgcgcc agctctttca
    18181 gggtgctgac atcgagataa cggtagtgga agtaggcttc cagctccggc atgtatttaa
    18241 acaggaaacg acggtcctgt ccgatgctgt taccgcaaat cggcgatttt cccgcaggca
    18301 cccactgttt taagaattcg agcgttgcca gttcagcttc ccgatcgccc atcgtgctcg
    18361 ctttcacgcg ctctaccagc ccgctggcgg tatgggtgcg cacgttccag tcatccatca
    18421 gcgccagctg ttcatcggac tgatgtactg caatggtcgg cccttctgcc agaatattca
    18481 gattggcatc ggtcaccagc gtggcaatct caataatgcg atcgcgctcg ggatccagac
    18541 cggtcatctc aagatcgatc caaatcaggt tgttttcatt ggcactcatg ctattttcca
    18601 cccattcaca gtgactatat tgatccacaa ttgcgtgtat catagatgtt ttgcccatca
    18661 ggggcgacca ggagtcagta cgattgagta aaaataaact ctccaaaggc cagcagcgcc
    18721 gcgtgaacgc caatcaccag cgtcgtctta aaacgtctaa ggagaagccc gactacgacg
    18781 acaatctgtt tggcgagcct gatgaaggta tcgtcatcag ccgctttggt atgcacgctg
    18841 atgtggaatc cgccgatggc gacgttcacc gctgcaatat tcgccgtacc atccgttcgc
    18901 tggtaaccgg cgaccgcgta gtctggcgtc cgggtaaacc ggcggcggaa ggcgtaaatg
    18961 tcaaagggat cgtggaagcg gtgcatgagc gtacctcggt gttgacgcgt ccggatttct
    19021 acgacggcgt aaaacctatt gccgccaaca tcgaccagat tgtcattgtc tccgccattt
    19081 tgccggagct gtcgctcaat attatcgacc gttacctggt ggcctgcgaa accttgcaga
    19141 ttgagccgat tattgtgctc aacaagatag acctgctgga cgacgaaggc atggcgttcg
    19201 tcaacgagca gatggatatc taccgcaata tcggttatcg cgtattgatg gtttccagcc
    19261 atactcagga tgggctaaaa ccgctggaag aggcgttgac cgggcgcatc agcatttttg
    19321 ccgggcagtc tggcgtcggc aaatccagcc tgctgaacgc gttgctgggg ctgcaaaaag
    19381 agatcctgac caacgatgtc tctgacaact cggggcttgg tcagcacacc acgaccgccg
    19441 ctcggctgta tcacttcccg cacggcggtg atgtgattga ttcccctggt gtgcgtgagt
    19501 tcggcctctg gcacctggag ccggaacaaa tcactcaggg ctttgtcgaa ttccatgact
    19561 atttaggtct gtgtaaatat cgcgattgca aacacgatac cgatccgggc tgcgctatcc
    19621 gggaagcggt tgaggaaggg aaaatcgcgg aaacccgttt cgaaaactat caccgtattc
    19681 tggaaagcat ggcgcaggta aaaacgcgta aaaacttttc tgatacggat gactgacaat
    19741 taagctaacc gtcgttagaa tcgtcccctt tttttcagga tccggcatgt aggccgaatc
    19801 aggaacgaca aaacaatggc ctggaggcta ccttgttaaa ttcatttaaa ctttcgctac
    19861 agtacattct gccgaaacta tggcttactc gcctggcggg ttggggcgca agcaagcggg
    19921 caggatggtt gacaaaactg gttatcgatc tgttcgttaa atactacaag gtcgacatga
    19981 aagaggcgca aaagccggac accgccagct accgcacctt taacgaattc tttgtccgtc
    20041 cgctgcgtga cgaagtacgc ccaatcgata ccgatccgaa tgtactggtc atgcctgccg
    20101 atggcgttat cagccagttg ggtaaaatcg aagaagataa aatcctgcaa gccaaaggcc
    20161 acaactacag cctcgaagcc ctgctggcag gcaactatct gatggcggac ctgttccgca
    20221 acggtacgtt tgtgaccact tacctctccc cgcgtgacta ccaccgcgta cacatgccgt
    20281 gcaacggtat tctgcgtgag atgatctacg tgccgggcga tctcttctcc gttaaccatc
    20341 tcacggctca gaacgtgccg aatctgtttg cccgtaacga acgcgtgatt tgccttttcg
    20401 ataccgaatt tggcccaatg gcgcagattc tggtcggagc gacgattgtt ggcagcattg
    20461 agacggtctg ggcgggcacc attacgccgc cgcgcgaagg tatcatcaag cgctggacct
    20521 ggcctgccgg ggaaaacgac ggttctgtgg cactgctgaa aggccaggaa atgggtcgct
    20581 ttaaactcgg ttccaccgtt atcaacctgt ttgcaccggg taaagtgaat ctggttgagc
    20641 aactggaaag cctgtctgtc acgaaaattt gccagccgct ggcagtatct accgaaacct
    20701 ttgttacgcc agacgctgaa cctgccccgc ttcctgctga agagatcgaa gcagaacacg
    20761 acgccagccc attggttgac gacaaaaaag accaggtcta atccatcaaa ggaaacgctg
    20821 acgtgcgcct gattatcact tttctgatgg cctggtgcct cagttggggg gcgtacgccg
    20881 cgacggcccc cgatagcaaa caaatcactc aggaactgga gcaggcaaaa gcggcgaaac
    20941 ccgcacagcc ggaagtcgta gaggcgcttc agtctgcctt aaatgcgctt gaggaacgaa
    21001 aaggttccct tgagcgcatc aaacaatatc agcaagttat cgataattat ccgaaactct
    21061 ccgctactct gcgcgcacaa ttaaacaaca tgcgtgacaa gccgcgcagc gtgtcgccgg
    21121 gaatgtctac cgacgcgctg aatcaggaaa ttctccaggt cagcagccag ttgctggata
    21181 aaagccgtca ggcccagcaa gagcaggagc gcgcccgcga gattgccgat tcgctgaatc
    21241 aactgccgca acagcaaacc gacgcccgcc gtcagttaaa tgagatcgag cgccgcctgg
    21301 gaacgcttac cggcaatact ccgctcaatc aggcacaaaa tttcgcgttg cagtctgact
    21361 ctgcacgtct taaggcgctc gttgatgaac tggagctggc gcagctgtct gccaataacc
    21421 gccaagaatt agcgcgctta cgctcagagc ttgcggaaaa agagagccag caactggatg
    21481 cgtatttgca ggccttacgt aatcagttga atagccaacg tcagcttgag gcggagcggg
    21541 cgctggaaag taccgaatta ctggcagaaa acagtgccga tttgccgaaa gatatcgtcg
    21601 cgcaattcaa aattaaccgc gaactatcgg cagcgctgaa tcaacaggcg cagcggatgg
    21661 atctcgttgc ctcgcaacag cgtcaggctg ccagccagac gttacaggtc cggcaggcgt
    21721 tgaatacgct gcgtgaacag tcgcaatggc tgggatcgtc caatctgctc ggtgaagcgt
    21781 tgcgagcgca ggtggcacgg ctaccagaaa tgccaaaacc acaacagctt gataccgaaa
    21841 tggcgcagtt gcgtgtgcaa cggttacgtt atgaggatct gcttaataaa cagccgctgc
    21901 tacggcaaat ccatcaggcc gacggtcagc cgctgaccgc cgagcaaaac cgtattctgg
    21961 aagcacaact gcgcactcag cgtgagttgc tgaactcatt gttgcagggt ggcgacacgc
    22021 tactattgga actgaccaag ctgaaagtct ccaacgggca actggaggat gcgctgaaag
    22081 aggtgaacga agcaacgcac cgctatctgt tctggacctc tgacgtgcgc ccgatgacca
    22141 tcgcctggcc gctggaaatc gcccaggatc tgcgtcgtct catttcgctg gacaccttca
    22201 gtcagttggg caaagccagt gtgatgatgc tgaccagcaa agagacaatt ttgccgctgt
    22261 ttggcgcgtt gattctggtc ggttgcagta tttactcgcg ccgctatttc acccgttttc
    22321 ttgaacgttc ggcggcgaaa gttggcaaag tgactcagga tcacttctgg ctgacgttgc
    22381 gcactctttt ctggtcgatt ctcgtcgcgt caccgttacc ggtgctgtgg atgacgctgg
    22441 gttacggctt gcgcgaggcg tggccttatc cgctggcggt cgcgattggc gatggtgtaa
    22501 cggccaccgt gccgctgctg tgggtagtga tgatttgcgc cacctttgcc cgcccgaacg
    22561 gcttgtttat cgctcatttt ggctggccgc gcgaacgcgt atctcgtggg atgcgctact
    22621 acctgatgag catcgggctt attgtgccgc tgattatggc gctgatgatg ttcgataacc
    22681 tcgacgaccg tgaattctcc ggttcgctgg gaaggctttg ctttatcctc atttgcggtg
    22741 cgctggcggt ggtcaccctc agcctgaaaa aggccgggat tccgctgtat ctcaacaaag
    22801 aaggcagcgg cgacaacatt accaaccata tgctgtggaa catgatgatt ggcgcgccgt
    22861 tggttgccat tctggcgtcg gctgtgggtt atctggcaac ggcacaggcg ctgttagcga
    22921 ggcttgaaac ctcggttgcc atctggttcc tgctactggt ggtttatcac gttatccgcc
    22981 gctggatgct gatccagcgc cgcaggctgg cgtttgaccg ggcgaagcat cgccgggcag
    23041 agatgttagc gcaacgtgcg cgtggcgaag aggaagcgca tcatcacagt agcccggaag
    23101 gagcaattga agtcgatgaa agcgaagtcg atctcgatgc catcagtgcg caatccttgc
    23161 ggctggtgcg ctcaattttg atgttgatcg ccctgctttc tgtcattgtg ctgtggtcag
    23221 aaatccattc cgctttcggc ttcctcgaaa atatttcgct gtgggatgtc acctccacgg
    23281 tacagggcgt agaaagtctg gagccaatta ccctcggtgc ggtgctgatt gccattctgg
    23341 tgtttatcat caccacgcag ctggtgcgca acctgcccgc gctgctggaa ctggcgattt
    23401 tgcagcacct ggatttaacg ccgggtacgg gctacgccat caccaccatc accaaatatc
    23461 tgctgatgct gattggcggg ctggtcggct tctcgatgat tggtattgag tggtcgaaat
    23521 tgcagtggct ggtcgccgcg ctcggtgttg gtctcggttt tggtttgcag gaaattttcg
    23581 ccaactttat ctctggtctg attatcctgt tcgaaaaacc gattcgcatt ggcgatacgg
    23641 tgacaattcg cgatctcacc ggtagcgtga cgaaaattaa cacccgcgcc accaccatca
    23701 gcgactggga ccgtaaagag ataatcgtgc cgaacaaggc gtttattacc gagcagttta
    23761 tcaactggtc gctctctgac tcggtcacgc gcgtagtgtt gacgattccg gcccctgctg
    23821 atgccaacag tgaagaagtg acggaaatcc tgctcaccgc agcgcgtcgc tgctcgctgg
    23881 tgatcgacaa cccggcaccg gaagtcttcc tggtggatct gcaacaaggg attcagattt
    23941 tcgagctgcg tatttatgcc gctgagatgg gtcaccgtat gccgctacgc catgagatcc
    24001 accagctgat tctggctggc ttccatgccc acggtatcga tatgccattc ccgcccttcc
    24061 agatgcgtct ggaaagcctc aacggtaaac aaacggggag aacgctgacg tctgcgggca
    24121 aaggtcgtca ggcgggaagt ttgtaatgca tgaagctgcc aaatgatgtc aacgtggctc
    24181 atttagcagc ttaacatcat cttcagttat caccggccct ggagagtatc atccatacgc
    24241 attacttcct gacaactcat cgccggagag acgatgagtt gcaggatttc atatgatcag
    24301 tcgagagcta actcaggttt attcgccatg cgactgcaat agttctggta aatcgccatc
    24361 gctaacagcg agaagaagac agggccgcca atcatccaca atgtgctgtc ccagtctccg
    24421 gcttcaacca caggctgaat aatggtgaag acgttcgcaa atgtcaccac cagtacgaca
    24481 accaccatcg caatcattgc cgacatacgc gttttgaaaa tcacaaatgg tctgtcgaga
    24541 tcctgacgtg ctttaaagaa tgggaaagcc agcgcgagga acaggtaagg aagcgtcata
    24601 gacacgttcg ccatcagcgt cagcttgtta aagaacgcgg atgcggtacc gccaccaaac
    24661 gaaaccagca ggatgaagac agtaaccaac ccgcactgca tccacatggc gatagacggc
    24721 atccccatcg cattcaggcg cgtcatcggt tccggccaca acgctttcgg cgtcccctgg
    24781 atgatggctt tcagcggtga atagcaaagc gtaaagaacg caccggtata agcgaggaac
    24841 atcgaaagtc cggtaatacg cgcaaaccat acgcccagcg acaatgacgc ttcaggtgac
    24901 aaatgcagtg cgttacccag cgtcaccccg aggctcttca tcagcacata ggtaatattg
    24961 ccaaggttaa cggaaccatt acttaatacc tgctgccagt ttgtgctgac gccccataaa
    25021 aatattgcca gcgaataacc gattgaaata acaatagcgg caaaaacaat acctttggca
    25081 aagttctttt ctggattttc cgttttatcg accagaccac caaccgcttc aattccgcca
    25141 taggcaaaaa tagcaaatac cacaaacgat agcattgcca gaccggactg ataacccggg
    25201 ttcggtgatg caaggaaatt aatatcctgc gcgaaatgcc caccatttaa taacaaaata
    25261 gtaatgctta ctaacagcaa tactaaattc agacacatta ctgcaatacc gcccaccgca
    25321 gtaatgcggg caattttatt aatccccttt gaagcaacga cggtgaccag aatcatccat
    25381 gctactgcca gcagaccaac cacctgcgta ggctccagtc cggcaatacg ccagtgctgg
    25441 gtcatgtcgc taccatagag gaatgttgag aacggtaccc aaactttcgc ggaggtactc
    25501 accatccaga tgatataaga ggaaaaccac ataaacgtac caatgaaggc aaaacgtggt
    25561 ccgacactat tattcatcca ggaatagata ccaccttctt ctttgcgata agcagctccc
    25621 atttcagcca tcattaa
//
LOCUS       DAZPDU010000044        25284 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZPDU010000044 DAZPDU010000000
VERSION     DAZPDU010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25284
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.044"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            17..268
                     /locus_tag="ACHURS_001667"
                     /pseudo
     CDS             17..268
                     /locus_tag="ACHURS_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="involved in type II protein secretion; incomplete;
                     partial in the middle of a contig; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            506..817
                     /gene="rpsJ"
                     /locus_tag="ACHURS_001668"
     CDS             506..817
                     /gene="rpsJ"
                     /locus_tag="ACHURS_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFR1237429.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            850..1479
                     /gene="rplC"
                     /locus_tag="ACHURS_001669"
     CDS             850..1479
                     /gene="rplC"
                     /locus_tag="ACHURS_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFR1237430.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     EVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            1490..2095
                     /gene="rplD"
                     /locus_tag="ACHURS_001670"
     CDS             1490..2095
                     /gene="rplD"
                     /locus_tag="ACHURS_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFR1237431.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            2092..2394
                     /gene="rplW"
                     /locus_tag="ACHURS_001671"
     CDS             2092..2394
                     /gene="rplW"
                     /locus_tag="ACHURS_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFR1237432.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            2412..3233
                     /gene="rplB"
                     /locus_tag="ACHURS_001672"
     CDS             2412..3233
                     /gene="rplB"
                     /locus_tag="ACHURS_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFR1237433.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            3250..3528
                     /gene="rpsS"
                     /locus_tag="ACHURS_001673"
     CDS             3250..3528
                     /gene="rpsS"
                     /locus_tag="ACHURS_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFR1237434.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            3543..3875
                     /gene="rplV"
                     /locus_tag="ACHURS_001674"
     CDS             3543..3875
                     /gene="rplV"
                     /locus_tag="ACHURS_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFR1237435.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            3893..4594
                     /gene="rpsC"
                     /locus_tag="ACHURS_001675"
     CDS             3893..4594
                     /gene="rpsC"
                     /locus_tag="ACHURS_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFR1237436.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            4607..5017
                     /gene="rplP"
                     /locus_tag="ACHURS_001676"
     CDS             4607..5017
                     /gene="rplP"
                     /locus_tag="ACHURS_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFR1237437.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            5017..5208
                     /gene="rpmC"
                     /locus_tag="ACHURS_001677"
     CDS             5017..5208
                     /gene="rpmC"
                     /locus_tag="ACHURS_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFR1237438.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            5208..5462
                     /gene="rpsQ"
                     /locus_tag="ACHURS_001678"
     CDS             5208..5462
                     /gene="rpsQ"
                     /locus_tag="ACHURS_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFR1237439.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            5627..5998
                     /gene="rplN"
                     /locus_tag="ACHURS_001679"
     CDS             5627..5998
                     /gene="rplN"
                     /locus_tag="ACHURS_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFR1237440.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            6009..6323
                     /gene="rplX"
                     /locus_tag="ACHURS_001680"
     CDS             6009..6323
                     /gene="rplX"
                     /locus_tag="ACHURS_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFR1237441.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            6338..6877
                     /gene="rplE"
                     /locus_tag="ACHURS_001681"
     CDS             6338..6877
                     /gene="rplE"
                     /locus_tag="ACHURS_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFR1237442.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            6892..7197
                     /gene="rpsN"
                     /locus_tag="ACHURS_001682"
     CDS             6892..7197
                     /gene="rpsN"
                     /locus_tag="ACHURS_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFR1237443.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            7231..7623
                     /gene="rpsH"
                     /locus_tag="ACHURS_001683"
     CDS             7231..7623
                     /gene="rpsH"
                     /locus_tag="ACHURS_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFR1237444.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            7636..8169
                     /gene="rplF"
                     /locus_tag="ACHURS_001684"
     CDS             7636..8169
                     /gene="rplF"
                     /locus_tag="ACHURS_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFR1237445.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGVDKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            8179..8532
                     /gene="rplR"
                     /locus_tag="ACHURS_001685"
     CDS             8179..8532
                     /gene="rplR"
                     /locus_tag="ACHURS_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFR1237446.1"
                     /translation="MDKKFARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            8547..9050
                     /gene="rpsE"
                     /locus_tag="ACHURS_001686"
     CDS             8547..9050
                     /gene="rpsE"
                     /locus_tag="ACHURS_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFR1237447.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            9054..9233
                     /gene="rpmD"
                     /locus_tag="ACHURS_001687"
     CDS             9054..9233
                     /gene="rpmD"
                     /locus_tag="ACHURS_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFR1237448.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            9237..9671
                     /gene="rplO"
                     /locus_tag="ACHURS_001688"
     CDS             9237..9671
                     /gene="rplO"
                     /locus_tag="ACHURS_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFR1237449.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            9679..11010
                     /gene="secY"
                     /locus_tag="ACHURS_001689"
     CDS             9679..11010
                     /gene="secY"
                     /locus_tag="ACHURS_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFR1237450.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DATVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFVGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            11042..11158
                     /gene="rpmJ"
                     /locus_tag="ACHURS_001690"
     CDS             11042..11158
                     /gene="rpmJ"
                     /locus_tag="ACHURS_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFR1237451.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            11305..11661
                     /gene="rpsM"
                     /locus_tag="ACHURS_001691"
     CDS             11305..11661
                     /gene="rpsM"
                     /locus_tag="ACHURS_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFR1237452.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            11678..12067
                     /gene="rpsK"
                     /locus_tag="ACHURS_001692"
     CDS             11678..12067
                     /gene="rpsK"
                     /locus_tag="ACHURS_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFR1237453.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            12101..12721
                     /gene="rpsD"
                     /locus_tag="ACHURS_001693"
     CDS             12101..12721
                     /gene="rpsD"
                     /locus_tag="ACHURS_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFR1237454.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            12747..13736
                     /gene="rpoA"
                     /locus_tag="ACHURS_001694"
     CDS             12747..13736
                     /gene="rpoA"
                     /locus_tag="ACHURS_001694"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFR1237455.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            13777..14160
                     /gene="rplQ"
                     /locus_tag="ACHURS_001695"
     CDS             13777..14160
                     /gene="rplQ"
                     /locus_tag="ACHURS_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFR1237456.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            14267..14635
                     /locus_tag="ACHURS_001696"
     CDS             14267..14635
                     /locus_tag="ACHURS_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFR1237457.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            14646..15071
                     /gene="zntR"
                     /locus_tag="ACHURS_001697"
     CDS             14646..15071
                     /gene="zntR"
                     /locus_tag="ACHURS_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFR1237458.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            15127..15345
                     /gene="arfA"
                     /locus_tag="ACHURS_001698"
     CDS             15127..15345
                     /gene="arfA"
                     /locus_tag="ACHURS_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFR1237459.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(15342..15752)
                     /gene="mscL"
                     /locus_tag="ACHURS_001699"
     CDS             complement(15342..15752)
                     /gene="mscL"
                     /locus_tag="ACHURS_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFR1237460.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(15882..17258)
                     /gene="trkA"
                     /locus_tag="ACHURS_001700"
     CDS             complement(15882..17258)
                     /gene="trkA"
                     /locus_tag="ACHURS_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFR1237461.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(17280..18569)
                     /gene="rsmB"
                     /locus_tag="ACHURS_001701"
     CDS             complement(17280..18569)
                     /gene="rsmB"
                     /locus_tag="ACHURS_001701"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFR1237462.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQHQQEELLAEFNTSDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVHDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(18615..19562)
                     /gene="fmt"
                     /locus_tag="ACHURS_001702"
     CDS             complement(18615..19562)
                     /gene="fmt"
                     /locus_tag="ACHURS_001702"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFR1237463.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQESQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLA"
     gene            complement(19577..20086)
                     /gene="def"
                     /locus_tag="ACHURS_001703"
     CDS             complement(19577..20086)
                     /gene="def"
                     /locus_tag="ACHURS_001703"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFR1237464.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            20216..21340
                     /gene="dprA"
                     /locus_tag="ACHURS_001704"
     CDS             20216..21340
                     /gene="dprA"
                     /locus_tag="ACHURS_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFR1237465.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLVTTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQRREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            21312..21785
                     /gene="smg"
                     /locus_tag="ACHURS_001705"
     CDS             21312..21785
                     /gene="smg"
                     /locus_tag="ACHURS_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFR1237466.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            21814..22356
                     /locus_tag="ACHURS_001706"
     CDS             21814..22356
                     /locus_tag="ACHURS_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFR1237467.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RLLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            22361..22933
                     /gene="tsaC"
                     /locus_tag="ACHURS_001707"
     CDS             22361..22933
                     /gene="tsaC"
                     /locus_tag="ACHURS_001707"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFR1237468.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            22938..23756
                     /gene="aroE"
                     /locus_tag="ACHURS_001708"
     CDS             22938..23756
                     /gene="aroE"
                     /locus_tag="ACHURS_001708"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFR1237469.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            23753..24010
                     /locus_tag="ACHURS_001709"
     CDS             23753..24010
                     /locus_tag="ACHURS_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFR1237470.1"
                     /translation="MNQAIQFPDREEWDENKRCVCFPALVNGMQLTCAISGESLAYRF
                     TGGTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(23986..24540)
                     /locus_tag="ACHURS_001710"
     CDS             complement(23986..24540)
                     /locus_tag="ACHURS_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFR1237471.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
     gene            25013..>25284
                     /locus_tag="ACHURS_001711"
     rRNA            25013..>25284
                     /locus_tag="ACHURS_001711"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         6442 a         5992 c         6617 g         6233 t
ORIGIN      
        1 gcttctttaa tgggcgcttt ggatagtaaa agcagaagtc actgtagagg ctgaaacgcc
       61 cgcttttaaa caatcaacac aacactattt cttcaaaaaa cagccactcc ccgtcgtcga
      121 atccgtcgag gaggaagatg acccagacgt cgcggttgag aatgctccct cttcttctga
      181 agacgaagaa aatactgtaa aagaaagcga cgagaaagcc ggtttaagag agcgtgtcaa
      241 aaatgcgctg aacgaacttg agagataacc cgaaggctgt ttactttact agcaatacgc
      301 ttgcgttcgg tggttaagta tgtataatgc gcgggcttgt cgtagttgac agcaggttca
      361 atctgaacct cagttagccg aatttggcta cctaacaatg ctcccaatcg gggagctacg
      421 taagaacggt tacactctcc catcaatcgt aatgggtctg aggagtaatc attttcgttt
      481 ataaaataat tggagctctg gtctcatgca gaaccaaaga atccgtatcc gcctgaaagc
      541 gtttgatcat cgtctgatcg atcaagcaac cgcggaaatc gtcgagactg ccaagcgcac
      601 tggtgcgcag gtccgtggtc cgatcccgct gccgacacgc aaagagcgct tcactgttct
      661 gatctccccg cacgtcaaca aagacgcgcg cgatcagtac gaaatccgta ctcacttgcg
      721 tctggttgac atcgttgagc caaccgagaa aaccgttgat gctctgatgc gtctggatct
      781 ggctgccggt gtagacgtgc agatcagcct gggttaatca ggtcattgag cgattgagag
      841 gttgaaacaa tgattggttt agtcggtaaa aaagtgggta tgacccgtat cttcacagaa
      901 gacggcgttt ctatcccagt aaccgtaatc gaagttgaag caaaccgcgt tactcaggtt
      961 aaagacctgg ctaacgatgg ctaccgtgct attcaggtga ccaccggtgc taaaaaagct
     1021 aaccgtgtga ccaagcctga agctggccac ttcgctaaag ctggcgtaga agctggccgt
     1081 ggtctgtggg aattccgcct ggctgaaggc gaagagttca ctgtaggtca gagcattagc
     1141 gttgaactgt ttgctgaggt taaaaaagtt gacgtaactg gcacctctaa aggtaaaggt
     1201 ttcgcaggta ccgttaagcg ctggaacttc cgtacccagg acgctactca cggtaactcc
     1261 ttgtctcacc gcgttccggg ttctatcggt cagaaccaga ctccgggcaa agtgttcaaa
     1321 ggcaagaaaa tggcaggtca gatgggtaac gaacgtgtaa ccgttcagag ccttgacgta
     1381 gtacgcgttg acgctgagcg caacctgctg ctggttaaag gtgctgtccc gggtgcaacc
     1441 ggtagcgacc tgatcgttaa accagctgtg aaggcgtaag gagatagcaa tggaattagt
     1501 attgaaagac gcgcagagcg cgctgactgt ttccgaaact accttcggtc gtgatttcaa
     1561 cgaagcgctg gttcaccagg ttgttgttgc ttatgcagct ggtgctcgtc agggtactcg
     1621 tgctcagaag actcgtgctg aagtaactgg ttccggtaaa aaaccgtggc gccagaaagg
     1681 caccggccgt gcgcgttctg gttctatcaa gagcccgatc tggcgttctg gtggcgtgac
     1741 ctttgctgct cgtccgcagg accacagtca aaaagttaac aagaagatgt accgcggcgc
     1801 gctgaaaagc atcctgtccg aactggtacg tcaggatcgt ctgatcgttg tcgagaagtt
     1861 ctctgtagaa gcgccgaaaa ctaagctgct ggcacagaaa ctgaaagaca tggctctgga
     1921 agatgtgctg atcatcaccg gtgagctgga cgaaaacctg ttcctggctg cgcgcaacct
     1981 gcacaaggtt gacgtacgcg atgcaactgg tatcgacccg gttagcctga tcgccttcga
     2041 caaagtcgta atgactgctg atgctgttaa gcaagttgag gagatgctgg catgattcgt
     2101 gaagaacgtc tgctgaaggt gctgcgtgca ccgcacgttt ctgaaaaagc gtctactgcg
     2161 atggaaaaat ccaacaccat cgtactcaaa gttgctaaag acgcgaccaa agcagaaatc
     2221 aaagctgctg tgcagaaact gtttgaagtc gaagtcgaag tcgttaacac cctggtagtt
     2281 aaagggaaag ttaaacgtca cggacagcgt atcggtcgtc gtagcgactg gaaaaaagct
     2341 tacgtcaccc tgaaagaagg ccagaatctg gacttcgttg gcggcgctga gtaagtcgga
     2401 ggagtaatac aatggcagtt gttaaatgta aaccgacatc tccgggtcgt cgccacgtag
     2461 ttaaagtggt taaccctgag ctgcacaagg gcaaaccttt tgctccgttg ctggaaaaaa
     2521 acagcaaatc cggtggtcgt aacaacaatg gccgtatcac cactcgtcat atcggtggtg
     2581 gccacaagca ggcttaccgt attgttgact tcaaacgcaa caaagacggt atcccggcag
     2641 ttgttgaacg tcttgagtac gatccgaacc gttccgcgaa catcgcgctg gttctgtaca
     2701 aagacggtga acgccgttac atcctggccc ctaaaggcct gaaagctggc gaccagattc
     2761 agtctggcgt tgatgctgca atcaaaccag gtaacaccct gccgatgcgc aacatcccgg
     2821 ttggttctac tgttcataac gtagaaatga aaccaggtaa aggcggtcag ctggcacgtt
     2881 ccgctggtac ttacgttcag atcgttgctc gtgatggtgc ttatgtcacc ctgcgtctgc
     2941 gttctggtga aatgcgtaaa gtagaagcag actgccgtgc aactctgggc gaagttggca
     3001 atgctgagca tatgctgcgc gttctgggta aagcaggtgc tgcacgctgg cgtggtgttc
     3061 gtccgaccgt tcgcggtacc gcgatgaacc cggtagacca cccacatggt ggtggtgaag
     3121 gtcgtaactt tggtaagcac ccggtaactc cgtggggcgt tcagaccaaa ggtaagaaga
     3181 cccgcagcaa caagcgtact gataaattca tcgtacgtcg ccgtagcaaa taattttaga
     3241 ggataagcca tgccacgttc tctcaagaaa ggtcctttta ttgacctgca cttgctgaag
     3301 aaggtagaga aagcggtgga aagcggagac aagaagcccc tgcgcacttg gtcccgtcgt
     3361 tcaacgatct ttcctaacat gatcggtttg accatcgctg tccataatgg tcgtcagcac
     3421 gttccggtat ttgtgaccga cgaaatggtc ggtcacaaac tgggtgaatt cgcaccgact
     3481 cgtacttatc gcggccacgc tgctgataaa aaagcgaaga agaaataagg taggaggaag
     3541 agatggaaac tatcgctaaa catcgccatg ctcgttcttc tgctcagaag gttcgccttg
     3601 ttgctgacct gattcgcggt aagaaagtgt cgcaggctct ggatattttg acctacacca
     3661 acaagaaagc ggctgtactg gtcaagaaag ttctggaatc tgccattgct aacgctgaac
     3721 acaacgatgg cgctgacatt gacgatctga aagttacgaa aattttcgta gacgaaggcc
     3781 cgagcatgaa gcgcattatg ccgcgtgcaa aaggtcgtgc agatcgcatc ctgaagcgca
     3841 ccagccacat cactgtggtt gtgtccgatc gctgagactc tggagactag caatgggtca
     3901 gaaagtacat cctaatggta ttcgcttggg tattgtaaag ccatggaact ctacctggtt
     3961 tgcgaacacc aaagaattcg ctgacaacct ggacagcgat tttaaagtac gtcagtacct
     4021 gactaaggaa ctggctaaag cgtccgtatc tcgtatcgtt atcgagcgtc cggctaagag
     4081 catccgtgta accattcaca ctgctcgccc gggtatcgtt atcggtaaaa aaggtgaaga
     4141 cgtagaaaaa ctgcgtaagg tcgtagcgga tatcgctggc gttcctgcac agatcaacat
     4201 cgccgaagtt cgtaagcctg aactggacgc aaaactggtt gctgacagca tcacttctca
     4261 gctggaacgt cgcgttatgt tccgtcgtgc tatgaagcgt gctgtacaga acgcaatgcg
     4321 tctgggcgct aaaggtatta aagttgaagt tagcggccgt ctgggcggcg cggaaatcgc
     4381 acgtaccgaa tggtaccgcg aaggtcgcgt accgctgcac actctgcgtg ctgacatcga
     4441 ctacaacacc tctgaagcgc acaccactta cggtgtaatc ggcgttaaag tgtggatctt
     4501 caaaggcgag atcctgggtg gtatggctgc tgttgaacaa ccggaaaaac cggctgctca
     4561 gcctaaaaag cagcagcgta aaggccgtaa ataaggagcg tcgctgatgt tacaaccaaa
     4621 gcgtacaaaa ttccgtaaaa tgcacaaagg ccgtaaccgc ggtctggcgc agggtacgga
     4681 tgttagcttc ggcagcttcg gtctgaaagc tgttggccgt ggtcgtctga ctgcccgtca
     4741 gatcgaagca gcacgtcgtg ctatgacccg tgcagttaag cgtcaaggta agatctggat
     4801 ccgtgtgttc ccggacaaac cgatcactga aaagccgctg gcagtgcgta tgggtaaagg
     4861 taaaggtaac gtggagtatt gggttgcctt gattcagccg ggtaaagtcc tgtatgaaat
     4921 ggacggtgtt ccggaagagc tggcccgtga agcattcaag ctggcagcag cgaaactgcc
     4981 gattaaaacc acctttgtaa ctaagacggt gatgtaatga aagcaaaaga gctgcgtgag
     5041 aagagcgttg aagagctgaa caccgagctg ctgaacctgc tgcgtgagca gttcaacctg
     5101 cgtatgcagg ctgcaagtgg ccagctgcaa cagtctcacc tgttgaagca agtgcgtcgc
     5161 gatgtcgcac gcgttaagac tttactgaac gagaaggcgg gtgcgtaatg accgataaaa
     5221 tccgtactct gcaaggtcgc gttgttagcg acaaaatgga gaaatccatt gttgttgcta
     5281 tcgaacgttt tgtgaaacac ccgatctacg gtaaattcat caagcgtacg accaaactgc
     5341 acgtacatga cgagaacaac gaatgcggta tcggtgacgt ggttgaaatc cgcgaatgcc
     5401 gtccgctgtc caagactaaa tcctggacgc tggttcgcgt tgtagagaaa gcggttctgt
     5461 aatacagtac actctctcga tacgaataaa cggctcagaa atgagccgtt tattttttct
     5521 acccatatcc ttgaagcggt gttataatgc cgcgccctcg atatggggat ttttaacgac
     5581 ctgattttcg ggtctcagta gtagttgaca ttagcggagc actaaaatga tccaagaaca
     5641 gactatgctg aacgtcgccg acaactccgg tgcacgtcgc gtaatgtgta tcaaggttct
     5701 gggtggctcg caccgtcgct acgcaggcgt aggcgacatc atcaagatca ccatcaaaga
     5761 agcaattccg cgtggtaagg tcaaaaaagg tgatgtgctg aaggcggtag tggtgcgcac
     5821 caagaagggt gttcgtcgcc cggacggttc tgtcattcgc ttcgatggta atgcttgtgt
     5881 tcttctgaac aacaacagcg agcagcctat cggtacgcgt atttttgggc cggtaactcg
     5941 tgagcttcgt agtgagaagt tcatgaaaat tatctctctg gcaccagaag tactctaagg
     6001 agcgaatcat ggcagcgaaa atccgtcgtg atgacgaagt tatcgtgtta accggtaaag
     6061 ataaaggtaa acgcggtaaa gttaagaatg tcctgtcttc cggcaaggtc attgttgaag
     6121 gtatcaacct ggttaagaaa catcagaagc cggttccggc cctgaaccaa ccgggtggca
     6181 tcgttgaaaa agaagccgct attcaggttt ccaacgtagc aatcttcaat gcggcaaccg
     6241 gcaaggctga ccgtgtaggc tttagattcg aagacggcaa aaaagtccgt ttcttcaagt
     6301 ctaacagcga aactatcaag taatttggag tagtacgatg gcgaaactgc atgattacta
     6361 caaagacgaa gtagttaaaa aactcatgac tgagtttaac tacaattctg tcatgcaagt
     6421 ccctcgggtc gagaagatca ccctgaacat gggtgttggt gaagcgatcg ctgacaaaaa
     6481 actgctggat aacgcagcag cagacctggc agcaatctcc ggtcaaaaac cgctgatcac
     6541 caaagcacgc aaatctgttg caggcttcaa aatccgtcag ggctatccga tcggctgtaa
     6601 agtaactctg cgtggcgaac gcatgtggga gttctttgag cgcctgatca ctattgctgt
     6661 acctcgtatt cgtgacttcc gtggcctgtc cgctaagtct ttcgacggtc gtggtaacta
     6721 cagcatgggt gtccgtgagc agatcatctt cccagaaatc gactacgata aagtcgaccg
     6781 cgttcgtggt ttggatatta ccattaccac tactgcgaaa tctgacgaag aaggccgcgc
     6841 tctgctggct gcctttgact tcccgttccg caagtaaggt agggttacta aatggctaag
     6901 caatcaatga aagcacgcga agtaaaacgc gtagctttag ctgataaata cttcgcgaaa
     6961 cgcgctgaac tgaaagcgat catctctgat gtgaacgctt ccgacgaaga tcgttggaac
     7021 gctgttctca agctgcagac tctgccgcgt gattccagcc cgtctcgtca gcgtaaccgc
     7081 tgccgtcaaa caggtcgccc gcatggtttc ctgcggaagt tcgggttgag ccgtattaag
     7141 gtccgtgaag ccgctatgcg cggtgaaatc ccgggtctga aaaaggctag ctggtaattg
     7201 tcaccaattg aatcacggga gtaaagacag atgagcatgc aagatccgat cgcggatatg
     7261 ctgacccgta tccgtaacgg tcaggccgcg aacaaagctg cggtcaccat gccttcctcc
     7321 aagctgaaag tggcaatcgc caacgtgctg aaggaagaag gttttattga agattttaaa
     7381 gttgaaggcg acaccaagcc tgaactggaa cttactctga agtatttcca gggcaaagct
     7441 gttgtagaaa gcattcagcg tgtcagccgc ccaggtctgc gcatctataa acgtaaagat
     7501 gagctgccga aagttatggc gggtctgggt atcgcagttg tttctacctc taaaggtgtt
     7561 atgactgatc gtgcagcgcg ccaggctggt cttggtggcg aaattatctg ctacgtagcc
     7621 taatcggagg aaaaaatgtc tcgtgttgct aaagcaccgg tcgttgttcc tgccggcgtt
     7681 gacgtaaaaa tcaacggtca ggttattacg atcaaaggta aaaacggcga gctgactcgt
     7741 actctcaacg atgctgttga agttaaacat gcagataata ccctgacctt cggtccgcgt
     7801 gatggttacg cagacggttg ggcacaggct ggtaccgcgc gtgccctgct gaactcaatg
     7861 gttatcggtg ttaccgaagg cttcactaag aagctgcagc tggttggtgt aggttaccgt
     7921 gcagcggtta aaggcaatgt gattaacctg tctctgggtt tctctcatcc tgttgaccat
     7981 cagctgcctg cgggtatcac tgctgaatgt ccgactcaga ctgaaatcgt gctgaaaggc
     8041 gttgataagc aggtgatcgg ccaggttgca gcggatctgc gcgcctaccg tcgtcctgag
     8101 ccttataaag gcaagggtgt tcgttacgcc gacgaagtcg tgcgtaccaa agaggctaag
     8161 aagaagtaag gtaacactat ggataagaaa tttgctcgta tccgtcgtgc gacccgcgca
     8221 cgccgcaagc tccaggagct gggcgcaact cgcctggtgg tacatcgtac cccgcgtcac
     8281 atttacgcac aggtaattgc accgaacggt tctgaagttc tggtagctgc ttctactgta
     8341 gaaaaagcta tcgctgaaca actgaagtac accggtaaca aagacgcggc tgcagctgtg
     8401 ggtaaagctg tcgctgaacg cgctctggaa aaaggcatca aagatgtatc ctttgaccgt
     8461 tccgggttcc aatatcatgg tcgtgtccag gcactggcag atgctgcccg tgaagctggc
     8521 cttcagttct aaggtagagg tgtaagatgg ctcacatcga aaaacaagct ggcgaactgc
     8581 aggaaaagct gatcgcggta aaccgcgtat ctaaaaccgt taaaggtggt cgtattttct
     8641 ccttcacagc tctgactgta gttggcgatg gtaacggtcg cgttggtttt ggttacggta
     8701 aagcgcgtga agttccagca gcgatccaga aagcgatgga aaaagcccgt cgcaatatga
     8761 ttaacgtcgc gctgaataac ggcactctgc aacaccctgt taaaggtgtt cacacgggtt
     8821 ctcgcgtatt catgcagccg gcttccgaag gtaccggtat catcgccggt ggtgcaatgc
     8881 gcgccgttct ggaagtcgct ggggttcata acgttctggc taaagcatat ggttccacca
     8941 acccgatcaa cgtggttcgt gcaactattg atggcctgga aaatatgaat tctccagaaa
     9001 tggtcgctgc caagcgtggt aaatccgttg aagaaattct ggggaaataa accatggcaa
     9061 agactattaa aattactcaa acccgcagtg caatcggtcg tctgccgaaa cacaaggcaa
     9121 cgctgcttgg cctgggtctg cgtcgtattg gtcacaccgt agagcgcgag gatactcctg
     9181 ctattcgcgg tatgatcaac gcggtttcct tcatggttaa agttgaggag taagagatgc
     9241 gtttaaatac tctgtctccg gccgaaggct ccaaaaaggc gggtaaacgc ctgggtcgtg
     9301 gtatcggttc tggcctcggt aaaaccggtg gtcgtggtca caaaggtcag aagtctcgtt
     9361 ctggcggtgg cgtacgtcgc ggtttcgagg gtggtcagat gcctctgtac cgtcgtctgc
     9421 cgaaattcgg cttcacttct cgtaaagcag cgattacagc cgaagttcgt ctgtctgacc
     9481 tggctaaagt agaaggcggt gttgtagacc tgaacacgct gaaagcggct aacattatcg
     9541 gtatccagat cgagttcgcg aaagtgatcc tggctggcga agtaacgact ccggtaactg
     9601 ttcgtggcct gcgtgttact aaaggcgctc gtgctgctat cgaagctgct ggcggtaaaa
     9661 tcgaggaata agtagcagat ggctaaacaa ccgggattag attttcaaag tgccaaaggt
     9721 ggcttaggcg agctgaaacg cagactgctg tttgttatcg gtgcgctgat tgtgttccgt
     9781 attggctctt ttattccgat ccctggtatt gatgccactg tacttgccaa actgcttgag
     9841 caacagcgag gcaccatcat tgagatgttt aacatgttct ctggtggtgc tctcagccgt
     9901 gcttctatct ttgctctggg gatcatgccg tatatttcgg cgtcgatcat tatccagctg
     9961 ctgacggtgg ttcacccaac gttggcggaa attaagaaag aaggggagtc tggtcgtcgt
    10021 aagatcagcc agtacacccg ctacggtact ctggtgctgg caatattcca gtcgatcggt
    10081 attgctactg gtctgccgaa tatgcctggt atgcaaggcc tggtgattaa cccgggcttt
    10141 gcattctact tcaccgctgt tgtaagtctg gtcacaggaa cgatgttcct gatgtggttg
    10201 ggcgaacaga ttactgaacg aggtatcggc aacggtattt caatcattat cttcgtcggt
    10261 attgtcgcgg gactcccgcc agccattgcc catactatcg agcaagcgcg tcaaggcgac
    10321 ctgcacttcc tcgtgttgct gttggttgca gtattagtat ttgcagtgac gttctttgtt
    10381 gtatttgttg agcgtggtca acgccgcatt gtggtaaact acgcgaaacg tcagcaaggt
    10441 cgtcgtgttt atgctgcaca gagcacacat ttaccgctga aagtgaatat ggcgggggta
    10501 atcccggcaa tcttcgcttc cagtattatt ctgttcccgg cgaccatcgc gtcatggttc
    10561 gggggcggta ctggttggaa ctggctgaca acaatttcgc tgtatttgca gcctgggcaa
    10621 ccgctttatg tgttactcta tgcgtctgca atcatcttct tctgtttctt ctacacggcg
    10681 ttggttttca acccgcgtga aacagcagat aacctgaaga agtccggtgc atttgtacca
    10741 ggaattcgtc cgggagagca aacggcgaag tatatcgata aagtaatgac ccgcctgacc
    10801 ctggttggtg cgctgtatat tacctttatc tgcctgatcc cggagttcat gcgtgatgca
    10861 atgaaagtac cgttctactt cggtgggacc tcactgctta tcgttgttgt cgtgattatg
    10921 gactttatgg ctcaagtgca aactctgatg atgtccagtc agtatgagtc tgcattgaag
    10981 aaggcgaacc tgaaaggcta cggccgataa ttggtcgccc gagaagttac ggagagtaaa
    11041 aatgaaagtt cgtgcttccg tcaagaaatt atgccgtaac tgcaaaatcg ttaagcgtga
    11101 tggtgtcatc cgtgtgattt gcagtgccga gccgaagcat aaacagcgcc aaggctgatt
    11161 ttttcgcata tttttcttgc aaagttgggt tgagctggct agattagcca gccaatcttt
    11221 tgtatgtctg tgcgtttcca tttgagtatc ctgaaaacgg gcttttcagc atggaacgta
    11281 catattaaat agtaggagtg catagtggcc cgtatagcag gcattaacat tcctgatcat
    11341 aagcatgccg taatcgcatt aacttcgatt tatggcgtcg gcaagacccg ttctaaagcc
    11401 atcctggctg cagcgggtat cgctgaagat gttaagatca gtgagctgtc tgaaggacaa
    11461 atcgacacgc tgcgtgacga agttgccaaa tttgtcgttg aaggtgatct gcgccgtgaa
    11521 atcagcatga gcatcaagcg cctgatggat cttggttgct atcgcggttt gcgtcatcgt
    11581 cgtggtctcc cggttcgcgg tcagcgtacc aagaccaacg cacgtacccg taagggtccg
    11641 cgcaaaccga tcaagaaata atcggggtga ttgaataatg gcaaaggcac caattcgtgc
    11701 acgtaaacgt gtaagaaaac aagtctctga cggcgtggct catatccatg cttctttcaa
    11761 caacaccatc gtgactatca ctgatcgtca gggtaacgcg ttgggttggg caacagccgg
    11821 tggttccggt ttccgtggtt ctcgcaaatc cactccgttt gcagctcagg ttgcagcaga
    11881 gcgttgcgct gacgccgtga aagaatacgg catcaagaat ctggaagtta tggttaaagg
    11941 tccgggtcca ggccgcgaat ctactattcg tgctctgaac gccgcaggtt tccgcatcac
    12001 taacattact gatgtgactc cgatccctca taacggttgt cgtccgccga aaaaacgtcg
    12061 cgtataacgc ctcgttttcc aggtttgttg gagaaagaaa atggcaagat atttgggtcc
    12121 taagctcaag ctgagccgtc gtgagggcac cgacttattc cttaagtctg gcgttcgcgc
    12181 gatcgatacc aagtgtaaaa ttgaacaagc tcctggccag cacggtgcgc gtaaaccgcg
    12241 tctgtctgac tatggtgtgc agttgcgtga aaagcaaaaa gttcgccgta tctatggtgt
    12301 gctggagcgt cagttccgta actactacaa agaagcagca cgtctgaaag gcaacaccgg
    12361 tgaaaacctg ttggctctgc tggaaggtcg tctggacaac gttgtatacc gtatgggctt
    12421 cggtgccact cgtgcagaag cacgtcagct ggttagccat aaagcaatta tggtaaacgg
    12481 tcgtgttgtt aacatcgctt cttatcaggt tagtccgaat gacgttgtaa gcattcgtga
    12541 gaaagcgaag aagcagtctc gcgtgaaagc cgctctggag ctggctgagc agcgtgaaaa
    12601 gccaacctgg ctggaagttg atgctggcaa gatggaaggt acgtttaagc gtaagccgga
    12661 gcgttctgat ctgtctgcgg acattaacga acacctgatc gtcgagcttt actccaagta
    12721 aagcttagta ccaaagagag gacacaatgc agggttctgt gacagagttt ctaaaaccgc
    12781 gcctggttga tatcgagcaa gtgagttcga cgcacgccaa ggtgaccctt gagcctttag
    12841 agcgtggctt tggccatact ctgggtaacg cactgcgccg tattctgctc tcatcgatgc
    12901 cgggttgcgc ggtgaccgag gttgagattg atggtgtact acatgagtac agcaccaaag
    12961 aaggcgttca ggaagatatc ctggaaatcc tgctcaacct gaaagggctg gcggtgagag
    13021 ttcagggcaa agatgaagtt attcttacct tgaataaatc tggcattggc cctgtgactg
    13081 cagccgatat cacccacgac ggtgatgtcg aaatcgtcaa gccgcagcac gtgatctgcc
    13141 acctgaccga tgagaacgcg tctattagca tgcgtatcaa agttcagcgc ggtcgtggtt
    13201 atgtgccggc ttctacccga attcattcgg aagaagatga gcgcccaatc ggccgtctgc
    13261 tggtcgacgc atgctacagc cctgtggagc gtattgccta caatgttgaa gcagcgcgtg
    13321 tagaacagcg taccgacctg gacaagctgg tcatcgaaat ggaaaccaac ggcacaatcg
    13381 atcctgaaga ggcgattcgt cgtgcggcaa ccattctggc tgaacaactg gaagctttcg
    13441 ttgacttacg tgatgtacgt cagcctgaag tgaaagaaga gaaaccagag ttcgatccga
    13501 tcctgctgcg ccctgttgac gatctggaat tgactgtccg ctctgctaac tgccttaaag
    13561 cagaagctat ccactatatc ggtgatctgg tacagcgtac cgaggttgag ctccttaaaa
    13621 cgcctaacct tggtaaaaaa tctcttactg agattaaaga cgtgctggct tcccgtggac
    13681 tgtctctggg catgcgcctg gaaaactggc caccggcaag catcgctgac gagtaaccgg
    13741 atcacaggtt aaggttttac tgagaaggat aaggtcatgc gccatcgtaa gagtggtcgt
    13801 caactgaacc gcaacagcag ccatcgccag gctatgttcc gcaatatggc aggttcactg
    13861 gttcgtcatg aaatcatcaa gacgactctg cctaaagcga aagagctgcg ccgcgtagtt
    13921 gagccgctga ttactcttgc caagactgat agcgttgcta atcgtcgtct ggcattcgcc
    13981 cgtactcgtg ataacgagat cgtggcaaaa ctgtttaacg aactgggccc gcgtttcgcg
    14041 agccgtgccg gtggttacac tcgtattctg aagtgtggct tccgtgcagg cgacaacgcg
    14101 ccgatggctt acatcgagct ggttgatcgt tcagagaaag cagaagctgc tgcagagtaa
    14161 tctgaagcaa tgtaaaaaaa cccgccccgg cgggtttttt tatacccgta gtatccccac
    14221 ttatctacaa tagctgtact ctttttgttc atcccctgga gtatttatgt ggttacttga
    14281 ccagtgggca gagcgccata tagcagaagc gcaagcgaaa ggtgagtttg ataacctggc
    14341 aggtagcggc gaaccattga tactggatga tgattctcac gtgccaccgg aattacgtgc
    14401 ggggtatcgc ttgctgaaga atgccggttg cttaccgcca gaacttgagc aacggagaga
    14461 agcaattcag cttctggata ttctcaaagg tatccgtcac gatgatccgc aatatcaaga
    14521 ggttagccgt cgattgtcat tactggaatt gaagctgcga caagctggat tgagtaccga
    14581 ttttttacgc ggcgattatg ctgacaagtt gttggacaaa atcaacgata actagtggag
    14641 tatgtatgta tcgcattggt gagctggcaa aaatggcgga agtaacacct gacacgattc
    14701 gttattacga aaaacagcag atgatggagc atgaagtgcg tactgaaggc gggtttcgcc
    14761 tgtataccga aagcgatctc cagcgattga aatttatccg ccatgccaga caactaggtt
    14821 tcagtctgga gtcgatccgc gagttgctgt cgatccgcat cgatcctgaa caccatacct
    14881 gtcaggagtc aaaaggcatt gtgcaggaaa gattgcagga agtcgaagca cggatagccg
    14941 agttgcagag tatgcagcgt tccttgcaac gccttaacga tgcctgttgt gggaccgccc
    15001 atagcagtgt ttattgctcg attcttgaag ctcttgaaca aggggcgagt ggcgttaaga
    15061 gtggctgttg attttttgca ctgacaggat tacactcgcg ccgttaaata accaactgga
    15121 gtttttatga gccgatatca gcatactaaa gggcagataa aggataatgc gatagaagca
    15181 ttactacatg atcccttatt ccgacagcga gtagagaaaa ataagaaagg gaaaggcagt
    15241 tacatgcgaa aaggaaaaca tggcaatcgg gggaactggg aggccagtgg caagaaagta
    15301 aatcactttt ttaccactgg tcttctgctt tcaggcgctt gttaagagcg gttattctgc
    15361 tctttcagca aatcacgaat ttctgccagt aatacttctt ctttagttgg tgcaggtgcg
    15421 gcagctggtt cttctttttt ccgattcagt ttgttgatta gcttaatcgc cataaagatg
    15481 gcaaaggcca caatcagaaa atcaaagacg ttttgaatga agacaccgta atgcatcaca
    15541 acagcaggga tatccccctg cgcatcgcgt agcgtgacag caaactgttt aaagtcgatc
    15601 ccgccaatta ataagcccag aggtggcatg atgatatcgg caaccagtga agatacaatc
    15661 ttcccgaatg ccgcaccgat aatgacaccc accgccaaat ccaccacgtt cccgcgcatc
    15721 gcaaattcgc gaaattcttt aataatgctc atgttattct ccctatgaag ccgacaacca
    15781 taagtctaac aaatgttaag ccattttcct gccaggaatg aatttaatta atccttgata
    15841 atcaaaggga aataataagg cgtcattaga cgccttatta attacaagaa gaaagggctt
    15901 ggctggaaga gtctttcgac gtcggtaata aactttttat ctgtgaggaa cataattacg
    15961 tgatcgcctt gctcaatgcg cagattgtca ttggcaatca tcacgtcgtt tccacgtacc
    16021 accgctccaa taatcgttcc tggcggtagc ttgatttcgt caatgactct gccgacaacg
    16081 cgtgaggtgc tttcatcacc gtgagcaacg gcttcaatag cttctgctac gccgcggcgc
    16141 aatgaggaaa caccaacaat atctgctttt cgcacatggc taagcaacgc agaaatagtt
    16201 gcttgttgtg gtgaaattgc aatatcgatc acgctcccct gaaccagatc cacataagcg
    16261 cgacgctgga tcaataccat cacctttttc gcacccatac gtttggcaag catggcggac
    16321 atgatattgg cctcgtcatc gttggtgacg gcaataaaca gatcaacttg atcgatatgt
    16381 tcttcggcca gcagttcttg atccgacgca tcaccaaaaa agacgatcgt attctgcaac
    16441 ttttccgcca gttcggcagc gcgctgctga ttacgttcga tgagtttaac gctgtaatct
    16501 ttttccagac gacgcgccag ccctgcaccg atattaccgc caccaaccag catgatccgc
    16561 ttatacggtt tttccagtcg ctgtaattca ctcatcaccg cgcggatatg ctgtgaagcg
    16621 gcaataaaga acacttcatc accagcttca acaatggtcg aaccttgtgg acgaatgggg
    16681 cgatcgtggc ggaaaattgc tgccacacga gtatcgatat gtggcatatg ttcgcgcatg
    16741 gtcgaaagtg cattaccaat cagcgggccg ccataatagg ctttaactac agcgaggctg
    16801 actttaccct cagcgaagtt caccacctgc aatgcgcctg gatactcaat cagtcggtaa
    16861 atattatcga taaccaactg ctctggtgcg atcagatgat caatcggtac agcatctgaa
    16921 tgaaatagct tatcggcatc gcgtacgtag tctggtgagc ggatgcgagc gatgcgatta
    16981 ggggtgttga aaagtgagta ggctacctgg caggcaacca tattggtttc atctgaactg
    17041 gttacagcaa ccagcatatc ggcgtcgtcg gcacctgcct cccgcaatac gcgtggatga
    17101 gagccatgcc cctgcacgac tcgcaggtca aatttatcct gcaaggtccg cagacgctca
    17161 ccgttggtat cgacaacagt aatatcgttg ttctcgccaa ccaggttttc cgccagtgtg
    17221 ccgccaacct ggccggcacc cagaatgata attttcatca gtctcgaccc gttatctcat
    17281 cactttttga ttagcttagc gtaaaagaag ccgtcgccct cttcggcacc aggcagattt
    17341 tgtttacccg gttgctctgg tgttcctgtt tcgcaaagtt cggcatcagc ggtacgttgc
    17401 aaaaaagctt taatctgcag gctattctct tccggtaaca ccgaacaggt ggcatagacc
    17461 agggttccac cggattttaa atgcggccaa atggcgtcga gaatttcaga ctgcaattgc
    17521 gcgagttccg ggatatcgcg atcgcgacgt aaccatttaa tatccggatg gcgacgaatc
    17581 acccccgttg ctgaacaagg cgcatctaat aaaatgcgat caaactgttg ctcgccacac
    17641 cattgggaag ggtaacggcc atcaccttgt ttcacggtcg ccttcatacc aaggcgtttt
    17701 aaattgtcgt gaacgcgaga gaggcgctgt tcgtcaatat caaccgcaac aacctgcgct
    17761 tctggtgcca cctcaaggat atgcgttgtt ttaccgccgg gggcggcaca aagatccaga
    17821 atgtgttcac cgttttgtgg cgcaagccag gtcatgcaac cttgtgctga tgcatcctga
    17881 acggtaaccc atccgtcttc aaaaccaggt agcgcatgaa caggtgcagg tgtttccagc
    17941 cgtacagcat cagggtaatc cgcatgcggg aaacctttca ttcctgcttc atccaacaat
    18001 gcaagccagc tgtcgcggga atgatgcgta cgattaacgc gcagccacat tggcggacgc
    18061 tggttattgg cttcgacgat ggattgccac tgctctggat acgctttttg cagacgcttc
    18121 agcaaccagg aaggatgcag ataacgtgca tcactggtat taaactcggc taataactct
    18181 tcttgctgat gctggaactg gcgtaataca ccgttaatca accctttaag ttgcggacgc
    18241 ttaattgcga tagcgccttc aaccgtttca gccagcgcag catgaggtgg aatgcgggta
    18301 taaagcagtt gatacaaacc aaccataatc aggtaatgca cagtgcgctg tttgccggtc
    18361 atcggacggg ccattaactt attaatcagc cagtctaact gcgaaagcgt acgcagtacg
    18421 ccaaagcaca actcttgaag aagtgctttg tctttatcag aaactttttg ctggagcggt
    18481 ggcagaatgt tgcttaatga ttgcccttgc tcgacgactt gttcaacggc ctgggccgcc
    18541 atgctacgta aattacgttg ttttttcata agtataaaaa tgcccggtaa gaccgggctt
    18601 agaagagtgg actatcaggc cagacggttg cccggaacaa accattcccg acgagaattc
    18661 aggaggtctt gcgcgctcat cgctttctta cccgcaggtt gtaacgagag caggttcagg
    18721 atgccatcac cagtcgcaac ctgaatgcct tgtttgttgg cttcaaggat cgttcctggt
    18781 gcagcgttgg ttgccgtatc aatgaccgac gctttccaga ctttaaccgg ttgtccttca
    18841 atttccagcc agctcattgg ccatgggttg aaagcgcgaa tgcagcgttc aagctgcgct
    18901 gccgaaagcg accagtcaat acgcgcttct tctttactca acttctcggc gtaagtgaca
    18961 agagtttcgt cctgaacttc tggtttcgcc gtgccgtctg ctagttgttt caacgtggtg
    19021 attagccctt gtgggccaag ctctgccagc ttgtcgtaca gcgtaccact ggtatcttct
    19081 gcagtaatcg ggcaggagag cttatagagc atatcaccgg tgtctaaacc gacatccatt
    19141 tgcataatgg tcacaccagt ttctgcatca cccgcccata gtgagcgttg gattggtgca
    19201 gcaccgcgcc agcgtggcag cagtgaacca tgaacgttga tacagccaag acgcggcatc
    19261 tccagcactg ctttaggcag aattaagcca taggcgacga cgaccataac atcagcctgc
    19321 agatcggcga ccagttgctg gctttcttgt ggacgcaggg aaacaggttg aaaaacgggc
    19381 agaccttttt cctcagccag aactttaacc gggctgggca tcagtttttt accgcgtcct
    19441 gccggtcggt ctggctgggt gaacacgcca acgacgttat gaccagaaga caacagcgcg
    19501 tcgagatgac gcgctgcaaa gtcaggtgta cccgcaaaaa taatacgtag tgattctgac
    19561 acgttagttc ttatccttaa gcccgggctt tcagacgatc tagtttttca actttctgac
    19621 gaatacgttg ttgtttcagc ggtgacagat aatccataaa cagtttgccg accaggtgat
    19681 ccatctcatg ctgaatacag atggctaaca gcccctctgc ttccagttca aatggtttac
    19741 cgtcgcggtc aagggcgcga attttaactt tctctgcgcg cggcactaaa gcacgttgtt
    19801 cagggatcga caggcaacct tcttcaatgc ctgtttcgcc gcttttttct aaaagctccg
    19861 gattgattaa caccagccgt tcgtcacggt tttccgaaac atcaataaca atgatacgtt
    19921 gatggatatc aacctgggtt gccgccaggc caataccttc ttctgcgtac atcgtctcga
    19981 acatatcatc gacgatacgc tgtatttctg cattcacttc ttctaccggt ttagcaactt
    20041 tgcgaagccg ctcgtccgga atatgtaaca cttgcaaaac tgacataaat ctccagagat
    20101 gtgttcaggg gttagaaaga ttatttcttc tattctagac aaatccccct ctgattgaca
    20161 gcatcactga ccaatcgcaa agattgctaa ggctgcttat ggcagggaga taaggatggt
    20221 cgatacagaa atttggctgc gtttaatgag tatcagcagc ttgtacggcg atgatatggt
    20281 ccgtatagct cactggctgg caaaacagtc gcaaattgat gcggttggat tgcagcaaac
    20341 agggcttaca ttgcggcagg cacaacgctt tctttcattt ccgcgaaaga gtatcgaaag
    20401 ctcactttgt tggttggagc aacccaacca tcatttaatc cctgcggaca gcgaatttta
    20461 tcctcctcaa cttctggtga cgacagatta ccccggcgca ctgtttgttg aaggagaact
    20521 gcacgcgctg cattcatttc agcttgccgt agtggggagt cgggcgcatt catggtatgg
    20581 cgagcgatgg ggacggttat tttgcgaaac tctggcgacg cgtggagtga caattacgag
    20641 tggactggcg cgtggaatcg atggtgtggc gcataaagct gccttacagg taaatggcgt
    20701 cagcattgct gtattgggga atggacttaa taccattcat ccccgccgcc atgcccgact
    20761 ggctgccagt ctgcttgaac atggcggagc tctcgtctcg gaatttcccc tcgatgttcc
    20821 accccttgct tacaatttcc cacgaagaaa tcgcattatc agtggtctaa gtaaaggtgt
    20881 actggtggtg gaagcggctt tgcgcagtgg ttcgctggtg acagcacgtt gtgcgcttga
    20941 gcagaggcgt gaagtttttg ccttgccagg tccaataggg aatccgggaa gcgaagggcc
    21001 tcactggtta ataaaacaag gtgcgattct tgtgacggaa ccggaagaaa ttctggaaaa
    21061 cttgcaattt ggattgcact ggttgccaga cgcccctgaa aattcatttt attcaccaga
    21121 tcagcaagac gtggcattgc catttcctga gctcctggct aacgtaggag atgaggtaac
    21181 acctgttgac gtcgtcgctg aacgtgccgg ccaacctgtg ccagaggtag ttactcaact
    21241 actcgaactg gagttagcag gatggatcgc agctgtaccc ggcggctatg tccgattgag
    21301 gagggcatgc catgttcgac gtactaatgt atttgtttga aacctatatt cacactgaag
    21361 ctgagttgcg tgtggatcaa gacaaacttg aacaggatct taccgacgca ggttttgagc
    21421 gagaagatat ctacaatgcc ctgctatggc tggaaaaact tgctgattat caggaagggt
    21481 tggcagaacc gatgcaactg gcctcggatc ctctctccat gcgtatttat acaccggaag
    21541 agtgtgaacg actggatgcc agctgccgtg ggtttctgct tttccttgag cagattcagg
    21601 tgctcaacct tgaaactcgt gaaatggtga tagagcgagt gctcgcgctg gataacgcag
    21661 agttcgaact ggatgatctg aaatgggtga tcctgatggt gttgttcaac atcccggggt
    21721 gcgaaaatgc gtaccagcaa atggaagaat tactctttga agtgaatgaa ggtatgctgc
    21781 attaatcatt tctttaattc agcataagtt gttatggcaa aatcagcact gttcacggtg
    21841 cgtaataatg agtcctgccc aaagtgcggg gctgaactgg ttattcgatc cgggaaacac
    21901 ggtccgtttc ttggatgctc acagtatccg gcgtgtgact acgtccgtct tctgaaatct
    21961 tcagcggatg gacatattgt caaagttctg gaggggcagg tttgccctgc atgtggcgca
    22021 aatctggtat tacgccaggg acgctttggt atgtttattg gttgcagtaa ctaccctgaa
    22081 tgcgaacata ccgaacttat cgataaaccg gacgaaacag caattacatg cccccaatgt
    22141 cggacgggcc atctggtcca gcgccgctcc cgttatggca aaacatttca ctcctgtgat
    22201 cgctacccgg agtgtcaatt tgccattaac ttcaaaccaa tagctggaga atgccctgag
    22261 tgtcattatc cgctactcat cgaaaagaaa accgcgcagg gtgtaaaaca cttttgtgcc
    22321 agtaaacaat gtggaaagcc ggtttcggcg gaataataac gtgaataata acctgcaagg
    22381 agacgctatc gcagctgcga tagatgtcct caatgaagaa cgtgtcatcg cctatccaac
    22441 ggaagccgtt ttcggtgttg ggtgcgatcc tgatagcgaa acagcagtga tgagactgtt
    22501 ggagttaaaa cagcgtccgg ttgataaggg gctgatttta atcgcggcaa attacgagca
    22561 gcttaaaccc tatattgatg acaccatgct gactgatgcg cagcgtgaaa ccattttttc
    22621 ccgctggcca ggtcctgtca cctttgtctt tcccgcgcct gcgacaacac cgcgctggtt
    22681 gacgggccgc tttgattcgc ttgctgtacg agtcaccgac catccgttgg tggttgcttt
    22741 gtgccaggct tatggtaaac cgctggtttc taccagtgcc aacttgagtg gattgccacc
    22801 ttgtcgaaca gtagacgaag ttcgcgcaca atttggcgcg gcgttcccgg ttgtgcctgg
    22861 tgaaacgggg gggcgtttaa atccttcaga aatccgcgat gccctgacgg gtgaactgtt
    22921 tcgacagggg taacataatg gaaacctatg ctgtttttgg taatccgata gcccacagca
    22981 aatcgccatt cattcatcag caatttgctc agcaactgaa tattgaacat ccctatgggc
    23041 gcgtgttggc acccatcaat gatttcatca acacactgaa cgctttcttt cgtgctggtg
    23101 gtaaaggtgc gaatgtgacg gtgcctttta aagaagaggc ttttgccaga gcggatgagc
    23161 ttactgaacg ggcagcgttg gctggtgcag ttaataccct caagcggtta gaagatggac
    23221 gcctgctggg tgacaatacc gatggtgtag gcttgttaag cgatctggaa cgtctgtctt
    23281 ttatccgccc tggtttacgt attctgctta tcggcgctgg tggagcatct cgcggcgtac
    23341 tactgccact tctttcccta gactgtgcgg tgacaatcac taatcggacg gtatcccgcg
    23401 cggaagagtt ggctaaattg tttgcgcaca ctggcagtat tcaggcgttg ggtatggacg
    23461 aactggaagg tcatgagttt gatctcatta ttaatgcaac atccagtggc atcagtggtg
    23521 atattccggc gatcccgtca tcgctcattc atccaggcat ttattgctat gacatgttct
    23581 atcagaaagg aaaaactcct tttctggcat ggtgtgagca gcgaggctca aagcgtaatg
    23641 ctgatggttt aggaatgctg gtggcacagg cggctcatgc cttccttctt tggcatggcg
    23701 ttctgcctga tgtagaaccg gtcatcaaac tattgcaaca ggaattgtca gcgtgaatca
    23761 ggccatccag tttccggata gggaagagtg ggacgagaat aaaagatgtg tatgttttcc
    23821 tgctctcgtg aatggtatgc aactgacatg tgcgatctct ggcgagagtc tggcgtatcg
    23881 ctttactgga ggtacgccag aacagtggtt agcgagtttt cgtcagcatc gctgggacct
    23941 ggaagaagaa gcggaaaact taattcagga acaaagtgaa gatgatcaag gttgggtctg
    24001 gttaccttga tccagatatt cgtccttcca tttcacgtag ttatttgcgg aatagcgtaa
    24061 cccagccttc tcttcatcac ttaacgggcg gatctgtttg acgggactac cgagatacag
    24121 atatccgctc tccagccgtt tattttgtgg gaccaggcta cccgcaccaa tcatcacatc
    24181 atcttctact attgcgccat caagtaaaat tgagcccatc ccaaccaaaa ctcgattgcc
    24241 aatggtgcag ccgtggagca tcaccttgtg accaacagtg acatcttcgc caatggttaa
    24301 tgggttgcca tctgggttgt acgaggattt atgagtgaca tgcaacatac tgccatcctg
    24361 gatattggtg cgtgctccga tctgtacata atgtacatct ccacgaatca caacgagcgg
    24421 ccagatcccc acatcatcag ccagacgaac gtcaccaatc acgacgctgc tatcgtcgat
    24481 cattacgcgc tggccgattt gtggaaaaag atcgcggtat gggcgtaaaa catcagacat
    24541 actcacctca acaataaatg atttcctaat gactttgggt gcattattag ccttgtgcaa
    24601 gtcttttagt atgcaaaaaa gcaccgtttt gtgtgcgaat gcagcaaaaa gggtgaaaaa
    24661 acaacaaaca gaaaaaaaga tcaaaaaagt acttgtgcaa aaaattggga tccctataat
    24721 gcgcctccgt tgagacgaca acgtgaaaca cttcacagga tggtcggaac aacgaagaga
    24781 aaaaattctg aaattcaggg ttgactctga aagaggaaag cgtaatatac gccacctcgc
    24841 gacagtgagc tgaaagccgc gtcgcaactg ctctttaaca atttatcaga caatctgtgt
    24901 gggcactcga agatacggat tcttaacgtc gcaagacgaa aaatgaatac caagtctcaa
    24961 gagtgaacac gtaattcatt acgaagttta attctttgag catcaaactt ttaaattgaa
    25021 gagtttgatc atggctcaga ttgaacgctg gcggcaggcc taacacatgc aagtcgaacg
    25081 gtaacaggaa gcagcttgct gcttcgctga cgagtggcgg acgggtgagt aatgtctggg
    25141 aaactgcctg atggaggggg ataactactg gaaacggtag ctaataccgc ataatgtcgc
    25201 aagaccaaag agggggacct tcgggcctct tgccatcgga tgtgcccaga tgggattagc
    25261 ttgttggtgg ggtaacggct cacc
//
LOCUS       DAZPDU010000045        25106 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZPDU010000045 DAZPDU010000000
VERSION     DAZPDU010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25106
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.045"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            196..384
                     /gene="bcsR"
                     /locus_tag="ACHURS_001712"
     CDS             196..384
                     /gene="bcsR"
                     /locus_tag="ACHURS_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFR1237472.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            396..1148
                     /gene="bcsQ"
                     /locus_tag="ACHURS_001713"
     CDS             396..1148
                     /gene="bcsQ"
                     /locus_tag="ACHURS_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFR1237473.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            1145..1309
                     /locus_tag="ACHURS_001714"
     CDS             1145..1309
                     /locus_tag="ACHURS_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HFR1237474.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPR"
     gene            1316..3763
                     /gene="bcsA"
                     /locus_tag="ACHURS_001715"
                     /pseudo
     CDS             1316..3763
                     /gene="bcsA"
                     /locus_tag="ACHURS_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            3774..6113
                     /gene="bcsB"
                     /locus_tag="ACHURS_001716"
     CDS             3774..6113
                     /gene="bcsB"
                     /locus_tag="ACHURS_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFR1237475.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKSDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVLRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            6120..7226
                     /gene="bcsZ"
                     /locus_tag="ACHURS_001717"
     CDS             6120..7226
                     /gene="bcsZ"
                     /locus_tag="ACHURS_001717"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFR1237476.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVIA
                     PSDARKITTSEGQSYGMFFALAANDRVAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPIGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            7208..10700
                     /gene="bcsC"
                     /locus_tag="ACHURS_001718"
                     /pseudo
     CDS             7208..10700
                     /gene="bcsC"
                     /locus_tag="ACHURS_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
     gene            10782..12770
                     /gene="hmsP"
                     /locus_tag="ACHURS_001719"
     CDS             10782..12770
                     /gene="hmsP"
                     /locus_tag="ACHURS_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFR1237477.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPIMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFAHPLPIEIFEESYLEEK"
     gene            12953..14239
                     /gene="dctA"
                     /locus_tag="ACHURS_001720"
     CDS             12953..14239
                     /gene="dctA"
                     /locus_tag="ACHURS_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFR1237478.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            14459..15955
                     /locus_tag="ACHURS_001721"
     CDS             14459..15955
                     /locus_tag="ACHURS_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFR1237479.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNIGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(16051..16980)
                     /gene="kdgK"
                     /locus_tag="ACHURS_001722"
     CDS             complement(16051..16980)
                     /gene="kdgK"
                     /locus_tag="ACHURS_001722"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFR1237480.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVCLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            17212..17979
                     /gene="pdeH"
                     /locus_tag="ACHURS_001723"
     CDS             17212..17979
                     /gene="pdeH"
                     /locus_tag="ACHURS_001723"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFR1237481.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHDLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            18049..20109
                     /locus_tag="ACHURS_001724"
     CDS             18049..20109
                     /locus_tag="ACHURS_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFR1237482.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPTAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(20291..21613)
                     /locus_tag="ACHURS_001725"
     CDS             complement(20291..21613)
                     /locus_tag="ACHURS_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1237483.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(22024..23037)
                     /gene="yhjD"
                     /locus_tag="ACHURS_001726"
     CDS             complement(22024..23037)
                     /gene="yhjD"
                     /locus_tag="ACHURS_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFR1237484.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(23086..23985)
                     /locus_tag="ACHURS_001727"
     CDS             complement(23086..23985)
                     /locus_tag="ACHURS_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1237485.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAMVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGQLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            24505..>25106
                     /locus_tag="ACHURS_001728"
     CDS             24505..>25106
                     /locus_tag="ACHURS_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFR1237486.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         5894 a         6366 c         6992 g         5854 t
ORIGIN      
        1 atttagcttg tgaatcaatt tagaaggtca caagacgtgt gattttagtc ttttcgtttt
       61 taactttttc tgttgcgtaa cggaaagtca aaaagtgagc aaattcccgt ctcgccgtaa
      121 acaataaacc ggcgaaatgc tcacggttag aattgtttca tgaacggtac ggttatttca
      181 tagggatcaa gcaaaatgaa taacaatgaa ccagatactc tgcctgatcc cgcgataggc
      241 tatatcttcc agaatgatat tgtggcgtta aagcaggcat tttcactgcc tgatattgat
      301 tatgccgata tttcccaacg cgaacagttg gccgcggcat taaaacgctg gccgttgctg
      361 gcagagtttg cgcaacaaaa gtaggggatt ggtgaatggc cgtactggga ttgcaggggg
      421 tgcggggagg cgtggggaca acaaccatca ccgccgcatt agcctggtca ttacaaatgt
      481 tgggagaaaa tgtcctggtg gtcgatgcct gcccggacaa cttgttgcgc ctgtcattta
      541 atgttgattt tacccaccgt cagggctggg ccagagcgat gctggatggc caggactggc
      601 gtgacgctgg gttgcgctac acctcgcagc tcgatttgct gccttttggt cagttatcca
      661 ttgaagaaca agaaaatcca cagcactggc aaacccggct gagcgatatt tgctccggct
      721 tacagcaact aaaagccagc gggcgttacc agtggatttt aatcgactta ccgcgtgatg
      781 cctcgcagat aacccaccag ctgctgagtt tgtgcgatca ctcgctggca atcgtcaatg
      841 tggatgccaa ctgccatatc cgactgcatc agcaagcgct gccggatggc gcacatattt
      901 tgattaatga cttccgtatt ggcagtcagg ttcaggacga tatttaccag ctttggttgc
      961 aaagccagcg ccgattactg ccgatgctca ttcatcgtga tgaagcgatg gctgaatgcc
     1021 tggcggctaa gcaaccagta ggtgaatatc gcagtgatgc gctggcggct gaagagatac
     1081 tgacgctggc gaactggtgc ctgttgaact actccgggct gaaaacgcca gtcgggagtg
     1141 catcatgagt atcctgaccc ggtggttgct tatcccgccg gtcaacgcgc ggcttatcgg
     1201 gcgttatcgc gattatcgtc gtcacggtgc gtcggctttc agcgcgacgc tcggctgttt
     1261 ctggatgatc ctggcctgga tttttattcc gctggagcac ccgcgctagc agcgtattcg
     1321 cgcagaacat aaaaacctgt atccgcatat caacgcctcg cgtccgcgtc cgctggaccc
     1381 ggtccgttat ctcattcaaa catgctggtt actgatcggt acatcgcgca aagaaacgcc
     1441 gaaaccgcgc aggcgggcat tttcaggtct gcagaatatt cgtggacgtt accatcaata
     1501 gatgaacgag ctgcctgagc gcgttagcca taaaacacag catcttgatg agaaaaaaga
     1561 gctcggtcat ttgagtgccg gggcgcggcg gttgatcctc ggtatcatcg tcaccttctc
     1621 gctgattctg gcgttaatct gcgttactca accgtttaac ccgctggcgc agtttatctt
     1681 cctgatgctg ctgtggggtg tagcgctgat cgtacggcgg atgccggggc gcttctcggc
     1741 gctaatgttg attgtgctgt cgctgaccgt ttcttgccgt tatatctggt ggcgttacac
     1801 ctctacgctg aactgggacg atccggtcag cctggtgtgc gggcttattc tgctcttcgc
     1861 tgaaacgtac gcgtggattg tgctggtgct cggctacttc caggtagtat ggccgctgaa
     1921 tcgtcagccg gtgccattgc cgaaggatat gtcgctgtgg ccgtcagtgg atatctttgt
     1981 cccgacttac aacgaaaatc tcaatgtggt gaaaaatacc atttacgcct cgctgggtat
     2041 cgactggccg aaagacaaac tgaacatctg gatcctcgat gacggcggca gggaagagtt
     2101 tcgccagttt gcgcaaaacg tgggggtgaa atatatcgcc cgcaccactc atgaacatgc
     2161 gaaagcgggc aacatcaaca atgcgctgaa atatgccaaa ggcgagttcg tgtcgatttt
     2221 cgactgcgac cacgtaccaa cgcgatcgtt cctgcaaatg accatgggct ggttcctgaa
     2281 agagaaacag ctggcgatga tgcagactcc acaccacttc ttctcgccgg acccgtttga
     2341 acgcaacctg ggacgttttc gtaaaacgcc gaacgaaggc acgctgttct atggtctggt
     2401 gcaggatggc aacgatatgt gggacgccac tttcttctgc ggttcctatg cggtgattcg
     2461 tcgtaagccg ctggatgaaa ttggcggcat tgctgtcgaa accgtgactg aagatgcgca
     2521 tacttctctg cgtttgcacc gtcgtggcta tacctccgcg tatatgcgta ttccgcaggc
     2581 ggcggggctg gcgaccgaaa gtctgtcggc gcatatcggt cagcgtattc gctgggcgcg
     2641 cgggatggta caaatcttcc gtctcgataa cccgctcacc ggtaaaggac tgaagtttgc
     2701 tcagcggctg tgctacgtca acgccatgtt ccacttcttg tcgggcattc cacggctgat
     2761 cttcctgact gcgccgctgg cgttcctgct gcttcatgcc tacatcatct atgcgccagc
     2821 gttgatgatc gccctattcg tgctgccgca tatgatccat gccagcctga ccaactcgaa
     2881 gatccagggc aaatatcgcc actctttttg gagtgaaatc tacgaaacgg tgctggcgtg
     2941 gtatatcgca ccaccgacgc tggtggcgct gattaacccg cacaaaggca aatttaacgt
     3001 caccgccaaa ggtggactgg tggaagaaga gtacgtcgac tgggtgatct cgcggcccta
     3061 catcttcctt gttctactca acctggtggg cgttgcggta ggcatctggc gctacttcta
     3121 tggcccgcca accgagatgc tcaccgtggt cgtcagtatg gtgtgggtgt tctacaacct
     3181 gattgttctt ggcggcgcag ttgcggtatc ggtagaaagc aaacaggtac gccgatcgca
     3241 ccgcgtggag atgacgatgc ccgcggcaat tgcccgcgaa gatggtcacc tcttctcgtg
     3301 taccgttcag gatttctccg acggtggttt ggggatcaag atcaacggtc aggcgcagat
     3361 tctggaaggg cagaaagtga atctgttgct taaacgcggt cagcaggaat acgtcttccc
     3421 gacccaggtg gcgcgcgtga tgggtaatga agttgggctg aaattaatgc cgctcaccac
     3481 ccagcaacat atcgattttg tgcagtgtac gtttgcccgt gcggatacat gggcgctctg
     3541 gcaggacagc tacccggaag ataagccgct ggaaagtctg ctggatattc tgaagctcgg
     3601 cttccgtggc taccgccatc tggcggagtt tgcgccttct tcggtgaagg gcatattccg
     3661 tgtgctgact tctctggttt cctgggttgt atcgtttatt ccgcgccacc cggagcggag
     3721 cgaaacggca caaccatcgg atcaggcttt ggctcaacaa tgatgataac gcgatgaaaa
     3781 gaaaactatt ctggatttgt gcagtggcta tggggatgag tgcgttcccc tctttcatga
     3841 cgcaggcgac gccagcaacg caaccactga tcaatgctga gccagctgta gccgcccaga
     3901 cggaacaaaa tccgcaggtg gggcaagtga tgccgggcgt gcagggcgct gatgcgccag
     3961 tcgtggcgca gaacggtcct tcgcgtgatg tgaagctgac ctttgcgcaa attgcgccgc
     4021 cgccgggcag catggtgcta cgtggcatta acccgaacgg cagcattgag tttggtatgc
     4081 gcagcgatga agtggtgacg aaggcgatgc tcaacctcga atacacgcca tcgccatcgt
     4141 tactgcctgt ccagtcgcag ttaaaggttt atctcaatga tgagctgatg ggcgtgctgc
     4201 cagtgaccaa agaacagttg ggtaaaaaaa cgctggcgca aatgcccatt aacccactgt
     4261 ttattaccga cttcaaccgt gtgcggctgg agtttgtcgg ccattatcag gacgtgtgcg
     4321 aaaacccggc cagcaccacg ctttggctgg atgttgggcg gagcagtgga ctggatctga
     4381 cctatcagac cctgaatgtg aagagtgacc tgtcacactt cccggtgcca ttctttgacc
     4441 cgcgcgataa ccgtactaac accttgccga tggtctttgc gggtgcgccg gatgttgggc
     4501 tgcaacaagc atctgccatt gtcgcctcgt ggtttggttc gcgttctggc tggcgtgggc
     4561 agaacttccc ggtgctctat aaccaattgc cggatcgcaa tgccattgtc tttgcaacca
     4621 acgacaaacg gccggacttc ctgcgcgatc atccggcggt aaaagccccg gtgattgaga
     4681 tgattaacca tccgcagaat ccttacgtca aactgctggt ggtgtttggt cgtgacgaca
     4741 aagacctgtt gcaggcagcg aaaggtatcg ctcagggtaa cattctgttc cgtggtgaaa
     4801 gcgtggtagt gaatgaagtg aaaccgctgc taccgcgtaa gccgtacgat gcgccgaact
     4861 gggtacgtac cgatcgtccg gtcacatttg gcgaactgaa aacctatgaa gaacagttac
     4921 aatccagcgg tcttgagcca gcagcgatta acgtttcgct aaacctgccg ccggatctct
     4981 acctgatgcg cagtaccggc attgatatgg atattaatta ccgctacacc atgccgccgg
     5041 tgaaagacag ttcgcggatg gatatcagcc tgaataacca gttcctgcaa tccttcaacc
     5101 tgagcagtaa acaggaggcg aaccgcctgc tgctgcggat tccggtatta caaggtttgc
     5161 tggatggcaa aacggatgtc tctattccgg cgctgaaact gggcgcgacc aaccagctgc
     5221 gcttcgactt tgagtatatg aacccgatgc cgggcggttc ggtggataac tgtattacct
     5281 tccagccggt gcagaatcat gtggtgattg gtgacgattc caccatcgac ttctcgaagt
     5341 attaccactt catcccgatg ccggatctac gcgcctttgc taacgcgggc ttcccgttca
     5401 gccggatggc ggatctgtcg caaaccatca ccgtgatgcc gaaaacgcct aacgaagcac
     5461 agatggaaac gttgctgaat actgttggtt ttatcggcgc acagacgggc ttcccggcga
     5521 ttaatctgac ggtgaccgat gatggcagca ccattcaggg caaagatgcc gacatcatga
     5581 tcatcggtgg tatcccggac aaattaaaag acgataagca gatcgacctg ttggtgcagg
     5641 cgaccgaaag ctgggtgaaa acaccgatgc gccagacccc gttccccggc attgtgccgg
     5701 acgagagcga tcgcgcggca gaaacccagt caacgctgac ctcttccggt gcgatggcgg
     5761 cggtgattgg cttccagtcg ccgtataacg accagcgcag cgtgattgcg ctgctggcag
     5821 atagcccacg cggttatgaa atgcttaacg atgcggtgaa cgatagcggc aaacgcgcca
     5881 ccatgttcgg ttcggtcgcg gtgctccgcg agtccggtat caacagccta cgtgttggcg
     5941 acgtttatta cgtaggtcat ctgccgtggt tcgagcgctt gtggtatgcg ctggcaaacc
     6001 atccgattct gctggcggtg ctggcggcaa tcagtgtgat attgctggca tgggtactgt
     6061 ggcgtctgct gcgaattatt agtcgtcgtc gtcttaaccc ggataacgag taattgaaga
     6121 tgaatgtgtt gcgtagtgga atcgtgacga tgctgctgtt ggctgccttt agtgttcagg
     6181 cagcctgtac ctggcctgcc tgggagcagt ttaaaaagga ttacatcagt caggaagggc
     6241 gcgtcatcgc ccccagcgac gcgcgcaaaa tcaccacctc cgaagggcaa agttacggta
     6301 tgttctttgc cctggcggct aacgaccgtg tagctttcga taatattctc gactggacgc
     6361 agaacaatct cgctcagggc tctttaaaag aacgtttgcc cgcctggctg tggggcaaga
     6421 aagagaacag taagtgggaa gtgctggaca gcaattcggc ctccgatggt gatgtctgga
     6481 tggcctggtc gttgctggag gcggggcgtt tgtggaaaga gcagcgttat accgacatcg
     6541 gcagcgcgtt gctaaaacgt atcgcgcggg aggaagtggt gacggtgcct gggctgggtt
     6601 ccatgttgtt accgggcaaa gtgggttttg ctgaggataa cagctggcgt tttaacccca
     6661 gctacctgcc gccgacgctg gcgcagtatt tcacccgctt tggcgcgccg tggaccacgc
     6721 tgcgcgaaac caatcaacgt ttattgctgg aaaccgcccc gaaaggtttt tcgccagact
     6781 gggtgcgcta tgagaaagac aaaggctggc agctaaaagc cgaaaaaaca ttgatcagca
     6841 gctacgacgc tatccgcgtt tacatgtggg taggcatgat gcctgacagc gatccgcaaa
     6901 aagcgcggat gctcaaccgg tttaaaccga tggcgacatt cactgagaaa aacggttatc
     6961 cgccggaaaa agtggatgtg gctacgggga aagcgcaggg taaaggaccg atcggttttt
     7021 ctgccgccat gctgcccttt ttacaaaacc gcgatgcgca ggccgttcag cgccagcgcg
     7081 tggccgataa ctttcccggc agcgatgcct attacaacta tgtgctgacc ctgtttggac
     7141 aaggctggga tcaacaccgt ttccgcttct cgacaaaagg tgagttatta cctgactggg
     7201 gccaggaatg cgcaaattca cactaaacat attcacgctt tccctcggtc tggccgtcat
     7261 gccgatggtc gaggcagcac caaccgctca gcaacagttg ctggagcaag ttcggttagg
     7321 cgaagcgacc catcgtgaag atctggtgca acagtcgtta tatcggctgg aacttattga
     7381 tccgaataac ccggacgtcg ttgccgcccg tttccgttct ttgttacgtc agggcgatat
     7441 tgatggcgcg caaaaacagc tcgatcggct gtcgcagtta gcgccgagtt caaatgcgta
     7501 taaatcgtcg cggactacga tgctactttc cacgccggat ggtcgtcagg cactgcaaca
     7561 ggcacgattg caggcgacga ccggtcatgc agaagaagct gtggcgagtt acaacaaact
     7621 attcaacggt gcgccgccgg aaggtgacat tgctgtcgag tactggagta cggtggcgaa
     7681 aattccggct cgccgtggcg aagcgattaa tcagttaaaa cgcatcaatg cggatgcacc
     7741 gggcaatacg ggcctgcaaa acaatctggc gctattgctg tttagtagcg atcgccgtga
     7801 cgaaggtttt gccgtcctgg aacagatggc aaaatcgaac gccgggcgcg aaggggcctc
     7861 taaaatctgg tacgggcaga ttaaagacat gcccgtcagt gatgccagtg tgtcggcgct
     7921 gaaaaaatat ctctcgatct ttagtgatgg cgatagcgtg gcggctgcgc aatcgcaact
     7981 ggcagaacag caaaaacagc tggccgatcc tgctttccgc gctcgtgcgc aaggtttagc
     8041 ggcggtggac tctggtatgg cgggtaaagc cattcccgaa ctacaacagg cggtgcgggc
     8101 gaacccgaaa gacagtgaag ctctgggggc gctgggccag gcgtattctc agaaaggcga
     8161 tcgcgccaat gcagtggcga atctggaaaa agccctcgca ctgaacccgc acagcagcaa
     8221 caacgacaaa tggaacagtc tgctgaaagt aaaccgctac tggctggcga tccagcaggg
     8281 cgatgctgcg ctgaaagcca ataatcctga ccgggcagaa cgcctgttcc agcaggcgcg
     8341 taatgtcgat aacaccgaca gttatgcagt gctggggctg ggcgatgtgg cgatggcgcg
     8401 aaaagattat cccgccgccg aacgttatta tcagcagacc ttgcgtatgg acagcggcaa
     8461 cactaacgcc gtgcgcgggc tggcaaatat ttaccgccag caatcgccag aaaaagctga
     8521 agcgtttatc gcctcgctct ctgccagtca gcggcgtagc attgatgata tcgaacgcag
     8581 cctgcaaaac gaccgtctgg cacagcaggc agaggcactg gaaaaccagg gcaaatgggc
     8641 gcaggcggca gcacttcagc ggcaacgact ggcgctggac cccggcagcg tatggattac
     8701 ttaccgactt tcgcaggatc tctggcaggc cggacaacgc agccaggccg atacgttaat
     8761 gcgcaatctg gcgcagcaga agtcgaacga cccagagcag gtttacgctt acgggctgta
     8821 cctacgctta cgggctgtac ctctctggtc atgaccagga cagagcggcg ctggcgcata
     8881 tcaacagcct gccgcgtgcg cagtggaaca gcaatattca ggagctggtt aatcgactgc
     8941 aaagcgatca ggtgctggaa accgctaacc gcctgcgaga aagcggcaaa gaggcagaag
     9001 cggaagcgat gctgcgccag caaccacctt ccacgcgtat tgacctcacg ctggctgact
     9061 gggcgcaaca acgacgtgat tacaccgccg cccgcgctgc atatcagaat gtcctgacgc
     9121 gggagccagc taacgccgac gccattcttg gtctgacgga agtggatatt gctgccggtg
     9181 acaaagcggc ggcacgtagc cagctggcga aactgcccgc taccgataac gcctcgctga
     9241 acacacagcg gcgcgtggcg ctggcacagg cgcagcttgg cgataccgca gcagcgcagc
     9301 ggacgtttaa taagttgatc ccgcaggcaa aatctcagcc accgtcgatg gaaagcgcga
     9361 tggtgctgcg tgatggtgcg aagtttgaag cgcaggcggg cgatccaacg caggcgctgg
     9421 aaacctacaa agacgccatg gtcgcatccg gtgtgactac gacgcgtccg caggataacg
     9481 acacctttac ccgactgacc cgtaacgacg agaaagatga ctggctgaaa cgtggcgtgc
     9541 gcagcgatgc ggcggacctc tatcgccagc aggatcttaa cgtcaccctt gagcacgatt
     9601 actggggttc gagcggcacc ggtggttact ccgatctgaa agcgcacact accatgttgc
     9661 aggtggatgc gccgtattct gacgggcgga tgttctttcg cagtgatttc gtcaatatga
     9721 acgtcggcag tttctccact aatgccgatg gcaaatggga tgacaactgg ggcacctgta
     9781 cattacagga ctgtagcggc aaccgcagcc agtcggattc cggtgccagc gtggcggtcg
     9841 gctggcgaaa tgacgtctgg agctgggata tcggtaccac gccgatgggc ttcaacgtgg
     9901 tggatgtggt cggcggcatc agttacagcg atgatatcgg gccgctgggt tacaccgtta
     9961 acgcccaccg tcggcccatc tccagttctt tgctggcctt tggtgggcaa aaagactccc
    10021 cgagcaatac cgggaaaaaa tggggtggcg tacgtgccga cggtgtgggg ctaagtctga
    10081 gctacgataa aggtgaagca aacggcgtct gggcatcgct tagtggcgac cagttaaccg
    10141 gtaaaaatgt cgaagataac tggcgcgtgc gctggatgac gggctattac tataaggtca
    10201 ttaaccagaa caatcgccgc gtcacaatcg gcctgaacaa catgatctgg cattacgaca
    10261 aagatctgag tggctactca ctcggtcagg gcggttacta cagtccgcag gaatacctgt
    10321 cgtttgccat accggtgatg tggcgggagc gcacggaaaa ctggtcgtgg gagctggggg
    10381 cgtctggctc gtggtcgcat tcacgcacca aaaccatgcc gcgttatccg ctgatgaatc
    10441 tgatcccgac cgactggcag gaagaagctg cgcggcaatc caacgatggc ggcagcagtc
    10501 agggcttcgg ctacacggcg cgggcattac ttgaacgacg tgttacttcc aactggtttg
    10561 ttggcacggc aattgatatc cagcaggcga aagattacgc acccagccat ttcctgctct
    10621 acgtacgtta ttccgccgcc ggatggcagg gtgacatgga tttaccgccg cagccgctga
    10681 taccttacgc cgactggtaa gttttcagat agcgcctctc ttaatgccgc tgcgatcggg
    10741 tatactcggg cggcaatctg ggatttccgg ggggagacaa tttgcgcgta agtcgctcgt
    10801 taacaatcaa gcagatggca atggtggcag ccgttgtcct ggtgttcgtt tttatttttt
    10861 gcaccgtttt gctgttccat ctggtccagc agaatcgcta taacacggct acgcaactgg
    10921 aaagcattgc tcgctctgtc cgcgaaccct tatcttcagc tattttgaaa ggcgatattc
    10981 ccgaagcgga agctattctt gccagcatta aaccggcagg cgtggtcagc cgtgccgatg
    11041 tagtgctgcc taaccagttc caggcgctgc gtaaaagttt tattccagag cgcccggtgc
    11101 cgataatggt tactcgcctg tttgagctac cggttcaaat ctcgctgggc gtttactcgc
    11161 tcgaacgtcc ggcaaacccg cagccaattg cctatctggt actacaggcg gattccttcc
    11221 gtatgtataa gttcgtgatg agcaccctct caacgttagt gaccatttac ttacttttgt
    11281 cgcttatcct gaccgtcgcc atcagctggt gcattaaccg cctgattttg catccgttac
    11341 gcaatattgc tcgcgaactt aacgccatcc cagcccagga gcttgttggt caccaactgg
    11401 cattaccgcg tctgcatcag gacgatgaaa tcggtatgtt ggtgcgcagt tacaacctca
    11461 accagcaatt gctgcagcgc cattatgaag aacagaacga aaatgcgatg cgcttcccgg
    11521 tgtcggattt gccgaacaaa gccttgctga tggagatgct ggagcaggtt gtcgcgcgta
    11581 aacaaaccac cgcgctgatg atcatcacct gtgaaaccct gcgtgatact gcgggcgtgc
    11641 tgaaagaggc gcaacgagaa attctgctgc tgacgctggt ggaaaaactc aaatcggtac
    11701 tgtcgccacg tatgatcctc gcgcagatta gcggttatga ctttgctgtc attgccaacg
    11761 gtgtacagga accgtggcac gcaatcacct taggtcagca agtgctcact atcatgagcg
    11821 agcgcctgcc gattgaacgt attcaactcc gtccgcactg tagcattggc gtggcgatgt
    11881 tctacggcga tctcaccgcc gaacagcttt acagtcgcgc tatttctgcg gcatttaccg
    11941 ctcgccataa aggcaagaat cagattcagt tctttgatcc gcagcagatg gaagccgccc
    12001 agaagcggtt gacggaagag agcgatatcc ttaatgcact ggaaaatcat cagtttgcta
    12061 tttggttaca gccacaggtc gagatgacca gcggtaaact ggtcagtgcg gaagtgttac
    12121 tgcgtatcca gcaaccggat ggcagttggg acctgccgga tggcttaatc gatcgcattg
    12181 agtgctgtgg gctgatggtt accgtcggtc actgggtgct ggaagagtcc tgtcgattgc
    12241 ttgcagcctg gcaagagcgc ggcattatgc tgcccttgtc ggtaaacctc tctgcgctgc
    12301 aactgatgca cccaaatatg gtggcggata tgctggaact gttaacccgc tatcgcattc
    12361 agccgggaac actgattctg gaagtgacag aaagccgacg tattgacgac cctcatgctg
    12421 cggtggcaat cctccgtccg ctgcgcaatg ccggagttcg ggtggcgctg gatgatttcg
    12481 gcatgggcta cgcagggctg cgtcagctgc agcatatgaa atcgttgcca atcgacgtac
    12541 tgaaaatcga caaaatgttt gttgaaggct tgccggaaga tagcagcatg attgctgcaa
    12601 ttatcatgct ggcgcagagc ctgaacttac aaatgattgc cgaaggcgtg gagactgaag
    12661 cacaacgcga ctggctggca aaagcgggcg ttggtattgc ccagggcttc ctttttgctc
    12721 acccactccc tattgaaatc ttcgaagaga gttacctgga agaaaagtag ctaccccaaa
    12781 ctgattacaa aactttaaaa agtgctggtt tgtgcgagcc agctcaaact ttttaacctt
    12841 tttgtttcaa ttatgatcca ggtacatttc tgtgatgttg tctgggtgtt attttaaggc
    12901 cgcaggtacc ccataacctt acaagacctg tggttttact aaaggacacc ctatgaaaac
    12961 ctctctgttt aaaagccttt actttcaggt cctgacagcg atagccattg gtattctcct
    13021 tggccatttc tatcctgaaa taggcgagca aatgaaaccg cttggcgacg gcttcgttaa
    13081 gctcattaag atgatcatcg ctcctgtcat cttttgtacc gtcgtaacgg gcattgcggg
    13141 catggaaagc atgaaggcgg tcggtcgtac cggcgcagtc gcactgcttt actttgaaat
    13201 tgtcagtacc atcgcgctga ttattggtct tatcatcgtt aacgtcgtgc agcctggtgc
    13261 cggaatgaac gtcgatccgg caacgcttga tgcgaaagcg gtagcggttt acgccgatca
    13321 ggcgaaagac cagggcattg tcgccttcat tatggatgtc atcccggcga gcgtcattgg
    13381 cgcatttgcc agcggtaaca ttctgcaggt gctgctgttt gccgtactgt ttggttttgc
    13441 gctccaccgt ctgggcagca aaggccaact gatttttaac gtcatcgaaa gtttctcgca
    13501 ggtcatcttc ggcatcatca atatgatcat gcgtctggca cctattggtg cgttcggggc
    13561 aatggcgttt accatcggta aatacggcgt cggcacactg gtgcaactgg ggcagctgat
    13621 tatctgtttc tatattacct gtatcctgtt tgtggtgcta gtattgggtt caatcgctaa
    13681 agcgactggt ttcagtatct tcaaatttat ccgctacatc cgtgaagaac tgctgattgt
    13741 actggggact tcatcttccg agtcggcgct gccgcgtatg ctcgacaaga tggagaaact
    13801 cggctgccgc aaatcggtgg tggggctggt catcccgaca ggctactcgt ttaaccttga
    13861 tggcacatcg atatacctga caatggcggc ggtgtttatc gcccaggcca ctaacagtca
    13921 gatggatatc gtccaccaaa tcacgctgtt aatcgtgttg ctgctttctt ctaaaggggc
    13981 ggcaggggta acgggtagtg gctttatcgt gctggcggcg acgctctctg cggtgggcca
    14041 tttgccggta gcgggtctgg cgctgatcct cggtatcgac cgctttatgt cagaagctcg
    14101 tgcgctgact aacctggtcg gtaacggcgt agcgaccatt gtcgttgcta agtgggtgaa
    14161 agaactggac cacaaaaaac tggacgatgt gctgaataat cgtgcgccgg atggcaaaac
    14221 gcacgaatta tcctcttaat ctcaccactt atgcccgcac tcccttcccc gagtgcgggt
    14281 gtttgcgcat aattgacccc tgtgcctgaa cttaccacct gggtgagttc aggtttattt
    14341 cacttcttcc gtgacatttt catgttcttg cggtctaaca cgaagtgttt ttaacgtcat
    14401 ttagactgac tgacaacggg cagtttttta ttaccaggat agttgatcag gggttcacat
    14461 gcagggcaca aaaattcgac ttttagcggg cggtttgctg atgatggcca ctgctggcta
    14521 tgtgcaggca gatgcgctcc agcctgatcc agcatggcaa caggggacgc tttccaacgg
    14581 tttacagtgg caagtgctga ccacccccca gcgtcccagc gatcgtgttg aaattcgcct
    14641 gctggttaat atcggttcgc tcgccgaaag tacacaacag agcggttaca gtcacgccat
    14701 ccctcgtatt gcgctaacgc aaagcggtgg ccttgacgca gcacaggcgc gttcattgtg
    14761 gcagcagggg atcgacccta aacgcccgat gccgccggta attgtctctt atgacaccac
    14821 gctgtttaat ctgagtttgc ccaataaccg taacgatttg ctgaaagaag cgctctctta
    14881 tctggcaaat gccactggca aattgaccat cacaccagaa accatcaacc acgcgctgca
    14941 aagtcaggac atggtggcaa cctggcctgc cgatactaaa gagggctggt ggcgctatcg
    15001 tctgaaaggg tcaaccttgt taggtcacga tcctgccgat ccgctgaaac aacccgttga
    15061 agcggaaaaa attaaagatt tctatcagaa atggtacacc ccggatgcaa tgacgctact
    15121 ggtggtggga aacgtggatg cgcgctcggt tgtcgaccaa atcaacaaaa cgtttggcga
    15181 actgaaaggc aaacgtgaaa cgccagctcc ggtgccgacg ctttctccgc tgcgtgcgga
    15241 agcggtgagt attatgactg acgcggtgcg tcaggaccgg ttatctatca tgtgggatac
    15301 gccgtggcag ccgattcgtg aatcagccgc actgctgcgc tactggcgtg cggacctggc
    15361 ccgcgaggcg ctgttctggc atgttcagca agcgttaagc gccagtaaca gcaaagacat
    15421 cggtcttgga tttgactgcc gtgtgctgta tctgcgtgcg cagtgtgcca tcaacatcga
    15481 atcaccaaac gacaagctga acagcaacct taatctggtg gcgcgtgaac tggcgaaggt
    15541 tcgcgataaa ggtctgccgg aagaagagtt caatgcgtta gtggcgcaaa agaaactgga
    15601 gctgcagaaa ctgtttgccg cctatgcgcg ggctgatacc gatattctga tgggtcagcg
    15661 gatgcgttcg ctgcaaaatc aggtcgtcga tatcgcgccg gagcagtatc agaaactgcg
    15721 gcaggatttc cttaacagcc tgacggtgga gatgttaaat caggatctgc gtcagcagtt
    15781 gtcgaatgat atggcgttaa tactgctgca gccgaaaggc gagccggaat ttaacatgaa
    15841 agcgttgcag gcggcctggg atcaaatcat ggccccatcg actgctgctg ccgccacctc
    15901 tgtcgccacg gatgacgtac atcctgaagt gacggatatt ccacctgcac agtaatgcct
    15961 gatggtgatg agttagcaca accatcaggt atttatggat gagctggata gaggggtaac
    16021 actttatcca gccttttgca tatgctgcgt ttacgctggc atcgcctcac gcgggataat
    16081 cgcgccgcga tactgaataa cggtacttgc ggtcaggtgc ccgcgtttcg ccgcgttttc
    16141 cgcactgccg cccgtcaaac ataccgccag ataaccggcg ctgaaagagt cgccagctgc
    16201 ggtggtatcg atcacttttt ctttcggcag tttcaccgcc ggaacatcca ctaacccttc
    16261 gccagcaatg gacaccaggc aagaatccgc cccgcgtttc accaccactt ctttcacgcc
    16321 cgcgttatgg gtgcgcgcaa tgacgtcttc caccggctgt tgaccccaca gcgcgtcttc
    16381 atcgtccagc gtcaggaagg cgatatccgt gcattccagc atttgttggt acacctgctg
    16441 tgtctcttct ttgctggccc acaggcgcgg acgatagtta ttgtcgaaaa tcacttttcc
    16501 gccgttggcg cggcattcgc gcagcaggga aagcagcttt tcgcggctgg tcgggcttaa
    16561 gatcgccagg ctaatcccgc tcaggtagag ataatcgaaa ttcgccagct cttcgcaaat
    16621 cgccgcagac tgctcactct ccagccagaa tttggcggcg gcttcgttcc gccagtagta
    16681 gaacgtacgc tcgccggtgc tgtcggtttc aatgtagtaa aggcccggca gacggttttc
    16741 catccgttgg gtcagggaag tatcaacgtt ctcgccgtgc caggcgtcca gcatttgctg
    16801 gctaaaactg tccgttccca gcgccgttac gtaatgaacg gttaatgccg caggatcgac
    16861 ctgacgggcg atatagacgg aagtgttcag ggtatcgccg ccgaaaccgc gcttaacgtc
    16921 cgcgcctttc tcggaaagct caatcatgca ttcgccaatc acggcaatct ttttggacat
    16981 agtcgtgaac ctgatctgta aaaaattagc gttagtttgc gctgtggtgg tttgctggtc
    17041 aaccatatta aaacagtgtt ccattatttt tttgagccag aacgtattcc tgaaagattc
    17101 cgttgtggag tgaggaaatt taccccggcc aggcggtaaa gttctgccct tgcgcgccga
    17161 taatctttgt cgagtccggg cagcatcact tttaaacaca ggacatcttt gatgataagg
    17221 caggttatcc agcgaataag caaccctgaa gcaagcatcg agagcttgca ggaacggcgt
    17281 ttttggttgc agtgtgagcg tgcttacacc tggcagccga tctatcaaac atgcggacgg
    17341 ttaatggccg tggagctatt aacggtggtc acgcatccct tgaacccttc gcaacgcctg
    17401 ccgccggatc gctattttac tgaaatcacc gtcagccatc ggatggaggt tgtgaaagag
    17461 cagattgatt tgctggcgca aaaagccgac ttctttgtag agcacgacct gctggcatcg
    17521 gtcaatattg atggccctac gctcatcgcc ctgcgtcagc aaccaaaaat cctgcgccaa
    17581 attgagcgtc ttccctggct gcgtttcgaa ctggtggaac acatccgcct gccgaaagat
    17641 tcaacctttg cctcgatgtg tgaatttggc ccgctgtggc tggatgattt tggcaccggg
    17701 atggcaaatt tctctgcgct aagtgaagtg cgttatgact acatcaaaat cgcgcgagag
    17761 ctgttcgtga tgctgcgtca gtcgccggaa ggccgcacac tcttttctca gcttttacat
    17821 ctaatgaatc gctattgtcg cggggtgatt gtcgagggcg tagaaacacc ggaagagtgg
    17881 cgtgatgttc agaactcgcc cgcattcgcc gcacaaggct ggtttctttc acgcccggca
    17941 ccgatggaaa cgctgaatac ggcggttctg gcgctataag ctgcctcatt ttccgctggg
    18001 ctggactatc tttaggactg gcacaggaag aatatgagga agcgaacgat gagcaaggca
    18061 ggcaaaataa ccgctgcgat ttcaggggct ttcttgttgt tgattgtcgt ggcgatcatt
    18121 ttgattgcaa cttttgactg gaatcgactc aaaccgacca tcaaccagaa agtctctgcg
    18181 gagttgaatc gtccgttcgc tatccgtggc gatctgggcg tggtgtggga gcggcaaaaa
    18241 caagaaactg gctggcgcag ctgggtgccg tggcctcatg tacatgcaga agacatcatt
    18301 cttggtaatc caccggatat tcccgaagtc acgatggtgc atttgccgcg agtagaggca
    18361 acgctggcac cgctggcgct gctgaccaaa acggtctggc tgccgtggat caagctcgaa
    18421 aagcccgacg cgcgcctgat tcgcctctcc gaaaagaaca ataactggac gtttaatctt
    18481 gccaacgatg acaacaaaga cgcgaatgca aagccgtcgg catggtcgtt tcggctggat
    18541 aatattcttt tcgatcaagg gcggatcgcc attgatgaca aagtaagcaa agcggatctg
    18601 gaaatttttg ttgatccgtt aggcaagccg ctgccgttca gcgaagttac tggatcgaaa
    18661 ggtaaagcgg ataaagaaaa ggtgggcgat tacgtttttg gcctgaaggc gcagggacgt
    18721 tataacggtg aaccgctcac gggtacggga aaaataggcg gtatgctggc gctgcgtggc
    18781 gaagggacgc cgtttccggt acaggctgat ttccgttcag gtaatacccg tgttgctttt
    18841 gatggcgtcg tgaatgaccc aatgaagatg ggcggtgtcg atttacggct taaattttct
    18901 ggcgattcac tgggtgatct ctatgaactg acgggcgttc tgctgcccga taccccgccg
    18961 tttgaaacgg atggtcggct ggtagcgaaa atcgacactg aaaaatcgtc ggtctttgat
    19021 tatcgcggtt ttaatgggcg aattggcgat agcgatatcc acggttctct ggtctacacc
    19081 accggaaagc cacgaccaaa actggaaggt gatgtcgagt cgcggcaatt gcggctggcg
    19141 gacctgggac cgttgattgg cgttgattcc gggaaagggg cagaaaagtc gaaacggtct
    19201 gaacagaaga agggcgaaaa aagcgttcag cctgcgggca aagtgctgcc ttatgaccgc
    19261 ttcgaaaccg ataaatggga cgttatggat gccgatgttc gcttcaaagg gcggcgcatt
    19321 gagcatggca gtagcctgcc gattagcgat ctttctactc atatcatcct caaaaatgct
    19381 gacctgcgcc tgcaaccgct gaaatttggc atggcgggcg gcagcattgc ggcgaatatt
    19441 catctggaag gcgataaaaa gccgatgcag gggcgggcag atattcaggc tcgtcgactg
    19501 aaactgaaag aactgatgcc cgatgtggaa ctgatgcaga agacgctggg ggaaatgaac
    19561 ggtgacgcgg aactacgcgg tagcggtaac tcggtggcgg cgcttttagg caacagtaac
    19621 ggcaacctga aactgttgat gaatgacggg ctggtgagcc gcaacctgat ggagattgtt
    19681 gggctgaatg tcggcaacta cattgtcggt gcgatatttg gtgacgatga ggtgcgggtg
    19741 aactgcgcgg cggcgaatct gaatattgcc aacggcgtgg cgcgcccgca gatttttgct
    19801 ttcgatactg agaacgcgtt gattaatgtt accggcacgg caagttttgc ttcagaacag
    19861 ctggatttga ctattgatcc ggagagtaaa gggattcgga ttatcacact gcgttcgccg
    19921 ctgtatgtgc gtgggacgtt taaaaatccg caggctgggg tgaaagccgg accgctgatt
    19981 gcccgtggtg ctgttgctgc ggcactggcg acgctggtaa caccgacggc ggcgttactg
    20041 gcactgatct caccttccga aggggaggct aatcagtgtc ggacgatatt gtcgcagatg
    20101 aagaagtgat gataattgcc ggatgtctga catccggcaa ttaaacagat ttagattttt
    20161 ggcaaagtgt gctttgttca tgccggatgc ggcgtgaacg ccttatccgg cctacaaatc
    20221 cttgtgaatt caatatattg tatggaaatt cagtcctgat aagcgtagcg catcaggctt
    20281 ttcacactta ttacaacgac tgatgtcgcg tctcatgggt cagcagcagg gcgattaacg
    20341 tcaagccagc catcgccgcc agatataacc ccaccgcacc taacccgtag ttagtctgca
    20401 accaggctgc gatatacggt gcaacggaag ccccgagaat cgacgctacg ttgtaagaga
    20461 atgatgctcc ggtgtaacgc acttctgttg ggaacagctc tggtaacagc gcgcccatcg
    20521 gcccgaaggt cagccccatc agacttaacc ccagcagcag gaaggcaaaa accagaatcg
    20581 ggttgccaga accgagcagc gggttaaagg cgaacagcgc gaacaggatg atcagcgtgg
    20641 tgataattac catgctttta cggcgaccaa aggcatcagc cagtaatccg gcgactggca
    20701 ccatcacgcc aaaaccaata actgccatca tcagcatcca caacacttcg ttacgcggca
    20761 ggccaagccc aactggcgcg gcggcggtac tgaaggtcat agagtagacc gtcatgatgt
    20821 aaaacagcgt ataggttgcc agcatgatga acgtacccag tacggttacg cgaacatgtt
    20881 tggtcagcag cgtacccagc gggatcttca cctgtttttt cgctttagcg actttctcaa
    20941 acaccggcga ctcatgcagc gacacgcgaa catacaggcc gataatgacc agtaccgccg
    21001 agaagataaa tggcacacgc cagccccagc tcataaactg ctcgtcggtc agtagccagg
    21061 aaagcagcaa gaaagtgccg ttggcaaaga agaagccaat cggtgcgccc agctgtggga
    21121 aggagccata cagtgcacgt ttgcgcggtg gggcgttttc agtcgccagc agcgccgcgc
    21181 cgccccattc accgcctaag cccagaccct gaccaaatcg agccagcgcc agcagcagcg
    21241 gggcgaaaat accaatcgtg gcatagcccg gcagcagacc aatcaccacg gttgaaatcc
    21301 ccatcgttag caacgaggcg accagcgtcg ctttacgccc aacgcgatcg ccaaaatgac
    21361 caaaaacggc agagccaatg gggcgcgcga cgaaggcgat ggcgaaggtg gcgagcgact
    21421 gtagcgttgc tgccgttgga tcgccctgcg ggaagaagat atgcggaaac acaataacgg
    21481 ccgcagtggc gtaaatgtaa aagtcgaaga actcaatggc cgtgccaatg agagaggcga
    21541 caaggacttt attacgcgag ttgatcggcg tgtattcttg ctcgtggtcg agtgttgtgg
    21601 ctgttgcttg cataatcttt tcttattttt tggctaacga atagccatat tacgcacagc
    21661 aaaagtgata tttcaatgtg taacaaatcg cggaaatggt caaaaaccag gcaaaaaggg
    21721 gataatgttt aggccagtga tttttcttct atgaaatgct tcacataatt ttataaatag
    21781 tggataaaac tggtttcagg ttaaataaaa gttaccaact ggatttatat gctggggtta
    21841 tgagttggaa tgaaattgtt tgctcccaaa tagatggctg aagaggctct ggggaagagt
    21901 ttcaagaggt tatgtgcgca ttatagccgg atgcgatgct ggcgcatctt atccggccta
    21961 cggggagcca ggccgaataa aatcgtatgc cggataaggc gtttacgccg catccggcct
    22021 attttaaggc tgcgttttcc ctggcattcg cgggtcgtct ttatattcgg cggtggcaat
    22081 ccacgccgcg caaaacagcg tcaaacgggc gaagaagtag aaaaatgcca tcagccccag
    22141 cacggaacca aatgctgcgc cagaggggga tttcatcagc gatggcaggg tgtaggtcat
    22201 cacgatttta atcacttcaa aaccaatagc agccagaaat gttccgcgaa tcagggcttt
    22261 tttgcgcgga cggtggcgcg gtaaccgcca gaagatccag aagaaaagca gatagttggc
    22321 gaagatggaa atcgccaggc caatcaaccg ccacgtcggt ttcagccact caatgctgtt
    22381 caggtgcagg gcgctaataa tcatttgctg cgccgaaccg gcaaccgagg tgatcgaaag
    22441 cgtcacaatc agcgcaatca acaaaccaat cagcgaaata aaatcacgta gatattttac
    22501 ccagaacttc tcctgatctt gcggcgagcg ttcccagaca tcgcgcgact gggcgcgaat
    22561 cgcttcacgc aggttaccca tccagttgat gccggaataa agcgccacag ccaggccgac
    22621 aagccctaca gtcgtacgct gctgaacggc ggtgttgatg gtgtttttca acgtggcggc
    22681 tagcgtcgga tcgctgatgt tttgcagaat tttgtcgaag atatcctgta gcaacatcgg
    22741 atgggaggcc agcacaaagc cccctgcggc aaacgacacc atcaaaatcg ggatcatcga
    22801 caaaaatgag aaataggtga tagccgcacc aaactggtta cccagccgat cgttaaagcg
    22861 ttctgtcgcg cgaatcaggt gcgcaataac cggttggcgt tggacttttt cggcagtggt
    22921 ggtgacggtt tccagcgcct ggctaacttt gccgcctttc tcgctattat ccaactgctt
    22981 aatcggctcg tgctccagat cctgggtggg acgtttgatc tcgttttcct gcgtcatccg
    23041 ctcttttgtc cttctgttgt ggtcattctt aaaaagtata gtcggtcagt ccaggtactc
    23101 tttcattacg ccagccagcc actgcataaa caggtttaca cgccgggaaa gctcccgacg
    23161 ctgcggataa accagcgaaa gggagagcgg ctcggcacgg tagcctggca acacttcaat
    23221 aagctgcccg gcacgcaggg cttcgcgcac ggcaatgcgc gggatctgaa taatccccag
    23281 accggcaaga ccggcggcga gatagttttc gctactgttt accgtcaaca tgccgccaga
    23341 cttaaaccac tggacgccat taacgctggc aacctcaaaa cctaacggat gtacacccag
    23401 gtgcggtgta taacgcacca ttgcgtgtga agtcagatca tcggggcttt gcggataacc
    23461 aaagcgcgtc agatagtgcg gactggcaca gttgaccatg gtcagtttgc cgaggggacg
    23521 ggcgataacg ccatcttccg gtaacgcgcc agtgcgtatc acgcaatcaa aaccatcatg
    23581 aagaatatct accggacggt tatggctact cagttccagc tcaattcccg gatgctgata
    23641 gagaaattcc gacagacgcg gcagtaacag gtttttcgcg attcctgacg ggatgtcgat
    23701 gcgtaattta ccgctgatac tggtggcatc ctgttgaaac agaccgtcca gttcgctgag
    23761 attactcaac acatcttttg cccgttgata ataggtcatg ccttctggcg tgagtttgac
    23821 ccgtcgcgta gtgcgctgga gaagctgggt gccaagttga tgttccagtg cctgtatctg
    23881 gcgcgaaaca cttccctttg gcaaagcaaa gaaatccgcc gcgcgggaaa aactttccag
    23941 ctccgcgact ttgatgaaca actgcattgc gtgaatttta tccatttctt tgaccgattg
    24001 ttgttcacag cgaaacagta acgcgcatat cgcattgttt attgtttttt ctaacatcta
    24061 atgccgggtt tcacaatgtt gtcatgacga atgtggcgat ttctcagcag gaaaagggcg
    24121 gaatagcttt atgagaaagg tgaatcccct taaacagagc gtatttttca gtgattccct
    24181 ttaaagtgaa tagaggacgt attggctatt tgtgcttatt tttgtctcat tgccatttgt
    24241 tattttggct tgtagtcgcg gaatttcatt tttaattgca tgcgtactaa ccaccaatat
    24301 aaggaaatcc tcaaaattgt tttaaatagt gccctcgaca tgtaatttca ggcgtttagc
    24361 tgtaactatt tatatcggta actattttct acgcgtcatc tctgtagaat ggccaccgcc
    24421 aaatggcgaa ttcttatttt ttctcgcggg taataccctt ccactactta agtcttttta
    24481 acgtattcag gtggagattc agttatgcaa atagtcatgt ttgacaggca gtcaatattt
    24541 attcatggaa tgaaaatcag tttacagcag cgtattccag gagtgagtat tcagggggcc
    24601 agtcaggcag acgagttatg gcaaaagctg gaaagttacc ctgaagcctt agttatgctc
    24661 gatggcgatc aggatggtga gttttgctac tggttgctgc aaaaaaccgt ggtgcagttt
    24721 cctgaggtta aggtgttaat cacggcgacg gattgcaata aacggtggtt acaggaagtt
    24781 attcatttta atgtgctggc cattgtgcct cgtgattcaa ccgtcgagac atttgcgttg
    24841 gcggtaaata gtgcggcgat ggggatgatg tttctgcctg gagactggcg gacgacgccg
    24901 gaaaaggaca ttaaagatct caaatcgtta agcgcccgcc agcgggagat tttaaccatg
    24961 ttagcggcgg gcgaatcaaa taaggagatc ggcagagcgc tgaatatcag taccggaacg
    25021 gtaaaagcgc atctggaatc cctgtatcgc cgtctggaag tgaaaaatcg cacccaggcg
    25081 gcaatgatgt taaatatctc ctcctg
//
LOCUS       DAZPDU010000046        25093 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZPDU010000046 DAZPDU010000000
VERSION     DAZPDU010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25093
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.046"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..807
                     /locus_tag="ACHURS_001729"
     CDS             <1..807
                     /locus_tag="ACHURS_001729"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFR1237487.1"
                     /translation="DENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHI
                     DAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV
                     FNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIA
                     KLGPYEFICAGRPDEGIPAVCFKLKEGEDPGYTLYDLSERLRLRGWQVPAFTLGGEAT
                     DIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            963..2498
                     /gene="gadC"
                     /locus_tag="ACHURS_001730"
     CDS             963..2498
                     /gene="gadC"
                     /locus_tag="ACHURS_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFR1237488.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2629..3948
                     /locus_tag="ACHURS_001731"
     CDS             2629..3948
                     /locus_tag="ACHURS_001731"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFR1237489.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            4320..5702
                     /gene="dosC"
                     /locus_tag="ACHURS_001732"
     CDS             4320..5702
                     /gene="dosC"
                     /locus_tag="ACHURS_001732"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFR1237490.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVHSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            5727..8125
                     /gene="dosP"
                     /locus_tag="ACHURS_001733"
                     /gene_synonym="pdeO"
                     /pseudo
     CDS             5727..8125
                     /gene="dosP"
                     /locus_tag="ACHURS_001733"
                     /gene_synonym="pdeO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
     gene            8384..8965
                     /gene="ddpX"
                     /locus_tag="ACHURS_001734"
     CDS             8384..8965
                     /gene="ddpX"
                     /locus_tag="ACHURS_001734"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFR1237491.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFSCFISPGTQHVS"
     gene            8979..10529
                     /locus_tag="ACHURS_001735"
     CDS             8979..10529
                     /locus_tag="ACHURS_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFR1237492.1"
                     /translation="MKRSISFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDTPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            10531..11553
                     /locus_tag="ACHURS_001736"
     CDS             10531..11553
                     /locus_tag="ACHURS_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1237493.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYFSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            11550..12446
                     /gene="ddpC"
                     /locus_tag="ACHURS_001737"
     CDS             11550..12446
                     /gene="ddpC"
                     /locus_tag="ACHURS_001737"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFR1237494.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            12443..13429
                     /locus_tag="ACHURS_001738"
     CDS             12443..13429
                     /locus_tag="ACHURS_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1237495.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGYVHVLNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAITLLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISI"
     gene            13458..14354
                     /locus_tag="ACHURS_001739"
     CDS             13458..14354
                     /locus_tag="ACHURS_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016153094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFR1237496.1"
                     /translation="MNFPARKSWLGKTTEHVHAINGIDLQIRRGETLGIVGESGCGKS
                     TLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLSVWRIITEPLWIAKRSS
                     EQQRRALAKELAVQVGIRPEYLDRLPHAFSGGQRQRIAIAIARALSSQPDVIVLDEPT
                     SALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYLGQIVELGDAQ
                     QVLTAPAHPYTRLLLDSLPAIDKPLEEEWALCKTDLPGNRTLPQGCFFYERCPLATHG
                     CEVRQSLAIREDGRELRCWRAL"
     gene            complement(14410..14841)
                     /gene="osmC"
                     /locus_tag="ACHURS_001740"
     CDS             complement(14410..14841)
                     /gene="osmC"
                     /locus_tag="ACHURS_001740"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFR1237497.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTQSGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDADFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            15186..15401
                     /gene="bdm"
                     /locus_tag="ACHURS_001741"
     CDS             15186..15401
                     /gene="bdm"
                     /locus_tag="ACHURS_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFR1237498.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEQGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            15503..15640
                     /gene="sra"
                     /locus_tag="ACHURS_001742"
     CDS             15503..15640
                     /gene="sra"
                     /locus_tag="ACHURS_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFR1237499.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            15797..17494
                     /gene="maeA"
                     /locus_tag="ACHURS_001743"
     CDS             15797..17494
                     /gene="maeA"
                     /locus_tag="ACHURS_001743"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFR1237500.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            17628..18630
                     /gene="adhP"
                     /locus_tag="ACHURS_001744"
                     /pseudo
     CDS             17628..18630
                     /gene="adhP"
                     /locus_tag="ACHURS_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
     gene            18776..19060
                     /locus_tag="ACHURS_001745"
     CDS             18776..19060
                     /locus_tag="ACHURS_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFR1237501.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(19288..19941)
                     /gene="fdnI"
                     /locus_tag="ACHURS_001746"
     CDS             complement(19288..19941)
                     /gene="fdnI"
                     /locus_tag="ACHURS_001746"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFR1237502.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(19934..20818)
                     /gene="fdnH"
                     /locus_tag="ACHURS_001747"
     CDS             complement(19934..20818)
                     /gene="fdnH"
                     /locus_tag="ACHURS_001747"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFR1237503.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEERVAKLKARGYEHAGVYNPKG
                     VGGTHVMYVLHHADQPELYYGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(20831..23878)
                     /gene="fdnG"
                     /locus_tag="ACHURS_001748"
     CDS             complement(20831..23878)
                     /gene="fdnG"
                     /locus_tag="ACHURS_001748"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(23291..23293),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFR1237504.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            24110..24991
                     /gene="yddG"
                     /locus_tag="ACHURS_001749"
     CDS             24110..24991
                     /gene="yddG"
                     /locus_tag="ACHURS_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFR1237505.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         6134 a         6385 c         6358 g         6216 t
ORIGIN      
        1 gacgaaaata ccatcggcgt ggtgccgact ttcggcgtga cctacaccgg taactatgag
       61 ttcccgcaac cgctgcacga tgcactggat aaattccagg ccgacaccgg tatcgacatc
      121 gacatgcaca tcgacgccgc cagcggtggc ttcctggcac cgttcgtcgc cccggatatc
      181 gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg cttcaggcca caaattcggt
      241 ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg aagaagcgct gccgcaggaa
      301 ctggtgttca acgttgacta cctcggtggt cagattggta cttttgccat caacttctcc
      361 cgcccggcgg gtcaggtgat tgcacagtac tatgaattcc tgcgccttgg tcgtgaaggc
      421 tataccaaag tacagaatgc ttcctaccag gtcgctgcct atctggcgga tgagatcgcc
      481 aaactggggc cgtatgagtt catctgtgcc ggtcgcccgg acgaaggcat cccggcggtt
      541 tgcttcaaac tgaaagaggg tgaagatccg ggatacaccc tgtacgacct ctctgaacgt
      601 ctgcgtctgc gcggctggca ggttccggcc ttcactctcg gcggtgaagc caccgacatc
      661 gtggtgatgc gcattatgtg tcgtcgcggc ttcgaaatgg actttgctga actgttgctg
      721 gaagactaca aagcctccct gaaatatctc agcgatcacc cgaaactgca gggtattgcc
      781 cagcagaaca gctttaaaca tacctgataa cgtttaacgg taacggtgtc ccgaaacgaa
      841 cccgtttcgg gataatttcc aaagtctgtt cactggcatt agcaacggaa aatattgttc
      901 tgaatacgct tcagaacaaa acaggtgcgg ttccgacagg aataccgttt tagggggata
      961 atatggctac atcagtacag acaggtaaag ctaagcagct cacattactc ggcttttttg
     1021 ccataacggc atcgatggta atggctgttt atgaataccc taccttcgca acatcgggct
     1081 tttcattagt cttcttcctg ctattaggcg ggattttatg gtttattccc gtgggacttt
     1141 gtgctgcgga aatggccacc gtcgacggct gggaagaagg tggtgtcttc gcctgggtat
     1201 caaatactct ggggccgaga tggggatttg cagcgatctc atttggctat ctgcaaatcg
     1261 ccattggttt tattccgatg ctctatttcg tgttaggggc actctcctac atcctgaaat
     1321 ggccagcgct gaatgaagac cccattacca aaactattgc agcactcatc attctttggg
     1381 cgctggcatt aacgcagttt ggtggcacga aatacacggc acgaattgct aaagttggct
     1441 tcttcgccgg tatcctgtta cctgcattta ttttgatcgc attagcggct atttatctgc
     1501 actccggtgc ccccgttgct atcgaaatgg attcaaagac cttcttccct gacttctcta
     1561 aagtgggcac cctggttgta tttgttgcct tcattttgag ttatatgggc gtagaagcat
     1621 ccgcaaccca cgtcaatgaa atgagcaacc cagggcgcga ctatccgttg gctatgttac
     1681 tgctgatggt agcggcaatc tgcttaagct ctgttggtgg tttgtctatt gcgatggtca
     1741 ttccgggtaa tgaaatcaac ctctccgcag gggtaatgca aacctttacc gttctgatgt
     1801 cccatgtggc accggaaatt gagtggacgg ttcgcgtgat ctccgcactg ctgttgctgg
     1861 gtgttctggc ggaaatcgcc tcctggattg ttggtccttc tcgcgggatg tatgtcacag
     1921 cgcagaaaaa cctgctgcca gcggcattcg ctaaaatgaa caaaaatggc gtaccggtaa
     1981 cgctggtcat ttcacagctg gtgattactt ctatcgcgtt gatcatcctc accaataccg
     2041 gtggcggtaa caacatgtcc ttcctgatcg cactggcgct gacggtggtg atttatctgt
     2101 gtgcttattt catgctgttt attggctaca ttgtgttggt tcttaaacat cctgacttaa
     2161 aacgcacatt taatatccct ggtggtaaag gggtgaaact ggtcgtggct attgtcggtc
     2221 tgctgacttc aattatggcg tttattgttt ccttcctgcc gccggataac atccagggtg
     2281 actctaccga tatgtatgtt gaattactgg tcgttagttt cctggtggta cttgccctgc
     2341 cctttattct ctatgctgtt catgatcgta aaggcaaagc gaataccggc gtcactctgg
     2401 agccaatcaa cagtcagaac gcaccaaaag gtcacttctt cctgcacccg cgtgcacgtt
     2461 caccacacta tattgtgatg aatgacaaga aacactaacc atgtctgaat ggttataaga
     2521 caagggagcg ataattcatc gctccctttt tcgtgctcgc tgtcgtattg acttaaccgg
     2581 gataagtacg agaatgagcg cacatctgtt taccggaaac cagcatatat ggatatctgc
     2641 tcccgaaacg agaaattagc gattagaaga ccagcgatac tggttgcact ggcactttta
     2701 ctgtgtagtt gtaaaagcac gcctccagag tccatggtga caccaccagc aggttcaaag
     2761 ccaccagcca cgacgcaaca atcgtcacaa ccgatgcgtg gcatctggct ggccacggtg
     2821 tctcgcctcg actggccacc ggtttcctcg gttaacatta gtaaccccac cagccgggcc
     2881 cgtgtacaac aacaggcgat gatcgacaaa ctggatcatc tgcaacgtct cggtataaac
     2941 acggtctttt tccaggttaa gccggacggt accgccctgt ggccatcgaa aattttgcca
     3001 tggtccgatc ttatgaccgg taagattggt gaaaatccgg gttacgatcc gctgcaattt
     3061 atgctcgacg aagcccacaa gcgtgggatg aaagtacacg cctggtttaa cccctatcgc
     3121 gtatcggtta atacgaagcc cggtactatc agggaactga atagcactct gtctcaacaa
     3181 ccggcgagcg tctatgtgca acaccgtgac tggatcagaa cgtctggcga tcgctttgtc
     3241 ctcgacccgg gcatccctga ggttcaggac tggatcacat caatagtcgc agaagtggtt
     3301 tcccgctatc cggtagatgg cgtgcagttt gacgactatt tctatactga gtcaccgggt
     3361 tcacggctaa atgataacga aacgtaccgc aaatacggag gcgcatttgc gtcaaaagca
     3421 gactggcggc gcaacaatac tcagcagtta attgcaaaag tatcgcacac cattaaaagc
     3481 attaagccgg gagtcgaatt tggcgttagc ccggcaggcg tgtggcgtaa ccgatcacac
     3541 gatccgctcg gttccgatac ccgaggcgcg gcagcctatg acgaatccta cgctgacacc
     3601 cgtcgatggg tggaacaagg attgctggat tacattgctc cccaaattta ctggccgttc
     3661 tcacggagtg ccgcgcgtta tgacgtgttg gcaaaatggt gggcggatgt cgttaaaccg
     3721 accaggaccc gcctgtatat cggtatcgcc ttctataaag tgggtgaacc ttcaaagata
     3781 gagccagact ggatgattaa cggcggcgta ccggaactga aaaagcagct cgatcttaac
     3841 gatgcggtac cagaaattag cggcaccatc ttgttccgtg aggactatct gaataaaccg
     3901 cagactcaac aagcggtcag ctatctgcaa agtcgttggg gcagttaaac tcccatgctt
     3961 actttgtaac aagccggtgc tttacccacc ggcttgttac accttgtgaa atatcccact
     4021 agtaatcatg cttacataag tcaaattaac cacgcaatta accacactta gttaaacagc
     4081 tataaatccc taataaaaat gtatgaatat gaaaaaccat acgatattcg ctgtcaccga
     4141 aaaataatta cgatgggcta tactcttctg aagaacagta gcgtagattg aaactgtcct
     4201 aagtcgctta tttcttttca gtatatcttc atatttcagg agaatatggt gcagttagtc
     4261 aatctcgcac gttgcgtttt gattccgtct gatttcccag ccttataagg gtgtgaatta
     4321 tggagatgta ttttaaaaga atgaaagatg agtggaccgg acttgtcgaa caggcagatc
     4381 cactcattcg tgctaaagcc gcggaaattg ccgttgcgca tgctcattat ctgagtatcg
     4441 agttttatcg aattgtccgc atcgacccgc atgccgaaga attcttgagt aatgaacaag
     4501 ttgagcggca gttgaaaagt gcgatggaac gctggattat taacgtgctt tctgcccagg
     4561 ttgacgatgt cgaaaggcta atacaaatcc agcataccgt cgcggaagtg catgcccgca
     4621 taggaattcc ggtagaaatt gtcgagatgg ggtttcgggt gctgaaaaaa atcctctatc
     4681 cggtaatctt ctcttcggat tattccgccg cagaaaaact tcaggtctat catttctcga
     4741 ttaacagtat tgatatcgcg atggaagtga tgacccgcgc gtttaccttt agtgacagta
     4801 gtgcctcaaa ggaagatgaa aattatcgta tcttctcgtt actggaaaac gccgaagaag
     4861 aaaaagaacg gcaaatagcc tcaatacttt catgggaaat agatattatc tataaaatcc
     4921 tgctggattc tgatttaggc agtagtttgc cattaagcca ggctgatttt ggcctgtggt
     4981 ttaaccataa aggtcgacat tattttagtg gcattgccga agtaggccat atctcccgtc
     5041 tcattcagga tttcgacggt attttcaatc aaaccatgcg taacaccagg aatttgaata
     5101 acagaagtct gcgggtgaaa tttttattac agataagaaa taccgtatcg cagattatta
     5161 ccttgctgcg tgaattgttt gaagaagtat cgcgccacga agtcggtatg gatgtactaa
     5221 cgaaattact taaccgccgt ttcctgccga ctatcttcaa acgcgaaatt gcccatgcca
     5281 accggaccgg tacaccgctg tcagtgctga ttattgacgt tgataaattc aaagagatca
     5341 acgatacgtg gggccataac actggcgatg aaattctgcg taaagtctct caggcctttt
     5401 atgacaacgt ccacagtagt gattatgttt ttcgctacgg gggcgatgaa tttatcattg
     5461 ttttgactga agcttctgaa aacgaaacgt tacgtaccgc agaacgtatt cgcagtcggg
     5521 tggagaaaac caaactgaaa gccgcaaacg gcgaggatat tgccctctca ctttccatcg
     5581 gtgccgccat gtttaatggt catcctgact atgagcgcct cattcaaata gccgatgaag
     5641 ctctgtatat cgccaaaaga cgaggtagaa accgtgttga actctggaaa gccagtcttt
     5701 agatgcgcca ggatgcagag gtaatcatga agctaaccga tgcggaaaat gccgccgatg
     5761 gcattttttt ccccgccctt gaacaaaata tgatgggcgc ggtgttaatt aacgaaaatg
     5821 atgaagtgat gtttttcaac cccgccgcag agaagctctg ggggtacaaa cgtgaagaag
     5881 tcattggcaa taacattgat atgctgattc cgcgggattt gcgtcctgcg catcctgaat
     5941 acattcgtca taaccgtgaa ggtggtaaag cgcgcgtcga ggggatgagt cgggagctgc
     6001 actggagaaa aaagacggca gtaaaatctg gacccgtttt gcgctatcga aagtgagcgc
     6061 cgaggggaaa gtttattacc tggcgctggt acgggatgcc agcgtagaaa tggcgcaaaa
     6121 agaacagacc cgacaattga ttattgccgt tgaccatctc gaccgaccgg tgattgtcct
     6181 cgatccggaa cgccatattg tgcagtgcaa tcgcgcattt accgaaatgt ttggttactg
     6241 cattagcgaa gccagcggta tgcagcccga tacactcctg aacattcctg aattccctgc
     6301 cgataaccgc attcgtttac aacagttgct atggaaaacc gcccgcgatc aggacgaatt
     6361 tctgctgttg acacgcaccg gtgaaaaaat ctggattaaa gcctctatca gcccggttta
     6421 tgacgtgctc gcgcatctgc agaacctggt aatgaccttc tcggatatca ccgaagaacg
     6481 gcagatccgc cagcttgaag gcaatattct cgccgccatg tgcagcagcc cgccatttca
     6541 tgaaatgggg gaaatcattt gtcgtaacat cgaatctgtt ctcaacgaat cgcatgtttc
     6601 gctgttcgca ctgcgcaacg ggatgccgat acactgggcg tcatcttcgc acggtgcaga
     6661 agttcaaaat gcgcaaagct ggtcagcgac cattcgtcag cgtgatggtg cgcctgcggg
     6721 gatcctgcaa attaaaacct cgtcaggagc agaaaccagc gcctttatcg aacgcgtggc
     6781 agatatcagc cagcatatgg ccgcgctggc gctggaacag gaaaaaagcc gtcagcatat
     6841 tgaacaactc atccaatttg atccgatgac cggtctgcca aatcgcaata acctgcacaa
     6901 ttacctcgat gaccttgtcg acaaagccgt ctctccggtg gtgtatctca tcggtgttga
     6961 ccatattcag gatgtgattg atagccttgg ctatgcgtgg gccgatcagg cattgctgga
     7021 agtggtcaat cgctttcgtg aaaaactcaa accggatcag tatctctgtc gtatcgaagg
     7081 tacgcagttt gtcctcgtga gcctggaaaa cgacgtcagt aacattaccc aaatcgccga
     7141 tgagctacgg aatgtggtca gcaagccgat aatgattgac gataaaccat tcccgctaac
     7201 cttgagtatt ggcatcagct acgacgtggg taaaaaccgc gattacctgc tctccactgc
     7261 tcacaatgca atggattata ttcgcaagaa tggcggtaac ggctggcagt tcttcagccc
     7321 ggcgatgaac gaaatggtaa aagagcgttt ggttttaggc gcagcgctga aagaagcgat
     7381 tagcaataac caactgaaac tggtttacca gccgcaaatc ttcgcagaaa cgggtgaact
     7441 gtacggcatc gaagcccttg ctcgctggca cgatccccag catggtcatg tacccccttc
     7501 acggtttatt cctctcgcag aagagattgg tgaaatcgaa aatattgggc gctgggtcat
     7561 cgcggaagct tgccgtcagt tagcagaatg gcgtagccag aatattcata tcccggcgtt
     7621 atccgtgaac ttgtcggcgc tgcactttcg cagtaatcag ctgcctaatc aggtgtctga
     7681 tgcaatgcaa gcctggggta ttgacggcca ccagctgacg gtagaaatca cggaaagcat
     7741 gatgatggaa cacgataccg aaatctttaa gcgcattcag atcctgcgcg atatgggcgt
     7801 gggcttatcg gtagatgatt ttggcacggg cttttccgga ttatcccgct tagtcagtct
     7861 tccggtaacg gaaatcaaaa ttgacaaaag ttttgtcgat cgttgtctga cggaaaaacg
     7921 catccttgcc ttacttgaag ccattaccag cattgggcaa agcctcaatt taaccgtcgt
     7981 ggcggaaggc gtcgaaacca aagagcaatt tgagatgcta cgcaagatcc actgtcgcgt
     8041 tattcaggga tatttctttt cccgccccct acccgccgag gaaattccag gctggatgag
     8101 cagcgtgtta ccgctgaaaa tctgacaaat tcctctcgcc cgcactcgcg ggttttcatt
     8161 taacgtgaca ctgtcactta aatactgtga tttcagctac gattccggga gattccttcc
     8221 ttaacttccc ctcttgcacc aaaactgcac tacgctgcac aaatgttgat caaaagttaa
     8281 aaaatataac tttaagccag cattctggcg cttatcccgg catggcatga gatctgcata
     8341 agcggaaagc gcagcaattt ttgtcttaat acaggaaccg tttatgtcgg ataccaccga
     8401 actggttgat ttagccgtga tcttccctga tctggagatc gaattgaaat acgcctgcgc
     8461 tgataacatc acaggtaaag ctatttacca gcaagcgcgt tgtctgttac acaaggatgc
     8521 gattaccgcg ctggcgaaaa gtatcagtat cgcccagctg tcaggcttac aactggtgat
     8581 ttacgatgcg tatcgcccac aacaagcaca ggcgatgttg tggcaagcct gcccagaccc
     8641 gcaatatgtt gttgatgtga cggtcggttc taatcacagc cgtggcacgg cgatcgacct
     8701 gacgcttcgt gatgagcacg ggaacatcct ggatatgggc gcagggttcg atgaaatgca
     8761 cgagcgctcc catgcctatc acccttccgt cccgcccgcc gctcagcgca atcgcctgtt
     8821 gctgaatgcg ataatgactg gtggcggttt tgtcggtatc tccagcgaat ggtggcactt
     8881 cgaattaccc caggcagcga gttaccctct gctcgctgat caattctctt gttttatatc
     8941 ccctggcaca cagcacgtca gttaatcagg aaactgtcat gaagagatcg atatcgtttc
     9001 gtcccacatt gctcgcgctc gtccttgcca caactttccc ggttgcgcac gccgccgtac
     9061 cgaaagatat gctggtgatt ggtaaggccg ccgatccaca aaccctcgac ccggcggtaa
     9121 caatagataa taacgactgg acagtgacct acccgtctta tcagcggctg gttcagtaca
     9181 aaacggacgg tgataaaggc tcaaccgacg ttgaaggcga tctggcaagt agctggaaag
     9241 cgtctgacga tcaaaaagag tggacgttca ccctgaagga caatgctaaa tttgccgatg
     9301 gcacacctgt cactgccgaa gcagtaaaac tctcttttga gcggttacta aaaatcggcc
     9361 aggggccagc agaagcattt cccaaagatt taaagattga tactcccgac gaacatacgg
     9421 tgaagtttac ccttagccaa ccattcgcac cgttcctcta cacgctagcg aatgacggtg
     9481 catccattat caatccggcg gtcttaaagg aacatgcagc ggatgatgct cgcggcttcc
     9541 tcgcgcaaaa taccgccggt tccggaccat ttatgctgaa aagctggcaa aaaggtcagc
     9601 aattagttct ggtgccaaat ccgcattacc ccggcaataa accgaacttt aagcgagtat
     9661 cggtaaaaat tatcggtgaa agtgcctccc gtcgcctgca gctctcccgt ggcgacattg
     9721 acattgccga tgcgctgccg gtggatcaac tcaacgccct gaagcaggaa aataaagtca
     9781 atgtggcaga gtatccgtca ctgcgcgtta cctatctgta tctgaataac agcaaagcgc
     9841 ctcttaatca ggcggatctg cgtcgggcca tttcctggtc taccgattat cagggcatgg
     9901 ttaacggcat tctgagtggt aacggaaaac agatgcgcgg cccgattccg gaaggcatgt
     9961 ggggctacga tgcgacggca atgcaataca accatgacga aacgaaagcc aaagccgaat
    10021 gggataaagt gacgagcaaa cccaccagcc tgacgtttct ctactccgat aacgatccga
    10081 actgggagcc tattgctctg gcgacacaat ccagtctcaa caagctgggc atcaatgtga
    10141 agctggaaaa gctggcgaac gccaccatgc gcgacagagt gggtaaaggt gattacgaca
    10201 ttgcgattgg caactggagt ccggattttg ccgacccgta tatgtttatg aattactggt
    10261 ttgagtcaga caaaaaaggt ctgccgggta accgctcgtt ctatgaaaac agtgaggtcg
    10321 ataagttact gcgcaatgcg cttgcgacca ccgaccagac gcagcgtacc cgggactacc
    10381 agcaggcaca gaaaatcgtc attgatgacg ctgcttatgt gtacctgttc cagaaaaact
    10441 accaactggc gatgaacaaa gaggtgaaag gctttgtgtt caatcccatg ctggaacagg
    10501 tcttcaatat caataccatg agtaaataac gtgaccttct ggagtatttt acgccaacgc
    10561 tgctgggggc tggtgctcgt ggtggcgggc gtctgcgtga ttacgtttat tatctcgcat
    10621 ctgatccctg gcgatccggc gcggttactg gcgggtgacc gcgccagcga tgctatcgtg
    10681 gaaaatattc gccagcaact gggactggac cagccactgt atgtacagtt ttaccgctac
    10741 ttcagcgacc tgtttcatgg tgacctggga acatccattc gtaccgggcg tccggtgctg
    10801 gaagagttgc gtatattttt cccggcgacg ctggaactgg ctttttgtgc cctgctgctg
    10861 gcactcctga ttggcatccc gctgggcata ctctctgcag tctggcgaaa tcgctggctg
    10921 gatcatctgg tgcgaataat ggccattacc ggaatctcca cacctgcgtt ctggcttgga
    10981 ctgggcgtca ttgtgctgtt ttatggtcat ctgcaaattc ttcccggcgg cggaaggctt
    11041 gatgactggc tggatccacc aacgcacgtt accggctttt atctgctcga tgcgctgctt
    11101 gaaggcaacg gtgaagtctt cttcaatgcg ttgcaacatc tcatcttacc ggcattaacg
    11161 ctggcgttcg ttcacctggg aattgtcgct cgccagatcc gctcagcgat gctggaacaa
    11221 ttgagtgaag actacattcg taccgcccgg gccagcggct tgcccggctg gtatatcgtt
    11281 ttatgttatg cgctacccaa tgcgttgatc ccatcgatta ccgtattggg tctggcgctg
    11341 ggcgatttgt tgtatggcgc agtgctcacc gaaaccgttt ttgcctggcc cggaatgggt
    11401 gcatgggtag taacatcaat acaggcgctc gacttcccgg cagtgatggg ctttgccgtc
    11461 gtggtttcat ttgcttatgt gctggtcaac ctggtggtgg atttgctcta tttgtggatt
    11521 gatccgcgta tcggacgtgg aggtggtgaa tgatgctaag cgaggaaacg tccgccgtac
    11581 gcccacaaaa acaaacgcga tttaacggtg caaaactggt atggatgctg aaaggcagtc
    11641 cgctcaccgt gaccggcgca gtcatcattg tattaatgct attgatgatg attttttcac
    11701 cgtggcttgc gacgcatgat cccaacgcca ttgatttaac cgcccgcctt ttgccgcctt
    11761 ctgcggcgca ctggtttggc accgatgaag tgggacgcga tctgttcagc cgcgtactgg
    11821 tcggcagtca gcaatcaatt ctcgccggat tagtcgtggt tgccattgcg ggtatgattg
    11881 gttcgctact cggatgtcta tccggtgtgc ttggcggacg cgcagacgcc atcatcatgc
    11941 gcatcatgga cattatgctg tcgattcctt cgttggtact gacaatggca ctggctgccg
    12001 ctctcgggcc gagtttattt aacgccatgc tggcgattgc tattgtgcga attccctttt
    12061 atgtgcgcct ggcgcggggg caagcattag ttgtacgcca gtatacctat gttcaggcgg
    12121 cgaaaacctt tggtgcgtcg cgttggcatc tgatcagctg gcatatttta cgtaactccc
    12181 tgccgccgct gatcgtgcag gcatcgctgg atatcggtag cgcgatttta atggccgcca
    12241 cgttgggatt tattggcctg ggtgctcaac aaccgagtgc tgaatggggc gcgatggtag
    12301 ctaatggtcg caactatgta ctcgatcagt ggtggtattg cgcatttccg ggggcagcga
    12361 ttttgcttac cgccgtcggg tttaatctct ttggtgatgg tattcgcgat ctgcttgacc
    12421 cgaaagcagg aggaaagcag tcatgaccca acccgttctg gacattcaac agctgcattt
    12481 gagtttcccc ggttttaacg gttatgttca cgtgctcaac aatgtgtcct tgcagattaa
    12541 ccgcggtgaa attgtcggtc tggtgggaga atccggctca ggtaaatcag tcaccgcaat
    12601 gctgattatg cgtctgctac cgacgggcag ttattgcgta catcgggggc agatttcact
    12661 gctgggagac gatgttctta acgctcggga gaaacagctt cgtcagtggc gcggcgcacg
    12721 agtggcgatg atctttcagg aaccgatgac cgccctcaat ccgacacgtc gaataggtct
    12781 tcagatgatg gacgtgatcc gccatcatca accaataagt cgtcgggaag ccagagctaa
    12841 agcgattacc ctgctggaag agatgcaaat cccggatgcc gtggaagtta tgtcgcgcta
    12901 tccgtttgag ctttcaggtg gtatgcgcca gcgggtaatg attgcgctgg cattctcctg
    12961 cgagccgcaa ttgattattg ccgacgaacc gactacggcg ctggacgtca cggtacagtt
    13021 gcaggtactg cgtttgctta aacataaagc tcgcgccagt ggaactgcgg tactgttcat
    13081 cagccatgat atggccgtgg tgtcgcaact gtgcgatagc gtttacgtga tgtatgccgg
    13141 aagcgtaatt gaaagcggcg tgacggcaga cgttatccat catccccggc atccgtatac
    13201 cattggtttg ctgcaatgcg caccagaaca tggagtacca cgccagccat tacccgccat
    13261 tccagggacg gtaccaaacc tcacccattt gcctgacggc tgcgcttttc gcgatcgttg
    13321 ctatgcggca ggtgcacagt gtgaaaacgt cccggcgctg acagcgtgtg gtgacaacaa
    13381 ccagcgctgc gcctgttggt atccgcagca ggaggtcata agtatctgac acgttattaa
    13441 cgttacgcga cgtccatatc aatttcccgg cccgtaaaag ctggcttggt aaaactacgg
    13501 aacatgttca tgcgattaat ggtattgatt tacagatccg ccgtggtgaa accttaggga
    13561 tcgtcggtga gtcaggctgc ggcaaaagca ccctcgcaca gcttttaatg ggtatgctgc
    13621 aaccgagcca cgggcaatac atccgttcag gatcacaacg cattatgcag atggtgtttc
    13681 aggacccgct ctcttcgctt aatccgcgct tatcggtgtg gcgcatcatc acagaaccgc
    13741 tctggatagc taagcgtagt agtgaacaac agcggcgagc gttggcaaag gagttggctg
    13801 tgcaggtggg tattcgtccg gagtatctcg accgcctgcc tcatgcgttc tccggcgggc
    13861 agcggcaacg catcgccatt gccattgcca gagcactctc ttcgcagcct gacgtgattg
    13921 tgcttgatga gccaacctcg gcgctggata tctccgtgca ggcgcagatc ctcaatttac
    13981 tggtaacgct acaggaaaat cacgggctga cttatgtgct gatttcacac aatgtctcgg
    14041 tgatacgtca tatgagcgat cgggtggcgg tgatgtatct cgggcagatt gtagaactgg
    14101 gcgacgcgca gcaggtgctg acggcacctg cacatccata cacccgatta ttgctggatt
    14161 ccctccccgc cattgataaa ccgctggagg aagaatgggc attatgtaaa acggatctgc
    14221 cgggaaaccg cacgttgcca caaggctgtt ttttctacga acgttgccct ctggcaaccc
    14281 acggatgtga agtccggcaa tcattagcaa taagggagga cggacgtgag ctccggtgct
    14341 ggcgggcgct gtagagatgc taacaacgca tcaggcattc acgcgcctga cgcgtcatcc
    14401 ggcaatgctt tacgatttca actggtaatc cagcgtaatt tccgctttca gtacctgaga
    14461 aaccgggcat cctgcttttg ctttctggat gatgccgtca aaggtagagg catcaatacc
    14521 cggcaccgca acttcactct tcagtgcgat tttcgtaatc gcaaaatcgg catccacttt
    14581 atccagcgac acatcggcgg tggtatcaat cgatgttggc gtgaatcccg cttcccccag
    14641 cattaatgaa agcgccattg agaaacatgc ggcatgcgct gcgccaatca gttcttcagg
    14701 gttggttcct ttttcgcctt caaaacgcgt gttaaatcca tacggctgtt ggttcagcac
    14761 gccactctgg gtggatactg ttcccttccc gcgtttgata tcgccttccc agtgtgcctg
    14821 acctttctta tggattgtca ttgttgctct cctgtgggct taaaaaggaa attatagact
    14881 acgtgagaaa cagcacgagg ataagcagga tattccgaat aaaatacggc gaaaatatta
    14941 cttctgcact taaattaaat tcccacaata gataaccggg aatatatatc tcaagagata
    15001 ttgcatcaat atcaatcaac tcaccacaat aataatacat atcaatatca tggagttaaa
    15061 atataaaaat catcaaccag gactaatctt aacaacgaag cggcaaatat ttgccatgct
    15121 gaatgtgctt tataaatctg cgatccgtag cagacaccat aaatacacag acacggagaa
    15181 tcactatgtt tacttattat caggcagaaa attcaacagc agaacccgct ctggttaatg
    15241 ccattgagca aggccttcgg gcagagcaag gtgttgtcac tgaagatgac attctcatgg
    15301 agctgaccaa atgggtggaa gcttctgata acgacatcct cagtgatatc taccagcaaa
    15361 cgatcaatta tgtggtcagt ggccagcacc ctacgcttta aggtgctatg cttgatcggc
    15421 aacctaattt aggggtttag cacgtgtttc ttcgctacgg cgatgttgtc cttaaaacta
    15481 gcaacaggat tgaggagtta aaatgaaatc gaaccgtcag gcacgtcata ttcttggact
    15541 ggaccataaa atttctaacc agcgcaaaat agttaccgaa ggtgacaaat ccagcgtggt
    15601 aaataaccca accggcagaa aacgccccgc tgaaaagtaa ttcataacca tcagtcctca
    15661 atgacgatta aacaccattg cctgcgcaat ggtgtttttg tttttatctg ctttatactt
    15721 ggggccgacg ccctggcggt aaagcaaaga cgataaaagc gtgccaggga tggatattca
    15781 aaaaagagtg agtgacatgg aaccaaaaac aaaaaaacag cgttcgcttt atatccctta
    15841 cgctggccct gtactgctgg aatttccgtt gttgaataaa ggcagtgcct tcagcatgga
    15901 agaacgccgt aacttcaacc tgctggggtt actgccggaa gtggtcgaaa ccatcgaaga
    15961 acaagcggaa cgagcatgga tccagtatca gggattcaaa accgaaatcg acaaacacat
    16021 ctacctgcgt aacatccagg acaccaacga aaccctcttc taccgtctgg taaacaatca
    16081 tcttgatgag atgatgcctg ttatttatac cccaaccgtc ggcgcagcct gtgagcgttt
    16141 ttctgagatc taccgccgtt cacgcggcgt gtttatctct taccagaacc gccacaatat
    16201 ggacgatatt ctgcaaaacg taccgaacca taatatcaaa gtgattgtgg tgactgacgg
    16261 tgaacgtatt ctggggcttg gtgaccaggg catcggcggg atgggcattc cgatcggtaa
    16321 actgtcgctc tataccgcct gtggcggcat cagcccggcg tatacccttc cggtggtgct
    16381 ggatgtcgga acgaacaacc aacaactgct taacgatccg ctgtatatgg gctggcgtaa
    16441 tccgcgtatc actgacgacg aatactatga attcgttgat gaatttatcc aggctgtgaa
    16501 acaacgctgg ccagacgtgc tgttgcagtt tgaagacttt gctcaaaaaa atgcgatgcc
    16561 gttacttaac cgctatcgca atgaaatttg ttcttttaac gatgacattc agggcactgc
    16621 ggcggtaaca gtcggcacac tgatcgcagc aagccgagcg gcaggtggtc agttaagcga
    16681 gaaaaaaatc gtcttccttg gcgcaggttc agcgggatgc ggcattgccg aaatgatcat
    16741 cgcccagacc cagcgcgaag gattaagcga ggaagcggcg cggcagaaag tctttatggt
    16801 cgatcgcttt ggcctgctga cggacaagat gccgaacctg ctgcctttcc agaccaaact
    16861 ggtgcagaaa cgcgaaaacc tcagtgactg ggataccgac agcgatgtgc tgtcactgct
    16921 ggatgtggtg cgcaatgtaa aaccagatat tctgattggc gtctcaggac agaccgggct
    16981 gtttacggaa gagatcatcc gtgagatgca taaacactgt ccgcgtccga tcgtgatgcc
    17041 gctgtctaac ccgacgtcac gcgtggaagc cacaccgcag gacattatcg cctggaccga
    17101 aggtaacgcg ctggtcgcca cgggcagccc gtttaatcca gtggtatgga aagataaaat
    17161 ctaccctatc gcccagtgta acaacgcctt tattttcccg ggcatcggcc tgggggttat
    17221 tgcttccggc gcgtcacgta tcaccgatga gatgctgatg tcggcaagtg aaacgcttgc
    17281 acagtattcg ccgctggtgc tgaacggcga aggtctggta ctaccggaac taaaagatat
    17341 tcagaaagtc tcccgtgcaa ttgcgtttgc ggttggcaaa atggcgcagc agcaaggcgt
    17401 ggcggtgaaa acctctgccg aagctttgca acaagccatt gacgataatt tctggcaagc
    17461 cgaataccgc gactaccgcc gtacctccat ctaagcctgc gcccggtagt gaaggctacc
    17521 gggcaatttt cctctccctt tttcagatct catccatact gggtagtggc gaataaatct
    17581 catttgcctc acctgctatg cagaacatca tccgaaaagg aggaactatg aaggctgcag
    17641 ttgttacgaa ggatcatcat gttgacgtta cggataaaac actgcgctca ctgaaacatg
    17701 gcgaagccct gctgaaaatg gagtgttgtg gtgtatgtca taccgatctt catgttaaga
    17761 atggcgattt tggtgacaaa accgacgtaa ttctgggcca tgaaggcatc ggtgtggtgg
    17821 cagaagtggg tccaggtgtc acctcattaa aaccaggcga tcgtgccagc gtggcgtggt
    17881 tctacgaagg atgcggtcat tgcgaatact gtaacagtgg taacgaaacg ctctgccgtt
    17941 cagttaaaaa tgccggatac agcgttgatg gcggaagagt gcatcgtggt cgccgattac
    18001 gcggtaaaag tgccagatgg tctggactcg gcggcggcca gcagcattac ctgtgcgggg
    18061 gtcaccacct acaaagccgt taagctgtca aaaattcgtc ccgggcagtg gattgctatc
    18121 tacggtcttg gcggtctggg taacctcgcc ctgcaatacg caaagaatgt ctttaacgcc
    18181 aaagtgatcg ccattgatgt caatgatgag cagttaaaac tggcaaccga aatgggtgca
    18241 gatttagcga ttaactcacg caccgaagac gccgccaaaa ttgtgcagga gaaaaccggt
    18301 ggcgctcact ctgcggtggt aacagcagta gctaaagctg cgtttaactc ggcagttgat
    18361 gctgtccgtg caggcggtcg tgttgtggct gtcggtctgc cgccggagtc tatgagcctg
    18421 gatatcccac gtcttgtgct ggatggcatt gaagtggtcg gttcgctggt cggcacgcgc
    18481 caggatctaa ccgaagcctt ccagtttgcc gccgaaggca aagtggtgcc gaaagtcgcc
    18541 ctgcgtccgt tagcggacat caacaccatc tttaccgaga tggaagaagg caaaatccgt
    18601 ggccgtatgg tgattgattt ccgtcactaa gaggtctttg ctgcgactcc catgttcggg
    18661 tcgcagcatc gcacactctc caacatgaaa tggctaaatg gattattgtt gcgtaggaag
    18721 gaacaataat gatagtgttc ccagagaagg aacgctactg agagtaaaga cactaatgaa
    18781 aatggccaat catccccgcc cgggggacat tattcaggaa tcactggacg aacttaatgt
    18841 cagcctgcgc gagtttgcca gagcaatgga aattgcgccc tcaacggcaa gccgattgct
    18901 gaccggaaaa gcagctttga cgccagaaat ggcaattaaa ctttccgtgg tgatcggcag
    18961 ttcgccgcaa atgtggctga atctgcaaaa cgcctggagt ctggcagagg cagaaaaaac
    19021 ggtggatgtg tcgcgattac gccgtctggt tacgcaataa aaaatggcac cttataaagc
    19081 gccatttgaa actgatgaca aacgcaacat tgcctgatgc gctacgctta tcaggcctac
    19141 acgttcactt caatctattg tatttgaagg gttttcatgc cggataaggc attcacgacg
    19201 catccgggat gaacaaagcc cacgttgtct acaatatgaa aatggcgcct tttacagcgc
    19261 cattttcagc ttaaagttca aaagagatta tatcccttct tcactctctt ttttcgcttc
    19321 tgccttctcg atttcacgat accagcgcgg atggtgtttc ttcgcccagc gacggctcac
    19381 cttcccttcg atcatccctt taatcgaccc tttcacccag aatgccatat acatatggat
    19441 caggatggcg tggatcagga tgatacccgc agccgcgtgg atcagcaggc tgtagcgaac
    19501 aacctgcatc gggaagtact gcgcaaagta cggacgccag ataatcaccc cggtcaccag
    19561 cagcacgaaa atcatgctca tgatcgacca gaacatcatc ttttgcccgg cgttgtactt
    19621 accaacatcc gccactttgt gctcattgcc ttttaatact tcgacaatgt tcaacagcca
    19681 cggaatatct ttcttatccg ggatgttgtg atgcacaaaa cgcacaaaca taaacatcag
    19741 tgcgacgaaa atcgcaatgc cgaagaacgg gtgcaaaatg cgtcccatct gcggcgtacc
    19801 gaaggtttgc gtcagccatt gcagcgtcgg gaagaagaac gaaatcccgg acagcgccac
    19861 caggaagaag caaatcacca cggtccagtg acaggcgcga tcaataaatt tggtgcgcac
    19921 aatcattttc gacttactca tgatgatcct cctcgtcatc gtccacttcc ttattcgggc
    19981 caataccgat gtagtggaaa atcaacccgg caaaagtggc aataaagcca gccgctgcca
    20041 gcggtttcaa cgcgcctttc cacaggctta ccgaggtgtc gatcttcgga tctttcggca
    20101 gaccgtaata cagctccggc tgatcggcgt gatgcagcac gtacataacg tgcgtaccac
    20161 cgaccccttt cgggttgtag acgccagcat gttcgtaacc acgcgctttc agtttcgcca
    20221 cgcgctcttc cgccagctcc agcatctcct tcttggtgcc gaagtggata gccccggtcg
    20281 gacaggtttt cacacaagcc ggttcctggc cgacgctgac gcgatcgacg cagagcgtgc
    20341 atttatatac ccggttatcc tctttgttga ggcgcggaat attaaacgga cacccggcaa
    20401 tgcagtaacc acagccgatg cagttttccg actggaaatc gacaatcccg ttagcgtact
    20461 gaatgattgc accagcagac gggcacgcct tcaggcagcc gggatcttca cagtgcatac
    20521 agccgtcttt acggatcagc cactccagct tgccgttctg ttcggtttcg ctaaagcgca
    20581 tcaccgtcca ggacttggcg ctcagatcgg cggggttatc gtaaaccccg acgcagtgcc
    20641 ccacttcatc acggatgtcg ttccactccg aacacgccac ctgacaggct ttacagccga
    20701 tacaggtgga aacgtcgata agttttgcga cttctgcttt gtaatcacgc acctgagaag
    20761 gcggcgtgat ggagttagtt gcggaccttt tgataatatc ctgcgtttcc atagccatct
    20821 gttcgccccc ttacgccttc tcgatgttga ctaagaacgc tttatattcc ggcgtttgcg
    20881 agtttgcatc accgacattc ggcgtcagag tgttagcgat ataaccttta cgcgcgacac
    20941 cctcaaagcc ccagtggatt ggaataccca ccgtttcaac ctgctgaccg tttacattca
    21001 gcggtttcag acggcgcgtt accaccgcca ccgcgcggat aaagccacgc ttgcttgaga
    21061 cagtgacacg atcgccatta ttaatgcctt tcgccgccgc cagcgtttcg ctgatttcca
    21121 caaactgttc cggctgagca attgcgttga gcaatgcgtg cttggtccag gtgtggaagt
    21181 gctcggtcag acgataggtc gtacccacat acgggaactg ctctttttta cccatccgca
    21241 gcgcgtcttg ttcatacaga cgaacaaccg ggttagacac cacgttcgga tgcagcgggt
    21301 tagtgcccag cggcgtttca atcggctcgt agtgttccgg gaacggacct tccgccattt
    21361 tgttgatggc aaacaggcgt cccatccctt ccggctgcat gataaacggc ccggttggcg
    21421 tacccggtgc ggcattgccg aagtccggaa tatcgttacc cgtccacttg ctgccgttcc
    21481 actggatcag catccgtttc ggatcccacg gtttaccatt gatatccgcc gaagcacggt
    21541 tgtagagcac gcgacggttg agcggccacg cccaggccca tcccagcgta ttccccagac
    21601 cggatgggtc ggagttatcg cggttagcca tctggttgcc ctgctctgtc cagctaccgg
    21661 tgtagatcca gcaagaagat gcggttgtac cgtcatcacg cagatgcgca aagctactca
    21721 gcaactgacc tttcttcgca atcagcacgc cattagcgtc atagagatct tccagcgcat
    21781 agccgttatt ctctttcgcc acttcgtcag attgcggttc gtgcggctgc ttgtagttcc
    21841 agctcatctt catcagcggt tctacgcctt taccaccttc ggcctggtac agctcgcgca
    21901 gatgatggta gatacccgcc agaatttcgc cgtcgttacg cgcttcgccc ggcgcgtcct
    21961 gacctttcca gtgccactgc aaccagcgac cggagttagc gatagaacca tcttcttcgg
    22021 caaagcaggt cgaaggcaga cggaatactt cagtctgaat agacgccgga tcgacatcgt
    22081 tcgactcgcc gtggttctgc cagaaggtag aggtttcagt caccagcgga tcgataacca
    22141 ccatgtactt cagcttgctc aggcagctca ccactttgtt tttgtccggg aaggacgcaa
    22201 ccgggttaaa gccctggcag aagtaaccgg tgactttgcc ttcgtccatc atgttgaaat
    22261 acttgatgac gtcgtaggtc tggtcccact tcggcagcca gtcatagccc cagttgttct
    22321 ctttctgcgc ggcatcgcca tagaaagatt tcatcaggct aacgaagaac ttcggatagt
    22381 tgctccagta gttcacctga tcagccagcg tcgctttcgg cgtgttcgct tccagatacg
    22441 actgcaaatc aacctgtttt tctgacggaa gcgtcagata acctggcagg ctggtagaga
    22501 gcagacctaa gtcagtcagc ccctgaatgt tggagtgacc acgcaatgcg ttcacgccgc
    22561 caccggccat acccatgtta ccgagcagca actggatcat cgccatagta cggatgttct
    22621 gcgcacccac ggtgtgctgc gtccagccca gcgcgtacag gaaggtggtt gtgcgatccg
    22681 gtgcgctggt ggaggccagc acttcacaca ctttcaggaa gtcggctttt ggcgtaccgc
    22741 agatgttttc aacgacgtcc ggcgtgtagc gggaaacgtg cgctttcagc aggttccaca
    22801 cacagcgcgg atgagtcagt gtttcatcgc gttttgcata gccgttttca tcgaactgat
    22861 agttccagga cgacttatca tactggcgtt tttcagcgtc gtagccgctg aacaggccgt
    22921 cttcgaaagc aaaatcatca cgcaccagca ggctggcgtt ggtgtaatgt ttaacgtatt
    22981 cggcgttgat tttgttgttt tcgatcaggt agcgcaaaac gccagacagg aacgtaatgt
    23041 ccgtaccgga acgaataggt gcgtaaatat ccgccacaga agcggtacgc gtaaaacgag
    23101 gatcgacaac gatcaaggtt gcatcgttgt tgtttttcgc ttccatcgcc cagcggaaac
    23161 cgacgggatg cgcttcagca gcgttaccgc ccattaccat tacgacgtta gcgtttttga
    23221 tatccaccca gtggttggtc atcgcaccgc gaccaaatgt tggagcaaga cttgctaccg
    23281 ttggtccgtg tcagacgcgc gcctggttgt ctaccgccag catcccgagg gagcgggcaa
    23341 atttctgggt cagcatcccg gtttcgttgc tggcaccgga ggcacacagc ataccggtag
    23401 aaagccaacg gtttaccgtt acgccctgct cgttcttttc aataaagtta gcgtcacggt
    23461 cagctttcat cagcttcgca atacgggaga atgcttcttc ccagctaatg cgctgccatt
    23521 tgtcagaacc tggcgcacga tattccgggt agcgcagacg gttttcgctg ttgacgtaat
    23581 ccagcaaacc ggcccctttt gggcacagcg caccacggct taccggatga tccgggtcac
    23641 cttcaatgtg ataaatcgct tctctggcgt ttttcgcgcc atcacccagg ctatacatca
    23701 atagcccgca acctacggaa cagtatgtgc aggtgttacg gatctcttta gcgcgtaata
    23761 atttgtagtt tcgcgcctga gccagtgctt gcttcggggc aaagcccaat gccgctaccg
    23821 ttgttccagc cataccgccc gcgcagattt taaaaaattg tctgcgactg acgtccattg
    23881 ctttcctctt ttttcagggg tattactgcg cgaggaaact aacagtaaaa ttctgaatct
    23941 ttttgcgcga aatcaaaaag aacacgtgta tgccacaaaa taatccgcct gcgcaaatta
    24001 acctacctca atagcggtag aaaaacgcac cactgcctga caggccagtt aaaaaaatgc
    24061 tataaaattc agcttaattt ttaacggcaa gagagacaaa acagcgagca tgacacgaca
    24121 aaaagcaacg ctcatagggc taatagcgat cgtcctgtgg agcacgatgg taggattgat
    24181 tcgcggtgtc agcgaggggc tcggtccggt cggcggcgca gctgctatct attcattaag
    24241 cgggctgctg ttaatcttca cggttggatt tccgcgtatt cggcagattc cgaaaggcta
    24301 tttactcgcc gggagtctgt tattcgtcag ctatgaaatc tgtctggcgc tttccttagg
    24361 gtatgcggcg acccgtcatc aggcgattga agtgggtatg gtgaactatc tgtggcccag
    24421 cctgacaatt ctctttgcca ttctgtttaa tggtcagaaa accaactggc tgattgtacc
    24481 aggattatta ttagccctgg tcggcgtctg ctgggtgtta ggcggtgaga atgggttaca
    24541 ttatgatgaa atcatcaata atatcaccac cagcccattg agttatttcc tggcgtttat
    24601 tggtgcgttt atctgggcag cctattgcac agtaacgaat aaatacgcac gcggatttaa
    24661 cggaattacc gtttttgtcc tgctaacggg agcatgtctg tggatttact attttctcac
    24721 gccacaacca gaaatggtat ttagcacgcc cgtcatgatt aaactcatct ctgcggcatt
    24781 taccttagga tttgcttatg ctgcatggaa tgtcggtata ttgcatggca atgtcaccat
    24841 aatggcggta ggctcgtatt ttacgcctgt actttcctca gcgcttgcag ccgtgctgct
    24901 cagcgccccg ctgtcgttct cgttctggca aggcgcgcta atggtctgcg gcggttccct
    24961 gctctgctgg ctggcgacac gtcgtggtta aaataattgc cgggtctacg gcccggcagt
    25021 taatcagata aattccgaca atattaaaaa ataaatttca aataccagta attcacctta
    25081 tatctcattt cga
//
LOCUS       DAZPDU010000047        25054 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZPDU010000047 DAZPDU010000000
VERSION     DAZPDU010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25054
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.047"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..566)
                     /locus_tag="ACHURS_001750"
     CDS             complement(<1..566)
                     /locus_tag="ACHURS_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFR1237506.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRFGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFGVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPALPQKPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRY"
     gene            complement(643..954)
                     /locus_tag="ACHURS_001751"
     CDS             complement(643..954)
                     /locus_tag="ACHURS_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1237507.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASLSPFSDERPSSRFRGIALPSERRRLRYSTVGLTRYRT
                     R"
     gene            complement(951..1877)
                     /locus_tag="ACHURS_001752"
     CDS             complement(951..1877)
                     /locus_tag="ACHURS_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFR1237508.1"
                     /translation="MPNWCSNRMHFSGEPAQIAEIKRLASGAVTPFYRRATNEGIQLF
                     LAGSAGLLQTTEDVQFEPCPGLTAAGRGVVSPENIAFTRWLTHLQNGVLLDEQNCLML
                     HELWLQSGTGQRRWEGLPDDVRESITALFTPKRGDWCDIWSNEDVSVWWNRLCDNVLP
                     EKTMPFDLRMVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQG
                     ENFIQVDFDTPWCQPESDVVAELSRRFGCTLEHWYAEQGCNFCGWQLYERGELVDVLW
                     GELEWSSPTDDDELPEVTGPAWIVDNVTHYGG"
     gene            complement(1948..2184)
                     /locus_tag="ACHURS_001753"
     CDS             complement(1948..2184)
                     /locus_tag="ACHURS_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015589601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1237509.1"
                     /translation="MRRLTRRGRNAACGQCPASGRARDGRFYRSRLASVTVYASPSPF
                     SDERPSSRFRGITLPSNRRRLRYSTVGLTRYRTR"
     gene            2304..2981
                     /gene="tnpA"
                     /locus_tag="ACHURS_001754"
     CDS             2304..2981
                     /gene="tnpA"
                     /locus_tag="ACHURS_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFR1237510.1"
                     /translation="MSIIFNGHYRMKHRTWITEALRLHFEEHLPRVVAGRRLGVPKST
                     VCSMFVRFRRAGLSWPLPAGMSEQELDACLYGQFSTVPVVRPESTVISEAPVVKKRPR
                     RPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYRKGGLLPSGKNMPA
                     LLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRLKGKLTPALLQTLIR
                     EIKGSSH"
     gene            2981..3328
                     /gene="tnpB"
                     /locus_tag="ACHURS_001755"
     CDS             2981..3328
                     /gene="tnpB"
                     /locus_tag="ACHURS_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR1237511.1"
                     /translation="MISLPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTKRLERGRFVWPVTRDGKVHLTPAQLSMLLEGINW
                     KHPKRTERAGIRI"
     gene            3348..4919
                     /locus_tag="ACHURS_001756"
     CDS             3348..4919
                     /locus_tag="ACHURS_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISCro1 family transposase"
                     /protein_id="HFR1237512.1"
                     /translation="MDTSLAHENARLRALLQTQQDTIRQMAEYNRLLSQRVAAYASEI
                     NRLKALVAKLQRMQFGKSSEKLRAKTERQIQEAQERISALQEEMAETLGEQYDPVLPS
                     ALRQSSARKPLPASLPRETRVIRPEEECCPACGGELSSLGCDVSEQLELISSAFKVIE
                     TQRPKQACCRCDHIVQAPVPSKPIARSYAGAGLLAHVVTGKYADHLPLYRQSEIYRRQ
                     GVELSRATLGRWTGAVAELLEPLYDVLRQYVLMPGKVHADDIPVPVQEPGSGKTRTAR
                     LWVYVRDDRNAGSQMPPAVWFAYSPDRKGIHPQNYLAGYSGVLQADAYGGYRALYESG
                     RITEAACMAHARRKIHDVHARAPTYITTEALQRIGELYAIEAEVRGCSAEQRLAARKA
                     RAAPLMQSLYDWIQQQMKTLSRHSDTAKAFAYLLKQWDALNVYCSNGWVEIDNNIAEN
                     ALRGVAVGRKNWMFAGSDSGGEHAAVLYSLIGTCRLNNVEPEKWLRYVIEHIQDWPAN
                     RVRDLLPWKVDLSSQ"
     gene            complement(4970..5392)
                     /locus_tag="ACHURS_001757"
     CDS             complement(4970..5392)
                     /locus_tag="ACHURS_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFR1237513.1"
                     /translation="MADNRKCSFYIYPERNAADRVADRFLEKLPQKERGRAMRAMMLC
                     GAALMKQDERLPFLIAEFLTDSTSMQDIQRIISSTLPQQENGEVVRLLEAFLQSAGNN
                     AKTILPAVDSATQEISAPVDQNLLETRNNIKNLFPDDE"
     gene            complement(5395..6375)
                     /locus_tag="ACHURS_001758"
     CDS             complement(5395..6375)
                     /locus_tag="ACHURS_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFR1237514.1"
                     /translation="MRIFVDDGSTNIKLAWVEDGAVKTLISPNSFKPEWSMTLQGLTD
                     ATIPASANYEIDGEKYSFDQLSPDAVRTTETRYQYSDVNVVAIHHALMQSGIAPQPVD
                     IIVTLPLGEYLDENDQPNLANIERKKNNVRRAVTVQGRENFTIRKVSVLPESVPAGFD
                     VLKDLNDLESLLIVDIGGTTLDIAHVRSKMSGFTKTYCDPKTGVSIITEAVKHAMDVN
                     VSTRTSSYFADRLIQSRDDNEFLTRYIPNAEQREQILTVLRDREKKLQHRVADSVGRF
                     AGFTHVMVVGGGASLVAGAVKKATGVGDNRFFVSDNPQFDLVLGMMAMKG"
     gene            6589..6696
                     /locus_tag="ACHURS_001759"
     CDS             6589..6696
                     /locus_tag="ACHURS_001759"
                     /inference="COORDINATES: protein motif:HMM:NF025189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HFR1237515.1"
                     /translation="MGPEKWLRYVIEHIQDWPANRVRDLLPWKVDLSSQ"
     gene            complement(6764..7330)
                     /locus_tag="ACHURS_001760"
     CDS             complement(6764..7330)
                     /locus_tag="ACHURS_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1237516.1"
                     /translation="MIIYFFPFQMEENDAFLDAEVKYQKMKNKECYGVKASHKTPLSF
                     LQPSDTLYIVAHGNTSVIGSGSATGPTLNPVALATLLMNKRLPKNFIDIRVLSCASGI
                     HSRTPAFAQRLKEIMKVHGYHSLVVTGYLGEVDVSRDWRLKNDDGMEFYTLRKKGIIP
                     VKDVLSESQRALCGSDLKYALSDFKKRF"
     gene            7978..8535
                     /locus_tag="ACHURS_001761"
     CDS             7978..8535
                     /locus_tag="ACHURS_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFR1237517.1"
                     /translation="MSTHDSLIELTDTLIQQNGYQGFSYADLAKTLGIRKASIHYHFQ
                     TKTDLGIAYCEYKKAGFLALEATLQQLPAGKARLKGYMEAFLACADTGQMCGVYAMMS
                     DCNLFEPPLQEAVNRLAQTDLRILTAVLLSGKESGELFFSAPAEDVAMIVASSIKGAL
                     MLNRIPPHDACIRTMKALEQLLCRV"
     gene            8844..9134
                     /locus_tag="ACHURS_001762"
     CDS             8844..9134
                     /locus_tag="ACHURS_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HFR1237518.1"
                     /translation="MKKTTSTALLALTLGSVLAAASAPAMATGDNMEKCYGVSLKGKN
                     DCKAGAGTTCAGTAKMDYQGNAWKLVPAGTCVKMKSPGSPTGYGQLEAFKNA"
     gene            9166..10092
                     /locus_tag="ACHURS_001763"
     CDS             9166..10092
                     /locus_tag="ACHURS_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family multinuclear iron-containing
                     protein"
                     /protein_id="HFR1237519.1"
                     /translation="MMTSQTHPCDPSASTASGMWNACLPARGGISLKPEHFRQLLADK
                     PDTGFLEIHAENYLCAGGPFHQALTALCECYPLSIHGVGMSIGGEQPLNTAHLQRVAA
                     LVDRYQPHSFSEHLAWSTHDDIFLNDLLPLPYTQATLNRVCEHIDQVQNTLRRQILLE
                     NPATYVEFSTSTFSETDFIREVIHRTGCGLLLDVNNLYISSVNHNRDPYQMLHQLPLD
                     RVREIHLAGYTESQDGVGARLLIDSHDSPVTVPVWQLYESALTGTGAVATLIEWDASI
                     PPLSVLLGEAAMAENRMKEGARHAKQSVPGTA"
     gene            10061..10819
                     /locus_tag="ACHURS_001764"
     CDS             10061..10819
                     /locus_tag="ACHURS_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001579652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding domain-containing protein"
                     /protein_id="HFR1237520.1"
                     /translation="MQNSLYQALLEPHSPPPVGLTVWNGSDPAVRFAVYRNNIMASLT
                     EALAENCPVLQRQVGDEFFRAMVAEFIRQHPPSSPVLAGYGHALPAWIASFSPLAEWP
                     WLADLTRLELLFIDALHAAETTSLVSEEQLVRLAQTGLVSLTAGLVPSVRLFSSPYAI
                     YSLWAAHQEEEPLFVDPLQPEQVLLFRLGDDVRITLLSPADFSFMVAVHAGNNLAQAT
                     LQAREHDCCFEPGSLLLRLHQNRLLGWLACTEEK"
     gene            10821..11348
                     /locus_tag="ACHURS_001765"
     CDS             10821..11348
                     /locus_tag="ACHURS_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001669399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFR1237521.1"
                     /translation="MALMRSFLQRGQALLQDSLIPLLARTGLAATFWLSGQSKVEGLV
                     LDILGGMPPELDVPHITRSAVALFATEYRLPFLAPETAALLAAGAEHIFPVLLVLGLA
                     TRFSALVLAGMTIVIQVFVYPDAWPVHATWLTAQMYLLAYGGGKYSLDNLLKRTYRRR
                     HQNVMQKRNNPMMTL"
     gene            complement(11681..12043)
                     /locus_tag="ACHURS_001766"
     CDS             complement(11681..12043)
                     /locus_tag="ACHURS_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HFR1237522.1"
                     /translation="MTNFKSDLNLIRKEWYNAFYSGNTEMLDYLEADWFISTNGQKVI
                     YKKHQLRKIDMRPARILPGIERYEYDVVMREFKGIACVSGKAEIKQNDGNILMAFIEN
                     WIKVNGGWKMQFISYEKI"
     gene            complement(12428..12529)
                     /gene="fmt"
                     /locus_tag="ACHURS_001767"
                     /pseudo
     CDS             complement(12428..12529)
                     /gene="fmt"
                     /locus_tag="ACHURS_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="modifies the free amino group of the aminoacyl
                     moiety of methionyl-tRNA(fMet) which is important in
                     translation initiation; inactivation of this gene in
                     Escherichia coli severely impairs growth; incomplete;
                     partial in the middle of a contig; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
     gene            complement(12544..13053)
                     /gene="def"
                     /locus_tag="ACHURS_001768"
     CDS             complement(12544..13053)
                     /gene="def"
                     /locus_tag="ACHURS_001768"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFR1237523.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIRRIVDDMFETMYAEKG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            13581..13928
                     /locus_tag="ACHURS_001769"
     CDS             13581..13928
                     /locus_tag="ACHURS_001769"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1237524.1"
                     /translation="MARSAKPRKRKPAPQRSKLPRYVVKLHPDDFFDEEDAEVLHFDN
                     FDDAVECCADLGIPFFLDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILAGIRGLT
                     LSGTKTHVKGFWS"
     gene            complement(14023..14883)
                     /locus_tag="ACHURS_001770"
     CDS             complement(14023..14883)
                     /locus_tag="ACHURS_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="HFR1237525.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            complement(15066..15623)
                     /locus_tag="ACHURS_001771"
     CDS             complement(15066..15623)
                     /locus_tag="ACHURS_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005687610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR1237526.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTD
                     REGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGD
                     MGQMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTG
                     ATEIAHQLSIARSTVYKILEDERAS"
     gene            15787..18792
                     /locus_tag="ACHURS_001772"
     CDS             15787..18792
                     /locus_tag="ACHURS_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /protein_id="HFR1237527.1"
                     /translation="MPVDFLTTEQTESYGRFTGEPDELQLARYFHLDEADKEFIGKSR
                     GDHNRLGIALQIGCVRFLGTFLTDMNHIPSGVRHFTARQLGIRDITVLAEYGQRENTR
                     REHAALIRQHYQYREFAWPWTFRLTRLLYTRSWISNERPGLLFDLATGWLMQHRIILP
                     GATTLTRLISEVREKATLRLWNKLALIPSAEQRSQLEMLLGPTDCSRLSLLESLKKGP
                     VTISGPAFNEAIERWKTLNDFGLHAENLSTLPAVRLKNLARYAGMTSVFNIARMSPQK
                     RMAVLVAFVLAWETLALDDALDVLDAMLAVIIRDARKIGQKKRLRSLKDLDKSALALA
                     SACSYLLKEETPDESIRAEVFSYIPRQKLAEIITLVREIARPSDDNFHEEMVEQYGRV
                     RRFLPHLLNTVKFSSAPAGVTTLNACDYLSREFSSRRQFFDDAPTEIISRSWKRLVIN
                     KEKHITRRGYTLCFLSKLQDSLRRRDVYVTGSNRWGDPRARLLQGADWQANRIKVYRS
                     LGHPTDPQEAIKSLGHQLDSRYRQVAARLCENEAVELDVSGPKPRLTISPLASLDEPD
                     SLKRLSKMISDLLPPVDLTELLLEINAHTGFADEFFHASEASARVDDLPVSISAVLMA
                     EACNIGLEPLIRSNVPALTRHRLNWTKANYLRAETITSANARLVDFQATLPLAQIWGG
                     GEVASADGMRFVTPVRTINAGPNRKYFGNNRGITWYNFVSDQYSGFHGIVIPGTLRDS
                     IFVLEGLLEQETGLNPTEIMTDTAGASELVFGLFWLLGYQFSPRLADAGASVFWRMDH
                     DADYGVLNDIARGQSDPRKIVLQWDEMIRTAGSLKLGKVQVSVLVRSLLKSERPSGLT
                     QAIIEVGRINKTLYLLNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYT
                     DGQEDQLGTLGLVTNAVVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINML
                     GHYSFTLAELVTKGHLRPLKEASEAENVA"
     gene            18901..20088
                     /locus_tag="ACHURS_001773"
                     /pseudo
     CDS             18901..20088
                     /locus_tag="ACHURS_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element IS91 family transposase"
     gene            complement(20448..20930)
                     /locus_tag="ACHURS_001774"
     CDS             complement(20448..20930)
                     /locus_tag="ACHURS_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1237528.1"
                     /translation="MRNKTREAMRLFLGGRCYTAEKLEKDYLAEVANYSNDRWEAPQR
                     AARLAASVKRYKTSEMLRFIFATIAYDPDPDLTPLTVRRLCKALFGRTGSQWLVVEVF
                     GEKGRQHRSADSNPEMVEKMAARYRHAAELHWSATLAEIERVKRLYQTKIKKSKKEVG
                     "
     gene            complement(21047..21895)
                     /locus_tag="ACHURS_001775"
     CDS             complement(21047..21895)
                     /locus_tag="ACHURS_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFR1237529.1"
                     /translation="MNNDELVTRRAQAIAEDRCFSKGRLRDEFRMKPAPGAEPVKWYK
                     NSYGGRFAVYRIADCVPMCEKRPLTSKQQLAGQRLSVLSRLNSTSGRMARQAYDWLSL
                     APLFLDTETTGLDNTAEALEIGLTDAAGQVVFETRLKPTVAIGVQAAAVHGISEQALC
                     GAPSWTDVARQLRHAIGDRPVIIFNSRFDIRILKQTAAAHSDPADWLEEMTVYCAMEL
                     AAGYYGATNRYGTISLASAASQAGLTWEGQAHSAIADARMAAGVVNAIAAYHLELLQE
                     QARLKI"
     gene            complement(21941..22219)
                     /gene="yacB"
                     /locus_tag="ACHURS_001776"
     CDS             complement(21941..22219)
                     /gene="yacB"
                     /locus_tag="ACHURS_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin YacB"
                     /protein_id="HFR1237530.1"
                     /translation="MEIFWTMLASQDRKRIREYIAEQNLIAAIELDERIGYSASSLAG
                     LPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVYILRVLHTAQKWP"
     gene            complement(22219..22488)
                     /gene="yacA"
                     /locus_tag="ACHURS_001777"
     CDS             complement(22219..22488)
                     /gene="yacA"
                     /locus_tag="ACHURS_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015056448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin YacA"
                     /protein_id="HFR1237531.1"
                     /translation="MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNTWFRDQVAAGRQQLERGDVLTHDMVESSAAAWRDEMSRKVAGK"
     gene            complement(23400..24257)
                     /gene="repA"
                     /locus_tag="ACHURS_001778"
     CDS             complement(23400..24257)
                     /gene="repA"
                     /locus_tag="ACHURS_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFR1237532.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPRKGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAAAAARRSRVVWENKQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDAGRERQDIVTLVKRQLTREIAEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            complement(24250..24324)
                     /gene="tap"
                     /locus_tag="ACHURS_001779"
     CDS             complement(24250..24324)
                     /gene="tap"
                     /locus_tag="ACHURS_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFR1237533.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            complement(24569..24817)
                     /locus_tag="ACHURS_001780"
     CDS             complement(24569..24817)
                     /locus_tag="ACHURS_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFR1237534.1"
                     /translation="MSQTENAVTSSSGTKRAYRKGNPLTLAERQQASLARKRATHKEL
                     RVFIPAALKAQLQVMCEAEGVTQAEMIAELIKQKSAFS"
BASE COUNT         6170 a         6478 c         6271 g         6135 t
ORIGIN      
        1 atataccggc gtccggactg aagagcggcc acgcaggttg atccgctgcc tgcaaacggg
       61 tccagcacaa tcgcgttcgg atgtgtgaag ctctcaatga gcggttgcag gctggtaacg
      121 ggcttttcag tcgggtgatg acgattgcct gaatatttcc agcccagcac gtcgggcaat
      181 ggcttctgag gcagcgccgg acgtcctttt gccaggatgt aggcgcattc gtggcgatag
      241 cccacatatg cggccttcga tgtgtagttt ttggtgaaga ccaggtgacc aacaacgccg
      301 aatcccgcat ttttccaggc ggccataaag cgatcgacgc ggttccagcc gtagaagctc
      361 accatcagcg cgtctttttt gagtacgcgg tacatttcat tacaggccgg ttgcagccat
      421 tcatcggttt tatcgccagc gatggtgcgc ccgaaacggt cacgaaaacc gacgaggtac
      481 ggcgggtcag tcagtatgaa atcaatggcg ttatcaggaa tacgggccat aacatcgatg
      541 cagttaccga ggacaaaacg ggacataaca acgctccttc tcttacagag gacaccggga
      601 gggcacccct gtggtgcccg ttccccgctg cctgtgggct gttcaccgcg tccggtaacg
      661 ggtcaggccg accgtggaat accgcagccg tcgtctttct gacggcaagg ctatgccgcg
      721 aaagcggctt gaagggcgtt catcagaaaa gggcgacagg ctggcgtaca ctgtcaccga
      781 cgccagccgg gagcggtaaa agcgcccgtc ccgtgcccgc cggacgcggg acactgacca
      841 caggcagcgt ttatgccgcg ccgcgtcagc cggcgcagta aaaagaggag cacggcgaca
      901 tcctttgtcg gaaaagaaac gggcggttgc ccgcccgtct ccgtcatact tcatccgcca
      961 taatgcgtca cattgtcgac tatccacgca ggtccggtga cttccggcag ctcatcgtca
     1021 tctgtcgggg aagaccattc cagttccccc cacagcacat caacaagctc tccgcgctca
     1081 tacaactgcc agccacagaa attgcagccc tgttcggcgt accagtgctc cagcgtgcag
     1141 ccgaaacggc ggcttaattc tgcaacaacg tcgctttccg gctgacacca cggcgtgtcg
     1201 aaatccacct gaatgaagtt ttccccctga ctgctgatat tcaggtcata cccacaaggc
     1261 cacttcacgc cataccgttc tgtataccag tgatatgcag aaggaacacc gttcagaaca
     1321 ccgccattga agccgttcac ttcaacatcc aggcgggtgg gcagaaccat ccgcaggtca
     1381 aacggcatgg ttttttccgg cagtacgttg tcacacagac ggttccacca caccgataca
     1441 tcctcgttac tccagatgtc gcaccagtca cctctttttg gggtgaaaag tgcggtgatg
     1501 ctttccctga catcatccgg taacccttcc cagcgacgct gtccagtacc gctctgcagc
     1561 cagagttcat gcagcatcag gcagttttgc tcatccagca gtacaccgtt ctgcaggtgt
     1621 gtcagccagc gggtgaacgc gatattttcc ggcgatacaa caccacgtcc ggcagccgtc
     1681 agtccggggc acggttcaaa ctgcacatct tcagtggtct gcagaagtcc ggcacttccg
     1741 gccagaaaca gctgaatacc ttcatttgtg gcgcggcgat aaaacggtgt gactgcaccg
     1801 ctggccagtc gtttaatctc agcaatctgt gccggttcac cagaaaaatg catacgattt
     1861 gagcaccagt ttggcatggg aattctcctc tcaggagcac cgggagggag cccctgtggc
     1921 gcccgttccc cgctgcctgt gggctgttca ccgcgtccgg taacgggtca ggccgaccgt
     1981 ggaataccgc agccgtcgtc tgtttgacgg caaggttatg ccgcgaaagc ggcttgaagg
     2041 gcgttcatca gaaaagggcg acgggctggc gtacactgtc accgacgcca gccgggagcg
     2101 gtaaaagcgc ccgtcccgtg cccggccgga cgcgggacac tgaccacagg cagcgttcct
     2161 gccgcgccgc gtcagccggc gcagtaaaaa gaggagcccg gcaacatcct ttgtcggaaa
     2221 agaaaaaagg acgcaacagc gtcctttaac gtaaccggct catttaaacc gtctggtctg
     2281 tttcctccgg ctctacaaaa ataatgtcca tcatttttaa tggacactat cgtatgaaac
     2341 accggacctg gatcactgaa gctttacgtc ttcactttga agaacattta ccccgggttg
     2401 tggccgggcg tcgcctgggt gtaccaaaat caacagtttg tagtatgttc gtgcgctttc
     2461 ggagagctgg cctttcgtgg cctttgcccg caggcatgtc ggagcaggaa cttgatgcct
     2521 gcctttacgg acaattttcc acggtaccag tcgtacgtcc tgaaagcacc gttatatccg
     2581 aagcccccgt ggtaaaaaaa cgtccccggc ggcccaactt cccttatgag tttaaaatcg
     2641 ccttagtgga gcagtcactg cagcccggag cctgtgtggc gcagatcgcc cgggaaaacg
     2701 gaatcaacga taacctgctc ttcaactggc gccatcaata ccggaaaggt ggcctgctgc
     2761 cttccggaaa aaatatgccg gcactgcttc ccgtgacgtt aacgccggag ccggataata
     2821 aaatcccggc ccccgcacag gaaccagagc agataaatac accgtccgac agtctgtgtt
     2881 gtgagctggt tctgccggcc ggaactctca ggcttaaagg taaactgacg ccggcgttat
     2941 tacagacact tatccgcgaa ataaaaggga gcagccactg atgatatctc tccctgcagg
     3001 ttcgcgtatc tggctggttg caggtatcac cgatatgcga aatggcttta acggcctggc
     3061 atcaaaagtt cagaacgtcc tgaaggatga cccgttctcc ggacacctgt tcatcttccg
     3121 cggacgccgg ggtgaccaga taaaagtgtt gtgggctgac agtgacggac tgtgcctctt
     3181 caccaaacgc ctggagcggg gccgcttcgt ctggccagtc acccgtgacg gcaaggtgca
     3241 ccttactccg gctcagttat ccatgcttct tgaaggtatc aactggaagc acccgaaacg
     3301 aacggaacgc gctggaatcc gcatataacc cgttgtaaag tgaggatatg gacacctcac
     3361 ttgctcatga gaacgcccgc ctgcgggcac tgttgcagac gcaacaggac accatccgcc
     3421 agatggctga atacaaccgc ctgctctcac agcgggtggc ggcttatgct tccgaaatca
     3481 accggctgaa ggcgctggtt gcgaaactgc aacgtatgca gttcggtaaa agctcagaaa
     3541 aacttcgtgc aaaaaccgaa cggcagatac aggaagcaca ggagcgaatc agcgcacttc
     3601 aggaagaaat ggcggaaacg ctgggtgagc aatatgaccc ggtactgcca tccgccctgc
     3661 gccagtcttc agcccgtaaa ccgttaccgg cctcacttcc ccgtgaaacc cgggttatcc
     3721 ggccggaaga ggaatgctgt cctgcctgtg gtggtgaact cagttctctg ggatgtgatg
     3781 tgtcagagca actggagctt atcagcagcg cctttaaggt tatcgaaaca caacgtccga
     3841 aacaggcctg ttgccggtgc gaccatatcg tgcaggcacc agtaccttca aaacccattg
     3901 cacgcagtta tgccggagcg gggcttctgg cccatgttgt caccgggaaa tatgcagacc
     3961 atctgccgtt ataccgccag tcagaaatat accgtcgtca gggagtggag ctgagccgtg
     4021 ccacactggg gcgctggaca ggtgctgttg ctgaactgct ggagccgctg tatgacgtcc
     4081 tgcgccagta tgtgctgatg cccggtaaag tccatgctga tgatatcccc gtcccggtcc
     4141 aggagccggg cagcggtaaa acccggacag cccggctgtg ggtctacgtc cgtgatgacc
     4201 gtaacgccgg ttcacagatg cccccggcgg tctggttcgc gtacagtccg gaccggaaag
     4261 gtatccatcc acaaaattac ctggccggtt acagcggtgt gcttcaggcc gatgcttacg
     4321 gtggttaccg ggcgttatac gaatccggca gaataacgga agccgcgtgt atggctcatg
     4381 cccggagaaa aatccacgat gtgcatgcaa gagcgcccac ctacatcacc acggaagccc
     4441 tgcagcgtat cggtgaactg tatgccatcg aggcagaggt ccggggctgt tcagcagaac
     4501 agcgtctggc ggcaagaaaa gccagagccg cgccactgat gcagtcactg tatgactgga
     4561 tacagcaaca gatgaaaaca ctgtcgcgtc actcagatac ggcaaaagcg ttcgcatacc
     4621 tgctgaaaca gtgggatgca ctgaacgtgt actgcagtaa tggctgggtg gaaatcgaca
     4681 acaacatcgc agagaacgcc ttacggggag tggccgtagg ccggaaaaac tggatgttcg
     4741 cgggttccga cagcggtggt gaacatgcgg cggtgttgta ctcgctgatc ggcacatgcc
     4801 gtctgaacaa tgtggagcca gaaaagtggc tgcgttacgt cattgaacat atccaggact
     4861 ggccggcaaa ccgggtacgc gatctgttgc cctggaaagt tgatctgagc tctcagtaaa
     4921 tatcaatacg gttctgacga gtcgcttacc ctttaacaga gttcagttct tattcatcat
     4981 ccggaaacaa gttcttaatg ttgtttcttg tttccaggag attctgatcg actggagcag
     5041 atatttcttg cgtagcagaa tcaaccgcag gcaggatcgt ttttgcatta ttaccggcag
     5101 actgcagaaa tgcctccagc aaacgaacaa cttcaccatt ttcctgttgt ggtagcgtac
     5161 tactgataat tcgttgaata tcctgcatag acgtgctgtc tgtcaggaat tcagctatca
     5221 ggaacggcaa tcgttcatcc tgtttcatca atgcagcacc acacaacatc atggcccgca
     5281 ttgcccgacc acgttctttc tgaggcaatt tttcaagaaa cctgtcagct acccgatcag
     5341 ctgcatttcg ttccggataa atataaaaag agcacttacg attatcagcc atactcatcc
     5401 cttcattgcc atcattccca acaccagatc gaactgagga ttgtcactga caaagaaacg
     5461 gttatcaccc actcctgtcg ctttttttac tgccccagcc acaaggctgg caccaccgcc
     5521 aacaaccata acatgagtaa aaccagcaaa gcgtcctact gaatccgcca cacgatgctg
     5581 cagttttttt tcccggtcac gtaatactgt caggatctgc tcacgctgtt ctgcattcgg
     5641 gatataacga gtcaggaatt cattgtcatc ccgactctgg attagacgat ctgcaaaata
     5701 agaactggtt cgtgtactca cattcacatc catcgcatgc ttaactgctt cagtaatgat
     5761 ggaaacgcct gtcttggggt cacaataagt ttttgtgaaa ccggacattt tgctgcggac
     5821 atgtgcaata tcaagggtcg ttccaccgat atcaacgatc aacagcgact ccagatcatt
     5881 cagatctttc agcacgtcaa aaccggcagg cacggactca ggcaagacac tgactttacg
     5941 aatggtaaag ttttcacgcc cctgaactgt tacagctcgt ctgacattat ttttcttacg
     6001 ttcgatatta gccagatttg gctgatcgtt ctcatccaga tactcaccta aaggtaaagt
     6061 aacaataata tcaacaggtt gcggagcaat acctgactgc atcaacgcat gatgaatagc
     6121 aaccacatta acgtcgctgt actgataacg cgtttctgtg gtgcgcactg catccggact
     6181 caattgatca aagctgtatt tctctccatc aatctcatag tttgcggaag cgggtatagt
     6241 ggcatcagtc agtccctgca gcgtcataga ccattccggt ttaaaactat tcggactaat
     6301 taacgttttc actgcaccgt cttcaaccca ggccagtttg atgttggtag aaccgtcatc
     6361 tacaaagata cgcatccaga acctcacaga ataaaatgga tttttttaga aaaatatggt
     6421 gccgaaaaag attatcacct aaaaacacgc atcagtaaag acctgatatc gcccacaaac
     6481 agaaaaataa agaaaaatga agataaaaat cgactaaaac agcacacagg aaacaaacaa
     6541 gaacaataaa ggacaaatac agttaaaatt cagaaaaaac tgaacaatgt ggggccagaa
     6601 aaatggctac gttatgtcat tgagcatatc caggactggc cggcaaaccg ggtacgcgat
     6661 ctgttgccct ggaaagttga tctgagctct cagtaaatat caatacggtt ctggcgagcc
     6721 gcttacttgc ataacggctc tgacgtcagt gcactactcc tattcaaaat cgtttcttga
     6781 aatcagaaag ggcgtatttc agatcagaac cacaaagcgc tctctgggat tcacttaata
     6841 catccttaac aggaataatc cctttttttc ttaaggtata aaactccata ccatcatcat
     6901 tttttaatct ccagtctctc gatacatcaa cctcaccaag gtatccggta acgaccagac
     6961 tatgataccc atgtactttc ataatttctt tcagccgttg tgcgaaggct ggggttctcg
     7021 aatgtatccc cgaggcgcag gacaacactc tgatatctat gaagttcttt ggtaaacgtt
     7081 tgttcattaa tagagtggct aatgctacgg gattcaacgt tggcccagtt gctgaacctg
     7141 acccaataac tgatgtgttg ccatgagcaa cgatgtataa cgtatcgctc ggttgaagaa
     7201 aggataaagg tgttttgtgt gaagctttca caccataaca ttctttattc ttcattttct
     7261 gatacttaac ttcggcatca agaaaagcgt cattttcttc catttgaaat gggaaaaagt
     7321 aaataatcat attacctctc gacatcagaa aaaattaaaa tactccacat gcggaaagct
     7381 ctgagcagga aacatcacca aggacgaaac ctcctctctc tgcaaaggat agttatccat
     7441 ccggggcaac ctataccttt accataactt ccggcatagc caccttgcta caatcaaggg
     7501 gtgttgaaga aacatccgtt tcgtggtgct tttttagtct tttggggatt taaattccta
     7561 tcgatgaacg gggaggagat ctggccctgg atgttaccaa ccgatccccg cagacatttg
     7621 ctttcagaac agtctgaaat atcagtaata tctgcaacag gatgccattc cgggctcagt
     7681 aaggcacttc ttcccctgac ttacgtgtct tcagcaattt gagcacccat gtatactgct
     7741 gcaaaatggc atctgaatcg atcacggttt ggccaatccg tgatcgaata gagcggaata
     7801 cgcaccggtg cggacatcgt gtaatccggg catgtgacgg aagtgctgaa acagacccag
     7861 gagcagcagt catcttcgct ttgcagtaaa ataccagtac agatttcctg cctggcggcg
     7921 tttgtcttcg ggcctgttgt tattcagaat tactatagcc ccccccggga gaaacatatg
     7981 tccacccatg acagtctgat tgaactgact gacacactta tccagcagaa cggttaccag
     8041 gggttcagct atgctgacct ggcgaagacg ctgggtatcc gtaaagccag cattcactac
     8101 catttccaga caaaaacaga cctgggaata gcctactgtg aatataaaaa agccggtttt
     8161 ctggcgctgg aagccaccct gcagcagtta cctgccggga aagcccgcct gaagggctat
     8221 atggaggcct ttctggcctg cgcggacacc ggccagatgt gcggggtgta cgcaatgatg
     8281 tcggactgta acctgtttga gcctcccctg caggaggccg ttaaccggtt ggcacagacg
     8341 gacctgcgca ttctgacggc ggtcctgctg tccggaaagg aaagcggaga actgtttttt
     8401 tctgccccgg cagaagatgt tgctatgatt gtggccagtt cgataaaagg cgccctgatg
     8461 ctgaaccgca taccgcctca cgacgcctgc atccgcacga tgaaagcgct ggagcagttg
     8521 ctctgccggg tctgactcat ccctgcggac gtgtcccggt gatgccgggg cgaccccctg
     8581 tcattttgag cggtccgact ggacgttacc ggagtgatcc ggaacctctc aaggacaggc
     8641 aactcagcat cagccatggt ctgccaggca taactgcctg tatttatttt ccagaccggg
     8701 tgcaacctgc ctcccgtatt gtgaagaaaa tctcgttttc cggacataaa gaaaaaaaac
     8761 acattgagaa cttatctatt agtagatagt tttaaaggca tcagcaatgt gctgataaat
     8821 acaacgactc agaggatatg aatatgaaaa aaacaacctc caccgcatta ctggcactga
     8881 ccctgggcag cgtactggca gcggccagtg ccccggcaat ggccactggc gataatatgg
     8941 aaaaatgtta cggcgtttcg ctgaaaggca aaaacgactg taaagccggc gccggcacaa
     9001 cctgtgcagg caccgcgaaa atggattatc agggaaatgc ctggaaactg gtaccggcgg
     9061 gcacctgcgt gaagatgaaa tccccgggtt cccctaccgg ctatggccag cttgaggcct
     9121 ttaaaaacgc ataatatcca cgacagtcca caaccaggag ccgatatgat gaccagccag
     9181 actcacccct gtgatccctc tgccagcact gcttctggta tgtggaatgc gtgcctcccc
     9241 gcccggggag gcatcagcct gaaaccggag catttccggc aacttctggc agataaaccc
     9301 gatacgggct ttctggaaat tcatgcagag aattatctct gtgccggggg gcctttccac
     9361 caggcgctga cggccctgtg tgaatgctat cccctgtcca ttcacggggt ggggatgtcc
     9421 atcggtggag aacaaccgct gaataccgca catctgcagc gggtggcggc cctggttgac
     9481 cgctatcagc cgcattcatt ttcagagcat ctggcctggt ccacgcatga tgatattttt
     9541 ctgaatgatc tcctgccgtt accgtacaca caggccacgc tgaaccgggt atgtgaacat
     9601 atcgatcagg tccagaatac gctgcgtcgc cagatattgc tcgagaatcc ggccacctat
     9661 gttgaattct ctacctccac cttcagtgag accgatttta tccgtgaggt gatccaccgt
     9721 accggctgcg gactgttgct cgatgtgaat aacctctata tcagcagcgt gaatcacaat
     9781 cgcgatccgt accagatgtt gcatcaactg cccctggacc gggtgaggga aatccatctt
     9841 gcaggctata ccgaaagcca ggacggcgtc ggtgcccgcc tgcttattga cagtcatgat
     9901 agcccggtga cggtgccagt ctggcagctt tatgagtccg ccctcaccgg cacaggtgcg
     9961 gtcgccaccc tgatcgaatg ggatgccagt atccccccgc tcagcgtcct gctcggtgag
    10021 gcagcaatgg cagaaaacag aatgaaagaa ggagccaggc atgcaaaaca gtctgtacca
    10081 ggcactgctt gagccacact cacccccccc ggtggggctt accgtctgga atggcagcga
    10141 tccggccgtc cggtttgcgg tctaccgtaa taatatcatg gcctccttaa cggaagcgct
    10201 ggcagaaaac tgcccggtcc tgcaacgaca ggttggtgac gaattttttc gggccatggt
    10261 tgctgaattt atccgtcagc atccaccctc gtcgccggta ttggcggggt atggacatgc
    10321 tctgcctgcc tggatagcgt ccttttcccc actggctgaa tggccgtggc tggccgatct
    10381 gacccgactg gaactattgt ttatcgatgc gttgcatgcg gcggagacga ccagcctcgt
    10441 atccgaagaa cagctggtgc gactggcgca gaccggactg gtcagtctga ctgcgggcct
    10501 ggttccttct gtccgacttt tcagctcgcc ttatgcgata tacagcctgt gggcagctca
    10561 tcaggaggag gagccattgt ttgttgaccc tctgcagcca gagcaggtgc tgttgttccg
    10621 tttgggggat gacgtgcgga ttacgcttct cagccctgcc gacttttcgt ttatggtggc
    10681 ggtacatgcc ggcaataacc tggcgcaggc aacgttgcag gccagagagc atgactgctg
    10741 ttttgaacct ggctctctgt tgctgcgcct gcaccagaac aggctactcg ggtggctggc
    10801 ctgcacagag gagaaatgat atggcattga tgagaagttt tctgcagcgg ggccaggcat
    10861 tactgcagga cagtctgatc ccccttctgg ctcgtacagg cctggccgcc acattctggc
    10921 tgtcagggca aagtaaggta gaggggttgg ttctggatat actgggaggg atgccaccgg
    10981 aactggatgt tccgcatatt acccgttcgg cggttgcgct gtttgccaca gagtaccgac
    11041 tgccgtttct ggcaccggag actgctgcac tgcttgcagc aggagccgaa catatttttc
    11101 cggttctgct tgtcctggga ctggcaacgc gtttttccgc actagtgctg gcaggaatga
    11161 cgatagtgat ccaggtgttt gtctatcccg atgcctggcc ggttcatgcc acctggctta
    11221 ccgcccagat gtatttactg gcgtatggcg gcggaaaata ttccctggat aacctgctga
    11281 aacgtacgta ccggcgtagg caccagaatg tgatgcagaa aaggaataac cctatgatga
    11341 cgctataatc ttaaataatg tccgggatgg catataagct acttcttcag gaaaaggact
    11401 caattgggag ggcatgaaaa accctggggc aatagctatg ccgccatgaa ctatttaaaa
    11461 catcatttga tgaattaaaa aacaatccat atattcggga acctatagag catggtgatt
    11521 tattatctga taaagagaaa ataaaaacta taataactaa tactataaat agttttgata
    11581 ttcagtttgc agaaaacatg cttaaagctc aaagcagaag ttatcgctat aaaaaataaa
    11641 atttgaacat atataaccat taatctaagg gggcttcccc ttagattttt tcatacgata
    11701 taaactgcat tttccatcca ccattaacct ttatccaatt ttcaataaat gccattaata
    11761 tatttccatc attttgcttt atttcagctt tccctgaaac acaagcaatt cctttaaact
    11821 cacgcattac gacatcatat tcatatcttt ctatgccagg taaaatcctc gctggacgca
    11881 tatcaatctt tcgtaactga tgttttttat agataacttt ttgaccattt gttgaaataa
    11941 accaatcagc ttccaagtaa tctagcattt cagtattacc tgaataaaat gcattatacc
    12001 attcttttct tattagattt aagtctgatt taaaattagt cacactacct tcctcatatc
    12061 taattcctcc tctaatctat ccccaacatt ccttactgat tttatagcat catcaatagc
    12121 cacattatat atggctggga ttactctttt aagaatctca tcgagtaggc agcctggcgg
    12181 ctgcggcttg tcatggcctg gaattaccgt tataaaaaat gataatgtca ttgtctttca
    12241 ggtagttata tggcccgttc agctaaatta ccggaaacgg agcaaaaaac cgccattcat
    12301 accctgtatc aatcagtgtc atcctgttta atagtcgttt ccgctcatat ggtgcacaag
    12361 gggtgttgaa gaaacatcct tttcgtggtg cttttttagt cttttgggga ttttaattcc
    12421 tatcgatgaa cacgccaacg acgttatgac cagaagacaa cagcgcgtcg agatgacgcg
    12481 ctgcaaagtc aggtgtaccc gcaaaaataa tacgtagtga ttctgacacg ttagttctta
    12541 tccttaagcc cgggctttca gacgatccag tttttcaact ttctgacgaa tacgttgttg
    12601 tttcagcggt gacagataat ccataaacag tttgccgacc aggtgatcca tctcatgctg
    12661 aatacagatg gctaacagac cctctgcttc cagttcaaat ggtttaccgt cgcggtccag
    12721 ggcgcgaatt ttaactttct ctgcgcgtgg cactaaagct cgttgttcag ggatcgacag
    12781 gcatccttct tcaatgcctg tttcgccgct tttttctaaa agctccggat tgattaacac
    12841 cagccgttcg tcacggtttt ccgaaacatc aataacaatg atacgttgat ggatatcgac
    12901 ctgggttgcc gccaggccaa tacctttttc tgcgtacatc gtctcgaaca tatcatcgac
    12961 gatacgccga atttctgcat tcacttcttc taccggttta gcaactttgc gaagccgctc
    13021 gtccggaata tgtaacactt gcaaaactga cataagtctc cagagatgtg ttcaggagtt
    13081 agaaagatta tttcttctat tctagacaaa tccccctctg attgacagca tcactgacca
    13141 atcgcaaaga tttctaaggc tgcttatggc agggagataa ggatggggtt ctacgccgga
    13201 accccagatt tttccgttat cccagtttta aaccggctac taattgccgg cctgacacag
    13261 gctaagtgtg cccgggaatc ggtgcaagta taaaaaacaa cctgtcacgg tataaaaatc
    13321 gaccatgcag agagagaaac caccggagtg gtgggaaaag cagaaaggct cacgtgtcat
    13381 aaggcgtgga cagcggtatc gtctggtttt caatttccga gcctgtacat agatttgtgt
    13441 aattgcctga ttttgatatg ttcaatccag catcaaatga aggttaattt atcgagtagg
    13501 cagcctggcg gctgcggctt gtcatggcct ggaattaccg ttataaaaaa agataatgtc
    13561 attgtctttc aggtagttat atggcccgtt cagctaaacc ccgtaaacgc aaacccgcac
    13621 cacaaagaag caaacttccc cgctatgttg tgaaacttca tccggatgat ttttttgacg
    13681 aagaagacgc tgaagttctg cactttgata attttgacga tgccgttgag tgctgcgctg
    13741 acctgggtat tccgttcttt ctggatgcag gaaacaaaaa gctggtcttc tggtttgttc
    13801 gtgtcgatga cgaagggtat ccggaaatag cccgatgtac ggagcgggag tttgcaacca
    13861 ttcttgccgg tatcaggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
    13921 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
    13981 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
    14041 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
    14101 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
    14161 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
    14221 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
    14281 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctgc
    14341 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
    14401 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
    14461 tccgatcgtt gtcagaagta agttggcagc agtgttatca ctcatggtta tggcagcact
    14521 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
    14581 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac
    14641 acgggataat accgcaccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
    14701 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
    14761 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
    14821 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacgaaaat gttgaatact
    14881 catactcttc ctttttcaat attattgaag catttaccag ggttattgtc tcatgagcgg
    14941 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    15001 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
    15061 gcgtatcacg aggccctttc gtcttcaaga attttataaa ccgtggagcg ggcaatactg
    15121 agctgatgag caatttccgt tgcaccagtg cccttctgat gaagcgtcag cacgacgttc
    15181 ctgtccacgg tacgcctgcg gccaaatttg attcctttca gctttgcttc ctgtcggccc
    15241 tcattcgtgc gttctaggat cctccggcgt tcagcctgtg ccacagccga caggatggtg
    15301 accaccattt gccccatatc accgtcggta ctgatcccgt catcaatgaa ccggactgcc
    15361 acgccctgag cgtcaaattc ctttatcagt tggatcatat cggcagtgtc gcggccaaga
    15421 cggtcgagct tcttaaccag aatgacatca ccttcctcca ccttcatcct cagcaaatcc
    15481 agcccttccc ggtctgttga actgccggat gccttatcgg taaatatacg gtttgctttc
    15541 acacctgcgt ctttgagtgc tctgacctga agatcaagag actgctgact ggttgagacc
    15601 cgagcgtaac caaaaagtcg cataaaaatg taccttaaat cgaatatcgg acaactcatg
    15661 tctattatta caaatttacg atttaataga catattaatg taacagtttt acgatgtccg
    15721 ataatttata acatttcgta cggttggaaa aatgttacta aatgcccgtc aggcagggag
    15781 gccgatatgc ccgttgactt tctgaccact gagcagactg aaagctatgg cagattcacc
    15841 ggtgaaccgg atgagcttca gctggcacga tattttcacc ttgatgaagc agacaaggaa
    15901 tttatcggaa aaagcagagg tgatcacaac cgtctgggca ttgccctgca aattggatgt
    15961 gtccgttttc tgggcacctt cctcaccgat atgaatcata ttccttccgg cgtccggcat
    16021 tttaccgcca gacagctcgg gattcgtgat atcaccgttc ttgcagaata cggtcagagg
    16081 gaaaataccc gccgtgagca tgcagcgctg atacgtcagc actatcagta tcgtgaattt
    16141 gcctggccct ggacatttcg ccttacccgt cttttatata cccggagctg gataagcaac
    16201 gaacgtcctg gcctgctttt cgatctggcg acagggtggc ttatgcaaca tcgtattatt
    16261 ctccccggag ccactacgct gacccggttg atttcagagg taagggaaaa ggcgacgttg
    16321 cgcctgtgga acaaactggc actgataccg tcagccgaac agcgttcaca gctggagatg
    16381 ctgctggggc caactgattg cagccgcctg tctttactgg aatcactgaa aaagggccct
    16441 gtgaccatca gtggtccggc gtttaatgaa gcaattgaac gctggaaaac tctgaacgat
    16501 tttggcctgc atgctgaaaa cctgagtaca ctcccggctg tgcgcctgaa aaatctcgca
    16561 cgttatgctg gtatgacttc ggtgttcaat attgccagga tgtcaccgca gaaaaggatg
    16621 gcggttctgg ttgcctttgt ccttgcatgg gaaacgctgg cgctggatga tgcattggac
    16681 gttctggacg ccatgctggc cgttatcatc cgtgacgcca gaaagattgg gcagaaaaaa
    16741 cggctccgct cgctgaagga tctggataaa tctgcattgg cgctcgccag cgcatgttcg
    16801 tacctgctga aagaagaaac accggacgaa tcgattcgtg ctgaggtgtt cagctacatc
    16861 ccaaggcaaa agctggctga aatcatcacg cttgtccgtg aaattgcccg gccctcagac
    16921 gataattttc atgaagaaat ggtggagcag tacgggcgcg ttcgtcgttt cctgccccat
    16981 ctgctgaata ccgttaaatt ttcatccgca cctgccgggg ttaccactct gaatgcctgt
    17041 gactacctca gccgggagtt cagctcacgg cggcagtttt ttgacgacgc accaacggaa
    17101 attatcagtc ggtcatggaa acggctggtg attaacaagg aaaaacatat cacccgcagg
    17161 ggatacacgc tctgctttct cagtaaactg caggatagtc tgaggcggag ggatgtctac
    17221 gttaccggca gtaaccggtg gggagatcct cgtgcaagat tactacaggg tgctgactgg
    17281 caggcaaacc ggattaaggt ttatcgttct ttggggcacc cgacagaccc gcaggaagca
    17341 ataaaatctc tgggtcatca gcttgatagt cgttacagac aggttgctgc acgtctttgc
    17401 gaaaatgagg ctgtcgaact cgatgtttct ggcccgaagc cccggttgac aatttctccc
    17461 ctcgccagtc ttgatgagcc ggacagtctg aaacgactga gcaaaatgat cagtgatcta
    17521 ctccctccgg tggatttaac ggagttgctg ctcgaaatta acgcccatac cggatttgct
    17581 gatgagtttt tccatgctag tgaagccagt gccagagttg atgatctgcc cgtcagcatc
    17641 agcgccgtgc tgatggctga agcctgcaat atcggtctgg aaccactgat cagatcaaat
    17701 gttcctgcac tgacccgaca ccggctgaac tggacaaaag cgaactatct gcgggctgaa
    17761 actatcacca gcgctaatgc cagactggtt gattttcagg caacgctgcc actggcacag
    17821 atatggggtg gaggagaagt ggcatctgca gatggaatgc gctttgttac gccagtcaga
    17881 acaatcaatg ccggaccgaa ccgcaaatac tttggtaata acagagggat cacctggtac
    17941 aactttgtgt ccgatcagta ttccggcttt catggcatcg ttataccggg gacgctgagg
    18001 gactctatct ttgtgctgga aggtcttctg gaacaggaga ccgggctgaa tccaaccgaa
    18061 attatgaccg atacagcagg tgccagcgaa cttgtctttg gccttttctg gctgctggga
    18121 taccagtttt ctccacgcct ggctgatgcc ggtgcttcgg ttttctggcg aatggaccat
    18181 gatgccgact atggcgtgct gaatgatatt gccagagggc aatcagatcc ccgaaaaata
    18241 gtccttcagt gggacgaaat gatccggacc gctggctccc tgaagctggg caaagtacag
    18301 gtttcagtgc tggtccgttc attgctgaaa agtgaacgtc cttccggact gactcaggca
    18361 atcattgaag tggggcgcat caacaaaacg ctgtatctgc ttaattatat tgatgatgaa
    18421 gattaccgcc ggcgcattct gacccagctt aatcggggag aaagtcgcca tgccgttgcc
    18481 agagccatct gtcacggtca aaaaggtgag ataagaaaac gatataccga cggtcaggaa
    18541 gatcaactgg gcacactggg gctggtcact aacgccgtcg tgttatggaa cactatttat
    18601 atgcaggcag ccctggatca tctccgggcg cagggtgaaa cactgaatga tgaagatatc
    18661 gcacgcctct ccccgctttg ccacggacat atcaatatgc tcggccatta ttccttcacg
    18721 ctggcagaac tggtgaccaa aggacatctg agaccattaa aagaggcgtc agaggcagaa
    18781 aacgttgctt aacgtgagtt ttcgttccac tgagcgtcag acccctatca gtgccggtgg
    18841 tatgtactgc ccggaatgcg gcacagttca ctggccggat ggcgttaccc cacccgtctg
    18901 atgcttcccc gttttgccga tatttttcag cagggtaacc gctggcttaa ctggctggag
    18961 aaacagccgg aaggttcagt gcgtccggtg gtgactgagt cagtgacaaa aatcatggca
    19021 tgcgggacca cgctgatggg gtatacacag tggtgctgtt catcaccgga ctgttgccac
    19081 acaaaaaagg tctgcttccg gtgtaaaagt cgctcctgcc cgcactgcgg ggtgaaggct
    19141 ggcgcacagt ggatatagta tctgctgagc ctggtccccg actgcccgtg gcagcatatt
    19201 gtgttcacac ttccctgcca gtactggtcc ctggtgttcc acaacaggtg gttactggca
    19261 gagatgagcc gcattgctgc ggatgtgata ctggaaatct gccatcaggc agatgtggag
    19321 ccggggatat tcacggtgat ccacacatgg gggcgtgacc agcagtggca tccgcatatc
    19381 catttatcga caactgccgg tggtgtgacg tcgggccaca cctggaaaaa tcttcatttt
    19441 tacgcccgta aggtgatgag catgtggcgt taccggataa cgcggctact gtcccggaaa
    19501 tacccggagc tggtaatacc ggatgaactg gcagtggaag gaaacagcaa acgggactgg
    19561 aatcgcttcc tggacacgca ttaccgccgc ggctggaatg tcaacatatc cagggtgatg
    19621 gataacgcca cacatgtggc ggtgtacttc ggctcttacc tgaaaaagcc accggtgccg
    19681 atgagtcggc tggagcatta tgccggtcag gatgaaatcg gtctgcgtta caacagtcac
    19741 cgtacaaaac gggaagaata cctgttgatg agtggagatg agttcatgga aaggttctcc
    19801 tggcatgtag cagataaggg gttccgtatg gtgaggtact acggtttcct gagtccggtg
    19861 aagcgccgct tactggaaga agttgtgtac gtcataacgg agacggtgag aaaaacggcg
    19921 atgcaaatca ggtggagagg gatgtatcag aggttactga aggttgaccc gctgaagtgc
    19981 attctgtgcg gaagtcagat gcgttttacg gggctgaagc ggggttaccg actggcagag
    20041 ctggtcctga tgcatgagcg actggcacga cagcaggtgt gcggctgaga gccgcagagg
    20101 ggaagttgcg tccattttac cggaaacgga gcaaaaaacc gccattcata ccctgtatca
    20161 atcagtgtca tcctgtttaa tagtcgtttc cgctcatatg gtgcacaagg ggtgttgaag
    20221 aaacatccgt tttgtggtgc ttttttagtc ttttggggat ttaaattcct atcgatgaac
    20281 aggaaatatt atcttaatcc gcgtaagtgg gccgtgaggg ccgcttacaa gcgtgtatga
    20341 gatcagtttg gatattcgaa attattgtac gtttaacgca ccatacatta atgctaatgt
    20401 atgcatacat tagcattaat gatgaaggaa aagaataaca atggcagtca acccacttct
    20461 tttttggatt tcttaatttt cgtctgataa agccgcttta cccgttcaat ttctgccagc
    20521 gttgcggacc agtgtagttc ggcggcatga cgatatctgg cagccatttt ttccaccatc
    20581 tccggattgc tgtccgcgct gcggtgctga cgccccttct ccccaaaaac ttcaacaacc
    20641 agccactggc ttccggttct gccgaacaat gccttgcaca aacgtctcac agtcagcggg
    20701 gttaaatctg gatcgggatc ataggcaatg gtagcaaaaa taaagcgcag catttctgat
    20761 gtcttgtaac gctttacgct ggcagccagg cgtgcggcac gttgcggtgc ttcccagcgg
    20821 tcgttactgt aattagccac ttcagccaga taatcctttt caagtttttc agccgtgtag
    20881 cacctgccgc ctaaaaaaag ccgcatggcc tccctggttt tatttctcac atatcatccc
    20941 tcaccaatgc taatgtacgc atacattagc attaacatac tgacagtaac aagcggcgga
    21001 tttacgtctg gataacgtaa atgattgcgg tatacaggcc agtcggtcag attttcagcc
    21061 gggcctgttc ctgcagcagt tccagatgat atgcagcaat agcgtttacc acccctgccg
    21121 ccatccgtgc gtcggcgatc gctgagtgtg cctgcccttc ccaggtcagt ccggcctggc
    21181 tggcagcact ggccagtgaa atcgtgccat agcggttggt ggccccataa tatcctgcag
    21241 ccagctccat cgcacaatat accgtcattt cttccagcca gtcagccgga tcgctatgtg
    21301 cggccgcagt ctgtttcaga atgcggatgt cgaaccggga attaaagata attactggtc
    21361 gatctccgat tgcatgccgc agttgccgcg ccacatctgt ccatgatggt gcgccgcaga
    21421 gagcctgctc actgatacca tgcacggcgg cggcctgcac cccgatggcg accgtgggct
    21481 tcagcctggt ttcaaatacc acctgaccgg ccgcatcggt caggccgatt tccagggctt
    21541 ccgcggtatt atccagtcct gtcgtttcag tatccagaaa gagtggtgcc agactcagcc
    21601 agtcatatgc ctgccgtgcc atccggccac tggtgctgtt tagtcgggat aaaacggaaa
    21661 gccgctggcc agccagctgt tgtttactcg tcagagggcg tttctcacac atcggaacac
    21721 agtctgcaat tcggtataca gcgaacctgc caccataact atttttatac cacttaaccg
    21781 gctcagcacc gggggctggc ttcattctga attcatcgcg cagacgtcct ttcgagaaac
    21841 acctgtcttc ggcaatcgcc tgcgcacgtc tggtgaccag ctcatcatta ttcatggttc
    21901 ttgcatctcc gtcagacgtt tttatatccg gacagcccct ctatggccac ttctgtgcag
    21961 tatgcaatac tcgcaggata tacacttttc cccactggct gtcgacctca taaaccagaa
    22021 caaaatgcgg gtgaataacc agttcccggg taccttccac acgaccgttg cggcctttat
    22081 acggcagccc ggccaggctg ctggctgaat aaccaatccg ttcgtccagt tcaatggcgg
    22141 ctatcaggtt ctgctccgca atgtactcac gaatgcgctt cctgtcctgg ctggccagca
    22201 tggtccagaa tatctccatc atttaccggc gaccttcctg ctcatttcat cccgccatgc
    22261 agcggcagaa gattcgacca tgtcatgggt aagcacatcg ccgcgctcga gttgctgacg
    22321 tcctgccgca acctggtcac ggaaccaggt attatgctgc cgttcaacgg tctggcgcat
    22381 aaaatcccgg attaactgag agccattacg gtccatgctt tttgcagcgg ccataaaagc
    22441 atccttcagt tcagcgtcga ttcttaaact catattaacc tgtgccattt atcacctcgt
    22501 cattactacg tatatacaca gtatataacg attcagatat cgtcacaagg atatgccgcg
    22561 ccagcggcat ggaaggcggc actctgcggt tacatgtgat accggagtaa aaccgccgaa
    22621 gcccggcgta agccggtacc gattgacaga tttcacctta cccatcccca tcctgctaag
    22681 ccatacccgc tttcagccat gagagagctt ttgtgcgcgg tcggagtggt cccgacaagg
    22741 gtttacccgc agtcggggcg tatctccgcg ttagcgggcc gtcagggccg cttacgagcg
    22801 tgtactgagg acttccagcc agaagactga cagcgatgat aatgtagtta caacattcat
    22861 aattaaaagc gactctgttc cggcccgaag ggccggggcg gggccgcttt tcagttatga
    22921 ggaggggctt tgtggtttcg gttctgcgct ggtccggggt ttttctggag gttgtttttg
    22981 tgtgttgtaa ctaaagtggc tccggttggg gcccgccgct tgcggtggga ggtgcatatc
    23041 tgtctgtcca caggacaggc agtgaatagg ttttcttttt aaatgaatgt aattaagtag
    23101 tttaaaggag atataaacag gtgtttaaaa gatacattgc accctgtaag actggcggct
    23161 ggcgctttat gacacgaacg gttgtaacct tatagtaaag tcccttgcag ttaaatgtgg
    23221 ataagcaaaa ttacctgtct gtgaggcgtg ttttgtatca aaaacagggg ggaccggatg
    23281 caccagaagg tggatgatga gattgttttt tgtatgtggt gctgattttt tgtgcgctgt
    23341 cgggcttcag acgtgcggaa gcctccggcg caggccggat tattctgagg aggtcacttt
    23401 cagggggaag cggtggccag ccggctgtaa ttgcggttac gtgacagaat catgcgctcc
    23461 ttcacacgac gctccacttc acgttttacc gcctcacgac tggcagtgaa gcgcccttcc
    23521 gcgatttcac gcgtcagctg ccgtttcacc agggtgacga tatcctgacg ttccctgccc
    23581 gcatcacgac gcgcacgggc acgctttatt ccccgggact taagctctgt ctgataactg
    23641 cgaaaacgct cacgaacaaa acgccaggct ttcgctatca gttcatccat gcccagggta
    23701 tccagcccct gctttttgcg ttgtttgttt tcccatacca cacggctgcg gcgcgcggcg
    23761 gccgctgcct cctctgatac atcgagggca gcaaacagtg cagatgtgaa cgtgatatcg
    23821 gtcggaatgt agcacccgat aagcgggtca tattccgtct ggtaggtaat cagtcccagc
    23881 tctgacagga acgtcagggc ccgggtggca cgggtgatgg agagttttcc ggcagcagac
    23941 tccgtcgcca gtccgcactc aatggccagc gtggtgatgg agcactggac gcggttggcc
    24001 agcgggtcat agtggaaaca cagcccctgc aggagcgcat caatagcccg tcgacgcagc
    24061 accggtggca tgcgtcgacg cagaccacgc gaacgggcat gcgccacatg aatggcgaaa
    24121 tcaaaacggg aagtgaagcc caccgccttt tccatcagtt tttcgcagaa cttcagcgtt
    24181 ccggcacctt tacggggtgt aaacaccgga ttcgggttct ttacctggcg gtaatacgtt
    24241 tgttgaagat cagtcacacc atcctgcact tacaatgcgc agaaggagtg agcacagaaa
    24301 gaagtcttga acttttccgg gcatataact atactccccg catagctgaa ttgttggcta
    24361 tacggttttt agatggcccc ggtaatcttc tcagtcgcca aacttgtcga agattatcgg
    24421 ggttttcact tttctggctc ctgtaaatcc acatcagaac cagttccctg ccaccttacg
    24481 gcgtggccag ccacaaaatt ccttaaacga tcagcaatct atcacgcaca ccagaaacag
    24541 gcaagaatgt gatgtcgcta cattttagtt agctaaaagc actcttctgc tttatcagtt
    24601 cggcaatcat ctcagcctgg gtaacgccct cagcttcaca catcacctgc aattgcgctt
    24661 ttaacgcagc aggaatgaac actctgagtt ctttatgtgt agctcgtttt cttgccaggg
    24721 atgcctgctg acgttctgca agcgtcagag gattaccttt tctgtatgct cgctttgtac
    24781 ctgatgagga agtcactgca ttttctgtct gcgacatctc gcctcctcaa tactcaaaca
    24841 gggatcgttt cgcagaggat actacagttt tttgaaatca gcgacttgag aattgtgacg
    24901 aagatctgtg ttggcgcaca aatcaacggg gattactgtt gtttaatgtg atttttgttg
    24961 cgaaatggct gaaaaaacag gaaatgcctt aacccgggag ccggccccgg gcgggaccgg
    25021 ctgtttctgg catgtcgggt gtctgcccct gaaa
//
LOCUS       DAZPDU010000048        24934 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZPDU010000048 DAZPDU010000000
VERSION     DAZPDU010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24934
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.048"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..163)
                     /locus_tag="ACHURS_001781"
     CDS             complement(<1..163)
                     /locus_tag="ACHURS_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFR1237535.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFA"
     gene            complement(382..1359)
                     /gene="epmA"
                     /locus_tag="ACHURS_001782"
     CDS             complement(382..1359)
                     /gene="epmA"
                     /locus_tag="ACHURS_001782"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFR1237536.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1684..3492
                     /gene="frdA"
                     /locus_tag="ACHURS_001783"
     CDS             1684..3492
                     /gene="frdA"
                     /locus_tag="ACHURS_001783"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFR1237537.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3485..4219
                     /gene="frdB"
                     /locus_tag="ACHURS_001784"
     CDS             3485..4219
                     /gene="frdB"
                     /locus_tag="ACHURS_001784"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFR1237538.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4230..4625
                     /gene="frdC"
                     /locus_tag="ACHURS_001785"
     CDS             4230..4625
                     /gene="frdC"
                     /locus_tag="ACHURS_001785"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFR1237539.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4636..4995
                     /gene="frdD"
                     /locus_tag="ACHURS_001786"
     CDS             4636..4995
                     /gene="frdD"
                     /locus_tag="ACHURS_001786"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFR1237540.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5058..6191
                     /locus_tag="ACHURS_001787"
     CDS             5058..6191
                     /locus_tag="ACHURS_001787"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFR1237541.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6280..6813
                     /gene="blc"
                     /locus_tag="ACHURS_001788"
     CDS             6280..6813
                     /gene="blc"
                     /locus_tag="ACHURS_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFR1237542.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6810..7127)
                     /gene="sugE"
                     /locus_tag="ACHURS_001789"
     CDS             complement(6810..7127)
                     /gene="sugE"
                     /locus_tag="ACHURS_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFR1237543.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7302..7448)
                     /gene="ecnB"
                     /locus_tag="ACHURS_001790"
     CDS             complement(7302..7448)
                     /gene="ecnB"
                     /locus_tag="ACHURS_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFR1237544.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7559..7684)
                     /gene="ecnA"
                     /locus_tag="ACHURS_001791"
     CDS             complement(7559..7684)
                     /gene="ecnA"
                     /locus_tag="ACHURS_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFR1237545.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(7736..8302)
                     /gene="efp"
                     /locus_tag="ACHURS_001792"
     CDS             complement(7736..8302)
                     /gene="efp"
                     /locus_tag="ACHURS_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFR1237546.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8344..9372
                     /gene="epmB"
                     /locus_tag="ACHURS_001793"
     CDS             8344..9372
                     /gene="epmB"
                     /locus_tag="ACHURS_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFR1237547.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPGELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPILRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            9762..10631
                     /locus_tag="ACHURS_001794"
     CDS             9762..10631
                     /locus_tag="ACHURS_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFR1237548.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLNAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGTVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(10834..11187)
                     /locus_tag="ACHURS_001795"
     CDS             complement(10834..11187)
                     /locus_tag="ACHURS_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFR1237549.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(11325..12971)
                     /gene="groL"
                     /locus_tag="ACHURS_001796"
     CDS             complement(11325..12971)
                     /gene="groL"
                     /locus_tag="ACHURS_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFR1237550.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(13015..13308)
                     /locus_tag="ACHURS_001797"
     CDS             complement(13015..13308)
                     /locus_tag="ACHURS_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFR1237551.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            13584..14840
                     /gene="yjeH"
                     /locus_tag="ACHURS_001798"
     CDS             13584..14840
                     /gene="yjeH"
                     /locus_tag="ACHURS_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFR1237552.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAAQVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LTCFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(14862..15332)
                     /locus_tag="ACHURS_001799"
     CDS             complement(14862..15332)
                     /locus_tag="ACHURS_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFR1237553.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDR"
     gene            15669..17105
                     /gene="aspA"
                     /locus_tag="ACHURS_001800"
     CDS             15669..17105
                     /gene="aspA"
                     /locus_tag="ACHURS_001800"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFR1237554.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            17223..18524
                     /gene="dcuA"
                     /locus_tag="ACHURS_001801"
     CDS             17223..18524
                     /gene="dcuA"
                     /locus_tag="ACHURS_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFR1237555.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            18640..18978
                     /gene="cutA"
                     /locus_tag="ACHURS_001802"
     CDS             18640..18978
                     /gene="cutA"
                     /locus_tag="ACHURS_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFR1237556.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            18954..20651
                     /gene="dsbD"
                     /locus_tag="ACHURS_001803"
     CDS             18954..20651
                     /gene="dsbD"
                     /locus_tag="ACHURS_001803"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFR1237557.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSHFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTHLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGMLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPIFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKKFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            20688..21263
                     /locus_tag="ACHURS_001804"
     CDS             20688..21263
                     /locus_tag="ACHURS_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1237558.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            21370..21445
                     /locus_tag="ACHURS_001805"
     tRNA            21370..21445
                     /locus_tag="ACHURS_001805"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21403..21405,aa:Phe,seq:gaa)
     gene            21688..21802
                     /locus_tag="ACHURS_001806"
                     /pseudo
     CDS             21688..21802
                     /locus_tag="ACHURS_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            22062..22361
                     /locus_tag="ACHURS_001807"
     CDS             22062..22361
                     /locus_tag="ACHURS_001807"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFR1237559.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYK"
     gene            complement(22352..22477)
                     /gene="laoB"
                     /locus_tag="ACHURS_001808"
     CDS             complement(22352..22477)
                     /gene="laoB"
                     /locus_tag="ACHURS_001808"
                     /inference="COORDINATES: protein motif:HMM:NF038068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arginine responsive protein LaoB"
                     /protein_id="HFR1237560.1"
                     /translation="MVRLWEGEFVSGTASGIGGGEDSIISSPSSSLYQITGTIHL"
     gene            22544..23599
                     /gene="cadC"
                     /locus_tag="ACHURS_001809"
     CDS             22544..23599
                     /gene="cadC"
                     /locus_tag="ACHURS_001809"
                     /inference="COORDINATES: protein motif:HMM:NF007540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFR1237561.1"
                     /translation="MVFFLLSLGICVALVAFSTLDTRLPMSKSRILLNPRDIDINMVN
                     KSCNSWSSPYQLSYAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVN
                     QRQYRAQQCFMSVKLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQR
                     MQETLQQILPHRGALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEK
                     ALVDIVRHSQHPLDEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKIDESY
                     QAINTGIDLEMSWLNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQ
                     TSVPYVVPYLDKFLASE"
     gene            23964..>24934
                     /gene="cadB"
                     /locus_tag="ACHURS_001810"
     CDS             23964..>24934
                     /gene="cadB"
                     /locus_tag="ACHURS_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFR1237562.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLYLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLL"
BASE COUNT         6222 a         6267 c         6122 g         6323 t
ORIGIN      
        1 aggcgaatgg aataaagaat aacaatgcag aaaatatata aaagggaatc gcactataac
       61 ccattaagta ataagccgat gggctattgg caaatccaaa tacggaagta aagatcatca
      121 atatgagtcc tatcagactc atctttttta tcgtgtgagg catgaaacca tccttcattt
      181 cagcgcgtgt ataacacgtg cccactgaac tgcactgcgc aacattgagc aggcagcgta
      241 aaaaccaata cagaattgat acccggatga tatcaaatat ccagtcttaa attgtggcct
      301 ttaacggaaa aatgaaaaca tatgctacag aatggcgctt atcacgccat tcttcgctgt
      361 taattcagta atttttcaga attatgcccg gtcaacgcta aaggcgatga cttcagccag
      421 tgtctccgcg cccagcgcca acatcaccag acgatcaaca cctaatgcca cgccggaaca
      481 gtcaggcata ccgactttca aggcttcaat cagattctgg tcaatggggt gctgcggcaa
      541 accgcgcgcc gcgcgcttac ggttatcttg ttcaaagcgt tgttgctgct cacgggcatc
      601 cgttaattca tggaaaccat tcgccagctc aataccttta taataaacct caaagcgttc
      661 agcgacccga tgatcttcgg tactgatttg cgccagtgat gcctggctgg ctggaaagtg
      721 gtacacaaag gtcggttttt ctttgccaat atttggctct acgccaaagg taaacagcaa
      781 ttgtagcaac gtgtcgcggt cttcttcggt atcagcaacg ttactcaaat ccagtttcgc
      841 tgcgacttcc cgcagttgcg ttttgtcggc agagagcggg tcaatttcca gataacgcaa
      901 gaaagcttgt tgataagaaa ggctttctgc tgccgggcag tccagcacct gttgtaagag
      961 atcgtccacc tcgttcatca accggtacat atcatagtgc ggtcgatacc actcaagcat
     1021 agtgaactca gggttgtgat aacgccccat ctcttcatta cggaagctgc ggcacagctg
     1081 gaataccggc ccacaaccgg caaccagcag gcgtttcata tggtattccg ggctggtcat
     1141 taaccagaga ttcatcccct gcgaatgccc ggggccaacg aaacgtgtct caaacgggac
     1201 caaatgaata tcggttaccg tcgcctggct catacaaggc gtctccacct ccagcactcc
     1261 acgatcggca aagaaacgac ggatctccgc cataatcgcc gcacgtttta ataagttagg
     1321 aatggatgcg ctcggctgcc aggatgccgt ttcgctcata gttaaatctc cagtttttga
     1381 caagggcacg aagtctactc gcaacgcgac ggcgagacaa attttacgca ggaatcaaac
     1441 agcggtgggc agtgactaaa aaaagcacga tctgatggtt tagtaattaa attaatcatc
     1501 ttcagtgata atttagccct cttgcgcact aaaaaaatcg atctcgtcaa atttcagact
     1561 tatccatcag actatactgt tgtacctata aaggagcagt ggaatagcgt tcgcagaccg
     1621 taactttcag gcacttaccc tgaagtacgg ggctgtggga taaaaacaat ctggaggaat
     1681 gtcgtgcaaa cctttcaagc cgatcttgcc attgtaggcg ccggtggcgc gggattacgt
     1741 gctgcaattg ctgccgcgca ggcaaatcca aatgcaaaaa tcgcactaat ctcaaaagta
     1801 tacccgatgc gtagccatac cgttgctgca gaagggggct ccgccgctgt cgcgcaggat
     1861 catgacagct tcgaatatca ctttcacgat acagtagcgg gtggcgactg gttgtgtgag
     1921 caggatgtcg tggattattt cgtccaccac tgcccaaccg aaatgaccca actggaactg
     1981 tgggggtgcc catggagccg tcgcccggat ggtagcgtca acgtacgtcg cttcggcggc
     2041 atgaaaatcg agcgcacctg gttcgccgcc gataagaccg gcttccatat gctgcacacg
     2101 ctgttccaga cctctctgca attcccgcag atccagcgtt ttgacgaaca tttcgtgctg
     2161 gatattctgg ttgatgatgg tcatgttcgc ggcctggtag caatgaacat gatggaaggc
     2221 acgctggtgc agatccgtgc taacgcggtc gttatggcta ctggcggtgc gggtcgcgtt
     2281 tatcgttaca acaccaacgg cggcatcgtt accggtgacg gtatgggtat ggcgctaagc
     2341 cacggcgttc cgctgcgtga catggaattc gttcagtatc acccaaccgg tctgccaggt
     2401 tccggtatcc tgatgaccga aggctgccgt ggtgaaggcg gtattctggt caacaaaaat
     2461 ggctaccgtt atctgcaaga ttacggcatg ggcccggaaa ctccgctggg cgagccgaaa
     2521 aacaaatata tggaactggg tccacgcgac aaagtttctc aggccttctg gcacgaatgg
     2581 cgtaaaggca acaccatctc cacgccgcgt ggcgatgtgg tttatctcga cttgcgtcac
     2641 ctcggcgaga aaaaactgca tgaacgtctg ccgttcatct gcgaactggc gaaagcgtac
     2701 gttggcgtcg atccggttaa agaaccgatt ccggtacgtc cgaccgcaca ctacaccatg
     2761 ggcggtatcg aaaccgatca gaactgtgaa acccgcatta aaggtctgtt cgccgtgggt
     2821 gaatgttcct ctgttggtct gcacggtgca aaccgtctgg gctccaactc cctggcggaa
     2881 ctggtggtct tcggccgtct ggccggtgaa caagcgacag agcgtgcagc aactgccggt
     2941 aatggcaacg aagcggcaat tgaagcgcag gcagctggcg ttgaacaacg tctgaaagat
     3001 ctggttaacc aggatggcgg cgaaaactgg gcgaagatcc gcgacgaaat gggcctggca
     3061 atggaagaag gttgcggtat ctaccgtacg ccggaactga tgcagaaaac catcgacaag
     3121 ctggcagagc tgcaggaacg cttcaagcgc gtgcgcatca ccgacacctc cagcgtgttc
     3181 aacaccgacc tgctctacac cattgaactg ggccacggtc tgaacgttgc tgaatgtatg
     3241 gcgcactccg caatggcacg taaagagtcc cgcggcgcac accagcgtct ggacgaaggt
     3301 tgcaccgagc gtgacgacgt caacttcctc aaacacaccc tcgccttccg cgatgctgat
     3361 ggcacgactc gcctagagta cagcgacgtg aagattacta cgctgccgcc agctaaacgc
     3421 gtttacggtg gcgaagcgga tgcagccgat aaggcggaag cagccaataa gaaggagaag
     3481 gcgaatggct gagatgaaaa acctgaaaat tgaggtggtg cgctataacc cggaagtcga
     3541 taccgcaccg catagcgcat tctatgaagt gccttatgac gcaactacct cattactgga
     3601 tgcgctgggc tacatcaaag acaacctggc accggacctg agttaccgct ggtcctgccg
     3661 tatggcgatt tgtggttcct gcggcatgat ggttaacaac gtgccaaaac tggcatgtaa
     3721 aaccttcctg cgtgattaca ccgacggtat gaaggttgaa gcgttagcta acttcccgat
     3781 tgaacgcgat ctggtggtcg atatgaccca cttcatcgaa agtctggaag cgatcaaacc
     3841 gtacatcatc ggcaactccc gcaccgcgga tcagggtact aacatccaga ccccggcgca
     3901 gatggcaaag tatcaccagt tctccggttg catcaactgt ggtctgtgct acgccgcgtg
     3961 cccgcagttt ggcctgaacc cagagttcat cggtccggct gccattacgc tggcgcatcg
     4021 ttataacgaa gatagccgcg accacggtaa gaaggagcgt atggcgcagt tgaacagcca
     4081 gaacggcgta tggagctgta ctttcgtggg ctattgctcc gaagtctgcc cgaaacacgt
     4141 cgatccggct gcggccattc agcagggcaa agtagaaagt tcgaaagact ttcttatcgc
     4201 gaccctgaaa ccacgctaag gagtgcaaca tgacgactaa acgtaaaccg tatgtacggc
     4261 caatgacgtc cacctggtgg aaaaaattgc cgttttatcg cttttacatg ctgcgcgaag
     4321 gcacggcggt tccggctgtg tggttcagca ttgaactgat tttcgggctg tttgccctga
     4381 aaaatggccc ggaagcctgg gcgggattcg tcgacttttt acaaaacccg gttatcgtga
     4441 tcattaacct gatcactctg gcggcagctc tgctgcacac caaaacctgg tttgaactgg
     4501 caccgaaagc ggccaatatc attgtaaaag acgaaaaaat gggaccagag ccaattatca
     4561 aaagtctctg ggcggtaact gtggttgcca ccatcgtaat cctgtttgtt gccctgtact
     4621 ggtaaggagc ctgagatgat taatccaaat ccaaagcgtt ctgacgaacc ggtattctgg
     4681 ggcctcttcg gggccggtgg tatgtggagc gcaattattg cgccggtgat gatcctgctg
     4741 gtgggtattc tgctgccact ggggctgttt ccgggtgatg cgctgagcta cgagcgcgtt
     4801 ctggcgttcg cgcagagctt cattggtcgc gtattcctgt tcctgatgat cgttctgccg
     4861 ctgtggtgtg gtttacaccg tatgcaccac gcgatgcacg atctgaaaat tcacgtgcct
     4921 gcgggcaaat gggttttcta cggtctggct gctatcctga cagttgtcac gctgattggt
     4981 atcgttacaa tctaacgtat cgccaatgta aatccggccc gcctatggcg ggccgttttg
     5041 tatggaaacc agaccttatg ttcaaaacga cgctctgcgc cttattaatt accgcctctt
     5101 gctccacatt tgctgcccct caacaaatca acgatattgt gcatcgcaca attaccccgc
     5161 ttatagagca acaaaagatc cccggtatgg cggtggcggt aatttatcag ggtaaacctt
     5221 attactttac ctggggctat gcggacatcg ccaaaaagca gcccgtcaca cagcaaacgt
     5281 tgtttgagtt aggttcggtc agcaaaacat ttacgggcgt gcttggtggc gacgctattg
     5341 ctcgagggga aatcaagtta agcgatcccg caacaaaata ctggcctgaa cttactgcta
     5401 aacagtggaa tgggatcaca ctattacatc ttgcgaccta caccgctggc ggcctgccat
     5461 tgcaggtgcc ggatgaagtg aaatcctcaa gcgacttgct gcgcttctat caaaactggc
     5521 agcctgcatg ggctccagga acacaacgtc tgtatgccaa ctccagtatc ggtttgttcg
     5581 gcgcactggc tgtgaagccg tctggtttga gttttgagca ggcgatgcaa actcgtgtct
     5641 tccagccact caaactcaac catacgtgga ttaatgtacc tcccccagaa gaaaagaatt
     5701 acgcctgggg atatcgcgaa ggtaaggcag tgcatgtttc gccaggggcg ttagatgctg
     5761 aagcttatgg tgtgaagtcg accattgaag atatggcccg ctgggtgcaa agcaatttaa
     5821 ccccccttga tatcaatgag aaaacgcttc aacaagggat acaactggca caatctcgct
     5881 actggcaaac cggcgatatg tatcagggcc tgggctggga aatgctggac tggccggtca
     5941 atcctgacag catcattaac ggcagtgaca ataaaattgc actggcagca cgcccggtaa
     6001 aagcgattaa gcccccaact cctgcagtac gcgcatcatg ggtacataaa acgggggcga
     6061 ccggcggatt tggtagctat gtcgcgttta ttccagaaaa agagctgggt atcgtgatgc
     6121 tggctaacaa aaactatccc aatccagcga gagtcgccgc cgcctgtcag attctcaacg
     6181 ctctacagta aaatttcatc gggtccgaat tttcggacct tttctgcgct tttccttgct
     6241 gtcatctaca cttagaaaaa aaccagtaag gaaacattta tgcgcctgct ccctctcgtt
     6301 gccgcagcga cagctgcatt tctggtcgtt gcctgcagtt ctcctacgcc gccgcgtggc
     6361 gtgacagtag tgaataattt cgacgctaaa cgctatctcg gtacctggta tgagattgcc
     6421 cgttttgatc accgttttga acgtggactg gaaaaagtca ccgcaacata cagtctgcgt
     6481 gatgacggcg gcctgaatgt catcaataaa ggctataacc ctgacagaga aatgtggcag
     6541 cagagtgaag ggaaagcgta ctttaccggc gcaccaacac gcgctgcgct gaaagtgtca
     6601 ttctttggtc ctttctatgg cggttataac gtcattgcac ttgatcggga ataccgccat
     6661 gcgctggttt gcggcccgga ccgcgactac ctgtggatac tctcccgcac gccaaccatt
     6721 tctgacgaag tgaaacagga gatgctggca gtcgcgaccc gggaagggtt tgatgtcagt
     6781 aagtttattt gggtacagca gcccggtagt tagtgagtgc tgagtttcag accaataatc
     6841 cccaatacga ttaacgccag actcgccagg cgcatcgggt tagcggactc accgagcagc
     6901 acaatgccgg tgatggccgc gccgactgcg ccaatacccg tccacacggc ataagccgtc
     6961 cctaccggta acgatttcat cgcccaggca agtagcgcca tactgactat catcgccgtc
     7021 acagtaataa cacttggcgt caaacgacta aagccgtggg tatatttcag gccaacggcc
     7081 cataccactt ccagcagacc agcaataact aagataatcc aggacatatc aggctccgga
     7141 gaattggggc cgtccccggt gaaaagaggc gtttgcaggt cgtcctgcaa agctgatggt
     7201 tgaacggata ttttgccagg tggcagggga atttcaacta ttacctttgt gactatcgca
     7261 aaaaaacaaa cggcacgaca taactgtcgt accgttattg cttattgctg cgctttcgtt
     7321 gctgcaccag aaatcgcgtt accgccatca gaaatgtctt caccaacgcc acgcgtggtg
     7381 ttgcaggcag ttaatactgt tgaaagcacc agaacagaaa agatcgctgc aattgtcttc
     7441 ttcaccataa cgtcttcctt ttaggtaatg ttatttatgt ttgcctatag caaattaata
     7501 atagacaaca tcgccaaaaa tgatggaaat ctgatgattt ttcggaagag aagaaaaatt
     7561 agctggcagc gcgggagatg gagttgccga gatgtttgat gtcttcgccg aaaccgcgag
     7621 cggtgttaca gcccgtgagc agcgtgctgg caagcaaaac aagaacgata aggcgtttca
     7681 tcatctctga cctgaataag tgatggtgca gcctgcaggc cgcaccacaa ccgcattact
     7741 tcacgcgaga gacgtattca ccagagcggg tatccacttt gatgacttcg ccgatttgta
     7801 cgaacagcgg aactttaacc acagcgccag tagacagggt agccggtttg ccgccagtac
     7861 ctgcggtatc acctttcagg cccggatcgg tatcaacgat ttccagttca acgaagttcg
     7921 gcggagtaac ggagatcggc tgaccattcc acagagttac gatacactct gcctgatcca
     7981 gcagccattt agcgttgtcg ccaattgctt ttgcatcagc agacagctgc tcgaaagttt
     8041 cgttgttcat gaagtgccag aactcaccgt cgttgtacag gtaagtcagg ttcatatcga
     8101 caacatcagc gccttcagcg gaatcagtag atttgaaggt tttttctacg cgagtaccgg
     8161 tcagcagacg acgcagttta acgcgagcaa atgcctggcc tttacccggt tttacgaatt
     8221 cactcgcttc aaccgcgtaa ggttcgccgt ctaacatgat tttaagacca gcacgaaaat
     8281 cgttgctata gtacgttgcc ataaggccct ctgaaatttg ttaattggta gctaagccac
     8341 aaaatggcgc atattgtaac cctaaatacc ccatccagag aagattggtt aacgcaactt
     8401 gccgatgttg tgaccgatcc tggtgaactt ctgcgtcttt tgaatataga cgcggacgaa
     8461 aaactgttgg ccggacgcag cgccaaaaag ctgtttgccc tgcgtgtgcc ccgctcattt
     8521 atcgatcgca tggagaaagg caatccggac gatcctattt tgcgtcaggt acttacctcg
     8581 caagatgagt ttgtcgtcgc gtccggattc tccaccgacc cactggaaga acagcacagc
     8641 gtagtgcctg gtttgttgca taaataccac aaccgggcgc ttttgctggt caaaggcggc
     8701 tgcgcggtaa attgccgcta ttgcttccgt cgccacttcc cctatgccga aaatcagggc
     8761 aacaagcgta actggcagac tgcacttgag tatgttgctg cgcatccgga actggacgag
     8821 atgattttct ccggcggcga tccgctgatg gcgaaagatc acgagctgga ctggttgctc
     8881 acacaactgg aagccatccc gcatatcaaa cgtctgcgga ttcacagccg tctgccgatt
     8941 gtgatcccgg cacgtatcac cgaggcgctg gttgaacgct ttgcccgttc tacgctgcaa
     9001 atcttgctgg tgaatcacat caatcatgcc aatgaggtag atgaaacgtt ccgtcaggcg
     9061 atggctaaat tgcgccgtgt cggtgtcacc ctgcttaacc agagcgttct gttacgtggt
     9121 gtgaacgata acgcacaaac gttggcaaac ctgagtaatg cgttgttcga tgccggcgtg
     9181 atgccgtatt acctgcatgt gctagataaa gtacagggcg cggcgcattt tatggtgagt
     9241 gatgacgaag cacggcagat tatgcgtgag ttgctgacat tggtgtcggg atatctggtg
     9301 ccgaaactgg cgcgagaaat tggcggcgaa cccagcaaaa cgccgctgga tctccagcta
     9361 cgccagcagt aactgattaa acagggaagt taaacattag cctgatgcgc tacgcttatc
     9421 aggcctacat tgtgcattga aattgaatgg atttgcaatg aactgtaggc cggataaggc
     9481 gtttacgccg catccggcaa gagcaacaaa cgcaagtaca gcaacctgtc attaaactgg
     9541 gaaatttaat ctttgcccgt tgcataacct ttaattattc atatacattg ctgttgttac
     9601 tctttatata gccaacacca ataattaacc tttcttttct gtttatatag agtgagtacg
     9661 gctatttatg ttattttaac aactattact accaagccca tttatctggc ttataaatta
     9721 aaattaatta tttaattgag aaatggttag ggagaaccta catggccata agcatcaagg
     9781 gtgttaatac cggtgttatt cgcaagagta ataatttcat tgcactggcg ctgaaaatta
     9841 aagaacctcg caataaagaa tcgttgttct ttatgtctgt catggaactg cgcgacctgc
     9901 tgattgcgct ggaaagtcgt ctgcatcaaa agcataaact gaatgctgct gcacgccttc
     9961 agtatgagca agctcgcgat aaagtcatta agaaaatggc agaaaacatc ccagaaattc
    10021 tggttgatga gcttaaaaat gccgatatta atcgtcgggt taacacgctg gaacttaccg
    10081 ataatcaagg cgaaaatctt accttcgtat taacgctaca tgacggtagc aaatgtgagt
    10141 tagtcgtcaa tgagttgcag attgaaatgc tggcgcgagc aatcattcat gcaattaata
    10201 atgcggaaat gcgtgaactg gcattacgta ttacctcact gttagatttc ctgccgttat
    10261 acgacgttga ctgccaggac aatggcaatc tggaatatga cacctattcc caaccggaat
    10321 ggaaacataa cctgttcgac cattatctgg cggtgcttta ccgctttaaa gatgaaagtg
    10381 gcaaagaaca atttagcggc acagtggtga aaacgcgtga agcaacgccg ggtaaagaaa
    10441 tagaggcgat cactcgccga atgcttgatt tcagcccacg actgaaaaaa ctcgcgggtg
    10501 tgccatgtca ggtctatgtt cgcaccgtag cggcgaataa cgcccaaccg ctaacccagg
    10561 atcaatgctt acgcgcgctg catcacttac gtgttcaatc caccagcaaa actgcaccac
    10621 aggcgaaata atctcatctt tgctaatgcg aggctggagc catccagcct gaaatgactg
    10681 ataacgtgat cgttggtcat gccggatgcg acgtaaacgc cttatccggc ctacaaattc
    10741 tcaagcattc aatcagttgc aatgcctgct gtaggcctga taagcgaagc gcatcaggca
    10801 ataatacgtt tgtcacccag cctctggatc acatcagttc gggcacttat aaacctgacc
    10861 gataatctgg ctatccgtcg ggacaaaact ggacaacatt ccctgcgacg ggctgctgat
    10921 gccataaatc acgttaccgc ccattgcagc cgcctggttg cgcagatcgt ttgctgcgcc
    10981 gcgcatagaa ccgccctctt ctccgtgttg cccggaaagc cagttgcttt gcttacctgt
    11041 cgcagtacca atcagctggc actctgcgcc tggctgctcg tccacaatgc gtacactctg
    11101 accggcagca ctcaattcgt tgctggagct acaacccgcc atcagtagcg cggcaccgac
    11161 aatccctgct aagtatttta cgtgcatgtt attccccata atcaatgagc tggacgccac
    11221 tcgcgtcgtc cgtgtctgaa tcttatacta gaaagatgac caaaagaaaa acccccagac
    11281 atttctgccc gggggtttgt ttatttctgc gaggtgcagg gcaattacat catgccgccc
    11341 atgccaccca tgccgcccat accgccagca gcgcctaagt cagctgcatc atttttcggc
    11401 aggtcggtaa ccatgcactc ggtggtgatc atcaggccag ccacagaagc cgcgtactgc
    11461 agagcagaac gggttacttt ggttgggtcc aggataccca tgtcgatcat gttgccgtat
    11521 tcttcggttg ctgcgttgta accgtagttg ccgtcgccgc ctttaacggt gttagcaaca
    11581 acagacggtt cttcgccgca gttcaggacg atctgacgca gcggagcttc cattgcacgc
    11641 agtgcaactt tgatacccac gttctggtct tcgttctgac cacgcaggtc agccagttta
    11701 gacgctacgc ggatcagcgc aacaccacca ccagcaacca cgccttcttc taccgcagca
    11761 cgggtcgcgt gcagggcgtc ttcaacgcgt gcttttttct ctttcatttc aacttcggta
    11821 gcagcaccta ctttgataac tgcaacgccg cctgccagtt tcgctacgcg ttcctgcagt
    11881 ttttcacggt cgtagtcaga agttgcttct tcaatctgct gacggatctg agcaacacgg
    11941 ccctggattg cagcttcttc acccacgcca tcgatgatag tggtggtgtc tttgttgatc
    12001 acaacacgtt tagcctgacc caggtcttcc agggttgctt tttccagttc cataccgatc
    12061 tcttcagaga tcacggtacc gccagtcagg gttgcgatat cctgcagcat agctttacga
    12121 cgatcgccga agcccggagc tttaactgca gcaactttca cgatgccacg catggtgtta
    12181 acaaccagag ttgccagcgc ttcgccttct acatcttcag cgatgatcag cagcggtttg
    12241 cctgctttgg caacggcttc cagaaccggc agcatttcgc ggatgttgga gattttcttg
    12301 tcagccagca ggatgaacgg gctttccagt tctactgcgc cagtttccgg cttgttgatg
    12361 aagtaaggag acaggtagcc acggtcgaac tgcatacctt caaccacgtc cagttcgtcc
    12421 tgcagaccgg taccgtcttc aacggtgata acgccttctt taccgacttt gtccatcgct
    12481 tcagcgatca gtttacctac ggtttcgtcg gagttagcgg agatggtacc aacctgagca
    12541 atcgctttag agtcagagca cggtacggac agcgctttca gttcttcaac tgcagcggta
    12601 acggctttgt cgataccacg tttcaggtcc atcgggttca tgcccgcagc aacagctttc
    12661 agaccctcag tgatgatagc ctgagccagt acagttgcag tggtggtacc gtcgcctgca
    12721 gcgtcgttcg ctttagaggc aacttctttc accatctgcg cacccatgtt ttcgaacttg
    12781 tcttccagtt cgatttcacg agcaacggaa acaccatctt tggtgatggt cggtgcaccg
    12841 aaagatttat ccagaactac gttacggcct ttcggaccga gggtaacttt cactgcatct
    12901 gccagtacgt ttacgccgcg cagcattttc acacgagcgt cgttaccgaa ttttacgtct
    12961 ttagctgcca ttatctttat tccttaaatt cgtatgttca gtgtcgtgcg aggattacgc
    13021 ttcaacaatt gccagaatgt cgctttcgga catgatcaac acttcttcat tgtcgatctt
    13081 ctcagatttc acaccgtagc catcgttgaa aataacgatg tcgccaactt tcacatccag
    13141 cggcttcact tcgccatttt caaggatacg gccattgccg acagccagca cttcgccgcg
    13201 ggtggattta gccgctgcag agccggtcag aacgatgccg ccagcagatt tagtttcaac
    13261 ttctttacgc ttgacgatca cgcgatcatg caatggacga atattcattg ataactctcc
    13321 tttgagaaag tccgtatctg ttatgggtga cgccggagct tacgtggttt cccggctggt
    13381 gaccagagaa atggggatga ggattcgccc cttcaagggg gaaaaagaaa aaaaattctg
    13441 atcagcacaa aatcgggtga aaaccctgat tcacctcaca tttcatcgca atttcttcat
    13501 cgccgtcata agcgaatctg attgtgctac catcgaaaat ctacgcagtt gcccaaaatt
    13561 tgggcgcaat cggatatcaa cccatgagtg gactcaaaca agaactgggg ctggcccagg
    13621 gcattggcct gctatcgacg tcattattag gcaccggcgt gtttgccgtt cctgcgttag
    13681 ctgcacaggt agcaggcaat aacagcctgt gggcgtggcc cgttttgatt atcttagtgt
    13741 tcccgattgc gattgtgttt gcgattctgg gtcgccacta tcccagcgca ggcggtgtcg
    13801 cgcacttcgt cggtatggcg tttggttcgc ggcttgagcg agtcaccggc tggttgtttt
    13861 tatcggtcat tcccgtgggt ttgcctgccg cgctacaaat tgccgccggg ttcggccagg
    13921 caatgtttgg ctggcatagc tggcaactgt tgttggcaga actcggtacg ctggcgctgg
    13981 tgtggtatat cggtactcgc ggtgccagtt ccagtgctaa tctacaaacc gttattgccg
    14041 gacttatcgt cgcgctgatt gtcgctatct ggtgggcggg cgatatcaaa cctgcgaata
    14101 tccccttccc ggcacctggt aatatcgaac ttaccgggtt atttgctgcg ttatcagtga
    14161 tgttctggtg ttttgtcggt ctggaagcat ttgcccatct tgcctcggaa tttaaaaatc
    14221 cagagcgtga ttttcctcgc gcattgatga ttggtctgct gctggcagga ttagtctact
    14281 ggggctgtac ggtagtcgtc ttacacttcg acgcctatgg tgaacaaatg gccgcggcag
    14341 catcgcttcc aaaaattgta gtgcaactgt tcggtgtagg agcgttatgg attgcctgcg
    14401 taattggcta tctgacctgc tttgccagtc tcaacattta tatacagagc ttcgcccgcc
    14461 tggtctggtc gcaggcgcaa cataatcctg accactacct ggcacgcctc tcttctcgcc
    14521 atatcccgaa taatgccctc aatgcggtgc tcggctgctg cgtggtgagc actttggtga
    14581 ttcatgcttt agagatcaat ctggacgctc ttattattta tgccaatggc atctttatta
    14641 tgatttatct gttatgcatg ctggcaggct gtaaattatt gcaaggacgt tatcgactac
    14701 tggcggtggt tggcgggctg ttatgcgttc tgttactggc aatggtcggc tggaaaagtc
    14761 tctatgcgct gattatgctg gcggggttat ggctgtttct gccaaaacga aaaacgccgg
    14821 aaaatggcat aaccacataa tccggcgttt cgacattaat cctagcgatc gtctttatga
    14881 tcaaggcggt cgcggtcatc atcctttcgc tggtactcac catcaaaagt attaccgcca
    14941 ccggtcccgg cgctaaaacc gccgcctggc atgcgagaaa agcgcaaatg tggcatcaac
    15001 ttcactgtca gatgcttttg caccggcggc aataaaagca gaagaccgag gaagtcggtg
    15061 aaaaagcccg gtaataaaag cagcaaaccg gcaataatca gcgaaacact tttaatcatc
    15121 tccgccgcag ggttttcacc cgccgccatt ttttgctgca tcagcacgaa attcttaaag
    15181 ccctggttac gtaccagtga cataccgata actgacgtga atataaccag cacgagggtc
    15241 agcaataccc ccaatacatg ggcaacctga ataaagattg aaatctcaat atagacataa
    15301 aggaaaatgg caataaaagg taaccagcgc aaaggtttct cctgtaatag cagccggtta
    15361 aacccggcta cctgaatggg ttgcgaatcg cgttcagctt atattgtggt cattagcaaa
    15421 atttcaagat gtttgcgcaa ctatttttgg tagtaatccc aaagcggtga tctatttcac
    15481 aaattaataa ttaaggggta aaaaccgaca cttaaagtga tccagattac ggtagaaatc
    15541 ctcaagcagc atatgatctc gggtattcgg tcgatgcagg ggataatcgt cggtcgaaaa
    15601 acattcgaaa ccacatatat tctgtgtgtt taaagcaaat cattggcagc ttgaaaaaga
    15661 aggttcacat gtcaaacaac attcgtatcg aagaagatct gttgggtacc agggaagttc
    15721 cagctgatgc ctactatggt gttcacactc tgagagcgat tgaaaacttc tatatcagca
    15781 acaacaaaat cagtgatatt cctgaatttg ttcgcggtat ggtaatggtt aaaaaagccg
    15841 cagctatggc aaacaaagag ctgcaaacca ttcctaaaag tgtagcgaat gccatcattg
    15901 ccgcatgtga tgaagtcctg aacaacggaa aatgcatgga tcagttcccg gtagacgtct
    15961 accagggcgg cgcaggtact tccgtaaaca tgaacaccaa cgaagtgctg gccaatatcg
    16021 gtctggaact gatgggtcac cagaaaggtg aatatcagta cctgaacccg aacgaccatg
    16081 ttaacaaatg tcagtccact aacgacgcct acccgaccgg tttccgtatc gcagtttact
    16141 cttccctgat taagctggta gatgcgatta accaactgcg tgaaggcttt gaacgtaaag
    16201 ctgtcgaatt ccaggacatc ctgaaaatgg gtcgtaccca gctgcaggac gcagtaccga
    16261 tgaccctcgg tcaggaattc cgcgctttca gcatcctgct gaaagaagaa gtgaaaaaca
    16321 tccaacgtac cgctgaactg ctgctggaag ttaaccttgg cgcaacagca atcggtactg
    16381 gtctgaacac gccgaaagag tactctccgc tggcagtgaa aaaactggct gaagtcactg
    16441 gcttcccatg cgtaccggct gaagacctga tcgaagcgac ctctgactgc ggcgcttatg
    16501 ttatggttca cggcgcgctg aaacgcctgg ctgtgaagat gtccaaaatc tgtaacgacc
    16561 tgcgcttgct ctcttctggt ccacgtgccg gcctgaacga gatcaacctg ccggaactgc
    16621 aggcgggctc ttccatcatg ccagctaaag taaacccggt tgttccggaa gtggttaacc
    16681 aggtatgctt caaagtcatc ggtaacgaca ccactgttac catggcagca gaagcaggtc
    16741 agctgcagtt gaacgttatg gagccggtca ttggccaggc tatgttcgaa tccgttcaca
    16801 ttctgaccaa cgcttgctac aacctgctgg aaaaatgcat taacggcatc actgctaaca
    16861 aagaagtgtg cgaaggttac gtttacaact ctatcggtat cgttacttac ctgaacccgt
    16921 tcatcggtca ccacaacggt gacatcgtgg gtaaaatctg tgccgaaacc ggtaagagtg
    16981 tacgtgaagt cgttctggaa cgcggtctgt tgactgaagc ggaacttgac gatattttct
    17041 ccgtacagaa tctgatgcac ccggcttaca aagcaaaacg ctatactgat gaaagcgaac
    17101 agtaatcgta cagggtagta caaataaaga aggcacgtca gatgacgtgc cttttttctt
    17161 gtgagcagta acttaaaaat aacaatctaa tatcaacttg ttaaaaaaca aggaaggcta
    17221 atatgctagt tgtagaactc atcatagttt tgctggcgat cttcttgggc gccagattgg
    17281 ggggaatagg tattggtttt gcaggcggat tgggggtgct ggttcttgcc gctattggcg
    17341 ttaaacccgg taacatcccg ttcgatgtca tttccattat catggcggtt atcgccgcta
    17401 tttctgccat gcaggttgct ggcggtctgg actatctggt tcatcagaca gaaaagctgc
    17461 tgcgccgtaa cccgaaatac atcacaatcc tcgcaccgat cgtgacctat ttcctgacaa
    17521 tctttgctgg tactggcaac atctctctgg cgacactgcc agttatcgct gaagttgcga
    17581 aggaacaagg cgttaaacct tgccgtccgc tgtctactgc agtggtatcc gcgcagattg
    17641 cgatcaccgc atcgccaatc tcagcggcag tggtttacat gtcttccgtg atggaaggtc
    17701 atggcatcag ctacctccat ctgctctccg tggtcatccc gtccaccctg ctggcggttc
    17761 tggtgatgtc cttcctggtc actatgctgt tcaactccaa actctctgac gatccgattt
    17821 atcgcaagcg tctggaagag ggcctggttg aactgcgcgg tgaaaagcag attgaaatca
    17881 aatccggtgc aaaaacgtcc gtctggctgt tcctgctggg cgtagttggc gtggttatct
    17941 atgcaatcat caacagccca agcatgggtc tggttgaaaa accgctgatg aacaccacca
    18001 acgcaatcct gatcatcatg ctcagcgttg caactctgac caccgttatc tgtaaagtcg
    18061 ataccgacaa catcctcaac tccagcacct tcaaagcagg tatgagcgcc tgtatttgta
    18121 tcctgggtgt tgcgtggctg ggcgatactt tcgtttccaa caacatcgac tggatcaaag
    18181 ataccgctgg tgaagtgatt cagggtcatc cgtggctgct ggccgtcatc ttcttctttg
    18241 cttctgctct gctgtactct caggctgcaa ccgcaaaagc actgatgccg atggctctgg
    18301 cactgaacgt ttctccgctg accgctgttg cttctttcgc tgcggtgtct ggtctgttca
    18361 ttctgccgac ctatccgacg ctggttgctg cggtacagat ggatgacacg ggtacaaccc
    18421 gtatcggtaa attcgtcttc aaccatccgt tcttcatccc gggtactctg ggtgttgccc
    18481 tggccgtttg cttcggcttc gtgctgggta gcttcatgct gtaatgacca atcgcggggc
    18541 gttcacgccc cgctttcttt cccgccgact aacatccttc ccccgtccgt tgtatagtga
    18601 cctctctctt gcggttccat ctgttcttgc gaggtgttta tgcttgatga aaaaagttcg
    18661 aataccgcgt ctgtcgtggt gctatgtacc gcaccggatg aagcgacagc ccaggattta
    18721 gccgccaaag tgctggcgga aaaactggcg gcctgcgcga ccttgatccc cggcgctacc
    18781 tctctctatt actgggaagg taagctggag caagaatacg aagtgcagat gattttaaaa
    18841 actaccgtat ctcaccagca ggcactgctg gaatgcctga agtctcatca tccatatcaa
    18901 accccggaac ttctggtttt acctgttaca cacggagaca cagattacct ctcatggctc
    18961 aacgcatctt tacgctgatc ctgctacttt gcagcacttc cgtttttgcc ggattattcg
    19021 acgcgccggg acgttcacat tttgtccccg cggatcaagc ctttgctttt gattttcagc
    19081 aaaaccaaca tgacctgaat ctgacctggc agatcaaaga cggttactac ctctaccgta
    19141 aacagatccg cattacgccg gaacacgcga aaattgccga cgtgcagctg ccgcaaggcg
    19201 tctggcatga agatgagttt tacggcaaaa gcgagattta ccgcgatcgg ctgacgcttc
    19261 ccgtaaccat caaccaggcg agtgcgggag cgacgttaac tgtcacctac cagggctgtg
    19321 ctgatgccgg tttctgttat ccgccagaaa ccaaaaccgt tccgttaagc gaagtggtcg
    19381 ccaacaacgc agcgtcacag cctgtgtctg ttccgcagca agagcagcac accgcgcaat
    19441 tgcccttttc cgcgctctgg gcgttgttga tcggtattgg tatcgccttt acgccatgcg
    19501 tgctgccaat gtacccactg atttctggca tcgtgctggg tggtaaacag cggctctcca
    19561 ctgccagagc attgttgctg acctttattt atgtgcaggg gatggcactg acttacacgg
    19621 cgctgggtct ggtagttgcc gccgcagggt tacagttcca ggcggcgcta cagcacccat
    19681 acgtgctcat tggcctcgcc atcgtcttta ccttgctggc gatgtcaatg tttggcttgt
    19741 ttactctaca actcccctct tcgctgcaaa cacatctcac attgatgagc aatcgccaac
    19801 agggcggctc acctggcggt gtgtttgtta tgggggcgat tgccggactg atctgttcac
    19861 catgcaccac cgcaccgctt agcgcgattc tgctgtatat cgcccaaagc gggaacatgt
    19921 ggctgggcgg cggcatgctt tatctctatg cgttgggcat gggcctgccg ctgatgctaa
    19981 ttaccgtctt tggtaaccgc ttgctgccga aaagcggccc gtggatggaa caagtcaaaa
    20041 ccgcgtttgg ttttgtgatc ctcgcactgc cgatcttcct gctggagcga gtgattggtg
    20101 atatatgggg attacgcttg tggtcggcgc tgggtgtcgc attctttggc tgggccttta
    20161 tcaccagcct acaggccaaa cgcggctgga tgcgcgtggt gcaaataatc ctgctggcag
    20221 cggcattggt tagcgtgcgc ccacttcagg attgggcatt tggtgcgacg cataccgcgc
    20281 aaactcagac gcatctcaac tttacacaaa tcaaaacggt agatgagtta aatcaggcgc
    20341 tcgttgaagc caaaggcaaa ccggtgatgt tagatcttta tgccgactgg tgcgtcgcct
    20401 gtaaaaagtt tgagaaatac accttcagcg acccgcaggt gcaaaaagcg ttagcagaca
    20461 cggtcttact tcaggccaac gtcactgcta acgacgcaca agatgtggcg ctgttaaagc
    20521 atcttaatgt ccttggacta ccgacaattc tcttttttga cggacaaggc caggagcatc
    20581 cacaagcacg cgtcacgggc tttatggatg ctgaaacctt cagcgcacat ttgcgcgatc
    20641 gccaaccgtg aacgacactt gcagtgggaa agacggagga aaatagcgtg caacgtgaag
    20701 atgtactggg agaagccctg aaattattag aattacaagg gattgccaac accacgctgg
    20761 agatggttgc tgaacgtgtg gattatccac tggacgagct acgccgcttc tggccagaca
    20821 aagaggcaat cctctacgat gcgctgcgtt atcttagcca acagatagat gtctggcgtc
    20881 gtcagctgat gcttgacgaa acgcaaaccg ccgaacaaaa gctgctggca cgttatcagg
    20941 cgttatcgga gtgcgttaaa aacaaccgct atccgggctg tctatttatc gctgcctgta
    21001 cgttttatcc cgaccctggc caccctattc atcaactggc cgatcagcaa aaaagcgcgg
    21061 cctacgattt cacccacgaa ctgttaacca cgctggaagt tgacgatccg gcgatggtag
    21121 caaagcagat ggaactggtg ctggaaggct gtttaagccg aatgctggtg aatcgtagcc
    21181 aggcggatgt cgacaccgca catcggctgg cggaagatat cctgcgcttc gcccgctgcc
    21241 gccagggtgg tgcactgacc tgacagaaac acagaaaaga agcgatttgc cgcaatctta
    21301 agcagttgaa tcgcttttac tgaaattagg ttgacgagat gtgcagatta cggtttaatg
    21361 cgccccgttg cccggatagc tcagtcggta gagcagggga ttgaaaatcc ccgtgtcctt
    21421 ggttcgattc cgagtccggg caccaaattc atataaacgg acctccacgg aggtccgttt
    21481 ttcgtttcag gacgccacga tttaagcgtt ctgcctccaa atcaattcta ccgaactcaa
    21541 ccagattctc cccacatcac cagcattttg cgggtatatc ccaattcggg aaaatttgtt
    21601 cctgagcgat agcactgact gacgtgaaat gtcgtgcggc cccgtgatgt tgttgaacgt
    21661 caaatgacgt catcaggagc gtaacgcatc cataaagcac aacaccggtc tgaacggcga
    21721 ctgatgagat tttctgaatg agaacaaaga gaaatgtatc agtccgcttg ctcatgcaaa
    21781 gactaacaat ccattaaaat agtaagcgct ccggacaatt ttccatggat tattttctac
    21841 acatttttct ttggcaaaga tgatgaattt tgatggtaag gaatattact tctggttctc
    21901 agtaaaatcc tttcgtaata ctatgtaatc aagaagttta tggctggtaa aaataacgtc
    21961 ttgcattcac caataacatg taaataaacc catctattga tggtaaaaat aggtcatggc
    22021 aattatcatt gcaccattcc cttttcgaat gagtttctat tatgcaacaa cctgtagttc
    22081 gcgttggcga atggcttgtt actccgtcca taaaccaaat tagccgcaat gggcgtcaac
    22141 ttacccttga gccgagatta attgatcttc tggttttctt tgctcaacac agtggcgaag
    22201 tacttagcag ggatgaactt atcgataatg tctggaagag aagtattgtc accaatcacg
    22261 ttgtgacgca gagtatctca gaactacgta agtcattaaa agataatgat gaagatagtc
    22321 ctgtctatat cgctactgta ccaaagcgcg gctataaatg aatggtgccg gttatctggt
    22381 acagcgaaga agagggagag gaaataatgc tatcttcgcc tccccctata ccagaggcgg
    22441 ttcctgacac aaattctccc tcccacagtc ttaccattca aaacaccgca acgccacctg
    22501 aacaatcccc agttaaaagc aaacgattca ctaccttttg ggtatggttt ttttcctgtt
    22561 gtcgttaggt atctgtgtag cactggtagc gttttcaact cttgatacac gtcttcctat
    22621 gagcaaatcg cgtattttgc tcaatccacg cgatattgac attaatatgg taaataaaag
    22681 ttgtaacagc tggagttccc cgtatcagct ctcttacgcg ataggcgtgg gtgatttggt
    22741 ggcgacatca cttaacacct tctccacctt tatggtgcat gacaaaatca actacaacat
    22801 tgatgaaccg agcagttccg gtaaaacatt atctattgcc tttgttaatc agcgccaata
    22861 ccgtgctcaa caatgcttta tgtcggtaaa attagtagac aatgcagatg gttcaaccat
    22921 gctggataaa cgttatgtca tcactaacgg taatcagctg gcgattcaaa atgatttact
    22981 ggagagttta tcaaaagcgt taaaccaacc gtggccacaa cgaatgcagg agacgctcca
    23041 gcaaattttg cctcatcgtg gtgcgttatt aactaatttt tatcaggctc atgattattt
    23101 actgcatggc gatgacaaat cattgaatcg tgccagtgaa ttattaggtg agattgttca
    23161 atcatcccca gaatttacct acgcgagagc agaaaaagca ttagttgata tcgtgcgcca
    23221 ttctcaacac cctttagatg aaaaacaatt agcagcactg aacacagaaa tagacaacat
    23281 tgttacactg ccggaattga acaacctgtc cattatatat caaataaaag cggtcagtgc
    23341 tctggtaaaa ggtaaaatag atgagtctta ccaggcgata aatactggca ttgatcttga
    23401 aatgtcctgg ctaaattatg tgttgcttgg caaggtttat gaaatgaagg ggatgaaccg
    23461 ggaagcagct gatgcatatc tcactgcctt taatttacgc ccaggggcaa acacccttta
    23521 ctggattgaa aatggtattt tccagacttc tgttccttat gttgtacctt atctcgacaa
    23581 atttctcgct tcagaataag taactccggg ttgatttatg ctctggaata tttgttgttg
    23641 agtttttgta tgttcccatt ggtataatat gttgcggcaa tttatttgcc gcataatttt
    23701 tattacataa atttaaccag agaatgtcat gcaatccatt gtaaacattg aatgtttatc
    23761 ttttcatgat atcaacttgc gatcctgatg tgttaataaa aaacctcaag ttctcactta
    23821 cagaaacttt tgtgttattt cacctaatct ttaggattaa tccttttttc gtgagtaatc
    23881 ttatcgccag tttggtctgg tcaggaaata gttatacatc atgacccgga ctccaaattc
    23941 aaaaatgaaa ttaggagaag agcatgagtt ctgccaagaa gatcgggcta tttgcctgta
    24001 ccggtgttgt tgccggtaat atgatgggga gcggtattgc attattacct gcgaacctag
    24061 caagtatcgg tggtattgct atctggggtt ggattatctc tattattggt gcaatgtcgc
    24121 tggcgtatgt atatgcccga ctggcaacaa aaaacccgca acaaggtggc ccaattgctt
    24181 atgccggaga aatttctcct gcatttggtt ttcagacagg tgttctttat taccatgcta
    24241 actggattgg taacctagcg attggtatta ccgctgtatc ttatctttcc accttcttcc
    24301 cagtattaaa tgatcctgtt ccggcgggta tcgcctgtat tgctatcgtc tgggtattta
    24361 cctttgtaaa tatgctcggc ggtacctggg taagccgttt aaccactatt ggtctggtgc
    24421 tggttcttat tcctgtggtg atgactgcta ttgttggctg gcattggttt gatgcggcaa
    24481 cttatgcagc taactggaat actgcggata ccactgatgg tcatgcgatc attaaaagta
    24541 ttctgctcta cctgtgggcc ttcgtgggtg ttgagtccgc agcagtaagt actggtatgg
    24601 ttaaaaaccc gaaacgtacc gttccgctgg caaccatgct gggtactggt ttagcaggta
    24661 ttgtttacat cgctgcgact caggtgcttt ccggtatgta tccgtcttct gtaatggcgg
    24721 cttccggtgc tccgtttgca atcagtgctt caactatcct cggtaactgg gctgcgccgc
    24781 tggtttctgc attcaccgcc tttgcgtgcc tgacttctct gggctcctgg atgatgttgg
    24841 taggccaggc aggtgtacgt gccgctaacg acggtaactt cccgaaagtt tatggtgaag
    24901 tcgacagcaa cggtattccg aaaaaaggtc tgct
//
LOCUS       DAZPDU010000049        24816 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZPDU010000049 DAZPDU010000000
VERSION     DAZPDU010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24816
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.049"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(87..377)
                     /gene="ubiK"
                     /locus_tag="ACHURS_001811"
     CDS             complement(87..377)
                     /gene="ubiK"
                     /locus_tag="ACHURS_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFR1237563.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLARLEQRMSELENRSTEIKKQPDPETLPPTL"
     regulatory      499..647
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            751..1404
                     /gene="ribB"
                     /locus_tag="ACHURS_001812"
     CDS             751..1404
                     /gene="ribB"
                     /locus_tag="ACHURS_001812"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFR1237564.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     gene            1665..1835
                     /gene="yqiD"
                     /locus_tag="ACHURS_001813"
     CDS             1665..1835
                     /gene="yqiD"
                     /locus_tag="ACHURS_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFR1237565.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(1893..2666)
                     /gene="zupT"
                     /locus_tag="ACHURS_001814"
     CDS             complement(1893..2666)
                     /gene="zupT"
                     /locus_tag="ACHURS_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFR1237566.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            2809..3597
                     /gene="ygiD"
                     /locus_tag="ACHURS_001815"
     CDS             2809..3597
                     /gene="ygiD"
                     /locus_tag="ACHURS_001815"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFR1237567.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLASVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            complement(3635..4795)
                     /locus_tag="ACHURS_001816"
     CDS             complement(3635..4795)
                     /locus_tag="ACHURS_001816"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFR1237568.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKRTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFIE"
     gene            complement(4801..5472)
                     /locus_tag="ACHURS_001817"
     CDS             complement(4801..5472)
                     /locus_tag="ACHURS_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFR1237569.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            complement(5620..7101)
                     /gene="tolC"
                     /locus_tag="ACHURS_001818"
     CDS             complement(5620..7101)
                     /gene="tolC"
                     /locus_tag="ACHURS_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFR1237570.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            7306..7935
                     /gene="nudF"
                     /locus_tag="ACHURS_001819"
     CDS             7306..7935
                     /gene="nudF"
                     /locus_tag="ACHURS_001819"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFR1237571.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            7936..8358
                     /locus_tag="ACHURS_001820"
     CDS             7936..8358
                     /locus_tag="ACHURS_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFR1237572.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            8383..9210
                     /gene="cpdA"
                     /locus_tag="ACHURS_001821"
     CDS             8383..9210
                     /gene="cpdA"
                     /locus_tag="ACHURS_001821"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFR1237573.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            9210..9791
                     /gene="yqiA"
                     /locus_tag="ACHURS_001822"
     CDS             9210..9791
                     /gene="yqiA"
                     /locus_tag="ACHURS_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFR1237574.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            9820..11712
                     /gene="parE"
                     /locus_tag="ACHURS_001823"
     CDS             9820..11712
                     /gene="parE"
                     /locus_tag="ACHURS_001823"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFR1237575.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(11760..12074)
                     /locus_tag="ACHURS_001824"
     CDS             complement(11760..12074)
                     /locus_tag="ACHURS_001824"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFR1237576.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(12105..12686)
                     /gene="mdaB"
                     /locus_tag="ACHURS_001825"
     CDS             complement(12105..12686)
                     /gene="mdaB"
                     /locus_tag="ACHURS_001825"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFR1237577.1"
                     /translation="MSNTLIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            complement(12796..14145)
                     /gene="qseC"
                     /locus_tag="ACHURS_001826"
     CDS             complement(12796..14145)
                     /gene="qseC"
                     /locus_tag="ACHURS_001826"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFR1237578.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHSIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            complement(14142..14801)
                     /gene="qseB"
                     /locus_tag="ACHURS_001827"
     CDS             complement(14142..14801)
                     /gene="qseB"
                     /locus_tag="ACHURS_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFR1237579.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            14953..15345
                     /gene="ygiW"
                     /locus_tag="ACHURS_001828"
     CDS             14953..15345
                     /gene="ygiW"
                     /locus_tag="ACHURS_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFR1237580.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            15398..15880
                     /locus_tag="ACHURS_001829"
     CDS             15398..15880
                     /locus_tag="ACHURS_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFR1237581.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            16426..18684
                     /gene="parC"
                     /locus_tag="ACHURS_001830"
     CDS             16426..18684
                     /gene="parC"
                     /locus_tag="ACHURS_001830"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFR1237582.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQSVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASNGDSEE"
     gene            18917..19654
                     /gene="plsC"
                     /locus_tag="ACHURS_001831"
                     /gene_synonym="parF"
     CDS             18917..19654
                     /gene="plsC"
                     /locus_tag="ACHURS_001831"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFR1237583.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            19729..21141
                     /gene="ftsP"
                     /locus_tag="ACHURS_001832"
     CDS             19729..21141
                     /gene="ftsP"
                     /locus_tag="ACHURS_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFR1237584.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYDVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            21252..23471
                     /locus_tag="ACHURS_001833"
     CDS             21252..23471
                     /locus_tag="ACHURS_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFR1237585.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCVRALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRPALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(23514..23771)
                     /gene="yqhH"
                     /locus_tag="ACHURS_001834"
     CDS             complement(23514..23771)
                     /gene="yqhH"
                     /locus_tag="ACHURS_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFR1237586.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDIKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(23822..24748)
                     /locus_tag="ACHURS_001835"
     CDS             complement(23822..24748)
                     /locus_tag="ACHURS_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFR1237587.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
BASE COUNT         5802 a         6235 c         6643 g         6136 t
ORIGIN      
        1 ctgaatggaa ttaagcgata aggtaataga aagcactgta aattagaaga ttaattcata
       61 atttacagtg cattaaagac aaatgtttac agcgttgggg ggagagtctc tggatctggc
      121 tgctttttaa tctcagtact gcgattttcc agttcgctca ttctttgttc aagccgcgcc
      181 agtttctcac gagtccgtaa caggacttgc gtttggacgt cgaactcttc acggcttaca
      241 aggtcgaggc gcgtcagctg agcttgtagg gtttggcgga tttttttctc cacatcttcc
      301 ccgaactccc tgattccttt aggcattgat tcgtgaacct ggcgagcgat ttgctcaatt
      361 tttttcgggt caatcattgt agtttccctg tactgatagg tgttgagtac cattgtagtg
      421 cgataagggt aagtcataaa ccagaattat gtgaagctat gcgttgctgc cgctaatcat
      481 tagcgttata gtgaatccgc ttattctcag ggcggggcga aattccccac cggcggtaaa
      541 tcaactcagt tgaaagcccg cgagcgcttt gggtgcgaac tcaaaggaca gcagatccgg
      601 tgtaattccg gggccgacgg ttagagtccg gatgggagag agtaacgatt ctgtcgggca
      661 tggacccgct cacgttattt tggctatatg ccgccactcc taagactgcc ctgattctgg
      721 taaccataat tttagtgagg tttttttacc atgaatcaga cgctactttc ctcttttggt
      781 acgcctttcg aacgtgttga aaatgcactg gctgcgctgc gtgaaggacg cggtgtaatg
      841 gtgcttgatg atgaagaccg tgaaaacgaa ggcgatatga tcttcccggc agaaaccatg
      901 accgttgagc agatggcgct gaccattcgc cacggtagcg gtattgtttg cctgtgcatc
      961 actgaagatc gccgtaaaca acttgatctg ccaatgatgg tagaaaataa caccagcgcc
     1021 tatggcaccg gttttaccgt gaccattgaa gcggctgaag gtgtgactac cggtgtttct
     1081 gccgctgatc gtattacgac cgttcgcgca gcgattgccg atggcgcaaa accttctgat
     1141 ctgaatcgcc cgggccacgt tttcccactt cgcgcgcagg caggtggtgt actgacgcgt
     1201 ggcggtcata ctgaagcgac tattgatttg atgacgctgg caggctttaa accggctggt
     1261 gtgctgtgtg agctgactaa tgacgatggc acgatggcgc gtgcaccaga gtgcattgag
     1321 tttgccaata aacacaatat ggcgctcgtg actattgaag acctggtggc ataccgtcag
     1381 gcacatgagc gtaaagccag ctgaaaaccg ctgcttaatt actgccttaa tcaagaaacc
     1441 gaagttgtag ctggcttcgg tttttatttt gccctgctat gcaagatgtt aattccgctt
     1501 ctctttctat gagaaaattt cattaatatc aggcattctt tttcattatg atcaaaatca
     1561 tataattgtt ccgttattat gcgaccatat gaatatatcc gaaaaaggat atgtgaaatt
     1621 tatgcgctaa atgtttagta ttgttaaata ttaagggtaa aaggatgttt atcgcctggt
     1681 actggattgt attgattgct ctggttgtgg tgggttattt cctgcatttg aaacgttatt
     1741 gtcgggcgtt tcgccaggac agagacgcac tgcttgaagc gcggaacaaa tacttaaaca
     1801 gtacgagaga agagacagcc gaaaaggtag aataaggctt tcgtgaagct ggcagaagtt
     1861 gtccatgtgc tggacaactt ctgatgttgt ttttaaccaa ttcccgccgt ttgtagcagc
     1921 actaaactga atcccatcac tgacattcca catagtacgc catagctggg gttattatta
     1981 gggtcaatct ctttggcgag cggcattaat tcatcaaccg agagcgccac cataattcct
     2041 gcaaccgccg ccatgattgc cgccatgacc accggggaaa tcatgctacc gaggattaac
     2101 cacgccagca caccaccaag aatttctgct agtccagaaa tccccgccca cagaatcgcg
     2161 gtgcgtttag accccgttgc cgcataaacc gggcctgcca ctgccagacc ttcagggata
     2221 ttgtgcaacg cgacggccag tgcgatgcca aatcccagct ccaggttgct gctcgccgtg
     2281 acaaaggtgg caatcccttc cgggaagtta tgcagactga tgccgagagt gagcagaatg
     2341 gctgtgcgct tgatcgattt tggcaacggc tgcaccgatt tttgcattaa atcctgcgga
     2401 tgcgcatgtg gcaacatgcg gtccaggcca aaatagccaa gcagaccgaa gataaacatt
     2461 ccataaccca acacaggcga cattccttca gctgctagtg cggcaggaag catttccatt
     2521 aatgagatga gcaacatgat ccccgccgca aatcctagcg aaaacgccag taagcggttc
     2581 gagggttttt gcccgagaac gccgagaaac gcgccaataa acgtggctgc ccccgccagt
     2641 atggtcagaa tgagaggtac tgacatcgac aactccttat aaatcttcac cttcaggcag
     2701 atcgtcaaaa taactgatga tcatcatcat tgttatccgg gttcttactc cgccgaacaa
     2761 gcgtattgtg aggatatcaa aatgacacct ttagttaagg atatcatcat gtcttcaaca
     2821 cgtatgccag cattgttttt aggtcacggt agtccgatga acgtgctgga agataatttg
     2881 tatacccgca gctggcagaa gttggggatg acattgccac gcccgcaagc gattgtggtg
     2941 gtttcggctc actggtttac ccgtggaaca ggagtgaccg cgatggagac gccgcccacg
     3001 attcatgact ttggtggctt cccgcaggcg ctgtacgata cgcattatcc tgctccgggt
     3061 tcgcctgtgc tggcacagca tctggttgag ctgttagcgt cggtcccggt gacgctggat
     3121 aaagaagcct ggggctttga ccacggttcc tggggcgtgc tgattaagat gtatcctgac
     3181 gccgatatcc cgatggtgca gttgagtatc gacagtagca aacctgccgc ctggcatttc
     3241 gaaatggggc gcaaactggc agcgctgcga gatgaaggaa taatgttggt cgccagtggc
     3301 aacgtggtgc ataacctgcg tacagtgaag tggcacggtg atagttcacc atatccttgg
     3361 gcgatgtcgt ttaatgagta tgtgaaagcg aatctgacgt ggcaaggggc ggtagagcag
     3421 catcctctgg tgaattacct tgaccatgaa ggtggcgcgt tatcgaaccc aacgccggag
     3481 cactatctgc cgttgttgta tgtcttaggt gcgtgggatg ggcaggagcc aattaccatt
     3541 ccggtcgatg gtatagaaat gggcagcctg agtatgctgt cggtgcagat aggttaacaa
     3601 ttaccggatg gcgaatgcca tccggcatca ttgattactc aataaaaata tgtggataaa
     3661 accgagacat atcctgggtg atcaacgcac ggtcttcacg aatgccaatt ccggcaggtt
     3721 gatcgttcac cagccagcta ccaatcagca tatagctgtc gccgaatttc ggtaacgggt
     3781 ggaattgctg aacaatcatc ccttcttcgc catacggacc ttccgctgct tcaatggttt
     3841 tgccgttctc aatgatcgac acgtttgcgc cttcacggga gaagatcggt ttaaccacat
     3901 atttttccat ttgcggatga tcatcttccg caaaataagc gggcagcagg ttcgggtgat
     3961 tcgggaacat ctcccacagt agcggtagaa gcgctttgtt ggagataatg ctcttccacg
     4021 ccggttccag ccagcgtacg cctgcatcct ccagcttggt ggagaacatc tcacgcaaca
     4081 taaattccca tggatacagc ttgaacaggt tggaaattac ctgatcctgt aaatccgtga
     4141 actgaccttt ttcacctaac ccgatatcgt caatgtagag gaactcggta gcgatttcag
     4201 cttccgtcgc gcagtcctgc aaatactgaa tggttccgcg atcttccacc gtgtcgcgac
     4261 agcaggtgag atgcagcaac tggaagccat actgttcacg caactcgaca aagcgatcga
     4321 ttagcttttc ttgcagactg ttaaactggt cgctgccctc cggcaagtta ccggcgttaa
     4381 gctgatcttc cagccagatc cactgaaaga acgccgcctc gtatagtgac gttggtgtat
     4441 cggcgttatt ttccagcagt ttaggttcgc cagtgccatc ccatgccaga tcaagacgcg
     4501 aataaagcga tggctggtga gtcagccatg actgacgcac aaaactccag gtgcgttttg
     4561 gaatgcggaa tttggtcatc agctcatcgc tggcgatcac tttttccacc actttcaggc
     4621 acatctggtg cagttcggcg gtgacttctt ccagcttttc aacctgggcg agggtcaact
     4681 tgtagtaagc atcttcacac cagtacggct cgccgtacat ggtgtgaaaa ttgaaaccgt
     4741 attcgtgggc tttttcgcgc cagtccgggc gctcggtaat actgactctt tccatcggta
     4801 tcagccaccc attgaacgag aagaggtgcc ggttgcgcta cgctgcatag tgctttgttt
     4861 ggcaacagat tcaccaaaac cgccacgggt aacggtagta gtggtcgccg gttttggtgc
     4921 catggctgtc ttcggtacgg tcatggtgcg gcctggctgg gctgcgccat agtttttacc
     4981 cgtcgcgtcg gtatatttac cgtaagccgg gctggctggg tttttcgagg agaacagcgg
     5041 ctgctgtgca aatcccgcgc cgccgcccat cagacgcccc atcatgtaac cggccatcag
     5101 cggcatccag aaactcccgc tggattgctg ggcctgcgcc tggttttctg gtgccatgcc
     5161 agcctgggct ggtgcctgct ggcactgacc ttcaccaaat tcagcaacac agtcttcacg
     5221 ggtggcgtat ttcggcgcag tacgttcggc ttctttcagc gcattgttgt acgcggtggt
     5281 acattcggcg cttttgcctg ggtttgcagc tgaacagtcg tcagcatttt gatagagaga
     5341 cactgtttca tcactctttt cacagccagc cagcataaaa acagtggcaa ccgcgagagc
     5401 gactggtgtc agatggcgtg cgctccagtt tttgcggaac gatgcgtggc gtatggattt
     5461 tgtccgtttc atttttgtct tccgggacca gtggtaaata cccatcagaa tagaggatgg
     5521 ctggtcgaaa ttgaagcgag aagggggaag aatgcggcag ataacccgta tctttacgtt
     5581 gccttacgtt cagacggggc cgaagccccg tcgtcgtcgt cagttacgga aagggttatg
     5641 accgttactg gtggtagtgc gtgcggatgt ttgctgaacg actggtgccg ggctatcagg
     5701 cgcataacca tcagcaatag cattctgttc cggcgtttgc ggtgcaacgt tttccggatt
     5761 agtggaaacc ggtttgctca gcgcattgtt cagtgccagc agatcctgct cgttcaacgt
     5821 acccagagct gacttaatat tcaactggtt aatcaggtag ttataacgcg catttgccag
     5881 ctcttgctta gcgttgtaca gcgtggtggt tgcatccaac acatcaacaa tggtacgcgt
     5941 accgaccgag tagcccgctt ccatcgcgtc taatgagctt tgagcggaaa ctacggcttg
     6001 tttgtaggcg ttaatgctac tgatagatgc attaatgttg ttgaaggagg aacgtacggt
     6061 ttgcacgacg ctacgatgcg cgctttccag ttgctcgctg gcaccaacaa agttgtactg
     6121 ggcctgtttc acctgcgagt taaccattcc gccctgataa atcggcagcg agaagctcag
     6181 gcccactttg ttctggccca tattgctgtc gtcatactgg gtaccagcgg caccacgggt
     6241 tttcgaaccg ctataagagg tgtcagaaat cccggtagaa gccgttaaat ccagcgtcgg
     6301 taagtgacca tcctgcgcct ggcgaatttg ctcgcgcgcc aggtcctggc tcaagcgtgc
     6361 ctgtaacagc gacaggttgc gtttttcggc ttctttcagc agcgcgttaa ccggctgtgg
     6421 tttgtcggtt ttaaagtttt cgacattcag cgccgccagt tccggatagt agttaccggt
     6481 gatctggcgc agctgctcta ccgcgttatc aaggttatta cgtgcggtca cttcgttcgc
     6541 cagcacggta tcgtactgcg cgcgggcgtt ctgcacgtcg gtgatcgcta ccaggcccac
     6601 gttaaaacgt tgggtggttt gatctaattg acggtagatc gcttcttttt gtgcctgtgt
     6661 ataggaaaga acgtcaatag cattcaacac gttgaaataa gcggtcgcgg tgttgaggat
     6721 caaggtttgc tgatcggtct gatatgtgac gtcctgaatc cctgctgctt tttcctgcag
     6781 cgttaacgca cgccatttcg acatatcaaa aatggattga gttaactgca gggacgcact
     6841 ggtcgcgtta gagttgatgc cgttcgcgtc gcggtagccg ttgctatagg tgtaatctgc
     6901 acctaaacct agctgtggca gtaatggact gcgcgcttca ttaatttttt caaaggcagc
     6961 atcacgatcg gcggcagact tacgcaattc cgggttacta aggcgtgctt gctgataaac
     7021 ttgcatcagg ttctcggcct ggctcaacga actgaaccca gaaaggctca ggccgataag
     7081 aatggggagc aatttcttca tttgcattcc ttgtggtgaa gcagtattta gcgcgatcaa
     7141 actgtaaaat tattgcggat tctagcagaa gccgctaccg caaaaggttg gcgttacgtg
     7201 ccatagggcg ttaatgtgcc tcaatttaac acatgactgc tcaaacggca gtcaaacgtc
     7261 gctgaaattc acatttaatt cactattagt gccaggacat taacaatgct taagccagac
     7321 aacctgcccg ttacatttgg caaaaacgat gtagaaatta ttgcacgaga aacactttat
     7381 cgcggctttt tttcattaga tctttataga tttcgtcatc gtctattcaa cgggcaaatg
     7441 agtcatgagg tacggcggga aatttttgag cgcggtcacg ccgcagtctt actacccttt
     7501 gacccagtgc gcgatgaagt tgtgctgatt gagcagattc ggattgccgc atacgacacc
     7561 agtgaaaccc cctggctact ggagatggtt gccgggatga tagaagaggg tgaaagtgtg
     7621 gaagatgttg cccgtcgcga agcgattgaa gaggcgggac tgatagtcaa acggaccaaa
     7681 ccggtgttaa gtttcctggc aagcccgggg ggcaccagtg agcgttcgtc aattatggtg
     7741 ggcgaagtgg acgccacgac cgcaagcggt attcatggtc tggctgatga aaacgaagat
     7801 attcgcgttc atgtggtaag ccgggaacag gcataccagt gggtagaaga ggggaaaatc
     7861 gacaacgcag cgtcggtcat cgctttgcaa tggctgcagc tgcatcatca agcgttaaaa
     7921 aatgagtggg cataaatgaa gcgttacaca cctgactttc ctgaaatgat gcgcctgtgc
     7981 gagatgaact tttcacaatt gcgccgtttg ttaccgcgca atgacgcacc cggcgaaact
     8041 gtaagctatc aggtggcaaa cgcacaatat cggctgacga ttgtggaatc gacccgatac
     8101 actaccctgg tgacaataga acagactgcg cccgcgatca gttactggag ccttccgtca
     8161 atgacggtgc gtctgtatca tgacgcgatg gtggctgaag tgtgttcaag ccagcagatt
     8221 tttcgcttca aagcgcggta tgattatcct aataaaaagt tgcatcaacg cgacgaaaag
     8281 catcaaatta atcagttttt agcggactgg ttgcgatact gtttagcaca tggagcgatg
     8341 gcgattccgg tttactagca tcgtgaaacc taaggacacc atttggaaag cctgttaacc
     8401 cttcctctgg ctggtgaggc cagagtcagg attttacaaa ttaccgacac tcacctgttt
     8461 gcacaaaagc acgaagctct gttaggggta aacacctggg aaagctacca ggcggtgctg
     8521 gaggcgattc gtccacatca gcacgaattc gacctgattg tcgcgacagg tgatttagcg
     8581 caggatcaat cctctgcggc ctatcagcat ttcgctgaag gcatcgcaag ttttcgtgcg
     8641 ccctgcgtct ggctgccggg caaccacgat ttccagcccg cgatgtacag cgcgttacag
     8701 gatgcgggta tctccccggc gaagcgggtg tttattggtg agcaatggca aatcctgttg
     8761 ctggatagcc aggtgtttgg cgtgccgcac ggtgagctga gcgagtttca gcttgagtgg
     8821 ctggaacgta aactggccga tgcgccagaa cgccatacgt tgctgctgct gcatcatcat
     8881 ccgctacctg cgggttgtag ttggctcgat caacacagtc tgcgtaacgc gggcgaactg
     8941 gataccgtgc tggcgaagtt tccgcacgtc aaatacttgc tgtgcggtca tattcatcag
     9001 gagctggatc tcgactggaa cggtcgccgc ctgctggcaa cgccgtcgac ctgtgtgcag
     9061 tttaagccgc actgttccaa ctttacgctg gatactatcg cgcccggctg gcgtactctc
     9121 gagttacatg ctgatggcac gctgaccacc gaggtgcatc gcctggcgga cacacgtttc
     9181 caacctgata ccgcttcaga aggctactga tgtctacgct tctttattta cacggtttca
     9241 acagctcgcc gcgctctgca aaagcgagct tgttaaaaaa ctggctggcg gaacatcacc
     9301 ctgacgttga gatgatcatt ccgcagttgc cgccgtatcc ttccgacgcg gcagaactgc
     9361 tggaatccat tgtcctggaa catggcggcg attcgctggg tattgtcggt tcgtcactgg
     9421 ggggatatta cgccacctgg ttgtcacaat gttttatgct gcccgcagtg gtggtaaacc
     9481 cggcggtgcg cccgtttgaa ctgctgacgg actatctcgg tcagaacgag aacccataca
     9541 ccgggcagca atatgtgcta gagtcacgcc atatttacga tctcaaagtc atgcagattg
     9601 acccgctgga agcgccggat ttgatctggc tgctgcaaca gacgggagat gaagtgctgg
     9661 attaccgcca ggcggtggcg tactacgctt cctgccgcca gactgtcata gaaggcggca
     9721 accacgcatt cacgggcttc gaagattatt tcaacccgat cgtcgatttt cttggtctgc
     9781 accatctctg acgatcaact cgaattgcta acttaaacca tgacgcaaac ttataacgct
     9841 gatgccattg aggtactcac cgggcttgag ccggttcgcc gccgtccggg gatgtatacc
     9901 gataccactc gccctaacca tttggggcaa gaagttattg ataacagtgt cgatgaagca
     9961 ctggcgggtc acgcaaaacg cgtggatgta atcttacatg ctgaccagtc gttagaagtt
    10021 attgacgatg ggcgcgggat gccggtggat attcacccgg aagagggtgt cccggcggtt
    10081 gaattgattc tttgccgtct gcatgcaggc ggtaaattct ctaacaaaaa ttaccagttc
    10141 tctggcggcc tgcatggcgt ggggatttcg gtggttaacg ccctgtcgaa gcgcgttgaa
    10201 gttaacgtgc gccgcgatgg tcaggtttat aacatcgcct ttgaaaatgg cgaaaaggtg
    10261 caggatttac aggttgtcgg cacttgcggt aaacgcaata ccggtaccag cgtgcacttc
    10321 tggccggatg aaaccttctt tgacagcccg cgattttctg tttcacgcct gacgcatgtg
    10381 ctgaaagcca aagcggtact gtgccccggc gttgagatta cctttaaaga tgagatcaac
    10441 aacaccgaac agcgctggtg ctatcaggac ggtctgaatg attacctggc ggaagcggta
    10501 aacggtttac cgacgctgcc agaaaaaccg tttatcggta atttcgctgg cgatactgaa
    10561 gctgtggact gggcgctact gtggctgccg gaaggcggtg aactgctgac cgaaagctac
    10621 gtcaacctga tcccaacgat gcagggcggt acccatgtta atggcctgcg tcagggcctg
    10681 ttggacgcga tgcgtgagtt ctgtgaatat cgcaacattc tgccgcgcgg cgtgaagctg
    10741 tcggcggaag atatctggga tcgctgcgcc tatgttctgt cggtgaaaat gcaggatccg
    10801 cagtttgccg ggcagacgaa agagcgtctc tcttcgcgtc aatgcgcggc attcgtttct
    10861 ggcgtggtga aagatgcctt tatcctgtgg ctgaaccaga acgttcaggc ggcggagttg
    10921 ctggcagaga tggcgatttc cagcgcccag cgtcgtatgc gtgcggctaa aaaagtggtg
    10981 cgtaaaaagc tgaccagcgg cccggcgctg cctggcaaac tggctgattg taccgcgcag
    11041 gaccttaacc gtaccgaact gttcctcgtg gaaggtgact ccgcaggcgg atctgccaag
    11101 caggcgcgcg atcgtgaata tcaggcgatc atgccgctga aaggtaagat ccttaacacc
    11161 tgggaagttt cttccgacga agtgctggcc tcgcaggaag tgcatgatat ttcggtagcg
    11221 atcggtatcg atcctgacag cgacgatctg agccagcttc gttatggcaa aatctgtatc
    11281 ctcgcggatg cggactctga tggtctgcac attgccacgc tgctctgcgc tttgttcgta
    11341 aaacacttcc gcgcgttggt gaaacacggt cacgtttacg tcgcactgcc accgctctac
    11401 cgtattgatc tcgggaaaga ggtttattac gcgctgacgg aagaagagaa agagggcgta
    11461 cttgagcaat taaaacgcaa gaaaggcaag ccgaacgtcc agcgttttaa aggtctgggg
    11521 gaaatgaacc cgatgcaatt gcgtgaaacc acgcttgatc cgaacactcg ccgtctggtg
    11581 cagttgacta tcgatgatga agacgatcag cgtactgacg cgatgatgga tatgctgctg
    11641 gcgaagaaac gctcggaaga tcgccgcaac tggttgcaag agaaaggcga catggcggag
    11701 attgaggttt aaaggaaaga acattgcccg gtcaggatgg ccgggcaaac aaggcaggat
    11761 taaatccctg gctgcagaat acgaatattc atctccagca cgtcgccttt tacggcttcg
    11821 ctgtacgcct tcatgtgcgg ggtttgcaga tgcgcttcaa ggtgcgcgat gctttcccac
    11881 tgctcaatca tcacgataga atccggtgcc atagactgga aactcacgcc agcagcgcaa
    11941 tccaccattg gcgcatagcc gtggcaacct tcttctttca gtacggttgg aacgatttta
    12001 gcaaactgat ccaataccgc ctgacggtga tgttgaccag gacgagtacg gatttctgcg
    12061 attacggtaa gcatggttaa ctccttctaa agcctgagct ctagttaacc aaaaatttcc
    12121 acaagatgct tgcgatattc ttcagtatag cggggaacat caggcatttt tatcacgtca
    12181 ttagcgataa atgtcggcag cggttccatg ccaaggaatt ggtttgcttt atggaacggc
    12241 agatacacac cgtcgacgcc aacgccgtgg aagaattgat ctttttcggt gaaggcttcc
    12301 attggtgcgt tccaggtcag agaaagcata tattttttgc cctgtaccag gccgccggaa
    12361 ccgtattttt ttgacggatc tttgcgggta cggccatcgc tggcatacag cgtcccgtga
    12421 ccttcggtga atacatcatc aatgtatttt ttcactgtcc acggcgcgcc catccaccag
    12481 cctggcatct gccagatcac cacatcagcc cagagaaagt tttgtacttc cgctttgaca
    12541 tcgtagtcgc tgtcggcgcg aacgatgcga acatcatgcc cgaggtcgcg cagtgtgcca
    12601 tccgcgactt cggtcagggt gtcgttcagt tgaccattgg agtgggcgaa ttttttcgcg
    12661 ccgttgataa tcagggtgtt gctcattttt taacctcaag gtgagacgtt tgtcgactat
    12721 agtacgtcac agagcggtac ggtaaaatta gcaaaatgtg caaagtcttt tgcgaattta
    12781 gcaaaagtct tacccttacc agctcacctt cgcctcaaat ccaccttgtt ccgcattccc
    12841 aaattcaaca ttcatgccat gcaatttggc gattcgctgg acaatcgata gcccaagtcc
    12901 actgccggta gcggtttgtc cgggggggcg atagaagcgt tcgccaattc gcgccagtgc
    12961 ctctggtgtc acaccggggc cgttatccct cacgatgaaa ttatcggcat tcagcgtgac
    13021 gtccaccacg ctgccctgtg gactgtagcg cacggcgtta tccagaaggt ttcgcaccaa
    13081 caaacttagc aatagcggtt gtccagtgcg tttgatgctg tgggcattga gtgtcagtcg
    13141 cacgtcaatt ttcgcctgct gcgccgtgtg gtaaatatcc atcaccgacg attgcaggag
    13201 atcttcaagc gggatctccg cgacgtcctg aaggttatcc agtgagtcca gccgcgatag
    13261 cgtgagcagt tgatcaacca gacgagtagc gcgatcgatc ccggaatgta attggagcag
    13321 tgcttttttc cgcgcctgcg gatcatcgtc agagagctgc gcaacttcgg tttgcacttt
    13381 cagcgctgtt aacgggctac gtagttcgtg agctgcatcg gaggtaaagc gtcgttctcg
    13441 aaccatcatc gcatgtgtgc gggcgaacag ttgatttagc gactcaacca gtggtcgcac
    13501 ttcgctgggt acgccagtcg cgtttagtgg tttttccgag tcagggtcac gcatacgtag
    13561 tgccagcgcc agtttgttca gcggcgcgag ttcacgaccc agtagtacca tcatgatgat
    13621 taacataatc ggcagtgcga ccagccacgg gatcaattgc ccggcaacaa tcgccagcgc
    13681 catgtcttca cggtattccc attcctggcc aacaacgatg cgatatttgc catcaggtga
    13741 ggtcatccag acaaaacgcc aaggatcgtc ttcaccgacc agttgcccgt cagcaaaacc
    13801 ttcccgttga tagctatagg gaatatcttc tccgttatcg ccatcattaa ggaccattct
    13861 gccgtcgtgg gtaaagatgg caaaggtcag cgcatcgtca tcaacatgac cgtgttttaa
    13921 tttatttggc gtctgtgcca tgcgatccgc cgcgttgatt tcgttgagat cgagcgtact
    13981 taaccgcttg gcaaacagca tcagttgggt gtcgaacaat tcatcgacgt tatccgttgt
    14041 ttgtttccag gcgacaaagc tggaaagcag ccaggtcacc gaggccagaa ttaaaaagat
    14101 tagcgtcagc ctgacgcgca gactaagacg ttgggtaaat ttcatttctc acctaatgtg
    14161 tagccaatac catgcacggt acgaataaaa tcactaccga gtttgcgtcg cagatgatgc
    14221 acatgcactt caacggcatt actggtgacc tcttcgtccc aggtatacag tttctcttca
    14281 atcagtttgc gcggcagtac ccgaccagcg ttacgcatca gtaattccag cagggcaaat
    14341 tcttttggtt tcagtgttaa gggttcgcca gccagcgtgg cgatacgttt gccggggtcg
    14401 agcatgacgt taccgtggcg cagctcgttg ctggcctggc cgttggttcg gcgcatcaga
    14461 gcctccagcc tggcggcgac ttctatcaac gcaaaaggtt tacacagata atcgtcagct
    14521 cccagacgca gcccttctac acgttccgct aacgcatcac gcgcggtcag gatcagtacc
    14581 ggctcacgct gacctttctc tcgccattcg cgcaaaatat cgcgaccatc catgcctggt
    14641 aaggttaaat ccaggatcac cgcatcataa ggtgcgctat aaagcgcctc ttttccctga
    14701 cgaccttgtg taaaccagtc gacactaaaa cccattttac taaggccagt tttgatgccg
    14761 tcgccaatca gcatgtcatc ttctatcagt aaaattcgca ttttttcatc cctgcgataa
    14821 ccgtatttgc tgccagtaaa ccgtgttgta acgcgctctg tacagcatta aattttattt
    14881 ttttccctta agaagttgtt aaggactatc ttgttaaatg ctcggaaaca gacattaaag
    14941 ggagtaataa acatgaaaaa attcgcagca gtaatcgcag taatggccct gtgcagtgca
    15001 ccggtgatgg cagcagagca gggcggtttt tctggcccat cggcaacgca aagtcaggcc
    15061 ggaggattcc aggggccgaa cggcagcgta acgactgtag aaagcgcaaa atccctgcgt
    15121 gacgacacct gggtaaccct gcgcggcaat atcgttgaac gcatctctga cgatctctac
    15181 gtgtttaaag atgccagcgg tactatcaat gttgatatcg accacaaacg ctggaacggt
    15241 gtgacggtga cgccgaaaga tacggttgag attcagggtg aagtcgataa agactggaat
    15301 tctgttgaaa ttgacgtcaa acagatccgc aaagtaaatc cgtaattgtt accgctcccg
    15361 ggacgcgttc ccgggaataa tttcgcaggg aggcaaaatg acaaacctga cactggatgt
    15421 aaacattatc gatttcccat caatccctgt ggcgatgttg ccgcaccgct gtagccctga
    15481 gttgctcaac tacagtgtgg cgaaatttat catgtggcgt aaagagacgg ggctttctcc
    15541 tgttaaccaa agccagactt ttggcgtcgc ctgggacgac cctgccacca ccgcaccgga
    15601 agcgtttcgc tttgatatct gcggcagcgt tagcgaaccg attcccgata atcgttatgg
    15661 tgtgagcaat ggtgaactta ccggtggacg ttatgccgtg gcccgccacg ttggcgagct
    15721 ggacgatatt tcacacacga tatggggcat tattcgccac tggctgcctg caagcggcga
    15781 gaaaatgcgt aaagcaccga ttctgtttca ctacaccaat cttgccgaag gggtgacgga
    15841 gcagcgactg gaaacggatg tttatgtgcc gttagcgtga tggggaatct ttaccggagt
    15901 gtgagccaaa tcactcgcga taagtttgag cgaacaatgc cacaggcgta ttgttgttgg
    15961 gcttataatt tctccggtaa ggaataccat gtatacgcga aatttattat ccggtgcgcc
    16021 gtaagccgtc tcgggggtac ccatgagcaa gccgcactta tggcaggagg cgcggggcac
    16081 tggtggagac acagctaaac tgggctgaac catatactta cagtcgacta ctttgatgct
    16141 ttaggcttcc ctggactcgc ataacctcaa cttctcgaat cgcccggagc ggacccccat
    16201 gccgagtggt gtggcatggg cgcaaccttg tgctgccctc tatgccgatc aagtctacag
    16261 caaagagttg tatatcaagg cacattgatg cctttatcgc cgtcggagca cgtccggcgg
    16321 cgctattcac gctgatctcc tgtgactcga cgcggcagat aatgtagtat ctccggcaat
    16381 attgcccctt tgaaggctgg cgaatcagtt gaggaatcag aattaatgag cgatatggca
    16441 gagcgccttg cgctacatga atttacggaa aacgcctact taaactactc catgtacgtg
    16501 atcatggacc gtgcgttgcc gtttattggt gatggtctga aacctgttca gcgccgcatt
    16561 gtgtatgcga tgtctgaact gggcctgaat gccagcgcca aatttaaaaa atcggcccgt
    16621 accgtcggtg acgtactggg taaataccat ccgcacggcg atagcgcctg ttatgaagcg
    16681 atggtcctga tggcgcagcc gttctcttac cgttatccgc tggttgatgg tcaggggaac
    16741 tggggcgcgc cggacgatcc gaaatcgttc gcggcaatgc gttacaccga atcccggttg
    16801 tcgaaatatt ccgagctgct attgagcgag ctggggcagg ggacggctga ctgggtgcca
    16861 aacttcgacg gcactttgca ggagccgaaa atgctgcctg ctcgtctgcc aaacattttg
    16921 cttaacggca ccaccggtat tgccgtcggc atggcgaccg atattccacc gcataacctg
    16981 cgtgaagtag ctcaggcggc aatcgcatta atcgaccagc cgaaaaccac gctcgatcag
    17041 ctgctggata tcgtgcaggg gccggattat ccgactgaag cggaaattat cacttcgcgc
    17101 gccgagatcc gtaaaatcta cgagaacgga cgtggttcag tgcgtatgcg cgcggtgtgg
    17161 aagaaagaag atggcgcggt ggttatcagc gcattaccgc atcaggtttc aggtgcgcgt
    17221 gtactggagc aaattgctgc gcaaatgcgc aacaaaaagc tgccgatggt tgacgatctg
    17281 cgcgatgaat ctgaccacga gaacccgacc cgtctggtga ttgtgccgcg ttccaaccgc
    17341 gtggatatgg atcaggtgat gaaccacctc ttcgctacca ccgatctgga aaagagctat
    17401 cgcattaacc tcaatatgat cggtctggat ggtcgtccgg cggtgaaaaa cctgctggaa
    17461 atcctctccg aatggctggt gttccgtcgc gataccgtgc gccgccgact gaactatcgt
    17521 ctggagaaag tcctcaagcg cctgcatatc ctggaaggtt tgctggtggc gtttctcaac
    17581 atcgacgaag tgattgagat cattcgtaac gaagatgaac cgaaaccggc gctgatgtcg
    17641 cggtttggcc ttacggaaac ccaggcggaa gcgatcctcg aactgaaact gcgtcatctt
    17701 gccaaactgg aagagatgaa gattcgcggt gagcagagtg agctggaaaa agagcgcgac
    17761 cagttgcagg gcattttggc ttccgagcgt aaaatgaata acctgctgaa gaaagaactg
    17821 caggcagacg cgcaagccta cggtgacgat cgtcgttcgc cgttgcagga acgcgaagaa
    17881 gcgaaagcga tgagcgagca cgacatgctg ccgtctgaac ctgtcaccat tgtgctgtcg
    17941 cagatgggct gggtacgcag cgctaaaggc catgatatcg acgcgccggg cctgaattat
    18001 aaagcgggtg atagcttcaa agcggcggtg aaaggtaaga gcaaccaatc ggtagtgttt
    18061 gttgattcca ccggtcgtag ctatgccatc gacccgatta cgctgccgtc ggcgcgtggt
    18121 cagggcgaac cactcaccgg caaattaacg ttgccgcctg gggcgaccgt tgaccatatg
    18181 ctgatggaaa gcgacgatca gaaactgctg atggcttccg atgcgggtta cggtttcgtc
    18241 tgcaccttta acgatctggt ggcgcgtaac cgtgcaggta aggctttgat caccttaccg
    18301 gaaaatgccc atgttatgcc gccggtggtg attgaagatg cttccgatat gctgctggca
    18361 atcactcagg caggccgtat gttgatgttc ccggtaagcg atctgccgca gctgtcgaag
    18421 ggcaaaggca acaagattat caacattcca tcggcagaag ccgcgcgtgg agaagatggt
    18481 ctggcgcaat tgtacgttct gccgccgcaa agcacgctga ccattcatgt tgggaaacgc
    18541 aaaattaaac tgcgcccgga agagttacag aaagtcactg gtgaacgtgg acgccgcggt
    18601 acgttgatgc gcggtttgca gcgtatcgat cgtgttgaga tcgactctcc tcgccgtgcc
    18661 agcaacggtg atagcgaaga gtaatatgcc ggggagggcc tgtttcctcc cgcttgcaag
    18721 gaacgccgga tgaaacgtta agtcgtcatc cggcagcgta ttcaaccccc ggaaacgata
    18781 aaaattccct tgaaaccgtt gtttattcat gcgttgcgat taacaatacg cttttccaga
    18841 gagcggcttt taacaatgcc ctaacctgat ttcaggtgac gtacaatgcc agtctctcag
    18901 accttcagag ggtgttatgc tatatatctt tcgtcttatt attaccgtga tttacagcat
    18961 cttagtctgt gtattcggct ccatttactg ccttttcagc ccgcgtaacc cgaaacatgt
    19021 ggccaccttt gggcatatgt ttggccgtct tgcgccgctg ttcggcctga aagttgagtg
    19081 ccgcaaacct gcagatgctg aaagctacgg caatgctatc tatatcgcta accaccagaa
    19141 caactatgac atggtgacag catcgaacat tgtgcaaccg ccgacggtga cggtaggtaa
    19201 aaagagcttg ctgtggatcc ccttcttcgg gcagttgtac tggttaaccg gcaacttatt
    19261 gatcgacaga aacaatcgca ctaaagctca cggcaccatt gcggaagtcg tgaatcattt
    19321 caaaaaacgc cgtatttcca tctggatgtt cccggaagga acccgcagcc gtggtcgcgg
    19381 cctgctaccg ttcaagactg gagcatttca cgcggcaatt gcggcgggcg tcccgattat
    19441 tcccgtgtgc gtctctacaa cttcgaataa gattaatctt aatcgactgc acaacggtct
    19501 ggtgattgtc gaaatgctgc cgccaattga cgtcagtcag tatggcaaag atcaggttcg
    19561 tgagctggct gcccattgtc gttcgataat ggaacaaaaa atcgccgagc tcgataaaga
    19621 agtcgcagaa cgcgaagccg ccggaaaagt ttaagtcggc aatctgtatt tttgcgggga
    19681 acactttcct gcacggtatt actttagcca gttttacatg gagcaaatat gtcactcagt
    19741 cggcgtcagt tcattcaggc atcggggatt gcactttgtg caggcgctgt tcccctgaag
    19801 gccagcgcag ccgggcaaca gcaaccgcta cccgttccgc cgctacttga atctcgccgt
    19861 gggcaaccgc tgtttatgac tgtacaacgt gcgcactggt catttacgcc agggacacgc
    19921 gcgtcggtct ggggaatcaa tggtcgttac ctggggccga ctatccgcgt ctggaagggc
    19981 gacgatgtta agcttattta cagcaaccgc ctgacagaaa atgtctcaat gacggtggcc
    20041 gggctacagg tacctggccc gctgatgggc ggtccggcac ggatgatgtc gccaaacgct
    20101 gactgggcac ccgtactgcc tattcgccag aacgcagcta ctctgtggta tcacgccaat
    20161 actcccaacc gcacggctca gcaggtctat aacggccttg ccggaatgtg gctggtggaa
    20221 gatgaagtca gcaagtcgct gcctatcccc aaccattatg atgtggatga ttttccggtc
    20281 attatccagg ataaacggct ggataacttt ggtacgccag aatacaacga accgggaagc
    20341 ggcggctttg ttggtgatac gctgctggtt aacggtgtac aaagcccgta cgttgaagtc
    20401 tcgcgtggct gggtgcgctt gcgactgctg aacgcgtcga actctcgtcg ctatcaacta
    20461 cagatgagcg atggtcgccc gttacatgtg atttctggcg atcagggatt cctgcctgct
    20521 cctgtatcgg tgaagcaact ttcgctggca ccgggcgagc gccgcgagat tctggtggat
    20581 atgagcaacg gcgatgaagt gtcgatcacc tgtggcgaag cggcgagcat tgttgatcgt
    20641 attcgcggct tctttgagcc atccagtatt ctggtttcta ccctggtgct aacgctgcgc
    20701 ccaaccggcc ttctgccgct ggtcacagac agtcttccga tgcgcttgct gccaactgaa
    20761 atcatggccg gttcgccgat tcgcagtcgc gatatcagtc tgggtgatga cccgggtatt
    20821 aatggacagc tgtgggacgt caaccgtatt gatgtcaccg cgcagcaagg aacgtgggaa
    20881 cgctggacgg tacgcgcgga cgagccgcaa gcgttccata ttgaaggcgt gatgttccag
    20941 atccgtaacg tgaatggtgc gatgccgttc ccggaagaca gaggctggaa agataccgtt
    21001 tgggttgacg gacaagtgga gctgcttgtt tatttcggtc agccttcctg ggcgcacttc
    21061 ccgttctact tcaacagtca gacgctggaa atggcggacc gtggctcgat tgggcaactg
    21121 ttagtcaatc cggtaccgta atcttcatac tcgcccgagg agggcgagta tggaatactt
    21181 ttcgatttct ctttcgttcc ggcttataat ccccggcctt tgattatttt tttatcattt
    21241 ctggaagcaa aatgagctct atctccctga tccaaccgga tcgcgacctg ttctcctggc
    21301 cgcagtactg ggccgcctgt tttggaccgg caccgttttt gccgatgtct cgtgaagaga
    21361 tggatcaact tggctgggat agctgcgaca tcattttggt tactggcgac gcgtatgtcg
    21421 atcacccaag cttcgggatg gcgatttgcg gtcgtatgct ggaagcacag ggctttcgcg
    21481 tcgggatcat cgcccagccg gactggagca gcaaagacga ctttatgcgt ctgggtaaac
    21541 cgaatctgtt tttcggtgtt actgctggca acatggattc gatgatcaac cgttataccg
    21601 ccgatcgccg tttacgtcat gacgatgcct acacgccgga taacgtcgcg ggtaagcgcc
    21661 cggatcgcgc cacactggtt tatacccagc gttgtaaaga ggcgtggaaa gatgtaccgg
    21721 tgatcctcgg cggtattgag gctagtctgc gccgtaccgc acattatgat tactggtccg
    21781 ataccgtgcg ccgttccgtg ctggtggatt cgaaagccga catgctgatg tttggtaacg
    21841 gtgagcgtcc gctggtggag gtggcgcacc gtctggcgat gggcgagccg attagtgaaa
    21901 tccgcgatgt gcgtaatacc gcgattatcg tgaaagaggc gctgcctggc tggagcggcg
    21961 tggattccac ccgtcttgat acccctggaa aaatcgaccc aatcccgcat ccgtatggtg
    22021 aagatttgcc gtgcgcggat aacaaaccgg tggcaccgaa aaagcaggaa gccaaagccg
    22081 taaccgtgca gccaccgcgc ccgaaaccgt gggaaaaaac ctacgtgttg ctgccttctt
    22141 tcgagaaagt gaagggcgat aaagtgctgt acgcccatgc ttcgcgtatt ctgcaccacg
    22201 aaaccaaccc aggctgcgtc cgcgcattga tgcaaaaaca cggtgaccgc tatgtgtgga
    22261 tcaacccgcc tgctatcccg ctttctaccg aagagatgga cagcgttttt gcgctgccgt
    22321 acaagcgcgt gccacatccg gcctatggca atgcccgtat tccggcttac gaaatgatcc
    22381 gtttttcggt caacattatg cgcggctgct ttggcggctg ttctttctgt tctattaccg
    22441 agcacgaagg gcgcattatt cagagccgtt cggaagattc gatcattaat gagatcgaag
    22501 cgatccgcga caccgttcca ggttttacgg gcgtgatttc cgatctcggt gggccaactg
    22561 ccaacatgta tatgttgcgc tgcaaatcgc cacgcgctga acaaacttgt cgccgtttgt
    22621 cgtgcgttta tccggatatt tgtccgcaca tggacactaa ccacgaaccg acgatcaacc
    22681 tctatcgccg tgcgcgtgat ctgaaaggca ttaaaaaaat cctcatcgcc tcaggcgtac
    22741 gttatgacat cgccgtggaa gatccgcgct atatcaaaga gttggcgacc catcacgtcg
    22801 gcggttatct gaagattgct ccggaacata ccgaagaagg gccgttgtcg aagatgatga
    22861 agccgggcat gggcagctat gaccgcttta aagagctgtt cgatacctac tcgaaacagg
    22921 caggtaaaga gcagtatctg atcccgtatt tcatctctgc gcaccccggt acgcgtgatg
    22981 aagatatggt gaatttggcg ctgtggctga aaaagcatcg tttccgtctc gaccaggtac
    23041 agaacttcta cccatcgccg ctggcgaact caaccaccat gtattacacc gggaaaaaac
    23101 cgctggcgaa gattggttat aagagcgaag acgtcttcgt accgaagggc gacaaacagc
    23161 gtcgtttgca taaagcgttg ttgcgttacc acgatccggc aaactggccg ttaatccgcc
    23221 cggcgctgga agcgatgggc aaaaagcatc tgattggcag ccgtcgcgat tgcttagtgc
    23281 ctgcgccaac cattgaagag atgcgtgaag ctcgtcgcca gaaccgcaat acccgtccgg
    23341 cgttgactaa acatacgccg atggcgaccc agcgtcagac gcctgctacg gcaaaaaaag
    23401 cgtcgtctac gcaatctcgt ccggtgaatg ctggtgcgaa gaaacgccct aaagcggcgg
    23461 ttggacgtta aaaaaaatcc ccggcaactg acatgctgcc ggggattttt ttatcattct
    23521 gcgtacatac gcaagcagcg caggcaatca aaatagtcct gagcatcaag acgcgtattg
    23581 gctctgttag cttcggattt ggcagcatag atttgtgggc gtagtgcttt tatatcctgc
    23641 tcaagccggg cgattttggc atttaatgtt tgtacattgc tcgccagttg attgaccttt
    23701 tgctcattga cgcagccact gagcaagcag gttgccagaa caacagctcc cacggtgaaa
    23761 atcgttttca tatagtgttc ccttttattt attgttccgg ctaattgtta tttaattcag
    23821 ctcattgctt taatctggcg gcggtttttg cctcaatctg cttaagtagg ctgccgctgt
    23881 tcgggcggat aaaatcagcg atttcccact tgccttcgcg ccgctgtaaa accagttctt
    23941 gctcttcgtt gttgccattg gtcagcgtaa aacgaacatg aatgcgggcg gaatcggcgg
    24001 ttttctccag gactcgaatt tcatcaacgc gtgaaaatag cgactcttcg ccaaaacaga
    24061 tcggattgtc gtaaataaat tgccgttcta tttgcatatc ttcatcggga ttattggccg
    24121 tcttgaaggt ggttttatag aaggcattaa ccgcctggcg gtaagagtcg gaaaccaccc
    24181 acgtccacgg ccagctataa tcagcaatat gttcaaagag ttcggcaaca aaggcttccg
    24241 gctgttcttt ttgcaacgaa gctaaagcgg ccagcgtttt ttcattctcg ctattaactg
    24301 cttgtaagac gctgccatga ttgctgacaa tatcgtcaat gacccaacga ccattttccg
    24361 ccaccatttt cagtgtctgc gtggtctttt ctttatcatc tttaaagata cgaaagcgca
    24421 caacggcatc ggcatgatcg gcgtcagttt gggttatcgc aacgctttct agcaccagat
    24481 cgccaaaatc ctgacaatca caaaccggat cataatccag ccagccaata ttgcccggca
    24541 gcgtaagatt gtcgttcagg gtaagcgcct gttgaatacg cgctgaagtt atcgcccgct
    24601 ctccggtttc accgaaataa ggtgtactgg catctgattt atagttctga taaatctgcc
    24661 ggactgtttg ttctacggtc tgtgaaggag gctgcgcgtg tacggtaaaa cttgccaggg
    24721 ctgctaaaaa cagaagtatt attttcatct gtttttcacc tctccgttgc gtttatcccg
    24781 gatatagatg agttgttctt ttaagagctt ccggct
//
LOCUS       DAZPDU010000050        24370 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZPDU010000050 DAZPDU010000000
VERSION     DAZPDU010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24370
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.050"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(140..1021)
                     /gene="prmA"
                     /locus_tag="ACHURS_001836"
     CDS             complement(140..1021)
                     /gene="prmA"
                     /locus_tag="ACHURS_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFR1237588.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     IVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYVDSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(1033..2484)
                     /gene="panF"
                     /locus_tag="ACHURS_001837"
     CDS             complement(1033..2484)
                     /gene="panF"
                     /locus_tag="ACHURS_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFR1237589.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(2474..2716)
                     /locus_tag="ACHURS_001838"
     CDS             complement(2474..2716)
                     /locus_tag="ACHURS_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFR1237590.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(2825..4174)
                     /gene="accC"
                     /locus_tag="ACHURS_001839"
     CDS             complement(2825..4174)
                     /gene="accC"
                     /locus_tag="ACHURS_001839"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFR1237591.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(4185..4655)
                     /gene="accB"
                     /locus_tag="ACHURS_001840"
     CDS             complement(4185..4655)
                     /gene="accB"
                     /locus_tag="ACHURS_001840"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFR1237592.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(5633..6607)
                     /locus_tag="ACHURS_001841"
     CDS             complement(5633..6607)
                     /locus_tag="ACHURS_001841"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFR1237593.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            6759..8699
                     /gene="csrD"
                     /locus_tag="ACHURS_001842"
     CDS             6759..8699
                     /gene="csrD"
                     /locus_tag="ACHURS_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFR1237594.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            9004..10047
                     /gene="mreB"
                     /locus_tag="ACHURS_001843"
     CDS             9004..10047
                     /gene="mreB"
                     /locus_tag="ACHURS_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFR1237595.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            10113..11216
                     /gene="mreC"
                     /locus_tag="ACHURS_001844"
     CDS             10113..11216
                     /gene="mreC"
                     /locus_tag="ACHURS_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFR1237596.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            11216..11704
                     /gene="mreD"
                     /locus_tag="ACHURS_001845"
     CDS             11216..11704
                     /gene="mreD"
                     /locus_tag="ACHURS_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFR1237597.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            11713..12306
                     /gene="yhdE"
                     /locus_tag="ACHURS_001846"
     CDS             11713..12306
                     /gene="yhdE"
                     /locus_tag="ACHURS_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFR1237598.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            12296..13765
                     /gene="rng"
                     /locus_tag="ACHURS_001847"
     CDS             12296..13765
                     /gene="rng"
                     /locus_tag="ACHURS_001847"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFR1237599.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            13833..17633
                     /gene="yhdP"
                     /locus_tag="ACHURS_001848"
     CDS             13833..17633
                     /gene="yhdP"
                     /locus_tag="ACHURS_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFR1237600.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWHYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQYITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            17789..19234
                     /gene="tldD"
                     /locus_tag="ACHURS_001849"
     CDS             17789..19234
                     /gene="tldD"
                     /locus_tag="ACHURS_001849"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFR1237601.1"
                     /translation="MSLNLVSEQLLAANSLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(19362..20291)
                     /gene="aaeR"
                     /locus_tag="ACHURS_001850"
     CDS             complement(19362..20291)
                     /gene="aaeR"
                     /locus_tag="ACHURS_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFR1237602.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            20474..20677
                     /gene="aaeX"
                     /locus_tag="ACHURS_001851"
     CDS             20474..20677
                     /gene="aaeX"
                     /locus_tag="ACHURS_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFR1237603.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            20685..21617
                     /gene="aaeA"
                     /locus_tag="ACHURS_001852"
     CDS             20685..21617
                     /gene="aaeA"
                     /locus_tag="ACHURS_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFR1237604.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGGRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            21623..23590
                     /gene="aaeB"
                     /locus_tag="ACHURS_001853"
     CDS             21623..23590
                     /gene="aaeB"
                     /locus_tag="ACHURS_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFR1237605.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVFAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            23682..23954
                     /locus_tag="ACHURS_001854"
     CDS             23682..23954
                     /locus_tag="ACHURS_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFR1237606.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(24010..24273)
                     /gene="yhcN"
                     /locus_tag="ACHURS_001855"
     CDS             complement(24010..24273)
                     /gene="yhcN"
                     /locus_tag="ACHURS_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFR1237607.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDTAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         5779 a         6159 c         6594 g         5838 t
ORIGIN      
        1 gaaatttgag taagttctca atttttcttt atgaatgaaa attttggcac gcaaatttgc
       61 gtaaataacc ggcaaattta cgtgcctttt gtgagcttgc tcgcacttcg ccccgcgtca
      121 ccctacggcg atgcgaaggt taattcttac gaccggtaat gcggcaccac tcttcttttt
      181 ccacgaccgg gtccagtgcg aagctgtcga cataagcttc acaaacgctc tctgcctggc
      241 ttgccagaat accggaaagg cccagcaaac cgcctgaaac cggcaggacg ctgattaacg
      301 gtgccagttc acgtaatggg cctgcaagga tgttagcgac caccacgtcg gctttcattt
      361 cttctggctg atctttcggt aagtagagtt ccagacggtc agaaacgcca ttacgttcgg
      421 cgttgtcgcg gctagcctga atcgcctgcg gatcgatatc aataccaatg gcttttgctg
      481 cacccagttt cagcgccgcg atagccagaa tgccggaacc acagccaaag tcgatgacta
      541 ttttaccggt taaatcgagg ctgtcgagcc actgcaggca cagagaggta gttggatggg
      601 tacccgtacc aaacgccagc cctggatcta acatcacgtt gacggcgttt tcgtccggca
      661 catcacgcca gctagggcag atccacagtc gttcaccaaa gcgcatcggg tggaaattat
      721 ccatccattc gcgctcccag tctttatctt ccagttgttc gattttatgc gcgaagcctg
      781 cgccgagcag cggatggttt tccagaatcg ccaccacgtc gttcatatcg gtttcagcgt
      841 cgaacagacc aatcacatcg gtgtcgcccc acaggcgcgt ttcgcccggc agcggctcaa
      901 atactggcgt atcgtgggta tcctgaaaag tgatagaaac ggcacccgct tccatcagcg
      961 catcgctaag atcttcagcg ttcgcgccgg tggtgttcag tttcagttgg atccaaggca
     1021 tggcaaaact ctttatttat cagtagtcaa aacggtagct tgcgggacgg atgtaccgaa
     1081 acggtttccg accaggaaag ccagcaaact tagtagtaac gagggcacga tagggtggaa
     1141 gcccaggtac tgaatattca gcgtcgcgag tacggcatac agcacgccgc caacgatcat
     1201 cgcacttagc gcgcctttgg cgttggcgcg ttcccagtaa agacccagca ccagcggcca
     1261 caggaaaacg gcttccagcc caccgaaggc cagcaaattc agccagatga tcatttctgg
     1321 cggcttccag gcggcaagca gcagcaacgc gccgagaact aacgtaatta ccgccgacat
     1381 ccgcttcaga cgcgtctcgt tttgcatttg atccggacgg atattcagat agagatcttt
     1441 aatgatcgta gcggaacttt gtagcaattg ggcgttaatt gtcgacatga tcgcagccat
     1501 aggtgcagcc agaaagatcc cggcagcaaa cggtggcagc acttttacca ttaacgtcgg
     1561 gatcaccaga tccggtacgg tgagatcggg gatcactgca cgacctaacg ctccggctag
     1621 gtgcataccg aacatcagga ttgcgactac aatcgtaccg atgatgatcc cccggtgtac
     1681 cgctttgctg tctttataag agatacagcg caccgcagta tgcggcaggc caatcacgcc
     1741 aaagcacacc agtacccaga acgacgtcat aaaggcaggc gacagaatat cgtcagcgcc
     1801 ttgtggcgta accagttgcg gatcgatggt ttgcaaggtc tgtactgcgt tgcttaagcc
     1861 gccagcggca tgtaccacgc caataagcag cacaacggtg ccaatcagca tcacaagccc
     1921 ttgcatggtg tcgttcagca cgctggcgcg aaagccgcca aaggcggtat acaacgcaat
     1981 gctgataccg aaaatcagca gcccggtttc ataaggaata cccgccgcgg tttccagcag
     2041 gcgcgcaccg ccgataaact gcacggtcat tgccccaacg aacgcaacca gcaaactcaa
     2101 actcgccagc cacaccagaa gacgactctg gtagcgggca aacagcatgt cgttcagcgt
     2161 cactgcattg tagcggcgcg caagaatcgc aaacttcttg ccgagaatac cgagtgaaag
     2221 ccagactgca ggaagctgaa tcatcgccag caatacccag cccagcccgt atttataagc
     2281 agctcctggc ccgccgataa acgaactggc actgatatag gtcgcggtga gcgtcatcgc
     2341 cagcacaata ccgcccatag agcggctgcc gaggaaatac tcattaagga aggtgccggt
     2401 gctccgttta cgcatcgcat aaaccgagat accgaacacc accaccagat aggcgaccag
     2461 cggtagaatt acttcaagct gcatcgtcat cctccagtgg gatatcgcga tagataaatt
     2521 tcaccatcgc ccagcacagt ccaataaaca gtagcggcgt caggatgcag gccatctcaa
     2581 accagcgcgg aaagccggta aaaccggggg caacgccagg taagtaagcg gctactaacc
     2641 aaactgccag atacaaaagg gtcagcccca gcgcccagcg cgcctcttta tgggcctgaa
     2701 caaaacgagt gtccattttt tgtccctgat gggtgaagaa agcgaggatt gtaccttatg
     2761 ggggttgtcg atccccagta ataaaaaagg ccggaaaatc cggccttttg acgcttttgc
     2821 agtcttattt ttcctgaaga ccgagttttt tctccagata gtggatgtta gtgccaccat
     2881 gctggaagtt ctcgtcattc atgatgcgga tctgcagatc aacgttggtt ttgataccgt
     2941 cgatgatcag ctcctgcagc gcattcttca tgcgggcaat cgccacgtca cggttttcac
     3001 cgtagcaaat cagcttaccg atcattgagt catagtacgg cggtacggtg tagcccgcgt
     3061 agatatgaga ctcccaacgt acgccaaaac cgccaggtgc gtggaaacgg gtgattttgc
     3121 ctgggcttgg caggaaggtg ttcggatctt cggcgttgat acgacattcc accgcatggc
     3181 cgcgaacgtg aacttcttct tgcttgatcg acagcggttg accggcagcg atacggagct
     3241 gttctttgat caggtcaacg ccagtgatca tttcggtaac cgggtgttct acctgaatac
     3301 gggtgttcat ttcgatgaaa tagaactcgc cgttttcgaa cagaaactcg aaagtacctg
     3361 caccgcgata gccgatatca acacacgctt tagcgcaacg ttcgccgatg tagcgacgca
     3421 gttccggggt aatgcccggt gctggcgctt cttcgaccac tttctggtgg cggcgctgca
     3481 tggagcagtc acgttccgcc agatagatag cgttgccctg accgtcagcc agtacctgaa
     3541 tctcgacgtg gcgaggattt tccaggtatt tctccatgta aaccatatcg ttgctgaaag
     3601 cagctttcgc ttccgcacgg gtcatggaga tggattgtgc cagttcagcg tcgccgcgca
     3661 ctacgcgcat accgcgacca ccgccgccgc cggaggcttt gataatcacc ggataaccaa
     3721 tgcgtttagc aatggcacgg tttttatcca tatcgtcgcc caacgggccg tcagaacccg
     3781 gtacgcaagg gacgcccgct tttttcatcg ccgcgattgc ggatactttg tcgcccatca
     3841 ggcgaatggt ttctgctttc gggccgatga agataaagcc ggagcgttca acctgctcgg
     3901 caaagttggc gttctcggag aggaagccgt aacccggatg gattgctact gcgccggtga
     3961 tttcagcggc gctgatgatt gccgggatgt tcagataact ttttactgac ggagcagggc
     4021 caatacagac cgtttcatct gccagtaata cgtgttttag atcgcgatcc gcgctggagt
     4081 gcacagcgac agtcttgatg cccagttctt tacaggcacg aagaatacgc aatgcaatct
     4141 cgccgcggtt ggcaataaca attttatcca gcatgttcgc ctcgttactc gatgacgacc
     4201 agcggctcgt caaattctac cggttgtcca ctttcgacca gaattgcttt cacggtaccg
     4261 gatttgtccg cttcgatctg gttcatcatt ttcatggctt caacgatgca cagggtatcg
     4321 cccacgttga ctttctgacc cacttcgatg aacgcttttg cgtccgggct tggggtgcgg
     4381 tagaaagtac caaccatcgg ggaacgtacg atgtgaccac tgatttccgc tgctgctggc
     4441 gcttccatgg aaggaacggt cgccggagcg gctgcgttag attgagctgg ctgctgcatc
     4501 attggtgcag cgtaagcttg ttgcatcaca gggaaacttg cggcaggagc tgcacggcta
     4561 atgcgtactg actcttcgcc ttcagaaatt tccagttcgg agatgcctga ttcttcaacc
     4621 agctcgatca gttttttaat cttacgaata tccatgagtg ggttccgtac tctttgttta
     4681 gtgtgattgt gacaggcgtt tcaccgccgt ctgtaaagcg tatgccccga cagctgtatg
     4741 catagcgata aattccagca ggccgggacg ccggtctatt ttgccccaga gtatcaatgt
     4801 tagacttgac ggacattgtg cagcgatctg ctgctatcct ccggcaaaaa acaaaatata
     4861 ccttcgttgc gccaatgtca ccttttccgc agcgcaaaaa ctgcgtcagg gaggacgagg
     4921 tcgcacatta taacgatttc gtagcaattg gcagctaaat actggtctca tcagggaaga
     4981 taatcaacag ctaacatgta aataaccttc aacaccgtgt aatttgcaac aagccgcaca
     5041 attcacgaaa ttagcgccat catcgacgga acttcttata acgtaaggtg aaaagctgta
     5101 aagccagccc tgcgaagatg aggggctgca atgagataat cttcacagac cacaaataat
     5161 gtagggatgc caggatcgcg acaagataga cgacattacg cagcggttgc tggggattac
     5221 ccaattttca ctgcatcgtc tggatagatg tgaatgctaa agcacgcaaa atcaaccgac
     5281 tgacaattct cagcattaaa taggttcgag tggttaattt gtagtcaaat aaaatcagat
     5341 tattcacccc atggtaccag aaatcagtaa caggttaagc gtagtgtcgc ccaggcaaag
     5401 caccataaac ctaacaggcg acggaggcgt atcaataatg gctgtttaga ggagtatcaa
     5461 tgcgcatcaa tttgtggtca gtcgaaatca tgactttcca gtttggatga tgttgtacaa
     5521 cttcctgacc aaggtgttcg acgaaagctg tccggctaac cgcttttccc gttaaccgat
     5581 tgaataatga tgtcaaaatg agttccctct cttattattc cttgctaaat ggttagttaa
     5641 ccttcaccag cgtgcgaccc tggatctggt tattaatgat ggcctcggca aagttcggtg
     5701 cctctgacag agatatctct tttgccgcct gggtatagaa tgattccggt aaatcggcga
     5761 ccagtcgctg ccaggcttgt gcgcggcgtt ctggtggcgt cattactgaa tccacccctt
     5821 gcaaacggac attacgcaga ataaatggca tgaccgtggt tggcagagta aaaccacccg
     5881 ccagaccaca ggccgccacg cagccgccgt aattcatttg cgccagcact tttgccagca
     5941 ctttgtcgcc aacagtgtca attgccccag cccagacttg tttttccaga ggacgggatt
     6001 cggcaaactc atcacgaggg agaatacggc tggcacctaa acttttcaga tattcatggg
     6061 tactttcgcg accggaaacg gcaacgacct gataacccaa cttatgcagc agcgccacgg
     6121 cggtactgcc gacgccacca ctggcacccg tcacgacaat ctccccgtcc tgcgggcgaa
     6181 caccggcatc ttccagcgcc atcacacaca gcatggcggt aaaaccggca gtaccgataa
     6241 tcattgcttt acgcgcgtcc agcccttgcg gcatggcaac cagccagtca cctttcactc
     6301 gcgcctgctc cgccagccca ccccagtggt tttcaccaac gccccagcca gtgagtaaca
     6361 cctcctgacc ggcatgaaaa cgcggatctt cgctggtgcg tacagttccg gcaaaatcga
     6421 tcccaggaat catcggaaaa ttacggatga tttttccctt accggtaatc gccagcgcat
     6481 ctttatagtt caggctcgac cagtgaacat cgaccgtgac atcgccctcc ggcaggcgac
     6541 tttcgtccag agtctgtact gatgcgagag ttttgccatc ctgctgttct aaaagtaacg
     6601 cctgcataag tggtcctcat gtgcatgatg gattggaaaa taatttctga agattatact
     6661 cgctaatgga aaagcaaagc cgatgaagcg caataaattg cgagataaat ctgatttgct
     6721 agtatgcccg cttcctcact atcggagtta acacaaggat gagattaacg acgaaatttt
     6781 cggcctttgt tacgctgctc accgggttaa caatttttgt gactttgctg ggctgttcgc
     6841 taagtttcta caacgcaatt cagtataagt ttagtcatcg cgttcaggcg gtggcgacgg
     6901 cgattgatac ccaccttgtg tcgaatgact tcagcacatt aaggccacaa attaccgaat
     6961 taatgatgtc ggcagatatc gttcgtgtag acctgctcca tggtgataaa caggtttata
     7021 ccctggccag aaatggtagt tatcgtccgg ttggctccag cgatctgttt cgcgaactga
     7081 gtgttccgtt gataaagcat ccggggatgt cgctgcgtct ggtttatcag gatccgatgg
     7141 gcaactattt ccattcttta atgactaccg cgccgctcac gggggcgatt ggctttatca
     7201 ttgttatgct cttcctggcg gtacgctggt tacaacggca acttgccggg caagaattgc
     7261 tggaaacccg ggctactcgt atcttaaacg gtgagcgtgg ctctaatgtg ttgggaacca
     7321 tctatgaatg gccgcccaga accagcagtg cgctagatac gctgcttcgt gaaattcaga
     7381 acgcacgcga acaacacagc cgtcttgata cgctgatccg ctcttatgcc gcccaggacg
     7441 tgaaaaccgg cctcaataac cgactctttt tcgataatca gttagcaacg ttactggaag
     7501 atcaggagaa agtaggtacc cacgggatcg tgatgatgat tcgtctgccg gatttcaata
     7561 tgttgagcga tacttggggg cacagccagg ttgaagaaca gttcttcact ctgactaatc
     7621 tgctgtcgac atttatgatg cgctaccctg gcgcactgct ggcgcgttac caccgcagtg
     7681 attttgctgc gctgttaccg caccggacgt taaaagaggc agagagcatc gccggtcagt
     7741 taatcaaagc cgtcgatacc ttgccgaaca ataaaatgct cgatcgcgac gatatgatcc
     7801 acattggtat ctgcgcctgg cgtagtggtc aggataccga gcaggtaatg gaacatgcag
     7861 agtctgccac gcgtaatgcg ggattgcagg gcggcaatag ctgggctatt tacgatgact
     7921 cgttgcctga aaaaggacgc ggtaatgttc gctggcgtac gcttatcgag caaatgctca
     7981 gtcgcggcgg cccgcgcctt tatcaaaaac cggcggttac tcgcgaaggt caggttcatc
     8041 atcgcgaact catgtgccgc atcttcgatg gtaatgaaga ggttagctcg gcggagtata
     8101 tgccgatggt cttgcagttt ggcttatcgg aagagtatga ccgtctgcaa atcagccgtc
     8161 ttattccact attgcgttac tggccagagg aaaatctggc gattcaggtt accgttgagt
     8221 cgctgattcg cccgcgtttt cagcgttggc tgcgcgatac gttaatgcaa tgtgaaaaat
     8281 cacaacgaaa acgcataatt attgaacttg cagaggccga tgtaggtcaa catatcagtc
     8341 gtttacaacc tgttattcgt ttagtgaatg ctttaggggt acgggtagcc gtcaaccagg
     8401 ctggtttgac gctggtaagc accagttgga tcaaagaact taatgttgag ttactcaagc
     8461 tccatccggg gctggtcaga aacattgaga agcgaacgga gaaccagctg ctggttcaaa
     8521 gcctggtgga agcctgctcc gggaccagca cccacgttta cgccaccggc gtgcgttcgc
     8581 gaagcgagtg gcagaccctg attcagcgcg gtgttacagg cgggcaaggg gattttttcg
     8641 cgtcctcaca gccacttgat actaacgtga aaaaatattc acaaagatac tcggtttaac
     8701 ctgccgttta atccgttttc acgtagaata acgcgcgctg cgtctcatgg gagtgtgctt
     8761 gtctgctcgc cagattgttg cagcacatat gcagatgaat gaccttacgc ggttgcaaac
     8821 aggcgaggaa tgctgctgat gcattaagcc tttctggact caggcagaga tttgtaacaa
     8881 aggaaacgaa ctgcactaat tttcaccgta gcagatgatt tttgcgcctt gtcgctgctg
     8941 cgtgtggttg gtaaagtaag cggattttct tttccgcccc agctttcagg attatccctt
     9001 agtatgttga aaaaatttcg tggcatgttt tccaatgact tgtccattga cctgggtact
     9061 gcgaataccc tcatttatgt aaaaggacaa ggcatcgtat tgaatgagcc ttccgtggtg
     9121 gccattcgtc aggatcgtgc cggttcaccg aaaagcgtag ctgcagtagg tcatgacgcg
     9181 aagcagatgc tgggccgtac gccgggcaat attgctgcca ttcgcccaat gaaagacggc
     9241 gttatcgccg acttcttcgt gactgaaaaa atgctccagc acttcatcaa acaagtgcac
     9301 agcaacagct ttatgcgtcc aagcccgcgc gttctggttt gtgtaccggt tggcgcgacc
     9361 caggttgaac gccgcgcaat tcgtgaatcc gcgcagggcg ctggtgcccg tgaagtcttc
     9421 ctgattgaag aaccgatggc tgccgcaatt ggtgctggcc tgccggtttc tgaagcgacc
     9481 ggttctatgg tggttgatat cggtggtggt accactgaag ttgctgttat ctccttgaac
     9541 ggcgtggttt actcctcttc cgtgcgcatt ggtggtgatc gtttcgacga agctatcatc
     9601 aactatgtgc gtcgtaatta cggttctctg atcggtgaag ccaccgcaga acgtatcaag
     9661 cacgaaatcg gttcggctta cccgggcgat gaagtccgtg aaatcgaagt tcgtggccgt
     9721 aacctggcag aaggtgttcc acgcggtttt accctgaact ccaacgaaat cctcgaagca
     9781 ctgcaggaac cgctgaccgg tattgtgagc gcggtaatgg ttgcactgga acagtgcccg
     9841 ccggaactgg cttccgacat ctccgagcgc ggcatggtgc tcaccggtgg tggcgcactg
     9901 ctgcgtaacc ttgaccgttt gttaatggaa gaaaccggca ttccagtagt tgttgctgaa
     9961 gacccgctga cctgtgtggc gcgcggtggc ggcaaagcgc tggaaatgat cgacatgcac
    10021 ggcggcgacc tgttcagcga agagtaatcg gatgcaggca ggggaaatgt ctgtttaccc
    10081 tgcctggtct gatacgagaa tacgcataac ttatgaagcc aatttttagc cgtggcccgt
    10141 cgctacagat tcgccttatt ctggcggtgc tggtggcgct cggcattatt attgccgaca
    10201 gccgcctggg gacgttcagt caaatccgta cttatatgga taccgccgtc agtcctttct
    10261 actttgtttc caatgctcct cgtgaattgc tggatggcgt atcgcagacg ctggcctcgc
    10321 gtgaccaact agaacttgaa aaccgggcgt tacgtcagga actgttgctg aaaaacagtg
    10381 aactgctgat gcttggacaa tacaaacagg agaacgcgcg tctgcgcgag ctgctgggtt
    10441 ccccgctgcg tcaggatgag cagaaaatgg tgactcaggt tatctccacg gttaacgatc
    10501 cttatagcga tcaagttgtt atcgataaag gtagcgttaa tggcgtttat gaaggccagc
    10561 cggtcatcag cgacaaaggt gttgttggtc aggtggtggc cgtcgctaaa ctgaccagtc
    10621 gcgtgctgct gatttgtgat gcgacccacg cgctgccaat ccaggtgctg cgcaacgata
    10681 tccgcgtaat tgcagccggt aacggttgta cggatgattt gcagcttgag catctgccag
    10741 cgaatacgga tattcgtgtt ggtgatgtgc tggtgacctc cggtctgggc ggtcgtttcc
    10801 cggaaggcta tccggtcgcg gttgtctctt ccgtaaaact cgatacccag cgcgcttata
    10861 ctgtgattca ggcgcgtccg actgcaggac tgcaacgttt gcgttatctg ctgctgctgt
    10921 ggggcgcgga tcgtaacggc gctaacccga tgacgccgga agaggtgcat cgtgttgcta
    10981 atgaacgtct gatgcagatg atgccgcagg tattgccttc gccagacgcg atggggccaa
    11041 agttacctga accggcaacg gggatcgctc agccgactcc gcagcaaccg acgacaggaa
    11101 atgcagctac tgcgcctgct gcgcccacac agcctgctgc taatcgctct ccacaaaggg
    11161 ctacgccgcc gcaaagtggt gctcaaccgc ctgcgcgtgc gccgggaggg caatagtggc
    11221 gagctatcgt agccagggac gttgggtaat ctggctctct ttcctcattg cgctgttgct
    11281 gcaaatcatg ccctggccgg ataacctgat tgttttccgg ccaaactggg tgttactcat
    11341 cttgttgtat tggatcctgg ccttgcctca tcgcgtaaat gtgggcacag gttttgtgat
    11401 gggtgccata ctggatctga tcagcggctc gacgcttggc gtacgcgtat tggcgatgag
    11461 catcattgct tacctggtgg cgctgaaata ccagcttttc cgcaacctcg cattatggca
    11521 gcaggcgctg gtcgtcatgt tgctttcgct ggtggtggat attattgttt tctgggcaga
    11581 gtttttagtg attaacgtct ctttcagacc ggaagtgttc tggagtagtg tagtcaatgg
    11641 ggtgctctgg ccgtggattt tcttgctgat gcgtaaagtc cgtcagcagt ttgcagtgca
    11701 ataaaggttt ctatgacttc tctgtattta gcttccggtt ctccgcgtcg tcaggagtta
    11761 cttgcgcaac ttggtgtgac ctttgaacgt attgttacgg gcattgagga gcagcgtcag
    11821 ccgcaggaga gcgcgcagca gtatgttgtg cgtctggcgc gcgagaaagc acaggcgggt
    11881 gtcgcgcaaa cggcgcagga tctcccggtg ctgggtgcgg atactatcgt tatcctgaat
    11941 ggagaagtgc tggagaaacc gcgcgacgca gagcatgcgg cgcagatgtt gcgcaaatta
    12001 tcgggtcaga cccatcaggt gatgacggca gtggcgttgg ccgacagcca gcacattctc
    12061 gattgcctgg tggtcaccga tgtgactttc agaacgttaa cagacgaaga catcgcgggc
    12121 tatgtcgcca gcggtgaacc gttagataaa gcaggtgcat acggtattca ggggctgggt
    12181 ggctgttttg tcaggaagat aaatggcagc tatcacgccg tagtcggctt acctctggtt
    12241 gaaacgtatg aattattaag taattttaac gcactgcgtg agaaaaggga taaacatgac
    12301 ggctgaattg ttagtaaacg taacgccttc ggaaacgcga gtggcgtata ttgatggcgg
    12361 tattctgcag gaaattcata ttgaacgtga ggcgcgacgc ggaatagtag gcaatatcta
    12421 caagggtcgt gtaagtcgtg tacttccggg tatgcaggcg gcttttgtag atattgggct
    12481 ggataaagcc gcgtttcttc atgcatccga tatcatgccg cacaccgaat gtgtggcggg
    12541 tgaagaacaa aagcaattca cggtgcgcga catctcggaa ctggttcgtc aggggcaaga
    12601 tctgatggtg caggtggtga aagatccgct tggcactaaa ggtgcgcgcc tgaccaccga
    12661 tatcacgctg ccttctcgct atctggtctt tatgccaggg gcttctcacg ttggggtttc
    12721 ccaacgtatt gaaagcgaat cagaacgtga acgcctgaaa aaagtggtcg cagagtattg
    12781 cgacgagcag ggcgggttta tcatccgtac cgcagcggaa ggggttggcg aggctgaact
    12841 ggcctccgat gccgcttatc tgaaacgcgt ctggaccaaa gttatggagc gtaaaaagcg
    12901 cccgcagacc cgttatcagc tgtacggcga actggcgctg gctcagcgtg ttctgcgtga
    12961 tttcgccgac gccgaactgg accgcattcg cgttgactca cgcctgactt acgaagcatt
    13021 acttgagttc acctcggagt acattcccga gatgaccagc aagctggagc attacaccgg
    13081 acgccagccg attttcgatc tctttgatgt cgaaaacgaa atccagcgag cgctggaacg
    13141 caaagtagaa ctgaaatccg gtggttatct cattatcgac cagaccgaag cgatgaccac
    13201 cgtggacatc aataccggag cgtttgtcgg tcatcgcaat ctggacgaca ccattttcaa
    13261 taccaatatt gaagcgacgc aggctatcgc tcgccagtta cggttgcgta atctgggcgg
    13321 gattatcatt attgatttca tcgatatgaa taatgaagat caccgccgcc gcgtgctgca
    13381 ctcgctggag caggcgttga gcaaagaccg ggtgaaaacc agcgttaatg gtttttcggc
    13441 gctggggctg gtggagatga cgcgtaaacg cacccgcgaa agcattgagc acgtactgtg
    13501 taacgaatgc ccaacctgcc acggtcgcgg aacggtgaaa accgtggaaa cggtatgcta
    13561 tgaaatcatg cgcgagattg ttcgtgtcca ccatgcttac gactccgacc gtttcctggt
    13621 ctatgcttct ccggcagtag ccgaagcctt gaaaggcgaa gagtcacact cgctggcgga
    13681 agtggaaatt ttcgttggca aacaggttaa agtacaaatt gaaccgctct ataaccagga
    13741 gcagtttgac gtcgtaatga tgtaaacaga tgctgggccg ccatccggca aagggttttt
    13801 gagtcacatt tttagcagac aaggagtgac gggtgaggcg attgccgggg attttactgc
    13861 ttactggagc cgcgctcgtt gtgatcgctg ccctgctggt tagcggcctg cgtattgctt
    13921 taccgcatct tgacgcctgg cgtccggaaa tcctcaacaa aatagaatcc gcgactggca
    13981 tgccggtaga agccagtcag ctctcagcca gctggcagaa ttttggcccg acgcttgaag
    14041 cacacgacat ccgtgcagaa ctaaaagatg gcggcgaatt ttcggttaaa cgcgttactc
    14101 tggcgctgga tgtctggcag agcctgttac atatgcgctg gcagtttcgc gacctcactt
    14161 tctggcagct gcgctttcgc accaacactc ctatcaccag cggtggtagc gatgacagtc
    14221 tggaagccag tcacatcagc gatctgtttc ttcgtcaatt tgaccatttc gatctccgcg
    14281 acagtgaagt cagtttcctg acgccatccg gtcagcgtgc cgagctggcg atcccacaac
    14341 tcacctggct gaacgatcca cgtcgacacc gtgcggaagg cctggtaagc ctctccagcc
    14401 ttaccggaca gcacggcgtg atgcaggtgc gcatggattt gcgcgatgat gaggggttgt
    14461 taagcaatgg tcgcgtctgg ctacaggcgg atgacatcga cctgaagccg tggctcggta
    14521 aatggatgca ggacaatatt gctctggaaa cggcacagtt ctcccttgaa ggctggatga
    14581 cgatcgacaa aggcgatgta accggcggtg acgtctggct gaaacagggc ggtgccagct
    14641 ggttgggcga gaagcaaacg catacgctgt cggtggataa tctgaccgcg catattacgc
    14701 gtgaaaatcc gggctggcag ttctctattc ccgatacacg gatcacgatg gacggcaaac
    14761 cctggccgag cggagcattg acgctggcct ggataccgga acaggacgtt ggcggcaaag
    14821 acaataaacg cagtgacgaa ctccggattc gcgccagtaa tctggagctg gcaggcctgg
    14881 agggcatacg cccgctggcc gcgaaacttt cacctgcact gggtgatgtt tggcgctcca
    14941 cacaaccgag cggcaagatt aacactctgg cgctggatat cccgcttcag gcggcagaca
    15001 agacccgttt tcaggcatcg tggagcgatc tggcctggaa gcaatggaaa ttattaccgg
    15061 gtgcggaaca cttctccggg acgctttccg gcagcgttga aaatggtttg cttaccgcgt
    15121 cgatgaagca ggcaaagatg ccttacgaaa cggtattccg tgcgccactg gaaatcgccg
    15181 acggccaggc aactataagc tggctgaaca atgacaaagg tttccagctg gatgggcgta
    15241 atattgacgt taaagccaaa gccgtccatg cgcgcggcgg ttttcgttac ctgcaacctg
    15301 ctaacgatga accctggctg ggtattctgg ctggcatcag taccgatgat ggttcacaag
    15361 cctggcacta tttcccggaa aacttgatgg gtaaagacct ggttgattac ttaagtggcg
    15421 cgattcaggg cggtgaagcg gataacgcga cgctggttta tggtggcaat ccgcaactct
    15481 tcccctataa acacaacgaa ggtcagtttg aagtgctggt gccgctgcgc aacgcgaagt
    15541 ttgccttcca gccggactgg cctgcattaa ctaaccttga tattgaactg gactttatta
    15601 acgacggttt atggatgaaa accgatggcg ttaatctggg cggcgtgcgc gcgagtaatc
    15661 tcaccgcagt gatccctgac tactcgaaag aaaaactgct gattgacgct gacattaaag
    15721 gtccgggtaa agccgttggc ccttactttg atgagacacc gctgaaagat tctctgggtg
    15781 cgaccctgca agaactccag ctcgacggcg atgtgaatgc tcgcttacat cttgatatcc
    15841 cgctgaacgg cgagctggta accgcgaaag gtgaagtgac gctgcgtaat aacagtctgt
    15901 ttatcaaacc actcgacagc accctgaaaa atttgagcgg taaattcagc tttatcaatg
    15961 gcgatctgca aagtgaacca ctgacagcaa gctggtttaa tcagccgttg aacgtggatt
    16021 tttccaccaa agaaggggca aaagcctacc aggttgcggt gaatctcaac ggtaactggc
    16081 aaccggcgaa aaccggcgtt ctgcctgaag cggtgaacga agcattgagt ggcagcgtgg
    16141 cgtgggatgg taaagtgggc attgatctgc cttatcatgc tggcgcgacc tataacgtag
    16201 agctgaacgg cgatctgaat aatgtgagta gtcacttacc ttcaccgtta gccaaacctg
    16261 cgggtgaacc actagcggta aacgttaagg ttgatggcaa tctcaacagc tttgaattaa
    16321 ccggacaggc tggtgcggat aaccatttca atagccgctg gttgcttggt caaaagctga
    16381 cgctcgatcg tgctatttgg gcggcagaca gtaaaacgct cccgccgttg ccggaacaaa
    16441 gtggcgttga actcaatatg ccgccgatga atggtgccga gtggctggcc ctgttccaga
    16501 aaggcgctgc ggagagtgtc ggtggtgcag cgagtttccc acaatacata acgttacgta
    16561 cgcctatgtt gtcactggga aatcagcaat ggaataacct gagtattgtt tcgcaaccga
    16621 cggcaaatgg caccctggtt gaggcgcaag ggcgtgaaat caacgccacg ctagcgatgc
    16681 gtaataacgc gccgtggctg gcgaatatca aatatcttta ttacaacccg agcgtggcga
    16741 aaactcgtgg tgattcaacg ccgtcatcac ctttcccgac aacggagcgc attaacttcc
    16801 gtggctggcc ggacgcacaa atacgatgca cagagtgctg gttctggggg caaaaattcg
    16861 gtcgcattga cagtgatatc accatttctg gcgatacgtt aacgctgacc aatggactga
    16921 ttgatactgg tttctcgcgg cttactgccg atggtgaatg ggttaataat ccggggaatg
    16981 aacgtacctc gctgaaagga aaactgcgcg ggcagaaaat tgatgccgcc gcagaatttt
    17041 ttggtgtcac gacgcccata cgccagtcgt catttaatgt ggattacgat ttacactggc
    17101 gcaaagcacc ctggcagcca gatgaagcga cgttgaatgg catcattcat actcaactgg
    17161 gtaaaggcga aattaccgaa atcaataccg gacatgccgg gcaattgctg cgcttattga
    17221 gcgtagatgc cctgatgcgt aagctgcgtt ttgatttcag agacactttt ggcgaagggt
    17281 tctattttga ctccattcgc agcaccgcgt ggattaaaga cggggttatg cacaccgacg
    17341 acacgctggt ggatggcctg gaggcggata tcgccatgaa agggtcggta aatctggtac
    17401 gtcgcgacct gaatatggaa gcggttgtcg caccagagat ttctgcgacg gtgggcgtgg
    17461 ctgcggcttt tgcggttaac cccattgttg gcgcggcagt ttttgccgct agtaaagtgc
    17521 tggggccgct gtggagcaaa gtctccattt tgcgctatca catttcgggt ccgctggacg
    17581 atccgcaaat caacgaagtg ttgcgccaac cgcgtaaaga aaaagcgcaa tgatttgacg
    17641 agggcgcgta attgccccaa tctcatatga taatcgttgc caaaggccaa cgagccagaa
    17701 cataaccgta ggccggatag ggcgttcatg ccgcatccgg cagccgtaaa aaatcctcta
    17761 ctgcagtaac taacgagtag caaaaacgat gagtctgaac ctggtaagtg aacaattgct
    17821 agcggcgaac agcctgaaac atcaggactt gttcgcgatc ctcggtcaac tggccgaacg
    17881 tcgccttgat tatggcgatc tctattttca gtcgagctat cacgaatcct gggttttaga
    17941 agaccgcatt attaaagatg gttcttacaa catcgatcag ggcgttggcg tgcgtgcaat
    18001 cagcggtgaa aaaaccggat ttgcttacgc tgaccaaatc agcctgctgg cgctggaaca
    18061 gagtgcgcaa gcggcgcgca ccatcgtccg tgatagtggt gatggcaaag tacagacgct
    18121 gggcgcggta gagcatagcc cgttgtatac ctcggtagat ccgctgcaaa gcatgagccg
    18181 tgaagagaag ctggatatcc tgcgtcgcgt cgataaggtt gctcgcgaag cggacaagcg
    18241 cgtacaggaa gtgactgcca gcctcagtgg tgtctatgaa ttaattctgg ttgcggccac
    18301 cgacggcacg ctagcggcgg atgtccgtcc gctggtgcgt ctttccgtga gcgttctggt
    18361 cgaagaagat ggcaaacgcg aacgcggtgc cagtggcggc ggcggtcgtt ttggttatga
    18421 gttcttcctt gccgatcttg acggcgaagt ccgtgcggat gcatgggcaa aagaagcagt
    18481 gcgtatggcg ctggtcaatc tttctgccgt tgctgcacca gcgggcacca tgccggtagt
    18541 acttggcgca ggttggccgg gcgtgctgtt gcatgaagcg gtaggtcacg gtctggaagg
    18601 cgacttcaac cgccgcggca cttcagtatt tagtgggcag gtcggggagc tggtggcttc
    18661 agaactgtgt accgtggttg atgacggcac gatggtcgat cgccgtggtt cggtggcgat
    18721 tgatgacgaa ggtacgccag gccagtacaa cgtgctgatt gagaacggca ttctgaaagg
    18781 ctacatgcag gataaactca acgcgcgttt gatggggatg acgccgactg gcaacggtcg
    18841 ccgtgaatcc tacgcccatc tgcccatgcc gcgtatgact aacacctata tgctgccggg
    18901 taaatcgacc ccgcaggaaa ttattgaatc cgttgagtac ggtatctatg caccgaactt
    18961 tggtggcggt caggtggata tcacctccgg caaattcgtt ttctccactt cagaagcata
    19021 tctgattgaa aacggtaaag taacgaagcc ggtgaaaggc gcaacgttga ttggttccgg
    19081 tatcgaaacc atgcagcaga tttcgatggt tggcaacgac ctgaaactgg ataacggcgt
    19141 gggtgtctgc ggtaaagaag ggcaaagttt gccggttggc gtgggccagc caacgctgaa
    19201 agttgataac ctgactgttg gcggtactgc gtaataattt cattattttc agaaggataa
    19261 ttaatctttc tacgtgcacg aacggtcccc tcgctcctct ggggttaggg tgaggggaac
    19321 ccgttggcac aggtttgtag aacgtaacag tacaatatga attacttctc tttcccgcgc
    19381 ccgtgcatct cctgaaacaa tttaccgacc tcaacaaaat aatccgtcag cgagttgatc
    19441 acgacctgta ccttcagcgg cagcttatct ttttcggtat ataacgcata aaccgggcgt
    19501 ggatctgact gataacgcgg cagcaggatc tccagctcac cgcgattgat ctcgttgatc
    19561 acccacatca gcggcacgta ggcgatcccg gcacccgccg tcagccagcg caccagcgtc
    19621 atcggatcat tagtcacaaa tcttccttgt gggatcaggc gagtcgagat cccttccggt
    19681 gcgatcagtt caaattcatt gtcgggccgc acgctgtatt caagccatga atgactactc
    19741 aaatcggcgg gtttttccgg tatgccgtat tgtgtgagat agcttttcgc ggcgcacacc
    19801 accatcggca tcgcgcccag acggcgggaa aacaggctgg aatcctgcaa cgcgccgacg
    19861 cggatcacca catccagacc gtcggcaatc aggtcggggg ctggaattcc ggtaaccaga
    19921 ttgacgctca aacctgggta ttctttcagc atttttgctg tcagcccggc gagaacattt
    19981 tgtgccatag ttgaagaaca gccaatgcgc agcgtcccga tgggggtgtt attgaaggca
    20041 tacagttgct catgaacatc ctgcacttca tgaagcatcc gacggcagcc ctggtagtaa
    20101 attctgccgg cttcggtcag gccaatgctg cgtgtgctac ggtttaacag ctttacctgc
    20161 aactcatctt ccagttttga caccgtctga ctgatggacg aaacgctcat ctgtagctgt
    20221 ctggcggcgg cggtaaaaga gccaaattca actactttgg caaacaccga catgcgtttt
    20281 agtcgttcca ttattcactc tgacttaaaa gtgatttaga tcacataata tagataacag
    20341 cataacagtt acgctaatat attaaatatc aatctacagc aatgttgctc tcgcccggct
    20401 ttccatgccc ttctctgcgg cgacagatgc tgaaaataat aacgcctgct ctctctttac
    20461 aaccaaggtc aacatgagtc tgtttcccgt tatcgtggtg tttggactgt ccttcccacc
    20521 gatatttttt gaattgcttt tatcactggc gattttctgg ctggtgcgcc gggtacttgt
    20581 gccaacaggt atctacgact ttgtctggca tccggcgttg ttcaacaccg cgctctattg
    20641 ctgcttgttt tatttgatat cgcgactgtt cgtttgaggt tgaagtgaaa acactaataa
    20701 gaaaattctc ccgtacggcc atcacggtcg tattagtcat tctagccttc atcgcaattt
    20761 ttaatgcctg ggtctattac accgaatccc cctggacgcg tgacgcgcgc tttagcgctg
    20821 acgtcgttgc gatcgcgccg gacgtttctg gactcattac ccaggtgaat gttcatgata
    20881 accagctggt gaaaaaagga cagatactgt tcaccatcga ccagccgcgc tatcaaaagg
    20941 cgcttgagga agcgcaagcc gatgttgctt attatcaggt actggcacag gagaaacgcc
    21001 aggaggccgg acgtcgtaac cgtctcggtg tgcaggcgat gtctcgcgaa gagatcgacc
    21061 aggccaacaa cgtactacaa acggttctgc atcagttagc gaaagcgcag gcgacccgcg
    21121 atctggcaaa actggatctt gaacgcacgg tgatccgcgc gccagcagat ggctgggtga
    21181 ccaacctcaa cgtctatacc ggtgagttta ttactcgagg atcaacggcg gttgcgctgg
    21241 tgaaacagaa ctccttctat gtactggcct atatggaaga aactaagctg gaaggggggc
    21301 gtccggggta tcgtgcagag atcacgccgc ttggcagtaa caaagtgctg aaagggactg
    21361 ttgatagtgt tgccgcaggg gtcaccaacg ccagcagcac gcgtgacgac aaagggatgg
    21421 cgactataga ctctaatctt gaatgggtgc gtcttgcgca acgtgttccg gttcgtattc
    21481 gtctcgacaa ccagcaagag aacatctggc ctgcgggcac cactgctaca gtggtggtca
    21541 ctggcaaaca agatcgcgac gaaagccagg attcgttctt ccgtaaaatg gcccatcgcc
    21601 tgcgtgagtt tggttaatca cgatgggtat tttctccatt gctaaccaac atattcgctt
    21661 tgcggtaaaa ctggcgaccg ccattgtact ggcgctgttt gttggctttc acttccagct
    21721 ggaaacgcca cgctgggcgg tactgacagc ggcgattgtt gccgccggtc cggcctttgc
    21781 tgcgggaggt gaaccgtatt ctggcgctat tcgctatcgt ggctttttgc gcatcatcgg
    21841 cacatttatt ggctgtattg ccggactggt gatcatcatt gcgatgatcc gcgcaccatt
    21901 attgatgatt ctggtgtgct gtatctgggc cggtttttgt acctggatat cctcgctggt
    21961 acgaatagaa aactcgtacg cgtgggggct ggccggttat accgcgctga tcattgtgat
    22021 caccattcag ccggaaccat tgcttacgcc gcagtttgcc gtcgaacgtt gtagcgagat
    22081 cgttatcggt attgtgtgtg cgattatggc ggatttgctc ttttctccgc gatcgatcaa
    22141 acaagaagta gatcgagagc tggaaagttt gctggtcgcg caatatcaat taatgcaact
    22201 ctgtatcaag catggcgatg gtgaagttgt cgataaagcc tggggcgacc tggtgcgacg
    22261 caccacggca ctacaaggca tgcgcagcaa cctgaatatg gaatcttccc gctgggcgcg
    22321 ggccaatcga cgtttaaaag cgatcaatac gctatcgctg acgctgatta cccaatcctg
    22381 cgaaacttat cttattcaga atacgcgccc ggaattgatc actgatactt tccgcgaatt
    22441 ttttgacacg ccggtagaaa ccgcgcagga cgtccacaag cagctcaaac gcctgcggag
    22501 agttatcgcc tggaccgggg aacgggaaac gcctgtcacc atttatagct gggtcgcggc
    22561 ggcaacgcgt tatcagcttc tcaagcgcgg cgttatcagt aacacaaaaa tcaacgccac
    22621 cgaagaagag atcctgcaag gcgaaccgga agtaaaagta gagtcagccg aacgtcatca
    22681 tgcaatggtt aacttctggc gaaccacact ttcctgcatt ctgggcacgc ttttctggct
    22741 gtggacgggc tggacttccg gcagtggtgc aatggtgatg attgcggtcg tgacgtcact
    22801 ggcaatgcgt ttgccgaatc cacgcatggt ggcgatcgac tttatctatg ggacgctggc
    22861 cgcgctgccg ttagggctgc tctacttttt ggtgattatc cctaataccc aacagagcat
    22921 gttgctgctg tgtattagcc tggcagtgct gggattcttc ctcggtatag aagtacagaa
    22981 acggcgactg ggctcgatgg gggcactggc cagcaccata aatattatcg tgctggataa
    23041 cccgatgact ttccatttca gtcagtttct cgacagcgca ttagggcaaa tcgtcggctg
    23101 tgtgctcgcg ttcaccgtta ttttgctggt gcgggataaa tcgcgcgaca ggactggacg
    23161 tgtactgctt aatcagtttg tttttgccgc tgtttccgcg atgactacca atgtggcacg
    23221 tcgtaaagag aaccacctcc cggcacttta tcagcagctg tttttgctga tgaataagtt
    23281 cccaggggat ttgccgaaat ttcgcctggc gctgacgatg attatcgcgc accagcgcct
    23341 gcgtgatgcg ccgatcccgg ttaacgagga tttatcggcg tttcaccgac aaatgcgccg
    23401 cacagcagac catgtgatat ctgcccgtag cgatgataaa cgtcgtcggt actttggcca
    23461 gttgctggaa gaactggaaa tctaccagga aaagctacgc atctggcaag cgccaccgca
    23521 ggtgacggaa ccggttcatc ggctggcggg gatgctccat aagtatcaac atgcgttgac
    23581 cgatagttaa gtcaaaaccg acgccaaaag cgtcggtttt ttcatggcta tacttagcga
    23641 tgcacggcag aactcgccgc gaaacgtgac ggtggcaaca gatgaatatt tatacctttg
    23701 attttgatga gattgagagt caggaggatt tttatcgtga ctttagccaa acctttggtc
    23761 tggctaaaga taaggtacgc gatctcgact cactatggga tgtgttaatg aacgatgtcc
    23821 tgccgctacc acttgagatt gaatttgttc atctgggaga gaaaacgcgt cgccgttttg
    23881 gcgcgttaat attgctgttt gatgaggcag aggaagagct ggaagggcat ttgcgtttta
    23941 atgttcgtca ttagcgcaaa aaaaagcccc cgaaccgggg gcaatatcgt cggacaagac
    24001 gatgagggtt tatttgtaca gttcagccgt agcgtgccag gtgtcaccgc tacgagcttc
    24061 agtaatctgg taggccgttg cgcctttctc ttccgctttt ttgttcagca tttcacgcat
    24121 atccattggc gaagacgcca caccacttac ggatacggtc ccgattgctt cacgattttg
    24181 tgcttgtgca gtatcaatgg agtcggcagc gaatgcaccg aaagagagaa cagaaagtac
    24241 gcttaatgca gctacagtgg ttttgatttt catgattttt acctcgacat aatcttttag
    24301 ctgggccttt gtttcgtgac ccatatcaca aaatcaagca tacactaatt attgaactaa
    24361 ttaataccac
//
LOCUS       DAZPDU010000051        24349 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZPDU010000051 DAZPDU010000000
VERSION     DAZPDU010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24349
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.051"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            234..371
                     /locus_tag="ACHURS_001856"
                     /pseudo
     CDS             234..371
                     /locus_tag="ACHURS_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
     gene            complement(479..1714)
                     /locus_tag="ACHURS_001857"
     CDS             complement(479..1714)
                     /locus_tag="ACHURS_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFR1237608.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAARDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(1861..1937)
                     /locus_tag="ACHURS_001858"
     tRNA            complement(1861..1937)
                     /locus_tag="ACHURS_001858"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1901..1903),aa:Pro,seq:tgg)
     gene            complement(1980..2066)
                     /locus_tag="ACHURS_001859"
     tRNA            complement(1980..2066)
                     /locus_tag="ACHURS_001859"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2030..2032),aa:Leu,seq:cag)
     gene            complement(2087..2162)
                     /locus_tag="ACHURS_001860"
     tRNA            complement(2087..2162)
                     /locus_tag="ACHURS_001860"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2127..2129),aa:His,seq:gtg)
     gene            complement(2221..2297)
                     /locus_tag="ACHURS_001861"
     tRNA            complement(2221..2297)
                     /locus_tag="ACHURS_001861"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2261..2263),aa:Arg,seq:ccg)
     gene            complement(2400..3785)
                     /locus_tag="ACHURS_001862"
     CDS             complement(2400..3785)
                     /locus_tag="ACHURS_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR1237609.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3976..4716)
                     /gene="wecG"
                     /locus_tag="ACHURS_001863"
     CDS             complement(3976..4716)
                     /gene="wecG"
                     /locus_tag="ACHURS_001863"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFR1237610.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4719..6071)
                     /gene="wzyE"
                     /locus_tag="ACHURS_001864"
     CDS             complement(4719..6071)
                     /gene="wzyE"
                     /locus_tag="ACHURS_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFR1237611.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWMALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLIIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(6068..7147)
                     /gene="rffT"
                     /locus_tag="ACHURS_001865"
                     /gene_synonym="wecF"
     CDS             complement(6068..7147)
                     /gene="rffT"
                     /locus_tag="ACHURS_001865"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFR1237612.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(7144..8394)
                     /gene="wzxE"
                     /locus_tag="ACHURS_001866"
     CDS             complement(7144..8394)
                     /gene="wzxE"
                     /locus_tag="ACHURS_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFR1237613.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8396..9526)
                     /gene="rffA"
                     /locus_tag="ACHURS_001867"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8396..9526)
                     /gene="rffA"
                     /locus_tag="ACHURS_001867"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFR1237614.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9531..10205)
                     /gene="rffC"
                     /locus_tag="ACHURS_001868"
                     /gene_synonym="wecD"
     CDS             complement(9531..10205)
                     /gene="rffC"
                     /locus_tag="ACHURS_001868"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFR1237615.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(10183..11064)
                     /gene="rfbA"
                     /locus_tag="ACHURS_001869"
     CDS             complement(10183..11064)
                     /gene="rfbA"
                     /locus_tag="ACHURS_001869"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFR1237616.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     DIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(11083..12150)
                     /gene="rffG"
                     /locus_tag="ACHURS_001870"
     CDS             complement(11083..12150)
                     /gene="rffG"
                     /locus_tag="ACHURS_001870"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFR1237617.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(12150..13412)
                     /gene="wecC"
                     /locus_tag="ACHURS_001871"
     CDS             complement(12150..13412)
                     /gene="wecC"
                     /locus_tag="ACHURS_001871"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFR1237618.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13409..14539)
                     /gene="wecB"
                     /locus_tag="ACHURS_001872"
     CDS             complement(13409..14539)
                     /gene="wecB"
                     /locus_tag="ACHURS_001872"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFR1237619.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14595..15641)
                     /gene="wzzE"
                     /locus_tag="ACHURS_001873"
     CDS             complement(14595..15641)
                     /gene="wzzE"
                     /locus_tag="ACHURS_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFR1237620.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15653..16756)
                     /gene="wecA"
                     /locus_tag="ACHURS_001874"
     CDS             complement(15653..16756)
                     /gene="wecA"
                     /locus_tag="ACHURS_001874"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFR1237621.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16996..18255)
                     /gene="rho"
                     /locus_tag="ACHURS_001875"
     CDS             complement(16996..18255)
                     /gene="rho"
                     /locus_tag="ACHURS_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFR1237622.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18340..18441)
                     /gene="rhoL"
                     /locus_tag="ACHURS_001876"
     CDS             complement(18340..18441)
                     /gene="rhoL"
                     /locus_tag="ACHURS_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFR1237623.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18582..18911)
                     /gene="trxA"
                     /locus_tag="ACHURS_001877"
     CDS             complement(18582..18911)
                     /gene="trxA"
                     /locus_tag="ACHURS_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFR1237624.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            19042..20307
                     /gene="rhlB"
                     /locus_tag="ACHURS_001878"
     CDS             19042..20307
                     /gene="rhlB"
                     /locus_tag="ACHURS_001878"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFR1237625.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20441..21925
                     /gene="gppA"
                     /locus_tag="ACHURS_001879"
     CDS             20441..21925
                     /gene="gppA"
                     /locus_tag="ACHURS_001879"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFR1237626.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIANAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(21972..23993)
                     /gene="rep"
                     /locus_tag="ACHURS_001880"
     CDS             complement(21972..23993)
                     /gene="rep"
                     /locus_tag="ACHURS_001880"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFR1237627.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            24210..>24349
                     /locus_tag="ACHURS_001881"
     CDS             24210..>24349
                     /locus_tag="ACHURS_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1237628.1"
                     /translation="MAINFSPKVGEILDCNFGNYPVSQNGQFSTTYYDGRIPPEMIKN
                     RLV"
BASE COUNT         6130 a         6666 c         6008 g         5545 t
ORIGIN      
        1 aaactaaaaa agcattaata atcaacgcaa tataataaac ccgccttaca tatcaactgc
       61 gccagaggta ggattgaaaa cgctctcctg attttccaat tcattttctg gataaataaa
      121 taatttattt ttgtcactat tatttatgta atcatcctgt cagggagagg gatctcaatt
      181 atcaatgctt aattacgtca tcattttgat gacatgaaac cggagcattt tgcatggaat
      241 tttcgttttc acccaaactt cttgttgttg ctgtcgccgc cgctcttcct ctcatggcca
      301 gcgcagcaga taccccgtca actgccaccg cacgcaaagg ctttgccgga tacgatcacc
      361 caaaccagta tccacagaac gctgcaattt attgaatttg cacgatcatg taggccggat
      421 aaggcgttta cgccgcatcc ggcaatcaac cgcaggcggc cacaaaatgg ccgctttttt
      481 acttattcac caccagcaaa tgtgcctgca taatgtcact ggccgggcga ccgtgcgcca
      541 gcaaatcagc cattgcttta agatatggcg gtagatggcg gaaataacgc tgatacccgg
      601 cacacaaata attcagcccc ggtttgccgc tggcatcgag catgaagcgg tgtttcgggc
      661 agcctcccca gcacgctttt aacacgttac aactgcgaca ctgcgccggt aattgcttaa
      721 atttatcttc accaaacacc tgctgttgcg gggaatcgat catttctgca attgtttgct
      781 gatgcatatt ccccagccga tattgcggat aaacatagtg atcgcaggcg taaacgtcgc
      841 cgttgtgctc aacaatcacc gagcgcccac aggttggctg atgatggcaa accgcacccg
      901 gcgcaccgac aaaattggca aacgcccatt cgatattcat cacgaaaatc ttgccgacgt
      961 cgcgtttgat ccagtggtcg aatatcgcca ccagaaattc accaaattca tcggggcgca
     1021 ccgaccattc cgtcagttcc ccctgaatat caccaggcgc atgcaatttc agtccatcgc
     1081 gggcagctgt ttcatcagcc agacgctcga ccaccggaat aaactggatg aattcgactc
     1141 ccgcatcgca caaaaaatca taaacctgca acggttgctg cgcgctggtg cggttgacgc
     1201 agaccagcac gttatagtcg acatgatgtt tttgcagcag cgtcagggca cgcatcacca
     1261 gcttatgcgt tggtctgcca cctttggtca cgcgatattg attgtggatc tcagccggac
     1321 cgtccagcga taacccaaca agaaaatgat tttctgccag aaatgcacac catttatcat
     1381 cgagcagcac gccgttagtc tggaagctgt tacttatctt cctgccagca ccgtatttcg
     1441 cctgtaactt tacggcacgg cggtaaaaat ccagcccgag taacgttggt tcgccgccct
     1501 gccaggtaaa agccacttcg ttttgcggtt cgctggcagc gatatagtga cggacatacg
     1561 cttccagcgt gtcatcgtcc atatgcgtga ctggcttttc gcggtaaagg gattgttttt
     1621 cgagataaaa acagtagtca cagttcagat tacaatcgga accactcggt ttcgccatca
     1681 catgaaaagc acgcgttgga acctgttgca gcatggtcac tccttgcctg aagtcatcct
     1741 tcaatacgtt aagggcgaaa tcataccgtg gggcaatgag ttattacggg cgtttattgc
     1801 gaagggaggg tgacgaaatg cacagaaaac aaaaaacccc gccgaagcgg ggttcaaaat
     1861 tggtcggcga gagaggattc gaacctccga cccactggtc ccaaaccagt tgcgctacca
     1921 agctgcgcta ctcgccgaaa tactgctttt tgaattttta gttcaattct ttaaagtcgt
     1981 ggtgcgaggg gggggacttg aacccccacg tccgtaagga cactaacacc tgaagctagc
     2041 gcgtctacca attccgccac cttcgcattg tcacaacttc taataatggg gtggctaatg
     2101 ggattcgaac ccacgacaac tggaatcaca atccagggct ctaccaactg agctatagcc
     2161 accactacaa atcttgttac gcggtattac taccaccgca gctcaagcgc cgggactaaa
     2221 tggcgcgccc gacaggattc gaacctgaga cctctgcctc cggagggcag cgctctatcc
     2281 agctgagcta cgggcgctta gcgccgttgc gggggtggat aatacggact tcccacccct
     2341 ctgtctagtc cctttttaaa taaattgcgc gtttggttac ggtttgtgcg ttttgctgct
     2401 tattcctcca gtttatgcgc tttcccgtgg cgattaaggc caaaaacttt ataaatcgcc
     2461 gtcaccgcca gcataaagat gatgccaaca aacagcgaca tacgcgtatc ttcattaaag
     2521 tacatgccga tcaaaacgca aatcaggaat gccattgtta cgtagttggc ccacgggaac
     2581 aggattgagc ggaacggatg gctggcaatc gccgctttat gtgcacgccg aaaacgcagc
     2641 tggcttatca atatcacaaa ccatggcacc atccccggaa gcacgctggc actgtagacg
     2701 tagacaaaca cacgctgcgg attgggaatg atgtagttca ggcatgagcc aattagcaga
     2761 atagcaatag atactgccac acccgcaact ggtacgccgt gacgggaaac tttcgccatc
     2821 gccgccggta actgacggtt tttcgccagt gcgtagagca tacgtccgca actgtacatg
     2881 ccgctgttac agccagagag cgcagccgtc agcaccacaa agttgataat gcccgccgct
     2941 gcggtaatac cgattttggc aaaagtcagt acgaacgggc tgccgttgct gccaatttca
     3001 ttccacggga agatggtgac gataacgaaa atcgcgccta cgtagaaaat caggatccgc
     3061 cacagcactt tgcctacagc actgcgcagc gtcacctgcg gattcttcgc ttcaccggca
     3121 gtaatgccaa tcagctccac gccctggtag gacgccacca caatacacag cgcggtcagg
     3181 aaccctttcc agccacccgc aaagaaaccg ccatgctctg tgagattgct aaaaccaatc
     3241 gactgcccgc cattgccaaa gccaaagaaa atcacgccca ggccaatgac aatcatcacg
     3301 ataatcgtgg tgactttgat catcgcgaac cagaactcga tttcgccgta caaccgcacc
     3361 gccgccagat tcgccaacgc caccagcgcc accgcgatca atgcaggtat ccactgcgcc
     3421 atctccggga accagaactg gacataaacg ccaatggcgg tgatttctga gatccccacc
     3481 gccatccaca taaaccagta agaccaggcg gtgagataac caaaaaacgg gctcatataa
     3541 cgatgcgcat aaacggcgaa cgaaccggta acaggttcaa ggaacaacat ttcgcccatt
     3601 gaacgcatga tgaaaaagac gaacagcccg gcgatgatat aggccaacaa tacggatggc
     3661 ccggcccatt tcagggtact ggcggccccc ataaacaggc cgacgccaat agtgcccccc
     3721 agggcgatga gttcgatatg tcgagcttcc agcccacgct gtagctctgg tttgttatct
     3781 gccataaatc ctcgtgttgt gtttgcatgc tttccggtgt taccggttat cgttatgggt
     3841 acatcgagtg ttgcgaatgt tttcgtaatt caggagaaat ggcaaataaa gcattaaaaa
     3901 tttgaatgct ttgtgtaata aaaaagcaga caggcgacgg agtgaccact ccgtcgcttt
     3961 acaaagagag gaaaatcaga ggttgccggt gtagtgccag cgtaaataac gcagcaaacg
     4021 aagctgacgc ttaatgcggc tcggctgcga aagcaggcgg tagagccact ccagtcccag
     4081 cgtttgccag attttcggtg cgcgttttac gtgaccggtg aaaacatcgt aagtcccgcc
     4141 aacgcccata tacagcgcat ctggatgcac cagacggcag tcgcgcatga agatctcctg
     4201 ctttggcgat cccatcgcaa cggtgacgat ttgcgctccg ctggcatgaa tgcgttcaaa
     4261 cagcgcctga cgctgctcgg gtttaaaata accatcctga ctgccaacga tattcacatt
     4321 ccattggttg cgcagtttag cttcagtttg cgccagcact tcaggtttac cgcccacaag
     4381 aaataccggc gtcccttctt tgcctgcgcg cgccatcagc tcttcccaga gatcggcacc
     4441 ggcaacgcgg gaaacctgcg cctgcgggta ctttttacgt actgaacgta caacgctgat
     4501 gccatccgca tatttaaatt cggcagcgtt aattaactcc ctgacctcgg cgttatcttc
     4561 aatggtcagc attttttcag cattaatggc aaccagcgtt ccctgcttaa gctgcccgtc
     4621 agcaaacaga taatcgaggg cgtgctgcat atcacgccaa ccaatcaact gtaagccacg
     4681 cagcgtatag gtcggtgccg tggtgttgtt attcattgtt atccttcaac ctgcgtccgg
     4741 agcgatgatt ttgtacgttt atgaatgagt ccggcgcttt caaaaagcca gtacagcagt
     4801 tttgcgatta tcagacatgc gccgaagacc acgataaaaa agactacgcg tgagacaaac
     4861 gaatccagcc cttcacgcgc cagcacgatc atattgaaga tcgccccaaa gcagaaactg
     4921 tgcaatatcg cagccttata gcgattaggc tcgcggttgc ccagctcata cagccagtcg
     4981 aaccatttga tgatcagacc aaccacgatc gccccgagcg ggatgaacaa cgcgccgccc
     5041 atcaccacca gtgagcctat aagcgtaggc gagatcgcca gtccggagtg gttattcagc
     5101 acttcccagg taaagtagtt ggctgagttc agcaccatac tcgggcgacc cggccacagc
     5161 caggaaggga taaagacata gaaatcgcgg acaattggag ccaggccctg gaagtcgatg
     5221 ttgtcgtagt tctgcaacag caacgccaga ttctcccacg gggagaaggt gtcgcgagtg
     5281 agatagagaa acgtatagaa cgcttcatcg ccgctcacat tcattccata gcgttttagc
     5341 gccatccaga acatgccaac aatccccagc acgcccgccg ccgccagcat ccacaacgaa
     5401 atccagccgc gaataatgcc aataaacagg aagatagcga atgcgatgat gatattggcg
     5461 cgagtgccgc cgacaatcat ataagtcagc aagccaaagg cgaccgtgct gacgaggaaa
     5521 aacagccacg ctttgctgtc ctggcgcaga aagtagacca ccagcatcgc cgggatgaaa
     5581 aagtaaaaga agcgttttaa cgccacgccg gagacttcac tggagaagat ctgactgtag
     5641 gagttaagcc ggaacagcaa aaagccgttg tgcatgaaga agatgccgac gcttaccagc
     5701 gcgataccca tcaggatcac ccacgtaaga ttggtctcca cgcggttcat ggtaaacagc
     5761 ggacggcgcg gtacatcagc aacgcgtttg cgtaggcggg ttttgtaggt gacatagtaa
     5821 accgcgtaga agcagcccgc agaaagcaac gcctgcaaca agatttctgg cggcgcgaca
     5881 ccaacatcaa agcgaaatac cagcacgctg gtcagcggga agccgaagaa aaaggtgagc
     5941 aaaaacagca atgaaaagaa gacattgaag ttaaagcgca cacggcgaaa ctcaaaccag
     6001 gtcagcgtgg caataaacag cgtgcagagc agccagacca caaacaggcc actgaattgc
     6061 agcagactca tgcgacctcc ccggcggcaa tcgccaacgc ccgctgccag ccttgtagat
     6121 agttagggct aaagaaggca atggtgtttt tatccaccga cgccaactgg cgctgcgctt
     6181 cacgcacaat atcctcgttg agatcgtcgg tagtaaacag caccggcaaa tgctgttccg
     6241 tcatatcctg ccagaacgga ttttcccggt taagcacaca aggaatgccc gcctgaatca
     6301 gtaagcaaag cgtaccaatg ccctgctggc gggcaaaaat aaagtaacca agatcgcact
     6361 gacgaagtag cgccagatag gcgtcaaatt ccagtttttc gctcaaaatt tgcagatttt
     6421 cttcgctgaa taactccagc cccgcctgac gaacttcctc aatgtacgct tcgttattag
     6481 gcggatatcc catcggcacc accactttta ccgtatcgcc aaattgctga tgaacggcgc
     6541 gcaaggcagc aatatggtca ttgctgcggt caccggagtt ccccaccaga atggtcattt
     6601 tcccttcacg ttgccgatca ttcgccatcg tattgagaga agggtccatc cgcgtcggga
     6661 agtacagcag ttcgccccgc acctttggat gcgttttggc aaaaaagctc aaatcaccgc
     6721 gggtggcaaa tacacagccg actcgctttt gcgccaggcg acgtagcggg taaaaaagct
     6781 tatatctcaa gccactggaa agctcataca ggtctgcccc ccagatatgc cagaaaaact
     6841 ggctgggctt aatgccacca ctcagcagag ccagccacag tgtgggattg aactgaccgt
     6901 ggaaaaaaaa tcgctgctga cggttagctt ttgctttcgc gatgaccgct tccgccagcg
     6961 attttttccc agggaaaaat tgcacagaaa gcgccggaca gctatcactt aagccgtcgt
     7021 ccttgccaac aaccataaac tcgcgcgcgt gctcgctcgt cgcggccagc gcgtcattga
     7081 aaaaccgcaa aacggttcgg ttatggtgag ggatatccga tcccagtacg tgaatcagta
     7141 cagtcatgcc cgcctacgcc agagtaaaaa cacgccacaa caaagagaaa aatagacgat
     7201 ataagttgcc atatatgcct gcgccgcgcc cagtgcacca tgcgcaggga ttagccagtg
     7261 ggcaaatacc atcaataaag tgaactggct gacttccgcc agaatataaa accgcagtga
     7321 cgctttggcg atcaccagat aaccaaagac ataagcgccc acttttaaca catcacccac
     7381 taactgccag gcaaagagat cgcgcatagc ggtaaattta ttcgacaaca gcaaccagat
     7441 agcaaaatca cgcagcaacc agacggtaaa actcgccgcc gccactgccg gtaagacgaa
     7501 tttcagcgat ttaaccactt cccgggtgat atcgcgcttt tccgttagcc gcgacaacgt
     7561 gggcagcaaa tatacgctga acgatgccgt aataaattgc aggtaggcat cggaaatact
     7621 gctcacccct tgccagatcc ccacttcatc ccagctatac tgcgccgcca gcagtttacg
     7681 catcatgatg taagcaacag gcaaggtcac cgacgtaatc aacgccatga gcgtaaattt
     7741 gctcaactgc cctgccagac cgttatccca gctgggtttc agatagctta acgggatgac
     7801 accacgtttg atcaacatga tggcggcagg aatcaccacc agcgcgggaa tcagcgccag
     7861 acccagcaac gccccttcat aaccgcccaa acggtaactg acgtagtacg cgagaacgcc
     7921 aatcaagctg ccgacaatca gcgataacgc attacctgcg gcatcgcgaa agcctttcat
     7981 cagcgccagt aacaggttgc cccaggcgat ccccatttgc accagcgcca ctaaacgcac
     8041 cagcccctga tagtcggtat taccaaacag tccctggctg attggcgcag ctgccagcac
     8101 aaaaaccagc gccatcagcg tagagaaacc aagtaccatc gctgatgaag tgccgaccac
     8161 gcggcgcagc tgttgcggat tatcatggta ctgggcaacg tatttggtta caccgttaaa
     8221 gatgccagct ccggcaagca cgccgagcac ggtaatcaac tggcggaaat ttgccgccag
     8281 cccaagcccc gccggaccaa atgacacagc cagcaactta ccgaccagta acccggcacc
     8341 aatcttgacc agtgtactgg ccgccgtcca caaggacgct tttgccaacg acatatcagg
     8401 agaagtagtt caacaaagtc gcaattaccg tacgctgatt gacgggcgac aggttgtaga
     8461 acagcggcag gcgcagcagg cgctcgctct ctttggtggt gtagcgatct tcaccgtgga
     8521 actcaccaaa gcgttccccc gcagggcaac cgtgcagcgg aatgtaatgg aacaccgcca
     8581 tgatttccgc ttctttcaga aagttaatca acgcgctccg gtcatcaata tcccgcagtt
     8641 taatgtagaa catatgcgcg ttctgcacgc agccatcggg aatcgacggc agctcgatac
     8701 gcccggcttt cgccagaggc gctaacgcat cgtagtagtt ttgccacagc gccagacgtt
     8761 gctggttgat acgatccgct gcttccagtt gcgcccacag gtacgcagct tgcagatcgg
     8821 acatcaaata gctggagcca atatcgcgcc aggtatattt atcgacctga ccacggaaga
     8881 actggctgcg gttagtgccc ttttcacgga tgatctcggc tcgttcgatt aacgctttat
     8941 cgttaatcag cgtcgcgccg ccttcaccac ccgccgtata gtttttggtt tcatggaagc
     9001 taaagcagcc aatatgacca atggttccca gtgcacgccc tttgtaagtg gacatcacgc
     9061 cctgagcggc atcttctacc acaaacagat tatgcttttt cgccaacgcc ataatggtgt
     9121 ccatttcgca ggccacaccc gcgtaatgga ccggcacgat aacgcgcgtt ttgtcggtga
     9181 tcgccgcttc aatcagcgtt tcgtcgatgt tcatggtgtc cgggcgaaca tccacaaaaa
     9241 cgatttttgc gccacgcagc acaaaggcat tggcggtgga gacaaaggtg tagctcggca
     9301 tgatcacttc atcgccaggc tggatatcga gcagcagcgc cgccatctcc agcgaagcgg
     9361 tgcaggacgg cgtcagtaac actttggcgc tgccaaaacg ttgctccagc cactgctggc
     9421 agcgacgggt aaaaccgcca tcgccacaca gtttgccgct acccattgcc gactgcatat
     9481 agtcgagttc ggttcccacc accggcggtg cgttaaatgg aatcatgtga tcacctgtat
     9541 aaccagtacg cggtgctttc tacattcgca ccactttgta tgtatcgttt aagcgcggcg
     9601 gtgttgccca tttgggtcgc cacccgcaaa gttgttttac cgcgagcata cgcccagttt
     9661 agcgccgttt gcatcagctc agcacctgca ccgcgtccag ccagcaggcc aattcgcgca
     9721 tctgtcgcat tgagttcccg taaagagaca taaccgcgaa taccgccgga cgccgcacgt
     9781 aaaatcagac attgatgatc aaaggtgccg cgcacggcat tttcaatcca ctgtgcataa
     9841 aagcgtccgc tggcgtcagg cgcataccac ggcgcacgaa aacggctttg cgcaaatgcg
     9901 gcgctggcta actgacgtaa tgcgggaata tcggtctctt gtgccactac agcaccacta
     9961 tcactgacat tgttcacggg tagtgccaaa tcaacttcac cttctaccag ggagaatccc
    10021 agctgttgca gggcatccag ttcacccgta tttgatgccg caattttggc ctgcacccgt
    10081 gaccacggcg ctaacacgtc tggcgtcagg agcggtgctt cagacgtaat gcgcacgatg
    10141 gcgctgttaa caccaaagaa ggcgttttcc caggttagtg gctcaatact ggcgcggacg
    10201 ggcacgaagt aactccagca gatattggcc gtagccagtt ttcgctaatg aactggcagc
    10261 acgcttcaca ccctcgtcat cgagccagcc gttacgccag gcgatctctt ccaggcaggc
    10321 aatcttaaag ccctggcgtt tttctactgt ctgcacaaag gtactggctt caatcaggct
    10381 gtcgtgagtg ccagtgtcca gccaggcaaa tccgcgcccg agcagttcaa cggtcaggtt
    10441 gcccgcctcg aggtacatct ggttgatgga ggtaatctcc agttcaccac gctccgacgg
    10501 cttcacctgc tttgcgtact ccacgacttt actgtcgtag aaataaagcc cggtcaccgc
    10561 ccagtttgac ttcggctgtt ttggcttttc ttccagcgag atagcgcgga aattgtcgtc
    10621 aaactccacc acgccaaagc gttccgggtc catcacctga tagccaaata ccgtcgcccc
    10681 ttccgtgcgc gccgcaacat gacgcagctt cggactgaag ccctgaccga agaagatgtt
    10741 atcgcccagc accagacaag aaggttcacc gttgaggaag gtttcaccga tgataaaggc
    10801 ctgcgccaga ccgtccgggc tgggctgttc ggcgtattcc agctggatac cgaactcact
    10861 accatcgccc agcaggcgct ggaaataacc tttatcttcc ggcgtagtga tgatgagaat
    10921 ttcgcggata tcggccaaca tcagcaccga cagcggatag taaatcattg gcttatcgta
    10981 aatcggcaac agttgcttcg atacgccgcg cgtaatcgga tgcaatcggg tgccggaacc
    11041 gcccgccagg ataatacctt tcatgtttgc ctccgctgaa aattagcctt tcagacctaa
    11101 acgctcgccc tgatagctgc cgtcctgcac ctgcttccac cagctttcat tagccagata
    11161 ccactgcacc gttttacgca ttccactttc aaaggtttcc tgtggcagcc agccaagttc
    11221 acgggcaatt ttcgaagcat caatggcata acgcagatca tgccccggac ggtcagcgac
    11281 aaaggtgatc aagtcacgat agtgcaccac gccgtgcggc ttgttcggag ccagttcttc
    11341 cagcagctcg caaatggttt ccacaacatc gagattctta cgctcgttgt gaccaccaat
    11401 attataggtt tcaccgactt tcccggtggt cgccacgcaa tacagcgcgc gggcgtgatc
    11461 ttccacatac agccagtcac ggatttgctg cccgttgcca tataccggca gcggtttacc
    11521 cgccagcgcg ttgaggatca tcagcgggat cagtttttcc ggaaagtggt aaggaccgta
    11581 gttattcgag cagttggtga taagcgtcgg cagaccgtag gtccgcagcc aggcgcgcac
    11641 cagatggtcg ctgctggctt ttgacgcgga ataagggctg ctcggcgcat acggcgtggt
    11701 ttcggtgaag aaatcatccg tcgagtgcag gtcaccatat acttcgtcgg tggagatatg
    11761 atgaaaacgg aacgctgatt ttttatcttc cgtcagcgta ttccagtaag cccgcgccgc
    11821 ttcaagcaat gtataagtcc cgacaatgtt ggtttcaata aacgctgccg ggccgtcaat
    11881 agaacggtca acatggcttt cggctgccag atgcatgaca cagtctggct gatgctcagt
    11941 gaatacgcgt gccagttctg cccgatcgca gatatcaact ttctcaaagg caaagcgctc
    12001 gctttgcgcg accggtgcca gcgacatcag gtttccggcg taggtcagct tatcgaccac
    12061 taccaccgcg tcgctcgttt cgttgatgat ataacgcacc agcgccgagc caataaaccc
    12121 ggcaccacct gttatcagaa tttttctcat cagcgccaga ctcctttggc atcgacgaca
    12181 tactgctgat ggacattgtc gccattgata actttaaact gactatgatc gaccagcatc
    12241 accagcacgt ctgccgttgc cagcgcctcg tcaagctgcg ccagagtaca aagcccggtc
    12301 agttttttcg gcaactggtg gatgttaggc tcaacaacca gagtttcgcc gctatgccac
    12361 tgggcgatca gttcagcgat ttccatcgcc gggctttcgc gcaggtcatc aatattcggt
    12421 ttaaacgcca gaccaaagca ggcgattttc agttcactgg cgcgtttatc ggtagccgcc
    12481 aggcaatcag ccaccgccgc tttcacctga tcgataaccc agaacggttt gtgatcgttc
    12541 acttcgcgcg cggtacggat aagccgcgcc tgctgggggt tctgtgccac gataaaccac
    12601 ggatcaacag caatgcagtg accgcccacg ccagggccag gctgaagaat attaacgcga
    12661 gggtgacgat tcgccaggcg aatcagttcc cagacgttaa tcccctgatc ggcacaaatc
    12721 agcgacaatt cattagcaaa agcgatattc acatcgcgga agctgttttc ggtgagctta
    12781 cacatttccg ccgtccgcga gttagtgacg acacactcac cttcgaggaa aattttgtac
    12841 agttcgctgg cgcgggtcga acaaaccggc gtcataccac caatcacgcg atcgttttta
    12901 atcagctcga ccataacctg gcccggtaac acgcgttccg ggcagtaagc aatgttgacg
    12961 tccgcctgct cgcccacctg ctgtgggaaa gtgagatccg gacgcatctc tgctaaccat
    13021 tctgccatct tctcggttga ccccaccggc gaggtggatt caaggatcac cagcgcgcct
    13081 tttttcagca ctggcgcaat ggagcgagca gccgattcaa cgtaggtcat atctggctca
    13141 tgatcgcctt taaacggcgt gggtacagca atcagccagg catccgcttc aactggcgtc
    13201 gtgctcgctc gtaaaaaacc gccttctacg gcagttttta ctacgctcgc caaatcaggc
    13261 tcgacgatat ggatttcgcc acgattgatg gtatcaaccg cgtgttggtt gatatcgaca
    13321 ccaatcacct gtttttgccg tgaggcaaac gctgctgctg taggcagccc gatgtaaccc
    13381 agtccgataa cagaaatggt cgcaaaactc atagtgatat ccgattattt tttaacgctt
    13441 ccagaatgcg agagcatgcc tgaccatcac catacgggtt atgggcgcgg ctcatagttt
    13501 gatattcgtt ttcgtctttt aaaagacgcg tcacttcctc gacaattcgc tgcttatccg
    13561 tgcctaccag acgcaccgta cccgccgtca ccgcttccgg acgctcagtg gtatcgcgca
    13621 tcaccagcac cggtttcccc agcgaaggcg cttcttcctg aatgccgcct gagtcggtca
    13681 aaatcagcca ggcgtggttc atcagccaga caaacggtaa atactcctgg ggatcgatca
    13741 gaatgacatt tttcacatgc cccagaatgc gattgaccgg ctctctgacg ttcgggttga
    13801 gatgcaccgg atagacaatc tggatgtcct ggtgcgtggt ggcaatgtct gccaacgcgt
    13861 ggcagatttc ttcaaagcca cgaccgaaac tttcacgcct gtgaccggtc accagaatca
    13921 tctttttatc ggggtcgata aacgggtaat ttgccgccag ttctgaacgc agcgtatcgc
    13981 tgctcatcac ctggtcacgc acccataaca gtgcatcaat aactgtatta ccagtaatga
    14041 agattcggct atccgcaacg ttttcacgca gcaagttttg ccgggaagtt tcggttggag
    14101 agaagtgata catcgccaga tgcccggtca atgtacggtt agcctcttcc ggccacggcg
    14161 aataaagatc gcccgtgcgc agaccagcct caacgtgacc aacaggaata cgctgataaa
    14221 acgccgccag gctggttgcc agcgtcgtcg ttgtgtcgcc gtgaaccagc acgacgtctg
    14281 gtttgaactc ggcaagaata ggttttagcc cttccagaat ccgacaggtt atctctgtca
    14341 ggccctgtcc tggctgcatt atgttgagat cgtagtcagg tacaatggaa aagagtttca
    14401 gcacctgatc gagcatctcc cgatgctgcg cagtgacgca aactttagcc tcaaaaaaag
    14461 gatcttttgc caacgcatgc accaacggcg ccatcttgat ggcttccggg cgcgtaccaa
    14521 atacagtcag tactttcaca tcgattctct tcgaataagc ggcgagcgcc tttgcgctca
    14581 ccgcagcagt gttgctattt cgagcaacgg cgggttaatg cgacaccagc cccgatcagc
    14641 cccccgacaa tgccccacat aatcatcagg aaggcacgac gtgggctatc gcgttttacc
    14701 ggttcttccg gcgtacgcaa atagcgatag gtctgaaaac gcggatccag ggttggacca
    14761 acattcaggg tgtttaacat ggcccgattc tgatcatagt cgagatcaaa ggccggaccg
    14821 acggcctgta aattttccag tcgagcctgg agcattggac gcccaagcag gaacatttct
    14881 gaatcaggta attcctcggc aggcacatct gtcgcactgc gcgaaatatt atgctgctca
    14941 gcaattttca gcgcctgttc aatactgttc atccggcggt cgtagatggc tttcgccacc
    15001 tcttcctgac gcttcacctg tgctttcatc tggatggtac gcgccgccca tgcgcctttc
    15061 agctcatcat tcagatggct ggctgcacgc tggctggcaa aagcaacata ctgacgtaac
    15121 aggttattag cgtcaggcgc ggtttcagca ataagcttca cgctgtcatt gaccgcgcgg
    15181 gtaaagtctc cggggataaa ctggatgttg ttaatcattt catccagcaa cgccgcatcg
    15241 gctttgctgt tgcccaccat ccgctgtttg taatagtcgg tttgcagcca gaactctctg
    15301 cgggtatccc acgaggccag ttgcataaca aactctttat aggcttcgtc catgaccgat
    15361 ggttggtcgg cagaagccat gtttgaacgg acatccaggt tacgcaaaaa ttgctgctgc
    15421 gagtaatatc cccccagcat attcaccgtt ggacgatcgg taatcgccgt cgagctccac
    15481 tcctgacgag caaaaaaagt atacgccagc gcgattaacg caaatgccag ccccatgcca
    15541 ataatccata gcttcccagc ccacaaggta cgaaacaacc cacgaatatc cagttcattt
    15601 tcagcgtctt cggccggttt cccaggcatt ggttgtgtca tcacatcctc atttatttgg
    15661 ttaaattggg gctgccacca cgatttctac gcagtctgcg ttttacgcgc ttaataaagc
    15721 gagcaacttt ccaggcacgc ttaatgcaat atccatagag gaagaatgct agcaaaaaga
    15781 gcaccagcat gacccactcc gggacaaaat gagaatattc tgccagcacg ccaatggaag
    15841 cgagcagtgc cgcagcaagg gtaatcagca caaacgcctg acgggaagta aacccggcac
    15901 gcatgatcaa atggtgaata tgctgacggt caggagagaa tgggctcatg cctttacgca
    15961 ggcgacggta cataatcgcc accatatcca ttagcggaat ggcgattatc cacaaagcgg
    16021 taaccgggct gatgggatgg gttttgccct gggtcgtttc gagcaggatc cagataacgg
    16081 taaaaccaat cagcgtactg cccgcatcac ccataaagac tttgtagcgg cgacccagga
    16141 taccaaggtt aagcatgatg tatggcagga tggcggcgat catcgcaaag caccagattg
    16201 cgaggctggt ttgcccgtcg aaccacaaaa tcataccgat tgctgcaaac gagacgcagg
    16261 acaacccgcc cagcaagcca tcaatgccat caaccatgtt gaacgcatta atggccgccc
    16321 agacggcaaa tagcgtcagg aagtaaccaa acggtccgag caccatctcc caggagccaa
    16381 agatataacc caggctactg agataaagtt tgccgaacac catcataaca atgccaacag
    16441 cggcctgtat ggtggcacgg atttttacgc tgatatcaaa acggtcatcc agcgcgccaa
    16501 tgaaaacaag cacaccggca caagcgagat agagagatgc atgcggaata tagtaatcga
    16561 caattccgaa cgtgaagcaa atccctgcgt aaaccgaaat ccccccaacg agaggtatca
    16621 atccctggtg acgtttgcgg aagtttggtt tatccactaa accgactttt tttgccacct
    16681 tacgggcaaa aaacagaaac agtgtcgtga ataaaaaaat actgatgaga tcagtactca
    16741 ctgtcagtaa attcacaatg catgctctca gagaaaatta ttagcagaag tataaccacg
    16801 aagaccttta ttcagaaggg aaagtccgtt ccgaacccac cagtcctgtg caaaaattaa
    16861 ccatttgata tgcaaattgc ataacacgcc agcaaattcc aggaattttc ctaacatcgc
    16921 cagcgcggca ttaagattac agatataaaa gcaaaacgcc acgtaaacac gtggcgtttt
    16981 tggcataaga caaatttatg agcgtttcat catttcgaag aaatcgtcat tggtcttggt
    17041 catggccagt ttattaatga ggaattccat tgcatcgatt tcgcccatcg ggtgaatgat
    17101 tttgcgcagg atccacattt tctgcagttc ttcctgagta gtgagcagtt cttctttacg
    17161 ggtaccggaa cggttgtagt cgatagccgg gaagacgcgt ttttcagcga tcttacgaga
    17221 gaggtgcagt tccatgttgc ctgtaccttt aaactcttcg tagataactt cgtccatttt
    17281 agaaccggta tcgataagcg ccgtcgcgat aatagtcagg ctgccgccct cttccacgtt
    17341 acgcgccgca ccaaagaagc gtttcggacg atgcagggcg ttggcatcca caccaccggt
    17401 caacacttta cctgacgccg gaacaacggt gttgtaagcg cgcgccagac gagtgatgga
    17461 gtcgagcaga atgataacgt ctttcttgtg ctcaaccagg cgtttggcct tctcgatcac
    17521 catttccgca acctgaacgt ggcgagatgc gggttcgtca aaggtagaag caacaacttc
    17581 accttttacc agacgctgca tctcggttac ttcttccgga cgttcgtcga tcagcagaac
    17641 catcagcaca caatccgggt ggttgtaagc aatgctctgg gcaatgttct gcagcagcat
    17701 ggttttaccg gctttcggcg gtgccacaat cagaccacgc tgaccacgac cgataggtga
    17761 tgccagatcc agtacgcgag cagttaaatc ttcagtagaa ccgttaccac gttccatacg
    17821 cagacgagaa tttgcgtgca gcggggttaa gttctcaaag aggattttgt tgcgggcgtt
    17881 ttcaggtttg tcgaagttaa cttcgttaac tttcagcagc gcaaaatagc gttcaccttc
    17941 tttcggcggg cgaatcttac cagagatggt atcaccagtg cggaggttga aacggcggat
    18001 ttggctaggg gaaacgtaga tgtcatcagg accggcgagg taggagctgt ctgcggaacg
    18061 gaggaaacca aatccatcct gcaatatctc cagtacgcca tcaccaaaga tatcttcgcc
    18121 actctttgcg tgctgcttca ggatggcaaa aataatgtcc tgcttacgca tgcgagccag
    18181 gttttccagc cccatatttt cgccgagagt gatcagctca gaaaccggcg tattctttaa
    18241 ttcggtaaga ttcatagtgg tgtgagttct taaacttggg gtaaatctcg aacttaatgt
    18301 tgtgaatggt atggcaggat catccatgcc tgtttacggt catcgactca tgtcttttcg
    18361 ctgcctggtc acaggagagt acgcagaact gaaacgacaa gacggattga gtgacgagcc
    18421 agaaatctgt tcacttcgca tttaagataa aacgggaagc gttggaaatt acaagattca
    18481 aacttaataa ggtatgttta atacgaagtc aacactaagt tagcatgact cacgccgggc
    18541 gtccagtttt tagcgacggg gcacccgaac atgaaattcc cttacgccag gttagcgtcg
    18601 aggaactctt tcaactgacc tttagacagt gcgcccactt tggttgccgc cacttcaccg
    18661 tttttgaaca gcagcagagt cgggatacca cggatgccat atttcggcgc agtgccaggg
    18721 ttttgatcga tgttcagttt tgcaacggtc agtttgccct gatattcgtc agcgatttca
    18781 tccagaatcg gggcgatcat tttgcacgga ccgcaccact ctgcccagaa atcgacgagg
    18841 atcgccccgt ccgctttgag tacatccgtg tcaaaactgt cgtcagtcag gtgaataatt
    18901 ttatcgctca tatataactc cacaggaata agcctggcgt gttggtttcg ttgttggtgt
    18961 aacattaacc aactaaaggt tgactttatt tcaccggata cgctttcgta aagcaatagt
    19021 aagctgatat tctaccacac tatgagcaaa acacatttaa cagaacagaa gttttccgac
    19081 ttcgccctgc atccgaaggt tgtagaagcc cttgaaaaaa aagggtttca taactgtacg
    19141 cccattcagg cactggccct tccgctgacg ctggcgggtc gtgacgtagc cgggcaggcg
    19201 caaaccggta ccgggaaaac gatggcgttt cttacgtcaa cgtttcatta tcttctctct
    19261 catcctgcga ttgccgatcg caaggtgaat caaccgcgtg ccttaattat ggcaccgacg
    19321 cgtgaacttg ccgtgcagat ccatgccgac gcagaaccgc tggcggaagc tactggcctg
    19381 aagctgggtc tggcttacgg tggtgatggc tacgacaaac agctgaaagt gctggaaagc
    19441 ggcgttgaca ttctgattgg caccacgggt cgtttaattg actacgccaa gcagaaccac
    19501 attaacctcg gtgccattca ggtagtggtt ctggatgaag ccgatcgcat gtacgatctg
    19561 ggctttatta aagatatccg ctggctgttc cgccgtatgc cgcctgcaaa ccagcgcctc
    19621 aacatgctgt tctccgccac gctttcgtac cgggtacgtg aactggcgtt cgagcagatg
    19681 aacaatgccg aatatattga agtggaaccg gaacagaaaa cgggccaccg tattaaagaa
    19741 gagcttttct acccttctaa cgaagaaaaa atgcgtttgc tgcaaacgct gatcgaagaa
    19801 gagtggccag acagagcgat tattttcgcc aataccaaac accgttgtga agagatctgg
    19861 ggccacctgg cggcagatgg tcatcgtgtc ggtttgttga caggcgatgt cgcgcagaaa
    19921 aaacgtctgc gtattcttga tgaatttacc cgtggcgatc tggatattct ggttgccacc
    19981 gacgttgccg cgcgtggttt gcatattccg gcagtgacgc acgtctttaa ctacgattta
    20041 cccgatgact gtgaagatta cgttcaccgt attggtcgta caggtcgcgc aggcgcaagc
    20101 ggtcactcta tcagcctggc gtgtgaagag tatgcattga atttgcctgc tattgaaacc
    20161 tatattggtc actcaattcc ggtaagcaaa tacaatccgg acgcattgat gaccgatctg
    20221 ccaaaaccgc tgcgcctcac gcgcccacgt acaggcaatg gtccgcgtcg tactggcgct
    20281 ccgcgtaatc gtcgtcgttc aggttaaata aaatgtcata ctcaaaatca ttcaaactgt
    20341 atcaagatgg caagagaatg aatcccgatg agcatactgt agtaagtgat tcaggtgaaa
    20401 gaatgcagcc aacacagaga cagattgaag gatgaagagt atgggttcca cctcgtcgct
    20461 gtatgcagcc attgatctcg gttcgaatag ttttcatatg ctggttgtgc gcgaggtggc
    20521 tggaagcatc cagacgctga cgcgaataaa acgcaaagtg cgtctggctg ctggcctgaa
    20581 tagcgaaaat gccctgtcta atgaagcaat ggagcgcggt tggcaatgtc tgcgcctgtt
    20641 tgctgaacgt ctgcaagata tccctccctc gcaaattcgc gttgtcgcta cggcgacatt
    20701 acgtcttgcc gtcaatgcgg gtgattttat tgccaatgcg caggaaatcc tcggttgtcc
    20761 ggtacaggtg atcagcggtg aagaggaagc acgtctgatt tatcagggcg ttgctcacac
    20821 aactggcggt gccgatcagc gcctggtggt ggatataggc ggtgccagta ctgaactggt
    20881 aaccggcacg ggtgcacaaa ccacctcgtt gttcagcctg tcgatgggct gcgtcacctg
    20941 gctggaacgc tattttgccg atcgtaatct ggggcaggaa aattttgatg ctgcagaaaa
    21001 agcggcacgc gaagtgttac gtccggttgc cgatgaatta cggtatcacg gctggaaagt
    21061 gtgcgttggt gcttccggca ccgtgcaggc gttacaggaa atcatgatgg cacaggggat
    21121 ggatgaacgc attaccctgg aaaagttgca gcaattgaaa cagcgagcca ttcattgcgg
    21181 tcggctggag gaactggaga ttgacgggct gacgctggaa cgtgcgttag tgttcccgag
    21241 tggtctggcg atcctgatcg ccatttttac cgaactgaat attcagtgta tgaccctggc
    21301 gggcggtgcg ctgcgtgaag gcctggtcta cggcatgttg catctggccg tcgagcagga
    21361 tattcgcagc cgtacgctgc gtaatattca gcgccgcttt atgatcgata ttgatcaggc
    21421 gcagcgcgta gccaaagttg cggctaactt cttcgatcag gtggaaaagg aatggcatct
    21481 tgaagcaata agccgcgatt tgctcatcag cgcctgtcag cttcatgaaa tcggcctgag
    21541 cgttgacttt aaacaagcgc cgcaacacgc tgcttatctg gtgcgtaatc tggatcttcc
    21601 cggttttacc cccgcacaga aaaaattgct ggcgacgcta ctgctcaacc agactaatcc
    21661 ggtcgatctc tcatcgctgc atcagcaaaa tgccgtaccg ccgcgcgtcg cagaacaact
    21721 ctgccgttta ctccgcctgg ccatcatttt tgccagccgt cgccgtgacg atctcgtgcc
    21781 agagatgaca ttacaggcta accatgaact gttgaccttg acgcttccgc aaggttggct
    21841 aacccaacat ccgctgggta aagagattat tgctcaggaa agccagtggc agagctatgt
    21901 ccactggccg ctggaagtgc attaatgagt aagtgccgga tgcgatgctg acgcatcttt
    21961 tccggccttg attatttccc tcgttttgcc gccatcatcg ctttcagatt cgccagatgg
    22021 ctttgccctt tctgcatccg ttcttcggcg ctgaccactt tgcgctcctg ttcccaaatc
    22081 agatcatcct gcggcagctc cagcaaaaag cggctcggct ccgggcgcac cagctcgccg
    22141 tactgacggc gttctttaca cagcgtaaag gtcaattcct tctgggcgcg ggtaatgccg
    22201 acataggcca gccgccgctc ctcatcgata ttatcttcat cgatgctgct ctggtgcggc
    22261 aaaaaccctt cttccatacc gaccatgtag acataaggaa actccagccc ttttgacgcg
    22321 tgcagagtca tcagttgcac ctgatccagc tcttcttcac tctcaccacg ctccatcatg
    22381 tcgcgcaaag taaagcgcgt caccacctgg gtgagcgtca tcggctcatc cagttcgctg
    22441 ccttccagca tctccgtcat ccagctaaac agttggttga cgttcttcat gcgcatttcg
    22501 gcggctttcg ggctgggcga tgtttcgtac agccaggatt cataatccat gccgtggatc
    22561 agatcgcgca ccgcggcaat cggctcccgc tccgccagac gctggatttc tgccaaccag
    22621 tgagtaaagc gggtcaatgc ttcataacca cgtccgctaa gcgtctgact caggcccata
    22681 tcaaagctgg cggtaaacat gcttttattg cgcgtcatcg cccactcacc cagctttttc
    22741 agcgtcgccg ggccaatctc tctcttcggc gtgttaacga tacgcagaaa tgcgctgtca
    22801 tcgtccgggt tagtcagcac gcgcagataa gccagcaagt ccttgatttc aggacgagag
    22861 aaaaacgacg taccaccaga aattttgtac gggatgcggt tttgcatcag gaatttttca
    22921 aacactcgcg actgatggtt accgcgataa agaatagcgt aatctttgta ctgcgtttta
    22981 ttgacgaagt gatgcgcaat cagttcacca gtgacgcgct cggcttcatg ttcttcgtta
    23041 ttcgcgctta aaactttaag ctctgtgccg taacccagtt cggagaacag acgcttctca
    23101 aagacgtgcg ggttattggc gatcaggatg ttcgccgctt tcaaaatacg tccggaagag
    23161 cggtagttct gctcaagctt aatcaccttc agcgccggaa aatcctgact cagcagcacc
    23221 aggttttgtg gacgtgcgcc gcgccaggag tagatcgact ggtcgtcatc gcccaccacg
    23281 gtaaagcgcg cgcggctgcc caccagcagt ttcaccagtt catactggct ggtgttggtg
    23341 tcctgatact catccaccag cagatagcga attttgttct gccagcgctc gcggacttct
    23401 tcattgcgtt gcagcagcaa cgtcggcaat aaaatcagat catcgaagtc gagaacattg
    23461 cacgctttca ggtgtgcatc atacagccca taacaatggg caaaaatacg gtcccgctcg
    23521 ccaatcgcac tggctgccgc ctgggacggt gttttgagat cgttcttcca gttagagatg
    23581 gtcgaaatca actgttgcag gagaactttg tcatcttcaa tcagcccttc ggtcaactct
    23641 ttgagcaacg ccagctgatc ggtatcgtca aacagcgaaa agttcgcctt catccccagc
    23701 gccgcatact cacgcttgat gatatccagc cccagcgtgt ggaaggttga gatcatcagc
    23761 ccacgcgcct ctttgcgacc cagcgtctgt ccgacacgct ctttcatctc gcgcgccgcc
    23821 ttattggtaa aggtcaccgc cgcgatatgt cgcgcctgat aaccgcaacc gcggatcaga
    23881 tgggcgattt tattggtgat cacacgagtt ttaccggaac ccgcgcccgc cagcaccagg
    23941 caggggccgg taacgaattc gacagcttgt tgttggccgg ggtttagacg cataggtgta
    24001 ttgctcaatc ttcgaacggg gggaggattg tagcagaatc gcatttgaat gtgaccgcgt
    24061 gtaagccgct aaccgattgg ctgcggatgt ttaaatcaga gagggaagac cgaaaccgga
    24121 gttcatcgtg aatccattag acttagaaaa tatcgggtcg taacgttgtg ttattacttt
    24181 ttcagagttt actaaggaga ggtgtactcg tggcgattaa tttcagtcct aaggttggtg
    24241 aaatactgga ttgcaacttt ggtaactatc ctgttagcca gaatggacag ttcagcacca
    24301 cgtattacga tggccgcatt cctcctgaaa tgataaaaaa caggttagt
//
LOCUS       DAZPDU010000052        23923 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZPDU010000052 DAZPDU010000000
VERSION     DAZPDU010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23923
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.052"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..3881)
                     /gene="entF"
                     /locus_tag="ACHURS_001882"
     CDS             complement(<1..3881)
                     /gene="entF"
                     /locus_tag="ACHURS_001882"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFR1237629.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFEQPEIIDLRTNIDPHGTAQALML
                     TDLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNMGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRATLNR"
     gene            complement(3878..4096)
                     /gene="ybdZ"
                     /locus_tag="ACHURS_001883"
     CDS             complement(3878..4096)
                     /gene="ybdZ"
                     /locus_tag="ACHURS_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFR1237630.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCLLSAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(4099..5301)
                     /gene="fes"
                     /locus_tag="ACHURS_001884"
     CDS             complement(4099..5301)
                     /gene="fes"
                     /locus_tag="ACHURS_001884"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFR1237631.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQV
                     PEIAAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRDGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5544..7784
                     /gene="fepA"
                     /locus_tag="ACHURS_001885"
     CDS             5544..7784
                     /gene="fepA"
                     /locus_tag="ACHURS_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFR1237632.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDSYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYQQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSEMVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            7959..8579
                     /gene="entD"
                     /locus_tag="ACHURS_001886"
     CDS             7959..8579
                     /gene="entD"
                     /locus_tag="ACHURS_001886"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFR1237633.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSVQTARELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASER
                     QTEAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(8701..8853)
                     /gene="hokE"
                     /locus_tag="ACHURS_001887"
     CDS             complement(8701..8853)
                     /gene="hokE"
                     /locus_tag="ACHURS_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFR1237634.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(9204..9356)
                     /gene="hokE"
                     /locus_tag="ACHURS_001888"
     CDS             complement(9204..9356)
                     /gene="hokE"
                     /locus_tag="ACHURS_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFR1237635.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            9325..9593
                     /locus_tag="ACHURS_001889"
                     /pseudo
     CDS             9325..9593
                     /locus_tag="ACHURS_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9796..10914
                     /locus_tag="ACHURS_001890"
     CDS             9796..10914
                     /locus_tag="ACHURS_001890"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFR1237636.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWADD"
     gene            10980..11228
                     /locus_tag="ACHURS_001891"
                     /pseudo
     CDS             10980..11228
                     /locus_tag="ACHURS_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
     gene            11293..11661
                     /locus_tag="ACHURS_001892"
     CDS             11293..11661
                     /locus_tag="ACHURS_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFR1237637.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            11755..12408
                     /gene="nfsB"
                     /locus_tag="ACHURS_001893"
     CDS             11755..12408
                     /gene="nfsB"
                     /locus_tag="ACHURS_001893"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFR1237638.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            12516..13763
                     /gene="ybdG"
                     /locus_tag="ACHURS_001894"
     CDS             12516..13763
                     /gene="ybdG"
                     /locus_tag="ACHURS_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFR1237639.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(13831..15207)
                     /gene="pheP"
                     /locus_tag="ACHURS_001895"
     CDS             complement(13831..15207)
                     /gene="pheP"
                     /locus_tag="ACHURS_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFR1237640.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPDKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(15309..18452)
                     /gene="cusA"
                     /locus_tag="ACHURS_001896"
     CDS             complement(15309..18452)
                     /gene="cusA"
                     /locus_tag="ACHURS_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFR1237641.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIASVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPSVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(18464..19687)
                     /gene="cusB"
                     /locus_tag="ACHURS_001897"
     CDS             complement(18464..19687)
                     /gene="cusB"
                     /locus_tag="ACHURS_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFR1237642.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALHSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATNAH"
     gene            complement(19703..20035)
                     /gene="cusF"
                     /locus_tag="ACHURS_001898"
     CDS             complement(19703..20035)
                     /gene="cusF"
                     /locus_tag="ACHURS_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFR1237643.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEVQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(20059..21441)
                     /gene="cusC"
                     /locus_tag="ACHURS_001899"
     CDS             complement(20059..21441)
                     /gene="cusC"
                     /locus_tag="ACHURS_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFR1237644.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRTAMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            21598..22281
                     /gene="cusR"
                     /locus_tag="ACHURS_001900"
     CDS             21598..22281
                     /gene="cusR"
                     /locus_tag="ACHURS_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFR1237645.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            22271..23719
                     /gene="cusS"
                     /locus_tag="ACHURS_001901"
     CDS             22271..23719
                     /gene="cusS"
                     /locus_tag="ACHURS_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFR1237646.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDAQGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTEPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
BASE COUNT         5786 a         6512 c         6055 g         5570 t
ORIGIN      
        1 tacctgttta gcgttgcgcg aataatcggc ccaattttta caaacgcccc tggagagata
       61 atatccacat gcgcacaatc ctgacgatag atatccagct ccgcaatcca cggcgaccag
      121 gcgcgttcag gactcatacc ttcctgaagc gtgcgttcag caacaaacaa cgtcgcttta
      181 ccgtcaaacg gtacgctatg agcagtcgtc agcaggcgca cagcatcagc gtagttgcct
      241 tcaatggtgg taaacaactc cgttgaagta cttccctgct gtgccgccag gaaggcctcg
      301 cgctcgcggt taatctccgc cagcacttcc gggtccagac cattagcttc tttttcctgc
      361 cagttttgcg tttctggcgg ccaggtatcc agcaagccaa gaaatgccac ctgttcgcca
      421 cgggcacgca gtcgcgccgc aataccctgc gccagcgtac cgccaaggga ataccccagc
      481 aggtaataag ggccgtgcgg ttgttgctca agtaacgttg ccagatgcgc ttcgcagact
      541 tcatccaggt ttgtcgctgt ctgcatgggt ccatgagggc gcggcgactg aatgccaata
      601 atcgaccatt gtggatcgag ataacgcgag agtacgctaa actgccaggc aaaaccggac
      661 gccggatgga aacaaaacag cgtcgggcca ttaccttcac gcaacggcag aatggtttcg
      721 aatcccatgc gccgggagct gtcctcttca ccatcaataa tcgttgccag tttggcgacg
      781 gttgacgcga ccatcacctg ccccggcgtc acctgacgag caaactgtcg acttaactgc
      841 gctgccagtt tcattgccag tagcgaatga ccgcccagcg caaagaaatc agcatcggca
      901 tcctgcacgt cacaacccag caacgacgcg aatgccgcgg cgataatcgt ttcactgccc
      961 gctttcggtg cacgtcccga cgcttgtgtc ttcagttcag gcaacggtaa ggctttgcga
     1021 tccagcttgc cgttggcgct aagaggtaac tgtggaagtt gcagcagaac caccggtacc
     1081 atatgtggtg gcaatgtttc acgaagctgc gcctgcaatg cgctggtatc caacggcagg
     1141 cccgattgcg acaccagata gcccaccaac tgacgcgcat caccaccagt ggctgccgcc
     1201 tggttaatca cacaggcgtg ggtaacggct tgttcgacat ccggcagcgc ctgcatcacg
     1261 cgatcgattt cgcccagttc gatacgctgc ccgcgaattt ttagctgatc gtcactgcgc
     1321 ccgaggtact ccaccgcgcc gttatccagc cagcgggcaa cgtctccggt acggtacatc
     1381 cgttcgccag gagcaaaagg atcggcaata aagcggctgg cggtcagatc ggggcgtccg
     1441 agatagccct gcgccagttg aatgccggta agatagagat cacccgccac acccggcggc
     1501 accggatgca tcatcgcatc aagaatacgc agccccatgt tccacaccgg ataaccaatc
     1561 ggcacactgc tgccgcgcac ctgtgccagt tcctcgccaa aagccggata ccagctgaca
     1621 tctaccgccg cttccgtcgg gccatacaga ttatgtaacg gcgcaccggt taactgttgc
     1681 cattcacggc ataaatcagc cggtaaggct tcaccactac agaaaacctg tttcaacgtc
     1741 gcgcaactct ggcgagcggt ttgcggagtc agcgaggcaa caaatgccgc cagcatcgac
     1801 ggcacaaagt gcgtggtcgt tacgccatat tcggcaaaga attgctgcat agcgagcggg
     1861 tcacggtgcg cttccggttc agccatcacc agttttgccc ctgcgataaa cggccagaaa
     1921 aactcccaca ccgagacatc aaaactgcac ggcgtttttt gggcaacgac atcttcgcct
     1981 gtaagcgggt agtgattttg catccataac aggcggttaa cgatagccgt ctgcccgacc
     2041 attacccctt tcggcctgcc ggtggagcca gaggtaaaga tgatataagc cgtgtggtgc
     2101 ggttgtgaaa gttgcagcgg cgcactgccc tggggggtaa gcggggcgtt atagcaaagg
     2161 cttgttaaat tgggaacatc gctaaagcgc ggcagttgat cgtcggtggt aattaacagc
     2221 gacggacgcg catcttccag catcattttc aggcgatcgt ccggatagcc ggtatccagc
     2281 ggtagccagg ccgcgccagc ttcaactatt gcatggagtg ccagggtcaa aaagaccgag
     2341 cgcggtagtg ccaccgccac gctgtcccct ggtttaacgc cgcgctcacg cagcagattc
     2401 gccagcgcca ccacctgctc gcgcatttcc cgatagctga acaggtaacg cgcatctgcc
     2461 agcgccggag catccggtgt ttttgccgct tgttctgcca ccagcgcgct cagcgtggtt
     2521 tctggaatct caacctgagt ggcgttgatc tgcgccagct gcgcatactc acctggcagc
     2581 ataatatcga catcgccgca caacagcgcc ggatccgcgg cgaactgggc aatcagcatt
     2641 ttcaggcgtt cagcatgctg gattaacgtt ggctcatcgt aacgctgttt attggcgagg
     2701 atctcaatac tcaaatcacc gtgttcatcc gggaacaggg ccagttcaag gtcattaacc
     2761 ggaccggttg ccagggtatg ggtttgcgcc tgaacaccag gaatatccag ttggtaatca
     2821 aataccttaa tattgagtac cggaccaaac agcggttcat cacctgccgc tcgcccgctg
     2881 tcacggacaa tttgttcggc atcgtaacgt tgatggcgac gcattttttt cagttgagcc
     2941 gccagtcggg ttgccagttc cggcagcgtt tcttgcgccg caatgtgaat acccaacggt
     3001 aaaacgttga gcacgggtcc gctagccgtc agcgccgccg agcccagtcg acgcataaag
     3061 ataaatccgg cggcatagtc catgcgattg cacaatcgcc ccagccacaa ggctgccagc
     3121 gcaagggcta aatcggtacg ctgcacacct gaaagttgcg tagccagctg gcggaattcc
     3181 ccgtcggtaa attccagttt caggcgcaga atatctgccg aagcgctgcg ccccggtaaa
     3241 ggtgccggag aaagtgacgc gggcggcggc agttgacgac gctgttctgc ccagaatgcc
     3301 gcatcacgct gccaggcttc gctttcgcgg tattgctggt actcttccac tacatcagcg
     3361 aaaggcgtaa acggcgaagc aggcgttggt tcgccacgca gccatgtgca gtaaatattg
     3421 gcgatctggc gagtaatggc cgggaaactg aagccatcga ccagcaaatg gtgataacgc
     3481 tgataccagt accagcggtt atccgcaaca tgtatcagct gatgaaagac cagtggttta
     3541 ccgctatcga cgcgcaaatc ttgttgcaaa tccgtcagca ttaatgcctg cgcagtaccg
     3601 tgcggatcaa tattggttcg caggtcgata atttctggct gttcgaatat cagcgcatca
     3661 tcgacccact gccagacttc gccgttatct tccgtaaaac gcatccgcaa cgtatctgct
     3721 tgcgctagtc cggcaaccac cgcgcgggcc agtaatggcg catcaacatc tccagttaac
     3781 tcaacgtaat gcgccacgct ccaggcggag ggtaaatccg acagtttttc tgccatccag
     3841 atgccgggct gtgcggcgac caaaggtaaa tgctggctca ttgtgcctcc tgcaactggg
     3901 tgaaattggc cggtgtcaga gtacgccagt ggacttccag ccactgctgg caggacgcct
     3961 gtgactgcgg ctgacacaca atgtcccagc ctgccgataa gaggcattgt tgcggccaca
     4021 gactgaattg cccctgcgca ttgcgcaaaa tgtaaaacgc tccctgcgga tcatcgaagg
     4081 gattactgaa tgccatactc aactcctgtc atggaaaagt ggttgccaga ggtcgattaa
     4141 cccctgcatc aagccaccgc gccaacaaag cgcatcatgt ccgccgtcaa cctgacgcca
     4201 gaaaatggat tcttttatag ggtgcaattg cgcatacagc gcctgattgg cccgcatgat
     4261 catcggctcg cgaatacccg cttccagcac aatgcgcaga ccttcggcgc taacttcacc
     4321 agcttttagc ttttcaagta acacgccctc ttgctgcccg tcccgatgcg gccaccagta
     4381 cgatcctgac tggcttaata cacagccaaa gcgttcaggc cagtgcagtc cggcatacag
     4441 cgcggacagc ccaccaaaac tctgcccggc gaccacggtg cgatcggcgc gatcgctaaa
     4501 aggggcaata gctttcacca ggggtaataa ctcttgctgt actgccagcc agaaatccgc
     4561 attacacggc agttcgtggg cgcggtgcgt ggtgtcgata gcgtcgatca acacatacac
     4621 ggcgggagga agttgctgac gatgggtcag cgaagtcagc acaggccaga cgggcatact
     4681 ttgcgcccaa aattcgccat cgagcaaaac tgccagcgga cgttcttctg ctgttacatc
     4741 gccggtggta aaaatccata cacgacgtga atttttcaac cgttcacttt tccagataat
     4801 ttctttggct gctatttctg gcacttgcgg acaatcccat cccggttgca gaggcgcttg
     4861 cggcatttcg agtgcagaaa cagcgtgccc tcgcccgcct ttccagcttt gtggattcag
     4921 cggatcggct attgcctggg gtaatagttt tcgccagcct tcacgtaatt cgaggcgatc
     4981 gggggatggt gcagaaaaaa tgtcatcgcg ttcggtggga ataaagcagt agctgccgcg
     5041 ccagttggca ttgagttgtg tcgtccactg ccagacatca gtacctgcaa ttcgctgcat
     5101 cgactggggc tggctgttct gatgatgatc ggtcacacca gtgatgtaga tccatacgcg
     5161 ctttatcgtc gagtattctt cagaaccttg gggatcacgc caccagaaag tgacctcaaa
     5221 catttcgtca ttcagacgct gccattccgg gccatgtttc gactgccacc agctctcact
     5281 tcctactttt aacgccgtca caccataacc ccatgtttac tgtgcaattt ttcattgatt
     5341 gcagaaatat attgataata ttattgataa ccatttgcat ttgcaatagc gtaatggcgc
     5401 gccgtgggaa gcgcggacat ttattaacca actgcactgc gtgtctttca ggatcaaagg
     5461 ttttcgcggt agcgggatgc gtcgtgttga ggacgaccat gcccgacagt tgcaattcgt
     5521 ggcaaaaatg caggaataaa acaatgaaca agaagattca ttccctggcc ttgttggtca
     5581 atctggggat ttatggggta gcgcaggcac aagagccgac cgatactcct gtttcacatg
     5641 acgataccat tgtcgttacc gccgccgagc agaacttaca ggcgcctggc gtttcgacca
     5701 tcaccgcaga tgaaatccgc aaaaaccccg ttgcccgcga tgtgtcggag atcatccgta
     5761 ccatgccagg cgttaacctg accggtaact ccaccagtgg tcagcgtggg aataaccgac
     5821 agattgatat tcgcggtatg ggtccggaaa acacgctgat tttgattgac ggcaagccgg
     5881 taagcagccg taactcggtg cgtcagggct ggcgtggcga gcgcgatacc cgtggtgata
     5941 cctcctgggt gccacctgaa atgattgaac gtattgaagt tctgcgtggt ccggcagctg
     6001 cgcgttatgg caacggcgcg gcgggcggcg tggttaacat cattaccaaa aaaggcagcg
     6061 gtgagtggca cggctcctgg gacgcttatt tcaatgcacc agaacataaa gaggaaggtg
     6121 ccactaaacg caccaacttc agcctgaccg gtccgctggg cgacgaattc agcttccgct
     6181 tgtatggcaa cctcgacaaa acccaggctg acgcgtggga tatcaaccag ggccatcagt
     6241 ccgcgcgtgc cggaacgtat gccacgacgt taccagccgg gcgcgaaggg gtgatcaaca
     6301 aagatattaa tggcgtggtg cgctgggatt tcgcgccatt gcaatcgctg gaactggaag
     6361 caggttacag ccgccagggc aacctgtatg cgggcgacac ccagaacacc aactccgatt
     6421 cctatacccg ctcgaaatat ggcgatgaaa ccaaccgtct gtatcgccag aactactcgc
     6481 tgacctggaa cggcggctgg gataacggag tgaccaccag caactgggtg cagtatgaac
     6541 acacccgtaa ctcgcgtatt ccggaaggtc tggcaggcgg taccgaaggg aaatttaacg
     6601 aaaaagcgac acaggatttc gtcgatatcg atcttgatga cgtgatgctg cacagcgaag
     6661 ttaacctgcc gattgatttc ctcgttaacc aaacgctgac gctgggtacg gagtggaatc
     6721 agcaacggat gaaggacttg agttccaata cccaggcgct gaccggggcg aacaccggcg
     6781 gcgctattga tggtgtgagt gccaccgacc gtagcccgta ttcaaaagca gaaattttct
     6841 cgctgtttgc cgaaaacaac atggagctga ctgacagcac catcgtaacg ccggggctgc
     6901 gtttcgatca tcacagtatt gtcggcaata actggagccc ggcgctgaac atatcgcaag
     6961 gtttaggcga tgacttcacg ctcaaaatgg gcattgcccg tgcttataaa gcaccgagcc
     7021 tgtaccagac taacccgaac tacattctct acagtaaagg tcagggctgc tatgccagcg
     7081 cgggcggctg ctatcagcaa ggtaacgatg acctgaaagc agaaaccagc atcaacaaag
     7141 agattggtct ggagttcaaa cgcgacggct ggctggcggg cgtcacctgg ttccgtaacg
     7201 attatcgcaa taagattgaa gcgggctatg tggctgtagg gcaaaacgca gtcggcaccg
     7261 atctctatca gtgggataac gtgccgaaag cagtggttga aggtctggaa ggatcgttaa
     7321 acgtaccagt tagcgaaatg gtgatgtgga ccaataacat cacttatatg ctgaagagtg
     7381 aaaacaaaac cacgggcgac cgtttgtcga tcatcccgga gtacacgttg aactcaacgc
     7441 tgagctggca ggcgcgggaa gatttgtcga tgcaaacgac cttcacctgg tacggtaagc
     7501 agcagccgaa aaaatacaac tacaaaggtc agccagcggt tggcccggaa accaaagaaa
     7561 tcagtcctta cagcattgtg ggtctgagcg cgacctggga tgtgacgaag aatgtcagtc
     7621 tgaccggcgg cgtggacaac ctgttcgaca aacgtttgtg gcgtgcgggt aatgcccaga
     7681 ccacgggcga tctggcaggg gccaactata tcgcgggtgc cggggcgtat acctataacg
     7741 agccgggacg tacgtggtac atgagcgtaa acactcactt ctgatgctaa cgtcagattg
     7801 ttgacaaagt gcgcgtcgtt catgccggat gcggcgtgaa cgccttatcc ggcctacaaa
     7861 aatcttgcca attcaatata ttgcaggacc atgtaggcct gataagcgta gcgcatcagg
     7921 cagttttgcg tttgtcatca gtctcgaata tggtcgatat gaaaactacg catacctccc
     7981 tcccctttgc cggacatacg ctgcattttg ttgagttcga tccggcgaat ttttgtgagc
     8041 aggatttact ctggctgccg cactacgcac aattgcaaca cgctggacgt aaacgtaaaa
     8101 cagagcattt agccggacgg atcgctgctg tttatgcctt acgggaatat ggctataaat
     8161 gtgtgcccgc aatcggcgag ctacgccaac ctgtctggcc tgcggaggta tacggcagta
     8221 ttagccactg tgggactacg gcattagccg tggtatctcg tcaaccgatt ggcattgata
     8281 tagaagaaat tttttctgta caaaccgcaa gagaattgac agacaatatt attacaccag
     8341 cagaacacga gcgactcgcg gactgtggtt tagccttttc tctggcgctg acactggcat
     8401 tttccgccaa agagagcgca tttaaggcat cggagagaca aactgaagca ggttttctgg
     8461 attatcagat aattagctgg aataaacagc aggtcatcat tcatcgtgag aatgagatgt
     8521 ttgctgtgca ctggcagata aaagaaaaaa tagtcataac tctgtgccaa cacgattaat
     8581 tgacaacatc tggtacgatt cgcccgcagc catcactgac tgcgggcgaa agtgtaaagc
     8641 aggtgcctta ccactctgac ctgacaaccg gatatgcagg gattgctccc cgcacaacgg
     8701 ctacttcttc ggttcgtaag cgagaacagc cttaaactca atattacgtt cctttacggt
     8761 gaactcacac agcgagtctc cgaccagaag cgtaaatccc agcaccgtca aacacagcac
     8821 tatgactgcc acaagggcat atttcgtcag catgtttata ttgcctccat gcgaagaagg
     8881 gactaccatc cgagttgttg aggttcggag tggcagcccc aggattgata gaaatatctc
     8941 ctggggcttc atctttccag acctcagagt aaacctgaaa ccggaaagct tcaggcaccc
     9001 gccgttatct tacccttcct cagcaaaata tctgtttatt tatacagtaa gtcctgtttc
     9061 ggacttctgt tggcaacact cgttattgct cgtaaaacga aatcgcccgc agccatcact
     9121 gaccgcgggc gaaagtgtaa agcaggtgcc ctaccatcct gacctgacaa ccggatatgc
     9181 ggggattact ccccgcacaa cggctacttc ttcggttcgt aagcgagaac agccttaaac
     9241 tcaatattac gttccttcac cgtaaactca cacagcgagt ctccgaccag aagcgtaaat
     9301 cccagcaccg tcaaacacag cactatgact gccgcaaggg catatttcgt cagcatgttt
     9361 atattgcctc catgcgaaga agggactacc atccaaattg ttcgggtttg gagtggcagc
     9421 cccagggttg atagaaatat ctcctggggc ttcatctttc cagacctcag agtaaacctg
     9481 aaaccggaaa gcttcaggca cccgccgtta tcttaccctt cctcaacaaa atggctgttt
     9541 atttatacag taaatcctgt ttcggacttc tcactcctca tttaatcaac tgaagctgag
     9601 cccgtttttt ctgcatgcct caatcgtcaa aattcttcgc catcagccag ctctgccata
     9661 acttgccaca ttgaaaaagt agccaaattg cttcagtcac catcatcaaa aaaagaaaca
     9721 ttccagaact tccgcatctc gcccagccgc tccgacttct atactgaata gaaaacgcca
     9781 acataagaga aacctatgcc attacccgat tttcatgttt ctgaaccttt taccctcggt
     9841 attgaactgg aaatgcaggt ggttaatccg ccgggctatg acttaagcca ggactcttca
     9901 atgctgattg acgcggttaa aaataagatc acggccggag aggtaaagca cgatatcacc
     9961 gaaagtatgc tggagctggc gacggatgtt tgccgtgata tcaaccaggc tgccgggcaa
    10021 ttttcagcga tgcagaaagt cgtattgcag gcagccgcag accatcatct ggaaatttgc
    10081 ggcggtggca cacacccgtt tcagaaatgg cagcgtcagg aggtatgcga taacgagcgc
    10141 tatcaacgaa cgctggaaaa ctttggctat ctcatccagc aggcgaccgt ttttggtcag
    10201 catgtccatg ttggctgcgc cagtggcgat gacgccattt atttgctgca cggcttgtca
    10261 cggtttgtgc cgcactttat cgccctttcc gccgcgtcgc catatatgca aggaacggat
    10321 acgcgttttg cctcctcacg accgaatatt ttttccgcct ttcccgataa tggcccgatg
    10381 ccgtgggtca gtaactggca acaatttgaa gccctgtttc gctgtttgag ttacaccacg
    10441 atgatcgaca gcattaaaga tctgcactgg gatattcgcc ccagtcctca ttttggcacg
    10501 gtggaggttc gggtgatgga taccccgtta acccttagcc acgcggtaaa tatggcggga
    10561 ttaattcagg ctaccgccca ctggttactg acagaacgcc cgttcaaaca tcaggagaaa
    10621 gattacctgc tgtataaatt caaccgtttc caggcctgtc gctatgggct ggaaggcgtc
    10681 attaccgatc cgcacactgg cgatcgtcga ccgctaacgg aagacacctt gcgattgctg
    10741 gaaaaaatcg ccccttccgc acataaaatt ggtgcatcga gcgcgattga ggccctgcat
    10801 cgccaggtcg tcagcggtct gaatgaagcg cagctgatgc gcgatttcgt cgccgatggt
    10861 ggctcgctga ttgggctggt gaaaaagcat tgtgagatct gggccgatga ctaagtaggg
    10921 aattgcctta cagaagaata tccctgacaa tggtgtttta acttacattt aacaatgtta
    10981 tgaaacaccc tttagaaacc ttgactaccg cagcaggcat tttgctgatg gctttcctct
    11041 cttgcctgct gctgcccgcc cccgcactgg ggctgacgct ggcacaaaaa ctggtgacca
    11101 tgttccatct gatggatctt agctagcttt acactttatt gttttgtctg tggtttttag
    11161 tgctgggcgc tattgagtat tttgttctgc gctttatctg gcgacgctgg ttctcgctgg
    11221 cggattaaac gtcgattatc gccgtacttc gcggcatact ttgcttattc tccttcgcct
    11281 aaaggaacgt ttatggataa gcaatcactg cacgaaacgg cgaaacgcct ggcccttgag
    11341 ttaccctttg tcgagctttg ctggcctttt ggcccggagt tcgatgtttt taaaattggc
    11401 ggcaaaattt ttatgctgtc gtcggagctg cgcggcgtcc cctttatcaa tctgaagtcc
    11461 gatccacaaa aatccctgtt aaatcagcag atatacccga gcattaagcc agggtatcac
    11521 atgaataaaa agcactggat ttcggtgtat cccggcgagg aaatctccga agcgttactt
    11581 cgcgatctga tcaacgattc gtggaatctg gtggttgatg gtctggctaa acgcgatcaa
    11641 aaaagagtgc gtccaggcta aagcggaaat ctatagcgca tttttctcgc ttaccatttc
    11701 tcgttgaacc ttgtaatctg ctggcacgca aaattacttt cacatggagt ctttatggat
    11761 atcatttctg tcgccttaaa gcgtcattcc actaaggcat ttgatgccag taaaaaactt
    11821 accccggaac aggccgagca gatcaaaact ctcctgcaat acagcccatc cagcaccaac
    11881 tcccagccgt ggcattttat tgttgccagc acggaagaag gtaaagcgcg tgttgccaaa
    11941 tccgctgccg gtaattatgt gttcaacgaa cgtaaaatac ttgatgcctc gcacgtcgtg
    12001 gtgttctgtg caaaaaccgc gatggacgat gcctggctga agctggttgt tgaccaggaa
    12061 gatgctgatg gccgctttgc cacgccggaa gcgaaagccg cgaacgataa aggtcgcaag
    12121 ttcttcgctg atatgcaccg taaagatctg catgatgatg cagagtggat ggcaaaacag
    12181 gtttatctca acgtcggtaa cttcctgctc ggcgtggcgg ctctgggtct ggacgcggta
    12241 cccatcgaag gttttgacgc cgccatcctc gatgcagaat ttggtctgaa agagaaaggc
    12301 tacaccagtc tggtggttgt tccggtaggt catcacagcg ttgaagattt taacgctacg
    12361 ctgccgaaat ctcgtctgcc gcaaaacatc accttaaccg aagtgtaatt ctctcttgcc
    12421 gggcatctgc ccggctattt cccctcagat tctcctgatt tgcataaccc tgtttcagcc
    12481 gtcatcatag gctgctgttg tataaaggag acgttatgca ggatttaata tcccaggttg
    12541 aagatttagc gggtattgag atcgatcaca ccacctcgat ggtgatgatt ttcggtatta
    12601 tttttctgac cgccgtcgtg gtgcatatta ttttgcattg ggtggtactg cggaccttcg
    12661 aaaaacgtgc catcgccagt tcacggcttt ggttgcaaat cataacccag aataaactct
    12721 tccaccgttt agcttttacc ctgcagggga ttatcgtcaa tattcaggcg gtattctggc
    12781 tgcaaaaagg caccgaagcg gcagatattc tgactacctg cgcgcagttg tggatcatga
    12841 tgtatgcgct gctttcagtc ttctcgttgc tggatgttat tttgaatctg gcgcagaaat
    12901 tcccggcggc atctcagtta ccgctgaaag ggatatttca ggggattaaa ctgatcggcg
    12961 cgatcctggt cggcattttg atgatctcgc tgctgattgg tcagtcgcca gcgattctga
    13021 tcagcggtct tggtgcaatg gctgccgtgc tgatgttggt atttaaagat ccgattcttg
    13081 gtctggtggc cggtattcag ctttccgcga acgatatgct gaaactgggc gactggctgg
    13141 agatgccgaa atacggcgca gatggcgcgg tgatcgatat tgggttaacc accgtcaaag
    13201 tgcgtaactg ggacaatacc attaccacta ttcccacctg gtctctggtt tctgactcct
    13261 ttaaaaactg gagcgggatg tcagcatctg gcgggcgacg tattaagcgc agtatcagta
    13321 ttgatgtcac cagtattcgt tttcttgatg aagacgaaat gcaacgtctg aataaagcgc
    13381 atttgttaaa gccttattta accagccgcc atcaggaaat taatgagtgg aatcgccagc
    13441 aaggttctac ggagtcggta ttaaacctgc gccgaatgac caatattgga acctttcgtg
    13501 cctatctgaa cgaatatctg cgtaaccatc cgcggattcg taaagatatg accttaatgg
    13561 tacgccaact ggctccaggt gataacggtt taccgctcga gatctacgcg tttactaaca
    13621 ccgtggtgtg gctggaatat gaaagcattc aggctgatat attcgatcac atatttgcga
    13681 ttgtcgaaga gtttggtctg cgacttcatc agtcgccaac cggcaatgat attcgctctc
    13741 tggcgggtgc atttaagcag taattaaaaa aaccgctctc atcgaatgga tgagagcgga
    13801 catcgttttg tcggatgtag cgtgaatgcc ttatttccga cgcagcgttt taaatgccac
    13861 aaacaggaat acaatccaca ccggcagcag gatcgctgac aagcgcatat catccatcgt
    13921 gcacatcagc agcaaaatca tgccgaggaa ggcaatgcag agatagtttc cgaacggata
    13981 gagcagcgcc ttaaactgtg tttcacgccc ctgacgtcgc atcgctgcac gaaaacgcag
    14041 atgcgccaga cagatcataa tccagttcaa cagcagcgtt gctaccacca gcgccatcag
    14101 cagaccaaac gctttttgcg gcagcagata gttgattaac accaccagcg aagtgatcgc
    14161 tccggaaagc atcagcgagt taatcggcac accgcgacgg ctgacgcgag tcaaaaactt
    14221 cggcgcatta ccctgcacag aaaggccaaa cagcatgcgg ctgttagagt aaaccccgct
    14281 gttatacact gacagcgatg ctaccagaat gacgaagttc agcgcagaag ctaccacgtt
    14341 gctgtcgaga ttatggaaaa tcatcacaaa cgggctactg ttggatttca cttccaccca
    14401 cggatagagc gccagtaaaa ccaccagtga accgatgtaa aacagcagga tgcgatacac
    14461 cacctgattt accgcttttg gaatgctttt atccggatcg cgcgcttcag cggcagtaat
    14521 cccaatcagc tccagaccgc cgaaggagaa cataattacc gccagcgaca aaatcagccc
    14581 attccagccg gtggcgaaga aaccaccgta gcgccagagg ttgtcgatac tggctttctc
    14641 gccgccgtga ccagaaaaca gcagccacag gccaaagccg atcataccga tgattgccag
    14701 cactttaatc aacgcaaacc agaactcggt ttcgccatat aagcgcacgt tcaccaggtt
    14761 aacggcgttg atgataataa agaaggcggc agcccaaatc cacgttggaa catccgggaa
    14821 ccagtactgc atatagatgc ccgcagcggt cagctctgcc attcccacca gcacgaacat
    14881 tacccagtag ttccagccag agaggaagcc cgcaaacggt ccccagtatt tataggcaaa
    14941 gtgggcaaat gaaccggata ccggctcttc gaccaccatc tcgccaagct ggcgcataat
    15001 caggaaagcg atgatcccgg cgacgccgta gcccagcaat acagccggac ccgccatctg
    15061 aatcgccgga ccaatgccaa gaaacagacc agtaccaatt gcgccaccca gcgcaatcag
    15121 ttgaatatga cggttatgta atccgcgatg aagcgtcggc tcttgattcg acgcagtatc
    15181 ttccgatacg gttgacgcgt ttttcacgcc tttcccctgt gtgtcttttt tgttgagggg
    15241 caccttttaa catttagtgc ccatcgtcgc aagacacaat ccacacggtt aaacggggta
    15301 tcctgctttt atttccgtac ccgatgtcgg tgcagccaca tcagcttata cgccgccggg
    15361 ataataaaca gcgacagcaa aggtgcggtg atcatgccgc caatcatcgg tgcggcaatc
    15421 cggctcatca cctctgaacc agcccccgtt ccccacagaa tcggcagcag accggcgata
    15481 atcaccgcca ccgtcatcgc tttcgggcgc acgcgcagta ccgcgccgtg atacaacgcc
    15541 tcatccagct tctgctcact gaatgtttgc ggattattca acgacggctc ggcctctatg
    15601 gcgtgacgta aatacatcag catcaccacg ccaaattcgg cggcgacccc ggcaagggca
    15661 ataaaaccgg tgcccgttgc cacagaaaga tgaaagccca tccaccacag caaccagatg
    15721 ccgcccacca gcgcaaacgg tacgctgctg ataatcagca acgcttcgcc cacccgacgg
    15781 aatgccagat acaacagcac gaagatgatc atcagtgtca tcggcaccat gagtttcagc
    15841 ttatggttgg cgcgctccag tagctcgaac tgcccggaga atgccacgct ggtgcccggt
    15901 tttaactgca ctttttcagc tatcgctttt tgcaaatcgt gaaccaccga caccatgtca
    15961 cgatcgcggg catcgatata aatccagctc gtcgggcgcg cattctcggt tttcagcatc
    16021 gacggtccgg tagagacttt aacgtcggcc acgtctgcca gggtgatttg ctgcttcatc
    16081 ggcgtcagga tcggtagctg gcgcagtgcc tgcgggctat cgcgccagct ttgcggataa
    16141 cgcagattaa tgggataacg ggcaatccct tccaccgttt cgccaaccat cgccccgccc
    16201 accgcagaag tcacaaacaa ctgcacatcc gccaccgtca taccgtaacg cgcggctttt
    16261 tcacggttaa tctcaacgtt gatatagcgc ccaccttcca gacgctcggc tagagcagaa
    16321 gccacgcttg gcaccgttcg cgctacttct tcaatttgct cagccatcgc gtcgatatcc
    16381 gccagcacag tgccggaaac tttaatgccg atggggcttt taatgccggt tgagagcata
    16441 tcgatacggt tacgaattgg cggcacccac agattcgcca gccccggcag ccgcacggtg
    16501 ttatccagtt cctcaatgat tttgtccatc gtcatgccag gccgccactg atcctgcggc
    16561 ttaagctgga tggtcgtttc aaccatctca agcggagcag aatcggtggc ggtttccgct
    16621 ttcccggttt tgccaaatac ccgcgccact tcaggtacgc tcataattag cttgtcggtt
    16681 ttttgcagca tactcgccgc ctctgctgcg gaaatccccg gcagcgtcga tggcatatac
    16741 aacaagtcgc cttcattgat ctgcggtaaa aattccccgc caactttatt gagcggccag
    16801 agaaccgtca gcaccgaaag cgctgccacc aacagcgtgg ttttcggcca gtgcagtact
    16861 ttcagcaata gaggatgata aacacgaatc aaaaagcgat tgagcgggtt actgctttcc
    16921 ggcggaattt tgccacggat ccagtacccc atcaggatag ggatcactac gatcgccagc
    16981 agcgccgcgc ccgccatcgc ataggtttta gtgaacgcca acgggccaaa cagacgccct
    17041 tcctgccctt ccagggtgaa gatcgggata aacgacaacg tgataatcag cagactgata
    17101 aatagcgccg gacccacttc aacagatgca tcggtgatca cctgccagcg cgttttatta
    17161 tccagcgtgg cgtcaggatg ctggtgctgc cactcttcca gccgtttatg tgcattttcg
    17221 atcatgacga tagcggcatc gaccatcgcc ccgacggcaa tcgcaatgcc gcccaacgac
    17281 ataatattgg cattcagtcc ctggaagtgc atgacaataa aagcaataca caaccccagc
    17341 ggcaacgaaa taatcgccac cagcgccgag cgcacatgcc agagaaacag cgcacagact
    17401 accgccacca cgataaactc ttccagcaac ttgccgctga ggttgtcgat ggcgcggtca
    17461 atgagctggc tgcgatcgta tgttgtaact atctccacgc cttccggcag actacttttc
    17521 agcgtttcca gtttgtcctt cacggaggcg atcacttctc gggcgttttt gccggagcgc
    17581 aggatcacca ccccgcccgc cacttcgcct tcgccattca gttcggcaat gccccggcgc
    17641 atctccgggc caatctggac cttcgcaaca tcacgcagat aaacgggcac gccattttca
    17701 ctggctttta aaacgatgtg attaaagtcg tcgagcgttt gcagatagcc gctggcgcgc
    17761 accatatatt ccgcttccgc cagttcgatc gacgaaccgc ccgcttcctg gtttgaagca
    17821 tccagcgcgc tttttacttc ggctagactg atgccatatt gcgccaggcg ctggggatcg
    17881 ataaccacct gatactcttt caccacgccg cccaccgacg ccacttccgc aacgtcaggg
    17941 atggttttta gctcatattt gagaaaccag tcctgcaatg agcgtaaatc ggccagatcg
    18001 tgcttaccgc tgcgatccac cagtgcatat tcatagatcc agccaacacc cgtggcatct
    18061 ggtcccagct cggcgctgac tcccgcaggc agcttaccct gtacctggtt gaggtactcc
    18121 agcacccgcg aacgcgccca gtacggatcg gtaccatctt cgaaaatgac atacacataa
    18181 gagtctccaa actgcgagaa accgcgcaca gtcttcgcgc caggcaccga caacatggtg
    18241 gtggttagcg gataagtcac ctgattttca acgatttgcg gtgcctgacc gggatagctg
    18301 gttttaataa tcacctgcac atcggagaga tccggcagcg catccactgg cgtgttaatg
    18361 atggtccagg tgccccagat gctcagaaac aacgcgccca tcagcaccaa aaaacggttc
    18421 gccaccgagc gacgaataat ccattcaatc attggttatt ccctcaatgc gcattggtag
    18481 cactttcaga gcgcatccgc tccagtgcgc cagaaatatt ggcttcggaa tcaatcagga
    18541 acaggccgct ggaaaccacc ttttcacctt ccgccagacc agagtgtaac gcggtgacgc
    18601 cttgcgatgc ctggaaaacg gcaacgcgtt tcggtacaaa gcgtccgtcg gcatcaacgg
    18661 taatcacccg ctgttcgttg ccggtatcaa tcagcgcctg tgacggaatg agcagcatcg
    18721 gttcgctggc ggtgttgagt tgcaaccagg cgttcatgcc cggttttagc gcctcgtcgg
    18781 cgttgtcgac ttccagacgc agctgcagcg tgcgggtcgc ggcatccacg ccaggtagca
    18841 gcgtccattt gcggatggtg agtgttttat ccggtcgcgc cggaacggtg agcgtaaact
    18901 gcgaggcatc tttcaccagc caggcgatag actccgggat cgcagcagtg acccacaccg
    18961 ggtccatacc ctgaattttc gctaccacgt tatctttggc gatattcatt cccgcgcgca
    19021 gatcaaacgc ggtgatcacg ccatcaatgg gtgctttgag cgtaaagcga gtctggattt
    19081 tttgcgtggc gatcaggcgg cgaatatccg cctccggcat tcccgccagt cgcagccgct
    19141 caagaatgcc ttcagtctgg gtcgccgtac cgccggtttc gcgcagcagt aaatactcac
    19201 tctgcgcttc cacccagtca ggaatggtca ggtcgagaag cggtgtgccc ttttgtactt
    19261 tatcgcccac ggtaagcgga tacaccttgt cgataaaccc ggcagcgcga gcctgcacaa
    19321 tggcatactg atactcgttg taactgacat tcgccgggaa actctgggca aaagtcagcg
    19381 gtccgcgcgt gacggtagcc gttttcaccc ccagattctg cgtctgagtc gggtcaatgc
    19441 gcacaccaga cgcagaactc tcttcatcgg catatttcgg caccagatcc atatccataa
    19501 acggcgattt acctggttta tcgaaccgcg tattggggta catcgggtcg taccagaata
    19561 agattttacg ttctgcggtc gacgtttttt ctgcgggtgg ttcagccttt gcaaaccagg
    19621 taaaacctgc cgcagaaata ataccgcccg cgatcatgct gccgataata agcgcgattt
    19681 ttttcatctc attaaacctg ggttactggc tgactttaat atcctgtaat aaagaaaggt
    19741 tgccctgctg gacaaaatta aacgccactt tgtcgccggt tttaatttca ctcattttcg
    19801 tctgcggggt gatggtaaag cgcatagtca tctccggcca gttcacggca gcaatcggat
    19861 cgtgatggat ggtgattttt ttgctttcca gatcaatacc ctttaccacg ccagtggcgc
    19921 taataacctg cggttgtact tcgctcatgg tttcatgatg atgttcgtta gcctgggcat
    19981 taaagccaat aacggtaaac agactgaaca ttgcgacttg cagtgctttt ttcatttatt
    20041 ctctcctgga gttaaaaatt attgctgcca accgccccct aaggcggtat acaaagaaat
    20101 ttcgttaacc tgacgggcat aattcaggtc gagtaaagtt tgtcgggttg caaataaaga
    20161 acgctcggca tccagcactt ccagataact taccgcgccg tgctgatata atgcccgcgc
    20221 ccgttgcaaa gtaatttgca gcgacgccag ataacgctgc tgggcgctga tttgatcgtt
    20281 caggctttga cgtaatgcca gcgcatctgc cacttcttta aaggcgttct ggattttctg
    20341 ttcataattc actaccgact gctgctggcg aatttcggcg atatccagat tggcctggtt
    20401 gcgtccggca ttaaaaatgg ggatctcaat tttaggaata aaattccaca tcccgctgct
    20461 agcgttaaat aatgaagaca gatcgctgct ggcggttgat attccgctgg tcaggctgat
    20521 agacgggaaa aatgccgcac gcgcagcacc aatattagca ttagccgcca ttaacgcgtg
    20581 ttcagcttcc ataatatccg ggcgctgcaa taagatttgc gacgacaagc ccgccggtaa
    20641 tttaacgctt tgcaggctgc cgctgtttac tgtctgcgct tgcggcagtt tgccgtagct
    20701 tcccaataac agttgcaatg cattattcgc ctgcgccagt tccccctgac gtttagcgat
    20761 gtcactgcgg gtactttcta tcaccccgcg agcctgttcc agcgccagaa cattgctgct
    20821 accggtcaac agttgttttt cgacaaacgc atatgactgc tgataattac gcagcgtttc
    20881 ttcggctatt tgcaattgcg catacgccag ttgctgattg aaatagcttt gcgcgacatt
    20941 agaaaccagc agaatatgca ccgcgcgctg agcttcctca gtggctaaat aattttgtcg
    21001 ctcggcttcg ctcatgttct ttaagcgacc gaaaaaatcg aggtcaaagc tggcgttaag
    21061 gccggtcgaa aactcccgtg tcgtggcggt attcccttta agattaccgc tccagctgcc
    21121 gctgccctcg ccattgagct gtgggtagcg gtcggcatcg gtcagacgat attgcgcccg
    21181 tgcctcctgc actttcagcg tcgccatgcg caaatcccgg ttattcacca gcgcctcgct
    21241 aatcagcgtc ttcacctgat tatcaacaaa aaaggtgcgc cagcccacgt tctggtagtt
    21301 atctgctgcg ttaaccaagc cgttctggct gagtgagaac tgctgcggca cgggcattgc
    21361 cgtacgctga taatccggtg ccaatgaaca accggttagc gcaagggcca cacaaaatgg
    21421 cagaagttta caaggagaca taggctcata atttctggtg attttatacc gccaacttta
    21481 ctcgccaggc tctgattttc cggtgacagg aaaatgacaa aattgtcatt ttgccaataa
    21541 gcgattgcca tctgatcccg ctactctaga attgcccggg caacatgcgg aggaaaaatg
    21601 aaactgttga ttgtcgaaga tgaaaagaaa accggagaat acttgaccaa agggttaacc
    21661 gaagccggtt ttgtggtcga tttggccgac aacgggctga atggctacca tctggcgatg
    21721 accggtgatt atgacctgat aatcctcgat attatgctgc cggacgtgaa cggctgggat
    21781 atcgtgcgca tgttgcgctc cgccaataaa gggatgccga ttctgttgct taccgcgctt
    21841 ggcaccattg aacatcgcgt caaggggctg gagttggggg ccgatgacta tctggtgaag
    21901 ccgttcgctt ttgctgaact gctggcgcgg gtgcgcactc tcctgcggcg cggggcggcg
    21961 gtgattatcg aaagtcagtt tcaggttgcc gatctgatgg tcgatctcgt cagccgcaaa
    22021 gtcacccgca gcggcacgcg catcactctg accagtaaag agtttactct gctggagttt
    22081 tttctccgcc atcagggcga agtgctgccc cgctcgctta tcgcctcgca ggtgtgggac
    22141 atgaatttcg acagcgacac taacgccatt gatgtggcgg tgaagcggct acgcggcaaa
    22201 atcgacaacg actttgagcc gaagctgatt cagaccgtgc gcggcgtggg ctatatgctt
    22261 gaggtgccgg atggtcagta agccatttca gcgcccgttt tcgctggcaa cccgtctgac
    22321 cttttttatc agcctggcca ccatcgcggc gtttttcgcc tttgcatgga tcatgatcca
    22381 ctcagtgaaa gtgcattttg ccgagcagga tattaatgat ttaaaagaaa ttagcgccac
    22441 ccttgaacgg gtacttaatc atcctgacga gacgcaagcc cgacgcttaa tgacgctgga
    22501 agatatcgtc agtggttatt ccaacgtgtt gatttccctg gcagatagcc acggcaaaac
    22561 ggtgtatcac tcccccggtg cgccggatat ccgcgagttt acgcgtgacg ccatacccga
    22621 taaagacgct cagggtggcg aggtgtatct cctttccggc ccgacgatta tgatgccagg
    22681 ccacggtcac gggcatatgg aacacagcaa ctggcggatg attaacttgc cggttggccc
    22741 gttggtggac ggcaaaccga tttatacgct ctacatcgcg ctttcgatcg attttcatct
    22801 tcattacata aatgatttga tgaataaact tattatgacc gcatctgtca tcagcatcct
    22861 gatcgtcttt atcgtgctat tggcggtaca taaaggtcac gcgccgatcc gcagcgtcag
    22921 ccgtcaaatc cagaatatca cctcgaaaga tctcgacgtt cgccttgacc cgcagaccga
    22981 gcccattgag ctggaacagc tggtactgtc gttcaaccat atgatcgagc gtattgagga
    23041 tgtctttacc cgccagtcca atttctcagc ggatatcgct cacgaaattc gcacgccgat
    23101 taccaatctc atcacgcaaa cggaaatcgc cctcagccag tctcgcagcc agaaggagct
    23161 ggaagatgtg ctctactcta atctcgaaga gctgacgcga atggcgaaaa tggtcagcga
    23221 tatgctgttt ctcgctcagg ccgataacaa ccagctaatc cccgaaaaga aaatgctcaa
    23281 cctggcggat gaagtcggca aagtgttcga ttttttcgag gcgttagcgg aagatcgcgg
    23341 cgtggagttg cgatttgttg gcgacaaatg tcaggttgcg ggcgatccgc tgatgctgcg
    23401 tcgggcgtta agcaacctgc tttctaatgc cctgcgttat acgccaacca gagagacaat
    23461 tgtagtgcgc tgtcagacgg tcgatcacca ggtgcaagtt accgtcgaaa accccggtac
    23521 gcccattgcg cccgagcact taccgcgatt gtttgaccgt ttctaccgcg ttgacccttc
    23581 ccgccagcga aaaggcgaag gtagcggcat tgggctggcg atagtgaaat cgattgttgt
    23641 cgcgcataaa ggcacggttg cagtaacgtc agatgcgcgg gggacaaggt ttgtgattat
    23701 gctgccggag agagagtgat tattaagttc aaattcaggg cgtggtgcat cacctcctgg
    23761 tattgttgat caacaaatta gagaaaaact tattaaacat atgaaatatc gaaggctttt
    23821 atttttcaaa gtttcacgag atgtttgtat atgaaactta gttaaatctg tgtttcttcc
    23881 aattgtagat cacaatgccc tttctaaatt ttcatcgtta taa
//
LOCUS       DAZPDU010000053        23440 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZPDU010000053 DAZPDU010000000
VERSION     DAZPDU010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23440
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.053"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..674)
                     /gene="focB"
                     /locus_tag="ACHURS_001902"
     CDS             complement(<1..674)
                     /gene="focB"
                     /locus_tag="ACHURS_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HFR1237647.1"
                     /translation="MRNKLSFDLQLSARKAAIAERITAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRH"
     gene            complement(696..2708)
                     /gene="hyfR"
                     /locus_tag="ACHURS_001903"
     CDS             complement(696..2708)
                     /gene="hyfR"
                     /locus_tag="ACHURS_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HFR1237648.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGCRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSHLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGLSVREVL"
     gene            complement(2738..3151)
                     /gene="hyfJ"
                     /locus_tag="ACHURS_001904"
     CDS             complement(2738..3151)
                     /gene="hyfJ"
                     /locus_tag="ACHURS_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFR1237649.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFPCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(3144..3902)
                     /gene="hyfI"
                     /locus_tag="ACHURS_001905"
     CDS             complement(3144..3902)
                     /gene="hyfI"
                     /locus_tag="ACHURS_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HFR1237650.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKQHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVISSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(3899..4438)
                     /gene="hyfH"
                     /locus_tag="ACHURS_001906"
     CDS             complement(3899..4438)
                     /gene="hyfH"
                     /locus_tag="ACHURS_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFR1237651.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFELTVTNKA
                     DLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPECKQ
                     RATLLNDDTDVPLVAKEQL"
     gene            complement(4448..6163)
                     /gene="hyfG"
                     /locus_tag="ACHURS_001907"
     CDS             complement(4448..6163)
                     /gene="hyfG"
                     /locus_tag="ACHURS_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HFR1237652.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDTETYPFINEGNSDARVIPIGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(6153..7733)
                     /gene="hyfF"
                     /locus_tag="ACHURS_001908"
     CDS             complement(6153..7733)
                     /gene="hyfF"
                     /locus_tag="ACHURS_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFR1237653.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHILNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(7738..8388)
                     /gene="hyfE"
                     /locus_tag="ACHURS_001909"
     CDS             complement(7738..8388)
                     /gene="hyfE"
                     /locus_tag="ACHURS_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFR1237654.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(8400..9838)
                     /gene="hyfD"
                     /locus_tag="ACHURS_001910"
                     /pseudo
     CDS             complement(8400..9838)
                     /gene="hyfD"
                     /locus_tag="ACHURS_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416979.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
     gene            complement(9855..10802)
                     /gene="hyfC"
                     /locus_tag="ACHURS_001911"
     CDS             complement(9855..10802)
                     /gene="hyfC"
                     /locus_tag="ACHURS_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFR1237655.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEDAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(10813..12831)
                     /gene="hyfB"
                     /locus_tag="ACHURS_001912"
     CDS             complement(10813..12831)
                     /gene="hyfB"
                     /locus_tag="ACHURS_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HFR1237656.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFEFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNIFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVVCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTRVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(12831..13448)
                     /gene="hyfA"
                     /locus_tag="ACHURS_001913"
     CDS             complement(12831..13448)
                     /gene="hyfA"
                     /locus_tag="ACHURS_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFR1237657.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(13701..14171)
                     /gene="bcp"
                     /locus_tag="ACHURS_001914"
     CDS             complement(13701..14171)
                     /gene="bcp"
                     /locus_tag="ACHURS_001914"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFR1237658.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(14171..14743)
                     /locus_tag="ACHURS_001915"
     CDS             complement(14171..14743)
                     /locus_tag="ACHURS_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFR1237659.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            14889..15767
                     /gene="dapA"
                     /locus_tag="ACHURS_001916"
     CDS             14889..15767
                     /gene="dapA"
                     /locus_tag="ACHURS_001916"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFR1237660.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            15784..16818
                     /gene="bamC"
                     /locus_tag="ACHURS_001917"
     CDS             15784..16818
                     /gene="bamC"
                     /locus_tag="ACHURS_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFR1237661.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            17031..17744
                     /gene="purC"
                     /locus_tag="ACHURS_001918"
     CDS             17031..17744
                     /gene="purC"
                     /locus_tag="ACHURS_001918"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFR1237662.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMLNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            17912..18760
                     /locus_tag="ACHURS_001919"
     CDS             17912..18760
                     /locus_tag="ACHURS_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFR1237663.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHNMQ
                     QQGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGDPAQCN
                     TFGKSI"
     gene            18775..20790
                     /gene="tmcA"
                     /locus_tag="ACHURS_001920"
     CDS             18775..20790
                     /gene="tmcA"
                     /locus_tag="ACHURS_001920"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFR1237664.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSDPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRGSRIAVHPARQREGVGQQLIASALQYRPGLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNNAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNTSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            20864..21562
                     /gene="ypfH"
                     /locus_tag="ACHURS_001921"
     CDS             20864..21562
                     /gene="ypfH"
                     /locus_tag="ACHURS_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR1237665.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIEMVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(21672..21872)
                     /locus_tag="ACHURS_001922"
     CDS             complement(21672..21872)
                     /locus_tag="ACHURS_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFR1237666.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(21900..23027)
                     /gene="dapE"
                     /locus_tag="ACHURS_001923"
     CDS             complement(21900..23027)
                     /gene="dapE"
                     /locus_tag="ACHURS_001923"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFR1237667.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIDRLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(23031..23387)
                     /locus_tag="ACHURS_001924"
     CDS             complement(23031..23387)
                     /locus_tag="ACHURS_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFR1237668.1"
                     /translation="MVTLYGIKNCDTVKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
BASE COUNT         5868 a         6228 c         6275 g         5069 t
ORIGIN      
        1 aaatggcgaa tggcaatggc gaacggaatc acaaacaaat tggcgataca gtgctcaaac
       61 ccactggcga caaacagggt gatgggcaaa atcatggcga cgattttgtc gcataatgaa
      121 cgcccgcaat aactcatcca cagcgccaga caaaccatca gattgcacat aatgccgagg
      181 ctgacagatt cagtaaatgt atgatgcatt ttgccctcgg cgcagtgtaa aaccgcgact
      241 ccccacatcg cgttttcact catcaccagc ccggaaaacc agattaacaa actgaaacag
      301 gcaatacctg ccagattacc gcaggccacc agaagtgcgt taatcagcca agttcgccaa
      361 ctaataacgc cccgactttt tgccatcacc gtcattaccg acgaggtgaa caggctggtg
      421 ccacaaaccg ccagcaaaat aaagccgagt gtaaagcaaa ggccgcccac cagatgggtt
      481 aatgcctgtg aagacggggc atcggcgata acggaaaggt aaaaagtaaa tccaatcgcc
      541 ataaacacgc cagcggacat cgccattaaa aagaccgaca ctttgctgcg ggcaatttta
      601 tgggcagtaa tccgttcagc gattgccgct tttctggcgc tcaactgcaa gtcgaaagag
      661 agtttgtttc tcatgcatac tcctgcaaaa gcagattaca acacctcgcg aaccgagagc
      721 cccagacgct gcattcgtga cagcagcgtg gtgcgcttca tccctaatcg tgtcgccgcg
      781 ccacggggcc cggcaacaat gccattggtt tctcgcaata cctgaacaat gcgctgacgt
      841 tcttcttcgt cattctctgg cgtcgtcggg tgcagcaact gcgccattga actgcgaagc
      901 gctgaatctt catttagcgt cggcagtaaa tggctttgtc ggacattaag atgtaaattc
      961 agactgttac cgcgagtcag cagtaccgct cgctcaatca cgttttccag ctcgcgcacg
     1021 ttgcccggcc agtcccacga catcaactgg cgtagcgcct cggtcgggat ggcgtcaatt
     1081 gaacgattca tatggcgcgc cattttttgc gtgaaatgct ttgctaaaag agggatatct
     1141 tccggacggt cgcgtagcgg cggcaattcc agtgggaaga cattcaggcg ataaaagaga
     1201 tcgctgcgaa actggcgatc ttcaaccatt tgccacaaat cacggttggt ggcggcaatg
     1261 actctgacat tcaccgggat cgttctactc ccgccgagac gctcaatctc ccgctcctgc
     1321 aatacgcgca gcagtttagg ctgaagttct aacggcagat cgccaatttc atcaagaaac
     1381 aacgtgccgc catcggcaat ttcaaaacga ccacgatggg tattaatcgc accagtaaac
     1441 gcccctttgt catgaccgaa taactcactt tccagaagac tggcggggat ggcagcgcag
     1501 ttgattttga ccagcggctt gtcgcgtcgc gggctaagtt gatggatcgc tctggcgatc
     1561 acctctttgc cggttccggt ttcaccacaa atcaacactg tactgtcgct cttcgccaca
     1621 atatctacct gctggagcag gtcttccatt gcctggcttt gatagataat gtcaccgacg
     1681 cccagattcg aaaggagctg ctcgctaagc tggtggtttt cttgctgcaa actttcctgc
     1741 aaatcggtca tgctacgcca ggcatcggca ttgccaacgg caatagcgat gcgatcggct
     1801 atgtgttgta gtagctgaca gttttcctca ctaaagagag tggaagaggt atgcgccagc
     1861 aacaatgcac ccggtgtatg gttgccaaag gtaagcggta taaggagcgc agattcgcag
     1921 ccgttagatg caagtaagag taataacggg tcgcgttgcc agagaaacag atcgtctgca
     1981 cggtgcgtca aggtcggttg tcgggtttgt agcgtttgtg tcagcaatac actgtcgcca
     2041 ggcatattgc gtggcagaca cgcacaatgt gaggcagaaa gatcgctgca ccacaggcta
     2101 aacttctcgt tctttcgatg atcgcccagt accatactga ctgaagccag accgaaaaaa
     2161 tgatggatct cacgagcgac gtcagcgatc agatcgtcga gatcaagatg tgacagcact
     2221 gcattagtaa tatcaaccag tacccgatga tgatcgcgat ctttctccag caaacgcagc
     2281 gccacctgct gttcttcatc attgagccat gattgcagaa ccggggtgat tatccgcgcc
     2341 agccagttaa acagtgtgca atcctgggga gtaaaacggc agccgtgggt gcgaataaag
     2401 cgtagctccg ccattccact actgccgaga aggacggcgt gacgcgaata atcgaccatc
     2461 tcgccctgat actctgttgt tgccgtcaca tcgctgccat catcatggta gcggtaatag
     2521 aagttgcttt tgagaacatg cagttcaata agttgtaccg ggctgaacgg cggcggcagc
     2581 gggatgaagg cgcgtaataa agacgcctta ccgtgtttct gcgctaaccg ctccgcgagc
     2641 attccgttta ccgcttcaat tgttattcct tgcggcgggg caaacatcgc ctcgtctgac
     2701 atagccatca acagtcctta ttaatgagaa atccttctca cgcaattttt ctggccatca
     2761 gatagatggc cggttcctgc acaatttcat ccaacagatg aatgaattga atgctacgcg
     2821 cctgccacac agacgtcgcg tcatccgcca gtggcgcgaa tgcccgggtc aacagggcgg
     2881 tgtggctggc gtcaataaca atctcaccaa aagtcatcac ccccagcatc ttacgtcggg
     2941 cttgctcctc ttcgaccagt gcaagccaat cttcgtattc cgtcagtggg caggggaagg
     3001 cgacattcag acaatcaatc acgccaacgt gatgaccgat agccagcgag taatacatta
     3061 cctgagagct gtgttccggc atctcgtcgc tactggcgac aaactttttt cgcagcgtcc
     3121 agaaaacaat ttccccgcac tcttcagtca tgtaaccccc ggagtacgcg aaagagttgc
     3181 tgaacgatac tattgagacg tggatcgtcg gcatcgcgca accaggtgtt aacccgattt
     3241 cctgtaggat cgtcgcttaa atggcgcagg agccgatcgc aaatttctcg cccctgacga
     3301 tagcccgcca gccgccgcgc ttctcgctca atggcgatac gctgtgatgg cgggatctgc
     3361 ggccacaacg gttgcatagt caccccggtg ggatcgcgat aatccaccgc gtgaatcttc
     3421 tgttgcagca aaccgagcgc cacggcgaaa ccgtgaatgg tggcggccgg tgttggcggg
     3481 cagccgggga tccaaacatc aatggggaca atggtgtcgc tgccgcccca gacgctgtag
     3541 agatcgtgga aaataccgcc gccgacaccg cacgcgccgt aggaaacaca aattttatga
     3601 tcgggggcag actcatacgc ccgaagtgca ggcatacgca tcgcccgggt gactgcgcct
     3661 gtaaataaca aaatatcggc gtgacgcggt gatgaaataa ccttaatgcc aaaacgttct
     3721 gcgtcgaata ctggtgtgat ggcggcaaaa atttcgattt cacaggcgtt gcagccgccg
     3781 caatcgacgc gataaacgta agccgagcga cggatatcct gtagcaaatg ctgcttcatc
     3841 ttttgcgttt gctcgtccag cgtgatgggc tggctgacat gttgtgtaag cactggactc
     3901 atagctgctc cttagccacc agcggtacat ctgtatcgtc gttgagcagc gtcgcgcgtt
     3961 gtttgcactc cgggcagacg ctcgcttgcg cccataacat ttcgcggttt tgtggcgcat
     4021 tttgttgctg tgccaacaat tcagcagcca gtgcgacggt tttttgcggg gcaaacgggc
     4081 gttcgcaacg gctgcaacgt tgtagatgga acgtcgcgcg ggtatagaga tcggctttat
     4141 tggtgacggt cagttcaaag ttattggtaa gctggatggc tctggtcggg cacacttctt
     4201 cacaacgtcc gcagtaaata caacgcctca gatagagctg ccaggtgcgc gaattttgct
     4261 ggtcgtcggt ctggatagtc agcgcatttg ccggacaagc acaggcgccg caggcaatac
     4321 attggctggg catcaggtcc ggtttcccgc gaaagcctgg gctgacctcc agtggcgcga
     4381 agggatattt caccgtcgcg gttccggcgc gcataatagt tttcagtaac ttcagcatct
     4441 tctgtcctta cttcagcggc gaacggttac gatcaatgcc gtagcgttcg atctctttat
     4501 acggcacggt ttttgactgg cgcttgcgca catctaccag cgtcacacgg tcggtacagg
     4561 agtagcaggg atcaaggcta ccgataatca gcggtgcgtc agaaacggta ttgccgcgca
     4621 gcatgtaacg caacaccggc cagttggcgt aggtggcggc acggcagcgc cagcggaaca
     4681 atttttggtt atcaccgagc atgctccagt gcacgtcttc accgcgtggc gcttcaacaa
     4741 agcccagcgc gaatgcgtga ggtttatagc taaagccttc ggtcagcagt ggggtatccg
     4801 gcatgttgtc gagggcaaat tccagcattg ccagcgaatc aaacgtctct ttgacacgga
     4861 ccatcacgcg ggagaaaaca tcgccgccgg taaaggtaaa cagtgttttt ggaatattgc
     4921 cgtagtcggc gtaggggtga tcaaagcgca aatcacgggc aaaaccactg ccgcggatca
     4981 gcggccctac agggctataa tcacgggcga tttgtcggtc gagaatgcca atgccctgag
     5041 tgcgttgttc catattcggc gtggcaagca gcatctctac cagctccgac acgtcggcgc
     5101 gcatctcgcg caccagtttc agcgtttgca gacgttgctc tttgagaata tcgcggcgaa
     5161 caccaccaat cagattcaga ccgtaggttt tacgcgaccc ggtcagcaat tccgccattg
     5221 tcatcgactt ttcccgcacg cggaaaaatt gcataaagcc ggtatcaaat ccaacaaaat
     5281 ggcaggagag gccaaggttg agcaaatgac tgtgcagacg ttcgacttcc agcagaatcg
     5341 agcgaatagt atgcgctcgt tgcggcacct caatccccag tgcattttca accgagttgg
     5401 tataggccac actgtgggca aaaccgcaaa tcccacacac gcgatccgat aagaaggtca
     5461 cttcgttgta gcccatccgc gtttctgcca gtttctccat gccgcgatgg acataaaaca
     5521 ggcggtaatc agcatcgaca atttgctcgc catccacaaa caagcggaag tgacccggtt
     5581 catcggaggt gatatgcagc gggccaatag ggatcacccg cgcatcgcta ttgccctcat
     5641 tgataaacgg atacgtttcg gtatcagtcg tcggttcagg gcgcagtcga taatccatcg
     5701 catctttgcg cagcggatgc atatcttccg gccagtcatc gggcaacacc aggcgacgct
     5761 gatccggcag gccaaccgga atcagcccgt acatatcgcg aatttctcgc tcgccccaga
     5821 ccgcggcagg gacgcgcggt gtgactgacg gaaactcccg actgtcggca tcgaccagtg
     5881 ccttcaccac gatccagcat ttttcggccc cttccattga aagggcataa taaaccgcgt
     5941 aatgaccgtt aagtgtccgc tcgtcgttgc caaacaggac tggaagccag ccatcatgtt
     6001 gataataaag ataatgtaca acgtcaggca gcagattgat tttcaccgta atggtgacct
     6061 gttcaggcgt ttgtcgctct tcatccagca ccgcgccggg gaactgcgtt ttcagggcgg
     6121 cgagaatcgc ttcgccacga tttgacgatg aattaacgtt cactgtgttt ctccgaaaca
     6181 gatgcggtgc ctgaaggcaa aaaatcatgc caggtgctac gttgtgcagg caggtcgtgc
     6241 gtccctgaga ggactatagt ggaagcgccc gccaggatcc tgatgacagg ttgtggaata
     6301 tgcgttccca tcgccagcat catgaccagc agaatcacca ttggcgaggt ggtcaaccag
     6361 ccgagatcac cccggttaac ggcctgcggc ggtttcgcca ttaacacccg cgctgccatc
     6421 cgtaccaggc ccgccagcac cagcgttaac agcaataaca gcaggacgat aatcagcagg
     6481 tgattacgtg ccagtccggc ggtaatggtc ataaattcgc taagaaaaat gttgaagggc
     6541 ggcatccctg ccagcgccag cgcaccgccg ccaaacagca cggcggtaaa tggcatgatt
     6601 ttgagcatcc cacagacgac gttgagatcg cgcgtgccgt acttgagcag tacattgccg
     6661 gaaccgcaga acagcagcgt ttttgccaga ctgtggttta agatgtgcag cagcgcggca
     6721 aaaattccca gcggcccgcc aatgcctagc gccaccgcga ccagccccat gttctccacg
     6781 ctggagtacg ccagcagacg cttaatgtcc cgctgtacca gaatgaaaaa tgccgccacg
     6841 gcaaccgaca acatgccgaa gatgagcaac aaccggttgg ggaaatcgct gccgatggct
     6901 tggcaaataa tgatgtaata gcgaatcagc accaacagcg cgcagttcag caatacggcg
     6961 gagagcaggg cgctgaccgg actcggcgct tcactgtgag catccggcag ccaggcgtgc
     7021 atgggaaata gcccggtttt ggtaccaaag ccaattagca caaacacaaa ggccaacagc
     7081 attaatgttg ggtcaagcaa ggacgattgc ttaagaacct cgctccagaa tatcgccatt
     7141 tctgcctgcg gcataacgct ggcggcgttg gcgtatacca gcacggtacc gaacagacca
     7201 aaagcgacac caacagtaca aataatgatg tacttccatg ctgattccag cgatgaacgc
     7261 tgaccgtaaa tgcctaccag aaacgccgag cttaaggtgg tggcttcgat cgccgcccac
     7321 atcacaatca ggttattgct ggtaacaacc agcagcatgg tgaacaaaaa cagatggaag
     7381 aaaccgtagt aatcgcacag cgtaacgggt gaaagctcgc cgtgtgccac ttcatgacgc
     7441 atgtagccaa tcgagtaaac accggtgaga aagccaatca caccaagaat ggcgaggaac
     7501 aaaccgccca gaccatcaat atgaagccac agtcccgcag cgaatatttc tcctgcatca
     7561 gcggcagttt ggaccaccca gagtgccaga atcagcagca gtgtgatccc taagctatgt
     7621 aatacagtca ccgtacaagt cgcagagagc ccccgtttcc ggcaggcaaa acagagcagc
     7681 gaaaaaagca gcggcgtgag caggagtaaa gcgaacatca cagaataact catctcatta
     7741 tcccttcagc gcggtcaagt tgttcacgtc cagcgtgccg tgggtacgcc atatttttct
     7801 tgccagtaac accatcacaa tgacggcgaa gatggcgtcg gtagcgatac ctatttccac
     7861 cagttccggt gctcgccagg caagaagcgc cagcaccaga tgggagccgt tttccatcag
     7921 gcagtaacca aaaatttgcc gcaggatatt gcgctggctg acaatgcaca gcaggccaag
     7981 cagaaaatga cctaacgcta ccgccagcgc cggtttcagc ccggtagcca tcggtagctt
     8041 cacgggctga acgacaaatg cgcaaagcag gacaattaac gccgcgagca gtgccatcat
     8101 tgccggaccg aataacgctt tttccgggat gttctgggga atatttcgtg cagcgtaagt
     8161 catgattaac ggtaccagca gcactttggt gataaaggcg ctggcggacc agatcagcag
     8221 ttgctctgcg gcgaacaggc acgagagagt ggcgaaaata gacaccagca ccagcgactg
     8281 gcaggcgtaa aatccgcatg acaggcgata gcttttgacg ctaatcacaa acagcgatgt
     8341 cagcatcatc agtcccgcca gattatttac gatcatagaa ccggtcattc atcatctcct
     8401 tactgcaacc aggtcgcggc gattacgctg gaaatcagcg acatcacaat cagtacaatc
     8461 aacaccaggc gcattgaccc tggcagcggt gcggcatcgg cgatggcctc gctcggtttg
     8521 cccggcacga cgcgaccaaa ccagcgaata aaccaggcga aactggcgac cgattcaatc
     8581 atcagcagaa tcatggcggg cagcaggatc cagtactcca ctgacaacgc aaaaccgacg
     8641 gcaaacagcg ggaatttact gaagaagccg ttgaacggcg gcacaccggt aatcgccagc
     8701 gctgccacgc agaaacccac gcttggcaac ggcagggtgt gcaatacgcc acgcagacgc
     8761 ggcaacaagc gcgtgccgca gctgtaactc agcgcacctg ctacaaggaa aaacaggctt
     8821 ttagcgaacg cgtggttgac gatgtaggcg atgctaccct ccagcgccag ccgcgagccg
     8881 aagatggaga gcgacaagcc gaagaacatc cagccaagtt gagtgatggt cgaccaggcc
     8941 agcaaccgct tcatatcctg ctgtggcaaa tacatcagaa agccataaag aatggtgacc
     9001 agcgccatga ccatgccaac gccgccaatc acatgcggga tattgccgcc gtcgataatg
     9061 gcgcgggcaa aaatgtaaac gcccactttc accatcgatg cggcgtggag ataggcactg
     9121 atcggtgttg gcgcttccat tgcatccggt agccacgctt gcatcggtag ctgggccgat
     9181 ttcccccacg cggcaaacag gatgccgcca taaaccagat aacgtgcgtc gccgtgtaac
     9241 tcgctcatcg cgctaagcgc aaacgttccg gtctgcaaaa acagcgtggc ggcggcaaga
     9301 tacaacccca acgagccgat atgagtgata agtaacgctt ttagtgctga aagctgtgct
     9361 ttatcgctct ggtaataact gatcaacgcc caggagcagc cgcccgtaat ttcaaaaaac
     9421 aacaactgac cgagcagcgt cgaggagagt accagtcccg ccatcgcgcc gataaacacc
     9481 agcaaaaatg cgtaataacg attcgtgccg ttatgcgggt gttcgcgatt tttatccgtc
     9541 agataacccg tggagtagat cgtgaccagc aaaccgagaa acaccaccac aaacagaatc
     9601 agcgtactca tgcgatcaat gaccaagcca aacaatgcca catcccccac gttgaccaac
     9661 gtcaacgtga cggcaacttt atcggcctga taaaacgcgg agatcagcga caacatgcac
     9721 agcgtggtca gtgcggcgaa caaaaccccc attcggcggc ccgacgttgt ggcgaaaacg
     9781 aaacgaccag tgcgccgata aaaggcagca ttaacgtcgt cagagcaaga ttttccatat
     9841 tccgtcagtg ctccttacag accggttaac cagaagaccc atgcaagcgc agcaaggctg
     9901 aagccaagcc aggtcacatg gtgaatgagt aaaaaacgcc cgcgtgccag cgtgttttct
     9961 gcgattgagg ccagtacaaa aatcagcaaa accttgagca gcgtaacgac aagtgaagtc
    10021 agcaggcagg cgagagaaag ttcttgcgcg cgcccaaagg gcagaaacag ggccacaaac
    10081 agtgctgcca tcacgacctg tttcagcccc agcccccatt tcgctagcgc cagcccggca
    10141 ccggaatatt cggtcagcgg gccttcctgt aattcctgtt ctgcttcagc aacatcaaag
    10201 ggaatttttc ccatctcaat gaagcaggca aaaccacagg ccagtaacgc cagtacggtg
    10261 gttagcggcg agttccagcc cgttgccagc gtattgctga tcatctcgat atgcgtggaa
    10321 cctgctatca gcgccaatac cagcagtgag agaatgagca tgggttcgac cagaatgccg
    10381 agcgtcaact cgcgactggc accgactccc gcaaacgggc ttccggtatc cagccctgaa
    10441 agagcaaaga aaaaacgaaa cagggcaaga agatagataa gggtgatcag atcgccgccg
    10501 cccgcaaaag gggaaacggt aataaatagt ggtagggcca tcgccagcac cagcatgctg
    10561 ctgattaata cccacggcat caggcggaac atcagacctg aagatgtcgg cgcatcttcc
    10621 tggcgtttaa acagtttgtg gatatcgcga taatcctgcc agatccccgg cccgcggcgg
    10681 gagtgcatac gcgcgcgtat ctgccgggaa atacccgtaa aaagtggggt tagcatcagc
    10741 agaatcacgg cctgtgctaa cgcaaaaatg accagatatc cgtcgcaaag agtttgtctc
    10801 atggtgattt ccttagacgg caatagcgat tagcagcaca accagtgcgg cgaccacata
    10861 caggcaatag agacgaaagt cgccgctttg cagatgctgt atttctttgg ccagccgttg
    10921 ggtggcgctc acgatggggc ggatcacccg ctcatcccag aagggttctg tgctctgagc
    10981 cctggcggtg acgtgcgcaa gacctttatt tagcctcagc gtagggtcga gctgttgtcg
    11041 tagacgaaat agcgcagaaa agaccacacg cagcggctgc atcacgccat tgcctgacgg
    11101 ggccatcgca ttttcccagc cgtagccgca tgcccaggca tctcctgtgc gacgaaacgc
    11161 tgcgcggcgc gaacgacacc acagccagta aaggccaggc agcaaaggca tcgccagtag
    11221 taacagcaac agtaatgaag gagtgacccg tgtatgaaac gtgccgggta caagtgctat
    11281 tccgctggcg acagtgacgg gaggggtatt ggtaaacgca tgggcaatgt gcattatctt
    11341 cggtgccagc caactggcac taagcgcaat taatacgcag agtgccgcga gcagtagcat
    11401 ggcgacgatc atcgtatttg gcacttcctg tgcctcttca gcgtgtgtgc tgcgcggcgc
    11461 accacagaaa gtaataccgt acattttgac gaagcacact actgccagcc caccagtgac
    11521 tgccagcatt accatagcaa taggacccgc aagttgtagc gctacggctt ccacacgact
    11581 tagtgagaat agcgactgcc aggtgtacca ttcgctgata aaaccattca gcggaggaat
    11641 ggctgatatc gccaggcaac caatcaggca tgctgcggct gtccacggca tccgtttcgc
    11701 cagtgccccc attttttcca tgtcgtgggt atgcaaacgc gaaataatcg cacccgcgcc
    11761 gagaaatagc agccctttga aaagcgcatg gttgagcaga tgaaacagtg cgccgagcag
    11821 tccaaccacg gtgagcagcg ggtcgtgcag tgacagaccg accatcgcca caccgaccgc
    11881 cagcaaaata atgccgacgt tttcgacggt actccaggcc agcagccgtt tgatatcctg
    11941 ttccgccagt gcatacagca cgcccaggag cgcggagatt gcgccgatcg ccatcaccag
    12001 aatgccccac cacagcggca aacccgtttg cgccagcaga tccatcgcca ctttcaggat
    12061 gccgaaaata ccgattttga ccattacgcc agacatcaac gctgaagcgt gcgatggtgc
    12121 ggcagggtga gcgcgcggca accagctgtg caacggcatc atcccggctt tcgcgccaaa
    12181 accgaaaaag gccagcagga acaccgccga cgccagcccc ggagaaagtg aaagcgtgcg
    12241 gaaactggca aaatcgaggc tgccgctttc gcgccccatc agcaagaagg cgatcattat
    12301 cagcaccgat ccggcgtggg cgataaaaaa gtagagcatg ccagcgttga tcgacgtttt
    12361 atcctgcctg gcaatgacca gaaaccagga agacagcgac atcatttcaa acagcacgat
    12421 gaaccaaaaa gcgttgtcca tcaccagcag ggcaaccatc gatgcgatga aaatattcat
    12481 aaagaagccc atcgccgccg cgcctttgcc ctcgtattcg cgcatataag tcaatgaata
    12541 gagagaacaa accaccacca gcaaggagat aaccagcacc ataaatgccg agaggctatc
    12601 cattcgcagg gttaaatcgg caaattcaaa aggcgtggca aaacgggcga ctaaagttac
    12661 gcctgcatgt aattgcgtaa ttccgctgat gatgccaatc acgccgccta ccagcgatgt
    12721 gatgccggaa agcttaatag ccagtctgtc cagaccgagt aaaaacagcg aagccagact
    12781 ggcaaacaga tagaggatca gcgaccaggt taataattgc agggcatcca ttagcgttgc
    12841 tcctgagtga gggaaagggg atcctccccg ccattggcaa accagcttgc ggcaaggcgc
    12901 tgtttttctt tcatctggcg ttgcaggcta tcgtcggtga tcagccgtaa cgcctgattc
    12961 gggcaagcgc gaacgcaggc cggaccttct ggcaagaaat cacacaggtc gcatttcacc
    13021 gcgacggtct ggacacccgg ttcccagcgc agcaacgcat gttggggcgg cacgttttct
    13081 tcgccgtctt tgagtgagcc ctcagcctga aaaacatatt gcgcaggggc attcaccgga
    13141 cggcttcctg aagcgctgat tgcgccaaat gggcagacca cggcgcaaag cttgcagcca
    13201 atacagaggc tttcgttgag ttggatcgca tcatccctct gagagatggc attgaccggg
    13261 cagacctgca ggcaaggggc ttcctcacag tgatgacaca cgacaggggc agtgagtgtt
    13321 gacgtcttcg ccagggccag gcgcgggtgt tgctgtaaac cttgcgtttt atgcacgtcc
    13381 gaacaggcag cgagacaggt attacatcct gtacaccaca gtggttcggc caccacaaag
    13441 cggttcatgt cggctccttg aaaatgatat gaaacattgc atttcatatt gcataaagga
    13501 tgccattctg attaactcaa tatattcaat aagttatgtg ttaactggat tctgaacggt
    13561 acaaaaatga tggtcattaa cgttcagcaa aaaatggatt cgacacccgt gtcgcagggg
    13621 aaagcgagaa tgtgatcttg atcacgagaa gtgaggtatg ctggccgcaa gcgcagccag
    13681 cacggaatgg agcaaagtaa tcaggcgtgt tctttcagcc agttcagcac aacgtcgtgg
    13741 tgattgctgg ttttgaaatc gtcaaagaca tgttcgattt tgccatcagc gtcaatcagg
    13801 aagctgatgc gatgaatgcc atcgtaggtt ttgcccatga aggacttttc accccagacg
    13861 ccgaattgtt cgcacacctg gtggtcctca tcagacagga gcgtaaagtt aagcagctct
    13921 ttttccgcaa aacgggagag tttttcgggt ttatcggtgc tgatacccag cacatcaacg
    13981 cccgcttttt tcaactcatc catgttatcg cgtaagccgc aggcctgtac ggtacagccg
    14041 ggggtcatgg ctttcgggta gaaataaacc agaacacgct gtccctggaa gtcggtcaaa
    14101 ttaacttgtt ctccgtcttg atccggcaag ctaaatttcg gtgcgatatc accggctttc
    14161 agtggattca ttacttaact ccatcctgtt catcatgttg ggaataattg acgacgttaa
    14221 tactgccttg tgcattgagt tctgtacata gggctttgaa cgcttgctca atatttgctg
    14281 cgtccgcaga tgcggggctg tgggcggtta tctgaatatg caactgcgca gcccgttcat
    14341 tttcagcagg ttgcgtgcgc gacaccagct ccgcaatgtt catatgatgc gcgtcgaaaa
    14401 gtgctgtgaa gcgttcaatt aaatgcgggg agtctgccac atcgacctga acccagacag
    14461 atgctggcat tggcggacgc ggacgcgccg tcgtgcgctt catcacgatt aaaagatcca
    14521 gctcggcacc tttcaacggt aacgttgatt caatcagagt aatggcattc catgaaccgg
    14581 aaagcagcat gataaacgtg aactcttctc ccagcatcgc caggcgactg tcttcaatat
    14641 tacagccgca actactgaca tgacgggtga tggtgttcac aattccaggg cgatcggcac
    14701 ccaatgcagt gatcaccaga taatgttgcg atgacagtgt caaactggtt attcctttaa
    14761 ggggtgagtt gttcttaagg aaagcataaa aaaaacatgc atacaacaat cagaacggtt
    14821 ctgtctgctt gcttttaatg ccataccaaa cgtaccattg agacacttgt ttgcacagag
    14881 gatggcccat gttcacggga agtattgtcg cgattgttac tccgatggat gaaaaaggta
    14941 atgtctgtcg ggctagcttg aaaaaactga ttgattatca tgtcgccagc ggtacttcgg
    15001 cgatcgtttc tgttggcacc actggcgagt ccgctacctt aaatcatgac gaacatgctg
    15061 atgtggtgat gatgacgctg gagctggctg atgggcgcat tccggtaatt gccgggaccg
    15121 gcgctaacgc tactgcggaa gccattagcc tgacgcagcg cttcaatgac agtggtatcg
    15181 tcggctgcct gacggtaacc ccttactaca atcgtccgtc gcaagaaggt ttgtatcagc
    15241 atttcaaagc catcgctgag catactgacc tgccgcaaat tctgtataat gtgccgtccc
    15301 gtactggctg cgatctgctc ccggaaacgg tgggccgtct ggcgaaagta aaaaatatta
    15361 tcggaatcaa agaggcaaca gggaacttaa cgcgtgtaaa ccagatcaaa gagctggttt
    15421 cagatgattt tgttctgctg agcggcgatg atgcgagcgc gctggacttc atgcaattag
    15481 gcggtcatgg ggttatttcc gttacggcta acgtcgcagc gcgtgatatg gcccagatgt
    15541 gcaaactggc agcagaaggg cattttgccg aggcacgcgt tattaatcag cgtctgatgc
    15601 cattacacaa caaactattt gtcgaaccca atccaatccc ggtgaaatgg gcatgtaagg
    15661 aactgggtct tgtggcgacc gatacgctgc gcctgccaat gacaccaatc accgacagtg
    15721 gtcgtgagac ggtcagagcg gcgcttaagc atgccggttt gctgtaaagt ttagggagat
    15781 ttgatggctt actctgttca aaagtcgcgc ctggcaaagg ttgcgggtgt ttcgcttgtt
    15841 ttattactcg ctgcctgtag ttctgactca cgctataagc gtcaggtcag tggtgatgaa
    15901 gcctacctgg aagcggcacc gcttgcggag cttcatgccc cggctggaat gattttgccg
    15961 gtgacctccg gtgattatgc aatcccggtg accaacggta gtggtgctgt cggtaaggcg
    16021 ctggacattc gtccaccagc ccagccgctg gcactggttt ctggcgcgcg tacccagttc
    16081 acgggcgata ccgcttcatt gctggtggaa aatggtcgtg gcaatactct gtggccgcag
    16141 gtggttagcg tgctgcaggc gaaaaactac accatcaccc aacgtgatga tgctggtcag
    16201 acactgacca ccgattgggt acaatggaac cgtctggacg aagacgagca gtatcgtggt
    16261 cgttatcaaa tctctgttaa gccgcagggt tatcagcagg cggttacggt taaactgttg
    16321 aacctggaac aggcgggcaa accggttgca gacgcggctt ccatgcagcg ttacagcacg
    16381 gagatgatga acgttatttc cgccggtctg gataaatctg ccactgacgc cgcgaacgct
    16441 gcgcaaaatc gtgcctccac cactatggac gtacaaagtg cagctgatga caccggttta
    16501 ccaatgctgg tcgtacgcgg gccgttcaat gtggtttggc aacgtctgcc agcggcgctg
    16561 gaaaaagtgg gcatgaaagt gaccgacagc acccgttcgc agggcaacat ggccgtaact
    16621 tataagccgc tgtctgacag cgactggcag gaactgggcg cgagcgatcc aggcctggca
    16681 tccggtgact ataaactgca ggttggcgat ttagataacc gcagcagcct acagttcatc
    16741 gatccgaaag gtcatactct gactcagagt cagaacgacg cgctggtagc tgtcttccag
    16801 gctgcgttta gcaagtaaaa atacagggct ggaatcatcc ggcccttttt tctgatatga
    16861 tacgcaaacg tgtgcgtcgg caggaaaacg cgattttagc ggtaattcgc acgaaatttg
    16921 tttgtcggac gtagttcgga taaggcgttc acgccgcatc cgacaaaaca tccggcacac
    16981 cagacagcaa aagattttaa aacgttaatt cacacccagg agtgataaag atgcaaaagc
    17041 aagctgagtt gtatcgtggt aaagcgaaaa ccgtatacag cacggaaaac ccggacctgt
    17101 tggtgctcga attccgcaat gatacgtcag caggggatgg cgcgcgcatt gagcagtttg
    17161 atcgcaaagg tatgttgaac aacaagttca actacttcat tatgagcaaa ctggctgaag
    17221 cgggtatccc gactcaaatg gagcgtctgc tctccgatac cgaatgtctg gtgaaaaagc
    17281 tggatatggt gccggttgag tgtgtcgtgc gtaaccgtgc tgcgggctct ctggtgaaac
    17341 gtcttggaat cgaagaaggt attgagctga acccgccgct gttcgatctg ttcctgaaaa
    17401 acgatgccat gcacgatccg atggtcaacg aatcttactg cgaaaccttt ggctgggtga
    17461 gcaaagagaa cctggcgcgt atgaaagagc tgacctacaa agcgaacgac gtgctgaaaa
    17521 aactgttcga tgatgctggt ctgattctgg tcgacttcaa gctggagttt ggtctgtaca
    17581 aaggtgaagt ggtactgggc gatgagttct ccccggacgg cagccgcctg tgggacaaag
    17641 aaacactgga gaaaatggac aaagaccgtt tccgccagag cctcggtggc ctgatcgaag
    17701 cctatgaagc cgtcgcccgc cgcctgggtg tacagctgga ctgatttttc tgttcatcat
    17761 cttgccgtgc agctggcacg gcaagacaac cgctctcgga atatatgcat tgaacgctca
    17821 ttcctccgcc atttcatccc gctttcctgt ggtaatcttc ctctgaacca cctacgatca
    17881 tttctataat gaatatattg ttgaggtatc tatgcgttgg caagggcgac gtgaaagtga
    17941 caatgtcgaa gacaggcgta acagctccgg cggtccgtca atgggcggtc ccggttttcg
    18001 cctgccaagc ggtaaaggcg ggctgatttt actgatagtc gtgctggttg caggctacta
    18061 tggtgttgat ttaaccgggt tgatgaccgg acagccggtt tcccaacagc aatcaacacg
    18121 gtcaattagc ccaaatgaag acgaagccgc aaaattcacc tcggtgattc tggcaaccac
    18181 ggaagacacc tggggacagc aattcgagaa gatgggtaaa acctatcagc aaccgaagct
    18241 ggtcatgtat cgtggaatga cgcgtaccgg ctgcggggcg ggccagtcca ttatgggacc
    18301 gttctattgc ccggcggatg gcacggttta tatcgatctc tccttctatg atgacatgaa
    18361 agacaaactc ggcgcggatg gcgattttgc ccaggggtac gttatcgccc atgaagtcgg
    18421 tcatcatgtg cagaaattgt taggcatcga gccgaaagtt cgtcaactgc aacaaaacgc
    18481 gacgcaggcg gaagtaaacc gcttatctgt gcgtatggaa ctccaggccg actgttttgc
    18541 cggtgtctgg gggcataaca tgcagcagca aggcgatctg gaagaggcgc tgaacgcggc
    18601 gcaggccatc ggcgatgacc gtttacaaca gcaaagtcag gggcgagtag taccagacag
    18661 tttcactcat ggtacttctc agcaacgcta cagctggttt aaacgtggtt tcgacagcgg
    18721 cgatccggca caatgcaata cttttggtaa aagcatttaa cttccggggc aaggatggct
    18781 gaactgactg cgcttcacac attaacagcg caaatgaaac gtgaagggat ccgccgcttg
    18841 ctggtgttga gcggggaaga gggttggtgt tttgatcatg cgcttaagtt gcgtgatgcc
    18901 ttacctggcg actggctgtg gatttcgccg cagccagatg ctgaaaacca ctgttctccc
    18961 tcggcgctac aaactttact tgggcgcgag ttccggcatg cggtattcga cgcccgccac
    19021 ggctttgatg ccgctgcctt tgcggcactt agcggaacgt tgaaagcggg aagctggctg
    19081 gttttgttac tccctgtatg ggaagagtgg gaaaaccaac ctgatgccga ctcgctgcgc
    19141 tggagtgatt gccctgaccc tattgcgacg ccgcattttg tccagcatct caaacgcgta
    19201 cttacggcgg ataacgacgc tattctctgg cggcaaaacc agccgttctc gttggcgcat
    19261 tttacccccc gtactgactg gcaccccgcg accggcgcac cacaaccaga acaacagcaa
    19321 ctcttacagc agctactgac catgccgccg ggcgtggcag cggtaacggc tgcgcgtggg
    19381 cgcggtaagt ccgcgctggc agggcaactc atttctcgta ttgcgggcag tgcgattgtc
    19441 accgcgcccg caaaagcggc aacggatgta ctggcacaat ttgcgggcga gaagtttcgc
    19501 tttattgcgc cggatgcctt gttagccagc gatgagcaag ccgactggct ggtggtcgat
    19561 gaagccgcag ccatacctgc gccgttgttg catcaactgg tatcgcgttt tcctcgaacg
    19621 ttgttaacca ctacggtgca gggctacgaa ggtaccggac gtggtttttt gctgaaattt
    19681 tgcgctcgct ttccgcattt acaccgtttt gaactgcaac agccgatccg ctgggcacag
    19741 ggatgcccgc tggaaaaaat ggttagtgag gcactggttt ttaacgatga aaacttcacc
    19801 catacaccac aaggcaatat cgtcatttcc gcatttgaac aaacgttatg gcgaagcgat
    19861 ccagaaacgc cgttaaaggt ttatcagtta ttgtctggtg cgcactaccg gacctcgccg
    19921 ctggatttac gccggatgat ggatgcacca gggcaacatt ttttacaggc ggctggcgaa
    19981 aacgagattg ccggggcgtt gtggctggtg gatgagggcg gattatctca agaactcagt
    20041 caggcggtat gggcaggttt tcgtcgcccg cggggtaatc tggtggccca gtcgctggcg
    20101 gcgcacggca gcaatccact ggcggcgaca ttgcgcggac ggcggggcag ccggattgca
    20161 gtccatccgg cgcgtcagcg cgaaggcgtt gggcaacagc tcattgccag cgctttgcaa
    20221 tataggcctg gcctcgacta tctttcggtg agttttggtt acaccgggga gttatggcgt
    20281 ttctggcaac gctgcggttt tgtgctggtg cgaatgggta atcatcgtga agccagcagc
    20341 ggttgctata cggcgatggc actgttaccg atgagtgatg cgggtaaaca gctggctgaa
    20401 cgtgagcatt accgtttacg tcgcgatgcg caagctctcg cgcagtggaa tggcgaaacg
    20461 cttcctgttg atccactaaa caatgccgtc ctttctgacg acgactggct tgaactggcc
    20521 ggttttgctt tcgctcatcg tccgctatta acgtcgttag gttgcttatt gcgtctgcta
    20581 caaaccagtg aactggcatt accggcgctg cgtgggcgtt tacagaaaaa caccagtgac
    20641 gcgcagttat gtaccacact taaactttca ggccgcaaga tgttactggt tcgccagcgg
    20701 gaagaggccg cgcaggcgct gttcgcactt aatgaggttc gcactgagcg tctgcgcgat
    20761 cgcataacgc aatggcaatt ttttcactga ctccttcaat tttctcccat ggtcattgtg
    20821 agtcagcggc gtaaaattaa tgacatcagt taaggagttc gccatgaaac atgaccattt
    20881 tgttgttcaa agcccggata aacctgcgca acagttgtta ctgcttttcc acggtgtcgg
    20941 ggataacccg gtggcaatgg gggaaatagg cagctggttt gcgccgctat ttcctgatgc
    21001 gctggtggtg agtgtcggag gcgcggagcc gagcggtaat ccggcggggc gtcagtggtt
    21061 ttcggtgcag ggtattacgg aagataatcg ccaggcgcgt gtggatgcca tcatgccgac
    21121 gtttattgag atggtgcgct actggcagaa acagagcgga gtaggggcta acgccacagc
    21181 gctaatcggt ttctctcaag gcgcgattat ggcgctggag agcattaaag ccgaaccggg
    21241 ccttgcttca cgcgtcattg cttttaacgg acgctatgcg agcctgccgg aaactgcgtc
    21301 tacagccacg acaattcacc tgattcatgg cggtgaagat ccggttatcg atctggcgca
    21361 tgcggttgcg gcgcaggaag cgttaatcag tgccggtggt gacgtgacgc tggatatcgt
    21421 ggaggattta ggccatgcga tcgacaatcg cagtatgcaa tttgccctgg atcacttgcg
    21481 ttataccatt ccgaaacatt atttcgacga agcgctaagt ggcggtaagc ctggcgatga
    21541 tgatgtgatt gaaatgatgt aaaagacaga ttacctggcc tgataagacg cgtgagcgtc
    21601 gcatcaggca tcgttcacca ttgccggatg cggcgttaac gccttatccg gcctacggtt
    21661 gagcactcag cttacttctt cggttgatcc tttttcggcc agtcgtcgtc atcgtcccac
    21721 ttatcgttga aatcacgatg cgggggaagc tccggcttgt tggcgagaaa tttcttgtgg
    21781 tcgacgcgct tgagatcttt aatcacgttc agcaggacgc ctaccaaaaa gacaatcacc
    21841 agaatccacc aatatttagc cagccagtcc atgcttattt cctcttgcag aaccactcat
    21901 caggcgacga gctgttccat gatacgttga tacatacggg caagtagctg caggtcggca
    21961 gcgttcacac attcattaat tttatgaata gtggcattga ccggcccgag ttccaccacc
    22021 tgcgccccca tgcgagcaat aaagcgcccg tcggacgttc cgcctgtggt cagtagctgc
    22081 ggtttaattt cattatagtg ctcaaccgcg ttaacgaccg catccaccag tttaccgcgc
    22141 gcggtcaaaa atggctgccc ggaaagccac caatccaccg tatagcgcag ttgatgtttt
    22201 tcaagcaggg caagcacctg cgctttgatc atctcatcag tcagttcggt gctgaagcgg
    22261 aagttaaact gcacaaacag ttcacccgga ataacgttgt tactgcccgt tcccgcctga
    22321 atattggcaa tctgcatact ggtcgccggg aagaattcat tgccctgatc ccactcaata
    22381 gccactaatt cattaaggaa aggtgctgcg cgatgtactg gattgtcagc cagatgtggg
    22441 taggccacat gcccctgcac gccatgaatg gtaaggttgc aggttaacga tccgcgacga
    22501 ccatttttca ccacatcacc aaccacttcg atactcgacg gttcgccgac cagacagtaa
    22561 tcgagacgct cattacgcgc cattaacgct tcgacgactt ttaccgtacc gttgtgggca
    22621 ctggcttctt catcagaggt gatcagaaat gccagtcgtc ccgtatggtt gggatgttgt
    22681 gcgacaaaac gttctgctgc taccaccatc gccgccagcg agcctttcat atctgccgca
    22741 ccgcgcccga ataacatgcc atcacgaatg gtgggttcaa acggcggatt gatccaacga
    22801 tcggcgtcgc caggcggcac cacgtcggta tgcccggcaa aggccaatgt ttcaccttgc
    22861 ccacgccatg cccaaaaatt ctgcgtatcg gcaaagtcca tgcgttcaac ggtaaaaccg
    22921 atcgcctgca aacgatcaat caacaaagcc tggcatcctg cgtcatcagg actcagggaa
    22981 gggcggcgaa taagctgttg tgtcagctca ataaccgggc acgacataga ctacacctca
    23041 tggaaaaatt gctgataact ggaatcactg aaacccagca gcataggctt accgggcgcg
    23101 cagagcaatg gacgtttgat aattgcaggc atttcagtca ttaatgccgc cgctgaggcc
    23161 gcatcggtga ttttattgcg ggtggtttcg tccagtttac gccaggttgt accacgggtg
    23221 ttgagtaacg cctcccaacc taattcgttg ataaaaccgt tcaataattc gctgtccagc
    23281 ccatcgacgc ggtaatcatg aaaacgatag tcgatgttat tggcttccag ccaacggcga
    23341 gcctttttaa cggtgtcaca atttttgata ccgtaaagtg taaccataat gaatcctttt
    23401 tcgatttatg agaataaata ctcatttaag ggtgaaaaca
//
LOCUS       DAZPDU010000054        23237 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZPDU010000054 DAZPDU010000000
VERSION     DAZPDU010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23237
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.054"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(40..240)
                     /gene="glgS"
                     /locus_tag="ACHURS_001925"
     CDS             complement(40..240)
                     /gene="glgS"
                     /locus_tag="ACHURS_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFR1237669.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            509..1138
                     /locus_tag="ACHURS_001926"
     CDS             509..1138
                     /locus_tag="ACHURS_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFR1237670.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYNSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGVKVLIICRLSATRYLAENNPWPQIL"
     gene            1165..2826
                     /locus_tag="ACHURS_001927"
     CDS             1165..2826
                     /locus_tag="ACHURS_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFR1237671.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            3065..3124
                     /locus_tag="ACHURS_001928"
     CDS             3065..3124
                     /locus_tag="ACHURS_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR1237672.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            3441..3500
                     /gene="ibsE"
                     /locus_tag="ACHURS_001929"
     CDS             3441..3500
                     /gene="ibsE"
                     /locus_tag="ACHURS_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HFR1237673.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(3620..5053)
                     /gene="hldE"
                     /locus_tag="ACHURS_001930"
     CDS             complement(3620..5053)
                     /gene="hldE"
                     /locus_tag="ACHURS_001930"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFR1237674.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPSSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(5101..7941)
                     /gene="glnE"
                     /locus_tag="ACHURS_001931"
     CDS             complement(5101..7941)
                     /gene="glnE"
                     /locus_tag="ACHURS_001931"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFR1237675.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEEPLSAQAKSVLTFSDFVQD
                     SIIAHREWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSCSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMNNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLISDFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALNHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSTEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGRTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSGDCFTAERDLVRASWQKWLVEE"
     gene            complement(7964..9265)
                     /gene="ygiF"
                     /locus_tag="ACHURS_001932"
     CDS             complement(7964..9265)
                     /gene="ygiF"
                     /locus_tag="ACHURS_001932"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFR1237676.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQFPSEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSHHAAELKSVFCQPLGDLYRDQLPRLTRDIDSILLLAGYYDPVVAH
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            9507..10127
                     /locus_tag="ACHURS_001933"
     CDS             9507..10127
                     /locus_tag="ACHURS_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFR1237677.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            10191..11429
                     /gene="cca"
                     /locus_tag="ACHURS_001934"
     CDS             10191..11429
                     /gene="cca"
                     /locus_tag="ACHURS_001934"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFR1237678.1"
                     /translation="MKIYLVGGAIRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTDFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(11592..12413)
                     /gene="bacA"
                     /locus_tag="ACHURS_001935"
     CDS             complement(11592..12413)
                     /gene="bacA"
                     /locus_tag="ACHURS_001935"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFR1237679.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDISMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(12504..12872)
                     /gene="folB"
                     /locus_tag="ACHURS_001936"
     CDS             complement(12504..12872)
                     /gene="folB"
                     /locus_tag="ACHURS_001936"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFR1237680.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAM
                     ARAANVGVIIERGNNLKENN"
     gene            12977..13594
                     /gene="plsY"
                     /locus_tag="ACHURS_001937"
     CDS             12977..13594
                     /gene="plsY"
                     /locus_tag="ACHURS_001937"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFR1237681.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(13607..14539)
                     /gene="ttdR"
                     /locus_tag="ACHURS_001938"
                     /gene_synonym="dan"
     CDS             complement(13607..14539)
                     /gene="ttdR"
                     /locus_tag="ACHURS_001938"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFR1237682.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            14746..15657
                     /gene="ttdA"
                     /locus_tag="ACHURS_001939"
     CDS             14746..15657
                     /gene="ttdA"
                     /locus_tag="ACHURS_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFR1237683.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            15654..16259
                     /gene="ttdB"
                     /locus_tag="ACHURS_001940"
     CDS             15654..16259
                     /gene="ttdB"
                     /locus_tag="ACHURS_001940"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFR1237684.1"
                     /translation="MKKILTTPIKAEDLQDIRVDDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            16308..17771
                     /gene="ttdT"
                     /locus_tag="ACHURS_001941"
     CDS             16308..17771
                     /gene="ttdT"
                     /locus_tag="ACHURS_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFR1237685.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLIVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(17814..18827)
                     /gene="tsaD"
                     /locus_tag="ACHURS_001942"
     CDS             complement(17814..18827)
                     /gene="tsaD"
                     /locus_tag="ACHURS_001942"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFR1237686.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            19064..19279
                     /gene="rpsU"
                     /locus_tag="ACHURS_001943"
     CDS             19064..19279
                     /gene="rpsU"
                     /locus_tag="ACHURS_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFR1237687.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            19390..21135
                     /gene="dnaG"
                     /locus_tag="ACHURS_001944"
     CDS             19390..21135
                     /gene="dnaG"
                     /locus_tag="ACHURS_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFR1237688.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            21330..23171
                     /gene="rpoD"
                     /locus_tag="ACHURS_001945"
     CDS             21330..23171
                     /gene="rpoD"
                     /locus_tag="ACHURS_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFR1237689.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         5654 a         6331 c         5907 g         5345 t
ORIGIN      
        1 ctaatacaaa cactaaaacg ggccatcagg cccgttatat cagtgctcta actccagctc
       61 tcttgcctgc atcatctgtt ggcaatacca ggcataacgt gcgcaaaacc aggttctatg
      121 ttcttcatcc atgttaccag taacggcaag aatatcgacc gggcgacctt ctgcgtgcat
      181 tctggcaaaa ctacgcgcca ggaaatcgaa attatttaaa gaattaagac tatgatccat
      241 aagcacatcc tccggttatc gcataccttt taaggccata tgctttttct gatttcagta
      301 ttccccaatc agtgtagtgc ctttaaacat aacgtgcgta aatatattgt ccccgatcac
      361 actttttagt gaaaaataaa aaactatatt aaaaaacttt ctcttccaga acttctctaa
      421 catgtgttat tattaacaaa gataaatgta agcaaggaaa ctatcccaaa gtaaatattg
      481 cgtcactaaa tggacattgg agtgacatat gattttattc gccgactata atacccctta
      541 tcttttcgct atttcctttg tactgttgat cggcctttta gaaattttag cgcttatctg
      601 cggtcacatg ctctccggtg ctcttgatgc acatcttgac cattataatt ccataaccac
      661 aggtcatatt agccaggcac ttcattatct caatatcgga agattgccag ctctcgtcgt
      721 tctctgcttg ctggctggtt tttttggtct tattggcatc ctgttgcagc atgcctgcat
      781 catggtctgg caatcaccac tgtcgaacct gttcgtggtt cctgtcagtt tactgtttac
      841 gattattgcg gtgcattata ccggtaaaat agttgcgccc tggattccac gcgaccatag
      901 ttcagctatt acagaagaag aatatattgg tagcatggca ttaattaccg gtcatcaagc
      961 cacttcaggt aatccctgcg aaggaaaact caccgatcaa tttggccaaa tacattattt
     1021 actactggag cctgaagaag gaaaaatttt cactaaagga gtcaaagtgt taattatttg
     1081 ccgactttct gcaacgcgat atttggcgga aaacaatccc tggccacaaa tattgtaatt
     1141 tattaatcaa aaggaaattt aataatggat gatattgtta attctgtgcc ctcctggatg
     1201 tttaccgcga ttattgccgt atgcattctg tttattattg gaattatttt cgccaggctc
     1261 tatcgtcgcg cttcggcaga gcaagctttt gttcgtactg gtttaggtgg gcaaaaagtg
     1321 gtgatgagcg gtggcgcaat cgtgatgccg atctttcatg aaataatccc catcaatatg
     1381 aatactctga agctggaagt cagccgctca accattgata gcctgattac gaaagatcgt
     1441 atgcgtgtcg atgtagtagt cgctttcttt gtacgggtaa aaccgtctgt agaaggcatc
     1501 gccactgcgg cgcagacgtt ggggcaacgc acgctatcac cagaagactt acgtatgttg
     1561 gttgaagata aatttgtcga tgccctccgt gcaacagctg cacaaatgac catgcatgag
     1621 ttacaggata cccgcgagaa cttcgtgcag ggagtacaaa atactgtcgc agaagaccta
     1681 tcgaaaaacg gtcttgagct ggaaagcgtt tcacttacca actttaacca gacctcgaaa
     1741 gaacatttca atccgaacaa tgcctttgac gccgaaggtt taaccaaact gactcaggag
     1801 acggagcgcc gtcgtcgcga acgtaacgaa gttgaacagg atgtagaagt tgcggtgcgt
     1861 gagaaaaacc gtgatgcact ttcgcgcaag ttggagattg aacaacaaga agcgtttatg
     1921 acgcttgagc aggagcagca ggttaaaacc cgtactgccg aacagaatgc acgtattgcg
     1981 gcttttgaag ctgaacgtcg tcgtgaagca gagcagacgc gaattctggc tgaacgacaa
     2041 attcaggaaa cggagatcga gcgcgagcag gccgtccgct caagaaaggt tgaggctgaa
     2101 cgtgaagttc gtattaaaga gatcgaacag cagcaggtca ccgaaatcgc caaccagacg
     2161 aaatcgatcg ctattgccgc caaatcggaa caacagtccc aggcagaagc gcgtgctaac
     2221 cttgcactcg cagaagcagt aagtgcgcaa caaaacgtag aaaccactcg ccagaccgca
     2281 gaagccgatc gtgctaaaca agttgcccta atcgctgccg cgcaggatgc agaaaccaaa
     2341 gcggttgaac tgaccgtgcg ggcgaaagca gaaaaagaag ccgcagagat gcaggcggcg
     2401 gctatcgttg agttagccga agctacacgt aaaaagggtc tggcggaagc agaagcacaa
     2461 cgtgcgctga acgatgctat caacgtactt tctgatgaac aaaccagcct taaattcaaa
     2521 ctggccttgt tgcaggcgct acctgcggta atagagaaat ccgttgagcc gatgaagtcc
     2581 attgacggta tcaagattat tcaggtcgat ggtctgaatc gtggcagcgc tgcgggtgat
     2641 gcaaacacgg gtaatgtggg gggcggaaac ctagcggaac aagcattatc agccgctctc
     2701 tcttaccgca cacaggcacc gctgattgac tccttgctca atgaaattgg cgtttcaggc
     2761 ggctcactgg cggcattgac ttcaccctta acctcaacaa ctcccgtcgc cgaaaacgta
     2821 gaataaattt tcagctatga acgtccctgg cacaaaaagc cggggatttt ttatatctgc
     2881 gttccgctaa aaactgcaaa tgatcaggcc gttgcaacgt tttgcgtgac cgctcgggga
     2941 aggcaaaact gcctctggga aagcgttgcg cgaggtccgg cgctcatcaa caatcgggga
     3001 gtcagcaaag ggctgaaacg ggaaagcccc tcccgaggaa ggggccttac agaaggaaag
     3061 ggttatgatg aagctcgtca tcatactgat tgtgttgtta ctcgtaagtt tcgcagctta
     3121 ttaacagcca atcagagggg ggagaaatcc tccctcaccc ttgttccttt actttaggtt
     3181 gaaaaaaaat cagcgtcaag aaggtttcat taacacatat ccctggccca aaaagccggg
     3241 gattttttat atctgcgttc cgctaaaagg tgcaaatgct caggacgttg cagcgttttg
     3301 cgtgatcgct cggggaaggc aaaactgctt cagggaaagc gttgcgcggg gtccggcgct
     3361 catcaacaat cggggagtca gcaaggggct gaaacgggaa agcccctccc gaggaagggg
     3421 ccttatagaa ggaaagggtt atgatgaagt tcgtcatcat actggttgtg ttgttactgt
     3481 taagtttccc gacttactaa caactgatca gagggggaag aaatcctccc ttacccttgt
     3541 tcctttactt taggttgaaa aaacaacagc gtcaataggc ctgcctgcta caaagcgaga
     3601 tctgtgaacc gctttccggt tagccttttt tatcctgttg gatcttcttg atgatgttag
     3661 tggtcgagca accgtcttcg aagttgagca ccagcacttc gccgccgttg gcccagactt
     3721 ctttactccc ggcgatctct tctggtttat agtcgccgcc cttcaccagt aaatctggca
     3781 ggatcccggc gatcaagcgc tgcggcgtgt cttcttcaaa cgacaccacc cagtcgaccg
     3841 cttccagtgc gcccagcaca atcatacgct gttcgagcgg gttaacgggg cgggaatccc
     3901 ctttcagccg tttggtggag gcatcgctgt tcacagcgac aatcagacgg tcacccagtt
     3961 tgcgagcatt tgccagatag gagacgtgcc cggcgtgcag gatgtcaaag acaccgttgg
     4021 tcattaccac cttttcgccg cgtttacgcg ctgccgctac ggccagcttc agttcctctt
     4081 cggtcatcac gccaaagccg gtatctgcgc gcccacgcac tgcattttcc agctcgatcg
     4141 gcgaaaccgt ggaggttccc agtttgccga ccaccacgcc agccgccgca ttggcaaaga
     4201 agcaggcttc ttccagcgaa ttacccgctg ccagcgttgc cgccaggacg ccaatcaccg
     4261 tgtcgcccgc accggtaacg tcatacactt cctgcgcctg ggttggcata tgcagcggcg
     4321 ctttacccgg ttgcagcagc gacataccct gttcggaacg ggtcactaac agagccgaga
     4381 gttcgtaatc ggcaatcagt ttcatgccgc gctcaacaat ctcttcttcg gtcttacatt
     4441 taccgacaac agcttcaaat tccgagagat tcggcgttaa cagcgtagcg ccgcggtagc
     4501 gctcaaaatc ggtacctttt ggatcaatca gcaccggaac gcccgcttta cgcgccagtt
     4561 ggatcatctg ctgtacgctt gccagcgcac ctttggcgta gtcagaaagc accagcgcgc
     4621 caatcgaact cagcgcctga ttaatccgct cgtgcagcgg ctgcggatca acgccttcga
     4681 aaccttcttc gaaatccaga cggatcagct gttggttgcg ggaaagtacc cgtaatttgg
     4741 tgatggtcgg atgcgtcggt acagaaacga agtcgcattt gacgttgacg tcggccagag
     4801 atttactcag cgcgcgcgct gcatcgtcaa tgcccgtcaa cccgaccagg cgtgcattag
     4861 caccgagaga agcgatattc atcgccacgt tagccgcgcc gcccggacgt tcttcgatgg
     4921 tattcacttt aaccacgggc accggcgctt ccggcgagat acggctggag ggaccgtacc
     4981 agtaacgatc cagcattaca tcaccaacca ccatcactcc tgcacgttca aactctggca
     5041 gcgttacttt cattcctgtc tcctgagaga ttcaaaattt gcgcgcgata ataccatact
     5101 tcattcttcc accagccact tctgccagct tgcccgcacc agatcacgct ctgcggtgaa
     5161 gcaatccccc gacacatggc ccggcaattc ctgtaatgcc agatgatgaa gttcatcgcg
     5221 cagcgtcgtg taagcacggg tcagcgccat cgcttcctgc tcttccataa tgtcgttttg
     5281 cgccagcagc tccagaatac gcacgttgtc tgaccagcgc gttaacttcg gtttttcatg
     5341 agcgtagcgc aacaccagat attgggtaat aaattcgata tcggtaattc cgccttcatc
     5401 agctttgata tcaaagcgat cgcgatgttt attgccgaga tgagcgcgca ttttctcgcg
     5461 catttcccgc acttccgttt gcagagttct accttcacgc ggcagcgtca taatctcgcg
     5521 acgcactgcg tcaaagtgcg cggtgagctg cggatcgccg tacactaccc gcgcacgcac
     5581 cagcgcctga tgttcccacg tccaggcctc gtttttctga taatcggcaa atgcttctgt
     5641 ggaggtaacc agcattcccg ccgccccgga cgggcgcagc cgcgcatcca cttcgtacaa
     5701 aatgccggaa gaggttcgcg tgctgaacag gtgcataatg cgttgcgcca gacgaagata
     5761 aaactgtcgt ccgtcgattt cccgctcgcc gtcagtcatc gcatccattg ggcaatcatg
     5821 gaggaagatc aggtcaagat cggaactgta gcctaactcc cagccgccca gcttgccgta
     5881 gccgaccact gcaaaaccac gcccttcgcg atcgttgagg tgattcggct taccgtagcg
     5941 ggcaaccatt tgaacccacg cctgctgaac gacggcatct atcatggctt ccgccagcca
     6001 ggttaagtga tcgctcactt tcatcaccgg tagcgtacca gcgatatccg ctgcggcgat
     6061 gcgtaacagc tgcgcctgtt tgaactgacg cagcgcctca agctgttgct cttcgtcatc
     6121 ttccggcacg cgcagcaaat actggcgcaa ctcatcgcgg taggcatcgg tcgccgtcgg
     6181 ctggtaaaga gtgtttggat cgagcaattc atccagcaat aatggataac gcgccagctg
     6241 gctggcaatc atcggcgatg cggcacacag agaaatcaaa tggttaagcg ccgcagggaa
     6301 ttcactgagc aattctaaat aggtggtgcg ggtaacaatc cccaccagca aggcggtaat
     6361 gcgcgacagc gtgacagcag cgtcttcacg cgcacagaca tcacttagca gatgcggcat
     6421 cagatggtcg agcacctgac gtcctcgcgg cccgatggtg cgcttatcca gctctttgcg
     6481 gaaatcggaa atcagcgtta gcacctgttt gcgatcatcc tcgctaagat gcgccagcac
     6541 tggcgtagtg tcatcttcct gcaacgcatc ctgccacagc tcacgccact gttccgacag
     6601 cgactcttcc tgagtttcac tttcgtcgtc gccaatcaat tcattaaaca cccggcgcac
     6661 attgttcata tgtgcggtca gcgccccggt cagttgcggc cagtcagcaa agtccatcgc
     6721 ccacgccagc cgcgcacggt taagctcatc agaaggaagc gtctgggttt gttcgtcgtt
     6781 aatgctttgc agcaggtttt ccagacgccg caggaacaga tacgccactc gcaattgttc
     6841 agcatcgttt tcagaaagca gatgcagcgc agcaatggcg ctgagcgttg gcagtaaaga
     6901 gcacgattgc agcgacggtt cgcgcccgcc gcgaatgagc tggaatacct ggacgataaa
     6961 ttcaatttcg cgaataccac ccgcgccaag tttgatgttg tcggtcaaac cgcgccgacg
     7021 cacttcacgg gcaatcattc ctttcatgtt gcgcagcgac tgaatcacgc tgaaatcgat
     7081 gtaacgacgg aaaacaaacg ggcgtagcat cgcacgcaac tcgttagcat agacgccttc
     7141 gctatcgccc ataatccgcg ctttgaccat cgcgtaacgc tcccagtcgc gcccctgctc
     7201 ctggtaataa tcctccaacg cggcaaagct cagcaccagc gggccacttt cgccaaacgg
     7261 acgcagtcgc atatccacgc gatagacgaa gccatcctgc gttggttgat ccagcacttt
     7321 aatcagccgc tgccccatgc gggtaaaaaa ctgcgcgtta tccagttccc ggcgtccgcc
     7381 ctgcgtacaa ccatgttccg gccaggcaaa aatcagatcg atatcagagg agaaattcag
     7441 ttccccgccg cccagcttac ccatgcctaa gatcagcagc ggttgcgctt cgccctgcgc
     7501 attgcacggc gttccccact cgcggcagca ggcgtcatac agccagtcac gcgccgcaac
     7561 aatcagcgtc tccgccagat aactgagctg ctgcaatatg ctctcttcag taaccagtgc
     7621 cagcgtttgc gcccatgcga tgcgcaccat aatgcgccgc cggaatagcc gcagctcgcg
     7681 cattaacccg gcttcgtcac tcacattact gagcgcctcc tgcaaccatg cagcgtaatg
     7741 ctgccattca tcggcctgcg gcggttggct ttccagttcc gtcaaccact ctcgatgcgc
     7801 aattatgctg tcctgcacaa aatcactgaa agtaagtact gacttcgcct gtgcacttag
     7861 tggttcctcg gctaaaggct ctggcagccg ctcaacaacg gtctgccagt actgctgtaa
     7921 cggtgaagag agcggcttca taagcgattt tatccttgcc tgattaacgt tttccgctgt
     7981 gcaaccagaa cggttcctga ttgtttgcct cattacggaa atgctcaatt tcaatgcgtt
     8041 gcccggtcgc aatagcgtga cgcagcccct gccagttctc cagccaggca tgcgcgacga
     8101 caggatcata gtaacccgcc agcaacagta ttgagtcaat gtcacgcgtc aggcgtggca
     8161 actggtcacg gtagagatcg cctaacggct ggcagaaaac gcttttcagt tcagcggcat
     8221 ggtgggaaag atggatatcg gcaaagcgtt tgaaggagtc gctgattttg ccctgcgctt
     8281 tggcatctaa aaatggctgc catgctttgc ttaccaacca ttcggtcaac gccagcttcg
     8341 ccattgccgt ttcggtagag tagaccgccg tcacggcaga aaccgcagaa gcaatggtcg
     8401 cttcgcattg ggtcagcaaa tcacgtaagt gagtgctcgc tttacgcggc acaataccac
     8461 cgaacagcat cagcgtatga cggaccaggc taatggctgc cagcacctgt tctttcgccg
     8521 catcgttacc gcgtacccac agttcttcat gatactgcca ttgcgctaac gccagctcga
     8581 gcgccgcttc cagcccctgt tctacatcgg cttttgccgc aacatgcaaa atggtggtcg
     8641 gtttgatttc acgcgccgga ttgccctgcg ccagatgata gccacgcgcc gctttgctca
     8701 ggctgccctg gcgtaatccg gtttgcgata ccagttggtt cgccagtttc agcaccgcgc
     8761 gcgtgtcgcc gctaagcagt tccagttcca gctcacagat aggttcagca aattcccccg
     8821 ctttcacctc cccctgatcg agagcgattt caattcggct gccatcgacc gccaccagcc
     8881 atttttcgcg ataaaaatcg gtgctgaaca gcggttgtac gcgggaagcg agatcggcag
     8941 gcaattcacc gttcggccag acttccgagg gaaactgcgc caggtcgagc gtcggttcgc
     9001 tcaacgccac gttatattcc gggcgctggt gtaagccgcc tgtcactctt cctgcaactt
     9061 tcatggtcat ctcatagcga ccgttttcgc cacgaatacg taagcccata tcgtgcctac
     9121 gcagccagtt atccggcgtt tcgtagtaaa tattcagcaa ctgcacgggg tcatggtgct
     9181 cgccgcccag cgtattgaga tggtcacgca acgcctcaac ggcactgtga ttaacaataa
     9241 actttaattc gatttcctga gccatggcct ttacttatcg gttatgtcac ataagtgacg
     9301 atgaacggcg aacttaatgc gatctttttt gtcagtagat agtattttgc gccaaattgc
     9361 catgcaacgg gcaatttgac gggcgtaaaa gtttgaagca gtggcaagac ctgacacaga
     9421 ggattccgat tgatgtcgaa tgctttgcgt agcgacactg atgccactac tatcgttcca
     9481 ctttccatga caataacgac agcctgatgc caaaattacg cctgatcgga ttaactttac
     9541 tcgcacttag cgcgactgcc gtctcacacg ctgaagaaac gcgctatgtt tctgacgaac
     9601 tgaatacctg ggtccgtagc ggtccgggag atcattatcg cctcgtgggc acggttaacg
     9661 ccggcgagga agtgacctta ttacaaactg acgccaacac caattatgcc caggtgaaag
     9721 acagctctgg ccgtaccgcc tggatcccgt tgaaacaact tagcactgag ccaagcctgc
     9781 gctcccgtgt gccagatctg gaaaatcagg tcaaaaccct gaccgataaa ctcaccaata
     9841 tcgataacac ctggaatcag cgcaccgcag aaatgcagca aaaagtggcg cagagcgaca
     9901 gcgtgatcaa cgggttaaaa gaagaaaatc agaaactgaa aaacgagctg attgtcgcgc
     9961 agaaaaaggt cgatgccgcc agcgtacagc tggatgacaa acagcgcacc atcatcatgc
    10021 aatggtttat gtatggcggt ggcgtgctgg ggcttggctt gctgctcggt ctggtactgc
    10081 cgcacctgat cccaagccgc aaacgcaaag atcgctggat gaactaaatc gccttcttcg
    10141 ttgcactgtc ttattattag gaattgtgta tttgacgaag ggatgatggc gtgaagattt
    10201 atctggtcgg tggtgctatt cgggatgcat tgttagggct accggtcaaa gacagagatt
    10261 gggtggtagt cggcagtacg ccacaggaga tgctcgacgc gggctaccag caggtaggcc
    10321 gcgattttcc tgtgtttcta catccgcaaa cgcatgaaga gtatgcgctg gcacgtaccg
    10381 aacggaaatc cggttccggt tacaccgatt ttacttgcta tgccgcaccg gatgtcacgc
    10441 tggaagatga tcttaagcgt cgcgatctga ccattaatgc gctggcccag gacgataacg
    10501 gcgagattat cgacccgtac aacggtctgg gcgatctgca aaatcgtctg ttgcgccatg
    10561 tttcccccgc ttttggcgaa gatccgttac gcgtactgcg cgtggcgcgt tttgctgcgc
    10621 gttatgccca cctcggtttt cgtattgccg atgaaactct gacgctgatg cgcgagatga
    10681 cccatgcggg tgaactggaa cacctgacgc ctgaacgggt atggaaagag acggaaagcg
    10741 ccctcaccac ccgcaatcca caggtgttct tccaggtact gcgcgattgc ggcgcactgc
    10801 gcgttttatt cccggaaatt gacgcactgt ttggcgttcc ggcccctgcc aggtggcatc
    10861 cggaaatcga cacgggtatt cataccttaa tgacgctctc aatggcggcg atgctgagtc
    10921 cgcaggtcga tgtccgtttc gcaactttat gccacgatct cggtaaagga ctgacgccgc
    10981 cagaactttg gccgcgtcat catggtcacg gtccggcggg tgtgaaatta gtggaacaac
    11041 tctgccagcg tctgcgcgtg ccaaatgaaa ttcgcgattt agccagactg gtggctgagt
    11101 ttcacgatct catccacacc ttcccaatgc tgaacccaaa aactatcgtc aaattgtttg
    11161 attccatcga cgcctggcgt aaaccgcagc gtgtcgagca actggcgctg accagcgagg
    11221 ctgacgtacg cggcagaacc ggttttgaat cagcggacta cccgcaaggc cgttggttgc
    11281 gcgaagcctg ggaagtggcg cagtcagtgc cgacaaaagc ggtcgttgaa gcgggattta
    11341 aaggtgtgga gattcgcgag gagctgaccc gacggcggat tgcggcggta gccagttgga
    11401 aggaacaacg ttgcccaaag cctgactgag tataaaacta aacgttaaca cgaaccatcc
    11461 cctcgcccct tcggggagag ggttagggtg aggggaacag accagcaccg gtgcgaacaa
    11521 tcacccccac cccggccctc tccctacaga gcgagggaga aaaccaggcc tgctcccgaa
    11581 ccttttctca atcaaaagaa cacgacgtac accgcagccg ccacaataaa gcgataaatg
    11641 gcgaacggga taaacgaaat gcgcttaatc aattgcagga aggttttaat cgctatcagc
    11701 gccaccacaa aagcggtgat aaacccaacg gcaaacatcg agatatcgcc ggttgtcagg
    11761 aagccccagc ttttgtagag atcgagcgcc gttgcgccca tcatcatcgg taccgccagc
    11821 aggaacgaga actcggaagc agcgtaacgg ctcacgccca tcagcatccc acctgaaatg
    11881 gtggccccgg aacgggagaa acccggccac agcgccagac actggaaaca gccaatcata
    11941 aacgcctgac gataggtcat atcatcaaga cccggcgcac gcggctcttt cggcttcagg
    12001 cattcggcgg caatcagcaa caaaccgcca acgaccagtg catacatcac atttatcggg
    12061 ttaaacaatg acttaatcgt gtcgtggaac aacagcccca gtaccaccgc cggaatcatc
    12121 cccagcaaaa tgtggatcag cgttaaacga cctttgcttt ctccttcgtg ctgcaacggg
    12181 cggccaaagt ggatgccaat caggccaaac agacgccgcc agaacatcac tactaccgcc
    12241 agaattgatc ctaactggat cacaacttca aaggttttcg ccgtgtcgcc ctcgaacccc
    12301 aacaagtgac cgacaataat catatggccc gtgctggata ccggcagaaa ttctgtcaat
    12361 ccttcgacca cacccaatat tgccgctatc agcagcgagt gcatatcgct catcaataaa
    12421 cccctaaatt attaaaatgt accgcttgtc cggactactg cagtaagacc aggttataac
    12481 cgtttggttt aacagctgta aaattaatta ttttctttca gattattgcc acgctcaatg
    12541 attacaccaa cattcgccgc ccgcgccatt gcgcctggct tgctgagttt gatgcgcacc
    12601 cacggcgagt tgaagcgtgc taacagcagc tccgccacct cttcagccac gcgttccacc
    12661 agcgcaaaac gcgccccctc gacgtggctg accaccgttt ctgcaatgtc agcgtaactg
    12721 aggcaatccg ccacatcatc acttttcgcc gctttacggt tatcccatgc catttcgata
    12781 tcgaacacta acttctgttc gatggtctgt tcccagtcgt aaacaccaat agtggtgatt
    12841 accgaaagtt gctctataaa tacaatatcc atcacgtcct gcctgctttt tggctaaccg
    12901 gataccactt ccggcgaaat gtgcgtatta tccacagatt catcgttgaa cacgaatttt
    12961 caaaacggaa cagcttatga gtgcaatcgc gcctggaatg atcctcatcg cgtacctctg
    13021 cggctccatt tccagtgcca ttctggtttg ccgcttgtgt gggctgcccg atccgcgaac
    13081 cagcggctcc ggcaatccag gcgcaaccaa tgtgttacgt atcggtggca agggagcagc
    13141 cgtagcagta ctgattttcg acgttctgaa aggaatgttg cccgtctggg gcgcgtatga
    13201 attaggtgtc agccccttct ggttaggctt aattgccatc gccgcctgtc ttggacacat
    13261 ctggcccgtt ttcttcggat ttaaaggagg aaaaggcgtt gctaccgctt ttggtgccat
    13321 cgcacccatt ggctgggatc tcaccggagt aatggcggga acctggttac tgaccgtgct
    13381 attgagcgga tactcgtcgc tgggagcgat tgtcagtgca ctgattgctc cgttttatgt
    13441 ctggtggttt aagccacaat tcaccttccc ggtttcgatg ctctcttgcc tgatcctgct
    13501 gcgtcatcat gacaacatcc agcgtctgtg gcgtcgtcag gagacaaaaa tctggacgaa
    13561 attcaaaaga aagcgcgaaa aggatcccga gtgatttctg gtggatctac atgacctgat
    13621 agccttcatc gggcttgccc agccgttgct ggcaccatgc cgccagaaat tccacgcaga
    13681 cacgtaattt catgctgcga tagagcggct cccggtaaac agcccagata ttggcgcttt
    13741 gtgcatactc cggcaatact tgcaccagtt tgccactctc cagaaacggc aacacatccc
    13801 actcggaacg cagcataatc cctttgccct ccagcgccca ttgcaacact atctcgccgc
    13861 tattggagga aagatgcccg ctaaccttca ccgatttttt ctcctgaccg ttccccaact
    13921 cccatattcc gtgggtcata tcgcgctctt tggtcaccag acagtcatga cgacttaatt
    13981 cctgtaatga ttgtggctgc ggatattttt gcagatactc aggtgctgcg cataatattc
    14041 ttttattttt cgttaacaga tgcgcaatat aataatcagg aatttcgtca ttaatacgaa
    14101 tatccagatc aatattatcc tgcactaaat caatttgccg atcgaacaat tcaaaatgca
    14161 cctgtaactc aggataattg cgcataagtt cggtaatggc tggcgcaata tggctgcgcc
    14221 caaaaccaaa actgcaacca atacgaatca tcccctccgg gcgcgttttg atttgcgtga
    14281 cgtcatcgac cagtcgctga tattgggtga ggatttccag cgcatgttcg taacaacgtt
    14341 ggccactttc agtcagcgcc acgccgcgag cggagcggtt cagcagcgtt gttgccagcg
    14401 tattttcaag gatttggatc cgcttggtga caaatgcagg cgtctgccca agcgtcgccg
    14461 ctgcggcgct aaaactcccg ctgtggacga tttctaccag tacctgcaag tctttggcta
    14521 aaggccagct attcagcatc gttaattatc cgcagttgtg ataagcgcag tgtatttcgc
    14581 aaaacattgc cattccctgc tctggcgcaa tattcattct tattttcgcg ttgattcacg
    14641 aattgttaat tacatattca cggtggcaaa aaatataaaa ccacattttt agtggtagtt
    14701 tgtggcggtg aatttttcca gacaaataca aaaactggag ttgccatgat gagcgaaagt
    14761 aataagcaac aggcagtgaa taagttgacg gagattgtcg ctaactttac cgccatgatt
    14821 tctacccgaa tgcctgatga cgtggtggat aaactaaaac agctaaagga tgccgaaacg
    14881 tcgtcgatgg ggaaaattat ctaccatacg atgttcgaca acatgcaaaa agcgattgac
    14941 ctgaatcgtc ctgcctgtca ggacaccggg gagattatgt tcttcgttaa agtcggttcc
    15001 cgcttcccac tgcttggcga gctgcaaagc atactcaaac aagccgtgga agaggcaacc
    15061 gtcaaagcgc cactacgtca caatgcggta gaaatttttg acgaagtaaa caccggcaaa
    15121 aataccggta gcggcgtacc gtgggtcacc tgggacatca tccccgacaa tgacgatgcg
    15181 gaaatcgaag tttacatggc aggcggcggc tgcacgctac ctggccgctc gaaagtgtta
    15241 atgccgtcag aaggctacga aggcgtggtg aaattcgtct tcgaaaatat ctccaccctc
    15301 gccgtaaacg cctgtccacc ggtactggtg ggcgtgggca tcgccacctc ggtggaaacc
    15361 gccgccgtac tctcgcgtaa agccattttg cgcccgattg gcagccgcca tcccaatcca
    15421 aaagcggcag aactggagct acgcctggaa gaaggactca accgtctggg gattggtcca
    15481 caagggctga ctggcaacag ttcagtgatg ggcgtacata tcgaatctgc cgcccgccat
    15541 ccgtcaacca tcggcgttgc tgtctctacc ggctgctggg cgcatcgtcg cggcacactg
    15601 ctggttcatg ccgatctcac ctttgaaaat ctgtctcaca cccggagcgc gttatgaaaa
    15661 agatcctgac aaccccgatc aaagctgaag atctgcaaga tattcgcgtc gacgatgtga
    15721 tctacctgac cggtacgctg gtgacctgcc gtgacgtttg tcaccgccgc ttgatcgaac
    15781 tgaaacgtcc gatcccttac gatctcaacg gcaaagcgat tttccacgct ggccccatcg
    15841 tgcgcaaaaa cggcgataaa tgggagatgg tttccgttgg cccgacaacc agtatgcgta
    15901 tggaaagttt tgaacgtgaa tttatcgagc agaccggcgt gaaactggtg gttggcaaag
    15961 gcggaatggg gccgctgacc gaagaaggct gccagaaatt caaggcgcta catgtgattt
    16021 tcccggcagg ctgcgcggtg ctggcggcaa cccaggtgga agagattgaa gaagtgcact
    16081 ggacagagct cggaatgccg gagtcgctgt gggtctgtcg ggtcaaagag ttcggcccgc
    16141 tgattgtctc tattgatacc cacggcaaca acctgatagc cgaaaacaaa aagctgttcg
    16201 ccgaacgccg cgatcccatc gtggaagaga tctgcgagca cgtccattac atcaaataac
    16261 cctcccggag aggctcgccc cctctccttt ttcgcaggca taacacgatg aaaccttcca
    16321 ctgaatggtg gcgatacctt gcgccgctgg cggtcatcgc cattattgct ctaattccgg
    16381 ttcccgcagg gctggagagt catacctggc tctactttgc cgtttttact ggcgtgatcg
    16441 ttggactgat cctcgaaccc gtgccgggtg ccgtggtggc gatggtgggt atatccatca
    16501 tcgccatact ctctccctgg ctgctgttca gcccggagca gctcgctcag ccaggcttta
    16561 aattcactgc aaaatccctc tcgtgggccg tttccggttt ttctaattcg gttatctggc
    16621 tgattttcgc cgcctttatg tttggcacag gctatgaaaa aaccgggctt ggacgccgca
    16681 ttgcgctgat tctggtgaaa aagatgggac atcgcacgct gtttctcggc tatgcggtga
    16741 tgttctccga gctgatcctc gcacctgtaa caccgtccaa ctcggcgcgt ggtgcgggga
    16801 ttatctatcc catcatccgt aacctgccac cgctctatca atcacaacca aacgacagca
    16861 gttcgcgcag cattggctcg tacatcatgt ggatggggat tgttgccgac tgcgtgacca
    16921 gcgccatttt cttgacggcg atggcaccaa acttgctgtt aattggactg atgaaaagcg
    16981 catctcacgc cacgctgagt tggggcgact ggttcctcgg gatgttgccg ctcagtattt
    17041 tgctggtgct gctggttccc tggctggctt atgtgctgta cccgccggta ctgaagtctg
    17101 gcgatcaggt gccgcgctgg gcagagacgg aactgcaggc aatgggcccg ctctgttcgc
    17161 gtgaaaaacg gatgctgggg ctgatagtag gcgcgctggt gctgtggatt ttcggcggtg
    17221 attatatcga tgccgcgatg gtcggttaca gcgtggtggc actgatgctg cttctgcgca
    17281 ttatcagttg ggacgacatt gtcagtaata aagcggcgtg gaacgttttc ttctggctgg
    17341 cctcgcttat caccctcgct accggactca acaacaccgg ttttattagc tggtttggca
    17401 aactgttagc aggcagctta agcggttatt cgccaacgat ggtgatggtg gcgttgattg
    17461 tggtgtttta tctgctgcgc tactttttcg ccagcgccac ggcgtatacc tccgccctcg
    17521 caccgatgat gattgccgct gcgctggcga tgccggaaat cccgctgccg gtgttctgcc
    17581 tgatggttgg cgcggcaatt ggtctgggga gcattcttac accatacgcc accggaccca
    17641 gtccgattta ctacggtagt ggttatctgc caacggcgga ttactggcga ctgggggcga
    17701 tttttgggct gatattcctc gtgttgctgg tgattaccgg cttactgtgg atgcccgtgg
    17761 tgttgcttta agtgaggacg gtgccggaaa ttgctctccg gcacccaata gttttacgca
    17821 gccggtaact ccgccagcgg ccagcgcgga cgcacgctaa cgccgagatc cgccgtcgcg
    17881 cctgctttaa accgcaccat tccggcatag gcgatcatcg cgccgttatc agtacaaaac
    17941 tccggacgcg cgtagaacac ttcgccgcgg cgttttttca tcatttcagc cagcttcgcc
    18001 cgtaacgtgc ggttagcact cacgccgccc gccatgacca gtcgcttaaa gcccgtctga
    18061 tccaacgctc gcttacactt aatcatcaac gtatcgacca ccgcatcttc aaaggcgcgg
    18121 gcgatatcag cacgcgtctg gtcgtcggtg ccgttgtcac gaatcgtgtt cgccgcaaag
    18181 gttttcagac cagaaaagct gaaatccagc cccggacggt cggtcatcgg acgcgggaaa
    18241 acaaagcgcc cggcagtacc ctgagccgcc attttcgaca gtaacggtcc gccaggataa
    18301 tccagcccca gcagcttcgc ggttttatca aacgcttcac cggcggcatc atcgatagac
    18361 tcgccgagca gctcgtactg accaatgcca gtcacgctga ttaactgcgt atgaccgccg
    18421 gacaccagca gcgcgacaaa cggaaattcc ggcgggttat cttccagcat cggcgctaac
    18481 agatgccctt ccatatggtg taccgggatt gccggaacgt tccaggcaaa cgccagagaa
    18541 cgccccacgg ttgcgccaac cagcagtgcg ccgactaatc cagggcctgc ggtataggcc
    18601 acagcatcaa tgtcttttgc cgttaagcca gactccttta gcgccgcctg gatcaacggt
    18661 acggttttgc gcacgtgatc gcgggaggcc agttcaggca cgacgccgcc atagtcagcg
    18721 tgcaatttca cctgactata caattggttg gctaacaaac ctttttcatc gtcgtaaatg
    18781 gcgatgccgg tttcatcgca ggaagtttca atacccagta cacgcatgac ttgttttacc
    18841 tcgctttatt accgcgcagt gtaggaccaa tgcgggttga tgtaaaactt tgttcgcccc
    18901 tggagaaagc ctcgtgtata ctcctcaccc ttataaaagt ccctttcaaa aaggccgcgg
    18961 tgctttacaa agcagcagca attgcagtaa aattccgcac cattttgaaa taagctggcg
    19021 ttgatgccag cggcaaaccg aattaatcaa aggtgagagg cacatgccgg taattaaagt
    19081 acgtgaaaac gagccgttcg acgtagctct gcgtcgcttc aagcgttcct gcgaaaaagc
    19141 aggtgttctg gcggaagttc gtcgtcgtga gttctatgaa aaaccgacta ccgaacgtaa
    19201 gcgcgctaaa gcttctgcag tgaaacgtca cgcgaagaaa ctggctcgcg aaaacgcacg
    19261 ccgcactcgt ctgtactaat tccccgagag cgttgctctc cgatcagacc gagttgtagt
    19321 tgtaaggccg tgcttccgaa aggaatgcgc ggcttatttt cgtttatgaa ttgctaaaaa
    19381 tcggggccta tggctggacg aatcccacgc gtattcatta atgatctgct ggcacgcact
    19441 gacatcgtcg atctgatcga tgcccgtgtg aagctgaaaa agcagggcaa gaatttccac
    19501 gcgtgttgtc cattccacaa cgagaaaacc ccgtcattca ccgttaacgg tgagaaacag
    19561 ttttaccact gctttggatg tggcgcgcac ggcaacgcga tcgacttcct gatgaactac
    19621 gacaagctcg agttcgttga aacggtcgaa gagctggcag caatgcacaa tcttgaagtg
    19681 ccatttgaag caggcagcgg ccccagccag atcgagcgcc atcagaggca aacgctttat
    19741 cagttgatgg acggtctgaa tacgttttac caacaatctt tacaacaacc tgttgccacg
    19801 tctgcgcgcc agtatctgga aaaacgcgga ttaagccacg aggttatcgc tcgctttgcg
    19861 attggttttg cgccccccgg ctgggacaac gtcctgaagc ggtttggcgg caatccagaa
    19921 aatcgccagt cattgattga tgcggggatg ttggtcacta acgatcaggg acgcagttac
    19981 gatcgtttcc gcgagcgggt gatgttcccc attcgcgata aacgcggtcg ggtgattggt
    20041 tttggcgggc gcgtgctggg caacgatacc cccaaatacc tgaactcgcc ggaaacagac
    20101 attttccata aaggccgcca gctttacggt ctttatgaag cgcagcagga taacgctgaa
    20161 cccaatcgtc tgcttgtggt cgaaggctat atggacgtgg tggcgctggc gcaatacggc
    20221 attaattacg ccgttgcgtc gttaggtacg tcaaccaccg ccgatcacat acaactgttg
    20281 ttccgcgcga ccaacaatgt catttgctgt tatgacggcg accgtgcagg ccgcgatgcc
    20341 gcatggcgag cgctggaaac ggcgctgcct tacatgacag acggccgtca gctacgcttt
    20401 atgtttttgc cggatggcga agaccctgac acgctggtac gaaaagaagg taaagaagcg
    20461 tttgaagcgc ggatggagca ggcgatgccg ctttccgcat ttctgtttaa cagcctgatg
    20521 ccgcaagttg atctgagtac ccctgacggg cgcgcacgtt tgagtacgct ggcactgcca
    20581 ttgatatcgc aagtgccggg cgaaacgctg cgaatatatc ttcgtcagga attaggcaac
    20641 aaattaggca tacttgatga cagccagctt gaacgattaa tgccaaaagc ggcagagagc
    20701 ggcgtttctc gccctgttcc gcagctaaaa cgcacgacca tgcgtatact tatagggttg
    20761 ctggtgcaaa atccagaatt agcgacgttg gtcccgccgc ttgagaatct ggatgaaaat
    20821 aagctccctg gacttggctt attcagagaa ctggtcaaca cttgtctctc ccagccaggt
    20881 ctgaccaccg ggcaactttt agagcactat cgtggtacaa ataatgctgc cacccttgaa
    20941 aaactgtcga tgtgggacga tatagcagat aagaatattg ctgagcaaac cttcaccgac
    21001 tcactcaacc atatgtttga ttcgctgctt gaactgcgcc aggaagagtt aatcgctcgt
    21061 gagcgcacgc atggtttaag caacgaagaa cgcctggagc tctggacatt aaaccaggag
    21121 ctggcgaaaa agtgatttaa cggcttaagt gccgaagagc gatcgggaag cccccgacag
    21181 ccgcactgag aggcagcggc aaatatataa gtacgccctc gtaattatcg ttggcggtaa
    21241 acaaccgttg gatttcagcg ttaacggctg aaggacatcg ggtcaatcgc ccaacaccaa
    21301 cctcatgaaa taagtgtgga taccgtctta tggagcaaaa cccgcagtca cagctgaaac
    21361 ttcttgtcac ccgtggtaag gagcaaggct atctgaccta tgccgaggtc aatgaccatc
    21421 tgccggaaga tatcgtcgat tcagatcaga tcgaagacat catccaaatg atcaacgaca
    21481 tgggcattca ggtgatggaa gaagcaccgg atgccgatga tctgatgctg gctgaaaaca
    21541 ccgcggacga agatgctgcc gaagccgccg cgcaggtgct ttccagcgtg gaatctgaaa
    21601 tcgggcgcac gactgacccg gtacgcatgt acatgcgtga aatgggcacc gttgaactgt
    21661 tgacccgcga aggcgaaatt gacatcgcta agcgtattga agacgggatc aaccaggttc
    21721 aatgctccgt tgctgaatat ccggaagcga tcacctatct gctggaacag tacgatcgtg
    21781 ttgaagcaga agaagcgcgt ctgtccgatc tgatcaccgg ctttgttgac ccgaacgcag
    21841 aagaagatct ggcacctacc gccactcacg tcggttctga gctttcccag gaagatctgg
    21901 acgatgacga agatgaagac gaagaagatg gcgatgacga cagcgccgat gatgacaaca
    21961 gcatcgaccc ggaactggct cgcgaaaaat ttgcggaact acgcgctcag tacgttgtaa
    22021 cgcgtgacac catcaaagcg aaaggtcgca gtcacgctgc cgctcaggaa gagatcctga
    22081 aactgtctga agtattcaaa cagttccgcc tggtgccgaa gcagtttgac tacctggtca
    22141 acagcatgcg cgtcatgatg gaccgcgttc gtacgcaaga acgtctgatc atgaagctct
    22201 gcgttgagca gtgcaaaatg ccgaagaaaa acttcattac cctgtttacc ggcaacgaaa
    22261 ccagcgatac ctggttcaac gcggcaattg cgatgaacaa gccgtggtcg gaaaaactgc
    22321 acgatgtctc tgaagaagtg catcgcgccc tgcagaaact gcagcagatt gaagaagaaa
    22381 ccggcctgac catcgagcag gttaaagata tcaaccgtcg tatgtccatc ggtgaagcga
    22441 aagcccgccg tgcgaagaaa gagatggttg aagcgaactt acgtctggtt atttctatcg
    22501 ccaagaaata caccaaccgt ggcttgcagt tccttgacct gattcaggaa ggcaacatcg
    22561 gtctgatgaa agcggttgat aaattcgaat accgccgtgg ttacaagttc tccacctacg
    22621 caacctggtg gatccgtcag gcgatcaccc gttctatcgc ggatcaggcg cgcaccatcc
    22681 gtattccggt gcatatgatt gagactatca acaaactcaa ccgtatttct cgccagatgc
    22741 tgcaagagat gggccgtgaa ccgacgccgg aagaactggc tgaacgtatg ctgatgccgg
    22801 aagacaagat ccgcaaagtg ctgaagatcg ccaaagagcc aatctccatg gaaacgccga
    22861 tcggtgatga tgaagattcg catctggggg atttcatcga ggataccacc cttgagctgc
    22921 cgctggattc tgcgaccacc gaaagcctgc gtgcggcaac gcacgacgtg ctggctggcc
    22981 tgaccgcgcg tgaagcgaaa gttctgcgta tgcgtttcgg tatcgatatg aacaccgacc
    23041 acacgctgga agaagtgggt aaacagttcg acgttacccg cgaacgtatc cgtcagatcg
    23101 aagcgaaggc gctgcgcaaa ctgcgtcacc cgagccgttc tgaagtgttg cgtagcttcc
    23161 tggacgatta atcggtaggc cggatcaggc gttacgccgc acccggcact agtccctctg
    23221 cacaaacgcc acctttt
//
LOCUS       DAZPDU010000055        22748 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZPDU010000055 DAZPDU010000000
VERSION     DAZPDU010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22748
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.055"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..470)
                     /gene="folK"
                     /locus_tag="ACHURS_001946"
     CDS             complement(<1..470)
                     /gene="folK"
                     /locus_tag="ACHURS_001946"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFR1237690.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDEL"
     gene            complement(467..1864)
                     /gene="pcnB"
                     /locus_tag="ACHURS_001947"
     CDS             complement(467..1864)
                     /gene="pcnB"
                     /locus_tag="ACHURS_001947"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFR1237691.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(1924..2820)
                     /gene="gluQRS"
                     /locus_tag="ACHURS_001948"
     CDS             complement(1924..2820)
                     /gene="gluQRS"
                     /locus_tag="ACHURS_001948"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFR1237692.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWTLTAVPESAIVNSTFSNASC"
     gene            complement(2887..3342)
                     /gene="dksA"
                     /locus_tag="ACHURS_001949"
     CDS             complement(2887..3342)
                     /gene="dksA"
                     /locus_tag="ACHURS_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFR1237693.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3520..4224)
                     /gene="sfsA"
                     /locus_tag="ACHURS_001950"
     CDS             complement(3520..4224)
                     /gene="sfsA"
                     /locus_tag="ACHURS_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFR1237694.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATRGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMNVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLSVTL"
     gene            complement(4239..4769)
                     /gene="thpR"
                     /locus_tag="ACHURS_001951"
     CDS             complement(4239..4769)
                     /gene="thpR"
                     /locus_tag="ACHURS_001951"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFR1237695.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4843..7272
                     /gene="hrpB"
                     /locus_tag="ACHURS_001952"
     CDS             4843..7272
                     /gene="hrpB"
                     /locus_tag="ACHURS_001952"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFR1237696.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERHYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFEPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLMAAKRL
                     LQMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPLMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLAALETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGGVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7469..10003
                     /gene="mrcB"
                     /locus_tag="ACHURS_001953"
     CDS             7469..10003
                     /gene="mrcB"
                     /locus_tag="ACHURS_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFR1237697.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDGELYE
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10223..12412
                     /gene="fhuA"
                     /locus_tag="ACHURS_001954"
     CDS             10223..12412
                     /gene="fhuA"
                     /locus_tag="ACHURS_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFR1237698.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMRQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIAGDGHTLNRGTVVDNERLQNFSVDTQLESKFATGDVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGSAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRISYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSAAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            12463..13260
                     /gene="fhuC"
                     /locus_tag="ACHURS_001955"
     CDS             12463..13260
                     /gene="fhuC"
                     /locus_tag="ACHURS_001955"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFR1237699.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13260..14150
                     /gene="fhuD"
                     /locus_tag="ACHURS_001956"
     CDS             13260..14150
                     /gene="fhuD"
                     /locus_tag="ACHURS_001956"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFR1237700.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14147..16129
                     /gene="fhuB"
                     /locus_tag="ACHURS_001957"
     CDS             14147..16129
                     /gene="fhuB"
                     /locus_tag="ACHURS_001957"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFR1237701.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLYRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16287..17567)
                     /gene="hemL"
                     /locus_tag="ACHURS_001958"
     CDS             complement(16287..17567)
                     /gene="hemL"
                     /locus_tag="ACHURS_001958"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFR1237702.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIIEPVAGNMNCVPPLPD
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17733..17780
                     /gene="yadX"
                     /locus_tag="ACHURS_001959"
     CDS             17733..17780
                     /gene="yadX"
                     /locus_tag="ACHURS_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFR1237703.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17792..19213
                     /gene="clcA"
                     /locus_tag="ACHURS_001960"
     CDS             17792..19213
                     /gene="clcA"
                     /locus_tag="ACHURS_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFR1237704.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19237..19302
                     /gene="yadW"
                     /locus_tag="ACHURS_001961"
     CDS             19237..19302
                     /gene="yadW"
                     /locus_tag="ACHURS_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFR1237705.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19295..19639
                     /gene="erpA"
                     /locus_tag="ACHURS_001962"
     CDS             19295..19639
                     /gene="erpA"
                     /locus_tag="ACHURS_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFR1237706.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19686..20309)
                     /locus_tag="ACHURS_001963"
     CDS             complement(19686..20309)
                     /locus_tag="ACHURS_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFR1237707.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEVGPLIAVCMVVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20347..21147)
                     /gene="btuF"
                     /locus_tag="ACHURS_001964"
     CDS             complement(20347..21147)
                     /gene="btuF"
                     /locus_tag="ACHURS_001964"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFR1237708.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21140..21838)
                     /gene="mtnN"
                     /locus_tag="ACHURS_001965"
     CDS             complement(21140..21838)
                     /gene="mtnN"
                     /locus_tag="ACHURS_001965"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFR1237709.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21922..>22748
                     /gene="dgt"
                     /locus_tag="ACHURS_001966"
     CDS             21922..>22748
                     /gene="dgt"
                     /locus_tag="ACHURS_001966"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFR1237710.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEQLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVA"
BASE COUNT         5144 a         5796 c         6362 g         5446 t
ORIGIN      
        1 cttaattcgt caaatgctct tgtatgtaag acttcacgca acgtctcccc atcaggaaac
       61 gccagctccg gtgcaatctc aaacagcggc cacagcataa atccacgatt cttcatatcg
      121 tagtgcggaa cggtcaggcg ttcggtattt atcacttcat taccaaacag catgatatcg
      181 agatccagcg tgcgtggtcc ccagcgttca gctttacgaa cacgaccctg ttgcagttct
      241 atgcgctgtg tatggttgag taactcttcc ggggcgagcg aagtttcaag cgccacggcg
      301 gcgttgaggt aatcgggttg atcttgcggc cccagcggtg gagtgcggta aaacgaagaa
      361 acggcaagaa tgtggctttc agggatatcg cctaatgctt tcagggcagc attgacctgc
      421 tccagcggag aggccagatt gctgcctatg gcaatatacg ccactgtcat gcggtaccct
      481 cacgacgcgg tgcgcgtttg cgtggacgac gagtacggcg acgcggtgac ggctcttcat
      541 ccaactcgtt aagcatccct ttttgatctg gtggcgcgga aacctggaac tcaccccacc
      601 atttcaccag acgctgcagt tcagcgttac gttcaacttc agctcgcaag gccaacaggt
      661 cataagccgc acggaactta ggatgctcca gcagtttcca tgcgcgttta ccctgacgtc
      721 gggacatacg caactgcaac tgccagatat cgcgggttaa cgtcgtcagg cgtttcggga
      781 ttgccagtga acggcaggct tcgtccagca cgtcgttcat cgccagcgcg aaagcgtcgt
      841 gataggtcag gccgctttcc tgggcgatct tctgtgccgt ctccagcagt gggtaccaga
      901 acatggcggc aaacaagaac gccgggttca cgcgcatatc gttatggata cgcgtatcgg
      961 tattcttcag cacctgttca atgatccgct ccatcgggct gtcgccattt tccgtgaagt
     1021 agcgggtaat ggtcgggaac agcggctgga acagatgata ttcacacaac agcttatagg
     1081 tttcgtaacc gtagcccgct tgtagcagtt taagcgattc ttcaaacagg cgtgccggtg
     1141 ggatatcgtt cagcagggta gcgaggcgag ggatcggttc tgcggtttcc gggctgatgc
     1201 gcatacccaa tttggcggca aaacgtaccg cgcgcagcat ccgtaccgga tcttcacggt
     1261 agcgcgtttc cgggttacca atcagacgga taacgccgtc cttcagatcc ttcataccgc
     1321 caacgtaatc acggacggta aaatccgcta cgctgtaata gaggctgttg atagtgaaat
     1381 cgcggcgctg ggcgtcttct tcgatggagc cgaaaatgtt gtcgcgcagc aacatgccgt
     1441 tttgcccgcg ttgggaggtc gtgcggtcgc tgacgttacc ttcgtggtgt ccacggaagg
     1501 tcgcaacttc gataatctcc gggccaaaca ttacatgagc cagacggaaa cggcgaccca
     1561 ccaggcggca gttacggaac agtttgcgca cctgctcagg cgtggcgtta gtggttacgt
     1621 caaaatcttt cggctttttg ccaagtaaca ggtcgcgcac gccgccgcca accagccagg
     1681 cttcgtatcc cgctttgttg agcctgtaca ttaccttcag ggcattttca ctgatatctt
     1741 tgcgggaaat agcatgctgc tcacgcggga tcaccgtcac ctgtggacgg gcgactgcct
     1801 gttcagcctc gctttcctcg cggcttagca ccttgcggca aaaattagcg actcgggtaa
     1861 aaatagtaca cctcggtagt gtcaaacatc attcaggaca aaaaaatagc ggctaatcat
     1921 agctcagcat gacgcatttg agaatgttga atttacaatt gccgactcgg gcacggcggt
     1981 cagcgtccag tttttgactg ctgactgaag gatttgttcg acgctgaaat cctgccagtg
     2041 tgcttctgcc tgctgaccca gaaattgaag tgccgcgatt agtaccggac gtggatcgcc
     2101 tttcggcaac gcaggcgcat gattctgctt ggaaagttta gcgccttgtg gattaagcgc
     2161 cagcggcaga tgaatgtaat ctggcacttt ccagccaaaa agctggtaca gcgagatttg
     2221 ccttactgtt ggttcaatca gatcagcccc acgcactatt tctgtaacgc cctggaaatg
     2281 atcatcaacc acaacagcca ggttgtaggc gaacaaccca tcacggcgat gaatgataaa
     2341 atcttcccgt gccagttttt cgtcggcgtg aataatgccg cgcagctggt cagtaaattg
     2401 cgtgaccgga tgctgctggc ggatacgcac tgcggcattg tctggtccat gatgcaacac
     2461 ccggcaatga ccgtcgtaaa taccgccaat gctttgaata cgcgcacgcg tacaggtgca
     2521 gtaataactt agtccttgtt catgtaacca ggcgagtgct tcacgatagg cgtcgtgacg
     2581 ttgcgattgc cagagaacat cgccgtccca gtgcagaccg taatgttcca gctggcgcag
     2641 gatagtttct gcggcaccgg gaacttcacg aggcgggtcg atatcttcta tgcgtaccag
     2701 ccagcgacct tgccgggcgc gagcctgcaa atagctgccg agcgcggcga tcagagagcc
     2761 aaaatgaagc tcgccggaag gagagggggc gaagcggcca atatactgtg tgtctgtcat
     2821 ctctttgaac aaaaaataag gcgggagcat ttcccgcctg tggtaaacgt gatggaacgg
     2881 ctgtaattag ccagccatct gtttttcgcg aatttcagcc agcgttttgc agtcgatgca
     2941 cagatcggct gtcgggcgcg cttccagacg gcgaatacca atttcaacac cgcaggattc
     3001 gcagtagccg aaatcttcgt cttccacttt tttcagcgtc ttctcgatct ttttgatcag
     3061 cttacgctcg cgatcgcggt tacgcagttc gaggctgaac tcttcttcct gggctgcacg
     3121 gtctaccggg tccgggaagt tggctgcttc atcctgcata tgtgtaacgg tgcgatcgac
     3181 ttcatccctg agttgattac gccatgcttc cagaatacga cggaagtgcg ccagctgggc
     3241 ttcattcata tactcttcgc ccggcttctc ctgatatggt tccaccccag cgatggcgag
     3301 aatactcagg gacgatgttt tacggttttg cccttcttgc atgttgcttc tccttaacac
     3361 gcactatcga tccccatgtt cgggggaaaa atgaggccgc tataaatagc agatgctttt
     3421 ccggatagca attatctaaa cgtaacactt gacaactgtg tgaggaaaag cgtatttgcg
     3481 cacgcgacca gaatgtaaat taaccagtta cttactttac tacaatgtaa ccgacagtga
     3541 ttttttaaga gccatgcctt cagcagaaat ttccgctttg taagccagaa tttctacccc
     3601 cctctgttga gcttctgaca atagttgcgc gtatttctca tcgatgtggc gcgcgggtga
     3661 aaaccgtgta atggctgaat gcagcacggc gaaaaagata accgcacgct ggccttcagc
     3721 cgctacgttc atcaactccc gaaggtgttt ctgacctcgt tcagtgaccg catcgggaaa
     3781 atatccctgt tcgttctccg ctaacgtaac cgatttcact tcaatatagc agtctggacg
     3841 cgaatccgcc tgcaacataa agtcaatacg gctgcgttct gcgccgtatt ttacttcgct
     3901 tttcagcgag ctatagcctg acagttctga aattgattca ttaaggatag cctctttcgt
     3961 caacctgtta gcccaaagcg tattgacgca aataaatgcg ccgctctggc tttgagttag
     4021 ttcccaggtg tgtgggtatt tccgtttggt gttgtctgaa gtcgaatacc agacggtatc
     4081 gccacgcgtt gcacaaccgg tcatcgcacc cgtattcggg cagtgtagcg ttaattcgcg
     4141 accatcgggt gtgatcacat cggctaaaaa acgtttgtaa cgctgaatta gcgtcgcgcg
     4201 ctgtagaggg ggagagaatt ccattgcgac aatccttgtt attgcgttag cgcccagcgt
     4261 tttagcggcg tgtagcgtgt gcgtccacgg gcaaacgagg aggcgtaaag ggtgaactcc
     4321 gtcaccgcat aagaccagtt aaaacctggc ggcgggattg tcaccgcctc gctggcgtcg
     4381 cgcaataagg taatatgtgg atgaaacgga cgattgcttt gaaaacaacc gctgcgggca
     4441 gcctgtgaac ggagcatatt cgccagctgg attaagccgc gtggcggctg acgcatccct
     4501 aaccacacca cacgcgaacg cagccattgt ccggcgtcat caagcgtgag tgtgaaacca
     4561 ggttgacgaa tccgtccggc taaaagagaa agcgccttct ctttctcggc gctcacttcg
     4621 cctaaaaatg ccagagtcag atgcaaatta tcggcggcga ccggacgtcc cgcctcaggt
     4681 gggaagtgtg tggcgcgcca gtggataatc tgttcgcgga tttctgcagg taagtcgata
     4741 gcaaagaaca gacgttgcgg ttcagacatg tgaggcactc ggttatgaat taccgcgatg
     4801 ctacaatgtg gcgcgaagaa tgttaaccct ctggagcgtt ttgtgtcgtc gttgcccgtt
     4861 gctgccgtct tacctgaatt acttaccgct ctcgattgtg cgccgcaggt attattaagt
     4921 gcgccgaccg gggccgggaa atcaacctgg ctgccgctgc aactgctggc gcatcccggc
     4981 attaacggga aaattatcct gctggagccg cgtcgtctgg cggcgcgtaa cgtcgcgcaa
     5041 cggctggcgg agctgcttaa cgaaaagcca ggcgataccg ttggctaccg gatgcgtgcg
     5101 caaaactgcg tcgggccaaa tacccgtctg gaagtagtta ccgaaggcgt gctgacgcgg
     5161 atgatccagc gtgacccgga gttgagcggt gttggattgg tgatccttga tgaatttcat
     5221 gagcgcagct tgcaggcgga tttggcgttg gcgctgttac tcgatgtgca acaaggtctg
     5281 cgtgatgacc ttaaactgct gattatgtcg gctacgctgg ataacgaccg cttgcagcaa
     5341 atgctgccag aagcgcccgt cgtcatctca gaagggcgct cgtttccggt tgaacgccat
     5401 tatttaccgc tgcccgcgca tcagcgtttt gacgaagccg ttgcggtagc caccgccgaa
     5461 atgctgcgtc aggaaagcgg atcattactg ttatttttac ctggcgtcgg agaaattcag
     5521 cgtgtgcagg aacaactggc ttcgcgcatc ggcagtgatg tgttgctctg cccgctgtat
     5581 ggcgcgttgt cgctgaacga tcagcgaaaa gcgatcctcc cggcaccgca agggatgcgc
     5641 aaagtggtgc tggcgaccaa tattgctgaa accagtttaa ccattgaagg tattcgtctg
     5701 gtggtggatt gtgcccagga gcgtgtggcg cgttttgagc cgcgcacggg gcttacgcga
     5761 ctgattactc aacgcgttag ccaggcatcc atgacgcagc gtgccgggcg cgccgggcgt
     5821 ctggagccgg gtatctgcct gcatttaatc gccaaagaac aagcagaacg cgctgccgcg
     5881 caaagtgaac cggagatctt acaaagcgat ctttccggtt tgctgatgga attactgcaa
     5941 tggggatgca gcgatccggc gcagatgagc tggctggatc aaccgccagt agtgaatcta
     6001 atggccgcga aacgtctgtt acaaatgctg ggggcgctgg acggtgaacg gcttagtgcg
     6061 caagggcaaa aaatggcagc gctgggtaac gatccgcgtt tagcggcaat gctggttagc
     6121 gcgaagaacg acgacgaagc tgctaccgcg gcaaaaattg ccgccattct cgaagagccg
     6181 ccactgatgg gcaatagtga cctgggcgtg gcgttttcgc gcaatcaacc agcctggcag
     6241 caacgtagtc agcaactgtt aaaacgctta aacgtacgcg gcggtgaggc agacagttcg
     6301 cttatcgcgc cgctacttgc cggggcgttt gccgatcgca ttgctcatcg ccgtgggcaa
     6361 gatggacgct atcaactggc aaacggcatg ggagcgatgc tcgatgccga cgacgcgcta
     6421 agccgccacg aatggttgat cgcaccgtta ttattgcagg gcagcgcctc gccggatgcg
     6481 cggattttac tggcgctacc ggtcgatatt gatgaattag tacaacgctg tccgcagctg
     6541 gtgcagcaat ctgacaccgt ggaatgggat gacgcgcaag gtacgctgaa agcctggcgt
     6601 cggctacaaa tcggtcagtt gacggtgaaa gtgcagccgc tggcgaaacc gtcagaagac
     6661 gagttgcatc aggcgatgct taatggcatc cgtgataaag gtttaagcgt gctcaactgg
     6721 acggcggaag cggaacagct acgcttgcgt ttgttatgcg ccgcaaagtg gttgccggaa
     6781 tatgactggc cagcggttga tgatgaaagt ttattggcag cgctggaaac gtggctgctg
     6841 ccacatatga caggcgtaca ttcactacgc ggcctgaaat cactggacat ttatcaggca
     6901 ctacgcggat tacttgattg gggaatgcag caacgtctgg atagtgaatt gcctgcgcat
     6961 tacactgtgc cgacgggaag ccggatcgcc attcgttatc atgaagataa cccgcccgca
     7021 ctggcggtga gaatgcagga gatgtttggt gaggccacta atccgactat cgcccagggg
     7081 ggcgtgccgc tggtgctgga gttgctttca cccgcccaaa ggccactaca aatcacacga
     7141 gatttgagcg ccttctggaa aggagcgtac cgtgaggtgc aaaaagagat gaaagggcgt
     7201 tatcccaaac acgtctggcc ggacgacccg gcaaacacgg caccgacgcg acggacgaaa
     7261 aagtattcgt aaggttggtt ttctccctct ccctgtggga gagggccggg gtgagggcat
     7321 cagcgtgcgc atttccccct caccctaacc ctctccctca agggagaggg gaccgatcga
     7381 gcacaaattt tgagagatat cttcttctgt cttgtaacag aagaacagaa aatcgggctt
     7441 ttgcgcctga atattgcgga gaaaaagcat ggccgggaat gaccgcgagc caattggacg
     7501 caaagggaaa ccgacgcgtc cggtcaaaca aaaggtaagc cgtcgtcgtt acgaagatga
     7561 cgatgattac gacgattatg atgactatga ggatgaagaa ccgatgccgc gcaaaggtaa
     7621 gggcaaaggc aaagggcgta agcctcgtgg caaacgcggc tggctatggc tgctgttaaa
     7681 actggctatc gtttttgccg tgctgatcgc catttacggc gtttatctcg atcaaaaaat
     7741 tcgtagccgt attgatggca aggtctggca actgcccgcg gcagtttatg gccgaatggt
     7801 caaccttgaa ccagatatga ccatcagcaa gaacgaaatg gtgaagctgc tggaggcaac
     7861 acagtatcgt caggtgtcga aaatgacccg tcctggcgaa tttaccgtgc aggccaacag
     7921 cattgagatg atccgccgtc cgtttgattt cccggacagc aaagaagggc aggtgcgtgc
     7981 gcgtctgacc tttgatggcg atcatctggc gacgatcgtc aatatggaga acaaccgtca
     8041 gttcggtttc ttccgtcttg atccgcgcct gatcaccatg atctcttcgc caaacggtga
     8101 gcagcgtctg tttgtgccgc gcagtggttt cccggacttg ctggtggata ctttgctggc
     8161 gacagaagac cgtcatttct acgagcatga tgggatcagt ctctactcca tcggacgtgc
     8221 ggtgctggca aacctgaccg ccggacgcac ggtgcaggga gcgagtacgc tgacgcaaca
     8281 gctggtgaaa aacctgttcc tctccagcga gcgttcttac tggcgtaaag cgaacgaagc
     8341 ttacatggcg ctgatcatgg acgcgcgtta cagcaaagac cgtattcttg agctgtatat
     8401 gaacgaggtg tatctcggtc agagcggcga caacgaaatc cgcggcttcc cgcttgcgag
     8461 cttgtattac tttggtcgcc cggtagaaga gctcagcctc gaccagcagg cgctgttagt
     8521 cggtatggtg aaaggggcgt ccatctacaa cccgtggcgt aacccgaaac tggcgctgga
     8581 gcgacgtaat ctggtgctgc gtttgctaca acaacagcag attatcgatg gtgaactgta
     8641 tgaaatgctc agcgcgcgtc ctctgggcgt acagccgcgc ggtggggtaa tctctccaca
     8701 gccagccttt atgcaactgg tgcgtcagga gctgcaggcg aaattaggcg ataaggtaaa
     8761 agatctctcc ggcgtgaaga tcttcactac ctttgactcg gtggcccagg acgcggcaga
     8821 aaaagccgcc gtggaaggca ttccggcact gaagaaacag cgtaagttga gcgatcttga
     8881 aactgcaatt gtggtcgtcg accgctttag tggtgaagtt cgtgcgatgg tgggaggttc
     8941 tgagccgcag tttgcgggct acaaccgtgc gatgcaggcg cgtcgttcga ttggttccct
     9001 tgcaaaacca gcgacttatc tgacggcttt aagccagccg aaaatctatc gcctgaacac
     9061 gtggattgcc gatgcgccaa tcgcgctgcg tcagccgaat ggccaggtct ggtcaccgca
     9121 gaacgatgac cgtcgttata gcgaaagcgg cagagtgatg ctggtggatg cgttgacccg
     9181 ttcgatgaac gtgccgacgg taaatctggg gatggcgctg gggctgcctg cagttacgga
     9241 gacctggatc aaactgggcg tgccgaaaga tcagctgcat ccggttccgg cgatgctgct
     9301 gggggcgttg aacttaacgc caatcgaagt ggcgcaggca ttccagacca tcgccagcgg
     9361 cggtaaccgt gcaccgcttt ctgcactgcg ttcggtaatc gcggaagatg gcaaggtgct
     9421 gtatcagagc ttcccgcagg cagaacgcgc tgttccggcg caggcggcgt atctgacact
     9481 atggaccatg cagcaggtgg tacaacgtgg tacgggtcgt caacttgggg cgaaataccc
     9541 gaacctgcat ctggcaggga aaacagggac taccaacaat aacgtagata cctggtttgc
     9601 gggcattgac ggcagcacgg tgaccatcac ctgggtaggc cgtgataaca accagccgac
     9661 caaactgtat ggtgccagcg gggcaatgtc tatttatcag cgttatctgg ctaaccagac
     9721 gccaacgccg ctgaatcttg ttccgccaga agatattgca gatatgggcg tggactacga
     9781 cggcaacttt gtttgcagcg gtggcatgcg tgtcttgccg gtctggacca gcgatccgca
     9841 atcgctgtgc cagcagagcg agatgcagca gcagccgtca ggcaatccgt ttgatcagtc
     9901 ttctcagccg cagcaacagc cgcaacagca acctgctcag caagagcaga aagacagcga
     9961 cggtgtagcc ggttggatca aggatatgtt tggtagtaat taacatctaa gcgtgaaata
    10021 ccggatggcg aattgccatc cggtaaaata acatcccacc taaagtatta accctttctt
    10081 ttcatctggt tgtttattaa cccttcagga acgctcagat tgcgtaccgc ttgcgaaccc
    10141 gccagcgttt cgaatattat cttatcttta taataatcat tctcgtttac gttatcattc
    10201 actttacatc agagatatac caatggcgcg ttccaaaact gctcagccaa aacactcact
    10261 gcgtaaaatc gcagttgtag tagccacagc ggttagcggc atgtctgttt atgcacaggc
    10321 agcggttgaa ccgaaagaag acactatcac cgttaccgct gcacctgcgc cgcaagaaag
    10381 cgcatggggg cctgctgcaa ctattgcggc gaagcactct gctactgcga ctaaaacgga
    10441 tacaccaatt gaaaaaacgc cgcagtctat ttcggttgtg acaaatgaag agatgcagat
    10501 gcgtcaattt cagtctgtaa aagaagcatt aggttataca cctggcgtta cggttagcag
    10561 tcgtggtgct tctaatacat atgattttgt tatcattcgt ggtttctcat ctgttggtct
    10621 gaatcaaaat aattaccttg atggactaaa acttcagggc aacttttata acgatgctgt
    10681 gattgatcct tacatgcttg agagggttga actgatgcgt gggccgacgt ctgttctcta
    10741 tggaaaaagt aatcctggtg ggattatttc tatggtgagt aagcgtccga ccactgaacc
    10801 cctgaaagaa attcagttta aaatggggac ggataatctg tttcagaccg gattcgattt
    10861 cagtgatgca ctggatgata acggtgagtt ctcttaccgt ttgacgggcc tcgctcgctc
    10921 aacaaacgaa cagcagaaaa actctgaatc tcagcgttat accattgctc catcattctc
    10981 atggcgtcca gacgacaaaa ccaattttac tttcctgtcc tatttccaga atgaaccaga
    11041 aacgggttat tacggttggt tgccgaaaga ggggaccgtt gagccattgc ctaatggtaa
    11101 gcgtctaccg actgacttca atgaaggtgc atcgaataat acatactccc gtaaccagaa
    11161 aatggtggga tatagttttg aacatggttt taatgacacc ttcaccgtgc gtcagaatct
    11221 gcgtttcagt gaaatgaaaa cctcacagaa aagtgtttat ggcacaggga ttgctggtga
    11281 tggtcatacc ctaaaccgcg ggacagtggt ggataatgag cgtctacaaa actttagcgt
    11341 tgatacccaa cttgaaagta agtttgctac aggtgacgtc gagcatactt tgctgacagg
    11401 ggtagacttc atgcggatgc gtaatgatat caatgccagc tttggatccg caccatccat
    11461 cgatctttat aacaaatatc accctgaata ctttgcattt ggtagcgcag agccatacca
    11521 gatgaatgaa agcaaacaaa caggtattta tgttcaggat caggcggaat ggaataaatg
    11581 ggtattcact ctggggggac gctacgattg gtctaagcaa gcgaccactg ttcgtgaaaa
    11641 ctcttatacg ccgactgaag gttatattga gcgcaatgat catcagttca cctggcgcgg
    11701 tggtgtaaat tacttattcg ataatggtat ttcaccttac tttagctata gccagtcctt
    11761 tgaaccgagt gctttcgatc tgtggagcaa cccgcgcatt tcctataagc catcgaaagg
    11821 tgaacagtat gaagctggcg taaaatatgt tccgaatgat atgccggtcg ttgttacggg
    11881 cgcagtctat caattgacga aaacaaataa cctgacagca gacccaacaa acccattagc
    11941 gcaagtccca gcaggtgaga ttcgcgctcg tggtgtggaa cttgaagcaa aagcggcgtt
    12001 aaatgccaat attaacttga cggcttctta tacctacacc gatgcggaat acaccaaaga
    12061 caccaatctc aaaggtaaaa ctccagaaca agtaccggag catatggcat ctctctgggg
    12121 ggattatacc ttcaatgaag ggccgctttc tggtttaaca ttgggaacag gtggtcgttt
    12181 tattggttcc agctatggtg atccggcaaa cacttttaaa gtgggtagcg cagctgtaat
    12241 ggatgctgtt gtaaaatatg atctggcacg ctttggtatg gcgggatcca gccttgctgt
    12301 gaacgtcaac aatttgctcg atcgtgagta tgttgccagt tgcttccaga cttatggctg
    12361 cttctggggc gcagaacgtc aggtcgttgc aaccgcaacc ttccgtttct aatttctctt
    12421 ttggggcacg gatttccgtg cccatttcac aagttggctg ttatgcagga atacacgaat
    12481 cattccgata ccacttttgc actgcgtaat atctcctttc gtgtgcccgg gcgcacgctt
    12541 ttgcatccgc tgtcgttaac ctttcctgcc gggaaagtga ccggtctgat tggtcacaac
    12601 ggttctggta aatccactct gctcaaaatg cttggtcgtc atcagcctcc gtcagaaggg
    12661 gagattcttc ttgatgccca gccgctggaa agctggagca gcaaagcgtt tgcccgcaaa
    12721 gtggcttatt tgccgcagca gcttcctccg gcagaaggga tgaccgtgcg tgaactggtg
    12781 gcgattggtc gttacccgtg gcatggcgcg ctggggcgct ttggtgcggc agatcgcgaa
    12841 aaggtcgagg aagctatctc gctggttggc ttaaaaccgc tggcgcatcg gctggtcgat
    12901 agtctctctg gcggcgaacg tcagcgggcg tggatcgcca tgctggtggc gcaggatagc
    12961 cgttgtctgt tgctcgacga accgacttcg gcgctggata tcgcccacca ggttgatgtg
    13021 ctggcgctgg tgcaccgttt aagtcaggaa cgtggcctga cggtcattgc cgtgttgcac
    13081 gatatcaata tggcggcacg ctactgtgat tatctggtcg ccctgcgcag tggtgaaatg
    13141 attgctcagg gaacgcctgc ggaaattatg cgcggcgaaa ccctcgaaat gatttatggc
    13201 atcccgatgg gtattttgcc gcatccggcg ggtgctgcac ctgtgagttt tgtttattga
    13261 tgagcggctt acctcttatt tcgcgccgtc gactgttaac ggcgatggcg ctttctccgt
    13321 tgttatggca gatgaatacc gcccacgcgg cggctattga ccccaatcgt attgtggcgc
    13381 tggagtggtt gccggtggaa ttactgctgg cgctcggcat cgtgccttac ggcgtggcgg
    13441 ataccatcaa ctatcgcctg tgggtcagcg aaccaccatt gccggactca gtgattgacg
    13501 tcggtttgcg tacagaacct aaccttgaac tgctgaccga aatgaaaccg tcgtttatgg
    13561 tctggtcggc aggatatggc ccttcatcag aaatgctggc acgtattgcg ccgggtcgcg
    13621 gatttaactt cagtgacggc aaacagccgt tggcgatggc gcgtaaatcg ctgacggaaa
    13681 tggcagattt acttaacctg caaagcgcag cggaaacgca tttagcgcaa tacgaagact
    13741 ttatccgcag catgaaaccc cgctttgtga agcgtggcgc acgcccgtta ctgctgacga
    13801 cgcttatcga tccgcgccat atgctggtct tcggtccaaa cagcttgttc caggaaattc
    13861 tcgatgagta cggcatccca aatgcctggc aaggggaaac caacttctgg ggcagtaccg
    13921 ccgtcagtat cgatcgtctg gcagcgtata aagacgttga tgtgctctgt tttgatcacg
    13981 acaacagcaa agacatggat gcgctaatgg caaccccgct gtggcaggcc atgccgtttg
    14041 tccgcgccgg acgctttcag cgcgtacctg cagtctggtt ttatggtgcg acactctcgg
    14101 caatgcactt tgtgcgcgtt ctggataacg ccatcggagg taaagcgtga gtaaacgaat
    14161 tgcgcttttt ccggcgttat tgctggcgct gttagtgatt gtcgctacgg cgctcacctg
    14221 gatgaacttc tcgcaggcgc tgccgcgtag ccagtgggcg caggctgcct ggtcgccaga
    14281 catcgatgtc atcgagcaga tgatttttca ctacagcttg ttgccgcgtc tggcgatttc
    14341 gctgctggtg ggcgcgggcc tggggctggt gggcgtgctg tttcagcaag tgctgcgtaa
    14401 cccgctggcg gagccgacga cgcttggcgt tgctacaggc gcgcaactgg gaattaccgt
    14461 caccacactt tgggcgatcc caggtgctat ggcgagccag tttgccgcac tggtgggcgc
    14521 ttgtgtcgtt ggcttaatcg tctttggcgt cgcgtggggg aaacggcttt cgccggttac
    14581 gcttattctc gcggggctgg tggtgagcct ttattgcggg gcaattaatc agctactggt
    14641 tatcttccat cacgaccagc tgcaaagcat gttcctgtgg agcaccggaa cgctgacgca
    14701 aaccgactgg ggcggcgttg agcgtttatg gccgcagttg ctgggcggcg tgatgttgac
    14761 gttattgcta cttcgcccgt taaccctgat ggggcttgat gatggcgtgg cgcgcaatct
    14821 cgggctggcc ttgtcgctcg cgcgtctggc ggcgctgtcg ctggcgatag tcatcagtgc
    14881 gctgctggtg aatgcggtgg ggattatcgg ctttatcggt ttgttcgcgc cgctgctggc
    14941 gaaaatgctg ggggcgcggc gtttgttgcc acggttgatg ctggcgtcgc tgattggggc
    15001 gctgatcctc tggctttccg atcaaatcat cctctggctg actcgcgtgt ggatggaagt
    15061 gtccaccggt tcggtcactg cgttgatcgg tgcgccgctg ctactgtggc tgttgccgcg
    15121 tttacgcagc attagcgcgc cggatatgaa ggtcaacgat cgtgtcgcgg ctgaacgcca
    15181 acatgtgctg gcgtttgccc tcgcgggcgg cgtgctgctg ttgatggctg tggtggtggc
    15241 gctgtcgttt ggtcgtgatg cgcacggctg gacgtgggcg agcggggcgt tgctcgagga
    15301 tttgatgccc tggcgctggc cgcgaattat ggcggcgctg tttgcgggcg tcatgctggc
    15361 ggtggcgggc tgtattattc agcggctgac cggaaacccg atggcaagcc cggaagtgct
    15421 ggggattagc tctggcgcgg cgtttggcgt ggtgttaatg ctgtttctgg tgccgggtaa
    15481 tgcctttggc tggctgttac ccgcgggcag tatcggggcg gcggtgacgc tgttgatcat
    15541 tatgatcgcc gccggccgcg gtggattttc cccacaccgt atgttactgg cggggatggc
    15601 gttaagtacc gcattcacca tgcttttgat gatgttgcag gcgagcggtg acccgcgaat
    15661 ggcgcaagtg ctgacctgga tttccggttc gacctacaac gcgaccgatg cgcaggtctg
    15721 gcgtaccgga attgtgatgg tgattttgct ggcgattacc ccgctgtacc gccgctggct
    15781 gaccatttta ccgctgggtg gtgataccgc acgagccgta ggaatggcgc tgacgccgac
    15841 gcgaattgcg ctgctgctgt tagcggcttg cctgacggcg accgcgacga tgactattgg
    15901 accgttgagt tttgttggtt taatggcacc gcatattgcg cggatgatgg gctttcgacg
    15961 gacgatgcca cacatcgtaa tttcggcgct ggtgggtggt ttactgctgg tattcgctga
    16021 ctggtgtggg cggatggtgc tgtttccatt ccagatcccg gcggggctgc tgtcgacctt
    16081 tatcggcgcg ccatatttta tctatttgct gagaaagcag agccgttaat ttttccatat
    16141 aacggccctg tacgcctgga cggataaagc gtttacgcga caacggatgc ccgatgcgac
    16201 gctggcgcgt cttatcgggc ctacaaacgg ccccgaatcg taggtcggat aaggcgttca
    16261 cgccgcatcc gacaaaccat gctggatcac aacttcgcaa acacccgacg tgcagcatcg
    16321 atggtgttat tgatatcttc catgctgtgc gccacggaca taaagcccgc ttcaaacgct
    16381 gacggtgcca ggtaaacacc ttcgtccagc atcatatgga agaaacgctt aaagcgttcc
    16441 acgtcacagg ccatcacatc ctgatagcac gtcacggact cggcgtcggt aaagaaaata
    16501 ccgaacatgc cgccaacgtg gttaacgacc agcggaattc cggcttcttc tgccgcttcc
    16561 agcagacctt ctgccagacg tgttgtcagc tcatccaacg tttcgtgaac gcccggttgc
    16621 gcgacttcat tcagacaggc gaaaccggct gccatcgcaa ttgggttacc ggaaagcgta
    16681 cccgcctgat agaccggacc cgtcggggcc agcgcatcca ttacatcacg acgaccaccg
    16741 aatgcgccta ccggcattcc accgccgatg attttgccca ggcaggtgag atccggttcc
    16801 acaccgtaat aatcctgtgc gccagccagt gccacgcgga agccggtcat tacttcatcg
    16861 atgatcagca atgcgccaaa ttcgtcgcac agcgcacgca gacctggcag gaagtctggc
    16921 agcggtggaa cacagttcat attgcctgcc accggctcga tgataataca ggcaatctct
    16981 tgcgggtatt gctcaaacgc ggcgcgtaca gaagccagat cgttataagt acaggttaag
    17041 gtatgtttgg cgaaatctgc cggaacgccc ggcgagtttg gctggcctaa cgtgagtgcg
    17101 ccagaaccgg ctttcaccag caggcagtca gcgtgaccgt ggtaacaccc ttcaaattta
    17161 atgattttgt cgcgaccggt aaaaccacgg gccagacgga tggcgctcat cgtcgcctcg
    17221 gtgccggagt tcaccatgcg caccatatcc atggtcggga ccaattcggt caccagttgc
    17281 gccattttca cttccatttc ggttggtgca ccaaagctta aaccacgctc ggcggcttca
    17341 atcacggcat tgcggattgc cggatggtta tggcccagca ccatcggccc ccaggaaccg
    17401 acataatcga tataggcttt gccatcaaca tcgtacagat aagcgccgtc cgctttttcg
    17461 ataaacagtg gagtgccgcc cacgccagta aaggcgcgaa caggggagtt cacaccgcca
    17521 gggatcagct cgcgcgctgc gctgtaaaga ttttcagact tactcatgga gggttcctga
    17581 ttcgtagaaa aagtgaatgg ctgctattct atgttattca taacaagtta aatactcgtc
    17641 aaacatcagg ctgcttgtac tggtgcaatc ctgatttctt agagtataaa agttttgtgc
    17701 atttgaaaca ttacgctttg caaaggattt tcatggaacg tgcgagtaaa atgccgtcat
    17761 cttatttgta tgaccaataa gtgatcattg gatgaaaact gatactccct ctttagaaac
    17821 accgcaggcc gcgcgcctgc gacgcagaca actgattcgc caacttcttg agcgcgataa
    17881 aaccccgtta gccattttgt ttatggcggc agtcgtcggc acgcttgttg ggctggcagc
    17941 ggttgctttt gacaaaggtg tcgcctggtt gcagaaccaa cgtatggggg cgctggtaca
    18001 tactgctgat aattatccgc ttctgttaac cgtcgctttt ctctgttcgg cggtgctggc
    18061 gatgtttggc tactttttgg tgcgcaaata cgcgccggaa gcaggtggtt cggggatccc
    18121 ggaaattgaa ggggcgctgg aagatcaacg tcccgttcgc tggtggcgtg tattgccggt
    18181 gaagttcttt ggcgggctgg ggacactcgg cggaggcatg gtgttggggc gcgaagggcc
    18241 aaccgtgcag atcggcggta acattggccg tatggtgctt gatattttcc gcctgaaagg
    18301 tgacgaagct cgccatacgc tgctggcaac cggtgctgct gcggggctgg ctgcggcctt
    18361 taacgcgccg ctggcgggta ttttgtttat tatcgaagag atgcgtccgc agtttcgcta
    18421 tacgttaatt tcgataaaag cggtatttat tggcgtcatt atgtcgacca ttatgtaccg
    18481 gatttttaat catgaagttg cgttgattga cgtcggtaaa ctttctgatg cgccgcttaa
    18541 tacgctgtgg ctttatctga tcctcggtat tatttttggc attttcggcc ctatttttaa
    18601 taaatgggtg ctggggatgc aggatttgct gcaccgtgtg cacggcggca atattaccaa
    18661 atgggtactg atgggcggtg cgattggcgg tctgtgtgga ttgctggggt ttgtggcacc
    18721 agcaacgtcg ggcggcggtt ttaacctgat tcctatcgct accgcgggga atttcagcat
    18781 gggaatgctg gtgtttatct tcgtcgctcg ggtcattacc accttactct gcttctcttc
    18841 cggcgcgccg ggcggtattt ttgccccgat gctggcgctg ggtactgtgc tgggaaccgc
    18901 tttcggaatg gttgccgttg agctgtttcc gcaatatcac cttgaggcgg ggacgtttgc
    18961 tattgccgga atgggggcat tactggcggc atctattcgc gcgccgttaa cggggatcat
    19021 tctggttctg gagatgaccg ataactacca gctcattttg ccaatgatta ttaccggtct
    19081 tggcgcaaca ctattagcgc aatttaccgg cgggaaaccg ctatactcgg cgattcttgc
    19141 gcgcacgctg gcaaaacagg aagctgagca actggcgcga agcaaggccg catcagccag
    19201 cgagaatact tgaacgaaat accagggtat tagataatgg cgattattat tgggttagaa
    19261 tttgcccaat tgccgatgtc gtttggagca aaatatgagt gatgacgtag cactgccgct
    19321 ggagtttacc gacgcagcag ccaacaaagt taaaagcctg atcgctgacg aagataaccc
    19381 gaatctgaaa ttacgcgtgt atatcacagg tggcggttgc agcggcttcc agtatggttt
    19441 cacctttgat gatcaggtga acgaaggcga tatgaccatc gaaaaacagg gcgttggctt
    19501 ggtggttgat ccgatgagcc tgcaatatct ggtcggcggt tccgttgact ataccgaagg
    19561 actggaaggt tctcgtttca tcgtgaccaa cccgaacgcg aaaagcacct gcggttgcgg
    19621 ttcttccttt agcatctaat ctgttgttca tgtgccggat aaactatccg gcgcatcttc
    19681 tctcttcaac gcccattctc atccagcgca aacgtcggta gcttaagatg ccaacgaata
    19741 gccgccagcc gaatcaatag cgtcacgacc atgcccatca tactggctgt ttccagtggt
    19801 acggaaaatg tgtaataagc cgtagcgtgg acaataccgc cgataataca ggcagttgcg
    19861 tagatttctg tacgtaaaat catggggatc tcgcgggcca gtacatcacg aatgatcccg
    19921 ccgccaacgc cagtaatgac gaccatacaa accgcgatta acggaccgac ttccgcatta
    19981 aaggctttat tcacgccaat gccgacaaac accgccagac caacggcgtc cagcaccggc
    20041 aacatccatt ttggtaagcg tcttggctgg cgcaccagca cgatggtcag catgctggtt
    20101 accattgcaa cgaccagatc ggtgggatct ttcacccaaa ataccgggcc gtgatccagc
    20161 gccatgtcgc gaattgtccc gccgcctaca gcggtaacca cgcccagtac cagaacacca
    20221 aaagggtcca tacgcaattt tccggctaac aaaacgccgg agatggcaaa taccgctgtg
    20281 ccgactatat ccagccaata gacgagcatt gttcaatccc cactgagcac ctaaaaaggt
    20341 caggcgctaa tctacctgtg aaagcgcatt acagagctgt tgtgcagcga ggataatacg
    20401 tgggcttgca cgttcaaacc agtcactcgt gagaggaata acgggaattt tgagctgttc
    20461 accccagtat tgtttgattt taggaatttg gtccggtccg cctgtaatga caatcgcctg
    20521 tggcgagcgt gctaacacct gttcgcggct aacttgcggc cagggaaccc ggctgtcttt
    20581 aaagatgttt tctccgccac aaacttcgag tacctggttt tgaatcgact cttttccact
    20641 ggtaaatggc ggattaatgc cgaattgcag aaaaacacgt tttttaggtt tatcagcata
    20701 ttgcgctttc aattgcgcgt actgatccag cagggattgc gcggcttgtt cggccttatc
    20761 cggttgcgga ctccaggggg ccaattgacg taacgcattg gcaatttgtt caatgcttgt
    20821 cgcatcgacc cacatcactt ttattcccag cgaagccagc tggtcaacct gccgctcggc
    20881 attacctcca cgccaggcaa tcaccagatc gggtttcagc gcgacaatgc gttccagatt
    20941 catcccctgc caggtggaaa cctgctcaat cttttgcgct tgtggaggat agtcggaata
    21001 gctgctgacc ccaaccggcg tgatcccggc ggcaaaggca agttcagtgt tggcgggaga
    21061 aagcgtgatg acgcgcggcg cggcgttgag ccacagtggc gcaagaaaag acagggcgac
    21121 cagcgccctg aacagtgact tagccatgtg ccagtttctg caccagtgac tcaaccatca
    21181 ggctggactg tttagcggca acagccagga actcatcgaa gctaagatga gactgttgat
    21241 cggccacgtc ggagatggcg cgtacgacaa caaacgggac gttgaaattg tggcagacat
    21301 gggcgattgc cgtcgcttcc atctctacag caatggcctg tgggaagttg tggcggattt
    21361 tcgccagacc aacagaaccg ttgatgaaag cgtcgccgct aacaatcagg ccacgtacag
    21421 cgttaagatt cagttcggca atgcaggcct cagcggcagc gatcagttta tcgtcagctt
    21481 taaagcctgc cggacagcct ggtaactgac cgtattcata accaaatgcc gtgacatccg
    21541 cgtcgtgata acgcgcttcg tccgagacaa cgatatcgcc cactttcagc gttggtgcca
    21601 ggccaccggc agaaccggtg ttaataatca catctggctt gcagtgttcc aacagcagag
    21661 tggcacccag cgccgcagcg actttaccga tgcccgattt cagaagcgca acctcggttc
    21721 cattcagttg gccggtatag atttcgcaac cgccgagact gatagtttga cggttttcga
    21781 ttttgtcacg cagcagcgta acttcttctt ccattgcacc aatgatgccg attttcatag
    21841 atttactcgc gataagcccg atttgaaagc atagtttacc atgcgcttac ggggaagcgt
    21901 atttctcacg cgggagagga catggcacag attgatttcc gaaaaaaaat aaactggcat
    21961 cgtcgttacc gttcaccgca gggcgttaaa accgaacatg agatcctgcg gatcttcgag
    22021 agcgatcgcg ggcgtatcat caactctccg gcaattcgtc gtctgcaaca aaagacccag
    22081 gtttttccac tggagcgcaa tgccgccgtg cgcacgcgtc ttacccactc gatggaagtc
    22141 cagcaggtgg gacgctacat cgccaaagaa attttaagcc gtctgaaaga gcttaaattg
    22201 ctggaagcat acggcctgga tgaactgacc ggcccctttg aaagcattgt tgagatgtca
    22261 tgcctgatgc acgatatcgg caatccgccg tttggtcatt ttggcgaagc agcgataaat
    22321 gactggtttc gccaacgttt gcacccggaa gatgccgaaa gccagcctct gactgacgat
    22381 cgctgcagcg tggcggcact acgtttacgg gacggggaag aacagcttaa cgagctgcgg
    22441 cgcaagattc gtcaggactt atgtcatttt gaggggaatg cacaaggcat tcgtctggtg
    22501 catacattga tgcggatgaa tctcacttgg gcacaggttg gcggtatttt aaaatatacc
    22561 cgtccggcgt ggtggcgtgg cgaaacgcct gagacacatc actatttaat gaaaaagccg
    22621 ggttattatc tttctgaaga agcctatatt gcccggttgc gtaaagaact taatttggcg
    22681 ctttacagtc gttttccatt aacgtggatt atggaagctg ccgacgacat ctcctattgt
    22741 gtggcaga
//
LOCUS       DAZPDU010000056        22685 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZPDU010000056 DAZPDU010000000
VERSION     DAZPDU010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22685
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.056"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..223)
                     /locus_tag="ACHURS_001967"
     CDS             complement(<1..223)
                     /locus_tag="ACHURS_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFR1237711.1"
                     /translation="MLAKDRTNLKIEEIRMHKHHEIHRVKPLMPASCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYESYIANYPEAG"
     gene            complement(233..784)
                     /gene="ybcL"
                     /locus_tag="ACHURS_001968"
     CDS             complement(233..784)
                     /gene="ybcL"
                     /locus_tag="ACHURS_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HFR1237712.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGV
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKIWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEIIPVYEVK"
     gene            complement(953..1285)
                     /gene="emrE"
                     /locus_tag="ACHURS_001969"
     CDS             complement(953..1285)
                     /gene="emrE"
                     /locus_tag="ACHURS_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFR1237713.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            1466..1594
                     /locus_tag="ACHURS_001970"
                     /pseudo
     CDS             1466..1594
                     /locus_tag="ACHURS_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003844207.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(1619..1933)
                     /gene="fliE"
                     /locus_tag="ACHURS_001971"
     CDS             complement(1619..1933)
                     /gene="fliE"
                     /locus_tag="ACHURS_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFR1237714.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            2149..3807
                     /gene="fliF"
                     /locus_tag="ACHURS_001972"
     CDS             2149..3807
                     /gene="fliF"
                     /locus_tag="ACHURS_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFR1237715.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYCFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            3800..4795
                     /gene="fliG"
                     /locus_tag="ACHURS_001973"
     CDS             3800..4795
                     /gene="fliG"
                     /locus_tag="ACHURS_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFR1237716.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTYVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            4788..5474
                     /gene="fliH"
                     /locus_tag="ACHURS_001974"
     CDS             4788..5474
                     /gene="fliH"
                     /locus_tag="ACHURS_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFR1237717.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            5474..6847
                     /gene="fliI"
                     /locus_tag="ACHURS_001975"
     CDS             5474..6847
                     /gene="fliI"
                     /locus_tag="ACHURS_001975"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFR1237718.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            6866..7309
                     /gene="fliJ"
                     /locus_tag="ACHURS_001976"
     CDS             6866..7309
                     /gene="fliJ"
                     /locus_tag="ACHURS_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFR1237719.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            7306..8433
                     /locus_tag="ACHURS_001977"
     CDS             7306..8433
                     /locus_tag="ACHURS_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFR1237720.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALASETTTD
                     KAAPQVLVATDKPTTKGELLISDIVSDAQQADLLIPVDETLPVINVEQSTSTPLTTAH
                     TMTLAAVADKNTTKDEKADDLNEDLTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLLTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEEDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            8538..9002
                     /gene="fliL"
                     /locus_tag="ACHURS_001978"
     CDS             8538..9002
                     /gene="fliL"
                     /locus_tag="ACHURS_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFR1237721.1"
                     /translation="MTDYAISKKSKRSLWLPILVFITLAACASAGYSYWHSHQVAAED
                     KAQHRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVMYTAFILR"
     gene            9007..10011
                     /gene="fliM"
                     /locus_tag="ACHURS_001979"
     CDS             9007..10011
                     /gene="fliM"
                     /locus_tag="ACHURS_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFR1237722.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGNGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDGIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            10008..10421
                     /gene="fliN"
                     /locus_tag="ACHURS_001980"
     CDS             10008..10421
                     /gene="fliN"
                     /locus_tag="ACHURS_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFR1237723.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            10424..10789
                     /gene="fliO"
                     /locus_tag="ACHURS_001981"
     CDS             10424..10789
                     /gene="fliO"
                     /locus_tag="ACHURS_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFR1237724.1"
                     /translation="MNNHATVQSSTPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            10789..11526
                     /gene="fliP"
                     /locus_tag="ACHURS_001982"
     CDS             10789..11526
                     /gene="fliP"
                     /locus_tag="ACHURS_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFR1237725.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     SVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            11536..11805
                     /gene="fliQ"
                     /locus_tag="ACHURS_001983"
     CDS             11536..11805
                     /gene="fliQ"
                     /locus_tag="ACHURS_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFR1237726.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            11814..12599
                     /gene="fliR"
                     /locus_tag="ACHURS_001984"
     CDS             11814..12599
                     /gene="fliR"
                     /locus_tag="ACHURS_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFR1237727.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFVIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            12884..13507
                     /gene="rcsA"
                     /locus_tag="ACHURS_001985"
     CDS             12884..13507
                     /gene="rcsA"
                     /locus_tag="ACHURS_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFR1237728.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(13551..13739)
                     /gene="dsrB"
                     /locus_tag="ACHURS_001986"
     CDS             complement(13551..13739)
                     /gene="dsrB"
                     /locus_tag="ACHURS_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFR1237729.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            13902..14129
                     /gene="yodD"
                     /locus_tag="ACHURS_001987"
     CDS             13902..14129
                     /gene="yodD"
                     /locus_tag="ACHURS_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFR1237730.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            14427..15242
                     /locus_tag="ACHURS_001988"
     CDS             14427..15242
                     /locus_tag="ACHURS_001988"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFR1237731.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPTALWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPTRVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(15239..16933)
                     /gene="dgcQ"
                     /locus_tag="ACHURS_001989"
     CDS             complement(15239..16933)
                     /gene="dgcQ"
                     /locus_tag="ACHURS_001989"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFR1237732.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLSVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNTSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVCASDNA"
     gene            complement(17104..17286)
                     /locus_tag="ACHURS_001990"
     CDS             complement(17104..17286)
                     /locus_tag="ACHURS_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFR1237733.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(17365..18282)
                     /locus_tag="ACHURS_001991"
     CDS             complement(17365..18282)
                     /locus_tag="ACHURS_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFR1237734.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            18455..19376
                     /gene="yedA"
                     /locus_tag="ACHURS_001992"
                     /pseudo
     CDS             18455..19376
                     /gene="yedA"
                     /locus_tag="ACHURS_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
     gene            complement(19365..19835)
                     /gene="vsr"
                     /locus_tag="ACHURS_001993"
     CDS             complement(19365..19835)
                     /gene="vsr"
                     /locus_tag="ACHURS_001993"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFR1237735.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGSPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(19816..21234)
                     /gene="dcm"
                     /locus_tag="ACHURS_001994"
     CDS             complement(19816..21234)
                     /gene="dcm"
                     /locus_tag="ACHURS_001994"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFR1237736.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPSAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(21301..21957)
                     /locus_tag="ACHURS_001995"
     CDS             complement(21301..21957)
                     /locus_tag="ACHURS_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFR1237737.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAQKLAADDDVDMLVILTACYF
                     HDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCHAIAAHSFSAQIAP
                     LTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQHRPLDDKRYALDH
                     FQTKLLKLPQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGENEGVDHKVIDAFSPAG
                     "
     gene            complement(21997..22278)
                     /gene="drpB"
                     /locus_tag="ACHURS_001996"
     CDS             complement(21997..22278)
                     /gene="drpB"
                     /locus_tag="ACHURS_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFR1237738.1"
                     /translation="MEERLSRSPGGKLALWAFYTWCGYFVWAMARYIWVMSRIPDAPV
                     SGFESDLGSTAGKWLGALVGFLFMALVGALLGSIAWYTRPRPARSRRYE"
BASE COUNT         5566 a         5951 c         5723 g         5445 t
ORIGIN      
        1 gacctgcttc tgggtaattc gcaatataag attcatatcc acagggaaat aatattatct
       61 gattattatc aacagttaga ctatgcgtct cccaattgat aacttttttt ccctgacgga
      121 tacgacatga agctggcata agaggcttaa ccctgtgaat ctcatgatgt ttatgcatcc
      181 gtatttcttc aatctttagg ttagtcctat cttttgccag catactctta tcctacttta
      241 cctcataaac gggtattatc tcagcggttg caattttatt ggcattaagc atataaccaa
      301 ctaacgctcc gctggaatta gaatccacag gaatcttatc agtttttaga gcccatattt
      361 taaactggta atgatggggt ttatctcctt tgggaggaca tgctccacca aatcccgcat
      421 agccaaaatc atttcggcct tggacagcac cagtcggaag ttttgtacca tcacgtcttc
      481 ctgcatcagc gggcaaatat gttaccgttg ctggaatatt agcaacagtc cagtgccacc
      541 atccgctgcc tgtaggcgca tctggatcat acacagttac ggcaaagctt ttggtgcctt
      601 ctggagcacc agaccaggtt aatgagggcg atgtattacc cccctcacac ccaactccag
      661 aaaatacatg agacgttgta agttgctctc ctgtttttat ttcattacta gtgacctgaa
      721 atgctgcggc ctgcgcagaa aatgttatga atgccaatac acttgaaacg ataagttttt
      781 tcataaaaac ctctttgttg tgacctatcg ttattttatt tgatgtgcat tgatctcatt
      841 atgcaaaaag gcgcaatgat cgcgcaaatg caatcataat tataaagttg ccccgaacat
      901 tagccataat atgcacaaat cagattcctg ttatctcaag gatgattatt ttttaatgcg
      961 gtgcgcttcg tgacaacaag ttaatgacta acacaccggc acaaatcaac atcatgccta
     1021 tgatggctgg caggtccagt cgttggccaa aaaatcccca ggaaagcaaa ctaatcagaa
     1081 cgataccgac tcctgaccag atagcataag caatccctgt aggtatataa gccagtgtct
     1141 gagctaataa ccaaaatgat gcacaatagc aaataattgt gccaacagag ggccataacc
     1201 gtgtaaaccc ctctgaaaac ttcattaagg ttgtaccaat gacctctgca agtattgcgc
     1261 caccaagata aatataaggg ttcatagcat attctttcct gttcatactg gagagaattg
     1321 tactacagtt tgaacacaac tcaccgtttt catcctggtg tatgtgtatc cagggggctc
     1381 tggcgtggca agagataaag gcaggaacaa taatgatcag aaggaacccc aggctaacaa
     1441 aagagttcac tcttcctatg agcaaagcga agacaaaaca tgttatcgcg tttacggatc
     1501 gtggatggca gaaaactttc aggatcaggt atccatttta aaccagaaat tgagtgagtt
     1561 tgccccatcc atgccccacg cggtgagatc ggatgtaatt aacaacaggt tacaaaacct
     1621 acacctgcat ggacatcact tcctgataag ccgccaccag cttattacgc acctgaatcc
     1681 ccatttgcat agaaactgag gctttttgca tatcggtcat cacatcgttt aacgccacgc
     1741 cgggttcacc gagagtgaat ttttctgcct gcgtgcgggc ggctgtttgt gtatcgctta
     1801 tgcgatcgag cgcggcgtgc agctgcccgg caaaactaat ggtcggttgt ggcagtgatt
     1861 cctgcgcacg cgcactcatc gccgtagcct gtaactggct gataacccct tcaatcccct
     1921 gtatcgctga cattttcatc tcccggataa tttctggtag caaagcttac cagtaagtca
     1981 ataagacaaa ggcgctaaat agcaacaaaa aaaacgggtt tattggcgga tagaaaaaaa
     2041 cgaaagcaca aataatggca gcgtcaattt ttcgagtttg ctgacccggg agtgagtctt
     2101 gttccacttt gccaataacg ccgtccataa tcagccacga ggtgcgcgat gaatgcgact
     2161 gcagcccaga caaaatctct tgagtggctt aatcgcctgc gtgcgaatcc gaaaattcca
     2221 ttgattgttg ccggttccgc ggcagtggcg gtcatggtcg cactgatcct gtgggcgaaa
     2281 gcccccgact accgcacatt attcagcaat ctttccgatc aggatggtgg cgcaattgtc
     2341 agccaactga cgcaaatgaa tattccttac tgcttcagcg aagccagcgg cgctattgaa
     2401 gttccggcag ataaagttca cgaactgcgt ctgcgcctgg cacaacaagg tttgcccaaa
     2461 ggcggcgcgg tcggtttcga actgcttgat caggaaaagt ttggtatcag ccagttcagc
     2521 gaacaggtga attatcagcg ggcgctggaa ggcgagcttt ctcgtaccat cgaaactatc
     2581 ggcccggtaa aaggggcgcg cgtacatctg gcaatgccga aaccgtcttt attcgtccgt
     2641 gaacaaaaat ccccttctgc atcggtgacg gtaaatctgt tacccggccg cgcactcgat
     2701 gaagggcaaa ttagcgccat tgtgcacctg gtttccagcg ccgttgctgg tctgccgccg
     2761 ggaaacgtca cgctggtgga tcagggcgga catctgttaa cccagtccaa taccagcggg
     2821 cgcgatctta atgacgctca gttgaaatat gccagcgatg tcgaaggccg tattcagcgg
     2881 cgtattgaag caatcctgtc gcctattgtt ggtaacggta atattcacgc ccaggtcacg
     2941 gcgcaactgg acttcgccag taaagaacaa acggaagaac agtatcgccc taacggtgat
     3001 gaatctcatg cggcgcttcg ttcacgccag cttaatgaga gcgagcaaag cggttccggt
     3061 tatccgggcg gcgtaccggg ggcgttgtcg aatcaaccgg cacctgcgaa taacgcgcca
     3121 atcagcacgc ctccggcaaa tcaaaataac cgccagcagc aggcgagcac caccagcaat
     3181 agcgggccgc gtagcacaca gcggaatgaa accagtaact acgaagtcga tcgcaccatt
     3241 cgtcatacca aaatgaacgt gggcgatgtg caacgtctgt cagtcgcggt agtggtgaat
     3301 tacaaaacct tgccagacgg taaaccattg cctctcagca acgaacagat gaagcaaatt
     3361 gaagatctga cccgcgaggc gatgggcttt tctgaaaaac gcggtgactc gctcaatgtc
     3421 gttaactcgc cgttcaatag cagtgacgaa agcggcggag aactgccgtt ctggcaacag
     3481 caagcgttta tcgatcagtt gctggctgcc ggtcgctggt tgctggtact gctggtggcg
     3541 tggctgctgt ggcggaaagc ggtacgcccg cagttaacac gtcgcgccga ggcgatgaaa
     3601 gctgtacagc aacaggcgca ggcccgtgag gaagtggaag atgcggtgga agtccgcctg
     3661 agcaaagacg aacaactcca acaacgacgc gctaaccaac gtctgggggc agaagtcatg
     3721 agccagcgta tccgtgaaat gtctgataac gatccgcgcg tggtggcgct ggtcattcgc
     3781 cagtggataa ataacgatca tgagtaacct gacaggcacc gataaaagcg tcatcctgct
     3841 gatgaccatt ggcgaagacc gggcggcaga ggtgttcaag cacctctccc agcgcgaagt
     3901 gcaaaccctg agcgctgcaa tggcgaacgt cacgcagatc tccaacaagc agctaaccta
     3961 tgtgctggcg gagtttgagc aagaagctga acagtttgcc gcactgaata tcaacgccaa
     4021 cgattatctg cgttcggtat tggtcaaagc tctgggtgag gaacgtgccg ccagcctgct
     4081 ggaagatatt ctcgaaactc gcgataccgc cagcggtatt gaaacgctca actttatgga
     4141 gccgcagagt gccgccgatc tgattcgcga tgagcatccg caaattatcg ccaccattct
     4201 ggtgcatctg aagcgcgcgc aggccgccga tattctggcg ttgttcgatg aacgtctgcg
     4261 ccacgacgtg atgttgcgta tcgccacctt tggcggcgtg cagccagccg cgctggcgga
     4321 actgaccgaa gtactgaatg gcttgctcga cggtcagaat ctcaagcgca gcaaaatggg
     4381 cggcgtgaga acggcagccg aaattatcaa cctgatgaaa actcagcagg aagaagccgt
     4441 tattaccgcc gtgcgcgaat tcgacggcga gctggcgcaa aaaattatcg acgagatgtt
     4501 cctgttcgaa aatctggtgg atgtcgacga tcgcagcatt cagcgtctgt tgcaggaagt
     4561 ggattccgaa tcgctgttga tcgcgctgaa aggggccgag cagccactgc gcgagaaatt
     4621 cctgcgcaat atgtcgcagc gtgccgccga tattctgcgc gacgatctcg ccaaccgtgg
     4681 tccggtgcgt ctgtcgcagg tggaaaacga acagaaagcg attctgctga ttgtgcgccg
     4741 ccttgccgaa actggcgaga tggtaattgg cagcggcgag gatacctatg tctgataatc
     4801 tgccgtggaa aacctggacg ccggacgatc tcgcgccacc acaggcagag tttgtgccca
     4861 tggtcgagcc ggaagaaacc atcattgaag aggccgaacc tagccttgag cagcaactgg
     4921 cgcaactgca aatgcaggcc catgagcaag gttatcaggc ggggattgcc gaaggtcgcc
     4981 agcaaggtca tgagcagggc tatcaggaag gactggccca ggggctggag caaggtctgg
     5041 cagaggcgaa gtctcaacaa gcgccaattc atgcccggat gcagcaactg gtcagcgaat
     5101 ttcaaactac ccttgatgcg cttgatagtg tgattgcttc gcgcctgatg cagatggcgc
     5161 tggaggcggc acgtcaggtg atcggtcaga cgccaacagt ggataactcg gcactgatca
     5221 aacagatcca acagttgttg cagcaagaac cgttattcag cggtaaacca cagctgcgcg
     5281 tgcacccgga tgatctgcaa cgtgtggatg atatgctcgg cgcaacctta agtttgcatg
     5341 gctggcgctt gcggggcgat cccacactcc atcctggcgg ctgtaaagtc tccgccgatg
     5401 aaggcgatct cgacgccagt gtcgccactc gctggcaaga actctgccgt ctggcagcac
     5461 caggagtggt gtaatgacca cgcgcctgac tcgctggcta accacgctgg ataactttga
     5521 agccaaaatg gcgcagttgc ctgcggtacg tcgctatggg cgattaaccc gcgctaccgg
     5581 gctggtgctg gaagccaccg gattacaatt gccgctcggc gcaacctgtg tcattgaacg
     5641 ccagaacggc agcgaaacgc acgaagtaga aagcgaagtc gttggcttta acggtcaacg
     5701 gctgttttta atgccgctgg aggaagtgga aggtgtcctg cccggcgcgc gggtttatgc
     5761 caaaaacatt tcggctgaag gactgcaaag cggcaagcag ttgccgctcg gtccggcgtt
     5821 attaggtcgc gttctggacg gcagcggtaa gccgctcgat ggcctgccct cccccgatac
     5881 gacggaaacc ggtgcgctga ttaccccgcc atttaacccg ttgcaacgta caccgattga
     5941 acatgtgctg gacaccggcg tgcgcccaat caatgccctg cttaccgttg ggcgtgggca
     6001 gcgtatgggg ctgtttgccg ggtccggcgt tggtaaaagt gtgctgctgg ggatgatggc
     6061 acgttacacc cgcgccgatg tcattgtcgt gggtttgatt ggtgaacgtg ggcgcgaagt
     6121 aaaagatttt attgagaata tcctcggtgc tgaagggcgt gcacgctccg tggtgattgc
     6181 cgctccggcg gatgtctctc cgcttctgcg aatgcaaggt gccgcctatg ccacgcgcat
     6241 tgccgaagat tttcgcgatc gtggtcagca tgtgttgctg attatggact ccctcacccg
     6301 ctacgcgatg gcccagcgtg aaattgcgct ggcgattggc gaaccacccg ccaccaaagg
     6361 ttatccaccg tcggtgtttg ccaaattacc ggcactggtc gagcgtgccg gaaatggcat
     6421 tagcggcggc ggctcgatta ccgcgtttta taccgtgctt actgaaggcg atgaccagca
     6481 ggaccccatt gcagactccg cacgggcgat cctcgacggt cacattgtgc tgtctcgccg
     6541 actggcggaa gccgggcact atccggctat cgatattgaa gcgtcgatca gccgcgcaat
     6601 gacggcgttg atcagtgagc aacattacgc gcgagtgcgc accttcaaac agctgttgtc
     6661 gagttttcag cgtaaccgcg atctggttag cgtcggcgcg tatgccaaag gcagcgatcc
     6721 gatgctcgat aaagccatcg ccctgtggcc gcagctggag ggctatttac aacaaggcat
     6781 ttttgaacgt gcggactggg aagcgtctct ccaggggctg gagcgtattt tcccgacagt
     6841 gtcataaccc aggagataac ggtagatggc agaacatggt gcgctggcga ccctgaaaga
     6901 tctggcagaa aaagaggtag aggatgccgc gcgcctgctg ggtgaaatgc gtcgcgggtg
     6961 tcagcaggcg gaagaacagc tcaaaatgct gatcgattat cagaatgaat atcgcaataa
     7021 cctcaacagc gatatgagtg ccgggatgac cagcaaccgc tggatcaact atcagcagtt
     7081 tatccagacg ctggaaaaag ccattactca gcatcgccag caacttaatc agtggacgca
     7141 gaaagttgac attgccctga acagctggcg agaaaagaaa caacgtttgc aggcctggca
     7201 gacactgcag gaacggcaat ccacggcggc actgcttgca gaaaaccgcc tcgatcagaa
     7261 aaagatggat gagttcgccc agcgcgccgc catgaggaaa cctgaatgat tcgcttagcg
     7321 cccttgatta ccgccgacgt tgacaccacc acattgcctg gcggcaaagc cagcgatgct
     7381 gcacaagatt ttctcgcgtt gttgagcgaa gcattagcaa gcgagacaac taccgacaaa
     7441 gcggcccccc aggtgctggt ggcaacagat aagcccacga caaaaggcga gctgctgatc
     7501 agcgatattg tttccgacgc gcaacaagcg gatttactga tccctgtgga tgaaacactg
     7561 cctgtcatca acgtcgaaca atccacatcg acaccgttaa ccaccgctca tacgatgacg
     7621 ttggctgcgg tggctgacaa aaatacgaca aaagacgaaa aagcggatga tctgaatgaa
     7681 gacctcaccg caagcctgag cgcccttttt gcgatgttgc cgggttttga caatacgccc
     7741 aaagtgactg atgcgccgtc aaccgtgtta ccggcagaga aaccaacgct cttcacaaaa
     7801 ctgacttctg cgcaactcac aacagcacag cctgatgatg cccccggcac gccagctcag
     7861 ccattaacac cgctggtagc agaagcccag agtaaagcgg aagtcatcag cacaccttca
     7921 ccggtgaccg ctgccgccag cccgctaatc actccacacc agacacagcc actgctcacc
     7981 gtcgccgcgc ctgtcttgag tgcaccgctg ggttctcacg aatggcaaca atcattaagc
     8041 cagcatattt cgctgttcac ccgccaggga caacaaagtg cagagttgcg tctgcacccg
     8101 caggatttag gtgaagtgca aatctccctc aaagtggatg ataaccaggc gcaaatccag
     8161 atggtttcgc cgcatcagca cgtacgcgcc gccctggaag cagcgctgcc ggtactgcgt
     8221 acgcagctgg ccgagagtgg cattcagtta gggcaaagca acatcagtgg cgaaagcttt
     8281 agtggtcagc agcaggccgc ttcccaacaa cagcaaagcc aacgcacagt aaaccatgaa
     8341 cctctggcgg gggaagaaga cgatacgctt ccggttcccg tctctttaca agggcgcgta
     8401 acaggcaaca gcggcgttga tattttcgcc taacgtcaga ggtagcaccg taatccgcgt
     8461 cttttccccg ctttgttgcg ctcaagacgc aggataatta gccgataagc agtagcgaca
     8521 caggaagacc gcaacacatg actgattacg cgataagcaa gaaaagcaag cgatcgcttt
     8581 ggctcccgat tctggtattc attaccctcg cggcctgtgc cagcgcaggt tacagctact
     8641 ggcattcgca tcaggttgcc gctgaagaca aagcgcagca tcgcgtcgtg ccctcaccgg
     8701 tcttctatgc gctggatacc tttacggtca atttgggcga tgcggatcgc gtgctttata
     8761 tcggtataac cctgcgactc aaagatgaag ctacccgctc gcggctgagt gagtatttgc
     8821 cggaagtccg tagccgtttg ctgttactgt tttcgcgtca ggatgctgcc gtactggcga
     8881 cagaagaagg caagaaaaac ctgattgccg agattaaaac cacgctttcc accccgcttg
     8941 ttgccgggca accgaaacag gatgtcaccg acgtgatgta taccgctttt attctgcgat
     9001 aacgacatgg gcgatagtat tctttctcaa gctgaaattg atgcgctgtt gaatggtgac
     9061 agcgaagtca aagacgaacc gacagccagt gttagcggcg aaagtgacat tcgtccgtac
     9121 gatccgaata cccaacgacg ggttgtgcgc gaacgtttgc aggcgctgga aatcattaat
     9181 gagcgctttg cccgccattt tcgtatgggg ctgttcaacc tgctgcgtcg tagcccggat
     9241 ataaccgtcg gggccatccg cattcagccg taccatgaat ttgcccgcaa cctgccggtg
     9301 ccgaccaacc tgaaccttat ccatctgaaa ccgctgcgcg gcacagggct ggtggtgttt
     9361 tcaccgagtc tggtgtttat cgccgtggat aacctgtttg gcggcaatgg acgcttcccg
     9421 accaaagtgg aaggtcgcga gtttacccat accgaacagc gcgtcatcaa ccgcatgtta
     9481 aagctggcgc ttgaaggcta tagcgacgcc tggaaggcga ttaatccgct ggaagttgag
     9541 tacgtgcgtt cggaaatgca ggtgaaattt accaatatca ccacctcgcc gaacgacatt
     9601 gtggttaaca cgccgttcca tgtggagatt ggcaacctga ccggcgaatt taatatctgt
     9661 ctgccattca gcatgatcga gccgctacgg gaattgttgg ttaacccgcc gctggaaaac
     9721 tcgcgtaatg aagatcagaa ctggcgcgat aacctggtgc gccaggtgca gcattcacag
     9781 ctggagctgg tcgccaactt tgccgatatc tcgctacgcc tgtcgcagat tttaaaactg
     9841 aaacccggcg acgtcctgcc gatagaaaaa cccgatggca tcatcgccca tgttgacggc
     9901 gtcccggtgc tgaccagtca gtacggcact ctcaacggtc agtatgcgtt acggatagaa
     9961 catttgatta acccgatttt aaattctctg aacgaggaac agcccaaatg agtgacatga
    10021 ataatccggc cgatgacaac aacggcgcaa tggacgatct gtgggctgaa gcgttgagcg
    10081 aacaaaaatc aaccagcagc aaaagcgctg ccgacgcggt gttccagcaa tttggcggtg
    10141 gtgatgtcag cggaacgttg caggatatcg acctgataat ggatattccg gtcaagctga
    10201 ccgtcgagct gggccgtacg cggatgacca tcaaagagct gttgcgtctg acgcaagggt
    10261 ccgtcgtggc gctggacggt ctggcgggcg aaccactgga tattctgatc aacggttatt
    10321 taatcgccca gggcgaagtg gtggtcgttg ccgataaata tggcgtgcgg atcaccgata
    10381 tcattactcc gtctgagcga atgcgtcgcc tgagccgtta gtgatgaata accacgctac
    10441 tgtgcaatct tccacgccgg tttctgctgc gccactgctg caggtcagcg gcgcactcat
    10501 cgccattatt gccctgatcc tcgctgctgc ctggctggta aaacggttgg gatttgcccc
    10561 gaaacgcact ggcgttaacg gtctgaaaat tagcgccagt gcttcgctgg gcgcgcgtga
    10621 acgggttgtg gtggtcgatg tggaagatgc acggctggtg ctcggcgtta ccgcagggca
    10681 aatcaatctg ctgcataaac ttcccccttc agcaccaaca gaagagatac cgcagaccga
    10741 ttttcagtcg gtcatgaaaa atttgcttaa gcgtagtggg agatcctgat gcgtcgtttg
    10801 ttgtctgtcg cacctgtcct tctctggctg gttacgcccc tcgccttcgc gcaactgccg
    10861 ggtatcacca gccagccgct gcctggcggt ggacaaagct ggtcgctccc ggtgcagacg
    10921 ctggtgttca tcacctcgtt gacgtttatt ccggcaattt tactgatgat gaccagtttc
    10981 acccgcatca tcattgtttt tggtttattg cgtaacgcgc tgggaacgcc ctccgcgcca
    11041 cctaaccagg tattgctggg gctggcacta tttttgacct tttttattat gtcatcggtg
    11101 atcgacaaaa tttatgtcga tgcgtaccag ccattcagcg aagagaaaat atcaatgcag
    11161 gaggcgctgg aaaaaggggc gcagccgcta cgtgagttta tgctgcgtca gacccgtgag
    11221 gcagatttag ggttgtttgc cagactagcg aataccggcc cgttgcaggg acctgaagcc
    11281 gtgccgatgc gcattttgct cccggcctac gtgaccagcg aattgaaaac cgcatttcag
    11341 ataggcttca cgattttcat tccttttttg attatcgacc tggtgattgc cagcgtgttg
    11401 atggcattgg ggatgatgat ggtccccccc gccaccattg ctctgccctt taaactgatg
    11461 ctgtttgtac tggtggatgg ctggcaattg ctggtcggtt cgctggcgca gagcttttac
    11521 agctagagag gcaaaatgac acctgaatcg gtcatgatga tggggactga agcgatgaaa
    11581 gtcgcgctgg cactggctgc cccgctgttg ctggtggcgc tggtcacggg ccttatcatc
    11641 agtattttgc aggccgccac gcagatcaac gaaatgacgc tgtcgtttat tccaaaaatc
    11701 atcgccgtat ttattgccat tattattgcc ggaccgtgga tgctcaatct gttgctggat
    11761 tacgtccgca ccttgttcac taacctgccg tatatcatcg ggtaggccgt acaatgatgc
    11821 aggtgacaag cgatcaatgg ctttcctggt taagcctgta cttctggccg ttactgcgcg
    11881 tgctggcgct catttctacc gcaccaattc tgagcgaacg cagcgtgccg aaacgggtaa
    11941 aactggggct ggcaatgatg atcacgttcg tcattgcccc atcattacct gccaacgatg
    12001 ttcctgtttt ttcgttcttt gctctgtggc tggccgtgca gcagatcctg atcggcattg
    12061 cgcttggttt taccatgcaa tttgcctttg ccgctgtgcg aaccgctggc gaaattatcg
    12121 gtctgcaaat ggggctgtca tttgcgacgt ttgtcgatcc ggccagccat ctcaatatgc
    12181 ccgttttagc gcgtatcatg gatatgctgg cgttactgct gttcctgaca tttaacggtc
    12241 atttatggtt gatttcactg ctggtcgata cctttcacac cctgccaatt ggtggcgaac
    12301 cgttgaacag caatgcgttt ctggcactca ccaaagcggg gagtttgatt ttccttaacg
    12361 ggctgatgct ggcgttaccg ctcattactc tgctgctgac cctgaatctg gcattaggtt
    12421 tactgaatcg tatggccccg caattatcca tttttgttat tggatttcca ttaactctga
    12481 ctgtcggtat ctctttaatg gcggcattaa tgccgttaat tgcacctttt tgcgaacatt
    12541 tattcagtga gatgtttaat ttgctggctg atattattag tgaattgcca ttaatataat
    12601 tccgtaacgt ttatcatgtt atcctaagga ttatccgaaa aataatacct acgaacatct
    12661 tccaggatac tcctgcggcg aaatatttgt tttaagctca ctcacatatc gcaacattta
    12721 ctttaagaca attccaggca aattatacaa cactttacgg gatagtaagt ccgcctgaaa
    12781 aatcgcgaga gtggcgcatt aggtgaccca tgttgttccg tttagtcatg atgaaatatt
    12841 caggtaaggg gaattatcgt tacgcattga gtgagggtat gccatgtcaa cgattattat
    12901 ggatttatgt agttacaccc gactaggttt aaccgggtat ctgttgagta gaggggttaa
    12961 aaaaagagaa atcaacgaca ttgaaaccgt tgatgacctt gccatagctt gtgattcaca
    13021 gcgcccttca gtggtgttta ttaatgagga ctgtttcatc catgatgctt ctaacagtca
    13081 gcacatcaag cacatcatta atcaacatcc caatacgtta tttatcgttt ttatggcaat
    13141 tgccaatgtt cattttgatg aatatctatt ggtcagaaaa aatttattga taagttctaa
    13201 atcgattaaa ccggaatctc tcgacgatat ccttggcgat attctgaaaa aagagacaac
    13261 gataacctct tttttaaata tgccgacgtt atcattgagc cgaaccgaat cgagtatgtt
    13321 gcgaatgtgg atggcaggtc agggaaccat tcaaatctct gaccaaatga atatcaaagc
    13381 caagaccgtt tcatcgcata aaggtaatat taaacgtaag atcaaaacac ataataaaca
    13441 ggttatctac catgtcgtcc gactgacgga taatgtgact aatggtattt ttgtcaacat
    13501 gcgctaacac attctgactg gtggtttccc accagtcagg ctgaataaga ttactctgct
    13561 ttctccacaa agataccgtc ctgatgccct gcttcattaa agaaccagat gccgagcggg
    13621 tagtcttcca gcgaaaccag gtacattgtg ccttcactaa actcctcaac tgccagtacc
    13681 acgccaggac gacgcggacc gccatccgtt ttgactgtta cccgatcatt caccttcatc
    13741 gttttcctcc tgtggctttg tgccagtgta gaacaatttc gtttttctgg cagctccagg
    13801 cgcgcgcgag tgctgatttt ctcgacggtc tatacttaag agatgccagg cggacttaac
    13861 gactggcggc aacaacagag taacggttgc gaggaaagat gatgaaaacc gcaaaagagt
    13921 acagcgatac cgcaaaacgt gaggtcagcg tcgatgtcga tgccctgctg gcggcgatca
    13981 atgaaattag cgaaagcgaa gttcatcgca gccagaacga ttctgaacac gttagcgtcg
    14041 atggacgtga atatcataca tggcgtgaat tggcggatgc cttcgaactg gatattcatg
    14101 acttcagcgt ctctgaagtg aatcgttgaa tgcacaataa aaaaatcccg accctgaggg
    14161 ggtcgggatg aaacttgctt aagcaagaag cacttaaaaa attcgttaca ccaggaaatc
    14221 tgatgtgttc atcaccttat ccgcaatttt tttcgctgac aagaaaatat tcgcgatatg
    14281 aataattaac gctacttatg aatagaaatg tgacgtgcgt caccccttgg tggctctggc
    14341 gtcaacggga tcgctcccga aaaaatcatc tttgattatt tgtgctgttt tgagaatcat
    14401 cctggtaagg ggagaactgc acgaccatgt tttcaattca acaaccacta ctggttttta
    14461 gcgatcttga tggcaccctg ctggacagtc atagttatga ctggcaaccg actgccctct
    14521 ggctcagccg tttacgcgaa gcgaatgttc ccgtcattct ctgtagcagt aaaacttcag
    14581 cggaaatgct gtacttgcaa aaaacgttgg ggctacaagg gttaccactg attgccgaaa
    14641 atggcgcagt gatccagctt gctgaacaat ggcaggatat agacggtttt ccacgcatca
    14701 tctcaggtat tagccatggc gaaatcagcc aggttttaaa tacgctacgc gagaaagaac
    14761 attttaaatt cacgactttc gatgatgtcg acgatgcaac catcgccgaa tggacgggat
    14821 taagccgtag ccaggcggcg ctgacgcagc tgcatgaggc gtcggtaacg ctaatctggc
    14881 gcgacagtga cgagcgtatg gcacaattta ccgctcgtct gaacgaactg ggcttacagt
    14941 ttatgcaggg tgcgcgcttc tggcacgtcc tggatgcctc tgccggaaaa gatcaggccg
    15001 ccaactggat tatcgcgacc tatcaacaat tatcaggcaa acgcccaacc acacttggcc
    15061 tgggcgatgg gccaaacgat gcgcccttac tggaggtaat ggattacgcg gtgattgtga
    15121 aagggctaaa ccgtgaaggg gtgcatctgc atgatgagga tccgacccgc gtctggcgaa
    15181 cgcagcgtga aggaccggaa ggctggcgtg aagggctgga ccattttttc tccgctcgtt
    15241 aagcgttatc gctcgcgcat acccgattac gcccggcctg tttagcgaga taaagccgac
    15301 ggtcagccag tgactggagt tgttcaaaat cataatcacc ggtttcctcg ctgctactta
    15361 cccccaacga ggcactgatg cgtatcgtcg tactcttagc aatcaacatc tctttttcat
    15421 ttaacttcag gcgaatacgt tctgcgactt ccgcagcctc cgtcagactc gcgcctggca
    15481 gaatcacaca aaactcctca ccaccgaccc gcccggcaac gtcctgcgca cgcaaggaac
    15541 tgctaattaa tccggcagca tgagaaagaa cacggtcgcc cgcctgatga ccaaagcggt
    15601 cattaatcgc tttaaaatgg tcaaggtcga cctgaatgac agaaaaagga tgttggtgcg
    15661 tctgacacaa tttcgcgagc ggacgggctt tttcaaacag tgcgccacga ttatataaac
    15721 gggttaaggt gtcgtgccac gcctgccact gcaacgagct ttgcagaaca tacatgttgc
    15781 taaccatccg gcgaatcaca taccaggaga tgagtaacat ggtggtaaag agcgcccaca
    15841 gcagggttaa tgcaatgctg atactgccga aatcgccgcg cacgccttcg cttagcgtat
    15901 ggacgcgcac cagcacaccg tcgaaatggt ccagacgttc ccagctaaca tagcgactgt
    15961 ccatacgaat gccgccccgc gtgtcatgtt ccatcgcctg cgccagcaag gccagttcac
    16021 gaggatcaaa aatattccct gttggatggt caggattgga agaggtcaaa aatctcagct
    16081 tactgtcata gagctgatac tcaccatcga ggtttttatc gatggcgttt cttaaaaatt
    16141 gctgcatggt acgcacggga atactcatcc ccagcacgcc ataccagtag ttattactgt
    16201 ctaccggaac actgacggta acctgcggtt cagtattgct ggcgtgttcc ggttgcgaag
    16261 taaaccagcg taccgcgcgg tgacgatttt ctcgttgcga atggccgata aaccagggtt
    16321 gggtgacata gccgtaataa cgcgttggta cattgcgcgt aaacaaggtc ggctgcgtcg
    16381 aaacgtaaaa tccggcacgc gagacataca tcgcctgttc aaccatcgac gaggtgttgt
    16441 gcgccagtcg cagcaagtaa ccaacttcca gtgcagcggt aatttcattg tcgaggcttt
    16501 cattttcgcg agacaggaga ttcccctcgc tgactaatgc atccgatacg ccattgactg
    16561 acagggtgcg tcgtcggttg agttcaattt gccaggcgtg ctcgtcgcga tgctgttcga
    16621 actcggttac agcattacgc agtgaagtga aatccagtgg cgctacgaga gcttcgcgca
    16681 tgccattacg caaaaagatc agtttgtcga cgttatactg caaatgctta tcgagcgcgt
    16741 tggctacgtt ttccagatga ttacgctggc tggagatata ggcatcttcc agcactacca
    16801 cttcacgcca ggtcagcaag gtggaaaaaa gcaataccac gataaagcag agattaacga
    16861 catgaccagg ccccaggcgg cgtgcgagtt ttttcaacca gctctggttt tccatttttg
    16921 tctcgtgctg caccctgacg actcccaacc tgctttttta tgattctggc ataacctggc
    16981 gatagcgtac cgcaaaacag cgataatttt ataatggtgg catgataaga cgcgactatc
    17041 attcattgca ggcattggca caccaggccg gaagtgatcc ggcctggtgt taatgatacc
    17101 ctttcaaact tcttccgcag aacgactccc ctcccccgga acaagctcgg tttcatgaaa
    17161 agcttcccgc ttgaccccgt aaccgtcata ccaacgacac tcaaccatac cgctggagta
    17221 tccagtgaca atcatccgcg ggccgccctc tttaaccgta acttcctcac taaccataaa
    17281 gctcatactc gcctcctttt ttcgagtgaa acttgttcac cttagttgaa gatggcgaat
    17341 tttgcataat agcctgtacg agtattaatg tgcctgaccg cgcattttcg ctacggcttt
    17401 cactcccagc accacgatgc cgccgatgat aaatccaaga atcagattta aaacagtcgg
    17461 taatatcatc gccaccactg cactttgctg cccggcgaaa tgttcaatgg cgtgatgcag
    17521 cggcgcaata ccatgtacca caataccgcc gccgacgagg aacatcgcca gcgtgccgac
    17581 aatcgataac gccttcatca accagggcgc aatgatcaat aaccctttac ctaatgcctg
    17641 catcagcgcg ctggattttt ccgccagcca ataccccagg tcatcaatct taacgataac
    17701 ccctaccaga ccgtagacgc ccacggtcac taccagcgcg atgcctgaaa gcaccagcac
    17761 ctgattaagc aacggcgctt cggccacaat ccccagcgtg atggcgacga tttcggcaga
    17821 caatataaaa tcggtacgaa tcgccccttt tattttgtcc ttttcaaact tcagcggatc
    17881 ctgcgccgcc agcttctcca gacgctgctg gctctgcgcc ggatcttctt tatgtttacg
    17941 cgcctccagc atatgcagca ctttctctac tccttcaaag cagagaaacg cgccaccaat
    18001 catcaacaga ggcgtaatcg cccacgggat aaacgcactg atgatcagcg ccagcggcac
    18061 cagaatcact ttattaatca gcgatccttt cgccacgccc cagaccacgg gaagttcccg
    18121 gttggcccgc acgcctgaaa cttgttgcgc attgagcgat aagtcatccc ctaatacacc
    18181 ggcggttttc ttcgccgcca gtttgcccat cacggagata tcgtccagta gtgtggcgat
    18241 atcatcgagc aacgtcagta aactactccc ggccaaaagc attccctctc atttttattg
    18301 ttgaagggtg taagtatgaa gcaaaaacgg cagctctgat acttgcttca caggtcaaca
    18361 ttttttttac aatcaagtgc aattatatct ctcgccagca gaatgatttt cacgtttact
    18421 atgaaccacc tttttatttc caccgtgagg gattatgcgt ttccgccagt tgttaccgct
    18481 ttttggcgcg ctgtttgcgt tgtatatcat ttggggctca acctattttg tcattcggat
    18541 tggcgtggaa agctggcctc cgttaatgat ggcaggcgtt cgattcctgg cagccggtat
    18601 tttattgatg gcatttttgc tactgcgcgg acacaagctc cccccgctac gtccgctgct
    18661 caatgccgcg ctgattggcc tgttattgct ggctgtcggt aatggcatgg tgacggttgc
    18721 cgagcatcaa aatgttcctt ccggcatcgc cgccgttgtg gttgcaaccg tgcccctctt
    18781 taccctggtg cttcagccgc ctgtttggca ttaaaacgcg caaactggaa tgggtgggta
    18841 tcgccattgg gcttgccgga atcatcatgc tcaatagcgg tggaaattta agcggcaatc
    18901 cgtggggcgc gattctgatt ttaatcggct cgattagctg ggcgtttggc tcggtttatg
    18961 gctcgcgcat taccttacct gtagggatga tggcgggtgc gattgagatg ctggcggcag
    19021 gcgtggtgtt aatgatcgcg tcgatgattg caggtgaaaa actgacggcg ctcccttccc
    19081 tttcaggctt ccttgcggtc ggctatctgg cgctgtttgg ttcgattatc gccatcaacg
    19141 cttatatgta tttaatccgt aatgtcagtc cggctctcgc caccagctac gcctacgtta
    19201 acccggtggt cgcggtcttg ctgggtacgg gactgggcgg agagacgctg tcgaagattg
    19261 aatggctggc gctcggcgta attgtcttcg cggtggtact ggtcacgttg ggaaaatatc
    19321 tcttcccggc aaaacccgta gttgcgccag ttattcagga cgcatcaagc gagtaaatga
    19381 atcccctgcg tgtcgatctg cgcgctggca ccttcgccgc agatccactc ttccagacgc
    19441 tcggtaagcg cttcatccgt cagcttctcg cgcccacgta atgcacactc ccagacaatc
    19501 agtacgcgcc agccgagttc ctgcaaacga ctgatatcgc ggcgatcgcg ttcaacattt
    19561 ttacctatct tctccagcca gaactcggtt cgagtcgcag gcactttaaa cagatagcag
    19621 tgatgatgat gccagaagca gccatgggta aatatcacgc agcgatattc atcaacgaca
    19681 aaatccgggc ttccgggcag actggcgtcc tgaacgcgaa acgccaggcc ctgcccggtt
    19741 aacagactgg cgaggcgctt ctcaatcgcc gtatcacgcg tggcaatcgc gcgcatattt
    19801 ttgctgcgag tggccttatc gtgaacgtcg gccatgttgt gcctcttgct gacgcaacgc
    19861 caccgcctgt ttgatttttg gctcaagcag ttttgccacc gcggcaaaga ccggcacgac
    19921 caccgagtta ccgaactggc gataggcctg agtgtccgaa accggaatgc ggaatttcgc
    19981 ttctcccggc gcttcaaaac ccattaagcg cgcgcattcc cgaggcgtta accgacgtgg
    20041 acgatgttgc tgattcagcg gatcgtcaaa atctttctca cccgtggcca tatcccagcc
    20101 gcgatcgatt aaaatttccg cgccatcttt gtaataacgc gcagacagcg tacgcgtgac
    20161 gctttgcgga ttgttcggat aaaccattcc ataaccgaag ccgttaccgc gcgcctgatg
    20221 ttttttcgca tatcgataga ggtacttcca cagcaccggc gtcaggatat atttcgcctc
    20281 gaccatcggg tccaacagct gcgccagcgt cactcgctgt gcagggaaac attcgctgat
    20341 atcacgcaga gtaaaatcgg ctttcagatt aagatcgcga cgaaaaccca ccagcacgat
    20401 gcgttcacgg tgctgcggca gaaaatgttt accatcgatg attttcggat cgtccggccc
    20461 gttatcttct gcatcagcca cgtcatagcc cagttcgtcc agcgtctgca tgatgatgcg
    20521 gaacgtttta ccctggtcgt gacttttcag gtttttgacg ttttcgagca caaacatcgc
    20581 cggacgacgc gcgtcgataa tgcgtaccac atcaaaaaac agcgtgccct gggtatcgca
    20641 ggcaaaaccg tgcgcccgcc cgagcgagtt ctttttcgat acgccagcca gcgaaaatgg
    20701 ctgacaaggg aaaccggcca gtaaaacatc gtgttcagga atgtgttgac gaatatgttc
    20761 cgccgccgcc tcatcactca cgccttcttt atggctgagg gtgatgtcgc ggatatcttc
    20821 attaaaatga tgcgtcgccg gatcgcaata atggttggct ttataagtgc gtaccgcatg
    20881 tttgttccat tcgctggtaa acacgcattg tccgccaatc gattcaaaac cgcgacggat
    20941 gccgccaatt ccggcaaata gatcgataaa gcgaaacgca taatgcggat gatgtgccga
    21001 tggtttgggt aacaacgttt gcagatgggc gaactctttc tcacttaaac ggtgccatgc
    21061 cgagtcattc gccagcgcac gttttaaaat tgccgcgctc cagtgattct cacccacacc
    21121 attaagctgc gccaccagcg ttttaacatc ataaatttgc agcagtttct ccagcattgc
    21181 ctgtgcggca ttcccggtgc tgtatgaatc ggttactgat atattttcct gcatagattt
    21241 aaccggccat ctgagatggc cgacaggtta acataattac agccatttag ggccacgcgc
    21301 ttatcccgcg ggtgaaaacg catctatcac cttgtgatcg acaccttcat tctcccccgc
    21361 cagttcggca ctgagcttcg ccataaactc gactaaaaaa tgcgcgttgt gctgcgccag
    21421 ctgcttgcct cttgcggttt gcatggtttg cggtagcttc agcaacttag tctggaaatg
    21481 atccagcgcg tagcgtttat catcaagcgg gcgatgctgt gcgaacggat cttcaccatc
    21541 aaacagcgcc acgcccaatg cccctgaaac ggcaaacacg cgcgccaggc caatcgcccc
    21601 cagggcttcc agccgatccg catcctggac gattttagct tccgttgtta agggggcgat
    21661 ttgcgcgctg aaactgtgag cggcaatggc atgacaaacg gcctcgattt tctccgccgg
    21721 aaattgctcg aactcttcac ggagcaggcg acgcgtctct tctgccgcca ggattgaaga
    21781 acgctgcctt tgcggatgat ttttcgccag gctcacaata tcgtgaaaat aacaggcggt
    21841 taaaatcacc agcatatcga catcgtcatc tgccgccagc ttctgtgccg cgtcctggtg
    21901 ctgatgatgg tttttcaacc agttttcgaa ctgtgcctgc cagtgttgta agtccatacg
    21961 cgctccgttg ccaaagagtc gccaccttag cagtttttat tcatagcgtc tgctacgtgc
    22021 gggacgaggt cgggtgtacc aggcaatgct gcctaacaat gccccgacca gagccataaa
    22081 taaaaatccg accagtgccc ctaaccactt tcccgcggta gagcctaaat cgctttcaaa
    22141 tcccgacacc ggggcatcag ggatccggct catcacccag atgtaacgcg ccattgccca
    22201 gacgaaatag ccacaccatg tatagaaagc ccacaaagcc aatttgccgc cagggctgcg
    22261 agagagtctc tcttccatct ttgtcgaacc gtattccatt ttgagataac ccggaaaatt
    22321 cagacttagt actattatgt gataaaagtc acatttttcc acattggatg agccagtcaa
    22381 gactcaatca ttttttgaac aaatagataa attttgcgca acggcaataa ttactggaaa
    22441 aacagcaata aagatttatt tatatccttg gtaatagttt tattaattca cccaaacaat
    22501 atattagcac tgaatacaat tatacctttt ttgatttctg tgtcatgata accccgttat
    22561 tagcctttta tcgtcttgtt tatatttttt gggccggcat gatgccggct ttttttatac
    22621 cttcattaat gtgcgcgtga tcacgcctgc cgtttggcgc aacaatcatt gatactccct
    22681 atctt
//
LOCUS       DAZPDU010000057        22392 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZPDU010000057 DAZPDU010000000
VERSION     DAZPDU010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22392
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.057"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            231..1664
                     /gene="cscA"
                     /locus_tag="ACHURS_001997"
     CDS             231..1664
                     /gene="cscA"
                     /locus_tag="ACHURS_001997"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFR1237739.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDCAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2667)
                     /locus_tag="ACHURS_001998"
     CDS             complement(1672..2667)
                     /locus_tag="ACHURS_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR1237740.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="ACHURS_001999"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="ACHURS_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="ACHURS_002000"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="ACHURS_002000"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFR1237741.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNTTHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="ACHURS_002001"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="ACHURS_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFR1237742.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="ACHURS_002002"
     CDS             complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="ACHURS_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFR1237743.1"
                     /translation="MEQINSNKKQSSRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIRSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7687..8301
                     /gene="evgA"
                     /locus_tag="ACHURS_002003"
     CDS             7687..8301
                     /gene="evgA"
                     /locus_tag="ACHURS_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFR1237744.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGILVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8306..11899
                     /gene="evgS"
                     /locus_tag="ACHURS_002004"
     CDS             8306..11899
                     /gene="evgS"
                     /locus_tag="ACHURS_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFR1237745.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVILDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRTLNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYSTGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALICSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKNN"
     gene            complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="ACHURS_002005"
     CDS             complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="ACHURS_002005"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFR1237746.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="ACHURS_002006"
     CDS             complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="ACHURS_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFR1237747.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="ACHURS_002007"
     CDS             complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="ACHURS_002007"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFR1237748.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15936..17186)
                     /gene="frc"
                     /locus_tag="ACHURS_002008"
     CDS             complement(15936..17186)
                     /gene="frc"
                     /locus_tag="ACHURS_002008"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFR1237749.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDSAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17699..18322)
                     /locus_tag="ACHURS_002009"
     CDS             complement(17699..18322)
                     /locus_tag="ACHURS_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFR1237750.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18619..18894
                     /gene="ypdI"
                     /locus_tag="ACHURS_002010"
     CDS             18619..18894
                     /gene="ypdI"
                     /locus_tag="ACHURS_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HFR1237751.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18971..19213)
                     /locus_tag="ACHURS_002011"
     CDS             complement(18971..19213)
                     /locus_tag="ACHURS_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFR1237752.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19566..20486
                     /gene="lpxP"
                     /locus_tag="ACHURS_002012"
     CDS             19566..20486
                     /gene="lpxP"
                     /locus_tag="ACHURS_002012"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFR1237753.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNPKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20842..20913
                     /gene="ypdK"
                     /locus_tag="ACHURS_002013"
     CDS             20842..20913
                     /gene="ypdK"
                     /locus_tag="ACHURS_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFR1237754.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20978..22216)
                     /gene="alaC"
                     /locus_tag="ACHURS_002014"
     CDS             complement(20978..22216)
                     /gene="alaC"
                     /locus_tag="ACHURS_002014"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFR1237755.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDITEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
BASE COUNT         6319 a         5228 c         4871 g         5974 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgtcata
      121 gttaacgtta acatttgtga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaacaatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgccacctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagcg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gccaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat tgtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg gaccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cacaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tgttttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcggac aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacgg cgacatcctg
     1861 cgggatacgt acccccttcg ccaggagaac ctgataagcg acaaaggctg cacgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgggcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aggcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataacgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 tcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccag
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccgctgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctag
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctggcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa actgctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccagc tatgcaaaaa cggctggaaa aagaatatca
     3421 gcaaccgatc agcgggcaac tgttactgaa aaaagatagc catttgccca tttccggctc
     3481 cataaaagca cgcggcggga tttatgaagt cctggcacac gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gtcgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc aggctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc ggcactggcg ggtatggccg gacctcagcg
     4321 cgtatgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aactgcgtaa
     4381 taccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcagca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat acggaaaatc tcatttgcag aaatagaaag
     4681 tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccataata
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctgaaaa aactgcggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaacta aatgtcacta acaaccgcat
     5101 gtctattttg ttgccataac gtcctatcaa aggtgaaatt aatagtggca tgatgccgat
     5161 gggcgcataa gcaagtccgg cccatatcgc attatacccc atcgtttcct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg catgtgatac tcacaatacc
     5281 aatggtgaag ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ctcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcctggtag attcattttg accggtgaag tctcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc acaaatataa ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 cggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaaccc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataggctac acattaatga
     5821 agacagcgaa aaaaaggtta ctgaaagtaa aaataatctt aattcgccta ttctttgtgc
     5881 caacctgcca gtaacaggga tcgcaatggc atttgctaca ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaatgt cgctaatgac aatgcaatag tgacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagccggcat ggaggtcacg gttgaagcca gctcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccgggac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcgcc
     6661 aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaggatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tttctgctag actgtttttt atttgaatta
     7261 atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga
     7321 gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga
     7381 ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat
     7441 acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa
     7501 tcatcggtat aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg
     7561 tatacttgtc gaattatctt aaaggaagct cagattttct tatttttatt gagaaaatga
     7621 gatgacgcct tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa
     7681 taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat
     7741 ttattgatca aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg aagcgccgtt
     7801 cagcgggtgg aaacacttaa gcctgatatc gtcatcattg atgtcgatat ccccggagtt
     7861 aacggtatcc tggtgttaga aacgctgagg aagcgccaat atagcggaat tattattatc
     7921 gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatggt
     7981 ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat
     8041 ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag
     8101 caaaaactcg actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc
     8161 aaggataata atgacattgc ggaaaaaatg ttcatcagca acaaaactgt cagcacttat
     8221 aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgca
     8281 caacgtaaca aaatcggcta accacatgaa gtttttaccc tatatttttc ttctctgttg
     8341 tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag
     8401 tagtaacaac cgtgtcatac ttgatccagt acgtctgagc aacaaggaat tacgttggtt
     8461 agcgagcaaa aaaaatcttg tgattgcagt acataagtcc caaacggcta cgttgttgca
     8521 taccgattcg cagcaacgag ttcgtggtat taatgctgat tatttaaatc ttttaaaaag
     8581 aacgttaaat atcaaattaa cactccggga atacgccgat catcaaaaag caatggacgc
     8641 gctggaagaa ggtgaagtcg atatagtgtt atcacattta gtagcttcgc cgcctcttaa
     8701 tgatgacatt gctgcaacca aaccactgat aattaccttt ccggcgctgg taaccaccct
     8761 tcacgattca atgcgaccgc ttacctcatc aaaaccagta aatattgctc gagtagcaaa
     8821 ttatccccct gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta tctcttttac
     8881 aaatttatat caggcattag catccgtctc agccggacag aatgattact ttattggtag
     8941 taacatcatt accagcagta tgatttcccg ctatttcact cactccttaa atgtagtgaa
     9001 atattataac tcaccgcgtc aatataattt tttcttgacc agaaaagaat ctgtcgttct
     9061 taatgaagta ctcaatcgat ttgttgatgc tttgacaaat gaagttcgct atgaagtatc
     9121 acaaaattgg cttgatacag gaaacctggc ctttctgaac aaaccattag aactcactga
     9181 acatgaaaaa caatggatta agcagcatcc cgatttaaag gtgctggaaa atccttactc
     9241 ccctccctat tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg gggatattct
     9301 taatattatt actttgcaaa caggtttaaa tttttctccg atcaccgttt cacacaatat
     9361 ccatgctgga acacagctga atcccggcgg atgggatata ttacccggtg ctatttatag
     9421 tgaagataga gaaaataatg ttttatttgc tgaagccttc ataacaacgc cttacgtttt
     9481 tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa aaaggaatga aagttgccat
     9541 tccatattat tatgagcttc attcgcaatt aaaagagatg tatccggagg tagagtggat
     9601 aaaagtcgat aacgccagcg ctgcatttca caaggtcaag gaaggcgaac ttgatgctct
     9661 ggtcgcgaca cagttaaatt cacgttacat gatcgaccat tactatccta atgaacttta
     9721 tcattttctt attcccggcg ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga
     9781 accggaactt aaggatatta ttaataaagc actgaatgca atccccccaa gcgaagttct
     9841 gcgcctgacc gaaaaatgga ttaaaatgcc caatgtgacc attgacacat gggacctcta
     9901 tagcgagcaa ttttatattg ttacgacatt atccgtttta ttagttggca gtagcctttt
     9961 atggggattc tacctgttac gctcagttcg tcgtcgtaaa gtcattcagg gtgatttaga
    10021 aaaccaaata tcattccgaa aagcactctc ggattcctta ccgaatccaa cttatgttgt
    10081 aaactggcaa ggtaatgtca ttagtcataa tagtgctttt gaacattatt tcactgcgga
    10141 ttactacaaa aatgcaatgt taccattaga aaacagtgac tcccccttta aagatgtttt
    10201 ttctaatgcg catgaagtca cagcagaaac gaaagaaaat cgaacaatat acacacaggt
    10261 ttttgaaatt gataatggca tcgagaaaag atgcattaat cactggcata cattatgcaa
    10321 tctgccagcc agcgatcatg ctgtttatat ttgtggttgg caagatatta cagagacgcg
    10381 tgatttaatt catgcactcg aagtagagag aaataaagcg atcaatgcaa ctgtcgcaaa
    10441 aagtcagttt ctggcaacaa tgagtcacga aataagaaca ccaataagct ccattatggg
    10501 cttcctggaa cttctgtcgg gttctggtct tagcaaggag caacgggtgg aggcgatttc
    10561 acttgcctac tccaccggac aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga
    10621 caaaattgaa tcgggtaact atcaacttca accacaatgg gtcgatatcc ctactttagt
    10681 ccagaacact tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg cattaatttg
    10741 cagcagtaca tttcctgatc attacctggt taagatcgac cctcaggcgt ttaagcaggt
    10801 cttatcaaat ttactgagca atgctctcaa atttaccacc gagggggcag taaaaattac
    10861 gacctccctg gttcacattg atgacaacca cgctgttatc aaaatgacga ttatggattc
    10921 tggaagtgga ttatcgcagg aagaacaaca acaactcttt aaacgctaca gccaaacaag
    10981 tgcaggtcgc cagcaaacag gatctggctt aggcttaatg atctgcaaag aattaattaa
    11041 aaatatgcag ggcgatttgt cattagaaag tcatccaggc ataggaacaa catttacgat
    11101 cacaatcccg gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag cagaacaccc
    11161 catcacacta cctgaaaagt tgagcatatt aatcgcggat gatcatccga ccaacaggct
    11221 attactcaaa cgccagctaa atctattagg atatgatgtt gatgaagcca ctgatggtgt
    11281 gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg cttattactg acgttaatat
    11341 gccgaatatg gatggttttg agttgactcg caaactccgt gagcaaaatt cttccttacc
    11401 catctggggg cttacagcca acgcacaggc taacgaacgt gaaaaagggt taagttgcgg
    11461 catgaactta tgtttgttca aaccgttgac cctggatgta ctgaaaacac atttaagtca
    11521 gttacaccag gttgcgcata ttgcacctca gtatcgccac cttgatatcg aagccctgaa
    11581 aaataatacg gcgaacgatc tacaactgat gcaggagatt ctcatgactt tccagcatga
    11641 aacgcataaa gatctacccg ctgcgtttca agcactagaa gctggcgata acagaacttt
    11701 ccatcagtgt attcatcgca tccacggtgc ggctaacatc ctgaatttgc aaaagttgat
    11761 taatattagc catcagttag aaataacacc tgtttcagat gacagtaagc ctgaaattct
    11821 tcagttgctg aactctgtaa aagaacacat tgcagagctg gaccaggaga ttgctgtttt
    11881 ctgtcagaaa aataactaaa tagcggctcc cacaatgttc taatgtggga gctatttacc
    11941 agcacatctt acatttatga tgagaactcc tggcgaattt gttccccatg ctggtcgagc
    12001 gttgccgctc ccggcataac atgcgggtcc gcgcagccgc tgatttttat cggattaccc
    12061 ggcatcatta ttcctccggc ttcaatcaac atatttctcg cctgagtttg tggcaaatta
    12121 atggcctcag ccacacttaa taacggcgcg acgggcacac caacttcatg tattttggct
    12181 aaccaaactt cagctgcctg cgtttttaac gttcgctcaa tatattgttt aagaatagcc
    12241 tggttttgta cgcgtaaaat attgctgcta aatcggggat cattaaccag ttccgtaagc
    12301 tccagtgcct ggcataacgc agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc
    12361 ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg gatggcgatt tcccaggcgt
    12421 tgtggtgact tccctgtcgc aatatatgcc atcagaccat gctccagaaa actcagcgtg
    12481 gcatcaaaca tcgctatatc gacatgcgcc cctctctggc tcttttcgcg gccataaagt
    12541 gcactcacta ttccgctgaa taaatagaca ccgccgcata gatccgcaag agaggtacca
    12601 acacgcactg gcggagcatc agggtaccct gtttccatca ttatcccact cattgcctga
    12661 atgatggtat cgtaggcagg agcatctttt agcggaccgg tatgtccgaa acctgacgat
    12721 gaagcatata tgaggcgcgg gttgatttct tgtaaccttt cccatgaaaa ccccagtttt
    12781 tccattgtac ctgggcgaaa attctcagct aatacatcag cttgtttgag catatttata
    12841 aatatacttt tatcgtgatc attctttaaa tcaagaacca cactctcttt gccatgatta
    12901 ataaaactgt aatagagtga ctgtccatcc acatagggac caaatgtgcg ggtatcatca
    12961 ccatgacctg gtggctcaac tttaattacc cttgcgccca tattacaaag aagttgagtt
    13021 ccgaaaggtc cattaaggac atgtgtcata tcgataacta ataagccttc aaacggccct
    13081 ttgctttcat tatttgtcat ttgcttatgc cttgaaaaaa gccgaaggtg aaaagacact
    13141 tcggcattta tgtaaaatca ataacatgat taattatgaa accagtcgac tgacataaat
    13201 ccacaaggga gccgtgacga caaagcccag tacgctcaca gccaatgacg ctgtaccagt
    13261 acgggtgtag acattaaacc ggctggcaat aatgattccg gagaatgccg gcggtaatgc
    13321 gcctgccagt accatcatct gcaaatgttc gctgttcaaa tgacatgcca taccaacgag
    13381 aagcagcgcc agtggcatca gaatcaactt caggaaggtg ttataagcaa tttcggcact
    13441 gaactcgaat ttatgtgcag ccagagtcaa ccccgcagcg aataccgcta cccctgagtt
    13501 agccttcgca atcagattaa aggtcgggtc ccatgctgcc ggaattttta ccccaaccag
    13561 caccaggatc gttgccagaa caggtgccca tactactggc tcctttgccg cagaaattaa
    13621 tgcgctcaga ttactattct tcttaccatc cgctcctgag gaaggattca gcaaatacag
    13681 accaatagga atagtaattg cgttaacaat aatagaaata attgctacca ctaaacctgt
    13741 tgatacggaa tcaccataaa taggatcgag aactgcaaac cccaggaatc caatggtagg
    13801 tgaacccgca attaatgcac atacagcggc ttctgcatgg gtacgtttaa aaaatttgta
    13861 gcaaccaaac caggagaaaa agaaacatcc gacaataacc acaagtgata caagggtcag
    13921 acgagtgtcc gcaaaaatca tttccctgtt tgcccgggta atagaaacaa atagagccgc
    13981 aggaagagcg tagtttaata ccagtttatt aaatgcgcgg gcttgatctt ctgaaaaagt
    14041 ttctcgtctg ccgctaaaat aacccaacag catgattacg ataataggca ataaatcgcc
    14101 aataaaaaat gttagcataa tagtatttcc gataagggtc ccctcaccat cgcataatga
    14161 gttggcaaga ggagacgatg tcagtattta attaccagcg acttgttttg ggttaagttt
    14221 ggtaatatgg ccactttcag ttcctgcagc agggtcgatg accacattaa taatggtcgg
    14281 tttgcgcgac tgaataccgg tggttaaagc atgacgaagt tcatctgtcg tggtgacgtt
    14341 atagccaacg ccacgaaacg catccattaa tttgtcatac cttgcatggt gcaacagatc
    14401 cgttggtgat ggtgcaccag cgccactgag atcaacaccg tctcctctgt agatgccgcc
    14461 attattaaaa ataacgatcg tcaccggcag gttatatcga caaatcgttt caatttccat
    14521 cccactgaaa ccaaaagcac tatcaccttc aatggcgaca accggagaac cagaagtcac
    14581 gctagcaccg atggcatagc ccataccgat gcccatgaca ccccaggtac cacaatccag
    14641 acgacgacgt ggtttataca tatcaataat atttcgtgca ttatccaggg tatttgcacc
    14701 ttcattaact aaataaatat cctggttctc gcgcaatacg tcgcgcacag cacttaatgc
    14761 attaaagtaa tttaatggtt gagtatctgt acttaatttt tcatgcattt tttgtgcatt
    14821 ttgctgcttg tggatattta aaatatcgcg ccataccagt ggagtcgtaa atgtgttttg
    14881 tttcagttct gccagcatac cttgcatact ggatgcaata tcaccaacga ctggcacagc
    14941 aatggggcgg ttgctgtcaa tttcctgcgg ttcaatatcc agttgaataa actgtgtatc
    15001 tgccgcccat ccttttttac cgtgtgccaa taaccaattc agtcgtgcac caacaagcat
    15061 gacaacgtca gcatttgcca gggcaaacga acgcgcagct gccgcagaaa gtggatgcgt
    15121 atcttcaagg atccctttcg ccatagacat tggcaggaat ggaatctgag cactttcaat
    15181 aaattcacga agctgttcat cagcttgtga atacgccgcg cctttgccaa ggataattaa
    15241 tggccgttca gcttttgcta aaagcgaaat tgcgctagtg actgacttcg ggcatggcaa
    15301 taatgctggc gacggatttt caactttaac aatcgtggtt aacgcttcgt ctttttccat
    15361 cgtcgcggcc aggacatttg ctggcaaatc aagataaact ccgccagggc gacccgatac
    15421 agagacccgg atagcgcgtg ccaatgcaat gccaagatcc tgcggctgat taacgcgaaa
    15481 tgctgctttg gcatacggtt ttgccgcatt catttggtcc agctcttcat aatcaccttg
    15541 ctgtaggtcg acgatcgcgc ggtcgctgga gccgctaatc atgatcatcg gaaaaccatt
    15601 taccgttgcg ttggccaatg cggtcaaacc attgaggaat cctggcgcag aaactgtcag
    15661 gcagatcccc ggtttttggg taagaaaacc gcttgccgca gcggcatagc ctgccgactg
    15721 ctcatgacga aaaccaatat aacgaatgcc ttccgcctgg gcatggcgtg ccatatccgt
    15781 cacaggaata cctacaacac cataaatagt gtcaatatta ttctgtttta atgcttcaac
    15841 gatgatatgc ataccatctg tcatttgaag ttgatctgac atgattaata acctcgaaat
    15901 ttttataaat tgggagccag aagcccccgt taatatcaga tggcgtggtt ttgcttcatt
    15961 gcagcaattt catcgtcgct ataacccagc tcctgcaata cagcagcggt atgttcacct
    16021 aatagcggcg cagctttaat atccggcgta aaggcagaga atttcattgg acaaccaacg
    16081 gtcagatatt ttccacgcaa cggttgttcc acttcaacaa cactgccact ttggcgcaaa
    16141 gagggatcaa gtgaaatttc tttcatactt aaaaccggtg cacaaggaat atcaaactga
    16201 gtcaaatagg ccaccgcttc atgtttatca atagtgacag tgtatttttc gatttcagca
    16261 aaaatatcga aaatatgtgg ctgtcgggca tgggctgtac tgtatgccga atcggtaatc
    16321 cattctggtt tgccgatggc tttacaggtg ttttcccagt tttgctcctg aatagtgaaa
    16381 taaatatagg cgttaggatc ggtttcccag cctttacatt tcaggatcca gccaggctga
    16441 ccgccaccac ctgcattacc accgcgggga actgcatcac caaatgtacc attcggatac
    16501 tgcgggtatt cttccagata acccaattta tcgagacgct gctggtcacg taatttcacg
    16561 cggcaaaggt tcaatacggc atcctgcatt gacatggtga ctcgttgccc acgccccgtt
    16621 ttttcgcgat gcagcaaagc agcaagtaaa ccgatcagca aatgcattcc ggtgttgctg
    16681 tcacccaacg ctgcagcgct taccagcggc ggaccatccc aaaaacccgt agtggatgcc
    16741 gcgccacccg ctgcctgagc aacgttttca taggctttta cattcacata aggcgaacac
    16801 tcatcaaacc ctttgatcga accaaaaatc agacgtggat tgatttcttg aatatgctcc
    16861 caggtgaagc ccatgtgatc aatggcccct ggatgaaagt tctcgactaa gatatcagct
    16921 tcgcggatca gcttttccat tacctctttg ccttccgctg ttttggtatt taactcaata
    16981 gaacgtttgt tactgttaag catggtgaag taaagcgcat cgatatcagg aatatctcgc
    17041 agttggtgac gcgttacgtc accaacgccg ggacgttcaa ttttaataac gtcagcgcca
    17101 aaccaggcca gcatttgagt acaagatggg ccagattgca caccggtgaa atcgagaact
    17161 ttaattcctt gaagtggagt tgacatagca atacctatta gttaattaaa tatgtgttaa
    17221 tttagcgttt atttaaggca taacgccctc tataaataat ctgcaaaata tcttcgtagc
    17281 ggcactcgat atttttaaat tcctgtatca agtactgaaa attatttcat cgccacttcc
    17341 aattcctttc tgcatggtaa gtagtgagga cgaaaagaag aatatgataa atatttattc
    17401 gtggtaatca gagagagtcg cgacatttgt gatgttattc ttacaactgt aagaagcgcc
    17461 ggtgatttac aagtaagagt atgattacgc tttttaaagc gtaccataaa gcataacata
    17521 ataaatgatt tgtgatgaca ctggattatt gcggtttact ttgaggaaaa taaaatgcgt
    17581 gtttatagaa ccgcgagcgc caaataattt tatgaccaca acctgtcgta ttgtggtcat
    17641 aaaaaattgt cggctggagg gagttaaatc ctttacctca atatactgcc tgtcagcatt
    17701 agttcacaaa cagtgcttcg ctatcgacga tgattccatc ttccgcacct ttaagcgcca
    17761 ggtttgcctc gtagtattgc tttttatcaa gcagtttctg cgcatcagca agtgcattac
    17821 gagtctgttt aagcggcatc agatattggt tttccattac gccaacaccg gccaggcgaa
    17881 gctcttccat tgcgcccttt ttatcccctt tcgccatttt ttcattggca atttttatcg
    17941 cggcttcttt ttcgggtgtt gctacataac tctcagaaat tccgacagat gcattaatga
    18001 caatatattg gtcatcattg agattggtct ttttacctgg cttagcgaac tttgcccatt
    18061 cggtactgtc atctgacagc aaagcggaag cttcattggt cagttctttt gccttttccg
    18121 gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca tataaacctt
    18181 gttcagagat acgctctgct gctgccgttt tctgggtttg ctgagtttgc tgttgtgccg
    18241 ctaccggtgc attatcagca gcccatacag ttgaagatgc cagaattgct gtgaccatcg
    18301 tagccataat taaacgtttc atacttacct cattctttaa cagttttaga aaacgccgcc
    18361 atcatattgg gtgagcatga aatgagaaat cagagagcaa cggtcatttt gaaagggtta
    18421 tcttacaatt gtagttatta tcgcaaagcg gcatttagtc ttacagttgt aggaatatgc
    18481 ttcctgtcgc agagaactat tcttatatac cacaaagaga tgtcatttat tatattgatg
    18541 taaatattga ttaattatat gtaaatacag ccataacatc gtatggttct cagtgaacca
    18601 ttttcaggat aactttctat gaaagtaaac ttaatacttt tcagcttatt tttattggtc
    18661 tctattatgg catgcaatgt ttttgcattt tccatttctg gtggtgtaag tgagaggagc
    18721 tataaagaga ctgaaaaaac atcagcgatg acaaccacac actctacaaa acttcagcca
    18781 tcacaggcga ttttgtttaa gatgagagaa gatgcgccac cattaaacct cacagaagaa
    18841 atcacgccta cttatccgac aaaggcgaat tatcttattc atcctgtgcg atagcgtctg
    18901 tcacatggca ggtgaacaat catgtctgat gctgatgtga acacctgccc aaatgagcca
    18961 tcctgaccca ctatcgcctg ccatacccca ccataaagcg aatcatcaag ccacaagccg
    19021 gggcaagaaa cataaatatc cccgtcacca gttcattacc gccagttaac cacatggtga
    19081 agtaataaat gagtgcagcg aggatcacca tgccgaagaa agaagagacc gccgatacca
    19141 cgttcagtac ctgaccgatg tagccgctta cgcagacaat acacagggcg agaaacatcc
    19201 ataaataaat catagctcaa cctcctgccg cgctaaaaaa cctgtaactt cagtcacttt
    19261 aatagtaaga caatccttaa cctctggcaa tttttgctaa gcatcaatga aaacagcacg
    19321 ttaaatgcaa cgctattgcg tgcggaaaat tttttacttt gcacgattaa taatcagtag
    19381 ctgaaagcag tcagccagtg ataaagtaga gccaccgcat tcatgctcct cgtggttatg
    19441 tcctgactag tctttacact ctttacagga accattgtcg tacatgatgg cccaaccaat
    19501 tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa caattcgtat ctacagaagg
    19561 taactatgtt tccacaatgc aaattttccc gcgagtttct acatcctcgc tactggctca
    19621 catggtttgg gcttggtgta ctctggcttt gggtacagct tccttatcct gttctctgct
    19681 ttctcggcac gcgtattggc gcaatggcgc gaccattcct gaaacgtcgt gaatctatcg
    19741 cccgtaaaaa cctggaactt tgtttcccgc agcattctgc ggaagaacgc gagaagatga
    19801 ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga aaccggcatg gcatggttct
    19861 ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg gttggataac cctaaacgcg
    19921 cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg ctggaactgg
    19981 gcggccgcgt gatgggactg tgccaaccaa tgatggctac ctatcgtcca cataataatc
    20041 agctgatgga atgggtgcag acccgtgggc gcatgcgctc taacaaagcg atgatcggca
    20101 gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg tgaagcggta tggtttgctc
    20161 ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc gttctttgcg gtggaaaatg
    20221 tcgccacaac caatggcacc tatgttctct cccgtctctc tggcgcagcc atgttgaccg
    20281 taacgatggt aagaaaagcg gattacagcg gatatcgttt gttcatcacc ccagagatgg
    20341 aaggctaccc gacagatgaa aatcaagccg ctgcctatat gaacaagatt atcgagaaag
    20401 agatcatgcg cgcaccggag cagtacctct ggatccaccg tcgctttaaa acgcgcccgg
    20461 tgggagaatc gtcgttgtac atttaacaaa ctgctcttac tctttccctc ctcaaatcgg
    20521 ccataataac ctcatggttt ttatggcttt tttcttaagg ttttttcctt cgctgaatat
    20581 ctaattatta ccgtcagaaa ttaaactgtc gccgatatga gacacttgta agttactaaa
    20641 agtaaataag aaaatgcctc ttgtctcgtt tcagttttag gcgtacatta acgccgtctt
    20701 gagcagttta gctcagaatt ctggggtgat gcccacggta aaatacaggg cttttaaggt
    20761 cgctttgccc ggtgtcaact cactgtatca ggtgtaatga agtcattcag gcgtaacagt
    20821 aattacgcgg agagatgtaa agtgaaatat ttctttatgg gcatttcttt tatggtcatc
    20881 gtttgggccg gtacttttgc cctgatgatc taaaagcaaa ctgtgcaatg aaaaacagga
    20941 gccatcaggc tcctgtttgc atttctgacg acggtgttta ttccgcgttt tcgtgaatat
    21001 gtttgctgct ggcgggtagt aaaccgtcgg cacggaacat cgctttaatc cctctgatgg
    21061 cctgacgaat acggtcgcgg ttttcaatca gtgcaaagcg aacatgcgta tcaccatagt
    21121 cgccaaagcc aatccccggc gagacacaaa ccttcgcttc gttaaggagc ttcttggcaa
    21181 attccagcga tcccatggcc gcatatggtt ccgggatttt cgcccagaca tacatcgaag
    21241 ccttcggcat ttcgaccatc cagcccgctt catgcagccc tttaaccaat acatcacggc
    21301 ggcgtttgta ctgttcggta atgtcgcgca cgcactgttg atcgccctcg agtgccgcaa
    21361 tcgccgccac ctgcaacggc gtaaaagtgc cgtaatcgtg atagcttttg attcgcgcca
    21421 gggcactgac cagcgttttg ttgccgacca taaagccgat acgccagccc gccatgttgt
    21481 agcttttcga cagcgtaaag aactcgaccg ccacatcgcg tgcacccggc acctgcataa
    21541 ttgacggcgc tttccagcca tcgtagacga tatcggcata ggccaggtca tggaccacca
    21601 gcacatcgta gcgtttcgcc agcgccacaa ccttttcgaa gaactccagt tccacgcatt
    21661 gcgcggttgg gttcgacggg aagccgagga tcatcatctt cggtttcgga taactttcac
    21721 gaatggcgcg ctccagttcg ttgaagaaat cgacaccttc caccagcggc actgagcgta
    21781 cctgcgcccc ggcaatcacc gcgccataaa tatggatcgg gtaacttgga ttcggcacca
    21841 gcaccgtgtc accatgatcc agcgtcgcca gcatcagatg cgccaggccc tctttcgaac
    21901 caatagtgac gatggcttct gattccgggt cgatttcaac gtcgtagcga tcctgatacc
    21961 agcgggaaat ggcgcgacgt aaccgcggaa tgccgcgtga agtggagtaa ccatgcgtgt
    22021 ccgggcgctg ggccacagta cataattttt cgacgatatg cggcggagtc gcaccgtccg
    22081 ggttacccat gctgaaatcg ataatatctt cgccgcgccg acgcgcagcc attttcagtt
    22141 cagcggtgat gttaaaaacg tagggcggga gacgatcaat gcgcgtaaag cgacgttcag
    22201 ggcgagtgtc agccataatt tcctcagatt aacgttagcg cccggaccgt ccgagcgacg
    22261 ctgccacaat ggtggctgtt ttgaaaatag cctgattaat ttctgtctgt ccagcgtttt
    22321 agaacatttt tatcaatggc tttaaatgag gaactccact tcaaaaacag gaaatttttc
    22381 tacgctaaca ag
//
LOCUS       DAZPDU010000058        22241 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZPDU010000058 DAZPDU010000000
VERSION     DAZPDU010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22241
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.058"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..1463
                     /gene="dgcJ"
                     /locus_tag="ACHURS_002015"
     CDS             <1..1463
                     /gene="dgcJ"
                     /locus_tag="ACHURS_002015"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFR1237756.1"
                     /translation="FIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQKADSSFLYDK
                     YQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHKYPGLRGTLQT
                     ASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEP
                     VNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYV
                     AGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQY
                     VSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHFRLYQNVSREN
                     ISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAI
                     TLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRLQHIAPQKEIG
                     FSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            1506..2009
                     /gene="yeaK"
                     /locus_tag="ACHURS_002016"
     CDS             1506..2009
                     /gene="yeaK"
                     /locus_tag="ACHURS_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFR1237757.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     LNFRRTA"
     gene            complement(2010..2114)
                     /gene="yoaI"
                     /locus_tag="ACHURS_002017"
     CDS             complement(2010..2114)
                     /gene="yoaI"
                     /locus_tag="ACHURS_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFR1237758.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            2284..2730
                     /locus_tag="ACHURS_002018"
     CDS             2284..2730
                     /locus_tag="ACHURS_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFR1237759.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2687..3508)
                     /gene="nimR"
                     /locus_tag="ACHURS_002019"
     CDS             complement(2687..3508)
                     /gene="nimR"
                     /locus_tag="ACHURS_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFR1237760.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            3605..4786
                     /gene="nimT"
                     /locus_tag="ACHURS_002020"
     CDS             3605..4786
                     /gene="nimT"
                     /locus_tag="ACHURS_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFR1237761.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFIGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLATPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMIVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLIYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            4841..5188
                     /locus_tag="ACHURS_002021"
     CDS             4841..5188
                     /locus_tag="ACHURS_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFR1237762.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(5210..5464)
                     /gene="yoaF"
                     /locus_tag="ACHURS_002022"
     CDS             complement(5210..5464)
                     /gene="yoaF"
                     /locus_tag="ACHURS_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFR1237763.1"
                     /translation="MKIISFALPCLLVLAGCSTSSQPEVPKPPQIGMANPASVYCQQK
                     GGTLIPVSTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            5647..6672
                     /gene="dgcP"
                     /locus_tag="ACHURS_002023"
     CDS             5647..6672
                     /gene="dgcP"
                     /locus_tag="ACHURS_002023"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFR1237764.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGISTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNPRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHSAPHC"
     gene            6705..6803
                     /locus_tag="ACHURS_002024"
     CDS             6705..6803
                     /locus_tag="ACHURS_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFR1237765.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            6806..6880
                     /gene="yoaJ"
                     /locus_tag="ACHURS_002025"
     CDS             6806..6880
                     /gene="yoaJ"
                     /locus_tag="ACHURS_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFR1237766.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(6939..7187)
                     /locus_tag="ACHURS_002026"
     CDS             complement(6939..7187)
                     /locus_tag="ACHURS_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFR1237767.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(7335..7517)
                     /locus_tag="ACHURS_002027"
     CDS             complement(7335..7517)
                     /locus_tag="ACHURS_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFR1237768.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(7521..7880)
                     /gene="yeaR"
                     /locus_tag="ACHURS_002028"
     CDS             complement(7521..7880)
                     /gene="yeaR"
                     /locus_tag="ACHURS_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFR1237769.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFEVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(8053..8691)
                     /gene="leuE"
                     /locus_tag="ACHURS_002029"
     CDS             complement(8053..8691)
                     /gene="leuE"
                     /locus_tag="ACHURS_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFR1237770.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(8818..9741)
                     /gene="dmlR"
                     /locus_tag="ACHURS_002030"
     CDS             complement(8818..9741)
                     /gene="dmlR"
                     /locus_tag="ACHURS_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFR1237771.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITIEFLRQYFAEHYPNFSLEHA"
     gene            9844..10929
                     /gene="dmlA"
                     /locus_tag="ACHURS_002031"
     CDS             9844..10929
                     /gene="dmlA"
                     /locus_tag="ACHURS_002031"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFR1237772.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFDLSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGSATTPQVADAICKIILR"
     gene            11180..12790
                     /gene="yeaV"
                     /locus_tag="ACHURS_002032"
     CDS             11180..12790
                     /gene="yeaV"
                     /locus_tag="ACHURS_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFR1237773.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANTIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISTWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            12822..13946
                     /gene="yeaW"
                     /locus_tag="ACHURS_002033"
     CDS             12822..13946
                     /gene="yeaW"
                     /locus_tag="ACHURS_002033"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFR1237774.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            14002..14967
                     /gene="yeaX"
                     /locus_tag="ACHURS_002034"
     CDS             14002..14967
                     /gene="yeaX"
                     /locus_tag="ACHURS_002034"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFR1237775.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGISITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(15021..16136)
                     /gene="rnd"
                     /locus_tag="ACHURS_002035"
     CDS             complement(15021..16136)
                     /gene="rnd"
                     /locus_tag="ACHURS_002035"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFR1237776.1"
                     /translation="MITTDDALASLCEAVRVFPAIAQDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(16218..17903)
                     /gene="fadD"
                     /locus_tag="ACHURS_002036"
     CDS             complement(16218..17903)
                     /gene="fadD"
                     /locus_tag="ACHURS_002036"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFR1237777.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLIDMFEQSVVRYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCCRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(18108..18689)
                     /gene="yeaY"
                     /locus_tag="ACHURS_002037"
     CDS             complement(18108..18689)
                     /gene="yeaY"
                     /locus_tag="ACHURS_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFR1237778.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MNAPDLYVGQEARFGGKVVVVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGTVDGKVNNAPYKFMVMQVTGYKRWHITQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVETIVTE"
     gene            complement(18729..19424)
                     /gene="tsaB"
                     /locus_tag="ACHURS_002038"
     CDS             complement(18729..19424)
                     /gene="tsaB"
                     /locus_tag="ACHURS_002038"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFR1237779.1"
                     /translation="MRILAIDTATEVCSVALWNDGNVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLRKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(19482..21394)
                     /locus_tag="ACHURS_002039"
                     /pseudo
     CDS             complement(19482..21394)
                     /locus_tag="ACHURS_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
     gene            21526..21870
                     /locus_tag="ACHURS_002040"
     CDS             21526..21870
                     /locus_tag="ACHURS_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFR1237780.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         5450 a         5699 c         5401 g         5691 t
ORIGIN      
        1 attttatcgc cgcaagtgtc attgtgctga catcttcctt ccttattttt gaacttgtcg
       61 ccagcgacag agcaatgagt gcctatctgc gctatatcgt gcagaaagca gactcctcct
      121 ttctttatga taagtatcag aatcagagta ttgccgcgca tgtgatgcgc gctctcgctg
      181 ctgagcagtc ggaagtgtcg ccagaacagc ggcgcgccat ctgcgaggct tttgagtctg
      241 ccaataatac ccatggctta aacctgactg cccataaata cccgggctta cgcggcacac
      301 tacaaaccgc atccactgac tgcgacacaa ttgtggaagc tgcagcacta ttacccgctt
      361 ttgatcaggc agtggaaggc aaccgccacc aggatgatta cggttcaggt cttgggatgg
      421 ccgaagagaa atttcactat tatctcgatc tcaatgaccg ctatgtctat ttttatgagc
      481 cggttaatgt tgaatacttt gcgatgaata actggtcctt cctgcagtca ggaagtattg
      541 gcatcgatcg caaagatatt gaaaaggtat ttaccgggcg taccgtattg tcgagcattt
      601 accaggatca gcgtactaaa cagaacgtga tgagtttgct gacgccggta tatgtcgcag
      661 ggcagctaaa agggattgtg ctgctggata ttaacaaaaa caatttgcgg aatatctttt
      721 acactcatga ccgccctctc ctctggcgtt ttctcaatgt cacgctaacc gataccgatt
      781 cggggcgcga cattatcatc aaccagagcg aagataatct gttccagtat gtcagttacg
      841 tccatgactt accgggcggc attcgtgtct cgttatccat tgatattctt tactttatca
      901 cgtcttcgtg gaaaagcgtt ctgttctgga ttttgacggc gttaattttg ctgaatatgg
      961 tccgaatgca cttccgttta taccaaaatg tgtcgcgaga aaatattagt gatgcgatga
     1021 ctggactgta taatcgcaaa attttaaccc ctgaactgga gcagcggttg cagaaactgg
     1081 tgcaatccgg ttcttcggtg atgtttattg caattgacat ggacaagtta aagcaaataa
     1141 atgacaccct cggtcatcag gagggggatt tagcgattac gttattagct caggcgatta
     1201 aacaatcgat tcgtaaaagt gattatgcca tccgactcgg tggcgatgaa ttctgcatca
     1261 ttcttgtcga ttcgacgccg caaattgcag cacaactgcc tgaacgtatc gaaaaacgtc
     1321 tgcaacatat cgcgccgcag aaagaaatcg gcttctcttc cggtatttac gcgatgaaag
     1381 aaaacgatac gttacatgat gcgtataaag cttccgatga gcgtttatat gtcaataagc
     1441 agaacaaaaa cagccgttca tgataacctt ctgtggttgt ttgcttgtaa tctcaggagc
     1501 gtgaaatgac tgaaatggct aaaggaagcg tgacgcatca gcggttaatc gctttattat
     1561 cacaagaagg tgctaacttc cgcgtcgtta cccatgaagc ggtaggtaaa tgtgaagcgg
     1621 tgtcagaaat tcgtggcacc gctttaggcc agggagctaa ggcactggta tgtaaagtca
     1681 aaggcaatgg cgtaaatcaa catgttctgg cgatcctcgc tgccgatcaa caggccgatc
     1741 tgagccaact tgccagccat ataggcggat tacgcgcttc gttagccagc cctgctgaag
     1801 tcgatgaact cacaggctgt gtcttcggcg cgatcccccc tttcagcttc catccaaaac
     1861 tcaaactggt tgccgaccct ctactcttcg aacgatttga tgaaatcgcc ttcaatgcag
     1921 ggatgctgga taaatccgtt attttgaaaa ccacggatta tttgcgcatt gcgcaaccag
     1981 aactgctcaa tttccgccgc actgcgtaac tagcctgtcc gttcaataag cagaacggac
     2041 aaaaccagca caacagcaat ggcaaaaaac gatgacgtga taatcagtgt ctcgacgaac
     2101 atttgatcgt tcatagcatg cccccgggag atagctgttt tcacgttatt gttagcgtgc
     2161 acaaatggca gtttgatgac aattcgcctg attttttatg caaaaaacgt aaagattttc
     2221 tacttccttc ctgcagcaaa cgtaaagtaa gcaggcttat tattttttgg caaggaaacc
     2281 acgatgtttg atgtcacttt gctgatcctg ctcgggttag ctgcgctggg ctttatcagt
     2341 cataacacca ctgtcgccgt ttcaattctg gtgttgatca ttgtccgcgt cacaccgtta
     2401 agcacctttt tcccgtggat tgaaaaacag gggctaagta tcgggataat cattctgacg
     2461 attggcgtta tggcccccat cgccagcggg acgctaccgc cctcaacctt gattcactcg
     2521 tttctgaact ggaaatcgct ggtggcaatt gcagtaggcg tgattgtctc ctggctgggt
     2581 gggcgcggcg tgaccttaat gggcagccag ccgcaactgg tcgccgggct gctggtaggc
     2641 actgttttag gcgtggcgct gtttcgcggc gtaccggtcg gcccgcttat tgccgcgggt
     2701 ctggtttcgc tgattgtggg gaaacagtag ttaatcccgc aatatatcgg cctggcgttt
     2761 gccccaggcc tttcttaaac atagtaataa aggccgttgt tgaatcatac cccagcgtat
     2821 gagcaacttt ctgtacagta tcgcctttca ctaatccctg aagtgccata atcagctgta
     2881 actgctgacg ccactggcga aaactcaacc ccgtctcttt tactatcagt cgcgcaagat
     2941 tgcgttcact catcgcaaaa aagccagccc attgccctaa tgacccccac tcgacgggcc
     3001 ctttcgccat catctccacc atcgtgcgga ttttaggatg agaagaaacg ggcaggtgca
     3061 gctgttgttg tggctgctga gggagttcat caaacaggac ctgaattaat ctgcgggtca
     3121 tcggctcagc acgctgagtc gtcgtcctgt ttgccagggt taaaatcaat tcccggcata
     3181 agggcgatat tttcaatgtg caacatgttg tcggcattgt cactgcgctg ggttcgataa
     3241 acagaaaaca gagttcagcg ttagctgtca cttgattgct atgttccacc ccgcccggta
     3301 tccaaaccgc atattgcggc ggcaccatcc acaaagcatt ttccaccgta caggtaattg
     3361 caccatgtaa tgctagtatc agctgtcctt tgcggtgctg atgtacagga ctggttaact
     3421 catccgttcc ggcgtgaata caaaatgcga ccgcggcttc atggtgtcgg tcgggttcat
     3481 agccattgag attcaacctg tgcatcattt tgtccgaatt tagcgataat ttgtcatttt
     3541 agcttgattc aacataacaa taaaaacggt aaggtacagc ctcgtttgta acaatgagaa
     3601 gcatatgacc tgttcaactt cattaagcgg caaaaacagg attgtcctta tcgctggcat
     3661 tctgatgatt gccacaacat tacgcgtcac ctttatcggc gcagcaccgt tactggatac
     3721 gattcgttcc gcttactcgc tgacgacagc gcaaaccggc ttattgacca ccctgccatt
     3781 attggccttt gcgctaatct cacctttggc caccccggta gcgcgacgtt ttggtatgga
     3841 acgtagcctg tttgccgcgt tacttttgat ctgtgctggt atcgcaattc gctctctccc
     3901 ttcgccttac ttattatttg gcggtacagc ggtcattggc ggtgggattg cattaggcaa
     3961 tgtcttactg ccaggattaa ttaaacgcga tttccctcat tccgtcgcca gacttaccgg
     4021 cgcatattcc ctgacaatgg gagctgcagc ggcactggga tcggctatga tagtgccgct
     4081 ggctttgaac ggttttggct ggcaaggcgc gttgctcatg ctgatgtgtt ttcctctgct
     4141 ggctcttttt ttatggctgc cacagtggcg aagccaacaa catgcaaatt tgagtacctc
     4201 gcgcgcctta catactcggg gtatctggcg ttcgccgctt gcctggcagg taacattgtt
     4261 tcttgggatc aactcactga tctattacgt gattattggc tggcttccgg cgatcctcat
     4321 cagtcacggc tatagcgaag cacaggcggg ttcactgcat ggtttgctgc aactagccac
     4381 agcagctccc ggtttgctga tccctctttt cttacatcat gtgaaagatc agcgtggtat
     4441 tgcagcgttc gttgccttga tgtgcgcagt gggcgctgtt gggctctgct ttatgccagc
     4501 gcacgcgatc acctggactc tgcttttcgg ttttggttcc ggcgcaacaa tgatactggg
     4561 gttgacgttc attggtcttc gggcaagttc tgcgcatcag gcggcggcac tctcggggat
     4621 ggcacaatcc gtcgggtatt tgctggcagc ctgtgggccg ccgctaatgg gtaaaataca
     4681 cgatgctaac ggtgactggt ccgtaccact gatgggtgtt gcaatacttt cactactgat
     4741 ggcgattttc ggactttgcg ccgggagaga caaagaaatt cgctaatatc cggtgctata
     4801 gtgacgtgac aaatcatgcg tgaaagggag aacaaacacg atgaatattc agtgcaaacg
     4861 cgtttatgat ccggctgaac agagcgatgg ttatcgcgta ctggtcgacc gcctctggcc
     4921 gcgcggtatc aaaaaaaccg atttagccct tgatgagtgg gataaagaaa tcacgccgtc
     4981 aacggaactg cgcaaagcct ttcacggcga agtcgtcgat tttgcaacct ttcgcgagca
     5041 atatcttgca gaactggcgc aacacgagca agaaggaaag cggctggcgg acatcgccaa
     5101 aaaacagccg ctgaccctgc tctacgcagc aaaaaacacc acgcagaacc atgcgctggt
     5161 gctggccgac tggctacgta gcttgtgatt ttagtacagc atccggcggt tatttttcac
     5221 cagccggatg gtcccgtcgc cacaatgccc attcatcaat cgtttcaccg cccggtaatt
     5281 tgcaattgtt gctgacccct tgcgctgtcg acactggaat gagcgtcccg cccttctgct
     5341 ggcaatagac cgacgccgga tttgccatac caatctgcgg cggtttaggt acttctggct
     5401 gagaagaggt tgaacaacca gccaggacca gcaagcaagg caaagcaaaa ctgataattt
     5461 tcatttattg atctcactca tttatctacg cttagagtat cgcggtatcg ttttgttttg
     5521 cagcactatt tttattacat tcactcaaaa catattacgt cttgtttcat ctttgttggt
     5581 gatgttttat catgcctgca aagattaaat aatcagcatt tacccgccgt atcctggagt
     5641 tgtttcgtgt cagatcagat tatcgcccgc gtctcgcaat cccttgccaa agaacagtca
     5701 ctggaaagtc tggtccgaca gcttctggag atgctggaaa tggtcactga tatggaatca
     5761 acctacctga ccaaagtgga tgtcgaagcg cgcctgcagc atattatgtt tgcccgtaac
     5821 agccagaaaa tgcacatccc ggagaatttt accgtctcgt gggattactc gttatgcaaa
     5881 cgcgccattg atgaaaactg ctttttcagc gatgaagtcc ctgaccgttg gggcgactgt
     5941 attgcggcac gcaatcttgg catctccaca tttctgagca caccaattca cttaccggat
     6001 ggatcattct atggcacgct ttgcgccgcc agcagtgaga agcgccagtg gagtgaacgc
     6061 gcggaacagg ttttacagtt attcgccgga ctgattgcac aatatattca aaaagaggca
     6121 ctggttgaac agttgcgcga agccaatgct gcgctgattg cgcaatcgta taccgactcg
     6181 ttaaccgggt tgccgaatcc gcgggcgatt tttgaaaatc tgacgacgct gttttctctc
     6241 gcccggcatc ttaaccataa gataatgatc gcgtttatcg atctggataa cttcaaatta
     6301 atcaatgatc gttttggtca taatagtggc gatctgtttc tcattcaggt tggcgagcgc
     6361 cttaatacgc tccagcaaaa tggcgaagtt attggtcgtc tcggcggtga tgagtttttg
     6421 gttgtttcac taaacaacga gaatgcggat atttcgtcgc tgcgagaacg cattcagcag
     6481 caaatacgtg gagaatatca cttaggtgat gttgatttgt attatcccgg tgccagtctt
     6541 ggcatagtag aagtcgatcc tgaaacaacc gatgcagaca gtgccctgca tgctgccgat
     6601 attgcgatgt atcaggagaa aaaacacaaa cagaaaacac cttttgtcgc gcattcagcg
     6661 ccacattgct gaggcgtatt cacatccttt tgattggtga taacatgcga atcggtatta
     6721 tttttccggt tgtaatcttt attacagcgg tcgtattttt agcatggttt tttattggcg
     6781 gctatgctgc cccgggggca taaagatgaa aaaaacaacg attattatga tgggtgtggc
     6841 gattattgtc gtactcggca ctgagctggg atggtggtaa cgtcacctct aaaaaatagc
     6901 aaaggctgcc tgtgtgcagc ctttgtgcaa tttaagcgtt aacttttaat cttcctgtag
     6961 ataaatagca cgacaatcgc accaataacg gcaaccacga agctgccaaa attgaagcca
     7021 tcgactttac caaagccaaa cagcgtgctg atccatccgc cgactacggc accgactatc
     7081 cccagcagga tagtcataaa gaatccacca ccatctttac ctggcatgat ccacttcgcc
     7141 agaataccgg caataagccc aaaaataatc catgacagaa tgcccattgt ttcctcactt
     7201 atctgttttg cattagcggg ttagtcgctg ataaaaagca tagcacaaca tcgggagggc
     7261 aagatttgtg acgagcatca cggaggattt ttttgcgatg gcgcagaaat tgcgccatca
     7321 acgatcagtg ataattacca accacaaaca tcatgttcgt tttccgtgtc ataagaacgc
     7381 acggtattca ccagatcttt tatcacttca gccgccactt ctggcaccag caaagtcatc
     7441 ggcgtctctg tttcataatc gacagaaacg ccattactgt tattggtgac ggtcacggta
     7501 tacgttgctt tgcccatgat tcatttcccg ttatgaatga ctttccgttg ttgcgcacct
     7561 tccatcagga cttcaggagc cacgaagaag tcaatgttga aataagtatc gtcagtcatg
     7621 gcttcaatgt tgtgccactt ttctggaggg aacacctcaa actgccccgc ttcgataagg
     7681 atcacctgat caggctctgc actgtgttca tcagcgtagc cgagatattt gaccgcccca
     7741 tgcataacgg aaaggcgtgg gtaaaccccc gggcgcgttc ctttatcaag atgacgttcg
     7801 aatattccgg caggcgcagt ttgtttattc cagaaaggcg ttgagcgcgt atgaatataa
     7861 ttctgtggga tttgcagcat ccttttccct ccttcggtga atgcgctgaa aacggtttat
     7921 tccagccgtt tcagggtacg cctgataatt tgcattttaa ataccattta ttggttactt
     7981 tttagcacca tatcagagaa aaatcaggga ggattacaag tgggaagccc gcgacaacgc
     8041 gggctgaatg tatcaggatt gcagcgtcgc cagtcgggcg gcgaaaccca cgaacatcaa
     8101 accaatcagt gagttgccaa ctttagccag tttctttttg gtacgtatgt actgcgtgac
     8161 aaaagcacca gaaataatca ggaagctcaa atagcagaaa ctcaccagtt ccagcgtcgt
     8221 cgccagaata aagaatgaaa ttcccgtatg tggggcatta acatcgataa actgtacgaa
     8281 aaacgacaca tagaacaaaa tggctttcgg attagtcagg ctcaaaatta acgcgcgttt
     8341 aaaaatggcc ccgtattggg gctcatcgga tttggcctcg ttatttttac ccttcagggt
     8401 cgcgtagaga attttactcc ccagatagag caaataaaac gcaccaagat aacgtacaat
     8461 gttgaataat atcggggtgg tcttaattaa tgtcgccact ccagcccatg ccagaaacat
     8521 caatactgca tcgccaataa atacaccgca ggccgcaaga taaccgcctt tcataccgct
     8581 actgacgcta tttttgagta caaacagggt atttggccct ggcaccaaca caataaaaat
     8641 ggccccaacc agataggtcc agtaattcag aaccccgtat tcagcgaaca cattaacctc
     8701 tttaattatc tttcgatcat gcgcgattaa aggtgaatat gctaaccaat ctgtagcggc
     8761 ttagaaagga gaaaatcagg ttttaacccg atatcaaccc gataattgaa taattaatca
     8821 ggcatgctcc agtgaaaaat tcgggtagtg ctcggcaaaa tactggcgta aaaattctat
     8881 cgttatccgc actttcgctg acgtcgccag ccttgaaaca taaacggccc agacgttcgc
     8941 tggctggtaa tattccggta gcacttgcac taaatgacca ctggcaatgt tttcgctaac
     9001 atcccaccag gagcgcagag caatcccctg cccatcgaga caccactgat ggacaatttc
     9061 tccatgatta gacgacagtg ggccggtgac tttaatcgca tggtggcctt ctttattgcg
     9121 taattgccag acaccaaaag gatgatcgcg ctctttaatg accaggcacg gcaatgcgga
     9181 aagatcggtt aaatgtttcg gcgcgccatg ttgcgcaata aattccggcg acgcgcagag
     9241 aatgcggtaa ttagtcgcca gtttgcgggc gattaaatta ggggcgatgt catcgccaat
     9301 gcgaatatcg agatcaacac cttcattgac caaatcgacc aaccggtctt ccacatcaaa
     9361 acgtaattcc agttgcggat acgccttagc cagcgctgat aacgccggag ccaccacctg
     9421 tcgtccaaaa ccaaagctgc tgataatacg cagcatcccc tgcggcacct gacgcacgtc
     9481 agaaagttcg tccatcatct gaccgacatc ctgcaaaatc cgctgcgccc attcataaat
     9541 ccgctctcct tcttcggtaa tggtgacgcg gcgggtggtg cggtgtaaca acaccacgtt
     9601 tagcgtttgc tccagcaagg cgatgcgctt gctgacgaac gccggtgaaa cgcccagttc
     9661 ttccgccacg gcggcaaaac cagcccggcg agccaccagc ataaagacgc gcaaatcgtt
     9721 cagcagcggt aaattattca tgattcgtgt tttatgtttc accagttacg gggattaatt
     9781 ccttttcagt caattatagg atggtgatgt tgtcaatttt gatggtcagg aagtgagaac
     9841 ccaatgatga aaacgatgcg tattgctgcg atcccgggag atgggattgg caaagaagtc
     9901 cttcctgaag ggattcgcgt attacaggct gccgctgagc gctggggctt cgacttgagt
     9961 tttgagcaaa tggagtgggc gagctgcgag tattacagcc atcacggtaa aatgatgccg
    10021 gacgactggc atgagcaact tagccgtttc gacgccatct attttggtgc cgtcggctgg
    10081 ccggataccg ttccggacca tatttcgttg tggggttcgc tgctgaaatt tcgtcgtgaa
    10141 ttcgaccagt acgtcaacct gcgcccggtt cgtctctttc ctggcgttcc ctgcccgctg
    10201 gcgggaaaac agcctggcga catcgatttt tacgtggtca gggaaaacac cgaaggcgaa
    10261 tattcctcgc tcggcggtag agtgaatgaa ggtacagagc atgaagtcgt cattcaggaa
    10321 tcggtattta cgcgtcgtgg tgtcgatcgc attttgcgtt atgccttcga acttgcgcaa
    10381 agccgcccac gtaagacgct aacttctgcc actaaatcaa acggtttagc catcagcatg
    10441 ccgtactggg atgagcgagt ggaagcaatg gccgagaatt acccggagat ccgctgggac
    10501 aagcagcata ttgatattct ctgcgcgcgt tttgtgatgc agccggaacg cttcgatgtg
    10561 gtggtggcgt ccaatttgtt tggcgatatc ctttccgatc ttggcccggc ctgcaccggc
    10621 accattggca ttgccccatc cgccaacctg aatccggaac gcactttccc gtcgctcttc
    10681 gagcctgtcc acggttccgc gccggatatc tacgggaaaa atattgctaa ccctatcgcc
    10741 acaatttggg ccggtgcgat gatgctcgat tttctcggca atggcgatga gcgtttccag
    10801 caagcgcata acggtattct ggcagcgatt gaagaagtga ttgctcacgg gccgaaaaca
    10861 ccggatatga aaggcagtgc caccacgcca caggttgccg acgcgatttg caaaattatt
    10921 ttgcgttaag gtcaaaccag tttatttgaa ccgcgtcact gacgcggttt ttttattcgt
    10981 tctttgcagt taataacctg cgtcatttca ccttttattg tttccgtttc gtgttttatg
    11041 gctttccgta ttcttaattg tttaatttat gtaacatgca aataatcaat ttgttaaaca
    11101 caacatacaa ctcattaccc tgcaaataat tacaactggc aaaagccaga gacgggcttt
    11161 tatgcccagg agatgactca tgatgagcaa tgttaagaaa aaagatgtgc cgctgataag
    11221 catcagcctg gtggccattc ttttcatcgc agctgcatta agccttttcc cacaacaatc
    11281 ggccgacgcg gccaacacaa tatacacttt tgttacgcgt acgttaggtt ccgccgtaca
    11341 ggtattggtt ttgctggcaa tgggactggt gatttattta gccaccagta aatacggcaa
    11401 tattcgtctt ggcgaaggaa aaccggaata cagcacgctc tcctggctgt ttatgtttat
    11461 ttgtgccggt ttaggttctt ctacgcttta ttggggggtt gctgaatggg cctattatta
    11521 tcaaacgcct ggattaaata tcgcaccacg ttcacaacag gcactcgaat ttagcgtccc
    11581 ttactccttc ttccactggg gcatcagcac ctgggcaact tatacgctgg cctcattaat
    11641 catggcttat cactttcatg tgcgtaaaaa caaaggtctg agtctttccg gcattattgc
    11701 cgcgattacc ggcgttcgcc cgcaaggccc atggggaaaa ctggttgatt tgatgtttct
    11761 gatcgccact gtcggcgcac tgactatttc ccttgttgtc accgcagcaa cctttactcg
    11821 tggactttcc gcgctgaccg gtttacccga taacttcacc gtgcaggcat ttgtgatcct
    11881 gctttccggc ggcatttttt gcctaagctc gtggattggt atcaacaacg gtttgcaacg
    11941 tctgagcaaa atggttggct ggggcgcgtt cctgctgcca ttactggtgc tgattgtcgg
    12001 tccaaccgaa tttattacca acagcatcat caatgccatc ggcctgacca cgcaaaactt
    12061 cctgcaaatg agcttattca ccgatccgct tggcgatggt tcatttaccc gcaactggac
    12121 cgttttctac tggctgtggt ggatctcata cacccctggc gtagcaatgt ttgtcacccg
    12181 cgtttcccgc ggtcgtaaga ttaaagaagt tatctgggga ctgatcctcg gcagcaccgt
    12241 cggttgctgg ttcttcttcg gcgtaatgga aagctatgcc attcatcagt ttatcaatgg
    12301 cgtaatcaac gtcccacagg tgctggaaac actgggcggc gaaacagctg tacagcaagt
    12361 tctgatgtcg ttgccagccg gtaaattgtt cctcgccgca tacctgggcg tgatgattat
    12421 tttccttgcc tcgcatatgg atgcagtggc ctacaccatg gcggcgacca gtacgcgtaa
    12481 tctccaggaa ggtgacgatc ctgaccgtgg gctgcgtctt ttctggtgcg tggtgatcac
    12541 tctgatcccg ctttccatct tgtttaccgg tgcttcgctg gaaacgatga aaaccaccgt
    12601 cgtgctcaca gcccttccct tcctcgtcat tttactggtg aaagtcggcg gatttattcg
    12661 ctggctgaaa caggattatg ccgacattcc ggctcatcaa gttgaacatt atctcccgca
    12721 gacaccggtt gaagccctgg aaaaaacacc agtgctccct gcgggaaccg tattcaaagg
    12781 cgacaactga gcgccatcat cctaacgata aaggtatccc tatgagcaat ctgagccctg
    12841 actttgtact acccgaaaat ttttgcgcta acccgcaaga ggcgtggacc attcctgccc
    12901 gtttttatac cgatcagaac gcgtttgaac acgaaaaaga gaacgtcttc gccaaaagct
    12961 ggatttgcgt cgctcacagc agcgaactgg cgaatgccaa tgattatgtg acgcgtgaga
    13021 tcattggcga aagcatcgtg ctggtacgcg gtcgtgataa ggttttgcgc gcgttctata
    13081 acgtgtgtcc gcaccgtggt catcagttgt tgagcggtga aggaaaagca aaaaatgtga
    13141 ttacctgccc atatcacgca tgggcattca aactcgatgg caatctggcc catgcacgta
    13201 actgcgaaaa cgtcgccaat ttcgatagcg acaaagcgca actggttccg gtgcgtctgg
    13261 aagaatatgc cggattcgtc ttcatcaaca tggaccccaa cgccaccagc gtagaagatc
    13321 agttacccgg tctgggcgcg aaagtgctgg aagcctgccc ggaagtccac gatctgaaac
    13381 tggcggcccg ctttaccacc cgcacgcctg ccaactggaa gaacattgtc gataactatc
    13441 tcgagtgcta tcactgtggt ccggcgcatc caggtttctc cgactccgta caggttgatc
    13501 gctactggca caccatgcac ggtaactgga cgctgcaata cggtttcgcc aaaccgtccg
    13561 aacagtcgtt taaatttgaa gagggtacgg atgcggcatt ccacggtttc tggctgtggc
    13621 cgtgcacgat gctgaacgtc accccgatca aagggatgat gacggtcatt tatgaattcc
    13681 cggtggattc tgaaactacc ctgcaaaact acgatattta cttcaccaat gaagagttaa
    13741 ccgacgagca aaaatcgctg attgagtggt atcgcgatgt gttccgtccg gaagatttac
    13801 gtctggttga aagcgtacag aaagggctga aatcgcgtgg ctatcgtggt caggggcgca
    13861 tcatggccga cagtagcggt agtggcattt ccgaacatgg tatcgcccat ttccataatc
    13921 tgctggcgca ggtgtttaag gactaatgac atcggcggcg gtattttccg ccgctgggct
    13981 gatttttgat ggagtacagc aatgtcagac tatcaaatgt ttgaagtaca ggtgagtcag
    14041 gtggaaccgc ttaccgaaca ggtgaaacgc ttcacgctgg tggcaaccga tggcaaaccg
    14101 ctgcctgcgt tcaccggagg aagtcacatc attgtgcaga tgagcgatgg tgataaccag
    14161 tacagcaatg cgtattcact actgagttcg ccgcatgaca cctcttgtta tcagattgcc
    14221 gttcggctgg aggaaaactc gcgcggcggt tcccgctttt tgcatcagca ggtaaaagtg
    14281 ggcgatcggt taacgatttc aacgcctaat aacctgtttg cgctaattcc ctcagccaga
    14341 aagcatctgt ttatcgcggg cggtattagt atcacccctt tcctgtcgca catggcagag
    14401 ttgcaacaca gcgacgtcga ctggcagcta cattactgct cgcgaaatcc agaaagttgc
    14461 gcatttcgtg atgagctagt ccagcatccg caggctgaga aagtccattt gcatcattca
    14521 tcaaccggaa cacgactgga attagcgcga ttattggcgg atatcgaacc tggcacacac
    14581 gtttatacct gtggccccga ggcgctaaat gaagcggtaa gaagtgaagc tgcgcgtctg
    14641 gacatcgccg ccgatacgct gcactttgag caatttgcta tcgaagacaa aaccggcgat
    14701 gcctttaccc tggtgcttgc ccgttccgga aaagagtttg tggtgccgga agagatgact
    14761 attttgcagg ttattgaaaa taataaagcc gcgaaagtgg aatgtttatg tcgtgaaggg
    14821 gtatgcggaa cctgcgaaac agcaatactg gaaggtgaag ctgaccatcg ggatcagtat
    14881 tttagcgatg aagagcgtgc cagccagcaa agtatgttga tctgttgttc gcgtgcgaag
    14941 ggtaaacgcc tggtgttgga tttgtagttt gccaaagccg gatgtggcgc tgagcgcgcc
    15001 cagtccggct tcggaagatt ttactgcgga tattcctgca ataaattgtg taatgcttcc
    15061 gccatcagct caccacgcca gccggaaatc agctccggca aattgttctg cggtttcagt
    15121 ttccagtgcc agttcagcag ttggttgatt tgccgacgcg atgccagcaa ttcggcgctg
    15181 atcttatgcg tttcgctcac gtcagtaatc agcgacttaa tcgctttaaa cgctttacga
    15241 taacccggca tgtccatcag gttaagcatc ggctgcggta aggcctcttc cggcaatgtc
    15301 tgcgcttttt ccaccagcgc cagcagcgtt ttaccgtgaa agcggatttc gctaccggat
    15361 aaacccaggc tgtccagttc gcctaaactt cccggcatat aacgcgcgac cgaccacaaa
    15421 tgctcttcac gcacgacaaa gttcaccgcc agatcacgct cacgagcctt gcgcagtcgc
    15481 cagtcggcta acagttgcag acaggccagt tggcgtgtgc gtaattgcca ggcattggtg
    15541 atatcacgcc aggcatcttc cggcgcaacg acttcctgac gacgcatttg catcaggcgg
    15601 cattcatcca gcgccgcagg tagccagccg gaggcctccg tttctaccat aagcttggcg
    15661 gtgatcggta acagatacca gacatccgcc gctgcgtatt cacactgacg ttcggtcagc
    15721 ggtctggcca gccagtcggt gcgcgattca ctcttgtcca gcgtaacgcc ggaatactct
    15781 tccaccatgg aagcgaaacc ccatgacatc gggcgtccgc agaaggcagc aaggatttgc
    15841 gtgtcaatca ggggttgtgg taattcgcca aagacattga ggaacacttc cagatcttca
    15901 ctgcctgcat ggagaaattt tgtgatggac ggatcgcgca ggatcgcttt cagcggtgac
    15961 cagtcggtga tcccgagagg atcaattagc gccagatgct caccatcgaa aagttgaatc
    16021 aaccccagct gcgggtaata agtgcgcgta cgaacaaatt cagtatcctg ggctatcgcc
    16081 ggaaagacac ggacggcttc acacaaagaa gccagcgcat cgtccgtggt aatcatttgg
    16141 taattcaaat tgtattctct ttagtgggcg tcaaaaaaaa cgccggatta accggcgtct
    16201 gacgactgac ttaacgctca ggctttattg tccactttgc cgcgcgcttc gtcacgtaat
    16261 tctcgtcgca aaatttttcc gacgttagat ttcggtaact catcacgaaa ctccaccagc
    16321 ttcggtactt tgtagcccgt gagctgacgg cagcaaaagg tcaccagtga ctcttcggta
    16381 agcgatggat cttttttcac tacgaagatt ttcaccgctt caccactgga gccggaaggt
    16441 acgccaacag ccgcgacttc ctgtacgcca ggatgctgca tgacgacatc ttcaatctcg
    16501 ttgggataga cgttaaaacc ggaaaccaga atcatgtctt ttttacgatc gacaatgcgc
    16561 aggaaccctt cttcatccat caccgcgatg tcgccggtgt gtaaccagcc atttttgatg
    16621 atctcatcgg tagcatcggg acgctgccag taacccagca tcacctgcgg tcctttgaca
    16681 caaagctcac ccggttgacc cggtggtact tcattatcgt cgtcatccac cagtttggct
    16741 tccgtcgacg gcaccggcaa accgatgcta ccactatgat aatcaatatc atatgggtta
    16801 acgctgacca gcggcgcaca ctcggtaaga ccatagcctt ccagcagata ctgtcctgtc
    16861 agtttcaccc aacgctctgc caccacttgc tgcactggca tcccaccgcc tgcggaaaga
    16921 tgcagactgg agaaatccag ctgctggaac tctttattgt tcagcaacgc attgaacaag
    16981 gtgttaacgc cagtgatagc ggtaaacgga tatttcgcta actcttttac caaccctgga
    17041 atatcgcgcg ggttagtgat aagcaggttc tgcccaccca gttcgataaa cagcaggcag
    17101 ttaatggtca gggcaaaaat gtgatacagc ggcagtgccg ttactaccag ttctttacct
    17161 ggatgcagca acggcccata ggtggcgttg acctgttcca ggttcgccag catattgcgg
    17221 tgagtcagca tcgcgccttt cgccacacct gtggtgccgc cggtgtattg caggaaggct
    17281 aaatcttccg gcaccagttc gggtttgacg tactgcatcc ggtagccgtt atgcagtgcg
    17341 ctacgaaatg aaatggcatc tggcagatgg tatttcggca ccaaacgctt gatgtattta
    17401 acaacgaaat tgactaccgt gccttttgcc gtagatagct gatcgcccat acgggtcaga
    17461 attacgtgct gaacggcggt tttatcaacc actttttcca gtgtgtgagc aaagttagac
    17521 acgataacaa tcgccgatgc gccgctatcg ttaagctgat gctcaagctc acgcggggta
    17581 tacaacgggt taacgtttac gacgatcatc ccggcacgca aaatgccaaa cagcgccacc
    17641 ggatattgca ataaattagg catcatcaac gcaacgcgat cgcctttctt cagccccaac
    17701 ccttgttgca aataagcggc aaacgcgcga ctgcgttctt ccagcttgcg gaaggtcatt
    17761 acctccccca tattcacaaa cgcaggctga tcggcgtagc ggacgaccga ctgctcaaac
    17821 atatctatca gagattgata acggtcaggg ttgatctccg tcggaacatc cgcgggataa
    17881 cggttaagcc aaaccttctt caattcttca cctctaaaat gcgtgttcgt cgtcatcgca
    17941 accccaagtg ataaacatgt tgttaacata acattaactc atcttaccag cttgataatt
    18001 agacaacgaa aaggttgcga agcgcgtcac tatttatttt tatagttgcc gtatgaacgc
    18061 agaaacagcg gaccagccgc tgtttctttt tctttaaaaa caacttgtta ctcggttaca
    18121 attgtttcaa ctcttgcagg gccaggatta taccaacccc atccgccgta accatagggc
    18181 cagccgcgtc caccataaaa ccatggatca attggctgcg gcggcataat cacctgttga
    18241 gtgatatgcc agcgtttgta acctgttacc tgcataacca taaatttata tggtgcatta
    18301 tttactttgc catccactgt gccagtgatt ggcccgacaa ccgtcaccag ttgtccacga
    18361 aaatcgaccg gatcaagaaa accgttcaca tcggcataaa tgcgcccgcg tgaaggttct
    18421 cccaaggttg gtcttgctcc gctgtccagc ggtacggtag caatttccag gcgggttttc
    18481 ccttgctggt tttgtaccac aaccactttg ccgccaaagc gcgcctcctg accaacatat
    18541 aaatctggcg cattcatcac gcgtaccaga tcttgctgtg gtgttggact gctgccttta
    18601 atggcgtccg gcacagtgac acaaccgctc agcattagcg caaacgtacc tgccagtatg
    18661 cctttgataa cattcttttg aaccgccatg atgcgactcc tttttttctc agggcatact
    18721 cttaagattc attctttgcc cggaagtttc ttccatgcga cgttgttacg taaataaacc
    18781 ggttcggcat gttccaccgc caccgtttta ccctcggcaa acatctgaca cgcaatcggc
    18841 agcatatctt ccgccgctgg cagtaaaact tctccgtcgc gcaaaaccag cccgctctct
    18901 ttacggagat ccggccaggc ttgccagccc gtccctaccg ttacccattc gccggaaagc
    18961 tgttgcattc gttcatgaac gagttcgggt ttgagtacgg cttcggtttc ttcaccgcgc
    19021 cagataccgt tttcatcacg ctgatattcg gcccagtaaa cttcgcccat tcgcgcgtca
    19081 atggctgcca gcacgcgggt tgcgccgttt ttgcgccacg ccccctgcgc catcgtcatt
    19141 agcgtagaga caccaatcat cggtaactcc gcgccaagcg ccagcccttg cgcaatacca
    19201 ataccaatgc gcacgccagt aaagctaccg gggccgcgcc cgtaagccag agcgtttatg
    19261 tcagtcaggg aagttccgct ggtggtcagg atatcctgca ccatcggtaa gattcgttga
    19321 gtatgttcac gagggcaaag ctcaaaatga gcgttgacat taccgtcgtt ccacagagcg
    19381 acagagcaga cctctgtcgc ggtatcgata gccagaattc gcatgggtct tcgtgcttag
    19441 atcaataaaa aggcgcgcat cataccatac tccgtaacaa attacctgga ggatggtatc
    19501 gcacaaggaa acgaaccgca cgggcaatgt cacgggtgcg tggcgcgggc ggcagactgg
    19561 cgagaaacgt cgcgccgtaa ggacgcatca ccagccgatt gtcacaaatt accagcacgc
    19621 cgcgatcgtc ggcgtcgcga atcagtcgcc ctactccctg cttgagagta atgaccgcat
    19681 ccggcagctg cacttcatcg aacgggtcgc caccgcgcaa acgacaatct tccatgcgcg
    19741 cttttaacag tagatcatcc ggcgaggtaa acggcaattt gtcgataatt accaatgaca
    19801 atgtatcgcc acgcacgtcc accccttccc agaaactact ggttgccaca agaagcgcgt
    19861 taccggcgct gacaaattgc tgcaacagtt gccctttgct ggtttccccc tgcaacaaaa
    19921 cgggaagcgt catggtagcg cggaactgct cggccagatc gcgcatcatg gcatgcgagg
    19981 tacaaagcat aaaacaacga ccgttgttag cttcgatgat cggtcgcagc attgccgcca
    20041 gttgccgtgc acaacccggt tgattggttt gcggcaggtt gcgcggcaca cagagtaacg
    20101 cctggcggct gtaatcaaac gggcttggca atagcaacga ctccgcctgt tctatcccaa
    20161 gccgcgaggt gaaatgatgc agatcgtcgt tcaccgacag cgttgctgag gtgaagatcc
    20221 agctaccggg cttttgcgcc attaactctt tgaatttatc cgccacgctg agcggcgtga
    20281 gagccagagt aaaatggcgc gaagtgcatt cgtaccagta gctgtagccc ggctgattga
    20341 tctcttttag ccgcttcagc cgtgtgcgat acaacgtggc gcgctcaaat gccgcatcca
    20401 gcaaggcgga acgccccagt gacagtttcg ccacgtcata acaaagttcc agggtgtcat
    20461 cgagcagtaa aaatgcccgc tgaatttgcg gattagctaa cagctcacgc aggttaccgc
    20521 gataacctgg ctcaccgagt tgcagacgaa aatcctgtgc actctgggca agacgatcag
    20581 cgcacttttg taactgctgg gtgtctttta attcggtgcg gtaggcgatg gtgatgtctt
    20641 ttgccaggtc gagcagttgt cgactggaga gtgactgacc aaaatactgg ctggcaatgt
    20701 ccggtagctg gtgggcttcg tcgaagatca tgacgtccgc ttcagggatc aactcgccaa
    20761 atccactctc tttaaccacc atatccgcca gaaagagatg atggtttacc accaccacat
    20821 cggcgtccat cgctttttta cgtgctttga ccacaaagca atctttatac atcgggcagt
    20881 cgctgccaag acagttgtcg ttggtgctgg tgaccagcgg ccacgcctgt gaatcttccg
    20941 ccacgctgac gcaggtgctg atatcaccat cgactgtttg attagaccag gagcgcagca
    21001 ggatcacatc gcttaagatt tgtaccggca gatcgccccc cgccagcgcc tgctgttcga
    21061 gacgttcgag gcagaggtag tttgagcgcc ctttcagcag cgccacgttg cccgtatatt
    21121 tcaatgcctt tgagactgtt ggcaaatcgc ggctgtagag ctgatcctgc aacgcttttg
    21181 agccggtcga gataatgact ttctttttcg cccgcagcgc aggagccagg taagcgtagg
    21241 ttttgcccgt accggttcct gcttccacca ccagcggctg gcctttttct atcgcctggg
    21301 tgacggctac cgccatctgt cgctgtggtt ctcgcggctt aaagcctggt atcgctttcg
    21361 ccagctgacc gtctggtgca aaatcgtccg tcacactacc ccctgttgat ttgaacaggg
    21421 attatgtcag gatgagggcg ctttcgccag ttgaagtggt gacggcaacc tcacattgtg
    21481 gcagtctttg cagcacgaaa atggaagtat aatgaggaca aaatgatgac tatcgttcgt
    21541 atcgatgctg aagcccgctg gtctgatgta gtaatccaca acaacacgct ctactacact
    21601 ggtgtaccgg aaaacctcga cgccgatgcc tttgagcaaa ccgccaacac gctggcacag
    21661 attgacgccg tgctggaaaa acagggcagc aataaatcga gcattctgga tgccaccatt
    21721 ttcctggccg ataaaaacga cttcgcggcg atgaataaag cgtgggatgc ctgggttgtc
    21781 gcgggtcatg cgccagtgcg ctgcacggta caagcgggtt tgatgaaccc gaagtataaa
    21841 gttgaaatta agattgtggc tgcggtgtaa gctttatcga agcaaaataa gtcagacgat
    21901 aatttatcga taatactggt cggttttaca taaatcgacc agagaataag atattactgt
    21961 tgatgcttct gctaattcat tatttatatt tataatttca attttatcta taaaactaac
    22021 tatataaata aacataacca tgccgttgtg ttcgcccttt gattagcaac tctggttttc
    22081 tttcttagct gaacaatctg gttatagcgt gcggactgac cgttaatttt ctccttctta
    22141 ctggataaca caccatgagc tgctaccttt aaaacacctg aatgtcaaca ggttaactcg
    22201 tgccatatcg ttataatccc tttgatatta gatgcaaatt a
//
LOCUS       DAZPDU010000059        21955 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZPDU010000059 DAZPDU010000000
VERSION     DAZPDU010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21955
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.059"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..989)
                     /gene="cmtA"
                     /locus_tag="ACHURS_002041"
     CDS             complement(<1..989)
                     /gene="cmtA"
                     /locus_tag="ACHURS_002041"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFR1237781.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     LVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWVFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVATLVSFA"
     gene            complement(1017..1460)
                     /gene="cmtB"
                     /locus_tag="ACHURS_002042"
     CDS             complement(1017..1460)
                     /gene="cmtB"
                     /locus_tag="ACHURS_002042"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFR1237782.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTAASEKQLADIISRG"
     gene            complement(1774..3765)
                     /gene="tkt"
                     /locus_tag="ACHURS_002043"
     CDS             complement(1774..3765)
                     /gene="tkt"
                     /locus_tag="ACHURS_002043"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFR1237783.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            4043..4801
                     /gene="loiP"
                     /locus_tag="ACHURS_002044"
                     /gene_synonym="yggG"
     CDS             4043..4801
                     /gene="loiP"
                     /locus_tag="ACHURS_002044"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFR1237784.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            complement(4944..5864)
                     /gene="speB"
                     /locus_tag="ACHURS_002045"
     CDS             complement(4944..5864)
                     /gene="speB"
                     /locus_tag="ACHURS_002045"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFR1237785.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLTFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(6002..7978)
                     /gene="speA"
                     /locus_tag="ACHURS_002046"
     CDS             complement(6002..7978)
                     /gene="speA"
                     /locus_tag="ACHURS_002046"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFR1237786.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(7987..8118)
                     /gene="yqgB"
                     /locus_tag="ACHURS_002047"
     CDS             complement(7987..8118)
                     /gene="yqgB"
                     /locus_tag="ACHURS_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFR1237787.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            complement(8212..8355)
                     /gene="yqgG"
                     /locus_tag="ACHURS_002048"
     CDS             complement(8212..8355)
                     /gene="yqgG"
                     /locus_tag="ACHURS_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFR1237788.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(8466..8717)
                     /gene="yqgD"
                     /locus_tag="ACHURS_002049"
     CDS             complement(8466..8717)
                     /gene="yqgD"
                     /locus_tag="ACHURS_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFR1237789.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            8773..9927
                     /gene="metK"
                     /locus_tag="ACHURS_002050"
     CDS             8773..9927
                     /gene="metK"
                     /locus_tag="ACHURS_002050"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFR1237790.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            10363..11757
                     /gene="galP"
                     /locus_tag="ACHURS_002051"
     CDS             10363..11757
                     /gene="galP"
                     /locus_tag="ACHURS_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFR1237791.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            11834..12331
                     /locus_tag="ACHURS_002052"
     CDS             11834..12331
                     /locus_tag="ACHURS_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFR1237792.1"
                     /translation="MKTSRLPIAIQQAVMRGLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            12426..13133
                     /gene="endA"
                     /locus_tag="ACHURS_002053"
     CDS             12426..13133
                     /gene="endA"
                     /locus_tag="ACHURS_002053"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFR1237793.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            13213..13944
                     /gene="rsmE"
                     /locus_tag="ACHURS_002054"
     CDS             13213..13944
                     /gene="rsmE"
                     /locus_tag="ACHURS_002054"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFR1237794.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            13957..14907
                     /gene="gshB"
                     /locus_tag="ACHURS_002055"
     CDS             13957..14907
                     /gene="gshB"
                     /locus_tag="ACHURS_002055"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFR1237795.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            15016..15579
                     /locus_tag="ACHURS_002056"
     CDS             15016..15579
                     /locus_tag="ACHURS_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFR1237796.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            15579..15995
                     /gene="ruvX"
                     /locus_tag="ACHURS_002057"
     CDS             15579..15995
                     /gene="ruvX"
                     /locus_tag="ACHURS_002057"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFR1237797.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(16170..17150)
                     /locus_tag="ACHURS_002058"
     CDS             complement(16170..17150)
                     /locus_tag="ACHURS_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFR1237798.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFEAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSSKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQKREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSFQQRVGEGRL"
     gene            17168..17872
                     /gene="yggS"
                     /locus_tag="ACHURS_002059"
     CDS             17168..17872
                     /gene="yggS"
                     /locus_tag="ACHURS_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFR1237799.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            17890..18456
                     /gene="yggT"
                     /locus_tag="ACHURS_002060"
     CDS             17890..18456
                     /gene="yggT"
                     /locus_tag="ACHURS_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFR1237800.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            18453..18743
                     /gene="yggU"
                     /locus_tag="ACHURS_002061"
     CDS             18453..18743
                     /gene="yggU"
                     /locus_tag="ACHURS_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFR1237801.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQGNSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            18751..19344
                     /locus_tag="ACHURS_002062"
     CDS             18751..19344
                     /locus_tag="ACHURS_002062"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFR1237802.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARYAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            19337..20473
                     /gene="hemW"
                     /locus_tag="ACHURS_002063"
     CDS             19337..20473
                     /gene="hemW"
                     /locus_tag="ACHURS_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFR1237803.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDNALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            20740..21726
                     /locus_tag="ACHURS_002064"
     CDS             20740..21726
                     /locus_tag="ACHURS_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFR1237804.1"
                     /translation="MKMRSFTRSLVCASLLALVSTGVNAAEKVTLKLAHNLERSHVVH
                     QSFEELAKEVKQLSEGNMVIRIYPSSQMGNARETMELLQNGALDMTKGSASDLESFDN
                     IYAIYNLPFLFKDQAHFNKVVFGEVGKEIMDSTKDKGFFALSAYVAGTRSFYAKKPIT
                     KPEDLKGLKIRVQPSPTTIKMIELMGGSPTPISFGEVYTAMQQGVVDGAENNVPSWVQ
                     TRHIEIAKVFSEDEHASIPDFLVISTKTWNKLTPEQKQILETAAKKSEAYQQKLWEKI
                     DADTRAQAKAMGGEIVKVDKAPFRAAVQPLFDDFKKDPKQAALLEKFDNAAQ"
     gene            21771..>21955
                     /locus_tag="ACHURS_002065"
     CDS             21771..>21955
                     /locus_tag="ACHURS_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFR1237805.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGA"
BASE COUNT         5426 a         5709 c         5590 g         5230 t
ORIGIN      
        1 atagcaaagg acaccagggt agctaccgta acaccggcaa ttgtcgccag gaacgagcct
       61 ttcggcgtca gtgccaggta agcaaagata gaccccggac ttgggccagc caccagacca
      121 ccgtccagta agttaaacac ccaggtgcca gacataccgc ccgcaatcat ggcaataatg
      181 gtcagcggct tcatcagcac atacgggaaa tacagttcgt ggatcccacc gaggaagtga
      241 ataatcatcg ccccgggcgc agaacgttta ctcatccctt taccaaacaa ggtaaacgcc
      301 agcagcagcc ctaagccagg cccaggattg gacgccacca taaagaagat ggatttaccg
      361 ttaaccgaag cctgttgcat tcccagcgga taatagacgc cctgatcgat cgcattattg
      421 aggaaaagca ctttcgccgg ttcattgatg actgacagca gaggcagata acccgcatgt
      481 accagcgcct caatacactc tttgacgaga gtattggcaa ttaacaccgc cgggccgata
      541 acttcaaaac ccagcagaca aaggagcatc cccgcgatac ctaatgagaa gttattgata
      601 accatctcaa aaccggcagg tatgcgtttt tccagtgcct tatcaacata ttttatgacc
      661 agcccaccga gcggccccat aatcattgag ccaaggaaca tcgggatctc tgcgccgacg
      721 atcacaccaa tagtacctat tccgcccatg accgcgccgc gtttaccgcc gaccagatga
      781 ccaccggtag aaccaatcat cacgggcaat aaataggtaa tcatcgggcc gacaattttg
      841 gcgaaatgtt cgttaggcag ccaaccggtg ggaataaata acgcagtaat aaaaccccag
      901 gcaataaaag caccaatatt ggggatgacc attgccgtca aaaagccccc aaaagcctgg
      961 acctttgcac gagcagactt gttttccata atattatcct gtagaggaga gagtaattat
     1021 ccgcggctga ttatgtccgc taattgtttt tctgatgctg ctgttaagag ttgttcgagt
     1081 atttcttctt cgcacagtaa ttcacttaac gcctgaatag cgccaatgtg cgaatcggca
     1141 tcggcagcag aaaggccgat gagtaatttt attggttcgt cattccccgg aaaataaaca
     1201 ccttgttcaa gtaatgtcaa agacatcccg gttttaagcg ccccacattc cggtcgcgca
     1261 tgaggcattg ccacgcctgg tgcgagaata taataagggc cattgttaat ggtggaatct
     1321 ttaattgcct gaatgtaatt ctcgctgata tagtttttat ccagcaatga taccatcgag
     1381 aaatcgatag cttcctgcca atcttttgct gaatgtataa ctgagattga tgattctgga
     1441 aaatagtcac taagccgcat aaattatcct tattttattt ttagggtacc gtccggcggt
     1501 aaaaataaaa tactcgtgcg gtttaatgaa agcaattgag cagggaaacc ggtgttttga
     1561 aaacagtgaa ttattaattt cttatataac attgagttat agatataaca acaaatgtca
     1621 atacgcatat cgtgatgcat attacataat tgatatttat tgcatataaa tatttgtgat
     1681 ctacaacacg ctttatctat tgcttgtccg caaacggaca tatcaaggta ataaaaaagg
     1741 tcgccgaagc gaccttttta cccgaaatgc taattacagc agttcttttg ctttcgcaac
     1801 gacgttgtcg acggtgaagc cgaactcttc gaacagcaac tctgccggag ctgactcacc
     1861 gaaggtggtc ataccgatga tagcgccatt caggccaaca tacttgtacc agtagtcagc
     1921 aatacccgct tctaccgcaa cgcgtgcagt aaccgctttc ggcagtacgg attcacggta
     1981 agccgcatcc tgcttgtcga atgcatcggt agacggcatg gaaaccacgc gcgccttcac
     2041 gccttcggca gtcagttttt cgtaggcggc aacagccagt tcaacttccg aaccggtcgc
     2101 gatgaagatc agttccggct gaccagcaca gtctttcagc acataaccac cgcgcgcgat
     2161 gtttgccagt tgctcttcag tacgttcctg ctgcgccaga ttctgacggg agaggataag
     2221 cgcggtcgga ccgtcctgac gctcaacacc gtatttccac gcgaccgcgg attcaacctg
     2281 gtcacacgga cgccatgtag acatgttcgg ggtcacgcgc agagaagcga cctgctcaac
     2341 cggctggtga gtcgggccgt cttcgcccag accgatggag tcgtgggtgt aaaccatcac
     2401 ctgacgctgt ttcatcagcg cagccatacg tacagcgtta cgtgcgtatt cgacaaacat
     2461 caggaaggtg gaggtgtacg gcaggaagcc accgtgcagg gagataccgt tagcaatcgc
     2521 ggtcataccg aactcgcgaa caccgtagtg gatgtagtta cccgcagcat cttcgttgat
     2581 tgctttagaa ccagaccaca gggtcaggtt agacggtgcc aggtcagcgg agccgccgag
     2641 gaattccggc aacagcgggc cgaacgcttc aatagcattc tgagacgctt tacggctggc
     2701 gattttcgcc ggattagcct gcagtttagc gataaactct ttcgctttgg cgtcgaagtc
     2761 agacggcatt tcgcctttca tacggcgggt aaattcagcc gcttcctgcg gataagcttt
     2821 cgcgtaagcc gcaaacttct cattccatgc agattctttc gcctggcctg cttctttcgc
     2881 atcccactgc gcatagattt cagacgggat ttcgaacggt gcgtatttcc agcccagctg
     2941 ttcgcgggtc agagcgattt ctgcatcacc cagcggcgca ccgtgggagt cgtgagtacc
     3001 ggctttgttc ggggaaccga aaccgatgat ggttttgcac atcagcaggg acggtttgtc
     3061 agtcactgcg cgcgcttctt ctactgcgcg tttgatggat gctgcgtcat gaccgtcgat
     3121 gtcgcgaata acgtgccagc cgtaagcttc gaaacgcatt gcggtgtcgt cggtgaacca
     3181 gccttcaacg tgaccatcaa tagagatacc gttgtcatcg tagaacgcaa tcagtttacc
     3241 cagcttcagc gtacccgcca gagagcaaac ttcgtgggag atgccttcca tcatgcagcc
     3301 gtcgcccatg aaggcgtagg tgtagtggtc gacaatgtcg tggcccggac ggttaaactg
     3361 cgccgccagc gttttttctg caatcgccat accgactgcg ttggcaatac cctgacccag
     3421 cggaccggtg gtggtttcca caccagcggt ataacctact tccgggtggc ccggagtttt
     3481 agagtgcagc tgacggaagt ttttcagttc ttccatcggc agatcgtaac cggtgaggtg
     3541 cagcaggctg tagatcagca tggagccgtg gccgttggac agcacgaagc ggtcacggtc
     3601 agcccaggac ggattctgcg ggttgtgttt caggaaatca cgccacagga cttcggcaat
     3661 gtcagccata cccatagggg cacccgggtg accggatttg gctttctgta ctgcgtccat
     3721 gctcagcgca cgaatagcat tggcaagctc tttacgtgag gacattttga ctccagatcg
     3781 gatgatgaag ggcacgccct taacgacttg acgacagcgc gttttgggct acgccggaaa
     3841 atttgccaac aatttaccgc aagccgcgcg tcatgtacat ggaacatcct tttgccgctt
     3901 cagaaatctc tggatcatgc tcgcatgttg cgcaatctac tcgcccgtcc gctgcgcttt
     3961 tccttatact gagactgagc gtcgattcac ctgcaaacgg cgcattttta gaataatcct
     4021 gaccttgtgc ggaagagaaa acatgaaaat tcgcgcctta ttggtagcaa tgagcgtggc
     4081 aacggtactg actggttgcc agaatatgga ctccaacgga ctgctctcat caggagcgga
     4141 agcttttcag gcttacagtt tgagtgatgc gcaggtgaaa accctgagcg atcaggcatg
     4201 tcaggagatg gacagcaagg cgacgattgc gccagccaat agcgaatacg ctaaacgtct
     4261 gacaactatt gccaatgcgc taggcaacaa tatcaacggt cagccggtga attacaaagt
     4321 gtatatggcg aaggatgtga acgcctttgc aatggctaac ggctgtatcc gcgtctatag
     4381 cgggctgatg gatatgatga cagataacga agtcgaagcg gtgatcggtc acgagatggg
     4441 gcacgtggcg ttaggccatg tgaagaaagg gatgcaggtg gcgcttggta caaatgccgt
     4501 gcgagtagct gcggcctctg cgggcgggat tgtcggcagt ttatctcaat cacaacttgg
     4561 tgatctgggc gagaaattag tcaattcgca attctcccag cgccaggaag cagaagccga
     4621 tgattattct tacgatcttc tgcgccaacg cggcatcagc ccggcaggtc ttgccaccag
     4681 ctttgaaaaa ctggcgaaac tggaagaagg ccgccaaagc tcaatgtttg acgaccatcc
     4741 ggcatccgcc gaacgcgccc agcatattcg cgatcgcatg agcgcggatg ggattaagta
     4801 aagcctggtg gtgtcgaacg taggtcagat aaggcgttca cgccgcatct gacatcaatg
     4861 gcacatttgt taccctgtgc gcattgccgg atgcgatgct gactgcatct tatccggcct
     4921 acgggtggca tcgcatctgg tgcttactcg ccctttttcg ccgcttggat atacagcatc
     4981 tccagcgcca gcgtcgccgc agccagagcg gtgatttccg actgatcgta tgccggagcc
     5041 acttccacta cgtccatccc aacaatgttg agatctttca ggccgcgtac cagtttaata
     5101 gcgcgatcgg aggtcaggcc gccaatcact ggcgtaccgg tgcctggtgc aaaagcagga
     5161 tccaggcagt cgatatcaaa agtcaggtaa accggcatat cacccacaat ctgtttcact
     5221 tgggcgataa cgtcatccac gctgcgatcg ttcacctggc aggcgtccag cacggtaaag
     5281 ccgttgtctt tatcaaactc ggtacgaata ccaatctgca cggaatgatt cgggtcgatc
     5341 agaccttctt tcggcgcggt atagaacata gtgccgtggt caaattcaca accgttcgca
     5401 taggtatcgg tgtgggcgtc aaagtgtacc agcgccattt tgccgaaatg cttcgcatga
     5461 gcacgcagca gcggcagcgt aacaaagtgg tcaccaccga aggtgagcat acgcttaccg
     5521 gcagccagca gcttctcggc gtgcgcctgc agcttttcgc tcatctcacg ggcatcgcca
     5581 aaggcatata ccagatcgcc gcagtccacg acgttcagac gctcacgcat gtcgaaattc
     5641 cacgggaagc ggttgtgttc ccaggccaga ttcgtcgaaa cctgacggat cgctgccgga
     5701 ccgtggcgac cacccgcacg accagaagtg gccatatcga acggcacgcc agtaatcacc
     5761 cagtctgcat cgctgtcata cggctggaag ttcatcggca ggcgtaaaaa accaaaggca
     5821 ttggaaacca gtgagttatc gtattgatga cctaaggtgc tcatggatgg actcctatta
     5881 caaagtcgat ataaaaaaac ccttccgcgt cgttaggccc gacgaggaag ggttggattt
     5941 gtcacaataa attgtggcgg attatcaccg ctaaattaag cggattcaag taacacagga
     6001 cttactcatc ttcaagataa gtataaccgt acaaacctgc ctcgaactct tcaaggaact
     6061 gttgttgcag ttcagcatca agatcggttt tcttcacttg atcgcggaac tgggttaaca
     6121 gcgttttcgg atcgagctgt acatattgca gcatgtccgc cacggtatcg ccttcgtcag
     6181 acagttctac ttctacgcta ccgtcaggga acacgaacac gtcaaccgct tcggtatcac
     6241 cgaacaggtt gtgcatattg ccaaggatct cctgatatgc gccgaccata aagaaaccga
     6301 gcatcggcgg attctctgga tcgtactccg gcattggcat tgtcgtggca ataccatcac
     6361 catcaatata gtggtcgata gcaccgtcag agtcacaggt aatatccagc agcacagcgc
     6421 gacgttccgg cacttgatcc agcccttcca gcggcagaac cgggaacaac tggtcgatcc
     6481 cccatgcgtc cggcattgac tggaacagcg agaaattgac gtacattttg tccgccatac
     6541 gttcctgcag ttcgtcgatt atcggacgat gcgcacggtt ttgcggatcc agttgcttct
     6601 gcacttcatg gcacatgctc agatagagtt gttctgccca tgcacgttct tgcaggctaa
     6661 aggtgccgga agagtagccg atatgaatgt cgtgcagatc catctggctg tcgtgtaacc
     6721 attcacgcaa agaacggcga gttcccggtt cgtgcatttc ctgccaggtt tcccacatgc
     6781 tttgcagcgc gcgcggcgca tcttctgcgg gcgcggtcgg caccgtatat tcgttacgtt
     6841 ccacgccgat gatattcgac accagcacgg tatggtgcgc agtcaccgca cggcccgatt
     6901 cggtgattac cgtcggatgc ggcagaccat tttcttcaca cgcatcgcca atcgcccaga
     6961 tgatgttgtt ggcgtattca ttgaggccat agtttacgga gcagtcagac tgcgaacggg
     7021 tgccttcata gtccacgccc agaccgccgc cgacgtcgaa gcactgaata ttgacgccca
     7081 gcttgtgcag ttccacatag aaacgcgcgg attcacgaac gcccgtggcg atatcgcgaa
     7141 tattcgccat ctgcgaaccg aggtggaagt gcagtagttg caggctgtcg agacgcccgg
     7201 cttcacgcag ggtttcaacc agttgcagta cctgagtcgc agccaggccg aacttcgatt
     7261 tttccccgcc ggaggactgc catttacccg agccctgcga agccagacgt gcacgcacgc
     7321 ccagacgagg aacgacattc agacgttctg cttcatccag cacaatggcg atttctgaca
     7381 tcttctcaat gaccagatag accttgtgcc ccatcttctc gccaattaat gccaggcgga
     7441 tatattcgcg gtctttataa ccgttgcaga cgatgacgct acgggtcatg ccagcatgtg
     7501 ccagtactgc catcaactcg gctttggaac cggcttccag acccagcggt tcgcccgaat
     7561 gaatcaggga ctcaatcacg cggcggtgct ggttaacttt gatcggataa acaaggaagt
     7621 aatcgccgtt atagccgtag gattccctcg cacgtttgaa cgcggcgtta atggaacgca
     7681 aacggtgctg caggatctgt gggaaacaga acagtgcagg cagacgctga ccctgtgctt
     7741 cacgagtttt cactaactgc gcgagatcga cgcgagcttc cgggacgtcc gggtccgggc
     7801 acacgctgat gtggcccagc tcgttaacgt catagtagtt attgccccac caggcaatat
     7861 tgtaagtacg cagcatcttg ctggcttcct gggagctcat tgcaacctcc tgcatggagc
     7921 gtagtacacc gtgttcgccc gctgacgaag gcaaacccat agacatgtcg tcagacatag
     7981 cgaacctcaa attattttat taagtgtaaa acagttaacg actatcgcag cctggaactg
     8041 cgataacaac ccataagcac atggattttc cagcagtgaa tgctgacgct ccaactgcgc
     8101 aaccggtttc tttttcataa cattattaag cacataaccg aacgtaagtg tgacagttcg
     8161 gcgaaaccac gagaaaactc ttgtttttac aagagcgccc ttgttcagtc ctcagtaact
     8221 gtaaccagct cttgaatcct gagaagcgcc gagatgggta taacatcggc aggtatgcaa
     8281 agcagagatg cagagtgcgg ggaacgaatc ttcaccagaa cggtgagaca ggttaagcag
     8341 cagacaacgg ttcattattt cgtatcacct ccacggccgc ctgttaagac gaacccacaa
     8401 gccaaaactc tgatttcaac ccggctggaa gtggcaacac gaaagaaacg tcgtgtgctt
     8461 tttatttaag ccgcgcgccg cgttttatac cccacaatgg cagaaaattg caaaagataa
     8521 atacgcagaa tgccggcatt gtcaggaaaa atttccagcc acgtttttaa cagaatgaga
     8581 cacgattcaa aaaaaagtgg aaatcgggtg aagaattgac ctaaaatagc catccagatg
     8641 ttaatccatc cataccgatt aacactcaga ctgccagtgt ttttaacctg cagagtcgtg
     8701 gtaggatccg ctaccacaga aaatccacac aacagtttga gctaaccaaa ttctctttag
     8761 gtgatattaa atatggcaaa acaccttttt acgtccgagt ccgtctctga agggcatcct
     8821 gacaaaattg ctgaccaaat ctctgatgcc gttttagacg cgatcctcga acaggatccg
     8881 aaagcacgcg ttgcttgcga aacctacgta aaaaccggca tggttttagt tggcggcgaa
     8941 atcaccacca gcgcctgggt agacatcgaa gagatcaccc gtaacaccgt tcgcgaaatt
     9001 ggctatgtgc attccgacat gggctttgac gctaactcct gtgcggttct gagcgctatc
     9061 ggcaaacagt ctcctgacat caaccagggc gttgaccgtg ccgatccgct ggaacagggc
     9121 gcgggtgacc agggtctgat gtttggctac gcaactaatg aaaccgacgt gctgatgcca
     9181 gcacctatca cctatgcaca ccgtctggta cagcgtcagg ctgaagtgcg taaaaacggc
     9241 actctgccgt ggctgcgccc ggacgcgaaa agccaggtga ctttccagta tgacgacggc
     9301 aaaatcgttg gtatcgatgc tgtcgtgctt tccactcagc actctgaaga gatcgaccag
     9361 aaatcgctgc aagaagcggt aatggaagag atcatcaagc cgattctgcc cgctgaatgg
     9421 ctgacttctg ccaccaaatt cttcatcaac ccgaccggtc gttttgttat cggtggcccg
     9481 atgggtgact gcggtctgac tggtcgtaaa attatcgttg atacctacgg cggcatggca
     9541 cgtcacggtg gcggagcatt ctctggtaaa gatccatcaa aagtggaccg ttccgcagcc
     9601 tacgcagcac gttatgtcgc gaaaaacatc gttgctgctg gcctggccga tcgttgtgaa
     9661 attcaggttt cctacgcaat cggcgtggct gaaccgacct ccattatggt agaaactttc
     9721 ggtactgaga aagtgccttc tgaacaactg actctgctgg tacgtgagtt cttcgacctg
     9781 cgcccatacg gtctgattca gatgctggat ctgctgcacc cgatctacaa agaaaccgca
     9841 gcatacggtc actttggtcg tgaacatttc ccgtgggaaa aaaccgacaa agcgcagctg
     9901 ctgcgcgatg ctgccggtct gaagtaatct ttcttcacct gctttcaaag gccagcctcg
     9961 cgctggcctt tttcttttac ctcaccgcgg tggataggca ttcacgccgc atccggcaaa
    10021 aagctgcccg cacaataaca tcattcttcc tcaacacgtt ttatcgcaaa ttatcatcac
    10081 tactgaaacc gattacacca ataacaccag ccaaatattt gtaatatttt catattatta
    10141 ttcagttttc acagttgtta catttctttt cagtaaagtc ttaattgcag ataacagcgt
    10201 ttaatctatg atgatataac tcaattattt tcatgcactt aaaccataac taagataaat
    10261 gttagtgtaa gcgattacac tgatgtgatt tgcttcacat ctttttacgt cgtactcacc
    10321 tatcttaatt cacaataaaa aataaccata ttggagggca tcatgcctga cgctaaaaaa
    10381 caggggcggt caaacaaggc aatgacgttt ttcgtctgct tccttgccgc tctggcggga
    10441 ttactctttg gcctggatat cggtgtaatt gctggcgcac tgccgtttat tgcagatgaa
    10501 ttccagatta cttcgcacac gcaagaatgg gtcgtaagct ccatgatgtt cggtgcggca
    10561 gtcggtgcgg tgggcagcgg ctggctctcc tttaaactcg ggcgcaaaaa gagcctgatg
    10621 atcggcgcaa ttttgtttgt tgccggttcg ctgttctctg cggctgcgcc aaacgttgaa
    10681 gtactgattc tttcccgcgt tctactgggg ctggcggtgg gtgtggcctc ttataccgca
    10741 ccgctgtacc tctctgaaat tgcgccggaa aaaattcgtg gcagtatgat ctcgatgtat
    10801 cagttgatga tcactatcgg gatcctcggt gcttatcttt ctgataccgc cttcagctac
    10861 accggtgcat ggcgctggat gctgggtgtg attatcatcc cggcaatttt gctgctgatt
    10921 ggtgtcttct tcctgccaga cagcccacgt tggtttgccg ccaaacgccg ttttgttgat
    10981 gccgaacgcg tgctgctacg cctgcgtgac accagcgcgg aagcgaaacg cgaactggat
    11041 gaaatccgtg aaagtttgca ggttaaacag agtggctggg cgctgtttaa agagaacagc
    11101 aacttccgcc gcgcggtgtt ccttggcgta ctgttgcagg taatgcagca attcaccggg
    11161 atgaacgtca tcatgtatta cgcgccgaaa atcttcgaac tggcgggtta taccaacacc
    11221 acagagcaaa tgtgggggac agtgattgtc ggcctgacca acgtacttgc cacctttatc
    11281 gcaatcggcc ttgttgaccg ctggggacga aaaccaacgc taacgctggg cttcctggtg
    11341 atggctgctg gcatgggcgt actcggtaca atgatgcata tcggtatcca ctctccgtcg
    11401 gcgcagtatt tcgccatcgc catgctgctg atgtttattg tcggttttgc catgagtgcc
    11461 ggtccgctga tttgggtact gtgctccgaa attcagccgc tgaaaggccg cgattttggc
    11521 atcacctgct ccactgccac caactggatt gccaacatga tcgttggcgc aacgttcctg
    11581 accatgctca acacgctggg taacgccaac accttctggg tgtatgcggc tctgaacgta
    11641 ctgtttatcc tgctgacatt gtggctggta ccggaaacca aacacgtttc gctggaacat
    11701 attgaacgta acctgatgaa aggtcgtaaa ctgcgcgaaa tcggcgctca cgattaatct
    11761 cctcaggctt cctcccgtcg cggaggaagc cgcctcttgc agtcatcttt tcttcgctct
    11821 atcctctgcc gctatgaaaa catcccgtct ccctatcgcc atccaacagg ccgttatgcg
    11881 cggcctgcgg gaaaaactcg cccaggccaa cctgaagcta gggcgtaact acccggagcc
    11941 aaaactctct tacacccagc gcggaacctc cgccggaaca gcctggctgg aaagctatga
    12001 aatccgcctc aatcccgttt tgctgttgga aaacagtgaa gcttttattg aagaagtggt
    12061 accgcacgaa ctggcacatt tgctggtatg gaaacatttc ggccgcgtag cgccacatgg
    12121 caaagagtgg aagtggatga tggaaagcgt gctgggtgtt cccgcccgtc gtacgcatca
    12181 gttcgaactg caatccgtgc gtcgcaacac cttcccctac cgctgcaagt gccaggagca
    12241 tcagcttacc gtacgccgcc ataatcgcgt agttcgtggc gaggccgtct atcgctgtgt
    12301 tcactgcggt gaacagctgg ttgcgaaata accatctgaa ctatcaggaa ctttcctgat
    12361 ctggctgatt gcataccaaa acaactttcg ctacgttgct ggctcgtttt aacacggagt
    12421 aagtgatgta ccgttatttg tctattgctg cggtggtact gagcgcagca ttttccggcc
    12481 cggcgttggc cgaaggtatc aatagttttt ctcaggcgaa agccgcggcg gtaaaagtcc
    12541 acgctgacgc gcccggtacg ttttattgcg gatgtaaaat taactggcag ggcaaaaaag
    12601 gcgttgttga tctgcaatcg tgcggctatc aggtgcgcaa aaatgaaaac cgagccagcc
    12661 gcgtagagtg ggaacacgtc gttcccgcct ggcagttcgg tcaccagcgc cagtgctggc
    12721 aggacggtgg acgtaaaaac tgcgctaaag atccggtcta tcgcaagatg gaaagcgata
    12781 tgcataacct gcagccgtca gtcggtgagg tgaatggcga tcgcggcaac tttatgtaca
    12841 gccagtggaa tggcggcgaa ggccagtacg gtcaatgcgc catgaaggtc gatttcaaag
    12901 aaaaagctgc cgaaccacca gcgcgtgcac gcggtgccat tgcgcgcacc tacttctata
    12961 tgcgcgacca atacaacctg acactctctc gccagcaaac gcagctgttc aacgcatgga
    13021 acaagatgta tccggttacc gactgggagt gcgagcgcga tgaacgcatc gcgaaggtgc
    13081 aaggcaatca taacccgtat gtgcaacgcg cttgccaggc gcgaaagagc taacctacac
    13141 tagcgggatt ctttttgtta acccctaccc cacgcgtaca accgcgtggg gagacgacgc
    13201 ggatttttaa ctatgcgtat cccccgcatt tatcatcctg aaccactgac cagccattct
    13261 cacatcgcgc tttgcgaaga tgccgccaac catatcgggc gcgtactgcg catggggccg
    13321 gggcaggcgt tgcaattgtt tgacggtagc aaccaggtct ttgacgccga aattaccagc
    13381 gccagcaaaa aaagcgtgga agtgaaggtg ctggaaggca agatcgacga tcgcgaatct
    13441 ccgctgcata ttcacctcgg tcaggtgatg tcgcgtggtg aaaaaatgga atttacgatc
    13501 cagaaatcga tcgaactcgg tgtaagcctc attacgccac ttttttctga gcgctgcggc
    13561 gttaaactgg atagtgaacg tctgaacaag aagctccagc agtggcagaa gattgcaatt
    13621 gctgcctgtg agcagtgtgg tcgtaaccgg gtgccggaaa tccgtccagc gatggatctg
    13681 gaagcctggt gtgcagagca ggatgaagga ctgaaactga atcttcaccc gcgcgccagt
    13741 aacagcatca atacgttgcc gttaccggtt gaacgcgtcc gcctgctgat tggcccggaa
    13801 ggcggtttat cggcagatga aattgccatg actgcccgct atcaatttac tgatatcctg
    13861 ttgggacctc gcgttttgcg tacagagaca actgcgctca ccgccattac cgcgctacaa
    13921 gtacgatttg gcgatttggg ctaacggaga agaataatga tcaagctcgg catcgtgatg
    13981 gaccccatcg caaacatcaa catcaagaaa gattccagtt tcgctatgtt gctggaagca
    14041 cagcgtcgtg gttacgaact tcactatatg gagatgggcg atctgtatct gatcaatggc
    14101 gaagcccgcg cccatacccg cacgctgaac gtgaagcaga actacgaaga gtggttttcg
    14161 ttcgtcggtg aacaggatct gccgctggcc gatctcgatg tgatcctgat gcgtaaagac
    14221 ccgccgtttg ataccgagtt tatctacgcg acctatattc tggaacgtgc cgaagagaaa
    14281 gggacgctga tcgttaacaa gccgcagagc ctgcgcgact gtaacgagaa actgtttacc
    14341 gcctggttct ctgacttaac gccagaaacg ctggttacgc gcaataaagc gcagctaaaa
    14401 gcgttctggg agaaacacag cgacatcatt cttaagccgc tggacggtat gggcggcgcg
    14461 tcgattttcc gcgtgaaaga aggcgatcca aacctcggcg tgattgccga aaccctgact
    14521 gagcatggca ctcgctactg catggcacaa aattacctgc cagccattaa agatggcgac
    14581 aaacgcgtgc tggtggtgga tggcgagccg gtaccgtact gcctggcgcg tattccgcag
    14641 gggggcgaaa cccgtggcaa tctggctgcc ggtggtcgcg gtgaacctcg tccgctgacg
    14701 gaaagtgact ggaaaatcgc ccgtcagatc gggccgacgc tgaaagaaaa agggctgatt
    14761 tttgttggtc tggatatcat cggcgaccgt ctgactgaaa ttaacgtcac cagcccaacc
    14821 tgtattcgtg agattgaagc agagtttccg gtgtcgatca ccggaatgtt aatggatgcc
    14881 atcgaagcac gtttacagca gcagtaaccc accttagcga gaaggatctc gttgagactc
    14941 tgagtgacag cgcccttctt tccacgcata ctgggcgctg ttgctttttt gaaccaggaa
    15001 acagaacctc tgacaatgaa tttacagcat cactttctta ttgccatgcc tgctctccag
    15061 gatccgattt tccgtcgttc cgtggtctac atttgcgaac ataataccaa tggtgcaatg
    15121 gggatcatcg tcaacaagcc gctggaaaat ctcaaaattg aagggattct ggaaaagctg
    15181 aagatcacgc cggagccgcg tgatgaatca atccgtctgg ataaaccggt tatgctcggc
    15241 ggtccgctgg ctgaagatcg cgggtttatt ttgcatactc cgccctccaa ttttgcttcc
    15301 agcattcgca tttcagacaa cacagtgatg accacttccc gcgatgtgct ggaaacgctc
    15361 ggcaccgata aacaaccgtc tgacgtattg gtggctctag gttatgcctc ctgggaaaaa
    15421 ggtcaactgg aacaagaaat tctcgataac gcgtggctaa cggccccggc ggatctgaat
    15481 attctgttca aaacgccgat tgccgaccgc tggcgcgagg cggcaaaact gattggtgtg
    15541 gatattctca ccatgcctgg tgtggcaggg cacgcctgat gagtggaacc ttactcgcct
    15601 tcgacttcgg caccaaaagc attggcgtag cggtcggcca acgcattacc ggcactgctc
    15661 gccctttgcc tgcaattaaa gcacaggacg gtacgccgga ctggaacctc atcgaacgtt
    15721 tactgaaaga gtggcagccg gacgaaatca tcgtcggttt gccgctgaat atggacggca
    15781 ccgagcaacc attgactgcc agagcgcgta aatttgccaa ccgtattcat ggtcgtttcg
    15841 gtgttgaagt aaagctccat gacgaacgtc ttagcaccgt ggaagcccgt tccggtctgt
    15901 ttgaacaggg cggctatcgg gcgctcaaca aaggcaaagt cgactctgcc tctgcggtta
    15961 ttattctcga aagctatttc gagcagggat attaaggcga tttaaaagcc tggcggatag
    16021 tcgttaccgc catcttgccg gatgcggcgt gaacgcctat ccggcctaca tgtggcagcg
    16081 attgtaggtc tgataagacg cgcaaacgtc tcatcagacg tttattgcct ttgtaggccc
    16141 gataagcttg cacatcgggc atggcaacgt cacaaacgcc cttcccccac tcgctgctga
    16201 aaactctgct gaaacgttat catccccacc tgctgcccgg tttgaataac atgcggtaac
    16261 tggtgggttt tcccttcgcg aatcaaattc cccaccgcgg gagtgttaat caataattca
    16321 aacagcgcca cgcgtccttc ctgtttatcc acttccagct tttgtgacaa cacggcccgt
    16381 aaactacctg ccagttgatt acgtacggga tctttttcct gcgccggaaa tgaatccacc
    16441 agtcgctcaa ctgcctgcgc ggcaccacgc gtatgtaatg ttgccagcac caaatgcccg
    16501 gtttctgccg ccgtcagtgc cagacggatt gtctcgctgt cacgcagctc tccgagcaaa
    16561 atcacatcag gatcttcccg caatgcggcc cgcaatcccg atgcgaacgt catacagtgc
    16621 aaaccaattt cccgcttctg gatcaaacat cgctggctgg catagatata ttcaacagga
    16681 tcttccagcg tcagaatatg cgcatcggca tgttgattga gatagccaac catcgccgcc
    16741 agcgtggtag atttgctgct ccccgtcgcc cccgtcacca gaatcaggcc attctcactc
    16801 ttgagtaatt ccggcaatac cgttggtgcg ccaagctgtt cgagctgcgg gcagtgcgaa
    16861 ggtaacagcc gtaacgccag cgaaatgcca tgccgttgcg caaatgcgct gccacgcaat
    16921 cgctggtttt ccgccagcga cacggcaaaa tccagctggc cattttccag caatattgcc
    16981 cgctgatcgt catccagcca ctcccgcagt agctcttcga cgtccggcgc ctcaaacggc
    17041 gcagcttcca ttagcccgcg aatgcgccat cgtgcgggcc aggcgctgca caggtgtaga
    17101 tccgagacgt tatgctttac actaagggcc acaatttctt ccatattcat actaagatcc
    17161 tcggaaaatg aacgatattg cgcataacct ggcacaggtc cgggacaaaa tctcagcggc
    17221 tgcaacgcgt tgcggccgtt ctccagaaga aattacgctg cttgcagtca gtaaaacaaa
    17281 acctgcgagc gccatcgcag aagccattga tgccgggcag cgtcaatttg gtgaaaacta
    17341 cgttcaggaa ggggtagata aaattcgcca ctttcaggaa ctgggcgtaa caggattaga
    17401 atggcatttt attggcccgt tgcagtctaa taaaagccgc ctggtggcag agcatttcga
    17461 ctggtgtcat accatcgacc gtttgcgcat tgctacccgc ctcaacgatc agcgcccggc
    17521 agaacttccc cctcttaacg tactgattca aattaatatt agtgatgaaa acagtaagtc
    17581 cgggattcaa ctggcagaac tggacgagct ggcagcggcg gtcgctgaac taccgcgttt
    17641 acgtctgcgc gggctgatgg caatccctgc gcctgagtca gaatatgtaa ggcagtttga
    17701 agttgcacgc caaatggctg tagcatttgc cggactgaaa acgcgctacc cgcatatcga
    17761 cacgctctct ctgggaatgt cggacgatat ggaagccgcc attgcggcag gtagcacgat
    17821 ggttcgtatc ggcactgcaa tttttggtgc gcgtgattac tctaaaaaat aaggaattaa
    17881 aggaacgcca tgaatacgtt gactttcctg ctttcaacgg tcattgagct gtataccatg
    17941 gtgctgttat tacgcatctg gatgcagtgg gctcattgtg atttttacaa ccccttctca
    18001 cagtttgtag tgaaggtaac gcagccaatt atcgggccac tgcgccgcgt tattccggca
    18061 atgggcccca ttgacagcgc ctcgctgctg gttgcctata ttctcagttt tatcaaagcc
    18121 atcgtgctgt ttaaagtggt gaccttcctg ccaatcatct ggattgctgg tttactgatt
    18181 ctgctgaaaa ccatcggcct gctgattttc tgggtcctgc tggtgatggc gattatgagc
    18241 tgggtaagcc agggtcgtag tccgattgaa tacgtgctga ttcagctggc cgatccgctg
    18301 ctgcgcccga ttcgccgcct gctaccggca atgggtggga ttgatttctc gccgatgatc
    18361 ctcgttctgc tgctgtatgt catcaatatg ggtgtcgcag aagtattaca ggcaaccgga
    18421 aacatgctgc tgccggggct gtggatggcg ttatgagtgc cgtaacagtt aatgatgacg
    18481 gtctggtttt acggctctat attcagccga aagccagccg tgattctatt gtcggtttac
    18541 atggcgacga agttaaagtc gccattaccg cgccgccggt tgacggccag ggcaacagtc
    18601 atctggtgaa gtttctcggt aagcaattcc gggttgccaa aagccaggtg gtgattgaaa
    18661 aaggcgaact tggccgccac aaacaaatta aaatcattaa tccgcaacaa atcccgccag
    18721 aaatcgcggc gttaattaat taggtatcct atgcaaaaag ttgtcctcgc aaccggcaat
    18781 gccggtaaag tgcgtgagct ggcgtcgctg cttagcgact tcggtcttga tatcgtggcc
    18841 caaacagacc tcggcgtaga ttccgctgaa gaaaccggcc tgacctttat cgaaaacgcg
    18901 attctgaaag cgcgctatgc ggcaaaagtg accggtttac cggcaattgc cgacgactct
    18961 ggtctggcgg tagatgcgct gggtggcgcg ccgggtattt actccgcgcg ttattccggt
    19021 gaagacgcga ccgatcaaaa gaatctgcaa aaactgttgg aaacactgaa agacgtaccg
    19081 gacgaccaac gtcaggcgcg tttccactgc gtgctggtat atctgcgtca cgcggaagat
    19141 ccgactccgc tggtatgcca cggcagctgg ccgggcgtga ttactcgcga accagcgggc
    19201 actggtggct ttggttatga tccaatcttc ttcgtacctt ccgaagggaa aaccgccgcc
    19261 gaactgaccc gcgaagaaaa gagcgccatt tcccaccgtg gtcaggcgtt gaaactgctg
    19321 ctggacgctt tacgtaatgg ttaaattacc gccgctgagt ctctacattc acatcccgtg
    19381 gtgcgtgcag aaatgcccgt actgcgattt caactctcac gcgttgaaag gagaagtgcc
    19441 gcacgacgat tatgttcagc atctgctttg cgatctggac aatgatgtgg cttacgctca
    19501 gggccgtgaa gtaaagacaa tctttattgg cggtggtacg ccgagcctgc tttccggccc
    19561 ggcgatgcaa acgctgctgg acggcgtgcg tgcgcgtttg ccgctggcag cggatgcaga
    19621 aattactatg gaagcgaacc ctggaacggt agaagccgat cgctttgtcg attatcagcg
    19681 tgctggtgtg aaccgcatct ctattggtgt gcagagtttt agcgaagaaa agctgaaacg
    19741 acttgggcgt attcatggcc cgcaagaagc gaaacgcgcg gcaaagctgg cgagcgggct
    19801 ggggctgcgt agctttaacc tcgatttgat gcatggacta ccggatcaat cactggaaga
    19861 ggcgcttggc gatctacgcc aggccattga actgaatccg ccgcatcttt cctggtatca
    19921 actgaccatc gaacccaata cgctgtttgg ttcgcgacca ccggtgctgc cggacgataa
    19981 cgcgctgtgg gatattttcg aacaggggca tcagttatta accgcagcgg gttatcagca
    20041 atatgaaact tccgcttacg ccaaacccgg ttatcagtgc cagcacaatc tcaactactg
    20101 gcgatttggt gactacatcg gtattggctg cggcgcgcac ggcaaagtca ccttcccgga
    20161 tgggcgcatt ctgcgtacca ctaaaacgcg tcatcctcgt ggttttatgc aaggaaggta
    20221 tctggaaagc cagcgtgatg tcgaagccgc agataagccg tttgagttct ttatgaatcg
    20281 cttccgtctg ctggaggccg cgccgcgcgt ggagtttatt gcgtataccg ggctttgcga
    20341 agatgtgatt cgcccacagt tagacgaggc gattgcccag ggttatctca ccgaatgtgc
    20401 ggattactgg cagataaccg agcacggtaa attattctta aactcacttc ttgaattatt
    20461 cctcgctgag taatcattaa tgcgcctgcg ggcgcattaa tcacttcttt tccccccgtt
    20521 aattaacttc ggcaaaatat atttcttact gaataaacat cgatgatatt tattagatat
    20581 atctagtgaa tatttactca tcatttattg attataaaca tgtacaacct ggcgcacaaa
    20641 tttaatattt aatttctgac tttatttcaa gttgtgataa acatagacaa gttgtcagac
    20701 aggttaacca atttccatcc ttcaacaggg agttttttta tgaaaatgag gtcatttacc
    20761 cgctcattag tctgcgcatc cttgctggct ctggttagca ctggcgtaaa cgccgcagaa
    20821 aaagtgacgt taaagttagc acacaacctg gaaagaagtc atgttgtaca tcagagtttt
    20881 gaagagctgg cgaaagaggt aaaacagctg tcagaaggta atatggtgat ccgtatttat
    20941 cccagcagcc agatgggtaa tgcgcgagaa actatggaat tgttgcaaaa tggcgcactg
    21001 gatatgacca aaggttccgc cagtgatctg gagtcttttg ataatattta tgctatctat
    21061 aatttgccgt tcttatttaa agatcaggct cacttcaaca aagttgtttt tggtgaagtt
    21121 ggtaaagaaa ttatggactc aacaaaagac aaaggctttt ttgctttatc agcttatgta
    21181 gcgggtacac gcagtttcta tgcgaaaaaa ccgattacta agccagaaga tcttaaaggg
    21241 ctgaaaattc gtgttcagcc aagtccgacc accatcaaaa tgattgaact gatgggtggt
    21301 tcacccactc caatttcatt tggcgaagtt tatactgcga tgcagcaagg tgtagtagat
    21361 ggcgcagaaa ataacgtgcc ttcctgggtg cagactcgac atattgaaat tgccaaagtt
    21421 ttttctgaag atgaacacgc ctcaattcct gactttttgg ttatctccac caaaacatgg
    21481 aataaattaa ccccagaaca gaaacaaatc ctcgaaacag cagcgaagaa atccgaagct
    21541 tatcaacaaa aattgtggga aaaaattgac gctgacacac gtgctcaggc gaaagctatg
    21601 gggggtgaaa ttgtaaaagt ggataaagca ccattccgtg ccgcagttca gcctctgttt
    21661 gacgacttca aaaaagatcc aaaacaagct gctttgttag agaagttcga caacgctgct
    21721 caataatatt ccgggccagt ctctggccct gattttccaa gatgcttaca atgatattta
    21781 accgcctgaa acttgcggta gaccgcgtca ttgccgcgtt ttctgtcgcc gtcatgcttg
    21841 ctctggttgt atgcgtagtc tggcaagtat ttagccgcta cgtacttaac cagccaagta
    21901 cattaaccga tgagctggca cgatttttga tgatctgggt agggttatta ggtgc
//
LOCUS       DAZPDU010000060        21643 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZPDU010000060 DAZPDU010000000
VERSION     DAZPDU010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21643
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.060"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..1127)
                     /gene="fsr"
                     /locus_tag="ACHURS_002066"
     CDS             complement(<1..1127)
                     /gene="fsr"
                     /locus_tag="ACHURS_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFR1237806.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIAD"
     gene            1345..2997
                     /gene="ushA"
                     /locus_tag="ACHURS_002067"
     CDS             1345..2997
                     /gene="ushA"
                     /locus_tag="ACHURS_002067"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFR1237807.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMIGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(3034..3513)
                     /gene="ybaK"
                     /locus_tag="ACHURS_002068"
     CDS             complement(3034..3513)
                     /gene="ybaK"
                     /locus_tag="ACHURS_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFR1237808.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMAAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(3717..4511)
                     /locus_tag="ACHURS_002069"
     CDS             complement(3717..4511)
                     /locus_tag="ACHURS_002069"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFR1237809.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQITMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            4649..4990
                     /locus_tag="ACHURS_002070"
     CDS             4649..4990
                     /locus_tag="ACHURS_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFR1237810.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(5205..7709)
                     /gene="copA"
                     /locus_tag="ACHURS_002071"
     CDS             complement(5205..7709)
                     /gene="copA"
                     /locus_tag="ACHURS_002071"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFR1237811.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARVVTADGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKTGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            7971..8903
                     /gene="glsA"
                     /locus_tag="ACHURS_002072"
     CDS             7971..8903
                     /gene="glsA"
                     /locus_tag="ACHURS_002072"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFR1237812.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            8906..10198
                     /locus_tag="ACHURS_002073"
     CDS             8906..10198
                     /locus_tag="ACHURS_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFR1237813.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            10229..10326
                     /locus_tag="ACHURS_002074"
                     /pseudo
     CDS             10229..10326
                     /locus_tag="ACHURS_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10323..10730
                     /gene="cueR"
                     /locus_tag="ACHURS_002075"
     CDS             10323..10730
                     /gene="cueR"
                     /locus_tag="ACHURS_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFR1237814.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(10731..11189)
                     /locus_tag="ACHURS_002076"
     CDS             complement(10731..11189)
                     /locus_tag="ACHURS_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFR1237815.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(11186..12103)
                     /locus_tag="ACHURS_002077"
     CDS             complement(11186..12103)
                     /locus_tag="ACHURS_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFR1237816.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGTSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            12249..12926
                     /gene="fetA"
                     /locus_tag="ACHURS_002078"
     CDS             12249..12926
                     /gene="fetA"
                     /locus_tag="ACHURS_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFR1237817.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNRQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            12913..13692
                     /gene="fetB"
                     /locus_tag="ACHURS_002079"
     CDS             12913..13692
                     /gene="fetB"
                     /locus_tag="ACHURS_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFR1237818.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(13755..14609)
                     /gene="cnoX"
                     /locus_tag="ACHURS_002080"
     CDS             complement(13755..14609)
                     /gene="cnoX"
                     /locus_tag="ACHURS_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFR1237819.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNFTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGMVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(14670..15479)
                     /gene="ybbO"
                     /locus_tag="ACHURS_002081"
     CDS             complement(14670..15479)
                     /gene="ybbO"
                     /locus_tag="ACHURS_002081"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFR1237820.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(15469..16092)
                     /gene="tesA"
                     /locus_tag="ACHURS_002082"
     CDS             complement(15469..16092)
                     /gene="tesA"
                     /locus_tag="ACHURS_002082"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFR1237821.1"
                     /translation="MNFNNVFRWHLPFLFLALLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            16063..16749
                     /gene="ybbA"
                     /locus_tag="ACHURS_002083"
     CDS             16063..16749
                     /gene="ybbA"
                     /locus_tag="ACHURS_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFR1237822.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            16746..19160
                     /gene="ybbP"
                     /locus_tag="ACHURS_002084"
     CDS             16746..19160
                     /gene="ybbP"
                     /locus_tag="ACHURS_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFR1237823.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYDKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMQLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
     gene            complement(19394..20488)
                     /gene="mnmH"
                     /locus_tag="ACHURS_002085"
     CDS             complement(19394..20488)
                     /gene="mnmH"
                     /locus_tag="ACHURS_002085"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFR1237824.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQAAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTDSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKTQ"
     gene            complement(20557..21483)
                     /gene="allS"
                     /locus_tag="ACHURS_002086"
                     /pseudo
     CDS             complement(20557..21483)
                     /gene="allS"
                     /locus_tag="ACHURS_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
BASE COUNT         5014 a         5685 c         5799 g         5145 t
ORIGIN      
        1 tgatcggcga taagccccag aactgccgct cccagacctc ccatgccaaa agcaaaaccg
       61 aaaaagagtc cagaaaccat accgatacgt cccggaagta gctcctgagc gtagaccaga
      121 atggcagaga atgccgaagc gaggataaat ccaataatca ccgttaaaac ccccgtccag
      181 tgcaggctgg cgtagggtaa aatcagcgta aacggcgcaa cgccgaggat agagccccaa
      241 atcacatatt tccgtccaat tttatcccct acaggcccgc cgatcaccgt acctgccgca
      301 acggcaaaca ggaaggcaaa cagatgaagc tgggcattct ggatagataa tccgaatttt
      361 tgcatcagat aaaaggtgta atagctgctg atgctcgcca tatagaaata tttcgagaaa
      421 atgaggatta acagaatgct gactgccagt accactttgt tgcgcggcag tggattgatt
      481 atcgtcgctt tgggttttcc tttattcatt cggtgctgtg ccgagtacca acggctaatt
      541 tgcgccaaca ccacgatcgc cagcagtgcc gcaagcacaa accaggcaac gttgcctttg
      601 ccataaggcg cgataatcac cgccgccagc aagggtccca gggaactgcc aaagttgccg
      661 ccgacctgaa agatagattg cgccaggcca tgccgcccgc cggaagccat acgggctacg
      721 cgagaagatt ccggatgaaa gaccgatgaa ccggtaccga ccagcgccgc cgccagcaga
      781 actgcgccaa aactgcccgc cagcgcaagc agcaccagac cacttaaggt aaagcacatg
      841 ccaattggca acgaccacgg catcggatat ttatcggtcc agtagccgac cactggttgc
      901 agtagcgaag aggcgagctg gaaggtgagg gttatcatgc caatctgcat aaatgtcaga
      961 gaaaattctg actgaagcag cggataaatc gccagaatca gcgattggat catgtcgttc
     1021 agcagatgtg agaggctgat agcacctaaa ataccaaacg atgttcgggc cttggtcgtt
     1081 gacgcagccg cgcccgccac aggctggggt tgttcactca ttgccatagg aaagtcactt
     1141 tttcagggtt gcgatgtaaa gaatgatctt atttgtgatt attacccgac taacatacct
     1201 gtatgcgtcg tctgaaggaa gtctcaacgc cgaatacaga atttctaatc tggatgcaga
     1261 tttatcttca ccggacgcag acttgtctat gatgtcgcgt catactattt ttcaacacgt
     1321 tgaaatcagg tcagggagag aagtatgaaa ttattgcagc ggggcgtggc gttagcgctg
     1381 ttaaccacat ttacactggc gagtgaaact gctctggcgt atgagcagga taaaacctac
     1441 aaaattacag ttctgcatac caatgatcat catgggcatt tttggcgcaa tgaatatggc
     1501 gaatatggtc tggcggcgca aaaaacgctg gtggatggta tccgcaaaga ggttgcggct
     1561 gaaggcggta gcgtgctgct actttccggt ggcgacatta acactggcgt gcccgagtct
     1621 gacttacagg atgccgaacc tgattttcgc ggtatgaatc tggtgggcta tgacgcgatg
     1681 gcgatcggta atcatgaatt tgataatccg ctcaccgtat tacgccagca ggaaaagtgg
     1741 gccaagttcc cgttgctttc cgcgaatatc taccagaaaa gtactggcga gcgcctgttt
     1801 aaaccgtggg cgctgtttaa gcgtcaggat ctgaaaattg ccgttattgg gctgacaacc
     1861 gatgacacag caaaaattgg taacccggaa tacttcactg atatcgaatt tcgtaagccc
     1921 gccgatgaag cgaagctggt gattcaggag ctgcaacaga cagaaaagcc agacattatt
     1981 atcgcggcga cccatatggg gcattacgat aatggtgagc acggctctaa cgcaccgggc
     2041 gatgtggaga tggcacgcgc gctgcctgcc ggatcgctgg cgatgatcgt cggtggtcac
     2101 tcgcaagatc cggtctgcat ggcggcagaa aacaaaaaac aggtcgatta cgtgccgggt
     2161 acgccatgca aaccagatca acaaaacggc atctggattg tgcaggcgca tgagtggggc
     2221 aaatacgtgg gacgggctga ttttgagttt cgtaatggcg aaatgaaaat ggttaactac
     2281 cagctgattc cggtgaacct gaagaagaaa gtgacctggg aagacgggaa aagcgagcgc
     2341 gtgctgtaca cccctgaaat cgctgaaaac cagcaaatga tctcgctgtt atcgccgttc
     2401 cagaacaaag gcaaagcgca gttggaagtg aaaataggcg aaaccaatgg tcgtctggaa
     2461 ggcgatcgtg acaaagtgcg ttttgtacag accaatatgg ggcggttgat tctggcagcc
     2521 caaatggatc gcactggtgc cgactttgcg gtgatgagtg gaggaggaat tcgtgattct
     2581 atcgaagcag gcgatatcag ctataaaaac gtactgaaag tgcagccatt cggcaatgtg
     2641 gtggtgtatg ccgacatgat cggtaaagag gtgattgatt acctgaccgc cgtcgcacaa
     2701 atgaagccag attcaggtgc ctacccgcaa tttgccaacg ttagctttgt ggcgaaagac
     2761 ggcaaactca acgacctgaa aatcaaaggc gaaccggtcg atccggcgaa aacttaccgt
     2821 atggcgacat taaacttcaa tgccaccggc ggtgatggct atccgcgcct tgataacaaa
     2881 ccgggctatg tgaataccgg ctttattgat gccgaagtgc tgaaagcgta tatccagaaa
     2941 agctcgccgc tggatgtgag tgtttatgaa ccgaaaggtg aggtgagctg gcagtaatcc
     3001 gaaagtgccg gatatttgca tccggcacaa tgcttaatcg cggcgggcga tatcagcaaa
     3061 tttggcatcg aggatcttcg ccagatcgcc tgccgccagt tcgatatcca gtccgcgctt
     3121 gccgccagaa acataaatag tggcaaattc ttgtgcgggg gcgtcgataa tcgtcggcag
     3181 acgttttttc tgccccagtg ggctaatccc cccaaccagg tatcccgtcg aacgctgcgc
     3241 ggccatcgga tcggccatct caactttctt ggcacccagc gcttttgcta cttttttaag
     3301 atccagttga cctgcgaccg gcgtaacggc cactgccaga tgtttcatat caccgttcac
     3361 tgccaccagt agcgttttgt agacttgatc cggattcaaa cctaattttt tgacgacttc
     3421 atcgccaaaa ttggtttcag ccggatcgtg ctcgtaggta tggatttgaa acgaaatctt
     3481 gtttttttcg agtaatttaa ctgcgggtgt catggtaatt ttcctgcctt aaacaaaaaa
     3541 acgaagcaag catacgcctg atggcggtcc aaaaaaaaga gtcatcttgc ctaagagtat
     3601 tggcaggatg gtgagattga gcgacaatcg agttacaccg tcgcttaaag tgacggcata
     3661 ataataaaaa aatgaaattc ctctttgacg ggccaatagc gatattggcc atttttttag
     3721 cgcaacattt gcggcaaatt cccttctcca tacaggtgta gtgcaccgac cgcgaccaca
     3781 tatcgccccg gcggcatggc gcgtaattta tcccgccagg cgagatttcg ctgatgcatc
     3841 agcacatcgt acagcgactg actgaacgta ttgggcagcg ttatatcatt attttgcgga
     3901 ggtgcattca gccaccagct catcatttgt tgcagcaacc gtgcgttggt atgccagtgg
     3961 gtcagcgtat cgtccagcag cgccagtcct ttgtcaggga gctggagcaa catggtaatc
     4021 tggttttcag ccccttccag ttcaatcacg ggtttatgtt gttgcttcgc cgcctgcaat
     4081 aactggtaat cgataccgta ttctgcccgc agccccagtt tttgtgcctg cgtcgcctga
     4141 agaaccatcg cgatttgcca cagcggttga gtagaaaaga gcgacggaga aatgcccatc
     4201 tcctggctaa tgtgctgcag attttggagt tgctcctcgt taatgcgctc ttccagcgcc
     4261 tcgcaagtag gcaaattagc aaaaggcgta tcgctggtgg aaacatccgc ctcgacgatc
     4321 agcgcatcgg cgtttttgag ctttttgagc aaacgggtgg gcaggggagc catatcgtgg
     4381 ctacccatat gaatactgcc aatcagatga aaatggcgat tgccgggaag ggtgatatcg
     4441 atggcaggcc aggtgtaatg attaccgcgc agcgcggccc aaagcgtttt tacccggtac
     4501 aacagatcca tacgacctcc ctttgtgaaa tatcatgcta gcgcgcggtg agggatggcg
     4561 caaatggagt tggggacttg tttaatgtgt ttgtatgatt cagtatgttc ttgcatcgct
     4621 attcacaggg aagcaacagt taaaaaccat gaaacaggca acaagaaaac cgacgacacc
     4681 cggagatatt cttctctatg aatatctgga accgctcgat ttgaaaatca atgagttagc
     4741 agagttgctg catgttcatc gtaatagcgt cagtgcactg atcaataaca atcgtaaact
     4801 caccactgag atggcattcc gtctggcgaa agtttttgat accacagtcg attttaggct
     4861 aaacctccag gcggcagttg atctttggga agttgaaaac aacatgcgca cccaggaaga
     4921 attgggacgg attgaaacag tggctgaata tttggcacgc cgtgaagagc gtgcaaaaaa
     4981 ggtcgcgtaa ggccaaaaca tcgcttcatc tcgtaggccg gataaggcgt tcacgccgca
     5041 tccggcatcc gagcaccatt gcctgatgcg acgcttaccc gtcttatcag gcctacaaac
     5101 ctgtgcctga accgtaggcc ggataaggcg tttacgccgc atccggcagt catgcgtcga
     5161 tgccaaatgc gccgccctaa agcagcgcat ccgcaatgac gtacttattc cttcggttta
     5221 aaccgcagca accggttggc gttactcact acggtaatcg acgagagcgc cattgccgct
     5281 ccggcaacta ccgggttaag cagtgttccc gtaaacggcc acaaaatacc ggcggcgacc
     5341 ggaataccga tgctgttgta gataaaggca ccgagcaggt tctgcttcat gttgcgcagc
     5401 gttgcgcggg aaatcgcgag cgcatccgca acgcccatca ggctatggcg catcagggta
     5461 atcgccgcgg tttcaatggc aacatcactg ccgccaccca tcgcaatgcc gacatccgcc
     5521 tgggccagcg ctggcgcgtc gttaatgccg tcgcccacca ttgccacctg acgtccttca
     5581 ctttgcagac gtttgatcgc ttcggcttta ccgtccggca gcactccggc aatcacttca
     5641 tcaattccag cttctttggc gatcgcattg gcggtggttg ggttatcccc ggtcaacatc
     5701 accagacgat atcccgtttt atgcaggcgt tgcagcgccg ccacgctatc actacgcaac
     5761 ggatcgcgta cagccagcag ggctactgct ttcccgtcaa tcgccagcag cacaggcgtt
     5821 gctccttgcg atgcctgagc agtaatctcc gcttcgatag ctttggtacc aacctgttgc
     5881 tcatttaaca gcgcctgatt gcccagcaat aacgcatgac cttcagcttc accgctcacg
     5941 cccagcccgc gcaatgtgcg gaaaccgttg acctgcggca gctgcatatc acctgcttta
     6001 tcgaggatcg cccgtgccag cggatggctg gaaccttgct ccagtgccgc cgccagacgc
     6061 aatgcctgcg cttcatcaac atcagcaaac gttttcactg cgacaacctg cggcttccct
     6121 tcggtcagcg tcccggtttt atcgaacacg acggtatcga gtgtactggc gcgttgcagc
     6181 gcgtcggcgt cccgcaccag cacgccaaac tcagccgccc gcccgacgcc ggaaataatc
     6241 gacatcggcg tcgccagccc cagcgcacac ggacaggcga taatcagtac cgtggtggca
     6301 atcaccaggg tataaacaat ttgcggtgcc ggaccaaaga agtaccagat tgccgcactg
     6361 accagcgcaa tcaccaccac cacaggtaca aatacggctg agattttatc cgccagctga
     6421 ccgatttctg gcttgctgct ctgggcctgg cgcaccatgc gaatgatccg tgacagcgtg
     6481 gtatggctgc caaccgcact ggcacgaaac agcacactgc cgtcctgtac cactgtcccg
     6541 gcatggacgc tttcgccttc gcctttttgc tgtgggattg gttcgcccgt cagcatcgct
     6601 tcatccagcc atgcttcgcc ctgggtaatc tcgccatcta ccggcacgcg atcgccggtc
     6661 gtcaggcgca gtaacatccc tggctgaact tccgccagcg gcacgctttt ttcaccatct
     6721 gcggtgacca ctcgcgccgt cggtggggtt aaatcgagta atttctccag cgccttagaa
     6781 gaacgctgac gtgcgcgcgc ttccagcata tggccgagat taatcagacc gataatcatc
     6841 gcactggctt catagtaaag atgccgcgct tccatcggga accactgcgg ccacaggttg
     6901 acgctcatcg aatagagcca cgccacaccc gttcccagcg ccaccagcgt atccatcgtc
     6961 gctgcaccgt tcagcaggct tttccatgca ctgcggtaaa aatggccgcc ggcgaaaacc
     7021 atcactgcca gggttatcag gccgataact aaccacaagc tgcggttgtc agcggtgacc
     7081 atcatgttat cgccgatcat cccccagacc attaccggga tacccacagc cagtgcaaca
     7141 attgcctgcc agcggaagcg cttcatcgta gctacggcgg tttcttgctg gcgctcgcgg
     7201 cgtttagcgt catcttcaat cgcttccgca ccgtagcccg ctttttccac cgcctgcact
     7261 aaatcttgtg gggaggcact gcccatcacc agcgcagtac gctccgccag gtttacccgt
     7321 gcctgagtga cgcccggtac gctttgcagc gcattttgta cgcgggtgac acagctggcg
     7381 cagctcatgc cgctcagcag caactgctgg ctgtcatcgt catcggcggt cgctgccgga
     7441 agcgcctcag aaaccgctgt cagtgcttcc gacgggattg atgactccgc cagcggttta
     7501 gcctttgggt ggcttacaga tgcgtcataa cccgcttgtt tgatggtttc gatcagctgt
     7561 tctgcactgg cagtcccggt aacgtgcgct tcagtgatag acacatccgc ctgctcaaca
     7621 tccgggcgct gttcaagact ttctttcacg cgtttaacgc agtgaccgca ggacaggccg
     7681 tccagggtca ggtcgatagt ttgtgacata aaacactcct ttaagacagt tttgactggc
     7741 tgtgataaag gttaaacctt ccagcaaggg gaaggtcaag aaattaataa accaggcggg
     7801 taaaagtccg taaagattaa aaaatcggct cgatttgcat caggattaga catttatttc
     7861 tttgttttcc tgtagttaag ttgcgggtgc taagttaaat caggatgcct gaaaatcggc
     7921 accggggtga ggaattacct cccgcatcta taaaaaggag ttaacaaaag atgttagatg
     7981 caaacaaatt acagcaggca gtggatcagg cttacaccca atttcactca cttaacggcg
     8041 gacaaaatgc cgattacatt ccctttctgg cgaatgtacc aggtcaactg gcggcagtgg
     8101 ctatcgtgac ctgcgatggc aacgtctata gtgcgggtga cagtgattac cgctttgcac
     8161 tggaatccat ctctaaagtc tgtacgttag cccttgcgtt agaagatgtc ggcccgcagg
     8221 cggtacagga caaaattggc gctgacccga ccggattgcc ctttaactca gttatcgcct
     8281 tagagttgca tggcggcaaa ccgctgtcgc cactggtaaa tgctggcgct attgccacca
     8341 ccagcctgat taacgctgaa aatgttgaac aacgctggca gcgaatttta catatccaac
     8401 agcaactggc tggtgagcag gtagcgctct ctgacgaagt caaccagtcg gaacaaacaa
     8461 ccaacttcca taaccgggcc attgcctggc tgctgtactc cgccggatat ctctattgtg
     8521 atgcaatgga agcctgtgac gtgtacaccc gtcagtgctc tacgctcatc aatactgttg
     8581 aactggcaac gcttggcgcg acgctggcgg cgggtggtgt gaatccgttg acgcataaac
     8641 gcgttcttca ggccgacaac gtgccgtaca ttctggccga aatgatgatg gaagggctgt
     8701 atggtcgctc cggtgactgg gcgtatcgcg ttggtttacc gggcaaaagc ggtgtaggtg
     8761 gcggtattct ggcggtcgtc cctggcgtga tgggaattgc cgcgttctca ccaccgctgg
     8821 acgaagaagg caacagtgtt cgcggtcaaa aaatggtggc atcggtcgct aagcaactcg
     8881 gctataacgt gtttaagggc tgatcatgat gaacacggaa ggtaataacg gtaacaaacc
     8941 cctcggtcta tggaacgtcg tttccatcgg tattggggca atggtggggg cggggatctt
     9001 cgcgctgctg gggcaggctg cgttgctaat ggaagcctcg acctgggtcg cctttgcttt
     9061 tggcggtatt gtggcgatgt tttccggtta tgcctatgcg cgtctggggg cgagctatcc
     9121 cagcaatggc ggtattatcg acttttttcg tcgcggatta ggcaacggcg tcttttcgct
     9181 agcgctctcg ttgctgtacc tgttgacgct ggcggtgagc atcgccatgg ttgcccgtgc
     9241 ttttggcgct tatgccgtgc agtttttgca tgaaggcagc caggaggagc accttatttt
     9301 gctctacgcg ttggggatca ttgcggtgat gacgcttttc aactccttaa gcaaccatgc
     9361 ggtagggcgg ctggaagtga tcctcgtcgg cattaaaatg atgatcctgt tattgctgat
     9421 tattgccggt gtctggtcgc tgcaaccggc gcatatttcc gtctctgcgc cccccagctc
     9481 cggtgcgttc ttctcctgta ttgggataac cttccttgcc tatgcgggct ttggcatgat
     9541 ggcgaacgcg gcggataaag tgaaagatcc gcaggtcatt atgccacggg cgtttctggt
     9601 ggcgattggc gttaccacgt tgctttatat ctcgctggcg ctggttttgc ttagcgatgt
     9661 atcggcatta gagttagaaa aatatgctga taccgccgta gcgcaggctg cttctccgct
     9721 gctcgggcat gtgggttatg tgatcgtcgt catcggcgct ttactggcga cggcttcagc
     9781 cattaacgcg aacctgttcg ccgtgtttaa catcatggac aacatgggca gcgaacgcga
     9841 actgccgaag ctaatgaata aatccctgtg gcggcagagt acctggggca acattattgt
     9901 cgtggtgttg attatgctga tgacggcggc gctaaattta ggctcactcg ccagtgttgc
     9961 cagcgccacc tttttgattt gctacctggc ggtgtttgtg gtggcgatcc gcctgcgtca
    10021 tgatattcac gcctcgttgc cgattcttat cgttggtacg ttggtgatgt tgttggtgat
    10081 cgttggcttt atctacagtc tgtggtccca gggtagccgt gcgttgatat ggattattgg
    10141 ctcactctta ctcagcctta ttgtggcaat ggtcatgaag cgcaataaaa ccgtataaca
    10201 tctctctgtg cgcagtactt cctgtattat tgtggtggcg gtcgatattc gcactggcaa
    10261 aaaaacgtgc ttgaatatct gttgaaaccc tttaacaaag cacaggaggc gttgcgcgaa
    10321 cgatgaacat cagcgatgta gcaaaaatta ccggcctgac cagcaaagcc attcgcttct
    10381 atgaagagaa ggggctggtg acgccgccga tgcgcagcga aaacggttat cgcacctaca
    10441 cgcagcagca tctcaacgaa ctgaccttac tgcgccaggc acggcaggtg ggctttaacc
    10501 tggaagagag cggcgagctg gtgaatctgt ttaacgaccc gcagcggcac agcgccgacg
    10561 tcaaacggcg cacgctggag aaggtggcgg agatcgaacg acacattgag gagctgcaat
    10621 ccatgcgcga ccagctgctg gcactggcga atgcctgccc tggcgatgac agcgccgact
    10681 gcccgattat cgaaaatctc tccggctgct gtcatcatcg ggcagggtga ttaagacgag
    10741 acggctcgga tatgtagggt tatcccttct atcgcaatga cttcaacatg cgtacctgca
    10801 ccgagatcct cgctggcgct gacaggccat gaactgtcac cgacgcgcat atgaccgcgc
    10861 ccgttgacca gcggagattc cagcacaaaa cgtcggccaa tcagctgctg cccgcgctgg
    10921 tttaaatgac tatcgctgtg cttttgttcg cgcacccgcc gcgacaacca tttccaccac
    10981 agccaggcgg cgagcagcgt caggatggca aacattaccc cttgccactc ccaacccagc
    11041 ggcaccagcc agaccaccag gccggtaatc accgctgcca cgccgctcca caacaaataa
    11101 ccatttccgc ccagcatctc ggctgccagc agcaaaccgc cgagactgag ccagaaaata
    11161 tgtggatgaa cgaccattaa ctccatcatg gctgagtccg tttgttggcg ctgtctttca
    11221 ccagctcggc aatcccggca atcgacccca tcaggctgct ggcctctaat ggcatcatca
    11281 ctactttgct gttactggag gtaccgatct gctgtaacgc ttcggtgtat ttctgcgcta
    11341 cgaagtagtt caccgcctga atatcaccgg aggcgatagc ttcagacacc attttggtgg
    11401 cgcgggcttc tgcttcggcg gaacgttcac gcgcttcagc ctgtaaaaac gccgactgac
    11461 gttcgccttc cgctttcagg atttgcgact gtttttcacc ttcggctttg aggatttccg
    11521 cctgacggat cccttccgct tcaagaatgt aagcgcgttt ggtacgttcc gctttcatct
    11581 gcgcgttcat tgaagagata agctctgccg gtgggcgcac gtcgcgaatt tcaatacggg
    11641 tgactttaat cccccacggg ttggtggctt catcgacaat acgcagcagg cgtgagttga
    11701 tgctgtcgcg ctgagagagc atttcgtcaa gttccattga acccaacacg gtacggatgt
    11761 tagtcatggt caggttgatg atcgccagct ccagattgct gacttcataa gccgcgcgcg
    11821 gcgcgtcaat cacctgaata aaacagactg cgtcgatggt aacgttggcg ttatctttcg
    11881 agataacttc ctgggaaggg atatcgagca cttgctccat catattgatc ttgcgaccaa
    11941 tgcgatccat aaacggcacc accagactga gccccggctg taacgttttg gtatagcgac
    12001 caaaacgttc taccgtccat tgatagccct gcggcacgat tttgacgccc gcgccgacaa
    12061 tgaccagcgc gacaaaaatg agaatcggga taaagataag catcggaaaa acctcctgtt
    12121 gtaccgtcca taatcagcaa aattgctgct tgattaaaca aattatacct gattactgaa
    12181 agagagttcc cccttattcc tgcgaaggat aaactgtttt tagtaaaaat cagaaaaagg
    12241 gaacagcgat gcaggaaaat agtcctttgc ttcagctaca aaacgtagga tatctggcgg
    12301 gtgatgcgaa gattcttaat aacatcaatt tttcgctgcg tgctggcgaa tttaagttaa
    12361 ttaccggtcc ttctggttgt ggcaaaagta cgctgctaaa aatagttgct tcattgatca
    12421 gcccaaccag cggaacgtta ctgtttgaag gtgaggatgt cagcacacta aagccagaaa
    12481 tctaccgcca acaagtctct tactgcgccc agacaccgac gctgtttggc gatacggtat
    12541 acgataatct gatctttccc tggcagatcc gtaaccggca gcctgaccca gccatttttc
    12601 tcgattttct cgaacgcttc gccttgccgg acagcatttt gacgaaaaat atcgccgagc
    12661 tatctggtgg tgaaaaacaa cgcatctcac tgattcgtaa cctgcaattt atgccgaagg
    12721 ttttattgct ggatgaaatc accagtgcgc tggatgaaag taataaacat aacgttaatg
    12781 agatgatcca tcgttatgtg cgcgagcaaa atattgccgt gctgtgggtg acacacgata
    12841 aagacgaaat taatcatgcg gataaagtga ttacactgca accgcatgcc ggagaaatgc
    12901 aggaagcacg ctatgaactc gcataatatt actaacgaat cattagcact ggcattaatg
    12961 ctggtggtgg tggcaatctt aattagccat aaagaaaaac tggcgctgga gaaagatatt
    13021 ctctggagcg tcggacgagc gataattcag ctgattattg tcggctatgt gctgaagtat
    13081 attttcagcg tggatgatgc cagcctgaca ttattgatgg tgttatttat ctgctttaat
    13141 gcggcgtgga acgcgcaaaa acgcagtaaa tatattgcta aagcttttat ctcatcgttt
    13201 attgccatta cggtcggggc gggaattacc ctggcggtgc tgattctctc agggtcgatt
    13261 gaatttatcc cgatgcaggt gatccctatc gccgggatga ttgccggtaa cgccatggta
    13321 gcggtggggt tgtgttacaa caatttaggg caacgggtca ttagcgaaca gcaacagatt
    13381 caggagaaac tgagtcttgg tgcgacgccg aagcaggctt cagcgatatt gattcgcgac
    13441 agtattcgcg cggctttaat tccgacggtc gattcagcaa aaacggttgg cttagtgagt
    13501 ttaccaggaa tgatgtccgg gctgatattt gccgggattg atccggtgaa ggcgattaaa
    13561 tatcagatta tggtgacctt tatgctgctc tcaaccgcca gcttgtcgac cattattgcc
    13621 tgctatttaa cctatcgtaa gttttataat tcgcgccacc agttggtggt gacgcaattg
    13681 aagaagaaat gatgcgatgc cggatgcgca gcatcgcatc cggcgttgtg gttcatgtgc
    13741 cgatcaacgg aatatcaata caacaatgca tacagctggc ggcgatactt cgacgccagt
    13801 gcatcacccg tacccagcgc agcgaggatc tcctggaacg ttttacgcgt ctgaccgtct
    13861 gcggcggtga gatctttacg cagatgcccg aacagcaact ccagcgcctc ttcattgcgc
    13921 ccaacctgat gcagttgcag cgccagttgc gtcgccagtg cggcatcttc tggattctcc
    13981 gccacctgct gttgcaactg ttgaatttcc ggcgtatcag ccgcctgctt cagcagttcg
    14041 atttgcgcca ccatcccctg gtagcgggtg tcctgatcct gtaacggaat ggttttcagc
    14101 accgcttctg catcttcaga acggttcagc gcaatcagcg tttctgccaa cagcaggccg
    14161 atctccccgt tctgattcga caactgccag gcgtctttca gcaatggcag ggcatcggtg
    14221 aaattgcctt cctgcatcag ttgcatcgcc tgctgcgctt tcagctcttc ttcgcgcggc
    14281 agcactttat ccagcagggc gcggatcgcc tcttccggtt gcggcccctg gaagccatct
    14341 accggttgcc cgttctggaa cagatacacc gtcggaatcg cgcgcagacc aaactgggcg
    14401 gcaatcatct gctccgcatc gcagtccagc ttcgccagaa taaattgccc gttgtactgc
    14461 gccgcaaggc tttccagaat tggggttaac tgcaaacagt gctggctacg ttcagaccaa
    14521 aaatagaaca gcaccggagt ggtcatcgac tgttcaagaa cctgttgcag gttagattcg
    14581 ttaatgttga caatattttc tacggacatg gagtcgctct ctgttgtcga tattttcttt
    14641 gacatggggg cttaagcgcg cgcttcaact cacccctgca atattttgtc catcacgcgc
    14701 cccggcagca ggcgcttaag caccattact gcccaagtca ccagcgttac cggatagcgc
    14761 atcttcggct tctcgctaat aaaagcatgg cgtactttgt ccaccaccgc ttccggtccc
    14821 aacgtaaagc gggcggcgat gccgggattt tcgactggtt tatcactttg cgtctggttg
    14881 acgttgtcgg tgaagcgagt acgaatggga ccgggttcga tcaggctgac tttaattccg
    14941 ctgtggcgta gctccatgcg cagcgcgtct gaccatgcct ccagcgcata tttactggcc
    15001 gcgtaagcgc cacgacccgg tgtggagatt aatcccatca ccgatgatgt catcacaata
    15061 cgcccttcac cgtgcggtaa catcgcgggt aacaggcgca tggtgagctg gtgtgcgccg
    15121 aaaaagttgg cggaaaactg ctgttccatc tgcgcgcggc tgatggtgga aagggggcca
    15181 tacatgccga atccggcatt gttaaagatc ccatacagac aattatcggt cagggcgatc
    15241 acttcgtcgg ctgcgcgatc aacactttct ggcgaatcca gatcgatcaa cacgccggta
    15301 aatcccatgc tgttcatacg ctcaacatca tccggtttcc ggcaacctgc cagcacatga
    15361 aaaccctggc gttttaattc gagcgcgctt tccaggccaa ttccactgga acatccggta
    15421 attaagaccg atttttgcat aactttacct gtcaggatct ccgttgcttt atgagtcatg
    15481 atttactaaa ggctgcaact gcttcgccat ccagtcggca ataaacggct gggcgtcgcg
    15541 gttgggatga ataccgtcat cctgcatcca ttgtggcttg aggtagacct cttccataaa
    15601 aaagggcagc agcggaacat caaactcttt ggcgagtttg gggtaaatgg cgctaaaggc
    15661 ttcattataa cggcgaccat agtttgcagg cagacgtatt tgcattaaca atggttcagc
    15721 gttggcggct ttgacatcct gcaaaatctg gcgcagcgtt tgctcggttt gctgtggctg
    15781 aaaaccacgc aaaccgtcat tgccgcccag ttcaaccagc acccaacgcg gctgatgctg
    15841 tttcagcaga gccggaaggc gcgccagtcc ttgttgcgag gtgtcgccgc tgatgctggc
    15901 attaactacc gacgttttac tctgccactt atcattcaac aaggcaggcc aggccgcgct
    15961 ggcagacatt cgatacccgg cgctcaggct atcacccaga atcaataacg tgtccgctgc
    16021 ggcggcacgg aaggttaaca gggccaggaa caggaagggc aaatgccagc ggaaaacatt
    16081 gttgaagttc atcatcttaa gaagtccgtc ggtcaggggg agcatgaact ctccatcctc
    16141 accggagttg agctggttgt caaacgtggc gagaccatcg cactggtggg cgagtcggga
    16201 tcgggtaagt caaccttgct ggcgatcctc gccgggcttg atgacggcag cagtggcgaa
    16261 gtgagtctgg tgggacaacc gctacataat atggacgaag aagcgcgggc aaagttgcgc
    16321 gcgaagcacg tcggctttgt ttttcagtca tttatgttaa ttcctaccct taacgcgctg
    16381 gaaaacgtcg agcttccggc tctgctgcgc ggtgagagta gcgcggaaag tcgtaacggg
    16441 gcgaaagcgt tgctcgaaca gttagggctg ggtaaacgtc tggatcatct tccggcacag
    16501 ctttccggcg gtgaacagca acgagtggcg ctggcacgag cctttaatgg tcgacctgat
    16561 gtgctgtttg ccgacgaacc caccggcaac cttgaccgcc agacgggcga taaaattgcc
    16621 gacctgctgt tttccctcaa ccgtgaacat ggcaccacgt tgattatggt gacccacgac
    16681 ctgcaactgg cggcacgctg cgaccgctgc ttacggctgg tgaacgggca gttgcaggag
    16741 gaagcatgat tgcacgttgg ttctggcgcg aatggcgttc gccgtcgcta ttaattgtct
    16801 ggctggcgct aagcctggcg gtggcctgcg tgctggcgct gggcaatatc agcgatcgca
    16861 tggagaaggg cttaagccag caaagccgtg agtttatggc gggcgatcgg gcgttgcgca
    16921 gttcacgcga agtgccgcaa gcgtggctgg aggaagcgca aaagcgcggc ctgaaagtcg
    16981 gcaagcagct gactttcgcc acaatgacct ttgcaggcga cacaccgcag ctggcgaacg
    17041 tcaaagcggt ggatgatatc tacccgatgt atggcgatct gcaaactaat ccccctggcc
    17101 tgaaaccgca ggcgggcagc gtattgctgg ccccacgcct gatggcactg cttaacctga
    17161 aaacgggcga caccattgac gtgggcgatg ccaccttgcg gattgccgga gaagtgattc
    17221 aggaaccgga ttccggtttt aaccccttcc agatggctcc gcgtctgatg atgaatctgg
    17281 cggatgtcga taaaaccgga gccgtgcagc cggggagtcg ggtcacctgg cgttataaat
    17341 tcggcggcaa cgagaaccag ctcgacggct atgataaatg gttgttacct cagcttaaac
    17401 ccgaacaacg ctggtacggt ctggaacagg acgaaggcgc gctggggcga tcgatggaac
    17461 gctcgcaaca gttcctgttg ctttcggcgc ttctgacctt gctgctggca gtggcagcgg
    17521 tggcggtagc gatgaatcat tactgtcgca gtcgctacga tctggtggcg atcctcaaaa
    17581 cgctaggggc agggcgagcg caactgcgta agctaatcgt cggtcagtgg ttgatggtgc
    17641 tgacgctttc agccgttacc ggtggggcca taggcctgtt gttcgaaaac gtgttgatgg
    17701 tgctgctcaa gcccgttctg cctgctgcac taccgccagc cagcctctgg ccgtggctgt
    17761 gggcgcttgg caccatgacg gtcatctcgc tgctggtggg gctacgacca taccgcttgt
    17821 tgctggcaac gcagccttta cgcgtattac gtaatgatgt ggtagcgaac gtctggccgc
    17881 tgaagtttta tctgccgatt gtcagtgtgg tggttgtgct gctgctcgcc ggattaatgg
    17941 gtggcagcat gctgctttgg gcggtgctgg cgggcgcggt agtactggct ttgctgtgcg
    18001 gtgtgctggg ctggatgcag ctgaatgtac ttcgccgcat gacgctgaaa tcgctacctc
    18061 tgcgcctggc ggttagccgc ctgttacgtc agccgtggtc aacgttaagc cagctttcgg
    18121 cattttcgct ctcctttatg ctgctggcgc tgttgctggt attgcgtggc gatctgctcg
    18181 accgctggca gcagcagcta ccgccagaaa gcccgaacta ctttttaatt aacatcgcca
    18241 cagaacaggt agcaccgcta aaagcgttcc tcgcggaaca tcatatagtc ccggaatcat
    18301 tttatccggt agtgcgggcg cggctgacgg cgattaacga taagccgaca gaaggtaatg
    18361 aagatgaggc gcttaatcgc gaactcaatc ttacctggca aaatacgcgg cccgatcata
    18421 atccgattgt cgccggtaac tggccgccaa aagccgatga agtgtcgatg gaagaggggc
    18481 tggcgaaacg cttaaacgtt gccctcggtg ataccgtgac ttttatgggc gatacccagg
    18541 agttccgcgc taaagtgacc agcctgcgca aagtggactg ggaaagtctg cggcctaatt
    18601 tctattttat tttcccggaa ggggcattag acgggcaacc gcagagctgg cttaccagtt
    18661 tccgctggga gaatggcaac ggcatgttga cgcaactcaa ccgccagttc ccgaccatta
    18721 gcctgttaga tattggcgcg attttaaaac aggtcggtca ggtgctggag caggtaagtc
    18781 gggcgctgga ggtgatggtg gtgctggtca ccgcctgcgg tatgttgctg ttgctggcac
    18841 aggtgcaggt gggaatgcgt cagcgtcatc aggagctggt ggtgtggcgc acactcggtg
    18901 cggggaaaaa actgctgcgt accacgttgt ggtgtgagtt cgccatgctt gggtttgttt
    18961 ctggcctggt agccgcaatt ggtgcggaaa cggcgctggc agtgttgcag gcgaaagtgt
    19021 ttgatttccc gtgggagcca gactggcgat tgtggattgt tctgccgtgc agcggtgcac
    19081 tgctgctgtc gcttttcggc ggctggctgg gtgcgcgact ggttaagggt aaggcgctgt
    19141 tcaggcagtt tgcggggtga tgacaggtgt tagattgcct gatgcgctac gcttatcagg
    19201 cctacgaaga gcgtgcaata tattgatttt gcaagattgt gtaggccgga taaggcgttt
    19261 acgccgcatc tggcatgaac agtgtgcatt ttggcaataa atatgaaacc aactgtcatg
    19321 tcggtttttt atatctgtat tctggtgcaa ccattctgtt ctcacgacaa tcgccgtttc
    19381 agaataccgc ccatcactgc gtcttaaccc attccgccac ttccgcccac tcaccgcgaa
    19441 agacaacttt ttccgctttt ttctcaagct gatagcgata catcgggtcg taatattctt
    19501 taagtaacgg caccagccag gccagatgac cgtcggtgct gtcggtggtg agttgcgttg
    19561 tcagcgccgt atccagctgg gcagcgagtt cgttatagcg ctgtaacccc agccgacgct
    19621 taatagccga aagtccgtga tgcaggtatt cgcaatattc ctgccagccc tgttcgtcgc
    19681 cgtacgcgtg ggtaaaatca tgatgcatac gcaagaaata ctcttcgttc aggcgctcaa
    19741 gacggatctc aaacggatct tctaccaccg caatcgccgc ctgagtcatt cgctcgcgca
    19801 ggcattccgg caggtgattc gaaccgatca tccggctttc gtcttccagc acccacaggc
    19861 gcaaatcctg acgggcgtcg gtttttagca tttcggcagc cagcaggttt tcaaaactcg
    19921 cctggctaag ttgtggttgt aaggtgcgac caaacgccga accgcgatga cgcgctaacc
    19981 cttccagatc aacaccgttc ggctgttgct gcactaacag cgttttaccg ctgccggtac
    20041 aaccgccaat cagcactatc ggtttttgtg ccagttcaat agtcgcctga atcgcggctt
    20101 ggcgcagtgc cttataaccg ccttccacca gcggataatc aatccccgct gcatacaacc
    20161 agctttgcac aatatgtgag cgctgaccgc cacgggcgca gcagagaata ccttgcggat
    20221 tttgcaggca cgctgcccgc caggcgtcca tgcgctgctg acgaatttca cccgccacca
    20281 gtttatgccc cagcgccagc gccgcgtctg agccttgctg tttataacaa gtgccaacgg
    20341 cggcgcgttc atcgttattc attaacggca ggttgatagc ggcgggcatt gcgccttgct
    20401 caaactcgat aggggcgcga acatcaataa tgggcgtatc agcaatcagc agggcacgat
    20461 agtcctgttc cgtgtgtctc tcttgcatag ttaaaagtga acctcaaatc agcttgcgcg
    20521 ctattttacg cgccaacgcg caaggaaact tgatttttaa ctgcgtgggt tgccgaaaat
    20581 ttctaaaaat ccgctgattt ccggcctgcg ctgggtaaac aaggtcacaa tatcttctac
    20641 cgctttgccg ctgccaaatt tgcgccatgc cagactcaat ggcgaaggag ggcgcatcgt
    20701 tgggattacc cggctgacca gttgttgatt atcgatcatt gactggcaaa gtgattttgg
    20761 caaaaaacca atgccaacac ccgccagatg ggcggcgatt ttcgtttcca tatcgggaac
    20821 aataatctct ttttgccccg gcaatcgcca ggcgacgcgt ttggttaagg ttcgggcgct
    20881 gtcttcaata ttgaccgccg gaaagcgccg caactgcgct tctgttagcg gctcttcaac
    20941 gttcgccagc ggatgatccg ccgccatgac aaagcgccat tgcaccgatc ctaagggatc
    21001 aagactaaag gtatttgcca gcgcctcagt tcccgtgacc ccgatagcca gcgaaaaacc
    21061 ttcgtacaat agcgagtccc agacgcccat atagatttgt cgggagatgt gaaactgggt
    21121 aaaggggtaa cgctcattca gccacgccag caactgggcg acggcctggg ggttatagag
    21181 caggttgttg atgacaatat tcacctagcg ttccacgcca tcattcacct gttgcagctc
    21241 gctgggcata ctttccagcc agctcagcca gtctctggcc tgggaaagta gatgctcgcc
    21301 agccgctgtc aacgtcacgc tgcgagtcgt gcggaaaaac agcgccactc cggtattctc
    21361 ttccagaagt ttaatgcgat aactgatcgt cgccgtggtt ttacataatc gttctgccgc
    21421 ttttgaaaaa cttcctgttt cagcaaccgc aatgaaagtc cgcaaggttt ctggatcgaa
    21481 catcttcagg tatccccttt taaatccgca agttgcgtga ttttcttatc ctctgattta
    21541 tcagtatttt tacatgataa ccctgttcaa tttgtggact aaatctagtt ttggaaaaat
    21601 attccaactt ttgtattgat gttgttctct taaggtttta gat
//
LOCUS       DAZPDU010000061        21507 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZPDU010000061 DAZPDU010000000
VERSION     DAZPDU010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21507
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.061"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            142..1839
                     /gene="ypdA"
                     /locus_tag="ACHURS_002087"
     CDS             142..1839
                     /gene="ypdA"
                     /locus_tag="ACHURS_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFR1237825.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSDGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            1854..2588
                     /gene="ypdB"
                     /locus_tag="ACHURS_002088"
     CDS             1854..2588
                     /gene="ypdB"
                     /locus_tag="ACHURS_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFR1237826.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            2601..3458
                     /locus_tag="ACHURS_002089"
     CDS             2601..3458
                     /locus_tag="ACHURS_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1237827.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(3461..5956)
                     /gene="ptsP"
                     /locus_tag="ACHURS_002090"
                     /pseudo
     CDS             complement(3461..5956)
                     /gene="ptsP"
                     /locus_tag="ACHURS_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            complement(5981..7018)
                     /gene="ypdE"
                     /locus_tag="ACHURS_002091"
     CDS             complement(5981..7018)
                     /gene="ypdE"
                     /locus_tag="ACHURS_002091"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFR1237828.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVSAEIGVPLQAD
                     MFSNGGTDGGAVHLTDTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(7018..8103)
                     /gene="ypdF"
                     /locus_tag="ACHURS_002092"
                     /pseudo
     CDS             complement(7018..8103)
                     /gene="ypdF"
                     /locus_tag="ACHURS_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(8118..9365)
                     /locus_tag="ACHURS_002093"
     CDS             complement(8118..9365)
                     /locus_tag="ACHURS_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFR1237829.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMLGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(9387..9713)
                     /locus_tag="ACHURS_002094"
     CDS             complement(9387..9713)
                     /locus_tag="ACHURS_002094"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFR1237830.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(9933..10898)
                     /gene="glk"
                     /locus_tag="ACHURS_002095"
     CDS             complement(9933..10898)
                     /gene="glk"
                     /locus_tag="ACHURS_002095"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFR1237831.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALTLGTFGGVYIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            11102..12358
                     /locus_tag="ACHURS_002096"
     CDS             11102..12358
                     /locus_tag="ACHURS_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFR1237832.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPIWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            12473..12799
                     /gene="ypeC"
                     /locus_tag="ACHURS_002097"
     CDS             12473..12799
                     /gene="ypeC"
                     /locus_tag="ACHURS_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFR1237833.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(12940..14178)
                     /locus_tag="ACHURS_002098"
     CDS             complement(12940..14178)
                     /locus_tag="ACHURS_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFR1237834.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            14514..15716
                     /gene="nupC"
                     /locus_tag="ACHURS_002099"
     CDS             14514..15716
                     /gene="nupC"
                     /locus_tag="ACHURS_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFR1237835.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLRFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(15766..17954)
                     /locus_tag="ACHURS_002100"
                     /pseudo
     CDS             complement(15766..17954)
                     /locus_tag="ACHURS_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
     gene            complement(18163..18238)
                     /locus_tag="ACHURS_002101"
     tRNA            complement(18163..18238)
                     /locus_tag="ACHURS_002101"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18203..18205),aa:Ala,seq:ggc)
     gene            complement(18278..18353)
                     /locus_tag="ACHURS_002102"
     tRNA            complement(18278..18353)
                     /locus_tag="ACHURS_002102"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18318..18320),aa:Ala,seq:ggc)
     gene            18574..18933
                     /locus_tag="ACHURS_002103"
     CDS             18574..18933
                     /locus_tag="ACHURS_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFR1237836.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            18935..19327
                     /locus_tag="ACHURS_002104"
     CDS             18935..19327
                     /locus_tag="ACHURS_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFR1237837.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQGKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(19379..20794)
                     /gene="gltX"
                     /locus_tag="ACHURS_002105"
     CDS             complement(19379..20794)
                     /gene="gltX"
                     /locus_tag="ACHURS_002105"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFR1237838.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            21053..21128
                     /locus_tag="ACHURS_002106"
     tRNA            21053..21128
                     /locus_tag="ACHURS_002106"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21086..21088,aa:Val,seq:tac)
     gene            21173..21248
                     /locus_tag="ACHURS_002107"
     tRNA            21173..21248
                     /locus_tag="ACHURS_002107"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21206..21208,aa:Val,seq:tac)
     gene            21295..21370
                     /locus_tag="ACHURS_002108"
     tRNA            21295..21370
                     /locus_tag="ACHURS_002108"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21328..21330,aa:Val,seq:tac)
     gene            21375..21450
                     /locus_tag="ACHURS_002109"
     tRNA            21375..21450
                     /locus_tag="ACHURS_002109"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21408..21410,aa:Lys,seq:ttt)
BASE COUNT         5312 a         5726 c         5342 g         5127 t
ORIGIN      
        1 attatcaaga gatacattgc taatacacca tttatctttc aatccccttt taaatttatg
       61 gtttttcctc aaaaacggcc tgctggtcat cagccttcag gttacctatc atagaggttt
      121 aatccttatt cagagtcacc cgtgcacgaa atattcaaca tgctgctggc ggtcttcgat
      181 cgggcagcgt taatgcttat ctgcctgttc tttctcatcc gtatccgcct gtttcgcgaa
      241 ctgttgcaca agtcggcgca ctccccaaaa gaattgctcg ccgttaccgc cattttctcg
      301 ctgttcgccc tgttcagcac ctggtccggc gttcccgtag aaggctcgct ggtgaacgta
      361 cgtattatcg cggtgatgtc cgacgggatt ctgtttggcc cgtgggtagg catcattacc
      421 ggcgtgattg cgggtattca ccggtattta attgatatcg gcggcgtgac ggcgatcccc
      481 tgctttatca ccagcattct ggcgggttgt atatcaggct ggataaacct gaaaatcccc
      541 aaagcacagc gctggcgcgt cggtattctc ggcggcatgt tgtgtgagac gttgaccatg
      601 attctggtaa ttgtctgggc accaactacc gcgttgggga tcgatatcgt ctctaaaatc
      661 ggcattccaa tgatcctcgg tagcgtctgt atcggcttta ttgtgcttct ggtgcaaagc
      721 gttgagggcg aaaaagaggc cagcgccgcg cggcaggcca agctggcgct ggatatcgcc
      781 aacaaaacgc taccgctgtt tcgccatgtc aatagcgagt cattacgcaa ggtctgcgaa
      841 attatccgcg atgacattca cgccgatgcg gtggcgatta ctaataccga tcatgtgctg
      901 gcctatgttg gcgtgggtga acataactat cagaatggcg atgacttcat tagcccgact
      961 acccgtcagg cgatgaatta cggaaaaatc atcattaaaa acaatgatga agcccaccgc
     1021 acaccagaga ttcattccat gctggtgatc ccattgtggg agaaaggggt cgtgaccgga
     1081 acgctgaaaa tttactactg ccacgcgcat cagatcacct cgtcattaca ggaaatggcg
     1141 gtcggtctgt cgcaaatcat ctccacgcaa ctggaggttt cacgcgccga gcagctacgt
     1201 gaaatggcaa ataaggcaga gcttcgcgcc ctgcaaagca aaattaatcc ccattttctg
     1261 tttaacgctc tgaacgctat ttcatcgtca atccgtctga atccggatac cgctcgccag
     1321 ttgatcttta atctgtcgcg ttatctgcgc tataacattg aattaaaaga cgatgagcaa
     1381 atcgatatca aaaaagagct gtatcaaatt aaagactata ttgccattga gcaggcccgc
     1441 tttggtgaca agctgacggt tatctatgat attgatgaag aggtgaattg ctgcattccc
     1501 agcctgctga tccagccgtt ggtggagaac gccattgtcc acggtattca gccttgcaaa
     1561 ggtaaaggcg ttgtcaccat cagcgttgca gagtgcggaa atcgggtacg cattgcggtg
     1621 cgagataccg ggcacggcat cgatccaaag gtgattgagc gggtcgaagc gaatgaaatg
     1681 ccgggcaata aaattggcct gctgaatgtc catcatcgcg tgaagttatt gtatggcgag
     1741 gggctgcata tccgccgcct ggagccgggg acggaaattg cgttttacat tcctaaccaa
     1801 cgcaccccag tcgcctcaca ggctacgtta ttgctttgag ccggagtgat attgtgaaag
     1861 tcatcattgt tgaagacgaa ttcctggcac aacaggaact gagctggcta attaaagagc
     1921 acagccagat ggagattgtc ggcacctttg acgacggtct ggacgtgttg aagtttttgc
     1981 agcataaccg cgtcgacgcc atttttctgg atatcaatat tccgtcgctg gatggcgtgt
     2041 tgctggcgca aaacatcagc cagttcgccc ataaaccgtt tattgtgttc atcaccgcgt
     2101 ggaaagaaca tgcggtagaa gcgtttgaac tggaggcgtt tgactacatt ctcaaaccgt
     2161 atcaggagtc acgtattacc gggatgctgc aaaaactgga agcggcctgg caacaacagc
     2221 agaccagcag tacgcctgcc gcgacggtaa cgcgtgagaa tgacaccatt aatctggtga
     2281 aagatgagcg aataatcgtc acgccaatta acgatatcta ttacgccgaa gcgcacgaga
     2341 aaatgacctt tgtctatacg cggcgtgaat cctacgtaat gccgatgaac attaccgaat
     2401 tttgcagcaa actgccgccg tcgcattttt tccgctgcca tcgctcattt tgtgtcaatc
     2461 tgaacaaaat acgcgaaatc gaaccgtggt ttaataacac ctacattctg cgactgaaag
     2521 atctggattt tgaagtgccg gtcagccgca gcaaagtgaa agaatttcgc cagttaatgc
     2581 atctttaatc gggaactttc atgaaagcac caggcttgcc cgccgatcaa caatttttcg
     2641 ccgatctgtt cagcggcctg gtgcttaacc cgcaactact ggggcgcgtc tggtttgcca
     2701 gccagcctgc ctcgttaccg gtgggcagtt tatgtattga ttttccccgt ctggatatcg
     2761 tgctgcgcgg cgaatacggc aacctgctgg aagcaaagca gcagcgaatg gtggaaggag
     2821 aaatgctgtt tattccggca cgcgcggcta atttaccgat caacaacaaa ccggtgatgc
     2881 tgttaagcct ggtgttcgca ccgacctggc ttgggttatc gttttacgat agccgcacca
     2941 cgtcgttgtt gcatcctgct cgccagatcc agcttcccag cctgcaacgc ggtgaaggtg
     3001 aagcgatgct taccgccctc acccatctta gccgttcgcc gctggagcag aatatcattc
     3061 agccactggt gttaagtttg ctgcatcttt gccgcaacgt ggtgaatatg ccgccgggta
     3121 attcgcaacc gcgcggcgat tttctctatc acagcatttg taactgggtt caggataatt
     3181 atgcccagcc gctcacccgc gagagcgtgg cgcagttttt taatatcacg cccaatcatc
     3241 tgtcaaaact gtttgctcag catggaacga tgcgttttat cgagtatgtg cgttgggtgc
     3301 gaatggcgaa ggcgaggatg attttgcaga aatatcatct gtcaattcat gaagtggcac
     3361 agcgttgcgg ttttccggat agcgactatt tttgtcgcgt tttccggcgt cagtttggtc
     3421 tgacgccggg agagtacagc gcccgttttc agggctaacg tcagaaggtt aattctgttt
     3481 ccagcagcgt caggatactt tgtgcatctt gcgcggcaaa caacgactgg cggaagtttt
     3541 tattcaccag tttacgcgcc agctgcgaga agactttcac atgattcatc ccttcgttag
     3601 cacccagcgt cagcatgatc accagttcaa cttcgcccat ttctgactgc cagtcaaccg
     3661 gtttcgccag ccgggcaatg ctgatactgg aatgacggat ccactgagat ttggtgtgcg
     3721 ggatcgctac gccaaaacca acgccggtgg taacaatctc ttcccgctgc cagacatctt
     3781 cttccagctc aaacggatgt tcagtgcgcc cgttaacgcc gaggttgccg cacaggaact
     3841 ggatcgcctg ctctttattg ctaaaatcct gatcaacaaa gatattctcc agcgccagca
     3901 gtgggcgaac gtcttcttcc ggcgtaaagg cggtgagtaa cgcttcaatt tcctgcgcac
     3961 tgcggcattc acatgcctga cgcgccagtt cccgacacgc ctcgctatcc agttgacgaa
     4021 gctggctttt caccgccgga atacgcgggc tactcatact cagctcatcc aggcccagcc
     4081 caagcagtag cggcagataa cggctttcac cgcccagttc accgcaaatg cctacccatt
     4141 tgccccgctg atgcgcagtg gtaactattt gctgcaacat gcgcaggaac gatggcgtaa
     4201 tcgggttata tagcggcgat acgcgcgggt tattacgatc gaccgcatac agatactggg
     4261 tcatatcgtt ggagccgata ctgaagaaat cgacctcatc ggagaagtgg tcgatgatgt
     4321 agcacaccga cggcacttcc accatgatcc caagcgtaat cgtctctgca tgacgcaggc
     4381 catcgcgctt aagctcaacg atcgcttttt ggatctcgcc tttcacccat aagatctgat
     4441 cgaggctgtg aaccatcggg atcatcaact gggcgttgcc gaaactggcg gcgcgcaaaa
     4501 tggcccgcag ttgagtgcgg aacaggccag caaattccgg gtaaatacgt accgcgcgat
     4561 agccgaggaa cgggttctct tcctggggaa tattcagata aggaatgctt ttatcgccgc
     4621 cgatgtccat cgtgcggaag ataatcggct tgtcgcccgc cgccagtagc acctgctggt
     4681 aggcttcaaa ttgctcctgc tcgtccggcg cgctgtcgcg atccatatag agcatttcgg
     4741 tacggaacag cccgacacct tccgcgccgt tggcaaacgc gcctggcgct tccagagcgg
     4801 tgccgatatt cgccgcaata tcaatacgct tgttatcacg ggaataggcc agctgcgcgg
     4861 cagcctgcgc ctgttgtttt tggcgtttat ccgccagcgt ctgcgcgacc tgataataac
     4921 cgctcaccgc gtcattcggg ttaatcgcca gcacgccgca ctgggcgtca agcactgcag
     4981 gttgcccggc ataacgggca atcgcatcca gcggcaagcc actcagtacc gggatcgccg
     5041 aggcacgggc cagaatcagt gtatgcgagg tgcgcccggt tttctccaga atcatgcccg
     5101 caagattttt caaatcaagg ctcaaaaact ggcttggggt taaatcttca gccaccagaa
     5161 tggtcggttt ttccagcacc agattgttgc gcggcttcag ttccggctag gtgatatgca
     5221 gcaactgttc gctgatatcg cgaatgtcgc tgacccgctc gcgcagataa tcgctggcag
     5281 aagcagatag tttggcgcaa acctgctcca tattgctgat gatcgccgcc cccagtccct
     5341 gatgctgttc tgtcatcagg cgacggatat tgcctgcaaa ttcatcatcc tgaatcagcg
     5401 acaaatgagc gctgaggata gttttgcttt cgccgtcacg ctcacgcaat tgctggttca
     5461 gttgctcggc aagcgttgcc aggctgtgct ccagccgggt ggaatcttgc gcactggcgg
     5521 ggattgcccg ataactgtcg aggctgtcgc tttgtaacag ggtcagcgta cccacgccga
     5581 cgccgcttgc cagcacattg ccgtacagta aatccgggtt aaggcggctt aatgaacgcg
     5641 gcagcggatg cgccgtcagt tctgcctgcg tcggctgaac gctgtcgctg tcgataaagc
     5701 gcaccaggat gtactcttcc agcacgcgcc gcgcctgctc ttcatcgctg ccgctaatgt
     5761 tcaggctgca actgtcatta aataaggtgc cagttccaat caacgccagc gagcttttag
     5821 catcggcctt tgcgttctgg cgatggttga taaaggtgat ttcgctttgc cactggctgc
     5881 actgttcttt aagttcccac gccggacgag catgtagacc gttaggcaaa ggacagagaa
     5941 attgaatcgt taacataacc gctccgggtt aatcaggaga tcatctgaaa tccgtcagtt
     6001 gaacaaccgt ctcacgcgta agacgttgaa taagggcaga taaaagttgc tccatctgca
     6061 aaatgtcgcg gcaatcggca atcgatgccg cgcaatgtcc gtggcgggtg gcaggcccca
     6121 ttaccactgt gggtacgcca gtatcggtta agtgcaccgc tccaccgtcc gtgccgccgt
     6181 tactgaacat atccgcctgc aacggaacgc caatttctgc cgacacggtt tcaatccacg
     6241 cggtaagttt tggcggcgca atcagcgatt tatcgcttaa caccagcatc ggcccgttac
     6301 caatctgacg atggttagcc gcgccataat caaagttttt cgcccagcag gcggtatcaa
     6361 gcacaatggc gacgtccggc gacaccgcgc gggtagcagt ttgcccgccg cgtaatccca
     6421 cctcttcgct ggaacttgcc accagccaca cttccgcagg cagttcggcg tcgtgcagct
     6481 cacgtagtaa cgtcaccagc agatagcaac cgaggcggtc atcaaaggct ttccccatca
     6541 cccgctggtg agggagaacc tgaaaagttg tatcaaaggt gacgcgatcg cctggacgaa
     6601 ttcccgcctg catcacttcg tcatacgagc gcgcaccaat gtccacgcgc atggcgctga
     6661 cgtcattccc ctgccggtcg ccgtcaagca ggcctggaat tttgcactct tcacgggtgg
     6721 tgatgcgcac cggctgcagc tggcgggcag ccatgcgcac gttgccaacc ggcagcacat
     6781 caattgcccc ttcgcgggag atgctgcgca ccataaatcc cacttcgtcc atatgcgcac
     6841 agatcatcac cttcggacct gtcgattcat tgaggcggat cagcaccgat cccagaccat
     6901 caaatcgtac ttctttttgc aggcgatccg cttcttccag taggatctgc cgcacttcct
     6961 gttccgagga ggcgatcgca tctgcctcgc tcaacgcttt taatagcgat aaatccatta
     7021 tgcctctccc gtgagcaaca ctgttttcgg catggcgtag agcacttctg cgccttgcgg
     7081 ggtaaccagc acaacatctt cgatgcgcac gcccccttgc cctggcaaat aaatccccgg
     7141 ctccacggtc agtaacatgc ctggctgtag cgtcgtggta tcccgcggtg aaaagcgtgg
     7201 atcttcgtgt acttcaatac cgatagcatg accggtgttg tgaccaaaat agtcgccaaa
     7261 tcctgcctcg gtaatcaccc ggcgcgcggc gtcgtcaacc tgttagcagc gtacgccagg
     7321 gcgaatcgcg gagattgccg ccagctgtgc ttgcaggaca atctgataga cgttaaacag
     7381 cgggtgagat tcggcaatta ccccttcgcc attcaccagc aaggtgcgcg tcatatcaga
     7441 gcagtacccc tgatagagcg caccaaaatc aagagtgaca aactcgcctg ctgcaacaat
     7501 cttgtcgctg gctttgccgt gcggcagcgc cccacgccag ccgctggcga caatggtgtc
     7561 aaaagaggct ttttctgcgc cctgctggcg cataaaccac tccagttcag cggctatctc
     7621 gcgctcgctc atccccgcct gaataaagcg gcgaatatgc tccgctccgc gatcggcaat
     7681 cccacaggcg aggcggatta tctccacctc ctctggcgtt ttgatttgcc gcagcacatc
     7741 cggcgtggcg ctaaccagtt tcgcattgag ttcagacttc cagcggtgcg cggtttccca
     7801 actcacctgc tggccttcaa aaccgagcgt ttgcaactgc tcatcggcaa tgatttgatt
     7861 gacgatagtg gtaagcgtgt tcgtcgcgtc aagcaaatgc agctggtagc cttgcgcacg
     7921 ggcttccacc tcaacgaaat agcgcgaatc taccagaatg tgcgcacttt cacggctaat
     7981 aaccacatag cctgatccgg tggagatccc cagatgcggc tgtttgttct gccgtgagga
     8041 gagaagcact gcatccagtt gttgcgcctt aagccagtcg cgcagcgaag cgagtaatgt
     8101 catctggatt ttccttctta caggctatcg attaacaatt tacctttacg gaacatcatc
     8161 agacgcagga acaccaccat cagcgcagtt atgactgccc ccagcgcgat ccccgccata
     8221 tagacgccca ggttagtgac cagcggccat gcccaaatag cggattccgg gaaccattgc
     8281 actgcgccca gccagacagc ggcggttgag ccgacaatcg cgccgaccat atacgacgga
     8341 atggcggtga tggggctttc cagcgcaaac ggaatcgcac cctcactaat ccccataaag
     8401 gcaaggaaca tcgcggtctt accctgcgga taaagttgag cgttgaacag gcgtttgccg
     8461 gttaaacgac ggtcgataat ggtcgccaga cccagaccta tcggcggaat aacgatagcg
     8521 atagaacgtg cagtgaccgg taatacgtgg tcagtggtaa aactgaaggc aacaaaacct
     8581 gcggctttgt tgatcgggcc accaaggtcg attgcggtcg ccgcggcgat ccccattgcg
     8641 tacatcagcg cgcccttctc acctgcggca gtcagcacgg tacggatacc gccgttgatc
     8701 cagccgccaa agggggtaat gacgtagtac atcgccagca tgacaaaaat ggcggaaaga
     8761 atcggtagca aaaatgtggt tttgaacgct aacaggaaat ccggtaactg gattttctgg
     8821 ttcatccact tcacaaggta acccgcgacg atagaaataa tcagcgcacc aatgaaggtg
     8881 gacggcaccg gcgaagaggt cgcccattgc atagtgctgg gatcgaagtt cagcagttgc
     8941 gtcggctggg tagacatcaa gccgccgata aaaccagccg ggaacgccag tttgccaccg
     9001 atagagttcg ccacaaaagc agcaaacatc ggaatggcaa aaccaaacaa cacgccgccg
     9061 aaggactgcg acagccaggc aaatttcagt aaggagaggt cgaaaccgga gaatttcccg
     9121 ctattaagag catccatgat gccgatctca gcaggaattt taagccagct ataagcaatc
     9181 agctggctga aagcgaggat cacaccgccc ataattaagg tcggcaccat gcgtgaaatc
     9241 cctaacatca catgctgcgg gagttcaccc cagaagctgc ttttagaggc ctgcggattc
     9301 ttaactgccg ctgccgcacc ggatgcgcca ggcacaacgg ttgcactgcg ttttttaatg
     9361 gccataacaa ttatccctgt cgaatattac tgttgttcag aggcaatcat ctcttcgatt
     9421 tctttgatga tgcccgcagc gtttttaatt gcgtcctgca aagtgatttc ataaacgtcg
     9481 cgtgattcga aacgctcatt atcttccggg gtaactgcca cggagtggat gatgatggtt
     9541 gcttcggcga tatcctgcgc cgtcaggcga ttctggatac cgtccgcgcc ctgggtttca
     9601 attttcactt cataacctgc ttctaccgcc gcttcttcca gcgcctgagc ggccataaag
     9661 gtgtgagcca ggcccattgg gcaggcacat aaggcaatta gtttcttact catcgttaat
     9721 tcctcgttca gtgatagttg acgagtgaaa ttatgaacgt gcaaaacaaa tcgccgttac
     9781 cagacaaata atgcatttac tggagaatta atcccgctgt acggaagtgt gacgcaggtc
     9841 ggccttgtga gggggacaca aagaaaaatt gaattaactg tatgatttaa aagattatcg
     9901 ggagagttac ctcccgatat aacagaaagg atttacagaa tgtgccctaa ggtctggcgt
     9961 aaatgtgcac cggaaccgag aagacccgga ttgtcatgga cgatgagata caccggaata
    10021 tcatggacat attctttaaa gcgcccttta tcttcaaatg cggcacggaa accagaggct
    10081 ttgaagaact caaggaagcg cggcacgata ccgccagcaa tataaacccc accaaaggtt
    10141 cccagcgtta atgccagatt gccgccaaaa cggcccataa tgacgcaaaa cagcgacaac
    10201 gcacggcggc aatcggtgca gctgtcagcc agcgcgcgtt cggtaatatc ttttggcttg
    10261 agattttctg gcaggcggtt gtcggcttta acaattgcgc gatacaaatt caccagccca
    10321 ggaccagaaa gcacgcgttc cgccgaaaca tgaccaattt ccgcacgcaa tatttcgagg
    10381 ataatggcct cttcttcact attcggcgca aaatcaacgt gaccgccttc gcccggcaag
    10441 cttacccaac gcttatcgac atggaccaga tgcgcaaccc caagccctgt tccggcaccg
    10501 taaaccgcaa taggtttacc ttcgaccggt tctgcgccac caaactgaat cagatgctct
    10561 tttttcagca tcgggatcgc catcgataca gcggtaaaat cgttaataat ttccagatgg
    10621 ctaaaaccga gattcttttt catttcggca attgagaacg cccaggtatg gttggtcatt
    10681 gccacccagt caccggtaat tgggcaagcg atggcaatac agccgtcttt cacctcgacc
    10741 ttatgttctt caagataaac gcgaatgacc gcttcgaggc tggggtaatc aagccctgaa
    10801 taggtcttag cctgcgagat ttcaccactg gcaatatcac acagagcaag acgtgcgttg
    10861 gtgccgccca catcaccgac taatgcatac tttgtcattc ttcaactgct ccgctaaagt
    10921 caaaataatt ctttctcaca ctgtaaatac ctgggggcat aacaacaacg ccggaaaggc
    10981 aggctccctg taaatatcga tctgggtcac acatttactt tatcgtttca gcaccaattg
    11041 cagcgatgcc tttttgcaag ctgggcaaac taagtatctg accccgcata aggaatagaa
    11101 catgctccat ccgcgagcca gaaccatgtt gttattatcg ctccccgccg tggcaattgg
    11161 gattgcgtcc agtcttattc tgattgtggt gatgaaaatc gcctcggtat tacagaattt
    11221 gctctggcaa cgactgccgg gaactctggg gatagcccag gattcaccta tctggatcat
    11281 cggtgtatta acgctaacgg gtattgcggt ggggttggtt atccgtttca gccagggtca
    11341 tgccggacca gaccccgcct gtgaaccgct gatcggcgca ccagttccgc cctctgcgct
    11401 accaggactt atcgtagcat taattctcgg tcttgctggc ggcgtcagcc tggggccgga
    11461 acatccgatc atgaccgtca atatcgccct tgccgtcgcc attggcgctc gtctgttacc
    11521 gcgcgtcaac cgaatggagt ggactatttt agcctctgcc ggaaccatcg gcgcgctgtt
    11581 tggtacacct gttgcggcgg cgttgatatt ttcgcaaacc ttaaatggca gtagtgaagt
    11641 tccgctatgg gatcgtcttt ttgcgccgtt aatggcggca gcagctggtg cacttactac
    11701 cggattattt ttccatcccc atttttcact gcccattgcg cattacggac agatggaaat
    11761 gaccgatatt ctcagcggtg caattgtcgc ggcgattgcc atcgcagcag ggatggtcgc
    11821 cgtatggtgc ttaccacggt tgcacgcgat gatgaatcaa atgaaaaatc cggtgctcgt
    11881 gctgggtatt ggcggattta ttctcggaat tctgggggtt attggtggac cagtttcgct
    11941 gtttaaaggg ctggatgaga tgcagcagat ggtggcaaat caggctttca gcaccagcga
    12001 ttactttttg ctggcggtaa ttaaacttgc cgccctggtc gttgctgccg ccagtggctt
    12061 tcgcggtggg cgaatcttcc cggcagtgtt tgtcggcgtg gcattagggt tgatgctgca
    12121 tgagcacgtt cccgccgttc cagcggcaat aaccgtttcc tgcgctattc tcggcatcgt
    12181 gctggtggta acacgcgatg gctggttaag tctttttatg gcggcagtcg ttgtacccaa
    12241 taccacattg ctaccgctgc tctgtatcgt catgcttccg gcatggctgt tattagcagg
    12301 taagccgatg atgatggtca atcgtccgaa gcaacagcca ccccacgata atgtttagca
    12361 aaaaatgctc tcctttatga tttaagagtt atggcgatga tacggggctt tacctccccg
    12421 taatattgcg ttaacaggcc gctgacggct tcgatcgtga aggagaataa caatgttcag
    12481 gtcactgttt ctggcggccg ccctgatggc atttaccccg cttgcagcaa atgcaggtga
    12541 aatcacccta ctgccatcga tcaaattaca aattggcgat cgcgatcatt acggtaatta
    12601 ctgggacggt ggtcactggc gagatcgtga ttactggcat cgcaattatg aatggcgcaa
    12661 aaaccgctgg tggcgtcatg ataatggcta tcatcgtggc tgggataagc gtaaagcata
    12721 tgagcgtggc tatcgtgaag gctggcgcga tcgtgacgat catcgcggaa aaggccgcgg
    12781 acatgggcac cgccattaaa cgcgtcctcc aatggagcac aatgcctgat gcgacgctag
    12841 cgcgtcttat caggcctaca aaccggctct ttaacgcgta gggcggataa ggcgtttacg
    12901 ccgcatccgc caatagtgcc agatgcgacg ctcattcaac tacaatccca gcgccgtccc
    12961 caccaacaac cagatattca gcgccacgac cagcactaca atcacccagc ctgtctgttt
    13021 tacgcgtttg ctgttcacca gatcgcccat caacttgctg tcactggtga aaatcagcag
    13081 tggaaccagc gccagggcga taccaaaact taacagcacc tgactcataa ccagaatccg
    13141 tgtcggatct aatcccatca gaatgacaat aaatgacggc aacatggtga ctgtacgacg
    13201 cacccacagc gggatatgga agcgaatgaa tccctgcatc accacctgcc ccgccagtgt
    13261 ccccaccacc gttgaggaca gtccggcagc aaccagactt aacccaaaga ccgttgccgc
    13321 agcatggctt aacagcggtt gcagcgtcag ataagcctca tcaagatcgg caacaccagt
    13381 atgaccagaa aagtggaacg ccgccgcagc tgtagccatc atcgccagat tgacaaaacc
    13441 ggcaatcgtc atggcgatag ccacatccca tttggtggcg gaataacgtt gttgacgcga
    13501 accgccatgt aaatgctgag tgagcgaaga gtgcaaataa atcacatgcg gcataatcgt
    13561 cgcccctaac acgcctgctg ccaggaagac cgcctccgaa gtaggtaaac tcgggatcac
    13621 cattccttta cccagctgcg ccaggttagg ctgggagaaa atcaactcga caatgtaagc
    13681 cgcggcaaca aacaacagta acccgccaat cactttctcc agtggttttt gcccgcgacg
    13741 ttgcagcatt aaaatcagga aagtcgcgat ccccgtcagc accgcgccct gcaacaacga
    13801 gacaccaaga atgagtttaa aaccgatcgc cgcaccaata aattccgcca ggtcggttgc
    13861 catcgcaata atttctgcct gaacccaata gaaccacact acgggacgcg gatagtgatc
    13921 acgaatctgc tccgccagat tttttccggt ggcaatccct agtttggcag agaggatctg
    13981 aatcagcatc gccatcaggt tggcccaaac gacaacccac agtagctgat agccgaagct
    14041 ggcacccgcc tgaatattgg tcgcaaagtt accgggatcg atataaccaa tcgccgcaat
    14101 gaacgcaggt cccattaatg cgagcctcat cttgcgcgcc gcccgtccgc tgctactctc
    14161 aacgcgatag ttcgtcatct tgtgcctcta aaacatagcc tttgctatgt ttcatgctat
    14221 gataaacgag aatgattatc aaattcattt aaatggattg tggtgatttc tctgatagac
    14281 caggattatg actacgaaaa gattgatgac ttgaatgtta tggttatgtt taatgttagc
    14341 acatttacat aactttcagc ttccatacac aacatagcag aaatgtatga cagatcacta
    14401 tttttgaagc ctgtcacagg acgtcattat agtgtgtgtc agatctcgtt ttccttaacc
    14461 atgttacata gaatgtgcac ggaaatttaa cctgcctcat atttggagca aatatggacc
    14521 gcgtccttca ttttgtactg gcacttgccg ttgttgcgat tctcgcactg ctggtaagca
    14581 gcgaccgcaa aaaaattcgt atccgttatg ttattcaact gcttgttatc gaagtgttac
    14641 tggcgtggtt cttcctgaac tccgacgttg gtttaggctt cgtgaaaggc ttctccgaaa
    14701 tgttcgaaaa actgctcaga tttgccaacg aagggactaa cttcgtcttt ggtagcatga
    14761 atgatcaagg cctggcattc ttcttcctga aagtgctgtg cccaatcgtc tttatctctg
    14821 cactgatcgg tattctccag cacattcgcg tgttgccggt gattatccgc gcaattggtt
    14881 tcctgctctc caaagtcaac ggtatgggca aactggaatc ctttaacgcc gtcagctccc
    14941 tgattctggg tcagtctgaa aactttattg cctataaaga tatcctcggc aaaatctccc
    15001 gtaatcgtat gtacaccatg gctgccacgg caatgtccac cgtgtcgatg tccatcgttg
    15061 gtgcatacat gaccatgctg gaaccgaaat acgtcgttgc tgcgctggta ctgaacatgt
    15121 tcagcacctt tatcgtgctg tcgctgatca atccttaccg tgttgatgcc agtgaagaaa
    15181 acatccagat gtccaacctg cacgaaggtc agagcttctt cgaaatgctg ggtgaataca
    15241 ttctggcagg tttcaaagtt gccattatcg ttgccgcgat gctgatcggc tttatcgccc
    15301 tgatcgccgc gctgaacgca ctgtttgcca ccgttactgg ctggtttggc tacagcatct
    15361 ccttccaggg catcctgggc tacatcttct atccgattgc atgggtgatg ggtgttcctt
    15421 ccagtgaagc actgcaagtg ggcagtatca tggcgaccaa actggtttcc aacgagttcg
    15481 ttgcgatgat ggatctgcag aaaattgctt ccacgctctc tccgcgtgct gaaggcatca
    15541 tctctgtgtt cctggtttcc ttcgctaact tctcttcaat cgggattatc gcaggtgcag
    15601 ttaaaggcct gaatgaagag caaggtaacg tggtttctcg cttcggtctg aaactggttt
    15661 acggctctac cctggtgagt gtgctgtctg cgtcaatcgc agcactggtg ctgtaaaacc
    15721 atacataaaa aagccgggga tcattccccg gcttttttac gcctgttaat cagctaatgg
    15781 ctgcgggcga ccaatcaaat acccttgcag atattgcacc ccgagcttat gcaatagcgc
    15841 ctgctgctgt tgcgtctcga caaactccgc gaccacactc aatgacttcg ctttcgccag
    15901 atcggtaatt gatctcacaa tcatcgcatc cagcgtattc gtgacaatat ctttcacaaa
    15961 gacgccatca attttgatga tatcagcctg caaacgcttt aaccgttcgt agttggcata
    16021 tccggtgcca aagtcatcaa tcgcaatccg gaaaccaaac ttatgcagct gctcgatgtt
    16081 gtacatgctg ctttctgcgt tagaaaacgc ctgctcctcg gtgatctcaa gaatgaccgc
    16141 ctgcggggag atgtgataac gcttaaacag acgaataatc cgcccggcaa tattcttttg
    16201 cagcagcgtg agcggcatta aattgactga aaagcgcggt ccttttttgt cgcaagggtg
    16261 tgttgccagc cacttcaaca aggattccag cacttgcaaa tcaaaacgcg cgctaaggtt
    16321 aaactgagca ataaggggca gaaacttatc cggggtcata atgccgccgt catatttcag
    16381 tcgcgcgagg atctcatcat aaccttcacc ctctttgttg cgaattggct gggcgtagag
    16441 cagcaaatca ccctgatcta acgccgtgcg aatggtattc agcaatagca cctgtttagt
    16501 ggtctgcccg gaaaccatct cctctctgct atccagcgcc agcacatgat gatgtgcgca
    16561 ggattgctcc gccagccagc ttaactgccc caacaagggt tgcagggttt cctgatttcc
    16621 atcaaaacgc ccccaggcag caccatagcc catatccagc ccggtattgt tccagtgaat
    16681 ttgccgacta ttcaggatgt taaccatatg ctggagtcgc ccttccgttt ccggcccgct
    16741 cagcactaac agcagttcac tacccggcaa ttgatataac ttttcgtttt cctgcatcaa
    16801 cggcagcagc gtacggtaaa ttgatcggat acagtgaacg cgcatcatta agccgtaatg
    16861 acgactcata aactcaagat tatcaatgcg caggcagcaa aactcttgcc cgcctcttgc
    16921 tccggcgctt gttccaacgc acggaagttg ggcaggagcg ttaacggatc ggtcagcgcc
    16981 tgcaaatgcc agcggcgatt aagccattca ctgcgatgat aaatgcgcac catatagagc
    17041 aggcaaacgc taaaggaaat cagcaccgca agaataaatg ccagcgaata ttcggtttcc
    17101 accccttgca aaaagttctg gttgtaattc agaaggcaaa gcgttgaaac agcccaggtg
    17161 agatttaaaa acggatagcg aagcttaccg accccgaggg taaagatgat gaagaaaacg
    17221 ggtaccaggt aaccggcaat aaagtcgttt tcataaggtg tgcacaacag aagtagaagc
    17281 acgcttaacg ctgccagcca gcttaaggta aatgcgcgtt tctctttgcc caacgacggc
    17341 gcgatatccc tgcgccacaa tatctgcgca aagtggggac ttacaatcat gcgagtgaga
    17401 tagtagaaaa gcatgttgta aataagcaca gcggtgaaaa ggcttagcaa atcaacgacc
    17461 gtgaaaatgg catccgcatc gccgaaaaag gtggatatct ttagcgggaa atcaaagaag
    17521 ctccccacaa gatacatgct gcatttgatg ccgatcggcg tcaccaaacc aagccagaac
    17581 agacgttgcc agacatatcg gctggtcaat ccataacgcc aacgcgtccc cagctgccag
    17641 cgtaatattg cgcaggcaca caacacgaca aacgtctggc taccgagcat gaccgcagtc
    17701 tgtaaaaaag agagatgaaa gttccacaga ttggtgcaaa acatcccgca taaaacggga
    17761 acgacaccgc gccagccaaa gataaacaag attgacagca ttacgcacag tggcatccac
    17821 gccagaaaga tataactgga atggataatg gcaagtggcg aaataaaacg ggatagctga
    17881 ataagtacca cggtgagcgt aaacgctagt gtgaatatct tgatattttt tatcaggtta
    17941 tgctccacaa acatgagtac actactctca ctgatcgggg ttttcaggca cacaaataat
    18001 aagtttattc gcatcgcgaa gcaaacaggg ggccagcctg ttgccgattt ttcatccaat
    18061 gatgctttca ccaggcggca ctaacccgga cgtagactgt attacaaaag cggcaaaaag
    18121 cagagacaaa aaacccccgc tttgcagcga gggttggaaa tttggtggag ctaagcggga
    18181 tcgaaccgct gacctcttgc atgccatgca agcgctctcc cagctgagct atagccccac
    18241 gatgcgttta cgtaccaaat ttgctgggtg caaaatttgg tggagctaag cgggatcgaa
    18301 ccgctgacct cttgcatgcc atgcaagcgc tctcccagct gagctatagc cccgtcacgt
    18361 aaagcttgtc gagttgacgg gcggcatcat atgaattccg cccgaatgtg tcaacggcaa
    18421 attgcaacgt gtagtttcaa tcgctgaaaa atcaggcaaa tgaacaattt tggaaaaccg
    18481 ctcgcattca gtagttattc atgtcacggt ttcctgtaaa gtggtgttat aaaatgaact
    18541 actaatagac ccacatacat tcagggaatt gttatgttca aggagaggat gacaccagat
    18601 gaacttgcca gactgaccgg ctatagccgc cagaccatta ataaatgggt acgcaaggag
    18661 ggctggacaa cgtcaccaaa acctggcgtc cagggtggca aagcccgact ggttcacgtc
    18721 aatgaacagg ttcgtgaata tattcgcaat gctgaacgtc cagaaggtca gggagaagcg
    18781 cctgcccttt ctggtgatgc gccgcttgaa gtattgctgg tgacactggc gaaagagatg
    18841 acgccagttg aacaaaaaca gtttacatcc ttgcttctgc gggaagggat tatcggattg
    18901 ttacaacgct tagggattcg cgatagcaaa taatatgaaa agattacgca ataaaatgac
    18961 caccgaagaa ctggctgaat gcctcggtgt ggctaaacaa actgttaacc gttggatcag
    19021 agaaaaaggc tggaaaacgg aaaaatttcc cggcgtgaaa ggcggtcgtg ccagacttat
    19081 tctggtcgat acgcaagttt gcgagtttat tcagaacacg ccagccttcc ataacacacc
    19141 aatgttgatg gaagctgaag agagaattgc tgaatacgct cctggagcac gcgccccggc
    19201 gtatcgacaa atcatcaacg cgatagataa tatgacagac attgagcagg gaaaagtcgc
    19261 gcaatttttg tcacgtgaag gaattcgcaa tttcctcgcc cgtctcgata ttgacgaatc
    19321 agcataaata aaaaacggca ggatattatc tcctgccgtt tatcttttta cacgctaatt
    19381 actgctgatt ttcgcgttca gcaataaaat ccagcgcttt gttgatacgc tcgatactgc
    19441 gggtcttacc aattgcgtga acggtaacat ccagtgctgg agactgcccc gccccggtta
    19501 cggcgacacg cagcggcata ccaactttac ccatacccac ttccagctca tccgccgtcg
    19561 cctgaatagc gtgatgaacg ttttcagcgg tccagtcagt aatcgcggcc agtttgtcac
    19621 gaaccacttc cagcggctga cgcgctaccg gacgcagatg ttttttcgcc gcatcggcat
    19681 cgaactcagc aaaatcttcg tagaaataac ggcagctctg tgccatctct ttcagcgtct
    19741 tgcagcgttc gcccagcagt ttcaccagat cagccagctg cgggccgtta cgggtatcga
    19801 tattttcctg ctcaatgtgc cactgtaagt gagtagcaac atactccggc ggcagcgcgt
    19861 taatgtagtg atggttcagc cacagcagct tgtcggtgtt gaacgcactg gcagatttgc
    19921 tgacggcatt caaagtgaag tatttgatca tctcttcacg agtgaagatt tcctgatcgc
    19981 cgtgggacca gcccagacgc accagatagt tcagcagtgc ttctggcaaa taaccgtcat
    20041 cacgatactg cattacgctg actgccccgt gacgtttgga cagtttttta ccgtcatcgc
    20101 cattgatcat agaaacgtgc gcgtaaaccg gcaccggcgc tttcagggcc ttaagaatgt
    20161 tgatctggcg cggcgtgttg ttgatatggt cttcgccacg gataacgtgg gtgatttcca
    20221 tatcccagtc atcgacaacc acacagaagt tataggttgg ggaaccatcg gtacggcgga
    20281 taataagatc gtccagttcc tggttgctga actcgatcgg accacggatc tgatcgtcaa
    20341 aaacaacaga accttcctgc gggttagcaa aacgtacaac acacggttca tcatcagcat
    20401 gatgctcatg gctgtggcgg cagcgaccgt cataacgcgg cttctcacct ttcgccattt
    20461 gctcttcgcg cagcgcttcc aggcgctctt tagagcaata gcatttataa gcagtgccct
    20521 cttccagcat ctgatcgatc accgcgttgt agcgatcaaa acgtttggtc tggtagtacg
    20581 gaccttcatc ccactccagg ctcagccagt tcatgccatc cataatggct tcgatagctt
    20641 ccggcgtgga acgctcaaga tcggtgtctt caatacgcag cacgaactca ccgccgtggt
    20701 tacgtgcaaa aagccaggag taaagagcag tacgcgcgcc gccaacgtgc agatagcctg
    20761 ttgggcttgg cgcgaagcga gttttgattt tcatgaaatg gccttacgtt tagaaagatg
    20821 ccgacaaccg gcaaatcctg gaaaaattga gtgggcgata ttctatcact cccgcctgat
    20881 tcctcaatgt agttcgggtt tctaccttac gcattattgt tttcgttgac aaattgcgca
    20941 acacggtctg ttttgcgatc atcttgctta aatttacgac gaacgaacaa tttctttaga
    21001 aaatgcgttg actcattttg aactctccct ataatgcggc tccacacagc gggggtgatt
    21061 agctcagctg ggagagcacc tcccttacaa ggagggggtc ggcggttcga tcccgtcatc
    21121 acccaccaac tactttatgt agtctccgcc gtgtagcaag aaattgagaa gtgggtgatt
    21181 agctcagctg ggagagcacc tcccttacaa ggagggggtc ggcggttcga tcccgtcatc
    21241 acccaccaat ttctcgccag ctaaatttct tgtaaaaatg tgaagtaccg aagtgggtga
    21301 ttagctcagc tgggagagca cctcccttac aaggaggggg tcggcggttc gatcccgtca
    21361 tcacccacca cttcgggtcg ttagctcagt tggtagagca gttgactttt aatcaattgg
    21421 tcgcaggttc gaatcctgca cgacccacca atgtaaaaaa gcgccctaaa ggcgcttttt
    21481 tgctatctgc gatactcaaa gattcga
//
LOCUS       DAZPDU010000062        21160 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZPDU010000062 DAZPDU010000000
VERSION     DAZPDU010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21160
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.063"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            133..735
                     /gene="ais"
                     /locus_tag="ACHURS_002110"
     CDS             133..735
                     /gene="ais"
                     /locus_tag="ACHURS_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFR1237839.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(774..1199)
                     /gene="nudI"
                     /locus_tag="ACHURS_002111"
     CDS             complement(774..1199)
                     /gene="nudI"
                     /locus_tag="ACHURS_002111"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFR1237840.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            1478..2020
                     /locus_tag="ACHURS_002112"
     CDS             1478..2020
                     /locus_tag="ACHURS_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFR1237841.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMHPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            2120..3322
                     /locus_tag="ACHURS_002113"
     CDS             2120..3322
                     /locus_tag="ACHURS_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFR1237842.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVRLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQSPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            3542..4324
                     /locus_tag="ACHURS_002114"
     CDS             3542..4324
                     /locus_tag="ACHURS_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFR1237843.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYPDVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            4339..5544
                     /gene="rhmD"
                     /locus_tag="ACHURS_002115"
     CDS             4339..5544
                     /gene="rhmD"
                     /locus_tag="ACHURS_002115"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050032 - L-rhamnonate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HFR1237844.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQSRQSFGINVLGTLIVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAVMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            5601..6890
                     /locus_tag="ACHURS_002116"
     CDS             5601..6890
                     /locus_tag="ACHURS_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1237845.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVMAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            6908..7711
                     /gene="yfaU"
                     /locus_tag="ACHURS_002117"
     CDS             6908..7711
                     /gene="yfaU"
                     /locus_tag="ACHURS_002117"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HFR1237846.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTSYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVASATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(7752..8654)
                     /locus_tag="ACHURS_002118"
     CDS             complement(7752..8654)
                     /locus_tag="ACHURS_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFR1237847.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVKGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEISERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(8847..10037)
                     /gene="glpC"
                     /locus_tag="ACHURS_002119"
     CDS             complement(8847..10037)
                     /gene="glpC"
                     /locus_tag="ACHURS_002119"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFR1237848.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGIGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(10034..11293)
                     /gene="glpB"
                     /locus_tag="ACHURS_002120"
     CDS             complement(10034..11293)
                     /gene="glpB"
                     /locus_tag="ACHURS_002120"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFR1237849.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(11283..12911)
                     /gene="glpA"
                     /locus_tag="ACHURS_002121"
     CDS             complement(11283..12911)
                     /gene="glpA"
                     /locus_tag="ACHURS_002121"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFR1237850.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            13184..14542
                     /gene="glpT"
                     /locus_tag="ACHURS_002122"
     CDS             13184..14542
                     /gene="glpT"
                     /locus_tag="ACHURS_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFR1237851.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            14547..15623
                     /gene="glpQ"
                     /locus_tag="ACHURS_002123"
     CDS             14547..15623
                     /gene="glpQ"
                     /locus_tag="ACHURS_002123"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFR1237852.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRGHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYSYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(15665..15907)
                     /locus_tag="ACHURS_002124"
                     /pseudo
     CDS             complement(15665..15907)
                     /locus_tag="ACHURS_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            15902..16006
                     /locus_tag="ACHURS_002125"
                     /pseudo
     CDS             15902..16006
                     /locus_tag="ACHURS_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            16086..16735
                     /gene="inaA"
                     /locus_tag="ACHURS_002126"
                     /pseudo
     CDS             16086..16735
                     /gene="inaA"
                     /locus_tag="ACHURS_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
     gene            complement(16789..17043)
                     /gene="yfaE"
                     /locus_tag="ACHURS_002127"
     CDS             complement(16789..17043)
                     /gene="yfaE"
                     /locus_tag="ACHURS_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFR1237853.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(17043..18173)
                     /gene="nrdB"
                     /locus_tag="ACHURS_002128"
     CDS             complement(17043..18173)
                     /gene="nrdB"
                     /locus_tag="ACHURS_002128"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR1237854.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            complement(18407..20692)
                     /gene="nrdA"
                     /locus_tag="ACHURS_002129"
     CDS             complement(18407..20692)
                     /gene="nrdA"
                     /locus_tag="ACHURS_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR1237855.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINNLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            20820..20963
                     /gene="ypaB"
                     /locus_tag="ACHURS_002130"
     CDS             20820..20963
                     /gene="ypaB"
                     /locus_tag="ACHURS_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFR1237856.1"
                     /translation="MTLLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
BASE COUNT         4955 a         5155 c         5692 g         5358 t
ORIGIN      
        1 ttggcaaaat tttgaggcat tttgccgaca tcgtaggatt tttaatatta caccaactgc
       61 taattatcgc cagaaatgtc gctcaatttc acggtaattg tctggttgcg cttgtctata
      121 ggtggagttt acgtgttagc tttttgccgc tcatcattga agtcaaaaaa atatttcatc
      181 attttactgg cgctcgctgc aattgccgga ctgggtactc atgccgcctg gagtagcaat
      241 ggtttgccac gtatcgacaa taaaacattg gccagactgg cacagcagca cccggttgtc
      301 gttttgtttc gccatgctga acgttgcgat cgttcaacca atcaatgctt gtcagataaa
      361 acaggtatta cggttaaagg tacccaggat gcccgtgaac tgggcaacgc ttttagcgct
      421 gacatccctg atttcgatct ttattccagt aataccgtcc ggaccattca gtcggcgacc
      481 tggttttccg cgggtaaaaa actgacggta gataaacgac ttcttcagtg cggtaatgag
      541 atttatagtg caattaagga cttacaaagc aaagcgcctg ataaaaatat cgttattttc
      601 acccataatc attgcctgac atatattgct aaaaataagc gtgatgcgac atttaaacct
      661 gattatctgg atggtttagt catgcatgtg gaaaaaggca aagtttatct ggatggggaa
      721 ttcgttaacc actaaatttt caatctgaca gccagtgatg gctgtcatca ttgttaaaga
      781 agacctttca aacgtaacgt ttttcgggtg gcaacattta aatcataatg caccagatct
      841 tcaggtttta cccacgcgta gtcctgaaac tcttcgttta ttttcacttc tcggttggca
      901 gaaacgcagt caaaaatcag gtaaatcata taaatctctt ccttgcgacc atctgcatac
      961 gtcttggtgc gaatatcatc gctgaaggtc cacggcgtga tttctgtcaa aagcagctgt
     1021 tctcccagtt cttcgcgaat ttcgcggcgt agtgcctctt caattcgttc gccagactcc
     1081 acgccgccac ccgaaagcgc ccattgaccg gggaaaacgc cgcgatcgtc ggccattttg
     1141 cacagcaaat aagcaccatc attttgaatc aaagggcata caatagtccg ttgtcgcaca
     1201 ggggtattcc ttataaataa aaagaaacca tgagaatacg gtaactcaga aatatgtgaa
     1261 agtgacagta atctcttttg cgatgataac tgcacttatt attatttttg tgtgtatcgg
     1321 taaagtgtgt ttattttttc ggcgtttaat tatttaagaa gtatttagtt acaataagtg
     1381 actagatccc ttttttcgct cattgaatcc tctgtgccag gcagtacaat cccgcagctt
     1441 tgggaatttt gattaattgc ttgtgggaaa ggggataatg aaaaaaattg cgctagcagg
     1501 tctggccgga atgctgttgg tttctgcatc ggtcaatgca atgagcatca gcggtcaggc
     1561 gggtaaagaa tacaccaata ttggtgtcgg ttttgggact gaatcgacgg gcctggcgtt
     1621 aagcggtaac tggacacata acgacgacga cggtgacgtc gctggcgtgg ggctggggtt
     1681 gaatctgcct ctcgggccgt taatggcgac cgttggcgga aaaggcgttt acaccaaccc
     1741 gaattacggc gatgaaggtt atgccgcagc ggtaggaggt ggtttgcagt ggaaaattgg
     1801 caacagcttc cgtttgtttg gcgagtatta ctactctccg gattcactct ccagcggtat
     1861 taaaagttat gaggaagcga atgctggcgc gcgttacacc attatgcatc cagtcagtat
     1921 tgaggcgggt tatcgctacc tgaatctgtc gggtaaagac ggtaaccgcg acaacgccgt
     1981 ggctgacggc ccgtatgttg gggttaacgc cagtttctga tcccttccgg cgcggcattc
     2041 tgtcgcgcca ttcagtttct tactacgcct tatctccctt tgcgttatag tgattcgata
     2101 acaaaagcag gagataacca tgttaaaagt ggaaatgtta tccaccgggg atgaagtgtt
     2161 acacgggcaa atcgttgaca ctaacgctgc ctggctggcc gattttttct ttcatcaggg
     2221 gttgccatta tctcgccgca atacggtggg ggataacctt gatgacttag tcaccattct
     2281 tcgcgaacgt agtcagcacg ccgatgtgct gatcgttaac ggcgggctgg gaccgaccag
     2341 tgatgattta agtgcactcg ccgctgcgac agcaaaaggt gaaggcctgg tgctgcatga
     2401 agcctggctc aaagagatgg aacgctattt ccacgaacgt ggacgagtaa tggcaccgag
     2461 caaccgtaaa caagcggagc tgcctgccag tgctgaattt atcaataacc cggtaggcac
     2521 cgcctgtggt tttgccgtgc ggcttaatcg ttgcctgatg ttctttactc ccggcgtacc
     2581 gtcagaattt aaggtgatgg tcgagcacga aatcctgccg cgcctgcgcg agcgtttttc
     2641 tttaccacag tcgccggttt gtctgcgttt aactaccttt ggtcgttcgg aaagcgatct
     2701 ggcacaaagc ctggacactc tacaactgcc gccgggtgta acaatgggct atcgctcctc
     2761 aatgccgatc atcgaactga aactcaccgg accggcaagc gagcaacagg cgatggaaaa
     2821 actgtggctg gacgttaaac gtgttgccgg acagagcgtg attttcgaag gcaccgaagg
     2881 actgcccgcg cagatcagtc gcgaattgca aaaccgccag ttcagcctga cgttgagcga
     2941 gcaattcacc ggtggtttat tggctttgca actttctcgc gcaggtgctc cattgctggc
     3001 gtgtgaagtg gttccttcac aggaagaaac cctggcgcaa actgcgcact ggattacaga
     3061 acggcgggcc aaccattttg ccgggctggc actggctgtt tcgggtttcg agaacgagca
     3121 tctcaacttt gcgctagcca cgccagacgg cactttcgct ctgcgtgtgc gtttcagcac
     3181 tacacgctac agcctggcta tccgtcagga agtgtgcgca atgatggcac tgaatatgct
     3241 gcgccgttgg ttaaacggcc aggatatcgc cagtgagcat ggctggattg aggttgttga
     3301 gtccatgacc ttatctgtct gattccgcca ctctgtgccg gtttactgca ccggcacatt
     3361 ccccctccgg caaaacttgt ccaacctgtt gtactcaata agaaaaaatc cgtgcactga
     3421 ttcacaaatt tgtgcgtaat ttctccagtc cgctggacga ggagcgggat ttgatgcgct
     3481 tcacaatttt actccccctg agcaacgaga ctgattccag tatctaagac tggagtccag
     3541 tatgttggaa tcaagcaaag ttcccgccct gacacgggct attgatattc tcaatttgat
     3601 tgcccgcatt ggcccctgta gtgctgcgac gatcattgac acattgggaa tccctaaaag
     3661 cacggcctat ttgctgctta atgaactcag gcgtcagcgt tttcttagcc ttgatcacca
     3721 ggaaaatttt tgtttgtgga ccaggctggt ggagctttct ggtcatgcgt taagcaagat
     3781 ggatctccgg gaactggctc gaccgcgttt gacgcaactg atggatacca ccgggctgtt
     3841 atgtcacctg gggattattg ataacggcag cgcttattac attttaaaag tggagtcatc
     3901 agccactatc agcgtgcgtt cccatgaagg aaaaagcctt tcgctttatc gctccggaat
     3961 aggtaaatgc ctgcttgcct ggcaacctgc agcggtacag caaagtatta tcgaagggct
     4021 ggtctgggag caggcgacac ccacaacgat tactcatcca cagcaacttc acgaagaact
     4081 ggcgcgtatt cgccgtcagg gctggagcta tgacaacggc gaagattacc ccgatgtccg
     4141 ttgtgtcgct gcgccggtgt ttaacgccaa taacgagctt accgctgcga tctcggtggt
     4201 cggtacccgc ttacaaatca atgaagaata ccgtgattat ctggcgggca aggccattgc
     4261 ctgcgccagg gatatttcac gtctgttggg atggaaaagt ccctttgact tacaggcctc
     4321 ataacaatgg agaacatcat gaccctacca aaaatcaaac aggttcgcgc ctggtttact
     4381 ggcggtgcga cagcagaaaa aggtgctggc ggcggtgatt atcacgacca gggggcgaat
     4441 cactggattg acgatcatat tgccaccccg atgagtaaat accgcgatta cgagcagtca
     4501 cgccagtcat ttggcattaa cgttcttggc acgttgattg ttgaagtcga agcagaaaac
     4561 ggccagaccg gattcgctgt ttcgacagcc ggtgaaatgg gctgttttat tgtcgaaaaa
     4621 catcttaacc gtttcattga gggtaaatgt gtcagtgata tcaaactgat ccatgatcaa
     4681 atgctcaatg ccaccctgta ttactccggc tctggtggcc tggtgatgaa cacgatttcc
     4741 tgtgtcgatc tggctctgtg ggatctgttc ggcaaagtgg tcgggcttcc ggtttataaa
     4801 cttttaggcg gcgcagttcg tgatgagatt cagttctacg ccacaggcgc gcgtccggat
     4861 ctggcaaaag agatgggctt tatcggtggc aaaatgccga cgcactgggg gccacatgat
     4921 ggcgatgcgg ggatccgcaa agatgccgtt atggtcgcgg atatgcgtga aaaatgcggt
     4981 gaggatttct ggttaatgct cgactgctgg atgagtcagg acgtgaacta tgccaccaaa
     5041 ctggcccacg cttgcgcgcc ctataacctg aaatggatcg aagagtgcct gccgccacag
     5101 cagtatgaag gttatcgcga actgaaacgc aacgcgccag tcggaatgat ggtcaccagc
     5161 ggtgagcacc acggcacact gcaatctttt cgtacgcttt cagaaaccgg tatcgacatt
     5221 atgcagccgg atgttggctg gtgcggtggt ttaaccacgc tggtggaaat tgccgcaatc
     5281 gccaaatcca gggggcaact ggtggtgccg cacggttcgt ctgtttactc tcaccatgcg
     5341 gtgatcacct tcaccaatac gccattcagc gaattcctga tgaccagccc ggattgttca
     5401 acgatgcgtc cgcagtttga cccgattctg ctcaatgagc cggttccggt gaatggtcgt
     5461 attcataaat cagtgcttga taaacccggt tttggcgtcg aactcaatcg tgactgcaat
     5521 ttgaaacgcc cctacagcca ctaatcactc cgggcgttgc tgtgacaacg gtaacgccca
     5581 catattctaa ggagcttttt atgagcaccg ctttgcttga cgccgtggtg aagaaaaacc
     5641 gtgtgcgttt aattccgttt atgttggcgc tgtatgtgct ggcgtttctc gaccgttcga
     5701 atatcggttt tgccaaacag acctaccaga ttgataccgg gttgagtaat gaagcttatg
     5761 cgctgggagc aggcattttc tttgtggtat atgcgtttct gggtgttccg gcgaatcttt
     5821 tgatgcgcaa actgggggcc agaacctgga ttggtacgac aacactgctg tggggatttc
     5881 tttcggcagc catggcatgg gccgatactg aagcgaaatt tctgatagtt cgcactctgc
     5941 ttggtgctgc ggaggccgga tttttccctg gtatgattta tctcacctcg caatggtttc
     6001 cgcagcgtaa tcgcgccagc attatggggc tgttctatat gggcgcaccg ctggcgttaa
     6061 cactgggatc accgctttcc ggcgcgctgt tggagatgca tggatttatg gggcatcccg
     6121 gctggttctg gatgtttgtg attgaaggat tgttggcagt cggtgctggg gtattcacat
     6181 tcttttggct tgatgacaca ccggagcagg cacgttttct gagtaaacaa gaaaaaacgt
     6241 tgcttatcaa tcaactggca agtgaagaac aacagaaagt gacttctcgg ctgagcgatg
     6301 cgctgcgtaa tggccgagtc tggcaactag cgattatcta cctgaccatt caggtggcgg
     6361 tttacggatt aattttcttc ctgccgaccc aggttgcggc attgctggga acaaaagtgg
     6421 gctttacagc gtcggtggtc accgccattc cgtgggttgc ggccttgttt gggacctggc
     6481 ttattccgcg ctattccgat aaaaccggcg aacggcgtaa tgtcgcagcg ctgacattac
     6541 tggcggcggg cattggtatt ggtctgtccg ggctgctttc tccagtaatg gcgatcgtag
     6601 cgctgtgtgt tgcagctatc gggtttattg ccgtgcagcc ggtgttctgg acgatgccga
     6661 cacaactgct ttccggtacg gcgctggctg cggggattgg ttttgtaaac ctgtttggtg
     6721 cagtgggcgg gtttattgcc ccgatcctgc gcgtgaaagc agaaacgtta tttgccagcg
     6781 atgcggcagg attactgaca ctggcagcgg tggcggtcat cggttcgctg attattttca
     6841 ctctgcgtgt aaatcgtact gttgcgcaga ccgacgtggc acatcattaa ataggttaag
     6901 gaacacgatg aacgcattat taaccaatcc ctttaaagaa cgtttacgca agggcgaagt
     6961 gcaaattggt ctgtggttaa gctcgactac ttcttatatg gcagaaattg ccgctacttc
     7021 cggttatgac tggttgctga ttgacgggga gcacgcgcca aacaccattc aggatcttta
     7081 tcaccagcta caggcggtag cgccctatgc cagccaaccc gtgatccgtc cggtggaagg
     7141 cagtaaatcg ctgattaaac aagttctgga tattggcgcg caaactctat tgatcccgat
     7201 ggtcgatact gccgatcagg ctcgtcaggt ggcgtctgcc acgcgctatc ctccctacgg
     7261 tgagcgtggt gttggggcca gtgtggcacg ggctgcgcgc tggggacgca ttgagaatta
     7321 catggcgcaa gtcaacgatt cgctttgtct gttagtgcag gtggaaagta aaacggcact
     7381 ggataacctg gacgagatcc tcgacgtcga agggattgat ggcgtgttta ttggaccagc
     7441 ggatctttct gcgtcgttgg gctacccgga taacgccggg cacccggaag tgcagcgaat
     7501 tattgaaacc agcattcggc ggatccgtgc tgcgggtaaa gcggctggtt ttctggctgt
     7561 ggctcctgat atggcgcagc aatgcctggc gtggggagcg aactttgtcg ctgttggcgt
     7621 tgacacgatg ctctacagcg atgccctgga tcaacgactg gcgatgttta aatcaggcaa
     7681 aaatgggcca cgcataaaag gtagttattg atatcaaagg cccatgggga tcggctgtgg
     7741 gcctgtgtta attagtggtt attcgctgcc agatcggctt cgcttagctg ggtggccgcg
     7801 agcacctggt cacgatcaat gccctgttcc agcatgcgca aagcaatttt aatggcttgt
     7861 tcatgcatac cttcctgcca gccaatttgg tgtccttcct gccgtagccg ctcagcaata
     7921 gtcatcaatc tctccttttg tagtggtgaa cgttcggaaa tctcctcaat aaaacgggtg
     7981 aaacgggagg tatcgccgca ttgcagcaga taattaaaca gtgtttgtag ctggctgtca
     8041 ttagtgaagc ctttaaccaa aagcgtggtg atcctgtcga ccataccgat taaatcgcgg
     8101 tcgcgaatat gcttttgaat cagttcaagc agagctatac gccgatgttg catgatctca
     8161 tcgtcaggta cgatggtgat atccaccaac ggaaaagctt cggtgtacaa ctgccgggca
     8221 agttgcggat cgtcaaactc atccagccag ttaagtgagt acgggtaggg cgatgtttcg
     8281 ccatgataaa acagcaatgg caccaccagc ggaactctgt catagccttt atccaggtga
     8341 cgctgcatgg cggcagtggc atagcgcatt agccgaaaag ccatattctt ttctgcagag
     8401 ctttgatgtt caatcacgca gtagatatag ccgtcaccgt cgctggtttc cacggaccac
     8461 aaaacatccg agtagtaagc gcgtaaactt ttttcaataa aactagtggg ttccaggcgt
     8521 aaggtttgca ggttgcaaag cttgcgcagt ggttctggca aatgtatttc gagaaaatcc
     8581 cgtgcggttt cgggtgtgaa cataaaggtt ttaaataccg catcatgcgg ggaggaggtt
     8641 gttgattcgg tcatggttgt ccgtcacctg atatttatga tgacgcgaca ttacggttat
     8701 cccaggcctt tgacatctgc cagtttcagc ctttgcgaga cgttttcaga ggaattttca
     8761 ctgcgttgca acgcatacat tgggcacgga atcgtgtacg caaaaatgcc cggtaaccac
     8821 atgcttaccg ggcatcagaa aggagtttaa gccagcgcct gggccagtag cgtaatcgga
     8881 tgttcgcagc gaagacttgt ggacatctca atctgccatt tacaggtttc gcagtcggtg
     8941 accaccagat ctgcgccgct ttcttctatc tggcggaaca gtggtgcgcc gatggcttgt
     9001 gaggtggggt agttctcttt tttgaaaccg taagtacccg caataccgca gcactgggaa
     9061 tccagcaccg ttaactcaag ccccgggatt ttacgcaaca gctccagggt gtagagcgtc
     9121 cagcccattt tttccatatg gcacggagtg tgataaacca ctttcagcgg cagcggtttc
     9181 agcggtaacg ttttgccttc gtccagcttg cgccacagcc agcgggttgc cagttcgata
     9241 tgatcgcgca agcctttgtt gtcgacattc agcacttccg ggtattcgtc gcgcagggca
     9301 aatgtacagg ttgaggaggt ggcaatcacc ggaatgcctt ttactcccac agcttcgcgg
     9361 atcgactcta cattcgtaat tgcctgtttg cgtgctttat cggtaaagcc gttggcgatt
     9421 agcggtacgc cgcagcattt ttctttgctg agcagttgta caccgatacc cattgcgttg
     9481 agcactttaa ttaaatcttt acctaactgc ggatggttgt agttaacgaa gcagccgtga
     9541 aagaaagcga cctggtcttt atattgtgct tgctgagccg ctatgctgcg ataccagcga
     9601 cggaacgtgc cgaaggagta tttcggtagc gtgcgacgat gatcgatttt taacgccgca
     9661 tcaagcagct gccgcaccgg tttcagcgag gtagcggtgt tgacgattgg tgcgaacggc
     9721 gtggaaacgc tacccatcag gtcggtatga ctcaacacaa aattacgcag cgacgggcgc
     9781 gtggtgtcat attttgcccg cgcgcgctgg ataatatcgc caatcttcac atcggacggg
     9841 caggcgactt cacaacgttt gcagttgatg caatatttca gcgcctcgtc atacagtgcg
     9901 ccatctttca aacgcagacg ctcgccatcc ggcccggctt gttttggccc tggataaccg
     9961 ggattcaccc ggctcaccgg gcaggcggtg gtgcagacgg tgcacttaat gcagttttcg
    10021 aagctggtgt cgttcattgt tggcctcctg cgcgttgggc aatctgttga gcggcatgta
    10081 aagcactgac ggcacaaaca ccgccgccgc atccctgggc gatgggatca aatccgccca
    10141 gcaccgaacc gatggcaaac aggttctcaa tggtttgccc tgcctgcgac gggcgtagcg
    10201 tctcatcagt ggttacaccg aactgctgcc acggttgcgg cgcaaaaaaa tcgcccttat
    10261 accattcacc ccgcgtggcg gtttgtagca catcaaggcc gagaatcggc tcgcgaatgc
    10321 cgttacgttc cgctaccagt ccgccactaa agaaactgcc gctggcgaga accgcgaaac
    10381 gtggacgtag cggaatatcg gcgtgattgc gggtccagat ttcattcact acgccatttt
    10441 tacaggtcac ttttttcact tcatcgcccg gcatccacac gccaccctgg cgcacaaact
    10501 ggcgctgtaa ctggttttgc agacgaatgc ccagcacgga aggcggcagc gttggcaaaa
    10561 gcatcagtga acaaggtagt ttttcattca accaacgcca cagtttgtca tcggccaggc
    10621 cgaagcaggc gggcatcagg atcatttcgc aggtattggc gacaggaata agcgcatcaa
    10681 gtaacagcgg ccagttttct tcattatcaa ggaaacgggc gatattcacc gcgcgaaatt
    10741 cggtggcgtt attgcgcagg acatccagtt ccggcagctc tatttctgcg gtttcaaccg
    10801 aaagatccag ttcgcgcaac gaagccgccg caagatgcgc ctggaaatcc atcagcccgc
    10861 ttatccccac tacacatatc ttcttcgcgg gtagcggcca gacgggaact tctggcgaac
    10921 ttaaccaggt tgcacgcaaa gtgccgagcg gggtaacgcg ctgatgggcc agttccacgc
    10981 tgccctgcaa ttgcgcaccg ctttcagcga tcaatgcctg cgcctggcaa gcgagatcga
    11041 gcacgcgttg tggttcgaga agactgtaag gatgggctgg tgcctgctga cgcaaagatt
    11101 ccagtccact gtgaatgtct gtcaccggtt gaccatcagg cagatggctc agcaaatcca
    11161 gcgaaccgga tgagaaatgc agcgcgcttt gaccacgcgt gacaatggca cagcgcaggc
    11221 cgtgtttttg cagttgcagg ccacagagta atccggcgag gccgccgccc ataatgacag
    11281 tatcaaagcg catctttctg ctccttctcc agaccacaca gtccctgata aacccagcgg
    11341 gtaaattcgc tttcgcgcag tgcatctccc caggcgatgg gttgcacgcc tttccagcgt
    11401 tcgttaagga aggtggaaag ttgctcgata gattgcgcgg acgtcgtgac gttaaaacgt
    11461 tgcagcagtc cggcagcgcg gcaggcgcag agttcgccct ggcaggtacc catccccaca
    11521 cgggtacgac ggcgtaaatc cagcaggcta ttaacgttta aattttctac cgcgtactgc
    11581 acttcacccg ctgttaccgc ttcgcactca cataccaggc tacggtgcag acgaccttcg
    11641 ctcagccagg caggcgtgcg atcgccatga cgataaaccg cagaaccgcg cagcggggca
    11701 ggcagggaga tgactttacg caaggtaact tcagcgggtt cctgtgaacc aggaagtgcc
    11761 agatcggcgg tcgtacaggg gcgcgtgttg cccagtttgc ggcacacggc gtcggtagcc
    11821 cattcagcca tcagccgata ggtcatcagt ttgccaccgg tgatggtgat aaatccgtcc
    11881 agaccatcgc gttcagcatg gtcgagcagc acgatgccac ggctgacgtt acgtccgctc
    11941 gggtcgtcat cgctggcaac cagcgggcgc acgccagaat aggcccgcaa aatgcgcgtt
    12001 ttcgccatca cgggggccag tttttcccct tcacgcagca gaatatcaac ctcttctgcc
    12061 gtcactcgat tatcgtcaat ctcgttgtaa tcaatacgta aagaggtggt gccaatcagc
    12121 gaaatggtat cgccaggcac cagaatatcg gcgtcggaag gtttacggca gcggttgatc
    12181 acatgctggt taatgcggtg atccatgatc agcagcgatc ctttcgccgg gaacatgcga
    12241 atgcgcagat cggcatattc ggcaatgtgt tgcccccaga tcccggcggc attaaccacg
    12301 acaggcgcat gaagggcctg agtttcgccg gtgagatggt tacgtacacg aacaccgcac
    12361 accgtcgcgc cttcacgaat cagtcccgtg acttcatgag cggtaaggat aacggcaccg
    12421 tgttctttgg catccagcat gtttgctgcg gtcagacgaa atggatcaac ggtgccatcc
    12481 ggaactttca ccgcgccaat caatgccggg ttaacggcag gttcgataat gcgcgcttgc
    12541 tgcgggtcta tagcttctgc gctgatccct gcttcttcgc aggcgcgaat aaaagtggcc
    12601 tggaaggaga ggtcatcttc cggcagggta ataaacaggc cgttggttgg ttcaacgcag
    12661 tgacgtgcaa tgcgtttcag gatctggttt tcactaatgc attcgcgggc cgattccgca
    12721 tcggttaccg catagcgcgc accgctgtgt agcaggccgt ggttacgccc ggtggcaccg
    12781 gttgcgatgt cgtggcgctc aaccaaaatc acgcgcagcc cgcgcagggc acagtcgcgg
    12841 gcaatcccgg ctcccgttgc gccgccgcca atgataatca cgtcacttga ttgcgagtcg
    12901 cgagttttca ttgttatccc tctgaagttc gttttttacc atttagccat agtaaaaaca
    12961 tgaattgttt gatttcgcgc atattcgctc atgattcgaa agtgaaacgt gatttcatgc
    13021 gtcattttga acattttgta aatcttattt aataatgtgt gcggcaattc acatttaatt
    13081 tatgaatgtt ttcttaacat cgcggcaact caagaaacgg caggttctct cactgaatca
    13141 ggctgttaat cataaataag accacgggcc acggaggcta tcaatgttga gtatttttaa
    13201 accagcgcca cacaaagcgc gcttacctgc cgcggagatc gatccgactt atcgtcgatt
    13261 gcgctggcaa attttcctgg ggatattctt tggctatgcg gcttactatt tggttcgtaa
    13321 gaactttgcg cttgctatgc cttatctggt tgagcaggga ttctcacgcg gtgatttagg
    13381 ttttgccctt tcggggatct cgattgctta tggattttcg aaattcatca tgggttcggt
    13441 atcggatcgc tcgaatccgc gcgttttcct gcccgcaggt ttgattctgg cggcggcagt
    13501 gatgttgttt atgggctttg tgccatgggc gacgtcgagc attgcggtga tgtttgtact
    13561 gttgttcctc tgcggttggt tccaggggat ggggtggccg ccgtgtggtc gtactatggt
    13621 gcactggtgg tcgcagaaag aacgtggcgg cattgtgtca gtgtggaact gtgcgcacaa
    13681 cgtcggtggt ggtattccgc cgctgctgtt cctgctgggg atggcctggt tcaatgactg
    13741 gcatgcggcg ctctatatgc ctgctttctg cgccattctg gtggcattat tcgcctttgc
    13801 gatgatgcgc gataccccgc aatcctgtgg cttgccgccg atcgaagagt acaaaaatga
    13861 ttatccggac gactataacg aaaaagcgga acaggagctg acggcgaagc aaatcttcat
    13921 gcagtacgta ctgccgaaca aactgctgtg gtatatcgcc atcgccaacg tgttcgttta
    13981 tctgctgcgt tacggcatcc tcgactggtc accgacttat ctgaaagagg ttaagcattt
    14041 cgcgctggat aaatcctcct gggcctactt cctttatgaa tatgcaggta ttccgggcac
    14101 tctgctgtgc ggctggatgt cggataaagt cttccgtggc aaccgtgggg caaccggcgt
    14161 tttctttatg acactggtga ccatcgcgac tatcgtttac tggatgaacc cggcaggtaa
    14221 cccaaccgtc gatatgattt gtatgattgt tatcggcttc ctgatctacg gtcctgtgat
    14281 gctgatcggt ctgcatgcgc tggaactggc accgaaaaaa gcggcaggta cggcagcggg
    14341 ctttaccggg ctgtttggtt acctgggcgg ttcggtggcg gcgagcgcga ttgttggcta
    14401 caccgtggac ttcttcggct gggatggcgg ctttatggta atgattggcg gcagcattct
    14461 ggcggttatc ttgttgattg ttgtgatgat tggcgaaaaa cgtcgccatg aacaattact
    14521 gcaaaaacgc aacggaggct aatggcatga aattgaagct gaaaaacctt agcatggcga
    14581 tcatgatgag cactatagtc atgggaagca gtgcaatggc ggcggacagc aacgaaaaaa
    14641 tagtcatcgc ccatcgcggt gccagtggat atttgccgga gcatacgctg ccagcaaaag
    14701 cgatggcgta tgcgcaggga gcggattatc tggaacagga tttggtgatg accaaagacg
    14761 accatctggt tgttctgcat gaccattatc tcgatcgtgt tactgatgtt gccgatcgtt
    14821 tcccggatcg ggcgcgcaaa gacggtcgtt actacgcgat agatttcacg ctggatgaaa
    14881 ttaagtcgct gaaatttacc gaaggtttcg atattgaaaa cggtaaaaaa gtacagactt
    14941 atccggggcg tttcccaatg ggtaagtccg acttccgggg gcacaccttt gaagaagaga
    15001 ttgaatttgt tcaggggtta aatcactcta ccgggaaaaa tatcggtatc tatccagaaa
    15061 tcaaagcgcc gtggttccat catcaggaag ggaaggatat tgcggcaaaa acgctggaag
    15121 tgctgaagaa atatggttac accggtaagg acgataaagt ttatttgcaa tgttttgatg
    15181 ctgatgagct gaagcgtatt aagaatgagc tggaacctaa aatgggcatg gatctcaatc
    15241 tggtacagct gattgcctat accgactgga atgaaacgca gcagaaacag ccggacggaa
    15301 gctgggttaa ttacagctat gactggatgt ttaagccagg tgccatgaag caagtcgcgg
    15361 aatatgccga cggtatcggg ccggattacc atatgttgat tgaggaaaca tcgcagccgg
    15421 gtaatatcaa actcactggc atggtgcaag atgctcagca gaataaactg gtagtgcatc
    15481 cttataccgt gcggtcggat aaactgcctg aatacactac tgatgtgaat cagttatatg
    15541 atgctctgta taacaaagcg ggtgtaaatg ggctgtttac tgatttccct gataaggcag
    15601 taaaattcct taataaagag taatgctact gcaggatggc gttcatgcgc catcctgtct
    15661 catatcaaat ttttccaatc gccactaatt gttccatgca catttgtagt aaaaacagtg
    15721 gactgccttc taccggcttt tctttagcca acaacgaaat ctgtcgatag aaggttggtt
    15781 cgtgaggaac ggaacacaat tcacctgcaa tggttttcag cgttaactct ggtaataagg
    15841 cgatgcctaa tccctgtcga ataaaattca tcgctgtatc cacctgatta aattcacatt
    15901 ttatctgctg ggcgcactat tagtggtcgt ggctggtgga atctatgcgc tgcaaatgaa
    15961 gggcaaaagc ggtgtcacac tagtagcggc aaaagaaatt cattaatacg acacgtttca
    16021 ttaagatttt cctcaggtaa ccacctatag tcattgtcgt taataaaaat gacaggcgat
    16081 agggtatggc agtttcagca aagtacgacg aatttaatca ctggtgggca acagagggcg
    16141 actgggttga agagcctaac tatcgccgta atggtatgag cggggtgcaa tgcgtcgagc
    16201 gcaacggcaa aaagctgtat gtaaagcgca tgacgcatca tctgtttcat tccgtacgtt
    16261 atccgttcgg ccgaccaacg attgtccgtg aggttgcggt aattaaagaa ctggaacggg
    16321 ccggtgtcat cgtgccgaag atcgtttttg gtgaagcggt gaaaattgag ggtgaatggc
    16381 gcgcgttgct ggtgactgaa gatatggcgg ggttcatcag cattgctgac tggtatgccc
    16441 ggcatgcagt atcgccctat tctgacgaag tacggcaagc catgttgaaa gcagtggcgc
    16501 tggcgtttaa gaaaatgcat agcgttaatc gtcagcatgg ctgttgttat gttcgccata
    16561 tctatgtgaa aacagaaggt aaagcagaag ctggttttct tgacctggag aaaagtcgtc
    16621 gccgcttgcg tcgcaataaa gcgattaatc atgacttccg ccagctggaa aatatctgga
    16681 gccaatcccc aaagcggact gggagcaggt gaaagcgtat tactacgcca tgtaatcgtt
    16741 gtaggccaga taagacgtat aagcgttgta tctggcacta caaccaattc acatctcgat
    16801 ttcaatatcg ccttttgccc ggcaacaaca gggcaaaatt tcccccggct gaataaaggc
    16861 taacggttcg gcaatccagt ccacctgacc tgcgaccagt cgggtgcgac aggagccgca
    16921 gtaaccttcg cggcactggt actcaaccgc aacattgtgg gattccagcg ccgccagaag
    16981 ggaagggtgt tcatcctggc acagcagttg tgtgccagtg atgcgcaggg taacgcgggc
    17041 catcagagct ggaagttact caaatcgtcg gtgtccactt ccgcgtcaat ctgcccgacc
    17101 agataagaac tgacttccac ttcctgcgga gcaacctgca cgttatcaga caccagccag
    17161 gtgttgatcc acgggatcgg gttggagcgc gtctggaacg gcagatccaa accgactgcc
    17221 tgcatgcgga tattggtgat gtattcaacg tactggcaga gaatatcttt attcagacca
    17281 atcatcgaac cgtcacggaa cagataatcc gcccagtctt tctcttgctg tgccgcctgg
    17341 acaaacaggt catagcactc ctgcttacat tcttcggcaa tttccgccat ctccggatca
    17401 tccgcgccgc tgcgcagcag attcagcata tgctgagtac cggtcaggtg cagggcttca
    17461 tcgcgggcaa tcaggcgaat aatcttggcg ttaccttcca tcagttcgcg ttctgcaaag
    17521 gcaaaggaac aagcaaagct gacgtagaag cggatcgctt ccagcgcgtt aacgctcatc
    17581 aggcagagat acagtttttt cttcagctcg cgcaggctaa cggttacggt tttaccgtta
    17641 acggtgtggg tgccttcgcc cagaaggtgc cagtagctgg tcatttcgat cagttcatcg
    17701 taatagctgg agatcccttc cgcacgtttc tggatctgct cgttggtgac gatatcgtca
    17761 aacacaacag acggatcgtt aacgatatta cgaatgatat gagtatagga acgggaatga
    17821 atcgtttctg agaacgccca ggtttcgacc caggtttcca gttccggaat agaaataagc
    17881 ggcaatagcg ccacgttcgg gctacgaccc tgaatggaat ccagcagcgt ctgatatttc
    17941 aggttgctga taaagatgtg tttttcgtgc tccggcagcg cctggtaatc tatacggtcg
    18001 cgggagacgt caacttcttc cggacgccag aagaaagaga gctgcttttc gatcagcttt
    18061 tcgaagatgt catatttttg ctgatcgtag cgagccacgt tgaccggctg accaaagaac
    18121 atcggttctt tgagctgatc atttttcgtc tgtgaaaagg tggtatatgc catgagtgtg
    18181 tcctgttggg agtttaatgc cggataaggc attttacgcc gcatcctgtg cctgatgcga
    18241 cgctggcgcg tcttatcagg cctacaaatc cgagccgtag gccggataag gcgttcacgc
    18301 tgcatccggc acccggagcc tgatgcgacg ctggcgcgtc ttatcaggcc tacaaaccga
    18361 gccgtaggcc ggataaggcg tttacgccgc atccggcatc tcaatatcag atcttacatg
    18421 cgccgctttc gcagccatcg tcctggattg acggtaccag atcgtcctgc gcgtcttcag
    18481 cgccgtcacg ggtgttctga taatacagcg ttttgacccc gaatttgtag gcggtgagca
    18541 ggtctttcag caactgctgc atcggcactt ttcctgacgg gaaacgtgac ggatcgtagt
    18601 tggtgttagc agagatcgac tgatcgataa atttctgcat gatacctacc agttgcaggt
    18661 agccatcgtt acccggcatt tcccacagca gctcataggc gtcgtgcagg tgctcgtagt
    18721 ccggcaccac ctggcgcaaa ataccgtctt tcgacgcttt gatgctgacg taaccgcgcg
    18781 gcggttcaat accgttagtg gcgttagaga tctgcgaaga ggtctcggac ggcatcaggg
    18841 cagaaagcgt agagttacgc aggccgtgcg ttttgattga ttcacgcaat gcttcccagt
    18901 cgtaatgcag cggctcatta gcgatggtat ccagatcttt cttataggta tcgatcggca
    18961 ggatcccttt cgcgtaagtg gtttcgttaa accacgggca cgcgccttgc tctttcgcca
    19021 gctcattaga ggctttcagc aggtaatact gaatggcttc gaaggtttta tgcgtcaggt
    19081 tgttggcgct gccgtcggag tagcgtttac cgtgcttcgc caggtagtaa gcgaagttga
    19141 tcacaccaat acccagcgta cgacgaccca tcgctccacg tttggcggcc gggatcgggt
    19201 aatcctgata atccagcagc gcgtcaagtg cacgaaccgc cagaattgcc agctcttcca
    19261 gttcatccag gttattaatt gcgcccaggt tgaaagcaga cagcgtacac agcgcgattt
    19321 caccgttctc gtcgttgacg tcgttcagcg gtttggtcgg cagggctatc tccaggcaca
    19381 ggttagactg acgcactggc gcgatggccg gatcaaacgg gctatgggta ttgcagtggt
    19441 caacgttctg aatatagata cgaccggtag acgcacgttc ctgcatcatc agcgagaaca
    19501 gctcaacggc tttcacacgc tgcttgcgga tgctgtcgtc tttctcatat ttggtataca
    19561 gacgttcaaa ctcttcctga tcggcgaaga acgcgtcgta cagccccggt acgtcggacg
    19621 ggctgaacag ggtgatatct tcacctttca gcagacgggt atacatcagt ttgttgattt
    19681 gtaccccgta gtccatatga cgcacgcggt tgccttccac accacggttg tttttcaaca
    19741 ccagcaggct ttccacttcc agatgccaca tcgggtagaa cagcgttgcc gcaccgccgc
    19801 gcacaccgcc ctgagagcag gatttcaccg ctgtctggaa atgtttgtag aacggaatgc
    19861 agccggtatg gaacgcttca ccaccgcgaa tcgggctacc cagcgcacga atacgcccgg
    19921 cgttgatgcc gatcccggca cgctgggaaa cgtatttaac aatcgcgctg gaggtggcgt
    19981 tgatggaatc caggctgtca ccgcactcga tcagtacgca ggagctgaac tgacgagtcg
    20041 gggtacgcac gccggacatg attggcgtcg gcagcgaaat tttaaatgtg gaaaccgcgt
    20101 cgtaaaaacg cttcacatat tgcaggcgcg tttcacgcgg gtagttcgag aacaggcacg
    20161 cggcaaccag aatataaagg aactgggcgc tctcatagat ttcgccggtc acgcggttct
    20221 gtaccagata tttgccttcc agctgcttaa cggcagcata agagaaggtc atatcacggt
    20281 cgtgatcgat aaacgtgtcc atctgcttga actcttcttc cgtgtagtct tccagcagat
    20341 gattatcgta tttgcccatc tcgaccattt tcaccacgtg gtcgtacagc gcaggcggct
    20401 caaactggcc gtaggctttt ttacgcaggt ggaagatcgc caggcgcgcg gcgagatact
    20461 gataatccgg cgcatcacgg gagatcaggt ctgcggcagc cttgataatg gtttcgtgga
    20521 tgtcagaggt cttgataccg tcataaaact gaatgtggga gcgcagctcg acctgggaaa
    20581 tcgaaacgtt atgcagtcct tctgccgccc aatccagaac gcgatggatt ttgtcgagat
    20641 tgatgcgctc tgtgctaccg tcgcgctttg tcaccagcag attctgattc atgtatgtcg
    20701 tacctgtttt tggaattctt tccgcaatag tttcatgcag actacctgta gtaagggaag
    20761 ctgcttagaa cactatatat agggggtatg tttgggattc actgcaagat agtgtgaaaa
    20821 tgaccctctt gcaagtgcat aactttgtgg ataactcagg aaggaaaaag tggctttcgc
    20881 gcaccttagg tcagacaagg tgtccgggaa agtcaatggg aagagaaaaa tttgttaaaa
    20941 ataactgttc ggcgatttct tgaacggcag aataggttgg ataaattgat atagatggtt
    21001 tattaatctt tttgttaatg attgcaaaaa ttgatctaag cacgagtcat cacataatct
    21061 gatgtatcag ttgtggtcaa tatataagcg aatgtaagcg attgttgatg tcgaaatggt
    21121 tgttttctat tcggttattg ttaaatgcga agccattttg
//
LOCUS       DAZPDU010000063        21007 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZPDU010000063 DAZPDU010000000
VERSION     DAZPDU010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21007
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.064"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..174)
                     /locus_tag="ACHURS_002131"
     CDS             complement(<1..174)
                     /locus_tag="ACHURS_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0758 domain-containing protein"
                     /protein_id="HFR1237857.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGL"
     gene            346..1566
                     /gene="coaBC"
                     /locus_tag="ACHURS_002132"
     CDS             346..1566
                     /gene="coaBC"
                     /locus_tag="ACHURS_002132"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFR1237858.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEVAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            1544..2002
                     /gene="dut"
                     /locus_tag="ACHURS_002133"
     CDS             1544..2002
                     /gene="dut"
                     /locus_tag="ACHURS_002133"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFR1237859.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            2109..2705
                     /gene="slmA"
                     /locus_tag="ACHURS_002134"
     CDS             2109..2705
                     /gene="slmA"
                     /locus_tag="ACHURS_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFR1237860.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(2742..3383)
                     /gene="pyrE"
                     /locus_tag="ACHURS_002135"
     CDS             complement(2742..3383)
                     /gene="pyrE"
                     /locus_tag="ACHURS_002135"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFR1237861.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KNHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RSEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(3449..4165)
                     /gene="rph"
                     /locus_tag="ACHURS_002136"
     CDS             complement(3449..4165)
                     /gene="rph"
                     /locus_tag="ACHURS_002136"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFR1237862.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            4292..5155
                     /locus_tag="ACHURS_002137"
     CDS             4292..5155
                     /locus_tag="ACHURS_002137"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFR1237863.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            5377..6201
                     /gene="dinD"
                     /locus_tag="ACHURS_002138"
     CDS             5377..6201
                     /gene="dinD"
                     /locus_tag="ACHURS_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFR1237864.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSINQLENSVKITEKK"
     gene            6493..7110
                     /locus_tag="ACHURS_002139"
     CDS             6493..7110
                     /locus_tag="ACHURS_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFR1237865.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(7107..8789)
                     /gene="ligB"
                     /locus_tag="ACHURS_002140"
     CDS             complement(7107..8789)
                     /gene="ligB"
                     /locus_tag="ACHURS_002140"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFR1237866.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLAHKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQRLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            9047..9670
                     /gene="gmk"
                     /locus_tag="ACHURS_002141"
     CDS             9047..9670
                     /gene="gmk"
                     /locus_tag="ACHURS_002141"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFR1237867.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            9725..10000
                     /gene="rpoZ"
                     /locus_tag="ACHURS_002142"
     CDS             9725..10000
                     /gene="rpoZ"
                     /locus_tag="ACHURS_002142"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFR1237868.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            10019..12127
                     /gene="spoT"
                     /locus_tag="ACHURS_002143"
     CDS             10019..12127
                     /gene="spoT"
                     /locus_tag="ACHURS_002143"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFR1237869.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            12134..12823
                     /gene="trmH"
                     /locus_tag="ACHURS_002144"
     CDS             12134..12823
                     /gene="trmH"
                     /locus_tag="ACHURS_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFR1237870.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            12829..14910
                     /gene="recG"
                     /locus_tag="ACHURS_002145"
     CDS             12829..14910
                     /gene="recG"
                     /locus_tag="ACHURS_002145"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFR1237871.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(15079..16284)
                     /gene="gltS"
                     /locus_tag="ACHURS_002146"
     CDS             complement(15079..16284)
                     /gene="gltS"
                     /locus_tag="ACHURS_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFR1237872.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            16564..17955
                     /gene="xanP"
                     /locus_tag="ACHURS_002147"
     CDS             16564..17955
                     /gene="xanP"
                     /locus_tag="ACHURS_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFR1237873.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            18076..19785
                     /locus_tag="ACHURS_002148"
     CDS             18076..19785
                     /locus_tag="ACHURS_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFR1237874.1"
                     /translation="MKFIGKLLLYILIALLVAIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMVHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKHQVTQTMNAGVVSSGEVTSAE
                     LVQ"
     gene            complement(19837..>21007)
                     /gene="yicI"
                     /locus_tag="ACHURS_002149"
     CDS             complement(19837..>21007)
                     /gene="yicI"
                     /locus_tag="ACHURS_002149"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFR1237875.1"
                     /translation="LAIYDFTNPDACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQW
                     FDGSDPQKMHNHYAYIYNELVWNVLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYA
                     NYESMAESLRGGLSIGLSGFGFWSHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSK
                     SYRVPWAYDDESCDVVRFFTQLKCRMMPYLYREAARAKARGTPMMRAMMMEFPDDPAC
                     DYLDRQYMLGDNVMVAPVFTEAGEVQFYLPEGRWTHLWHNNELDGSRWHKQQHGFLSL
                     PVYVRDNTLLALGNNDQRPDYAWHEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAT
                     RTGNTITVTGAGEARNWTLCLRNIVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
BASE COUNT         5085 a         5717 c         5386 g         4819 t
ORIGIN      
        1 caagccataa agagagccga aattctccag catctctttt gccagggtta atacatcttt
       61 accgcgcgtt ccggtacgca gaaatagcgc cagcagctcg acatccgtta aggcgctaat
      121 accaaacttc agcatttttt cgcgcggcat caacagctgt gcattgtttt tcaccttcac
      181 ctcctttgtg gtgctcgcat cctgtcatag ccgtttcgtt aaatcgacgg ccagttttca
      241 atcctggaaa gcgcctcgca aagtgaatct caggtgaagg cacgacaaca aaagtattgt
      301 gataaaatcg ccaacttctg gtgtcaccct acaggaaaaa tcatcatgag cctggccggt
      361 aaaaaaatcg ttctcggcgt tagcggcggt attgctgcct ataaaacccc tgaactggtg
      421 cgtcgtttgc gcgatcgcgg ggccgacgtc cgcgtagcca tgaccgaagc ggcaaaagcc
      481 tttatcaccc cacttagctt gcaggcggtt tctggttatc ccgtttccga cagtctgctg
      541 gacccggcag ccgaagccgc tatgggccat attgagctgg gtaaatgggc tgatttagtg
      601 attctcgccc ctgccacggc agatttgatt gcccgtgttg ctgccggaat ggcgaatgac
      661 ctggtatcga cgatttgtct ggctacacct gcgcctgtag ccgtgctccc cgccatgaac
      721 cagcagatgt accgtgccgc tgccacgcag cataatttag aggtgcttgc ttcccgtggt
      781 ttgctcatct gggggccaga cagtggcagt caggcttgtg gtgatatcgg tcctgggcga
      841 atgctcgatc cgttaaccat tgtggatatg gcggtagcgc atttttcgcc cgtcaacgac
      901 ctgaaacatc tgaacattat gattaccgcc ggcccgacgc gtgaaccgct cgatccggtg
      961 cgttatatct ctaatcacag ctccggcaag atgggttttg ctatcgccgc cgccgctgcc
     1021 cgtcgtggcg cgaacgtcac gctggtatca ggcccggttt cgctaccgac gccaccgttt
     1081 gttaaacgtg ttgatgtgat gaccgcgctg gaaatggaag tcgccgtgaa tgcttctgta
     1141 cagcagcaaa atatttttat cggctgcgcc gccgtggcgg attatcgcgc agctaccgtg
     1201 gccccagaga aaatcaaaaa gcaggccacg cagggtgatg aattaacaat aaaaatggtt
     1261 aaaaaccccg atatcgtcgc aggcgttgcc gcactaaaag accatcgacc ctacgtcgtt
     1321 ggatttgccg ccgaaacaaa taatgtggaa gaatacgccc ggcaaaaacg tatccgtaaa
     1381 aaccttgatc tgatctgcgc gaacgatgtt tcccagccaa ctcaaggatt taacagcgac
     1441 aacaacgcat tacacctttt ctggcaggac ggagataaag tcttaccgct tgagcgcaaa
     1501 gagctccttg gccaattatt actcgacgag atcgtgaccc gttatgatga aaaaaatcga
     1561 cgttaagatt ctggacccgc gcgttgggaa ggaatttccg ctcccgactt atgccacctc
     1621 tggctctgcc ggacttgacc tgcgtgcctg tctcgacgac gccgtagaac tggctccggg
     1681 tgacactacg ctggttccga ccgggctggc gattcatatt gccgatcctt cactggcggc
     1741 aatgatgctg ccgcgctccg gattgggaca taagcacggt atcgtgcttg gtaacctggt
     1801 aggattgatc gattctgact atcagggcca gttgatgatt tccgtgtgga accgtggtca
     1861 ggacagcttc accattcaac ctggcgaacg catcgcccag atgatttttg ttccggtagt
     1921 acaggctgaa tttaatctgg tggaagattt cgacgccacc gaccgcggtg aaggcggctt
     1981 tggtcactct ggtcgtcagt aacacatacg catccgaata acgtcataac atagccgcaa
     2041 acatttcgtt tgcggtcata gcgtgggtgc cgcctggcaa gtgcttattt tcaggggtat
     2101 tttgtaacat ggcagaaaaa caaactgcga aaaggaaccg tcgcgaggaa atacttcagt
     2161 ctctggcgct gatgctggaa tccagcgatg gaagccaacg tatcacgacg gcaaaactgg
     2221 ccgcctctgt cggcgtttcc gaagcggcac tgtatcgcca cttccccagt aagacccgca
     2281 tgttcgatag tctgattgag tttatcgaag atagcctgat tactcgcatc aacctgattc
     2341 tgaaagatga gaaagacacc acagcgcgcc tgcgtctgat tgtgttgctg cttctcggtt
     2401 ttggtgagcg taatcctggc ctgacccgca tcctcactgg tcatgcgcta atgtttgaac
     2461 aggatcgcct gcaagggcgc atcaaccagc tgttcgagcg tattgaagcg cagctgcgcc
     2521 aggtattgcg tgaaaagaga atgcgtgagg gtgaaggtta caccaccgat gaaaccctgc
     2581 tggcaagcca gatcctggcc ttctgtgaag gtatgctgtc acgttttgtc cgcagcgaat
     2641 ttaaataccg cccgacggat gattttgacg cccgctggcc gctaattgcc gcgcagttgc
     2701 agtaatatga cgccggatga cttttcatcc ggcgagtttc tttaaacgcc aaactcttcg
     2761 cgataggcct taaccgccgc cagatgttcc gccatttccg gcttctcttc caggtaagca
     2821 atcaggtctt tcagggtgat gatagagatc actttgcagt tgtaatcacg ctcaacttcc
     2881 tgaatcgccg aaatctcgct gcgcccccgt tcctgacgat cgagcgaaat caacacgcca
     2941 gcaagcgtcg cgccattggc ctgaataatt tccatcgact cacgaatcgc cgttccggcg
     3001 gtgatcacat catctaccag cattacgcgt ccttgtaacg cgctaccaac cagattgccg
     3061 ccttcaccgt ggttttttgc ctctttgcgg ttaaagcagt acggcaggtc caggtcatga
     3121 tgctccgcca gcgccacggc ggttgtggtg gcaatcggga tccctttgta agcagggcca
     3181 aacagcagat cgaactcaat gccggaatcc accaatgctt cagcgtaaaa acggcctaac
     3241 agtgccaggt cgcgcccggt attaaacagc ccggcgttga agaaataggg gcttttacgc
     3301 ccggatttca gcgtaaactc gccaaacttt aacacctgct tgctaagcgc aaattcaata
     3361 aactggcgct gatatggttt catgccttcg ctcctcatct tacttttcta cagacaaaaa
     3421 aaaggcgact catcagtcgc cttaaaaatc agtttgccag cgccgccttc tgcgtcgcta
     3481 caatggattc gattccccct cgggccagag ccaacaaggt gagtaattct tcgtgggtga
     3541 acggttcgcc ttctgccgtc ccctgcactt caatgatgcg cccgtcttcg gtcatcacta
     3601 cgttcatgtc ggtctctgcg gcagagtctt caacgtattc cagatcgcaa accgcttcgc
     3661 cgttcacaat cccgacagaa accgcggcta ccatcccttt catcggattg gttttcagct
     3721 taccgttttc caccagcttc tgtagcgcat ctgccagcgc cacgcaggca cccgtaatcg
     3781 aagcggtacg agtgccacca tcagcctgaa gcacgtcgca gtccagcgta atggtgaact
     3841 cacccagcgc tttcaaatct actgccgcgc gaagcgcacg ggcgatcaga cgctggattt
     3901 ccattgtgcg tccaccctgc ttacctttcg ccgcttcacg agcgttacgg gtgtgggtag
     3961 aacgcggcag cataccgtac tctgcggtga tccagccctg gccctgacct ttcaggaagc
     4021 gcggcacgcc ttcttcaata gaggcggtac acaacacttt ggtatcgcca aattcgacca
     4081 gcaccgagcc ttctgcatgt tttgtatagt tacgagtcag ggtaacggga cgcacctgat
     4141 tattgctacg gcctgctgga cgcatattga aatctccggc ttgaaacaaa tgtggctgcg
     4201 cattatacgg acttccggag gttattccta tcctgacaag gcatcgatgg ctataatcct
     4261 tccacctctc cttttataaa caggaacgtc tatgatccgc agtatgaccg cctacgcccg
     4321 gcgtgaaatc aagggtgaat gggggagcgc aacctgggaa atgcgctcgg taaaccagcg
     4381 ttatctggaa acttactttc gtctgccgga gcagttccgt agccttgaac ctgtcgttcg
     4441 cgagcgtatt cgttctcgcc tgacgcgcgg taaagtggaa tgtaccctgc gctatgagcc
     4501 agatgttagc gcgcaaggtg agctgatcct caacgaaaaa ctggctaaac agctggtaac
     4561 tgccgcgaac tgggtaaaaa tgcagagtga cgaaggggaa atcaacccgg ttgatattct
     4621 gcgctggccg ggcgtaatgg cagcccagga gcaggatctc gacgcgattg ccgctgaaat
     4681 tctcgcggcg ttggatggta ccctggacga ctttatcgtc gcacgtgaaa ccgaaggtca
     4741 ggcgctgaaa gcgttgatcg agcagcgtct ggaaggcgtc accgccgaag tggtcaaagt
     4801 ccgcgcccat atgccggaaa tcctgcaatg gcagcgtgag cgtctggtcg cgaagctgga
     4861 agatgctcaa gtgcaactgg aaaacaaccg cctggagcag gagttggttc tgctggcaca
     4921 acgaattgat gttgccgaag aactggaccg cctggaagcg catgtcaaag agacctacaa
     4981 cattctgaag aaaaaagaag ccgttggtcg tcgtctggac tttatgatgc aagagttcaa
     5041 ccgcgagtcg aacactcttg cgtcgaaatc tatcaatgcc gaagtgacga attctgccat
     5101 cgagctgaaa gtgttgattg agcagatgcg cgagcagatt cagaatattg agtaactcgt
     5161 aacactaatt cttacctttc agccttcaca aatgccgccc actcaaacag agcggcattt
     5221 ttcttccccg caacattcaa ttctgttttg cgtacctgct ccagattttg cgatgttttt
     5281 ttgcgcagcg cagtgagaac gtgagatact cacaactgta tataaataca gttatagact
     5341 tactttcttt gcaatcgata tcacatggag tgggcaatga acgaacatca tcaacctttt
     5401 gaagaaatac ggcattatgg cactgaaggg caagaatttt ggtcagcgag agaattggca
     5461 ccgttactgg attatcggga ctggcgcaat tttcaaaagg tactggcccg cgcgactcaa
     5521 gcgtgcgaag ccagcaatca agctgcatct gaccatttcg ttgaaaccac caaaatggtt
     5581 gtattaggtt caggggctca acgagaactt gaagatgttc atctctctcg ctatgcctgt
     5641 tacctggtag tacaaaacgg cgaccctgcg aaaccggtta ttgcggcagg gcaaacttat
     5701 tttgctatcc agacccgacg gcaggagctg gctgacgatg aagctttcag gcaacttcgt
     5761 gaagacgaaa agcgtctttt tctgagaaat gagttgaaag aacataacaa acaattagtt
     5821 gaggcagcgc agcaagctgg ggtagcgaca gctatcgatt tcgccatatt tcagaatcat
     5881 ggttaccggg ggctgtatgg tggattagat cagaaagcca tccatcagcg gaaggggctg
     5941 aaaaagagtc agaagatcct ggatcatatg ggttcaacag aactggcggc taatctcttt
     6001 cgagctaccc aaacagaaga aaaactcaag cgggatggcg taaattcaaa acagcaagct
     6061 aataccacac actttgacgt gggtagcaag gtgaggcaaa ccattcagga acttggcgga
     6121 accatgcctg aggagttgcc gaccccgcag gtcagcatca atcagttgga aaacagtgta
     6181 aaaattacag agaagaaata gcagaaacta aaaactcttt ttcttaattg agatacccga
     6241 tgcgtaagcc aaagtccagg tgcagtgaac acaatggcta aacattgcac ctttcttttc
     6301 cacccctcaa ttttaaccta ttttttctta tgcattttct cagacaagaa gtcagaagaa
     6361 tgcatctaca ctacagaaaa tagcgatttc acataactac aagttatcaa tttcctctca
     6421 ccttaaaaaa tctcaatcgt gacaatacgc acaaatcgct accctgccag acagattttt
     6481 agggagagaa ccatgctgtt acacattttg tatttggttg gcattactgc cgaagccatg
     6541 acgggggcgc tggcggccgg acgacggcgc atggatacat ttggcgtaat tattattgcg
     6601 accgccaccg caattggcgg agggtcagtg cgcgatattc tgctgggcca ctatccgctc
     6661 ggctgggtca aacacccgga atatgtgatt atcgtcgcta ccgccgcagt actcaccact
     6721 atcgttgctc ctgtaatgcc ttacctgcgc aaagtgtttc tggtgctcga tgcgctcgga
     6781 ctggtggtct tttctatcat cggcgcacag gttgcactgg atatggggca cggcccaatt
     6841 attgccgttg tcgcggcggt aaccaccggc gtgtttggcg gcgttttgcg cgatatgttc
     6901 tgtaaacgca tcccactggt attccagaaa gagttgtatg ccggtgtctc atttgcctcc
     6961 gccgtgctgt acatcgcgct gcaacactat gtttctaacc atgatgtggt gattatctcc
     7021 accctggtat tcggcttttt tgcccgctta ctggcgctac gtcttaagct gggattaccg
     7081 gtcttttact acagccacga aggtcactaa ggttcaaaac ctgtgatctg ctgggcagcc
     7141 agccaactgc ccagcttctt gatttgcgca ttttccttcc attcaataac ctgtctggcg
     7201 cgcccagatc cagtccccgg cagccgctgc cagaactgct ccgtgctaaa taaaagttgc
     7261 gaccaggacc gttcatcact ggcattaagc gccgcccggg ttaacggtat tcccattgcc
     7321 atcacccagc gagtaaaagg ctgcttatga gccagattaa actgatgcca taactgcgca
     7381 cttttacttt tcgcgatccc cggcgtgttc tgtaattgct ctggcgttaa taaaagccag
     7441 gaaaagatat gctcaaagcg atgagtctga tgcagcgcgc gccagccggc ctcaccaatg
     7501 ccatccagcc caagaacctg ttttgacccc agccagacta agcgtgaaat gaactgttcc
     7561 tgacaaacat cagaagcaaa gtagcaggtc aacgagttaa agcggttttc tggtggtgtc
     7621 ggttttgtac gttctgcacc gcgccagacc acatcatcaa tgcgaggaat accctgaccg
     7681 gcaaggctga cgaggatctg atcaccaggc gcaatatccc actcctgcca gcgcctgacg
     7741 gaaccaatat tcacccgctg gactttttta tcatccagca tgacaggtgc gagtgacgca
     7801 accaccgata ttttaccgct cttacccacc gcaaactgaa tcgccttcac ttcggcaact
     7861 tgagctacag gttgatattt ccaggccacc aaccattctg cctggcctgg tagccaatgg
     7921 cgggattcgg gttccttagc cgctcgcaca accacgccat cggtgacgaa gggtaatttc
     7981 gctttccacc actcattgcg tacgcgcgca acttcatcag catttttaac cgcacgggta
     8041 tacctctgcg ttagagtaaa acctgcggta gccagctcct ttaaacgatc ggtcattaac
     8101 tgcggtccat ccggccatgc ccagacaaaa acagccagtg agtttagcgt gtcgctattc
     8161 ccctggcgca tcatcaggcc agcaactttt gcgcgggcat ttattccccc catttgttgt
     8221 tggatatgcc cctcgcgctg gagaaatatt tccccctgaa gcgtactgtt ggctaaaggc
     8281 ccgctaacgg tttgcggcac agcggaaatt aagctaactt tctgcgtcca gtcctcgcct
     8341 ttcaggccat taccgcgact gattgctttg ttcagtttcc cgtcccgata aaccagggtt
     8401 accgcaacgc catcaacttt tggctgcacc caaagatcgc tacgttctcg catccacaga
     8461 cttaatgcgt ttttatccac cattttgcgt acacccgtat gggcaaccgg atgcataacc
     8521 gcaccattta aaggcggcat catcacgtcg cggggctcgc tcccaaagca gcgttgccac
     8581 tgcgtaagac gggcgcttaa ctgatcgtaa acaccgtctt ccacctcact tttcccttcc
     8641 ttccagtagt catcgtccca ctgttttatt tgctgttgca ggcgggaaat ttcttcctgt
     8701 gctctggctg gcgaccaggc cggacagacc gcccacacag atgattgcca gcacaagata
     8761 cttattaata tcgccatcca tactttcatc atcacctccg ctgtagatag tcaggcagat
     8821 atacaacgtg atgaaagcaa agccgagtgg caaaaacgga gtctgcgagg acgcttcctg
     8881 aaaatcgtct ttattgcaat gaatgacagg caaatgcgga agcagctacg caaaacgcaa
     8941 caactttgcg caaaaagtgt gagcaagggc tacgtcacat ggccgcgccg tgtataataa
     9001 gctcgtatgt aggctttatt tcgctaatca catacgaaag atactcatgg ctcaaggcac
     9061 gctttatatt gtttctgccc ccagtggtgc gggtaaatcc agcctgattc aggctttatt
     9121 aaaaacccaa ccgttgtatg acacccaggt ttctgtttca cacaccacac gccaaccgcg
     9181 tcctggtgaa gtccacggtg aacattattt ctttgttaat catgatgaat ttaaagaaat
     9241 gattagcaga gatgcgttcc tcgaacacgc agaagttttt ggtaattact atggcacttc
     9301 gcgtgaggcc attgagcaag tactggcgac cggtgtcgat gtttttctcg atatcgactg
     9361 gcagggcgcg cagcaaattc gccagaagat gccgcacgcg cggagtatct ttattttacc
     9421 gccgtccaaa attgaactgg accgccgtct acgcggtcgc ggtcaggaca gcgaagaggt
     9481 cattgcaaag cgtatggcgc aagctgttgc agaaatgagc cattacgccg aatatgatta
     9541 tctgattgtg aatgatgact tcgataccgc gttgaccgat ttgaagacca ttattcgcgc
     9601 cgaacgtctg cgcatgagcc gccaaaagca gcgtcatgac gctttaatca gcaaattgtt
     9661 ggcagactga acctgatttc agtatcatgc ccagtcattt cttcacctgt ggagcttttt
     9721 aagtatggca cgcgtaactg ttcaggacgc tgtagagaaa attggtaacc gttttgacct
     9781 ggtactggtc gccgcgcgtc gcgctcgtca gatgcaggta ggcggaaagg atccgctggt
     9841 accggaagaa aacgataaaa ccactgtaat cgcgctgcgc gaaatcgaag aaggtctgat
     9901 caacaaccag atcctcgacg ttcgcgaacg ccaggaacag caagagcagg aagccgctga
     9961 attacaagcc gttaccgcta ttgctgaagg tcgtcgttaa tcacaaagcg ggtcgccctt
    10021 gtatctgttt gaaagcctga atcaactgat tcaaacctac ctgccggaag accaaatcaa
    10081 gcgtctgcgg caggcgtatc tcgttgcacg tgatgctcac gaggggcaaa cacgttcaag
    10141 cggtgaaccc tacatcacgc acccggtagc ggttgcctgc attctggccg agatgaaact
    10201 cgactatgaa acgctgatgg cggcgctgct gcatgacgtg attgaagata cacccgccac
    10261 ctaccaggat atggaacagc tttttggtaa aagcgtcgcc gagctggtag agggggtatc
    10321 gaaacttgat aaactcaagt tccgcgataa gaaagaggcg caggccgaaa actttcgcaa
    10381 gatgattatg gcgatggtgc aggatatccg cgtcatcctc atcaaacttg ccgaccgtac
    10441 ccacaacatg cgcacgctgg gctcacttcg cccggacaaa cgtcgccgca tcgcccgtga
    10501 aactctcgaa atttacagcc cgctggcgca ccgtttaggt atccaccaca ttaaaaccga
    10561 actcgaagag ctgggttttg aggcgctgta tcccaaccgt tatcgcgtaa tcaaagaagt
    10621 ggtgaaagcc gcgcgcggca accgtaaaga gatgatccag aagattcttt ctgaaatcga
    10681 agggcgtttg caggaagcgg gaataccgtg ccgcgtcagt ggtcgcgaaa agcatcttta
    10741 ttcgatttac tgcaaaatgg tgctcaaaga gcagcgtttt cactcaatca tggacatcta
    10801 cgctttccgc gtgatcgtca atgattccga cacctgttat cgcgtgctcg ggcaaatgca
    10861 tagcctgtac aagccgcgtc cgggtcgcgt gaaagactac atcgccattc caaaagcgaa
    10921 cggctatcag tctttgcaca cctcgatgat cggcccgcac ggtgtgccgg ttgaggtcca
    10981 gatccgtact gaagatatgg atcagatggc ggagatgggt gttgccgcgc actgggctta
    11041 taaagagcac ggcgaaacca gcaccaccgc gcaaatccgc gcccagcgct ggatgcaaag
    11101 cctgctggag ctgcaacaga gcgccggtag ttcgtttgaa tttatcgaga gcgttaaatc
    11161 cgatctcttc ccggatgaga tttacgtttt cacaccggaa gggcgaattg tcgagctgcc
    11221 tgccggtgca acgcccgtcg acttcgctta tgcagtgcat accgatatcg gtcatgcctg
    11281 cgtgggcgca cgcgttgacc gccagcctta cccgctgtcg cagccgctta ccagcggtca
    11341 aaccgttgaa atcattaccg caccgggcgc tcgcccgaat gccgcgtggc tgaactttgt
    11401 cgttagctca aaagcgcgcg ccaaaattcg tcagttgctg aaaaacctca agcgtgatga
    11461 ttctgtaagc ctgggccgtc gtctgctcaa ccatgctttg ggtggtagcc gtaagctgaa
    11521 tgaaatcccg caggaaaata ttcagcgcga gctggatcgc atgaagctgg caacgcttga
    11581 cgatctgctg gcagaaatcg gacttggtaa cgcaatgagc gtggtggtcg cgaaaaatct
    11641 gcaacatggg gacgcctcca ttccaccggc aacccaaagc cacggacatc tgcccattaa
    11701 aggtgccgat ggcgtgctga tcacctttgc gaaatgctgc cgccctattc ctggcgaccc
    11761 gattatcgcc cacgtcagcc ccggtaaagg tctggtgatc caccatgaat cctgccgtaa
    11821 tatccgtggc taccagaaag agccagagaa gtttatggct gtggaatggg ataaagagac
    11881 ggcgcaggag ttcatcaccg aaatcaaggt ggagatgttc aatcatcagg gcgcgctggc
    11941 aaacctgacg gcggcaatta acaccacgac ttcgaatatt caaagtttga atacggaaga
    12001 gaaagatggt cgcgtctaca gcgcctttat tcgtctgacc gcccgtgacc gtgtgcatct
    12061 ggcgaatatc atgcgcaaaa tccgcgtgat gccagacgtg attaaagtca cccgaaaccg
    12121 aaattaatgt tttatgaacc caacacgtta tgcacgcatc tgcgaaatgc tcgccaggcg
    12181 gcagcctgat ctgaccgtct gcatggagca ggtccacaaa cctcataacg tttctgcgat
    12241 tattcgtacc gcagatgccg ttggcgtaca tgaagttcac gccgtctggc ctggtagccg
    12301 catgcgcacc atggcttcgg cagcggcggg tagtaacagc tgggtacagg tgaaaacaca
    12361 ccgcaccatt ggcgatgccg tcgctcatct gaaaggccag ggcatgcaga ttctggcaac
    12421 ccatctttct gataacgctg tcgatttccg cgaaattgat tacactcgcc cgacctgcat
    12481 tttgatggga caggagaaaa cgggcatcac gcaggaagca ttggccctgg cggatcagga
    12541 catcatcatt ccgatgatcg gcatggtgca gtcgctgaat gtttccgttg cctcagccct
    12601 cattctttac gaagcccagc gtcagcggca aaatgcaggc atgtacctgc gtgaaaacag
    12661 catgttgccg gaagcagagc aacaacgcct gttgtttgaa ggcggctatc cggtgctggc
    12721 gaaagtcgca aaacgcaaag gcctgcctta tccccacgtc aatcagcaag gcgagatcga
    12781 agctgatgcc gactggtggg ctactatgca ggctgcaggg taagtgccat gaaaggtcgc
    12841 ctgttagatg ctgtcccact cagttcccta acgggcgttg gcgcagcact tagtaacaaa
    12901 ctggcgaaaa tcaacctgca taccgtgcag gatctactct tacaccttcc cctgcgctac
    12961 gaagatcgca cccatctcta ccccatcgga gaactactgc cgggcgttta tgccacggtg
    13021 gaaggcgaag tgctgaactg caatatctcc ttcggcggtc ggcggatgat gacctgccag
    13081 atcagcgacg gttccggcat cctcaccatg cgctttttca atttcagcgc ggcaatgaaa
    13141 aatagcctgg cgacgggccg ccgtgtactg gcttatggcg aagcaaagcg cggtaaatat
    13201 ggtgcggaga tgatccaccc ggaataccgc gtgcagggcg atctcagcac gccagaatta
    13261 caggaaacgc tcacgccggt ttatccaaca acggaaggcg taaagcaggc cacgctgcgt
    13321 aaattaaccg accaggcgct ggatctgctc gacacctgcg ccattgaaga actcctgccg
    13381 ccggaactgt cacaaggaat gatgacgcta ccggaagcgt tgcgcacttt gcaccgcccg
    13441 ccaccgacgc tacagcttag cgatctggaa accgggcagc atccggcgca acgtcgtctg
    13501 attctggaag aactgctggc gcacaacctc agcatgttag ccttacgtgc cggagcacag
    13561 cgttttcatg cccagccgct gagcgccaat gatgcgctga aaaataaact cctcgcagcc
    13621 ttaccgttca agccaacggg cgcacaggca cgcgtagtgg cggagatcga gcgcgatatg
    13681 gcgctggatg tgccgatgat gcgcctggta cagggcgatg taggttccgg taaaacgctg
    13741 gtcgccgccc tcgccgcgct gcgtgcgatt gcccacggca aacaggtggc attgatggca
    13801 ccaaccgaat tactcgccga acagcacgcc aataacttcc gcaactggtt tgaaccgctc
    13861 ggtatcgaag tgggctggct cgccggtaag cagaaaggca aagcgcgact gtctcagcag
    13921 gaagccatcg ccagcggtca ggtgcagatg attgttggta cgcacgccat cttccaggaa
    13981 caggtgcagt ttaacggtct ggcgctggtg attatcgacg aacaacatcg ctttggcgtg
    14041 catcagcgtc tggcgttatg ggagaaaggc cagcagcaag gcttccatcc gcatcagttg
    14101 atcatgaccg ccacgccgat cccccgcacg ctggcaatga cggcgtatgc cgatctcgat
    14161 acctcggtga tagatgaact gccgccaggc cgcacgccag tgactacggt cgctattcct
    14221 gatactcgcc gtaccgacat cattgaccgc gtgcgccacg cctgcatgac tgaaggtcgt
    14281 caggcgtact gggtttgtac gttgattgaa gagtcagagt tactggaagc gcaggcggcg
    14341 gaagctacct gggaagagtt gaaactggcg ctaccagaat tgaatgttgg cctggtacac
    14401 gggcggatga aaccagccga gaaacaggcg gtgatggcgt cgtttaaaca aggtgagcta
    14461 catctgctgg ttgccactac cgttattgaa gtcggtgttg atgtgcctaa cgccagcctg
    14521 atgattatcg aaaaccctga gcgtctgggt ctggcgcagt tacaccagct gcgcgggcgc
    14581 gtaggtcgtg gcgcggtggc ttctcactgc gtgctactct acaaaacgcc gctttctaaa
    14641 acggcgcaaa ttcgcctgca agtgctgcgc gacagtaacg acggttttgt gattgcgcaa
    14701 aaagatctgg agattcgcgg ccctggcgaa ttgttaggca cgcgtcagac gggtaatgct
    14761 gaatttaaag tggcggattt actgcgcgat caggcgatga tcccggaagt tcagcgcctg
    14821 gcacgccata ttcacgaacg ttacccacaa caggcaaaag ccctgataga acgctggatg
    14881 ccggagacgg aacgttactc gaatgcgtaa aaggcggcag tgagaagacc gccatttcag
    14941 gttaccctac cttcctgccg gatgcgattc atcaccctac aaattcaata aattatgaat
    15001 caatacgcag gcttgataag cgaagcgtat caggcatttg tctttgttgc ggcggcactt
    15061 ctaccgccgc ttcatcggtt aaccggcaaa aatcggcaac atcaaataca acttaattac
    15121 cagcgcattg acgatatcga taaagaacgc accgaccatc ggcaccacca aaaacgccat
    15181 gtgcgacggg ccaaagcgtt cagtaatcgc ctgcatgttg gcgattgccg ttggcgttgc
    15241 accgaggcca aaaccacagt gacctgcagc cagcactgcc gcatcgtagt ttttgcccat
    15301 catgcgccag gtaacgaaga tggcatacaa cgccatgaag atggtctgta ccaccagaat
    15361 cgccagcatc ggcagcgcca gcgaagccag ctcccacagt ttcagcccca tcaacgccat
    15421 cgccaggaac aacgacaagc ttacgttacc cagcacggat accgcacgct caaagacgcg
    15481 gtaaaagccc attattgaca gaccgttgct cagaatcacg ccaacaaaca gtacacagac
    15541 gaaggtcggc agttcaaaag cagtgccagc caaaagttgc gcaacaattt tccccaccgt
    15601 caggcagata gcaatcagcg cgatagtttc aatcagcacc aacgaggtga tcatgcgtcc
    15661 cacatccggc ttttcaaacg ccgtcgggac ttcctggtca tccggaatac cgttcggcgt
    15721 ggtggagtgt ttcaccagat agcgcgccac cggaccgcca atcaagccgc ccagcaccag
    15781 accgaacgtt gcacaggcca tcgccacttc cgtcgcattg gtgaagccat aacgttcaat
    15841 gaacaattta ctccacgcag cgcccgtacc gtgaccgccg gaaagcgtaa tagaaccggc
    15901 caacagcccc atcagcggat caagccctaa caggctagcc ataccaatgc caatggcatt
    15961 ttgcatcacc aacagaccaa caaccacaat caagaagatg ccaaccacac gcccaccggc
    16021 acgcaaactg gcaatgttgg cgttcaggcc aatggtggcg aagaaagcca gcattaacgg
    16081 atcgcgcagg gacatatcaa agttgacttc ccagcccatg ctttttttca gtactagtag
    16141 cgccagcgcc accaacaaac cacccgcaac aggttccggt atggtgtatt tcttcaaaaa
    16201 ggagacggaa tggaccaact tacgcccgag cagcaacgtc agcgttgcgg caacaagcgt
    16261 tgctaaagta tcgagatgaa acatagttac tcctttgtat ccgcatcact ctttcatact
    16321 cgtcatactt catcgcgcag tggcaacttc atgaactatg acgggtatcg agttatgctt
    16381 tcctgggtca aattctcagc tgttaatgaa caaatggcat ggattttaag cagaaaaata
    16441 ccaaaagtta tataaaaaag tgcatttatc tcatttaaat atttagatga ggatgtggca
    16501 aacgtttgct tttctctttc ggtcagctaa aatgcccgct ttgctaccac gggattgttt
    16561 tcgatgtctg tttccaccct cgagtcagaa aatgcgcaac cggttgcgca gactcaaaac
    16621 agcgaactga tttaccgtct tgaagatcgt ccgccgcttc ctcaaaccct gtttgccgcc
    16681 tgtcagcatc tgctggcgat gttcgttgcg gtgatcacgc cagcgctatt aatctgccag
    16741 gcgctgggtt taccggcaca agacacgcaa cacattatta gtatgtcgct gtttgcctcc
    16801 ggtgtggcat cgattattca aattaaggcc tggggtccgg ttggctccgg gctgttgtct
    16861 attcagggca ccagcttcaa ctttgttgcc ccgctgatta tgggcggtac cgcgctgaaa
    16921 accggtggtg ctgatgttcc taccatgatg gcggctttgt tcggcacgtt gatgctggca
    16981 agttgcaccg agatggtgat ctcccgcgtt ctgcatctgg cgcgccgcat tattacgccg
    17041 ctggtttctg gcgttgtggt gatgattatc ggcctgtcgc taattcaggt ggggctgacc
    17101 tccattggcg gcggttacgc agccatgagc gataacacct tcggcgcacc gaaaaatctg
    17161 ctgctggcag gcgtggtttt agccttaatt atcctgctta accgtcaacg taacccttac
    17221 ttacgcgtgg cctcactggt aattgcgatg gcggccggat atgcgctggc gtggtttatg
    17281 ggcatgttgc cagaaagcaa cgaaccgatg acgcaagaac tgattatggt gccaacgccg
    17341 ctctattacg gtcttggcat tgaatggagt ctgctgctgc cgctgatgct ggtctttatg
    17401 atcacttcgc tggaaaccat tggcgatatc acggcgacct ctgacgtttc cgaacagcca
    17461 gtgtccggtc cgctgtacat gaaacgcctg aaaggcggcg tgctggcaaa cggcctgaac
    17521 tcgtttgttt cggcggtgtt taacaccttc ccgaactcct gcttcgggca aaacaacgga
    17581 gtgatccagt tgaccggtgt tgccagccgc tatgtcggtt ttgtcgtcgc gctgatgttg
    17641 atcgtgctgg gtctgttccc ggcagtgagc ggttttgtac aacacattcc agaaccggtt
    17701 ctgggcggcg caacgcttgt aatgtttggc accatcgccg cctccggtgt gcgtatcgtt
    17761 tctcgtgagc cgctgaaccg tcgggcgatt ctgattatcg cgctgtcgct ggcggttggt
    17821 ctgggcgtgt ctcagcagcc gctgattttg cagtttgccc ctgaatggct gaaaaacctg
    17881 ctctcctccg ggatcgccgc gggcggtatt actgccatcg tgctgaatct gattttccca
    17941 ccagaaaaac agtaattcac tggcggcggt aagataatta ccgccgcagt ctcacctctt
    18001 taaccattcc cgccttgagg attgcgcgta aatcgtgcat aactccctta tgtgcatttc
    18061 acgggatgga agaccatgaa atttattggg aagctgcttc tctacattct catcgctctg
    18121 ttagtggcga tcgctggcct ctattttctt ctgcaaaccc gctggggagc agaacatatc
    18181 agcgcatggg tttccgagaa tagcgactat catctggcct tcggggcgat ggttcaccgt
    18241 ttttccgcgc catctcatat cgtgctggag aatgtcacgt ttggtcgtga tggccagccc
    18301 gcgaccctgg tggccaaaag tgtcgacatt gcgctaagta gtcggcaact gaccgaacca
    18361 cgccatgtcg ataccatcct gctggaaaac gggacgctga atctcaccga ccagaccgcg
    18421 ccgctaccgt tcaaagccga tcgtctgcaa ctgcgtgata tggcgtttaa tagcccgaat
    18481 agcgaatgga aactgagcgc gcagcgggta aatggcggcg tggttccgtg gtcaccagaa
    18541 gccggtaaag tgctgggtac gaaggcgcag attcagttta gtgccggatc gctttcgctc
    18601 aatgatgttc ctgccaccaa tgtactgatt gaaggcagta ttgataacga tcgcgttacg
    18661 ctgactaacc tgggtgccga catcgcgcgc gggacattaa ccggaaacgc gcagcgtaac
    18721 gccgacggca gctggcaagt ggaaaacctg cgcatggcgg atatccgtct acaaagcgaa
    18781 aaatcgctaa ccgacttctt tgcgccatta cgctctgtcc cgtcgttgca gattggtcgc
    18841 ctggaagtga tcgatgctcg tttgcaaggt ccggactggg cggtgaccga cctcgatctc
    18901 agcttgcgca acatgacctt cagtaaagat gactggcaga cacaagaagg caaactgtcg
    18961 atgaacgcta gcgagttcat ttatggttcg ctgcatttat ttgacccgat tataaacgcg
    19021 gaattttctc cgcagggcgt agcgctgcgc cagttcacca gccgctggga agggggtatg
    19081 gtcagaacgt cagggaactg gctgcgtgac gggaaaacgt tgatccttga tgatgcggca
    19141 attgccggac tggaatatac cttgccgaaa aactggcaac agttgtggat ggaaacgaca
    19201 cccggttggt taaacagcct gcaactgaag agatttagcg ccagccgcaa tctgatcatt
    19261 gatatcgacc ctgacttccc gtggcagctc accgcgctcg atggttacgg tgccaacctg
    19321 acgctggtta ccgatcataa atggggcgtc tggagtggct cggcgaatct gaatgccgcc
    19381 gccgcgacgt ttaatcgtgt tgatgtccgt cgtccgtcgc tggcgctaac cgccaacagc
    19441 agcacggtga atatcagcga actgagtgca tttactgaaa aaggcattct ggaagccacc
    19501 gccagtgttt cacaaacgcc acaacgtcag acacatatca gcctgaatgg acgcggtgtg
    19561 ccggtgaata ttttgcaaca gtggggatgg cctgaattac cgttgactgg cgacggcaat
    19621 attcagctta ccgccagtgg cgatattcag gccaatgtcc cgttgaaacc tacggttagc
    19681 gggcaattgc atgccgtgaa cgccgcaaag caccaagtga ctcaaaccat gaatgcgggc
    19741 gtcgtttcca gcggtgaagt tacatcggca gagctggtgc agtaaacatc aatctcgccg
    19801 ggtacgactc agacgtaccc ggcattcttc aatagattac aacgtaattg tcagcgcatt
    19861 cccttgaggc gtaaccacca gcccctgctc actttcagcc tgcgaaccgc cttgcagacc
    19921 atttactttc acaatattgc gcaagcacag tgtccagttc ctcgcctcgc ccgcaccagt
    19981 cacagtaatc gtgttaccag tacgtgttgc ttttagagta aagatcaccg aaccgtcagc
    20041 agcgggcact tcacatacag cttcatgccc gtcttgcaga ttaaagagat ggaatgccgt
    20101 gccttcgtgc cacgcgtaat cgggacgttg atcgttgttg cccagcgcca atagggtgtt
    20161 atcacgcaca taaacgggca gactcaggaa gccgtgctgc tgtttatgcc agcgactacc
    20221 gtcgagctca ttgttgtgcc acaggtgtgt ccagcgacct tccggcaagt agaactgcac
    20281 ctcgcccgcc tcagtgaaca ccggagcaac catcacgttg tcgcctagca tgtattgacg
    20341 gtcaaggtaa tcacaagccg gatcgtccgg gaactccatc atcatggccc gcatcatcgg
    20401 cgtaccccgc gctttcgcac gcgcagcttc acgatacaga tacggcatca tgcggcattt
    20461 cagttgcgtg aagaagcgca ccacatcaca ggactcatca tcgtaggccc acggcacacg
    20521 ataagatttg ctgccatgta aacgactatg gctggagagc aaaccaaacg cgcaccagcg
    20581 tttgtaaacg tgtgccggcg cggtattttc aaagccgccg atatcgtggc tccagaagcc
    20641 aaaacctgaa aggccaatag acaaaccacc gcgcaggctt tccgccattg attcgtagtt
    20701 agcgtaacaa tcgccacccc agtgtaccgg gaatttctgc gcaccgacgg aggccgagcg
    20761 ggcaaacaag acagcttctt cctcaccaac ggtgtccttg agcacgttcc acaccagttc
    20821 gttgtagatg tacgcataat ggttatgcat tttctgcgga tcggaaccgt caaaccactg
    20881 aacatcagtt gggatacgtt cgccaaagtc ggtcttaaag caatcaacgc ccatcgcgac
    20941 cagacctttc agtttgtcgg cgtaccattt gcaggcatcc ggattggtaa agtcataaat
    21001 cgccaga
//
LOCUS       DAZPDU010000064        20901 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZPDU010000064 DAZPDU010000000
VERSION     DAZPDU010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20901
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.065"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(93..1952)
                     /gene="gss"
                     /locus_tag="ACHURS_002150"
     CDS             complement(93..1952)
                     /gene="gss"
                     /locus_tag="ACHURS_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFR1237876.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2157..3023
                     /gene="yghU"
                     /locus_tag="ACHURS_002151"
     CDS             2157..3023
                     /gene="yghU"
                     /locus_tag="ACHURS_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFR1237877.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3052..3133)
                     /locus_tag="ACHURS_002152"
                     /pseudo
     CDS             complement(3052..3133)
                     /locus_tag="ACHURS_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3146..3394)
                     /gene="hybG"
                     /locus_tag="ACHURS_002153"
     CDS             complement(3146..3394)
                     /gene="hybG"
                     /locus_tag="ACHURS_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFR1237878.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3407..3748)
                     /gene="hypA"
                     /locus_tag="ACHURS_002154"
     CDS             complement(3407..3748)
                     /gene="hypA"
                     /locus_tag="ACHURS_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFR1237879.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3741..4229)
                     /gene="hybE"
                     /locus_tag="ACHURS_002155"
     CDS             complement(3741..4229)
                     /gene="hybE"
                     /locus_tag="ACHURS_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFR1237880.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4222..4716)
                     /locus_tag="ACHURS_002156"
     CDS             complement(4222..4716)
                     /locus_tag="ACHURS_002156"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFR1237881.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4716..6419)
                     /gene="hybC"
                     /locus_tag="ACHURS_002157"
     CDS             complement(4716..6419)
                     /gene="hybC"
                     /locus_tag="ACHURS_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFR1237882.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYGGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6416..7594)
                     /gene="hybB"
                     /locus_tag="ACHURS_002158"
     CDS             complement(6416..7594)
                     /gene="hybB"
                     /locus_tag="ACHURS_002158"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFR1237883.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7584..8570)
                     /gene="hybA"
                     /locus_tag="ACHURS_002159"
     CDS             complement(7584..8570)
                     /gene="hybA"
                     /locus_tag="ACHURS_002159"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFR1237884.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8573..9691)
                     /gene="hybO"
                     /locus_tag="ACHURS_002160"
     CDS             complement(8573..9691)
                     /gene="hybO"
                     /locus_tag="ACHURS_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFR1237885.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9880..10167)
                     /gene="yghW"
                     /locus_tag="ACHURS_002161"
     CDS             complement(9880..10167)
                     /gene="yghW"
                     /locus_tag="ACHURS_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFR1237886.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10286..11173)
                     /gene="yghX"
                     /locus_tag="ACHURS_002162"
     CDS             complement(10286..11173)
                     /gene="yghX"
                     /locus_tag="ACHURS_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFR1237887.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYIMYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11330..12370
                     /gene="gpr"
                     /locus_tag="ACHURS_002163"
     CDS             11330..12370
                     /gene="gpr"
                     /locus_tag="ACHURS_002163"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFR1237888.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12410..12904)
                     /locus_tag="ACHURS_002164"
     CDS             complement(12410..12904)
                     /locus_tag="ACHURS_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFR1237889.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13095..13979
                     /gene="yghA"
                     /locus_tag="ACHURS_002165"
     CDS             13095..13979
                     /gene="yghA"
                     /locus_tag="ACHURS_002165"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFR1237890.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(14139..14564)
                     /gene="exbD"
                     /locus_tag="ACHURS_002166"
     CDS             complement(14139..14564)
                     /gene="exbD"
                     /locus_tag="ACHURS_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFR1237891.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14571..15305)
                     /gene="exbB"
                     /locus_tag="ACHURS_002167"
     CDS             complement(14571..15305)
                     /gene="exbB"
                     /locus_tag="ACHURS_002167"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFR1237892.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15557..16744
                     /gene="metC"
                     /locus_tag="ACHURS_002168"
     CDS             15557..16744
                     /gene="metC"
                     /locus_tag="ACHURS_002168"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFR1237893.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16884..17543
                     /gene="yghB"
                     /locus_tag="ACHURS_002169"
     CDS             16884..17543
                     /gene="yghB"
                     /locus_tag="ACHURS_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFR1237894.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17583..18482)
                     /gene="yqhC"
                     /locus_tag="ACHURS_002170"
     CDS             complement(17583..18482)
                     /gene="yqhC"
                     /locus_tag="ACHURS_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFR1237895.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVDYESALQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18676..19839
                     /gene="yqhD"
                     /locus_tag="ACHURS_002171"
     CDS             18676..19839
                     /gene="yqhD"
                     /locus_tag="ACHURS_002171"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFR1237896.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKI
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            19944..20771
                     /gene="dkgA"
                     /locus_tag="ACHURS_002172"
     CDS             19944..20771
                     /gene="dkgA"
                     /locus_tag="ACHURS_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFR1237897.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKAPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
BASE COUNT         5152 a         5617 c         5234 g         4898 t
ORIGIN      
        1 aataaatatt agaacatata aatattgaat attttgatta atccccgtac tgattattct
       61 tcataatcag tacggggttg ctacaacatg tattactttt tcaccacaat taacggttca
      121 atatcactct cttttttgat gaccagtgat tcatcaccgc gcaaacacgt cccaccgtag
      181 ttgccgccca cggtgaaggt acatacctga atgtatttac cgtccacttt cggcaaacac
      241 cacagttgct gatagatgtt tttctgctcg gcaaatttac cgctggtttt gtccagcacc
      301 tcttcatgat ggctgaccag gtcgatattg ctaccacagc gaccggcaat cggtttcact
      361 gcgtaacctg ttttcaccaa ttcatcatta acagtgaaat cggtatccag caggtaacga
      421 tggtgcggga acagcgacca gaggatcggc agaatcgctt tgttgccggg gatcaccgtc
      481 cacagcggct caaagactag cacttccggg cgcagcaata cgtcgataag acgcacttcg
      541 ttttgcggat gaccggtacg gattggcacc gcagcaaact cacggtcgct aacttcacga
      601 atctgatcaa acgcggtttc ccacgcccag gttttccaca cgcagttaac cagtcgccct
      661 tccccatcaa tcagttgccc ggcagcatcc cagcccagtt catccaatcc acgcaagata
      721 cgcgtttcaa agcccgcctg gtgcagcgcc tgctccataa actgcgcgtg atagttttcc
      781 tcgatatctt tgtcctgcat gatatggaca aacggacgtg cacgactgtg tttccaggca
      841 ccagccaatt cgttaatcag cccttccgcc ggattgaagc cgttgccttt atagccctgc
      901 tccgcccaac gttcgaggat caagcccgct tcggtatgac aggaggcgga gtcggcgttg
      961 tactcgtaaa ccttcaggcc acgctcatcc atgcagaaat ccatacgacc agtgatcata
     1021 tggtgacggc gacgctgcca ggagagacgc aaacgtggcc agaggatttt cgggatgtcg
     1081 aacagcgcca gcaggttgtc atctttcagc accttgtcgg ttgcgtgaag atacatcagg
     1141 tgcagctcgt tggtggcttt aattagctcc tgctcggcac tctcggtaat ggtgtagtaa
     1201 tgataaggat cctgattgat cacctgaccg ttggcctgca cataggcgtt ttgcagcgga
     1261 tctttttcat ccagccattt accgtcaaac tggcctttgt tttccaggcg cgctccgctg
     1321 attttcagca gctcgcctgc aatttccggc tgcggtaagc tgtattcagt atcttccgtc
     1381 tggatcatcc agcccagaat ggtggtgtca tcaaaagtgt ctttcagggt atagcagccg
     1441 ttttcgacca ccatctccag ctcacgcgtc cactgttgcc cttgcggcaa cggggaatga
     1501 atcacgttct gttccgcaat acggactttg ttgccatgca attgggtaat gatggcgaca
     1561 tggccagtgt ctttaaattc accgccttta tcccagataa gaagcgcacc cgcgaccggc
     1621 gcacgcggcg agccgttagg aaatgcctgc aatggcagga tgttgtcatt aaccacttct
     1681 cgcaggaagc gcagcgagaa aatctcccac gccataccca cgtcagtaaa gaccacaccg
     1741 taattcagaa agagaaaacg gcgagcaaat tcaacgcatt gccacttgtg gcccatatat
     1801 tcgtcgtcga tatagctacg gaatacggcg tcatcttcgt attcctgcgg atcgagagaa
     1861 ctgtaatctg aagagtagat tgctacccca cctggggcgt agcccaataa tgtcccgaac
     1921 ggggcatcct ggctggtcgt tcctttgctc atcactttac ctttaacaat acaacctaag
     1981 cagttggcgt aattgttgtg ctctgattac ctgctcagcg acattaaccg gacagaggtc
     2041 agactaatca tacacctcat cgcagcggct ggcgcaaagg gttaaaaaat tcacattctg
     2101 tacagcaagt gacctgctac actgcttcaa cactaccact cagaaggcaa ctcactatga
     2161 cagacaatac ttatcagccc gcgaaagtct ggacgtggga taaatccgct ggcggcgcgt
     2221 tcgccaatat caatcgcccg gtttctggtc cgacgcatga aaaaacgctg cccgttggca
     2281 aacacccatt gcaactttat tcgctgggaa cgccgaacgg tcagaaagta acgattatgc
     2341 ttgaggagct gctggcgctg ggcgttactg gtgcagagta cgacgcctgg ctgattcgta
     2401 ttggcgatgg cgatcaattc tccagcggct ttgtcgaagt gaacccaaac tcgaagatcc
     2461 cggcgctgcg cgatcatacg cataatccgc cgatccgcgt gtttgaatct ggttcgatcc
     2521 tgctttatct ggcggagaaa tttggctact tcctgccgca ggatttggca aagcgtactg
     2581 aaacgatgaa ctggctgttc tggttacagg gcgcggcacc gttcctcggc ggtggttttg
     2641 gtcactttta ccattacgcg ccgatgaaaa ttgagtacgc catcaaccgc tttaccatgg
     2701 aagccaaacg tctgctcgac gtgctggata agcaactggc gcagcataag tttgttgcgg
     2761 gcgatgagta caccattgcg gatatggcga tttggccgtg gtttggcaat gtggtgttag
     2821 gcggcgtgta tgatgccgca gagtttcttg atgcgggcag ttataagcat gtacaacgct
     2881 gggcgaaaga agtaggcgaa cgtccggcgg tgaaacgtgg gcgtattgtt aaccgcacca
     2941 acggaccgct gaatgagcag ttgcatgagc gccatgacgc cagtgatttc gagacgaata
     3001 cggaagataa gcgtcagggg taagggttgg tgttcgtcgc agcaagccat ccaggccgga
     3061 taaggcgttc acgccgcatc cggcaatcgt gcacaatgcc tgatgcgacg ctgccgcgtc
     3121 ttatcaggcc tacgaaagct aatcatcacg cgctggtaat gtcgtaatcc atttggcgca
     3181 gtgcgtctaa tgtggctttg gcttcatctt cgtcgatgat gctcatggca aatccgacgt
     3241 gtaccagcac ccactggccc agtagatcgg cagggttacc ttcacaaatc agggcgatat
     3301 tcacgtcgcg cttgatacca catacttcaa cctgcgcaag ctggtgaata tcttcaccga
     3361 cagccagcac ctggcctgga acgccaatac acattattaa ctccggttat tcaacttcaa
     3421 tacttttgac gatcagcgaa tcgccggtat cgacacgcaa ccgctcgccg tgacagagcg
     3481 gacactgcgc atcgtgctga tgaatctcca ccacctggct gcaatcccag caccaagcct
     3541 gggcgggttt atagacgata tgtaaatcgc acccttgcgc caccgttccg tggcagacaa
     3601 tttcaaaact aaaacggacg gcgctctcct caacgcagga gagcgcgcca atttccagcc
     3661 acacggcggt gacgcgctta acatcgtgct gctccgcctg ccgttggata atttcaacgg
     3721 cgctctggca aagagacaac tcatgcattt tcgccactcc tgcgaccaaa cagcagggcg
     3781 cgacgccctg cgtgtggtac atccggattc gtgactggca gcgaaaggat cattcgtgcg
     3841 cagtcatccg tcaggcgttg gccctcttca atcgacatgc tgtgcgaaag cggcgacatc
     3901 agcgaacagg agagatattg cgaaacaccg tccagttcgc caacggtaaa ggtcatagtg
     3961 ccatacggca gttgcagacc aattttttca ctcactttgc gcagcggcca gagttgatcc
     4021 gggccgggga agataactgc actcagcatc cacggggtga tcacacaccc cgtccactga
     4081 ccttcgaaca gcgtaaaatc agaaacatac accggcattg aaggatgcag aaaagagaga
     4141 tcgtgcatcg aacgccgggc aatttcttca aacgctgcct gtacttgcgc cttcggggag
     4201 gtctggaaac ctgctatctc ttcagtcatg aatcgcctcc cgtgggatgg cttccacgcc
     4261 tgattcgcgc agcgcagcca gaacctgctc aagcgcaggt tcaatcattg cttcaaccgt
     4321 cggcgttaag ccgatgtgtg gctccagcga ttccgggatc acgccgacca gggtcagctt
     4381 tttcggaaac tcaccggtga agcgcagggc cgacaatacg tcggccaggc caagctgatg
     4441 cggagagatt ttgttggtaa acaacgccgg aacttcttca tcccgcagga tcatcatcgt
     4501 tcccggcgtg ttctttttcg acacaatggc atccgcaata atcagatgat cgcgatttgc
     4561 catgtcgcca agcagctcca ttcccgccgt gccgccatcg aggatctcaa cataatccgg
     4621 cagaatgtat cgttgctcta acgcttcgac aatccgcaca ccgatggctt catcggtcag
     4681 caaaatattg ccgaccccta agactaaaat acgcattaca gaaccttcac tgaaaccact
     4741 tcgttgccgt cggcatccac tacgtgtacc gcacaggcca tgcacgggtc gaaggagtga
     4801 atggtacgca ccacttccag cggtttattc ggatcagcaa ccggtgtacc caccagcgac
     4861 tgctcgtaag gaccgacgtc atcattgaag ttacgcggac cagagttcca ggttgatgga
     4921 acaactgcct ggtagttact gataataccg tctttaataa ccatccagtg agagagcata
     4981 ccgcgcggcg cttcgaggaa gccaacacct ttgaactcac ccgttgccgg aatgttcggt
     5041 ttcacaaagg tggtgtgatc gcctttgccg atattggtga tcagtgcact gtattgattt
     5101 tgcaggatat cctgcaattc gcagcagtga acggtacgac caataatacg gcccagcgtg
     5161 gagtgcagct gcgccacttc cagcgtgttg ccagtcagtt tctgataaat cgcaacgatt
     5221 tcattcagtt tgttctgggt agattcgcga cctgccgcca gtttcaccag catattagcc
     5281 agcggaccca cttctaccgt tttgccgtag aaagtcggtg atttcaccca ggaatatttc
     5341 ccgtcgtcag accaaccatc ataagccgga atggtggtac cttcccacgg tgcctgcggc
     5401 gcttcgtctt tataccagga gtgcttcgcg ctttcctgaa tgcctttgat caggtattca
     5461 tcggaatgag aagtgatcgg acgatacgag gacagatccg cattctcaat gtagccgccc
     5521 gggaacagga agctgccgtt tttactgtcg gtcgggaatt ccggcacgct caggtagttc
     5581 accgcacctt taccgcgcgt cagccattcc gggtagaacg cggcaataac tgcggtatca
     5641 accttataaa cctgttcaac aaagtcgctc agcttgtcga tgaaagactt gatgtacatc
     5701 aggcgctcaa ggttcagcac gcccaaaccg tcgaggttga tcgggttcgc gacaccacct
     5761 accgccaggt tctgaatgtg cggcgtttta ccgcccagca gcgccacgac gcggttagcg
     5821 tcacgctggc actccagcgc ctgcaggtag tgcgctaccg caatcaggtt cacttccggc
     5881 ggcagtttca tcgccgggtg acccccgtag ccattggcga aaatacccaa ctgaccgctg
     5941 gcaaccagat ctttgatctt gttctgaact ttagtgaact cttccggact gttcagatgc
     6001 caggtcgaaa cgcctttcag catttcggag gctttggttg ggtcagcttg cagtgcagaa
     6061 gtgatgtcca cccagtccag cgccgaaagc tgatagaaat gaacgatatg gtcatgcgtg
     6121 gtgtgcgcag ccagaatgat gttacggatg tattgcgcgt taaccggaac gtcgatattc
     6181 agcgcacttt ctgccgcacg aacggaagac agcgcgtgag tggtagtaca tacgccacag
     6241 atacgttgca caatcatcca tgcatcgcgc ggatcgcggt ttttcacgat ctcttccatg
     6301 ccgcgccaca tggtaccgga agcccatgct ttcgaaacga cgccattttc gatttcgcaa
     6361 tcgatgcgta aatgcccctc aatacgggtt accggatcaa tagtaattct ctggctcatg
     6421 ctttgctcgc ctcatgacga ttatgatcgt tttgttttaa aggaggaagt atcggcagta
     6481 gacgaatgag tacgatgtaa gcgcaaatct caatagccac aaaaccaata gaaatcaaca
     6541 gttcttccca ggtcgggaag taggcgtaac cgccgcccgg gttgaatgcc accagcgaat
     6601 aggtcagacg ccaggttgca caacctaaca gtgcgctcag tgctgacagg aacagcatgc
     6661 gggaatcatt acgcagcttc gccacacgca gaacgaccag cgggaagagc atcagcagga
     6721 cttcaatcca gaacatcacg gagtagaagt caccggcaaa cgctaacgac agcttgtcgc
     6781 gatagataag ctcgccaaag cgcagcacga tgaaaatcgc cagcaacaca ctgatggtgt
     6841 tggtcagctt gacgaacaga ctcttttcat ccggaccgtt gccacgcaga cccgcctgca
     6901 ccagcgaacc ttcaaagatg acaatcgaga agcccatgat gaacgccgtc agcagcgaga
     6961 acagcggcaa catttcatag ctctgccaca acggatgcac cttgtagccc gccgagatca
     7021 tcagtgaccc cattgaagac tggtgcatgg tcggcaacag cgcgccaagt gcaatgatga
     7081 agaacataac cttgttcaga cgcttgagag aaaccttcca gcccagacgt tcaaacagtg
     7141 ccggagcaaa ctccagtgcc atcacgccga tatagatggt catacagacc gccgtctcga
     7201 acagtaccga gttcacgttg aagtgacccg gaatgtagaa gtacggcagg ttccagtagc
     7261 gacccacgtc gatagtgatc gacaagccac ccagtgagta accaaacaga ctcgccaaca
     7321 gcgccggacg caccagcgga tggtattgcc cacggttaaa gacgtatacc gcccacgcca
     7381 gcgcccagcc gccacaggca aagccggtgc caatcagcag gtcaaacgcg atccacacgc
     7441 cccacgggaa gccgccgttc aggtcagaga cagagcccag accgaacacc agacgcttca
     7501 caatcaggag catacagatg acgattaacg gtccaaaaat catgaccggt ttactgatga
     7561 ttttgccgcc cagcggttgt ggatcatgac tcatgatcgt ctcctccgtc gtgatggtcg
     7621 tttttggtgt tgcgacgaac cagcacggtt aagcccgcca gcacagccag tggtagcatc
     7681 atgcctttat acagggtgtg ttgaatattt tcggaacgcg caccggtaga aagatcgtcc
     7741 agtttcggca ggtcgagatt ttcataaggc acacccgtca gtaccagaac ctgagtaccg
     7801 ccgccctctt tctcgccgta cagatgcgga taatatttcg gcaccgtatg caggtaagtg
     7861 tcgccagatt tcagcgtctg acgcggatag tggtattcgc tgccaggctt cagcgccaga
     7921 cgttttttcg cctccgccat cagctcttca cgcgtaccga aaatcaccgc gcccgccggg
     7981 cacacttcta cgcagccagg cagaccgcct ttatcgagac gttccacacc tttctggttg
     8041 cacagctcgc acttatgcag cgcaccaaac gggttgttgt agtcgtactt cggcacgttg
     8101 tacggacagg cgaccatgca gtaacggcaa ccggtgcaca cgtctttgtc gtaatggaca
     8161 atgccggttt tcggatcttt tttcagtgca gagaccgggc acacagagac acagttcgga
     8221 tcgacgcagt gcatacactg tttcttaatg tacgcgtagc cgttctcctc ctggtctttg
     8281 ttgacccctg tgccgctggt ccacacctga atgatgttat tggtatacgg cgacagtttg
     8341 tcgttgttcg accaggtctg ttccccttgc gggttacgtt cagggaagtt gatatcctga
     8401 cacttggtga cgcaagcctg gcagcctacg cacaaggtcg agtcgtacaa catccccagc
     8461 gatcccggaa ttggcgggcg gttttcagca gccgcatgac tgacagacgg cagcgcgccc
     8521 gtcagcaatg ccccgcagga ggctgcttta ataaaattac gtctgttcac ggttattctc
     8581 cccgtgagtc agcgttatct ttcttttgct gacgacccag ttcacgcacc gccatcacgc
     8641 tgacaccggc aaccagccca accacaccgc cgagcaaacc aatagcgcct gcagagacgt
     8701 tgccgccctc tttagcgtta acatccggtt tctgtgaacg cggagtctga ttttcgacgt
     8761 tggcaagctg atggatgcct ttatggaagc cgataccttc ttcgttacag ccatagcatg
     8821 ggtgaccaat cgctaccggc cacacgccgc caacatcgca gaattgcagc gttgagcagt
     8881 tgccgtaagt ttctggccct ttacagccga ggtggtacag gcaccagcct tcacggtggc
     8941 cttcatcacc gaactctttg gcaaaacgac cagcatcgaa gtgcgggcga cgttcgcagt
     9001 gttcgtgaat cagacggcca taggcgaagg tcggacggtt tttgtcatcc agtttcggcg
     9061 gtttgccgta agtgatgatg tgcgcaacgg tcgcgaggaa gttgtgcggg ttcggcgggc
     9121 agcccggaat attgataacg gttttgcctg gcagaacttc ttgcaggctg actgcgccag
     9181 ttgggttaac tccagctgcg gcaacaccgc cccacgcaga gcaggaaccg atagcgataa
     9241 tcgctgctgc gccttctgcc gctttgcgga tgtgatccac aatcggctca ccagcaacca
     9301 tgcaataaat accgttatct tttaatggga tggaaccatc cacaaccaac acatactgcc
     9361 ctttgtactt ctcaagtgcg ttatgtttgt tctcttcgac ctgatgaccg aaggcggcgg
     9421 aaagcacttc gtgatactcc agagagatag tctccagcac gaggttttct accgttggat
     9481 gcgttgcacg aagcagagat tccgtacaac cggtgcactc ctgcgcgcca atccagataa
     9541 ctggcggacg ctgcgggtta gtaaccgatt cggccatctc tgcagcggct ttgctactta
     9601 accccatggt ggcggctaat gctgcacaaa gcttcatgaa atcacgacgg ttaatgccgt
     9661 gagaatggat gagggtgtta tctccagtca tttatagtta ttccgttgcg aagacctggc
     9721 atatattttg cctcaatcgc aaaatcaata atgcgatcga tgcgccattt accacacatt
     9781 tattatggtt atcgatatca tgatactgcg gcggggcgct aaaacgaagg gaagaagcat
     9841 tagtgtaatt aattaaagca agataatacg tatgtttgat caattttcga ggcgataacc
     9901 ggccataaaa aagcgcaacg tagaacagga attattctca cgaaagaaat ccattaccat
     9961 ctctttatcc agtccatagc ggcgcgtcag aatacccgct tcccaggcgc taagctggcg
    10021 atctccggtc ttttcgtaca tccggtgtga gtagcctaat acaaaaccgc gtttataatc
    10081 ggcacagaat tttgtcacat taaccgcact gtcggcatgc gttgctttta atcccgccat
    10141 taagccttta ccaaaatggt tattcatcgt tcacctcatt cgctatatat cgccatatat
    10201 agcgatattt taggcaaaga acgacttttg cgcacgctat tttttcatgc ttgtttaaga
    10261 cgtaatccag taagagataa acctatcagg agagatattt atcgaaccat ttcagtgtcc
    10321 tttgccaggc aagatcggcg gcagatttgt cataacgggg cgtggaatca ttatggaatc
    10381 cgtgattaac ccccggatag atatacgcct cataaacctt attattggct ttcaacgccg
    10441 cctcgtaagc aggccagccc tcgttgattc gggtgtccag ttccgcgaag tggagtagta
    10501 aaggcgcttc aatcttcgcc acatcggcag tgggtgcctg acgaccataa aacggcaccg
    10561 cgcaggccag ttccggatac gcgacagccg ccgcgttcga tacgccaccg ccatagcaaa
    10621 atccggtaat acccactttg cctgccgctt gtggatagcg ttgcataaac tcaattgcgg
    10681 caaagaaatc attcatcagt ttggttggat caacctgctg ttgcagctca cgacctttat
    10741 catcatttcc cggataacct ccaacggaac ttaagccgtc aggtgccagg gcgatatacc
    10801 ccgccttcgc cactcgccgt gccacatctt cgatatacgg attcagtcca cgattctcat
    10861 gcaccaccac cacggctggc gttttgccgc tcatttttgc gggtttcacc agataacccc
    10921 gtacctcgcc gtgaccattt ggcgaaggat acatgatgta ctcagcaaca atctccgggt
    10981 cggtaaactc tacctgagtc gccagcgcat aatttggctt gagcaaatca aacaacgcta
    11041 atgccgtcat cccgcccacc gcatacttcg ccgcaaggtt gaggaactca cgtttcgaaa
    11101 ttttcccgtg agcgtaatag tcgtagtaat ccaacaactc ttgtgggaaa tctttggcgg
    11161 ttaaacgcgg catcgttgca ctcctcagtt ggtgtttttt taagcaaagc ataagcacgt
    11221 gtttttgccc agtttttcgt cactctgtga gccagactac gggatacgcg ctggcgaatc
    11281 gctaaactag aaacattgtt tcgaaattga acggtggaaa ggagaggtca tggtctggtt
    11341 agcgaatccc gaacgttacg ggcagatgca ataccgctat tgcggaaaaa gcggtttacg
    11401 cctgcccgcg ttatcgctcg gtttatggca caatttcggt cacgttaacg cgctggaatc
    11461 acagcgtgcg atcctgcgta aagcgtttga tttgggcatt acgcactttg atttagccaa
    11521 caattacggg ccgcctccag gaagcgcaga agagaacttt ggtcgcctgc tgcgggagga
    11581 ttttgccgct tatcgcgatg aactgattat ctctaccaag gctggctacg atatgtggcc
    11641 cggcccttac ggctctggcg gttcacgtaa atacctgctc gccagcctcg accaaagcct
    11701 gaagcgtatg gggcttgagt atgtcgatat cttttactct catcgcgtcg atgaaaatac
    11761 gccgatggaa gaaaccgcct ctgcgctggc tcatgcggta caaagcggta aggcgctgta
    11821 tgtcgggatc tcctcttact cgccagagcg gacgcaaaaa atggtcgagt tgctgcacga
    11881 gtggaaaatt ccgcttttaa ttcatcaacc ttcgtacaat ttactgaacc gctgggtgga
    11941 taaaagcggc ctgctggata ccctgcaaaa taacggcgtg ggctgtattg cctttactcc
    12001 tctggctcag ggattgctga ccggaaaata tctcaacggc attccgcaag attcacggat
    12061 gcatcgtgaa gggaataaag ttcgtggtct gacgccgaaa atgcttaccg aagccaacct
    12121 caacagcctg cgcttattga atgaaatggc acagcagcgt ggacaatcaa tggcgcaaat
    12181 ggcgttaagc tggttgctga aagatgatcg cgtgacgtcg gtattgattg gtgccagccg
    12241 cgcggagcaa ctagaggaga acgtgcaggc gctgaataat ctgacattta gcaccgagga
    12301 gctggcgcag attgatcagc atatcgccga tggcgagctg aatctgtggc aggcgtcgtc
    12361 cgataaatga cctgttaata acgggcgcgc gaaccgcgcc cgaatcagat cagtgattat
    12421 gacgagtcag tcggtcaaga tagcccatca caaatgcaga aagcacaaac gtcagatgga
    12481 taatgacgta ccacatcagt ttgttatccg ggacattttt cgcatccata aagacgcgca
    12541 gtaagtgaat ggaagaaatt gccacaatcg acgctgctac tttgtttttc agcgacgttg
    12601 cgtccatttt ccccagccag ttcagcttct ctttgttctc ggagatatcc agctgtgaga
    12661 cgaaattctc ataaccggaa aacatcacca tcaccagcaa accgccaacc agcgtcatat
    12721 ccaccagcga cagcaacacg aggatcaaat ctgattccgc catcgagaag atattcggca
    12781 gtacgtgaat aatctcctgg aagaacttca gcgccagggc aactaacgcc agcgaaaggc
    12841 caaagtacac gggggcaagc agccagcgag aagcatacat tgcattttca agaaaacgtt
    12901 ccatacattc ctgtcgtgta gttaaaacgg gcaacagtat atcgcaattg agtaaacagc
    12961 acgcaacagc aaatcaacat atacgttaaa cctttgtcgc ataattctga ggtttaggga
    13021 tcttcctggt tcccttatca acgcatcgag agcgtctacc cttaaaggac gttgaacctg
    13081 aagggagaaa aacgatgtct catttaaaag acccgaccac gcagtattac actggtgaat
    13141 atcccaaaca gaaacaaccg acgccaggca tccaggcgaa gatgacaccg gtaccggatt
    13201 gcggcgagaa aacctatgtt ggtagcggtc gcctgaaaga tcgtaaagca ctggtgacag
    13261 ggggcgattc cggaataggt cgcgctgccg ccatcgctta cgcgcgtgaa ggggctgacg
    13321 tggcgatcag ttatcttccc gtggaagaag aagacgctca ggatgtgaaa aagatcattg
    13381 aagaatgcgg acgcaaagcc gttctgctgc caggcgattt aagcgatgag aaattcgccc
    13441 gttcgctggt tcacgaagcg cacaaggcgt taggcgggct ggatattatg gcgctggtcg
    13501 ccgggaaaca ggttgccatt ccggatattg cagacctcac cagcgaacag tttcaaaaga
    13561 cctttgccat taacgttttc gcgctgttct ggctaaccca ggaagcgatc cccctgctac
    13621 cgaaaggtgc aagtatcatc accacttcgt caatccaggc ataccagcca agtccgcatt
    13681 tactggacta tgcggctacg aaggcggcga ttctgaacta cagccgtggc ctggcaaaac
    13741 aggtcgcgga gaaaggtatt cgggtgaata ttgtcgcgcc aggccctatc tggacggcac
    13801 tgcaaatttc cggcggacaa acgcaggata agatcccgca gtttggtcag aaaacgccga
    13861 tgaagcgtgc ggggcaaccg gcggaactgg cccctgtata tgtttatctg gcaagtcagg
    13921 agtcgagcta cgtcaccgca gaagtgcacg gcgtgtgcgg cggcgagcat ttaggttaaa
    13981 aaaatgcccg gttgtgaaaa gcaaccgggc atcattgtga attcactctt acctgatgtg
    14041 gcgaaaacgc cttatccggc ctacaaacgc acgaagattc aacatgttgc aatagatttt
    14101 gtaggcctga tgagcgtagc gcatcaggca attctacttt acttcgcttt ggcggtttct
    14161 tcgccgacca gacctatctt caggtaaccc gcctgatgca gcgtatccat taccttcatc
    14221 aacgtctcgt aatcgacggt tttgtccgct cggaagaaga tggtggtgtc tttcttgcct
    14281 tcggttaacg cattcaacgc cgtaatcatt gtttcatcgg tgaccggatc gttaccgata
    14341 aacatcgagt tgtctgcctt caccgacaga taaaccggtt tttccgggcg cggctgcggc
    14401 gtgctggtag aagcaggcaa gttcaccttc acatctaccg tcgctaacgg tgccgccacc
    14461 ataaagataa tcagcagaac caacatcacg tcgataaacg gcgtcacgtt gatatcatgc
    14521 atttcgccgt tatcgtcgag gttttcgtta agatgcattg ccatcggaca ttatcctgcg
    14581 cgtaattttt gtgcgacacg aaccggatgc gcagcggcgc tggcttccag atccaggtca
    14641 cggctttgca gcaacaatac ctgcgctgca acatcaccca gcatcgcttt aaagccgcca
    14701 atctggcgtg caaatacgtt atagataacg accgcaggaa tcgctgccac gaggccgatt
    14761 gccgttgcta acagagcttc tgcgataccc ggcgcaacga ctgccaggtt agtggtctgc
    14821 gtttgcgcga taccaataaa gctgttcatg atgccccaga ccgtaccaaa cagaccaacg
    14881 aacggagaaa tcgcgccgat ggttgccaga tagccgttac cgcggcccat ttgacgaccc
    14941 actgcggcga cccgacgctc cagacggaag ctggtacgtt ctttaatacc ttcgttatcg
    15001 tcgctgcctt ctgacagttc cagctcattc tgcgcttcat tgagcaaatg caggcttaag
    15061 cttttgctac caaaatcagc ggcgatatcg ttggcctggt ttaaggaacg cgcttcagcc
    15121 agcagttgct gctcgcgctt aaggcgacgc ttctgattga agaactctac gctcttactg
    15181 aagaagattg cccaagtgac tacggaggcc aaaataagcc caatcatcac gcacttaacg
    15241 acaatatcgg cgtgctgata cataccccag acggaaaggt ccgtctgcat caaattatta
    15301 cccactgtgt atctccagga cgcaagtcac aaaatctgcg cataataata tcaaaatgac
    15361 gtcgaattga tagtcgttct cattactatt tgcatactgc cgtacctttg ctttcttttc
    15421 cttgcgttta cgcagtaaaa aagtcaccag cacgccattt gcgaaaattt tctgctttat
    15481 gccaattctt caggatgcgc ccgcgaatat tcatgctagt ttagacatcc agacgtataa
    15541 aaacaggaat cccgagatgg cggacaaaaa gcttgatact caactggtga atgcaggacg
    15601 cagcaaaaaa tacactctcg gcgcggtaaa tagcgtgatt cagcgcgctt cttcgctggt
    15661 ctttgacagt gtggaagcca aaaagcacgc gacgcgcaat cgcgccaatg gcgagttgtt
    15721 ctatggacgg cgcggaacgt taacccattt ctccttacaa caagcgatgt gtgaactgga
    15781 aggtggcgca ggctgcgtgc tatttccctg cggggcggca gcggttgcta attccattct
    15841 tgcttttgtc gaacagggcg atcatgtgtt gatgaccaac accgcctatg aaccgagtca
    15901 ggatttctgt agcaaaatcc tcagcaaact gggcgtaacg acatcgtggt ttgatccgct
    15961 gattggtgcc gatatcgtta agcatctgca gccaaacact aaaatcgtgt ttctggaatc
    16021 gccaggctcc atcaccatgg aagtccacga cgttccggcg attgttgccg ccgtacgcag
    16081 tgtggtgccg gatgccatca ttatgatcga caacacctgg gcagccggtg tgctgtttaa
    16141 ggcgctggat tttggcatcg atgtttctat tcaagccgcc accaaatatc tggttgggca
    16201 ttcagatgcg atgattggca ctgccgtgtg caatgcccgt tgctgggagc agctacggga
    16261 aaacgcctat ctgatgggcc agatggtcga tgccgatacc gcctatataa ccagccgtgg
    16321 cctgcgcaca ttaggtgtgc gtttgcgtca acatcatgaa agcagtctga aagtggctga
    16381 atggctggca gaacatccgc aagtagcgcg agttaaccac cctgctctgc ctggcagtaa
    16441 aggccacgaa ttctggaaac gagactttac aggcagcagc gggctatttt cctttgtgct
    16501 taagaaaaaa ctcagtaatg aagagctggc gaactatctg gataacttca gtttattcag
    16561 catggcctac tcgtggggcg ggtatgaatc gttgatcctg gcgaatcaac cagaacatat
    16621 cgccgccatt cgcccacaag gcgagatcga ttttagcggg accttgattc gcctgcatat
    16681 tggtctggaa gatgtcgacg atctgattgc cgatctggac gccggttttg cgcgaattgt
    16741 ataacattgc cacttttgga caattttgca gacatttaat tgtgaaaagt cttaaattgt
    16801 tgcgtccggg atcaaggcgt cccggacgaa tcaggagtac aataggcaga taaaggctta
    16861 aacgctgttc cacaggaaag tccatggctg ttattcaaga tatcatcgct gcgctctggc
    16921 aacacgactt tgccgcgctg gcggatcctc atattgttag cgttgtttac tttgtcatgt
    16981 ttgccacgct gtttttagaa aacggcctgc tgcccgcctc atttttgcca ggcgacagct
    17041 tgttgatact ggcaggcgca ttgattgccc aaggggttat ggattttctg cctacgattg
    17101 cgattctgac cgccgcagca agtctgggct gctggctaag ttatattcag gggcgctggt
    17161 tagggaatac caaaacggtg aaaggctggc tggcacagct tcctgctaaa tatcaccagc
    17221 gcgccacctg catgtttgac cgccacggtc tgctggcgct gctggctgga cgttttcttg
    17281 catttgtccg tacgctgctg ccaaccatgg cgggaatttc cggtctgcca aaccgccgct
    17341 tccagttttt caactggtta agtggattgc tgtgggtcag cgtggtaacc agttttggct
    17401 atgccttaag tatgattccg ttcgttaaac gccatgaaga tcaggtaatg acgttcctga
    17461 tgatcctgcc aattgccttg ttaaccgctg gcttgttagg cacgctgttt gtagtgatta
    17521 aaaaaaaata ctgtaacgcc tgacgatttt ccccgttccc ggctgctgta ccgggaacgt
    17581 atttaattcc cctgcatcgc ccgcattctt gccgcatctt cccccggcgt cacaccgaag
    17641 taacgtttaa actcacggct aaattgcaat gcgctttcat agtcgacgcg catcgctgct
    17701 gcgctggcct tcatgccgtc atggatgatc atcatccgcg ccttatgcag acggtaattc
    17761 ttcaaatact gcaacggcga ggtgctggtg acagacttaa aattatgatg gaacgccgat
    17821 acgctcatgt tggcttctgc cgccagttgc tcgacgctca ggttttcggt gtatttattc
    17881 tcaatccgtt tcagcacgcg gctaatcaga ctgaagtgag tctggcgact gaccagcgcc
    17941 agtaacgcgc cgccgcaagg tccggtcagc acgtagtaca gaatttcgcg gatgatctgt
    18001 ttgccgagaa tacgcgcatc cagtggtcgc tccatcacgt cgagtaaccg ctccgccgcg
    18061 cataaaatct cttctgataa cgtggcggag ttaatcccgc tggctgccat cgacggctgg
    18121 aaatgctcat cttcgccaat gtccatcaac agttcctgta actgcaaaat atcgacattg
    18181 agacgcaatc ctgccagcgg cacctctgac gtggcatagg tttcgcactc aaacggcaac
    18241 ggcaccgtca gcagcaggta ttcattggca tcataacgaa acacgcgttc attgatataa
    18301 ccgattttat gcccggaaaa gagaattatg atgccaggct cgtacatcac cggtgtacgt
    18361 gcgaaaggcg tctcgccata caacaaacgc acatcgggca acagttctga caaactattt
    18421 tctttatttt tcagtttatt aactttatcc gccagcaagc ggcaaatctc ttcacgtttc
    18481 atatcgcgta atttcttagg aataatgcgg caatttgatt gtgcgcaatt ttgtagcatt
    18541 tctccagcac tctggagaaa taggcaagac attggcagaa atgagcattg agagccaggg
    18601 cgctggcgat cacaatgaaa aacatcaggc agatcgttct ctgccctcat attggcccag
    18661 caaagggagc aagtaatgaa caactttaat ctgcacaccc caacccgcat tctgtttggt
    18721 aaaggcgcaa tcgctggttt acgcgaacaa attcctcacg atgctcgcgt attgattacc
    18781 tacggcggcg gcagcgtgaa aaaaatcggc gttctcgatc aagttctgga tgccctgaaa
    18841 ggcatggacg tgctggaatt tggcggtatt gagccaaacc cggcttatga aacgctgatg
    18901 aacgccgtga aactggttcg cgaacagaaa gtgactttcc tgctggcggt tggcggcggt
    18961 tctgtactgg acggcaccaa atttatcgcc gcagcggcta actatccgga aaatatcgat
    19021 ccgtggcaca ttctgcaaac gggcggtaaa gagattaaaa gcgccatccc gatgggctgt
    19081 gtgctgacgc tgccagcaac cggttcagaa tccaacgcag gcgcggtgat ctcccgtaaa
    19141 accacaggcg acaagcaggc gttccattct gcccatgttc agccggtatt tgccgtgctc
    19201 gatccggttt atacctacac cctgccgccg cgtcaggtgg ctaacggcgt agtggacgcc
    19261 tttgtacaca ccgtggaaca gtatgttacc aaaccggttg atgccaaaat tcaggaccgt
    19321 ttcgcagaag gcattttgct gacgctaatc gaagatggtc cgaaagccct gaaagagcca
    19381 gaaaactacg atgtgcgcgc caacgtcatg tgggcggcga ctcaggcgct gaacggtttg
    19441 attggcgctg gcgtaccgca ggactgggca acgcatatgc tgggccacga actgactgcg
    19501 atgcacggtc tggatcacgc gcaaacactg gctatcgtcc tgcctgcact gtggaatgaa
    19561 aaacgcgaga ccaagcgcgc taagctgctg caatatgctg aacgcgtctg gaacatcact
    19621 gaaggttccg acgatgagcg tattgacgcc gcgattgccg caacccgcaa tttctttgag
    19681 caattaggcg tgccgaccca cctctccgac tacggtctgg acggcagctc catcccggct
    19741 ttgctgaaaa aactggaaga gcacggcatg acccaactgg gcgaaaatca tgacattacg
    19801 ttggatgtca gccgccgtat atacgaagcc gcccgctaag ctttttacgc ctcaaacttt
    19861 cgttttcggg catttcgtcc agacttaagt tcacaacacc tcaccggagc ctgctccggt
    19921 gagttcatat aaaggaggaa cgtatggcta atccaaccgt tattaagcta caggatggca
    19981 atgtcatgcc ccagctggga ctgggcgtct ggcaagcaag taatgaggaa gtaatcaccg
    20041 ccattcaaaa agcgttagaa gtgggttatc gctcgattga taccgccgcg gcctacaaga
    20101 acgaagaagg tgtcggcaaa gccctgaaaa atgcctcagt caacagagaa gaactgttca
    20161 tcaccactaa gctgtggaac gacgaccaca agcgcccccg cgaagccctg ctcgacagcc
    20221 tgaaaaaact ccagcttgat tatatcgacc tctacttaat gcactggccc gttcccgcta
    20281 tcgaccatta tgtcgaagca tggaaaggca tgatcgaatt gcaaaaagag ggattaatca
    20341 aaagcatcgg cgtgtgcaac ttccagatcc atcacctgca acgcctgatt gatgaaactg
    20401 gcgtgacgcc tgtgataaac cagatcgaac ttcatccgct gatgcaacaa cgccagctac
    20461 acgcctggaa cgcgacacac aaaatccaga ccgaatcctg gagcccatta gcgcaaggag
    20521 ggaaaggcgt tttcgatcag aaagtcattc gcgatctggc agataaatac ggcaaagccc
    20581 cggcgcagat tgttatccgc tggcatctgg atagcggcct ggtggtgatc ccgaaatcgg
    20641 tcacaccttc acgtattgcc gaaaactttg atgtctggga tttccgtctc gacaaagacg
    20701 aactcggcga aattgcaaaa ctcgatcagg gcaagcgtct cggtcccgat cctgaccagt
    20761 tcggcggcta acatgcaaat tctcccggtg acggtaatgt tccgctaccg gacttttcag
    20821 aaatcattta ttcccctcgc gtcccgcccg ttgttactct tccttgttca ggaatgccaa
    20881 atataaggac atcatcatgc a
//
LOCUS       DAZPDU010000065        20827 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZPDU010000065 DAZPDU010000000
VERSION     DAZPDU010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20827
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.066"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(131..1780)
                     /gene="actP"
                     /locus_tag="ACHURS_002173"
     CDS             complement(131..1780)
                     /gene="actP"
                     /locus_tag="ACHURS_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFR1237898.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKLVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSITVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1777..2090)
                     /locus_tag="ACHURS_002174"
                     /pseudo
     CDS             complement(1777..2090)
                     /locus_tag="ACHURS_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
     gene            complement(2290..4248)
                     /gene="acs"
                     /locus_tag="ACHURS_002175"
     CDS             complement(2290..4248)
                     /gene="acs"
                     /locus_tag="ACHURS_002175"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFR1237899.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEKMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLTCGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4642..6078
                     /gene="nrfA"
                     /locus_tag="ACHURS_002176"
     CDS             4642..6078
                     /gene="nrfA"
                     /locus_tag="ACHURS_002176"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFR1237900.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6123..6689
                     /gene="nrfB"
                     /locus_tag="ACHURS_002177"
     CDS             6123..6689
                     /gene="nrfB"
                     /locus_tag="ACHURS_002177"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFR1237901.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6686..7357
                     /gene="nrfC"
                     /locus_tag="ACHURS_002178"
     CDS             6686..7357
                     /gene="nrfC"
                     /locus_tag="ACHURS_002178"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFR1237902.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7354..8310
                     /gene="nrfD"
                     /locus_tag="ACHURS_002179"
     CDS             7354..8310
                     /gene="nrfD"
                     /locus_tag="ACHURS_002179"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFR1237903.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8330..10048
                     /locus_tag="ACHURS_002180"
     CDS             8330..10048
                     /locus_tag="ACHURS_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFR1237904.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWEFKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFCH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRAQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            10041..10424
                     /gene="nrfF"
                     /locus_tag="ACHURS_002181"
     CDS             10041..10424
                     /gene="nrfF"
                     /locus_tag="ACHURS_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFR1237905.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10421..11017
                     /gene="nrfG"
                     /locus_tag="ACHURS_002182"
     CDS             10421..11017
                     /gene="nrfG"
                     /locus_tag="ACHURS_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFR1237906.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTCAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11359..12672
                     /gene="gltP"
                     /locus_tag="ACHURS_002183"
     CDS             11359..12672
                     /gene="gltP"
                     /locus_tag="ACHURS_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFR1237907.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHRELLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12751..13440)
                     /gene="yjcO"
                     /locus_tag="ACHURS_002184"
     CDS             complement(12751..13440)
                     /gene="yjcO"
                     /locus_tag="ACHURS_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFR1237908.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13534..15681)
                     /gene="fdhF"
                     /locus_tag="ACHURS_002185"
     CDS             complement(13534..15681)
                     /gene="fdhF"
                     /locus_tag="ACHURS_002185"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(15262..15264),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HFR1237909.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMDHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15879..17345)
                     /gene="mdtP"
                     /locus_tag="ACHURS_002186"
                     /pseudo
     CDS             complement(15879..17345)
                     /gene="mdtP"
                     /locus_tag="ACHURS_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
     gene            complement(17342..19393)
                     /gene="mdtO"
                     /locus_tag="ACHURS_002187"
     CDS             complement(17342..19393)
                     /gene="mdtO"
                     /locus_tag="ACHURS_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFR1237910.1"
                     /translation="MSALNSLPLPMVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGRMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPA
                     AKPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVSNPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19393..20424)
                     /gene="mdtN"
                     /locus_tag="ACHURS_002188"
     CDS             complement(19393..20424)
                     /gene="mdtN"
                     /locus_tag="ACHURS_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFR1237911.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSINATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20443..20718)
                     /locus_tag="ACHURS_002189"
     CDS             complement(20443..20718)
                     /locus_tag="ACHURS_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFR1237912.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         4691 a         5518 c         5699 g         4919 t
ORIGIN      
        1 tatcattttc tacgactgtc aaaaatcgct cattttttaa tgagtttatt tgtttaatat
       61 tttgggaaaa ggtgatgcat gctggagagg aagagtattc cccggtcagg cgaccgggga
      121 aggggagaga ttaatgtgcg cggccttgct caacgccaaa gccggtctgg gagcggataa
      181 actgcgcgcg gaacagttca cgctcgcgcg ccccttccgc tgagttatcg gttgccgaga
      241 agaaccagat gccgaggaat gccacggtga tagagaacag cgccgggtat tcatacggga
      301 agatggcttt ttcgtgacca aggatctgta cccaaatcgt cgggccgagg atcatcagta
      361 ctactgcggt aatcagcccc agccagccac ccatcatcgc gccacgcgtg gtcagtttcg
      421 accagtacat agaaagcaga atgatcggga agttacagct cgccgcgatg gcaaacgcca
      481 gccccaccat aaaggcgatg ttctgattct caaacagcac gccgaggata atcgcaatca
      541 cgccgaggat cagtacggtg atttttgata cccgcagctc ttcacgttcg gtcgcgcctt
      601 ttttgaagac gttagcgtac aagtcatgcg aaaccgcgga tgcgcccgcc agcgtcagac
      661 ccgcaaccac cgcgaggata gtggcgaaag caaccgctga aataaaaccg aggaacaggt
      721 tgccgcccac tgcattcgcc aggtgaacgg ccgccatgtt gttaccacca atcagatggc
      781 ccgccgcgtc tttatattcc ggattcgcac caaccagcat gatcgcgccg aagccgataa
      841 taaaggtcag aatatagaag tagcccataa acccggtggc gtagaacacg ctcttacgtg
      901 cttcgcgggc atcgctgact gtaaagaagc gcatcagaat gtgcggcaag cccgccgtac
      961 caaacatcag tcccagaccc agagagagtg cggagatcgg atctttcacc agcccgcccg
     1021 gcttcatgat gtcgacacct ttcgggtgta ccgccatcgc ttcactgaac agattgttga
     1081 agctaaagcc gacgtgtttc atcaccataa aggccataaa gctggcaccg aacagcaaca
     1141 gcacggcttt gataatctgt acccaagtgg tcgccagcat gccgccgaac aggacgtaca
     1201 tcatcatcag cacgccgacc agcaccaccg caatgtgata gttaaggcca aacagcagct
     1261 cgatcagttt acctgcgccc accatctggg cgataaggta aagcgccacc accaccagag
     1321 aaccacaggc cgaaagaata cgaatcggcc cttgtttcag acggtaagag gccacatcgg
     1381 caaaggtgta gcgccccagg ttacgcagac gttcggcaat caggaacaaa atgatcggcc
     1441 agcccaccag gaagcccagc gagtaaatta agccgtcata gccggaggta aacaccagcg
     1501 cggagatccc caagaatgag gcggcggaca tatagtcccc ggcaatcgcc agcccgttct
     1561 ggaagccagt gatattgccg cctgcggtgt agtagtcgct acgagaacgt acgagttttg
     1621 atgcccagta ggtaatgccg agcgtaaaca cgacgaaaat caggaacata ataatcgcct
     1681 gccagttcgt tggctggcgc tctacggccc cgctaatagc atccgcggcg ttagctgcga
     1741 aagggagtgt ggcggcaagc gccgtcagaa ctcttttcat gatgcttgta cctcatgcag
     1801 gacttcatta ttaagacggt cgaattcgcc gttcgcccgc cagatgtaga taccggtgag
     1861 aacaaaggag atcacaatca ctccaacacc aattggaata ccgcgtgtga cgctggtgtt
     1921 cggattcagc ggcgtgccag ccagccgggc gcgaaggcga tcagtaaaat aaagccgata
     1981 taaactgcca gcataataat cgacaggatg gtggcaaacc gttgccgttt ttcgactaac
     2041 tccctgaaat gcgcattgtc ttctatccgc tgataaatag tgccattcat cacagattct
     2101 ccagaggtaa tgtagggatt gttttaattc ccgctccctt atgggagaag gttaacgctc
     2161 gggtaaccct tgccgaatgt aggccggata aggcgtttac gccgcatccg gcaatcaatg
     2221 cctgatgcga cgctgtcgcg tcttatcagg cctacaaacc gttaccgact cgcatccggc
     2281 aattgtgggt tacgatggca tcgcgatagc ctgcttctct tcaagcagct tctcgactac
     2341 gccaggatcg gcaagcgtcg aggtatcgcc caggttgctg gtatcgcccg ccgcaatttt
     2401 gcgcagaata cggcgcataa ttttgccgga gcgggtttta ggcagggagt cggtccagtg
     2461 cagcacgtct ggcgtcgcca gcgggccaat ctctttacgc acccagttgc ggacttctgc
     2521 gtacagttct ggtgacggtt cctccccgtg attaagcgtg acgtaggcgt agatcgcctg
     2581 acctttaata ttgtgcggaa tacctactac ggcggcttcg gcaatcttcg gatgcgccac
     2641 cagcgccgac tcaatctctg ccgtccccag acggtgaccg gagacgttca gcacgtcgtc
     2701 cacacgcccg gtgatccagt aatagccatc ttcatcgcga cgcgcgccgt cgccgctgaa
     2761 atacatattt ttgaaggtgg agaagtaggt ctgttcaaaa cgttcgtgat cgccaaacag
     2821 cgtacgcgcc tgacccggcc aggagtcggt gatcaccagg ctgccttcgg tagcgccttc
     2881 cagcgggtta ccttcgttat cgaccagcgc cggttgcacg ccgaagaacg gacgtgttgc
     2941 cgaaccggct ttcagctcgg tagcgccagg cagcggcgtg atcatgaaac cgccagtttc
     3001 ggtctgccac caggtatcga ccaccggaca tttctcgttg ccgatttttt tccagtacca
     3061 ctcccacgct tccgggttaa ttggctcgcc cacggaaccg agaatgcgca gcgacgaacg
     3121 gtcggtaccc tcgatcgctt tatcgccttc cgccatcagc gcgcggatcg ccgtgggtgc
     3181 ggtatagaga atattgacct gatgcttgtc caccacctgc gccatacggg caggcgtcgg
     3241 ccagttgggt acgccttcaa acatcagcgt ggtcgcaccg caggtcagcg ggccgtacag
     3301 caagtaactg tgtccggtca cccagcccac atcggcggtg caccagtaga tatcacccgg
     3361 atgataatca aagacatatt taaaggtcag cgccgcgtac accagataac cgccggtagt
     3421 atgcagcaca ccttttggct taccggtaga accggaggtg tagagaataa acagcggatc
     3481 ttcggcgttc atcttttccg cctggtgctg atcgctcgct tgctcaacca ggtcgtgcca
     3541 ccacaggtcg cgcccttcct gccagtcaat tttcccgcca gtacgcttca gtaccaccac
     3601 atgctctacg ctggtgacgt tcgggttttt cagcgcgtca tcaacgtttt tcttcagcgg
     3661 aatactgcgc ccggcacgca caccttcgtc ggaagtgatc accagtcgtg agttggaatc
     3721 aataatgcgc ccggcaacgg cttccggcga gaagccgccg aaaatcaccg aatgcaccgc
     3781 gccaatgcgg gcgcaggcca gcatcgcaac cgcggcttcc ggcaccatcg gcatataaat
     3841 cgccaccaca tcaccttttt taatgcccag ctcgagcagg gtattggcga agcggcagac
     3901 gtcgcggtgc agctctttat agctgatatg tttgctctgg ctggcgtcgt cgccttccca
     3961 gatgatggcg gtacgatcgc cgttttcttg cagatggcgg tcaaggcagt ttgccgccag
     4021 attcagcgtg ccgtcctcgt accatttaat ggacacatta ccgggggcaa aggaggtgtt
     4081 tttcaccttc tggtaaggtt tgatccagtc aagaattttt ccctgttcgc cccagaaggt
     4141 atcaggtacg ttaatagatt gttgatacat cgcctcgtac tgctgagggt ttatcaggca
     4201 acggtctgcg atgttggcag gaatggtgtg tttgtgaatt tggctcatgc ttttgttctc
     4261 cttgtagggt gttaataata tgtggcataa gcgttaaatg taggggtatt ggcagttttg
     4321 tttagtattt gggcgacaga tcacgcaaaa gtagaattgt gcaaataaac ggcagggtaa
     4381 tttttgaagg tcagcaacaa aagttgatta attctttgag gaacatgcag ttatgcattg
     4441 ctgttgaaaa agaggaagat actaactaac tctaaagtgg tattttacat gcacttacaa
     4501 ttgattaaag acaacattca cagtgtggtt atttgttaca cataggggcg agcaatgtca
     4561 tgacagtgta ggtgcggtta ctgtcgtgaa aaacaataaa aaccgccatt gtaacaatgg
     4621 cgcaattcgg atgaagcccc tatgacaagg ataaaaataa acgcacgccg tatcttcagc
     4681 ttattgattc cttttttctt tttcacttct gttcacgctg aacaaacggc agctcccgca
     4741 aaacctgtaa ctgtggaagc gaagaatgaa acctttgccc cgcagcatcc cgatcaatat
     4801 ctctcctgga aagccacctc ggaacagtca gagcgtgttg acgccctggc ggaagatcca
     4861 cggctggtga tcctgtgggc ggggtatccc ttctcgcgcg attacaacaa gccgcgtgga
     4921 catgcctttg ctgtgaccga tgtgcgtgaa accctgcgta ccggtgcgcc gaaaaacgct
     4981 gaagatggtc cgctaccgat ggcgtgctgg agttgtaaaa gcccggatgt ggcgcgtctg
     5041 atccagaaag acggcgaaga tggctacttc cacggtaagt gggcgcgcgg cggcccggaa
     5101 atcgtcaaca acttaggttg tgccgactgc cataacaccg cctcaccaga gttcgccaaa
     5161 ggcaaaccgg agttaaccct ttcccgtccg tatgcggctc gcgcgatgga agccattggt
     5221 aaaccttttg agaaagccgg acgtttcgac cagcaatcga tggtttgcgg tcagtgccat
     5281 gtggagtatt acttcgacgg caaaaacaaa gcggttaaat tcccgtggga tgacggcatg
     5341 aaagtcgaaa atatggagca gtattacgac aaaattgcct tctctgactg gactaactcc
     5401 ctgtcgaaaa cgccaatgct gaaagcgcag cacccggaat atgaaacctg gacagcgggc
     5461 attcacggta aaaacaacgt gacctgtatc gactgccata tgccaaaagt gcagaacgcc
     5521 gaaggcaaac tctataccga ccataaaatt ggtaatccgt ttgataactt cgcccagact
     5581 tgtgcgaact gccataccca ggacaaagct gccttgcaaa aagtggtcgc ggaacgtaag
     5641 cagtcgatta acgacctgaa aatcaaggtt gaagatcaac tggttcacgc tcacttcgaa
     5701 gcgaaagcag cgctggatgc aggcgcgacg gaagctgaaa tgaagccaat tcaggacgat
     5761 atccgtcatg cccagtggcg ctgggatctg gcgatcgctt cccacggcat tcatatgcac
     5821 gcaccggaag aaggtttacg gatgctcggt acggcgatgg ataaagcggc ggatgcacgc
     5881 accaaactgg cgcgcctgct ggcgaccaaa ggcatcaccc atgaaatcca gatcccggat
     5941 atctcaacca aagagaaagc ccagcaggcc attggcctga acatggaaca aatcaaggcc
     6001 gagaagcagg acttcatcaa aacggtgatc ccgcagtggg aagaacaggc acgtaaaaac
     6061 ggtctgttaa gccaataacc ccgttccgcc tcgcaagggg cggaaaacac aatggagtga
     6121 atatgagcgt attacgttcg ttattaactg ccggggtgct ggcgtcgggc ctgttgtgga
     6181 gcctgaacgg aattaccgct acccctgcgg cgcaggcatc tgatgatcgc tacgaagtta
     6241 cccagcagcg taacccggat gccgcctgtc tggactgtca taaaccagat accgaaggta
     6301 tgcatggcaa acatgcttcc gttatcaacc cgaataacaa actgccggtc acctgcacca
     6361 actgtcacgg ccagccatca ccgcaacacc gcgaaggggt gaaagatgtg atgcgcttta
     6421 acgagccgat gtacaaggtt ggagagcaga acagcgtctg tatgtcctgt catctgccgg
     6481 aacagttgca aaaagcgttc tggccgcacg atgtccacgt aaccaaagtg gcgtgcgcca
     6541 gctgccattc cctgcatcca cagcaagata cgatgcagac gttaagtgac aaaggacgga
     6601 ttaagatttg cgtcgattgc cacagcgatc agcgcaccaa tccgaacttt aacccggcgt
     6661 ctgttccgtt gcttaaggag cagccatgac ctggtctcgt cgccagtttc tcaccggcgt
     6721 cggcgtgctg gcagccgtca gcggcaccgc agggcgcgtg gtggcgaaaa cgttgaatat
     6781 caatggggtg cgttacggca tggtgcatga cgagtcgtta tgcatcggct gtaccgcctg
     6841 tatggatgct tgtcgggaag tgaacaaggt gccggaaggc gtctcgcgcc tgacgattat
     6901 tcgtagcgag ccgcagggcg aatttcctga tgtgaaatat cgcttcttcc gtaagtcttg
     6961 ccagcactgc gatcatgcgc cgtgcgttga cgtctgcccg accggtgcgt cttttcgtga
     7021 tgccgctagc gggattgtcg atgttaatcc tgatctctgc gtcggttgtc agtactgcat
     7081 cgccgcctgt ccgtaccgcg tgcgctttat ccatccggtc acgaagacgg cggacaaatg
     7141 cgatttctgc cgtaaaacca atttgcaggc cggtaagctg cccgcgtgtg ttgaggcttg
     7201 cccgacgaag gcgctgacgt ttggcaatct ggacgatcct aacagtgaga tttcgcaact
     7261 gctgcgccag aagcctactt accgctacaa gctggcgctg ggaaccaaac cgaagctgta
     7321 ccgcgtaccg tttaaatacg gggaggtgag ccaatgacgc agacttccgc atttcatttt
     7381 gaatcgctgg tgtgggactg gccgattgcc atctacctgt ttttgattgg tatttctgcc
     7441 ggtctggtga cgctggccgt gctgttacgt cgcttctacc cgcaggcggg cggtgcagac
     7501 agtacgttgc tgcgcaccac gctgattgtc gggccgggcg cggtgatcct cggtctgttg
     7561 atcctcgtct tccacctgac aagaccgtgg accttctgga agctgatgtt ccactacagt
     7621 tttacctcgg tgatgtcgat gggggtgatg ctgtttcagc tctacatggt ggtgctggtg
     7681 ctgtggctgg cgaaaatctt tgaacatgat ttgcttgccc tgcaacaacg ctggttgccg
     7741 aagctgggga tcgtgcaaaa ggttctgagc ctgctgacgc ccgttcatcg cggactggaa
     7801 acgctgatgc tggtgttggc ggtgctgctg ggggcttata ccggctttct gctgtcggcg
     7861 ctgaaatcgt atccgttcct caataacccg atcctgccgg tgctgttcct cttctccggc
     7921 atctcgtccg gtgcggcggt ggcgctgatc gccatggcga tacgccaacg cagtaacccg
     7981 cattccacgg aagcgcactt tgttcaccgg atggaaatcc ccgtggtatg gggtgaaatc
     8041 ttcctgctgg tggcgttttt tgtcggtctg gcgctgggcg atgacggtaa agttcgtgcg
     8101 ctggtggcgg cattaggtgg cggtttctgg acgtggtggt tctggcttgg tgtcgccggg
     8161 ctggggctga ttgtgccaat gttgctcaaa ccgtgggtca atcgcagttc cggcattcct
     8221 gccgtgctgg cggcgtgtgg ggccagtctg gtcggcgtgt tgatgctgcg ctttttcatt
     8281 ctctacgccg gacagttgac ggtggcgtaa gccagaaaag aggtggtttc tggacgtatt
     8341 ccttcctgaa gtcggttttc tggcgttgtt gttaagtctc ggggtcaacg tgttgacccc
     8401 gttgacggcc tttgcgggag tgcggttgcg ctggcctgcc atgatgcgac tcacttgcat
     8461 cggcattctg gcgcagttcg cgctcctgct gctcgccttt ggcgtactga cgtattgttt
     8521 tctcatcagc gatttctcgg tcatttatgt cgcgcaacat agctatagcc tgctgtcgtg
     8581 ggaattcaag ctggcagcgg tgtggggcgg ccatgaaggt tcgctgctgc tttgggtgct
     8641 gctgctttcc gcctggagcg cgctgtttgc ctggcattat cggcagcaaa ccgatccgct
     8701 atttccgctg acgttagccg ttttatctct catgctcgcc gcactgctac tgtttgtggt
     8761 gctgtggtcc gatcccttcg tgcggatatt tccaccagca atcgaaggcc gcgatctcaa
     8821 tccgatgctg caacatcccg gtcttatctt tcatccaccg ctgctttacc ttggctatgg
     8881 cggtttgatg gtagcggcga gcgtggcgct ggcgagtcta ctgcgcggcg agtttgatgc
     8941 ggccagcgcc cgaatttgct ggcgctgggc gttacctggc tggagtgcat taacggcggg
     9001 gatcatcctc ggttcctggt gggcctactg cgaactcggc tggggcggct ggtggttctg
     9061 ggatccggtg gaaaatgcct ctttattacc ctggctttct gccactgcgc tgctgcacag
     9121 tttatccctg acacgccagc gggggatttt ttgccactgg tcgctgttgc tggcgatagt
     9181 tactctgatg ctgtcgctgc tgggcacctt aattgtccgt tctggcattc tggtttcggt
     9241 tcatgcgttc gcactggata acgtccgcgc cgtgccgttg ttcagcctgt ttgcactgat
     9301 tagccttgcg tctttggctc tgtatggctg gcgagcgcgg gacggtggcc cggcggtgcg
     9361 tttttcgggg ttatcgcggg aaatgttaat cctcgctacg ctgttgctgt tttgcgcagt
     9421 gctactgatc gtgctggtgg gaacgcttta tccgatgatt tacggtctgc tgggctgggg
     9481 acgcctctcc gttggcgcgc cgtattttaa ccgcgcgacg ttaccgtttg gtctgttgat
     9541 gctggtggtg attgtgctgg cgacgtttgt ctctggcaaa cgcgcgcagc ttccggcgct
     9601 ggtcgctcat gcgggcgtgc tgttatttgc cgcagggatc gtggtttcca gcgtcagccg
     9661 tcaggagatc agcctgaatt tacagccggg tcagcaggtg acgctggcag gatacacctt
     9721 ccgttttgag cgcctcgatc tgcaagccaa aggcaattac accagcgaaa aagcgatagt
     9781 ggcgctgttt gaccatcagc aacgcattgg tgaattaacg ccggagcggc gtttttatga
     9841 agcacgccgt cagcaaatga tggaaccgtc aattcgctgg aacggcatcc atgactggta
     9901 tgcggtcatg ggtgaaaaaa ccggagcgga tcgttacgct tttcgtttgt atgtacaaag
     9961 cggtgtgcgc tggatctggg gtggaggatt gttgatgatt gcgggcgcat tgttaagcgg
    10021 atggcggggg aagaagcgcg atgaataaag ggcttctcac gctactgctg ttatttacct
    10081 gttttgcgcg cgctcaggtc gtagacacct ggcaattcgc caatccgcaa caacagcaac
    10141 aggcgttaaa tattgccagc cagttacgtt gtccgcagtg ccagaatcaa aacttactcg
    10201 aatccaatgc gccggtggcg gtcagtatgc gccatcaggt ttatagcatg gtgtcggagg
    10261 ggaagaacga agtcgaaatc attggctgga tgaccgaacg ctacggagat tttgttcgct
    10321 ataacccacc gttaacgggt cagacgctgg tgttgtgggc gctgccagtg gtgttgttat
    10381 tgctgatggc actgatcctc tggcgagtga gggctaagcg atgaaacagc ctcaaatacc
    10441 ggtgaaaatg ctgacaacac tcacgatatt gatggtattc ctctgtgtcg gcagttatct
    10501 gttaagccca aaatggcagg cggtacgtgc ggagtatcag cgtcagcgcg atccgctaca
    10561 tcagtttgcc agccagcaaa ccccggaagc gcagcttcag gcattgcagg ataaaatccg
    10621 cgctaatcca caaaacagcg aacaatgggc gttactgggc gagtactatc tgtggcaaaa
    10681 cgattacagc aattcgctgc tggcgtatcg tcaggcgttg caactacgtg gtgagaacgc
    10741 tgaactgtat gcggcgctgg cgacggtgct ttattaccag gccagccagc atatgaccgc
    10801 ccagacttgc gcaatgatcg acaaagccct cgcgctggac tctaacgaaa tcaccgctct
    10861 gatgctgctg gcttccgatg cgtttatgca ggcgaattac gcgcaagcca tcgaactatg
    10921 gcaaaaagtg atggatctca actcaccccg gatcaaccgg acacagctgg tcgagtcgat
    10981 taatatggca aaactgttgc agcggagatc ggattaatca tctggtgatg aagattatgt
    11041 cattgagctg cataaaaaat aatcgaatga acatatgcca aaaataatca ctaatcagta
    11101 ttattgcaga ttaaacaaat aaaaatcttt ccataacaaa tggttattca ttaatcctgc
    11161 cataaactcg cgttattatg cattaatgca gcgaaaagct ctgttgttaa agggttgcgc
    11221 aacataccgc gcaaatgata ctgatcataa gcgttaaaaa aatctacaaa ccaacgcaac
    11281 acaattcatg ccctggcagt atgtcacgtt ctcgcgtttc tgaacgggga acggcgctcc
    11341 attgaggaag ttattctgat gaaaaatata aaattcagcc tggcctggca gattctgttt
    11401 gctatggtgc tgggcattct cctgggaagc tacctgcact accatagcga cagccgcgac
    11461 tggctggtcg tcaatttgct ctctccggcg ggtgatatct tcatccatct gatcaaaatg
    11521 attgttgtgc cgattgtgat ctccacgctg gtggtgggta tcgcgggtgt tggtgatgcc
    11581 aaacagctcg ggcgtattgg cgcgaaaacc attatttact tcgaggtgat caccaccgtc
    11641 gccatcattt tggggatcac tctggcgaac gtcttccagc ccggtgccgg ggtggatatg
    11701 tcgcagctgg cgaccgtcga tatctcgaaa tatcagagca ctacggaagc ggtacaaagc
    11761 agttcccacg gcattatggg cacgattttg tcgctggtgc cgacgaacat tgtggcgtcg
    11821 atggcgaaag gcgaaatgct gccgatcatc tttttctcgg tgctgtttgg tctggggctt
    11881 tcttccctgc ccgcgacgca tcgtgaactg ctggtgaccg tgttccgctc catctctgaa
    11941 accatgttta aagtgactca catggtgatg cgttatgcac cggtgggtgt gtttgcgctg
    12001 attgcggtga cggtggctaa ctttggtttc tcttctctgt ggccactggc gaaactggtg
    12061 ctgctggtgc atttcgccat tctgttcttc gcgctggtag tgctgggaat tgtggcgcgc
    12121 ctgtgcgggt taagcgtctg gatcctgatt cgtattctga aagatgagct gattctggcg
    12181 tactccactg ccagctctga aagcgtactg ccgcgaatta ttgagaagat ggaagcctac
    12241 ggagcaccgg cgtcgatcac cagcttcgtg gtgccgaccg gttactcttt taaccttgat
    12301 ggttcgacgc tgtatcaaag tattgccgct attttcatcg ctcagctgta tggcattgac
    12361 ctgtccatct ggcaggaaat cattctggtg ctgacgctga tggtgacctc gaaagggatt
    12421 gctggcgtgc cgggcgtgtc gtttgtggtg ctgctggcaa cgctgggtag cgtaggtatt
    12481 ccgctggaag gtctggcgtt tattgctggt gttgaccgta tcctcgacat ggcgcgtact
    12541 gcgctgaacg tggtgggtaa tgcgctggcg gtgctggtga ttgccaagtg ggaacacaaa
    12601 tttgaccgca aaaaggcgct ggcttatgag cgtgaagtgc tgggcaaatt tgataaaact
    12661 gcggatcaat aattgaagat tgccggggat atccacccgg caatgtgtga atgcctgatg
    12721 cgacgcttgc cgcgtcttat caggcctacc ttaaccgttg gttaattttt caaactcttc
    12781 acacccggta tcaaaccctt ccatacagct caggttcaac cagtgcagcg ccttttgctt
    12841 gttcttctcg ataaaacctt cttcaccgtt taagaacatc attcccgccc agtactcgga
    12901 ataaccggtt cgggaaattg cagagctgcg tttgaaatac caggttgcct tgtcatcgtc
    12961 ggccttaatg cccacgccgt tggcgtaaat caaaccaagc agcatttggg catcgacggc
    13021 agagtcgttc tccagatctt ccgaggcgtt ttctaacagc gaaatagcct ttggataatc
    13081 cggtttaccc gcttgagtat ttaccagaat atgcgccagc gttacttcac cttctttact
    13141 gcccgcttgc gccgctttct ctgcaagaac ttttgcttgt ggatagtcca gactgaccgg
    13201 attggtgatt ttgatctgcg ccagcagcgc gcaggcatca gcatcaccgc tgtcggcggc
    13261 tttctgtgcc caatattcgg ctttgctcaa atcgccggag ctaaaccagc tgtcggcaag
    13321 aaaatattgt gcgcgtcggt ccccggcctc tgctgccttt aaatactggc tgccaggctc
    13381 ggagtcgttg gcgtgggcaa agaatgtcaa aaacaacatc aatgcgataa tttttttcat
    13441 tttgataata cgattgggta gggagtaacc agtataaaga gttcgaatgg ataaaaaaac
    13501 agcctccgaa aggaggctgt agaaaggact gtattacgcc agtgccgctt cgcgcaggcg
    13561 agttttcaac ttgttgtact cgtcaatcac gtactgctcg gcggcgcgct gatcggcgat
    13621 cggctcgacg cgcacggcgc agtatttgta ttccggcgtt ttcgtaatcg ggcttaagtt
    13681 ttcggtaacc agctcgttac aggcaccaat ccaccactgg taggtcatgt aaatcgcccc
    13741 tttgttcgga cgatcgctga cctgcgcacg ggtgataatt ttgcctttac gcgagtgcac
    13801 ccaaaccaat gcctcatctt caatacccag acgtttggcg tcttcggtat tgatttgtgc
    13861 gtagccaggt tcatcagcca gcgccgccag tgccgcacag ttaccggtca tcgaacggca
    13921 agagtagtga ccaacttcac gcaccgttga cagtaccatc gggtactcgt cggtgagttt
    13981 gtcgattggc gctacccagt cgcaggtgaa gaactgcgcc agaccgttcg gggtatcaaa
    14041 cttctcttta aacagataag aagtcccctg atcggcatct gaagtatcgc ggcaaggcca
    14101 ctgaatgaag cccagttcgc ccattttctc gtaagtcgca ccgtagaaat ccgggcacag
    14161 atgacgcaac tcatcccaga tctcctgggt gttgttgtag tgcatcggat aacccatacg
    14221 ggtggcgatt tcactgatga tttgccagtc cgttttcaga tcccatttcg gttcaactgc
    14281 tttgaagaaa cgctggaagc cacggtcagc cgcagtaaac acgccttcat gctcgcccca
    14341 cgacgttgac ggcaaaatga catccgccgc cgacgcggtt ttggtcataa agatgtcctg
    14401 aacgataacc agttccagat cttcaaaggc tttacgtact gccgacagct ccgcgtcagt
    14461 ttgcagcgga tcttcgccca taatgtacgc ggcacgcact tcgccatgcg ctgcgcggtg
    14521 cggcagctcg ctgatgcgat aaccggtatg agcaggcagg ctttccacgc cccaggcttt
    14581 ggcgaatttc tcgcggttag ccggatcttt cacgtactgg tatcccggat acgtatccgg
    14641 cagcgcgccc atatcgcagg caccctgcac gttgttctga ccacgaaccg ggtttacacc
    14701 cgcatgcggc ttaccgaggt taccagtcag catcgcgagg ctggtcagag aacgcacggt
    14761 ttccacgccc tggtagaact gggttacacc catgccccac aggatggcgg cgcttttcgc
    14821 ctgggcatac atccgtgccg cctgacgaat ctcactggcg ctgacgccgg tgatatcttc
    14881 aaccgactcc ggcgtgtagc cttcaacgat tttacgatac tcttcaaagc cttctgtacg
    14941 ggaagcgacg aacgctttgt cgtacagatt ttcttcaata atgacatggt ccatcgcatt
    15001 caacagcgcg atgttcgagc cgtttttcag tgcaatgtgc atgtcagcaa tgcgcgcggt
    15061 ttcaattttg cgcggatcgc agacgataat tttcgccccg ttacgtttag cgttaattac
    15121 gtgattcgcc acgattgggt gggaatcagc cgggttgtac ccgaaaacga acactaaatc
    15181 ggtattatca atttcgttaa tagcattgct cattgcgcca ttaccgaccg attggtgcag
    15241 acctgcaacc gatgggccgt gtcagacacg agcgcagcag tcaacgttat tggtaccaat
    15301 aacggcgcgc gcaaattttt gcattacata gttggtttcg ttacccgtac cacgcgagga
    15361 gccggtcgtc tggatggcat ccggaccgta cttctctttg atggcgctca ggcgctcggc
    15421 aacgtaattc agtgcctcat cccaggaaac aggttcgagt ttgccgccac gctggcgacg
    15481 gatcatgggg gttttcaggc gcggggtcag gatctgggta tcgttaatga aatcccagcc
    15541 ataataaccc ttcagacaca gggtaccctg gttggttttc ccctgcgctg cctccgcccg
    15601 gacgattttg ccgttatcga cgaccaggtt gattttgcaa cctgatgcgc aataggggca
    15661 aaccgtgacg acttttttca tcggtctcgc tccagttaat caaatcacgc atacgcgctc
    15721 tcgactacag tatgcatctt ttatgccaca ttttatttgg ggtcattccc tgatattacg
    15781 ggcactattt attcaaaact ctgacgaaaa acaggctgtc gtcagttttg acgtgacgaa
    15841 acgaaatacc gcgtgacagc catcacgcgg cagacatttt attttttctc gacgacggga
    15901 gccgcctgat acccgccgcc cagcgatttc atcaactgaa tgctttggat cacccggcgg
    15961 ctgtccagca tcagtaatga catctcttcg gcaagtactg gcaaccgggc ttcagtggcc
    16021 tgtaagcggc tggttaagcc gcgctgatag gcggcctcgg cagcgcgctg ggtgaagcgc
    16081 gttgcttcca cgcgttcagc ctgcatttct cgctcgtcgt tgagcgtttg cagacgcgtg
    16141 ccgttgacgg caacgtcacg caccgcgttc agtactgact ggttgtaacg ttcaatcatc
    16201 atgttgctgg cggcgcgcgt gccttcgaga ttggcattca accgtccacc gtcaaacagc
    16261 ggcaatttca gacccgggat gaagttgaac tggcgactgg tttttttgaa taaggtatcc
    16321 aggtggatgg catcaaggcc gaaaaacgct ttgatatcaa agctcgggta gaacagcgcc
    16381 cgcgcggaat ccacctgatc taatgacgcc tgaacatacc agcgcatggc ttgcagatcc
    16441 gggcgtctgg cgagcaactc ataagagagt gtcgccggaa tgccggtctg gactcgtggt
    16501 aatgccaccg gtttgatctc cggcatatcg ctggcccccg cgccaatcaa tgcacgcaga
    16561 gattctcgcg tttcggtgat ttgccctttg acggcagcaa tttgtttatc gaccgccaga
    16621 atctgtgccc gcgcgccgtg gaaaggcact tgtgcttcca gaccgtgcgc cactttactc
    16681 tggtgcgctt tcaccgcgta atcaatcaca tcgcgagttt gttctaacag atcgagcatc
    16741 tgatagctgg cctgcatact gtaataaagc tgcgctacgc ccgtggtcag cgatagctct
    16801 actgctgcgg tttctgccag cgcggcatta tgcgcgccaa tggcggcggc aaccgctgag
    16861 cgatgcacac cccacaaatc aagatccagt ccggcaaaca aacctactgt ggcttccgta
    16921 tagtacggcc cgtccatacc cagtgcgggc gcatccatcg cataagggct taaaaagccg
    16981 ttcgccgaga cgcgttggcg gttgagcatc cctaacgctg cgacctgtaa ttgtgaaccg
    17041 gcatctaaca aatcggcctg ggactgcgct ttttcttccc gcagtttcgc ttcggcgagg
    17101 gtgtgtgaac cacttagcgt ccgttggatc agcgcatcca gctacgggtc attgagttgt
    17161 ttccaccact gcgcctgcgg ccagccggag ctggcaagat gaatatcatc ggccagtttg
    17221 atttgttccg gtttgagctg ttgatgaggc gcagaatcct tgcgtaccaa ggcacagccg
    17281 gaaatcagcg tcgtgctgcc gagaatgctg cacaacagca gacgtgaaag ttgacgattg
    17341 atcattgcgt ggctccttgt gcctgttccg tggcgggcgt taatgccggg gctgtccagt
    17401 ctggcaagcg ggcgaaaagc tggcagacgt gttgctcctg ttttaataag gtgggcagga
    17461 tggcctgcga ggcgggtgtc tcttcaagcg ttatttgagg agaacggctg agtgcggttg
    17521 ccagaccggc agcgtatttt tccagggcgt cggcaaactg acctgctctg tccggctgta
    17581 gtgcgttatc cagcgcctgc gccgaattcc aggtggcatc ccaggcgtga aggatatcgc
    17641 ggccctgacg aataaccgtt tgcgaacgtt caatcagtaa ggcgcgttct tcgctgtcca
    17701 gttgacgctc cagcgccacg cgttggcaca tctcttcaca ggcattaaac gccgcatgca
    17761 gaccgatacg aatttgcaga taagtgcgca gagccgtgac ttcctgctgg cgtgggatcc
    17821 gcattacttt acttaacata cccagcgcgc cagccagttt ttgcggcagc gtgcgcgctt
    17881 cactttcagg ccagacaaag gtgtaaatca ccgcggacac cacggtacca atgatgatac
    17941 ccagggcgcg atcgcgaatt tccaccaggt cgtacactgg gccaaaaacg ttttcgagcg
    18001 tggcgagcgc gaaggtgacc accatctgtg tgccgatata agaagagcgt tcagagctgg
    18061 tggcaatcca tgcgcccaac aggaaaatcg gtgccagcac aaacagcaat tcgacaatat
    18121 tgtccagcca gggcatgacc agtagcgtga ataacagcgc cagaatcgcg ccgcaaaagg
    18181 ccccgccaaa acgcagcacc atcttctggt acgacgaacc gacatttgga ttagagacga
    18241 tcacgcaggt cagcatacag gtgtgaatgc cttcccagtc cacgccgctg taaaaggtgt
    18301 aacagatcaa acaggcgagc aatgttttta ccgcgtagcg tatatagtct ggattggtaa
    18361 aagcatcggc gaccattgat ggcggtttgg cggcgggcgt tggcggcgta ttcgggttca
    18421 tctggcccag ttgtaacaac gtctggcaga tattctccag gttacattcc cgtgccgcca
    18481 ccgcttcact ttcactgatc cgccagtcgc tttgccagca ctgaccttca gcaacggcat
    18541 gctgcagctt gttgatttct gaggctaatt tttgccgaaa ttcaataatt gcctgagaat
    18601 cagcaaaaga ggtgggatcg tagcgattca gcgtcgagta aatgtaggtt accgttgcca
    18661 cgcagctttg ccaccatgcg ctttgagttc gccagttggc atcgtccgcg agacaaaaga
    18721 cattcagttt ttgcagcgcc agcgcctctc tttcaatccg cgtttcgggt agcggtgcga
    18781 ggctgtctgt caggtgacta atggcatcat caagccgatc attaagcgcc tgatgcattt
    18841 gcgaaatggc acgactggga aaccacagca cgccgattaa cgtcatcagc aaggttggat
    18901 agaggccaac aacgatacac cacagcgtta agcgcacgac cacttccgga tagtcgagca
    18961 tggcggggaa ggtttgcccg taaatggcga caatggcgac agcgaaaaag accagtccca
    19021 gccgatgggt gcgcatcaaa aacatgcggc ccatcaggat cggcccggcg atgatcaatc
    19081 ggatcaacgg ttcgccgtat gaccatttgt agatcaaaaa caggctgccg atctccagca
    19141 ccgtggcaac cacaaacaag atcgccacaa atttggtgta aaacgcgttc gactgaatac
    19201 cgtaaaacag cactgccagc gataacgcca caaaagggat ctcaaaagtc atcgagatca
    19261 gaatcaccag caggcagcct acccagagtt gcacgatctg cggcacgcgg ccgggtcgcc
    19321 gctcgcttaa ctcttcatga aagaacgcca gcagcctgac catcggtaat ggcagggagt
    19381 tgagcgcgct cattattgcg gctcaagatt agcgactgcc gaagcgccga tgcggaacat
    19441 ttccgggtca ggtttatcga ccatgatttt gaccggaaaa cgctgggcaa cgcggaccca
    19501 gttaatagag cgagaaactt tcggcaggcc gcccagcacc aggccgccgt catccggtag
    19561 cacgccgtag ccaatcgaat ccactttacc ctcgaaggtt ttgccgctgt cactcatcag
    19621 gcgaatcgtt gcgggtgtac ctgagcgaat atttttcaga tcggtttcgc ggaagttggc
    19681 gatcacgtac cagtgacgag tgtcgattag ggtaaaaata gggcgcatgg cagaagcaaa
    19741 ttgcccgacg gaggttttga gggaaatgac ccggccatca aacggcgcgc gaacggtcgc
    19801 catttccaga tgcagtttgg tcagggcaat atccgcttcg accgccgcac gttgcgccac
    19861 cagcgcatcg acgccgctga cggcgctggc ggctgactgc gcctgtaaca atacggcatt
    19921 aagatcggct tctgcggcgc gctgcgcagt tcttgcacgg tcaacgtctt ccgctgagac
    19981 aaaaccctct ttcagtaatg gctcggtacg gcgtaatgta tctgtggcct gtttcgcggc
    20041 ggcacgggct ttttctaccg tggcattaat cgagtcggca ccaaactgtt gcgcatcaac
    20101 gctacgctgg gtgagcataa tttgcttatc cagcgccgcg agggaggctt cagctttcgc
    20161 cagattggct tcgtacgggc gcgggtcaat gcggaacagc aaatcgccct gtttgactgc
    20221 ctggttgtcg gtgaccgcca gttctacaat gcggccgctg acttccggca ccacatcaat
    20281 ggtatctgct gacgcgtaag cgtcattagt tgatggcgca ctgtctacgc gccagataac
    20341 gaaaacaagg gcaaccagcg ccaatgcaac cactaacaga gcagggaatt tactgcgagg
    20401 agcttttttc ggcgtacttt ccatgagcgc gtctcaaata gattagaaaa atgccagcca
    20461 gaacagcatg gcgtagagag caaaaagggc ggtataaata attccgacaa atgccagatt
    20521 gatatttgcc cgctgaataa ctcgcctcgt gatgagcatc aaaataaggc tggcaatggc
    20581 gcagaaaaac cagctgggat aataagcacc gatcaccggg attgccggag ataatgagca
    20641 tcccgaaacc atgaagacgg gaattatcca ggctgttttt ttgagttgca tcgccatgcg
    20701 agagagaact gttggcatat taaatacatc ctgtctgctg cgaacaatat ctgtagtgaa
    20761 tatcaatatc cattgaaggc gtcagtatgg ctattttctc gatgaataaa aatagcttta
    20821 ttcagtc
//
LOCUS       DAZPDU010000066        20403 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZPDU010000066 DAZPDU010000000
VERSION     DAZPDU010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20403
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.067"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..348
                     /gene="ydjO"
                     /locus_tag="ACHURS_002190"
     CDS             <1..348
                     /gene="ydjO"
                     /locus_tag="ACHURS_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjO"
                     /protein_id="HFR1237913.1"
                     /translation="AFLLRFKKNESLLKFPSIISMLNINLLFNPLNLPGMGSGVLEDI
                     MSIPDSSLRKRLGYEVLSFSLQAHSLSQECIDKLDIFFADDLFKYESVCIAAMEHLKS
                     KATAPIQNGPLPA"
     gene            complement(352..1743)
                     /gene="tcyP"
                     /locus_tag="ACHURS_002191"
     CDS             complement(352..1743)
                     /gene="tcyP"
                     /locus_tag="ACHURS_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFR1237914.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(1876..2466)
                     /locus_tag="ACHURS_002192"
     CDS             complement(1876..2466)
                     /locus_tag="ACHURS_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR1237915.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2629..3297)
                     /gene="hxpB"
                     /locus_tag="ACHURS_002193"
     CDS             complement(2629..3297)
                     /gene="hxpB"
                     /locus_tag="ACHURS_002193"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFR1237916.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3444..3980
                     /locus_tag="ACHURS_002194"
     CDS             3444..3980
                     /locus_tag="ACHURS_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFR1237917.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(4021..4881)
                     /locus_tag="ACHURS_002195"
     CDS             complement(4021..4881)
                     /locus_tag="ACHURS_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFR1237918.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQLQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4987..5277)
                     /gene="ghoS"
                     /locus_tag="ACHURS_002196"
     CDS             complement(4987..5277)
                     /gene="ghoS"
                     /locus_tag="ACHURS_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFR1237919.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5378..6307)
                     /gene="pfkB"
                     /locus_tag="ACHURS_002197"
     CDS             complement(5378..6307)
                     /gene="pfkB"
                     /locus_tag="ACHURS_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFR1237920.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISASQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            6594..7352
                     /locus_tag="ACHURS_002198"
     CDS             6594..7352
                     /locus_tag="ACHURS_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFR1237921.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(7405..7512)
                     /gene="yniD"
                     /locus_tag="ACHURS_002199"
     CDS             complement(7405..7512)
                     /gene="yniD"
                     /locus_tag="ACHURS_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFR1237922.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(7684..9582)
                     /gene="espL1"
                     /locus_tag="ACHURS_002200"
                     /pseudo
     CDS             complement(7684..9582)
                     /gene="espL1"
                     /locus_tag="ACHURS_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            10107..12035
                     /gene="thrS"
                     /locus_tag="ACHURS_002201"
     CDS             10107..12035
                     /gene="thrS"
                     /locus_tag="ACHURS_002201"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFR1237923.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            12039..12581
                     /gene="infC"
                     /locus_tag="ACHURS_002202"
     CDS             12039..12581
                     /gene="infC"
                     /locus_tag="ACHURS_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFR1237924.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            12678..12875
                     /gene="rpmI"
                     /locus_tag="ACHURS_002203"
     CDS             12678..12875
                     /gene="rpmI"
                     /locus_tag="ACHURS_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFR1237925.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            12928..13284
                     /gene="rplT"
                     /locus_tag="ACHURS_002204"
     CDS             12928..13284
                     /gene="rplT"
                     /locus_tag="ACHURS_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFR1237926.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    13401..13525
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            13407..13451
                     /gene="pheM"
                     /locus_tag="ACHURS_002205"
     CDS             13407..13451
                     /gene="pheM"
                     /locus_tag="ACHURS_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFR1237927.1"
                     /translation="MNAAIFRFFFYFST"
     gene            13734..14717
                     /gene="pheS"
                     /locus_tag="ACHURS_002206"
     CDS             13734..14717
                     /gene="pheS"
                     /locus_tag="ACHURS_002206"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFR1237928.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLLGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            14732..17119
                     /gene="pheT"
                     /locus_tag="ACHURS_002207"
     CDS             14732..17119
                     /gene="pheT"
                     /locus_tag="ACHURS_002207"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFR1237929.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            17124..17423
                     /gene="ihfA"
                     /locus_tag="ACHURS_002208"
     CDS             17124..17423
                     /gene="ihfA"
                     /locus_tag="ACHURS_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFR1237930.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            17524..18504
                     /gene="btuC"
                     /locus_tag="ACHURS_002209"
     CDS             17524..18504
                     /gene="btuC"
                     /locus_tag="ACHURS_002209"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFR1237931.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASAVLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            18567..19118
                     /gene="btuE"
                     /locus_tag="ACHURS_002210"
     CDS             18567..19118
                     /gene="btuE"
                     /locus_tag="ACHURS_002210"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFR1237932.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            19118..19867
                     /gene="btuD"
                     /locus_tag="ACHURS_002211"
     CDS             19118..19867
                     /gene="btuD"
                     /locus_tag="ACHURS_002211"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFR1237933.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            19944..>20403
                     /locus_tag="ACHURS_002212"
     CDS             19944..>20403
                     /locus_tag="ACHURS_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFR1237934.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARR"
BASE COUNT         5257 a         4771 c         5206 g         5169 t
ORIGIN      
        1 gcatttttgc tgcgattcaa aaaaaatgag agtctcctaa agtttccttc gataatatca
       61 atgcttaata tcaatcttct ttttaatcca ctaaatcttc cgggaatggg aagtggtgtg
      121 cttgaagata ttatgagtat tccagatagt tcacttagaa aacgtctcgg gtatgaggtt
      181 ttaagcttct cactacaggc acattcattg agccaggaat gtattgataa gttggacatt
      241 tttttcgctg acgatctgtt taaatatgag tcagtatgca tcgccgcgat ggaacattta
      301 aaaagtaaag caacggcccc tatacaaaac ggaccgttgc cagcataaga attaacggtg
      361 tgccagttcg gcgtcgtctt cgctatccag aatggcttta tcggtttgct tcagccactg
      421 gctggtcagc gtgccagctg tcatcgagcc actaacgttt agcgccgtac ggcccatgtc
      481 gataagcggt tcaacggaga ttaacagcgc caccagggtt actggcaggc ccatcgcagg
      541 cagcacaatc agtgcggcga aagttgcacc accaccgaca ccggcaacgc ctgcggaact
      601 aacggtaaca ataccgacca gcgtcgcaat ccacatcggg tccagcgggt taatgccaac
      661 cgtaggcgca accatcaccg ccagcattgc cggatacaaa ccggcgcagc cgttctgacc
      721 aatggttgca ccgaaagagg cggcgaaact ggcgatggat tcaggaacgc ccagacgacg
      781 cgtttgtgct tccacattca gtgggataga cgcagcactg gaacggctgg taaaggcaaa
      841 cgtcagcaca ggccatacct tacggaagta cttcagcgga ctcacgccat taatgcccag
      901 cagaatgcca tgcactgcaa acataatcag cagaccgagg taggacgcga caacgaaact
      961 tcccagtttg atgatgtctt gcaagttaga acctgcaacc actttggtca ttagagccag
     1021 aacgccgtaa ggggtcaact gcatgacaag gcgaaccagt ttcatcaccc agctttgtag
     1081 ggtatcgata gcggctaaga cgcgttcacc tttcggcgca tcatccttca gcagtttcaa
     1141 tgcagctacg ccgaggaatg cggcaaaaat taccacgctg ataattgacg tcggattggc
     1201 tccggtaaga tcggcaaacg ggtttttcgg gataaaggac aagaccagct gcggaacgct
     1261 cagatcagag actttaccaa catagttact ttcaatggcg ttcagacgtg cagtttctgc
     1321 accaccctga accagacctt cagccgtcaa accaaacagg ttggtgacca gcacaccgac
     1381 cagcgccgca atcagcgtgg taaacaaaag cgtaccgatg gtcagaaaac tgattttgcc
     1441 taactgagat gcgttatgca gacgggcaac cgcgctcaga atagaggcga agactaacgg
     1501 cataacgatc atttgcagca gttgaacata gccgttacca acgatgttaa accactgtac
     1561 agaatcttta agtacctggc tgtcagaacc ataaatggta tgcagggcaa ggccaaaaac
     1621 cacacccatc accagaccca ccagcacttt tttcgccaga ctccactgtt tatggcgggt
     1681 ctgagccagc gcaaacagca gtacaacgaa caccacgatg ttcgcaatta atggaaagtt
     1741 catccccgtt ctcctgatct tattatttct atcactgcgt agcagtcata gtgttggcgc
     1801 aagattaaca gaactgtgag ctggctctta tattcaaatg gaattgctta ttccagaaag
     1861 gtgatttttg cctttttagt attcaacctg atgcttaata agccgatgaa actggatctg
     1921 caaggtattg atcatttgcg atgaccaacg aacagcgctg ttctcgggta atgaatgggg
     1981 catccatacc gaccagacaa ataatgccat gcagataaaa cgttccagtt ggttgccctt
     2041 ttggggaacc aggataggca agcggaaacg ccagcggcat ggccagagca ggggaacacc
     2101 ggcgggtgtc agcatatcgg caagtatgtg gctcaaataa cccagcacca ttccttgtag
     2161 cgcatcagcc ggaatgaacc agccttccgg aaccttaagg taaaaggttg ccagcagcgc
     2221 aaataccgcc agcagactgt gggtaaaacc acgatgccca aaagcgcggg cgatcggttt
     2281 tgatatccat tttaatcgct gcccaagaaa cgactttggg tgatcgatgt ccggtaacaa
     2341 acacgtcagg attgcggaag ggacaatatg ccaccagtca ccctgtgcca gcacgggcgt
     2401 cagctcggca tttttggcaa ataccgcaca agcaatagaa aagagaaggt gaccttccgc
     2461 cgtcatgata aaactcacta aactgtcgat acgtacagta taggattttt atacagtgca
     2521 cggaagtggt gacggtgtaa ctctttctct taaattgacg attaatccgt cagttgaatg
     2581 caaattcaga taaagaacca ggggcagcgt gatcactgcc cctggtcatt aaccgagaag
     2641 gtcttttgcg gtgagttctg tcagcgatga gagtttgacg tttgctaata caaaacgtgg
     2701 atcattttgc gcttctggcg caggaacgac gatggaacgc atgcgggctg ctttagaagc
     2761 gatcatgcca tttaccgagt cttccagcgc cacgcaggtc agtgggtcaa cgcccagttt
     2821 tgctgcgcag tcgagatata cttgcggatg cggcttgctg taaggcagtt tttcagccga
     2881 ggcgagggca tcgaaactgt cgcgtaagtc aaacatggtc aacacttttt ccagcatatg
     2941 tagtggtgac gcggaggcca gtcccaccaa taaaccttgt tctttgcata acgcaacagc
     3001 ttcgcgcacg cctggtaata atggacgtgt ctcttcaacc agtgaaatgg cacgggcaat
     3061 aacccgttct actacttcct gacggcttgg cccattccac ggttgccggg cgtaccaaag
     3121 atcgaccacc atatcgatgc gtaaacctaa ggtgtccggc agctcgttac gacgggagat
     3181 atccaccccc aggcttgcca tcacatccag ttcggctcga tcccataaag gttctgagtc
     3241 gataagtaat ccatccatat caaaaattgc agcaagaatc tgacgcgggg ttgacattac
     3301 aacctctcct ttagagcatt atgtgtaagg tattaagccg tgcttatgat taagcgccta
     3361 tcatatcgcc tttaaagaat gggcgaaaac agatatctac aggtaaacta agtgaaacga
     3421 cgcgtcttaa tcaaggagaa ctcatgacgt atcaacaagc tggacgcatt gctgttttga
     3481 aaaggatttt gggttgggtg atttttatac ctgcattgat ctccacattg atttctttgc
     3541 tgaaatttat gaatacccgg caggaaaacc aggaaggcat taatgcggtc atgctcgact
     3601 ttactcatgt catgatcgat atgatgcagg cgaatacgcc ttttttgaat ctgttctggt
     3661 ataactcccc gacccctaat tttaatggtg gcgtgaacgt tatgttctgg gtgattttca
     3721 tcctgatttt tgtcggactg gcgttgcagg attccggtgc ccggatgagt cgccaggcgc
     3781 gcttcttgcg ggaaggtgtt gaagatcaac tcattctgga aaaagccaaa ggggaagagg
     3841 ggctaacgcg tgaacaaatt gagtcccgta ttgttgttcc gcaccatact attttcctgc
     3901 agtttttctc actatatatc ctgccggtta tttgtattgc tgcaggttat gtgttctttt
     3961 ctctgcttgg gtttatttaa tcaaaaggcc gcacggagcg gccattatcc tcaacccata
     4021 tcatgctgct aataatctat ccaatgactg ctgagcaata accaaatgct gaccgccaaa
     4081 taatcttgca cgatttaaca gcgtgtagag ttggtaaacc ggttgacgtt caaggaaatc
     4141 ggcaggtagc ggggatactg actgatagcc gtcatagatt tgtggcggct gttcagtatg
     4201 cagcggtaac atcgccaggt cgcactctcg gtcaccccag tagcaggcag ggtcgaaaat
     4261 gtacgggcca tccggaccca gcgcacagtt gccggaccat aaatcgccgt gcaacagaga
     4321 aggctgcagt tgatgcgagg ccagacgctg ctgaatatgc tcgacgatgg cgtcgatatt
     4381 gccgaaagcg atccctttct ctgctgccag ttccaactgc cagccaatcc gttgttcagc
     4441 aaaaaacgtt gaccagcgac gttgccaggt gttgggctgt ggagttgtgg agagcgagtt
     4501 atcaaaatcg aggccaaatt gtggttggtc actccattga tgtaaacgcg caatttgctg
     4561 accaagaata aatgcgctat gcgcatccag tggacgaggt gggagataat ccatcaccag
     4621 aaaactgtag tcacggtcag cgcctactgc ccaaacctta ggcacggtga cggttttact
     4681 acgcgacagt aactccagtt ggtcggcttc ggcggtaaaa ccgggaagca gttccctttc
     4741 atcacatttg acgaaaaagt catgtcctgc atagcgcaaa tgccatgcgg catggacttc
     4801 tccgccaggc agttcattac gcagttcgat ttcgccttca cctaactgct cgctcaaaag
     4861 acgactgatt gcctgccaca tgatgtctct cccatgttgt ctgccagatc ataaggttag
     4921 cgcaagaatg cggtgtaaaa acacgagcta acgcacacca cagactgatt attgcccaaa
     4981 aaccatttat tttctgttgc tattccattc ctcccaagtc gtgatggtga tttcaggatc
     5041 tttgccggtt gcactttggg taagccccga aaccagttct ctaatttctt cttcactttg
     5101 ggtactgata agtccaaaag tgttagtccc cagctcatgg atatttccct catcatccgt
     5161 catggtgagc aaaaaaccgt cgcgagtcag gtaattattc aactcgttaa tttcagtcaa
     5221 tgtatcctca tgcaacatga cggttatgac ataacggaca agatcgccac tggccatagt
     5281 tcaccccttt gttatcacat gacttttgag caaagtcgga gaaacgcgtt gccgacaggt
     5341 tggtggtgat tcccccaatg ctgggggaat gtttttgtta gcgggaaagg taagcgtaaa
     5401 ttttttgcgt atcgtcatgg gagcacagac gtgttccctg attgagtgtg gctgcactcc
     5461 ccgcagctac gccaaaacgg accatctctt caagagaggc attttctgcc agtttcagtg
     5521 tcatcgcgcc gaccatgctg tcaccagcgc caacggtact ctggcttttc accggtggtg
     5581 gcaccacctg aatgcagttt tcactatcaa cacccagcgc tccttgtgga ccaagggaaa
     5641 cgacaacccg tttggccttg ccgctattaa cgatttcctg cgcggctttg cggacatcgt
     5701 ccggctgggt gagttcgcga ttcaccagcg cactgagttc tttttggtta ggcttaacca
     5761 actcgatgtt accaattgcc agtgctgcac ttaacgcttc gccagaactg tcgacgatgc
     5821 agcggatccc ttgtttttgc gaagcggaaa tcagttgggt taatttttcc agcttcacac
     5881 ctggcggcag gcttccgctt atgaccagga tggccccgga ttcaatttcc agaacttgct
     5941 cttcaagctg gcgaaactca tcttcattta atgccgcgcc tggcataaca aaacgatact
     6001 gctcaccgct tgcttccaca tgtacatgta aattctgccg ggtccagtct ttggcttcta
     6061 cagtagcgac ggggacattt tcatccgcca acagtgaaac caggtgttcg ccggtcgcgc
     6121 cacccgccgg gaagatcgct gtggcactgc ctccaagatg ggcaatggcg cgggcgacgt
     6181 tgatgccgcc gcccccgggt tcgaacaccg gtgcggtaca gcgcagtttt ccttcgggat
     6241 aaatttgcgg ggtaattgtt gcgctatcga gagagggcgc aagtgtcaac gtatagatac
     6301 gtaccatcat ttcctcctat aggctgattt cagtctggca ccgaatcagc ggaaagtgaa
     6361 gaaattaaca atatgattta ttggagcttt atttaaaatt ttgcagataa atatatataa
     6421 ataaaaatct gttttcgcca catgcttatt caaaaaatga aataagaata cattgctaag
     6481 ttattcgcgc ctttttataa ttcgttacct ttccagggac gctaatcttt gatccttaag
     6541 aaagtttccg ggaccgcgat ggtctctttt tttgttgtac ggactcttga taaatgaagc
     6601 ttttgaagac agttcctgcc atcgttatgt tggcgggggg catgtttgcg tcactgaatg
     6661 cagctgccga tgattccgtt tttactgtca tggacgatcc tgccagcgct aaaaaacctt
     6721 tcgaaggtaa tctgaacgca ggatatcttg cacaatcagg caatacgaaa agctcctcac
     6781 ttacggctga caccaccatg acctggtatg gacaaaccac tgcctggtcg ctgtggggga
     6841 acgccagtaa tacttcttct aatgatgagc gttcttcaga aaaatatgcg gcgggcgggc
     6901 gtagccgttt taatttaact gattatgact atttatttgg tcaggcaagc tggctgacag
     6961 accgttataa cggctatcga gagcgcgatg tgttgaccgc gggttatggt cgccagtttt
     7021 tgaatggccc ggtacacagt ttccgctttg aattcggccc aggtgtgcgt tacgacaaat
     7081 ataccgacaa tgccagtgaa acccagccgc tgggctatgc ctccggggct tatgcatggc
     7141 agttgactga caacgctaaa tttacgcagg gtgtttcggt ctttggcgcg gaagatacaa
     7201 cgctgaactc cgaaagcgca ttaaatgttg ctattaatga acattttgga ctgaaggtgg
     7261 cttacaacgt cacatggaac tctgaacctc cagagtctgc accggaacat accgatcgtc
     7321 ggacaacctt atcgctgggc tacagcatgt aaaattatct gccggaccgt gttatgcggt
     7381 ctggctgtct tcgtttgtca ctgctcagcc ccaaatcatt gctgcccaac gtagcaacaa
     7441 catagcgcca cagattgcca gtagcaccac caccatcttc cagtgttttt tatcaaagcg
     7501 tttagtcggc ataccagtgt cctgcatatc tcttgtgcgt gataacggag tgtaacaaaa
     7561 gggataggca tcaagggatt ttttgttatt acagccgtcg ggtggcgttg tgcacatggt
     7621 cactgtttct gcgttttatg aaaaaaataa aattatgtat cgatatatat cgtaaatagc
     7681 tggttataaa tacgtcttta tttcatcagc actaaaactc aggctttggc tgatgacatt
     7741 tatagcagca taatatgttt ctacagtttt ataatgctta tgatgaatgg ctatatgaac
     7801 agctgacatg ttgtcatgat ttttagcggc caatagtgta aataactgat gttgactaaa
     7861 agaatgtttt tttgcaaaag cacccagcgt cgatatataa gataacacga cgtcttcatt
     7921 acccttgctc atggcgatgt ataatgcagg ggttccctgt gctgtagctc cttttaataa
     7981 atccatgatg ttagctttgc tcaacttgta tttaaaggcg atgccattaa ttttagagag
     8041 gaaccgtgtg acacacaaag ggtgattatt ttccattgcg gcgtataatc ctggctcacc
     8101 atcatgtatt ttcatggcta aaatactcat caccattttt ttgtttaaaa gttcttgtgc
     8161 tattggagtg gttaatgcat tcagaaagat atcgattatt tcatcgaatc catacaacat
     8221 ggagatatag agtccaggtg tttcgttagc acttttagtt tgcagtaatt taaccagatt
     8281 atcttcatga atgatatgat tgtataccag tgactgaatt tcttgcataa atatttttac
     8341 gttgtcagtt tgtccgttca tcatagccag aaataaagca ggtgtaccaa cgctattctg
     8401 cgcagttatt atttcaatga tttctttttc agggagtatg ggcaactgtt tgaatagtgt
     8461 aataagctat tgagtctggc ctgtggccat tattaacaac attgccgatg gatgtatagg
     8521 ggcaaatggc attcgtgtcg tgatacccgc taaaatagca actggaactc cctcaggtaa
     8581 tagaggaaga atagcaattg cacagttgcg aacataccta tcagcccagg tctgagcatc
     8641 gggagctgaa ctgatgaact tatcaagcga gagaaagccc cgtgctgccc tgtgggtgcc
     8701 tctgacggca acgttggtat cgttcgggtc gtaaacagac acaacataat ttaattgtcc
     8761 atacactgac tgctttattc gtagccttac tgacaaagta tgattgtcta tatataaaat
     8821 cgcggacata tgactgacat tgtttgccat cattcgacaa aagaaaatat ctaaagcgaa
     8881 gccaaaatcc ttactcgcga taacgcgact ccctgcagga ggatggcgaa gtaatgctct
     8941 gaactccgcg tcccggacta aggggacatg ctgctgtatg gattcaacgg aggagtagtg
     9001 tttaatcagg ttgactttgc cttttccatt tctcaatgaa tcaagggcgt attgcaatga
     9061 cagatggcga caaaaaatag cgtcagaagg agattgcgaa aaaaatgcac taccattgag
     9121 atttactgtt ggttgtatag ttttttctct atggattttt ttagacagtt tcatacaatg
     9181 gttacgatct tcggcagtta actgttcctg ttcaatataa tccagtatgc ttcgcaaatg
     9241 gttagcctta ataggcgtgg tttccagaaa taagggaatg atttcttttt tatagtgtaa
     9301 tgtaatgagt atattttctg catggatggg tgaagtggca aagttaatga taaaaagggc
     9361 agtggaataa caattttgtg aaatttttcg acagtttgcg gtaaattcct gagtaacaca
     9421 gtatgacagc atcgaactta agtcagaggt atcaagtagg gtgatgagtt ggcgcgcaaa
     9481 ttgattaata ggctctgatg aaggcgttgg tatgtcgcta tcgatgtgca atctatgagg
     9541 agacttataa tgggttctga taatgatatc gttttgactc ataatctatt cgccttatat
     9601 atgtctggag gttttgagga tgatgattaa ttattatcgc cagaacgaaa tgtgcacgaa
     9661 aaattgctat aagaattgtg tcacttttat cattgtgata tgaccacttc gtcattaatc
     9721 tcatgagcgt ttaaaatact accgccaaat cacgcaacga actttgctgg attcagttta
     9781 tgctgctgta aatccgctcg cagtaaacct ttcagacgca ccgtgatgtt atcagtggtt
     9841 ctttaagcgt tttgctggtg tactcactac aaacgaattg cgaatcgaat caatgtgaaa
     9901 cggaaaggta caatctccct ttttttagtg ttaacatcgt tcaaccgggt ggaaggtgag
     9961 aggttaatgt ctttcatttg tgtacctgat tgcgaaccaa tttagcattt gttggctaga
    10021 tggtttcgca atgaactgtt aataaacaaa tttttctttg tatgtgatct ttcgtgtggg
    10081 tcaccactgc aaattaagga tataaaatgc ctgttataac tcttcctgat ggcagccaac
    10141 gccattacga ccacgctgta agccccatgg atgttgcgct ggacattggt ccaggtctgg
    10201 cgaaagcctg tatcgcaggg cgcgttaatg gcgaactggt tgatgcttgc gatctgattg
    10261 aaaacgacgc acaactgtcg atcattaccg ccaaagacga agaaggtctg gagatcattc
    10321 gtcactcctg tgcgcacctg ttagggcatg cgattaaaca actttggccg cataccaaaa
    10381 tggcaatcgg cccggttatt gacaacggtt tttattacga cgttgatctt gaccgcacgt
    10441 taacccagga agatgtcgaa gcactcgaga agcggatgca tgagcttgct gagaaaaact
    10501 acgacgtcat taagaagaaa gtcagctggc acgaagcgcg tgaaactttc gccaaccgtg
    10561 gggagagcta caaagtctcc attcttgacg aaaacatcgc ccatgatgac aagccaggtc
    10621 tgtacttcca tgaagaatat gtcgatatgt gccgcggtcc gcacgtaccg aacatgcgtt
    10681 tctgccatca tttcaaacta atgaaaacgg caggggctta ctggcgtggc gacagcaaca
    10741 acaaaatgtt gcaacgtatt tacggtacgg cgtgggcaga caaaaaagca cttaacgctt
    10801 acctgcagcg cctggaagaa gccgcgaaac gcgaccaccg taaaatcggt aaacagctcg
    10861 acctgtacca tatgcaggaa gaagcgccgg gtatggtatt ctggcacaac gacggctgga
    10921 ccatcttccg tgaactggaa gtgtttgttc gttctaaact gaaagagtac cagtatcagg
    10981 aagttaaagg tccgttcatg atggaccgtg tcctgtggga aaaaaccggt cactgggaca
    11041 actacaaaga tgcaatgttc accacatctt ctgagaaccg tgaatactgc attaagccga
    11101 tgaactgccc gggtcacgta caaattttca accaggggct gaagtcttat cgcgatctgc
    11161 cgctgcgtat ggccgagttt ggtagctgcc accgtaacga gccgtcaggt tcgctgcatg
    11221 gcctgatgcg cgtgcgtgga tttacccagg atgacgcgca tatcttctgt actgaagaac
    11281 aaattcgcga tgaagttaac ggatgtatcc gtttagtcta tgatatgtac agcacttttg
    11341 gcttcgagaa gatcgtcgtc aaactctcca ctcgtcctga aaaacgtatt ggcagcgacg
    11401 aaatgtggga tcgtgctgag gcggacctgg cggttgcgct ggaagaaaac aacatcccgt
    11461 ttgaatatca actgggtgaa ggcgctttct acggtccgaa aattgaattt accctgtatg
    11521 actgcctcga tcgtgcatgg cagtgcggta cagtacagct ggacttctcc ttgccgtctc
    11581 gtctgagcgc ctcctatgtg ggcgaagaca acgagcgtaa ggtaccggta atgattcacc
    11641 gcgcaattct ggggtcgatg gaacgtttca tcggtatcct gaccgaagaa ttcgctggtt
    11701 tcttcccgac ctggcttgcg ccagttcagg ttgttatcat gaatattacc gattcacagt
    11761 ctgaatacgt taacgaattg acgcaaaaac tatcaaatgc gggcattcgt gttaaagcag
    11821 acttgagaaa tgagaagatt ggctttaaaa tccgcgagca cactttgcgt cgcgtcccat
    11881 atatgctggt ctgtggtgat aaagaggtgg aatcaggcaa agttgccgtt cgcacccgcc
    11941 gtggtaaaga cctgggaagc atggacgtaa atgaagtgat cgagaagctg caacaagaga
    12001 ttcgcagccg cagtcttaaa caattggagg aataaggtat taaaggcgga aaacgagttc
    12061 aaacggcgcg ccctaaccgt atcaatggcg aaattcgcgc ccaggaagtt cgcttaacag
    12121 gtctggaagg cgagcagctt ggtattgtga gtctgagaga agctctggag aaagcagaag
    12181 aagccggagt agacttagtc gagatcagcc ctaacgccga gccgccggtt tgtcgtataa
    12241 tggattacgg caaattcctc tatgaaaaga gcaagtcttc taaggaacag aagaaaaagc
    12301 aaaaagttat ccaggttaag gaaattaaat tccgtcctgg tacagatgaa ggcgactatc
    12361 aggtaaaact ccgcagcctg attcgctttc tcgaagaggg tgataaagcc aaaatcacgc
    12421 tgcgtttccg cggtcgtgag atggcgcacc agcaaatcgg tatggaagtg cttaatcgcg
    12481 tgaaagacga tttgcaagaa ctggcagtgg tcgaatcctt cccaacgaag atcgaaggcc
    12541 gccagatgat catggtgctc gctcctaaga agaaacagta aggccttcaa gtagcactgt
    12601 ctgtggagcc ttcgggcttc acaggctttg ttcgcctttg tttcgtttat taacaatgcg
    12661 aagtggaagt tattaaaatg ccaaaaatta agaccgtacg cggtgctgct aagcgcttca
    12721 aaaaaaccgg taaaggtggt tttaagcaca agcacgctaa cctgcgtcac attctgacca
    12781 aaaaagcgac caaacgtaaa cgtcacctgc gtccgaaagc catggtttcc aaaggcgatc
    12841 tgggcctggt aatcgcgtgc ctgccgtacg cataagccgt taacgttttt aactttttaa
    12901 ttagaatata gatacaggag agcacatatg gctcgcgtaa aacgtggtgt tattgcacgc
    12961 gcacgtcaca agaaaatttt gaaacaagct aaaggctact acggtgcgcg ttctcgcgta
    13021 taccgcgttg ccttccaggc tgttatcaaa gctggtcagt atgcttaccg tgaccgtcgt
    13081 caacgtaagc gtcagttccg tcaactgtgg attgcgcgta tcaacgcagc agcacgtcag
    13141 aacggtattt cttacagcaa attcatcaat ggcctgaaaa aagcctctgt tgaaatcgac
    13201 cgtaagatcc tggctgatat cgcagtattc gacaaagtag cgttcaccgc tctggttgaa
    13261 aaagcgaaag cagctctggc ataagccagt tgaaagaggg agctagtctc cctcttttcg
    13321 tttcaacgcc atcaaaacat tgacttttat cgccgtagcc ttttcaataa aggtcttttg
    13381 aagagtacca aaaggtaacg caagcaatga atgctgctat tttccgcttc tttttttact
    13441 ttagcacctg aatccaggag gctagcgcgt gagaagagaa acggaaaaca gcgcctgaaa
    13501 gcctcccagt ggaggctttt tttgtatgcg cgttttgaaa aataaaaagc cagtctatca
    13561 ggcgtaattg tcgcagccag tctggacacg gccagcgcgg agaaaccgga gcgtatttaa
    13621 gtacgtgaga atttcgagca ctgcccggga ccaaaatggc aagtaaaata gcctgatggg
    13681 ataggctcta agtccaacga accagtgtca ccactgacac aatgaggaaa accatgtcac
    13741 atctcgcaga actggttgcc agtgcgaagg cggccattag ccaggcgtca gatgttgccg
    13801 cgttagataa tgtgcgcgtc gaatatttgg gtaaaaaagg gcacttaacc cttcagatga
    13861 cgaccctgcg tgagctgccg ccagaagagc gtccggcagc tggtgcggtt atcaacgaag
    13921 cgaaagagca ggttcagcag gcgctgaatg cgcgtaaagc ggaactggaa agcgcagcgc
    13981 tgaatgcgcg tctggcggcg gaaacgattg atgtctccct gctaggtcgt cgcattgaaa
    14041 acggcggtct gcatccggtt acccgtacca tcgaccgtat cgaaagtttc ttcggtgagc
    14101 ttggctttac cgtggcaacc gggccggaaa tcgaagacga ttatcataac ttcgatgctc
    14161 tgaacattcc tggtcaccac ccggcgcgcg ctgaccacga cactttctgg tttgacacta
    14221 cccgcctgct gcgtacccag acctctggcg tacagatccg caccatgaaa gcccagcagc
    14281 caccgattcg tatcatcgcg cctggccgcg tttatcgtaa cgactacgac cagactcaca
    14341 cgccgatgtt ccatcagatg gaaggtctga ttgttgatac caacatcagc tttaccaacc
    14401 tgaaaggcac tctgcacgat ttcctgcgta acttctttga ggaagatttg cagattcgct
    14461 tccgtccttc ctacttcccg ttcaccgagc cttctgcaga agttgacgtc atgggtaaaa
    14521 acggtaaatg gctggaagtg ctgggttgcg ggatggtgca tccgaacgtg ctgcgtaacg
    14581 ttggcatcga cccggaagtt tactctggtt tcgccttcgg gatggggatg gagcgtctga
    14641 ctatgttgcg ttacggcgtc accgacctgc gttcattctt cgaaaacgat ctgcgtttcc
    14701 tcaaacagtt taaataaggc aggaatagat tatgaaattc agtgaactgt ggttacgcga
    14761 atgggtgaac ccggcgattg atagcgatgc gctggcgaat caaatcacta tggcgggcct
    14821 ggaagttgac ggtgtagaac cggttgccgg tagcttccac ggcgtggtcg ttggtgaagt
    14881 ggttgagtgt gcgcagcatc cgaacgctga caaactgcgt gtgacaaaag tgaatgtcgg
    14941 cggcgatcgc ctgctggaca tcgtctgtgg tgcgccaaac tgccgtcagg gcctgcgtgt
    15001 ggcggtagct accattggtg ctgttctgcc gggtgatttc aaaattaaag cggcgaaact
    15061 gcgtggcgaa ccgtctgaag ggatgctgtg ctccttctct gaactgggaa tttctgacga
    15121 tcacagcggc attatcgaac tgcctgcaga tgcgccgatt ggcactgaca tccgcgaata
    15181 cctgaaactc gatgacaaca ccatcgaaat cagcgtaacg ccaaaccgtg ccgactgctt
    15241 aggtatcatt ggtgttgcgc gtgacgttgc cgtgctgaat cagctgccgc tggttgaacc
    15301 ggaaatcgtt ccggttggtg cgaccatcga cgacacgttg ccgattacag tcgaagcgcc
    15361 tgaagcctgc ccgcgctatc ttggccgtgt ggtaaaaggc attaacgtta aagcgccaac
    15421 tccgctgtgg atgaaagaaa aactgcgtcg ttgcgggatc cgttctatcg atgcagttgt
    15481 tgacgtcacc aactatgtac tgctcgaact gggccagccg atgcacgctt tcgataaaga
    15541 tcgcattgaa ggcggcattg tggtgcggat ggcaaaagag ggcgaaacgc tggtgctgct
    15601 tgatggcact gaagcgaagc tgaatgctga cactctggtc atcgccgacc acaacaaggc
    15661 gctggcgatg ggcggcatct ttggtggcga acactctggc gtgaatgacg aaacgcaaaa
    15721 cgtcttgctg gaatgcgctt tcttcagccc gctgtctatc accggtcgtg ctcgtcgtca
    15781 tggcctgcat actgatgcgt ctcaccgtta tgagcgtggc gttgatccgg cactgcagca
    15841 caaagcgatg gaacgtgcga cccgtctgct gatcgacatc tgcggtggtg aggctggtcc
    15901 ggtaattgat atcaccaacg aagcaacgct gccgaagcgt gcaaccatca ctttacgtcg
    15961 tagcaaactg gatcgcctga tcggccatca tattgctgat gaacaggtaa ctgacattct
    16021 gcgtcgtctc ggctgcgaag tgaccgaagg caaagacgag tggcaggcag ttgcgccgag
    16081 ctggcgtttc gatatggaga ttgaagaaga tctggttgaa gaagtcgcgc gtgtttacgg
    16141 ctacaacaac atcccggatg agccggtaca ggcaagcctg attatgggta ctcaccgtga
    16201 agctgacctg tcgctcaagc gcgtgaaaac gctgctcaac gacaaaggct atcaggaagt
    16261 gatcacctac agcttcgttg atccgaaagt gcagcagatg atccatccag gcgttgaagc
    16321 cttactgctg ccaagcccga tctctgttga aatgtcagca atgcgtcttt ctctgtggac
    16381 cggcctgctg gcaaccgtgg tgtacaacca gaaccgtcag cagaaccgtg tgcgcatttt
    16441 cgaaagcggt ctgcgtttcg taccagatac tcaggcaccg ttgggcattc gtcaggatct
    16501 gatgttagcc ggtgtgattt gcggtaaccg ttacgaagag cactggaacc tggcaaaaga
    16561 gaccgttgat ttctatgatt tgaaaggcga tcttgaatcc gttctcgacc tgaccggtaa
    16621 actgaatgag gttgagttcc gtgcagaagc gaatccggca ctgcatccgg ggcaatccgc
    16681 agcgatttat ctgaaagggg aacgtattgg ttttgttgga gttgttcatc ctgaactgga
    16741 acgtaaactg gatcttaacg gtcgcactct ggtgttcgaa ctggagtgga acaagctcgc
    16801 agaccgcgtg gtgcctcagg cgcgcgagat ttctcgcttc ccggcgaacc gtcgtgacat
    16861 cgcggtggtg gtcgcagaaa acgttcccgc agcggatatt ttatccgaat gtaagaaagt
    16921 tggcgtaaat caggtagttg gcgtaaactt atttgacgtg taccgcggta agggtgttgc
    16981 ggaggggtat aagagcctcg ccataagcct gatcctgcaa gataccagcc gtacactcga
    17041 agaagaggag attgccgcta ccgtcgccaa atgtgtagag gcattaaaag agcgattcca
    17101 ggcatcattg agggattgaa cctatggcgc ttacaaaagc tgaaatgtca gaatatctgt
    17161 ttgataagct tgggcttagc aagcgggatg ccaaagaact ggttgaactg tttttcgaag
    17221 agatccgtcg cgctctggaa aacggcgaac aggtgaaact ctctggtttt ggtaacttcg
    17281 atctgcgtga taagaatcaa cgcccgggac gtaacccgaa aacgggcgag gatattccca
    17341 ttacagcacg gcgcgtggtg accttcagac ccgggcagaa gttaaaaagc cgggtcgaaa
    17401 acgcttcgcc caaagacgag taatctgatc taactaaaaa ggccgctctg cggccttttt
    17461 tcttttcact gtcgaagagt caccgtaaaa tcaacgccat gacacttcag cagaacggat
    17521 accatgctga cacttgcccg ccaacaacag cgacaaaata ttcgctggtt attatgcctg
    17581 tcagttttga tgctgctggc gcttctctta agcctttgcg ccggtgaaca atggatttcg
    17641 ccaggtgact ggtttactcc tcgtggcgaa ctgttcgtct ggcaaattcg cctgccacgt
    17701 acgctggctg tattgctggt tggtgcggcg ctggctatat ccggcgctgt aatgcaggcg
    17761 ttgtttgaaa atcctctggc agaacctgga ctacttggcg tctctaacgg cgcaggcgtg
    17821 gggcttatcg ccgcggtatt gcttgggcaa gggcaactcc ccaactgggc gctagggctg
    17881 tgtgcgattg ctggcgcgct tatcatcact ttaatactct tacgtttcgc ccgtcgtcat
    17941 ctttcgacca gtcggttatt gctggctggc gttgcattag ggattatctg tagcgcacta
    18001 atgacgtggg ctatctactt ttccacctct gttgatttac gtcagctgat gtactggatg
    18061 atgggcggtt ttggcggcgt agactggcgg caaagctggc tgatgctggc attgatcccc
    18121 gtgttgttgt ggatctgttg tcagtccagg ccgatgaata tgttagcact tggcgagatc
    18181 tcggcgcggc aactgggttt acccctgtgg ttctggcgca atgtgctggt ggcagcgacc
    18241 ggctggatgg ttggcgtcag tgtggcgctg gcgggtgcta tcggctttat tggtctggtg
    18301 atcccacata ttcttcggtt gtgtggttta accgatcatc gcgtattact tcccggctgc
    18361 gcgctggcag gggcgagcgc agtgctgctg gccgatattg tagcgcgcct ggcattagct
    18421 gccgcagagc tgcctattgg cgtggtcacc gcaacgttgg gtgcgccggt gtttatctgg
    18481 ttattgttaa aagcaggacg ttagccgcaa aaagacggtc tatgattaaa agctaagatt
    18541 tttactgacc acacccagga gaaacgatgc aagattccat tctgacgacc gtagtgaaag
    18601 atatcgacgg tgaagtgacc acgctggaga agtacgccgg taatgtgctg ttgattgtca
    18661 atgtcgcctc aaagtgtggc ttaacgccgc aatatgagca gttggagaat attcagaaag
    18721 cctgggccga tcgaggtttt gtggtgctgg gattcccgtg caaccagttt ctggaacaag
    18781 aaccgggcag cgatgaagag attaaaactt actgtaccac cacatggggg gtgacgttcc
    18841 cgatgttcag taagattgaa gttaacggcg aaggacgcca tccgctgtat caaaaattga
    18901 ttgccgcagc gccgaccgca gtcgcgccgg aagagagcgg attctatgcc cgtatggtca
    18961 gcaaaggtcg tgcaccgctg taccctgatg atattttatg gaattttgag aaatttttag
    19021 ttggcaggga cggattggtc atccagcgtt tttccccgga tatgacgccg gaagacccca
    19081 ttgtgatgga aagcattaaa ctggcgttgg caaaataatg tctattgtga tgcagttaca
    19141 ggatgttgcg gaatctaccc gcctggggcc actttctggc gaggttcggg ctggggagat
    19201 cctgcacctg gtggggccga atggcgcggg taagagtacc ttactggcac gaatggccgg
    19261 aatgaccagc ggtaagggaa gtattcagtt cgcggggcaa ccactggaag catggtccgc
    19321 aacaaaactc gcgctgcatc gcgcctatct ttcacaacag cagacgccgc cgtttgcaat
    19381 gccggtctgg cactatctga cactgcatca gcacgataaa acgcgtaccg aactactgaa
    19441 tgatgtcgca ggggcgctgg ctcttgatga caaactcgga cgtagcacca atcaactttc
    19501 cggcggtgaa tggcaacgcg tacgtcttgc tgcggtggtg ctgcaaatca caccacaagc
    19561 caatcccgca ggccaattgc tgcttcttga tgagccgatg aacagtcttg atgttgcgca
    19621 acaaagtgcg ttagacaaaa ttctgagcgc tctgtgtcag caaggactgg cgattgtgat
    19681 gagcagtcac gatctcaacc acacattgcg tcatgcgcat cgggcgtggt tgctaaaagg
    19741 tggaaaaatg ctggccagtg gacgcagaga agaggtgctc acgccgccaa atctggcgca
    19801 ggcctatggg atgaattttc gccgtctgga tatcgaaggt cacagaatgc tgatttcgac
    19861 catctgattt cattagtatc ttgaaaatcg ggtaattacg acgctaaatt aatgcaagaa
    19921 taaaaacaga ggaaccgtac ggaatgcgtt tctgccttat tttgatcaca gcactgtttc
    19981 tggccgggtg tagccaccat aaagcaccgc cgccaaatgc cagactttct gattcgatta
    20041 ccgttattgc cggtttgaac gaccagctac aaagctggca tggcacgccg tatcgttatg
    20101 gtggcatgac gcggcgcggt gtggactgtt cgggatttgt ggttgtaacg atgcgcgatc
    20161 gtttcgattt gcagctgccc cgagaaacca aagaacaagc ctctatcggc acgcaaattg
    20221 ataaagacga gttgctgcct ggtgacctgg tctttttcaa aacgggttcc ggacaaaatg
    20281 gtttgcatgt aggtatttac gataccaaca accaatttat ccacgcctct accagcaagg
    20341 gagtgatgcg ttcctcactt gataatgtct attggcagaa aaatttctgg caggcgagac
    20401 gaa
//
LOCUS       DAZPDU010000067        20347 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZPDU010000067 DAZPDU010000000
VERSION     DAZPDU010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20347
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.068"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            249..749
                     /gene="tssB"
                     /locus_tag="ACHURS_002213"
     CDS             249..749
                     /gene="tssB"
                     /locus_tag="ACHURS_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFR1237935.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GLVSNK"
     gene            784..1008
                     /locus_tag="ACHURS_002214"
     CDS             784..1008
                     /locus_tag="ACHURS_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1237936.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            1059..2534
                     /gene="tssC"
                     /locus_tag="ACHURS_002215"
                     /pseudo
     CDS             1059..2534
                     /gene="tssC"
                     /locus_tag="ACHURS_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
     gene            2541..2954
                     /gene="tssE"
                     /locus_tag="ACHURS_002216"
     CDS             2541..2954
                     /gene="tssE"
                     /locus_tag="ACHURS_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFR1237937.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            2958..4808
                     /gene="tssF"
                     /locus_tag="ACHURS_002217"
     CDS             2958..4808
                     /gene="tssF"
                     /locus_tag="ACHURS_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFR1237938.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQIEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWPLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            4772..5854
                     /gene="tssG"
                     /locus_tag="ACHURS_002218"
     CDS             4772..5854
                     /gene="tssG"
                     /locus_tag="ACHURS_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFR1237939.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLAHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5879..7159
                     /gene="tagH"
                     /locus_tag="ACHURS_002219"
     CDS             5879..7159
                     /gene="tagH"
                     /locus_tag="ACHURS_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HFR1237940.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSAAEMAQRHVAVTPLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7156..7680
                     /gene="tssJ"
                     /locus_tag="ACHURS_002220"
     CDS             7156..7680
                     /gene="tssJ"
                     /locus_tag="ACHURS_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFR1237941.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGIGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7683..9014
                     /gene="tssK"
                     /locus_tag="ACHURS_002221"
     CDS             7683..9014
                     /gene="tssK"
                     /locus_tag="ACHURS_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFR1237942.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQNFPDERSRNIY
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDARTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHHARRSQLHPEDFYLDLAGLLGELMTF
                     TEPSRLPCPLDVYDHHDLTKIFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADIH
                     DTELLQSATFVLAVAANMPVDQIQRQFIQQSKISSPEKIRNMVSVQIPCIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            9019..9780
                     /gene="icmH"
                     /locus_tag="ACHURS_002222"
     CDS             9019..9780
                     /gene="icmH"
                     /locus_tag="ACHURS_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFR1237943.1"
                     /translation="MDEGSLSLPPFTGHDEKSQRNYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWICFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9789..12572
                     /gene="tssH"
                     /locus_tag="ACHURS_002223"
                     /gene_synonym="clpV"
     CDS             9789..12572
                     /gene="tssH"
                     /locus_tag="ACHURS_002223"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFR1237944.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTMTDASDTLLARYAKNMTADARNGRLDPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQQLLGVAEDD
                     AAPLPDADTVEDTQPESESESESESEQDNTGAEPADEADREQPEETAETVSPVQRLAQ
                     LTAELDALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLTKWLGDTI
                     KGQDLAIASLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQY
                     LTTINMSEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDV
                     LNLFYQAFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEVLYPVLA
                     DFFKPALLARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGVEVVIEPEVTDEIM
                     SRVTRAENGARMLESVIDGDMLPPLSLLLLQKMAANTAIARIRLSAVDGAFTADVEDA
                     QNDESVTKDETVL"
     gene            12569..13312
                     /gene="vasI"
                     /locus_tag="ACHURS_002224"
     CDS             12569..13312
                     /gene="vasI"
                     /locus_tag="ACHURS_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFR1237945.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            13317..14729
                     /gene="tssA"
                     /locus_tag="ACHURS_002225"
     CDS             13317..14729
                     /gene="tssA"
                     /locus_tag="ACHURS_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFR1237946.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPNWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWAVAAPQNMAHKKRFASQVIKRFETGIEVFSQNAATAQRDALSGQLAKLAQCWQSHN
                     APELAQATDDLFALYQRAFNRAAPAPVPTPAASGSSPQTTATSESGVTQPSAPAPQIV
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQNITSTPQAESDGRTPLAA
                     VSADMVADYHAQLGSADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADAIR
                     DEVIRFLERLPQLTGLLFNDHTPFISEQTKQWLAASPDAKVAPVAQIGEESKAARACF
                     AEQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLVQLAQQQYRMLFRMGLQMM
                     VADWEPSLLEQLEQKFTAEQ"
     gene            14838..18272
                     /gene="tssM"
                     /locus_tag="ACHURS_002226"
                     /pseudo
     CDS             14838..18272
                     /gene="tssM"
                     /locus_tag="ACHURS_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
     gene            18283..19732
                     /locus_tag="ACHURS_002227"
                     /pseudo
     CDS             18283..19732
                     /locus_tag="ACHURS_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            19659..20136
                     /locus_tag="ACHURS_002228"
                     /pseudo
     CDS             19659..20136
                     /locus_tag="ACHURS_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
BASE COUNT         4811 a         5563 c         5617 g         4356 t
ORIGIN      
        1 aattattgcc ataatacacc tgacgcgaat tgcacaggtt cacacaatct atttaacgct
       61 tgcagaatac ccctctttac gatcgataaa aagtcctttt cttatttatt ttgaagcgta
      121 aaatgcgggg tgatgaagga gtggtgattt tattttcacc gccattaggg cggcattgcc
      181 gatatttttt tgcggtacaa atatgccgtg ttcatggcct gtacgagata acatgacgga
      241 tcacttttat gagcaaaaaa tttgaaggca gcgtcgcgcc ccgcgagcgt attaatatat
      301 cgtatgtccc aaaaacagat ggccagacgg ctgaagtgga acttccgctt aacatgctcg
      361 ttgtcggcga taccggaaat acgcaggaaa catccccact ggatgaacgt caggcagttt
      421 ccgttaacaa gcacaatttc ggtgcagtga tggctgaagc agccattggc ctcaatttta
      481 ctgtccccgc cacgctgaag ggaagcacaa cagacgatga actgaatgtg gcgctgaata
      541 tcaaatcact ggatgatttt tcaccagata gcgtggcccg gcaggttccg gaagtgaata
      601 aattgctgga actgcgtgaa gcactgactg cactgaaggg gccgatgggt aaccttccgg
      661 cattccgtac ccagctccag gcattgctgg aaaatgaaga gtcccgcgag cagttgctca
      721 aagaaatcgg tctggtgagc aataaataac caccactatt tcattaaaca gggttatcca
      781 ggcatgacgg cacacaaaaa tatctccggg acataccacc tctctgttgc agacattctg
      841 caggtggttt accaggtatg tttcagcccg tccgttgaaa tcaaccagga tggtgtggct
      901 gcgctgatta caacactgga tcgccgtatc agtgatctat tggatgagat tatccatttt
      961 tgtgagtttc agcagagcgc gtcgcactgg cagcgcgtac ttcactgatg gcgaaaagcc
     1021 ggtttcagaa acacagctaa aacagaagga ttttcagtat gtctttacag gaagaggaac
     1081 tcgtttccag tcatgccggg cagcctgagc aggcatcgtc gctgcttgat caaatcatgg
     1141 cacagacccg tattcagccc ggttcggaag gttatgacgt tgccctccag ggtgtgacag
     1201 cctttattgc cagcatcctg caatccactg catctgccga accggtcaat aaactggccg
     1261 tggacagcat gattgcagat attgatgaac gcatcagccg ccagatggat gtcatcatcc
     1321 atgcgcctgc ctttcagcag gtcgagtcgt tctggcgctc actgaaaacc atggtggatc
     1381 gcgttgattt ccgtgaaaac atcaaggtca acgtgcttca tgtcaccaag caggaacttc
     1441 tggaagactt tgagtttgcg ccagaaatta ttcagtccgg attttataag cacgtttatt
     1501 cgtccggttt cggacagttt ggcggtaaac ccatcgctgc cgtgctgggc gcttatgagt
     1561 tcaaaaatac cgcgcctgac atgaaactgc tgcaatacgt cagtgccgtg ggcgcaatgg
     1621 cgcacgcacc gttcctgtcc tccgtttccc cggagtttat ggggctgaac tcgtggaccg
     1681 aactgcctaa tatcaaagat ctttatgcca tctttgaagg tccggcctac accaaatggc
     1741 gcgccctgcg tgactcggaa gattcgcgct atttagggct tacggcaccc cgcttcctgc
     1801 ttcgccagcc ctattcacca acagataacc ctgttaagaa ctttaactat tacgaagatg
     1861 tcagccagaa ccacgaagat tatctgtggg gtaatacggc ctggatgctg gcatgcaata
     1921 tcgcagacag ttttgccaaa taccgctgat gtccaaacat tatcggtccg caaagcggcg
     1981 gcgcagtgaa agatctgccg atgcatctgt tcgaaacgat ggggcagatt caggccaaaa
     2041 tcccaaccga ggtgctggtc accgatcgcc gcgaatttga actggctgaa gagggtttta
     2101 tcacccttac catgcgtaaa gactctgata atgcagcctt tttctctgca aactcggtac
     2161 aaaaaccgaa gcactttccg ggaaaagatg cagaaaccaa ctataagctg ggtacgcagc
     2221 ttccttatct cttcatcatc aatcggttag cgcactacat caaggtgttg cagcgtgaac
     2281 aactggggtc atggaaagaa aggagcgact tagaaagaga gcttaatacc tggatccgtc
     2341 agtatgttgc cgaccaggaa aacccgcctg cagacgtgcg cagccgcaaa cctctgcgcg
     2401 ctgccaaagt tgaggttatg gatgtagaag gcgaacccgg ctggtaccag gttgcgctaa
     2461 gcgtgagacc tcattttaaa tttatggggg caaattttga gctttccctg gttggccggt
     2521 tagacaggga gtaacacacc atgataccgg atcgggaaag aaccacaggt tctctttttg
     2581 agcgtatgga agcctcttcg gccagaaacc ggcagggagg cagtattcat tcgctcaggc
     2641 aatccattcg ccagaaccta cgaaacattc ttaatacccg gtccggtagt tgccggggtg
     2701 cgcctgagct gggcattgat gaaccggaag gggctgaaaa cttccgcgaa tccatgagtc
     2761 gggccatcga gcaatgtata gaacgctatg aaccccggat ctcacacgct gaagtgcagg
     2821 cagtggtttc atccgcctcc agcccgctgg atatgacgtt tcatattaca gcctgggtga
     2881 catttaacga aacgcatgag gtgcttgaat ttgatatggc accgaatggc agccagcatt
     2941 accgggtgga ctgacaaatg gaatttgaag aacgctattt ccgggaagaa ctcgattacc
     3001 tgcgccagct tagcaagctg ctggcaacgg aaaaacccca tctggcccgc ttcctggccg
     3061 aaaaagatgc agatccggat attgaacgcc tgctggaagg ggtggctttt cttaccggca
     3121 atctccgcca gaaaattgag gatgaattcc cagaactgac gcacgggctt attaagatgc
     3181 tatggcctaa ttacctgcgt ccggttccgg caatgaccct tattgaatat acgccggata
     3241 tggataagtc ttctgtaccg gtgttaatcc cccgtaatga gcagtttaca accaacgccg
     3301 gggaaatcag agttgatgaa gtgctgccct ctgatgctaa aaaggaggag ccgcctccct
     3361 gtaccttcac cctctgccgg gatatctggc tgctgcccgt tcgcctggag cagattgaaa
     3421 accgcagtac gacccgtaat ggtgttatca acatcacctt ttcggtcgca ccgggaacgg
     3481 acttccgcac gctggatctg aacaaacttc gcttctggct cggcaatgac gacaactata
     3541 cccgtgacca gctttattta tggttctgcg aatacttgca gggtgccgac ctgactgtgg
     3601 gtgaacagca tattcgcctg cctgagttta tgctaaaagc tgtcggtttt gagccgcagg
     3661 atgccatgct gccctggccg aaaaacgtcc acagcggcta ccggatcctt caggagtatt
     3721 tctgttaccc cgatgcgttt ctcttttttg atctttgtgg ttgtccggct ttgcctgacg
     3781 gattgcaggc ggaattcttt accctgcaac tgcgtttttc gcgccctttg cccgtggaca
     3841 tccggctgcg ccgcgattcc ctgcgcctgt attgcgcacc tgccattaat ttatttatcc
     3901 accatgcaga agccatcacg ctggacaacc ggcgggcaga ctatccgctg gttcccagcc
     3961 gccattaccc acaacattac gatgtatttt ccgttaacag tgtggtgagc caggtccagg
     4021 atatgttcag gaaaaaagat ctggggcgtc ctgtttcgac gcaggccgcg cgccagtggc
     4081 cagcctttga aagtttcagc catcagatag aatacagccg gaagcgggaa gtggtgtact
     4141 ggcatcaccg gaccaaaaca tccctgttcc atcgcggctt tgatcatacc cttgccttta
     4201 tacatgctga tggcagttat ccgtcagacg aatctctgct cagtaatgaa gtggtttcgg
     4261 tatcgctgac ctgtaccaac cgtgagcttc cgtcacaaat tcgttccggc gatatcaccg
     4321 gcacaaccgg aaaaaatgcg gctgttgctt catttcgcaa cattacccgc ccgacgcaac
     4381 cactctggcc ggtcattgat ggcagcctgc actggcccct actctccgcc atgaacctga
     4441 attatctgtc attactggat acggacgcgc tgaagcaggt catcgccaac tttgatcgcc
     4501 acgcaatcca tcatccgcag acggcacggc tgtcacaaca aaagctggat gccattgagc
     4561 gtctggagac ccgccccgtt gatcgcctgt ttacgggtat tcccgtccgg gggctggctt
     4621 ccacgctgta tctgcacccg gagccgtttg tctgtgaagg ggaaatgtat ctgctcggta
     4681 cggtgctttc gcattttctg tcgctgtacg ccagcgttaa ctcattccac atgctgaccg
     4741 ttgtgaacac agaaagccag gagacatgga aatggacgga aagaatcggg cagcatcctc
     4801 ttatctgagt ccgggaaatc ccccggcaga taaagagcag aatgatccgc tggcacaggt
     4861 tttccataat gcctgctctt acaatttttt tgcgatggcg gagctgttgc accgcctggc
     4921 aaagggtgaa aaggggacgc cagaattatc cctgcgcgac gatccggcac aggaaaccct
     4981 gcgtttcagc gccgatgcca gccttgcctt cccgtgcagt gatatcagtg cgctgaaacg
     5041 ggatacgtca ggggcattca ggatgaccac cacctttatg gggttacagg ggagccagtc
     5101 gcctctcccc ggttattacc tcgctcacct ggcctggaaa gcggtacatg aacaaagtcc
     5161 ggtaggtgat tttctggata tgttcagcca ccgcctgacc cagtttgtct ggcatatctg
     5221 gcgtaagtac cgctatcaca tcagttttcg taacggcggt gtggatgcgt tctcgcagcg
     5281 catgtactcc cttgtcgggc tgggccaccg ccagcttcgc gataagctgg cgattaatca
     5341 cagcaaaatg ctggcgtatt ccgggatcct ggcaaatccg ggacgatccc cggaaattat
     5401 ctgtggcctg gtgtcccact gctttgattt gtcagaagtg acactgcaaa actggcaacg
     5461 tcgcaaggtg gatattgaac cggatcagca aaatagtctg gggagctatt ctcttaaaaa
     5521 tggcgaaaaa ctggcgggaa gatcggtact gggcaacttt gttctgggta cacgagtgcc
     5581 cgatctcagc ggaaaattcc agctcagtat taccagcctg acccgaaaac agttcctctc
     5641 ttttctgccg tccggcgaaa acttcctgcc actgacgatg ttcgtgtcct tcatcctgcg
     5701 cgatcagctt gcctgggact tacatctggg gctggcgccg gagcaggtgg gcgcaatgcg
     5761 cctgggcgat aacaaaagtg cgctgctggg ctggaccagc ttcctcggca caccggaaga
     5821 acgaccctca gtcaccatca gggtacggtc gtaaattatg ataatcacgg agcagaatat
     5881 gccggaagag aaactacaga cgctttcatt gcaggtcatt aacggcagtg agctggaaag
     5941 cgggcgggcg gcgcgctgtc tgttcacaca gcagggaaat gtcggccatg gccccgaatg
     6001 ccactggtcg gtacaggatc gtcagcagag cattccggcc caggctttta ccgttatcct
     6061 gcacgatggc acattttgtc tgcgcccgca gacggcacaa ctgtggctga atcaggcaaa
     6121 agtcacagca acatcagacc taatacagtt gcgccagggc gatgagatcc agatcggacg
     6181 gctgatggtg agggttcatc tgaaccgggg agatattccc cattacgatg aggaaatggc
     6241 cactcccgaa accatcgtta ccaatcgcga tatgctcacg gataccctgc tatcaacgga
     6301 gggtgcgcca cactatccgg gaatgactca ccggcaccag cttgcagaca ccgtggtaaa
     6361 cggtttttct gccgatccac tccaggctct tcagtccgaa agcctgatta ccacgggcga
     6421 tccgctttca ggcattgcgg ctgtccggcc atcggcaccg ctgtccgatc cggcaagtaa
     6481 tggggggatc aatactccgt ttatggatct gccgcccatt tatgccagcc ccggcgatcg
     6541 taatgacgac gtctctgcgg cagaaatggc gcaacgccac gttgcggtca cccccttact
     6601 gcgcggtctt ggcggctcgc ttaccatgag caattccgac gatgcggatg attttctgga
     6661 ggaggccgga cgaacgttac aggccgcaat aaaaggtctg ctcgatttgc agcagcagcg
     6721 taacagcctc tcagacaaac atttgcgccc gctggaagat aacccgctgc gcctgaacat
     6781 ggattacgcc accgcgctcg acgtaatgtt tgccgaaggt aaaagcccgg tacatctggc
     6841 ggctcccgcc gccgtcagtg aaagcctgcg caatgtccgc caccacgaag aagctaaccg
     6901 ggcagcgatt gtggagtcgc ttcgtgtcct gctggatgct ttctcaccac aaaatctgct
     6961 gcgccgcttt gtgcagtacc gccgcagcca tgaactgcgc cagccgctgg atgatgccgg
     7021 agcatggcaa atgtacagcc attattacga agaactggcc tccgatcgcc agcaggggtt
     7081 tgagatgctg tttaacgagg tctacgccca ggtctatgac cgggtgcttc gtgaaaaaca
     7141 gcgggagccg gaagcatgaa caggaaggcg tttctggcct gcgtgctgat gtgcgtcata
     7201 ttaaccggct gcgaaacggc gaaaaaaatc agtgaggtga tcaaaaaccc ggatatccag
     7261 gtcgggagcc tgaaggagca accttcggaa ataaccgtca ccctgctgac cgaaccggac
     7321 accaatacca atgcggaagg tgaatcagcc gccgtggatg tgcaactggt gtacctgact
     7381 gacgactcaa aattgctggc agccgactat gaccagatag ccagcacgcc gctaccggat
     7441 gtgctgggta aaaactatat cgaccaccag gatttcaacc tgttgccgga caccatcaaa
     7501 accctgccgc cggtaaagct ggatgagaaa acgcagttta ttggtgtcgt ggcctatttc
     7561 tccgatgacc aggccactga atggaaacaa atcgagacgg tggaaggaat cggccaccat
     7621 taccgcctgc tggtgcatgt acgtcaaagc agcatcgaaa tgaaaaaaga ggatgaataa
     7681 ttatggcaac cacccgcaac aaggtgatgt ggcaggaagg gatgctgatg cgtccacacc
     7741 atttccagca gcagcagcgt tacaacgact acctggataa ccagcgtttc cgggccatga
     7801 atgatttatc ctggggattt accgaactca ccctcaacaa tgaactgctg gcgcagggta
     7861 agatcatgat tgacagcgcg tcaggcacac tgcccgacgg caccgtcttt tctatccccg
     7921 accaggacgc actgcccgat ccgctgcacc cgcaaaattt tccggacgaa agaagccgca
     7981 atatctacct cgctctgccg gtcgccagtg atgtgagaaa tgaaatcagc gacggacggc
     8041 gaatcgggcg ttaccggctg aattatgccg atgtccggga tttgcattca gaagaaggtg
     8101 acgcgcgaac attgacgctg ggtcaactga cgccgcgcat tatgagcggt gcagaagata
     8161 tgagcgccta tattacgctc ccgctttgcc gtatcagcga tcgccatgcc gatggctccc
     8221 tgacgttgga tgacgatttt atcccctcct gccagaatat tcaggtcagt aagaaactgc
     8281 gtgtttatct caaagaggta cagggggcca ttggcggacg ggcaagcgat ctggcaaacc
     8341 gtattggctc tccggcgcag agcggcatcg cggatgtggc ggaatttatg atgttgcagt
     8401 tacttaaccg taaccagacc cggtttaccc atcacgcccg tcgatcccag ctccacccgg
     8461 aagatttcta ccttgatctt gccgggttgc tgggtgaact gatgaccttt accgagccgt
     8521 cgcgcctgcc ctgcccgctt gatgtgtatg atcatcatga cctgaccaaa atatttaaaa
     8581 cgctgttacc ggaagtcaaa cgggcgctgc ataccgtact gtcgccaaga gccgtcaatc
     8641 tgccgctgca tctgcgtgac ggtatctggc aggccgatat ccatgacaca gaactgctgc
     8701 aatctgccac ctttgtgctg gccgtggcgg caaatatgcc ggtcgatcag atccagcgcc
     8761 agtttatcca gcagtcgaaa atttcctcgc cggaaaaaat ccgcaatatg gtcagtgtgc
     8821 agatcccctg tattccattg cgtgccctta tggtggctcc ccgccagctt ccttaccatt
     8881 ccgggttcag ctatttcgaa ctcgacaaga gtggccaggc ctggacagaa atggctgccg
     8941 ccggggccgt tgcactgcat gtttccggca gtttcccgga tctgaacatg caactgtggg
     9001 cgataagagg gtaagacgat ggatgagggg tcactgtctc tgccaccatt taccggacac
     9061 gacgaaaaaa gccagcgcaa ctaccatctc gcgcttcgtg gcaacagcct gaatccgatg
     9121 atcgacgcgg ccacgccgct actcggtatg gtaatgcgcc tttcaaccat gaacagccag
     9181 acgatgccgg agcatctttt tgctcaggtg gtgacagatg ttcaggccgt ggagcaactg
     9241 cttcaggagc agggctacga accgggcgtg attatctcgt ttcgctacat cctctgcacg
     9301 tttattgatg aagcggcgct cggtaacggc tggtcaaaca agaacgagtg gatcaagcag
     9361 tcattgctgg tccatttcca caacgaagcc tggggtggcg agaaagtttt catcctgctg
     9421 gagcgcctga tccgtgaacc caaacgttac caggatctgc tggaatttct ctggatctgt
     9481 ttttctctgg gatttcgcgg gcgctacaaa gtggcagcac aggatcaggg ggaatttgag
     9541 cagatctacc gccgtctgta ccacgttctg cataaactgc gcggcgatgc ccctttcccg
     9601 ctgttacacc aggacaaaaa aacccagggc gggcgttatc agctcatcag tcgcctgact
     9661 gttaaacata ttttttgtgg tggcgttgtc gtgctggcgt tgttttacct gttctatctg
     9721 ttgcggctgg acagccagac gcaggacatc ctgcaccagc taaacaaatt acttcgataa
     9781 ggacgtttat gatccagatt gatcttccca cgctggtaaa acggctgaac ctgttctccc
     9841 gccaggcgct ggagatggcc gcctctgaat gtatgagtca gcaggcagcg gaaattaccg
     9901 tcagccatgt gctcattcag atgctcgcca tgccacgcag tgacctgcgg gttattaccc
     9961 gccagggcga tattggcatg gaagagttgc gccaggcgct gacggtagag aactacacaa
    10021 ccgcccgttc tgcggacagc tacccggcgt tttccccgat gctggttgag tggcttaaag
    10081 agggctggct gctggcgtcg gctgagatgc agcacagcga actgcgcggc ggcgtgttgc
    10141 tgctggccct gctgcattcg ccgctgcgtt atataccgcc tgctgccgcc cggctgttga
    10201 ccggcattaa ccgtgaccgt ctgcaacagg actttgtgca gtggacacag gagtcggcgg
    10261 aatcagtcgt gccggatgca gacggtaaag gcgcaggcac aatgacggac gcctctgaca
    10321 ccctgcttgc ccgctatgcc aaaaacatga ccgcagacgc ccgtaacggc aggcttgacc
    10381 cggtactgtg ccgcgaccac gaaatcgacc tgatgatcga cattctctgc cgccgccgta
    10441 aaaacaaccc ggtggtggtg ggcgaagcgg gcgtgggcaa aagcgcactg attgaagggc
    10501 tggcgctgcg catcgtggca ggccaggtgc cggacaagct gaaaaacacc gatatcatga
    10561 cccttgactt gggcgcattg caggccgggg cgtcggtgaa gggtgaattc gaaaaacgtt
    10621 tcaaagggct gatggcggag gtcatttcct ccccggtgcc ggtcattctg tttatcgacg
    10681 aagcacatac cctgattggc gcgggcaacc agcagggcgg gctggatatc tccaacctgc
    10741 tcaaaccggc gctggcgcgc ggcgagctga aaaccatcgc cgccaccacc tggagcgagt
    10801 acaaaaaata cttcgaaaaa gatgccgccc tgtcgcgccg cttccagttg gtgaaggtca
    10861 gcgaacccaa cgctgccgaa gccaccatta ttctgcgcgg tctgtcggcg gtctatgaac
    10921 agtctcacgg cgtgctgatt gatgatgacg ccttgcaggc cgctgcgaca ttaagcgagc
    10981 gttatctctc cgggcgtcag ttaccggaca aagcgattga tgtgctggat accgcctgcg
    11041 cccgtgtggc catcaacctg tcgtcgccgc cgaagcaaat ctcggcgctg accactctga
    11101 gccaccagca ggaggcggaa attcgccagc ttgagcgcga gcttcgcatc ggactgcgta
    11161 ccgacacatc acggatgacc gaggtgctgg tgcagtatga tgaaacgctg acggcgctgg
    11221 atgaactgga agcggcctgg caccagcagc agacgctggt ccgggagatt atcgcgctgc
    11281 gccagcagtt actgggcgtg gcagaggacg atgcggcgcc gttgccggac gcagataccg
    11341 tggaggatac gcagccagag tcagagtcag agtcagagtc agagtcagag caggataata
    11401 ccggtgccga accggctgat gaggccgaca gagaacaacc ggaagagacc gctgaaacag
    11461 tttccccggt acagcggctg gcccagctca ctgccgaact ggacgccctg cataacgacc
    11521 ggttgctggt ctccccgcac gtcgataaaa aacagattgc ggcggtgatt gccgaatgga
    11581 ccggcgtgcc gctcaaccgc ctgtcgcaga atgaaatgtc ggtcatcacc gacctgacga
    11641 aatggctggg tgacaccatc aaaggccagg acctggcgat tgccagcctg cataagcatc
    11701 tactgaccgc acgcgccgac ctgcgtcgtc cgggacgccc gctcggtgcg ttcctgctgg
    11761 ctggccccag cggtgtgggt aaaaccgaaa ccgtcctgca actggcagag ctgctctacg
    11821 gcggtcgcca gtacctgacc accatcaata tgtccgaatt ccaggagaaa cacaccgtct
    11881 cgcggctgat tggttcgcct ccgggctacg ttggctacgg tgaaggcggc gtactgaccg
    11941 aagcgattcg ccagaaaccc tactcggtag tactgctcga tgaagtggaa aaagcgcacc
    12001 cggatgtgct caacctgttc taccaggcgt tcgataaggg cgaaatggca gacggtgaag
    12061 gccgcctgat tgactgcaaa aatatcgtct tcttcctgac gtccaacctc ggctaccagg
    12121 taatagtcga gcatgccgat gacccggaaa ccatgcagga agtactgtat ccggtgctgg
    12181 ccgacttctt caaacctgcc ctgctggcgc gtatggaagt ggtgccgtat ctgccgctgt
    12241 cgaaagagac gctcgccacc attatcgccg gaaaactggc ccgtctggat aacgtgctgc
    12301 gcagtcgctt tggtgtagaa gtggtcattg aaccggaagt gacggacgaa atcatgagcc
    12361 gcgtcacccg cgcggaaaac ggcgcgagga tgctggaatc ggtcatcgat ggcgacatgc
    12421 taccgccgct ctcgctgctg ctgttgcaga aaatggcggc taacacggcg attgcccgga
    12481 ttcggttgtc ggcagtggac ggcgcattta cggcagacgt ggaagatgct cagaacgacg
    12541 agtccgtcac aaaggatgaa acggttttat gagcaggttg cgcgttttcc ggcagacagg
    12601 atggtttatc gcgggcctga tgacgggctt acccgccact gccgcgcccg cagaagcatc
    12661 gtcaatggcc ggtgtggcgg tcgccgtcgc caccacgacc ccgccggatg cgacagccac
    12721 gttacaggcc atgcagtcct gtcggcggga gtccgcggcg ctggagcggc ttgactgcta
    12781 tgaccgcctg ctggcaccgc tgtccccgtc agggtttgac ggggcgctgg tcaaagccgg
    12841 ttttgtgggc gaggcctgga cccgcgccac agagcaggaa aaacgccgcg agggcaacac
    12901 cacagaactg ctggtgacgc aggtgccggg ggagcgcccg acggtggtga taaccacccc
    12961 ggctatcggt catgttccgc cacgcccggt gctgatgttc agttgcgtgg acaatatcac
    13021 ccggatgcag gtggcgctga tgcaccctct ggatgtccat gatatcgccg tcactctgaa
    13081 tgccgacaac cgggcattgc gcagccactg gtttgtgcgc gagaacggca ccctgctgga
    13141 gtccagccgg gggttatccg ggattgatga aattaagcaa ctgttcggcg caaaaacgct
    13201 gacggtggat accggcgcag acaatgccgc cggaaagctg acctttaaca ttgacgggct
    13261 ggcaagggcg attgctcccc tgcgcgatgc ctgccactgg gcgggagaat aaaaatatgg
    13321 caatggactt acgcgatccg aatgtctgga tatcgcacct gctggaaaac ctgccggaag
    13381 agaaactggc atcggcgctg aaagatgaca acccgaactg ggagtatatc gacggcgaaa
    13441 tcgtcaagct gggatcgctg gcccacagtc aactggatat cccggaactc cagcgccggg
    13501 ggctggtcat tctggcttcg gagagtaagg attttcgtct gctggcgcat ctgctaagaa
    13561 ccctgcaaca cgccggtgat cctcatctcg cattgcgcct gctggcgctg tatgtggagc
    13621 attactgggc ggtggctgcg ccgcagaata tggcgcataa aaaacgcttt gccagccagg
    13681 tcattaaacg ctttgaaaca ggtattgaag ttttttcaca gaacgccgcc acagcgcagc
    13741 gcgatgcgct gtcagggcaa ctggcaaaac tggcgcaatg ctggcagtca cataacgccc
    13801 cggaactggc acaggctact gatgatcttt ttgccctgta ccagcgtgcg tttaatcgtg
    13861 cggcaccggc tcctgtcccc actccggcgg cctccggtag ttcaccacag accaccgcca
    13921 cgtctgaaag cggcgtgacg caacccagtg ctccggctcc ccaaatcgtc atcgacagtc
    13981 acgacgacaa agcctggcgc gacacgctgt taaaagtggc ggccatttta tgtgaacgcc
    14041 agccggattc accgcagggc taccgtctgc gccgccatgc gctttggcag aacatcacca
    14101 gtacgcccca ggcggaaagc gatggccgta ctccactggc tgcggtctcc gccgatatgg
    14161 tggcagatta ccatgctcag ctcggtagtg ccgatatggc gctgtggcag caggtggaga
    14221 aaagcgtact gctggctcct tactggctgg atggtcactg cctttctgca cagacggcgc
    14281 tgcgtctggg ttacaaacag gtggcagacg ccatccgcga tgaggtcatc cgcttccttg
    14341 agcgtctgcc ccagcttacc gggctgctgt ttaatgacca cacaccgttt atcagtgagc
    14401 agacgaaaca atggctggca gcttcgcccg acgccaaagt tgcaccggtt gcgcaaatcg
    14461 gtgaggaatc gaaggcagcc agagcctgtt ttgctgagca gggtctggag gcggcgctgc
    14521 gatatctgga catgctaccc gaaggcgatc cccgcgatca gtttcaccgc cagtaccttg
    14581 ccgcacagtt gacggaagag gcggggctgg tacagcttgc gcagcagcag taccggatgt
    14641 tgttcaggat gggattgcag atgatggtgg cagactggga gccgtccctg cttgagcagc
    14701 tagaacaaaa attcacggca gaacaataaa agagaacagg agtttttgtg ttcagattac
    14761 caacaccccg actactcagc ggactcaaat cagccctgcg accggcgatg ccccggttta
    14821 aagtctctgc tttctggctg ctgatactgg cgtggatttt tctgctggtg tggatctggt
    14881 ggaaaggccc gatgtggacg ctgtatgaag aacagtggct caaaccactg gcgaaccgct
    14941 ggctggcaac ggcggcgtgg gggattattg ccctggtgtg gcttaccgtc cgggtgatga
    15001 agcgcctgca acagcttgaa aaaatgcaaa agcaacagcg cgaggaagcc gttgatccgc
    15061 tcagcgtgga actgaacgcc cagcagcgtt atcttgaccg ctggctgctg cgcctgcaac
    15121 gccatctcga caaccgccgt ttcctgtggc agttgccgtg gtatatggtc atcggcccgg
    15181 cgggcagtgg caaaacgaca ctgctgcgca aggggtttcc gtccgacatt atttatgccc
    15241 cggagggcgc acgcggcgca gaacaacgcc tgtacctcac gccccatgtc ggtaaacagg
    15301 cggtgatctt tgatatcgac ggcacactct gcgctcccgc tgatgcggat atcctgcatc
    15361 gccgtctgtg ggaacatgcc ctcggctggc tgaaagaaaa gcgcgcgcgc cagccgctga
    15421 acgggattat tctgacactc gatttacccg atctgctgac cgcagacaaa cgccgccgcg
    15481 agcatctgtt acagacgctg cgcagccgct tacaggatat acgccagcat cttcactgcc
    15541 agttaccggt ttacgtggta ctgacccggc ttgatttatt acagggtttt gccgccctgt
    15601 tccagtcact caacagacag gatcgcgatg cgattctggg cgtcacgttc acccgccgtg
    15661 cccatgaaaa tgatgactgg cgaacagagt tgaatgcttt ctggcagaca tgggtggatc
    15721 gaatgaatct ggcgttgccg gatctgatgg tcgctcagac tcacacccgc gcgtctttat
    15781 tcagtttttc ccgccagatg cagggaagcc gtgaaccgct ggtgtcactg cttgagggtc
    15841 tgcttgatgg cgaaaatatg aacgtgatgc tgcgtggtgt ctatctcacc tcttcgcttc
    15901 agcgtggaca gatggatgat atattcaccc agtctgccgc ccgccagtac cggctgggca
    15961 ataacccact ggcgtcctgg cccctggtgg acaccgcgcc ttatttcacc cgcagcctgt
    16021 tcccgcaggc attactcgca gagcctaatc tggcaacaga gagccgcgcc tggctgatac
    16081 gttcccgtcg ccgcctgacg gttttctctg ccacaggcgg cgtggcagca ctgctgctca
    16141 tcaccggctg gcatcactat tacaacggta actatcagtc cggcatcacc gtgcttaagc
    16201 aggccaaagc ctttatggac gtgccgcctc cgcaggggga agatgacttt ggcaacctgc
    16261 aactgccgct cctgaacccg gtacgcgatg ccacactggc ctatggcgac tggggcgacc
    16321 gcagccgtct ggccgatatg ggactgtacc agggacgacg tatcgggcct tatgtggaac
    16381 agacctatct gcaactgctg gagcaacgtt acctgccctc gctgtttaac gggctggtca
    16441 aagcgatgaa cgccgcgccg ccggagagtg aagaaaaact cgcggtgctg cgcgtgatgc
    16501 gaatgctgga ggacaaaagc ggacgtaaca atcaggtggt gaagcagtat atggcaaaac
    16561 gctggagcga aaagttccac ggtcagcgcg atatccaggc acaactgatg tcccatcttg
    16621 actacgcgct ggctcatact gactggcacg cagagcgtca ggcgggcgac ggtgacgcca
    16681 tcagccgctg gacgccatat gacaagcccg tggtatcagc acagaaagaa ctgagcaaac
    16741 tgcctgtcta ccagcgggtt taccagagcc tgaaaacgcg ggcgctgggc gttcttcctg
    16801 ccgacctcaa tctgcgtgac caggtagggc caacctttga ccaggtgttt acatctgccg
    16861 atgacaacaa actggttgtt ccacagttta tcacccgcta cggcctgcaa agctattttg
    16921 taaaacagcg cgatgaactg gttgaactga cagcgatgga ttcctgggta cttaacctca
    16981 cccgcaatgt gaaatacagt gacgccgacc gtgcggaaat ccagcaccag ttgaccgagc
    17041 agtatatcag cgactacacc gccacctggc gggctgggat ggacaatctg aatatccgca
    17101 attttgagtc catcggacaa ctgaccgggg cgctggagca ggttatcagc ggcgaccagc
    17161 ctttgcagcg ggcgctgacc gtgctgcgtg acagcacaca gccaggcgtc ttttctgaaa
    17221 aactctctgc caaagaacgg gaggaagccc tggcagagcc ggattaccag ttactcaccc
    17281 gcctcgggca tgaattcgcc ccggaaaaca gtaccctggc agtacagaaa gacaaagaaa
    17341 gcacgatgca ggccgtgtat cagcaactca ccgagttgca ccgctacctg ctggcaatcc
    17401 agaacgcgcc tgtaccaggg aaatcggcgc tgaaagccgt gcagttacgg cttgatcaga
    17461 acagcagcga tccgatattc gccacccgcc agatggcaaa aacgctgcct gctccgctca
    17521 accgctgggt tggcagactg actgactagg cctggcatgt ggtgatggtg gaggctgttc
    17581 attatatgga agtggactgg cgcgacagcg tggtgaaacc gtttaacgag caactggcaa
    17641 ataactatcc gtttaatccg cgttctgcac aggatgcctc actggatgcc ttcgaacgct
    17701 tctttaaacc ggatggcata ctggatacct tctaccagca gaacctgaag ctgtttatcg
    17761 ataatgacct gagtctggag gatggcgata acaacgtcat tattcgcgaa gatattattg
    17821 cgcaactgga aactgcgcag aaaatccgtg acatcttctt cagcaaacag aacggtctgg
    17881 gaacatcctt tgccgtggaa acggtatcgc tttcaggcaa taaacgccgc agtgtactga
    17941 accttgacag tcagttagtc gattacagcc agggccgtaa ctataccgcc catctggtct
    18001 ggcctaacaa catgcgcgaa ggcaacgaaa gtaagctgac gctcatcggc accagcggca
    18061 acgcgccgcg cagtatcagc ttcagcgggc cgtgggcgca gttccgcctg ttctgggccg
    18121 gacaactgac cggagtacag gatggcaact ttaccgtgcg ctttagcgtg gacggtggcg
    18181 cgatgaccta ccgtgtgcat accgacacgg aagataaccc gttcagcggt gggttgttca
    18241 gccagtttgg tctgtcagac acactgtact gaggaggcag ggatgaatag taacgtactg
    18301 acacaaacta tcgttaccgg cagtgacccg cgcgggctgc cggaattcag cgccatccgc
    18361 gaggaaataa acaaagccag ccacccgtca cagcctgagc tgaactggaa actggtggag
    18421 tcgctggcgc tggcgatttt taaagccaac ggtgtggatt tacacaccgc cacctactat
    18481 acgcttgccc ggacacggac acagggactg gcgggattct gcgaaggtgc ggaactgctg
    18541 gcggcaatgg taagccacga ctgggataag ttctggccgc agggcggccc ggcgcgtact
    18601 gaaatgctgg actggtttaa ctcccgaacc ggcaatattc tgcgtcagca aatctccttt
    18661 gcggaatccg acctaccact gatctaccgc acagagcggg cattgcagct tatctgcgat
    18721 aagctccagc aggtggaact gaagcgcgtt ccgcgcgtgg agaatctgct ctattttatg
    18781 cagcacacgc gtaaacggct tgaaccccag ccgaagagta acgctgagaa cgccgcacag
    18841 accacggtca gaacgctgat ttatgccccg gaaacactgg catcttccac accagaagcg
    18901 gtagtgcctc ccctgcccgg cctgcctgag atgaaagtgg aagtgcgcag tctgacagag
    18961 aatcccccac aggccagtgt gataaagcaa ggcagtacgg taagagggtt tatcgcaggg
    19021 atcgcctgtt cagtggctgt cgcctcagca ttgtggtggt ggcaggtcta tccggtgcag
    19081 cagcaactgt tacaggttaa cgacaccgct cagggcgcag caacggtgtg gatggcctca
    19141 cctgaactcg aaaactatga gcgcaggctg caacaacttc ttgatacctc cccggtacag
    19201 ccgctggaaa ccgggatgca gatgatgcgt gttgccgaca gtcgctggcc ggaaagcctg
    19261 caacagcaac aggcctcgac acaatggaat gaggcactca aaacccgcgc acagagtagc
    19321 ccgcagttgc gtggctggtt gcagacccgc caggacttac atgcttttgc agatctagtg
    19381 atgcagcgcg agaaagaggg actaaccctt tcctatatca aaaatgtcat ctggcaggcg
    19441 gagcggggac tggggcagga aacccccgtt gagtctctgt tgacgcagta ccatgatgcc
    19501 cgtgcgcaga agcagaatac agatgcgctg gaaaaacaaa ttaatgagcg actcgaaggc
    19561 gtgttaagcc gctggctgct gctgaagaat aacgtcatgc cagaggcggc aaccggtacc
    19621 acggccgaaa aataattatt taattaaagg agattatcat ggcaaatata agttatttat
    19681 ctttgagtgg agagacacaa gggctaattt cagcaggttg ctcaacgctt gattctgtcg
    19741 gaaacaaagc gcagccagaa cataaagatc atggtttatg cgttaatgca ttccatctca
    19801 agaagtcaaa atgtcaatca tcatgaatta attatcacca aaccggttga taaatcctca
    19861 ccattgctgg caaaagcact tagtgacaat gaaaaaatgg caatatgtga atttatactc
    19921 taccgaacca gtaaagcggg tatttaccag ccttattata aaataaatct gtcaaaagcc
    19981 cgaatatcaa gtattgattt tgtcacaccg catgctgtcc tggaaaaaga actggaacca
    20041 caggagagaa ttgcctttat ttatgaagat atttcctggg agcatacgtt agccggtacg
    20101 aatgcaatga gtaaatggca ggatcgtgta cagtaactta ctgtaataac ataataaatt
    20161 actattgcgg agagataaaa tggctggttt aaggaataac aacaacacac agaatgcaca
    20221 gtgggctgat tatgtgggtg acattcttcg gggggctcag ccgataaacc agctagtacc
    20281 tcagcaccct tatcttaacg acgtcccact cattgatgag ttaaggcatc agaacactca
    20341 tcatgtt
//
LOCUS       DAZPDU010000068        20260 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZPDU010000068 DAZPDU010000000
VERSION     DAZPDU010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20260
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.069"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(311..1123)
                     /locus_tag="ACHURS_002229"
     CDS             complement(311..1123)
                     /locus_tag="ACHURS_002229"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFR1237947.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTLDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHAASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPILGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGEKRS"
     gene            1385..2146
                     /gene="udp"
                     /locus_tag="ACHURS_002230"
     CDS             1385..2146
                     /gene="udp"
                     /locus_tag="ACHURS_002230"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFR1237948.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGIMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            2287..3714
                     /gene="rmuC"
                     /locus_tag="ACHURS_002231"
     CDS             2287..3714
                     /gene="rmuC"
                     /locus_tag="ACHURS_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFR1237949.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQNEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            3809..4564
                     /gene="ubiE"
                     /locus_tag="ACHURS_002232"
     CDS             3809..4564
                     /gene="ubiE"
                     /locus_tag="ACHURS_002232"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFR1237950.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            4578..5183
                     /gene="ubiJ"
                     /locus_tag="ACHURS_002233"
     CDS             4578..5183
                     /gene="ubiJ"
                     /locus_tag="ACHURS_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFR1237951.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            5180..6820
                     /gene="ubiB"
                     /locus_tag="ACHURS_002234"
     CDS             5180..6820
                     /gene="ubiB"
                     /locus_tag="ACHURS_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFR1237952.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVATLEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            6899..7168
                     /gene="tatA"
                     /locus_tag="ACHURS_002235"
     CDS             6899..7168
                     /gene="tatA"
                     /locus_tag="ACHURS_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFR1237953.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            7172..7687
                     /gene="tatB"
                     /locus_tag="ACHURS_002236"
     CDS             7172..7687
                     /gene="tatB"
                     /locus_tag="ACHURS_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFR1237954.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            7690..8466
                     /gene="tatC"
                     /locus_tag="ACHURS_002237"
     CDS             7690..8466
                     /gene="tatC"
                     /locus_tag="ACHURS_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFR1237955.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            8508..9290
                     /gene="tatD"
                     /locus_tag="ACHURS_002238"
     CDS             8508..9290
                     /gene="tatD"
                     /locus_tag="ACHURS_002238"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFR1237956.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVYDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTRFGIAF"
     gene            complement(9287..9775)
                     /gene="rfaH"
                     /locus_tag="ACHURS_002239"
     CDS             complement(9287..9775)
                     /gene="rfaH"
                     /locus_tag="ACHURS_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFR1237957.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASLAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            9942..11435
                     /gene="ubiD"
                     /locus_tag="ACHURS_002240"
     CDS             9942..11435
                     /gene="ubiD"
                     /locus_tag="ACHURS_002240"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFR1237958.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            11482..12183
                     /gene="fre"
                     /locus_tag="ACHURS_002241"
     CDS             11482..12183
                     /gene="fre"
                     /locus_tag="ACHURS_002241"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFR1237959.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(12373..13536)
                     /gene="fadA"
                     /locus_tag="ACHURS_002242"
     CDS             complement(12373..13536)
                     /gene="fadA"
                     /locus_tag="ACHURS_002242"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFR1237960.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRARELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(13546..15735)
                     /gene="fadB"
                     /locus_tag="ACHURS_002243"
     CDS             complement(13546..15735)
                     /gene="fadB"
                     /locus_tag="ACHURS_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFR1237961.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLSMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            15925..17256
                     /gene="pepQ"
                     /locus_tag="ACHURS_002244"
     CDS             15925..17256
                     /gene="pepQ"
                     /locus_tag="ACHURS_002244"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFR1237962.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHASVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            17256..17870
                     /locus_tag="ACHURS_002245"
     CDS             17256..17870
                     /locus_tag="ACHURS_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFR1237963.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLMTQRKTPLTEYTLQCEYHQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            17909..19360
                     /gene="trkH"
                     /locus_tag="ACHURS_002246"
     CDS             17909..19360
                     /gene="trkH"
                     /locus_tag="ACHURS_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFR1237964.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            19372..19917
                     /gene="hemG"
                     /locus_tag="ACHURS_002247"
     CDS             19372..19917
                     /gene="hemG"
                     /locus_tag="ACHURS_002247"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFR1237965.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAQLTNKPTLK"
BASE COUNT         4750 a         5114 c         5504 g         4892 t
ORIGIN      
        1 tgaactaaaa tgtgaaaaac aaaatgtttc actttaatat ctttttatca ggaaggtctg
       61 agatgattta taattaatgt gaatgtgttg ataataatta atgtttgttt gtgaatggga
      121 gttgatggta aatctctaag gtgtcttcca ttgaagagac tcatttatga ctatgacttg
      181 ccgactttct taaggtcaaa tagcggagtc gtaatctcca atcaattcat actgattatt
      241 ttttggggga cagagatggc agcaaaacgc actcgtttac tcaccatctc aaatatagac
      301 taagaggaaa ctaactccgc ttctctccat actgcttaaa ccattccagc atacgctgcc
      361 agccatcttt cgcagattcg gcatgatagc tcgggcgata atcagcgttg aatgcatgcc
      421 cggcatccgg gtacacgata atctctgctt tcgcattagc agcccgcagc gcctggcgca
      481 tggtttcaac gctctcctgc ggaatgctgt tatcctgacc gccatataag ccgagaatcg
      541 gcgcgttaag atcggttgcg atatcaacag gttgtttcgg tgaattcagc gacttgtcgc
      601 ccgtcagttt gccgtaccac gccactgcag cttttagctg tggattatgc gcggcataca
      661 gccaggtgat acgtccaccc cagcagaatc cggtgatcat taaacgatga acatcgccgc
      721 cgttgcggga agcccaactg gcggcatgat cgagatcggc cagcacctgc gagtcaggca
      781 cttttgctac cagaccgcta agcaacgtgg ggatatcggc aaaatcattc ggatcgcctt
      841 cgcggaagta aagttcaggt gcgatagcca gatacccctc cagcgccaga cggcgacaaa
      901 tgtcgcggat atgttcatgc acgccaaaaa tttcctgcac tacaatgacc actggcagtg
      961 ggccatcgct ttgctttggt ctggcatggt aagcaggcat gttatcccct tgtgaaggga
     1021 tagaggtgaa gcccgccaca atcgcgtcgt ccagggtctg aacgatggtc gaagcgagag
     1081 gcgatgcagc aggtgcaaat ccagattgtt gtgttgttgc catggtattc tccgtaccct
     1141 tataaaaatg ttgcgcaatg ttaactatag tcagcatgca acaaatcaca ttgcctgaat
     1201 cggctcatct tttatgcagt cctgcagaat gaagagtgat ttatgtgatt tgaatcactt
     1261 ttggtgggta aatttatgca acgcatttgc gtcatggtga tgagtatcac gaaaaaatgt
     1321 taaacccttc ggtaaagtgt ctttttgctt cttctgacta aaccgattca cagaggagtt
     1381 gtatatgtcc aagtctgatg tttttcatct cggcctcact aaaaacgatt tacaaggggc
     1441 tacgcttgcc atcgtccctg gcgacccgga tcgtgtggaa aagatcgccg cgctgatgga
     1501 taagccggtt aagctggcat ctcaccgcga attcactacc tggcgtgcag agctggatgg
     1561 taaacctgtt atcgtctgct ctaccggtat cggcggcccg tctacctcta ttgctgttga
     1621 agagctggca cagctgggca ttcgcacctt cctgcgtatc ggtacaacgg gcgctattca
     1681 gccgcatatt aatgtgggtg atgtcctggt taccacggcg tctgtccgtc tggatggcgc
     1741 gagcctgcac ttcgcaccgc tggaattccc ggctgtcgct gatttcgaat gtacgactgc
     1801 gctggttgaa gctgcgaaat ccattggcgc gacaactcac gttggcgtga cagcttcttc
     1861 tgataccttc tacccaggtc aggaacgtta cgatacttac tctggtcgcg tagttcgtca
     1921 ctttaaaggt tctatggaag agtggcaggc gatgggcata atgaactatg aaatggaatc
     1981 tgcaaccctg ctgaccatgt gtgcaagtca gggcctgcgt gccggtatgg tagcgggtgt
     2041 tatcgttaac cgcacccagc aagagatccc gaatgctgag acgatgaaac aaactgaaag
     2101 ccaagcggtg aaaatcgtgg tggaagcggc gcgtcgtctg ctgtaattct cttctcctgt
     2161 ctgaaggccg acgcgttcgg ccttttgtat ttttgcgtag cgcctcgcag gaaatgcctt
     2221 tccaactgga cgtttgtaca gcacaattct attttgtgcg ggtaagttgt tgcgtcagga
     2281 ggcgttgtgg atttctcaat catggtttac gcagttattg cgttggtggg tgtggcaatt
     2341 ggctggctgt ttgccagtta tcaacatgcg cagcaaaaag ccgagcaatt agctgaacgt
     2401 gaagagatgg tcgcggagtt aagcgcggca aaacaacaaa ttacccaaag cgagcactgg
     2461 cgtgcagagt gcgagttact caataacgaa gtgcgcagcc tgcaaagtat taacacctct
     2521 ctggaggccg atctgcgtga agtaaccacg cggatggaag ccgcacagca acatgctgac
     2581 gataaaattc gccagatgat taacagcgag cagcgcctca gtgagcagtt tgaaaacctc
     2641 gccaaccgta tttttgagca cagcaatcgc cgggttgatg agcaaaaccg tcagagtctg
     2701 aacagcctgt tgtcgccgct acgtgaacaa ctggacggtt tccgccgtca ggttcaggac
     2761 agcttcggta aagaagcaca agaacgccat accctgaccc acgaaattcg caatctccag
     2821 caactcaacg cgcaaatggc ccaggaagcg atcaacctga cgcgcgcgct gaaaggcgac
     2881 aataaaaccc agggcaactg gggcgaggta gtattgacgc gggtgctgga ggcttccggt
     2941 ctgcgtgaag ggtatgaata tgaaacccag gtcagcatcg aaaatgacgc ccgctcgcgg
     3001 atgcagccgg atgtcatcgt gcggctgccg cagggaaaag atgtggtgat cgacgccaaa
     3061 atgacgctgg tcgcctatga acgctatttt aacgccgaag acgactacac ccgcgaaagc
     3121 gcgctacagg aacatatcgc gtcggtgcgt aaccatatcc gtttgctggg acgcaaagat
     3181 tatcaacagc tgccggggct gcgaactctg gattacgtgc tgatgtttat tcccgttgaa
     3241 cccgcttttt tactggcgct tgaccgccag ccggagctga tcaccgaagc gttgaaaaac
     3301 aatatcatgc tggttagccc gactacgctg ctggtggcgt tgcgtactat tgccaacctg
     3361 tggcgttatg agcatcaaag ccgcaacgcc cagcaaatcg ccgatcgtgc cagcaagctg
     3421 tacgacaaga tgcgtttgtt catcgatgac atgtccgcga ttggtcaaag tctcgacaaa
     3481 gcgcaggata actaccgcca ggcaatgaaa aaactctctt caggtcgcgg aaatgtgctg
     3541 gcgcaggcag aagcgtttcg cggtttagga gtagaaatta aacgcgagat taatccggat
     3601 ttggctgaac aggcggtgag ccagaatgaa gagtatcgac ttcggtcggt tccggagcag
     3661 ccgaatgatg aagcttatca acgcgatgat gaatataatc agcagtcgcg ctagcccatt
     3721 aggggtagtt aagccgggta gaaatctagg gcatcgacgc ccaatctgtt acacttctgg
     3781 aacaattttt tgatgagcag gcattgagat ggtggataag tcacaagaaa cgacgcactt
     3841 tggttttcag accgtcgcga aggaacaaaa agcggatatg gtcgcccacg ttttccattc
     3901 cgtggcatca aaatacgatg tcatgaatga tttgatgtca tttggtattc atcgtttgtg
     3961 gaagcgattc acgattgatt gcagcggcgt acgccgtggg cagaccgtgc tggatctggc
     4021 tggtggcacc ggcgacctga cagcgaaatt ctcccgcctg gtcggagaaa ctggcaaagt
     4081 ggtccttgct gatatcaatg aatccatgct caaaatggga cgcgagaagc tgcgtaatat
     4141 cggtgtgatt ggcaacgttg agtatgttca ggcgaacgct gaggcgctgc cgttcccgga
     4201 taacaccttt gattgcatca ccatttcgtt tggtctgcgt aacgtcaccg acaaagataa
     4261 agcactgcgt tcaatgtatc gcgtgctgaa acccggcggc cgcctgctgg tgcttgagtt
     4321 ctcgaagcca attatcgagc cgctgagcaa agcctatgat gcatactcct tccatgtgct
     4381 gccgcgtatt ggctcactgg tcgcgaacga cgccgacagc taccgttatc tggcagaatc
     4441 catccgtatg catcccgatc aggataccct gaaagccatg atgcaggatg ccggattcga
     4501 aagtgtcgac tactacaatc tgacggcagg ggttgtggcg ctgcatcgtg gttataagtt
     4561 ctgacaggag accggaaatg ccttttaaac ctttagtgac ggcaggaatt gaaagtctgc
     4621 tcaacacctt cctgtatcgc tcacccgcgc tgaaaacagc ccgctcgcgt ctgctgggta
     4681 aagtattgcg cgtggaggta aaaggctttt cgacgtcatt gattctggtg ttcagcgaac
     4741 gccaggttga tgtactgggc gaatgggcag gcgatgctga ctgcaccgtt atcgcctacg
     4801 ccagtgtgtt gccgaaactt cgcgatcgcc agcagcttac cgcactgatt cgcagtggtg
     4861 agctggaagt gcagggcgat attcaggtgg tgcaaaactt cgttgcgctg gcagatctgg
     4921 cagagttcga ccctgcggaa ctgctggccc cttataccgg tgatatcgcc gctgaaggaa
     4981 tcagcaaagc catgcgcgga ggcgcaaagt tcctgcatca cggcattaag cgccagcaac
     5041 gttatgtggc ggaagccatt actgaagagt ggcgtatggc acccggtccg cttgaagtgg
     5101 cctggtttgc ggaagagacg gctgccgtcg agcgtgctgt tgatgccctg accaaacggc
     5161 tggaaaaact ggaggctaaa tgacgccagg tgaagtacgg cgcctatatt tcatcattcg
     5221 cactttttta agctacggac ttgatgaact gatccccaaa atgcgtatca ccctgccgct
     5281 acggctatgg cgatactcat tattctggat gccaaatcgg cataaagaca aacttttagg
     5341 tgagcgacta cgactggccc tgcaagaact ggggccggtt tggatcaagt tcgggcaaat
     5401 gttatcaacc cgccgcgatc tttttccacc gcatattgcc gatcagctgg cgttattgca
     5461 ggacaaagtt gctccgtttg atggcaagct ggcgaagcag cagattgaag ctgcaatggg
     5521 cggcttgccg gtagaagcgt ggtttgacga ttttgaaatc aagccactgg cttctgcttc
     5581 tatcgcccag gttcataccg cgcgattgaa atcgaatggt aaagaggtgg tgattaaagt
     5641 catccgcccg gatattttgc cggttattaa agcggatctg aaacttatct accgtctggc
     5701 tcgctgggtg ccgcgtttgc tgccggatgg tcgccgtctg cgcccaaccg aagtggtgcg
     5761 cgagtacgaa aagaccttga ttgatgaact gaatttgctg cgggaatctg ccaacgccat
     5821 tcagcttcgg cgaaattttg aagacagccc gatgctctac atcccggaag tttaccctga
     5881 ctattgtagt gaagggatga tggtgatgga gcgcatttac ggcattccgg tgtctgatgt
     5941 tgcgacgctg gagaaaaatg gcaccaacat gaaattgctg gcggaacgtg gcgtgcaggt
     6001 gttcttcact caggtctttc gcgacagctt tttccatgcc gatatgcacc ctggcaacat
     6061 tttcgtaagc tatgaacacc cggaaaaccc gaaatatatc ggcattgatt gcgggattgt
     6121 tggctcgcta aacaaagaag ataaacgcta tctggcagaa aactttatcg ccttctttaa
     6181 tcgcgactat cgcaaagtgg cagagctaca cgtcgattct ggctgggtgc caccagatac
     6241 caacgttgaa gagttcgaat ttgccattcg tacggtctgt gaacctatct ttgagaaacc
     6301 gctggccgaa atttcgtttg gacatgtact gttaaatctg tttaatacgg cgcgtcgctt
     6361 caatatggaa gtgcagccgc aactggtgtt actccagaaa accctgctct atgtcgaagg
     6421 ggtaggacgc cagctttatc cgcagctcga tttatggaaa acagcgaagc ctttcctgga
     6481 gtcgtggatt aaagatcagg tcggtattcc tgccctggtg agagcattta aagaaaaagc
     6541 gccgttctgg gtcgaaaaaa tgccagaact gcctgaattg gtttacgaca gtttgcgcca
     6601 gggcaagtat ttacagcaca gtgttgataa gattgcccgc gagcttcagt caaatcatgt
     6661 acgtcaggga caatcgcgtt attttctcgg aattggcgct acgttagtat taagtggcac
     6721 attcttgttg gtcagccgac ctgaatgggg gctgatgccc ggctggttaa tggcaggtgg
     6781 tctgatcgct tggtttgtcg gttggcgcaa aacacgctga ttttttcatc gctcaaggcg
     6841 ggtcgtgtaa cgtataatgc ggccttgttt aatcatcatc taccacagag gaacatgtat
     6901 gggtggtatc agtatttggc agttattgat tattgccgtc atcgttgtac tgctttttgg
     6961 caccaaaaag ctcggctcca tcggttccga tcttggtgcg tcgatcaaag gctttaaaaa
     7021 agcaatgagc gatgatgaac caaagcagga taaaaccagt caggatgctg attttactgc
     7081 gaaaactatc gccgataagc aggcggatac gaatcaggaa caggctaaaa cagaagacgc
     7141 gaagcgccac gataaagagc aggtgtaatc cgtgtttgat atcggtttta gcgaactgct
     7201 attggtgttc atcatcggcc tcgtcgttct ggggccgcaa cgactgcctg tggcggtaaa
     7261 aacggtagcg ggctggattc gcgcgttgcg ttcactggcg acaacggtgc agaacgaact
     7321 gacccaggag ttaaaactcc aggagtttca ggacagtctg aaaaaggttg aaaaggcgag
     7381 cctcactaac ctgacgcccg aactgaaagc gtcgatggat gaactacgcc aggccgcgga
     7441 gtcgatgaag cgttcctacg ttgcaaacga tcctgaaaag gcgagcgatg aagcgcacac
     7501 catccataac ccggtggtga aagataatga agctgcgcat gagggcgtaa cgcctgccgc
     7561 tgcacaaacg caggccagtt cgccggaaca gaagccagaa accacgccag agccggtggt
     7621 aaaacctgct gcggacgctg aaccgaaaac cgctgcacct tccccttcgt cgagtgataa
     7681 accgtaaaca tgtctgtaga agatactcaa ccgcttatca cgcatctaat tgagctgcgt
     7741 aagcgtctgc tgaactgcat tatcgcggtg atcgtgatat tcctgtgtct ggtctatttc
     7801 gccaatgaca tctatcacct ggtatccgcg ccattgatca agcagttgcc gcaaggttca
     7861 acgatgatcg ccaccgacgt ggcctcgccg ttctttacgc cgatcaagct gacctttatg
     7921 gtgtcgctga ttctgtcagc gccggtgatt ctctatcagg tgtgggcatt tatcgcccca
     7981 gcgctgtata agcatgaacg tcgcctggtg gtgccgctgc tggtttccag ctctctgctg
     8041 ttttatatcg gcatggcatt cgcctacttt gtggtctttc cgctggcatt tggcttcctt
     8101 gccaataccg cgccggaagg ggtgcaggta tccaccgaca tcgccagcta tttaagcttc
     8161 gttatggcgc tgtttatggc gtttggtgtc tcctttgaag tgccggtggc aattgtgctg
     8221 ctgtgctgga tggggattac ctcgccagaa gacttacgca aaaaacgccc gtatgtgctg
     8281 gttggtgcat tcgttgtcgg gatgttgctg acgccgccgg atgtcttctc gcaaacgcta
     8341 ttggcgatcc cgatgtactg tctgtttgaa atcggtgtct tcttctcacg cttttacgtt
     8401 ggtaaagggc gaaatcggga agaggaaaac gacgctgaag cagaaagcga aaaaactgaa
     8461 gaataaattc aaccgcccgt cagggcggtt gtcatatgga gtacaggatg tttgatatcg
     8521 gcgttaattt gaccagttcg caatttgcga aagaccgtga tgatgttgta gcgcgcgctt
     8581 ttgacgcggg agttaatggg ctactcatca ccggcactaa cctgcgtgaa agccagcagg
     8641 cgcaaaagct ggcgcgtcag tattcgtcct gttggtcaac ggcgggcgta catcctcacg
     8701 acagcagcca gtggcaagct gcgactgaag aagcgattat tgagctggcc gcgcagccag
     8761 aagtggtggc gattggtgaa tgtggtctcg actttaaccg caacttttcg acgccggaag
     8821 agcaggaacg cgcttttgtt gcccagctac gcattgccgc agatttaaac atgccggtat
     8881 ttatgcactg tcgcgatgcc cacgagcggt ttatgacatt gctggagccg tggctggata
     8941 aactgcctgg tgcggttctt cattgcttta ccggcacacg cgaagagatg caggcgtgcg
     9001 tggcgcatgg aatttatatc ggcattaccg gttgggttta cgatgaacga cgcggactgg
     9061 agctgcggga acttttgccg ttgattccgg cggaaaaatt actgatcgaa actgatgcgc
     9121 cgtatctgct ccctcgcgat ctcacgccaa agccatcatc ccggcgcaac gagccagccc
     9181 atctgcccca tattttgcaa cgtattgcgc actggcgtgg agaagatgcc gcatggctgg
     9241 ctgccaccac ggatgctaat gtcaaaacac ggtttgggat tgcgttttag agtttgcgga
     9301 actcggtgtt cttcacactg tgcttaatct ctttattaat aagattaagc aatagcatgg
     9361 agcgagcctc accatcgggt tcggtgaaaa tggcctgaaa gccttcgaac gcgccttcgg
     9421 taataatcac cttatctccc ggataagggg ttgccggatc gacaatgtct ttcggtttat
     9481 ataccgatag ctgatgaatc accgccgatg ggactatcgc tagcgacgcg ccaaagcgca
     9541 cgaagtggct gacaccgcgg gtcgcgttga tagtcgtggt atgaatcact tctggatcaa
     9601 attcgacaaa caggtagttg ggaaacaatg gctcactgac tgcagtacgt tttccacgca
     9661 cgattttttc cagggtgatc atcggtgcca ggcaattcac agcctgtctc tcaaggtgtt
     9721 cctgggcacg ttgaagttgc ccgcgcttgc agtacagtaa ataccaagat tgcataatga
     9781 ctcttatccg tttaatcggg gcgcaaggat agcaaaagct ttacgctaag ttaattatat
     9841 tccccggttt gcgttatacc gtcagagttc acgctaattt aacaaattta cagcatcgca
     9901 aagatgaacg ccgtataatg ggcgtagatt aagaggctac aatggacgcc atgaaatata
     9961 acgatttacg cgacttcttg acgctgcttg aacagcaggg tgagctaaaa cgtatcacgc
    10021 tcccggtgga tccgcatctg gaaatcactg aaattgctga ccgcactttg cgtgccggtg
    10081 ggcctgcgct gttgttcgaa aaccctaaag gctactcaat gccggtgctg tgcaacctgt
    10141 tcggtacgcc aaagcgcgtg gcgatgggca tggggcagga agatgtttcg gcgctgcgtg
    10201 aagttggtaa attattggcg tttctgaaag agcccgagcc gccaaaaggt ttccgcgacc
    10261 tgtttgataa actgccgcag tttaagcaag tattgaacat gccgacaaag cggctgcgtg
    10321 gtgcgccctg ccaacaaaaa atcgtctctg gcgatgacgt cgatctcaat cgcattccca
    10381 ttatgacctg ctggccggaa gatgccgcgc cgctgattac ctgggggctg accgtaacgc
    10441 gcggcccgca taaagagcgg cagaatctgg gcatttatcg ccagcagctg attggtaaaa
    10501 acaaactgat tatgcgctgg ctgtcgcatc gcggcggcgc gctggattat caggagtggt
    10561 gtgcggcgca tccgggcgaa cgtttcccgg tttctgtggc gctgggtgcc gatcccgcca
    10621 cgattctcgg tgcagtcacc cccgttccgg atacactttc agagtatgcg tttgccggat
    10681 tgctgcgcgg taccaagacc gaagtggtga agtgtatctc caatgacctt gaagtgcccg
    10741 ccagtgcgga gattgtgctg gaagggtata tcgaacaagg cgaaactgcg ccggaagggc
    10801 cgtatggcga ccacaccggt tactataacg aagtcgatag tttcccggta tttaccgtga
    10861 cgcatattac ccagcgtgaa gatgcgattt accactccac ctatactggt cgtccgccgg
    10921 atgaacccgc ggtgctgggt gtcgcactga acgaagtgtt tgtgccgatt ctgcaaaaac
    10981 agttcccgga aattgtcgat ttttacctgc caccggaagg ctgttcttat cgtctggcgg
    11041 tagtgacaat caaaaaacag tacgccggac acgcgaagcg cgtcatgatg ggcgtctggt
    11101 cgttcttacg ccagtttatg tacactaaat ttgtgatcgt ttgcgatgat gacgtcaacg
    11161 cacgcgactg gaacgatgtg atttgggcga ttaccacccg tatggacccg gcgcgggata
    11221 ctgttctggt agaaaatacg cctattgatt atctggattt tgcctcgcct gtctccgggc
    11281 tgggttcaaa aatggggctg gatgccacga ataaatggcc gggggaaacc cagcgtgaat
    11341 ggggacgtcc catcaaaaaa gatcctgatg tcgtcgcgca tattgacgcc atctgggatg
    11401 aactggctat ttttaacaac ggtaaaagcg cctgatgcgc gttagttttg ccctatttat
    11461 ttgatccgac agagaaagcg catgacaacc ttaagctgta aagtgacctc ggtagaagct
    11521 atcacggata ctgtataccg tgtccgaatc gtaccagacg cggccttttc ttttcgtgct
    11581 ggtcagtatt tgatggtagt gatggatgag cgcgacaaac gtccgttttc aatggcttcg
    11641 acgccggatg aaaaagggtt tatcgagctg catattggcg cttctgaaat caacctttac
    11701 gcgaaagcag tcatggaccg catcctcaaa gatcatcaaa tcgtggtcga tattccccac
    11761 ggagaagcgt ggctgcgcga tgatgaagag cgtccgatga ttttgattgc gggcggcacc
    11821 gggttctcct atgcgcgctc gattttattg acggcgttgg cgcgtaatcc caatcgcgat
    11881 atcaccattt actggggtgg gcgtgaagag caacatctgt atgatctctg cgagcttgag
    11941 gcgctttcgc tgaagcatcc tggtctgcaa gtggtgccgg tggttgaaca accggaagcg
    12001 ggctggcgtg ggcgtactgg caccgtgtta acggcggtct tgcaggatca tggtacgctg
    12061 gcagagcatg atatctatat tgccggacgt tttgagatgg cgaaaattgc tcgtgacctg
    12121 ttttgcagtg agcgtaatgc gcgggaagat cgtctgtttg gcgatgcgtt tgcatttatc
    12181 tgagatataa aaacccgccc ctgacaggcg ggaagaacgg caactaaaac tttattcagt
    12241 ggcatttaga tctatgtcgc atccggcaat cggtgcacga tgcctgatgc gacgctggcg
    12301 cgtcttatca ggcctacatt ggtgcaggat cggtagaccg gataaggcgt tcacgccgca
    12361 tccggcaagt ggttaaaccc gctcaaacac cgtcgcaata ccctgaccca gaccgataca
    12421 catcgtcgcc agaccaaact gaacgtcttt gtgttccatc agattcagca acgtggtgct
    12481 gatacgcgca ccggaacaac ccagcggatg acccagcgcg atcgcaccac cattgaggtt
    12541 gatcttctcg tcaatctgct ccattagccc gagatcttta atgcacggca ggatctgcgc
    12601 ggcaaaggct tcgttcatct caaacacgcc gatatcgctg gcagaaagcc ccgctttttt
    12661 cagcgccagc ttcgaggcag gaaccgggcc gtaacccata atcgacggat cacaaccaac
    12721 gaccgccatc gaacgcacac gggcgcgcgg cttaagacct aattcacggg cgcggctttc
    12781 actcatcacc agcatggccg cggcaccatc agaaagtgca gaagatgtac ccgccgttac
    12841 cgtaccgctt actggatcaa acgccggacg cagcgtggcg agggcttcca cggtggtttc
    12901 cgggcgaatc acttcgtcgt aattaaactg cttcaggacg ccgtcggcat cgtgaccacc
    12961 ggtcgggatg atttcatttt taaatgcgcc cgactgcgtg gcggcccagg cgcgggcgtg
    13021 tgaccgcgcg gcaaaggcat cctgcatttc acggctgata ccgtgcatac gcgccagcat
    13081 ttctgccgtt aagcccatca tgcccgccgc tttggcgaca ttgcggctca ggccggggtg
    13141 aaaatcgacg ccgtgactca tcggcacatg gcccatatgc tccacgccgc caaccagaca
    13201 tgcctgcgca tcgccagtca tgatcattcg tgctgcgtca tgcagtgcct gcatggatga
    13261 accacacaag cgattgacgg taaccgccgg gacagagtgc ggcacttctg ccagcagcgc
    13321 cgcgttacgg gcgatattaa aaccctgctc cagcgtctgc tgcacacaac cccagtaaat
    13381 atcgtcgagg gccgccgctt ccagcgccgg gttacgcgcc agcaggctac gcattaaatg
    13441 agcggagaga tcttctgcac gcacgttacg aaaagcaccg cccttcgaac ggcccatcgg
    13501 ggtgcgaatt gcatcgacaa tgacaacctg ttccattgtg actccttaag ccgttttcag
    13561 gtcgccaacc ggacgggctg gctcaaccgg aggatagtac ggttcgttat gacgcgcttt
    13621 attacgcaga ccttccggca cttcatacag cgggccgagg tgctgatatt gctgtgccat
    13681 atcgaggtat tttgcgctac cgagggtgtc cagccagcgg aacgcgccgc cgtggaacgg
    13741 agggaagccc aggccgtaga ccagcgccat atccgcttcc gccggagtgg cgataatgcc
    13801 ctcttccaga cagcgcacca cttcgttgac catcgggatc atcatgcggg cgataatctc
    13861 ttcttcgctg aaatcgcgct tcggctggct cacttctgcc agcaggtctt caacggcggc
    13921 gtcttcttct ttcttcggct tacctttgct gtcttcttta taacgccaga aaccgaggcc
    13981 gttcttctga ccaaagcggt tggcatcaaa cagcgcgtcg atggcatcgc ggtaatcttt
    14041 ttgcatccgc tgcgggaagc ctgctgccat gacagcctga gcgtgatgcg cggtatcaat
    14101 gcccacaacg tccagcagat atgccgggcc catcggccag ccaaactgtt tttccatcac
    14161 tttgtcgatc ttgcggaaat ccgcgccgtc gcgcagcagc tggctgaaac cggcgaaata
    14221 cgggaacagc acgcggttaa caaagaagcc ggggcagtcg ttaaccacaa tcggcgtctt
    14281 gcccatcttg ctcgcccagg cgacaacttt cgcgatggtt tcgtcggagc ttttctcgcc
    14341 gcgaataatt tctaccaacg gcattcggtg gaccgggtta aagaagtgca tcccgcagaa
    14401 gttttccggg cgttccagcg cgttggccag ttcgctgata ggaatggttg aagtgttaga
    14461 cgccagcacg gtatcctggc gtactttttg ttcggtttct gccagtacgg cttttttcac
    14521 tttcgggttt tcaacaaccg cttctaccac aatatccacg cggtcaaatc cggcgtagtc
    14581 gagcgttggg tggatcgtgg agatcacgcc agccagtttc agaccatcga tcttgccgcg
    14641 ctcaagctgc ttgttcagca gtttcgcggc ttcggtcatg ctgagggtta acgacttgtc
    14701 gttgatatct ttcatgacaa ccggcacgcc tttccacgcg gactggtaag cgataccgcc
    14761 acccatgatg cctgcgccca gcacagcagc ctgtttcggg gtttcaacgt ctttggtgag
    14821 tttcttcgct ttgcctttta catattgatc gttaaggaaa atgccgacca gtgcgcgagc
    14881 ttcgttggta tgcgccagcg ggacaaaact tttgttttcc agatttaagg cttcttcacg
    14941 accaaaacgg gccgcagctt caatggtttt tactgcggtg atgggggccg gataatgttt
    15001 ccccgccgtt tgcgcgacca ttcctttagc gatggtgaag ctcatggtgg cttcaatctt
    15061 gctcagtttt agcggttcca gcttcggctg gcgtttcgct ttccagtcga ggtcgccgtt
    15121 aatggcctgg cgtaaaaccg cctttgcgcc ttcaaccagt ttttctgctt tgactacccc
    15181 atccaccaga ccgattttca gcgcctgatc cgcgccgaca tctttaccgg cggcaatgat
    15241 ttccagcgca ctgtcagcgc ccagcatacg tggcatacgt acagaaccgc caaagcccgg
    15301 catgatgccc agtttggttt ccggcagacc gatgcgcaga tccggcgtcg ccagacgata
    15361 atcggtcgcc agcacgcatt cgcagccacc acccagcgca tagccgttga cggcagcaat
    15421 ggtcggcacc ggcagatctt ccaggcgatt aaacacgcta ttggcaaagt gcagccactg
    15481 acttaactgt tcttcaggaa cgaggaacag ggacaaaaat tcggtgatat cagcaccgac
    15541 gataaaggct gctttgttcg aacgcagcag cagccctttt agatctgatt gctgttccag
    15601 cacgccgatg gcctcgccga ggctggcgac ggtcgcagtg tcgagtttat taactgaacc
    15661 tggggcatca aataccagtt cggcaatgcc atcttccagc cagtcaaggt acagggtgtc
    15721 gcctttgtaa agcatgtcag tctcctgaat ccgcaaggtg atctggtcgt accagatgca
    15781 tcgaagtgtg tattttgtgt taaaaatatg caaataaaag attaaagaaa tgccgatctg
    15841 atcacgctcg gcagaaatca cgctctggat gaacgatgtg ctaagatgcg gagacttaag
    15901 gtcaaaaaaa cagaagggta aaaaatggaa tcactggcct cgctctataa aaatcatata
    15961 gctaccttac aagaacggac tcgcgatgcg ctggcgcgct tcaagctgga tgcattactt
    16021 attcactccg gcgagctgtt caacgttttt ctcgacgatc atccctatcc gtttaaagtg
    16081 aacccgcaat tcaaagcgtg ggtgccggta actcaggtgc caaactgctg gttgctggtg
    16141 gatggcgtga acaagccgaa actgtggttc tatctgccgg ttgattactg gcacaacgtc
    16201 gaaccgctgc cgacctcctt ctggactgaa gatgtggaag tgatcgcact gccgaaagcc
    16261 gatggcattg gtagcctgct gcccgctgcg cgcggcaata tcggttatat cggtccggtg
    16321 ccggaacgtg cgctgcaact gggtattgag gccagcaaca tcaacccgaa aggggtgatc
    16381 gactacctgc attactaccg ctccttcaaa accgagtacg aactggcctg tatgcgtgaa
    16441 gcgcagaaaa tggcggtcaa cggtcatcgt gcggcagaag aagcgttccg ttctggcatg
    16501 agcgagttcg atatcaacat cgcctatctg accgcgaccg gtcatcgtga taccgacgta
    16561 ccttacagca acattgtggc gcttaacgaa cacgcttcgg tgctgcatta caccaaactg
    16621 gatcatcagg caccggaaga gatgcgcagc ttcctgctgg atgccggggc cgaatataac
    16681 ggctatgcgg ctgacctgac tcgtacctgg tcggcaaaaa gcgacaacga ctacgcacag
    16741 ctggtgaaag acgtaaatga tgaacaactg gcgctgatcg ccaccatgaa agcaggcgtt
    16801 agctatgtgg attaccacat ccagttccat cagcgcatcg ccaaactgct gcgtaaacat
    16861 caaatcatca ccgatatgag tgaagaagcg atggtcgaaa acgatctcac cggaccgttt
    16921 atgccgcatg gtatcggtca tccgctgggc ctgcaggtgc atgacgtagc cggttttatg
    16981 caggatgata gcggtacaca cctcgcggca ccggcaaaat atccgtacct gcgctgcacc
    17041 cgtattctcc agccgggtat ggtgttaacc atcgaaccgg gtatctactt cattgaatcg
    17101 ctactggcac cgtggcgtga agggcagttc agcaagcact tcaactggca gaaaattgaa
    17161 gcactgaaac cgttcggcgg cattcgtatc gaagacaacg tggtgatcca cgaaaacaac
    17221 gtggaaaaca tgacccggga tctgaaactg gcgtgatgga aagctggtta attcctgcgg
    17281 caccggtcac ggtcgttgaa gagatcaaaa aaagccgctt cataacgatg ttggcgcata
    17341 ccgatggcgt tgaggcggcg aaagcgtttg ttgaatcggt gcgggcagaa caccccgatg
    17401 cccgccacca ttgcgtggcg tgggtggcgg gtgcgccgga tgattctcaa cagctgggtt
    17461 tctctgacga cggggagccg gcaggaacgg caggtaaacc gatgctcgcc cagctaatgg
    17521 gcagcggcgt cggggaaatt accgctgtgg tagtgcgcta ctacggcggc atattgcttg
    17581 gcaccggtgg gttagtgaaa gcgtatggcg gcggcgtgaa tcaggcgctg cgccagctaa
    17641 tgacccaacg caagacgcca ttaaccgaat atactttgca atgtgaatat catcagttaa
    17701 ccggcattga agcgttgctg gggcagtgtg acggcaaaat tatcaacagt gattatcagg
    17761 cattcgttct gctgcgggtg gcgcttccgg cggcgaaagt ggctgaattt tccgcaaagc
    17821 tggcggattt tagccgtggt tcattgcaat tgttagcgat tgaagaataa tccccacttc
    17881 gttttgcaga actaaggaag cggcagagat gcattttcgc gccattaccc gaatcgttgg
    17941 actactggtc atcttatttt cagggaccat gattatccct gggctggtag cactcatcta
    18001 ccgggatgga gcgggccgcg cttttaccca gacctttttt gtcgccctcg ccattggttc
    18061 tatgctgtgg tggccgaacc gtaaagagaa gggcgaactg aaatcccgtg aggggtttct
    18121 gatagtggtg ctgttctgga ccgtgctggg tagtgtcggt gcgctccctt ttatcttctc
    18181 ggaaagcccg aacctcacga ttaccgatgc gttttttgaa tctttctctg gcctgaccac
    18241 caccggggcc actacgctgg tggggctgga ttcgctccct catgccatcc tcttttatcg
    18301 ccagatgctg caatggtttg gcgggatggg gatcatcgtg ttagcggttg cgatactgcc
    18361 tatcctcggc gtgggtggga tgcagctcta tcgcgcagaa atgcccggcc cgctgaaaga
    18421 taacaaaatg cgcccgcgaa ttgcggaaac ggcgaaaacc ctgtggttga tttatgtctt
    18481 gctgaccgtc gcctgtgcgc tggcgttgtg gtttgccgga atggatgcct ttgatgccat
    18541 cggccatagc tttgcgacta tcgctattgg cggcttctcg acacatgatg ccagtatcgg
    18601 ttatttcgac agcccgacta ttaacactat cattgctatc ttcctgctga tctccggctg
    18661 taactacggt ctgcactttt cactgttaag tgggcgtagt ctgaaggttt attggcgcga
    18721 tccggaattt cgcatgttta tcggcgtaca gtttacgctg gtggttattt gtacactcgt
    18781 actgtggttt cataatgtct acagttcggc gctgatgaca attaaccagg cgtttttcca
    18841 ggtggtatcg atggcgacaa ccgccgggtt tacgactgac agcattgccc gctggccgct
    18901 ctttttgccg gtactgcttt tatgttcagc ttttatcggc ggttgcgccg ggtcaacggg
    18961 cggtggcctg aaagtgatcc gcatcctgct gctgtttaag caggggaacc gtgagctgaa
    19021 acgactggtg catccgaacg ccgtctatag cattaagctg gggaatcgcg cactgccgga
    19081 acgtatcctc gaagccgttt ggggattttt ctccgcctat gcattggtgt ttattgtcag
    19141 tatgctggcg attatcgcca cgggcgtgga tgacttttct gcctttgctt cagttgttgc
    19201 gacattgaat aacctggggc cagggcttgg cgtggttgct gataacttta ccagtatgaa
    19261 cccggtggct aaatggatcc tgattgccaa catgctgttt ggtcgtctcg aggtctttac
    19321 attgctggtg ctctttaccc cgactttctg gcgtgaatga tggagtaata cgtgaaaaca
    19381 ttaattcttt tctcaacaag ggacggacaa acgcgcgaga ttgcctccta cctggcttcg
    19441 gaactgaaag aactggggat ccagacggat gtcgccaatg tgcaccgcat tgaagaacca
    19501 caatgggaaa attatgaccg tgtggtcatt ggtgcttcta ttcgctatgg tcactaccac
    19561 tctgcgttcc aggaatttgt caaaaaacat gcgacgcgac tgaattcgat gccgagcgcc
    19621 ttttattccg taaacctggt ggcgcgcaaa ccggagaagc gtactccaca gaccaacagc
    19681 tacgcgcgga agtttctgat gaactcgcaa tggcgtcccg atcgctgcgc ggtcattgcc
    19741 ggggcgctgc gttacccacg ttatcgctgg tacgatcgtt ttatgatcaa gctaattatg
    19801 aagatgtcag gcggtgaaac ggatacgcgc aaagaagttg tctataccga ttgggagcag
    19861 gtggcgaatt tcgcccgaga aatcgcccag ttaaccaaca aaccgacgct gaaataagca
    19921 taaagaataa aaaatgcgcg gtcagaaaat tattttaaat ttcctcttgt caggccggaa
    19981 taactcccta taatgcgcca ccactgacac ggaacaacgg caaacacgcc gccgggtcag
    20041 cggggttctc ctgagaattc cgacagagaa agcaaaaata aatgcttgac tctgtagcgg
    20101 gaaagcgtat tatgcacacc ccgcgccgct gagaaaaagt gaagcggcac tgctctttaa
    20161 caatttatca gacaatctgt gtgggcactc gaagatacgg attcttaacg tcgcaagacg
    20221 aaaaatgaat accaagtctc aagagtggac acgtaattca
//
LOCUS       DAZPDU010000069        20152 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZPDU010000069 DAZPDU010000000
VERSION     DAZPDU010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20152
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.070"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            9..890
                     /gene="yieE"
                     /locus_tag="ACHURS_002248"
     CDS             9..890
                     /gene="yieE"
                     /locus_tag="ACHURS_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFR1237966.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            1095..2564
                     /gene="lysP"
                     /locus_tag="ACHURS_002249"
     CDS             1095..2564
                     /gene="lysP"
                     /locus_tag="ACHURS_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFR1237967.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            2856..4847
                     /gene="cirA"
                     /locus_tag="ACHURS_002250"
     CDS             2856..4847
                     /gene="cirA"
                     /locus_tag="ACHURS_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFR1237968.1"
                     /translation="MFRLNPFVRVGLCLSAISCVWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(4879..5715)
                     /gene="yeiG"
                     /locus_tag="ACHURS_002251"
     CDS             complement(4879..5715)
                     /gene="yeiG"
                     /locus_tag="ACHURS_002251"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFR1237969.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            5973..6641
                     /gene="folE"
                     /locus_tag="ACHURS_002252"
     CDS             5973..6641
                     /gene="folE"
                     /locus_tag="ACHURS_002252"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFR1237970.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            6658..7815
                     /gene="yeiB"
                     /locus_tag="ACHURS_002253"
     CDS             6658..7815
                     /gene="yeiB"
                     /locus_tag="ACHURS_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFR1237971.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRT
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            7999..8997
                     /gene="galS"
                     /locus_tag="ACHURS_002254"
     CDS             7999..8997
                     /gene="galS"
                     /locus_tag="ACHURS_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFR1237972.1"
                     /translation="MATVSRVLNNSTLVSADTREAVMKAVSELDYRPNANAQALATQV
                     SDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAEKERHAIEVLIRQRC
                     NALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDNLSGARMATRMLLNN
                     GHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGTPDMPGGEAAMVELL
                     GRNLQLTAIFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIPIARYTDPQLTTVRY
                     PIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQNAAAITNSTNQAM"
     gene            9277..10275
                     /gene="mglB"
                     /locus_tag="ACHURS_002255"
     CDS             9277..10275
                     /gene="mglB"
                     /locus_tag="ACHURS_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFR1237973.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            10336..11856
                     /gene="mglA"
                     /locus_tag="ACHURS_002256"
     CDS             10336..11856
                     /gene="mglA"
                     /locus_tag="ACHURS_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFR1237974.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKFRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            11872..12882
                     /gene="mglC"
                     /locus_tag="ACHURS_002257"
     CDS             11872..12882
                     /gene="mglC"
                     /locus_tag="ACHURS_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFR1237975.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(13009..14244)
                     /gene="preA"
                     /locus_tag="ACHURS_002258"
     CDS             complement(13009..14244)
                     /gene="preA"
                     /locus_tag="ACHURS_002258"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFR1237976.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(14238..15476)
                     /gene="preT"
                     /locus_tag="ACHURS_002259"
                     /pseudo
     CDS             complement(14238..15476)
                     /gene="preT"
                     /locus_tag="ACHURS_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
     gene            complement(15670..15909)
                     /locus_tag="ACHURS_002260"
     CDS             complement(15670..15909)
                     /locus_tag="ACHURS_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFR1237977.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(15912..16631)
                     /gene="sanA"
                     /locus_tag="ACHURS_002261"
     CDS             complement(15912..16631)
                     /gene="sanA"
                     /locus_tag="ACHURS_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFR1237978.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFRREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(16781..17665)
                     /gene="cdd"
                     /locus_tag="ACHURS_002262"
     CDS             complement(16781..17665)
                     /gene="cdd"
                     /locus_tag="ACHURS_002262"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFR1237979.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(17795..18490)
                     /locus_tag="ACHURS_002263"
     CDS             complement(17795..18490)
                     /locus_tag="ACHURS_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFR1237980.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(18487..18885)
                     /locus_tag="ACHURS_002264"
     CDS             complement(18487..18885)
                     /locus_tag="ACHURS_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFR1237981.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            19124..20071
                     /gene="dusC"
                     /locus_tag="ACHURS_002265"
     CDS             19124..20071
                     /gene="dusC"
                     /locus_tag="ACHURS_002265"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFR1237982.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKA
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
BASE COUNT         4935 a         4980 c         5271 g         4966 t
ORIGIN      
        1 aagcgactat gcacatcacc ctccggcagt tggaagtttt tgcagaagta ttgaaaagtg
       61 gatcaaccac ccaggcgtcg gtgatgctgg cgttgtcgca atcagcagtg agcgcagcct
      121 tgaccgacct ggaagggcag cttggcgtgc aactgtttga tcgcgtgggg aaaagactgg
      181 ttgttaatga acacgggcgg ctgctctatc cgcgtgcgtt ggcattgctt gaacaggcgg
      241 ttgaaatcga acaactgttt cgcgaagaca acggcgcgat tcgtatctat gccagtagta
      301 ccatcggtaa ctacattctg cctgcagtta tcgcccgtta tcgccatgat tatccgcagt
      361 tgccgattga acttagcgtt gggaacagcc aggacgtgat gcaagcggtg ctggatttcc
      421 gcgttgatat tggctttatt gaaggaccgt gccacagcac tgaaatcatt tctgaaccgt
      481 ggctggaaga cgagctggtg gttttcgccg cgccgacttc gccgttggct cgtggtccgg
      541 tcaccttaga acagctggcc gctgcgccgt ggatcctgcg tgaacgcggt tccggcacgc
      601 gggagattgt cgattatctg ttgctgtcac atttaccgaa gtttgagatg gcgatggaat
      661 taggtaactc cgaggcaatc aaacatgcgg tgcgtcatgg gttgggaatt agttgcctgt
      721 cgcgacgtgt gattgaagat caattgcagg caggcacatt aagtgaagtt gcggtccctc
      781 tcccgcgcct gatgcgtacg ttgtggcgta tacatcatcg gcaaaaacac ctttccaacg
      841 cgctacggcg ctttctggac tattgcgatc ccgcgaatgt accgcgttaa ggtgctgtac
      901 aagaacatgc tggtgctgcg tcgatttcgt gacgcagcgc cctcagcatg cattcgccag
      961 aaaagagatt ggctgcttta cttataatcc ctgggcgatc atgaaggtgt cttataaccg
     1021 tgtatttctg ccggaaggat tgccaatcgt ctgctacaat cgcgcctcat ttttaagatg
     1081 gatagcattt ttgtatgggt tccgaaacta aaactacaga agcgccgggc ttacgccgtg
     1141 aattaaaggc gcgtcacctg acgatgattg ccattggcgg ttccatcggt acaggtcttt
     1201 ttgttgcctc tggcgcaacg atttctcagg caggtccggg cggggcattg ctctcgtata
     1261 tgctgattgg cctgatggtt tacttcctga tgaccagtct cggtgaattg gctgcatata
     1321 tgccggtttc cggttcgttt gccacttacg gtcagaacta tgttgaagaa ggctttggct
     1381 tcgcgctggg ctggaactac tggtacaact gggcggtgac tattgccgtt gacttggttg
     1441 cagcacagct ggtcatgagc tggtggttcc cggatacacc gggctggatc tggagtgcgt
     1501 tgttcctcgg cgttatcttc ctgctgaact acatctcagt tcgtggcttt ggtgaagcgg
     1561 aatactggtt ttcactgatc aaagtcacga cagttattgt ctttatcatc gttggcgtgc
     1621 tgatgattat cggtatcttc aaaggcgcgc agcctgcggg ctggagcaac tggacaatcg
     1681 gcgaagcgcc gtttgctggt ggttttgcgg cgatgatcgg cgtagctatg attgtcggct
     1741 tctctttcca gggaaccgag ctgatcggta ttgctgcagg cgagtccgaa gatccggcga
     1801 aaaacattcc acgcgcggta cgtcaggtat tctggcgaat cctgttgttc tatgtgttcg
     1861 cgatcctgat tatcagcctg attattccgt ataccgatcc gagcctgctg cgtaacgatg
     1921 ttaaagacat cagcgtcagt ccgttcacct tggtgttcca gcacgcgggt ctgctctctg
     1981 cggcggcggt gatgaacgca gttattctga cggcggtgct gtcagcaggt aactccggta
     2041 tgtatgcgtc tactcgtatg ctgtacaccc tggcgtgtga cggtaaagcg ccgcgcattt
     2101 tcgctaaatt gtcgcgtggt ggcgtgccgc gtaatgcgct gtatgcgacg acggtgattg
     2161 ccggtttgtg cttcctgacc tccatgtttg gcaaccagac ggtatacctg tggctgctga
     2221 acacctccgg gatgacgggt tttatcgcct ggctggggat tgccattagc cactatcgct
     2281 tccgtcgcgg ttacgtattg cagggacacg acattaacga tctgccgtac cgttcaggtt
     2341 tcttcccact ggggccgatc ttcgcattca ttctgtgtct gattatcact ttgggccaga
     2401 actacgaagc gttcctgaaa gataccattg actggggcgg cgtagcggca acgtatattg
     2461 gtatcccgct gttcctgatt atttggttcg gctacaagct gattaaagga actcacttcg
     2521 tacgctacag cgaaatgaag ttcccgcaga acgataagaa ataagtttcc tcccttcctt
     2581 gctaagccct ctcaaccgag agggcttttt caattccatt tccctgacaa atcatgcgga
     2641 tataaaattt aacatttgga ttgataactg ttatcgtttg cattatcgtt acgctacaat
     2701 caaaaaaggc tgacaaatca gaggctggtc cggctttctg ggatgatcac ctgcatacaa
     2761 ataagtccac cgcgatgctg ccgtacgcaa ggggacgtaa agaagatgtg agcgataccc
     2821 attttatttt cgtagttacc tcatggagat atggaatgtt taggttgaac cctttcgtac
     2881 gggtcgggct gtgtttgtcc gctatttctt gtgtatggcc tgtgttagcg gtcgatgacg
     2941 atggcgaaac gatggttgtc actgcatctt ccgttgaaca aaaccttaaa gatgcacccg
     3001 ccagtatcag cgtcattacc caggaagatc tgcagcgaaa accggtacag aatctgaagg
     3061 atgtcctcaa agaagtgcct ggcgtacaac tgacgaacga aggggataac cgtaagggcg
     3121 ttagtattcg tggtctggac agcagctata ccctgattct cgtcgacggt aaacgcgtga
     3181 actcccgcaa tgccgtcttc cgccacaatg atttcgatct gaactggatc ccggtcgatt
     3241 ccatcgaacg tattgaagtg gtccgtggcc cgatgtcgtc gctgtacggt tccgatgcgc
     3301 tcggcggtgt agtgaatatc atcaccaaaa aaatcggtca gaaatggtcg ggtaccgtta
     3361 ccgtcgatac caccattcag gaacatcgcg atcgcggtga tacctataac ggtcagttct
     3421 ttaccagtgg accattaatt gatggtgtgc tgggaatgaa agcttacggc agcctggcaa
     3481 aacgtgaaaa ggatgacccg caaaactcaa cgaccaccga taccggagaa acgccgcgta
     3541 ttgaaggatt ctccagccgc gacggcaatg tcgaatttgc ctggacaccg aatcaaaatc
     3601 acgattttac tgccggatac ggtttcgacc gtcaggatcg tgattccgac tcgctggaca
     3661 aaaaccgcct ggaacgccag aactactccg tcagccataa tgggcgttgg gattacggca
     3721 ccagcgaact gaaatactac ggtgagaaag tcgagaacaa aaaccctggc aacagcagcc
     3781 cgataacttc cgaaagcaat acggtcgacg gcaaatacac gttgccgctg acggcgatta
     3841 atcagtttct cacggttggc ggtgaatggc gtcacgacaa acttagcgat gcggtgaacc
     3901 tgaccggggg aaccagctcc aaaacgtctg ccagccagta cgcgctgttt gtggaagatg
     3961 aatggcggat cttcgagccg ctggcgctga cgaccggcgt gcgtatggac gatcacgaaa
     4021 cctacggtga acactggagt ccgcgtgcct acctggttta taacgccacc gacaccgtaa
     4081 cggtgaaagg gggctgggcg acggcattta aagcaccttc tctgttgcaa cttagccctg
     4141 actggacgag caattcctgc cgtggcgcat gtaagattgt gggtagcccg gatctgaaac
     4201 cagaaaccag cgaaagttgg gagctggggc tttactacat gggtgaagaa ggctggctgg
     4261 aaggggttga atccagcgtt accgttttcc gtaacgatgt gaaagatcgt atcagcatca
     4321 gccgtacgtc tgacgtcaac gctgcaccgg gctaccaaaa ctttgttggt tttgagacgg
     4381 gcgctaacgg acggcgcata ccggtattta gctactacaa cgttaacaaa gctcgtattc
     4441 agggcgtgga aaccgaactg aaaattccgt tcaacgatga atggaaactg tcgatcaact
     4501 acacctacaa cgatggtcgt gatgtcagca acggcgaaaa caaaccgcta tccgatctgc
     4561 cgttccatac tgctaacggt acgctggact ggaaaccgct ggcgctggaa gactggtcat
     4621 tctatgtttc tggtcactat accgggcaga aacgcgccga cagcgcgacg gctaaaacac
     4681 cgggcggtta taccatctgg aataccggcg cggcctggca ggtgactaaa gacgtcaaac
     4741 tgcgcgcagg cgtgctgaac cttggcgaca aggatctcag tcgtgacgac tacagctata
     4801 acgaagacgg acgtcgttac tttatggcag tggattatcg cttctgatga gaagatgccc
     4861 ggcgaaccgg gcggactttc acttcagtaa atactgcgca tggaagcgca ggtgatcctc
     4921 tataaaagag gcgatgaagt agtaactgtg atcatatccc ggctgaatac gcagcgtcat
     4981 cggccacgct ttctggcgcg cggcttctgc caataccgca ggttgcaact ggtcggcaag
     5041 aaactgatca ttatcgccct gatcgataag cgtcgggatc gcatcctgcg cgttactggc
     5101 atacatcagc gcgcaactat cccattccag ccatgcattt ttgtcctcac ctaaataggt
     5161 gctaaacgct ttgattcccc acgggacgct gcacggattc acaattggcg caaaggccga
     5221 aacgctggtg tatttacccg gatttttcag cgccataatc agcgcaccgt gaccacccat
     5281 tgagtgaccg ctaatggcgc agcggtcgct gacattaaat tgcgactgaa ccagcgccgg
     5341 taattcatcg cgcagataat catacatccg gtaatgcgtc gcccacggcg gttgcgtggc
     5401 attaagataa aagcctgcgc cctggcctaa atcgtagcca tcgtcgttgg caaccttttc
     5461 gccgcgcggg ctggtgtctg gcatcaccag tacaatcccc agttctgccg ccacgcgctg
     5521 ggcacccgcc ttggtggtga agttctcgtc attgcaggtt aatccggaaa gccagtacag
     5581 cactggtggc ggggtgtgat cacgaggggg tgggagaaag atactgaacg tcatcgggca
     5641 gtttaaggta ctggagtcgt gtcgccagcg ttgctgccag ccttcaaaac agcggtgctc
     5701 ttcgagcatt tccatgcgtg gctccttgtt gtgttgtttg caatttcttc atcataccgt
     5761 ttattcaatg tgctgtgagt aactttcact tccgtatttg cataacgatg ttttaacatc
     5821 tgctgatgaa agtcagcggc aattacaata attatcgctg tgaatactgg attatgtgcg
     5881 ccgcctcacg cacaataatc aggctgtaaa tcagcttaat aactttgccc ccacgcaggg
     5941 cggaggcgtc acacctgcag gagaaatcat aaatgccatc actcagtaaa gaagcggccc
     6001 tggttcatga agcgttagtt gcgcgaggac tggaaacacc gctgcgcccg cccgtgcatg
     6061 aaatggataa cgaaacgcgc aaaagcctta ttgctggtca tatgaccgaa atcatgcagc
     6121 tgctgaatct cgacctggct gatgacagtt tgatggaaac gccgcatcgc atcgctaaaa
     6181 tgtatgtcga tgaaattttc tccggtctgg attacgccaa cttcccgaaa atcaccctca
     6241 ttgaaaacaa aatgaaggtc gatgaaatgg tcaccgtgcg cgatatcact ctgaccagta
     6301 cctgtgaaca ccattttgtt accatcgatg gcaaagcgac ggtggcttat atcccgaaag
     6361 attcggtgat cggtctgtca aaaattaacc gtatcgtgca gttctttgcc cagcgtccgc
     6421 aggtgcagga acgtctgacg cagcaaattc ttattgcgct acaaacgctg ctgggcacca
     6481 ataacgtggc tgtctccatt gatgcggtgc attactgcgt gaaagcgcgt ggcatccgcg
     6541 atgcaaccag tgccacaaca acgacctctc ttggtggatt gttcaaatcc agtcagaata
     6601 cgcgccacga gtttctgcgc gctgtgcgtc atcacaactg attaaaaggc aggaaccatg
     6661 gagcgcaacg taacgctcga ttttgttcgc ggcgtcgcca ttctggggat cctgctatta
     6721 aacatcagcg cctttgggct accaaaggcg gcttatctca atcccgcctg gtacggcgct
     6781 attacgccgc aggatgcatg gacctgggca tttctcgatc tcatcggcca ggtgaaattc
     6841 ctcacgcttt ttgcgctgct gtttggtgcg ggcctgcaaa tgttgctgcc ccgtggcaga
     6901 cgctggatcc agtcgcggtt aacgctgtta gtcttgctgg gttttattca cggtttattg
     6961 ttctgggacg gcgatattct gctggcttac gggctggtgg gcttaatctg ctggcggctg
     7021 gtgcgcgatg cgccatcggt aaaaagcctg tttaatacag gcgtcatgct ttatctggtg
     7081 gggcttggcg ttttgctgtt attggggctg atttccgaca gccagaccag ccgcacctgg
     7141 acgccggatg catcggctat tttgtatgaa aaatactgga agcttcacgg cggcgttgaa
     7201 gcgatcagta atcgtgccga tggtgttggc aacagtttac tggcactggg cgcacagtat
     7261 ggctggcaac tggcagggat gatgctcatt ggtgcggcgc tgatgcgcag cggctggctg
     7321 aaagggcagt tcagcttacg tcactatcgt cgtactggtt ttgtgctggt ggcgattggg
     7381 gtgaccatta accttcctgc catcgccctg caatggcagc tggactgggc gtatcgctgg
     7441 tgtgccttct tacttcaaat gccgcgggaa ctgagtgcgc cgtttcaggc gattggctat
     7501 gcgtcgctgt tttatggctt ctggccgcaa ttgagccgct ttaagctggt gcttgcgatc
     7561 gcctgcgtcg gacggatggc gctgaccaac tatctattgc aaacgctgat ttgtaccatg
     7621 cttttttacc acctcggttt gtttatgcat tttgaccgcc tggagctgct ggcgtttgtt
     7681 attccggtat ggctggcgaa tatcctcttc tctgttatct ggctgcgtta cttccgccag
     7741 gggccggtgg aatggctctg gcgtcagtta actttgcgtg ctgccggaac ggcaatatct
     7801 aaaacatcaa gataacgatc tggatcacat tcgttaacaa aacggctgta accgtttcca
     7861 ttgctgtgac tcgattcacg aagtcctgta ttcagtgctg acaaaatagc cgccagcaag
     7921 cagtcattta ctgcaatctc ataacaggta gtgaatatga tcaccattcg tgatgtagcg
     7981 cgttaggctg gcgtctctgt ggcaacggtt tcccgggtgc tcaataacag cacgctggtc
     8041 agtgccgaca cgcgtgaagc agtaatgaaa gccgtgagtg agctggatta tcggccaaac
     8101 gccaatgccc aggcgctggc aactcaggtt agcgacacca ttggcgtggt ggtgatggac
     8161 gtttctgatg cgtttttcgg cgcgctggta aaagcggtgg atctagtcgc tcagcagcat
     8221 cagaaatacg tgctaatcgg caatagctat catgaagcgg aaaaagagcg tcacgccatt
     8281 gaggtgttaa ttcgccagcg ttgtaatgcg ttgattgttc actcaaaagc attgagtgac
     8341 gatgaactgg cgcaatttat ggataacatt cccggtatgg tgttaatcaa ccgcgttgtg
     8401 ccggggtacg cccatcgttg cgtttgcctg gataatctca gcggtgcccg aatggcgacg
     8461 cgcatgttgc tgaataacgg tcatcaacgt attggttatc tttcttccag tcacggcatt
     8521 gaagatgacg ccatgcgtaa agcaggctgg atgagtgcgt tgaaagagca ggatattatt
     8581 ccgccggaaa gctggattgg cactggtacg ccggacatgc cgggcggtga ggcggcgatg
     8641 gttgaactgc tggggcgcaa tctacaactt accgctatat ttgcttataa cgacaatatg
     8701 gccgctggcg cactgacagc attaaaagat aatggcattg cgattccgtt acatctctca
     8761 atcatcggtt tcgatgatat tcccatcgcc cgttataccg acccgcaatt aacgaccgtg
     8821 cgttatccca ttgcttcaat ggctaaatta gccaccgaac tggccttgca gggggcagca
     8881 ggcaatattg atcctcgtgc cagccactgt tttatgccga cgttagtgcg tcgccattct
     8941 gtcgcaacgc gccagaatgc ggcggcgatc actaactcaa caaatcaggc gatgtaaccg
     9001 ctttcaatct gtgagtgatt tcacagtatc ttaacaatgt gatagctatg attgcaccgt
     9061 tttaacgttg taacccgtat gtaacagtga ataatcactt tcaccgaggt aatagcgtca
     9121 taacaacaat taaagccgtt ttctggagcg ttaccgggca tggaagaacg aatttaaaaa
     9181 agtgagcttc ggcgttcagt aacacttcat taactctact gccccgccga gcatttatct
     9241 caagcactac cctgcataag aaaaaccgga gataccatga ataagaaggt gttaaccctg
     9301 tctgctgtga tggccagcat gttattcggt gccgctgcac acgctgctga tactcgcatt
     9361 ggtgtaacaa tctataagta cgacgataac tttatgtctg tagtgcgcaa ggctattgag
     9421 caagatgcga aagccgcgcc agatgttcag ctgctgatga atgattctca gaatgaccag
     9481 tccaagcaga acgatcagat cgacgtattg ctggcgaaag gggtgaaggc actggcaatc
     9541 aacctggttg acccggcagc tgcgggtacg gtgattgaga aagcgcgtgg gcaaaacgtg
     9601 ccggtggttt tcttcaacaa agaaccgtct cgtaaggcgc tggatagcta cgacaaagcc
     9661 tactacgttg gcactgactc caaagagtcc ggcattattc agggcgattt gattgctaaa
     9721 cactgggcgg cgaatcaggg ttgggatctg aacaaagacg gtcagattca gttcgtactg
     9781 ctgaaaggtg aaccgggcca tccggatgca gaagcacgta ccacttacgt gattaaagaa
     9841 ttgaacgata aaggcatcaa aactgaacag ttacagttag ataccgcaat gtgggacacc
     9901 gctcaggcga aagataagat ggacgcctgg ctgtctggcc cgaacgccaa caaaatcgaa
     9961 gtggttatcg ccaacaacga tgcgatggca atgggcgcgg tagaagcact gaaagcacac
    10021 aacaagtcca gcattccggt gtttggcgtc gatgcgctgc cagaagcgct ggcgctggtg
    10081 aaatccggtg cactggcggg cacagtactg aacgatgcta acaaccaggc gaaagcgacc
    10141 tttgatctgg cgaaaaacct ggccgatggc aaaggtgcgg ctgatggcac caactggaaa
    10201 atcgacaaca aagtggtccg cgtaccttat gttggcgtag ataaagacaa cctggctgaa
    10261 ttcagcaaga aataagactg attgggcgca gtcgtttact gcgcccttat tgtatggcca
    10321 acaaggtaaa attatatggt cagctcaacg actccgtctt ccggggaata cttgttggaa
    10381 atgagcggta tcaacaagtc ctttcctggt gttaaggcac ttgataacgt taatttaaaa
    10441 ttccggccac attctatcca tgcattaatg ggggaaaacg gtgcaggaaa atcgacatta
    10501 ttaaaatgcc tgtttggtat ttatcaaaaa gactccggca ccattttatt ccagggtaaa
    10561 gagatcgatt tccattccgc caaagaggca ctggaaaatg gtatttcgat ggtacaccag
    10621 gagttaaacc tggtattaca acgttcggtg atggataaca tgtggctggg gcgatatccc
    10681 accaaaggca tgtttgtcga tcaggacaaa atgtaccgcg aaaccaaagc gatttttgat
    10741 gaactggata ttgatatcga tccgcgtgcg cgcgtcggca cattatccgt ttcgcaaatg
    10801 cagatgatcg aaatcgccaa agcgttttcc tataacgcga aaattgtgat tatggatgaa
    10861 ccgacttctt cgttaaccga aaaagaggtc aatcatctgt tcactattat tcgtaaatta
    10921 aaagagcgcg gctgcggtat tgtttatatc tcgcataaaa tggaagaaat cttccagtta
    10981 tgtgatgaag ttaccgtatt gcgcgacggt cagtggatcg ccaccgaacc gctggcagga
    11041 ctgacgatgg acaagatcat cgccatgatg gttgggcgtt ctcttaatca gcgttttcct
    11101 gacaaagaaa acaagccggg cgaagtcatc ctcgaggtgc gtaacctaac gtcactgcgc
    11161 cagccgtcga ttcgcgatgt ctcgtttgat ctgcataaag gggagatcct cggtattgcc
    11221 gggctggtgg gggcgaaacg taccgatatt gttgagacgt tatttggtat tcgcgagaaa
    11281 tcggctggca ccattacgtt gcacggcaaa cagatcaata accataatgc caacgaagcc
    11341 ataaaccacg gatttgcact ggtaactgag gagcgccgct caacgggaat ttatgcctat
    11401 ctggatattg gttttaactc gttaatttcc aatattcgca actacaaaaa taaagttggt
    11461 ttactggata actcgcggat gaaaagcgat acccagtggg tgattgattc gatgcgggta
    11521 aaaacgccgg gtcatcggac gcaaattggt tcgctctccg gtggtaatca gcaaaaggtg
    11581 attattggtc gctggctact aacgcaacca gaaatattaa tgctcgatga accgacgcgc
    11641 ggtattgatg tcggggcgaa gtttgaaatt tatcagttaa ttgccgaact ggcgaagaaa
    11701 ggcaagggga ttattattat ctcctctgaa atgcctgagt tgttagggat aacagaccgt
    11761 attctggtca tgagcaatgg tctcgtttcc ggaattgtcg atacaaaaac aacaacgcaa
    11821 aacgaaattc tgcgtcttgc gtctttgcac ctttaagatt aggggcttcc catgagtgcg
    11881 ttaaataaga aaagttttct tacttacctg aaagagggcg gtatttacgt cgttctttta
    11941 gttttgctgg cgattattat tttccaggac ccaacatttt taagtctgtt gaacttaagt
    12001 aatattctca cccagtcatc ggtgcgtatt attatcgcgc tcggtgtggc agggttaatt
    12061 gtcacccagg ggaccgatct ttctgctggt cgtcaggtag ggctggcggc agtggtggct
    12121 gcgacattat tgcagtccat ggataacgcc aacaaagtgt tcccggaaat ggcgacgatg
    12181 ccgattgcgc tggttattct gattgtctgt gccattggtg cggtgatcgg tttgatcaac
    12241 ggtctgatta tcgcttatct caacgtgacg ccgttcatta ccacgctcgg cacgatgatc
    12301 atcgtctatg gcatcaactc gctctattac gactttgtcg gggcgtcgcc aatttctggt
    12361 tttgacagtg gcttctctac ctttgctcag ggctttgtcg cgctggggag tttccgtctc
    12421 tcttacatca ccttctacgc gttgattgcg gtggcgttcg tctgggtgtt gtggaacaaa
    12481 acccgcttcg gtaagaacat ttttgccatt ggcggtaacc cggaagcggc aaaagtatct
    12541 ggtgtcaacg tcggcctgaa cctgctgatg atctacgcgt tgtctggcgt gttctatgcc
    12601 tttggcggga tgttagaagc cggacgtatc ggctctgcca ccaacaacct cggctttatg
    12661 tatgagctgg atgctatcgc ggcgtgcgtg gtaggcggtg tatcgttcag cggcggtgtg
    12721 gggacggtga ttggcgtggt gaccggggtg attattttta ccgtcatcaa ctatggcctg
    12781 acgtatatcg gcgtaaaccc atactggcag tacatcatca aaggggcgat tattatcttc
    12841 gccgtagcgc tggattcact gaaatacgcg cgtaagaaat gatgtgaatg tggtccgcat
    12901 tcggtcataa acgcacataa catcatgatc tgtgtggttt tgtaggccgg ataaggcgtt
    12961 cacgccgcat ccggcaacag gaaccaaatg ccggatgtga cgcgggttct acaacgttac
    13021 cgggtgttct ttctcgcctt tcttaaactt cacttccccg agctcaatac aacccaccgg
    13081 gcagacgtga ccacaaagca gacaacccac acatttctcg gtattacaat gcggtgtgcg
    13141 ggttttctcg ctccattcca tcgcctggtg accgccgtcg taacaggaaa tataacagcg
    13201 tccacagcca acacatttgt caagattgat acggggatag acaatataac tgcggtctaa
    13261 atcttccgcc gggacaatat tgttattcgc cagacctacc atctcctgca acgaatcaaa
    13321 tccctgatcg gcgagataat gcgataaccc gctcgccata tcttctacga tgcgatagcc
    13381 gtactgcata atgccggtgg tgacctgtaa cgttgctgcg ccgagcaata aaaactccgc
    13441 cgcatcctcc caggtttcaa tgccgccgat accgctgatt gggaaatcgc gcagttctgg
    13501 atgggtgcgc atttgctgaa tgaagcgcag ggcgatcggt tttaccgctt taccggaata
    13561 tccggaaata cttgattttc cgttaacgat cggcataccg attttctgat taagatcgat
    13621 attggtgatg gatttaacgg tgttaatcgc cgcaatgcca tctgcgccgc cgcgcttcgc
    13681 cgccagcgcc acttcgcaca tatcaccgat attcggcgtc atcttcgcca gcattggcag
    13741 cgtggaaccc cgtttcactg cccgacaata tttttctacc agctccgggc tttgcccgac
    13801 atcgctaccc atcgcatgag aagtcatttg cggacaggag aagttacact cgatcatatc
    13861 cgcgccagct tcttgcacca ggcgcgccag ctcctcccat tgctgctcat tttcccccat
    13921 gatcgaagcg atcaatactt tgtccgggta atcttctttc agccgacgca gggcggccag
    13981 attctcctcc agcggatgct cggcaatctg ctccatattt ttgaagccga taaaaccggt
    14041 atcttctttc accagatgat caaaacgcgg cgagacttcg ttggcgataa aaaagccgat
    14101 cgttttaaac accacaccgc cccaacctgt gtcgtaggct ttggcacaca tctcatagca
    14161 gttgcctacc ggcgaagaag agaggcagaa cgggttggga aacttcacgc cgcaaaaagt
    14221 aatcgaaaga tctttcgtta acatgagcaa gctccctcta aatagtgatg aatcgcctcg
    14281 gcggcttctt tcccggtttt cacggcatag accacggttt tgtctccctc aacaatatcg
    14341 ccagcagcaa agacttgcgg gtcgcgggtc tggtaatttt gtgttttgat ggtgttacgc
    14401 tgcggctcta actccgcaaa ggcatctagt ctggcatgct gaccgacggc gagaataatt
    14461 ttatctgccg ccatcgtcag ttcgcccgat aaccgtacat gcttaaacgt gactttattg
    14521 ccttccacgg ctactggcgt gaatccatcg atgatcgaaa cgcccagctc ccgggcgctg
    14581 gtaaactctt tttcgcttgc cggaaactca tctaactctt cacgcgctac gcaagttacc
    14641 gcctgacagc cgagaacttt cagcgtgctg gctacgtcca tcgcgacatc accgccgccg
    14701 ataattaatg cgctttgtgg aatgctgata tcgccttgcg cctgtcgtgc acgttgcaag
    14761 aagtcgacgg caatctcaac gtcactatgc tcaaacagcg gtagcccgga accgcttgat
    14821 aaccccacgg tgaccagtac cgcgcggttt tctgctttca gctgctcaag ggtgagtgtg
    14881 ttaccgactt cgttgttgca cttaatggtc acgcccattt tttcaatacg ggcgatctct
    14941 gcatccagta ctgattgcgg taaccggaat tgcggaatgc cgttacgcaa tcagccaccg
    15001 ggttgtgctt ctttctcata gatcgtgacg tcataaccct ggtttgtcag tgtcacactg
    15061 gcctgcaatc cggcaggacc tgcgccaata atcgcgactt tgccgagcgt tttagtaccg
    15121 ggctgataaa tttccatccc ggtttgttgt tcaaaatcag taacaaaacg ctgtaagcgg
    15181 ccgatatcaa tgggagcatc aacaccggca cgggtacaac cgctttggca taatttctcc
    15241 gtcgggcaca ctctggcaca aacggcaccg agggcattat tttcgcgaat tgtctcggca
    15301 gcgcctttaa aattacgaaa gtagattgag cgaataaatt tccccggatc ggtctgtgcc
    15361 gggcaagcct gactacaggg agcgtcatga cataataaac agcgagaggc ttctttaatc
    15421 gccagtaaag gcgtaaaagc cggagtgagt tcatccagat aattttgttg cggcatgtga
    15481 tcttccttaa tgctatgagt aattaaggcg aacgctaaat aaatattgcg tgctgatatt
    15541 aaggctaagc gtgtggggaa atttaaactg tgaaaggatt cacaaatagc tgcggttcag
    15601 aatgttatat ccgcagctta ttgagtgaag taataattat tctcaataat taacagaaaa
    15661 gagaaaatat taacgataga aaataagata aacaatcatg ccgatagata aaattccgaa
    15721 gccgatatat gccaccatat aagcgaaaaa gagtccggga ttttttgctc gccagacata
    15781 caccggttcc ggtgcatttt taacccgttt atcaatcgcg cctgcgcgcc agcctttcca
    15841 tgcttcgcgg aaacaaaata tcgggataag gaaaaaaacg gcaacgacaa aaaactgctg
    15901 tacatccata actactttcc ttgtttcttt tgtaattcaa gtaactgttc gggtgtgacg
    15961 gcgggatacc cctgcgcatc ttccggcacc tggtgcatag ccggaatagg gaccagcggc
    16021 cctaaaaaac gcggttcacg tctaaaaata taaaggtcag ccagcgcacc gaaacgggcg
    16081 gcaaattcgc gaatacgtac tgacagcata tctttcggtg acggtacggc ataacactga
    16141 gcctgaatcc ccatatgcag cgcaataaat aatgcccgct cacagtggaa acgttgggtg
    16201 ataataatga aatcattagt atcgaacact ttgcgtgtac gcacgatgga atccagcgta
    16261 cgaaagcctg cgtaatcgag aacaatatct gatgggtcga caccagcagc gattaaatct
    16321 ttgcgcatgg tcatcggctc attataactt tgcaatgcgt tatcgccgct cagtaataga
    16381 taatttacct taccgctgtt ataggcatta atcgctcctt gaatgcggta gcgataatac
    16441 tgattaatta cgccagtacg ataatatttt gctgtgccga gcaccacgcc gacctggcgg
    16501 taggggagat cctgcaattc gtcgtagata taaggcgcgg ttttccagct catccagcga
    16561 tcgaggccga gcacagtcaa cagcagcaag ccgatcagga ctaacaggct gaggaacacg
    16621 cgctttaaca tgcaggagac tcgatctaag gaaaatttcc atcaggctac tttacccgct
    16681 ggttttgcgc aagaaacggc aaactgtttg ccgggaattt cgacaccagg agagggctgg
    16741 caagccggac aaggcgttca cgccgcatcc ggcaccaggc ttaagcgaga agcacgcggt
    16801 cgatactgtg acagccgaga gctttcagcg ttgcggaggt ggcatcccac tgaatcaacg
    16861 gcgcatcggc tttttctgcc agaaccgcgc gctggatatc cgggtaatca taacccttga
    16921 gattcaacag aattaacgcc ccctgcaacg gtggcagagt cgggttgaat gcggcgtttt
    16981 cagcgtagct gccgctgaaa atacgaccgt ctttacattc cagcgcgaca ccgcttggcg
    17041 acttactgta aggcatgtgc gaacggtttg ccgccgcaat cgctgcctga gaaagcgcat
    17101 cacccgtcag cgcatagccg tgatcctgtt cgtccatcag cagcgtttta atctccagat
    17161 ctttcggccc aaaggcatcc ggcagatagt cacgcagcgc gtgtgcctcg cggcccggca
    17221 gatgaatacg cagatccaga ccgctgttca gttcattcat aaactgacgg cagtgaccac
    17281 aaggcgtata gttaacggtg atggctgcaa gcgctttttc accactcaac caggcgtggc
    17341 tgatcgcgct ttgttcggca tgaacggttt gctgcattgt tgcaccaata aattccatat
    17401 tggcaccgaa ataccaggtt ccgctcacac cgcgcgcaat tgcgccaaca ttaaaattcg
    17461 acaatggcgt acgcgcacag gccgccgcca gtggaagtag tgcgaatgcc agcgcgtctt
    17521 cgtccagccc cgttgcgctc ttcagcgatg agacttgctc cccggtcaac aaagcgggga
    17581 agtacttgtc tgccagaata ggttccagtg cagattgcaa gttatccgca agttgggcaa
    17641 aagcggtttg aaaacgtgga tgcatggcgt tgcctcataa tcatgtaatg ggataacagt
    17701 ttacgaaccc gttatggctt tatatgtgat ctgaatctca ttaattatgc aacgcgtgaa
    17761 acttacatca aaaatgcgcg acgcatcgca aattttagcc cattactgcc agaataatcg
    17821 ggaaaaggaa cggtgcgatc agcgaagtaa ttatcccgca taacaccagc gccagcgaac
    17881 taaatgcacc ttcctgataa tccagctcgg cgcagcgcgc cgtaccgagg gcgtgcgagg
    17941 ctgtccccat tgccagaccg cgcgcagctt tggtacgaat acgcatcgca ttaagcaatg
    18001 tatggccaaa taccgcgccg aggatgccga cgaaaatcac gcaaactgcg ctgattgccg
    18061 gaataccgcc aatactgccg ccaaccgcca ttgcaatagg cgtggtgact gatttcggca
    18121 ggattgacgc ggcgatttcc ggtgaagcgc ccatcaacaa tgccacggaa gtccccgtca
    18181 ccatcgcaac cacgctgccg ataaaacaga tggtgatgat cgatttccag cgcgcgcgga
    18241 tctggtgtag ctgctcatat aaaggatagg ccagcgcgac gaccgccggt tgcagcagat
    18301 cgtttagcac ttcgctacct ttaaagtagc tgtcgtaaga gatgccggtc agcattaaaa
    18361 aaggaatgat caccaccatt gctaccagca acgggttaag caggggaaac ttataccgtg
    18421 ccgccagttt gcgggcggca aaaaagacaa tcaacgtcag cggtaatgac caccagatat
    18481 tcgccatcat tcttctgatc ctttctgacc tacgacttta cgttcaccgt gtaccagttg
    18541 tgaactccag ctcaccacca gaaaaaccac cagcgtactg actgcacagg aaaccactac
    18601 cgggccaaac tgtgcgcgga gcaaatcaaa atattgcatg acgcctacgc caatcggcac
    18661 aaacaatagc gccatatagc gaatcagtac gtagcacccc ggattgaccc attttgccgg
    18721 aagaatttgc aaggccagca ggacaaacag gatcagcatc ccgatgatgc tgcccggaat
    18781 ggttaccggt agcagggaag caatgaaaat gcctgcatac aggcaggcat aaatcaggac
    18841 gaaagcgcgt aaatattgcc agataatgtt cagtgtctta ctcataatca acaccccaga
    18901 tgagatggat tcatcataca attaattcgt aaaacgtgct accgatcaca ttaagaaatc
    18961 agcgcttatt ttttcgctcc ccggcattac gcaatgcgca acgaactcag gcatacatca
    19021 agcatagaga gggattacag tttgcagcaa tccctctttg cagaccttta atccgttgac
    19081 cataggcgct aacatagcgc ctcatttttt tccgggtgaa gatatgcgtg tgttactggc
    19141 tccgatggag ggtgtgcttg actctctggt gcgagaattg ctgaccgaag ttaacgacta
    19201 cgatctgtgc atcaccgagt ttgtccgcgt ggtggatcaa ctgctgccgg taaaagtctt
    19261 tcatcgcatt tgccctgagc tacaaaacgc cagccggaca ccatccggta cgctggtgcg
    19321 cgttcagttg ttaggtcagt tcccacaatg gctggcagag aacgccgccc gtgcggtgga
    19381 attaggttcc tggggcgtgg atctcaattg cggctgcccg tcgaaagcgg ttaacggcag
    19441 cggcggcggg gcgacgttac tcaaagatcc tgaactcatc taccagggtg caaaagcgat
    19501 gcgtgaagct gtaccggcgc atttgcccgt cagcgtgaaa gtgcgtctgg gctgggacag
    19561 cggtgagaag aaatttgaaa tcgccgatgc ggttcaacag gctggtgcta cggagctggt
    19621 ggtgcatgga cggacaaaag agcagggtta ccgcgcggag catattgact ggcaggcgat
    19681 tggcgagatt cgccagcggc tgaatattcc ggtgattgcc aacggcgaaa tctgggactg
    19741 gcagagcgcg caacaatgca ttgcgatcag cggctgcgac gcagtgatga ttggtcgcgg
    19801 ggcgctcaat attcccaacc tgagccgggt agtaaaatat aacgaaccgc gaatgccgtg
    19861 gccggaggtg gttgctttgc tgcaaaaata tacccgtctg gaaaagcagg gcgataccgg
    19921 gttatatcac gttgcgcgga ttaaacagtg gttgagttat ttgcgtaaag aatacgatga
    19981 agcaacggaa ttatttcagc atgttcgggt gttgaataat tcccctgata ttgcaagggc
    20041 tattcaggca attgatatcg agaaactcta aaaaaccata gtgatgtttt ttgacaatgt
    20101 gttagctctt tatcaatagg ccgcacggtt gtttggtggc gtcaaaattg cc
//
LOCUS       DAZPDU010000070        20108 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZPDU010000070 DAZPDU010000000
VERSION     DAZPDU010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20108
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.071"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            181..852
                     /locus_tag="ACHURS_002266"
     CDS             181..852
                     /locus_tag="ACHURS_002266"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HFR1237983.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            862..2058
                     /locus_tag="ACHURS_002267"
     CDS             862..2058
                     /locus_tag="ACHURS_002267"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFR1237984.1"
                     /translation="MKTWIFICMSIAMLLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIKRGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVIYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWIGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            2058..2618
                     /locus_tag="ACHURS_002268"
     CDS             2058..2618
                     /locus_tag="ACHURS_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFR1237985.1"
                     /translation="MLKRKIIQIADQVIIDETAGEVVIGANTRICHGAVIQGPVVIGA
                     NCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCYIADSVVANQAYLGA
                     QVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRIISPNTQL
                     GPRVIVERNLPTGTYSLRQELIRTGD"
     gene            2620..3393
                     /locus_tag="ACHURS_002269"
     CDS             2620..3393
                     /locus_tag="ACHURS_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HFR1237986.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3581..3856
                     /gene="frmR"
                     /locus_tag="ACHURS_002270"
     CDS             3581..3856
                     /gene="frmR"
                     /locus_tag="ACHURS_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFR1237987.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            3891..5000
                     /gene="frmA"
                     /locus_tag="ACHURS_002271"
     CDS             3891..5000
                     /gene="frmA"
                     /locus_tag="ACHURS_002271"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFR1237988.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5093..5926
                     /gene="fghA"
                     /locus_tag="ACHURS_002272"
     CDS             5093..5926
                     /gene="fghA"
                     /locus_tag="ACHURS_002272"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFR1237989.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6152..6691)
                     /locus_tag="ACHURS_002273"
     CDS             complement(6152..6691)
                     /locus_tag="ACHURS_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFR1237990.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYTIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(6793..8004)
                     /gene="mhpT"
                     /locus_tag="ACHURS_002274"
     CDS             complement(6793..8004)
                     /gene="mhpT"
                     /locus_tag="ACHURS_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFR1237991.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8291..9304)
                     /gene="mhpE"
                     /locus_tag="ACHURS_002275"
     CDS             complement(8291..9304)
                     /gene="mhpE"
                     /locus_tag="ACHURS_002275"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFR1237992.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVRHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9301..10251)
                     /gene="mhpF"
                     /locus_tag="ACHURS_002276"
     CDS             complement(9301..10251)
                     /gene="mhpF"
                     /locus_tag="ACHURS_002276"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFR1237993.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(10248..11057)
                     /gene="mhpD"
                     /locus_tag="ACHURS_002277"
     CDS             complement(10248..11057)
                     /gene="mhpD"
                     /locus_tag="ACHURS_002277"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFR1237994.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDHAEAAYAIQHI
                     NVQYDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(11067..11933)
                     /gene="mhpC"
                     /locus_tag="ACHURS_002278"
     CDS             complement(11067..11933)
                     /gene="mhpC"
                     /locus_tag="ACHURS_002278"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFR1237995.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(11951..12895)
                     /gene="mhpB"
                     /locus_tag="ACHURS_002279"
     CDS             complement(11951..12895)
                     /gene="mhpB"
                     /locus_tag="ACHURS_002279"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFR1237996.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(12897..14561)
                     /locus_tag="ACHURS_002280"
     CDS             complement(12897..14561)
                     /locus_tag="ACHURS_002280"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFR1237997.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     QRETIKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLHVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            14603..15436
                     /locus_tag="ACHURS_002281"
                     /pseudo
     CDS             14603..15436
                     /locus_tag="ACHURS_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
     gene            15513..16595
                     /gene="lacI"
                     /locus_tag="ACHURS_002282"
     CDS             15513..16595
                     /gene="lacI"
                     /locus_tag="ACHURS_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFR1237998.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            16718..19792
                     /gene="lacZ"
                     /locus_tag="ACHURS_002283"
     CDS             16718..19792
                     /gene="lacZ"
                     /locus_tag="ACHURS_002283"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFR1237999.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPAENPTGCYSLTFNIDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGKNRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PSTQISDFHVATHFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLRLNVENPALWSAEIPNLYRAVVELHTDDGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRFGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWSVALDGKPLASGEMPLDVAPQDKQLIE
                     LPELPQPESAGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAAPHAIPQ
                     LTTSETDFCIELGNKRWQFNRQSGFLSQMWIGAEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGSYHYQLVWCQK"
     gene            19844..>20108
                     /locus_tag="ACHURS_002284"
     CDS             19844..>20108
                     /locus_tag="ACHURS_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1238000.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFA"
BASE COUNT         4725 a         5513 c         5224 g         4646 t
ORIGIN      
        1 acttaacata tgaattgcat aaaaacatta cccagtcgaa cctccgggtc tgaaagccct
       61 tgcttttcag ttattagcaa catttaacat ttataaatta acatctggct gcgccagaga
      121 ttacgtctct gagtgcatcg ttgtgcttgt agtataataa ataaaatgga gtgtcattaa
      181 gtggataagg ttttagattc agccctcctt tcttcggcaa ataaaagaaa gggtatcctt
      241 gctattggcg cacatcctga cgatatagaa ttaggctgtg gcgcatcgct tgctcgtctt
      301 gcgcaaaaag gaatttatat cgcagcagtg gtgatgacta ccggcaactc tggtacagat
      361 ggaataatcg atcgccatga agaatcgcgc aacgccctaa agatattagg gtgccaccaa
      421 actattcatc ttaattttgc tgacacccgc gctcatttac agctcaatga tatgatttcc
      481 gccctggaag acatcattaa aaatcaaatt ccttctgatg ttgaaatcat gcgggtatat
      541 accatgcatg atgccgaccg ccatcaggat catctcgctg tttatcaagc ttcaatggtt
      601 gcctgccgca ctattccaca aattctcggc tacgaaaccc caagtacctg gctttcattt
      661 atgcctcagg tttttgaatc cgttaaagaa gaatatttca cagtcaaact tgctgcatta
      721 aaaaaacata aaagccagga acgacgtgat tatatgcgtc atgatcgcct gcgtgcagtt
      781 gcacaatttc gcgggcaaca ggtcaatagc gatctgggtg aaggctttgt aattcataaa
      841 atgattcttt gagcaaattg catgaaaacc tggatattta tctgtatgtc catagcaatg
      901 ttgctatggt ttttaagtac gctaagacgt aaacccagtc aaaagaaagg ctgtattgac
      961 gccattatac ctgcgtataa cgaaggcccg tgtctggcgc agtcactgga taatctactg
     1021 cgtaaccctt atttttgccg ggtaatttgc gttaacgacg gctccacgga caataccgaa
     1081 gcggtcatgg cggaagtcaa acgcaaatgg ggcgaccgct ttgttgccgt cacgcaaaaa
     1141 aataccggta aaggtggtgc gctgatgaat ggcctcaatt atgccacctg cgaccaggtt
     1201 tttttaagtg atgccgacac ctatgttccg cccgatcaag acggaatggg ctatatgctg
     1261 gcagaaatta agcgcggtgc tgatgccgta ggcggcattc cctctactgc gttgaaaggc
     1321 gcggggctgt taccgcacat ccgcgcgacc gtaaagttgc cgatgattgt tatgaagcgc
     1381 acgctacagc agctcctggg cggcgcaccg tttattatca gcggtgcctg cggaatgttc
     1441 cgtactgatg tattgcgtaa gttcggtttc tcggatcgta ctaaagtcga agaccttgat
     1501 ctcacctgga cattagtggc aaacggctac cgtattcggc aggcgaatcg ctgcatcgta
     1561 tacccacagg aatgcaacag tccgcgtgag gagtggcgtc gctggcggcg ttggattgtg
     1621 ggctacgcgg tctgtatgcg cctgcataaa aggcttttat ttagccgctt cggtatcttc
     1681 agtatatttc ctatgctgtt ggttgtgatt tatggcgttg ggatttatct cactacctgg
     1741 tttaatgaat tcatcaccac cgggccgcat ggtgtggtgt tggcaatgtt tccgcttatc
     1801 tggatcggcg tagtttgtgt tattggtgct tttagcgcct ggtttcatcg ttgctggttg
     1861 ttggtgcctt tagcgccgct ttccgttgtg tatgtattat tagcttatgc catctggatt
     1921 atctatggac ttattgcctt ttttactgga cgcgaacctc agcgcgacaa acccacccgc
     1981 tattccgcac tggtggaagc gtcaaccgct tattcccaac cttctgtcac aggaactgaa
     2041 aaactatctg aagcttaatg ttaaagagaa aaattattca gattgcagac caggtaatta
     2101 ttgatgaaac tgcaggtgaa gttgttatcg gcgcgaatac ccgtatttgt catggtgccg
     2161 ttattcaggg tccggtagtg attggcgcaa actgcctgat aggtaattat gcgtttattc
     2221 gtcctggcac aataatcagc aatggtgtaa aaattggttt tgccaccgaa attaaaaatg
     2281 cggttattga agcggaagca acgattggtc cgcaatgtta tattgccgac tcggtagttg
     2341 caaaccaggc atatttgggc gcacaagtac gtaccagtaa tcatcgtctg gatgaacagc
     2401 ctgtgtctgt tcgaactcca gagggaatta tcgctaccgg atgcgataaa ttaggttgtt
     2461 atatcgggca gcgttcacgc cttggcgtac aagttattat tttgcctggg cgaattattt
     2521 ccccgaacac gcaacttggc ccgcgcgtga ttgtagaacg taatttacct accggaactt
     2581 attcactccg acaagaactt atccgtacag gagattaaaa tgataaaacg gacgttatta
     2641 gcggcggcca tttttagcgc attgcccgct tatgccgggt taacttccat taccgcgggc
     2701 tacgatttta ccgattattc tggcgatcat ggcaaccgta atttagcgta tgctgaactg
     2761 gtggcgaaag ttgaaaacgc aacgctgctt tttaatcttt cccaggggcg tcgtgattat
     2821 gaaactgaac atttcaatgc cactcgcggt cagggtgcgg tctggtataa atggaataac
     2881 tggctgacaa cccgaacggg tattgccttt gcggataata cgccggtctt tgcccgccag
     2941 gattttcgtc aggatattaa cctggccctg ttgccaaaaa cgcttttcac gaccggttat
     3001 cgctacacta aatattacga tgatgtcgaa gtcgatgcct ggcaaggcgg cgtatcactc
     3061 tatactggcc cggtgatcac cagctaccgc tatacccatt atgactccag cgatgcaggt
     3121 ggtagttata gcaatatgat ttccgtgcgt ctgaatgacc cgcgcggcac tggttatacg
     3181 caactatggc taagccgcgg aacaggcgct tacacctatg actggacgcc agaaacacgc
     3241 tacggcagca tgaagagcgt cagtttgcaa cgtattcaac cgctgactga gcaacttaat
     3301 ctcgggctga cggcaggtaa agtgtggtac gacaccccaa ccgatgatta taacggtctg
     3361 caacttgcag cccatctgac ctggaaattc tgattgcttc tgccgcccgc tatccggggc
     3421 ggccttccct gccgattagc cccactccct ttcctcgttg ttttccgacc acattcaccg
     3481 gataaatttt attctccagc gttatatact atagggaggt atgcattgac atatagcata
     3541 cctccctata gtatattgcg tgcagatgat gaggtgcgaa atgcccagta ctccggaaga
     3601 gaagaaaaag gtccttactc gagttcgtcg tattcggggg cagattgatg ctctggaacg
     3661 gtcgctggag ggtgatgccg aatgccgtgc catactccaa cagatcgctg ccgttcgggg
     3721 cgcggctaac gggctgatgg cagaagtgct tgaaagccat atccgggaaa cgtttgaccg
     3781 aaatgactgc tacagccgcg aagtcagcca atccgttgac gacactattg aactggttcg
     3841 agcctatctt aaatagctga atctattacc atattgagga agagcgagag atgaaatcac
     3901 gtgctgccgt tgcatttgct cccggtaaac cgctggaaat cgttgaaatt gacgttgcac
     3961 caccgaaaaa aggtgaagtg ctgattaaag tcacccatac cggcgtttgc cataccgacg
     4021 catttaccct ctccggggat gacccggaag gtgtattccc ggtggttctc ggtcacgaag
     4081 gggccggcgt tgtggttgaa gtcggtgaag gcgtaaccag cgtcaaacct ggcgaccatg
     4141 tgatcccgct ttacaccgcg gagtgcggcg agtgtgagtt ctgtcgttct ggcaaaacta
     4201 acctctgtgt tgcggttcgc gaaacccagg gtaaaggctt gatgccagac ggcaccaccc
     4261 gtttttctta caacgggcag ccgctttatc actacatggg gtgctctaca ttcagtgaat
     4321 acaccgtggt cgcggaagtg tctctggcca aaattaatcc agaagcaaac catgaacacg
     4381 tctgcctgct gggctgtggc gtgaccaccg gtattggcgc ggtgcacaac acagctaaag
     4441 tccagccagg tgattctgtt gccgtgtttg gtcttggcgc gattggtctg gcagtggttc
     4501 agggcgcgcg tcaggcgaaa gcgggacgga ttatcgctat cgataccaac ccgaagaaat
     4561 tcgatctggc gcgtcgcttc ggtgctaccg actgcattaa cccgaatgac tacgacaaac
     4621 cgattaaaga tgtcctgctg gatatcaaca aatggggtat cgaccatacc tttgaatgca
     4681 tcggtaacgt caacgtgatg cgtgcggcgc tggaaagtgc gcaccgcggc tggggtcagt
     4741 cggtgatcat cggggtggca ggttctggtc aggaaatctc cacccgtcca ttccagttgg
     4801 tcactggtcg cgtatggaaa ggttccgcgt ttggcggcgt gaaaggtcgt tcccagttac
     4861 cgggtatggt tgaagatgcg atgaaaggtg atatcgatct ggaaccgttt gtcacgcata
     4921 ccatgagcct tgatgaaatt aatgacgcct tcgacctgat gcatgaaggc aaatccattc
     4981 gaaccgtaat tcgttactga tttcccgcag gtataccccg tccacttcag acggggttct
     5041 taatactctc cccgggcagc cgtccggggg attaaccgtg agataatgac tgatggaact
     5101 cattgaaaaa catgccagct ttggcggctg gcaaaatgtg tatcggcatt attcccaatc
     5161 actgaaatgt gaaatgaatg tcggcgtcta tctcccacca aaagccgcga atgaaaaatt
     5221 gccggtgctg tactggcttt caggcctgac ctgcaacgag cagaatttca ttactaaatc
     5281 ggggatgcag cgttacgcgg ctgagcacaa cattattgtt gttgcgccgg acaccagtcc
     5341 gcgaggcagt catgtcgcag atgccgaccg ttacgatctc gggcaaggtg ccgggtttta
     5401 cctgaacgca acgcaagcgc cgtggaatga acattacaaa atgtatgact atatccgcaa
     5461 cgagctgccg gatttagtga tgcaacattt tccggcaacg accagaaagt ctatctctgg
     5521 tcattctatg ggcgggctgg gcgcgctggt gctggcgtta cgtaacccag atgaatatgt
     5581 cagcgtctcg gcgttttcgc ccattgtctc cccatcgcaa gtaccgtggg gacagcaagc
     5641 ctttgccgca tatcttggtg aaaataaaga tgcctggttg gattacgatc cggtgagtct
     5701 tatttcacaa ggtcaacgcg ttgcggaaat catggttgat caggggttga gtgatgattt
     5761 ttacgcggaa cagctgcgga ctccaaatct tgaaaagatc tgccaggaga tgaatatcaa
     5821 gacgttaatc cgttatcacg agggttacga ccacagctat tattttgtct ccagttttat
     5881 tggcgagcat attgcctacc acgccaataa actgaatatg cgttgataat agtgcacgac
     5941 tgccggatgc ggcgtgaacg ccttatccgg cctacacttc gcccgtaaac cgtaggcctg
     6001 ataagacgcg caaagcgtcg catcaggcat ggcgcacgac tgccggatgc ggcgtgaacg
     6061 ccttatccgg tctacacttc gcccataaac cgtaggcctg ataagacgcg catagcgtcg
     6121 catcaggcat ctgcgcacga cttatctatg tttaccacat caaatcatca ggcactttga
     6181 agtcggcata cggatcgtct tcatcctgct cttcggcact gagcgcgctg tgcaacacaa
     6241 tactgctggc atcacgctga gcaattttat cggcaacact ggcgggaata atggtgtatt
     6301 caccttcgct attgttatcg accaacaagc gggcaatcgc cagacgacca ttaatcaact
     6361 gcgcctgtgt gagcttatcg acaaaaatct tcttaatcag attgccgtca gtgaagttaa
     6421 aaccaatatc gccattggca atggtgattc ggttcatttc aatgagctgc ttcacctggg
     6481 ctttatattc tttcgccaac gccgcttgtt tttgctgttc gctaagctgt ttatcacgct
     6541 caagctgtgc ctttttattt tcttctaccg cagcccgagc ttcacgcgcc tgaacgcgcg
     6601 attttttcgc cgttctctcg accttcgccg cttttttgct ggtgactaat cctgctttga
     6661 gcaactgctc ttgtaaggta agttttgcca tttttgtttc caaacccgct gaataatttt
     6721 tagcgagtat acctgtaaac gccgtgggtg tattgggggc aagtcccttt cgcccctgct
     6781 taataaggcg cgtcaggcat cggcacacgg ctgcattctt gatctccgac tcatcaaaat
     6841 aaacaccgcc aacccagcaa caagaatacc cggtgcagaa gcggccatta cgccgaccgt
     6901 gccagtgcct aatgccagca ttttcccggc cagtaacgga ccgctcatag cccccagacg
     6961 ccctaccgcc acggctgttc ccacacctgt tgcgcggatc tgcgaactgt aaaacaacgg
     7021 tgccagggca tacaaaacgc tttgcccacc tgtcgcaaac aaccccgcga caaatcccgc
     7081 cagcaacata ccgttaaatg acgacaccgt tccaagcgcc agcagcgaag ctaacatgcc
     7141 gctataaatc agtagcgaca tggttactgg acgcagctta tccatcaatg cgcccaacat
     7201 taacgtcccg cttgccgccc ccatttgcag ggcaaacatc acccctgccg cctgcgatgg
     7261 ctggaatcct tgctccacca aaagtagcgg tagccagttg atcaacatat aaaccaccag
     7321 cagagtgaag aaataacaca accacagcag cagcgtcgcg gttgccgttt ctggcgcaaa
     7381 taaggcacgc agtggtggcg cagcctgttt ttcgccagcg aaaaccgctg actccggcag
     7441 ccagcgcatt aatagcggca ccagaatcaa cggcaccaca ccacctaccc aaaacaccgt
     7501 ttgccatgct aagtttgccc ccgcgaaacc cagtgtcgcc gccagcgccg cgccaatggg
     7561 aacaccgcaa tacatcaggc tcactgccgt cccacgaaaa cgtggacccg cggcttcaga
     7621 tgtcagggcg ataagattcg gcaacgccgc ccccagcccg acaccggtca tcagccgcgc
     7681 aaagaccagt gaggggaaat cccaggcaat cgccgttgcc agtgagaaca aaccaaacag
     7741 cgcaactgag ccaatcaaaa tacgcttacg accataacgg tctgccagca tcccgccaac
     7801 caacgcgccg ggtagcaaac cgagtattcc ggcgctaaat atccagccca tttgcatttt
     7861 atcgagtgcg aaagcctggg cgatgccacc cgccgcaatg ccagccgcct gaagatccag
     7921 cccttccatc agagcgacca aaaaacaaag cccgatggtc agcatcaggc gggatgaaga
     7981 tgatgaaggg gtacgagtcg acataagatg aacctcattg cgagtgcagg tgaatcgcga
     8041 ctgatacatt gccagccgcg taatcagaac tatttaatga ctgcctacgg gcgaaatgta
     8101 gcgaaatgta ggccggataa ggcgttcacg ccgcatccgg cagtcgtgcg cagatgcctg
     8161 atgcgacgct aacgcgtctt atcagaccta caaattctca cgccatccgt aggccggaca
     8221 aggcgttcac gccgcatccg gctgtcgtgc tgggatgggc atacaaaatg cacgttatcg
     8281 gcagtcatca ttatttgttg ttgcgcagat ccagcgccac gtcgacgatc atatcctcct
     8341 ggccgccaac catccggcgt ttgcccagct caacgagaat atccaccgca cttaagccat
     8401 aacgcgccgc cgccgtttca cagtgacgca ggaagctcga gtaaacacca gcgtatccca
     8461 gcgccagcgt ttcgcgatcg actcgtaccg gacgatcctg caacggacgc accaggtcgt
     8521 cggcggcatc cattaacgca tagagatcgg tcccatgctg ccagcccagt ttatccgcgg
     8581 cggcaataaa cacttccagc ggtgcgttac ctgcgcccgc gcccattccc gcgaggctgg
     8641 cgtcgattcg gtcgcagccc tcttccaccg ccgcgataga gttcgccacg ccaagactca
     8701 ggttatggtg agcgtgcatg ccggtttgcg tttctggttt caacactgct ttcagggcgc
     8761 ggaaacggtc acggatatcg ctcatgttca tcgcaccgcc agaatccacc acataaatac
     8821 aggtcgcacc gtagccttcc atcagctttg cctgcttggc gagattctcc ggcgtggtca
     8881 tatggctcat catcagaaaa ccaacggtgt ccattccgag ctcgcgggca tactgaatat
     8941 gctgggcgga aacatcagct tcggtacagt gcgttgccac acgaaccacc cgcgcgccag
     9001 cctgccaggc atttttcaga tcgtgaatag tgccgattcc tggcagcaac aacgtcgcga
     9061 ttttggcgtg cctcaccaca tccgccgccg cttcaatcca ttcaaggtcg ctatgtgcgc
     9121 cgaaaccata gttaaagctg gaaccttgca aaccgtcgcc gtgggccact tcaatcgaat
     9181 ccacgcgggc atcgtccagt gctttggcaa tctggcgaac gttttccagc gaatactgat
     9241 gacgaatggc gtgcataccg tcacgcaatg tgacgtccga gatataaagt tttttaccgt
     9301 tcatgccgct tctcctgcct tgcgcgccag tgactgggcc attttttccg ctgtcgccag
     9361 cgcactggaa gtcataatat cgaggttgcc cgcataggca ggcagataat gcgctgcgcc
     9421 ttcgacttcc agccagaccg ctgttttcag tccggagaat tgccccacgc ccggtaaatt
     9481 gaccggttta tcctgcggga taacttcaaa ctgcacgcgc tgtttcaggc gataacccgg
     9541 tacgtaagcc tgcaccgcct cagccatttc attgattgag gcttcgatat catcttgtga
     9601 agcttcgtcg ctcaatacat acaccgtgtc acgcatcatc aacggtggct ctgctgggtt
     9661 aagcacaata atcgccttcc cttttgccgc gccgcccacc acttcaatgg ctcgggaagt
     9721 ggtttccgta aattcatcga tattggcacg cgtgccaggt ccggcagatt tactggcgat
     9781 agaagcgata atttcggcgt aatgaacacg cgccacgcgt gaaactgccg ccaccattgg
     9841 aatggtggcc tggccgccgc aggtgaccat gttgacgttc agttgatcga cgttcgcctc
     9901 gaggttaacc accggcacgc agtaagggcc gatggcagca ggcgtcaggt caattaagcg
     9961 aatatccggt ttcgcttcgc gtaaagcggc atcgtttttc acatgcgcgc ccgcgctggt
    10021 cgcatcaaat acaatgtcga tatcagcaaa ttcaggcatg ttcatcagcc cgatcacccc
    10081 ttcatgggtg gtggcgacgc ccatacgtct ggcacgcgcc agaccgtcgg actgaggatc
    10141 aatgccgacc atcaccgcca tctccagatg ctgaccgtga cgcaaaattt taatcatcag
    10201 atcggtgcca atgttgccag aaccgataat ggcgacttta cgcttactca tgacagactt
    10261 ccttttgggg ctgcgcttga aaatgtcgca gcaactgaac ctatgccttc aatatgggct
    10321 tcaaaacgat cgcccgcatt caccgccacc atcgggccta atgccccggt aagaatgata
    10381 tctccggcgc gcagcggttc acccagactg gccattttgc gtgccagcca gacggcggca
    10441 ttaagcggat gtcccaggca ttcgctgccg cgcccgctgg aaacctcttc gttattacgc
    10501 gtcatcttca tggcgcagtt tttcaggtct aaccccgccg gacgttgcgc cggaccgccg
    10561 atgacataca ccccgcagga agcgttatct gccacggtat cgacaaactg aatcgaccag
    10621 tcgcgaatgc ggctccccac cacttccagc gccggaagta cccattcaat ggcgttatac
    10681 agttcgtcga aggtgatatc agttgcgggt aaatcgcggt tcaacaccag tgcgatctcc
    10741 gcttcaatgc ggggttgcag aacacgggaa aaaggaatga tttcgttatc gccataacac
    10801 atgtcggcaa ataacgtccc aaaatccggt tgatcaacgc ccagttgttg ttgcactttc
    10861 ggatgcgtca ggcccacttt acgccctacc acgcgacgcc cctgcacaac gtcatattgc
    10921 acatttatgt gctgaatggc gtaggccgct tcagcgtgat cgataccaat cagatcgcgc
    10981 agcggcgcaa tcgcttcgcc ctgctctgcg gcgcggcgta aatccgccgc cagttgctca
    11041 agagtatgct tcgtcatgac cattccttaa gggcgtgcga ggaaattcag caccaattga
    11101 ttgaaagcgt cggcatgttc ccactgcgcc cagtgaccac agtcgcggaa gatatgcagt
    11161 tcagaaccgg caatgccgga cagcagacgc agacccgcat ccatcggcac aaagcggtcg
    11221 ttgcgccccc agacaatcag ggtttgcgct ttgatttccg ccagacgtgg gccaaaatcc
    11281 gggaactgtt tcggattagc ttccaggctc ttaacgaagt tttccaggtg atcgcggcgc
    11341 gacagcatat tattcaggcg cgcttcaaac agggcgtcgg tcaaatcgct ggtatcgaaa
    11401 acgaagatat ccatcatcag cttcaggttt tcgatagtcg gctgacgata aagctgattc
    11461 agtcgcttaa taccttcggt tggcatcggc gtaaacaaac tcatgccgcc cgtaccgccg
    11521 cccatcagca ccagtttgcc gacgcgctcc ggccagttaa gggtgaacgc cacagaacta
    11581 tggccgccca tcgagttgcc cagcaggtgg attttggcga tatccagttg atccaccacg
    11641 cttttcagga ttcgtgcatt aagatccgat cgtgaaccac tattaacgat cgaatcgctt
    11701 ttgccccaac ccggacaatc cagcaggatc acccgatagc ccgcctctac cagcggatcg
    11761 atattgcggc tgaagttcgc ccagccagta gcacccgggc cggaaccatg cagcaggaca
    11821 acggtttcgt cgccttgtcc gcagtcatta aaatggatgc gcagcgtttt acccgcttct
    11881 tctacattca gaaaacggct ggtggcggct tcggtttgtg gctgataact catcatcttc
    11941 tcctgcatat tcagttctct gttctggcgc ttaacgagcc aaatccggca atccactccg
    12001 ggattgggcg gtaataacgc ccttcgctac gccagttgcc aaatgcagaa atagcggcaa
    12061 aagcggcgac ccaggttttg atttcatgtg tcgactttcc ggcaatggcg gaaagctctt
    12121 cgttactgac ggcatccagt tcctgtatgc gtccctgctc cagcaaagtc atgaactggt
    12181 tatcccaaat cgggttgagc ggatgcagag ttctctgatc ctcaacaaac ttctcagcgg
    12241 cgctaatcac cctttgctga cgcaattcgc gctcactggc gggtaaatct ttcccgctcc
    12301 ccaacagacg gtcgcgcata tgggcatcgg ctttcgccag ttcgggcacc ggcggctgat
    12361 gggaaagccc accggaaccc aggaacagca cgcgtttatt gagagtgctg gtgaaacgtc
    12421 caatggcttc acccaacatg cgggtacgct ggaaaccggg cagcggcgtg gcgacaccgt
    12481 tgatgaacac aggcagaact ggcaccttat ccagcccacc gagcaggaac tccagcggct
    12541 gggcgaaccc gtggtccacc tgcatacagt aagaaacggc aagatcgatc ccgctcttca
    12601 tgacagcatg cgcacaggcc tccgccagct ccacaggcac gggcagctct cctgccgcac
    12661 tgccgaaatc accaattgcc gtcgctccaa cgcctaaaca gaacggtggc atcacgtcat
    12721 agaaaaagcc gttgtagtga tctggcgcaa acagcaccac cagttcaggg gagaaagccg
    12781 caatacgctc gcgggcgctg gcaatcacgc cattgacctc atcgagtacc tcttgcgccg
    12841 ggtcgacata ccccaccaac ggcgagtggg aaagacagtg aagataagcg tgcatatcag
    12901 gctacctttt cgacagaaac gtcggcatca gggcgggtca gcgtcatcac cgacgccagt
    12961 ttattcagcg tattgcccag ggtttgcgga atggcggtgg cggcaacaaa gcgatccggg
    13021 cgcatcacca ccagcgaagc attgtgttgc gcgaaccagc tacgcagacg accttgcgta
    13081 tcgcccacgt gtagcacgcc gtcgtggtta tcctgtgcgg tatgaatttg cacttccggc
    13141 accacctgaa tgaagcgtgt gcccaacgcg cgccactgct ggatttgctc gtcgctcatc
    13201 ccccacagtg gattgcatcc ccagccaatt accgcgaagt tcgcgccgat cgcgttatcg
    13261 agcagcgtca cgtcgccgtt ttccagcgtg actttcggct gaataaacat cttgccgacc
    13321 ggagagtgct tcgcttcgcc ctcacgcacc agcgcaccgc cgtaatattg cggcatcggc
    13381 ttgaagcgca tttcgaggaa gtagcgtttt actggcggca gataattcaa cagccaggaa
    13441 acgccgtcac gtaacgtacc ctgccagcgt ttcggcggag ccagcacgtt gcccgccgtc
    13501 acggacaggt caatcatcgc tttggcgtga tcgcgacgtt cttgttgata ggtatcgagc
    13561 agcgcatcgc gggctttccc ctggataacc aacgccagtt tccaggcgag gttaaaggcg
    13621 tcgcgcatac cactgttata gccctgcccc tgccacaccg gcatgatgtg cgcggcatcg
    13681 cccgccagca gtacgcgatc aatacggaaa cgttgcgcca gtcgcgcgtt atgggtgtag
    13741 acacgctggc gaatcaattc aacattgtcc ggattaggca gcactttgct taacagcttg
    13801 cgcatatttt gcggctcacg cagctgctct tcggtttctc ccggcatcac cataaattca
    13861 aagcgacgta ccgcatgagg cagcgcggca gaaacatacg ggcgcaccgg atcgcaacac
    13921 aaatagatat gcggcgtact taacggatcg ttggcgatat ctaccacaat ccactgattt
    13981 ggcgcagttt taccttcaaa cggcacattc agggtgcgac gaacaaaact tgccccacca
    14041 tcacaggcca ccagccactg ggctttgatc gtttcccgct gcccttctgc cgttttcagg
    14101 tgcaaggtca cttcgtcatc ttgctggctg aaggcctcca gctcgcggga aaacaagcag
    14161 cgcacattcg gaaaacgcga caacccttcc agcatcaccg catcgacctg tggctgaata
    14221 aaggcgttac ggcgcggcca gccaaattca tcggtcattg gctgaatatc agcaaaacag
    14281 cggccttttg gggtgagaaa acgcatcgcg tgccacggtg tagtgtgcgg cagaacattc
    14341 tcgaccaggc cgaccgactg catggtgcgc agcgcctcgt catcaatacc aatcgcacgc
    14401 gggtagtcga tcaacttatc gagtttctcc accaccagca cgtcaatgcc catctgaccg
    14461 agatagttcg ccatcatcag cccaaccgga ccggcaccag cgatcgccac ctgaacgcta
    14521 tggttaacag caggctggat gtcagggtgt tgtattgcca tttctgcaca acgttgtttt
    14581 aactatagaa atgtcaatta atatgcagaa caatgagcag acggaataca aaaccgtgcg
    14641 cggcttaacc cgcggtctaa tgttattaaa tatgttaaat aaacttgatg gcggtgccag
    14701 cgtcgggctg ctggcggaac tcagcggcct gcatcgcacc actgtgcggc gactgctgga
    14761 gacgctgcag gaagagggat atgtccgccg tagcccctcc gatgacagtt ttcgtctgac
    14821 catcaaagtg cggcaattaa gcgaaggatt tcgtgacgaa cagtggattt ctgcactggc
    14881 ggccccgctg ctgggcgatc tgttgcgcga agtggtatga ccaacagatg tgtccaccct
    14941 ggatgttgat gcaatggtgg tacgcgaaac cacccaccgt ttcagccgct tatcctttca
    15001 ccgggcaatg gtcgggcgac gtttaccgct cctgaaaacc gcctcgggcc tgacctggct
    15061 ggccttttgc ccggaacaag aacgcaagga attaatcgaa atgttagccg cccgccccgg
    15121 tgatgactat caactggcac gagaaccgtt aaagctgcaa gccattctgg cgcgcgcgcg
    15181 caaagagggt tacggacaga actaccgcgg ctgggatcag gaggagaaga tcgcctctat
    15241 cgccgtaccg ctgcgcagtg aacaacgggt gattggctgt ctgaatctgg tgtatatggc
    15301 gagcgcaatg accattgaac aggcagcgga aaagcatctt ccggcgctac aacgggtagc
    15361 aaaacagatc gaagaagggg ttgaatcgca ggctattctg gtggccggac ggcgaagcgg
    15421 cgtgcattta cgttgacacc atcgaatggc gcaaaacctt tcgtggtatg gcatgatagc
    15481 gcccggaaga gagtcaattc agggtggtga atgtgaaacc agtaacgcta tacgatgtcg
    15541 cagagtatgc cggtgtctct tatcagaccg tttctcgcgt ggtgaaccag gccagccacg
    15601 tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca
    15661 accgcgtggc acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca
    15721 gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac
    15781 tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg
    15841 cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg
    15901 accaggatgc cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg
    15961 tctctgacca gacacccatc aacagtatta ttttctccca tgaagacggt acgcgactgg
    16021 gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa
    16081 gttctgtctc ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa
    16141 ttcagccgat agcggaacgg gaaggcgact ggagtgccat gtccggtttt caacaaacca
    16201 tgcaaatgct gaatgagggc atcgttccca ctgcgatgct ggttgccaac gatcagatgg
    16261 cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gatatctcgg
    16321 tagtgggata cgacgatacc gaagacagct catgttatat cccgccgtta accaccatca
    16381 aacaggattt tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg
    16441 gccaggcggt gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc
    16501 tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
    16561 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
    16621 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgaa
    16681 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cggattcact
    16741 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
    16801 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
    16861 ttcccaacag ttgcgcagcc tgaatggcga atggcgcttt gcctggtttc cggcaccaga
    16921 agcggtgccg gaaagctggc tggagtgcga tcttcctgag gccgatactg tcgtcgtccc
    16981 ctcaaactgg cagatgcacg gttacgatgc gcccatctac accaacgtga cctatcccat
    17041 tacggtcaat ccgccttttg ttcccgcgga gaatccgacg ggttgttact cgctcacatt
    17101 taatattgat gaaagctggc tacaggaagg ccagacgcga attatttttg atggtgttaa
    17161 ctcggcgttt catctgtggt gcaacgggcg ctgggtcggt tacggccagg acagccgttt
    17221 gccgtctgaa tttgacctga gcgcattttt acgcgccgga aaaaaccgcc tcgcggtgat
    17281 ggtgctgcgc tggagtgacg gtagttatct ggaagatcag gatatgtggc gaatgagcgg
    17341 cattttccgt gacgtctcgt tgctgcacaa accgagcaca caaatcagcg atttccatgt
    17401 tgccactcac tttaatgatg atttcagccg cgcggtactg gaggcagaag ttcagatgta
    17461 cggcgagctg cgcgatgagc tgcgggtgac ggtttctttg tggcagggtg aaacgcaggt
    17521 cgccagcggc accgcgcctt tcggcggtga aattatcgat gagcgtggtg gttatgccga
    17581 tcgcgtcacg ctacgtctga acgtcgaaaa cccggcgctg tggagcgccg aaatcccgaa
    17641 tctctatcgt gcggtggttg aactgcacac cgacgacggc acgctgattg aagcagaagc
    17701 ctgcgatgtc ggtttccgcg aggtgcggat tgaaaatggt ctgctgctgc tgaacggcaa
    17761 gccgttgctg attcgcggcg ttaaccgtca cgagcatcat cctctgcatg gtcaggtcat
    17821 ggatgagcag acgatggtgc aggatatcct gctgatgaag cagaacaact ttaacgccgt
    17881 gcgttgttcg cattatccga accatccgct gtggtacacg ctgtgcgacc gcttcggctt
    17941 gtacgtggtg gatgaagcca atattgaaac ccacggcatg gtgccaatga atcgtctgac
    18001 cgatgatccg cgctggttac ccgcgatgag cgaacgcgta acgcgaatgg tgcagcgcga
    18061 tcgtaatcac ccgagtgtga tcatctggtc gctggggaat gagtcaggcc acggcgctaa
    18121 tcacgacgca ctctatcgct ggatcaaatc tgtcgatcca tcccgcccgg tgcagtatga
    18181 aggcggcgga gccgacacca cggccaccga tattatttgc ccgatgtacg cgcgcgtgga
    18241 tgaagaccag cccttcccgg ctgtgccgaa atggtccatc aaaaaatggc tttcgctacc
    18301 tggagagacg cgcccgctga tcctttgcga atacgcccac gcgatgggta acagtcttgg
    18361 cggcttcgct aaatactggc aggcgtttcg tcagtacccc cgtttacagg gcggcttcgt
    18421 ctgggactgg gtggatcagt cgctgattaa atatgatgaa aatggcaatc cgtggtcggc
    18481 ttacggcggt gattttggcg atacaccgaa cgatcgccag ttctgcatga acggtctggt
    18541 ctttgccgac cgcacgccgc atccagcgct gacagaagca aaacaccagc agcagttttt
    18601 ccagttccgt ttatccgggc aaaccatcga agtgaccagc gaatacctgt tccgtcatag
    18661 cgataacgag cttctgcact ggtcggtggc gttggatggc aagccgctgg caagcggtga
    18721 aatgcctctg gatgttgctc cacaagataa acagttgatt gaactgcctg aactaccgca
    18781 gccggagagc gccggacaac tctggcttac ggtacacgta gtgcaaccga acgcgaccgc
    18841 atggtcagaa gccggacaca tcagcgcctg gcagcagtgg cgtctggcgg aaaacctcag
    18901 cgtgacactc cccgccgcgc cccacgccat cccgcaactg accaccagcg aaacggattt
    18961 ttgcatcgag ctgggtaata aacgttggca atttaaccgc cagtcaggct ttctttcaca
    19021 gatgtggatt ggcgctgaaa aacaactgct gacgccgctg cgcgatcagt tcacccgtgc
    19081 accgctggat aacgacattg gcgtaagtga agcgacccgc attgacccga acgcctgggt
    19141 cgaacgctgg aaggcggcgg gccattacca ggccgaagca gcgttgttgc agtgcacggc
    19201 agatacactt gccgacgcgg tgctgattac gaccgctcac gcgtggcagc atcagggaaa
    19261 aaccttattt atcagccgga aaacctaccg gattgatggt agtggtcaaa tggcgattac
    19321 cgttgatgtt gaagtagcga gcgatacacc gcatccggct cggattggcc tgacctgcca
    19381 gctggcgcag gtagcagaac gggtaaactg gctcggatta gggccgcaag aaaactatcc
    19441 cgaccgcctt actgccgcct gttttgaccg ctgggatctg ccattgtcag acatgtatac
    19501 cccgtacgtc ttcccgagcg aaaacggtct gcgctgcggg acgcgcgaat tgaattatgg
    19561 cccacaccag tggcgcggcg acttccagtt caatatcagt cgctacagcc aacaacaact
    19621 gatggaaacc agccatcgcc atctgctgca cgcggaagaa ggcacatggc tgaatatcga
    19681 cggtttccat atggggattg gtggcgacga ctcctggagc ccgtcagttt cagcggaatt
    19741 ccagcttagc gccggtagct accattacca gttggtctgg tgtcaaaaat aataataacc
    19801 gggcaggcca tgtctgcccg tatttcgcgt aaggaaatcc attatgtact atttaaaaaa
    19861 cacaaacttt tggatgttcg gtttattctt tttcttttac ttttttatca tgggggccta
    19921 cttcccgttt ttcccgattt ggctacatga catcaaccat atcagcaaaa gtgatacggg
    19981 tattattttt gccgctattt ctctgttctc gctattattc caaccgctgt ttggtctgct
    20041 ttctgacaaa ctcgggctgc gcaaatacct gctgtggatt attaccggca tgttagtgat
    20101 gtttgcgc
//
LOCUS       DAZPDU010000071        19796 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZPDU010000071 DAZPDU010000000
VERSION     DAZPDU010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19796
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.072"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            236..1138
                     /locus_tag="ACHURS_002285"
     CDS             236..1138
                     /locus_tag="ACHURS_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1238001.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPVFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            1285..2505
                     /locus_tag="ACHURS_002286"
     CDS             1285..2505
                     /locus_tag="ACHURS_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFR1238002.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKNSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPV
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQNLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            2478..3107
                     /locus_tag="ACHURS_002287"
     CDS             2478..3107
                     /locus_tag="ACHURS_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFR1238003.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGFTQPIVVTHTDKNAMEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVICLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(3108..4268)
                     /gene="ybdL"
                     /locus_tag="ACHURS_002288"
     CDS             complement(3108..4268)
                     /gene="ybdL"
                     /locus_tag="ACHURS_002288"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFR1238004.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNSSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHSQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            4377..5465
                     /gene="hcxA"
                     /locus_tag="ACHURS_002289"
     CDS             4377..5465
                     /gene="hcxA"
                     /locus_tag="ACHURS_002289"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFR1238005.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLNDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEMDINNHAEIDKVITHTLRP
                     VEYIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(5475..5672)
                     /locus_tag="ACHURS_002290"
     CDS             complement(5475..5672)
                     /locus_tag="ACHURS_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFR1238006.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(5854..7959)
                     /gene="cstA"
                     /locus_tag="ACHURS_002291"
     CDS             complement(5854..7959)
                     /gene="cstA"
                     /locus_tag="ACHURS_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFR1238007.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKSALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(8140..8553)
                     /gene="entH"
                     /locus_tag="ACHURS_002292"
     CDS             complement(8140..8553)
                     /gene="entH"
                     /locus_tag="ACHURS_002292"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFR1238008.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIIVFDEQGRRCCTCRLGTAVLG"
     gene            complement(8556..9302)
                     /gene="entA"
                     /locus_tag="ACHURS_002293"
     CDS             complement(8556..9302)
                     /gene="entA"
                     /locus_tag="ACHURS_002293"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFR1238009.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAVSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(9302..10159)
                     /gene="entB"
                     /locus_tag="ACHURS_002294"
     CDS             complement(9302..10159)
                     /gene="entB"
                     /locus_tag="ACHURS_002294"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFR1238010.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEGLLPAPVPARKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(10173..11783)
                     /gene="entE"
                     /locus_tag="ACHURS_002295"
     CDS             complement(10173..11783)
                     /gene="entE"
                     /locus_tag="ACHURS_002295"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFR1238011.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLLNDSGE
                     HNLQDAINHPADGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARISAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(11793..12968)
                     /gene="entC"
                     /locus_tag="ACHURS_002296"
     CDS             complement(11793..12968)
                     /gene="entC"
                     /locus_tag="ACHURS_002296"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFR1238012.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSAHCFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            13343..14308
                     /gene="fepB"
                     /locus_tag="ACHURS_002297"
     CDS             13343..14308
                     /gene="fepB"
                     /locus_tag="ACHURS_002297"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFR1238013.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSVLALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYTLGTETFRLDYYSAMQVLDRLNSLLKKD"
     gene            complement(14389..15639)
                     /gene="entS"
                     /locus_tag="ACHURS_002298"
     CDS             complement(14389..15639)
                     /gene="entS"
                     /locus_tag="ACHURS_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFR1238014.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            15750..16754
                     /gene="fepD"
                     /locus_tag="ACHURS_002299"
     CDS             15750..16754
                     /gene="fepD"
                     /locus_tag="ACHURS_002299"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFR1238015.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFIGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            16751..17743
                     /gene="fepG"
                     /locus_tag="ACHURS_002300"
     CDS             16751..17743
                     /gene="fepG"
                     /locus_tag="ACHURS_002300"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFR1238016.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            17740..18555
                     /gene="fepC"
                     /locus_tag="ACHURS_002301"
     CDS             17740..18555
                     /gene="fepC"
                     /locus_tag="ACHURS_002301"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFR1238017.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATRITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(18552..19685)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHURS_002302"
     CDS             complement(18552..19685)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHURS_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFR1238018.1"
                     /translation="MSSLNIKQGSDAHFSEYPLASPSNNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSSAVITPAEAIQWQDLEKTFTKLRVLDLDVNIDRGG
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
BASE COUNT         4805 a         5497 c         4995 g         4499 t
ORIGIN      
        1 catcacccat tatgggaaat aaataaaagg taattcatgg atggttgaac tatatccctg
       61 cttttcaacc atcctaaaaa agcatgaatt gctcgctcta ccctaagtgg ctgtgctatt
      121 ttagaaaaac ttaatcctga gttaagcaaa aaaacataat caataaaaat acatacgcaa
      181 aaaacattga ttaagtgaat ataccatgga agaaaaatat aaaccggagt agtgtatggc
      241 caatctctac gacttgaaaa aattcgactt aaatcttctt gtcatatttg agtgtattta
      301 ccagcattta agcattagca aagctgctga atcactatat atcaccccat cagccgtgag
      361 ccagtcgtta caacgcttac gtgcgcaatt taatgaccct gtattcattc gctcaggaaa
      421 aggtatcgcg ccgacgacaa cagggttgaa tctgcatcac catcttgaga aaaacctcaa
      481 aggccttgaa caaacaatta atatagtcaa taagtccgaa ctaaagaaaa actttattat
      541 ctacggccct cagctcattt cttgctctaa taatagtatg ctgatccgct gcctacggca
      601 agatgcctct gtcgagattg agtgccatga tatcctcatg tcagcagaga atgctgaaga
      661 actgttggtt caccgcaaag ccgatctggt gatcacccaa atgccagtga tcagccgttc
      721 cgtcatttgc atgccattgc atacaattcg caatactcta atttgtagta ataagcatcc
      781 gcgaattact gacaatagca cttatgaaca aattatggca gaagagttca cccaattaat
      841 atccaaatca gcaggagttg atgatattca aatggaaata gacgagaagt ttatgaatcg
      901 caaaatttca tttcgtggtt cctctttgct gacaataatt aatagcattg cagttacaga
      961 tttactggga atagtcccct atgaacttta taactcccac cgtgactttc ttaatttaaa
     1021 agagataaaa ctagaacatc cgttaccttc aataaagctt tacatttcct ataataaatc
     1081 ttctttaaat aatttggtgt tctcccgatt tattgaccgt ctaaatgaga gtttttgata
     1141 tataactaca gacagctact ataactttca tctatttatt cacagcgcga atgaaaaata
     1201 aaacactctg ctttacagtg tttactttat aattaacagt cttatctcac tgttcaccct
     1261 gtaacgttca gagagcgtct tcagatgtct atttataaaa ttcctcttcc gctcaatatt
     1321 ctggaagctg cacgagagcg tattacctgg acactcaaca ccctaccccg cgtttgtgtc
     1381 tcattttctg gcggcaaaaa ctctggttta atgctgcatc tgacagccga acttgcccga
     1441 caaatgggca aaaaaatctg cgttttgttt atcgactggg aggcgcaatt ctcttgcacc
     1501 attaactatg ttcagtccct gcgcgagttg tacaccgatg tcatcgaaga gttttactgg
     1561 gttgcgctcc cgcttacgac gcaaaattcc ctttcacaat accaacccga atggcagtgc
     1621 tgggaacctg atgtcgaatg ggtgcgtcag cccccgcaag atgcgataac cgatcctgac
     1681 tttttctgct tttaccagcc aggcatgacc ttcgaacaat ttgtacgtga gtttgccgaa
     1741 tggttttcac aaaaacgtcc ggtggcgatg atgatcggca tccgtgcgga tgagtcctac
     1801 aaccgttttg tcgccatcgc cagtttaaat aaacaacgtt ttgccgacga taaaccatgg
     1861 actacggccg caccaggcgg tcatagctgg tacatttacc ccatttacga ctggaaagtg
     1921 gctgatatct ggacctggta tgcaaatcat caaaatctct gcaacccact gtataaccta
     1981 atgtatcagg caggcgttcc tctgcgtcat atgcgaattt gcgaaccttt tggcccggag
     2041 caacgacaag gattatggct ctatcacgtt atcgaaccgg atcgctgggc tgctatgtgc
     2101 gcacgagtca gcggggtaaa aagtggcggc atttacgccg gacatgataa tcatttctat
     2161 gggcatcgga aaatcctcaa gccagaacat ttagactggc aagaatatgc attattgctg
     2221 ctcaatagca tgccggaaaa aacagctgag cattaccgca ataaaatcgc catttatttg
     2281 cactggtatc agaaaaaagg catcgaggtt ccacaaaccc agcaagggga cattggcgcg
     2341 aaagatatcc cctcctggcg gcggatatgc aaagttttac tcaataacga ttactggtgt
     2401 cgggcattat catttagtcc tacgaaagcg aagaactatc agcgttataa cgaacggata
     2461 aaaggaaaac gtcaggaatg ggggatacta tgcaacaacg attaacgcag gatttaacgc
     2521 aatttctcgc cagcttgccg gaagatgatc gcatcaaagc gattaatgaa atccgcatgg
     2581 cgatccatca ggttagcccc tttcgtgaag agccagtgga ttgcgttctg tgggttaaaa
     2641 acagccaact tatgcctaat gattacaacc caaacaacgt ggcaccaccc gaaaaaaaac
     2701 tgctgcaaaa atccattgaa atagatggtt ttacccaacc cattgtggtc acacatacgg
     2761 ataaaaatgc catggaaatc gtggatggtt ttcaccgcca tgaaattggt aaaggctcat
     2821 catcattaaa attacgctta aaaggttatt tgccggtgat ctgtctggaa ggtacgcgta
     2881 atcaacgcat cgccgcgact attcgtcata atcgcgctcg tggacgccat caaatcaccg
     2941 ccatgtcgga aatcgtccgt gaacttagcc agttgggatg ggacgataat aaaattggca
     3001 aagagctggg aatggacagc gacgaagtgc tgcgcctgaa gcaaattaac ggcctgcaag
     3061 agctgtttgc cgaccgtcaa tattcccgcg cctggacggt taaataacta aagctggcgc
     3121 aggcgttcag ctgctgccag caacgtcgat tccttcttgg caaaacagag acgaatcagt
     3181 ttatgtggga agggatcggc gcaaaacacc gacagcggaa tcgccgctac gccgtgctcc
     3241 tgcgtcagcc actggcaaaa ctcaacatca tccagggtag aaaccgcgct gtaatccacc
     3301 agcaaaaagt atgtaccttc acacggtaaa atctccagcc ggctttcatt taaggcattt
     3361 accagaatat cgcgcttctg gcgataaaag tccggtaacg caagataatg ctcaggttct
     3421 gcacgtagca tatcagcaag cgccagctgt gccggggtat tcaccgaaaa ggtcagatac
     3481 tgatgtacct tgcgaatttc ggcgctgatg ggcgctggcg caacacaata acccactttc
     3541 cagccggtca tatgataggt cttgccaaat gaagaaaccg ccactgcccg ctcacgcagc
     3601 tgcgaatgcg ccagcacact ggcatggccc tgttgtgaaa agttgatgtg ctcgtagact
     3661 tcatcgctaa tgacaaaaat ctcgtgcccg gcgatcgcct gccacaaagc ggcgaaatca
     3721 gcctgctgcc agacagttgc actggagtta tgcggagtgt tgaggatcac cagtctggtg
     3781 cgctcgctta ataatgcggc aaattcctgc cagtcaacgc gaaaatgcgg tggttgcagt
     3841 gccatacgct tcactattcc cccagaaagc gcgatggcgg gggcgtaact gtcatagctg
     3901 ggatcaaaac aaatcacttc atcgccattg cgcaccagtg cggtaatcgc cgcgtataac
     3961 gcttccgtcg cccctgccgt tacggtgata tcgctatcgg catctggttg atagccatac
     4021 aaacgttccg ttttctgagc aatcgcctcg cgcaaggcct gcacgccggt catgggcgcg
     4081 tattggtttg ccccctgggc aacgtggtac gccagccgct cctgtaaata gcgcggacca
     4141 tcaaaatcag gaaagccttg cgacaggtta atcgcctggt gttgctgcgc cagcgcgctc
     4201 atctgggtga aaatagtggt gccaagttgt ggaagtttgc tttgtggaat cagagggtta
     4261 tttgtcattt ttattgtacc tgacgaagtt gcgtgttgaa ggcactataa cacgatgcta
     4321 gtatttggca atcaagacgt ttagatgtct aaataaaaca ataaggacaa cacaacatgc
     4381 ctcacaatcc tatccgcgtg gtcgtcggcc cggctaacta cttttcacat ccaggaagtt
     4441 tcaatcacct gaacgatttt ttcactgatg aacaactttc tcgcgcggtg tggatctacg
     4501 gcaaacgcgc cattgctgcg gcgcaaacca aacttccgcc agcgtttgga cttccagggg
     4561 caaagcatat tttgtttcgt ggtcattgca gcgaaagcga tgtacagcaa ctggcggctg
     4621 agtccggtga cgatcgcagc gtggtgattg gcgtcggcgg cggcgcactg ctcgacaccg
     4681 cgaaagccct cgcccgccgt ctcggtctgc catttgttgc cgttccgacg atcgccgcta
     4741 cctgcgccgc ctggacaccg ctctccgtct ggtataacga tgccggacag gcgctgcatt
     4801 atgagatttt cgacgacgcc aattttatgg tgctggtgga accagagatt atcctcaacg
     4861 cgccgcaaca atatctgctg gcggggatcg gtgacacgct ggcgaaatgg tatgaagcgg
     4921 tggtgctggc tccgcaacca gaaacgttgc cgctaaccgt gcgactgggg atcaataatg
     4981 cgcaggccat tcgcgacgta ttgttaaaca gtagcgaaca ggcgctgagc gatcagcaaa
     5041 atcaacagtt aacgcaatca ttttgcgatg tggtggatgc tattattgct ggtggtggga
     5101 tggtcggtgg tctgggcgat cgttttacgc gtgtggcggc ggctcatgcc gtgcataacg
     5161 gtctgactgt cctgccgcaa accgagaaat ttctccacgg caccaaagtc gcttatggca
     5221 ttctggtaca aagcgccttg ctaggtcagg atgatgtgct ggcgcaatta accggggcgt
     5281 atcagcgttt tcatctgccg accacgctgg cggagctgga aatggatatc aataatcatg
     5341 cggagatcga caaagtgatt acccacaccc tgcgtccggt ggaatacatt cattacctgc
     5401 cagtcacgct gacaccagat acgttgcgtg cagcgttcga aaaagtggaa tcgtttaaag
     5461 cctgacgtgc aaatttagca gcagcgcgcg ccgccttttc caccgtagcg cgcgtcctgc
     5521 cgctcacgga aaaactcttc gtaggtcatc ggcgtttgat cgggatggtt aacccgcata
     5581 tgttcgacat agttgtcgta atcaggcata ccaatcatca gcttcgccgc ctgacctaaa
     5641 tattttccgg cttttgccag tgaatcaaac atcttggcct ccgctaacca tgttgctgaa
     5701 agagtgccgg atgcggcgtg aacgccttat ccggcctaca aatgctatca acgcgctcgg
     5761 tcagtcccct cgccccatcg gggagagggt tagggtgagg aggaaccctc ggcactatgc
     5821 caacattcgc caacatcccc cctcactctg actttagtgt gcgccttttg cctgcgccac
     5881 gatctcctcg acattttccg gcattggctc atacggcgtt tctttcgccg ttggcttcgg
     5941 atctttcaat gccgccagtg ccgacttaat cgagaacagc gccagaacca ccacgaccac
     6001 cataaagaag atggttaacc cggcatccag acggttgttg aacaccagtt gcgccagctg
     6061 tgactcagta tactgcgacg gaatattgcc gctgtcgatc attgcctgga acttattagc
     6121 aatggccagg aagccgactt tcgcatccgg gctaaacgct ttctgccagc ctgcggtcag
     6181 ggtacaaatc agcagccagg ccgttggtac cagcgccacc caggcgtaac gttgacgttt
     6241 catcttgaac aacaccacgg cacagagcat cagcgccatc cctgccagca tctggttggc
     6301 aataccaaac agcggccaca gagtgttaat gccgcccagc ggatcgacca cgccctgatg
     6361 gaggaagtag ccccacgcca gtacgcacag cgctgttgcc agcaggttag caggcagtga
     6421 atcggtccgc ttcaggccag gagacaccac gcccagcaga tcctgcaaca taaagcgcgc
     6481 agcacgcgta cccgcatcta ccgccgtcag aataaacaga gcttcaaaca gaatggcgaa
     6541 gtgataccag aacgccacat ccatcatacc acccagcgca ccgtgcagga tgtaagccat
     6601 ccccaccgcc agcgtcggcg caccgcccgc acgggaaatg atcgactgtt cacccacttc
     6661 gctggcaatc tggttgagcg tatccggagt aatcgcaaag ccccagctac tcaccacctg
     6721 cgcggcagaa gcgaccacat ccgccgtccc tgccggagcc agcaccgcca tcgggctgtt
     6781 catggcgaag tacacgcccg gatcgatgat acaggcggaa accagtgcca taatcgccac
     6841 gaaggattcc attaacattc caccgtagcc gataaagcac gcctgccctt cgttcgccag
     6901 catcttcggc gtggtgccgg aagagatcag cgcatggaag cctgacaccg cgccacaggc
     6961 gatggtgata aacaggaacg ggaacagatt accggtccat accgggccag tgccatcgac
     7021 aaatttggtc agcgcaggca tggtcagcgt cggacgcata atcaaaatgc ctaccgccag
     7081 accaacgata gtcccgattt tcaggaaggt agagaggtag tcacgcgggg ccagcagtaa
     7141 ccacaccggc agcaccgccg ccacaaaacc gtagcccacc agcatccagg tcagctgcac
     7201 gccggtaaag tcaaagtacg gtgcccaggt cgggctttct gccacccagc cgccagagat
     7261 aatggcaaaa ataaggaaca ccaggccaat cactgacact tcgccaatac gccccggacg
     7321 cagatagcgc aggtagattc ccataaaaat cgccagcgga atggtgaacg caacggtata
     7381 cgttccccac gggctatgag tcagggcttt caccacgatc atcgccagta ctgcaaggat
     7441 aatgaccatg atcataaagc aggctaccag cgcaatcacc ccggcagttg gccccatctc
     7501 ttctttgacc agctcaccca gcgagcgacc gtcacggcgc gtagaaacaa acagcaccat
     7561 gaaatcctgc accgcaccgg cgagaaccac cccggcgagc agccagatca tccccggcag
     7621 gtagcccatt tgcgctgcca gcaccggccc caccagcggc cctgctccgg caatggccgc
     7681 aaaatggtga ccgaacagca ctttcttgtc cgtcggcaca tagtccagcc cgtcgttatg
     7741 gcgcaccgct ggcgtcatac gcgtcgggtc aaccgccagc acatttttgg cgatatacag
     7801 cccataaaaa cggtaagcga tcagatagat acagaccgac gccaccacaa tccacagcgc
     7861 gttgatctgt tccccacgat ttaaagcaat gtatcccaga gcaaatgctc ccattacaga
     7921 gagcactgtc cagacgaggt atttccctga tttgttcata gttgttatcc gtgtgcgtgt
     7981 ccatagagat gttacatttt gtttctataa catctttgta cgatttaaca acacggtaac
     8041 catgtaaatc ggagttgaaa ccaatattta acttaacaat gttaactcga tcactccgtt
     8101 aaccgagttt tcctgcaatc tcatttaatt ctgtcgggct catcccaaaa ctgccgtacc
     8161 cagccgacaa gtgcagcaac gccgcccctg ttcatcgaaa acgatgattt cccagctctg
     8221 attttgccgc ccaagatgca gcggctggca gacgccgcgt accttgcctt cagacaccgg
     8281 gcgatgatgg gtggcgttaa gttccgtacc caccacacac tgcccgtcac gggtcatcat
     8341 aaatccggcc atcgatccca gcgtttcagc cagcgccgcc gacgcgccgc catgcagcag
     8401 gccaaacggc tgatgagtac gggtatcaac cggcatttcg gcttccagca catcatctcc
     8461 cagacgggta tacacaattc ccagatgcgc caccattgtg ttatcgctgg tggcgttcag
     8521 ttcgtcgagc gttaaatggc gtttccagat catgcttatg cccccagcgt tgagccgcca
     8581 tcgaccacaa tatcctgtag ggtgatatgg ctggcgaggt cagaggcgag gaacaaaatc
     8641 gtgttggcga tctcttgtgg acgggcgatt ttccccagcg gaatgccgag tttaaactgc
     8701 tcgccaaagc cgcgaatacg ctgttcttcg gcgtcatcgc tcacccacag cgtgcgttgc
     8761 atatcggtgt cggtggagcc aggcgaaacc acattacagc gcacgccgct aaccgccagt
     8821 tccagcccga cgctcaacgc caggcttttc agcgccgctt tcgatgcgcc ataagcactc
     8881 ataccaatac gcggcgtgtg tgcggcgtcg gacgccacag tgacaatcgc cccgccccgc
     8941 tgacggcgaa actggttcat ggtttgctga aacaggttaa acgcaccgcc gacgttaacc
     9001 gcaaaagtct gctgccagtc ctctttactg agctgatcgg tcgcgcccat gcgcaaaatt
     9061 cccgccgcat tgaccagcac gtccagtcgc tccgtttcag ctaacagtcg ctgacatacc
     9121 tgcgcgacct gcgcagcatc ggcaacatcc atcacttcgg tcgcaaaggg atattgctcc
     9181 tgagcgaacg cttgatcaaa accggtaact ttcgctcccg cttcaacaaa cgccagcgcc
     9241 gtggcgtagc cgataccttt acctgcgccg gttacccaga catttttacc gctgaaatcc
     9301 attatttcac ctcgcgggag agtagcttcc accaggcgtc gatggtcggg tttttcgcca
     9361 gcatgacaaa gtcgatatcg ccatgcactt tgcgccagcg cgccgccagc gccatcatgc
     9421 gcaccgaatc cagaccgtag tcgatcaggt tgtcgtcatc gaacggttca tcggactcgt
     9481 ccagcaacgg caggataacc tcacgcagcg ccgctttcct ggcggggaca ggtgctggca
     9541 gtaatccttc agtcatcacc acccggccag accgcccggc cacatatttc agcgacatca
     9601 aatgctcgtc acggctgaaa tcggccagcg catccgccac cataaacggt ttaatatcgc
     9661 gcataaatgc gtcggttgcg gtggtcatac agccaatgtg ggcatatacc ccggtaataa
     9721 tcagctggtt acgtccactc tctttgagca tttgctccag cggagaacga tgaaacgcgc
     9781 tgtagcgcca cttcaccagc accgtgtcgt cggcatctgg cgtcaggcga tccaccacct
     9841 tttgctgttc cggcgaacgg gtcaggcccg gcccccacat atcattcaac agcgcccgat
     9901 cttcatcgct ctgctctttc ggctgggcgg tgtaataaac cgggatattg tgctgtttgc
     9961 agtagtcgcg cagcgcagca atattcgcga tcacctgctc catcatcggg cagttctcgc
    10021 cccagaagct gacaaaatag tcctgcatat catggattaa caacgcggca cgttgcggtt
    10081 caaaggccca gtcaacttta ttctgcggaa tatcgtgaga ctccggcagt gcgtaagcct
    10141 gtaattttgg aatagccatc gtgttctctc cttcaggctg atgcgcgtga cgccagccac
    10201 tgacgtaatt gttttttatc gactttcccg accgccgtca gcggaagtga atccacacac
    10261 tccacgcgat ccggtaattt aaattcggca ataccctgtt cacgcaggaa acgacgcacc
    10321 tgcaccgcgc gcagcggctc ttttaccacc agataagcac agcttttttc gcccatcagc
    10381 tcatcttcca tgctcaccag tgcggcatag atcaccgccg gatggcgcag cagcaggttt
    10441 tcgatctctt cggcagcgat cttctcgccg ccacggttga tctgatcttt ctcgcgcccc
    10501 tgcacggtga tgtaaccctc tggatcaata gagatcagat cgccggaaca gtaaaaaccg
    10561 ttggcatcaa aggcactggc attgtgctgc ggacttttgt aataaccgcg gaaggtgtac
    10621 ggcccgcgcg tcatcaggcg tccaacttcc ccttgcggca gtggatttcc ttcagcatca
    10681 gcaacccata cttcatcgtc cggacacatt gggtaaccct gggtatggat aattttctcc
    10741 gcgctatcat caagtcgggt gtagttcacc agcccttccg ccatgccaaa cacctgctgc
    10801 aactggcagc caatctcagc ggaaatacgc gccgcaaggg tggcagaaag acgtgcgccg
    10861 ccgacctgta acagtttcag cgaggcaagc tgcgcccggc tttcgccttc agccagcgcc
    10921 tgcaaccaca ggctgactgc cggcggcacc agcgcggtaa cgttaacctg atgttttgca
    10981 atcaacggga agcaaagcgt ggcgctggga tcggccgcca gaacaaccgt tcctccggca
    11041 agaaagacgc ccagcgatcc tggcgaactc atagcgtagt tatgagccgc cgggatcgcg
    11101 cacaggtagc gtgtctgttg tgtgaactga caaatctcga cgctacgacg cacgctgtag
    11161 tagtagtcgt tatgagtgcg cgggatcagc ttcggtgtac cggtggtgcc gccggaaagc
    11221 tggaaatagg ccacttcatc agcaggtgat ggcgtagcag taaaaccgtc tgccggatgg
    11281 ttaatcgcat cctgcaagtt atgctcaccg ctgtcgttga gcagttgcac cacgcgaatg
    11341 gaggaatgtt ctgtgacgaa agtattgagg aaatcatccc cgctaaacag cgcatgttgg
    11401 cgatcggcaa tcagtaacgc cggttcaatc tggctggcat aggcgttcag ttcactacgc
    11461 tgatggctga acaacgccag caccggcgca acgcccagtt tcagcagtgc gaaaaaggta
    11521 atgtaaagtt cagcgacgtt acccagttgt accagcgcgg tttcaccggg tttaatgccc
    11581 tgacggcgta aactacacgc gaggttatcc gccgcctgat tcagctcccg gtaactcaac
    11641 tgtcgctcgc cgtcgataac cgcgatgctg tcactcgcag cgtggcgagt cagaatgtcg
    11701 gtcagcggca aatcctgcca gtagcctttt tcccgatagc gacgggcaaa ctcttccggc
    11761 cagcgggtga atggaatgct catcctcgct ccttaatgca atccaaaaac gttcaacatg
    11821 gtagaaagtt tgacgcctgt ttcgcgccac tcacccaacg gtgacgacgc aggcacaatc
    11881 cccgctccgg caaacagacg cacctgattt tcccgcagct tcgcgcagcg gatggtcacc
    11941 acccattcgc cgttaccttc gctgtcacac caacccacaa tgccgccaaa cagttcgcgg
    12001 tcaaacggct ccagttcagc aataacctgg gtcgcggcct gatgcgggaa accgctcagc
    12061 gcgggggtcg gatgcagcag acaggccaga gtcagtgcgt tttcttgcga attcgcttta
    12121 ccttcaaagg gagttgcgag atgccacagc gtcggcgtgg taatcaattg tggagaggag
    12181 ggaacgtgta actcactact gcgttcgcgc agtacctctt tcatcgcctg agtcaccagt
    12241 tcatgttcat ggcgatcttt ttctgacgcc agcagacgat tacccgcttc gcgatcgagc
    12301 acttcatccg gctgacgacg cgcggaaccg gctaacggaa tggagctaaa acgctcgccg
    12361 tctttacgta gcagcagttc cgggctggcc cccagcagga cgccaccatc agccagcggg
    12421 acatggaagt tgtaactaac cgggttttgc gcaatcaacc gttccagcaa tacgccacta
    12481 tcaatggcgg cgtcagtggt gatatcaatc aatcgtgaca acaccacttt gtcgacctgc
    12541 ggcgtggcgg taagtgcggc ggcgcgggca accatctgtt caaacgtggt ttgttcagga
    12601 attgcctggc gttccaccac gttcagcgac tggctgcggg taaaacagtg ggctgaggtc
    12661 tgtttttcct gacgggagaa cgactgccag gattcgggaa tatacagcga cgaaggctga
    12721 cgtggatcga agggaatcgc cccgaccatc accggatttt tgatgccctg cgctttggca
    12781 tcggcaaaca gcgcggcgag tttttgctgg aagggactgt cgggcgaatc cccgttcaca
    12841 gccggttcat cgaagcgggc gaaacatcct gacgtcgtaa aactgcggta cggcgacata
    12901 aagaaaaagc gattgggcgc aagtgttgcc atggtctgct gtacttcctc agccagtgac
    12961 gtatccatat catcctccac aaaatgataa aggctttaat aatgattatc atttatattt
    13021 tcggtcgcta acctaaaagc aaacgcgcac tatgtcaact cttgaggtaa cgcaatctgc
    13081 aactcggggt tgcatctggt tgtggttaca atgaaaatga gaagcattat tgatgaattc
    13141 gcataagcgc aatgtgatgt cctgcgccgt tttgccccct ctcccttcca gggtgagggc
    13201 tggggtgagg gttaatgttc gcaccagtgc tggctgttcc cctcacccta accctctccc
    13261 caaaggggcg aggggacgga ttgtgcgctt tgtcgaattt gtcattacgc ccttaacctt
    13321 attaataaca ggaagctgac ttgtgagact cgccccgctc taccgcaacg cccttctatt
    13381 aacaggactt ttgctttcag gaatagccgt agttcaggcc gccgactggc cgcgtcagat
    13441 tactgacagc cggggcactc ataccctgga aagccagccg cagcgtattg tttccaccag
    13501 cgtcaccctg accggatcac tgctggcgat tgatgctccg gtgatcgcca gcggcgcgac
    13561 cacgccgaat aaccgcgtcg cagatgacca gggcttttta cgccagtgga gcaaggtggc
    13621 gaaagaacgc aaactgcaac ggctctatat cggcgaaccg agcgccgaag ccgttgccgc
    13681 gcaaatgccg gatctgattt taattagcgc aaccggcggg gattcggtgc tggcgctgta
    13741 tgatcagctt tccaccatcg ccccgacatt aatcatcaat tacgacgaca aaagctggca
    13801 gtcgctgtta acgcagcttg gcgaaattac cgggcatgag aaacaagcgg cagagcggat
    13861 tgcacagttt gataagcaac tggcagcggc gaaagagcaa atcaaattac cgccgcagcc
    13921 ggtcactgcc attgtctata ccgccgctgc acacagtgcc aatctctgga cgccggaatc
    13981 agcacaaggg cagatgctgg aacaactcgg ctttacgctg gcgaagttgc ccgcagggtt
    14041 aaacgccagc caaagccagg gcaaacgcca tgacatcatt cagcttggtg gggaaaatct
    14101 ggctgctggg ttaaacggtg aatcgctatt cctgttcgcc ggtgatcaga aagacgccga
    14161 tgcgatttat gccaatccac tgctcgcgca cctgcctgca gtacaaaaca agcaggttta
    14221 tacgctggga accgagacgt tccgcctgga ttactacagc gccatgcaag tgctggacag
    14281 acttaattca ctgttaaaaa aagattgata aaacatacaa ttagagcact aataatcccc
    14341 tcgccccttt ggggagaggg ttagggtgag gggaaaatgc acctttagct aactgtcaga
    14401 cgctgtcacc tgcggcggcg tctggcgaaa acgtcgcaac tccaccagca ccagcaataa
    14461 caacacgccg ataatcaaca aaccaaaacc gctcgcgctt gcggaagcaa ccggtgtcat
    14521 catcgcgccc aaaccaccca acagcgccgc gcctatggca tcgcccgtca cgttctgcgc
    14581 cgtccacaaa ccgttaatcc gccctaacat cgcttccggg gtttgcgttt gcagcattgt
    14641 atattgcagc aacgaactaa ccgcgctcag ccagccgaac agcgccagac aaaccacgcc
    14701 taaaatccac atcggcatca ggccaaacag accaatggcg aggaacgatc ccagcgtgga
    14761 gagcagcatc aataaccctg gtcgcgcact atgtgccagc ttcccgctgg ttaacgcacc
    14821 aatagccgca ccgagcggga tcgccgcata gagaaaacca atctgtgccg ccgacatctg
    14881 ccagttgtca gccagcgccg gatacagtac ccgcaccgcg ctcgccatcg tcaataaacc
    14941 acccagcaac gcaatccctc ctaccagcgg gctggcgagc agaaaacgaa atcctgccag
    15001 taatgatttc aacggatgct cgcgcggctg cggtggcggt ggcaacgccg gaaggcttaa
    15061 caacggtagc aaggtaataa acgtgcccgc cgccgccagc ccgtagttcc aggctacgcc
    15121 gccggtcgcc agcaataaac cgccaatcat gggcgaaatc accgacccca gacgcacggt
    15181 caacatggtg atcgccccgg cctgcattaa gttttcacgc cctaccagtg caggtgttgc
    15241 cgccagtagc gccgtaacac caagtgatgc gaaaaaacca tcccataaac caagtaaata
    15301 gattgccagc aatgacggct ccggcagcag cgcgttcagg cacagtccaa tgaagccaat
    15361 gccacaggtg ccgcgcgcca gcaaaatcac ttttttgcgc tcatagcgat ccgccagcac
    15421 accgccaacc atcaggccaa caaacatcgc gccgccggtc agcgtcaccg aaagccccac
    15481 ctgccaggta gaatgcgtca tcatctggat ctgcaccggc accgcgacgc cgagcaaacc
    15541 cagagacaca attgagataa aacgagcgag gaatactgcg cgaaacgccg ggtgcgtttt
    15601 caacaggctg aggttaagca gccaggattg tttattcatt acaatgcctt gccatcaaat
    15661 attttaaata cctattctta ggctgcacat gctaacatat ccaaataaga tcgataacga
    15721 taattaattt cattatcatg gaagttcgta tgtctggttc tgttgccgtg acacgcgcca
    15781 ttgccgtgcc cggattgctg ttattactga ttatcgcgac ggcattaagc ctgctcattg
    15841 gggcaaaatc actccccgcg tcagtagtgc tggaggcctt ctcaggcacc tgccagagcg
    15901 ccgactgcac catcgtgctc gacgcgcgac tgccgcgcac ccttgccggt ttacttgcag
    15961 gcggtgcgct tgggcttgct ggggcgttaa tgcaaaccct cacccgaaat ccacttgccg
    16021 accccggctt gcttggcgtg aacgccggag ccagctttgc cattgtgctg ggtgcggcgc
    16081 tgtttggtta ctcatccgcg caggaacaac tggcgatggc cttcgccggg gcgctggtgg
    16141 cctcattgat tgttgccttt atcggcagtc agggcggcgg gcagttaagt ccggtgcgtt
    16201 taaccctggc gggcgtggcg ctggcggcgg tgctggaagg gctgaccagc ggcatcgccc
    16261 tgcttaatcc tgacgtctac gatcaactgc gtttctggca agccggttcg ctggatattc
    16321 gcaatctaca taccttaaaa gtggtgctga tcccggttct gatcgccgga gcaactgcgc
    16381 tattactgag tcgcgcgctg aacagtttga gcctcggcag tgacaccgcg acggcgctgg
    16441 gcagtcgcgt ggcgcgcaca cagttgattg gtctgctggc gattaccgtg ctttgtggta
    16501 gtgcgacggc ggtagttggc ccgattgcct ttatcggcct gatgatgccg cacatggcgc
    16561 gctggctggt aggtgccgat catcgctggt cgctgcccgt cacgctactt gctacccctg
    16621 ccctgctgct gtttgccgat atcatcgggc gggtgattgt gcccggcgaa ctgcgcgttt
    16681 ctgtggtcag tgcgtttatt ggtgcaccgg tgctgatctt cctcgtgcga cgtaaaacgc
    16741 gaggtggtgc atgatttacg tctctcgccg attactcatc acctgtttgc tgctgatttc
    16801 cgcctgtgtg gttgcaggta tctggggatt acgcagcggt gccgtcacgc tggaaatctc
    16861 gcaggtattc gccgcgttga tgggcgatgc gccgcgcagt atgacgatgg tggtcaccga
    16921 atggcgttta ccacgcgtgc tgatggcgct gttgattggc gcggcgctgg gcgtcagcgg
    16981 cgcgattttt cagtcgctga tgcgtaaccc gctcggcagc cctgacgtaa tgggctttaa
    17041 caccggggcg tggagcggcg tgctggtggc gatggtgctg tttggtcagg acctgacggc
    17101 tatcgcgctg gcagcaatgg tgggcggcat tgtcacttct ctgttggtct ggctgctcgc
    17161 ctggcgtaac ggtatcgaca cctttcggtt gattattatc ggcatcggcg ttcgcgccat
    17221 gctggtggcc tttaatactt ggctgttgct gaaagcgtct ttagaaacgg cgctaacagc
    17281 gggtttgtgg aatgccggat cgctcaatgg cctgacgtgg gcaaaaactt cgccttccgc
    17341 acccatcatc atattgatgc tcattgccgc cgccttgctg gtgcggcgaa tgcgcttgct
    17401 ggagatgggc gatgataccg cctgcgcgct gggcgtcagc gtcgagcgtt cgcgtctgtt
    17461 aatgatgctg gttgcagtgg tgcttaccgc tgcggcaacg gcgcttgccg ggccgatttc
    17521 ctttattgct ttagtcgcgc cgcacattgc ccgacgcatt agcggcaccg ctcgctgggg
    17581 gctgacccag gcggcgctgt gcggagcgct gttactgctg gcggccgatc tctgcgccca
    17641 acaactgttt atgccgtatc aacttccggt tggcgtcgtt accgtcagcc tcggcggtat
    17701 ttaccttatc gtcttgttaa ttcaggagtc tcgcaaaaaa tgaccgaatc agtagcccgt
    17761 ttgcgcggcg aacagttaac cctgggatat ggcaaatata ccgttgcgga aaatctgact
    17821 gtagaaattc ctgatggtca cttcacggca attatcggac caaatggctg tggtaaatcc
    17881 acgttactgc gtaccttaag ccgcctgatg acgcctgctc acgggcatgt ctggctggat
    17941 ggcgagcaca ttcaacatta cgccagtaaa gaagttgcac gccggattgg tctgttggcg
    18001 caaaatgcta ccacgccggg cgatatcacc gtgcaggagc tggtggcgcg tggacgttat
    18061 ccgcatcaac cgctgtttac ccgctggcgc aaagaggatg aagaagcggt aacgaaagcg
    18121 atgcaggcca cgagaataac tcatctggca gatcaaagcg tggataccct ttctggcgga
    18181 caacgccagc gagcgtggat cgcgatggtg ctagcccagg aaacggcaat tatgctgctc
    18241 gacgaaccca cgacctggct ggatatcagt catcagattg atctactgga actgttaagc
    18301 gaactgaacc gcgagaaagg ctataccctg gcggcggtgc tgcacgatct taatcaggcc
    18361 tgtcgttacg ccagccattt gattgcattg cgggaaggga aaattgttgc tcagggagcg
    18421 ccgaaggaga ttgtcactgc tgaactgatt gagcgcattt atggtctgcg atgcatgatc
    18481 attgacgatc cggtagccgg aacgccgctt gtggtgccgc tcggacgaac ggcaccttca
    18541 accgcaaata gttaaactaa gtggtctgcc atcatggcat cctgttttct ggatgccatc
    18601 gcatagcgca ataacacgct accacaagcc accatcccgc cgatcagcgc ggaaaggatc
    18661 acaataatcg ccttacccgg accgtctttt ttcactggca atgacgggct taactgatat
    18721 ttaaacggcg taaaattcac atcgttgaca tttgcttttg ttaattgctc gacaagatac
    18781 tgccgattac gtaattcacc gttcagttcc gcaacgtcag taaccgcttt ttcaatttcc
    18841 agtttgcgtt caataccgtc tgcgccgaga gaaatagaga aatcggggtc atctttaacg
    18901 gcctgaccat tactgtatac gggcttttta attcctgccg cgttggcaat gtcgagtgaa
    18961 taattgaggc gctgaatgtt tgcatcaagt tgatttttcg ttttaatgcg atcctgagcc
    19021 agtttttctt tttcaaactg ggttttgatc tccagtttat tacggacgtt ttctatcgac
    19081 tctttcacca ccaacgcaga gatataatcg atatacccgc tcaaaacggt ctgcgcctct
    19141 ttactggttg gcgcggtaaa acttagcgtc caggaggtat acagtgacgg ttcatctttt
    19201 ttcttactgg cattgtcatc aaccgctttc attttttcgc tcaatgcaac aatagcgcga
    19261 tgcaaatcca gttcatcgat tttcgcctct tttaattggt ccatcacata aggtgatgaa
    19321 cgcaggtact cttccagcaa gctaacagac tgaaacttct tgataaacag gttaaatgca
    19381 ccgccacggt cgatattaac atcgagatca agaacgcgga gtttggtaaa ggttttctcc
    19441 aggtcctgcc actgaatggc ttcggcggga gtaataacgg ctgagctggt ccatttttgc
    19501 ggcaggatga aagagatcag taaacctgcg caggcaaatg caaaaacgac cgccatgacc
    19561 gtttttttgg cccgccataa aacttcgatt agattaagta aatcaatttc attattactg
    19621 ggcgacgcca gaggatactc agaaaaatga gcgtcacttc cctgtttaat attcaatgat
    19681 gacatggggt ataacctgaa aatgagtcca ataggggaaa atctccacgg cgctcacttt
    19741 accagttggt gagaattatc cgaagctgaa gtttgtaaat tccttccact gaatgc
//
LOCUS       DAZPDU010000072        19767 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZPDU010000072 DAZPDU010000000
VERSION     DAZPDU010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19767
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.073"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            215..985
                     /locus_tag="ACHURS_002303"
     CDS             215..985
                     /locus_tag="ACHURS_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFR1238019.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            976..1449
                     /locus_tag="ACHURS_002304"
     CDS             976..1449
                     /locus_tag="ACHURS_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFR1238020.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1557..2096
                     /gene="dnaT"
                     /locus_tag="ACHURS_002305"
     CDS             1557..2096
                     /gene="dnaT"
                     /locus_tag="ACHURS_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFR1238021.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2099..2836
                     /gene="dnaC"
                     /locus_tag="ACHURS_002306"
     CDS             2099..2836
                     /gene="dnaC"
                     /locus_tag="ACHURS_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFR1238022.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2885..3379
                     /gene="yjjA"
                     /locus_tag="ACHURS_002307"
     CDS             2885..3379
                     /gene="yjjA"
                     /locus_tag="ACHURS_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFR1238023.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3633..5924
                     /gene="opgB"
                     /locus_tag="ACHURS_002308"
     CDS             3633..5924
                     /gene="opgB"
                     /locus_tag="ACHURS_002308"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFR1238024.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6063..7085)
                     /gene="lgoD"
                     /locus_tag="ACHURS_002309"
     CDS             complement(6063..7085)
                     /gene="lgoD"
                     /locus_tag="ACHURS_002309"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HFR1238025.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7224..8138
                     /locus_tag="ACHURS_002310"
     CDS             7224..8138
                     /locus_tag="ACHURS_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFR1238026.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIHSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8353..9714
                     /locus_tag="ACHURS_002311"
     CDS             8353..9714
                     /locus_tag="ACHURS_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1238027.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHNFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLVVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9763..11418)
                     /gene="tsr"
                     /locus_tag="ACHURS_002312"
                     /pseudo
     CDS             complement(9763..11418)
                     /gene="tsr"
                     /locus_tag="ACHURS_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418775.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
     gene            complement(11537..11983)
                     /gene="hpaR"
                     /locus_tag="ACHURS_002313"
     CDS             complement(11537..11983)
                     /gene="hpaR"
                     /locus_tag="ACHURS_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HFR1238028.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
     gene            12255..13544
                     /gene="hpaG"
                     /locus_tag="ACHURS_002314"
     CDS             12255..13544
                     /gene="hpaG"
                     /locus_tag="ACHURS_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HFR1238029.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTHKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEDTAK"
     gene            13541..15007
                     /gene="hpaE"
                     /locus_tag="ACHURS_002315"
     CDS             13541..15007
                     /gene="hpaE"
                     /locus_tag="ACHURS_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HFR1238030.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADSLGELALEADIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15009..15860
                     /gene="hpaD"
                     /locus_tag="ACHURS_002316"
     CDS             15009..15860
                     /gene="hpaD"
                     /locus_tag="ACHURS_002316"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HFR1238031.1"
                     /translation="MGKLALAAKITHVPSMYHSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            15870..16250
                     /locus_tag="ACHURS_002317"
     CDS             15870..16250
                     /locus_tag="ACHURS_002317"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HFR1238032.1"
                     /translation="MPHFIVKCSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            16361..17164
                     /gene="hpaH"
                     /locus_tag="ACHURS_002318"
     CDS             16361..17164
                     /gene="hpaH"
                     /locus_tag="ACHURS_002318"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HFR1238033.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17175..17963
                     /gene="hpaI"
                     /locus_tag="ACHURS_002319"
     CDS             17175..17963
                     /gene="hpaI"
                     /locus_tag="ACHURS_002319"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HFR1238034.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HTPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAVKPGVY"
     gene            18138..19514
                     /gene="hpaX"
                     /locus_tag="ACHURS_002320"
     CDS             18138..19514
                     /gene="hpaX"
                     /locus_tag="ACHURS_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HFR1238035.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLSVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGMLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATL"
     gene            19524..>19767
                     /locus_tag="ACHURS_002321"
     CDS             19524..>19767
                     /locus_tag="ACHURS_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HFR1238036.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPS"
BASE COUNT         4707 a         5338 c         5111 g         4611 t
ORIGIN      
        1 gccaaacaat aaattcatta tagtgataat attcatgtga tagtttgttt gcggcgagag
       61 ataattcgct ttttatcacc gagcttgtcc cgataaccag gattaatcag cgcaattttg
      121 atcttaacct accgcaaaac aaccgtgctt catgatacaa atggattctc attatctgaa
      181 caatacgcag cacgtctatg acaaagggcg agttatgcaa actgagcaac agcgagccgt
      241 aacacggctt tgtatccagt gtggattatt tcttttgcaa catggtgcgg aaagcgcgtt
      301 ggttgatgag ctttcctcac gactgggtcg ggcgttgggg atggacagcg tcgaaagttc
      361 tatctcttcg aacgccatag tgctgacaac cattaaagat gggcaatgcc tgacatcgac
      421 acgtaaaaat cacgatcgcg gcattaatat gcatgtggtg actgaagtcc agcacattgt
      481 gattctggcg gagcaccatc tgctggatta caaaggcgta gagaaacgat ttagccaaat
      541 tcagccatta cgttacccaa gatggctggt agccttaatg gttggccttt cttgcgcctg
      601 tttctgcaaa ctcaataaag gtggctggga tggtgccgtc atcaccttct ttgccagtac
      661 ggccgcgatg tatatccgcc agctgctggc acaacgtcat cttcatccgc agatcaactt
      721 ttgcctcacc gctttcgccg ccaccaccat ttccggattg cttttgcaac ttcccacttt
      781 cagcaataca cccactatcg cgatggccgc cagcgttctg ctgctggtgc cgggatttcc
      841 gttgattaat gccgtcgccg atatgtttaa aggccacatc aataccggac tggcacgctg
      901 ggcgatcgcc agtctgctga cactggctac ctgcgtcggc gtggtgatgg cactgacgat
      961 ttgggggcta cgcggatggg tgtgatcgag tttctgtttg cattggcgca ggatatgatc
     1021 ctcgccgcca ttccagcggt cggctttgcg atggtgttca acgttcccgt tcgggcatta
     1081 cgctggtgtg cgctgcttgg cgcgattggt catggttcac gaatgatctt aatgaccagc
     1141 gggctgaata ttgagtggtc aacctttatg gcctccatgc tggtcggcac cattggtatt
     1201 caatggtcgc gctggtatct ggcgcatccg aaagtgttta ccgtggcggc cgttatccct
     1261 atgttcccgg gcatatcggc ttataccgca atgatttcgg cggtaaaaat cagccagtta
     1321 ggttacagcg aaccgttgat gattaccctg ttaaccaact ttcttacagc ttcatcgatt
     1381 gttggtgcgt tatccatcgg tctttccatt cctggattat ggttgtaccg caagcgccct
     1441 cgcgtataaa attgcctcgc ttaaagcaca cggatgagag acagcctcct ctcctccgtg
     1501 tgttactata aaagttatct cccttctcgt tcatcgttcc atatttgaga aacagtatgt
     1561 cttccagagt tttgaccccg gacgtcgttg gtattgacgc cctggtacac gatcaccaaa
     1621 ccgttctggc aaaagctgaa ggcggtgtgg ttgccgtatt tgctaacaat gccccggcgt
     1681 tttatgccgt cacgcctgca cgcctggctg aactgctggc gctggaagaa aagctggcgc
     1741 gtccgggaag cgatgtcgct ctggacgatc aactctatca ggaaccgcaa gccgctcccg
     1801 ttgctgtacc catggggaaa ttcgccatgt atccggactg gcaacccgat gccgatttta
     1861 tccgcctggc ggcgctatgg ggtgtggcac taagagagcc ggtgaccacc gaagaactgg
     1921 cctcattcat tgcctactgg caggcggaag gtaaagtctt tcaccatgtg cagtggcaac
     1981 aaaaactggc gcgcagcctg caaatcggtc gtgccagcaa cggcggactg ccgaaacgag
     2041 atgtgaatac ggtcagcgaa cctgacagtc aaattccacc aggattcaga gggtaacgat
     2101 gaaaaacgtt ggcgacctga tgcaacgcct gcaaaaaatg atgcctgccc atatcaaacc
     2161 tgcattcaaa acgggtgaag aactactggc ctggcagaaa gaacaaggag cgatccgctc
     2221 cgccgctctc gaacgtgaaa atcgggcgat gaaaatgcag cgcaccttta accgctccgg
     2281 tattcggcca ctgcatcaga actgctcctt tgagaactat cgcgttgagt gtgaagggca
     2341 gatgaatgcg ttaagcaaag cgcgccagta cgtcgaagag tttgacggca acatcgccag
     2401 cttcatcttt tctggtaagc caggaaccgg caaaaaccat ctggcggcgg caatctgcaa
     2461 cgagctgctg ctacgcggta aatccgtatt gatcatcacc gtggccgata ttatgtcggc
     2521 gatgaaagat accttcagga atagcggtac cagtgaagaa caactgctta acgatctgag
     2581 caacgttgat ctgctggtga tcgatgagat cggcgtgcag accgaatcga aatacgaaaa
     2641 agtgatcatc aaccagatcg tcgatcgccg ctcttcttcc aaacgcccaa ccgggatgct
     2701 gaccaacagt aatatggaag agatgaccaa gttgctgggc gaacgcgtga tggaccgtat
     2761 gcgcctgggt aacagtttgt gggtgatctt caactgggat agctaccgta gccgggtaac
     2821 aggtaaagag tattaatccc cgtttctggc ggtatactgc gacacaattc caccttgagt
     2881 aatgatgatg aaaactgtta aacatcttct gtgctgtgcc attgccgcca gcgcattaat
     2941 ttccaccggg gtgcatgctg cgtcctggaa agatgcgctc tccagcgccg ccagcgaact
     3001 tggcaaccaa aacagcacga cacaggaagg cggttggtcg ctcgcgtcat taactaactt
     3061 gcttagcagc gggaaccagg ccttaagcgc agataacatg aacaacgccg caggcattct
     3121 gcaatactgc gcgaagcaaa agctggcgtc ggtaaccgat gccgaaaaca tcaagaacca
     3181 ggtgctggaa aagctgggcc tgaacagtga agagcaaaaa gaagacacca actatctgga
     3241 tggcattcag ggtttgctga aaaccaaaga tggtcagcaa ctcaatctgg ataacatcgg
     3301 aacgactccg ctggcagaaa aggtgaaaac caaagcctgc gatctggtgt taaagcaggg
     3361 gctgaacttc atttcctgaa ccgcgaaacg ccgcgtttta gctgttaccc ggctgacgcg
     3421 gctctctcct gcccttttcc ctgctcctgg acggttttcc ccctgttaca cgggaagcga
     3481 ccgcgatcaa aaaagtctgt ttataaacta attcctaaca acatcgcatt tttttgacac
     3541 tagaattgca gcaatatggc gacgccatta cattgtgctt ctcaaaaagc ttaaacgttg
     3601 gctaaacggc ccggtttagt gaggatcgcc cgttgtcaga actactctct ttcgcccttt
     3661 ttctcgcctc tgtgctgatt tacgcatgga aagcgggacg taacacctgg tggtttgcag
     3721 ccacattaac ggtgctgggg ctatttgtcg ttttaaatat caccctgttt gccagcgact
     3781 attttactgg cgatggtatt aacgacgcgg ttctctatac cttaaccaac agcctgaccg
     3841 gtgctggcgt cagcaaatac atcctgccgg gtatcggcat tgtgctgggg ctgacagcgg
     3901 tgttcggtgc gctgggctgg atcctgcgcc gtcgtcgcca tcatccgcac cattttggtt
     3961 acagcctgct ggcgctctta ctggcgctgg gttcggtgga cgccagcccg gcatttcgtc
     4021 agataacgga actggtgaaa tcccagtcac gcgatggcga cccggacttt gcggcttatt
     4081 ataaagagcc gtcgaaaact atccctgacc cgaaactcaa cctggtttat atctacggcg
     4141 aaagtctcga gcggacctat tttgataacg aagctttccc ggatctcacg cctgaactgg
     4201 gcgcgttgaa aaatgaaggc ctggatttca gccacacgca gcagctgcca ggaacggatt
     4261 acacgattgc gggcatggtg gcttctcagt gcggcatacc gctgtttgcc ccctttgaag
     4321 gcaacgcctc cgcctctgtc tccagcttct tcccgcagaa catctgtctg ggcgatatcc
     4381 tgaaaaactc gggttatcag aactatttcg tgcagggcgc gaatctgcgt tttgccggta
     4441 aagatgtgtt cctgaagtcg catggcttcg accacttata cggctcagaa gagctgaaaa
     4501 gcgtggtggc cgacccgcac tatcgcaacg actggggatt ctacgacgat accgttctcg
     4561 atgaagcgtg gaaaaagttt gaagagcttt cccgctcagg tcagcgattc tcactgttta
     4621 ccctgacagt cgatacccat cacccggatg gttttatctc gcgtacctgt aaccgcaaaa
     4681 aatatgattt tgacggtaag ccgaaccagt cattcagcgc ggtaagttgc agccaggaga
     4741 atatcgcggc gtttatcaac aaaatcaaag cgtcaccgtg gtttaaagat accgtcatcg
     4801 tcgtctcttc tgaccattta gcgatgaaca acacggcgtg gaaatacctc aataagcagg
     4861 atcgcaataa cctgtttttt gtcattcgtg gcgacaagcc gcagcaagag acgctggcag
     4921 tgaagcgtaa cacgatggat aacggcgcga cggtgctgga cattctcggt ggcgataact
     4981 atctcggact tggtcgtagc agtttatccg ggcagtcgat gtcggaaatc ttcctcaata
     5041 tcaaagagaa aacattggcg tggaagccgg atatcatccg cctgtggaaa ttccctaaag
     5101 agatgaaaga gttcaccatc gaccagcaga aaaacatgat tgccttctcg ggtagccatt
     5161 tccgtttgcc gctgctgttg cgggtttcag acaaacgcgt ggaaccgctg ccggaaagcg
     5221 aatactcagc accgctgcgt ttccagctgg ccgatttcgc tccacgcgac aatttcgtct
     5281 gggttgaccg ttgctataag atggcacaac tctgggctcc ggaactggca ctctccaccg
     5341 actggtgtgt ctcgcaaggg cagcttggcg gtcagcaaat tgttcagcat gttgacaaaa
     5401 caacatggca gggcaaaacg gcatttaaag atacggtcat cgacatggcg cgttataaag
     5461 gcaatgtcga tacgctgaaa attgttgata acgatattcg ctacaaagcc gacagtttca
     5521 tcttcaacgt cgccggtgcg ccagaagagg tgaaacagtt tagtgggatt tcccgtccag
     5581 agtcgtgggg ccgctggtcc aacgcgcagc tgggcgatga agtaaaaatc gagtacaagc
     5641 atccgctgcc aaagaaattt gacctggtga ttaccgccaa agcatacggc aataacgcca
     5701 gccgtcctat tccggtacgc gtaggcaatg aagaacaaac ccttgtgctg ggcaatgaag
     5761 tgaccaccac cacactgcat ttcgataacc caaccgatgc cgacacactg gtaattgtgc
     5821 cgccggaacc tgtctcaacc aacgaaggga atatcctcgg acactcgccg cgtaagctcg
     5881 ggatcggcat ggtggaaatt aaagtggtgg aacgtgaagg ataattcctg actgatgtgt
     5941 aggtcgcata aggtgcgacc tacacacctt ctgcggccta caaaacatca ttgagaaaca
     6001 gaaaaaaaca gcgcccaaca cttccccgga cgctgcatgg caactttaat aatctcgcct
     6061 tatcagaaag taattacacc cttaatcaac tcacgattgt taatcacatc gcgctcgtag
     6121 gtttctgcca gcgtggcgaa cggatagcga tgggttaaca tcatgtcagc agtgattttt
     6181 ccttccgcca tcagtcgacc gactttagca aaatcttccg gcgtggcgtt gcggctaccc
     6241 atcatcgtcg tttctttttt atggaattcc ggatcggaga actgcaactc acctttaaac
     6301 aggccgacaa ataccaccgt gccgccgtga cgaatcaaat tcacggtgtt attcatcgca
     6361 tgttgattac ctgtcgcgtc gatcactttc tgcgccagcg aaccaccaaa ctgcgcccgt
     6421 agctgcgcgt caaaatcttc ggctgacggg tccagtaaag gtaattccag acgcgttgcc
     6481 acatgttcac ggcgcgccgg actggtatcc gccaccacca cctgtgcgcc atcggcttta
     6541 gcgattgccg ccgcgcccag accgattggc cccgccccga ccaccagcac ctgctcgccg
     6601 ggagcaatgg ctgcgcgacg caccgcatga gcgctaatag cgaaaggttc aatcaatgct
     6661 gccgcctgcg ggtcaatacc gtctgcgggc aaaatgttcg ccaccggcac gctcaaatac
     6721 tcactaaaac cgccatcctg atgcacgcca atgactgaaa ttttttcaca gcaattggta
     6781 cgcccgcttt tacacgccgg gcattgctga caggcaacat aagggatcac agcaacttgc
     6841 tgaccatttt taagatcagc aatattttta cccagcccaa caatctcccc acatatttca
     6901 tggcctaaaa cacgtggata actaaaaaat ggttgatttc caccccaggc atgaatatcg
     6961 gtaccgcaaa tcccgacaga cttaattttt attaatgctt cattgtcacc cggaataggt
     7021 atctcgcgtt gtttccagac taattctttc ggctgctggc aaattaaaac attcatcgta
     7081 gacatgattc ggctctccat tttttgttgc cttagatatc aatgaaaatg aaaaatggca
     7141 gcggaacgga aaatctgttt tgtgaaaaca cacgcataaa aatgttttaa atcgggtttt
     7201 aatggactca caaggaggaa aatatgagtc gttcacaaaa tttacgccac aatgtgatta
     7261 accaggtcat tgatgatatg gcgcggggtc atatcccttc cccacttcca tcgcaaagtg
     7321 cgctggcgga aatgtacaac atcagccgca ccacagtgcg tcatattctc agccacttac
     7381 gcgaatgcgg cgtcttgacg caggttggaa acgactatgt tattgcgcgt aaacctgacc
     7441 atgatgacgg tttcgcctgt actaccgctt caatgagtga acaaaacaaa gtgtttgagc
     7501 aggctttttt cacgatgata aaccagcgcc agttacgccc aggggaaacg ttttctgaac
     7561 tgcaactggc tcgggccgcg ggtgtcagcc cggttgtagt aagagaatat cttttaaaat
     7621 tcggccgtta taatcttatt cacagcgaaa agcgcggcca gtggagcatg aagcaattcg
     7681 atcaatccta cgccgagcag ctgtttgagc tacgcgaaat gctcgaaaca cattcacttc
     7741 agcactttct caacctgcct gatcatgatc cccgctggct gcaggcgaaa accatgctcg
     7801 aacgtcatcg gctactgcgt gacaacatcg gcaatagctt tcgcatgttc tcgcaacttg
     7861 acagagattt tcactcctta ctgctttcag ctgccgacaa tatctttttt gatcaatcgc
     7921 ttgagatcat ctcagtcatc tttcattttc actatcaatg ggacgaaagc gatcttaaac
     7981 agcgcaatat cattgccgtc gatgagcata tgaccattct cagcgctctg atttgccgca
     8041 gtgaccttga tgccaccctt gcgctccgta accatttgaa ttcagctaaa caatcaatga
     8101 ttcgatccat taatgagaac acgcgttatg ctcattaagt acggattaaa aacagcaaac
     8161 tattagcggg tgcacggttt tatcaaaaga taaataccgg gaaattcccg cttacctatg
     8221 ctcacaatca gtcgactaaa aatgtttttg tgagcggtag taaagtccta aaactttaac
     8281 ctgccgttaa tgtcaacctt gcgtaattca caattacgta aacaattaaa attataaata
     8341 caggagtccg gcgtggaaaa agaaaatatc accctcgatc cgcgttcttc atttactcca
     8401 tcttcgtcgg cagatattcc cgtgccacca gatggattag ttcaacgcag tacccgaatt
     8461 aaacgcattc aaaccaccgc catgttgtta ttattttttg cggcggtaat caattatctc
     8521 gaccgcagtt cgctgtcggt agcaaattta acgattcgtg aagaattggg attaagtgcc
     8581 accgaaatcg gcgctttgct ctccgtgttt tcactcgctt acgggattgc gcaacttcct
     8641 tgcggcccac tattggatcg taaaggccca cgcctgatgc tgggactggg gatgttcttc
     8701 tggtcactgt tccaggcaat gtctggcatg gtgcacaact ttacgcagtt cgtgttggtg
     8761 cgtatcggta tggggattgg tgaagcgccg atgaacccat gcggtgtaaa agtcattaac
     8821 gactggttca acatcaaaga gcgcggacgc ccgatgggct tcttcaacgc agcttctacc
     8881 attggcgttg ccgtaagccc accgattctg gcggcgatga tgctggtgat gggctggcgc
     8941 gggatgttta ttaccattgg tgtactgggg atttttctcg ccatcggctg gtatatgctc
     9001 tatcgcaacc gcgagcacgt agaactgact gccgttgaac aagcttatct caatgcaggt
     9061 agcgtcaatg cccgccgaga tccgctcagt tttgccgaat ggcgcagcct gttccgtaac
     9121 cgtacaatgt ggggaatgat gctcggattc agtggcatca actacactgc gtggctgtat
     9181 ctggcctggc ttcctggtta cctgcaaaca gcctataacc tggatttaaa aagcacaggg
     9241 ttgatggcgg ctatcccttt cctgtttggg gctgccggga tgctggtcaa cggttacgtt
     9301 actgactggc tggtcaaagg gggaatggct ccgattaaaa gccgtaagat ctgcattatt
     9361 gccgggatgt tctgttctgc cgcctttacg ctggtcgtac cgcaagcgac aacatccatg
     9421 acagcggttc tgctgattgg catggcactg ttctgtattc actttgccgg aacatcctgc
     9481 tggggcttga tccacgtcgc agttgcttct cgcatgactg cgtcggtggg cagtatccag
     9541 aactttgcca gcttcatctg cgcctctttt gcgccgatca ttactggttt tattgttgat
     9601 accacccact cattccgtct ggcactaatc atctgcggtt gcgtcaccgc agcgggggca
     9661 ctggcgtaca tcttcctggt tcgtcagccg atcaacgacc cacgtaaaga ttaatctcct
     9721 tatgcccgat aacattttgc ttatcgggca ttttcatggc gattaaaatg tttcccagtt
     9781 ctcctcgcta tctgccacgg ccattttacg cggcgcagct ggcgtcacgg tttttaccac
     9841 agccgatgtt tcacgctgct gttgctgaat ccggaacact gccactgctt cggtcaggcg
     9901 actggcctgc tcttccagcg cggcggcggc agcggcagac tcttccacca gcgcggcgtt
     9961 ctgttgagtt acccggtcca tctcagcaac cgctaagcca acctgatcga taccacggct
    10021 ctgctcatca gaagcagaag caatttcgcc cataatgtcc gtcacgcggg tcacggcgct
    10081 gacaatctcc gccattgttt ccccggcgct ttcgaccagc gtagagccaa catccacttt
    10141 ccccaccgag tcttcaatca ggcttttaat ttcacgagcc gcctgggcgc tgcgctgggc
    10201 cagattacgc acttctcccg cgaccaccgc aaaaccgcgc ccttgctcac ccgcacgcgc
    10261 agcctcaacc gccgcgttca aagccagaat attggtctgg aaggcaatgc cgtcaattac
    10321 gctgataata tcggcgattt tctgcgaact ggtggagata tcgcgcatag tctgcaccac
    10381 gttatctacc actttgccgc cgcgttgcgc cgtttcagaa gcacttaacg ccagatggct
    10441 ggcctggcgc gcattttcgg cgttctgctt caccgttgcg gtcagttact ccatgctggc
    10501 tgccgtctct tccagcgaag cggcctgttg ctcggtgcgc gaagagagat cgttattgcc
    10561 ggtggcgatt tcgctggcac cgctatagat ggcattggcc ccgttgcgca catcaccgac
    10621 ggtacgcatc agctctccct gcatatggcg caaactctct gccagttgcc ccatctcatt
    10681 agagccatcc acctcaatcg gtttcaccag atcgccgcct gcaatatgac gaatgctgtc
    10741 aatcaggcga ttcattggcg ctaccagcga ggctttaata ccgaaccaga cggcgaagat
    10801 gaccgccagt acgacgatca tcacgcccac cagaatccac atcgcctggc tgtaggaggc
    10861 attgttatcg ctgacggcga tatcatagag ccgatcgttt tgctccatgt aagccacata
    10921 ctgcttctcg aaaccgtcct gatatccctg ggtcggctga tcaaagaact cgttgatttt
    10981 gcctgcacct aacagttgga tcagctccgc cagcgcattg tgataaatat cgtaattacg
    11041 tttgatctct gccgctgcgg cggtgctctg acgcgggtca cgcggcaacg cttcgtaatc
    11101 cgcccagttt ttttccgcct gtttcagcga aatactggca ctctgcatca gctcagcaac
    11161 ggttgaaccg ctaccaatat tgttctgatc catcatgtag cggatacccg cgcggttgag
    11221 ggtgttacgc gtctgcaaca acgcgaccca gctgccattc agcgtggatt gctgctggcg
    11281 aatggtttgt aaaacagtga aattttcttt gtcattcttt aaggcattaa agaacagacc
    11341 gcctgatgtc agttgtaaaa ggccaaaaac ggccaaaacc agcagtaagc tggtcacgat
    11401 cttgatacgt tttaacatgg tttctctttc ctgtggaccg atgcaagatt ttcggcctgg
    11461 aaagcaaaaa ctttatgaaa aggtgtaacc caccatccat ggaaaaacca ggcgctggtt
    11521 atcagatact aaaaagttat tcattatcgc ccgggatggc ttcttgtcgg gaattgccca
    11581 gagcaataaa ttcctctaac agatgggtta actgttgcat tttctcggca gtaaactgag
    11641 cttcaatctg ccgataggct tcttcaatct gtgtctgagc gcggttatac agcgcctgcc
    11701 cctctttggt tagcgagata tagagcttcc gctgatcatt gatcggcttt aatcgcaaca
    11761 ctaaaccgtc gcgttccatc cgcgtgagga tcccggtcag gcttgggcgc aaaatgcagg
    11821 cgcgatacgc cagatcgtga aaatccattg atgggctttc cgccagaata cgcacgatgc
    11881 gccactgttg ctcagtcaaa ttatgccgtt taacgattgg gcgaaaataa ctcatcgccg
    11941 cttctcgcgc ctggagcaac gcaatggtta gtgagtcgtg cattatcttt cccctgaaaa
    12001 attcaaaatc attaatatag aaacagttaa tcgtcacgtt accgggactg cgctgcccct
    12061 gcaattcctg attttatcaa aattgatgat attttattaa cttactgata tttaatattt
    12121 tattatcttt aaatgtaaat agtttgttaa ttagatcaca tttacatcac ttacttttgc
    12181 caaactacgc aatcgggaat aaaagcaatc attaacatat taatgattaa gatgatcgaa
    12241 tccgaggagt ggtaatgaaa ggcactatct tcgccgtagc gttgaaccat cgcagccaac
    12301 ttgatgcatg gcaggaagcg ttccagcaat ccccctacaa agccccgcct aaaactgcgg
    12361 tctggtttat taaaccgcgc aatacggtga ttggttgcgg tgaaccgatt ccctttccac
    12421 agggtgaaaa ggtactgagc ggtgcgactg ttgcgctgat tgtgggaaaa acggcgacga
    12481 aagtacgtga agaagatgcg gcagagtaca tcgccggata tgcgctggct aacgacgtca
    12541 gcctgccgga agagagcttt taccgcccgg caatcaaagc aaaatgtcgt gatggattct
    12601 gccccattgg cgaaaccgtg gctctcagca atgtcgataa tctgaccatc tataccgaga
    12661 tcaacgggcg tcctgccgat cactggaata ccgccgattt acaacgtaac gccgcgcagt
    12721 tgctgagcgc cctgagcgaa tttgccacgc tgaatccagg cgacgccatt ctgctcggca
    12781 cgccacaggc gcgcgtggaa atacagccag gcgatcgcgt tcgtgttctc gcagaaggtt
    12841 tcccgccgct ggaaaatccg gtagtggacg aacgtgaagt gaccacgcac aagagcttcc
    12901 caacgcagcc acacccgcac ggcacgctgt ttgccctcgg cctgaactac gccgaccacg
    12961 ccagcgaact ggaatttaag ccaccggaag aaccgctggt gttcctgaaa gcgccaaata
    13021 ccctcactgg cgataaccag acctccgtgc gtccaaacaa tattgaatac atgcactatg
    13081 aagcggagct ggtggtagtt attggcaaac aggcgcgtaa cgtcagcgaa gccgatgcca
    13141 tggattatgt cgcgggctac accgtgtgta acgactacgc cattcgtgac tatctggaaa
    13201 actactaccg ccctaacctg cgggtaaaaa gccgcgacgg actgacgccg atgctttcaa
    13261 ccatcgtgcc gaaagaggcg atcccggacc cgcataatct gacccttcgc accttcgtca
    13321 acggcgagtt acgccagcaa ggcaccaccg ccgatctgat cttcagcgtg cccttcctga
    13381 tcgcctactt aagtgaattt atgaccctga atccgggcga catgatcgcc accggcacac
    13441 ccaaaggctt atctgacgtg gtgcctggcg atgaagtagt ggtggaagta gaaggcgtgg
    13501 gccgcctggt gaaccgaatt gtgagtgagg atacagcgaa atgaaaaaag taaatcattg
    13561 gatcaacggc aaaaatgttg caggtaacga ctacttccag accaccaatc cggcaacggg
    13621 tgaagtgctg gcggatgtgg cctctggcgg tgaagcggag atcaatcagg cggtagcggc
    13681 agcgaaagag gcgttcccga aatgggccaa tctgccgatg aaagagcgtg cgcgcctgat
    13741 gcgccgtctg ggcgatctga tcgaccagaa cgtgccagag atcgccgcga tggaaaccgc
    13801 ggacaccggc ctgccgatcc atcagaccaa aaatgtgttg atcccacgcg cttcccacaa
    13861 ctttgaattt ttcgcggaag tctgccagca gatgaacggc aagacctatc cggttgacga
    13921 caagatgctc aactacacgc tggtgcagcc ggtgggcgtt tgtgcgctgg tatcgccgtg
    13981 gaacgtaccg tttatgaccg ccacatggaa ggtcgcgccg tgtctggcgc tgggcaatac
    14041 cgcggtactg aaaatgtcgg aactctcccc gctgaccgct gacagcctgg gtgagctggc
    14101 gctggaagcc gatattccgg caggcgtgct gaacgtggta cagggctacg gcgcaaccgc
    14161 aggggatgcg ctggttcgtc atcatgacgt acgtgccgtg tcgttcaccg gcggtacggc
    14221 caccgggcgc aacatcatga aaaacgccgg gctgaaaaaa tactccatgg aactgggcgg
    14281 taaatcgccg gtgctgattt ttgaagatgc cgatattgaa cgcgcgctgg acgccgccct
    14341 gttcaccatc ttctcgatca acggcgaacg ctgcaccgcc ggttcgcgca tctttattca
    14401 gcaaagcatc tacccggaat tcgttaaacg ctttgccgaa cgcgccaacc gtctgcgcgt
    14461 gggcgatccg aacgatccga atacccaggt tggcgcgctt attagccagc agcactggga
    14521 aaaagtctcc ggctatatcc gtctcggcat tgaagaaggc gcaaccctgc tggcgggcgg
    14581 cccggataaa ccgtccgacc tgcctgcgca cctgaaaggc ggcaacttcc tgcgcccaac
    14641 cgtgctggca gacgttgata accgtatgcg agtcgcccag gaagagattt tcgggccggt
    14701 cgcctgcctg ctgccgttta aagacgaagc cgaaggctta cgtctggcaa acgacgtgga
    14761 gtacggcctc gcgtcgtaca tctggacaca ggatgtcagc aaagtgttac gcctggcgcg
    14821 tggcattgaa gctggcatgg tgttcgtcaa cacccagaac gtgcgtgacc tgcgccagcc
    14881 atttggcggc gtaaaagcct ccggcaccgg gcgtgaaggc ggtgagtaca gcttcgaagt
    14941 gttcgcggaa atgaagaacg tctgcatttc catgggcgac catccaattc cgaaatgggg
    15001 agtctgatat gggtaagtta gcgttagccg ccaagatcac gcacgtaccg tcgatgtatc
    15061 actctgaact gccagggaaa aaccacggtt gccgccaggg cgcgatcgac gggcataaag
    15121 agatcagcaa gcgttgccgg gaaatgggcg tcgataccat tatcgttttc gatacccact
    15181 ggctggtcaa cagtgcttat cacatcaact gtgcagacca ttttgaaggc gtctacacca
    15241 gcaacgagct gccgcatttt atccgcgata tgacctacaa ctacgagggc aacccggagt
    15301 tggggcagct tattgccgat gaagccttaa agctcggcgt gcgggcaaaa gcgcacaaca
    15361 ttcccagcct gaaactggaa tacggcacgc tggtgccgat gcgctacatg aatgaagaca
    15421 agcacttcaa agtggtctcc atttcggctt tctgcacggt tcacgatttt gccgacagcc
    15481 gcaagctggg cgaagcgatt ctgaaagcga tcgaacagta cgacggcacc gtggcggtcc
    15541 ttgccagcgg ttcgttatcg caccgcttta ttgacgatca gcgtgcggaa gaagggatga
    15601 acagctacac ccgcgagttc gaccgccaga tggacgagcg cgtggtgaag ttgtggcgcg
    15661 aaggccagtt caaagagttc tgcaatatgc tgccggagta cgccgactac tgctacggcg
    15721 aaggcaatat gcacgacacg gtgatgctgc tggggatgct cggctgggat aaatacgacg
    15781 gcaaggtgga gtttatcacc gagctattcc caagctctgg caccggtcag gttaacgctg
    15841 ttttcccgct tcccgcgtaa ggacgtttta tgccgcactt tatcgttaaa tgcagtgaca
    15901 acatccgcga agaagccgac ctgccggggt tgttcgccaa agtgaatccg acgctggcag
    15961 ccacgggtat ttttccgctg gcgggtattc gcagccgcgt gcattgggtc gatacctggc
    16021 agatggccga cgggcagcgt gattatgcct tcgtgcatat gacgttgaaa atcggcgcag
    16081 gtcgcagcct ggaaagccgc cagcaggcgg gtgaaatgct gtttgaactg attaaaacgc
    16141 acttcgccgc cctgatggag agccgcctgc tggcgttgtc gtttgagatt gaagagctgc
    16201 atccgacgct gaattttaaa caaaacaacg tgcacgcatt gtttaagtag cgcgcagatt
    16261 gcccggtggc gctgcgctta ccgggcctac aaaaccccaa accgtacacc gtaggccgga
    16321 taaggcgcag ccgcatccgg caatgccaca ggatatcgct atgttcgata aacacaccca
    16381 caccctgatc gcccagcgtc tggatcaggc agaaaaacag cgcgaacaga tccgcgcgat
    16441 ctcgctggat tacccggaga tcaccatcga agacgcttac gcggtgcagc gtgaatgggt
    16501 tcgcctgaaa atcgccgaag gtcgcacgct gaaaggccac aaaatcggcc tgacctcgaa
    16561 agcgatgcag gcaagctcgc agatcagcga accggattac ggcgcgctgc tggatgacat
    16621 gttcttccac gatggcagcg atatcccgac cgatcgcttt atcgtgccgc gcattgaagt
    16681 ggagctggct ttcgtgctgg caaaaccgct gcgtgggcca aactgcacgc tgttcgacgt
    16741 ctacaacgcc acggactacg tgatcccggc gctggagctg atcgacgctc gctgccacaa
    16801 catcgatccg gaaacccagc gcccgcgcaa agtgttcgac accatttctg ataacgccgc
    16861 caacgccggg gtgatcctcg gcggtcgtcc gatcaaacca gacgaactgg atctgcgttg
    16921 gatctccgcc ctgatgtatc gcaatggcgt gattgaagaa accggcgtcg ccgctggcgt
    16981 gctgaatcat ccggcaaacg gcgtggcctg gctggcgaac aaactcgcac cgtatgacgt
    17041 acaactggaa gccgggcaaa tcattctcgg tggttcgttc acccgcccgg ttccggcgcg
    17101 taagggcgac accttccacg tcgattacgg caacatgggt tccattagct gccgctttgt
    17161 ttaaggagag aacgatggaa aacagcttta aagcggcgct gaaagcaggc cgtccgcaga
    17221 tcggattatg gctggggctg agtagcagct acagcgcaga gttactggcc ggagcaggat
    17281 tcgactggtt gttgatcgac ggtgagcaca cgccgaataa cgtgcaaacc gtgctcaccc
    17341 agctacaggc gattgcgccc taccccagcc agccggtagt acgtccgtcg tggaacgatc
    17401 cggtgcaaat caaacaactg ctggacgtcg gcacacaaac cttgctggtg ccgatggtac
    17461 aaaacgctga cgaagcccgt gaagcggtac gcgccacccg ttatcccccc gccggtattc
    17521 gcggtgtggg cagtgcgctg gcccgcgcct cgcgctggaa tcgtattcct gattacctgc
    17581 aaaaagccaa cgatcaaatg tgcgtgctgg tgcagatcga aacgcgtgag gcaatgaaga
    17641 acttaccgca gattctggac gtggaaggcg tcgacggcgt gtttatcggc ccggcggatc
    17701 tgagcgccga tatgggttat gccggtaatc cgcagcaccc ggaagtacag gccgccattg
    17761 agcaggcgat cgtgcagatc cgcgaagcgg gcaaagcgcc ggggatcctg atcgccaatg
    17821 agcaactggc aaaacgctat ctggaactgg gcgcgctgtt tgtcgccgtc ggcgttgaca
    17881 ccaccctgct cgcccgcgcc gccgaagcgc tggcagcacg gtttggtgcg caggccaccg
    17941 ccgtgaagcc cggcgtgtat taatgccgga tgcgctgcgc ttatccggcc tacactcgca
    18001 ccaaaccgta ggccggataa ggcgtttacg ccgcatccgg caaaaagccg taccaaatcg
    18061 cggaccggat aaggcgttac gccgcatccg ccataaacct tgtcgtaccc tacaaaaatc
    18121 ccattagagg aagaaaaatg agcgacacct cacctgccat accggagagt atcgatccgg
    18181 cgaatcagca taaagcgctg actgccggac aacaggcggt tattaagaag ctatttcgcc
    18241 gcctgagcgt ctttctgttc gtgctgttta tcttctcgtt ccttgatcgc atcaacatcg
    18301 gctttgccgg actcacgatg ggacgcgacc tcggtctgag cgccaccatg tttggcctcg
    18361 ctaccaccct gttctatgcc gcttatgtca tcttcggcat tcccagcaac attatgctga
    18421 gtattgtcgg tgcgcggcgc tggatcgcca ccatcatggt gctctggggc atcgcctcta
    18481 ctgccaccat gtttgccact ggccccacca gcttgtacgt actgcgtata ctggttggca
    18541 ttaccgaagc cggctttctg cctggtattc tgctgtattt aaccttctgg tttccggcct
    18601 atttccgcgc ccgtgccaac gccttgttta tggtagcaat gccggtaacg acagcgttgg
    18661 gctcgatcgt ttccggctac attttgtcgc tggatggcgt catggcatta aaaggctggc
    18721 agtggctgtt tttgctggaa ggcttcccgt cggtattact cggcgtcatg gtgtggttct
    18781 ggcttgatga ctcaccggac aaagctaagt ggctgacgaa agaagacaaa aaatgcctgc
    18841 aagagatgat ggataacgat cgtctgacgc tggttcagcc agagggagcc atcagccacc
    18901 acgccatgca acaacgcagt atgtggcggg agatcttcac tccggtggtg atgatgtata
    18961 ccctggcgta tttctgcctg accaacacac ttagtgcgat cagcatctgg acaccgcaga
    19021 tcctgcaaag ttttaatcag ggcagcagta atatcaccat cggcatgctg gccgccgtac
    19081 cgcagatttg taccattctc gggatggtct actggagccg tcactcagat cgccgccagg
    19141 aacgaaggca tcacaccgcc cttccttatt tgttcgctgc cgcaggttgg ttactggctt
    19201 cggcaactga tcacaacatg atccagatgc tggggatcat tatggcttcg accggatcat
    19261 tcagcgcaat ggcgattttc tggacaacac cggatcagtc catcagcctg cgggcacgag
    19321 cgatcggtat tgcggtgatc aacgccactg gcaacattgg ttcagcgtta agtccgttta
    19381 tgatcggctg gttgaaagat ttgaccggca gctttaacag tggattgtgg tttgttgccg
    19441 cgctgctggt gattggtgcg gggattatct gggcgattcc aatgcagtcc tcccgtccgc
    19501 gagcgaccct gtaaggaacg acgatgtgtg accgtcagat tgccaatatt gatatcagca
    19561 aagagtacga tgaaagcctg ggcacggacg atgtgcatta tcagtcgttc gcccgcatgg
    19621 cggcattttt tggccgccat atgctgccac atcgccacga acagtacttt cagatgcatt
    19681 tcctcaatag cggacagatt gagctacagc ttgacgatca tcgctactcg gtggaagcgc
    19741 ccctgtttgt cctgacgccg ccgtcag
//
LOCUS       DAZPDU010000073        19764 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZPDU010000073 DAZPDU010000000
VERSION     DAZPDU010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19764
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.074"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..369
                     /locus_tag="ACHURS_002322"
     CDS             <1..369
                     /locus_tag="ACHURS_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFR1238037.1"
                     /translation="AMMYAETRNMTPYGNVGIANKTQNFEAVAQYQFDFGLRPSLAYV
                     YSKGKDLGGNDYNNNGHQEYVDQDLVNYVEIGATYYFNKNFSTYVDYKINLLDKDDDF
                     YDNNGIATDDVVGVGLVYQF"
     gene            complement(403..813)
                     /locus_tag="ACHURS_002323"
     CDS             complement(403..813)
                     /locus_tag="ACHURS_002323"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1238038.1"
                     /translation="MLNIIHAKFSLLSNVVASGLNDFRPLSKVRGGGERFSAVKYCGT
                     DEYLYRGLVEKRLFHALFLQIFLYGKRVCYGLIHKEMHLGVGLKSRWLMLLCQEGQGK
                     VLYRYALAIACAADGDEMPIKKPEFDEGRTQASV"
     gene            960..1811
                     /gene="hchA"
                     /locus_tag="ACHURS_002324"
     CDS             960..1811
                     /gene="hchA"
                     /locus_tag="ACHURS_002324"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFR1238039.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAD"
     gene            complement(1919..3277)
                     /gene="hprS"
                     /locus_tag="ACHURS_002325"
     CDS             complement(1919..3277)
                     /gene="hprS"
                     /locus_tag="ACHURS_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFR1238040.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDVASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRIMLPQ
                     RN"
     gene            complement(3277..3948)
                     /gene="hprR"
                     /locus_tag="ACHURS_002326"
     CDS             complement(3277..3948)
                     /gene="hprR"
                     /locus_tag="ACHURS_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFR1238041.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRNNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            4081..4494
                     /gene="hiuH"
                     /locus_tag="ACHURS_002327"
     CDS             4081..4494
                     /gene="hiuH"
                     /locus_tag="ACHURS_002327"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFR1238042.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            4603..5607
                     /gene="msrP"
                     /locus_tag="ACHURS_002328"
     CDS             4603..5607
                     /gene="msrP"
                     /locus_tag="ACHURS_002328"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFR1238043.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPTAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            5608..6243
                     /gene="msrQ"
                     /locus_tag="ACHURS_002329"
     CDS             5608..6243
                     /gene="msrQ"
                     /locus_tag="ACHURS_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFR1238044.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFKRLRKQVHNKLSV"
     gene            6500..7150
                     /gene="zinT"
                     /locus_tag="ACHURS_002330"
     CDS             6500..7150
                     /gene="zinT"
                     /locus_tag="ACHURS_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFR1238045.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKIKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQFLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            7493..8023
                     /locus_tag="ACHURS_002331"
                     /pseudo
     CDS             7493..8023
                     /locus_tag="ACHURS_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            complement(8593..8682)
                     /locus_tag="ACHURS_002332"
     tRNA            complement(8593..8682)
                     /locus_tag="ACHURS_002332"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8646..8648),aa:Ser,seq:cga)
     gene            8776..9573
                     /gene="mtfA"
                     /locus_tag="ACHURS_002333"
     CDS             8776..9573
                     /gene="mtfA"
                     /locus_tag="ACHURS_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFR1238046.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTGLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            9674..9749
                     /locus_tag="ACHURS_002334"
     tRNA            9674..9749
                     /locus_tag="ACHURS_002334"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9707..9709,aa:Asn,seq:gtt)
     gene            10063..17128
                     /gene="yeeJ"
                     /locus_tag="ACHURS_002335"
                     /pseudo
     CDS             10063..17128
                     /gene="yeeJ"
                     /locus_tag="ACHURS_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin YeeJ"
     gene            complement(17390..18442)
                     /locus_tag="ACHURS_002336"
     CDS             complement(17390..18442)
                     /locus_tag="ACHURS_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFR1238047.1"
                     /translation="MFLASLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEGMRKKNLKTMLFISQLKAKTNFQVVGITMNCYSPLCKNASRMDQ
                     KLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIAFNLEGSSQ
                     ERTFSLSIAENLIAKIQSETDIPIVIVHGPKGEDKARALVDCYNNVYRLSLPPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            18757..>19764
                     /gene="shiA"
                     /locus_tag="ACHURS_002337"
     CDS             18757..>19764
                     /gene="shiA"
                     /locus_tag="ACHURS_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFR1238048.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRVIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHYQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MA"
BASE COUNT         5513 a         4657 c         4411 g         5183 t
ORIGIN      
        1 gcaatgatgt atgctgaaac ccgcaatatg acaccatatg gtaatgtggg tatcgcaaat
       61 aaaacccaga actttgaagc tgtagcacag tatcagttcg attttggtct gcgcccatct
      121 cttgcatatg tttattcaaa aggcaaagac ctcggcggta acgactataa caacaatggt
      181 catcaagagt atgttgatca ggatctggtc aactatgttg aaattggagc gacctactat
      241 ttcaataaga acttctcaac atatgttgac tataaaataa acttgttgga taaagatgac
      301 gatttctatg acaacaatgg catcgcaaca gatgacgttg ttggtgtggg cttagtctac
      361 cagttctaat cagccaacgt gctgcggcgt gttactgtaa aactaaacac tagcctgagt
      421 tcgtccctca tcgaattcag gcttttttat tggcatttca tctccatctg cggcacacgc
      481 aattgccaat gcatatcgat acagcacctt accctgcccc tcctggcaca ataacatcaa
      541 ccagcgactc ttcaaaccaa cacccaaatg catttcttta tgaattaatc cgtaacaaac
      601 acgcttccca tatagaaata tttgcaaaaa tagtgcatga aataatcttt tctctaccaa
      661 tccgcgatat aaatattcat cagtaccaca atatttcaca gcactaaatc tctccccgcc
      721 gccccgtacc tttgataatg gtctaaaatc attgaggcca cttgcgacga cgttgctcaa
      781 taaggaaaat ttggcgtgaa taatattcaa caccagtgca gcatcatcat tacatactta
      841 cacccgccga ctaccgcttt cgatatattg actcaatttt ccagactggt taaaaatagc
      901 gaccagctca gtcgcaaata tagtgactac cctaactaag caacaataag gaatacacta
      961 tgactgttca aacaagtaaa aatccgcagg tcgatattgc tgaagataat gcattcttcc
     1021 cttcagaata ttcgcttagc caatatacca gtcccgtctc tgatcttgat ggcgtggact
     1081 atccaaaacc gtatcgcggt aagcataaaa ttctggtgat cgccgcggac gaacgttatt
     1141 tgccaaccga taacggaaaa ctgttctcga ccggtaacca tccgattgaa acgttgctgc
     1201 cgttgtatca tctccatgct gctggtttcg aattcgaagt ggcgaccatt tccggtctga
     1261 tgaccaagtt tgaatactgg gctatgccgc acaaagatga aaaagtaatg ccattctttg
     1321 agcagcataa atcgttgttc cgcaatccga agaaactcgc ggatgttgtc gccagcctca
     1381 acgctgatag cgaatatgca gcaatctttg ttcctggtgg tcatggcgca cttattggtt
     1441 taccagaaag ccaggacgta gctgctgctt tgcagtgggc aatcaaaaat gaccgttttg
     1501 ttatctccct ttgccacggc ccggcggctt ttctggcgct tcgccacggc gataacccac
     1561 tgaatggtta ttccatttgc gcattcccag acgccgcaga caaacaaacg ccagagatag
     1621 gctatatgcc gggtcatctc acctggtact tcggcgaaga gctgaagaaa atgggcatga
     1681 acattattaa tgacgacatc accgggcgag tacataagga ccgtaaagtt ctcaccggcg
     1741 acagtccttt cgcagcgaat gcgttgggta aactggcggc gcaggaaatg ctggcagctt
     1801 atgcggatta atccctgtta cgtttgaatg taaacgcata atcaatcgca gccacgttaa
     1861 tactcacgac gtggctgcgt tttttcatgg ttgatttatg gcgtactgaa gccctatgtt
     1921 aatttctttg cggtaacata atccggaaca cattatgctt attgagatag tgatacgtag
     1981 cacttccccc atgtaattcg gcaatcgctt tgactaaaga aagaccaagt ccctgaccta
     2041 cggaatgacg cgaattatct ccccgccaaa atctacggaa gagtttttca ggctcatgaa
     2101 ttttcgttcc aggactggcg acatcaatat taagatagcc gttggtatca agaaaactgg
     2161 ttatatgaat acgcgatttt tctggtgaat atctaatggc attaacaata agattcgata
     2221 acattcgttg cagtaaaatt ttatccgcaa agatttgctg attgcactcg accttaaagc
     2281 aaatctcttt ctcgtctgaa aggtattcaa gataatccaa caaattttcg acttccttat
     2341 tgagagaaag cgagtccagt ttcaccaaaa cattgttttt atctgcccgg gcaagaaaca
     2401 gtatgttctc tgttaaccgc gaaatatttt ccagctcttc aatgttaccg gcaattgttt
     2461 tttgatattc agcgatactt ctggtttgac tgagcgtaac ctgattctga cccagtaatg
     2521 cattaatggg cgttctaagt tcatgagcga gatcgtcagc aaattgactt agacgctcaa
     2581 aatctttgac taaagcatga tgcattttat tcaacgcctg cccaagaggt tttagttctc
     2641 tcggtaatgc gctaacctca acaggctccc ggctatcgtt ataattcagc gcttccgtta
     2701 caccactcaa ctttttgatc tctcgtaatc ccgttctgat taacagcgga cttaatactg
     2761 agcaaagtac aatggcgaca atgcaaatta taatgctatt aattttatac tgttcaagca
     2821 tgttatgtct ggctgaagcc aatttagcca cagtaaccgt taatgatggc gaaacttcat
     2881 caatattaat tcgtaaagca tcaatctcag tatcattaat aatgcttctg taaatgccag
     2941 ctgcgctgat tgtctcacta gcaggtatgt tatttaacat gtcatcactg acatttgtcc
     3001 ggttaacaat tttattgatg ccatcaccat gaatgatcaa gatatcctga ctaacatcca
     3061 tcatccggtt aaagtataca ggtaacgtat ctggatttac cccatcaatt aacaactgct
     3121 tgatctgtgc tgtccggtta atgagtgttg tatcatcgcg ccatttcaac tcacttgcca
     3181 ggccattata gagagtccag acaattccag cgccagcaac agacagcagc aatataaaaa
     3241 gcaaggttaa acggacggtt atagataatc ttttcattat ttttttaccg ctacgaatga
     3301 atagcccatc ccccggattg tggcgattag cttttcagga aaaggatcat caacttttgc
     3361 gcggagcctg cgaatggcga catccaccgt attggtatca ctatcaaagt tgattcccca
     3421 aatttcactc gcaataaccg ttctgggtat aatttcgcca gctctggagg ccagtagcca
     3481 aagtaactga aactccttgc gcgtcagtgt aatactgata ttgttcctgc tcacactttg
     3541 acttacagag tccattctta agccactgat ttctaatgtt gaattcaaag cgtgatgttg
     3601 ccttaattgt gcccgaaccc ttgccagcaa ctcagaaaat gaaaaaggtt ttaccagata
     3661 atcatttgct ccactgtcca gccctctgac tctgtcatcg acagaatccc ttgcagtaag
     3721 gcaaataaca ggggtttgct ttgctgttct taacgtttgt aagatctgcc agccatccat
     3781 acccggaagc ataatatcca gaatgatcaa tgcataatca tccttcagcg caagataaag
     3841 cccatctctg ccatcagaaa cggcatcaat gacataaccc gcttcggaaa gcccctgcgt
     3901 tacccactcc tgggtccttt gattatcttc aataagtaaa atcttcatga aaataatatg
     3961 ccataaatca tatgttatag aaacagccta gttcattaca aaattgtaat gctgctgtaa
     4021 ggttaccctg gccgcttttt cgctatcctc aaaactcatt cacatgacaa ggatataaac
     4081 atgttaaagc gttatttagt actctccgta gtaacggcag cattttcatt accttctttg
     4141 gtttatgccg cacaacaaaa cattcttagc gtgcacattt tgaaccagca aaccggaaaa
     4201 cccgctgccg acgtgacagt cactcttgaa aagaaagcgg acaacggctg gttacaactt
     4261 aataccgcca aaacagataa ggatggacga attaaggcac tgtggcccga gcaaactgca
     4321 actacgggcg attaccgtgt cgtatttaaa accggggact atttcaagaa acaaaatctt
     4381 gaaagtttct tccctgagat ccccgttgaa tttcatatta ataaagtgaa cgagcattat
     4441 catgtgcctt tattacttag ccaatatggg tattcaacct atcgtggcag ttaatgcata
     4501 aatatgtaaa agcagatctc tgctactgtt agggaggaca ctccctgaca gattaacagt
     4561 aaacggctct tgctggctaa cgacaaaaaa gtgtgatggc ttatgaaaaa gaatcaattt
     4621 ttaaaagaat cagatgttac ggccgagtcg gtattcttta tgaagcgtcg acaggtgtta
     4681 aaagcactgg gcatcagcgc agctgcactt tctttgcctc acgctgcgca tgccgatctg
     4741 cttagctggt ttaaagggaa cgatcgcccg cccgcccccg ccggaaaacc gctggagttc
     4801 agcaagccta ccgcctggca aaataatctg ccactgacgc cagtagataa agtctccggt
     4861 tataacaact tctatgaatt cgggctggat aaagccgatc ccgccgctaa tgctggtagc
     4921 ctgaaaaccg atccatggac actgaaaatc agcggcgaag tggcaaaacc attgaccctc
     4981 gatcacgatg atttaacccg tcgcttcccg ctggaagagc gtatttatcg tatgcgctgc
     5041 gtggaggcat ggtcgatggt ggtgccgtgg attggttttc cgctgcacaa attgctggcg
     5101 cttgccgaac ccaccagcaa tgcgaagtat gtcgctttcg aaacaattta tgcaccggaa
     5161 caaatgccag gccagcagga ccgctttatc ggcggcgggc tgaaatatcc ttatgtcgaa
     5221 ggattgcgtc tcgacgaagc aatgcatccg ctcacactga tgaccgtggg tgtttatggc
     5281 aaggcgttac cgccacaaaa tggcgcgccg gtacgactga ttgtgccgtg gaaatatggc
     5341 tttaaaggga ttaaatcgat agtcagtatt aagctgaccc gcgagcgtcc gccaaccacc
     5401 tggaatctgg cagcgcctga cgaatacggt ttttacgcca acgttaatcc gcatgttgat
     5461 cacccgcgct ggtcacaggc taccgaacga tttattggtt caggcggcat cctcgatgtt
     5521 cagcgccagc caacgctact gtttaatggt tacgccgacc aggtggcatc gctgtatcgt
     5581 ggcctggatt tgcgggagaa tttctgaatg cgtctgacag caaaacaggt gacatggctg
     5641 aaagttagcc tgcatcttgc cggattgttg ccgtttctct ggctggtctg ggcgatcaat
     5701 cacggtggac tgggtgccga tccggtgaaa gatattcagc attttaccgg tcgcactgca
     5761 ctgaaatttt tgctggcgac cttgttaatc acacctctgg cacgctacgc aaaacagccg
     5821 ttattgatac gcactcgccg cctgttaggc ttatggtgct ttgcctgggc gacactgcat
     5881 ttaaccagtt acgcattgct ggagctgggc gtgaataatc tggcgttatt aggcaaggag
     5941 ttaattaccc ggccttattt aacgttagga attatcagct gggttatttt gcttgcttta
     6001 gcgtttactt caacccaggc gatgcagcga aaactcggca agcactggca acagttgcat
     6061 aatttcgtct atcttgtcgc gatcctggcc ccaatacatt atctgtggtc agtgaagatt
     6121 atttcaccgc agccgctcat ctacgctggg ctggctgtac tgcttttagc cttacggtat
     6181 aagaagttgc gttccctttt taagcggtta cgcaagcagg ttcacaacaa attatctgta
     6241 taacttcata ttgccgacaa agtacgcgtt atccctgccg gatgtggcgc gagcgccctg
     6301 tccggcctgc gcagatcaca tcaggttatg tttcgtttgt catcaacctc aaattatcaa
     6361 cataattcat tttatttcaa taagatagat aagtacacct gagaaaacta tgctctcatc
     6421 tcctaagagt tgttgcattt tgccatatgt tacaatataa cattacacat catatacaat
     6481 gactctggag gaaactgttt tggcaattcg tcttcacaaa ctggctgttg ctttaggtgt
     6541 ctttattgtt agcgctcctg ccttttcgca tggtcatcac tcacacggca aacccttaac
     6601 agaggtcgaa caaaaagctg ctaatggtgt ttttgatgat gccaatgtac aaaaccgaac
     6661 gctcagtgac tgggatggag tctggcaatc cgtttatcct ttactgcaaa gtggcaaact
     6721 tgaccccgtc tttcagaaga aagcggatgc agataaaatt aaaacatttg ctgaaattaa
     6781 agattattat cacaaaggtt atgcaacaga tatcgagatg attggcattg aggacggcat
     6841 tgttgaattc catagaaata atgaaacaac atcctgtaaa tatgattacg atggatacaa
     6901 aatactcacc tataaatcag gcaagaaagg cgttcgttac ttattcgaat gtaaagatcc
     6961 tgaaagcaaa gcccctaaat atatacaatt tagcgatcat ataattgcac cacgaaaatc
     7021 atctcatttt cacattttta tgggtaatga ttcacagcaa tttttattaa atgaaatgga
     7081 aaactggcca acgtattatc catatcagtt gagtagcgaa gaagtggtcg aggaaatgat
     7141 gtctcattga gtattctcat gataacgcct cgatgccgct ttagtaagtc atcataactg
     7201 ccactggtcc atccacaaac gccactgaac gcaagctagc tacagacacg ctcatcacta
     7261 tgacgtgtct gtatattaat aagctaaccc gcattgagtt aaccaataac ggattccata
     7321 cacaatacgg ccaacagaga aagatacctg tgctcacgcc attgcttata ttggctggtt
     7381 acaatgtgca ctatcaattt tttaaataac aaattcttaa aaccacatct gtataaggaa
     7441 tttttaaggt tcgtgggata gcttgactgt aaaaattaca ggagctacaa aaatgaaccg
     7501 attctcaaaa actcaaattt atttacattg gataacgctg cttttcgttg caataaccta
     7561 tgccgcgatg gaactccgtg gctggtttcc taaaggtagt agtacttatc tgctgatgcg
     7621 agaaacacat tacaatgcgg gtatattcgt ttgggtgtta atgttttcac gcctgattat
     7681 aaaacaccgt tatagtgatc cttctattgt accaccgcca cctgcctggc aaatgaaagc
     7741 ggcttcgcta atgcacatca tgctttatat aaccttcctt gcacttcctc tgctggggat
     7801 tgctttgatg gcttacagtg gaaaatcgtg gagtttcctt ggtttcaatg tgtctccctt
     7861 tgttacccca aacagcgaaa ttaaagcact gataaaaaat attcacgaaa cctaggcaaa
     7921 tataggttac tttttaatcg cagctcacgc tggcgcagca ctctttcatc actacattca
     7981 gaaagataat actctgttac gaatgatgca tcgccgcaaa taagtcgatc ccctgacaag
     8041 gaaatgtgaa ccatgtacat caacgcgtca taaatgttta cgcaagtgca caatgcatcc
     8101 tttctactat caacaaggag gagcagaaac tgtgtaaatc gtacaatggt aagacttatc
     8161 aaaaatacag gttaattttg gtatttaaac cttttttact tgttagtatg atacaaaggc
     8221 tttcaaaaaa gctgggaaat tcaacaactc ttggaaatta tggactttgt cccgcggctt
     8281 taatctgacg gccgcgttcc ttttttgact caattattga ccccttctct acgcaacttc
     8341 agttcccacc accaactttg cggcagtttc ataggatgaa tgtctaaaag aataatggtg
     8401 agcgataaga aaacgactga ataactgcac attttcgctc gaaagcttcc tgtcagatcc
     8461 atagcgaatc aagtgctgaa tgtcacagta tcgaacagaa aacagtgacg atctaaccct
     8521 tcaagaatat tctacaattg ttctgtttag gaaaagcaag gcgggaagtc aggagataag
     8581 tcattgataa agtggcggag agagggggat ttgaaccccc ggtagagttg cccctactcc
     8641 ggttttcgag accggtccgt tcagccgctc cggcatctct ccgttcagat ggttgccatg
     8701 atgccaggaa atttggcatt ttaacagtcc ctgtccgtgc aattttgttc aagtgacgag
     8761 tttgcgagca aaacgatgat taagtggccc tggaaagtac aagaatcagc acatcaaact
     8821 gcccttccct ggcaggaagc actatcgatc ccccttttaa cgggtctgac ggaacaggaa
     8881 caaagcaaat tagtcactct tgccgaacgt tttttacagc aaaagcggct tgttccttta
     8941 cagggctttg aactggattc attaagaagc tgccggatag cacttctatt ttgcctaccc
     9001 gttctggagt taggactgga atggctggat ggttttcatg aagtcttaat ttatcctgcg
     9061 ccatttgtgg tcgatgatga atgggaagac gatatcggtc tggtgcataa ccaacgtatt
     9121 gttcagtcag gtcagagctg gcagcaaggg cctatcgttt tgaactggtt ggatatacaa
     9181 gattcttttg atgcatctgg ttttaacctg attattcatg aagtcgctca taagctggac
     9241 acccgtaacg gcgatcgcgc cagcggagtt ccctttattc cgttgcgtga ggttgctggc
     9301 tgggaacacg atcttcatgc tgcaatgaac aacattcagg aagaaatcga attggttggc
     9361 gagaatgcgg cgagcattga tgcttatgca gccagcgatc ctgctgaatg ttttgccgta
     9421 ctttctgaat atttctttag cgccccagaa ctttttgctc ctcgtttccc ttcattgtgg
     9481 caacgtttct gccaatttta tcaacaagat cctttgcaga gactgcatca cgctaatgat
     9541 acagactcgt tttcggcgac gaatgttcat taattaacaa ctttgcagat taattaacca
     9601 attgaaatga cttatgaaat ttagtgttga cagacaaggt accgctaagt aatatgtgcc
     9661 ccgttcacac gattcctctg tagttcagtc ggtagaacgg cggactgtta atccgtatgt
     9721 cactggttcg agtccagtca gaggagccaa attctaaaaa ttcgcttttt tagcgcaatg
     9781 tcactgacct tagttgaaca ttgtttttta acggatagcg ggtttttaac accttaagcg
     9841 ccctcgacct ttatggttga gggcgttttg ctatgaacgc catcaccatt ttcccctcga
     9901 ttataaaact tgagttattc agtagtctcc cctcttgcaa ctcacaccca aaactgccta
     9961 acgaaaagtt attaattttc aatcgtattg ctatcagtat ttacattttt tcgctgtgct
    10021 agaaagggcg catttatgtt agctcgttca gggaaggtaa gcatggctac gaagaagaga
    10081 agtggagaag aaataaatga ccgacaaata ttatgcggga tgggaattaa actacgccgc
    10141 ttaactgcgg gtatctgtct gataactcaa cttgcgttcc ctatggctgc ggcagcacaa
    10201 ggtgtggtaa acgccgcaac ccaacaacca gttcctgcac aaattgccat tgcaaatgcc
    10261 aatacggtgc cctacaccct tggagcgctg gaatcggccc aaagcgttgc cgaacgtttc
    10321 ggtatttcgg tggctgagtt acgcaaactc aaccagtttc gtacgtttgc tcgaggtttt
    10381 gataatgtcc gccagggtga tgaactggat gtcccggcac aagttagtga aaaaaaatta
    10441 accccgccgc cgggtaatag cagtgacaac ctcgagcaac agatagccag tacttcacag
    10501 caaatcgggt ctctgctcgc cgaagatatg aacagcgagc aagcggcaaa tatggcgcgt
    10561 ggatgggcct cttctcaggc ttcaggcgca atgacagact ggttaagccg cttcggtacc
    10621 gcaagaatca cgctgggcgt ggatgaagat tttagcctga agaactccca gttcgatttt
    10681 ctccatccgt ggtatgaaac gcctgataat ctctttttca gtcagcatac tctccatcgt
    10741 actgacgagc gtacgcagat taacaacggc ttaggttggc gtcatttcac tcccacatgg
    10801 atgtcgggca tcaacttctt tttcgaccac gatcttagcc gttaccactc ccgcgccggc
    10861 attggcgcgg agtactggcg cgactatcta aaattaagca gtaacggcta tttgcgactg
    10921 accaactggc gcagcgcacc tgaactggac aacgattata aagcacgccc ggccaatggc
    10981 tgggatgtac gcgcagaaag ctggctaccc gcctggccgc accttggcgg taaactggtc
    11041 tatgaacagt attatggcga tgaagtggcc ctgttcgata aagacgatcg gcaaagtaat
    11101 cctcatgcca taaccgctgg acttaactat acccccttcc cgctgatgac cttcagcgcg
    11161 gagcaacgcc agggtaaaca gggcgaaaat gacacccgtt ttgccgtcga ttttacctgg
    11221 caacctggca gcgcaatgca gaaacagctt gacccgaatg aagtcgctgc acggcgtagc
    11281 cttgcaggca gccgttatga tctggtggat cgcaacaaca atatcgttct ggaatatcgc
    11341 aaaaaagaac tggttcgcct aaccctgaca gaccccgtga cagggaagtc aggagaagtg
    11401 aaatcactgg tttcgtcgct acaaaccaaa tatgccctga aaggctataa cgtcgaagcc
    11461 accgcactgg aagctgccgg tggcaaagtg gtcacaacgg gtaaagatat tctggttacc
    11521 ctgccggctt accggttcac cagtacgcca gaaaccgata acacctggcc gattgaagtc
    11581 accgccgaag atgtcaaagg caatttgtcg aatcgtgaac agagcatggt ggtcgttcag
    11641 gcacctacgc taagccagaa agattcctcg gtatcgttaa gtacccaaac attgaacgcg
    11701 gattcccatt caaccgccac actgactttt attgcgcatg atgcagcagg taatcctgtt
    11761 gtcgggctgg tgctctcgac gcgtcacgaa ggtgttcagg acatcaccct ttctgactgg
    11821 aaagataatg gtgacggaag ctatacccag atcctgacca caggagcgat gtctggcacg
    11881 ctgacgctga tgccacagct gaatggtgtg gatgcggcta aagcccccgc cgtggtgaat
    11941 atcatttctg tttcgtcatc ccgaactcac tcgtcaatta agattgataa ggaccgttat
    12001 ctctccggca atcctatcga ggtgacggta gaactgagag atgaaaatga caaacctgtt
    12061 aaggaacaaa aacagcaact gaataacgca gtcagcatcg acaacgtgaa accaggagtc
    12121 actacagact ggaaagaaac cgcagatggc gtctataagg cgacctatac cgcctatacc
    12181 aaaggcagtg gacttactgc gaagctattg gaatgaagat ttgcataccg ctggatttat
    12241 catcgacgcc aacccgcagt cagcgaaaat tgcgacatta tctgccagca ataatggtgt
    12301 actcgccaat gagaatgcag caaacaccgt ctcggtcaat gtcgctgatg aaggaagcaa
    12361 cccaatcaat gatcataccg tcacgtttgc ggtattaagc ggatcggcaa cttccttcaa
    12421 caatcaaaac accgcaaaaa cggatgttaa tggtctggcg acttttgatc tgaaaagtag
    12481 taagcaggaa gacaacacgg ttgaagtcac ccttgaaaat ggcgtgaaac aaacgttaat
    12541 cgtcagtttt gtcggcgact cgagtaccgc gcaggttgat ctgcagaagt cgaaaaatga
    12601 agtggttgct gacggcaatg acagcgccac aatgaccgcg accgtccggg atgcaaaagg
    12661 caacctgctc aatgacgtca tggtcacttt caatgttaat tcagcagagg cgaaactgag
    12721 ccaaaccgaa gtgaatagcc acgacgggat cgccacagct acgctgacca gtttgaaaaa
    12781 tggtgattat agggttacgg cctctgtgag ctctggttcc caggctaatc aacaggtgaa
    12841 ttttatcggt gatcaaagta ctgctgccct gaccctcagt gtgccttcag gtgatatcac
    12901 cgtcaccaac acagctccgc aacatatgac tgcaaccttg caggataaaa atggcaaccc
    12961 actaaaagat aaagaaatca ccttctctgt gccaaacgac gtcgcaagta agttctcgat
    13021 tagcaacgga ggaaaaggca tgacggatag taacggggtt gcaatcgcct ccctgaccgg
    13081 cacgttagcg ggcacgcata tgatcatggc tcgtcgggct aacagcaatg tcagcgatgc
    13141 acagccaatg acgtttgtgg cggataaaga cagagcggtt gtcgttttgc aaacatcgaa
    13201 agcggaaatc attgggaatg gcgtggatga gacaactctg acagcaacag tgaaagatcc
    13261 gtcgaatcat ccggtggcgg ggataacggt aaacttcacc atgccacagg acgttgcggc
    13321 aaactttacc cttgaaaata acggtattgc catcactcag gccaatgggg aagcgcatgt
    13381 cacgctgaaa ggtaaaaaag cgggcacgca tacggttacc gcaacgctgg gtaataacaa
    13441 taccagtgat tcgcagccgg taacatttgt ggcggacaaa gcctcggctc aggttgtcct
    13501 gcagatatca aaagatgaga tcacaggtaa tggcgtcgat agcgcaacgc taactgcaac
    13561 ggttaaagat cagttcgaca atgaggtgaa taatcttccg gtaacattca gctcagcctc
    13621 ttcaggactc accctgaccc cgggagtaag taataccaat gagtctggca tcgcgcaggc
    13681 cactctcgca ggcgttgcct ttggtgagca gacggtcact gcatcactgg ctaataatgg
    13741 tgccagcgac aacaaaactg tgcattttat tggcgacaca gcggcggcaa aaattatcga
    13801 gttgacgcct gtcccagaca gcataatcgc cggtaccccg cagaacagct ccggcagcgc
    13861 catcaccgcc acagtcgttg ataataatgg ctttccggtg aaaggtgtga ctgtgaactt
    13921 caccagcaga acaaactctg ccgaaatgac gaatggcggc caagccgtaa cgaacgaaca
    13981 gggtaaggct accgtcactt ataccaatac ccgctcctcg atagaatcag gagcgagacc
    14041 ggataccgtt gaggccagtc tggaaaatgg tagctccacg cttagcacat caattaatgt
    14101 caacgctgat gcgtctacgg cacatctcac ctggctacag gcactttttg atacagtctc
    14161 cgcaggcgag acaaccagtc tgtatattga ggtgaaggat aattacggca acggtgtccc
    14221 ccagcatcag gtgaccctca gcgtttcacc aagtgaaggc gtgaccccca gtaataacgc
    14281 tatatatact accaaccacg acggcaattt ttacgcaagc tttaccgcta caaaagccgg
    14341 ggtttatcaa gtgacggcaa ccctcgaaaa tggcgattcg atgcaacaaa cagtgaccta
    14401 tgtgccgaac gtcacgaatg ccgaaatcac gctggcaacc tcgaaggatc cggtgattgc
    14461 cgacaataac gatctcacga cactaacagc aacagtcgct gatacagagg gcaatgcgat
    14521 agccaacact gaagtaacat ttactctgcc ggaagatgtt aaggcgaact tcacgctgag
    14581 cgatggcggt aaagcgatta ctgatgctga aggcaaagcg aaagtcacgc tgaaaggtac
    14641 aaaagcaggc gctcatactg ttacagcatc gataactggc ggtaagagtg agcagttggt
    14701 ggtgaacttt actgcggata cgctcactgc gcaggttaat cttaacgtta ccgaggacaa
    14761 ttttatcgct aataacgtcg ggatgaccag gctgcaggca acagtgactg atggaaacgg
    14821 caacccgtta gccaatgagg cggtgacatt cacgctaccg gcagatgtga gcgcaagctt
    14881 tactctcgga caaggcggtt ccgccattac tgacatcaac ggcaaggctg aagttacact
    14941 gagcggtaca aaatccggca cctaccccgt gacagttagc gtgaacaatt atggtgtcag
    15001 tgatacgaaa caggtgactt tgattgccga tgctggtacc gcaacactag cctccttaac
    15061 ctctgtatac tcattcgtcg tcagcacgac cgagggcgcg accatgactg caagcgtcac
    15121 tgacgctaac ggcaacccgg tagaaggcat aaaagttaat ttccgcggaa cctccgtcac
    15181 gctaagcagc accagcgttg aaacggatga tcggggtttc actgaaattc ttgtgacaag
    15241 caccgaagtc ggactgaaaa cagtttcagc ctctctggca gataaaccta ctgaagtcat
    15301 atcgcgatta ctgaatgcaa aagcagatat taattctgca acgattacca gtctggagat
    15361 acctgaaggt caggtcatgg tcgcacaaga cgtagcagtt aaagctcacg tcaacgacca
    15421 gtttggcaac ccgattctta atgaatctgt aacattcagt gcagagccac cagagcacat
    15481 gaccatcagc caaaatattg tctctactga tacgcatggt atagccgagg tctccatgac
    15541 gcccgaaaga aacggttcgt atatggtgaa agcatccctg gcgaatggag cctcacttga
    15601 gaaacaactg gaggctattg atgaaaaact gacactctcg gcgtccagcc cgcttatcgg
    15661 tgtcaattcc ccaacaggtg caactctgac ggcaacgcta acttctgcaa atggcactcc
    15721 agtggagggt caggtcatca actttagcgt aacgccagaa ggtgcgacgt taagtggcgg
    15781 aaaagtgaga actaactctt ctggtcaggc tccagtcgtt ctgaccagca ataaagtcgg
    15841 tacatatacg gtgactgcat cgttccataa cggcgtaaca atacagacac agacaaccgt
    15901 gaaagtcact ggcaactcaa gcaccgccca tgttactagc tttatcgctg atccatcgac
    15961 tatcgccgcc accaacagtg atttaagtac cttaaaggca acggttgagg atggcagtgg
    16021 taacctgatc gaaggtctca ctgtgtactt tgccttaaaa agcggctctg ccacattaac
    16081 gtcattaaca gcggtgaccg atcaaaacgg aatcgcgaca acaagcgtga aaggagcgat
    16141 gacaggtagc gtcacggtaa gcgcagtcac gaccgctggt ggaatgcaaa cagtagatat
    16201 aacgctggtg gcaggcccag cagacgcctc aaagtcagtc cttaagaaca atcggtcatc
    16261 attgaaagga gactttaccg atagtgctga gctacatctt gttctgcacg atatatcagg
    16321 caatccgatc aaagtttctg aagggctgga atttgtacag tcaggtacca acgtgcccta
    16381 tgtgcaagtt agtgcaattg actacagtaa aaatttctca ggcgagtaca aagccactgt
    16441 tacaggaggc ggagagggta tcacaacgct gatccctgta ttgaatggtg ttcatcaagc
    16501 tggtctgagt accacaatac aattcactcg cgcagaagac aaaataatga gcggtacagt
    16561 atcagtcaat ggtactgacc taccgacaac tacattccct tcgcaggggt tcaccggggc
    16621 gtattatcag ttgaataatg acaactttgc cccaggaaaa acggcggctg attatgagtt
    16681 ttcaagctct gcctcctggg tcgatgttga tgctaccggt aaagtgacat ttaaaaatgt
    16741 cggcagcaat tcggaaagga ttacggcgac accaaaatca ggaggcccta gctatgtata
    16801 cgaaatccgt gtgaagagtt ggtgggtgaa cgccggcgag gctttcatga tatacagcct
    16861 tgctgaaaat ttttgcagca gcaatggcta cacgctcccc agagcaaact atttaaacca
    16921 cagtagttcc cgaggcatcg ggtcactgta cagtgaatgg ggagatatgg ggcattacac
    16981 gactgaagct ggttttcaat caaatatgta ttggtcatct agtcccgcaa actcaagcga
    17041 acaatacgta gtttccctgg caacaggtga tcaaagcgta tttgaaaagc ttgggtttgc
    17101 ttatgcgaca tgttataaaa acctctgatt ttccgttata atttcttaaa gagtgctcca
    17161 ttcggagctc tcttttttac attccctcta tagagcttgc ccttctctca cagaaaatga
    17221 ataaataaaa atgcggcacc gccagaattg cgttcgatgt gactatgatc gtatccggtg
    17281 agtactgctt tgattcaaag gctggttttc tgtgatgatt gaccagcctc agagtattga
    17341 tgacaccacc gttcataaca gatcggcatt actatgcata agtactgtct catattctcg
    17401 ccaaaacact ttttaatgcc tttgcgaatt catccagact tatattgtca attttttgcc
    17461 caacgacgac tgactccgag acatcggcca ttgcgggcca tcgcgttttg taatcagcat
    17521 aaattgcaac aacaggggta ttataggcac ttgccatatg taatattgag gtgtcaggag
    17581 ttattgcgat ataagcatct tttatgattg ctgctgaacg tttaatcgag ggtggtaagg
    17641 ataaacggta gacattatta taacaatcca ctaatgccct ggctttatct tcacctttgg
    17701 gtccatgaac gatcactatt ggtatatctg tttcactttg aatttttgct attagatttt
    17761 ctgcaatcga taatgaaaat gtacgttcct gcgagctacc ttctaaattg aacgcaatgt
    17821 aagatcctaa cgatcgcatt tcctcgcgca cctcatcgag tacatcctca cttagtggca
    17881 attcatatat tggcctgact gctggaaaac ccgcctcacg catcataaat gcccatgtca
    17941 taggtacggg agcccggagt ttctgatcca tacgggacgc gttcttgcac aaaggggagt
    18001 agcaattcat ggttatacca acaacctgaa aattcgtttt tgctttcaac tgactgataa
    18061 ataacatcgt tttcaggttc tttttacgca tcccctcaat acaaagatcc ggcgtaccgt
    18121 attgctgtgt tatgttcttt gctaaatctt ttatttcttt taatgttgcg tgatcctgca
    18181 tagtcattgt gactaatgtt aatttagtct gttcaagttt aagcgcatta aagacttcta
    18241 aattaattgt cgacgttaca attaaaagat gcttaatttt atgcaattca agcgcccgaa
    18301 taacaggaaa gatggccata gcatcgccaa tctgatcggg aatatggatg acaacaaagt
    18361 ctgttttttc aatattgaaa ttataagctt tataatcgta gtaactaaat gcaatacgtc
    18421 tcaacaatga tgctaaaaac atacctaacc tcgcctccct actggttata atgcaatgca
    18481 gtctatcaga ctcatcaggg tgccattttg tgcatatgcg gacttttatg tttcatatct
    18541 ctaacctgtg ggtcctctgc ttaatcctta aacaacacca gcaactcctg cgctttcatc
    18601 ttccatcgaa tttttcatgt tgccgctaat cagccataaa atcatttgca gatgcgctct
    18661 gtcgaggtag tctcataagg ttcgtttata gatcgacggc aatgtgagtt accttttcca
    18721 tactaattat aaaaagacag tacaaacagg atcattatgg actccacgct catctccact
    18781 cgtcccgatg aagggacgct ttcgttaagt cgcgcccgac gagctgcgtt aggcagcttc
    18841 gctggtgccg tcgtcgactg gtatgatttt ttactctatg gcatcaccgc cgcactggtg
    18901 tttaatcgcg agtttttccc gcaagtaagc ccggcgatgg gaacgctcgc cgcatttgct
    18961 acctttggcg tcggattcct tttccgtccg ctcggcggtg tcattttcgg tcactttggc
    19021 gaccgactgg gacgtaagcg catgttaatg ctgaccgtct ggatgatggg catcgcgaca
    19081 gccttgattg gtattcttcc ttcattctcg accattgggt ggtgggcacc tattttgctg
    19141 gtgacactgc gtgtcattca gggatttgcc gtcggcggcg aatggggagg cgcggcgttg
    19201 ctttccgttg aaagtgcacc gaaaaataaa aaagcctttt acagtagcgg tgtacaagtt
    19261 ggctacggtg taggtttact gctttcaacc ggactggttt cattgatcag tatgatgacg
    19321 actgacgaac agtttttaag ctggggctgg cgcattcctt tcctgtttag catcgtactg
    19381 gtactgggag cattgtgggt gcgcaatggc atggaggagt ccgcggaatt tgaacaacag
    19441 caacattatc aagctgccgc gaaaaaacgc atcccggtta tcgaagcgct gttacgacat
    19501 cccggtgctt tcctgaagat tattgcgcta cgactgtgcg aattgctgac gatgtacatc
    19561 gttactgcct ttgcacttaa ttattcaacc cagaatatgg ggctaccgcg cgaacttttc
    19621 cttaatattg gtttgctggt aggtggatta agctgcctga caattccctg ttttgcctgg
    19681 cttgccgatc gttttggtcg ccgtagggtt tatatcacag gtgcgttaat cggaacgttg
    19741 agcgcatttc ctttctttat ggcg
//
LOCUS       DAZPDU010000074        19662 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZPDU010000074 DAZPDU010000000
VERSION     DAZPDU010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19662
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.075"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..480
                     /locus_tag="ACHURS_002338"
     CDS             <1..480
                     /locus_tag="ACHURS_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFR1238049.1"
                     /translation="NDPEVRYIPNGGAVANLQVATSESWRDKQTGEVREQTEWHRVVL
                     FGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEILVKTTGTVQMLGRAPQQ
                     NAQAQPKPQQNGQPQSADATKKGGAKTKGRGRKAAQPEPQPQPPEGEDYGFSDDIPF"
     gene            538..771
                     /locus_tag="ACHURS_002339"
     CDS             538..771
                     /locus_tag="ACHURS_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HFR1238050.1"
                     /translation="MSEYFRIFQGLPDGSFTREQAEAVAAQYRNVFIEDDQGTHFRLV
                     VRQDGTLIWRSWNFEDGAGYWMNQYIRDFGILK"
     gene            830..2788
                     /locus_tag="ACHURS_002340"
     CDS             830..2788
                     /locus_tag="ACHURS_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFR1238051.1"
                     /translation="MPVTKCEPETTRKASRKSVKTQETALSALLAQTEEVSVPLDSLI
                     KSPLNVRTVPYSAESVSELAESVKGVGLLQNLVVHTLPGDRYGVAAGGRRLAALNMLA
                     ERGIIPADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEHLREAEGWEWCAGRMEPVGSCREDAGTYRSLPEPEAVLTEAEE
                     ERLNELMARYDALENQCEESDLLEAEMKLMRCIAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDVADDADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFGSGAYSKPAQVSLECKHYSLTSDAPSGKEGAAFLALMAEKVRL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDSLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEFHMKD
                     NRWVPGWMCSPRPQTDATERADNLADAA"
     gene            2843..3277
                     /gene="psiB"
                     /locus_tag="ACHURS_002341"
     CDS             2843..3277
                     /gene="psiB"
                     /locus_tag="ACHURS_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFR1238052.1"
                     /translation="MKTELTLNALQSMNAQEYEEIRAAGSDMRRNLTHEVMREVDAPA
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQLWMLVLVNGGGQPFAVV
                     QVQHIFTPVAISHTLALAATLDAQGYSVNDIIHILMVEGGQA"
     gene            3274..3993
                     /locus_tag="ACHURS_002342"
     CDS             3274..3993
                     /locus_tag="ACHURS_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFR1238053.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYATAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGEVCVLPLPSDAG
                     HRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPA
                     TVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRG
                     APVQVRALEQWMIPNKLENVI"
     gene            3990..4586
                     /locus_tag="ACHURS_002343"
     CDS             3990..4586
                     /locus_tag="ACHURS_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1238054.1"
                     /translation="MMKSDEKYQVPAWMRPLLPLLCNTGGNDPEELLNDTETTASANV
                     VRYVLIVAVRSQVDLLQLLYRKGLLRTEIPGGFSPEEAQALLDNLVRSHISKALSGER
                     MAARDRNADLAWIGQQLVDAAWFVRATLEAHGMSVGNESPPAPPETMPDIQTRELVML
                     IKRLASSLKAVKPDSCVVREAQDWLRDRKLVDITDILR"
     gene            5048..5548
                     /locus_tag="ACHURS_002344"
     CDS             5048..5548
                     /locus_tag="ACHURS_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFR1238055.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLTTFDDERDFFAACRAL
                     HQDETDPELMFQDYEGFPGNMASECHINWAWVEGFRQARDEGCEEAYRLWVDDTGETD
                     FDSFRDAWWGEADSEEAFAVEFVSDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
     gene            6281..6715
                     /locus_tag="ACHURS_002345"
     CDS             6281..6715
                     /locus_tag="ACHURS_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HFR1238056.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQQA
                     EALVEHGDIRCLGPRCDKPAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFREAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPE"
     gene            6810..7076
                     /locus_tag="ACHURS_002346"
     CDS             6810..7076
                     /locus_tag="ACHURS_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1238057.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRFRRQELAPDEIN
                     PDVLAMGGHIARRCSEQKRVRIPAMKVSEWGHLLRALEIERVCH"
     gene            7253..7642
                     /locus_tag="ACHURS_002347"
     CDS             7253..7642
                     /locus_tag="ACHURS_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CbiX"
                     /protein_id="HFR1238058.1"
                     /translation="MKSCFDTDKAFHMSFDEVTFNADRIRSKLELLSEELMPRCGLVY
                     ELYQRRLSAAIDNFVATLPTEQHLLVLELAREEFDYLSAEEIAEEIRRDSERGYCCHG
                     FERNCCPLGCGDLGDDCCDAADLMEEP"
     gene            7639..8517
                     /locus_tag="ACHURS_002348"
     CDS             7639..8517
                     /locus_tag="ACHURS_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFR1238059.1"
                     /translation="MSKKKNTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKTTGGEDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFENPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDTVSPEFMRALAERLPQHEDKLMTIAERLEQKGALEKALAIARQLQKMGMTPEQI
                     KQATGLSEAELKKIIH"
     gene            8576..8782
                     /locus_tag="ACHURS_002349"
     CDS             8576..8782
                     /locus_tag="ACHURS_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1238060.1"
                     /translation="MTIAERLIQKGFDEGFKESFKEGFKEGALEVAREIACRLRDMGW
                     PPERIQEATGLSGEELKKLFPDEQ"
     gene            complement(8813..9064)
                     /locus_tag="ACHURS_002350"
     CDS             complement(8813..9064)
                     /locus_tag="ACHURS_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFR1238061.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAEDV"
     gene            complement(9098..9294)
                     /locus_tag="ACHURS_002351"
                     /pseudo
     CDS             complement(9098..9294)
                     /locus_tag="ACHURS_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753860.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9643..10491
                     /locus_tag="ACHURS_002352"
     CDS             9643..10491
                     /locus_tag="ACHURS_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFR1238062.1"
                     /translation="MNQTLPTADLNTAGTTDVIPSVAIDRIIAQRNEGIALFMQAMEC
                     LATARKILLDASGDIFLYGFEDCVTDSVRCMDKPEEAKKNITRLADRKIWDRLMTDTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNASKMQTFEQGLIDVYRKLSW
                     DYRTNNPCRLGKKIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEARNVPDFRN
                     SIGAQYGEFLGNGDNVGKLLEGEYFTVRGYQKGTVHIVFKRPDLVEKMNDIIARHYPG
                     ALPPRV"
     gene            complement(10577..10912)
                     /locus_tag="ACHURS_002353"
     CDS             complement(10577..10912)
                     /locus_tag="ACHURS_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HFR1238063.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     VLVRTKGYTLADVRDTLKNAGYEVGEKALRDIIREAESKKLSRRSSSKTAPKKTDSGS
                     KDGIDMNNN"
     gene            11145..11477
                     /gene="mobA"
                     /locus_tag="ACHURS_002354"
     CDS             11145..11477
                     /gene="mobA"
                     /locus_tag="ACHURS_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HFR1238064.1"
                     /translation="MSEKKTRSGSEKRQKNVLIAVRFSPEEAEIVKEKAEKNGLTVST
                     LIRKTVLGKQINARIDEDFLKELMRLGRLQKHLFVEGKRTGDKEYAEVLVAITELANT
                     LRRNLMGR"
     gene            11489..14209
                     /locus_tag="ACHURS_002355"
     CDS             11489..14209
                     /locus_tag="ACHURS_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFR1238065.1"
                     /translation="MNAIIPKKRRDGKSSFEDLIAYTSVRDDVPEDELRPDTEVKGEV
                     PHRNRFRRLVDYATRLRNEKFVSLIDVMKDGSQWVNFYGVTCFHNCLSLESAAEEMQK
                     AADLAHFSKDDTDPVFHYILSWPAHESPRSEQLFDCVRHTLKSLELSKHQYVAAVHTD
                     TDNLHVHVAVNRVHPETGYINCLPWSQEKLSRACRELELKHGFAPDNGCFVHAPGNRI
                     VRKTALVRERRNAWRRGKKQTFREYIAQMSIAGLREEPAQDWLSLHKRLASDGLYITM
                     QEGELVVKDGWDRAREGIALSSFGPSWTAEKLGRKLGEYQPVPTDIFSQVEAPGRYDP
                     EAINVDIRPEKVAETESLKQYASRHFAERLPAMARNGELKSCLAVHRTLAEAGLWMGI
                     QHGHLVLHDGFDKQQTPVRADSVWPLMTLDYMQDLDGGWQPVPKDIFTQVVPGERFRG
                     HSIGSQPVSDYEWYRMRMGTGPQGAIKRELFSDKESLWGYGLIQCRSLIEEMIADGDF
                     SWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEPQAGPFE
                     IAAADIFERVKPECRYNPELAASDEVEPGFRRDPEIRRERREARAAAREDLRARYLAW
                     KEHWRKPDLRYGERLREIHAACRRRKAYIRVQFRDPQLRKLHYHIAEVQRMQALIRLK
                     ESVKEERLSLIAEGKWYPLSYRQWVEQQAAQGDRAAVSQLRGWDYRDRRSRNKDKRRT
                     TNVDRCVVLCEPGGTPLFNNVAKLEARLQKNGSVHFRDTRTGKNVCTDYGDRVVFYHH
                     TDRNELAEKLNLIAPVLFSRNGKLGFEPEGSYQQFNDVFAEMVAWHNAAGITGNGHFT
                     ITRPDVDLHRQRSEQYYREYIRQQTRLSESHDDNYTLRQEKTWEPPSPGM"
     gene            complement(14430..16730)
                     /gene="trbC"
                     /locus_tag="ACHURS_002356"
     CDS             complement(14430..16730)
                     /gene="trbC"
                     /locus_tag="ACHURS_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000077650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugative transfer protein TrbC"
                     /protein_id="HFR1238066.1"
                     /translation="MSQHHVNPDLIHRTAWGNPLWNALHNLNIIGLCLAGSIITALIW
                     PLALPVCLLFTLVTSVIFTLQRWRCPLRMPMTLSLDDPSQDRKVRRSLFSFWPTLFQY
                     EADETSPARGIFYVGYRRINDIGRELWLSMDDLTRHVMFFATTGGGKTETTFAWLLNP
                     LCWGRGFTFVDGKAQNDTTRTIWYLSRRFGREDDIEVINFMNGGKSRSEIIQSGEKSR
                     PQSNTWNPFAFSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGK
                     TMSLQMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDMSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFTTFAETFGDVFAADAGDIDIRDSIHSDRILIVMIPALDTS
                     AHTTSALGRMFVTQQSMLLARDLGYRLEGTDAQTLEVKKYKGSFPYICILDEVGAYYT
                     DRIAVEATQVRSLEFSLIMTAQDQERIEGQTSATNTATLMQNAGTKFAGRIVSDDKTA
                     RTVKNAAGEEARARMGSLQRHDGVMGESWVDGNQITIQMESKIDVQDLIRLNAGEFFT
                     VFQGDVVPSASVYIPDSEKSCDSDPVVINRYISVEAPRLERLRRLVPRTVQRRLPTPE
                     HVSSIIGVLTAKPSRKRRKNRTEPYRIIDTFQRQLATAQTSLDLLPQYDTDIESRANE
                     LWKKAVHTINNTTREERRVCYITLNRPEEEHSGPEDIPSVKVILNTLLPLEILLPVAE
                     NNKSQTINNDHPQKSCGAQPTTDEKY"
     gene            complement(16711..17835)
                     /locus_tag="ACHURS_002357"
     CDS             complement(16711..17835)
                     /locus_tag="ACHURS_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HFR1238067.1"
                     /translation="MTIHYTATLNNKHLSLTALYPDGNREVIAILPVTELGALINRNN
                     RLMHLSFSSPFREITETVLVDTVDAERPGHHIGRMLSAIDACLDAEYAGKGKHPSRKY
                     GFWRTLMCGFIAGIALPATVAGYHWMKAEPPSTLAYPKSQEWRAEPVTDTETPRPHTE
                     HHVNSIPARPAPTQQEPSSAPAPTAVTEEQHVQAAEARRNLATVLKRNADRGMFTINL
                     STGHERTLYAFLDPACINCRLLEPALKQLAREFSVVIYPVSVIGGESSTKRTAPLLCE
                     PDSQKRATGWHQLYSADSGMAPTGDAPAAQDGQCLTAARAAIDVNNQAFRQFGFGGTP
                     WVLSDTGWHLPSGILKDATSVRLFLKATDSEEYHNEPAPR"
     gene            complement(17832..19130)
                     /gene="trbA"
                     /locus_tag="ACHURS_002358"
     CDS             complement(17832..19130)
                     /gene="trbA"
                     /locus_tag="ACHURS_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbA"
                     /protein_id="HFR1238068.1"
                     /translation="MSYQHSPTTEDPMNIWYIVGTICLLFALLIWLFLPDIVFATCLI
                     LHTMWGMTDWGPFHHFAAPRYNLLAITANNAATITFGQWLDVMSRTVGILWLILLPMT
                     FGFLWMWFHHPAQPRFTRRPLNIHTLPHIFSALSPAIAPVLADGDNNRLFHGQKRPER
                     RVALTPEVFVEQNNLIRNMQLDVASTRQCFMAQLGQPLTSWKDMAPHEKALFAIFGLQ
                     FFLGDRKAAVALMNNLNLSCRLKSKRDQGRFSTPVYSLARNAFIRVIKTEGAQQWLRQ
                     HRYVRSGLVWLYAHDLRLTPPNWLWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARA
                     ENEASRLGLPCPEPCVEEAIEGLRRDMLGLGLIWDEPQPDRDRKRQIRTRWSLTDDVI
                     PRRHDNDEDTDTGETTETTETTETTETTEKQHPADKEKEQ"
     gene            19468..19614
                     /locus_tag="ACHURS_002359"
     CDS             19468..19614
                     /locus_tag="ACHURS_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1238069.1"
                     /translation="MSGDLPSHQSIGTMDLCYSKTWPDKQVVLRLVLWPEEVSFQLTM
                     KELL"
BASE COUNT         4450 a         4920 c         5760 g         4532 t
ORIGIN      
        1 aatgatccgg aagtccgtta catccccaac gggggcgcag tggcaaacct gcaggtggct
       61 acgtcagaaa gctggcgcga caaacagacg ggggaggtgc gggagcagac ggaatggcac
      121 cgcgtggtgc tgttcggcaa gctcgcggaa gtggcaggtg aatatctgcg caagggtgcg
      181 caggtctaca tcgaaggtca gcttcgcacc cgtagctggg aagataacgg tatcacccgt
      241 tacgtcactg aaattcttgt taagaccacg ggcaccgtgc agatgctggg acgtgcacca
      301 cagcagaacg ctcaggcgca accgaagcct cagcagaatg ggcagccaca gagtgctgac
      361 gcgacgaaaa aaggtggcgc gaaaacgaaa ggccgtggac gtaaggccgc gcagccagag
      421 cctcagccgc aaccgccgga gggggaggat tacgggtttt cagacgacat cccgttctga
      481 tcgggctgac tgtgacaacc gccccgccct gtgcggggca tcaccggaga tatgaggatg
      541 agcgaatatt tcagaatatt tcaggggctg cctgacggct cctttacccg cgaacaggct
      601 gaagccgttg ccgcacagta ccggaatgtc tttatcgagg atgatcaggg aacgcatttt
      661 cgcctggttg tccgtcagga tggcacgttg atctggcgct cctggaattt tgaggacggt
      721 gccgggtact ggatgaacca gtacatcagg gatttcggga ttcttaagta agagaggtgc
      781 cggacgcgcg aacgtccggc aggacataag caattataag gggatgatta tgcctgtaac
      841 gaagtgtgaa ccagaaacca cccgcaaagc aagccgtaaa tctgtaaaaa cgcaggaaac
      901 tgcactgtct gccctgctgg cgcagacgga ggaagtgagc gtgccgctgg attcactgat
      961 taaatcaccg ctgaatgtgc gcacggtgcc gtattctgcg gagtccgtca gtgaactggc
     1021 ggagtccgtt aagggcgttg gcctgttgca gaatctggtc gtgcataccc tgcctggtga
     1081 ccgttacggt gtcgccgcag gtggtcgccg actggcagca ctcaacatgc tggcagagcg
     1141 tggcatcatt ccggctgact ggcctgtacg cgtgaaggtt attccgcagg agctggcgac
     1201 ggctgcatca atgactgaaa acggtcatcg tcgggatatg caccctgccg aacagattgc
     1261 cggatttcgt gcaatggcgc aggaaggcaa aacgcctgca caaatcggtg atttgctggg
     1321 ctattcgccc cgccacgttc agcgaatgct gaaactggca gaccttgcgc ctgtcatcct
     1381 cgatgcgctg gcagaagacc gcatcaccac agagcactgt caggcgctgg cgctggagaa
     1441 cgacaccgcg cgtcaggtgc aggtgtttga agccgcctgc cagtcgggat ggggcggtaa
     1501 accggaagtg cagaccattc gtcgtctggt gaccgaaagt gaagtggcgg tggcggggaa
     1561 cagtaaattc cgcttcgtgg gggctgatgc cttctcgcca gacgaactgc gcaccgattt
     1621 gttcagcgac gacgagggtg gctatgtgga ctgtgttgcg ctcgatgctg ccctgctgga
     1681 aaaactccag gctgtcgctg aacaccttcg ggaagccgaa ggctgggaat ggtgcgccgg
     1741 acgtatggag cctgttggtt cctgccgtga ggatgccgga acataccgca gtctgccgga
     1801 gccggaagcg gtgctgacgg aggcagaaga agaacgcctg aacgaactga tggcacgtta
     1861 cgacgcgctg gaaaaccagt gtgaggaatc cgacctgctg gaagcagaaa tgaagctgat
     1921 gcgctgtata gcgaaggtca gggcgtggac gccggaaatg cgtgccggaa gcggtgtggt
     1981 ggtgtcctgg cgttacggta atgtgtgtgt ccagcgtggt gtgcagttgc gcagtgagga
     2041 tgacgtggct gacgacgctg accgcacgga acaggtgcag gaaaaagcgt cagtggagga
     2101 aatcagcctg ccgttgctga cgaaaatgtc ctcagagcgc acgctggcag tccaggcagc
     2161 actgatgcag caaccggaca aatctctggc actgctggca tggacgctct gcctgaatgt
     2221 gtttggcagc ggagcgtaca gtaaaccagc acaagtcagc ctggaatgta aacattattc
     2281 gctgaccagc gatgcgccgt cagggaagga aggtgccgca ttcctggcgc tgatggcaga
     2341 aaaagtccgt cttgcagccc tgctgccgga gggatggtca cgggacatga cgacgttcct
     2401 gtcactcagt caggaggtgc tgttatccct gctcagcttc tgtaccgcgt gcagtcttaa
     2461 cggtgtccag acccgcgagt gtggtcacac gtcacgcagt ccgcttgact cgctggaaag
     2521 cgccatcggc tttcacatgc gtgactggtg gcagccgaca aaagcaaact tcttcggaca
     2581 cctgaaaaag ccgcagatta tcgcagccct gaatgaggca gggctgtccg gtgccgcacg
     2641 ggacgcggag aagatgaaga aaggcgatgc ggctgaacat gcagagttcc atatgaaaga
     2701 caaccgctgg gtgcctggct ggatgtgttc accacgtcca cagacggatg ccactgaacg
     2761 cgccgataac ctggctgatg ccgcctgatg aacaaccaca ccgccccgcc ggagacgggg
     2821 cggcagcaag ggagataccg tgatgaaaac tgaactgaca ctgaatgccc tgcagtccat
     2881 gaatgcccag gaatatgaag aaatccgtgc tgcgggaagc gatatgcgcc gtaatctcac
     2941 tcacgaggtg atgcgtgaag tggacgcacc ggctaactgg atgatgaacg gagagtatgg
     3001 cagcgagttc gggggctttt tccccgtcca ggttcgtttc acgccagccc acgaacgttt
     3061 ccacctggca ttatgttcgc cgggagacgt ctctcagctc tggatgctgg ttctggtgaa
     3121 tggtggtgga cagcctttcg ccgtcgttca ggtgcaacat atcttcacgc ctgtcgcgat
     3181 cagtcacacg ctggcgcttg ccgcgacact ggatgcgcag ggatacagtg ttaacgacat
     3241 catccatatc ctgatggtag aaggaggtca ggcatgagcg cacgttcacg ggcactgatc
     3301 cccctcagcg cagaacaaca ggccgccatg caggcggtgg ctgtcacaga acaacgtcgt
     3361 cgtcagggac gcacactttc agcatggcct tatgccaccg ctttctttcg ctgcctgaat
     3421 ggcagtcgcc ggatttcgct gaccgatctc cgcttttttg cccctgcgct gacgaaggag
     3481 gaatttcatg gcaaccgtct cctgtggctg gctgccgtgg ataaactgat tgaaagtttt
     3541 ggggaagtct gtgttcttcc cctgccatcc gatgcggggc accgtctgtt cccgtccgtg
     3601 ccttttcgtg aaggtgagcg tcgtcgtcag aaaaccacgc tgacagagca gaaatacagc
     3661 cgccagcggg aacgtgaggc agaacgacgg gaactggaat accagacatg ttttgctcag
     3721 gcgcagattg accttgcgtt tcatactccc gccacggtcg gaagctggtt gtcccgctgg
     3781 tctggtgttg ttgaggagca tgatctggaa acgattttct gggggtggtg cgggcgtttt
     3841 ccatcactgt catcatttga ccggtttttc tggcaggagg aaccactctg gcggctgatt
     3901 tttgaagccg gtgaggccgg tcgtggtgca ccggtacagg tacgtgcact tgagcagtgg
     3961 atgatcccga acaagctgga gaacgtaata tgatgaaatc agacgaaaaa taccaggttc
     4021 ccgcgtggat gcgacctctg ttgccgttgc tctgcaacac cggggggaac gatccggaag
     4081 aactgctgaa tgatacagaa accactgcca gtgcgaatgt tgtccgttat gtactgatag
     4141 ttgctgtgcg gtcgcaggtt gatctgctgc agcttctgta caggaaaggg ctgttgcgca
     4201 cagagatacc aggtggcttt tcaccggaag aagcgcaggc actcctggat aatctggtgc
     4261 gcagccatat cagcaaggcg ctgtcgggcg agcgaatggc agcccgtgac agaaatgccg
     4321 atctggcctg gattggacag caactggtcg atgccgcctg gtttgtccgt gccacactgg
     4381 aagcgcatgg gatgagcgtc ggaaatgaga gtccccctgc tccgccggag acaatgccgg
     4441 acatacagac acgggaactg gtgatgttga tcaagcgact ggcatcatcg ctgaaagcgg
     4501 tgaaaccgga cagttgtgtg gtgcgtgaag cgcaggactg gctgcgcgac agaaaacttg
     4561 tggatatcac agatattctc cggtgaacca ggcatgtata ttttacgcag ggacacgccg
     4621 atcatgatcg tttcatatca ggggtggtct ggaggtcatg cggtgtgctc cctgctctcg
     4681 ccggaataag gacgtcgccg ccggctccgc ttttacccgg ccatgcgggg catggccttg
     4741 tgggttttca gttatgtggc cgccgtgtcg tgtgcgggct gtgccgtgcc tccatcgtag
     4801 ccgggcttct gcgggcggga tgcaaggtaa gatgaaccgc tgcggtcacc cggtccctcc
     4861 ttcccgtctg ccgtgatttt ccggtcgttc acccgctcag atttcgcggt ctcaccctcc
     4921 aactcccgcc agccgtgtgc gtcgggctgc ggcttccctt gcgcccttgc atccctgcag
     4981 gcccggcaaa gatggaggca cggcaccgcc cggtgtcgca gaacatgtga ctatggagga
     5041 ttcgggaatg tctgttgttg cacctgctgt atacgttgga acctggcaca aatacaactg
     5101 tggaagcatc gctggccgct ggtttgacct gacaacgttt gatgatgaac gcgacttttt
     5161 cgccgcctgc cgtgcccttc accaggatga aaccgacccg gaactgatgt ttcaggatta
     5221 tgagggattc ccggggaata tggcctctga atgccatatc aactgggcct gggttgaagg
     5281 cttccgccag gcgcgggatg aaggctgcga agaggcttat cgtctctggg tggatgatac
     5341 cggtgagacg gattttgaca gcttccgcga tgcctggtgg ggcgaggctg acagtgagga
     5401 ggcttttgcg gttgagttcg tcagtgatac cggcctgctg gctgacgtgc cggagacggt
     5461 ggcgctctat tttgactatg aggcgtatgc gcgggattta ttcctggact ccttcacctt
     5521 tattgacggt catgtgttcc gtcggtgatt tactccccgc tccggcgggg ggatggcctg
     5581 ccaggccccc ggaaaaccct ggcaggctta cgcctgcctg aaatacagga gggccgcgcc
     5641 tgcggcgcta cccttcctgc ctgtcatagt gtgaggcatt tttctgtttt gtcgtttctg
     5701 ttgttctgac tcccgggggc ggggacaccc ttcggctccc ggctgcctgg cggcatccgg
     5761 tcgtcaggcc cgactccgac aggcagctgc tggcgcaccc gccagtctcc gccgaacccg
     5821 ctggcgcggt ttcgggctgc cgctacggtc cgatgtctga aaaaccaggc ttgcagcgac
     5881 cgttccggtc gcgccgcctc tgtcgcgccg tgacctgcgc tccctgcaca cctgacggtg
     5941 tgtaaggaaa ctgcgcctga tggcgcagtt gtctgccctt ccgccgcccc ccctgcggcc
     6001 tgtttccccg ctgtggactt tccgggggct gaagcggcca caacacgttt cagccgtcca
     6061 ggggagagat gtaccagcca caaaactttt tgccgcaagc gtcaaaaact tttgcgtctg
     6121 cccccggaca actgccccgt gccgtggcag tcgctgcccc atcggaaagg tccactgcgg
     6181 ggagaaaccc cgaagaagtg gacggcagaa ggggctttcg cccctccggg ggagcaaccg
     6241 gtcggcccgg cgacctgatt cagcagaagg agacgttttc atgacagtca gcagcacgat
     6301 ttccgttttt tgccgcgacg gggtgttccg taccgtttac tgccacctgc acggtgagcc
     6361 gacctggaac ggtcgcatcc tgcataccca ctatgccacc ggtcagcagg ccgaagccct
     6421 ggttgaacac ggtgatatcc gttgcctcgg tccccgttgc gacaaacccg ccgggcatac
     6481 gcttcagaac ccggtggacg gtgtgacggc ttattacgga cgtgacagtg gtttccggat
     6541 ggacagtgag gcgcgtgagt accgttcttt cagggaggcc atcgccacgg aaagcactga
     6601 agaggtgcgc ttccattatg tgttcatcga cggctactgg aaggtgatgt accgcacgcc
     6661 ggaaggctgg aagatgaaag cgctcgcgct ggcactgcgt cgctgtccgg aatgaaaaaa
     6721 aaagcagggc gataaaccct gcctttctcc gctggcgctt ccccgtacgg aagtccggca
     6781 tctcattcac taactatgga gattatgcca tgagaccatc aattatcttc gcaaccgccg
     6841 agtatgtaaa gcgtctgcga gaagagtgcc tgcgggagaa caaacccctg caccgccata
     6901 cccgcttcag acgccaggaa ctggcaccgg atgagattaa cccggacgtc ctggcgatgg
     6961 gcggtcatat cgccagacgc tgcagtgagc agaagcgggt acgtatcccc gccatgaaag
     7021 tcagcgaatg gggccacctg ctccgcgcgc ttgaaattga acgggtctgt cactgattaa
     7081 cccctgccgt ctgcctgtgc aggtggcagg atgtgttcag gtactttacc aggaggaacc
     7141 gtgagcaaga tgaaaaacat cacgacgtct gtgttgcatg gtgtctccac aaaaaacaga
     7201 aaagggaaac agattctctt tcattcaggg cccgggaggg ctggagataa caatgaagtc
     7261 ttgttttgat actgacaaag cttttcatat gtcttttgat gaagtcactt tcaacgctga
     7321 cagaattcgc tcaaaactgg aactattatc cgaagaacta atgccccgtt gtggtcttgt
     7381 ttatgagcta tatcagcgac gtttgagtgc tgctattgat aattttgtgg ccacattgcc
     7441 gacagaacaa catctactgg tcctggaact ggcccgtgag gagtttgatt acctttccgc
     7501 agaagagatt gctgaagaaa tacgccgtga ctcagaacgc ggctattgtt gtcatggctt
     7561 tgaacgcaat tgctgtcctc tcggttgtgg ggatttgggc gatgattgct gcgatgctgc
     7621 tgatttgatg gaggaaccgt gagtaagaag aaaaacacca caacgcccac gccgcatgat
     7681 gccgcgttcc ggtcgttcct ggcgaatccc gacgtcgcca gagattttct ggaactgcat
     7741 cttcctgcgg agtaccggca gttgtgcgac ctgtccacgc tgaagctgga acccgccacc
     7801 tttgtagagc cggacctgca tcagtacgcc agcgatatcc tctggagcgt gaaaaccacc
     7861 gggggtgaag acggctatgt ttatacgctc atcgagcacc agagcacaga aaatctgtac
     7921 atgccttttc gcatgttacg ctacagtgtg gcggcgatgc agagacatct ggagcagcac
     7981 aaaacgttgc cactggtgat tccggtgctg ttctatcacg gtgagcgcag cccgtacccg
     8041 tacagcatga actggctgga ctgttttgag aatccggcac ttgcggctaa aatatacaca
     8101 aagccgtttc cgctggttga tatcactgtc gttgatgaca atgaaatcat gaaccatcgc
     8161 cggatggccg cactgacgct gctgatgaag catatccgcc atcgtgacat gatggagctg
     8221 ctggacaaac tcccgcaggt catggtggag atttcagacg agcaggtgcg tgttctgatt
     8281 cattacatcg ttaacgcagg ggacactgta tcaccggaat ttatgcgggc gctggccgag
     8341 cgcctgccgc agcatgagga taaactgatg acaatcgcag aacgtcttga gcagaaaggt
     8401 gcgcttgaaa aggccctggc cattgcgcgt cagcttcaga aaatggggat gacacctgag
     8461 cagattaagc aggctaccgg actgtccgaa gctgaactga aaaaaatcat tcactgatga
     8521 gagcatcaca gcccggcaga atgccgggct tttttgccag caggggcaga gcagtatgac
     8581 tattgcagaa cgtcttatac agaaaggttt cgacgaaggt tttaaagaaa gttttaaaga
     8641 aggctttaaa gaaggggcgc ttgaagtggc gcgggaaata gcctgccggc ttcgggatat
     8701 gggctggccg ccggaacgga ttcaggaggc aaccggactt tccggtgaag aactgaaaaa
     8761 actgtttcct gatgagcagt agcctggcat tcagccaggc tgtgaattac gctcacacat
     8821 cctctgccgt ttcccccagt tcattttcca gtcgcctgat gatatctgcc ttgcgcatcc
     8881 cctgcatgtg aggcagcagg tcacggactg aaagtgcctg ggtggtcctc cagacggtga
     8941 catccacctg ctccgcattt tcccaggcgt gccatgttct ctgtgtcacg ccaaattctc
     9001 tggccgcttt ttcgcgtgac cagagcatgc tttttcgcca gattcgcagt tcccagccgg
     9061 tcataaagtg ccttttaaaa agtgaaaaaa cttcacttta ttttatcatc ataatcacca
     9121 ggtcagcgcg ataaataagc gtgaaaaagt gaagaaattt cacttttttt agttgtagat
     9181 gtcgtgctgt tttctctgtt ttcacctgtg cgggagtggg tgtcagtgac caccacatgc
     9241 cattgttgtg ccgtcgtttt tcttcagtcg cgccggatgc agcgctccct gcacacctta
     9301 cggtgtgtaa ggccactgcg cctgatggcg cagctgtctc tgcctgtctg ccgccgcact
     9361 gcggcctgtt ctccgcagac ggactttccg ggggtacata catgaccaca tcccgtttca
     9421 gtcgtccagg ggagagatgt accagccaca aaactttttg ccgcaggcgt caaaaacttt
     9481 tgcgtctgcc cccggacaac tgccccgtga cgtggtgagc gtcccccgtc ggaaaggtcc
     9541 actgcgggga aatccccgaa gacccggacg gcagaaggag cttcgctcct ccgggaaatg
     9601 acctggctga cccggcagtc gtgacgatga ggagacaatg tgatgaatca gactttaccc
     9661 actgctgacc tgaatactgc cggcacgaca gatgttattc cgtctgtggc tatcgaccgc
     9721 atcatcgcgc agcgtaacga aggtattgca ctgttcatgc aggcgatgga atgcctggcg
     9781 acagcgcgca agattctgct cgatgcgtca ggtgatattt ttctttacgg gtttgaagac
     9841 tgcgtgactg actccgttcg atgcatggat aaaccggaag aagcaaaaaa gaatatcacc
     9901 cgtcttgccg accggaaaat ctgggatcgc ctgatgacgg atactggcat gtacaccttc
     9961 atgagttcat gccagcgtga tgagtggaac agccagctga tgagcgacac ctgtcctgaa
    10021 atcaccctgg acaatgtact ggcaacattc cgccatctga atgccagcaa gatgcagaca
    10081 tttgaacagg gactgattga tgtctaccgg aaattgtcat gggattacag aaccaataat
    10141 ccctgccgtc tgggtaagaa aatcattatt gaaaacctgc tgtaccgctg gagtaacgga
    10201 cgtgtgacgc tggactgcag cggacgggag gcactggatg acctggtacg tccgttttat
    10261 ctgctggagg cgcgcaacgt tcctgacttc aggaacagca tcggggcgca gtatggtgaa
    10321 tttctcggga acggcgacaa tgtcggtaag ctattagaag gggaatattt tacggtgcgt
    10381 ggctaccaga aagggaccgt acacattgtc tttaagcgtc ctgaccttgt tgaaaaaatg
    10441 aatgatatta ttgcacggca ttatcctggt gcattgccgc cacgagtctg attaaacaga
    10501 aaagccctgg tatttatgcc ggggcttttt tttctgtaat catcataatc tcagaacacg
    10561 atgtgacatt gtcacatcaa ttgttgttca tatcgatgcc atctttactg cctgaatctg
    10621 tctttttagg tgctgtttta gaggaagaac ggcgggatag ttttttcgat tctgcttccc
    10681 gaataatgtc acgaagtgcc ttctctccta cttcataccc tgcatttttg agcgtgtcac
    10741 gaacatctgc cagtgtataa ccctttgtcc ttaccaggac aataatgtca tcacgaatgg
    10801 catcaagaaa atcacgcaat gttttccgtt gagcggtgag gtctggcaat tccgataaag
    10861 cagcttttgc cagctggatg tcatcatctg agtaaaattt tttagacgcc attggcactt
    10921 tctctccgtt taatgttgaa gcctgatttt atcatcaaaa actgactcct ggcagggctg
    10981 cgccctgcct ctgccgtctg cataagacta tgatgcacaa aaataacagg ctataatgga
    11041 ctgaaaaacg ggacaggatg tgtaatcgta taaccgcgca cgcacggcta tacgattacc
    11101 acctggtcag ggcttagccc cgacaccccg gtaaggagca gaccatgagt gagaagaaaa
    11161 cccgaagtgg cagtgagaaa cggcaaaaga atgtcctcat tgctgttcgt ttttcaccag
    11221 aagaggctga gattgttaaa gaaaaggcag aaaaaaacgg actgacagtt tcaactctga
    11281 taagaaaaac tgtgctggga aaacagataa atgcccgcat tgacgaagat tttttgaaag
    11341 agctgatgcg tcttggtcgc ctgcagaaac atctgtttgt ggaaggaaaa cgcacgggag
    11401 ataaagagta tgcagaggtt ctcgttgcta ttacagagct tgctaatact ctgcgaagaa
    11461 atttaatggg acgctgagat aatcgctgat gaatgccatc attccgaaaa aaagacgtga
    11521 cggaaagtct tcctttgaag acctgattgc gtacacctca gtgcgggatg atgtgccgga
    11581 agatgaactc agaccagaca cggaagtgaa aggtgaggtt ccgcaccgga atcgcttcag
    11641 acgtcttgtt gattatgcaa cacgtctgcg taatgaaaaa tttgtttccc tgattgatgt
    11701 gatgaaagac ggcagtcagt gggtgaattt ctatggtgtg acctgctttc ataactgttt
    11761 atctcttgaa tctgcggctg aagaaatgca gaaagcagca gaccttgctc atttttccaa
    11821 agatgataca gatcctgttt ttcactatat tctctcctgg cctgcgcacg aaagtccgcg
    11881 ttctgagcag ctttttgact gtgtgcgtca caccctgaaa tcccttgagc tgtcaaaaca
    11941 tcagtatgtg gcagccgttc atacagacac ggataacctg catgtacatg ttgcagtgaa
    12001 tcgtgtccac cctgaaaccg gctatatcaa ttgccttccg tggagccagg aaaaactcag
    12061 tcgtgcatgc cgtgaacttg aacttaagca cggattcgcg ccggataatg gttgctttgt
    12121 acatgcaccg ggcaatcgta ttgtgcgcaa aacagcgctg gtacgtgagc gccgtaatgc
    12181 ctggcgacgc gggaaaaagc agacgttcag ggaatatatt gctcagatgt cgattgccgg
    12241 gctgcgtgaa gagccagctc aggactggtt atctttgcat aaacggctgg ccagtgatgg
    12301 tctgtatatc acgatgcagg aaggggaact ggtggtgaag gatggctggg acagagcgcg
    12361 ggaaggtatt gcactcagtt cgttcggacc gtcatggaca gctgaaaaac tggggcggaa
    12421 actgggggaa tatcagcctg taccgacaga tattttcagt caggtggagg cacccggtcg
    12481 ttatgatccg gaagccataa acgttgatat ccggccggaa aaagtggctg aaacggagag
    12541 cctgaagcag tatgccagtc gtcattttgc cgaacgctta ccggcaatgg cgcgcaatgg
    12601 tgaactgaaa agctgcctgg ctgtacaccg tactctggca gaggcgggat tatggatggg
    12661 gattcaacac gggcatctgg tactgcatga tggatttgat aaacaacaga caccagtacg
    12721 tgcagacagt gtgtggccgt taatgacgct ggattatatg caggatctgg atggtggatg
    12781 gcagcctgta ccgaaggata tttttactca ggtcgttccc ggagaacgat tccggggaca
    12841 cagtattggc tctcagcctg tcagtgatta cgaatggtat cgtatgcgga tgggcacagg
    12901 tccgcagggg gctataaagc gtgaactgtt ttctgataag gaaagtctgt ggggatacgg
    12961 ccttattcag tgcaggagct tgattgaaga gatgatagct gacggggatt tcagctggca
    13021 ggcatgtcat gagatgtttg cgcgtaaagg gcttatgctg cagaaacagc atcatggtct
    13081 tgtgattgtg gatgcgttta atcatgagct gacaccggtg aaggccagca gtatccatcc
    13141 tgatttgacg ttatcccgtg ccgagcctca ggccgggcca tttgaaattg cggctgcaga
    13201 tattttcgaa cgggtgaagc ctgaatgtcg ttacaatccg gaacttgctg ccagtgatga
    13261 agtggaaccc ggcttccggc gcgatcctga gattcgtcgt gagcgccgtg aagcccgtgc
    13321 agctgcgcgg gaagatttgc gtgcgcgtta tcttgcatgg aaggagcact ggcgtaaacc
    13381 cgacttacgt tacggagaac ggttacggga aattcatgct gcctgccgtc ggcgcaaggc
    13441 gtatatccgt gtgcagttcc gggatccaca attgcgtaag ctgcattacc atattgcgga
    13501 agtgcagcgt atgcaggcgc tgatcaggct gaaggagagt gtgaaggaag agcggttgtc
    13561 actgattgcg gagggtaagt ggtatccttt gtcctaccgg cagtgggtgg aacaacaggc
    13621 ggctcaggga gacagggctg cagtatcgca gttacgtggc tgggattatc gtgatcgtcg
    13681 ctcgcgtaat aaggataaaa gacgcacaac taatgttgat cgttgtgtcg tactctgcga
    13741 acccggagga acgccacttt ttaataatgt tgcaaaactt gaagctcgtc ttcagaaaaa
    13801 tggaagtgtt catttcaggg atacgcggac aggaaaaaat gtctgtacgg attacggaga
    13861 tcgggttgtg ttctaccatc acacggaccg aaatgaactg gcagagaagc ttaatttaat
    13921 tgcgccagtt ctgttcagcc gcaacggaaa gcttggtttt gagccggaag gttcatacca
    13981 gcagtttaat gatgtgtttg cagaaatggt ggcctggcac aatgcagcgg gaataaccgg
    14041 aaacggacac ttcaccatta ctcgtccgga cgtggatttg caccgtcaac gcagtgagca
    14101 gtattaccgg gaatatatca gacagcagac ccgtttatcg gagagccatg atgataatta
    14161 tacgttacgg caggagaaaa catgggagcc accatcacct ggaatgtaag cccggataag
    14221 tgatggtgca aagattcagc ccaaaaacgg caggaacctt cgtcaataag ggcgggagta
    14281 gtcactgaat tgattttata cccggttaac tctgaattaa gggccgtgtc tgcggccctt
    14341 atccagtgcg ccgtaaaacc ccggacttag tgaacagggt tttacagtct gattgacaga
    14401 tatggtcacc agaaaatatt tgtattcctt cagtattttt cgtcagttgt tggttgtgct
    14461 ccacatgatt tctggggatg atcattattt attgtctgcg atttattatt ttcagcgacc
    14521 ggtaacagta tctccagggg gagcagggta ttcagaatca ccttcactga aggtatatct
    14581 tcaggaccgg agtgttcctc ttccggacga ttcagtgtga tataacatac ccggcgttct
    14641 tccctggttg tgttattgat agtgtgcaca gcctttttcc acagttcgtt tgcccgggat
    14701 tcgatatctg tatcatattg cggaagcaga tccagtgatg tctgcgctgt tgccaactgg
    14761 cgctggaatg tatcaatgat acggtaaggc tctgtacggt ttttacgacg tttacgggat
    14821 ggttttgctg tcagtacacc gatgatgctg ctgacatgct ccggtgtggg gagcctgcgc
    14881 tgcactgtcc tggggacgag acggcggaga cgctccagcc ggggggcttc aacggaaata
    14941 tagcggttga tgaccaccgg atcactgtcg caacttttct cgctgtccgg aatatacaca
    15001 gaggctgacg gcaccacatc cccctggaaa acggtaaaga actcaccggc attcagtctg
    15061 atcaaatcct gcacgtcgat tttgctttcc atctggatgg tgatctggtt accgtccacc
    15121 caggactcac ccatcacacc atcgtggcgc tgcaggctgc ccattctggc ccgtgcctct
    15181 tcaccggcgg catttttcac cgttcttgcc gttttgtcgt cactgacaat gcgtccggcg
    15241 aatttggtgc cggcgttctg catcagtgtg gcggtgttgg tggcggaggt ctgcccttca
    15301 attcgctcct gatcctgcgc cgtcattatc agggaaaact ccagtgaacg tacctgggtg
    15361 gcctccacag caatgcggtc tgtgtagtag gcaccgactt cgtcaaggat acagatatag
    15421 gggaagctgc ctttgtattt tttgacttcc agggtctgtg catctgtgcc ttccagccgg
    15481 tagcccaggt cacgggcgag gagcatgctc tgctgggtga cgaacatacg accgagtgcc
    15541 gacgtggtat gagccgatgt gtccagtgca gggatcatca caatcagaat gcggtcgctg
    15601 tgaatactgt cgcgaatatc gatgtcgccg gcatctgccg caaagacgtc cccgaaggtt
    15661 tcggcaaagg ttgtgaacgt ttccgagaac tgaccggaga gataagcatg ctgtttacgg
    15721 ggctcctcag tccaggctga cggcgttctc accagtgaca tatcgaatcc cgggacatcc
    15781 tgcaggtagt ttcgcagtgg tgcgatagct tcttccggcc actggtcatc aaggccacgg
    15841 caatagagtt ttgccatccc ttccagcgtc atatgttccc gtaacatctg cagggacatg
    15901 gttttacctt cccggacgca ccagaatttt gtgccaaaga ccagggcctt gttcatggct
    15961 atcgcccgtg actgccattc accgccctga acgttctggg gcagcatgga ctgcatggtt
    16021 tcggcggtga aggcttcggt gctgaatgcg aacgggttcc aggtgtttga ctgtgggcgg
    16081 gatttttcgc cgctctgaat gatttcactg cgggattttc cgccgttcat gaagttgatg
    16141 acctcgatat catcctccct gccaaaacgt cgggagagat accagattgt cctcgtggta
    16201 tcgttctgtg cctttccgtc aacaaaggta aagcctcttc cccagcagag cgggttgagc
    16261 agccaggcaa aggtggtttc tgttttaccg ccaccggtgg tggcaaaaaa catgacatgc
    16321 cgggtgaggt catccatgct cagccagagc tcacgaccga tatcattgat acggcggtaa
    16381 ccgacataaa aaatcccccg tgcgggggat gtttcatctg cttcatactg aaacagggtg
    16441 ggccagaagc tgaagaggct tcgcctgacc tttctgtcct gggaggggtc atcaaggctg
    16501 agcgtcatgg gcatacgcag ggggcatcgc cagcgttgca gcgtgaatat cacggaggtg
    16561 accagggtga acagcagaca gacaggaagg gccagtggcc atatcagggc cgtaatgata
    16621 ctgccggcca gacacagacc aatgatgttc aggttatgca gtgcattcca cagcgggttt
    16681 ccccaggccg tgcggtgtat gaggtcagga ttaacgtggt gctggctcat tgtgatactc
    16741 ctcgctatct gttgctttca ggaagaggcg aacggaagtg gcgtctttca ggatgccgga
    16801 cggcaggtgc cagccggtat ctgataacac ccagggggtt ccgccaaaac cgaactggcg
    16861 gaaagcctgg ttattgacat cgatggcagc acgtgcggcg gtcagacact gtccatcctg
    16921 tgcggccggt gcatcgccgg tgggggccat accactgtca gcggaataca gctgatgcca
    16981 tccggtcgcc cgtttttgtg agtccggttc acacagcagc ggagcagtcc gctttgtact
    17041 ggactccccg ccaatcacgg agaccggata aatcaccacg ctgaattccc gggccagttg
    17101 cttaagcgcg ggttccagca gacggcagtt gatgcatgcg gggtcaagga aggcgtacag
    17161 cgtgcgttca tgaccagtgg acaggttaat ggtgaacatc ccccggtctg cgttgcgttt
    17221 aagtactgtg gccagattac ggcgggcctc agcggcctga acgtgttgtt cttccgtcac
    17281 tgccgtcggg gcgggggcag aagaaggttc ctgctgtgtg ggagccgggc gggccgggat
    17341 gctgttaaca tgatgttccg tatgtgggcg tggtgtctct gtgtcggtta ctggttcagc
    17401 tctccattcc tgagatttag ggtaagccag cgtggaaggg ggctctgcct tcatccagtg
    17461 gtaacctgca actgtcgccg gcagagcaat tccggcgata aagccacaca tcagtgtccg
    17521 ccagaagcca tatttccggg agggatgttt tccttttccg gcatattcgg catccaggca
    17581 ggcatcaatc gctgagagca ttctgccgat gtgatgtccg ggacgttctg catcaacggt
    17641 atcaacaagc accgtttcgg ttatttcgcg aaaaggggaa ctgaaggaca ggtgcatcag
    17701 cctgttatta cggttaatca gtgcgcccag ttctgttacc ggcaggatgg caatcacctc
    17761 ccgattaccg tcagggtaca gggcggtcag ggacaggtgt ttattattca gtgttgcagt
    17821 gtagtggatg gtcattgttc tttttccttg tcggcgggat gctgtttttc ggtggtttcg
    17881 gtggtttcgg tggtttcggt ggtttcggtg gtttcaccag tgtcagtgtc ttcgtcgtta
    17941 tcatggcggc ggggtatcac gtcatccgtg agtgaccagc gggtcctgat ctgacgttta
    18001 cgatcgcgat ctggctgagg ttcgtcccat atcaggccca gccccagcat gtcccgtcga
    18061 agcccttcaa tggcctcttc cacacagggt tccgggcagg ggaggccgag acggctggct
    18121 tcattttctg cccgggcaac ggccaccacg ccggcccctt cgatgaagcc ttttgtggtg
    18181 tttgcccggt gaagggcata gaagagcgtc cggtcaacgc ccttgagcca cagccagttc
    18241 ggaggcgtca ggcgcaaatc atgtgcgtaa agccagacca gaccgctgcg aacgtaacgg
    18301 tgctgcctga gccactgttg tgcgccttct gttttgatga cccggataaa ggcgtttctg
    18361 gccagtgaat acaccggcgt gctgaaacgc ccctggtccc gtttactttt gagcctgcag
    18421 gagagattga ggttgttcat cagggctacc gctgctttcc ggtcaccgag gaagaactgc
    18481 aggccaaaga tggcaaagag cgctttttca tgtggggcca tgtctttcca gctggtcagt
    18541 ggttgcccca gttgcgccat gaagcactgg cgtgtggacg caacatcgag ctgcatgttg
    18601 cggataaggt tgttctgctc cacaaagact tcgggggtta gtgcgacacg gcgctcggga
    18661 cgtttttgtc cgtggaagag tctgttgttg tctccatcag ccagaacggg ggcgatcgcc
    18721 ggtgacagag ctgaaaaaat atggggcagt gtgtggatat tcagtggcct gcgggtaaaa
    18781 cggggctggg ccggatggtg aaaccacatc cacagaaagc cgaatgtcat gggaagcaga
    18841 atcaaccaga ggatccccac tgtccgggac ataacatcca gccactggcc gaaagtgatg
    18901 gttgccgcgt tgttagcagt gatggccagc aggttgtaac ggggggcggc aaaatggtga
    18961 aaaggccccc agtcagtcat cccccacatt gtgtgcagga tcaggcaggt ggcaaagacg
    19021 atgtcgggta aaaagagcca gatgagaagc gcaaagagca gacatatggt gccaacgata
    19081 taccatatgt tcatcgggtc ttctgttgtg ggggagtgtt ggtaagacac gaggattcct
    19141 ccatcgataa atagataaat ttcgggatga ggctgagtgg aattaatgcg aacaatcgcc
    19201 ggtaatgacc gggcagggct gaattcagca gggcattttc acaggaacag cagaaggtgg
    19261 tgagagacag gcacaaaaaa gccgactggt gaggccggct tttttgcgat cccagggaga
    19321 gtgggtggct ctggcccggg ggaagtctct tttcaactaa cgttaccaga atatcgttat
    19381 attcacctga tcgcaatctg ttcgttacag tctattgttc cgggagtagc gatatcgcct
    19441 gaaacaagta taatcagcaa gttagccttg tctggagact taccttcgca tcaatccatc
    19501 gggactatgg atctgtgcta ttcaaagacc tggccggaca aacaggtggt gctgcgtctg
    19561 gtgctctggc cggaggaagt ctcttttcag ctaacaatga aagagctgtt gtgacaggca
    19621 atgtaagagt aaacaacagt gatttcctcc attgatggag aa
//
LOCUS       DAZPDU010000075        19530 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZPDU010000075 DAZPDU010000000
VERSION     DAZPDU010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19530
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.076"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..245
                     /locus_tag="ACHURS_002360"
     CDS             <1..245
                     /locus_tag="ACHURS_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HFR1238070.1"
                     /translation="AHADYPGDEYQFGKEIAESELLWPVDRVQKSLNGELQQINGADY
                     FIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK"
     gene            complement(296..1063)
                     /gene="ygbI"
                     /locus_tag="ACHURS_002361"
     CDS             complement(296..1063)
                     /gene="ygbI"
                     /locus_tag="ACHURS_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFR1238071.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            1259..2167
                     /gene="ltnD"
                     /locus_tag="ACHURS_002362"
     CDS             1259..2167
                     /gene="ltnD"
                     /locus_tag="ACHURS_002362"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HFR1238072.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVANAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            2164..3426
                     /gene="otnK"
                     /locus_tag="ACHURS_002363"
     CDS             2164..3426
                     /gene="otnK"
                     /locus_tag="ACHURS_002363"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HFR1238073.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDDLDTPFTVFSPALSVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            3423..4061
                     /locus_tag="ACHURS_002364"
     CDS             3423..4061
                     /locus_tag="ACHURS_002364"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFR1238074.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            4066..4842
                     /locus_tag="ACHURS_002365"
     CDS             4066..4842
                     /locus_tag="ACHURS_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFR1238075.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            4931..6295
                     /locus_tag="ACHURS_002366"
     CDS             4931..6295
                     /locus_tag="ACHURS_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HFR1238076.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKHQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(6389..7381)
                     /gene="rpoS"
                     /locus_tag="ACHURS_002367"
     CDS             complement(6389..7381)
                     /gene="rpoS"
                     /locus_tag="ACHURS_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFR1238077.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(7444..8538)
                     /gene="nlpD"
                     /locus_tag="ACHURS_002368"
     CDS             complement(7444..8538)
                     /gene="nlpD"
                     /locus_tag="ACHURS_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFR1238078.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(8723..9349)
                     /gene="pcm"
                     /locus_tag="ACHURS_002369"
     CDS             complement(8723..9349)
                     /gene="pcm"
                     /locus_tag="ACHURS_002369"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFR1238079.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(9343..10104)
                     /gene="surE"
                     /locus_tag="ACHURS_002370"
     CDS             complement(9343..10104)
                     /gene="surE"
                     /locus_tag="ACHURS_002370"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFR1238080.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(10085..11134)
                     /gene="truD"
                     /locus_tag="ACHURS_002371"
     CDS             complement(10085..11134)
                     /gene="truD"
                     /locus_tag="ACHURS_002371"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFR1238081.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEK
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(11131..11610)
                     /gene="ispF"
                     /locus_tag="ACHURS_002372"
     CDS             complement(11131..11610)
                     /gene="ispF"
                     /locus_tag="ACHURS_002372"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFR1238082.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(11610..12320)
                     /gene="ispD"
                     /locus_tag="ACHURS_002373"
     CDS             complement(11610..12320)
                     /gene="ispD"
                     /locus_tag="ACHURS_002373"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFR1238083.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(12339..12650)
                     /gene="ftsB"
                     /locus_tag="ACHURS_002374"
     CDS             complement(12339..12650)
                     /gene="ftsB"
                     /locus_tag="ACHURS_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFR1238084.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(12844..13167)
                     /locus_tag="ACHURS_002375"
     CDS             complement(12844..13167)
                     /locus_tag="ACHURS_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFR1238085.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(13217..13822)
                     /gene="cysC"
                     /locus_tag="ACHURS_002376"
     CDS             complement(13217..13822)
                     /gene="cysC"
                     /locus_tag="ACHURS_002376"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFR1238086.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(13822..15249)
                     /gene="cysN"
                     /locus_tag="ACHURS_002377"
     CDS             complement(13822..15249)
                     /gene="cysN"
                     /locus_tag="ACHURS_002377"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFR1238087.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(15251..16159)
                     /gene="cysD"
                     /locus_tag="ACHURS_002378"
     CDS             complement(15251..16159)
                     /gene="cysD"
                     /locus_tag="ACHURS_002378"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFR1238088.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            16411..17448
                     /gene="iap"
                     /locus_tag="ACHURS_002379"
     CDS             16411..17448
                     /gene="iap"
                     /locus_tag="ACHURS_002379"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFR1238089.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   17469..17741
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=17591..17619
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaacac"
     gene            complement(17838..18131)
                     /gene="cas2e"
                     /locus_tag="ACHURS_002380"
     CDS             complement(17838..18131)
                     /gene="cas2e"
                     /locus_tag="ACHURS_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFR1238090.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNIESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(18128..19051)
                     /gene="cas1e"
                     /locus_tag="ACHURS_002381"
                     /pseudo
     CDS             complement(18128..19051)
                     /gene="cas1e"
                     /locus_tag="ACHURS_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
     gene            complement(19048..>19530)
                     /gene="cas6e"
                     /locus_tag="ACHURS_002382"
     CDS             complement(19048..>19530)
                     /gene="cas6e"
                     /locus_tag="ACHURS_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFR1238091.1"
                     /translation="RFFVLSQERPAESDIFTIECRPFAPELRTGQSLCFNLRANPTIC
                     KAGKRHDLLMEAKRQVRGQVEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQ
                     QQLRRENSRQLIQFSSVDYTGMLTVTAPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA
                     "
BASE COUNT         4605 a         5270 c         4896 g         4759 t
ORIGIN      
        1 tcgcacatgc agattatccg ggtgatgaat atcagtttgg taaagaaata gcagaaagcg
       61 aattactctg gcctgttgat cgtgtgcaga aatcgcttaa tggcgagtta caacaaataa
      121 acggcgctga ttattttata tttggacata tgatgtttga taacattcag acgttcgcta
      181 accagattta tattgatacc ggcacgccga aaagcgggcg gctgtcattt tataaaataa
      241 agtagtctca tgcttcttct gtgaagcatg agtaaccaaa tgttattgca ggccattatt
      301 catttttcgc taccagcaaa gagagatcct gcttcgccag cgcgcgactg gcactctccg
      361 gcaaaccgtc gtctgtgata atctgatcaa actcgcttaa tggtaacgcc agccatgtcg
      421 ccacctgacc atatttcgtc gcatcacaga ccaaaactcg ctggcggctg gcactggcaa
      481 tcgcccgttt caccgtgact ttatcttccg ctggcgtaga aatcccccgc acactccatg
      541 acgatgcaga aataaaagcc tgatcaatca tcaggctgcg cagcatggtc gcagcggctt
      601 ccccgacaca ggaacggttt tcccgacaca ctgcaccgcc agtgtgaata attgtgcaat
      661 tactgttgtc gagcaagtag ttcgcaataa cgaaatcgtt tgtgaccaca gtcagtgact
      721 ccatgtgaat cagatgctgt gctatcgcta acgtggtcgt tcccgcatcc agatagatac
      781 aacttcccgg ctgaacaaga cttgccgcca gcttgccaat agccgctttt tgcgtcattg
      841 ccagcgcagt ttttacctga tgagaaggtt catgcgccac gcgtcccgga gactggacgc
      901 ctccggacac cagcacaacg gctccctgct gctccagttt ttgtaaatcc cgacgaatgg
      961 tcatatgtga cacattcatt ctgtccgtta gttcagcaat actgacaatg cctttttcag
     1021 ctaccatctc aaggatgatt tggcgacgct ctacgggtat caacttttgc tccttccttt
     1081 gtcctgctga cattctacgc tatttgcctg cgaaacgtgc gcggcgcaac taacgcttag
     1141 ttcacataaa ataacacaca atgttaattt atgtgaatca gctcaccata tcgttatctt
     1201 ccagcgctta tattcacaat atcaaacaaa atatcactta aattaacaat gagagcagat
     1261 gaaaacggga tctgagtttc atgtcggtat cgttggctta gggtcaatgg gaatgggagc
     1321 agcactgtca tgtgtccgcg caggtctttc tacctggggc gcagacctga acagcaatgc
     1381 ctgcgctacg ttgaaagagg caggtgcttg cggggtttct gataacgccg cgacgtttgc
     1441 cgaaaaactg gacgctctgc tggtgctggt ggccaatgcg gcccaggtta aacaggtgct
     1501 gtttggtgaa acaggcgttg cacaacatct gaaacccggt acggcagtaa tggtttcttc
     1561 cactatcgct agtgctgatg cgcaagaaat tgctaccgct ctggctggat tcgatctgga
     1621 aatgctggat gcgccagttt ctggtggtgc agtaaaagcc gctaacggtg aaatgactgt
     1681 catggcctcc ggtagcgata ttgcctttga acgactggcc cccgtgctgg aagccgttgc
     1741 cggaaaagtt tatcgcatag gtgcagaacc gggactaggt tcgaccgtaa aaattattca
     1801 ccagttgtta gcgggcgtac atattgctgc cggagccgaa gcgatggcac ttgcagcccg
     1861 tgcggggatc ccgctggatg tgatgtatga cgtcgtgacc aatgccgccg gaaattcctg
     1921 gatgttcgaa aaccggatgc gtcatgtggt ggatggcgat tacaccccgc attcagccgt
     1981 cgatattttt gttaaggatc ttggtctggt tgccgataca gccaaagccc tgcacttccc
     2041 gctgccattg gcctcaacag cattgaatat gttcaccagc gccagtaacg cgggttacgg
     2101 gaaagaagac gatagcgcag ttatcaagat tttctctggc atcactctac cgggagcgaa
     2161 atcatgatca agattggcgt tatcgccgat gattttaccg gcgcgacgga tatcgccagt
     2221 tttctggtgg aaaacggtct accaacggta caaattaacg gtgttccaac aggtaaaatg
     2281 ccggaagcaa tcgacgcact ggtgatcagc ctgaaaacgc gctcctgtcc ggtggttgaa
     2341 gccacacagc aatcgctggc ggctctgagc tggttgcaac agcaaggttg caaacagatc
     2401 tatttcaaat actgctctac tttcgacagt acggcgaaag gtaatattgg cccggttacc
     2461 gatgccttaa tggatgatct cgacacgccg tttacggtct tctctccggc cctgtcggtc
     2521 aacggacgta cggtttatca ggggtatttg ttcgtaatga atcaactgct ggccgaatcc
     2581 gggatgcgcc atcacccggt aaatcccatg accgacagct atcttccccg tctggttgaa
     2641 gcgcaatcca cagggcgctg cggcgtcgtt tcggcacatg ttttcgaaca aggcgtggat
     2701 gccgttcgtc aagagctggc tcgcttacag caagagggct accgctacgc ggtgcttgat
     2761 gcgctgaccg aacaccatct ggaaattcag ggagaagcct tgcgcgatgc cccactggta
     2821 acgggcggtt ctggtctggc gattggcctg gcccggcagt gggcgcaaga aaacggtaac
     2881 caggctcgcg aagcagggcg tccgctcgct gggcgcggcg tagtgctctc cggttcatgc
     2941 tctcaaatga ccaaccgcca ggtggcacat taccgtcaaa ttgcaccagc ccgtgaagtt
     3001 gatgtggcac gctgcctctc aaccgaaact ctggccgctt atgcacacga actggcagag
     3061 tgggttctgg gccaggaaag tgtacttgct ccactggttt ttgccaccgc cagcactgac
     3121 gcattggcag caattcaaca gcaatacggt gcacaaaaag ccagtcaggc agtagaaact
     3181 ctgttttctc aactagcggc gcggttagca gcggaaggcg tgacacgctt tattgtcgca
     3241 ggcggtgaga cctccggcgt agtcacacag agcctgggca taaaagggtt tcatattggc
     3301 ccaaccattt cccccggcgt gccgtgggta aacgcactgg ataagcctgt ctcactcgcc
     3361 cttaaatctg gcaacttcgg tgatgaagcc tttttttcac gagcccaaag agagttttta
     3421 tcatgagcga tttcgcaaaa gtagagcagt ctttgcgaga ggagatgacg cggattgcca
     3481 gttcattctt tcagcgcggc tatgcaaccg gttcggctgg caatctgtcg ctgcttttac
     3541 ctgacgggaa tttactggcg acaccgacag gttcatgcct gggcaatctc gatccgcagc
     3601 ggctttccaa agtcgccgcg gatggcgaat ggttaagtgg tgacaaaccc tcgaaagagg
     3661 tgctctttca tctggcgctg tatcgcaaca atccgcgctg taaagcggtg gtgcatttgc
     3721 acagcacatg gtcgacggcg ctttcctgcc tgcaagggct ggacagcagc aacgttattc
     3781 gcccgttcac accatacgtg gtgatgcgga tgggaaatgt cccgctggtg ccttattacc
     3841 gaccgggcga taaacgcatc gcacaggatc tggcggaact ggcagcagac aatcaggctt
     3901 ttttactggc aaatcatggc ccagtggttt gcggtgaaag cctgcaagaa gccgccaaca
     3961 atatggaaga gctggaggaa acggcaaagc tgatttttat tctcggtgac cgcccgatcc
     4021 gttatctgac cgcaggtgaa attgcggaat taaggagtta atgcaatgcc tcgttttgca
     4081 gctaatttat ccatgatgtt caccgaagtg ccttttattg aacgcttcgc cgcagcgcga
     4141 aaagccgggt tcgatgctgt ggaatttctg tttccctatg actactccac cctgcaaatc
     4201 caaaagcaac tggagcaaaa ccatctgaca ctggcgctgt tcaataccgc tcccggagat
     4261 attaatgcgg gggaatgggg attatccgcc cttcccggac gtgagcacga agcacacgca
     4321 gacatcgacc tggcactgga atatgcattg gcgctcaatt gtgaacaagt ccatgtgatg
     4381 gcaggcgtcg tgcccgctgg cgaagacgct gaacggtacc gggcagtatt tatcgataat
     4441 ctccgctacg ctgccgaccg ctttgcgcta cacggcaagc gaatattagt tgaagcactc
     4501 agccccgacg ttaagcctca ttatctcttc tccagccagt atcaggcact ggctattgtt
     4561 gaagaggttg cgcgagataa cgtgtttatt caactagata cttttcatgc gcaaaaagta
     4621 gatggcaacc tgacgcattt aatccgcgac tacgctggga aatatgcgca cgtacaaatt
     4681 gccggactac ctgatcgaca tgaaccggac gatggagaaa tcaactatcc gtggctgttc
     4741 cgcctgttcg atgaggtggg atatcagggc tggatcggtt gtgaatataa acctcgtggc
     4801 ctcaccgaag aagggcttgg ctggtttgac gcctggcgct aattcgttaa atccctaatt
     4861 acaacgtacc catacatccc cccatctacg gatggggtag gttaactata actttcagac
     4921 agggtttccc atgtccacaa ttacattgtt atgcattgcg ttagctggcg taatcatgct
     4981 gttgctgctg gtcatcaagg caaaggtaca accattcgtt gctctgctcc tcgtcagcct
     5041 gttagtcgca cttgcggcgg gtataccggc gggcgaagtg ggtaaagtga tgatcgccgg
     5101 gatgggcggt gtgcttggtt ccgtcactat tattattggt ctgggcgcta tgctggggcg
     5161 tatgatcgaa cactctggtg gtgcagagtc actggctaat tatttcagtc gcaagttagg
     5221 tgacaaacga actatcgctg cgctgactct ggcagcgttc ttcctcggta ttcccgtctt
     5281 ctttgatgtc ggctttatta ttcttgcgcc aatcatttac ggttttgcca aggttgccaa
     5341 aatatcgcca ctcaaatttg gcctgcctgt cgctgggatc atgctaactg tacatgtggc
     5401 ggtaccgccg catccaggcc ctgtcgccgc agcggggtta ctccacgcag acatcggctg
     5461 gctaaccatc atcggtattg cgatttctat tcccgtaggg attgttggct actttgcagc
     5521 gaaaataatc aataagcacc aatatgcgat gtcggtggaa gtactggaac agatgcaact
     5581 ggctccggcc agtgaggaag gcgcgacaaa attaagcgat aaaataaatc caccgggcgt
     5641 cgcgctggtc acctcgctaa ttgttattcc tatcgcgatt atcatggcgg gtacggtttc
     5701 cgcaacactg atgccgcctt cgcatcccct gcttggtacg ctacagctga tcggctcacc
     5761 aatggtagct ctaatgattg cgctggtgct ggcattctgg ttattggctt tacgtcgcgg
     5821 ctggagctta caacacacca gcgacattat gggctcagcc cttcccactg cggcggtagt
     5881 gattttggtt actggtgctg gaggggtatt tggcaaagtg ctggtggaat cgggcgtcgg
     5941 caaagccctt gccaacatgc tacaaatgat tgacctgcct ctgttaccag ccgcatttat
     6001 tatttcactg gcgctgcgtg catcgcaggg gtcagcaacc gtagcaatcc tgacaaccgg
     6061 cgggttactc tcagaagcgg tgatgggatt gaatccgatt cagtgcgtat tggtgacgct
     6121 ggcagcctgc tttggtgggc ttggggcgtc acatattaat gactcagggt tctggattgt
     6181 gaccaaatat ctggggttgt cggtagcaga cggtctgaaa acctggacag tgttaacgac
     6241 cattctcggt tttaccggat tcttaattac ctggtgcgta tgggcggtaa tttgacctta
     6301 ataaaaaggt ccgatgggca tcggaccttt tattgtgcac agaaaaggcc agcctcgctt
     6361 gagactggcc tttctgacag atgcttactt actcgcggaa cagcgcttcg atattcagcc
     6421 cctgcgtttg caggatttca cgcaaacggc gcaggccttc aacctgaatc tggcgaacac
     6481 gttcacgggt gaggccaatt tcacgaccta catcttccag tgttgccgct tcgtacccca
     6541 gcaaaccgaa tcgacgtgcc agtacttcac gctgtttggc gttcagctcg aacagccatt
     6601 tgacgatgct ctgcttcata tcgtcatctt gcgtggtatc ttccggaccg ttctcttttt
     6661 catcggccag gatgtccagc aacgcttttt cggaatcacc acccagcggg gtgtctaccg
     6721 aggtaatgcg ctcgttaaga cgaagcatac ggctgacgtc atcaactggc ttatccagtt
     6781 gctctgcgat ctcttccgca cttggttcat ggtccagctt atgggacaac tcacgtgcgg
     6841 ttcgcaggta aacgttcagc tcctttacga tgtgaatcgg caaacgaata gtacgggttt
     6901 ggttcataat cgcccgttca atcgtctggc gaatccacca ggttgcgtat gttgagaagc
     6961 ggaaaccacg ttccgggtca aacttctcta ccgcgcggat cagccccagg ttgccctctt
     7021 cgataaggtc cagcaacgcc agaccacgat tgccataacg gcgggcaatt tttaccacca
     7081 gacgcaagtt actctcgatc atccggcggc gagaggcgac atctccacgc agtgcgcgac
     7141 gcgcaaaata aacttcttct tcggccgtta acagtggtga ataaccaatc tcaccaaggt
     7201 aaagctgagt cgcgtccaac acacgctgtg tggctccctg cgataacagt tcctcttcgg
     7261 ccaaatcgtt atcactgggt tcctcttcta ctaaggcctt ttcgtcaaaa acctcaactc
     7321 cgttctcatc aaattccgca tcttcattta aatcatgaac tttcagcgta ttctgactca
     7381 taaggtggct cctacccgtg atcccttgac ggaacattca agcaaaagcc tggttccgcc
     7441 gatttatcgc tgcggcaaat aacgcagcgg gtttacggat ttccccttgt aacgaatttc
     7501 aaaatgcaag cgtgttgaac tggttccggt gctacccatg gttgctattt tttgccccgc
     7561 cttcacttct tgttgttccc ggaccagcat tgtgtcgtta tgggcgtagg cactcaggta
     7621 atcatcatta tgtttgatga taatcagatt accgtagccg cgcagcgcgt taccggcata
     7681 aacaacgcgg ccatctgcgg tcgcgataat tgcctgtcct ttgctgcctg cgatatcaat
     7741 ccccttgttg cccccctcag aagcgccaaa ggtttcgatc actttgccct cagtcggcca
     7801 gcgccaggtg gagataggcg tactggttga tgtactgctg acagtcggct cggttgtgct
     7861 tgctgttggt accgttacag gcgctgtgac tgtggtcgca gttggcttgt tgttcggcaa
     7921 cattttgtta gcactctgtt cacccgaaga ctcagaatac gtaattgtcg gttgcgacgc
     7981 aacagcaacg gtggaatttt gtgcaggctt gatcacaact ccttgctctg ctgcgtcggc
     8041 ctgggtaatg gcatttccgc cagtgattgg cgtaccggaa gcattaccca cctgcaaggt
     8101 ctgaccaacg ttcagcgcgt atggtgcctg aatattgttg cgctgagcaa ggtcacggaa
     8161 atcgttgcca gtaatccagg cgatatagaa aagtgtgtcg ccttttttca cggtataggt
     8221 actgccgcta taactgcctt tcggaatgtt cccatactga cggttataga cgatgcgtcc
     8281 gttttccatc tgtaccggct gctgagctac tggctgcact ggctggattt gcggttgttg
     8341 agtagcctga atttgtggct gctgtaccgg ctgaatttgc ggttgctggg ctgtagacgt
     8401 cgtccccatt ttcggcggcg gcgtaatcaa cataccagaa ttagtatttg caggcgcatt
     8461 gccattaacg gagctgaccg gtgccggtgg atttgaagtg tcagaacagc ctgccagcca
     8521 tagcgaaacc agtgacaaag cagcaatgcg gcgaacggtg aattttgggc ttcccgcgct
     8581 catttatccc ccaggaaaaa ttggttaata accagtgaca taattaccgt gcaaggcacc
     8641 ctactgaaca ctggaaaaga tgttcacgat acgctgacct gcggcaaaat aaccaggaaa
     8701 aatccaggta tttcctcacg ttttaagcca gctcaccctt cactaaaggg acaaagcgca
     8761 cggcctccac ggtatcgata ataaattcgc ctccccgacg acgcacccgt ttcaaatact
     8821 ggtgctcctc ccctacgggt aagacgagaa tcccgccttc gtccagctgc gtcattagcg
     8881 cagttggaat ttccggcggt gccgccgtaa caatgatagc gtcaaacggc gcacgtgcct
     8941 gccaaccttg ccatccatcg ccatgacggg ttgaaacatt atgtaaatca agatttttca
     9001 ggcggcgacg tgcctgccac tgcaagcctt taatccgttc aaccgagcaa acatgctgga
     9061 caagatgcgc taggattgcc gtttgatatc ccgaaccagt gccaatttcc agcacccgcg
     9121 actgcggcgt cagctcgagt aattcggtca ttcgcgccac catatacggc tgcgaaattg
     9181 tctgcccctg acctatcggc aaggcgatat tgtcccaggc tttttgttca aacgcttcat
     9241 caacgaattt ttcacgcggc acggcggcaa gtgcattcag cacctgctca tcctgaatac
     9301 cttgcgcacg taattgatcc agaagtgctt gtacgcgtct gcttaccatt gcgtgccaac
     9361 tcccacgctg tttaaccagt ctgaaaccac atcttgcgcg ctatgcgcag ttaaatccac
     9421 atgcagcggc gtgatggaga catagccctc atctaccgca gcaaaatcgg tccccggacc
     9481 ggcatcacat ttaccgcccg gcgggccaat ccagtacagc gtattgccgc gcggatcttg
     9541 ctgcgggatc acctgatctg ccggatgtcg tgtaccgcag cgcgtcacgc gaataccttt
     9601 gatttgatcc aagggtaaat ccggaacgtt aatattaaga atacgtccgg tgcgcagcgg
     9661 ctctttacac agtgcgcgca aaattgaaca ggttaccgcc gcggcagtgt cgtaatgttt
     9721 atgcccgtca agcgagacgg ccagcgccgg aaaacctaaa tgacggcctt ccatcgcggc
     9781 ggctaccgta ccggaataaa taacgtcatc ccccagattc ggcccggcgt taatgccgga
     9841 cacaacaatg tccgggcgcg gacgcatcag agcattcacg ccaagataga cgcaatcggt
     9901 cggggttccc atttgcacag caatatcacc attttcaaag gtaaacgtgc gcagggagga
     9961 ttccagtgtc agagaatttg aagcgccgct gcggttacga tcgggggcga ccacctgaac
    10021 gtcagcaaac tcacgcaagg ctttcgccag cgtttgtata ccgggtgcat gtaccccgtc
    10081 atcattactc agcaatatgc gcataatcac ctgttgtgtt gataagttcc ctgacaacgc
    10141 tggttgcaaa actacccgcc ggaagccaga aacggatctc tacggtgacg tcatcccacc
    10201 aattccagct taattgttgc ggatacagca acatcgctct gcgcgcggct tcaacttttt
    10261 cgcgcaccag taaagcttgt aattcagttt ctgcggcgac agctgccttt tcgaatgcca
    10321 gcgcttcacg ctgagttccc cattcgccac tacccggcaa tgcggcggtt atcatcagct
    10381 ctttatcgtt gacgcgacgc tgtaattccg ccagttcttc ggtggttgcg acaaaccagc
    10441 taccacgtcc ggctaattgt agcgcatcgc cgtcaacaac ttgattaacg tctgcttttt
    10501 tgaggcgctc agcaacaatc tgattaaaca acgcactgcg ggctgccgac aaccaaaaac
    10561 tccgtttatt gcgatcgcgc accggagtat tggtttgcgc ccagcgctgc gccccctgca
    10621 agttgctacc gccaatccca aaacgttggg caccgaagta gttcggtaca ccttttacgc
    10681 aaatatcgat cagacgttgt tcaacgtcat cgcgattgct cacttcgcgc agaaccaggg
    10741 taaaggcgtt acctttcagc gcgcctaaac gcagcttgcg cttgtgccgc gcatactcca
    10801 gcacctggca gccttccagt tgaaaggcgc tcagatcggg catttccttg cctggcacgc
    10861 gagcgcataa ccactgttcc gtaacagcat gtttgtcttt ttgcccagcg aagctgactt
    10921 cacgggcatg aattttcagg aatttcgcca gtgcatccgc cacaaaacgg gtattgcagc
    10981 cgtttttgag gattctaacc agaatatgct caccttcacc atcaggctca aagcccaaat
    11041 cttccaccac cacaaagtct tccggattgg ctttcagcag cccggtgcct tgcggtttac
    11101 cgtggaggta agtgagatta tcaaactcaa tcattttgtt gccttaatga gtagcgccac
    11161 cgcttcacag gcaatccctt ccccacgtcc ggtaaatcca agtttttccg tagtagtggc
    11221 tttcacgtta acatcatcca tatggcagcc gagatcttcg gcaataaaca cgcgcatttg
    11281 tggaatgtgc ggcaacatct tcggtgcctg agcgataata gtgacatcga cgttgcccag
    11341 ggtataaccc ttcgcctgaa tacgacgcca ggcttcgcgt agcagctcgc ggctgtcggc
    11401 acctttaaat gccggatcgg tatccgggaa cagcttgccg atatccccca gcgccgccgc
    11461 gccaagcaat gcatcggtca acgcatggag cgccacgtcg ccatcagaat gcgccagcaa
    11521 tcctttttcg taaggaatgc gtacgccacc aatgataatt gggccttcac cgccaaaggc
    11581 atgtacgtca aaaccgtgtc caattcgcat tatgtattct cctgatggat ggttcgggtg
    11641 aggtaaaact cggccagtgc caaatcttcc gggcgcgtga ctttaatgtt atccgcacgg
    11701 ccttcgacca actgaggatg gaatccgcaa tattccagcg ccgaggcttc gtcggtaata
    11761 gtcgcgcctt catttagagc gcgcgtcaga cagtcatgta acagctcacg agggaaaaat
    11821 tgcggcgtca gcgcgtgcca taagccgttg cgatcaacgg tatgagcaat ggcatttttg
    11881 cccggttcgg cacgtttcat cgtatcgcgc actggtgcgg ctaggatccc tcccgtgcgg
    11941 ctggtttcgc tcaacgccaa caatcgcgcg aggtcatcct gatgcagaca aggacgagcg
    12001 gcgtcatgca ccaataccca ctgcgcgtcg ccagcggctt tcaaacctgc cagcacggaa
    12061 tcggcacgct catcaccgcc atctacaacg gtgatttgcg gatgattcgc cagaggaagt
    12121 tgtgcaaaac ggctatcgcc aggacttatg gcaatgacga cacgtttcac ccggggatgc
    12181 gccagcagcg catgcaccga gtgttcaaga atggtttgat taccgattga gagatattgc
    12241 ttaggacatt ccgtttgcat tcgacggcca aatccggccg ccggaaccac ggcgcaaaca
    12301 tccaaatgag tggttgccat gttaattccc gggctgattt atcgattgtt ttgccccgca
    12361 gactgtgcgc gcttcgacgc gtcaggcacc agacgataaa aagtttcgcc cggcctggtc
    12421 atgctgagtt cattacgcgc acgctcttcg agcgcctcct ggccgccatt gagatcgtca
    12481 atttcggcaa aaagttgatc gtttcgcgct ttaagtttcg cgtttgtagc ttgctgtgcc
    12541 gccacatcat cattgacgcg ggtatagtca tgtataccgt tcttaccgaa ccacagcgaa
    12601 tactgtagcc agaccagaat agccagcaac agcagcgtta gtttacccat cctgccccct
    12661 gaaaaacggc atcatcatcc catgcatccg aagacgactc tacatcctct gttggggata
    12721 ccgcgacaac gcgggcaaat gtaccacatt tgtccattgt tacgtatacc cagggcgtgc
    12781 agaacataat ctcattatta gttacggttt gaattatgaa cagaggagac aagaaagtac
    12841 aaattagccc agtagccaca taaacagtgc gccaaacata atgcctactg tcaccagggt
    12901 gaaaacaata ctgtagcgta gctttccgtc catcaatgaa tgcagcgcaa tccccaccac
    12961 taccgcgacg ggcatcagcg ccagaaagaa aggccaggtg tagataaaga agaacagcgt
    13021 gttagagcca taaatcaaca tcggcatcgc cagcgcaaat aaccaggaga taaaaccgac
    13081 cacggcacca ggcagtgacc atgtggtttc ttcatcctca gtaaggctgt cgttatttgt
    13141 tagtgtaatg ttatggctat tacgcatatt tgatcctgtt actttgacga accgggcatg
    13201 gaaacccggt ggtgtctcag gatctgataa tatcgttctg tctcaacaga tctaataatt
    13261 gctgtaccaa atttgttact aattgttcac cattgagatg aatttctgcc gattcaggcg
    13321 cttcgtaaac ggaatctatt cccgtaaagt tgcgcagttc accggcacgc gctttcttat
    13381 ataagccttt tggatcgcgg gcttcgcaaa tcgccagcgg cgtatcgaca aacacttcga
    13441 taaagcgccc ttctcctacg cgttcacgaa ccatctggcg ttcggcgcgg tgtggcgaga
    13501 taaatgcggt cagcaccacc agtccggctt caaccatcaa attcgccact tcaccgacgc
    13561 gacggatatt ctctttacga tcggcatcgc taaaaccgag atcgctgcat aatccgtggc
    13621 gaacattatc gccatccagc agatacgtac tgacgccgag tttatgtaac gcctcctcca
    13681 gcgccccggc gaccgttgat ttaccggacc cggagaggcc ggtaaaccac agcactacac
    13741 cacgatgacc gtggtgtagc tcgcgttgtt gcacagtgac cggatggcta tgccagacga
    13801 cgttttcgtc atgcagcgcc attatttatc ccccagcaaa tcgcgcgcgc cccagtgcgg
    13861 aaagtggcga cgaaccagag cattcaattc cagttcgaat gcactgaatt cagatggcgc
    13921 agcagttgcc tggctaactg gctcgtgcac cataccggca cccacggtca cattgctcag
    13981 gcgatcgata aaaatcagcc cacccgtcac cggattttgt tgataacgat ctaacaccag
    14041 cggctcgtca aaagtgagat ccacgaggcc gatcccattc agtggcaggt tttcaacttc
    14101 acgctgggta aggttattaa tatcaacctg atagcgaatg ccatcaacac gcgcgcgcgt
    14161 cttcttaccg gcaattttga tgtcgtaact ctgccctgga gaaagcggct gttccgccat
    14221 ccataccaca tccaccgacg cgctctgcac cgccggtaac gcttcgtctg ccgccagcag
    14281 cagatcgcca cggctgatgt cgatctcatc cgtcagcacc agggtgatcg cttctccggc
    14341 aaaggcttct tcgcgatcac catcaaaagt cacgatccgc gcgacgtttg attccacacc
    14401 agagggcagc acttttacac gttgcccgac ttccacgcga ccggatgcca gcgttccggc
    14461 gtagccacga aaatcgagat tcgggcgatt aacgtactgc accgggaagc gcattggctg
    14521 agcatccacc actcgctgga tctccacggt ttccagcact tcgagcagtg tcggaccgct
    14581 gtaccacggc atactttcac tttgcgatgc cacgttgtcg ccttccagtg cagagagcgg
    14641 cacaaagcgg atatccagat tacccggcag ctgcccggca aaggtcagat aatcttcacg
    14701 aatacgggtg aacgtctctt cactgtaatc caccagatcc attttgttga tcgccacgac
    14761 cagatgtttg atccccaaca gtgtggagat aaaactgtga cgacgggttt gatcgagcac
    14821 gcctttacgg gcatcgatca gtaagatcgc cagttcacat gtcgatgcgc cagtcgccat
    14881 attgcgagtg tactgctcgt gccctggggt gtcggcgata ataaatttac gcttctcggt
    14941 agagaaatag cggtaggcca catcaatagt gatgccctgt tcgcgctcag cttgcaggcc
    15001 gtccaccagc agagccagat ccagcttttc gccctgggta ccgtgacgct tactgtcgtt
    15061 atgcagcgat gagagctgat cttcatagat ttggcgagta tcgtgcagca ggcgaccaat
    15121 cagagtgctt ttgccgtcat cgacgctgcc acaggtcaga aaacgcagca ggcttttatg
    15181 ttgttgcgca atcatccagg cttcgacgcc gccttcattg gcgatttgtt gtgcaagtgc
    15241 ggtgttcatc ttaaaaatac ccctgacgtt ttttcagctc catagatccc gcctggtcgc
    15301 ggtcaatcac gcggccctga cgttcactgg tggtggaaac cagcatctct tcgatgattt
    15361 ccggcagtgt ttgtgcattt gactccaccg caccggtcag cggccagcag cccagcgtac
    15421 ggaaacgcac catccgtttt ttaatcactt cgcccggttg caggtcgata cggttgtcat
    15481 caatcatcat caacataccg tcgcgttcca gaaccggacg ttccgcagcg agatacagcg
    15541 gcacaatgtc gatattttcc agccagatgt attgccagat atcctgctcg gtccagttag
    15601 agagtgggaa gacgcggatg ctttcgcctt tgttaatttg cccgttgtag ttgtgccaca
    15661 gctccgggcg ctgatttttc ggatcccagc gatggaagcg gtcacggaaa gagtagattc
    15721 gctctttagc gcgggatttc tcttcgtcac ggcgcgcacc accgaaggcg gcatcaaaac
    15781 cgtatttgtt cagcgcctgt ttcaggcctt cagttttcat aatatcggta tgtttcgcgc
    15841 tgccgtgcac gaatggatta atccccatcg ccacgccttc cgggttttta tgcaccagca
    15901 gctcgcagcc gtaggctttc gccgtacgat cgcggaactc atacatctcg cggaatttcc
    15961 agccggtatc gacatgcagc aacgggaaag gcagcgtacc tggataaaac gccttgcgcg
    16021 ccaaatgcag catgacgctg gaatctttac cgatagagta gagcatcacc ggatttgaga
    16081 attctgccgc cacctcgcga ataatgtgga tgctttccgc ctccagttgc cgcaggtgag
    16141 taagtcgtat ttgatccata accgttcctt tgcaataccg ctattttctt gccatcagat
    16201 gtttcgacta tagggagcgt aagagaacga atgaaattac caattagaat gagtagttcc
    16261 ttaacggaat aacgatttgg caaagctaat atcaaaaagt gcttaaggca ccggatttct
    16321 ggcgtttagg aagatttgaa attgttttag cgcagcggca gtttcatact atggcggtaa
    16381 aaaaatttgc atggtattta aggactcact atgttttccg cattgcgcca ccgtaccgct
    16441 gccctggcgc tcggcgtatg ctttattctc cccgtacacg cctcgtcacc taaacctggc
    16501 gattttgcta atactcaggc acgacatatt gctactttct ttccgggacg catgaccgga
    16561 actcctgcag aaatgttatc tgccgattat attcgccaac agtttcagca aatgggttat
    16621 cgcagtgata ttcggacatt taatagtcgg tatatttata ctgcccgcga taatcgtaag
    16681 agctggcata acgtgacggg aagtacggtg attgccgctc atgaaggcaa agcgccgcag
    16741 cagatcatca ttatggcgca tctggatact tacgccccgc tgagcgatgc tgacgccgat
    16801 gccaatctcg gcgggctgac gttacaagga atggatgata acgccgcagg tttaggtgtc
    16861 atgctggaac tggcagaacg cctgaaaaat acgcctaccg agtatggtat tcgatttgtg
    16921 gcgaccagcg gcgaagagga aggaaaatta ggcgctgaga atttactcaa gcggatgagt
    16981 gacaccgaaa agaaaaatac gctgctggtg attaatctcg ataacttaat tgttggcgat
    17041 aaattgtatt tcaacagcgg tgtaaaaacc cctgaagcag taaggaaatt aacgcgcgac
    17101 agggcgctgg caattgcgcg cagtcacgga atagccgcaa cgaccaatcc gggtttgaat
    17161 aaaaattatc cgaaaggcac tgggtgttgt aatgacgcag aaatattcga caaagcgggc
    17221 attgctgtac tttcggtgga agcgactaac tggaatcttg ggaataagga tggttatcag
    17281 caacgcgcaa aaacagccgc attccctgcg ggaaatagct ggcatgacgt aagactggat
    17341 aatcaacagc atattgataa ggcacttcct ggaagaatag aacgtcgctg ccgtgacgtt
    17401 atgcggataa tgctacctct ggtgaaggag ttggcgaagg cgtcttgatg ggtttgaaaa
    17461 tgggagctgg gtgttctacc gcaggggcgg ggaattctaa gtgatatcca tcatcgcatc
    17521 cagtgcgccc ggtttatccc cgctggcgcg gggaactctt actgcttggt atgcggaatc
    17581 acaccctgaa cggtttatcc ccgctggcgc ggggaacacc ctgacgcgcc gcagtattta
    17641 tctgctctgg ccggtttatc cccgctggcg cggggaacac gggtgcgtgt ggctgccagt
    17701 gccggagaac ggcggtttat ccccgctggc gcggggaaca cactaagcat acataactgt
    17761 ttttaaacaa atttattccg catcaacaat ctaccaacta aattaaaaca tttccttatt
    17821 tttaaagaac ggataactta ttgattatca acaggaagaa aagaaaccaa acgtaaccca
    17881 tccaaatcca ccggaatacg tctgttttct ccccaggtct gaaattcaaa acccgactcg
    17941 atattggtcg cccaggccat caccacattt ccgcaaccag ccagttgggt aatttgctgc
    18001 cagatcatct cccgaatacg ttttgatgta tcaccaacat acacaccggc acgcacttcc
    18061 agtagccaga ttgcgagccg tccacgtaag cgcggaggga cattttctgt aacaaccacg
    18121 accatgctca tccgccgcgc ccccggtgac cactatcacc cagcgtttca ggttcaggga
    18181 tggcaggcgg taacatatcc ggcgcgggtt gtggtggttc aatttcacct gcagcaagga
    18241 cttcctcaat taacggtatt aatttgcccg ttaacttagt gctacggaaa atatcgcgac
    18301 aggctaatct gacttcttta tcaggttctg cgggttgcct cgctgctatt tcaaatgcct
    18361 ttggcacaac cgaatcaaat ttaatgatat cggctatgtc ataaacaaat gaaagcggtt
    18421 tgccactatg gataaaccca atagcgggcg catatcccgc ggctaatact gccgcttcag
    18481 atataccgta cagacatgat gtagcagcac tgatgcagcg attcacaaca tcgccttttt
    18541 cccagtcttt aggatcgtat ttgcgaccat tccatttcac accatattgt ttcgccagta
    18601 atgcatacgt ctggcgaacg cgagatccct caattccacg taactgctcc actgaacggc
    18661 gagctggcgg tggttcacga aaacgtaatt catacatttt gcgcaccacc ttcaaccgta
    18721 gatcttccgt taaagccagc tttgcctagt agagtaattt atctgcccgc gcccctccgg
    18781 gttgtccgga ggagtaaacg cgaacgcccg cttcaccgac ccagaccagc agtgttccca
    18841 ccgtggcggc cagatggact gccgcgtggg aaactcttgt tcccggttcg agcataatgc
    18901 aggcgaccga tcccactgga atatgcgtgc ggatcccggt tttgtcgatc agcacgaaag
    18961 cgccgtccag tacgtcgatt tgaccgtact ggaggaagat catggaagtg cgatctttta
    19021 atgggatcgg gctcagcggt acaaacgtca cgcctctgct ccgggtttga tcagcatcag
    19081 accacaaccg aacgcccggc ttttgccata cccctgactg agacgttgca gaaataaccc
    19141 aggagcggtg accgtaagca tccccgtata atccacgcta ctgaactgga tcagttgccg
    19201 ggagttttcc cgccgcagtt gctgttgtcg gtaggcatca accgaagtat ccagcagtgt
    19261 aaaaccgctt ctctccccct gcgctgccag ccagtccagc gcagcctgtt gttgatgcaa
    19321 ccagacatca cttccttcca cctgccccct cacctgccgt ttcgcctcca tcagcaagtc
    19381 gtggcgtttg cccgctttac aaattgttgg attcgcccgc aggttgaaac aaagggattg
    19441 tccggtacgc aactctggcg caaatggtcg gcattcgatg gtgaaaatat cactttccgc
    19501 cgggcgttcc tgcgacagga caaaaaagcg
//
LOCUS       DAZPDU010000076        19372 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZPDU010000076 DAZPDU010000000
VERSION     DAZPDU010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19372
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.077"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(31..1860)
                     /gene="glmS"
                     /locus_tag="ACHURS_002383"
     CDS             complement(31..1860)
                     /gene="glmS"
                     /locus_tag="ACHURS_002383"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFR1238092.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLDGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2022..3392)
                     /gene="glmU"
                     /locus_tag="ACHURS_002384"
     CDS             complement(2022..3392)
                     /gene="glmU"
                     /locus_tag="ACHURS_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFR1238093.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNVQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKVGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3743..4162)
                     /locus_tag="ACHURS_002385"
     CDS             complement(3743..4162)
                     /locus_tag="ACHURS_002385"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFR1238094.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4183..5565)
                     /gene="atpD"
                     /locus_tag="ACHURS_002386"
     CDS             complement(4183..5565)
                     /gene="atpD"
                     /locus_tag="ACHURS_002386"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFR1238095.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5592..6455)
                     /gene="atpG"
                     /locus_tag="ACHURS_002387"
     CDS             complement(5592..6455)
                     /gene="atpG"
                     /locus_tag="ACHURS_002387"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFR1238096.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6506..8047)
                     /gene="atpA"
                     /locus_tag="ACHURS_002388"
     CDS             complement(6506..8047)
                     /gene="atpA"
                     /locus_tag="ACHURS_002388"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFR1238097.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8060..8593)
                     /gene="atpH"
                     /locus_tag="ACHURS_002389"
     CDS             complement(8060..8593)
                     /gene="atpH"
                     /locus_tag="ACHURS_002389"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFR1238098.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8608..9078)
                     /gene="atpF"
                     /locus_tag="ACHURS_002390"
     CDS             complement(8608..9078)
                     /gene="atpF"
                     /locus_tag="ACHURS_002390"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFR1238099.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9140..9379)
                     /gene="atpE"
                     /locus_tag="ACHURS_002391"
     CDS             complement(9140..9379)
                     /gene="atpE"
                     /locus_tag="ACHURS_002391"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFR1238100.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9426..10241)
                     /gene="atpB"
                     /locus_tag="ACHURS_002392"
     CDS             complement(9426..10241)
                     /gene="atpB"
                     /locus_tag="ACHURS_002392"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFR1238101.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10250..10630)
                     /gene="atpI"
                     /locus_tag="ACHURS_002393"
     CDS             complement(10250..10630)
                     /gene="atpI"
                     /locus_tag="ACHURS_002393"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFR1238102.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAYTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(11247..11870)
                     /gene="rsmG"
                     /locus_tag="ACHURS_002394"
     CDS             complement(11247..11870)
                     /gene="rsmG"
                     /locus_tag="ACHURS_002394"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFR1238103.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(11934..13823)
                     /gene="mnmG"
                     /locus_tag="ACHURS_002395"
     CDS             complement(11934..13823)
                     /gene="mnmG"
                     /locus_tag="ACHURS_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFR1238104.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLVGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(14202..14645)
                     /gene="mioC"
                     /locus_tag="ACHURS_002396"
     CDS             complement(14202..14645)
                     /gene="mioC"
                     /locus_tag="ACHURS_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFR1238105.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(14735..15193)
                     /gene="asnC"
                     /locus_tag="ACHURS_002397"
     CDS             complement(14735..15193)
                     /gene="asnC"
                     /locus_tag="ACHURS_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFR1238106.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLAEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15345..16337
                     /gene="asnA"
                     /locus_tag="ACHURS_002398"
     CDS             15345..16337
                     /gene="asnA"
                     /locus_tag="ACHURS_002398"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFR1238107.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRENVPSLL"
     gene            complement(16342..17793)
                     /gene="viaA"
                     /locus_tag="ACHURS_002399"
     CDS             complement(16342..17793)
                     /gene="viaA"
                     /locus_tag="ACHURS_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFR1238108.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(17787..19283)
                     /gene="ravA"
                     /locus_tag="ACHURS_002400"
     CDS             complement(17787..19283)
                     /gene="ravA"
                     /locus_tag="ACHURS_002400"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFR1238109.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIHQAQQC"
BASE COUNT         4632 a         5124 c         4848 g         4768 t
ORIGIN      
        1 tcggcagccc ttttttatct atgatagtga ttactcaacc gtaaccgatt ttgccaggtt
       61 acgcggctgg tcaacgtcgg tgcctttgat cagcgcgaca tggtaagcca gcagctgcag
      121 cggaacggtg tagaagatcg gtgcaatcac ctcttccaca tgcggcatct cgatgatgtg
      181 catgttatcg ctacttacaa aacccgcatc ctgatcggcg aagacataca attggccgcc
      241 acgcgcgcga acttcttcaa tgttggattt cagtttttcc agcaattcgt tgttcggtgc
      301 aacgacgata accggcatat cggcatcaat aagcgccagc ggaccgtgtt tcagttcacc
      361 agcagcgtag gcttcagcgt gaatgtagga gatctctttc agcttcaatg cgccttccag
      421 tgcgattggg tactgatcgc cacggcccag gaacagtgcg tgatgtttgt cagagaaatc
      481 ttctgccaga gcttcaatgc gtttgtcctg agatagcatc tgctcaatac ggctcggcag
      541 cgcctgcaga ccatgcacaa tgtcatgttc aatggaggca tccagacctt tcaggcgaga
      601 cagcttcgcc accagcatca acagcacagt taactgagtg gtgaatgctt tagtggacgc
      661 cacgccgatt tccgtacccg cgttggtcat cagcgccaga tcggattcgc gcaccagaga
      721 agaacccgga acgttacaga ttgccagcga accaaggtaa cccagctctt tcgacagacg
      781 caggcccgcc agggtatccg cggtttcgcc agactgtgac aaggtgatca tcaggctgtt
      841 acgacgcacg gcagatttac gatagcggaa ttcagaggcg atttcgacgt cgcacggaat
      901 acctgccagc gattcaaacc agtagcggga aaccataccg gagttataag aagtaccaca
      961 ggcgaggatc tgaatatgct caaccttcga cagcagttcg tcggcgttcg gtcccagctc
     1021 gcttaaatca acctgaccgt ggctgatgcg cccggtaagg gtgtttttta tcgcgttcgg
     1081 ctgttcgtag atctctttct gcatgtagtg acggtaaata cctttatcgc ccgcgtcata
     1141 ttgcagattg gattcgatat cctgacgttt tacttccgcg ccagttttat cgaagatgtt
     1201 taccgaacgg cgagtgattt ccgcaatatc gccctcttca aggaagataa agcgacgggt
     1261 caccggcaac agcgccagct ggtcagaagc gataaagttt tcgcccatac ccaagccaat
     1321 caccagcgga ctaccagaac gtgccgccag cagggtatcc gggtgacggg agtccatgat
     1381 cactgtaccg tacgcaccgc gcagctgcgg gatagcacgc agaacagcct cacgcagagt
     1441 cccgccttgt ttcagctccc agttcaccag atgggcaatc acttcggtgt cggtttcaga
     1501 aacgaaggta tagccacgcg cttttaactc ttcacgcagc ggttcatggt tttcgatgat
     1561 gccgttatgc accaccacaa tgtgttcaga aacatgcgga tgcgcgttag cttctgaagg
     1621 ttcaccgtgg gtcgcccagc gagtgtgagc aataccggtg ccgccatcca gaggatgttc
     1681 ttccgctgcc tgagccagca tctggacttt accgaggcga cgcaggcggg tcatatgacc
     1741 ttccgcatca acaacggcca gaccggcaga gtcatatccg cggtattcca gacgacgtaa
     1801 accttcaaga aggatttctg ctacatcacg ttgcgcgatc gcgccaacaa ttccacacat
     1861 agtttttgat tccgatttat atcgttgtcg gtcaacctgt atgcccgttt tttcgggcgc
     1921 cccgagcctt gtagagagtg gggttatttt tatagttact gcttgtggga gggagattat
     1981 gttatctcct catcccagat gaccggatta cccggccaga attacttttt ctttaccgga
     2041 cgacgccagc cttctttctg agtctgcggc acacggctga tagctaatgc attttcgccg
     2101 acattacgcg tcacagttgt acccgcagca atggtcgcgc ctttgcctac tgttaccggg
     2161 gccaccagct gagtgtcgga accgacaaac acatcgtcgc cgataatggt cttaaattta
     2221 ttcgcaccat cgtagttgca ggtaatggtt cccgcgccga tgttaacgtt atcgccaatt
     2281 tccgcatcgc ccaggtaagt cagatgcccg actttcgagc ctttacccag acgcgctttt
     2341 ttcatctcaa cgaagttacc aacgtgtgca ccttccagca actcagcacc aggacgcaaa
     2401 cgagcaaacg ggccaatagt acaggccgct gccagattcg cgtcttccac aacggtatac
     2461 ggactgattt cgcaatcatc gccaatcacg ctgtttttaa tcacgcaacc ggtgccgatt
     2521 ttcacgcgat gaccgagagt cacgttgccc tcgatgataa cgttagtatc aatttcaaca
     2581 tcgcgcccgt gagtaagcgt accgcgcaga tcaaaacgcg ccggatcgcg cagcataacg
     2641 cctgctaaca gcagtttttc agcctgttcg gactggtaaa cgcgctccag acgggagagt
     2701 tgcaggcggt tattcacacc ttctacttcg cttaaacgtt gcggatgaac ggcgacgatt
     2761 tcacgccctt cctgatacgc cagcgcaata atgtcggtga tgtagtattc gccctgaaca
     2821 ttattgttgg tcagcttcgc cagccagcgt ttcatatctg cgccgttagc gatcagaatg
     2881 ccggtgttga tctcctgaat ctgacgctgc tcgtcggtgg catctttgtg ctcaacaatg
     2941 ccggtaactt tgccattttc acgggtgata cgtccataac cggtcggatc atccagtttc
     3001 accgtcagca gaccaatgcc accctgcggt ttagcatcac gcaggcgctg gagtgtttcg
     3061 acagagatca gcggcacgtc gccgtagagc attaaaatgt cttcatcatc ggcaaagaaa
     3121 ggtgcggcct gctgcattgc atgacccgta cccagctgct ctgcctgaag cacccagtta
     3181 aggttgtcgt ctttcagcgc ctgtttaagc agatcgccgc cgtgaccgta caccaagtga
     3241 acgtgcgctg cgcctaattc attcgcagca tcaatgacat gctgaaccat cgctttcccg
     3301 gcaagggtat gcagcacttt cggaagatcg gaatacatgc gcgtgccttt gcctgcggca
     3361 aggatcacta cgctcatagc attattcaac atacgcgtcc tgactgtaat ttgagaacga
     3421 atttaaaccg cttcaccttg aaaaaactac atttttttca tcgtgaaatg gacagaggat
     3481 aaattgttca ataacggatt atccccgctg acgaaagtgc cattttcgac catcgtcacg
     3541 ccttttgtct gcagaaaatt aaggcagtac cttcatttaa gcgtcaggtg gatgtttttg
     3601 ctcttatttc gatcaatgaa taaacgggaa aacaggggtg ttttatatca gcaggatcta
     3661 tgtgaacgct cttcaggacg gatcacgcgt aaaaaaaagc cagcctgttt ccagactggc
     3721 ttttgtgctt ttcaagccgg tgttacatcg cttttttggt caactcgata acgcgcagct
     3781 gcgcgatcgc tttggccagt tccgcagacg cctgagcgta atctacgtcg ccgtgagagc
     3841 tgctaatgtg ctcttcagcc ttacgtttcg cttccatggc tcgcgcttcg tcgagatcct
     3901 ggccgcgaat tgcggtgtcg gccagaacgg tcacgttgcc aggctgcact tcaagaatgc
     3961 cgccagacag atagataaac tcttcgtgac cgtgctgttt cacgatgcga atcataccag
     4021 gcttaatggc ggtgagcagc ggtgcgtggc cagggtagat ccccagttca ccttcgctac
     4081 ccgttacctg gattttctcg accagaccag agaacatttg ttgctctgcg ctgacgacgt
     4141 ccaggtggta agtcattgcc atatcaccct ccgattaagg cgttaaagtt ttttggcttt
     4201 ttccacagct tcttcgatgg aaccgaccat gtagaacgcc tgctccggca ggtgatcgta
     4261 ttcgccttcc atgatgcctt taaagccacg gatggtgtct ttcagggaga cgtatttacc
     4321 cggagaaccg gtgaatactt ctgccacgaa gaacggctgg gacaggaagc gctggatctt
     4381 acgagcacgc gctaccacca gtttgtcttc ttcagacagt tcatccatac ccaagatggc
     4441 gatgatgtct ttcagttcct gataacgttg caggatggac tgaacgccac gcgcggtgtc
     4501 gtagtgttcc tgaccaacca ccagcgggtc cagctgacgg ctggtggagt ccagcgggtc
     4561 aacggccggg tagataccca gagacgcgat ctgacggctc agtaccacgg ttgcgtcaag
     4621 gtgcgcaaag gtggttgccg gagacgggtc agtcaagtca tccgcaggta cgtataccgc
     4681 ctgtacggag gtgatagaac cagttttggt ggaggtgata cgttcctgca gaacgcccat
     4741 ctcttccgcc agggtcggct gataacctac cgctgaaggc atacggccca gcagtgcgga
     4801 tacttccgta ccggccaggg tgtaacgata gatgttgtca acgaacagca gaacgtcacg
     4861 accttcgtca cggaatttct cagccatggt cagaccggtc agagcaacgc gcagacggtt
     4921 tcccggcggc tcgttcatct ggccatacac cagggatact ttgtcgataa cgttggagtc
     4981 ggtcatttcg tggtagaagt cgttaccctc acgagtacgt tcacctacgc ccgcaaacac
     5041 agagtaaccg gagtgctcga tcgcgatgtt acgaatgagc tccatcatgt ttacggtttt
     5101 acctacaccc gcaccaccga acagaccaac tttaccgccc ttagcgaacg gacacatcag
     5161 gtcgataact ttgataccgg tttccagcag ttcctgagag tttgacagct cttcgtagga
     5221 aggtgctgcg cggtgaatcg cccaacgctc ttcttcaccg atctcgcctt tcatgtcgac
     5281 cggttcaccc agtacgttca tgatacggcc cagagtcgct ttacctaccg ggacttcgat
     5341 cgggtgttcg aggtctttta catccagacc gcgacgcaga ccgtcggagg aacccattgc
     5401 gatggtacgc acgataccgc cgccgagctg ctgctgaact tccagcacca gacgctcatt
     5461 accattttgc acctcaagag catcgtacac gcgcggtacg gcatcctgag ggaattcgac
     5521 gtcaactacg gcgccgatta cctggacaat ctttccagta gccatcttaa atcctctacg
     5581 aaataacctg tttaaaccgc ggcggccccc gagacgatct cggtgagttc ctgagtaatg
     5641 ctggcctgac gagctttgtt gtataccaac tgcagctctt taatcaggct gccgccattg
     5701 tcggtcgcgg ctttcatcgc caccatacgg gcggcctgct cgctggccag gttttcaacc
     5761 acgccctgat aaacctgaga ttcgacataa cgacgcagca gggtatccag caacgccttc
     5821 ggatcgggtt cgtacaggta atcccaggat ttatgtttca gatcatcatc atctgatgcc
     5881 ggtaacggca gcagctggct gatggtcgga acctgagaca tggtgttaat aaatttgttg
     5941 ctgacaatgt aaagcttgtc cagacggcct tcgtcgtaag cctgcaacat cacttttacc
     6001 ggaccgatca gttcggacag ggaagggtta tcccccatgc cggtgacctg ggcaacaaca
     6061 ttgccgccca cggagttgaa gaacgacacg cctttcgagc cgatcattgc gaggtcgcat
     6121 tgaacgcctt tgtcggtcca ggtcttcatt tccgccagta gttttttgaa caggttaatg
     6181 ttcaaaccac cgcacaaacc acggtcggtc gacaccacca ggtagcccac gcgtttaacg
     6241 tcgcggtctt ccaggtaagg gtgcttatat tccagattac cgtgtgcaag gtgaccaatc
     6301 actttgcgca tggtttctgc ataaggacgg ctggccgcca tgcgatcctg cgatttacgc
     6361 attttggaag cggcgaccat ctccatcgct ttagtgatct tttgcgtgtt ctggacgctt
     6421 gcgatcttac tacgtatctc ttttgcgccg gccatgagct tctcctcaat gccttgcggc
     6481 ctgccctaag gcaagccgcc agacgttacc aggattgggt tgctttgaag gaatcgagga
     6541 tgcctttcag cttgccttcg atttcgtcgt tgtagccacc ggtctggttg atctcttgca
     6601 tcaacggagc gtgatcacgg tcgacgtaag ccagcagagc ggcttcgaag ctgccaattt
     6661 tcgacagttc aacatccgcc aggtaaccac gttctgctgc gaacagaacc agagactgct
     6721 gcgcaacgga catcggcgca tactgtttct gtttcagcag ttcggtcact ttctgaccgt
     6781 ggtcaagctg cttacgtgtt gcatcgtcaa ggtcggatgc aaactgagag aacgctgcca
     6841 gttcacgata ctgtgccaga gcggtacgga taccaccgga cagttttttc atgatcttgg
     6901 tctgtgctgc accaccaaca cgggatacgg aaatacccgg gttaaccgca ggacgaatac
     6961 cggcgttgaa caggttggtt tccaggaaga tctgaccatc ggtaatggag attacgttgg
     7021 tcggaacgaa cgcagaaacg tcacccgcct gagtttcgat aatcggcagt gcggtcagag
     7081 aaccggtttt ccctttcact tcacctttgg tgaaggcttc aacgtattcg gcgttaacac
     7141 gtgcagcacg ctccagcaga cgagagtgga ggtagaaaac gtcgcccggg aatgcttcac
     7201 gtcctggcgg acgacggagc agcagggaga tctgacggta agcaacagcc tgtttagaca
     7261 ggtcatcgta aatgatcagc gcatcttcac cgcggtcacg gaagtattcg cccattgcgc
     7321 aaccggcata cggtgccagg tattgcagtg cagcggattc agacgcggtt gctaccacaa
     7381 cgatggtgtt agccagtgcg ccgtgctctt ccagtttacg taccacgtta gaaatggtgg
     7441 acgctttctg gccgatagcg acatagatac atttgatacc ggaatcgcgc tggttgatga
     7501 tggcatcgat agccagtgcg gttttacctg tctgacggtc accgatgatc aattcacgct
     7561 gaccacgacc gattgggatc atggagtcaa cggctttata accggtctgt accggctgat
     7621 ctacggactg acgttcgata acgcccggag cgattgcttc tacagcagag aagccgtcgt
     7681 gatccagcgg acctttaccg tcgattggtg cacccagagt gttaaccaca cggcccagca
     7741 ggccacggcc aaccggaact tccaggatac ggccagtaca cttaactttc atgccttcgg
     7801 caaggtcagc gtacggaccc ataacaaccg cacctacaga gtcgcgctca aggttcagtg
     7861 cgatagcgta acggttaccc ggcagggaga tcatttcacc ctgcatacaa tcggccaggc
     7921 cgtgaatgcg gataacaccg tcacttacag aaacaatagt accttcgttg tgagcttcac
     7981 tcacaacatt gaactgagca atgcgctgct tgatcagttc gctgatttcg gtggaattca
     8041 gttgcatgct ccagtcccct taagactgca agacgtctgc aaggcgctca agacgaccgc
     8101 gtacgctgcc atcaatgacc atatcacccg ctcggatgat aacgcctgcc attacagact
     8161 tatcgatttt gcaattcagc ttaactttgc gtgacagacg tttttccatc gcagcagaaa
     8221 ttttcgcgag ctgttgttca ctcagtgcgg cagcggaaat gacgtctacc tcagcggtag
     8281 cctcactcac ggcacgaagg tgaataaact gctccagaac atccgggagc gcgttaagac
     8341 gaccattttc agccattacc cgaatcaggt tctgaccgtt ttcgtccagt tgctcaccac
     8401 aaactgcgat aaacgactcg gcgagcgttt ctggcgcaag cgcgccagag agaagctctg
     8461 ccatttgttc gtttttggtt acctcggcgg caaacgccag catgtcctgc cagcgttcta
     8521 cactttggtg ttcgacggca aagtcaaaag ctgctttggc gtaggggcga gctaccgtaa
     8581 taaattcaga catcagcccc tccctcctta cagttcagcg acaagtttat ccacgatgtc
     8641 gctgttagca gcttcatcca cggaacgttc gatgatcttc tcggcgccag caacagccag
     8701 gatagcaact tgcttacgca gctcttcacg ggcacgttta cgctcggctt caatttctgc
     8761 ctgcgcctgg gccacgattt tagtacgttc ctgttctgcc tcagctttcg cttcgtccag
     8821 aatctgcgag cggcgtttgt tcgcctgctc gatgattacc tgggcttccg ctttcgcttt
     8881 tttcagctgg tcggtcgcgc tggcctttgc aaggtcaagg tccttatgtg ctcgttctgc
     8941 ggaagcaagg ccgtcagcaa tttctttttg acgtttttcg atggctgcca ttaatggcgg
     9001 ccatacgtac ttcatgcaga acagaacgaa caggacaaac gcgatggcct ggccgaggat
     9061 tgttgcgtta agattcacag cacaatgcct ctatttagtt aacgttctga tattgctctt
     9121 taaataaaag caacgcttac tacgcgacag cgaacatcac gtacagaccc agacctacag
     9181 cgatcatcgg gatagcatcc accagaccca taacgataaa gaactgagta cgcagcagag
     9241 gaatcagatc aggttgacgc gctgcgcctt ccaggaattt acccccgagg atgccgatac
     9301 cgatcgcagc accgattgcc gccagaccca tcatcacagc ggcagccatg tacagcagat
     9361 ccatattcag gttttccatg acagtctcca gtttgtttca gttaaaacgt agtagtgttg
     9421 gtaaattaat gttcttcaga cgccatcgac agatagacga tcgtcagaac catgaagatg
     9481 aaggcttgca gcgtaatgat caggatgtgg aaaatggccc acggcacatt caggatccac
     9541 tgtgaccacc acggcaacag accagcaatc agaatgaaaa tcagctcacc ggcatacatg
     9601 ttaccgaaca gtcgcaaacc gagtgaaact ggtttggaca gcaggcttac cccttcaagg
     9661 attaagttga caggaatgaa cgcccagtga ttgaacggct gcagcgtcaa ctctttcgtg
     9721 aagccgccga tgcctttcat tttgatgctg tagaacagaa tcaggataaa tacgcccagt
     9781 gccatagaca gcgttacgtt cacgtccgca gacggaacca cacgcagtgc aggcagaccc
     9841 agtacatgtt cagcaatgta cggcagcagg tcgataggca gtaaatccat caggttcatc
     9901 aggaataccc agacgaagat cgtcagggcc agcggagcaa tcagcttgct tttgccatgg
     9961 tacatgtctt tcacgctacc attaacaaag ccgatcacca gctcaatcgc ggtctgaaac
    10021 ttacctggca caccgctggt cgcctttttg gctacgctac ggaataaaac caggaacaac
    10081 agacccagca ccaccgagaa gaacatggag tcaatattga ttgtccagaa ggtggctggg
    10141 gggttttgtg gatccaccag cgagaatgta cgcaggtcca gctgaaggtt attcaggtgg
    10201 tgtcctatgt aatcctgcgg cgtcatattt tctgaagcca tgatgccttt taccctttgt
    10261 tgttaattac agccggtgcc agtatctgaa ccaccagcac caaaacccac gtaacgatca
    10321 gcggcaagaa taccgccttt aaaaccgcca acgccaccac cagtaacacc aacatcgcca
    10381 gaactttgaa agcttcgcca aatgcgaatg tccaggccac ccggcctttc gctggtgtat
    10441 acgcctggtg acgccaggca aatatcataa acaaaacgtt aggcagaaag actgccaggc
    10501 ccccgcttat tgcagagacg ccccagaagg ggtctttgag gctgaacagc aatccacttg
    10561 ctatcaccac cagtaactga acgagcagaa gcttccgagc aacgtttcga ctcacgagcg
    10621 acacagacat cacgtttttc actcctgctc ccttcgaggt atgccgcgtg tcgtataaaa
    10681 ctttctttaa ggcttagagt caagcatcaa aaagcggtca aattatacgg tgcgcccccg
    10741 tgatttcaaa caataagtag ccaaaaggtg aataaatgtt taaatatttt tccagtgcat
    10801 acaattgcga cttttccgct aaccctgttc gatcatgaaa aactgtaaat aacgcgtaaa
    10861 cactagtgat aaagcgtgct tcagatcaca tattgcgcat gttcgcgcac agtatattta
    10921 tttacttggc aaatgatgcc tttgcaagtt tatgatattt cagtctaaaa acagatactg
    10981 ttttaataaa tgacatttac acaacaaaaa ccacccattg acatttttaa taatgtttta
    11041 acagccaatg atggttctta gcgccgattt ttagcagact gatattttca ctaatgactt
    11101 attttctgct taccaaaaaa agccacgtta tcttgttgat acaaaagagt gaacgtggcg
    11161 ttaaatgtaa ccagttatat cagtagaaaa cttggttgtt gttaacagtc taaccggtca
    11221 attttttatg atttttttga taaaaattaa attttatttg ctttaatcac caccagatga
    11281 cgttcgccat ccagagctgg aacctgaagt ttaaccactg attcgacctg atattcttcg
    11341 ggcaacaaag cgatttcatc ttccggcatt tgccctttca gcgcgtagaa acggccttgc
    11401 tcaccaggaa gatggtggca ccagctcacc atatcgttca gagaggcaaa agcgcggcta
    11461 attacgccat caaatggcgg ctctgaagga aactcttcta ccctgctctg tactggctca
    11521 atattctcca gtttaagctc atgttgcacc tgacgaagga aacgcacgcg tttaccaagg
    11581 ctatccaaca gagtgaaatg ggcttcagga cgcacgatag agagtggaat gcctggcagt
    11641 cctggtccgg tgccgacatc gataaaccgt tcaccttgca gatacggtgc caccacaatg
    11701 ctatcgagaa tatggcgtac cagcatctca ttaggatcgc ggaccgaagt caggttgtac
    11761 gctttgttcc atttatgcag catattcacg taggcaataa gctggttttt ctggtgatcg
    11821 gtaagcgaaa tacctgcgtc tttcagcagt aaggagagtt tgttgagcac ggtgattacc
    11881 tgttcttgat gcgttgcctg gtaagcgggt gcttaccagg catttttaat gcgttatgcg
    11941 ctacgacgca gcataccctg ttttttcagc cacaccagca gaatggagat agccgcaggc
    12001 gtgacgccag aaatacgcga agcctggccg atagaggccg gtttgtgatc gttaagtttg
    12061 gcgatcactt cgttagaaag accggatacc tggcggtaat ccagtgtcgc gggtagcagg
    12121 gtgttctcgt tacgcagctg cttttcgatc tcatcttgct ggcgcgcgat ataaccttcg
    12181 tatttaacct gaatctcaac ctgttccgcc gcctgttcgt ctgtcaacgc aggggcaaac
    12241 ggcgtcagcg tggttaattt ttcataagtc atttccggac gacgcagcag atcttcacca
    12301 ctggcttcac gggaaagcgg cgcagtcagg tgagcattca cttcggctgc agcttccgcc
    12361 gacggggtta cccaggtcga tttcagacgc tgacgctcac gctcgatatt ctcaagtttc
    12421 tcgttaaagc gcgcccaacg ttcgtcatcc accaggccca gttcacgacc gatttcagtc
    12481 aaacgcagat ccgcattatc ttcgcgcagc atcagacgat attctgcgcg cgaagtaaac
    12541 atacgatacg gttctttggt tcctaaagtg cacaggtcat caaccagtac gccgagatac
    12601 gcctgagaac gcgccggagc ccaaccttct ttgtcagcag acagacgggc agcgttaaga
    12661 ccgaccagca aaccttgcgc agcggcttct tcgtaaccgg tagtgccgtt aatctgacca
    12721 gcaaagaaca gcccctggat aaacttgctc tccagcgtcg gtttcaggtc gcgtggatcg
    12781 aagaagtcat actcaatggc ataacccgga cgcacgatct tcgcgttttc catcccctgc
    12841 atggagcgga cgatttgcat ctgcacatcg aacggcaggc tggtggagat accgttcgga
    12901 taaatttcgt tagaggtcag cccttccggt tcaaggaaga tctgatgctg atttctgtcg
    12961 gcgaagcgca tgactttgtc ttcgatcgat gggcagtagc gtgggccgac accttcgatc
    13021 acccctgcgt acattgggct acgatcgagg ttactgcgga tcacatcatg ggttttctcg
    13081 ttggtatgag tgatataaca cggcacctgc tggggatgct gggacgcatt gcccataaac
    13141 gagaataccg gcattgggtt atcgccatgc tgttgcgcca gcacactaaa gtcgatagtt
    13201 cgagcatcaa tacgcggtgg tgtcccggtt ttcagacgac caacgcgcag cggcagttca
    13261 cgcaaacggc gagaaagcgg aatggacggc ggatcaccag cacgaccacc gctgtaatta
    13321 tccagaccga tatgaatttt accgtcgagg aacgtcccaa cggtgagcac gacggctttg
    13381 gcacggaact tcagtcccat ttgggtaaca gcaccgacca cgcgatcgtt ttcgacaata
    13441 agatcttcaa ccgcctgctg gaagatcatc aggttcggtt ggttctccag cgccgtacgt
    13501 accgcctgac ggtagagcac acgatccgcc tgagctcggg tagcgcgaac cgccggtcct
    13561 ttgcttgcgt ttagtatcct aaactggata cccgcttgat cgatcgcttt cgccatcaga
    13621 ccgccgagtg catccacttc ttttaccaga tgtcccttcc caataccgcc gatcgccggg
    13681 ttgcagctca tctgccccag agtgtcgata ttgtgtgtca aaagcagagt ctgttgaccc
    13741 atacgcgccg cggccatcgc ggcctcggtg cctgcatgac ccccgccaat gatgatgacg
    13801 tcaaaaggat ccggataaaa catggtgatt gcctcgcata acgcggtatg aaaatggatt
    13861 gaagcccggg ccgtggattc tactcaactt tgtcggcttg agaaagacct gggatcctgg
    13921 gtattaaaaa gaagatctat ttatttagag atctgttcta ttgtgatctc ttattaggat
    13981 cgcactaccc tgtggataac aaggatccgg cttttaagat caacaacctg gaaaggatca
    14041 ttaactgtga atgatcggtg atcctggacc gtataagctg ggatcagaat gaggggttat
    14101 acacaactca aaaactgaac aaccgttgtt ccttggataa ctaccggttg atccaagctt
    14161 cctgacagag ttatccacag tagatcgcac gatctgtata cttatttgag taaattaatc
    14221 cacgatccca gccattcttc tgccggatct tccggaatgt cgtgatcaag aatgttgatc
    14281 ttcagtgttt cgcctgtctg ttttgcaccg gaatttttga gttctgcctc gagtttatcg
    14341 atagccccac aaaaggtgtc atattcacga ctaccaatac cgattgcgcc aaagcggacc
    14401 gcagaaagat cgggcttctg ttcctgcaat gcttcataga aaggagaaag gttgtccgga
    14461 atatctccgg caccgtgggt ggagctgata atcagccaga tccccgaggc agataaatct
    14521 tccaacagcg gaccgtgcag cgtttcggtg gtaaaacccg cctcttccag cttttcagcc
    14581 aggtgttctg ctacatattc ggcaccgccg agggtgctgc cgctgataag agtgatatct
    14641 gccataaacc gccaccttta ttaagagtgg cgtattgtac gctgtgaacg cgttgggatc
    14701 tacctgtgga aaagtatggg attaaaaaag ccgattatgg cttgatggta cgcatgatcg
    14761 ggttttgcag gacgatcaat gtctcggtgg actgaatttc atcaattgtt tggatcttgt
    14821 tgataagtac atgctggaga gcgtcgatcg aacggcacat tacttttata aagatgctgt
    14881 agtggcctgt tgtgtaatag gcttcagtga cttcagcaag gctttccagc tttgccagcg
    14941 cggaagggta gtctttggcg ctctttaata taatgccgat aaagcagcct acgtcataac
    15001 cgagctgctt cgggctgaca tcaatacgcg ccccggtaat gatccccgcc tgcttcattt
    15061 tctctactcg aacgtgaatc gtccccggac tgacgccaaa ttgtttcgcc agttcggcgt
    15121 aagcggtgcg cgcattgccc attaatgctt ccaggatgcc acggtccaga ttgtcgatca
    15181 gataattttc cataggattt tcttatgcag attgatgatt cattctattt tagccttctt
    15241 ttttaatgaa tcaaaagtgt gttaggcttt ttattgaatg attattgcat gtttgtcggt
    15301 ttttgttgct taatcataag caacaggacg caggagtata aaaaatgaaa accgcttaca
    15361 ttgccaaaca acgccaaatt agcttcgtga aatctcactt ttctcgccaa ctggaagaac
    15421 gtctggggct gatcgaagtc caggcgccga ttcttagccg tgtgggggat ggcacgcagg
    15481 ataacttgtc gggctgtgaa aaagcggtgc aggtaaaagt gaaagctctg cctgatgccc
    15541 agttcgaagt ggttcattca ctggcgaagt ggaaacgtca gaccttaggg caacacgact
    15601 tcagcgcggg cgaagggctg tacacgcaca tgaaagccct tcgccccgat gaagaccgtc
    15661 tttctccgtt gcactcggtc tatgttgacc agtgggactg ggaacgcgta atgggcgacg
    15721 gtgagcgtca attctcgact ctgaaaagca cggtagaggc gatctgggcg ggaattaaag
    15781 caaccgaagc tgcggttagc gaagagtttg gcctggcacc gttcctgccg gatcagatcc
    15841 acttcgtaca cagccaggag ttactgtctc gttatccgga tctcgatgcc aaagggcgtg
    15901 agcgggcgat agcgaaagat cttggcgcgg tattccttgt cgggattggc ggcaagctga
    15961 gcgatggtca tcgccacgac gtgcgcgcac cggattatga tgactggagc accccgtcag
    16021 agctgggtca tgcgggtctg aacggcgata ttctggtgtg gaaccctgta ctggaagatg
    16081 cgtttgagct ttcttccatg gggatccgcg tggatgccga cacgttgaag catcagttgg
    16141 cgctgaccgg tgatgaagat cgtctgcagc tggaatggca tcaggcgctg ctgcgcggtg
    16201 aaatgccgca gaccatcggc ggcggtatcg gccagtctcg tttgaccatg ctgctgctgc
    16261 aactgccgca tatcggccag gttcagtgtg gtgtatggcc agctgctgtt cgcgagaacg
    16321 tcccttctct gctgtaataa tttatcgccg ccagcgtctg agcaggcggc ttcgcatccc
    16381 ggtatcaaag cgccagatat gatcgaaaat gcgcatgatg ccgggtttgc cgtgtgccga
    16441 catcgccacg gcatgaaagc gatgctgatg tacccgctgc agctctttca ctttactcgt
    16501 cacgtcgtca ggcaaccgct gagcgataaa atcagaaatc accaccgcat cggcatcaaa
    16561 ccattccctg ctttgcaagc gttccataat ggcgcgaaaa caactggcaa gatcggtgcc
    16621 gccacgaaac tgctggctta aaaaacggat tgcttgttcg atgccttggg ggcctgaaag
    16681 ctcataacgg acgatctcgg tggaaaatag cataatatag cagcgccggt tttcagcgag
    16741 agcaatgcgc atcaaggcca ggcagaacgc tttcgcacac tgttcattaa agccgcccat
    16801 tgagccggaa gtatccacac agacaataaa cggcccgcgc ggctgttcat cgtaatcttt
    16861 atgtaccacc ggacgttcga tcactttttc acgccacgac tcaccgtgca ggcgataggt
    16921 gagcaactgt ttttccacca gccgacggta aaactcatac tccagttccg ttatccctag
    16981 tgtcgccagt tctggcggca gaagacgtaa aatatcatcg ctttgttgca gaccatcaac
    17041 ctgctcagga accgtcgccg gttcgcgcac catggtgcgg aaggtttcca tctgcgcatc
    17101 gttgcgcggt attgatttgg cttcccgaga acgccccagc tgctctgcca ggcgtttcag
    17161 ttccggctgt tcgttaagaa attcaccgta tttcacaatc aactgatagt cgccacgttt
    17221 aagctgaccg gcgctcatat cccacagacg accagctgca gtattgttat ctgcgagaat
    17281 cggttcaagt tgtccgctca gcgtcatgcg ttcttgaact tcactcaaca gttgttcgcg
    17341 ttcttcttct aatagctgtt gatttaacgt cgttgcttgc acgatcagac ttaaacgcca
    17401 acgctggaga aaaagcgtgt gtaacgctga agttattgtg ctgttagcat caaccaactg
    17461 ccgggcttgt tctgcccatg gggaattcag acgatgcagt aagtccagga tctgtggtag
    17521 ctgcacaata aactgcggcg tggagaggag ctggctttgc tgatagcaca tcacctcttc
    17581 ggtgagttcc ggcgggactc gggcatcttt cagccgactg cgcagcgcct cacgccagcg
    17641 gggaacatca tcagtgattg ccgccttcag gcgtgggaat ttttcaaaga agaccgccag
    17701 ctgcggtgag gccagcagtg cgatgatcat ctcttcgatc aatccctctt cgctgacggc
    17761 cagcatcaca ttaagcgtat ccagcgttag cattgttgtg cctggtgaat ctgtgcaccg
    17821 acatcctgta ggctggcttc aatgcgacct aaccagtcgc cagggataaa cagacatttt
    17881 tgctgttcgc tgaataaagc gtgttgcttg cgccagtcgc tttcaagctc ttccagttgt
    17941 tgttttattt caccaggcag accttcagcc gatgaaccgg gaagtgccag cgtactgcct
    18001 tgtaaactta catcgcgaac gacaagatgt tgggcgctat caacttccag attcagtttc
    18061 tgggcaaagc cgataccgtt cagtttaccg cgaatttcac cacctttgct cagccactgt
    18121 tccagcgcgc tacgctcaaa ggagatatga accacttcca tatcatgcag ttttaacggt
    18181 ttttgcagca gtagagtcag agtggaagca gtaacgttaa caggaagttg atactgctga
    18241 cgacggctga aaatgccgcc cagacgaatt accgttaagg ctgttttatc gctttgttgc
    18301 tgctggagtt gcaggtgacg ttgcacaatc gcgccaaggc gagtcaacat cccttgctgt
    18361 tgccaggcat gaccggtcat caatacatca atttgttgtt gtatcaaatt caggctttgc
    18421 gcgtcatacc acaggcaatc tttcagcaaa atgagatcta ccggggcaac agcactgcga
    18481 ccgctaaaaa aggcgctggc ctgcaataat cggatcgctt ttttccagcg acgatccgag
    18541 acataaggcg catccggtaa tttatccagt tgctggcgca gcataaaaat cagctcaaat
    18601 acatgatcgg gcagcgtaat ttcaccaatc tctttctgcc agcgttcata ttcttcatct
    18661 gtgacctgca aggcatcagg aaccggattg tcgttttcat cctgttgact ggtcagcatg
    18721 gagcggaaat tcgctttatc ctgcacttta tctaaccaca gacgaatcag catgcggtca
    18781 tataacgctt ccagactgct gtctgcttcc ggcagctcgt tggaggccgc caccagcaga
    18841 cgcatcggga ttttttctac gtgtgcaccg ttgcggaact ggcgctcgtt aatggcggtg
    18901 agcaaggtat taagaattgc cgggcccgct ttccagatct catccagaaa gacaatttct
    18961 gcttccggca ggtaaccgct ggttaaacgt tcatagcgcc cttcatcttt tagcgcctga
    19021 atagaaaggg gaccaaaaac ttcttccggc gtggagaagc gggtcatcag atattcaaac
    19081 gcgcgggcat tctgaaaggc gaattttaag cgccgggcga tcaaactttt ggcaatacct
    19141 ggcgggccaa ggaggaacac actttcacca cttaatgccg ctaataaaca caagcggatg
    19201 gcgtggctac gttcataaag ccccttttcc aacgaactgc tcaggcggga aattctttcc
    19261 gctaataaat gagggtgagc cataatgaag tggcgtcctt tcgtcaaaag ttctgcgtaa
    19321 attgcgagta tagacgtttc ttgctggtgg ctaaaatagt ctcaaagggg gg
//
LOCUS       DAZPDU010000077        19182 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZPDU010000077 DAZPDU010000000
VERSION     DAZPDU010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19182
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.078"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(230..1879)
                     /gene="pgi"
                     /locus_tag="ACHURS_002401"
     CDS             complement(230..1879)
                     /gene="pgi"
                     /locus_tag="ACHURS_002401"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFR1238110.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     regulatory      2116..2312
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2404..3753
                     /gene="lysC"
                     /locus_tag="ACHURS_002402"
     CDS             2404..3753
                     /gene="lysC"
                     /locus_tag="ACHURS_002402"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFR1238111.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            4005..4277
                     /locus_tag="ACHURS_002403"
     CDS             4005..4277
                     /locus_tag="ACHURS_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFR1238112.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(4410..5282)
                     /gene="rluF"
                     /locus_tag="ACHURS_002404"
     CDS             complement(4410..5282)
                     /gene="rluF"
                     /locus_tag="ACHURS_002404"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFR1238113.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            5630..6467
                     /locus_tag="ACHURS_002405"
                     /pseudo
     CDS             5630..6467
                     /locus_tag="ACHURS_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313031.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
     gene            6537..7343
                     /locus_tag="ACHURS_002406"
     CDS             6537..7343
                     /locus_tag="ACHURS_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR1238114.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEVHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            7353..7760
                     /locus_tag="ACHURS_002407"
     CDS             7353..7760
                     /locus_tag="ACHURS_002407"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFR1238115.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            7760..8254
                     /locus_tag="ACHURS_002408"
                     /pseudo
     CDS             7760..8254
                     /locus_tag="ACHURS_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            8320..9117
                     /locus_tag="ACHURS_002409"
     CDS             8320..9117
                     /locus_tag="ACHURS_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFR1238116.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            9128..9952
                     /locus_tag="ACHURS_002410"
     CDS             9128..9952
                     /locus_tag="ACHURS_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFR1238117.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VIMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            10004..11232
                     /gene="sorE"
                     /locus_tag="ACHURS_002411"
                     /pseudo
     CDS             10004..11232
                     /gene="sorE"
                     /locus_tag="ACHURS_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
     gene            11615..12304
                     /gene="pepE"
                     /locus_tag="ACHURS_002412"
     CDS             11615..12304
                     /gene="pepE"
                     /locus_tag="ACHURS_002412"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFR1238118.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(12395..14026)
                     /locus_tag="ACHURS_002413"
     CDS             complement(12395..14026)
                     /locus_tag="ACHURS_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFR1238119.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(14246..17929)
                     /gene="metH"
                     /locus_tag="ACHURS_002414"
     CDS             complement(14246..17929)
                     /gene="metH"
                     /locus_tag="ACHURS_002414"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFR1238120.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFETLGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAEAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWEVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNEPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            18129..18953
                     /gene="iclR"
                     /locus_tag="ACHURS_002415"
     CDS             18129..18953
                     /gene="iclR"
                     /locus_tag="ACHURS_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFR1238121.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         4523 a         5015 c         5021 g         4623 t
ORIGIN      
        1 acgattaaaa atctatttta ggattaacct cacggtatga tttccgttaa atcacagaca
       61 aggggattta tctgataaaa caatgccgac ttattgtcgg cattgttttt aaggttattt
      121 aactcctcat actatgcatt aatgtcgata ttggcatcag gctccggttg ccggatgcgg
      181 cgtaaacgcc ttatccggcc tacatatcgg catcaggcac gcatgatgat taaccgcgcc
      241 acgctttata gcggttaatc agaccattgg tcgagctatc gtggctgctg atttctttgt
      301 catctttcag ctctggcaga atacggttcg ccagttgttt acccaactcc acgccccact
      361 ggtcgaaggt aaagatgttc aggatcacgc cctgagtaaa gattttgtgc tcatacagcg
      421 caatcaacgc acccaggctg aacggagtga tttcgcgcag caggatggag ttggtcgggc
      481 ggttaccttc gaacactttg aacggcacca cgtagtcaag cgttgccgga tctttaccct
      541 gatcgcgata ttcctgctca accacttcgc gggatttacc aaacgccagc gcttcggtct
      601 gggcgaagaa gttagacagc agtttctggt ggtgatcaga gagcgggtta tgggtgatag
      661 ccggggcgat gaaatcgcac ggtaccattt tggttccctg gtggatcagc tggtagaacg
      721 cgtgctgacc gttagtgcct ggttcacccc agataatcgg gccagtctgg taatccacaa
      781 cgttaccgtt acggtcaaca tacttaccgt tggactccat attgccctgc tggaagtacg
      841 ccgcgaaacg gtgcatatac tggtcatacg gcagaatcgc ttcagtttcc gcaccaaaga
      901 aattgttgta ccagatgcca atcagcgcca gcaggacagg caggtttttc tcggcaggcg
      961 tggtggagaa atgcttgtcc atcgcgtgtg cgccagaaag cagttcaacg aagttatcaa
     1021 agccgatgga gagaacaatc gacaggccaa tcgctgacca caaagagtaa cggccgccga
     1081 cccagtccca gaactcgaac atgttggcag tatcaatacc aaactcgcca acggctttgg
     1141 cattggtgga aagcgccgca aagtgttttg caacgtgttt ttcatcacct gccgctttca
     1201 ggaaccagtc acgcgcgcta tgggcgttgg tcatagtttc ctgagtggtg aaggttttag
     1261 atgctaccag gaacagcgtg gtttccgggt ttactttttt cagcacttca gcgatgtgag
     1321 tcccatcgac gttagaaaca aagtgcatgt tcaggtgatt tttgtacgga cgcagtgctt
     1381 cggtcaccat gtacgggccg aggtcagaac caccgatccc gatgttcact acgtcagtga
     1441 ttgctttgcc ggtataaccc ttccactcac cggagataat cgcttccgag aaggttttca
     1501 tcttctccag caccgcgttg acttccggca ttacgtcttt gccatcaacc agaatcgggg
     1561 tattgctacg gttacgcagc gctacgtgca gcacggcgcg gttttcagtg cggttgatct
     1621 tctcgccaga gaacatcgac ttaatcgcgc ccgccagatc gcactctttc gccagatcct
     1681 gtaatttcgc cagcgtctct tcagtgatgc ggtttttgga gtaatccacc agcatctgat
     1741 cgtcgaatgt tgcggagaac ttagaaaaac gatcgccgtc tttagcaaaa agatcggcga
     1801 tcgtaacgtc tttcatttca tcgaagtgtt tctgtagtgc ctgccaggca gcggtctgcg
     1861 ttggattgat gtttttcatt agcaatactc ttctgatttt gagaattgtg actttggaag
     1921 attgtagcgc cagtcacaga aaaatgtgat ggttttagtg ccgttagcgt aatgttgagt
     1981 gtaaaccctt agcgcagtga agcatttatt agctgaacta ctgaccgcca ggagtggatg
     2041 aaaaatccgc atgaccccgt cgttgacaac cgccccgctc accctttatt tataaatgta
     2101 ctacctgcgc tagcgcaggc cagaagaggc gcgttgccca agtaacggtg ttggaggagc
     2161 cagtcctgtg ataacacctg agggggtgca tcgccgaggt gattgaacgg ctggccacgt
     2221 tcatcatcgg ctacaggggc tgaatcccct gggttgtcac cagaagcgtt cgcagtcggg
     2281 cgtttcgcaa gtggtggagc acttctgggt gaaaatagta gcgaagtatc gctctgcgcc
     2341 cacccgtctt ccgctcttcc cttgtgccaa ggctgaaaat ggatcccctg acacgaggta
     2401 gttatgtctg aaattgttgt ctccaaattt ggcggtacca gcgtagctga ttttgacgcc
     2461 atgaaccgca gcgctgatat tgtgctttct gatgccaacg tgcgtttagt agtcctctcg
     2521 gcttctgctg gtatcactaa tctgctggtc gctttagcgg aagggctgga acctggcgag
     2581 cgattcgaaa aactcgacgc tattcgcaac atccagtttg ccattctgga acgtctgcgt
     2641 tacccgaacg ttatccgtga agagattgaa cgtctgctgg agaacattac tgttctggca
     2701 gaagcggcgg cgctggcaac gtctccggcg ctgacagatg agctggtcag ccacggcgag
     2761 ctgatgtcga ccctgctgtt tgtcgaaatc ctgcgcgaac gcgatgttca ggcacagtgg
     2821 tttgatgtac gtaaagtgat gcgtaccaac gaccgatttg gtcgtgcaga gccagatgta
     2881 gccgcgctgg cggaactggc cgcgctgcag ctgctcccac gcctcaatga aggcttagtg
     2941 atcacccagg gatttatcgg tagcgaaaat aaaggtcgta caacgacgct tggccgtgga
     3001 ggcagcgatt atacggcagc cttgctggcg gaggctttac acgcatctcg tgttgatatc
     3061 tggaccgacg tcccgggcat ctacaccacc gatccacgcg tagtttccgc agcaaaacgc
     3121 attgatgaaa tcgcgtttgc cgaagcggca gagatggcaa cttttggtgc aaaagtactg
     3181 catccggcaa cgttgctacc cgcagtacgc agcgatatcc cggtctttgt cggctccagc
     3241 aaagacccac gcgcaggtgg tacgctggtg tgcaataaaa ctgaaaatcc gccgctgttc
     3301 cgcgcgctgg cgcttcgtcg caatcagact ctgctcactt tgcacagcct gaatatgctg
     3361 cattctcgcg gtttcctcgc ggaagttttc ggcatcctcg cgcggcataa tatttcggta
     3421 gacttaatca ccacgtcaga agtgagcgtg gcattaaccc ttgataccac cggttcaacc
     3481 tccactggcg atacgttgct gacgcaatct ctgctgatgg agctttccgc actgtgccgg
     3541 gtggaagtag aagaaggtct ggcgctggtc gcgttgattg gcaatgacct gtcaaaagcc
     3601 tgcggcgttg gcaaagaggt attcggcgta ctggaaccgt tcaacattcg catgatttgt
     3661 tacggcgcat ccagccataa cctgtgcttc ctggtgcccg gcgaagatgc cgagcaggtg
     3721 gtgcaaaaac tgcatagtaa tttgtttgag taaatactgt atggcctgga agctatattt
     3781 cgggccgtat tgattttctt gtcactatgc ttatcaataa acgagcctgt actctgttaa
     3841 ccaacgtctt tatcgagaat aattgccttt aattttttta tctgcatctc taattaatta
     3901 tcgaaagaga taaatagtta agagaaggca aaatgaatat tatcagttct gctcgcaaac
     3961 gaattcccgt acactgaact ctctggctca ctgagggcac catcatggca ctcccccgta
     4021 ttacccaaaa agagatgact gaacgcgagc agcgtgaact taaaacactg ctggatcggg
     4081 cgcggattgc gcatggtcgc gtactgacca actccgaaac caacagcatt aagaaagagt
     4141 acatcgataa attaatggtc gagcgtgagg ctgaagcgaa aaaagcccgc caattgaaga
     4201 aaaagcaggc ttataaaccg gatcctgaag cgtcatttag ctggtcagca aatacgtcga
     4261 cgcgcggcag gcgctaatga acaatcaacg cctgagattt tcactcgtca gtgtgaacac
     4321 aattttcccc tcaccctaac ccttttcccg aaggggcgag gggactgtcc gagcactttt
     4381 gggccgtttt catcagtctg aggcgttaat cagcgcccct tctttttacg ccccggcgag
     4441 gtaaaacgct taccgttgga cgccgggcga ccgccttttt ccgccgtttt ttccatctta
     4501 acgactggac gtttgatgcc cgctgttttc ggtttggcct tcgctttggg cttcgcctcg
     4561 gaagaggaat tttcaatgag cttaaagaga tcgataagct cgtcgtcggt taaatcgcgc
     4621 cattctccca gcggaatgcc gcttaagctg acgttcataa tgcgcgtacg ttccagcttt
     4681 ttcacttcat agccgaaatg ctcgcacatg cgccggatct gacggttcaa cccctgcacc
     4741 agggtaatgc ggaagacaaa cggtgcttct tttttgactt tgcacttttt cgtcaccgtc
     4801 ccgaggatcg gcactcccgc ccccatacca cgaataaatt catcggtaat cggtttatcg
     4861 accgtcacca gatactcttt ctcatgatcg ttacccgcac ggaggatctt attcaccaga
     4921 tcgccgtgat tggtgaggaa aatcagcccc tgggagtctt tatccaggcg gccaatcggg
     4981 aacacgcgtt tgctgtggtt aacgaaatcg acaatgttat cgcgctcgcc atcttcggtg
     5041 gtgctgacaa taccgacggg cttgttcagg gcgataagta ccaaatcttc ggcttcccga
     5101 ggctcaatca actgaccgtt tactttcaca atgtcgccgg gtttcacctg atcaccaatg
     5161 gtggctcgct tgccattaag gaacacattg ccttgctcga tatagcgatc cgcttcgcgg
     5221 cgtgagcaaa ttccgctttc gctgatgtat ttatttaaac ggactgatga gtcgggcagc
     5281 ataaattctc ctgtaaaacg cggaatatac cgtacctctt agcggggaaa aacagggtta
     5341 tgcggcaacg tcgaaattaa atgtgatctg ccacgccttt tatcacaaaa gtgtctccgg
     5401 aagtacgatg caaatttgcc tggcaaaata aggataatca acatattacg aatcgctgca
     5461 cacctgtgct gatgactact acgcagagaa acaaatgtgc aagagaaagc gctatttctc
     5521 cccatgcaaa gagtgaaaaa tcgctttgaa aggccctcat aatggggtta tatcagatca
     5581 agcggtgaga aagcgtattt gcacaaatgt gcagaaccag gaggcggcaa tggaaaacag
     5641 tgacgatatc cgtttgattg tgaagattgc ccaactctat tacgaacagg atatgacgca
     5701 ggcgcaaatc gcgcgcgaac tgggtattta ccgcaccacc atcagccgct tgcttaaacg
     5761 aggccgcgat cagggaattg tcaccatcgc catcaactat gactacaacg aaaatctctg
     5821 gctggagcag caactgaagc aaaagtttgg cttgaaagac gttgtggtgg tgtcgggaaa
     5881 tgatgaggat gaagagactc aactggcgat gatggggtta cacggcgcgc aactgctgga
     5941 tcgcttgctg gagcctggcg atattgtcgg tttttcctag ggtcgcgcgg tgagcgcact
     6001 ggttgaaaac ttgccgcaag cggggcaatc gcggcagtta atctgcgtgc caattattgg
     6061 cggaccgtcc ggtaaactcg aaagccgcta tcacgtaaac acattaacct acagcgtggc
     6121 agcgaagctg aaaggggaat cgcatctcgc ggattttccg gctctgctgg ataacccctt
     6181 aattcgtaat gggatcatgc agtctcagca ctttaaaacc atctctgcct actgggataa
     6241 tctggatgtc gccctggtgg gaattggctc accggccatt cgcgacggcg ctaactggca
     6301 tgcgttttat ggtggtgaag agagtgacga cctgaaagca ggcgcggtat tccattggca
     6361 ttgccatgag tgaagaaaaa tacagcggaa ttgttggtgc actgcgtgga aaatatatta
     6421 attgtctggt aacgaatagc agcacagctg aactattact gaaataaaaa tcttcccgat
     6481 gtttaccgta ataaaaatta cggcgggtcc ctattatcta aaatcaggag tctgttatgc
     6541 aaacgtggtt aaatttgcag gataaaatca ttatcgtcac cggcggcgca tccggtattg
     6601 gtctggcgat tgtagaggaa ttattagcac aaggcgcaaa cgtacagatg gtagatattc
     6661 acggtggcga tggtcaatat gaagttcata aaggctatca gttttggccg accgatattt
     6721 ccagcgccaa agaggtaaat catacggtag cggaaattat ccagcgtttt ggtcgcatcg
     6781 acggtctggt caataacgcc ggggtcaatt tcccgcgtct gctggtcgat gagaaagcgc
     6841 ctgccgggca gtatgaactc aacgaagctg cattcgaaaa aatggtcaat atcaaccaaa
     6901 aaggcgtttt tctgatgtcg caggcggtgg cgcgccagat ggtcaaacaa catgatggcg
     6961 tgattgtgaa tgtttcctca gaaagtggac tggaaggctc agaaggccaa agctgttacg
     7021 ccgctaccaa agccgcgctc aatagcttca cgcgctcctg gtcgaaagag ctgggtaaac
     7081 acggtatccg tgtggtcggt atcgcgccgg ggattctgga aaaaaccggg ctacgcacgc
     7141 cggaatatga agaggcgctg gcgtggacgc gcaatatcac cgtcgagcag ctgcgtgaag
     7201 gctataccaa aaacgccatt cctattgggc gcgccggaag attagcagaa gtcgctgatt
     7261 ttgtttgtta tctgctgtct gaacgcgcca gctacatcac cggagtaacc actaacattg
     7321 cgggcggcaa aacgcgcgga taaggaggct ttatggtcaa cgccattttt tgtgcccacg
     7381 gcaaattagc ttgcgccatg ctggaatcgg ttcagatggt gtacggcgac gccaatgtcg
     7441 aggcggttga atttgtacca ggcgagaacg ccggagacat cgtagcaaag ctggaaaagt
     7501 tagtaagcat tcacaatcaa gatgagtggc tgattgccgt ggatttacag tgcggcagcc
     7561 cgtggaatgc ggcagcgatg cttgcgatgc gcaatcctcg tctgcgggta atcagcggcc
     7621 tttccctgcc gctggcgctc gaactggttg ataaccagga cagcatgaat gttgatgaac
     7681 tgtgtgaaca cctgacgcaa atcgcaaagc aaacctgcgt tgtctggaaa caactggcaa
     7741 cggcagagga agatttctga tgaacattac gctcgcccgc attgatgacc gtctgatcca
     7801 cggccaggtc actaccgtct ggtcgaaagt ggctaacgcc cagcgcatta ttatctgcaa
     7861 tgacgaagtt tataacgatg aagtgcgccg gacattattg cgtcaggctg cgccaccggg
     7921 aatgaaggtt aacgtggtca atattgaaaa agccgtcgcg gtttatcaca acccgcaata
     7981 ttaggacgaa accgtttttt atcttttcac tcgcccacag gatgccttag cgatggttcg
     8041 ccagggggta aaaataggca ccctgaatat tggcggcatg gcctggcggc ccggtaaaaa
     8101 acagttaacc aaagcggttt cattagatga tgatgatatt aacgcatttc atgagttaaa
     8161 taatcttggc gtcataatgg atttacgcgt cgtagcgtca gatccctcaa tcaatattat
     8221 cgacaaaata aacgaacagt taatcgcaaa ttaaataata gcgcctgtgt acatatgctt
     8281 taacaggcag ggatgacgac acttaaaagg tgacaaatca tggaaataag taccctacaa
     8341 attattgcta tatttctttt ttcctgtatt gccggaatgg gcagcgtgct ggatgaattt
     8401 cagactcatc gcccgttaat tgcctgtacg gtgattggtt taattctcgg tgatttaaaa
     8461 accggaataa tactcggtgg tacgctggaa ttgatagctc tcggctggat gaacgtcggc
     8521 gcggcgcaat ctccggattc tgcactcgcc agcataatct ccgccattct ggttatcgtt
     8581 ggtcagcaaa gcatcgccac cggaatcgcc atcgcgttgc ctgtggctgc ggcaggccaa
     8641 gtgctgacgg tgtttgcccg taccatcacc gtagtgttcc agcacgcggc ggataaagca
     8701 gcggaagagg cgcggtttcg cactctcgat attctgcatg tctccgcgct tggcgtgcag
     8761 gcgctgcgcg ttgctattcc ggcactgatt gtctcactgt tcgtcagcgc cgatatggtc
     8821 agcaatatgt taaacgccat tcccgaattt gtgacccgtg gactgcaaat tgctggcggt
     8881 tttatcgtgg tggtcggtta cgccatggtg ctacgtatga tgggcgtgaa atatttgatg
     8941 cctttctttt tcctcggttt cctcgcaggt ggctacctcg atctcagcct gctggcgttc
     9001 ggtggcgtcg gcgtgatcat ggccctggtc tacatccagc taaatccaca gtggcgtaaa
     9061 gctgaacctc aactccaggc caccgcctcc accgcccttg accaacttga cgattaacgg
     9121 agccatcatg gaacagagaa aaattacacg cagcgatctg gtgagcatgt ttctgcgctc
     9181 caacctgcaa caggcgtcct ttaactttga acgtattcac ggactgggct tttgctacga
     9241 catgatcccc gccatcaagc gactttaccc attaaaagag gatcaggttg cggcgctcag
     9301 gcgacacctg gtgttcttca ataccacgcc agccgtatgt ggcccggtca tcggcgtcac
     9361 tgccgccatg gaagaggcgc gggccaacgg cgcggaaatt gatgacggta ccattaacgg
     9421 catcaaagtc ggtctgatgg gaccattggc aggagttggc gatccactgg tctggggaac
     9481 gctgcgcccg attaccgctg cgctcggcgc atctctggca ctttctggca acatcctcgg
     9541 cccgctgctg tttttcttta ttttcaatgc ggtgcgtctg gcgatgaagt ggtatggcct
     9601 acagctcggc tttcgcaaag gggtgaatat cgtcagcgat atgggcggga atgtgctgca
     9661 aaaactcacc gaaggcgcgt cgattctcgg actgtttgtg atgggcgtgc tggtcaccaa
     9721 atggacgtca atcaacgtac cgttggtggt ttcacaaacg catgccgccg atggctccac
     9781 cgtcatcatg accgtgcaga acattctcga ccaactttgc cctggtttgc tggcgctcgg
     9841 tctgacgcta ctgatggttc gtctgctcaa caaaaaaatt aacccggtat ggctgatttt
     9901 cgccctgttt ggcttaggga ttatcggcaa tgcgctgggc ttcctgtcct gattcttcgc
     9961 cccggcacga ctgccggggc catcgctcaa catgaggtgg tttatgaaaa caacagctct
    10021 gcgtctttat ggtaaacgtg atttacgcct ggaaaccttt gaccttcctg aaatgcagga
    10081 ggatgaaatc ctcgcgacgg tggtcactga cagcctgtgc ctctcttcct ggaaagaggc
    10141 aacctgggtg aaaaccataa aaaagtaccc gacgatgtgg cgaccaaccc aatcatcatc
    10201 ggccacgagt tttgcggcga tattctggcc gtgggtaaaa agtggcagca caaattccag
    10261 ccgggtcagc gttatgtgat tcaggccaac ctgaaactcc ctgaccgccc ggactgcccc
    10321 ggctactcct tcccgtgggt gggcggcgaa gccacgcatg tggttattcc caacgaggtc
    10381 atggaacaag attgcctgct ggcatacgac ggcgaaacct attttgaagg ctcgctggtt
    10441 gaaccgcttt cctgcgtgat tggcgcgttc aacgccaact atcatcttca ggaaggtagt
    10501 tataaccaca cgatggggat tcgcccgcaa gggcgcacgc tgatcctcgg cggcaccgga
    10561 ccaatgggat tgttggcgat tgattatgcg ctgcatggac cagttaaccc gtcgctgctc
    10621 gtcattaccg ataccgacaa cgacaaattg agttatgcgc gcaagcacta tccatcagaa
    10681 ccgcaaacac tgattcatta tctcaatgcc accgatgcag catttgatac gttaatggcg
    10741 ctgagtggcg gtcacggctt cgatgatatt ttcgtctttg tgcctaatga aggactggtg
    10801 actctcgcct cttccttgct ggcgacagat ggttgcctga atttcttcgc cggaccgcag
    10861 gataaacatt tcagcgcgcc aattaatttc tacgatgtgc attatgcatt tacccactac
    10921 gtgggcacgt caggcggcaa taccgacgac atgcgcgcgg cggtcaaact gattgaagag
    10981 aaaaaagtgc aggccgcaaa agtggtaaca catattcttg ggctgaatgc cgcgggcgaa
    11041 accacgcttg aattgcctgc cgtcggcggc ggcaaaaagc tggtgtatac cgggaaatac
    11101 ctgccgctga cgtcactcac gcagattcag gatcaagcac tggcggcgat tctggcgcgt
    11161 catcagggga tctggtccgg tgaggcggag caatatctgc tcactcatgc agaggcaatt
    11221 tcccatgatt aatcgcgcta cttaactctg catgtcgctg gctggtcgcc gcggcaactt
    11281 cgcgacctga tgtgcggctg attgctgaag cggcccaatg tgcacgagaa ggataaaaaa
    11341 acatcagcgc cttaaggggc gctcaaattg gtggcaaagt acacattgtt tatgccggat
    11401 gcggaataaa gccttattcg gcctacataa tctcgaaaat tcaatatatt gcagttatct
    11461 ggtaggcctg gtaagcgtag cgcatcaggc aatgctgtgt ttgtcatcag tctcagcgcc
    11521 cggtgcaata cgtgacatcg tgagccatgt cagtaagtgg ttaaattcgc agtggtagca
    11581 tgccttccgt gatttcttac taaccggaga agtaatggaa ctgcttttat tgagtaactc
    11641 gacgctgccg ggtaaagcct ggctggaaca tgcactgccg ctaattgctg aacagttgca
    11701 gggtcgccgc tcagcggtgt ttatcccttt cgctggcgta acgcagacct gggatgatta
    11761 cacagcgaaa acggctgcgg ttctcgctcc gctgggtgtt tctgtcaccg gtattcatag
    11821 cgttgtcgat cccgttgccg cgattgaaaa tgctgagatc gtgattgtcg ggggcgggaa
    11881 tactttccag ttgctgaaac agtgccgcga gcgcgggctg ctggcaccaa ttactgacgt
    11941 ggttaaacgt ggtgcgctgt atattggctg gagcgcaggc gctaaccttg cttgcccaac
    12001 cattcgtacc accaacgata tgccgattgt cgatccgcaa ggtttcgatg cgctaaatct
    12061 gttcccgctg caaatcaacc cgcacttcac caacgcgctg ccggaaggcc ataaaggtga
    12121 aacccgtgag cagcgtattc gcgaactgct ggtcgtcgcg ccagaactga cgattattgg
    12181 tctaccggaa ggtaactgga tcacggtgag taaaggtcac gctacgctgg gtggcccgaa
    12241 caccacttat gtgtttaagg ccggtgaaga agcggtgccg ctggaagcgg gtcaccgttt
    12301 ttaagtgaat tgccggatgc atgatccggc atcttgttca tagtgcctga tgcgacgctt
    12361 gacgcgtctt atcaggccta caggattcac aatattaata ctcgtcccgt ccttcatctt
    12421 catccggctg ttccagcaca ctgtaagcca ccgaacaaaa cagcgagttc agacgctgca
    12481 tatctcccag taagcctaaa tgtagcgaac tggtttcaat gctctgcacg ttttgctgat
    12541 gcaggcgatc gacgtgggcg tgggaatagc ggcgattaag aatgcgaaag cgatgcttgc
    12601 tgcggcgcaa acgacgagcg ctggtgacat cgccagagaa gaacaccgac attgccagct
    12661 ttaaattact gagcaattgc tcatagagcg catccagttc cttcaaccca tcaagcgaaa
    12721 atgcccgccg tgctgccagc gatttatcag caatttcgct gcccatgcgc tcgacgatat
    12781 cggaagcctg ttcaaggttg agcgacattt caataatctc cgcccagcga cgcgactctt
    12841 cttctgccag ttcctctttt ggcattcgcg ccagatacag cttaatggcg gtgtagagaa
    12901 cgttgatatc atctgccagc ttacgcagct ctttctcctg ccgtggctcg ccgtgcatca
    12961 ctttattcag cccttccatc atctgttcca tggcgtcgcc aatgcgcagg gtttcgcgcg
    13021 cggcgttcgc cagagcaagc gtgggcgtat ccagcgcgct gacatccaga tgtttaggcc
    13081 gtagctgggt atccagttcc ggttcatcgc gaataatcgt tttgcaaaac cgtgccatcg
    13141 ggtcaacaaa tggcagcatg accaggcaac gtacaaggtt gtagaagacg tggaaataga
    13201 tcaccagttc cgcttttggc aatgacaact tccccattgt ctctgccagc aaatggacaa
    13261 acggcaggat aatcaggcta cccaccagct taaacagcaa actacccagc gcgacacggc
    13321 gggctgcggc attggcggca ctgttgttga gcatcgccag caggccggaa ccgaggttag
    13381 caccaatcac cagacagagc gccacgggga aggagataat gcctgcggcg gtcagagtcg
    13441 cggtcagcag tacagcagca aggctggagt agctgataat ggcgaacatc gcgccaatca
    13501 gcgcatccag cagaatatcg ccggtcagcg aggcaaagat cacctgaacg ccgtttgcct
    13561 gggtgatcgg cgttacggcc tgcacaatca actccagcgc cagcaaaatc agcccaagac
    13621 caataccgac gcggcccagt tgcccggcgc gtgactgttt gcgtccgagg aaaaaaatca
    13681 cgccgataaa aataagtaac ggtgacagcc aggataagtc gaaggtgaga atacgcgcca
    13741 ttagcgccgt cccgacatct gcacccagca caatcaccag agccggtgcg agggctacca
    13801 gatcctgagc gacgaacgag gtcaccagca tggtggtggc attactgctc tgtaccagtg
    13861 cggtaacgcc gatccccgcg caaaaggcga gcggcttttt ttcgacgctc cggctaagga
    13921 cagtacgcaa acgcgcgccg aagacgcgca ttacgccggt tcgaacaata tgagtccccc
    13981 agaccagcag ggcgacggca gaaagcaggt gaagcagcgt taacacgagt tcaggttctc
    14041 cttatcgtta tatgttcctg aagcaattga atgaccaaag gaccggaaac acgctgactg
    14101 gcatctttac tgtgcctttt gacattcgtt tttccggcta agattattcc agcctgtttc
    14161 cagtataagt gtttcatatt aataaagaga cagggcactc attgagcgcc ctgtttgttg
    14221 taaggaaaag tgacagattt gtgaatcagt ccgcgtcata ccccagattc ggtgccagcc
    14281 agcgctcaac atcggagacg ctcatacctt tacggcgggc atagtcttca acctgatcgc
    14341 gctgaatttg cgccaccgcg tagtacttgc tgtccgggtg gctgaaatac caacccgaaa
    14401 ccgatgcacc aggccacatg gcgaaagatt ctgtgagttt catgccagta tgtttttcca
    14461 cttccagcag ctcccagatg gtggcttttt ccgtatgttc cgggcaggcc gggtagcccg
    14521 gtgccggacg gatgccctgg tagttttcgc ggatcagttc ttcgttgctg aggttctcgt
    14581 tcggcgcata gccccagtag actttacgca cacgctcatg gagatactcc gcaaaggctt
    14641 cggctaaacg gtcggcaagc gctttcacca tgattttgtt gtaatcatcg tgctgcgctt
    14701 caaaggcatc agccagtgcg tcctcttcca gcccgccagt cacggcaaat gcgccgatgt
    14761 aatctgcttt accagaaagc ttcggcgcaa cgaagtcagc gagacagtag ttagcgaatc
    14821 ccgttttttc ggtctgttga cgcagatggt ggctgacgtt gatcacatgg gtgcgcgttt
    14881 cgtcacggta gatttcaatg tcatcgccca cacggtttgc cgggaacaag cccaccacgc
    14941 cacgcggatt cagcgttttc tcggcgctta atttatccag catgtcgttg gcgtctttaa
    15001 acagccgctg cgcctcaacg cccaccactt catcttccag aatgcgcgga tacttcccgg
    15061 ccagcgacca ggtcataaag aacggtgtcc agtcgatgta attacgcaga gtttcgatgc
    15121 tggcttcaac ttcctgcacg ccgagacggt gcgccaccgg cggcgtgtaa gcctgccagt
    15181 caaaagcgaa gtcgttatcg cgcgccgctt ccagcgtgac cggtggtgtg cgcggtttct
    15241 tgcgcccgtg ctgaatacgt acggtttcat actccttgcg ggtacgagcg acaaaatcat
    15301 cgcgctgagt atcggaaagc agcgccgcca ccacaccaac ggtgcgcgag gcattctgca
    15361 catacaccgt cgggccgctg tagttctgct caattttcac cgccgtgtgc gcttttgagg
    15421 tcgtcgcgcc accgatcagt aacggaatgg tgaagccctg acgctccatc tctttcgcca
    15481 cgttaaccat ctcgtccagc gacggcgtga taagccccga aagtccaatc agatcagcat
    15541 tcacttcttt agcggtacgg agaatttttt ccgcaggcac cataacgccg agatcgacaa
    15601 tttcgtagtt gttacattgc agcaccacac caacgatatt tttaccgatg tcgtggacgt
    15661 cgcccttcac ggtggcgatc accatcttgc cgttggtttt gccctgctct ttgctggctt
    15721 caataaacgg ttcgaggtag gccactgcct gtttcatgac gcgcgccgat ttgaccacct
    15781 gtggcaggaa cattttcccc tcgccaaaca gatcgccgac cacgttcatg ccgtccatca
    15841 acgggccttc aatcacttca atcgggcgcg tagcctgctg gcgggcttct tcggtatcct
    15901 gctcgataaa ctcagtaatg cctttgacca gcgagtattc cagacgttta ttcacttccc
    15961 acgagcgcca ctccgcctgc tgggcgttag cggtgtcgtc ggttttgctg ccgcgatatt
    16021 tctcggcaag ctccagtaaa cgttcagtgc catcatcgcg acgattaaga atcacatctt
    16081 ccaccgcgtc gcgcagttca gcgggcaggt cgtcgtaaat cgccagttgc ccggcgttga
    16141 cgatccccat atccatgcca ttgcgaatag cgtagtagag gaacaccgcg tgaatggctt
    16201 cgcgcaccgg ttcgttgcca cggaatgaga aagaaacgtt agatacgccg ccggaaatca
    16261 gcgcgtgcgg cagttcgcgt ttgatgtctt cgcaggcgcc gataaagtcc tgcgcatagt
    16321 tgttgtgctc ttcaatgccg gtggcgaccg cgaagatgtt cgggtcgaag atgatatctt
    16381 ctggcgggaa gccaacctct tcggtgagga ttttgtacgc ccgacggcaa atctcgattt
    16441 tccgtgcgcg ggtatcggcc tgtccctgtt cgtcaaaggc cattaccacc accgctgcac
    16501 cgtaacgacg caacagtttc gcatgatgga taaaggcgtc gacgccctct ttcattgaga
    16561 tagagttaac aatgcctttg ccctgaatac acttcagccc cttttcgatg acctcccatt
    16621 ttgaggagtc gatcataatc ggcacgcgag cgatatccgg ttcaccggca atcagattga
    16681 gaaaacgcac catcgccgct tcggcgtcga gcatcccttc atccatattg atatcgataa
    16741 tctgcgcgcc gttttccacc tgctggcggg ccacatccag tgcctcgctg tatttctctt
    16801 ctttgatcag gcgcttgaac ttagcggaac cggtgacgtt ggtgcgttca cccacgttca
    16861 caaacaggct atcttcgcca atgttcagcg gctccaggcc ggacaaacgg caggctacgg
    16921 ggatttccgg cagtttgcgc ggcgctaatc cttctactgc acgactcatc gctgcaatat
    16981 gttgtggcgt ggtgccacag cagccgccga cgatattgag aaagcccgct tccgcccatt
    17041 cacgtatttg ttttgccatc gtgtcggcgt cgagatcgta ctcaccaaag gcgttgggta
    17101 gcccggcgtt cgggtgcgcg gtgacgtagc attccgcaat ccgtgacagc tcctgcacgt
    17161 actggcgcag ttcatcgggc cccagcgcac agttcaggcc aaaggtcagc gcttcggcgt
    17221 ggcgcaatga gttgtaaaat gcttcggtgg tctgcccgga gagcgtgcgc ccggaggcgt
    17281 cggtgatggt gccagagatc ataatcggca gctcaacgcc cagcgtttca aactccgttt
    17341 ttaccgcaaa taccgccgct ttggcgttaa gggtgtcgaa aacggtttca atcaggatca
    17401 gatccgcacc accttccacc agcgctttgg tggactctcg ataagccgcc accagcccgt
    17461 caaaagtgat attacgaaat gccggatcgt tgacgtccgg agaaatagac gccgtgcggt
    17521 tggttgggcc gagaacaccg gcaacgtagc gcggtttctc cggcgtgcgc gcggtccact
    17581 cgtcagcaca ggcgcgcgcc agtttcgccg ccgcaaagtt gatttccgcc gacagggatt
    17641 ccatctggta atccgccatc gcaatggtcg tggagttgaa ggtgttggtt tcgatgatat
    17701 ccgcgcccgc ttcaaagtag gcgttgtgga tagcggcgat cacttccggt ttactgagta
    17761 ccagcaggtc gttgttgcct ttgaggtcgc atggccagtc ggcaaagcgt tcaccacgaa
    17821 aatcggcttc gttcagtcga tagctctgga tcatggtgcc cataccgccg tccagcacca
    17881 gaatacgttc atttaactgc gcacgcagtt gttccacttt gctgctcaca cttgctcccg
    17941 acacgctcaa cccagattta ataaaaattc aacaaaccat actggcataa acgcatctgt
    18001 ggtaaaagcg accaccacgc aacatgagat ttgttcaaca ttaactcatc ggatcagttc
    18061 agcaactatt gcattaacta acaataaaaa tgaaaatgat ttccacgata cagaaaaagg
    18121 agactgtcat ggtcgcaccc attcccgcga aacgcggcag aaaacccgcc gttgccaccg
    18181 caccagcgac tggacaggtt cagtctttaa cgcgtggcct gaaattactg gagtggattg
    18241 ccgaatccaa tggcagtgtg gcactcacgg aactggcgca acaagccggg ttacccaatt
    18301 ccacgaccca ccgcctgcta accacgatgc aacagcaggg tttcgtgcgt caggttggcg
    18361 aactgggaca ctgggcaatc ggcgcacatg cctttatggt cggcagcagc tttctccaga
    18421 gccgtaattt gttagcgatt gttcacccta tcctgcgcaa tctaatggaa gagtctggcg
    18481 aaacggtcaa tatggcggtg cttgatcaaa gcgatcacga agcgattatt atcgatcagg
    18541 tacagtgtac tcacctgatg cgtatgtccg cacctatcgg cggtaaattg ccgatgcacg
    18601 cttccggtgc aggtaaagcc tttttagccc aactgagcga agaacaggtg acgaagctgc
    18661 tgcaccgcaa agggttacat gcctataccc acgcaacgct ggtgtctcct gtgcatttaa
    18721 aagaagatct cgcccaaacg cgcaaacggg gctattcatt tgacgatgag gaacatgcac
    18781 tggggctacg ttgccttgca gcgtgtattt tcgatgagca ccgtgaaccg tttgccgcaa
    18841 tttctatttc cggaccgatt tcacgtatta ccgatgaccg cgtgaccgag tttggcgcga
    18901 tggtgattaa agcggcgaag gaagtgacgc tggcgtacgg tggaatgcgc tgactttttc
    18961 tggcgggcag aggcaatatt ctgcccatca tacctgagtg gcaatagaat aagggtgtct
    19021 gttaatcgca ttgacgccaa aataacttaa tgtcatacac ttcacacact tttattaact
    19081 cagcattatt tttaaacatc aaaccactta attattaaca aaatcgcaag atctcattcc
    19141 accgaaactt ttacggctgc attatgagat tgagcgattt at
//
LOCUS       DAZPDU010000078        18844 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZPDU010000078 DAZPDU010000000
VERSION     DAZPDU010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18844
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.079"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..101)
                     /locus_tag="ACHURS_002416"
     CDS             complement(<1..101)
                     /locus_tag="ACHURS_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1238122.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTA"
     gene            complement(252..2123)
                     /gene="ppiD"
                     /locus_tag="ACHURS_002417"
     CDS             complement(252..2123)
                     /gene="ppiD"
                     /locus_tag="ACHURS_002417"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFR1238123.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(2315..2605)
                     /gene="hupB"
                     /locus_tag="ACHURS_002418"
     CDS             complement(2315..2605)
                     /gene="hupB"
                     /locus_tag="ACHURS_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFR1238124.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(2796..5150)
                     /gene="lon"
                     /locus_tag="ACHURS_002419"
     CDS             complement(2796..5150)
                     /gene="lon"
                     /locus_tag="ACHURS_002419"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFR1238125.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(5338..6612)
                     /gene="clpX"
                     /locus_tag="ACHURS_002420"
     CDS             complement(5338..6612)
                     /gene="clpX"
                     /locus_tag="ACHURS_002420"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFR1238126.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(6738..7361)
                     /gene="clpP"
                     /locus_tag="ACHURS_002421"
     CDS             complement(6738..7361)
                     /gene="clpP"
                     /locus_tag="ACHURS_002421"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFR1238127.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(7607..8905)
                     /gene="tig"
                     /locus_tag="ACHURS_002422"
     CDS             complement(7607..8905)
                     /gene="tig"
                     /locus_tag="ACHURS_002422"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFR1238128.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKRALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(9249..9566)
                     /gene="bolA"
                     /locus_tag="ACHURS_002423"
     CDS             complement(9249..9566)
                     /gene="bolA"
                     /locus_tag="ACHURS_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFR1238129.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            9871..10449
                     /locus_tag="ACHURS_002424"
     CDS             9871..10449
                     /locus_tag="ACHURS_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR1238130.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            10493..11968
                     /gene="ampG"
                     /locus_tag="ACHURS_002425"
     CDS             10493..11968
                     /gene="ampG"
                     /locus_tag="ACHURS_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFR1238131.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPTGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            12431..13378
                     /gene="cyoA"
                     /locus_tag="ACHURS_002426"
     CDS             12431..13378
                     /gene="cyoA"
                     /locus_tag="ACHURS_002426"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFR1238132.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            13400..15391
                     /gene="cyoB"
                     /locus_tag="ACHURS_002427"
     CDS             13400..15391
                     /gene="cyoB"
                     /locus_tag="ACHURS_002427"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFR1238133.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            15381..15995
                     /locus_tag="ACHURS_002428"
     CDS             15381..15995
                     /locus_tag="ACHURS_002428"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFR1238134.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            15995..16324
                     /locus_tag="ACHURS_002429"
     CDS             15995..16324
                     /locus_tag="ACHURS_002429"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFR1238135.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            16336..17226
                     /gene="cyoE"
                     /locus_tag="ACHURS_002430"
     CDS             16336..17226
                     /gene="cyoE"
                     /locus_tag="ACHURS_002430"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFR1238136.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGFAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            17411..17722
                     /locus_tag="ACHURS_002431"
     CDS             17411..17722
                     /locus_tag="ACHURS_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFR1238137.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            17706..18248
                     /locus_tag="ACHURS_002432"
     CDS             17706..18248
                     /locus_tag="ACHURS_002432"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1238138.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNSVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(18304..>18844)
                     /locus_tag="ACHURS_002433"
     CDS             complement(18304..>18844)
                     /locus_tag="ACHURS_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HFR1238139.1"
                     /translation="GIMYANGQYVEVDCKKAKEYLDKGVHIYGGPEDFLATCRKDMID
                     RKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGEVANLRVTFSIRRPSGK
                     EINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFERKSCATVRTTIVAATA
                     TINGKDVELLKAGAIEQKW"
BASE COUNT         4214 a         4958 c         4581 g         5091 t
ORIGIN      
        1 gccgtcggtt tcgccacaga agctgccgcc agcgcgctat gagacattcc ggcacaggcc
       61 agggacaggg taatgagcag tgctttaatt ccgtgtttca tactgttttc tccttgtttg
      121 ttaacagtgg aatcacctta acgggagtga aaacagtaaa caaatggcaa tgttcagaaa
      181 tggaaaaggc cgcgaaagcg accttttctt gttacagctg caacgcattt ttttgcgagg
      241 cggcttcagg attattgctg ttccagcgca tcgccaattt tgattttcgc ctctttacgc
      301 aggttactca tcagagcttc aaagacgatt tgtgcgttgt tctgggtgat accctgcacc
      361 atcgcttttt tctgatcttc cggcattgaa ccttgtttca cttcatccag cgccagcaga
      421 accacattac cttgcatatc ggtcgccata ccgtagctcg gtttgtcttt cgctggcagt
      481 ggcagtgcaa acgccgcctg gctaatcggg tcacgaccgg aacggcttaa ggttttcggc
      541 tcgccaaatt tcagaccggc agcctgcata gcttccgccc ctttgccggc tttcaaatca
      601 accagcagtt tctcagcatc cactttcgcc tgttgttcag ctttgttgtg ctgaaccagt
      661 gccttaactt gttcctgaac atctgccaac ggtttcaccg cttccggttt gtgctcgctg
      721 atgcgcagca cgaatgcgcg gtcgccgtct acggtgatga tgtcagagtt gatgcccggc
      781 gcgccgtttt cacctaccag accgccgtta aagatagcgt cggcaaccgg cttgaagttc
      841 aactcttccg gcaggttatc tttgctgaac caacccgtct gagtggcttt aacgccggca
      901 gcttgctctg caccggccag agactcggtg tcgttgcttg ccgcatcgct cactttctgc
      961 tgcagcgcat agtacgcatc gagggctttt tcgtgtttca ctttcgccgc aatgtcgtca
     1021 cgtacttcgt ctaacgattt cactttcgct ggctgaatgt cgtccagacg tacaatcagg
     1081 aaaccgaccg aagatttgat gacaccagac agttggcctt tttctttcag accagcattt
     1141 ttcagttcgt cggggatagt ggcatcttct aaccaaccca tatcgccgcc gttacgggca
     1201 gagataatat cggcagattt ttctttggct aatgcagcaa aatcaccgcc tttattcagc
     1261 tcatcaagta ccgctttcgc ttcatcttca gttttggtct ggatgatgct gtagcgggta
     1321 cgctgcggct gggtgaattg atcctgatgc tgatcgtagt agctctggat atccgcatcg
     1381 ctaaccggtt gctgcatcgt tgcggcatcc agcttgatgt aactcacgcg gaattgttcc
     1441 ggcgtcatga aattgttttt gttttgttcg tagtagctgg caatctcctg ttcggtcaca
     1501 ggctgcttcg ccgccagcgc gttaacatcg atagtcgcct cacgcaccac gcgttgttgc
     1561 gcgaccagtg ccgccagctc gtcggtttca cctttcagca taaaatcggt accggcaaca
     1621 ccgttaatca gctgttgggt agtgagctgg ttacgcagcg cctgggcgta ctgatcggcg
     1681 gtcatcccca tctggttgag gataccgtta tagcggctgt tatcaaattt gccgtcaacc
     1741 tggaaggctg gggtcgcgaa aatcgcctgt ttaacctgct catcactgat acccagtttc
     1801 agctcacgag cgtactgatc caacagcgcc tcgtcgatca gacgattcag cacctgttga
     1861 cgcagggttt tcatatagcc ttcgttcgct gccagctcgg agtattgatc gcccagctgt
     1921 tgctgcatgc gattacgctc gctgttaaag gcgttctcga actgcccacg gctgatttcc
     1981 tggtcattca cttttgcggc gtagttattg cctccgccaa tcaggtaacc actcacgccg
     2041 gtcaatatga acgacacgat aatgataccg aaaataatct tgagcacgag actgtttgca
     2101 gccgtgcgta agctgtccat catggtgtaa caacactccg ctgtagatga ctgtgtacct
     2161 cacgcaacgt atcgatgcgc gcaaaggcct attgtgacaa gaaacggggg caattgtcag
     2221 cccacaagac acagaaagtc accgaataca aataaaaaag gcacatcagt agatgcgccc
     2281 ttgaacttcg tcacatcccc actggggaca acgcttagtt taccgcgtct ttcagtgctt
     2341 tacctgcacg gaagctcggt actttagcgg cagcgatggt gatctcttta ccggtctgcg
     2401 ggttgcggcc agtacgggca gcacgctctt taacggcaaa agtaccaaaa cctaccagtg
     2461 ctacatcatc cccttctttc agagattcag ttacggaagc aataatagca tctaacgcac
     2521 ggccagccgc agctttagag atatcagccc ctgcagcaat cttgtcgatc aattgagatt
     2581 tattcactct tctcttcctc tttataattt atatcgcact tgaatccttc aaggtacgaa
     2641 cgcgcagcag ttatatcagg ccagccatcc ccttacaaca ccccttaata agggcaagcc
     2701 cgatccgcca tctaacttag cgagacaaaa aaaggctggc aagcccgaat tagcctgcca
     2761 gccctgtttt tattagtgca ttttgcgcga ggtcactatt ttgcagtcac aacctgcatg
     2821 ccagacggtt cattttgcag cgccagagtc agaacttcct caatgcgctt cacaggatga
     2881 atgtccagat cggcaattac gttgtcagga atctcttcca gatcgcgttt attttcgaac
     2941 ggaattagca ctgttttaat cccgccgcga tgcgctgcca ggagtttttc tttcaaacca
     3001 ccgatcggca gtacctgacc acgcagagtg atctcaccgg tcattgccac atcggcacga
     3061 accgggttac cggtcaggca agaaaccagc gcggtgcaca tagcaatacc ggcactcgga
     3121 ccatctttcg gcgtcgcacc ttccggtacg tggacgtgga tgtcacgttt ttcgtaaaaa
     3181 tcagggttga tccccagttt ttccgcacgc gcacgaacca ccgttaacgc cgcctgaata
     3241 gactcctgca tcacttcgcc gagcgaaccg gtataggtca gtttgccttt acccggaaca
     3301 catgcggttt caatggtcag caagtcaccg cccacttccg tccacgccag accggttacc
     3361 tgaccgacac ggttttcgtt atcagcgcga ccatagtcga aacgctgaac accgaggtag
     3421 tcatgcaggt tatcgccgtt aatttcgata tgttttaatg acttatcgag cagtaactgc
     3481 ttaaccgctt tgcggcacag tttggagatt tcacgctcca gaccacgcac gcccgcctca
     3541 cgggtgtagt aacgaataat gccgataatg gcgctatcgt cgacggtcag ctcacctttt
     3601 ttcagtgcat tacgttcaat ctgcttcggc agcaggtgac gtttggcgat gttcagtttt
     3661 tcatcttcgg tataaccgga gaggcgaatc acttccatac gatccagcag cggtgccgga
     3721 atgttcatgg agttcgacgt cgcgacaaac atcacgtcgc tgagatcgta atccacttcc
     3781 aggtagtggt cgctgaacgc tacgttctgc tctggatcca gcacttcaag cagtgcagag
     3841 gccggatcgc ctcgcatgtc agaagacatt ttgtcgatct catcgagcag gaacagcggg
     3901 tttttcacgc ccactttcgc cattttctgg atcagtttac ccggcataga accgatgtaa
     3961 gtacggcggt gaccacggat ttccgcttca tcacgcacgc cgcccagcgc catacggaca
     4021 tatttacgcc cggtggcttt ggcaatggac tgaccaagag aggttttacc tacccccggc
     4081 ggccctacca ggcagaggat cggtcccttg attttgttga cacggctttg aaccgcaagg
     4141 tactcaagga tgcgatcttt cacgcgctcc agaccataat ggtcggtatc aaggatttcc
     4201 tgcgcctgac gcaggtcttt tttgaccttg ctgcgcgcat tccacggtac ctgtaccatc
     4261 cagtcgatat aaccacgcac tacggtcgct tctgccgaca tcggagacat cattttcagc
     4321 ttctgcaact ctgcttccgc tttctctttt gcctctttcg gcatcttcgc cgcgtcgatt
     4381 ttgcgcttca gggcttcgtt ttcgtccggc gcgtcgtcca tttcaccgag ttctttctga
     4441 atagctttca tttgctcgtt cagatagtac tcacgctggg atttctccat ctgcttttta
     4501 acgcggttgc gaatgcgttt ctcaacctgc agcagatcga tttccgattc catcattgcc
     4561 atcagatatt ccagacgttc gttaacgtcg gacatctcca gaacggactg tttgtcagcc
     4621 agtttcagcg gcatatgtgc agcaatggta tccgccagac gcgccggatc gtcgatgcta
     4681 ttcagcgacg tcagcacttc tggtgggatt tttttgttca gcttgatgta gccttcgaac
     4741 tggctgattg cagtacgcac cagcacttcc tgttcccgct catcaatggt cggcgactcc
     4801 agatactccg ccttcgcaga aaagtgttcg ccattgtcag agagcgcaga aatacgcgcg
     4861 cgctgtaacc cctcgaccag cactttgacg gtgccgtcag gcagtttcag catctgcaat
     4921 atagaggcca cggtcccgac ggtgaaaaga tcgtttacac ccggctcatc cgttgaagct
     4981 tctttctgcg cgaccagcat aattttttta tcatggtcca tcgccgcttc cagacaacgg
     5041 atagattttt cccgcccgac aaataagggg atgaccatgt gcggataaac caccacatcg
     5101 cgcagcggca atacggggat ttcaatgcgt tcagaacgct caggattcat agagctctct
     5161 cttagtttaa tttccgccag gtaatcagat gacacgactg tgcttcacgc catctattaa
     5221 catgtacgtc agtatatggg gatgtttccc ccacattcaa cgccgagaat agaggaaaaa
     5281 ttaaagggga gataaaatcc cccctttttg gttaactaat tgtatgggaa tggttaatta
     5341 ttcaccagat gcctgttgcg cttccggctt accataaatc agcaacggtt tgctttgacc
     5401 atcaattacc gactcgtcga taaccacttt ttcgacgtct tccatggacg gcagatcgta
     5461 catggtatcg agcagtgcgg cttctacgat ggaacgcagg ccacgggcac cggttttacg
     5521 cgccatcgct ttcttagcga tagcatccag cgcctcgtca cggaattcca gatccacgcc
     5581 ttccagatta aacagcgcct gatactgctt ggtcagggcg tttttcggct ctttgaggat
     5641 ctgaatcaga gcttcttcgc tcagttcatt caacgttgct acaaccggca gacgaccaat
     5701 gaactcaggg ataagaccaa acttgatcag atcttccggt tcaacctgcg ccagcagctc
     5761 gccttcgctt gctttgtcgg acttcgcttt taccgtcgcg ccaaaaccaa tgccggagcc
     5821 ggtttctaca cggtgggaaa tcactttatc cagaccggca aacgcaccgc cacaaataaa
     5881 caggatctta gaggtatcaa cctgcaagaa ttcctgctgc ggatgtttac gcccaccttg
     5941 cggtggaaca gcagctaccg taccttcgat cagtttcaac agtgcctgct gtacgccttc
     6001 accggaaacg tctcgggtaa tggacgggtt gtctgactta cgagaaatct tgtcgatttc
     6061 atcgatgtag acaatcccgc gctgcgcttt ctgtacgtcg taatcgcact tttgcaacag
     6121 cttctgaatg atgttttcaa cgtcttcgcc cacataaccg gcttcggtca gcgtggttgc
     6181 gtcggccatg gtgaacggga cgtccagcag gcgcgccagc gtttcagcca gcagcgtttt
     6241 accggaaccg gtcggaccga tcagcagaat gttacttttg cccaactcga caccattgct
     6301 ggtatcgccg ttgcgcagac gtttgtaatg gttgtatacc gcgaccgcca gcactttttt
     6361 cgcctgttcc tggccgataa cgtaatcgtc caggtggttg cgaatttcat gcggcgtcgg
     6421 tagcgcactg cgttcacgat gcggtgcaac ttctttaatc tcttcgcgaa tgatgtcgtt
     6481 acataaatca acacattcgt cgcagatata cacggatgga ccggcaatca gcttgcgcac
     6541 ttcatgctgg cttttgccgc aaaaagagca atacagcaat ttgcctgagc catctttgcg
     6601 tttatctgtc atgggtcaaa acctcttctt tgttctttgt gccgcacacg acgacgcaaa
     6661 tgccattctc aggcgcaagc cgcttacagc gttgtgccgc cctggataag tatagcggca
     6721 cagttgcgcc tctggcatca attacgatgg gtcagaatcg aatcgaccag accgtattcc
     6781 accgcttcag gggcggaaag gaagcgatcg cgctcggtat cacgttcaat ctgttctaat
     6841 gattgacccg tatgaagcgc cataagttca ttcatgcgcc ctttaacttt cagaatttca
     6901 cgggcatgaa tttcgatatc ggtcgcctgg ccctggtagc cgcccaacgg ttggtgaatc
     6961 atcacgcgcg aattcggcag gcaaaaacgt ttaccttttg cccctgcggt cagcaagaaa
     7021 gcgcccatcg aggccgcctg gcccatacag atggtgctga catcaggctt gataaactgc
     7081 atggtgtcat agatagacat cccggcagtg atcaccccgc ctggggagtt aatgtacaga
     7141 tagatatctt tttctgggtt ttccgcttct aggaacagca tctgcgccac aatcaggttc
     7201 gccatgtggt cttcaacctg gccagtcaga aaaatgacgc gttccttaag tagacgagaa
     7261 tagatatcaa aagagcgctc accgcgtgag gtctgttcaa tgaccatcgg caccagcgcc
     7321 atatggggtg caaagttatc tcgttcgccg ctgtatgaca tttccgtctc ctggataaaa
     7381 ttgaaaaaac ctgctgtacc gattgtaacc ttatggacgg attatcagct agtccctgtg
     7441 ttatgggtac ggcatcacca tatttcaagc ataacaatct tttgttgtta cgctaacacc
     7501 gaaagaggct tttcgcacgg ttccatgcaa aattcatgac aaaaaaaagc ccaccacctg
     7561 gcggtgacgg gcctttgtgc gaatttagcg cgttatgctg cgtaaattac gcctgctggt
     7621 tcatcagctc gttgaaagtg gtttcttttt cagtcacttt cgctttcgcc agtacagctt
     7681 caacagcctg ttcttccaga gcaacattgc gcatgttgtc catcagttct ttgtttttgc
     7741 tgtagaactc gataacttct ttcggatctt cgtacgcaga agccatctct tcgatcaggc
     7801 ctttcacgcg ctcttcgtca gctttcagct cgttggtgcg gataacttcg cccagcagca
     7861 ggccaacaac tacgcggcgt ttagcctgtt cttcgaacag ttcgcgcggc agttccagag
     7921 ctcgtttttc gttgccaccg aaacgctgtg cagcctggcg acgcagaacg tcgatttcgc
     7981 tgtcgatcag cgcagccggt acgtcgatgt cgttagcttt taccagacct tcgatcgcct
     8041 gagacttaac gcggttacgg atggcgctct tcagctcgcg ctccatgttt ttacgcactt
     8101 cagcgcgcag accttctacg gaaccatctt caacgccgaa acgtttgatg aattctgcag
     8161 tcagttccgg cagttcacgc tcttcaactt tcttcaggtt gatagcgaat ttcgctgctt
     8221 tacctttcag attttctgcg tggtattctt ccgggaaggt cacgtcgatg gtgaactctt
     8281 cgccagcttt gtggcctttg ataccgtctt caaagcccgg gatcatacga ccctggccca
     8341 tcgccagtac gaaatcagac gctttaccgc cttcgaactc ttcgccgtct acagaaccgg
     8401 tgaagtcgat ggttacgcgg tcttctgctt caacggcgcc gtctttttct ttccaggtcg
     8461 cctgctgttt acgcagagta tccagcatgc cgtcaacgtc agcgtcggtc acttcaacga
     8521 tcggtttttc aacttcgatc gcttccagac cctgcagttc aacttccgga taaacttcaa
     8581 actctacaga gtaggtgaag tcttcaccca gcttgtattc gcccggaaca taagtcggtg
     8641 cgccagccgg attgattttt tctttaatga tggcgtcaat gaagttacgg ctcatcaggt
     8701 cacccagaac gtcctggcgt acagacgcgc cataacgctg agcaacgata ttcattggca
     8761 ctttgccttt gcggaagccg tcaatacgta cttttttcgc aacgttgacc agctcgcttt
     8821 taacagcggt ctcgatgctg tcagcagcga tagtaatcgt tacacggcgg ccaaggcctt
     8881 gagtggtttc aactgaaact tgcatcttgt tacctcaaaa aatcacagtg ctcggtcaac
     8941 tcttaccccg caagcacagg ttgttggctt cgcggaaccg ggatgttctt ataatcaatc
     9001 acattccctg ttaccagaat catcccgaaa gcattcaaat aggacgcggc attatagcgg
     9061 catcactttt atgagtcgag aacggttatt gcgcgctgtc gcgtattttt tcacaattcc
     9121 cgggcaattt tcgtctaaat gctgggcaaa aagcgcaaaa tccagacaaa acaaaacggc
     9181 ccgcaggccg tttttcataa aatctttagc aacatactgg aaaagcgccg acagttgcaa
     9241 atgcgttttt acgcgatgct tcctgctcca cgacagggag gagaggcaaa gacggtgtcc
     9301 tgcaaccctt cccactcctt aatagtgtaa gtatgcagag ccagcgcatg aacggtagta
     9361 gagagttcct ccgctaaagt actgtaaatc attcgatgac gattcagaaa acgttcaccc
     9421 gtaaaacgat cgctgaccag cacaacttta aaatggcttt cagagccggc tgggacattg
     9481 tgacgatagc tttcatccac tacttcgagg aatacgggtt ggaacgccgc ccttaatttt
     9541 tcttctatcc gctcacgtat catcatgaaa tatctcctcc gacaacgctg agatgtcatc
     9601 catcccttta aatactagcc gcttttaccg tttccattgc agcttaacaa caaatattta
     9661 ggtttcgtcc tggtttttcc gtcaagcgta tgaagtttac tgatttaaag attgaaacac
     9721 aggcctcgca tcagcgtttc ttttaccagg ggtgattatt tcgccaacat gatgaattta
     9781 catgccttac ccacttcccc tcgccgttgg caatgttatg atggcgggaa tttttaccaa
     9841 acacgcttaa gagtctctga gagcccgaac atgttcaaaa aaatcctctt cccgttagtt
     9901 gctctgttta tgcttgcagg atgcgcaaaa ccgccaacaa ctattgaagt ttccccgacg
     9961 attacactgc cacagcagga tccaagcctg atgggcgtca ccgtaagcat taatggtgcc
    10021 gatcagcgta ccgatcaggc gctggcaaaa gtcacccgcg ataatcaaat cgttaccctg
    10081 accgcctccc gagatctgcg tttcctgctg caagaagtgc tggaaaaaca gatgaccgcg
    10141 cgtggttaca tggttggtcc gaatggcccg gttaatctgc aaatcatcgt tagccaactg
    10201 tatgctgacg tgtcccaggg caacgtgcgc tacaacatcg cgaccaaagc ggatatcgcc
    10261 atcatcgcta ccgcgcagaa tggcaacaaa atgaccaaaa actatcgtgc cagctacaac
    10321 gttgaaggtg cgttccaggc ctccaacaaa aatatcgccg atgcggttaa cagtgtgctg
    10381 accgatacca tcgctgatat gtctcaggac accagcatcc acgaattcat caagcagaac
    10441 gcgcgttaat ttctgccctc tggcccggtg caaaccgggc ctgtagacgc ccatgtccag
    10501 tcaatattta cgtatttttc aacagccgcg ttcagccata ttgctgatcc tgggttttgc
    10561 ttccgggcta ccgctcgccc tgacatccgg taccttacag gcctggatga cggtcgagaa
    10621 tattgatctc aaaaccattg gtttcttctc tctggtaggc caggcttacg tttttaaatt
    10681 cctctggtca ccgctgatgg accgctacac gcccccattt tttgggcggc ggcgcggttg
    10741 gctgctcgcc acgcaaatcc tgttattagt cgccattgcg gcgatgggtt ttctcgaacc
    10801 tggcacccaa ctccgctgga tggcggcact ggcggtggtg atcgcttttt gctcggcctc
    10861 ccaggatatt gtcttcgatg cgtggaaaac cgatgtgctc ccggcagaag aacgtggtgc
    10921 gggcgcggca atcagcgtgc tgggttaccg tttagggatg ctggtttccg gcggcttagc
    10981 cctgtggctg gcagataaat ggctgggctg gcagggcatg tactggttga tggcggcgct
    11041 gttgatccct tgtattatcg cgacgttgct tgcaccagaa ccgaccgaca ccattcctgt
    11101 gccaaaaacg ctggaacaag cggttgttgc acctctgcga gatttctttg gtcgcaataa
    11161 tgcctggctt attttgcttc ttatcgtgct gtataagctg ggcgacgcat tcgccatgag
    11221 cctgacaacc acgtttttga ttcgcggcgt cgggtttgat gcgggtgaag taggcgtggt
    11281 taacaaaacg cttggcttac tggcgaccat tgtgggcgca ttgtacggtg ggattttgat
    11341 gcagcgcctg tcgctgttcc gggcgctgct gattttcggc attttacaag gtgcgtctaa
    11401 cgctggttac tggctgctgt cgattactga taagcatctc tacagcatgg gcgcagccgt
    11461 ctttttcgaa aacctctgtg gcgggatggg cacatcagcc tttgtcgcgc tgttaatgac
    11521 gctgtgtaat aagtcatttt ccgctactca atttgccctg ctctcagcgc tttccgctgt
    11581 aggacgagtt tatgtcggcc ccgtggcggg ttggtttgtt gaagcacacg gctggtcgac
    11641 attctatcta ttctccgtcg ccgctgccgt accggggctt attttgctgc tggtttgccg
    11701 ccagacgctt gaatatacac gagtaaatga caactttatc tcccgtaccg aatatccgac
    11761 aggttatgcc tttgccatgt ggacactggc ggcgggcgtc agcctgttgg ccgtgtggtt
    11821 actgctgttg acgatggacg cgctggattt gacgcacttc tctttcctgc ctgctctgct
    11881 ggaagtcggg gttttagtcg ccctttctgg cgtcgtgctt ggtggtttgc tggattatct
    11941 ggctctacga aaaacgcatc tgacgtaatc tgtaaatatt atttagctgt agtaatcact
    12001 cgccgtaatt attacggcta aataaacatc agtatcgctt aataatattt gttcttttgt
    12061 gcggcttagc gtttggaaat tattgacgca atttatgaat tctatttttt cttacacgat
    12121 tcagctaatg agtctttatt ttcccccccc acccaattgt caccataatg ataattattt
    12181 gttaaacaat tgttttattc cagattggtt ataccaattg cccgtccaaa ctccttctgc
    12241 actccccttt cgttataacg cccttttgca acagcttctt aaaatcaact tgatatgttt
    12301 tgcaacatat gtaacctgac agccaaatcc aagtaacagg aatttaatca tgtttacagt
    12361 aatgcaacct ccccgtaaaa tgcccacaca ctttaaacgc caccagatcc cgtggaattg
    12421 aggtcgttaa atgagactca ggaaatacaa taaaagtttg ggatggttgt cattatttgc
    12481 aggcactgta ttgctcagtg gctgtaattc tgcgctgtta gatcccaaag gacagattgg
    12541 tctggagcaa cgttcactga tactgacggc atttggcctg atgttgattg tcgttattcc
    12601 cgcaatcttg atggctgttg gtttcgcctg gaagtatcgt gcgagcaata aagatgctaa
    12661 gtacagcccg aactggtcac actccaataa agtggaagct gtggtctgga cggtacctat
    12721 cttaatcatc atcttccttg cggtactgac ctggaaaacc actcacgctc ttgagcctag
    12781 caagccgctg gcacacgacg agaagcccat taccatcgaa gtggtttcca tggactggaa
    12841 atggttcttc atctacccgg aacagggcat tgcaaccgtg aatgaaatcg ctttcccggc
    12901 gaacactccg gtgtacttca aagtgacctc caactccgtg atgaactcct tcttcattcc
    12961 gcgtctgggt agccagattt atgccatggc cggtatgcag actcgcctgc atctgatcgc
    13021 caacgaaccc ggtacttatg acggtatctc cgccagctac agcgggccgg gcttctcagg
    13081 catgaagttc aaagctattg caacaccgga tcgcgccgaa ttcgaccaat gggtcgcaaa
    13141 agcgaaacag tcgccgaaca ccatgtctga catggctgcg ttcgaaaaac tggccgcgcc
    13201 tagcgaatac aaccaggtgg aatatttctc caacgtgaaa ccagacttgt ttgccgatgt
    13261 gattaacaag tttatggctc acggtaagag catggacatg acccagccag aaggtgagca
    13321 cagtgcacac gaaggtatgg aaggcatgga catgagccac gcggaatccg cccattaaag
    13381 gggttgagga agaataaaga tgttcggaaa attatcactt gatgcagtcc cgttccatga
    13441 acctatcgtc atggttacga tcgctggcat tattttggga ggtctggcgc tcgttggcct
    13501 gatcacttac ttcggtaagt ggacctacct gtggaaagag tggctgacct ccgtcgacca
    13561 taaacgcctc ggtatcatgt atatcatcgt ggcgattgtg atgttgctgc gtggttttgc
    13621 tgacgccatt atgatgcgta gccagcaggc tcttgcctcg gcgggcgaag cgggcttcct
    13681 gccacctcac cactacgatc agatcttcac cgcgcacggc gtgattatga tcttcttcgt
    13741 ggcgatgcct ttcgttatcg gtctgatgaa cctggtggtt ccgctgcaga tcggcgcgcg
    13801 tgacgttgcg ttcccgttcc tcaacaactt aagcttctgg tttactgttg ttggtgtgat
    13861 tctggttaac gtttctctcg gcgtgggcga atttgcgcag accggctggc tggcctatcc
    13921 accgctatcg ggaatagagt acagtccggg agtcggtgtc gattactgga tatggagtct
    13981 ccagctatcc ggtataggta cgacgcttac cggtatcaac ttcttcgtta ccattctgaa
    14041 gatgcgcgca ccgggcatga ccatgttcaa gatgccagta tttacctggg catcactgtg
    14101 cgcgaacgta ctgattattg cttccttccc aattctgacg gttaccgtcg cgttgttgac
    14161 cctggatcgc tatctgggca cccatttctt taccaacgat atgggtggca acatgatgat
    14221 gtacatcaac ctgatttggg cctggggcca cccggaagtt tacatcctga tcctgcctgt
    14281 tttcggtgtg ttctccgaaa ttgcggcaac cttctcgcgt aaacgtctgt ttggttatac
    14341 ctcgctggta tgggcaaccg tctgtatcac cgtgctgtcg ttcatcgttt ggctgcacca
    14401 cttctttacg atgggtgcgg gcgcgaacgt aaacgccttc tttggtatca ccacaatgat
    14461 tatcgccatc ccgaccgggg tgaagatctt caactggctg ttcaccatgt atcagggccg
    14521 catcgtgttc cattctgcga tgctgtggac catcggtttt atcgtcactt tctcggtggg
    14581 cgggatgact ggcgtgctgc tggcagtacc gggcgcggac ttcgttctgc ataacagcct
    14641 gttcctgatt gcgcacttcc ataacgttat catcggcggc gtggtcttcg gctgcttcgc
    14701 agggatgacc tactggtggc ctaaagcgtt cggtttcaaa ctgaatgaaa cctggggtaa
    14761 acgcgcgttc tggttctgga tcatcggctt cttcgttgcc tttatgccgc tgtatgcgct
    14821 gggcttcatg ggcatgaccc gtcgtttgag ccagcagatt gacccgcagt tccacaccat
    14881 gctgatgatt gcagctagcg gtgcagtact gattgcgctg ggtattctct gcctcgttat
    14941 tcagatgtac gtttctattc gcgaccgcga ccagaaccgt gacctgactg gcgacccgtg
    15001 gggtggccgt acgctggagt gggcaacctc ttccccgcct ccgttctata actttgccgt
    15061 tgtgccgcac gttcacgagc gtgatgcatt ctgggaaatg aaagagaaag gcgaagcgta
    15121 taaaaagcct gaccactatg aagaaattca tatgccgaaa aacagcggtg caggtatcgt
    15181 cattgcagct ttctccacca tcttcggttt cgccatgatc tggcatatct ggtggctggc
    15241 gattgttggc ttcgcaggca tgatcatcac ctggatcgtg aaaagcttcg acgaggacgt
    15301 ggattactac gtgccggtgg cagaaatcga aaaactggaa aaccagcatt tcgatgagat
    15361 tactaaggca gggctgaaaa atggcaactg atactttgac gcacgcgact gcccacgcgc
    15421 acgaacacgg gcaccacgat gcaggcggaa ccaaaatctt cggattttgg atctacctga
    15481 tgagcgactg cattctgttc tcaatcttgt ttgctaccta tgccgttctg gtgaacggca
    15541 ccgcaggcgg cccgacaggt aaggacattt tcgaactgcc gttcgttctg gttgaaactt
    15601 tcttgctgtt gttcagctcc atcacctacg gcatggcggc tatcgccatg tacaaaaaca
    15661 acaaaagcca ggttatctcc tggctggcgt tgacctggtt gtttggtgcc ggatttatcg
    15721 ggatggaaat ctacgaattc catcacctga ttgttaacgg catgggtccg gatcgcagcg
    15781 gcttcctgtc agcgttcttt gcgctggtcg gcacgcacgg tctgcacgtc acttccggtc
    15841 ttatctggat ggcggtgctg atggtacaaa tcgctcgtcg cggcctgacc agcactaacc
    15901 gtacccgcat catgtgcctg agcctgttct ggcacttcct ggatgtggtt tggatctgtg
    15961 tgttcactgt tgtttatctg atgggggcga tgtaatgagt cattctaccg atcacagcgg
    16021 cgcgtcccat ggcagcgtaa aaacctacat gacaggcttt atcctgtcga tcattctgac
    16081 ggtgattccg ttctggatgg tgatgacagg agctgcctct ccggccgtaa ttctgggaac
    16141 aatcctggca atggcagtgg tacaggttct ggtgcatctg gtgtgcttcc tgcacatgaa
    16201 taccaaatca gatgaaggct ggaacatgac ggcgtttgtc ttcaccgtgc taatcatcgc
    16261 tatcctggtt gtaggctcca tctggattat gtggaacctc aactacaaca tgatgatgca
    16321 ctaagagcgg cggttatgat gtttaagcaa tacctgcaag taacgaaacc aggcatcatc
    16381 tttggcaacc tgatctcggt gattggggga ttcctgctgg cctcaaaggg cagcattgat
    16441 tatcccctgt ttatctacac gctggttggg gtgtcactgg ttgtggcgtc gggttgtgtg
    16501 tttaacaact acatcgacag ggatatcgac agaaagatgg aaaggacgaa gaatcgggtg
    16561 ctggttaaag gcctgatctc tcctgctgtc tcgctggtgt acgccacctt gctaggcttt
    16621 gctggcttta tgctgctgtg gtttggcgcg aatccgctgg cctgctggct gggggtgatg
    16681 ggctttgtgg tttatgtcgg cgtttatagc ctgtacatga aacgccactc ggtctacggc
    16741 acattgattg gttcgctctc cggcgctgcg ccgccggtga ttggctactg tgcagtaacc
    16801 ggcgagttcg atagcggcgc agcgatcctg ctggctatct tcagcctgtg gcagatgcct
    16861 cactcctatg ccatcgccat tttccgcttt aaggattatc aggcggcaaa cattccggta
    16921 ttgccagtgg taaaaggcat ttcggtggcg aagaatcaca tcacgctgta tatcatcgcc
    16981 tttgccgttg ccacgctgat gctctctctt ggcggttacg ctgggtataa atatctggtg
    17041 gtcgccgcgg cggtgagcgt ctggtggtta ggtatggccc tgcgcggtta taaagttgct
    17101 gatgacagga tctgggcgcg caagctgttc ggcttctcta tcatcgccat cacagccctc
    17161 tcggtgatga tgtccgttga ttttatggtg ccggactcgc atacgctgct ggctgctgtt
    17221 tggtaacaca acctctctat taaaaaggtg ctacggcacc ttttttattg tctgagatca
    17281 gagatacggc taataaccgt atgtcttgac agagtgagca gatgaacata aattaatcat
    17341 acggttgata gccgtattat gtatgagatc gactccctac gatttcattc agttaatcaa
    17401 agaggtcata atgcctggaa aaacagccac tctacccgat gtcgataaaa cgctgaaaaa
    17461 tgcgcgggtc gaactcaaaa ctagtcccga cgccaaaaac aaacttcgtg aagctgcgca
    17521 agccgttggg gtagatttaa gcgcttttat tttgagcgct gctatggaac gcgccgaaag
    17581 tgtgcttgat aaccaacgtc gccgtgagct ttcgaatcaa agctgggaac tgatgaacca
    17641 actcatcgct gaacctgctc aaccgacgct cgccctcaag gcgttaatga aaaggaaaaa
    17701 cagcgatggt cgacaagcat gaagagatta ctctgcccat agtcctctcc tgtaattatc
    17761 agtctgatat tacttatcct gggcaaaaac agtttgattg cggtaactct gttatcgata
    17821 aatttgtacg cgcatcgcta aagaaaagtg tgcgtaatag cgactgtgcg gctaaagcac
    17881 ttattgacag acaaagtggt gaactgatcg gcatctgtac ttttacggca tattcgctgg
    17941 aaaaacaacg cgtttctggc gtccttcagg gttcacaacc ttcagaaatt ggtgttgtca
    18001 gattagtcat gttgggggta gcacggaagt atcaaaagcg gggctttggt caggacctac
    18061 tatgtgattt ttttgaacat gtaaaaataa ttcaccaggc attaccaatt aaaggggttt
    18121 atcttgatgc tgaccctgcc gccattaatt tttatgctcg tctcggcttt gttcagcttt
    18181 cagcgacacc aaatgctttt ggtgctgtac ctatgttttt ggcgattcag catattctcg
    18241 cggcttagat tattatcgcc acccgccagg gcaagttttg ccttagccgg gggctgagca
    18301 gcgttaccac ttttgttcaa ttgcccccgc tttcagcaac tccacatcct tgccgttaat
    18361 ggttgccgtt gcggcaacga ttgtggtcct tacagttgca caagattttc gctcaaattc
    18421 cggtttataa agaataagag acgaattact ggtaaatgaa ccaaataggt aatcagtaaa
    18481 gctaatattc agccggttta aaccgaaagg cgcgaagcca accgtttggt taatctcctt
    18541 accggatggg cgacgaatgc tgaatgtcac gcgaagattg gccacttcgc ctaatttatt
    18601 ggtggtggcg aagaggctat ccatgcagga aaaaccttta tctaaaaaat tatctcgcat
    18661 cccagaacgg gtaaccgtaa tcacgggtaa cgtatcgtca accgtttttc tgtcaatcat
    18721 gtcttttcga caagtagcca gaaagtcttc cgggccacca taaatgtgga cacctttatc
    18781 aagatattcc ttcgctttct tacaatctac ctcaacatac tgaccattgg cgtacataat
    18841 gccc
//
LOCUS       DAZPDU010000079        18662 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZPDU010000079 DAZPDU010000000
VERSION     DAZPDU010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18662
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.080"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            54..1178
                     /locus_tag="ACHURS_002434"
     CDS             54..1178
                     /locus_tag="ACHURS_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000486721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFR1238140.1"
                     /translation="MFQKIKIRKMTISRALDKYLKTVSIHKKGHLQEFYRVNVIKRHP
                     IAERYMDDITTVDIANYRDQRLAQINPRTGRQITGNTVRLELALLSSLFNIARVEWGT
                     CRMNPVELVRKPKISSGRDRRLTSGEERRLSRYFKEKNQALYVIFHLALETAMRQGEI
                     LSLRWEHVDLQHGVAHLPTTKNGAPRDVPLSRKARNYLQMLPTQLNGNIFSYTSSGFK
                     SAWRTALQELKIENLHFHDLRHEAISRFFELGTLNVIEVAAISGHRSLNMLKRYTHLR
                     AYQLVSKLDARRKQTSKIAPYFVPYPATVENRNGQVVVTLSDFDLETSAATKEQAIFH
                     ASVLLLRTLAQAAQRGERVPTPGELPTNIDERVMICPLTN"
     gene            1190..1843
                     /locus_tag="ACHURS_002435"
     CDS             1190..1843
                     /locus_tag="ACHURS_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1238141.1"
                     /translation="MIEQLQSDFLFWIFIYFLIVGLLIRNIHLTRKIKSIEKNKMFPA
                     EEYAVLDIEQHMSNKYIGRLVTRFMKRTNGAPLLIVFPEYDKGDNNEDKNSPPFQLVK
                     LTSNINNIKEIYSNDDLNCFLNNISAQKNLYTTHSGPSQAWIEHAYPLQQVTILLQGT
                     RHSEREDIISQLKIILSRLKNGENTGFEHDDDFGYSFQYDAAAVGKSSFFDEAAGRK"
     gene            1873..2408
                     /locus_tag="ACHURS_002436"
                     /pseudo
     CDS             1873..2408
                     /locus_tag="ACHURS_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542050.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
     gene            2424..2828
                     /locus_tag="ACHURS_002437"
     CDS             2424..2828
                     /locus_tag="ACHURS_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001576899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFR1238142.1"
                     /translation="MALSPKEKKELQQRFEKQLLPDLDMSFELNGEVYKFNFDKNESG
                     DYIDSFTQAVWGGYQDAEEWEKYGKHQETELPECPEPVEGWCDCDKCKSMIFHKEKNI
                     YVCLSCHKQTEPFLPIDWNSNDYDWLPYEDEY"
     gene            2864..3112
                     /locus_tag="ACHURS_002438"
     CDS             2864..3112
                     /locus_tag="ACHURS_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1238143.1"
                     /translation="MHSSFGLPYPAGHWFYSLQDLLDNPVFMVSFFVFWVATGQFLLT
                     TAHRKFNISETVEMVIIALLMILMTLSFYLCAILKASF"
     gene            3130..3459
                     /locus_tag="ACHURS_002439"
     CDS             3130..3459
                     /locus_tag="ACHURS_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001330833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1238144.1"
                     /translation="MVTIIIGVSRVIDYKMGGVSVDIPPFPEWNSGHYIEIPGEGIVF
                     TGEKDDDGKRCYSGVIESHYSTDDWAFILSEYKNWLNDKERKFSYLFVLCNGELVYHE
                     VNKSELL"
     gene            3486..4073
                     /locus_tag="ACHURS_002440"
     CDS             3486..4073
                     /locus_tag="ACHURS_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6012 family protein"
                     /protein_id="HFR1238145.1"
                     /translation="MYLHIVPKLFHRMANKCTLKSISIPELDLIIDGESLSVGRPWPN
                     KCLWVGMRKSRKAVNGLLLHTDKKLRWFTTRYTWDIEDMGLIHHQVNTYIEDNEFDMV
                     SQEILLNGSFDKWSDRVHSAYEGKPPAIIQPKMESFLKRPGEDSHDMWEEFEWGNFLI
                     SREESILLHTIQSERLTTDFSLFKRQPSIESALVI"
     gene            4085..6241
                     /locus_tag="ACHURS_002441"
     CDS             4085..6241
                     /locus_tag="ACHURS_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFR1238146.1"
                     /translation="MKLFIAEKPAVANDIVKALGGNFTRHDGWFESDNTIVTNCFGHI
                     IESQPPENYNPEYKEWKIETLPLRLYPVKYQPVESAEKQVKTIIELIRRADVTEIIHA
                     GDPDDEGQLLVDEVLEYAGNTKPVKRVLINDNTLPAVKKALANPKNNRDFRGLYLKAL
                     ARSVADAIYGLSMTRAYTIPAKTKGYKGVLSVGRVQTPVLGLIVNRTRANKNHKSSFY
                     YTMTGHFQRGADVIRANWKPGEFAPLTDRKLLDKTWANGTATSLAGKPATVEAAATDD
                     KKTAAPLPFNLVRLQQYMNKKFKMTAQKTLDITQQLREKYKAITYNRSDCSYLSDEQF
                     SEAPQIIDALKSVFDQPMDIDTTRKSKAFNSAKVTAHTAIIPTVSVPDVNALSTDERN
                     VYLAIAQHYLVQFMPEKAYQEVSVAIQCGDESFYARARKTTDSGFEAFLGVENAGDDE
                     AEVENDDSAFDLLCKIRTGETVTTKEVVVNEKKTTPPPLFTEATLLAALVRVADFVTD
                     PVIKKLLKDKDRDKKDEHGGIGTPATRASILETLKKRNYITLEKGKLIPTDTGYALID
                     ALPDIAVNPDMTALWAEKQTLIENGEMTIEQFVDELYNDLIPMISNANSAEIKVSPSA
                     PSGQSQRLSSPCPSCGKQIVIRPKSYSCTGCEFKIWNEFSGKKITQAQAEKLIKSGKT
                     DLIKGFKKKSGGTYDAVLVLEDKKTGKLGFPARAKK"
     gene            6716..7054
                     /locus_tag="ACHURS_002442"
     CDS             6716..7054
                     /locus_tag="ACHURS_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1238147.1"
                     /translation="MKFIILKKDKDSTIARNDSVFPVNNFFVVLTTVSSVANSFGIVS
                     SLAKSIGISFPTGNVIETCFFGMFSLLLAAFSFVSTMGLANAVPPIKNVFPLLFFVLV
                     LITNYNIYFR"
     gene            7173..7379
                     /gene="hha"
                     /locus_tag="ACHURS_002443"
     CDS             7173..7379
                     /gene="hha"
                     /locus_tag="ACHURS_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFR1238148.1"
                     /translation="MKTKQDWLFQLRRCSTLDTLEKIIEKNQNCLPPSELESFNAAAD
                     HRLAELTMNKLYDKVPPSVWKYVY"
     gene            7525..7968
                     /locus_tag="ACHURS_002444"
     CDS             7525..7968
                     /locus_tag="ACHURS_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000465834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1238149.1"
                     /translation="MFFLYETYNFFYYLIKLIVIQPQYICVYMIFFFFNAGIAYSITN
                     DIEDQVCRWLLFVSMLHALMIPPAVIMPLIMPPKEILQETEKRQELHESIPKTCKLKA
                     LDAQQGGLFGVDKDEWVFPDNKSFYLPEKCRPENRITELAMMKEG"
     gene            8213..8653
                     /locus_tag="ACHURS_002445"
     CDS             8213..8653
                     /locus_tag="ACHURS_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000666090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1238150.1"
                     /translation="MKEYEKTALIYSLKESNTLVLRIILISILVVGVISGAIYLYNLA
                     ISLFDSNPTSVQFTFCGVGCFIALLLQWISNVDANYRERIGEQPSTIKNIVQYLFAAI
                     ITISFITMALTAILVLKDSSNIWLNITFIALLLSIFVAIISVWG"
     gene            complement(8696..12907)
                     /locus_tag="ACHURS_002446"
     CDS             complement(8696..12907)
                     /locus_tag="ACHURS_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD7 domain-containing protein"
                     /protein_id="HFR1238151.1"
                     /translation="MIVRYGGGNDGIVDYLINGRKAERQYTRDELDHRVVLDGDLQTT
                     DKIIDSIENKSQERYLHITLSFHESHVSNEVLKAIVDDYKKLLMNAYHPDEYSFYAEA
                     HLPKIRHIQDNSTGELVERKPHIHIVIPKVNLITEKFLNPVGDVTKGHTIEQLDAIQE
                     FINNKYNLDSPKDYPRKDADYGKIISRVKGDLYKEHHSELKGELLSRIENEKIENYSV
                     FKDIVAEYGELRIRNAGKTNEYLAVKLPGDKKYTNLKSPLFRQNYIETRTLTLEKPTH
                     KEIEKRLNTWLNKTSQEIKHIFNQAEKTRELYKTLSPLQQIDFLQERIKEYDSREKLN
                     ERNSQQTSGREGGYKSCPKKFARIRQSEATVGLSRMPQRGMVYGINGFTRPDSVSVLS
                     DISQRDLAEQLPQREHPGQDVRRDYDRQFTESGIKSLERSSFLCETMFQTLNEAAEKN
                     EITTMAEIRRNIDPVRFLSSAAERFNIIPAQHKIRTAKDGSPRFSVGNRNMNASDFLT
                     KHINLAWKDAKSFLLEVYSQQLENTPYTRYPTYRRLTYHEARERLNSLNLSEKTLRNT
                     IRFERGKLYNDLREMRRELKLIPREQRDIAVGVIVYKKLTTLERLSELDTEGRHIIRQ
                     YHADWHKDKDEMKALERLKSYLNFDEINAISADEPELSLQKAVDSQRRLEEAKKVNSK
                     LKDLVMDKQDSRIVYRDQESEKPVFTDKGNFVVAGKNPSKEEIGIMLEYSREKFGGVL
                     KLTGSEDFKKMCAEVAAEQDMKIILRPEQYQQMMLELKAELQGNKFEQVETQENSQES
                     ESRIEKGDALKEQATEQAQATEQTQATEQAQATEQTQATEQAQATEQAQATEQAQATS
                     SYDPGVITRANTLDSQMLSKGTNGEFGYLKSLDSDENEIWEVLGHVPGDSDDIFSVAS
                     FDNENDAKEFCNIVNELGIDRTQALIQEQLTTQHDQATAQVHNKQEIYCINFSRFHDL
                     NEGIVFHSKDAAIQCYEESKSSAIEKYESNYLNGDGFDTVVLMSKTVSTDELSSYPEG
                     ALVTFDRPFEIIANSYEEYRTPVYAVSFSKDEFSEDIKTFESLHDASEYKNKMLQEHG
                     LNQDDILITPVTREEIAFKGIKDAVNDANMAVMEQAGDSSRESPEEILASISANEHMI
                     SGLENFLVKDRSQFSSCNGDIVVEAEITRGEGGLYRLAVAGKHGLERGDAVARVDVTE
                     QQFAAITGKTPSEVLTGDQTSARVPVITGIHFSTRAIENVNKLEQQKDYVYFSTHEGL
                     NAEIKDFSSLKDAIEWGRVECELHDLNKRDTVIYRVESEHISQGIDAVMKNAERVERH
                     EIEKAQGRDCTPEDGKILEAIDRFEDKFRGEGLKFEREKAESDLLNHGFTREMAEDAL
                     GKQFVQAREDHQESQQQRDDSQDMH"
     gene            complement(12904..13245)
                     /gene="mobC"
                     /locus_tag="ACHURS_002447"
     CDS             complement(12904..13245)
                     /gene="mobC"
                     /locus_tag="ACHURS_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFR1238152.1"
                     /translation="MKKKTNKNVHVTFRLTEEEYAPFDRAIKELNISKSEFFRLLTIG
                     KINTYASDKRNIPEYKRCLSQLSWAGNNINQIAHRLNSDHLKGIISESLYKKVLNGLI
                     GIRDRLQEIAK"
     gene            13575..14054
                     /locus_tag="ACHURS_002448"
     CDS             13575..14054
                     /locus_tag="ACHURS_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1238153.1"
                     /translation="MNNGENKLLGSLLAQKVKRSKTGRIRERFAEIEEAQQQGIRNID
                     IVNALNDEGFDLTLKTFENILHRIRKERAEKKDVSHLLSNKEKTYQKAITIEDKNRKT
                     KQDNDILNAYLPVCFNNAKIAQQAIDNNVSIETIKSWNCANFVQVSNTLGNYIRNKR"
     gene            14060..14761
                     /gene="stbB"
                     /locus_tag="ACHURS_002449"
     CDS             14060..14761
                     /gene="stbB"
                     /locus_tag="ACHURS_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StbB family protein"
                     /protein_id="HFR1238154.1"
                     /translation="MYLKLAIVNNSGNVGKSTIGQTLLKPRLEGSEIIKVETLNTDGT
                     NDEKLAAKEFDEIIKRIDDSECTIIDVGSSNIEQFLVQMNEYQGSHDLIDYFIVPVVR
                     EAKQQIDSIQTITTLMAMGVEQDRFKVIFNLAEKDTPINKQYSVFLADDVCKEIVGEN
                     PVVVYHNNIFNILNKSGLQYDDVYNDNRDFRSLIRSASSKEERQDLSNLRAVKMLMNG
                     FNSDLDVAFKNLNLV"
     gene            14808..15407
                     /locus_tag="ACHURS_002450"
     CDS             14808..15407
                     /locus_tag="ACHURS_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1238155.1"
                     /translation="MSRSISEEFKDQKINDLLEDYIALEKQIEKTANLIINAIDETPA
                     RLDAVLSDKVNKIIQSSIDINKDINIAKSDIRQISNECQSEITKQAINTFSGIKQNFD
                     QIASDHKKQLEILTKSAKPFSIKTAIAICALCTLCLSAAFSGATWYVAQHEKEASLRF
                     YARAFLDMKKITEESMRKLPKSDQQNIKLKLDEIDSRKP"
     gene            15427..15549
                     /locus_tag="ACHURS_002451"
                     /pseudo
     CDS             15427..15549
                     /locus_tag="ACHURS_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15881..16144
                     /locus_tag="ACHURS_002452"
     CDS             15881..16144
                     /locus_tag="ACHURS_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1238156.1"
                     /translation="MATLTKKERAWLNELQDVLDRCPSPKKIGFYTIGDKSIYLYDLR
                     RMDEIMEALDNRSSMDWCVAVHDMNAGFDEKILFPSSVESTAG"
     gene            16155..16475
                     /locus_tag="ACHURS_002453"
     CDS             16155..16475
                     /locus_tag="ACHURS_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1238157.1"
                     /translation="MTTFTDKELIKEIKERIGSLDVRDNIERRAYEIALASLEAEAPN
                     LPGGFTIEEAKELHENLVKSHVSKALSGEKMKKENRDADLRWIHGVIVQAAWFVKASL
                     GQNA"
     gene            complement(16606..17043)
                     /locus_tag="ACHURS_002454"
     CDS             complement(16606..17043)
                     /locus_tag="ACHURS_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFR1238158.1"
                     /translation="MFFSVGVETPKDDHTAYGITVPVFDCFDFGCVSAADSQAEIPAM
                     AREAILAIVEEMVISGAHSVDDIHDEGCLTYSANPNYNHCDSWFVIDVDLSEIEGKQQ
                     RINISLPDVLIRRIDGYVRESGGVYKDRSHFLAQAARHELAYK"
     gene            complement(17068..17250)
                     /locus_tag="ACHURS_002455"
     CDS             complement(17068..17250)
                     /locus_tag="ACHURS_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFR1238159.1"
                     /translation="MKSADLLKELIAAGCELKRHKASSHQIWWSPITGKTFPVPHPKK
                     DLPLGTVRSIKKMAGI"
     gene            17547..17990
                     /locus_tag="ACHURS_002456"
     CDS             17547..17990
                     /locus_tag="ACHURS_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1238160.1"
                     /translation="MTQLIPEKILEIIDDHDRAEKKQRNKIGFIYLCLCLAIIGVAAY
                     TFISTFSINVVTIIIMLFGVFMIVIFKSYLDSWSGIILSSDHILSILDKTKDYPEIKR
                     IVINRLLSGSILTGKDEEYIYSQLEKAQQSNEREKRLQAIKEYTS"
     gene            complement(18378..18656)
                     /locus_tag="ACHURS_002457"
     CDS             complement(18378..18656)
                     /locus_tag="ACHURS_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000545933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CbiX"
                     /protein_id="HFR1238161.1"
                     /translation="MHFTTFLKKHFDIEKVVGTSDSGNDTESIYVYEKGNDCEPLFIL
                     HESWLNAEIKKCGVWTIGNIYSTLEHGKEYSEQELIKMIKEGKVISKY"
BASE COUNT         5715 a         3871 c         3653 g         5423 t
ORIGIN      
        1 tgcatttcga agcaagaatc tctaccttcc cccctttttt gtctggaggg gatatgtttc
       61 agaaaatcaa aattcgcaaa atgacaatca gccgggcgct ggacaaatac ctgaagacag
      121 tatccatcca caaaaaaggc catctacagg agttttatcg cgtaaatgtg attaaacgtc
      181 accctattgc tgaacgctat atggatgaca ttaccactgt tgatattgcc aattatcggg
      241 atcaacggtt ggcgcaaatt aacccacgaa cggggcgtca aataaccgga aacaccgtcc
      301 gtcttgaact tgccctgttg tcatcattat tcaatatcgc ccgtgtggaa tggggaacat
      361 gccgcatgaa tcctgtcgaa ctggtcagaa aacccaaaat cagtagcggg cgcgatcgtc
      421 gcctgacttc aggagaagaa cgccgacttt cgcggtattt caaagaaaaa aatcaggctc
      481 tttacgtcat ttttcatctg gcgctggaaa cagcaatgcg acaaggtgaa atcttgtcat
      541 tgcgttggga acacgtcgat ctgcaacatg gggtagctca tcttccaaca accaaaaacg
      601 gtgctccacg cgatgttccg ctatccagga aagcccgtaa ttacctgcaa atgctgccga
      661 cacagcttaa cggtaatatt ttttcttata cctcttcggg attcaaaagt gcctggcgaa
      721 cagcacttca ggaactgaag atagaaaact tgcattttca tgacctccgt catgaggcta
      781 tcagccgatt tttcgaactt ggtacattga acgtcattga ggttgccgct atttcaggcc
      841 atcgctccct gaacatgttg aaacgataca cacaccttcg ggcttatcaa cttgtcagta
      901 aactcgatgc ccgacgcaag caaaccagca aaatcgctcc ctattttgtt ccctacccgg
      961 caacggtcga gaacagaaac gggcaagtcg ttgtgactct gagtgatttc gacctggaaa
     1021 caagcgcagc cacaaaagaa caggccatat ttcatgccag cgtgttgctg ctacgaacac
     1081 tggcacaagc cgcccagcga ggagaacgcg tccctactcc tggcgaactt cctacaaaca
     1141 ttgatgaacg ggtaatgatt tgtccattaa caaattagag gattcactta tgatagaaca
     1201 actacagagt gattttttat tctggatatt tatttacttc ctgatagtag gtttgcttat
     1261 cagaaacatt catcttacca gaaaaattaa atccatcgaa aaaaacaaaa tgttcccagc
     1321 agaggaatat gctgtcctgg acattgaaca gcatatgtcc aataaatata ttgggcggct
     1381 agtaacccgt tttatgaaga gaactaatgg tgcacctttg ttaattgtgt ttccagaata
     1441 cgataaagga gacaacaatg aagacaaaaa ttctccacca ttccaacttg taaaactaac
     1501 atccaacatc aacaatatta aagaaattta ttccaatgat gacttaaact gttttctgaa
     1561 taatatttcg gcacaaaaaa atttatatac aacgcactct ggacctagcc aggcatggat
     1621 tgaacatgca tatcctttgc aacaagtaac tattctctta caaggcactc gtcatagtga
     1681 aagggaagat ataatcagtc aacttaagat tattctttcc agactaaaaa atggagaaaa
     1741 tacaggtttt gagcatgacg atgattttgg atattcattt cagtatgatg ccgcagcagt
     1801 aggaaaatca tcatttttcg atgaagcggc aggaagaaaa taagaataac gctctatcca
     1861 ttgcacaggc aaattaacgt taatctttta tgaaaatctt attaacaata tttatttatc
     1921 tgcttaccgc ttttcagtct gcaaatgcag gtggaatacc ttatacattt aaccagaaaa
     1981 ttcttcaggg gaaagttatt cgcgttctcg atggcgacac catagaaatt aaaacactac
     2041 ccgcaaaaat tgttgtgtat gaagttccga tccgagtccg attgataaac atcgatgccc
     2101 ctgaaaagaa acaacctttt ggacgctggt caactagtca gcttaaaact ctggttgcag
     2161 gtaaacaggt gacagtctct tattcacata aagaccgcta cggacgcatt atcggtcatg
     2221 tgttcacgac gaacggcacg gatgccagcc gctttatggt tcagtctggc gctgcatggg
     2281 tatatgaacg ttacaacgtg gatgagtcat taccggctct gcaacgggaa gcgcaggaac
     2341 aaaaacgcgg tttgtgggcc gatgcaaatc cggttccacc gtgggaatgg cgatacaaac
     2401 ataactaact gatagaggtt tttatggctt tatcacccaa agaaaagaaa gagttacaac
     2461 aacgcttcga aaagcaatta ctacctgacc ttgatatgtc atttgaacta aatggcgaag
     2521 tatataaatt taattttgat aaaaatgagt caggtgacta tatcgatagt tttacacaag
     2581 cagtatgggg aggttatcag gatgccgagg aatgggaaaa atatggtaaa catcaggaga
     2641 cagaattacc tgaatgccct gaaccagtcg agggttggtg cgactgtgat aaatgtaagt
     2701 cgatgatttt tcataaagag aaaaatattt atgtttgcct ctcctgccat aaacaaacag
     2761 agccatttct tccaattgac tggaactcaa acgattacga ttggctacct tatgaagatg
     2821 aatactaatg ctgaatactt caaaatcata atgggggaaa gaaatgcatt cttcttttgg
     2881 tttgccatat cctgctggtc actggtttta ttctttacag gacttactcg acaatcctgt
     2941 atttatggtt tctttctttg ttttctgggt ggcaacgggt caatttttat taacaactgc
     3001 acacagaaaa tttaatatat ctgaaacagt tgagatggtt atcatcgctt tattaatgat
     3061 actaatgact ctttcttttt atctttgtgc aatattaaag gcttcttttt aaattagaga
     3121 ttaaaatcaa tggtgacgat aataattggt gtatcgagag ttattgatta taaaatgggg
     3181 ggagtgtcag ttgatattcc tccattccct gaatggaaca gtggtcatta tatagaaatt
     3241 ccaggagagg gtattgtatt cactggtgaa aaagatgatg acggcaaacg gtgttactct
     3301 ggtgtcatcg aatctcatta cagcaccgat gactgggcgt ttattctttc tgagtacaaa
     3361 aactggctga atgacaagga aagaaagttt tcttatctgt ttgtcttatg caatggtgaa
     3421 cttgtttacc atgaggtcaa caaatcagaa cttctttgac tacgataaag ataaaggaaa
     3481 tagatatgta cctacatatt gtccctaaac tttttcatcg catggcgaac aaatgcactc
     3541 taaaatccat ctccatacct gaacttgatt taataattga tggcgaatca ttgtctgtag
     3601 ggaggccgtg gcctaacaaa tgtttgtggg ttggaatgcg gaaaagcaga aaggcagtta
     3661 atggacttct tttgcataca gataaaaaac taagatggtt cactaccaga tatacctggg
     3721 atattgaaga tatgggatta attcatcatc aggttaatac ttatattgaa gataatgaat
     3781 ttgatatggt atcacaggaa attctcttaa atggttcttt tgataagtgg agtgacaggg
     3841 tgcattcagc ttatgaaggt aagcctccag caataatcca gcccaaaatg gaatcatttt
     3901 taaaaagacc aggtgaagac agtcatgata tgtgggagga gttcgagtgg ggtaacttcc
     3961 tcatatcaag agaagaaagc atcttgcttc acacgatcca gtctgaacga ttaaccaccg
     4021 atttctcttt attcaaaaga caaccatcta ttgaaagtgc attagttatt taaaggataa
     4081 taagatgaaa ctttttatcg cagaaaaacc cgcagtagca aatgatattg ttaaggcgct
     4141 tgggggcaat tttacccgtc atgatggctg gtttgaaagt gataatacta ttgtgactaa
     4201 ctgttttggt catattatcg aatcacagcc ccccgaaaac tataatcctg aatacaaaga
     4261 gtggaagatt gaaacactcc ctttgcgtct ttatcccgta aaataccagc ctgtagaaag
     4321 cgccgaaaaa caggttaaaa cgattattga gcttatcagg cgtgctgatg tgaccgaaat
     4381 tattcatgct ggcgatcctg atgacgaagg acagctactt gtcgatgagg tcctggaata
     4441 tgcaggcaac acaaaacccg ttaagcgcgt tctgatcaac gacaacaccc ttccggcagt
     4501 gaaaaaggcg ctggcaaatc ccaaaaataa ccgtgacttc agaggactct atctcaaggc
     4561 tctggcgcgt tctgtagccg acgcgatata cggtttatcc atgacgcgtg cttacacaat
     4621 cccggcaaaa actaaaggct ataaaggtgt tttgtctgtc ggacgggtcc agacacccgt
     4681 gcttggcctg attgtaaatc gcacccgtgc caataaaaat cataaatcca gcttctacta
     4741 caccatgacc ggacattttc aacgcggcgc tgatgttatc agggcgaact ggaaacctgg
     4801 tgagttcgca ccgctgacag accgcaaatt gcttgataaa acctgggcga atggaacagc
     4861 aacatcactt gcaggaaaac cagccacagt tgaagcggca gcaacagatg ataaaaaaac
     4921 ggctgcaccg ttgcccttta acctggtacg acttcagcag tacatgaaca aaaaattcaa
     4981 aatgactgct caaaaaacgc tggatatcac acagcagcta cgcgaaaaat acaaagcaat
     5041 tacttacaac cgttcagatt gttcatatct ttcagacgaa caattcagcg aagcaccaca
     5101 aataatcgat gccctgaagt cggtgtttga tcagccaatg gatattgata cgacacgtaa
     5161 aagcaaggca tttaacagcg caaaagtaac agcgcataca gccatcatcc caaccgttag
     5221 cgtaccggac gtaaacgcac tgagcaccga cgaacgcaac gtttacctgg caattgcaca
     5281 acactatctt gtccagttca tgcctgaaaa ggcatatcag gaagtatccg tcgccattca
     5341 gtgtggtgat gagtcgtttt atgcccgtgc caggaaaact acagacagcg gatttgaagc
     5401 gtttcttggt gtggaaaatg ccggggacga tgaagcagaa gtcgaaaatg atgattccgc
     5461 ttttgatctt ctctgtaaaa ttcgcacagg agaaacagtg acgacaaaag aagtggttgt
     5521 gaatgagaag aaaacaacgc caccgccatt attcaccgaa gccactttac ttgccgcgct
     5581 tgttcgtgtc gcagattttg tcactgatcc agtgattaaa aagttactga aggataaaga
     5641 tcgggataaa aaagacgaac atggcggtat tggtacgcca gctacgcggg cttccatatt
     5701 ggaaacactg aaaaaacgga actatatcac cctggaaaaa ggaaaactta ttccgacaga
     5761 taccgggtat gcgcttattg atgctctgcc ggatatagct gttaatccag atatgacggc
     5821 attatgggcg gaaaaacaga cactcattga aaacggagaa atgaccatcg aacagttcgt
     5881 tgacgaactg tacaatgatc tgataccgat gatcagtaat gcgaacagtg ctgaaataaa
     5941 agtttccccc agcgcgccgt ctggtcagtc acaacgcctg tcctctcctt gcccttcctg
     6001 tggtaaacag attgttatca ggccaaaaag ctattcctgt acaggatgtg aatttaaaat
     6061 ctggaatgag ttttctggca agaaaatcac ccaggcacag gccgaaaaac ttattaaatc
     6121 aggaaaaacc gatttgatta agggatttaa aaagaaaagc ggtggaacgt atgatgcggt
     6181 tcttgtcctt gaggataaga aaacagggaa gctgggtttt ccggcaaggg ctaagaagtg
     6241 aaagcaaagc agaaatggct tttcaggtca ggaattgctt cagttaatag ccaagtctgg
     6301 tcgtgggcga gcgccaaacc cgattgctga agcactggca ggaggtcgtt cccttgatga
     6361 gtttctcatc gagaaatgat tctacagccc tccttttctc tggagggcgt tatcctttga
     6421 aatatccgtt tattttgatg ataattctca tgtttcgttt ctcggcgtgg agagtacaga
     6481 ttttatggtg aatggtttgt tgcttttcgc tgttagtgta ttagtttttt tagtgataat
     6541 cctgatgtta catttgagaa cagccagtct tctttctgaa tgctctgaat atcgcacgaa
     6601 atgtgataat ctctatcatc tatgccagat gcaagagaaa gagttagaac atctacgtgc
     6661 actcattgat gcgcataaca tagattatta gacgttgctt tagggggatg gataaatgaa
     6721 atttattatt ttaaaaaagg ataaagatag cacaattgcc agaaacgact ctgttttccc
     6781 tgttaacaat ttctttgtcg ttttaactac cgtgtcttcc gtggctaact ctttcggaat
     6841 agtgtcttcc ctggctaaat ctatcggaat atcttttccc acgggcaatg ttattgagac
     6901 gtgttttttc ggtatgtttt cactattact ggctgctttc agttttgtaa gtacaatggg
     6961 gctggcgaac gcagtccccc ccattaaaaa tgtgtttccg cttttgtttt tcgttcttgt
     7021 cttaattact aattataata tttacttccg ataagaacgg gggcgcggca gcggcttttc
     7081 ttctgcccgt tctcccgtca ataaacccag cagtagaaat caaacatcgt tgtagctatt
     7141 atagggcaag gatgttcttc aggaattgta caatgaaaac aaaacaagac tggctctttc
     7201 agttaagacg ttgctcaaca ctcgacacac ttgaaaagat tattgaaaaa aaccaaaact
     7261 gcctcccccc ttctgagctt gagtcgttta atgccgccgc cgatcacagg cttgcggaac
     7321 tcacaatgaa taaactctac gataaagtcc ctccatccgt ctggaaatat gtatattagt
     7381 tttcatctat taatattaga tttttgatcg acacaaacat cagcaatccc tatacacctt
     7441 tcatagaatg aattgaaccc tgtatatagc ctgtctgaaa tggatatctg gcaggctatc
     7501 ttaatagtgt tataggtggt atgaatgttt ttcctctacg agacctacaa tttcttctac
     7561 tacctgataa agttaattgt cattcagcca caatatattt gtgtctatat gatattcttt
     7621 tttttcaatg cagggatagc atatagcatc accaatgata tcgaggatca agtatgtcga
     7681 tggcttttat tcgtaagtat gttacatgct ctgatgattc cccccgcagt tatcatgcca
     7741 cttatcatgc caccaaagga aatacttcag gaaacagaga aaaggcagga actacatgaa
     7801 tcaataccca aaacatgcaa attaaaggca ctggatgctc aacaaggtgg tttattcggc
     7861 gttgataaag atgaatgggt attccctgac aataaatcat tctatctgcc tgaaaagtgc
     7921 cgtccagaaa acaggattac ggaactagcc atgatgaaag aaggataaag atctatgaag
     7981 atattcaaat tgtttgttct ttgtatcccc cttctgttta gtcttttttt cgctggctac
     8041 ggcattttgt caaaaaatcc atttgaaaca ctaccgtgtc tgtccgccag tgctttttgc
     8101 ctttttctgt ttgacatact gaggcgatca tttatgaagt gaggtgtctt gatgttaacg
     8161 ttgaggttga gaaagttttg ttggttctta tacttataga ccggattaaa tcatgaaaga
     8221 atacgaaaaa acggctttaa tatattcctt aaaggaaagc aatacattag ttcttcggat
     8281 tatactaatc tctattttag ttgtcggggt gatttcaggt gcgatatact tatataactt
     8341 agcgatctcg ttgtttgatt caaacccaac gtctgtgcaa ttcacctttt gcggagtagg
     8401 ctgttttatt gctctattac ttcaatggat atctaatgtt gatgcaaatt acagagaaag
     8461 aattggggag caaccaagta caataaaaaa cattgtacaa tatttatttg cagccattat
     8521 taccattagt ttcattacaa tggcattaac agccatatta gtcttaaaag actcgtccaa
     8581 catatggctc aacattactt ttattgcatt attactgtcc atttttgtag ctattatttc
     8641 tgtttggggt tgactttttt tcttaaaaag cccgaaaacc gggctttttt cttaattagt
     8701 gcatgtcctg tgaatcgtca cgctgctgct gcgattcctg atgatcttct ctggcctgga
     8761 caaattgttt tccaagagca tcttctgcca tttcacgagt gaatccgtga ttaagcaaat
     8821 cggattcggc cttctccctt tcgaacttca gtccttcccc tctgaacttg tcctcaaagc
     8881 gatcaattgc ctcaagaatc ttgccgtctt ccggcgtgca atcccgtccc tgtgctttct
     8941 caatttcatg acgctccacg cgttcagcat ttttcattac agcgtcaatc ccctgagaaa
     9001 tatgttcaga ctcgacgcga taaatgacag tatcgcgctt attgagatca tgtagctcgc
     9061 attcaacccg gccccactca atagcatcct tgagagagct gaaatctttt atttcagcat
     9121 tcagaccttc atgtgttgag aaatagacat aatccttctg ttgttccagt ttattcacat
     9181 tttcaatagc ccttgtactg aaatggatac ctgtaattac cggaacacga gccgatgttt
     9241 ggtcccccgt taaaacttca gaaggtgttt ttccagtgat cgcggcaaat tgctgctccg
     9301 taacatccac tctggcaact gcatcaccac gctctaagcc atgcttacca gcgaccgcaa
     9361 ggcgataaag accaccttcc cccctggtaa tttccgcctc cacaacgata tccccattgc
     9421 aggaactgaa ctgagaacgg tctttaacca ggaagttttc aagcccggag atcatatgct
     9481 cgttagctga aatactggcg agtatctctt ctggtgattc tctggacgaa tcccccgcct
     9541 gttccatcac tgccatatta gcgtcattaa cagcgtcctt aattccttta aaggcgattt
     9601 cttctcttgt tacaggtgtg attaagatat catcctggtt aagcccgtgc tcctgaagca
     9661 tcttattttt atattcagat gcgtcgtgca atgactcaaa agtcttaata tcttcactaa
     9721 actcatcctt cgaaaaagaa accgcataca ctggggtacg gtattcttca tatgaattgg
     9781 caattatttc aaatggccta tcaaacgtta ctaatgcccc ctctggataa gaggataatt
     9841 catcggttga gacagtttta gacataagca caacagtatc aaaaccatca ccatttaaat
     9901 aattgctttc atatttttct attgcagaag atttgctttc ctcataacac tgaatagccg
     9961 catctttaga atgaaagacg ataccttcat tcaaatcatg gaagcgacta aagtttatac
    10021 aataaatttc ctgtttatta tgcacctgcg cagtagcctg atcgtgttga gtagtaagct
    10081 gctcctgaat cagagcctgt gttctgtcaa ttcctaattc attaacaata ttgcaaaatt
    10141 cttttgcatc gttttcatta tcaaaactgg caacactaaa gatatcatcg ctgtctccag
    10201 gtacatgccc cagcacttcc caaatctcat tttcatcgct atccagagat ttcaaatagc
    10261 caaattcacc atttgtgcct ttactaagca tctgtgaatc cagggtattt gccctggtaa
    10321 taacaccagg atcatatgaa gatgtggcct gtgcttgctc tgtggcctgt gcttgctctg
    10381 tggcctgcgc ctgctctgtg gcctgcgtct gctctgtggc ctgcgcctgc tctgtggcct
    10441 gagtctgttc tgtggcctgt gcctgttcgg tggcctgttc cttcaatgca tcacctttct
    10501 ctattctgga ttcggactcc tggctatttt cctgtgtttc cacctgctca aacttattac
    10561 cctgaagttc tgcttttaat tccagcatca tctgttgata ctgctccggg cgtaaaataa
    10621 tcttcatgtc ctgctctgcg gcaacttcag cgcacatttt tttgaaatct tcagagccgg
    10681 taagtttgag tacaccacca aacttttccc tggaatactc aagcattatt ccgatctctt
    10741 cttttgaggg atttttaccc gcaacgacaa agttcccttt gtcggtaaag acaggctttt
    10801 ccgattcctg atcgcgataa acaatcctgg aatcctgctt atccatcaca agatctttta
    10861 gtttggagtt aacctttttc gcctcttcta agcggcgctg ggaatccacc gctttctgaa
    10921 gcgaaagttc gggttcgtca gcagaaatgg cgttgatttc atcgaagttg agatagcttt
    10981 tgagacgttc aagggctttc atttcatctt tatccttatg ccagtcagca tgatattgac
    11041 gaataatgtg tcgcccctct gtgtcgagtt cagaaagacg ctcaagtgtg gttagttttt
    11101 tgtaaacaat cacacccacg gcaatatcac gctgttcacg tggtattaac ttcaactcgc
    11161 gtctcatctc tcgcagatca ttatacagct tgccacgctc aaacctgata gtatttcgta
    11221 atgttttttc cgataaattc agtgagttaa gacgctcacg ggcttcatga tacgtaaggc
    11281 gtctgtaggt tggatagcgc gtatatggcg tgttttctaa ctgctgtgaa taaacttcaa
    11341 gcagaaagga tttcgcatct ttccaggcaa gattaatatg ctttgtcaga aaatcagatg
    11401 cgttcatatt acgattcccc acagaaaacc ggggggaacc gtcctttgct gttctgattt
    11461 tatgctgcgc tggaataata ttaaaacgct cggcggcaga agacagaaac ctgacaggat
    11521 caatatttct gcgaatttct gccatcgttg taatttcgtt tttctctgcc gcctcattca
    11581 gagtctgaaa catcgtttca cacaaaaaag aagagcgttc aagacttttt attcctgact
    11641 ctgtaaattg tctatcataa tcccgtcgca catcctgacc aggatgttct ctttgcggta
    11701 attgctctgc cagatcacgc tgcgaaatat ctgacaatac actgacagag tcgggtcttg
    11761 tgaatccatt gattccgtaa accataccgc gttggggcat acgtgacaat ccgactgttg
    11821 cttcagattg acggattctg gcaaactttt taggacacga cttgtaacct ccctctcgtc
    11881 ctgacgtttg ctgagaattt ctttcgttaa gtttttctct tgagtcatat tcttttatcc
    11941 tctcttgcag gaaatcaatt tgttgtaaag ggcttaatgt cttgtaaagt tcccttgttt
    12001 tctccgcctg gttaaaaata tgcttaatct cttggcttgt tttatttaac caggtattaa
    12061 gtctcttttc tatctctttg tgtgttggtt tctcaagtgt taatgtcctt gtctcaatgt
    12121 aattctgacg aaaaagagga ctttttaagt ttgtgtattt tttatctccc ggcaatttta
    12181 cagccagata ctcattggtt ttaccagcat ttcttatacg aagttcacca tactctgcaa
    12241 caatatcttt gaatacagag taattttcaa ttttctcgtt ctctatacgc gaaagcaact
    12301 cacctttcaa ttcagagtga tgctctttgt aaagatctcc cttaactcta cttataattt
    12361 ttccgtaatc cgcatcttta cgcggatagt ctttagggct atcaagatta tatttattat
    12421 taataaattc ctgtatagca tcaagctgtt caattgtatg ccctttggta acatctccca
    12481 ctggattcag gaacttttca gtaatcaaat ttactttagg aattacaata tgaatatgtg
    12541 gttttctttc aacaagttct ccggtgctat tatcctggat atgacgaatt ttaggcaaat
    12601 gagcttccgc atagaaagag tattcatcag gatgataagc attcatcaat aactttttat
    12661 aatcatcaac aatagccttt aacacttcat tcgacacatg actttcatga aaagacagag
    12721 taatgtgcaa ataacgttcc tgacttttgt tttcaataga atcaataatc ttatctgtag
    12781 tctgtaaatc accatcaaga acgacacgat gatcaagttc atcacgcgtg tactggcgtt
    12841 ctgctttgcg accatttatc agataatcaa cgataccatc attaccacca ccgtatctga
    12901 caatcattta gctatctcct gaaggcgatc acgaataccg attagtccat ttaaaacctt
    12961 tttataaagc gattctgata taataccttt taaatgatct gaatttaatc ggtgcgctat
    13021 ttgatttatg ttgtttcctg cccaactcaa ctgagaaaga caacgtttgt attctgggat
    13081 gttacgttta tcagatgcat atgtgtttat tttaccaata gtaagcagcc tgaaaaattc
    13141 tgacttacta atattaagct cttttatagc cctatcgaac ggagcatatt cttcttcggt
    13201 aagtctgaat gttacatgaa cgtttttgtt ggtttttttc ttcatatgtc ctcaaggttc
    13261 gtagaaccgc ataagaacgg ctgcgtcgtt cggggtttca aaggggcatc cccctttggc
    13321 acacgattgt aacatgaccg gaacggtctt gtgtacaatc ggtatcgtgc ctgtgtttga
    13381 gaaaattatt tgattccata gcattcacac atcaaatgca caccataaac atatctaata
    13441 catagcaaaa acacaccata aaaaccaatg acatagcaca tacatagcaa acacatagca
    13501 tatacatagc aaaaattgct tgacgttcat gatagaattg ctcatcatga tttcactgtc
    13561 ctggagactg acaaatgaac aatggagaaa ataaactact tggttcgctc ttagctcaga
    13621 aggtcaagcg aagtaaaact ggaagaataa gagagcgttt tgctgaaata gaagaagcgc
    13681 agcaacaagg cataagaaac attgatattg ttaatgctct taatgatgaa ggttttgatc
    13741 taacgttaaa gacgtttgaa aacatattgc atcgcatacg aaaagaaagg gccgagaaaa
    13801 aagatgtttc tcatcttctt tcaaataaag agaaaacgta tcaaaaagca ataaccattg
    13861 aggacaaaaa tagaaaaacg aagcaagata atgatatact taatgcatat ttgccagttt
    13921 gcttcaacaa tgcaaagata gctcaacaag ctatagataa caatgtatct atagaaacaa
    13981 taaaatcttg gaattgtgct aactttgtac aagttagcaa tactcttgga aattatatac
    14041 gaaataaaag gtaagaaaaa tgtacttaaa actagcaatt gtaaataata gcggaaacgt
    14101 agggaaatcg accattggtc aaactttatt gaaacctcgt ctggaagggt cagaaattat
    14161 aaaagtagaa acattaaaca ctgatggtac aaatgacgag aaattagcag caaaggagtt
    14221 tgacgaaatt atcaaacgta ttgatgatag tgaatgcacc atcattgacg taggctcatc
    14281 aaatattgag caattccttg tacagatgaa tgaatatcag ggaagtcatg accttattga
    14341 ttatttcatc gttcctgttg tgcgagaagc aaaacaacaa atagactcaa ttcaaaccat
    14401 tacaacacta atggctatgg gtgttgaaca ggatcgtttt aaagtaattt tcaatcttgc
    14461 tgaaaaggac acccctatta acaaacaata ttcagttttc ttagctgacg acgtatgtaa
    14521 agaaattgta ggtgaaaatc ctgttgttgt ttaccataac aacattttca atattttaaa
    14581 taaaagcggc ctacagtatg atgatgtcta taatgacaat cgtgattttc gttcattaat
    14641 tcgttcagcg tcttcaaagg aagagcggca agatttgtca aatttaagag cagtaaaaat
    14701 gttgatgaat ggattcaaca gcgatcttga tgttgccttt aaaaacctca atctcgtctg
    14761 atacaatatg gccgggatgt acccggtcat aaaattggag aaaaacaatg tctcgtagta
    14821 tctctgaaga gtttaaagat caaaaaataa acgatcttct tgaagattat attgctttag
    14881 aaaaacagat cgaaaaaaca gcaaacttaa ttatcaacgc tatagacgag acacctgctc
    14941 gtctggatgc cgttctgtca gacaaagtaa ataaaataat acaaagcagc attgatatta
    15001 ataaagatat taatattgca aaaagcgata taagacaaat atctaatgag tgtcagagcg
    15061 agataaccaa acaagcaata aatacttttt ctggaatcaa acaaaatttt gatcaaattg
    15121 cttccgacca taaaaaacaa ctagagatac taactaaatc agcaaaacca ttcagtatca
    15181 aaacagccat tgcaatttgt gccctgtgca cattatgcct gtctgcggca ttttctggcg
    15241 caacctggta tgttgcacaa catgaaaaag aggcaagtct gcgcttctat gcaagagcat
    15301 ttcttgatat gaaaaaaata actgaagaga gcatgagaaa attaccaaaa tcagaccagc
    15361 aaaatataaa actcaaacta gacgaaattg attcaagaaa accataaata tgaaaaggag
    15421 tccctaatga ccacttttac cgacgaggac aaagaactaa ttaaagaaat cagagagcga
    15481 attggcagcc tggacgtgcg agacaatatt gagcgtcggg cttatgaaat tgctctggca
    15541 tcgctggaag cagaggcagt tatgttctgt atatcaggac aaaatgtaga ttcagaagaa
    15601 catgtatcaa ccagcaaagc ggttgttgat gcctgggttg aagaatggaa tcaggttgac
    15661 ggaagtcctg gcgaaccact gtacaaaact atgccactct actatcacgc tgccttgcca
    15721 gcgccggtag tgcctgaagg actggttaaa gcggtgcgtt tttatgagca ggtaaaaaat
    15781 gagaatccgg cagtcgaaac cgtagcatgg aaagacgctg ttgactgggt tctcgaagag
    15841 gcttgtcagg ctgtaaacat tggcatcaaa ggagagtgat atggcaactt taacaaaaaa
    15901 agaacgggca tggttgaacg aattacagga cgttcttgat cgctgcccat caccgaaaaa
    15961 aattggtttt tacaccattg gcgataaaag catttacctg tatgacctgc gccgcatgga
    16021 tgaaatcatg gaggctcttg ataatcgttc gtcgatggat tggtgtgttg ctgtccatga
    16081 tatgaatgcc ggatttgatg aaaagatttt attcccctca tcagttgaaa gcacagcagg
    16141 ataaggacta acacatgaca acgttcaccg acaaagaact gattaaagaa atcaaagagc
    16201 gtataggcag cctggacgtc cgagacaata ttgagcgtcg ggcttatgaa attgctctgg
    16261 catcactgga agcagaagcg ccaaatttgc ctggtggttt cacaattgaa gaagcaaaag
    16321 aattacacga aaatctggta aaaagtcacg taagcaaggc attaagtggc gaaaaaatga
    16381 aaaaagaaaa tcgtgatgcc gatttacgct ggattcatgg cgtaatagtt caggctgcgt
    16441 ggtttgtgaa agcatcacta gggcagaacg catagtgcgt acctgctttt ctcctggcaa
    16501 cggtggatct tccgcttcct cgtcgctcgc tcgctatgct cggtcgctgg actgcggcga
    16561 gcggttagct atcaaaaaaa atccccgcac aacgcgggga tagttttatt tatatgcaag
    16621 ttcatgacgt gccgcctgag ccagaaaatg gctacgatct ttgtatacgc caccactttc
    16681 tctcacatat ccatcaatac gacgaatcaa tacatcaggc aatgaaatat taatacgttg
    16741 ttgtttgccc tcaatttctg acaaatcaac atcaattaca aaccagctat cacagtggtt
    16801 ataatttggg ttggcggaat aagtcaaaca cccttcatca tgaatatcat caacagaatg
    16861 agcaccgctt attaccatct cttccacgat tgccaatatc gcttcacgcg ccattgcggg
    16921 tatttctgcc tgagaatcag cagcagaaac acatccaaaa tcaaagcaat caaaaaccgg
    16981 aacggtgata ccgtatgctg tatgatcatc tttcggtgtt tcgacaccaa cagaaaagaa
    17041 catggctacc tccaaagtcg gcggggatta aatccccgcc atttttttga tcgatctgac
    17101 agtacctagc ggcaagtcct ttttaggatg aggaacagga aaagtttttc ctgttattgg
    17161 cgaccaccat atctggtgac tgcttgcctt atgccgtttc aactcgcaac cagcagctat
    17221 cagttctttc aacaggtcag cagatttcat atgtcctccc taacctgagg ataattatac
    17281 acacacatac acacaaaggc aaattatttc attcgtatga cttatgaacc acactgtaaa
    17341 gacagctcaa tctttacagt gtaagtcatt tatccagttt tatcgctgtg tatgtgatac
    17401 atagaaaata atatagtatt ttactatatg atatctgatt gacactttta actgcacatc
    17461 tcatcattta catcgtaaag acaaacatag atttacaatg taaagctatg taattaaaca
    17521 ctccgtgtcc caaataagga aagaaaatga ctcaactaat cccagaaaag atactggaaa
    17581 tcatagatga tcatgatcgt gcagaaaaaa agcaacgcaa caaaatcggt ttcatttatt
    17641 tatgtctctg tttagcaata attggtgtgg ccgcgtatac ctttatatca acgtttagca
    17701 ttaatgtggt aacaatcatc ataatgcttt tcggtgtgtt tatgattgtg atttttaaga
    17761 gctatttaga ctcatggagt gggataattc ttagcagtga ccacatattg tccatactcg
    17821 ataaaaccaa agattaccca gaaattaaac gtatagttat taatcgtctg ctctccggct
    17881 caatactcac gggtaaagat gaagagtaca tatacagcca actggaaaag gcccaacaaa
    17941 gcaacgaacg tgaaaagaga ttgcaagcaa ttaaagagta tacatcgtaa tacaacgcat
    18001 tacgtcgaaa taccctggat catcgaagcc acatacccct aagcgcagcg caaccgccgg
    18061 aaaacgcacc acactgtaaa gacaataaaa tctttacaac gtaaagctaa ctatgtaata
    18121 caacgcgcta cacaataaga aagggagagc atgacactct ccccatatat gactcaaact
    18181 aaagcattat cccggttatg cgcccaccaa atgatttcgc taataatcca tgcttctatc
    18241 gcgggtgttt ttacaggttc cccatttaca atgcggtttg caagctctat catttcacat
    18301 actgacattt gagaatctcc aaagatgcat ctgtcatttg gtaacaggct gccccacagc
    18361 ctgttaccaa ccccaaatta atatttgctg attactttac cctccttaat catttttatt
    18421 agttcctgtt cagaatattc ttttccgtgt tccaatgtgg aatatatatt gccaatagtc
    18481 cataccccac actttttaat ttctgcattt agccaggatt catgcagaat aaacaacggt
    18541 tcgcaatcat ttcctttttc ataaacataa atagattcgg tatcatttcc agaatcagaa
    18601 gtaccaacga ctttttctat atcgaaatgt ttttttagaa aagttgtgaa atgcattttc
    18661 at
//
LOCUS       DAZPDU010000080        18515 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZPDU010000080 DAZPDU010000000
VERSION     DAZPDU010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18515
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.081"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            78..290
                     /gene="cspH"
                     /locus_tag="ACHURS_002458"
     CDS             78..290
                     /gene="cspH"
                     /locus_tag="ACHURS_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFR1238162.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            737..1042
                     /locus_tag="ACHURS_002459"
     CDS             737..1042
                     /locus_tag="ACHURS_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1238163.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAASEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            1149..1793
                     /gene="gfcB"
                     /locus_tag="ACHURS_002460"
     CDS             1149..1793
                     /gene="gfcB"
                     /locus_tag="ACHURS_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFR1238164.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            1790..2536
                     /locus_tag="ACHURS_002461"
     CDS             1790..2536
                     /locus_tag="ACHURS_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFR1238165.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            2536..4632
                     /locus_tag="ACHURS_002462"
     CDS             2536..4632
                     /locus_tag="ACHURS_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFR1238166.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSQLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            4678..5817
                     /locus_tag="ACHURS_002463"
     CDS             4678..5817
                     /locus_tag="ACHURS_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFR1238167.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            5805..6251
                     /gene="etp"
                     /locus_tag="ACHURS_002464"
                     /gene_synonym="yccY"
     CDS             5805..6251
                     /gene="etp"
                     /locus_tag="ACHURS_002464"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFR1238168.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     IKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            6271..8451
                     /gene="etk"
                     /locus_tag="ACHURS_002465"
     CDS             6271..8451
                     /gene="etk"
                     /locus_tag="ACHURS_002465"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFR1238169.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(8566..9864)
                     /gene="appA"
                     /locus_tag="ACHURS_002466"
     CDS             complement(8566..9864)
                     /gene="appA"
                     /locus_tag="ACHURS_002466"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFR1238170.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(9944..10036)
                     /gene="cbdX"
                     /locus_tag="ACHURS_002467"
     CDS             complement(9944..10036)
                     /gene="cbdX"
                     /locus_tag="ACHURS_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFR1238171.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(10049..11185)
                     /gene="appB"
                     /locus_tag="ACHURS_002468"
     CDS             complement(10049..11185)
                     /gene="appB"
                     /locus_tag="ACHURS_002468"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFR1238172.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            complement(11197..12741)
                     /gene="appC"
                     /locus_tag="ACHURS_002469"
     CDS             complement(11197..12741)
                     /gene="appC"
                     /locus_tag="ACHURS_002469"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFR1238173.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKTVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLPLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            complement(12875..13732)
                     /locus_tag="ACHURS_002470"
     CDS             complement(12875..13732)
                     /locus_tag="ACHURS_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFR1238174.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPISCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSQRQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRKRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            complement(13789..14127)
                     /gene="hyaE"
                     /locus_tag="ACHURS_002471"
     CDS             complement(13789..14127)
                     /gene="hyaE"
                     /locus_tag="ACHURS_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFR1238175.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSEFRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPGYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHP"
     gene            complement(14124..14711)
                     /gene="hyaD"
                     /locus_tag="ACHURS_002472"
     CDS             complement(14124..14711)
                     /gene="hyaD"
                     /locus_tag="ACHURS_002472"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFR1238176.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGRLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            complement(14708..15414)
                     /gene="hyaC"
                     /locus_tag="ACHURS_002473"
                     /pseudo
     CDS             complement(14708..15414)
                     /gene="hyaC"
                     /locus_tag="ACHURS_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
     gene            complement(15433..17226)
                     /gene="hyaB"
                     /locus_tag="ACHURS_002474"
     CDS             complement(15433..17226)
                     /gene="hyaB"
                     /locus_tag="ACHURS_002474"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFR1238177.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFNKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            complement(17223..18341)
                     /gene="hyaA"
                     /locus_tag="ACHURS_002475"
     CDS             complement(17223..18341)
                     /gene="hyaA"
                     /locus_tag="ACHURS_002475"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFR1238178.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAAWPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDCGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
BASE COUNT         4547 a         4915 c         4783 g         4270 t
ORIGIN      
        1 cggcggtctt aaatatcgct ggctttattt tattaacccc gtatagtgca gcgcagtatt
       61 ttcagtaagg aatttgtttg tcccgtaaaa tgacaggaat tgtcaaaacc tttgatcgca
      121 aaagcggcaa aggattcatt atcccctccg acggtcgtaa agaagttcag gtccatattt
      181 ccgcattcac tccccgcgac gcagaagtgc ttataccagg attacgtgtg gaattttgcc
      241 gcgtaaatgg cctgcgagga ccaacagcgg caaatgttta tctctcttaa tatgacgctg
      301 cttgtttaaa gctgactggt tttattggaa tagtgcaata gtcaacatca tacattataa
      361 catcagttac ttttccacaa agaaactaat ccatttaagc gaatatctct gcacctaata
      421 gttataaatg ccatttaaca gccagttata gtaccacttg aaactatatt accaacaata
      481 attaatagct ttcgtaacaa tttccttatc tttaatttgt attttgctaa gatttatctt
      541 atgctcacaa gtcgtatttc cagaggggat cgttaataat cttatatttc cctctgcgca
      601 cctttcctac ccgcagtatc agtatatagt gcaagcggta tgaggaaagg gagatttcac
      661 aaggatgtta tactcaaagt agacaatcct gatctcaagc gtacgtattg tccatgcgat
      721 gaataaagga aaagttatga aacacaagct ttctgcaatc cttatggcct tcatgttgac
      781 gacgccagct gcgtttgctg cctctgaagc aacaaacggg accgaggcaa cgacgggtac
      841 tactggcacc acaacgacca ctaccggcgc aaccacgact gctactacca ctggtggcgt
      901 ggctgctggc gctgtcggca cggcgacagt tgtcggtgtg gcaacggctg ttggtgtggc
      961 gacgttggca gttgttgcag caaatgacag tggcgatggc ggttctcata acacctcaac
     1021 caccacgtcc actacacgct aatactgctt taacgcgtcg aaattaaagt taaatgatac
     1081 ccggttgcga tattcgtacc gggctttaga gatttgcatt tttttaatta acctttcaag
     1141 gattaaaggt gcgccctctt attttatcga ttttcgcact atttcttgcg ggatgtacgc
     1201 acagccagca aagtatggtc gatacatttc gcgccagcct tttcgataat caggatatca
     1261 ccgtagcgga tcagcagatc caggcgttgc cttattccac gatgtattta cgccttaatg
     1321 aagggcaacg aatctttgtg gtactgggat atatagaaca agaacaaagc aaatggttat
     1381 cccaggataa cgccatgctg gttacccaca atggacgtct tttaaaaacc gtcaaactta
     1441 ataataatct gctggaagtg acaaattccg ggcaggaccc tctgcgtaac gcgctggcaa
     1501 taaaagatgg cagccgctgg acgcgcgata ttctctggag tgaagacaac cattttcgct
     1561 ctgcgaccct gagttctact ttttcctttg ctggattaga gacgctgaat attgcgggtc
     1621 gcaatgtgct gtgtaatgtc tggcaggaag aggtgacttc cacgcggcca gaaaaacagt
     1681 ggcaaaacac attctgggtc gattcggcta ctggccaggt tcgtcaaagt cggcaaatgt
     1741 taggcgcagg ggttattccc gtagaaatga cgtttcttaa acccgcacca tgaataaatt
     1801 acagtcgtat ttcattgcca gcgtacttta cgtaatgaca ccccatgcct ttgcgcaagg
     1861 aacggtgact atttatctgc ctggcgaaca acagacactt tccgttggcc ccatggaaaa
     1921 tgttgtccag ttggtgacac aaccgcaact gcgcgatcgc ctctggtggc ctggtgcctt
     1981 gctgaccgac tctgcggcga aagctaaagc gcttaaagac taccaacatg tcatggcaca
     2041 gcttgcttcc tgggaagcag aagctgacga cgatgtggcg gcaacgatta aatccgtgcg
     2101 ccagcagtta ctcaatctca atatcactgg tcgcttaccg gtcaaactgg accctgattt
     2161 tgtacgagtg gatgagaaca gcaatccccc gttggtcggg gactatacgc tctataccgt
     2221 acagcgtccg gtgactatta ctctgctggg tgcggtttct ggggccgggc aattgccctg
     2281 gcaggcaggc cgtagcgtta ccgattatct gcaagatcac ccccgccttg caggcgcgga
     2341 caagaataac gttatggtga ttacgcctga gggtgagact gtcgtcgccc ctgttgccct
     2401 gtggaataaa agacatgtgg aaccaccgcc tggcagccag ctttggttgg gcttttctgc
     2461 gcatgtactg cccgaaaaat atgctgattt aaatgaccag attgtgtctg ttttaacgca
     2521 acgtgttcct gactgatgaa gaagaattct tatcttttaa gctgcctggc cattgccgtc
     2581 tccagtgcct gtcatgctga agtattaacc tacccggatc cgctgggttc gtcgcaatca
     2641 gactttggcg gcacaggatt gttgcagatg ccaaatgcgc gcatcgcacc ggaaggtgaa
     2701 ttcagcgtca actaccggga taacgatcaa taccggttct actccacctc cgtggcgctg
     2761 ttcccatggc tggaaggcac cattcgttat acggatgtgc gcacacgcaa atatagccag
     2821 tgggaagatt tcagcggcga tcagtcatac aaagacaaat cattcgattt taaacttcgc
     2881 ctgtgggaag aaggttactg gctaccgcaa gtggcgtttg gtaaacgtga tattgctggt
     2941 acgggtctgt ttgacggtga gtatctggtg gccagcaagc aagcggggcc atttgatttc
     3001 accctcggga tggcatgggg ctacgccggt aatgcgggca atattaccaa cccgttttgc
     3061 cgggtgagcg ataaatattg tcatcgcgca gagtctcacg atgcgggcga tatcagcttt
     3121 agcgatatct ttcgtggccc ggcttccatc tttggcggca ttgagtatca aacgccgtgg
     3181 aatcccctgc gtctgaaact cgaatacgat ggcaacaatt accagaatga tttcgctggc
     3241 aaactgcctc aggcaagcca tttcaacgtc ggcgcagttt atcgcgctgc cagctgggca
     3301 gatctcaacc tgagttatga acgcggtaac acgttgatgt ttggcttcac gttacggacc
     3361 aatttcaacg atctgcgccc tgccctgcgc gatacgccaa aaccggcata tcaacctgcg
     3421 cctgaatctg aaggattgca gtacaccaca gtagcgaacc aacttaccgc gctgaagtac
     3481 aacgcaggtt ttgaagcacc ggaaattcag ctgcgcgata agacgctgta tatgtctggt
     3541 caacaataca aataccgtga ttctcgcgaa gcggtcgatc gtgccaaccg gattctggtg
     3601 aataacctgc cgcaaggcgt tgagaagatt agcgtgacgc aaaagcgcga gcatatggcg
     3661 atggtgacta ccgaaaccga cgtagccagc ctgcgcaaac agctggctgg tacagcgcct
     3721 ggtcaatcag agcaacttca acaacaacgt gttgaagcag aagatctttc tgcctttggt
     3781 cggggctacc gtattcgtga agatcgcttt agctactctt tcaacccaac actttcacag
     3841 tcgctgggcg gcccggaaga tttctatatg ttccagctgg ggctgatgtc cagtgcccgc
     3901 tactggttta ccgaccacct gctgcttgat ggcggtattt tcaccaatat ttacaacaac
     3961 tacgacaagt ttaagtcttc gcagttgccc gcggactcta ccctgccccg cgtgcgcacg
     4021 catatccgtg attacgttcg caatgacgtt tatctcaaca acttgcaggc gaactacttt
     4081 gccgacttag gcaatggttt ctatggccag gtgtatggcg gttatctgga aacgatgtac
     4141 gccggtgtcg gttccgagct gctttatcgc ccgctagatg ccagctgggc gctgggtgtg
     4201 gacgttaact acgtgaagca acgtgactgg gacaacatga tgcgcttcac cgattattcc
     4261 acgccaactg gtttcgtgac ggcttactgg aacccgccga cgctcaatgg cgtactgatg
     4321 aaacttagcg ttgggcaata tctggcaaaa gataaagggg caacgatcga cgtcgccaaa
     4381 cgctttgaca gcggcgtggc ggtaggggta tgggcggcaa tcagtaacgt atctaaagat
     4441 gactacggcg aaggcggctt tagtaaaggt ttttatatct cgattccgtt cgacttgatg
     4501 accattggac ctaaccgcaa ccgcgcggtg gtttcgtgga caccattgac gcgtgatggt
     4561 ggacaaatgc tgtcacgcaa ataccagctc tatccaatga cggcagagcg agaagtaccg
     4621 gttggacaat aacctgacgt aattcataac attacattga ctgataaata taaaccaatg
     4681 aaaaagaaca tttttaagtt ttcggtactg acattggctg ttctgtcttt gacagcatgt
     4741 acccttgttc ctggtcagaa tttatcgacc agtaacaagg atgtcattga actacctgac
     4801 aatcagtatg acctggacaa aatggtcaat atctatcccg tcacgccagg attgattgat
     4861 cagttgcgcg ccaaaccgat tatgtcgcag gctaacccgg agctggagca gcaaatcgca
     4921 aactatgaat atcgtatcgg gattggtgat gtgttgatgg ttacggtctg ggatcacccg
     4981 gaactaacca cccccgcagg gcaataccgt agcgccagtg ataccggcaa ctgggttaac
     5041 gccgatggtg cgattttcta cccctatatt ggtcgcctga aagtggccgg gaaaacactt
     5101 acccaggtac gcaacgagat tacagcgcgt ctcgattcag tcattgaaag cccgcaagtc
     5161 gatgtcagcg tcgcggcgtt ccgatcgcaa aaagcgtacg tcaccggcga agtcagcaaa
     5221 tcgggtcagc agccgattac caatattcct ctgaccatca tggatgcgat taacgccgcg
     5281 ggcggtctga ccgcagatgc agactggcgc aacgtggtgc tcacccagaa tggcgtaaag
     5341 accaaagtga acctctatgc gctgatgcag cgtggcgact tgcgccagaa caaactgctg
     5401 cacccgggcg atattttatt tattccgcgc aacgatgatc tgaaagtctt cgtgatgggt
     5461 gaagtgggta aacagagcac gctgaaaatg gaccgcagcg gtatgaccct ggcggaagcg
     5521 ctgggtaacg ctgagggtat gaatcaggat gtcgctgatg cgacggggat ttttgttatt
     5581 cgtgcaacgc aaaacaagca gaatggcaaa atcgccaata tttatcagct gaatgcgaag
     5641 gacgcatcgg caatgatact cggaacggaa ttccagcttg aaccttacga cattgtttat
     5701 gtcactaccg cgccgctggc tcgttggaac cgtgtgattt ctctgctggt accgacgatt
     5761 tccggggtgc atgacctgac agagacttca cggtggattc aaacatggcc caactaaaat
     5821 ttaactcaat cctggtggtt tgtaccggca acatttgccg ttcgcccatt ggcgagcgct
     5881 tgctgcgtaa acgtctgccg ggcgtaaaag tcaaatcggc aggtgttcat ggtctgataa
     5941 aacaccctgc agatgcgaca gcggcagatg ttgccgctaa tcatggcgta tctcttgaag
     6001 gacatgccgg acgtaagctc actgctgaga tggcacgaaa ttacgatctg attctggcaa
     6061 tggagtcgga acatattgct caggttaccg caattgctcc cgaggtccgc gggaagacaa
     6121 tgctgtttgg gcaatggctg gaacagaaag agatcccgga tccctatcgt aaaagtcagg
     6181 acgcatttga acatgtctac ggtatgttgg agcgcgccag tcaggaatgg gcgaagcgcc
     6241 tcagccgata atttgagttt ataaaatacg atgacaacta aaaatatgaa tacgccacca
     6301 ggcagcactc aggaaaatga gatcgatctg cttcgtctgg tcggcgagtt atgggatcac
     6361 cgtaagttta ttatcagcgt gaccgcgtta ttcacgctga tcgctgtcgc ttactcgctg
     6421 ttaagcacac caatttatca ggcagatact ctggtccagg ttgagcaaaa acagggcaac
     6481 gccattctca gcggcctgag cgatatgatc cctaactcat cgcccgagtc tgcaccggag
     6541 atccaactgc tgcaatcgcg catgattctc ggtaaaacca ttgctgaact gaatctgcgc
     6601 gacatagttg agcagaagta ttttccgatt gtgggtcgcg gctgggcgag attaaccaaa
     6661 gaaaaaccag gtgagctggc gatcagctgg atgcatattc cacaactgaa tggtcaggat
     6721 cagcaactga cactcacggt tggggaaaac ggccactata cactggaagg tgaagagttc
     6781 accgtcaatg gtatggtcgg ccagcgtctg gaaaaagatg gcgttgcgct gactatcgcg
     6841 gacattaagg ccaaaccagg aacacagttt gtcctgagcc agcgtaccga actggaagcg
     6901 attaacgcat tgcaggaaac ctttaccgtt agcgaacgca gtaaagaaag cgggatgctg
     6961 gaacttacca tgactggtga tgatccccag ttgattactc gtattctgaa cagcatcgct
     7021 aacaactatt tgcaacagaa tatcgctcgc caggcggcgc aggattcaca aagccttgaa
     7081 ttcttacagc gccagttacc tgaagtgcgc agcgagctgg accaggcgga agaaaaactc
     7141 aacgtttatc gccagcagcg cgattcggtt gaccttaacc tggaagccaa agccgttctt
     7201 gagcagattg tgaacgttga taatcaactc aatgagctga ccttccgcga ggcagagatc
     7261 tcccagctgt ataagaaaga tcacccaact tatcgtgcgc tgctggaaaa acgccagacg
     7321 ctggagcaag aacgcaaacg cctgaataag cgggtatcgg caatgccttc cacccaacag
     7381 gaagtgttgc gtttaagtcg tgacgtagaa gcgggccgtg cggtatatct gcaattactt
     7441 aaccgccagc aggagttgag tatttcgaaa tccagtgcca ttggtaacgt gcggattatc
     7501 gacccggcag tcactcagcc gcagccagtg aaaccgaaaa aagcgttgaa tgtggtgctt
     7561 ggttttattc ttggcctgtt tatttctgtg ggtgccgtgc tggcgcgtgc gatgttgcgt
     7621 cgtggtgtag aagccccgga acaactggaa gagcacggca tcagcgttta tgccactatc
     7681 ccaatgtccg agtggctgga taaacgcacc cgtctgcgta agaaaaattt attttctaat
     7741 cagcagcgcc atcgtactaa aaatatcccc ttcctggcgg tggataaccc ggcggattct
     7801 gctgtggaag ccgtacgtgc gctacgaacc agtctgcatt tcgctatgat ggagacggag
     7861 aataacattc tgatgatcac cggtgcgacg ccagacagtg gtaaaacgtt tgtcagttca
     7921 actctggcag cggtgatcgc ccagtccgat caaaaagtgt tatttattga tgccgactta
     7981 cgccgtggtt attcgcataa cctgtttacc gtgagtaatg aacatggctt gtcggaatat
     8041 ctggcaggta aagatgagct caacaaagtg atccagcatt ttggcaaagg aggctttgat
     8101 gtgattactc gcggtcaggt gccacctaac ccgtctgaac tgctgatgcg cgatcggatg
     8161 cgtcaattac tggaatgggc gaacgaccat tacgatctgg tgattgtcga tacgccgccg
     8221 atgctggcgg tgagtgatgc cgcggtcgtg gggcgttctg ttggcaccag cctgctggtt
     8281 gcgcgttttg gcttgaacac cgccaaagag gtgagtttgt caatgcagcg tctggaacag
     8341 gcaggcgtca atattaaagg cgctatcctc aatggtgtga ttaaacgcgc cagcaccgct
     8401 tacagttacg gctataacta ttacggttat agttactccg agaaagagta aaactgaaag
     8461 tggccggatg cggcgtgaac gccttttccg gtctacgcat tagacagttc ttcgtttgtc
     8521 atcagcctca gagcattcag gtaactgaat gctctttttt atgcattaca aactgcacgc
     8581 cggtatgcgt gcttcattca cgatttgcgt aaaacctgcc aacgaacaca tgccctgcgc
     8641 atttcgctct tcacatcctg ccagggtcag tttcacctct ccgggcggcg tatttaatga
     8701 cagcggcgtt ttatcacgca tctgctgtaa agtctggaag accagcgaaa cctgaatcca
     8761 ctggctgtta tcgcttagcc gacgccagcg ttcaaacacc agttcaccac ctggcggcgt
     8821 gttatccggc tgaccgggaa gcgtccagtt gagctccagt gcgccgccga gatttgccag
     8881 attagtatcg tgtccggcga taaacagcac tgaagtgggt aatgtcacac catacgcctg
     8941 tttttgcggt ggatggggcg tcaacgctgt cttgatcaaa tctaataacg gggtggcgcg
     9001 gctgcgggca acctctggcg tgcgttgtag caaataaaat tgcgcgttat gcaaacttag
     9061 caaggtgttc cactggtgtg aatcggtgat ccttccccac cccggctccg gcattccctg
     9121 tgcttgttgc aggagaaata tctccgtcag cattgatgcg aggcttaccg caccggttaa
     9181 tgagacattg tcggcgctca ccttgagttc cgatggtaat gcctgcgtta atgaacagct
     9241 ttcgtcctgt ttctcacgtt taaggcacaa gtttgattgc ggaaaattaa gcacccgttc
     9301 cagttcgcga aacgccgttt gccgatgccc ggtaaagtca gcaattgacc ctcctgccct
     9361 gctgaggatc gcgtcagtca cgttcgcgtt atccagttgg caaacgccag tttttagagg
     9421 attaaataac ggatcgggac tggacgtatc agcctgggta tgtacggtta ttgcacagtc
     9481 aggtgccagc ccggcggcga aggcttcgcc tgttttacgg gtacgctcgt cgacatcagc
     9541 aataatcgcg acctgaccag actgcgggca gccctttttc gccagcaatc cgtcggctac
     9601 cagacgctgg cgttggtaat gtccgagata ggcgattagc tcaccaccgc gcggtgtcag
     9661 ccaacccagt tttaccggcc aggttggcca tgcgtctggg gtgacatcct gcatcagttg
     9721 cgtggccttg gttggagcac gcacaccatg acgactgaca atcaccacac tttccagctt
     9781 cagctccggc tcactctgag cgaatgcaga ttgcggggtt aacggaatca gaagagataa
     9841 aaatgggatt aagatcgctt tcatcgatat gtttccgctt ttcatatctg acattattga
     9901 ttgcctgatg cgatgctaat gcgccgcatc aggcctacgt tcgttaactt ttcagacgcg
     9961 ggtccagcca taccaacaca agggtggaga gcgaacacat caacaaaatg ccgacgaacc
    10021 aaagtaaata ccacattgtt cctgctcctt agtacaactc gttttcgtta cggcggagag
    10081 tttctgttgt catgcgtccc cacattttgt agtagctcca gagagtgtag agcaacacaa
    10141 tgggcaaaaa tatcagcacg attaccaaca taatgctcag cgtcagctga ctggaagtgc
    10201 tgtcccacaa cgtcaggctg gagattggac tcacgcttga cggcatgaca aaggggaaca
    10261 gcgtgatgcc tgccgtgaaa atcacgccaa attgcatcaa tgacgccatt aaaaatcccc
    10321 aacccgggcg accacgataa atcgccatca ccgtcagcaa tgggcagaag aaccccagca
    10381 gcgggaagat ccacaaaacg ggcgattcga cgaaattatt catccaggca ccaggtagca
    10441 ctgccaccag tttcattaac ggattggaag gaccgttagc atcctgggcg agcagtacaa
    10501 agccatcaat accgacccac agccagtaac ccgccagcaa aaagcacagc atcaccaaca
    10561 gtgcagcgcg tttggtggcc agctgtgaac gcagatgaat cacaccaaca gttttcagtt
    10621 gtaaccagac gccaccttgc aaaatcacca tcccaaggct gagcaatccg cacaataaag
    10681 ggaatggcgt cagcagttgc cagaagctac cgagatactc cacgcgtaat tgcggtgtga
    10741 aggcaaacgg cacgccgagc aacaagttgc cgaaggcgat accgaagact accggcggca
    10801 ccagactgcc gatgaccaga ccggcgtccc acattttacg ccagcgtgca tcggcgattt
    10861 ttccgcgata atcaaaggcc agcgggcgga agaacagtga gcacagcacc aggatcatcg
    10921 ccacataaaa gccggaaaac gccgctgcat acactctggg ccaggcggca aataatgccc
    10981 caccagcgag gatcaaccag acctggttgc cttcccagtg tgcaccaacg ctgtttatca
    11041 ctatccggcg ttcatcatca ttacgcgcca ccagcggcag cagacagccg atccccatgt
    11101 caaatccgtc ggagatcata aagaccacca ggatcacgcc aatcagcagc caccagatga
    11161 agcgcaatgt ttcataatca aacatgattt tcccctttac ccctgttgct gcgtcggttg
    11221 ttcactctgc atcgcgctcg gccccagacg ggcatatttc tgcatcaggt agacttcggc
    11281 gattaagaac agggtgtaaa gccctacgat catgatcagt gagaaagcca gttgtcctgt
    11341 ggttaaagcg gagtgcgcgg agtatgtcgg taagatgtcc tgtatcgccc acggctgacg
    11401 accaaactcg gtcataaacc acccggcttc aatcgcaatc cacggcaacg gcaaactcca
    11461 gagcgccatt ttcagcaccc agcgatgctg gtcgattttg ccacgcagcg tctggacaag
    11521 cgcaatcagc atcaccagta gcggcaggga accacagccc accatgatgc ggaaactcca
    11581 gaataccggc gcaacctgag gtatcgcgcc acgcatcgcc gcctggtact gtgcggctgt
    11641 gacatgattc atatccggcg catagcggga gagcaacatg ccgtagccca ggtcaccttc
    11701 cagttcccgg aatgcctgca acacatgcgg ctcacgattg ccttgcgata tttcctgcat
    11761 taacagccag gccatacgtc cgcgttgcaa gcgtgggtag gtttcagcca tcaaattttt
    11821 cagacccggc acggttttat ctaatgagtg agtggcgagg atccctaaca gcgcgggaat
    11881 tttgatggca aaggcgttac gctcttgatc ctgttccggc caggcaacca catggaacgg
    11941 tgcaggagca ggttccgttt gccactcccc ttccatcgcc gccagtttta ccggttgtac
    12001 ttgcgcgact tcatacgcag aactgtctcc gagttgtagc gtgccgataa tcgccagagt
    12061 accgaagacg gaaccgatgg caaacgagcg taatgcaaca tcgcgctccc gtccgcgcag
    12121 taaataccag gcgctgatcg ccataataaa catggccccg gtcacgtagc ccgccattac
    12181 ggtgtgcaca aatttcacct ggctgaccgg gttaaagacc agctcgctga agctggtcat
    12241 ctccatacgt agggtgtcga tatcaaaatg cgcaccggtc gggtattgca tccaaccgtt
    12301 ggcattcaat atccacaatg cagagagatt tgaaccgaac gccaccagcc acgtcaccag
    12361 caggtgctgg tatttattca gccgctgcca gccgaagaag aaaagcccga caaaggtgga
    12421 ttcgaggaag aaggccatta atgcttccat cgccagcggt gcgccaaaaa tatcgcccac
    12481 atagttggaa tagaatgacc agttagtacc aaactgaaac tccatggtca ggccggtagc
    12541 cacgccaaga gcaaagttga taccgaagag cttaccccag aagcgcgtca tatcgcggta
    12601 gattgttttg ccggtgacca cgtaaatggt ttccatgata gccagcaaaa aaatcagccc
    12661 cagggtaagg ggtacaaata aaaagtgata cagcgcggtc agagcaaact gccagcgcga
    12721 taaatcaatg acatcccaca tgcgcactcc tgtaggcgaa gagagaaaaa taacccgata
    12781 atgttagatc tccctcttta tcctgaaact ctacgaccag aggtattcgc cgattgcgat
    12841 attcataaga ctgaaagcga tacttaccgt ctttttacgt cggtgcagct tccgccagcc
    12901 actgacatac ctcgctaagc cgctgcgcag agtcgaccaa atcttctggc gctgccagca
    12961 ccacttccgg tattgggcag atttcataac tttccagtaa aggacctttt aaggtgtccg
    13021 tacagcgtaa atgccagaca tggcgtaacc ccgtggcgtt gatatagctc tcgccatagc
    13081 caatggtacg aatctgaata tttcccggcc cacagagacg tgagagaaaa agccgatcag
    13141 cctcgctgat gggtaattgc gtcagattaa tgctgtgcgg ctgcgcgtcg gggttacgta
    13201 catgcgccag taactcatgc gccaacggta ggccattcat caggccatca atgggcggcg
    13261 gtaacagcga atctgtcggc aagagatttt gcgtggccgc ctgccacaac gccagcggcg
    13321 cgcagccggc ctccagtttg tcctccagca acttttcgcc gcgacgtttg cgcacccgcc
    13381 ataatccgca gaagatcgcc tcctgtattt cactttcgct gtcgtcagcc tgctgaatgc
    13441 gcactgacac ttccccttcc ccgagaagcg tattgataaa gtgacgatcg tcggcgttga
    13501 gatttaacag atcgactgcc agcacctcgc ccaacggcgg ctggcgttga cttagttgat
    13561 gttgtaactc gtttaacagc gcaatcacct gcgggctgtg agcacattgc tccagggccg
    13621 ccatactcgg ttcatcattc acctgacagc tgatcggcag tggattcatg ctgaaactgt
    13681 catcgttcgg ttgcgttcct ggccccagca gatggaaaaa agtttcgctc atgaggcacg
    13741 ctcctgttgt ggttcgacaa gcccacgcat caggtttatc agttccgctc acgggtgaat
    13801 accattcagc acaccgcgat agtttccgcc ggtaaacact aatgtggcgg gaaagcgaaa
    13861 gacgccaaag cgatctccga tggcttcgct ctgttcaagg tcagcaatcg ccacctgcca
    13921 tgtgtagccg ggaaactcgc gcagtaattc gccaatcatt accggattat cgctgacctc
    13981 tggcgtgcgt ttcgggtcac tgcttaataa caccacgccg tctggcgctt gcgtaagcca
    14041 gtcgtcaaga cggaattcac taaccggcgt ccagccgcgc gccagcattc gttgccacaa
    14101 cgcatcaaat ggcgtgtcgt tgctcatccc tgctcctcca gtgtcatcct gtactggcgc
    14161 aagcgaacgc cttcgtaatt ttccatcgac agacagtcat aattgagaca gcgcgattca
    14221 ttagccggtt gcggcacaat accccatgca gccagctgcg ccagcgccgc ctgttccgct
    14281 gcgggcagtt gctcccgtgc cagttcgctc aggcggccgc cgtagtcgtc gagcattgcg
    14341 ggttgcagac cgacgagggc aatatgtgct ggcagatgtc cgcggatatc tgccagcgcc
    14401 agcacttcgg agaaactgtt ctggtgcagg ctcattttct tcgcgctgag ataagccgga
    14461 atacgttctc cggcataggt tcgcagcgtt ccaggttcca gcccgtagtc aatggcatcg
    14521 agaatcaaca gatggctggc gctttcgaca taccccagca agttcagtcc ctgagtaccg
    14581 ccatcgacaa tctccacata ctcgggccag tggtaatggg catacaaccg ttccgccacc
    14641 cgcacgccga aaccttcatc ggcccacagc aggttgccca gccccattac cacaacgcgt
    14701 tgctcgctca tgaacgctcc ttgttactta ttttgccaaa tttgtggcta cggtagccgt
    14761 tgaccatggt ggagatcacc gtgtcgtcgg acatgatgtc ttcacgcagc gccatataga
    14821 catgaccgat cacaaacgcg ccaatcagcc acatccccag ccgatgccag ctgtgaatgt
    14881 ccattgagtt gcctcccgtc cagtagaaaa attccaccac ataacggaac ggcgcaaaaa
    14941 tagcgtactg gctgtgttcg ctgtacagcg caaagccggt gatgatcata aagaccgaca
    15001 tcaggaaata gccgaacatc gccgcctggg cgatggggtt atggcctata tcggcactcg
    15061 gacgttttgc cagaacagat accagcggat ttcataccac acgccctgcc accagctttt
    15121 acgccatacc ggcacgataa acagctcgcg ggagtatcga ttgccaacaa aagcccagta
    15181 gatccgcatc agcaagacca cggtaaaaac catcccggcg ctgaagtgaa ttaacctgat
    15241 gtagcccata tagaacagat acgtcgcctc gccgctgacg gaaggtagcg gcttgccgat
    15301 aaagtatccg gtgaccatca acaccgccat gcataacacc gtcagccagt gccagatgcg
    15361 gactggcgct tcaaagacat agtggctgac aacgttgtcg cttttctgtt gcatgatgat
    15421 tctccttcgc tgttaacgca cctgcacgga gatcagctcg ctaccgtcgt cgcccagcac
    15481 gtgtgttgaa caggcgaggc acgggtcaaa gctgtgcaga gtacgcagga tctccagcgg
    15541 ttgctcgggg atcgccattt tggtgttcat cagcgccgct tcataagcgc caatctgccc
    15601 tttgggatcg cgcgggctgg cgttccaggt ggtcggcacc acgcactggt agagatcaat
    15661 cttgccatcg cgaatggcgg cccagtggcc taacgccccg cgcggcgctt cggtaaagcc
    15721 gacgccacgg cactctgtcg gccaggttgc aggttcccat ttttccgtgg aggcggtggc
    15781 aagattgccg tttttcaggt tagtcatcag cttgttgaag aaatattgca acttacctgc
    15841 ggcccactgt gcttcgtgcg cgcggcacaa aatgcggccc aacgttgact ggataccgga
    15901 aagcggcagg ttcagcgccg acatcatgcg atcgaccgac tcaacggtcg cagcatcgcc
    15961 tttgtgataa gcgattaacg tgcgtgccag cggccccact tccatcgcgt taccgcgcca
    16021 gcgcggcgct ttgatccacg agtagcgttc ctgttcattc agctgctgaa tgttggtatc
    16081 gctgcctttg acatcgccgg ggttgtacca cgggtcggta atgccatcga atggatgacg
    16141 cccgacctga tcgttgggat aacgatacca ggcgtggtcg acaaactcct gcacctgctg
    16201 cggatcaacc aaatccactg gcagcacatt gttgaagtcg ccgttaatca ccgcgccgcc
    16261 aggcatcagc agacttttct caccaaagtc gttggcaata tccgggaacg caccgtagct
    16321 gaggacacat ttatcagaaa gacccgtgcc gatttcgctc cacggtttgt tgaactgacc
    16381 gatggctaag gcgtcgggga tcatcacgtt gttaatgaag tccgccgtgc gggtgataat
    16441 cgactgcacc aggttcaggc gttccatatt gaccgccccg accgcgccgc tttcgtcaat
    16501 gttaatggcg caaggcatcc cgccgacaat ccagtttgga tgcgggtttt taccgccaaa
    16561 gaccgcgtgg attttgacaa tttcacgctg gaaatcgaga gcttcgagat agtgggcaaa
    16621 gcccatcagg ttagcttctg gcggcagttt gtactgcggg tgcccccagt agccattgcg
    16681 gaagatcccc aactgcccgc cttcaacaaa ttttttcagg cggttttgta cgtcgaagaa
    16741 atagccaggg gatgatttcg gccaagagga gagactttgc gccagttcgg aggttttccg
    16801 cgggtcggct ttcagcgcat ctaacacatc gatccagtcc atcccggcaa gctgatagaa
    16861 gtgcaccaga tgatcgtggc accagagcgt tgccagcata atgttgcgga tgatattggc
    16921 gttgtccggc actttaatac cgatagcatc ttcgatggcg taaaccgaag ccagggcgtg
    16981 tacgccagta cagacgccgc agatacgttc aacgaacgcc cacgcatcgc gcgggtcgcg
    17041 cccttgtagg atgatctcca gcccgcgaaa catggtgccg caggagacgg cattggtgat
    17101 cacattctga tcgttaatat tcacttcgca gcgcatgtgg ccttcgatgc gcgtaatcgg
    17161 gtcgaccacc aggcggcgtc cggcattatt gatggtgtat ccctgagttt cgtactgagt
    17221 gctcatgcct gtttatcctc attgcctggc tgatgttcgg tttctgtagg ttgctggtta
    17281 tgacgtctgc gctggtcaac ggcgctggcg actgcgtgca caccaacagc cgctgccacc
    17341 acaccaagcg cggttaaacc gacggtatcg gcggtggaat gagtacccat ttgcggaata
    17401 tcgaccacgc ggctgtagaa cgaaccgcaa tcccagaaac cattttccgc acagcccagg
    17461 cagccgtgac cagactggat tgggaaagaa acgccatcat tccagcgtgt ggaggaacag
    17521 gcgttatagg tggtaggccc tttgcagccc attttgtaca ggcagtaacc tttgcgggca
    17581 gcgtcatcat cccaactctg gacgaactct ccggcgtcga agtgggcgcg gcgatagcat
    17641 ttatcgtgga ttcgctgacc atagaacatc agcggacggc ccattctgtc gacatctggc
    17701 aagcgatcaa aggtcaccat gtaagtaatg atggcgctca tcacatccgg gatcggcggg
    17761 cagccaggta ctttgataat gggtttgtcg gtgatgactt tgtcgatagg cgttgcctgc
    17821 gtcggattgg gccacgcggc ctgcacgcag ccccaggacg cgcaggttcc ccaggcgata
    17881 atcgcgctgg ctccggcagc ggcacgtttg agtttctcaa taaacggtcg accgctgctg
    17941 atacagaaca tcccctgctc gcccagcggc ggattacctt ctactgcgag gatatatttg
    18001 ccattgtatt gcgtgatgat gtcttcgaag acttcttccg cctgggttcc ggcggcagcc
    18061 atcaaagtat cgtcataatc gagggaaatc agggaaagga tgacgtcctt cgccagtggg
    18121 tgagcggagc ggataaaaga ttcggtacag caggtgcatt ccagaccgtg gatccatacc
    18181 accggaatgc ggggtttgtt ctccagcgcc caggcaatct ttggtgccat tcccgcgcct
    18241 aatcccagcg acgtggcagc cagactacaa tatttgagaa agctgcgccg ggtaacgccc
    18301 tgacgccgca tggcctggta aaatgtttcc tcgttattca tatcgcacgt ctcttctcct
    18361 tgcgacaccg gcaggacacc gggcgcttat ggtcggcaag cgacgtccct gtagcaaggc
    18421 aaaccatcgc ttcacgtgag atgctgaaaa cgaaagctca tccttctgca ctggcgcacg
    18481 tcgccagaaa gtattgttaa taaagcgtag tgaaa
//
LOCUS       DAZPDU010000081        18213 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZPDU010000081 DAZPDU010000000
VERSION     DAZPDU010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18213
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.082"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            15..1091
                     /locus_tag="ACHURS_002476"
     CDS             15..1091
                     /locus_tag="ACHURS_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1238179.1"
                     /translation="MQRKKYDPNLPRNLTYRRRDKAYYWRNPLTKEEFTLGKISRRDA
                     VTQAIEANHYIYKNYSPAALIEKLKGFDSFTMADWIERYKTILIRRKVSRNTYKIRAN
                     QLETIKEKLGEILLTEITTRHIAEFLDLWIEGGKNTMAGSMRSVLSDMFREAIVEGRI
                     SQNPVTPTRAPKIVVTRERLKLKIYNCIREAADQLPAWFPLAMDLALVTGQRREDITN
                     MRFSDIYDDRLHVRQIKTGMMIAIPLSLSLPVAGLRLGTVVERCRLVSRGDYLISAGI
                     RKNSPDGSIHPDGLTKKFVAARKFTGIQFSENPPTFHEIRSLAGRLYKETCGEEFAQR
                     LLGHTSEKTTKMYLDEREKTYLLL"
     gene            complement(1484..1825)
                     /locus_tag="ACHURS_002477"
     CDS             complement(1484..1825)
                     /locus_tag="ACHURS_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFR1238180.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(1838..2710)
                     /gene="copD"
                     /locus_tag="ACHURS_002478"
     CDS             complement(1838..2710)
                     /gene="copD"
                     /locus_tag="ACHURS_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFR1238181.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGMGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMCFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQEEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(2714..3088)
                     /gene="yobA"
                     /locus_tag="ACHURS_002479"
     CDS             complement(2714..3088)
                     /gene="yobA"
                     /locus_tag="ACHURS_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFR1238182.1"
                     /translation="MASTARSLRYALAILTTSLVTPFVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3227..3457
                     /gene="holE"
                     /locus_tag="ACHURS_002480"
     CDS             3227..3457
                     /gene="holE"
                     /locus_tag="ACHURS_002480"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFR1238183.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3559..4215
                     /gene="yobB"
                     /locus_tag="ACHURS_002481"
     CDS             3559..4215
                     /gene="yobB"
                     /locus_tag="ACHURS_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFR1238184.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPEGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            4239..4901
                     /gene="exoX"
                     /locus_tag="ACHURS_002482"
     CDS             4239..4901
                     /gene="exoX"
                     /locus_tag="ACHURS_002482"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFR1238185.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(4898..6958)
                     /gene="ptrB"
                     /locus_tag="ACHURS_002483"
     CDS             complement(4898..6958)
                     /gene="ptrB"
                     /locus_tag="ACHURS_002483"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFR1238186.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPCENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTDERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(7167..7826)
                     /locus_tag="ACHURS_002484"
     CDS             complement(7167..7826)
                     /locus_tag="ACHURS_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFR1238187.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(8153..8509)
                     /gene="yebF"
                     /locus_tag="ACHURS_002485"
     CDS             complement(8153..8509)
                     /gene="yebF"
                     /locus_tag="ACHURS_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFR1238188.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(8576..8866)
                     /gene="yebG"
                     /locus_tag="ACHURS_002486"
     CDS             complement(8576..8866)
                     /gene="yebG"
                     /locus_tag="ACHURS_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFR1238189.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            9000..10178
                     /gene="purT"
                     /locus_tag="ACHURS_002487"
     CDS             9000..10178
                     /gene="purT"
                     /locus_tag="ACHURS_002487"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFR1238190.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVADIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWKYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(10234..10875)
                     /gene="kdgA"
                     /locus_tag="ACHURS_002488"
                     /gene_synonym="eda"
     CDS             complement(10234..10875)
                     /gene="kdgA"
                     /locus_tag="ACHURS_002488"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFR1238191.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(10912..12723)
                     /gene="edd"
                     /locus_tag="ACHURS_002489"
     CDS             complement(10912..12723)
                     /gene="edd"
                     /locus_tag="ACHURS_002489"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFR1238192.1"
                     /translation="MNPQLLCVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSLGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEQ
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(12958..14433)
                     /gene="zwf"
                     /locus_tag="ACHURS_002490"
     CDS             complement(12958..14433)
                     /gene="zwf"
                     /locus_tag="ACHURS_002490"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFR1238193.1"
                     /translation="MAVTQTTQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            14771..15640
                     /gene="hexR"
                     /locus_tag="ACHURS_002491"
                     /gene_synonym="yebK"
     CDS             14771..15640
                     /gene="hexR"
                     /locus_tag="ACHURS_002491"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFR1238194.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            15768..17210
                     /gene="pyk"
                     /locus_tag="ACHURS_002492"
     CDS             15768..17210
                     /gene="pyk"
                     /locus_tag="ACHURS_002492"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFR1238195.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(17270..18112)
                     /locus_tag="ACHURS_002493"
     CDS             complement(17270..18112)
                     /locus_tag="ACHURS_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFR1238196.1"
                     /translation="MHHIKTAADLNCFLNSMVAVSQPTVDKIIFGAEATLINKIGTCW
                     IASMDVLRKAVFEGVNIIITHEPTFYSYADLEGDDLEFSWARKIMDYTRGELSYLKII
                     EQKKEFLHKNNLVIIRCHDVMDREPTFGMSKALAQQLELDVTNIVASDEMYCVYAIDP
                     DSAINITKRFAKNLKIYNLPGIQFYGDKARVVRTVGIGAGCFCDPIQYMEYQADYYIT
                     INDSIKTWVQTQYSKDSGLPMAVIGHSVAEEAGMRRLASYLDLHSGYPCIHFTGGCDY
                     DWIE"
BASE COUNT         4670 a         4574 c         4393 g         4576 t
ORIGIN      
        1 ggagaagtta aaacatgcag cggaaaaaat acgatcccaa tttgcctaga aacttaacat
       61 atcgaaggag agacaaagca tattactggc gcaaccctct gacgaaagaa gaatttacac
      121 taggtaaaat ttcaagaaga gatgcagtaa cgcaggcaat tgaagcaaat cattatatat
      181 acaaaaacta ctctcccgct gccttaattg aaaaacttaa agggttcgac tcatttacta
      241 tggcagactg gattgaacgt tacaaaacga ttcttataag gagaaaagtg tccagaaata
      301 cttataaaat tcgggcaaat caactggaga caataaaaga aaaattggga gagattttac
      361 tgacagaaat aaccactcgc catattgccg agtttcttga tttgtggatt gaaggaggga
      421 aaaacacaat ggcaggatca atgcgttctg tgttgtctga tatgtttcgc gaggccattg
      481 ttgaaggacg tatatctcaa aatccagtca cgccaacaag agcaccgaaa atagtagtta
      541 caagagaacg actgaaacta aagatataca actgcatcag ggaggcagca gatcaacttc
      601 cggcatggtt cccattagct atggatttag cccttgtaac aggacaacgt cgcgaagaca
      661 taacgaatat gcggttcagt gatatttatg atgatcgtct ccacgtcagg caaattaaga
      721 caggaatgat gattgctatc cccctgtcac tcagccttcc tgtcgctggt ctacggcttg
      781 gtacagtagt tgaacgatgc cgcctggtaa gccggggaga ttatctaatc agtgccggga
      841 ttagaaaaaa cagccctgac ggcagcattc acccggatgg cctgacaaaa aaatttgtcg
      901 cagccagaaa attcacaggt atccagttca gtgaaaaccc accaactttt cacgagatca
      961 gaagcctggc tggacgattg tacaaagaaa catgtggaga agaatttgct cagcgtctac
     1021 ttggccacac atcggagaag acaacaaaaa tgtatcttga tgagagagaa aaaacgtact
     1081 tactgctctg attttaacgt aaatggattg ttaaatgtat tttggttgtg atataaccaa
     1141 aaagaccgga atacagaaat tcgagtaaat ttcggggaat ttcgggggag acatttgcaa
     1201 ctaattgatt tcaaatgcaa ttaaaaaaag accgaatacg attcctgtat tcggtccagg
     1261 gaaatggctc ttgggagaga gccgtgcgct aaaagttggc attaatgcag gcttagttgc
     1321 cttgcccttt aagaatagat gacgacgcca ggttttccag tttgcgtgca aaatggtcaa
     1381 taaaaagcgc ggtggtcatc agcttaaatg ttaaaaaccg cccgttctgg tgaaagaact
     1441 gaggcggttt ttttattgga aatcaaaagg ctattttagg taattaacag agtttttcag
     1501 ctcgttctat aaacggtgcc agactcattt tttcgccggg attgttagga tcatcaatct
     1561 gaatcaccga aatgggttgg gcattggtct tcccactggc aacttccttt tgtgcgatat
     1621 cgtttaaagg atactgcacg agggtactcg gattaataac atacaaagca ttacccggtc
     1681 ggcaagtcag catcacctct tcgcgattaa acgcccattt gtctttaccc acttcaaaac
     1741 gactgacggt aatcacctgc ggtgcagcca gcgccgctgc agaactggtg agtaacagaa
     1801 acgccagaat actttttttc atcattttcg taatccatca aaaaggttcg agagtcgcaa
     1861 gcaggctgat gatcaccagc actaccgcac ctatcgccca ttcgagttta gtcatccaca
     1921 caaaccaggg tgccgcacga tcttcctcct gcctcatgcg cggtacaaga acataacgat
     1981 tagccagcgc aatgaccacc ataatcatca ccaggatggc tttgagcaat aagagttggc
     2041 cccagtaagt cgtaagcgtg gggggaaatc cggtaatcaa caatgcatta agcacgccgc
     2101 ttgccagtac gccgatcacc gcaaaatgcc cgcaccatga aaaacacatc agcgcctgaa
     2161 tagcctggtg tcgccaacgg cctttgatga gttgcataca ccagagcacc ggcagtaatc
     2221 ccccaaacca cgccgccgca caaatcaggt gaatcgcgtg attagtctga tggattttcg
     2281 ccgttacccc ttcattcagt gtcgcatgcc ccattcccgc cagcagaata aattgcgcgg
     2341 tagtgagcat aaacagcaaa cgtggcatat tccgcggttg cataagggcg acgatcaacg
     2401 ttacgagggc gagaacaatt tgccataacc agacgccacc aaactgcgtt tgcaaaacag
     2461 cctgccaaat gtttggcgaa aatacatctg tccatcccgt tcccatcaac ccgccctgaa
     2521 cggcgagcat tgcagtggca ctgatcaaac tccagacggc agcgtgctgt tgtaaacgca
     2581 gaaaacgctt cgccagcaag cgacgaatcg tcaagggggc cagccaggca ccgtacattg
     2641 cgaagccaaa aaccagcatc agcgaggtga aatggataaa tcgcagcgcg atccaggtaa
     2701 acgccagcat gatttatttc acgctaaagg tgtagtgtcc tttcgtttta tgcccgtcca
     2761 ccgaaacaac atgccagtca acggtgtagg ttccgggttt cagcgaatcg gccagtggaa
     2821 caatcagttg cttttggtcc tgctcatttc gcttcgccgg taatgtttta atattttcgt
     2881 tttttggccc cgtgattttt gcaccactga atcctgtttc cacaccttcc gagaagttta
     2941 aagtgattgc ctgcggtgca gctgtcactt gcgcgtttgc cgcaggatac tgatgcgtta
     3001 aatgcgcatg tgcccagacg aaaggtgtca ccaacgaagt ggtcaggatt gccagcgcgt
     3061 agcgaaggga gcgtgcagtt gaagccatat tatctattcc tttttgtaat aactttttta
     3121 cagagcataa ccttgtctaa tgtctgagtc gaggatcatc aattccggct tgccatcctg
     3181 gctcactctt agtaactttt gcccgcgaat gatgaggaga ttaagaatgc tgaagaatct
     3241 ggctaaactg gatcaaacag aaatggataa agtgaatgtc gatttggcgg cggccggggt
     3301 ggcatttaaa gaacgctaca atatgccggt gatcgctgaa gcggttgaac gtgaacagcc
     3361 tgaacatttg cgcagctggt ttcgcgagcg gcttattgcc caccgtttgg cttcggtcaa
     3421 tctgtcacgt ttaccttacg agcccaaact taaataaaac ttatacaggg ttacactttc
     3481 ttacataacg cctgctaaat tatgagtatt ttctaaaccg cacccataat ttgcagtcat
     3541 tttgaaaagg aagtcattat gtcgttctgg aaagttgcag cagcgcaata tgagcctcgt
     3601 aaaacctcac tcaccgaaca agtagctcat catctggagt ttgtgagggc cgccgccagg
     3661 caacaatgtc agctgttagt ttttccatcg ctttctttac tggggtgtga ttattcgcga
     3721 cgtgcccttc ctgccccacc cgatctctca ctgttagacc cgctttgtta tgccgcaacg
     3781 acctggcgaa tgaccatcat tgccggcctt cccgttgaat ataacgatcg ctttattcgt
     3841 ggaattgcgg tgttcgcacc gtggcgaaaa acgcctggga tctaccatca gagtcatggt
     3901 gcatgtctgg gccgtcgttc cagaaccatc acggtggtcg atgaacaacc ggaaggtatg
     3961 gatatggacc cgacctgttc actgtttacg acagggcaat gtctgggaga acccgacctg
     4021 ctggcgtcgg cccgtcgctt acaatttttt tcacatcagt actcgattgc tgtgctgatg
     4081 gcaaatgccc gtggtaacag tgcactatgg gatgaatatg gtcgtctcat cgttcgcgcc
     4141 gatcgtggtt cattattgtt agtcggtcag cgttcatcac agggttggca aggcgatatc
     4201 attccattac gctaggcttt ttcggcctgg agtatgccat gttgcgcatt atcgatacag
     4261 aaacctgcgg tttgcaggga gggatcgttg agattgcctc tgttgatgtc attgacggaa
     4321 aaatcgtcaa ccccatgagc cacctggtgc gccccgatcg tcctattagt ccacaagcga
     4381 tggcgattca tcgcatcacc gaagccatgg tcgccgataa accgtggatt gaagatgtga
     4441 tcccacacta ttacggcagt gaatggtatg tcgcgcacaa cgccagcttt gaccgccgcg
     4501 tactgcctga gatgcccggt gagtggattt gcaccatgaa actggcccgt cgcttgtggc
     4561 caggaatcaa gtacagcaat atggcgttat ataaaacacg gaagctcaat gtacagacgc
     4621 cgccgggcct gcatcatcac cgcgcgttgt atgactgtta tatcaccgcc gcgttgctta
     4681 tcgatattat gaacacctcc ggctggacgg cagaacagat ggccgatatc accggacgtc
     4741 cgtcgttgat gacgaccttc acctttggca aataccgtgg caaagcggtt tccgacgttg
     4801 ccgaacgcga tccgggctat ctgcgctggt tatttaataa cctggacagc atgagcccgg
     4861 agctgcgttt aacactgaaa cattatctgg aaaatactta atccgcaggc gtagcgggta
     4921 atgttccctg cgccagcgcg accagaaaag catattccat cgctacgcct tcgtacgatt
     4981 taaagcggcc agatttaccg ccatggcctg agtccatgtc ggtacagagc aataaaagat
     5041 ggttatcggt tttcagctcg cgcaatttag cgacccattt tgccggttcc caatattgca
     5101 cctgagaatc gtgtaaaccg gtcgttacca gtaaatgcgg ataagcctgt gcggtgacgt
     5161 tgtcatacgg gctgtagctt ttcatgtact cgtaatattg cggatcctgc gggttacccc
     5221 actcttcaaa ctcaccagtg gtaagaggaa ttgattcatc aagcatcgtt gttacaacat
     5281 caacaaacgg tacctgggcg ataacgccgt ggaataattc cgggcgttga ttaattgcaa
     5341 cgcccatcaa catgcccccc gcactcccgc ccatcgcata acaaagcgaa ggagagccat
     5401 agcccagttt taacaatgca tcgcaggcat caagataatc attaaacgta tttttcttct
     5461 tcagaaattt tccgtcttcg taccattgtt gccccagctc accaccgccg cgaacatgga
     5521 caatggcgta gacaaagcca cgatctaaca aactcaagcg gctaaaactg aaatcggcat
     5581 caatacttgc gccgtaagaa ccatagccat acaccagcaa cgggttgtgt cctttgcgaa
     5641 aatgtttgcg atggtagacc aacgaaaccg gaacttcgac gccatcacgg gcgactatcc
     5701 acaggtgttc actgcggtaa ttcgccgcat caaaaccagg aacttccgtt tgttttaata
     5761 cacgacgctc atcggtatcc atatccagtt caaacaaagt gtctggtgtg gtcatggaag
     5821 aataaccata acgcaatcgc gcggtttcag gttctggatt gtaggcaatc caggtcacat
     5881 aggctggatc atcaaaggca ataccaatga cttcccgggt cttgcggtta atttggcgca
     5941 aactggttaa cccgcgctga cgctcttcaa ccaccagcca gtcggtaaac agcgtaaacc
     6001 cttccagcat gatgttttcg catggcggaa ttaactcttc ccactgttgc tcatcacgca
     6061 tacgggtacg gtataagcca aagtttttgc cgtggcggtt ggaacgcaga taaaaacgat
     6121 gctggtagtg atcaaggctg tattcgtgat ctttgcggcg cggcagaaaa acaaacggct
     6181 cggcatcggc catttccgcg tccagcaggc gaacttcact ggtggtggcg ctggccaaat
     6241 gaatgactac atagtgcttc gaggtcgttt tatgcaggct gacgtaatag gtatcgtctt
     6301 tttcttcgta gatcagttta tcttgcgatg ctggcgtacc gatggcgtga cgccagacct
     6361 gataaggcag cagcgtcacc ggatgcttgc gaacatagta gaaagtccag gagtcatttg
     6421 cccagacaaa gctgggttca acgttatcca gcagttccgg gtaccagtta ccagtttcca
     6481 gattacgaaa acgaatgccg tactggcgtc gggaaagaaa atcttctgcc agcgccataa
     6541 tggtgttatc gggcgtaatc gccattccgc ccatcgaata aaactcacta tgagctgcgc
     6601 gcttattggc atcgagcaat atttcccact catcccactc ttcactgaat gccgattgac
     6661 gctggtagat agcatattca cagcctggtt cataaatatg ccgatagcgg tagccatttt
     6721 tgatgtaggg cgcagaaact tctcgttgcg gaatgcggtc gatgatttcc tttaagatgc
     6781 gatcctgcaa ggcttgttgt gaggccatca cccgatgacc gtaactattt tcttgttgca
     6841 ggtagtccag gacttctggc tgagaacgcg tatcatcccg cagccagtag taattatcga
     6901 tgcgcgtatc gccatgaagc gtcatggcgt ggggaatgcg ggcggctttt ggtagcatgt
     6961 tattgttctt tctggttgaa acatcttata agggtggcaa aactcaccgg ggatgcaagc
     7021 gaaacagggg agtcattgct tagatgatga caggtaatgg cacggatatc gaatgttatg
     7081 cagacagaga aactcagcct gttcaaatgg ctgtgcggtt ctggatagcc cgaaatagtc
     7141 aacttcaggc tatccagaga gcggaattat tccgccaaag tgcgtttttg ctgttcgaga
     7201 tcgcgttcaa tgccgtcacg aacatcctgg gggattttca gcgcgtcacc cagtgcattc
     7261 aggtaactgc gttccataaa atggtcaata tcaatagccg cgcaactcag gaaatagatt
     7321 tccagcgcct cttcttcatt gcggaccccg gtagccaggc gttgtggatc cagcggttgt
     7381 tcgattgcct gctcaatgag tacacgcccc tgctcttcca cgccggcttc acgcaattgc
     7441 tggtcgattg ccgcacgttc tttggcatca atatgaccat cacttttagc ggcaaagacc
     7501 agcgcaagga tcaaacgttc tgtacgctca tccagcggcg tactttgcgc gccaaactgc
     7561 ggttcgtcct gatgcgccgc gcgaatttta tctttgtatt tattccacag caccgtaccc
     7621 gctaccgctc cgccgccaac cagtaacgcg ttggtgccat atttcgtaag aagtttacgt
     7681 gctgatttat ttgcgaccag cagcccagcc agtccgccta atgcgcctgg cactaacagt
     7741 ttgcccaatc cctgatccgc agacgaggag gtagaagaac tgctttgccc aagcagggat
     7801 tgcagttgat ttaaccagtt agccatattt gctcctcaat aaccattttt agtgcctgta
     7861 gcatagcgga tgaggatgtc agaaaatgta tatcgagaca aaagatgcgc aaataagcgg
     7921 ggcggtaggc cgttattcaa agaaattcgc gccaggtaag gtatttactg tatatcaggt
     7981 gataagggta ttttaggtga aagtagtttg tcgttttgag tggcattgtt tgatgctgat
     8041 tgcccgatgc gacgctgttg cgtcttctca ggcctacgat tgtggtgcgt tctggggcgg
     8101 acaaggcact cgcgccgcat ccaccaacgg tcaatcgaat tatcagaagc cattaacgcc
     8161 gctgatattc cgccattccc gctttgcagt ccacgctgac ctggtaatga atatcggcac
     8221 ttttaccacg cacggttagc ggtactgacc atttatcatc tttaccctga atgtcctgca
     8281 aactgaccca agccacggga tcggcctgac cgacaatttt ttgatcatct gcccaacgcg
     8341 ccacgcgatt ttgttgataa tcacgtttta cgctcgcggc aattccggca gcatccagac
     8401 cttcacactt tgggaaagtg accgacttgc tggtttcatt attggcagcg aaaactgatg
     8461 cgcaggcaga aaccaacaac agccctaaaa acgcccctct ttttttcatg tttttctcca
     8521 tagcacaatg attcaggaga aagcatggta caaattgtca ggagcgcaag ttgcttcagg
     8581 ccgcgtgtga ggcatcttcc tcttcggatt cagcaccgac gacctgtggg gacggtaatt
     8641 tgccggtttt cagaatggtg ctcagcacat ctttttgttc tgccagccat agcgaaaggg
     8701 cttcacgttg ctcgtcttcc atttgcactg gagaatttgt cagccaggtg tcaagcagat
     8761 ccgccgtatc aagcattttg tcataagcat cggcttcctt tttgctggta aacgacattt
     8821 tctcttcgcc ctcacgaatg actacgtatt taacttcaac cgccatttgc agcctctcat
     8881 aataactgtg attttataca gtatatttct tttcggttga gaaatcaaca tcagcaataa
     8941 agacacacgc aaacgttttc gtttatactg cgcgcggaat taatcagggg atattcgtta
     9001 tgacgttatt aggcactgcg ctgcgtccgg cagcaactcg cgtgatgtta ttaggctccg
     9061 gtgaactggg taaagaagtg gcaatcgagt gtcagcgtct cggcgtagag gtgattgccg
     9121 tcgatcgcta tgccgacgca ccagccatgc atgtcgcgca tcgctcccat gtcattaata
     9181 tgcttgatgg tgatgcatta cgccgtgtgg ttgaactgga aaaaccacat tatatcgtgc
     9241 cggagatcga agctattgcc accgatatgc tgatccaact tgaagaggaa ggactgaatg
     9301 ttgtcccctg cgctcgcgca acgaaattaa cgatgaatcg cgagggtatc cgtcgcctgg
     9361 cggcagaaga gctgcagctg cccacttcca cttatcgttt tgccgatagc gaaagccttt
     9421 tccgcgaggc ggttgctgac attggctatc cctgcattgt aaaaccggtg atgagctctt
     9481 ccggcaaggg gcagacgttt attcgttctg cagagcaact tgctcaggca tggaagtacg
     9541 ctcagcaagg cggtcgcgcc ggagcgggcc gcgtaattgt tgaaggcgtc gttaagtttg
     9601 acttcgaaat taccttgcta accgtcagcg cggtggatgg cgtccatttc tgtgcaccgg
     9661 taggtcatcg ccaggaagat ggcgactacc gtgaatcctg gcaaccacag caaatgagcc
     9721 cgcttgccct tgaacgtgcg caggagattg cccgtaaagt ggtgctggca ctgggcggtt
     9781 atggtctgtt tggtgtcgag ctgtttgtct gtggtgatga ggtgattttc agtgaggtct
     9841 cccctcgtcc acatgatacc gggatggtga cgttaatttc tcaagatctt tcagagtttg
     9901 ccctgcatgt acgtgccttc ctcggacttc cggttggcgg gatccgtcag tatggtcctg
     9961 cagcttctgc cgttattctg ccacaactga ccagtcagaa tgtcacgttt gataatgtgc
    10021 agaatgccgt aggcgcagat ttgcagattc gtttatttgg taagccggaa attgatggca
    10081 gccgtcgtct gggtgttgcg ctggcgaccg ctgagagtgt tgttgacgcc attgaacgcg
    10141 cgaagcacgc cgccggacag gtaaaagtac agggttaaac ccggcaaaaa aacgctacaa
    10201 aaatgcccga tcgtagatcg ggcgttttga cttttacagc ttagcgcctt ctacagcttc
    10261 acgcgccagc ttagtaatgc ggtcgtaatc gcccgcttcc agcgcatctg ccggaaccag
    10321 ccaggaacca ccgatgcaca gcacgctttt cagcgccagg tagtcacggt agttagccgg
    10381 agaaatacca cccgtcgggc agaaacggac ctgggagaac ggacccgcga tcgcctgcag
    10441 ggctttcacg ccgccgttag cttcagccgg gaagaatttg aactctttca aaccgtagtc
    10501 catacccagc atcagttcgg aaacagtgct gatccccgga attagaggaa tagtcccttc
    10561 ggtagcagct ttcagcagcg gctcggtcag acccgggcta attgcgaact gtgcacccgc
    10621 ttcagtgact tctgccagct gctgtggatt cagcaccgta ccggcaccca caatcgcttc
    10681 aggcacttct ttggcgatag cacggatagc gtcaactgca cactcggtac gcagagtcac
    10741 ttccagaacg cgcaccccac cagcaaccaa cgcttttgcc atcggcactg cgtgttccag
    10801 tttttttacc acgataaccg gtacaaccgg gccggtggtc aggattgatt ctgcacttgt
    10861 tttccagttt ttcatcagag ttttctctcg cctgattaca aatttgtcgt cttaaaaagt
    10921 gatacaggtt gcgccctgtt cggcaccgga cagtttttca cgcaaggcgc tgaataattc
    10981 acgtcctgtc cccacgcgtg acgcgctcag gtcaggaatg tgcggttcgc gagcagccag
    11041 ttccgcttcg tctaccagca gcgtcagttc gcctgtctgt ccattcacac gaatgatgtc
    11101 cccgtcgcgc acttttgcca gcagcccgcc atcgtaggct tctggtgtta cgtggatagc
    11161 tgacggcact ttacctgaag cgccggagag tcgtccatcg gtaactaacg caattttgaa
    11221 acaccggtcc aataatacac caagtggcgg catgagttta tgtaattctg gcattccgtt
    11281 cgcttttggc ccctgatgac ggacaacaac gacacaatcg cggtccagca aacccgcttc
    11341 aaaggccggc ataacgtcat gctggctttc aaaaacaacc gctggcgctt caatcacctg
    11401 gttctcaacc ggcacggcag aggttttcat aaccgcacgg cccaggttac cgcttaacac
    11461 ttttgtccca ccatgatgag agaaaggttg ttcgaaggaa gcgatcacat tgctgtcgag
    11521 tgatttttcc gccccttccc gccagtcctg ttcaccatta ttcagccatg gttcaagggt
    11581 ataacgagac agaccaaagc ctgccaccgt attgacatct tcatgcagca ggcctgcttt
    11641 gagcagttca cgcaccagaa ccggtacgcc acctgccgcc tggaagtggt taatatcggc
    11701 cggaccgttc gggtagagac gtgccatcag cggtacaaca tcagaaaggt cagagaagtc
    11761 atcccagtta atctgaatac cggccgcgcg cgccatcgcc accaggtgca tggtgtggtt
    11821 agtggaacca ccggtcgcca gcagtgcaac gataccgttc accaccactt tctcatcgat
    11881 catcttaccg atcggcatcc attcattacc attaccggtc atgcgtgtaa cctgacgcgc
    11941 agcggcagcg gtcaaagcat cgcgcagcgg agaatccgga tgaacaaaag aagagcctgg
    12001 caactgcatc cccataaatt ccaccaccat ctggttggtg ttggcagtac cgtagaaagt
    12061 acatgttccc ggcgcatggt aagacgcggc ttctgactcc agtaaggcca tgcggtccac
    12121 tttaccttcg gcataaagct ggcgaatacg cactttttct ttatttggca aaccgcttgc
    12181 catcggtcca gacggcacaa acaccgcagg caaatgacca agcgacaggg ctgccatcgt
    12241 cagacccggg acaatcttgt cgcacacacc gaggaacaga gcaccatcaa acatgttatg
    12301 ggacagcccc accgccgcag acatcgctat cacttcgcgg cttagcagcg acaattccat
    12361 tccatcctgc ccctgggtga caccatcaca catcgccgga acaccgcccg caacctgacc
    12421 aaccgcattc gcttcatgca gggctttacg aatgatttct ggatagtgtt cataaggctg
    12481 gtgcgcggag agcatgtcgt tataggaggt gatgatggcg atattgttac gcaacatgct
    12541 tttcaaagag gctttgtctt ctggctggca ggcagcgaaa ccgtgtgcca ggttaccgca
    12601 tgccaactgc gaacgatgaa cggtcgacgt tttcgcttgt tctatccggg cgagataagc
    12661 agagcgagtc tcgcgcgaac gttcaatgat tcgatttgtt acgcataaca attgtggatt
    12721 cataaaggct cctgaaattg agttgtcaga gcaggatgat tcacaacgcg tttcattcag
    12781 aggatttatg agtgaaacgc ctgtaaccgg agctcatagg gcaaaaacgt ttcagtcagt
    12841 gtaataaaaa aagcctcgtg ggtgaatccg cacgaggcct gaaagtgtaa aaattgttct
    12901 acaatctgtg caagatcatg ttaccggtaa aataaccata aaggataagc gcagatatta
    12961 ctcaaactca ttccaggaac gaccatcacg ggtaatcatc gccaccgagg caacgggtcc
    13021 ccaggttccg gcctgatacg gtttcggcgc atcattgtcc atcgcccacg cctcagtaat
    13081 ggagtctacc catttccagg cttcttccac ttcatcacga cgtacaaaca gtgcctgaat
    13141 accacgcatg gtttccagca gcagacgttc ataggcatcc gccagatgcg tctgattaaa
    13201 ggtttctgaa tagctcagat ccagcttggt gatttgcagg ttatgtttgt ggtcaaggcc
    13261 aggaacttta ttcagtacct ggatatccac gccttcatca ggttgcagac ggatagtcag
    13321 tttattctgc ggcagatcct gccacgactc tttaaacaga ttcagttcag gtgttttgaa
    13381 atagaccacg acttcagaac atttggtcgg cagacgttta ccagtacgca ggtagaatgg
    13441 cacaccggcc cagcgccagt tatcaatgtc gacgcggatc gccacgaagg tttctgtatt
    13501 gctgctcttg ttcgcgccct cttcttccag atatcccggc acttttttgc cctgggcgaa
    13561 gcccgcagta tattgcccgc gtacggtttt ttcgcgtacg ttggagcggt cgatgcggcg
    13621 cagggacttc agtactttca ctttttcatc gcggatgctg tctgcgctca ggtcagatgg
    13681 cggagacatc gcaatcatgc agagaatttg tagcaggtgg ttctgaatca tgtcacgcat
    13741 ctgaccggct ttatcaaaat agccccagcg cccttcgatc cccacttctt ctgccacggt
    13801 aatctcaaca tgatcaatgg tgcgattgtc ccagttattc acaaacaggg agttagcaaa
    13861 acgcagcgcc aacaggttca gcaccgtttc tttaccaaga tagtggtcga tacggtaaac
    13921 ctggcactcc tcgaagtatt cgccaacctg atcattgatt tcctgcgagg tcgccagcga
    13981 cgtccccagc ggtttctcca tgactacgcg tgccggttta gcattcagtt ttgcctcgcc
    14041 aagccctttg caaattgcgc caaaagtgct gggcggcatg gcaaagtagt taatggtgat
    14101 acgatttttt tgatccagca tcgcgccgag acggttgaat gcagcagtgt cattgacatc
    14161 gagattacaa aaatccagac gtgcactcag ggtgtcccat aaaccttcat caatggtttc
    14221 tttcatgaaa gtttcgagcg cctcgcggac aactttggta tatgccgctt tatcccagtc
    14281 agcacgccct acgccgataa tccgggtgtc cgggttgagc tgaccggctt tttccagttg
    14341 atacagggaa ggcagcaatt tacgacgcgc aaggtcgcct ttcgcgccga aaatgaccag
    14401 gtcacaggcc tgggttgttt gcgttaccgc catgtcattc tccttaagtt aactaacccg
    14461 gtacttaagc cagggtatac ttgtaatttt cttacggtgc actgtactgc ttttacgagc
    14521 ttgcgaaaac tgtaaacgct tatccacccg tgcgattacg ggaaaagcgc gcaaagtgcg
    14581 gcaaaactga taaaaaaatc atcgttttcc attcggtaaa aatctgacac tgatcatgtt
    14641 atgaaaaaaa ataacaactt ttttatctgc ttttgtcatt aacacggcac acaggcgtaa
    14701 tatctgacaa aactgcattt cgatttcttt cagtgcggaa atcgtcatta cccgtgagtc
    14761 tctttacatc atgaatatgc tggaaaaaat ccagtctcag ctggaacatt tgagcaaatc
    14821 agagcgcaaa gttgccgagg tcattctggc ttcgcccgat aacgcgatcc attcgagtat
    14881 tgctgctatg gcactggaag ccaatgttag cgaaccgacg gtgaatcgtt tctgtcgcag
    14941 catggacacg cgcggttttc ctgattttaa acttcatctg gcacagagtc tggcgaatgg
    15001 cactccctat gttaatcgca atgtcaatga agatgacagc gttgaatcat acacagggaa
    15061 aatatttgag tccgcaatgg caacgcttga tcatgtccgt cattcactgg ataaatctgc
    15121 catcaaccgc gccgtcgact tgctcactca ggcaaaaaaa atcgcctttt tcggattagg
    15181 ctcttcagcc gccgttgccc acgatgcgat gaataagttc tttcgtttta atgttccggt
    15241 ggtgtactcc gatgatatcg tgctgcaacg catgagttgt atgaattgta gcgacggaga
    15301 cgtggtggtg ctgatttctc acactggaag aacaaaaaat ctggtcgagc tggcgcagct
    15361 ggctcgcgaa aacgacgcaa tggtgattgc cctcacctct gcgggtaccc cgctcgcccg
    15421 ggaagcaacg ctggcaatta ccctcgacgt accggaagat actgacattt atatgcccat
    15481 ggtttctcga cttgcacagc tgaccgtgat agatgtgctg gcgacaggat ttactttgcg
    15541 acgcggtgca aaattcagag ataacttgaa gcgggtcaag gaagcgctga aggaatcgcg
    15601 ttttgataag cagttactta atttaagtga cgatcgctaa aaacgactgt cactgtccta
    15661 atcttatacg acatccgaat gagattaatt tatcgccatc gcggcgttat ttcattcgga
    15721 tttcatgttc aagcaacacc tggttgtttc agtcaacgga gtattacatg tccagaaggc
    15781 ttcgcagaac aaaaatcgtt accacgttag gcccagcaac agatcgcgat aataatcttg
    15841 aaaaagttat cgcggcgggt gccaacgttg tacgtatgaa cttttctcac ggctcgcctg
    15901 aagatcacaa aatgcgcgcg gataaagttc gtgagattgc cgcaaaactg gggcgtcatg
    15961 tggctattct gggtgacctc caggggccca aaatccgtgt atccaccttt aaagaaggca
    16021 aagttttcct caatattggg gataaattcc tgctcgacgc caacctgggt aaaggtgaag
    16081 gcgacaaaga aaaagtcggt atcgactaca aaggcctgcc tgctgacgtc gtgcctggtg
    16141 acatcctgct gctggacgat ggtcgcgtcc agttaaaagt actggaagtt cagggcatga
    16201 aagtgttcac cgaagtcacc gtcggtggtc ccctctccaa caataaaggt atcaacaaac
    16261 ttggcggcgg tttgtcggct gaagcgctga ccgaaaaaga caaagcagac attaagactg
    16321 cggcgttgat tggcgtagat tacctggctg tctccttccc acgctgtggc gaagatctga
    16381 actatgcccg tcgcctggca cgcgatgcag gatgtgatgc gaaaattgtt gccaaggttg
    16441 aacgtgcgga agccgtttgc agccaggatg caatggatga catcatcctc gcctctgacg
    16501 tggtaatggt tgcacgtggc gacctcggtg tggaaattgg cgacccggaa ctggtcggca
    16561 ttcagaaagc gttgatccgt cgtgcgcgtc agctaaaccg agcggtaatc acggcgaccc
    16621 agatgatgga gtcaatgatt actaacccga tgccgacgcg tgcagaagtc atggacgtag
    16681 caaacgccgt tctggatggt actgacgctg tgatgctgtc tgcagaaact gccgcagggc
    16741 agtatccgtc agaaactgtt gcagccatgg cgcgcgtttg cctgggtgcg gaaaaaatcc
    16801 cgagcatcaa cgtttctaaa caccgtctgg acgttcagtt cgacaatgtg gaagaagcta
    16861 ttgccatgtc agcaatgtac gcagctaacc acctgaaagg cgttacggcg attattacca
    16921 tgaccgaatc gggtcgtacc gcgctgatga cctcccgtat cagctctggt ctaccaattt
    16981 tcgccatgtc gcgccatgaa cgtacgctga acctgactgc tctctatcgt ggcgttacgc
    17041 cggtgcactt tgatagcgct aatgacggcg tagcagctgc cagcgaagcg gttaatctgc
    17101 tgcgcgataa aggttacttg atgtctggtg acctggtgat tgtcacccag ggcgacgtga
    17161 tgagtaccgt gggttctact aataccacgc gtattttaac ggtagagtaa tctcacctgc
    17221 cccggccgct gatggccggg gtatttagca acacataaat taggactact tattcaatcc
    17281 aatcataatc acagccccct gtaaaatgaa tacagggata gcctgaatgg agatctaaat
    17341 agcttgcaag acgtcgcata cccgcttcct ctgcaacact atgtccaata actgccatcg
    17401 gtaagccact atctttggag tattgtgtct gcacccatgt tttaattgaa tcgttaatcg
    17461 taatataata atcagcctga tattccatat attgtattgg atcacaaaaa cagcctgccc
    17521 caatgccaac agttctgact actctggctt tatctccata aaattgaatg ccaggaagat
    17581 tatatatttt caaatttttg gcaaaacgtt tggtaatgtt aatggcactg tcagggtcta
    17641 tagcatatac acagtacatc tcatcagaag caacaatatt tgtaacatcc agttctaact
    17701 gctgagccaa agccttagac attccgaacg taggctctcg atccatgaca tcatggcaac
    17761 gaatgattac caaattattt ttatgcaaaa actccttttt ttgctcaatt atttttaaat
    17821 aactgagttc accgcgagta taatccatta tttttctggc ccatgaaaac tcaagatcgt
    17881 caccctccag atcagcataa gaataaaatg taggctcatg tgtaataatg atattgacac
    17941 cttcaaaaac agcctttctt aaaacatcca ttgaggcaat ccagcaggtc ccaattttat
    18001 taataagtgt cgcctcagca ccaaaaataa ttttatcaac agttggttgc gaaacagcaa
    18061 ccattgaatt taaaaaacaa ttaagatccg cggctgtttt aatatgatgc attcaacatc
    18121 cttcacaggt ttcatcagca ataaaataat ttcaacattt ctgggaaacg ttttgcccat
    18181 gcagactcag agtgggtatc acctccccat aaa
//
LOCUS       DAZPDU010000082        17956 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZPDU010000082 DAZPDU010000000
VERSION     DAZPDU010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17956
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.083"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(227..1201)
                     /gene="cysB"
                     /locus_tag="ACHURS_002494"
     CDS             complement(227..1201)
                     /gene="cysB"
                     /locus_tag="ACHURS_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFR1238197.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(1411..4008)
                     /gene="topA"
                     /locus_tag="ACHURS_002495"
     CDS             complement(1411..4008)
                     /gene="topA"
                     /locus_tag="ACHURS_002495"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFR1238198.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTIGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKIGEIVTDRLEENFRELMNYDFTAQMENSLDLVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            4388..4639
                     /locus_tag="ACHURS_002496"
     CDS             4388..4639
                     /locus_tag="ACHURS_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFR1238199.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FIGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(4675..5724)
                     /gene="sohB"
                     /locus_tag="ACHURS_002497"
     CDS             complement(4675..5724)
                     /gene="sohB"
                     /locus_tag="ACHURS_002497"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFR1238200.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            5944..6702
                     /locus_tag="ACHURS_002498"
     CDS             5944..6702
                     /locus_tag="ACHURS_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFR1238201.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            6699..7289
                     /gene="cobO"
                     /locus_tag="ACHURS_002499"
     CDS             6699..7289
                     /gene="cobO"
                     /locus_tag="ACHURS_002499"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFR1238202.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(7329..8201)
                     /gene="rluB"
                     /locus_tag="ACHURS_002500"
     CDS             complement(7329..8201)
                     /gene="rluB"
                     /locus_tag="ACHURS_002500"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFR1238203.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(8414..9528)
                     /locus_tag="ACHURS_002501"
                     /pseudo
     CDS             complement(8414..9528)
                     /locus_tag="ACHURS_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
     gene            complement(9556..10176)
                     /locus_tag="ACHURS_002502"
     CDS             complement(9556..10176)
                     /locus_tag="ACHURS_002502"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFR1238204.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(10173..11054)
                     /gene="rnm"
                     /locus_tag="ACHURS_002503"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             complement(10173..11054)
                     /gene="rnm"
                     /locus_tag="ACHURS_002503"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFR1238205.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEVLVHRAVEMRVGTLAITD
                     HDNTAAIVPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGARRLAQGGAVTRGHFARFLVGCGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            11192..11236
                     /gene="trpL"
                     /locus_tag="ACHURS_002504"
     CDS             11192..11236
                     /gene="trpL"
                     /locus_tag="ACHURS_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFR1238206.1"
                     /translation="MKAIFVLKAWWRTS"
     misc_feature    11213..11306
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            11328..12890
                     /gene="trpE"
                     /locus_tag="ACHURS_002505"
     CDS             11328..12890
                     /gene="trpE"
                     /locus_tag="ACHURS_002505"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFR1238207.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKNTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSSYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSIPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            12890..14485
                     /gene="trpD"
                     /locus_tag="ACHURS_002506"
     CDS             12890..14485
                     /gene="trpD"
                     /locus_tag="ACHURS_002506"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFR1238208.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPILMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            14486..15847
                     /gene="trpCF"
                     /locus_tag="ACHURS_002507"
     CDS             14486..15847
                     /gene="trpCF"
                     /locus_tag="ACHURS_002507"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFR1238209.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIASIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLILVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            15859..17052
                     /gene="trpB"
                     /locus_tag="ACHURS_002508"
     CDS             15859..17052
                     /gene="trpB"
                     /locus_tag="ACHURS_002508"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFR1238210.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            17052..17858
                     /gene="trpA"
                     /locus_tag="ACHURS_002509"
     CDS             17052..17858
                     /gene="trpA"
                     /locus_tag="ACHURS_002509"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFR1238211.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPIEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISTPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKVATRS"
BASE COUNT         4317 a         4749 c         4488 g         4402 t
ORIGIN      
        1 cagcgaattt acgaatcgtc tccagtgaat aattcaatct tttgacctga aatagatatt
       61 tgaaaactgg agtttgcgag gcgggtaatt agacactact ttacaggtaa tttaccctat
      121 aaatatgagg gataaaaata ttgtgcacgg tatagaaata agtaacaaaa gagaatatat
      181 tccggcacct tcgctacata aaaggtgccg aaaataacgc aagaaattat ttttccggca
      241 gttttatatc tttaaacatg acctcaattt cttcattaga gcgcaatgcg acagccgcat
      301 caacgacatc acgcgttaaa tgcggtgcaa aacgctgaat gaaatcatac atataactgc
      361 gcaagaaagt actacggcga aaaccaattt tggttgtact gtggctgaag atatcgtgag
      421 catcaacacg cacaaggtcg ggatcggcga ccggatccac cgccatgctg gcaatgaccc
      481 ctacccccag ccctaaccgg acgtaagttt taatgacgtc agcatccgtt gccgtgaaga
      541 cgatacgcgg cgttaaccct gcgcggttaa aggcagtatc cagttctgaa cgtccggtaa
      601 agccgaaggt atatgtcacc aacggatatt gcgccagttc ttcaatggta atggcttttt
      661 tgcctgccag cgggtgatcc ggagtgacta caatagcccg attccagtgg tagcacggta
      721 acatcactaa atcttcatac agatgcagcg cttctgtggc gatagcgaaa tcagcattgc
      781 ctttagagac ggcatcagca atttgtgtcg gcgagccctg gtgcatatgc aaagaaacgc
      841 ggggataacg ctcaataaag cctttgataa cgtttggtaa cgcgtagcgt gcctgggtat
      901 gcgtggtggc gatatacagt gagcctttat ccggccaggt gtgctctccg gcaaccgatt
      961 ttatggcatc gactttcgac aggacttcgc gagcgatacg aattatttct tgccctgctg
     1021 gcgttacctg cgtcaggtgc ttgccgcttc gggaaaaaat ttgaatgcct agctcgtctt
     1081 ccagcattct gacttgttta ctgatcccgg gttgtgatgt gtaaagtcct tccgctgttg
     1141 atgagacatt caggttatga ttgaccacct caacaatata gcgaagttgt tgtaatttca
     1201 tgttaaacca tccacttaga cgccatcaga acatcgtttt ttcgtcacac ctggcgggtg
     1261 actaatacga tttaataata aaaaaggtgc taactataac cactatatca tttatagcct
     1321 tagtgtatag gtgtgaagaa aaaataatat gcaataaaaa agggccgctt tcgcgaccct
     1381 ttgtttataa aaacctgaca gaattaaagg ttattttttt ccttcaaccc atttgccatc
     1441 aacataaaat gctgaccagc cagtcgcctt tccgtctttt tccgaagaga catattgctg
     1501 tttggtttta cggctaaagc gaaccatggt cttattacct tccggatcct gctgtggcgc
     1561 atcggccaga taacgcagtt tttccggcag acggtcgcgg aagcgataaa gctcttccac
     1621 cagtggcgca cgcgtttcac gcgatttcgg gaaagtgttg gcagccagga aaacacccgc
     1681 agcgccgtca cgcagcacga aataagcatc tgatttttcg cacggcagct caggtaatgg
     1741 caccggatct tctttcggtg gtgccacttc gccgttacgt aaaatcttac gtgtgttttt
     1801 acactcttcg ttggtgcagg ccatgtattt accgaaacgc cccattttca ggtgcatttc
     1861 agagccacat ttttcacact caacgatcgg gccgtcataa cctttaatgc ggaattcgcc
     1921 ctcttcgatc tcgtaaccgt cgcaggttgg gttattacca cagacatgca acttacgttt
     1981 cggatcgatg agatagctgt ccatcgccgt gccgcatttc gggcaacgac gttttgcgcg
     2041 cagcgcgttg gtttcagcgt cttcgccttc cagtacgttc agcacttcgt tttccggcac
     2101 caggttaatg gtggttttgc aacgctcttt cggcggcagc gcataaccag aacagccaag
     2161 gaataccccg gtgctcgctg tgcgaatccc cattttgcga ccacaagtcg ggcagtcaat
     2221 gctggtcaga accatctggt tcgggcgcat accaccctct tccggatctt tttcagcttt
     2281 atctaactgc tgggtgaaat ccgagaagaa gtgatccagt acagctttcc actctgcttc
     2341 gtgatttgcc accaggtcga ggctgttttc catctgtgcg gtgaagtcgt agttcattaa
     2401 ctcgcggaaa ttttcttcaa ggcgatcggt gacgatttcg ccaatttttt ccgcatagaa
     2461 acgacgattt tctactcgca cgtagccacg atcctgaatg gtcgaaatga tcgacgcata
     2521 ggtagacgga cgaccgatac cgcgtttttc cagctcttta accagcgatg cttcactgaa
     2581 acgggctggc ggcttggtaa agtgctgggc tggtgtaagt tcaacgagcg tcagagcatc
     2641 gcctttatta actgctggta agatgcgatc ttcatcgcct ttacgcaacg caggcatcac
     2701 ttttgtccag ccatcaaaac gcaaaatacg accgcgtgct ttcaggcgga aatcgcccgc
     2761 accaatggtc agcgtcgtgg agtcatattt cgctggggtc atctggcagg ctacgaactg
     2821 acgccagatt aactggtaca gtttctgcgc atctgcttcc atatccttca gcgattccgc
     2881 catcacattg acgtcagaag gacgaatcgc ttcgtgcgct tcctgtgagt tttctttgct
     2941 ggcgtactga ttcggacttt ccggcagata tttcttacca aaattatcgc tgatataacc
     3001 acgaaccata tttaccgcgt cctgactcag gttagtggag tcggtacgca tgtaagtgat
     3061 atagcctgct tcatacaaac gctgcgccat catcatggtt tttttcacgc caaatccaag
     3121 acgggtgctg gcagcttgtt gcagcgtaga ggtaataaaa ggagcgccag gtttactggt
     3181 tgtcggtttg tcttcacgtt ccagcacgct gtagcgcgct ttttccagca gacttaccgc
     3241 agcctgagtt tgttctttgt tgaccggacg gaacggtttg tcgttctgat gagtcacctg
     3301 taacgccaac gcttcaccag atggcgtggt cgtgctggca tcgacttccc agaactcttc
     3361 cggcacgaac gctttaattt cacgctcacg ctcgaccacc aggcgaaccg ccaccgactg
     3421 cacacgaccg gcagacagac cacgagcgat ctttttccat agcagcggcg aaaccatata
     3481 ccccaccacg cggtccataa agcgacgcgc ctgctgggca ttaacacgat caatattcag
     3541 ctcacccggt ttgttaaatg cctggcggat cgcgttttta gtaatttcgt taaacaccac
     3601 tcggctatag cgcgcatcat cacccccaat cacttcccgc aggtgccatg caatggcttc
     3661 cccttcgcgg tcaaggtcgg ttgcgagata gatgtggtcg gctttttcag ccagttgttt
     3721 cagttcagag acgaccttct ctttaccagg caacacttca tagtgcgcct cccaattgtg
     3781 ccacgggtca acccccatac ggttgacgag agcgccacgt tcatcctttt taggcttttt
     3841 agccgtcttg gtggaggtag agtcggcact ctttttggca gctgagccac tggtcggcaa
     3901 atcgcggatg tgaccgacgc tggatttcac cacgtagtca ctacccagat acttgttgat
     3961 cgttttggct tttgccgggg actcaacgat gacaagagct ttacccatat tcaccttacc
     4021 taatttgatt cttccaggaa tgcgtcgcac gttgataaac cttccactgg caacgagtta
     4081 ccgatatgga ctcgttctct gcggatatca accccttgtc gtgactctgt tcacgaaaat
     4141 tcaggtgact gagttaacag gtcttggcct acaggcgcta tagcacgacg gaattttggt
     4201 cgaatgtcaa gcaaatctat tgccagattg acgaaagcgt cacactgtac ctgataaaat
     4261 ccgatacgca actttattag catgcaaaat ccaaagttgc cagtaaggcg ctcttgccac
     4321 cagcgtggta tatttgtctg caagccatgc ctggcgtaga ctaatgtcct tgtttaagga
     4381 gtaaataatg aacaaagaga cacaacccat cgaccgcgaa acgctgttga aagaggccaa
     4441 caaaatcatt cgtgagcatg aggatacgct cgcagggatt gaagccaccg gcgtcacgca
     4501 gcgtaatggc gtgctggtat ttatcggcga ctacttttta gatgaacaag gattgccaac
     4561 agccaaaagc accgcggtgt ttaatatgtt caaacacctg gcccatgtgc tttcagaaaa
     4621 atatcatctg gttgattaaa gcaaaacgcg aggtcttagc ctcgcgtttg tcttttacat
     4681 caatggcttt tgaccccgct gccaccagcg tagcaacaat cgatcggcgc tctctgccgc
     4741 gctgccggtg aatcggtcaa tgagtcgttt acgctgcata tagcgtacat taaccacttc
     4801 acggccttcc atcaggctaa gaataacttc atcactggtg ttgatttcat caaccaggcc
     4861 tttctctacc gcctgttgtc cgtaccagtg ttcacccgtt gccacctgtt caatatccag
     4921 agacggacgc atacgcttca caaaatcttt aaataactga tgcgtttcgt tcagctcttc
     4981 gcggaatttc tcccgccctt cttcggtatt ttcacccagc aaggtcagcg tgcgcttata
     5041 ctgcccggcg gtgtgcagtt cgatatcaat atctttgctt ttcaggaagc ggttaaagtt
     5101 gggcatttgc gccaccaccc caatggaacc cacaatagca aacggtgcgg aaacaatttt
     5161 gtccgccaca caggccatca tgtaaccgcc gctggcagcg actttgtcta ccgtaacagt
     5221 taaaggaatg tttttatcac gcagacgctg cagctgcgaa gccgccaacc cgtaaccatg
     5281 caccatgcca ccagggcttt ccagacggag cacaacctga tcctgcggtt tgaatgctgc
     5341 gagtaccgcc gttatctctt cacgtagcga gttcacttca tgggcgtcca tgctgccttt
     5401 aaaatccagc acccagacgc gaggtttact gtcagttgcc acctcgccca gtttggcttt
     5461 cgcttttgct gctttcgctt cttgcttgtg ctttttcttc tgcgctttgt gccactgttt
     5521 ttgctgatgt gagtccatca gcgccgcggc cagttcttct ttcatctcct tatactgttc
     5581 gctgagattg ttgacccgta actcgccacg ctggcgttta ttacgttgag caacattgac
     5641 aataatggcg gcaatcgccg caatcgctag cacaacggta acgattttcg ccaaaaacaa
     5701 accatattca gacaacaatt ccacgagtcc ccaccttggt taaacaacgc tttggctcac
     5761 agtttacaac aggcatgtta agccgtctcg cagagatgtt attgcagcga aagtatgaca
     5821 aagtatccat tttgcggtgt tatcgcccca ggctgattga aatgcccttc tgtttcaggc
     5881 ataaagcccc aaagtcataa agtacactgg cagcgcgaca gacaacgtcg ttgaggagta
     5941 gccatgcatt accagccaaa acaagattta ctcaatgatc gcattatcct ggtgacggga
     6001 gccagcgatg ggattggtcg tgaagccgcg atgacgtatg cacgctatgg tgcgacagtg
     6061 attctgttgg gccgtaatga agaaaaatta cgtcaggtag ccagccacat aaacgaagaa
     6121 actgggcgtc agccacagtg gtttattctc gatttgctga cctgcacgtc cgaaaattgc
     6181 caacaactgg cacagcgcat tgtcgttaat tatccgcgtc tggatggtgt tttgcataat
     6241 gccggattgc tcggcgatgt ttgcccaatg agcgaacaaa atccgcaggt ctggcaggac
     6301 gtcatgcagg tcaacgttaa tgccaccttt atgctcaccc aggcactgct tcctttatta
     6361 ctcaaatcgg acgccggttc actggtcttt acttcatcaa gcgttggacg tcagggacga
     6421 gccaactggg gtgcatatgc agcgtcgaaa tttgccaccg aagggatgat gcaggtactg
     6481 gccgatgaat atcagcagcg cctgcgcgtc aactgcatta acccaggcgg tacgcgcacc
     6541 gcaatgcgcg caagcgcctt cccgaccgaa gatccacaga aacttaaaac acccgctgat
     6601 atcatgccgc tctacctctg gctgatgggc gatgacagcc gccgtaaaac cggcatgacc
     6661 tttgacgccc aaccgggccg taaaccagga atttcccaat gagtgatgaa cgctaccaac
     6721 agcgtcagca gcgagtgaaa gaaaaagtag atgctcgtgt ggcccaggcc caggatgaac
     6781 gcggtattat catcgtcttt accggcaatg gaaaaggcaa aaccaccgcg gcatttggta
     6841 cggcaacacg cgcagttggt cacggaaaaa aagtgggcgt cgtgcagttt attaaaggca
     6901 cctggcctaa tggcgaacgc aatctgctgg agccacatgg cgttgagttt caggtgatgg
     6961 caacgggctt tacctgggat acacaaaacc gcgagtctga taccgccgcc tgccgcgaag
     7021 tctggcaaca tgcaaagcgg atgcttgctg attcctcact ggatatggtt ttgcttgatg
     7081 aactgacgta tatggtggcg tatgactatt tgccactgga agaagtggtg caggcgttaa
     7141 atgaacgtcc acatcaacag acggtgatta tcacgggtcg aggctgtcat cgggatattc
     7201 ttgaactggc ggatacggta agtgagctgc gccccatcaa acatgcgttt gatgccggtg
     7261 ttaaggcgca aatagggatt gattattaat ctgtaaaaat gccggatatg acgtccggca
     7321 tactttgatt aaccgttatt acgcccgcca gaacgacgac cgccgctcac ctgactgtga
     7381 cgtttcaccg cacgacgaat ctgattagcc ttcatgcgac gacggtcttt ttctacagcg
     7441 actttagagc tggtttctgg cggaagctcc accagttcgc gcagatagtt agtctgggcg
     7501 aggtccagct ccgtccagcc accgcgtggc agaccttttg gcagtgggat atcaccgtaa
     7561 cgaacacgga tcaggcggct cacctgcaca ccaaccgctt cccacagacg acgaacttca
     7621 cggttacggc cttcggtcag agtcacgttg taccactggt tgatcccttc gccgccgctg
     7681 aacttgatgg ttttaaaagc tgccggacca tcttccagtt gcacgccacg actcaaatca
     7741 cgcagtttcg cgtcgtcaac ctgaccaaat acacgcacgg cgtattcacg ttcaacttca
     7801 cggcttggat gcattaaacg gtttgccagt tcaccatcgg tggtgaacag cagcagacca
     7861 caggtgttaa cgtccagacg acctacggca atccagcgtg cgccacgcag ctttggcaga
     7921 cggtcaaaca ccgtcggacg cccttccgga tcgttacggg tacacaactc accttccggt
     7981 ttgtaatagg ccagaacgcg acaaatttgt tcagcggact cacgtaccga aatcagatga
     8041 ccatcgatac gaattttcag cccaggggta acttcaacgc gatcgccgag tttagcaatt
     8101 ttgccatcaa cactcacacg accggcttca ataatagatt cgatttcacg gcgggagccg
     8161 tggccagcgc gggccagcac tttctgtaat ttttcgctca tagagcttcc ttttggtgtc
     8221 gccttcccag gcgtctgata tgcttttgtg caacagtgag tcagaactta catctgactc
     8281 actgatagta aagtctgttt gaactcttca ggccttaagt aaacgactcg acgttttaac
     8341 tggcctggct ttgaggcgca acatactaca ctatctttat ggttaaagct tgtttcgatt
     8401 aacatcagct taattaccag ccaccaccgc ctccgccacc cgcgccgcca ccagcaccac
     8461 caccggaata acttcgcccc aacaaactgg ttttggcccc cagactcagg tgcatctgta
     8521 atgaaaacaa aacgccacta cgggcaattt ccggggcgct aacagccgct gtgagtgatg
     8581 gggtgatacg tgctacccat tctcttccaa ggtttgctga gactgcccaa cctagcaacg
     8641 cgtaatccag tttccgtgtt tcccctttcc gccgacgccc actgtgtgtt gctgcctctt
     8701 tacgcgcaag atagttaacg atagcttcac cgtgggcata acgttgctgc ccagtttgtg
     8761 taaactttgg cagaaaaata taccccatgc cagtgagata accggtcagg aaaacagcgc
     8821 taaaataacc cgctggcata taccagtaaa agaatacatc tccgacattg atgtagagag
     8881 aatagaaggc cacgcctgaa caaatcagtg gtaaacaatt cttccgtcct caaggatgcg
     8941 gccatttcga tatgtgtcgc cctcttctcc agtaaagaga tcgatttcgc taaatggatt
     9001 acccgcgata tcagggaact tgagtgaaaa tcgggtctta tagatttcaa acggccagtg
     9061 gttaccagtc acgttccaga ttaacagatc tgaatctccc tcacgcagga aagcattttt
     9121 aacctgataa tgaatttgat aatgatagtc gccttgaggc aagaaacgtt gtttatcgcc
     9181 cacgacaata ctcattaacc cgatatgcca gtcatgatgc cagggttcag gaataccatc
     9241 acgggtgacg ccaacaatat gatagttcag cagtgcagcg tccccatcag gctgcatcca
     9301 gagtcgtggt aaatcacgat aaaatccgtg tttaatctgc tgatttcgcg cctgaaaatg
     9361 aatattttct ttgatgtcga gcaccccatc cggttgcaga gtggcttcaa catcaaagag
     9421 cgaaatccct tcttcataag aagggatctc cgtgttttct gcataactta gagatgatac
     9481 aaatagccct gctatcaaca gcaaaatgca gcgaaacttc cctgccatag tcttccatcc
     9541 gtggaacgca atttgttata agaaaggctt cacatcacct acgccttcac gcaccacgac
     9601 cggcgtatca tcggtaagat caataaccgt tgtcggtttc tgcccgagat agccgccgtg
     9661 aataatcaaa tctacctgtt tttccagacg gtctttaatt tcttcgggat cggattcggt
     9721 aaattcgctg cctggcagca ttagcgaagt ggaaagcatc ggttcgccca gtgcttcaag
     9781 caacgcctgc gcaatcgggt tagaaggcac acgcataccg atggttttgc gtttttcctg
     9841 caacagacgg cgtggcactt ccttcgtccc cttcaggatg aaggtatagt tgcccggcgt
     9901 gttgtttttc attaaacgaa acgcaacgtt atcaacaaat gaataggtcg agagttcaga
     9961 aagatcgcga cacatcaggg taaagttgtg accgtccggc agctggcgaa tacgacaaat
    10021 acgctccatc gcgtttttat cttcaatttt acagccgagc gcatagccgg aatcagttgg
    10081 ataaacaatc accccgcctt tacgcacgat ctccaccgcc tggttgatca gacgttgctg
    10141 tgggttatca ggatgaatat aaaaaaactg gctcataatt ccctctctgt ggtgttctgc
    10201 ggctgttccc atagctgcca gacgccttca acgcctgcgg gcagccagag tttacgacct
    10261 agttcgatcc acgggcatgg ctgatgaaaa tcagatcctt gtgatgccca taaatgatgc
    10321 tgacgcgcaa gggccgccag ctgggtacgt tcattgggcg attgctggca ctgcgcgact
    10381 tccatcgcgt caccgtggtg ttcggcaaaa tgcgcaacca atcttttcag ccatttagca
    10441 ctaagattgt accgcccagg atgagctagc accgccttac cgccagaatg atgaatgaca
    10501 tcaatagctt gttctattgt acaccactgt ggcggaacgt atccggtttt cccgcgcgcc
    10561 agatattttt taaagacatc cgccattgaa ctggctttgc cgcaccccac aaggaaacgc
    10621 gcaaaatgac cgcgcgtcac cgcaccgccc tgcgccagtc gtcgcgcgcc ttccagtgcc
    10681 cctggtattt gcgctttttc aagtctttcg gcaatcagtt gcgcccgctg attgcgccgt
    10741 tctgtctgct gcgcgaggaa ctcacacatt agcggatgag taatatcaat attcagcccg
    10801 acaatatgaa tttcatgatt ttcccagacc gtggaaattt ccacgccggg aataagattc
    10861 agcgccagtc ccgaacgtga aatttcctct cttgctggca cgattgcagc tgtgttgtca
    10921 tggtcggtga tcgccagggt accgacgcgc atctcgactg cacggtgcac caatacttct
    10981 ggcgtcaggc agccatcgga agctgtggta tggctgtgca ggtcgtaaat cactgcgtaa
    11041 ttcgtgtcgc tcaaggcgca ctcccgttct ggataatgtt ttttgcgccg acatcataac
    11101 agttctggca aatattctga aatcagctgt tgacaattaa tcatcgaact agttaactag
    11161 tacgcaagtt cacgtaaaaa gggtatcgac aatgaaagca attttcgtac tgaaagcttg
    11221 gtggcgcact tcctgaaacg ggcagtgtat tcaccatgcg taaagcaatc agatacccag
    11281 cccgcctaat gagcgggctt ttttttgaac aaaattagag aataacaatg caaacacaaa
    11341 aaccgactct cgaactgcta acctgcgaag gcgcttatcg cgacaacccg actgcgcttt
    11401 ttcaccagtt gtgtgggaat cgtccggcaa cgctgctgct ggaatccgca gatatcgaca
    11461 gcaaagatga tttaaaaagc ctgctactgg tagacagtgc gctgcgcatt acagcattag
    11521 gtgacactgt cacaattcag gcactttccg gcaacggcga agccctgctg gcactactgg
    11581 ataacgccct gcctgcgggt gtggaaaatg aacaatcacc aaactgccgt gtgctgcgct
    11641 tcccccctgt cagtccactg ctggatgaag acgcccgctt atgctccctt tcggtttttg
    11701 acgctttccg tttattgcag aatctgttga atgtaccgaa ggaagaacga gaagcaatgt
    11761 tcttcggcgg cctgttctct tatgaccttg tggcgggatt tgaagattta ccgcaactgt
    11821 cagcggaaaa taactgccct gatttctgtt tttatctcgc tgaaacgctg atggtgattg
    11881 accatcagaa aaaaaacacc cgcattcagg ccagcctgtt tgctccgaat gaagaagaaa
    11941 aacaacgtct cactgctcgc ctgaacgatc ttcgccagca gctgaccgaa gccgcgccgc
    12001 cgctgccggt ggtttccgtg ccgcatatgc gttgtgaatg taatcagagc gatgaagagt
    12061 tcggtggcgt agtgcgtttg ttgcaaaaag cgattcgcgc tggagaaatt ttccaggtgg
    12121 tgccatctcg ccgtttctct ctgccctgcc cgtcaccgct ggcggcctat tacgtgctga
    12181 aaaagagtaa tcccagcccg tacatgtttt ttatgcagga taatgatttc accctgtttg
    12241 gcgcgtcgcc ggaaagttcg ctcaagtatg acgccaccag ccgccagatt gagatctacc
    12301 cgattgccgg aacacgtcca cgcggtcgtc gtgccgatgg ctcgctggac agagacctcg
    12361 acagccgcat cgaactggaa atgcgtaccg atcataaaga gctttctgaa catctgatgc
    12421 tggtggatct cgcccgtaat gatctggcac gcatttgcac ccccggcagc agctacgtcg
    12481 ccgatcttac caaagttgac cgttactctt acgtgatgca cctggtctcc cgcgtggtcg
    12541 gtgagctgcg ccacgatctc gacgccctgc acgcttaccg cgcctgtatg aatatgggga
    12601 cgttaagcgg tgcgccgaaa gtacgcgcca tgcagttaat tgccgaggcg gaaggtcgtc
    12661 gccgcggcag ctacggcggc gcggtaggtt atttcaccgc gcatggcgat ctcgacacct
    12721 gcattgtgat ccgctcggcg ctggtggaaa acggtatcgc caccgtgcaa gccggtgctg
    12781 gcgtagtcct tgattctatt ccgcagtcgg aagccgacga aacccgtaat aaagcccgcg
    12841 ctgtactgcg cgctattgcc accgcgcatc atgcacagga gactttctga tggctgacat
    12901 tctgctgctc gataatatcg actcttttac gtacaacctg gcagatcagt tgcgcagcaa
    12961 tgggcataat gtggtgattt accgcaacca tattccggcg cagaccttaa ttgaacgcct
    13021 ggcgacgatg agcaatccga tactgatgct ttctcctggc cccggtgtgc cgagcgaagc
    13081 cggttgtatg ccggaactcc tcacccgctt gcgtggcaag ctgcccatta ttggcatttg
    13141 cctcggacat caggcaattg tcgaagctta cgggggctat gtcggtcagg cgggcgaaat
    13201 tcttcacggt aaagcgtcga gcattgaaca tgacggtcag gcgatgtttg ccggattaac
    13261 aaacccgctg ccggtggcgc gttatcactc gctggttggc agtaacattc cggccggttt
    13321 aaccatcaac gcccatttta atggcatggt gatggcagta cgtcacgatg cggatcgcgt
    13381 ttgtggattc cagttccatc cggaatccat tctcaccacc cagggcgctc gcctgctgga
    13441 acaaacgctg gcctgggcgc agcagaaact agagccagcc aacacgctgc aaccgattct
    13501 ggaaaaactg tatcaggcgc agacgcttag ccaacaagaa agccaccagc tgttttcagc
    13561 ggtggtgcgt ggcgagctga agccggaaca actggcggcg gcgctggtga gcatgaaaat
    13621 tcgcggtgag cacccgaacg agatcgccgg ggcagcaacc gcgctactgg aaaacgcagc
    13681 gccgttcccg cgcccagatt atctgtttgc tgatatcgtc ggtactggcg gtgacggcag
    13741 caacagtatc aatatttcta ccgccagtgc gtttgtcgcc gcggcctgtg ggctgaaagt
    13801 ggcgaaacac ggcaaccgta gcgtctccag taaatctggt tcgtccgatc tgctggcggc
    13861 gttcggtatt aatcttgata tgaacgccga taaatcgcgc caggcgctgg atgagttagg
    13921 tgtttgtttc ctctttgcgc cgaagtatca caccggattc cgccacgcga tgccggttcg
    13981 ccagcaactg aaaacccgca ccctgttcaa tgtgctgggg ccattgatta acccggcgca
    14041 tccgccgctg gcgttaattg gtgtttatag tccggaactg gtgctgccga ttgccgaaac
    14101 cttgcgcgtg ctggggtatc aacgcgcggc ggtggtgcac agcggcggga tggatgaagt
    14161 ttcattacac gcgccgacaa tcgttgccga gctgcatgac ggcgaaatta agagctatca
    14221 attgaccgct gaagattttg gcctgactcc ctaccaccag gagcaactgg caggcggaac
    14281 accggaagaa aaccgtgaca ttttaacacg cttgttacaa ggtaaaggcg acgccgccca
    14341 tgaagcagcc gtcgctgcga acgtcgccat gttaatgcgc ctgcatggcc atgaagatct
    14401 gcaagccaat gcgcaaaccg ttcttgaggt actgcgcagt ggttccgctt acgacagagt
    14461 taccgcgctg gcggcacgag ggtaaatgat gcaaaccgtt ttagcgaaaa tcgtcgcaga
    14521 caaggcgatt tgggtagaaa cccgcaaaca gcagcaaccg ctggccagtt ttcagaatga
    14581 ggttcagccg agcacgcgac atttttatga tgcgctacag ggtgcacgca cggcgtttat
    14641 tctggagtgc aagaaagcgt cgccgtcaaa aggcgtgatc cgtgatgatt tcgatccggc
    14701 acgcattgcc tccatttata aacattacgc ttcggcaatt tcggtgctga ctgatgagaa
    14761 atattttcag gggagctttg atttcctccc catcgtcagc caaatcgccc cgcagccgat
    14821 tttatgtaaa gacttcatta tcgaccctta ccagatctat ctggcgcgct attaccaggc
    14881 cgatgcctgc ttattaatgc tttcagtact ggatgacgac caatatcgcc agcttgcagc
    14941 cgtcgctcac agtctggaga tgggggtgct gaccgaagtc agtaatgaag aggaactgga
    15001 gcgcgccatt gcattggggg caaaggtcgt tggcatcaac aaccgcgatc tgcgtgattt
    15061 gtcgattgat ctcaaccgta cccgcgagct tgcgccgaaa ctggggcaca acgtgacggt
    15121 aatcagcgaa tccggcatca atacttacgc tcaggtgcgc gagttaagcc acttcgctaa
    15181 cggttttctg attggttcgg cgttgatggc ccatgacgat ttgcacgccg ccgtgcgccg
    15241 ggtgttgctg ggtgagaata aagtatgtgg tctgacgcgt gggcaagatg ctaaagcagc
    15301 ttatgacgcg ggcgcgattt acggtgggtt gattttggtt gcgacatcac cgcgttgcgt
    15361 caacgttgaa caggcgcagg aagtgatggc tgcggcaccg ttgcagtatg ttggcgtgtt
    15421 ccgcaatcac gatattgccg atgtggtgga caaagctaag gtgttatcgc tggcggcagt
    15481 gcaactgcat ggtaatgaag atcagctgta tatcgatacg ctgcgtgaag ctctgccagc
    15541 acacgttgcc atctggaaag cattaagcgt cggtgaaacc ctgcccgccc gcgagcttca
    15601 gcacgttgat aaatatgttt tagacaacgg ccagggtgga agcgggcaac gtttcgactg
    15661 gtcactatta aatggtcaat cgcttggcaa cgttctgctg gcggggggct taggcgcaga
    15721 taactgcgtg gaagcggcac aaaccggctg cgccggactt gattttaatt ctgctgtaga
    15781 gtcgcaaccg ggcatcaaag acgcacgtct tttggcctcg gttttccaga cgctgcgcgc
    15841 atattaagga aaggaacaat gacaacatta cttaacccct attttggtga gtttggcggc
    15901 atgtacgtgc cacaaatcct gatgcctgct ctgcgccagc tggaagaagc ttttgtcagc
    15961 gcgcaaaaag atcctgaatt tcaggcccag ttcaacgacc tactgaaaaa ctatgccggg
    16021 cgtccaaccg cactgaccaa atgccagaac attacggctg gcacgaacac cacgctgtat
    16081 ctgaagcgcg aagatttgct gcacggcggc gcgcataaaa ctaaccaggt gctcggtcag
    16141 gctttactgg cgaagcggat gggtaaaacc gaaatcatcg ccgaaaccgg tgccggtcag
    16201 catggcgtgg cgtcggccct tgccagcgcc ctgctcggcc tgaaatgccg tatttatatg
    16261 ggtgccaaag acgttgaacg ccagtcgcct aacgtttttc gtatgcgctt aatgggtgcg
    16321 gaagtgatcc cggtgcatag cggttccgcg acgctgaaag atgcctgtaa cgaggcgctg
    16381 cgcgactggt ccggtagtta cgaaaccgcg cactatatgc tgggcaccgc agctggcccg
    16441 catccttatc cgaccattgt gcgtgagttt cagcggatga ttggcgaaga aaccaaagcg
    16501 cagattctgg aaagagaagg tcgcctgccg gatgccgtta tcgcctgtgt tggcggcggt
    16561 tcgaatgcca tcggcatgtt tgctgatttc atcaatgaaa ccaacgtcgg cctgattggt
    16621 gtggagccag gtggtcacgg tatcgaaact ggcgagcacg gcgcaccgct aaaacatggt
    16681 cgcgtgggta tctatttcgg tatgaaagcg ccgatgatgc aaaccgaaga cgggcagatt
    16741 gaagaatctt actccatctc cgccggattg gatttcccgt ctgtcggccc acaacacgcg
    16801 tatcttaaca gcactggacg cgctgattac gtgtctatta ccgatgatga agcccttgaa
    16861 gccttcaaga cgctgtgcct acacgaaggg atcatcccgg cgctggaatc ctcccacgcc
    16921 ctggcccatg cgctgaaaat gatgcgcgaa aacccggaaa aagagcagct actggtggtt
    16981 aacctttccg gtcgcggcga taaagacatc ttcaccgttc acgatatttt gaaagcacga
    17041 ggggaaatct gatggaacgc tacgaatctc tgtttgccca gttgaaggag cgcaaagaag
    17101 gcgcattcgt tcctttcgtc acgctcgggg atccgggcat tgagcagtca ttgaaaatta
    17161 tcgatacgct aattgaagcc ggtgctgacg cgctggagtt aggcatcccc ttctccgacc
    17221 cactggcaga tggcccgacg attcaaaacg ccactctgcg cgcctttgcg gcaggtgtga
    17281 ctccggcaca atgttttgaa atgctggcac tgattcgcca gaaacacccg accattccca
    17341 ttggcctgtt gatgtatgcc aatctggtgt ttaacaaagg cattgatgag ttttatgccc
    17401 agtgcgagaa agtcggcgtc gattcggtgc tggttgccga tgtgccaatt gaagagtccg
    17461 cgcccttccg ccaggccgcg ttgcgccata atgtcgcacc tatcttcatc tgcccgccaa
    17521 atgccgatga cgacctgctg cgccagatag cctcttacgg tcgtggttac acctatttgc
    17581 tgtcacgagc gggcgtgacc ggcgcagaaa accgcgccgc gttacccctc aatcatctgg
    17641 ttgcgaagct gaaagagtac aacgctgcac ctccattgca ggggtttggt atttccaccc
    17701 cggatcaggt aaaagcagcg attgatgcag gagctgcggg cgcgatttct ggttcagcca
    17761 ttgttaaaat catcgagcaa catattaatg agccagagaa aatgctggcg gcactgaaag
    17821 tttttgtaca accgatgaaa gtggcgacgc gcagttaatc ccccagccgc cagttccgct
    17881 ggcggcattt taactttctt taacgaagcc ggaaaaatcc taaattcatt taatatttat
    17941 ctttttaccg tttcgc
//
LOCUS       DAZPDU010000083        17891 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZPDU010000083 DAZPDU010000000
VERSION     DAZPDU010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17891
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.084"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            175..853
                     /locus_tag="ACHURS_002510"
                     /pseudo
     CDS             175..853
                     /locus_tag="ACHURS_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            897..5747
                     /locus_tag="ACHURS_002511"
     CDS             897..5747
                     /locus_tag="ACHURS_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFR1238212.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDSWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKTLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTASIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIEENGAGINYVRTN
                     DNDLAFNDASASGVGATAVGYNAVASGASSVAIGQNSSSTVDTGIALGSSSVSSRVIA
                     KGSRDTSVTENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTAEDSLAVGEDSLAMGAKTVVNGNAGIGIGLNTLVLADAI
                     NGIAIGSNARANHANSIAMGNGSQTTRGAQTGYTAYNMDAPQNSVGEFSVGSEDGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDERVAQNTQSITNLNNQVTNLDTRVTNIENGIGDIV
                     TTGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVAEEENTISVGSS
                     TNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGSTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            6115..7770
                     /gene="lldP"
                     /locus_tag="ACHURS_002512"
     CDS             6115..7770
                     /gene="lldP"
                     /locus_tag="ACHURS_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFR1238213.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASKIDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7770..8546
                     /gene="lldR"
                     /locus_tag="ACHURS_002513"
     CDS             7770..8546
                     /gene="lldR"
                     /locus_tag="ACHURS_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFR1238214.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8543..9733
                     /gene="lldD"
                     /locus_tag="ACHURS_002514"
     CDS             8543..9733
                     /gene="lldD"
                     /locus_tag="ACHURS_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFR1238215.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9917..10390
                     /gene="trmL"
                     /locus_tag="ACHURS_002515"
     CDS             9917..10390
                     /gene="trmL"
                     /locus_tag="ACHURS_002515"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFR1238216.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10443..11264)
                     /gene="cysE"
                     /locus_tag="ACHURS_002516"
     CDS             complement(10443..11264)
                     /gene="cysE"
                     /locus_tag="ACHURS_002516"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFR1238217.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11344..12363)
                     /gene="gpsA"
                     /locus_tag="ACHURS_002517"
     CDS             complement(11344..12363)
                     /gene="gpsA"
                     /locus_tag="ACHURS_002517"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFR1238218.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12363..12830)
                     /gene="secB"
                     /locus_tag="ACHURS_002518"
     CDS             complement(12363..12830)
                     /gene="secB"
                     /locus_tag="ACHURS_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFR1238219.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSVVSRGTFPQLNLAPINFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12893..13144)
                     /gene="grxC"
                     /locus_tag="ACHURS_002519"
     CDS             complement(12893..13144)
                     /gene="grxC"
                     /locus_tag="ACHURS_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFR1238220.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13286..13717)
                     /locus_tag="ACHURS_002520"
     CDS             complement(13286..13717)
                     /locus_tag="ACHURS_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFR1238221.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13962..15506
                     /gene="gpmM"
                     /locus_tag="ACHURS_002521"
                     /gene_synonym="pgmI"
     CDS             13962..15506
                     /gene="gpmM"
                     /locus_tag="ACHURS_002521"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFR1238222.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15540..16799
                     /gene="envC"
                     /locus_tag="ACHURS_002522"
     CDS             15540..16799
                     /gene="envC"
                     /locus_tag="ACHURS_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFR1238223.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIATKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16803..17762
                     /locus_tag="ACHURS_002523"
     CDS             16803..17762
                     /locus_tag="ACHURS_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFR1238224.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPNNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASVN"
     gene            complement(17749..>17891)
                     /locus_tag="ACHURS_002524"
     CDS             complement(17749..>17891)
                     /locus_tag="ACHURS_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFR1238225.1"
                     /translation="MYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHHRITRSAFN
                     LR"
BASE COUNT         4493 a         4629 c         4676 g         4093 t
ORIGIN      
        1 atacaaacac agacatcttt tgaaacgaat gatacatttt tatgatgaat gcttaaaaca
       61 atatttaatc gacacacatc actgtcatta atgtaaagca acatagatca ccaataatac
      121 aatggacaac tctaccggca ttgatcgctg gtcggaggaa ataaggattt ttacatgaac
      181 ttgaagaaga tttttttcag tgctgtcact gtttcagttc tctgcgcatt aacgggatgt
      241 gactacattg aagaaggcaa gccagaaagt agtttattga aacaacaaga agaacattaa
      301 aattgatcta cttgaaaaac aacaagcaca gctaaagagt caactggaaa ctattcaaaa
      361 acaacaaact ggcataataa gcagtacaaa gactttaact cacgtaatta aatccgttaa
      421 agatcaacaa aacactttta tttttaccga gtttaaccca gcaaaaacga agtatttcat
      481 cctgaacaat ggttcggtgg ctttagcggg tcgagtgtta tccattgacg ccacagagaa
      541 tggcagtgtt attcatattt cactggtcaa cttattaagt acacctatct caaatattgg
      601 ttttaatgcg acatggggtg gtgaaaaacc tgtcgatgcc aaagagtttg cccggtggca
      661 acaattactt ttcaacacat caatgaaatc cacattgaaa ttattaccag gtcaatggca
      721 agacattaat ttgaccctga agggtgtatc gcccaataac ctgggatatc tgaagttagc
      781 catcaacatg gaaaatattc agttcgacaa tcttccctct gctgaaaatc ggcagaaaag
      841 aagcaaaaaa taaatgcagt tagtacgctt ataacaagga atagcgagca tcattaatga
      901 acaaaatatt taaagttatc tggaaccctg cgacagggaa ttatactgtt accagcgaaa
      961 cggcaaaaag ccgtggcaag aaatctgggc gcagtaagct gttaatttct gcgctggttg
     1021 cgggtggaat gttgtcgtcg tttggggcat tggcgaatgc cgggaatgac aacggccagg
     1081 gtgttgatta cggtagtgga tcagctggcg acagctgggt tgctataggc aaaggggcga
     1141 aagcaaatac ttttatgaac accagtggtt ccagtactgc tgtgggttat gacgctatag
     1201 ctgaaggcca atatagctct gccatcgggt caaaaaccca tgcgattggt ggtgcatcaa
     1261 tggcctttgg ggttagtgca atatcagaag gcgatagaag tatagcactg ggtgcctctt
     1321 cgtattcatt gggccaatac tcaatggccc tcggccgtta ttcaaaagca ttgggtaaat
     1381 tgtctattgc tatgggggac tcttccaaag cggaaggagc aaacgccatt gccctgggaa
     1441 atgccactaa agctactgag attatgagta ttgctcttgg cgacaccgcc aatgcgtcaa
     1501 aagcgtattc aatggcgctg ggagcaagta gcgtcgcatc tgaagaaaac gcaattgccc
     1561 tggggcgtag cagtgtagct agcggtactg acagcctcgc atttggcaga caatcacttg
     1621 ccagcgcagc gaacgctatt gcgataggtg ctgagaccga agccgctgaa aatgcaactg
     1681 ctattggcaa taatgcgaag gcaaaaggga ctaatagcat ggcaatgggg ttcggaagcc
     1741 ttgccgataa agtcaatact atcgcattag gaaatggcag ccaggctctg gcagataatg
     1801 caatcgccat aggccagggc aacaaagctg atggcgtgga tgccatcgct ctgggtaatg
     1861 gtagccagtc gagaggctta aacaccattg ccttaggcac agccagtaat gcaactggtg
     1921 ataagagtct tgcgcttggt agtaatagca gtgccaacgg tattaactct gtcgcgctgg
     1981 gcgcagattc cattgcggat ttagacaata ccgtctctgt cggcaatagt tcattaaaac
     2041 gcaagatcgt taatgtgaaa aatggcgcga tcaagtctga cagttacgat gccattaatg
     2101 gttcacagct ttatgccatt agcgactcgg tagcaaaaac gcttggagga ggggctgcag
     2161 tagatgttga tgacggtact gttacagcac caacctacaa tttaaaaaat ggtagcaaaa
     2221 ataacgtagg ggctgcgctc gctgtacttg atgaaaacac cctgcaatgg gaccaaacca
     2281 aaggcaaata cagcgctgct catggtacta gtagcccaac tgccagcgta atcaccgatg
     2341 ttgcggatgg cacgatttca gcctccagta aggatgcggt taacggttcc caactgaaag
     2401 ctaccaatga cgatgtcgaa gccaacaccg ccaatatcgc tactaatacc agcaacattg
     2461 ccacgaatac agcaagtatt gccaccaata ccaccaatat caccaacctg acggattccg
     2521 ttggtgacct tcaggctgat gccctgctct ggaacgaaac taaaaaggca ttcagtgcag
     2581 ctcacggcca ggataccacc agcaaaatca ccaacgttaa agatgccgac ctgacggctg
     2641 acagcactga tgctgttaac ggctctcagc tgaaaaccac caacgatgct gtggcgacga
     2701 ataccaccaa tatcgccaat aacacttcca atattgccac taacaccacc aacatctcta
     2761 atctgactga gacggtgact aatcttggtg aggatgcgct gaaatgggat aaggacaatg
     2821 gtgtattcac ggcagctcat ggcaccgaga ccaccagcaa aatcaccaac gttaaagatg
     2881 gcgacctgac gactggcagc accgatgccg ttaacggctc tcagctgaaa accaccaacg
     2941 atgccgtggc gacgaatacc accaatatcg ccactaacac caccaacatc tctaatctga
     3001 ctgagacggt gactaatctt ggtgaggatg cgctgaaatg ggataaggac aatggtgtct
     3061 tcactgcagc tcatggcaac aataccgcca gcaaaatcac caatatcctg gacggcacag
     3121 tcactgcaac cagttccgat gccattaacg gtagccagct ttatgactta agcagcaata
     3181 tcgccaccta cttcggcggc aatgcttctg tgaatactga cggtgtgttt accggtccaa
     3241 cctacaaaat cggtgaaaca aattattata acgtcggcga tgcactggct gcgattaact
     3301 cctcatttag cacgtctctc ggcgatgctc tgctttggga tgccaccgca ggtaaattca
     3361 gtgccaaaca cggtactaat ggtgacgcaa gcgtgatcac tgatgtcgca gatggtgaaa
     3421 tttcagactc cagttctgac gcagtaaacg gctcacaact ccacggcgtg agcagttatg
     3481 ttgttgatgc gctggggggt ggtgccgaag tcaatgcaga cggcaccatc actgcgccga
     3541 cgtacaccat tgctaatgct gattacgata atgtcggtga tgccctgaat gctatcgata
     3601 ccactcttga cgacgctctg ctctgggatg cggacgccgg tgaaaatggt gcatttagcg
     3661 ccgctcacgg aaaagataaa actgccagtg taatcactaa cgtcgctaac ggtgcaatct
     3721 ctgctgccag cagcgacgcg attaacggct cacaactcta taccaccaat aagtacatcg
     3781 ctgatgcgct gggtggtgac gcagaagtca acgctgacgg caccatcacc gcaccgactt
     3841 acaccattgc gaacgccgag tacaacaacg tcggtgacgc cctggatgcg cttgatgata
     3901 acgccctgct gtgggatgag actgccaatg gcggtgctgg agcctacaat gccagccatg
     3961 acggtaaagc cagcatcatc actaatgtcg ctaatggcag tattagtgag gacagtaccg
     4021 atgcagtgaa cggttctcag ttgaatgcga cgaatatgat gattgagcag aacacccaaa
     4081 ttatcaatca gctcgctggt aacaccgacg caacctatat cgaagaaaat ggcgctggta
     4141 tcaactacgt tcgtactaac gacaacgatt tagcctttaa cgatgcaagc gcctctggtg
     4201 tcggcgctac agctgtaggt tataacgctg tcgcgtctgg tgccagcagc gtagccattg
     4261 gtcagaacag cagcagcacc gttgataccg gtattgcgct gggtagcagc tccgtttcca
     4321 gccgtgtgat tgccaaaggt tctcgtgaca ctagcgtaac ggaaaatggc gtggtgattg
     4381 gttatgacac cactgacggc gaactgctag gtgcattgtc aattggtgat gacggtaaat
     4441 accgccaaat catcaacgta gctgatggtt cagaagccca tgacgccgtt acggttcgcc
     4501 agttgcagaa cgctattgga gcggtcgcca ctacgccaac caagtacttt cacgccaact
     4561 caacggcaga agactcactg gccgttggtg aagactcgct ggcaatgggt gcgaaaactg
     4621 tcgttaatgg taatgcaggt attggtatcg gtttgaacac tctggttcta gctgatgcga
     4681 tcaacggcat tgctatcggc agtaacgcac gcgcaaacca tgcaaacagt atcgcaatgg
     4741 gtaacggttc tcagaccacc cgtggtgcac agactggcta caccgcctac aacatggacg
     4801 caccgcagaa ctctgtaggt gagttttctg tcggtagtga agacggtcaa cgtcagatca
     4861 caaacgtcgc agctggttca gcggataccg atgcggttaa cgtgggtcag ttgaaagtca
     4921 ctgatgagcg cgtagcgcaa aatacccaga gcattactaa cctgaacaat caggtcacta
     4981 atctggatac tcgcgttact aatatcgaaa acggtattgg cgacattgtc accaccggta
     5041 gcaccaagta cttcaagacc aacaccgatg gcgtagatgc caacgcccag ggtaaagata
     5101 gcgttgctat tggttctggt tccattgctg ccgctgacaa cagcgtcgca ctgggtaccg
     5161 gttccgttgc agaggaagaa aatacaatct ctgtaggttc ttctactaac caacgccgta
     5221 tcaccaacgt agctgccggt gttaatgcca ccgatgcggt taacgtgtcg cagctgaaat
     5281 cttctgaagc aggcggcgtg cgctacgaca ccaaagctga tggttctatc gactatagca
     5341 atatcaccct cggtggtggc aacggcagta cgactcgtat cagcaacgtc tccgctggcg
     5401 tcaacaacaa cgacgcggtg aactacgcgc agttgaagca aagcgtgcag gaaacgaagc
     5461 aatacaccga tcagcggatg gttgagatgg ataacaaact gtctaaaact gaaagcaagt
     5521 tgagcggtgg tatcgcttct gcaatggcaa tgaccggtct gccgcaggct tatacaccgg
     5581 gtgccagcat ggcttctatt ggtggcggta cttacaacgg tgaatcggca gttgctttag
     5641 gtgtatcgat ggtgagcgcc aatggtcgtt gggtctacaa attacaaggt agtaccaata
     5701 gccagggtga atactccgcc gcactcggtg ccggtattca gtggtaatga tccattaagt
     5761 tagtgtgact aagggcaaac gccaaattgc ctgatgcgtt acgcgcatca ggcaagataa
     5821 ggcgccccgg gtcaacaatc taagggcaat tctctgatga ggattgccct tttctttacc
     5881 agacatctcc ccccacaaga attggcccta ccaattcttc gcttatctga cctctggttc
     5941 acaattcccc aattaaaact cacatcaata ttgccaatac ataacattta gttaaccatt
     6001 cattgtcatt atccctacac aacacaattg gcagtgccac ttttacacaa cgtgtgacaa
     6061 ggagatgagc aacagactca ttacacgatg tgcgtggact ccaggagacc tgcaatgaat
     6121 ctctggcaac aaaactacga tcccgccggg aatatctggc tttccagtct gatagcatcg
     6181 cttcccatcc tgtttttctt ctttgcgctg attaagctca aactgaaagg atacgtcgcc
     6241 gcctcatgga cggtagcaat cgcccttgcc gtggctttgc tgttctataa aatgccggtc
     6301 gctaacgcgc tggcctcggt ggtttatggc ttcttctatg gtttgtggcc catcgcgtgg
     6361 atcattattg cagcagtgtt cgtctataag atctcggtga aaaccgggca gtttgacatc
     6421 attcgctcgt ctattctttc gataacccct gaccagcgtc tgcaaatgct gatcgtcggt
     6481 ttctgtttcg gcgctttcct tgaaggagcc gcaggctttg gcgcaccggt agcaattacc
     6541 gccgcattgc tggtcggcct gggttttaaa ccgctgtacg ccgccggact gtgcctgatt
     6601 gttaacaccg cgccagtggc atttggcgcg atgggcattc caatcctggt tgccggacaa
     6661 gtaacaggca tcgacagctt tgagattggc cagatggtgg ggcggcagct accgtttatg
     6721 accattatcg tgctgttctg gatcatggcg attatggacg gctggcgcgg tatcaaggag
     6781 acgtggcctg cggtcgtggt tgcgggcggc tcgtttgcca tcgctcagta ccttagctct
     6841 aacttcattg ggccggagct gccggacatt atctcttcgc tggtatcact gctctgcctg
     6901 acgctgttcc tcaaacgctg gcagccagtg cgcgtattcc gttttggtga tttgggggcg
     6961 tcaaagattg atatgacgct ggcccacacc ggttacactg cgggtcaggt gttacgtgcc
     7021 tggacaccgt tcctgttcct gacagctacc gtaacactgt ggagcatccc gccgtttaaa
     7081 gccctgttcg catcgggtgg tgcgctgtat gagtgggtga tcaacattcc ggtgccgtac
     7141 ctcgataaac tggttgcccg tatgccacca gtggtcagcg aggctacagc ctatgccgcc
     7201 gtgtttaagt ttgactggtt ctctgccacc ggcaccgcca ttctgtttgc tgcactgctc
     7261 tcgattgtct ggctgaagat gaaaccgtct gacgctatca gcaccttcgg cagcacgctg
     7321 aaagaactgg ctctacccat ctactccatc ggcatggtgc tggcgttcgc ctttatctcg
     7381 aactattccg gactgtcatc aacactggca ctggcactgg cgcacaccgg tcatgcattc
     7441 accttcttct cgccgttcct cggctggctg ggggtattcc tgaccgggtc ggatacctca
     7501 tctaacgccc tgttcgccgc gctgcaagcc accgcagcac aacaaattgg cgtctctgat
     7561 ctgttgctgg ttgccgccaa taccaccggt ggcgtcaccg gtaagatgat ctccccgcaa
     7621 tctatcgcta tcgcctgtgc ggcggtaggc ctggtgggca aagagtctga tttgttccgc
     7681 tttactgtca aacacagcct gatcttcacc tgtatggtgg gcgtgatcac cacgcttcag
     7741 gcttatgtct taacgtggat gattccttaa tgattgtttt acccagacgc ctgtcagacg
     7801 aggttgccga tcgtgtgcgg gcgctgattg atgaaaaaaa cctggaagcg ggcatgaagt
     7861 tgcccgctga gcgccaactg gcgatgcaac tcggcgtatc acgtaattca ctgcgcgagg
     7921 cgctggcaaa actggtgagc gaaggcgtgc tgctcagtcg acgcggcggc gggacgttta
     7981 ttcgctggcg tcatgacaca tggtcggagc aaaacatcgt ccagccgcta aaaacgctga
     8041 tggccgatga tccggattac agtttcgata ttctggaagc ccgctacgcc attgaagcca
     8101 gcaccgcatg gcatgcggca atgcgcgcca cacctggcga caaagaaaag attcagcttt
     8161 gctttgaagc aacgctaagt gaagacccgg atatcgcctc acaagcggac gttcgttttc
     8221 atctggcgat tgccgaagcc tcacataaca tcgtgctgct gcaaaccatg cgcggtttct
     8281 tcgatgtcct gcaatcctca gtgaagcata gccgtcagcg gatgtatctg gtgccaccgg
     8341 ttttttcaca actgaccgaa caacatcagg ctgtcattga cgccattttt gctggtgatg
     8401 ccgacggggc gcgtaaagca atgatggcgc accttagttt tgttcacacc accatgaaac
     8461 gattcgatga agatcaggct cgccacgcac ggattacccg cctgcccggt gagcataatg
     8521 agcattcgag ggagaaaaac gcatgattat ttccgcagcc agcgattatc gcgccgcagc
     8581 gcaacgcatt ctgccgccgt tcctgttcca ctatatggat ggtggtgcat attctgaata
     8641 cacgctgcgc cgcaacgtgg aagatttgtc agaagtggca ctgcgccagc gtattctgaa
     8701 aaacatgtcc gacttaagcc tggaaacgac gctgtttaat gagaaattgt cgatgccggt
     8761 agcactggca ccggtgggtt tgtgtggcat gtatgcgcgt cgtggcgaag ttcaggcagc
     8821 caaagcggcg gacgcgcatg gcattccgtt tactctctcg acggtttccg tttgcccgat
     8881 tgaagaagtc gcgccagcca tcaagcgccc aatgtggttc cagctttatg tactgcgcga
     8941 tcgcggcttt atgcgtaacg cgctggagcg agcaaaagca gcgggttgtt cgacgctggt
     9001 tttcaccgtg gatatgccga caccgggcgc acgctaccgt gatgcgcatt caggtatgag
     9061 cggcccaaat gcggcaatgc gccgctactt gcaggcggtg acgcatccgc aatgggcgtg
     9121 ggatgtgggc ctgaacggtc gtccacatga tttaggtaat atctcagctt atctcggcaa
     9181 accgaccgga ctggaagatt acatcggctg gctggggaat aacttcgatc cgtccatctc
     9241 atggaaagac cttgagtgga tccgcgattt ctgggatggc ccgatggtga tcaaagggat
     9301 cctcgatccg gaagatgcgc gcgatgcagt acgttttggt gctgatggaa ttgtggtttc
     9361 taaccacggt ggccgccagc tggacggtgt actctcttcc gctcgtgcgt tgcccgctat
     9421 tgcagatgcg gtgaaaggtg atatcgccat tctggcggat agcggaattc gtaacgggct
     9481 tgatgtcgtg cgtatgattg cgctcggtgc cgacaccgta ctgctgggtc gtgctttcct
     9541 gtatgcgctg gcaacagcgg gccaggcggg tgtagctaac ctgctaaatc tgatcgaaaa
     9601 agagatgaaa gtggcgatga cgctgactgg cgcgaaatcg atcagtgaaa ttacgcaaga
     9661 ttcgctggtg caggggctgg gtaaagagtt gcctacggca ctggctccga tggcgaaagg
     9721 gaatgcggca tagtcgtttg cgccccctca ccctcaggga gaggggaccg ttcggcgctg
     9781 tatgtactcc ctcactctga aacgacaccg cactcttttt ttctccctcg cccctccggg
     9841 gagggccggg gtgaggggaa aaggccgcac ggttcaccaa ttttctgcta tcctgccccc
     9901 gcactaaggg ggcagtatgc taaacatcgt actttacgaa ccagaaattc cgccaaatac
     9961 tggcaacatc atccgtcttt gcgctaatac cggctttcgt ctgcatatca tcgaaccgat
    10021 gggatttgcc tgggacgata agcgcctgcg ccgcgcgggg ctggactatc acgagtttac
    10081 cgccgttacg cgtcatcatg actatcgcgc gttcctcgaa gcagaaaatc cccagcgcct
    10141 gtttgccctc accacgaaag gtacgcctgc tcacagcgcc gtaagctatc aggatggcga
    10201 ttatctgatg ttcggcccgg aaacacgcgg cctgccagcg agcattcttg atgccctgcc
    10261 cgctgaacaa aaaattcgca ttccgatggt gccggacagc cgcagcatga atctgtccaa
    10321 tgcggtgtcg gtagtggtgt atgaagcctg gcggcagctg ggatatccgg gagcgttgtt
    10381 gagagattag ttactgtatg ccggatagat gattacatcg catccggcac gatcacagga
    10441 cattagatcc catccccata ctcaaatgta tggttaatac cgttgaaatg ctggtccata
    10501 tccattgatg gcttatcgct gtctggttta ccgacaatac gagccggaac gccagcggcg
    10561 gttgtatgcg gcggcaccgg ttgcagcacc acggaacctg cgccaatctt cgcgccgcga
    10621 ccaacttcaa tattgccgag gattttcgcg cccgcgccaa tcatcacacc ttcacgaatt
    10681 ttcgggtgac ggtcaccacc agatttaccc gtaccgccaa gcgtcacaga ttgcagaatc
    10741 gatacgtcgt tttcaattac cgccgtttca cccacaacga tgcccgtggc gtggtcgagc
    10801 ataatgccac ggccaatttt tgctgccggg tgaatatcga cctggaacgt cacagaaacc
    10861 tggttttgca gaaagattgc caatgcgcga cgcccctgat tccacaacca gtgaccgatg
    10921 cgataggcct gcaaggcatg aaaacccttc aggtataaca acggggttga gtatttatcg
    10981 actgccgggt cgcgggtacg caccgcctga atatcacagg ccgcagaggc gatcatttcc
    11041 gggtcagcgg cgtaggcttc ttccaccact tcacggatag caatagcagg cataattggc
    11101 gatgacagct tgttcgccag catgtagctc agtgcactac caaggttttc gtgcttgagt
    11161 agcgtcgcgt ggtaaaaact ggccagcatt ggctcacagt ccgccagcgt tctggcttcg
    11221 gctttaatat tgttccagac aatttccagt tcttcacacg acattgctta ctccacacga
    11281 tgagataatg accggcccgt tctgcgcggg ccgggtcata gcggtaacaa aggttccctg
    11341 gggttagtgg ctgctgcgct cgtccttgcg tgcacgacct aataacgtca atgctgcctc
    11401 gcgcgcgttt tttccgcaat ataatacttg ataaatttcc tcggttattg gcatttctac
    11461 gccgaagcga tgcgccagtt cgcggacttc tttcgtattg cggtagcctt ccaccacctg
    11521 accaatcttc tcctgcgcgc tttgtacatc catgccctga ccgagcatca tgccaaaacg
    11581 gcggttacgc gactggttgt cggtacaggt aagcaccaga tcgcccagcc ccgccatgcc
    11641 cataaaggtg gcaggatcgg cacccagcgc cgcgccaaga cgcgacattt cagccagccc
    11701 acgggtgatc agtgccgtac gcgcattcgc accaaaaccg ataccgtcgg acatccccgc
    11761 gccaatggca atgacgtttt tcaccgcacc gccaagctgc acaccgatga aatccgggtt
    11821 gctgtaaacg cggaaacttt tgccacagtg cagcaattgt tggagatcat cggcaaaggt
    11881 ctggtcggta gaggccaacg aaatagccgt cggtaaacct gccgccagtt ctttcgcaaa
    11941 cgttgggcca gagataaccg ccagcggaat ttgatcgcct aaggcctcac gcgccacgtc
    12001 ctgtaacaga cgtccggttt ccgcttccag ccctttggtc gcccacacca gacgcgcatc
    12061 aggacgcatc agcggtttaa tctggcgcag cacttcacca aagacatggc tgggtacgac
    12121 gacgagaata ttacggctgg ctgccagcgc agtggcgaga tcgctttcaa gatggagcgt
    12181 atcgggaaaa ggcacatcgg ggagaaacgc ggcgttacag cggtcgcgtt caagcgttgc
    12241 gatatgttca gggtcatggc cccagaggac aacctcgtgg ccatttcttg ccagggtaat
    12301 ggcaagagcg gtgccgtacg agccggcacc gatcacagtc attgaagcat tacgttggtt
    12361 catcaggcat cctgatgttc ttcagtacct tcgccagcct gctgctgcaa atagttcatg
    12421 aacagcgcat cgaagttaat cggcgcaagg ttcagttgcg ggaatgtacc gcgggatacc
    12481 acgctggtga tgcactcacg agcatacggg aacagaatgt tcgggcagta tgcgcccagg
    12541 caatgtgcca tctgggtgcc ttcgataccc gcgatggaga aaataccgcc ctgctgaact
    12601 tcacacagga acgcggtttc ttcgcccagg gaggccgtta cggtaacacg cagtaccact
    12661 tcgtatacgt catctgccag ttgggaagat gccgtatcca gatcaagttt aacttctggt
    12721 tgccaatctt tctggaaaac gtgcggcgcg ttcggcgctt cgaaagagat atccttggta
    12781 taaatacgtt ggatctggaa agtcatttca gtgttgtttt gttctgacat gtgtagaaaa
    12841 cccttaagtg ttgtccttaa atactgcgca atgtaatgcc aacgttcaca cgttatttca
    12901 gcaggggatc cagtccacca cgtgcatcca atgcatacaa gtcatcacag ccgccaatgt
    12961 gctgtgcgtc aataaaaatc tggggaaccg tggtgcgacc gctgcgtttg atcatctctt
    13021 cacgcttggc ggcattgcca tcgatcggca actcctgaaa actcacgccc ttgctgctca
    13081 gcagtgcttt tgcacgatgg caatacgggc aggtttcttt ggtatagatt tcaacattgg
    13141 ccataattta gctcctgttt ttttaccctg aaatttcatg ttgcagggcg gctgcaaaat
    13201 ggagtgtccc cagaagctta taacaataag tcgctggggg cctaattgca gctaacggcc
    13261 tgcatcatga aagacgacag gtaaattatt tgccgcgcac cagaggcaag ttttcgccag
    13321 cccagccagc aacgccctct ttcagcacga atacttgcgc aaaaccagct ttcgtcagcg
    13381 cgtttgcagg ctcctggcac tgcatgccag aaccgtctac cacgataacc ggtttgtctt
    13441 tgtgcttctc aagctcacca acattgttgg ctttgatttc gctcggcaac agattgatag
    13501 aacctgcgat atggccttta cggaagtcat cacgctgacg taaatccaca accacagcgt
    13561 cttctttgtt gatcagacgc gtagcttcac cacgagtaat caccttcact ttcgaggtca
    13621 ggctcttaaa cgtagtcaca agaaccgcca ccagtaacgc gatccaggcg atactcagta
    13681 tgggatgacg gccaacaaat tgcataattt cttgcatggg gggtaacaac tcccgacgta
    13741 gtgattaaaa aaaccaggaa aggagtatac ctgcgcggtg cggcaaatac agccagcgcg
    13801 ttaactggaa tgcaattttg cggggcacga cgaaaaaaaa gcgcaaatct ggcatcggct
    13861 tgccgcgagc ggtcgtatat tttgatcttc agaggctatt ttatcgattc agctgtagta
    13921 aaattacgca aattttgact cttgagtatg aggttgtcgc aatgtcggtt tctaaaaaac
    13981 ctatggtact ggtgattctg gatggctatg gctatcgtga agaacagcag gataacgcca
    14041 tttttagtgc taaaaccccg gtaatggatg cactgtgggc caatcgtccg cataccctga
    14101 tcgacgcttc cggtctggaa gtcggtctgc ctgaccgtca gatgggtaac tccgaagtag
    14161 gccacgttaa cctgggtgcc ggccgcatcg tgtatcagga cctgactcgt ctggacgttg
    14221 aaatcaaaga tcgcgctttc tttgctaatc cagtgctgac tggcgcggta gataaagcga
    14281 aaaacgcagg taaagcagta cacattatgg gtctgctctc cgcaggcggc gtacacagcc
    14341 acgaagatca catcatggcg atggtagaac tggcagctga acgcggcgca gaaaaaatct
    14401 acctgcacgc attccttgac ggtcgcgaca ccccgccgcg cagtgctgaa tcctcgctga
    14461 aaaaatttga agagaaattt gctgcgctgg gtaaaggccg cgtagcgtcc atcattggtc
    14521 gttactacgc gatggaccgc gataaccgtt gggatcgcgt tgaaaaagcc tatgacctgc
    14581 tgactctggc gcagggtgag tttcaggccg atactgccgt tgctggtttg caggcagctt
    14641 atgctcgcga cgaaaacgac gagttcgtga aagcgaccgt tattcgtgca gaaggtcagc
    14701 cagatgcggc aatggaagac ggcgatgcgc tgattttcat gaacttccgt gctgaccgcg
    14761 cgcgtgaaat cactcgtgca ttcgtgaacg ctgatttcga tggcttcgcg cgtaagaaag
    14821 tggttaacgt cgatttcgtg atgctgaccg aatacgccgc tgacatcaaa actgcggttg
    14881 cttacccacc cgcttccctg gttaacacct tcggcgagtg gatggcgaaa aacgacaaaa
    14941 ctcagttgcg tatttccgaa accgaaaaat atgcccacgt tactttcttc ttcaacggtg
    15001 gcgtagaaga gtcgttcaaa ggcgaagatc gcattctgat caactcaccg aaagtggcta
    15061 cctacgatct gcaaccggaa atgagctccg cagagctgac cgaaaaactg gttgcggcca
    15121 tcaagagcgg caaatacgac accattatct gtaactatcc gaacggcgac atggtaggtc
    15181 acaccggggt gatggaagcg gcggttaaag cggttgaagc actggatcac tgcgtggaag
    15241 aagtcgcgaa agcggttgag tccgtgggtg gacaactgct gatcaccgct gaccacggta
    15301 acgctgaaca gatgcgcgat ccggcaacgg gtcaggcaca cacggcacac accaacctgc
    15361 cagttccgct gatttacgtt ggtgataaga acgtgaaagc ggttgcaggc ggcaaacttt
    15421 ctgacatcgc gccgaccatg ttgtcgctga tgggtatgga aatcccgcaa gagatgactg
    15481 gtaagccgct gttcatcgtg gaataatccc tccccatgag gggaaaggcg attaatacca
    15541 tgacacgggc cgtgaaaccg cgcaggtttg caatcaggcc catcatctac gccagcgttc
    15601 ttagcgctgg cgtattgttg tgcgcctttt ccgcccacgc ggatgagcgt gaccaactca
    15661 aatctattca ggccgatatc gccacaaaag agcgcgcggt acgccaaaag caacaacaac
    15721 gcgcaagcct gctcgcacaa ttgaaaaagc aggaagaagc gatctctgaa gccacccgta
    15781 agctgcgcga aacgcaaaac acgctcaatc aactcaataa acagattgat gagatgaacg
    15841 cgtcgattgc caaactggag cagcaaaaag ccgcccagga gcgcagcctc gccgcacaac
    15901 tggatgccgc attccgtcag ggcgagcata ccggtattca gctaattctc agcggtgaag
    15961 aaagccagcg tggacagcgt ttacaggctt atttcggcta tctcaaccag gcgcgacaag
    16021 aaaccattgc ccagttgaag caaacgcgtg aagaagtcgc catgcagcgt gctgaactgg
    16081 aagagaaaca aagcgagcaa caaacgctgt tatatgaaca gcgcgcccaa caggcgaaac
    16141 tgactcaggc tctgaacgag cgtaaaaaga cgctggcggg gctggaatct tccatccagc
    16201 aaggtcagca gcagctaagt gaactgcgtg ccaacgaatc ccgtctgcgt aacagtattg
    16261 cccgtgcgga agccgcggcg aaagcgcgtg ctgaacgaga agcgcgtgag gctcaggcag
    16321 ttcgcgacag gcagaaagaa gcgacgcgca aaggcaccac ctacaaaccg accgaaagcg
    16381 aaaaatcgct gatgtcccga actggtggcc tgggtgcgcc gcgtggtcag gcattctggc
    16441 cagttcgtgg ccctacgctg catcgctatg gcgagcagct acagggtgaa ttacgctgga
    16501 aaggaatggt tatcggtgcc tctgaaggta ctgaagttaa agcaattgcc gacggtcggg
    16561 tgattctggc tgactggctg caaggctacg gtctggtggt ggtggttgag cacggtaaag
    16621 gcgatatgag tctttacggc tataatcaaa gcgcgctggt gagcgttggt tcgcaggttc
    16681 gtgcgggcca gccaattgca ctggtgggca gcagtggcgg tcagggcaga ccttcactct
    16741 atttcgaaat tcgccgccag ggtcaggccg tcaatccaca gccgtggttg ggaagataag
    16801 ttttgtttcc atttcgtcgt aacgttcttg catttgccgc tctgttggcg ctctcctccc
    16861 ctgtaattgc tggcaaactt gccatcgtca ttgatgattt tgggtatcgc ccgaacaacg
    16921 aaaaccaggt gctggcgatg ccttccgcta tctccgtcgc tgtattaccc gattcaccgc
    16981 acgccagaga gatggcgacc aaagcgcata acagcggtca cgaagtgttg attcatctcc
    17041 cgatggcacc gttgagtaaa cagccgctgg agaaaaatac gctgcgcccg gagatgagca
    17101 gcgacgaaat tgagcgcatt attcgtagtg cggtcaataa cgtgccctat gccgtgggga
    17161 tcaataacca catgggcagc aagatgacct ctaacctgtt tggtatgcag aaagtgatgc
    17221 aggcgctgga gcgttataat ctttacttcc tcgacagcgt aaccattggt aatacccagg
    17281 cgatgcgcgc cgcgcaaggc actggcgtga aggtgatcaa acggaaggtg ttcctcgacg
    17341 attcacagaa cgaagcggac atccgtgtgc aattcaatcg cgcaattgac ctagcgcgtc
    17401 gcaacggatc aaccattgcc attgggcatc ctcacccttc aacggtacgc gtgttgcaac
    17461 agatggttta taacctgccg ccagacatta cgctggtgaa agccagcagc ttgctgaatg
    17521 aaccgcaggt tgatacgtct acaccgccga aaaacactgt gcccgacaca ccgcgtaatc
    17581 cgttccgtgg cgtgaagctg tgcaaaccga agaaaccgat agaacctgtt tatgctaatc
    17641 gcttctttga agtattaagc gaaagcatca gccagagcac gctgatcgtt tacttccagc
    17701 atcagtggca aggctggggc aaacagcctg aagcggcgaa gcttaacgct agcgtaaatt
    17761 aaaggcgcta cgcgtaatgc gatggtgcga ccgcgttttg tcgcgccatt gccataagcg
    17821 gaaagaccac agtaacgcct gataacccac ctttacgctg cgcacattgg taatcaggcg
    17881 cttatacata c
//
LOCUS       DAZPDU010000084        17771 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZPDU010000084 DAZPDU010000000
VERSION     DAZPDU010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17771
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.085"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(96..1379)
                     /gene="gltA"
                     /locus_tag="ACHURS_002525"
     CDS             complement(96..1379)
                     /gene="gltA"
                     /locus_tag="ACHURS_002525"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFR1238226.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            complement(1585..1692)
                     /locus_tag="ACHURS_002526"
     CDS             complement(1585..1692)
                     /locus_tag="ACHURS_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1238227.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            2073..2477
                     /gene="sdhC"
                     /locus_tag="ACHURS_002527"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             2073..2477
                     /gene="sdhC"
                     /locus_tag="ACHURS_002527"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFR1238228.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2471..2818
                     /gene="sdhD"
                     /locus_tag="ACHURS_002528"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2471..2818
                     /gene="sdhD"
                     /locus_tag="ACHURS_002528"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFR1238229.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            2818..4584
                     /gene="sdhA"
                     /locus_tag="ACHURS_002529"
     CDS             2818..4584
                     /gene="sdhA"
                     /locus_tag="ACHURS_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFR1238230.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4600..5316
                     /gene="sdhB"
                     /locus_tag="ACHURS_002530"
     CDS             4600..5316
                     /gene="sdhB"
                     /locus_tag="ACHURS_002530"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFR1238231.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            5617..8418
                     /gene="sucA"
                     /locus_tag="ACHURS_002531"
     CDS             5617..8418
                     /gene="sucA"
                     /locus_tag="ACHURS_002531"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFR1238232.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKENMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRTASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            8433..9650
                     /gene="odhB"
                     /locus_tag="ACHURS_002532"
     CDS             8433..9650
                     /gene="odhB"
                     /locus_tag="ACHURS_002532"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFR1238233.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            9744..10910
                     /gene="sucC"
                     /locus_tag="ACHURS_002533"
     CDS             9744..10910
                     /gene="sucC"
                     /locus_tag="ACHURS_002533"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFR1238234.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     CELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            10910..11779
                     /gene="sucD"
                     /locus_tag="ACHURS_002534"
     CDS             10910..11779
                     /gene="sucD"
                     /locus_tag="ACHURS_002534"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFR1238235.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(11883..12605)
                     /gene="mngR"
                     /locus_tag="ACHURS_002535"
     CDS             complement(11883..12605)
                     /gene="mngR"
                     /locus_tag="ACHURS_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFR1238236.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEILIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            12774..14690
                     /gene="mngA"
                     /locus_tag="ACHURS_002536"
     CDS             12774..14690
                     /gene="mngA"
                     /locus_tag="ACHURS_002536"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFR1238237.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            14708..17341
                     /gene="mngB"
                     /locus_tag="ACHURS_002537"
     CDS             14708..17341
                     /gene="mngB"
                     /locus_tag="ACHURS_002537"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFR1238238.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLQDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGSLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITAANAKPQCDIIHEAWQSRAVIR
                     YDMAVPLNLSERSARQSTGRVGGVLVVTLSHNSRRIDVDINLDNQADDHRLRVLVPTP
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYVALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
BASE COUNT         4307 a         4404 c         4813 g         4247 t
ORIGIN      
        1 gattttattt ttacaatttt ttgtgtttgg gcatataaaa attaacccgc catttgaacg
       61 gcgggttaaa atatttacaa cttagcaatc aaccattaac gcttgatatc gcttttaaag
      121 tcgcgttttt catatcctgt atacagctga cgcggacggg caatcttcat accgtcactg
      181 tgcatttcgc tccagtgggc gatccagcca acggtacgtg ccattgcgaa aatgacggtg
      241 aacatggaag acggaatacc catcgctttc aggatgatac cagagtagaa atcgacgttc
      301 gggtacagtt tcttctcgat aaagtacggg tcgttcagcg cgatgttttc cagctccata
      361 gccacttcca gcaggtcatc tttggtgccc agctctttca gaacttcatg gcaggtttca
      421 cgcattacgg tggcgcgcgg gtcgtaattt ttgtacacgc ggtgaccgaa gcccatcagg
      481 cggaaagaat catttttatc tttcgcacga cgaacaaatt ccggaatgtg tttaacggag
      541 ctaatttctt ccagcatttt cagcgccgct tcgttagcac caccgtgcgc aggtccccac
      601 agtgaagcaa tacctgctgc gatacaggca aacgggttcg cacccgaaga gccagcggta
      661 cgcacggtgg aggtagaggc gttctgttca tggtcagcgt gcaggatcag aatacggtcc
      721 atagcacgtt ccagaatcgg attaacttca tacggttcgc acggcgtgga gaacatcata
      781 ttcaggaagt taccggcata ggagagatcg ttgcgcggat aaacaaatgg ctgaccaatg
      841 gaatacttgt aacacatcgc ggccatggtc ggcattttcg acagcaggcg gaacgcggca
      901 atttcacggt gacgaggatt gttaacatcc agcgagtcgt gatagaacgc cgccagcgcg
      961 ccggtaatac cacacatgac tgccattgga tgcgagtcgc gacggaaagc atggaacaga
     1021 cgggtaatct gctcgtggat catggtatga cgggtcaccg tagttttaaa ttcgtcatac
     1081 tgttcctgag tcggtttttc accattcagc aggatgtaac aaacttccag gtagttagaa
     1141 tcggtcgcca gctgatcgat cgggaaaccg cggtgcagca aaataccttc atcaccatca
     1201 ataaaagtaa ttttagattc gcaggatgcg gttgaagtga agcctgggtc aaaggtgaac
     1261 acaccttttg aaccgagagt acggatatca ataacatctt gacccagcgt gcctttcagc
     1321 acatccagtt caacagctgt gtccccgttg agggtgagtt ttgcttttgt atcagccatt
     1381 taaggtctcc ttagcgcctt attgcgtaag actgccggaa cttaaatttg ccttcgcaca
     1441 tcaacctggc tttacccgtt tgttatttgg ctcgccgctc tgtgaaagag gggaaaacct
     1501 gggtacagag ctctgggcgc ttgcaggtaa aggatccatt gatgacgaat aaatggcgaa
     1561 tcaagtactt agcaatccga attattaaac ttgtctacca ctaataactg tcccgaatga
     1621 attggtcaat acttccacac tgttacataa gttaatctta ggtgaaatac cgacttcata
     1681 acttttacgc attatatgct tttcctggca atgtttgtaa caactttgtt gaatgattgt
     1741 caaattagat gattaaaaat taaataaatg ttgttatcgt gacctggatc actgttcagg
     1801 ataaaacccg acaaactata tgtaggttaa ttgtaatgat tttgtgaaca gcctatactg
     1861 ccgccaggtc tccggaacac cctgcaatcc cgagccaccc agcgttgtaa cgtgtcgttt
     1921 tcgcatctgg aagcagtgtt ttgcatgacg cgcagttata gaaaggacgc tgtctgaccc
     1981 gcaagcagac cggaggaagg aaatcccgac gtctccaggt aacagaaagt taacctctgt
     2041 gcccgtagtc cccagggaat aataagaaca gcatgtgggc gttattcatg ataagaaatg
     2101 tgaaaaaaca aagacctgtt aatctggacc tacagaccat ccggttcccc atcacggcga
     2161 tagcgtccat tctccatcgc gtttccggtg tgatcacctt tgttgcagtg ggcatcctgc
     2221 tgtggcttct gggtaccagc ctctcttccc ctgaaggttt cgagcaagct tccgcgatta
     2281 tgggcagctt cttcgtcaaa tttatcatgt ggggcatcct taccgctctg gcgtatcacg
     2341 tcgtcgtagg tattcgccac atgatgatgg attttggcta tctggaagaa acattcgaag
     2401 cgggtaaacg ctccgccaaa atctcctttg ttattactgt cgtgctttca cttctcgcag
     2461 gagtcctcgt atggtaagca acgcctccgc attaggacgc aatggcgtac atgatttcat
     2521 cctcgttcgc gctaccgcta tcgtcctgac gctctacatc atttatatgg tcggtttttt
     2581 cgccaccagt ggcgaactga catatgaagt ctggatcggt ttcttcgcct ctgcgttcac
     2641 caaagtgttc accctgctgg cgctgttttc tatcttgatt catgcctgga tcggcatgtg
     2701 gcaggtgttg accgactacg ttaaaccgct ggcattgcgc ctgatgctgc aactggtgat
     2761 tgtcgttgca ctggtggttt acgtgattta tggattcgtt gtggtgtggg gtgtgtgatg
     2821 aaattgccag tcagagaatt tgatgcagtt gtgattggtg ccggtggcgc aggtatgcgc
     2881 gcggcgctgc aaatttccca gagcggccag acctgtgcgc tgctctctaa agtcttcccg
     2941 acccgttccc ataccgtttc tgcgcaaggc ggcattaccg ttgcgctggg taatacccat
     3001 gaagataact gggaatggca tatgtacgac accgtaaaag ggtcggacta tatcggtgac
     3061 caggacgcga ttgaatatat gtgtaaaacc gggccggaag cgattctgga actcgaacac
     3121 atgggcctgc cgttctcgcg tcttgatgat ggtcgtatct atcaacgtcc gtttggcggt
     3181 cagtcgaaaa acttcggcgg cgagcaggcg gcacgcactg cggcggcggc tgaccgtacc
     3241 ggtcacgcac tgttgcacac gctttatcag cagaacctga aaaaccacac caccattttc
     3301 tccgagtggt atgcgctgga tctggtgaaa aaccaggatg gcgcggtggt gggttgtacc
     3361 gcactgtgca tcgaaaccgg tgaagtggtt tatttcaaag cccgcgctac cgtgctggcg
     3421 actggcgggg cagggcgtat ttatcagtcc accactaacg cccacattaa cactggcgac
     3481 ggtgtcggca tggctatccg cgcaggcgta ccggtgcagg atatggaaat gtggcaattc
     3541 cacccgaccg gcattgccgg tgcgggcgta ctggtcaccg aaggttgccg tggtgaaggc
     3601 ggttatctgc tgaacaaaca tggcgaacgc tttatggagc gttatgcgcc gaacgccaaa
     3661 gacctggcgg gccgtgatgt ggtggcgcgt tccatcatga ttgaaatccg tgaaggtcgc
     3721 ggctgtgatg gtccgtgggg gccacacgcg aaactgaaac tcgatcacct gggtaaagaa
     3781 gttctcgaat cccgtctgcc gggtatcctg gagctttccc gtacctttgc tcacgtcgat
     3841 ccggtgaaag agccgattcc ggttatccca acctgtcact acatgatggg cggtattccg
     3901 accaaagtga ccggtcaggc gctgacggtg aacgagaaag gcgaagatgt ggttgttccg
     3961 gggctgtttg ccgttggtga aatcgcttgt gtatcggtac acggcgctaa ccgtctgggc
     4021 ggcaactcgc tgctggacct ggtggtcttt ggtcgtgcgg caggtctgca tctgcaagag
     4081 tctatcgccg aacagggcgc attgcgcgat gccagcgagt ctgatgttga agcatctctg
     4141 gatcgtctga accgctggaa caataatcgt aacggtgaag atccggtggc gattcgtaaa
     4201 gcgctgcaag aatgtatgca gcataacttc tcggtcttcc gtgaaggtga tgcgatggcg
     4261 aaagggcttg agcagttgaa agtgatccgc gagcgtctga aaaatgcccg tctggatgac
     4321 acttccagcg agttcaacac ccagcgcgtt gagtgcctgg aactggataa cctgatggaa
     4381 acggcgtatg caacggctgt ttctgccaac ttccgtaccg aaagccgtgg cgcacatagc
     4441 cgcttcgact tcccggatcg tgatgatgaa aactggctgt gccactccct gtatctgcca
     4501 gagtcggaat ccatgacgcg ccgaagcgtc aacatggaac cgaaactgcg cccggcattc
     4561 ccgccgaaga ttcgtactta ctaatgcgga gacaggaaaa tgagactcga gttttcaatt
     4621 tatcgctata acccggatgt tgatgatgct ccgcgtatgc aggattacac cctggaagcg
     4681 gaagaaggtc gcgacatgat gctgctggat gcgcttatcc agctgaaaga gaaagatccc
     4741 agcctgtcgt tccgccgctc ctgccgtgaa ggtgtgtgcg gttccgacgg tctgaacatg
     4801 aacggcaaga atggtctggc ctgtattacc ccgatttcgg cactcaacca gccgggcaag
     4861 aagattgtga ttcgcccgct gccaggttta ccggtgatcc gcgatttggt ggtagacatg
     4921 ggacaattct atgcgcaata tgagaaaatt aagccttacc tgttgaataa tggacaaaat
     4981 ccgccagctc gcgagcattt acagatgcca gagcagcgcg aaaaactcga cgggctgtat
     5041 gaatgtattc tctgcgcatg ttgttcaacc tcttgtccgt ctttctggtg gaatcccgat
     5101 aagtttatcg gcccggcagg cttgttagcg gcatatcgtt tcctgatcga tagccgtgat
     5161 accgagactg acagccgcct cgacggtttg agcgatgcat tcagcgtatt ccgctgtcac
     5221 agcatcatga actgcgtcag tgtatgtccg aaggggctga acccgacgcg cgccatcggc
     5281 catattaagt cgatgttgtt gcaacgtaat gcgtaaaccg taggcctgat aagacgcgca
     5341 agcgtcgcat caggcaacca gtgccggatg cggcgtgaac gccttatccg gcctacaagt
     5401 cattacccgt aggcctgata agcgcagcgc atcaggcgta acaaagaaat gcaggaaatc
     5461 tttaaaaact gcccctgaca ctaagacagt ttttaaaggt tccttcgcga gccactacat
     5521 agacaagagc tcgcaagtga accccggcac gcacatcact gtgcgtggta gtatccacgg
     5581 cgaagtaagc ataaaaaaga tgcttaaggg atcacgatgc agaacagcgc tttgaaagcc
     5641 tggttggact cttcttacct ctctggcgca aaccagagct ggatagaaca gctctatgaa
     5701 gacttcttaa ccgatcctga ctcggttgac gctaactggc gttcgacgtt ccagcagtta
     5761 cctggtacgg gagtcaaacc ggatcaattc cactctcaaa cgcgtgaata tttccgccgc
     5821 ctggcgaaag acgcttcacg ttactcttca acgatctccg accctgacac caatgtgaag
     5881 caggttaaag tcctgcagct cattaacgca taccgcttcc gtggtcacca gcatgcgaat
     5941 ctcgatccgc tgggactgtg gcagcaagat aaagtggccg atctggatcc gtctttccac
     6001 gatctgaccg aagcagactt ccaggagacc ttcaacgtcg gttcatttgc cagcggcaaa
     6061 gaaaacatga aactcggcga gctgctggaa gccctcaagc aaacctactg tggcccgatt
     6121 ggtgccgagt atatgcacat taccagcacc gaagaaaaac gctggatcca acagcgtatc
     6181 gagtctggtc gcgcgacttt caatagcgaa gagaaaaaac gcttcttaag cgaactgacc
     6241 gccgctgaag gccttgaacg ttacctcggc gcaaaattcc ctggcgcaaa acgcttctcg
     6301 ctggaaggcg gtgacgcgtt aatcccgatg cttaaagaga tgatccgcca cgctggcaac
     6361 agcggcaccc gcgaagtggt tcttgggatg gcgcaccgtg gtcgtctgaa cgtgctggtg
     6421 aacgtgctgg gtaaaaaacc gcaagacttg ttcgacgagt ttgccggtaa acataaagaa
     6481 cacctcggca cgggtgacgt gaaataccac atgggcttct cgtctgactt ccagaccgat
     6541 ggcggcctgg tgcacctggc gctggcgttt aacccgtctc accttgagat tgtaagcccg
     6601 gtagttatcg gttctgttcg tgcccgtctg gacagacttg atgagccgag cagcaacaaa
     6661 gtgctgccaa tcaccatcca cggtgacgcc gcagtgaccg ggcagggcgt ggttcaggaa
     6721 actctgaaca tgtcgaaagc gcgtggttat gaagttggcg gtacggtacg tatcgttatc
     6781 aacaaccagg ttggtttcac cacctctaat ccactggatg ctcgttctac accgtactgt
     6841 actgatatcg gtaagatggt tcaggcaccg attttccacg ttaacgcgga tgatccggaa
     6901 gccgttgcct ttgtgacccg tctggcgctc gatttccgta acacctttaa acgtgatgtc
     6961 ttcatcgacc tggtgtgcta ccgccgtcac ggccacaacg aagccgacga gccgagcgca
     7021 acccagccgc tgatgtatca gaaaatcaaa aaacatccga caccgcgcaa aatctacgct
     7081 gacaagctgg agcaggaaaa agtggcgacg ctggaagatg ccaccgagat ggttaacctg
     7141 taccgcgatg cgctggatgc tggcgattgc gtagtggcag agtggcgtcc gatgaacatg
     7201 cactctttca cctggtcgcc gtacctcaac cacgaatggg acgaagagta cccgaacaaa
     7261 gttgagatga agcgcctgca ggagctggcg aaacgcatca gcacggtgcc ggaagcagtt
     7321 gaaatgcagt ctcgcgttgc caagatttat ggcgatcgcc aggcgatggc tgccggtgag
     7381 aaactgttcg actggggcgg tgcggaaaac ctcgcttacg ccacgctggt tgacgaaggc
     7441 attccggttc gcctgtcggg tgaagactcc ggtcgcggta ccttcttcca ccgccacgcg
     7501 gtgatccaca accagtctaa cggttccact tacacgccgt tgcaacacat ccataacggg
     7561 cagggcgcgt tccgcgtctg ggactccgta ctgtctgaag aagcagtgct ggcgtttgaa
     7621 tacggttatg ccaccgcaga accacgcact ctgaccatct gggaagcgca gttcggtgac
     7681 ttcgccaacg gtgcgcaggt ggttatcgac cagttcatct cttctggcga acagaaatgg
     7741 ggccggatgt gtggtctggt gatgttgctg ccgcacggtt acgaagggca ggggccggag
     7801 cactcctccg cgcgtctgga acgttatctg caactttgtg ctgagcaaaa catgcaggtt
     7861 tgcgttccgt ctaccccggc acaggtttac cacatgctgc gtcgtcaggc gctgcgcggg
     7921 atgcgtcgtc cgctggtagt gatgtcgccg aaatccctgc tgcgtcatcc gctggcggtt
     7981 tccagcctcg aagaactggc gaacggcacc ttcctgccag ccatcggtga aatcgacgag
     8041 cttgatccga agggcgtgaa gcgcgtagtg atgtgttctg gtaaggttta ttacgacctg
     8101 ctggaacagc gtcgtaagaa caatcaacac gatgtcgcca ttgtgcgtat cgagcaactc
     8161 tacccgttcc cgcataaagc gatgcaggaa gtgttgcagc agtttgctca cgtcaaggat
     8221 tttgtctggt gccaggaaga gccgctcaac cagggcgcat ggtactgcag ccagcatcat
     8281 ttccgtgaag tgattccgtt tggggcttct ctgcgttatg caggccgcac ggcctccgcc
     8341 tctccggcgg tagggtatat gtccgttcac cagaaacagc aacaagatct ggttaatgac
     8401 gcgctgaacg tcgaataaat aaaggataca caatgagtag cgtagatatt ctggtccctg
     8461 acctgcctga atccgtagcc gatgccaccg tcgcaacctg gcataaaaaa cccggcgacg
     8521 cagtcgtacg tgatgaagtg ctggtagaaa tcgaaactga caaagtggta ctggaagtac
     8581 cggcatcagc agacggcatt ctggatgcgg ttctggaaga tgaaggtaca acggtaacgt
     8641 ctcgtcagat ccttggtcgc ctgcgtgaag gcaacagcgc cggtaaagaa accagcgcca
     8701 aatctgaaga gaaagcgtcc actccggcgc aacgccagca ggcgtctctg gaagagcaaa
     8761 acaacgatgc gttaagcccg gcgatccgtc gcctgctggc tgaacacaat ctcgacgcca
     8821 gcgccattaa aggcaccggt gtgggtggtc gtctgactcg tgaagatgtg gaaaaacatc
     8881 tggcgaaagc cccggcgaaa gagtctgctc cggcagcggc tgctccggcg gcgcaaccgg
     8941 ctctggctgc acgtagtgaa aaacgtgtcc cgatgactcg cctgcgtaag cgtgtggcag
     9001 agcgtctgct ggaagcgaaa aactccaccg ccatgctgac cacgttcaac gaagtcaaca
     9061 tgaagccgat tatggatctg cgtaagcagt acggtgaagc gtttgaaaaa cgccacggca
     9121 tccgtctggg ctttatgtcc ttctacgtga aagcggtggt tgaagccctg aaacgttacc
     9181 cggaagtgaa tgcttctatc gacggtgatg acgtggttta ccacaactat ttcgacgtca
     9241 gcatggcggt ttccacgccg cgcggcctgg tgacgccggt actgcgtgat gtcgataccc
     9301 tcggcatggc agacatcgag aagaaaatca aagagctggc agttaaaggc cgtgacggca
     9361 agctgacggt tgaagatctg accggtggta acttcaccat caccaacggt ggtgtgttcg
     9421 gttccctgat gtctacgccg atcatcaacc cgccgcagag cgcaattctg ggtatgcacg
     9481 ctatcaaaga tcgtccgatg gcggtgaatg gtcaggttga gatcctgccg atgatgtacc
     9541 tggcgctgtc ctacgatcac cgtctgatcg atggtcgcga atccgtgggc ttcctggtaa
     9601 cgatcaaaga gttgctggaa gatccgacgc gtctgctgct ggacgtgtag tagtttaagc
     9661 ttcacctgca ctgtagaccg acgtggatag ggtgtcggtc tacggtttaa aagataacga
     9721 ttactgaagg atggacagaa cacatgaact tacatgaata tcaggcaaaa caactttttg
     9781 cccgctatgg cttaccagca ccggtgggtt atgcctgtac tactccgcgc gaagcagaag
     9841 aagccgcttc aaaaatcggt gccggcccgt gggtagtgaa atgtcaggtt cacgctggtg
     9901 gccgcggtaa agcgggcggt gtgaaagttg taaacagcaa agaagacatc cgtgcttttg
     9961 cagaaaactg gctcggcaag cgtctggtaa cgtatcaaac agatgccaat ggccaaccgg
    10021 ttaaccagat tctggttgaa gcagcgaccg atatcgctaa agagctgtat ctcggtgccg
    10081 ttgtagaccg tagttcccgt cgtgtggtct ttatggcctc caccgaaggc ggcgtggaaa
    10141 tcgaaaaagt ggcggaagaa actccgcacc tgatccacaa agttgcgctt gatccgctga
    10201 ctggcccgat gccgtatcag ggatgcgagc tggcgttcaa actgggtctg gaaggtaaac
    10261 tggttcagca gttcaccaaa atcttcatgg gcctggcgac cattttcctg gagcgcgacc
    10321 tggcgttgat cgaaatcaac ccgctggtca tcaccaaaca gggcgatctg atttgcctcg
    10381 acggcaaact gggcgctgac ggcaacgcac tgttccgcca gcctgatctg cgcgaaatgc
    10441 gtgaccagtc gcaggaagat ccgcgtgaag cacaggctgc acagtgggaa ctgaactacg
    10501 ttgcgctgga cggtaacatc ggttgtatgg ttaacggcgc aggtctggcg atgggtacga
    10561 tggacatcgt taaactgcac ggcggcgaac cggctaactt ccttgacgtt ggcggcggcg
    10621 caaccaaaga acgtgtaacc gaagcgttca aaatcatcct ctctgacgac aaagtgaaag
    10681 ccgttctggt taacatcttc ggcggtatcg ttcgttgcga cctgatcgct gacggtatca
    10741 tcggcgcggt agcagaagtg ggtgttaacg taccggtcgt ggtacgtctg gaaggtaaca
    10801 acgccgaact cggcgcgaag aaactggctg acagcggcct gaatattatt gcagcaaaag
    10861 gtctgacgga tgcagctcag caggttgttg ccgcagtgga ggggaaataa tgtccatttt
    10921 aattgataaa aacaccaagg ttatctgcca gggctttact ggtagccagg ggactttcca
    10981 ctcagaacag gccattgcat acggcactaa aatggttggc ggcgtaaccc caggtaaagg
    11041 cggtactacc cacctcggcc tgccggtgtt caacaccgtg cgtgaagctg ttgctgctac
    11101 tggcgctacc gcttctgtta tctacgtacc agcaccgttc tgcaaagact ccattctgga
    11161 agctattgat gcaggcatca aactgattat caccatcact gaaggcatcc cgacgctgga
    11221 tatgctgact gtgaaagtga aactggacga agccggcgtt cgtatgatcg gcccgaactg
    11281 cccgggcgtt atcactccgg gtgaatgcaa aatcggtatc cagccgggtc acattcacaa
    11341 accgggtaaa gtgggtatcg tttcccgttc cggtacactg acctatgaag cggttaaaca
    11401 gaccacggat tacggtttcg gtcagtcgac ctgtgttggt atcggcggtg acccgatccc
    11461 aggctctaac tttatcgaca tcctcgaaat gttcgaaaaa gatccgcaga ccgaagcgat
    11521 cgtgatgatc ggtgagatcg gcggtagcgc tgaagaagaa gcggcagcgt acatcaaaga
    11581 gcacgttacc aagccagttg taggttacat cgctggtgtg actgcgccga aaggcaaacg
    11641 tatgggccac gcgggtgcga tcattgctgg tgggaaaggg actgcggatg agaaattcgc
    11701 tgctctggaa gccgcaggcg tgaaaaccgt tcgcagcctg gcggatatcg gtgaagcact
    11761 gaaaactgtt ctgaaataaa tatctgtaat aagaaatagc cctcgccgct tccctctaca
    11821 ggaatggcga agggctgtcg gtttcgacat ggttggccat cgtatgatgg ccttttttgt
    11881 gcttatcgcg atgattttcg ctgcgctatc agggtaaatt tatagtcatc ggtattaaaa
    11941 gcgttgcggc tatattcaaa cacccgacca tcaactaaat atccacgcga tactttttca
    12001 agaatcggct ttgtctggct gatattaagc agacggctca tctcttcggt tggcatcaga
    12061 ggaatgattt cctgttcgct acgatcgata accatcttct tcacttcttc aataaagtga
    12121 tatttcgaat tttccatgac ctgccaggtg agatccggga acaacgcaag cggcatccag
    12181 gtttcttcca gcgccattgg cttttgcttg cgatagcgca cgcgcttcac atgccacaca
    12241 cgatcctgcg cggtgatttg tagctgttgc tgaagaaaat cgtcagccgg aatcacttca
    12301 aatatcagga tttcactgtg tgtatcgacg tgacggtccg acagtttttc atcaaaactg
    12361 gttaactgaa aaatatcgta attgacccgc tcttctttga cgtaagtccc gctgccctga
    12421 atgctttcga ggatctgctg ctcgactagc tggcgcaaag cctgacgcac cgtaacccgg
    12481 ctgacgccaa actctgtttg tagcgctgat tcagtgggta acgcatcgcc aggtttaagc
    12541 tcgccacgcg caatttgttc acgaatgcga tcggcaatct gccggtataa gggcttgtgt
    12601 cccattttta gtatctcatt aatacgaatt tagccattat gcccgataaa ttcatcctgt
    12661 aaataataca aatacaatac aaataatttc aatcaagtga aattgatcac atgatggtat
    12721 tgttttatcg ggcacactgg cgcgactata aaaacgatca agtgaggatc atgatgaatc
    12781 tgacgactct gacccaccgc gatgcgttgt gtctgaatgc gcgctttacc agccgtgaag
    12841 aggccatcca cgcgttgact caacgtcttg ctgctctggg gaaaatttcc agtactgagc
    12901 aatttctgga agaagtgtat cgccgtgaaa gccttggccc gaccgcctta ggtgaagggt
    12961 tggctgtgcc gcatggcaaa actgctgcgg taaaagaagc ggcgtttgcg gtcgccacac
    13021 tcagcgagcc gcttcagtgg gaaggcgttg atggcccgga agcagttgat ttagtggtgc
    13081 tgctggcgat tccccccaat gaagcgggta ctacgcatat gcaactgctg acagcgctga
    13141 ccacgcgcct tgcggatgat gagattcggg cgcgtataca gtcggcgacg acgcctgatg
    13201 agttgctctc ggcgctggat gacaagggag gcacgcaacc ttctgcctct ttttccaacg
    13261 cgccaactat cgtctgcgta acggcctgtc cggcgggtat tgctcacacc tatatggctg
    13321 cggaatatct ggaaaaagcc ggacgcaaac tcggcgtaaa tgtttacgtt gaaaaacaag
    13381 gcgctaacgg cattgaaggg cgtttaacgg cggatcaact caatagtgca accgcctgta
    13441 tttttgcggc tgaagtcgcc atcaaggaga gtgagcgttt taatggcatt cccgcgcttt
    13501 cagtgcctgt tgccgagccg attcgccatg cagaagcgtt gatccaacaa gcgcttaccc
    13561 tcaagcgtag cgatgagacg cgtaccgtac agcaagatac gcaaccggtg aaaagtgtca
    13621 aaacggagct gaaacaggca ctgttgagcg gaatctcttt tgccgtaccg ttgattgtcg
    13681 cggggggcac ggtgctggcg gtcgcggtat tactgtcgca aatcttcggg ctacaagatc
    13741 tgtttaatga agaaaactcc tggctgtgga tgtaccgcaa gctgggcggc gggctgctcg
    13801 gaattttgat ggtaccggtg ctcgcggcct ataccgccta ttctctggca gataaaccgg
    13861 cgttagcgcc aggctttgcg gctggacttg ccgccaacat gatcggctcc gggtttctcg
    13921 gcgcggtcgt tggcggattg atagccggtt acttgatgcg ctgggtgaaa aatcacttgc
    13981 gtcttagcag taaattcaat ggattcctga ctttttatct ctacccggtg ctcggtacgt
    14041 tgggagcggg cagtctgatg ctgtttgtgg tgggagaacc tgtcgcatgg atcaataact
    14101 cgcttaccgc ctggctgaac ggtctgtcag gaagtaacgc gctgttgctg ggtgccattc
    14161 tcggttttat gtgttccttt gaccttggag ggccagtgaa caaagccgct tatgcattct
    14221 gcctgggcgc aatggcgaac ggcgtttacg gcccgtatgc cattttcgcc tccgtcaaaa
    14281 tggtttcggc atttaccgta accgcttcca cgatgctcgc accgcgcctg tttaaagagt
    14341 ttgaaattga gaccgggaaa tccacctggc tgttagggct ggcaggtatt accgaagggg
    14401 cgatcccgat ggcgattgaa gatccgctgc gggttattgg ttcgtttgtg ctgggctcta
    14461 tggtaacggg cgctattgtc ggtgcgatga atatcggcct ttcgacaccc ggtgccggca
    14521 ttttctcgct ctttttactt catgataatg gcgcgggcgg tgttatggcg gcaattggct
    14581 ggtttggtgc ggcattggtg ggggctgcaa tctcgactgc aattctcctg atctggcggc
    14641 gtcacgcggt taagcatggc aactatctga ctgatggcgt aatgccataa acaaaaagga
    14701 aacgacgatg aaagcggtat ctcgcgttca catcaccccg catatgcatt gggatcggga
    14761 gtggtatttc accaccgaag agtcacgcat tctgctggtc aataatatgg aagagatcct
    14821 gtgccgactg gaacaggaca acgaatacaa atattacgta ctcgacgggc aaacggcgat
    14881 cctcgaagat tatttcgcgg tgaaaccgga aaacaaagac cgcgtgaaga aacaggtaga
    14941 agccggtaag ttgattatcg gcccctggta tacccagacc gataccacga ttgtttctgc
    15001 ggaatccatc gtccgtaatc tgatgtacgg aatgcgtgac tgcctcgcgt ttggcgagcc
    15061 gatgaaaata ggttatttac cggattcctt tggcatgtcc gggcaactgc cgcatattta
    15121 caatggattt ggcattaccc gcactatgtt ctggcgcgga tgttcggagc gccacggtac
    15181 tgataaaacc gagtttttgt ggcaaagcag tgacggtagc gaagtgacgg cgcaggtgct
    15241 gccgctgggc tacgccatcg gtaagtactt acctgccgac gaaaacggat tacgtaaacg
    15301 cctcgacagt tattttgacg tgctggaaaa agcgtctgta accaaagaga ttttgctgcc
    15361 gaatggtcat gaccagatgc cattgcagca aaatatcttc gaagtgatgg ataagctacg
    15421 tgagatctac cctcaacgta agtttgtgat gagccgcttt gaagaggtat ttgagaagat
    15481 cgaagcgcag cgtgaaagtc tggccaccct gaaaggtgaa tttatcgatg gcaaatatat
    15541 gcgcgtacat cgcaccatcg gttccacgcg catggatatc aaaattgccc acgcacgtat
    15601 tgaaaataag attgttaatc tgctggaacc gctggcaacg ctggcctgga cgttgggttt
    15661 tgaataccac cacggcttgc tggagaaaat gtggaaagag atcttaaaaa atcatgccca
    15721 cgacagtatc ggttgttgtt gcagtgacaa agttcatcgc gaaattgtcg cccgcttcga
    15781 actggctgaa gacatggcgg ataatctgct gcgtttctac atgcgcaaaa ttgccgataa
    15841 catgccacag agtgatgccg acaaactcgt gctgtttaac ctgatgccct ggccgcggga
    15901 agaagttatt aacaccactg tgcggctgcg cgccagccag tttaatctac aagacgatcg
    15961 cggtcagcct gtaccgtatt ttattcgcca tgcccgtgag atcgatcctg gcctaatcga
    16021 tcggcaaatt gttcattatg gcaattatga tccctttatg gagtttgata ttcagatcaa
    16081 ccagattgtc ccttctatgg gctatcgcac gctttatatt gaagcgaatc agcctggcaa
    16141 cgtgattgcg gcaaaaagtg acgctgaagg gattctggaa aatgctttct ggcagattgc
    16201 gctcaatgag gatggttctc tgcaactggt agataaagac agcggtgtgc gctatgaccg
    16261 ggtattgcaa attgaagaaa gctctgatga tggtgatgaa tatgactatt cacccgcaaa
    16321 agaagagtgg gtaattaccg cagcgaacgc gaaaccgcaa tgcgatatta ttcatgaagc
    16381 ctggcagagc agggctgtta tccgctatga catggcagtg ccgctcaatt tgtcagaacg
    16441 cagcgcccgg caatccactg gcagagtagg gggggtgttg gttgtcactc ttagtcataa
    16501 cagcaggcgt attgatgtgg atatcaacct tgataaccag gctgacgatc atcgccttcg
    16561 tgtcctggtc cctacacctt ttaacactga cagtgttctg gcagatacgc agtttggttc
    16621 gctaacgcgc cccgtgaacg acagtgcaat gaacaactgg cagcaagaag gctggaaaga
    16681 agcgccggtt ccggtatgga atatgctcaa ctatgttgcc ttacaggaag ggcgtaacgg
    16741 catggctgtc tttagcgaag ggttacgtga atttgaagtc atcggtgaag agaagaaaac
    16801 ctttgccatt acgttgctgc gtggcgtggg cttactgggc aaagaagatc tgcttttaag
    16861 gcctgggcgg ccttcgggaa ttaaaatgcc agtcccggac tcacaactac gtggtctgct
    16921 ttcttgtcgc ctaagtttat tgagttatac cggtacgcca accgccgctg gtgtagctca
    16981 gcaggcgcga gcatggctga ctccagtaca gtgttacaac aaaatcccat gggatgcgat
    17041 gaagctcaac aaagccggat tcaacgtgcc ggaaagttat agtttgttga aaatgccccc
    17101 agtgggatgc ctgataagcg cacttaagaa agctgaagac cgacaagaag tgattttacg
    17161 gctgtttaat ccggctgaat cagcaacctg tgatgcgact gttgctttca gtcgcgaggt
    17221 gatttcttgc tcagaaacga tgatggatga acacattacc accgaggaaa atcaaggttc
    17281 aaatctatcg gggccttttt tacccggcca gtcacggacg ttcagttacc ggcttgcctg
    17341 aatagcaatc aaaccgaagc cacatatgcg cggccagatt gttgacaaag ggcgctttgt
    17401 tcatgccgga tacggcatga acgctttatt cggtctacaa aagcaggcaa attcaatata
    17461 ttgcagagat gatgtagaca ctggcaagcg tagcgcatca ggtaattttg cgtttatctt
    17521 cactctcaag ccacgtatat gtggctttat ttttaacaaa ataataaccc tgggtgagtt
    17581 aattataata taattacaag ttaattaaat gttaatatcg gcggggtgga tttatgcctt
    17641 tattagtaat cctgaaactc tgcgtcgtat tagccagtga ccaaaaaaag aattaaggtc
    17701 aaccgtgctg tttttgcttc gtctcttttt atctttaaat tgccaaccga aactaatttc
    17761 agccttataa c
//
LOCUS       DAZPDU010000085        17639 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZPDU010000085 DAZPDU010000000
VERSION     DAZPDU010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17639
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.086"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..195
                     /locus_tag="ACHURS_002538"
     CDS             <1..195
                     /locus_tag="ACHURS_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFR1238239.1"
                     /translation="TILGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHF
                     ENVKTRVQGTVVMFSASGKK"
     gene            complement(140..277)
                     /locus_tag="ACHURS_002539"
     CDS             complement(140..277)
                     /locus_tag="ACHURS_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleocapsid protein"
                     /protein_id="HFR1238240.1"
                     /translation="MAYEPCQGVIIGIMPQHVLSKNVLRLDAIFSLKRKTLLQYLEPW
                     F"
     gene            complement(250..348)
                     /locus_tag="ACHURS_002540"
                     /pseudo
     CDS             complement(250..348)
                     /locus_tag="ACHURS_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937495.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(337..576)
                     /locus_tag="ACHURS_002541"
                     /pseudo
     CDS             complement(337..576)
                     /locus_tag="ACHURS_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905063.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            675..1565
                     /locus_tag="ACHURS_002542"
                     /pseudo
     CDS             675..1565
                     /locus_tag="ACHURS_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533802.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            1568..2101
                     /locus_tag="ACHURS_002543"
     CDS             1568..2101
                     /locus_tag="ACHURS_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR1238241.1"
                     /translation="MQHLKNIVAGNPKTVEQYQLTKNFDVIWLWSEDGKNWHEELKNF
                     QEDTIKLAYTVEGIIIAIDKDVSAINPEGLSVVELPDITANRRADISGNWMFKDGAVI
                     KRTYTEEEQRQQAENEKQSLLQLVRDKTQLWDSQLQLGIISDENKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(2130..2657)
                     /locus_tag="ACHURS_002544"
     CDS             complement(2130..2657)
                     /locus_tag="ACHURS_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR1238242.1"
                     /translation="MMHLKNIKAGNPKTPEQYQLTKKAGVVWLFCEDGKNWYEEQKNF
                     QPDTIKIAYDENNIIVAVSKDVSTINPEGLSIVEVPDITANRHADNNGKWMFLDGKVV
                     KREYTEQELQQQAESQKAALLSEAESVIQPLERAVRLNMATDEERTRLEAWERYSVLV
                     SRVDTANPEWPQKPE"
     gene            complement(2659..5415)
                     /locus_tag="ACHURS_002545"
     CDS             complement(2659..5415)
                     /locus_tag="ACHURS_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR1238243.1"
                     /translation="MTVKISGVLKDGTGKPVQNCTIVLKARRTSSTMVVNTVASENPD
                     EAGRYSMDVEHGQYSVTLLVEGFPPSHAGTITVYEGSRPGTLNDFLGAMTEDDVRPEA
                     LRRFEQMVEEVSRNASAVAQNTAAAKKSASEAATHATDAAASARAASTSAGQAASSAQ
                     SASSSAGTASTKAREAAKSAAAESSKSAAATSASAAKTSETNAAASQKSAATSASTAT
                     TKASEAATSARGAAASKEAAKSSETNASSSASSAASSATAAGNSAKAAKTSETNAKSS
                     ETAAGQSASAAAGSKTAAALSASAASTSAGQASASATAAGKSAESAASSASTATTKAG
                     KATEQATAAARSASAAKTSETNAKTSADNAASSKAAAASSASSAASSASSASASKDEA
                     TRQASAAKGSATTATTKASEAAGSATAASQSKVAAESAATRAETAAKRAEDIASAVAL
                     EDASTTKKGIVQLSSATNSTSESLAATPKAVKAVMGETNKKAPLNSPALTGTPTTPTA
                     RQGTNNTQIASTAFVMAAIAALVDSSPDALNTLNELAAALGNDPNFATTMTNALAGKQ
                     PKDATLTALAGLTTAAGKFPYFTGNDVASLATLTKVGRDILAKSTVAAVIEYLGLQET
                     VNKAGNAVQRSGDKMTGELKIGTVNALRIFNDTFGLIFRRSEDFLHFIPTAEGQGENG
                     NIGPLRPFAINLRTGAISVSHGAKIKGGLAIGATDNALGENSIVLGDNDTGFRQDGDG
                     IISFYSNGSRIGHIDELGLHLYKDIESNGSNFRLKSNYRHHITFANEDGRIRMFLWKD
                     NGGDGVHINNGSDGGGDFIFKTDGGFALGSGAQVASSGDIYGSVWENNWLSTWLHNHV
                     VRDIRLGSIEYKNVWRDYGFGDASGYVLTAAINSNADDIVDTVARRPIQKLIGGIWYN
                     VGSV"
     gene            complement(5567..6166)
                     /locus_tag="ACHURS_002546"
     CDS             complement(5567..6166)
                     /locus_tag="ACHURS_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFR1238244.1"
                     /translation="MRKVCAAILSAAICLSVSGAPAWASEHQSTLSAGYLHARTNAPG
                     SDNLNGINVKYRYEFTDALGLITSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMVGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVTIDLAYEGSGSGDWRTDAFIVGIGYRI"
     gene            complement(6234..9685)
                     /locus_tag="ACHURS_002547"
                     /pseudo
     CDS             complement(6234..9685)
                     /locus_tag="ACHURS_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000515772.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            complement(10029..10709)
                     /locus_tag="ACHURS_002548"
     CDS             complement(10029..10709)
                     /locus_tag="ACHURS_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFR1238245.1"
                     /translation="MATTNAFSLASPPLARICLHGDLQRFGRRLSLYVNTAAEAIRAL
                     SMQMPGFRRQMNEGWYQIRIAGYDTAPEAVYARLHEQLGEGTVIHIVPRLAGAGKGGL
                     QIVLGAAAIVGSFFTAGASMALWGSALAAGGFSATTMLFSLGASMILGGVAQMLAPKA
                     KTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGGGGK
                     VVVIGRQG"
     gene            complement(10607..11350)
                     /locus_tag="ACHURS_002549"
     CDS             complement(10607..11350)
                     /locus_tag="ACHURS_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR1238246.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVISTPEGERYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGDIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGTEMPDFHREDDWWRNGQNLYLDNMAVT
                     GFYRVPLSSAQAGDILLCCFGASVPNHAAIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSVWRHRHWHASAFTGIYNDLAAASACM"
     gene            complement(11361..12059)
                     /locus_tag="ACHURS_002550"
     CDS             complement(11361..12059)
                     /locus_tag="ACHURS_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFR1238247.1"
                     /translation="MQDIREESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGRKYEAYPIDGSGFEMNGRGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSQLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCELRRNV
                     GNFGGFLSINKLSQ"
     gene            complement(12059..12388)
                     /locus_tag="ACHURS_002551"
     CDS             complement(12059..12388)
                     /locus_tag="ACHURS_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1238248.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            complement(12385..14958)
                     /locus_tag="ACHURS_002552"
     CDS             complement(12385..14958)
                     /locus_tag="ACHURS_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001621422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR1238249.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMSRVRRHFSGLDTDARKTASAV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLADAITLPMVGVTSLAVATGALVYAWYQGDSTLSAFNKTLVLSGNQS
                     GLTADRMLTLSRAGQAAGLTFNQARESLAALVNAGVRGGEQFDAINQSVARFASASGV
                     EVDKVAEAFGKLTTDPTSGLMAMARQFRNVTAEQIAYVAQLQRSGDEAGALQAANDIA
                     TKGFDEQTRRLKENMGTLETWADKTGKAFKSMWDAILDIGRPESSADMLASAQKAFDE
                     ADKKWQWYQSRSQRRGKTSSFRANLQGAWDDRENARLGLAAATLQSDMEKAGELAARD
                     RAERESSQLKYTGEAQKAYERLLTPLEKYTARQEELNKALKDGKILQADYNTLMASAK
                     KDYESTQKKPSGVKVSAGERQEDQAHAALLALETELRTLEKHSGANEKISQQRRDLWK
                     AENQYVVLKEAATKRQLSEQEKSLLAHEKETLEYKRQLAELGDKIEHQKRLNELAQQA
                     ARFEQQQSAKQAAISAKARGLTDRQAQRESEEQRLREVYGDNPAALAKATSALKNTWS
                     AEEQLRGSWMAGMKSGWGEWAESATDSFSQVKNAATQTFDGIAQNMAAMLIGSEQNWR
                     GFTRSVLSMLTEIFLKQAMVGIVGSIGSAIGGAFGGGASASTGTAIQAAAANFHFATG
                     GFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMR
                     RAEGINFNQNNHVVIQNDGPNGRAGPQLMKAVYEMARKGAQDELRLQLRDGGMLSGSG
                     G"
     gene            complement(14939..15352)
                     /locus_tag="ACHURS_002553"
     CDS             complement(14939..15352)
                     /locus_tag="ACHURS_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFR1238250.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRRFYRTHYFQDTQLDMHFSGLTY
                     AVLSLFFCDPDMHPSDFSLLVPRHEEEQVERPDEDKMLMQKAAGLAGGVRFGGDGGRD
                     ILSSADVADVMVDDAALMMASAGIPGGVRYVPAGW"
     gene            complement(15379..15810)
                     /locus_tag="ACHURS_002554"
     CDS             complement(15379..15810)
                     /locus_tag="ACHURS_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFR1238251.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEQAESSGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTQSPSMNEAVMKIEQEVLTTWPADAIARAED
                     VVLCLSGMIEAVRPDTDITEVAKNNTLTDDDFSAGKSSTAS"
     gene            complement(15824..16576)
                     /locus_tag="ACHURS_002555"
     CDS             complement(15824..16576)
                     /locus_tag="ACHURS_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1238252.1"
                     /translation="MATPNPLEPVKGAGTTLWVYNGKADAYANPLSDDDWQRLAKVKD
                     LTPGEMTAESYDDNYLDDEDADWSATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFES
                     GDVRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSKITP
                     VSAIKVTPTSGTVAKGKTTTLTVSFEPESATDKTFRAVSADPSKATISVKDMTITVNG
                     VATGKVQIPVVSGNGQFAAVAEVTVTEAGAAG"
     gene            complement(16584..16979)
                     /locus_tag="ACHURS_002556"
     CDS             complement(16584..16979)
                     /locus_tag="ACHURS_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFR1238253.1"
                     /translation="MKHTDIRAAVLDALEQHEHGATLFDGRPVVFDEEDFPAIAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDQWMESRIYPAMTAIPALAGLITTM
                     VTQGYEYRRDDDMALWSSADLTYSITYEM"
     gene            complement(16976..17551)
                     /locus_tag="ACHURS_002557"
     CDS             complement(16976..17551)
                     /locus_tag="ACHURS_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1238254.1"
                     /translation="MAIKGLDQAIDNLNRVRKNAIPAASAMTINRVATTAINQSSSQV
                     ARETRVRRKLVKERSRLKRATVRNPNARIIVNRGDLPVIKLGIRMLGRRPNSILKAGQ
                     HRYQRAFIQRLNNGRWHVMQRLPQARYEEGNDDKGRKKRNRLPIQVVKIPMAAPLKQA
                     FDENVDRIRRERLPKELAYALKQQLRIAIKR"
     gene            complement(17566..>17639)
                     /locus_tag="ACHURS_002558"
     CDS             complement(17566..>17639)
                     /locus_tag="ACHURS_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFR1238255.1"
                     /translation="PDDGGSCYLWLNRGQPPAVNRRR"
BASE COUNT         3828 a         5207 c         4258 g         4346 t
ORIGIN      
        1 acaattcttg gcgatggagt tgtgcacaat agcttcggtc aaaaactgat gcgcatttac
       61 aatcagaaag gcatcttttc aaacacaaaa gattccgaag aaggcttaac acatatactc
      121 tcagagcatt tcgagaatgt taaaaccagg gttcaaggta ctgtagtaat gttttccgct
      181 tcagggaaaa aatagcatcc aaccgcagca cgttcttgct taagacgtgc tgcggcataa
      241 tcccaatgat tactccctga cagggttcgt aggccactca atatcaggtg cagttgatgt
      301 atcaacacgg ttcagcaaca cccgatactt tttctaggct tccaccacca gcacgacaag
      361 atgccgcata cagtgaccca gtcagtccag ttttcagaca accagtgcgt cacctttttg
      421 aaggcgcttt aaagcacgtt ttaatccagg tcggcctgtc cttgttccgc ttaatttatc
      481 ttcaaatatt tgttcatatc ctgcacaaac aagagcgttt cgttgcaggt ctgtattctg
      541 gtcatttgtt gataccctta catagccaat cagcacgctg aatctcccgt ccaaaagcac
      601 aaatcatgcc atgcaggcca gaaaccgcca ttatctaaaa cctcggttta cgagaactcg
      661 gcacaagcgg ggagaaaata ccgttactca gtacagcgaa tacctggact aatcgacaaa
      721 cattcagcgg tggcctttct ggtgaactat ccggcaatgc ttctacagct gcaaaattaa
      781 aaactgccag gaaaataagc aatgtggctt ttgatggttc ctccgatatc acattaaaag
      841 caagtcatgt tggtgcgttt gccttaggga aaacaggaag caccgttgcg aatgataaag
      901 cagttggatg gaactggagt agcggagcct ataacgcaac tattagtggt gcatcaacgt
      961 taattattca tttttatatg ggagaaggaa gttgtcctgc agctcagttt cggattaatt
     1021 ataaaaatgg cggtattttt tatcgttcag cccgtgatgg ttatggtttt gaagccgact
     1081 ggtccgaatt ttacaccacc agaaaacctt cagcaggaga tgttggtgca ctgccgttat
     1141 ctggtggtca actgaatggt gcactgggta tcggaacatc cagtgctctt ggcggtaatt
     1201 cgattgtatt gggtgataat gacacgggct ttaaacaaaa aggcgatggt aatctggatg
     1261 tttatgctaa taacgtccat gttatgcgct ttgtctccgg aagcattcaa agtaataaaa
     1321 ccataaatat tacgggacgt gttaatccct cggattacgg taactttgat tcccgctatg
     1381 tgagagatgt cagacttggc acacgagttg tttagaccat gaagaaaggc gtgatgtatg
     1441 aaaaatcagg ccatgtaatc acggggcttg gtattatcgg tgaagtcgat ggtgatgatc
     1501 cggcagtatt cagaccaata caaaagttaa ttaacggaac atggtataac gtatcgcagg
     1561 tataatcatg cagcatttaa aaaacattgt cgcaggtaat ccaaaaaccg ttgaacaata
     1621 ccagctaaca aagaattttg atgttatctg gttatggtcc gaagatggaa aaaattggca
     1681 tgaggaatta aaaaactttc aggaggatac aataaaactg gcttacacag ttgagggaat
     1741 aattattgct atagacaagg atgtatcagc aattaatcca gaaggtttaa gtgtcgttga
     1801 gttgcctgat attacagcaa atcgccgggc tgatatttcg ggcaactgga tgttcaaaga
     1861 tggtgcggtg ataaagcgaa cttataccga ggaagagcaa aggcaacaag cggaaaatga
     1921 aaagcagagc ctgctgcagc tcgtcaggga taaaacccag ctgtgggact ctcagctaca
     1981 gctgggcatc atttccgatg agaataaaca aaaattaacc gagtggatgc tctatgcgca
     2041 gaaggttgaa tccacagaca cctccagcct gccagtaacg tttcccgaac aaccagaatg
     2101 agagaaggcc cgctatcggg ccttaatttt tattcaggct tttgtggcca ttcaggattt
     2161 gccgtatcca cacggctgac cagaacactg tagcgttccc atgcttccag tcgtgtgcgt
     2221 tcctcgtctg ttgccatatt cagcctgaca gcgcgttcca gcggctggat gactgattca
     2281 gcttcggaaa gcaatgcggc cttttgtgat tcggcctgtt gttgcagttc ctgttctgta
     2341 tattccctct ttactacttt accatccaga aacatccatt ttccgttatt gtctgcgtga
     2401 cgatttgccg taatatcagg aacttcaact atgcttagcc cttcaggatt aatagttgaa
     2461 acgtctttac tgacagcaac gatgatgttg ttctcatcgt aagcaatttt tatggtgtct
     2521 ggctgaaaat tcttttgttc ctcataccag tttttgccat cttcgcaaaa gagccatacc
     2581 acgcccgctt ttttagttag ctggtattgt tccggtgttt tcggattccc ggccttaata
     2641 tttttcaggt gcatcatctt aaacactccc cacgttgtac catattcccc caatcaattt
     2701 ctgaattggc cttctggcaa cagtgtcgac aatatcatcc gcattgctgt taattgcggc
     2761 tgttaaaaca taacctgacg catcgccaaa gccgtagtcg cgccatacgt ttttatattc
     2821 aatgctgcca agacgaatat cccgaacgac atgattatgc agccatgtgc ttaaccagtt
     2881 gttttcccac actgaaccat aaatatcgcc actagacgca acttgcgcac cacttcccag
     2941 cgcaaatccc ccatctgttt taaaaatgaa atcaccacca ccatctgaac cgttattaat
     3001 atgaacacca tcaccaccgt tatctttcca caaaaacata cgaattcttc cgtcttcgtt
     3061 ggcgaatgta atgtggtggc ggtaattact ttttaatcta aaattgctgc cattagattc
     3121 aatatcttta taaagatgta atcctaactc atcaatatgt ccgatgcgcg aaccatttga
     3181 atagaagcta ataataccat ctccatcttg cctaaatccg gtgtcgttat ctcccagaac
     3241 aatggagttt tcacccagtg cgttatcagt agctccaata gctagacctc ctttaatttt
     3301 ggccccgtgg ctgacagata tagcacctgt tctcagattt atagcgaatg gccttaatgg
     3361 gccgatatta ccgttttcgc cttgtccttc agccgtcgga atgaaatgaa gaaaatcttc
     3421 tgaacgacgg aaaattagac cgaaggtatc attgaaaatt cgcagcgcat tcaccgtgcc
     3481 aattttcagt tctccggtca ttttatcgcc ggaacgctga acggcgttac cagccttgtt
     3541 taccgtttcc tgtaaaccga ggtattcgat aacggcagca acggtcgatt tcgcaagaat
     3601 atcccgcccg acttttgtca gggttgccag gctggcgaca tcattccccg taaaatacgg
     3661 aaacttgcct gccgcagtag taagtccggc cagcgccgtc agggtggcat ctttcggttg
     3721 cttacccgca agcgcgttag tcatggtggt cgcaaaattc gggtcgttgc ccagcgccgc
     3781 agccagctcg ttcagcgtgt tcagtgcatc aggtgacgaa tctacaaggg cggcaatcgc
     3841 agccataacg aaagccgtgc ttgcgatttg ggtattattc gttccctgtc gcgcagttgg
     3901 tgttgttggc gttccggtca gtgcaggact atttaagggc gctttcttgt tcgtttcacc
     3961 cattaccgcc ttaaccgctt ttggcgttgc cgccagtgac tcggaagtgc tgttggtcgc
     4021 actgctgagc tgtactatcc cctttttcgt cgtgctcgca tcctccagcg ccacggcgga
     4081 tgcaatatcc tctgcccgtt ttgctgctgt ctcggcgcgc gttgccgcgg attcagcagc
     4141 aactttgctc tgagatgcag ccgtcgcact gcctgccgcc tctgatgctt tcgttgttgc
     4201 tgtcgtggca ctacctttcg ctgctgacgc ttgtctggtc gcctcatctt ttgaagcaga
     4261 cgcagatgat gccgatgacg ccgctgaact ggctgacgat gcggctgctg ccttagagga
     4321 agcagcattg tctgctgaag tctttgcatt tgtttcagag gtttttgctg cagaagcaga
     4381 cctcgctgct gcagtggctt gctcagtggc tttgccagcc ttcgttgtgg ctgttgaagc
     4441 ggatgatgcg gcgctttctg ccgattttcc ggcggcggtg gcactggctg aggcctgccc
     4501 ggcacttgtt gacgcggcac tggcagataa tgcagccgct gtttttgaac ctgccgcagc
     4561 tgaggcgctc tgtcccgctg ctgtttcaga agacttagcg ttcgtctcgg acgtttttgc
     4621 cgccttcgcg gaatttcctg ccgccgttgc cgaggaagct gcactactgg cacttgatga
     4681 tgcattcgtt tctgaagatt tcgctgcctc ttttgaggcc gccgcacccc gtgccgaggt
     4741 ggcagcttct gacgccttcg tggtcgctgt ggatgcagaa gtggctgccg atttttgtga
     4801 tgctgcggca ttcgtttctg acgttttcgc ggcactggcg ctggtagctg ccgcgctttt
     4861 tgatgactct gcagcagcac tttttgctgc ttcacgggcc tttgtcgatg ccgttcctgc
     4921 gctggaagac gctgactgag ccgacgacgc ggcctgtccg gctgacgtgc tggcggcacg
     4981 tgctgaggct gcagcatcgg ttgcatgagt tgccgcctcg ctggctgatt ttttcgcggc
     5041 tgccgtattc tgtgcaaccg cggaggcgtt acgtgacacc tcttccacca tctgctcaaa
     5101 gcggcgcagt gcctccggtc ggacatcatc ctccgtcatg gcaccgagaa aatcattcag
     5161 cgtacctggt ctggaacctt catagacggt aatggtcccg gcatgtgaag gcggaaaacc
     5221 ttcaaccagc agggtgacgc tgtactgacc atgctcaaca tccatgctgt aacgtccggc
     5281 ttcatccgga ttttcagagg ccaccgtgtt caccaccatc gtgctgctgg ttcgtctggc
     5341 cttcagcaca atggtgcagt tctgtactgg ttttcctgtg ccatctttaa gcacgccaga
     5401 aatttttact gtcatacttt tccaccaata aaaaaagccc gcagcagtga cgccacgggc
     5461 ttcaggacag tgtaacttta cgtttcctca aacgcagttc accccataag gtggatgaac
     5521 ctgcgtatca taacaatatt tacagaagat aaatcggcgt ctgttgtcag atacggtatc
     5581 cgataccaac aataaatgca tccgttcgcc agtcaccact accggaacct tcataagcaa
     5641 ggtcaatggt cacggattcg gtcgggttaa actgcacgcc agccccccac gccagagacg
     5701 tgttgctgtg gcgaccgtca tcacttccgg tcagcacatc gtgcgttttc cccttgttgt
     5761 cagttacgcg gagataatcc ccggagaaag tcgacacacg gctgtaagcc acacccacca
     5821 tcgcatacgc gctgaaccat tcattcacgc gtacagacgg ccccgccatc acgctgaacc
     5881 agcggttacg cacggaatct tcatgccagc gggtatcgct gtagtgcgtt ttttgctcat
     5941 cctcagcatt ggcataactg aaggacgtaa tcagccccag cgcgtccgta aactcataac
     6001 ggtatttcac gttaatcccg ttcagattat cgctgccggg agcgttcgta cgggcatgaa
     6061 gataccccgc gctcagtgtg gactgatgtt cagacgccca tgcaggcgca ccggatacgg
     6121 acagacagat ggctgcggac aaaatggctg cacaaacttt acgcataatt acctctcgct
     6181 tttctgcaat aaaaaaggcg ccatttctgg cgcccgtata tgggttataa aattcagctg
     6241 atactgatgc ctgcggtggc tttcttcatc accacaacca gcaaatcgct gatacttgct
     6301 gtgggatacc agttattcac cagccatgct gacaccgaaa actccagcgt catgtggccg
     6361 cgaccagctg gcatatcaat aacaccactg taaatcagcg tattatccag cgcggtacgg
     6421 ttataaattt cagcaccgtt tttccgtact atcaggcggc atgacgaata aatatcgtta
     6481 ttctcccgct catgtttagc gccgctgaat gccaccgccg gaataacaat ttgcctgtca
     6541 aaaggctgat cgtcataaat cctgacggta atggtccctg atggccaccg ctccggtgcc
     6601 cgggagtccc gcggaaaagc cttacccact gttttgacta tatcgccttc aatctggttg
     6661 gctgacagtt tccccttaat ctgacagttc tcattaattg tgacattgtt gagcgtcccg
     6721 gcgttcgcat tcacactgcc actgatatct gcatttttag cggtcagttt tccgtccggt
     6781 gtcagggaaa atgccggagg attaccgccg ctggtaatgg tgggagccgt caggcgtttc
     6841 aggaacacgt cgttcatgaa tatctgatcg ccctgaccaa caaacatcgg ctttgtgttg
     6901 ccattcgcag gattaatcat cgcaatcctg tctgccgcca gcagcacctg actctgcatt
     6961 cctgctggcg tattctcaat accggcaccg atacccgcaa tataaaggcg tccgtccttc
     7021 atctgttgca gcttcacagc ccacatgctg ttcagattat tatttgtatc aacctgaacc
     7081 ttctgtatct gctggattgc cgcactctgg tctttcagtt tcttattgac agtctgcgtg
     7141 atttcattgc tgacatccgt aatggacgtc ctgatttcag ccaggtcagg cgcaagctga
     7201 ccgttatcaa tctgcgtcca cagctcctga gccagatgtg ttttccctat ctctcctttg
     7261 aaaaaatcca agtaaccttc cgcatcatcg ctcgcccgac cgacagcctc cacgaatgcc
     7321 gatttgccaa cggtgttcac actgcgaacg taaaaataat aatcatggcc cggtttgata
     7381 ttgatactgg cggctatcca gtacagcgcc gtgccaagat agcgggcgct ggtttcaacc
     7441 tgcctgatat ccgcaatccg cttttccgag aaccagaact caaactgtac cgtcgggtca
     7501 taaacggcaa gatgcggcgt tgcggttatc tgaaaatagc ccggcgtcag ctcaatccgc
     7561 gacggtgctg ccggtgcggc aatccggaag gtggtggtgg caggttcacc ctgctggcca
     7621 tagctgttta tcgcccgcac cgtcagggtg tattccccga gcggcaggcc gctgaaacgg
     7681 tgctccgtgt ctgcggtgat ggcggtggtc accagtctgg catccgttcc cttaccactg
     7741 gtcaggcgca gactgaagcg cacacccttc accacccgcg gcgtgtccca tttcgcctgc
     7801 gccagatact ggccgtcagg tgctgcactg ccggtgggat gacgctgttc agggaacctg
     7861 actgcggctc aaagcgggca ccgttatcca cgatggcttc tttttccagt acgtgctgca
     7921 ccgccgtgat ggcaaaggtg ccgtccgtgt tttcccggac ggagacacag cggaacagga
     7981 gacggcgcag tgacggcagg gagagtcccc acaccccgta tgtctccaca ccatcaggca
     8041 gggtactgac ctgtatccgg tccggcgcgg ggtgttcggt gatgtccaca ctcaccggct
     8101 taccgctgcc gttaatcagg ttcaccgtgg cggcaccggt ctccggaagt gtcacttcac
     8161 ggtccagcgt cagagtgcgg gtggcagcat caatggacag gacacgtccg ccggtcaggg
     8221 tcccggcata gtcgttatca cagatttcaa tgatgtcacc gggtgtgtgc cgcagcccct
     8281 gagacccgag cgtgaaatcc accgtctgcg tttccagcag ttcggtcttt atcacccaca
     8341 gtccggcacg gtgggcctga ccgcggctgg tacagccgaa cgcgtccatc ttcagcaggt
     8401 tgcgtccgta gcgcagtatg gcttccgggt cttccaccag ttccgtggag gtctgccagc
     8461 cgttctgcgg gtcggtataa ttcacctcca ccgccgtgtg ccggtccttc agggcactga
     8521 agctgtagcg gaaacccacg ccgttatcat ccaccaccac atcgctgttg gtgtacggcc
     8581 acaccacatc cgacgggcgg tcctgaacaa acgtcagcgt ctggccgttc cataccggca
     8641 tacagcgcat cgccgagcag aaatcactga gcacgtccca tgccttacgc tgttgtgcca
     8701 ggtacgcatt gaaggtcatc cgcggctcgg tctccccgaa actatccggg accgtctggt
     8761 cgcagtactg cccgatggca tacagcgccc acttgtccac atccgccgcc cccagacgtt
     8821 ttcccatgcc gtagcgcggg tgagtcagca tgtcccacag acaccaggcc ggattgttgc
     8881 tgtatgccgg tttcaggcta ccgtcccaga taccactgta cgtgcgtttt tccgggtcat
     8941 agtttgacgg cacctggatg atgcgaccgc ggatatggta gttcaccgtc atctgctggc
     9001 cgccgaactg ctccgcatcc acctgcagcc ccacaatggc cgtgttcggg tagcactgtt
     9061 tcacatcgat gatttcggtg tatgacgacc acagcgtctt attctgcagc tggtccgtgg
     9121 tgctgtccgc tgtctcccgg accatccgga tgttaaaagg acggggaggc agattatcca
     9181 gaatcaccga cgccagaaac tgcgaggtgg tcttgccgtt aatggtgaca tccttttctg
     9241 tcacccagtt accgttacgc tgtaactgaa tcagcaggcg gacggatgtc gggtttcggt
     9301 caccctttga cgtggtctgc accagtgact gcaccccgaa ggtaacccgc aggcggtcaa
     9361 tgttcgcgga cgtaatggtg cgcgtcaccg gttttgcctt cgtcacttcc acgcccagtc
     9421 cggtttcagc accggaggac tcaaagcctt ccggtggtgt ctgctcctgc tccccggcgc
     9481 gtcagaccgc cgtcacaccg tgtatcacgg gattaccgtc cgtgtccgtc agcggggttt
     9541 tgttcaccag gatactctgc agtcccttca ccggaccttc tatcagtccc tcaccaatcg
     9601 catcaatcac gctcatcatc tgcgtggatt tgagattatc cttcgcctca cgaggcgtgt
     9661 gtgccttacc gccacctttt cccatacagc cttcccctga ataaattaac cgccacttgc
     9721 cattccgtac agaagtcgga tatccttcgc ccgaaaagca tgaaacacat ttctgccatg
     9781 ctaaagagaa accccggtat cagcagatac cggggttttc tttcatgccc accgataatc
     9841 ctgttggtta aaaccggtaa tggcataaaa attctgaata tcttcacatt ttcacaaact
     9901 gactgtggcg cgtataattt ctctgcgtta atttttttgt cgtgatataa gaataattcc
     9961 ttacacttaa tcttcgtaac tctcccgcag ttcctgtccg cgatcactgc gggatttttt
    10021 tattcttttt acccctgccg cccgataacc acgacctttc cgcccccgcc ttcatcacgg
    10081 gtgctgatgt cctgggatat acggcgggag ccaaccagca tttccccgta aggcaccggc
    10141 atcgggttcc cctgggcaat catgttatcc agtgaggaaa agtacgtgtt ctgtctgccg
    10201 ttatccgttg cgcggtaatc cggtgttttt gccttcgggg ccagcatctg ggccacaccg
    10261 cccagaatca tgctggctcc aagtgaaaac agcatcgtgg tggcagaaaa accaccggct
    10321 gccagggctg aaccccataa cgccattgat gccccggcag tgaagaaaga gcccacgatg
    10381 gctgccgccc ccagcacaat ctgcagtcca ccctttccgg ccccggccag tcgcggcaca
    10441 atgtggatga ccgttccctc acccagctgt tcgtgaagac gggcgtacac cgcctccggt
    10501 gccgtgtcat aaccggcaat acgtatctgg taccagcctt cgttcatctg acggcgaaag
    10561 cccggcatct gcatcgacag ggcgcgaatg gcttccgctg ccgtgttcac atacaggctg
    10621 aggcggcggc caaatcgttg taaatccccg tgaaggcaga tgcgtgccag tggcggtgac
    10681 gccagactga atgcgttcgt cgttgccatt tttcggaata cctctcccgt ttactcagtt
    10741 gttcaggcag atggtgaagc agctcaccgt tgccgcagta aatggcggca tggttcggta
    10801 ccgaagcacc aaagcagcac agcagaatat cgcccgcctg tgcagaggac aggggcaccc
    10861 ggtaaaagcc ggtgaccgcc atattgtcca ggtaaaggtt ctgaccgttg cgccaccagt
    10921 catcctcgcg atgaaaatcc ggcatttcag tccccgccag atgataagca tcccggaaca
    10981 gcgtgtaaca gtccgtcacc ccgtgctcaa agcgccgtcc tgtcagatgt ggcacacagc
    11041 ggaatttgtg aatgtcaccc cggcagacca accaccaggg cagtgcgctt tttatctgca
    11101 accgccggtc agcctcgctc agccagggca gcccaccggg atgactgtgg accagtgcca
    11161 caatctcccc ctgcatctct gcccgcagcc agtcttccgg tgcgatacga aaatacgcct
    11221 ccggctctgc ggaaatattc acacaaggga tataccgctc cccctccggc gtgcttatca
    11281 cgaagccgca cgactccgca ggcgcacacc gccgggcatg tgccagaatc gctgattcag
    11341 tctgtgtcat aaaccgggat ttactgcgaa agtttattaa tggaaaggaa accgccaaaa
    11401 ttgccgacat tcctgcgcag ttcacacccg cgcatgcact tgctgcatct gtccttacgg
    11461 atatccgtgg tggggttgtc gaactcatcc gccaccgcag gaccgttata cccgcattca
    11521 tctccccggt aatcccacat acaggtgttc gccagcatga tgcgaccggg aaacagcgcc
    11581 ccgtccgtct cggtcggtgt agccagcaca aacgaggccg tcatggctgt cagctgcgac
    11641 atctgctcca ccacccagcg gtcactcagc tcctgctccg ggtccgcctc cggattgccc
    11701 gcaacgaaat tcaccgcatc cagaaaacgg gcatacaccc ggcggcggac caccgtggcc
    11761 cccaccagac tctgcaggtc ttccgccatc ccggtgacca gaccgaacag attggacacc
    11821 gtcagcgacg gtctggcact gctgccccgg ccgttcatct caaagccgct gccgtcaatc
    11881 gggtatgcct catacttacg cccctgccag gtgaccggct cccctttttc attcagctca
    11941 ttacagaaaa aataccgctc accaccctgt accgtcaggt cgatttccca gagtaccacc
    12001 cgcggtgact gctctgactt aaccgactcg ttcagacttt cttcgcgaat atcctgcatc
    12061 agttcaccac ctgcttaaac tccgcgctga actcaacgcg caacatcccg acccgcgcag
    12121 accacccggc acaggtcacc tttatctgcc ggtatgcata gggtggcttc cacaaaaatg
    12181 ccttccagcc accgtgctct gccaggaacg cttccagatg ccgggcctcc tcccgggtca
    12241 cggaaagcgt caccctgtat gttttcaggt cagcattcag ccctgccgcc atacgctgtg
    12301 agtacccgtc accaaaacgc acttcacgca ccgatggctg cgagttcacc tccatatccg
    12361 gcttcacttt ccagcgaaag gttttcatcc accgctccct gataacatac cgccatcacg
    12421 caactgcagc cggagttcat cctgtgcccc cttgcgggcc atctcataca ccgctttcat
    12481 cagctgcggc cctgcccgcc cgttggggcc gtcgttctga atcaccacgt gattgttctg
    12541 attaaaatta atgccttccg cccgccgcat ctgcgccgga cttccggcac cgccgacata
    12601 accaccttcc gcatagcccc gcatcaggcg gtacagattg ccgacaccaa tccggctggt
    12661 cgcctccttc gtgaagacaa actccccgcg atgaacaatc cccgcaggtt catatttacc
    12721 ccccgtcccc gtaaatcccc cggtcgcgaa atggaagttc gccgccgcag cctgaatggc
    12781 cgtccccgtg gaggcagacg caccaccacc gaaagcaccg ccaatggcgc tgccgatact
    12841 cccgactatc cccaccatcg cctgcttcag aaaaatctct gtcagcatgg acagcacaga
    12901 acgggtgaaa ccacgccagt tctgttcgct gccgatcagc atcgctgcca tattctgtgc
    12961 aataccgtca aaggtctgcg tggccgcgtt tttaacctgc gaaaaactgt ccgtcgcact
    13021 ttccgcccac tcgccccagc cggacttcat cccggccatc cagcttccac gaagctgctc
    13081 ctccgcagac caggtgttct tcagtgcaga tgtggccttc gccagcgcag ccggattatc
    13141 accgtacacc tcacgaaggc gctgctcttc cgactcccgc tgcgcctgac ggtcggtgag
    13201 tccgcgggct tttgcgctga ttgccgcctg cttcgcgctc tgctgctgtt caaaccgcgc
    13261 cgcctgctgt gccagctcat tcagccgttt ctggtgttca atcttgtcgc ccagctcagc
    13321 cagctggcgt ttgtactcca gcgtttcttt ctcatgggcc agcagggatt tttcctgctc
    13381 agataactgc cgtttcgtgg cggcctcttt caggaccaca tactgatttt ccgctttcca
    13441 taaatcacgg cgctgctggc tgattttttc attcgcaccg ctgtgcttct ccagcgtcct
    13501 gagctcagtt tcaagcgcca gcagggctgc atgcgcctgg tcttcctggc gctcaccggc
    13561 agacaccttc acaccggacg gctttttctg cgtcgactca taatcctttt ttgccgacgc
    13621 catcagcgtg ttgtaatccg cctgcaggat tttcccgtct ttcagggcct tattcagctc
    13681 ttcctgccgg gcggtatatt tctccagtgg cgtcagcagg cgctcatacg ccttctgcgc
    13741 ctctccggta tacttcagct gtgacgactc acgctcagcc ctgtcccttg ccgccagttc
    13801 accggctttt tccatatccg actgcagcgt tgccgctgcc agacccagac gggcattttc
    13861 ccggtcatcc catgcgccct gaaggttggc ccggaaagag gaggttttac cgcggcgctg
    13921 gctccgactc tggtaccact gccatttttt atccgcctca tcaaatgcct tctgcgcact
    13981 ggcgagcata tccgctgagg attccggacg accgatatcc agaatggcat cccacatcga
    14041 tttgaatgcc ttccctgttt tatccgccca ggtctccagt gttcccatgt tttctttcag
    14101 gcgacgggtc tgctcatcaa agcctttcgt ggcgatatcg ttcgccgcct gcaatgcccc
    14161 ggcctcgtct ccggaacgct gcagctgtgc aacatacgca atctgctctg ccgtcacgtt
    14221 acggaactgg cgcgccatcg ccatcagtcc cgacgtcggg tcagtggtca gcttcccgaa
    14281 ggcttcagcg actttatcca cctccacacc ggatgcagac gcaaaacgcg cgacactctg
    14341 gttgatggca tcaaactgtt caccaccacg cacaccggca ttcaccaggg ctgccagtga
    14401 ctctctcgcc tggttaaacg tcagccctgc tgcctgcccg gctcttgaga gagtcagcat
    14461 acgatcggca gtcagtccgg actgattacc ggaaagaacc agggttttat taaacgctga
    14521 aagcgtggaa tctccctggt accaggcgta caccagtgca cctgtcgcca ccgccagcga
    14581 ggtgaccccg accatcggca gggtgatcgc atcggcaagc cccctgaaca tggggatcat
    14641 cccgccgaag gagtccttca cctgaccgcc ctgttgcagc aggatcagcc agggattctg
    14701 accaccggca agctgcgtgg cgatatccgt aaactgtgcg ggcagggttc gcatggccgc
    14761 tttatactgc ccgacggaaa tcccggcttt ttgtgcagcc agcgcctggc ggctcaggcc
    14821 ctgttcaaca gcactggcgg tttttctggc gtcggtatcc agacctgaaa aatgacgcct
    14881 tacccggctc atctgctcat cgaaacggac agcatccaga ctcaggtcaa taacaagatc
    14941 accaaccggc tgggacatat ctcacacctc ccggaatccc cgctgaagcc atcattaatg
    15001 cggcatcatc caccatgaca tccgccacat ccgcagacga taaaatatcg cgccctccgt
    15061 ccccaccgaa ccggacgcct ccggcaagtc ctgccgcttt ctgcatcagc attttgtcct
    15121 catccggcct ctccacctgc tcttcctcat gccgggggac aagcagactg aaatcagagg
    15181 gatgcatatc cggatcgcaa aaaaacaggc tgagtacagc gtacgtcagc ccggaaaaat
    15241 gcatatccag ctgggtatcc tgaaaataat gcgtgcggta aaaacggcgc cagtcggcat
    15301 attcggtgga tgtcatcccg gcaagcatgg cgcgccagtc gggtctcccc atctcacgcg
    15361 ccagtctgag ggcaaagttc agctcgccgt cgaagacttt cccgcagaaa aatcatcatc
    15421 agtcagcgtg ttatttttcg ccacttcagt aatatcagta tccggacgaa cagcttcgat
    15481 catcccggac aggcacaaca ccacgtcttc cgcccgggca atggcatcgg caggccaggt
    15541 ggtgagcact tcctgctcta tcttcatcac ggcctcattc attgacggtg actgcgtttt
    15601 ctgtggatgg ttatgccaca gggacatcgc caccagaaac gcgccggttc tgacgagatc
    15661 ttccacgctt acctgcaggt tgccgctgga ttctgcctgt tctgcacgcc gtttcaggag
    15721 ggcaagatgc tcgatacgct gcagcgcaga caattcggaa agcgtgacag acacaccgtt
    15781 atattcaaat tgttctgttt tcagaaacat gtattacctc cgtttaccct gcagcgcccg
    15841 cttcagtaac ggtgacttca gccactgcgg cgaactgacc atttccgctc accacaggga
    15901 tctgcacctt acctgtcgcc acgccgttta ccgtaattgt catatctttc acactaatgg
    15961 tggctttcga cggatcggcg gaaaccgctc tgaacgtctt gtcggttgca ctttccggct
    16021 caaaagaaac cgtcagggtg gttgttttcc cttttgccac cgtaccggat gtcggcgtca
    16081 ccttaatcgc actgaccggc gtaattttgc tgcgttcttc cgctacagaa ggtttaccca
    16141 cgttagtgac tttcaccgtg cgggtgatca cttctttcgc cgtcacggcc ttaccgatac
    16201 tgctgaccca gccacgaaac acatccaccg tgccattcgg aaaacggatt ttataggccc
    16261 ggacatcgcc gctttcaaac cagcctataa gccctttctg gccttcctct cccggtttcc
    16321 aggccagcgt aaaactggta tcacctgcag atttctgccc ctgcccggtc gcgctccagt
    16381 ccgcgtcttc atcatccagg tagttatcat cataggattc agccgtcatc tcgcccggcg
    16441 tcagatcctt caccttagcc agtcgctgcc agtcatcgtc tgacaacggg tttgcataag
    16501 catcagcctt gccgttgtaa acccacagag tggtaccggc accttttacc ggctccaggg
    16561 gatttggtgt tgccatatcg tcctcacatc tcgtatgtaa tggaataagt cagatccgca
    16621 gagctccata acgccatatc gtcatcacga cgatactcat agccctgcgt aaccatcgtg
    16681 gtaatcagtc ctgccagtgc agggatcgca gtcatcgccg gataaatccg gctttccatc
    16741 cactgatcga gctccgaatc aggtacctgt gccggtaaaa acacctcaat atgcagtgtg
    16801 gcccgccagg tatctgcatc cagctcttca ccggtatact ctgcatccgt cagataaacc
    16861 gcgatcgcag gaaaatcctc ttcgtcaaaa acaacggggc gaccatcaaa cagcgtcgcc
    16921 ccgtgttcat gctgctcgag tgcatccagc actgcggcac gaatgtcagt gtgtttcatc
    16981 gttttatcgc aatcctcagt tgttgtttca gcgcgtatgc cagttcttta ggcaggcgtt
    17041 cacgccggat acggtcaaca ttctcatcaa atgcctgttt cagtggggcc gccatcggga
    17101 ttttcaccac ctgaatggga aggcgattac gctttttcct tcccttgtcg tcattgccct
    17161 cctcatatct ggcctgggga agacgttgca taacatgcca gcgcccatta tttaatcgct
    17221 ggataaatgc ccgctgataa cgatgctgac cggctttgag tatgctgttc ggacgacgcc
    17281 ccagcattct gatccccagc ttaatcacag ggagatcacc gcggttaacg ataattctgg
    17341 cattcggatt tctgaccgtc gcccgtttca gtctggaccg ttcctttacc agtttccgtc
    17401 tcaccctggt ttcccgggca acctgtgacg aagactgatt aatcgccgtt gtggccacgc
    17461 ggttaatggt cattgcagaa gcagccggaa tggcgttttt acgaacccgg ttcagattgt
    17521 caatcgcctg atcaagccct tttatcgcca taattcaccc tgcgtttatc gtcgccggtt
    17581 aactgcgggt ggttgcccac ggttgagcca gagataacag ctgcccccgt catccggag
//
LOCUS       DAZPDU010000086        17447 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZPDU010000086 DAZPDU010000000
VERSION     DAZPDU010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17447
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.087"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..501)
                     /locus_tag="ACHURS_002559"
     CDS             complement(<1..501)
                     /locus_tag="ACHURS_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1238256.1"
                     /translation="MALTDIKVRTAKPTDKQYKLTDGGGMHLLVHPNGSKYWRLQYRY
                     EGKQKMLALGVYPEITLADARIRRDEPRKLLANGVDPGDKKKSDKVEQSKARTFKEVA
                     IEWHGTNKKWSEDHAHRVLKSLEDNLFAALGERNIAELKTRDLLAPIKAVEMSGRLEV
                     AARLQQR"
     gene            complement(687..771)
                     /locus_tag="ACHURS_002560"
     tRNA            complement(687..771)
                     /locus_tag="ACHURS_002560"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(735..737),aa:Leu,seq:caa)
     gene            967..1986
                     /gene="ahr"
                     /locus_tag="ACHURS_002561"
     CDS             967..1986
                     /gene="ahr"
                     /locus_tag="ACHURS_002561"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFR1238257.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYEPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKTLAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRNGKARYRVV
                     LKADF"
     gene            complement(1990..2553)
                     /gene="idnK"
                     /locus_tag="ACHURS_002562"
     CDS             complement(1990..2553)
                     /gene="idnK"
                     /locus_tag="ACHURS_002562"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFR1238258.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKQYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPQADEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQDRICAKGGSASDQRCE"
     gene            2770..3801
                     /gene="idnD"
                     /locus_tag="ACHURS_002563"
     CDS             2770..3801
                     /gene="idnD"
                     /locus_tag="ACHURS_002563"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HFR1238259.1"
                     /translation="MQVKTQSCVVADKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTDMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQADELQGKRVFVSGVGPIGCLIVSAVKTLGSAEIVCADVSPRSLSLAKEMGADVL
                     VNPQNDDMDHWKAEKGHFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLKGSFRFTSEFNTAVSWLANGVINPLPLLSAEYLFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            3825..4589
                     /gene="idnO"
                     /locus_tag="ACHURS_002564"
     CDS             3825..4589
                     /gene="idnO"
                     /locus_tag="ACHURS_002564"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFR1238260.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGYLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            4654..5973
                     /gene="idnT"
                     /locus_tag="ACHURS_002565"
     CDS             4654..5973
                     /gene="idnT"
                     /locus_tag="ACHURS_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HFR1238261.1"
                     /translation="MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGQYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            6040..7038
                     /gene="idnR"
                     /locus_tag="ACHURS_002566"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             6040..7038
                     /gene="idnR"
                     /locus_tag="ACHURS_002566"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HFR1238262.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSVHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLDKRVRRKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRMAAQMLLSKIKNNDHNHNTVDLGYQIYHGNTL"
     gene            7116..8618
                     /locus_tag="ACHURS_002567"
     CDS             7116..8618
                     /locus_tag="ACHURS_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFR1238263.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAEHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(8737..9819)
                     /gene="lptG"
                     /locus_tag="ACHURS_002568"
     CDS             complement(8737..9819)
                     /gene="lptG"
                     /locus_tag="ACHURS_002568"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFR1238264.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVASQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(9819..10919)
                     /gene="lptF"
                     /locus_tag="ACHURS_002569"
     CDS             complement(9819..10919)
                     /gene="lptF"
                     /locus_tag="ACHURS_002569"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFR1238265.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVVLDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(10982..11032)
                     /gene="ytgA"
                     /locus_tag="ACHURS_002570"
     CDS             complement(10982..11032)
                     /gene="ytgA"
                     /locus_tag="ACHURS_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFR1238266.1"
                     /translation="MSIIILATISTTQENK"
     gene            11186..12697
                     /gene="pepA"
                     /locus_tag="ACHURS_002571"
     CDS             11186..12697
                     /gene="pepA"
                     /locus_tag="ACHURS_002571"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFR1238267.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            12831..13274
                     /gene="holC"
                     /locus_tag="ACHURS_002572"
     CDS             12831..13274
                     /gene="holC"
                     /locus_tag="ACHURS_002572"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFR1238268.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            13274..16129
                     /gene="valS"
                     /locus_tag="ACHURS_002573"
     CDS             13274..16129
                     /gene="valS"
                     /locus_tag="ACHURS_002573"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFR1238269.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(16185..17381)
                     /locus_tag="ACHURS_002574"
     CDS             complement(16185..17381)
                     /locus_tag="ACHURS_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFR1238270.1"
                     /translation="MAQVINDMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLIGCV
                     LTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMALGMGTV
                     FFISTKMLHANSSSSVIISVVLMTIVCIVFIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTTYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGIM
                     PSLPFL"
BASE COUNT         4483 a         4336 c         4454 g         4174 t
ORIGIN      
        1 gcgctgttga agacgagcag ccacttcaag acgtccagac atttctacgg ccttaatagg
       61 tgctaataaa tctcgagttt ttaactcagc gatattacgt tcaccaagcg ctgcaaaaag
      121 attatcttcc agacttttta gcacacgatg ggcatgatct tcagaccact ttttattggt
      181 gccatgccac tcaatcgcga cttctttaaa ggttcgtgct ttactctgtt caaccttatc
      241 acttttcttt ttgtctcccg gatcgacgcc attcgcaagc agcttacgtg gctcgtcacg
      301 acgtattctg gcatccgcga gtgtgatttc aggataaacc ccaagtgcca gcatcttttg
      361 ctttccctca taacggtact gcaaacgcca gtacttagaa ccatttggat ggacaagcag
      421 atgcataccg ccaccatcag tcaacttata ttgcttatct gttggcttag ctgttctgac
      481 tttgatgtct gttaatgcca tgcgtattcc ctccctgttg gtacaagcat tatcgaaccg
      541 gaaataccat catctgtacc aacatatgtt ggtagaagta cgttgatatc ggttgacctt
      601 gagagagtta atatagcggg aaaggtagat aaatactgaa tttcaggcat aaaaaagacc
      661 tcagttgagg tctattgaca tacttttggt gccgaaggcc ggactcgaac cggcacgtat
      721 ttctacggtt gattttgaat caactgcgtc taccgatttc gccacttcgg cactgaagag
      781 gtatgcggaa aacgttgtgg attatacctg tcgcacgcca ccatgcaagc gccagcttgc
      841 tcgcacctcg ctaagtgctg aaattttcag cattgcatac agcgatgtgt aacctttgtc
      901 acactccagg caccccgccc tgccatgctc tacacttccc agacaacacc agagaaggac
      961 caaaaaatgt cgatgataaa aagctatgcc gcaaaagaag cgggcggcga actggaagtt
     1021 tatgagtacg agcccggtga attgaagcca caagatgttg aagtgcaggt ggattactgc
     1081 gggatctgcc attccgatct gtcgatgatc gacaacgaat ggggattttc acagtatccg
     1141 ctggttgccg ggcatgaggt gattgggcgc gtggtggcgc tcgggagcgc cgcgcaggat
     1201 aaaggtttgc aggtcggtca gcgtgtcggg attggctgga cggcgcgtag ctgcgggcat
     1261 tgcgacgcct gtattagcgg taatcagatc aactgcgagc aaggtgcggt gccaacaatt
     1321 atgaatcgcg gcggttttgc cgagaagttg cgtgcagact ggcaatgggt gatcccactg
     1381 ccggaaaata tcgatatcga gtccgccggg ccgctgctgt gtggtggtat cacggtcttt
     1441 aaaccactgt tgatgcacca tatcactgct accagccgcg ttggggtaat tggtattggc
     1501 gggctggggc atatcgctat aaaacttctg cacgcaatgg gatgcgaggt gacagccttt
     1561 agttcgaatc ctgcgaaaga gcaggaagta ctggcgatgg gtgccgataa agtggtgaat
     1621 agccgcgatc cgcaggcact gaaaacactt gcggggcagt tcgatctcat tatcaacacc
     1681 gtcaacgtca gcctcgactg gcagccttat tttgaggcgc tgacgtatgg cggtaatttc
     1741 cacaccgtcg gtgcggtcct cacgccgctg tctgttccgg cctttacgtt aattgcgggc
     1801 gaccgcagcg tctctggttc agcaaccggc acaccttatg agcttcgtaa gctgatgcgc
     1861 tttgcggccc gcagcaaggt tgcgccgacc accgaactgt tcccgatgtc gaaaataaac
     1921 gacgccatcc agcatgtgcg caacggtaag gcgcgttacc gcgtggttct gaaagccgat
     1981 ttttgaaaat cattcgcagc gctgatctga ggcgctaccc cctttcgcac atattctgtc
     2041 ttgtcgtatc gccagcacgg cctgccgaca ttgttcggtg acattggcaa tatcatggtt
     2101 gatatcaatg cgtacaatat cctgttcatc tgcttgtgga cgctccagcg cctcaaactg
     2161 actttttagt aacgctaccg gcataaaatg cccagcccgc cgctgcattc gcgcgagaat
     2221 agtttcatag tcgccatcta accagaggaa atgaacatgg gggctaccct tgcgtaaaat
     2281 atcacgatac tgttttttta atgatgaaca gacaataaat cctgtttcat tctttttata
     2341 aagactgtat gaagcatcat ttaagcgttc cagccaggga agtcgatctt catcagataa
     2401 tggaataccc tgcgacattt tatctatatt tttggctgga tgaagatcgt caccatcaat
     2461 aaatttagca gataataacg cggcaacctt gctaccaatt aatgttttac cactccctga
     2521 aacgcccatc aaaataaagc tttcacccgc catttaaaat ctcctgaatc atcttacctg
     2581 ctgcgatact acgcctggtg gcataataat ttcacgtttt tttatttctg ctgataagaa
     2641 ttacaaggca catcacgtta tgcgtaacat agtaatgtaa caattttctg acgtgatctt
     2701 catcacaaat aatgacaatt aaaaccgctt aaatgcttcc gaggtgtcta cctgaccagt
     2761 gaggaattta tgcaagtgaa aacacagtcc tgcgttgttg cggacaagaa aactgttgcc
     2821 gttaccgagc agacgataga ttggaataat aatggaacat tagtacaaat aacccgaggt
     2881 ggaatttgcg gttccgattt acattattat caggaaggaa aagtaggtaa tttcatgata
     2941 aaggcaccga tggtgttagg tcatgaagtt atcggtaaag ttattcatag cgactcatca
     3001 gaattacatg aagggcaaac ggtagccatt aatccgtcta aaccgtgcgg tcactgcaaa
     3061 tactgcattg aacataacga gaatcagtgt acagatatgc gtttttttgg cagtgccatg
     3121 tatttccctc atgttgatgg tggttttacc cgttataaaa tggtcgaaac gtcgcaatgt
     3181 gtcccttatc cggccaaagc tgacgaaaag gttatggctt ttgccgaacc tttagccgtc
     3241 gcgattcatg ccgcacatca ggccgacgag ttacagggca agcgagtatt tgtttccggt
     3301 gttgggccca ttggctgcct gattgtcagt gctgtgaaaa cactggggtc cgcggaaatt
     3361 gtctgtgccg atgtgagtcc ccgttccctt tcgctggcca aagagatggg tgcggatgtg
     3421 ctcgtaaacc cacaaaacga cgacatggat cactggaaag cggaaaaagg ccatttcgat
     3481 gtcagctttg aagtgtccgg tcatccttca tcagtgaata cctgtctgga ggtcactcgt
     3541 gcacgcggcg taatggtgca ggtagggatg ggaggcgcga tggcagaatt cccaatgatg
     3601 acgttgattg gtaaggagat ttcactcaaa ggctctttcc gttttaccag cgaatttaat
     3661 accgcagtgt catggctggc gaatggcgtt atcaatccac tgcctttact gagtgctgaa
     3721 tatctcttca ctgacctgga agaggcgcta cgtttcgccg gtgataaaac ccaggcagca
     3781 aaagtccagc ttgttttcta agagataaat aaaggaataa tacaatgaac gatctatttt
     3841 cactggcagg aaaaaatatc ttgattaccg gttcagcaca gggcattggc tatttactgg
     3901 caaccggcct gggtaaatat ggcgcacaaa taattattaa tgatattact gccgaacgcg
     3961 cagaacttgc tgtagaaaaa ctccaccagg agggtattca ggccgttgcc gcacctttta
     4021 atgttactca taaacatgaa attgatgccg ccgttgaaca tatcgaaaag gacatcggcc
     4081 ccattgatgt gctggtgaat aacgccggta tccagcgccg tcatcctttt actgagttcc
     4141 ctgaacaaga gtggaatgat gtgatcgcag taaaccagac cgccgtgttc ctggtatcgc
     4201 aagcggtaac tcgtcacatg gttgaacgca aggcaggtaa agttattaat atttgctcga
     4261 tgcaaagcga actgggacgt gacaccatca ccccttatgc cgcatcgaaa ggggcggtaa
     4321 aaatgctcac ccgcggcatg tgcgtcgagc tggcgcgcca caatattcag gtcaacggta
     4381 ttgcgccggg ctatttcaaa acagaaatga ctaaagcact ggttgaggac gaagccttca
     4441 ccgcctggtt gtgcaaacgg acccccgccg cacgctgggg agatccgcag gaactgattg
     4501 gtgctgcggt gttcctttct tcaaaagcct ctgatttcgt aaacggccac ctgttgtttg
     4561 ttgatggcgg catgttagtg gctgtttaac agccaccacc ctacgtgcag cgcgaaagcg
     4621 ctgcttttct ggcactaata agagataaag actatgccat taatcattat tgcggcaggc
     4681 gtcgcgctgc ttcttatcct gatgatcggc tttaaagtta acggctttat tgccctcgtt
     4741 ctggtagctg ccgtcgtcgg atttgccgaa gggatggatg cacaggccgt cctgcactct
     4801 atacaaaatg gtatcggcag cacgctcggc gggctggcaa tgatcctcgg tttcggggcc
     4861 atgttaggca agctgatttc tgatacgggc gcggcacaac gtatcgccac tacgctgatt
     4921 gctacttttg gtaaaaaacg cgtgcaatgg gcgctagtga tcaccggtct ggttgtgggc
     4981 ctcgccatgt tttttgaagt gggttttgtc ctgctgttgc cgttggtatt taccatcgtg
     5041 gcatcatctg gattacccct gttgtatgtt ggcgtaccga tggtagcagc gctctctgta
     5101 acccactgtt ttctgccgcc acatccaggg cctactgcca tcgcgactat ctttgaggct
     5161 aatctcggaa cgactttact gtatggattt atcattacca ttccgacagt tattgtcgca
     5221 ggaccgctgt tttctaaact gctaactcgc tttgagaaag caccaccgga aggcttattt
     5281 aatcctcatc tgtttagcga agaggagatg ccctccttct ggaacagtat ttttgctgca
     5341 gtgatcccgg tcatcctgat ggctatcgcc gccgtttgtg aaattacgtt accgaaaact
     5401 aacaccgtgc gcctcttctt tgaatttgtc ggtaaccctg ccgttgcgct gtttattgcc
     5461 attgttattg cgattttcac actgggccga cgtaatggac gcaccatcga gcaaatcatg
     5521 gatatcattg gggattctat aggcgctatc gcgatgattg tgtttattat cgctggcggc
     5581 ggcgcgttta agcaggtatt agtagatagt ggtgtcgggc agtatatttc acacttaatg
     5641 accggaacta cgctttcgcc gttattgatg tgctggactg tcgcggcgct gttgcgtatc
     5701 gctctgggct ctgccaccgt cgcggccatt accaccgcgg gtgtggtgtt gccgattatc
     5761 aacgttaccc atgccgatcc cgctttaatg gtactggcaa ctggtgcggg cagcgtgatc
     5821 gcgtcacacg taaacgaccc tggcttctgg ctatttaaag ggtattttaa tctgacggtt
     5881 ggtgaaacgt tgcgtacctg gacagtgatg gaaaccctta tttctattat gggtttgctg
     5941 ggcgtgttag ccattaacgc cgtattgcac tgaaagaata aaatattgtg atgtagtttc
     6001 gggggaagat tgaagattcc cccgacgttg ggagaagtta tgcgcaatca cagaatttct
     6061 ttacaggata tcgctacgct ggctggcgta acaaaaatga ccgtgagtcg ttatatccgc
     6121 tcgccgaaaa aggtggcaaa ggaaacaggc gagcgcatcg ccaaaattat ggaagagatc
     6181 aattatatcc ctaatcgcgc tcccggcatg ttgttgaatg cacaaagtta taccctcggt
     6241 atcttgattc cgtcatttca aaatcaactc ttcgctgaca ttctcgcggg aatagaatcc
     6301 gtcacctctg tgcataatta tcagaccctt attgctaatt acaattacga tcgcgattcc
     6361 gaagaagagt cggtgattaa cctgctctct tataacattg acgggattat tctttcggaa
     6421 aaatatcaca ccatcaggac ggtgaaattt ctgcgttcag ccaccattcc ggtcgtggaa
     6481 ttgatggatg tacagggaga acggctggat atggaggtcg gttttgataa tcggcaggct
     6541 gcttttgaca tggtgtgtac catgctggat aagcgagtca ggcgcaaaat tctgtatctc
     6601 ggttccaaag atgacacccg cgatgaacag cgttatcagg ggtattgcga tgcgatgatg
     6661 ctgcataatc tttccccatt acgcatgaat ccacgcgcca tctcatccat tcatttagga
     6721 atgcagttaa tgcgtgatgc ccttagcgca aacccggatt tagacggtgt attttgcacc
     6781 aatgacgata ttgcgatggg tgcgcttctg ctgtgccgcg agcgaaacct cgccgtgcca
     6841 gaacaaattt ccattgcggg ttttcatggg ctggagattg gcaggcaaat gatcccaagc
     6901 ctcgccagcg taattactcc ccgttttgat attggtcgga tggccgccca aatgttgctg
     6961 agcaagatta agaataacga tcacaaccac aacactgttg acctcgggta tcagatttat
     7021 cacggtaaca cgctttaggt tattttctct tcattacaca tattctcttt gccggacgtt
     7081 gcctatactc caggcaggaa actggaggaa atctcatgag cgaacccctg ttaattgccc
     7141 gcacgccgga cacagaactg tttttactgc cgggaatggc taaccgtcac gggctgatta
     7201 ctggcgcaac ggggacgggt aaaactgtca cgttgcaaaa actggcagag tcattgtcgg
     7261 aaatcggtgt tccggtgttt atggccgatg tgaaaggcga tctgaccggc atcgcgcagg
     7321 caggaacggc gtcggaaaaa ctgctcgcaa gactgaaaaa tatcggcgtc aacgactggc
     7381 aaccgcatgc caatccggtg gtggtgtggg atatctttgg cgagaaaggc catccggtgc
     7441 gggcgacggt ttcggatctg gggccgctgt tgctggcacg actgttgaat ctcaacgatg
     7501 tgcaatctgg cgtgctgaat attatcttcc gcattgcgga cgatcaggga ttgttgctgc
     7561 tcgactttaa agatttgcgg gcgattaccc agtacatcgg cgataacgcc aaatccttcc
     7621 agaatcagta cggaaatatc agtagcgcat cggttggtgc catccagcgc ggattactgt
     7681 cgctggaaca gcaaggcgca gaacacttct ttggtgagcc gatgctggat atcaaagact
     7741 ggatgcgcac cgatgccaac ggtaaaggcg ttatcaatat cctcagcgcc gagaaacttt
     7801 atcagatgcc gaaactgtac gccgccagcc tgctgtggat gctttcagag ttatatgaac
     7861 aattgcctga agcgggcgat ctggagaaac caaaactggt gtttttcttc gacgaagcac
     7921 atctgctgtt taacgatgcg ccacaggtac tgctggataa gattgagcag gtgataaggc
     7981 ttattcgctc aaaaggcgtg ggcgtctggt tcgtttcgca aaacccgtca gatatcccgg
     8041 ataacgtgct agggcagctc ggtaatcgcg ttcaacacgc tttgcgtgct tttacgccta
     8101 aagatcagaa agcagtgaag gcagcggcgc aaaccatgcg ggccaatccg gcgtttgata
     8161 ccgaaaaggc aatccaggaa ctggggaccg gcgaggcgtt aatctcgttt ctcgatgcga
     8221 aaggaagtcc ttcagtggtg gaacgggcga tggtgatcgc accttgttcg cgaatggggc
     8281 cggtgacgga agatgagcgt aatggcctga ttaatcactc cccggtgtat ggcaaatatg
     8341 aagatgaggt ggaccgcgag tccgcctatg agatgctgca aaaaggcttt caggccagta
     8401 ccgagcagca aaataatccc cccgtgaaag ggaaagaggt ggcggtggat gacggtattc
     8461 ttggtggatt gaaggatatt ttgtttggca ctaccggacc acgcggcggg aagaaagatg
     8521 gtgtggtgca aacaatggcg aaaagcgccg ctcgccaggt gacgaatcag attgtacgcg
     8581 ggatgttggg gagtttgctg ggggggagaa aaaggtaagt cagacgtgat ctgtgcccct
     8641 caccctaacc ctctccccaa aggggcgagg ggaccgttcg aactcgtatt tgactttgtc
     8701 aacaataata aagggagctt tcgctccctt gttagattac gattttctca ttaacagcca
     8761 caggctgatt aagaagaagc tggcgcttgg caacagtgcg ccgatgatcg gcgggatgcc
     8821 ataaaccaac gtcagcgggc cgaagatctg gtccagtacg tagaagacaa aaccgaagct
     8881 gataccggtg accacacgca cgcccatcgg tacgctacgc agtgggccaa agatgaacga
     8941 cagcgccatc aacatcatca ccgccacgga tagcggctgg aagattttgc tccacatgtt
     9001 gagctgataa cgtccggcat cctgaccgct cgacttcaga tacttcacat agttgtgcaa
     9061 accactgatg gagagtgcat ccgggtccag cgccaccacg cccagtttgt ctggcgtgag
     9121 gttggttttc caggtgccgc tcaccgtctg cgaaccggtg atctgtttcg gattggtcag
     9181 atcagattca tcaacctgcg acagacgcca gactttatgt tccgggtcaa acttcgcggt
     9241 agcggcataa cgtacggatt gcagacgacg attctcgtta aaggcataaa tactgatgcc
     9301 acctaactct tcgtcacctt taacccgctc aatgtagacg aagttgttgc catctttcgc
     9361 ccataagcct tgctgagtgg agagcaacga gccgccgtac atcgcctgcg cacggtagtt
     9421 acgcgccatc tgctcgccct gcgacgcgac ccattcacca atcgccatcg tcagcaagac
     9481 cagcggaatg gcggttttca tcaccgacag cgccacctgc atacgggtaa aaccagaagc
     9541 ctgcatcacc accagttcgc tgcgctgcgc cagcatccca agaccaagca acgccccaag
     9601 cagagccgcc atcgggaaga agatttgcac atctttcggc acgctcagca aggtatacat
     9661 tcctgcgcct aacgcgtcgt aactcccctg cccggctttt ttcagctgat cgacaaactt
     9721 gataatgccc gacagcgaca ccagcatgaa cagcgtcatc atgatggtgg tgaaaatagt
     9781 tttaccgata tagcggtcaa gtacgccaaa aggttgcatc acaccgctcc tttacgcgaa
     9841 aaactggcgc gcaggcggcg gaccggcacg gtgtcccaaa ggttgagaac aatcgccaaa
     9901 gccagataaa tcaggttaac ggtccacatc cacagcgtcg ggtccagctt acctttaccg
     9961 ccgttcgatt tcagggaggt ctggatcagg aagaaaagta gatacagcag catggctggc
    10021 agcatcgaca gtacgcgtcc ctgacgcggg ttaaccacgc tcaacggtac aaccataagt
    10081 gccatcataa acacggtgaa taccaacgtg atacgccagt tcagttctgc gcgggcacga
    10141 tcggtgtcag tgttccacaa tgtgcgcatg tccatctggt cggtatcgtt cgggtcgagc
    10201 accaccgcct ggtgaccaat gatcgcctga taatcctgaa agtccgtaat gcggaaatcg
    10261 cgtaacagtg cagtgccttc gaagcgcgtt ccctggttga gagtgacgac ctgggagccg
    10321 tcgcgcagct gggttaaatg tccggaatcg gccaccacca cagaaggacg tgcattacct
    10381 tttggtcgaa tttgtgcgag gaacacatct ttgaaatcgc tgccgtcaac gctttcgatg
    10441 aacagcaccg agctgccatt agtcgcttgc tggaattgcc cttgcgccag cgccgccatg
    10501 ccagggttcg ctttcgcttc tgctaacact tcatcctgat gacgcgatga ccatggtccc
    10561 gcccacatca cgttaaccgc cgcgacgatt gccgtgaaca ccgcaaggat cattgccgct
    10621 ttcaccagca ccgctttgct cagaccgcag gcatgcatta ccgtaatttc actttcggta
    10681 tacagtttgc ccagcgtcat cagcagcccg aggaacaggc ttaatggcag gataagctgc
    10741 gccatttccg gcacgcccaa cccgagaagg gagagcacca gattcgccgg aatatcgccg
    10801 tcaaccgctg cgccgaggat cctcactaac ttttgacaga agaagatcaa aagcaagatg
    10861 aagagtatcg ccagctggct tttgagcgtc tcccgcacca gatatcttat gattatcact
    10921 ttaaatacgc ccgtaaaaac tcgtcttttg caggatttta gcttgtttca tggcttaaac
    10981 gtcatttatt ctcttgagtc gtcgaaatcg tcgctaagat aattatactc aacggattca
    11041 cctctcagat tttgttctga cgtgccaatg ccgtaataac gttaagatta acacgaagtc
    11101 atcgcaacag cggacatgag ttacgaaagc ttgcaattct atctgtagcc accgccgttg
    11161 tctttaagat tcaggagcgt agtgcatgga gtttagtgta aaaagcggta gcccggagaa
    11221 acagcggagt gcctgcatcg tcgtgggcgt cttcgaacca cgtcgccttt ctccgattgc
    11281 agaacagctc gataaaatca gcgatgggta catcagcgcc ctgctacgtc ggggcgaact
    11341 ggaaggaaaa ccggggcaga cactgttgct gcaccatgtt ccgaatgtac tttccgagcg
    11401 aattctcctt attggttgcg gcaaagaacg tgagctggat gaacgtcagt acaagcaggt
    11461 tattcagaaa accattaata cgctgaatga tactggctca atggaagcgg tctgctttct
    11521 gactgaactg cacgttaaag gccgtaacaa ctactggaaa gtgcgtcagg ctgtcgagac
    11581 tgcaaaagag acgctctaca gtttcgatca gctgaaaacg aacaagagcg aaccgcgtcg
    11641 tccgctgcgt aaaatggtgt tcaacgtgcc gacccgccgt gaactgacca gcggtgagcg
    11701 cgcgatccag cacggtctgg cgattgccgc cgggattaaa gcagcaaaag atctcggcaa
    11761 tatgccgccg aatatctgta acgccgctta cctcgcttca caagcgcgcc agctggctga
    11821 cagctacagc aagaatgtta tcacccgcgt tatcggcgaa cagcagatga aagagctggg
    11881 gatgcactct tatctggcgg tcggtcaggg ttcgcaaaac gaatcgctga tgtcggtgat
    11941 tgagtacaaa ggcaacgcgt cggaagatgc acgcccaatc gtgctggtgg gtaaaggttt
    12001 aaccttcgac tccggcggta tctccatcaa gccttcagaa ggcatggatg agatgaagta
    12061 cgatatgtgc ggtgcggcag cggtttacgg cgtgatgcgg atggtcgcag agctacaact
    12121 gcctattaac gttattggcg tgctggcagg ctgtgaaaac atgcctggcg ggcgtgccta
    12181 tcgtccgggc gatgtgttaa ccaccatgtc cggtcaaacc gttgaagtgc tgaacaccga
    12241 tgccgaaggc cgcctggtac tgtgcgacgt gttaacttac gttgaacgtt ttgagccgga
    12301 agcggtgatt gatgtggcga cgctgaccgg tgcctgcgtg atcgcgctgg gtcatcatat
    12361 tactggtctg atggcgaacc ataatccgct ggcccatgaa ctgattgccg cgtctgaaca
    12421 atccggtgac cgcgcatggc gcttaccgct gggtgacgag tatcaggaac aactggagtc
    12481 caattttgcc gatatggcga acattggcgg tcgtcctggt ggggcgatta ccgcaggttg
    12541 cttcctgtca cgctttaccc gtaagtacaa ctgggcgcac ctggatattg caggaaccgc
    12601 ctggcgttct ggtaaagcaa aaggcgcaac cggtcgtccg gtagcgttgc tggcacagtt
    12661 cctgttaaac cgcgctgggt ttaacggcga agagtaagtt tgcgttttac cctcacccta
    12721 accctctccc taagggagag ggaaccgatc gagcgcaata gaaattcatt gctcatcttt
    12781 cagggtgagg aatgcagcaa tatttaaatc caccacaaga agccccatat atgaaaaacg
    12841 caacgttcta ccttctggac aatgacacca ccgtcgatgg cttaagcgcc gttgagcaac
    12901 tggtgtgtga aattgccgca gaacgttggc gcagcggtaa gcgcgtgctc atcgcctgtg
    12961 aagatgaaaa gcaggcttac cggctggatg aagccctgtg ggcgcgtccg gcagaaagct
    13021 ttgtcccgca taatttagcg ggagaaggac cgcgcggcgg tgcgccggtg gagatcgcct
    13081 ggccgcaaaa acgcagcagc agcccgcgcg atatattgat tagtctgcga acaagctttg
    13141 cagattttgc cactgctttc acagaagtgg tagacttcgt tccttatgaa gattctctga
    13201 aacaactggc gcgcgaacgc tataaagcct accgcgtggc tggtttcaac ctgaatacgg
    13261 caacctggaa ataatggaaa agacatataa cccacaagat atcgaacagc cgctttacga
    13321 gcactgggaa aagcagggct actttaagcc taatggcgat gaaagccagg aaagtttctg
    13381 catcatgatc ccgccgccga acgtcaccgg cagtttgcat atgggtcacg ccttccagca
    13441 aaccatcatg gataccatga tccgctatca gcgcatgcag ggcaaaaaca ccctatggca
    13501 ggtcggtact gaccacgccg ggatcgctac ccagatggtc gttgagcgca agattgccgc
    13561 agaagaaggt aaaacccgtc acgactacgg ccgcgaagct ttcatcgaca aaatctggga
    13621 atggaaagcg gagtccggcg gcaccattac ccgtcagatg cgccgtctcg gcaactctgt
    13681 cgactgggag cgtgaacgct tcacgatgga cgaaggcctg tccaatgcgg tgaaagaagt
    13741 tttcgttcgt ctgtataaag aagacctgat ttaccgtggc aaacgcctgg taaactggga
    13801 tccgaaactg cgcaccgcta tctctgacct ggaagtggaa aaccgcgaat cgaaaggttc
    13861 gatgtggcac atccgctatc cgctggctga cggcgcgaaa accgcagacg gtaaagatta
    13921 tctggtggtt gcgactaccc gtccggaaac cctgttgggc gataccggcg tggccgttaa
    13981 cccggaagat ccgcgttaca aagacctgat tggcaaatat gtcattctac cgctggttaa
    14041 ccgtcgtatt ccgatcgttg gcgacgaaca cgccgacatg gaaaaaggca ccggctgcgt
    14101 gaaaatcact ccggcgcacg actttaacga ctatgaagtg ggtaaacgtc acgcgctgcc
    14161 gatgatcaac atcctgacct ttgacggcga tatccgtgaa agcgcccagg tgttcgatac
    14221 caaaggtaac gaatctgacg tttattccag cgaaatcccg gcagagttcc agaaactcga
    14281 gcgttttgct gcacgtaaag cagtcgttgc tgcggttgac gcactcggcc tgctggaaga
    14341 aattaaaccg cacgacctga ccgttcctta cggcgaccgt ggcggcgtag ttatcgaacc
    14401 aatgctgacc gaccagtggt acgtgcgtgc cgatgtactg gcgaaaccgg cggttgaagc
    14461 ggttgagaac ggcgacattc agttcgtacc gaagcagtac gaaaacatgt acttctcctg
    14521 gatgcgcgat attcaggact ggtgtatctc tcgtcagctg tggtggggtc accgtatccc
    14581 tgcatggtac gacgaagcag gtaacgttta tgttggccgc aacgaagaag aagtgcgcaa
    14641 ggaaaataac ctcggtgccg acgttgccct gcgtcaggac gaagacgttc tcgacacctg
    14701 gttctcttct gcgctgtgga ccttctctac cctaggctgg ccggaaaaca ctgacgcact
    14761 gcgccagttc cacccaacca gcgtgatggt atccggcttc gacatcattt tcttctggat
    14821 tgcccgcatg atcatgatga ccatgcactt catcaaagat gaaaatggca aaccgcaggt
    14881 gccgttccac accgtttaca tgaccggcct gattcgtgac gacgaaggcc agaagatgtc
    14941 caaatccaag ggtaacgtta ttgacccgct ggatatggtt gacggtattt cgctgccgga
    15001 actgctggag aaacgtaccg gcaatatgat gcagccgcag ctggcggaca aaatccgtaa
    15061 gcgcaccgag aagcagttcc cgaacggtat tgagccgcac ggcaccgatg ccctgcgctt
    15121 caccctggcg gcgctggcgt ctaccggtcg tgacatcaac tgggatatga agcgcctgga
    15181 aggttaccgt aacttctgta acaagctgtg gaacgccagc cgctttgtgc tgatgaacac
    15241 ggaaggtcag gattgcggct tcaacggtgg tgaaatgacg ctgtcgctgg cggaccgctg
    15301 gattctggca gagttcaacc agaccatcaa agcgtaccgc gaagcgctgg acagcttccg
    15361 cttcgatatc gctgcaggca ttctgtatga gttcacctgg aaccagttct gtgactggta
    15421 tctggagctg actaagccgg taatgaacgg tggcaccgaa gccgaactgc gcggtactcg
    15481 ccatacgctg gtgaccgtac tggaaggtct gctgcgcctc gcgcatccga tcattccgtt
    15541 catcaccgaa accatctggc agcgtgtgaa agtactttgc ggtatcactg ccgacaccat
    15601 catgctgcag ccgttcccgc agtacgatgc atctcaggtt gatgaagccg cactggccga
    15661 caccgaatgg ctgaaacagg cgatcgttgc ggtacgtaac atccgtgcag aaatgaatat
    15721 cgcgccgggc aaaccgctgg agctgctgct gcgtggttgc agcgcggatg cagaacgtcg
    15781 cgtaaatgaa aaccgtggct tcctgcaaac cctggcgcgt ctggaaagca tcaccgtgct
    15841 gcctgccgat gacaaaggtc cggtttccgt tacgaagatc atcgacggcg cagagctgct
    15901 gatcccgatg gctggcctca tcaacaaaga agatgagctg gcgcgtctgg cgaaagaagt
    15961 ggcgaagatc gaaggcgaaa tcagccgtat cgagaacaag ctggcgaatg aaggctttgt
    16021 cgcccgcgca ccggaagcgg tcatcgcgaa agagcgtgag aagctggaag gctatgcgga
    16081 agcgaaagcg aaactgattg aacagcaggc tgttatcgcc gcgctgtaat caaaacacag
    16141 tgatgaaaac gaaggccgga gcatgctccg gcctttttta tctcttacag aaatggtaaa
    16201 gaaggcataa ttccccgtga aatactggct aagaatcctt tatctggttg ttcttcttta
    16261 ttgattaaag gaaggtcatc caaatcgccc agcaaatacg tattttttgc ctgccagtca
    16321 atcatccata ttttcagcag tggataagcc agaccgcccg taatcccgga tatcaccacc
    16381 aacgcacaca tgcgataaag cataccgtgg tatgttaaag ttgagcgaaa acgaatacgc
    16441 ccatcattca gtgacaggtt gctcataaaa tgatttcgca acgacaccgt taaataactg
    16501 gtgctaacag caatcccaaa ataatagatt aactgcgcga ttatcatttt acgttgcatt
    16561 tccataaatt gctgtaaatt ctcaatatca tcatttgcat acccagaact atcatactca
    16621 tttaatattt gatcgaagat aatataacca gcaacagcga gaaaaggtaa taaagccaaa
    16681 aatgcgagaa tggcatattt tatacaatat gtagtatcta atttcacctt gaaacgatgt
    16741 ataccgaaac tggtattact ccagagaaaa ctcattacca gactatataa agtgccatta
    16801 aaaataccaa tgaaaacaat acaaactatt gtcatcagaa ccacagatat aataacgcta
    16861 cttgaactat tggcatgtag catctttgtc gagataaaga aaacagtccc catcccaagg
    16921 gccattaaaa tcggcaagaa aaaggtcacc caccagaacc ctttcataga gcaattaaaa
    16981 ctaaatctta cgccgttgag actggtcatc aaggcctgat aacgcagtcc ttttgctgcc
    17041 ataaatataa gcaaaaccaa cagtgacaaa gtcaaaacac agccaataag cggcatatct
    17101 gctgacactg tcattaagat tgcgaaatag aaaaaaacaa aaacaagaca actaacaaaa
    17161 acattcccac cggtaatccc ataagaaaat cgttgtccgt taacttccat attagcatag
    17221 agataacgct tacatttcat taatgcccat ggtaaataga tacctagcgt tataatcgtt
    17281 aacaacacat tcaccacaca aataagaaaa tatctctcac ctgtaccatg aaaaacaaac
    17341 gaatgggacg gaacatccat atcattaata acttgagcca taaaagttcc ctgaattaaa
    17401 aaatccattg atataattta tatatacaga cagtaaaatg ttctgcc
//
LOCUS       DAZPDU010000087        17326 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZPDU010000087 DAZPDU010000000
VERSION     DAZPDU010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17326
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.088"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            78..2411
                     /gene="bisC"
                     /locus_tag="ACHURS_002575"
     CDS             78..2411
                     /gene="bisC"
                     /locus_tag="ACHURS_002575"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFR1238271.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVGAAKIRQLAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(2380..2820)
                     /locus_tag="ACHURS_002576"
     CDS             complement(2380..2820)
                     /locus_tag="ACHURS_002576"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFR1238272.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(2817..3380)
                     /gene="tag"
                     /locus_tag="ACHURS_002577"
     CDS             complement(2817..3380)
                     /gene="tag"
                     /locus_tag="ACHURS_002577"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFR1238273.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            3538..4236
                     /locus_tag="ACHURS_002578"
     CDS             3538..4236
                     /locus_tag="ACHURS_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFR1238274.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPNYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTQTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            4465..5673
                     /locus_tag="ACHURS_002579"
     CDS             4465..5673
                     /locus_tag="ACHURS_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1238275.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            5997..7688
                     /gene="eptB"
                     /locus_tag="ACHURS_002580"
     CDS             5997..7688
                     /gene="eptB"
                     /locus_tag="ACHURS_002580"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFR1238276.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKEAAAN"
     gene            7780..7856
                     /locus_tag="ACHURS_002581"
     tRNA            7780..7856
                     /locus_tag="ACHURS_002581"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7814..7816,aa:Pro,seq:cgg)
     gene            7891..8024
                     /locus_tag="ACHURS_002582"
     ncRNA           7891..8024
                     /ncRNA_class="other"
                     /locus_tag="ACHURS_002582"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            8769..10376
                     /gene="dppA"
                     /locus_tag="ACHURS_002583"
     CDS             8769..10376
                     /gene="dppA"
                     /locus_tag="ACHURS_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFR1238277.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQTPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            10684..11703
                     /gene="dppB"
                     /locus_tag="ACHURS_002584"
     CDS             10684..11703
                     /gene="dppB"
                     /locus_tag="ACHURS_002584"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFR1238278.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            11713..12615
                     /gene="dppC"
                     /locus_tag="ACHURS_002585"
     CDS             11713..12615
                     /gene="dppC"
                     /locus_tag="ACHURS_002585"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFR1238279.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYIVIVLF
                     ITIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            12626..13609
                     /gene="dppD"
                     /locus_tag="ACHURS_002586"
     CDS             12626..13609
                     /gene="dppD"
                     /locus_tag="ACHURS_002586"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFR1238280.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            13606..14612
                     /gene="dppF"
                     /locus_tag="ACHURS_002587"
                     /pseudo
     CDS             13606..14612
                     /gene="dppF"
                     /locus_tag="ACHURS_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417997.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
     gene            complement(14642..15913)
                     /locus_tag="ACHURS_002588"
     CDS             complement(14642..15913)
                     /locus_tag="ACHURS_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFR1238281.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            16389..16496
                     /locus_tag="ACHURS_002589"
     CDS             16389..16496
                     /locus_tag="ACHURS_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR1238282.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            16872..16979
                     /locus_tag="ACHURS_002590"
     CDS             16872..16979
                     /locus_tag="ACHURS_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR1238283.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT         4135 a         4312 c         4690 g         4189 t
ORIGIN      
        1 ttattcctgg gaatactcgg aaatttgtaa gtaatattta actgctcaat acatctaacc
       61 tttcaggagt cttcggtttg gccaactcat cctcacgata ttccgttctg actgccgccc
      121 actgggggcc catgctggtt gaaaccgacg gcgaaaccgt gtttagctcg cgtggcgcgt
      181 tagccacagg aatggaaaac tccttgcaga gcgcggttcg cgaccaggtt cacagcaata
      241 cgcgggtacg atttccaatg gtgcgcaaag gctttctcgc gtcaccggaa aacccgcaag
      301 gcattcgtgg gcaggatgaa tttgttcgcg tgagttggga tgaggcgctg gatcttattc
      361 accaacaaca taaacgcatt cgtgaggctt atggtccggc atcgattttt gctggttcct
      421 acggctggcg ttcaaacggc gtgctgcata aggcctcgac attattacaa cgctatatgg
      481 cgctggcagg cggttatacc gggcatctgg gggattattc gaccggtgcg gcacaggcga
      541 tcatgccgta tgtcgtaggt ggcagcgaag tttatcaaca gcaaaccagt tggccgctgg
      601 tgctggaaca tagcgatgtc gtggtgttgt ggagtgctaa cccactcaat acgctgaaaa
      661 ttgcgtggaa tgcatccgat gagcaggggc tttcttactt ttccgcactg cgtgacagtg
      721 ggaaaaagct gatctgcatt gatccaatgc gatcggaaac cgtcgatttc tttggcgata
      781 aaatggagtg ggtggcaccg cacatgggca ccgatgttgc gctgatgctg gggatcgccc
      841 atacgctggt ggaaaatggt tggcacgacg aagcgtttct ggcgcgttgc accacaggtt
      901 atgccgtctt cgcctcttat ttgctgggcg agagtgacgg aatagcgaaa accgccgaat
      961 gggcagcaga gatttgtggt gttggcgcag cgaaaatccg ccagctggcg gctattttcc
     1021 accaaaatac caccatgctg atggcaggct ggggaatgca gcgccaacag tttggtgagc
     1081 aaaaacactg gatgatcgtc acgctggcag caatgttggg gcaaatcggc acacccggcg
     1141 gcggttttgg tctttcttac cattttgcca atggtggtaa ccccacgcgg cgttctgcgg
     1201 tgctctcttc catgcagggc agcttgccgg gtggctgcga tgcggtggat aaaatccctg
     1261 ttgcccgcat tgttgaagca ctggaaaacc ctggtggcgc atatcaacac aacggtatga
     1321 accgacattt cccggatatt cgttttatct ggtgggcggg cggtgccaac tttactcatc
     1381 atcaggatac caatcgtctg atccgtgcct ggcaaaaacc ggagctggtg gtgatctctg
     1441 aatgcttctg gacggcggcg gcgaaacacg cggatatcgt tctgcctgcg actacctctt
     1501 ttgagcgtaa tgatctcacc atgaccggtg attacagtaa tcagcatctg gtgccgatga
     1561 agcaagtggt gccgccacgc tatgaagcgc gtaatgattt tgatgttttt gccgagttga
     1621 gtgaacgttg ggagaagggc ggctatgcgc ggtttacgga aggaaaaagt cagctgcaat
     1681 ggctggaaac gttttataac gttgcccggc agcgcggggc aagccagcag gttgaattgc
     1741 cgccatttgc tgagttctgg caagccaacc agttaattga gatgccggaa aacccggaca
     1801 gcgagcggtt tattcgcttc gctgattttt gccgcgatcc gctggcgcat ccgttaaaaa
     1861 ccgccagcgg caagattgaa atcttctcac agcgtattgc cgattacggt tacccggatt
     1921 gccctgggca tccaatgtgg ctggagccgg acgaatggca gggcaatgcc gaaccagaac
     1981 agttgcaggt actttctgcc catccggcgc accgcctgca cagccagctg aattacagtt
     2041 ctctgcgcga attgtacgcg gtagcaaacc gtgagcctgt caccattcat cctgacgatg
     2101 ctcaggcgcg cggcataacc gaaggggata tggtgcgggt gtggaactcc cgtgggcaga
     2161 tccttgccgg agcggtcatt agcgagggaa ttaaacctgg cgtgatttgc attcacgaag
     2221 gggcatggcc ggatctggat ttaaccgctg acggtatttg taaaaacggc gcagtgaacg
     2281 tgctgaccaa agatctcccc agctcgcggc tggggaatgg ctgtgcgggt aatacggcgc
     2341 tggcatggct ggaaaaatac aacggtccgg aactgacact tacagcgttt gaaccaccgg
     2401 ccagctcata atccaggtgg gtagttgggt ttcatcctgc catgcgcaat cgacaatgtg
     2461 aaaaccctgc gcacggtaaa aatctatcgc cggttgattt ttttgataaa cctccagcat
     2521 cagatgggga tagcgctgct gcacatactg catcagcgcc ttaccaatac cgcgtctgac
     2581 ggccttcggt gcgacaaaca ttgctgccag aaatcggcct tccataatgc tgacaaaacc
     2641 gagaagctta ccgtcctctt cccagaccca gttttgcgcg ttggcaagat aggcatcccg
     2701 caccagcgga atgcagtcac gccagtaatt cgcttttata aagggatgcc cccaggttgt
     2761 actttccagc cacagttcga ggatcgcggg gagttctgaa cgttgcgctt cccgaatcat
     2821 ggtttatttc ccggatagca acagcagcca accacatgat cattcaccag cccacatgcc
     2881 tgcataaagg agtaacagat tgtggtgccg acaaacttaa aaccacgttt tttcagtgct
     2941 ttagataggg cgtcggaggc ggacgtagat gtgggaattt cgctcaacgt tgtggcttgt
     3001 gtcacctgtg gctgatgatt tacaaacgac cagacaaagt cggcaaacgg ttcgccgttc
     3061 tgttccattt gcaggtacgc ccgcgcatta ccaataattg cctgaatttt ccctcgatgg
     3121 cggataatcc cggcgtcctg taccagtctt tcgacatcct cttcctgcat tgctgcgacc
     3181 ttcaccggat cgaactgatg aaagcaggcg cgatagtttt cgcgtttttt gaggacggtg
     3241 atccacgata acccagcctg ctgcccttca aggcagatca tttcgaacag ttttttactg
     3301 tcagtttcag gcacgcccca ttcattatca tggtaggcaa tataaagcgg gtcctgactc
     3361 acccagccgc aacgttccat actttccctc gctatgtacc cgatcgtaaa aatttcagcc
     3421 gcttttcttg acgcatcggt gaaaaagtca atatttaata cagggctact atgcccgata
     3481 tccttcatac aatgacaata atattgccga attcggctct tccctggagt cgtgttaatg
     3541 atcataaaaa aaagcggtgg tcgctggcag ctaagcctgc tggcgagcgt ggtaatcagt
     3601 gccttttttc tcaacacagc ttacgcctgg caacaagaat atatcgttga tacgcaaccc
     3661 ggacattcca cagagcgtta cacctgggat agtgatcatc aacctaatta caacgatatt
     3721 ttgtcgcaac gtattcaaag tagccaaagg gcgctgggac tggaagtcaa tctggcggaa
     3781 gaaactcctg tggatgtgac cagcagtatg agtatgggct ggaattttcc tttatatgaa
     3841 caggttacaa ccggcccggt cgcggcatta cattacgatg gcacaaccac ctcgatgtat
     3901 aacgagtttg gcgacagtac taccacgcag accgatccgt tatggcatgc cagcgtgagt
     3961 accttaggct ggcgtgttga ctcccggctt ggcgatctcc gaccctgggc gcaaatcagc
     4021 tataaccagc aatttggcga gaatatctgg aaggcgcaat caggcctgag ccggatgacg
     4081 gcgacaaacc agaacggcaa ctggctggat gtcaccgtag gcgctgatat gttgctcaat
     4141 caaaatattg ccgcctatgc cgcgctaact caggcagaaa ataccactaa taatagcgac
     4201 tatctgtata cgatgggggt tagcgccaga ttttaacgta acagtcacaa ttgaaaccat
     4261 taaataacaa taattgtggc gatagtgggt gctaacttac caaataataa atttggtgaa
     4321 taattgtcgc gtcattcatt cctgaactaa ggcatttcat tccgttctga tggcatttca
     4381 tgccgttttt ccccaggcat aaagtgcact tcgttatggt tgtcggcaga gatttttcct
     4441 ttttattact gcaggaatac tgccatgaca ccttcaaatt atcagcgtac ccgctggctg
     4501 acactcatcg gtactatcat tacccagttt gcgctggggt cggtttatac ctggagcctg
     4561 tttaatggcg cgctttccgc caagctggat gcgccggtaa gccaggtcgc tttctctttc
     4621 ggcttgttaa gtctggggct ggcaatttcg tcttctgttg cgggcaaatt acaggaacgt
     4681 tttggcgtta aacgcgtcac catggcttcc ggcattttgc tgggattagg cttcttcctg
     4741 accgcgcatt ccaacaacct gatgatgctg tggttaagcg ccggtgtgct ggtcggtctg
     4801 gcggatggcg cgggttacct gctgacgctc tctaactgcg tgaagtggtt cccggagcgt
     4861 aaaggtctga tctccgcgtt cgctatcggt tcttatggtc tgggtagcct gggtttcaaa
     4921 tttatcgaca cgcagctgct cgaaacggtg ggtctggaaa aaacatttgt gatttgggga
     4981 gcgattgcgc tggtgatgat tgttttcggc gcaacgttaa tgaaagacgc accaaaacag
     5041 gaagtgaaaa ccagcaatgg tgtggtggag aaagattaca cgctggcaga gtcgatgcgt
     5101 aaaccgcagt actggatgtt agcggtaatg ttcctgactg cctgcatgag cggcctgtac
     5161 gtgattgggg tagcgaaaga tatcgcccaa agtctggcac accttgatgt ggtttccgca
     5221 gccaatgcag taacggttat ttccatcgct aacctttcag gtcgtctggt gctgggcatt
     5281 ctgtctgaca aaattgcccg tatccgtgtt atcaccattg gtcaggtgat ttcgctggtg
     5341 ggtatggcgg ccctgctgtt tgcaccatta aatgcagtga cgttctttgc agcgattgcc
     5401 tgcgtggcat ttaactttgg cggcactatt accgtctttc catcactggt cagtgagttc
     5461 ttcggcctca ataacctggc gaaaaactac ggtgtgattt acctcggttt cggtatcggt
     5521 agcatttttg gttcgattat cgcctcactg tttggcggtt tctatgtgac cttctacgtg
     5581 attttcgccc tgctgattct gtcattggcg ctttctacga cgattcgtca gccagagcag
     5641 aaaatgttgc gtgaggcgca tggctccctt taatgctcag ccgcgagcta ctgggcttca
     5701 gcttccgtgt gcatcaaacg catcaacgaa atgtataaac aatgactcgt aaaatttggg
     5761 ccagttaact ggcccttttt actgcttttt gtaacgctaa agtctctttt caaacttgca
     5821 tttttgtaaa tttgtgcttc atgcacactc tttccccaca ctttttcgct ttgctgtggt
     5881 ctacttattc gcgcgtgtag attttactta tctgactacc tccgcacttt tcccctgccg
     5941 ggcctgaaaa gccgttaagc agggtgttat cacctgtttg tccagggttt gtttgcatga
     6001 gatacatcaa atcgattaca cagcagaagc tgagcttttt gcttgcaatc tatattggcc
     6061 tttttatgaa tggcgcggtt ttttatcgcc gcttcggcag ctatgcgcac gattttaccg
     6121 tctggaaagg cgtttctgct gttgttgaac tggccgccac cgtactggtg accttctttt
     6181 tactacgtct tctatcgctg tttggccgcc gcagctggcg tattctggcg tcgctggtgg
     6241 tgctcttttc cgcaggtgcc agctattaca tgaccttcct taatgtggtc attggttatg
     6301 gcatcatcgc ttccgtcatg accaccgata tcgacctgtc aaaagaagtt gttggtctga
     6361 actttattct ctggttaatc gccgttagtg cattgcctct tatccttatc tggaataacc
     6421 gctgtcgcta caccttgctc cgacaactgc gaaccccagg gcagcgtatt cgcagcctgg
     6481 cggtcgtcgt actggcgggt attatggttt gggcaccgat tcgtttgctg gatatccagc
     6541 agaagaaagt ggagagggcg accggcgttg atttgccgag ttatggcggt gtcgtagcga
     6601 actcttatct gccatcaaac tggctttctg cgttggggct gtatgcctgg gcgcgggtcg
     6661 atgaatcttc cgataataat tcattgctta atccggcgaa gaagtttaca taccaggctc
     6721 cacaaaacgt tgatgatact tatgtcgtgt ttatcatcgg tgaaaccacg cgttgggacc
     6781 atatgggtat tttcggctat gagcgtaata ccacgccgaa actggcccag gagaaaaatc
     6841 tggcggcgtt ccgtggttac tcctgtgata ccgcaaccaa actctcactg cgttgcatgt
     6901 ttgtacgtca ggggggcgcg gaagataatc cgcagcgcac attaaaagaa cagaacattt
     6961 tcgcggttct gaagcagtta ggattcagtt ctgacctcta cgctatgcag agcgaaatgt
     7021 ggttctacag caacacgatg gcggacaaca ttgcttatcg tgagcagatt ggtgcggagc
     7081 cacgtaatcg tggcaagccg gtagatgata tgttgctggt agacgaaatg cagcaatcgc
     7141 tagggcgcaa cccggatggt aagcatctga tcattctgca taccaaaggt tcgcatttta
     7201 actacaccca gcgttatccg cgtagcttcg cgcagtggaa gccggaatgt attggtgttg
     7261 atagcggctg taccaaagcg cagatgatca actcctatga caactcggtg acctatgtgg
     7321 atcactttat ctccagcgtg attgatcagg ttcgcgataa gaaagcgatt gtgttctacg
     7381 cagctgacca cggtgagtca attaatgaac gcgagcacct gcacggcacg ccgcgtgaac
     7441 tggcaccgcc ggagcagttc cgcgtaccga tgatggtctg gatgtcagat aaatatctgg
     7501 aaaatccggc caatgcgcag gcgtttgcgc agctgaaaaa agaagccgac atgaaagtgc
     7561 cacgccgtca cgtagagctg tacgacacca tcatgggttg tcttggctat acttcaccgg
     7621 atggtggaat taacgaaaac aacaactggt gtcacatccc gcagacaaaa gaggcagcgg
     7681 ctaactaaga ggcctgctga ctttctcgcc gatcaaaagg cattttgcta ttaagggatt
     7741 gacgagggcg tatctgcgca gtaagatgcg ccccgcattc ggtgattggc gcagcttggt
     7801 agcgcacttc gttcgggacg aaggggtcgg aggttcgaat cctctatcac cgaccaaatt
     7861 cgaaaagcct gctcagcgag caggcttttt tgcatttgaa gcccatgagg atgagaacct
     7921 ccgggggcgg ggaggttcga ctcgagcgaa gcgagagaac gttgcgccag caacggcccg
     7981 cagggcgagc cacgaagtgg cgagtaatcc tctatcaccg accaaattcg aaaagcctgc
     8041 tcaacgagca ggcttttttg catttgtagc tcatgaggat gagaacctcc gggggcaggg
     8101 aggttcgact cgacgtaggc ctgataagac gcgccagcgt cgcatcaggc aacggcaccg
     8161 catttattgt cggatgcggc gcgaccgcct tattcgacca acacaccaca cattttcctg
     8221 ccaactttgc gccccatatc tcactatcca ttaacggatt tgtgacagaa accattgtct
     8281 ttttttatat ttgcttaaat ctttttttgc gttacttatg gctggcgctg gcattttccg
     8341 ctgtgcgttt tagcgttcat ggtattaatc gctcagatat ccaccttttc ctcagatttt
     8401 tttcattaaa tgcataaaac ttaatcacca aatgttgcta aatattaagc tgattgttct
     8461 ggttatgttg gagtagcaca aatttctctg ctgcaaatag ctgtttgaag tttttttaat
     8521 ctttgtttgt ggattctcac cgttggtgta aatcccggtt ggctgtattg acgttttcac
     8581 attctgttga cagattgtag gtcacgaggg gcattttatg gaggatccgc actgttacac
     8641 tgatgttaat tagtacggca tccccacctc ataacgttga cccgaccggg caaaaaacaa
     8701 aaaaggtcag gcagcgacaa cccactgcaa agggttaaaa caacaaacat cacaattgga
     8761 gcagaataat gcgtatttcc ttgaaaaagt cagggatgct gaagcttggt ctcagcctgg
     8821 tggctatgac cgtcgcagca agtgttcagg ctaaaactct ggtttattgc tcagaaggat
     8881 ctccggaagg gtttaacccg cagctgttta cctccggcac cacctatgac gcctcttccg
     8941 taccgcttta taaccgcctg gttgaattta aaatcggcac cactgaagtg atcccgggcc
     9001 tcgctgaaaa gtgggaagtc agcgaagacg gtaaaaccta taccttccat ctgcgtaaag
     9061 gtgtgaagtg gcacgacaat aaagaattca aaccgacgcg tgaactgaac gccgatgacg
     9121 tggtgttctc gttcgaccgt cagaaaaacg cgcaaaaccc gtaccataaa gtctctggcg
     9181 gcagctacga atacttcgaa ggcatgggct tgccggagct gatcagtgaa gtgaaaaagg
     9241 tggacgacaa cacagttcag ttcgtactga ctcgcccgga agcgccgttc ctcgctgatc
     9301 tggcaatgga cttcgcctct attctgtcaa aagaatatgc tgatgcgatg atgaaagccg
     9361 gtacaccgga aaaactggac ctcaacccaa tcggaaccgg tccgttccag ttacagcagt
     9421 accaaaaaga ttcccgtatc cgctacaaag cgtttgatgg ctactggggc accaaaccgc
     9481 agatcgatac gctggttttc tctattaccc ctgatgcttc cgtgcgttac gcgaaattgc
     9541 agaagaatga atgccaggtg atgccgtacc cgaacccggc agacatcgct cgcatgaagc
     9601 aggataaatc catcaacctg atggaaatgc cggggctgaa cgtcggctac ctctcctata
     9661 acgtgcagaa aaaaccgctg gatgacgtga aagttcgcca ggcactgacc tacgcggtga
     9721 acaaagacgc gatcatcaaa gcggtttatc agggcgcggg tgtatcagcg aaaaacctga
     9781 tcccgccaac catgtggggc tataacgacg acgttcagga ttacacctac gatcctgaaa
     9841 aagcgaaagc cttgctgaaa gaagcgggtc tggaaaaagg tttctccatc gacctgtggg
     9901 cgatgccggt acaacgtccg tataacccga acgctcgccg catggcggag atgattcagg
     9961 cagactgggc gaaagtgggc gtgcaggcca aaatcgtcac ctacgaatgg ggtgagtacc
    10021 tcaagcgtgc gaaagatggc gaacatcaga cggtaatgat gggctggact ggcgataacg
    10081 gggacccgga taacttcttc gccaccctgt tcagctgcgc cgcctctgaa caaggttcta
    10141 actactcaaa atggtgctac aaaccgtttg aagatctgat tcaaccggcg cgtgctaccg
    10201 acgaccataa caaacgcgtt gaactgtaca agcaagcgca ggtggtgatg cacgatcaga
    10261 ctccggcact gatcatcgct cactccaccg tgtttgaacc ggtacgcaaa gaagtcaaag
    10321 gctatgtggt tgatccatta ggcaaacatc acttcgaaaa cgtctctatc gaataattaa
    10381 aagccataca agactgatgg caaaggcaaa actgcctgat gtgctccgct tatcaggcct
    10441 acgaaaattc tgcaatgtat tgaatttgca cgattttgta ggccggataa ggcgttaacg
    10501 ccgcatccgg cataaacaaa gcgcactttg tcaacaatct gtatacccgg tggcgctgtg
    10561 cctctgcgtg cagcgccatc cggcagcaat acttcattcc ctgtccgata agggcgggaa
    10621 ttgatttgtg agcaatacag acacgcagtt ccaggctgcg ggtcactata gagaatccgg
    10681 gttatgttgc agtttattct ccgacgtttg ggactcgtca tccccacgtt tatcggtatt
    10741 acccttctca catttgcctt tgtccacatg atcccgggcg atccggtgat gatcatggca
    10801 ggcgaacgcg ggatctcccc tgagcgtcac gcgcagctgc tggctgaact cggcttagat
    10861 aaaccgatgt ggcagcagta tctccattac atttggggcg taatgcacgg cgatctaggc
    10921 atttcaatga aaagccgaat tccagtatgg gaagagttcg tgccgcgctt tcaggccacg
    10981 ctggaacttg gcgtctgcgc gatgattttt gctacggcag tcggtattcc ggttggtgtg
    11041 ctggctgcgg ttaaacgcgg ttccattttc gatcacactg cagttggcct ggcgctgacc
    11101 ggctactcga tgccgatctt ctggtggggc atgatgctga tcatgctggt ttcggtgcac
    11161 tggaacctga cgcccgtctc cggtcgcgtg agcgatatgg tgttcctcga tgactccaat
    11221 ccgttaaccg gttttatgct gatcgacacc gccatctggg gtgaagatgg caactttatc
    11281 gacgccgttg cccatatgat cttaccggct attgtgctgg gtactatccc gctggcggtt
    11341 atcgttcgta tgacgcgctc ttcgatgctg gaagtgctgg gtgaagatta catccgcacc
    11401 gcgcgcgcca aagggttgac ccgcatgcgg gtgattatcg tccatgcgct gcgtaacgcg
    11461 atgctgccgg tagtgaccgt tatcggcctg caggtgggaa cattgctggc gggggcgatt
    11521 ctgaccgaaa ccatcttctc gtggcccggt ctgggacgct ggttgattga cgcactgcaa
    11581 cgccgcgact atccggtagt gcagggcggc gtattgctgg tggcgacgat gattatcctc
    11641 gtcaacctgc tggtcgatct gctgtacggc gtggtgaacc cgcgtattcg tcataagaag
    11701 taaggggaca tcatgtcaca ggttactgaa aataaagtga ttagcgcacc ggtgccgatg
    11761 accccgttac aggagttctg gcactatttt aaacgcaaca aaggcgcggt tgtcgggctg
    11821 gtttacatcg tcatcgtgct gtttatcacg atctttgcca actggattgc gccttataac
    11881 ccggcggatc agttccgcga tgcgctgctc gccccgccag cctggcagga aggcggcagc
    11941 atggcgcact tgctgggcac cgatgacgta ggccgtgatg tgctgtcgcg gctgatgtac
    12001 ggcgcgcgct tgtcgctgct ggttggctgt ctggtggtgg tgttatcgct gattatgggc
    12061 gttattctcg gcctgatcgc cggttacttc ggcggcctgg tcgataacat catcatgcgc
    12121 gtggtcgata tcatgctggc gctgccaagt ctgctgttgg cactggtgct ggtggcgatt
    12181 ttcggcccgt cgattggtaa cgccgcgctg gcactgacct tcgttgcctt gccgcactat
    12241 gtgcgcttaa cccgcgccgc cgtgctggtg gaggtgaatc gcgattacgt taccgcgtct
    12301 cgcgtggcag gtgccggggc gatgcgtcag atgtttatta acatcttccc gaactgcctg
    12361 gcgccgctga ttgttcaggc gtcgctcggt ttctctaacg ccattctcga tatggccgcc
    12421 cttggcttcc tcggcatggg agcgcagccg ccaacaccag agtggggcac catgctctcc
    12481 gacgtgttgc agttcgcgca aagcgcctgg tgggtcgtga ccttcccggg tctggcgatc
    12541 ctgctgacgg tgctggcatt taacctgatg ggtgacggtc tgcgtgacgc gctcgatccc
    12601 aaactgaagc agtaagaggt tcgagatggc gttattaaat gtagataaat tatcggtgca
    12661 tttcggcgac gaaagcgcac cgttccgcgc cgtagaccgc atcagctaca gcgtaaaaca
    12721 gggcgaagtg gtcgggattg tgggtgagtc tggctccggt aagtcggtca gttcactggc
    12781 gattatgggg ctgattgatt atccgggccg cgtaatggcg gaaaagctgg tgtttaacgg
    12841 tcaggatttg cagcgtatat cagaaaaaga gcgccgcaac ctggtgggtg ccgaagtggc
    12901 gatgatcttc caggacccga tgaccagcct taacccgtgc tacaccgtgg gtttccagat
    12961 catggaagcg attaaggtgc atcagggcgg taataagagc actcgccgtc agcgggcgat
    13021 tgacctgctg aatcaggtcg gtattcccga tccggcatcg cgtctggatg tttacccgca
    13081 tcagctttcc ggcggcatga gccagcgcgt gatgatcgcc atggcgattg cctgtcggcc
    13141 aaaactgctg attgccgatg agccgaccac tgcgctggac gtgaccattc aggcgcaaat
    13201 catcgaactg ttgctggagc tacagcagaa agagaacatg gcgctggtgt taattaccca
    13261 tgatctggcg ctggtggcgg aagctgcgca taaaatcatt gtgatgtatg caggccaggt
    13321 ggtggaaacg ggcgatgcgc acgccatctt ccatgcgccg cgtcacccgt atactcaggc
    13381 attgctgcgt gcgctgccag aatttgctca ggacaaagaa cgtctggcgt cgttgccagg
    13441 tgtcgttccc ggcaagtacg accgcccgaa cggctgcctg cttaacccgc gctgccccta
    13501 tgccactgac agatgtcgcg ctgaagaacc ggcgctgaat atgctcgctg acgggcgcca
    13561 gtccaaatgc cattacccac ttgatgatgc cgggaggccg acactatgag tacgcaagag
    13621 gccaccctgc aacaaccgct gttgcaggct atcgacctga aaaaacatta tccggtgaag
    13681 aaaggcatgt tcgcgccgga acgtctggtt aaagcgctgg atggcgtttc gtttaacctt
    13741 gaacgtggca aaacgctggc agtagtgggc gaatctggct gcggtaaatc gaccctcggt
    13801 cggttgctga cgatgattga aacgcccacc ggtggcgagc tgtattacca ggggcaggat
    13861 ctgcttaagc acgatccgca ggcgcagaag ctgcgtcggc agaaaatcca gatcgtcttc
    13921 cagaaccctt acggttcgct gaatccgcgt aaaaaagtcg ggcaaattct tgaagagccg
    13981 ctgctgatca acaccagctt aagcaaagaa cagcgtcggg aaaaagccct gtcgatgatg
    14041 gcgaaagtcg gcctgaaaac cgagcactat gaccgctatc cgcatatgtt ctccggcggt
    14101 cagcgtcagc gtatcgccat cgcccgtggt ctgatgctcg acccggatgt ggtgattgcc
    14161 gatgaaccgg tttccgcgct ggatgtttca gtgcgcgcgc aggtgctgaa tctgatgatg
    14221 gatttgcagc aggagttggg gctgtcttat gtctttatct cccacgacct gtcggtggtg
    14281 gagcacattg ctgatgaagt gatggtgatg tacctgggcc gctgcgtgga gaagggaacg
    14341 aaagaccaaa tcttcaataa cccgcgccat ccgtacactc aggcgctact ttccgcgacg
    14401 ccgcgcctga acccccggac gatcgccgcg agcgcatcaa gctcagcggt gaactaccaa
    14461 gcccactgaa tccaccgccg ggttgcgcct tcaacgcccg ctgtcgtcgg cgcttcggcc
    14521 cctgcaccca gttgcagccg cagctaaaag actacggcgg tcaactggta gcttgttttg
    14581 ctgttgatca ggatgaaaat ccgcagcgtt aactcacaaa accggggcca tgcgctccgg
    14641 tttactcaat cagcttcaac agtggcgaca agcatagcaa aatgccgtac agcagaatca
    14701 gccaggcttt aaatccgcga agtttttcca gttgtgcgac tttatagatc aggaaaaacg
    14761 gaatgaggca cgacacaata ccatataacg ggcttcccaa ctgaaagaac accagcaccg
    14821 atacacgaaa cgaaacccaa atcgtcaacg taatgacgat aaaagcgcag atcgccagag
    14881 tcagcacgcg tgagttaatt ttcttggtat caataattcg gcttaacaga ttgagaataa
    14941 tgcctttaat ggcctcgtgg aaaccgagat aaatgccaaa gaatgcggtc agtacggcaa
    15001 agatattaag caccgtagag gtgatatgaa tgatatgccc agggatcacc tgcgcggcca
    15061 gcgccagtgc tgagatattt tgttcaaagg cagaaacggc ttcttcgtgg ctaattgaga
    15121 aggtaaacga aaaggcaaaa aacaggatca ccgcgatgag cgtgatataa ctaattcggt
    15181 gggtacgcag cgcgagccgg gttgccagta ctttatccgc ttcccgttta cgataggcaa
    15241 tattcattgg gttaagtacc tgaataaata ctgcagaaaa gaagcaaaat ggaatggtaa
    15301 gcaagacatc gcggaaaaag acggaggctt gcgggaaggc ggttatattg gcgaaattcc
    15361 agtgcgggat catcgcaaaa ccgaacacga caataatccc tactttgacc accaccattg
    15421 gcccggaaat cttaaacagt aatcgttcac caccagacgc aatcgccacc agtacggcga
    15481 aaatagcgac tttataaagt agagattgtg aaagatcggc atcggttaaa ccgaaggttt
    15541 tcaggtacga ggcgctgtcg aaaaccacgg agagagagta gataaaaatc ccgtggataa
    15601 tcatcaaaaa gtagataacc ccgaggaaaa ttccccagtt cttccccagg taatgactga
    15661 taatatcggt gtagtcatta caggaatcgc tttcagaaag ggtttttaaa taaatgtcct
    15721 gcactaccca ggtggcagga taagcaatga tcgctgcggt aataaatacc caaattccct
    15781 tcaagccaat ttgtactggc atcagcacgg ttccggcacc aatcgccatg cctatgcata
    15841 ataaaaccca gccgaagtcg tagcgtgtaa acggcaattt ctgattgtgt ttcgatagtg
    15901 tgttgtgctg cataaagtcc tgtccagaaa gaagtcggcc tcgcaaaaat gaataaaccc
    15961 agtttctctt gcgcaacagg tggagaagtc gaatacgcag tatgtaaaaa atgaggcaac
    16021 agattcactg gggaggtcgc gaggaggcgc aattctacaa aattgcgcat ttcactacca
    16081 gtctggagtc agcccggata tgaactgcaa ctattgcaaa taaagaaatt aagctggaag
    16141 gcatcgcgca atgaggaaga ttgccgggtg ctgacagtaa aaagcaatcg taaaatcagc
    16201 ggcaagccga aagggttccg gtgaggcgca atgttgcggg ggctttatcc ctggtggcat
    16261 tggttgctgg agagagaaaa cccccgcacg ttgcaggtat gtacctgaca acataacggg
    16321 ggctaatctt gaacctagac cactcaagaa tagccgcgaa acgttgtcat tacaacacag
    16381 gcggctatat gacgctcgca gagctgggca tggccttctg gcatgattta gcggctccgg
    16441 tcattgctgg cattcttgcc agtatgatcg tgaactggct gaacaagcgg aagtagcgtg
    16501 tcatgtgggc gtcaggctgc cgtaatggca atttgcgccc ggaccaggcc gcaggagtga
    16561 aactctgcgg cctttttcgt aagaaaaatg caacggttgc aagcggataa tattctgcgg
    16621 aatgcgcagc acagagtgaa gatgagacgg attccggaac agaaaaacag tcgtagaatc
    16681 agcgtcaagc cggaaggatt ccggtgaggc gcaatgttgc gggggcttaa tccctggtgg
    16741 cattggttgc tggagagaga aaacccccgc acgttgcagg tatgcacctg acaacaccac
    16801 gggggccatt cttgcatcca aacaatacaa gaatagccgc gaaacgttgt cattacaata
    16861 caggcggcta tatgacgctc gcagaactgg gcatggcctt ctggcatgat ttagcggctc
    16921 cggtcattgc tggtattctt gccagtatga tcgtgaactg gctgaacaag cggaagtaac
    16981 gtgccatgcg ggcgtcaggc tgccgtaatg gcaatttgcg cccggatcag gccgcagggg
    17041 tgaaactctg cggccttttt cgtaagaaaa atgcaacggt tgcaagcgga taatattctg
    17101 cggaatgcgc agcacagagt gaagacgtga cggattccgg aacagaaaaa cagtcgtaga
    17161 atcagcgtca agccggaagg gttccggtga ggcgcaatgt tgcgggggct ttatccctgg
    17221 tggcattggt tgctggagag agaaaacccc cgcacgttgc aggtatgcac ctgacaacat
    17281 cacgggggcc attcttgaac ctgaacaact caagaatagc cgcgaa
//
LOCUS       DAZPDU010000088        17303 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZPDU010000088 DAZPDU010000000
VERSION     DAZPDU010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17303
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.089"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            92..1132
                     /locus_tag="ACHURS_002591"
     CDS             92..1132
                     /locus_tag="ACHURS_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFR1238284.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLHTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(1164..2357)
                     /gene="lplT"
                     /locus_tag="ACHURS_002592"
     CDS             complement(1164..2357)
                     /gene="lplT"
                     /locus_tag="ACHURS_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFR1238285.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(2350..4509)
                     /gene="aas"
                     /locus_tag="ACHURS_002593"
     CDS             complement(2350..4509)
                     /gene="aas"
                     /locus_tag="ACHURS_002593"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFR1238286.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            5095..6126
                     /gene="galR"
                     /locus_tag="ACHURS_002594"
     CDS             5095..6126
                     /gene="galR"
                     /locus_tag="ACHURS_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFR1238287.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(6133..7395)
                     /gene="lysA"
                     /locus_tag="ACHURS_002595"
     CDS             complement(6133..7395)
                     /gene="lysA"
                     /locus_tag="ACHURS_002595"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFR1238288.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            7517..8452
                     /gene="lysR"
                     /locus_tag="ACHURS_002596"
     CDS             7517..8452
                     /gene="lysR"
                     /locus_tag="ACHURS_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFR1238289.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(8439..9131)
                     /gene="ygeA"
                     /locus_tag="ACHURS_002597"
     CDS             complement(8439..9131)
                     /gene="ygeA"
                     /locus_tag="ACHURS_002597"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFR1238290.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(9262..10680)
                     /gene="araE"
                     /locus_tag="ACHURS_002598"
     CDS             complement(9262..10680)
                     /gene="araE"
                     /locus_tag="ACHURS_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFR1238291.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(10995..11756)
                     /gene="kduD"
                     /locus_tag="ACHURS_002599"
     CDS             complement(10995..11756)
                     /gene="kduD"
                     /locus_tag="ACHURS_002599"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFR1238292.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQRNGGKIINITSMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(11786..12622)
                     /gene="kduI"
                     /locus_tag="ACHURS_002600"
     CDS             complement(11786..12622)
                     /gene="kduI"
                     /locus_tag="ACHURS_002600"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFR1238293.1"
                     /translation="MDVRQGIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(12909..14090)
                     /locus_tag="ACHURS_002601"
     CDS             complement(12909..14090)
                     /locus_tag="ACHURS_002601"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFR1238294.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            14345..15574
                     /locus_tag="ACHURS_002602"
     CDS             14345..15574
                     /locus_tag="ACHURS_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR1238295.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSACEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            16034..16666
                     /locus_tag="ACHURS_002603"
     CDS             16034..16666
                     /locus_tag="ACHURS_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1238296.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNTAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
     gene            17000..>17303
                     /locus_tag="ACHURS_002604"
     CDS             17000..>17303
                     /locus_tag="ACHURS_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1238297.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVTQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPREDS"
BASE COUNT         4584 a         4512 c         3983 g         4224 t
ORIGIN      
        1 ccggctggcc ttaagatttt tctctttccc ttttcccttc cctctgccat ttttatattc
       61 cttatgtcgt gattataaaa aggaaacggc tatgcaatat caccgtatac cccacagttc
      121 gctggaagtc agcacgctgg ggcttggcac gatgacgttt ggtgaacaga acagcgaagc
      181 cgacgcccac gcacaactcg actatgccgt cgctcagggc attaacctta tcgacgttgc
      241 cgaaatgtac ccagtacctc cgcgccccga aacgcaaggg ttaaccgaaa cctacgtcgg
      301 caactggctg gcgaaacatg gcagccgcga aaagttaatt atcgcctcca aagtgagcgg
      361 accgtcgcgc aataatgaca agggcatccg cccggatcag gcgctggatc ggaagaatat
      421 ccgcgaagcg ctgcatgaca gcctcaagcg cctacacact gattacctcg atctttatca
      481 ggtgcactgg ccgcagcgcc cgaccaactg cttcggcaaa ctcggttata gctggacgga
      541 ttctgcgcct gcggtttcgc tgctggatac gctggacgca ctggcagagt accaacgcgc
      601 gggaaaaatt cgttatatcg gcgtgtcgaa cgaaactgca tttggcgtaa tgcgctacct
      661 gcatctggcg gacaaacacg atctgccgcg tattgtcacc attcagaacc cttacagtct
      721 gttaaaccgc agttttgaag taggtctggc agaagtaagc cagtatgaag gggtcgaact
      781 gctggcctat tcgtgcctgg gtttcggcac gctgaccggg aaatatctca atggtgcaaa
      841 acccgctggc gcacgtaata cgctctttag tcggttcacc cgctatagcg gtgagcaaac
      901 gcaaaaagcc gtcgcggcgt atgttgatat cgccagacgt catggcctag accctgctca
      961 gatggcgctc gcgtttgtac gccgtcaacc gtttgttgcc agcactctgc tgggcgcaac
     1021 cacgatggat cagctgaaaa ctaacatcga aagtttgcat ctggagttaa gcgaagacgt
     1081 attagctgaa attgaagcgg tgcatcaggt ttatacttat ccggcaccat aagattaacg
     1141 ccggttaaaa ccggcgttaa atattaatga cggcgctgcc agatccacag cgccgttatt
     1201 gccagcgcaa acagcgcacc gaagccaatg ccaatgggca cgaccgggat gcctaccatt
     1261 accgccagcg agtaaatgcc cagcatcaac aacatggcgc tgttttcgcc aaggttttgt
     1321 actgcaatcg cattccccgc cccgacgctt tttttacccc gctcctgtag caacgcattg
     1381 agcggaacga caaaaaagcc ccccatcacg ccaatcagca tcaacaaggc ataggctggc
     1441 agcagctcgt gttgcaggga aaaaatcagt accaccacgc caatcaaaat cccggctggc
     1501 atacagcgtg acacggtttc cagcgtaact aacttcgctg ccgcacctgc gccaaccacg
     1561 ataccaatcg ctaccatcgc gttgagatag gtgggcgtag cgttatcggt aatgcccagc
     1621 gccaccggta cccacagcac caacaggaaa cgcagcgtga cacccgctcc ccagaataaa
     1681 ctggtgccca ccagcgaaaa acgcgtttca ccattgcgcc atagcgaggt gcaggcattc
     1741 aggaaactgc gggtcatgtt gatgagattc caggactgcc ccggacgcgc cgccgccagt
     1801 ttgggaatgt agatattggc aacgaccgca ccaccgtagg ccagtgcgca tgcggccagg
     1861 gcgacgagga catgccagtc agccagcaca ccaccggcta cggaaccgag caaaatcgcc
     1921 gctatggtag aagcttccat taaaccgtta gctttcacta acttactacc cgtggttaat
     1981 tcgccgagaa taccgtattt cgccggtgaa taggctgcag caccaacacc caccagcgta
     2041 tagccgagaa acggattgat accaaagcag atactggctg cgcccagcag cttcaggccg
     2101 ttggcaaaca tcatcacccg gcctttggcg aagctatccg ccacctgccc gacaaacggc
     2161 gcaaaaagaa tgtaagcacc tacaaacacc atttgcagga tgggctggct ccactccgga
     2221 tagaactgcg ctttcagtaa cgccagagtg gcaaacagta gggcattatc gccaaacgca
     2281 gagagaaact gcgccacgat aaccgctttc atccccttcg accacaacga agtgttagtg
     2341 tgcactgact cactcatcgt gttgttccgc ttcgtctacc cagcttttca acgtgacaaa
     2401 gtcaggtttg ccgctgccaa gtaatggcat ctgtttcaga tagcgaatat cgcgcggtac
     2461 agcaagctcc ggcacgccgt gctcgcgggc atactgttgc aacttatcgc gcgtcagttc
     2521 gttatctgtg gtgaaaagca ccagtgcctc gcctttgctg gcatcgctct taatcgcagt
     2581 ggcatggact ttatctggcg aaacaccaag tgccagttgt tccaccattt ccagcgacac
     2641 catttcgcct gcaattttgg caaagcgttt tgcgcggccc tgaatctgca caaagccctg
     2701 ctcgtcaaaa cgcacaatat cgccagtgtc ataccagccg cgctccattt cgccgcgaac
     2761 attctcggcg gtgggcactt ccagtacacc tggcttctcc acccgcagat agccgttcat
     2821 tatgttcggc cctttcagtt gcaggcgtcc gccctcttcg ataccaggga ccgacaacag
     2881 gcgcgcatcc attcctggta gaatacgccc taccgtaccg ggtttcgccg ccatcggtac
     2941 gttgatagaa acgacaggcg cgcattcggt cacgccgtag ccttcaagga tgcgcaggcc
     3001 aaatttatcc tgccaaagct gtttggtact ttcttgtaat ttttctgcgc ctgccaccac
     3061 atagcgtaga cgatagaagt catacgggtt ggcgaaacgc gcgtagtgac cgaggaaagt
     3121 cgaggtgccg aacaacacgg tgcaactgcg gtcatacacc aactccggca caatacggta
     3181 atgcagcggg cttggataaa gaaacacttc tgcacctgtg agcagtggcg taaacaggcc
     3241 taccgtcagc ccaaaggagt gaaacagcgg taacgccgac ataaagcgat cgttggtggt
     3301 gaagtcggca atcgttttaa tctgctcgac gttcgccaga atgcttttat ggctatggac
     3361 gacgcctttc ggatggcctt cagaaccgga ggtaaagagg atcagcgcct cttcttccgg
     3421 ctgctgtttt acctgtgcca gacgtggcat cagcagatgc gcgaagatcc atactttgtc
     3481 ggcagtggtg acatctgctt ttaaatcttc cagatagacc cagcgcacct gagtaagttg
     3541 ctccggcaga tgccagagtt tgcctttatc gagaaactgg cgggaagtga agatggtttt
     3601 gatttcagcc gccgtaatag cactggtcag cccttttacc ccggcagtgt agttcatcat
     3661 tgcgggaatg cggcgacggg cgatggcccc aaaaatcact gccgcactga tgcctgcatt
     3721 gggcagcatt aagccgatgc gttcgccttc aacactgtat ttttcaagga tgcgtccaac
     3781 aaacagcgtt ttcgtaagca atttgcgata ggagtctggg gtaaagttga cgtcttcgac
     3841 acatttcttc ccggctccga agcggtacat tgcactcagt aaagattcgt acagcgtttc
     3901 acgcgggcgc accgccattc gcgcttccat cattatttga tgcagcattt cgccagcgat
     3961 tttgcgacgg tcacgggcac gcggcgcatc cggcatctcc acctgcgttg gtggcaaaat
     4021 atgcagagta atttgcggga acaagcgacg tttaaccaga cctttcaggc ggctgaagtg
     4081 cgtaagttcc gccccttcaa tacgcacagg aataaccgtt gcaccagact tcgccgcgac
     4141 aaaacccgcg ccatcgtaga ttttcatcag cgagcctgtg gtggtgatgc gtccttcagg
     4201 gaaaatcacc actggtcggc cctgttccac cagacgtacc agatgtttaa tcgccatagg
     4261 ttgcgtcggg tcgagaggaa caaagtcgat aaatgatttc agccaacgca tataccactg
     4321 ttggcttatt gaggtgtaaa cggcaaacac tggacgcaca ggtaaaaaca gtccaagcaa
     4381 aatgccatca ataaaagaga cgtgattagg cgtaattaga acgcgctcgc ccttcagtgc
     4441 ctgggtgtca cccgtaacgc gaacgcgata caaaacacgg cacaaatttc gaaaaaagct
     4501 aaaaagcatt ccaactccct ttgctctgat tcagtaaaag cgaatggagg gagattacac
     4561 gagataaaga acgcgagcga cagtaaatta ggtgcgaaaa aaaacctgcg catccgcgca
     4621 ggttggtgca agagacaggg tacgaagagc gtaccgaata atctcaccaa tcaatacctc
     4681 tgggatcttg attgtggtct gcacgacgac tcttcgccag cgagaaaacg caaaggaatg
     4741 aagggaaatg caacgaggtg tgtaaattgt cggttactgt tacagattga tgaccggcaa
     4801 aaaaaaacct gcgcatctgc gcaggctggt gtaattcatg tgctcaaccc gaagttgact
     4861 tcacctatca atacctctgg gatcaccact ttagcaacct gaagccaaac gccaccagca
     4921 gtcaatcgca acagcatttc gcaaagtgta accaaaggtt tgcaatctct ttttttgtct
     4981 tacttatttg cgtttggctc acattcccac gatgaaaaca cgccgcccct tgaaccaacg
     5041 ggcgttttcc gtaacactga aacaatgtaa gcgtttaccc actaaggtat tttcatggcg
     5101 accataaagg atgtagcccg actggcaggc gtttcagtcg ccaccgtttc ccgcgtcatt
     5161 aataattcac ccaaagccag cgaagcgtcc cggctggctg tgcatagtgc aatggagtct
     5221 cttagctatc acccgaacgc caacgcccgt gcgctggcgc agcagaccac tgaaacggtc
     5281 ggtctggtcg ttggtgatgt ttccgatccg tttttcggtg caatggtgaa agcggtcgaa
     5341 caggtggctt atcacaccgg taatttttta ttgattggca atggttacca caacgaacaa
     5401 aaagagcgtc aggccattga gcaactgatc cgccatcgct gtgctgcgtt ggtcgtccat
     5461 gccaaaatga tcccggatgc cgatttagcc tcattaatga aacaaatgcc cggtatggtg
     5521 ctgatcaacc gtatcctgcc tggcttcgaa aaccgttgta ttgctctgga cgatcgttac
     5581 ggtgcctggc tggctacgcg tcatttaatt caacaaggtc atacccgcat tggttatctg
     5641 tgctctaacc actctatttc tgacgccgaa gatcgtctgc aagggtatta cgatgccctt
     5701 gctgaaagtg gtattgcggc caatgaccgg ctggtgacat ttggtgaacc agacgaaagc
     5761 ggcggcgaac aggcaatgac cgagcttttg ggacgaggaa gaaatttcac tgcggtagcc
     5821 tgttataacg attcaatggc ggcgggtgcg atgggcgttc tcaatgataa tggtattgat
     5881 gtaccgggtg agatttcgtt aattggcttt gatgatgtgc tggtgtcacg ctatgtgcgt
     5941 ccgcgcctga ccaccgtgcg ttacccaatc gtgacaatgg cgacccaggc tgccgaactg
     6001 gctttggcgc tggcggataa tcgccctctc ccggaaatca ctaatgtctt tagtccgacg
     6061 ctggtacgtc gtcattcagt gtcaactccg tcgctggagg caagtcatca tgcaaccagc
     6121 gactaaccgc agttaaagca attccagcgc cagtaattct tcgatggtct ggcgacggcg
     6181 aatcaaccgc gcctgaccat tatcaaacag aacttctggt aacagcggac ggctgttgta
     6241 gttggatgac attgatgcgc catatgcccc tgtatcatgc agtaccagat aatcacccgc
     6301 cttcacttcc ggcaaggcgc gggtttcaac atttccccct tcctgctggg taaagacatc
     6361 gcccgattca cataatggcc cggcgacgac ggtttccacc gttggcgcgt gttccagaga
     6421 acgaccatca gctgccaggg cactgatatg gtggtaacta ccgtacattg ccgggcgcat
     6481 cagatcgttg aacccggcat caaccagcac aaagtggcgg ctacccattt gtttgacgct
     6541 ccgcacctga gtaattaaca cgccagactg cgctaccaga aagcgacccg gttcaatttc
     6601 cagtttcaca gggtggccca aatggcgagc gatttgctca cgcgcggcat tccacagacc
     6661 ataataatgt tcggtatcaa ccgcctcttc accctgttga taaggaatag aaagcccacc
     6721 gcccgcagaa atagcctgta aatcctgacc gaattcgatg acctgacgta ccatagcacc
     6781 acacacctgt tccagatggg cataatcaac gccagaacca atgtgcatgt gaatgccgac
     6841 cagctgcaga tgatgacgtt gtatcacgtc cagtgcggcg ggcagatcgg tgtaccagat
     6901 accgtgcttg ctgttttcgc caccggtatt ggttttttgg ctatgtccgt gaccaaaccc
     6961 cgggttaacg cgcagccata cccgatgccc tggcgaaacc tggcccagtt ggtcgagcat
     7021 atcaacagaa cccgcattca ccggaatttg caattcactg acgcgttcaa gcgtcgcctg
     7081 atcgataaca tctgccgtaa aaacaatatc atcggggtgc gtttgcggat tgtaacccgc
     7141 cgccaacgca cgctctattt cgcctaacga gacggaatcc actttcacgc cctgctcacg
     7201 cattaatcgc aaaatatgaa tattggaaca ggctttctgt gcaaagcgca ccacatcaaa
     7261 ctgtttcagc gctgcaatct gccgacgaat aatttgcgca tcgtagaccc acaccgggca
     7321 gccaaattca gcgggcaaac gcagcagatt ttcggcggtg agatcggtat cggtgctgaa
     7381 cagtgaatgt ggcataacaa actccagatg agtgcttttt tatgattacg ccacatcata
     7441 aaaagaataa aaaatatcga tttatgtcga gtctatgcaa aaatgatata gattaccgga
     7501 ttgcgagaga gcgctaatgg ccgccgttaa cttacgtcat attgaaattt ttcatgcggt
     7561 aatgaccgcc ggaagcctga ctgaggcagc acacctgcta cacacctcac agccaaccgt
     7621 cagccgcgaa ctggcgcgct ttgagaaggt gatcgggcta aaattgtttg agcgcgtacg
     7681 tggacgatta catcctaccg tgcaaggact gcgtctgttt gaagaagtgc aacgatcctg
     7741 gtacggactg gatcgcattg tcagcgccgc agaaagtctg cgcgagtttc gccagggaga
     7801 actgtctatt gcctgcctgc cggtcttttc gcaatctttt ttaccgcagc tcctgcaacc
     7861 ctttctggca cgttatcccg atgtcagctt aaatatcgtg ccccaggaat caccgctact
     7921 tgaagagtgg ctctcggccc agcgtcatga tttaggactc actgagacac tccatacgcc
     7981 tgcgggaaca gaacgtaccg aattactctc tttagatgaa gtgtgtgtgt tacctccggg
     8041 tcatccgctg gcggtaaaaa aggtattaac gccggatgat tttcagggtg agaactacat
     8101 cagcctttcc cgtactgaca gctatcgcca gttgctggat cagctattta ctgaacatca
     8161 ggttaaacga cgcatgatcg tagaaaccca cagcgccgcg tcagtctgcg caatggtacg
     8221 ggcgggggta ggtgtttcgg tggttaaccc actcaccgca ctggattatg cggcaagcgg
     8281 tttagtggta cggcggttca gtattgcggt tccgttcacc gtcagcctga tccgccccct
     8341 gcaccgcccg tcatcagcgc tggttcaggc gtttagtgag catttacaag cggggttacc
     8401 gaaactggtc acttctcttg acgcgatttt gtcgtcagct acgacagcat aaaagcgaca
     8461 gcatcctcgg catggatcgc cgcagtatca aacacaggca aaacactgcg ctcttctgga
     8521 accaataaac caatttctgt gcagccaaaa atgacaccct gcgccccctg ttctgcaagg
     8581 cgagcaatca cttgcgcata ataagcgcgt gacgcttcgg taaattgccc cagacacagt
     8641 tcttcaaaaa taatctgatt aatcttcgcc cgttcatccg cttccggaat aagacaattg
     8701 atggaaaatt gttccgtcag ccgcccgcga taaaaatcct gttccatggt gtaacgcgta
     8761 cccagcagcg ccacacgagt cattccggcc ccggtaattg cacgtccggt ggcatccgca
     8821 atgtgtaaga aaggcagagt gcaacgtgac tcaatggcat ccgccacttt atgcatcgta
     8881 ttggtacata acacaatacc ttctgcgccc gcccgctgta agccaagcgc cgcctcagcc
     8941 agaatgtccc cggttttatc ccattccccg cgacgctggc actcttctat ttcatgaaaa
     9001 tcgacgctat gtagcagcac ttgcgcagag tgaagcccac caagccgctg tttaatgcct
     9061 tcatttatca gacgatagta aggaatggtg gattcccagc tcattcctcc cagcaaacca
     9121 attgttttca ttcctctctc tcctgatgtg tgttaccgca acaaggctga ataaggaacg
     9181 agacaaacgc ctcaacggcc aggtgcccaa tctctattaa cgaaaaaagg gccggatgta
     9241 cagcacatcc ggcccgtgaa atcagacgcc gatatttctc aacttctcgc ctgccatcag
     9301 tttgcgttcg atatgttcca gcgtgacatt tttggtttcc ggaatgagcc agaaagtgat
     9361 gcccacaaac gcaatgttca gcgcagtgta gagccagaac gtaccggcag caccaatgct
     9421 gtcgagcagc gtcaggaagg tcgcgccgat aatcatattc gacacccagt tcgtggtggt
     9481 cgaacaggta ataccgaaat cgcggcattt cagcggctga atttcagagc acaggatcca
     9541 caccactggc gcggcgctca tcgcataacc ggcaatacac atcatcgtca tgccaacaga
     9601 gagccaggac aagccactgg aagccgtacc gttatcaaac tgcatcaggc aatagcccag
     9661 caccagagtg cctaacgcca tcacgctgaa accaattttc agagccggtt tacgccctgc
     9721 tttatctacc gtaaacaccg caataaaggt ggcgaacata aaggtcagcc ctacgaccag
     9781 agtcgcaatc atctgttgtt ctgtggtcgt aaagcccgcc attttgaaga tacgcggcgc
     9841 gtagtacatg atgatgttca taccggtaaa ctgctgcatc gcctgcaaca acataccgag
     9901 aaacacagca cgacggacgt tacggttgat cttaaacagt gcccaaccgc cctgttttaa
     9961 cttcaggctt tcacgaattt cgttgagttc ttctcgcgct ttttccgacg tatcgcgcag
    10021 catacgcaac acttcttccg cctcaatatg acgccccttt tccgccagcc agcgcgggct
    10081 atttggcagg aagaccacca gaataatcag caggactgct ggtaaagcaa gaacccccaa
    10141 cattgcgcgc cagttaccgc tataactgaa cgctgtatcg gataaaaacg ccagcacgat
    10201 gccgagtgtg accatcaact ggtacatact gatcatctta ccgcgaacgt tttcacttgc
    10261 catttcagaa agatacagag gagcggtgta agacgcgatc ccgacagcaa tgcccagcac
    10321 cacccgagcg gcgattaaca tctctacgct ggtcgcaaaa gcggacccta tagaaccgag
    10381 tacaaacagg atggcccccg ccatcaggct gtatttacgc cccaggcgga acgacagcca
    10441 accattaaac agcgcaccaa ttgctgcgcc gagcatcatg ctactaacca cccattcctg
    10501 caaacgactg gtcagcacaa agtgatcggt aatgaacggc aacgctccgg cgattacgcc
    10561 gatatcaaga ccaaataaca atcctgcgac cgcagcagct accgaaacaa acatattcat
    10621 acgccgcgta tcccgcaaag aacgtggcgt taaagcagat tccgtattga tagtaaccat
    10681 tttttcctgc cagcagagag taagacatag tgaaaaaata cgtgaacaac tcacacaggt
    10741 gtcaggtcgg aaacagcata aatatggatt aaattgctgc gacatgtcgt tatgtgatgg
    10801 atattccaat tttcaaatta agttgaatta ttgagattat tattaaccac ctaattttac
    10861 agcagataaa attcataaag ttcattaatt gataattgat atggattatt tcataaccat
    10921 gatatgaatt atgatgacct acaggtataa aaaaccctgc catgaggcag ggtcataaaa
    10981 gtaagaggaa tgaattaacg agccagccaa ccgccatcta cggcaatggt ataaccattc
    11041 acataatctg aagcgctgga ggcaaggaac actatcggcc ccatcaggtc actcggcagt
    11101 ccccaacgac cagctggaat gcggtcgaga atttccgcgc tacgttgttc atctgcccgt
    11161 agttgttgag tattgttggt cgccatgtaa cccggagcta tcgcattaac attaatgttg
    11221 tgtttagccc attcgttcgc catcaatcgc gtcacgccca tcacgccgct ttttgatgcg
    11281 gtataagaag gcacacggat cccgccctgg aaggagagca ttgacgtgat attgataatc
    11341 ttgccgccat tgcgttgcgc gataaagtgt ttcgccgctg cctgagacat gaagaatacg
    11401 ctcttgatat tcaggttcat gacatcgtcc cagtcttttt cgctgaactc gagagcatct
    11461 tcgcggcgaa tcaatccggc gttattcacc aggatatcaa tatgaccaaa ctcagctacc
    11521 gcgcgatcca gcagtgctgg aatgccatca atctttcgca gatcggcggt caggcttaaa
    11581 aaacgacgcc ccagcgccgt gacctgcttg atggtttcag tcggttcaac gatgttaatg
    11641 ccaacaatgt cacagcccgc ttgcgccagc cccaacgcca tcccctggcc cagtccagta
    11701 tcacaaccag tgacgaccgc aactttacct tcgagagaaa atgcacttaa aatcataaca
    11761 ataccttatt cgtttatgcc gacaactagc gcaaatcttt aacggccaca tggtccatat
    11821 catcaaagac ctggttttca ccaaccatgc cccagataaa ggtataagct ttggttccga
    11881 cgccggaatg gatcgaccag ctgggggaga tcaccgcctg ctcgttatgc atcacaatgt
    11941 gacgcgtttc ttgcggctgc cccatcatgt ggaaaacgca ggcgtcatca tccatattga
    12001 aatagaaata aacttccatc cggcgctcgt gagtgtgaca cggcatggtg ttccacaagt
    12061 tacctggagc cagctccgtc agccccatac tcaattggca ggtttccagc acatccggga
    12121 caaaatattt gttaattgtg cgacggttac tggtgaggtt atcgcctaac gtgactggag
    12181 atacttcgtc cggtgtgact tttttggtgg gatacgtcgt atgcgcgggt gcgcaattgt
    12241 aataaaactt tgccggagtg gcggtatcaa tactggcaaa gacaacttct tttgcacctt
    12301 taccaacata cagggcgtcg cggtgaccga tttcatagca ttggccatcg acagtaatcg
    12361 taccggcacc gccaatattg ataacaccta actcgcgacg ttcaaggaaa tagcttacgc
    12421 cgagttgttt accaacttcc ccgccaacgg aaaccgtttt agttatcggc ataatgccgc
    12481 caacaataat tcggtcaatg tggctgtaaa ccatggtgta ctcatcggcg acaaatacct
    12541 tttcaaccaa aaattcattg cgcagccctt gggtatccag cgtttttgcg tgcgcactgt
    12601 ggatgccctg tcttacgtcc acatcaacct ccgataatca gtgaagcgtg aaaatagaaa
    12661 acgatagtga aacactgttt cgttttctct ataaagggat gatatgccca tgggctaggg
    12721 ttttcaatta aaaataaaac aatgtttcat ttttaagtta aggattaaaa aaagtgcagt
    12781 gtcgatcacg aatatgtcat gagaatggaa aaatgaggga gcccagaaaa tgtatgtcga
    12841 cttgccagcc atcattattc aacagattaa tgacggcaga tatttatcgg taaggaggaa
    12901 atgcagagct actcgtcacg ttcaatggtc aatgccacgc cctggccccc gccgatacaa
    12961 agcgttgcca gccctttgcg ggcattacgt ttcaccattt catgaaccag agaaaccagg
    13021 attcggcaac cggaagcgcc tatcgggtga ccgagtgcga tcgcgccacc attgacattg
    13081 acccgacgct catcccactc aagcatcttg ccaaccgaaa gcgcctgtgc agcaaacgct
    13141 tcattagcct cgataagatc gacatcagcc aactgccagc ctacacgctc caggcaacgg
    13201 cgggtcgcat acaccggcgc aattcccatc aatgccggat ctacaccaac gctggcaaat
    13261 gcgcggatgc gggccagcac gggtaaattc aacgctcgtg ctttggcttc gctcatcatc
    13321 attaccgcag ctgcgccatc gtttatggat gatgcattcc ccgcggtcac agaaccgaga
    13381 ctatcaaatg aaggatttaa acgggctaaa ccttctgcac tggcgtcagt gcgtggctgt
    13441 tcatcggtat caacaaccaa cgtctgaccg ttactttgga tcattaccgg gacgatctca
    13501 tctttaaatc gtccggcgtc aatcgccgct cgcgcttttt gttgcgagct aagtgcgtaa
    13561 gcatcctgca actgacggct gatgccatat tcgcgagcca gattttcggc ggtgacacca
    13621 atatgataat cattgaaggc atcccacaac ccatcatgca ccagactgtc aaccaactgg
    13681 ctattgccaa gctgtgcacc ggtgcggcta tcagtcagaa catgtggtgc gcggctcatg
    13741 ttttcctggc caccggcgat gacaatatca gcctcgccac actgtatcgc ctgagtagcc
    13801 agatgcagtg ctttaagtcc ggaaccgcaa acgtcattaa tcgtgattgc agaaacgcta
    13861 ttaggaagac cacctttaat agccgattgc cttgccggat tctgccctgc ccctgcagtc
    13921 aacacctgac caagaattac ttcatccacc gcatatgcag gaacgccggt acgttctatt
    13981 aacgctttca cgaccagact accaagttcc acggcggaat gacccgctaa cgcaccacga
    14041 aagcagccga taggtgtccg taacgccccg acaatcacaa cgtctttcat cacaacctcg
    14101 caccaattaa cagtgcaata gtaaatgatt gttaattaca gttatcttaa ttgtttaaaa
    14161 aaagtgattt ttatcacaaa ggaaatatgc ctgagcagta gtcagagtca taactggcac
    14221 gtaaggtttg caaccactaa cccaccaata gatgggtaga taaggcgtta atctcccata
    14281 cttaacctgg tttatggtaa attgccctcc attttgttta atttgtagat gatacgttca
    14341 gataatgtct aatatttggt ctaaagaaga aactctgtgg agtttcgcgc tctacggcac
    14401 agccgttggt gcaggcacgc tcttccttcc tattcagtta ggttcggcag gggctgtggt
    14461 cctgtttatt actgctctgg tcgcctggcc tttaacatat tggccacata aagccttatg
    14521 ccagttcatc ctctcatcaa aaacatcagc atgtgaaggg ataacgggcg cggtaacaca
    14581 ctactatggc aagaagattg gtaatctgat taccacgctg tacttcatcg ccttttttgt
    14641 cgtcgtgttg atatatgcag tggcaattac caactcactt acggaacagc tggcaaagca
    14701 tatggttatt gatcttcgca tccgtatgtt ggtgagtctg ggtgttgtat taattctgaa
    14761 tctcattttt ctgatgggac gtcatgccac tattcgggta atgggatttt tggtattccc
    14821 attgattgcc tatttcttat ttctttccat ttaccttgtc ggtagttggc aacctgatct
    14881 attaacgacc caggtagagt tcaatcagaa tacccttcac cagatatgga tatcgattcc
    14941 cgtgatggtt ttcgccttta gccatacgcc cattatttct acgtttgcca tagacagacg
    15001 tgaaaaatat ggcgaacacg ctatggataa atgcaaaaaa attatgaaag tcgcttatct
    15061 catcatctgc ataagtgtac tgttctttgt ctttagctgc ctgctttcta ttccaccttc
    15121 gtatattgaa gcggctaaag aagaaggggt caccatttta tcggcgcttt ctatgctgcc
    15181 gaacgcccca gcatggttgt caatttccgg gattattgtc gcagtagttg cgatgtcgaa
    15241 atcattcctg ggtacgtact ttggcgttat tgaaggtgcc acggaggtcg tcaaaacaac
    15301 actacagcag gttggtgtaa agaaaagtcg tgcatttaac cgcgcactat caattatgtt
    15361 ggtatcgctg attaccttca ttgtttgttg cattaacccg aacgcgattt cgatgattta
    15421 tgcgatcagc ggcccgctca ttgccatgat acttttcatc atgcctacgt tgtcaacgta
    15481 tctgatcccg gcgcttaaac cctggcgctc catcggaaat ctgattacgc tgatcgtggg
    15541 tatcctgtgc gtatcggtaa tgttctttag ctaatatccg ttgtggatgg ttcaaaattg
    15601 ctacaatttt gaaccattcc atttttcata atattcattt gttattattc cgccctatca
    15661 cttctctcgt catatcctag aattatcagt tagttaaaca cacaaagaga tctgaacatc
    15721 ttaaataaga ctatttaaga tgcataactt agattcgcaa gatatcttct ggtcattcaa
    15781 aaacaatttg cgatatagat attctcatac catgagatag ttatctggac cacgttcgac
    15841 catgcaagat taaatcagtg aaacataatc atatttaata acgagaaatg catttttaaa
    15901 tgtcttaatc agaattatag caaatacaga ttaatcgcaa tttttcccag caaaattaca
    15961 aaatcaagat gtattgacat catcaatcac caatgaaatt atgcaattat atacggatag
    16021 ggaggttctt aacatggggc tttgtagtcg ttataaaagt cttacatgca atagttgctc
    16081 aatgcattgc caaataatgc cagaagagtc accgcgtttg cagtattgcg caaactcgtg
    16141 tttttgtatg tggcccgaag aaagttcata ttttaatcgt ggtgtggtgg aaggtatctt
    16201 aacaaaaaac cacaatgcga gattaagcgg atatattttt gtggattttt cagtaagttt
    16261 tttacgtcta ttccttgaaa aagactggat tgactatctt gcaagtactg acatggggat
    16321 agttttagtc agcgaccgca atatgcaatc gttagctaac tactggcgaa aacacaacac
    16381 cgcaatatct gctgtcatat ataatgatga tggtcttgat gttgccaatg aaaagatcag
    16441 acaactattt attggtcgtt atttatcatt taccagaggt aacacattaa ctcagatgga
    16501 attcaccata atggggtata tggtttctgg ttataatcca tatcagattg ctgaagtgct
    16561 ggatatggat atccgtagca tctacgcgta caagcaacga atcgaaaaga gaatgggtgg
    16621 taaaataaac gaattattta ttcgttcaca ttcggtccaa cattgatact caacgaccag
    16681 ccagaatcat actctgttca tacgggcatc tattgaaatg cccgtatttt ataaataagc
    16741 agtaacccca agagtaaaca acatagtatt taaaaccatc acacgaaaaa ttcagaagca
    16801 ttacgaaata tgaatttaca aacaatagtg gcataaatgt taaccatgtt aatttacgta
    16861 aagttttacg ttgcaacatt aaagcctcat ttcaatcatc atgataaata taaaattaat
    16921 atatatttat gccgtaaata ccaatatact tagcaaacta tgtgatctcc atttcgattg
    16981 atttagtgtt tattgacgta tgtactggat tattaacgat aatatcgagt tctggcctga
    17041 gcaccgaaaa ttaatatcgg tacataacgc cgatcttaac gtcgttctga caacgccagc
    17101 cagtcgatgt ttatcacttc tacttgaagc ttttcctgat gtggttacac aacaagattt
    17161 tttcaccaga gtctgggaag aagaaggtat gcgtgtgcct actaacacgt tatatcagaa
    17221 catatccatt atcagacgcg gatttcgcgc tgttggtgat actacccact cgctaattgc
    17281 aaccgtgccg agagaggatt caa
//
LOCUS       DAZPDU010000089        16922 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZPDU010000089 DAZPDU010000000
VERSION     DAZPDU010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16922
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.090"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            301..1353
                     /locus_tag="ACHURS_002605"
     CDS             301..1353
                     /locus_tag="ACHURS_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HFR1238298.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
     gene            complement(1669..2871)
                     /locus_tag="ACHURS_002606"
     CDS             complement(1669..2871)
                     /locus_tag="ACHURS_002606"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFR1238299.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAEDETGFTLDLNGMTVGCEKLVMATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPNVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            3103..4602
                     /gene="pitA"
                     /locus_tag="ACHURS_002607"
     CDS             3103..4602
                     /gene="pitA"
                     /locus_tag="ACHURS_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFR1238300.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            complement(4846..5181)
                     /gene="uspB"
                     /locus_tag="ACHURS_002608"
     CDS             complement(4846..5181)
                     /gene="uspB"
                     /locus_tag="ACHURS_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFR1238301.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            5572..6006
                     /gene="uspA"
                     /locus_tag="ACHURS_002609"
     CDS             5572..6006
                     /gene="uspA"
                     /locus_tag="ACHURS_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFR1238302.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            complement(6105..6158)
                     /locus_tag="ACHURS_002610"
     CDS             complement(6105..6158)
                     /locus_tag="ACHURS_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1238303.1"
                     /translation="MVLNVVLAGDFDCVRRS"
     gene            6324..7793
                     /gene="dtpB"
                     /locus_tag="ACHURS_002611"
     CDS             6324..7793
                     /gene="dtpB"
                     /locus_tag="ACHURS_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFR1238304.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRYGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(7842..8594)
                     /gene="rsmJ"
                     /locus_tag="ACHURS_002612"
     CDS             complement(7842..8594)
                     /gene="rsmJ"
                     /locus_tag="ACHURS_002612"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFR1238305.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(8602..10644)
                     /gene="prlC"
                     /locus_tag="ACHURS_002613"
     CDS             complement(8602..10644)
                     /gene="prlC"
                     /locus_tag="ACHURS_002613"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFR1238306.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTQALNDCRENVARVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKEVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            10847..11689
                     /gene="rlmJ"
                     /locus_tag="ACHURS_002614"
     CDS             10847..11689
                     /gene="rlmJ"
                     /locus_tag="ACHURS_002614"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFR1238307.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            11761..13113
                     /gene="gorA"
                     /locus_tag="ACHURS_002615"
     CDS             11761..13113
                     /gene="gorA"
                     /locus_tag="ACHURS_002615"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFR1238308.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(13167..13250)
                     /gene="dinQ"
                     /locus_tag="ACHURS_002616"
     CDS             complement(13167..13250)
                     /gene="dinQ"
                     /locus_tag="ACHURS_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFR1238309.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            13278..13451
                     /locus_tag="ACHURS_002617"
     CDS             13278..13451
                     /locus_tag="ACHURS_002617"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1238310.1"
                     /translation="MHPLTHPLPVTAHVSLLDDDYLTPARASVNGTTRTSDQDFESVY
                     AHYQSENASELTG"
     gene            13990..14343
                     /gene="arsR"
                     /locus_tag="ACHURS_002618"
     CDS             13990..14343
                     /gene="arsR"
                     /locus_tag="ACHURS_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFR1238311.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            14397..15686
                     /gene="arsB"
                     /locus_tag="ACHURS_002619"
     CDS             14397..15686
                     /gene="arsB"
                     /locus_tag="ACHURS_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFR1238312.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            15699..16124
                     /gene="arsC"
                     /locus_tag="ACHURS_002620"
     CDS             15699..16124
                     /gene="arsC"
                     /locus_tag="ACHURS_002620"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFR1238313.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(16258..16554)
                     /locus_tag="ACHURS_002621"
     CDS             complement(16258..16554)
                     /locus_tag="ACHURS_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFR1238314.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            16661..>16922
                     /locus_tag="ACHURS_002622"
     CDS             16661..>16922
                     /locus_tag="ACHURS_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR1238315.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAG"
BASE COUNT         3904 a         4388 c         4287 g         4343 t
ORIGIN      
        1 taatacttat atgttcgatg attttaagca ccgagactat ccggtctttt actaaataat
       61 ggaaaacaat ccatccctgg aagataattg taaaaaaaac aacagaataa gaattaatct
      121 taggataaat atttatttat catggccttt taaccggtaa aaaatggcac tcggcacagt
      181 aaaccccgct atagttatgc tatgcatttg tctttaatga tatctcgtcg ttatattaac
      241 aggctgaaat tatcattaat gcacatttca ttattagcag ggatacctga ggagtttaaa
      301 gtgaacatat atatcgggtg gcttttcaaa ttaatccctt tgattatggg attaatttgc
      361 atcgcgttag gtggctttgt gctggaaagt tcagggcaaa gcgagtattt cgtcgcgggt
      421 catgtgctga tttctctggc ggccatatgc ctggcattat tcactaccgc atttattatc
      481 atttcgcagc tcacgcgcgg cgttaatacg ttttacaata cattgttccc cattattggc
      541 tatgcggggt caattatcac catgatatgg ggttgggcac tgttagcagg caatgatgtg
      601 atggcagacg agtttgtcgc cggccatgtt attttcggcg ttggtatgat tgccgcctgt
      661 gtatcgacgg tggcagcgtc atccggtcac tttctgctca ttcccaaaaa tgcagcgggg
      721 agcaagagcg atggaacgcc ggtacaggct tattcttcat taatcggtaa ttgcctcatt
      781 gccgttcccg ttttactcac cctgctcggt ttcatttggt ctattacgct gttacgtagt
      841 gctgacataa ctccgcatta tgtcgcgggt cacgtattgc ttgggttaac cgcaatctgt
      901 gcctgtctaa ttggccttgt tgccacaatt gtccatcaaa cacgtaatac gttttcaact
      961 aaagaacact ggctgtggtg ttattgggtt atttttctcg gctcaatcac ggtactgcag
     1021 gggatatacg tcttagtcag ttccgatgca agcgcccgac tggctcccgg cattattctt
     1081 atttgcctcg gaatgatctg ttacagcata ttctcaaaag tctggctact ggcactggtc
     1141 tggagacgta cctgttcgtt agccaacaga ataccgatga tacccgtctt cacctgcctg
     1201 ttttgccttt tcctggcatc atttcttgcg gaaatggcgc agaccgacat gggatatttt
     1261 attccttcgc gagttctggt cggtttggga gcggtatgct ttacgttgtt ctcaatcgtt
     1321 tcaatattag aagcgggttc tgctaaaaaa taattgcaac gtaccggata aaaccagcgt
     1381 tgaccatttg cgtaacgctg gtttttctta ggcatcatga aataacgcag cattaatgca
     1441 tagtggttaa ggataaaaaa acaacaaagt tactgtcttt ttcagcctgt tcatatttta
     1501 taaagatctg gccactgggt gagttttcaa tcacctttcc ttccacctta tattaagcct
     1561 ggagggtttc agttcacggg ctcattagaa aataatcaca agatagccac tatcaaaatt
     1621 agctcatttt taatgcggct ctaataattt tcatctttag gtaacaggtc aggacgactt
     1681 cgctgcaatc aaatcctgcg cacaagccca cgcactcgac cacgcccact ggaagttata
     1741 gccccccagc cagccggtaa cgtccatcac ttcgccgatg aagtacagcc caggcacttt
     1801 gcgcgcttcc atcgtccgtg aagagagttc gttggtgtcc acgccgccga gcgtcacttc
     1861 ggcagtgcga tagccttcag tgccgttggg ttgtacgcgc cagtcggtca atgtgctaat
     1921 cagtgcctgt tggtcacgca cgttgagctg ttttagcgaa acattcggga tttgcccgag
     1981 ttgctgtaag cgttcaacca accgcttcgg tagatgaacc gccagtgtgt ttttcaggct
     2041 ctgattcgga tgtgcgttac gctgctcatt caggaaggtt tcgagatcca catccggtag
     2101 cagattgata ctgacaaatt cacccggttg ccagtagctg gaaatctgca acaccgccgg
     2161 tccagacaag ccgcgatggg tgaagagtaa gttctcacgg aaaacgatgc cgttttcagc
     2221 ggtaatcacg gaaggcaccg ccacgcccgc cagcacctgt aactcttcga gcaacggttt
     2281 atgcagagtg aatggcacca gacccgcacg ggtcggcagc acgttgaggc caaattgttc
     2341 ggcaatctta taaccaaacg gcgacgcgcc cagccccggc attgacagcc caccggtcgc
     2401 catgaccagc ttttcgcaac cgacagtcat gccgttcaga tcaagcgtga agcctgtttc
     2461 atcctccgcc acactcagca cttcgctacg caatctgaag gtcacattgc ccttctcgca
     2521 ctcatccacc agcatgtcga caatctgctg cgcggagtca tcgcagaaga gttgccctaa
     2581 cgttttctcg tgccatgcga tgccgtgttt attgaccaga tcaatgaaat cccactgggt
     2641 aaaacgtgcg agtgcagact tacaaaaatg cggattctgg ctcagataag cgcctggttc
     2701 gacataaagg ttggtaaagt tgcagcgccc accgccagac ataaggattt tgcgccctgg
     2761 ttttttacca ttatcgatca gcagaacccg gcgtcctgcc tgacctgcca gcgcagaaca
     2821 gaacataccc gccgcaccag cgcctataat aatggcatca aacctttcca cgttgcgctc
     2881 ctcttagaaa aaacgggcgt gaattgtaaa gattcctcag tggtcgcacc agcatcaata
     2941 ttactaaaag gaagtatttg cctgaattat ataagataat tattttttga gtgaaatcca
     3001 tacaggaggc aaatcaaaaa aagtctatat ttcactttgc ccgcgccgcg aaagtcactg
     3061 ataatgcgcc gcgttcatgt cctcaaaatg gcgtaacgtc ctatgctaca tttgtttgct
     3121 ggcctggatt tgcataccgg gctgttatta ttgcttgcac tggcttttgt gctgttctac
     3181 gaagccatca atggtttcca tgacacagcc aacgccgtgg caaccgttat ctatacccgc
     3241 gcgatgcgtt ctcagctcgc cgtggttatg gcggcggtgt tcaacttttt gggtgttttg
     3301 ctgggtggtc tgagtgttgc ctatgccatt gtgcatatgc tgccgacgga tctgctgctt
     3361 aatatgggat cgtctcatgg ccttgccatg gtgttctcta tgttgctggc ggcgattatc
     3421 tggaacctgg gtacctggta ctttggttta cctgcatcca gctctcatac gctgattggc
     3481 gcgatcatcg ggattggttt aaccaatgcg ttgatgaccg ggacgtcagt ggtggatgca
     3541 ctcaatatcc cgaaagtatt aagtattttc ggttctctga tcgtttcccc tattgtcggc
     3601 ctggtgtttg ctggcggtct gattttcttg ctgcgtcgct actggagcgg caccaagaaa
     3661 cgcgcccgta tccacctgac cccagcggag cgtgaaaaga aagacggcaa gaaaaagccg
     3721 ccgttctgga cacgtatcgc gctgatcctt tccgctatcg gcgtggcgtt ttcgcacggc
     3781 gcgaacgatg gtcagaaagg cattggtctg gttatgttgg tattgattgg cgtcgcgcca
     3841 gcaggcttcg tggtgaacat gaatgccact ggctacgaaa tcacccgtac ccgtgatgcc
     3901 atcaacaacg tcgaagctta ctttgagcag catcctgcgc tgctcaaaca ggctaccggt
     3961 gctgatcagt tagtaccggc tccggaagct ggcgcaacgc aacctgcgga gttccactgc
     4021 catccgtcga ataccattaa cgcgctcaac cgcctgaaag gtatgttgac caccgatgtg
     4081 gaaagctacg acaagctgtc gcttgatcaa cgtagccaga tgcgccgcat tatgctgtgc
     4141 gtttctgaca ctatcgacaa agtggtgaag atgcctggcg tgagtgctga cgatcagcgc
     4201 ctgttgaaga aactgaagtc cgacatgctt agcaccatcg agtatgcacc ggtgtggatc
     4261 atcatggcgg tcgcgctggc gttaggtatc ggtacgatga ttggctggcg ccgtgtggca
     4321 acgactatcg gtgagaaaat cggtaagaaa ggcatgacct acgctcaggg gatgtctgcc
     4381 cagatgacgg cggcagtgtc tatcggcctg gcgagttata ccgggatgcc ggtttccact
     4441 actcacgtac tctcctcttc tgtcgcgggg acgatggtgg tagatggcgg cggcttacag
     4501 cgtaaaaccg taaccagtat tctgatggcc tgggtgttta cccttccggc tgcggtactg
     4561 ctttccggcg ggctgtactg gctctccttg cagttcctgt aatcgtacgc accaaaacga
     4621 gcgggtcagc tggcccgctt cagattgtga catagtgcgc tttgttcatg ccggatgcgg
     4681 cgtgaacgcc ttatccggcc tacaaaaaca tgcaaattca atagattgca gagattatgt
     4741 aggcctgata agcgtagcgc atcaggcaat tttgcgtttg taatcagtct cgagcgggtc
     4801 agtttactgg cccgcttttt ttatgcgtta ttattgctga agtgcttaat gccaaatcat
     4861 caatgcaatc aggctgacca ccaccagacc acacaatgcg ctggtcagaa taaactgccg
     4921 acgcacccgc tcacagcggc gaataaattc atcatcgtga tgatcgcgat aacgttgggc
     4981 atagatatac caaacgagac gcacctgttt gttgggttgg ccatgtgagg taaaaaagcc
     5041 ccctccatca acatattgat agagcaatgg atcgcagtta cgcagtacca ctaacaacgc
     5101 gcgtagtgat gagaaatagc gcgccatgtt aacaatgcaa acgacacata aagcccaaaa
     5161 taatgcgacg gtgcttatca tacctcctcc ccggcgacct gcccgcggag ttccaccccg
     5221 gggctaccgc tcccgatacg ctgccaatca gttaacacca ggtcctggag aaaccgcttt
     5281 tgtggtgacc aacatccgag cggctctata gatagtgtag gagatcaggt tgtttttttt
     5341 ccagaaggtt aaccactatc aatatattca tgtcgaaaat ttgtttatct aacgggtaag
     5401 caaggcggat tgacggatca tccgggtcgc tataaggtaa ggatggtctt aacactgaat
     5461 ctttacggct gggttagccc cgcgcacgta gttcgcagga cgcgggtgac gtaacggcac
     5521 aagaaacgct agctggccag tcatcgacaa ctttatggaa ggagtaacac tatggcttat
     5581 aaacacattc tcatcgcggt cgatctctcc ccggaaagca aagttctggt agagaaagca
     5641 gtctctatgg ctcgccccta caatgcgaaa gtttctctga tccacgtaga tgtaaactac
     5701 tctgacctat acaccgggct tattgatgtg aatctgggtg atatgcagaa acgcatctct
     5761 gaagagacac atcatgcgct gaccgagctt tccactaatg caggctaccc aatcactgaa
     5821 accctgagcg gcagcggcga cctgggccag gttctggtcg atgcaatcaa gaaatacgat
     5881 atggatctgg tggtttgtgg tcaccaccag gacttctgga gcaaactaat gtcttccgca
     5941 cgtcagctga tcaacaccgt tcacgttgat atgctgattg ttccgctgcg cgacgaagaa
     6001 gaataatcat ccccccacga cgtgttcctg aacgcccgca tatgcgggcg ttttgctttt
     6061 tggcgcgcct tgttacctga tgcagcgtaa accccttatc tggcctacga tctgcgtacg
     6121 caatcaaaat ctcctgccaa tacaacattt aacactatca tattttccat cattagtgtg
     6181 atcagctggt tattttctgt tgtaatagtg tattaatcta ttcaccgcat caatattaag
     6241 aatctctgac agatgtaaac tttttcgcgc gttatccctt acgcgttcat acttttcagg
     6301 atggtattgg aaggttaata aatatgaata caacaacacc catggggatg ctgcagcaac
     6361 ctcgcccatt tttcatgatc ttttttgtcg agttatggga gcgattcggc tactacggcg
     6421 tgcagggcgt actggcggtt ttcttcgtta aacagcttgg attctcgcaa gagcaggctt
     6481 ttgtcacttt tggtgctttt gctgcgctgg tctatggcct catttccatt ggcggctatg
     6541 tcggcgacca cctgctgggg accaaacgca ccattgttct cggagcactt gtgctggcga
     6601 ttggctactt catgaccggc atgtcgctac ttaagcctga cctgattttc atcgccctgg
     6661 ggactatcgc tgtcggtaac ggcctgttta aagctaaccc agccagcttg ctttcgaagt
     6721 gctatccgcc gaaagatccg cggcttgatg gcgcattcac cctgttctat atgtcgatca
     6781 atatcggctc gttgatagcg ttatcgctgg cccctgtgat cgctgataga tacggttatt
     6841 cagtcaccta caacctgtgc ggggcggggt taattatcgc attactggtt tacatcgcct
     6901 gtcgtggaat ggtgaaagac attggttctg aacccgactt caagccgatg agcttcagca
     6961 aactgttgta cgtattactt ggcagcgtgg tgatgatctt cgtatgtgca tggctgatgc
     7021 acaacgtaga agtcgccaat ctggtgctga ttgttctctc catcgtcgtc accatcatct
     7081 tctttcgtca ggcattcaag ctggataaaa ccgggcgcaa taaaatgttt gtcgcctttg
     7141 tcctgatgct cgaagcggtg gtgttttaca ttctctacgc ccagatgcca acgtcgctga
     7201 acttctttgc catcaacaac gtgcatcatg aaattctcgg cttttccata aacccggtca
     7261 gcttccaggc gcttaacccg ttctgggtgg tactcgccag cccaatactg gcaggcattt
     7321 acacgcatct gggtaacaaa ggcaaagacc tctcgatgcc gatgaaattt actctcggca
     7381 tgtttatgtg ctcactgggc tttttgacgg cggcagctgc gggaatgtgg tttgcggatg
     7441 cacaagggct gacatcgcca tggtttatcg tgctggtgta tttattccag agcctgggtg
     7501 aattgtttat tagcgccctt ggcctggcga tgattgctgc cctggtgccg cagcatttga
     7561 tgggctttat tctcgggatg tggttcctga cgcaggctgc cgcattcttg ctgggcggct
     7621 atgtggcaac atttaccgca gtgccggaca acattaccga tccgcttgag acgttacccg
     7681 tctataccaa cgtgtttggt aagattggcc tggttacgct gggcgttgca gtagtgatgc
     7741 tgttgatggt gccgtggctg aaacgcatga ttgcgacgcc ggaaagccat taattattct
     7801 tgcagaaagc agggtagcgt tatcgctacc ctgctttact tttacacggg cgtacctgca
     7861 tagatatcaa agcgatgccc tttagtcaca accgcgtttg gcgtggcgac attcgctagc
     7921 ggtggcgcgt agtccgggcg ctttactacc acgcgtttgg tcgccagcaa gcgtgcaggc
     7981 tccagcaatc catcggcatc aagatccggc cccaccagcg actgaaagac acgcatctct
     8041 tttttcacca gcgcgctttt ctgcttatgc gggaacatcg ggtcgagata gaccacctgc
     8101 gggcgtgggg taatatcggt tagcgccgtc aggctggagg cgtgaattaa ctgcaaacgc
     8161 tcctgcaacc agccgccgat ttccgcatcc gcataaccac gcgccaggcc gtcgtcgagc
     8221 agcgcggcaa caactggatt acgctccagc atccgcacgc ggcaaccgac tgaagccagc
     8281 acaaaggcat cgcgccccag tcctgcagtg gcatccacca catccggcaa ataatcgcct
     8341 ttaatgccca ccgctttcgc caccgcctca ccgcgaccac cgccgaattt gcgtcggtgc
     8401 gccatcgctc cgccaacaaa atcaacaaag atgccgccaa gttttggctc atcacgcttg
     8461 cgcaattcca gatgttccgg cgttaacacc agcgccatca ggttgtcttc atcgtgctcc
     8521 agcccccagc gggccgccag aacagataag gcgccgtctc cggcgcctgt ttcatcaatt
     8581 aagcagattt tcactgaatg atcagccctt aatgccgtaa tgctccagca tcgcatccag
     8641 ctgcggttca cgaccacgga agcgtttgaa cagatccatc ggctcttctg aaccgccacg
     8701 gctcagaatg ttgtcgagga acgactgccc ggtttcacgg ttgaaaatgc cctcttcctc
     8761 aaagcgcgag aaagcatctg ccgccagtac gtcagcccac aggtagctgt agtaacctgc
     8821 cgcataacca ccggcgaaaa tatggctgaa agcgtgcggg aaacggcccc aggacggaga
     8881 tggcaccacg gcaaccagtt tcttgatttc tgccagagtt tcgaggattt ttgccccctg
     8941 atccgggcgg aactcggcat gaaggcggaa atcaaacagg ccgaactcca gctgacgcag
     9001 aataaacagc gccgcctggt agttcttcgc cgccagcatt ttatccagca actctttcgg
     9061 cagcggttcg ccggtttcat agtgaccaga gataaacgcc agcgcctccg gctcccagca
     9121 ccagttttcc ataaactgac tcggcagttc gaccgcatcc cacggcacac cgctgatacc
     9181 ggaaacacca gcggtttcga tgcgggtcag catatggtgc aggccgtgac cgaactcgtg
     9241 gaacagggtg atcacttcgt cgtgagtgaa cagcgccggt ttaccattta ccgggcggtt
     9301 gaagttacaa gtcaaatacg cgaccggttt ttgcagagaa ccatcagctt tacgcatctg
     9361 gcctacgcag tcatccatcc acgccccgcc gcgcttgttt tcacgggcat acagatcgag
     9421 gtagaagctg ccgcgcagtt cgttattttc gtcatacagt tcgaagaaac gtacatccgg
     9481 atgccagaca tcaacctctt tacgctcttt agcggtgatg ccgtaaatac gtttcaccac
     9541 ttcaaacagg ccgttaaccg ctttgttttc cgggaagtac ggacgcagct gctcgtcgct
     9601 gatgctgtag aggtgctgtt tctgtttttc gctgtagtaa gcgatatccc acggctgcaa
     9661 ctcatcgaca ccaaattcgg ctttagcgaa ggcacgcagt tgctccagct ctttttcgcc
     9721 ttgcggacgc gcgcgttttg ccagatcggt taagaaatcc agcacctgct gcgggttttc
     9781 tgccatttta gtggcaaggg atttaaaggc gtagttttca aagcccagca gttgcgccag
     9841 ttcgtgacgc agcgcgagga tctcttccat caccttgctg ttatcccact taccggcgtt
     9901 cgggccttga tcggaggcgc gggtgctgta agcgcgatac atctcttcgc gcagagcctg
     9961 gttgtcgcag taggtcatta ccggcaggta gcttgggata tccagcgtca gcaggtagcc
    10021 ttccagctct ttcgcttcgg cctgggcttt tgccgcagcc agcgcgcttt ctggcatccc
    10081 cgccagcccc gcttcgtcgg taacgagttt ggtccagccc atcgtcgcat cgaggacgtt
    10141 gttgctgtac tggttgccca gttcagaaag acgggtagca atttcgccgt aacgctgctg
    10201 tttctctttc ggcagaccta tgccagagag ttcaaaatca cgcagggcgt tatcaaccgc
    10261 tttcttctgc gccgtgttca gcgtggcgta atgatcgcca tcgcgcaggt cgcggtacgc
    10321 tttatacagc ccttcatgtt gccctaccca ggtgctgtat tccgacagca gcggcagggt
    10381 ttgctcgtag gcttcacgca gttccgggct atttttcacc gagttcaggt ggctgaccgg
    10441 ggagaagata cgccccaaca catcgtccac ttccgccaac ggctggcaga gattttccca
    10501 ggtgtacggt gccccttgcg ctactacgcg cgccacattt tcgcggcaat cgttcagcgc
    10561 ctgagtcacg gctggaacga catgttccgg gagaatttta gaaaacggag gcaattcaaa
    10621 gggagtcagt aacggattcg tcattcgcgc agtcctggtt aaagaggtta aggaagcgct
    10681 caacaggcgc tttacataat gtgtgtagca tggggttaag tgtagtgaat ttcaatgaga
    10741 aacgttacgc tttcgcggcg gcggcctctt ttcggtatac tgtcctgata cgcttttgtg
    10801 cgccccgaat acgggccgat ttttacttac cggaacacct ttacccatgc tcagttatcg
    10861 ccacagcttt cacgctggca accacgccga cgtccttaaa cataccgttc agagcctgat
    10921 catcgagtcg ctgaaagaga aagataaacc gtttctctat ctcgataccc acgcaggggc
    10981 cgggcgttat cagttaggca gcgaacatgc cgagcgtacc ggcgaatatc tcgaaggtat
    11041 cgcccgtatc tggcagcagg acgatttgcc cgcagaactg gaggcgtaca tcaatgtggt
    11101 aaaacacttc aaccgtagcg gtcagttgcg ttactacccc gggtcgccgt tgattgctcg
    11161 ccagctactg cgcgaacaag acagcctgca actgaccgaa ctgcacccga gcgattaccc
    11221 gttgctgcgt tctgaatttc agaaagacag ccgtgcgcgc gtcgaaaaag ccgacggttt
    11281 ccagcagctt aaggccaaac ttccgccggt ttcccgccgt ggtttaatcc ttatcgaccc
    11341 gccgtatgaa atgaaaaccg actatcaggc ggtggtcagc gggatagcag aaggttacaa
    11401 acgtttcgcc actggtactt acgcactgtg gtatccggtg gtgctgcgtc agcaaattaa
    11461 gcgcatgatc cacgatctgg aagcgaccgg tattcgcaaa attctgcaaa ttgaactggc
    11521 ggtactgcca gacagcgatc gccgtggcat gaccgcttcc ggcatgattg tgattaaccc
    11581 gccgtggaaa ctggaacagc agatgaataa cgtgctgccg tggctgcaca gcaaactggt
    11641 tccggcaggc accgggcacg ccaccgtaag ctggatcgtg ccggagtaat tgcagccatt
    11701 gctggcacct attacgtctc gcgctacaat cgcggtaatc aacgataagg acactctgtc
    11761 atgactaaac actatgatta catcgccatc ggcggcggca gcggcggtat cgcctccatc
    11821 aaccgcgcgg ctatgtacgg ccagaaatgc gcactgattg aagccaaaga gctgggcggc
    11881 acctgcgtga acgtcggctg cgtgccgaaa aaagtgatgt ggcacgcggc gcaaatccgt
    11941 gaagcgatcc atatgtacgg ccctgattat ggtttcgata ccaccatcaa caagttcaac
    12001 tgggaaacgt tgatcgccag ccgtaccgcc tatatcgacc gtattcatac ttcctatgaa
    12061 aacgtgctcg gtaaaaataa cgttgatgta atcaaaggct ttgcccgctt cgttgatgcc
    12121 aaaacgctgg aggtaaacgg cgaaaccatc acggccgatc atattctgat cgccacaggc
    12181 ggtcgtccga gccacccgga tattccgggc gtggaatacg gtattgattc tgatggcttc
    12241 ttcgcccttc ctgctttgcc agagcgcgtg gcggttgttg gcgcgggtta catcgccgtt
    12301 gagctggcgg gcgtgattaa cggcctcggc gcgaaaacgc atctgtttgt gcgtaaacat
    12361 gcgccgctgc gcagcttcga cccgatgatt tccgaaacgc tggtcgaagt gatgaacgcc
    12421 gaaggcccgc agctgcacac caacgccatc ccgaaagcgg tagtgaaaaa tgccgatggt
    12481 agcctgacgc tggagctgga agatggtcgc agtgaaacgg tggattgcct gatttgggcg
    12541 attggtcgcg agcctgccaa tgacaacatc aacctagaag ccgctggcgt taaaactaac
    12601 gaaaaaggct atatcgtcgt cgataaatat caaaacacca atattgaagg tatttacgcg
    12661 gtgggcgata acacgggtgc agtggaactg acaccggtag cagttgcagc gggtcgccgt
    12721 ctctctgaac gcctgtttaa taacaagccg gatgagcatc tggattacag caacattccg
    12781 accgtggtct tcagccatcc gccgattggt actgttggtt taacggaacc gcaggcgcgc
    12841 gagcagtacg gcgacgatca ggtgaaagtg tataaatcct ctttcaccgc gatgtatacc
    12901 gccgtcacca ctcaccgcca gccgtgccgc atgaagctgg tgtgcgttgg gccagaagag
    12961 aagattgtcg gtattcacgg cattggcttt ggtatggacg aaatgttgca gggcttcgcg
    13021 gtggcgctga agatgggcgc aaccaaaaaa gacttcgaca ataccgtcgc cattcaccca
    13081 acggcggcag aagagttcgt gaccatgcgc taaatgttaa agggctaaga gtagtgtgct
    13141 cttagccctt aattacgttt ccgctatcag ttcagaagct gaagcagaaa gcggatcagt
    13201 tccagcagcg caattaacgc tccaagaacg atgattgctt tatcaatcac ccgttttctc
    13261 catgcgatgg agtgagaatg catccgctta ctcatccact gcctgtcacg gcgcatgtct
    13321 cattgttaga tgacgactat ctcactccgg ccagagcatc agttaacggc accacccgta
    13381 cttctgacca ggactttgaa agcgtttatg cgcattacca gagtgaaaat gcctcagagc
    13441 taactggata atcatacagt acattcaggt tataaaacca gcatgtcctt gcaatagttt
    13501 cagtatggta ttagcattga tgcgttagat gatgactatc tcactccagc cagagccacc
    13561 aactcagggc tgaagagtaa aaaaaccgac gcaaagtcgg tttttttaca tccggattcg
    13621 gacaaggctt aatatgacga tgacccagtg aaagtatata aatcgtcatt gcgatatata
    13681 ccgaagtgcc ccctccgcca gctgaagaaa tcgctaattc ttgcaatgtt agccactggc
    13741 taatagtatt gaaccgttag atgtgggcat tctcactcca gccagagcca ccaactcagg
    13801 ctgaagagta aaaaaccgac gcaaagtcgg tttttttacg tcctgattca aaccgccttt
    13861 caaatgaata gccaactcaa aattcacacc tattaccttc ctctgcactt acacattcgt
    13921 taagtcatat atgtttttga cttatccgct tcgaagagag acactacctg ccacaatcag
    13981 gagcgcaata tgtcatttct gttacccatc caattgttca aaattcttgc tgatgaaacc
    14041 cgtctgggca tcgttttact gctcagcgag ctgggagagt tatgcgtctg cgatctttgt
    14101 actgccctcg atcagtcgca gcccaagatc tcccgccacc tggcattgct tcgtgaaagc
    14161 gggctattgc tggaccgcaa gcaaggtaag tgggttcatt accgcttatc accgcatatt
    14221 ccagcatggg cggcgaaaat tattgatgag gcctggcgat gtgaacagga aaaggttcag
    14281 gcgattgtcc gcaacctggc tcgacaaaac tgttccgggg acagtaagaa catttgcagt
    14341 taaaaattta gctaaacaca tatgaatttt cagatgtgtt ttatccggga ggcattatgt
    14401 tactggcagg cgctatcttt gtcctgacca tcgtattggt tatctggcag ccgaaaggtt
    14461 taggcatcgg ctggagtgca acgctcggcg cagtactggc gttagttacg ggcgtggttc
    14521 atccgggtga tattccggtg gtgtggaata tcgtctggaa cgcgacggct gcgtttatcg
    14581 ccgtcattat catcagcctg ctgctggatg agtccggctt ttttgaatgg gcggcgctgc
    14641 acgtctcacg ctggggtaat ggtcgtggtc gcttgctgtt tacctggatt gtcctgctcg
    14701 gtgctgccgt tgccgccctg tttgccaatg atggcgcggc gcttattttg acgccgattg
    14761 tcatcgccat gctgctggct ttaggattca gtaaaggcac gacgctggcg ttcgtgatgg
    14821 cggccggatt cattgccgat accgccagcc tgccgcttat tgtctccaac ctggtgaata
    14881 tcgtttccgc tgatttcttt ggcctcggct ttcgcgaata cgcctcggtg atggtgccgg
    14941 tggatatcgc cgcgattgtt gccacgctgg tgatgctaca tctctatttt cgcaaagata
    15001 ttccgcagaa ctacgatatg gcgctgctga aatctcccgc agaagcgatc aaagatcctg
    15061 ctacgttcaa aactggctgg gttgttttac tgcttctgct ggtgggattt ttcgtcctgg
    15121 aaccgctcgg cattccggtg agcgccattg cagctgtggg cgcgctgata ttatttgtcg
    15181 tcgctaaacg cggtcatgcg attaatacgg gtaaagtcct gcgcggtgcc ccctggcaga
    15241 ttgtcatctt ctcgctcggc atgtatctgg tggtttatgg cctgcgcaat gccggattaa
    15301 cggaatatct ttctggcgta ctcaacgtgc tggcggataa cggcctgtgg gccgcgacgc
    15361 tcggcaccgg attcctcacc gccttcctct cttctattat gaacaatatg ccgacggtac
    15421 tggttggcgc gttgtccatt gatggcagca cggcatctgg cgttatcaaa gaagcgatgg
    15481 tttatgccaa tgtgattggc tgcgatttgg gaccgaaaat taccccaatt ggtagcctgg
    15541 ctacgctact ctggctgcac gtactttcgc agaagaatat gactatcagc tggggatatt
    15601 acttccgtac agggattatc atgaccctgc ctgtgctgtt tgtgacgctg gctgcgctgg
    15661 cgctacgtct ctctttcact ttgtaatgag atactgatat gagcaacatt accatttatc
    15721 acaacccggc ctgcggcacg tcgcgtaata cgctggagat gatccgcaac agcggcacag
    15781 aaccgaccat tatccattat ctggaaactc cgccaacgcg cgatgaactg gtcaaactca
    15841 ttgccgatat ggggatttcc gtacgcgcgc tgctgcgtaa aaacgtcgaa ccgtatgagg
    15901 agctgggcct tgcggaagat aaatttactg acgatcggtt aatcgacttt atgcttcagc
    15961 acccgattct gattaatcgc ccgattgtgg tgacgccgct gggaactcgc ctgtgccgcc
    16021 cttcagaagt ggtgctggaa attctgccag atgcgcaaaa aggcgcattc tccaaggaag
    16081 atggcgagaa agtggttgat gaagcgggta agcgcctgaa ataaagcggc gatatccact
    16141 gccgatagtt ggtaaagtgc gctttgttta tgtcggatgc gacgctaacg cgtcttatcc
    16201 gacctacaaa accgtgcaaa ttcaatatat ggcatgaaat atgtagagcg tagcgcatca
    16261 ggcaattttg ctatttacgc agcactcctc gcgtaagaat tcaattatcg cttccagcat
    16321 tgcgtattgc gaaacacaca tcagcgtccg actctcacgg ttttgtttga ccagtcctac
    16381 cgataccagt gctgatatat ggtggctgag tgtggagcca ggtatcccaa gctgcttttg
    16441 caactcaccg accggcaacc cttgttcccc ggctttgaca agatgtttaa atatgaacaa
    16501 acgggtcggg tgacctagct ctttcagcgc tttagcgact tcttccaact gcatatgaca
    16561 cccctctgtc tggttgatat ttcgataata ccagaaatat cttgatctga atttcatttc
    16621 gattattctg gaaatataga tttatccaat gaggttacgt atgagtagtt ggcttgcgat
    16681 gctgcaagat gccgcagaga tgtttgtgtt tctcgccgtc gagctttcgt tgctgtttat
    16741 agtgattagt gccggtgtca gcctgataag acaaaaggtg ccagaccata aaatccagca
    16801 gatgatgggg gccagaaaag ggagaggcta tctcctggct gctctgttgg gagccgttac
    16861 cccgttctgt agttgctcga caatacccat gttacgtgga ttgttgtcag cgaaagccgg
    16921 gt
//
LOCUS       DAZPDU010000090        16682 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZPDU010000090 DAZPDU010000000
VERSION     DAZPDU010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16682
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.091"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            85..1224
                     /gene="arnB"
                     /locus_tag="ACHURS_002623"
     CDS             85..1224
                     /gene="arnB"
                     /locus_tag="ACHURS_002623"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFR1238316.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1228..2196
                     /gene="arnC"
                     /locus_tag="ACHURS_002624"
     CDS             1228..2196
                     /gene="arnC"
                     /locus_tag="ACHURS_002624"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFR1238317.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2196..4178
                     /gene="arnA"
                     /locus_tag="ACHURS_002625"
     CDS             2196..4178
                     /gene="arnA"
                     /locus_tag="ACHURS_002625"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFR1238318.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPAEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGHRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4175..5065
                     /gene="arnD"
                     /locus_tag="ACHURS_002626"
     CDS             4175..5065
                     /gene="arnD"
                     /locus_tag="ACHURS_002626"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFR1238319.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            5065..6717
                     /gene="arnT"
                     /locus_tag="ACHURS_002627"
     CDS             5065..6717
                     /gene="arnT"
                     /locus_tag="ACHURS_002627"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFR1238320.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGFTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            6714..7049
                     /gene="arnE"
                     /locus_tag="ACHURS_002628"
     CDS             6714..7049
                     /gene="arnE"
                     /locus_tag="ACHURS_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFR1238321.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7049..7435
                     /gene="arnF"
                     /locus_tag="ACHURS_002629"
     CDS             7049..7435
                     /gene="arnF"
                     /locus_tag="ACHURS_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFR1238322.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7429..7695)
                     /gene="pmrD"
                     /locus_tag="ACHURS_002630"
     CDS             complement(7429..7695)
                     /gene="pmrD"
                     /locus_tag="ACHURS_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFR1238323.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(7805..9160)
                     /gene="menE"
                     /locus_tag="ACHURS_002631"
     CDS             complement(7805..9160)
                     /gene="menE"
                     /locus_tag="ACHURS_002631"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFR1238324.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     D"
     gene            complement(9157..10119)
                     /gene="menC"
                     /locus_tag="ACHURS_002632"
     CDS             complement(9157..10119)
                     /gene="menC"
                     /locus_tag="ACHURS_002632"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFR1238325.1"
                     /translation="MRSAQVYRWQIPMDAGMVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQIVLLAWVNNWLAGDGELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVLEQLL"
     gene            complement(10119..10976)
                     /gene="menB"
                     /locus_tag="ACHURS_002633"
     CDS             complement(10119..10976)
                     /gene="menB"
                     /locus_tag="ACHURS_002633"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFR1238326.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(10991..11749)
                     /gene="menH"
                     /locus_tag="ACHURS_002634"
     CDS             complement(10991..11749)
                     /gene="menH"
                     /locus_tag="ACHURS_002634"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFR1238327.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VYLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILNFWLVGYSLGGRVAMMAACQGMPG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11746..13416)
                     /gene="menD"
                     /locus_tag="ACHURS_002635"
     CDS             complement(11746..13416)
                     /gene="menD"
                     /locus_tag="ACHURS_002635"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFR1238328.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     SGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLILIVVNNNGGQIFSLLPTPKSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETALADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13505..14800)
                     /gene="menF"
                     /locus_tag="ACHURS_002636"
     CDS             complement(13505..14800)
                     /gene="menF"
                     /locus_tag="ACHURS_002636"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFR1238329.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAREFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADNVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14879..15184)
                     /gene="elaB"
                     /locus_tag="ACHURS_002637"
     CDS             complement(14879..15184)
                     /gene="elaB"
                     /locus_tag="ACHURS_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFR1238330.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15239..15700)
                     /locus_tag="ACHURS_002638"
     CDS             complement(15239..15700)
                     /locus_tag="ACHURS_002638"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1238331.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLDT
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            15765..16682
                     /gene="rbn"
                     /locus_tag="ACHURS_002639"
     CDS             15765..16682
                     /gene="rbn"
                     /locus_tag="ACHURS_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFR1238332.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
BASE COUNT         3849 a         4543 c         4286 g         4004 t
ORIGIN      
        1 atttcgctcc aaatggcaaa atatacacaa cactctttat agcaaatata agtggacagg
       61 tattcaatgg cggaaggaaa agcaatgtca gaatttttgc ctttttcgcg accagcaatg
      121 ggcgtggagg aactcgctgc agttaaagag gttctcgaat ccggttggat cacaaccggt
      181 ccgaagaatc aggcgcttga gcaagccttt tgccagttga cgggaaatca gcatgccatc
      241 gcggtcagtt cagccaccgc cggaatgcat atcacgctaa tggcgttgga aattggcaag
      301 ggcgatgaag tgattacgcc ttccctgacc tgggtttcaa ccctcaatat gatttccttg
      361 ttgggtgcaa cgccggtaat ggtggatgtc gaccgcgata cgctgatggt cacgcctgaa
      421 gcgatcgagt cagccattac gccacgcact aaagccatca ttccggtgca ttatgccggt
      481 gcgccagcag atattgacgc cattcgcgcc attggcgaac gttacggcat cgcagttatc
      541 gaagatgctg cccatgccgt cggtacgtat tacaaagggc gacatattgg cgcaaaaggt
      601 accgctattt tttcatttca tgccattaaa aatattacct gtgcggaagg tggcctgatt
      661 gtaactgata atgaaaacct tgcccgccag ctacggatgc tgaaatttca cggtctgggt
      721 gtcgatgcct atgacagaca aacctggggc cgtgcaccgc aggctgaagt cttaacaccg
      781 ggctataagt acaatctgac cgatattaac gccgcgattg ccctgacaca gttagcaaaa
      841 ttagagcacc tcaacacccg tcggcgcgaa attgcccagc aatatcagca agcactggca
      901 gctctcccct ttcagccatt aagccttccc gcctggccgc acgttcacgc ctggcatctg
      961 tttattattc gtgtcgatga acaacgttgt ggtatcagtc gcgatgcgtt gatggaagcg
     1021 ttaaaagaaa gaggcattgg taccgggtta catttccgcg ccgctcacac acaaaaatat
     1081 tatcgcgagc gttttcccac gctgtcgtta ccgaataccg aatggaatag cgaacgcatc
     1141 tgttctttgc cgctgttccc ggatatgact accgccgatg ccgaccgtgt catcacagcc
     1201 cttcagcaac tcgcaggaca ataagccatg tttgaaatcc accctgttaa gaaagtctcg
     1261 gtggttattc ccgtttataa cgagcaggaa agcttaccgg aattaatcag gcgcaccacc
     1321 acagcctgtg aatcgttggg gaaagagtat gagatcctgc tgattgatga cggcagtagc
     1381 gataattccg cgcatatgct ggtcgaagcc tcacaagcgg agaacagcca tattgtgtct
     1441 attttgctta accgcaatta cgggcaacat tcagcgatta tggcgggatt cagtcacgtt
     1501 actggcgact taattattac ccttgatgcc gatctccaga atccgccaga agaaatcccc
     1561 cgcctggtgg caaaagccga tgaaggttac gacgtggtag ggactgtacg ccagaaccgc
     1621 caggacagct ggtttcgtaa aacagcttcg aagatgatta acaggcttat tcagcgcacc
     1681 accggcaaag cgatgggtga ctacggttgt atgctgcgcg cctatcgccg tcatattgtc
     1741 gatgcgatgt tgcactgcca tgaacgtagc acctttatcc cgattctggc gaatatcttc
     1801 gcccgccgtg ccattgaaat tccagtacat catgccgagc gtgagtttgg tgaatccaaa
     1861 tacagtttta tgcgcctgat taatttgatg tacgacctgg tgacctgcct taccacaacg
     1921 ccgctacgta tgctgagtct gctcggcagc attattgcga ttggaggttt tagcattgcg
     1981 gtgctgctgg tgattttacg cctgaccttc ggaccacaat gggcggcaga aggcgtcttt
     2041 atgctatttg ccgtgctgtt tacttttatt ggcgctcagt ttatcggcat gggattgctc
     2101 ggtgaatata tcggcaggat ctacaccgat gtccgcgccc gcccccgcta ttttgttcag
     2161 caagttatcc gtccatccag caaggaaaat gaataatgaa aaccgtcgtt tttgcctacc
     2221 acgatatggg atgcctcggt attgaagctc tgctggctgc cggttacgaa attagcgcca
     2281 tttttaccca taccgataat cccgctgaaa aagcctttta tggttcggtg gctcgtctgg
     2341 cggcggaaag aggcattcca gtttatgcgc cggataacgt taatcatccg ctgtgggtgg
     2401 aacgcattgc ccaactctca ccagaggtga ttttctcttt ttattatcgc catcttattt
     2461 gcgacgagat tttgcagctc gctcccgcag gtgcatttaa tctgcacggt tcgctgttac
     2521 caaaatatcg tggtcgcgcg ccgctgaact gggtgctggt gaacggtgaa acggaaacgg
     2581 gcgttacatt gcaccgaatg gtaaaacgtg ccgatgctgg ggccattgtg gcgcaactgc
     2641 gcgttgccat tgcgccagac gatatcgcta ttacgctgca tcataaattg tgccatgccg
     2701 cgcgccagct actggaacag acattacccg ccattaaaca cggtaatatt ctggaaattg
     2761 cccagcgcga aaacgaagcc acctgttttg gtcacagaac gccagatgac agtttccttg
     2821 aatggcataa accggcatcc gtactgcaca acatggtacg tgccgttgcc gatccgtggc
     2881 cgggtgcctt cagctatgtt ggcaatcaga aattcaccgt ctggtcgtcg cgtgttcatc
     2941 ctcatgccag caaagcacag ccggggagcg tgatttctgt tgcgccactg ctgattgcct
     3001 gtggcgatgg cgcgctggaa atcgtcaccg gacaggcggg cgacggcatt actatgcagg
     3061 gctcgcaatt agcgcagatg ctgggcctgg tgcaaggttc acgcttgaat agccagcctg
     3121 cctgcaccgc ccgacgccgt acccgggtac tcatcctcgg ggtgaatggc tttattggca
     3181 accatctgac agaacgcctg ctgcgcgaag atcattatga agtttacggt ctggatattg
     3241 gcagcgatgc gataagccgt tttctgaatc atccgcattt tcactttgtc gaaggcgata
     3301 tcagtattca ttccgaatgg attgagtatc acgtcaaaaa atgtgatgtc gtcttgccgc
     3361 tggtggcgat agccacgccg attgaatata cccgcaaccc gctgcgcgta tttgaactcg
     3421 attttgaaga gaatctgcgc attatccgct actgcgttaa gtaccgtaag cgaatcatct
     3481 tcccgtcaac ttcagaagtt tatgggatgt gtagcgataa atacttcgat gaggaccatt
     3541 ctaatttaat cgtcggcccg gtgaataaac cacgctggat ttattcggtg tcaaaacaat
     3601 tacttgatcg ggtgatctgg gcctatggcg aaaaagaggg tttacagttc accctcttcc
     3661 gcccgtttaa ctggatggga ccacgactgg ataaccttaa tgcagcgcga attggcagct
     3721 cccgcgctat tacgcaactc attctcaatc tggtagaagg ttcaccaatt aagctaattg
     3781 atggcggaaa acaaaaacgc tgctttactg atatccggga cggtatcgag gcgttgtacc
     3841 gcattatcga aaatgcggga aatcgctgcg acggcgaaat tatcaacatt ggcaatcctg
     3901 agaacgaagc gagcattgag gaactgggcg agatgctgct ggcgagcttc gaaaaacatc
     3961 cgctgcgcca tcacttccca ccgtttgcgg gctttcgtgt tgtcgaaagt agcagctact
     4021 acggcaaagg atatcaggac gtagagcatc gtaaaccgag catccgcaat gcccgccgct
     4081 gcctggactg ggaaccgaaa attgatatgc aggaaaccat cgacgaaacg ctggatttct
     4141 tcctgcgcac cgttgatctt acggataaac catcatgacc aaagtcggct tacgcattga
     4201 tgtcgatacc tttcgtggca cccgtgaagg cgtgccacgt ctgctggaaa tcttgagtaa
     4261 gcataatatt caggccagca tttttttcag cgtcggcccg gacaatatgg gccgccatct
     4321 ctggcgactg gtgaagccac agtttttgtg gaagatgctg cgctcaaacg cggcatcgct
     4381 ttatggctgg gatattttac tggcaggtac ggcctggcca ggtaaagaga ttggtcatgc
     4441 caatgccgat atcattcgtg aagcggctaa acatcacgaa gtcggcctgc acgcctggga
     4501 tcaccatgcc tggcaagccc gtagcggtaa ctgggatcgg caaacaatga tcgacgatat
     4561 tgcacgcggt cttcgcactc tggaagagat tatcggtcaa ccggtaacct gttctgccgc
     4621 tgcgggctgg cgtgccgacc agaaggtgat cgaagcaaaa gaagcgttcc atttgcgcta
     4681 caacagcgat tgtcgtgggg ccatgccgtt ccgtccactg ctcgaatcag gaaaccctgg
     4741 cactgcgcaa attccggtga ccttacccac ctgggatgaa gtgattggtc gggatgtgaa
     4801 agcagaagat tttaacggtt ggttactcaa ccgcatcctg cgagataaag gcacgccggt
     4861 ttataccatt catgcagaag ttgaaggctg cgcttatcag cataattttg tggatctcct
     4921 caaacgcgca gctcaggaag gcgtgacatt ttgcccttta agcgaactgt tatcagagac
     4981 gttgccgctc ggacaagttg ttcgcggaaa tattgccgga cgtgaaggct ggctgggttg
     5041 ccaacaaatt gcgggtagtc gctgatgaaa tcggtacgtt accttatcgg cctcttcgcg
     5101 tttattgcct gctattacct gttaccgatc agcacgcgtc tgctctggca acccgatgaa
     5161 acgcgttatg cggaaatcag tcgagaaatg ctggcatccg gcgactggat tgtgccccat
     5221 ctgttagggc tacgttattt cgaaaaaccc attgccggat actggattaa cagcattggg
     5281 caatggctat ttggcgcgaa taactttggt gtgcgggcag gcgttatctt tgcgaccctg
     5341 ttaactgccg cgctggtgac ctggtttact ctgcgcttat ggcgcgataa acgtctggct
     5401 ctactcgcca cagtaattta tctctcattg tttattgtct atgccatcgg cacttatgcc
     5461 gtgctcgatc cgtttattgc attctggctg gtggcgggaa tgtgcagctt ctggctggca
     5521 atgcaggcac agacgtggaa aggcaaaagc gcaggatttt tactgctggg aatcacctgc
     5581 ggcatggggg tgatgaccaa aggttttctc gcccttgccg tgccggtatt aagcgtgctg
     5641 ccatgggtag cgacgcaaaa acgctggaaa gatctcttta tttacggctg gctggcggtt
     5701 atcagttgcg tactgacggt tctcccctgg ggactggcga tagcgcagcg ggagcctgac
     5761 ttctggcact attttttctg ggttgagcat attcaacgct ttgcactgga tgatgcccaa
     5821 catagagctc cgttctggta ctacgtgccg gtcatcattg ccggtagcct gccgtggctg
     5881 ggattactcc ccggtgcact gtacacaggc tggaaaaacc gcaagcattc cgcaaccgtc
     5941 tatttgttga gctggacgat aatgccgctg ctgtttttct ccgtcgctaa aggtaaattg
     6001 cccacctata ttctttcctg ctttgcatct ctggcaatgc tgatggcgca ttacgctttg
     6061 ctggcagcaa aaaataatcc tctggcgctg cggattaatg gctggattaa catcgctttt
     6121 ggcttcactg gcattattgc cacatttgtg gtctccccgt ggggaccaat gaacacgccg
     6181 gtgtggcaaa ccttcgagag ctataaagtc ttttgtgcct ggtcgatttt ttcgctatgg
     6241 gcatttttcg gctggtacac cttaacaaac gtcgaaaaga cctggtcttt tgccgcgctt
     6301 tgcccgctgg ggctggcgtt gctggtagga ttttcaattc ctgacagagt catggaagga
     6361 aaacatccgc aattttttgt cgagatgaca caagaatcac ttcagccaag ccgctatatt
     6421 cttactgata gcgtcggtgt tgccgcaggt ctggcatgga gcctgcaacg cgatgacatc
     6481 atcatgtatc gccagacagg tgagttgaaa tacggcctta attatccgga tgcgaaaggg
     6541 agatttgtca gcggtgatga gttcgcaaac tggcttaatc aacatcgtca ggaggggatt
     6601 attacactcg tgctttcggt tgaccgcgat gaagatatca acagtctcgc cattccgccc
     6661 gcagatgcca tcgatcgtca ggagcgtctg gtgctgattc agtatcgtcc caaatgatct
     6721 ggctaacatt agtctttgcc agcttgctta gcgttgccgg gcagttgtgt cagaaacagg
     6781 caacctgctt tgtggcgata aacaaacggc gcaaacatat cgtgctgtgg ctgggactgg
     6841 cgctggcttg tcttggtctt gccatggtgc tctggctgct ggtcttgcag aacgtaccgg
     6901 taggcattgc ctacccgatg ttaagtctga attttgtctg ggtgacgctg gctgcagtaa
     6961 aactgtggca cgaaccggta tcgccgcgtc actggtgtgg ggtggcgttc attattggcg
     7021 gcattgtgat cctcgggagt acggtgtaat gggcctgatg tggggattat tcagcgtcat
     7081 tattgcctca gttgcgcaat taagcctggg ttttgcggcg agtcatctgc cgccgatgac
     7141 gcacttatgg gattttatcg cggctctgtt agcttttggc ctggatgccc ggatactgtt
     7201 actcgggctg ctgggttatc tgctttccgt tttttgttgg tataaaacgc tacataaact
     7261 tgcgctaagc aaagcctatg ccttgctcag tatgagttat gtgctggtat ggattgcttc
     7321 aatggtactt cccggctggg aagggacttt ttcgctgaaa gcactactgg gagtagcctg
     7381 tattatgagc gggttgatgc tgattttcct gcccacgaca aaacaacgtt actgagtttt
     7441 ccctgccact ttacactgcc gttcccactc atcggggcta taactgcttg ccgagaggac
     7501 tttcaccgta tgcagctttt cacgattaat acaataaagc gcattctgca aaggcgagag
     7561 taaatccccc actttcacgg cgaagtcact cttcacttcg gcgatcattt ttatcgcgcc
     7621 gccagcatcg cagagcataa gtacatgtct gttatcttgt ttattgcagc acgatttttt
     7681 gaccagccat tccattgcat tatcctgttt gctaagagtt ttcacatcaa tttacagatt
     7741 aacagataaa cctattatca acggcaatgg aatggcaaaa ttgcgaagtg aaactaatga
     7801 tttcttaatc ctggcgttgc acccactctt ttaacgcctg acgtgaaatt ttaataccgc
     7861 cgtttttcag ctccggcggt aaagttagcc agcgcaccgg ttgttgaaaa cgagccagct
     7921 tatctttcac ccattcgcca agatcaacgg tttgctggtc atactccacc accgctaccg
     7981 gtcgatgacc aaactccttg tcggcaacgg ggacgataaa cacctgcaaa accgcaggat
     8041 gtgcagcaat tacgcgctcg acttcttccg gctgaatacc ctctccgcca ctgaagaaca
     8101 gattgtctaa acgtccgaca atggtcagct tgccattatg catctcaccg cgatcgcgcg
     8161 tagcgtacca gccttcgtca ttaaccagtg aaaccagttg cccgttacgc cagtaacctt
     8221 ctgccatact ggcagcccgc agccacactt cattattaac gattttcact tcccgacccg
     8281 gcagcggcga accaacgtct gccaggccgt cggcttcttt cgcacacacc gtggaggcaa
     8341 actcggtcag accatagccg caaaagcaac gaatcccctg ctcgcgcgcc tgttccgtca
     8401 actcgaccgg gatagccgcg ccgccaagta acaccgcttt cagggaaacg gaactgcggt
     8461 taaccagcaa acgccagagt tgtgttggca ccagtgaagc gtgagtacag cctgccagca
     8521 tttgctccaa tggctgttta tcacgcaccg tcatccgcgc accagcgtat aaccagcgcc
     8581 acataattcc ctgaccggag acgtgaaaca gcggtaaaga gagcaaccag tcatcgtgat
     8641 cgccaaacgg aatcagcgat aacacacctt gcgcactggc aagatgggcc tgataagtat
     8701 ggacagcggc tttcggcaaa ccggtagaac ccgaggtcaa cgtcattgag cacagacgcg
     8761 tcggctgcca cgtagcggca tgtgcgcctt caacccgctg aatgtgcagc gacgttaatg
     8821 ccggaaacgt gttttcccca tccggcacca gcgcaaattg cagcgtcaga ttgggcagca
     8881 attcttcaag caacggttgc ggcagctgag ggttcacggg caacacccgc gccccgcatt
     8941 gcagtaacgc cagccaggcg agcagcgttt gcggcgtatt ccacgcccgc aacatcacgc
     9001 cgctgccctc aaccacaccc tgcgccgcaa agcccgtagc taactcatcg acgcgagcac
     9061 aaagctcgcg ccagttgagt tgctcgtcat taagacgtaa ggcgatggct tctccccgca
     9121 cttgccgcca gtgacgccac ggccagtcag agaagatcat aacaactgct ccagtacatc
     9181 agcgtccact aacggcagtg ggctccccgg ccagcgacga acctgctgcg cctgcatcag
     9241 atccagcgtg tccagtcccg gaatggtgtc tggcgttaac caggcagcaa tccgcgccag
     9301 ttgcgttaga cctaagctcg actcaatgga agaactgatc accgccgtca gccccagcgc
     9361 atgcgccgcc tgtacctgct cgcgtacttt ttccagactg cccgtgagcg tgggtttgat
     9421 aactaccgcg cgcacgccct cttcagccac aaaggcaaaa tccggctcgc gcaggctttc
     9481 atcccaggca atggcaatgc cggtttcacg ggcaaacgct cgcgaatcat cgcgggtttt
     9541 gcacggctct tcgagaaacg cgatgcggtg gcgataatcc gggttaacat atttggcaaa
     9601 ctgctgacct ttcagcggtg tccaggcgcg atttgcgtca agacgcaaat gcagatccgg
     9661 aattgcctcc agcaacagat taaccaccat gccgtcgcgc acggcttcgt acaatcccac
     9721 tttgaccttc gccactttct cgcctggcat atcagcaagt ttgaggatca gatcgtcagg
     9781 atcgccatta cacagtggtg ccgcacggta attggccgcc tgcgggagcg tatctgccag
     9841 ctccgccaat gcacagctta cgccaaaggc aacggaaggc atctgcggta gctcgccatc
     9901 gcctgccagc cagttattta cccaagcaag cagcacaatt tgcgcctctt cccaggtttc
     9961 ctgactgaag cccggcagtg gggagatctc cccccaccct tcgcgctcgc cttcacgcag
    10021 gcaaacatac agcccgtcgc gggtttttaa ccgcctgtcg cgcagaacca tccccgcgtc
    10081 catggggatc tgccagcggt atacctgcgc gctacgcatt acggattccg tttaaatttg
    10141 ctgaaatcag gctgacgttt ctggttgaaa gcgttgcgac cttcctgacc ttcttccgtc
    10201 atgtagaaca gcatggtggc gttgcccgcc agctcctgca gacccgcctg accgtcacag
    10261 tcggcgttca gcgcagcttt caggcagcgc agcgccatcg ggctgttttg cagcatttcg
    10321 cggcaccaac ggacggtttc tttttccaga tccgccagcg gtaccacggt gttgacaagg
    10381 cccatatcca gcgcctgttt tgcatcgtac tgacggcaca ggaaccagat ttcacgcgct
    10441 tttttctgtc cgacgatgcg agccatgtag gaagcgcccc agccgccgtc gaaggaaccg
    10501 actttcgggc cagtctgacc gaagatggca ttatctgccg cgatagtcag gtcgcacatc
    10561 atgtgcagaa cgtgaccacc gccgatggag tagccagcca ccatcgcgac aaccggtttc
    10621 ggacaggtac ggatctgacg ctggaagtcc agcacgttca ggtgatgtac gccggaatca
    10681 tctttatagc cgccgtaatc accacgcact ttctggtcac caccggagca gaacgcttta
    10741 tcgcctgcgc cagtcagaat gatcacgccg acgttgtcgt cataacgcgc atctgccagt
    10801 gcctggatca tctcttttac cgtcagagga cggaaggcat tgcgcacctg cggacgatta
    10861 atggtgattt ttgcgatacc gtcggtggat ttttcataac gaatgtcctc aaaaccttcg
    10921 gagcagtcgt gccattcaac cggtgcgtaa agcattgctt catcaggata aatcatattg
    10981 tgtcctttag tcagaaacgc aagatctgcg ccagacttgc gataacgcca gcgggatttt
    11041 cccgatgcgc gttatgtccg gcgcgaggaa tgacatggca gtcggcagcc agttccgccg
    11101 ccaaggcgcg gaatttgctg tcacgttcac cacataaata ataaaacgca aatgtgcggg
    11161 cgctaaggtt agcacgtaaa tcaggctgga cggcgagaga agtcgcctcc agcatggcgg
    11221 caagggttgc gccattattg ttactgcgca gcgccaccag ctcccggcgt tgatcgtcat
    11281 tgagcgaggc aaaaacaggc tgttgatacc agtcggcaaa taccgccgtt aacggttctg
    11341 tgcgaaaacg ctgcgcccat tggcgatcgg aacgctgacg ttccgcgcgt tgttcagcat
    11401 tttgcaaccc aggatgcccg ccttcgacaa caaccccaca aagccccggc atcccctggc
    11461 aagccgccat catcgccacc cgtcccccaa gcgagtaccc caccaaccag aagttaagga
    11521 tgttgtaact aaccagggtt ttacgcagta agtcggtgac atcatcaaat ccatcgacgc
    11581 taatcgccgc cgaaccaccg tgacctggga gataaacata caaccgtgag tagtcggcaa
    11641 acgcctcgcc cacttcttgc cattcgtggc aatcgccgga aaaaccgtgc aaaaacacca
    11701 gccagggtaa acctggtttg ccgtgttttg cctgcgcgtg caggatcata aatggcttac
    11761 ctgcgccaga agttgctgga gcgtttgcgc accatcggtg tcgttaacca ccatttcaat
    11821 taccgtggtg gttggcgtgc gccaggcatc ggcaagtgcc gtttcaagtt cctgccagtt
    11881 ttgcggacga tgatatttca gctcgaacat cgcggcggcg tgctcaaaat ggacgttttg
    11941 cggcatcaga tagaaacgct cgcgctcgct tttcggcgtt ggcagcagcg agaaaatttg
    12001 cccgccgttg ttgttcacca caattaatat cagcggcgcg gaaacctgac gcaataacgc
    12061 cagcgcgttg agatcgtaaa gtgcggagag atcgcccaca atcgccagcg tcggtttgcc
    12121 gcttgcccgc tgaacgccgg cggcagtcga gagcagcccg tcgataccgc tggccccacg
    12181 gttgctgtac accgggtaac ctgccggaag ttgcgaaagc gcatcaatca gacgtaccac
    12241 caggctgtta ccgacaaaca attgcccctg ttcaggcaga tagtcgctga tgcgatgcgc
    12301 cagttgcgct tcgccaaacg catcgcgacg ggcaataacc gcctgcattg cctgttccgc
    12361 caggcgcggg atttcaacgc accagggctg gcgtttttct gccggatgca gctccagcca
    12421 gtcggcaata ttggcaatta agcggcgtcc acgatggtgc gccggatcaa gccgcccttc
    12481 gatgtcatcc acaatccagt actcttcggg ttcacagctt gcctgccatt gcaggagccg
    12541 tttgcccgtc aggctgcttc ccagttgcac cacaatttgc gcctgttgca gttcgctggt
    12601 cgctttggca ttgcctaacc agagatcggc acacggcagc ggctgtccgg tttgcgacag
    12661 cacgtcgcca atcagcggcc agccaagagt ttgcgcccac agtgcaactt ttttgccctc
    12721 ttccgcactc atgcgcccgg caaccaccac gccgcgtttt tgtcgccaga agaaccagtc
    12781 gcgctgtttt tcactttcca gacgaggcgc ttcacgcagc cacggtttgt cgtcctgcca
    12841 ccagtcgccc agccgctgtt gccagctaag cccggtgtcg tccatttcgc catacagcgg
    12901 ttcggcaaac gggcagttga tatggactcc accagaatga agcgtaccga gagcatggtc
    12961 gatggtagaa accagccaac gtgcggggat atcctgggtc gggcgcggca atgaaatact
    13021 gtgcgtgggg tgagaggcga acattcccgg ctgacgaatc gcctgattcg cgccgcagtc
    13081 aatcagctcc ggcgggcgat cggcggttaa caggatcagt ttttctccgg ttaacccagc
    13141 ttcaatcagt gccggataga gatttgctac cgccgtaccg gaggtcacaa tcaccgccac
    13201 cggctgcttg ctgactttcg ccagccccag cgccagatgc cccaacccac gctcatcgaa
    13261 atgggtgtgg tgaatgaatg cggaattctc cgccgccgct aacgttaacg gtgtagaacg
    13321 cgagccaggg gcgatacaga tgtgtctgac gccgtgacgc gttaatgctt ccagaatgac
    13381 cgccgcccag cgtcggttaa atgcgcttac tgacatgagt ttgtccggta tcaatattgc
    13441 ggctaagtat aaggagctac aaaaatagat tattgatatg aatcggtaat gatgcgactc
    13501 attactattc catctgtaat aaagtacgca gccctgccgc tttgttgtcg atttcctgcc
    13561 actcttgctc ggggtcggaa ccacggacaa tgcccgcgcc agcatataat cgcacgacat
    13621 tgccgctaat ttttgctgag cgcagggaaa cgcagaattc gctttgctgt aatgagagat
    13681 agcccgcaga accggcgtac cattctcggg tgaacggttc gtgacgggcg ataaactgtc
    13741 gcgccagatc gcgcggtaag ccagcaactg ctgccgttgg ctgcaactga tgtaaacaga
    13801 tcacattatc cgctttgttg agtgaagtcc agatacagcg gcgaagatgc tgcactttac
    13861 gcagacgcag tacctgcggc ggtaaaacat ccagcgtctg ggtatcggcc tgtaaccgtt
    13921 ggcagatatc ttccaccacc agcatattct cgcgctggtt tttatcatcc gccatcagcc
    13981 actcacctaa ctgctgcgcc tgcttatcat caggatgatt tgctaccgtt cccgccagcg
    14041 cttcagtacg cagcgctttg tcacgccgcc gccataaccg ttccggtgaa gagccaagaa
    14101 aagcattttc gccatcaaag gccatgtaaa aatggtagca attcagattc agtcgacgac
    14161 tggcagccat catcgccgcc gcgttgaccg gacttgcgaa atgcaggtca gttgcccgag
    14221 cgagcaccac tttgtcgagc tcaccttcgg cgatggtttt cgttgccagt tcgattaatt
    14281 gcgtccagcc cgttttgtcc ggccagtgtt gttctcgcgt ggtggttaaa tgtaacccag
    14341 gcaagggctt gatactcacc agtgtggcga taaattctct tgccttaatc gcatcgtgct
    14401 gaagggagct ttcgctgaat agcgtcagcc gcagcgtggc tttaccgcca cagcgtcgcc
    14461 attccaggcg tggtaaaagt aaattgccct gcgacgggtc aaacgcattc agcccccaaa
    14521 tgcgtaagtc ggcgtgttcc gggtgctggc gaagaaaacg ttgtgcctgg tccaacgacg
    14581 taaaacgggt aatcgcgccc aggacggcag cttcttcatc accattacgt tgttgccagt
    14641 agaattgcgg gtatgtttgc tgactggcca gccagctcaa ggcatcaaaa gcgtctttga
    14701 gagggaaagg aatatcgata acccgaatgc cgggtgtcgc cggaatctct tgcgacaaat
    14761 ggcgcagtag attttccagc gccgtagtaa gtgattgcac gcggacctct ccctgttaaa
    14821 agcctcacat tatacggggt actacaaaaa aaatgcagta ccccggtgta gggaggtttt
    14881 aacggcgtgc cagcaacagt cctagtacca gcccaacggc cgcgcccaca ccaattcctt
    14941 gccagggttt ttcgtggacg tagtcatcag cacgataaac tgcctgcttc gcccgatagt
    15001 aataactgtc tgaagcctgg ctaacccgtt ttttcacatc atccagcgct ttttctgcac
    15061 gcgctttcag ctcaacatat ttctgatcgg cgggatcgcc agaggagcgg agcacctctt
    15121 ccagtgtttc actaagcagc gtcaggtcgt catcgatacg tgtatcacca aactgattag
    15181 acattctcgt tctccatttg cgtaaaacct gtcactaact atagacaagg gttgttgatt
    15241 acgcctgaat tacctcgcgt gccatcccaa tgtgtgggat accatcttct tcatagacct
    15301 ccgtcacagg gataaaaccg aaactctggt agaagttttg cagatgcgcc tgcgccccga
    15361 ggtaaacagg cttatcaggc cagtgatgcg tgcaggtgtc gagggtttta ctcatcagtt
    15421 gctgccccac tttttcgcca cgcaacgctt cactgacaat cacccgacca ataacgaccg
    15481 gctcaagatc atcatcactt ttcagaatcc tcgcatatgc caccagttca tcatttttcc
    15541 aaccgagaat atggcgatta tcccccgtca aatcatcacc atcgatatcc tgataagggc
    15601 aattctgctc gacaacaaac accgcgcaac gcagttgtaa taaggcataa agctgggaaa
    15661 ccgataattc agagtgatgc agatcttgcc attcaatcat aatgctctcc ttttagatgt
    15721 atgccttata ctagccggaa tattttttga aacgtgatga actcatggaa ttaatttttt
    15781 taggtacttc agccggtgtg ccaacccgca cgcgcaatgt cacggcaata ttgcttaacc
    15841 tgcaacatcc gacccagagc ggactttggt tgtttgactg cggtgaaggc acccagcatc
    15901 agctactgca taccgccttt aaccctggga aactggacaa gatttttatc agtcaccttc
    15961 atggcgatca tctctttggt ttacccggct tgctgtgcag tcgttctatg tcaggcatta
    16021 tccaaccctt aacgatttat ggtccgcaag gtatccgtga atttgtggaa accgcgctgc
    16081 ggattagcgg ctcatggacc gattatccgc tggaaattgt cgaaattggc gctggcgaaa
    16141 ttctcgatga tggcctgcgc aaagtgaccg cttatccgat ggaacaccca ctggaatgtt
    16201 atggctatcg tattgaagaa catgataaac cgggtgcatt aaatgcccag gcattaaaag
    16261 ctgctggcgt gccgcctggc ccactgtttc aggaattaaa agcgggcaaa acaatcacgc
    16321 tggaagatgg aaggcagatt aacggcgcag attacttagc tgctccagtg ccaggtaaag
    16381 cgctcgctat tttcggcgat accggcccct gcgatgccgc acttgacctg gctaaaggtg
    16441 tcgatgtcat ggtgcacgaa gcgacgctgg atataaccat ggaagccaaa gccaatagtc
    16501 gcggccatag ctctacacgc caggctgcga cactagcccg tgaggctgga gtcggcaagc
    16561 taatcattac ccacgtcagc tcgcgctatg atgacaaagg ttgtcaacac ctgttacgtg
    16621 aatgcaggtc aattttcccg gcgactgaac tggcgaatga tttcaccgtg tttaacgttt
    16681 aa
//
LOCUS       DAZPDU010000091        16274 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZPDU010000091 DAZPDU010000000
VERSION     DAZPDU010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16274
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.092"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(105..1307)
                     /gene="ubiI"
                     /locus_tag="ACHURS_002640"
                     /gene_synonym="visC"
     CDS             complement(105..1307)
                     /gene="ubiI"
                     /locus_tag="ACHURS_002640"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFR1238333.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(1330..2508)
                     /gene="ubiH"
                     /locus_tag="ACHURS_002641"
                     /gene_synonym="visB"
     CDS             complement(1330..2508)
                     /gene="ubiH"
                     /locus_tag="ACHURS_002641"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFR1238334.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADYATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            complement(2505..3830)
                     /gene="pepP"
                     /locus_tag="ACHURS_002642"
     CDS             complement(2505..3830)
                     /gene="pepP"
                     /locus_tag="ACHURS_002642"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFR1238335.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNESEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIMNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            complement(3856..4434)
                     /gene="ygfB"
                     /locus_tag="ACHURS_002643"
     CDS             complement(3856..4434)
                     /gene="ygfB"
                     /locus_tag="ACHURS_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFR1238336.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            4602..4931
                     /gene="zapA"
                     /locus_tag="ACHURS_002644"
     CDS             4602..4931
                     /gene="zapA"
                     /locus_tag="ACHURS_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFR1238337.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            4973..5156
                     /gene="ssrS"
                     /locus_tag="ACHURS_002645"
     ncRNA           4973..5156
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHURS_002645"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            5177..5779
                     /locus_tag="ACHURS_002646"
     CDS             5177..5779
                     /locus_tag="ACHURS_002646"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFR1238338.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(5880..5939)
                     /locus_tag="ACHURS_002647"
     CDS             complement(5880..5939)
                     /locus_tag="ACHURS_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR1238339.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(6271..6330)
                     /gene="ibsC"
                     /locus_tag="ACHURS_002648"
     CDS             complement(6271..6330)
                     /gene="ibsC"
                     /locus_tag="ACHURS_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFR1238340.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            complement(6559..7791)
                     /gene="serA"
                     /locus_tag="ACHURS_002649"
     CDS             complement(6559..7791)
                     /gene="serA"
                     /locus_tag="ACHURS_002649"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFR1238341.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            complement(8047..8706)
                     /gene="rpiA"
                     /locus_tag="ACHURS_002650"
     CDS             complement(8047..8706)
                     /gene="rpiA"
                     /locus_tag="ACHURS_002650"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFR1238342.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            9136..10029
                     /gene="argP"
                     /locus_tag="ACHURS_002651"
     CDS             9136..10029
                     /gene="argP"
                     /locus_tag="ACHURS_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFR1238343.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            10232..12376
                     /gene="scpA"
                     /locus_tag="ACHURS_002652"
     CDS             10232..12376
                     /gene="scpA"
                     /locus_tag="ACHURS_002652"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFR1238344.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKQLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAADHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            12369..13364
                     /gene="meaB"
                     /locus_tag="ACHURS_002653"
     CDS             12369..13364
                     /gene="meaB"
                     /locus_tag="ACHURS_002653"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFR1238345.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            13375..14160
                     /gene="scpB"
                     /locus_tag="ACHURS_002654"
     CDS             13375..14160
                     /gene="scpB"
                     /locus_tag="ACHURS_002654"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFR1238346.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMMPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            14184..15662
                     /gene="scpC"
                     /locus_tag="ACHURS_002655"
     CDS             14184..15662
                     /gene="scpC"
                     /locus_tag="ACHURS_002655"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFR1238347.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(15659..>16274)
                     /locus_tag="ACHURS_002656"
     CDS             complement(15659..>16274)
                     /locus_tag="ACHURS_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR1238348.1"
                     /translation="IVIGIDNTYPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLF
                     DRQLDFIISPQHVSARVQELENLTISELPPLRLGFLVSRRYEERQEQELLQELPWLQM
                     RFQNRANFEAMIDANMRPCGINPTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNP
                     ATLKYFDAPHRPMYMQEYLYSIRNHRYTATMLQHIAEDRDGTNH"
BASE COUNT         3953 a         4383 c         4144 g         3794 t
ORIGIN      
        1 aacggaggtg aaattagttt atttcaaatg aggaaaatct cccggcgaaa aaaccgggag
       61 atgaaagtgt gatgggtatc aaataaacag cagaagagaa atttttaacg cagccattca
      121 ggcaaatcgt ttaatcccat tgcctggcgg ataagttgtg gtttaacgcc aggaagcgtg
      181 tcggccagtt tcaaaccaat atcacgcagc agttttttcg ccggattggt accggaaaac
      241 agatcgcgga atccctgcat accagccagc atcagcgccg cactgtgctt gcggctacgc
      301 tcataccggc gcaggtaaat gtactgcccg atgtctttcc cctgacgatg caaccgtttc
      361 agttcagcaa tcagctctgc agcatccata aagccaagat ttactccctg ccccgccagc
      421 gggtgaatgg tatgcgcggc gtcgcccacc agcaccagac ggtgcgcggc aaactggcgc
      481 gcataacgcc ccgtcagtgg gaacacctga cgcgcgctct caaccttgca taagcccagg
      541 cgattatcaa aagcgatatt taacgcgcga ttaaattcat cttcacttgc ctgctgcatc
      601 cgctgcgctt cctctggcga cagtgaccag acaattgagc aaagatgcgg atcgctaagc
      661 ggtaaaaagg ccagaatgcc ttcgccatgg aaaacctgcc gcgccaccgc atcatgcggt
      721 tcttccgtgc gaatggtcgc taccagcgcg tgatgctgat aatcccagaa agtcagcgga
      781 atatctgctt tgttgcgcaa ccaggaatta gcaccgtccg cgccaatcac cagacgcgcc
      841 gttaacatgc tgccatcttt cagcgtcagg aaagtttcat tttctcccca ggcgacctgc
      901 tgtaattctg cgggggccaa cagagtgata tctgacgact gctgcgcttt gttccacagc
      961 gcgtagtgaa tcactgagtt ttcaacgata tgcccaagat ggctatagcc catgctttga
     1021 tcgtcaaacg aaatgtgacc aaaactgtct ttgtcccaca cttccatacc gtgataacaa
     1081 ctggccctac gagagagaat gtcctgccag acgccaagac gggtgagtaa tttttcgctg
     1141 gcggcattga tagccgaaac gcgcagttgt ggtggtgcat ccgccgccag aggttccggt
     1201 acgcgctgct ccagtacggc aacgcgtaag ccgctcccct gtaagccgca ggcaaccgcc
     1261 agccccacca tgccgccacc aacaatggct acatcaacac tttgcattgt ttattcctta
     1321 aaaccgcctt caacgcgcca cccaaccgag ggtgcgctgc gccagcacat cgcgtgccgg
     1381 ggtgaataat tccatcgtca tcagtccgat gttgcgcccg acaaccagcg gtgcccaacg
     1441 gttggcaaaa agatgtacca ggctgtccgt gacgccgatg gttgcttcgc gatcgctctg
     1501 tcgacgctgc tgatagcggc acaatatgcc gtaatcaccg atgtcttctc cgcgctcatg
     1561 cgcctgagtc agggtttccg caaggctcat gacatctcgc ataccgaggt taaacccttg
     1621 cccggcaatc gggtgcagag tttgcgccgc attgcccacc agcacggtac gatgggtaat
     1681 agctctggcg gcgcgggtta acgccagcgg ataagcactg cgtttaccgg cgtgggtaat
     1741 ttttccaagt cgccagccaa aggccgactg gagttcacgg caaaacttct cgtcgctcca
     1801 cgacagcacc tcttcgcgcc gttccagtgg atgacaccag accagcgaac agcgtccgtc
     1861 agacatcggc aacatcgcca gcgggccatg ttgcgtgaag cgttcaaaag cgcggccttc
     1921 gtgcgcaacg gaagtcgcaa cgttggcaat cacggccagt tgttcgtaag gctcctgctg
     1981 ccagtcaacg ccgcacgcgg tggctaacgc tgaatgggtg ccatcagctg ctaccagcac
     2041 gcggcccgtc agcgtctcgc cactctccag cgtcacttca acgtgactct gagtacgggc
     2101 aacgttagcc acgcgatcag ggcaatgcag cgttacgcca ggtgctttac gcagcaatgc
     2161 aaacagccgt tgcccgacat tgtgcaattc gacaacctgt cccagcgccg ccagttggta
     2221 atcttctgcg gcgagggtga caaatccagc gtgaccacga tcgctgacat gcacggtagt
     2281 gatggcggtt gcgtaatccg ccagagattg ccagacgccg atgcgcgcca gttgctgaca
     2341 ggtccccgca gccagcgcaa tagctcgtcc atcaaagccc ggatgagcat gtgactctgg
     2401 cgcggtcgct tcaatcaaat gtaccggcag cgccccgtga cttaaccggg aaatagccag
     2461 cgccagcgtc gcgcccgcca tgccgccacc gacgatgatt acgctcattg ctttctcgca
     2521 gctgccatca acgcttcgat ttcttccggc tttttcacca cgctggcggt gaggttttcg
     2581 ttgccggttt cggtaatcac aatgtcgtct tcaatacgaa tgccgatacc gcgatattgt
     2641 tctggcacat ccgcatccgg cgcgatatac agaccaggct ctacggtcag taccatcccc
     2701 ggttccagaa tgcgcgagcg atcctgacca taaacgccaa cgtcatggac atccagtcct
     2761 aaccagtggc taaggccatg cataaagaaa ggacgatggg cgttctgagc gatcagttca
     2821 tcaacatcac ctttcaggat gccgagtttt accaggccgc taaccatgat gcgcaccact
     2881 tcaccagtga cttccagaat ggaagttccc ggacgataca ggcgcaggct ggtttcgaga
     2941 gactccagca caatgtcgta gatttcacgc tgggcctggg tgaatttgcc gttgaccggg
     3001 aaggtgcggg taatatcgcc cgcgtaacct ttgtattcac aacccgcgtc aatcaacacc
     3061 aggtcaccgt cgcgcagttc actctcgttt tcggtgtagt gcagaatgca gccgttttca
     3121 ccgctgccga caatggtgtt ataggacgga tagcgcgcac cgtggcggtt aaattcgtgg
     3181 tgaatttcgc cttccagatg gtactcgaac attcccggac ggcatttttc catcgcccgt
     3241 gtatgtgcca tggcggtgat ttctcccgcg cggcggagta cggcaatctc ttctggcgat
     3301 ttgaacaggc gcatttcatg aacaacagga cgccagtcga tcatcgttgc cggtgcggtg
     3361 agattttgcc gcgaaccttt acgcagtttt tccagcgcac tgttcatgat ttcatcagca
     3421 tatgcatatt cgccctgggc atggtaaacc acatccaggc cgttaagtag ttgataaagt
     3481 tgctgattga tttcgctgaa tgccagtgcg cggtcaacgc ccagtttctc tggcgcggca
     3541 tcctggccta agcgacggcc aaaccagatc tccgccgtca ggtcgcgaac gcggttaaac
     3601 agaacgctgt ggttatgagt gtcatcgctt ttaatcagca ccagcaccgc ttccgattcg
     3661 ttaaagccgg tgaagtacca gaagtcactg ttctgacgat aggggtattc gctgtcggcg
     3721 ctacgtgtta cttctggtgc agcaaaaatc agcgcggcgc tgccgggttg catttgctcc
     3781 accagggcct gacggcgacg ctgaaactct tgccgggata tctcactcat aacactctcc
     3841 ttacgttttt tgtttttagt gtagagtcgg tttttgtact tctggcgcgg tcggttgcgg
     3901 atgagtaaag gtgtcgtggc ataacagcgc ggcaacacga acgtattcga tgatctcttc
     3961 aagcgacatt tcaagctctt cctgatcttc gtcttcgtcg taacccagtt gcgcaatgtt
     4021 acgcagatcg tcgatagctt caccggtttc gccggtcact ttatccagct tcggttgcgt
     4081 aacgccaaga ccaagcagga agtgattgac ccaacctgcc aatgcatcag cccgatcgaa
     4141 aacgctgaca tcatcgccat caggcagata aagctgaaaa aggaagccgt catcctgcag
     4201 ggcatcgctg gtggcagagt gcattttacg cagtgcctgt gccagctcat gaccgaaagc
     4261 catgccttcg ttcgtcaggt cgtgaagtag cggcagccat gagctgtcat cgttaccgcc
     4321 acatatcatc ccactgatta aaccatgcat ctcggcaggg gtcagccccg tcccttgttg
     4381 gttcagatac tggttcattt cgttgtaacc aggcatttcg ttctgtatag acataagcat
     4441 tcgtcgtcaa agggaggaat attcatgata tgctaccact ttgggccctg gtggaccaga
     4501 aaagggcttg tctcttctca tcagggtagc tatagtgtcg ccccttcgca gaccatgggt
     4561 ctaaagacga aggcagcgca gtcaatcagc aggaaggtgg catgtctgca caacccgtcg
     4621 atatccaaat ttttggccgt tcactgcgtg tgaactgccc gcctgaccaa agggatgcgt
     4681 tgaatcaggc agcggacgat ctgaaccaac ggttgcaaga tctgaaagaa cgcactagag
     4741 tcacaaatac tgaacagttg gtcttcattg ccgcattgaa tatcagctat gagttagcgc
     4801 aagaaaaagc gaagactcgt gactacgcgg caagtatgga acagcgtatt cggatgctgc
     4861 agcagaccat agaacaagcg ttacttgaac aaggtcgcat caccgaaaaa actaaccaaa
     4921 actttgaatg acacttttcg gtttactgtg gtagagtaac cgtgaagaca aaatttctct
     4981 gagatgttcg caagcgggcc agtcccctga gccgatattt cataccacaa gaatgtggcg
     5041 ctccgcggtt ggtgagcatg ctcggtccgt ccgagaagcc ttaaaactgc gacgacacat
     5101 tcaccttgaa ccaagggttc aagggttaca gcctgcggcg gcatctcgga gattcccttc
     5161 ttatctggca ccagccatga cgcaactacc agaactccca ctgacattat cccgacaaga
     5221 aatccgcaaa atgattcggc aacgtcgtcg tgcgttaacg ccggaacaac agcaggaaat
     5281 gggtcaacaa gccgctaccc ggatgatgac ttatcccccg gtggtgatgg cgcatacggt
     5341 cgctgtattc ctctcttttg atggtgaact cgacacccag ccgctaatag aacaactctg
     5401 gcgcgccggt aagcgcgtat atcttccagt tttgcatccc tttagtgccg gtaatttgct
     5461 gttcctgaat taccatccgc aaagcgaact ggtgatgaac aggttgaaga tccatgagcc
     5521 aaaactggat gtgcgtgacg tgctacccct ttcccgatta gacgtgctga tcacaccgct
     5581 ggtcgccttt gatgagtacg gtcagcgcct gggaatgggc ggtggttttt atgatcggac
     5641 cttacaaaac tggcagcact ataaaacgca accggtgggt tatgcgcatg attgtcagtt
     5701 ggtggaaaaa ctccccgttg aagagtggga tatccctctt cctgcggtgg ttacaccgtc
     5761 gaaagtctgg gagtggtaag ggcgatacac ctgcatcgcc ctgattgaca tcgttgattc
     5821 tttaacctaa tttagtgagt aagggtaagg gaggattgct cctcccctgt gactgactgt
     5881 taataagctg ggaaacttat gagtaacagt acaatcagta tgatggcaag tcgcatcata
     5941 acccttctcc ttcaagccct cgcttcggtg agggctttac cgttacagcc ccatgctgcc
     6001 cagctatcgt aagctctctt taaataacca caacgcattg atctaccttt aaatcttttc
     6061 ttcgagcgtg ctcgaaaaac ccatgcaaaa cagagataat ttttgggaaa aaggatctat
     6121 tacggcagta aacgtgggcg atgaagtggc tatcaaaaag ttcggttcag ggcgatgcac
     6181 cccgcatcgc cctgattgac atcgttgatt ctttgaccta atttagtgag taagggtaag
     6241 agaggatgac tcctcccctg agactgactg ttaataagct ccgaaactta tcagtaacag
     6301 tacaatcagt atgatgacaa gtcgcatcat aacccttctc ctttaagccc tcgcttcggt
     6361 gagggcttta ccgttacagc cccatgctgt cccgccatcg taaatcccca ttaaataaac
     6421 acaacgcatt gatctgagtt tgatttattt tctggagcag actcgcaaag tagaatgcgc
     6481 aacgcggcaa cggtgtggag aagggataaa aaaacgggca agtcagtgac ctgcccgtta
     6541 attttcagag aaggggaatt agtacagcag acgggcgcga atggtacccg gaatagcttt
     6601 cattgcctgc agcgcttttt cggcaacgtc ttcgtcagct tcaatatcaa taactacata
     6661 acccatctgg gcggaagttt gtagatattg cgcggcgatg ttgacgccct gctcggcgaa
     6721 gattttgttc agcgcagtta gcacgcccgg acggttttcg tggatgtgca tcagacgacg
     6781 accaccatgc agtggcagcg agacttccgg gaagttcacc gcagagagcg ttgagccatt
     6841 gtcagaatac ttgatcaatt tacccgcaac ttccaggccg atattctcct gcgcttcctg
     6901 agtcgaaccg ccaatgtgtg gcgtcagaag gacgttgtcg aattcacaca gtggagaggt
     6961 aaatggatcg ctattggtcg ccggttccgt cgggaatacg tcgattgccg cccccgccag
     7021 atgtttgctc gccagcgcat cacacagcgc cggaatatcc accacagtac cgcgcgaggc
     7081 attaatcagc agcgagccgg gcttcattag cgaaatctct ttcgcgccca tcatattttt
     7141 ggtggacgga ttctctggta catgcagact caccacatcg ctcatattca gcaggtcaga
     7201 aagatgctgt acctgagtgg cgttgcccag cggcagtttg ttttcaatat cataaaagta
     7261 aacatacatt cccagcgatt cagccagaat gcccaattgc gtaccaatat gaccgtagcc
     7321 gatgataccc agctttttgc cgcgcgcttc aaaagaaccc gccgccagtt tgttccacac
     7381 gccacggtgc gctttagcat tggcttccgg cacgccgcgc aatagcagca gcagttcgcc
     7441 aatcaccagc tccgcaacag agcgcgtatt tgagaacggt gcgttaaata ccgggatccc
     7501 gcgctttgcc gccgcatcca gatcaacctg gtttgttccg atacagaaac agccaatagc
     7561 gaccagtttt tctgcggcgt tgatcacgtc ttcagtcaga tgggtacggg atcgcaggcc
     7621 gatgaagtgg gcatcgcgga tggattcttt taattgttca tcatccagcg cgcctttgtg
     7681 aaattcgatg ttggtgtaac cagctgcacg aaggctttcc agcgcctttt ggtgcacgcc
     7741 ttctaccagc agaaacttaa tcttgtcttt ctccagcgat acctttgcca tttacccaat
     7801 cctgtctttt gaaatgttgt gtgcggattt gcatccgcct ttcaacatat caaaaaataa
     7861 tattgcggca atatgaacgt ttgcgtcgcg atgttgagga aatatcacgc aaaggtattt
     7921 cttggttgaa tatgctggtt tagaggattt atttagagca atcgacaata gccaagtaaa
     7981 aacgtgacac atgtcaccaa atttaatgaa gagaattttt ttaacggggg aggttccccc
     8041 gtcagatcat ttcacaatgg ttttgacacc gtcaggtgtg ccaatcagcg caacgtccgc
     8101 gccacggtta gcaaacaagc caacagtcac cacgccagga atcgcattta tggcgttttc
     8161 catcgctatc gggtcaagga tttccatgcc gtggacgtcg aggatcacgt tgccgttatc
     8221 ggtcaccacg ccctgacggt attccggacg accgcccagt ttcaccagct ggcgcgccac
     8281 tgcactacgt gccatcggga taacttctac tggcagcggg aatttgccca gaatatcaac
     8341 ctgcttggaa gcgtcagcaa tacagataaa tttttccgca accgaagcaa tgattttttc
     8401 acgggtcagc gccgcgccgc cgcctttgat catttgcatg tggccgttga tttcatctgc
     8461 gccatcaacg tagatgccaa ggctgtcgac ttcgttgaga tcaaaaacgt gaatgccgag
     8521 gcttttcagt ttttcagtgg aagcatctga actggaaacg gccccttcaa tctggccttt
     8581 cattgtaccg agcgcgtcaa taaagtgtgc ggcggtggaa cctgtaccta caccaacaat
     8641 ggtgccgggc tgaacatact gaagtgccgc ccatcctact gcttttttca attcatcctg
     8701 cgtcatgatc gtttcgcctg tggtatgaaa tttcacacgc attatataca aaaaaagcga
     8761 ttcagacccc gttggcaagc cgcgtggtta actcatccat aaaatatcgc gcaatggcgg
     8821 gcatcccctt tcgccccgca aataaagcat acaacggtct gggtatgccg ctccacggtg
     8881 cgaacaggcg caccagttcc ccccgttcgc aagcccctgt ttacaggcaa attgaggcaa
     8941 taacgccacc ccattcaaca caaccagggc acgaactcgc tgagcgaaat gcattagcgc
     9001 aaatccctga tcttttaagc tgcgcgttta accacggata caaatgttac ccgccgacgg
     9061 cttcggtata tgcaacctga cacaaaattg tgtcatagtg caggaaaaag catttaccag
     9121 gagcagacaa cagcaatgaa acgcccggac tacagaacat tacaggcact ggatgcggtg
     9181 atacgtgaac gaggatttga gcgcgcggca caaaagctgt gcattacaca atcagccgtc
     9241 tcacagcgca ttaagcaact ggaaaatatg ttcgggcagc cgctgttggt gcgtaccgta
     9301 ccgccgcgcc cgacggaaca agggcaaaaa ctgctggcac tgctgcgcca ggtggagttg
     9361 ctggaagaag agtggctggg cgatgaacaa accggttcga ctccgctgct gctttcactg
     9421 gcggtcaacg ccgacagtct ggcgacgtgg ttgcttccgg cactggctcc tgtgttggct
     9481 gattcgccta tccgcctcaa cttgcaggta gaagatgaaa cccgtactca ggaacgtctg
     9541 cgccgcggcg aagtggtcgg cgcggtgagt attcaacatc aggcgctgcc gagttgtctt
     9601 gtcgataaac ttggtgcgct cgactatctg ttcgtcagct caaaaccctt tgccgaaaaa
     9661 tatttcccta acggcgtaac gcgttcggca ttactgaaag cacctgtggt cgcctttgac
     9721 catcttgacg atatgcacca ggcctttttg cagcaaaact tcgatctgcc tccaggcagc
     9781 gtgccctgcc atatcgttaa ttcttcagaa gcgttcgtgc aacttgctcg ccagggcacc
     9841 acctgctgta tgatcccgca cctgcaaatc gagaaagaac tagccagcgg tgaactgatt
     9901 gacttaacgc cagggctatt tcaacgccgg atgctctact ggcaccgctt tgctcctgaa
     9961 agccgcatga tgcgtaaagt cactgatgcg ttgctcgatt atggtcacaa agtccttcgt
    10021 caggattaat ccatcaaata atgcctgata gcacatatca ggcgttatcc tcacttcttt
    10081 ttgtattcct tgaatcacat cacaaaatag acaaatctca ggcggcaaaa aacgacgtct
    10141 gaatgcattt ttttgctggc gacaaaccca cgtaaaaagc tcaccgtaag cgcaaatacc
    10201 ctcattttga ttgcgtttta cggagcaaat aatgtctaac gagcaggagt ggcaacagct
    10261 tgccaacaag gaattgagcc gtcgggagaa aactgtcgac tcgctggttc agcaaaccgc
    10321 ggaagggatc gccatcaagc agctgtatac cgaagccgat ctcgataatc tggaggtgac
    10381 aggtaccctt cctggtttgc cgccctacgt tcgtggcccg cgtgccacta tgtataccgc
    10441 ccaaccgtgg accatccgtc agtatgctgg tttttcaaca gcaaaagagt ccaacgcttt
    10501 ttatcgccgt aacctggccg ccgggcaaaa aggtctttcc gttgcgtttg accttgccac
    10561 ccaccgtggc tacgactccg ataacccacg cgtggcgggt gacgtcggca aagcgggcgt
    10621 cgctatcgac accgtggaag atatgaaagt cctgttcgac cagatcccgc tggataaaat
    10681 gtcggtttcg atgaccatga atggcgcagt gctaccagta ctggcgtttt atatcgtcgc
    10741 cgcagaagag caaggtgtta cacctgataa actgaccggc accattcaaa acgatattct
    10801 caaagagtac ctctgccgca acacctatat ttacccacca aaaccgtcaa tgcgcattat
    10861 cgccgacatc atcgcctggt gttccggcaa catgccgcga tttaatacca tcagtatcag
    10921 cggttaccac atgggggaag cgggtgccaa ctgcgtgcag caggtagcat ttacgctcgc
    10981 cgatgggatt gagtacatca aagcagcaat ctctgccgga ctgaaaattg atgacttcgc
    11041 tcctcgcctg tcgttcttct tcggtatcgg catggatctg tttatgaacg tcgccatgtt
    11101 gcgtgcggca cgttatttat ggagcgaagc ggtcagtgga tttggcgcac aggatccgaa
    11161 atcactggcg ctgcgtaccc actgccagac ctcaggctgg agcctgactg aacaggatcc
    11221 gtataacaac gttatccgca ccaccattga agcactggct gcgacgctgg gcggtactca
    11281 gtcactgcat accaacgcct ttgacgaagc gcttggtttg cctaccgatt tctcagcacg
    11341 cattgcccgc aacacccaga tcatcatcca ggaagaatca gaactctgcc gcaccgtcga
    11401 tccactggcc ggatcctatt acattgagtc gctgaccgat caaatcgtca aacaagccag
    11461 agctattatc caacagatcg acgaagccgg tggcatggcg aaagcgatcg aagcaggtct
    11521 gccaaaacga atgatcgaag aggcctcagc gcgcgaacag tcgctgatcg accaaggcaa
    11581 gcgtgtcatc gttggtgtca acaagtacaa actggatcac gaagacgaaa ccgatgtact
    11641 tgagatcgac aacgtgatgg tgcgtaacga gcaaattgct tcgctggaac gcattcgcgc
    11701 cacccgtgat gatgccgccg taaccgccgc gttgaacgcc ctgactcacg ccgcacagca
    11761 taacgaaaac ctgctggctg ccgctgttaa tgccgctcgc gttcgcgcca ccctgggtga
    11821 aatttccgat gcgctggaag tcgctttcga ccgttatctg gtgccaagcc agtgtgttac
    11881 cggcgtgatt gcgcaaagct atcatcagtc tgagaaatcg gcctccgagt tcgatgccat
    11941 tgttgcgcaa acggagcagt tccttgccga caatggtcgt cgcccgcgca ttctgatcgc
    12001 taagatgggc caggatggac acgatcgcgg cgcgaaagtg atcgccagcg cctattccga
    12061 tctcggtttc gacgtagatt taagcccgat gttctctaca cctgaagaga tcgcccgcct
    12121 ggccgtagaa aacgacgttc acgtagtggg cgcatcctca ctggctgccg atcataaaac
    12181 gctgatcccg gaactggtcg aagcgctgaa aaaatgggga cgcgaagata tctgcgtggt
    12241 cgcgggtggc gtcattccgc cgcaggatta cgccttcctg caagagcgcg gcgtggcggc
    12301 gatttatggt ccaggtacac ctatgctcga cagtgtgcgc gacgtactga atctgataag
    12361 ccagcatcat gattaatgaa gccacgctgg cagaaagtat tcgccgctta cgtcagggtg
    12421 agcgtgccac gctcgcccag gccatgacgc tggtggaaag ccgtcaccca cgtcatcagg
    12481 cgctaagtac acagctgctt gacgccatta tgccgttctg tggtaacgcc ctgcgactgg
    12541 gcgttaccgg cacacccggc gcggggaaaa gtacctttct tgaagccttt ggcatgttgt
    12601 tgattcgtga gggattaaag gttgctgtta ttgcggtcga tcccagcagc ccggtcactg
    12661 gcggtagcat tctcggggat aaaacccgca tgaatgacct ggcgcgtgcc gaggcggcgt
    12721 ttattcgccc ggtaccctcc tccggtcatc tgggcggtgc cagtcagcga gcacgggaat
    12781 taatgctgtt atgcgaagcg gcgggttatg acgtagtgat tgtcgaaacg gttggcgtcg
    12841 ggcagtcgga aacggaagtc gcccgcatgg tggactgttt tatctcgttg caaattgccg
    12901 gtggcggcga tgatctgcag ggcattaaaa aagggctgat ggaagtggct gatctgctcg
    12961 ttatcaacaa agacgatggc gataaccata ccaatgtcgc cattgcccgg catatgtacg
    13021 agagtgccct gcatattctg cgacgtaaat acgacgaatg gcagccacgg gttctgactt
    13081 gtagcgcact ggaaaaacgt ggaatcgatg agatctggca cgccatcatc gacttcaaaa
    13141 ccgcgctaac tgccagtggt cgtttacaac aagtgcggca acaacaatcg gtggaatggc
    13201 tgcgtaagca gaccgaagaa gaagtactga atcacctgtt cgcgaatgaa gatttcgatc
    13261 gctattaccg ccagacgctt ttagcggtca aaaacaatac gctctcaccg cgcaccggcc
    13321 tgcggcagct cagtgaattt atccagacgc aatattttga ttaaaggaat ttttatgtct
    13381 tatcagtatg tgaacgttgc cactatcaac aaagtggcgg tcattgagtt taactatgga
    13441 cgaaaactta atgccttaag taaagtcttt attgatgatc ttatgcaggc gttaagcgat
    13501 ctcaaccggc cggaaattcg ctgtatcatt ttgcgggcgc caagtggatc caaagtcttc
    13561 tccgcaggtc acgatattca cgaactgccg tctggcggtc gcgatccgct ctcctatgat
    13621 gatccattgc gtcaaatcac ccgcatgatc caaaaattcc cgaaaccgat catttcgatg
    13681 gtggaaggta gtgtttgggg tggcgcattt gaaatgatca tgagttccga tctgatcatc
    13741 gccgccagta cctcaacctt ctcaatgatg cctgtaaacc tcggcgtccc gtataacctg
    13801 gtcggcattc acaacctgac ccgcgacgcg ggcttccaca ttgtcaaaga gctgattttt
    13861 accgcttcgc caatcaccgc ccagcgcgcg ttggctgtcg gcatcctcaa ccatgttgtg
    13921 gaagtggaag aactggaaga tttcacctta caaatggcgc accacatctc tgagaaagcg
    13981 ccgttagcca ttgctgttat caaagaagag ctgcgtgtac tgggcgaagc gcacaccatg
    14041 aactccgatg aatttgaacg tattcagggg atgcgccgcg cggtttatga cagcgaagat
    14101 taccaggaag ggatgaacgc tttcctcgaa aaacgtaaac ctaatttcgt tggtcattaa
    14161 tccctgcgaa cgaaggagta aaaatggaaa ctcagtggac aaggatgacc gccgatgaag
    14221 cggcagaaat tatccagcat aacgacatgg tggcatttag cggctttacc ccagcgggtt
    14281 cgccgaaagc cttaccgacc gcgattgccc gcagagctaa cgaacagcat gaggccaaaa
    14341 agccgtatca aattcgcctg ctgacggggg cgtcaatcag cgccgccgct gacgatgtac
    14401 tttctgacgc cgatgctgtt tcctggcgtg cgccatatca aacatcgtcc ggtttacgta
    14461 aaaagatcaa tcagggcgcg gtgagtttcg ttgacctgca tttgagcgaa gtggcgcaaa
    14521 tggtcaatta cggtttcttc ggcgacattg atgttgccgt catcgaagca tcggcactgg
    14581 caccggatgg tcgagtctgg ttaaccagcg ggatcggtaa tgcgccgaca tggctgctgc
    14641 gggcgaagaa agtgatcatt gaacttaatc actatcacga tccgcgcgtt gcagaactgg
    14701 cggatattgt gattcctggc gcaccaccgc ggcgcaatag cgtgtcgatc ttccatgcaa
    14761 tggatcgcgt cggtacccgc tatgtgcaaa tcgatccgaa aaagattgtc gccgtcgtgg
    14821 aaaccaactt gcccgacgcc ggtaatatgc tggataagca aaatcccatg tgccagcaga
    14881 ttgccgataa cgtggtcacg ttcttattgc aggaaatggc gcatgggcgt attccgccgg
    14941 aatttctgcc gctgcaaagt ggcgtgggca atatcaataa tgcggtaatg gcgcgtctgg
    15001 gggaaaaccc ggaaattcct ccgttcatga tgtattcgga agtgctacag gaatcggtgg
    15061 tgcatttact ggaaaccggc aaaatcagcg gggccagcgc ctccagcctg acaatctcgg
    15121 ccgattccct gcgcaagatt tacgacaata tggattactt tgccagccgc attgtgttgc
    15181 gtccgcagga gatttccaat aacccggaaa tcatccgtcg tctgggcgtc atcgctctga
    15241 acgtcggcct ggagtttgat atttacgggc atgcaaattc aacgcacgta gccggggtcg
    15301 atctgatgaa cggcatcggc ggcagcggtg attttgaacg caacgcgtat ctgtcgatct
    15361 ttatggctcc gtcgattgct aaagaaggca agatctcaac cgtcgtgcca atgtgcagcc
    15421 atgttgatca cagcgaacac agcgtcaaag tgatcatcac cgaacaaggg atcgccgatc
    15481 tacgcggtct ttccccgctt caacgcgccc gcactatcat tgataattgt gcacatccta
    15541 tgtatcggga ttatctgcat cgctatctgg aaaatgcgcc cggcggacat attcaccacg
    15601 atcttagcca cgtcttcgac ttacaccgta atttaattgc aaccggctcg atgctgggtt
    15661 aatggtttgt cccgtcacga tcttcagcaa tatgctgaag catcgtggcg gtataacgat
    15721 gatttctgat tgaataaaga tattcctgca tatacatcgg cctgtgcggc gcatcaaaat
    15781 atttcagcgt tgccggattc accagtcgcc aggcaaaatg cgggatcacc gtcagaaaat
    15841 gtccgcgctc cacggcgctg attttggcca taaaactgta gggacgataa ataatggtgg
    15901 gattgatccc acaaggtcgc atattcgcat caatcattgc ttcgaagtta gcccggtttt
    15961 gaaagcgcat ttgtagccag ggcagctcct gaagcagctc ttgctcttga cgctcttcat
    16021 aacggcgaga aacgagaaag cccaatcgca gcggtggtaa ttcactgata gtcaggtttt
    16081 ccagttcctg aactcgtgca gatacatgct gtggggagat aataaaatct aactgacggt
    16141 caaaaagatt atcgataacg ccgttttcgc tgaactcaac tggctgggcg gtcacgcctt
    16201 catacttatc tccgagacta atcaactgat cgaaaataat tgtcggataa gtattatcta
    16261 tgccaatcac aatt
//
LOCUS       DAZPDU010000092        16250 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZPDU010000092 DAZPDU010000000
VERSION     DAZPDU010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16250
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.093"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..578
                     /locus_tag="ACHURS_002657"
     CDS             <1..578
                     /locus_tag="ACHURS_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFR1238349.1"
                     /translation="GRPNNPFEPRRNRYAQKYLTLALMGGAAFFVLKGCGDSSDVDND
                     GDGTFYATVQDCIDDGNNADICARGWNNAKTAFYADVPKNMTQQNCQSKYENCYYDNV
                     EQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASRPVWRNTSGDYSWRSGSGKKES
                     YSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            581..1744
                     /locus_tag="ACHURS_002658"
     CDS             581..1744
                     /locus_tag="ACHURS_002658"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFR1238350.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWHASDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLCKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIDSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            1828..3453
                     /locus_tag="ACHURS_002659"
     CDS             1828..3453
                     /locus_tag="ACHURS_002659"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFR1238351.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQVLCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCL"
     gene            complement(3570..3845)
                     /gene="yjfN"
                     /locus_tag="ACHURS_002660"
     CDS             complement(3570..3845)
                     /gene="yjfN"
                     /locus_tag="ACHURS_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFR1238352.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3994..4323)
                     /gene="bsmA"
                     /locus_tag="ACHURS_002661"
     CDS             complement(3994..4323)
                     /gene="bsmA"
                     /locus_tag="ACHURS_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFR1238353.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4505..5254
                     /gene="yjfP"
                     /locus_tag="ACHURS_002662"
     CDS             4505..5254
                     /gene="yjfP"
                     /locus_tag="ACHURS_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR1238354.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5251..6006)
                     /gene="ulaR"
                     /locus_tag="ACHURS_002663"
     CDS             complement(5251..6006)
                     /gene="ulaR"
                     /locus_tag="ACHURS_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFR1238355.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ERGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6114..7178)
                     /gene="ulaG"
                     /locus_tag="ACHURS_002664"
     CDS             complement(6114..7178)
                     /gene="ulaG"
                     /locus_tag="ACHURS_002664"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFR1238356.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7533..8931
                     /gene="ulaA"
                     /locus_tag="ACHURS_002665"
                     /pseudo
     CDS             7533..8931
                     /gene="ulaA"
                     /locus_tag="ACHURS_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
     gene            8947..9252
                     /gene="ulaB"
                     /locus_tag="ACHURS_002666"
     CDS             8947..9252
                     /gene="ulaB"
                     /locus_tag="ACHURS_002666"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFR1238357.1"
                     /translation="MTVRILAVCGNGQGSSMIMRMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9262..9723
                     /gene="ulaC"
                     /locus_tag="ACHURS_002667"
     CDS             9262..9723
                     /gene="ulaC"
                     /locus_tag="ACHURS_002667"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFR1238358.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVADVVEPRYYQ
                     AILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDILI
                     TMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            9737..10387
                     /gene="ulaD"
                     /locus_tag="ACHURS_002668"
     CDS             9737..10387
                     /gene="ulaD"
                     /locus_tag="ACHURS_002668"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFR1238359.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            10397..11251
                     /gene="ulaE"
                     /locus_tag="ACHURS_002669"
     CDS             10397..11251
                     /gene="ulaE"
                     /locus_tag="ACHURS_002669"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFR1238360.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARDWVKARMA
                     KAGTVEAA"
     gene            11251..11937
                     /gene="ulaF"
                     /locus_tag="ACHURS_002670"
     CDS             11251..11937
                     /gene="ulaF"
                     /locus_tag="ACHURS_002670"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFR1238361.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            12034..12585
                     /locus_tag="ACHURS_002671"
     CDS             12034..12585
                     /locus_tag="ACHURS_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFR1238362.1"
                     /translation="MAINGLFVRTDPQRRRYGVALLVGVLSGIISAFVKWGAEVPLPP
                     RTFSGGRDEFNPPYIFLRDYLGIDPTQTVYTFSEHIINPVMVTHIIFSLVFAIGYCIV
                     AEIFPKVKLWQGILAGLIVTVAVHGIFCPALNLTPPLTQLPFDEYASEILGHIFWFWI
                     IEIMRRDLRNRITHEPDPEVAIR"
     gene            complement(12660..12935)
                     /gene="yjfY"
                     /locus_tag="ACHURS_002672"
     CDS             complement(12660..12935)
                     /gene="yjfY"
                     /locus_tag="ACHURS_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFR1238363.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            13262..13657
                     /gene="rpsF"
                     /locus_tag="ACHURS_002673"
     CDS             13262..13657
                     /gene="rpsF"
                     /locus_tag="ACHURS_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFR1238364.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13664..13978
                     /gene="priB"
                     /locus_tag="ACHURS_002674"
     CDS             13664..13978
                     /gene="priB"
                     /locus_tag="ACHURS_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFR1238365.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            13983..14210
                     /gene="rpsR"
                     /locus_tag="ACHURS_002675"
     CDS             13983..14210
                     /gene="rpsR"
                     /locus_tag="ACHURS_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFR1238366.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            14252..14701
                     /gene="rplI"
                     /locus_tag="ACHURS_002676"
     CDS             14252..14701
                     /gene="rplI"
                     /locus_tag="ACHURS_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFR1238367.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14813..15784)
                     /locus_tag="ACHURS_002677"
     CDS             complement(14813..15784)
                     /locus_tag="ACHURS_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFR1238368.1"
                     /translation="MKIKVSAGIIGAVLMLSASQSWAVTLKLSHNQDKSHPVHKAMEF
                     FAKKNKEYSNGDITIRIYPNGTLGTQRETMELIRSGAIPLVKTNAAEMEAFENSYKLF
                     SLPYLFRDRDHYYQVMQGDIGRKILDSTKSKGYFGLTFYDGGARSFYGNKPVLKPDDL
                     KGMKVRVQPSPGAVEMIKVMGGNPTPLDYGELYTALQQGVVDMAENSVMALTTMRHGE
                     VAKSFSLDEHTMVPDVVLMSNAAFDKLSPENQAVILKAAKESMSYMKDLWSEEEKQEF
                     AKLDKMGVKVYQVDKAPFIEKVQPMYANFAKDNPALAPMLADIQAAK"
     gene            complement(15837..>16250)
                     /locus_tag="ACHURS_002678"
     CDS             complement(15837..>16250)
                     /locus_tag="ACHURS_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HFR1238369.1"
                     /translation="WAMSNADVPFLILDLLNTISDNPIIILLIINIILLIIGTFMDMT
                     PAVLIFTPIFLPVVTELGMDPIHFGIVMVLNMCIGICTPPVGSVLFVGCSVSKLPINK
                     IIKPMLPFYAVMVLVLAMVTYIPQISMALPKALGY"
BASE COUNT         3817 a         3968 c         4322 g         4143 t
ORIGIN      
        1 ttggtagacc gaataatcct tttgaaccgc gtcgcaatcg ctacgcacaa aaatacttaa
       61 cgttggcgct aatgggcggt gccgcttttt tcgtattgaa aggttgtggc gatagtagcg
      121 atgtcgataa cgacggcgac ggaacgtttt acgcgacagt gcaggattgt attgatgacg
      181 gtaataatgc cgacatttgc gcgcgtggct ggaacaacgc caaaacggca ttttatgccg
      241 atgttccgaa gaatatgact cagcagaact gtcagtctaa gtacgaaaat tgctactacg
      301 acaatgttga acagagttgg atcccggtcg tttctggatt tttgttaagc cgggttattc
      361 gtaaagatcg cgatgagccg ttcgtctata acagcggcgg atcctcattt gcttcccgcc
      421 ccgtctggcg caacacttcc ggtgattact cctggcgctc cggttctggc aaaaaagagt
      481 cttactcttc aggcggcttt accaccaaaa aagcgtcaac cgtttcgcgc ggcggctatg
      541 gtcgttcttc cagcgcccgt gggcattggg gaggctaatc atgctgagac acaacgttcc
      601 tgtgcggcgg gatctggacc aaattgccgc tgacaacggt ttcgactttc atatcatcga
      661 caatgaaatc tattgggacg agagtcgggc ttaccgtttt actctgcgcc agattgaaga
      721 gcagatcgaa aaaccaactg cggaactgca tcagatgtgc cttgaggtgg tggatcgcgc
      781 ggttaaagac gaagagatcc tgacgcaact ggcgatcccg ccgttgtact gggatgtgat
      841 cgccgaaagc tggcacgcca gcgatccttc gctgtatggc cggatggatt ttgcctggtg
      901 tggcaatgcg ccggtgaagc tgctggagta caacgccgat acgccgactt cgttgtatga
      961 gtcggcttat ttccagtggt tgtggctgga ggatgcccgg cgcagcggca ttattccgcg
     1021 tgatgccgat cagtacaatg ccattcagga acgcctgatt tcacgcttta gcgagcttta
     1081 tagccgggag ccgttttatt tctgctgctg tcaggacacc gatgaagaca ggactaccgt
     1141 gctgtacttg caggactgcg cccagcaggc agggcaggag tcgcggttta tctacattga
     1201 agatctcggt ttgggcgtcg gcggtgtact gaccgatctt gatgataatg tcatccagcg
     1261 tgcatttaag ctgtatccgc tggagtggat gatgcgtgac gataacggtc cgctgctgtg
     1321 caagcgtcgc gagcaatggg tggagccgtt atggaaaagt atcttgagta ataaagggct
     1381 aatgccgctg ctttggcgct tcttccctgg tcatcctaac cttcttgcat cctggtttga
     1441 gggtgaaaaa tcgcagattg ccgctggcga aagctatgtg cgtaaaccga tctactcgcg
     1501 cgaaggcgga aacgtcacca ttttcgacgg tcagaataac gtcgttgacc acgctgatgg
     1561 tgattacgcc gatgaaccga tgatctacca ggcgtttcaa cctctgccgc gatttggcga
     1621 tagctacaca ctcatcgata gctggattgt cgatgatgaa gcgtgcggaa tggggatccg
     1681 tgaagataac acactgatca ccaaagacac ctcacgtttc gttccgcatt acattgctgg
     1741 ataagaatgt tttagcaatc tctttctgtc atgaatccat ggcagtggcc atactaatgg
     1801 tgactgccat tgatggaggg agacacagtg cactggcaaa ctcacaccgt ttttaatcaa
     1861 cctataccat taaataacag caacttatac ctgtctgatg gcgcgctttg cgaagcggta
     1921 acgcgtgaag gtgctggctg ggatagcgat tttctagcca gtattggtca gcagttagga
     1981 acggctgaat cccttgaact ggggcggctg gcgaatgtaa acccgcctga actgttgcgc
     2041 tacgatgccc aggggcggcg tctggacgat gttcgtttcc atcccgcatg gcacctgctg
     2101 atgcaggtgt tatgtaccaa tcgggtgcac aatcttgcct gggaagaaga cgctcgctcc
     2161 ggcgcatttg tggcgcgcgc ggcgcgtttt atgttacatg cgcaggttga ggctgggtcg
     2221 ttatgtccaa taacgatgac ctttgccgcc acgccattgt tgttacagat gttacccacg
     2281 ccgtttcagg actggaccac gccgctgctg agcgatcgct acgattctca cttattgcca
     2341 ggtgggcaaa aacgcggttt gttgattggc atgggaatga cggaaaagca gggtggttcc
     2401 gatgtcatga gcaacaccac ccgtgcagag cgtttggaag atggctctta tcggctggtg
     2461 gggcataaat ggtttttctc ggttccgcaa agcgatgcgc atctggtgct ggcgcagacc
     2521 acgggcggtc tgtcctgttt ttttgtgccg cgctttttgc ctgacgggca acgcaacgcg
     2581 attcgcctcg aacggctgaa agataagctg ggtaatcgct ctaacgccag ttgtgaagtg
     2641 gagtttcagg atgccattgg ctggttattg gggcaggaag gggaaggaat tcgtctgatc
     2701 ctgaaaatgg gggggatgac gcgttttgat tgcgccctgg gtagccatgc catgatgcgc
     2761 cgtgcgtttt cgctggcgat ttatcatgca catcaacgcc atgtttttgg taatccattg
     2821 atccaacagc cccttatgcg tcatgtctta agtcgcatgg cacttcagct tgaagggcaa
     2881 acggcgttgc tgtttcgtct tgcgcgagcg tgggaccggc gcgccgatgc caaagaagcc
     2941 ctgtgggcgc gtttatttac gcctgcggca aaatttgtga tctgcaaacg aggtatgccg
     3001 tttgtggccg aagcgatgga ggtgctgggc ggcattggtt attgcgagga gagcgagctg
     3061 ccgcggcttt accgggagat gccggtaaac agtatttggg aaggttccgg caatattatg
     3121 tgcctggatg tgttgcgcgt tctcaataag caagcgggcg tatacgactt attgtcggaa
     3181 gcatttgtgg aagtgaaagg gcaggatcgc tattttgatc gcgcggttcg tcgtttacag
     3241 cagcagctgc gtaagccagc tgaagaactg gggcgagaga ttactcatca gctattcctg
     3301 ctgggctgcg gtgcgcaaat gttgaaatat gcttctccgc caatggcgca ggcgtggtgt
     3361 caggtgatgt tagatacgcg cggcggcgta cggttgtcag agcagatcca gaatgattta
     3421 ttgctgcggg cgacgggggg agtgtgtttg taaacgtata cgactgatgc gacgctggtt
     3481 tcgattaact caatgaaata tgtgaaaatt gtaggccgga caaggcgctc gcgccgcatc
     3541 cggcattgtt catttgctgt agatgacact catgcataca gtatcgcctg tacgcgccag
     3601 ttatcaggct gctggtcttc gtacatggta ataatgcggt accatgacgc accttgttca
     3661 tcggccttta acgccacgat acgttccacg tcctgcggat cccctgaaat attgctaact
     3721 gagatctgac ctatttcatt caggcccgtc acgcagtcag cactggcgaa ttctgcagac
     3781 tgtgccgtgg cgctcgcagg ataagtcact aattgcagcg agggtgaggc aagtaattgt
     3841 ttcatcgtca gctccatttt tcgtttcccc acatgttgca gggcattcca tcgcggtaat
     3901 aaacgcccag gcttccagcc ggtaatttat tgtgcacagg taaacgcata gggacgcaaa
     3961 gcaatgtaaa gctgactatg taagttgtct gatttattta cggtacaaaa tggcttgtga
     4021 ataccactgt cctgtcacga tggtttcgtc caccatgacg acaacgtaat aatcagcttt
     4081 tgcagcgaca gcttttgctc tgatttctgc taatgcgtca tccggagaac cccgaaccat
     4141 cgtgcttacg ctacctattc gctgtaaccc ttgcgtctgg tcgcggcgaa tctcttgcgg
     4201 atggtccgtt actggcggtg ctggctgtgg cgtaccttgc agtgcgctac aggcacttaa
     4261 catcaacacc aataataaac tggcaaaccg gtaaataacg ctattacgtt tcctgctaac
     4321 catagtgtag tgccttatta atttaacttt gggggaatgt tcccgaattg ttacctggta
     4381 cgcgattttc gaatgaaaac gtgacgaaag cgtaacccac atctcaacat tatgaactaa
     4441 taccgccagc agaaacttgt gctaaggatt tcttcatgtc ataccagaca aaaaggagag
     4501 acagatgatt gaaatagaat cacgcgagct ggcagatatt cccgttcttc atgcttatcc
     4561 tgtcgggcaa aaagataccc cgttaccgtg cgtaattttt tatcacggct ttacttcatc
     4621 cagcctggtg tatagctatt ttgccgttgc gctggcgcag gctggtttgc gggtgatcat
     4681 gccggatgcg cccgatcacg gtagccgttt tagtggcgac gaagcgcggc ggttaaatca
     4741 attctggcaa atcttgctac aaagtatgca ggaattcact actttacgtg cggcaatagc
     4801 cgaagaaaac tggctgcttg atgaccgtct ggcagtcggt ggcgcgtcga tgggcgcgat
     4861 gacggcactg ggaattaccg ctcgccaccc cacggtgaaa tgtaccgcca gcatgatggg
     4921 atcgggctat tttacatcgc tcgcccgttc actgtttcca ccgctgatac ctgaaacggc
     4981 agcacagcag aatgaattca ataacattat cgcgccgctg gcagagtggg aagcgacaaa
     5041 ccacctggaa caacttgggg acagacctct actgctgtgg catggcctcg acgatgacgt
     5101 tgtgcctgcc gacgaatcac tacgtttgca gcaggcctta agcgagacgg gacgggataa
     5161 actgctaacc tgttcatggc agccaggcgt gcgtcaccgc attacgcctg aggcgttaga
     5221 tgctgccgtg acatttttcc gccagcatct ttaaacacgc agaatgctga ccccttgcgc
     5281 ttccagttgt tgcaggattt ccgggttagc atttttgccg gtgataagca tatcgatttg
     5341 atcggcacgg ctaaaaagca ttcccgcgcg ttcgccaatc ttactgctat caaccagtac
     5401 caccagtttc cctaccacgc tcagcatctt ctgctctgcc attgctgtca gcatatcggt
     5461 tttatacaac ccttctgcgg tcagcccttt tccgctggta aacatccagt gcccggcata
     5521 gagactgttt tcgctgccct gcgggcttaa agtgatggac tgacttttgt tgtactgtcc
     5581 acccataatg atcacgctgt catgttcttg atcgatcagg taatttgcca gcggtagata
     5641 attagtgatg atttgcactg gcttgccaca catttcccgc ccaagcagaa acgcggtgga
     5701 gccgcagttg atgactacgc tttcgcccgg attaaccagc tgcgaggccg ctttagcaat
     5761 acgtactttt tcatcgtgat tctgcgcctg atgcagattc atcggcgtcc agcgcgggcg
     5821 ctgttgggta atagcttctg cgccattgcg cacctttttc agtttgccgc gttcgtcaag
     5881 tttattgata tcgcgtcgcg cagtggcagg cgaaattccc agacgctcaa cgactttctc
     5941 aacggtcaca aagcccaatt gtgcgagcat ttccaggagg atttgatgtc tttgtgcttc
     6001 agtcatgagc tattccgata aaatttgatt tttttagatg atatttgaaa tagctaggaa
     6061 atactacgcc ggatagcgcg aacttctcca cgctatccgg tataaacgac agattacagg
     6121 aatgacttga acggcagatc cggttcaata gtgaagcaat catcgaaacc gcgcggatag
     6181 tggtactcga agttgtcttt atccagcggc caggtaaatt tgccgccaac ctgccagata
     6241 aacggcttga agccatactt cagacgatct tttttcatct cccacagcac gcggatctct
     6301 tgcggatcgg cctggaagtt tgaccagatg tcgtggtgga acgggataac cactttcgca
     6361 ttcagggctt cacccatacg cagcatatcg gcgctggtca ttttgtcggt aataccgcgc
     6421 gggttctcac catacgatcc taacgccaca tcgatctgat gttcgttacc gtgcttcgca
     6481 taatagttag agtagtggga atcgccactg tgatataggg aaccgccagg ggttttgaac
     6541 aggtagttta ctgcgcgatc gtccatgcca tctggcagta cgccagccgc tttttgatcg
     6601 gcaggcaggg tgatcagtgc agtacggtcg aaagcgtcaa gcgcatgaat ttcaatgtct
     6661 ttcactttta caacatcgcc cggtttgacc acgatgcaac gctctttcgg tacgccccag
     6721 ccaatccaca aatcaacgca ggttttcggt ccaatgaatg gtacgtcatc agcacagttc
     6781 tgcatcacgg cagcagcgac gttaacgtcg atatgatcgt tgtgatcgtg agtcgccagt
     6841 accgcgtcga tctggcgaat cgcaaacgga tcaagaacaa acggggtggt gcgtaggttt
     6901 ggctgcagtt ttttcacgcc agccatgcgc tgcatctggt gaccctgttt cattaacggg
     6961 ttaccgtgac tttgtttgcc agtgccgcac cagaaatcaa cgcaaacgtt ggtgccacct
     7021 tctgatttca accagatccc ggtgcagcct agccaccaca tcgcaaatgt gccaggggcg
     7081 acctgctctt gttcaatttc ttcattcagc cagctacccc actccgggaa agtgctcagg
     7141 atccaggatt ctcgggtgat acttttcact ttactcatcg ccatttacct tcatgatagt
     7201 tcaattcgaa tcaatatgtg attggttttg attaatcctg acactatttt ttcaggaagg
     7261 caatgaccat tttttgactt tttgccaggg aagttgttgt tgattttgag tgtggaaaga
     7321 tttaatggaa tgtgtagtta aattaactaa atgaatttaa ataaataatt gtttcgttgt
     7381 gtgaatccca ctctatccgt gtggaattat ttgcgggtcg cgtcacattt aatcataaat
     7441 aatcttgttg tgattacttt tgaaaattag agtgagtgca caacattccg ggtgtgcgga
     7501 atacccggtt acctcttctt caggagatcg ttatggagat cctctacaac atctttaccg
     7561 tgttttttaa ccaggtcatg accaatgccc cgttgttgct gggtattgtg acctgtctgg
     7621 gctacatcct actgcgcaaa agtgtcagcg ttattattaa aggcacgatt aaaaccataa
     7681 ttggtttcat gttgttgcag gcagggtccg gcatcctcac cagcaccttc aaaccggtgg
     7741 tggcgaaaat gtccgaagtc tacggcatta aacggcgcaa tttccgatac ttacgcgtcg
     7801 atgatggcaa ccatcgaccg catgggcgat gcctatagct gggtaggtta cgcggtgttg
     7861 ttagcgctgg cgctgaacat ctgttacgtg ctgctgcgtc gcattaccgg tattcgtacc
     7921 atcatgctga ctggtcatat catgttccag caggcggggc tgattgccgt tacgttgttt
     7981 attttcggct actccatgtg gacaaccatt atctgcacgg cgattctggt ttcgctctac
     8041 tggggcatta cctccaacat gatgtacaag ccgactcagg aagtgacgga cggctgcggt
     8101 ttctccatcg gtcaccagca acagtttgca tcatggactg cctataaagt tgcgccgttc
     8161 ctcggcaaaa aagaggaaag cgttgaagac ctcaaactgc caggctggct gaatattttc
     8221 cacgacaaca tcgtctccac ggcgattgtc atgaccatct tctttggtgc cattctgctc
     8281 tccttcggta tcgacaccgt gcaggcgatg gcaggcaaag tgcactggac ggtctatatc
     8341 ctgcaaaccg gtttttcctt cgcggtggcg atcttcatca tcactcaggg tgtgcgcatg
     8401 tttgtggcgg aactctctga agcgtttaac ggtatctccc agcgcctgat ccctggtgcg
     8461 gttctggcga ttgactgtgc ggctatttat agcttcgcgc cgaacgccgt ggtctggggc
     8521 tttatgtggg gcaccatcgg tcagctgatt gcggttggca tcctggtcgc ctgcggttcc
     8581 tcgatcctga ttatcccagg ctttatcccg atgttcttct ccaacgccac catcggcgtg
     8641 ttcgctaacc acttcggcgg ctggcgtgcg gcgctgaaga tttgtctggt gatggggatg
     8701 atcgaaatct ttggttgcgt ctgggcggtg aaacttaccg gtatgagtgc ctggatgggg
     8761 atggcggact ggtcgattct ggcaccgccg atgatgcagg gcttcttctc catcggtatc
     8821 gcctttatgg ccgtcatcat tgtaattgca ctggcttata tgttcttcgc tggccgcgca
     8881 ctgcgcgcag aagaagatgc agaaaaacaa ctggcagaac agtctgctta ataaggagtt
     8941 ttgattatga ccgtacgtat tctggctgtg tgtggcaacg gacaaggcag ttccatgatc
     9001 atgaggatga aagtggacca gtttttaacc caatcaaaca ttgaccacac ggtaaatagc
     9061 tgcgcggttg gcgaatacaa aagcgagttg agcggcgcgg atatcatcat cgcttctacg
     9121 cacattgcgg gcgaaatcac cgtgaccggc aacaaatacg tggttggcgt gcgcaacatg
     9181 ctctctcctg ccgactttgg cccgaaactg ctggaagtga tcaaagcgca tttcccgcag
     9241 gatgtgaagt aaggacgcgc catgaaatta cgtgattcgc tggcggagaa taaatccatc
     9301 cgcctgcagg ctgaagctga gacatggcag gacgcagtaa aaatcggcgt tgacctgctg
     9361 gtggcggatg ttgtcgagcc gcgttactac caggcgattc tggatgcggt tgaacagcac
     9421 ggtccgtact ttgtgctcgc accaggcctg gcaatgccgc acggtcgccc ggaagagggc
     9481 gttaagaaaa ccggttttgc gctggtgacg ctgaaaaagc cgctggagtt caaccacgaa
     9541 gataacgacc cggtggatat cctcatcacc atggcggcgg tcgatgccaa cacgcaccag
     9601 gaagtgggca tcatgcagat cgtcaacctg tttgaagatg aagaaaattt cgaccgttta
     9661 cgtgcctgcc gtaccgagca ggaagtactg gatctcattg accgcaccaa cgcggcagct
     9721 taagaaggaa ttgaagatgt cattaccgat gttgcaagtc gcgctggaca accagactat
     9781 ggatagcgcc tacgaaacca ctcgcctgat tgccgaagaa gtcgacatta tcgaagtggg
     9841 taccattctg tgcgtgggcg aaggcgtgcg tgcggttcgc gatctgaaag cgctctaccc
     9901 gcacaaaatc gtgctggcag acgccaaaat tgccgatgcg ggcaaaattc tttctcgtat
     9961 gtgcttcgaa gccaacgctg actgggtgac cgtgatttgt tgtgcggata tcaacaccgc
    10021 caaaggtgcg ctggacgtag caaaagagtt taacggcgac gtgcagatcg aactgaccgg
    10081 ttactggacc tgggaacagg cgcaacagtg gcgcgacgca ggtattcagc aggtggttta
    10141 tcaccgcagc cgtgacgcgc aggccgctgg cgtggcgtgg ggtgaagccg atatcactgc
    10201 gatcaaacgt ctttccgata tgggcttcaa agtcaccgtc accggtggcc tggcgctgga
    10261 agatctgccg ctgttcaaag gcattccaat tcatgtgttt atcgccggtc gcagcatccg
    10321 tgatgccgct tctccggttg aagccgcgcg tcagttcaaa cgttccatcg ccgaactgtg
    10381 gggctaagga gcggatatgt tgtccaaaca aatcccgctt ggcatctatg aaaaagcgct
    10441 ccccgccggg gagtgctggc tggaacgcct gcgactggca aaaacgttgg gcttcgattt
    10501 tgtcgagatg tcggtagatg aaactgacga gcgcctgtcg cgcctcgact ggagccgcga
    10561 gcagcgtctg gcgctggtga atgcgattgt tgaaaccggc gtgcgtgtgc cgtccatgtg
    10621 cctttctgcc catcgtcgtt tcccgttggg cagcgaagat gacgcggtgc gggcgcaggg
    10681 gctggagatt atgcgtaaag ccatccagtt cgcccaggac gttggcattc gcgtgatcca
    10741 gctggcgggc tatgacgttt actatcagga agccaataac gaaacgcgtc gtcgtttccg
    10801 tgacggcctg aaagagagcg ttgagatggc gagccgcgcg caggtgacgc tggcgatgga
    10861 gatcatggat tatccgttga tgaactccat cagcaaggcg ctgggttacg cgcactatct
    10921 caacaacccg tggttccagc tctacccgga tatcggcaac ctgtcggcgt gggacaacga
    10981 tgtgcagatg gagttgcagg ccggaatcgg gcatatcgtc gcggtgcatg tgaaagacac
    11041 caaaccgggc gtcttcaaaa acgtgccatt tggcgaaggt gtggtggatt tcgaacgttg
    11101 cttcgaaacg ctcaaacaga gtggctattg cgggccgtac ctgattgaga tgtggagcga
    11161 aacggcggaa gacccggcgg cagaagtggt gaaagcgcgt gattgggtga aagcgcgcat
    11221 ggccaaagca ggcacggtgg aggcggcata atgcaaaagc taaaacagca ggtgtttgaa
    11281 gccaacatgg atctgccgcg ctatgggctg gtgaccttta cctggggcaa cgtcagcgct
    11341 atcgaccgcg aacgcgggct ggtggtgatc aagcccagcg gcgttgccta cgaaaccatg
    11401 aaagcagacg atatggtggt ggtcgatatg agcggcaagg tggtggaagg ggagtatcgc
    11461 ccgtcttccg acaccgcgac gcatctcgaa ctctaccgtc gttacccgtc gcttggcggc
    11521 attgtccata cccactccac tcatgctact gcatgggcgc aggcggggct ggcgatcccg
    11581 gcgttaggca ctacgcacgc cgactacttc tttggcgaca tcccgtgtac gcgcggatta
    11641 agcgaagaag aggtgcaggg cgagtatgaa ctgaacaccg gcaaagtgat tatcgaaacg
    11701 ctgggtgacg ccgagccgct gcatacgccg gggattgtgg tgtatcagca cggtccgttc
    11761 gcctggggga aagatgccca cgacgcggtg cataacgcgg tggtgatgga agaagtggcg
    11821 aaaatggcgt ggattgcgcg cagtattaac ccacaactga atcacatcga cagcttcctc
    11881 atgaataaac acttcatgcg taagcacggt cctaacgcct attacgggca gaagtaagca
    11941 gtgcgccccg gaattattac ctcctgcggg atgtaattgg tctgattccg gggccgcttc
    12001 agttaaaaaa tcgtataaac aacaggtgtt tttatggcta tcaatggatt gtttgtcaga
    12061 acggatccgc aacgtcgccg ctatggcgtt gcgttacttg tgggtgtact ttcaggaatt
    12121 atttcagcat ttgttaaatg gggagcggaa gtccccttgc caccgcgcac attttccggt
    12181 ggtcgcgatg aatttaaccc gccgtatatc tttctacggg attatttagg tatcgaccca
    12241 acccaaactg tgtatacatt ctcggaacat attatcaacc cggtgatggt gacgcatatc
    12301 atcttctcac tggtttttgc gatagggtat tgtattgtcg cggaaatttt cccgaaagtg
    12361 aaattatggc aggggatatt agcagggctt attgtgacag tggcagtcca cggtatattt
    12421 tgcccggcgc tgaatttaac gccgccgctc actcaattgc cttttgacga atatgcctct
    12481 gaaatcctgg ggcatatttt ctggttctgg ataatagaaa ttatgcgccg cgacttacgt
    12541 aatcgcatta ctcatgagcc agatccggaa gtcgcaatcc ggtgaataac cgcataaaaa
    12601 agccccctgc gaagggggca aagcaaacta tggcaatgtt tcgttggtta tacctggtgc
    12661 tagcgataaa tatccgcgct ggcgtgcata ttgccgttac tccccggttc ccgcatcaga
    12721 attacgtggt agtacgttgc gccctgcgca tctgtctctt catttaatgc ctgacgcgct
    12781 tcgctttcag tggcgaaatt atgattgata taaatcacgc ctaagctttg cacatcatcc
    12841 atgtttctgg cctggtggtt attaatttca atggctgccc atgtatttgc acttagcaaa
    12901 agcacagcca gaagggctaa aactcgactg aacatagata cctcctcgac ggctgacttt
    12961 gtgtgctctc ctctgtgatg atcttctgat ttaattttaa tcaatgataa agaagttgat
    13021 ggtgaccatt tctgatgcag ttgttcaaaa aaacaccatg atgaagtgtg atgaactcca
    13081 aatcagcggt ttagaggtta attgcgaaag gggagattta tttcggctct gcccttgagt
    13141 ttagcggggc atacaagtac tataacgcgt catttttcag ccgaccttta acacgttcct
    13201 tgcctccccg ggattcggct gacccagaca ggaggctgaa taatccgtaa ggagcaattc
    13261 gatgcgtcat tacgaaatcg tttttatggt ccatcctgac cagagcgaac aggttccggg
    13321 catgatcgag cgctacactg ctgccatcac tggtgcagaa ggcaagatcc accgtctgga
    13381 agactggggc cgccgtcagc tggcttaccc gatcaacaaa ctgcacaaag cacactacgt
    13441 tctgatgaac gttgaagctc cgcaggaagt gatcgatgag ctggaaacta ccttccgctt
    13501 caacgatgcc gttatccgca gcatggttat gcgtaccaag cacgctgtta ccgaagcatc
    13561 tccgatggtt aaagcgaaag acgagcgccg tgagcgtcgc gatgatttcg caaacgaaac
    13621 cgctgatgat gctgaagctg gggattctga agagtaattt ctgatgacca accgtctggt
    13681 gttgtccggc accgtgtgca ggactcccct tcgaaaggtc agtccatcag gaattcctca
    13741 ctgccagttc gtgcttgagc atcgttctgt gcaggaggaa gccggttttc accggcaggc
    13801 gtggtgccaa atgcccgtta ttgttagcgg acacgaaaac caggccatta ctcacagtat
    13861 aacggtcggc agtcgcataa ccgttcaggg gttcatttca tgccacaagg caaagaacgg
    13921 actgagcaaa atggttttgc atgccgagca gattgaattg atagattctg gagactagcc
    13981 atatggcacg ttatttccgt cgtcgcaagt tctgccgttt caccgcggaa ggcgttcaag
    14041 agatcgacta taaagatatc gctacgctga aaaactacat caccgaaagc ggtaagattg
    14101 tcccaagccg tatcaccggt acccgtgcaa aataccagcg tcagctggct cgcgctatca
    14161 aacgcgctcg ctacctgtcc ctgctgccgt acactgatcg ccatcagtaa tcggtcacgg
    14221 tccattaata cgactttgag aggataaggt aatgcaagtt attctgcttg ataaagtagc
    14281 aaacctgggt agcctgggtg atcaggtaaa cgttaaagcg ggctatgctc gtaacttcct
    14341 ggtaccgcag ggtaaagctg ttccagctac caagaaaaac attgaattct tcgaagcacg
    14401 tcgcgctgaa ctggaagcta aactggctga agttctggca gctgctaatg ctcgcgctga
    14461 gaaaatcaat gcactggaaa ctgttaccat cgcgtctaaa gctggcgacg aaggtaaact
    14521 gttcggttcc atcggtactc gcgacatcgc tgacgctgta actgcagctg gcgttgaagt
    14581 ggctaagagc gaagttcgtc tgccgaacgg cgttctgcgt accactggcg agcacgaagt
    14641 gagcttccag gttcacagcg aagtattcgc gaaagtgatc gtaaacgtag tagctgaata
    14701 attcgttatt caacgagacg taaaaacgcc gaccagtggt cggcgttttg cttttgtagc
    14761 gagcacttgc agatatcgat aagggccaca ataagcagcc ctacgaagat aattacttag
    14821 ctgcctgaat atcagccagc attggggcaa gggctgggtt gtccttagcg aagtttgcgt
    14881 acattggctg tactttctcg ataaacggag ctttatctac ctggtagact ttcacgccca
    14941 ttttatccag ttttgcaaat tcttgtttct cttcctcgct ccacaagtct ttcatgtagc
    15001 tcattgattc tttagctgct tttaatataa ctgcctgatt ttccgggcta agtttatcaa
    15061 acgcagcatt gctcatcaga accacatcgg gaaccatagt gtgttcgtca aggctgaagg
    15121 attttgccac ttcaccgtga cgcatggtgg tcagcgccat cacgctgttt tctgccatat
    15181 cgaccacacc ctgctgtaag gctgtataca actcgccgta atccagtggc gtcgggttac
    15241 cgcccatgac tttgatcatt tcaactgcgc cagggcttgg ctggacacgg actttcatgc
    15301 ctttcagatc gtctggtttc agtactggtt tattgccata gaaactgcgg gcgcctccat
    15361 cataaaaagt cagcccgaaa taacctttgc ttttcgttga gtcgaggatt tttctcccga
    15421 tatcgccctg catgacctga taataatgat cacgatcgcg gaacaaataa ggcaggctaa
    15481 ataatttata ggaattttca aatgcttcca tttctgccgc attggttttt accagtggaa
    15541 tagcgccaga acgaatcagc tccattgttt ctcgttgagt acccaatgtt ccatttggat
    15601 aaatacgaat agtaatatca ccgttagagt actctttgtt ctttttcgca aagaactcca
    15661 tcgctttatg aacaggatga gacttatcct gattatgact cagttttaat gtcactgccc
    15721 aggactggct tgcggataac ataagaacag caccgataat gccagcggat acttttattt
    15781 tcatagatgt tccttttcta ataaatatgg cggcaggtgc cgccagtgaa tgcgcatcag
    15841 tagcccagtg cttttggcaa tgccatactg atttgcggaa tataggtcac cattgccagc
    15901 accaatacca tcacggcata gaatggcagc ataggtttga taattttatt aatcggtaat
    15961 ttagatacac tacaaccgac aaataacaca ctgcctacag gcggagtaca aataccgata
    16021 cacatgttca gtaccatgac aataccaaag tgaatcgggt ccatgcccag ttcggtgaca
    16081 acaggtaaaa atatcggcgt aaaaatcagt accgccggtg tcatatccat aaaagtgccg
    16141 ataatcaaca ggataatatt gataataagc aggattatta ttgggttgtc agaaatagtg
    16201 ttaagcaaat cgagaatcag gaagggaacg tcagcgtttg acattgccca
//
LOCUS       DAZPDU010000093        16048 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZPDU010000093 DAZPDU010000000
VERSION     DAZPDU010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16048
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.094"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(36..1859)
                     /gene="typA"
                     /locus_tag="ACHURS_002679"
     CDS             complement(36..1859)
                     /gene="typA"
                     /locus_tag="ACHURS_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFR1238370.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            2232..3641
                     /gene="glnA"
                     /locus_tag="ACHURS_002680"
     CDS             2232..3641
                     /gene="glnA"
                     /locus_tag="ACHURS_002680"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFR1238371.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            3815..4864
                     /gene="glnL"
                     /locus_tag="ACHURS_002681"
     CDS             3815..4864
                     /gene="glnL"
                     /locus_tag="ACHURS_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFR1238372.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            4876..6285
                     /gene="glnG"
                     /locus_tag="ACHURS_002682"
     CDS             4876..6285
                     /gene="glnG"
                     /locus_tag="ACHURS_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFR1238373.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            6397..6507
                     /locus_tag="ACHURS_002683"
     CDS             6397..6507
                     /locus_tag="ACHURS_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFR1238374.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(6697..8070)
                     /gene="hemN"
                     /locus_tag="ACHURS_002684"
     CDS             complement(6697..8070)
                     /gene="hemN"
                     /locus_tag="ACHURS_002684"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFR1238375.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(8259..8768)
                     /gene="yihI"
                     /locus_tag="ACHURS_002685"
     CDS             complement(8259..8768)
                     /gene="yihI"
                     /locus_tag="ACHURS_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFR1238376.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVAEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(8887..9053)
                     /locus_tag="ACHURS_002686"
                     /pseudo
     CDS             complement(8887..9053)
                     /locus_tag="ACHURS_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9350..9982
                     /gene="yihA"
                     /locus_tag="ACHURS_002687"
     CDS             9350..9982
                     /gene="yihA"
                     /locus_tag="ACHURS_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFR1238377.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(10364..13150)
                     /gene="polA"
                     /locus_tag="ACHURS_002688"
     CDS             complement(10364..13150)
                     /gene="polA"
                     /locus_tag="ACHURS_002688"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFR1238378.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            13514..14446
                     /locus_tag="ACHURS_002689"
     CDS             13514..14446
                     /locus_tag="ACHURS_002689"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFR1238379.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(14487..15917)
                     /locus_tag="ACHURS_002690"
     CDS             complement(14487..15917)
                     /locus_tag="ACHURS_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFR1238380.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAVHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKLLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT         3803 a         4097 c         3942 g         4206 t
ORIGIN      
        1 agcgatggca ggttttttat aactaaaaaa cgaaattaat cgtctttcgg tgcgcggttg
       61 gcgcgacgac gatcgttttc cgtcaggtga cgtttacgaa tacggataga ggtcggagtc
      121 acttctacca gttcgtcatc atcgatgaac tccagagctt gttccagagt catgcggata
      181 ggcggaacca gaacaacagc ttcgtcagta ccggaagcac gcatgttggt cagtttctta
      241 ccggtcaggc agtttacagt caggtcgtta gagcggctgt gaataccgat aatctgacct
      301 tcgtaaactt ctgcaccgtg accgaggaac agcttaccgc ggtcctgcag accgaacagt
      361 gcgaacgcga ccgctttacc ctgaccgtta gagatcagta cgccgttctg acgctgaccc
      421 acttcacccg gacgtacgtc gtcgtagtgg ctgaaggtgg agtacagcag accagtaccg
      481 gaagtcatgg tcatgaactc agaacggaag ccaatcagac cacggcttgg gatcacgtag
      541 tcgagacgta cgcggccttt accgtctgga ttcatgtttt tcaggtcgcc tttacgttcg
      601 cccagcgcct gcattacaga accctgatgc tgttcttcaa cgtccagagt cacgttttca
      661 tacggctctt gtttacgacc gtcgatttca cggaagatta cttttggacg ggataccgcc
      721 agttcgaaac cttcacgacg catgttttcg atcagaacag acaggtgcag ttcgccacga
      781 ccagaaacgc ggaacgcatc ggcgtcttcg gtttcttcta cgcgcagcgc aacgttgtgt
      841 accagttctt tgttcagacg atccaggatc tgacgagacg ttacgaactt accttcttta
      901 ccgcagaacg gcgaggtgtt aacgcagaag aacatagaaa cggtcggctc atcaacggag
      961 agtgccggca gcgcttcaac gttttgcgtg tcgcaaacgg tgtcagaaat gttcagttcg
     1021 ccaaggcccg tgatcgccac gatatcgcca gcttccgcca gatcggtttc gatacgttcc
     1081 agaccgaggt ggcccagcac tttaccgact ttcgcgttgc gggttttgcc ttcgctatcg
     1141 atgatagtga cctgctggtt cggcttcact ttaccgcgct tgatgcggcc aatgccgata
     1201 acgccaacat agctgttgta atcgagctga gaaatctgca tctggaacgg accgtcaagg
     1261 tcaacgtccg gcgcaggaac gtggtcaaca atcgcctggt acagcggggt catgtcttcc
     1321 gccatatctt cgtggtccag acccgcgata ccgttcagcg cagaagcgta aacgatcggg
     1381 aagtccagct gctcgtcggt cgcgtcgagg ttaacgaaca gatcgaatac ctgatccaca
     1441 acccaatcag gacgcgcgcc agggcggtca actttgttga taacaacaat cggcttcagg
     1501 ccgtaagcaa acgctttttt ggttacgaag cgcgtttgcg gcatcgggcc gtcaaatgcg
     1561 tcaaccacca gcagcactga gtctaccatg gacattacac gttcaacttc accaccgaag
     1621 tcggcgtgcc ccggggtatc aacgatgttg atacggtaat cattccattt gatagcggtg
     1681 tttttcgcga ggatggtaat cccacgctct ttctccaaat cgttggagtc catcacgcgc
     1741 tcttgggttt cggcacgaga gtcgaacgta ccggattgtt ggagcagctt gtctaccagg
     1801 gtggttttac catggtctac gtgcgcgatg atggcgatat tacgcaattt ttcgatcaca
     1861 actttgcctc aggcattaga aatagcgcgt tattgtacac ggattaatcg cactacaaaa
     1921 caggatcaca aacatcctcc gcaaacaagt attgcagagt ccctttgtga tcgctttcac
     1981 ggagcataaa aagggttatc caaaggtcat tgcaccaaca tggtgcttaa tgtttccatt
     2041 gaagcactat attggtgcaa cattcacatc gtggtgcagc ccttttgcac gatggtgcgc
     2101 atgataacgc cttttagggg caatttaaaa gttggcacag atttcgcttt atctttttta
     2161 cggcgacacg gccaaaataa ttgcagattt cgttaccacg acgaccatga ccaatccagg
     2221 agagttaaag tatgtccgct gaacacgtac tgacgatgct gaacgagcac gaagtgaagt
     2281 ttgttgattt gcgcttcacc gatactaaag gtaaagaaca gcacgtcact atccctgctc
     2341 atcaggtgaa tgctgaattc ttcgaagaag gcaaaatgtt tgacggctcc tcgattggcg
     2401 gctggaaagg cattaacgag tccgacatgg tgctgatgcc agacgcatcc accgcagtga
     2461 ttgacccgtt cttcgccgac tccaccctga ttatccgttg cgacatcctt gaacctggca
     2521 ccctgcaagg ctatgaccgt gacccgcgct ccattgcgaa gcgcgccgaa gattacctgc
     2581 gttccactgg cattgccgac accgtactgt tcgggccaga acctgaattc ttcctgttcg
     2641 atgacatccg tttcggatca tctatctccg gttcccacgt tgctatcgac gatatcgaag
     2701 gcgcatggaa ctcctccacc caatacgaag gtggtaacaa aggtcaccgt ccggcagtga
     2761 aaggcggtta cttcccggtt ccaccggtag actcggctca ggatattcgt tctgaaatgt
     2821 gtctggtgat ggaacagatg ggtctggtgg ttgaagccca tcaccacgaa gtagcgactg
     2881 ctggtcagaa cgaagtggct acccgcttca ataccatgac caaaaaagct gacgaaattc
     2941 agatctacaa atatgttgtg cacaacgtag cgcaccgctt cggtaaaacc gcgaccttta
     3001 tgccaaaacc gatgttcggt gataacggct ccggtatgca ctgccacatg tctctgtcta
     3061 aaaacggcgt taacctgttc gcaggcgaca aatacgcagg tctgtctgag caggcgctgt
     3121 actacattgg cggcgtaatc aaacacgcta aagcgattaa cgccctggca aacccgacca
     3181 ccaactctta taagcgtctg gtcccgggct atgaagcacc ggtaatgctg gcttactctg
     3241 cgcgtaaccg ttctgcgtct atccgtattc cggtggtttc ttctccgaaa gcacgtcgta
     3301 tcgaagtacg tttcccggat ccggcagcta acccgtacct gtgctttgct gccctgctga
     3361 tggccggtct tgatggtatc aagaacaaga tccatccggg cgaagccatg gacaaaaacc
     3421 tgtatgacct gccgccagaa gaagcgaaag agatcccaca ggttgcaggc tctctggaag
     3481 aagcactgaa cgaactggat ctggaccgcg agttcctgaa agccggtggc gtgttcactg
     3541 acgaagcaat tgatgcgtac atcgctctac gtcgcgaaga agatgaccgc gtgcgtatga
     3601 ctccgcatcc ggtagagttt gagctgtact acagcgtcta atagttgaag ttgtactacc
     3661 cggcgcaaca acgccgggat ttagttgccg tggaaacttt cagcccatct ctgcatgggc
     3721 ttttttctcc gtcaattctc tgatgcttcg cgctttttat ccgtaaaaag ctataatgca
     3781 ctaaaatggt gcaacctgtt caggagactg ctttatggca acaggcacgc agcccgatgc
     3841 tgggcagatc ctcaactcgc tgattaacag tattttgtta attgatgaca atctggcgat
     3901 ccattacgcc aaccccgccg cgcaacaact gctcgcccaa agctcccgca aattgtttgg
     3961 tacaccgtta ccggaactgt tgagctactt ctcattaaat atcgagctga tgcaagaaag
     4021 tctggaggcg gggcaaggtt ttaccgataa cgaagtgacg ctggtcatcg acgggcgctc
     4081 gcatatcctt tctgtgacgg cccagcgtat gccggacggc atgatcctgc tggagatggc
     4141 tccgatggat aaccagcgcc gcttaagtca ggaacagcta cagcacgccc agcaggttgc
     4201 tgcccgtgat ttagtgcgcg gcctggcaca tgagattaaa aatccgcttg gaggtttacg
     4261 tggcgcggcg cagctgctca gcaaagcgtt acctgaccca tcactactcg aatataccaa
     4321 agtgattatc gaacaggcgg accggctgcg aaatctggtc gaccgtctgt tggggccgca
     4381 gctgcccggt acgcgcgtta ccgaaagtat tcacaaagta gctgaacgcg tggtgacgct
     4441 ggtgtcgatg gaactgccgg acaacgtgcg gttgattcgt gattacgacc ccagcctgcc
     4501 ggaactggcg cacgacccgg atcaaattga acaggtcttg ctgaatattg tgcgcaatgc
     4561 gctacaggcg ctggggccgg aaggcggtga aatcattctg cgtacccgca ccgcgtttca
     4621 actgacctta cacggcgagc gctaccggct ggcggcgcgg attgatgtgg aagataacgg
     4681 gccgggcatt ccgcctcatt tgcaggatac gctgttttac ccgatggtca gcggccgcga
     4741 aggtggcacc gggcttggct tatccatcgc ccgtaatttg attgatcagc attcaggcaa
     4801 aattgaattt accagttggc caggtcatac cgagttctcg gtttacctgc ctatcaggaa
     4861 ataaaggtga cgtttatgca acgagggata gtctgggtag tcgatgacga tagttccatc
     4921 cgttgggtgc ttgaacgtgc gctcgctgga gcgggtttaa cctgtacgac atttgagaac
     4981 ggcgcggaag tactggaggc gctggcgagc aaaacgccgg atgtgctgct ttcagatatc
     5041 cgtatgccgg gaatggacgg gctggcgctg ctcaagcaga ttaaacagcg ccatccaatg
     5101 cttccggtca tcattatgac cgcacattcc gatctggatg ctgccgtcag cgcctatcaa
     5161 caaggggcgt ttgattatct gcccaaaccg tttgatatcg acgaagcagt ggcgctggtt
     5221 gagcgcgcta tcagtcatta ccaggaacag cagcagccgc gtaatattca gcttaacggc
     5281 ccaacgaccg atatcatcgg cgaagcgcca gccatgcagg acgtgttccg tattatcggt
     5341 cggctttcgc gttcttctat tagcgtgctg attaacggcg aatccggcac cggtaaagaa
     5401 ctggtcgctc atgccctgca tcgccacagt ccgcgcgcca aagcgccgtt tatcgcgctg
     5461 aatatggcag ctatcccaaa agatttgatc gaatcagaac tgtttggcca cgagaaaggc
     5521 gcgtttactg gcgcgaatac cattcgtcag gggcgttttg aacaggccga tggcggtaca
     5581 ttattcctcg acgaaattgg tgatatgccg ctggatgtgc agacgcgttt gctgcgcgtg
     5641 ctggcagacg gtcagtttta ccgcgttggc ggctatgcgc cggtgaaagt ggatgtgcgt
     5701 attatcgctg ccactcacca gaatctcgaa caacgagtgc aggaaggtaa gttccgtgag
     5761 gatctgttcc accgcctgaa cgttatccgc gttcatctgc cgccgctgcg cgaacgtcgg
     5821 gaagatattc cccgtctggc gcgccatttt ttacaggttg ccgcgcgcga actgggcgta
     5881 gaagcgaagt tactgcatcc ggaaaccgaa gctgctctga cgcgtctggc gtggccaggc
     5941 aacgtgcgcc agctggaaaa cacctgccgc tggctaacgg tgatggccgc cgggcaggaa
     6001 gtgttgattc aggatttgcc cggcgaactg tttgaatcaa cggttgcgga gagtacttcg
     6061 caaatgcaac cggacagctg ggcaacgctt cttgcgcagt gggcagacag agcgctgcgt
     6121 tccggtcatc aaaatctgct ttccgaagcg cagccagagc tggagcggac gttactgacg
     6181 accgcgttgc gacatacgca ggggcataaa caggaagcgg cgcggctact cggctggggc
     6241 cgcaacaccc tgacgcgtaa gttaaaagag ctggggatgg agtgattcac agcttgtgtg
     6301 taaagattga ttattgagcg caaattgctg gtattttacg ctttactgtt ccgataagtt
     6361 cagtatgatc ttgcccggaa aacggggaga gtcattatgc tggaatcaat aattaatctg
     6421 gtatcgagtg gcgcagttga cagccacaca ccgcaaactg ccgttgctgc cgtgctgtgt
     6481 gccgcaatga ttgggctgtt tagttgagtt tcagattgct gatgaagtgc tctttgttca
     6541 tgccggatgc ggcgtgaacg ccttatccgg cctacaaaag caatattttc aacaggtttt
     6601 gtaggcctga taagcgtagc gcatcaggca atcttgttct cagcacattg atggcgcttt
     6661 cgttttctac tttgtaaacg aagcgccatt cactatttaa atcacccgtg agaactgctg
     6721 catccgcgct ttctggcgca gataggtatc aaagcacatg caaatgttgc ggatcagcaa
     6781 gcgacctttc gccgtcacct gtattccctt ctcatccaca tccaccagcc catcttttgc
     6841 taacggggcg agcagcttga gatcttccgc aaagtaatca gcgaagtgca aatcccactg
     6901 tttctcaata ggggcgtaat ccagacggaa gttgcagatg agcgacttaa tcacatcgcg
     6961 gcgaatacag tcatcacgcg ttagcgcaat accacgccac agcgcattgc cttgttcatc
     7021 cacttgctga tagtactgct tcaactcttt ctggttctgc gcgtagcagt cgccaatcat
     7081 gctgatggcg gaaacgccca tccccagcag atcggtatcg ccctgagtgg tgtagccctg
     7141 gaagttacga tgcagcacgc cttcacgctg ggccaccgcc agctcgtcat ccggacgggc
     7201 aaagtgatcc ataccgataa actgatagcc cgattgcgtc aggaaggcga tggtttcctg
     7261 caggatatcg agtttttgct gcggactcgg caggtcagca tctttgattt tgcgctgagc
     7321 agcaaaaatg gtcggcagat gcgcgtagtt aaagacactc agacgatcgg ggttcagctc
     7381 cgccacacgt ttcagggtaa aggcgaaact ctccggcgtc tgtttcggca ggccgtaaat
     7441 caggtcgatg ttggtggagg taaaaccaat ctcacgcgca tggttaagca gtgcaaagat
     7501 gaactcttca tcctgctcgc ggttaaccag acgttgcact tctttgttga agtcctgcac
     7561 gcccatgctc aggcgattaa agccttcggc gcgtaaatga tcgagtacat ccagttcgat
     7621 ttcccgcgga tcgacttcga tcgaaatctc cgcatcggca ttgaactgga agttttcgcg
     7681 cagcagcttc atcaggcggc tgatttgcgc tttattcaga tacgtcggcg ttccgccgcc
     7741 ccagtgcaat tggctgacgt gacgcccggc gaacagcggt gcacgatgga cgatttcttg
     7801 ctccagcgcg tccagatact gatcggcctt gtgctgctgg cgagtaacaa tcttattgca
     7861 accgcagaag taacaaagct tatggcagaa cgggatatgt acgtagagag ataatggacg
     7921 ctcaggatag cgcgccacgg cttgtaaaaa cgcctgttcg ccgaagtctt ctgaaaactc
     7981 cagcgcggtc gggtacgagg tgtatcgtgg cccggaatag ttatatttct ggatcagggc
     8041 cagatcccag tcgatttgct gtacagacat gctcactcct tccgatggcg tctctggcgg
     8101 ctacggcgta ccggcggatg cccgtttcgg gtcatcagcc ggttgcgcag ccacttttgt
     8161 cgccgcgaca accgctgtag tttaacgaat aaccacacca gataacatat aaccggaagg
     8221 gttatcagca gaacgggaag gcccacggtg gcaatccgtt agttaccccg cagcagacgc
     8281 atcatgtctt cttgcttctc gtcttcttcc tcttcttcgt catcgtcata agagaggccg
     8341 agtttctgca tcaactcatc aatacgatcc agtttagcat ccacccagga ttgctcttcg
     8401 gcgctcaggg tttcgcctgc ttccagacgt tccagcagcg catccagacg ctcatccgtt
     8461 tccagtaatt ccaactccgc ctgcggtgaa agcataggtt tctcactctt cggtttgtgc
     8521 tgtttggtga ctttttcagc cacgcccaat ggaatagggg ttttactgcc aatacgtgga
     8581 tcttttggtg cgttctggcc tttgctgcct gacgtggtgt ttccgcccgc tgcgcggctg
     8641 cccggcgcat gaccacgacg ttttttctga cgcttgcgat cacgagcttc ctgatccagc
     8701 tcctcgcgtg tttttcgacg cgcttttgca tgacctttgc tgcgtgagtt tgaagatgat
     8761 ggtttcataa tggttcgtct tttagccgtt ttattcagta tagatttgcg gcggaatcta
     8821 acagaaagca agcaaagaaa aaaggcgaca gattactctg tcgccttttt tcctgactca
     8881 taacccttaa cgggtcttac aatccttgca ggccgccaac acaccctgtt tttcctttag
     8941 ccggaacaat ccgtgttgat tccatttccg tttaattacg tctccggacg tttgtcttcc
     9001 tgacaatcct ctgtcttcgc ctcctggcgc tcctgaccct catcctgagt cattgttcct
     9061 gttagcgtcc tcgctctatg ggtgctactt tacgcctttg ctttaaacaa acaatcaacc
     9121 gccatcagta tttgcacatt ttcagagttt tatataaaag caattcactg atttatatag
     9181 cttatttgac atgcatcgca gaattttctc tgctatttcg cataaagact atcggcaatg
     9241 tctcacaaag cgtatggctt ttacttacaa cgctcacgcc gaaaaatccg ccttttatta
     9301 tggttcaggt ataatcgcca caaagctttg actgacggag acgaccgctt tgactaattt
     9361 gaattatcaa cagacgcatt ttgtgatgag tgcgcctgat attcgccacc taccttccga
     9421 taccggaatt gaagtggctt ttgcaggccg ttccaacgca ggtaaatcca gcgcgctgaa
     9481 cacgctgact aaccagaaaa gcctggctcg tacctcaaaa accccagggc gcacccagct
     9541 tatcaacctg tttgaagtgg ctgacggcaa gcgtctggtt gacttgcctg ggtacggtta
     9601 tgcggaagtc ccggaagaga tgaagcgcaa atggcagcgt gcgctcggcg aatacctcga
     9661 aaaacgtcag agcctgcaag gtctggtggt gctaatggat attcgccatc cgctgaaaga
     9721 tttggatcag cagatgattg agtgggcggt agacagcaat atcgccgttc tggtgctgct
     9781 gaccaaagcg gacaaactgg caagcggcgc acgtaaagcg caattgaata tggtgcgtga
     9841 agctgtactg gcgtttaacg gtgatgtgca ggttgaaacg ttttcttcgt tgaagaaaca
     9901 aggcgtggac aagctgcggc agaaactgga tacctggttt agcgagatgc agcctgtaga
     9961 agaaacgcag gacggcgaat aattttcttg ccttaatgct tgtgccggat gtggcgtatc
    10021 cggcccgtaa attcaataga ttacaattct accgttggcc tgataaacat agcgcatcag
    10081 gcattacgga tcttttcttt cgcccaataa aaaacgcccc agtcattact gactggggcg
    10141 gctaaaatat tcagccaaat ccgattacgt gaagtaaaag gtctgaaaga tagaacatct
    10201 tacctctgta ccctacgcga ctaactttac tcttttttgt tcagttcaga aagcactttt
    10261 tgtaattttt tttcagttgt tgcataggaa tttctaatag ccatcacaaa acgtgacagc
    10321 ttatgttgct tacttacgaa aaaagggcat gttcaggcga atcttagtgc gcctgatccc
    10381 agttttcgcc actccccact tccaccagca acggcacatc cagacgggta cagttttcca
    10441 tcagttgatg aatctgcttc gcgacggcat caacatcatc tttatgaact tcaaatacca
    10501 gttcatcgtg tacctgcatg atcatacgta cacgcggttg ctcagcctgt aaccacgcat
    10561 caacggcaat catcgcccgt ttgataatgt cggcggcggt tccctgcatt ggcgcgttaa
    10621 tggctgcacg ttcagccgct gcacgacgag caccattgct ggatttgata tccggcagat
    10681 acagacggcg tccgtccagc gtttcaacgt agccctgctc tttcgcctga gcacgggtgc
    10741 gttccatata ctccagcacg ccagggtagc gttcgaagta aaggtccatg tacttctgcg
    10801 cttctttacg tggaatgttc aattgccgcg ccagaccgaa agcactcatg ccataaatca
    10861 gaccaaagtt gatcgctttc gcgctacggc gttgctcgct ggtgacggtt tccagtggca
    10921 aaccaaacac ttctgccgcc gttgcccggt ggatatcttt tccttccgcg aatgcggtca
    10981 gcaagccttt gtcacgcgaa agatgcgcca taatgcgcag ttcaatctgc gagtagtccg
    11041 ctgagacaat cacataatcc tctggcgcaa taaacgcctg gcggatacga cgaccttctt
    11101 cgttacgcac cggaatgttt tgcaggttag gatcggttga cgataaacgt cccgttgcag
    11161 ttactgcctg gtgataagag gtatgcacac gcccggtttt cgggttgatc atcagcggca
    11221 gcttgtcggt gtaggtcgat ttcagcttcg ccagaccacg atactccaga atcacttttg
    11281 gcaacggata gtccagcgcc agttcttcca gtacctcttc cgacgttgac ggcgcgccac
    11341 ccggcgtttt cttcagcggt ttaatgccct gtttttcaaa cagaatggtt tgtaactgct
    11401 tggtggaaga aaggttaaat tcctcacctg caatttcatg cgctttcttt tccagctcag
    11461 ccagacgaag ggtgagctct tcagaatgat tgtgcagcac tttcggatcg atcttcacac
    11521 cgttacgttc aatgcgtgaa agcaccggca ccagcggcat ttcgatattc tcgaagacgt
    11581 tcaacggccc tttgtgtttt tgcagatctg gccacatttt cagatgcaac tgcaaggtga
    11641 catccgcatc ttcggcggcg taacgtccgg cctcttcgag ggcaatctgg ttaaaggtca
    11701 gttgattttt gcctttaccc gcaatctctt caaaagtgat agttttgtgc ttcaaccaac
    11761 gttccgcgag gctgtccata tcgtgacgcc cggcaacgct attgagaatg taggactcca
    11821 gcatggtatc aaacgcaatc ccacgcagct caatgccgta attcgccaga ataccgcgat
    11881 cgtatttcag gttttgcccg accttcagcg ccttttcatc ttccagcagc ggttttagca
    11941 actcgagtgc acgctcgcga gagatttgat cgggcgcatc aagataatca tgagcaaccg
    12001 gaatatatgc cgctacgcct ggctcgatag caaaagaaag cccgaccagg ttagcagaga
    12061 tgttatcaag gctgtcggtt tcggtatcaa atgcaaatac cggcgctttt tccagcttcg
    12121 caatccacgc tttcagtgtt tcttcatcaa ggatggtgac gtagttgtca taagaaatca
    12181 ccgttgccgt cacttctggc gcttcgtctg caacactggt ttcctgcggc ttcgcggctg
    12241 gttttgcccc tttggcctgt aaccatttgc ccgcttcgac atcagcagtc cagcgtttga
    12301 actcatactt tttgaacagc cccaacaact cttccgctgc cggttgctgc acttccagtt
    12361 gttcacaggt cagctccagt tcaacgtcgg ttttaatcgt cgccagctgg tatgagagat
    12421 aagcaacttc tttgttttgc tcgagcttcg ctgccattgt tttcgcgcca cggaagctca
    12481 acccagcaat tttttctggc tcggcataca gcgtatccag cccgccaaga ccttgcagca
    12541 atgcctgcgc ggttttttca ccgacgcccg gtacgccagg aatgttatcg gaggagtcac
    12601 ccatcagcgc caggaaatcg atgatcagtt ctggcggcac gccgtactta ttcaccacct
    12661 cttccggtcc gaggatggta ttcgtcatgg tgttgataag cgtaatattt ggcgtcacca
    12721 gctgcgccat atctttatcg ccagtgctga tcagcaccgg acgcccggct ttttcggctt
    12781 cgcgcgccag agtaccgata acgtcgtccg cttctacgcc agaaaccgcc agcagcggca
    12841 gtcccatcgc tttaaccatc gcgtgcaggg gttcgatttg tgctcgcaga tcgtccggca
    12901 ttggcgggcg atgtgatttg taatgttcaa acagttcatc acgaaaggtt tttcccttgg
    12961 cgtcaaagac caccgctgca tgcgtcggtt tatattgcat gatcagactg cgcagcatgt
    13021 tgaggacacc atacatcgca ccggtcggct cgcctgcgct gttagtcagc gggggaaacg
    13081 cgtgatatgc gcgataaaga taagatgaac catctacaag gataagtgga ttttggggga
    13141 tctgaaccat aatgtccgtg cctgtttatc agattatggg taaaggatgc cacagacagg
    13201 atgaaaacat gagtttttca ccgcttttcg tgaaaatctt ttgtagatct tctggatcgc
    13261 tcgcaaatcg tcatgtggat aactttgtgc ataaattatt tacatcgctt ttaatttatt
    13321 atcatgcttt ttacagcatg atgtttgtct ttgtttttca tgcagttaaa aataaagcca
    13381 tccctaataa ttgagataat gggatttttt ctaatgtgga tagatatgga ttatttttct
    13441 ccttaaggat catccgttat ttgggtcgtt ttctgctaaa atctgcgcct tgcgcttctt
    13501 tagcgcactg tctatggcta accttttgaa taaattcatt atgacgagaa tactcgctgc
    13561 gataaccctt ttgctgagta tcgtattgac cattttggtc actatttttt gttccgtccc
    13621 gatcatcatt gccgggattg taaaactatt gctacctgtg ccagtcattt ggcgaaaggt
    13681 ttcgcgtttt tgtgatttta tgatgtattg ctggtgtgaa ggtctggcgg tattactgca
    13741 tctgaaccca cacctgcaat gggaagttca cgggctggaa gggttaagta agaagaactg
    13801 gtatctgctt atttgtaacc accgtagttg ggcagatatt gtcgtactgt gcgtgctgtt
    13861 tcgtaagcac attccgatga ataaatattt cctcaagcaa cagctggcct gggtgccatt
    13921 tcttggcctg gcgtgctggg cgctggatat gccgtttatg aagcgttatt cccgcgctta
    13981 tttattacgt catccggagc gacgtggtaa agatgttgaa accactcggc gttcttgtga
    14041 aaagtttcgc ctgcatccca ccaccattgt caatttcgtt gaaggctccc gtttcacgca
    14101 agaaaaacat cagcaaaccc actccacttt tcaaaacttg ttgccaccaa aggcggcagg
    14161 cattgcgatg gcgctaaatg tactgggtaa acaattcgat aaactgctta atgttacgct
    14221 gtgttatccg gacaataacc gtcagccgtt cttcgatatg ttaagcggta aactgacgcg
    14281 gattgtcgta catgtggatt tacagcctat tgccgatgag ttacatggcg actacatcaa
    14341 tgacaaaagc tttaagcgcc attttcagca atggctgaac tcattgtggc aggaaaaaga
    14401 ccggcttctg acgtctctca tgtcatcaca gcgtcagaat aagtaagttt tactcgcctc
    14461 tgaatgagca gaggcgagtg agtattttaa tgaacaaaac gtccggcacg agacataaat
    14521 tcttcttcgc tcatctcctg tccgttaaac acaactttac ccggtgtata acgcagctgc
    14581 agcgaggcgg tattgttgtc aatcgtgatt aactgaaaca tctgacccaa tgtcatcatc
    14641 ccgctgattt gtttatcagc ctgcttttgc gctttttccg catccattcc ttcagataaa
    14701 ttaagctgtt ttgcggtttc tgtgacgaca ttaagcggta acttcacatc aaaattgagc
    14761 aatttgatat ctttgtttgt ggatgatgaa gatttggctg ggtcggcaat actgatatcc
    14821 agattggcat ttagttcgcc gagtgcgttc ttccatctta ccggttgttt aatggtcggc
    14881 tcactttttt gtaataacgg taaatactct ttgaacaatg cagcattcac ttcttgcaga
    14941 gcaacttcat cgtttgctaa ctcagggtgt acagcaagct gcttctgcat agcaatgtta
    15001 tactggatga taaattggcg cactgctgag ggatcaatag attcagcaat caatgaccac
    15061 tcaccactac cgagttgctg attccctttt gttaacttat caagttcgta ggtcagattg
    15121 acattgatat aatctttgtc tttaacgcga tccagcatgg tttttgcaac gaaagcatcg
    15181 atttgggcaa cgtcttcccc gtgattgata atgttaatct gattaaattt cgactcgctt
    15241 tcaccaaccg gaaatttctt ttcttctaat cgcgccagag aatcaatggt aaaacttttt
    15301 gccgttactt tgttcattgg tgataattgc agaacaatat cttcgacttt gccttcaata
    15361 tccaggttag acaatgaact accatcacca ttaaaagtaa actggccctc accaaaagaa
    15421 aactttccat actctgcagg ggcaacatct aatgtcgtag agaatgagcc accaaacgcg
    15481 aatcgcaatg tcgcttccac gggcgattta tttttcgcgg cgataaatag cggttgtgtc
    15541 agttcattat tcactaagtt cacttttgcc gctgccagtg ctgggatgac attaccatgc
    15601 attaacattg tgatgggcaa cggaccgtgc tcaacatcca catcaaatac aactttctgg
    15661 cctggcttga tattgagatc gggtgcaccg ttatcgaatg tcatttgcat ttgaaaacga
    15721 gaattaaaaa aacctttgtc aaaattttta taacttaacg tcatatcgta ggcatgttca
    15781 cctttctttt tagcatcgtt aaaatcttta ataaactttt cgacaccagg ctgaatttgc
    15841 gtacctgtgt accatgtgcc accaccccag attacggcta acgcaacaat aacacctgta
    15901 gctgacttac gtatcatata aatattccct gataaaacag caaaacttta aatatccgct
    15961 tatgctgaaa taatgtccag cggcaggacg cattatcaga atgtatttat atttattaaa
    16021 taataaaaaa aaccgtgaat attcacgg
//
LOCUS       DAZPDU010000094        15991 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZPDU010000094 DAZPDU010000000
VERSION     DAZPDU010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15991
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.095"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(74..226)
                     /locus_tag="ACHURS_002691"
     CDS             complement(74..226)
                     /locus_tag="ACHURS_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR1238381.1"
                     /translation="MLTKYALVAIIVLCFTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(491..1225)
                     /gene="cysH"
                     /locus_tag="ACHURS_002692"
     CDS             complement(491..1225)
                     /gene="cysH"
                     /locus_tag="ACHURS_002692"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFR1238382.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(1300..3012)
                     /gene="cysI"
                     /locus_tag="ACHURS_002693"
     CDS             complement(1300..3012)
                     /gene="cysI"
                     /locus_tag="ACHURS_002693"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFR1238383.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIITGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(3012..4811)
                     /gene="cysJ"
                     /locus_tag="ACHURS_002694"
     CDS             complement(3012..4811)
                     /gene="cysJ"
                     /locus_tag="ACHURS_002694"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFR1238384.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PQTPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5130..5492
                     /gene="queD"
                     /locus_tag="ACHURS_002695"
     CDS             5130..5492
                     /gene="queD"
                     /locus_tag="ACHURS_002695"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFR1238385.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5570..6841
                     /locus_tag="ACHURS_002696"
     CDS             5570..6841
                     /locus_tag="ACHURS_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR1238386.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGVTTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLEP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            6832..7092
                     /locus_tag="ACHURS_002697"
     CDS             6832..7092
                     /locus_tag="ACHURS_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFR1238387.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7109..7684
                     /locus_tag="ACHURS_002698"
     CDS             7109..7684
                     /locus_tag="ACHURS_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFR1238388.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7832..8692)
                     /locus_tag="ACHURS_002699"
     CDS             complement(7832..8692)
                     /locus_tag="ACHURS_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFR1238389.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(8689..9468)
                     /locus_tag="ACHURS_002700"
     CDS             complement(8689..9468)
                     /locus_tag="ACHURS_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFR1238390.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9446..10396)
                     /locus_tag="ACHURS_002701"
                     /pseudo
     CDS             complement(9446..10396)
                     /locus_tag="ACHURS_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417251.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(10383..11816)
                     /locus_tag="ACHURS_002702"
     CDS             complement(10383..11816)
                     /locus_tag="ACHURS_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000059289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFR1238391.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTDFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGINLAG"
     gene            complement(11886..12702)
                     /locus_tag="ACHURS_002703"
                     /pseudo
     CDS             complement(11886..12702)
                     /locus_tag="ACHURS_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            13020..14297
                     /locus_tag="ACHURS_002704"
     CDS             13020..14297
                     /locus_tag="ACHURS_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFR1238392.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALGMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            14324..15810
                     /locus_tag="ACHURS_002705"
                     /pseudo
     CDS             14324..15810
                     /locus_tag="ACHURS_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
BASE COUNT         3758 a         4220 c         4035 g         3978 t
ORIGIN      
        1 tataaattaa aaaagggtgc aggagaccaa accatcacaa ttcagtacgc ggggttttac
       61 ccccgcgtgg tggctacttc ttcgattcgt aagcgagaac tgccttaaac tccataccac
      121 gttctctgat actcaactca cacaacgagt cgcctaccat cagcgtgaat cccagtactg
      181 taaaacacag cactatgatt gccacaaggg catattttgt cagcatgtct tgcctccctg
      241 caaagaagag actaacattc gaattgtttg ggttcgaacg ctggcctcag gttgatagaa
      301 atatcgcctg gggcttttgt ccatctggaa cctcgcgaat gcttaacgcc agacagcctc
      361 aagcacccga cgccattcta tacctgataa ttcttccagc ggcgttcacg tttaaaatca
      421 ggaatactgc tcgcaaaata aaaacgcccc catcatttct gacagaggcg tttaatttat
      481 ccggcaatat ttacccttcg tgcaatccac attccctttt taagccaaag aaacgtgttt
      541 cttcttccga catgccaggt tcccatttac gggttgtatg ggtatcaccg accgataaat
      601 atccttcatc ccataatggg tgatatttca ggccatgttt ttgcaggtac tgataaatag
      661 ttcggttatc ccagtcgata atcggcagca ctttaaatac gccacgctga attgccagca
      721 ccggtaaatt ggcgcgactg ccggattgtt cacggcgcag gccagcaaac caggtttgcg
      781 cgttaagttc tttcagagcc cggttcatcg gttcgacttt gttgatgtca ttgtactttt
      841 caatgccttc aacgccctgc tcccacagtt ttccgtagcg tgcttcctgc caggctgcgc
      901 tttcggtagc acggtacact ttcaggttga gcttgagttt gtccgttaac tcgtcaataa
      961 agcggtaggt ttccgggaac aagtaacccg tatcggtgag gatcaccgga atatccgggc
     1021 gaatttgatt caccagatgc aggctcaccg ccgcctgaat gccaaagctg gaagaaagta
     1081 catattcacc gggcagatta tccagcgccc aggctacgcg gccttcagcg tccagttttt
     1141 ccagttcggc gttagtttcc gccagcgcca gaatacgatc taccttcggc agttcgttca
     1201 gggcgtttag atcgagtttg gacatagatt cctcactgtt tgccttgcct gatgcgacgc
     1261 tcacgcgttc ttatcaggcc tacaagaccg ggctgatggt taatcccaca aatcgcgcgc
     1321 cggatcgagc accgggcgaa tgatgcccgc acgcaccgta aagtcgccga agccttcacc
     1381 cgcttcgcgc tctttcgccc agcgccctat cagttcatca agcgacgcca ggatttccgg
     1441 ctcggtgatg ttttctttat acatccgtgg gatacgtgtc ccaatgcggt tgccgccaag
     1501 atgcaggttg tagcgacccg gcgctttacc caccaggccc acttccgcca gcatcgcgcg
     1561 accacaaccg ttcgggcagc ctgttacacg catcacgata tgctcatcgc tgacaccatg
     1621 tttcgccatt aaattatcga tgttgtcgat aaaagacggc aggaaacgct ctgcttccgc
     1681 catcgccagc gggcaagtcg ggaatgacac gcaggccatc gagttttcac gctgcggcgt
     1741 gacggcattc attaacccgc tctctttggc gatcttctcg atcttcgctt tctcgctttc
     1801 cggtacaccg gtgatgatca gattctggtt cgccgtaatg cggaaatcgc ctttgtggat
     1861 cttcgcgatc tccagcaggc cggttttcag cggacgcccc ggataatcaa ggatgcgacc
     1921 attttcgata aacagcgtca ggtgccagtt atcatcaatg cccttaaccc agccaatacg
     1981 atcgcctcgt ccggtgaact catatggacg gatcggttca aatttgatcc ccgcgcgacg
     2041 ctccacttcc gctttaaacg tctcaacccc cacgcgctcc agcgtgtatt tggttttggc
     2101 atttttacga tcggttcggt taccccagtc acgctgagtt gtcacgacgg cttcggccac
     2161 cgccagcgta tgctccagcg gcagatagcc aaactcgctc gccgtgcggg cgtaggtttt
     2221 cttgttgccg tgttcgatag aaagcccacc gcccaccagc aggttgaagc ctaccagctt
     2281 gccgttttcg gcgattgcca cgaagttcat gtcgttggca tgcagatcga tatcgttctg
     2341 cggcgggatc actaccgtgg ttttgaactt acgcggcagg taggtctgac caaggatcgg
     2401 ttcttcatca gtagtggcga ctttttcctg gtcgagccag atctccgcat acgcgcgggt
     2461 acgaggcaac agatgctcag aaatcttctt cgcccactcg tacgcttccg cgtgcagctg
     2521 cgactcgtaa gggttcgagg tgcagagtac gttacggttc atgtcgttag ctgtcgccag
     2581 cgcatcaaga ccgaccgagt gcagcatctg gtgcaccggt ttgacgttct ttttcagaat
     2641 gccgtggaac tgaaacgtct ggcggttggt caggcgaatg ctgccataga tggtgttttc
     2701 accggcaaat ttgtcgatcg cctgccactg tttagtggta atcaccccac ccggcagacg
     2761 acagcgaagc agcatcgcgt ggcgcggctc cagcttctgt tcagcacgtt cggcacggat
     2821 gtcgcggtca tcctgctgat acatgccgtg gaagcggatc agcaggaagt tgtcgccctt
     2881 aaagccgccg gtcagaccgt cgtttaaatc ttccgcaatg gtgccgcgca ggtagttgct
     2941 ttcaagcttc atgcgctcgg cgtctgtcag ttttccttcg accactaaag gccctggatg
     3001 tttttcgctc attagtagac atctcgctga taacggcgct ctacgcgcag ctcacttaaa
     3061 aattcatccg ccgcttcggt gtccatgcca ccaaattcgg caatcacttc cagaagtgcc
     3121 tgctcaacgt ctttcgccat gcgattagcg tcgccgcaga cataaatgtg ggcaccgtca
     3181 ttgatccagc gccacagctc cgcgccctgt tcgcgcagtt tgtcttgtac ataaactttt
     3241 tctttttggt cgcgcgacca ggcaagatcg atacgcgtca gcacgccctc tttgacgtag
     3301 cgctgccact ccacctggta gaggaaatct tccgtaaagt gcgggttgcc aaagaacagc
     3361 cagtttttac ccggcgcttc gtcggcggcg cgttgctgca taaaggcacg gaacggcgcg
     3421 atgccggtac cggggccaat cataatcact ggcgtttgcg ggttagcggg caggcggaaa
     3481 ttgtcgttat gttcgataaa tacgcggact tcgccctctt cttccacgcg atccgcgagg
     3541 aagctggagg caccaccggc acgggcgcgg ccttccacgt cgtaacgcac cacaccaacg
     3601 gtgacgtgta cttcgttctc gacttccgcc tgcgaggagg cgatggaata caggcgcggg
     3661 gtcagcgggc gcagcaggtt aatcagcgct tcggcatcca gttgcgccgg agagaaacgc
     3721 accatgtcga caatcggcgt ggtcgcggcg taatgctgta acttcgcttt atcgcccacc
     3781 agcggcagca gcgtttcgct gcgggtaagc gtggcatagt tctcaacaat gttggcggtg
     3841 ttgacggtca gttcgaagtg ccactgtagc gcttcgttca gaggcaacgt tttgccctcg
     3901 acggtgacag gttcatcgcc tttcagccac agcagttcga caagttcttt caccagtgcc
     3961 gggtcgttct gataccagac acccagcgcg tcacccggct ggtaacgcag gcctgaatcg
     4021 cctaagtcaa tttcgatatg acgaacgtct ttttcagagt tacgcccggt aattttctgg
     4081 ttaaccgaaa ggctcgccac cagcggcgcg tctttgctgt acgggctggt gtggatttca
     4141 tttaccgcgc cagtagcgac ggattgcgaa ggtgccgcga caggcgcacg cgatttaagc
     4201 gcatcaacca cgcgggcgcg ccactcgctg gcagcagcct ggtattcaac atcggcatcg
     4261 acacggtcga gcaggcgttc accacccagt tccgccagct tgctgtcgaa atctttcccg
     4321 gactggcaga aaaattcata agagctatcg ccgaggctaa acacggcaaa cgcggtgttt
     4381 tccagctttg gcgctttttt ggagaacagg aacttatgca gcgcgacggc ttcttccggc
     4441 ggttcccctt ccccttgcgt tgacgtcact acgatgagca gtttttcgct ggcgatttgt
     4501 ttgaatttat agtcgcccgc gttcaccagc ttaacgttca gttttgctgc taataaatca
     4561 tcgcgtaatg cttcagcaac ccggcgcgca ttgccggttt gcgaggcgga gataatagtt
     4621 atacccggca tttctgcggc tggcgctggc gtcgctgcaa gcgcagcagg ctgctgattg
     4681 agtacgcccc agaaatagcc agaaacccag gcaagctggg taggcgttaa atcggtcgtg
     4741 gccgcctgaa ggcgtgccag ttgctccggg ttcaacggaa gcaacgcgga aggtgggacc
     4801 tgtgtcgtca tgcgtcgtta tgttccagta agcaaagctg tttctgcgcc ctgtcagcgc
     4861 ccataaaaca gaagagaaga taaggttaac ggggtgaacg gtgtggatta aagacgggat
     4921 agcgataact aataaccaaa tcgactaacc tgttttagca atagtcttta acaacaaaat
     4981 agattaacca acccaatgaa aaacaaatga atttagccaa tcattaagat aaatcagcga
     5041 ttttgcgcaa caaagtcgtt ttagataatg cgaaaaaaca gcctttccgg tactctacgg
     5101 cggttttatc gtcctgtaga gaaattatga tgtccaccac gttatttaaa gatttcacct
     5161 tcgaagccgc tcaccgctta ccacacgtcc cggaagggca taaatgtggt cgcctgcacg
     5221 ggcattcctt tatggtgcga ctggaaatta ccggggaagt cgatccgcat acgggctgga
     5281 ttatcgattt cgctgaactg aaagcggcgt ttaagccaac ttatgagcgc ctcgatcacc
     5341 attatctcaa tgatattcca ggtctggaaa acccaaccag cgaagtttta gcaaaatgga
     5401 tttgggatca ggttaaaccc gttgtgccgc tgttaagtgc ggtgatggta aaagaaacct
     5461 gcaccgcagg gtgtatctat cgcggcgaat gataagagtg tgtcggcggt caatttccct
     5521 taagtaacgc tatgttaggg tgttgtgttc tggatatctg gggcatgaca tggaagacga
     5581 ctgcgacatt attattattg gtgccggtat tgcaggcacc gcttgcgcgt tacgctgcgc
     5641 gcgagcgggt ttatccgttt tgttactgga acgcgctgaa atccccggca gcaaaaatct
     5701 ttccggcggg cggttatata cccatgcact cgcggaactc ctcccgcaat ttcatctgac
     5761 cgcgcctctt gaacgacgca tcactcacga aagcctttcc ctgttaacgc ccgatggcgt
     5821 aacgacgttt tccagcttac agcccggcgg tgaatcctgg agtgtattac gtgcacggtt
     5881 cgatccgtgg ctggttgccg aagccgaaaa agaaggtgtc gaatgcatcc ccggagcgac
     5941 ggtggatgca ctgtatgaag aaaacggcag agtctgtggc gttatttgtg gtgacgatat
     6001 tctccgcgcc cgttatgtgg tgctggcaga aggtgccaac agcgtcctgg ctgaacgtca
     6061 cgggttagtg actcgtcctg ctggcgaagc gatggcgttg gggatcaaag aagtgctgtc
     6121 gctggaaaca tccgctattg aagaacgttt tcatctggag aataacgaag gcgcagcgtt
     6181 gctgttcagc ggcggaatct gtgatgactt acccggcggc gcatttcttt atactaatca
     6241 acaaacgctc tcgttaggga ttgtttgccc gctctcttcc cttacgcaaa gtcgtgttcc
     6301 ggcaagcgag ctgctgactc gctttaaagc gcatccggca gtgcgcccgc ttatcaaaaa
     6361 cacggaatca ctggagtatg gtgcgcatct ggagccagaa ggtggcttgc acagtatgcc
     6421 ggtgcaatac gccggtaacg gctggctgct ggtgggcgat gcgttgcgca gttgcgtcaa
     6481 taccggaatt tccgtgcgcg gcatggatat ggcgctgact ggcgcacagg cggcggcaca
     6541 aacgctaata agcgcctgcc agcaccgcga gccgcaaaat ctgtttgcgc tttatcatca
     6601 caacgtagag cgcagcctgc tatgggatgt tctacaacgt tatcagcatg ttccggcgct
     6661 tttgcaacgc cctggctggt atcgggcgtg gcctgcgtta atgcaggata tttcccgcga
     6721 tttatgggat cagggtgata aacctgttcc accgctgcgc cagttattct ggcgtcattt
     6781 acgtcgtcat ggcctgtggc atctggcggg cgatgttatc aggagtctgc gatgtctgta
     6841 gcccataatc tctggcgcgt tgctgatgcg ccgcacattg ttccggctga ctccgttgag
     6901 cgtcagatgg tacaacggtt gattaacgcc tgtccggcag gtcttttttc gctcacaccg
     6961 gaaggtgact tacgcgttga ttatcacggc tgcctggagt gtggcacctg ccgtttgctg
     7021 tgcgacgaat ccacactaca acagtggcgc tatccgcctt ccggattcgg catcacctac
     7081 cgctttggat aaataataag gataatttat gcccctctta cacctactcc gccagaatcc
     7141 ggtgattgct gccgttaaag ataatgccag cctgcaactg gctatcgatt ctgaatgcca
     7201 gtttatttcc gtgttgtacg gcaatatctg caccatcagt aatatcgtta aaaagattaa
     7261 gaacgccggg aaatatgctt ttattcatgt tgatttgctg gaaggcgcgt caaataaaga
     7321 agtcgttatt cagtttttga aactggtgac cgaggcggac ggcattatca gtaccaaagc
     7381 ctcaatgttg aaagcggcaa gagcggaagg ttttttctgt attcatcgcc tgtttattgt
     7441 tgattcaatt tcgtttcaca acattgataa gcaagttgcg caatcgaatc cggattgtat
     7501 tgagatcctg ccaggctgta tgcccaaagt gctgggctgg gtgacagaga aaatccgcca
     7561 accgctgatt gccggtgggc tggtgtgcga tgaagaagat gcgcgtaatg cgattaacgc
     7621 gggtgtcgtg gcgctttcca ccacgaatac cggggtctgg acgttagcga aaaaattact
     7681 ttgacgggat aatcgtaacc aattgaattt ggtttgattt gtaggccgca cgccacatcc
     7741 gacattcagc gcctgatgcg acgcttgacg cgtcttatca ggcctacaag tccccaggcc
     7801 agaaccgtag gcaggatcat ccggcaacac gttattggca atctgcgtga atgtttgtca
     7861 ccagtgcctc cagcaccact ttccagtcat caaccacgcc aacatctgcc tgtgaaaata
     7921 ccgcagcgct ggcgtcatga ttaatcgcca ccacaaactt gctattgcgt acgcccgcca
     7981 ttaacgctgc tgcgccagaa gcgccaacaa caatacacac ctcaggtgcc agcagatgtc
     8041 cggaaatacc aatcactttt tcagcatcaa cgccaccgtt catcactcgc gcccgactgt
     8101 agcccacttc ggctcccagc ttttctgcca gcatagcgat ttcctggtta tctgcctctc
     8161 cgccctgccc caccaccagc acccgtctgg cctcagctaa tgggtcgcga gtaacatttt
     8221 tcagatcctc tgtgctaacc agccagtccg gaagcgcacc cgggacaatg ttcagttgtt
     8281 gcattccaga gggcaacgtg gcgtttttcg ctgctcccgc ctgacgcgcc agggaaaggc
     8341 atagcgggcg tttttctgtt tgcagcgtcg ccgttagcgc atttccccag tgggatttgc
     8401 gcacgcttac ggttggaata tctaacgaag tcacctggca gatgcttgca ccatgtaaac
     8461 gccaggccag ccgggtcgac agttcatcgc caaacgttcc cggcggaaag agaacaacgt
     8521 ccgcaggcgt tcgctgccac tgttccacca acgcatccag cacctgttcc gccactacag
     8581 gctgaggttc aatctgccag tgcgccaggg tacaaccgct gaaatcctgc gccgccagcc
     8641 agcttgctat tgcggcattc tcctgattta ttgtcacaat tgcgatattc atggctgcat
     8701 cctttggcgt aaatagtcct gccatagctt ttgagctttt tctgctaccg tttgcccatc
     8761 aatcagcgtc gctccgcgtc gttgctctgc tcttgccagt tgcagacact gcatcgctgg
     8821 catttcggca gcaacagttt tacggataat ttctgctttc cccgcagcca ttcgctggcg
     8881 cattcccggt actggcaagg ccacttcacc gcactgacgc acggcaatca ccgcaggcaa
     8941 gcgaacccgg caacaacgca acccatgttc agtacgctgt tcgagggtga taaacagcgc
     9001 gtcgagagtg aaacgctcca cctgggtgaa gcagggccag cccagcattt ccgccagcaa
     9061 aaatggcgtt tgcccattct gcccttcgct gctttggcag ccggtaatta tcagatccag
     9121 cggattctga tgctgccatt cggcaatatg gcgagcgaca aattccggcg caaagcgcag
     9181 atctgccgcc gtctccagca atacagcttc ctcaaaccca agagccatga gatagcgcag
     9241 ccagtgcaat gcccgttcat cccccatgct caacgcggtt aaagacatcg gcgtaccgtt
     9301 ttttctctgc gccaacagca gcgcggcagc ggcctgttca tcagcaccga gtaaacttcg
     9361 cagtagcgaa atatccggtc cgcttttacc ctgagccgcc gcctgccact ctttttccgc
     9421 cagcattccg gcatccggtt cggctttaaa cgctaacaga atgttcattc gcacctccta
     9481 catttcccgc cgccaccagc gggagggctt tagtttctgg tgcccatagc caggtcacaa
     9541 caaaatcaac caacaacact gtcgccagga gcaataaggt gacttgcatt ccccactgcg
     9601 ccagcaccca cggcagcagg ccagtactta ccgccgcgcc aagtcgactc atggcggtgg
     9661 caaaaccgac gcccagcgag cgaatgtcag taggaaaact ttccgcaggc aaaatgccca
     9721 ccagattact gactgccgaa atggtggtgc tgaagagaac aaaaagcagc agcgttaatg
     9781 aactgccgga aggcaaacag gccatcacta ccagcgttgc cgccagcagc aaaaaacttc
     9841 ccagcaaaaa tttgcgatgt gccagcaggt gcgtcagaac taatcccagc agcgcgccca
     9901 caattaacaa cgcattaagc atcaggctgg cagtcagcgc atcttccaga ccaatagtct
     9961 gggcgatagt tggcagccag gtataaatca caaaccatgg gattacgagg cagacaaaga
    10021 agacgctgtt aaacgccgtg cgccgccagt aacgcgaaga gaacaaggtt ttgatgtgtt
    10081 tatgggtcgc cgttaccact tcatcgccca gtaaaacatg gggaccaaaa tagcgatgca
    10141 cgatagcgtg agcttctgca aaacgcccct ggcgcagtag ccagcgtggt gattctggcg
    10201 ttccccagcg taataacgta atcaacaacg cgggcagagc tgccgatgcc agtagccagc
    10261 gccaggcctc cgggttttcg gaaataaagt gatgtccggc aatacttgcc agcacatagc
    10321 ctacggtcca caccacgctg aatgcgccca gcaaaatacc gcgatggcgg cgcggggaaa
    10381 attcagccag caaggttaat gccattagga tcgaactgct ttttcagccc ttccagcaac
    10441 gcccacgcgc tgccgtgttc cagtttgctc cagtgaacgc gatgtttacc gataccgtgg
    10501 tggtgcacca tcgaaccgcc gaggcgaatg gtttcttcac agatgatctt gttgagcgga
    10561 ttgtggtact tgtcgatttc ctcttccggc ttacagtcaa cgacgttgta atcgtagacg
    10621 aagtacatgt tggtgccgtt ctgatagcta tgagaggaat gaccgcccag catggtgatg
    10681 tcgtcggcgt gcgggaactc agtacgaata cggttaataa cgctttcgta gatttcgtgg
    10741 atgcagctcc agcagccgga cacttcagtg gtaaagccca tgttgccggt tttgaggatc
    10801 tgcacacgtt cggcagccac tttatccggt ccccagttca ggttgttaaa ccaggtttcg
    10861 atcagcttgc tgtccacgcg ctggcattgc gggtagcggg caacgatttc cgcaatccct
    10921 tcgcccgtcg ccttcgcaat gcgagggtta ccttcagcca taaagatcag cacgcatttt
    10981 ccgtctgcaa aatgggtgaa gtgttgggtg ccatcttcag cgtcatacaa acgagcgatc
    11041 gacggacgat acccttccac catgacttca cgcaggatgt tgaagtcggt tttcatgtct
    11101 tccaggatat agccgtagaa gaggttgttt tccggggtga atttaaagat tttcactgtt
    11161 acttcagtga tatagcaaag tgcaccttcg ttaccgatga tgatgtggcg aatgtccggg
    11221 ccagccgcgc ggcgtggcac gtttttaatg cgtgtgacgg tgccatcagc caatactgct
    11281 tccagaccaa cgaccatatc ttcgattgcg ccgtagagtg tggagaactg cccgatactg
    11341 cgggttgcta ccaagccgcc catctgcgcc agcggctttg actgcggaga atgccccgtg
    11401 gtgtaacctt tttcacgcaa cgcattttcc agcacctcca gcggaacgcc acattgcgcc
    11461 gtcgcctgca tattctcaat atcaatatta atgatttgat tcatggcgga gccgtcaagc
    11521 accaccgagt tttccacaac ggtttccagc ccaccttcag tggcggaagc gccggtacgc
    11581 ggcacaccgt taattttgtg cgcattcata aaattcagca cacgggatac ttgctctgtg
    11641 gaaccgagtt ttacgaccgc tgccggaatc ggcaaagtat aaatgccatg aatatccgga
    11701 aatttacgaa aacggtcaat actgttcttc tttaatactg tttcatcggt aattacgcga
    11761 tctgcaccaa caatttcctt tagttggtcg acaatcgctg cgcgagataa agacatagta
    11821 attccttcct gataataaaa atggaataat cattaataag agaaaacaga aaccaccgtt
    11881 atggattagc gcactaaata accaccattg accactaata aatgcccatt gacataattt
    11941 gaagccggac ttgcgaggaa tacagctgcg cccattaaat cctgagtatc gccccaacgg
    12001 tttgccggaa tatgatcaag aacgcgctga ttggtttctg gattactgcg tgtcgccagc
    12061 gtaatatctg ttgcataata gccaggagca ataccattta cctgaatatt atattgacct
    12121 agttcatcac aataagcttt ggtgaatccg gcaagagcat gtttagtggc agaatatgca
    12181 ggtgaccatt gtccacctaa gtaagagaac aatgaacaga tattaataat tttgccgctt
    12241 ttctgcggga tcataatttt tgcagcttca tagcttaact cgaatgcggc ggtcaggttc
    12301 acatcaatca tcggatccca gtcggcacga ccgaagtcca gcaccttatt cagcttacaa
    12361 ataccggcat tgtttaccag aatatcaact gtaccgaaac gctcacagca agcagcgata
    12421 atcttctgcg gcgcgccttc tgcggtgata tccacctgca tgaagtccac ctcaacaccc
    12481 tgtttttcaa acatttcctt tgtttcgccg ttatctttca atcatttcct ttgtttcgcc
    12541 gttatctttg acgaaactag gaataaagat atttgcgcca gctttggcca acgccatggc
    12601 aaatgcctgg cctaaaccgc tattcccacc ggtaacaatt gcggttttac ctttcagtga
    12661 gaaaaaatcc attgagaacg cattgagaga ttcgattgac atagttagct ccaatttccg
    12721 taaggcaaaa aaaagaaagg taacttcccc cgcgagaggg aagttacctt tctcattatc
    12781 tctggtaact ggagataatt tttagcataa cggcataact gctaatgtga tcgtaatcac
    12841 agtgtgatat tcgtattgtc aaatagtttt aaaaagcaga agcgtgatta ctctcacaca
    12901 acacctctgc tttccatgtt atttcttatt gcaattacta gagacctgag aaaagtaatc
    12961 cacctgaaag ggcggcttac ttttctcttt tttttgtcta ttacaaatgg gaatacgata
    13021 tgcaacacaa ctcatatcgc cgttggataa ccctcgcgat aattagtttt agcggcggcg
    13081 ttagttttga cctggcttat ttacgttata tttatcaaat tcccatggcg aaatttatgg
    13141 gattcagcaa taccgagata ggtttaataa tgagtacctt tggtattgcg gctattattc
    13201 tttatgcccc cagcggcgtt attgccgata aattttcaca ccgcaaaatg attacttccg
    13261 cgatgatcat taccggatta ctgggtctgt taatggcaac gtatccaccg ctgtgggtaa
    13321 tgctctgtat tcaggtcgcc tttgcgataa cgacgatttt aatgctgtgg tcggtgtcga
    13381 ttaaagccgc ctcgttgctg ggcgatcata gtgagcaagg gaaaattatg ggctggatgg
    13441 aagggctgcg cggcgtcggt gtaatgtcgc tggcggtgtt taccatgtgg gtcttttctc
    13501 gctttgcacc ggatgacagc gccagcctga aaacagtcat tatcatctac agtgtggttt
    13561 acatcttgtt ggggattctg tgctggtttt ttgttagcga taacaacaac ctgcgcagtg
    13621 ccaataacga agaaaaacag tcattccagc ttagcgacat ccttgccgtt ttgcgtatca
    13681 gcaccacctg gtattgcagc atggtgattt ttggtgtctt cacaatctac gccattctga
    13741 gttactccac caactatctg accgaaatgt acggcatgtc gctggtggca gcgagctaca
    13801 tggggattgt gatcaacaaa atcttccgcg cgctgtgcgg cccacttgga ggcattatca
    13861 ccacctacag taaagtgaaa tcccctaccc gcgtgattca aatcctttcc atcatcggtt
    13921 tgctggcgtt gacagccctg ctcgtcacga actctaaccc ccaatcggtc gcgatgggga
    13981 ttggcctgat tttactgctg ggattcacct gttacgcctc acgcgggctg tactgggcct
    14041 gccccggcga agcgagaaca ccgtcttaca ttatgggcac cacggtaggt atttgttcgg
    14101 tgattggatt cctgccggat gtcttcgttt acccgattat cggccactgg caagacaccc
    14161 tgcccgctgc agaagcctac cgcaatatgt ggctgatggg catggcggcg ctcggcatgg
    14221 tgattgtctt tacctttttg ctgttccaaa aaattcgtac tgctgatagc gcccccgcaa
    14281 tggctagcag caagtaagcc aggaaaatta caggagaaaa atcatgtcga aaaaatacat
    14341 catagggatt gatggcggaa gtcagagcac aaaagtggtg atgtacgatc tggaaggtaa
    14401 tgtggtttgc gaaggtaaag gcttattaca gccgatgcac acgcctgatg ccgatactgc
    14461 agaacatcct gacgacgatt tatgggcatc attatgtttt gcaggtcacg atttgatgag
    14521 ccagtttgcc gggaataaag aagatattgt tggcatcggc ctgggctcca tccgttgctg
    14581 ccgtgcgtta ttgaaagccg acggcacgcc agctgcgccg ttgattagct ggcaggatgc
    14641 acgcgttaca cgcccttacg aacatacgaa tcctgacgtg gcgtatgtaa cctctttttc
    14701 gggttatctg acgcatcgct taaccggcga atttaaagat aatatcgcca actattttgg
    14761 tcagtggccg gtggattata agacctgggc atggagcgaa gatgccgcag tcatggagaa
    14821 gtttaatatc ccccgtcata tgctgtttga tgtgcaaatg ccaggcactg ttcttgggca
    14881 tatcacacct caagccgcgc tggcgacgca tttcccggca ggactgccgg ttgtttgtac
    14941 caccagtgat aaaccggtag aagctctggg agccggatta ctggatgatg aaactgcggt
    15001 aatttctctt ggcacttaca tcgcattgat gatgaacggc aaagccttgc cgaaagatcc
    15061 ggtggcgtac tggccgatta tgtcttctat tccgcaaaca ttgctgtatg aaggttacgg
    15121 tattcgcaaa ggcatgtgga cggtgagctg gctgcgcgac atgttaggcg agtcgttaat
    15181 tcaggatgcc agggcgcagg atctttcacc ggaagattta ctcaacaaga aagcctctag
    15241 tgtgccacct ggctgtaatg ggctgatgac ggtgctggac tggctgacca atccgtggga
    15301 accgtacaaa cgcgggatta tgatcggctt tgattccagc atggattacg catggatata
    15361 tcgttcgata ttggaaagcg tggcgctgac gctgaagaac aattacgaca atatgtgtaa
    15421 tgaaatgaat cactttgcga aacatgtaat cattactggc ggcggttcga acagcgatct
    15481 gtttatgcag atttttgccg acgtgttcaa ccttccggca cgccgtaacg ccattaacgg
    15541 ttgtgcaagc ctgggggcag cgattaatac aggggtaggt ctggggctat acccggatta
    15601 cgcaacggct gtcgataaaa tggttcgcgt gaaagatatc tttataccga ttgagagcaa
    15661 tgccaaacgc tacgacgcga tgaataaagg cattttcaaa gacctaacca aacatactga
    15721 tgtgatcctg aaaaaatcgt atgaagtgat gcatggggaa ttggggaatt ggggaatgcg
    15781 gattcgatcc agagctggtc gaatgcgtga ataaggtagg ccgggcggaa aactgcccgg
    15841 cctgaacata cctgaatggt tatccccgct ggcgcgggga acataagttc gacaacttaa
    15901 aaaatgataa caagtccggt ttatccccgc tgatgcaggg aacataataa aaactttaac
    15961 tagttcggtt tatccctgtt tttacaagga a
//
LOCUS       DAZPDU010000095        15966 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZPDU010000095 DAZPDU010000000
VERSION     DAZPDU010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15966
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.096"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..453)
                     /gene="spy"
                     /locus_tag="ACHURS_002706"
     CDS             complement(<1..453)
                     /gene="spy"
                     /locus_tag="ACHURS_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFR1238393.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPA"
     gene            complement(783..1751)
                     /gene="astE"
                     /locus_tag="ACHURS_002707"
     CDS             complement(783..1751)
                     /gene="astE"
                     /locus_tag="ACHURS_002707"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFR1238394.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGDPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(1744..3087)
                     /gene="astB"
                     /locus_tag="ACHURS_002708"
     CDS             complement(1744..3087)
                     /gene="astB"
                     /locus_tag="ACHURS_002708"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFR1238395.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(3084..4562)
                     /gene="astD"
                     /locus_tag="ACHURS_002709"
                     /pseudo
     CDS             complement(3084..4562)
                     /gene="astD"
                     /locus_tag="ACHURS_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
     gene            complement(4559..5593)
                     /gene="astA"
                     /locus_tag="ACHURS_002710"
     CDS             complement(4559..5593)
                     /gene="astA"
                     /locus_tag="ACHURS_002710"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFR1238396.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRVDFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(5590..6810)
                     /gene="astC"
                     /locus_tag="ACHURS_002711"
     CDS             complement(5590..6810)
                     /gene="astC"
                     /locus_tag="ACHURS_002711"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFR1238397.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDATCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLTTEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            7256..8062
                     /gene="xthA"
                     /locus_tag="ACHURS_002712"
     CDS             7256..8062
                     /gene="xthA"
                     /locus_tag="ACHURS_002712"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFR1238398.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            8229..8944
                     /locus_tag="ACHURS_002713"
                     /pseudo
     CDS             8229..8944
                     /locus_tag="ACHURS_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
     gene            8949..9626
                     /gene="ydjY"
                     /locus_tag="ACHURS_002714"
     CDS             8949..9626
                     /gene="ydjY"
                     /locus_tag="ACHURS_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFR1238399.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            9641..10348
                     /locus_tag="ACHURS_002715"
     CDS             9641..10348
                     /locus_tag="ACHURS_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFR1238400.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAADLTSIRFRSFFIATGLGQLPTTIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            10348..10896
                     /locus_tag="ACHURS_002716"
     CDS             10348..10896
                     /locus_tag="ACHURS_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFR1238401.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSSDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            10906..12072
                     /locus_tag="ACHURS_002717"
     CDS             10906..12072
                     /locus_tag="ACHURS_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFR1238402.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPVV
                     WGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYGTH"
     gene            12045..13581
                     /locus_tag="ACHURS_002718"
                     /pseudo
     CDS             12045..13581
                     /locus_tag="ACHURS_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter permease"
     gene            13581..14234
                     /locus_tag="ACHURS_002719"
     CDS             13581..14234
                     /locus_tag="ACHURS_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR1238403.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALL
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            14301..15608
                     /gene="ynjE"
                     /locus_tag="ACHURS_002720"
     CDS             14301..15608
                     /gene="ynjE"
                     /locus_tag="ACHURS_002720"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFR1238404.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(15617..>15966)
                     /locus_tag="ACHURS_002721"
     CDS             complement(15617..>15966)
                     /locus_tag="ACHURS_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFR1238405.1"
                     /translation="FYALVPFGFILAAPEQNALAGGWLLFAFIGTGSSFLAFAALAAK
                     HQIDNPGYAHKSFYYLGGLTEGTETILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRV
                     WSGYLTLKSLQRQ"
BASE COUNT         3701 a         4345 c         4166 g         3754 t
ORIGIN      
        1 cgctggacgt tctgtcagac gcttctcaaa attagcatta aattgctttt tctgttccgg
       61 cgtcaggatg ttgtaaattt tgttctgggt ttccatgtgc gccagcatgt tagctttgcg
      121 ctgttcttcc atttttgcga tctgcgcttc agcttttgct ttatcgaagg tatcgctggc
      181 aatgatgtca tgcattgcgc ggcgttcttc cagcggcgga cgtttcatct ggtcacgctg
      241 gcctttcatg atttcgcgga tctgctgttt ctgcgcgtcg gtcaggttca ggtctttgaa
      301 catcatgtcc tgatgcggac cgaacttgcc tttgtggtgc atcatcggct tcgcgtcagc
      361 cggtgctgcg gtagtggtgt ctgcggcatg ggccaggtta gccgcgccaa gagccagggt
      421 agaggcaaca aacagtgcag ttaatttacg catattctat atccttcctt tcagttattt
      481 attacggctt tcttaagtag cgtgccgtgt tgacgagatt aactttactg actttagcgt
      541 caattaatca gagcaacggt aaaacaatga aagtgtaaaa aacacttttg cgccaattat
      601 ggagaaaaaa agaaaatttg atggagagtg atgagagaat attacaacac aatgattttg
      661 cagagattat gaagaactat accggatgac tggtgataaa taaagcaaat aaccaggatt
      721 aatctgtatt aatttataag aaagcaactt aatacccgca gaatgatttc tgcgggtaag
      781 tattagctta ttttttcgag cattaatccc gcgcgtaatc ccaacgctac caacggatta
      841 gggaataaca catactctac atcatgggtt acggtaaaac gttcctctcc gtcctgcgcc
      901 agcaatgttc ctttctcaaa cggcataaaa ttcagcgtgt cacttgccat atgcatttcg
      961 aaggacggcg agtggcgagt aatttgcgaa accacccgat aacggagcgg cggtgttctc
     1021 acgataccga cactctcacc agatagcagc gcagcaattg cgctggcagt tactgcaaac
     1081 tggcgaagat cgttttgccc aaagggcaac gctttgccaa gttccagcgt acaggccagc
     1141 gcgccaaaat gtctggcgct gaaatgggta aacgtaccac caggctcctg atggaacacc
     1201 agcgcctcca gccccgccgc acccagccac gtcagaaatt tctcgtccca gggaatgtcg
     1261 cgttgcggta atacaccgaa ctgaggatgc aaggagccac gaattgcggt atgtagatca
     1321 aggtgccaac gcacagattc tttgccctgg tcataaaaat cttccaggca ctgttccagt
     1381 tcgcgcgccc gacaggtttc tccgctttca gcaaatagct gccaacgacc gccaaacatt
     1441 cggttcatat cgctatggca ataacgtttc ccttgcttca gcgcaggagg atccccgagg
     1501 atcaccagca accgccaacg taacgggatc tcgccgtgag atatcgcgcc aagcaacgcg
     1561 tccagcattt ccacaggtgc cgtctcatta ccgtggatcc ccgcagaaat caccagcgcg
     1621 ccttgcggtg gcgttaatgg cgtcagttcc agcacaccat cgcccagcca gcgccagcga
     1681 acgccgttga tttctcgctc ggtgataacc ggttttttac ccgttaaggt cagagcaaga
     1741 aaattatcca ttgccccctc cctcgcgctg gaacggataa accgaaccga gattcagtaa
     1801 ttggctcaat acatccagtg cttcccgccc ttcgcgcagc aattgcgggt cggccagatc
     1861 ggcagcagta aggcgatcgc ggtagtaacg atccacccag tcattgagcg cattaaacag
     1921 cgtatcgttc atcatcaccg ccggattcac cgcccggcgt tcttcttctg tcaatactac
     1981 ccgcaaccgc aggcacgccg ggccgccgcc attcgccatg ctttcacgta aatcaaagac
     2041 ttttagttcg ctaatcgggt tgtcagcggc aaggagttca ttgagataac cccatactcc
     2101 ggcgtgttcc cgacactcct gaggcagcac caacatcatg gaaccatcat cgcggctcaa
     2161 cagttggctg ttaaacagat aggtagacac cgcatccgac acggaaacct gagttgccgg
     2221 aacttctatc gccataaaac cgttcacccg cgcccgcagg tttgccagta actgtgactg
     2281 gcgagcgaac gcctgttggt ggcaaaacag cacctggcgg ttacttacgg caatcacgtc
     2341 attatgaaaa acaccctggt cgataacgtc cgggttttgc tgggcgaaaa tcacctgttg
     2401 gggattcacc tgattaagcc ttgccaccgc ctcgctggct tcgcgagtct gtcgcgccgg
     2461 ataacgggaa ggccgggtat cattgccttc ttctcgcccg tagacaaaaa gttgcatacc
     2521 cggctcaccg taatgaccgc cgagacgatt gtggtttgcc gccccctcat caccgagcaa
     2581 cgctacctgc ggcaacgccg aatggacgct aaatttctct tcgtcgttaa aaatagcttt
     2641 taacagcgat tcagtgacgg gcgcttccag cgaacggtga aatttattgt tcaggttggc
     2701 aacggtgaga tgcactttgc catccagcgt atcggcagat ggcgcgatcg ttgccgcatt
     2761 ggctacccac attggcgaag cggagctgac gctggaaagc cagtgcggtg cctggcgtgc
     2821 aactttttcc agtacctgct catcgctacc actgaatccc aactgacgca gcaccggaat
     2881 aaacggacgc tcgtgcggcg ggatcacggc ctgggggaat cccgcatcgg caagggtttt
     2941 cattttcagt aagccctgtt tcgccgccag tcgcgggtta gacacctgaa aacggtgacg
     3001 ggtagaggct tcattaccaa acgacaggcc agcgtaatga tgcgtcagcc ctaccagccc
     3061 gtcgaaatta acttcccagg cgttcatcgc accacctcat cggaaaaatc cagcccgggg
     3121 ttaagcgttg cgggcaaagt taacgagtcc gactccaggc tcgccatagg ccatgcgcag
     3181 taatctgcgg cataccaggc gctggggcga tggttaccgg atgcaccaat gccgccgaat
     3241 ggcgcggtac tggcagcacc agtaagcggt ttgttccagt taacaatccc cacccgcgcc
     3301 tccagcaaca gttgatcgaa tttttcccgc tcgggggaaa ccagaccgca agagagtccg
     3361 aagcgagtgt tattcgccat tagaatcgct tcctcgaaag aatcataacg ccagacgcgc
     3421 aataacggtc cgaacacctc ttcatctggt acgccagcaa cgcctgtcat ttcaatgatc
     3481 cccggcgtca gcaacgatgt ctctgattgt aataagcgcg gcgcaagcag ggttcgtccg
     3541 cccatcgctt ccagttgctg ctaggcagta accacctgct gtgcggcctg ttcagaaatc
     3601 agcccgccaa taaacggctg cggttcgtca tcccagttgc ccggcgttaa tcgctggctg
     3661 acggcaacca gacgagcaag aaacgcatcg ccctgtgctc cgcttttgag caataaacgg
     3721 cgggcgcagg tgcagcgttg cccggctgtg acaaacgccg actgaatggt cagatggaca
     3781 gccgagtcga tatccgccac ctcatcgata attagcggat tattaccgcc catctcaagg
     3841 gcgagaattt tctccggctg accggagagc tggcgatgca gctggtagcc ggtattggcg
     3901 ctaccggtaa acagcaaacc gtcgagatcc tccagcgcac tcagcgcctg acccgtttca
     3961 cgcccgccct gcaccaggtt cagcacgccc ggcggcaagc cagcctgctg ccataaacgc
     4021 attaccgctt cgccactcca cggtgtcagt tcgctgggtt taaagataat ggtgttacct
     4081 gccagcaatg ccggaacgat atgtccgttc ggcaaatgac cagggaaatt atacggccca
     4141 aacaccgcca gcacgccgtg cgggcgatgt cgcaggctcg ccgcaccgtc cggcatttca
     4201 ctacgctgct cgccggtacg aacgtgatac gccttaattg atatcgcgat tttattgatc
     4261 atcgccgtca cttcggttgc cgcttcccag cgcggcttac ccgtttctct ggcaataatc
     4321 gcggttaatt cggctttatt gctttccagc aatccggcaa agcgttcgac aacgacctga
     4381 cgttcagcca atgagagccg cgcccagcgc ggaaacgccg cacgggctgc ccgacaagcc
     4441 tgttcgacct gagcggcatc ggcatcattg ccttgccata acacctcgcc cgataccgga
     4501 ttacgcttca cacgcgatgc gccctggccc gttatccagt caccgttaat ccataaagtc
     4561 atgctgtttt ctcctctgcg cacaggcgca ccagacgaac gcgatcccct gcgtggcatt
     4621 tgagggcatc cagttgtgcg gcggttaaaa tcaaacgctc ggttgccgga tcggtacgcg
     4681 ccagcaccac gcggaaatgg tgataatttt cattggcgac caggcaggcc ggaaaatcgc
     4741 cctgcgcagg ctgcccttct gccacttcca ccagccgact tttacggatg gcgcgcacgc
     4801 ggtcgatgtc acactcaagc gttggcccgc cgtcgaagat gtcgatatag ttgcggtaac
     4861 gaaaaccttc tttctccagc accgcgcggg caggcgcggt ttgcggatgt acctgaccga
     4921 tgacgtcctg ggcttcctgg gataaaaagt gggtatagat cggatgtttc ggcatcagtt
     4981 ctgcaataaa tgccttttgc ccggtgccgc agagaaaatc gacgcggcta aaatccatcg
     5041 aaaagaagcg tttaccgagg ctttgccaga acggtgaata gccgtgttcg tcaatcaccc
     5101 cgcgcatttc agcaaccact ttgtcattaa acttgtcgcg aaaagccgcc ataaacataa
     5161 agcgcgattt cgacagcaaa tagccgttgc cctctttgcg ccagtccggg tcgagaaaca
     5221 gcgtgcacag ctcgctgctg ccggtgtgat cgttactaag aaacagcgtc ggcaatgcgt
     5281 tatagacatt cagctctttt gaggcgtgaa ccaacgtgcc gacccgatag ttgtaccagg
     5341 gatcgttcag cccaaccgcc acctcaatgg cgcaaatccc cgccacggtg cctgtctcgc
     5401 tatcttccag cacgaacaca tagccctgct cgcttttggg cagttcgcct tgccaggttt
     5461 tgattgccct ttcgatacgc gcggaaagcg tggcttcatt ggcgggaagc gacgtcaggc
     5521 cgccgcccgt tttgctggca agctgcatca gcgccgagac atctgagcgc tcaacgggac
     5581 ggatgaccat catgatgaac ctcggctaac aaagtgttcg caagcagctg caaagcgatc
     5641 cagtccggtc gtcacctctt cttcgctgac attgagcgca ggcgcaaaac gcaccacgtt
     5701 gccacccgca atcagtacca taacgcctgc tttcgccgcc tcctgagaga tctgtttcgc
     5761 ttgtccggcg taatcggcat tcagtacaca gccaatcaac aaacctaagc cgcgaacttc
     5821 actgaacaaa ccgtagcggt gattaatggt attaagacgc tcaacaaacc agtcgtgacg
     5881 ctgtttaacg ccattaagca tctctggtgt gttgatgagc tccagcactt tgcccgccac
     5941 cgccgaggcc agcggattgc cgccataggt ggtgccatga gtgccaacgg tcatcacgct
     6001 ggcgcactct tcggttgtta acaacgcacc gaccgggaaa ccgccgccca gcgctttggc
     6061 ggtagttaac agatcaggtg tcacgccgta gtgcatatag gcatacagtt ccccggtgcg
     6121 cccgacgccg gtttgtactt catcaaaaat caacagcgca ttgtggcggt cacacaattc
     6181 acgcagacct tgtaaaaacg cgttgctggc tggcaccaca ccgccttccc cctggatggg
     6241 ttcgacaatc accgcacagg tggcgtcgtc aatcagcgcg ctggcagagt taatatcgtt
     6301 atatgcagca tgacggatat ctggtggcag tggcgcaaaa tcctgtgaat aggctggctg
     6361 cccacccgca ctgacagtaa acagcgtgcg accatgaaac gcatttttaa acgccacgat
     6421 accgctctta tggctgccgt agcggtcgtg agcgaattta cgcgccagtt ttagcgccgc
     6481 ttcgttggct tccgcaccgg agttacaaaa gaagacgcga tcggcaaacg tggcgtcgat
     6541 caattttttc gccagtcgca gtaccggctc gttggtgtaa ccgttgccgg tatgccagaa
     6601 cttactcgcc tgttcgttca gcgcttcacg cagttccgga tgcgcatggc ccagcgcgtt
     6661 caccgcaatg ccacccgcga agtcgatata ctctttcccc tgctgatccc acaagcgcga
     6721 accttcgcca cgtaccggta taaagggtgc cggagcgtaa acaggtatca tccattcatc
     6781 aaagttttca cgcgtaattg gctgagacat agcgacctct acagtaaata attcgctatc
     6841 tatatgttaa caataagtaa tgtttgcgct gtaaatgtag attgcagggt tcgtgccagc
     6901 cagtgataaa agtgcataaa cggcggaggc taactggaaa tcaaggagtt ataaccaaac
     6961 catatgcatt taaagtgcat ataaagtgaa tacgtttgcg atgtgggtga ataaaaagaa
     7021 taaaaaacgc aatgttatgc agaagtaaaa tataattctg gaattgtgat cattgacgaa
     7081 atttactgga aattactgcg ccattctgac gcagcgcgca ccaaaagcgg gcattttttg
     7141 cgccatcgtt gacatcatta acaaccatcg atcaaatcac ttaacaacag gcggtaagca
     7201 acgcgaaatt ctgctaccat ccacgcactc tttatctgaa taaatggcag cgactatgaa
     7261 atttgtctct tttaatatca acggcctgcg cgccagacct caccagcttg aagccatcgt
     7321 cgaaaagcac caaccggatg tgattggcct gcaggagaca aaagttcatg acgatatgtt
     7381 tccgctcgaa gaggtggcga agctcggcta caacgtgttt tatcacgggc agaaaggcca
     7441 ttatggcgtg gcgctgctga ccaaagagac gccgattgcc gtgcgtcgcg gctttcccgg
     7501 tgacgacgaa gaggcgcagc ggcggattat tatggcggaa atcccctcac tgctgggtaa
     7561 tgtcaccgtg atcaacggtt acttcccgca gggtgaaagc cgcgaccatc cgataaaatt
     7621 cccggcaaaa gcgcagtttt atcagaatct gcaaaactac ctggaaaccg aactcaaacg
     7681 tgataatccg gtactgatta tgggcgatat gaatatcagc cctacagatc tggatatcgg
     7741 cattggcgaa gaaaaccgta agcgctggct gcgtaccggt aaatgctctt tcctgccgga
     7801 agagcgcgaa tggatggaca ggctgatgag ctgggggttg gtcgatacct tccgccatgc
     7861 taatccgcaa acagcagatc gtttctcatg gtttgattac cgctcaaaag gttttgacga
     7921 taaccgtggt ctgcgcatcg acctgctgct cgccagccaa ccgctggcag aatgttgcgt
     7981 agaaaccggc atcgactatg aaattcgcag catggaaaaa ccgtccgatc acgcccccgt
     8041 ctgggcgacc ttccgccgct aatttagcag ctctcctggc tcaaactggg tcaggagaat
     8101 taaccttgag aaaaatcaac aaactgtcag taatgatttg ttgcctgccg tcctttgtta
     8161 taccgtcact gcgtttttag ttgtctgacc acttctctat tatcaagttt gatataggaa
     8221 actccacgat gaatgctgag cgtaaatttc ttttcgcctg tcttattttt gcgctggtca
     8281 tttacgctat ccacgctttc ggtttattcg atctgctcac cgatttaccc cacttacaga
     8341 cactcattcg ccagagcgga ttgttcggtt atagcctcta tattctgtta ttcatcattg
     8401 ccaccctctt tgtgttacca ggaagcatac tggtgatcgc tggaggaata gtttttggcc
     8461 cgctcttagg gacactactc tcattaattg ccgccacgct ggcctcctcg tgctcattcc
     8521 tgctggcgcg gtggctggga cgtgatttac tgctgaaata cgtgggccat agccatacct
     8581 tccaggccat tgaaaaaggc attgcgcgta acggaacggt attgattttc ttattctgac
     8641 ccgcttaatc ccgttgtttc cttacaatat tcaaaattac gcttacggat taaccaccat
     8701 cgccttctgg ccttataccc ttatttcggc actcacgacc ctgcccggta ttgttattta
     8761 taccgtgatg gcaagcgatc tcgccaatga aggcattacg ctgcgcttta ttttacaact
     8821 ctgtctggcg ggcctggcgc tgtttattct cgtccagctc gcaaaactct acgcccgaca
     8881 caaacatgtg gatctgtctg cttcgcgccg cagcccgctt actcacccaa aaaatgaagg
     8941 atagaacgat gtcgcaacat tattcagtgt catggaaaaa aggactggct gcactctgct
     9001 tactggctgt tgccgggctt agcggctgcg atcaaaaaga gaatgcggcg gcaaaagtgg
     9061 aatacgacgg actttcgaac agccaaccgc tgcgcgtcga tgccaataac catacggtaa
     9121 ccatgttggt gcaaattaat ggtcgtttcc tcaccgacga cactcgtcac ggtattgtgt
     9181 ttaaagatgg ctccaacgga cataaatcgc tgtttatggc ttatgcgacc ccgaaagcat
     9241 tttatgaagc cctgaaagag gcaggtggta cgccgggcga aaacatgacg atggataata
     9301 aagaaacgac tcatgtcaca ggcagcaaac tggatatttc ggtcaactgg caaggggcgg
     9361 caaaagcgta ttccttcgat gaagtgattg ttgacagtaa tggcaagaaa ctggacatgc
     9421 gctttggcgg taatttaacg gcagcagaag agaagaaaac gggttgcctg gtgtgtctgg
     9481 atagctgccc ggtcggcatc gtcagcaatg caacatacac ttatggtgcg gtggaaaaac
     9541 gcggcgaagt gaaattcaaa ggcaatgcct cagttctccc ggcggataac acgctggcaa
     9601 cggttacctt taaaatcacc gaataaagcc aggataaagg atgatgaaga tgcaatcgcg
     9661 taaaatctgg tactaccgta taaccctcat catcctgttg ttcgctatgc tgctcgcatg
     9721 ggcgctgctt cccggcgtcc atgagtttat caatcgcagc gttgcggcgt ttgccgccgt
     9781 ggaccaacag ggtatagaac gctttattca gtcttacggc gcactggcgg cagttgtctc
     9841 gttcttgttg atgattttac aggccattgc tgcaccgcta cctgcgtttt tgatcacctt
     9901 tgccaatgcg tcgctgtttg gcgcgttctg gggcggcttg ctgtcgtgga ccagttcgat
     9961 ggccggcgcg gcgctgtgct tttttatcgc cagagtgatg ggccgcgaag tggtggaaaa
    10021 attaaccggc aaaaccgtgc ttgacagtat ggacggcttt ttcactcgct acggcaaaca
    10081 caccatcctg gtctgtcggt tattgccttt tgtccctttc gatccaatca gctatgctgc
    10141 cgatctgact tcaatacgtt ttcgctcgtt ttttatcgcc accgggcttg gtcaattacc
    10201 gacgactatt gtttattcct gggcgggcag catgttaaca ggcggtactt tctggtttgt
    10261 caccggactg tttattctgt ttgccctgac cgtggtgatt tttatggcga agaaaatatg
    10321 gcttgagcgc cagaagagga atgactgatg ggtttaccgc cgcttagcaa aattcctttc
    10381 attttacgtc cgcaggcgtg gctgcatcgt cgccattacg gcgaggtgct aagcccgatt
    10441 cgctggtggg ggcggatccc gtttatcttt tatttggtgt caatgtttgt tgggtggctg
    10501 gagcgcaaac gctcaccgct cgatccggta gtgcgatcgc ttgtcagcgc gcgcattgcg
    10561 caaatgtgcc tgtgtgagtt ttgtgtggat atcaccagta tgaaagtcgc cgagcgtacc
    10621 ggcagcagcg ataaactgct ggcagtggct gactggcggc aaagcccgct ctttagcgat
    10681 gaagaacggc tggcgctgga gtacgccgaa gccgcaagcg tcacaccgcc aacagtcgat
    10741 gatgccctgc gtacccgact ggctgcgcat tttgacgctc aggcgctcac cgaactgacg
    10801 gcattgatcg gcctgcaaaa cctgtcggct cgttttaatt ctgccatgga cattcccgct
    10861 caggggctgt gccgtattcc ggaaaaacgt tcttaaggag agaagatgcg ccattgtggg
    10921 tggttgctgg gattgttatc gctgttttct ttggcaacac atgccagtga ctggcaggaa
    10981 attaaaaatg aggccaaagg gcaaaccgtc tggtttaacg cctggggcgg cgataccgca
    11041 attaaccgct atctcgactg ggttagcggc gagatgaaaa cccattacgc tataaacctg
    11101 aagattgttc gtctcgcgga tgccgcagac gcggtgaagc gcattcagac cgaagctgct
    11161 gccggtcgta aaacgggcgg ctcggtggat ctgctctggg tgaacggcga aaacttccgc
    11221 accttaaaag aggccaattt attacaaacg ggctgggcgg agactctgcc caactggcgc
    11281 tatgtcgaca cacagctgcc ggtgcgggaa gatttttcag tgccgacaca aggtgcggaa
    11341 tcgccctggg gcggcgcaca actgacgttt atcgcccgcc gcgatgttac gccacagcca
    11401 ccacaaacgc cgcaagcctt actggagttt gctaaagcca atcccggcac ggttacttat
    11461 ccgcgcccac cggactttac cggcacggcg tttcttgaac agttgctgat tatgctgacg
    11521 ccagatcccg ccgcattaaa agaagcgccg gacgatgcga ctttcgcccg tgtcactgcg
    11581 cccttgtggc aatatcttga tgcactgcat ccgtatttgt ggcgcgaagg aaaggatttc
    11641 ccgccttcgc ccgcgcggat ggatgctctg ctgaaagccg gcacattgcg cctgtcgctg
    11701 acctttaacc ccgcgcatgc gcagcaaaaa atcgccagcg gcgatttgcc ggcaagcagt
    11761 tacagttttg gctttcgcga ggggatgatt ggcaacgtgc atttcgtcac cattccagcc
    11821 aacgcgaatg ccagtgctgc ggcgaaggta gttgccaatt tcctgctctc acccgatgcg
    11881 caactgcgta aagcagatcc cgttgtctgg ggcgatcctt ctgttctcga tccgcaaaaa
    11941 ctgcctgacg ggcagcgcga aatattgcaa tcaagaatgc cgcaggatct gccgccggta
    12001 ctggctgaac cgcacgcagg atgggtgaat gcactggaac aagaatggtt acgccgttac
    12061 ggtacgcatt aatctttttg ctgtgggcga tggtggcggt gatttatgca ccgctgatcc
    12121 cggcagctct cacgctgata tcgcctgcgt tgtcgttgac acactggcag gcgttatttg
    12181 ccgatccaca gttaccgcat gcattactgg caacgctggt atcgacaacc atcgcggcgg
    12241 tcggggcatt gttgattgcc ctgctggtga ttgtggcgct atggcctggg ccgaaatggc
    12301 agcgtatgtg cgcccgtctg ccgtggctgc tcgccattcc ccatgtggct tttgccacca
    12361 gcgccctgct gctctttgct gacggagggc tgctatatga ctatttcccg tatttcactc
    12421 cgccaatgga ccgatttggc atcggtctgg gcctcaccct tgcagtgaaa gaaagcgcat
    12481 ttctgctgtg gatcttagcg gcagtattga gcgaaaaacg gctgttgcag caggtcattg
    12541 tgctggattc gctgggctac agccgctggc aatgcctgaa ctggctgctg ttgccctccg
    12601 tcgcgccttc gctggcaatg gcgatgctgg cgattgttgc ctggtcgctt tcggtcgtgg
    12661 atgtggcgat tattcttggg ccaggtaatc caccgacgct ggcagtaatt agctggcagt
    12721 ggttaactca gggcgacgcg gatcaacaaa cgaaaggcgc actcgccagt ctgctgctga
    12781 tgctgttact cgccgcctac gttttgctga gctatctgct atggcgcagc tggcggcgca
    12841 ctattccccg cgtagatggc attcgcaatg cctgccacac ctttattacc aggcactacg
    12901 ctggcgagtt ttttaccctt aaccggtgtg ctgtgtgtgg ttctgctggc gatcctcgcg
    12961 gatcagtcga cgatcaatag tgaagcactc atcaacagtc tgacaatggg gctggtagca
    13021 acattcatcg ctttgctcct gttactgctg tggctggaat gggggccaca gcgtcgccag
    13081 ttgtggctat ggttgcccat tctattgccc gatctgccac tggtggcggg ccagtacacg
    13141 ctggcgctat ggctgaacct agatggaagc tggacggcgg tggtctgggg gcatctgctg
    13201 tgggtaatgc cgtggatgct gtttatcctg caacccgcct ggcagcgcat tgattcacgg
    13261 ttaattttga ttgcgcaaac actgggctgg tcgcgggcca aaatcttctt ttacgtgaaa
    13321 tgcccactca tgttgcgccc tgtgctgatt gccttcgcgg tgggattttc agtcagtatt
    13381 gcgcagtata tgccgacgct gtggctgggc gcggggcgtt ttccgacgct caccactgag
    13441 gcggtggcat taagcagcgg cggcagcaac ggtattctcg ccgcccaggc tttatggcaa
    13501 ctgttgttac cgcttattat ttttgccctg accgcgttag tcgcaaaatg ggtaggttat
    13561 gtcagacaag gactccgcta atgctctgcg tgaaaaatgt ttcgctacgt ttaccagaaa
    13621 gccgcttgct gacaaacgtt aactttacgg ttgataaagg tgacatcgtc acgttaatgg
    13681 ggccgtctgg ctgtggaaaa tccactctgt tttcatggat gattggtgca ctggctggac
    13741 agttttcttg tacaggtgag ctatggctca atgagcaacg gattgacatg ctacccaccg
    13801 cacagcgtca gattggcatt cttttccagg atgcactgtt atttgaccag ttcagcgtcg
    13861 ggcaaaattt actgctggcg ctactgtcga cgctcaaggg gactgccagg cgcaatgccg
    13921 tgaaagacgc gcttgatcga gcgggactgg ctgaaactta tcaccaggac cctgcgacat
    13981 tatcaggcgg tcagcgagcg cgggtggccc tgctgcgcgc ccttctcgct cagccaaaag
    14041 cgttactcct ggatgagcca ttcagccgtc ttgatgtggc tctgcgcgat aattttcgcc
    14101 agtgggtgtt cagcgaagtt cgcgaactgg cgatccccgt cgttcaggta acgcacgatc
    14161 tccaggatgt tcctgctgat agttctgttc tggatatggc gcagtggtca gaaaattaca
    14221 acaaactgcg ataacgcaaa gtttttctca atgcgtcagt tcagaatggc gcactcaaaa
    14281 ctacaatgtc gggattttcg atgaaacgtg tttctcaaat gaccgcgctg gcaatggctt
    14341 tagggctggc ttgcgcttct tcgtgggccg ctgaactggc gaagcctctt acacttgacc
    14401 agcttcaaca acaaaatggc aaagcgatag atactcgccc cagcgcgttt tataacggct
    14461 ggccacaaac cttaaatggc ccttctggtc atgaacctgc cgccttaaac ctctctgcca
    14521 gctggctcga caaaatgagc accgaacagc tcaacgcgtg gatcaagcaa cataacctga
    14581 aagccgatgc cccggtggcg ctgtacggta atgacaaaga tgtcgacgcc gtcaaaacgc
    14641 gactgcaaaa agcaggtttt acgcatatct ccattctgag tgacgcgcta agcgaacctt
    14701 cccgtctaca aaaactgccg cactttgagc agctggttta tccgcaatgg ctgcacgacc
    14761 tgcaacaagg taaagaggtt acggcgaaac ctgccggtga ctggaaagtc attgaagcgg
    14821 cctggggcgc tcctaagctt taccttatca gccatattcc cggtgctgac tacatcgata
    14881 ccaacgaagt ggaaagcgaa ccgctgtgga acaaagtttc tgatgaacaa ctaaaagcga
    14941 tgctggcaaa acacggcatt cgccatgaca ccacggtcat tctgtacggg cgtgacgtat
    15001 acgctggagc gcgtgtggcg caaattatgc tctacgcagg cgtgaaagat gtgcgtctgc
    15061 tggatggcgg ctggcaaacc tggtccgacg cgggactgcc tgttgagcga ggaacgccac
    15121 cgaaagtgaa agcggaaccg gatttcggcg tgaagatccc ggcacaaccg cagttgatgc
    15181 tcgatatgga acaggcccgt ggactgctgc atcgccagga tgcatcgctg gtgagcattc
    15241 gttcgtggcc agaatttatc ggtacgacca gcggttacag ctatattaaa ccaaaaggtg
    15301 aaatagccgg agcacgttgg ggacacgctg gtagcgactc gacgcatatg gaagattttc
    15361 ataacccgga tggcaccatg cgcagcgccg atgatattac cgctatgtgg aaagcgtgga
    15421 atatcaaacc agagcagcaa gtttcattct actgcggcac cggctggcgc gcgtccgaaa
    15481 cctttatgta cgcacgcgcc atgggttgga agaatgtttc cgtgtatgac ggcggctggt
    15541 acgaatggag cagcgatcca aaaaatccgg tagcaaccgg tgaacgcggc ccggacagta
    15601 gcaaataaca ttgcaattac tgacgctgga gcgacttcag cgtcagatac ccactccaca
    15661 cccgcgtaaa ggtcgtcatc cagcacaacg caccgaagat ccacgcaaac cacgcaaacc
    15721 atgcagggaa taaacatccc agcacaaaca gtaagatcgt ttctgtgcct tcggttaatc
    15781 cccccagata gtaaaacgac ttatgtgcat aaccggggtt atcaatctga tgttttgctg
    15841 ccagtgcagc aaaggcgaga aaactgctgc ccgtgccgat aaacgcaaac aacaaccagc
    15901 cgcctgccag cgcattttgc tccggtgcag caagaataaa gccaaacggc accagcgcgt
    15961 aaaaga
//
LOCUS       DAZPDU010000096        15885 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZPDU010000096 DAZPDU010000000
VERSION     DAZPDU010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15885
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.097"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..558
                     /gene="traW"
                     /locus_tag="ACHURS_002722"
     CDS             <1..558
                     /gene="traW"
                     /locus_tag="ACHURS_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HFR1238406.1"
                     /translation="DLWPVKEPDMLTVIMQRLTALEQSGEMGRKMDAFKERVIRNSLR
                     PPAVPGIGRTEKYGSRLFDPSVRLAADIRDNEGRVFARQGEVMNPLQYVPFNQTLYFI
                     NGDDPAQVAWMKRQTPPTLESKIILVQGSIPEMQKSLDSRVYFDQNGVLCQRLGIDQV
                     PARVSAVPGDRFLKVEFIPAEEGRK"
     gene            555..1547
                     /gene="traU"
                     /locus_tag="ACHURS_002723"
     CDS             555..1547
                     /gene="traU"
                     /locus_tag="ACHURS_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HFR1238407.1"
                     /translation="MKRSLWLLMLFLLAGHVPAASADSACEGRFVNPITDICWSCIFP
                     LSLGSVKVSQGKVPDTANPSMPIQICPAPPPLFRRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPAFGKTAQGTAKKDEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNMPLDVLFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVMRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            1577..1882
                     /locus_tag="ACHURS_002724"
     CDS             1577..1882
                     /locus_tag="ACHURS_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1238408.1"
                     /translation="MATMDSKYIQHLRKISAVLLRLISRTLKLFPFGLVMVPVILFYF
                     APDSFLAEIIHEWKKADISEQVTLVRLYLKVTFTCAFMAVFLNYLFSDEFMPVNSRK"
     gene            1891..2529
                     /gene="trbC"
                     /locus_tag="ACHURS_002725"
     CDS             1891..2529
                     /gene="trbC"
                     /locus_tag="ACHURS_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HFR1238409.1"
                     /translation="MNRKIFALFALTAGMHLAVHASENVNTPENRQFLKQQENLSRQL
                     REKPDHQLKAWAEKQVLENPLQRSDNHFLDELVRKQQASQDGKPRQGALYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPALVVFCSQGYDIIRGNLRVGQALEKVATTGDCRQVAHDLLAGKGDSGK"
     gene            2526..4376
                     /gene="traN"
                     /locus_tag="ACHURS_002726"
     CDS             2526..4376
                     /gene="traN"
                     /locus_tag="ACHURS_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HFR1238410.1"
                     /translation="MKRILPLILALVAGMAQADSNSDYRAGSDFARQIQGQGTGSIQG
                     FKPQESIPSYNANPDETKYYGGVTAGGDGGLKNDGTTEWATGETGKTITESFMNKPKD
                     ILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQEINRSEFTNYTCERDTMVEEYCTRT
                     ASITGDWKYTDEYREVTIPHSQFRFSMNGLKLVFSVTAPVTGTVESASLSVYAAFFFL
                     NSRYTFMNTTFNVGLANGQSDTYPLSGATGLQVTQGQVLTGSGCTANGNCLPHGNGDR
                     KVYESLVSGASTFTLKLRMKVRDKEWVPRVEWVESCPFNKADGVLTGTECSEPGGTKT
                     GVMEGKPWNITQACWAYRDKYVTQSADNGTCQKYVDNPACTLASRQCAFYSDEGTCLH
                     EYATYSCESKTSGKVMVCGGDVFCLDGECDKAQSGQSNDFAEAVSQLAALAAAGKDVA
                     ALNGVDVRAFTGQAKFCKKAAAGYSNCCKDSGWGQDIGLAKCSSDEKALAKAKSNKLT
                     VSVGEFCSKKVLGVCLQKKRSYCQFDSKLAQIVQQQGRNGQLRIGFGSAKHPDCRGIT
                     VDELQKIQFDRLDFTNFYEDLMNNQKIPDSGVLTQKVKEQIADQLKQAGQ"
     gene            4403..4660
                     /locus_tag="ACHURS_002727"
     CDS             4403..4660
                     /locus_tag="ACHURS_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HFR1238411.1"
                     /translation="MKVIFTSNRFIDFLIRLLITAFVISPVIIWSWDTVKETTAGEML
                     AACFVIFYSGVLLFILYFCFSALTGLQKDDERKNDERNEDE"
     gene            4653..5396
                     /gene="traF"
                     /locus_tag="ACHURS_002728"
     CDS             4653..5396
                     /gene="traF"
                     /locus_tag="ACHURS_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HFR1238412.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAA
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            5410..5751
                     /gene="trbA"
                     /locus_tag="ACHURS_002729"
     CDS             5410..5751
                     /gene="trbA"
                     /locus_tag="ACHURS_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HFR1238413.1"
                     /translation="MHTGDLCFFTGASLFIVGVVGYFFTGPNNKGVYLLSSFVFFVVN
                     VISVCMLPDNFLFLCGAVMNQCAVPVVSMAVSTIPVITKFDSFRRMFVCIMLSAIWSG
                     VMWFFIISLVK"
     gene            complement(5807..6067)
                     /locus_tag="ACHURS_002730"
     CDS             complement(5807..6067)
                     /locus_tag="ACHURS_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin ArtA"
                     /protein_id="HFR1238414.1"
                     /translation="MKKLSFKEKLEIVRNIIRESLSGNAAFALLVYSLLHSLPIKTFA
                     DLFVTILLFIAIIAVVIWLFLIIYVYFCELFRSHWIAVWFIL"
     gene            6148..6432
                     /gene="traQ"
                     /locus_tag="ACHURS_002731"
     CDS             6148..6432
                     /gene="traQ"
                     /locus_tag="ACHURS_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HFR1238415.1"
                     /translation="MISKYRFSLPRLDITGMWVFSLGVWFHIVARLVYSKPWMAFFLA
                     ELIAAILVLFGAYQVLDAWIARVSLEEREALEARQQAMMEGQQEGGHVSH"
     gene            6419..6964
                     /gene="trbB"
                     /locus_tag="ACHURS_002732"
     CDS             6419..6964
                     /gene="trbB"
                     /locus_tag="ACHURS_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HFR1238416.1"
                     /translation="MSLTKLLPVILLMMATGVQASTRDEIERLWNPQGMAVQPAQPAA
                     DTSARTEKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSV
                     FPYTLDGQGDTAFPEALPVPPDVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     ASFMARMDTVLQMYGEEKGAK"
     gene            6954..7268
                     /gene="trbJ"
                     /locus_tag="ACHURS_002733"
     CDS             6954..7268
                     /gene="trbJ"
                     /locus_tag="ACHURS_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HFR1238417.1"
                     /translation="MRNKQVVLLIAGISGIATGIIVSLNIPFIRQGLFYPASPVEIVV
                     SLCLTFSVSVVFFVGAIVGWISVSEIYYSCMACLNESSEISEETYDDRKQKESGIQNQ
                     KN"
     gene            7222..7614
                     /gene="trbF"
                     /locus_tag="ACHURS_002734"
     CDS             7222..7614
                     /gene="trbF"
                     /locus_tag="ACHURS_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HFR1238418.1"
                     /translation="MTENKKNQEFKIRKIKRNIERSCDNAIKYFELFVVFFVAGLLLW
                     HGMHAFFSAGIDSWKADPELNNLIYMWNILMYAIPYTLYALAAGFLVTSFLSPMYELI
                     FGNIMIFLLKRRMRRENKLREGGNNASH"
     gene            7601..8974
                     /gene="traH"
                     /locus_tag="ACHURS_002735"
     CDS             7601..8974
                     /gene="traH"
                     /locus_tag="ACHURS_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraH"
                     /protein_id="HFR1238419.1"
                     /translation="MLRIKPLLVLCAALLTVTPAASADVNSDMNQFFNKLGFASNTTQ
                     PGVWQGQAAGYAYGGSLYARTQVKNIQLISMTLPDINAGCGGIDAYLGSFSFINGDQL
                     QRFVKQIMSNAAGYFFDLALQATVPEIKTAKDFLQKMASDINSMNLSSCQVAQGIVGG
                     LFPQTQVSQQKVCQDIAGESNIFADWAASRQGCTVGGQSSSVRDKASDKDKERVLKNI
                     NIIWDSLSKNRMLDGNKELKEFIMTLTGTLIFGEDSEITPLPARTTDRDILRAIMEGG
                     TAKVYHCNDSDKCLKVVADANVTIARDKALKSQITKLLTSIQNKAVSDTPLDDREKGF
                     ISSTTVPVFKYLIDPQMLGVSSSVVYQLTDYIGYDIMLQYIQELLQQARAMIATGNYP
                     QTVLDSVLENLNQAQQQIAVFQGQVKVQQDALLVVDRQMSYMRQQLSARMLSRYQNNY
                     HFGGSTL"
     gene            8971..11796
                     /gene="traG"
                     /locus_tag="ACHURS_002736"
     CDS             8971..11796
                     /gene="traG"
                     /locus_tag="ACHURS_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating-pair stabilization
                     protein TraG"
                     /protein_id="HFR1238420.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMGTRTWDSIEKIALTLSVLAV
                     AVMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVQVHRVDNVPVGLAMPL
                     SLTTRIGHAMVASYEMIFTQPDSATYSKTGMLFGANLIVKSTDFLSRNPEIINLFQDY
                     VQNCVLGDIYLNHKYTLEDLMASGDPYTLIFSRPSPLRGVYDSNNNFITCKDASVTLK
                     DRLNLDTKTGGKTWHYYVQQIFGGRPDPDLLFRQLVSDSYSYFYGSSQSASHIMRQNV
                     TMNALKEGITSNAARNGDTASLVSLATTSSMEKQRLAHVSIGHVLMRNLPMVQTILVG
                     ITIGIFPLLVLAAVFNILTLSVLRGYVFALMWFQTWPLLYAILNSAMTFYAKQNGAPV
                     VLSELSQIQLKYSDLASTAGYISVMIPPLSWMMVKGLGAGFSSVYSHFASSAISPTAS
                     AAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGNMVMDA
                     SGAMSRLPVGINATRQIAAAQQEMAREASNRAESALHGFSSSIASAWNTLSQFGSNRG
                     SSDSVTGGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATQELASTGSKFSYGGY
                     ADAHAEWGIKPGILGVGGGVGVRGGFKGSLDRTDDDSHSASSGSRATHDARHDIDAKA
                     SKDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEY
                     AEMASRTESMSGQMSEDLSQQFAQYVMKRAPQDAEAILTNTSSPEIAERRRAMAWSFV
                     QEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQAIIEQRTQDSNIRND
                     VKHQVDNMVTEYRGNIGDTQNSIRGEENIVRGQYSELQNHHKTEALSQNNKYNEERES
                     QKIIPEPSEEAVKKMVDDKKDRLKGPL"
     gene            11793..12302
                     /gene="traS"
                     /locus_tag="ACHURS_002737"
     CDS             11793..12302
                     /gene="traS"
                     /locus_tag="ACHURS_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898910.1"
                     /note="TraS, as the term is used in F-like plasmids, is a
                     plasmid entry exclusion protein. It interacts with the
                     F-like plasmid mating stabilization protein TraG; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer entry exclusion protein TraS"
                     /protein_id="HFR1238421.1"
                     /translation="MITHYDIKTETQKLKDVLSVEGVNIPPLLQVIKPGVYVFLWVLL
                     WPTFLRLLADKVDIRDAGFDICFSGVMGFILFVAITNGMMLYLAIPKKFRDESKVISF
                     MYDKNKTYILSFVIVFSIVSFAHTFLFGFLSITLFVIISFIYTIDINRYNLSAIVSVI
                     GLFKKESVS"
     gene            12316..13047
                     /gene="traT"
                     /locus_tag="ACHURS_002738"
     CDS             12316..13047
                     /gene="traT"
                     /locus_tag="ACHURS_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer complement resistance protein
                     TraT"
                     /protein_id="HFR1238422.1"
                     /translation="MKTKKMMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LKPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDAAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSNSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            13250..13987
                     /locus_tag="ACHURS_002739"
     CDS             13250..13987
                     /locus_tag="ACHURS_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1238423.1"
                     /translation="MTSDNIAEYIGENNQFTEKPATEYPPVIQDNTVTTKDYLYSNNI
                     DTDLLVVIGGLIVILFLMMLIMYFINSLRHKELTEILTKISFPEKSGSNLLKPEAVSC
                     NLLSLCVLAPVFCTPRKLAEVSREIIVASAQEIAWTPGSSRIDGHQAENITWAISSQS
                     PADKYDLINKTSCVSPDSGRFYEPDTVLDHRERVRLFIQIQFPHGSDWSEVLRLTDIV
                     TEKVSSGRTAGGHHHRHTGYTWSVSVN"
     gene            14038..>15885
                     /gene="traD"
                     /locus_tag="ACHURS_002740"
     CDS             14038..>15885
                     /gene="traD"
                     /locus_tag="ACHURS_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFR1238424.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILIGLV
                     LWVKISWQTFINGCIYWWCTSLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLASVVALVICLITFFVVSWILGHQGKQQSENEVTGGRQLTDNPKEVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVISMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQARP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPEVASGEDVTQA"
BASE COUNT         4000 a         3630 c         4299 g         3956 t
ORIGIN      
        1 gatctgtggc cggtaaagga gccggacatg ctgacggtga tcatgcagcg cctgacggca
       61 ctggagcagt ccggtgagat gggccggaaa atggatgcct ttaaggagcg ggtgatccgc
      121 aacagtctgc ggccccctgc cgtgcccggt atcggacgca cggagaaata cggcagccgg
      181 ttgtttgacc cgtccgtcag actggctgcg gatatccggg ataacgaagg gcgggtgttt
      241 gcccgtcagg gtgaagtgat gaatccgctg cagtatgtgc cttttaacca gacgctgtat
      301 ttcatcaatg gcgatgatcc ggcgcaggtg gcctggatga aacgccagac gccgcccaca
      361 ctggagagca aaattatcct cgtgcagggc agtatcccgg agatgcagaa gtctctggac
      421 agccgtgttt actttgacca gaacggtgtg ctctgccagc gcctcggcat tgatcaggtc
      481 ccggcacggg tgagcgccgt tcccggcgat cgcttcctga aggtggaatt tattccggca
      541 gaggagggca gaaaatgaag cgaagcctgt ggctgctgat gttattcctt ctcgccggtc
      601 atgtccctgc agcgtctgcg gattctgcct gtgaggggcg ttttgtaaac ccgatcacgg
      661 atatctgctg gagctgtatt ttcccgctct cgctgggcag tgtcaaagtc agtcagggta
      721 aggtccccga cacggcgaac ccgtcgatgc ccattcagat ttgtccggca ccgccgccgc
      781 tgttcaggcg tatcgggctg gccattggtt actgggagcc gatggcgctg acggacgtca
      841 cccggtcacc gggatgcatg gttaacctgg gcttcagcct gccggctttt ggtaaaacgg
      901 cacagggaac ggcgaaaaag gatgagaagc aggtgaacgg ggcgttctat cacgttcact
      961 ggtacaaata cccgctgacg tactggctga acatcatcac atcgctgggc tgtctggaag
     1021 gtggtgacct ggatatcgct tatctttctg aaattgaccc cacctggacg gacagcagcc
     1081 tgaccaccat tctcaatccg gaagctgtca tctttgccaa tccgatagca cagggggcct
     1141 gtgcagcaga tgccattgcc agcgccttta atatgcctct cgatgttctg ttctggtgtg
     1201 ccggttcgca gggaagtatg tacccgttca atggctgggt gagtaatgag tccagtccgt
     1261 tgcagtcctc cctgctggtc agtgaacgca tggcgttcaa actacaccgt cagggcatga
     1321 ttatggaaac catcgggaaa aataacgccg tctgtaatga atatccgtcc ccaatcctgc
     1381 ccaaagaacg ctggcgttat cagatggtga atatgtatcc ggacagcggg cagtgccatc
     1441 cgttcgggcg cagtgtgatg cgctgggaga cgggaaaaaa tccgcccaac acaaagaaaa
     1501 acttcggcta cctgatgtgg cgtaaacgta actgtgtatt cctgtaaatc attttaatga
     1561 ataagaggaa atgaaaatgg caacaatgga cagtaaatat attcagcacc tcaggaaaat
     1621 aagtgctgtg ctgctcaggc tgataagcag aacattaaaa ttatttcctt ttggtcttgt
     1681 aatggtgccc gttatattat tttattttgc acctgacagt tttctcgctg aaattattca
     1741 tgaatggaag aaggcagata tatcagaaca agtgacactt gtcagattat atctgaaggt
     1801 gacatttaca tgtgcgttta tggctgtttt tctcaattat cttttttctg atgagttcat
     1861 gcctgtaaat agcagaaagt gagaaatatc atgaaccgga aaatatttgc actttttgct
     1921 ctgacagcag ggatgcatct tgctgttcat gcttcagaaa atgtgaacac acctgaaaac
     1981 cgccagttcc tgaagcagca ggaaaattta agcagacaac tgcgagagaa acctgaccat
     2041 cagctgaaag cctgggcgga gaaacaggtg ctggaaaacc cccttcagcg ttcagataac
     2101 cattttctgg atgagctggt tcgtaaacag caggcttcgc aggacgggaa accccggcag
     2161 ggtgccctgt attttgtgtc gttttccatt cccgaagagg ggctgaaacg gatgctgggc
     2221 gaaacccggc actacggtat tccggccaca ctgcggggca tggtgaataa tgacctgaag
     2281 accaccgctg aagccgtgtt gtccctggtg aaagacggcg caactgatgg tgttcagatc
     2341 gacccgacgc tgttttcaca gtacggcatt cgcagcgtac cggcactggt ggtgttctgc
     2401 agtcagggat acgacatcat ccggggaaac ctgcgggtcg gccaggcgct ggagaaagtg
     2461 gccaccacgg gtgactgccg gcaggtggct catgatttac tggcagggaa aggagattcc
     2521 gggaaatgaa acgtatttta cctctgatac tggctctggt tgccggcatg gcacaggctg
     2581 acagcaacag tgattaccgg gccggttctg attttgcccg tcagattcag gggcagggta
     2641 caggcagtat tcagggcttt aagccacagg agagtatccc cagctataac gcgaatccgg
     2701 acgagacaaa atattacggt ggcgtgactg ccggcgggga tggcggcctg aaaaatgacg
     2761 gcaccacgga atgggcgacc ggtgaaaccg gaaaaaccat cacagagtcc ttcatgaaca
     2821 agccgaaaga cattctgtca ccggatgcac cgttcattca gaccggcagg gatgtggtga
     2881 acagggctga cagcattgtg ggaaataccg gacagcagtg cagtgcgcag gagataaacc
     2941 gcagtgaatt cacaaactat acctgtgagc gggacacgat ggtggaggag tactgcacac
     3001 ggactgccag tatcaccggg gactggaaat acacggatga atatcgtgaa gtgacgatac
     3061 cgcactcaca gttccggttc agtatgaacg ggctgaaact ggtgttcagt gtcacggctc
     3121 cggtaaccgg tacagtggaa agtgcatcat tgtctgtata cgccgctttt ttcttcctga
     3181 actcccgtta taccttcatg aatacaacct ttaatgtcgg cctggcaaac gggcagtcgg
     3241 atacttaccc cctgtccggg gcgacaggtt tgcaggtgac acaggggcag gttctgaccg
     3301 gtagtggctg tactgctaac ggtaattgcc tcccgcacgg gaatggtgac aggaaagtct
     3361 atgaatcgct tgtcagtggt gcttccacct ttacgctgaa actcaggatg aaggtccgtg
     3421 ataaagaatg ggttccccgg gtggagtggg tggagagttg tccgtttaat aaagcggatg
     3481 gcgtgctgac aggcactgag tgttcagagc cgggtggtac gaaaaccgga gtgatggaag
     3541 gtaaaccctg gaacatcaca caggcatgct gggcataccg ggataaatat gtcactcagt
     3601 ccgcagataa cggcacctgt cagaaatatg tcgataatcc ggcctgtacg ctggcctcac
     3661 gtcagtgcgc gttttattcc gatgagggaa cctgtctgca tgagtacgcc acgtactcct
     3721 gcgagtcaaa aacatccggg aaagtgatgg tctgtggggg agacgtcttc tgtctcgatg
     3781 gcgaatgtga taaggctcag agcgggcaga gtaatgattt tgccgaagcc gtttctcagc
     3841 tggcggcact ggccgcagca ggtaaggatg ttgcggcgct gaacggggtg gatgtccgtg
     3901 ctttcaccgg tcaggcgaaa ttttgtaaga aggctgcagc cggatacagc aactgctgta
     3961 aggacagcgg ctggggacag gatatcgggc tggccaaatg cagcagtgat gaaaaggccc
     4021 tggctaaagc aaaatcaaac aaactgaccg tcagtgtcgg agagttctgt tcgaagaaag
     4081 tgctgggtgt ctgtctgcag aaaaagcgca gctactgcca gtttgattcg aagctggcgc
     4141 agatagtcca gcagcagggg cgtaacgggc agttgcgtat cgggtttggc agtgcgaagc
     4201 atcctgactg tcgggggatt acggttgatg agctgcagaa aattcagttc gacagactgg
     4261 atttcactaa cttctacgaa gacctgatga ataaccagaa aatcccggac agtggcgttc
     4321 tgacgcagaa agtgaaagag cagattgctg accagctgaa acaggcagga cagtaaggta
     4381 tggaaaataa ccgggggaaa tgatgaaagt catattcacg tcgaaccgtt ttattgattt
     4441 tcttatccgg cttttgatta cagcgtttgt catttccccg gtaattatct ggtcgtggga
     4501 tacggtaaaa gaaaccactg caggtgagat gcttgctgct tgttttgtta ttttttactc
     4561 cggtgtgctc ctgtttattc tgtatttttg tttttctgcc ctgactggcc tgcagaaaga
     4621 tgatgaaaga aaaaatgatg aaaggaatga agatgaataa agcattactg ccactgttac
     4681 tctgctgctt tatttttccg gcgtcaggga aagatgcagg ctggcagtgg tataacgaga
     4741 aaataaatcc gaaggaaaaa gaaaataaac ctgtacctgc cgccccccgt caggagccgg
     4801 atattatgca gaaactggcc gcgctgcaga cggcaacaaa gcgggcgctg tacgaagcca
     4861 ttctgtatcc cggcgtggat aattttgtta aatatttccg gctgcagaat tactggactc
     4921 agcaggccgg gcttttcacc atgagtgcta aaaaggccat gctggcacat cccgaactgg
     4981 actataacct gcagtacagc cattacaacg gcacggtacg gaaccagctg gcggcagacc
     5041 aggcgcagca gcgacaggcc attgcgaaac tggctgaaca ctacggcatc atgttttttt
     5101 accgggggca ggaccccatc gacgggcaac tggcgcaggt cattaatggc ttccgggata
     5161 cgtatggtct gagtgttatt cccgtttccg tggatggcgt gattaatccg ctgttgccgg
     5221 attcccggac tgaccagggg caggcgcagc gcctcggtgt gaaatatttc ccggccatga
     5281 tgctggttga cccgaaacag ggcagtgttc gcccgttatc atacggcttt atttcacagg
     5341 acgacctggc aaaacagttc ctgaacgttt ctgaagattt taaaccgaat ttttaaccaa
     5401 aggttatata tgcatacagg ggatttgtgt ttttttacag gtgccagcct gtttatcgtg
     5461 ggggttgttg gttatttctt tacaggacca aataataaag gagtttatct gctgagctcg
     5521 tttgtcttct ttgtagtaaa tgtcataagt gtatgtatgc tgccagataa tttcttgttt
     5581 ctttgtggtg cggttatgaa tcagtgtgcc gtaccggttg tttcaatggc agtcagtacc
     5641 ataccggtta tcacgaagtt tgacagtttc agaaggatgt ttgtttgtat tatgttgtca
     5701 gccatatggt ctggagtgat gtggtttttt attatttcat tagtgaaatg actggtgcta
     5761 atttgtcata aatgccaaat ccaatagtga ggttgattac cacagatcag aggataaacc
     5821 agactgctat ccagtgactt ctgaataact cacaaaaata aacatagatg attagaaata
     5881 gccatatcac aacagcgata atcgctataa ataacagtat cgtcacaaat aaatcagcaa
     5941 aggttttaat ggggagggag tgcaataatg aatacaccag taaagcaaaa gccgcattac
     6001 ctgataatga ctctcttatg atgtttctca ctatttccag tttttcttta aaagataact
     6061 ttttcatttg tacctctgat taatatcaca caaacgcgaa tattacagac atgaatactc
     6121 tcatgtaaat aataaccgga gtttaacatg ataagtaaat acagattctc tttaccccgg
     6181 cttgatatta cgggaatgtg ggtattttcc ctgggtgtct ggtttcatat cgtcgcccgt
     6241 cttgtttaca gcaaaccctg gatggctttt tttctggcgg agttgattgc ggccatcctt
     6301 gtgctgttcg gcgcatacca ggtgctggat gcatggattg cacgtgtcag cctggaagag
     6361 cgtgaggcac tggaagccag gcaacaggcc atgatggaag ggcagcagga gggcggacat
     6421 gtctctcact aaattactgc cggtcatttt gttgatgatg gccacaggtg tgcaggcctc
     6481 cacccgggat gaaattgaac gactctggaa tccgcagggt atggccgtgc agcctgcaca
     6541 accggcagca gacacatcag ccagaacaga aaagccggct ccccgctggt tccgcctcag
     6601 caacggcagg caggttaacc tggccgactg gaaggtggtg ctgtttatgc aggggcattg
     6661 cccttactgt caccagtttg acccggtact gaagcagctg gcacagcagt acggcttttc
     6721 cgtttttccc tacaccctgg atggtcaggg ggatactgcc tttccggaag cattaccggt
     6781 gccaccggac gtgatgcaga ccttcttccc gaatatcccg gtggccacac cgaccacctt
     6841 cctggtcaac gtcaacacac ttgaggcatt accgcttttg cagggggcaa cggatgccgc
     6901 cagttttatg gcgcggatgg ataccgttct gcagatgtac ggagaggaaa aaggtgcgaa
     6961 ataagcaggt ggtgttactc attgccggaa tatccggcat cgcgacggga ataatcgtca
     7021 gcctgaatat cccctttatc cgtcaggggc ttttttatcc cgccagccct gtggaaattg
     7081 tcgtttcgct gtgtcttacc ttttctgttt ctgttgtgtt ttttgtgggg gcaattgtgg
     7141 gatggatttc agtatctgaa atatattaca gctgcatggc ctgtctgaat gaatcatcag
     7201 aaatatcaga ggaaacttat gatgacagaa aacaaaaaga atcaggaatt caaaatcaga
     7261 aaaattaaac ggaatattga gcgcagttgt gataacgcca taaaatactt cgagcttttt
     7321 gtggttttct ttgttgccgg attgctttta tggcacggta tgcatgcttt tttcagcgcc
     7381 ggtattgatt cgtggaaagc tgacccggaa ttaaataatt tgatttatat gtggaacatc
     7441 ctgatgtatg ccattccgta tacgttatat gcgcttgcag ccgggtttct tgttacatct
     7501 tttctgtccc caatgtacga attaatattc ggaaatatca tgatattcct gctgaaacgc
     7561 cgtatgcgcc gggagaacaa attaagggag ggtggcaata atgcttcgca ttaaacctct
     7621 tctggttctt tgtgccgctt tactgacggt cacacccgca gcgtcagcgg atgtgaacag
     7681 cgacatgaat cagttcttta ataagctggg atttgcttcc aataccacac aaccgggcgt
     7741 ctggcagggg caggccgccg ggtatgccta tggtggctcc ctgtatgccc gcacccaggt
     7801 caaaaatatc cagctgattt ccatgacgct gccggatatc aatgccggat gtggcggcat
     7861 cgacgcctat ctcggctcgt tcagttttat taatggcgat cagttgcagc gttttgttaa
     7921 gcagattatg agcaacgctg ccggttactt ttttgacctt gccctgcagg caacggtgcc
     7981 ggaaatcaaa accgcaaaag acttcctgca gaaaatggca agcgacatta acagtatgaa
     8041 cctcagctcc tgtcaggtgg cgcaggggat tgtcggagga ttatttcctc agactcaggt
     8101 gtcacagcag aaggtctgtc aggacattgc cggtgagagc aatatttttg ctgactgggc
     8161 cgcgtcccgt cagggctgca cggtcggagg acagtcatcg tctgttcgtg acaaggccag
     8221 tgacaaagat aaggaacggg tgctgaagaa catcaatatc atctgggact cgctgtccaa
     8281 aaacaggatg cttgatggta acaaagaact gaaagaattt attatgacgc tcaccggtac
     8341 gctgattttt ggggaagaca gcgagattac ccccctgccg gcgcgtacca ctgaccgcga
     8401 tatcctccgg gcgattatgg aggggggaac ggcaaaagtt tatcactgca acgattcgga
     8461 caagtgtctg aaggtggtgg cggatgcaaa tgtgaccatc gcccgggata aagccctgaa
     8521 atcacagatt acaaaactgc tcaccagtat tcagaataaa gcggtgagtg atactccact
     8581 ggatgacagg gaaaaaggct tcatttccag caccaccgtc cccgtcttta aatatcttat
     8641 tgacccgcag atgctcggtg tgtccagcag cgtggtttac cagctgacgg actatatcgg
     8701 ctacgacatc atgctccagt acattcagga actgcttcag caggcccggg ccatgattgc
     8761 caccgggaac tatccacaga ccgtgctgga cagtgtgctg gagaatctga atcaggcaca
     8821 gcaacagatt gcggttttcc aggggcaggt aaaggtccag caggatgcat tgctggttgt
     8881 tgaccgtcag atgagctaca tgcgccagca gctgtccgcc cgcatgctca gccgttacca
     8941 gaacaactat cacttcggag ggagcacgct gtgaatgaag tctatgtgat tgccggtggt
     9001 gagtggttac gcaataacct gaacgccatt gccgccttta tggggacccg gacgtgggat
     9061 tccatcgaaa aaattgcgct cacattgtct gttctcgcgg tggccgtgat gtgggtacag
     9121 cggcacaacg tgatggattt gctgggctgg gtggccgtgt ttgtgcttat cagcctgctg
     9181 gttaatgtcc gtacatcggt gcagattatt gataacagtg acctggtcca ggttcaccgg
     9241 gtggataatg tgccggtcgg actggcgatg ccactttcac tgacgacccg tatcgggcat
     9301 gcaatggtgg ccagttacga gatgatattc acccagcctg acagcgccac ctacagcaag
     9361 acaggcatgc tgttcggggc gaacctgatt gtgaaaagca ccgattttct gtcccggaat
     9421 ccggaaatca tcaatctgtt tcaggactac gtccagaact gcgtgctggg ggatatttac
     9481 ctgaaccaca aatacacgct ggaagacctg atggcctccg gtgaccccta cacgctgatt
     9541 ttttcccggc ccagcccgct acggggcgtt tatgacagca ataataattt cataacctgt
     9601 aaagatgcgt cggtcacgct gaaagacagg ctgaatctcg atacaaagac gggaggcaag
     9661 acctggcatt attatgtgca gcagatattt ggcggcagac cggacccgga cctgttattc
     9721 aggcaactgg tgagtgacag ttacagttat ttctacggct ccagccagtc tgccagtcat
     9781 attatgcgcc agaacgtcac catgaatgcc ctgaaagagg gtatcaccag taatgcagcc
     9841 cgtaacggtg ataccgccag cctggtcagt ctggccacga cttcgtcgat ggagaaacag
     9901 cgtctggcac atgtctccat tggtcatgtg ctgatgcgaa acctgccgat ggtgcagacc
     9961 attctggtgg gcattaccat tggtatcttc ccgctcctgg tactggcggc tgtcttcaac
    10021 atactgacgc tgtctgttct gagaggctat gtgtttgctc tgatgtggtt ccagacctgg
    10081 ccgttgctgt atgccattct gaacagtgcc atgacattct atgcaaagca gaatggtgca
    10141 ccggtcgtgc tctctgaact ctctcagata cagctgaaat actccgacct ggcgtccact
    10201 gccggatata tatctgtcat gatacccccg ttgtcatgga tgatggtaaa aggccttggc
    10261 gcaggttttt ccagcgtgta cagccatttc gcttcctctg ctatcagccc gactgccagt
    10321 gcggcaggta gtgtggttga cggtaattac tcctacggca acatgcagac ggaaaacgtg
    10381 aacggcttca gttggagcac caacagcacc acgtcgtttg gtcagatgat gtaccagacc
    10441 ggcagtggcg caactgccac acagacccgt gacggtaata tggtgatgga tgcaagcgga
    10501 gcgatgtccc gtttaccggt tggtatcaat gcaacgcgtc agattgcagc ggcacaacag
    10561 gaaatggccc gggaggcgtc gaacagagca gaaagtgccc tgcatggttt cagcagcagt
    10621 attgccagtg cctggaacac gctcagtcag tttggttcta accgggggag cagtgattct
    10681 gtcaccggtg gcgctgacag caccatgagt gcacaggact ccatgatggc cagccgtatg
    10741 cgcagtgcag tggaaagcta tgcgaaggcg cataatatca gtaatgagca ggcgactcag
    10801 gaactggcat caacaggttc taagttctcg tatggcggat atgcagatgc tcatgctgaa
    10861 tgggggatta aacctggaat tcttggtgtt ggtggtgggg ttggtgtcag aggcgggttt
    10921 aagggaagtc ttgatcgaac ggatgatgat tcacattctg caagtagtgg cagcagagct
    10981 acccatgatg ctcgccatga tattgacgct aaagccagta aggactttaa agaagccagc
    11041 gattacttta ccagtcgcaa ggtcagtgag tccggcagcc atactgacaa taatgctgac
    11101 tcccgggtgg accagttgtc tgcagccctg aactcagcga aacagagtta cgaccagtac
    11161 acgacgaata tgacccgcag ccatgaatat gcggaaatgg cctcccgtac tgaaagcatg
    11221 agcgggcaga tgagtgaaga cctgtcgcaa cagtttgcac agtatgtgat gaaacgcgca
    11281 ccgcaggatg cagaagctat tctgacaaat accagttcgc cggagattgc agaacgtcgt
    11341 cgggcgatgg cctggtcttt tgtgcaggaa caggtacagc ctggtgttga taatgcctgg
    11401 cgggaatccc gtggcgatat tggtaaagga atggagagcg taccttcggg tggtggcagt
    11461 caggatatta ttgctgacca tcagggacat caggccatta ttgagcaaag aacgcaggac
    11521 agtaatatcc gtaatgatgt aaaacatcag gttgataata tggtcacaga atatcgtggc
    11581 aatatcggag atactcagaa cagtatccgt ggcgaggaaa atattgtaag agggcagtat
    11641 tctgaactgc aaaatcatca taaaacagag gcattatctc agaataataa atataatgaa
    11701 gagcgagaaa gtcaaaagat aatacctgag ccatcagaag aggctgtgaa aaaaatggtt
    11761 gatgataaaa aggatagact gaaggggcca ttatgatcac tcactatgat ataaaaacgg
    11821 agacacagaa actaaaggat gtactctctg tagagggagt taatattccg ccattattac
    11881 aagtcataaa accaggggtg tatgtgtttt tatgggtttt attatggccg acatttcttc
    11941 gtttgctggc agataaagtt gatatccgag atgctggatt tgatatatgt tttagtggcg
    12001 taatgggttt tatccttttt gttgccatta ctaatggaat gatgttgtat cttgccatcc
    12061 ctaaaaaatt cagagatgag tcgaaagtta tatcttttat gtatgataag aacaaaactt
    12121 atatcttgtc gtttgtgatt gttttttcaa tagtttcctt tgctcatacg ttcttatttg
    12181 ggtttttatc aataactctg tttgtaatta tttcctttat ttatactata gatataaatc
    12241 gttataatct ctctgcaata gtctcagtaa taggtctgtt caaaaaagag tctgttagtt
    12301 aaaaggatat tctggatgaa aacaaaaaaa atgatgatgg ttgcactggt cagttccact
    12361 ctggcccttt cagggtgtgg tgcgatgagc acagcaatca agaagcgtaa ccttgaggtg
    12421 aagactcaga tgagtgagac catctggctt aaacccgcca gcgaacgcac ggtatttctg
    12481 cagatcaaaa acacgtctga taaagacatg agtgggctgc agggcaaaat tgctgatgct
    12541 gtgaaagcaa aaggatatca ggtggtgact tctccggatg cagcctacta ctggattcag
    12601 gcgaatgtgc tgaaggccga taagatggat ctgcgggagt ctcagggatg gctgaaccgt
    12661 ggttatgaag gcgcagctgt tggtgcagcg ttaggtgccg gtattaccgg ctataactca
    12721 aattctgccg gtgccacact cggtgtaggc cttgctgctg gtctggtggg tatggctgca
    12781 gatgcgatgg tggaagatgt gaactatacc atgatcacgg atgtgcagat tgcagagcgt
    12841 actaaggcaa cggtgacaac ggataatgtt gccgccctgc gtcagggcac atccggtgcg
    12901 aaaattcaga ccagtactga aacaggtaac cagcataaat accagacccg tgtggtctca
    12961 aatgcgaaca aggttaacct gaaatttgaa gaggcgaagc ctgttctcga agaccagctg
    13021 gccaaatcaa tcgcaaatat tctctgaacc tcgtcaggag gctgatctga ccggcctcct
    13081 tgctgacgcc tcgcgttgct cgttgtccaa ccccgggatg taacaaaata aattttgtca
    13141 cctcccggcg tttccgaaat aaatatgaaa acactgagcg gttgtgcaaa ttctttgtgc
    13201 gaccgtgaag tgttgccggc gaagccggga tcttccggag aaagtcatta tgacatcaga
    13261 caatatcgct gaatatattg gtgagaataa tcaatttact gagaagcctg caactgaata
    13321 ccccccggtg atacaggata atacagtgac cactaaagac tatctgtaca gcaataatat
    13381 tgatactgac cttttagtgg ttatcggagg gcttattgtt atattgtttc tcatgatgct
    13441 tatcatgtac tttattaatt ctctcaggca taaggaatta actgaaatac tgacaaaaat
    13501 atcttttcct gaaaaatccg ggagcaattt gcttaaaccg gaggcggtca gctgtaattt
    13561 gttgtccctg tgtgtgctgg caccggtatt ctgtacaccc agaaagctgg ctgaagtttc
    13621 cagggagatc atcgtggcga gtgcgcagga aattgcatgg acgccgggga gcagtcgtat
    13681 tgatggccac caggcggaga atatcacctg ggcgatttca tcgcagtctc ctgccgataa
    13741 gtatgatctc atcaataaaa cgtcctgtgt ttctcctgac agtggccgtt tttatgaacc
    13801 ggacacagtt ctggatcacc gtgaacgggt acgccttttt attcagatcc agtttccgca
    13861 tgggagtgac tggtctgaag tattgcgcct gactgacatt gttacagaga aggtctcttc
    13921 cggcaggact gcaggcgggc atcaccaccg gcatacaggg tacacctggt ctgtttctgt
    13981 gaattaaccc cacatcatcc ttttctgccc tgttccagat tttcccggag tttcatcatg
    14041 agttttaacg caaaggatat gacccagggc ggtcagattg cgtccatgcg tatccgcatg
    14101 ttcagccaga tcgccaatat catgctttac tgcctgttta tttttttctg gatcctcatt
    14161 ggtctggtct tatgggtaaa aataagctgg cagacgttta tcaacggctg tatttactgg
    14221 tggtgtacgt cactggaagg catgcgggat ttaataaaat cccagccggt ctatgagatc
    14281 cagtattacg gcaaaacctt ccggatgaac gctgcccagg tactgcatga taaatatatg
    14341 atctggtgcg gagagcagct atggtctgca ttcgttctgg cgtctgtcgt ggccctggtt
    14401 atttgcctga tcaccttctt tgttgtctcc tggattctgg ggcatcaggg gaaacagcag
    14461 agtgaaaatg aggtgaccgg cgggcgtcag ctgacggata acccgaaaga agtggcccgg
    14521 atgctgaaaa aagacggcaa ggactccgat atccggattg gcgacctgcc gattatccgg
    14581 gattccgaaa tccagaactt ctgtctgcac ggcacggtcg gggccggtaa gtcggaggtt
    14641 atccgtcgtc tggccaacta cgcccgtcag cgcggtgata tggtggtgat ttatgaccgt
    14701 tcaggggaat ttgttaaaag ttactatgat ccctccatcg ataaaatcct gaatccgctg
    14761 gatgcccgct gtgccgcatg ggatttgtgg aaggagtgcc tgacacagcc ggattttgat
    14821 aataccgcaa atactctgat ccctatgggt acaaaagagg acccgttctg gcagggttca
    14881 ggacgtacca tttttgcgga agcggcgtac ctgatgcgta atgaccccaa ccgcagctac
    14941 agcaaactgg tggacacact gctttccatc aaaattgaaa aactgcgtac cttcctgcgt
    15001 aattcaccgg cggccaacct ggtggaagag aaaattgaga aaacggcgat ttccatccgt
    15061 gctgtgctga ccaactacgt gaaagccatc cgttacctgc aggggattga gcataacggt
    15121 gagcccttca ccatccgtga ctggatgcgt ggtgtccggg aagatcagaa aaacggctgg
    15181 ctgtttattt cgtcgaacgc cgacacccat gcctccctga agcctgtgat ctccatgtgg
    15241 ctgtccattg ccattcgtgg tctgctggca atgggagaaa accgtaaccg tcgtgtgtgg
    15301 tttttctgtg atgagttacc cacgttacac aaactgccgg atctggtgga gatcctgccg
    15361 gaagcccgta agttcggtgg ctgttatgtg tttggtatcc agtcctatgc ccagctggaa
    15421 gatatctacg gtgagaaagc ggcggccacg ctgtttgacg tcatgaacac ccgtgctttt
    15481 ttccgttccc cgagccataa gattgcagag ttcgctgccg gtgagattgg tgagaaagag
    15541 cacctgaaag ccagcgagca gtattcctac ggtgctgacc cggtacgtga cggggtatcg
    15601 accggtaagg atatggagcg tcagacgctg gtcagttatt ccgacattca gtctctgccg
    15661 gatctgacct gctatgtcac tctgcccgga ccgtatccgg cagtaaaact ctctctgaaa
    15721 tatcaggcac gaccgaaggt cgctccggag tttattccgc gtgacatcaa cccggaaatg
    15781 gagaaccgtc tgagtgccgt acttgccgca agggaagcag aaggtcgtca gatggccagc
    15841 ctctttgaac cggaggttgc ctccggagaa gatgtgactc aggct
//
LOCUS       DAZPDU010000097        15548 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZPDU010000097 DAZPDU010000000
VERSION     DAZPDU010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15548
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.098"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(60..254)
                     /locus_tag="ACHURS_002741"
                     /pseudo
     CDS             complement(60..254)
                     /locus_tag="ACHURS_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(497..1546)
                     /gene="yahK"
                     /locus_tag="ACHURS_002742"
     CDS             complement(497..1546)
                     /gene="yahK"
                     /locus_tag="ACHURS_002742"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFR1238425.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMIGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAEKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIADSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            complement(1633..1707)
                     /locus_tag="ACHURS_002743"
                     /pseudo
     CDS             complement(1633..1707)
                     /locus_tag="ACHURS_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1923..3305)
                     /locus_tag="ACHURS_002744"
     CDS             complement(1923..3305)
                     /locus_tag="ACHURS_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFR1238426.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTYVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLKQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            complement(3315..4265)
                     /locus_tag="ACHURS_002745"
     CDS             complement(3315..4265)
                     /locus_tag="ACHURS_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFR1238427.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTDVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFSPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            complement(4408..5826)
                     /locus_tag="ACHURS_002746"
     CDS             complement(4408..5826)
                     /locus_tag="ACHURS_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFR1238428.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            complement(5826..7373)
                     /gene="fdrA"
                     /locus_tag="ACHURS_002747"
     CDS             complement(5826..7373)
                     /gene="fdrA"
                     /locus_tag="ACHURS_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFR1238429.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPTLEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGSKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            complement(7363..8226)
                     /locus_tag="ACHURS_002748"
     CDS             complement(7363..8226)
                     /locus_tag="ACHURS_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFR1238430.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWLQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQTVNDKQNI
                     AAAVASMIGLGIGLTPSADDYLTGLALILFIPGHPAEKYKEEFYRGLLRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYGVPGNSTQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            complement(8266..8871)
                     /locus_tag="ACHURS_002749"
     CDS             complement(8266..8871)
                     /locus_tag="ACHURS_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFR1238431.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            9129..9626
                     /locus_tag="ACHURS_002750"
     CDS             9129..9626
                     /locus_tag="ACHURS_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFR1238432.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALTAGWLASVSGLSAFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            9718..10650
                     /locus_tag="ACHURS_002751"
     CDS             9718..10650
                     /locus_tag="ACHURS_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR1238433.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFYAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMTQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(10692..11780)
                     /locus_tag="ACHURS_002752"
     CDS             complement(10692..11780)
                     /locus_tag="ACHURS_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFR1238434.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(11922..>15548)
                     /gene="ehaA"
                     /locus_tag="ACHURS_002753"
     CDS             complement(11922..>15548)
                     /gene="ehaA"
                     /locus_tag="ACHURS_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HFR1238435.1"
                     /translation="TGFLNVSNAGSELYADDLYESNSGMRHDRGYFNVSNGGKIHVKG
                     TSRLTYLQGNVSGEGSQVNSKTFFMGVYGSYGGNQYLSVNNGGEVNASEKISLGYYDQ
                     NSDTTLAVSEGGKISAPTISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKTSEK
                     KITLNHTDKNATISADIVSDSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGALGIT
                     QNIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVALTGNNYAFSGYIDVAS
                     GAVAVISEDKNIGRADLDVDGKLQINANKDWVFDNDLQGRGIVEINMGNHEFSFDEFA
                     YTDWFQGSLAFQNTTFNLEKNAEFLQRGGITAGQGSLVTVGKGAHSISTLGFSGGTVD
                     FGALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVRSVSRDIDSALSLTEVDDGNS
                     AIKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTSGVN
                     NDGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKGQTVS
                     LSNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNGSAD
                     SLLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSANVLV
                     SHAQGLGSANVENNGTLALNNSAEKRAAASVNYTLGGNLTNNGTLMTGMSGQQAGNVL
                     VVKGNYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLNGIE
                     LIHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANSGNWYLTSGSDSPELQPEPDPMPN
                     PEPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGNTYY
                     TDMVTGEQKQTTMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDVAQWSQNGSDSWH
                     VGVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQYSW
                     FDNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGSQGTRNEWYVQPQAQVTWMGV
                     KADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLHNSK
                     DFSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAMVGI
                     KWQF"
BASE COUNT         3556 a         4233 c         3715 g         4044 t
ORIGIN      
        1 ttaagactat cacttattta agtgatactg gttgtctgga gattcagggg gccagtctag
       61 tcgtcatcga ctaaatcatc acgacaatca taaggataat cataatattg tttaatcaaa
      121 accttgaata tatcgacaca ggaaaccagg gagcttttga tatcctctgg taactgattg
      181 tttttaaccg tattaagtat gctttgttgc gtatatggca tcaaattatt gtgcggagct
      241 tttaatgata tcatcgcgat ctctcttaaa taaaaataaa cgtcgcagat aataaagcgc
      301 tgattaaacc aggcactatc agaaatcgct cataactaac gtgagaagta ttgtgtactg
      361 gagggagtta atttaacaat ttgaatttac gttataggtt ttactgctac tttgatatta
      421 tttcgctctc cttaactaaa aagttaagtg gagagcgaag ttaatatgtt aaaccacagc
      481 gtatttatta attttttcaa tctgttagtg tgcgattatc gataacaaaa cgatatttca
      541 catcaccacg cagcattcgc tcataggctt cattaatttg atcggcccga atcatctcta
      601 tatcagccac gatgccatgt tcggcgcaaa aatcgagcat ctcctgagtt tctggaatgc
      661 cgccaatcat agaatcggct atcgcacggc gtttcatgat caggttgaaa acttccggcg
      721 atttatgcgg tgtcgcaggc gcaccaacca gcgtcatggt gccatcacgc ttcagcaagg
      781 tggtaaaatc gtcgagatta tgtggcgcag ctactgtatt caaaatgaaa tcgaaactct
      841 tcagatgagc cgccatctca tcggcattgc gtgagttaac aacttcatcg gcccccaggg
      901 ctttttccgc ttcgcgtttt gcctcagaag tggtaaatgc caccacatgt gcccccatcg
      961 cgtgggccag cttaatcccc atatgtccca gaccgccgat gccgaccacg cccacttttt
     1021 tacccggccc ggcctgccag tgacgtagcg gcgagtacgt ggtgatccct gcacacaaca
     1081 aaggagccac cgccgccagc tgctcttgcg ggtgacgaat acgcagaaca tatcgctcat
     1141 gaacgacgat ctgttgtgag tagccgccca gagtatggcc cggttcgtcc ggcgtcggcg
     1201 agttataggt gccgatcatg tgatcacagt agttttccag cccgtcctca cactcttcgc
     1261 aatgtttaca actgtcgaca atgcagccga caccgaccag atcgcccggc gcatgttttt
     1321 ctacctgatc accaacggct accacacgcc ccacaatttc atgacccggt acgcaggggt
     1381 aaaccgtccc cgcccactcg gaacggacct ggtggagatc ggaatggcag acgccacagt
     1441 aagcgatttc gattttgaca tcattcggtc ccggttcacg ccgtgtgata tccatcggtt
     1501 caagtggttg tttagcggaa tatgcaccaa cagctttgat cttcattgtg tttactcctg
     1561 attagctatg tgtatttggc gacgcctgat atgtcatcgc ggcaaaacgc gtccattgaa
     1621 tatagccaat atttaaacca cccgatccag ccccaacatg ccgcgaacct gaatgaaaca
     1681 gagcagcaac gttccccata aggcgagcgc ctataagtgt gatatttgct atccgtaaaa
     1741 gactaaaatc ggcaaaactg atgatctttt cacgtagaca aatagttatc tacacaacgt
     1801 caataagggc atattcctca aagataatgt ccatttctgc cgacactatc atattgaggt
     1861 cgccccactc cctggggctt aaatctctta caattcaccg ttgcaggcgt ttcaaatgcg
     1921 catcaacctg ccacactccc ccacaccagt tgccctttat ggaacgttgc ggttctcggc
     1981 gagatacgcg ccaccgcctc ggcggaacag gaggcgtcca ccagcacaaa gctggcatca
     2041 tcctgcgctt ttggccatac acgctcgcct ttttcattca gcggcaatac atcgccagtg
     2101 gctagaaaca gcgaacggga gaggttctgt tcgttaggac gaatatagag ctgcgcgtag
     2161 agattggctt tttccagcat gtcgcccaga ccataaggcg accagtggtc gataacgctg
     2221 tcagtgccag tcatcacttt tacgcctttg tcgtgcaact gtttgagcgg catatgcagc
     2281 gtgccaatcg gcaccgtcga ggcgatagaa atttgctgcg ccgccatccg attcgccagc
     2341 gcatcgacct gttgttcgtt gagcgtagcc agcgcaaagg cgtggctgat ggtcagctta
     2401 cctttcagtt gcggcgtttt ctctaccgtt tcaaccatat aattgatggc tgccacgccc
     2461 gacggagtgg tttcgtgcag gtgaatatcg acacctttgt cgtagtccag cgcaatctga
     2521 aacatggtgt cgagggattt ttccatcgcg ccatcgacac tggtcgggtc cagcccgccg
     2581 acgtaatgcg cacccgcctg catcgcttcg cgcattaagg gttccgattt cgacagcagc
     2641 aaaccgtgct gcgggaaggc gacgatttca cactcgaagc ccgcctgacg ccgcgccagc
     2701 accgcctgca aattttgcag attttttagg ccggaaaccg gttcgatatt gcagtggctg
     2761 cgggcaatgg tggtgccttt cgactgcaat agatcgatca gtttttccgc ccgctcctgc
     2821 gtatacggtt gtaactccgg cagcattttc tgcttgagtt tgatcatgtc ctggatagtg
     2881 gtgcctgccg gacgattgag cgagcgccac ggcccgccgt agaaggtttt gtcgagatga
     2941 atatgcatgt cgcgagtggt gggtagcatc aacttaccgc cagcgtcata gtgcggcagc
     3001 gtggcgtccg gatgctgctt gttctcgcgc agggcgacaa ttttgccgtc ctgaatctcc
     3061 acggtctggc gcgccgtgcg ggtctgcacc gccacaccgt tttcgtagtc aaaaccggtt
     3121 tccagcagca cgttatcgag atagtaatgc gggtcagtga ttttcatggt gttgttcgct
     3181 tcgcagttta gggtgccagc taccgccgca tgggcgagcg gcacataggt accaaacagc
     3241 gcggcggcgg tgaccatctt accgctctgg ctcagaaatt cacggcggct attgctttct
     3301 ttcatcctac gtccttacgt tttaataata tgggtgcccg tttcaccgcg cagcgccgca
     3361 ggcaggcatt ccggcgtggt gataatcact tctttgccgc cctgctccag gaacgtcagg
     3421 ctggcgatga tttttggcaa catgctgccg ggagaaaaat gcccttcctg catatagcga
     3481 gtcatggtgg caatatccac ccgatccagc gcctgctgct gcggtttacc aaagtggata
     3541 cagacttttt cgacgtcggt ggtgatcacc agaatgtcgg cgtgaatttc acgggccagc
     3601 agcgcggtag agagatcttt gtcaataacc gcgtccacgc tttggtaatc ccccgcttca
     3661 gtacgcacta ccggaattcc accgccgccc gcgccaatta cgacaaaacc ttgttggatc
     3721 agcgccttaa tggcgggtgc ttcgacaata cgtttcggtt ccggcgaggc gaccacgcga
     3781 cgatagcccc gtccggcatc ttcaacaaaa caccagtcag ggtttgcctt ttgtagtttg
     3841 tcacgctggc tttcactgaa gaatgcacca atgggcttgg tgggatgggc aaaacctgga
     3901 tcgtttttat ccacttccac ctgagttacc acggtgacgg cttttttctc gccgtgacgc
     3961 gccagtcggt tattcagcgc ctgttggatc agatagccga tgccgccctg cgtatctgcc
     4021 acacagttcg ccagcggcgt taagggcagc ccttcgcgct cgtgggcaat ctccgcacgg
     4081 cgtaaatcca gcccgacctg cggcccgtta ccgtgggtca gcacaatgtc gtaatcggaa
     4141 gccagcattt ccagcaccgt atcggccacg gctttcaccg cctccgcctg atgctcaatc
     4201 gactggctgg cgttatcttt gataatgctg ttgccaccaa tggcaacgac cacaagctct
     4261 ttcatgttgt tatgtccgct acagagggtg atgaggttat gaccaacgcc agactgcctg
     4321 atgcgctacg cttatcaggc ctacaattct cgggccatcc gtaggccgga taaggcgttc
     4381 acgccgcatc cggcataaac atgtaagtca gacgccgtaa cgttcgcacc agccgagaat
     4441 ggctttctcg aagcaagcga atggtggatg cacaatgccc gcgccaatca tgccgacgcc
     4501 cgcatcttta tgggcgatgg cggtgttgat caccggcaga atgccgctgc tacccacgcg
     4561 ggtgatgtcg attgccgacg gcacgcccat aaaaccgagc agcggaatgg tgacgttggg
     4621 gttttcaccg agagtgattt cacgcatctg acgggagaaa tcgatagcct cttccaccgt
     4681 gccgcccacc agcgcgacga ttgcaggcgc ggtcgccata gcaaatccac caataccgta
     4741 ggtttcggtg atggcgctgt cgccgatatc cagccccgaa tcttccggct tataaccggc
     4801 aaacatcggg ccgatcacct gctgcgccgg gccggtaaac cattgccccg gcaggccgct
     4861 gacccgcagg ccgaactcga cgccgttacg cgccatggtg gtgaccacgg tgctgtattc
     4921 gatgccgtgc gccgcatcca tcgccgcttt acacatcgcc atccacgtcg ggccggagaa
     4981 gtagtcgctg ctggcgacaa actcgaacac ttcgcgctgt tgctcgacgg aatagcctgc
     5041 ctgaatgatc cccggcgtca gtgcctgaat cagcagcgtc gtcccggcgt tattgcggtt
     5101 atggcactca tcgcccatat gcagcgcctg cgccagcatt aagcgcagat cgatctcacc
     5161 gataattttc atcgcgtcgc gcagcattgg cccctgcaca tcgcgcatcc agttgaggcg
     5221 gtcgatcacg ctctgatcgt tggcacccat acgcaaaatc ttcgccatct gctcgctcat
     5281 attggtgtaa gcgatgttgc cgtaggtttt gtttttcacg atgtgcataa acatcgaggc
     5341 cgaggtaacg cccgccatcg atcccacaca gtcgtgttcg tggcacggcg agaaggtgat
     5401 ctccccggag gccgccagtt cagccgcctc gtcgagatct ttcgccagtc cttcgaacac
     5461 cagcgctccg gtgaccgcgc ctttcatcgc gccgcacatt ttttcccagg tgatcggcgg
     5521 cccggcgtga agaatggttt tcggcgtcat gcccggcacc acgttaatcg cctgatcaaa
     5581 accaatcagc accggatgcg actggataat gcgctccagc gcctgctggt tagcggcggc
     5641 gattttgtct gccagcggcg aatcggcaat gttatccagc gcctgcacca cctgcatatt
     5701 gccctgcccc ggcggcgtcc agtcgagttg agtcacttct acatgctgct ttttcaggtc
     5761 atcgctaaac atggcgatgc cgacgttaat aacgttcaat ggttggctaa acagtgactg
     5821 gctcatcagg cttcctcccc tttgcagata aattcacgcg ccagcaatcc ggtattggtg
     5881 ctgctgctcg ccagaatcac tccggcatca agcagcatct ggctttgttg ttctaacgat
     5941 ggcgtatcaa gatcggtacc cagcacatag gcgagaatga tcaactcgcg tccttcggca
     6001 gcggcgatcg ctttcgcttc tttgatcgcc tcgatggtgg agccgaccgg atcttcatgc
     6061 gatccaaatc cgagcacaaa atccatcacg atcaccgcca cttctggatc gcgcgcctct
     6121 tcgatcaagc gactgatgcg gttggtgggg tcaatcattg gatgcggctt gccgttcgtg
     6181 aagtcgtcat cgccaaagtc gaggaaggtg tgtttgatgc tgcggttgat atcttgcagg
     6241 cggaattccg gatcgggctg aatgttgctg tagacatcgc catgtttttc catcaccgcg
     6301 aacatggttt cgtcgcacag cgtgccgccg cagaacaggc cacgaatgta tttctgctgc
     6361 ggttgcagac gcgcacgcac atcagcaatc aacggctggt taagcgtatg caggtcgaga
     6421 ttttcctgtt tcacgccgga gagcatcacc gcttttagcg ccgcctcttt gctgccgcgg
     6481 gcaaactgta gcccctgctc atccaccggc gtttcaacgc gaccgaggaa gcagaccacc
     6541 accggcttgc ggcaggcacg tgcgcgttcc agcactttgc gggccaccgc aggcgcggga
     6601 ggttttgaaa tcagtgcgat aatttcagtt tgcggatcgt tttccagcat cccgatggcg
     6661 tcgagcatca tcaggccgcc gattttctcg ctcaggtcgc gcccgccggt gccaatcagt
     6721 tgtgaaacac cgccaccaaa ttcatgaatg cggacgctca gctcctggct gccggtgccg
     6781 gatgcgccaa caataccgat attgccgcga cgcacggcgt taccaaaaca gagcgccgcg
     6841 ccgttaataa tcgccgtgcc gcagtccggc cccatcatca gcagcccttt ttcgtgggcc
     6901 aactgcttga gcgccagttc atcttcaact gagacgttat cggaaaacag catcacgttg
     6961 agatcgtttt gcagcgcctg acgcgcttcg cgagcggcaa acagaccgtt gaccgaaatc
     7021 accgccaggt tactttccgg gatatgcttt ttggcgctgg caatagtggc gtaacgcgcc
     7081 tcgtgcgagc cgctttgcgc tttggtgttg aacagttctt caatctccac cagcaactgc
     7141 tcgttgtccg cacccgattt accattgatg acaatcatca ggtcgccgtt tttcgcctgt
     7201 tccagcgtcg gcgtcagcag tcctaaattt ttcagcacac ctttgttcat ttcggtcgcc
     7261 atcgccacaa atgcctgctc gacgccgtcg agtttatttg cacgcgtgga gatagacatc
     7321 agcgagacag aatcaaaata ggtattcggt ttaatgacta ttttaactga cataattccc
     7381 tccgtaggtt tggctcagcg cacaaccatc ggccatgcca tacagcatgt cgcagccgga
     7441 actggagcca atatgtttaa ttttttctat tgcctgagtt gaattcccag ggacgccata
     7501 aataatgttg tgaataaagc gatgaatatt ttcccggcag cgttgttgta atgcggcttc
     7561 cagcgttatg gcacttaata acgtggtgtt atttctgccg cgtagcagac cgcgataaaa
     7621 ttcctctttg tatttttctg ccggatgccc gggaataaat aaaataagcg ccagacctgt
     7681 taaataatca tcggctgatg gcgtaaggcc aatccctaac ccaatcatac tggcgaccgc
     7741 tgcggcgata ttttgcttat cgtttactgt ctgaataact gcccgtcgtt taatatgtaa
     7801 ttctttattt aacgcctgat aaaacggatt atcgccgcga taattaaaca gggtttcatt
     7861 ttcccccagg tgctgatgaa taatgtcgtg ccactgtagc caggcgataa ggtgaaaatt
     7921 cacagcggtc atttgcaagg ttggggacag ccaacgccga caacgactca tctctatatg
     7981 aagatgttga ccaaccgtaa taccttgatc gtcaaactga accttatcac catgccggaa
     8041 cagatcatca aagtgagtga gtgccaaccg acaactgttt ggcgcattgt cgtactcttc
     8101 acaaagcagc gtcagcaact gctggcgagc cgggagtgcg atatttaccg ctctggcaaa
     8161 gacctgttca acctgtcctt gcccccgctg cgccagaaaa tccgcatccg cagttagcgc
     8221 ccacacacac cgtttttctg gtgtgttgat agccagcctc ccggtttatg cacctgcggc
     8281 aatcagtaac tgcgcaatct cttcaaagcc ccgttcccgc gccagttcca gtggcgtttt
     8341 gccatattta tcggtcagat gcgggctggc accgtgttcc agcaataact gcacaatcgc
     8401 ctgctgttta ataccaccat cattaagcac aatcgcttcc agtagcggcg tccagcctac
     8461 atggttggtc tggttaacgt taatctccgt atgcgccaaa agctctttta caatacttaa
     8521 atggcctttt tcacaggcag gcgtcaggcc gacaccgcca aaacgggtta cgcaattaag
     8581 atcgggttta gccggtaaaa taattcgtag tagcgttaaa tcatcgttca gacagctaat
     8641 taaaaaagga tttaaacagg tatgatcttg cttattaata tccgctccgg catcaattaa
     8701 tgcctgaacg caagcatatt gctgatataa acttgccagc gtaattgccg ttttcccctg
     8761 acgatcgcag gtattaatat cgacacccag cgcaaggcag gtttttactt tatcgatatc
     8821 accctgttgt gcagccaata aatagtcggc aggtagattt tttatactca ttcacattcc
     8881 cctggttttt gtggctgttt tcgatgaatt gagaatagca acgataatct gataaaagaa
     8941 cccgcttaaa tttaattcgc cgtatctcgc ctttttgttg aataatattc aacaatcttt
     9001 tcgcagtgtg cggtagtgca atatttgttt ctttgacccg ataccctttc actcaatcag
     9061 gttttatttc tctatagtct gcacatgtaa gagtagcttt cgtccatgtc taaccagagg
     9121 ggaacaagat gaacggactc acggcaacgg gtgtcaccgt cggtatttgt gcagggcttt
     9181 ggcagttagt gtcttcacat gtcgggctct ctcaaggatg ggaattatta ggaaccattg
     9241 gcttcgtcgc cttttgcagt ttttatgccg caggcggcgg taaatcgggg tttatcagaa
     9301 gtctggcggt taattattcc gggatggtgt gggcgttttt cgccgcgctg actgctggtt
     9361 ggctggcctc tgtaagtgga ctgtccgcgt tctgggcgag cgtaattact accgtaccct
     9421 tctcggcggt agtggtctgg caagggcgtt tctggttact gtcctttatt cccggtggct
     9481 ttctcggcat gacgctgttt tttgccagcg ggatgaactg gacggtgacg ttactgggtt
     9541 ttctggcggg taactgcgtg gggataattt cagaatatgg tggacaaaaa ttaagcgaag
     9601 cgacgactaa acgcgacggg tattaaaagt tattcaaaaa tgattatcta tctttaaccg
     9661 atacctctac agtcatagct ggagaaattc atagtagata atggcgagaa tggtttaatg
     9721 aactcaattt ttaccgaaga gaatctgttg gcatttacta ccgctgcgcg ttttggcagc
     9781 ttcagtaaag ccgcagaaga gttgggttta accacttccg ccattagcta caccatcaag
     9841 cgtatggaga cggggctgga tgtggtgctg ttcactcgca gtacccgcag cattgagtta
     9901 acggagtccg ggcgttattt tttccgcaaa gctaccgacc tgctgaatga tttttatgcc
     9961 atcaaacgca gtatcgatac tatttcgcag ggcatagaag cgcgtgtgcg catttgtatt
    10021 aatcagctgc tttatacgcc caaacacacc gcacgcttgt tacaggtgct gaaaaagcag
    10081 tttcctacct gccagattac cgtcaccacc gaagtgtata acggcgtctg ggatgcgatt
    10141 attaataatc aggccaatat cgccatcggc gcacccgata cactactgga cggcggcggt
    10201 attgattaca ctgaaattgg cgcgattcgc tgggcttttg ctatcgcccc ggatcacccg
    10261 ttagcctttg tgccggaacc catcgccgaa agccaactgc gtctctaccc taatattatg
    10321 gtggaggaca ccgcgcatac gattaataaa aaagtgggct ggttgctgca cgggcaggag
    10381 tcaattctgg tgccagattt caacaccaaa tgtcagtgcc agattttagg tgaaggaatt
    10441 ggttttttgc cggattacat ggtccgtgag gcgatgacgc aatccctgct ggttacccga
    10501 caaatccata atccgcgcca ggattcgcgc atgttactgg cgactcagca ttctgctacg
    10561 ggtcaggtga cgcagtggat taaaaaacaa tttgcgccaa atggcatatt aacggggatc
    10621 tatcaggatt tactgcatcg ggagaattaa tcgctgccaa cctcccgatg cgctgtgaat
    10681 agcggtttac atcaaccacc tgctttcatt acccattcag agataaattt attacccggt
    10741 actggcggag agtacaaata gccctgcaaa aagtgaacgc ctttaccgat cattaaatcc
    10801 gcctgctcct gggtttctac cccttccgcc acgatactca gaccaggctt acgcgctagt
    10861 tcgacaatat tgtccacaat atgaccggag atttcgtcga cactcgccat ttgcacaaat
    10921 gacttatcga tcttaataaa atcgaccggg aacgcctgca agtaacgata ggtcgcataa
    10981 cccgtaccaa agtcatccag cgcaaaggta atgttgtgct gatgaaggct gtcaaatatc
    11041 gctctggctt ctggcgttac cggaataggg ttacgttccg ttagctcgag aaccagcttg
    11101 attttatcgt tacctaattt cttaaccagg ttcagacact ctttttcaaa tcccgctgcc
    11161 aaaaaacaac ccgccgagac gttgatgcca atatggaaat tgtccggcag caaatgtttt
    11221 accggcatca gaatatccgc agtctgtttc atcagttggc gggtcattat gacaataaga
    11281 ccggatgact ccgccagagg aataaactga tccggtggga taattcccgt ttgtggatgt
    11341 tcccagcgga caagtacctc acagcccgtc agtaccccag tctgcgcgca gaaaaccggt
    11401 tggatccacg gtttgaattc gtggttttcc aacgccagac tgatggcttc aggcgtgacg
    11461 atatggtgac aacggttatc agggatataa aaattatttt tatttcccgt gccactgatg
    11521 acttgcggat gatattgaaa aaagatatcc cgccagaagg taatatcact ctggatgcct
    11581 agtttttcat acagctgctt cttttgatgt gaaattgtct ttgcactgcg atttctgtac
    11641 tgtgagattt ctgtaaccga cattccctcc attagcagct tgagtaaaag ccgttctttt
    11701 tcggacacga taccaggcaa cgtcaaattc accgttgccc cgaactcttc caaaaaaaca
    11761 ctcaaatcat gcgaagccat tgaacacacc ttgattaaat cttgtgcgcc agctaaatct
    11821 gcgagtcttc actctgactg ataaagctgg ctgcaagaaa atgacacaga gagccgaatt
    11881 atctcccgtt cggctctctc tccttttact cattattcag atcagaattg ccacttaatg
    11941 ccaaccattg cagaggtgtc attatatccc ctatcggcaa cctgaacgcc cacattcccc
    12001 cagacattca ggttggcatt tagctgtcct tccaccccgg tttttatctc agcaatattt
    12061 cgggctccat cctgagtgac ggacacgcca tccatactgg tgctgaaatc cttactgtta
    12121 tgcagccagt tcacttctac aaacggctgg aactcgcggg atttaccgtc atccatttta
    12181 tggtggctct tcagccaggt ttttacgcca agtcgggttt gaacattgcc atcaccgttg
    12241 ctatgaacga gggttccgtt gctttcgcgg tgcttatcgg ctttgactcc catccaggta
    12301 acctgtgctt gcggctgaac ataccattca ttacgcgttc cctggctgcc attaaattca
    12361 gctaatttgt gtttgtatcc agcttccagt gaagcggtaa atccttttga tttataggat
    12421 tcactttgta agtcatcccc tttcactgtg ttatcaaacc agctgtactg cgcccaactg
    12481 tcgagatacg cgccattacg cgactcgtca tcggcatacc aggtggcata gaggccggtg
    12541 ctatatccgt tcacactcgc ttttgcacga taaccggttc gcgaggaaat ggttttgctg
    12601 tcgctgttgc catatcccgc catgacccca acatgccagc tgtcgctgcc gttttggctc
    12661 cactgcgcga catcgcctcc cagttgcaga acatagcgat tgctttgggt tttcagctgg
    12721 ccgctgccat cacgccattt attatgacca ccttcatggc gcatccacat agtggtttgt
    12781 ttctgctcac ccgtcaccat gtcggtatag tacgtattac ccagacgctc atgcagacgc
    12841 gtggtgaaca ttgtattcgc tgctgcaagg ttcgcaatgt agctacccgc ctccggtcgc
    12901 aggtcattat ccacattcag atccggaccc ggcgtaggtg tcgggttagg gttcggctct
    12961 ggattcgggt ttggctccgg attcggcatc gggtctggct ccggctgcag ttcaggagaa
    13021 tcactgccgc tggtcagata ccagttacca ctatttgccc cttgtccacg cgcgagagtg
    13081 tagtcatacg cccccgcaac gatacgacca gcctgaacaa attcgccctc agacttaccg
    13141 tctacatgga taagttcgat accgttaagg gttttcgcac ctgtaccgcc agcgttattc
    13201 accgtaacgg cagtcgtgcc gctagtatcg ccctcgacaa ccaatttatc ggttactgag
    13261 tcatcgccat tcagtaccgt attcattact agttgaccgt tattaccgtg gtagttcccc
    13321 ttcaccacta acacattgcc agcttgctgt cctgacattc cggtcatcag cgtaccgttg
    13381 ttggtcagat tgccgcccag ggtgtaattc acagacgcag ccgctctttt ttcagcgcta
    13441 ttattcaacg ccagggtacc gttgttctca acgtttgcgc tacccaatcc ctgcgcatga
    13501 cttaccagga cattagccga attagcaatg ttcacattcg ctgtcaggtt gctgttatcg
    13561 cccgcgatgt ccagcgtgcc gccctgaata ttcagctctc cgctccccgt taacgaaccg
    13621 gttgaagtgc ccccgttggt aaccgtcaga ctgccgccat ttaagctcag cagtgaatcg
    13681 gcgctgccat tgagcgtgcc cacggtctgg ctgtgaccat tcatatccag ttcagtctct
    13741 gccgccagac gcagttcatg ggtattaccc aacacgttat cgttattcaa caaaagcgtc
    13801 ccactgcgca gatcggtaac accggtatag tcgttgtctt tgttagaaag cgatacggtc
    13861 tgacccttct ggctgctgaa tgccaggtca ccactgccgg taatctttgc gctgagatcg
    13921 gcggcattct cgcttttacc gttagagctc agcaccagag catcgctgtc ggtagcgtgt
    13981 aaatcaagct gggtcaggcc gtaaccgata tacagaccgt cattgtttac cccactcgtc
    14041 agacgatagt cgtaagtgcc gacggccgct ttttgcccat tctgctgaat atcacgttgg
    14101 gcactgctgg agaggatttg cccattttta tcctgcaatt gcagattgcc cgcatcgccc
    14161 agaacttccg caccttgcgc atcaaccagc ttaatggcgc tgttaccatc atcgacttca
    14221 gtgagcgata acgcagagtc aatatcacga gatactgagc ggacaacgtc actgtcagaa
    14281 acctgaatca caccctcgcc gcgcaaatcc agcgttttac taacgttgac cgtcccttct
    14341 gtcatctgtg cacctgctgt cagggcacca aaatcaacgg ttccgccgga gaatcccaaa
    14401 gtgctaatgg agtgagcgcc cttacccact gttaccaggc ttccctgacc cgcagtgatc
    14461 ccgcctctct gcagaaactc agcattcttt tccaggttaa atgtcgtgtt ctggaacgcc
    14521 agtgaaccct ggaaccagtc tgtataagca aactcatcga aggagaattc gtgattcccc
    14581 atgtttattt caacaatacc tctaccttga agatcgttat caaataccca atctttattg
    14641 gcattgattt gcagtttgcc atcaacatcc aggtccgcac ggccgatgtt cttgtcttca
    14701 gaaataaccg cgacagcacc agaagcgaca tcaatatatc cgctgaatgc atagttattg
    14761 ccggtcagcg ccactgtccc actgtcgata cttattgtac cgttgccgga aatcttattg
    14821 gcaaaggtca tgctatcgtc agccttcagg tgtaatttcc cgcggttgtt gatttctgcg
    14881 gtacctatat tttgggtgat ccctaaagcg ccattttgct caattttgac tttaccgcta
    14941 aaggcggagt tatccccggt taagtaagtc gtaccattga gcgcattgat atagcccagg
    15001 ccctcgctgt cactgacaat atccgcggaa atagtcgcgt ttttatccgt gtggtttaag
    15061 gtgattttct tctcggatgt ctttgcccac acaaactcaa ttttttcggc atcaataatc
    15121 cctgcagcct tcgctgcgct tccttcctgt gcacctaacg ctaactcaga gttggtgctt
    15181 aaactaatag tcggcgcaga gattttacca ccttccgaaa cagcaagtgt tgtatcggag
    15241 ttttgatcat aataacccag gctaattttc tcactggcat taacttcacc gccgttatta
    15301 actgacaggt actgattacc accgtaactg ccgtaaacgc ccataaagaa ggttttagaa
    15361 tttacctggc tgccttcacc actgacattt ccctgcaaat aggtcagacg gctggtgccc
    15421 ttaacatgga ttttgccgcc gttggagaca ttaaaatagc cgcgatcgtg tctcatgcct
    15481 gagtttgact catacaaatc atcagcatat aactcgctgc cagcattgga gacattcagg
    15541 aaccccgt
//
LOCUS       DAZPDU010000098        15511 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZPDU010000098 DAZPDU010000000
VERSION     DAZPDU010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15511
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.099"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            202..474
                     /gene="asr"
                     /locus_tag="ACHURS_002754"
     CDS             202..474
                     /gene="asr"
                     /locus_tag="ACHURS_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFR1238436.1"
                     /translation="MGLSSAAFAAETATTPAPTATTTKAAPAKTTHHKKQHKAAPAQK
                     AQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA"
     gene            567..650
                     /locus_tag="ACHURS_002755"
     CDS             567..650
                     /locus_tag="ACHURS_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1238437.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            750..1571
                     /locus_tag="ACHURS_002756"
     CDS             750..1571
                     /locus_tag="ACHURS_002756"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFR1238438.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTNDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(1610..1939)
                     /gene="mdtI"
                     /locus_tag="ACHURS_002757"
     CDS             complement(1610..1939)
                     /gene="mdtI"
                     /locus_tag="ACHURS_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFR1238439.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(1926..2291)
                     /gene="mdtJ"
                     /locus_tag="ACHURS_002758"
     CDS             complement(1926..2291)
                     /gene="mdtJ"
                     /locus_tag="ACHURS_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFR1238440.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            2703..3737
                     /gene="tqsA"
                     /locus_tag="ACHURS_002759"
     CDS             2703..3737
                     /gene="tqsA"
                     /locus_tag="ACHURS_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFR1238441.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(3762..5150)
                     /gene="pntB"
                     /locus_tag="ACHURS_002760"
     CDS             complement(3762..5150)
                     /gene="pntB"
                     /locus_tag="ACHURS_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFR1238442.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPAAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(5161..6693)
                     /gene="pntA"
                     /locus_tag="ACHURS_002761"
     CDS             complement(5161..6693)
                     /gene="pntA"
                     /locus_tag="ACHURS_002761"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFR1238443.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            7217..8161
                     /gene="ydgH"
                     /locus_tag="ACHURS_002762"
     CDS             7217..8161
                     /gene="ydgH"
                     /locus_tag="ACHURS_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFR1238444.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEILGVATTA
                     SPSSEVGRFFETQSSKGGRYTVMLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            8347..9729
                     /locus_tag="ACHURS_002763"
     CDS             8347..9729
                     /locus_tag="ACHURS_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR1238445.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            9766..10488
                     /gene="folM"
                     /locus_tag="ACHURS_002764"
     CDS             9766..10488
                     /gene="folM"
                     /locus_tag="ACHURS_002764"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFR1238446.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSPHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(10485..10820)
                     /locus_tag="ACHURS_002765"
     CDS             complement(10485..10820)
                     /locus_tag="ACHURS_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFR1238447.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVSCWGISAWV
                     LIICWIKLH"
     gene            10949..11668
                     /gene="rstA"
                     /locus_tag="ACHURS_002766"
     CDS             10949..11668
                     /gene="rstA"
                     /locus_tag="ACHURS_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFR1238448.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            11672..12973
                     /gene="rstB"
                     /locus_tag="ACHURS_002767"
     CDS             11672..12973
                     /gene="rstB"
                     /locus_tag="ACHURS_002767"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFR1238449.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALITFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            13049..13978
                     /gene="tus"
                     /locus_tag="ACHURS_002768"
     CDS             13049..13978
                     /gene="tus"
                     /locus_tag="ACHURS_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFR1238450.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(13975..15378)
                     /gene="fumC"
                     /locus_tag="ACHURS_002769"
     CDS             complement(13975..15378)
                     /gene="fumC"
                     /locus_tag="ACHURS_002769"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFR1238451.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQVKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEQGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
BASE COUNT         3811 a         4021 c         3793 g         3886 t
ORIGIN      
        1 ggttatcgct cgccacggga atatctgcgg cagcgggctt gtaatgggtt aagtgataac
       61 agatgtctgg aaatataggg gcaaatccac tgccattccc agggatatag ttatttcaac
      121 ggccccgcgg tggggttaaa tgaaaaaaca aattgagggt atgacaatga aaaagtatta
      181 gctctggttg ttgccgctgc tatgggtctg tcttctgccg cctttgctgc agagactgcg
      241 accacacctg ctccgactgc gacgaccacc aaagcagcgc cggcgaaaac gacacatcat
      301 aaaaaacagc ataaagcagc acctgcccag aaagcgcagg cggctaaaaa gcatcataaa
      361 aatacgaaag ctgaacagaa agcccctgaa caaaaagcgc aggcagcgaa gaaacacgcc
      421 gggaaacaca gccatcagca accggcaaaa cctgctgcac aacccgcagc gtaagttttc
      481 aacagtaatg ctggcgcgcc ccctcgcgcc tgaaaattac ggtgctaagc gggtaacgtt
      541 tagcaccgcc tttagcggag ggtagtatgg tgggccgtta tcgctttgag ttcattctta
      601 tcatccttat tttatgcgca ctgattgccg cccgttttta tctttcctga ctgtagttat
      661 ctgattttac tcccactttc atcccgtccc gtctatagta tttatgaggg tttgctttta
      721 ataatcataa ttacccacca gagtgtgata tgcgtacaac cattgctgta gtgttgggtg
      781 caattagttt gacgtctgct tttgtgtttg cagataaacc agacgttgcc aaatcggcaa
      841 acgatgaggt cagcaccctg ttttttggtc atgatgatcg tgtgccagtg aatgacacga
      901 cccaatcacc gtgggatgcg gttgggcaac tggaaacggc cagcggcaat ttatgtacgg
      961 cgacgctgat tgcacccaat ctggcattaa cggcaggaca ctgtttattg acacctccaa
     1021 agggtaaagc cgataaagca gtggcgctgc gttttgtgtc aaataaaggt ctttggcgct
     1081 atgagatcca cgacatagaa ggccgcgttg atccgacact gggaaagcgg ttaaaagcag
     1141 atggggatgg ttggattgta cctcccgcag ccgcgccgtg ggacttcgga ttgattgtgc
     1201 tacgtaatcc cccttctggc attacaccgt tgccgttatt tgagggagat aaagccgcgc
     1261 ttactgccgc attaaaatcg gctggccgta aagtaactca ggcgggatac cctgaagatc
     1321 atctcgatac gttgtacagt catcaaaact gtgaagtgac tggctgggcg caaacgtcgg
     1381 tgatgtcaca tcagtgcgat accttgccgg gtgacagcgg ttcgcctctg atgttgcata
     1441 ccaatgacgg ctggcaatta attggggtgc aaagttcggc tcctgccgcg aaagatcgct
     1501 ggcgcgccga taaccgggcc atttctgtta ccggttttcg cgacaagctg gatcaactgt
     1561 cgcaaaaata atgttcaaac gctgcccgac tgtgcgggca gcgtgttcat caggcaagtt
     1621 tcaccatgat cattccagcc aacagcaaga ccaggccaat ccagccttta cgatttaacc
     1681 gttgaccaaa caagatccaa cctgcggcta acgtggcggc aataccaaac ccgccccaca
     1741 atgcataagc gacagacaag tcgatccctt taacggcctg agaaagcgca ctaaaggcag
     1801 ccagcaccgc cgccagggag agcaagccaa atattttgcg acgaaagccg tcagaaaatt
     1861 tcaaaaagac gttagcaacg atttccagca cgattgccaa tgccagccag gcggcgtgaa
     1921 cccattcaaa ctgcgccatg gttcacctcc agttcaggtt tacgcgcttt acgggtacct
     1981 gattttatca acacaatccc ggcgaccagg gtggttaacc cggcaatttt catcagcgat
     2041 aaactttcgt cgaataacaa aacgctaaac aaggtaataa ataaaatacc gataccttcc
     2101 cacagcgcat aagctacgcc taaggcgatc tttttaacgg cgaaagagag aaatatatac
     2161 gacagagaaa tcatcaccag cattaaaata aagccgccat ttccctcact gacgctcgcc
     2221 catttcattg acagcgtacc ggtaatttct gtagcaatag ccaggcctaa taaaatccaa
     2281 taaatataca ttgtccttct cctgcaagag aattatttta attttcgctt aattcagcga
     2341 aaccaaagta aagtggtcaa gccccgcgca tagggcaagc ttaggcagaa gaaaggacta
     2401 aagcgcgttg cgccagtgct gctcacctac gagcaagata gtggatgagg tacgaagatg
     2461 ggtaaatgtt gaaaataacg tcctgaacaa attgtccata atattacaat tatccgcagt
     2521 gttgcttctc gtcatcgcgg atgataattg tcctcggtag ttgaacacgc ctgatttgta
     2581 tcatagctta agaattaact caaaatattt tcacttcttt acctgagcgg tttgattttc
     2641 gttatgatga cggagcgaaa aagacattat tattagcaaa ggaagaaaaa acggggacaa
     2701 gcatggcaaa gccgatcatc acgctcaatg gcctaaaaat cgtcattatg ttgggaatgc
     2761 tggtcattat tctctgcggt atccgttttg ccgccgagat catcgtgccg tttattctcg
     2821 cattatttat tgctgttatt cttaacccgc tggtgcaaca catggtccgc tggcgagtgc
     2881 cgcgtgtact ggcggtgtcg attttgatga ccatcatcgt gatggcgatg gtgttgctat
     2941 tagcttatct gggttccgcg ctcaacgagt tgacgcggac gttaccgcaa tatcgcaact
     3001 ctattatgac gccgctgcaa gctcttgagc cgttgttgca acgcgtaggg attgacgtct
     3061 cagttgacca gctggcgcat tacattgatc cgaacgcggc gatgacgttg ctcaccaact
     3121 tattgacgca gttatctaat gccatgtcat caatattttt attgctgctg acggtgctgt
     3181 ttatgctgct cgaagtgcca caattgcccg gaaaatttca gcaaatgatg gcgcgtccgg
     3241 ttgaagggat ggcggcgatt caacgtgcaa ttgacagtgt ttctcattat ctggtgctga
     3301 aaacagccat cagcatcatc accggcctgg tcgcctgggc gatgctcgcc gcactcgatg
     3361 ttcgcttcgc ttttgtctgg ggattgctgg cctttgcgct taattacatc cctaatattg
     3421 gttcagttct cgcggcaatc ccccctatcg ctcaggtact ggtgtttaat ggcttctacg
     3481 aagcgttgct ggtgctggcg ggatatctgc tgattaatct ggtcttcggc aatattctgg
     3541 agccgcgcat catgggacgt gggctggggc tttccacatt ggtggtattt ttgtcgttga
     3601 ttttttgggg atggttgtta ggaccggtgg gtatgctgct ttccgtgccg ttaacaatta
     3661 ttgtcaaaat tgcgcttgaa caaacagcgg gaggtcaaag catcgccgtt ctgttaagcg
     3721 atctcaataa agagtgacgg cctccgcaga ggccgtcagg gttacagagc tttcaggatt
     3781 gcatccacgc tggctttggc gtcaccaaac agcatgtggg tgttttcctt gaagaacagc
     3841 gggttttgca caccagcata gccagtgttc atcgaacgtt taaagacaat cacgttctgc
     3901 gctttccaca cttccagcac aggcatacca gcaataggac tcttcggatc atcctgcgcc
     3961 gccgggttaa ccgtatcgtt agcaccaatc accagtacgg tatcggtatc agcaaagtca
     4021 tcgttgatct cgtccatttc cagcacgatg tcatacggta cttttgcttc agccagcaat
     4081 acgttcatat gtccaggcaa acgccccgca accgggtgga taccgaaacg cacgttgata
     4141 ccacgagcgc gcagtttctc ggtaatttca gcggcaggat attgcgcctg cgcgactgcc
     4201 atgccgtacc ccggagtaat gatcactgaa tgggagtttt tcagcagttc cgctgtctct
     4261 tctgcggtga tttcgcggtg ctcgcccact tcctgatcat cgccagtaga agagccgtcg
     4321 gtgccgaaac cacccgcaat aacgctgata aaggaacggt tcatcgcctt acacataatg
     4381 taagaaagga tagcccccga agaaccgacc agcgcaccgg tcacaatcag caggtcgttg
     4441 ctgagcataa agcccgcagc cgcagccgcc cagccggagt acgagttcag catcgacacc
     4501 accactggca tatctgcacc accgatggag gcgactaaat gccagccgaa taccagcgca
     4561 atggcggtca ttattagcaa tgccagcact tgcaggccga cgctgtccgt gcgaacaaat
     4621 acaatcagca gcaggaagga aacgaccaga gccgccaggt tcattttgtg acggtttggc
     4681 agcatcaacg gtttagacga aatcttgcca cacagtttgc cgaacgccac caccgaaccc
     4741 gtgaacgtta ccgccccgat gaagataccg aggaacactt ccgtcaggtg aatattgacc
     4801 agaatcggtg ccattcccgc gtcatgatgc agatagctgt taaagccaac cagcactgcc
     4861 gccagaccca cgaagctgtg caggatcgcc accagttctg gcatttcggt catttcaact
     4921 ttcttcgcca gacggatacc aattgcccca ccaatgacca tcgccagcaa gatccagcca
     4981 acattacccg tatccggtcc aaaaatggtt gcgattaacg caatcgccat cccggcgata
     5041 ccgaagttgt taccctggcg agacgtttca tgtttcgaaa gaccggccag actgaagata
     5101 aacaggatcg cggcaacaat gtatgcagct gtaactaatc ctccagacat atgttacccc
     5161 ttaatttttg cggaacattt tcagcatgcg ctgagtcacg gtgaagccac cgaaaatatt
     5221 aatgctggct ataagcaccg cgataaaact aaggaagcta acccagccgc cctggccaat
     5281 ctgcaacagt gctccgacaa caataatccc tgaaatcgcg ttggtgaccg acatcaacgg
     5341 tgtatgcagc gcgtgcgata cattccacac cacgtaataa ccgacaacgc aggccagcgc
     5401 gaaaacggtg aagtgcccaa gaaactcttt cggcgcaacg cttgccagcc aaccgaaaag
     5461 gatgattgcc agcgccatca acgcgtattt acgccacggt gagcaggcac atttttcctc
     5521 agttttcact tccggtgccg ctttttgtgc cgcctgcggc tgagctgata cctgaatcgg
     5581 cggtgccggc caggtaattt cgcccgcacg gatcacggtc acgccgcgaa tcaccacatc
     5641 atcaaaatca acagtgatat tgccgtcttt ctctttgcac aacagtttca gcagattaac
     5701 gaggttagta ccgtaaagct gcgaggattg cgtcggcaga cggcccggaa gatcggtata
     5761 accaatcact ttgacaccat tttccgtagt gaagatttca cccggcacgg tgtattcaca
     5821 gttgccaccg ttttgggctg ccaggtcgac aatcacactg cccgccttca tggagtcaac
     5881 catttcacgg gtaattagct tcggcgctgg tttgcctgga ataagcgcgg tggtgacaat
     5941 gatatcgacc tcttttgcct gggcggcaaa gagttccatt tccgctttaa tgaacgcgtc
     6001 cgacatcact ttggcatagc catcgccgct gcccgcttcc tctttaaaat ccagctcgag
     6061 gaattccgcg cccatacttt gaacttgttc tttcacttcc ggacgggtgt cgaatgcacg
     6121 cacaatcgcg ccgagactgt ttgctgcgcc aatggcggcc agacctgcaa cacccgcacc
     6181 aatcaccatc acttttgccg gtggcacttt cccggccgca gtaatttgcc cggtaaagaa
     6241 gcgcccaaat tcatgtgccg cttcaacaat ggcgcgataa ccggcgatgt tcgccatcga
     6301 gcttagtgcg tccagcgatt gtgcgcgtga gatacgcggc acagaatcca tcgccatcac
     6361 ggtcacgtta cgttccgcaa gtttttgcat taattccgga ttctgcgcag gccagataaa
     6421 actcaccagc gttgtcccag gattcagtaa cgcaatttca tcatctaacg gcgcattgac
     6481 cttcagaatg atctctgact gccagacgct attcccttct acaatttcag cgcccgcttg
     6541 cacaaacgct ttatcgtcaa aacttgccag ttgacccgcg ccgctctcta ccgcgacggt
     6601 aaaacccagt ttcagcagct gttccactgt ttttggcgtt gctgcaacac gggtttcatt
     6661 ggttaaccgt tctcttggta tgccaattcg catgatattc ccttccatcg gttttattga
     6721 tgatggtttg cctgtgtcag gagccacaca agccgctcat gtacgagcta aatgttactc
     6781 cgttaaaata aattagtaac aaacgcccta taacgtactg aaaattatgc ctgtgatcta
     6841 gcgccaaaaa tcagtatttc ggcgtgaact cgcaaaatat taacgattca gccgtgatag
     6901 tgggataaac accttagaac gccggataaa gactgataat tgtcttcgac ggtcgggtaa
     6961 aacgagacat cgccccggca cgattcacta cttaacatta aattaactta tacaattcag
     7021 ttgcttcagt agtaatgatg ctgatacggc tgttttttaa gcatagacgg tcatttgagc
     7081 aggattaaaa ttggcttaag gaatgtgata tgaaaaatga cgcagacagt tacaccgttt
     7141 aaatgcaata atcagccacg tttctcgtta ataacaatac cagtacctgg tttgcgcaag
     7201 gcgaaggatt atttttatga agcttaagaa caccctcctg gcgtcggcac tgctttctgc
     7261 tacggcattc tccgttaacg cagcaacaga actgacaccg gagcaagcgg cagcggttaa
     7321 accttttgac cgtgtagtgg ttaccggtcg ttttaatgct attggcgaag cggtgaaagc
     7381 cgtttctcgt cgcgcagata aagaaggtgc cgcctctttt tatgttgtcg acacttctga
     7441 ttttggtaac agcggtaact ggcgtgtggt cgctgacctc tataaagccg atgctgaaaa
     7501 agcagaagaa acaagtaatc gcgtaattaa cggtgttgtc gaactgccga aagatcaggc
     7561 tgttctgatt gaaccgtttg acacggtcac cgtccagggc ttctatcgta gccagccaga
     7621 agtcaatgat gccatcacca aagcggcaaa agcgaaaggt gcctactctt tctacatcgt
     7681 tcgtcaaatc gatgccaacc aaggcggcaa ccagcgtatt actgcattca tctataaaaa
     7741 agatgctaag aaacgtatcg tccagagccc ggatgtgatc ccggcagatt ccgaagcagg
     7801 acgtgcagct ctggctgccg gtggcgaagc cgcgaagaaa gttgagatcc taggtgttgc
     7861 gactaccgca tcaccaagtt ctgaagtcgg tcgtttcttt gaaacccagt catcaaaagg
     7921 cgggcgttac accgtcatgc tcccggatgg cactaaagtc gaagaactga acaaagcgac
     7981 cgcagcgatg atggtcccgt tcgacagcat caaattctct ggcaactacg gcaacatgac
     8041 cgaagtctcc tatcaggttg cgaaacgtgc cgcgaagaaa ggtgctaagt attaccacat
     8101 cacccgccag tggcaggaac gtggtaataa cctgaccgtc agcgcagatc tgtataaata
     8161 actattcagt tactcctata aggcggcttg atgccgcctt tcgcattttt agtcacaaat
     8221 ttccccgcga ccattgcatg cagacacatc tcttcgtaaa atcccgcgct tcagtggcaa
     8281 actccatttt tatgcgtttt gccctaatat ttattcttct tatcacgttt taatcactgg
     8341 atatcgatgg aaaagaaact gggactgagc gcactcaccg cgctggtatt aagctcaatg
     8401 ctgggcgcgg gtgttttcag tctgccgcaa aatatggcgg cagttgccag cccggcagca
     8461 ctgctcatcg gctggggtat tactggcgct ggcattttat tgctggcctt tgccatgctg
     8521 atcctcacgc gcattcgtcc cgaacttgac ggcggtatct ttacctatgc ccgcgaaggg
     8581 tttggcgagc taatcggctt ttgttccgca tggggatact ggctgtgcgc agtcatcgcc
     8641 aacgtctcct atctggtgat cgttttttcc gcgttaagct tttttacgga cacgccggaa
     8701 ttgcgcctgt tcggcgatgg taatacctgg caatcgatcg tcggtgcatc agctttattg
     8761 tggattgttc actttttgat cctgcgcggg gtgcaaaccg ctgccagcat taacctggtg
     8821 gcgacactgg caaaattgtt gccgctgggt ctgtttgttg tgctggcgat gatgatgttc
     8881 aaactggata ccttcaagct cgacttcacc ggacttgcac ttggcgtacc cgtttgggaa
     8941 caggtgaaaa acaccatgct gatcaccctg tgggttttca ttggtgtgga aggtgccgtg
     9001 gttgtttctg cgcgtgcgcg taataaacgt gatgttggca aagcgacact gctggcggtt
     9061 ctctccgctc tgggcgttta cttgctggta acgctgcttt cgctgggtgt ggtggcacgt
     9121 cctgaactgg ctgaaattcg taacccgtca atggccggtc tgatggtgga aatgatggga
     9181 ccatggggcg aaatcatcat cgctgccggt ttgatcgttt ccgtttgcgg cgcgtactta
     9241 agctggacca tcatggcggc agaagttccg ttcctggctg ccactcataa agcattccca
     9301 cgcatttttg cgcgccagaa cgcacaagct gcgccatccg cctcactgtg gttgaccaac
     9361 atctgcgtgc aaatttgtct ggtactcatc tggctgaccg gttccgatta caacacgttg
     9421 ctgaccatcg cctcagaaat gattctggtg ccctatttcc tggtcggcgc gttcttgctg
     9481 aaaatcgcaa cccgtccact gcataaagcg gtaggtgtcg gtgcctgcat ttatggctta
     9541 tggttattgt acgcctctgg tccaatgcac ttgctgttat ccgttgttct gtatgcgccg
     9601 gggctgctgg ttttcctgta tgcgcgcaaa acgcatacac atgataatgt actgaaccgt
     9661 caggaaatgg tgctgattgg catgctgtta attgcctcag ttccggcgac ctggatgctg
     9721 gtgggataac gttcatccca tcgttggata gcaaaggaga taacgatggg taaagcccag
     9781 cccttgccaa tattaattac tggcggaggt cgtcgcatcg gcctcgccct cgcctggcat
     9841 ttcattaatc aaaagcaacc ggtgattgtc agctatcgga cgcactatcc agccattgat
     9901 ggactgatta aggcaggtgc gcagtgtatt caggctgatt tttcgaccaa cgacggtgtg
     9961 atggcgtttg ccgatgaagt actaaaaagc ccccatggtc tgcgtgctat tttgcataac
    10021 gccagtgcgt ggatggcgga aaaaccgggt gcgccactgg ccgacgtact ggcttgcatg
    10081 atgcagatcc acgttaatac cccatacctg ctcaaccatg cgctggaaag attactgcgt
    10141 gggcacggac acgccgccag cgatatcatt cactttaccg attatgtggt ggagcgcggt
    10201 agcgacaagc atattgcgta tgctgcaagc aaagcggcac tggataatat gacccgctcg
    10261 tttgcccgca agctggcacc ggaagtgaaa gtgaactcta ttgctccatc gctgatcctg
    10321 tttaatgaac atgatgatgc cgaatatcga caacaggcgc tgaataaatc actgatgaaa
    10381 accgcgcctg gcgagaaaga agtgatcgac ctggtcgatt acttacttac cagttgcttt
    10441 gtcaccggac gcagtttccc acttgatggc ggtcgtcatc tgcgttaatg cagctttatc
    10501 cagcatataa tcaacaccca tgcgcttatc ccccaacacg aaacagaacc aacaaaagcg
    10561 gcgggcaagc gcatcattcc ggtgaaatac cacagcgaca ccaggtaaac aaaataggga
    10621 ataatcgacc acatactaaa aatgatggtt gcgcgtaagg cttcaatgcc gcgttcgctg
    10681 gcaacaatat aatgcgcgat aagcgcaaag gtcgggaaaa gtggaatcag cccggcgata
    10741 taataatttt tcgtttttgc taaaacacca atcaacagca ctaccagcgc accgagcgcg
    10801 gctttgatta ccagccccat ttttttacct taacacttcc ataacaagtc atcagtagaa
    10861 tacctgatga aaacttgttt agaaacgatt gatagtaagt aaaaacagcg cggtgtattg
    10921 tgacgttttt atatctaccg tgaatgttat gaacactatc gtatttgtgg aagatgatgc
    10981 ggaagtcggt tcactgattg ccgcgtacct ggcaaaacat gatatgcagg ttaccgtaga
    11041 gccgcgcggc gaccaggccg aagaaaccat tttgcgagaa aatccggatt tggtgttact
    11101 cgacatcatg ctaccaggca aggacggcat gaccatttgt cgtgatttac gcgcaaagtg
    11161 gtctggaccg attgttcttc taacctctct cgatagcgat atgaaccaca tcctggcact
    11221 ggaaatgggt gcctgcgact atattctcaa aacgacgccc cctgctgttt tgctagcgcg
    11281 tttacgtttg catttgcgtc agaatgagca agccacactg accaaaggtc ttcaggaaac
    11341 gtctctgact ccctacaaag ccctgcattt cggcacgttg accatcgatc ccatcaaccg
    11401 cgtagtcacc ctggctaaca ctgaaatctc gctctcgaca gctgatttcg aattattgtg
    11461 ggaattagct acccatgccg ggcaaatcat ggaccgcgat gcattgctga aaaatttacg
    11521 cggcgtcagt tatgacggac tggatcgtag cgtggacgtg gctatttcgc ggttaagaaa
    11581 aaaactgctc gataacgccg cagaacctta tcgcattaaa actgtgcgta acaaaggcta
    11641 tctttttgcg cctcatgcat gggaataagc gatgaaaaaa ctgtttatcc agttttacct
    11701 gttattgttt gtctgcttcc ttgtgatgtc tctgctggtt gggctggtgt acaaatttac
    11761 cgccgaacgc gcgggcaaac agtcgctgga tgatttgatg aacagttcac tgtatctgat
    11821 gcgcagcgaa ttgcgtgaga tccccccaca cgactggggt aagacgctga aagagatgga
    11881 tttaaatctc tctttcgatc tgcgtgtcga gccactgagt aaataccatc ttgatgatat
    11941 ttccatgcac cgactgcgtg gcggcgaaat tgtcgccctg gacgatcagt acacgttttt
    12001 gcagcgtatc ccgcgcagcc actacgtgct ggcagttggt cctgttcctt atctttatta
    12061 tctccatcag atgcgattgc tggatatcgc cctgatcact tttattgcta tttccctcgc
    12121 ctttccggtg tttatctgga tgcgtccgca ctggcaggat atgttaaaac tggaagcagc
    12181 ggcgcaacga tttggcgatg ggcatctcaa tgaacgtatc cactttgatg agggttcgag
    12241 ctttgaacga cttggtatcg catttaacca gatggcggac aatatcaacg ccttgattgc
    12301 cagcaaaaaa cagcttattg acggtatcgc tcacgaactg cgaacaccgt tagtgcgcct
    12361 gcgttatcga ctggagatga gcgataacct gagcgccgcc gaatcccagg cgttgaatcg
    12421 tgatatcagt caacttgaag ctttaattga agagctgctg acttatgccc gactcgatcg
    12481 cccacaaaac gagcttcatc ttagcgaacc agacctgccg ttgtggctgt caacgcatct
    12541 ggcagacatt caggcagtaa cgcccgataa aacggtacgg attaaaacgc tcgcgcaagg
    12601 ccattatgcg gcgttggata tgcgcttaat ggagcgcgtg ctggataatt tgctcaataa
    12661 cgccctgcgg tactgccatt caacggttga aaccagcctg ctactgtcgg ggaatagagc
    12721 gacattaatt gttgaggatg atggcccagg gattgcccca gaaaaccgcg aacatatctt
    12781 tgaacctttt gttcgcctcg atcccagccg ggatcgctca accggcggct gcgggctggg
    12841 gctggcaatt gtccactcta tagcactggc aatgggcggt acggttaatt gtgacaccag
    12901 cgaactgggt ggtgctcgct tctcgtttag ctggccgtta tggcataaca tcccgcaatt
    12961 tacctctgcc tgacactacg cgcacgatgg tcaagtcacc accactgtgc tataaaataa
    13021 gtatgttgta actaaagtgg ttaatattat ggcgcgttac gatctcgtag accgactcaa
    13081 cactaccttt cgccagatgg aacaagagct ggctgcattt gccgctcatc ttgagcaaca
    13141 caagctattg gttgcccgcg tgttctcttt gccggaggta aaaaaagagg atgagcataa
    13201 tccgcttaat cgtattgagg taaaacaaca tctcggcaac gacgcgcagt cgctggcgtt
    13261 gcgtcatttc cgccatttat ttattcaaca acagtccgaa aatcgcagca gcaaggccgc
    13321 tgtccgtctg cctggcgtgt tgtgttacca ggtcgataac ctttcgcaag cagcgttggt
    13381 cagtcatatt cagcacatca ataaactcaa gaccacgttc gagcatatcg tcacggttga
    13441 atcagaactc cccaccgcgg cacgttttga atgggtgcat cgtcatttgc cggggctgat
    13501 cacccttaat gcttaccgca cgctcaccgt tctgcacgac cccgccactt tacgctttgg
    13561 ttgggctaat aaacatatca ttaagaattt gcatcgtgat gaagtcctgg cacagctgga
    13621 aaaaagcctg aaatcaccac gcagtgtcgc accgtggacg cgcgaggagt ggcaaagaaa
    13681 actggagcga gagtatcagg atatcgctgc cctgccacag aacgcgaagt taaaaatcaa
    13741 acgtccggtg aaggtgcagc cgattgcccg cgtctggtac aaaggagatc aaaaacaagt
    13801 ccaacacgcc tgccctacac cgctgattgc actgattaat cgggataatg gcgcgggcgt
    13861 gccggacgtt ggtgagttgt taaattacga tgccgacaat gtgcagcacc gttataaacc
    13921 acaggcgcag ccgcttcgtt tgatcattcc acggctgcac ctgtatgttg cggattaacg
    13981 cccggctttc atactgccga ccatctgttc tggccgtacc cagctgtcaa actcggcttc
    14041 gctaagatac cccagcgcaa gggccgcagc ttttaaggtc agcccttctt tatgcgcttt
    14101 tttggcgatc tccgcggctt tgtcataacc aatgtgggtg ttaagcgcag tcaccagcat
    14161 cagcgattca ttgagtaatt gattgattcg ctcacgattc ggttcaatac ccactgcgca
    14221 gtgtttgtta aaactttcca tgccatctgc cagcaagcgc accgattgca ggaaattgtg
    14281 gatcaccatt ggacggaaga cgttcagttc aaagttaccg gaagcgccac ccatgttgat
    14341 cgccacgtcg ttccccatta cctgacagca gagcatggtt aatgcttcac actgggttgg
    14401 gttcactttc cccggcatga ttgagctgcc ctgctcattt tccgggattg agatttcacc
    14461 aattccgcag cgcgggccag aggccagcca gcggacatca ttggcgattt tcatcagtga
    14521 cgcagccaac cctttcaacg cgccgtgcgc ctgaaccagg gcatcacagg tcgcaagcgc
    14581 ttcaaatttg ttcggcgcgg taacaaacgg tgcacaggta atgactgcca gttcatctgc
    14641 tacgcgacgc gcatactccg gatgggtatt tagtccagta cccaccgctg taccgccgag
    14701 agccagttcc gctacgtgag gcaggctgta ttcgatatgt ttgagattat gctcgagcat
    14761 cgctacccag ccggaaatct cctgccccag tgttagcggc gtggcgtcct gcaagtgggt
    14821 tcgaccgatt ttgacgatat cggcaaatgc acgcgatttt tcactcagtg tctgtgtcag
    14881 ggttttaacc tgcggaatga gttgcttgcg cagcgccagc agcgccgcaa cgtgcatcgc
    14941 cgtcggaaag acatcgttgg aactttggct tttgttcacg tcgtcgttag ggtgaacttt
    15001 acgttccatc ccgcgcacgc cgccgagtaa ttcactggcc cggttagcca gcacttcgtt
    15061 catgttcatg ttactttgcg tgccggagcc ggtctgccag atagccagcg ggaattcgtc
    15121 gtcatgctgt cctgccagta cttcatccgc cgcctgccga atggcgctcg ctttctcttc
    15181 agacaacaag cctaaatctt cattaacttt tgccgctgcg cgcttggtta gcgccagcgc
    15241 atgaatcagt gaggtgggca ttttctccgt cgaaatgcgg aaatgctcca gcgagcgttg
    15301 agtttgtgcg ccccacagct tatctgccgg gacatcaatc gcccccatcg aatctttttc
    15361 gctgcgtact gtattcatgg cctgctcctc acctgattaa ttttttcttt ctgttttgcc
    15421 ttcgttaagc aacttttagc tcacttatta tttactattt gataacaaat gtttggtctt
    15481 tcgtgccatg taaaaaaacc gccccgaagg g
//
LOCUS       DAZPDU010000099        15444 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZPDU010000099 DAZPDU010000000
VERSION     DAZPDU010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15444
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.100"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(97..1209)
                     /gene="ydiK"
                     /locus_tag="ACHURS_002770"
     CDS             complement(97..1209)
                     /gene="ydiK"
                     /locus_tag="ACHURS_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFR1238452.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGSIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            1598..4654
                     /locus_tag="ACHURS_002771"
     CDS             1598..4654
                     /locus_tag="ACHURS_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFR1238453.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKTNTTIGRIYNTVY
                     QRCRLQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            4651..5061
                     /gene="menI"
                     /locus_tag="ACHURS_002772"
     CDS             4651..5061
                     /gene="menI"
                     /locus_tag="ACHURS_002772"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFR1238454.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            5161..5349
                     /locus_tag="ACHURS_002773"
     CDS             5161..5349
                     /locus_tag="ACHURS_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFR1238455.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSTELKEEIYFAWRLGVH"
     gene            5897..6265
                     /gene="sufA"
                     /locus_tag="ACHURS_002774"
     CDS             5897..6265
                     /gene="sufA"
                     /locus_tag="ACHURS_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFR1238456.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            6274..7761
                     /gene="sufB"
                     /locus_tag="ACHURS_002775"
     CDS             6274..7761
                     /gene="sufB"
                     /locus_tag="ACHURS_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFR1238457.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            7771..8517
                     /gene="sufC"
                     /locus_tag="ACHURS_002776"
     CDS             7771..8517
                     /gene="sufC"
                     /locus_tag="ACHURS_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFR1238458.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            8492..9763
                     /gene="sufD"
                     /locus_tag="ACHURS_002777"
     CDS             8492..9763
                     /gene="sufD"
                     /locus_tag="ACHURS_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFR1238459.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLAEDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKMAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            9760..10980
                     /gene="sufS"
                     /locus_tag="ACHURS_002778"
     CDS             9760..10980
                     /gene="sufS"
                     /locus_tag="ACHURS_002778"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFR1238460.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEIITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            10993..11409
                     /gene="sufE"
                     /locus_tag="ACHURS_002779"
     CDS             10993..11409
                     /gene="sufE"
                     /locus_tag="ACHURS_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFR1238461.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLTELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAHGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVSFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            11558..12562
                     /gene="ldtE"
                     /locus_tag="ACHURS_002780"
     CDS             11558..12562
                     /gene="ldtE"
                     /locus_tag="ACHURS_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFR1238462.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIVPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(12625..12861)
                     /gene="lpp"
                     /locus_tag="ACHURS_002781"
     CDS             complement(12625..12861)
                     /gene="lpp"
                     /locus_tag="ACHURS_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFR1238463.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(13171..14583)
                     /gene="pykF"
                     /locus_tag="ACHURS_002782"
     CDS             complement(13171..14583)
                     /gene="pykF"
                     /locus_tag="ACHURS_002782"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFR1238464.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(14591..14785)
                     /locus_tag="ACHURS_002783"
     CDS             complement(14591..14785)
                     /locus_tag="ACHURS_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1238465.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            15140..15349
                     /gene="fumD"
                     /locus_tag="ACHURS_002784"
     CDS             15140..15349
                     /gene="fumD"
                     /locus_tag="ACHURS_002784"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFR1238466.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT         3901 a         3738 c         4089 g         3716 t
ORIGIN      
        1 agctgaatgg cggtaagcag gaaaatatgg ggaagagcaa gcaaatatca attagcggtg
       61 attaacaatc aggcggcaga gccgcctgat gagaaattac ttattcggtt tctcgatttc
      121 gccaagttct tcgagaattt cttccggttg gtccgtcggc ggcgggactt cttccaccca
      181 cgcggcaaac agacgccagg aaacggctaa cagaaccgga ccaataaaca gaccgatcat
      241 cccgaaagca atcaaaccac caataacgcc agagaggatc aggatcagcg gtaaatcggc
      301 acccatgcga attaacattg ggcggatgac gttatccagc gtgccaacca caccgctcca
      361 cactaacaat accgttcccc aggtggtatc gccggtccag tagagccaga taatcgccgg
      421 aatcagtacc ggcaacgggc caagctggac aaggcaggag aggatcatta acaccgttag
      481 caacgttgca taaggtacgc cggatacggc gaggccgata ctgccaagca ctgcctgtac
      541 taacgccgtc accaccacac ccagcgccac cgcgcggata gcctgtgccg ccagcagcac
      601 ggcggcatct ccgcgaacgc ctgccagacg ggttgcaaaa tggcgaatgc cttgtgccac
      661 ctgttcaccg cgccagtaca gcagggcact gaagagaagc atcagcgcac aatgcaccat
      721 aaagcgcccg atatgcgccg cctgcccaac gaaccaggtg gtggtggtgc caatataagg
      781 gcggactttc gccatgatcg ccgtaccccc catatccagc aagttgtgcc agcctgcata
      841 cagcttcgcg ccaatcaccg gaatggtatt aagccacgcc agatcgggta acgtcatgtc
      901 gccgctggaa atggctttaa ttagcgggcc gctgccgtcg acgatactgt taaccagcaa
      961 agcaataggg atgataaaca ccatcactaa taacagcgtc atcaccagaa cggcgagaga
     1021 gcggcggcca aacatgatct tttgcaaacg taacaatacc ggccaggtgg cgataaccac
     1081 cgtaccggcc catgcaaagc cgagaataaa gggttgaaca atccacagac atgccacaat
     1141 catgatggct aaaaacagca ccgaaagcag aatttgtgcg acatccctgg gctgacgaac
     1201 atttaccata actacttttc accttttttg tgcgtcaaat cgtcgacgcg acgtgaacac
     1261 gcgaaactca cctgcataat gatgagcaat ttcagcggtt tttaacaggc cgattctgcc
     1321 cttaattctg atgggcgtgt tagaaaaaaa tgtggtacaa caactcaggc aacacgcaaa
     1381 cgatttactc gtcgtatttc aagttgcatg cgctgcggct acgttcgttc accccagtca
     1441 cttactattg taagctcctg gagttcactc gcttgccgcc ttcctgcaac tcgaaatcca
     1501 tagagtaaat aaacatcacc tttcagtgcg ctattgcagc cgcctgaaag aatagtgtat
     1561 cgacctacat cacagaccgc aggaaagggt caatataatg attccacaga tttcccaggc
     1621 acccggcgtc gttcaactgg tgcttaattt tttgcaagag ctggagcaac aaggttttac
     1681 cggcgatacg gcgacaagtt atgccgatcg tctgacaatg tcgaccgaca acagtattta
     1741 ccaacttctc cccgatgcgg tggtatttcc gcgttcaacc gcagatgtgg cgctgatcgc
     1801 ccgtcttgcc gcgcaggaac gctattcatc gctgatcttt accccccgcg gcggcggcac
     1861 cggcactaac ggtcaggcgc tcaaccaggg gattattgtt gatatgtccc gccatatgaa
     1921 ccgcatcatc gaaattaacc ctgaagaggg ctgggtgcgc gttgaggccg gggtgataaa
     1981 agatcaactc aatcagtacc tgaaaccgtt cggctacttt tttgcaccag aactttcgac
     2041 cagcaaccgg gcaacgctcg gcgggatgat caataccgat gcatccggtc agggatcgct
     2101 ggtctatggc aaaacgtcag atcacgtact tggcgtacgc gcggtgttgt tggggggcga
     2161 tattctcgat acgcaacctt tacccgtcga actggcggaa acgctgggta aaaccaatac
     2221 cacaatcggg cgaatttata acacggttta tcaacgttgc cgtctgcaac gccagttaat
     2281 catcgacaac ttccccaaac ttaaccgctt tcttaccggt tacgatctgc gtcatgtctt
     2341 taacgatgag atgaccgagt tcgacctgac gcgcattctg acgggttcag aagggacgct
     2401 ggcctttatt accgaagcgc ggctggatat tacgcccttg cctaaagtgc gccgtctggt
     2461 gaacgtcaaa tatgactctt ttgactccgc gctgcgtaac gcgccgttta tggttgaggc
     2521 gcgggcgctt tcggtagaga cggtggactc aaaagtgctg aatctggcgc gggaagatat
     2581 tgtctggcat tccgtcagcg agttgattac cgatgtgcct gacaaagaga tgctcgggct
     2641 gaacattgtg gaatttgctg gtgatgatga ggcgctgatt gacgagcgag ttaatgcact
     2701 ctgtgtgcgg cttgatgagc tgattgccag ccaacaggca ggtgtgattg gctggcaggt
     2761 gtgccgcgag ctggcggggg ttgaacgtat ctatgcaatg cgcaaaaaag ccgttggtct
     2821 gcttggcaat gccaaaggtg ccgctaagcc aattccgttt gctgaggata cctgcgtacc
     2881 gccggaacat ctggccgatt atattgctga atttcgcgcg ctgctcgaca gccacggctt
     2941 aagctacggt atgttcggtc acgtcgacgc aggtgttttg cacgtccgtc cggcactgga
     3001 tatgtgcgat ccgcaacaag agattttgat gaagcaaatc tctgatgacg tggtggcgct
     3061 gactgcgaaa tacggtggtt tgttgtgggg cgagcacggc aaaggttttc gcgctgaata
     3121 cagcccggcg tttttcggtg aggaactttt tgcagaactg cgcaaagtga aagcggcatt
     3181 tgacccgcat aaccgactca acccagggaa gatttgcccg ccagaaggtc tcgatgcgcc
     3241 gatgatgaaa gtggacgcgg tgaagcgcgg tacattcgat cggcagatcc ccattgcggt
     3301 acgccagcag tggcgcggtg cgatggagtg taacggcaac ggtttatgct tcaactttga
     3361 tgcccgtagt ccgatgtgtc cgtcgatgaa gatcacccag aaccggattc attcaccgaa
     3421 agggcgcgca acgctggtgc gtgaatggct gcgtttgttg gcggatcgcg gcgttgatcc
     3481 actcaaactg gaacaagaac tgcctgaatc cggcgtcagt ttacggacgt taattgcccg
     3541 cacgcgcaat agctggcatg cgaataaagg cgaatatgac ttctcacacg aagtcaaaga
     3601 ggcgatgtcg ggctgtctgg cctgtaaagc gtgttcgacc cagtgcccca tcaaaattga
     3661 tgtgccggag tttcgctctc gtttcctgca gctctatcac acccgttatt tacgcccgct
     3721 gcgcgaccac ctcgtcgcta cggtcgagag ctacgcaccg ctgatggcac gcgcgccgaa
     3781 aacctttaac ttcttcatta accagccgct ggtgcgcaaa ctctcggaaa aacatatcgg
     3841 catggttgat ttgccactgc tgtcggtccc ctcgctacaa caacaaatgg tggggcatcg
     3901 ctcggcaaac atgacgctgg aacagcttga agctctcaat gcagagcaga aagcgcgcac
     3961 ggtgttggtg gtgcaggacc cctttaccag ctattacgat gcgcaagtgg tggcggattt
     4021 tgtccgtctg gttgaaaaat taggtttcca gcctgtgtta ctgccatttt cgccaaatgg
     4081 caaagcccag catatcaaag gctttcttaa tcgttttgcg aagacggcga aaaagacggc
     4141 ggatttcctc aaccgtatgg ctaagcttgg gatgccaatg gtgggcgtcg atccggcgct
     4201 ggtactttgt tatcgcgatg aatataaact ggcgctgggc gaggaacgtg gcgagtttaa
     4261 cgtcttactt gcgaatgaat ggctggcaag cgcacttgag tcacatccag tggctacagt
     4321 cagcggtgaa tcatggtatt tctttggtca ctgtaccgag gttaccgctt tgccgggcgc
     4381 gccagcacaa tgggccgcga tatttgcccg ttttggcgcg aaactggaaa atgtcagcgt
     4441 gggttgctgc ggcatggcag ggacttacgg acatgaagcg aaaaaccatg aaaattcgct
     4501 cgggatctat gagttatcct ggcatcaggc tatgcagcga ctgccgcgta accgctgtct
     4561 ggcgaccgga tattcctgcc gtagccaggt aaaacgggtt gaaggcacgg gggtacgcca
     4621 tcctgtgcag gctttactgg agattattaa atgatatgga aacggaaaat caccctggaa
     4681 gcactgaatg ctatgggtga aggaaacatg gtggggttcc tggatattcg ctttgaacat
     4741 attggtgatg acacccttga agcgacaatg ccagtagact cgcggacaaa gcagcctttc
     4801 gggttgctgc atggaggagc atccgtggta ctggccgaaa gtatcggttc cgttgccggt
     4861 tatttatgta ccgaaggtga gcaaaaagtg gttggtctgg aaatcaatgc taatcacgtc
     4921 cgctcggcac gagaagggcg ggtgcgcggc atatgtaaac cgttgcatct cggttcgcgt
     4981 caccaggtct ggcagattga aatcttcgat gagaaagggc gtttgtgctg ttcgtcacga
     5041 ttgacgaccg ccattttgtg acgttgtcac cagaaaagtg tgacgcatat tgcttttggt
     5101 cattatctgc acagtaaacg gtggtatact gtgcacgtct tgtctaaaag cgaggatgct
     5161 atgtctactc aacttgatcc cacccagctg gccattgaat ttttacgccg tgaccagagc
     5221 aatctttcac ctgcccaata tctcaaacgt ctgaaacaac tagaacttga atttgctgac
     5281 ctgcttactc tctcttctac tgaattaaaa gaagaaatct actttgcctg gcgtctgggc
     5341 gtgcattgat cctgacttaa tgccgcaagt tcgccaaatc gacctggatt aacctccgtc
     5401 caggttcgat gaccttctta atactgctta tctaccgcct gatattgttc aaaaataata
     5461 ctaatcgcag tttgtgttaa aacggcgggt tagctttatg agattattct tatcgcccct
     5521 tcaagagcta agccactgag agtgccggag ataagcgccg gatggggtag aaacccttaa
     5581 gcctgtgtcg cacagactta agggttttct tatttctgaa cgctgcactt ttttacctat
     5641 ctcttaaata gtcattttca atacaacttt ttctttttca tcacagcctt aatgctcctt
     5701 agataataac cattatctaa caatgagata cctaattctt agcgtgcctg ttaacccaca
     5761 catcagggtc tatgcttatt aaaagcaggc aaaaagcatg ttttacggta aagcccctgc
     5821 gtttgctggg ttgaactgat aatcattatc actagcatgc tgttatacgc tgaaagcgat
     5881 gaagtgaggt aaatcgatgg acatgcattc aggaaccttt aacccacaag atttcgcctg
     5941 gcaaggctta acgctgacac ccgcagcggc gatacacatc cgtgagctgg tggcaaagca
     6001 gccgggtatg gtcggcgtgc gcttaggcgt gaagcaaacg ggctgcgcgg gctttggcta
     6061 tgtgctcgac agtgttagcg agccggacaa agacgatctg ctgtttgaac acgacggcgc
     6121 gaagctgttt gtcccgctgc aagcgatgcc gtttattgat ggcacggaag tcgatttcgt
     6181 tcgtgaagga cttaatcaga tattcaaatt tcacaaccct aaagcccaga atgaatgtgg
     6241 ctgtggcgaa agctttgggg tataggcggt actatgtctc gtaatactga agcaactgac
     6301 gatgtcaaaa cctggaccgg cggcccgctg aattataaag aaggattctt cacccagtta
     6361 gccaccgatg agctggcaaa ggggataaac gaagaggtgg tgcgcgcaat ttcggcgaag
     6421 cgtaatgagc cggagtggat gctggagttt cgtctaaacg cctatcgcgc atggctggag
     6481 atggaagaac cgcactggtt gaaagcgcac tacgacaagc tgaattatca ggattacagt
     6541 tactactcag caccatcgtg cggtaattgt gacgacactt gcgcgtctga acctggcgcg
     6601 gtgcagcaaa ctggcgcgaa cgccttttta agtaaagagg tggaggcggc gtttgagcag
     6661 ttgggcgttc ccgtgcggga aggcaaagag gtggcggtgg atgccatttt cgactctgtt
     6721 tcggttgcca ctacttatcg cgaaaaactg gcggagcagg gaattatttt ctgttccttt
     6781 ggtgaggcga tccacgatca tccggaactg gtgcgtaaat atctcggcac tgtggtgccg
     6841 ggaaatgaca acttctttgc cgcgcttaat gcggcggtag cctctgacgg tacgtttatt
     6901 tatgtgccta aaggcgtgcg ctgcccgatg gaactttcca cctattttcg cattaacgcg
     6961 gaaaaaaccg ggcagtttga gcgcaccatt ctggtggccg acgaagacag ctacgtcagc
     7021 tacattgaag gctgttccgc tccggtgcgt gacagctatc agttacacgc ggcggtggtg
     7081 gaagtcatca tccataaaaa cgccgaggtg aaatattcca cggtacaaaa ctggttccct
     7141 ggcgataaca acaccggcgg tattctcaat ttcgtcacca agcgtgcttt gtgcgaaggc
     7201 gaaaacagca aaatgtcatg gacgcaatca gaaaccgggt cagcgattac gtggaaatat
     7261 cccagctgca ttttgcgcgg cgataactcc attggtgagt tttactcagt ggcgctgacc
     7321 agcggtcatc agcaagcgga taccggcacc aagatgatcc acatcggtaa aaacaccaaa
     7381 tcgaccatta tctcgaaagg gatctctgcc ggacatagtc agaacagtta tcgcggctta
     7441 gtgaaaatca tgccgacggc aaccaatgcg cgcaatttca ctcagtgcga ctcaatgctg
     7501 attggcgcta attgtggggc gcataccttc ccgtatgtcg aatgtcgcaa taacagcgca
     7561 caactggagc acgaggcaac gacatcacgt attggtgaag atcaactgtt ttactgcctg
     7621 caacgcggga ttagcgaaga agacgccatc tcgatgattg ttaacggttt ctgcaaagac
     7681 gtgttctcag aactgccgct ggaatttgcc gttgaagcac aaaaactcct cgccatcagt
     7741 cttgaacaca gcgtcggata aggaataaac atgttaagta ttaaagattt acacgtcagc
     7801 gtggaagata aagctatcct gcgtggatta agcctcgacg ttcgtcccgg cgaagttcac
     7861 gccattatgg ggccaaacgg ttcgggcaaa agtaccttat cggcaacgct tgccgggcga
     7921 gaagattatg aagtgacggg cggcacggtt gagttcaaag gcaaagattt gcttgcgctg
     7981 tcgccggaag atcgtgcagg cgaaggcatc tttatggcct tccagtatcc ggtggagatt
     8041 cccggcgtca gtaaccagtt tttcctgcaa acggcactta atgcggtgcg cagctatcgc
     8101 ggccaggaaa cgctcgaccg ctttgatttt caggatttga tggaagagaa aatcgctctc
     8161 ctgaagatgc cggaagattt attaacccgt tcggtaaacg ttggtttttc cggcggcgag
     8221 aaaaagcgca acgatatttt gcaaatggcg gtgctggaac cggagttatg cattcttgat
     8281 gaatcggact ccgggctgga tattgacgca ttaaaagtgg ttgccgatgg cgtgaactcg
     8341 ctgcgtgacg gcaagcgctc attcatcatt gttacgcact accaacgcat tctcgactac
     8401 atcaagcctg attacgtcca tgtgctatat cagggacgaa ttgtgaaatc cggcgatttc
     8461 acgttggtca aacaactgga ggagcagggt tatggctggc ttaccgaaca gcagtaatgc
     8521 gctgcaacag tggcatcact tgtttgaagc tgaagggaca aaacgctcac cgcaagcaca
     8581 gcagcattta caacaattgc tgcgtaccgg actgccgaca cgtaaacatg aaaactggaa
     8641 atatacgccg ctggaagggc tgaccaatag tcagtttgtc agcattgcgg gagatatatc
     8701 cccacagcag cgtgatgcct tagcgttaac gttagacgcc gtgcggctgg tgtttgtcga
     8761 tggacgttac gtgcccgcgc tgagcgatgc aattgaaggc agcggatatg aagtgagcat
     8821 taacgacgac cgtcaggggt tacccgacgc tattcaggcg gaagtgtttc tgcatttgac
     8881 ggaaagcctg gcacaaagcg tgacgcatat cgccgtgaag cgcggtcaac ggccggcaaa
     8941 gccattgctg ttaatgcata tcacccaggg cgtggcaggt gaagaggtga acactgccca
     9001 ttaccggcat catctggatc tggcggaagg tgccgaagca acggtgatcg aacattttgt
     9061 cagccttaat gatgctcgtc attttaccgg ggcacggttc actatcaatg tcgcagcgaa
     9121 cgcccacttg cagcatatca agctggcgtt tgaaaacccg ctcagtcacc actttgctca
     9181 taacgatttg ttgctggctg aggatgccac cgcatttagc catagtttcc tgctgggtgg
     9241 cgcagtgtta cgacacaaca ccagtacgca actcaatggc gaaaacagca cgctgcggat
     9301 caatagcctg gcgatgccgg tgaaaaacga ggtgtgtgat acccgtacct ggctggaaca
     9361 caataaaggt ttttgtaaca gccgacagtt gcacaaaact atcgtcagcg acaaaggccg
     9421 cgcggtattt aacggtttga tcaacgtcgc gcagcacgcc atcaaaacgg atggtcagat
     9481 gaccaacaat aatctgctga tgggcaaaat ggcggaagtg gatacaaaac cgcagctgga
     9541 aatctatgca gatgatgtga aatgcagcca cggcgcgacg gtggggcgta ttgatgatga
     9601 acagatgttc tatctgcgct cgcgcgggat caatcagcag gatgcccagc agatgatcat
     9661 ttacgccttt gctgccgaac tgacggaagc actgcgtgat gaggggctta aacagcaggt
     9721 gctggcccga atcggtcaac ggctgccagg aggtgcaaga tgactttttc cgtcgacaaa
     9781 gtgcgggccg actttccggt gctttctcgt gaggtgaacg gtttgccgct ggcttatctc
     9841 gacagcgccg ccagtgcgca gaaaccaagc caggtgattg acgccgaggc tgagttttat
     9901 cgtcacggct acgcggcggt gcatcgcggt attcatacct taagcgccca ggcgaccgag
     9961 aaaatggaga acgtgcgcaa gcgggcatcg ctgtttatta atgcccgttc ggcggaagag
    10021 ctggtgttcg tccgcggcac gacggaaggg atcaatctgg tcgccaatag ttggggcaac
    10081 agcaacgtgc gggcgggcga taacatcatc atcagtcaga tggagcacca cgctaacatt
    10141 gttccctggc agatgctttg cgcacgcgtt ggcgcagagc tgcgtgtgat ccctctcaac
    10201 cccgacggta cgctgcaact ggagacgctg cctacgctgt ttgatgagaa aactcgcctg
    10261 ctggcaatta ctcatgtctc caacgtgctg ggaacagaaa atccgctggc ggaaataatc
    10321 acgcttgcgc accagcatgg cgcaaaagtg ctggtggatg gcgctcaggc ggtgatgcat
    10381 catccggtgg atgttcaggc gctggattgc gacttttacg tgttctccgg gcataaactg
    10441 tatggcccca ccggaattgg cattctttat gtgaaagaag ccttgttgca ggagatgccg
    10501 ccgtgggaag ggggcggttc tatgatcgcc accgtcagcc tgagtgaagg cactacctgg
    10561 accaaagcac catggcggtt tgaagccggt acacccaata ccgggggcat tattggtctt
    10621 ggcgcggcgc tggagtatgt ttcggcgctg gggcttaata acatagccga gtatgaacag
    10681 aatctgatgc attatgcgct atcacagctg gaatctgtac cggatctcac tctctatggc
    10741 ccacaaaaca ggcttggcgt tattgctttt aatctcggta aacaccacgc ctatgatgtt
    10801 ggcagttttc tcgataatta cggcattgct gtgcgtaccg gacatcactg cgctatgcca
    10861 ttaatggcct attacaacgt ccctgcgatg tgtcgggcgt cgctggccat gtataacacc
    10921 catgaagaag tggatcgtct ggtgaccggc ctgcaacgta ttcaccgttt gctgggataa
    10981 cagggaggca ctatggcttt attgccggat aaagaaaaat tgctgcgtaa ttttttacgc
    11041 tgcgccaact gggaagagaa atatctctac attattgagc tgggccagcg tctgactgaa
    11101 ttacgcgacg aagaccgaag cccacaaaat agcattcagg gctgccagag tcaggtgtgg
    11161 attgtcatgc gccagaatgc gcacggaatt attgaattgc agggcgacag cgatgcggcg
    11221 attgtgaaag ggcttattgc ggtagtcttt attctctacg atcagatgac gccgcaggat
    11281 attgtcagtt tcgatgtgcg tccgtggttt gaaaaaatgg cactcaccca acatctcacc
    11341 ccatctcgtt cacaaggtct ggaagcgatg attcgcgcaa ttcgcgccaa agccgctgca
    11401 cttagctaaa ctaaagggac agctttcatc cggttggctc acattgccgg atggtcaatg
    11461 tttacactga cctgcacagg agtgttttgg tttcaggtga acataagcgc catccgacgt
    11521 ttcagcgtga gtctccggca aatacaggag gtttacaatg aaacgcgcgt ctttgcttac
    11581 actcacgctt atcggcgctt ttagcgccat ccaggctgcc tgggcggttg attatccgct
    11641 accaccaacc ggaagccgac tggttgggca aaatcaaacg tatacggtgc aagaagggga
    11701 taaaaacctt caggctattg cccgacgttt tgatactgcg gcaatgttga tccttgaagc
    11761 caataacact atcgtcccgg tgccaaaacc tggtacgacg ataactattc cttcacaact
    11821 gttattacct gatgcaccgc gtcaggggat tatcgttaac cttgcagagc tgcgccttta
    11881 ttattatccg ccgggagaaa atattgtgca ggtttatcca ataggtattg gattgcaggg
    11941 gctggaaacg ccggtgatgg aaacgcgtgt tgggcagaaa atccctaacc caacctggac
    12001 gcctacggca ggcattcgtc agcgttcgct ggagcgtggc attaaattac cgccagtcgt
    12061 tcctgccgga ccaaataacc cgctaggacg ttacgcactg cgcctcgcgc atggtaatgg
    12121 cgaatacctc attcatggca ccagtgcgcc ggacagcgtt ggtttgcgcg tcagttcagg
    12181 gtgtattcgc atgaatgcac cggatattaa agccttgttc tccagcgtgc ggacgggaac
    12241 gccggtgaaa gtgatcaacg aaccggtgaa atattccgtg gaacctaacg ggatgcgtta
    12301 tgttgaagta catcgaccac tatcagcaga agaacagcaa aacgttcaga caatgccata
    12361 cacactgcca gcaggcttta cgcaatttaa agacaataag gctgtagatc agaagttagt
    12421 cgataaagcg ttgtatcgtc gggcagggta tccggtttcg gtgagcagtg gagcaactcc
    12481 cgcagccagc aatgcgcctt cagtagagtc agcgcagaat ggtgaaccag agcaagggaa
    12541 tatgttacgc gcgacgcagt agtggtaaat agcagacaaa aaaatggcgc acaatgtgcg
    12601 ccatttttca cttcacaggt actattactt gcggtattta gtagccatgt tgtccagacg
    12661 ctggttagca cgagctgcgt catctttagc agcctgaacg tcggaacgca ttgcgttcac
    12721 gtcgttgctc agctggtcaa ctttagcgtt cagagtctga acgtcagaag acagctgatc
    12781 gattttagcg ttgctggagc aacctgccag cagagtagaa cccaggatta ccgcgcccag
    12841 taccagttta gtagctttca ttattaatac cctctagatt gagttaatct ccatgtagcg
    12901 ttacaagtat tacacaaagt tttttatgtt gagaatattt ttttgatggg aaggcactta
    12961 tttttgatcg ttcgctcaaa gaagcatcga acgctgggtt ttagataaaa aaactgagag
    13021 aaaacaggat gcttccatcg gattcatctt agataaagtg agattatata gtgaaatccg
    13081 atttgatttt ttattgcttc tggttatcga ttaaataaaa aagcgcccat cagggcgctt
    13141 cgatatacaa attaattcac aaaagcaata ttacaggacg tgaacagatg cggtgttagt
    13201 agtgccgctc ggtaccagtg caccagaaac cataactacg acgtcacctt tgtgtgccag
    13261 accgctctgc agagccagtt ctttacccag acggtagaaa tcatcagtag aagtgatctc
    13321 tttaaccagc tgcggcacaa cgcctttgct cagtaccaac tgatgagccg ttttttcgtt
    13381 ggtggtcagt gccagtatgg tggcatccgg gaagtattta cgtactgcgc gagcagattt
    13441 accgccctgg gtagcaacca cgatcagcgg agcatccagt ttttcagcag tttcaacggc
    13501 accacggcat accgcttcgg taatgcgcag tttacggttg tcattgttga actcgagacg
    13561 gctgttcatc acgcggtcgg tacgttcgca gatggtcgcc atgatagaaa ccgcttccag
    13621 cgggtattta ccttttgcgg attcaccaga cagcatcact gcgtcagtac cgtcgaggat
    13681 ggcgtttgca acgtcaccgg cttctgcgcg agtcgggcgt gggtttttga tcatggaatc
    13741 cagcatctgg gtcgcagtga taacgacttt acgtgcacgg atacattttt cgatcatcat
    13801 cttctgggcg aagataactt cttctaccgg aatttctaca cccaggtcgc cacgcgcaac
    13861 catgatgccg tcagaggctt cgaggatttc gtcgaagttg ttgaggcctt cctggttttc
    13921 gattttggag atgatgtgga tgttttcgcc gccgtgcgct ttcaggtgct cacggatttc
    13981 gataacgtca gaacgcttac gaataaagga agcagcaaca aagtctacgc cttgttcgca
    14041 accaaagatc aggtcctgtt tgtctttttc agccagtgct ggcagggcaa tggaaacgcc
    14101 aggcaggttc acacctttgt tttcgcccag gtcgccgttg ttcagcactt tacagataac
    14161 tttgttacct tcaatggcag taacttccat accgatcaga ccatcgtcaa ccagtacggt
    14221 gttgccaaca gacaggtcag tagtgaaacc ttcatacgtt accgcaacca tttcgctgtt
    14281 gccgataaca gatttatcag tggtgaaagt aaaggtctga cccgctttca gagaaacgtc
    14341 gttaccgcct tccagtttca tggtgcggat ttccggacct ttggtatcaa gcaggatagc
    14401 ggcggtttta ccagttttgc tcatcacgtt gcgcagattc tgaatgcgct gaccgtgttc
    14461 tgcatagtca ccatgagaga agttcagacg cataacgttc atgccagcgt ccagcatttt
    14521 agctaacatc tcttcagatt cggttttcgg tccgatggtg caaacaattt tggtcttttt
    14581 catgacagtc ttagtcttta agttgagaag gatgggagaa acttgctttc tgggcgcact
    14641 gccggaaagc gaatccggta ttacgaaagt ggtggtgcct tgctctaagg ataggtgaca
    14701 tcgaaaaagc gtgcagagga atgtgtgctt gtgtctcagc ccaacgtgag tgcctgtttt
    14761 tgagtcgttg agttctacag tccaaagtgc tgaaaccatt caagagtcaa ttggcgcgca
    14821 ttatacgctg aaattaatcc aaaaggaaaa caaaaacagc gtttcaaaaa atatacaact
    14881 tgttagacaa taaattgtta tcaaagattt aacgttccag ggaaaaggtt acgcaaaaac
    14941 ttcgctgttt gcgcaacgtc tggtgccgaa gttgtcagtg tgaggataag tgaaagttca
    15001 tccggtgtat aaatatatca tcgctcaatt gctacagtta aaataattta tcgcgttatt
    15061 tgtctgattt tgtttgattt ttgtttaatt ggttagattg atatatgctg atagttggca
    15121 tgataaatag gtataaagaa tgggaaatcg gacaaaagag gatgaactgt accgagaaat
    15181 gtgcagagtt gtcggtaaag ttgtgctgga aatgcgcgat ctggggcagg aaccaaagca
    15241 tattgttatc gcaggcgtat tacgtactgc attagccaat aaacgcattc agcgcagtga
    15301 gctggaaaag caggcgatgg aaaccgtcat taacgcactg gtaaaatgac cattcaggat
    15361 gaggcggtaa gcttcatcct gatattcttg cttcagttgc ggtccagtta atcattctaa
    15421 ataacaactg ctgaaattat acga
//
LOCUS       DAZPDU010000100        15406 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZPDU010000100 DAZPDU010000000
VERSION     DAZPDU010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15406
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.101"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..453
                     /locus_tag="ACHURS_002785"
     CDS             <1..453
                     /locus_tag="ACHURS_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1238467.1"
                     /translation="GLCMDLALVTGQREGDLAAMRWEDIRDGRLYVEQQKTGAKIRIS
                     LPTTISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPP
                     PFHEIRSLSGRLHSAEKGGDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            669..758
                     /locus_tag="ACHURS_002786"
                     /pseudo
     CDS             669..758
                     /locus_tag="ACHURS_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
     gene            complement(759..1817)
                     /gene="modC"
                     /locus_tag="ACHURS_002787"
     CDS             complement(759..1817)
                     /gene="modC"
                     /locus_tag="ACHURS_002787"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFR1238468.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(1820..2509)
                     /gene="modB"
                     /locus_tag="ACHURS_002788"
     CDS             complement(1820..2509)
                     /gene="modB"
                     /locus_tag="ACHURS_002788"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFR1238469.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(2509..3282)
                     /gene="modA"
                     /locus_tag="ACHURS_002789"
     CDS             complement(2509..3282)
                     /gene="modA"
                     /locus_tag="ACHURS_002789"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR1238470.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFIIDSTTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVVSKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(3448..3597)
                     /gene="acrZ"
                     /locus_tag="ACHURS_002790"
     CDS             complement(3448..3597)
                     /gene="acrZ"
                     /locus_tag="ACHURS_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFR1238471.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            3726..4514
                     /gene="modE"
                     /locus_tag="ACHURS_002791"
     CDS             3726..4514
                     /gene="modE"
                     /locus_tag="ACHURS_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFR1238472.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDIQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            4582..6054
                     /gene="modF"
                     /locus_tag="ACHURS_002792"
     CDS             4582..6054
                     /gene="modF"
                     /locus_tag="ACHURS_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFR1238473.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     ESFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            6315..7331
                     /gene="galE"
                     /locus_tag="ACHURS_002793"
     CDS             6315..7331
                     /gene="galE"
                     /locus_tag="ACHURS_002793"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFR1238474.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            7341..8387
                     /gene="galT"
                     /locus_tag="ACHURS_002794"
     CDS             7341..8387
                     /gene="galT"
                     /locus_tag="ACHURS_002794"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFR1238475.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            8391..9539
                     /gene="galK"
                     /locus_tag="ACHURS_002795"
     CDS             8391..9539
                     /gene="galK"
                     /locus_tag="ACHURS_002795"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFR1238476.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            9533..10573
                     /gene="galM"
                     /locus_tag="ACHURS_002796"
     CDS             9533..10573
                     /gene="galM"
                     /locus_tag="ACHURS_002796"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFR1238477.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKSCPVNMTNHVYFNLDGEQSDVRNHKLQILAGEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            10776..11528
                     /gene="gpmA"
                     /locus_tag="ACHURS_002797"
     CDS             10776..11528
                     /gene="gpmA"
                     /locus_tag="ACHURS_002797"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFR1238478.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(11687..12739)
                     /gene="aroG"
                     /locus_tag="ACHURS_002798"
     CDS             complement(11687..12739)
                     /gene="aroG"
                     /locus_tag="ACHURS_002798"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFR1238479.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPVTENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            13055..13435
                     /locus_tag="ACHURS_002799"
     CDS             13055..13435
                     /locus_tag="ACHURS_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFR1238480.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQIGDGINNDVDTKTDGTTQ"
     gene            13549..14490
                     /gene="zitB"
                     /locus_tag="ACHURS_002800"
     CDS             13549..14490
                     /gene="zitB"
                     /locus_tag="ACHURS_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFR1238481.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSIVVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVITLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(14487..15206)
                     /gene="pnuC"
                     /locus_tag="ACHURS_002801"
     CDS             complement(14487..15206)
                     /gene="pnuC"
                     /locus_tag="ACHURS_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFR1238482.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(15244..>15406)
                     /locus_tag="ACHURS_002802"
     CDS             complement(15244..>15406)
                     /locus_tag="ACHURS_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="3 different subfamilies; catalyzes the formation of
                     quinolinate from iminoaspartate and dihydroxyacetone
                     phosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFR1238483.1"
                     /translation="CAHCPWMAMNGLQSIAEALEQEGSNHEVLDERLRERALVPLNRM
                     LDFAATLRG"
BASE COUNT         3916 a         4042 c         3851 g         3597 t
ORIGIN      
        1 ggcctttgca tggatcttgc actggtaaca ggacagcgcg aaggagatct ggcggctatg
       61 agatgggagg atataagaga tggcaggcta tacgtggaac aacaaaaaac aggggcaaaa
      121 attcgcattt ctctccccac cacgatttcc agattgaatt taacacttgc cgatgtactg
      181 gataacttga aaaaaataaa cggtaaaaac gaaaagttac tcggagggaa gacagccagg
      241 acaatcgcag cacagttcag gattgcccga gacacatccg gattgaaatg ggaaggtgac
      301 ccaccgccat ttcatgaaat ccgaagttta tccggtcgcc tgcactcagc agaaaaaggc
      361 ggtgatttca ctcaggcgct ccttggacat cgttcatcca gcatgacgga taaataccgt
      421 gacggacgag ggagggaatg gaaagacatt tagaattaat ttcgaatgat ttcgaaacac
      481 cacttgtaat taattgatta taaatgatta attcctaatg ggcatttgct tcatgaatgc
      541 tcctttgcat ttagcaactg actgtaacag ctaaaattag tcgcttttgg cggtgaatgc
      601 tgaattaaaa cgcagaattt tgtccgatag tggtatcggt gtaccattcg cgcagacgaa
      661 aattcttaaa tgacgcggta aaagcgcgcg ccaacattgt gattggtgat aacaccaccg
      721 acagcattgc ccagttcatt tatagccacc tgatttaatc aggcggttat cgacacactt
      781 ttaatttgcg cgtacagcca caggccaggt ttgatcgcca gttcatccct ggcccacggg
      841 ctgatacgcg cccacagcgt tttaccgccg acttccagct ccacttccac ctggccgttg
      901 tcgtcataac tattaacaac ttttgcccgc aatacgttac gaatgctggt ttgctgcggc
      961 ggttgtaaaa ccagagaaac atcggaagcc tgaatgcgga tacgtagcgc agcttgcagc
     1021 ggttcgtcca gcttattgac ccacaaatgc tgatcgccca gcgccagcgc ggtcatcgcg
     1081 taatgcggat gatgctcaag taccgtcact ttcagaatgc tactttgttg ctctttcggc
     1141 agccacggat tcatcacgct actgccccac acttcctcca gcgcgccaaa ggctttcacc
     1201 tgaccgtttt ccagtaccat cactctgtct gccagatgga ggatctcatc cagcgaatgg
     1261 ctgacataca acatcggaat gttgatttcc cgtgtcagcc gttgcagata aggcaacagt
     1321 tcgcgtttac gcggaatatc cagtgacgcc agcggttcat ccagcaacag caattccggt
     1381 gctgtcagca aagcccgacc aatcgccacg cgctgttttt cgcctccgga caggctgcct
     1441 ggtaaacggt caagcaacgg ttcaatgcct aaaagcgcca ccagcttatc gaactgatcg
     1501 accatacttt tcgacatgcc gtagcgcaga ttgccacgca ctttgtaatg cgggaacagc
     1561 cgcgcatcct gaaaaacata gccaacgcga cgcttttccg gcgtcaggca gatacctttt
     1621 tcggcatcat ttagtacccg cccattgagg acaatccgcc ctttttgcgg gcgcgtcagt
     1681 ccactgatgg cgttaatcag cgaagttttt ccggcaccgg agacgccaaa gatagcagtg
     1741 atgccattgg cgggcagtgt ttcattaatg gtcaggcaat ggttgcccaa cgtctgggaa
     1801 aaattcagct ccagcatgat tagcgccccg cccgttcacg gctgattctg gccagccatt
     1861 ctgaaatcaa cagggagatc atcgccagcg caatagaaat aatgcacagt ctcgccgcgc
     1921 cactttcccc gccgggggtc tggatcaggg tatacatggc agagggaatg gttcgcgttt
     1981 cgccaggaat gttcgacaca aaggtgatgg ttgcaccaaa ctcaccgaga gaacgagcaa
     2041 aagccagtac cgtaccaaca ataattcccg gtaaggtcag cggtaacgtg atagtaaaga
     2101 aaacgcgcca gcgcccggca cctagtgttc ttgcggcctg ttcgagtttg acatcaaccc
     2161 cttccagcgc cagacgaatt gcccgcacca tcagcggaaa cgacatgacg gcggcagcga
     2221 gaaccgcgcc gcgccagcta aaggcgaagg taataccaaa ccagtcatac agacgttcac
     2281 cgataaatcc gcgccgtccc atcgaaacta ataataagta accgacgacc acgggcggta
     2341 acaccagcgg tagatgcagt acgctgtcga gcagagcttt gcccggaaac gtgcaacgca
     2401 ccagtaacca ggcaaaaaag atcccaaacg gcaggctaaa cagcacagcc agggaagaaa
     2461 ctttcaggct taataaaact gcctgccatt ctggatcggt cagtatcatt acttggttgt
     2521 aaatccgtaa cgtttaaaga tttccgctgc ctgcggtccc ttcagataat cataaaatgc
     2581 tttcactgtc gcattgttat gcccttccac aacagcaacc ggatattcca cttttttatg
     2641 tgaatcttcc gggaaggtgg caaccacttt tacccctttg ctgacaactg cgtcagaacc
     2701 gtaaacaatg cccaacggcg cttcattgcg ttcgaccaac gccagcgcgc cacgaacatc
     2761 ttccgccgga gccagtttcg gggagagcgt atcccatgcg cccagttttt gcagtgcttc
     2821 ttttgcataa atgccagcag ggacatgttc cggatcgcca accgcgaggc gaccgccatt
     2881 cagcagcgaa gtccagttag ttgtgctgtc gatgatgaaa tctttctgct cgctggcttt
     2941 cggtgctaca acgaccaggc tattgccgag cagtgtctgg cgcgaagccg tatcgatcgc
     3001 ttttttatca accgcataat ccatccattt ctgatcggca gagataaaca gatccgcagg
     3061 cgctcccgct tcaatctgac gggccagagt agatgacgaa gcgaaagaag aaaccacatc
     3121 cacgcctttt tcttttttat actgcgtagc aatgtcctgc attgcgttag tcagtgatgc
     3181 ggcggcgaac accgtgattt tcccttcatc tgccagtgta ttgccagcaa cagcgaaaga
     3241 gagtgccgcc ccggcaaaca agttcaacca tttacgagcc attggtaacc ccttaatgta
     3301 acgttatata ggtgacaata taatggctag ctcagaattt tcccagcagt tattgctaac
     3361 ctttgtgagg tagataagaa aaaatatcgg ctggaaaagc aggaagttga gaaaaagaaa
     3421 tgcccgacta agcgggcatt cagggaatca atgattttgt ccgggctggt cttttttacc
     3481 aacaccagaa aagatgttga atacttcacc aagaccgtaa atcagaccca ggatgatggc
     3541 catcacgaca ggtaccatga ttacggcgaa taccagactt tttaataact ctaacatggt
     3601 caactccaga tatagtcatg agactattct aaccgctaag cacagaaaag cactcccctt
     3661 ttgtgcggtc agctttgcgt ggcgttcgtt ttccgtcaca ataagacttt tgccaggaca
     3721 ttgttatgca ggccgaaatc cttctcaccc ttaagctcca acaaaaatta ttcgccgacc
     3781 cgcgccggat ttcgctacta aaacacattg cgctttccgg ttccattagc cagggagcga
     3841 aagatgccgg tatcagctat aaaagcgcct gggatgccat taacgagatg aatcagttaa
     3901 gcgagcatat tctggttgag cgcgcaacag gcggtaaagg tggcggcggc gcagtactga
     3961 cccgctatgg tcagcgactg attcagctct atgacttact ggcgcaaatc cagcaaaaag
     4021 cctttgatgt gttaagtgac gatgacgccc tgccgctgaa cagcctgctg gccgcgatct
     4081 cacgtttttc actgcaaacc agcgcccgta accagtggtt cggtaccatc accgcccgcg
     4141 atcatgatga cattcaacag catgttgatg tcttactggc tgacggaaaa acacgcctga
     4201 aagtcgcaat taccgcacaa agcggcgcac gtctggggct ggatgaaggc aaagaagtgt
     4261 tgatattgct aaaagcgccg tgggtaggca ttactcagga cgaagcggtc gcacaaaacg
     4321 ccgacaacca attaccgggt attattagtc atattgagcg cggcgcagag cagtgcgaag
     4381 tattaatggc gctacccgac gggcaaacac tgtgcgccac agtgccggta aatgaagcga
     4441 cttctcttca gcaagggcag aatgtcacgg cctactttaa tgccgacagc gtgattatcg
     4501 ccacgctgtg ctaagcgtgt tgacaatttg ttatgaaaca cgtatccctg tcagtaatcg
     4561 ctgcacaaaa tgggatataa aatgtcatcg ttgcaaattt tgcaaggcac gtttcgtctt
     4621 agcgacacaa aaacgctgca attgcctcag ctaacgttaa acgcgggtga tagttgggcg
     4681 tttgtcggtt cgaatggaag cgggaaatcg gccctggccc gcgcgctggc gggggaactt
     4741 ccgcttttga aaggtgaacg gcaaagccag ttttcccaca tcactcgtct ctccttcgag
     4801 caattgcaaa aactcgtcag cgacgaatgg cagcggaata acaccgatat gctcggccct
     4861 ggcgaagatg acaccggacg cactacggct gaaatcattc aggatgaagt aaaggatgca
     4921 ccgcgttgca tgcaactggc gcagcagttc ggtattaccg ccctcctcga ccgacgcttt
     4981 aaataccttt ccactggcga gacgcgaaaa accctgctgt gtcaggcgct gatgtcggag
     5041 cctgacttgt tgattcttga tgagtcgttc gatggcctgg atgttgcctc acgtcagcag
     5101 ctggctgagc gactcgcctc gttacatcag tccggtatta ctctggtact ggtgctcaat
     5161 cgcttcgatg agatcccgga gtttgtccag tttgctggcg tgctggcgga ttgcacgtta
     5221 gcggaaactg gcgctaaaga ggaactgctc caacaagcac tcgtcgcgca actggcgcat
     5281 agtgaacagc ttgaaggtgt gcaactgccg gagccggatg aaccttcagc acgtcacgcc
     5341 ttacccgcca acgaaccgcg cattgtgctg aacaatggcg tggtttctta taacgatcgc
     5401 cccattctta ataaccttag ctggcaggtg aatccaggtg aacactggca aattgtcggg
     5461 ccaaatggtg caggaaaatc gacgttatta agcctggtta ctggcgatca tccgcaaggt
     5521 tacagcaacg atttgacgct tttcggacga cgtcgcggca gcggcgaaac catctgggat
     5581 atcaaaaagc atatcggtta cgtcagcagt agtttgcatc tggattaccg ggtcagcact
     5641 accgtgcgta atgtgattct ttctggctat tttgattcga ttggcattta tcaggccgtt
     5701 tcggatcgcc agcaaaaact ggtgcagcag tggctggata ttctcggcat tgataaacgt
     5761 acggctgacg ctccgttcca tagtctttcc tggggacagc agcgtctggc gctgatcgtc
     5821 cgcgcactgg tgaaacatcc gacgttgctt attctcgatg aaccactaca ggggcttgat
     5881 ccgctcaatc gccagcttat ccgccgtttt gttgatgtgc tgattagcga aggtgaaacg
     5941 caattgttat ttgtttcgca ccacgctgaa gatgcgcctg cctgtattac ccatcgcctt
     6001 gagttcgtgc cggacggtgg actctatcgc tatgtgctga caaaaatata ttgagtcggt
     6061 agtgctgacc ttgccggagg cggcctaagc gccctctccg gccaacggtt cgacgcatgc
     6121 aggcatgaaa ccgcttcttt tttcagataa aaagcgcaat cattcataaa ccctctgttt
     6181 tataatcact taatcgcgca taaaaaacgg ctaaattctt gtgtaaacga ttccactaat
     6241 ttattccatg tcacactttt cgcatctttg ttatgctatg gttatttcat accataagcc
     6301 taatggagcg aattatgaga gttctggtta ccggtggtag cggttacatt ggaagtcata
     6361 cctgcgtgca attactgcaa aacggtcatg atgtcatcat tcttgataac ctctgtaaca
     6421 gtaagcgcag cgtactgcct gttatcgagc gcttaggcgg caaacatcca acgtttgttg
     6481 aaggcgatat tcgtaacgaa gcgttgatga ccgagatact gcacgatcac gctatcgaca
     6541 ccgtgatcca cttcgccggg ctgaaagccg tcggcgaatc ggtacaaaaa ccgctggaat
     6601 attacgacaa caatgtcaac ggcactctgc gcctgattag cgccatgcgc gccgctaacg
     6661 tcaaaaactt tatttttagc tcctccgcca ccgtttatgg cgatcagccc aaaattccat
     6721 acgttgaaag cttcccgacc ggcacaccgc aaagccctta tggcaaaagc aaattgatgg
     6781 tggaacagat cctcaccgac ctgcaaaaag cccagccgga ctggagcatt gccctgctgc
     6841 gctacttcaa cccggttggc gcgcatccgt cgggcgatat gggcgaagat ccgcaaggca
     6901 ttccgaataa cctgatgcca tacatcgccc aggttgctgt aggccgtcgc gactcgctgg
     6961 cgatttttgg taacgattat ccgaccgaag atggtactgg cgtacgcgat tacatccacg
     7021 taatggatct ggcggacggt cacgtcgtgg cgatggaaaa actggcgaac aagccaggcg
     7081 tacacatcta caacctcggt gctggcgtag gcagcagcgt gctggacgtg gttaatgcct
     7141 tcagcaaagc ctgcggcaaa ccggttaact atcattttgc accgcgtcgc gaaggcgacc
     7201 ttccggccta ctgggcggac gccagcaaag ccgaccgtga actgaactgg cgcgtaacgc
     7261 gcacactcga tgaaatggcg caggacacct ggcactggca gtcacgccat ccacagggat
     7321 atcccgatta aggaacgacc atgacgcaat ttaatcccgt tgatcatcca catcgccgct
     7381 acaacccgct caccgggcaa tggattctgg tttcaccgca ccgcgctaag cgcccctggc
     7441 agggggcgca ggaaacgcca gccaaacagg tgttacctgc gcacgatcca gattgcttcc
     7501 tctgcgcagg taatgtgcgg gtgacaggcg ataaaaaccc cgattacacc gggacttacg
     7561 ttttcactaa tgactttgcg gccttgatgt ctgacacgcc agatgcgcca gaaagcaacg
     7621 atcctttgat gcgttgccag agcgcgcgcg gtaccagccg ggtgatctgc ttttcaccgg
     7681 atcacagtaa aacgttgcca gaactgagcg ttgcggcatt gacggaaatc gtcaaaacct
     7741 ggcaggagca aaccgcagag ctgggaaaaa cgtacccgtg ggtgcaggtc tttgaaaaca
     7801 aaggtgcggc gatgggctgc tctaacccgc atccgcacgg acagatttgg gcaaatagct
     7861 tcctgcctaa cgaagctgag cgcgaagacc gcctgcaaaa agaatatttt gccgaacaga
     7921 aatcaccaat gctggtggat tatgttcagc gcgagctggc agacggtagc cgtaccgttg
     7981 tcgaaaccga acactggtta gccgttgtgc cttactgggc tgcctggccg ttcgaaacgc
     8041 tactgctgcc caaagcccac gttttgcgga tcaccgattt gaccgacgcc cagcgcagcg
     8101 atttggcgct ggcgttgaaa aagctgacca gtcgttatga caacctcttc cagtgctcct
     8161 tcccctactc tatgggctgg cacggcgcac catttaatgg cgaagagaat caacactggc
     8221 agctgcacgc gcacttttat ccgcctctgc tgcgctccgc caccgtacgt aaatttatgg
     8281 ttggttatga aatgctggca gagacccagc gagacctgac cgcagaacag gcagcagagc
     8341 gtttgcgcgc agtcagcgat atccattttc gcgaatccgg agtgtaagaa atgagtctga
     8401 aagaaaaaac acaatctctg tttgccaacg catttggcta ccctgccact cacaccattc
     8461 aggcgcctgg ccgcgtgaat ttgattggtg aacacaccga ctacaacgac ggtttcgttc
     8521 tgccctgcgc gattgattat caaaccgtga tcagctgtgc accacgcgat gaccgtaaag
     8581 ttcgcgtgat ggcagccgat tatgaaaatc agctcgacga gttttccctc aatgcgccca
     8641 ttgtcgcgca tgaaaactat caatgggcga actacgttcg tggcgtggtg aaacatctgc
     8701 aactgcgtaa caacagcttc ggcggtgtgg acatggtgat cagcggcaat gtgccgcagg
     8761 gtgccgggtt aagttcttcc gcttcactgg aagtcgcggt cggaaccgta ttgcagcagc
     8821 tttatcatct gccgctggac ggcgcacaaa tcgcgcttaa cggtcaggaa gcagaaaacc
     8881 agtttgttgg ctgtaactgc gggatcatgg atcagctaat ttccgcactc ggcaagaaag
     8941 atcatgcctt gctgattgac tgccgctcac tggggactaa agcagtttcc atgccgaaag
     9001 gtgtggctgt cgtcatcatc aacagtaact tcaaacgtac cctggttggc agcgaataca
     9061 acacccgtcg tgaacagtgc gaaaccggtg cgcgtttctt ccagcagcca gctctgcgcg
     9121 atgtcaccat tgaagagttc aacgctgttg cacatgagct ggacccaatc gtggcgaaac
     9181 gcgtgcgtca tatcctgact gaaaacgccc gcaccgttga agctgccagc gcgctggagc
     9241 aaggcgatct gaaacgtatg ggcgagttga tggcggagtc tcatgcctct atgcgcgatg
     9301 atttcgaaat caccgtgccg caaattgaca ctctggtaga aatcgtcaaa gcagtgattg
     9361 gcgacaaagg tggcgtacgc atgaccggcg gcggatttgg cggctgtatc gtcgcgctga
     9421 tcccggaaga gctggtgcct gccgtacagc aagctgtcgc tgaacaatat gaagcaaaaa
     9481 caggtattaa agagactttt tacgtttgta aaccatcaca aggagcagga cagtgctgaa
     9541 cgaaactccc gcactggcac ccgatggtca gccgtaccga ctgttaactt tgcgtaacaa
     9601 cgcagggatg gtagtcacgc tgatggactg gggtgcgact ttactttcag ctcgtattcc
     9661 gctttccgat ggcagcgtcc gcgaggcgct gctcggctgt gccagcccgg aatgctatca
     9721 ggatcaggcc gcgtttctgg gggcctctat tggtcgttat gccaaccgca tcgccaatag
     9781 ccgttatacc tttgacggtg aaacggtgac gctttcgcca agtcagggcg ttaaccagct
     9841 gcacggcggc ccggaagggt tcgacaaacg tcgctggcag attgtgaacc agaacgatcg
     9901 tcaggtgctg tttgccctga gttcagatga tggcgatcag ggcttcccgg gtaatctcgg
     9961 cgcgacggtg caatatcgtc tgaccgacga taaccgtatc tccattactt atcgcgccac
    10021 agttgataaa tcttgcccgg tgaatatgac taatcacgtc tatttcaatc ttgacggcga
    10081 gcagtctgac gtgcgcaatc acaagttgca gattctggcg ggcgaatatc tgccggttga
    10141 tgaaggcggc attccgcacg acggcctgaa atctgtcgcc ggaacctctt ttgatttccg
    10201 cagcgccaaa atcatcgcca gtgagtttct tgccgacgac gatcagcgca aagtgaaagg
    10261 ttacgatcac gcattcttgt tacaggccaa aggcgatggc aagaaagtgg cggcgcatgt
    10321 ctggtcagca gatgaaaaat tgcagctgaa ggtctacacc accgctcccg ctcttcaatt
    10381 ctactccggc aacttcctcg gcggcacacc gtcgcgggga accgaacctt acgccgactg
    10441 gcaaggactg gcgttggaaa gcgagtttct gccggacagc ccgaaccacc ctgaatggcc
    10501 gcaaccggac tgcttcctgc gtcctggcga agagtattcc agcctgacgg aatatcagtt
    10561 tattgctgag taatgttaag ccctcgtcat gagggcttta tcttcatatt gttcaaatca
    10621 ccagcaaaca ccgacatatt tgcaactcaa tattcacaac aaccttacac tgcgccacta
    10681 ttttcgctat ggttatgcgt aagcattgct gttgcttcgt cacggcaata taatgagaat
    10741 tattatcata aaaagataat ttgaggagta agtatatggc tgtaactaag ctggttctgg
    10801 ttcgtcatgg cgaaagtcag tggaacaaag aaaaccgttt caccggttgg tacgacgtgg
    10861 atctgtctga gaaaggcgta agcgaagcaa aagcagcagg taagctgctg aaagaggaag
    10921 gttacagctt tgactttgct tacacttctg tgctgaaacg cgctatccat accctgtgga
    10981 atgtgctgga cgaactggat caggcatggc tgcccgttga gaaatcctgg aaactgaacg
    11041 aacgtcacta cggtgcgttg cagggtctga acaaagcgga aaccgccgaa aagtatggcg
    11101 acgagcaggt gaaacaatgg cgtcgtggtt ttgccgtgac tccgccggaa ctgactaaag
    11161 atgatgagcg ttatccgggt cacgatccgc gttacgcgaa actgagcgag aaagagctgc
    11221 cgctgactga aagcctggcg ctgaccattg accgcgtgat cccttactgg aatgaaacta
    11281 ttctaccgcg tatgaagagc ggtgagcgcg tgatcatcgc tgcacacggt aactctttac
    11341 gtgcgctggt gaaatatctt gataacatga gtgaagaaga gattcttgag ctgaatatcc
    11401 cgaccggcgt gccgctggtg tatgagttcg acgagaattt caaaccgctg aaacgctatt
    11461 atctgggtaa tgctgacgag atcgcagcga aagcagcggc ggttgctaac cagggtaaag
    11521 cgaagtaaac gtcattcgtt taaaaatgag aaagccgact gcaagtgagt cggctttttt
    11581 gttgctaaca atggagcaca ttgcctgatg cgacgctgcg cgtcttatca ggcctgtggt
    11641 gattcatcgg atacgccact ctgacggcgc atccgacaat taaaccttac ccgcgacgcg
    11701 cttttactgc attcgccagt tgacgtaaca gagcatcggt atcttcccag ccaatgcagg
    11761 catcggtgat gctcttaccg taggccagcg gctccccgct atcgaggctc tgattgcctt
    11821 ccaccagatg gctttccacc atcacgccaa taatggcctt ttcgccaccg gcaatctgct
    11881 ggcaaacgtc agcacaaaca tccatctgct ttttgaattg tttggacgag ttagcatggc
    11941 tgaaatcgat catcacctgt gctggcaggc ctgctttgtt cagcccttct ttcacttcag
    12001 caacgtgctt cgcgctgtag ttaggctctt taccgccgcg cagaatgata tggcaatcac
    12061 cgttaccgct ggtattcaca atcgccgaat gcccccattt agttacggac aggaagcagt
    12121 gcggcgcacc ggcggcatta atggcatcga tagccacttt aatcgtaccg tctgtgccat
    12181 ttttaaaacc taccggacaa gaaagaccag acgccagttc gcggtgcacc tgcgattccg
    12241 tggtacgtgc accaattgcg ccccagctca tcaggtcagc gagatactga ggagtgatca
    12301 tatcgagaaa ctcacccgcc gctggcagac cgctgtcgtt aatatcaagc agcaatttac
    12361 gggctatacg cagaccgtcg ttgatctgga agctgttatc catatgcgga tcgttaatca
    12421 accctttcca gcccaccgtg gtacgcggct tttcaaaata gacgcgcatt acgatttcca
    12481 gctcatcttt cagctcttca cgcagcgcca gcaagcgagt ggcatactct ttagccgcga
    12541 caggatcatg aattgagcac gggccaatca ccaccaacag gcgatcatca ttacctttca
    12601 ggatcttatg gatcgctttt cgggcatggg caaccgtatt cgcggcattt tcagttacgg
    12661 ggaatttttc cagcaatgcg acaggaggaa gtaactcttt gatttctttg atgcgtaaat
    12721 cgtcgttctg ataattcatg tctgttccag tgttgccata cttatcttag tgaatgcaat
    12781 acttccaatc tatatcttcc gtcagaatgt gtaaacgggg ttttacacta tgaacggatt
    12841 aatcctggaa ttagcaagaa aaacgccaga ttgtcgcgaa aaacgagatc tctcctacaa
    12901 tttctaactg taactccttt gtttgttaat tatttcaaga ttctctgctg cgtttcataa
    12961 actggctgaa atcttaaacc aatgccttat attcacctgc aaatgcactg ttggaagagg
    13021 ttatccgaca taacgaccat aacaggagca tcctatgaaa atgacaaaac tggccacatt
    13081 atttctgact gccactctaa gccttgccag cggtgccgca ctggctgccg atagcggagc
    13141 gcaaactaat aacggccagg caaacgccgc agctgatgcg ggccaggtag cccctgacgc
    13201 ccgtgaaaat gtcgcgccaa ataacgtcga caataacggg gtaaataccg gttctggcgg
    13261 cacaatgctg cattcggatg gttcttcaat gaacaatgac ggaatgacca aagatgaaga
    13321 gcacaagaac accatgtgca aagatggtcg atgtccggat atcaataaaa aggtacaaat
    13381 cggtgatggc atcaataatg atgtcgatac caaaaccgac ggtaccacgc agtaaccctg
    13441 caaaatagcc aataatggga gagcggagtc tctccctttt aatttttcac ttctaaaagc
    13501 agtatgatgt cataaagtta agtgagaata attaagggat gactcgtcat ggcgcactca
    13561 cactcacaca cgtcctcaca cctgccagaa gataataatg ctcgtcgctt gttgtatgct
    13621 ttcggcgtga ctgccgggtt tatgctggta gaagtcgttg gtggttttct ttctggttct
    13681 ctggcattgc tggccgatgc gggtcatatg ttgaccgata ctgccgccct gcttttcgcc
    13741 ctactcgccg tgcaattttc acgtcgtcct cccaccattc gccacacttt cggttggcta
    13801 agactgacca cgctcgcggc gtttgtgaat gccatcgccc tcgtggtaat taccattttg
    13861 attgtctggg aggcgataga acgcttccgc acgccgcgtc cggtcgaggg cggcatgatg
    13921 atggcaattg ccgtggctgg gttgctggca aatatacttt ctttctggtt acttcatcac
    13981 ggcagcgaag agaaaaacct caacgtacga gcagcggcat tgcatgtgct cggggacctg
    14041 ttgggttcgg ttggggctat tattgccgcg ttgattatta tctggaccgg ctggacacct
    14101 gctgacccca ttctctcgat agtggtgtcg cttctggttc tgcgtagcgc gtggcgactg
    14161 ttgaaagata gcgtgaatga attacttgaa ggtgcaccgg tatcgctgga tatcgctgaa
    14221 ctgaagcgtc gtatgtgccg ggaaatcccg gaagtccgca atgtgcacca tgtacatgta
    14281 tggatggtag gcgagaagcc ggtgataacg ctgcatgtgc aggtgatccc gccacacgat
    14341 cacgatgcct tgttggatca gatccaacac tacctgatgg atcactatca gattgagcac
    14401 gccaccattc agatggaata tcaaccttgt catgggccag actgccacct gaatgaaggg
    14461 gtgtctggcc attcacatca tcaccattaa tgggacagcg cgcgtgagcc tctttcacgt
    14521 gcgctgttga tccacatccg actgccgttg agcgcaataa aggtcaggat gatgtactcc
    14581 agagacatgg cgtaaacgcc ctgaagtgca aaaataacga cgctaatcac gttaataatc
    14641 acccacaaca gccagttttc cacatactta cgcgtcatca gaatcattgc cacgatagat
    14701 aacaccatca tgcatgaatc ccagaacggg aaagcgtccg gttgcagttc aggcatcaca
    14761 acctgtaatc ctaatgcttg catgatcatg actgccacgc gggtcaaaaa tgcaaacacc
    14821 ggattgataa ataccgtcat cagaccaatc gaaacaacgc aaaccgccaa ccagctgagc
    14881 gccttcggca atggtaacca gcgaattttc aactccgcct cgttctgact ggtttgtcgc
    14941 gaccacgcat accaaccgta aatattcgcg gcaaagaaaa acacctgtaa tagcaggctg
    15001 gcatacagct gaatctgaaa gaaaataatg ccaaacaagg tgacgttgat caggccaaag
    15061 aagtagttgc tgatcttctc cagactggca aggccaatac acagcaaccc ggcgatcgtg
    15121 cctaccgctt cgatccatga gagatcataa ccgcctgccc ctattggtat atgtaccagg
    15181 atattctgca cactaaaaaa atccatcttt cccccaaagc gtaacgttac gccttattat
    15241 tcgttatcca cgtagtgtag ccgcaaaatc cagcatacga ttgagcggca ccagcgccct
    15301 ctctcgcagc ctttcatcaa gaacctcgtg attgcttcct tcctgttcta atgcctctgc
    15361 gatggactga aggccattca tggccatcca cggacaatgc gcgcag
//
LOCUS       DAZPDU010000101        15349 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZPDU010000101 DAZPDU010000000
VERSION     DAZPDU010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15349
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.102"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..1015
                     /locus_tag="ACHURS_002803"
     CDS             <1..1015
                     /locus_tag="ACHURS_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1238484.1"
                     /translation="KISSDGCLKKEQMVVSAIASTPQLPMLGEKRLVSSVTKEDLLFV
                     RRDLLTGYQKLSNGKISSIKGRSVVTVNYYMTTIAGMFQFATDNGYTSGNPFNGLTPL
                     KKSKIEPDPLTRDEFIRFIEACRHQQTKNLWIIAVYTGIRHGELVSLAWEDIDLKART
                     ITIRRNYTKLGEFTPPKTDAGTGRTIHLVQPAIDALKSQAEMTMLGKQHSVEVKQREY
                     GRSTVHKCTFVFSPQVIKQRQFSGPHYKVDSIRESWTSILKRAGLRHRKSYQSRHTYA
                     CWSLAAGANPSFIASQMGHTNAQMVFNVYGAWMKDNNHEQIELLNKRLSESVPCMPHK
                     KVG"
     gene            1051..1737
                     /gene="mlrA"
                     /locus_tag="ACHURS_002804"
     CDS             1051..1737
                     /gene="mlrA"
                     /locus_tag="ACHURS_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFR1238485.1"
                     /translation="MPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIKRWIDN
                     GVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYPAQTLT
                     THLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKKQGKDALVVGWNIQDT
                     TRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQLTSWQE
                     QGHDIFPLGI"
     gene            1797..1904
                     /locus_tag="ACHURS_002805"
     CDS             1797..1904
                     /locus_tag="ACHURS_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFR1238486.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(1885..2616)
                     /gene="yehW"
                     /locus_tag="ACHURS_002806"
     CDS             complement(1885..2616)
                     /gene="yehW"
                     /locus_tag="ACHURS_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFR1238487.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(2621..3547)
                     /gene="yehX"
                     /locus_tag="ACHURS_002807"
     CDS             complement(2621..3547)
                     /gene="yehX"
                     /locus_tag="ACHURS_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFR1238488.1"
                     /translation="MIEFGHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLEEA"
     gene            complement(3540..4697)
                     /gene="yehY"
                     /locus_tag="ACHURS_002808"
     CDS             complement(3540..4697)
                     /gene="yehY"
                     /locus_tag="ACHURS_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFR1238489.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGTLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(4704..5621)
                     /gene="osmF"
                     /locus_tag="ACHURS_002809"
     CDS             complement(4704..5621)
                     /gene="osmF"
                     /locus_tag="ACHURS_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFR1238490.1"
                     /translation="MPLSKVWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPKGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(5873..8170)
                     /gene="bglX"
                     /locus_tag="ACHURS_002810"
     CDS             complement(5873..8170)
                     /gene="bglX"
                     /locus_tag="ACHURS_002810"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFR1238491.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            8366..10081
                     /gene="dld"
                     /locus_tag="ACHURS_002811"
     CDS             8366..10081
                     /gene="dld"
                     /locus_tag="ACHURS_002811"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFR1238492.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYIHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIGSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(10120..11052)
                     /gene="pbpG"
                     /locus_tag="ACHURS_002812"
     CDS             complement(10120..11052)
                     /gene="pbpG"
                     /locus_tag="ACHURS_002812"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFR1238493.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(11226..11813)
                     /locus_tag="ACHURS_002813"
     CDS             complement(11226..11813)
                     /locus_tag="ACHURS_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFR1238494.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            11983..12561
                     /locus_tag="ACHURS_002814"
     CDS             11983..12561
                     /locus_tag="ACHURS_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR1238495.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(12691..13452)
                     /locus_tag="ACHURS_002815"
     CDS             complement(12691..13452)
                     /locus_tag="ACHURS_002815"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR1238496.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLELGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKTMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(13505..14941)
                     /gene="mdtQ"
                     /locus_tag="ACHURS_002816"
     CDS             complement(13505..14941)
                     /gene="mdtQ"
                     /locus_tag="ACHURS_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFR1238497.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLNIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            15165..15248
                     /gene="yohP"
                     /locus_tag="ACHURS_002817"
     CDS             15165..15248
                     /gene="yohP"
                     /locus_tag="ACHURS_002817"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFR1238498.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT         3746 a         4180 c         3840 g         3583 t
ORIGIN      
        1 aaaaatatca tcagatggct gcttaaaaaa agaacaaatg gtagtgtccg ctattgccag
       61 tacacctcag ttgccaatgt taggtgaaaa aagactggtt tcatctgtaa caaaagagga
      121 tttgcttttc gtaaggagag atctgttgac cggttatcaa aagctttcta acggaaaaat
      181 ttcttccata aaaggacgct cagtggtcac agtaaactac tatatgacaa ccatagctgg
      241 aatgtttcaa tttgcaacag ataatggtta tacatcagga aatccattta acggtctgac
      301 accattaaaa aagtccaaga tagaaccaga tcccctcacc cgtgacgaat ttattcgttt
      361 tattgaggct tgccgtcatc aacaaacaaa aaacctgtgg attatcgctg tatacacggg
      421 tattcgtcac ggggagctgg tatcactggc ctgggaagat atagacctta aagcgaggac
      481 tataaccatc cgccggaatt atacaaaact aggcgaattc actccaccaa aaactgatgc
      541 tggtaccgga agaacgattc atctagttca accagctatt gatgctctta aaagccaagc
      601 ggaaatgacc atgcttggaa aacagcattc tgtagaggta aagcagaggg aatatgggag
      661 aagtactgtg cataaatgca cttttgtttt tagtcctcag gtaataaaac agcggcagtt
      721 ttccggaccg cactataagg ttgactccat cagggagtca tggacaagta tcttaaaacg
      781 cgcaggtctg agacacagaa aatcgtacca atccaggcat acttatgcat gctggtcact
      841 tgccgctgga gctaatccta gttttatcgc aagccagatg ggccacacaa acgcacaaat
      901 ggtattcaat gtttacggag catggatgaa agacaacaat cacgaacaga tagaactcct
      961 taacaaaaga ctatctgaaa gtgtcccatg tatgccccat aagaaagtgg ggtaaaataa
     1021 aaacttgtaa aatcagttag tttaccctta atccctgtca cgttacgcgc gtggcagagg
     1081 cgttacgggt tgctgaaacc gcaacggaca gacggcggtc atcgactgtt caacgatgcc
     1141 gatattgacc ggatccgcga gatcaaacgc tggatcgaca acggcgtgca ggtcagcaaa
     1201 gttaaaatgc tgctcagtaa tgaaaatgtt gatgtgcaga acggctggcg cgatcagcaa
     1261 gaaacattac tgacctacct gcaaagcggc aatctgcata gcctgcgaac gtggatcaaa
     1321 gagcgcggtc aggattaccc cgcccagaca ctcaccacac atctgtttat tcctctgcgc
     1381 cgacggcttc agtgccaaca accgactctc caggcgctgc tggcgatcct cgacggcgta
     1441 ctgatcaact acatcgccat ttgtctggct tcggcacgta aaaaacaggg taaagatgcg
     1501 ctggtggttg gctggaatat tcaggatacc acccgtctgt ggctggaggg ctggattgcc
     1561 agtcaacaag gatggcgcat tgatgtcctc gcccactcgc tcaatcaact acgccctgaa
     1621 ctattcgaag gccgtacatt gctggtgtgg tgcggtgaaa atcgaacctc cgcccaacag
     1681 cagcaactca ccagttggca agaacaaggc catgatattt tcccactcgg catttaatga
     1741 ttcgttaaca aatgcgcttt actgtacaat cctttcgtta acataaggag tgcaatatgc
     1801 gcatagctaa aattggggtc atcgccctgt tcctgtttat ggcgttaggc ggaattggtg
     1861 gcgtcatgct cgcaggttat acctttattt tgcgtgctgg ctaagcgcct gcaccagcct
     1921 ttcaaacagg cggtctgcga tgatcgccgc cagtgccacc agtaacgccc cctggatcac
     1981 atacgcggta ttaaatccgc taagcccgat gatgatgggc gtacccagcg tgctggcccc
     2041 taccgttgag gcgatcgtcg ccgtaccaat gttgataatc accgaagttc gcacgcccgc
     2101 cagaatcacc ggagccgcca gcggtagctc gaccttacgc agtcgctgac cacgactcat
     2161 tcccatacct ttcgcaactt ctgtcacgct ggcatcaatc gctcccagcc cggcaagtgt
     2221 cgcctgcagg acgggcagca caccgtaaag gatcaaggcg ataatcgctg gttgcagacc
     2281 aaagccgatc accggaacgg caatcgccag cactgcgaca ggcggaaaag tctgtccaac
     2341 ggcggcaata gtttccacca gtgggcgaaa ttccgcgccc cacgggcgag tgacagcaat
     2401 tccggcacca gtgccaatga tcaccgcaaa caaactcgaa attcccacca gccagaaatg
     2461 agccagtgcc agagctgcaa aactttcttg ctgataaacg ggtcgtggca gttgtgggaa
     2521 caaggcagca aacagcggct ggctgtaagg cagccagaaa atcagtgcca caaacaaagc
     2581 aatgagccag aacagcggat cgcgcaacat cttcatacgc ttacgcctcc tccagcagat
     2641 cctgaaaatg cagcgtgccg caaggctggc cctgcgtgtt caccaccggc agcacctcgc
     2701 atccccgcgc gacaaacagg gagagcgcat cgcgtagcgt catctcttct gccagtgcct
     2761 caccttctgc ccgttcttcg cgacgcacgt aatccgccac actacgtaac gaaagcaggc
     2821 gcacacccag ttcgctgcgc ccaaaaaact ggcggacaaa atcgtttgcc ggacgagtca
     2881 gcatcgtcag cggattgccc tgctgcacca cttcaccgtg atccatcaat accagatgtt
     2941 ctgccagccg tagcgcctca tcaatatcat gagtgaccag cacaatggta cgccccagca
     3001 aacggtgaat gcgcgtcatc tcttgttgca acgcgccgcg cgttaccggg tccagtgcgc
     3061 caaaaggttc atccatcagt aagacttgcg gatcggcagc cagtgcgcgc gccactccca
     3121 cacgttgctg ctgaccaccg gaaagctgat gcggataacg ctcacgtaaa tttgactcca
     3181 gccccagtag cgccattaat tcgtcgatac gatcgtcaat cctcgcccgc gaccattttt
     3241 gtaattgcgg cacggtagcg atgttttgcg ccacgctcca gtgggggaac aggccaatag
     3301 attgaatggc atagcccatc cggcggcgca actccagtac tggcagcgag cgaatttctt
     3361 ctccggcaaa gcggatcact ccgctgtcat gctccaccag gcggttaatc attttcaggg
     3421 tggtggattt gccggagcca gatgtgccaa tcagcaccga aaaactccct tcctgaaaat
     3481 tgagattgag atcgttaacg gctttttgtg cgccgaacag tttgctgaca tggccaaatt
     3541 caatcattac gtttcacctt cagcagtgcg ataagcaaat cgaacagcgc gtcggtaagc
     3601 accgccagaa caattaccgg gatcaccccc agcaacacta aatcaatggc gctgcttagc
     3661 agcccctgga aaaccagcgc accaaaaccg cctgcgccga ttaacgccgc aatcaccgcc
     3721 atacctacag tttgcaccat caccacccgc aggctgcgca gaaataccgg taacgccagt
     3781 ggtaactgaa catgcaggaa tcgctgcgcc ccgctcatgc ccatcgctct ggcgctctcc
     3841 agcacatcgc gcgggatctg gttcaaaccg actaccacgc cgcgcaccag cggcagcaag
     3901 gcatagagca ccagcgcaat cagtgcgggt gtcattccgg ttcctgctat gccgagcgtc
     3961 cccagccacg gaaaggccgt aaccagcgcg gcaagcggcg caatcaacag gccaaagagc
     4021 gccaccgaag gcacggtctg aatgacattg agcagagaaa aaattgcccc ctgccgagca
     4081 gtggaaaagt agcaccagat gcccaacggc acaccaatca ctaacgcagg cagcaccgca
     4141 ccaaacagca acgtcagatg ttgtgccagc gcgtcgtcaa acacatcctg acggttggcg
     4201 tattctttca ttagtgagag atcgttaagc gtgccggagt acagcaacca cagcggaata
     4261 atggcaatct gcatatgcaa caaccagcgc cacagcggat gcgtggagat tcggcggatg
     4321 gcatcactac aggccagcaa tgccagcgcc gcggccagcc agaaaccact gccgaggctg
     4381 gtacgcgcca gcgcactgcc attttgcgcc agttgggtcg ccgcctttcc tgctccccac
     4441 accagcaata cgaagacgaa ttgcgccaga atgagtgcaa aaatgctgcc ttttttagcg
     4501 ggaataaaac aggctgtcag ccaggcgcaa ccaacgccca ccagcatcca gatcgtttgc
     4561 ggccacagtt gccagagatg acgcccttca cccgagacta aacgattagg cgcgtaactg
     4621 ataaacggca gcgccgctgc gattgccgtc agcaacagca gcagcgccag aacaggatta
     4681 atacgtagat aagtcacggg aaattacttc gtccaccctt tttgtttcag gtagtcggca
     4741 gccacttttt tggcatccag tccttccact gcaatgctgg cattcagttg ctgcaatgtt
     4801 tttgcatcga ggctggcgaa gactggctgt agccactgtg ccatttgcgg atactctttc
     4861 agcaccgact cacgcaccac tggtgcaggc gcgtagatag gttgcacgcc tttcggatcg
     4921 cttaaggttt gcagccccag cgccgcgacc ggaccgtcag tgccgtaagc cattgcggca
     4981 ttaacgccag aagtttgctg ggcagcggct ttaatcgtta ccgccgtgtc gccgccagcc
     5041 agtgacagta actgatcctg atcgagttta aagtcgtagg ctttttcaaa cgcgggtaac
     5101 gcatcggcgc gttcgataaa ctctgccgag gctgccagtt tgaaggtgcc gccctctttc
     5161 agataacgac tcaggtcggc aagcgaagtg agtttgtttt tctctgccac atcctgacgc
     5221 acggcgatgg tccaggtgtt attcgctggt gcgggcgtca gccagattaa cttgttttgc
     5281 tctgcatcga gttttttgac tttctcgtaa ccttgctgcg cgtttttcca tgccgcatcg
     5341 ttttcatctt taaagaagaa agcgccatta ccggtatatt ccggatagat atccagttca
     5401 ccggaagtaa tcgccccgcg caccacagga gtcgttccga gttgcacttt attcaccgtc
     5461 ggtacgccgt ggctttccag cacctgcaag ataatgttgc cgagtagcgc gccttcggta
     5521 tcgatttttg aaccgacttt aacgggggaa gccgcctgta gcggcaggct cacggctgcc
     5581 aacataacca gtgaacctgc ccagaccttt gagagtggca tgatgctttc ctcattcttt
     5641 tactgttgtt ttcagcgaat taagagaaaa gcatagttga taatgacaga gttagcctga
     5701 gcggaaggat tcagttgcag aatcagataa atacttaaag ggcatatttt gcccggcgtt
     5761 tcttgccgga tgcggcgtaa acgccttatc cggcctacaa aggtcgaaaa ttcagcaagt
     5821 taagatatga acatcaggca aagggcgcaa acgtcgcgcc cttactaaag cattacagca
     5881 actcaaactc gcctttctta acgcgtgcgg aatcagtgcc gataaagaca ttgaacttgc
     5941 caggttcggc gtcatatttc atctgttgat tccagaactt cagcgcctcg atatcaatcg
     6001 ggaagctgac agtctgagtt tcgcccggtt tcagggtgat tttctcaaag cctttcagct
     6061 gcttcactgg acggctcatg gaagccgtca catcctgcaa gtacatctgt actaccgtgg
     6121 ccccttcgcg tttaccggtg ttcgtcacct gcacgctggc ggtcactttg ccgtcacgct
     6181 tcatggtcgg cgcagaaagt ttcacatcag agacggtgaa agtggtatag ctcagaccat
     6241 agccgaacgg ataaagcgcc ccgttagctt catcaaaata acgcgaagtg tatttgttcg
     6301 gcttgtcggc attatacgga cgaccggtat tcagatggct gtagtacacc gggatctgcc
     6361 cgacagaacg cgggaaggac attggcagct tgccggacgg gttgtaatcg ccaaacaata
     6421 catcggcaat tgcattaccg ccttcagtcc ccgcaaacca ggtttccaga atcgcatcag
     6481 cctgctgatc ttctttcacc agcgccagcg gacgcccgtt catcagcacc agcaccagcg
     6541 gtttaccggt ggctttcagc gccgcaatca agtcacgttg gctttgcgga atagtgatat
     6601 cggtacggct ggaggcctcg tgcgccatcc cctgtgcttc accgaccaca gccaccacca
     6661 catcagattg tttcgccgtc tgcaccgctt catcaatcat ctcttgcggc gagcgcggat
     6721 cgactttgac cgcttcttca tactgattca ggaaatcgat aatgcctttg tcactggtaa
     6781 cgttcgcccc tttggcatac agcactttac cgttttcacc gacggcattt ttaatcccgg
     6841 tcagtacggt cacggattga tcggcaacac cggctgcgga ccagctgccc atcacgtcac
     6901 gtttactgtc cgccagtggc ccaaccaccg caatggtggc cgattttttc agcggtaacg
     6961 tttcgagacg gtttttcagc aacaccaggc tttcgcgcgc cacttcacgc gcttctttac
     7021 ggtgcaggcg gctttcggca ttggtatcca ccgggtcaga ctctttcggc cccaaatggc
     7081 tgtatgggtc gttaaacaat cccatatcat atttaacgtt cagtacatgg cgggcagcat
     7141 cgtccagctc ttccatcgtc actttgccgg atttgatcaa cccaggcaga tacttcgagt
     7201 aatactcgtc gctcatactc atgttgattc cggatttcag cgccacgcgc accgcatctt
     7261 ccgggtctgc cgccgtgcca tgtttaatca gctctttgat tgcaccgtga tcggaaacgg
     7321 tgatgccttt aaagccccac tggtcgcgca gaacatcttt cagcagccag gaatcggagg
     7381 tggctggcgt gccgttcagc gagttcagcg ccaccatcac cgcgccgctg cctgcgtcca
     7441 gccccgcttt gtacggcggc atataatcat taaacaggcg ctgcggactc atatcgacgg
     7501 tgttgtactc tttaccgcct tctaccgcgc cgtatgcggc aaagtgtttg acgctggtca
     7561 tcaccgagta gcgatctgcc gggcttttac cctgcatcgc ttccaccatg gttttaccca
     7621 ttgttgaggt gagatacgta tcttcgccaa aaccttcgga agcacgtccc cagcgcggat
     7681 cgcgcgagac atcgaccatc ggtgcccagg tcatattcag gccatcatct gccgcttcat
     7741 aagcagagac acgtccgacc gttttcactg catcgaggtt aaaagacgag gccagaccga
     7801 ggctaatcgg gaacaccgtg cgctgaccgt gcagcacgtc gtaagcaaag aaaagaggaa
     7861 ttttcaggcg gcttaattcc atcacctgat cctgcatggc gcggatatcc tgacgggtta
     7921 cggtgttgaa aatcgcccca acctgaccgt ctttgatcat ctcgcggatc gcctctttcg
     7981 ggttatccgg accgacgctg attaagcgca gctgaccaat tttctcatca actgtcattt
     8041 tcttaagcag ttcggtgacg aacgcatccc gcgcttcggg cgttaatgga tggttgccga
     8101 ataaatcatc cgccagtgct ggctgcaggg ccagactcac cgcgattcct actgaacata
     8161 gccatttcat atggattttt cttcctctgt tgcccgacga ggcagcgaaa atggtgcaaa
     8221 aaccgtagtt tgccataagc atgatggaga gagaaaaaga atgctcagtt tattgtctga
     8281 attttcaaaa tattcactcg ctgaattgtc atacaaggcg ctattctagt ttgtgatatt
     8341 ttttcgccac cacaaggagt ggaaaatgtc ttccatgaca acaactgata ataaagcctt
     8401 tttgaatgaa cttgcccgtc tggtcggtca ttcacacctg ctcaccgatc ccgcaaaaac
     8461 ggcccgctat cgcaagggct tccgttctgg tcagggcgac gcgctggctg tcgttttccc
     8521 tggctcacta ctggaattgt ggcgggtgct gaaagcctgt gtcaccgccg acaaaattat
     8581 tctgatgcag gctgccaata caggcctgac cgaaggatcg acgccaaacg gtaacgacta
     8641 tgatcgcgat atcgtgatca tcagcaccct gcgtctcgac aagctgcacg ttcttggcaa
     8701 gggcgaacag gtgctggcct atccgggcac cacgctctat tcgctggaaa aagccctcaa
     8761 accgctggga cgcgaaccgc actcagtgat tggatcatcg tgtataggcg catcggtcat
     8821 cggcggtatt tgtaacaact ccggcggctc gctggtgcaa cgtggcccgg cgtataccga
     8881 aatgtcgtta ttcgcgcgta taaatgaaga cggcaaactg acgctggtga accatctggg
     8941 gattgatctg ggcgaaacgc cggagcagat ccttagcaag ctggatgatg atcgcatcaa
     9001 agatgacgat gtgcgtcacg atggtcgtca cgcccacgat tatgactatg tccaccgcgt
     9061 tcgtgatatt gaagccgaca cgcccgcacg ttataacgcc gatcctgatc ggttatttga
     9121 atcttctggt tgcgccggga agctggcggt ctttgctgta cgtctagata ccttcgaagc
     9181 ggaaaaaaat cagcaggtgt tttatatcgg caccaaccag ccggaagtgc tgaccgaaat
     9241 ccgccgtcat attctggcta acttcgaaaa tctgccggtt gccggggaat atatacaccg
     9301 ggatatctac gatattgcgg aaaaatacgg caaagacacc ttcctgatga ttgataagtt
     9361 aggcaccgac aagatgccgt tcttctttaa tctcaaggga cgcaccgatg cgatgctgga
     9421 gaaagtgaaa ttcttccgtc cgcattttac tgaccgtgcg atgcaaaaat tcggtcacct
     9481 gttccccagc catttaccgc cgcgcatgaa aaactggcgc gataaatacg agcatcatct
     9541 gctgttaaaa atggcgggcg atggcgtggg cgaagccaaa tcgtggctgg tggattattt
     9601 caaacaggcc gaaggcgatt tctttgtctg tacgccggag gaaggcagca aagcgttttt
     9661 acaccgtttc gccgctgcgg gcgcagcaat tcgttatcag gcggtgcatt ccgatgaagt
     9721 cgaagacatt ctggcgttgg atatcgctct gcggcgtaac gacaccgagt ggtatgagca
     9781 tttaccgccg gagatcggca gccagctggt gcacaagctc tattacggcc attttatgtg
     9841 ctatgtcttc catcaggatt acatagtgaa aaaaggcgtg gatgtgcatg cgttgaaaga
     9901 acagatgctg gaactgctac agcagcgtgg cgcgcaatac cctgccgagc ataacgtcgg
     9961 tcatttgtat aaagcaccgg agacgttgca gaagttttat cgcgagaacg atccgaccaa
    10021 cagtatgaat ccggggatcg gtaaaaccag taagcggaaa aactggcagg aagtggagta
    10081 aaaattacgg atggcatagt atcgccatcc gtcattcact taatcgttct gtgccgtctg
    10141 ccccgccgcc gccatttggg cggctttttg ttttttatag ctcaacgctg ctgctggaac
    10201 aggcatcact ttaccggttt caatccaggt acgcaggcgg ctggcatcgg caaaatgggt
    10261 atatttgcca aacgcatcca tcactaccag cgccaccggt ttattattga taaccgtacg
    10321 catcaccaga caatggcccg ccgcattggt aaaaccggtt ttggttaact gaatattcca
    10381 gttatcgcga tacaccagat ggttagtatt gcggaacggc agcgtatacg tcgggttaga
    10441 gaaggttgcc atatcttccc gggtagtact taactgcccg atcaacggat attgtttgct
    10501 ggcaatgagc agtttggtta agtcacgggc agttgaaacg ttatgcaccg acaatccggt
    10561 aggttcaaca aagcgcgtgt tgttcattcc gagcgatttc gctttcgcat tcattgcctt
    10621 aataaaggct ttgtaaccac cgggataatg gtgtgcaagg cttgccgccg cgcggttttc
    10681 tgaagacatc agcgccagca acagcatatc tttacggctg atttcgctat tcagtcgtac
    10741 gcgcgaatag acccctttca tctccggcgt ctggctgata tccactttta gtttttcatc
    10801 cagcggcagt cgtgcatcca gcacaaccat cgcggtcatt aatttgctga tagacgcaat
    10861 cggacgcacc agatccgggt ggttcgagta gatcactttg ttggtattca gatcaacaat
    10921 catcgcgcta ccggaggcaa tttcaggttg tgaagcggtg gtagcggctg ccgttttcgc
    10981 aacggcctgc ggtgccaaag gcacagccag catcagggcc aggctaaata aagaaactcg
    11041 aaatttcggc atgatgagca ttcagatagt ggttcacgcg cacaggttgc gcaccgccgg
    11101 agtaaggatt tactgaggct agcgacgcca tcataacgag caaaaagtgc gatcgtcaaa
    11161 ggagaatcgt gaggaaatgc tgcattgctg acatttacgc cagcaatgca acgtcaaaga
    11221 actttctaga acaaacgata accgtagccc catagtataa cggttagggc gagcagcact
    11281 tccagtacca gcacgccaat cgccagcgtc gaactagaga agctaaggcc ttcctctttg
    11341 ttgatattca ggaagctcgg aataccaagg taaagcaggt agccggtgta aaacagcgcc
    11401 accgtgccga ccagcgcaca caaccagacc agtggataaa gcgccaccag accgcttaaa
    11461 aacagcggag ttgcaacgta gcccgcgaag accatgcagt gcgcaagtga cggacgctgc
    11521 ggataattac gcgccatcca ccagatgacc cgccccatta ccgcaacccc agccagcata
    11581 acgccataaa acaggacagc cagcgccagt ccggtaaacc aggataactt caggatagtg
    11641 ccatcgccaa aattccagcc aatctgtgta gtgccaatga aggcgcaaat caccggaatc
    11701 gccgccatca gcaaaacgtg gtgggtgtaa tggtgagaaa tcgtttcgtt ttcgcgatta
    11761 atcacctgca tttcacgatc gggatgggaa aacagtcccc agacatggct cataccgccc
    11821 ccttgttgtg agttcatgaa cctgacagtt caagtataag tcagcttgtg attatttttt
    11881 gttcgctcat gtaatttgca ccgtctgatg tacgtccttt ttcagcgtga atgatttcca
    11941 gggtgtatgc ttacaggcaa ccaaagggag acagactggc ctatggatct caatacactc
    12001 atctcacaat atggttatgc cgcgctggtg atcggtagcc tggcggaagg tgaaaccgtg
    12061 actttgctgg gaggcgttgc ggcacatcag gggctattaa agttcccgct ggtggtactt
    12121 tctgtggcgc ttggcggcat gattggcgac caggtgcttt atctgtgcgg gcggcggttt
    12181 ggcggcaagc tgttacgccg tttctcgaaa catcaggata aaattgagcg ggcgcagaaa
    12241 cttatccaac gccatccgta tctgtttgtc attggtacgc gctttatgta tggctttcgg
    12301 gtgattggcc cgacgctgat tggtgccagc cagctgccgc cgaaaatctt tctgccgctg
    12361 aatattctcg gcgcatttgc ctgggcgttg atttttacca ctattggtta cgctggtggt
    12421 caggtgattg cgccgtggtt gcacaatctc gaccagcatt tgaagcactg ggtctggttg
    12481 attctggtcg tggttctggt ggtgggcgtg cgctggtggc tgaaacggcg cgggaagaaa
    12541 aagccggatc atcaggcgta aaaccattgc cctgggtaag gcgttcgcgc cgcatccgac
    12601 atccggtgca ccatgcctga tgcgacgctt gtgcgtctta tcatgcctac caaaatttta
    12661 cccattgaaa taaccacgca cctcactgcg ctattctggg ttgaactgcg gattcgccaa
    12721 cataaagccg ccatccacta tcaacgactg cccggtggtg taatttgcgc cctctgagca
    12781 cagccacgct accaggctgg caatctcatg cgttgcgcca aaacgccgca gaggaatcga
    12841 aggctccgcg tcgggcttca cgtcgctgtc atccatgcca ttcattggcg tggcgatcgc
    12901 cccaggcgcg actgcgttca ccaaaatctt atgcctgacc agctccagcg ccatcgtttt
    12961 ggttaacccg ccgagcgcat gtttagcggc tgtgtaagcg ctggcatccg gcagcggcgt
    13021 atgttcatgt accgacgtaa tgttgatgat acgaccgccc tgcccttgtt tcaccatctg
    13081 gcgggccgca atttgtgagc ataaaaatgc accatcgaca tcaacggtaa aaatcttgcg
    13141 ccactcatcg aaagccatat caagaaacgg cgctttggtc attgcgcccg cattattcac
    13201 cagcacatca atacgcccca gccgttgaat gagtttctcc agcgcctgcg caccttctgg
    13261 cagtttaccg agctccagct gcacgatctc cgcacgtacg ccgtggctaa ctacctcacg
    13321 cgcggtatct tttgcccctt cttcatctga gtgccaggta atgccaatat caaacccctg
    13381 ctgcgccagt aataacgcgc actctttgcc gatccccgaa tcggaggcgg taataatcgc
    13441 aacctgtgcc atcaagttct ccacttaacg ctgaataaac gttaagtata gaaggcgcat
    13501 atcatcagcg tttgtaaccc ccgcccaacg caccagtgag ttgaatagaa gcatccagcc
    13561 actgcccttg caataacagg ccattggccc gctcacgcag cgcggggatt ctggcttcgc
    13621 tgacgcggga accagcaatg atgcccgcgt taaagcgcgc ctgcgcaaga cctaccacac
    13681 gcaaggcatc gcgctcaatt tgcgcctgat gctggttttt ctccgcaagt gtctgaacct
    13741 gactggctgc ccgcgccacg tcattcaccg cttcaaccac cgctttgttg tagctggcga
    13801 tagacaagtt gctttcggct tttgcgatat tgagattggc gttaagacga ccactatcga
    13861 aaatgggtag cgtcaggcct gccgtaacgc ccatttgctg cgcggaatga cggaacagat
    13921 cgcttaagtg caacgcatcc tgttgcagga aggccatcag gttgatgtca ggataaaatg
    13981 ccgcttttgc cgcatcaatg gtgcttagcg atgactcaac gtaccagtgc gccgcctgca
    14041 aatctgcccg ccgggccagt aaggagtacc ccagttcatc aggaagctgg cttgccactt
    14101 tcggcaacgc gaccggttta agcttcaatg actttgtctg gttatttgta agtgcgctta
    14161 accgtgcctc aataattttc attttccccg cgacatcgtt gagctgctgc cgggttttgc
    14221 tggcattaat atcggtttcc acaccttcaa ctgaagaagt aatcccgttc tgatatagct
    14281 ggcgatcagt cgcgataatg gtgttctgct ctttttctat ttgctgcaag accgtgttta
    14341 acgccgcctg ggtttgccac tcccagtaca ggcgggctac gctgccagcc agcaattggc
    14401 gggtttgctc gcgttccgcc gcccgtgctt taaccgtacc caggcgggca gtaacctccg
    14461 cccgattctt tccccagata tcgagatgcc agcccgccgt taagccaaaa gtaccgttgg
    14521 tgtaccacgg gccggtcgta cctgcggccg gatcgttcag agcaaacggc cccattaagc
    14581 cttctgccga cattttttgc cgctccatat ccgccgaaaa gtcgatctgc ggaccatcct
    14641 gagtggcaac tgccttcgcc tgggcttcag ctagctgaat gcgctgttca gccacctgca
    14701 tatccggtgc gttctgtagt gcattgttaa ttaatgaagt gagctgatta tcgtgatact
    14761 ccagccacca ttggctgtct ggccaaccat ttttcagcgc cgtgggtaat gcggtgtcaa
    14821 cttgtgcagc gggcgtttgc tggcttaacg cctggcgggt ttcatgcata ggcgcacacc
    14881 cggccagcat cagtaacagc ggaaaacagg cgatggctgg ataaaaggaa tcacgattca
    14941 tgggggaata atcaggtaag aaacggtgcg cggagattac cgtgtgttgc gatatatttt
    15001 ttagtttcgc gtggcaatac atcagtagca ataaaacgac atacccagaa aaatatagac
    15061 taagtaaatg atatcttccg atttatctta atcgtttatg gataacggca aagggcttcg
    15121 ttttttccta tacttattca gcactcacaa ataaaggaac gccaatgaaa attatactct
    15181 gggctgtatt gattattttc ctgattgggc tactggtggt gactggcgta tttaagatga
    15241 tattttaaaa ttaattaatg tcatcaggtc cgaaaataac gagaatattt cagtctctca
    15301 tcctgttgcg ctcctgtcat gtgcattgct tcatataatc actggcgca
//
LOCUS       DAZPDU010000102        15177 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZPDU010000102 DAZPDU010000000
VERSION     DAZPDU010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15177
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.103"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(17..2002)
                     /gene="yjcS"
                     /locus_tag="ACHURS_002818"
     CDS             complement(17..2002)
                     /gene="yjcS"
                     /locus_tag="ACHURS_002818"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFR1238499.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTIGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            2255..2572
                     /locus_tag="ACHURS_002819"
     CDS             2255..2572
                     /locus_tag="ACHURS_002819"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HFR1238500.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            2641..2970
                     /gene="yjdP"
                     /locus_tag="ACHURS_002820"
     CDS             2641..2970
                     /gene="yjdP"
                     /locus_tag="ACHURS_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFR1238501.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(3117..3875)
                     /gene="phnP"
                     /locus_tag="ACHURS_002821"
     CDS             complement(3117..3875)
                     /gene="phnP"
                     /locus_tag="ACHURS_002821"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFR1238502.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACDRARRSPQYRRQPCSGVV
                     KFNEAITLIDAGLHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPIYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVMDCSHPPRADAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(3877..4311)
                     /gene="phnO"
                     /locus_tag="ACHURS_002822"
     CDS             complement(3877..4311)
                     /gene="phnO"
                     /locus_tag="ACHURS_002822"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFR1238503.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFSANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            complement(4298..4855)
                     /gene="phnN"
                     /locus_tag="ACHURS_002823"
     CDS             complement(4298..4855)
                     /gene="phnN"
                     /locus_tag="ACHURS_002823"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFR1238504.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            complement(4855..5990)
                     /gene="phnM"
                     /locus_tag="ACHURS_002824"
                     /pseudo
     CDS             complement(4855..5990)
                     /gene="phnM"
                     /locus_tag="ACHURS_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418519.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
     gene            complement(5987..6667)
                     /gene="phnL"
                     /locus_tag="ACHURS_002825"
     CDS             complement(5987..6667)
                     /gene="phnL"
                     /locus_tag="ACHURS_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFR1238505.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLIVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMEASS"
     gene            complement(6778..7536)
                     /gene="phnK"
                     /locus_tag="ACHURS_002826"
     CDS             complement(6778..7536)
                     /gene="phnK"
                     /locus_tag="ACHURS_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFR1238506.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            complement(7533..8378)
                     /gene="phnJ"
                     /locus_tag="ACHURS_002827"
     CDS             complement(7533..8378)
                     /gene="phnJ"
                     /locus_tag="ACHURS_002827"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFR1238507.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTECTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            complement(8371..9435)
                     /gene="phnI"
                     /locus_tag="ACHURS_002828"
     CDS             complement(8371..9435)
                     /gene="phnI"
                     /locus_tag="ACHURS_002828"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFR1238508.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLTKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            complement(9435..10019)
                     /gene="phnH"
                     /locus_tag="ACHURS_002829"
     CDS             complement(9435..10019)
                     /gene="phnH"
                     /locus_tag="ACHURS_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFR1238509.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALYQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLATPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTDTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            complement(10016..10468)
                     /gene="phnG"
                     /locus_tag="ACHURS_002830"
     CDS             complement(10016..10468)
                     /gene="phnG"
                     /locus_tag="ACHURS_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFR1238510.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            complement(10469..11194)
                     /gene="phnF"
                     /locus_tag="ACHURS_002831"
     CDS             complement(10469..11194)
                     /gene="phnF"
                     /locus_tag="ACHURS_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFR1238511.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGIGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            complement(11215..11994)
                     /gene="phnE"
                     /locus_tag="ACHURS_002832"
     CDS             complement(11215..11994)
                     /gene="phnE"
                     /locus_tag="ACHURS_002832"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFR1238512.1"
                     /translation="MQTITIAPPKRSWFSLLSWAIVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            complement(12120..13136)
                     /gene="phnD"
                     /locus_tag="ACHURS_002833"
     CDS             complement(12120..13136)
                     /gene="phnD"
                     /locus_tag="ACHURS_002833"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR1238513.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQGVKSNNGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            complement(13161..13949)
                     /gene="phnC"
                     /locus_tag="ACHURS_002834"
     CDS             complement(13161..13949)
                     /gene="phnC"
                     /locus_tag="ACHURS_002834"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFR1238514.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESAGIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            complement(14082..14525)
                     /gene="yjdN"
                     /locus_tag="ACHURS_002835"
                     /pseudo
     CDS             complement(14082..14525)
                     /gene="yjdN"
                     /locus_tag="ACHURS_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
     gene            complement(14683..15018)
                     /locus_tag="ACHURS_002836"
     CDS             complement(14683..15018)
                     /locus_tag="ACHURS_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFR1238515.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
BASE COUNT         3087 a         4188 c         4480 g         3422 t
ORIGIN      
        1 ccaaaagcga gtgacattaa tttggggtta cgatattcac ccacaaatcg aaattatcca
       61 gacaggcgat aatttcttcc agtttcgcgc cattgccaat aattttggct ttcttcgctt
      121 ttaccagatc cgccattttg gcttgcccgg tcagcacggc gtgcagatct tcacggctga
      181 tgtagaaaga ggcgtcagct tgggattgca gtgttttacg gtagttaagc acgctatcgt
      241 tcagcgtcag gttgaggtta tcgccattgc tcatattgaa gttcaggctg atatttttac
      301 ccgcggcttt cgcgctatcg aggcgaacgg acataaagtc gaacagcatt tcgaccgaca
      361 tcccgcgaat ggtgtccggg gaaccgatgg tgccgtggct gaacttatgc accccttcgc
      421 gcagctcttt cgcgccggtc aggtagaaac cgcgccaggt ggcggactcg gcctgatagc
      481 ccagctgttc aaagttattc gcttgcagat tcttcgcgac ctggtcaccc gggttggcgg
      541 caatcacctg tttcagcagt tctgccgacc agcggtaatc accttgcttg ttcgcttctt
      601 gcgccaggtt gatgacacgg gcagaaccgc ccagcgcctg cacgtaacgt ttacccatct
      661 ccacctgacc atacggatgc aggttagccg ggttaccgtc gtaatagcca agatagaagt
      721 tatacaccgc gcgggcgttg tggctgacag aaccgtaata gccacggctg gcccagttat
      781 tggcaagtgc aggcggcagt ttaatcatgt cgccgatttc attcatggtg tagccctggt
      841 tcgccaggtg cagggtctgg tcgtgaatgt acttgatagt atcgcgatat ttaccaatat
      901 aatcattgat atgcttattg ccccagaccg gccaggtgtg cggcataaac aggacttccg
      961 cgtcgttacc ccacatatcc agcgtttcgt tcagatactc ggtccacttg ctggtgtcgc
     1021 gggttttcgc gccgcgcaga gtgtagaagt tgtgcagggt atgcgtggcg ttctcggcgg
     1081 tacacagcgc tttcagggcc ggaatataga agtgcatttc ggctggtgct tcgctacctg
     1141 gggtcatcag gaagtcaaac tccaggccgt cgataatcat cttctcaccg gttttggtga
     1201 tggttttgtt cggtgcgatg atgctcgggt agcccgatgc cagcgttacg ccaagaccat
     1261 tgccgatgtt accctgaggg ttatgcgcca gcagcaggcc gtaggagtaa agtgcacggc
     1321 ggctcataat attacccgcc agtacgtttt cgctgatggc ttcgtccata aagccagcag
     1381 gggcgataac ctgcacttta ccggatttca catcagcttc agagataatg cctttcacgc
     1441 cgccatagtg gtcggtatgg ctgtgggtat aaataaccgc aacaatcggt ttttgcgggc
     1501 gattttggaa gtaaaggtca agggcggctt ttgcgctcgg tggcgtcacc agcgggtcga
     1561 tgacaataat cccggtatca ccttcgataa aggtgatgtt ggagatatcc tgaccgcgaa
     1621 cctggtacat tctgtcggtg actttaaaca ggccggaaat accgtttaac tgcgactgac
     1681 gccacaggct agggttaacg gtttcgggcg cgggcgcatt gatatcaaat ttataatctt
     1741 ccccgcgata atatggtttg ccattagcat cgcgcagaat accttcatcc agcagcggag
     1801 cgataaatcc acgctgagcg tcgtcaaaat cctgacgatc gctaaacggt aatgatttgg
     1861 cgtaattctg attaacctgt tgggtatatt gagtggcggc tttcgcttcc tctttcgcgt
     1921 tagcggtatt taccatcatg ccgctggcgg cagttaacgc aataacaatt ctgctcaaac
     1981 ggaataaccg agagttattc atgtaaatct ccatttatca tttatgtcgt aaatatgtaa
     2041 tccatgcaaa atacgtcatt taaagctggg gttatcataa ttgggaattt gcgactttaa
     2101 taagtggaag tgtgagctga acgatccatt ttattaggca tttgtgctga agagggaaaa
     2161 ttacgcgaat ttattatctc tgtaaataat atgaggtgtt ttatatattt attatatata
     2221 ttgtacttcg gcaaatactg agcacaattt atcaatgcaa ccggtgtttc tttttaaaaa
     2281 acgtaccgga gcaatgggcg gtcatgtcgc tggcaccgca ggattctgcg ttgctgtgcc
     2341 agccttttat tcatggtcaa tgagtgaggt gattgataaa gacaccaggt cgtcagagcg
     2401 cactgattat ccgcattatg ccagtgaagt tgcactggct tttggttcac gagtggttgg
     2461 cctcgaactg gcaaaaatga ttgtggatgc gtggctgggc gcacagtacg aaggcggtcg
     2521 tcatcaacaa cgcgtggagg cgattacggc aatagagcag cggagaaatt gagattcatc
     2581 cactacttgc atggatgagt aatgactaat gtggatagag tttctttttg aggttggcta
     2641 atgaaacgct ttccgctatt tcttttattc accctgctca cgctgtccac cgttccggcc
     2701 caggctgata ttatcgacga tactattggt aatattcagc aggcgattaa tgacgcttat
     2761 aaccctgacc gtgggcgaga ctatgaagat tcgcgcgacg acggctggca gcgtgaagtg
     2821 agcgacgatc ggcgcagaca atatgacgac cgccgccgcc agtttgaaga tcgccgcagg
     2881 cagctggacg atcgccagcg ccagcttgat caggagcgtc ggcaactgga ggatgaagag
     2941 cggagaatgg aagatgagta tgggcgatga ggttgggtat ggagcaggca tcgcccctca
     3001 ccccaatggg gagagggaga aaacgagcgc aatattcaat atcgagcaca atcggtcccc
     3061 tcgcccctct ggggagaggg ttagggtgag ggggccagcg cgcacattcc ctctcatcac
     3121 gccaccccaa tctccatccc atcaaacccc acctcaaacc ctgacggtag tgcgttttcc
     3181 atcagccacg catcaaactg gtggctgata tgggtcagaa tcacccgtgg cgagcggata
     3241 acctgattca gcgcaagcac ggtatttaaa tcacagtgat tacgcggtgc atccgcgcgc
     3301 ggcgggtgac tgcaatccat caccattacc tgcggctgat tattgcgtaa aaatttcagc
     3361 gttttttccg gcaagccagc ggtgtcagac agccacgcca cccggctgtg cgccgtttcc
     3421 agtaaatagc cgaaagtgag ttttgagtgg ttaagcggca gcggcgtgac ctgcaacccc
     3481 tgcaaatcaa acaccacaaa cggttccacc gtgtggctga aatcaagcag cccgggatgt
     3541 ttaaacagat cgtcgcagcc ctgttcatcc ggcgggccgt agatcgggat cggatcgccc
     3601 acgccccagc gcagcggaaa cagcccctgg acgtgatcca tatgataatg cgtcagcaaa
     3661 aactgctgga acgatccggg agaccagcga tcggtgagat cgtgcagccc ggcgtcgatc
     3721 agggtgatcg cctcgttaaa cttcactacg ccgctgcacg gttggcggcg atactgcggc
     3781 gagcgccgcg ctcggtcgca ggccgcgcac tcgcagcccc atgccggaac gccctgtgcg
     3841 ccgccggtgc cggtgagcgt gagggtcaga ctcatgttac agcgccttgg tgaagcggaa
     3901 gtgactctgc tcgtagcctt ctcgcagata gaaacggtgc gcgtcgtggc gcttcacgtt
     3961 ggtcgaaagc tcggtcattt cggccccggc ctggcgggct tcttcttctg cccacgccag
     4021 caacttgcta ccgacgttca ggccgcgcgc ctgcggcatc actaccagtt cctgaatttc
     4081 gccgatccag ttgacatgat gcagatgaaa ctgcaaatgc aggccgatca tgccgacaac
     4141 ctcgccatcc agcagcgcca gatgatagcg catgttcggg tcgcgcagat tggcgctaaa
     4201 gcccacgcga aatgcgtggt ggtcaaactc cgcctgtttt agctcacaaa tcagcgcgta
     4261 aaccgcgtcg gtatcatact gcgtggccgg gcgaagctca caagcaggca tggtgtttct
     4321 ccttctgatg aatcagcgtc agcagcgtgt cgaccgactg gcgcaggctg ccgtcattgt
     4381 tgagcgtatg gcaatcctgt ggagtatagc gggcggcgcg cgccaggcgg gcgttaattt
     4441 cactggcgtt ttcacggcca cggttttcca ggcgctggcg gaggatctcc ggcgaaacct
     4501 gtaaacagac gggcagcagc gccgattgat agcgcgcccg cgcctgcggc agatgggcgc
     4561 gtgagccgtt gaccagcacg tcgaatccgg cgtgcagcca gaggtcgatc tcgacgccga
     4621 cgccataata cagaccgtta gcgtgccagc ttaaggccag taggttctgc cccgcgcggg
     4681 taaaaaactc ctgctcgctc agagcgatat ggttttcgct tccggcgctg gcgtcgcgcg
     4741 tgatgtagcg atgcgccacc agtagctgag tttgttcccg caggcggagt tccgccagca
     4801 ggctatcttt cccggagccg gacggcccca ttaaccaaat cagttttccc atcatcagaa
     4861 caccctttta ccctgacgcc agacgtggtc gatatgaatg tgattgccct tgcgatgcgc
     4921 cagcaccaag tccgcgcgtt tgccctcgcc aatcaccccg cgatcctgga gattaagcgc
     4981 ctgcgctgga tttttagtca ccagcttcac cgcctgcggc agcgtaaagc ggttgctctc
     5041 gtcatcggcg acgcgaaatg ccgcatcgag caggctggcg gggtagtagt cggaagagag
     5101 gatatccagc aggccaagct gcgccagttc actggctgcc acgttgccgg agtgcgagcc
     5161 gccgcgcaca atattcggcg cgcccatcag cacgttcatg ccatgcttgc gcgaggcttc
     5221 cgccgcttcg aacgtggtgg gaaattcggc gatcacgctg ccaagctggt gagattcagc
     5281 aacgtgggcg tgggtgcgtc atcgtggctg gcaagcgcaa tttttcgcgc gcggcacagg
     5341 gcggcgatgg attcgcgatt cggctgcgac cagcgtgcgg cgagcgccag ttgctcttct
     5401 tcgtactgct gcatctgcgc atcagtgagg gagtatttgc cctgataata ttcgcgatac
     5461 ttctcgcgat tggcgaactg gcgctggccc ggcgagtggt ccatcagcga caccagcgtc
     5521 accggctcgc gctgcaccag tttttcaaac agcggcagcg tggtgtgatg cggcagttcg
     5581 cagcgcagat gcagacggtg ctcggcgcgg ttgacgccgc gtttctgcgt ctcttcgatg
     5641 gcgttgatca tcttctccag attcaccagc cgatcgccgc cgtcgcgcac gtcgccaatt
     5701 gccacggcat ccagtacggt ggtgatgccg ctcgccacca tcagcgcgtc gtggctgctc
     5761 atcgccgagt gggcaggcca gtcaactttc gggcgcgggg tgaagaattt atccagatta
     5821 tcggtatgca gctcaatcag ccccggcagc agccagccgc cttcgccgtc catcgcctcc
     5881 ggcaggcggc tctggctttc ggcaaaggcg cggatttcgc cgtcctgcac ctccagtgaa
     5941 ccgtgtacca cttcgttttc cagcaccaat ttgacgttat tgataatcat gaagaggctt
     6001 ccattgggtg caggcggtcg gcaacgtcat tacgtacggc ttcgtcgtgg aagatgccga
     6061 cgatggccgc gccacgggct ttggcttcgt gaatcagctc gactacggcg gcgctgtttt
     6121 tggcgtccag cgaggcggta ggttcgtcga gcagcagaat ggggtagtcg acgataaagc
     6181 cgcgggcgat gttgacgcgc tgctgttcgc cgccggagaa ggtcgatggc gccagatgcc
     6241 acaggcgttc cggcacgttc aggcgggtga gcagacgcgc ggctttagcg gcgcaggctt
     6301 cacgcggtac gccggtatcg agtagcggct gcatcaccac ttccagcgcc gaaatacgcg
     6361 ggatgacgcg cagaaactgg ctcacccagc caacggtggt tttgcggatc tccaccactt
     6421 tgcgcgctgg cgcggtgacc agatcgaccc attcgtcacc gtgtttgatc tggatttgcc
     6481 cttcgtcggg cagatagttg gcgtacagcg agcgtagcag ggttgatttt ccgctgccgg
     6541 aatggccgtg gagcaccacg cattcgcccg cgttgacgat gagcgaggcg cggttgagga
     6601 cgggcaggcg cacgccgttt tgctggtgca ggatgaaggt tttactgacg ttttgtacgt
     6661 taatcatttt tcgcctcgtg gttcgggtgt cggatgcgac gctgacgcgt cttatccgac
     6721 ctacggggag cgcatttgta ggccggataa ggcgtttacg ccgcatccgg caccggctca
     6781 attctgcaaa accgatgaca ccagcagctg tgtatacgga tgatgcgggt cgtcgagcac
     6841 gcggtcggtt aacccactct ccaccacttg cccctgcttc atcaccagca aacggtccgc
     6901 cagcaggcgg gcgacgccta aatcgtgggt gacaatcact accgcgaggt tcagctccac
     6961 caccagtccg cgcagcaggt cgagcaggcg ggcctgcacc gacacatcca gcccgccggt
     7021 cggttcatcc ataaacacca gcttcggatg cgtcaccagg ttgcgggcaa tctgcaaacg
     7081 ctgctgcata ccgccggaaa aggtggtcgg caggtcgtcg atacggttgg cgggaatctc
     7141 cacctcttcc agccacttct gcgcggtagc gcgaatatcg ccgtaatgtc gcgccccggt
     7201 cgccatcagc cgctcgccga tattgccgcc tgccgacacc tggcggcgca ggccgtcgag
     7261 cggatgctga tgcaccacgc cccattcggt gcgcagcagg cgacggcggt cggcctcgct
     7321 cattgcatag agggaacggt tttcatagtg aatctccccc tgctgcggcg tcagacgcgc
     7381 ggagatcgac ttcagcagcg tggtcttccc ggagccggat tccccgacaa tgcccagcac
     7441 ttcccccggc cataaatcaa aagagacatc gctaaagcct ttgcccggcg cgtaaaggtg
     7501 ggtcaggtta ttgaccgaaa gtaacggttg attcattggc ttttcgcctc gctctgttgg
     7561 cggcaataat cggtatcgga gcagacaaac atgcggtttc cggcgtcatc cagcaccact
     7621 tcatcaagat agctgtgggt cgatccgcag atggcgcatg gctcatccca ctgctgaacg
     7681 gtgaacgggt gatcgtcgaa atcgagactt tccacgcggg taaacggcgg caccgcatag
     7741 atgcgcttct cgcgccctgc gccgaacagc tgcagggcgg gcatcatgtc cattttcggg
     7801 ttatcgaatt tcgggatcgg cgacgggtcc atcacgtagc gcccgttcac cttcaccgga
     7861 taagcatagg tggtggcgat atgaccgaag cgggcgatat cttcatacag tttcacctgc
     7921 atcacgccgt actcctccag cgcgtgcata gtgcgggtct ccgtttcgcg cggctcgata
     7981 aagcgcagcg gttccgggat tggcacctgg aagataatga tctgatcttc ggtcagcggc
     8041 gtttcgggga tgcggtgacg cgtctggata agcgtcgcat cgtccgtaca ttcagtggtg
     8101 tttaccccgg tcacgcgctt aaagaagttg cgaatcgaca cggcgttggt ggtgtcatcc
     8161 gcaccctggt caatcacctt caacacgtcg ctttcgccaa tcacgctggc ggtgagctgg
     8221 ataccaccag ttccccagcc gtacggcatc ggcatctcgc gcccgccaaa cggtacctga
     8281 taaccgggga tcgccaccgc ttttaagatg gcgcggcgga tcatgcgttt ggtctgctcg
     8341 tcgaggtagg caaagttgta gccgctcaga ttagccatgg ttctgctcct gttgcagacg
     8401 tttgagtagc tccagttcgg cctggaaatc gacgtagtgc gggagtttga ggtgcgagac
     8461 aaagcctgcg gcttcgacgt tgtcggcatg cgccagcacg aactcttcat cctgcgccgg
     8521 gcctgtcgcg tgctcgccgt attccggggc ttgcaacgca cggtcaacca gcgccattgc
     8581 catcgctttg cgctcgctca tgccgaatac cagcccgtag ccgcgcgtga agtgcggcgg
     8641 ctcatccggc gggtcgataa aaccgttgac catttcacac tcggtcatca gcagttcgcc
     8701 gacgtttacc gcaaatccca gctcttccgg cacaatcgac acgtcgatgt aaccactgcg
     8761 gatctcgccc gcgaacgggt ggttgcgccc gtaaccgcgc tgggtggagt aagccagcgc
     8821 cagcaaatag ccttcgtcgc cgcgcattaa ctgctgcaaa cgggaagaac gtgaacaggg
     8881 gtaaaccggc ggcgtgcggg tgatgtcatc cggctgtgct ccgctatcct cttcaaactt
     8941 cgccagcccc tgacgcgcca gcaggctgaa aacgtgcggc gacggctgtt gttcgctgtc
     9001 ggcggtggtc agcgtcggcg cttcgccgtt tgccagcaga gtgaaatcga gcaggcgatg
     9061 ggtgtagtcg taggttgggc caagcagctg gccgccggga atatctttat aaaccgctga
     9121 aatacgccgt tcgagacgca tctccgtggt gtcgagcggc tcgcttaccg ccagtttcgt
     9181 cagcgtggtg cggtaggcgc gtagcaggaa aatcgcttcg acgttatcgc cgctggcctg
     9241 tttcagcgcc agcgccgcca gttcgcggtc ggcaatgccg ccttctgtca tcacgcgatc
     9301 caccgcgagg ttaagctgct gttcaatctg ggcgacgctc agttcgggca aatcggtatc
     9361 gccccggcgt cggctctctt gcagggcgtg ggcggcgtcg atcgcctttt cgcccccttt
     9421 cacggcaacg tacatcagca cacctccaca tgagtggttc gcgggatagc cagcaggcgc
     9481 tcgccgcagg tcaggattag gtcgatgccg agcgggaacg ggtgcgggcg ctcggtgagt
     9541 tcgtgcagaa tgcactctgg cagctgcgga gcgatcattc gttcttcggc aatgcccgcg
     9601 ccggtgagac gcaacatgcg tccgccgctc aggctggcga cctgtaaaat cagcgtcgcg
     9661 cccgcttccg gcgcaacggc ggtgtcggtg gaaagggcgt tgagctgttc gctggaaatc
     9721 gcctcatccg tcaccgcgaa ggtcgcctgt tccggctggc tgaccagcgg cgcgttggta
     9781 tgaaaacgca ggctctgatt gacgatatcg ttacttaatg gcgtggcaag ccacaccggc
     9841 gtgtcgttat cggccagcgt cagcagcacg ctggtggtgg cgatattcag cggctgccag
     9901 ccgcgtttga gctgatacag ggcgacaatc acgcccggct cgctcatggc ctttaacagg
     9961 cgacgaaaac tgtgctgggc atcctgcacg ggaagcataa aagcggtttc cagggtcatg
    10021 cgttgtctcc gcgaaccatc gtaaagaagt cgacccggct ggcgttcact tcggcctggc
    10081 gtgcggcaat gcgtgccata cggtcagcgt ccagcggggc aataagggtt tctgataagt
    10141 tttgaaagtg gcgagattgc tgcatcagcg catcaatcag cgcgcagcgt tcggcgtgct
    10201 gtttatcacg ccccagcacc cagctgtagc cgagggtgcc gtcagtcagg cgtacggcgg
    10261 cacgggtcag cgtggcgtcg ccggcaaaaa agcgttcgcc ggtaccgccc attcgcgcct
    10321 gaatctgcac caggccagtt tcggcagtgc ggatcacttc atagtcggcg gtgatgttca
    10381 gcgcgttcag gcgggctgcc agttcagccg gttggctgtg cgccagtacg gacatccagt
    10441 gctggcgggt cgcggtatct gcgtgcattc agtgctccat agtgaattca atcatgtcgg
    10501 cgcgcgtcag gctgacggag tactccgccg ggctgctttc accgtcacgg tggttaaggg
    10561 tgcgcacgca cagcagcggc gacatattcg ggatttcaag gcgctggcac tctttggcct
    10621 gggcgcggcg ggcgctgatc cgcgtctggc tgcggcgcag cgcaattccg gtttgctcgc
    10681 gcagaaaatc gtgcagcgaa ccgctgtcga aacgttgcag tgtcggccag agggtgaggt
    10741 ccgcaaagta gtggtcgatt aaacagagcg cgacgccgtt gacgcgacgc agggtgcgca
    10801 aatggataac gttctcaccc tcggtaatcc ccagcgcgtc agcaacgtga ccggacgcgg
    10861 ggcgcaatac cgacagcagt ttttcgctgg tgggatggct gccctggtcc agcaggttct
    10921 ggctaaaacg cgcctgggcg ttgagcgggt aatcgaacgg gcgcatcagc accagcacgc
    10981 cgatgccctg acggcgctgt acccagcctt tttccaccag ttggtcgatg gcgcggcgca
    11041 gggtgtggcg gtttacctca aagcgcgccg ccagttgctg ctcggcggga agatagtcgc
    11101 cgcagcgata gtgttgacga agctcctgct caagttttgc ggctatctcc tgatagcgtg
    11161 ttgggtagct ggtcggatgt gtagacaagt gcatagatat caatgcctca cttatcagat
    11221 aaagtgctta cgcaaccgtt gagagaggaa atccagcagg ctgaccgtga cgatgataag
    11281 caccatcagg gcgcaggttt gttggaactg gaaaccgcga atcgcttccc acagggtgac
    11341 gccgatcccg cctgcaccga ccatgccgac gacggtcgcc gagcggacgt tggattcgaa
    11401 gcgatagagg gagtaggaga tcagcagcgg cattacctgt ggcagcacgc cgtaaaggat
    11461 ctcttccagc ttgttggcgc cggtggcgcg aatgccttcc accgggccgg gttcaatcgc
    11521 ttccaccgct tcggaaagca gtttcgagag cacgccggtg gtgtgaataa atagcgccag
    11581 cacgccagcg aacggtccga gaccgacggc gaccacgaac agcatggcga agaccatttc
    11641 gttaatggcg cggcaggcgt ccatcaggcg gcgaacgggc tggtaaaccc accacggcac
    11701 caggttttcg gcgctcatca ggccgaaggg gatggagaga accactgcca gcgcggtgcc
    11761 ccagacggcg atttgcagcg tgacggccat ttcggtgagg taatcctgcc actggctgaa
    11821 atcgggcggg aagaagtcgg cggcgaaggt cgccatgttg ccgccgtctt tgatcagcgt
    11881 aagcggggcc atttccgcgc cctgccacga gacgaccaac acggcgagaa caatggccca
    11941 gctcagaagc gagaaccagc tgcgcttggg tggggcgatg gtgatggttt gcatgtttgg
    12001 ctccgtttaa tgttttctcc ctctccctgt gggagagggc cggggtgagg gcatcagcgt
    12061 gcgcatttcc ccctcaccct aaccctctcc ctcaagggag aggggaccga tcgagcacat
    12121 tactgcaccg ctttactcac cgaactcatc gcgcttaacg cgttgttcag gcggtccagg
    12181 tcatccagct gcgcctgaat ctcggtggtt ttcgccagct tgtcctgctc attcagtccg
    12241 ttattgcttt ttacgccctg aatctcttta aacagtgcga gctggcgaat cggcaccagt
    12301 tgcaggtcgc tggaggcgcg aaacggtgcc cagcccaggc gttccagcac cgctttctct
    12361 tccggcgttt ttccgtagtt cataaagaag tcgtagatct tgtctttggt ggtttcggag
    12421 agattcttgc gccagacgat cggatcgcct gggatcagcg gcgacttcca gatcaccttc
    12481 agttctttca gcttctctgg cgcggaggtt ttcagcttgt cgaggttttc ggtgttgttg
    12541 gtggcaacat ccacctgctt gttggcgacg gccagcgcgt tggtttcatg cccggcgttg
    12601 acggtgcgct taaagtcgct ggcggagata ttgtttttgg caaagacgta gtaaccgggg
    12661 acgaggaagc cagaggtgga gttaggatcg ccattgccga aggtgagatc tttccgcttc
    12721 gccagcagat cgttcaggtt gttgatcgga ctatctttgt tgacgatcaa cacgctccag
    12781 taacctggcg atccatccgc cgcgaccgtc tgggcgaaga cctggccgtt ggcgcgatcc
    12841 accgcttcca ttgccgacag gttgccgtac caggcgatat ccactttatt gaagcgcatt
    12901 ccctggataa tgcctgcgta gtctggggca aagaaggcgt tcaccttcac gcccagcttc
    12961 ttctccatat cctgtaagaa tggcgtccat tgcggtttca ggttttgctg tgattccgtt
    13021 gaaataatgc cgaaattcag cgccttttcc tgctcttcgg cgtgtgccgg gcttaacagg
    13081 gtgctgaggc tgaacatgct ggtgaaggcc agcgaggcaa ttatcttagc gttcattcgt
    13141 tttcctcaat gatggggatg tcaggcagct ttcgcgttct cttcgatgcg attaatgctg
    13201 cggtagagat ggtcaaaacg ttcgttatca aactgttggc tgctgccgtc gtagaaaacg
    13261 tgcccctggc gcagggcgac gatgcgttcg cagtagcgca gggcgtaatc cacctgatgc
    13321 agcgtgacga ccacggtgat gccgtcgttc tgattgatgt cgcgcagggt gtccatcacg
    13381 atgccggcgg attccgggtc cagcgaggcg atgggttcat cggccagaat caccttcgcc
    13441 tgctgcatca gcgcgcgggc aatcgccaca cgctgctgct gtccgccgga gagggtggaa
    13501 acgcgttgat gggcaaaatg caccatgcca acgcgggtca gcgcctgtaa cgcgcgttgt
    13561 ttctgctcgc gggtaaacca gctaaaacag gtgcgccaga acggcgtgct gccgagtgcg
    13621 ccaatcagca cgttctccag tacgctcagg cggttcacca ggttgaattg ttggaagatg
    13681 tagccggtgt tggcgcggct tttgcggata tcgcgcgcca gacggccttc gcgctggact
    13741 gtgcggccca gcagctcgat atggctgccg gcggatttat cgccggtaat caaaccgctt
    13801 aagtgacgta aaagggtgga tttgccggaa cccgacggcc caagcagagc caccatttca
    13861 ccgtgatgaa tgttcagatc aaccgcatgc agcgcctgat gctgattgaa ggttttggcg
    13921 agcttctcga cacggataat cgtttgcatg atgcagcctc cctaaaaaag tggctccatc
    13981 gtggcggatt attgtgacga tttggttaat taaaagtgac taacagatga agagttaacg
    14041 ggaattcgat gacagtgcgg gagggcgggc ctcccggcgg gttattgcgt tggttgttgt
    14101 ttgacgacat taatcatcca cggtacgcca aatttatcgg tgactttgcc aaagccatgc
    14161 gcctagaaag tttcctgcca ggccatttcg atttttccgt tagcggcaag attgtcaaac
    14221 cagcgttttc cttcttcgac ctgttgcgaa tcgagcacca gcgtaaagcc ggagtagctg
    14281 gcttttcctg acggcatggc atcgctcatc atgatgtcgc ttccggcaat gcgcacattg
    14341 gcgtgggcga tggcggtatc gggaaattgc attccggaag ggcagttctc ggcgctgtcc
    14401 tgcgctgatt ttggcatttc gccgaagctg attttataga gcagttcagc gcccaacgta
    14461 cgttgataat aagcaattgc gtcggcacag ttaccggcaa aagagaggta gggacttaac
    14521 ggcatgatgg tgacctcagt taagagaagc caattaagtg tagttcgaaa tttatacaga
    14581 tgagaggctg cctgataaga cgcgcaagcg tcgcatcagg cagtcggcat cgttgccgga
    14641 tgcggcgcga gcgccttatc cggcttaccg atcacaatac aatcagttct ttttcacaaa
    14701 ctcagatttc agtttcatcg gaccaaaacc gtcgattttg caatcgatgt tatggtcgcc
    14761 ttcaaccagg cggatgtttt tcactttggt gccaattttc agcatcgaag agctaccttt
    14821 caccttcaga tctttaatga tggtaacgct gtcgccgtca gccagcagat tgccgttagc
    14881 atctttaacg atcagctcgt cgctttcctg tgcaggttct gcgtcgttcc attcgtaggc
    14941 acattccggg cagatgtaca tgccgttatc ttcgtaagtg tattcggagt tgcattttgg
    15001 gcagtgtggt aatgacatgt ggatttcctc aaaagtcagc agggcaaaag cgccaaaaaa
    15061 cggcagattg ccgaaaaagg ccgcaattat acacaaaatc cctgagtttg tcgggggtac
    15121 tgctgatttt agacgacgat cgttttcatt tgaaaggaaa cgcaaaaaga aaataat
//
LOCUS       DAZPDU010000103        15170 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZPDU010000103 DAZPDU010000000
VERSION     DAZPDU010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15170
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.104"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(73..4533)
                     /gene="mukB"
                     /locus_tag="ACHURS_002837"
     CDS             complement(73..4533)
                     /gene="mukB"
                     /locus_tag="ACHURS_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFR1238516.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTAQRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNVVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQES"
     gene            complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="ACHURS_002838"
     CDS             complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="ACHURS_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFR1238517.1"
                     /translation="MSSTNIEQVMSVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="ACHURS_002839"
     CDS             complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="ACHURS_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFR1238518.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="ACHURS_002840"
     CDS             complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="ACHURS_002840"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFR1238519.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAECGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7458..8237
                     /gene="elyC"
                     /locus_tag="ACHURS_002841"
     CDS             7458..8237
                     /gene="elyC"
                     /locus_tag="ACHURS_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFR1238520.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTAKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEELNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8214..9107)
                     /locus_tag="ACHURS_002842"
     CDS             complement(8214..9107)
                     /locus_tag="ACHURS_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFR1238521.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="ACHURS_002843"
     CDS             complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="ACHURS_002843"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFR1238522.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="ACHURS_002844"
     CDS             complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="ACHURS_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFR1238523.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10238..11470)
                     /gene="ycaQ"
                     /locus_tag="ACHURS_002845"
     CDS             complement(10238..11470)
                     /gene="ycaQ"
                     /locus_tag="ACHURS_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFR1238524.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="ACHURS_002846"
     CDS             complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="ACHURS_002846"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFR1238525.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="ACHURS_002847"
     CDS             complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="ACHURS_002847"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFR1238526.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAIDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14275..>15170)
                     /locus_tag="ACHURS_002848"
     CDS             complement(14275..>15170)
                     /locus_tag="ACHURS_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFR1238527.1"
                     /translation="FSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQV
                     YMLDVGQGLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEH
                     LDHRGGLDSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGN
                     NHSCVVKVDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLI
                     QRVNGKVALASASRYNAWRLPSSKVKHRYQQQGYKWLDTPHQGQITVNFSAQGWRISS
                     LREQILPRWYHQWFGVPVDNG"
BASE COUNT         3560 a         4283 c         3740 g         3587 t
ORIGIN      
        1 aatgtgccac ttttttagtg cagaaacgga gttttcggaa aaagaaaagg cggcattgct
       61 gccgccttaa ttttaactct cctgagaagg cgcttcgtca cttcctggaa gcgtttcagg
      121 gagttgcggc gcaaatcctc gcaggccgac gacgtgaacg tgttcggtat tctggaagac
      181 tttacgcacc agtttatagg tggtgccttt ctccgggctg atattttccg gcgctgcgat
      241 gatgagctgc atttgcaaac gttcacacaa ttcaaacagc gtggcgatag aacgagcatc
      301 cagtcgcgct gcttcatcga ggaacagcag gcggcaagga gagatatctt taccgcgcag
      361 gcggcgagat tcatcttccc agctttgtac caccatcacc agaatcgaca taccggtacc
      421 aatcgcctca ccggtcgaca atgcaccaga ctctgcgcgc aaccagccat cggaaccacg
      481 gttaacctca acttccattt ccagatagtt gcggtaatcc agcagttctt cgccaatggt
      541 ctgcggcgtg cgctgcccca tatcaatctg cggattaaga cgctgataca atttcgccag
      601 cgcttccgag aaggtcagac ggttgctgtt aaacagatcc tgatgctgct cgtgctgttc
      661 agagagcaca tccagcagca tggcgtgcgt ttcacgcacg ttcacgttca gacgtacgct
      721 gttcacctga ccaaacgata cgttctgcaa cccctggttg agcatacgga tacggttctg
      781 ttcgcgctga atggttttgc gaatgatgtt cgccacgctg cgggaactga tcgccagttt
      841 ctgttcgcgg gaggttaact cctcggtcag tcggctaagt tcgatttcca tctgctcgat
      901 ggcttccacc ggatcatcgg tacgaataat atcctgacga atacgttcac gcagatgctg
      961 ataaaccgcc acgaagaact gaattttacg ttccggacgt tttggatctt ccgacatgcg
     1021 cagcacgtcg cgcagatgtt cgttatccgc caccgccaga cgcagcgcac ccaatgcctt
     1081 atccgacata gagcgcaaat catcagcgga gagataagcc agctcgcgac ggtgtaagcg
     1141 ccgttcaacg acgttatctt tcaccatgcg catcactgca caccagcccg ctttggcggt
     1201 cactacctgc tcgcgcatct caaagtaatc ccgctccagc ttgcgcagtt tgcgggtcag
     1261 attgtccatc tccgcttcgc agaaagtcag tgctttttcc agctggttgc ggcgggaccg
     1321 gttattactc agttgcgcat gcagctcgtc acggcgaatt cgcgcccgct cttctgcccc
     1381 gctatcggcg cgcacgccga tatcctgcaa ttcacgttgc agatcgttga gtagctcttt
     1441 tttggtgtcg taagaacttt tcagcgaagc cagcacctgg ttgtactgac tcaactgcgc
     1501 tgcgtgacca cgcaacgctt cacgagcgcg agtacgctcc gcttccgcct gttccagacg
     1561 ttcacgcagc ttttcgttga gatcgctatt accgctcagc atttctgccg agtcagaata
     1621 gctaaagtgc gcacgacgct gcaccacttc cgtcagggca aacgcctgct gacgggcatc
     1681 gcgctgcatc tgctgagagt acgcgtaatc ttcttttagc tgttcgaact gttccgggtc
     1741 gctctgcaat accgaaacga tcggttccag tttcgccagt tgattgccaa actgctgaac
     1801 aaaacgcgcg gcttcctggg cttcatccag acgttcgcgg atttcatcga cgcgatccgc
     1861 caggctgtca tcagccaaca ggttgagacg cggcagaatg cggttcagcg ccgtaacgcc
     1921 ttctttcgcc tgctcaaact gaatacgctg ctgctggtta tcattttcat gattactcaa
     1981 cgcccgctcc agttcgacgc gacggctgtt cagttgacgg atttctgctt ccgggtcaga
     2041 ctcaaacgcc accgccagat gactgccgat aaagcggctg aacgcctgat gcagacgctg
     2101 ggttttctgt acatcaaagg agagcgtggc gaagcgttcg gaaagcactt cacgctcggc
     2161 atggaggctt tcaatacggc tttcacgcgc agcacgacca aacagcggca cttccgggaa
     2221 acgtgaataa cgccactgac gatcggcgat tttcaccact accgcttttt ccagctcatc
     2281 aacgctgaac acgctgtcat cgaatgactg cggatcccct tcgatcaaat agagatcttc
     2341 cgggcaatcg gtcaagcctt ccaggtgttc agttacctgt gacagatctg gcaccacgat
     2401 ggcgtggcgt gacgggccat acagcgctga gaagtacggc gcatcttcca ggctaacgtc
     2461 gtcataaatt tctgacagca gcacgccgcc aaaacgctcc gccagcgcgt tcagacgctg
     2521 atcttcagag ccgccaggct ggcttaaacg ttcgatctct tcatcgacag cgtttttgcg
     2581 cgcgcccact tcatcgcgtt caacaatcgc ctctcgctca cgctccagca actgttgcag
     2641 atattcggtg acgtcctggc tggaggtaaa ctcttcaccg cactgctcgc tcaactggtt
     2701 gagactgttt tgcgctgcca gccaaaccgg cgcacgctgc atcaaactct gaatgcgaga
     2761 ctgcagctgt tcctgttcct ggcgcagtgc catgcgctct tcacgggcgt tagacacgct
     2821 atcggaaaga gaggcaatgc gcgcttccag ctcctgatgc agagcttcca gttcatcaat
     2881 atcaaaattc ttgccctgac gtttgcagaa gtctgccagc aggcgttcgg cttcttgctg
     2941 ctcgcgcaga cgctgttcca gttcacttaa gcgcatccgc aacggttgaa cctgctctgc
     3001 caggtgacgc tgatcgaccc cttcgcgcag cagttcgcgg gcgacatccc aagcctcgtt
     3061 acgcgccagc gggccgttga ttgccaccac cagctgataa gcctgctcaa actggctgtg
     3121 cgcggtttgc gccatgctca ttttctgctc aagagagagc attttttcag tcgcttccag
     3181 ctctttcgcc tggaaggttt ccagccattc ggcggcgctg tcggcggtta agtccggcag
     3241 atggcacagt tctttggcgc gattaagcgc agcaatcgcc tggttatact ggatcgcgcg
     3301 cgtttgctgg acgtccagcg cctgctggta gtcagcaagc tggcttttca gctcgtccac
     3361 ttccagttcg gcagcttccg cgcgagcctc gttctcttcc tggcgttcga tggcttctgc
     3421 caccacttcg ttttgctctt ccagacggat ctgcagctca tcgagatccg cttcgtagcg
     3481 ttcgatcttc tcctgctgac gctgtgcggt ttgcaccagg ttcaggtgat cgctggccgc
     3541 ctgataatcc gcttccagat cgccttcggc accgttgtgc tctgccagct cacgcgccat
     3601 atcgacgtgt ttgtactgct cagccgccag ttgctgacgc gaagtatgta actcgcgacg
     3661 aaactccagg gctttgtcga gatggacacg gcgctcgttg gcgtgacgca tgtagtccgc
     3721 cgccacgtag ttggtagctt cgctgatcag atgcttaaac aggtcacggt ccgactgggt
     3781 gacacgaatc gcttccagcg tcatgcggtt ttcacgcagc gccgcttcca tgtcctggaa
     3841 cgctttacgc acgccgctgt tttccggcaa caggtagtcg cgcagagaac gggtaatggc
     3901 actggagatc ccgccataca gcgaagcttc gatcagacga tagaatttgc tacggtcaga
     3961 tgcggagcgc agacgacgcg cgatgatgcc cagatcgaac atcagcgagt ggtaatcggt
     4021 aatggagttg aactgtttaa actgcacgcc ttccatcgcc tcgagcttgt ctttcagctc
     4081 gttaagcggc agcacgcgcg cctggcgttc gttcagggtt tcggtcacca gctgtgtcgg
     4141 ctgcaccgac atcggcagtc cctgaatggc aaatggtttg atatccactt tacgatcgcg
     4201 tccggcaacc tgttgcagac gcacaccgac caccacgcgc tggtggcgcg agttaatggt
     4261 gtcgagcatc gaataacaga cacccgcttt cagcttaccg tgcagacctt tatcgcgcga
     4321 accgctggtg gccccggctt ccgtagtgtt acggaaatgc agcagggtca ggtcggggat
     4381 cagcgccgta acgaacgccg ccatggtggt ggatttaccc gccccgttac cgccagaaag
     4441 cgtcgtgacc agctcgtcaa ggtcaaaagt tcgggcaaaa aagccgttcc agttaatcag
     4501 cgtcagtgag cgaaatttac cgcgttcaat cattattctt cctctccgct atctggctga
     4561 ttctcttcgg tttcatcgtt gagttgcaga tgattttcaa tcggcattgc ttcgccatca
     4621 cgaatcaggc gacgctgtgc ttcacgggga tcgtcgccag cacgcacatc ggctccgaag
     4681 cggaacaccg attcggtaat gcgaaacttg ctgctgtcgt ggcccataaa ccacaccatg
     4741 cctaaacgac gcagacggtt gagcgaagaa cgtactttct cctgcaactt ctgacggtca
     4801 acgtctgaac cggttgaacg gttgttcacc agtttcagca gttttgcttc atcggccagg
     4861 gtgagcagtt cgtcgtacag ttcctgctgg gtgaaaatcc cctcattcgc cagccgttcc
     4921 gggctgagat agagataaca gaggattttc ccgaccatca tatccagttc cgacaagacg
     4981 gaacgcggga taagagtggt ggaacgtggg cgtaaataga agaacccttc cggtgcgcga
     5041 ataagctcaa cgttataacg cgcgtaaaac tcttccagat attcctgaaa atccatcagg
     5101 aatgcatgat tatccagttc gtcgaggcca atatggcggc ctgaacgtaa ggcgctgtcc
     5161 agcgccggaa ataacggatt cgccagcgcc tgcgccagct taaccgacat cacttgttca
     5221 atatttgtcg atgacatgcg cctgtacctt ggctccgtaa tcattaatcg gctgccattt
     5281 cgctggcagt ccggtgaaat ctgcttgcgc tacgccaaga cgtaccgcct gatcaataac
     5341 aatacgcgca acgtcaaagt gacgtgcacg cggatactgt gacagatatt cgcgtaccac
     5401 cagaccaaga tccagcggca cttgtctggt tttgtacacg gcaagttgtt cttcgatgat
     5461 cgccgccagc tgttcgcgga tctcgttaaa ctcttcgtat tccagatcct caggaagttc
     5521 cccagtcact tcttcatcgc gcagtgccat ctcttcgtca cgcatatcca gcagacgatc
     5581 ggcattggca taagttagcg cccacggctc atcaaaatag gtttgtaccg actgacgtaa
     5641 ccgctgagca aagacgcggt ttttatccat atcgatcgcg gtacgaataa atttgtgtac
     5701 gtggcggtcg tagccaatcc acaagtcgat ggattgctgg ccccaactga taatacgatc
     5761 gagtttgctc tgcagatcga acaccagacg atcgacgaaa tgcagatcgt catgggtcat
     5821 cgtcgcatcc tgaatgcgca acagattagc ctgcaatttg tcgcctgccg cttccagcgt
     5881 atcctgcaat tcacgcagcg ttccggaagt ttccgaaagc aacaattcac agctggaaat
     5941 cgccgcccgc cagtctttgt tcagcaactg ggcgatatcg tccttcacct gctgctgctg
     6001 ttcgtccatc agacgttgcg tcaggtcgat actgtcgaaa atttctgcta ccgaatattt
     6061 cagtggcgca tagacattac ggtgccagtg aaattcatca ccgccctctt cggcggcatc
     6121 tgctgcgcgt ttgagctcac ccgccacaat cgacaactgc atagaaagac gcagcgtaga
     6181 aaactcgcgc tgacggatgt agtagtcagt aatgccgatg ccgagcggcg tcagacggta
     6241 aattgcgttc ccttccgcct gctcgctggt aaagcggttc agcagacgtt gacgcaccat
     6301 gtcgttgatc gcgttattgg cgcgcacgcc gatggtttcg ctggtttgct caaacgcatc
     6361 actcacatgg cggaatgcat ccaccagctc gccttcactc atctcaccat ccagacgctc
     6421 gccgttcagc gtggcaaccg ccagcagaaa agagagtcgg tctaccggca gcgagatgga
     6481 gaagtcattt tttctggccc aggcaaccag ttcggggact gtctgggaaa attcactcat
     6541 actttatcct tgctctgcgg tttgcgcgcg gtgacatgaa tataacgccc cagggtaata
     6601 tacggttcct gacggcaata acgcgtttct aattcaagta atgcttcata gcagtcgcgc
     6661 tgctggtgct tctcgcgcag ataatcatga aacacgcgaa cgcctgtctt acccataatt
     6721 tgccaaccag cttcttccag ccacagataa acctgcgccg ggtcgcgtgg ataatctggc
     6781 gaaagcgtcc gttttttctt tttcggcatt cccgcctgca cgtaatcaaa attcccggcg
     6841 accatgttat gcatcaacaa accatgcgca ttgtagaaca ttaacgacaa cacgccgcct
     6901 ggacgcaaca ctgaccacag ggtctgcaat acactgcggg gatcggccac ccactcgagc
     6961 accgcatgga acaatatcag atcaacgggc gtttccaaat gcgaagcaac atcctgagcg
     7021 gcgcaatgta taaattgcat gttgtcgctc acaccttttg cttctgccgc ctgttttgcg
     7081 cggtcgatca tctgcgcaga aagatcgcat aaaatgacct gatgcccgca ctcggccatt
     7141 ttgattgcgg tctgcccttc tccaccgccc gcatccagca cacgcagttt ttgcggaccc
     7201 atttccgcca gcacgcgatc gagatcctgc cacagaatag cctgtcgaag ctgccctttg
     7261 gtggtgccgt aaatgttacg ggaaaacttt tccgcaatat catcaaaatt gcgatcctgc
     7321 attgctttct ccaccggttg gctaagacgt tttctgataa agacgctatt ttgtcacagc
     7381 cacggcgaga atgaacccgt ctggtgtaat atcgctggcg atcccttgct atatggttaa
     7441 aaaaggaacc agaaaggatg ctttttacac tgaaaaaagt gattggcaat atgttgctgc
     7501 cacttccgct gatgttgtta attattggcg ctggcctggc gctgctgtgg tttagccgtt
     7561 ttcaaaaaac cgcgaagatt tttattagca ttgggtggct ggcactcttg ttattgagtt
     7621 tgcaaccggt agccgaccgc ctgctgcgtc ccatcgaaag cacctatccg acatggaata
     7681 attcgcaaaa agtggactat atcgtggtgc tcggaggtgg ttatacctgg aacccgcagt
     7741 gggcaccgag ctctaattta atcaataaca gtctgccacg cctgaatgaa ggtattcgct
     7801 tatggcgcga aaatccggga tcaaaactga tcttcacggg aggcgtagca aaaaccaata
     7861 cggtgagtac agcggaagta ggtgccagag ttgcgcaatc gctgggcgtg ccgcgcgagc
     7921 aaattatcac cctggatttg ccaaaagata ccgaagaaga agctgcggca gtgaaacagg
     7981 cgattggtga cgcccctttc ctgctggtga cctccgcatc acatctgccg cgggcgatga
     8041 tcttttttca gcaggaagag ttaaatccgc taccagcccc ggcgaatcag ttggccatcg
     8101 actcgccgct taatccgtgg gaacgggcaa tcccttctcc ggtctggctg atgcatagcg
     8161 atcgtgttgg ctatgaaacg cttggccgca tctggcagtg gctgaaaggt ccgtcaggcg
     8221 agccacggca agagtgattt tgatgccaga tcgaagttgc gccgactaaa tcgtccggta
     8281 ttaaccagtt gcgcaacttc atcccataac acatacaacc aacgccgcca gatgaacgac
     8341 tccgccactg gcgcgcgttg caggtaactc cagagcaaat cttccgccag agaattatcc
     8401 attagtcgga acagttcgta ttctcgcggt gcccaaagca ttagtcccgg cccgaccatc
     8461 gccagtaact gatcgctgcg cgaatctttc agcatgctgc gtaaacagaa gttaccgtga
     8521 atcaacacac aattgtcgtt aaagccttca aataatgccg ggagacattc gcgagtgcga
     8581 aacaggatcc gcttatcctg catcgtcaga ccggtgttat tgaactgatt gagcgtggtc
     8641 catagcactt caacatgttg ccggtaccat gagggccaga aattttcctg agtattgtcg
     8701 accgcgccga cgcaaccgcg actgtcctga cggtgccagg ccagtaaggc ttcaacgatt
     8761 tggtctttga gctgttccca gcgttctggt gttcgggctg gtgcctccac cgaaacacca
     8821 cgcatccgct ccagtagcag gacatcaggg ccggggtgtt cttcatgcgt catcacacca
     8881 taaatagtcg gcatacggac agtcccactt cttgccagca tggtggtttt ccatgccagt
     8941 tgtcgggctt ttccgggcgt actaaagctt cttgccatta acggcattgg gtttccctgg
     9001 ctgtcataca aagcccacaa cgccgtatcc gctttttcat tgacgcactc aatacgactg
     9061 agtttttcgc ccagtaaatg gcttaattcg gcacgcagct gttccattca gattaccctc
     9121 ataaaagcta ctgctttcat aatgagcgcc aatataaaag atgtcacctg aatgagatca
     9181 attgttgaaa agaaaaattg aggaaaatcg taacggtacg acactcctcc caaaattggc
     9241 tgaagtgtcg tgaagtgaaa ttagcgcatt tcagcgcgaa cgcgctcaag atcttcaggg
     9301 gtatccacac ctgtgccagg aacttcctga gcaacagcaa catggatttt ttcgccgtac
     9361 cacagaacac gaagctgctc taacatttcg atgtgttcta acggacttgc ctgccagttg
     9421 acgtaacgac ggataaagcc tgcacggtag ccataaatac caagatgacg caggaagtta
     9481 tcgccaacgg tttcaaggcc ttctgcaaaa cgatcacgat cccaaggaat ggtggcgcgt
     9541 gagaagtaca gtgcataccc ttcagcgtcg agaaccactt tcaccgcatt cgggttaaac
     9601 gcttcttccg cattgtggat tggcaccgcc agagtcgcca tacccacctg acgctgagcg
     9661 aggttatcag caacctgacg aatgattgtc gcagggatca tcggttcatc accctgcaca
     9721 ttaacgatca ccgtgtcgtc gctgaatgcg catttttcga caacttccgc cagacgttct
     9781 gttcctgact gatgatcggc gcgcgtcata catacttcac cgccagcggc ttcaacggcg
     9841 cgggcaacat cctcatgatc ggttgccacg atgatgcgct cggcacctga ttcacgcgcg
     9901 cgttcaagaa catgaacaat catgggtttg ccgttaatat caaccaatgg tttaccgggc
     9961 agacgcgtgg acgcgtagcg cgcgggaata atgaccacaa aactcatgat ttactctcat
    10021 cagcagtcag cacgcgggct tccgtttcca gcaacactgg aatgccatcg cggagcggga
    10081 aagccaggtt atccagtttg caaataagct cttgtttttc ctggttatac caaagttttc
    10141 cgttgcaaac cgggcaggca atgatttcaa gcaaacgatg atccatagtt cctccagatg
    10201 gatcgggtta tgaatgcata aatcttatca taatcattta tgcgacgggg tctatttccc
    10261 agccagtgcg gcaatcagta aagagacctt gcgggcagcg tcctaatgtc acccgcgttg
    10321 cctgctgcca gctcgcgaaa tcagtaatcg cctgacgtaa ccctttttgc agcattgtcg
    10381 ttggtttaat gccttcctgt aaccacagag agataacttc aaggatgcct gtctggcgat
    10441 gcattttggc atccattcgc ccaactaatt gcccacgatg taataatggc agaacaaaat
    10501 agccatactg gcgtttcggc gctggggtat agcactccag ccggtagcta aaatcaaaaa
    10561 gctgctctgc gcgtttgcga tcccagacaa caggatcaaa aggcgaaagt accgcgctgt
    10621 gcgttgcagt gagctttccg gccagcgcac gctcgagtag cggcagcaaa tcagcatgca
    10681 gccaaagatt gcccaatttt tcaacatgca cagcaatgat ttgctgctgt tcagccctcg
    10741 cttcgcgcca cgccgccagt gccggacgtt tcagccgata gtaatctgcc agccactgtt
    10801 cgcggaatat tcccaggcta cgcgcactgt tatccagcat gatgatttct gcttctgttt
    10861 gcgaaacgag atcgcgctca tcatcccagt caggcatgac acggtgggtt aaatcataaa
    10921 cgcgctggaa gttgcgccgt tcaatcacca tcacctttcc ggcagtaaat aaaccttcca
    10981 gatgccgttt atgcggcttc cattcccacc agccgcttgc acctttacga ggatgctcaa
    11041 aatcggctga acgtaccggc cccttatcat gaatatgctg aattaactgt gcaatttccg
    11101 cctcatgttc ctgcatccag gcgtctttgt atttccagcc cattttttca ggtgccagca
    11161 tgcggtggcg aataagacga aagtcgctac gcggcataaa gcaggcttca tgcgcccagt
    11221 attccattaa ttcgccacgc gccagagact catccagcca ctgggcagga taatttccca
    11281 gacgactgaa aagcaccaga tatggactac gggcaacaat attgatggta tcgatttgca
    11341 gcaaggacat gcgggagatc gttgccggaa tatcctccaa cgacgctcga cggcggggtt
    11401 tgtttaacag gccttgtgcg gcaaggtgaa gattacgcgc atcagcaagg gagaggtgcg
    11461 gcagtgacat tcatgactcc atcaatcgaa cgctgccgcg gcgtaactag ttgccagaag
    11521 ccagcgaggt tagttgcgta agcagtttcg ctggttcatc acctgaaagc tgtgcgtcta
    11581 caggcaaata ccaccaattt tcttctgcaa aggcccggca tttcaccgca tctttttcag
    11641 tcattaccag cgtttgcccg gcgcttacca acgcactgac atccgcatgg ttcaaagact
    11701 gatgatcggc cagcggtaca catttttccg gttgtacgcc acacatcttc agcgtggcaa
    11761 aaaagcgcgg cggatgccca atccccgcca tcgccactac atgttcaagc tgagcaacgt
    11821 cacaacgcgt accggtacgt aaattcaccg cctgacccgg cagcagatgc atggggattt
    11881 caccgctgcg agggacaccg ccgttgacga ttaccgcatc aaccgacttt aagcgccccg
    11941 ctcgctcacg cattggcccc gccggcaacc accagccatt gccaaagcga cgcacaccat
    12001 caataacgac aatttccaca tcacgcgcca gacggtaatg ctgtaaaccg tcgtcggtta
    12061 cgatgatctg cacatcaggg tgttgcgcca gaatggcttt taccgcatca gaacgaacgg
    12121 gagaaaccgc aacaggcgca tcagtgcgtt gataaatcaa cacaggttca tcacccgcct
    12181 gtgctgttgt ggtatctgcc gacaataaca gcggataaga ttcagcctta ccaccatatc
    12241 cccgcgatac gacccccacg cgaataccgc gctgttgcaa ctgttccacc agccagacaa
    12301 cgaccggggt ttttccgttg ccgcctgcgg tgagattacc aaccacgaca accggtacgg
    12361 gggcacgcca ggcgcgcttc agttttagtt tatagcaaag acggatcgcg ccactcacca
    12421 ggccatacaa ccaggagagt ggcagcaata gccgccacaa aggggattca ccagaccaga
    12481 ttttttcgat cattggccaa actgcatttt gtgaagttgc gcgtaaacgc cgcggtgctc
    12541 aagcaaatcg ttatgcgtac cgcgttccac aatgacacca tcctcaacga ccacgatttc
    12601 gtctgccttt tcaatggtag acaagcggtg ggcaatcacc agagaggtac ggtttttctg
    12661 caactcatcc agtgccgcct gaatcgcacg ttcggattcg gtatccaacg ccgaggtagc
    12721 ttcgtccaga atcagaatcg ggctatcacg caacaaggct cgagcgatag caatacgctg
    12781 acgctgaccg ccagagagca gcacgccgtt ttcaccaatc actgtatcga gaccgttatc
    12841 catcttattg atgaagtcta tggcgtaggc catacgcgcc gcttcttcaa tttgctcacg
    12901 gctgtactgt tcagtccgtg cgtaagcaat gttgttagca accgtatcgt taaacagatg
    12961 gacattctgc gacaccagag caacctggtt acgtaacgac gccagggtat actcgcgcag
    13021 atcgtgacca tccatcagga tttcgccttc atcaatatcg taaaaacgcg tgatcaggct
    13081 ggcgatggtt gatttacctg aaccagagcg tccaaccaga gcgaccgttt tccctgccgg
    13141 aattttcagg ttgatgttac gcaatgcggg cacgtcacgt cccggataag taaaggtgac
    13201 attgcggaat tccacgtcgc cagtcgcacg ctcgatcacg cgcttacctt cgtctttctc
    13261 ctgctcactg tccagaatgg taaacagcgt ctgacaagcc gccataccgc gctggaactg
    13321 ggcgttaacg ttggtcagcg atttcagcgg acgcatcagt gcaatcattg aagagaaaac
    13381 aacggtaatc gtaccggcag tcaggttatc cataacactt gggaagctcg ccgcatacag
    13441 aacaaacgcc agcgccaaag aggcgattag ctgaatgatc ggatcagaga tggaagaggc
    13501 tgaaaccatt ttcatcccct gaagacgcat tcggttactg actttatcga agcgtttcgt
    13561 ttccacttcc tgaccaccga aaatcaatac ttctttgtgg cccttcagca tttgttctgc
    13621 gctggtggtc acctgcccca tggtgttctg catgttttta ctgatgttgc gaaaacgctt
    13681 cgatacaacg cgaatcgcaa tcgaaacaat cggtgccagc acaatcaaaa tgatcgacag
    13741 ttgccaactg taatagaaca tcatgatgaa caggccgatg attgacgcac cttcacgcac
    13801 aaccgttatc agtgcgccgg aagaagaaga agcaacctgt tcggaatcgt aggtgatacg
    13861 tgacaacagc gtacccgttg actgtttgtc aaaaaatgaa accggcattc ccatcatgtg
    13921 accaaacagg cggcgacgca tggtcattac cacctttcct gatacccagg agatacagta
    13981 gctggagaca tagctggtga taccacgtaa aatcatcagc ccgatcacca ccagcggcat
    14041 ccacaccagc acggagcgat ctgttttacc aaagccatca tcaagaagtg gcttaaggag
    14101 cgataacatg aaggtatcgc tggctgcgtt gaggattaac gctacgcccg ccacgatcag
    14161 acccgcttta aaaggcgcaa tggttggcca cagtcggcgg aacgtctgcc acgtagagag
    14221 atctttgtcg ttatgcattc aaaaaaccag catttgttga aatagccgca tattctaccc
    14281 gttatccact ggcacgccaa accactgatg ataccaacga ggtaaaattt gctccctgag
    14341 gctgctaatc cgccagcctt gcgctgaaaa attgaccgtt atttgcccct gatgaggagt
    14401 atcaagccac ttataaccct gttgttgata gcgatgttta actttgcttg agggcaatcg
    14461 ccatgcgtta tagcgcgatg ccgatgcgag tgccactttt ccattcactc gctgaattaa
    14521 tggcaacgat gatgaggtat tactgccatg gtgaggtacc tgaagcaatg ttgtctgcac
    14581 ttgctgccag taacggctta gcatcttttg ttcagcgggg acttcaatat caccggttag
    14641 aagaatgcta ttcgtcccgt catcaacctt aaccacacag gaatggttat ttcctttatc
    14701 gttgctagct tgtaaaggcc agtgcacgct gaaacgcaat ccttgccatt gccacgcttc
    14761 gccacgcaca cagggctgat gatgttccca gtttaacgga cttctgatcc ataacatcgg
    14821 ccatgtgtgc aatattgagt ccagccctcc ccggtgatcc agatgttcat gactcagaat
    14881 aacgccttcc ggttcaagat tatgccagtg gagccagggg ataatcagtt gttgcccact
    14941 atccccttcg ggccaggcca gtcctgtgtc atagagaatc gctttgccgt ttctggctat
    15001 caccattgcc agcccttgcc cgacatcaag catgtacacc tgccactcgt caggtcgagg
    15061 tttttgccac agcggccagc acatcagcaa gcctacagcc acacacattg ctggcaacgt
    15121 tcgccaggcg tttaatcgcc ataccaccag taagaaccat ggggaaaatg
//
LOCUS       DAZPDU010000104        15143 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZPDU010000104 DAZPDU010000000
VERSION     DAZPDU010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15143
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.105"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(30..443)
                     /locus_tag="ACHURS_002849"
                     /pseudo
     CDS             complement(30..443)
                     /locus_tag="ACHURS_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            1030..1377
                     /locus_tag="ACHURS_002850"
                     /pseudo
     CDS             1030..1377
                     /locus_tag="ACHURS_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
     gene            1430..1825
                     /locus_tag="ACHURS_002851"
     CDS             1430..1825
                     /locus_tag="ACHURS_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFR1238528.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            1866..2609
                     /gene="cmoA"
                     /locus_tag="ACHURS_002852"
     CDS             1866..2609
                     /gene="cmoA"
                     /locus_tag="ACHURS_002852"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFR1238529.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCSRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            2606..3577
                     /gene="cmoB"
                     /locus_tag="ACHURS_002853"
     CDS             2606..3577
                     /gene="cmoB"
                     /locus_tag="ACHURS_002853"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFR1238530.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(3742..6171)
                     /gene="torZ"
                     /locus_tag="ACHURS_002854"
     CDS             complement(3742..6171)
                     /gene="torZ"
                     /locus_tag="ACHURS_002854"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFR1238531.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKDGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTTRIQHPMVRKSYLDNPLQPT
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIDFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLRGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMRAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(6196..7296)
                     /locus_tag="ACHURS_002855"
     CDS             complement(6196..7296)
                     /locus_tag="ACHURS_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFR1238532.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPGLADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(7684..8430)
                     /gene="cutC"
                     /locus_tag="ACHURS_002856"
     CDS             complement(7684..8430)
                     /gene="cutC"
                     /locus_tag="ACHURS_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFR1238533.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(8444..9010)
                     /locus_tag="ACHURS_002857"
     CDS             complement(8444..9010)
                     /locus_tag="ACHURS_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR1238534.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSLRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLLGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            9226..10962
                     /gene="argS"
                     /locus_tag="ACHURS_002858"
     CDS             9226..10962
                     /gene="argS"
                     /locus_tag="ACHURS_002858"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFR1238535.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRHSAKVQFGDYQANGM
                     MAVAKKLGMTPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGDEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKL
                     QSGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAK
                     GLAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRV
                     LYYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLA
                     DLLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNM
                     LAFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETL
                     TVVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLG
                     LDTLGIETVERM"
     gene            complement(11004..11048)
                     /gene="yecV"
                     /locus_tag="ACHURS_002859"
     CDS             complement(11004..11048)
                     /gene="yecV"
                     /locus_tag="ACHURS_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecV"
                     /protein_id="HFR1238536.1"
                     /translation="MSIFRIHLDGNKKA"
     gene            11139..11627
                     /locus_tag="ACHURS_002860"
     CDS             11139..11627
                     /locus_tag="ACHURS_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFR1238537.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIENDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(11747..12139)
                     /gene="flhe"
                     /locus_tag="ACHURS_002861"
     CDS             complement(11747..12139)
                     /gene="flhe"
                     /locus_tag="ACHURS_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFR1238538.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(12139..14217)
                     /gene="flhA"
                     /locus_tag="ACHURS_002862"
     CDS             complement(12139..14217)
                     /gene="flhA"
                     /locus_tag="ACHURS_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFR1238539.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(14210..>15143)
                     /gene="flhB"
                     /locus_tag="ACHURS_002863"
     CDS             complement(14210..>15143)
                     /gene="flhB"
                     /locus_tag="ACHURS_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFR1238540.1"
                     /translation="DPNLILGQIILLIREAMLALLPLISGVVLVALISPVMLGGLVFS
                     GKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTGFYLWHHWPQMMRLM
                     AESPTTAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKKLRMSRQDIRDEFKQ
                     SEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVALQYDENKMSAPKVVA
                     KGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQLYAAVAEVLAWVWQ
                     LKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
BASE COUNT         3730 a         3981 c         3584 g         3848 t
ORIGIN      
        1 tttacaggtc ttcccatatg taccccttaa gctttagcac gttcaatacc agcctttcta
       61 cgctctgcta tcaagcttct ttcaagctgt gccaacgttg acataatgcc taacattgct
      121 tcacccatag cgcttgataa gtcaaatcct tctctaaggc tgataactgt cacaccttta
      181 gcctgtagcg ctttaaccgt tgataacaca tcaattgtgt cacgtccaag acggtctaca
      241 gctaccacaa ctacggtgtc accttctcgt gcataagcta acaaactgct gaaaccgttt
      301 cttaaagaag ctttgacaga accactgaca gcttcatcag cgaaccattt agcaatgttg
      361 taccctgcct cttcaatttg catttgctgg ttagtgatgg attggtcatc tgtactcacc
      421 ctgcaatatc caagtatcgc cataatgttc cccttagaag tctcaaaagt tagtctcata
      481 agtttagctg tctcataatc caaaaacaac cttttgagac tacttgaggg tgcattctac
      541 cgttacgttg aaagtgtctc aaaaggactt ccttatgaga ctggttaata gagcgctatg
      601 aagattcagg agggtaacta taaccatcac tttctaactt tcacgtagga aatcgttaag
      661 tttcttgata gttacaccat gtgaccaact atctatatat cgttatatag tcctgtatat
      721 agcactatat gtagtgtctg gagagggacg actaacacga tacagtttac cctgaaaaga
      781 gacttcacag attctttata tatttcaata agttatgcta aaaagagact ataccgatac
      841 tgcatagaat ttgtgtagga ttgcatagat acttggaaaa acaccgttaa attgcatagt
      901 cattcacctg atattgtcct gtttttacag gtagttgatt cggatgttct aggaatcatc
      961 ctcatagttg attcggttag ttgggcttgt accaacccct aatcaacctc atagtgtatt
     1021 tagaccttga tgattttaga cagccgccct gttcatgcag cacgtccaca cagtgaagct
     1081 attcgcgacg ctcaacgaaa aaaacctaaa gttccggtac atgctgtact gacggcgaca
     1141 aatccgctga ttcgttttat cggtagtgat gatatgacgc aaaaccggga attatttcag
     1201 gtctggttac agaaattagc gcagtggcat cagaccacta cgccttatct ttttttacat
     1261 acgccagaca ttgcccaggc cccggaactg gtacataccc tgtgggaaga cttacgtaaa
     1321 acgcttccag agatcggagc agttccggct attccacagc aatcttctct tttctgaatt
     1381 tgccacctat catagacagg tgccatcggc cattttaaag ggagtttgta tggtaagcgc
     1441 gctatatgcc gttttaagtg cgttgttatt aatgaagttc tcttttgatg tcgttcgcct
     1501 gcgaatgcag taccgcgttg cctatggtga cggtggtttt agcgaactgc aaagcgctat
     1561 tcgcattcat ggtaacgcgg tggaatatat tcccatcgcg attgtgctga tgctgtttat
     1621 ggaaatgaat ggcgcagaaa cctggatggt gcatatttgc ggcatcgttt tgcttgctgg
     1681 tcgtctgatg cattattacg gttttcatca ccgtctgttt cgctggcgac gttccggcat
     1741 gagcgccacc tggtgtgcgc tgttgctgat ggtgctggcg aatctttggt atatgccctg
     1801 ggagttggtt ttctccctgc gttagcgcac aatacgccac tttctttttc ccggattttt
     1861 acgttatgtc tcaccgcgac acgctatttt ctgcccctat cgccagactg ggcgactgga
     1921 cctttgatga acgggtagct gaagtcttcc cggatatgat ccagcgttcc gttcccggct
     1981 attccaatat tatttccatg attggtatgt tagccgagcg cttcgttcaa cctggtacgc
     2041 aggtttacga tctgggttgt tctctgggcg cggcgacgct ctcggtgcgt cgcaacattc
     2101 atcatgataa ttgcaaaatt attgccatcg acaactcccc ggcgatgatt gaacgctgca
     2161 gtcgtcatat tgacgcctat aaagccccta cgccagtaga cgtcattgaa ggtgatattc
     2221 gcgatatcgc cattgaaaac gcatcgatgg tggtgctgaa ttttaccctg caattcctgg
     2281 aaccttccga gcgccaggca ttactggata aaatttatca aggactgaac ccgggcggcg
     2341 cgctggtgct ttcggaaaaa ttcagtttcg aagatgccaa agttggtgaa ctgttgttca
     2401 acatgcacca cgactttaaa cgtgccaacg gttacagcga actggagatc agccagaagc
     2461 gcagcatgct ggaaaacgtg atgctgaccg attccgtgga aacccataaa gcacgcctgc
     2521 ataaagccgg ttttgagcat agcgagctgt ggttccagtg ctttaacttt ggttcactgg
     2581 tggcattaaa agcagaggac gctgcatgat cgactttggt aacttttatt ctctgattgc
     2641 caaaaatcat ctttcacact ggctcgaaac gctgcccgcg cagattgcta actggcagcg
     2701 cgagcagcag cacgggctgt ttaagcagtg gtccaacgcg gtggaatttc tgcctgaaat
     2761 taaaccgtat cgtctggatt tattgcatag cgtaaccgcc gaaagcgaag agccactgag
     2821 caccgggcaa attaaacgca ttgaaacgct tatgcggaac ctgatgccat ggcgcaaagg
     2881 gccgttctca ctgtatggcg tcaacatcga taccgaatgg cgttccgact ggaaatggga
     2941 tcgtgttctg ccccatcttt ctgatttaac cggacgcacc attctggatg tcggctgcgg
     3001 cagcggttat cacatgtggc gcatgattgg cgcaggcgcg cacctcgcgg tggggatcga
     3061 ccctacgcaa ctgtttctgt gccagtttga agcggtgcgt aaactactgg gtaacgatca
     3121 gcgcgcacat ttgttaccgt taggtattga acagcttccg gcactgaaag cctttgatac
     3181 cgtcttttcg atgggcgtgc tttaccatcg ccgttcgccg ctggagcatc tctggcagtt
     3241 gaaagatcaa ctggtgaatg agggtgaact ggtgctggaa acgctggtta ttgatggcga
     3301 tgagaacacg gtgctggtac caggcgatcg ttacgcgcaa atgcgtaatg tctatttcat
     3361 cccttccgcg ctggcgctga aaaactggct gaagaagtgt ggttttgtag atatccgcat
     3421 tgtggatgtg tgcgttacca ctactgaaga gcagcgacgc actgaatgga tggttaccga
     3481 atctctgtcc gatttcctcg acccacatga tccgagtaag acagttgaag gttatcctgc
     3541 gcctaaacgc gcggtgctga ttgcgcgcaa gccgtaaagg tctggtatta ctgccggatg
     3601 cggcgtgaat gccttatccg gcctacaaac ccttgctaat tcaatctatt gcagggacta
     3661 tgtaggcctg ataagcatag cgcatcaggc agttttacgt ttgcataacc tcagcgcccg
     3721 tttccgggcg ctattcactt cttacgcctg aacagcaggc ttatcaaatg ccgttaactt
     3781 cggtgcgtta cccgtatatt tttcgatata caccagcgca gagttaccgg cacaggcatt
     3841 tgccagctgc gagctgggga tatccgccgt taacacgttc gcactgccgt ttttacacaa
     3901 gccgttttcc agatctggcc atgcaccttc atgcacgcat accacgcctt ttttgatccc
     3961 gtcagtcacc accgcgcctg tcaaaatctg accacgtttg ttccagacac gcaccagatc
     4021 gccattcgca ataccaaatc gagcagcatc ttcggtgtga atagtgatcg gttcacgatc
     4081 ggcaaccgca tattttttac gcagttccgc atagttaagc tgactgtgta aacggtgtgc
     4141 cggatgtgcg gtcagaagct gcaactgctt ctcgtcggcg gtacccttcc actcatcagg
     4201 cgcaagccag gttgggtgtg ccgggcaatc cttatagcca aatttttcca gcgttttgga
     4261 gtaaatctca attttgccac ttggcgtacc cagcgcattt ttcaccggat cggcgcggaa
     4321 atcaccataa cgaacgtact gttcgttctt ctcgctgcgg cgcatttcga tcagtttatt
     4381 ttgctgccag aaggcattaa acattggcat agtgacgcgt tgcgcacggg cccctttctg
     4441 agcggcatca tagaaaaatt tcagccacgc catttcatct ttaccttcgg tatagatctc
     4501 ttttccgcca ggtttgagta attccgcaag atcggcaaac acatcaaaat cgtttcgcgc
     4561 ttcaaattgc ggagcaacag cctgcttcat cggcacaata tgctggttgc tgtaatcacc
     4621 ggtcatcgtc aagtcattgc gctcaaacga tgtggtgatc ggtaatacga tatctgcgtg
     4681 tttagcagct gcggtccagt agcattcaga aacgacgatc atctccggtt tctgccatgc
     4741 tttaatcaga cgattagtat cctggtgatg ggtaaagtta ccgccacccg cccaccagat
     4801 cattttgata ttcggatacg tttgctcttt accgttatgc tgatatttgc cgccaggatt
     4861 ttccagcgca tcgacgatac gggcaacagg aatcgccctc attccgccat catcagcggc
     4921 ctcgctggcc tgtccggcaa tcgccgcaga catttccggc agcacgccgc caacacgtgt
     4981 cgggttgcca ccattggagt aatgataaga gaaaccaaaa ccgccgcccg gtgtaccaat
     5041 ctgccctaac atcgccgcca gcgtcaccag catccagtgt ttctgttcac catattgctg
     5101 gcgctgaatt ccccagcctg ccatcagcat tgtgcgattc gccgccatca gttcagcgag
     5161 tttgactatc tgcgcttccg gtacgccagt aatttccgcc gcccaggccg cgctcttcgg
     5221 cgtattatcg ctcttacctc tcagatactc ttcaaactgc ggatacccgg tagtgtattt
     5281 ttcaaggaac actttatcgt gtttgccttg tgtcatcagg gtatgcgcaa tccctaacat
     5341 cagtgccacg tcggtgccca tattcggcgc gatccaggtg gcattatcgc caaagaagtc
     5401 gatggtttcg gagcggatag gatcaatggc aatcactggt ttgccagatt ttttcagctg
     5461 atggaagtat tccagccctt gctcatcggt actgctccag gcaattttta aggtattcag
     5521 cgggttcatc ccccacagca ccacaacctg gctgttttcc agaatcagcg gccaggaggt
     5581 ctgctgttca tacacctcta cagaaccgac cacatgcggc atgatcacct gtgccgcccc
     5641 ggttgaataa tcgccgctat gcccggaata accgcccgcc aggttcatat aacgttgcag
     5701 taaggtttgc gctttatgca acacgccaga agagcgccag ccgtatgatc cggcaaaaat
     5761 agccgatggt ccgttagctt tgcgaatgcg atcgtgttgc tcatgaatca gttttaatgc
     5821 ttgttcccag ctcacctgta cataggtatc ttcgccacga cctttcgtcg gttgcagtgg
     5881 attatcgaga tagctttttc tcaccatcgg atgctgaata cgcgtcgtgg tgtgtacctg
     5941 atccgccgcc gtagactgta aggaattcgg tatggttttc gccagcgcgc ctgttgaaga
     6001 aacaatcttg ccgtccttca cttctacgtt catcgctccc caacgtcccg cggtgaggat
     6061 tttaccgtcc ttctcttctg cccatgcggg gagcggtgct gccgatgtca ccaccagcgc
     6121 tccagcggca ataccgctgt gtttaataaa ttcacgtctt gttaatgtca taacttcctc
     6181 cctgatcaac gaggatcact gtttctcggt aatatctttg gcgttgtact ggaaataccg
     6241 cgttaaaatg tccagttcgt tttcgctcat gctggttcgt gcccccattc ctttggcaat
     6301 ggacggccac gcattgacgg tgtaatggtc ggcggcaata ggggcatgac aaccagcgca
     6361 ataggtatcg tcaagttttt cagcgtattg ccatagcggt ttacggtccg ctaatccggg
     6421 atcggtaagc gcaccctgta aagacgcctg acgccattga ttgccgtatt cgtcagcctg
     6481 ccattccccg tttaccgtga gcgccttgat accttcttca cttaatgtgg ctagcgccag
     6541 ccgttgacct gccgccaggt agagcgtgtt ttcactgccc tgcatttgat aaccctgcaa
     6601 cagaacgatc ggctgtttgc cactggcatc aacgacggtg agatcggtac caggattcac
     6661 ggtagccagc tcgcctatgc gagaagtttt gaaaggataa atatgtgcgc cattagtcac
     6721 tgaagtagcg gcctgacttt ccagctcatg cgccgcgttg tcatccattt ttatttctgg
     6781 cggaaaatgg gcaatgcctt tatgacaatc gatacaggtt tcgccgccct tttgtgcttt
     6841 gttatgcatt ttctgcgcag attcactttg cgaggcaata tccatggcat caaaagaatg
     6901 gcaactacgg cacgttgcag agtcagttgc ttttaattct ttccatactg tttcggccat
     6961 ttcctggcga tgagtttcga acttatcgtc actgtctatt ttgccgctaa caaattcatg
     7021 ataaatatct ttagatgctt ttaatttagc aaataaataa tccatccctg actttggaat
     7081 atggcaatct gcacattccg cacgtatccc tttctggttc gaaaagtgga cagttccctg
     7141 atattcctca aaaggtttac tcatcgagtg gcaggaaagg caaaatgctg tatccgacgt
     7201 tttatgtaag gctttttgcg ccagcaataa cccgccgcca ccaaccacaa ccgctatcag
     7261 caaaaacaat aacccaatgc gttttttccc tcgcatagat tcctctaaat aaattccaga
     7321 acaccgaaat cattgtataa ttaaatacac aacgaattaa ccgcttgcat aactcggcac
     7381 aacactgcct gaaataatcg ataaagaata tgatttatta caatgtaatc attaattgct
     7441 aaggaataac ccagttgcta tttagaatat atatttatca gttttagcaa tttaaatccc
     7501 ataatcaatg tgaatataaa aaatattatt agcacaacgc tatgttcact gtcacccgct
     7561 caacagatca cggttatcgt tcgtttttta tactgctcag ggataaaaaa aggcccctgt
     7621 tgaaattgca ggggcctggt acgagcaagc atcatattgg gcgacatgat gcaacggtaa
     7681 aaatcatttg gcctgatggc gttcaatgat tcctttcatt tcagcaaccg ccgccccgtc
     7741 tacgatataa cgcgaatact cgtccgcgtg ttcatctgat gacatggaca atccttgatt
     7801 acgataacgc atcggtgagg cttgccacgc tcccgcggag ctatggactt ccagcactcc
     7861 ggcatcgagg aagtggtgca ggttttctgc acggactcct gctccggcca taatgattgg
     7921 agcatcacga tgggcaataa gttccataat ttttgataaa ccttgcagtg cgtctgattt
     7981 ttgccctgat gtcagtaccc gggcaatgcc caattccgca agattattga gtgtatataa
     8041 agggttagcg cacatatcga aggcgcgatg aaaagtcact gccagcggac cggcagcagc
     8101 cattattttt tccattcgtg gcatatcgac attcccgtca acatcgagaa cgcccgtcac
     8161 cagtccagga aaacctaatt cgcggaccgt gcgcacatcc tcaagaatgg cggcaaactc
     8221 accgtcgctg tagcaaaaat caccaccgcg tgggcgaatt atcggatgca cagggatcgt
     8281 cacccgctgg cgcacggatt tcagtacccc caacgacggc gttaagcccc cctcttttgg
     8341 ggctgcgcat aattcaactc tgtctgcgcc gttttgctgc gccgttagtg cacattccat
     8401 gctgtaacag caaatttcca gtaatgccat ttttgctcct taattacgct gactgctcgc
     8461 tggcaacgat ctcttcaatg gaccacggat gaaacttaat agttgttttg ccatcggtga
     8521 ccgccagcgt cggattcggt aatcgctcat gctcaccttt tggtgaactg gttttaactg
     8581 agatccccgg taggctgagt ccttcatcag aaagcaatgc cagcgcacgg gcattcagcg
     8641 tttctggatc acccagcaga acgatttcaa tatgttccca accttcgtgt gggtaacgtt
     8701 tttccccggg ccacggtagc tccacaatag aaaactgcca gtgcgcaacc tgtaccggtt
     8761 catgcaattt aaacagacaa atcggtctgc cattgatcat attttctgac aaaagctcgc
     8821 cacactgttc aaacccgcga cgccagcgtt cagcagtggc gttttgatgg caacgcaaag
     8881 aaatgtgatc ggcagtcagc ggagtgatat tcaaccccag acggagggaa agttcatcta
     8941 atgcgtgggt aaatcgcggt aaatccgatg caatatcctg cagctcgtcg atagattgcc
     9001 agttcgccat aatcactctt cgtctttcag taaaagcgtt aatttaccct gttgccctgc
     9061 gccaaccaac cgctgatttc acgccgcttc tgatgcaata gtgaaaacgg caatacgcca
     9121 cgcgcacgtt gctgacgaaa acagccattt gcagtatact cccgccctaa tttctttaac
     9181 tggtgcgggc aatttttgct cgcttcatca atgtaaggta ttccggtgaa tattcaggct
     9241 cttctctcag aaaaagtccg tcaggccatg attgcggcag gcgcgcctgc ggattgcgaa
     9301 ccgcaggttc gtcattcagc aaaagttcag ttcggcgact atcaggctaa cggcatgatg
     9361 gcagttgcta aaaaactggg tatgacaccg cgacaattag cagagcaggt gctgactcat
     9421 ctggatctta acggtatcgc cagcaaagtt gagatcgccg gtccaggctt tatcaacatt
     9481 ttccttgatc cggcattcct ggctgaacat gttcagcagg cgctggcgtc cgatcgtctc
     9541 ggtgtttcta cgccagaaaa acagaccatt gtggttgact actctgcgcc aaacgtggcg
     9601 aaagagatgc atgtcggtca cctgcgctct accattattg gtgacgcagc agtgcgtact
     9661 ctggagttcc tcggtcacaa agtgattcgc gcaaaccacg tcggcgactg gggcactcag
     9721 ttcggtatgc tgattgcatg gctggaaaag cagcagcagg aaaacgccgg tgatgaaatg
     9781 gagctggctg accttgaagg tttctaccgc gatgcgaaaa agcattacga cgaagatgaa
     9841 gagtttgccg agcgtgcacg taactacgtg gtaaaactgc aaagcggtga cgaatatttc
     9901 cgcgagatgt ggcgcaaact ggtcgacatc accatgacgc agaaccagat cacctacgat
     9961 cgtctcaacg tgacgctgac ccgtgatgac gtgatgggcg aaagcctcta caacccaatg
    10021 ctgccaggaa ttgtggcgga tctcaaagcc aaaggtctgg cagtagaaag cgaaggggcg
    10081 accgtcgtat tccttgatga gtttaaaaac aaggaaggcg aaccgatggg cgtgatcatt
    10141 cagaagaaag atggcggcta tctctacacc accactgata tcgcctgtgc gaaatatcgt
    10201 tatgaaacac tgcatgccga tcgcgtgctg tattacatcg actcccgtca gcatcaacac
    10261 ctgatgcagg catgggcgat cgtccgtaaa gcaggctatg taccggaatc cgtaccgctg
    10321 gaacaccaca tgttcggcat gatgctgggt aaagacggca aaccgttcaa aacccgcgcg
    10381 ggtggtacag tgaaactggc cgatctgctg gatgaagccc tggaacgtgc acgccgtctg
    10441 gtggcagaaa agaacccgga tatgccagct gacgagctgg aaaaactggc taacgcggtt
    10501 ggtattggtg cggtgaaata tgcggatctc tccaaaaacc gcaccacgga ctacatcttc
    10561 gactgggaca acatgctggc gtttgagggt aataccgcgc catacatgca gtatgcttac
    10621 acgcgtgtat tgtccgtgtt ccgtaaggca gaaattaacg aagagcaact ggctgcagct
    10681 ccggtaatca tccgtgaaga tcgtgaagcg caactggcag ctcgcctgct gcagtttgaa
    10741 gaaaccctca ccgtggttgc ccgtgaaggc acgccgcatg tgatgtgtgc ttacctgtac
    10801 gatctggctg gtctgttctc tggcttctac gagcactgcc cgatcctcag cgcagaaaac
    10861 gaagaagtgc gtaacagccg tctgaaactg gcacaactga cagcgaagac gctaaagctg
    10921 ggtctggata cgctgggtat tgagacagta gagcgtatgt aatcgatttt tcgtgagagt
    10981 aaagcctgat cagggttacc tgatcaggct tttttattgc catctaaatg tattctgaaa
    11041 atggacatgc cattgttttc tgactgttgg ataagaggcc agaagcgtaa tatccggccc
    11101 cagggaaacg ataacggttg aatttaagga ataccgcagt gtttaaattt cttgtattaa
    11161 cattaggcat tatctcttgc caggcttacg cagaagatac ggttatagaa aacgaccatg
    11221 acatttcagc catcaaagat tgttggcaaa aaaattcaga tgatgatact gacattaacg
    11281 tgatcaaatc atgcctgcga caagaataca atctcgtcga tgcgcaatta aataaagcct
    11341 atggtgaagc ttatcgttat atagaacaag tgccacgcac aggtgtaaaa aaacctgata
    11401 ccgaacaact taacttgctt aaaaaatcac agcgagcctg gctggatttt agggacaaag
    11461 aatgtgaatt aatcctttca aatgaggacg ttcaggattt aagtgaccct tattctgaat
    11521 cagaatggct ctcatgtatg atcatacaga ccaatacgcg aactcgccag ttgcagctat
    11581 accgtaactc tgaagatttt tatccaagcc ctttgacaag aggataattc acatcttttt
    11641 ggcatgtttt gttgcaagct attcctgata aataattgca acaagacatc gagccttttt
    11701 cactgagtta ttaaacatac tcgcgagcgc gtaatttttt tgtccttcag cgataattca
    11761 caatcacttc attacgttgt acctttagcg gtggaattaa ccgcccaccg cctggcactt
    11821 cccagataaa tcgcaacggt tctgctgccg ctataccgga aaaggccacg gtggttccgc
    11881 tctgcccctc taattcgaca caacgagatt gcgaacacaa gcgaacccgc agtcctgaag
    11941 gtgtcgggcc gataagctga taacgccacg ctaccagcgt catcaatcct gaagcaggtt
    12001 gtcgcgtaga aagaggcgca gacgacatcg actcaccgcg atgatttagc gtaattccta
    12061 cactacttgc ctgccacatc ccctccccgg cggcttgcac cagcagcgga aacaataata
    12121 ttgctaataa ggttctcatt atttgccgcc aattgtcgcc gtcatgcgga tatgtcggtt
    12181 atcagacagt tccagattcg acaggaccac taactgcggc aagctgcggc gcaggaagcg
    12241 agacaataat ggtcgcagcg cgtggttcac caacaatact ggcggcgcgc ccagcatctc
    12301 ctgacgggat agcgcttcct gagtttgcgc cagtaaacga tccgccagcc ctggctccag
    12361 tcctcccccg ccctgcagcg cctgtagtaa caaacgttcc agcggtgtat cgaggccaat
    12421 aacatggact tcatctttgc caggaaacca ctgctgggta atcgcccgtc ccaacgccac
    12481 gcgcacgacg gcggttaatt catgtggatc gctttggatg ggcgcatgtt ccgccagcgt
    12541 ttcgagaatg gtgcgcatat cgcgaatcgg tactttttca tcgaggagat tttgcagcac
    12601 tttatgcagt gtggtgagcg tgacgacgcc aggaacgaga tcttccgtca gctttggcat
    12661 ctcctgggcg acgcgatcca acagctgttg cgcctcctga cgaccaaaca gctctgcggc
    12721 atgctggcta atgaggtggt taagatgcgt tgctaccacc gtgctggcct caaccactgt
    12781 gtacccctga atctgcgcct gttcttttag cgcactttca atccagatag cattcaggcc
    12841 aaatgccgga tcgacggtcg cctcaccagg taacgtcccg gcagcggttc cagggttaat
    12901 cgccagccag cgccccggat aagcatcacc actgccaatc tccacgcctt tcatcaaaat
    12961 gcgatagcgg gcaggttgca gatccatatt gtcgcgaatg tgcaccactg gcggcagaaa
    13021 tcccatctcc tgggcaaatt tcttgcggat actgcgtata cggcccaaca actcaccatc
    13081 ctgctggaaa tcgaccatcg ggatcagtcg ataacccact tccattccca gagaatcttc
    13141 cagttgtaca tcgttccacg tcgcttcgac aacggtatta ttctctgcca tttttaccgg
    13201 tttgggttcg gcaggcgctt tttgttcgcg tccgcgtatc caccaggcca gcccgagcaa
    13261 tccggcagtg aacagcaaaa ataccaggtt cggcattcca ggcaccaggc cgagtaaacc
    13321 gagcacggcg gcgcttaaca acataacgct tgggttactg aaaagctgat tcaccatctg
    13381 ctcgccaaca tcctgatcgg tgctgacacg cgtaacgatg acccccgcgg cggtagaaat
    13441 caccagcgcc ggaatttgtg ccaccagacc gtcgccaatg gtcaatagcg tataactttc
    13501 cgccgcgtgt cccatgctca tgccatgttg cagcacgccg accagcaacc cgccgacaac
    13561 gttaatgacc atgatgagga tcccggcgat ggcatcgccg cgaacaaact tacttgcccc
    13621 gtccattgag ccgtaaaaat cggcttcctg agtcacttcg gagcggcgtt ttttcgcctc
    13681 atcttcacca atcaatccag cgttaaggtc ggcgtcaatc gccatctgct tacccggcat
    13741 accatcgaga acaaagcgcg cacccacttc tgcgatacgc ccggcacctt tggtaatgac
    13801 cataaagttg atgatcacga gaatgacaaa caccacgata ccgatagcga aattgccacc
    13861 aacgaggaag tgaccgaacg cttcgaccac cttccctgcc gccgccgcgc cggtatgccc
    13921 ttccatcaaa atgatacggg ttgaagccac gttaagtgcc agacgcaaca acgtagtaaa
    13981 caacagaatg gtcggaaacg cagcaaactc aagcgtgcgc tgggtaaaca tcgccaccag
    14041 caacaccatg atcgacaagg caatattgaa ggtaaacaac aggtcgagta tgaatgcggg
    14101 cagtggcagc accatcatcg acaagatcaa caggatcaaa atcggtccgg caaggatctg
    14161 ccattgtgtc gatttcaggt ttgcgggcag gcgcagcatc gcggccagat tactcatggg
    14221 tcggtttctc gttaataaaa tccagggctt ccggcaccgg aagatgagta ggttgtacag
    14281 ggcgctgtcc accagccaga cgccagcgtt tcagttgcca gacccaggcc agcacttccg
    14341 ccaccgcggc gtacagttga cccgggattt gttgaccaat ctccgcatgt cgatacagcg
    14401 ctcgcgccag cggcggcgct tcaagcgtcg ggacgttatt ttcagcgcca atttcacgaa
    14461 tgcgcagcgc gaccagccct gcacctttag cgaccacttt cggtgcgctc attttgtttt
    14521 cgtcatactg caacgctacc gaatagtggg tcgggttatt gacaatgaca tcagctttcg
    14581 gcacatcggc catcatccga cgccgtgcag cagctcgctg catctgacgg atccgccctt
    14641 taacatgagg gtcaccttcg ctttgtttga actcatcacg aatatcctgc cgcgacatac
    14701 gcagcttttt caggtggctg aagatttgga aaaagacgtc aaatcccacc attggaatga
    14761 caccaagcac caccagcagt gcgcatagcc caaccagatc catcgcatta cccatggcgg
    14821 tagtcggaga ctcggccatt aagcgcatca tctgcggcca gtgatgccag agataaaacc
    14881 ccgtcacgct gccaaccagg atggttttca aaattgcctt aagcaactcc gcgccagtct
    14941 gtgccgagaa catccgttta atgcccggta gcgggttgag tttggaaaac ttcggctgca
    15001 aggatttgcc gctaaatacc agccccccca gcatgaccgg agaaatgagc gccaccagca
    15061 ccacgccgct aatcagcggc agcagcgcca gcatggcttc tctgatcagc agaataatct
    15121 gcccgaggat cagattcggg tca
//
LOCUS       DAZPDU010000105        15030 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZPDU010000105 DAZPDU010000000
VERSION     DAZPDU010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15030
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.106"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(54..2006)
                     /gene="betT"
                     /locus_tag="ACHURS_002864"
                     /pseudo
     CDS             complement(54..2006)
                     /gene="betT"
                     /locus_tag="ACHURS_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
     gene            2145..2732
                     /gene="betI"
                     /locus_tag="ACHURS_002865"
     CDS             2145..2732
                     /gene="betI"
                     /locus_tag="ACHURS_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HFR1238541.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVAGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPREQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            2746..4218
                     /gene="betB"
                     /locus_tag="ACHURS_002866"
     CDS             2746..4218
                     /gene="betB"
                     /locus_tag="ACHURS_002866"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HFR1238542.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCRDEMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            4232..5902
                     /gene="betA"
                     /locus_tag="ACHURS_002867"
     CDS             4232..5902
                     /gene="betA"
                     /locus_tag="ACHURS_002867"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HFR1238543.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPTLDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVANGMPVRAKK"
     gene            6115..6783
                     /gene="ykgH"
                     /locus_tag="ACHURS_002868"
     CDS             6115..6783
                     /gene="ykgH"
                     /locus_tag="ACHURS_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgH"
                     /protein_id="HFR1238544.1"
                     /translation="MSEQIKQDIDLIEILFYLKKKIRVILFIIAICMAMVLLFLYVNK
                     DNIKVSYSLKINQTTPGILVNCDSNNNFACQTTMTEDVIQRITTFFHTSPDVKNREIK
                     LEWSGDKRDLPTAEAEISRVQASIIKWYASEYHNGRQVLDEIQIPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(7026..7721)
                     /locus_tag="ACHURS_002869"
     CDS             complement(7026..7721)
                     /locus_tag="ACHURS_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFR1238545.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAVERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(7714..9141)
                     /locus_tag="ACHURS_002870"
     CDS             complement(7714..9141)
                     /locus_tag="ACHURS_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFR1238546.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(9152..9871)
                     /locus_tag="ACHURS_002871"
     CDS             complement(9152..9871)
                     /locus_tag="ACHURS_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFR1238547.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(10399..11253)
                     /gene="rclR"
                     /locus_tag="ACHURS_002872"
     CDS             complement(10399..11253)
                     /gene="rclR"
                     /locus_tag="ACHURS_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFR1238548.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLQHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            11479..12804
                     /gene="rclA"
                     /locus_tag="ACHURS_002873"
     CDS             11479..12804
                     /gene="rclA"
                     /locus_tag="ACHURS_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFR1238549.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            12913..13149
                     /gene="rclB"
                     /locus_tag="ACHURS_002874"
     CDS             12913..13149
                     /gene="rclB"
                     /locus_tag="ACHURS_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFR1238550.1"
                     /translation="MFKKSVLFATLLSGVMEFSTNADDKIILKHISVSSVSASPTVLE
                     DTIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            13161..13754
                     /gene="rclC"
                     /locus_tag="ACHURS_002875"
     CDS             13161..13754
                     /gene="rclC"
                     /locus_tag="ACHURS_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFR1238551.1"
                     /translation="MEKYLHLLSRGDKIGLSLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAYTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(13914..14783)
                     /locus_tag="ACHURS_002876"
     CDS             complement(13914..14783)
                     /locus_tag="ACHURS_002876"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFR1238552.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWGQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSVWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
BASE COUNT         3938 a         3828 c         3594 g         3670 t
ORIGIN      
        1 gtcaatgatt cccgcgccat atgcagacat ggcgcggttt tatgcaaaaa caatcacgcg
       61 tccgggaaca tcacgctatg gcccggcgct tcacgatgga gatgaataaa gttaaggtgc
      121 cgctcgtact ggtcaagaat atcggtgatc acctgctctt tgctgtagtc catcaggtcg
      181 ttgccctggc tgccttctaa caggaaggtt tccagtcggt agtaggtcga tttaccgata
      241 cgtgcgcggt aggtaaagcc cggcaccgaa tattgctgcg gccaaatctg atagacaaag
      301 ttttgctctt cgcccatatg caccaacaaa tccagatgcc ccaactgttg tccctcttcc
      361 ggcggcaggc tttttagctc cacgtacgcg ccgcgcaacc gcagctcctg cgccacttct
      421 tccattgccg ggtaacagac cgtctccatc atctgtttag tgtaacgcgt gcccggataa
      481 ttcatcaggc gcgatagacg ttttttccag ctcaggcgat cctgaagccc cagcggtcgc
      541 ggcgcggtat cgcggttgac actttcacgg cggtaatctt ctaccttcag agatttatac
      601 aaccccgcca tcacgaagaa aatcacaaag ctgaacggca gccccatgat taccgtggtg
      661 ttttgcagcg cggatatccc gttggtcatc agcatgccga gcgtcagcag accaatcgcc
      721 accgaccaga agacgcgcag ccagccgggg gcgtcgctgt tgatatcttt aagctgcgag
      781 gtgaaattcc ccagcaccag cgcaccggag tccgccgagg tcacataaaa cagcaggcca
      841 gtaatggtgg cgacggaggc gctaaaggta aacgccggat actgcgccag caggctgtag
      901 aagccgcgct ccggatggac catcgcttcc tcggcaaatg ccgcgccgcc gtggatgatt
      961 tcatacagcg cgctattgcc gaataccgag agccataaca gcgtgaaggt aaacggaata
     1021 atcaacgtgc ccagcacgaa ctggcgaatg gtacgcccac gcgagatacg cgccaggaac
     1081 aagccgacaa acggcgacca tgccacccac catgcccaga agaagagcgt ccagttattc
     1141 atccactcaa ccggacggtc gaaggcaaaa ctgttgagcg tcatgcccat aaagcgattc
     1201 acatagtcgc caacgttcag caccagcgca ttaagcagga acgaggtgtc gcccataaac
     1261 aatacgaaca ggatcaatcc cagcgccagc gcaacgttaa gttccgataa cacacgaatg
     1321 cccttatcga caccggaggt gacagagatc gtggcgatta tcaccgacaa ggcgatcagt
     1381 gccgcttttg ccgccatcga atcgggaata tcaaacagta cgctcaagcc atagttaagc
     1441 tgcaccacac cgataccgag cgtagtggca ataccgaaga tagtgccgat caccgctgca
     1501 atatccactg agtgacctat cggcccgtta atccgtttac cgaagatcgg gtacagcgcc
     1561 gagcggatgg tgagcggcaa attataacga tagctaaagt atccgagcgc catgcccatc
     1621 agcgcataca tcgaccagcc ggttaagccg tagtgaaaca gcgtccagac catcgcctga
     1681 cgcgcggcct caatcgtctg tcccgcgcct tccggcggct gcatatactg cgttaccggt
     1741 tcggctacgg agaagaacat caggtcgata ccgatcccgc cagcaaacag catcgccgcc
     1801 caactcagca ggctgaattc cggtttggat tgttctggcc cgagcttcac cgaaccaaaa
     1861 cgcgaacaag cgatacagac cacaaagaca atatagagcg ttgccgccag tagatagtac
     1921 caaccgaagg ttttagaaac ccagtccagc gtgcggccaa tccacagggc cgagaagtcg
     1981 cgaaacagga tcgttgtcag ggaaaaggtc tgtcatccag tatccccact gttattgtta
     2041 ccatttaaaa tcaaattcgt aacaattacg acacatttta tattgaacgt ccaatcaata
     2101 accgctttaa tagataaaca acgctgatga atggagtggc gaaaatgccc aaattgggga
     2161 tgcagtcgat ccggcgcaga caactgatcg acgccacact ggaagcaata aatgaagtgg
     2221 gcatgcacga tgcaacgatc gcgcagatcg cccgccgtgc aggcgtttct acggggatca
     2281 tcagccacta tttcagggac aaaaatggtc tgctggaagc aaccatgcgc gatatcacca
     2341 gtcagctgcg tgacgcggtt ttgaatcgat tacatgcact tccgcagggc agtgcagagc
     2401 agcgattaca ggcgattgtt gccggaaact tcgatgaaac tcaggtgagc agtgcggcga
     2461 tgaaagcctg gctggcgttc tgggccagca gtatgcatca gccgatgctc tatcgtttac
     2521 agcaggtcag cagccgccgc ttgctgtcga atctggtgag cgagtttcgc cgcgaattgc
     2581 cgcgcgaaca ggcacaggaa gcgggctacg gccttgccgc gctgattgac ggattatggc
     2641 tgcgcgcggc actaagcggc aaaccgctgg ataaacctct cgcacactcg ctgacccgtc
     2701 actttattac tcagcattta cccaccgatt aaccgaggag acgtgatgtc ccgaatggca
     2761 gaacagcagc tttatataca tggtggttat acctccgcca ccagcggtcg caccttcgag
     2821 accattaacc cggccaacgg taacgtgctg gcgaccgtgc aggccgccgg gcgtgaggat
     2881 gtcgatcgcg ccgtgaagag tgcccaacag gggcaaaaaa tctgggcggc gatgaccgct
     2941 atggagcgct cgcgtattct gcgtcgggcc gttgatattc tgcgtgaacg caatgacgaa
     3001 ctcgcaaaac tggaaaccct cgataccgga aaagcatatt cggaaacctc aaccgtcgat
     3061 atcgttaccg gtgcggacgt gctggagtac tacgccgggc tgatcccggc gctggaaggc
     3121 agccagatcc cgttgcgtga gacgtcattt gtttataccc gccgcgaacc gctgggtgtg
     3181 gtggcgggga ttggcgcatg gaactacccg attcagattg ccctgtggaa atccgccccg
     3241 gcgctggcgg caggcaacgc aatgattttc aaaccgagcg aagtcacccc gcttaccgcg
     3301 ttaaagctgg ctgaaattta cagcgaagcg ggcctgccgg acggcgtatt taacgtgttg
     3361 ccgggggtgg gcgcggagac cgggcaatat ctgaccgagc atccgggcat tgccaaagtg
     3421 tcatttaccg gcggtgtcgc cagcggcaaa aaagtgatgg ctaactcggc ggcctcttcc
     3481 ctgaaagaag tgaccatgga actgggcggt aaatcaccgc tgatcgtttt cgatgatgcg
     3541 gatctcgatc tcgccgccga tatcgccatg atggcaaact tcttcagctc cggtcaggtg
     3601 tgtaccaatg gcacccgcgt cttcgttccg gcgaaatgca aagccgcatt tgagcagaaa
     3661 attctggcgc gcgttgagcg cattcgcgcg ggcgacgttt tcgatccgca aactaacttc
     3721 ggcccgctgg tcagcttccc gcatcgcgat aacgtgctgc gctatatcgc caaaggcaaa
     3781 gaggaaggcg cgcgcgtact gtgcggcggc gatgtactga aaggcgatgg cttcgataac
     3841 ggcgcatggg ttgcaccgac cgtgttcacc gattgccgcg acgaaatgac catcgtgcgc
     3901 gaagagatct tcggtccggt gatgtcgctt ctcacttatg aatctgagga cgaagtgatt
     3961 cgccgcgcca atgataccga ctacggcctg gcggcgggca tcgtgacggc ggacctgaac
     4021 cgcgcgcatc gcgtcattca tcagctggaa gcgggtattt gctggatcaa cacctggggc
     4081 gaatccccgg cagagatgcc cgttggcggc tacaaacact ccggcattgg tcgcgagaac
     4141 ggcgtgatga cgctccagag ttacacccag gtgaagtcca tccaggttga gatggctaaa
     4201 ttccagtcca tattctaacc aggaggttta tttgcaattt gactacatca ttattggtgc
     4261 cggctcagcc ggcaacgttc tcgctacccg tctgactgaa gatccgaata cctccgtgct
     4321 gctgcttgaa gcgggcggcc cggactatcg ctttgacttc cgcacccaga tgcccgcagc
     4381 gctggcgttc ccactacagg gcaaacgcta caactgggcc tatgaaacag agcctgaacc
     4441 gtttatgaat aaccgccgca tggagtgcgg acgcggtaaa ggcctgggtg gatcgtcgct
     4501 gatcaacggc atgtgctaca tccgtggcaa tgcgctggat ctcgataact gggcgcaaga
     4561 acccggtctg gagaactgga gttatctcga ctgcctgccc tactaccgca aggccgagac
     4621 tcgcgatgtg ggcgagaacg actaccacgg tggcgatggc ccggtgagcg tcactacctc
     4681 caaacccggc gtcaatccgc tgtttgaagc gatgattgaa gcgggcgtgc aggcgggcta
     4741 cccgcgcacg gacgatctca acggttatca gcaggaaggt tttggtccga tggatcgcac
     4801 cgtcacgccg cagggccgtc gcgccagtac cgcgcgcggt tatctcgatc aggccaaatc
     4861 gcgccctaac ctgaccattc gtactcacgc tatgaccgat cacatcattt ttgacggcaa
     4921 acgcgcggtg ggcgtcgaat ggctggaagg cgacagcacc atcccaaccc gcgcaacggc
     4981 caacaaagaa gtgctgttat gtgcaggcgc gattgcctca ccgcagatcc tgcaacgctc
     5041 cggcgtcggc aacgctgaac tgctggcgga gtttgatatt ccgctggtgc atgaattacc
     5101 cggcgtcggc gaaaatcttc aggatcatct ggagatgtat ctgcaatatg agtgcaaaga
     5161 accggtttcc ctctaccctg ccctgcagtg gtggaaccag ccgaaaatcg gtgcggagtg
     5221 gctgtttggc ggcactggcg ttggtgccag caaccacttt gaagcaggcg gatttattcg
     5281 cagccgagag gaatttgcgt ggccgaatat tcagtaccat ttcctgccag tagcgattaa
     5341 ctataacggc tcgaatgcgg tgaaggagca cggtttccag tgccacgtcg gctcaatgcg
     5401 ctcgccaagc cgtgggcatg tgcggattaa atcccgcgac ccgcaccagc atccggcaat
     5461 tctgtttaac tacatgtcac acgagcagga ctggcaggag ttccgcgacg caattcgcat
     5521 cacccgggag atcatgcatc aaccgacgct ggatcagtat cgtggccgcg aaatcagccc
     5581 cggcacggaa tgtcagacgg atgagcagct cgatgagttc gtgcgtaatc acgccgaaac
     5641 cgccttccat ccgtgcggta cctgcaaaat gggttacgac gagatgtccg tggttgacgg
     5701 cgaaggccgc gtgcatgggc tggaaggcct gcgcgtggtg gatgcgtcaa ttatgccgca
     5761 gattatcacc gggaatttga acgccacgac aattatgatt ggcgagaaaa tagcggatat
     5821 gattcgtgga caggaagcgc tgccgaggag cacggcggga tattttgtgg caaatgggat
     5881 gccggtgaga gcgaaaaaat gagtcgtgat gtgaactaac gcaggaaccg atcagattga
     5941 gagttaccgt tccagagagg gggaccgaat ccttatataa acactgaggt aactctcatg
     6001 cttcatatcc agtattcatg atgctggttt tttgctgata tttaccccga acattcctga
     6061 tacagactat taatacaacc ttatctgcgg cagattttat tttctggata tattatgagt
     6121 gaacaaatca aacaggatat cgatctgatt gagattttat tttatctgaa gaaaaagatt
     6181 cgtgttatcc tttttattat agctatctgt atggcaatgg tgcttttatt tctgtatgtc
     6241 aataaagata atataaaagt gagttacagc ctaaaaataa accagacaac accaggcata
     6301 cttgttaact gtgatagcaa taataacttt gcctgtcaga ccacaatgac tgaagatgtt
     6361 atacagcgaa ttactacatt ttttcacacc agcccagatg tcaagaacag agaaataaag
     6421 ctggaatggt caggagataa gagagattta ccaactgctg aagcggaaat atctcgcgtg
     6481 caggcgtcta ttatcaaatg gtatgcgtca gaataccata atggcaggca agttctcgat
     6541 gagatacaaa taccttcagc aattaacagt gagctttata caaaaatgat atacctgacc
     6601 aggaactggt cattgtatcc gaacggtgat ggctgtgtaa ctataagctc accagaaata
     6661 aaaaataaat accctgctgc catttgcctg gctctgggat tttttctaag cattgtaatt
     6721 tctgtaatgt tttgccttgt caaaaaaatg gtagatgaat accaacaaaa ctctgggcag
     6781 taataaacag cattctgata gagagtgcct gtataaagct gaagtgacaa tgaccgggaa
     6841 gggaacccgt tttcaggaat ggatgcctgt ctttttcact gtaaatatcc tggcattact
     6901 aatgccagga tatttacaga agagcagcat aaccatgacg gaacggggtt aagtcaggtt
     6961 gtccttaacg ctattttcaa cctcgtatgc cttcttcagg tttatgttca gatctcatat
     7021 ctctctcagc aatcctcaat aatcagatac accgctttca ccggaccatg aaccccgacg
     7081 actttgataa gctcaatatc cgccgttgag ctgggaccac tgatgatgtt aatacatgac
     7141 ggcattcgtt caccggcctg cgctttctga tgcaattttt ctgcgagttg cgctacacgc
     7201 ggcaggattg tgcttttacg caggataaaa agagaagatt ccgggagcag gctcaatgaa
     7261 cgcccgcgct cgacggcgga aaaaagaacc acgcctcccg attcggttaa accatattca
     7321 gcatacacga cacccacttt agcctgctct gcctgcgaga tattctcggc acctttcgcc
     7381 ggatcccaaa caacggcatt gcattcctgc tgcaaacgtt cactaatccc caattcctcc
     7441 agcctcgtgt caccgctaat cacgaccgac tgatctccca gctctttaca cagacgtatt
     7501 gcagcttctg ccgccttcgc ctcgctggtc agctcacagc gcgtcaacat aacatcgctg
     7561 gcaaactgaa taaacgcgtc acagcgctgc tgttggttaa gttgggtaag ccgctcgtta
     7621 gcatagttgt taagcggcgc atcttctgct tgcggttcaa gtcgcagcgg gcgacccagt
     7681 gcctgagcaa cgttattcaa aaattcgctc cgattatcca ttctttttct cctgcgcctg
     7741 atgtttctta aaccaactac ggaaactctc tccgtcagct tcaggaagat cgcgtgcttc
     7801 catccagtcg ctaatcgcgc caaatttgag tggtgttttg ccgccattga taaaccagct
     7861 tgccgcatga gcgccggcca tcatcccgac tttccacaat cctggatgac tattggcata
     7921 agcgaacatt tttatcgccc gttgctctgc ttttgcggtg atcccttttt cagccatcac
     7981 ccgacgatga cgcaaaatca gttttgacag cggaatacgc accggacaca cgctgtcaca
     8041 agcagtgcat aaagagcagg cgtagggtaa atctttaaaa tctttatagc cgccaagtag
     8101 cggagaaatc accgcaccaa ttggccctgg ataaatagag ccatatccat gaccgccaat
     8161 atggcgatat gccggacaag tattcataca agccccgcag cgaatacagc gcagcacatc
     8221 ccgaaattca gaggccagca cctcagaacg cccgttatcg acaataacca gatgaaactc
     8281 ttcaggacca tcaacgtgcc ccgcttcgcg cggtcctgtc agccaggtgt tgtatcccgt
     8341 caaacgtgca ccaacggcac tgcgcgccag catggtgatc aatacatcta cctcggcaaa
     8401 cgtgggggca atacgctcca ttcccatcac tgcaatatgc gttttaggca gcgtggtaca
     8461 cattcgcgca ttaccttcat tggtcactag gcataccgaa cctgtctctg ccaccgcgaa
     8521 attacagccg gtaataccta tttcagcact gaggaaatct tcgcggattt tttgccggat
     8581 gaataaggtc atcgcttcag gcgtttccgg cccctcatag cccagacgtt cgtgtagcac
     8641 tcgacggatc tgatggcgat ctttatgaat tgccgggacc acaacatgag atggcggatc
     8701 ttgatccagc tggagaatat attcacccag atcggtttca atcacctgaa tgccagcatc
     8761 ctgcaacaca tgattgacac caatctcttc ggtcaccatc gatttagatt tcaccacctt
     8821 ccgggcattt ttgcgttggg caacctgtaa aatgtagcgg gtagcgtctt ctttggtttt
     8881 tgcaaaccag acgtgaccgc cgttttgcgt cactttttct gagagctggt acagataagc
     8941 gtcgagatta ctcagaacat gatcacgtat ctgggcggcc cgatcgcgcc actcctccca
     9001 gtgccccaat tcatcgacca ttttttgccg atttgcccca atacgctgct gcgcgtttgc
     9061 caccgctttg cgcatgatcg gatcttcaat ttgctgacgg atgcgtgtct taaaatctgt
     9121 attactggtt ttgatcgaca tctttatatc ctcagcggct catcaacact tcagcaatat
     9181 gcatcacttt gactttctgc ccttcccgtt gtaatcgccc actgatgttc agcaggcaac
     9241 tcacgtcagc accaattaaa tactcagggc ggacttccat caggtgcaca accttttctt
     9301 tcaccatctc gccggatatt tcggccattt tgaccgagaa cgtgccgcca aatccgcagc
     9361 aggtatcctg ttcagcaaag gtcaacagct ccagtccacg cacatttttc agcagcgtaa
     9421 gtggctcgtc cttcactccc agcttacggg ccaggctaca agatgggtga tacacagctc
     9481 tcccttgcaa actggcacct acatcgacta cccctaattt attaacaata aaagaggtga
     9541 gatcctgcat acgcgcggca accttttcgg cacgtgatgc ccattcgggt tcatccgcca
     9601 gatacgtcgg gtaacttttt acggcatagg tgcaagagcc agccggtgaa ataatgggat
     9661 cgtcgttatc ctccagtgcg gcgatcagat ttttcatccc tggaatcgct tctttgatat
     9721 aaccgctatt gatcgcaggc tgaccgcagc atccctgttt ctccgggaaa tttacgcgac
     9781 agccgagttt ttccagtagc agcacggagt ctcgtgccat tcttgatttc agggcgtcac
     9841 caatacaggt gacaaagaaa ttgacattca caactaatac tccattactt catgcccatt
     9901 tatgcccgat ggttcccctc cttccggggg atcaatcatc aataaatatg gcgttctttt
     9961 ttaattggct agcaacatac cacccctcat ttgtgtgaca aatgataatc tttcatccaa
    10021 acttcaaatg caataactaa ttttatttct ttcggcatcg ctcacatttt atggggaaat
    10081 cgacgttcat ttttggcata agaaatccat ttaaaaaggc tacatccctt atacctattc
    10141 agagctactt attcaggggt tattgagata tacactgatg gtgtacaaaa aaatcaccga
    10201 caataatcac gtatcaagta atagtcgggg ttttatttaa ttgaagcaat ttagtttgca
    10261 tttttattta aaaactgagc tagagtccat cagaaaaaaa tatataaagc ccaaaccacc
    10321 gttggaaata gaaatagttg aaaggctcac aactcccgga atgcggatta tctttgccct
    10381 gatttctggc ggttttattc agggtgcaag ctgtctgacc ctttcccgat attctcccgg
    10441 agtacaacca aactcgcgga caaacgcctt gtgaaaagat gattcactgg catagcctac
    10501 tgactcagcg atcaccacaa cagggagcat ttcccgggaa aacatctggg ccgctatttg
    10561 tagacgcaac tttgttaata cagccagcgg cgtggttccg gaaacatcac ggaaaagctg
    10621 ggcaaaactt gcccgagaca tgtgggcgat gctggccagc gattcgacgg tccaggcgtg
    10681 tcctggcatt tccagcattt gctgtattac cgcaccaaga cgtggatgca gaagcaaact
    10741 gagaatgttt ttctctgtat taacctgtgc aatccactca cgcaccgcga gggtaaagaa
    10801 tgtagcgcag atctggctac acagtgcatc cactcccggc atcgccgatc tggattcctg
    10861 ttgtaaaaac gggatcgcct ccctcagcca gttatattcc acgctgtggt taaccggtgc
    10921 taaaaacagc gtttccggca aagacgttaa aaaataacgc gccgaatgtt gcaaccgaag
    10981 agtgccgcag acaatacagg tcgattcatt atcgacatga ctcaggcgat gagctgaatt
    11041 ttgtggtagc aggaccacat ttcccgggcg taatgtaaaa atctctcccg tcggcatttc
    11101 cagcttcgcc gctccttgcg ttaacgcatg ccaacgaata accgataatt ccccggcacc
    11161 atgttgaagc tgccagtcac ttcctaacac gcaattctta tcgatcgttc cttgtggagc
    11221 gttaagcatc aacaaacggc taagggcatc catatcagac tcctgagtgg tttttgatta
    11281 caaacaccaa caatcttagt aaataactaa tctaatacca tcaaatggtt aatcatgatt
    11341 tttgttttgt gattctgatt aagaaaaaga aaataccaga cgataagaca aaatatcaag
    11401 cgttgtgaag aaatggtatt tgctcttttg cgtctatagt catgatgtca aatgaacgtg
    11461 tttcgacagg aaatcatcat gaataaatat caggcagtta ttattggttt tggcaaggct
    11521 ggaaaaacat tagccgtcac gctggcaaaa gcaggttggc gagtggctct catcgaacaa
    11581 tcaaatgcaa tgtatggcgg gacctgtatt aatatcggct gcatcccaac caaaacattg
    11641 gttcatgacg cacagcagca cacagatttt gtccgtgcca tacagcgtaa aaatgaagtg
    11701 gttaattttt tacgtaataa gaattttcat aatcttgcgg atatgcccaa tatcgacgtg
    11761 atcgacggcc aggcggagtt tatcaataat catagcctgc gtgttcatcg gcctgaggga
    11821 aatctggaaa ttcatggcga gaaaattttt attaataccg gtgcacaaac cgtggttccg
    11881 ccaattcctg gaattaccac cacgcctggt gtatatgaca gcaccggatt acttaatcta
    11941 aaagaattgc ctgggcattt aggtattttg ggcggcggat atattggcgt tgagttcgcc
    12001 tctatgttcg ctaattttgg cagcaaggta accattttag aagcagcttc gctgtttttg
    12061 cctcgggaag atcgggatat tgctgataat atcgcgacga ttttacgcga tcagggcgtc
    12121 gatattatcc tcaatgccca tgtggagcga atcagtcacc atgaaaatca agtgcaagtg
    12181 catagcgagc acgcccaact ggcggtggat gcactgttaa tagcttccgg tcgtcaaccg
    12241 gctaccgctt cgttacatcc agaaaatgcc ggtatcgcag taaacgagcg cggggcaatt
    12301 gtcgttgaca agcaattaca taccaccgca gacaatattt gggcgatggg agatgttacc
    12361 ggcgggctgc aatttactta tatttcactg gatgattacc gcattgtgcg tgatgagttg
    12421 ctgggagaag gcagacgtag tactgatgat cggaaaaatg tgccttattc cgtattcatg
    12481 acaccgccac tgtccagggt tggtatgaca gaagaacagg ccagagagag tggtgctgat
    12541 attcaggtgg tgacattgcc tgtagctgca attccgcgtg ccagagtgat gaatgatact
    12601 cgtggggtat taaaagcgat tgttgataat aaaacccaac gtatattagg ggcatcactg
    12661 ctgtgtgttg actcccacga gatgatcaat atagtgaaaa tggtgatgga tgccgggctg
    12721 ccttatagca tattacgcga tcagatattt actcatccgt cgatgagcga atcactcaat
    12781 gatctatttt cattagtcaa ataaactcaa aatcagacgc cagaacaaat attctggcgt
    12841 ctcagagaaa agaatcttat taattctctg ttactctata acttcttaat cacttcattg
    12901 atggtatttt atatgtttaa aaaatctgtt ttatttgcaa cactattatc tggcgttatg
    12961 gaattttcca ccaatgcaga tgataaaata attctgaaac atatcagcgt ctcgtcagta
    13021 tcagcatcac cgacagttct ggaggatacc attgctgata tagccagaaa atataatgct
    13081 tcatcctgga aagtcacatc gatgcgaatt gataataatt caaccgcaac agcagtattg
    13141 tataaataag gatgttcaca atggaaaaat acctgcacct gttaagtcgg ggagataaaa
    13201 ttggcctgtc attgattcgt ctgagtattg cgattgtttt tatgtggatt gggttattaa
    13261 agtttgtccc ttacgaggca gacagcatta caccattcgt cgcaaacagt ccactaatgt
    13321 cgttctttta tgaacacccg gaagactata aacagtatct gactcacgaa ggcgaataca
    13381 aaccagaagc aagggcatgg caatcggcca ataataccta tggtttttcc aacggtcttg
    13441 gcgtcgtgga ggtgattatt gctctgctgg ttttggctaa tcctgtcaat cgctggttag
    13501 gtttattggg aggactgatg gcatatacca cgccgctggt aacactctca tttttaatca
    13561 ccaccccgga ggcatgggta cccgcattgg gtgacgctca tcatggtttc ccttatttat
    13621 ccggtgctgg tcgcttggta ttgaaagata ctctgatgct ggcaggtgct gtcatgataa
    13681 tggcagattc ggcgcgggaa attcttaaac aacgcagtaa tgaatccagt tcaacgttaa
    13741 aaactgaata ttgataatcc gaacactgtc tgttgttcac tttttcaggc gtcaggcccc
    13801 ctttctgatg atgaagcctg acgccatatt accggagata tgacagagtg aagatcaata
    13861 tcactcccct gttaaaagat tcttgtcgtc tcaggtaaaa tatatgatta atatcaatca
    13921 tgtattttta cagcaagaca accacgaaca aattcaggat taaaatgttt taccgactcc
    13981 ccaacataac ccaaatcaag cgaactaatt tgtgccattt ctttctctgt cagtgagaaa
    14041 tcccagatag caaaattttc ttcgatacgc tgttgatgtg tcgatttcgg aataacgacc
    14101 actccccgtt gaatattcca acgcagaatg acttgagaaa tcgatttttg atgggcatct
    14161 gcaatactct gaagcagatt attttcaaag ggtttatgtc gtccaccacc taacggagcc
    14221 catgcttcag gctgcacgtt ataatacttc atggttgcca gcgcctctgg ttgggcaaaa
    14281 tagggatgca actcgacctg gttgaccatc ggtttaactc ttaccgtttc acaaaagttc
    14341 gccagaacat gaggatagaa attggaaacc ccaattgctt ttaatttgcc ctcttcatag
    14401 gcatcttcga gtgcacgcca cacactgaaa taatcgcgca tagcctggtg caataaatag
    14461 aggtcgaagt actctagtcc cgattttttt aatgatgctt caatacctgc tgctgccgta
    14521 tcctgattca acatatcctg cccccacaat ttagacgtta taaataactc atctcgggta
    14581 cataaacctt cagatatagc ttcacgaacg gcttcaccga cggcatcttc attaccgtat
    14641 accgctgcag tgtcgatgag tcgataaccc gtgcggatag cgttcaacac tgactgtttg
    14701 cacacgtttt tatcggtaac ctgaaaaaca ccaaatccca tcatcggcat tttcagattg
    14761 ttacttaata cggaaaattc cactgtcagt ccctccatta ggcatgaaca atgagtctac
    14821 gttaaaacat aacctcaaat tagtatgtgg attttgatat cacttatgca aaaaattcat
    14881 taataatgta ggactgaaac ctctctattt tcggggacaa cgaagcagac gctaccagtg
    14941 cttttgcctt cgaccttgct gtttttgata cacttagggc cctgggcata acgaaaatgt
    15001 gcgatatcac gaagaattaa tggagaatgt
//
LOCUS       DAZPDU010000106        14997 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZPDU010000106 DAZPDU010000000
VERSION     DAZPDU010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14997
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.107"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..161
                     /locus_tag="ACHURS_002877"
     CDS             <1..161
                     /locus_tag="ACHURS_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFR1238553.1"
                     /translation="WQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSMCYR
                     AIYLRQES"
     gene            complement(199..1260)
                     /locus_tag="ACHURS_002878"
     CDS             complement(199..1260)
                     /locus_tag="ACHURS_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFR1238554.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            1473..2936
                     /gene="bepA"
                     /locus_tag="ACHURS_002879"
     CDS             1473..2936
                     /gene="bepA"
                     /locus_tag="ACHURS_002879"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFR1238555.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSQQQARYDARIDQLRQLQERFKPYTKM"
     gene            2957..3316
                     /gene="arsC"
                     /locus_tag="ACHURS_002880"
     CDS             2957..3316
                     /gene="arsC"
                     /locus_tag="ACHURS_002880"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFR1238556.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKKNGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(3454..4155)
                     /locus_tag="ACHURS_002881"
     CDS             complement(3454..4155)
                     /locus_tag="ACHURS_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFR1238557.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(4250..5539)
                     /gene="uraA"
                     /locus_tag="ACHURS_002882"
     CDS             complement(4250..5539)
                     /gene="uraA"
                     /locus_tag="ACHURS_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFR1238558.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(5625..6251)
                     /gene="upp"
                     /locus_tag="ACHURS_002883"
     CDS             complement(5625..6251)
                     /gene="upp"
                     /locus_tag="ACHURS_002883"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFR1238559.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            6576..7613
                     /gene="purM"
                     /locus_tag="ACHURS_002884"
     CDS             6576..7613
                     /gene="purM"
                     /locus_tag="ACHURS_002884"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFR1238560.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            7613..8251
                     /gene="purN"
                     /locus_tag="ACHURS_002885"
     CDS             7613..8251
                     /gene="purN"
                     /locus_tag="ACHURS_002885"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFR1238561.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTIRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            8422..10488
                     /gene="ppk1"
                     /locus_tag="ACHURS_002886"
     CDS             8422..10488
                     /gene="ppk1"
                     /locus_tag="ACHURS_002886"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFR1238562.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            10493..12034
                     /gene="ppx"
                     /locus_tag="ACHURS_002887"
     CDS             10493..12034
                     /gene="ppx"
                     /locus_tag="ACHURS_002887"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFR1238563.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(12073..14316)
                     /gene="pdeF"
                     /locus_tag="ACHURS_002888"
     CDS             complement(12073..14316)
                     /gene="pdeF"
                     /locus_tag="ACHURS_002888"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFR1238564.1"
                     /translation="MKLNATYIKMRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYMDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIVPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(14498..14650)
                     /locus_tag="ACHURS_002889"
     CDS             complement(14498..14650)
                     /locus_tag="ACHURS_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1238565.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKTSVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            14668..14859
                     /locus_tag="ACHURS_002890"
     CDS             14668..14859
                     /locus_tag="ACHURS_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFR1238566.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
BASE COUNT         3964 a         3821 c         3695 g         3517 t
ORIGIN      
        1 tctggcaact ggcgcacagt agcgcagaca attttccggc actgacggta agccatttta
       61 ttaccgccaa tctgctcccg gtgatgctgg gtaatattat cggcggtgca gtgctggtga
      121 gtatgtgtta tcgggctatt tatttacgtc aggaatcctg aaaaatcagc ccggcgaaac
      181 agttcgtcgg gctgaagatt attcttgcgc tatttgccca tcgggccagg cgtggatcac
      241 ggctttgatc agcgtcgcca atggaatagc gaagaatacg ccccagaatc cccacaaacc
      301 accgaagatc accaccgata aaataatcac cagcggatgc aggttaaccg cttcggagaa
      361 caacaccggt actaacagat taccgtccag cgcctgaata atcagataca ctgcgaagca
      421 gctccagaat tccgtgcctg caccaaactg gaatagcgcc acgccaacca ccggaatggt
      481 caccacaaat gcgccaatgt acggaataag taccgagaag ccgaccagca ccgccagcag
      541 cagcgaatag ttcagcccaa agagcaagaa ccccagccaa gtggcgatcc ccaccacgat
      601 catctccagc actttgccgc ggatatagtt ggtgatttgt tgattcatct ccttccacac
      661 ctgtcctgcc agtccacggt tgcgcggcag cacccgacga acggcgttca gcatctgctc
      721 tttgtctttc agcaggaaga agaccatcaa tggtaccagc accagataga cggcaatggt
      781 cagcaaaccg accagcgagg cgagggaaat tttcaccacc gaatcgccca tggtcagcat
      841 ccgactgcgc atattttcgg ccattgcatc aataatgccc gcgtccatta atgccggata
      901 gcggcgcggc aacgtggcgg caaagtcaga aagcttattg agcatccccg gcatatcgcg
      961 gatcaagtag atgccctgtt gccaggcgat aggcagtacc acgaacgcca tcagtagcaa
     1021 tataccgacg aaaaccacca atacaatcga cgtcgcccag cggcgggagc agccaataga
     1081 ttgcaggcgc acggttggcc attccagcaa ataggccagc acaatagcca ccagcaacgg
     1141 agcaagcagg ccactaaaga aaaagataat gccaaatccg gcaactaaaa taaccagcaa
     1201 ggcaatcgct tccgggtcgc taaaacggcg gcgataccat tgcatcaaca tttcgagcat
     1261 aaagaccttc cctgaacctc aagagcggga ttgcgatccg caattgtatc gaaatgtcac
     1321 aaaaaagact tcgcttttta tgacggattc aggaaactga aaagtcattt gagtgggcta
     1381 atcttcgccg ttacactcaa aggcggcgcg gtgggaacaa tatttcacag tatcggtcaa
     1441 atgactatct tcagaaatac aggatagagg ttatgttcag gcagttgaaa aaaaacctgg
     1501 ttgcaaccct cattgctgct atgaccattg gtcaggtagc cccggcgttt gccgacagcg
     1561 cagacacctt gccggatatg ggaacctccg caggaagcac gctttccatt ggtcaggaaa
     1621 tgcagatggg cgactattat gtccgccagc tacgcggcag cgcgccgtta attaatgacc
     1681 cgctgttaac gcaatatatt aattcgctgg ggatgcgtct ggtttcgcat gccaattcgg
     1741 ttaagacacc gtttcatttt tttctgatca acaacgacga aattaacgcc tttgctttct
     1801 ttggcggcaa cgtggtgctg cactctgccc tgttccgtta ttccgataac gaaagtcaac
     1861 tggcttcagt tatggcgcac gaaatctccc acgtcaccca acgtcacctg gcgcgagcga
     1921 tggaagatca gcagcgcagc gcgccgctga cctgggtcgg cgcgttaggt tctattttac
     1981 tggcgatggc cagtccgcag gcggggatgg cggcgctgac cggtacactg gcgggaacgc
     2041 gtcaggggat gatcagtttc acccagcaaa atgaacagga agcggaccgc attggtattc
     2101 aggtgctgca acgctcggga ttcgatccgc aggcgatgcc aaccttcctc gaaaaattac
     2161 tcgatcaggc gcgttactcc tcgcgcccgc cggaaatttt attgactcac ccgttgccgg
     2221 aaagtcgtct ggcagatgcc cgcaaccgtg ctaatcagat gcgcccgatg gtggtgcagt
     2281 cgtcggaaga tttctatctg gcgaaagcgc gcacactggg gatgtataat tccggacgta
     2341 accagctcac cagtgatttg ctggatgaat gggcgaaagg aaacgttcgt cagcaacgag
     2401 cggcgcaata tggtcgtgct ttacaggcga tggaagccaa taaatacgac gaggcgcgta
     2461 aaacgctgca accgttactg gcggcagaac ctggcaacgc atggtatctc gatctggcta
     2521 ccgatatcga tcttgggcaa aacaaagcca atgaggcaat caatcgcctg aaaaatgccc
     2581 gtgatttgcg caccaatccg gtgttgcagc tcaacctggc aaacgcttat ctgcaaggcg
     2641 gtcaaccaca agaagcggcc aatattctga atcgctacac ctttaataat aaagatgaca
     2701 gcaacggctg ggatttactg gcacaggcgg aagccgcgct aaataaccgc gatcaggagc
     2761 tggctgcgcg agcagaaggt tatgcgctcg ccggacgact cgatcaggcc atttcgctgt
     2821 tgagtagcgc cagttcgcag gtgaaattag gcagccagca acaagcgcgt tacgatgcgc
     2881 gcatcgacca gttgcgccag ctgcaggaac gctttaagcc ttataccaag atgtaattcg
     2941 cattaaagga agaacaatga ccaaacaggt aaaaatctac cataacccac gctgttcaaa
     3001 gagccgggaa acgctgaacc tgctgaaaaa aaacggcgtg gagccagaag tggtactcta
     3061 ccttgagaca cccgccgatg cggcaacgct gcgcgatttg ctgaaaatgc tggggatgaa
     3121 tagcgcccgt gaattgatgc gccagaaaga ggatctttat aaagaactga acctggctga
     3181 cagttcgctt agcgaagagg cgctgattca ggcaatggtg gataatccga agctgatgga
     3241 gcgcccgatt gtggtcgcga atggcaaagc gcggattggt cgtccaccgg agcaggtact
     3301 ggaaatcgtg ggttaagatg tgccccatcg ctagttgaag cacattgccg gatgcgacgc
     3361 ttgccatgtc ttatccggcc tacgaatact atggattaac ttcgcgtagt tcggataagg
     3421 cgttcgcgcc gcatccgaca ataaacacct tatctacaac ttcagaattt ctttcacaaa
     3481 cggaatggtc agcttacgtt gcgcggtaat cgacgcacga tccaactgat ccaacgtcat
     3541 aaatagcgtg cgcatttctc tgtcgagccg cttcagcaag aaacgcccca catcttccgg
     3601 cagttcaaaa ccacgcaaac gcgcgcgtaa ctgtagcgcc tgcaacttat cttcatcaga
     3661 aagtggctgc aatttgtaga tctgccccca gtcgagtcgc gacgcgagat ccggtaatcc
     3721 cagattcaac tgccgcggtg gacgatcgcc ggtgatcaac aatcgtgttt tgcccgattc
     3781 cagaattcga ttgtagagat cgaaaatcgc catctcccac aactcatcgc ctgcaataca
     3841 ctcaatgttg tcgatacaga ccagcgacaa atgctccata ccgtcgagca cttccggaac
     3901 aaaccaggtg cgtttatcca gcgggacata gcccaccgca tcgccacgct gcgacaattc
     3961 cgcgcaagcc gcgtgcagca gatggctgcg ccccgcgcct tcgcgtgccc agagatagat
     4021 gtaaccgcta tgttcctgac gcagcacgtt ttgcagcgca gccagtaaag aggagttatc
     4081 ccccggccag aaacttgcaa aggtttcgtc gtcaggaaga taaagtggca aagagagctg
     4141 tgccggtgtg ttcagagata cctcaaccag gatttcacaa aatcgcgaga agtttaccac
     4201 agaatcccat gatgtttgaa ccgggcagca acactgcccg gtcggtacat tatttgtctg
     4261 ttatatccgc gtcttctgcg tccagcacca cttcttcagg gcgcagcaca ctaatcagtt
     4321 tgaagatcaa acttaagccg ataccgacga tggtcgccag cgccatacct ttcagctccg
     4381 ccgcgccaat gtttaccttc gcgccactga caccgatgat caaaatcacg gaagtcagga
     4441 tcaggttctg tgctttgttg taatccactt ttgattcgat caaaacacga ataccggaag
     4501 caccgatgac accataaagc agcagcgaaa cgccgcccat caccggcaat gggatcatct
     4561 ggatagcggc agccagttta ccgacgcagg aaagcaggat agcgaaaatc gccgccccac
     4621 cgataaccca ggtactgtaa acacgggtga tcgccatcac gccaatgttt tctccgtaag
     4681 tggtatttgg cgtagagcca aagaagccgg agatcacagt cgacaggccg ttagcaaaca
     4741 tcgaacggtg cagacctgga tcgcgcagca gatctttttt gacgatatta gccgttacta
     4801 ccaggtgccc tacgtgttcg gcaataacca ctaacgccgc tggcagaata gtcagaatgg
     4861 caaaccactc gaagcgcggc gtatagaggg ttggcagcgc aaaccagtga gcattaataa
     4921 tcggcgtggt atcgacaatt cccattgcga aagagagcgc gtaccccacc agcacgccaa
     4981 ttaaaatcgg gataattgcc aggaaaccac ggaacagcac ggaacctaaa accgtgaccg
     5041 ccagggtggt aatagagatg atgatggttt tggagtctgg cgtttgccct tcagccggga
     5101 gtaaacccgc cataccggca gctacgcccg ccagctccag accgatgacg gcaacgattg
     5161 cgcccattgc cgcaggtgga aacagcacgt ccagccagcc ggtccccgct ttcttcacga
     5221 taaaagaaac caggcagaac agcacgccgc acataataaa gccgcccagc gcgacttcat
     5281 accctaacgg caacagtaac aataccggtg aaataaaggc aaagctggaa ccaagataag
     5341 ccggaatttt ccctttacag atgaagagat acagcagcgt tccaataccg ttaaataaca
     5401 gtacagtcgc cgggttaata tgaaataaga cgggcaccag gacggttgca ccaaacatgg
     5461 cgaacaaatg ttgcaaacta agcgggattg tctgtaaaag tggcggtctt tcactcaccc
     5521 cgatagcacg gcgcgtcata gtattatcct ctgtattatg tgttataggc gctttactca
     5581 aaaaaaagcc gactcttaaa gtcggcttta attattttta ttctttattt cgtaccaaag
     5641 attttgtcac cggcatcgcc gaggcccgga ataatgtatc cgtgctcgtt cagtccctgg
     5701 tcaatcgatg cggtatacag ttcgacgtcc gggtgcgctt tttccagcgc agcgatacct
     5761 tctggcgcag ctaccagcac cagaactttg atgctgctgc agcccgcttt tttcagcagg
     5821 tcgatggtcg cgataacgga accaccggtt gccagcattg ggtcaacgat cagcgccata
     5881 cgctcatcga tgttagaaac cagtttctgg aagtacggta ccggctccag cgtttcttca
     5941 ttacggtaca taccgacaac gctgatgcgc gcgctcggaa cattttccag cacaccgtcc
     6001 atcatcccca gaccggcacg cagaattggc acaacggtaa ttttcttacc tttgatctgg
     6061 tcgatttcta ccgggccgtt ccagccttcg atagttactt tttccgtttc gaggtcggcg
     6121 gtcgcttcgt aagtcagcag gctacccact tcggaagcga gttcgcgaaa gcgcttggtg
     6181 ctgatatctt gctcacgcat cagtcccagc ttgtgtttga cgagtgggtg tttgacttcc
     6241 acgatcttca tactctttct cctttgaggg gcagccacaa aaaaaatcga cggattatac
     6301 ctcctttctt caaggcggca atattctttt cgttgacttt agtcaaaatg ataacggttt
     6361 gagataaagt tattttatat tcaaatggtt atgaaagaag attattccat ccgaaaacta
     6421 acctttgccc tggcacaagt cttctttcgc cgcgcgtctg gggaaaagac gtgcaaaaag
     6481 gttgtgcaaa gcagtctcga aaacgtttgc tttccctgtt agaattgcgc cgaattttat
     6541 ttttctaccg caagtaacgc gtggggaccc aagcagtgac cgataaaacc tctcttagct
     6601 acaaagatgc cggtgttgat attgacgcgg gtaatgctct ggttggaaga atcaaaggcg
     6661 tagtgaagaa aacgcgtcgt ccggaagtga tgggcggtct gggcggcttc ggtgcgctgt
     6721 gtgcattgcc gcaaaaatat cgtgaacccg tgctggtttc cggcactgac ggcgtaggta
     6781 ccaagctgcg tctggcaatg gacttaaaac gtcacgacac cattggtatt gatctggtcg
     6841 ccatgtgcgt taatgacctg gtggtgcaag gtgcagaacc gctgtttttc ctcgactatt
     6901 acgcaaccgg aaaactggat gttgataccg cttcagcggt gatcagcggt atcgcggaag
     6961 gatgtctgca atcgggctgt tcactggtgg gtggcgaaac ggcagaaatg ccggggatgt
     7021 atcacggtga ggattacgat gtcgcgggtt tctgcgttgg cgtggtagaa aaatcagaaa
     7081 tcatcgacgg ctctaaagtc agcgacggcg atgtgctgat tgcactcggt tccagcggtc
     7141 cgcactcgaa cggctattcg ctggtgcgca aaattcttga agtcagcggt tgtgatccgc
     7201 aaaccaccga acttgatggt aagccattag ccgatcatct gctggcaccg acccgcattt
     7261 acgtgaagtc agtgctggag ttgattgaaa aggtcgatgt gcatgccatt gcgcacctga
     7321 ccggcggcgg cttctgggaa aacattccgc gcgtattgcc agataatact caggcagtga
     7381 ttgatgaatc ttcctggcag tggccggaag tgttcaactg gctgcaaacg gctggtaacg
     7441 ttgagcgcca tgaaatgtat cgcaccttca actgcggcgt cgggatgatt atcgccctgc
     7501 ctgctccgga agtggacaaa gccctcgccc tgctaaatgc caacggtgaa aacgcgtgga
     7561 aaatcggtat catcaaagcc tctgattccg aacaacgcgt ggttatcgaa taatgaatat
     7621 tgtggtgctt atttccggca acggaagtaa tttacaggca attattgacg cctgtaaaac
     7681 caacaaaatt aaaggcacca tacgggcagt tttcagcaat aaggccgacg cgttcggcct
     7741 tgaacgcgcc cgccaggcgg gtattgcaac gcatacgctc atcgccagcg cgtttgacag
     7801 tcgtgaagcc tatgaccggg agttgattca tgaaatcgac atgtacgcac ccgatgtggt
     7861 cgtgctggct ggttttatgc gcattctcag cccggcgttt gtctcccact atgccgggcg
     7921 tttgctgaac attcaccctt ctctgctgcc gaaatatccc ggattacaca cccatcgtca
     7981 ggcgctggaa aatggtgatg aagagcacgg tacatcggtg catttcgtca ccgatgaact
     8041 ggacggtggc ccggttattt tacaggcgaa agtcccggta tttgctggtg atacggaaga
     8101 tgacatcacc gcccgcgtgc aaacccagga acacgctatt tatccactgg tgattagctg
     8161 gtttgccgat ggtcgtctga aaatgcacga aaacgccgcg tggctggatg gtcaacgtct
     8221 gccgccgcag ggctacgctg ccgacgagta atgccccgta gttaaagcgc cagctctgcc
     8281 gctggcgttt ttcaattcac ctgtaaatcg caagctccag cagttttttt cccccttttc
     8341 tggcatagtt ggacatctgc caatattgct cgccataata tccaggcagt gtcccgtgaa
     8401 taaaacggag taaaagtggt aatgggtcag gaaaagctat acatcgaaaa agagctcagt
     8461 tggttatcgt tcaatgaacg cgtgcttcag gaagcggcgg acaaatctaa cccgctgatt
     8521 gaaaggatgc gtttcctggg gatctattcc aataaccttg atgagttcta taaagtccgc
     8581 ttcgctgaac tgaagcgacg catcattatt agcgaagaac aaggctccaa ctctcattcc
     8641 cgccatttac tgggcaaaat tcagtcccgg gtgctgaaag ccgatcagga attcgacggc
     8701 ctctacaacg agctgctgct ggagatggcg cgtaaccaga tcttcctgat taatgaacgc
     8761 cagctctccg tcaatcaaca aaactggctg cgtcattatt ttaagcagta tctgcgtcag
     8821 cacattacgc cgattttaat caatcctgac actgacttag tgcagttcct gaaagatgat
     8881 tacacctatc tggcggtgga aattatccgt ggcgatacca tccgttacgc gctgctggag
     8941 atcccatcag ataaagtgcc gcgctttgtg aatttaccgc cagaagcacc gcgtcgacgc
     9001 aagccgatga ttcttctgga taacattctg cgttactgcc ttgatgatat tttcaaaggc
     9061 ttctttgatt atgacgcgct gaatgcctat tcaatgaaga tgacccgcga tgccgaatac
     9121 gatttagtgc atgagatgga agccagcctg atggagttga tgtcttccag tctcaagcag
     9181 cgtttaactg ctgagccggt gcgttttgtt tatcagcgcg atatgcccaa tgcgctggtt
     9241 gaagtgttac gcgaaaaact gactatttcc cgctacgact ccatcgtccc cggcggtcgt
     9301 tatcataatt ttaaagactt tattaatttc cccaatgtcg gcaaagccaa tctggtgaac
     9361 aaaccactgc cgcgtttacg ccatatttgg tttgataaag cccagttccg caatggtttt
     9421 gatgccattc gcgaacgcga tgtgttgctc tattatcctt atcacacctt tgagcatgtg
     9481 ctggaactgc tgcgtcaggc ttcgttcgac ccgagcgtac tggcgattaa aattaacatt
     9541 taccgtgtgg caaaagattc acgtatcatc gactcgatga tccacgctgc gcacaacggc
     9601 aagaaagtga ccgtggtggt tgagttacag gcgcgtttcg acgaagaagc caacattcac
     9661 tgggcgaagc gcctgaccga agcaggcgtg cacgttatct tctctgcgcc ggggctgaaa
     9721 attcacgcca aactgttcct gatttcacgt aaagaaaacg gtgaagtggt ccgttacgca
     9781 cacatcggga ccgggaactt taacgaaaaa accgcgcgtc tttatactga ctattcgttg
     9841 ctgaccgcag atgcgcgtat caccaacgaa gtacggcggg tatttaactt tattgaaaac
     9901 ccataccgcc cggtgacatt tgattattta atggtgtcac cgcaaaactc tcgccgtctg
     9961 ttatatgaaa tggtagaccg cgaaatcgcc aacgcgcagc aagggctgcc cagtggtatc
    10021 accctgaagc taaataacct tgtcgataaa ggcctggttg atcgtctgta tgcggcctcc
    10081 agctccggcg taccggttaa tctgctggtt cgcggaatgt gttcgctgat ccccaatctg
    10141 gaaggcatta gcgacaacat tcgtgccatc agtattgttg accgttacct tgaacatgac
    10201 cgggtttata tttttgaaaa tggcggcgat aaaaaggtct acctttcttc cgccgactgg
    10261 atgacgcgca atattgatta tcgtattgaa gtggcgacac cgctgctcga tccgcgcctg
    10321 aagcagcggg tgctggacat catcgacata ttgttcagcg atacggtcaa agctcgttat
    10381 atcgataaag aactcagtaa tcgctacgtt ccccgcggca atcgccgcaa agtacgggcg
    10441 cagttggcga tttatgacta catcaaatca ctcgaacaac ctgaataacc ctatgccaat
    10501 acacgataaa tcccctcgtc cgcaggagtt tgctgcggtc gatcttggtt caaacagttt
    10561 tcacatggtc atagcccgtg tggtagatgg tgccatgcag attattggcc gcctgaaaca
    10621 gcgggtgcat ctggcggacg gcctggggcc agataatatg ttgagtgaag aggcaatgac
    10681 gcgcggtttg aactgtctgt cgctgtttgc cgaacggcta caagggtttt ctcctgccag
    10741 cgtctgtata gtcggcaccc atacgctgcg tcaggcgctg aacgccactg actttctgaa
    10801 acgcgcggaa aaggtcattc cctacccgat tgaaattatt tccggtaatg aagaagcccg
    10861 tctgattttt atgggcgtgg aacataccca accggagaaa ggtcgcaaac tggttattga
    10921 tattggcggc ggatctacgg aactggtgat tggtgaaaat ttcgaaccga ttctcgttga
    10981 aagccgccgg atgggttgtg tcagctttgc ccagctttac ttccctggcg gggtcatcaa
    11041 taaagagaat tttcagcgcg ctcgtatggc ggcagcacaa aaactggaaa ctttaacctg
    11101 gcaattccgt attcagggct ggaacgtggc aatgggcgct tccggtacca taaaagccgc
    11161 ccatgaagtg ttaatggaaa tgggcgagaa agacgggata attaccccgg aacgtctgga
    11221 aaaactggta aaagaagttt tacgtcaccg taatttcgca tcgctgagtt taccgggtct
    11281 ttccgaagag cggaaaacag tcttcgttcc tggactggcg attttatgcg gtgtgtttga
    11341 tgctttagcc atccgtgaac tgcgcctttc tgacggggcg ttacgcgaag gcgtactgta
    11401 cgaaatggaa ggacgtttcc gtcatcagga tgtgcgtagt cgcaccgcca gcagcctcgc
    11461 caaccagtat cacatcgaca gcgaacaggc ccgacgggtg ctggatacca ctatgcaaat
    11521 gtacgaacag tggcgggaac agcaaccgaa gctggcgcat ccgcaactgg aggcgctact
    11581 gcgatgggcc gccatgctgc atgaggtcgg gttgaatatc aaccacagcg gtttgcatcg
    11641 ccactccgct tatattctgc aaaacagtga cttgccgggt tttaatcagg aacagcagct
    11701 gatgatggcg acactggtgc gctatcaccg taaagcgatt aaactcgacg atctgccgcg
    11761 ctttaccttg tttaagaaga aacagttcct gccactgatt cagctattgc gccttggcgt
    11821 attactcaac aatcaacgtc aggcaaccac cacaccgcca acattgacac tgattactga
    11881 tgacagtcac tggacactgc gtttcccgca tgactggttt agtcagaatg cgttggtact
    11941 gcttgatctg gaaaaggagc aagaatactg ggaaggcgtg gctggctggc ggttgaaaat
    12001 tgaagaagaa agtacacctg aaatcgccgc ttaatgcttt gcgggccgac gagaaatgtc
    12061 ggcccgcatt attcaggcac tttcgcgaat gggttcgatt tcattcaacg tatcaattaa
    12121 cggctgcggc ttaccaataa gataaccctg catataatcg atccccaaag agagcaccgc
    12181 ctcgcggatc tcttcgtttt caacgtactc tgccactacc cgcattttct tcattcgcgc
    12241 cagatggcaa atcgacgcca caatctgata atccagacta tttgacacaa tattgcggat
    12301 aaagctgccg tcaattttaa gcagatcggc attcacattt ttaagccgtg catagctggc
    12361 gtagccggtg ccgaaatcat caatcgcaat ctggcagcct aattcctgaa gatgctgcaa
    12421 ggtgatttgc gcctgcttaa cattggtcag agcattactt tcggtgactt caaaaataag
    12481 ttgccacgct tcaatctgat atttagccag caactgactg acttcaacag gaaaacgagc
    12541 ctgacatacc gaggttggag acagattaat agcaaaacgg tgagcgggca tcttcgctct
    12601 gttttcagcc ataaattgca gcgtatgctc aatgacccac atgtcgatac tcgacgataa
    12661 accaaattcg tgcgcgaccg gcaagaagct atcggggacg atcagttcat cattctcacc
    12721 tttcatgcgc agaagaattt catggtaaac atcaccacgc ataccggtaa tcggctgggc
    12781 catcaggaaa aaatggttgt gttccagcgc ctgctgtagt cgattcatca tcgcgacttt
    12841 atctttcaat tcgcgttgca aatacattgc cccgcgacgc tgcatatttt ccggggcgtt
    12901 ggtcacgatg gaaagttcgg cgaccgtatt tagctctccc agcagcaggt agatatgatt
    12961 cactggcgag cgcacatagc agtaactgac gccaatctgc ggttgcatcg gcatcccatc
    13021 ccaaaagaaa cgaaattgct tgagatgact atccagtgcg gtaatgcgct cctggtgcga
    13081 ttctgtattc agtcgcagcg cgagatcgtt acccgaaagc tgataaacat cttcacccgg
    13141 ttccagcaag ggtgacagcc agtgagaaag tttttgcttg tattgaatcc gcagcatgat
    13201 gccatagttc ttaaccagca tttccatgcc agggatgcgt aaataacaaa gcgcagacca
    13261 gggagcatca cgcaacgcgc gattcagggc gcgaacattt ggcagatgaa ccaccgggtc
    13321 catatacgca agccgctgaa tgcgtctgac taccgctctc tgacgggttg ccagtacagc
    13381 catataattg acaataaaag agaataccag ataactggag gaggttatgg tcagctgcgt
    13441 ggtatagcca ggataaatgg gaatgtaatt ttgatagctg tggatgctga tcatcaacac
    13501 gaccgcccag agcagcgaaa tcagcttata accatagcgc atcgctcccc acatcatcag
    13561 gggcagcaat aatgacaagg tataattggt gctaaaaatt gtgctttttt catttaacgg
    13621 catgcataac agcaacaata aagcacctaa tgccagtagc cagagcgcga actctttttt
    13681 ggtgactttg gcatcaacct gctgttttaa ttgcgaataa tagctacgta aataaaatgg
    13741 atttcgcact acccgaatga tgaagtagca cagcgggaca ccgatcagat tacccaccag
    13801 taaggcctga taattgatta aagtcccgag gttaaaaggc atgacaccga ccagattttc
    13861 tctgctcgcc agtaatccta caaatgcagc aaactggaaa agtatcagaa acagcgttgc
    13921 aggaaacaca atctgccaga atatacgctg ggaaattaaa cgggtatcac catgtgaaac
    13981 gttgttacgc cggggagtaa agaccctgta accgccccaa cagagaatga taatgataat
    14041 aaaattagcc gttaatgata gcgtttcgta aaaacccagc tctgcatatt tgcggacaaa
    14101 aatccctaag gcgatccctg gcaatgccgc ccagctgaaa aacatcatca tgctgatcat
    14161 cagtgccaga ggcagataaa agagaaaaac ctcaccggaa gaaatatgcg cgaaagtatt
    14221 aatgtgggcg aaaatgggca agattaaaga aggcaggaac agcggaagcc cccaccattt
    14281 atcacgcatt tttatataag ttgcattcag tttcatagat gctcagcaga atcccccaca
    14341 tcctgaagga ggtgtattca gacaggcatc ccacctgact tcgaatgatg attattcatc
    14401 actatagaga gcattgattc taagtgtcat atgaaagttc caattgatat atatcaaaca
    14461 aaataaccct gattaatgaa ttattacgtt tatcatgtta attcatcatt attacatcat
    14521 cattgtaaat aattaaatta acttccataa cattaaaata tgtatccact gacgtttttt
    14581 tacataacga agaattgacc attttgtcct gttgtgcctt aatgtaagta ccgtccacag
    14641 cgtgggacat acttcaagga accttttgtg agtcaggcaa ccagtatgcg aaaacgacac
    14701 cgatttaaca gtcgcatgac ccgtatcgta ctgctcatca gctttatctt cttctttggc
    14761 cgttttatct actcgtccgt cggtgcctgg cagcaccatc agagcaaaaa agaagctcag
    14821 caatccacac tctccgtcga atcaccggta caacgttagc ggttaccttc ttcactttca
    14881 cagaacataa cggcacttcg ctgtcggatg cttttgctct ttgggattat caaagcggca
    14941 gatattcttt catcttaaat tttacgtctt tgtcctgact gatgtttatc ctgtttg
//
LOCUS       DAZPDU010000107        14986 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZPDU010000107 DAZPDU010000000
VERSION     DAZPDU010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14986
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.108"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            181..423
                     /gene="yjbE"
                     /locus_tag="ACHURS_002891"
     CDS             181..423
                     /gene="yjbE"
                     /locus_tag="ACHURS_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFR1238567.1"
                     /translation="MKKVLYGIFAISALAATSVWAAPVQVGEAAGAAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            537..1175
                     /locus_tag="ACHURS_002892"
     CDS             537..1175
                     /locus_tag="ACHURS_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFR1238568.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1172..1909
                     /locus_tag="ACHURS_002893"
     CDS             1172..1909
                     /locus_tag="ACHURS_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFR1238569.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALEVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1909..4005
                     /locus_tag="ACHURS_002894"
     CDS             1909..4005
                     /locus_tag="ACHURS_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFR1238570.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTIGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDAYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(4052..4330)
                     /locus_tag="ACHURS_002895"
     CDS             complement(4052..4330)
                     /locus_tag="ACHURS_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1238571.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4331..4474
                     /locus_tag="ACHURS_002896"
     CDS             4331..4474
                     /locus_tag="ACHURS_002896"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1238572.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPAWVRFVLPDAPPGAAYP
                     AYG"
     gene            4600..5010
                     /gene="psiE"
                     /locus_tag="ACHURS_002897"
     CDS             4600..5010
                     /gene="psiE"
                     /locus_tag="ACHURS_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFR1238573.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(5054..6529)
                     /gene="xylE"
                     /locus_tag="ACHURS_002898"
     CDS             complement(5054..6529)
                     /gene="xylE"
                     /locus_tag="ACHURS_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFR1238574.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(6901..7791)
                     /gene="malG"
                     /locus_tag="ACHURS_002899"
     CDS             complement(6901..7791)
                     /gene="malG"
                     /locus_tag="ACHURS_002899"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFR1238575.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     AIGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(7806..9350)
                     /gene="malF"
                     /locus_tag="ACHURS_002900"
     CDS             complement(7806..9350)
                     /gene="malF"
                     /locus_tag="ACHURS_002900"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFR1238576.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ECAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGEIGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLLFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(9504..10694)
                     /gene="malE"
                     /locus_tag="ACHURS_002901"
     CDS             complement(9504..10694)
                     /gene="malE"
                     /locus_tag="ACHURS_002901"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFR1238577.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVAVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            11059..12174
                     /gene="malK"
                     /locus_tag="ACHURS_002902"
     CDS             11059..12174
                     /gene="malK"
                     /locus_tag="ACHURS_002902"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFR1238578.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            12246..13586
                     /gene="lamB"
                     /locus_tag="ACHURS_002903"
     CDS             12246..13586
                     /gene="lamB"
                     /locus_tag="ACHURS_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFR1238579.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            13738..14658
                     /gene="malM"
                     /locus_tag="ACHURS_002904"
     CDS             13738..14658
                     /gene="malM"
                     /locus_tag="ACHURS_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFR1238580.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            14887..>14986
                     /locus_tag="ACHURS_002905"
     CDS             14887..>14986
                     /locus_tag="ACHURS_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1238581.1"
                     /translation="MIGHITNSGGNNSLMKHDVVQGNNIVDLDLLRN"
BASE COUNT         3730 a         3837 c         3922 g         3497 t
ORIGIN      
        1 tatgattttt attgttattt aaatataagt tgaaacttat atttgatatt cattccaatt
       61 atcctaaaac accatcgcta attccccgcg ccgtaattcg catgctttag ttgtgtatac
      121 tcgatcccgc ccgaaatgtt tttgggtaaa tctccattca ttcaatgaag ggaaattgtt
      181 atgaaaaaag ttctgtatgg catttttgcc atatctgcgc ttgcggcgac ttctgtgtgg
      241 gctgcacctg tacaggtggg cgaagcggca ggggcggcag caacgtcggt ttcggcgggg
      301 agttcctccg cgaccagcgt cagcaccgta agctcggcgg tgggtgtcgc gctcgcggca
      361 accggtggcg gtgatggttc taataccggg accacaacaa ccacaaccac cagtacccag
      421 taataaagta tgtatcccca aaataattcg agtcattgca tctgtggctt gaaatatgaa
      481 gggattaacc ataaccacac tccggtgtgg ttattctgcc cctctggaga agagtcgtga
      541 agcgacctgc actcattctt atctgcctgt tattacaggc ctgttcagcc acgactaaag
      601 agctgggcaa ttcactgtgg gacagtctgt tcggcacgcc aggcgtacag ctgacggacg
      661 atgatattca aaatatgccc tacgccagcc agtacatgca gcttaatggc gggccgcaat
      721 tgtttgtggt gctggccttc gctgaagacg gacaacaaaa atgggtcact caggatcaag
      781 ccactcttgt cacacaacat ggtcgtctgg tgaagacttt gcttggcggc gacaacctga
      841 ttgaagtgaa taaccttgcc gccgacccgc tgattaaacc cgcacaaatt gttgatggtg
      901 caacatggac gcgcacgatg ggctggaccg agtaccagca ggtacgctac gccaccgcac
      961 gctcagtctt caaatgggat ggcaccgata ccgtcaaggt cggcagcgat gaaaccctgg
     1021 ttcgcgtgct ggacgaagaa gtctccaccg accaggcgcg ctggcataac cgctactgga
     1081 tcgacagcga agggcaaatt cgccagtcgg aacagtatct cggcgcggat tatttcccgg
     1141 tgaaaaccac tctcatcaag gcggcaaaac aatgattaaa caaactattg tcgcgttgct
     1201 tttgagtgtg ggagcgtcat cggtcttcgc ggcaggaacc gtcaaggtgt tcagcaatgg
     1261 cagcagtgag gccaaaacgc taacgggcgc agagcattta atcgatctgg tagggcaacc
     1321 tcgactggca aacagctggt ggcctggtgc ggtgattagc gaagagctgg caacggcggc
     1381 agcattgcgt cagcagcagg cgttgctgac acggctggca gaacagggcg cagattccag
     1441 caccgacgat gccgccgcca ttaacgcctt acgccagcaa atccaggcat tggaagtgac
     1501 aggcaggcaa aaaatcaatc ttgatcccga tatcgtccgc gttgccgaac gcggtaaccc
     1561 gccgttgcag ggcaactaca cgctgtgggt cggaccaccg ccgtccacgg tcacgttgtt
     1621 cgggcttatc agccatcctg gcaatcagcc attcactcca ggtcgcgacg tggcgagcta
     1681 tctctctgac cagagcctgc tcagcggtgc ggatcgcagc tacgcgtggg tcgtttaccc
     1741 ggacggacgc acgcaaaaag cgccggtggc ttactggaac aagcgtcacg tagagccgat
     1801 gcccggcagc attatttatg ttggcctcgc agactccgtc tggagtgaga cgcctgatgc
     1861 ccttaacgcc gacattcttc agactctgac gcagcggata ccccaataat gaaaaaaaga
     1921 catctgctta gcttactggc gctgggcatt agcacagctt gctacggcga aacatatcct
     1981 gcgcccattg gtccgtcgca gtcggatttc ggtggcgtag gattattaca aactcccacc
     2041 gcgcgtatgg cacgggaagg ggagttgagt ctgaactatc gcgataacga tcagtaccgt
     2101 tattactcag cttcagtgca actcttcccg tggctggaaa caacgctgcg ttacaccgac
     2161 gtgcgcaccc ggcagtacag cagcgtcgaa gcgttctctg gcgatcaaac gtataaagat
     2221 aaagccttcg atctcaaact gcgtttgtgg gaagagagtt actggctgcc gcaagtggcg
     2281 gttggtgcgc gggatatcgg cggtacgggg ctgtttgatg cggaatatct tgttgccagc
     2341 aaagcctggg ggccgttcga ttttacgctc ggcctgggct ggggatatct gggcaccagc
     2401 ggtaatgtga aaaatccgct ctgttcagcc agtgataaat attgctatcg cgataacagc
     2461 tacaaacaag cgggatctat cgacggcagt cagatgttcc acggcccggc ctcactgttt
     2521 ggcggcgtgg aataccagac gccctggcaa ccgctgcgcc tgaaactgga gtatgaaggt
     2581 aataattatc agcaggattt tgccgggaag ctggagcaaa aaagtaagtt taacgtcggg
     2641 gcgatttatc gcgttaccga ttgggccgac gttaacctta gctatgaacg tggcaacacc
     2701 tttatgtttg gcgtcacgct gcgcaccaac tttaacgatc tgcgcccgtc ctacatcgat
     2761 aacgcccgcc cgcaatatca accgcagccg caggatgcca ttttgcagca ctcggtggtg
     2821 gcgaatcagt taacgttgct gaaatacaat gccggacttg ccgatccgca gatccaggcg
     2881 aaaggcgata cgctgtatgt taccggcgag caggtgaaat atcgtgattc gcgcgaaggg
     2941 atcatccgtg ctaatcggat cgtgatgaac gatctgccgg atgggatcaa aacgatccgc
     3001 attacggaaa atcgccttaa catgccgcag gtgacgacgg aaaccgatgt cgccagcctg
     3061 aaaaatcatc tcgccggaga gccgttgggc cacgaaacga cgctggcgca aaaacgcgtc
     3121 gagccagtgg ttccgcagtc caccgagcag ggctggtata tcgacaaatc acgctttgat
     3181 ttccatatcg atccggtgct gaaccagtcg gtcggtggcc cggaaaactt ttacatgtat
     3241 cagctgggcg tgatgggaac ggcagatttg tggctgacgg accatctgct gaccatcggc
     3301 agcctgtttg caaatcttgc caacaactac gacaagttta actacaccaa tccgccacag
     3361 gactcgcact taccgcgcgt tcgtacccat gtgcgcgagt atgtgcagaa cgatgcctat
     3421 gtgaataacc tgcaagccaa ctacttccag catctgggca acggcttcta cggtcaggtc
     3481 tatggaggtt atctcgaaac catgtttggc ggtgcggggg ctgaagtgtt gtatcgtccg
     3541 ctggacagca actgggcgtt tggtctggat gccaactacg ttaaacagcg cgactggcgt
     3601 agtgcaaaag atatgatgaa attcaccgac tacagcgtga aaaccggaca tctgaccgcc
     3661 tactggacgc catctttcgc ccaggacgtg ttagtgaaag ccagcgtcgg gcagtatctg
     3721 gcaggggata aaggcggcac gctggagatc gccaaacgct ttgatagcgg cgtggtggtg
     3781 ggtggctatg ccacgatcac gaatgtttca aaagaggagt acggcgaagg ggacttcacc
     3841 aaaggcgtgt atgtctcggt accgttggat ctcttctcgt ctggcccgac acgcagccgt
     3901 gcggcgattg gctggacgcc gctgacgcgt gacggtggtc agcaacttgg gcgtaagttc
     3961 cagttgtatg atatgaccag cgaccgtagc gtcaatttcc gctaagtcat gggaaaggtg
     4021 ccagttttcg caatcactgg caccctcatt cttaatggcc tgttgagcca tcaggaacgc
     4081 agttcacccc cggtttgcga tctggtgtgt aaaggcgaaa acattctgtc ttcctgtccg
     4141 tcgatatcgg ttgcaggtct ggttgcctga gcgactgggc catcattggg ctggagacat
     4201 tgctttgcgg taggtcggct ttatcagcag taccgagcgg accagcataa gcaggaagaa
     4261 cagagactga taacatcaaa gcagcaaaat aaggcttcat ttttaccacc tttatcaggt
     4321 tacgtttcat ttgttccaga ggaacattgt cgatttttcg cgcattgctg gtggctggga
     4381 atcacctgaa tgggtgattt ttgaattacc ggcttgggtg cggtttgtct tgccggatgc
     4441 gccgccaggc gcggcttatc cggcctacgg gtaggtatat ccggctttgg tggaggcgcg
     4501 ctccaaatcc aggttgaaca aaacatacac aaaaaatata gatctccgtc acatttttgc
     4561 gttatacagg aagctcgcca ctgtgaagga ggtactgcta tgacgtcact ctctcgtccg
     4621 cgcgtggagt ttatctccac cattttgcag accgtactca atcttggcct gctgtgcctc
     4681 ggcctgattt tggttgtctt cctcggcaaa gaaacggtgc atctggcaga tgtgctgttc
     4741 gcgccagaac aaaccagcaa atatgagctg gtagaaggac tggtggttta ctttctctat
     4801 ttcgaattta tcgcgctgat tgtgaagtac tttcagtccg gttttcactt cccgctacgt
     4861 tactttgtct atattgggat caccgccatt gtgcgcttga tcatcgtcga tcataaatcg
     4921 ccactggatg tgttgatcta ctcggctgcg atcctgttac tggtgatcac tctgtggctg
     4981 tgtaattcga aacggctgaa gcgggagtaa aaagaaagca cgccgaactg gcgcggcgtg
     5041 ctggacagga agattacagc gtagcagttt gttgtgtttt cttcgtttcc ggttcccaga
     5101 gggcttccag ctcctcaagg gttttacctt tggtttccgg gacaaatttc cacataaata
     5161 gtgctgccag aacgcccata cacccgtaaa tccagtagga gaaaccgttg tggaaatggg
     5221 ccaccagcca ggagtttttg tccatcatcg ggaaggtcca ggagacgaag tagttcgcca
     5281 gccactgggc cgccaccgcg attgccagcg ctttaccacg aatagcattc gggaagattt
     5341 ccgacagcag tacccagcat accggacccc aggacatggc aaaggcagca acatagaaca
     5401 gcatcgacag tagcgccaca atacccggtg cctgagtgta aaacgcggta ccgaggctaa
     5461 acataccgat tgccattccg agtgcgccga taatttgcag tggcttacga ccaaatttat
     5521 ccaccgtcat aattgccaga acggtgaagg tgaggttgat aactccgaca ataatggtct
     5581 gcaacagcgc gatatccgtg ctggccccca gcgttttgaa cacttccggc gcgtagtaca
     5641 gcaccacatt gatgccgaca aattgctgga agatggagag cataacgccg atgacaatca
     5701 cgcccacgcc aaacatcagc agacgaccac cggttttgcg gccatgatcc agggagtgtt
     5761 taatttcctg tactgcctga gttgcaagcg tgttgcccat aattttgcgc aggatacttt
     5821 ccgcctgttc ttgcttgccg cgtgacatca gccagcgagg actttctggc acggtataca
     5881 gcagcattaa gaacagcagt gcagggatac attccgaggc aaacatataa cgccagccgt
     5941 cagtattcag ccagctggca tcaccggaac gggcaataaa atagtttacg cagtaaacta
     6001 aaagttgccc gaaaataatc gcaaactggt taaatgaaac cagttttccg cgaatatgag
     6061 ctggagccag ttccgcaata tacattggtg aaagcattga ggctaaacca acgccaatac
     6121 cgccaataat acgataaata acaaattccg ggacataacc tgccagataa ataggtactg
     6181 tgttgtccgg gtttatagag gtaaaaccaa gttctggcca ggcagaacct acaccagaaa
     6241 taaaaaacag gacagcagca atcttaagtg aatcacgacg gccgaagcgg ttactgcaat
     6301 aaccaccgag ggcaccgccg atgatgcaac caatcagagc gctggctacg caaaatccta
     6361 acagggagtt ggcagcggat tcacttaagt tttgtggagc aacaaagacg gtattgagtg
     6421 actcaacagt accggaaata acggcggtgt cgtagccaaa taataaacca cctaatgtag
     6481 cgactaaggt aatcgaaaat atataactgg aattatactg ggtattcatt cagacctgcc
     6541 ttagaccatt ctgatctttt atcagtgcat tgatgttgga attgtcaggc agttaaggca
     6601 attatcattt tttacaatgc gtttacgtaa tgtcttttct gtgatcttaa ttgtgataat
     6661 tatcaaaaat tgatgaaaac aatactattg ccgtgactca gagcacgaaa gagaattatc
     6721 gtaagtggga aaacaaataa cgtaaaaata ataagctcta tgatggaaat attaaccggc
     6781 gaacgattca gattgcagac gaaagaaaaa aaggcgctcc gtggagcgcc gaataacagt
     6841 cacaagttgg gataacgtaa gttgagggtg cagcggcata acattggcag aacaacatct
     6901 ttaacctttc acaccacctg ccgtcaggcc gttgaccagc cagcgttgag ccagcaagaa
     6961 gacgatggtg atcggtaatg cagacatcac ggcagcggcg gcaaagtcac cccacaggta
     7021 gttttgcggg ttgaggtatt gctgcatccc cacggccagg gtgtagctgt ttacgtcacg
     7081 cagtaacagc gacgcgaccg gaacttcagt aatggcagcg ataaacgaca ggatgaatac
     7141 caccgccaga atcggtactg acagcggcaa caggacaagg cggaaggcct gccacggtgt
     7201 cgcaccatcc agcgcagccg cttcttccag cgaactatcg atggtttcga aatagccttt
     7261 gatggtccag acatgcagcg caatcccacc cagatacgcg aaaattacgc cgccgtgagt
     7321 attcaggcca atgaatggaa tgtactcacc cagacgatca aacaacgcat acaacgcgac
     7381 cagtgaaagt actgccggga acatctggaa aatcagcatt cctttcagca gcgtcgcttt
     7441 gcctggaaag cgcatacggg cgaaagcgta ggcgcaggtg gtagagagcg cgacaatgcc
     7501 aatcgcggaa atcccggcga cctttaccga gttccatagc cacagcagta ccgggaatgg
     7561 cggtggcgta atgcgaccat cagcctgttc cacgctaaaa cctaaagcca gtttccagtg
     7621 atcccaggag atttgctccg ggatcaggct gccgatcgca aagttcccct ggcgcagtga
     7681 gatagcgacg accatcagca gcgggaacat aatcgccgcg ataaaaagta gcagtagcag
     7741 gtgagtaata aacaaacgtg ctttttgcga tttcggttgg accattgcca tttttgttat
     7801 ctcccttaat caaacttcat tcgcgtggct ttcaggttca ctatcgccag cgcacccacc
     7861 agcaggaaga gcagcgtggc aattgctgca gccagaccga agtcctgacc cccgccgcct
     7921 tcaaaagcga tacggtaggt gtagttaaca agcaggtcgg tataaccggc tggcgtggtc
     7981 gtgccaagac gatccgggcc gccgttggtt aacagttgaa tcagcacgaa gttgttaaag
     8041 ttaaaggcga agctggcgat catcagcggc gtcagcggtt taatcagcag cggcagcgta
     8101 atcttaaaga agttctggaa cggacctgcg ccatccattg ctgaggcttc atacaaatcg
     8161 tccggaatcg ctttcagcaa gcccatgcag aggatcatca tgtacggata acccagccag
     8221 gtattgacga taattagcat cgtgcgggcg gtggtcggat cgctgaacca ggcgggcttc
     8281 acgccaaaca gcgcgctcaa catcatgttg atttcaccga agctctggtt aaacaaccct
     8341 ttgaaaatca agattgaaat gaatgatggc accgcgtagg gcagaatcag cagaacgcga
     8401 tagaccgctt tgccgcgcaa tgcttcccac tgcaccagac atgccagaac catgccgacc
     8461 gccaccgtta aaaagacagt gatcagcgag aacaccacgg tccagacgaa aatggcgagg
     8521 aacggtttct gaatgccttc gtcggtgaag acgcgggtaa agtttttcca gccagtagtc
     8581 acggtgtaac cggggcttag cttttcatca ccccagttgc cgtcggcggt aatggactgg
     8641 taaaagccaa tttggttatt cggacgatat ttcacgccgc tctgattatt cgtcaacgtg
     8701 ccgtcaccgt cgagtgtgta gagcggctgc gtgccagaaa actggcgcag ggagctcatc
     8761 atcactttgt tgccatccgg cagaatggcg gtaatgtcac tcagcgcctg acgattctgg
     8821 gtaatcacgc gtagattcgc gcgttcgcct tcgggctggg cggtcgtttc tttcagttgc
     8881 agtttttgtt cgccgccaaa tttaaacgcg tcggagaggt aatttttgcc gatttcgccg
     8941 tcactgagcg ccagttgcca ctcatcgccc gccgggtaaa gaccaaagtt ataggttttg
     9001 cctgcttgcc aggagcgatc taacaacact tcctgcgcac attcaaaagt cagctggtta
     9061 gtgctgctgt agttggtgaa ggcaatggcg atggtgcaga ccagagggaa gaggacgaat
     9121 aatcccattc cagccattcc cgggtaaaca tagcgccagg cgtaggcttt acgattggcg
     9181 aaaatataca gccccgctga actcaatatc agcgtggtaa tggcgaacag gtattcccct
     9241 tgtgcgtaca ttaaaacaac aaggtaaccc accagcaggc cgagcagacc tagcactgac
     9301 catttcagcg cgtcgctttg ccaccaatgt ttctttttaa tgacatccat ggggttcttc
     9361 ctcattccag gacggataag gctttcacgc cttatccgac aacaactgcc tgatgcgaca
     9421 ctgatgcgtc ttatcaggcc tacatgcgtt tcagttttgt aggccggaca aggcgttcac
     9481 gccgcatccg gcatttcaca gcattacttg gtgatacgag tctgcgcgtc tttcagggct
     9541 tcatcgacag tctgacgacc gctggcggcg ttgatcaccg cagtacgcac ggcataccag
     9601 aaagcggaca tctgcgggat gttcggcatg atttcacctt tctgggcgtt ttccatggtg
     9661 gcggcaatac gtggatcttt cgccaactct tcctcgtaag acttcagcgc cactgcaccc
     9721 agcggtttgt ctttattgac cgcttccaga ccttcgtcag tcagcagata gttttcgagg
     9781 aactctttcg ccagctcttt gttcggactg gcggcgttaa taccggcgct cagcacgcca
     9841 acgaacggtt tagatggttg acccttgaag gtcggcagta ccgttacacc ataattcact
     9901 ttgctggtgt cgatgttgga ccatgcccac gggccgttga tggtcatcgc tgtttcgcct
     9961 ttattaaagg cagcttctgc gatggagtaa tcggtgtctg cattcatgtg tttgttttta
    10021 ataaggtcaa ccaggaaggt cagacccgct ttcgcgccag cgttatccac ggccacgtct
    10081 ttaatatcgt acttgccgtt ttcatacttg aacgcataac ccccgtcagc agcaatcagc
    10141 ggccaggtga agtacggttc ttgcaggttg aacatcagcg cgctcttacc tttcgctttc
    10201 agttctttat ccagcgccgg gatctcttcc caggtttttg gtgggttcgg cagcagatct
    10261 ttgttataaa tcagcgataa cgcttcaaca gcgatcgggt aagcaatcag cttgccgttg
    10321 taacgtacgg catcccaggt aaacggatac agcttgtcct ggaacgcttt gtccggggtg
    10381 atttcagcca acaggccaga ttgagcgtag ccaccaaaac ggtcgtgcgc ccagaagata
    10441 atgtcagggc catcgccagt tgccgcaacc tgagggaatt tctcttccag tttatccgga
    10501 tgctcaacgg tgactttaat tccggtatct ttctcgaatt tcttaccgac ttcagcgaga
    10561 ccgttatagc ctttatcgcc gttaatccag attaccagtt taccttcttc gattttggcg
    10621 agagccgagg cggaaaacat catcgtcgtt aatgcggata atgcgaggat gcgtgcacct
    10681 gtttttattt tcataatcta tggtccttgt tggtgaagtg ctcgtgaaaa cacctaaacg
    10741 gactctagtt tctttatacg gcaacctctt tccatcctcc ttgcccctac gccccaccgt
    10801 tgctttgtgt gatctctgtt acagaattgg cggtaatgtg gagatgcgca cataaaatcg
    10861 ccacgatttt tgcaagcaac atcacgaaat tccttacatg acctcggttt agttcacaga
    10921 agccgtgttc tcatcctccc gcctcctccc ccataaaaaa gccagggggt ggaggattta
    10981 agccatctcc tgatgacgca tagtcagccc atcatgaatg ttgctgtcga tgacaggttg
    11041 ttacaaaggg agaagggcat ggcgagcgta cagctgcaaa atgtaacgaa agcctggggc
    11101 gaggtcgtgg tatcgaaaga tatcaatctc gatatccatg aaggtgaatt cgtggtgttt
    11161 gtcggaccgt ctggctgcgg taaatcgact ttactgcgca tgattgccgg gcttgagacg
    11221 atcaccagcg gcgacctgtt catcggtgag aaacggatga atgacactcc gccagcagaa
    11281 cgcggcgttg gtatggtgtt tcagtcttac gcgctctatc cccacctgtc agtagcagaa
    11341 aacatgtcat ttggcctgaa actggctggc gcaaaaaaag aggtgattaa ccaacgcgtc
    11401 aaccaggtgg cggaagtgct gcaactggcg catttgctgg atcgcaaacc gaaagcgctc
    11461 tccggtggtc agcgtcagcg tgtggcgatt ggccgtacgc tggtggccga gccaagcgta
    11521 tttttgctcg atgaaccgct ctccaacctc gatgctgcac tgcgtgtgca aatgcgtatc
    11581 gaaatctccc gtctgcataa acgcctgggc cgcacaatga tttacgtcac ccacgatcag
    11641 gtcgaagcga tgacgctggc cgacaaaatc gtggtgctgg acgccggtcg cgtggcgcag
    11701 gttgggaaac cgctggagct gtaccactat ccggcagacc gttttgtcgc cggatttatc
    11761 ggttcgccaa agatgaactt cctgccggta aaagtgaccg ccaccgcaat cgatcaagtg
    11821 caggtggagc tgccgatgcc aaatcgtcag caagtctggc tgccagttga aagccgtgat
    11881 gtccaggttg gagccaatat gtcgctgggt attcgcccgg aacatctact gccgagtgat
    11941 atcgctgacg tcatccttga gggtgaagtt caggtcgtcg agcaactcgg caacgaaacc
    12001 caaatccata tccagatccc ttccattcgt caaaacctgg tgtaccgcca gaacgacgtg
    12061 gtgttggtag aagaaggtgc cacattcgct atcggcctgc cgccagagcg ttgccatctg
    12121 ttccgtgagg atggcactgc atgtcgtcga ctgcataagg agccgggcgt ttaagcaccc
    12181 cacaaaacac acaaagcctg tcacaggtga tgtgaaaaaa gaaaagcaat gactcaggag
    12241 atagaatgat gattactctg cgcaaacttc ctctggcggt tgccgtcgca gcgggcgtaa
    12301 tgtctgctca ggcaatggct gttgatttcc acggctatgc acgttccggt attggctgga
    12361 caggtagcgg cggtgaacaa cagtgtttcc agactaccgg tgctcaaagt aaataccgtc
    12421 ttggcaacga atgtgaaact tatgctgaat taaaattggg tcaggaagtg tggaaagagg
    12481 gcgataagag cttctatttc gacactaacg tggcctattc cgtcgcgcaa cagaatgact
    12541 gggaagctac cgatccggcc ttccgtgaag caaacgtgca gggtaaaaac ctgatcgaat
    12601 ggctgccagg ttccaccatc tgggcaggta agcgcttcta ccaacgtcat gacgttcata
    12661 tgatcgactt ctactactgg gatatttctg gtcctggtgc cggtctggaa aacatcgatg
    12721 ttggcttcgg taaactctct ctggcagcaa cccgctcctc tgaagctggt ggttcttcct
    12781 cttttgccag caacaatatt tatgactata ccaacgaaac cgcgaacgac gttttcgatg
    12841 tgcgtttagc gcagatggaa atcaacccgg gcggcacatt agaactgggt gtcgactacg
    12901 gtcgtgccaa cctgcgtgat aactatcgtc tggttgatgg cgcatcgaaa gacggctggt
    12961 tattcactgc tgaacatact cagagtgtcc tgaagggctt taacaagttt gttgttcagt
    13021 acgctactga ctcgatgacc tcgcagggta aaggtctgtc gcagggttct ggcgtcgcgt
    13081 ttgataacga aaaatttgcc tacaatatca acaacaacgg tcacatgctg cgtatcctcg
    13141 accacggtgc gatctccatg ggcgacaact gggacatgat gtacgtgggt atgtaccagg
    13201 atatcaactg ggataacgac aacggcacca agtggtggac cgtcggtatt cgcccgatgt
    13261 acaagtggac gccaatcatg agcaccgtga tggaaatcgg ctacgacaac gtcgaatccc
    13321 agcgcaccgg cgacaagaac aatcagtaca aaattaccct cgcacaacaa tggcaggctg
    13381 gcgacagcat ctggtcacgc ccggctattc gtgtcttcgc aacctacgcc aagtgggatg
    13441 agaaatgggg ttacgactac aacggcgata gcaaggttaa cccgaactac ggcaaagccg
    13501 ttcctgctga tttcaacggc ggcagcttcg gtcgtggcga cagcgacgag tggaccttcg
    13561 gtgcccagat ggaaatctgg tggtaatagc aaaacctggg ccggataagg cgtttacgcc
    13621 gcatccggca taaaaacagg ttgtcattat ctgaaagggg cgaaagcccc tctgattatc
    13681 gggtttagcg cgctattgcc tggctaccgc tgagctccag attttgaggt gaaaacaatg
    13741 aaaatgaata aaagtctcat cgccctctgt ttatcagcag ggttactggc aagcgcgcct
    13801 ggaattagcc ttgccgatgt taactacgta ccgcaaaaca ccagcgacgc gccagccatt
    13861 ccatctgccg cgctgcaaca actcacctgg acaccggtcg atcaatctaa aacccagacc
    13921 acccaactgg cgaccggcgg ccaacaactg aacgttcccg gcatcagtgg tccggttgct
    13981 gcgtacagcg tcccggcaaa cattggcgaa ctgaccctga cgctgaccag cgaagtgaac
    14041 aaacaaacca gcgtttttgc gccgaacgtg ctgattcttg atcagaacat gaccccatca
    14101 gccttcttcc ccagcagtta tttcacctac caggaaccag gcgtgatgag tgcagatcgg
    14161 ctggaaggcg ttatgcgcct gacaccggcg ttggggcagc aaaaacttta tgttctggtc
    14221 tttaccacgg aaaaagatct ccagcagacg acccaactgc tcgacccggc taaagcctat
    14281 gccaaaggcg tcggtaactc gatcccggat attcccgatc cggttgctcg tcataccagc
    14341 gatggcttac tgaaactgaa agtcaaaacg aactccagct ccagcgtgtt ggtaggacca
    14401 ctatttggtt cttccgctcc agctccggtt acggtaggca acacggcggc accagctgtg
    14461 gctgcacctg ctccggtgcc ggtgaagaaa agcgagccga tgctcaacga cacggaaagt
    14521 tattttaata ccgcgatcaa aaacgctgtc gcgaaaggtg atgttgataa ggcgttaaaa
    14581 ctgcttgatg aagctgaacg cctgggatcg acatctgccc gttccacctt tatcagcagt
    14641 gtaaaaggca aggggtaatt acgccccaca gtgctgattt tgcaacaact ggtgcgtctc
    14701 ctggcgcacc tttttttatg cttccttcct gggatatgag cgatttttta tagtacttca
    14761 cttcttcttc actaagaatg tccattatct caatgcctta tcagagattc ttttcctttc
    14821 gccggtagtg tctggacgct caggctactt ttccaggtta ttttatttct gtattacaga
    14881 ggttttatga taggtcatat cacaaattct ggcggcaata actctttgat gaaacatgat
    14941 gtggttcaag gaaataatat agtagatctt gatttactac gtaatt
//
LOCUS       DAZPDU010000108        14886 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZPDU010000108 DAZPDU010000000
VERSION     DAZPDU010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14886
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.109"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            261..1559
                     /gene="kgtP"
                     /locus_tag="ACHURS_002906"
     CDS             261..1559
                     /gene="kgtP"
                     /locus_tag="ACHURS_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFR1238582.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HVNVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1556..1879)
                     /locus_tag="ACHURS_002907"
     CDS             complement(1556..1879)
                     /locus_tag="ACHURS_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFR1238583.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1925..3280)
                     /gene="pssA"
                     /locus_tag="ACHURS_002908"
     CDS             complement(1925..3280)
                     /gene="pssA"
                     /locus_tag="ACHURS_002908"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFR1238584.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENSGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLKLAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3394..6054)
                     /gene="pat"
                     /locus_tag="ACHURS_002909"
     CDS             complement(3394..6054)
                     /gene="pat"
                     /locus_tag="ACHURS_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFR1238585.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGSFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPTLPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6086..6784)
                     /gene="tapT"
                     /locus_tag="ACHURS_002910"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6086..6784)
                     /gene="tapT"
                     /locus_tag="ACHURS_002910"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFR1238586.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVHRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6853..7272)
                     /gene="trxC"
                     /locus_tag="ACHURS_002911"
     CDS             complement(6853..7272)
                     /gene="trxC"
                     /locus_tag="ACHURS_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFR1238587.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7479..8516
                     /locus_tag="ACHURS_002912"
     CDS             7479..8516
                     /locus_tag="ACHURS_002912"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFR1238588.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8564..9253)
                     /gene="ung"
                     /locus_tag="ACHURS_002913"
     CDS             complement(8564..9253)
                     /gene="ung"
                     /locus_tag="ACHURS_002913"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFR1238589.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9558..9941
                     /gene="grcA"
                     /locus_tag="ACHURS_002914"
     CDS             9558..9941
                     /gene="grcA"
                     /locus_tag="ACHURS_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFR1238590.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(9997..10584)
                     /gene="eamB"
                     /locus_tag="ACHURS_002915"
     CDS             complement(9997..10584)
                     /gene="eamB"
                     /locus_tag="ACHURS_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFR1238591.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10687..11568
                     /locus_tag="ACHURS_002916"
     CDS             10687..11568
                     /locus_tag="ACHURS_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1238592.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIVDVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFAVAKELECGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
     gene            complement(11777..13111)
                     /gene="srmB"
                     /locus_tag="ACHURS_002917"
     CDS             complement(11777..13111)
                     /gene="srmB"
                     /locus_tag="ACHURS_002917"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFR1238593.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13243..13980
                     /gene="trmN"
                     /locus_tag="ACHURS_002918"
     CDS             13243..13980
                     /gene="trmN"
                     /locus_tag="ACHURS_002918"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFR1238594.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWLTQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQVGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13965..>14886)
                     /locus_tag="ACHURS_002919"
     CDS             complement(13965..>14886)
                     /locus_tag="ACHURS_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HFR1238595.1"
                     /translation="VANLEFNQFPPTALYHPQARNFLLTEALRGEGAYLKRPDGTRFM
                     PDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLGLG
                     IDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSLLE
                     CLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDYVG
                     IVRTTKRLERALRRINMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRKES
                     RGLHFTLDYPELLTHSGPSILPPGNHYINR"
BASE COUNT         3589 a         3767 c         3899 g         3631 t
ORIGIN      
        1 gatgcgacac tgcgcgtctt atactcccac atatgccaga ttcagcaacg gatacggttt
       61 ccccaacttg cccacttcca tacgtgtcct ccttaccaga aatttatcct taagctcctc
      121 aataaccatt ttcctgctaa ctaaattcat ggttaaggtt gcacaatgat atgcaacaaa
      181 tgtataacat ttcctttacc aaaaaaaata aacaaaagcg accgacaaaa gcatcggatt
      241 acggcaggag acataatggc atggctgaaa gtactgtaac ggcagacagc aaactgacaa
      301 gtagtgatac tcgtcgccgc atttgggcaa ttgtgggggc ctcttcaggt aatctggtcg
      361 agtggttcga tttctatgtc tactcgttct gttcactcta cttcgcccac atcttcttcc
      421 cttccgggaa cacgacgact caactactac aaacagcagg tgtttttgct gcgggattcc
      481 tgatgcgccc aataggcggt tggctatttg gccgcatagc cgataaacat ggtcgcaaaa
      541 aatcgatgct gctatcggtg tgtatgatgt gtttcggctc actggtaatc gcctgtttgc
      601 ctggttatga aacaatcggt acgtgggctc cggcattatt gcttctcgcg cgtttatttc
      661 agggattatc cgttggcgga gaatatggca ccagcgccac ctatatgagt gaagttgccg
      721 ttgaagggcg caaaggtttt tacgcatcat ttcagtatgt gacgttgatc ggcggacaac
      781 tgctagccct actggttgtc gtggttttac aacacaccat ggaagacgct gcactcagag
      841 agtggggatg gcgtattcct ttcgcgttag gagctgtgtt agctgttgtg gcgttgtggt
      901 tacgtcgtca gttagatgaa acttcgcaac aagaaacgcg cgctttaaaa gaagctggat
      961 ctctgaaagg attatggcgc aatcgccgtg cattcatcat ggttctcggt tttaccgctg
     1021 cgggctccct ttgtttctat accttcacta cttatatgca gaagtatctg gtaaatactg
     1081 cgggaatgca tgtcaacgtg gcgagtggca ttatgactgc cgcattgttt gtattcatgc
     1141 ttattcaacc actcattggc gcgctgtcgg ataagattgg tcgccgtacc tcaatgttat
     1201 gtttcggttc gctggcagcc atttttaccg ttcctattct ctcagcattg caaaacgttt
     1261 cctcgcctta tgccgctttt ggtctggtga tgtgtgccct gctgatagtg agtttttata
     1321 catcaatcag tggaatactg aaggctgaga tgttcccggc acaggttcgc gcattaggcg
     1381 ttggcctgtc atatgcggtc gctaatgcta tatttggtgg ttcggcggag tacgtagcgt
     1441 tgtcgctgaa atcaatagga atggaaacag ccttcttctg gtatgtgacc ttgatggccg
     1501 tggtggcgtt tctggtttct ttgatgctac atcgcaaagg gaaggggatg cgtctttagt
     1561 gacgggtcag ttgccagacg gtatagccgg tgcttgcacc ggcgacatcc caggccaaat
     1621 ccttccagct ccagccgctc ccttcggggc ggctatccca aagctctttt gacgccccca
     1681 aactgacaga gaacatcaat ccaaacatgg cactgcgatc ccggctcatc ccctgatgct
     1741 gtgaatattc atttccggcg gcggaaagca tcgccgaggc gataaagtgt tgagctttat
     1801 cctgcccgct ccagctatcg ttagccatat ggctgcatcc agaaagtaac aatagcgaac
     1861 agacaaaaag aatacgcaaa acgtggcctc caaaaaacaa accccgtaca ggacggggtt
     1921 gtgattacag gatgcggcta attaatcggt cgatgcggat acggcgcaaa cggcggatga
     1981 gtttacgaac cttcaccgga taatcggcaa tactttgcag atcgcgatag tgcttaacga
     2041 tggtggtatg ctcgcggatc agctccagtt ctttctctcg ctgcggcgca agcttcagtt
     2101 gtggatcgtg gatcaaaatg gcgttttcca gatccagacg ccaggcgcgc gggttcaggt
     2161 tattaccggt gatcaacatc cacttatcat caacccacat ccctttcagg tgataggtgt
     2221 tgtcgtcatc tttccataac cgaaccacta gctggtcagt attgacgtaa tactgcaaac
     2281 ggctcaggaa acgacgcaga ttgatctcat agagataagg caatgcgcca attatcttga
     2341 aaggttcatc ttccggaatg tagaagtcgt tcgcggtttt atcaccaaca ataatttcga
     2401 cctttttccc ttcgcgcagc aactggataa tattgcgcac aaggattgct ggcaggttga
     2461 agtatggcgt acagatggtt agtttctgct cggcacaagg cataagatgg aaaatggtct
     2521 tgttcaacag actcgatttc cccagcccca ctagcggcgt tacggaaagc tgatcgttgt
     2581 cggcatcgcc ctggaaatga taagcggcat cacgcagctc ctggcggaac agacgaatat
     2641 cgttcttgat ttccgggctt tttggccgat taacatcatc cagacgatta acgccgcggc
     2701 cattcataat attctgtgta acccattcaa acataatgtc tgacatctta cggttacgga
     2761 taagatgata acggtcgtag cgatatttat cgtgctgatg caggtaaaca tcgttcaggc
     2821 tggcaccgct ataaagtacg ctatcgtcga tgataaagcc tttaaagtgc agaacaccaa
     2881 gggcttcacg ggtattgatt ggaacgccat aaaccggaac atctacgccc gaattttcct
     2941 gcgccatgcg gcagtaccag tcagcgttag tgttagatgc cgcagcgcca atgcgtccac
     3001 gttgtgcacg atgccagtcg accagcaccc gcacatccag ttccggacgc tgccttttag
     3061 cttcatacaa cgcgttcaga atgcctttgc caccgtcatc ctgttcgaga tacagggcga
     3121 caatgcaaat gcgctgcttc gcgctggcta ttttttccag cagcgtctcc cggaagtcgg
     3181 cgggagcgta aaagaaatcg acatcatcaa ctgattgaga aatcttgggt agttgggcaa
     3241 ggtgttgttg atgtttatta cgcttaaatt ttgacaacat cacagtgcat ttcttctctg
     3301 ttcattgaag ggtcctctgt gcaatgcaga cgacataagc gggcaataat aacaccagtc
     3361 ccgattaagt ggtcaacatt tccagtacct tactcatgat tcctcgcgtt gggcaagatt
     3421 tagcgtaagc ccaacgatcc cctcttcgag ctggatatca acgttaaacc cgagcttgcg
     3481 ggctagcgcc accatgccac gattgtttgg catcgtaata ccattcagac gttgtagtcc
     3541 gtgatctcgc gtataggtaa tcaacttttc cattaagcgt cgacctaagc ctaacccttt
     3601 gagatccgag cgaaccagca cagcaaattc ggcatcgatg ttatcaggat cggaaatcgc
     3661 acgcgtgacg ccgaggatct cttccgtttg atcaatacgt cgtaccgcta caaacgccat
     3721 ttcccgatcg tagtcgatct gtgtcatgtt ggctaaatct tcatgggtaa attcgttgat
     3781 ctcgctaaag tagcggtaat aaagatcttc tttggtgact cgcgaaatga attgctgaag
     3841 ttgtggctca tcttctggca aaatcgggcg gaacaagcag cgttcaccgt ttttcaattc
     3901 tacccattct tccagctgat gcggataagg gcgcactgcc agccgactct cgttatcgcc
     3961 ttcaaacggc gagatatcca gcgtgacatc cagcgcggta aattcactgc cagaagccag
     4021 caaaggatga atatccagac gctgaatttc cgggcaatcg acaatcaagt tggaaacctg
     4081 caccagaagc tggctcaagc ctgcaacatc caatgggcgt agcgcactgc gcgcacgaat
     4141 ctttttactt ttgatcccct gaataaccag atagcgggcc aggttcatgt tcagcggcgg
     4201 cagtgcgacg acggcttgat cttcaggacg ccactccaca ccgccttcac ccagcatgat
     4261 caacggcccg aaaaccggat cgtgctcaac cacaacccgc aactcctgag cgccagcacg
     4321 gttagccata ctttgcacca acaggccgtg gacccgcgcc tgtggccagg ccatttttac
     4381 gcgatcgaaa atagcgttcg ccgcttgctg gacttcattg gctgtacgca ggtaaagcat
     4441 gacgccctga acttccgatt tatgtggaat atccggcgaa cgcaatttca gcgccaccgg
     4501 ataaccaatc tgttcggcaa tatgcaccgc ttcggtgcta tcgctggcaa tccaggtagg
     4561 gagcgtgttc atgccatacg cttgcaggat gggctgaact tcatgggtat cgagcgacgt
     4621 agccccttcg gcaatcgcct gttgcaacag aagatgcgct tctgcggtat tggaagtcag
     4681 attgctgggc aacgtcggcg tttcgcgtag ttgcttctga ttacgccggt actccaccat
     4741 atgcataaaa gcagtgatgg ttccttccgg ggtacggtag gtcggcagcc cggcttcgct
     4801 gaataaacgt cgtgcctctt gcgaggagtg ctcgccgcac cagttcgtca gcaaagagac
     4861 atatttgctg cggggatgat gctttaccgc ttcaattaat acttgcgcgc tttctgttgc
     4921 gggagcagcg gcgctgggcg aatgaataac catcagcgca tcaaaatcct ggctgtggag
     4981 cagaatgtcc agcgttttga cataatgctc gctgctggcg tcatcgcgca gatcgagagg
     5041 gttagagact gccacatgtt ctggcaatgc atcgcgcaat ttctggcagg tttcttcgct
     5101 tagcgttgcc agcttgccat tgcgtgacca taaggcatcc agcgccagcg cggcaggcgc
     5161 agcaccgttg ctgataatca tcaaccggtc gccacgcagc gggcgcatat ggctaagggt
     5221 ttccaccgcc gaaaacagtt cgtgggtatc ctgtacccgc aacaaaccgg cacgctgaat
     5281 agccgcatcc catgccgggt ccattcctgc cgtcgtgttg agcagtcgct gtgccgccgg
     5341 gctacgtccg cttttaatca ccagaatcgg tttattacgc gaggcactac gggctgccga
     5401 aacaaagcgt cgcgcgtcgc ttaactgttc gagatagagc aggatggcgc tggttttact
     5461 gtcgcgcgcc agatagtcga gcaattcatc aacgtcgata tccaggctgt cgccgagcgc
     5521 aataaagtag gaaaagccca tctcacgctg ttgcgcccag tcgaggatgg tgttggagac
     5581 ggctgccgat tgtgaaataa acgccagctt gccgcgttta atcggcacag gcgaaaagct
     5641 ggcattcaga ccttgccagg gagccagtaa acccagactg tttggtccaa gcaggcgcat
     5701 gttatggcgc agggcgcagg cgcggagatc ttcgtgttgc gatgccgggg cggaaagaat
     5761 aatgcaggtt ttacagcctt tctcgccgag ctcttccaga agagcaagat tacggctggc
     5821 attggtacat aaaaccgcaa ggtcgggtgt aaagggcaag ctggcaatat ccggccaggc
     5881 caacacaccc aacaccgctt tccaggctgg cgtcaccggg agtaccggtc cgttaaagct
     5941 tcccgccagc aggttacgca tcatcaggta acctgcgcga ttgggtttca tcgacgcgcc
     6001 aattaccgct atcgattttg gtcgcagtag tgcttccagt cctcgctgac tcataccggt
     6061 ctcccgcaga agtgaccgaa tgattttaaa cgctttctaa ctgttctgct gtgatgctac
     6121 ccagatgttg cgtttttcct gccagatagc gtgttttaaa gcgggtaaaa tgctcgccta
     6181 accctgctgc cgccccggta tcgccggcca tatctaacag tgcgatggct acctcggcag
     6241 tacaatattg gcctgcagcc tgggcttcac gcaggcgata ggcagaaagc cgggaaagat
     6301 cgacggaaat gacgggaaga ttatccagat acggactttt acgaaacatc ttgcgagctt
     6361 ccggccaggt accatcgagc atgataaaca gcggtggctt accggcaggt ggtgtgaaga
     6421 tcacttcccg ttgctcatca gcatacgagg cgggaaagac caccattggc tgataatccg
     6481 ggttttgtac cagatccagc aaatcctgcg agggttcggt acgcgaccat tgaaacgcaa
     6541 cggtatcagg caaaatatca gcaatcagac gcccggtatt actgggcttc attggctcgg
     6601 tgtcgaacat cagcaaacag aagcgacttt ttgcttgtgc tggggtaatt gtcgaacaga
     6661 gacataattt ctctggcaaa agacagcgtt ggcagcgatg aacgcgatta ccgcgggcaa
     6721 gaaaaggacg tgttgcgcgc gcaatacgct cggcgcgtaa ctggagaaca gcgttttcgg
     6781 tcataagaga gcgtcgaaaa aacgccattg tcgcagagga gaaaacgggg cacaagatgc
     6841 accccggtaa gattaaagag attcgttcag ccagctatcg aacggcgctt tcggtactgc
     6901 gccattaagc atgtcgacaa cctgaccgtt tttgaaaatc atgatcgtcg gtatactacg
     6961 aattccaaag cgactgctca attcacgttc agcttcggta ttcactttca caaagcgcac
     7021 tttaccgcta cgctcttgcg cgacatcttc aaaaattggt gcgaaattac ggcaggggcc
     7081 gcaccacggt gcccagaagt cgatcaccac aggtagatca tccttcagca atttgtcgag
     7141 tgtttcaccg gtcgcattaa tcacctctcc gtcaaacaag tcgtgaccgc agcgtccgca
     7201 ttttgccgca tcttcgatcc gatcgtcggg aatgcgattg atggcctgac aatgggtaca
     7261 aacggtattc ataactaacc tcgggatgag taaggagact tatcggcaac gacgccggta
     7321 tgtttctaat atgttacata ttatcgggga cattgttcca gacaacaatc tgttttattc
     7381 aataggtatg atagtcgcag gctttggtac gaaatcatgg cgaaccgcgc ggacttcggg
     7441 taatctgcgc gcttcgcgca gcgctggtgg agaaaagcat gaacgatgaa atgaaaggta
     7501 aaagcggcaa ggtcaaagtg atgtatgtcc gcagtgatga tgattctgat aaacgtaccc
     7561 acaacccgcg taccgggaaa gggggcgggc gtccaggaaa atctcgtgct gacggtggcc
     7621 gtcgccccgc ccgcgatgac aaacagagtc agccccgtga ccgcaagtgg gaagattcgc
     7681 cgtggcgcac ggtttcccgc gcgccgggtg atgagacgcc ggaaaaggcc gatcacggtg
     7741 gcatcagtgg taaaagtttt attgatccgg aagtgttgcg tcgtcagcgt gcggaagaaa
     7801 cccgcgtcta cggcgaaaat gcctgtcagg cactgttcca gagccgtccg gaagcaattg
     7861 ttcgcgcctg gtttatccag agcgtaacgc cacgttttaa agaagccttg cgctggatgg
     7921 cggcaaaccg caaagcgtac catgtggtgg atgaagctga attgacaaaa gcgtcaggca
     7981 cggaacatca cggcggcgtt tgcttcctga tcaaaaagcg taacggtaca accgtgcagc
     8041 agtgggtaag ccaggcaggc gcgcaggatt gcgttctggc actggaaaat gaatctaacc
     8101 cgcataacct gggtggcatg atgcgcagct gcgcgcactt cggcgtgaaa ggtgtcgtgg
     8161 tgcaggatgc ggcactgctg gagtcggggg cggctatccg taccgcagaa ggcggcgcag
     8221 agcacgttca gccgattact ggcgacaaca ttgttaacgt gctggatgat ttccgtcagg
     8281 caggttacac cgtagtgacc acttccagcg agcagggtaa accgctgttc aaaactagtc
     8341 tgccagcgaa aatggtactg gtgctgggtc aggaatatga agggttaccg gatgccgcac
     8401 gcgatccgaa cgatctgcgc gtgaagattg acggtacggg caacgttgcc gggctgaata
     8461 tctctgtcgc aaccggcgtt ttgcttggtg aatggtggcg tcagaataaa gcctgataaa
     8521 tcagccgggt ggcaactctg ccatccggca tttccccgca aatttactca ctctctgccg
     8581 gtaatactgg catccagtca atcggcgtct cgccacgttg ttccagccac tgatttgcca
     8641 gcacaaaatg gttgcagcca aagaatccac gatgcgccga aagcggcgac ggatgcggtg
     8701 ctttcagtac atgatggcgt tgcttatcta taatcgcccc tttcttttgc gcatgtgatc
     8761 cccacaacaa aaacaccacg ccttcgcggt gctggttaat caggctgatg actttatcag
     8821 taaacgtctc ccaaccgagg ctggcgtggg aatgcgcctg acctgcgcga accgtcaaca
     8881 cagtattgag cagcagaacg ccctgacgcg cccagctttc gagatagcca tgattagggc
     8941 gggtgaagcc cggaatagta ttttccagct ctttatacat attcaataat gacggaggaa
     9001 tggcaatgcc gggacgaacg gaaaatgcca gaccatgcgc ctgtcccggt ccgtgataag
     9061 gatcctggcc gagaatcacc actttaacgt cacccaactc ggtaaagcgg aacgcgttaa
     9121 agacatcttt ttgtggtgga tagatagtga cgccggactg ccgctcgctg gcgacggtct
     9181 gaagggtatt aagaaaataa ggttgttgct tctcttcagc cagcacgtca tgccaggtta
     9241 attcgttagc catctcgctc tcctgcgaat cttcaatccg cctagcttaa ctgcttcttt
     9301 ctacagaaca aaatcatgct cattttttag acttaaacga gtgcaaaaaa atgtaaattg
     9361 gtaaaatttc acacccgcat aagttgatgt aaaacaataa aatccattta cgaccacaat
     9421 cgatgtgtgg tttttattga tttaaatcaa agaatcaagg gtggttgagg agtatatata
     9481 cactcaagca acaatggttt taccaattgg ccgcgacagg ctgaacaaat caaataattt
     9541 tgccggggag gcatcacatg attacaggta tccagattac taaagccgct aacgacgatc
     9601 tgctgaactc tttctggctg ctggacagcg aaaaaggcga agcacgttgc atcgttgcaa
     9661 aagcaggtta tgcagaagat gaagtggttg cagtaagcaa actgggtgac attgaatacc
     9721 gtgaagttcc agtagaagtg aaaccagaag ttcgcgttga aggtggtcaa cacctgaacg
     9781 ttaacgttct gcgtcgtgaa actctggaag atgcagttaa gcatccggaa aaatatccgc
     9841 agctgaccat ccgtgtatcc ggttatgcag ttcgctttaa ctctctgact ccggaacagc
     9901 agcgcgacgt tatcgctcgt acctttactg aaagcctgta aggcttaagt agcaagttac
     9961 taagcggaag agggcgacct cttccgcttt ttttcgttaa tagaaaatgc gtaccgcgca
    10021 atagaccagt aacagggcaa gcacgatatt taactggcga ccatactggc gaaacaatcg
    10081 ctgaaacaga tgccccgcca gcgcccagca cacattgcca aacgtcccaa tcatcgccag
    10141 caaaacgctg acgccaacta cccagcttag cgcctgtgtt tgcggcagaa caaacgtcga
    10201 cagtgccgta acaccgtaca aaatgatttt gacgttcaca aactgcaaag caaagctggc
    10261 ccaaaagctg attggttttg cctgaagtcc gtcttccttt gttgggctgg tggcgatttt
    10321 ccacgccagc cagacaatat atgccgcccc cgcccaactc aaaaggtgta ccgctgccgg
    10381 gtcaatcact gccagtgaaa atgaaatgcc cgcacacagt aacatcacaa tcaaaaatcc
    10441 cagactcatc cctgccagca cgcgggtact ttgacgaaat ccatgcgacg tagcagagct
    10501 aagggcgaga atattgttcg gtcctggcgt catagcggta atcagggtgt aagtccaaaa
    10561 agcacttaaa agggtcggtg tcacttgtta tctcctctgt tttggtagac agtacccgac
    10621 acggaacatt taaaaaaatg aatgttttta tattctagat tcgatatttt cgataggttt
    10681 ggggttatgg atctgcgccg ttttattacg cttaaaaccg tggtggaaga gggttccttt
    10741 ttgcgagctt cgcaaaaatt gtgctgtaca caatcgacgg tgacttttca tattcagcag
    10801 cttgagcagg agttttcagt ccagttattt gagaaaattg gccgacggat gtgccttacc
    10861 cgcgaaggaa aaaagttgct gccgcacatt tatgaactta cccgagtgat ggatacgctt
    10921 cgtgaggctg ccaaaaagga gtcagatccg gacggggaac tacgcgttgt ttcgggcgaa
    10981 acgttgctct cttaccgaat gccgcaggtg ttgcagcgtt ttcggcaacg cgcaccaaaa
    11041 gtgcgtttat cgttgcagtc gctgaactgt tacgtgatcc gtgatgcgct actgaatgat
    11101 gaggccgatg tcggcgtctt ttatcgcgta gggaatgatg atgccctgaa ccgacgagag
    11161 ttgggtgaac aatcgctggt gctggtggct tcaccacaaa ttgtagatgt cgattttact
    11221 gagccgggaa gacataacgc ctgtagcttt attatcaatg aaccacaatg tgtcttccgg
    11281 cagatatttg agagcacgct gcgtcagcgg cggatcacgg tggaaaacac cattgagctt
    11341 ataagtatcg aaagcatcaa gcgttgtgtc gcggcgaata tcggagtcag ctacctgccg
    11401 cgttttgcgg tggcaaaaga gttggagtgc ggagaattaa ttgaattgcc ttttggcgaa
    11461 cagtcgcaga ccattacggc catgtgcgca caccatgctg gaaaagcggt tagcccggcg
    11521 atgcacactt ttattcagtg tgttgaagag agttttgtgg ctggataaaa ataatgtcgg
    11581 gattaaaccc ggcatttaac ttgtcgacaa agtgcgcttt attcatgccg gatgcggcgt
    11641 gaacgcctta tccggcctac aagatcgtgc aaattcaata tattgcatga gccacgtagg
    11701 cctgataagc gtagcgcatc aggcagtttt gcgtttgcca gcacttttaa taccaggctt
    11761 aaacccggca ttgagattac tcttctgtcg tttgcggtgg cacgcccgtt ccgcttggtt
    11821 tacggcgctt accaatattt ttggtgtcgc gatggcgttt tttcacccgc ggtttctctt
    11881 tctctttagc ttttttcttc tcggcgcgtt ttgccagtac tttcttcgat ggcttgccgg
    11941 tctgcttctc gcttggcgca cgcgttttcg ggcgtaactc atcaataacg cgagctttaa
    12001 tcggctcttc aatgtaacgg ccaaccttgc ccagcagcaa atggtcatgg gcttccacca
    12061 gcgaaattgc ggtgccttta cgaccggcgc gcgcggtacg tccgatacgg tgcaaataag
    12121 tatcgccact gcgcggcata tcgaagttaa agacgtggct gacgtcagga atgtcgatac
    12181 cgcgcgcggc aacatcggtt gcaaccagta cgtttacgcg accttcggtc aaacgcttga
    12241 tcgcttcgtt acgcttgcct tgtaccatct caccttcgag atagcagttg ttgatgcccg
    12301 cttcgcgcag ccagtttgcc agctcatgca cacgttcacg cttacgcaca aacacaattg
    12361 agcgggtcgc ttccggctgt tttaacagat gcaccagcaa tgcggtttta tgttccaggt
    12421 catcggcgcg gtaataccac tgatgaattt ttttgcgctc acgggtggac ggattggcag
    12481 aaacctccac cggatcttcc agcagacgct cggcaaagtc ctgaatcgca tcgccttcca
    12541 gcgttgccga aaagagcagg gtctgtttac gccagcgcgt ttcgccagca atatgttcga
    12601 tatcctgagc gaagcccata tccagcatac ggtctgcttc gtcgaggatc agcgtttcaa
    12661 ccgcgcggca atcgaagttc tcttctttta tgtattgcag cagacgtccg gtcgtggcga
    12721 ccacgatgtc ctgattttcg ctgaacactt ccgcgtggtt catataggct acgccgccgg
    12781 tgatggtggc gatatccaga tgcgtatgtt tcgccagttc gcgggcatga tcggccacct
    12841 gcatcgccag ctcgcgagtt ggggtgagga tcaaaatacg cggcggaccg gatttcttac
    12901 gtgggaaatc gagcaggtgc tgcaacgctg gcagcagata cgccgccgtt ttaccggtgc
    12961 ctgtcggcgc agaaccgagt acatcacggc catcgagcgc aggcggaatg gcggcagcct
    13021 gaatggcggt cgggcgagtg aaacctttat cctggagggc ttccagcagg ctttcgtcga
    13081 gttcaagttc ggaaaaagtc gttacagtca tgttctacct ctgtgtgggg cgctgattat
    13141 agacgttacg gctgcaatct tcatctgttt gtatggatat cgcttctcgg gtattgttct
    13201 cacccggtgc tatcgccgtt tttcctgcaa ggttttattt tcatgtcaca gtctacatcc
    13261 gtgcttcgtc gtaatggatt tacttttaaa cagttttttg ttgctcacga tcgctgtgcg
    13321 atgaaagtgg gaacggatgg cattttgctg ggcgcatggg caccggtggc tggggtgaaa
    13381 cgttgccttg atatcggcgc gggtagcggg ttgctggcac tgatgctggc gcagcgaacc
    13441 agtgacagcg tgataattga tgccgttgag ctggaaagtg aagctgccgc gcaggcgcag
    13501 gaaaatatca accagtcccc gtgggcagag cggattaacg tccatacggc ggatattcag
    13561 cagtggctca cacagcagac agtacgcttc gatttaatca tcagtaaccc accttactat
    13621 cagcagggag tagagtgcgc gacacctcaa cgggaacagg ctcgctatac cactacgctt
    13681 gatcacccat cgctgctgac ctgtgcggcg gagtgcatta ccgaagaggg atttttctgc
    13741 gtggtgctgc cagagcaggt tggtaatggt tttactgaac tggcattaag catgggctgg
    13801 catttacgtt tgcggacgga tgtggcggaa aacgaagcgc gactgccgca tcgggtgctg
    13861 ctggcattct ccccgcaggc gggagaatgc tttagcgatc gcttagtcat tcgtgggcca
    13921 gaccagaact attccgaagc gtatacggcg ctgacccagg ctttttatct gtttatgtaa
    13981 tgattgccgg ggggaaggat cgatggaccg gaatgcgtga gcagttccgg gtaatccagc
    14041 gtaaaatgca ggccacgact ctccttgcgc atcatcgcac agcgaacaat caactcggca
    14101 acctgtacca gattacgcag ctccagcaaa ttattcgaca cgcggaagtg ggcgtaatat
    14161 tcgtctattt cctgttgcaa catattgata cgccgcaggg cgcgctcaag acgttttgtt
    14221 gtgcgcacaa tgccgacgta atcccacata aacagccgta gctcgtgcca gttatgctga
    14281 attactaccc gttcgtcagg gttctcaacg cggctttcat cccacggtgg taacgtactg
    14341 acgccgtggg catcaggcat acgtctggtg atatcttctg ccgccgacca gccatagacc
    14401 agacactcca gcaatgaatt cgaggccatg cggttagcgc cgtgtaagcc ggtataactc
    14461 acctcgccaa tggcatacaa gccctcgacg tccgtacgcc catgatcatc aaccattaca
    14521 ccaccgcagg tatagtgtgc agcaggcaca atcggtaccg gttcttgtgt gagatcaatc
    14581 cccagcccga gcaatttttc ataaatcatc gggaaatgct ggcgaataaa atcggcgggc
    14641 ttatggctga tgtcgaggaa catacaatct gcgccgaggc gtttcatttc atggtcaatg
    14701 gcgcgggcga caatatcgcg cggggccagt tcgccgcgct catcaaaatc gggcataaaa
    14761 cgcgtgccat ccgggcgctt gagataagcg ccttcgccgc gcagtgcttc cgttaacagg
    14821 aaattgcgtg cctgtgggtg atacagtgcg gtagggggga actgattaaa ttcgagattg
    14881 gcaacc
//
LOCUS       DAZPDU010000109        14334 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZPDU010000109 DAZPDU010000000
VERSION     DAZPDU010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14334
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.110"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            71..427
                     /locus_tag="ACHURS_002920"
     CDS             71..427
                     /locus_tag="ACHURS_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR1238596.1"
                     /translation="MQWYLSVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLELPYLSMLYLLATFLPVLALAIRRLHDTDRSGAWALLFFVPFIGWLVLLVFFCTEG
                     TSGSNRYGNDPKFGSN"
     gene            complement(478..1370)
                     /gene="yhaJ"
                     /locus_tag="ACHURS_002921"
                     /pseudo
     CDS             complement(478..1370)
                     /gene="yhaJ"
                     /locus_tag="ACHURS_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
     gene            1475..2176
                     /locus_tag="ACHURS_002922"
     CDS             1475..2176
                     /locus_tag="ACHURS_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFR1238597.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            2199..2363
                     /gene="yhaL"
                     /locus_tag="ACHURS_002923"
     CDS             2199..2363
                     /gene="yhaL"
                     /locus_tag="ACHURS_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFR1238598.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2497..3807)
                     /gene="cyuA"
                     /locus_tag="ACHURS_002924"
     CDS             complement(2497..3807)
                     /gene="cyuA"
                     /locus_tag="ACHURS_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFR1238599.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3835..5166)
                     /locus_tag="ACHURS_002925"
     CDS             complement(3835..5166)
                     /locus_tag="ACHURS_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR1238600.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGAFYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(5441..6805)
                     /gene="tdcG"
                     /locus_tag="ACHURS_002926"
     CDS             complement(5441..6805)
                     /gene="tdcG"
                     /locus_tag="ACHURS_002926"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFR1238601.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAAWMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6877..7266)
                     /locus_tag="ACHURS_002927"
     CDS             complement(6877..7266)
                     /locus_tag="ACHURS_002927"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFR1238602.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(7280..9574)
                     /gene="tdcE"
                     /locus_tag="ACHURS_002928"
     CDS             complement(7280..9574)
                     /gene="tdcE"
                     /locus_tag="ACHURS_002928"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFR1238603.1"
                     /translation="MKIDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9608..10816)
                     /gene="tdcD"
                     /locus_tag="ACHURS_002929"
     CDS             complement(9608..10816)
                     /gene="tdcD"
                     /locus_tag="ACHURS_002929"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFR1238604.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVVIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10842..12173)
                     /gene="tdcC"
                     /locus_tag="ACHURS_002930"
     CDS             complement(10842..12173)
                     /gene="tdcC"
                     /locus_tag="ACHURS_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFR1238605.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(12195..13184)
                     /gene="tdcB"
                     /locus_tag="ACHURS_002931"
     CDS             complement(12195..13184)
                     /gene="tdcB"
                     /locus_tag="ACHURS_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFR1238606.1"
                     /translation="MHITYDLPIAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(13283..14221)
                     /gene="tdcA"
                     /locus_tag="ACHURS_002932"
     CDS             complement(13283..14221)
                     /gene="tdcA"
                     /locus_tag="ACHURS_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFR1238607.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         3701 a         3845 c         3348 g         3440 t
ORIGIN      
        1 aaagatacga atagcattta cacaataccc ggaatagact ttcgtcactt ttaattaaag
       61 ggatgttttt atgcagtggt atttatccgt attaaaaaat tatgttggtt tctctggtcg
      121 cgcacgtcgt aaagagtact ggatgtttac tctgattaac gccatcgtcg gcgctattat
      181 caatgtcatt caattgattt taggtctgga gcttccatat ctgtctatgc tttatttgct
      241 ggcaaccttc cttcccgtcc ttgcgcttgc tatccgtcgc ttacacgata ccgaccgttc
      301 aggtgcctgg gccttgctct tttttgttcc gtttattggc tggctcgtgc ttctggtttt
      361 tttctgcaca gaaggtactt ctggcagcaa tcgttacgga aacgatccga agtttggttc
      421 aaattaatct tagaattggg gcgatatttc gccccttttt attaacaata ataaatatta
      481 ttttccgcta aaaagtttgg gaatttcccg cagacaccag gatttcgctt cccccatact
      541 gtcacgacgc caggccataa taatatcgat ctcactggtc gattccgggc tgacgacacg
      601 caaccgccct tccgcaatat ctttttcgac catcggatac ggcatcgtcg ccacgccaag
      661 ccccgccagt aatgcctgac gtttatcttc aatcgtgctc accgttaagc gcggctgttt
      721 gtccagcagt tgtacggtca acaccgggcg ctcacgagcg gtatccgcta ccgcaattcc
      781 acgatatttc acgcgcgtca cttcagataa cggttccggc tcctgatgaa tcgggtgatc
      841 aggcgcggca acgtagacgt tcattaacgt atagagcttg cgcgagttga tctccgacga
      901 ggaacgaaaa tgcatatccg gcgcgataac aatatccgcc cgcccctgct ccagtcgttc
      961 ctacgccccc gccagcactt cggtgatgat tgccagttgg gtattggctt ttgccgccag
     1021 tttgtcgatt aacgggaaaa aggcaggtgt cggtaccagc gcttcggtca caatggtgag
     1081 atgcgtttcc caaccgcgcg cgagggcttc cgcatcggta gtcagtttat ctgcggcttc
     1141 cagcaaaacg cgccctcgct ccagcaacat cctcccgaca ttggtgaatt tggtgcgatg
     1201 gcccgagcgg tcaaacagca ccacatccag ctcttcttcc agtttttgca tggtgtagct
     1261 aagtgcggaa ggcacgcgtc ccagctcatc cgccgccgcc gcaaaactgc cccggcgatc
     1321 gcatccataa cccgtagtgc ttccagcgtt aatgcccttt ctttggccat ttcgttctca
     1381 ttcaggaaat ttgaacatac cgggcagaat atctggctaa caatgtagcg tccagcccct
     1441 taccataaaa ggaagtaaag agaggtcaag aattatgatt actacccgaa ctgccaggca
     1501 gtgtggacaa gcagactacg gatggttgca ggcccggtat actttttcct ttggacacta
     1561 cttcgacccg aaattgttag gctatgcctc cctgcgtgtg cttaaccagg aagtgctggc
     1621 cccaggtgcc gcctttcagc cgcgaaccta tcccaaagtc gatattttaa atgtgattct
     1681 ggatggagaa gcagagtatc gcgacagcga aggcaatcat gttcaggcca gcgccggtga
     1741 ggcgttgctg ctctctaccc agccgggtgt cagctacagc gaacacaatc tcagcaaaga
     1801 caaaccgtta acacgaatgc agctttggct ggacgcctgc ccgcagcgag agaatccgct
     1861 gattcaaaaa ctggcgctta atatgggcaa gcagcaatta atcgcctcgc cagagggtac
     1921 gatgggaagc ctgcaattac gccagcaagt gtggctgcac catatcgtgc tcgacaaagg
     1981 cgaaagtgcg aatttccagt tgcatgggcc acgcgcgtat ttgcaatcga ttcacgggaa
     2041 atttcatgcg cttacgcatc atgaagagaa agcggcgctg acctgcggtg atggggcgtt
     2101 tattcgtgac gaggctaaca ttacgctggt tgccgattcc ccactgcgcg ctttgctgat
     2161 agatttgcct gtctagttgt ttttacaggg agatgatgat gagtaaaaaa tcggccaaaa
     2221 agcgccagcc agtgaagccc gtggtggcga aagaacctgc tcgcaccgcc aaaaattttg
     2281 gctatgaaga gatgttgagc gagctggaag ccatcgtcgc ggatgctgaa acgcgtttag
     2341 ccgaggatga agctaccgcg taaagacagg ttgccggatg cggcgaaaac gccttatccg
     2401 gcctacaaaa tccaacaaca ttaacgaatt aaacaacccg taggccggat aaggtgcgcc
     2461 agcatcgcat ccggcacaat actcaaaacc tggcgtttat ctggccttgc tcgccataat
     2521 ctcgataatc tgccgatccg tttgctgcat cgaatggctt gctaacgcac acaggttggc
     2581 aatcgactgc tcaacatcat gcgccacgat cccttcattg ccggtcacgg cggtatcatc
     2641 cagcgccatt aacaccgctt tccacgcagc cgaagcactg gtcgaaacct tcatcgcgca
     2701 gctgttcgac gcaccatcgc aaatcatgcc gctgacatcg ccgatcatac tgctgatcgc
     2761 catcgagata gtttcataac gcccatccac cagccatgcc atcccggcgg cggcccccat
     2821 tgctgcggtc gttgcggcac aaagtgcaga caaacgcggt aactggttat ggatgtaaat
     2881 tgcgctcaaa tgagaaagca tcagcgcacg cgccagtcgt tcatcatccg ctccgaagtg
     2941 ttctgctaca accaccacag gcattgttgc ggtgatcccc tggttacccg agccggagtt
     3001 actcatagcc ggaagcgtag cgccgcccat acgcgcatcg gatgccgcgc tggtacgaat
     3061 cacaatggat gaagagagat ctttcgccag caaaccgcgc tcgcactgtt tttccagcgt
     3121 cgcgccaata tgcagccccc acttaccgct caaaccttcc tgcgataacg cacaatttag
     3181 cttcgcggaa tcgagaataa agcggatcgc cgcaaacggg acttcattga cgaacttcag
     3241 gatctcagcc agcgtcgttc tggaaagcac tgtcagcgga gactcttgct cgccctctgc
     3301 cacacacgcc tgctgggtaa acaccacacc attgtgcgtc tcaatatgca caatgttggt
     3361 atgcccgccg acgatggtga cacacgccca cttctcaccg ttccagactt tggcgcgtga
     3421 gaagaggatt tcattgcaag gttcctggat cttaacggag actttccccg ccgccagcag
     3481 tgctttggca tcggcaattg cctgcgcagt tgcgtctttc agcacttcca gcccggcgtt
     3541 ggcatttcca cctaacgccc ccagcgccgc cgcaatcggc agccccacca ttcccgtgcc
     3601 gggaacggtg acgcccagac cgttcttcat cagatttggc gaaacccagg cttctacacg
     3661 ttcaaccgga ccttccagtt ctgccgcagc aaccgccgcc gccagcgcca gtgaaatcgg
     3721 ttcagtacat cccagcgccg gttttacttc ctcctgaacg gcgaggatgt aacgctgcca
     3781 taacggattt aaagtcgaat caaacatatt aaaaacctta aaatttcagg taaatcaaga
     3841 aaatgccagg aacggagaaa cacaaagcaa caaaccagtg acgataatca ggtacagaga
     3901 catccctttg tatttgtgca atgccggtac tttgtaaacc agccacgccg ggatcaggca
     3961 ccctaccatg ccgaaaatcg ggctacagat agaggtgaag ctcaacaccg gtgcgttcag
     4021 tacgatagcg ctccaggcca gcaaaatggc gaaaatcatg atgccgcgct gaacaagatt
     4081 ttcgttaatc ttctcggcag gcatcttgcg acgcaggatg ttcattacga tcccttgcgt
     4141 tgcttcgcga aagcctaaat agacgccaaa gaatgcagtc atgacagcaa agatattgag
     4201 aatgacgctg accactttca cccacgctgc gccgtcaccg ctaataaact gtgcggcaat
     4261 cgccagcgcg gaaatattct gctcgtaggc ttttaccgct tcgtcatgcc ccatcgccag
     4321 cgtgaacgac acggcgtaga aaaagacggt gacaaacaaa atgccaaacg cgatattcat
     4381 tgcccgcaat gctttatgcc gcgccacttc aatagatttt tcccgcgagc gataagagat
     4441 caccatcggg cttaacgtct ggataaacaa aatcgacgtc agggtaaacg gcagcgtaat
     4501 aatggcgttt ttcaccagca gccccagcgg cggtagcgaa ccgacgttat acagatgcca
     4561 cattcccacc atcgacacgc ccagcgccgc gaccaccagc agcttggtca gcaccatgcc
     4621 ggtcgaaatt ttgaataaca atttctcgcc gcgtgaagag atcgccacca gaatgcaaat
     4681 cagtaccaga ccataaaagg gactgtctga cagcaaccct tccgtcacgc cgaaggtatg
     4741 cagataggaa gcactatcgt tggtgatggc ggtggaatag acgaacatcc aaatcaccag
     4801 cattacgaaa tagaacgcac ctaacaggat gccccagttt ttacctaaat aaccgctaat
     4861 gacactcggg taatctttac attctggtga ttctgccagc gtattaataa acaaccgctg
     4921 aaacagatac attgccgggt aaccaatcac cgatgagagc aaaaataccc acaatcccat
     4981 caaaccgacc tgcaccggga gaaaaacaat ccccgcgcca atcgccatcc cgatactcat
     5041 aatcacccag ccggtgtcag tactgtcgaa tttgatcgct tctcgccact cgctctcact
     5101 cattccagca cgacccgccg gggtcgaagc gtctgcaatg acgcctttat tcgatgcaat
     5161 ttccataatt tctcgctcaa tattttgtag ggcttattat ttttttccga gccgcatcaa
     5221 ggcgatatgc ggtctacgtt tttgcaggcg agttattaga ataaagaaat gatacgcgcg
     5281 aggtcagaga aaatcttcac aatcaagact ttgaaataac ggagatggat aagaattttc
     5341 tactaaatta atcgcaagag aatattcatt ctctatttgc ggcagatcac aaaaaaaagg
     5401 tgcacatttg tgcacccaag gatgaaagct gacagcaatg tcagccgcag accactttaa
     5461 tggccagtcc tccgcgtgat gtttcgcggt atttatcgtt catatctttg ccggtttcat
     5521 acatcgtctc gatcacttta tcgagtgaaa cacgcggtgc cgaggtgcgg cgcatcgcca
     5581 tccacgcggc gtttactgct ttcacggcat taatggcatt acgttcaatg cacgggattt
     5641 gtacctgtcc ggcaaccgga tcgcaggtca gcccaaggtt atgctccatc gcgatttccg
     5701 ccgcattgca tacctgcgcc ggactgccgc ccagtagttc agttaaccct gccgccgcca
     5761 ttgaacaggc cacgccaatc tccccctgac agccgacctc cgcgccagag atggaggcgt
     5821 tcattttata cagcgcgcca atagcccccg ctgccagaaa atagcgggca attgaccgct
     5881 cgtttaccgg acgacggaac ttatcgtaat aagccagcac tgccggaata atgccgcacg
     5941 caccgttagt cggtgccgtt accacgcgcc cgccagctgc gttttcttca ctaaccgcca
     6001 gcgcgtacat gttgatccag tcgatgacat tcatcggatc gttagagatg ttatcgctag
     6061 aaaccagctg acgacgcagc gctacggcac gacgcggcac attgagcgga ccaggcagca
     6121 cgccttcagt gttcatcccg cgttcaatac cgtcatgcat cacttgccag atacgggcaa
     6181 atcctgcgtc aatttccgct ttgctgcgca gcgccagctc gttgtgcatc atcaggccag
     6241 atatagacag gccgttgtaa tcacacattt tcagcagttc acctgctgag tggaaatcgt
     6301 aaggtacgga cgtttcgaca tcgtgcgaca ggccgaagtg ttcttcttcg acaataaacc
     6361 cgccgccgac agagtaatag gttttactta atagcgcttc ctgtcccttc caggcagtga
     6421 tccgcattcc gttctcatgg cgaggcaaca tttcgggatg gaagataatg ttctttgcta
     6481 caggaaaatc aacaatatgc gcaccagatg ccactggcag ccgcccgctg cgcgttacta
     6541 actctataaa tgcagggatc tcatcaatga caacatcctg cggactgttt cctgccagtc
     6601 ccatgataat ggcgacatcc gtggcatggc ctttgcccgt cagtgacaac gacccataca
     6661 gatcgaccac aatatggctc gtcgcggtta ataagccgct actttccagc cgatcaataa
     6721 aactttttcc ggcattcatt ggccccacgg tatgcgaact ggagggacca atcccaattt
     6781 tgaaaatatc gaatgcacta atcatatcca caccctcgga ttgccgttca gtgaagtgga
     6841 gcggaacgac cttgcgaccg tcccgctcac gaggctttac gcactacgta ctgcgatagc
     6901 ttcaatttcc agcttcacat ctttcggcaa acgcgcaacc tgcacacagc tccgggtcgg
     6961 gtaggtcgcc tgatgctcat cgaagaactg cttatagact tcattgatgg tggcaaaatc
     7021 attcagatcg gtgatgaaca cggtcatctt gatgatatcg cccacgctca gcccggcagc
     7081 aaccacgatc gctttgacgt tttcgaggct taaacgcgcc tgatcttgca catcagccgg
     7141 gatctcaccg gtctgcgggc aaaccggaat ttgcccggag gtgaagacca tgctgcctaa
     7201 atcaacgccc tgaacataag ggccgattgc gcctggggca cgttgcgttt cgataatctt
     7261 tttcatacat cctccggcgt cagagcgcct gggtaaaagt acgtgaaata acatcctgtt
     7321 gctgttcacg ggtcagtgcg ttgaagcgca cggcgtagcc agagacacgg attgtcaggt
     7381 taggatattt ttccgggtgc tcgatggcat ccagcagcat ttcccgattc attacgttga
     7441 cgttgaggtg ttgaccgcct tcgacatccg cttcgtggtg gaaataccca tccagcaggc
     7501 cgacaaggtt ggttttacgt actggatctt ctttgcccag cgccgcagga acgattgaga
     7561 aggtgtacga gatcccatct ttggcgtagg tgaacggcag tttcgccacc gacgtcaatg
     7621 aggccacggc acctttgcgg tcacgaccat gcatcgggtt agcgcccggc gcgaacggtg
     7681 ttccggcgcg acgaccgtcc ggcgtattac cggttttctg gccgtacacc acgttagaag
     7741 tgatagtcag aatcgactgg gtagggacgg cgttgcgata ggttggcagc gctttaattt
     7801 tcttcataaa gcgttcaacc aggtcgcagg caatgctgtc tacgcgctcg tcgttgttgc
     7861 cgtactgcgg atattcaccg tcgatttcaa agtccaccgc caggccgttt tcgtcacgga
     7921 ttggtttcac gcgggcatat ttgatggcag acagggagtc cgtcgccacc gacaggcccg
     7981 cgatgccgca tgccatagtg cgatagacat cacgatcgtg cagcgccatc agcgaagctt
     8041 cgtagctgta cttgtcgtgc atgtagtgaa tgatattcag cgcgctgatg tactgcaccg
     8101 ccagccagtc catgaagtga tcgaggctgt ccatcacttt gtcgtaatcc agcacgtcgt
     8161 ccatcagcgg tgctgttttc ggcccgacct gaatcttcag cttctcgtcc accccgccgt
     8221 taattgcgta cagcagtgtt ttcgccaggt tagcgcgtgc accaaagaac tgcatttgct
     8281 taccaatcac cattgggctg acgcaacaag caattgcgta atcgtcgctg ttgaagtcag
     8341 tacgcatcag atcgtcgttt tcatactgca aggaagaggt gacgatcgac acctgcgcgg
     8401 catatttttt gaaggcaatc ggtaattctt ccgaccaaag aatggtcagg ttaggttccg
     8461 gtgccggccc catagtgtgc agggtgtgca aatagcggaa ggagtttttg gtcaccagcg
     8521 tacgaccgtc cagccccatc ccgccgatca cttccgtcgc ccagattggg tcgccggaga
     8581 acagcgaatc aaattccggc gtacgcaaga agcgcaccat acggatcttc ataatgaagt
     8641 gatcgatcag ttcctgtgcc tgctgctcat tgagtacgcc tgctttaaag tcgcgctcaa
     8701 tgtagatatc gaggaacgac gccgtgcgcc ccagcgacat cgcgccgccg ttctgtgatt
     8761 tcactgccgc cagataagcg aagtagagcc actgcaccgc ttcctgcgca ttctgcgccg
     8821 ggcgagagat atcaaagcca tatttcgccg ccatttcctg aatctgcaac agcgcatgac
     8881 gatgctctgc cagctcctca cgcagacgga tggtggcttc cagatcttcg cctttttcca
     8941 gacgggactg gagatcggca aattgcagtt cgcgttcacg caccagataa ctgatgccat
     9001 acagcgctac gcggcgatag tcaccgataa tgcgcccacg gccatagcca tctggtaaac
     9061 cggtcagcac gccagattta cggcagcgca gcatatccgg tgagtaaaca tcaaatacgc
     9121 cctggttatg ggttttacgc agatcggtaa acagatattc aaattcactg tccatttcac
     9181 ggccataggc gtggaatgaa cttttaatca tattaatgcc accgaacgga tgcagcgcgc
     9241 gctttaatgg cgcatccgtt tgcagaccaa caattttttc cagcggctgg ttaatatatc
     9301 ccgcatcatg agcggtaatt gtggtggcaa tattggtatc gaaatcaacc ggcgcgtggg
     9361 ttgcgttttc gatacggatg ccttccatta ctttttccca caattccgtg gtggcaggcg
     9421 tcgcttcggc gaggaaagat tcatcgcctt catacggtgt atagttatgt tgaataaaat
     9481 cgcggacatt aatttcgttt ttccagtccg tacctttaaa gccaagccat gcgtcggcgt
     9541 acagcttatc gctggtatca atatctatct tcatgaaaaa taatctctct acaatacttc
     9601 aactaaatta tgcaaattct gcgggcgcgt taactttgcc taaatgaatg gcatccaaag
     9661 caatcatttt ttcttcgtta gtcggaataa cggcacaaat gacacgcgca ttttcactgg
     9721 aaacaattcg ctctccaaaa gagttagagc gattattcat ttctgtatca atcactacgc
     9781 ctaatacagc caaatgttcc atgaccagac gacgaattaa acttgaattc tctcctattc
     9841 cgccggtgaa tataattcca tccaggcgac gtaatgaagc tgcgtgtccg gcaatatgac
     9901 gggcaattcg gtgaacaaag gttttaattg ccagttgcgc gcgttcgtga ccttcatgcc
     9961 aggctttttc cagaacacgt aaatccgaag aaagaccgga aatacctaat aatcccgact
    10021 ctttattcac tacgcgttcc aggtcaccca ggctctggtt ggtttggctg gcgacccagg
    10081 acatcgcacc aaagtcgaca tcgccactgc gggttcccat catcaagcct tccagcggcg
    10141 tcattcccat tgaagtatca acactctgac cgttgcgaac cgcgcagatt gacgcgccat
    10201 tgccaagatg cgccacaacc aggccggagt catcttccgc cagattcagc agagaatgtg
    10261 cgcgctggga aacatagcgg tgcgacgtgc cgtggaaacc ataacggcgt acacctaact
    10321 cttcataata tttccacggc aggccgtata aataagcttc cggagccatc gtctggtgga
    10381 aactggtatc aaataccgcc acctgagtta cgcccggaaa taattgctgc gccgattcaa
    10441 taccacttaa attggcgtaa ttatgcaggg gtgccagtgg agaaacgcga cggatattat
    10501 caatgacttc atcggtaata atggcggact cggtaaaaat actgccgccg tgagcgatgc
    10561 ggtggccaat taaggccaca ctgtcattta aattccgttt ttccagttca aatgcaattg
    10621 ccttcaatgc accttcgtag ctgtggtgag ccagcggtgc tggctctccc ccatttacgg
    10681 ataagaatgc attttccgag ttaataccgt cggcaatacc tgacattaat acttcacagt
    10741 cgctggcatc gagcacggaa aacttaatcg aagacgaacc acagttaata accaaaacaa
    10801 ccggaaattc attcatctct tttctcatcc tgagttacgg attaaaacag tttgtatacg
    10861 atgttcagga tggtcagcag accaatcacg gtaacaaaca cgttatccag acgaccacgg
    10921 tatttcgcca gagacggcgc tttacggatg gcatacatcg gcaacaggca cagcagggat
    10981 gcgataatcg gtgcgcccat ggcttcaatc aggtcgagga tgttcgggtt ggcgtaggca
    11041 acaacccagg tggagcccat gatgaagatc atgctgatag tgttcagttt acccagcgac
    11101 actttagttt tgtcgccttt ataaccaaac ttcaggacca ggccattcag accttccagc
    11161 gttcccagat agtgaccgaa gaaagatttg aagatagcca cgagtgcgat gatggaagcc
    11221 gcatattcca gtgtaatcgc gaacgttgtt ttggtaccgg tcatggacgc aaagtggtta
    11281 gccagataag aaagcactgg aatattctgc gctttggctt ccgccatgtt ggccggagac
    11341 agcgtaaata ggcagctaaa ggcaaagaac atcaccactg caaccatcag catgctggca
    11401 cgagaaatga tttgggaaca tttacgttcg gtgaagtcgc gaccgaagtc tttctcatac
    11461 tcttcacgtt tagaaaccac gaaggaagag acgattggcg agaaattaaa ggagaaaacc
    11521 atgatggaaa tccccagcca gacggtgatc aggataccgt catgaccggt taacgacagc
    11581 gaaccgaggt caacctggtc gataactgca gagttccagt aagggatcag cgacagagaa
    11641 atcagcacca ggctggcgat aaacggccat accaggtagc tcatcacttt aaccatcaga
    11701 tccttaccaa accagatgac gaaagccatc agcagcagca ggaacagcgc cacaaagccg
    11761 cgattcagcg gtgcaaagcc gagctggttt tcccagaacg tcataaaggt attggtaata
    11821 gtaacgccat aaatccacag cagtgggcaa atcgcgaaga agtacaggaa cgtgataacc
    11881 acgccgccag ttttaccaaa atgctcttcc accgtttccg taatgttgcc ggaagggtta
    11941 gagccagaaa gacatagacg cgccagcgcc cggtggcaat aaaatgcgat ggggtatgcc
    12001 aataccaaca tcagaagaat cgggatcagt ccgccaaaac ctgcgcggat agggaagaac
    12061 agcaccccgg cgccgattgc cgtaccaaac aagcctaacg tccatgtggt atctgattta
    12121 cgccaggacg attgttttgt ctggctggat acaatgctat ctgaagtact catatcctat
    12181 cctcaacgaa ttaattaagc gtcaacgaaa ccggtgattt gagagacgcg agaaagatcg
    12241 atattgccgc cggaaataat actgacggtt tttctgtttt gaatatattg gtctaattta
    12301 ccgcttaata atgcagcaca tgccagagcg cctgcgcctt cggtgacgac tttattgcgc
    12361 tgaattaagg caatcatact gtttctgatt tcgtcttcgc tgaccagcac gatgtcatcg
    12421 actaattcac gaacgatttc gtaagttaaa ttacccgggc gggagacatc acaaccatcc
    12481 gccagggtgc cggtagttcg gtgcgtggtt atttctccgg agtggaaaga agccgccatg
    12541 ccgtgaacgt tttcagactg tacgccaata acacgaatgg tcgggttaat agatttaatt
    12601 gccaccgcaa taccagcaat taaaccgcca ccaccaattg gcacaatcac gttatcgaca
    12661 tcatagagat cttccataat ttccagacca atcgttccct ggccagcaat cactttcgga
    12721 tcatcgtaag gtgggataaa aatacggcct tccatttcga caatttcgct cactttagcg
    12781 atagtgtcgt tgaagttatc accatgcaga acgacttctg cggagtagtc gcacgttgcc
    12841 gctactttgg attttggcgc accttttggc atcaccactt taccgtcgat acccagcatc
    12901 gcgcaggaga gcgaaacccc ttgcgcatgg ttgcccgcag aacaggccac cacgcctttg
    12961 cgtttttccg catcggtcag tgaacttaat ttattaaatg cgccacgaat tttaaatgaa
    13021 cccgtacgct gcatattttc aaacttcagg aatatttcac ctttgcaacg ttcactaaaa
    13081 tagttggagc gaggcatgcc tgttttataa attcgcccag ccagtcgttg tttcgcttca
    13141 ataatatcat caatagcaat cggcagatcg tatgtaatat gcattataaa acctcttcgc
    13201 ctgaattaaa tatgtaggta accgtaaccg acacctgcaa aacagacagg tggattattt
    13261 atgtttaaat aaaaacaaat aactaaccaa cttctattaa ttgccttcgt ctacacccat
    13321 tataagatga atactctttg gctaattcca ccaaaaccga tgctgctttt ttaatacgat
    13381 aatttttcga ccataccgcg gcatattgtg ccacaggtaa tgtttcttca accggaatag
    13441 taataaattg attagaacca aaaggtgacg tcatatcaca aggaattaca gttaagaaat
    13501 cagcattgag aacaagatta taaattgtca cgactgagtc ggttttaacg atgttttcaa
    13561 tactgatgcc atttctttgt aacgtagtaa gcagttcgct gtagtacccc atattagttt
    13621 gtggcaacac ccactgttcg ttcttcaacg actccagcgt ggtggtgccg gtgcatgttc
    13681 gggacttact ggctaccagc acaaactcgg actcgaacag cggctcaaca tgtaaatcct
    13741 gaagcttcat ttctgcactt aacgtaccaa tcgcaaaatc cagtcgaccg tcgcggattg
    13801 ccggtaagaa cgaagacagt tgcgcttcat acatagaaac ctgcgctttc gggaacacct
    13861 ctttgaactt gttgatcatc cctgacataa aagtaaaacc aatcaatgaa ggaaaaccaa
    13921 atgagacttc caccaccgcc tcagaagaca taccgcttat ctcattaacc atatttttca
    13981 tttcacgggt aatggattcg gaacgggaga gtaacaattg accggcaggt gttaatgtta
    14041 caccggtatt tttccgcacc actaattcca caccaaaata atcttcaata tcgttaatga
    14101 ttttactgac ggccggttga gttaacccta attcttttgc agccgagccg atagagccac
    14161 ttctaatgac ttcctgaaag actaccaggt gctgcgtttt cggaagaaga atagtgctca
    14221 taacgaccta cgttaattac ctcattgacg gcatgaagtg tatcaaaatg aaatgaacag
    14281 gatatgtgcg accactcaca aattaacttt caatactttc cagggtatcg tcat
//
LOCUS       DAZPDU010000110        14297 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZPDU010000110 DAZPDU010000000
VERSION     DAZPDU010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14297
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.111"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            19..1236
                     /gene="rtcB"
                     /locus_tag="ACHURS_002933"
     CDS             19..1236
                     /gene="rtcB"
                     /locus_tag="ACHURS_002933"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFR1238608.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRDLAY
                     FMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEEINC
                     HHNYVQKEQHFGEEIYVTRKGAVFARAGQYGIIPGSMGAKSFIVRGLGNEESFCSCSH
                     GAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSDLVE
                     VIYTLRQVVCVKG"
     gene            1240..2256
                     /gene="rtcA"
                     /locus_tag="ACHURS_002934"
     CDS             1240..2256
                     /gene="rtcA"
                     /locus_tag="ACHURS_002934"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFR1238609.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRLGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAVVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(2299..5004)
                     /gene="malT"
                     /locus_tag="ACHURS_002935"
     CDS             complement(2299..5004)
                     /gene="malT"
                     /locus_tag="ACHURS_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFR1238610.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSWICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKTFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            5615..8008
                     /gene="malP"
                     /locus_tag="ACHURS_002936"
     CDS             5615..8008
                     /gene="malP"
                     /locus_tag="ACHURS_002936"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFR1238611.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLVGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            8018..10102
                     /gene="malQ"
                     /locus_tag="ACHURS_002937"
     CDS             8018..10102
                     /gene="malQ"
                     /locus_tag="ACHURS_002937"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFR1238612.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVKA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVADQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRNDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(10147..11463)
                     /gene="gntT"
                     /locus_tag="ACHURS_002938"
     CDS             complement(10147..11463)
                     /gene="gntT"
                     /locus_tag="ACHURS_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFR1238613.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIDSIKVGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(11824..12399)
                     /gene="nfuA"
                     /locus_tag="ACHURS_002939"
     CDS             complement(11824..12399)
                     /gene="nfuA"
                     /locus_tag="ACHURS_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFR1238614.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            complement(12458..13141)
                     /gene="gntX"
                     /locus_tag="ACHURS_002940"
     CDS             complement(12458..13141)
                     /gene="gntX"
                     /locus_tag="ACHURS_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFR1238615.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSLLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            13179..13949
                     /gene="bioH"
                     /locus_tag="ACHURS_002941"
     CDS             13179..13949
                     /gene="bioH"
                     /locus_tag="ACHURS_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFR1238616.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSQGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDDWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPRSKSYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            complement(13978..>14297)
                     /locus_tag="ACHURS_002942"
     CDS             complement(13978..>14297)
                     /locus_tag="ACHURS_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFR1238617.1"
                     /translation="LMGLVEQMACLLSSGYANDRQIKGLFNYILQTGDAVRFNDFIDG
                     VAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADIMRFTGVSEEELAA
                     ASQ"
BASE COUNT         3440 a         3565 c         3992 g         3300 t
ORIGIN      
        1 cgaaaagaga aaaacaaaat gaattacgaa ttactgacca ctgaaaatgc cccggtaaaa
       61 atgtggacca aaggcgtgcc ggtagaagcc gatgcgcgtc agcaacttat taatacggcg
      121 aagatgccgt ttattttcaa acatattgcg gtaatgcctg atgtacacct gggtaaaggt
      181 tccaccattg gtagcgtgat cccgaccaaa ggggcgatta ttccggcggc ggtgggcgtg
      241 gatattggct gtggaatgaa cgcgctgcgt accgcgttaa cggcggcaga cctgcctgaa
      301 aacctggcgg agctgcgtca agcgattgaa acggccgtac cacacgggcg tactaccggg
      361 cgttgtaaac gtgataaagg cgcatgggaa aacccgcctt ttaacgtcga tgcgaaatgg
      421 gctgagctgg aggccggtta tcagtggtta acgcaaaaat atccgcgttt ccttaacact
      481 aacaactata aacacctggg aacgggtaac cactttattg aaatctgcct tgatgagtcg
      541 gagcaggtgt ggattatgct gcactccggt tcacgcggaa ttggtaacgc catcgggact
      601 tactttatcg atctggcgca aaaagagatg caggaccaac ttgagacgtt gccgtcgcgt
      661 gatctggcgt actttatgga aggtacggaa tactttgatg attacctaag agccgtggcc
      721 tgggcgcagc ttttcgccag ccttaaccgc gatgcgatga tggaaaacgt ggtaacggca
      781 ttgcagagca ttacgcagaa aacggtcaga cagccacaaa cgctggcgat ggaagagatc
      841 aactgtcacc acaactatgt gcaaaaagaa cagcactttg gtgaagagat ctacgtgacg
      901 cgtaaaggcg cggtgtttgc gcgtgctggt caatatggaa ttattccagg gtcgatggga
      961 gcaaaaagct ttatcgtccg tgggctggga aatgaagaat ctttctgttc gtgcagccac
     1021 ggtgccgggc gggtaatgag ccgtactaaa gcgaaaaaac tgttcagcgt ggaagatcaa
     1081 attcgtgcca ccgcgcatgt ggaatgccgt aaagatgccg aagtgatcga cgaaatcccg
     1141 atggcgtata aagatattga tgcggtgatg gcggcacaaa gcgatctggt ggaagttatc
     1201 tataccctgc gtcaggtggt gtgcgtaaaa ggataaatga tgaaaaggat gattgcgctg
     1261 gatggcgcac agggcgaagg tggcgggcag atcctgcgct cggcgctgag cctgtcgatg
     1321 ataaccggcc agccatttac catcaccagc attcgtgccg ggcgggcgaa accggggctg
     1381 ttgcgccagc atctgaccgc ggtaaaagcg gctgcggaaa tttgtagggc aacggtggaa
     1441 ggcgcggagc tggggtcgca gcgtctggtc ttccggcttg gcaccgtgcg cggcggcgag
     1501 taccgctttg ctatcggtag tgccggaagc tgtacgctgg tgctgcaaac ggtgctgccc
     1561 gcgctgtggt ttgccgatgg accttcgcgt gttgaagtga gcggaggcac cgataacccg
     1621 tcggccccgc ctgcggattt tatccgccgg gtgctggagc cgctgctggc gaaaatagga
     1681 attcatcagc aaaccacgct gttacgtcac ggtttttatc ctgccggagg cggcgtggtg
     1741 gcaacggaag tctcgcccgt ggcatcgttt aacaccttgc aacttggcga gcgcgggaac
     1801 attgtgcaga tgcgtggaga agttctatta gctggtgtgc cgcgccatgt tgctgagcgt
     1861 gaaatcgcta cactggcggg gagtttttcc ctgcatgaac agaatattca taacctgccg
     1921 cgcgaccagg ggccgggtaa taccgtctcg ctggaagtcg aaagtgaaaa tatcactgaa
     1981 cgctttttcg tcgtcggtga aaagcgcgtc agtgccgagg tggtagccgc gcagttagtg
     2041 aaagaggtga aacgctacct ggcaagcccg gcggtagtgg gggaatatct cgccgaccag
     2101 ctggtgctac cgatggcgct ggcgggcgcg ggagaattta cggtcgccca tccttcatgc
     2161 catctgctga ccaatatcgc cgtggtggag cgtttcttgc cggtgcggtt tggtttgata
     2221 gaaacagatg gcgtaacgcg ggtgagtatt gaatgatgta aagatgtaaa ccggatccaa
     2281 tgcattatcc ggctaaactc acaccccata tcccatcatc ttcagcagtt tctgcgcgtg
     2341 ttgtaccgcg gcctgacggt gggcgacgcc gagtttttgg tacagattgc ggatatgcgt
     2401 cttgatggtg gttgccgcca cttccagctc gcccgcaatt tgctcattgc tgtaaccgga
     2461 gtagatcagc cccaggacct gccattcacg ttgggtcaac gggctggtgc ggatcagttc
     2521 tggcacttcc ggatgattca gcagacgttc aacgaaattc tcatcgaaat gggcgaattt
     2581 atgccgatga tgttgattga tttctcgcag aatacgctgc gcgcgatgct gttccagttc
     2641 cggcagcgta ttgagttgaa tcagctggcg cagttgttgc gccatcgctt cgccttcgat
     2701 gacaaaatga ctgataaatc cggtgcgatt cgccagtttt aatgcgtcca gcaacacgcg
     2761 ctgggcgtca cttttacgtc cggcctgcca gtacagttga ttaagcagca acaggttacg
     2821 gttgagatcg ctcatcaacc gcagactgcg ggcattttca ttgagttctt cgagaacaat
     2881 ttccgcaggt tcaaactcgc ccagcaagat ttgtgcgcgg gcaatgttgc gccattgacc
     2941 ttgcaggaag tggttgttcg caaactccgg tttagccgta tgacgcaacc agttggcagc
     3001 ggcggcttta tcgccggtca tttgccagta aatcacccgg actttgttgg cgttagagat
     3061 ccagtcgctg tgatatttgc cattccccag caggttttcc agacggttca gctggctacg
     3121 ggcgttatct aaatcaccac gggccagcga gcattgaatc aacattgcca ggcactgaag
     3181 ctgttgctgt ggctgataag acgacaagac ttcaatcccg ctacgcgccg acgcttcggc
     3241 ttcatccagc cgtgcccagg cccataacag ttgtgctcgg atgcgcacca gaaactcatg
     3301 catcggcagc tgttccagat gctgctcgtt gatcagctgg aacgtttttt cctgtgtttc
     3361 ccacgcggtt tgcaggaacc cttgggcaaa cagaatttca ctttgctgga ttaaactcca
     3421 taaggcgtag tgccagacgt cgtgctggcg tgccatctgt tcggtttgct gcattagcgc
     3481 cagtgagcgg gtcaattcgc ctttgcagtg cagcacttca cccagcaccg aggttgctac
     3541 aatgcggcta tagaaccagc ccggtggcag ctcttccagt gccagttttg ccagccgttc
     3601 cgcttcatcc ggattaccat cgttaatcgc cacctgggcg cgcagagcgt taaattctgc
     3661 gtgcatggtg ccttctctga tgtccttgat ttcatgttca gcacgggcta gcagggtgtt
     3721 aacttcgcca tagcgatgtt ggctttgcat tagccacgcc tgcaataaca ctaactgcgg
     3781 attttccagc aaactgtccc acggcagggc cttaagcgac tcttccagca gcgacagttc
     3841 gctatggttg aacaaactcc aggcgtgatt aagcagaata tcgcgcagca tcagcgcatc
     3901 gcctgccgcc agcgcatgat gaatcgcttc gctgggaaat ccctgggcca tccagctttc
     3961 tgcggcggca cggtggattt ccggcagctc cgccgccagt tcccactggc agcgctggcg
     4021 caggaagttg ccaaacagcg ggtgatagca gaaccactcg ccggtatcat ccatccgctg
     4081 taaaaacagc ccctgacgct caatctcttc gagacgcatt tgcccgtttt cttcgccagt
     4141 cacacgggtg atgagggcat cgttcattga gcgcaaaatg gcgcttttca acagaaaatg
     4201 gcgcgttgcg agatcgacgt tatccaaaac ctcatcgacc agataatccg aaagatggct
     4261 ggcattgatt cccgccaggc ggcgtgccga cttatgggct gagtgggtat tctgccgggc
     4321 ggagagggcg attagctgta gtgccgtcgc ccaaccggaa acgtcatcgc aaatccaact
     4381 gctttctgcg gcttcaatcg gcgatgacag acggcaatca aaaaactgct tcgcttcctg
     4441 atgggtaaat gccagttgct gactgccaat ttccagcagt tggtcacgaa cacgcagatt
     4501 ggcaatgccc agttgcggaa ggttgcgtga caacaccacc agggtgagat tttctggttg
     4561 atggcgaata aagaagcgca ttgactcgtg gatcactgga ttagtgatca gatgatagtc
     4621 atcgatgacc agataaagtg ggctatgcca ttccgccagc tcaatgaaaa gctgggcgaa
     4681 gagtgacgtc aggctggcat attgccgttt ttgcgccatc gtctcacata tcgcacagtg
     4741 accgttggtt gcctgctgca cggcggcaat gagatagctg gcgaaacgct cttgctggtt
     4801 atcaccttca tccagcgagt accagccgat atcgtttttg cctgccgccc actgggaaat
     4861 gagggtggtc tttccgtagc ccgcaggact cgtgatcagc gccagccgga agttgttcgc
     4921 gccggaaagt ttagccagca ggcgctcacg aaccacggta tggtcgagtc gaaccggacg
     4981 acttagtttt gacggaatca gcatagttaa tcacttcact gtggaaaatg aggaaatatt
     5041 atttttttgc gcttcgtaat taatggttat aaggtcggcc agaaaccttt ctaatgcaag
     5101 cgatgacgtt tttttatgtg tctgaatttg cactgtgtca caattccaaa tctttattaa
     5161 caactcacct aaaacgacgc tgatccagcg tgaatactgg tttcccttat gttcatcaga
     5221 ttcatttaag caagggtttc ttcttcattc ctgatgaaag tgccatctaa aaagatgatc
     5281 ttaataaatc tattaagaat gagatggagc acattggata ttttacttgt gaaactgttt
     5341 cactccttta cttaatttat agagttacct tccgcttttt gaaaatacgc aacggccatt
     5401 ttttgcactt agatacagat tttctgcgct gtattgcatt gatttgctgc taatcctgtg
     5461 gtttgcacta gctttaagtg gttgagatca catttccttg ctcatccccg caactcctcc
     5521 ctgcctaatc ccccgcagga tgaggaaggt caacatcgag cctggcaaac tagcgataac
     5581 gttgtgttga aaatctaaga aaagtggaac tcctatgtca caacctattt ttaacgataa
     5641 gcaatttcag gaagcgcttt cacgtcagtg gcagcgttat ggcttaaatt ctgcggctga
     5701 aatgactcct cgccagtggt ggctagcagt gagtgaagca ctggccgaaa tgctgcgtgc
     5761 tcagccattc gccaagccgg tggcgaatca gcgacatgtt aactacatct caatggagtt
     5821 tttgattggt cgcctgacgg gcaacaacct gttgaatctc ggctggtatc aggatgtaca
     5881 ggattcgttg aaggcttatg acatcaatct gacggacctg ctggaagaag agatcgaccc
     5941 ggcgctgggt aacggtggtc tgggacgtct ggcggcgtgc ttcctcgact caatggcaac
     6001 tgtcggtcag tctgcgacgg gttacggtct gaactatcaa tatggtttgt tccgccagtc
     6061 ttttgtcgat ggcaaacagg ttgaagcgcc ggatgactgg catcgcagta actacccgtg
     6121 gttccgccac aacgaagcac tggatgtgca ggtagggatt ggcggtaaag tgacgaaaga
     6181 cggacgctgg gagccggagt ttaccattac cggtcaagcg tgggatctcc ccgttgtcgg
     6241 ctatcgtaat ggcgtggcgc agccgctgcg tctgtggcag gcgacgcacg cgcatccgtt
     6301 tgatctgact aaatttaacg acggtgattt cctgcgtgcc gaacagcagg gcatcaatgc
     6361 ggaaaaactg accaaagttc tctatccaaa cgacaaccat actgccggta aaaagctgcg
     6421 cttgatgcag cagtacttcc agtgtgcctg ttcggtagcg gatattttgc gtcgccatca
     6481 tctggtgggg cgtaaactgc acgaactggc ggattacgaa gttattcagc tgaacgatac
     6541 ccacccaact atcgcgattc cagaactgct gcgcgtgctg atcgatgagc accagatgag
     6601 ctgggatgac gcttgggcca ttaccagcaa aactttcgct tacaccaacc ataccctgat
     6661 gccagaagcg ctggaacgct gggatgtgaa actggtgaaa ggcttactgc cgcgccatat
     6721 gcagattatt aacgaaatta atactcgctt taaaacgctg gtggagaaaa cctggccggg
     6781 cgatgaaaaa gtgtgggcca aactggcggt ggtgcacgat aaacaagtgc atatggcgaa
     6841 cctgtgtgtg gttggcggtt tcgcggtgaa cggtgttgcg gcgctgcact cggatctggt
     6901 ggtgaaagat ctgttcccgg aatatcacca gctatggccg aacaaattcc ataacgtcac
     6961 caacggtatt accccacgtc gctggatcaa acagtgcaac cctgcactgg cggctctgtt
     7021 ggataaatca ctgcaaaaag agtgggctaa cgatctcgat cagctgatca atctggaaaa
     7081 attcgctgat gatgcgaaat tccgtcagca atatcgcgag atcaagcagg cgaataaagt
     7141 ccgtctggcg gagtttgtga aagttcgtac cggtattgag atcaatccac aggcgatttt
     7201 cgatattcag atcaaacgtc tgcatgagta caaacgccag cacctgaatc tgctgcatat
     7261 tctggcgttg tacaaagaaa ttcgtgaaaa cccgcaggct gatcgcgtac cgcgcgtctt
     7321 cctcttcggc gcgaaagcgg caccgggcta ctacctggcg aagaatatta tctttgcgat
     7381 caacaaagtg gctgacgtga tcaacaacga tccactggtt ggcgataagt tgaaggtggt
     7441 gttcctgccg gattattgcg tttcggcggc ggaaaaactg atcccggcgg cggatatctc
     7501 cgaacaaatt tcgactgcag gtaaagaagc ttccggtacc ggcaatatga aactggcgct
     7561 caatggtgcg cttactgtcg gtacgctgga tggtgcgaac gttgaaatcg ccgagaaagt
     7621 cggtgaagaa aatatcttta tttttggtca taccgtggaa caagtgaagg caattctggc
     7681 caaaggctac gacccggtga aatggcggaa gaaagataag gtgctggacg cagtattgaa
     7741 agagctggaa agcggtaaat acagcgacgg cgataagcat gccttcgacc agatgctgca
     7801 cagtatcggc aaacaaggcg gcgatccgta tctggtgatg gcggatttcg cagcctatgt
     7861 tgaggcacaa aagcaggtgg atgtgctgta ccgcgaccag gaggcctgga ctcgcgcggc
     7921 gatcctcaat accgcccgct gcggtatgtt tagctcggat cgctctattc gcgattatca
     7981 ggctcgtatc tggcaggcaa aacgctaagg aagctcgatg gaaagcaaac gtctggataa
     8041 tgccgcgctg gcggcgggga ttagccccaa ttacatcaat gcccacggta aaccgcagtc
     8101 gattagcgcc gaaaccaaac ggcgtttgct tgacgcgatg catcaacgta ccgccacgaa
     8161 agtggcggta acgccagtcc cgaatgtcat ggtttatacc agcggcaaaa aaatgccgat
     8221 ggtggtggag ggcagcggcg aatatagctg gctgctgacc accgaagaag gaacacagta
     8281 caaaggccat gtaacggggg gcaaagcgtt caatctaccg acgaagctgc cggaaggtta
     8341 tcacacgctg acactcaccc aggacgacca gcgcgcgcat tgccgggtga ttgtcgcccc
     8401 gaaacgctgt tacgaaccgc aggcgttgct gaataaacaa aagctgtggg gtgcctgcgt
     8461 tcagctttat acgctgcgat cggaaaaaaa ctggggtatt ggggattttg gcgatctcaa
     8521 agcgatgctg gtggatgtgg caaaacgtgg cgggtcgttc atcggcctga acccgattca
     8581 tgcgctctat ccggcaaatc cggagagtgc cagcccatac agcccgtctt ctcgccgttg
     8641 gctgaatgtg atttatatcg acgttaaagc cgtcgaagat ttccatctta gcgaagaggc
     8701 tcaggcctgg tggcagttgc cgaccacgca acagacgctg caacaagcgc gggatgccga
     8761 ctgggtcgat tactccacgg ttaccgccct aaaaatgaca gcattacgaa tggcgtggaa
     8821 aggtttcgcg caacgtgatg atgagcagat ggccgcgttt cgccagtttg ttgcagatca
     8881 aggcgacagc ctgttctggc aggcagcctt tgatgcgcta catgcccagc aagtgaaaga
     8941 ggacgaaatg cgctggggct ggcctgcatg gccagagatg tatcagaacg tggattcacc
     9001 agaagtgcgt cagttctgcg aagaacatcg taatgacgtc gatttttatc tctggttgca
     9061 gtggctggct tacagccagt ttgccgcctg ctgggagata agccagggct atgaaatgcc
     9121 gattggcttg tatcgtgatc tggcggttgg cgtagcggaa ggtggggcgg aaacctggtg
     9181 tgaccgtgaa ctatattgcc tgaaagcgtc ggttggcgcg ccgccggata tcctcggccc
     9241 gttggggcag aactggggat taccgccaat ggacccgcat atcatcaccg cgcgtgccta
     9301 tgaaccgttt atcgagctgt tgcgtgccaa tatgcaaaac tgcggcgcat tacgaattga
     9361 ccatgtgatg tcgatgctgc gtttgtggtg gataccgtat ggcgagacgg cagatcaggg
     9421 cgcgtatgtt cactatccgg tggatgatct gctctcgatt ctggcactcg aaagtaaacg
     9481 tcatcgctgt atggtgattg gtgaagatct cggtaccgta ccggtagaga ttgtcggtaa
     9541 gctgcgcagc agcggtgtgt actcctacaa agtgctctat ttcgaaaacg accacgagaa
     9601 gacgttccgt gcaccgaaag cgtatccgga gcagtcgatg gcggttgcgg cgacacatga
     9661 cctgcctacg ctacgcggtt actgggagag cggggatcta acgctgggca aaaccctggg
     9721 gctgtatccg gatgaagtgg tactgcgcgg tctgtatcag gatcgcgaac tggcgaagca
     9781 agggctgctg gatgcactgc ataaatatgg ttgtctgcca aaacgtgccg ggcataaggc
     9841 atcgttgatg tcgatgacgc cgacgctgaa ccgtggtttg cagcgctaca ttgccgacag
     9901 taacagtgct ctgttaggac tacagccgga agactggctg gatatggccg aaccggtgaa
     9961 tattcctggc accagttacc agtataaaaa ctggcgacgc aagctttccg caacgcttga
    10021 gtcgatgttt gccgatgatg gcgtgaacaa gttgctgaag gatttggaca gacggcgcag
    10081 agctgcagcg aagaagaagt agagtgcggt tgatgccgga tgcgatgcat ccggcagtca
    10141 gtgttatcaa atcaccatat tcagcagcag acagcctacc agaccgcaca ccgagataat
    10201 ggtttccagc atcgaccagg atttgatggt ctcgccgata gtcaggttaa agtactcttt
    10261 gaacagccag aagcccggat cgttcacatg agagaaaatc acactaccgg aacccaccgc
    10321 aataaccatc agctctgggc taacacccgt cgttgcaatc agcggtgccg cgataccacc
    10381 cgcagtgatt gccgcaacgg ttgcggaacc cagcgcgata cgcagtacgg cagcaatcga
    10441 ccaggccatc agcagcggag aaatgttggt ttcgtgcatc atggaagcaa tgtatttgtc
    10501 cacgccgctg tctaccagca cctgcttgaa cgcaccgcca ccaccgatga tcaacagcat
    10561 catcgcaatg attttgatgg aagaaaccag cgtgtcgtta atctgatcca ttgaacgacc
    10621 acggttcaga ccaaaggtga acatcgcaat cagcacggca atcagcgttg ccattaccgg
    10681 gtcaccgagg aactccgcta ccggcaggaa agcgtgacct ttcggcagga tcatttcggc
    10741 aatcgcacgc atcgccatca gcacaaccgg caccagagaa gtccagacgc tgacgccaaa
    10801 gctcggcatc tcttcttcgc tgaaggtttt cgcgctgtag agaccttccg ggattggctt
    10861 atcgatacct ttcaacacgc gagcgtaaac cggaccggcg agaatcacgg tcgggattgc
    10921 cagaatagta ccgtacagca gggttttacc catatcggca ttgaaaatgg tggcaatcgc
    10981 agtcgggccc ggatgcggtg gcaggaagcc gtgggtcaca gacagtgcag ccgccattgg
    11041 tacaccaaca tacagcagtg gaatattcgc ggaagccgcg atggtaaaca ccagcggcag
    11101 catcagcacg aagcccactt catagaacag ggcaaaacca acggtaaaac cggttaacac
    11161 caccgcccac tggatgtgct ttttaccaaa tttggcaatc agcgtggtgg cgatacgttg
    11221 tgcgccaccg cagtctgcca gcattttgcc cagcattgcg ccaaaaccca tgatcagggc
    11281 aaggctaccg agcgtcccgc cgacaccgac tttgatggag tcaataactt tatccagcgg
    11341 cattccttgc attaatccaa cagcaagcgc cacgaggacg agagcgatga agccgttcat
    11401 tttgaagcgg atcatcagga gcaacaacaa gattacaccg atagcaacaa tgactaatgg
    11461 catgatttac ctggcctttc atttgttatg ggtaacgtca attttctgac gacaaactct
    11521 aattatccca atcgggaaca gagatattgc ggcaccacga ctgataccca ctaaaactaa
    11581 ttattgtagt cagatgtcag gagtatgttt ggtacccatg tgaatgatac gggtaacatt
    11641 tggcgtttga gaatcaccag agcggggtaa atttaaatta tgagaggttg gtcatattat
    11701 cgcggggaaa cgaagggagg gatttgccaa agcaatgctg tgccaacgtc tggcacatgt
    11761 tcaacgtagg cccgaaatga cgctttagcg tcgcatcggg caatctacaa aagaggggat
    11821 aacttagtag taggagtgtt cgccgcgctg gtgttcggtg agatcgcgca cacctttcag
    11881 ctccgggaat tcgttcagca gctgcttctc gatcccttct ttcagcgtca catcgaccat
    11941 ggaacaaccg ttacagccgc cgccaaattg cagaatggcg taaccgtctt cggtgatttc
    12001 catcagcgaa acgcgaccac cgtgaccagc aagctgtggg ttgatctgcg actgcagcat
    12061 atactccacg cgctccatca gcggtgcatc gtctgccact ttacgcattt tggcgttcgg
    12121 ggctttcagc gttaactgag aacccaactg gtcggtaaca aagtcgatct ctgcatcttc
    12181 caggtatggt gcgcttaact catcaacata cgcggtcagc aggtcaaatt tcagggcagt
    12241 gtcggtggct tccacggcat ccggcggaca ataagaaaca ccacattcag cgttaggcgt
    12301 gccagggtta atcacaaata cgcggatttg tgtcccttct tcctgatttg ccagcagttt
    12361 ggcaaagtgc gcttgtgcag catcggaaat acggatcata gtaatggcct aatagttgac
    12421 tattttagtt ggttataata cgcccatcat cgaggctcta caaggttcga caaaggcacc
    12481 agacctggac agtcgccgca ccattgcgta aaagcaactg cgcaatctct gcgacggtac
    12541 ttccggtggt aacgacatca tccacaatca ccatatggcg accttgcacg ggcaattcaa
    12601 gacgaaaggc atttttcagg ttgcgcttgc gcagccgggc actgagaaaa tgctgggtcg
    12661 cagtggcccg tgtacgtgtg acggcttcgc tatcccattg gcagtgcaac cagcgtgata
    12721 acggctgaca cagcaaatcg ctctgattaa atccccgacg ccagtgacgc cgctgccata
    12781 acggaacgct gatgatgcga tccggcaatt gcaacccggt ggtgcgacga gcgtgtaaga
    12841 cttccaatag taacagacgt gacagggcgc tggcgatttc actgcgccgg gaaaatttaa
    12901 gctggtggat gagcagactt aacggcggcg catagtcggc aaccgtgacc agtctttgcc
    12961 agggcggcgg tttttgcagg cagcgaccgc agggaagatg ggagtgtgtg gcgggtaatc
    13021 cacattgtgg gcataacgtt ttatctgtgc gggtggcgcg tgaacagacc gaacaaatcc
    13081 cccaatgacc taacgccagt ggcattcggc atagccagca taatcccggt actgttagca
    13141 tatgttcatc cttgtaagtc aaaagagaac aatagcggat gaataacatt tggtggcaga
    13201 ccaaaggtca ggggaatgtt catcttgtgc tgctgcacgg atggggactg aatgccgaag
    13261 tgtggcgttg cattgacgag gaacttagct cgcattttac gctgcacctt gttgatctgc
    13321 ccggcttcgg gcgcagccag ggatttggtg cgctgtcact tgctgatatg gccgaagccg
    13381 tgctgcaaca ggcacctgat aaagccattt ggttaggctg gagtctgggc gggctggtgg
    13441 caagccagat tgcgttaacc catcccgagc gtgttcaggc gctggtcacc gtggcgtcgt
    13501 caccttgttt tagtgctcgt gacgattggc cggggataaa accggacgtg ctggcgggat
    13561 ttcagcagca actcagtgat gattttcagc gtacagtgga gcggttcctg gcgttacaaa
    13621 ccatggggac tgaaacggcg cgccaggatg cgcgggcgtt gaagaaaacc gttctggcgt
    13681 taccgatgcc ggaggttgac gtgcttaatg gcgggctgga aatcctgaaa acggttgatc
    13741 tccgtcagcc gctgcaaaac gtgcccatgc cgtttttgcg attgtatggc tatctcgatg
    13801 gtctggtgcc gcgcaaagtg gtgccgatgc tggataaact ctggcctcgc agcaaatcat
    13861 atatcttcgc caaagcggcc catgcgccgt ttatttcgca tccggtcgag ttttgtcacc
    13921 tgctggtggc gttgaagcag agggtgtagg caacttttga aatggcgaga cagaaattta
    13981 ctggctcgcc gcagccaact cttcttctga caccccggta aagcgcatga tgtccgcaag
    14041 agggactccg gattcaagca ttattttggc tatatgcagg gctttggatt gttccccctc
    14101 ctgccgcaat ctttccgcaa ttgtcattaa actctccttg tgtttcggtg aacgttcggc
    14161 aacgccgtcg ataaaatcgt taaaacgtac cgcgtcgccg gtttgcagta tgtaattaaa
    14221 cagccctttg atttgtctgt cattagcgta tccactactt aataagcagg ccatttgctc
    14281 taccagcccc atcaggt
//
LOCUS       DAZPDU010000111        14217 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZPDU010000111 DAZPDU010000000
VERSION     DAZPDU010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14217
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.112"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..183)
                     /locus_tag="ACHURS_002943"
     CDS             complement(<1..183)
                     /locus_tag="ACHURS_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1238618.1"
                     /translation="MTAFRAAFKAYRMHQVLILPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSA"
     gene            complement(225..762)
                     /locus_tag="ACHURS_002944"
                     /pseudo
     CDS             complement(225..762)
                     /locus_tag="ACHURS_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(1123..1224)
                     /locus_tag="ACHURS_002945"
                     /pseudo
     CDS             complement(1123..1224)
                     /locus_tag="ACHURS_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(1409..1618)
                     /locus_tag="ACHURS_002946"
                     /pseudo
     CDS             complement(1409..1618)
                     /locus_tag="ACHURS_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
     gene            complement(1678..3126)
                     /gene="uxaB"
                     /locus_tag="ACHURS_002947"
     CDS             complement(1678..3126)
                     /gene="uxaB"
                     /locus_tag="ACHURS_002947"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFR1238619.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAISYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFAL
                     AALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDHVIGTQELVAIVLAEKDHWEQ
                     DLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(3353..4303)
                     /locus_tag="ACHURS_002948"
     CDS             complement(3353..4303)
                     /locus_tag="ACHURS_002948"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFR1238620.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGGFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPLTVMLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGL
                     VARMGGEEFAVAVPSVNPVDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRAS
                     YRTLTDDFNKLMVEADTCLYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(4442..4801)
                     /locus_tag="ACHURS_002949"
     CDS             complement(4442..4801)
                     /locus_tag="ACHURS_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFR1238621.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(4801..5727)
                     /gene="glsB"
                     /locus_tag="ACHURS_002950"
     CDS             complement(4801..5727)
                     /gene="glsB"
                     /locus_tag="ACHURS_002950"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFR1238622.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(5791..7179)
                     /gene="sad"
                     /locus_tag="ACHURS_002951"
     CDS             complement(5791..7179)
                     /gene="sad"
                     /locus_tag="ACHURS_002951"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFR1238623.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALELAAAGFRD
                     WRETNIDYRAEKMRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENTLGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            7280..8161
                     /locus_tag="ACHURS_002952"
     CDS             7280..8161
                     /locus_tag="ACHURS_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR1238624.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRTNCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVSDSAKQSYQ"
     gene            8238..9353
                     /gene="yneK"
                     /locus_tag="ACHURS_002953"
     CDS             8238..9353
                     /gene="yneK"
                     /locus_tag="ACHURS_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFR1238625.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNINSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFLNDFIEQF
                     PMFNPGVPIKIAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVLPEVCICFLFLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLELFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATSKTPDLPERH"
     gene            9503..10693
                     /gene="ydeA"
                     /locus_tag="ACHURS_002954"
     CDS             9503..10693
                     /gene="ydeA"
                     /locus_tag="ACHURS_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFR1238626.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(10718..11383)
                     /gene="marC"
                     /locus_tag="ACHURS_002955"
     CDS             complement(10718..11383)
                     /gene="marC"
                     /locus_tag="ACHURS_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFR1238627.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            11595..12029
                     /gene="marR"
                     /locus_tag="ACHURS_002956"
     CDS             11595..12029
                     /gene="marR"
                     /locus_tag="ACHURS_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFR1238628.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            12049..12432
                     /gene="marA"
                     /locus_tag="ACHURS_002957"
     CDS             12049..12432
                     /gene="marA"
                     /locus_tag="ACHURS_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFR1238629.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            12464..12682
                     /gene="marB"
                     /locus_tag="ACHURS_002958"
     CDS             12464..12682
                     /gene="marB"
                     /locus_tag="ACHURS_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFR1238630.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(12739..14178)
                     /locus_tag="ACHURS_002959"
     CDS             complement(12739..14178)
                     /locus_tag="ACHURS_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFR1238631.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     SRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
BASE COUNT         3748 a         3593 c         3204 g         3672 t
ORIGIN      
        1 ggcggataaa tcgacagatt cagcaagatc attgcttaaa aaccgtggat taaaataata
       61 ctctgcgtca acgggaaata ccgcagttgc taagccaagg gcaatggtaa gccgtgcaaa
      121 gcgaggcagt attaacacct gatgcatacg atatgcctta aatgcggcac gaaaggctgt
      181 cattattaat tccgattgag tttattatta attaggtcac tggcttactg catggttgcc
      241 ttaatacgcg gagttaatgc accgtaatca ttgatcgtct gaaaactgat atccccggtt
      301 gcagcatgcg ggatatccac cgatacttca ccttttggcg gcaccatggt gtttgggaga
      361 ttgctatttc ctgctttcat atttgttacg gtaatgaaat agggcgttgg attaataaga
      421 gtgagtttgc taccgctacg acgaaaccga agcattgcag gcgcttcttc aggtgccatt
      481 gccaggttcg tggggcgata aaacattttt atgcggctaa taattgccag ctgtaatgta
      541 ttctcattct tctggtcttt ttccatagca ggaatagctt taacattcac ccagaacagg
      601 ctttcacggt cgcctggtaa ctgatgatta gtagcgttga ttatccgcag cgtattccag
      661 tgcgacagct gcgttggcaa cggtgctatg gcaagcaaag agcaataaaa gtaacactgt
      721 cgccataaag gaaatagagt agggggtacg cgtagtttgc atctgatgca ttctccatgt
      781 gttaagagga taagcgggaa aaccgttaca tcagacaatc gattaatgtt gctaatacat
      841 tggcgagtcg ccgggtttcc agacttaatg cgtttgtaat aaatgttggt tctcttttaa
      901 taggaaaacc ttgctacaaa gtaaatattt taaataacag taagtttaac tactgttgtt
      961 tattgatagt ttagatatta tgtgattttt catagcgctc caaatacaaa ccactcctca
     1021 ttaataatga gattcacatt ggtgaattgc tcgggtgtgc gtagccatgc cacacacacc
     1081 ctgtttcaat ttcagatgac gtatagccac cttaagattg aattatagat attgaacttt
     1141 gaaggtcgca tcagcgtttg cagtaccagc tacggactgc ccgagagcaa tataacgagc
     1201 ctggaatggc agtatattcg tgccacgata acatctttaa atcacaagga cacctaatct
     1261 gaacgaataa cgactaccag aaaagcactt cgcatcactc agacatgatt taacataata
     1321 tacattatgc gcacaaacat aaaccaaggg agaatccagt cgttgggccg gtcatgattg
     1381 ctcattaaca acgaccaaac cccatatctc acttactacc gtattctcgg cttaaccgtc
     1441 catgcaacct caacacattg gtttcaactg ccgtcaccac gttctccgga aaatctgtcg
     1501 gtaatgaagt cttcacgtta tccagtgctg ccggaatcat tctggcaaag tcacttagga
     1561 tttcatgcat ctgcacttcc gggaatctca gcacctttgc tgtcgccaaa aaatgttgta
     1621 actaatatag tgaaataaga aacccggtca aaaccgggtt cagaagtaac ggtgttatta
     1681 gcacaacgga cgtacagctt cgcgcatccc tttttcaaga atcgcatcca ggtcattagc
     1741 aacctgctcg accagaccag gcacttgcgt caggtcctgc tcccagtggt ctttttctgc
     1801 caacacaatc gctaccagtt cctgggtacc gataacatgg tcacgatgct ggctccagag
     1861 ttgctgataa cgttccagcc agtgtgcatc gtcctgtacc ggatatgttt ctccgttacg
     1921 ctcaccacga tagaacgcaa tcaatgccgc taatgcgaaa gtaaggcgcg ccggaagtgt
     1981 gccgtttgcc ttctgccctg ccagcagctg cggcaggatg cgggtgcgga acttggtcat
     2041 accgttgagc gcgatagaca gcagctgatg cttatacggg ttacggaaac gcccggtgac
     2101 tgcactggcg aaagattcca gttcatcacg aggcaaatcc agtaccggaa taatttcttc
     2161 gtagatggct ttttcaacga atgcgcaaat ttcagcatcg ttcatcgctt cacctacggt
     2221 atccagccct gcctggaacg ccaccggcac cagcgcggtg tgcgcaccgt tgaggattgc
     2281 cactttgcgt tctttatacg gtttaatatc gtcaacaatc agcacgttga gcggatattt
     2341 gtccaggcgc agctcagtcg ctaaggattt cggcccctga atcacaaaca ggtaaaagtg
     2401 ttcagcggtg tcgaggaaac catcgtgata acccagttct tcttccagtt tagccacttc
     2461 atcgcgcgga taaccggtaa cgatacggtc caccagcgta gaacagaagc tgttagcctg
     2521 atccagccat tgaataaatg cttctggcag tgcccactct tgcgcatagc gcagcaccag
     2581 ttcacgcaac gcgtcgccat tgtagtcgat caactcacac gggatgatga tccaaccttt
     2641 atccagcgca ccgttgaaat ggctaaagcg ttcgaacagc agacgggtca gttttgccgg
     2701 atagcttatc gctggcgcgt catcgaattt atcgcccgca tggtagctga tccccgcttc
     2761 ggtggtgtta gagaaaacaa agcgcatttc cgggttgtgc gccagtttca ggaattcatc
     2821 gtattcactg tagacgctga tttcacgatt aaccgagcga atcagacgcg cgtcgctgac
     2881 cgcttccccc ttctcattca ggccacggat aatggtggta tacagaccat cctgcgtgct
     2941 cagtgacggc gggaatgaag tttcaatcgg acgaacaacg accacgccag aattcagatc
     3001 ggtgtgctca ttcaggagat cgatttgcca gtcaacaaag gcgcgcagga agttaccttc
     3061 accaaactga atgatacgtt ctggatactg tgcaccggga aaatcgcgac gatttagtgt
     3121 tttcacaatg ggttcccttc tgattagtca tacaacctgt ttgaattggt acgacaggtt
     3181 agcaaacttt aatacgccga acccctgttt tgatcaactc ctgatgatta atgagcagtt
     3241 ttatgagaaa agtgtggcgc ggatcatggt ttaatcgagg aaatacgcct tttcctggat
     3301 cataaagtgg tagaacacat tgcattcaaa tcgcgcggaa tgaataaaga tgtcagacaa
     3361 cttcctcacc gtaacgcata gtgctggtac ggttgcgccc atctttcttc gagcgataca
     3421 gacacgtatc ggcttcgacc atcaatttat taaaatcatc ggtcagcgtt cggtacgatg
     3481 cgcgaccact accgacgcca atacttaccg tgagataaag cgttttttgt tgccaggtga
     3541 atggttgcag ttcaacgcct ttacggattt tttccgccat tagcagacca tcgacaggat
     3601 tcaccgacgg cactgcaaca gcaaattctt cgccgcccat gcgcgccacc agccccttat
     3661 caccgacaat cttctgaata tgccgggcaa acacgcttaa cactttatcg ccacattcat
     3721 gtccatagtt atcgttaatg cttttgaaat agtcgatatc aagcagcatg accgtcagag
     3781 gcacgatcaa cacgcccgtt gagaattgct cactgaacca gtccgcaagc aaaggccaga
     3841 aatccagact atcaatactg accgaaccaa tcgccccgac aatagcgcat aataatgcgg
     3901 caatcagaca gtaattaaat agccgtaagg cacttactgg ctcatactta tttttcccca
     3961 gacgcttatc ccgcacgacc agtaaggcga cggtaacaat aaacatcata ttggagaaat
     4021 tgataaccag tgaaaccagt ccccattcgg tggttatggc atcataaacc agcatcgcca
     4081 cataactgat cgcatagtaa tgcagtcgat taagccagac atagcgggca aacccccccg
     4141 ccattacgcc attcagcggc cagaaaagag aaagttcgtg aaccagccgt aactctgcac
     4201 caataaaata gaacagcgtg gttagcgcaa aaatagcgat gctattacgt agcagatgac
     4261 cttcctggaa caaacggaaa gttgaaatgg ggtgtacatg cattaattat ctgtccatac
     4321 aattgcgggt ggcaattatc tcaacgtaat aagtaaattc tcataaagga tagcatgttc
     4381 gctaacagcg gcggcaaata aaggtaccct gggaatgcta ttttatcgtt ttccgcaatt
     4441 ctcatggctg attcatctgt acaaccagcc agtgatagat taccgccacg atataacgtt
     4501 gttgttcttc gcttaacgaa acaccctgcg gaatgttatg ccacttagcc agacaaccgc
     4561 gacagcaggt cgcggtggcg tgctgagcga taaacaccgg atgcccgcgc atgggggttt
     4621 gcttaccgtc attagccggt aatgccggag caagacgttt agcgacaaaa tccgccgcat
     4681 gttgttcgat gactggcgcg cctttctcca ggcaatactg acgctctttc atgcccagac
     4741 gaaagcgaga gcgaaatttt gaacgggata aacgcgcgaa gagtggatcg agagactgca
     4801 ttaataaacc gaacgcccta actgtttcgt caattgttca aggacggcga tacccgcaag
     4861 cgagttacct gcatcatcca gttccggact ccagactgcg atggccattt catgcggaac
     4921 aatcgccaca ataccgccac caacgccaga tttcgccggt agtcccaccc gccaggcaaa
     4981 ctcccccgcg ttctggtaca taccactggt cgccatcagc gcgttaattt gccgcgcctg
     5041 cattggcgtc actacaggtt catcaatatg aatagctttc ccctgattag ccagaaagac
     5101 aaacgtccga gccagctcta cacagctcat tttcagagcg cagtaatgaa agtagttttg
     5161 cagaacggtt gtcacgtcat gatggaaatt gccaaacgac ttcatcagcc aggcgatagc
     5221 cgcatttcgc gcggaatgtt caaattcgga acgcgctacc accgtatcgt atgaaatatc
     5281 agacacaccg cttaagccgc gcacgacttc cagcatacgt tgccgtggtg cgcttaatcg
     5341 cccttgcaac atatcgcaga ccaccagcgc accagcatta ataaacggat tacgcggaat
     5401 accctgttcc atttccagtt gcactaaaga attgaacggt gatccagacg gatctttgcc
     5461 gacgcgttgc cagatttcct cttcggagta atgacgcatg gcgacaacga gactcagcac
     5521 tttggaaata gactgaatgg aaaaacgttc ttgcgcgtct ccggcctgaa aaagctgtcc
     5581 gtcaacggta cagatagcaa tgcccaatcg ggaaccgtct actgtagcca gcgccggaat
     5641 ataatccgcg actttaccct gaccaatgag cggccgcact tgccgcaaga tgttttctaa
     5701 aattgcatta tccatggcga ctgccacttt ctactcctgg accgcaggtc tgaaaagacc
     5761 tgcgagtata tcagagctga atatgtcgcg tcagatccgg tctttccaca ccgtctggat
     5821 attacagaat tcgtgtaagc cgaaatggga aagctcacga ccaaagccac tctttttcac
     5881 gccaccaaag gccactcgcg cgtcgctggc acaataacca ttgataaaca ccccaccgca
     5941 ttccagacgt gccgccatct gtctggcctg tgtttcgtcg gtggtaaaaa tggtcgctga
     6001 aaggccgaac tcactatcat tagccagttc cagcgcatgt tctgcatctt tcgcaacggt
     6061 gattgccgca acggggccaa acatttcttc ccgaaacgcg gtcatttctg gggtaacatt
     6121 cgccagaacc gttggcggat agtagttacc tgccccagcc atcttttccc cgcccagtaa
     6181 caaacgcgca ccctgcgcca gggttttctc tacctgatga tgcagctcat cacgtaaatc
     6241 aaaacgagcc attggtccga gagtgttctc ttcgtcacgg ggatcgccca ttttcaaggc
     6301 tgccgcagct gccacaaaac gttcggtaaa tgcagaagca attccctctt cgataataaa
     6361 gcgttttgcc gctgcacata cctgtccggt attctgataa cgtccggcta ccgccgcttt
     6421 aaccgccagt tccagatcgg catcgttaag cacaataaac ggatccgaac cgcccagttc
     6481 cagtacgcat tttttcagtg ccgctccagc ctgtgcgcca atagccgctc cagcacgaac
     6541 acttccggtc accgtgacag cagcaatgcg cgagtcttta atcatctgac tgacaccgtc
     6601 gttgtcggca ttcagccagc catatacgcc ttgcgggatt cccgcatctt taaacacctg
     6661 ggcaatgagc tgagcacatc ccatcacatt cggcgcatgt ttaagtaagt agccgttacc
     6721 tgcaagaatg atgggaacag cgccacgcat cacctgccat aacggaaaat tccacggcat
     6781 aatcgccaga atcgtcccca acggtcgata ctcaataacc gcctgctgat tttccaccag
     6841 tgtaggttcc gccttcagca ttgccggacc atgttctgca taccagtcac acaaattcgc
     6901 cgatttcgcc acttcagcgc gcgcctggtt gattggtttg cccatttcgc gggtgatcat
     6961 ttgcgccatt tcttcgctac gggcgcgcag agccttaccg atatcacgca ttttttcagc
     7021 acgataatct atatttgtct cgcgccagtc gcgaaagcct gctgccgcca gctcaagtgc
     7081 gttttcgata tcgttagcgc cagcccacgg cagcacagaa agttgttcac ccgtggcagg
     7141 atttattgaa attgcatgag ttgccggagt aatggtcatc ggggtatctc ctttatgagt
     7201 catggtatga agatacgcag atttactctt gctttaaaat gaataatatt aagccactta
     7261 ttcacgaatc gagaatgtta tggatctgac ccaactggag atgttcaacg cggttgccga
     7321 agctggcagc attacccagg ctgcagcaaa agtgcatcgc gtaccgtcga atctcactac
     7381 ccgtctacgc cagctggaaa cagaactggg ggtagatctg tttattcgcg agaatcagcg
     7441 tttacgtctc tctcctgccg ggcataactt tttacgctat agccaacaaa ttctcgcgtt
     7501 agtggatgaa gcgcggagcg ttgtcgctgg cgatgagccg caaggtttat tttctcttgg
     7561 ttcgctggaa agcaccgcag cagtgcgcat tccggccacg ctggcggaat tcaaccatcg
     7621 ttatcccaaa attcagtttt cactttccac cggcccttcc ggcactatgc tggaaggagt
     7681 gctggaggga aaactgaatg cggcatttat tgatgggccc attaaccata ctgccattga
     7741 cgggataccg gtataccgcg aggagctgat gatcgtcacg ccacaaggtc atgcgccagt
     7801 aatccgcgcc agtcaggtta acggcagtaa catttatgcc ttccgcacca attgttcgta
     7861 tcgtcgccac ttcgagagct ggtttcatgc tgacggtgcc gctccgggaa ctattcacga
     7921 gatggagtcg tatcacggga tgttggcctg tgtggtcgca ggagcaggca ttgcgcttat
     7981 tccacgctct atgctggaaa gtatgccggg gcatcaccag gttgaagcgt ggccgttagc
     8041 tgagcaatgg cgttggttaa caacctggct ggtctggcgt cgcggtgcga aaacgcgtcc
     8101 gcttgaggca tttattcaac tactggatgt gtctgactcg gcaaaacaga gttaccaatg
     8161 aactattttg atagttcttg cggctaatat gctctatata gtgatgttcc gatgacttat
     8221 gactatatgg ggcaaatatg gttacgccag taagcatcag caattacata tctcttcctg
     8281 atgattttcc cgttcgtaat attgcaccac aggtaaaaga agttttaaag gactttattg
     8341 atgcgctaag tacaataata tgtgatgaag agtggcgcac gtctttaaac atcaactcag
     8401 ccacaaaaaa gatatttaat aaccttgata acttatcata tattcagagg acttctttcc
     8461 ggggtaacga cacgctatac aatgaaaagg ttcagtttaa actcacttat cccgtcaaga
     8521 atggaagaca caaagaaaat attgaatttc aggtagtaat aaatttaagc cctatttatt
     8581 tagataattt tcgccatgat ggagaaatta atattttttg cgctcccaac ccaaagcctg
     8641 tcactatggg gcgcgtattt cagaccggcg tcgagcgtgt gctctttctt tttctgaatg
     8701 attttattga acaatttcca atgttcaacc ctggcgttcc gatcaaaata gcgcatacac
     8761 cacatattga acccctgcct ccggatcacc ataccgctgc agattactta cgccagtttg
     8821 atttgcttgt cctgaatttt atctctcgcg gtaattttgt catactcccc cgattatgga
     8881 ataactctga ggttcacaga tggttcgtca ataaagatcc taacttgatc accgccattc
     8941 tcgacataac ggacagcgaa ttaaaagagg atttattgca aagcctgatg gattcattag
     9001 gttctaacaa acatgtacta cccgaagtct gcatctgctt tttattcctt ttagcagaac
     9061 aagaatctcc tcattttcag gacttgtttt tatttttcgc caatatgtta ttgcactatc
     9121 accaatttat gaatcccaat gaaagtgatt tgaatgacgt gttgatgcca gcatcattaa
     9181 gtgatgataa aattatcaag catatggcgc gcaggaccct cgaactgttt gtaaaaaatg
     9241 aaacaccgcc aaaagttact cacgaagacc tggtgaaaaa caggcctcgc tcccctgtca
     9301 gaccacctat acccgcaacc tccaaaacgc cagacctccc tgaacgtcat taaaccgtga
     9361 tgttaccgac tctctgacgc ggaacagaat cagcgtcaga gaaacggaaa acgcgatcca
     9421 gatcacaaat acattgtatt cacatcatta accgttttaa gatcatttca tcactttttc
     9481 gcaactcacc cgataatctg ttatgacaac aaacactgtt tcccgcaaag tggcgtggct
     9541 acgggtcgtt acgctggcag tcgccgcctt catcttcaac accaccgaat ttgtccctgt
     9601 tggcctgctc tctgacattg cgcaaagttt tcacatgcaa accgctcagg tcggcatcat
     9661 gttgaccatt tacgcatggg tagtagcgct aatgtcattg ccttttatgt taatgaccag
     9721 tcaggttgaa cggcgcaaat tactgatctg cctgtttgtg gtgtttattg ccagccacgt
     9781 actgtcgttt ttatcgtgga gctttaccgt tctggtgatc agtcgcattg gtgtggcttt
     9841 tgcacatgcg attttctggt cgattacggc gtctctggcg atccgtatgg ctccggccgg
     9901 gaagcgggca caggccttga gtttaattgc caccggtaca gcactggcga tggtcttagg
     9961 tttacctctc gggcgcattg tggggcagta tttcggttgg cgaatgacct tcttcgcgat
    10021 tggtattggg gcgcttatca cccttttgtg cctgattaag ttacttccct tactgcccag
    10081 tgagcattcc ggttcattga aaagcctccc gctattgttc cgccgcccgg cattgatgag
    10141 catttatttg ttaactgtgg tggttgtcac cgcccattac acggcataca gctatatcga
    10201 gccttttgtg caaaacattg cgggattcag cgccaacttt gccacggcat tactgttatt
    10261 actcggtggt gcgggcatta ttggcagcgt gattttcggt aaactgggta atcagtatgc
    10321 gtctgcgttg gtgagtacgg cgattgcgct gttgctggtg tgtctggcac tgctgctacc
    10381 tgcggcgaac agtgaaatac acctcggggt gctgagtatt ttctggggga tcgcgatgat
    10441 gatcatcggg cttggtatgc aggttaaagt gctggcgctg gcaccagatg ctaccgacgt
    10501 cgcgatggcg ctattctccg gcatatttaa tattggaatc ggggcgggtg cgttggtagg
    10561 taatcaggtg agtttgcact ggtcaatgtc gatgattggt tatgtgggcg cggtgcctgc
    10621 ttttgccgcg ttaatatggt caatcattat atttcgccgc tggccagtga cactcgaaga
    10681 acagacgcaa tagttgaaag gcccattcgg gcctttttta atggtacgtt ttaatgattt
    10741 ccaggatgcc gttaataata aactgcacac ccatacatac cagcaggaat cccatcagac
    10801 gggagatcgc ttcaatgcca cccttgccca ccagccgcat aattgcgccg gagctgcgta
    10861 ggcttcccca caaaataacc gccaccagga aaaagatcag cggcggcgca accatcagta
    10921 cccaatcagc gaaggttgaa ctctgacgca ctgtggacgc cgagctaata atcatcgcta
    10981 tggttcccgg accagcggta cttggcattg ctagcggcac aaaggcaata ttggcactgg
    11041 gttcatcttc cagctcttcc gacttgcttt tcgcctccgg tgaatcaatc gctttctgtt
    11101 gcggaaagag catccgaaaa ccgataaacg cgacgattaa gccgcctgca attcgcagac
    11161 cgggaatcga aatgccaaat gtatccatca ccagttgccc ggcgtaatac gccaccatca
    11221 tgatggcaaa tacgtacacc gaggccatca acgactgacg attacgttcg gcactgttca
    11281 tgttgcctgc caggccaaga aatagcgcga cagttgttaa tgggttagct aacggcagca
    11341 acaccaccag ccccaggcca attgctttaa acaaatctaa cattggtggt tgttatcctg
    11401 tgtatctggg ttatcagcga aaagtataag gggtaaacaa ggataaagtg tcactcttta
    11461 gctaaccttg catcgcattg accaaaactt gaaccgattt agcaaaacgt ggcatcggtc
    11521 aattcattca tttgacttat acttgcctgg gcaatattat cctctgcaac taattacttg
    11581 ccagggcaac taatgtgaaa agtaccagcg atctgttcaa tgaaattatt ccattgggtc
    11641 gcttaatcca tatggttaat cagaagaaag atcgtctgct taacgagtat ctgtctccgc
    11701 tggatattac cgcggcacag tttaaggtgc tctgctctat ccgctgcgcg gcgtgtatta
    11761 ctccggttga actgaaaaaa gtgttgtcgg tcgacctggg agcactgacc cgtatgctgg
    11821 atcgcctggt ctgtaaaggc tgggttgaaa ggttgccgaa cccgaatgat aagcgcggcg
    11881 tactggtaaa acttaccacc agcggcgcgg caatatgcga acaatgccat caattagttg
    11941 gccaggacct gcatcaagaa ttaacaaaaa acctgacggc ggacgaagtt gcaacacttg
    12001 agcatttgct taagaaagtc ctgccgtaaa caaaaaagag gtatgacgat gtccagacgc
    12061 aatactgacg ctattaccat tcatagcatt ttggactgga tcgaggacaa cctggaatcg
    12121 ccactgtcac tggagaaagt gtcagagcgt tcgggttact ccaaatggca cctgcaacgg
    12181 atgtttaaaa aagaaaccgg tcattcatta ggccaataca tccgcagccg taagatgacg
    12241 gaaatcgcgc aaaagctgaa ggaaagtaac gagccgatac tctatctggc agaacgatat
    12301 ggcttcgagt cgcaacaaac tctgacccga accttcaaaa attactttga tgttccgccg
    12361 cataaatacc ggatgaccaa tatgcagggc gaatcgcgct ttttacatcc attaaatcat
    12421 tacaatagct agttgataac gtgacaacgt cactgaggca atcatgaaac cactttcatc
    12481 cgcaatagca gctgcgctta ttctcttttc cgcgcagggc gttgcggaac aaaccacgca
    12541 gccagttgtt acttcttgtg ccaatgtcgt ggttgttccc ccatcgcagg aacaaccacc
    12601 gtttgattta aatcacatgg gtactggcag tgataagtcg gatgcgctcg gcgtgcccta
    12661 ttataatcaa cacgctatgt agttattgtc aggctctgat cacagagcct gacatctccg
    12721 taatatcttc ctcaagtctc aaatattttc gccattgttg gcgatgagat cccgatacca
    12781 gtaaaacgac gctttacgta tccgttccag cgttcccttc ccttcgttgt ctttatcgac
    12841 ataaatcatt ccgtagcgct ttttcatttc ccctgttccg gcagaaacca ggtcaatgca
    12901 gccccacggg gtgtagccaa ttaagtcaac accatcttca actacggctt ttttcatttc
    12961 ccgaatatgg gaagcaaagt aatcaatgcg atagtgatcg ttcaccgtgc cgtcagcttg
    13021 tctctggtca accgcaccaa atccattttc gacaataaac agcggcaact ggaaatgatc
    13081 ccagaaccag ttgagtgaat aacgtagccc tgccggatca atttgccatc cccattcgga
    13141 tgtatcgata taagggttgc tgaccaaatc tcgtgattca acataatcca gttgcgggtt
    13201 atcgggagaa aattgtgtcg taaaagacat gtagtagcta aaaccgataa agtcgacaca
    13261 tccactggca agaatcgcgt tatcttctgg tgtgatatcg aggttgaatc ctttcctggc
    13321 aaagtaattc agcatatgtt gcggataata tccacgagca tgaacatcag taaaccagta
    13381 acgacgatgc atcgctttcg tggccatcat catatcgttg ggtgcacacg tcagaggata
    13441 gatggggcac atggcgatca tacagccaat gttaaattca ggattgatcg attttccagt
    13501 ctgtaccgcc agggcactgg caactaactc gtaatgtacc gcctggtaca ttatttgttc
    13561 gcgctcattg atatcttcct ctggcgaata caagataccg gaattagtaa atggacacag
    13621 gctttcgctg aaattcacct gattattgat ttcgttaaac gtcatccagt actttacttt
    13681 ttctttatag cgcgtgaaga ccgttgatgc gaagcggata aaaaagtcga tcagcttacg
    13741 gtttcgccag ccaccatatt ttgtcaccag atgataaggc atctcaaaat gcgaaagcgt
    13801 caccacaggt tccattcctt gcttcaggca ttcatcgaac agatcatcat aaaattgtaa
    13861 accctcttca ttcggctcct gttcgtcacc ttgcggaaag attcgtgtcc aggcaatgga
    13921 agttcgaaag catttgaatc ccatctcggc aaataactga atatctgttt tatagcgatg
    13981 ataaaaatca attgcttcat gattgggata attaagcccg tcgataacgc cttctgtcac
    14041 ttcacgcggc accccgtgag cgccagcagt catcacatca gcgatactga tgccttttcc
    14101 tccttcattc cagccacctt ccaactgatg cgcggctacc gcgccacccc ataaaaaacc
    14161 ttttttaaat cctgacatac acaactcctt aactaaaata gattaccacc agacttc
//
LOCUS       DAZPDU010000112        13962 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZPDU010000112 DAZPDU010000000
VERSION     DAZPDU010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13962
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.113"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            73..2214
                     /gene="ldcC"
                     /locus_tag="ACHURS_002960"
     CDS             73..2214
                     /gene="ldcC"
                     /locus_tag="ACHURS_002960"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFR1238632.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            2271..2660
                     /locus_tag="ACHURS_002961"
     CDS             2271..2660
                     /locus_tag="ACHURS_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR1238633.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            2725..4023
                     /gene="tilS"
                     /locus_tag="ACHURS_002962"
     CDS             2725..4023
                     /gene="tilS"
                     /locus_tag="ACHURS_002962"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFR1238634.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVRHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRFGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(4072..4332)
                     /gene="rof"
                     /locus_tag="ACHURS_002963"
     CDS             complement(4072..4332)
                     /gene="rof"
                     /locus_tag="ACHURS_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFR1238635.1"
                     /translation="MSMNDAYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(4319..4519)
                     /locus_tag="ACHURS_002964"
     CDS             complement(4319..4519)
                     /locus_tag="ACHURS_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFR1238636.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            4685..5230
                     /locus_tag="ACHURS_002965"
     CDS             4685..5230
                     /locus_tag="ACHURS_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFR1238637.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADYEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAKV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            5227..5588
                     /gene="arfB"
                     /locus_tag="ACHURS_002966"
                     /pseudo
     CDS             5227..5588
                     /gene="arfB"
                     /locus_tag="ACHURS_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
     gene            5662..6372
                     /gene="nlpE"
                     /locus_tag="ACHURS_002967"
                     /gene_synonym="cutF"
     CDS             5662..6372
                     /gene="nlpE"
                     /locus_tag="ACHURS_002967"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFR1238638.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(6527..7351)
                     /locus_tag="ACHURS_002968"
     CDS             complement(6527..7351)
                     /locus_tag="ACHURS_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFR1238639.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITVPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSSYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRTGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(7404..9122)
                     /gene="proS"
                     /locus_tag="ACHURS_002969"
     CDS             complement(7404..9122)
                     /gene="proS"
                     /locus_tag="ACHURS_002969"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFR1238640.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(9233..9940)
                     /gene="tsaA"
                     /locus_tag="ACHURS_002970"
     CDS             complement(9233..9940)
                     /gene="tsaA"
                     /locus_tag="ACHURS_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFR1238641.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(9937..10341)
                     /gene="rcsF"
                     /locus_tag="ACHURS_002971"
     CDS             complement(9937..10341)
                     /gene="rcsF"
                     /locus_tag="ACHURS_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFR1238642.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTLGCYRQAVCIGSALNITAK"
     gene            complement(10459..11274)
                     /gene="metQ"
                     /locus_tag="ACHURS_002972"
     CDS             complement(10459..11274)
                     /gene="metQ"
                     /locus_tag="ACHURS_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFR1238643.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(11314..11967)
                     /locus_tag="ACHURS_002973"
     CDS             complement(11314..11967)
                     /locus_tag="ACHURS_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFR1238644.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(11960..12991)
                     /gene="metN"
                     /locus_tag="ACHURS_002974"
     CDS             complement(11960..12991)
                     /gene="metN"
                     /locus_tag="ACHURS_002974"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFR1238645.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            13179..13754
                     /gene="gmhB"
                     /locus_tag="ACHURS_002975"
     CDS             13179..13754
                     /gene="gmhB"
                     /locus_tag="ACHURS_002975"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFR1238646.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT         3389 a         3535 c         3712 g         3326 t
ORIGIN      
        1 cttcggtggc ccttttttat cgccacggtt tgagcaggct atgattaagg aaggattttc
       61 caggaggaac acatgaacat cattgccatt atgggaccgc atggcgtctt ttataaagat
      121 gagcccatca aagaactgga gtcggcgctg gtggcgcaag gctttcagat tatctggcca
      181 caaaacagcg ttgatttgct gaaatttatc gaacataacc ctcgaatttg tggcgtgatt
      241 tttgactggg atgagtacag tctcgattta tgcagcgata tcaatcaact taatgaatat
      301 ctcccgcttt atgccttcat caacacccac tcgacgatgg atgtcagcgt gcaggatatg
      361 cggatggcgc tctggttttt tgaatatgcg ctggggcagg cggaagatat cgccattcgt
      421 atgcgtcagt acaccaacga atatcttgat aacattacgc cgccgttcac gaaagccttg
      481 tttacctacg tcaaagagcg gaagtacacc ttttgtacgc cggggcatat gggcggcacc
      541 gcatatcaaa aaagcccggt tggctgcctg ttttatgatt ttttcggcgg gaataccctc
      601 aaggctgatg tctctatttc ggtcaccgag cttggttcgt tgctcgacca caccgggcca
      661 cacctggaag cggaagagta catcgcgcgg acttttggcg cggaacagag ttatatcgtt
      721 accaacggaa catcgacgtc gaacaaaatt gtgggtatgt acgccgcgcc atccggcagt
      781 acgctgttga tcgaccgcaa ttgtcataaa tcgctggcgc atctgttgat gatgaacgat
      841 gtagtgccag tctggctgaa accgacgcgt aatgcgttgg ggattcttgg tgggatcccg
      901 cgccgtgaat ttactcgcga cagcatcgaa gagaaagtcg ctgccaccac gcaggcacaa
      961 tggccggttc atgcggtgat taccaactcc acctatgatg gcttgctcta caacaccgac
     1021 tggatcaaac agacgctgga tgtcccgtcg atccacttcg attccgcctg ggtgccgtac
     1081 acccattttc atccgatcta ccaggggaaa agtggtatga gcggcgagcg tgttgcggga
     1141 aaagtgatct tcgaaacgca atcgacccac aaaatgctgg cggcgttatc gcaggcttcg
     1201 ctgatccaca ttaaaggcga gtatgacgaa gaggcgttta acgaagcctt tatgatgcat
     1261 accaccacct cgcccagtta tcccattgtt gcttcggttg agacggcggc ggcgatgctg
     1321 cgtggtaatc cgggcaaacg gctgattaac cgttcggtgg agcgggcgct gcattttcgc
     1381 aaagaggtcc agcggttgcg ggaagagtct gacggttggt ttttcgatat ctggcaaccg
     1441 ccgcaggtgg atgaagccga atgctggccc gttgcgccag gcgaacagtg gcacggcttt
     1501 aacgatgcgg atgccgatca tatgtttctc gatccggtta aagtcactat tttgacaccg
     1561 gggatggacg aacagggcaa tatgagcgag gaggggatcc cggcggcgct ggtggcaaaa
     1621 ttcctcgacg aacgtgggat cgtagtagag aaaaccggcc cttataacct gctgtttctc
     1681 tttagtattg gtatcgataa aaccaaagca atgggattat tgcgtgggtt gacggaattt
     1741 aaacgctctt acgatctcaa cctgcggatc aaaaatatgc tacccgatct ctatgctgaa
     1801 gatcccgatt tctaccgcaa tatgcgtatt caggatctgg cgcaagggat ccataagctg
     1861 attcgtaaac acgatcttcc cggtttgatg ttgcgggcat tcgatacttt gccggaaatg
     1921 atcatgacgc cacatcaggc atggcagaga cagattaaag gtgaagtaga aaccattgcg
     1981 ctggaacaac tggtcggtag agtatcggca aatatgatcc tgccttatcc accgggcgta
     2041 ccgctgttga tgccaggaga aatgctgacc aaagagagcc gcacggtact cgattttcta
     2101 ctgatgcttt gttccgtagg gcaacattac cccggttttg aaacggatat tcacggcgcg
     2161 aaacaggacg aagacggcgt ttaccgcgta cgagtcctaa aaatggcggg ataacttgcc
     2221 agagcggctt ccgggcgagt aacgtgctgt taacaaataa aggagacgtt atgctgggtt
     2281 taaaacaggt tcaccatatt gcgattattg cgacggatta tgcggtgagc aaagctttct
     2341 actgcgatat tcttggtttc acgctgcaaa gcgaagtcta tcgcgaagcg cgcgactcgt
     2401 ggaaagggga tttggcgctt aatgggcaat atgtgattga gcttttctct tttccgttcc
     2461 cgccggagcg ccccagccga ccggaagctt gcggcctgcg tcatctggct tttagcgttg
     2521 atgatatcga tgcggcagtg gcgcaccttg aaagccataa cgtgaagtgt gaagccatcc
     2581 gtgtcgatcc atacacgcaa aaacgcttca cgttctttaa cgatccggac gggctgccgt
     2641 tggaactgta tgagcagtaa ggcttgtcat cgccgcattt gcccggtaac gtgccgggca
     2701 ttgctactgt aaaatcgcac catcatgaca ctcacgctca atagacaact tctcacctca
     2761 cgccagattc tggtggcctt tagcggcggg cttgactcca ccgttctgct gcatcagttg
     2821 gtgcagtggc ggacggaaaa tccgggcgtc actctacgcg ctatccatgt gcatcatggt
     2881 ttaagtgcta atgccgatgc ctgggttagg cattgcgaga acatctgcca acagtggcag
     2941 gtgccgctgg tggtcgagcg cgtgcaactt gcgcaagaag gactgggcat tgaggcccag
     3001 gcgcggcagg cgcgttatca ggcatttgct cgtaccctgt tacccggtga agtgctggtc
     3061 accgcgcaac atctcgacga tcaatgtgaa acctttctgc tggcgctaaa acgcggtagc
     3121 ggccctgccg ggctttcggc tatgggggaa gtttcggaat ttgccggaac acggcttatc
     3181 cgcccgttgc ttgcccgcac gcggggggaa ctggagcagt gggcattggc gcatggttta
     3241 cgctggattg aagacgaaag taatcaggac gactcatacg atcgtaactt tctgcgcctg
     3301 cgtgtagtgc cgttattgca gcagcgttgg ccgcattttg ccgaagcaac ggcccgcagc
     3361 gccgcacttt gtgctgaaca agagagtctg ttggatgaac tgctggcgga tgatttagca
     3421 cactgccaaa cgtcgcaggg gacgctgcag attgcgccaa tgctggcgat gagtgatgcc
     3481 cgtcgcgcgg cgattatccg ccgctggctg gcagggcaga atgcaccgat gccttcccgc
     3541 gacgcgctgg tgcggatctg gcaggaagtg gcgctggcgc gggaagatgc ctccccctgt
     3601 ttacgttttg gcgcgtttga aatacggcgc tatcagtcgc aactgtggtg gattaaatcc
     3661 gtcactgggc aaagcgaaac cattgtgccg tggcagacgt ggctacaacc gctggaatta
     3721 ccggcggggc tgggaagtgt acagcttact gcgggaggcg atattcgccc tccgcgtgca
     3781 gacgaagcgg tcagcgtgcg tttcaaagcg ccaggattgc tgcatattgt cgggcgtaac
     3841 ggcggacgta agctaaagaa aatctggcaa gagctgggcg tgccgccgtg gctacgtgac
     3901 accacgccac tgctgtttta tggcgaaacg ctgattgcgg cggcaggggt atttgtgacg
     3961 caagaaggtg tggctgaagg tgagaatggc gtaagttttg tctggcagaa aacgcttagt
     4021 taagtgaaag ccggataaga cgcatcaaac gtcgcatccg gcgaaagtca atcaggattc
     4081 gcttaccacc accgtaccga tttccgggtg gctaaagctg gtaattttat ccagacgcag
     4141 ctcgcgggtt tcgccagcgg cctcgacgac cagatactcc acatttttgc gggagactaa
     4201 atcactggct tttgcctgca atttttcgcc atctttcagc tcaagtgtca gcattaaatg
     4261 atgctggcag gcgagctcaa gattatcgta atcatcacaa ttgattggtt gatacgcatc
     4321 attcattgac ataatcgctc accagtaagt ttgccgcagc gtatgctgct ttttccctga
     4381 cagcctcaga aagggcgtcg tcggcagcca tttcattcag cactttcaaa acgcagccca
     4441 gcgcgtccgg aacgtatcct aagtctccgc tggcgatttc cgcgtaccgc ttgcgtatta
     4501 actcacaata tttttccaca tgccctcctg tcagcactct gacttaaccg tggatgcaag
     4561 tctaagccta cgaagataaa ctctgtttcg caaggtgact ataccacact catttctgca
     4621 atatcagcgc cgcaactgca cgtattccgt tacaatggcc tcctgattcg aaaggagttt
     4681 tcttatggcg cttaaagcga caatttataa agcgacggtt aatgtggccg atctcgaccg
     4741 caaccagttt ctcgatgcct ctctgacgct ggcgcgccac ccttcagaaa cccaggagcg
     4801 tatgatgctg cgcctgctgg cgtggctgaa atatgctgat gaacgtctgc aatttacccg
     4861 tggtttgtgt gccgattatg agccggaagc gtggctgcgt aacgatcacc tgggcattga
     4921 tttgtggatt gagctggggc tgccggatga gcggcggatt aagaaagcct gcacccaggc
     4981 cgcaaaagtg gcgctgttta cctataatag ccgggcggcg caaatctggt ggcagcaaaa
     5041 tcagagcaaa tgtgtgcagt ttgccaatct ttccgtctgg tatctggacg atgaacaact
     5101 ggcgaaagta agcgcctttg ccgatcgtac catgacgctg caggcaacga ttcaggatgg
     5161 cgtgatctgg ttatcggatg ataagaataa tctggaagtg aacttaaccg cctggcaaca
     5221 accttcatga ttgtgatttc ccgacatgtt gctattcccg atggtgagct tgagatcacc
     5281 gccattcgtg cgcagggcgc gggcgggcag catgttaata agacctcaac ggctattcat
     5341 ctgcgttttg acattcgggc gtccagcctg ccagagtatt acaaagagcg tctgctcgcc
     5401 gccagccatc atttgatcag catgatggcg taattgtcat taaggcacag gaataccgca
     5461 gtcaggaact gaaccgcgaa gcggcgctgg cccggctggt ggcagtgatt aaagatttaa
     5521 caacagaaca aaaagcccga cgacccacgc ggcccacccg tgcatcgaaa gagcgcaggc
     5581 tggcatagaa agcacaaaaa tcaagcgtga aggcgatgcg cggcaaagta cgcagcggtc
     5641 gggaataaaa agaaggaatg gatggtgaaa aaagcgatag tgacagcgat ggctgtaatc
     5701 agcctcttta ctctgatggg atgtaataat cgggccgaag tcgatacgct ttctccggcg
     5761 caggctgccg aactaaaacc gatgccgcaa agttggcgcg gcgtgctgcc gtgtgccgat
     5821 tgcgaaggga tcgaaacctc tctgttcctc gaaaaagacg gaacatgggt gatgaatgag
     5881 cgttatctcg gtgctcgtga agaaccttcc tccttcgctt cctacggtac atgggcgcga
     5941 accgctgaca agctggtatt aaccgacagc aaaggtgaaa agtcatatta tcgggcgaaa
     6001 ggcgatgcgc tggagatgct cgatcgtgaa ggcaatccaa ttgaatcgca gttcaactat
     6061 acgctggaac cggcacaatc cagtttacct atgacgccga tgaccctgcg aggcatgtat
     6121 ttttatatgg ctgatgcggc gaccttcact gattgcgcga ccggaaaacg tttcatggta
     6181 gcgaataacg cagagctgga gcgtagctac ctggctgcgc gcggtcacag tgaaaaaccg
     6241 gtgttactgt cagtagaagg tcactttacg cttgaggcta atccggatac tggtgcgcca
     6301 accaaagtat tagcgcccga tacggcgggt aaattttacc cgaataagga ttgcagtagt
     6361 ttggggcagt aaacctgctt aaggcagaaa caaacgcaaa attgcctgat gcgctgcgct
     6421 tatcaggcct acagcatata atgcaacatt ttgaattagc acgattttgt aggccggata
     6481 aggcgttcac gacgcatccg gcattttgtg cacgaggctg gctttatcac tgagtcagcc
     6541 caacaaagcg acccgccttg atataaatcc ctggcttcag gtgcccaaca tactgtaact
     6601 gcgtttctgg cttaaacgca gagacatcgc cagtacgcat atgcatcaga tcggcagggc
     6661 taatccagcc tgattgcgcc agcgtaagcg gatgcccggc tttggcaaat gcatcagtga
     6721 caaattccga acaaaaccac gactttttgt ctccttcgcc cacactgctt aactgcgctt
     6781 tcgccaggcc gctgacgcac tgttggcgaa aatcctcgga gaacggattc agtgagcaca
     6841 tctggcgagt caccataaag ggaataaatt cgacaatgcc gcgatagtta taactgctat
     6901 ctttgatttt attggcaaac gcggtgatat ctgtggcttg ttgcggggta agatccggga
     6961 ctcgtaagac gaaaagctta tcactatgct tcatcgcttt tttaagggaa acgatctgga
     7021 cgccagcgcc tgtcgcttct gcaacgttat tatcacccag atagattgca acgtgactca
     7081 cagaggaagt gctgaagacg cggattccaa atgaggttac cccaaggctt gaggagaaca
     7141 gcaaatcgcc gggtttgaga tcaggtaccg ttatttcttt tattgattgt tcggtgaaag
     7201 agctttgatg ctggaatttg acagcccatg tttttgcctc ggcatctact gctgtggctg
     7261 atgggtctgg ctggctgata tcaacggtac aagccgaaag taaaagaaag ctggggagaa
     7321 gcaggcggca gtacgccttt ggtttatcca ttttatacaa tccatgtaaa aaagggccct
     7381 gaaattcagg accctttctg gcatcagcct ttaatctgtt tcaccagata ttcgacgatg
     7441 tcaccagtct taattaactg tttctcgccg ttgcgacgat atttatattc gatatcgtcg
     7501 ttgtcgaggt tacggtcgcc cagcacaata gtgtgcggaa taccgatcag ttccatatca
     7561 gcaaacatca cgcccggacg ctctttgcgg tcatccagca gcacttcgat accttgtgca
     7621 cgcagttcgc tgtacagttt ctcagcaagc tcttgtacgc ggaaggattt gtgcatgttc
     7681 atcggcagaa tcgccacctg gaacggcgcg atagcgtcag gccatacgat gccgcgttcg
     7741 tcgtagttct gctcaatcgc cgcagctacc acacgcgtta ccccgatacc gtagcaaccc
     7801 atcgtcagga tttggttacg gccatcttca ccctgtacgg aggctttcag tgcttcggag
     7861 tacttggtac ccagctggaa gatgtgacca acttcgatac cacgtttgat cagcagcgta
     7921 ccctggccat ccgggcttgg atcgccagcc accacgttac ggatatctgc aacttccggg
     7981 gtagcgacat cgcgatccca gttgataccg aagtagtgtt taccatcgat gttagcacca
     8041 gcagcgaaat cactcatcgc cgcaacggta cggtcaatca ccaccggaat cggcatgttt
     8101 accggaccca gtgaacccgg accggcttta accacagcac gaatttcttc ttcggtcgcg
     8161 aaagtcagcg ggcttgcaac ctgcggcagt ttttctgctt taacttcgtt cagctcgtga
     8221 tcaccgcgca ccagcagcgc aaccagcggg aagctgctgc cttcaaccgc tttaaccagc
     8281 agagtcttaa ccgttttctc aatcggcaga ttgaactgtt caaccagttc cgcgatggtt
     8341 ttcgcgttcg gcgtatcaac cagcgtcatt tcctgggtag cagcagcgcg cggttctttc
     8401 ggcgcgatag cttctgccag ttcaatgttc gctgcatagt cagaggtgtc ggagaagacc
     8461 acatcgtctt caccgctctg cgccagcacc tggaattcgt gagaggcgct gccgccgata
     8521 gaaccggtgt cggcttgcac ggcgcggaaa tccagcccca tgcggctgaa gattttgctg
     8581 taggccgcat acattgcatc gtaggtttcc tgcagggatt cctgagaagt atggaaagag
     8641 taagcatctt tcatcaggaa ttcgcgggaa cgcatgacgc cgaaacgcgg acgcacttcg
     8701 tcgcggaact tggtctggat ctgatagaag ttcagcggca gctgtttgta agagctaagc
     8761 tcgttacgaa tcaggtcagt gataacttct tcatgagttg ggccgagtac gaacggacgc
     8821 tcgccacggt caacaaaacg cagcagttcc ggaccgtact gttcccaacg accactctct
     8881 tgccacaaat cggctggctg aaccaccggc atcgacacct cgattgcacc ggcgttgttc
     8941 atctcttcac gcacgatgtt ttcgactttt ttcagaacgc gcacgccggt cggcagccag
     9001 gtatataacc cggaggccag cttgcggatc atcccggcgc gcagcatcag ctgatggctg
     9061 atcacctcgg cgtcggcagg tgtctccttg agagtggaga gcaggtattg gctagtacgc
     9121 atgttgttac ggttccagtt ggaaggtaga acaggctcaa ggcgagcctg ggacaaaaaa
     9181 agtgatttag tttaccagtg caaaagaaat gtcaaaagag aagggcgtga atttaacgcg
     9241 gttccagcgc aaagacttca aaacctgcgt cggtgacgcg ccagcgaacg ttaaaatcat
     9301 gtagccagac ggcataggtt ttgcccgttt cctcaccttt acgataggcc gggcgcgggt
     9361 cctgcgccag tacttcgcgg ataaacagcg ttaactgcgg ataacgcttc tccagcgtca
     9421 aaagctgctt ttcgacctct gcggtaaaac tcaccgccat ctctgcagct ggcgcacttt
     9481 gcgcatagct ggcactggca tcgggaagcg attcggcaaa ggggagatac ggtttgatat
     9541 ccactaccgg cgtaccatcg accagatcca gactgccgag cttcagaatc acgctgtctt
     9601 tatggcaaac aacctctttc agctctacca gcgacatacc aattgggtta gggcggaaag
     9661 tagagcgtgt tgcgaaaacc cccattctgg cgttaccgcc gaggcgcggt ggacgcacag
     9721 tcggacgcca gccgccttcc atcgtttgat gaaagacgaa aaggatccat aaatggctga
     9781 acgcttccag gccgcgaacg gcgtcggcct ggttgtaggg agcaatgaga tgcagttcgc
     9841 cgttggcgct ttttaccaga ccaggctggc gcggaacggc gaatttttct ttatagggcg
     9901 agcgaataac gcctatttgc tcgaactgga aactgctcat ttcgccgtaa tgttaagcgc
     9961 agagccgata catacagcct gacgatagca gcctagcgta ccgctggtga cttcgcagct
    10021 atgcagtaat accgcattgg ctttcatttt agaggcgttg atttgcatcc gcttacgcgc
    10081 ggttggaatg ctcggcggag agtcctgatt agaggcctgg caagagtcgc cactgacttc
    10141 accgagatcg cggaacggtt tgccgactaa ttcttctgca ttggtataaa ttcggaccgg
    10201 cgtggcgcgc ggcgctttcg gttttgcagg ctccgctttc ggctggggtg cagtgctttg
    10261 aacgggttcg acaggggatc tgcttaacat ggaacagccg cttagcatga gtgctactaa
    10321 acagatcggt aaagcacgca tagtatttcc tcaatgtatg atcaaaacgt caatattgaa
    10381 tcaggagctt gtaaaaatga caagacgggc aagcgcccgt cctgaatgat attacaaatt
    10441 gtggaaacag cctaaaaatt accagccttt aacagcgccg ccgttaaaca ctttgtttgc
    10501 tgcttcgtaa acttcatcag actgataagc ctggacgaat ttcttcacgt tctcggcgtc
    10561 tttgttatct tcacgcgtta cgatcaggtt cacgtacggg gagtctttat cttcgacaaa
    10621 gataccgtct ttcgctggag tcaggccaat ctggctggca taggtggtat tgataactgc
    10681 cagagcgatt tgcgcgtcgt ccagagatcg aggcagctgc ggtgcttcca gctcaacaat
    10741 tttcagattt tttgggttct caacaacatc aagaacggtc ggcagcaggc caacgccatc
    10801 tttcagtttg atcaagccca ctttttgcag cagcagcagt gaacgaccaa gattagttgg
    10861 atcgtttggc acggcaacct gcgaaccatc ctgcagttca tccagtgatt tgattttctt
    10921 ggagtaacca gcaatcggat aaacaaaagt gttgcctact gcgaccagtt tgtagccacg
    10981 atctttcagt tgctgatcaa ggtacggttt atgctggaag gcgttggcgt cgatatcgcc
    11041 tttgctcaat gcttcgtttg gcagaacata gtcgttgaag gttaccagct caacgtccag
    11101 gccatatttg tctttcgcaa ctttctgcgc gacttctgct acctgctgtt cggcaccaac
    11161 aatcacgccg actttaatgt ggtttggatc tttttcatcc tgaccgcagc ctaccagtgc
    11221 cagtgatcca atcagggctc ccactgccgc aaaggttttg aatttgaacg ccatacctta
    11281 ttccttcttc aattatttat gttgtgttga acgttacttg cgagtgacag cccggacgat
    11341 gcggtcgcct gcgaactgaa ttaaataaac cagaatgacc agcaatacca gtaccgtatt
    11401 catcaccgtc gcgttgtagc cgatgtagcc atactgatag ccaatctgac ctaaaccacc
    11461 ggcaccgact gcaccaccca tcgcggaata accgaccagg gtaatcaggg tgatagttgc
    11521 cgcattcacc agacccggca acgcttccgg taacagcact ttacggacga tctgcattgg
    11581 cgtggcaccc attgcgcggg aagcttcaat taacccggtt gggatctcca gcagagcgtt
    11641 ctcgaccata cgggcaataa acggtgctgc accaacggtt aacggaacaa ttgctgcctg
    11701 caaaccaatc gatgtaccga caataacgcg ggtaaacgga atcatccata ccagcaagat
    11761 aatgaacggg atggaacgga aaatgttcac aatcgcagaa acggtacgat acagcttcgc
    11821 gttagcaata atttgccccg gacgcgtgac ataaagcaga acgccaaccg gcagaccaat
    11881 cacaaagcca aaaaatccgg atacgaaggt cattgccagc gtttcccata cgccacgaac
    11941 cagcagccac atcatcggct cagacataac ccagtacctc tacttttaca tggtgttcct
    12001 gcagccaggc aatggcggct tgcgtatctt gttgtgtgcc gtgcatttca gtcagcatga
    12061 tgccgaattt aacgccaccg gcgtagtcca tctgcgcgct aataatgttg ttgttgacgt
    12121 tgaaacgacg cgcggtttca gaaagcagtg gggcatcgac cgattgaccg gtaaactcca
    12181 gacgcagcat cggcacgcag tcagtaaatg gctccgcttg cagacgttcc tggtaatctt
    12241 ccgggatatc cagatgcagg gtcgactgaa taaacttctg cgccagcggc gttttcggat
    12301 gcgagaacac ttcacttacc gtgtcctgct cgatcagttc gccattgctg atgaccgcca
    12361 cgcaatcaca aatacgcttc acaacgtcca tttcgtgggt gatcaacaga atcgtcaaac
    12421 ccagacggcg gttaatgtct ttcagcagtt cgagaataga acgtgtcgtt gccgggtcca
    12481 gcgcgctggt ggcttcatca cacagcaata ctttgggatt gctggctaat gcacgggcaa
    12541 tcgccacacg ttgtttctgc ccaccggaaa gattcgacgg gtagctatca tgcttatcgc
    12601 caagaccaac caatgacagc aattccgtca cgcgacgttt gatctcgtct ttcggtgtgt
    12661 tgtccagctc cagcggcaga gccacgttgc caaaaacagt acgcgaagag agcaggttaa
    12721 aatgctggaa aatcatacca atctggcggc gagctttggt caactcggat tctgacagcg
    12781 tggtcaattc ttggccatcg accagcacgc taccctcggt tgggcgctcc agcaggttta
    12841 cacaacgtat aagcgtactc ttacccgcgc ctgaggcacc gataacgcca taaatttgtc
    12901 cagctggcac atgcaggctg acgttgttca acgcctggat ggtgcgggtg ccctggtgga
    12961 acactttggt gatattcgaa agttttatca ttgattattt attatcgtca ttaagttagt
    13021 cgtggcatct cgaatgcctg aaacgggcaa cgccgtcaat gaaatggatg ttaaggcatc
    13081 cagacgtcta aatcaatcag gtttatgcga agagcacttt cttgcaggtc gaaacatgcg
    13141 atactagcgt cccatgcctt attaaggagc tataaaaggt ggcgaagagc gtacccgcaa
    13201 tttttcttga ccgtgatggc accattaatg tcgatcacgg ctatgtccat gagatcgaca
    13261 actttgaatt tatcgacggt gttattgacg ccatgcgcga gctaaaaaaa atgggctttg
    13321 cgctggtggt agtaaccaac cagtctggca ttgctcgcgg taaatttacc gaagcacagt
    13381 ttgaaacgct gaccgagtgg atggactggt cgctggcgga ccgagatgtc gatctggatg
    13441 gtatctatta ttgcccgcat catccgcagg gtagtgttga agagtttcgc caggtctgcg
    13501 attgccgcaa accacatccg gggatgcttt tgtcagcacg cgattatttg catattgata
    13561 tggccgcttc ttatatggtg ggcgataaat tagaagatat gcaggcagcg gttgcggcga
    13621 acgtgggaac aaaagtgctg gtgcgtacgg gtaaacctat tacacctgaa gcagaaaacg
    13681 cggcagattg ggtgttaaat agcctggcag acctgccgca agcgataaaa aagcagcaaa
    13741 aaccggcgca atgattaaaa gatgagcggt tgaaataaaa atgcattttt ccgcttgtct
    13801 tcctgagccg actccctata atgcgcctcc atcgacacgg cggatgtgaa tcacttcaca
    13861 caaacagccg gttcggttga agagaaaaaa tcctgaaatt cagggttgac tctgaaagag
    13921 gaaagcgtaa tatacgccac ctcgcgacag tgagctgaaa gc
//
LOCUS       DAZPDU010000113        13902 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZPDU010000113 DAZPDU010000000
VERSION     DAZPDU010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13902
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.114"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            241..1494
                     /gene="kch"
                     /locus_tag="ACHURS_002976"
     CDS             241..1494
                     /gene="kch"
                     /locus_tag="ACHURS_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFR1238647.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
     gene            complement(1549..1722)
                     /locus_tag="ACHURS_002977"
     CDS             complement(1549..1722)
                     /locus_tag="ACHURS_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFR1238648.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            1865..3325
                     /gene="cls"
                     /locus_tag="ACHURS_002978"
     CDS             1865..3325
                     /gene="cls"
                     /locus_tag="ACHURS_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFR1238649.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            3360..3689
                     /locus_tag="ACHURS_002979"
     CDS             3360..3689
                     /locus_tag="ACHURS_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFR1238650.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(3742..4746)
                     /gene="oppF"
                     /locus_tag="ACHURS_002980"
     CDS             complement(3742..4746)
                     /gene="oppF"
                     /locus_tag="ACHURS_002980"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFR1238651.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTFKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPISALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTCCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(4743..5756)
                     /gene="oppD"
                     /locus_tag="ACHURS_002981"
     CDS             complement(4743..5756)
                     /gene="oppD"
                     /locus_tag="ACHURS_002981"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFR1238652.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(5768..6676)
                     /gene="oppC"
                     /locus_tag="ACHURS_002982"
     CDS             complement(5768..6676)
                     /gene="oppC"
                     /locus_tag="ACHURS_002982"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFR1238653.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(6691..7611)
                     /gene="oppB"
                     /locus_tag="ACHURS_002983"
     CDS             complement(6691..7611)
                     /gene="oppB"
                     /locus_tag="ACHURS_002983"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFR1238654.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIVALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(7697..9328)
                     /gene="oppA"
                     /locus_tag="ACHURS_002984"
     CDS             complement(7697..9328)
                     /gene="oppA"
                     /locus_tag="ACHURS_002984"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFR1238655.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDSTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            9612..9908
                     /locus_tag="ACHURS_002985"
     CDS             9612..9908
                     /locus_tag="ACHURS_002985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1238656.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLSIQSNRDWL
                     SMSIFSDYSSSSEMHNNLTIDYYLALSSTKGSGITNIISIILQQAQDYDVAKIT"
     gene            complement(10066..10713)
                     /gene="ychE"
                     /locus_tag="ACHURS_002986"
     CDS             complement(10066..10713)
                     /gene="ychE"
                     /locus_tag="ACHURS_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFR1238657.1"
                     /translation="MIQTFLDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            11191..13866
                     /gene="adhE"
                     /locus_tag="ACHURS_002987"
                     /gene_synonym="adhC"
     CDS             11191..13866
                     /gene="adhE"
                     /locus_tag="ACHURS_002987"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFR1238658.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         3656 a         3436 c         3231 g         3579 t
ORIGIN      
        1 ttatagccgg tcagtgcatt cactaccaca agcagataat aactttatct gtatttcttt
       61 atatgatgtg agcatattca ggaaaaatga cctttcctga ggtacagttg ctcgctcaaa
      121 tcctttgatt actgcggata aagtcatcta ctattgttta acacattcac gcgctgcatg
      181 tatgttcaga aaatgtgcac gctaagataa ttaaacaaaa ttgtggttac agaggtatca
      241 gtgagtcact gggctacatt caaacaaaca gcaacaaatt tatgggtgac ccttcggcac
      301 gacattctcg cgctggccgt ctttttaaat ggattgctta tttttaaaac aatctatggt
      361 atgtcggtca atttgcttga tattttccat atcaaagcat tttcagagtt ggatctctcc
      421 ttgctggcaa acgccccact atttatgctc ggcgtctttc ttgtcctgaa ctccattggc
      481 ttactgttcc gggcaaagct cgcatgggca atcagtatca ttttgttgtt gatagcgcta
      541 atttacaccc tgcattttta tccctggctg aaatttagta ttggattttg catttttacg
      601 ctggtgtttt tgctgatact gcgcaaagac ttctcccaca gtagcgccgc agccgggaca
      661 atttttgcat ttattagttt cacgacatta ctgttttact caacctacgg tgcgctttat
      721 ttaagcgaag gttttaatcc gcgaatagaa agtttgatga ccgcgttcta tttttcgata
      781 gaaaccatgt caaccgtcgg ctatggcgat attgtccctg tttctgaatc agcacgattg
      841 ttcactattt cggtcattat ttccggcatt accgtttttg ccacatctat gacctcaatt
      901 tttggtccgc ttatccgcgg gggattcaac aaacttgtaa aaggaaacaa tcatacaatg
      961 catcgtaaag atcattttat tgtttgcgga cattcgattc tcgccatcaa tacgatcctg
     1021 caactgaatc aacgcggaca aaacgtaacg gttatcagca acttgcctga agatgatatc
     1081 aagcaacttg agcaacgctt aggcgataac gctgatgtta tccccggtga cagtaatgac
     1141 agttcagtat taaagaaagc gggaatcgat cgttgccggg ccattctggc gctgagtgat
     1201 aacgatgcag ataacgcgtt tgttgtactc tcggcaaaag atatgagcag tgatgtcaaa
     1261 acagttctcg ccgtcagtga tagcaaaaac ctgaataaga ttaagatggt acatccggat
     1321 atcattctct caccgcaact gtttggcagc gaaattctgg cgcgagtttt aaatggtgaa
     1381 gagattaata atgatatgct cgtttcaatg ttgttgaact ccggtcatgg cattttcagc
     1441 gataacgatg aacaagaaac gaaagctgac agtaaagaat cagtgcaaaa atagagttta
     1501 aagacgaaag agtaaactta ttgggcaccg caatggtgcc cagtatattt agatattgta
     1561 gatcgcaaac aataacgagt tacgctgttt gtttagaatg cacttcctga tgctgtggat
     1621 acgcatattt cggcgcgatt gcccctcaag ccaacgcgat ttacgtcggc tagcctgacg
     1681 ctgcatgcgc cagcgcccca cttccgttct actccgcttc atgttaacta ctctatgcaa
     1741 taacagaatg gtcattatac ccccaccccc gacgctgacc agtgcttttc ccgcgttttt
     1801 atttgccaga tgaatccata tgcgtaaact cataacaatg cgctttcaaa aggatttcta
     1861 atctatgaca accgtttata cgttggtgag ttggttggcc attctgggat actggttgct
     1921 cattgcaggc gtaactttac gcattctaat gaaacgacgc gcagttccct ccgcgatggc
     1981 ctggctgttg attatttaca ttctgccgtt agtcggaatt attgcctatc ttgccgttgg
     2041 cgagctccat ttaggcaaac gccgcgctga gcgcgccaga gcgatgtggc cttccaccgc
     2101 aaaatggctt aacgacctta aagcctgtaa gcatatcttc gccgaagaaa atagcagtgt
     2161 cgctgcgcca ttattcaagc tttgcgagcg tcgtcagggg atcgctgggg tcaaagggaa
     2221 tcagctacaa ctgatgaccg agtcagatga tgtgatgcag gcgttaatcc gcgacatcca
     2281 gctcgcgcgc cataatattg agatggtgtt ttatatctgg cagcccggcg gcatggcgga
     2341 ccaggtggct gaatcattaa tggcggctgc acggcgtggt attcattgcc gattgatgct
     2401 cgactccgcc gggagtgtgg cttttttccg cagcccgtgg cccgagctaa tgcgtaatgc
     2461 cggtattgaa gtggtcgaag ccttaaaggt caatctgatg cgtgtgtttt tacgccgtat
     2521 ggacctgcgc caacatcgca agatgatcat gatcgataat tacatcgcgt acaccggcag
     2581 catgaatatg gtcgatcctc gctacttcaa acaagatgcg ggcgtagggc aatggattga
     2641 tctgatggcg cgtatggaag gccccatcgc caccgcgatg gggattattt attcctgcga
     2701 ctgggagatt gaaaccggaa aacgtattct gccgccacca ccagatgtca atattatgcc
     2761 gtttgaacag gccagcggtc acaccattca cacaattgct tctggccccg gctttccgga
     2821 agatctcatt caccaggcat tattgactgc ggcttattcg gcgcgtgaat atttgatcat
     2881 gacaacgccc tactttgtgc caagcgatga tttacttcat gcgatttgca cggcggcgca
     2941 gcgcggggtg gatgtcagta ttatccttcc gcgaaaaaat gactcgatgc tggtcggctg
     3001 ggccagtcgc gcattcttta cggaactgct tgctgctggg gttaaaattt atcagtttga
     3061 aggcgggtta ctgcatacca agagcgtgct ggtcgatggc gaactaagtc tggttggcac
     3121 cgttaacctt gatatgcgta gtctgtggct gaatttcgag attaccctgg caatcgacga
     3181 taaaggtttt ggtgcagacc ttgccgccgt tcaggacgat tatatttcgc gttcacgtct
     3241 gctcgatgcc cgtttatggc taaaacgtcc attatggcaa cgtgtcgccg agcgactgtt
     3301 ttacttcttc agcccgttgc tgtaaaacgt cgccaacaga ggttaaacag gtagtgacta
     3361 tggatatgga tctaaacaat cgcctgactg aagatgaaac gcttgagcag gcctacgata
     3421 tttttctcga actggctgcc gacaatctcg acccagctga tgttctgctg ttcaatcttc
     3481 agtttgaaga gcgcggcggc gcggagttat tcgatccggc ggaagactgg caggaacatg
     3541 ttgatttcga tttgaacccg gacttttttg ccgaagtggt gattggtctg gcggacagtg
     3601 aggatggtga aatcaacgat gttttcgccc gcattctgtt atgccgcgaa aaagatcaca
     3661 aactttgcca tatcatctgg cgggaataaa taatcgcttt cggcgtgaaa aagggctgac
     3721 aactgtcagc ccttattgtg tttaaagcgg atcgactttc aggcaagaaa cggcgtgtct
     3781 gaaactccct tccagcacag gacgtgtttt ggcgcactcc ggaccggcaa tcgggcaaca
     3841 ggtacggaaa acacaaccgg aaggcgggtt gatcggcgac ggtaattccc cttccagtaa
     3901 ctggatggtt ttgttcttct ccagatccgg atcaggtatg gggactgccg acatcaatgc
     3961 cctggtgtaa ggatgtagtg gattgtggta gacctcatca taggtcccca gttctaccgc
     4021 atggccgaga tacatcacca acacacgatc ggaaatgtgt tttaccacgg ccaggtcatg
     4081 agcgataaaa attaatgaca atcccatctc acgttgcagc tgctggagca ggttgaccac
     4141 ctgcgcctga attgacacgt ccagcgccga aatcggctca tcgcagataa tcagcttcgg
     4201 ttcaagaata agagcacgag caatcccgat acgctggcac tgcccaccgg agaactcatg
     4261 cggatagcgg ttaatcaggt taggcaataa cccgactttc agcatcatcg ccttcacgcg
     4321 ctcgcgaact tcctggcgtg acattttcgg atgataagta cgcagtggtt cagcgatgat
     4381 ctcgccgatg gtcatacgcg ggtttagcga tgccaacgga tcctggaaaa tcatctgaat
     4441 atcactgcga acggcacgcc attcatcggg cttcatgccc agcaactctt tacctaacca
     4501 ggcaacatga ccgtcggtcg ctttaaccag gccaatgatg gcgcgagcaa aggtggactt
     4561 accgcatccc gattccccta ccacacctaa tgtttcccct tcatacaggc gaagcgtgac
     4621 accatcgacg gctttgaaag ttttcggcgg ttgccagaac cactgtttgc catctttgat
     4681 ttcaaagtgc actttcagat cggcgatttc gaggaggact tttcttcctt cggttacagc
     4741 attcataaca gttcctccac cggtttaaag caagcacgca gacggccagg cgtaaactct
     4801 tccagcggcg gtgcgctact acaaatttcc atcgcatgcg gacaacgtgg ctggaacggg
     4861 caacctttcg gtaatcgcag caggtttggc ggattaccag ggatggtcaa cattgtttca
     4921 ccttccgcat cgagacgcgg caccgcgttg agcaaaccga tagaataagg atgaacgggt
     4981 tgataaaaga catcgcgcgc gttgccatat tccatcgtgc gcccggcgta cattaccagc
     5041 actttgtcgc agatccccgc caccacgcca agatcgtggg taatcataat gatggcggta
     5101 ttaaattccc gcttcagttc atttaatagc gtcatgatct gcgcctgtac ggtgacgtcc
     5161 agcgcagtag tgggttcatc cgcaatcagc agcttaggtc gacatagcaa tgccatcgca
     5221 atcataactc gctgacgcat gccaccagaa aattcgtgcg ggtacatttt catgcgttta
     5281 cgtgcttccg gcatttttac cgcatcgagc atccgcaccg actcttcaaa cgcctcagct
     5341 ttgctcatgt tcttatgcag catcagcact tccatcaact gctcaccgac gcgcatatag
     5401 ggattcaacg aagtcattgg gtcctgaaaa atcattgaga tttgttcagc gcgcagttta
     5461 ttgagttcac gctctggcaa attgaggatt tcacgcccat tgaaggttgc cgatccgcca
     5521 atacgtccgt tggcagccaa caggcccatc aacgcaaatg cagtttgcga tttacccgaa
     5581 ccagactcac ctacaatgcc cagcgtttct ccggcacgta gagaaaaatt caaatcattg
     5641 accgccgtga catcgccgtc cggggtacta aaggtgacac gcaaatcttt cacgttcagc
     5701 agtgcgtcag cctgttgttg tgcgagcggc acagttgcag tttcaattac gctcatggct
     5761 gcactcctta acgatctttc gggtcgagag catcacgcaa gccatcgccg ataaagttga
     5821 aacaaaacag cgtcaccacg aggaatcccg ctgggaacaa caataaccat ggagagactt
     5881 ccatcgagtt cgcgccatca ctcagcaatg caccccagct gcttaacggc tcttgcgtac
     5941 ccaaccccag gaagctaagg aaagattcaa agaggatcat gctgggcacc agaagtgatg
     6001 cgtagaccac caccacaccg agtacattcg gcacaatgtg gcgaataaca atgcccgatg
     6061 tcgatacacc gccaacttgt gccgcctcaa taaactcttt gcgcttcaga ctcagggttt
     6121 gtccacgcac aatacgagcc atatccagcc aggaaaccat gccaatcgcc acgaaaatca
     6181 gcaggatgtt ttgaccgaaa aaggtcacaa gcaaaatgac gaagaacatg aatgggaagg
     6241 agttgaggat ttccagcaga cgcatcatta ccgaatccac tttaccgccc agataaccgg
     6301 aaagcgaacc gtaaagtgtc cccacgacca ctgccaccag tgccgcagca acaccgacca
     6361 tgagtgagat acgcccgcca atcgcaacgc gcacaagcag gtcgcgaccg gatgagtcag
     6421 taccaaagta gtgaccggac tccatatccg gggcgctgga catcatcgcc cagtcagtat
     6481 cgtcataggc aaactgcgaa agcatcggtg ccaggattac aaataacgcg atcagcacca
     6541 gcactatcag actggcaacc gccgcacggt tatgcataaa acgtcgacgt gcgtcctgcc
     6601 acaagctgcg cccttcgacc tccagctttt cactgaaatt ttccagcgtc tcgctgtttt
     6661 tcttacttaa catcatcgcg agctccagta tcagtaacgg attttcgggt cgataaccgc
     6721 atatagcaca tcgacaatgg cattaaacaa aatggttaaa gcaccaacca ggatggtcag
     6781 gcttaacact aaggaatagt cacggttcaa tgcgccatta acgaacagtt gtccaatccc
     6841 cggcaaacca taaatggttt cgataaccat agaaccggta ataatgccga caaatgcggg
     6901 gcccatataa gagagcacgg gtaacagagc aggttttaat gcgtggcgta aaatgatccg
     6961 ccgcataggt aaccctttcg cccgggcagt acgaataaag ttggagtgta atacttcaat
     7021 catagagcct cgggtaatac gcgcaatact ggcgatataa gcgagtgaca acgccaccat
     7081 cggcaatatc atgaatttaa gcgccccacc attccagcca ccgcccggca gccaatgcaa
     7141 aatgatcgca aatatcatga ctaataatgg cgcaaccaca aaactgggga taacaacccc
     7201 ggtcattgcc agccccatca cggtatagtc ccatttggtg ttttgtttta atgcgacaat
     7261 aacgccagca ctaacaccca gtattaccgc aaggaaaaat gcggcggctc ccagtttggc
     7321 agaaacggga aaactggatg caaccaggtc attgaccgaa taatctttat atttaaacga
     7381 tggaccgaaa tcaccgtgcg ccagttgttt caggtagctg aaatactgtg tcatgatcgg
     7441 atcattaaga tgatatttcg cttcgatatt ggccatcact tccggcggta aagtacgttc
     7501 gccggtaaaa gggcttcccg gcgcgaggcg catcataaag aacgaaatag taataagaat
     7561 aaatagcgtc ggaatcgctt ccagacagcg acgtagaata aattttaaca ttgccctacc
     7621 ttctggcctg tgccttctgt aatgcgataa aaatcagaca ccgtggagca ggacactcct
     7681 gccccacgta ttgccattag tgcttcacaa tgtacatatt ccgggtgtag gtattatcca
     7741 gcggatcttt gccggtatag ccaccaaccc acggtttcac cagacgcgca ttcacgtagt
     7801 aataaacagg aacaatggcc gaatccttat ccagctgttg ttctgcttta gtgtacagag
     7861 ctgtgcgctg cgcctcgtca gtcactttca gcgtttccgc cataatgctg tcaaaggccg
     7921 ggctcttata atgcgcggta ttcatcgagc tgttcgacag catggtgttc aggaaggaag
     7981 ttggttcgtt gtagtctgca caccagcctg cacgggccac atcaaaagtg ccctggtgac
     8041 gggtgtcgag gaacgttttc cactcctggt taaccagttt gacgtttaca ccaatgtttt
     8101 tcttccacaa tgaagaggca gcaatcgcca gctttttatg cagatcagag gtgttataca
     8161 gcaggttgat ggtcaacggt ttgtctgcgg tataacccgc ttcagccagc agttttttcg
     8221 cttcttcgtt acgtttttcc tggctccagc caaaccattc tggctgagtc aattttgcgc
     8281 catcagtata cggtggagtg taaccatagg cgggcatgtc gccctgcgct ttcactttat
     8341 taacaatgat atcgcggtcc atacctagtt tcagtgcggt acgcacacgc acatcgttga
     8401 atggcggttt ctggttgtta atttcgtaat agtaagtgca caggtatgga tcaacgtgaa
     8461 cttcgtccgg gatctctttt ttcagcttct ggaacaattc gatcggcatg ttgttatacg
     8521 tcatgtcgat ttcaccacta cggtagcggt tgacatcggt aacttcagaa gcaataggca
     8581 aataggttac ctggttaata acggttttcg cgttgttcca gtaggtcggg ctgcgttcaa
     8641 gaacgattcg ttcgtttacg acccaatctt ttaaggtata ggcaccgttg gtgacgatat
     8701 taccaggctg ggtccatttt tcgccgaatt tctcgatagc ggcttttggc accggtgaag
     8761 ttgatgggtg aacaagtaat ttatagaagt acggaacggg ttcacttaag gtgacttcta
     8821 atgtgtgatc atcaatagct ttcacgccga gatcggtaat cggttttttc ccttcaagaa
     8881 tttcatcaat accggcgata tgcccatatt gcagataact ggcatacgga gaagcagtgt
     8941 tcggatcaac agaacgttgc cagctataca caaagtcttg tgctgtgact ggcgtgctat
     9001 cagaccattt cgcatcttta cgcaaatgga aggtccagac tttcgcgtct ttattatccc
     9061 aggattcagc gacgccaggt gctggatgac cgtcaagatc gctgaccagt aagccttcaa
     9121 acaggtctcg gctgatatta gactccggaa caccttcaat tttgtgcgga tctaatgact
     9181 gaacttctga accattgtta cgtaccagtg tttgtttttc cgccagtgtg acgcctgcgg
     9241 gtacatcagc tgccagcgcg acattccctg ccattagcgc agccagaacg ccagctgcta
     9301 ctaaacttct cttggtgatg ttggtcattg ttttttggac tccctcatta taattactgg
     9361 tattaaccag catgtgtaat cccggattgg gaccctgcac aggctcggag actttctgct
     9421 gtcaggttac tttatcgtta cgtattacta tcaccgactt tatttattct ggtgattcag
     9481 acgatcacct tatgtcgatt cgtactgtgt ctcccctgtc gagacacatt attctttcgc
     9541 gacgctcttt aattgttaat tattctcatt accaataaag aatcgtctgg cggttgcccg
     9601 gaaagtacca aatgcggttc ttactcgcca atacattttg caaaattgtt aagcaattct
     9661 cttttattga ccgtatcaga atctatagct tgcatccatg ccttaacaaa catctttcaa
     9721 ttcaatcaaa tagagattgg ctaagcatgt ctatcttttc tgattacagt tccagtagtg
     9781 aaatgcacaa taatttaaca attgattatt atttagcact ttcctctacc aaaggcagtg
     9841 gaattactaa tatcatttca attattcttc agcaagccca ggattatgat gtggcgaaaa
     9901 taacatgatg gtgaggagaa gcagttaagg aaatccttat aactaactgt aatttgagaa
     9961 gaatattttt acctattcct tttgcttttg ataactgcgc gggaacaaag taaaaataat
    10021 gacgaagtaa acggagcatg gcagctccgt ttcatttgaa aggaattaat taagcaggcc
    10081 ggggaaaatc cccttaatac cagtaacgat aaattcaatc cccaatgcca tcagcaatag
    10141 ccccataata cgcgtaatca cgttgatgcc ggtctggcgt aaaacccgta ccagccacgg
    10201 tgccatgcgg aacaatcccc agcaacataa agcgaacaat gcaatagcca caaagaaacc
    10261 aaacagatag ctaatgctgt gataacgcgt accccagacg atggtagaac tgatcgcccc
    10321 tggccccgcc atcaacggca acgccaatgg caccacacca atgctttcac gtaccgcggt
    10381 ttctgatttt tcttgcttgt tctgtttatc ctcgccaagc ttgccgctga tcatcgacat
    10441 cgctatcgtc accaccagga tgccccctgc gatacggaac gaatcaattg atataccaaa
    10501 aagttgcaga atcgtgtcgc cgagaaaaag cgagatccac aagataatgg ccacagacag
    10561 gttggctgta aggttagttt tgtttcgcgc tgccgctgtc tgataactgg tcatgctgat
    10621 aaagacggga ataatcccta ccgggttgac cagcgcaaat aacccgatga aaaatttgaa
    10681 gtaaacggga aaatccaaaa aggtctgaat cacggttagc tccgaagcaa aagccggata
    10741 atgttagcca taaattaagg ttgaaaagac gcgctgacaa tacgcctttt gacagcattt
    10801 ttcacctcct aactacttaa aattgctatc attcgttatt gttatctagt tgtgcaaaac
    10861 atgctaatgt agccaccaaa tcatactaca atttattaac tgttagctat aatggcgaaa
    10921 agcgatgctg aaaggtgtca gcttagcaaa aatttgattt ggatcacgta atcagtaccc
    10981 agaagtgagt aatcttgctt acgccaccta gaagtgacgc attagagata ataactctaa
    11041 tgtttaaact cttttagtaa atcacagtga gtgtgagcgc gagtaagctt ttgattttca
    11101 taggttaagc aaatcatcac cgcactgact atactctcgt attcgagcag atgatttact
    11161 aaaaaagttt aacattatca ggagagcatt atggctgtta ctaatgtcgc tgaacttaac
    11221 gcactcgtag agcgtgtaaa aaaagcccag cgtgaatatg ccagtttcac tcaagagcaa
    11281 gtagacaaaa tcttccgcgc cgccgctctg gctgctgcag atgctcgaat cccactcgcg
    11341 aaaatggccg ttgccgaatc cggcatgggt atcgtcgaag ataaagtgat caaaaaccac
    11401 tttgcttctg aatatatcta caacgcctat aaagatgaaa aaacctgtgg tgttctgtct
    11461 gaagacgaca cttttggtac catcactatc gctgaaccaa tcggtattat ttgcggtatc
    11521 gttccgacca ctaacccgac ttcaactgct atcttcaaat cgctgatcag tctgaagacc
    11581 cgtaacgcca ttatcttctc cccgcacccg cgtgcaaaag atgccaccaa caaagcggct
    11641 gatatcgttc tgcaggctgc tatcgctgcc ggtgctccga aagatctgat cggctggatc
    11701 gatcaacctt ctgttgaact gtctaacgca ctgatgcacc acccagacat caacctgatc
    11761 ctcgcgactg gtggtccggg catggttaaa gccgcataca gctccggtaa accagctatc
    11821 ggtgtaggcg cgggcaacac tccagttgtt atcgatgaaa ctgctgatat caaacgtgca
    11881 gttgcatctg tactgatgtc caaaaccttc gacaacggcg taatctgtgc ttctgaacag
    11941 tctgttgttg ttgttgactc tgtttatgac gctgtacgtg aacgttttgc aacccacggc
    12001 ggctatctgt tgcagggtaa agagctgaaa gctgttcagg atgttatcct gaaaaacggt
    12061 gcgctgaacg cggctatcgt tggtcagcca gcctataaaa ttgctgaact ggcaggcttc
    12121 tctgtaccag aaaacaccaa gattctgatc ggtgaagtga ccgttgttga tgaaagcgaa
    12181 ccgttcgcac atgaaaaact gtccccgact ctggcaatgt accgcgctaa agatttcgaa
    12241 gacgcggtag aaaaagcaga gaaactggtt gctatgggcg gtatcggtca tacctcttgc
    12301 ctgtacactg accaggataa ccaaccggct cgcgtttctt acttcggtca gaaaatgaaa
    12361 acggctcgta tcctgattaa caccccagcg tctcagggtg gtatcggtga cctgtataac
    12421 ttcaaactcg caccttccct gactctgggt tgtggttcct gggggggtaa ctccatctct
    12481 gaaaacgttg gtccgaaaca cctgatcaac aagaaaaccg ttgctaagcg agctgaaaac
    12541 atgttgtggc acaaacttcc gaaatctatc tacttccgcc gtggctccct gccaatcgcg
    12601 ctggatgaag tgattactga tggccacaaa cgtgcgctca tcgtgactga ccgcttcctg
    12661 ttcaacaatg gttatgctga tcagatcact tccgtactga aagcagcagg cgttgaaact
    12721 gaagtcttct tcgaagtaga agcggacccg accctgagca tcgttcgtaa aggtgcagaa
    12781 ctggcaaact ccttcaaacc agacgtgatt atcgcgctgg gtggtggttc cccgatggac
    12841 gccgcgaaga ttatgtgggt tatgtacgaa catccggaaa ctcacttcga agagctggcg
    12901 ctgcgcttta tggatatccg taaacgtatc tacaagttcc cgaaaatggg cgtgaaagcg
    12961 aaaatgatcg ctgtcaccac cacttctggt acaggttctg aagtcactcc gtttgcggtt
    13021 gtaactgacg acgctactgg tcagaaatat ccgctggcag actatgcgct gactccggat
    13081 atggcgattg tcgacgccaa cctggttatg gacatgccga agtccctgtg tgctttcggt
    13141 ggtctggacg cagtaactca cgccatggaa gcttatgttt ctgtactggc atctgagttc
    13201 tctgatggtc aggctctgca ggcactgaaa ctgctgaaag aatatctgcc agcgtcctac
    13261 cacgaagggt ctaaaaatcc ggtagcgcgt gaacgtgttc acagtgcagc aactatcgcg
    13321 ggtatcgcat ttgcgaacgc cttcctgggt gtatgtcact caatggcgca caaactgggt
    13381 tcccagttcc atattccgca cggtctggca aacgccctgc tgatttgtaa cgttattcgc
    13441 tacaatgcga acgacaaccc gaccaagcag actgcattca gccagtatga ccgtccgcag
    13501 gctcgccgtc gttatgctga aattgccgac cacttgggtc tgagcgcacc gggcgaccgt
    13561 actgctgcta agatcgagaa actgctggca tggctggaaa cgctgaaagc tgaactgggt
    13621 attccgaaat ctatccgtga agctggcgtt caggaagcag acttcctggc gaacgtggat
    13681 aaactgtctg aagatgcatt cgatgaccag tgcaccggcg ctaacccgcg ttacccgctg
    13741 atctccgagc tgaaacagat cctgctggat acctactacg gtcgtgatta tgtagaaggt
    13801 gaaactgcag cgaaaaaaga agccgctccg gctaaagctg agaaaaaagc gaaaaaatcc
    13861 gcttaatcag tagcgctgtc tggcaatata aacggcccct tc
//
LOCUS       DAZPDU010000114        13853 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZPDU010000114 DAZPDU010000000
VERSION     DAZPDU010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13853
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.115"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(1..736)
                     /gene="dsbG"
                     /locus_tag="ACHURS_002988"
                     /pseudo
     CDS             complement(1..736)
                     /gene="dsbG"
                     /locus_tag="ACHURS_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
     gene            1108..1671
                     /gene="ahpC"
                     /locus_tag="ACHURS_002989"
     CDS             1108..1671
                     /gene="ahpC"
                     /locus_tag="ACHURS_002989"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFR1238659.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            1842..3407
                     /gene="ahpF"
                     /locus_tag="ACHURS_002990"
     CDS             1842..3407
                     /gene="ahpF"
                     /locus_tag="ACHURS_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFR1238660.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(3528..3956)
                     /gene="uspG"
                     /locus_tag="ACHURS_002991"
     CDS             complement(3528..3956)
                     /gene="uspG"
                     /locus_tag="ACHURS_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFR1238661.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVIR"
     gene            4177..5415
                     /locus_tag="ACHURS_002992"
     CDS             4177..5415
                     /locus_tag="ACHURS_002992"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HFR1238662.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDILGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            complement(5419..5508)
                     /gene="yldA"
                     /locus_tag="ACHURS_002993"
     CDS             complement(5419..5508)
                     /gene="yldA"
                     /locus_tag="ACHURS_002993"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFR1238663.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(5646..6056)
                     /gene="rnk"
                     /locus_tag="ACHURS_002994"
     CDS             complement(5646..6056)
                     /gene="rnk"
                     /locus_tag="ACHURS_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFR1238664.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(6286..7092)
                     /gene="rna"
                     /locus_tag="ACHURS_002995"
     CDS             complement(6286..7092)
                     /gene="rna"
                     /locus_tag="ACHURS_002995"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFR1238665.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKVGY"
     gene            complement(7206..8669)
                     /gene="citT"
                     /locus_tag="ACHURS_002996"
     CDS             complement(7206..8669)
                     /gene="citT"
                     /locus_tag="ACHURS_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFR1238666.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLSVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            complement(8720..9598)
                     /gene="citG"
                     /locus_tag="ACHURS_002997"
     CDS             complement(8720..9598)
                     /gene="citG"
                     /locus_tag="ACHURS_002997"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFR1238667.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEIAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            complement(9573..10124)
                     /gene="citX"
                     /locus_tag="ACHURS_002998"
     CDS             complement(9573..10124)
                     /gene="citX"
                     /locus_tag="ACHURS_002998"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFR1238668.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            complement(10128..11660)
                     /gene="citF"
                     /locus_tag="ACHURS_002999"
     CDS             complement(10128..11660)
                     /gene="citF"
                     /locus_tag="ACHURS_002999"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFR1238669.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANQEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            complement(11671..12579)
                     /gene="citE"
                     /locus_tag="ACHURS_003000"
     CDS             complement(11671..12579)
                     /gene="citE"
                     /locus_tag="ACHURS_003000"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFR1238670.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            complement(12576..12872)
                     /gene="citD"
                     /locus_tag="ACHURS_003001"
     CDS             complement(12576..12872)
                     /gene="citD"
                     /locus_tag="ACHURS_003001"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFR1238671.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            complement(12887..>13853)
                     /gene="citC"
                     /locus_tag="ACHURS_003002"
     CDS             complement(12887..>13853)
                     /gene="citC"
                     /locus_tag="ACHURS_003002"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFR1238672.1"
                     /translation="VDTTVEVFITVTRDEKLITCGGIAGNIIKCVAISESVRGEGLAL
                     TLATELINLAYERHSTHLFIYTKTEYEALFRQCGFSTLTSVPGVMVLMENSATRLKRY
                     AESLKKFRHPGNKIGCIVMNANPFTNGHRYLIQQAAAQCDWLHLFLVKEDSSRFPYED
                     RLDLVLKGTADIPRLTVHRGSEYIISRATFPCYFIKEQSVINHCYTEIDLKIFRQYLA
                     PALGVTHRFVGTEPFCRVTAQYNQDMRYWLETPTISAPPIELVEIERLRYQEMPISAS
                     RVRQLLAKNDLTAIAPLVPAVTLHYLQNLLEHSRQDAAARQKTPA"
BASE COUNT         3399 a         3507 c         3689 g         3258 t
ORIGIN      
        1 aatgatatta agcgttttct gatcgggcaa ccccacggcc tgttgcagcg tattttcctt
       61 actcatgtaa tagatagccg gcgtgacatt tgcccccaga tcgtccatca gtttctcatt
      121 gtcacttaac actttcattt gctctgtact tacgtttgca ggcacgttta gcttaagctt
      181 gccaccagag gcttcatatt gttgccaggt ttttgcggga tctttggagg caagaattgc
      241 cgctgctgtc gccgggcttt ctggcttgat aaccccaacc aacaatgttc ttaattgcac
      301 tttgccagaa tctacccacg ggcgcgcctg ctgccagaac tgtttacaat atgggcagaa
      361 cggatcggcg aagacgtaga caatgaccgg cgcatctttt ttaccgtcga ggatccagtg
      421 ggattgttcc atccgttgcc acatttcgcg tccggctggt gcgtaaattt ctttttcgat
      481 aagtgtgtta ctcaggtttt cacctttctc gttgtacatg taaccagaga tagcgtgctt
      541 accatcggga gtcaggtagg tagatggtga cgcccatatc ctgatacttt ccgagataac
      601 ctttcattcc tccgggggca tcgaacgttt tgatgattgt aatgccctgt ttttcaatcg
      661 cttttactgg ggaaggaagt tcctctgcga aggcgattgc aggaagcaga gccagtaaaa
      721 gtatcttttt taacattaat ttgtcctttt cagtcagtgc aaaagtcgag taaaaggcat
      781 aacctatcac tgtcataggt aagagcttag atcaggtgat tgccctttgt ttatgagggt
      841 gttgtaatcc atgtcgttgt tgcatttgta agggcaacac ctcagcctgc aggcaggcgc
      901 tgaagatacc aaagggtagt tcagattaca cggtcacctg gaaagggggc cattttactt
      961 tttatcgccg ctggcggtgc aaagttcaca aagttgtctt acgaaggttg taaggtaaaa
     1021 cttatcgatt tgataatgga aacgcattag ccgaatcggc aaaaattggt taccttacat
     1081 ctcatcgaaa acacggagga agtatagatg tccttgatta acaccaaaat taaacctttt
     1141 aaaaaccagg cattcaaaaa cggcgaattc atcgaaatca ccgaaaaaga taccgaaggc
     1201 cgctggagcg tcttcttctt ctacccggct gactttactt tcgtatgccc gaccgaactg
     1261 ggtgacgttg ctgaccacta cgaagaactg cagaaactgg gcgtagacgt atacgcagta
     1321 tctaccgata ctcacttcac ccacaaagca tggcacagca gctctgaaac catcgctaaa
     1381 atcaaatatg cgatgatcgg cgacccgact ggcgccctga cccgtaactt cgacaacatg
     1441 cgtgaagatg aaggtctggc tgaccgtgcg accttcgttg ttgacccgca gggtatcatc
     1501 caggcaatcg aagttaccgc tgaaggcatt ggccgtgacg cgtctgatct gctgcgtaaa
     1561 atcaaagcag ctcagtacgt agcttctcac ccaggtgaag tttgcccggc taaatggaaa
     1621 gaaggtgaag caactctggc tccgtctctg gacctggttg gtaaaatcta aatttccttc
     1681 gtctttcgcg ctaccaactc cggcgcgaaa tacttcggaa attcacctaa ttcttcgggt
     1741 gctgcggcac ccgattttct tccccgcacc atgatgcaag ctgcatccag gtagccgtag
     1801 aggccgcttg catgatgatg tttaaagccc aggagataaa catgctcgac acaaatatga
     1861 aaactcaact caaggcttac cttgagaaat tgaccaagcc tgttgagtta attgccacgc
     1921 tggatgacag cgctaaatcg gcagaaatca aggaactgtt ggctgaaatc gcagaactgt
     1981 cagacaaagt cacctttaaa gaagataaca gcttgccggt gcgtaagccg tctttcctga
     2041 tcaccaaccc aggttccaac caggggccac gttttgcagg ctccccgctg ggccacgagt
     2101 tcacctcgct ggtactggcg ttgctgtgga ccggtggtca tccgtcgaaa gaagcgcagt
     2161 ctctgctgga gcagattcgc catattgacg gtgattttga attcgaaacc tattactcgc
     2221 tctcttgcca taactgcccg gacgtggtgc aggcgctgaa cctgatgagc gtactgaacc
     2281 cgcgcatcaa gcacactgca attgacggcg gcaccttcca gaacgaaatc accgatcgca
     2341 acgtgatggg cgttccggca gtgttcgtaa acgggaaaga gtttggtcag ggccgcatga
     2401 cgttgactga aatcgttgcc aaaattgata cgggcgcgga aaaacgtgcg gcagaagagc
     2461 tgaacaagcg tgatgcttat gacgtattaa tcgtcggttc cggcccggcg ggtgcagcgg
     2521 cagcaattta ctccgcacgt aaaggcatcc gtaccggtct gatgggcgaa cgttttggtg
     2581 gtcagatcct cgataccgtt gatatcgaaa actacatttc tgtaccgaag actgaagggc
     2641 agaagctggc aggcgcactg aaagttcacg ttgatgaata cgacgttgat gtgatcgaca
     2701 gccagagcgc cagcaaactg atcccagcag cagttgaagg tggtctgcat cagattgaaa
     2761 cagcttctgg cgcggtactg aaagcacgca gcattatcgt ggcgaccggt gcaaaatggc
     2821 gcaacatgaa cgttccgggc gaagatcagt atcgcaccaa aggcgtgacc tactgcccgc
     2881 actgcgacgg cccgctgttt aaaggtaaac gcgtagcggt tatcggcggc ggtaactccg
     2941 gcgtggaagc ggcaattgac ctggcgggta tcgttgagca cgtaacgctg ctggaatttg
     3001 cgccagaaat gaaagccgac caggttctgc aggacaaact gcgcagcctg aaaaacgtcg
     3061 acattattct gaatgcgcaa accacggaag tgaaaggcga cggcagcaaa gtcgttggtc
     3121 tggaatatcg agatcgtgtc agcggcgata ttcacaacat cgaactggcc ggtattttcg
     3181 tgcagattgg tctgctgccg aacaccaact ggctcgaagg cgcagtcgaa cgtaaccgca
     3241 tgggtgagat tatcattgat gcgaaatgcg aaaccaacgt caaaggcgta ttcgcagcgg
     3301 gtgactgtac gacggttccg tacaagcaga tcatcatcgc cactggcgaa ggtgccaaag
     3361 cctctttgag tgcttttgac tacctgattc gcaccaaaac tgcataagaa gaagtaagat
     3421 tcacctgcaa ttgcttagcc gccggggtca aacctggcgg cttttttatg gcattaaaaa
     3481 gccctgccgg gatgttccag gcagggcggc ataaatactt ttatctgtta gcgaatgacc
     3541 agtacaggca gattggcgtg gcggatcacg ctcgaggcgt tagaacctaa cagatgggtc
     3601 gaaatcgatg ggttgcgaga accgataact acaacatcag cccccagttc ttctgccaac
     3661 tcattgactt catcccgcac gctaccaaaa cggacatgtt gtttaatgcg ggaaggatcg
     3721 atggtgaagt ggctgaccat cgtttgcaga cgttcttctg cttcatgttg cagatgctct
     3781 tcaaaacgac gcacatcagc ggcaaaacgg tgcaggctca ggctggctga cccgggtagt
     3841 acgtgaagta gatgaataac tccgtcatcc tgggcgagga attcagcgtg gcgaacagct
     3901 ttgtcgctca attccatttc aaatacatca actggcataa tgattgtctt atacataacc
     3961 ctttctccct gttaatcatg aacaaatcat tcgccatgat tataatattt atccctgata
     4021 tttgtctggt tctttttcct tacgaacggt ttctgtgatt aatatattct cactgaacac
     4081 caggaattct cccaaaacct gcggtaccgc ccgttttccc gctgtgatag ctacccttaa
     4141 agactgactc ttttttgaac tgtctctgga ggttgcatga aagcattgac ttatcacggc
     4201 ccacatcacg ttcaggtaga aaatgttccc gatccgggca ttgaacaggc tgatgatatt
     4261 attctgcgta ttacggcaac ggcgatctgt ggctctgacc tccatcttta tcgaggcaaa
     4321 ataccccagg ttaaacatgg cgatattttg ggtcatgaat ttatggggga agtcgttgaa
     4381 accggaaagg acgtaaaaaa tttgcaaaaa ggcgaccggg tggtaattcc gtttgtcatt
     4441 gcttgtggcg actgtttttt ctgtcgaatg cagcaatatg ccgcctgcga aaataccaat
     4501 gcgggtaaag gcgctgcgct caataaaaaa cagataccag ctcccgcggc attgtttggt
     4561 tatagtcacc tgtatggtgg cgttcctggt ggacaggcgg aatatgtccg cgtccctaaa
     4621 gggaatgtgg ggccgtttaa agtaccgcct ttgctttcag atgataaagc ccttttcctt
     4681 tctgatattc tgccaacggc atggcaggca gcaaaaaatg cgcagatcca acaaggttca
     4741 agcgttgcag tctatggtgc cggtccagtg ggattgttga caatcgcctg tgcacggttg
     4801 ctcggtgcgg aacagatttt tgttgttgat catcatccct accgcttgcg tttcgccgct
     4861 gaccgctacg gcgcgatccc gattaatttc gatgaagata gcgatccggc acagtcaatt
     4921 attgaacaaa cggcaggtca ccggggcgtg gatgcagtaa tagacgccgt cggttttgaa
     4981 gcgaaaggca gcaccacgga aacggtgctg actaacctga aactggaggg cagcagcggt
     5041 aaagcgttgc gccagtgtat tgcggcggtc aggcgtggcg gcattgttag cgtaccgggc
     5101 gtctacgctg gatttattca cggtttcctg tttggcgacg cctttgataa agggttgacg
     5161 tttaaaatgg gacagaccca cgttcacgca tggctgggtg aattattacc gttaattgag
     5221 aaaggattac tgaaaccaga agaaattgtt acccactata tgccgtttga agaggccgcc
     5281 cggggatatg agattttcga aaaacgtgaa gaggagtgcc gtaaggtgat tctggtaccc
     5341 ggtgcacaaa gcgcagaggc ggcgcagaag gcggtttcag gtctggtgaa tgcgatgccg
     5401 gggggaacaa tatgatcgtc aggagtggtt ttcgaggtaa aggacagcca tgacgataat
     5461 cgccgccata atcagaaatc ctatcaggat gtaaaatgct tctgccatgg ttattcccac
     5521 aaacgaaacg gaataatttt gcagcaaaag tgaacagtga gaaccaggaa aaattgctga
     5581 ttttgcgtaa agaggatgcg agtgcatcct ctgggcaaag cgagttatcg cttgtgcaga
     5641 tgggattaaa gcaggtagtc gccagcagct tctggctggt attcgagttc cagcacttca
     5701 aggtgggttg caacgccgcc cggaagttcc cagtgaatgg aatcgccaac gcgcagcccc
     5761 agcagtgcgg cacctaccgg agccataacg gaaagctgag tattgctatc ggtcattttt
     5821 gccggataca ccagcgtgcg cacacgcact tcgccatcgc taagattgcg gaatttaacc
     5881 cggctgttca ttgtcaccac gtcgtgtggc atctcttctg gcgaacacat ttgggcgcga
     5941 tccaactctg cgtttaacgc gtcggcgatt ggcaaaccag cataggcggg ttgctccagc
     6001 agaatatcga tgcgttcggc atccaggtcg ttaatgatga tagttggtct ggacattttt
     6061 actccatgtc gtcggtactg cgagtgtcgc agataaacat acccaaaaga aaaccctcac
     6121 cgtcaggcgg cgagggttta actcacatga tgatactgac tgttgctcac tctttgaagt
     6181 gatttgcgtc acattcaggg aattcctcaa tgcagcgcat tatgtataat tcttaatcgt
     6241 cacggtttat ggaagacgaa ttagcgtgtt ttgtaaatca gatgattaat aacccacttt
     6301 atcaatcaca aaggttttgc cacagttgcc tgggtgtggt tgtggcaaga atgagtttgc
     6361 agaaagcgga gcgttgatgg cgtcagcttt gatcgagatc tgaatttcag tcaaatacgc
     6421 agggttaccc tggcacgtta gcttaactgc tttcacgttc tcttttcccc agcttttggc
     6481 aaaggcggcg tcaaagtcac ggcggctcac tgttttaccg taattatccg caaggaattt
     6541 tccggcttcg ctctctttaa tttcttgatt caggcgtacc atcgtaccga agtatgcgtc
     6601 cggatcaaaa ccaaagcagg caccgtgttt ggcatattcg tagcgttcca ggcaggaacg
     6661 tccgccagct cctggcatga cttcacttag tttagcggcc gtttccagtg ataacccggt
     6721 ttccggcgat gaacacatcc ggctggcgtg cgcttctggt agattcggga ttgggcgagt
     6781 agcgcaaccg aagcgcatcc agcggcgttc atcaacacca cgggcagcaa ccgatttagg
     6841 caatcctggc cacagaccat gtacggtcag aaaatcagct ttgttggtcg tttcggtttg
     6901 taggcgacat tcatctcgtt cgttacgatt tcgatcgtgt tgactctggc aaaatccggt
     6961 ttgccaggag agggccagga catagcgatc aaaatcgcca tactgttttg cctgcaacgc
     7021 tacggcgttg gcagaagaga agggaagcag agaaaccgcg agcaacgcgg cgttacgcca
     7081 gaatgctttc ataatgggtg tggaactcat acatacactg aatactatct attaaatcat
     7141 aaaaaagccc gccaggtggg cttacctggc gggcgtgatg atttattcag cgtttggcga
     7201 acgtattagt tccacatggc gagaatcggc cagccaacca acagcaacat agagatgtaa
     7261 atcaccccga agattgcgcc aagacgccag taatcttttg atttcacata gccacagccg
     7321 taaataatca ccccaggacc ggttgcatac ggcgtcagac agcccatgat accgatagac
     7381 agcaccagca ggatacacag ttgttccatt ggtacgcccg gaataccttt accgacggcc
     7441 agaataaccg acagcatggt tgcggtgtgc gcagacaggc tggcaaacag gtagtgtgca
     7501 aagtagaaca ccagaaccag tacaatcacc gttgcgtttg gtgagaatcc ttccaggtgc
     7561 gtactcatgg taccggcgaa ccagtcaata aagccagaac gagtcaggcc gttagccatc
     7621 acaaccagag ttgccaggtt gaccagcgtg ttccatgcgc tgttatagcg ggtaatatct
     7681 ttccaaggca caacgtgcag ggccagcatt agcgaaactg ccagcagacc aactgcagta
     7741 gcattaatga cttcactgcc aaaaacccac aaacctaagc tgagcaatac aaggccaatc
     7801 agtgtccact ctctgcgtgt cagcgcaccc atggttttca gttcatcacc cgcccaggtt
     7861 gccacttctt cactgtgtgt gatttccggt ttgtacagca cgtaggaaag ccacggcgca
     7921 atgataagca agataacccc aaccggcagg aagcagagga accactgcaa ccagctaatc
     7981 tggataccgg caattttgct gacgaactcc agacccagca cgtttggtgc cgcaccggtg
     8041 acaaacatgg acgaactcag actggtacta atgaccatca tccacatcaa atagccgcca
     8101 atacgacgcg cggacggatc gttggggaat gatttaaaca gcggcggcag gtttttaatg
     8161 accggaaaaa ccgtaccccc ggtacgcgcg gtgttggacg gtgtaaacgg tgccagcaga
     8221 atgtcgataa tgacaatcgc ataacccaac gtcagcgtgc gtttgcccat gaatttcacc
     8281 aggaaaaggg caatgcgacg acctaacccg gaaacttcat accctaatgc aaaaataaat
     8341 gcgccaaata ccagccatac cgtggtgctg gaaaaaccag ccagacccca tttcagcgcc
     8401 tgtttttgcg cattaaacgc tgggtcagct aattctttgg catcaaagag caggtaatta
     8461 ctgccaataa cgcaaatagt aaccgcaata aaactgatcg ctgttgccgg aattggctcg
     8521 aggatcatgc cgacaatcat tgccacaaac acagcgaagt aatgccatgc ctgtggtggc
     8581 ataccgtcgg ggacagggat aagaaacatg acacccatca ccaccagtgg ggccaatagt
     8641 ttccatatat tatcttttgc taaagacata cgggttctcc gaaaattaat atttccaaat
     8701 ttatcaagtg cttaaataat taaatctgtg ctaaaaacca ggtaaggatc agcaggtcgg
     8761 cactgccgcc tggactgaga tttcgttcga tacactccct gtcgaactgc cggagataat
     8821 cgagatcggc gggggttcga atgccccctt tttgcaataa tgtttgcgcc tcgcgctgta
     8881 gccagcgcag gcccccctcg ccaccgcgcg atgcaacgtt ggtatcgccg ttgatcgcca
     8941 tcagtaggag caaggtatcg agcaatgcca gttcaggatc taacccctga tccagcagag
     9001 tgaggtaatg cggcaaggcg tgattgatca ccagtggata acccgcttcg gcttcaccgc
     9061 gtgcgccggt aaggccaagc tgctggtaca accgttgacc tgccgtcagt tgtgaattat
     9121 tggtacgcag ttctcgatcg gtcaggccac ggcagaaact tgccgccgta gaacaaacgg
     9181 ttgttggcgt taccggttgg ttgagttgaa gcaaacggcc aattgccgca catagtagcc
     9241 ctaaagaaaa aatgctgcct ttatgcgtgt ttacgcccgc agtggcgcgg aacatatcac
     9301 cttcgcaagc cataccaatt gggcgtaatc cgtggagtac cgcttctggt gctatttccg
     9361 cactacaggc accaaattca atgaaacggg gtagccagcc ctgaatcgcc agcgcgctgc
     9421 ggtggaaatc ttccagcgcc atatctttat gtgcaccgca gttaatccga tccacgaggc
     9481 ctggtttcgg tgacagattg acttcagtca gcatggcgcg ccagcccagc agggcgtact
     9541 catcgatcaa tgacgtcgca agctttgtgg ttttagttga cgttgcaggc atcgacatcg
     9601 ttcagcagtg cctccatgcg gttgagtaaa tcggtcagtt gatgggtttt tccacgcgcg
     9661 cagacggctg cgctttgctc gcacaacagg cagcggcgag gcggcagtga atagtcgcgg
     9721 cgggagagaa tttcgccttc gggcgtcagg acatcgatat cccataaccg cccgagagga
     9781 tgactatgtt caagctcaat ggtggcgagc ttgaggtcgc gagccggggc ggcaatgctc
     9841 aacatgccct ccggcccgct ggcggaaacc agtgcagcct gctcctgaat ttgccagccc
     9901 tgttttgcgg ctaaggcacg caaggctgtc acgccatgat taaaaattcg gcgtgtgacc
     9961 tcgctgtctt taatcggccc aggcgcaacc acggtaaagg agaccagtgg aacaggatgg
    10021 cgcttgagcc agacgtgttg ccgtgcttgc ctttcatccc ggctgacgag cagctcggga
    10081 attgacaccg catggtggct ggcgagttca ggaagcaggt gcatggctta ttccttcacc
    10141 tgatgcacaa catcgatcac cgagccatcg cggtaacgca caacggcaac gacgcggtcg
    10201 gtgaattcaa tcggctgtgg ttcaccggtc agcagacgcg cgcgttcgcg cagccactca
    10261 atggaaacca ctttaatgcc cgcttcctgc agacgttctg ccagttccgg acgtgccggg
    10321 ttaactgcga taccgtggtc tgtgaccaga atatcgacac tggagcctgg ggtgatgcag
    10381 gtcagcacgt tatccaccag agtcggaata cgaccgcgta ccagcggcgc gacgatgatg
    10441 gaaagcgcag aggcaatcgc ggtatcgcag tgaccaccgg aagcaccacg cagtacgccg
    10501 tcagagccgg tcagcacgtt aacgttgaac tgggtgtcaa tttccagcgc gctcagtacc
    10561 accacgtcga gacgatcaac cgatgcgcct ttcgaacccc agttagcgta ctggttggcg
    10621 ctgatttcga tgtgattggg gttacgggcc agcgattgcg cagcatggct gtcaaagctc
    10681 tgcacatcca gcagtttgcg gatcagacct ttttcgtgca gatcaaccat cgtcgcggta
    10741 ataccgccga gggcgaagtc ggcgcggata tcgcggctac gcattttgtc ttccaggaaa
    10801 cgggttaccg ccagcgatgc gccgccggtg ccggtttgca tggagaaacc ttctttgaag
    10861 tagccagagt tgacaatcac atccgcagcg ctacgggcaa taagcagttc gcgcgggtta
    10921 gtggtcatac gggtcgcgcc agcgccgatt tttgcagcat cgccaacgcg gtcaactttg
    10981 acgatcaaat caacctgatc ttgctcaatg cttgccggat tatgcggata aggcagcagt
    11041 tcttcggtaa gcatcacaac ctgttttgcg ttgtcggcat caaccattgc atagccgagg
    11101 gagccgcagc aggctttacc ggtgtaaccg ttggcattac cgaattcatc acaggacggg
    11161 acgccgagga aagccacgtc gatattcagt tcgccgctct gtaccagatg cacacgtccg
    11221 ccgtgagagt ggatttgcac cggttccgca agcagaccac gggaaatttc ttctgccagt
    11281 ggaccgcgca ggccggaggt ataaatgcgg gtaaccacgc cctggcgaat gtgttctacc
    11341 agtggcgcat ggcaatcact cagggagctg gacgccaggg tcaggttttt aaagcccatc
    11401 ttagcgatga cgtccatcac catattgacg gtcaggtcac cgccacggaa agcgtgatgg
    11461 aaggaaaccg tcatgccgtc ctgtaaacca gagcgacgaa tcgcttcttc ctggttggcg
    11521 cacagtttgc gatcgcgcgc tttttcagcc tggtaggttt gctttggtga gttctggaaa
    11581 gcggcaagat cgcattcagc gcgacgattc caggccgcta cccgttcttg tcgttgagat
    11641 tgttcaattt tctgcgtcat tttgattgcc ttattcttcg cggatgccgg aaagttctgc
    11701 acgggagagc accagacggg cacgatcgat aaccggaccg tccaccatct tgccgttcag
    11761 ggaaaccacg ccgaggcctt cgcgagcggc ggcttcagcg gcttctacga cgcggcgggc
    11821 gtgatccact tctttctggg tcggtgcgta gaggttgtgc aacagatcaa tctgacgcgg
    11881 gttgatcagc gatttgccgt caaagcccag ctgtttgatg tgggcggctt cttgcaggaa
    11941 tccggcttcg ttgttagcgt cggaatagac ggtatcgaac gcctgaatac ccgcagagcg
    12001 cgcggcctgc aaaatggaac agcgtgcgaa cagcagttca gttccttccg gggagcgttc
    12061 tgtacgcagg ttgcgcacat agtcttctgc accgagggcg ataccgatca aacgctcgga
    12121 agcgtgagcg atttccactg cgcgggtaat ccccagcgga gattcaatcg ccgccagcag
    12181 gccggtgctg ccgggttcac gaccacaggc tttttcgata cgcaggatct ctttttcgat
    12241 atccagaaca tcctgagcgg tatcggtttt cggcagacgc acaacgtccg caccaccgcg
    12301 aacgacggct tccaggtcgt tgacacccca ttcggaatcc agcgcgttga cacgcacaat
    12361 ggtttcaata tcgcgataca gcggatgttg cagcgcgtgg taaaccatgc ggcgggcggt
    12421 gtctttttca cgcaatgcta cggagtcttc gaggtcaaac atcagggcat cagccgggta
    12481 gatgaaggag ttgctgacca tcgcggcatt ggcaccaggc acaaacaaca tgctgcggcg
    12541 ggtgcgagtt ttacgttgtt gcagcgaagc ggaaatcatt ggcaatcctc ccatggcaga
    12601 gccgggatac cgctggcgcg tgccagcagg gcttccagtc gtgcacgtaa aatgcagtcc
    12661 agtgcgcctt tgtcatcgac attcagctgt acgccgcgca cgttgtagcg ggcgagaacg
    12721 tccagaatgg tggtgcgaat tgcatcgcca aactgtttct caacgctgct attgatttgc
    12781 aggtcgatat cctgcgtatc gagtggggcg atgcgtatca tcacatcccc agactcaagg
    12841 gtgcctgcaa cggcgggctg gtttattttc atttttcacc tgtttctcat gcgggggtct
    12901 tttgacgagc tgccgcgtcc tggcgggagt gctcaagcag gttctgcaaa taatgcagcg
    12961 tgactgcagg gaccagcggc gcgatagccg tgagatcgtt tttcgccagc agttgacgta
    13021 cccgggaagc ggatatcggc atctcctggt aacgcagccg ctcaatttca accagttcga
    13081 tgggcggtgc ggagatagtc ggcgtttcca gccagtagcg catatcctgg ttgtactggg
    13141 cggtaacgcg acaaaagggt tcagtaccga caaagcggtg agttacaccc agcgcgggag
    13201 cgaggtactg acggaaaatc ttcagatcaa tttcggtgta acaatggtta atgacgctct
    13261 gttctttaat gaagtagcaa gggaacgtag cgcgggagat gatgtattcg gagccgcgat
    13321 gcacagtcag gcgtggaata tcggcggtgc cttttaacac cagatccaac cggtcttcat
    13381 aggggaagcg tgaagaatct tctttgacta aaaacagatg caaccagtcg cactgtgccg
    13441 cagcttgttg aatcagataa cggtgaccat tcgtgaaggg attggcgttc atcacaatgc
    13501 agccaatctt gttccctgga tgacgaaatt ttttcagcga ttcggcatag cgtttcagcc
    13561 gcgtggcgct gttttccatc agcaccatca cgccgggtac gctggtcagc gtggaaaaac
    13621 cgcactggcg gaacagcgcc tcgtattcgg ttttggtata aataaacaga tgcgtgctgt
    13681 gccgctcata ggcgaggttt atcaattcag tggctaatgt cagcgccagt ccttcaccgc
    13741 gaacggattc actgatagca acgcatttaa taatatttcc ggcaattcca ccgcacgtga
    13801 taagcttttc atcgcgggtt acggtaataa atacttcgac tgtggtgtca acg
//
LOCUS       DAZPDU010000115        13748 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZPDU010000115 DAZPDU010000000
VERSION     DAZPDU010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13748
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.116"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..131)
                     /locus_tag="ACHURS_003003"
     CDS             complement(<1..131)
                     /locus_tag="ACHURS_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1238673.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPE"
     gene            240..734
                     /gene="napF"
                     /locus_tag="ACHURS_003004"
     CDS             240..734
                     /gene="napF"
                     /locus_tag="ACHURS_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFR1238674.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            724..987
                     /gene="napD"
                     /locus_tag="ACHURS_003005"
     CDS             724..987
                     /gene="napD"
                     /locus_tag="ACHURS_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFR1238675.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            984..3470
                     /gene="napA"
                     /locus_tag="ACHURS_003006"
     CDS             984..3470
                     /gene="napA"
                     /locus_tag="ACHURS_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFR1238676.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            3477..4172
                     /gene="napG"
                     /locus_tag="ACHURS_003007"
     CDS             3477..4172
                     /gene="napG"
                     /locus_tag="ACHURS_003007"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFR1238677.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            4159..5022
                     /gene="napH"
                     /locus_tag="ACHURS_003008"
     CDS             4159..5022
                     /gene="napH"
                     /locus_tag="ACHURS_003008"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFR1238678.1"
                     /translation="MANRKRDAGREALVKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSCSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            5019..5468
                     /gene="napB"
                     /locus_tag="ACHURS_003009"
     CDS             5019..5468
                     /gene="napB"
                     /locus_tag="ACHURS_003009"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFR1238679.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            5478..6080
                     /gene="napC"
                     /locus_tag="ACHURS_003010"
     CDS             5478..6080
                     /gene="napC"
                     /locus_tag="ACHURS_003010"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFR1238680.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            6093..6716
                     /gene="ccmA"
                     /locus_tag="ACHURS_003011"
     CDS             6093..6716
                     /gene="ccmA"
                     /locus_tag="ACHURS_003011"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFR1238681.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFDDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            6713..7375
                     /gene="ccmB"
                     /locus_tag="ACHURS_003012"
     CDS             6713..7375
                     /gene="ccmB"
                     /locus_tag="ACHURS_003012"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFR1238682.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            7417..8154
                     /gene="ccmC"
                     /locus_tag="ACHURS_003013"
     CDS             7417..8154
                     /gene="ccmC"
                     /locus_tag="ACHURS_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFR1238683.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            8151..8360
                     /gene="ccmD"
                     /locus_tag="ACHURS_003014"
     CDS             8151..8360
                     /gene="ccmD"
                     /locus_tag="ACHURS_003014"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFR1238684.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            8357..8836
                     /gene="ccmE"
                     /locus_tag="ACHURS_003015"
     CDS             8357..8836
                     /gene="ccmE"
                     /locus_tag="ACHURS_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFR1238685.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMLGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            8833..10776
                     /gene="ccmF"
                     /locus_tag="ACHURS_003016"
     CDS             8833..10776
                     /gene="ccmF"
                     /locus_tag="ACHURS_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFR1238686.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMIASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASYSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            10773..11330
                     /gene="dsbE"
                     /locus_tag="ACHURS_003017"
     CDS             10773..11330
                     /gene="dsbE"
                     /locus_tag="ACHURS_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFR1238687.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            11327..12379
                     /gene="ccmH"
                     /locus_tag="ACHURS_003018"
     CDS             11327..12379
                     /gene="ccmH"
                     /locus_tag="ACHURS_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFR1238688.1"
                     /translation="MRFLLGMLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVAYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIDGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(12414..13061)
                     /gene="narP"
                     /locus_tag="ACHURS_003019"
     CDS             complement(12414..13061)
                     /gene="narP"
                     /locus_tag="ACHURS_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFR1238689.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            13381..>13748
                     /locus_tag="ACHURS_003020"
     CDS             13381..>13748
                     /locus_tag="ACHURS_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR1238690.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVGLE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAVNQTTLNNGVLQVYG"
BASE COUNT         2938 a         3462 c         4037 g         3311 t
ORIGIN      
        1 atctctggtg aattaatcgc tatataaata tgtttataac catttgaaat gtgagcaaaa
       61 gcccgttttt gccacgcttc ccgccactct tttgatcctg ctacaggttt taccccgatc
      121 ggggtatgca tctttgacac atcctttaat atcttagcgg ctataaaaat ggcttattaa
      181 ttatgcggtt tatttggtcg ctctcaattt tcagagcgcg ttaatgatgg aaggtcaatg
      241 tgaagattga tgcatcccgt cggggcatac tcactggtcg ctggcgcaaa gccagtaacg
      301 gtatccgtcc gccctggtcc ggtgatgaat ctcattttct gacccattgc acccgttgcg
      361 acgcctgtat taatgcttgt gaaaacaaca ttctgcaacg cggcgcgggc ggctatccga
      421 gcgttaattt caaaaataac gagtgcagct tctgttacgc ctgcgcgcag gcttgccccg
      481 aatcgctatt ttctccgcgc cacaccaggg cctgggattt gcagttcacc attggggacg
      541 cgtgcctggc gtatcagtca gttgaatgcc gccgctgtca ggatagctgt gagccaatgg
      601 caattatctt tcgcccgacg ctctccggga tctaccagcc gcaacttaat agccaacttt
      661 gcaacggatg tggagcatgt gcagccagct gtccggtatc agccataact gcggagtatc
      721 tccatgcaca ctaactggca agtttgcagc ctggtcgtgc aggccaaaag cgaacgaatt
      781 tcagacatca gcacccaact gaacgccttt cccggctgtg aagttgctgt cagcgacgcg
      841 ccgagcggtc agttgattgt ggtggtggaa gcagaagaca gcgaaacgct gatccaaacc
      901 attgagtcag tacgcaacgt agagggcgtg ctggcggtgt cgctggttta tcaccagcag
      961 gaagagcaag gtgaggaaac accatgaaac tcagtcgtcg tagctttatg aaagctaacg
     1021 ccgttgcggc cgctgcggcg gctgccggtc tcagcgtgcc gggcgttgcc cgcgccgttg
     1081 ttggtcagca ggaagccatt aaatgggata aagcgccgtg ccgtttctgc ggtactggtt
     1141 gcggcgttct ggtcggaacg cagcaggggc gtgtggtggc ctgtcagggc gacccggacg
     1201 caccggttaa ccgtggcctg aactgcatta agggctattt cctgcccaag atcatgtacg
     1261 gtaaagaccg tttgacgcag ccgctgctgc gtatgaaaaa cggtaaatat gacaaagaag
     1321 gcgaatttac cccaatcacc tgggatcagg ccttcgatgt gatggaagag aaattcaaaa
     1381 ccgccctgaa agaaaaaggg ccggaatcga tcggtatgtt cggttctggt cagtggacta
     1441 tctgggaagg ttatgccgcg tccaagctgt ttaaagcggg cttccgttcg aacaacatcg
     1501 acccgaacgc gcgtcactgt atggcgtcgg cagtagttgg ctttatgcgt acctttggta
     1561 tggatgagcc gatgggctgc tatgacgaca tcgagcaggc tgacgcgttt gtgctgtggg
     1621 gcgcaaacat ggcggagatg cacccgatcc tctggtcgcg catcactaac cgtcgtctct
     1681 ctaatcagaa cgtcaccgtg gcggtgcttt ctacctacca gcatcgtagc ttcgagctgg
     1741 cggataacgg catcatcttt acgccacagt ctgacctggt gatcctgaac tacatcgcca
     1801 actatatcat tcaaaacaat gcgataaatc aggacttctt cagcaaacac gttaacctgc
     1861 gcaaaggggc gacggacatc ggctacggtt tacgtccgac ccatccgctg gaaaaagcag
     1921 cgaagaatcc aggttctgac gcctccgaac cgatgagctt tgaagattac aaagccttcg
     1981 ttgccgagta tacgctggaa aaaaccgctg aaatgaccgg cgtgccgaaa gaccagttag
     2041 aacaactggc gcagctgtat gccgatccga acaagaaagt catctcctac tggacgatgg
     2101 gcttcaacca gcatactcgt ggcgtgtggg ctaacaacct ggtctacaac ctgcacctgc
     2161 tgaccggcaa aatttcccag ccgggttgcg gtccgttctc cctgaccggg caaccttctg
     2221 cgtgtggtac tgcgcgtgaa gtgggcacct ttgctcaccg tctgcctgcg gacatggtgg
     2281 tgaccaacga gaaacaccgt gatatctgcg agaagaagtg gaatatcccg agcggcacca
     2341 ttccggcgaa aatcggtctg cacgcggtgg cacaagaccg tgcgctgaaa gacggcaaac
     2401 tgaatgttta ctggaccatg tgtaccaaca acatgcaggc cgggccgaac atcaatgaag
     2461 agcgtatgcc gggctggcgc gatccgcgca acttcatcat cgtctccgat ccgtatccga
     2521 cagtcagtgc gctggccgcc gacttgatcc tgccgaccgc aatgtgggta gagaaagagg
     2581 gtgcttacgg taacgccgaa cgccgtactc agttctggcg tcagcaggta caggcaccgg
     2641 gcgaagcgaa atcggatctc tggcagttag tccagttctc ccgccgcttc aaaactgaag
     2701 aagtgtggcc ggaagagctg ctagcgaaga aaccggaact gcgtggcaaa acgctgtacg
     2761 aagtgcttta tgccacaccg gaagtgagca aattcccggt atccgaactg gcggaagatc
     2821 agctgaacga tgaatcccgc gagctgggct tctatctgca aaaagggctg ttcgaagagt
     2881 acgcatggtt tggtcgcggt cacggtcacg atctcgcgcc gttcgatgac taccacaaag
     2941 cgcgcggtct gcgctggccg gtggtgaacg gtaaagaaac gcagtggcgt tacagcgaag
     3001 gtaacgaccc gtacgtgaaa gcgggcgaag gctataagtt ctacggtaaa ccggatggta
     3061 aagcggtgat cttcgcgctg ccgttcgaac cggcggcgga agcaccggat gaagagtacg
     3121 acctgtggct ctctaccgga cgcgttctgg agcactggca cactggcagt atgactcgcc
     3181 gtgtgccgga actgcaccgc gccttcccgg aagcggtctt gtttattcac ccgctggatg
     3241 cgaaagcgcg cgatctgcgc cgtggcgaca aagtgaaagt ggtttctcgc cgtggcgaag
     3301 tgatctcgat tgttgaaacg cgcggtcgta accgtccgcc acagggcctg gtgtacatgc
     3361 cgttcttcga cgccgcacag ctggttaaca aactaacgct ggacgcgacc gatccgctct
     3421 cgaaagagac ggacttcaag aagtgcgcag tcaaactgga gaaggtgtaa gccattatgt
     3481 cccggtcagc gaaacctcaa aatggtcgcc gccgctttct gcgcgatgtt gttcgcacag
     3541 caggcgggct ggctgccgtg ggtgtggcgc tggggttaca acagcaaacc gcacgcgcat
     3601 ctggcgtgcg gttgcgcccg cccggagcca taaacgagaa cgcctttgcc agtgcctgtg
     3661 tgcgttgcgg tcagtgtgtt caggcttgcc cttacgacac cttaaaactg gcgacgctgg
     3721 cttctggcct gtcggcgggc acgccatatt ttgtcgcacg ggatattcct tgcgaaatgt
     3781 gtgaggacat tccgtgcgcc aaagtgtgcc cgagtggtgc gctggatcgt gagatcgaat
     3841 cgatcgacga cgcgcggatg gggctggcgg tgctggtgga ccaggaaaac tgtctcaact
     3901 ttcaggggct gcgctgcgat gtttgttatc gcgaatgccc gaaaattgat gaggccatca
     3961 ccctggagct ggagcgcaac acgcgtaccg gtaagcacgc ccgctttctg ccgacggttc
     4021 acagcgacgc ctgtaccggc tgcggtaagt gcgaaaaagt gtgcgtgctg gaacaaccgg
     4081 caatcaaggt gttaccgctg tcactggcga aaggggagtt aggtcaccat taccgcttcg
     4141 gctggctgga ggggaacaat ggcaaatcgt aaacgtgacg ccgggcgcga ggcgctggtg
     4201 aaaaaaggct ggtggcgcag tcaccgttgg ctggtgttac gtcgcctttg tcagttcttc
     4261 gtgctgggga tgtttttgag cggtccgtgg tttggtgtgt ggatcttaca cggcaactac
     4321 agctgtagcc tgttattcga caccgttccg ctgaccgatc cgctgatgac gctgcaaagt
     4381 cttgccagtg gtcatctgcc tgccacggtg gcgttgaccg gtgcggtgat aatcactgtg
     4441 ctctacgccc tggcaggaaa gcgattattt tgcagctggg tctgcccgct gaacccgatt
     4501 accgacttag cgaactggtt acgcaggcgc tttgacctca atcagtctgc gacgatccct
     4561 cgccacattc gctacgtgct gttggtggtc attctgctgg gatcagcctt aaccggcacg
     4621 ctcatctggg aatggatcaa ccccgtttcc ctgatggggc gtagcctggt aatgggcttc
     4681 ggcagcggcg cgctgcttat tctcgcgctg tttttatttg atctactggt cgttgaacac
     4741 ggctggtgcg ggcacatttg tccggttggc gcgctgtatg gcgtgctggg tagcaaaggt
     4801 gtgattaccg ttgcggccag cgatcggcag aaatgtaacc gctgtatgga ttgttttcat
     4861 gtttgcccgg aaccgcatgt gctacgtgcc ccggtgctgg atgaacaaag cccggtgcag
     4921 gtcaccagcc gcgattgcat gacgtgcggt cgctgcgtgg atgtctgttc tgaggatgta
     4981 tttacaataa ctacacgatg gagttcggga gcgaaatcat gaaaagccat gacctgaaga
     5041 aagcgctgtg tcaatggacg gcgatgctgg ccctggtggt aagcggcgcg gtttgggcgg
     5101 ctaatggcgt cgattttagc cagtcgccgg aagtatccgg gacgcaggaa ggggccattc
     5161 ggatgccaaa agagcaggat cggatgccgc tgaactatgt gaatcagccg ccaatgatcc
     5221 cgcatagcgt tgaaggttat caggtaacga ccaataccaa ccgctgcttg cagtgccacg
     5281 gtgtcgaaag ctatcgcacc actggcgcgc cgcgtatcag tcctactcac tttatggaca
     5341 gcgacggcaa agtgggcgcg gaagtggctc cgcgtcgtta tttctgtctg caatgtcacg
     5401 taccgcaggc cgataccgcg ccaatcgtgg ggaatacctt taccccatca aaaggttacg
     5461 ggaaataaga ggttattatg ggaaattctg accgtaagcc tggtctgatt aagcgcctgt
     5521 ggaaatggtg gcgtaccccc agccgtctgg cgctgggaac gctgctgttg atcggttttg
     5581 tcggcggcat cgtcttctgg ggcggcttta acaccggaat ggaaaaagcc aataccgaag
     5641 agttctgcat tagctgccac gaaatgcgta acacggtgta tcaggaatac atggattccg
     5701 tgcactacaa caaccgtagc ggcgtccgtg cgacctgtcc ggattgtcac gttccgcacg
     5761 agtttgtgcc gaagatgata cgcaagctca aagcaagtaa agagctgtat ggtaaaattt
     5821 ttggcgttat tgacacgccg cagaaatttg aagctcatcg tctgacaatg gcacagaatg
     5881 agtggcggcg catgaaggac aataactcgc aggagtgccg taactgtcac aacttcgagt
     5941 atatggatac aaccgcccag aaatcggttg ccgcgaagat gcatgaccag gcggtgaaag
     6001 atgggcaaac ctgtattgat tgccataaag ggatagcgca caagctgccc gatatgcgtg
     6061 aagtcgagcc aggtttttaa cagggttatt gcgtgggtat gcttgaagcc agagagttac
     6121 tttgtgagcg ggatgaacga accttattta gtggcttgtc atttacgctg aacgcaggag
     6181 agtgggtaca aatcaccggt agcaacggcg cggggaagac aacgcttctc cgtttgctga
     6241 cggggttgtc tcgccctgac gcaggcgagg ttctctggca agggcagccc ttgcatcagg
     6301 tacgcgacag ctaccatcaa aacctgttat ggataggcca tcagccgggg atcaaaaccc
     6361 ggctgacggc gttagaaaat ctgcactttt atcatcgcga tggcgatacc gcacaatgtc
     6421 tggaagccct ggcgcaggcc gggcttgccg gattcgacga tattccggta aatcaacttt
     6481 ctgccgggca acaacgccgc gtcgctttag cgcgtctgtg gctgacccgt gccacgttat
     6541 ggatcctcga cgagcctttt accgcgattg acgttaacgg cgtcgatcgt ctgacccagc
     6601 gtatggcgca gcatacggag caggggggga ttgtgattct gactacccac cagccgctca
     6661 acgttgctga aagtaaaatt cgccgcattt cactgacgca aacgagggcc gcatgatgtt
     6721 ctggcgcatt ttccgtcttg agctgcgtgt agcgtttcgc catagcgccg aaatcgccaa
     6781 cccgctgtgg ttcttcctga ttgtaattac cctttttccg ctcagtatcg gtccggagcc
     6841 gcaactgctg gcgcgtattg caccgggcat tatctgggtt gctgcgctgc tttcatcctt
     6901 gctggcgctg gaacgactgt tccgtgacga tttgcaggac ggcagtcttg aacaattgat
     6961 gttgttgccg ttacccttgc ccgccgttgt gctggcgaag gtgatggcgc actggatggt
     7021 aaccggtctg ccgttactca tcctttcgcc actggtagca atgctactgg gaatggatgt
     7081 ttatggctgg caagtgatgg cgctgacgct gctgctggga acgcctacgc ttggctttct
     7141 cggtgcaccg ggtgtggcgt tgacagtggg acttaagcgc ggtggtgtgc tgctcagcat
     7201 actggtgtta ccgctgacta tcccattact catctttgcc accgccgcga tggacgcggc
     7261 ttccatgcat ttgcccgttg acgggtatct ggcaatttta ggcgcgttgc tggcaggcac
     7321 cgcgacatta agtccttttg cgacggcggc agcgttacga atcagcattc aataacgctg
     7381 gattactgat ttttgttcga gtctggtatc gaaactatgt ggaaaacact gcatcaactg
     7441 gcgatcccac cacggctgta tcaaatctgt ggctggttta taccgtggct ggcaattgcc
     7501 agtgtggtcg tgcttaccgt cggctggatc tggggattcg gctttgctcc ggctgattat
     7561 cagcagggaa atagctaccg cattatctac ctgcatgtgc ctgcggcgat ctggtcgatg
     7621 ggcatttatg catcaatggc agtggcagcg tttattggcc ttgtctggca gatgaaaatg
     7681 gccaacctgg cggtggcggc gatggccccc attggtgccg tgtttacctt tattgccctg
     7741 gttaccggct ctgcatgggg aaaaccgatg tggggcacct ggtgggtatg ggatgcacgt
     7801 ctgacttctg aactggtgct gctgtttttg tatgtgggtg tgattgccct gtggcacgcc
     7861 ttcgacgacc gccgtctggc gggccgtgcg gcaggtatcc tggtgctgat tggcgtggtg
     7921 aatctgccga ttattcatta ctccgtggag tggtggaaca ccctgcatca gggatcaacg
     7981 cggatgcagc aaagtatcga tccggcgatg cgttcgccgc tgcgctggtc gatttttggc
     8041 ttcctgctcc tgtctgccac gctgacgctg atgcggatgc gtaatttgat tttgctgatg
     8101 gaaaaacgcc gtccgtgggt gagtgaactg atactgaaaa gaggccgtaa atgacccctg
     8161 catttgcttc ctggaatgaa tttttcgcaa tgggcggtta cgcctttttt gtctggctgg
     8221 cggtggtgat gaccgttatt ccgctggtgg ttttggtcgt gcactcggtg atgcaacatc
     8281 gcgcaattct gcgtggtgtg gcgcaacagc gggcgcgtga ggcgcgttta cgtgctgcgc
     8341 aacagcagga ggctgcatga atattcgccg taaaaaccgc ttgtggattg cctgtgccgt
     8401 gttggcaggg ctggcgctga ctatcggtct ggtgctatat gcgctgcgct cgaatatcga
     8461 tctcttttat acgccggggg aaattctcta cggcaagcgt gaaactcagc aaatgccgga
     8521 agtcggtcag cgtctgcgcg ttggcgggat ggtgatgctg ggtagtgtgc agcgcgatcc
     8581 caattcgctg aaagtgacct tcactattta cgatgctgaa ggctcagtgg atgtctctta
     8641 cgaaggcatt ttgccggatc tgttccgtga agggcagggc gttgtggtgc agggcgagct
     8701 ggaaaaaggc aatcatatcc tcgcgaaaga agtgctggcg aaacacgacg aaaactacac
     8761 gccgccagaa gttgagaaag cgatggaagc taaccaccgt cgcccggcga gtgtttataa
     8821 ggacccagca tcatgatgcc ggaaattgga aacgggctgc tgtgtctggc gttagggatt
     8881 gcgctgctgc tgtccgtgta tccgctatgg ggcgtggcgc gcggagatgc gcgcatgatc
     8941 gcgtcttccc gcttgtttgc ctggctgctg tttatgtctg tggctggcgc atttctggtg
     9001 ctggtcaatg ctttcgtggt caacgacttt accgtcacct atgttgccag ctactccaat
     9061 acccagcttc cggtgtggta tcgcgtggcg gctacctggg gcgcgcatga aggctcgcta
     9121 ctgctgtggg tgctgctgat gagcggctgg acctttgcgg tggcgatttt tagtcagcgt
     9181 attccgctgg atattgtggc ccgcgtactg gcgataatgg ggatggtcag cgtcggcttt
     9241 ttgctgttca ttctctttac ctctaaccca ttctcacgca cgctgccgaa cttcccgatt
     9301 gaagggcgcg atcttaaccc gctgttacag gacccggggc tgatcttcca tccgcctctg
     9361 ctttatatgg ggtacgtggg tttctcggtg gcgtttgctt ttgccattgc ttctttgctg
     9421 agcgggcgtc tggacagcac ttatgcgcgt tttactcgcc cgtggacgct ggcggcgtgg
     9481 attttcctga cgctcggcat tgtgctcggt tccgcatggg cctattacga actcggctgg
     9541 gggggctggt ggttctggga tccggtagaa aacgcctcgt ttatgccgtg gctggtgggg
     9601 actgcgctga tgcactcact ggcggtcact gaacaacgcg ccagcttcaa agcgtggaca
     9661 ttactgctgg caatcagtgc cttctcgttg tgtctgttgg ggactttcct cgtgcgttcc
     9721 ggcgtgctgg tatcggtaca cgcgtttgcg tctgatccgg cgcgcggtat gtttatcctc
     9781 gcctttatgg tgctggtgat tggaggttcg ctgctgctgt ttgccgcgcg tggacacaaa
     9841 gttcgctcac gcgtaaacaa tgcgctgtgg tcgcgggaat ctctgctgtt agcgaacaac
     9901 gttttgctgg tcgccgcgat gctggtggtg ttgctgggga cgctgctgcc gctggtgcac
     9961 aagcaactgg gactgggcag tatttcgatt ggcgaaccgt tcttcaacac catgtttacc
    10021 tggctgatgg tgccgtttgc gctactgctg ggtgtcggtc ctctggtgcg ctgggggcgc
    10081 gatcgcccac gtaaaatccg caatttattg attatcgcct tcatctcaac gctggtgctg
    10141 tctctgctgt tgccgtggct gtttgagagc aaagttgtgg cgatgacggt gctcggcctg
    10201 gcaatggcct gctggattgc ggtgctggca attgcggaag ctgcgctgcg tatttcacgc
    10261 ggcacgaaaa ccaccttcag ttattggggg atggtggctg ctcacctggg gctggcagtg
    10321 acaattgttg gcattgcttt tagccagaac tatagcgttg agcgtgatgt gcgcatgaag
    10381 tccggcgata gcgtcgatat tcatgaatat cgcttcacct tccgtgatgt caaagaggtg
    10441 actggcccga actggcgtgg cggtgtggcg actatcggcg taacgcgaga gggtaaaccg
    10501 gaaacagtgc tgtatgcgga aaaacgttat tacaacactg ccgggtcgat gatgaccgaa
    10561 gcggcgattg acggtggcat cacgcgtgac ctgtacgccg cgctcggtga agagctggaa
    10621 aacggcgcgt gggctgtgcg tctttactac aaaccatttg ttcgctggat ttgggcgggc
    10681 gggctgatga tggcgttggg cggactgctg tgtctgtttg atcctcgcta tcgtaagcgc
    10741 gtgagtccgc aaaaaactgc gccggaggct gtatgaagcg caaagtattg ttaattccgt
    10801 tgattatctt cctggcgatt gccgcggcgc tgctgtggca gctggcgcgt aatgccgaag
    10861 gggatgatcc gaccaatctg gaatcggcgc tcattggcaa gccagtgccg aagtttcgcc
    10921 tcgaatcact ggacaatccg gggcagtttt atcaggcgga tgtgctgact cagggcaaac
    10981 cagtgctgct taacgtctgg gcgacctggt gtccgacctg ccgtgcggaa catcaatatc
    11041 tgaatcagct ttctgcgcag ggcatccgcg tagtcggcat gaactataaa gacgatcgcc
    11101 agaaggcaat cagctggctg aaagagctgg gcaatcctta cgcgctaagc ctgtttgatg
    11161 gcgatggcat gttagggctg gatctcggtg tctatggcgc gccagaaacg ttccttattg
    11221 acggcaacgg catcattcgc tatcgccatg cgggcgatct caatcctcgc gtctgggaag
    11281 aagagatcaa gccgctgtgg gagaaataca gtaaggaggc cgcacaatga ggtttttatt
    11341 gggcatgctg atgctgatga tctccggctc agcgctggcg accatcgacg tgttgcagtt
    11401 taaagatgaa gcacaggaac aacagttccg tcagctcact gaagaactgc gctgcccgaa
    11461 atgccagaac aacagcattg ccgattccaa ctcgatgatt gccaccgacc tgcgccagaa
    11521 agtgtatgaa ctgatgcagg aaggtaaaag taaaaaagag attgtcgatt atatggtggc
    11581 gcgttacggc aacttcgtca cttacgatcc gccgttaacg ccgctgaccg tgctgctgtg
    11641 ggtgctgcca gtagtagcta ttggcattgg cggttgggtc atatacgccc gctcgcggcg
    11701 tcgggtacgc gtggtgccgg aagcgtttcc tgaacaaagc gtgccggaag gtaagcgtgc
    11761 cggatatgtc gcttatttgc cgggcattgt ggtggcgtta attgtggctg gcgtcagcta
    11821 ctaccagacc ggaaattatc aacaggtgaa aatctggcag caggccacgg cacaggctcc
    11881 ggcgttgctg gacagggcgc tggatccgaa agctgatccg ctcaacgaag aagagatgtc
    11941 gcgccttgcg ctggggatgc gtactcaact gcaaaaaaat ccgggagata tagacggctg
    12001 gattatgttg ggccgcgttg gcatggcgct gggtaacgcc agtatcgcca ccgatgcata
    12061 cgctactgcg tatcgcctcg atccgaaaaa cagtgatgcc gcactgggtt atgctgaagc
    12121 gttgacacgt tcatctgatc ccaacgacaa ccgcctcggc ggcgaactgc tacgtcagct
    12181 ggtgagaacg gaccatagca atatccgtgt gctaagcatg tatgcgttta atgcctttga
    12241 gcagcagcga tttggcgaag ccgttgccgc gtgggagatg atgttgaaac tcttacctgc
    12301 caacgatact cgccgtgcgg tgattgaacg tagtatcgcg caggcgatgc aacatttgtc
    12361 gccgcaggag agtaaataaa aagaggcccg atggtgatgc catcgggcta tttttattgt
    12421 gccccgcgtt gttgcaggaa cagaatggtg gccgccacgc gtgagcggac attgagttta
    12481 cgcagcagat ttcgaatatg aacttttact gtctgctcgg aaatattcaa cacagaggca
    12541 atctgtttat ttgacagccc ctgtgccagc tcgtgcagaa catccagctc gcgctccgtc
    12601 agcacgctga agggatcttc ttccgcgcca aacatttcac gttcacgtaa gtactgattg
    12661 acgcgttcgc taaagacttt gctgcctttc gctccggcac gaatcgcttc cagcaacact
    12721 tccgggtcgc tgtctttcag cagataaccg tctgcgcctg cgtctatcag cgcaaagaca
    12781 tcgctggagg catcggatac ggtcaggata ataatttgcg cggtaacgcc atccctgcgc
    12841 aaggcattga gggtatccag gccactcata cctttcatat tgagatccag caagatcacg
    12901 tcgatatcca gtctattcgc cagatcgatc gcgctcgcgc cgtcgcccgc ttcggcgacc
    12961 acttcaaagc caggatcaag ctccagtaac tgacgaacac cgcgtcgcat aagtggatga
    13021 tcatccacaa tcatcacctg aaaaggcgtt gcttcaggca tagtagtctc ctgaggtttt
    13081 attagaatca ttatgtttga gcgtagcgtt ggtaacgcta ccaggcgcgt gaatgttacc
    13141 ccattttatg tatggacatg tagtgaaaac tacaaccttg ccgttcgctt tggcatcatt
    13201 ttcattataa agacggaatt ttgcatcagc ttctcatcaa gaaaaacaac taaaaaatca
    13261 atcacaatac ttgtgcattt ctttaaacag tttttaaaaa ctgactccgg gtatggagct
    13321 atgggtattt tctgtaccca atgcttttaa cagcaattaa tttcatagga tgaaagctca
    13381 atgcatcaat ctggttctgt ttctctttgt cgttccgcaa tatctgttct ggtggctaca
    13441 gcgttatatt cacccatagc attggcatca acagttgagt atggtgagac agttgatggt
    13501 gttggcctgg aaaaagatat ccagctggtt tatgggaccg ccaataatac gaaaatcaat
    13561 cctggcggag aacagcatat aaaagaattt ggtgtaagta gtaatactga aattaacggc
    13621 gggtatcagt acattgaaat gaatggcact gcagaatact cagtattaaa tgatggttat
    13681 caaattgttc aaatgggtgg cgcggtaaac cagactacgc tcaataatgg agtgctacag
    13741 gtttatgg
//
LOCUS       DAZPDU010000116        13693 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZPDU010000116 DAZPDU010000000
VERSION     DAZPDU010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13693
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.117"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..710
                     /locus_tag="ACHURS_003021"
     CDS             <1..710
                     /locus_tag="ACHURS_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000135033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFR1238691.1"
                     /translation="KESANHNKVMELIHSDKLLTQEDREFIFTNYRGDGIGATGAFFT
                     PEILAWDFVLDADCTGDCIELCAGIGRLSYYQYLRNKPTHITCVELNPEYVMIGKRVL
                     PEAEWITGNALQYSPDRFYDVAYGNPPFGKISTSKAYTGRYKGSEFEYKVIEHASTFA
                     SYGAWIVPQGSAGFVYSGVHCYERRESCKYQKFVKDTGYELHPGVGIDTSIYRDEWNG
                     TKVICESVLVDDDGTNL"
     gene            1203..1949
                     /gene="mipA"
                     /locus_tag="ACHURS_003022"
     CDS             1203..1949
                     /gene="mipA"
                     /locus_tag="ACHURS_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFR1238692.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2039..2893
                     /gene="yeaE"
                     /locus_tag="ACHURS_003023"
     CDS             2039..2893
                     /gene="yeaE"
                     /locus_tag="ACHURS_003023"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFR1238693.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHRSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISSAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(2944..3828)
                     /locus_tag="ACHURS_003024"
     CDS             complement(2944..3828)
                     /locus_tag="ACHURS_003024"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFR1238694.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHNEDADGVALTFELTQSEETKKFCPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSISMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(3912..4907)
                     /gene="gapA"
                     /locus_tag="ACHURS_003025"
     CDS             complement(3912..4907)
                     /gene="gapA"
                     /locus_tag="ACHURS_003025"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFR1238695.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5249..5662
                     /gene="msrB"
                     /locus_tag="ACHURS_003026"
     CDS             5249..5662
                     /gene="msrB"
                     /locus_tag="ACHURS_003026"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFR1238696.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            5704..5976
                     /locus_tag="ACHURS_003027"
     CDS             5704..5976
                     /locus_tag="ACHURS_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFR1238697.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKANCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(6137..6778)
                     /gene="pncA"
                     /locus_tag="ACHURS_003028"
     CDS             complement(6137..6778)
                     /gene="pncA"
                     /locus_tag="ACHURS_003028"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFR1238698.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(6789..7805)
                     /gene="ansA"
                     /locus_tag="ACHURS_003029"
     CDS             complement(6789..7805)
                     /gene="ansA"
                     /locus_tag="ACHURS_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFR1238699.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(7972..9828)
                     /gene="sppA"
                     /locus_tag="ACHURS_003030"
     CDS             complement(7972..9828)
                     /gene="sppA"
                     /locus_tag="ACHURS_003030"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFR1238700.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            9989..10540
                     /locus_tag="ACHURS_003031"
     CDS             9989..10540
                     /locus_tag="ACHURS_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFR1238701.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            10657..11700
                     /gene="selD"
                     /locus_tag="ACHURS_003032"
     CDS             10657..11700
                     /gene="selD"
                     /locus_tag="ACHURS_003032"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFR1238702.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGHIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            11705..13666
                     /gene="topB"
                     /locus_tag="ACHURS_003033"
     CDS             11705..13666
                     /gene="topB"
                     /locus_tag="ACHURS_003033"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFR1238703.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERALDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLLQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDVVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
BASE COUNT         3481 a         3326 c         3586 g         3300 t
ORIGIN      
        1 aaaaggaatc tgctaatcac aataaagtga tggaattaat acattcagat aagctcctaa
       61 cacaagaaga tagggaattc atattcacca attatagggg ggatgggata ggggctacag
      121 gtgccttctt cacacctgaa attttagcat gggattttgt acttgatgct gactgtactg
      181 gtgactgtat tgaactatgt gctggtatcg gtcgcctcag ctactaccag tacttaagga
      241 ataaaccaac gcatattaca tgcgtggaac tcaatcctga gtacgtaatg atcgggaaga
      301 gggtactacc agaggcagaa tggatcactg gtaacgcact acagtactca ccagaccgtt
      361 tctatgatgt tgcctatggt aatccaccct ttgggaagat cagtacctca aaagcgtata
      421 caggccgtta taagggtagt gaatttgaat acaaggtgat tgaacatgca agtacctttg
      481 cgtcctatgg tgcatggata gtaccgcaag gttccgcagg ctttgtctac agtggggtac
      541 attgttatga gcgtagagaa tcatgtaagt accagaagtt tgtgaaggat actggctatg
      601 agctacatcc aggtgtaggc atcgatacaa gtatctacag ggatgaatgg aatggtacta
      661 aggtgatatg cgaatcggtg ttagtagatg atgacggtac aaatctgtaa ctaaattagt
      721 taagaaatca taaagatagt agtacttgca aatgttccca ttgatgaagt tatgcgtcct
      781 ttcgtttttt agcacaggtt aagagccgtt catatgaata gtagaggctc ccggaagagg
      841 taaactgaac gacgtctctt gctaaaacac agcaactctc atgccatttt atcgacgatg
      901 agaacaacgt gcccggtgat acaccttcaa aattaagcgt agatggcaaa tggaaaactt
      961 gcctgcacgg ctttcttaat ttcaatggca tatcaacaac tcatccataa attttgcata
     1021 attaatgtaa agacaaggct cgccagtaac gctaaattca tttggctgta agcgcggtgt
     1081 catccgcgtc aggaaaatta aacagttact ttaaaaaatg aaaacgtaaa aaggttgggt
     1141 ttcgatgtat tgacgggtaa actttgtcgc ccgctaaaca tttgtttaag gaatgattaa
     1201 ttgtgaccaa actcaaactt ctggcacttg gagtgcttat cgcaacgtct gcaggcgtag
     1261 cgcacgctga aggtaaattt tccctgggcg caggcgtcgg tgtcgttgaa cacccatata
     1321 aagattacga taccgatgtt tacccagtac cggtaatcaa ctatgaaggc gataacttct
     1381 ggttccgtgg cttaggtggt ggttactacc tgtggaatga cgcaacggac aaactttcaa
     1441 ttaccgctta ttggtcgccg ctttacttca aagcgaaaga cagtggcgat caccaaatgc
     1501 gtcacctgga tgaccgtaag agcaccatga tggctggtct gtcttatgct cactttaccc
     1561 agtacggtta cctgcgtacc accctggctg gcgataccct ggataacagc aacggcattg
     1621 tctgggatat ggcctggttg tatcgttaca ccaacggcgg cctgacagtg accccgggta
     1681 tcggtgtgca gtggaacagc gaaaaccaga acgaatacta ttatggcgta tcgcgcaaag
     1741 agtccgctcg cagcggtctg cgtggctata acccgaacga cagctggagc ccttacctgg
     1801 agctgagcgc cagctacaac ttcctcggcg actggagtgt ttacggtacc gcgcgctata
     1861 cccgtctgtc tgatgaagtt actgacagcc cgatggtgga taaatcctgg actggcctga
     1921 tttctaccgg gatcacctac aaattctgat aatgcatctt tccagggcat tttgtgcata
     1981 aaaacagggc gtcacgccct gttttgcatt acggtggtgc aatacctggg aggaaacaat
     2041 gcaacaaaaa atgattcaat ttagtggcga tgtctcactg ccagccatag ggcagggaac
     2101 atggtatatg ggcgaagatg ccagtcagcg caaaacagaa gttgctgcac tacgcgcggg
     2161 cattgagctc ggtttaaccc tcattgatac cgccgaaatg tatgccgatg gcggtgccga
     2221 aaaggtggtt ggggaagcat taaccggtct gcgtgataac gtctttctcg tctctaaagt
     2281 ctatccgtgg aatgctggcg ggcaaaaagc gataaatgca tgcgaagcca gtttacgccg
     2341 tctcaatact gattatctcg atctctactt attacacagg tctggcagtt tcgcttttga
     2401 agagactgtt gcagcgatgg aaaaattgat cgcccaggga aaaatccgcc gctggggcgt
     2461 ttctaacctt gattatgctg atatgcagga actctggcag ctgccggggg gaaatcagtg
     2521 tgccactaat caggtgcttt accatctcgg ttcacgaggg attgagtacg atctactccc
     2581 ctggtgccag caacagcaga tgccggtgat ggcttacagt ccattagccc aggccgggcg
     2641 gttgcgcaat ggactgttaa aaaacgcggt agtcaacgaa attgcacatg ctcacaatat
     2701 cagctcggca caagtattgt tggcgtgggt aatcagccat cagggtgtga tggcgattcc
     2761 aaaagcggcc acgattgccc atgttcaaca gaatgcggct gtgcttgagg tcgaactttc
     2821 gtcagcggaa ttaaccatgc tggataaagc gtatccggca ccaaaaggaa aaactgcgct
     2881 ggatatggtg tgatgttgat acgtgccgga tgcatcgcac ccggcacaca acattaacgc
     2941 aaattaacgt ttcgcaacgc gaatggattg cgccagatgt gcaggtttct ctttggtgac
     3001 tttttgcgtt tctgaagcgt aagccgtttc tacacaaaca aatgttttgt agccatcatc
     3061 cggcatatcg cccatgctaa ttgaaagcgc cggtcccggg ttccagccta caacgttcag
     3121 atgatgctgg tggcctacgg cgataatacg attcagcgct tcatcattaa tcacgctgca
     3181 atcttgcgga ttcagataca cgcgatcggt acggtcaggg aaggtctgaa taccgtcggt
     3241 cagtacatct tctttcgcgt cattcacttt atcaatgaag cgatcgccca gcccactgac
     3301 gcttacctta gcgatatcac ccacgttaaa gtaggtatgc agggcagagg tggtttcaaa
     3361 ttcgccatgt gattcaagat cgatttcaca ggttttaccc acgcggaaat gcgctaacag
     3421 cgtaaagtcg tgcggacaga attttttcgt ctcttcgctt tgcgtcaatt caaaagtcag
     3481 cgctacgcca tcagcatctt cattatgtga tttcagcgtc cacggcaggt tgcgggcaaa
     3541 accgtgcgca ggcagacctt gttgtgccgc cggaccaaac cacggccagc aaaccggtac
     3601 gccaccgcga atagcgacgc catttttgaa cggtgtgttg ttgctcaacc acagaacttc
     3661 ttcttcaccc gcaggtttcc acgagagaag gtgtgcgccc tgtaatgcaa aagaggcttt
     3721 tacttgggga tgatcgacca caatgaggtc cagttcatcc agtttacgac gggagaggac
     3781 aggggagatt tgttcgatga ccggaagggc aaaaattttc ttaatcatga cgcagtcctt
     3841 taacttcatt ttatcaggta aaaaaaagag cgaccgaagt cgctcttttt agatcacagt
     3901 ttcatctcaa cttatttgga gatgtgagcg atcaggtcca gaactttgtt ggagtaaccg
     3961 gtttcgttgt cgtaccagga taccagtttc acgaagttgt cgttcagagc gataccagct
     4021 ttagcatcga acacggaagt gcaaacttcg ccgttgaaat cggtagatac tacgtcatct
     4081 tcggtgtagc ccagaacgcc tttcatttcg ccttcagcag cggctttaac ggcagctttg
     4141 atctgctcgt aagttgcagc tttttccaga cgaacggtca ggtcaactac ggatacgttc
     4201 ggggtcggaa cgcggaacgc cataccagtc agtttgccat tcagttctgg cagtacttta
     4261 cctacagctt tagcagcacc ggtagaggac gggatgatgt tctgggaagc gccgcggccg
     4321 ccgcgccagt ctttgtgaga cgggccatca acggttttct gagtagcggt agtagcgtga
     4381 acggtggtca tcagaccttc gatgatgccg aagttatcgt tgataacttt agccagcgga
     4441 gccaggcagt tggtggtgca ggaagcgttg gaaacgatgt cttggccagc atatttgtcg
     4501 aagttagcgc ctttaacgaa catcggagtg ttgtctttag acggaccagt cataaccact
     4561 ttcttcgcac cagcggtgat gtgtttacga gcagtttcgt cagtcaggaa cagaccagtt
     4621 gcttcagcga caacgtcaac accaacttcg tcccatttca ggttagccgg atcacgttca
     4681 gcggtaacac ggattttttt accgttaacg atcagatgac cgtctttcac ttcaacggta
     4741 ccgtcgaaac ggccgtgagt ggagtcatat ttcagcatgt atgccatgta atcagcgtct
     4801 aacaggtcgt tgattgcaac gatctcgatg tcagaacgtt tctgagcagc acggaaaaca
     4861 atgcgaccga tacggccaaa accgttgata cctactttga tagtcatata ttccaccagc
     4921 tatttgttag tgaataaaag gttgcctgta aaattacaaa aaccttacgc agcgtcaagc
     4981 ggaatcgtgt caatcattgc gacaaatcaa tcctgtgcct aagcattacg cgactgactc
     5041 gcctcactct tcctttgggc ttgaaaccac atggggtcgg cgccccgaat tttaaagggc
     5101 aattacgata aaaatgtgat tttcatcacg atttaggtgc agccatttcg tcgagatcaa
     5161 gtttaggaca aaattttgcc agttggtgtt aatgttttgt tagaatcagt cacgtaatgt
     5221 gagcacgatt aaagtgagat gtgagcaaat ggctaataaa ccttcggcag aagaactgaa
     5281 aaaaaatttg tccgagatgc agttttacgt gacgcagaat catgggacag agccgccatt
     5341 tacgggtcgt ttactgcata acaagcgtga cggcgtatat cactgtttga tctgcgatgc
     5401 cccgctgttt cattcccaaa ccaagtatga ttccggctgt ggttggccca gtttctacga
     5461 accggtgagt gaagaatcca ttcgttatat caaagacttg tcacatggaa tgcagcgcat
     5521 agaaattcgt tgcggtaact gtgatgccca tctggggcat gtcttccctg acgggccgca
     5581 gccaacgggc gaacgttatt gtgttaactc tgcttcttta cgcttcactg atggcgaaaa
     5641 cggcgaagaa atcaacggtt gaaaaaacga ttcagcaact tattccacag gagcggaaaa
     5701 attatgaatc ttgatgacat tatcaacagc atgacgcctg aggtatacca gcgtttgtcg
     5761 accgccgttg aactggggaa atggcccgat ggcgttgcgt tgaccgagga acaaaaagcg
     5821 aactgcctgc aactggtgat gctgtggcaa gctcgccata ataccgaagc acagcatatg
     5881 acaattgaca caaatggtca gatggtcatg aagagcaaac agcagttaaa agaagatttt
     5941 ggtatctcag caaagcctat tgctatgttt aagtgaatga gttatctagt atttaagcct
     6001 gttattaagg gagggcaaat atacttgtta ggacgtcgtg ttttagctgc aagtaggtta
     6061 aatgcaacac tgacaagtat tatctggcct acaatgtcat tgaggaaatg taggccagag
     6121 atgaattcaa ctaatattac ccctgtgtct cttcccagtc tgccagcgta tatagcgttg
     6181 ccccagctgc tgacatctcc ataaacgcgt gcgcactgtc ctggggctgg atattcacgc
     6241 cacgacaacc atcggtaatc acatttacct tataacctaa ctgtaacgcg tccagcacgg
     6301 taaacttcac gcaatagtca gtagccaggc ccataacgat caattcatcg atttcatgat
     6361 cgcgtaacca gtcatcgagc gaggttttct gccgacggcc gttatcaaaa aaggcactgt
     6421 aactgtcaac taaaggattt tcgcctttat ggaacaccgc tgcgatcgct ttttggttca
     6481 gtaacggatg taattgtgcg ccttcactgt tctgcacaca gtgatctggc cagaaggttt
     6541 gtggcaaacc gtcgagttgg cctggcgtat aaggctctac accgtgctga ctggcaaaac
     6601 tgccgtgatt cgccgggtgc cagtcctgac tggcgataac cgcttcaccg cgcgactggc
     6661 accagtcaat caggcggtta gcgacatcca ccgtactgtc accttccggc acggcgagcg
     6721 cgccaccagc acagaaatca ttttgtaaat cgaccagtaa cagggcgcga gggggcatta
     6781 cagtctcctt aatcatccgg cgtcagttcg ccgcgcaggt tttggctcat ggccttgcga
     6841 atggtttcag tatccagttc ctggctcagc aggtaatgca gtttggttag cgtggcttct
     6901 acagtcatat ctgcaccgcc aataacgccg gcatgggcga gggcgttacc ggtggcataa
     6961 ccacccatat tcactttacc ggacatacat tgtgtcaggt tgaccaccac aataccgcga
     7021 tcgctggctt cttgtaattc ctgcaggaag gctttgtttt gtggcgcatt acccacgcca
     7081 taggagcgca gaatcaatgc tttcaccggt tggcgcagaa aattgcgcac gacgtcagca
     7141 gaaatccctg gataaatcgt cactacgcca attggttgtg gcgtgattgg atgaacgatt
     7201 aattcccctt caccgtgcgg ggcgggtggc gtattcaaac gacgaatatg gataccagct
     7261 tccagtaacg gaggaaggtt tggagaggca aacgcatcaa aaccatcggc atgggctttg
     7321 gtagtgcggt tgccgcgata caatcggtta ttgaaaaaga gcgttacttc gttgatcgga
     7381 taattcgccg caacgtacaa cgcattcagc agattaattt gtccgtcaga gcgtaactca
     7441 gccagcggga tttgtgaccc tgtcacaatg accggtttac cgagattctc gagcatgaac
     7501 gacagcgcag aggcagtata cgccatcgtg tcggtgccgt gcaggatgac aaaaccatca
     7561 tagtcgtcat agtgcgcttt aatatcttca gcaatatgct gccagtcttc tggcgtcata
     7621 tcagaagaat ccatcagcgg cgtatattca tgaatggtga aatctggcat ctccgggcga
     7681 tggaattccg gcatcagcgc cagttggcgt tgtagatgac ctgacaccgg aatataaccc
     7741 tgctcggaac gctgcatccc gatggtcccg cccgtgtagg cgacgtaaat tgatttcttt
     7801 tgcatgatat tgatatgaat gatatcgaaa gagcaaaagt atatacgtga ggcaggagga
     7861 gcgcacctga tactatgcgg ataaggaaat atggggcgag acagcgggaa gaggattcgt
     7921 cgacgtaaaa aataatgtgg ccgacgatct gtcggccact cagtacaaga cttaacgcac
     7981 gttggcgcag gtcaggcaaa acgcataacg gttttgtggg tcgttaaacg cggccagctt
     8041 gtcgctttca cttttaacgg tagaggctac cgaggcaagc ggtgcaggta acatggcctg
     8101 gaacgcatct ggcaacattg cccggacaga accagacatg ttgtccatca ctttgtcgaa
     8161 gaaggtcggt tcatcaacgt agtattccag atgccactgt ttcacttttg ccagctctgc
     8221 tgctttggcg accgcatcat cgaaatcccc gagactatcg accagcccgt tagcttttgc
     8281 atcctgaccg gtccagacgt ggccctgggc gattttatca atctgctccg gcgtcgaatg
     8341 acgcgcatca gcaaccagcg tgataaagcg tttatagcca ttctcaatgc ttaattgcat
     8401 catctgctgc gcttccggcg gcagtgccct ggtgatagaa acatccgcca gcggtgaagt
     8461 tgagacacca tcagtatgaa caccaatcga atccagacta ttttctacgg tggtgatcac
     8521 gccgaagata ccgatagaac cggtcagggt gctggggtta gccacaatgt aattagctgg
     8581 cgtggaaatc cagtaaccac cagatgccgc catgccgccc atcgatacaa ccacaggctt
     8641 acccgctgcc cgggctgctg ccagttcagc gcgaatcact tcagacgcgg taacgctgcc
     8701 gcctgggcta ttaacacgca ggacaatcgc tttcactttc gggtcaaggc gagcgtcgcg
     8761 gatttgtgcc gcagtggtat caccgccaac attcccctga gtttcctcgc catccataat
     8821 tgcgccatta gcgaagacga caccgatgct gtcaccggta tctgccggcg ttttcaatgc
     8881 gtaatcgtaa taactgatgg cgcgataatt tttatcagtc ttactccagc cgaactcttt
     8941 ggttagagct ttttcgattt cggcactcga tgccagtgca tcgaccagct tgttttccag
     9001 tgcatatttc gcggtatcgc caccggtttt ggttaaaccc tcaagcaacc cttgcgcgcc
     9061 agggaatacc tgctgagcag ggatctgccg gttagcggca acagtattca gatagttttg
     9121 ccacagctca ccaatccagc ggctgtcagc ttcgcgggct gccggtgaca tgtcatcacg
     9181 aataaacggt tcaacggcag atttatacgt acccacgcgg aacacatggg tggaaacttt
     9241 cagcttatcc agcaacgatt tgtagtacag accgttggta gcaaaaccgt gcaaatccac
     9301 cacgccttgc ggtgacagcc aaattttatt ggcgaaactg gcgagataat attgcccctg
     9361 gctgtagttc tcgccaacgg cataaaccgg tttcccgctg tcacgaaact ctttcagggc
     9421 tttgccgatg tactgcatag acggctggtc gccgcctgcg aagtttttca gatccatcac
     9481 gataccggtg atattgcggt cgtccttcgc ctggcgaatg gtgttgacga tatcaaacag
     9541 tgagttttcc tgcagacgat cggaactggc accaagcagc tggcggctta atttactaaa
     9601 ccgctgagaa ctgtcgggtt tatcgacgat cacaccagaa atgtccagca gcagtgcgcc
     9661 acgactggcc gtttctttcg aatcaccacc actgacctgc atccagatcc ccacaccaac
     9721 cagtacgagg aaaataaaga acaggttaag taccatttca cggacgaaat tcagcagacg
     9781 ccacgtccat ttaaaaaatc cggcaataaa tcgccaaagg gttcgcatgt attctcccaa
     9841 cttaaggtca cacctgtcac aggcgcaata gatgtggtta tcgtaatgac ccaaccggca
     9901 cttgtcagca ggaattgcgg actgtgctgt aacaaaatcc actctcgtgt taattttgtg
     9961 aataaatatc acgacaggag ttaatcaaat ggatgcactc gaactattga tcaatcgccg
    10021 tagcgcctcc cgcctggctg aacccgcgcc aacgggtgaa caactgcaaa acatcctgcg
    10081 tgcgggtatg cgtgcgccgg accataagtc catgcaaccg tggcattttt ttgtgattga
    10141 aggggaaggg cgcgagcgtt tcagcgccgt actggaacag ggggcgattg ctgccggtag
    10201 tgatgacaaa gctatcgaca aagcccgtaa tgcgccgttc cgcgcaccgc tcatcatcac
    10261 ggtggtggcg aaatgcgaag aaaatcataa agtcccgcga tgggaacagg aaatgtctgc
    10321 cggatgcgcg gtcatggcga tgcaaatggc agccgttgcc cagggatttg gcggcatctg
    10381 gcgcagtggc gcattaactg aaagtccggt agtgcgtgaa gcattcggtt gccgtgagca
    10441 ggataaaatt gtcggttttc tctacctcgg cacgccgcag ttgaaagcat ctacgtcgat
    10501 taacgtcccg gacccgacgc cgtttgtaac ttatttctga taattgtcgc gaaactgtct
    10561 ggattgtgag caaaggcgca ggaattcaga cactctcact tatcacttca cggaatgagg
    10621 gttaccatac ccggattgca ttgacaggag atgtccatga gcgagaactc gattcgtttg
    10681 acccaataca gccacggagc tggttgcggc tgtaaaattt ccccaaaagt gttggaaacc
    10741 atcctgcaca gtgagcaggc gaagtttgtt gatccgaatt tgcttgtggg taatgagacc
    10801 cgcgacgatg cggcggtgta cgatctgggc aatggcacca gcgttatcag taccaccgac
    10861 ttctttatgc cgatcgttga taatcctttc gattttggcc acattgcggc gactaacgcc
    10921 atcagcgata tcttcgcgat ggggggcaaa ccgattatgg cgattgcgat cctcggctgg
    10981 ccgattaaca aactttcccc ggaaattgcc cgcgaagtaa ccgaaggtgg acgctatgca
    11041 tgccgtcagg cgggtattgc gctggctggc ggtcactcca tcgatgcgcc ggagccgatt
    11101 tttggtctgg cggtaacggg gatcgtaccg accgagcggg tgaagaaaaa cagtaccgca
    11161 caagccggat gcaaactgtt cctgacgaaa ccgctgggga tcggcgttct taccacggct
    11221 gagaaaaaat cactgctgaa accagaacat cagggactag cgacggaagt gatgtgccgg
    11281 atgaacatcg caggcgcgtc ctttgccaac atcgaaggcg taaaagcgat gaccgacgtt
    11341 acgggttttg gtctgctggg ccatttgagc gaaatgtgtc agggcgcagg tgtgcaggcg
    11401 cgcgttgact atgacgcgat cccaaaattg ccgggggttg aagagtacat taagttgggc
    11461 gcagtgcctg gcggcaccga acgtaacttt gccagctacg gtcatctgat gggtgaaatg
    11521 ccgcgtgaag tgcgcgattt gctgtgcgat ccgcaaactt ctggcggttt gctgctggcg
    11581 gtcatgccgg aagcagaaaa tgaggtcaaa gctacagccg ccgagtttgg cattgaactg
    11641 acggcaattg gcgaactggt gccagcgcgc ggcggtcgtg ccatggttga gattcgttaa
    11701 ttcaatgcgg ttgtttattg ccgaaaaacc gagtctggcg cgcgccattg ctgatgtcct
    11761 gcccaaaccg caccggaaag gcgatggctt tatcgagtgc ggtaatggtc aggtggtgac
    11821 ctggtgtatc ggtcacctgc ttgagcaggc gcagccagac gcctacgaca gccgctatgc
    11881 gcgctggaat cttgcggatt tgccgattgt cccggaaaag tggcaattac agccccgacc
    11941 ctccgtgacc aaacaactta acgtcatcaa acgcttcctg catgaagcca gcgaaatcgt
    12001 tcacgccggg gacccggatc gtgaagggca actgctggtg gatgaagtgc tggactatct
    12061 gcaactggca ccggaaaagc gccagcaggt gcagcgttgc ttgataaacg acctgaaccc
    12121 gcaggcggtt gagcgggcgc tcgaccgtct tcggtccaac agtgagtttg tgccattgtg
    12181 cgtttctgcg ctggcgcgag cgcgtgccga ctggctgtac ggcatcaata tgacccgtgc
    12241 gtataccatt ctcggtcgca atgccggtta tcagggcgta ctttccgtgg gacgcgtgca
    12301 gacacccgtg ctcgggctgg tggtgcgccg cgatgaagag attgaaaact tcgtggcgaa
    12361 agacttcttt gaagtcaaag cacatatcgt gacacctgcc gatgagcggt ttaccgctat
    12421 ctggcaaccg agcgaagcgt gtgaaccgta ccaggatgaa gaagggcgct tgttacatcg
    12481 tccactggcg gagcatgtgg ttaaccgcat tagtggtcaa ccagcaattg tcaccagcta
    12541 taacgataaa cgggaatcag aatccgcgcc gctgcctttt tcgctttcgg cgttgcagat
    12601 tgaagcggca aaacgctttg gtctgagcgc gcagaacgtg cttgatatct gccagaagct
    12661 gtacgaaacg cacaagctaa tcacttatcc gcgttccgat tgtcgctatt tgccagaaga
    12721 acattttgcc ggacgccacg cggtgatgaa tgccatcagc gttcatgcac cagatctgtt
    12781 gctgcagcca gtggtagatc cagatatacg caaccgctgt tgggatgaca aaaaggtcga
    12841 tgcacaccac gccatcatcc cgaccgcacg gagttcagcc atcaacctga cggagaacga
    12901 agcgaaggtc tataacctga ttgcccgtca gtacttgatg cagttctgcc cggatgtggt
    12961 gttccgcaag tgtgttatcg aactggacat tgccaaaggc aaatttgtcg ctaaagcgcg
    13021 ttttcttgct gaagcaggct ggcgcacgct gttaggcagc aaagagcgcg atgaagaaaa
    13081 cgacggtacg ccattgcctg tggtggcgaa aggcgatgag ttgctgtgtg aaaaaggtga
    13141 agtggtagag cggcaaaccc agccgccgcg ccattttacc gatgcaacac tgctttcggc
    13201 gatgaccggg atcgcgcgct ttgtgcagga caaagatctg aaaaagatcc ttcgtgcgac
    13261 cgatggtctg gggacagaag caacccgtgc cgggattatt gaactgctgt tcaagcgtgg
    13321 ttttctgacc aaaaaagggc gctatatcca ctctaccgac gccggaaaag cgttattcca
    13381 ttcgctgcca gaaatggcga cgcgaccgga catgaccgcg cactgggaat cggtgctgac
    13441 gcaaatcagc gaaaagcagt gtcgctatca ggactttatg cagccgctgg tggggacgct
    13501 atatcagctt attgatcaag ccaaacgtac gccggtgcgg cagtttcgcg gcattgtggc
    13561 tccgggcagt ggtggcagtg ctgataagaa aaaggctgca ccgcgtaaac gtagtgcgaa
    13621 aaaaagtccg ccagcagatg aagtcggaag cggggcgata gcgtaagcga gtgaatcttt
    13681 cgtgctattc gag
//
LOCUS       DAZPDU010000117        13478 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZPDU010000117 DAZPDU010000000
VERSION     DAZPDU010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13478
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.118"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..1739
                     /gene="adeD"
                     /locus_tag="ACHURS_003034"
     CDS             <1..1739
                     /gene="adeD"
                     /locus_tag="ACHURS_003034"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFR1238704.1"
                     /translation="HISRAEYQELLAVSRGDAVADYIIDNVSILDLINGGEISGPIVI
                     KGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVTFETATLPRGL
                     TTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCDVNGASFTLEQ
                     MLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGLGGKELNAYIT
                     AGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNSPQCMLCTDDR
                     NPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGLLAPGKQADIV
                     LLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQPVSASDFALQF
                     TPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVCFIAVLERYGQRLAPACGLLGGF
                     GLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNGQVQSHLPLPI
                     AGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPALKLTSQGLFDV
                     EKFAFTTLEVTE"
     gene            complement(1784..3175)
                     /gene="uhpT"
                     /locus_tag="ACHURS_003035"
     CDS             complement(1784..3175)
                     /gene="uhpT"
                     /locus_tag="ACHURS_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFR1238705.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3313..4632)
                     /gene="uhpC"
                     /locus_tag="ACHURS_003036"
     CDS             complement(3313..4632)
                     /gene="uhpC"
                     /locus_tag="ACHURS_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFR1238706.1"
                     /translation="MLPFLKAPADAPLMADKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(4642..6144)
                     /gene="uhpB"
                     /locus_tag="ACHURS_003037"
     CDS             complement(4642..6144)
                     /gene="uhpB"
                     /locus_tag="ACHURS_003037"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFR1238707.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLVLP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6144..6734)
                     /gene="uhpA"
                     /locus_tag="ACHURS_003038"
     CDS             complement(6144..6734)
                     /gene="uhpA"
                     /locus_tag="ACHURS_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFR1238708.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(6809..7099)
                     /gene="ilvN"
                     /locus_tag="ACHURS_003039"
     CDS             complement(6809..7099)
                     /gene="ilvN"
                     /locus_tag="ACHURS_003039"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFR1238709.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7103..8791)
                     /gene="ilvB"
                     /locus_tag="ACHURS_003040"
     CDS             complement(7103..8791)
                     /gene="ilvB"
                     /locus_tag="ACHURS_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFR1238710.1"
                     /translation="MASSGTTLTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDVATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPSPALIHVRIDAEEKVYPMVPPGAANTEMVEE"
     gene            complement(8897..8995)
                     /gene="ivbL"
                     /locus_tag="ACHURS_003041"
     CDS             complement(8897..8995)
                     /gene="ivbL"
                     /locus_tag="ACHURS_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFR1238711.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            9560..9649
                     /gene="tisB"
                     /locus_tag="ACHURS_003042"
     CDS             9560..9649
                     /gene="tisB"
                     /locus_tag="ACHURS_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFR1238712.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(9773..9847)
                     /gene="ysdE"
                     /locus_tag="ACHURS_003043"
     CDS             complement(9773..9847)
                     /gene="ysdE"
                     /locus_tag="ACHURS_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFR1238713.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            9929..11113
                     /gene="emrD"
                     /locus_tag="ACHURS_003044"
     CDS             9929..11113
                     /gene="emrD"
                     /locus_tag="ACHURS_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFR1238714.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(11121..11618)
                     /locus_tag="ACHURS_003045"
     CDS             complement(11121..11618)
                     /locus_tag="ACHURS_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFR1238715.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRCDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11615..11977)
                     /locus_tag="ACHURS_003046"
     CDS             complement(11615..11977)
                     /locus_tag="ACHURS_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFR1238716.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(11967..12314)
                     /locus_tag="ACHURS_003047"
     CDS             complement(11967..12314)
                     /locus_tag="ACHURS_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFR1238717.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            12423..12872
                     /gene="yidI"
                     /locus_tag="ACHURS_003048"
     CDS             12423..12872
                     /gene="yidI"
                     /locus_tag="ACHURS_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFR1238718.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFVGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(12919..13077)
                     /locus_tag="ACHURS_003049"
                     /pseudo
     CDS             complement(12919..13077)
                     /locus_tag="ACHURS_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13121..>13478)
                     /locus_tag="ACHURS_003050"
     CDS             complement(13121..>13478)
                     /locus_tag="ACHURS_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR1238719.1"
                     /translation="PGRNVRAKSGLNRSILDQGWYEMRRQLAYKQLWRGGQVLAVPPA
                     YTSQRCVCCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQS
                     GRSLKQEPTEMIQATA"
BASE COUNT         3168 a         3769 c         3532 g         3009 t
ORIGIN      
        1 atcacattag ccgggctgaa taccaggaat tgttagccgt ttcccgtggc gacgctgttg
       61 ccgattatat tattgataat gtctctattc tcgacctgat caatggcgga gaaatttccg
      121 gcccaattgt gattaaagga cgttacattg ccggtgttgg cgcagaatac gctgatgctc
      181 cggctttgca gcggattgat gctcgcggcg caacggcggt gccagggttt attgatgctc
      241 acctgcatat tgaatccagc atgatgacgc cggtcacttt tgaaaccgct accctgccgc
      301 gcggcctgac gaccgttatt tgcgaccctc atgaaatcgt caacgtgatg ggagaagccg
      361 gattcgcctg gtttgcccgc tgtgccgaac aggcaaggca aaaccagtac ttacaggtca
      421 gctcttgcgt acccgccctg gaaggctgcg atgttaacgg tgccagtttt acccttgaac
      481 agatgctcgc ctggcgggac catccgcagg ttaccggcct tgcagaaatg atggactacc
      541 ctggcgtaat tagcgggcag aatgcgctgc tcgataaact ggatgcattt cgccacctga
      601 cgctggacgg tcactgcccg ggtttgggtg gtaaagaact taacgcctat attactgcgg
      661 gtattgaaaa ctgccacgaa agttatcagc tggaagaagg acgccggaaa ttacaactcg
      721 gcatgtcgtt gatgatccgc gaagggtccg ctgcccgcaa tctcaacgcg ctggcaccgt
      781 tgatcaacga atttaacagc ccgcaatgca tgctctgtac cgatgaccgt aacccgtggg
      841 agatcgccca tgaaggacac atcgatgcct taattcgccg cctgatcgaa caacacaatg
      901 tgccgctgca tgtggcatat cgcgtcgcca gctggtcgac ggcgcgccac tttggtctga
      961 atcacctcgg cttactggca cccggcaagc aggccgatat cgtcctgttg agcgatgcgc
     1021 gtaaggtcac ggtgcagcag gtactggtga aaggcgagcc gattgatgcg caaaccttac
     1081 aggcggaaga gtcggcgaga ctggcacaat ccgctccgcc atatggcaac accattgccc
     1141 gccagccagt ttccgccagc gactttgccc tgcaatttac gcccggaaaa cgctatcggg
     1201 tcattgacgt catccataac gaattgatta cgcactccca ctccagcgtc tacagcgaaa
     1261 atggttttga tcgcgatgat gtgtgcttta ttgccgtact tgagcgttac gggcaacggc
     1321 tggctccggc ttgtggtttg cttggcggct ttggactgaa tgaaggtgcg ctggctgcga
     1381 cggtcagcca tgacagccat aatattgtgg tgatcggtcg cagtgccgaa gagatggcgc
     1441 tggcggtcaa tcaggtgatt caggatggcg gcgggctgtg cgtggtacgt aacggccagg
     1501 tccaaagtca tctgccgttg cccattgccg gcctgatgag caccgacacg gcgcagtcgc
     1561 tggcggaaca aattgacgcc ttgaaagccg ccgcccgtga atgcggtccg ttacccgatg
     1621 agccgtttat tcagatggcg tttctttctc tgccagtgat ccccgcgcta aaactaacca
     1681 gtcaggggct atttgatgtc gagaagtttg ccttcactac gctggaagtc acggaataat
     1741 aaaaaagccc ggcgtcatgc cgggcaaaag tcaccagtta cgtttatgcc actgtcaact
     1801 gctgaatttt tttctcgcgg cggattttgc gttcttccat caccgccact atcgccatca
     1861 ggcagataca accaatcgcg gcgatatcaa gtgcggcgaa ggtgcctgcc cagccggtaa
     1921 ggccgaatac cggcgtccca tcggcaatca ttcccagacc taacttggca aagctgtcac
     1981 caatcaggta agcaaaggtg cctttaatac catcggcagc gccaatcgct tttttaggta
     2041 caaagccaac agcagccaca ccaatcaaca attgcgggcc aaagaccagg aaacccaacg
     2101 caaagagaga agccagatag atatattcgt tactggcatg ttgatacaca ccgagcgtgg
     2161 cgataatcag cgccagcgcg atgcaggcca ccaggccacg gcgaccgttc gctaggtcag
     2221 agagccagcc ccacagcagc gtaccgacca gcgcaccagc ttcaaacagc gtaaagccct
     2281 gaatcgccac cgctttagag agtttcagtt cctggaacgc gtatacggtt gaccactggt
     2341 cgataccaat acgtaccaca tagaggaaaa tgttggcgaa gcacagcagc cagatcactt
     2401 tgtttttcag cacatactca acaaagatct gccacttggt catatcggta gattctgtct
     2461 ctttgtcctc ttcgctgatc tcctcgccga acagttcttc agctttgccg aggccataag
     2521 attccgggga gtcgctgccg taacgcaggc cgataaaacc gacaatcagc gcgataatcg
     2581 acgggaagat aaacatgccg atgacatggc catcgaacag gtaatttgcc ccgaacagcg
     2641 ccacgcctgc tgcgcctgca ccgccaaggt tgtgagaaat attccagaaa ccgaggaatg
     2701 tcccgcgttt acgacgcggc gtccatttgg tgatggtgga gtaactgcac gaaccgccgg
     2761 tactctggaa aaagccgctt aaggcgtaga aggcaatcat caggaacagg ctaaccgagc
     2821 cgctgcccat actggcactg aagcccagca tacaaatggc agacaggatc agcatgaacg
     2881 gcaggaattg tttggtgttt ttgccgtcgg cgtagtagga aaccagcgtt ttacccacgc
     2941 cataagtgat ggagaaaccc aggccgatca tccccagctg cgtcatgctc aacccgtagg
     3001 tcgagatcat gtcgttctgc gcgatgttaa agttcttgcg aatcaggtac atcgtcaggt
     3061 agccgataaa gaccaccagg taggattgca tgaacggttt gaaccacatt ttgcgccgca
     3121 cttcgagcgg aaggtccagg gtcggcttgc gaacctggtt taagaaagcc agcatgggtt
     3181 actcctgaaa tgaatacctg cgtgaggcat gcattgtaaa aatcagcagc aaatcaggcc
     3241 tgagatagcg tccaggcaaa acctggaaaa tttcttagtt ttgccggata tgaagtgaaa
     3301 aggtgagatg catcacgctt cgcgcggtgt ctgggcgttc aaaaagggca gtaacagcag
     3361 tgcggaaatc ccggcggcga tagagataac cacaaaaaat ccgctccagt gccaggtatc
     3421 gagtactttc gccagcggcc aaccagcaag cgacgccccc agataagcaa acaagccgac
     3481 aaaccccgtc gccgcccctg ccgcctcttt gtgggaacac tctgccgccg ccataccgat
     3541 taacatctgt gggccaaaga caaaaaaacc aatggtgaag aagcaggttg cctgcatcac
     3601 gtagctggca aatggcatca gccacaggga gccgactgaa agcaaaattc cggcggcgaa
     3661 aatcaaattc atcggccctc ggttgccgtt aaacaatttg tccgagcccc aacccgctac
     3721 cagcgcaccg ataaatccac ccagttcaaa catcgtcact gccgtattcg ccgtgaccag
     3781 atcgacgccc agcgtctcgg acatatacaa attgccccag tcgttgatcg ccgcccggac
     3841 cacatagacc agcacatagc aaaacgaaag cagccagata tacggattca gcaacacata
     3901 tttggtgagg atctctttac gcgtcagccc tgccccttct tgttgttgag ctatctccag
     3961 cgcgtcgtgt cgccattcac cgaccgccgg taaacctaac gcctgcgggc gatcgcgcag
     4021 ccgccagcag agaaaaattc ccacgacgat cgccatacaa ccagcaatca tcatcccggc
     4081 acgccagccg taatgtagtg cagccgctgc catcacaatg ggaatgagtg cgccgccgac
     4141 gttatgcgcc gtgttccata atgcccacca accaccgcgc tcggtacgtg aataccaggc
     4201 cgttaacaga cgcgcacaca ccggtgaacc ccagccctgg aaaaaggcgt tcagcaccca
     4261 gagtacggca aacgcccata gcgacgtcga gaagccaaac agaatgttga taatgcccgt
     4321 ggcgataagc cctatcccca taaaataacg ggcatttgag cgatcgctga caatgccgga
     4381 gacaaacttc gacacgccat aggtaatgta aaacagggtc gctaacaggc cgatatcgct
     4441 acggctgagc acgccgttag caaggatttc tggtacggcg gcgttaaaac ttttccgcgt
     4501 gaagtaaaac agcgcgtaac ccagccagat ggtcagcaga atatgccgac gccagtagcg
     4561 atagcgggca tcaatttcat atttatcagc cattaatggc gcatcggcag gcgctttcag
     4621 aaacggcaac atcatcaaac cttagacata gcgttgaggt agagaaacgc tgacacgcgt
     4681 gccgtgcaga caggaaatgt gtaatgtgcc acccagcgcc gttacgcgct cgcgcattcc
     4741 ggtgaggcca aaaccttgtt gcccggaacc cggcggcaaa ccgctgccat cgtcttcaat
     4801 aaccagcatc aaccgttcat cctgctgcca gccttgcagg gtgaccgcgc tggcatcagc
     4861 atgtttcaca atgttgttca gcccttcctg gcagacacga aacagcgtca cgcgctggtt
     4921 ttcgcttaac gctgattcat cgattcgcca ttcgagatgg ctgacaatac cgcgcccttc
     4981 cagctccatt tcccgcatca gtgagcggat ggcctgctcc agggtgagat catccaactg
     5041 gcgcggacgt aaccgaccca acaaacggcg caccgcgtcg taaacgccca gcgatagttg
     5101 ttcgatgagt tgcccgctct gcttcacgct ggcgttatct gccgccagcc gctgaacaat
     5161 gcccgcctga gtacgaatag cagtgatggt ctggccgata tcatcatgca gctcacgcgc
     5221 cacatcacgg cgcacgctct cttcggtttc cagcaaccgt tcagccagat gctgattgcg
     5281 cgccagttcc ttttgcagcg actggttaag ttcacgcaac cgctggatgc cagcgccaag
     5341 caacaaccct gtcagacttt gcaccagcag cgagagcaat aaatccaccg gatgatcgcg
     5401 ccaggtttga ctggcgatca gcgcgatggc gttcatcaac gtcgcaatca gcgccccttg
     5461 ccaaccatag tgccaggcca gcgcgataat cggcagcacc agacagaatg gcgtaaagcg
     5521 cgacagttcg tccggcaatc ccaactggag ccagagactg ataacaaaca gcagcaagta
     5581 ccagaccaga tgtcgcccgc gccagttgat tggctgagaa accagtgacg ggccgagcgg
     5641 cagccaggtg ttattggcga gatagtgcca gaacaccaga catatcgggg ccagcgtcag
     5701 gccgccagtt aaagtcagca acagcgcatt ccacgactct ttgccgtgcc aaagccaggg
     5761 cagcgactgc aacaacgccg ccgccgttaa cgccgccccc tgtaacagca aggtgcgcca
     5821 gtcacgctga tggcgatagc gcgagatcag cgctacgggc agtaacgtca gtaaactacc
     5881 gatcatcaat aacggaaaat gggttaaacc gaccgcctgc gttagccagt aaatcagcag
     5941 ccactccgca cccagcaata cgggccagta tccgcgcggg cattgcagca ttagccccag
     6001 acgcagacca aacggaaata acagcaccgc catatcaggg cgctcaacca gatgcaggct
     6061 gatactccac aggcaaaacc atgcggcaga gaagataaaa aagcaggcaa taacggtaat
     6121 taagcgggag aacaacgtct tcatcaccag ccatcaaaca tgcggcgcgc cagctctacg
     6181 tcgttactga cgcccagttt ttccatcaga ttggcgcgat ggacgtgtac cgttttcggt
     6241 gacaagccca gttcggcggc aatctctttc accgccattc cttgcgccag tttttccgcc
     6301 acctggcgtt cacgtttggt tagcgggtcc tgacgaccgg atgccagttt aatggcaata
     6361 tccggcgtca gataacagcc acccgtggca accgtatgca ccgcagcaat gagttcatcc
     6421 gggctacagc gtttggaaag aaagccgcgt gcccccgcgt taagcgcctg ctcaaccagc
     6481 gcaggactgt cgtgaacgga gagcataatc gtcgccatac ctttcggcag ctggcttagc
     6541 agctccagac cggagatatc gggcatggag atatcgcaaa tacacacctg cacaccgcgc
     6601 cccggcagcc ccgccagcgc ctcgcgcccc gaaccaaact cggcaactac ctgcaaatca
     6661 ggttccagcc ccagcagctg cgcaaagccg gagcggacga tgaggtgatc gtctataagg
     6721 gcaacggtga tcatggtctt gtcctggcgg gtaaaaaaaa acgcgcttac cttaacgata
     6781 agcgcgatgt tgttcaagcc ttgagcggtt actgaaaaaa caccgcgatc ttgttaaaca
     6841 tcgtcggatc ggactgatta cgctgcactt tcacgacatc ttccagctta tcgatttggc
     6901 ttatcatctg ctccagacgc tggtcgtcat tgaccagtag ccagatatgg cttttgtcgc
     6961 tgtcctgaat cggcagacaa agaatgcctt caacgttaaa agcgcggcgg gcaaaaaggc
     7021 cacaaacgtg ggtcattacg cccggatggt tgcgaacggt gagctccaga attacgttgt
     7081 catgagttgt gttttgcatg gcttattcct ccaccatttc agtattcgcc gcacctggcg
     7141 gcaccatcgg gtaaactttt tcttcggcat caatgcgcac atggatcagc gccgggctag
     7201 ggcgattgat gatttcctgc aatgcagcct gcggatcggc ttcgttattc aaatcacagg
     7261 tttcgaggcc gaatccggcg gcaatctgca taaagttgat tttgcccgga taggtggcgg
     7321 caaaaacgcc ttgctcgtag aacagactct gttgctgatg caccagcccc agcgcttcgt
     7381 tgttcatcag aatgattttg acatccagct gattttcact ggcggtcgcc atctcctgaa
     7441 tattcatcat caggctgccg tcgccggaga aacacaacac tttgcgatcc gggttcgcca
     7501 gcgccgcgcc aatcgccgca ggcaggccaa aacccatcgt gcccagccca ccggaggtca
     7561 gccactggcg tgggcgattg agcggataag cttgcgcggt ccacatctga tgctgaccca
     7621 catcggtggt gataatcgca ttgtcatcga cacaggcggc aacggcgttg atcaggccgt
     7681 aatggcttaa tggatcgcac gctttcggga ttggacacgg gaattcgcgc tgcaaatccg
     7741 ctaccaactg gtgccactct gcacgcggtt gcgcttccac ctgcgggatc aattgcgcca
     7801 gcacgtcatc aacatccgcc tgaatcgcca catgcggctg cttgatttta cccagctctg
     7861 cgcgatcgat atcgacatga ataattttgg cattcggaca gaactgctcg gttttgccaa
     7921 tcgcccggtc atcaaaacgc gcaccgagca caatcaacaa atccgcctcc tgcaaaatat
     7981 agttggtact gcgcacgccg tgcatcccca gcatacccag cgacaacgga tgcgcttttg
     8041 gcagcatgcc cagcgccatt aaagtcatgg tggtaggcag ttgcgctttc tccgccagtt
     8101 cacgcacccg cgcaggcgca ttgatcacac caccacccag ataaagcacc gggcgtttgg
     8161 cagcgttaat cattgtagct acgtcacgaa tgctttcttc gctaaaggcg ggtgcagcgg
     8221 ctttttctgc caccgcgggc tgagcttcaa tctcaaaaac cgccgtttgt acatccttag
     8281 gaatgtctat ccacaccggg ccagggcggc ctgattgcgc aatgcggaag gcatcgctca
     8341 tgacctgcgg gagttcttcg atatgtctga ccagatagtt gtgtttggtg atggggatag
     8401 agatgccgta ggtgtcgact tcctggaagg cgtcggtgcc gatcatcgag gcgggaacct
     8461 gaccagtgat gcaaatcagc gggatggagt ccagccgcgc atcggcaatg gcggtcacca
     8521 ggttagtcgc acccggtccg ctacaggcca tacagaccgc cggtttaccg tcggtgcgcg
     8581 ccattccctg agcgataaag cccgcgccct gttcatgacg ggccagaata tggcggattt
     8641 gcgtgctttg gcttaaggca tcgtaaacag gcaggataga accgcccgga atgcctgtca
     8701 caatcttaat gccctgctgt tccaggaaat gaacgataaa ttctgcgccg gtaaagcgct
     8761 tacgcgtcaa tgttgtgccc gaacttgcca tgctccagtc cttttcttct gggccgactt
     8821 tccgggaggt gcttaaacga aaaaccccgc ccggtttgcg ccgacggggt tttggaatcg
     8881 tgtgttgttc cagtccctac ggcgcattgc cgacgaccac caccacacgc acgacgacca
     8941 ctgcggcgga tggcgcagtt ggtagtagtt ttgcgttgaa catggaagta gtcattgggg
     9001 accttgttgg ttttgtgttt aacaatattt atacaagcac agctttacag gggagacaat
     9061 ggaaaatttt tcagcaaggg aaaattgagg ggttgatcac gttttgtact gaattgcaga
     9121 taacaaaaaa ccccgccgga gcgaggtttc gtcagtcgcc cgcggctggt aaccgcaaag
     9181 cacactgtat tatgtcaaca cagaaagtat acgtgttccg cgcagaacgc gcaatttcgg
     9241 cacgaatttt gacgtattta gtgcatagtt gagtatcgat cacagtttgc gttttgtcca
     9301 aatattactg tttatttata cagtaaactt ctataatatc actgtacgca atgtgttatg
     9361 cgggggccgc atcgttaccc ggcgcactaa gtcctggctg aaacgggtgg tgccgtcagc
     9421 gccttaaccc cgcgtgagca cactgtgtta tgtcaacaag cacaaagttt ctccttgaga
     9481 taccgcgtgc acaacagctg gcaacaggca gcggaaaggt acgtcagctg gcagtgctcc
     9541 tgaaccacag gagacgcgta tgagcctggt ggatatcgcc attcttatcc tcaaactcat
     9601 tgttgcagca ctgcaactgc ttgatgctgt tctgaaatac ctgaagtaat tcagattcaa
     9661 gtcgcaccaa aggggagcgg gtaaccgctc cccttttata tttagcgtgc gggttggtgt
     9721 cggatgcgat gctgacgtat cttatccgcc ctaccatctc tcccggcaac atttattgcc
     9781 gcttttgttt acatattctg ccgctaaaca actccccatt cctggcgtat atctggctaa
     9841 cattcatcaa tgtgatagat tcctctcccg catttatggg aatgcgtagt gacttactct
     9901 aattattttt ataaaagcat ccgtgataat gaaaaggcat agaaacgtca atttgttatt
     9961 gatgttggta ttactcgtgg ccgtcggtca gatggcgcaa accatttata ttccagctat
    10021 tgccgatatg gcgcgcgatc tcaacgtccg tgaaggggcg gtgcagagcg taatgggcgc
    10081 ttatctgctg acttacggtg tctcacagct gttttatggc ccgatttccg accgtgtggg
    10141 tcgccgaccg gtgatcctcg tcggaatgtc catttttatg ctggcaacgc tggtcgcggt
    10201 cacgacctcc agtttgacgg tattgattgc cgccagcgcg atgcagggga tgggcaccgg
    10261 cgttggcggc gtaatggcgc gtactttgcc gcgagattta tatgaacgga cacagttgcg
    10321 ccatgctaac agcctgttaa acatggggat tctcgtcagt ccgttgctcg caccgctaat
    10381 cggcggtctg ctggatacga tgtggaactg gcgcgcctgt tatctctttt tgttggttct
    10441 ttgtgctggt gtgaccttca gtatggcccg ctggatgccg gaaacgcgtc cggtcgatgc
    10501 accgcgcacg cgcctgctta ccagttataa aacgcttttc ggtaacagcg gttttaactg
    10561 ttatttgctg atgctgattg gcggtctggc cgggattgcc gcctttgaag cctgctccgg
    10621 cgtgctgatg ggcgcggtgt tagggctgag cagtatgacg gtcagtattt tgtttattct
    10681 gccgattccg gcagcgtttt ttggcgcatg gtttgccgga cgtcccaata aacgcttctc
    10741 cacgttaatg tggcagtcgg ttatctgctg cctgctggct ggcttgctga tgtggatccc
    10801 cgactggttt ggcgtgatga atgtctggac gctgctcgtt cccgccgcgc tgttcttttt
    10861 cggtgccggg atgctgtttc ctctggcgac cagcggcgcg atggagccgt tccccttcct
    10921 ggcgggcacg gctggcgcgc tggtcggcgg tctgcaaaac attggttccg gcgtgctggc
    10981 gtcgctctct gcgatgttgc cgcaaaccgg tcagggcagc ctggggttgt tgatgacctt
    11041 aatgggattg ttgatcgtgc tgtgctggct accgctggcg acgcggatgt cgcatcaggg
    11101 gcagcccgtt taagcgcacg tcaccgcagc atcgtcatca gctccatggg agaacgatgc
    11161 tgctttatca gatcacgcat cacccgcata tgcggtgcgg agtaagaata aaacgcctga
    11221 tagcccgcac aaagcacgct gtctggcgtc tcctgccgag cgtgcgggca caggcgatgc
    11281 cagtcgcacg cctggcagtc acagcgcggt tgcgggtttt cgctgccaca ccatttttgt
    11341 aaggtggcgt caaaaagctc aatcgagata cgactgatat ctgcccgcac ccaacactcg
    11401 aacagggcaa ttaaaaattt cccccacagc tctgctgtca ccgaagcggc cgtcagatcg
    11461 ccccggtcat caaattcaag atagggaatg agctgtacgg agcggatgcc ccgctgtttg
    11521 aaattgtgat aaaaatcggc aggttgcaga gcatccttgt actctacaac cagtttatgc
    11581 ctgtcctctt cagcatcaat gacctgactt cctgtcatgc gactctctca ataaatacga
    11641 taagttgatg tatatgcgtt acagcaaaca gtattgcgag agataacacc gcgagggaga
    11701 tcaaaaattt gtcacggact acgtgaaatt gggaaaaatc gctattcgtg acatccatta
    11761 aattgcgatt acgggtgtag tgccagagga tcagcgccac gatggcgaga atgccaatcg
    11821 aaatccagaa taacatgccc gcctggtgcc agttgtgttt gatagccaac gccatcaacg
    11881 cgccgtagcc cagcatggta cgaaaccacg ccagtgatgt gcgctccggc tgcagtcctg
    11941 ggtcggcaat gcgtctggct ttgcggctat ccggcataca acaccagtcc cataacgatc
    12001 accgccacga ccatcaatat taagctaatg attaataagc tgttggtgta gggcaggtcc
    12061 tctttcagac gcatcgcttt ttcattgcgt aaccagcgca ggtagccata cattgctaac
    12121 ccgccggaaa acagacacaa caacaacgcc agcagttcac gaatgacggg cgtggcgaaa
    12181 tctggcgcaa gttgatcaag cccaaccccc gccgccagaa atcctagtgc ggtacggatc
    12241 cacgccagaa aagtacgctc attagccagc gagaagcggt aatcgggcgc ttctccaagg
    12301 cgggaaattt tcattgttgc tcctttgcaa tttcactaac tctctacctt tagcggtttt
    12361 ttttaagaat ttcgtccgcc cgtttcctga tatgcttttg ccatccgcta caaggaggtc
    12421 agatgggtat catagcgcaa aataaaatca gttcattggg catgctgttc ggtgccatcg
    12481 ctttaatgat ggggattatc cacttttcat tcggcccttt ttccgcacca ccgccaacgc
    12541 ttgaaagcat cgtggcggac aaaacggctg aaattaaaag aggtcttctc gccggaataa
    12601 aaggcgaaaa aattaccacc gtggagaaaa aagaggacgt agatgtcgat aaaatcctcg
    12661 atcaaagcgg tatcgcactg gccattgctg cgctactttg cgcatttgtt ggcggtatgc
    12721 gtaaggaaaa tcgctggggc attcgcggcg cgctggtttt tggcggcgga acgctggctt
    12781 tccatacctt gctttttggt atcggcatcg tgtgcagcat tttgctgata ttccttatct
    12841 tttccttcct gaccggcggt tcgctggttt aagcagcctg aataatgccc gccgggtgat
    12901 catcagcggg cattatcgtc agaacttctg ttttttctct tccaccttca ccccttgtgt
    12961 cggtaggccg gtgtcatagt cacgtaccac cggcgaatag ccatcttgtg ggcgtggacg
    13021 aaatgccccc atccagcgcg gccgtgcatc tttacgccac ggacgcaggc tccattgttg
    13081 acatcctcca cgccctgaag gacgtggacc cctgctacgt tcaggctgtc gcctgaatca
    13141 tttcggtggg ttcctgcttc aacgagcggc ctgactgcac catctctcca caggcaagaa
    13201 cggcgtgccc cgccgctaaa atgttacgag cgccatttac atcggcgttc gctgtatatc
    13261 cacatacctg gcatctgaat ttactttgtg acaggcgatt ttctttcgct gtatgaccac
    13321 agcacacgca acgctggctt gtgtacgctg gcggaacagc aagcacctga ccaccacgcc
    13381 agagctgctt gtacgcaagc tggcggcgca tttcatacca gccctgatcc agtatcgaac
    13441 ggtttaaacc tgattttgcc cggacattgc gccccggc
//
LOCUS       DAZPDU010000118        13411 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZPDU010000118 DAZPDU010000000
VERSION     DAZPDU010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13411
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.119"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..81)
                     /locus_tag="ACHURS_003051"
     CDS             complement(<1..81)
                     /locus_tag="ACHURS_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HFR1238720.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTL"
     gene            complement(199..1638)
                     /gene="rsmF"
                     /locus_tag="ACHURS_003052"
     CDS             complement(199..1638)
                     /gene="rsmF"
                     /locus_tag="ACHURS_003052"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFR1238721.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQISARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(1719..4352)
                     /gene="yebT"
                     /locus_tag="ACHURS_003053"
     CDS             complement(1719..4352)
                     /gene="yebT"
                     /locus_tag="ACHURS_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFR1238722.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVNPSVVTL
                     LRENTRIELLNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(4321..5604)
                     /gene="yebS"
                     /locus_tag="ACHURS_003054"
     CDS             complement(4321..5604)
                     /gene="yebS"
                     /locus_tag="ACHURS_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFR1238723.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            5734..6231
                     /gene="msrC"
                     /locus_tag="ACHURS_003055"
     CDS             5734..6231
                     /gene="msrC"
                     /locus_tag="ACHURS_003055"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFR1238724.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6328..7026
                     /gene="proQ"
                     /locus_tag="ACHURS_003056"
     CDS             6328..7026
                     /gene="proQ"
                     /locus_tag="ACHURS_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFR1238725.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7046..9094
                     /gene="prc"
                     /locus_tag="ACHURS_003057"
     CDS             7046..9094
                     /gene="prc"
                     /locus_tag="ACHURS_003057"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFR1238726.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMRDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9286..10167
                     /gene="htpX"
                     /locus_tag="ACHURS_003058"
     CDS             9286..10167
                     /gene="htpX"
                     /locus_tag="ACHURS_003058"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFR1238727.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10213..11276)
                     /locus_tag="ACHURS_003059"
                     /pseudo
     CDS             complement(10213..11276)
                     /locus_tag="ACHURS_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            11453..12244
                     /gene="kdgR"
                     /locus_tag="ACHURS_003060"
     CDS             11453..12244
                     /gene="kdgR"
                     /locus_tag="ACHURS_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFR1238728.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(12387..12626)
                     /locus_tag="ACHURS_003061"
     CDS             complement(12387..12626)
                     /locus_tag="ACHURS_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFR1238729.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            12785..12928
                     /gene="mgrB"
                     /locus_tag="ACHURS_003062"
     CDS             12785..12928
                     /gene="mgrB"
                     /locus_tag="ACHURS_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFR1238730.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13003..13290
                     /locus_tag="ACHURS_003063"
     CDS             13003..13290
                     /locus_tag="ACHURS_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFR1238731.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
BASE COUNT         3489 a         3311 c         3458 g         3153 t
ORIGIN      
        1 taacgttcga tcacccggcg attcaccgtg taattcgccg tcgtcgattt caaaagcgcc
       61 tatgcgcaca ctggttttca tcttcgtgct ctccatttag ctgccggttg tggtacgacc
      121 agtccgatcg caccattttt cagcctagtt gaggcagccg aatgtgccag tcaaaaagtg
      181 cgcttttttt gtgcgctgtc aggcgttacc ggtaaaaagt ttcccatcgc gcaccagttc
      241 acgcggatag ctgtttttca atcgcgaacc aatccgtttg gctaaaccaa tcggttgatg
      301 ctggaaagta accaacacgt catccgccac tggcgcggct tgcgggtaaa catcgcgccc
      361 gcgataccac tcctccgctt cctgcggtgt cagttcaaaa gcgttcacat tgtcgggggt
      421 ggcaagggca ataactgctt catgctgcca gcgataacct ttgttgtgcg tttcggcaag
      481 tttaatcccc aagcgagaaa atcggacttt accgatcagg gcttcaatgc ccaccgggaa
      541 caaccacagt tctttgtcac gctgccagag gcgcaggttt tcatcccagt ttaagccaac
      601 acctgcagcc gcctgacgaa tttgtccagc ttcgcgatct ttcactgggc tgaacggaaa
      661 attaccgact ttgtatttgg gggcgggtaa ggcgggaatc gcctgagttt tacgcagacg
      721 agcaacgaag aagccttcgc agtcgtaaat ttgtgggaaa acatgcaaaa agccttcctc
      781 ggtcagcgcc ttgtttgcac cagggaagag atcaccaagc ggtaaaaact ctactgcgtc
      841 ggggtaagtc tctttcagcc acaggcaaac ggcttcgttt tcttcctggt ttaaggtaca
      901 ggtcgagtaa accagcgtac cgccagggcg taatgcatga aaggcgctgt cgatcagttc
      961 ccgttgggta gctgcgattt cctgattgct ttctggtgac cagtttttta gcgcatcggg
     1021 atctttacgc accacgcctt cgccggagca gggagcgtcc agcaaaatgg catcaaacat
     1081 ttctggcact gccgcaccaa acacgcggcc atcaaaatgt gtgagcgcaa cattactgat
     1141 accacagcgg ctgatattgg catgtaacac ttttacccga ctggcggaaa actcattggc
     1201 aagaattgcc ccttcgttat tcatccgcgc ggaaatttgc gtcgttttgg agccaggcgc
     1261 ggcagcgaca tccatcaccc gctgtggtgc attaccgtca gcaaacaagg cggcgacggg
     1321 caacattgaa ctggcttcct gaatataaaa caggccactt aaatgctcgg cggtactacc
     1381 caatggcaat gcatcttcat tgtcgcgttc aatccagaaa ccttcttcac accacggaat
     1441 tggcgtaagc gtccagccat aaggagcggt taattgcagg aaatcagcaa cggagatttt
     1501 cagcgtatta acgcgaatgc tgcggcgcaa cgggcgctga caggcggcaa gaaaatcatc
     1561 aaaagagagc gtcgaaggca tcgcttcgcg catttgcgtc agaaaggcgt ccgggaaata
     1621 aacggtgtgt tgggccacga gcatatacca ctggcaaaaa aaacaggcgc gcagtttagc
     1681 ataaacgctc cggcgcaggc acgccggagc agtgggcatt atttgggaag cgcagtaccc
     1741 cattcacgcc actctttcgg ttcactttcc tgcaacagga agtgcttgcc ttcctgggct
     1801 ttcggtgcca gtggcgtacc cggaggcgtg gcgaaggcga tgccgccacg gataaactgg
     1861 ttaaaggtgc cggttttcac tacgccgccc gtcagaccaa agtccagact gtaacccgat
     1921 gccaaccaga agacggaatt gttacgcacc aggtgttgat agcgtttact gatgcgcatc
     1981 gcaatcatca cgcgatctga caatgtcccc agcgtcattc cggtaaccgt acctacttcc
     2041 agaccacgga acagcacagg cgtaccaata cctaacgaac cggcttccgg tgcttcgaca
     2101 ataatgctta agccatccag gtaacgcgaa tcagtaatag tagcttcttg caactcaaag
     2161 tcacggcgag gattaccccg acctggttcg acattgatat acggctggag gatagtatct
     2221 agatgctcaa cgcccgcggc tgaaatttgc ggtgtgacca ccgagaagcg cgtaccgccg
     2281 cgggcgaagg tctggacata ctccggatag agcaccgcct ttgcctgtac ttcattgcgc
     2341 gcggtaatca gatccagcgt ctggatttgc ccgatatcaa taccgagata gcgaattggc
     2401 atcccgaccg ccagttttcc ggcatcgaaa gcgtgaagcg taatctgccc accaaccgca
     2461 cgggccgctg tttcggaagc atacagaatt cgtttgtcgc ctttacgctg actggcgctg
     2521 gcaccgctga ggttatcgaa gctaatggct ccctttaatg ctctggagag cggggatgcc
     2581 tgtacggtca ggccactacc attcagctga actttcgccc cgccttctgc ccagaacacg
     2641 ctattgctgg tcagaaggtt gcgatactcc ggcttaatat gcagatcgat atcaaacgcg
     2701 ttagctcgcg gacgcacggt aatcacttca ccaacttcaa atttacggta cagcactacc
     2761 gagcctgcct gcacatccgg cagcgtctct gcactcaaac tcacggttgt ggtgggtaaa
     2821 tcgctaaggc tgttctccag cgctttttcc agattggcat acagtggata gctggctttc
     2881 atctcacctt tatcgcctgg cagaatacgt atcccgccgt taatccattc tgaggcgctg
     2941 gcaccaagaa actcaacgcc atccagcccc accttcacgt cgacacggct gttgacgaca
     3001 aatttgctat cgccttttac cagttcgcga tgctgaggct cgatggcgac ggtaaaggta
     3061 acgcctttgc tggtgagttt acgatcaata acctggccta cctgcacgcc gtgaagaatg
     3121 agcggctgac cagcatcaat accgtaactt tccggcgcgg tcagggtcag cgtcagaaca
     3181 tcaggttcat gcagcagtgc tttttcgcct ggcacaacaa cgaactcttt gcgtggctcg
     3241 ccatcgccgg gtaccaactc gaaggttttg ccagtcagca gggcgctgag attggcatcg
     3301 ctaagggata atttcggatt aagtaattcg atgcgggtat tttcacgaag cagggtaacg
     3361 acgctgggat taactgtcat ttccccagtg actttaccac caggatttaa atccagttta
     3421 gttagctgtc cgacttccag tccctgatac attaatggcg tcgagtcggc ggttaatccg
     3481 gccccactcg gcagttccag tttgattatt acgccacgct ggctatgggc gaggtcttca
     3541 tacagaccaa aggtatcttc cgcctcggca ggtttcgact cttcaggtga atcgaaggca
     3601 atcgcaccgt taaccagtgc cgccagactt tccagtttca ccttcgcgcc actgatactg
     3661 acgttggcat caacgccgga aacgttccag aaacggctac cttttttcac caggtcggta
     3721 aaacgccgct cgatcaggac atcaatcacc acgccttgct tgttgggatt gatggcatag
     3781 tcgtagactt ttcccacagg gatcttgcgg aaatagacca atgaaccgct gttcagcgaa
     3841 ccgagatcgg gggcttgcag gtggatcatc agatcgccat tgtccagtcg atatttcggt
     3901 tgggtatcga gtgcgacaaa gtgatcctgc tctttacctt tacctggcat catgccgata
     3961 tagttaccac cgacgagggc gtccagcccg gagacacctg ccaacgatgc ttttggcgtc
     4021 accagccaga actgagtctc ttcgcgcagc gcatctttca tatcggactt gatgctgacc
     4081 ttgacttcaa tcttacgaag atcgtcgctg aggctgatat cctgcactgt tccgacttca
     4141 acgccctgat aacgaacagg cgtacggccc gggacaatac catccgccga cataaagtcg
     4201 atggtgacgg tattaccccg gtcctgataa ctgtcccaaa tcagccaacc ggcaatcatt
     4261 agcgcgatga aaggcagcag ccagaaaggt gagatacggc gtttattttt aatctgcgct
     4321 tcagtcgtcg aagcgggcgt ttcctgactc atgtgcatcc caaagtaagc ggctgtccag
     4381 ccattccaca gcaagaatag tcaaaattac ggctgcgcca aaataaaacg cagccggtcc
     4441 catagtaaaa gcgaggatct gatcgcgatt aatcagcgac atggttaaag atatgacaaa
     4501 caggtcgagc atcgaccagc gtccaatcca ggtcaccatc cgcagtaaca gaatgcgtgt
     4561 gcgtaaacct tgctggcatt taaaatgaat gctcaacagt aaagtgaaca tgacgatcac
     4621 tttagtaaac ggtaccagaa tactggcgat aaacacgatt ccggcgaccg caatgttgct
     4681 acttgccagc gacataatcc cggaaagaat cgtatcttcc tgccggccac cattcagata
     4741 aatgatagaa ataggcaata ggttggctgg taacaacaaa acgattgacg ctaacagcgc
     4801 cgcccagcat ttttgcaaac tatgacgacg acgcaggcgt agcgggatat ggcaacgcgg
     4861 gcagcgacca cgctgatcag gatagccggt aaaatggcac ccaagacaga cacgaagttt
     4921 ctcgtcccta cgcgtagcgg ggcgctgcgg ataatatcgc tcccacagct cctcaacatt
     4981 aagatgtgac aacgtcaccg tcgttaaaat caccaacgcc acaaaagaga acaagccgac
     5041 acccgcctgg atatgggcat aatcctgtac ctttatagaa gcaacgccaa tgccgaccag
     5101 gtagatatcc agcattaccc actctttaag acgctcaagc atcagcagta ccggacgtaa
     5161 attcattccc agtcggttac caaaccataa atatgctatg gaggacacca gaatgagggg
     5221 ggcaccaata acgcagaaaa agaccatcgc ccccgttatc gtatcgccct gtttggtcat
     5281 ttgccagatg ccttgcatga cgttggcgtc gatacgaata cctaacagcc agatatgcaa
     5341 cagcggttcg ccccaggcaa acggcatcag caacagcatg gtgaaagcca ttgccgccag
     5401 gcgcgttagc gaccagtcgc gcccgtcgcg aatttttgcc tgacagcgcg gacaataggc
     5461 actctgatga gaatttatct cgggcaggct aaacagcatg tcacattgcg ggcaacgttg
     5521 gtaatcacca cgcggcagtt cctcgccgat tgccctcact gttatctttt ttgtcggcgt
     5581 aatttgtggt gtgttaagag ccatgacgtg ccaaatgtaa taagaattaa cattaatctt
     5641 aacccatgat gggaatgatc ttgagcatta acgcatttaa tgcttatttt aataggctgg
     5701 cagttccagg taagacaaac aagtgattat ataatgaaca aaacagaatt ttacgcggat
     5761 ttaaatcgcg actttaacgc gctgatggcg ggagaaacca gttttctggc aacgcttgcg
     5821 aacaccagtg cgttgttata cgagcgtctc actgacgtaa actgggcagg tttttatttg
     5881 cttgaggacg atacactggt actcggacca tttcagggca aaattgcctg tgtccggata
     5941 cccgtcgggc gcggggtgtg cggcactgcg gttgcccgca atcaagtgca gcgtatcgag
     6001 gatgttcacg cgtttgacgg gcatattgcc tgtgatgcgg cgagtaattc tgaaattgtt
     6061 ctgccgctgg tggtgaaaaa tcagattatt ggtgttcttg acatcgatag taccgtcttc
     6121 ggtcgcttta cagacgagga cgagcaaggc ttacgtcagc ttgtggcaca gcttgaaaaa
     6181 gtgcttgcaa cgacggatta caaaaaattc tttgcgagcg tcgcaggata atcaacggat
     6241 aacgtagcat ttactggtag cgtcattata atgacgcctg ttcatgcctg cgcttgttgg
     6301 ctacgtccgt tgtaatcagg aaatttcatg gaaaatcaac ctaagttgaa tagcagtaaa
     6361 gaagtaatcg cgtttctggc cgaacgtttt ccccactgtt tcagtgcgga aggtgaagcg
     6421 cgtccgctga aaatcggtat ttttcaggat ttggtcgatc gtgttgccgg ggaaatgaac
     6481 ctgagcaaaa cgcaattacg atccgcttta cgtctctaca cttcgagctg gcgttatctt
     6541 tacggtgtta aacccggcgc aacgcgtgtc gatcttgacg gcaacccatg cggtgagctg
     6601 gacgagcaac atgtagagca tgctcgcaag cagcttgaag aagcgaaagc gcgtgttcag
     6661 gcacagcgtg ctgaacagca agcgaaaaaa cgcgaagctg ccgcagctgc tggtgagaaa
     6721 gaagacgcac cgcgccgcga acgcaagcca cgtccgacca cgccacgccg caaagaaggc
     6781 gctgaacgta aacctcgtgc gcaaaagccg gtagagaaag cgccaaaaac agtaaaagca
     6841 cctcgcgaag aacagcacac cccggtttct gacatttcag ctctgactgt cggacaagcc
     6901 ctgaaggtga aagcgggtca aaacgcgatg gatgccaccg tattagaaat caccaaagat
     6961 ggcgtccgcg tccagctgaa ttcgggtatg tctttgattg tgcgcgcaga acacctggtg
     7021 ttctgaaacg gaggccgggc caggcatgaa catgtttttt aggcttaccg cgttagctgg
     7081 cctgcttgca atagcaggcc agaccttcgc tgtagaagat atcacgcgtg ctgatcaaat
     7141 tccggtatta aaggaagaga cgcagcatgc gacggtaagt gagcgcgtaa cgtcgcgctt
     7201 cacccgttct cattatcgcc agttcgacct cgatcaggca ttttcggcca aaatctttga
     7261 ccgctacctg aatctgctcg attacagcca caacgtgctg ctggcaagcg atgttgaaca
     7321 gttcgcgaaa aagaaaaccg agttaggcga tgaactgcgt tcaggcaaac tcgacgtttt
     7381 ctacgatctc tacaatctgg cgcaaaagcg ccgttttgag cgttaccagt acgctttgtc
     7441 ggtactggaa aagccgatgg atttcaccgg caacgacact tataaccttg accgcagcaa
     7501 agcgccctgg ccgaaaaacg aggctgagtt gaacgcgctg tgggacagta aagtcaaatt
     7561 cgacgagtta agcctgaagc tggcaggaaa aacggataaa gaaattcgtg aaaccctgac
     7621 acgccgctac aaatttgcca ttcgtcgtct ggcgcaaacc aacagcgaag atgttttctc
     7681 gctggcaatg acggcgtttg cgcgtgaaat cgacccgcat accaactatc tttccccgcg
     7741 taataccgaa cagttcaaca ctgaaatgag tttgtcgctg gaaggtattg gcgcagtgct
     7801 gcaaatggat gatgactaca ccgttatcaa ttcgatggtg gcaggtggtc cggcagcgaa
     7861 gagtaaagct atcagcgttg gtgacaaaat tgtcggtgtt ggtcaaacag gcaagccgat
     7921 ggttgacgtg attggctggc gtcttgatga tgtggttgcc ttaattaaag ggccgaaggg
     7981 cagtaaagtt cgtctggaaa ttttacctgc tggtaaaggg accaagaccc gcactgtaac
     8041 attgacccgt gaacgtattc gtctcgaaga ccgcgcggtt aaaatgtcgg tgaagaccgt
     8101 cggtaaagag aaagtcggcg tgctggatat tccgggcttc tatgtgggtt tgacagacga
     8161 tgtcaaagtt caactgcaga aactggaaaa acagaatgtc agcagtgtga tcatcgacct
     8221 gcgtagcaat ggcggtgggg cgttgaccga agcggtatcg ctctcaggtc tgtttattcc
     8281 tgcgggtccc attgttcagg tccgcgataa caacggcaag gttcgtgaag acagcgatac
     8341 cgacgggcag gtgttctata aaggcccgct ggtggtactg gttgaccgtt tcagtgcttc
     8401 ggcttcagaa atctttgccg cggcaatgca ggattacggt cgtgcgctgg ttgtgggtga
     8461 accgacgttt ggtaaaggca ccgttcagca ataccgttca ttgaaccgta tttacgatca
     8521 gatgttacgt cctgaatggc cagcgctggg ttctgtgcag tacacgatcc agaaattcta
     8581 tcgcgttaac ggcggcagta cgcaacgtaa aggcgtaacg ccagacatca tcatgccgac
     8641 gggtaatgaa gaaacggaaa cgggtgagaa attcgaagat aacgcgctgc cgtgggatag
     8701 cattgatgcc gcgacttatg tgaaatcagg cgatttaacg gcctttgggc cggagctgct
     8761 gaaggaacat aatgcgcgta tcgcgaaaga tcctgagttc cagaacatca tgaaggatat
     8821 cgcgcgcttc aacgctatga gggacaagcg caatatcgtt tctctgaact acgctgtgcg
     8881 tgagaaagag aataatgaag atgatgcgac gcgtctggcg cgtttgaacg aacgctttaa
     8941 acgcgaaggt aaaccggagt tgaagaagct ggatgatcta ccgaaagatt accaggagcc
     9001 ggatccttat ctggatgaga cggtgaatat cgcactcgat ctggcgaagc ttgaaaaagc
     9061 cagacccgcg gaacaacccg ctcccgtcaa gtaatatcaa tcaggcacaa gaaattgtgc
     9121 ctgatttttt aacagcggca agatgccgta aatcagatgc tacaaaatgt aaagttgtgt
     9181 ctttctggtg acttacgcac tatccagact tgaaaatagt cgcgtaaccc atacgatgtg
     9241 ggtatcgcat attgcgtttt gttaaactga ggtaaaaaga aaattatgat gcgaatcgcg
     9301 ctcttcctgc taacgaacct ggccgtaatg gtcgttttcg ggctggtact gagcctgaca
     9361 gggatacagt cgagcagcgt tcaggggctg atgatcatgg ccttgctgtt cggttttggt
     9421 ggttccttcg tttcgcttct gatgtccaaa tggatggcat tacgatctgt tggcggggaa
     9481 gtgatcgagc aaccgcgtaa cgaaagggaa cgttggctgg tcaatactgt agcaacccag
     9541 gctcgtcagg cagggatcgc tatgccgcaa gtggctatct accatgcgcc ggacatcaac
     9601 gcttttgcaa ccggtgcgcg ccgtgatgcc tctctggttg ctgtcagcac cggtttgctg
     9661 cagaacatga gcccggatga agccgaggcg gtaattgctc acgaaatcag ccacatcgcc
     9721 aatggtgata tggtcaccat gacgctgatt cagggcgtgg tgaacacctt cgttatcttt
     9781 atttcccgta ttctggcgca gcttgccgcg ggttttatgg gcggaaatcg tgatgaaggt
     9841 gaagagagca acggcaaccc gctgatctac tttgcggttg caacggttct ggaactggtg
     9901 tttggtattc tggcgagcat tatcaccatg tggttctcgc gtcatcgtga attccatgct
     9961 gatgccggtt cggcaaaact ggttggtcgc gagaaaatga ttgccgcgct gcagcgcctg
    10021 aaaaccagct atgaaccgca agaagcaacc agcatgatgg ctttctgcat taacggtaag
    10081 tcgaaatcgc tcagtgagtt gttcatgact cacccgccgc tggataaacg aattgaagct
    10141 ctgcgtacgg gtgaatacct gaagtaatat gaagtaaata ctaaaaagcg cgtcctgatc
    10201 gacgcgcttt ttttatgccc tggatcgtgg ctgagtgata cgtaaaccac tgacacaggc
    10261 agcaatcact gccagaatag ccgcagccat cagcgaaacg tgcgtaccat tatcaccgaa
    10321 ctgatttagc atcagcgcca ccagtgccgc gccgctactc tgacccagta gacgagccgt
    10381 tcctaacatg ccactggctc caccgctacg ttcgcgagga gcggaggtaa taatggtgtg
    10441 gttattgggt gactggaaca agccaaatcc agcgccacat aagatcatcg gccagataat
    10501 attgatatcc gcaggtgacg cgggcagcag aaccagggga aaaagccccg cagccatgat
    10561 gaacaaccct agagcaccca gcaatcctgc atgtacgcgt tcaatcaaat agcctgccaa
    10621 cggagccatc accatcgttg ctaacggcca cggtgtcaga agtaaacctg tttcgacttc
    10681 actacgcccg agcacggttt gcaggtaaaa gggcagggaa accattgcca gcatttgtgc
    10741 gcagaaagag caaacagatg tgcaaataga aagtgaaaac agcgggatac gcagtaaatc
    10801 caccggtagc agcggtacgg gaagagaaag ctggcggcga ataaagaaaa taccaacaac
    10861 aaccattacc accagttccg cagcaattaa cgtcagcgat tgcccctgag cgaaaccact
    10921 caacgcagtg ataagcaggc cgaaggttaa cgcgttcatc acggcgctgg gcaggtcgaa
    10981 acggggttta ctggcgcgag aaccattggg tggcagaaaa cgcatcgcca gaagcagggc
    11041 gataatacct aacggtacgt tgattaaaaa taaccatttc caggatgcga tggagaggat
    11101 tgctgcagca attgtcggcc cggcagcaga agagacggca acaatatggc gattgttggc
    11161 agggcgacgt tggcgattgc gccgtcaagg acagccatcg aaatgccaat cacaatggtt
    11221 aatatcgcac cgtatcgctg gggcaatggc aggccgtcgg cctgaacttt tggcatagga
    11281 attttatatc tttggtgaat aatcaacagg ggtatgattc tagcactgat attcacggtg
    11341 gatgtcgcag aattgtaact aaccggcaaa agatggattg cgaccaccct ggtgcgaaat
    11401 tataataaaa aacagttctg atttttataa aacactcgca atgaggtgat aaatggctaa
    11461 cgcagatctg gataaacagc ctgattctgt atcttccgtg ctaaaagttt ttggcatttt
    11521 gcaggcgctg ggtgaagagc gcgaaatagg gataaccgag ctgtcgcagc gcgtcatgat
    11581 gtcaaaaagc accgtttatc gctttttaca gaccatgaaa accttaggtt atgtggcgca
    11641 ggaaggggag tcggagaaat attccctgac cctgaaattg tttgaactgg gcgctcgcgc
    11701 gttacaaaac gtcgatttaa ttcgtagcgc agatatccag atgcgtgaga tctcccgcct
    11761 gaccaaagaa actatccacc tcggcgcact ggacgaagac agtattgttt acattcacaa
    11821 aattgactct atgtacaatt tgcgcatgta ttcacggatt gggcgtcgta atccgctgta
    11881 cagcaccgcg attggtaagg tactgctggc atggcgcgat ctcgatgaag tgaagcaaat
    11941 tcttgagggc gtggagtata aacgcagtac cgagcggacc atcaccagta cagaagcgtt
    12001 attacccgtt ctggaccagg tgcgcgagca ggggtatggc gaagataacg aagagcagga
    12061 agaagggctg cgatgcattg cggtaccggt atttgatcgc tttggcgtgg tcattgccgg
    12121 tttgagcatc tccttcccga cgttgcgttt ctctgaagag cgtttacagg aatatgtcgc
    12181 aatgttgcat accgcagcgc gcaaaatttc tgcccaaatg ggttatcacg actatccgtt
    12241 ctgatgagag taagaacctg tcggaatatc aaacagacag gttctttatt tagcatgaga
    12301 aaaataaagt tgaaggtgcg ttatattaaa cgcgcttgct ataagaatat tttactcatg
    12361 agtgagaatc tggttgttta ttacccttaa ccattatcga ccacgatatt gcttttacgt
    12421 aacagtgggc aatctgttat ccccaagaaa ccacttttag tgtgcaagta ttgtaccgtg
    12481 ctggtgcctc tggcagtcag ataggtacat tgcaaaccta atcctgcggc attctctttg
    12541 cttccaatca aaacgccata tccgctgagt aataatccta tccataccag tgctatcagc
    12601 ataactgtgc gaatgatgaa tcgcattaca acctcttctc tttttatgtt cgcttaatcg
    12661 tagcggcaat atgcgctgaa gcaagctact cattccgaaa aagcacgaat atcgacatag
    12721 ttaggcgctg tttaactaac gcatgctagt ttaatgacat aaggtaggtg aagcggagat
    12781 tggagtgaaa aagtttcgat gggtcgttct ggttgtcgtg gtgttggctt gcttgctgct
    12841 ttgggcgcag gtattcaaca tgatgtgcga tcaggatgta caatttttca gcggaatttg
    12901 tgccattaac cagtttatcc cgtggtgata tcattttttc agcactagat tttcttcctc
    12961 tccagcagtg gtagaatagc gcctctactc agaggtggtg aaatgaacga agttgtaaat
    13021 tcaggcgtga tgaacattgc gtctttggtt gtatcggtgg tggttcttct tatcgggctc
    13081 atcttgtggt tttttatcaa tcgtgccagt tcacggacta acgaacagat tgaactgctt
    13141 gaggcgttgc tggatcagca aaaacgtcaa aatgcactgt tacgtcgttt gtgcgaagca
    13201 aacgaaccag agaaagcaga taaaaagacc attgagagtc aaaaatcggt tgatgacgaa
    13261 gatattattc gcctggtcgc cgaacgataa tccttcctca atcttgagta accccgtcgt
    13321 tgaagggtta ctcaattcat cctgattata cgcactgtca tggtgcaact gcttcaaatt
    13381 aaaactctgg tctgacttca aataattatc a
//
LOCUS       DAZPDU010000119        13334 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZPDU010000119 DAZPDU010000000
VERSION     DAZPDU010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13334
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.120"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..312)
                     /locus_tag="ACHURS_003064"
     CDS             complement(<1..312)
                     /locus_tag="ACHURS_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HFR1238732.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGSTTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LA"
     gene            complement(331..2208)
                     /gene="bglF"
                     /locus_tag="ACHURS_003065"
     CDS             complement(331..2208)
                     /gene="bglF"
                     /locus_tag="ACHURS_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFR1238733.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     NTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDVTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(2342..3178)
                     /gene="bglG"
                     /locus_tag="ACHURS_003066"
     CDS             complement(2342..3178)
                     /gene="bglG"
                     /locus_tag="ACHURS_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFR1238734.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKCAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
     gene            complement(3464..4189)
                     /gene="phoU"
                     /locus_tag="ACHURS_003067"
     CDS             complement(3464..4189)
                     /gene="phoU"
                     /locus_tag="ACHURS_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFR1238735.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(4204..4977)
                     /gene="pstB"
                     /locus_tag="ACHURS_003068"
     CDS             complement(4204..4977)
                     /gene="pstB"
                     /locus_tag="ACHURS_003068"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFR1238736.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(5068..5958)
                     /gene="pstA"
                     /locus_tag="ACHURS_003069"
     CDS             complement(5068..5958)
                     /gene="pstA"
                     /locus_tag="ACHURS_003069"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFR1238737.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(5958..6917)
                     /gene="pstC"
                     /locus_tag="ACHURS_003070"
     CDS             complement(5958..6917)
                     /gene="pstC"
                     /locus_tag="ACHURS_003070"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFR1238738.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(7004..8044)
                     /gene="pstS"
                     /locus_tag="ACHURS_003071"
     CDS             complement(7004..8044)
                     /gene="pstS"
                     /locus_tag="ACHURS_003071"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFR1238739.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKEIGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDETIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(8292..9365)
                     /gene="lpfD-O113"
                     /locus_tag="ACHURS_003072"
     CDS             complement(8292..9365)
                     /gene="lpfD-O113"
                     /locus_tag="ACHURS_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HFR1238740.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHRTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGGKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            complement(9376..11898)
                     /gene="lpfC-O113"
                     /locus_tag="ACHURS_003073"
     CDS             complement(9376..11898)
                     /gene="lpfC-O113"
                     /locus_tag="ACHURS_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFR1238741.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKRFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSCVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLLGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
     gene            complement(11923..12654)
                     /gene="lpfB-O113"
                     /locus_tag="ACHURS_003074"
     CDS             complement(11923..12654)
                     /gene="lpfB-O113"
                     /locus_tag="ACHURS_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
                     /protein_id="HFR1238742.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEK
                     ILRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRL
                     IPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPAN
                     SAGGQMTWRFINDYGAVSDPIKMTL"
     gene            complement(12702..13274)
                     /gene="lpfA-O113"
                     /locus_tag="ACHURS_003075"
     CDS             complement(12702..13274)
                     /gene="lpfA-O113"
                     /locus_tag="ACHURS_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HFR1238743.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSLVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT         3504 a         3399 c         3006 g         3425 t
ORIGIN      
        1 cgctaacccc gcttcattcg gttcggcttc gtcgccctga gggaaaattc gcgcccaggc
       61 aatggaaata cgcagacagg tgaagcccat ctcggcaaat aacgcgatat cttccgggta
      121 acggtgataa aaatcgatgg cgacatcttt gatattctct ttccccagga tgcgcggttc
      181 catttttccc ataacgccat gaggctgtaa atctgaggtc gtgctccctt tgccatcttc
      241 cagccaggca ccttccacct gattggcagc tgttgcgcca ccccaaagaa atgtttctgg
      301 aaatgctttc ataattaact ccttttatcg ttagcgaatg atggataaca gcggttcacc
      361 tgcgcttatc tgcgccgtgc cgtggggtaa tacgtctgta aaatcatcgc tattactgat
      421 taataccggc gtcgtcacat caaatccggc ctcgcgaata gcagggatat caaaagaaat
      481 cagccgatcg cctgtattga ccttgtcacc cacgttgacg tgagcggaaa agaatttgcc
      541 gtccagtttt acggtgtcga taccgacatg aatcaggatc tccacaccat catctgactc
      601 aatgccaatg gcgtgtaatg tggcgaacaa cgaagcaatt cgacccgcaa ccggagaacg
      661 cacttcacca accgagggca gaatggcgat acctttaccc aacaggccac tggcaaacgt
      721 ggtatcagcg acgtgaatga gcggcacaat ctctcccgtc atcggtgaac agataccgcc
      781 ctgctcaggt ggtgtaataa cctctggtgt tttctcttgc ggggcaccct gcgctggctg
      841 acgtttagcg gtgatgaaat gaagcatcac cgtaccgaca aatgcgcaac cgatggcaat
      901 gacaccgcca ataacgctgg cccagacggt gaaatcaatt cccgttgacg ggatggtttg
      961 catgaaggtg aaaatacttg gcaaaccaaa ggagtacact ttcgtttgcg cgtagccaat
     1021 gatggtggcc cccaaagccc cactgataca ggcgataaca aaggggtact tacgcggcag
     1081 attaacgcca tataccgctg gttcggtgat accaaacagg cccgtcaacg ccgctgatcc
     1141 cgccaccact tttttctgcg catcgcgttc gcagaggaag acgccgagcg ccgccccgac
     1201 ctgcgccata atggcgggca ttaacagcgg gatcatggtg tcgtagccca gcacggtgaa
     1261 gttattgata cacagcggca ccaggcccca gtgcagtccg aacatgacga agatttgcca
     1321 gaagccgccc attaccgcgc ccgcaaatgc aggaaccgcc tgataaagcc agagataacc
     1381 ggcggcaatc agttcgctta tccaggttga tagcggcccc accagcagaa aggtgacggg
     1441 tgtgataacc atcagacata gcaatggtgt gaagaaattt ttgattgccg acggtaacca
     1501 cgcattaagt cggcgttcca gaatgctgca caaccaggca gaaaaaataa tgggaataac
     1561 cgatgacgag taattcaaca atgtgaccgg aatacccagg aaatccagcc ccagcgcatc
     1621 cgctttttgc ccgttctcga aagcagtcag aattaatgga tgcactaacg ctccaccaat
     1681 caccatggca gtaaatggat taccgccgaa gcgtttcccc gcggtgtatc ccaggattat
     1741 cgggaagaac caaaacaagg catcactggc gctgaataaa attaaataag taccactttg
     1801 ttcggtcgtc cactgaaaag tgagcgccag agccagcata cctttcagga tcccggttgc
     1861 cgccatcaaa ccgatcagag gcgtaaaaat acctgaaata acataaacaa agcggtttag
     1921 cagattacct ttctcatcat tttccggtgc ctgttgcgct ttttcgccaa ggcctgccac
     1981 gctgttgacc gccaggaaga catcggccac atggttacct atgaccacct gaaactggcc
     2041 accgctttcc accaccataa taataccggg ggtgtttttc agtacctcag cttgcgcttt
     2101 gctttcatcc tttaacttaa aacgtaatcg cgttgcgcaa tgcatcagac taacaatgtt
     2161 atctgcgccc ccgactcctg cgactatttt tctggctaac tccgtcataa cttgccctct
     2221 accgctttgc ggcaaaactc ccaaaaaaaa actgaaaaaa acggcctgac gtgaatcaag
     2281 caattttttt caggttttgc ccgcttagtg cggtaacaat cctttactca gtaataatat
     2341 ttcagtgttc tttgcgcacg cgctctatat ttatggctaa aaacataatc tctgcgggtg
     2401 aaattttacg ttgatactgc aaaccaataa aaatggcgat ccgttccgca cattgccatg
     2461 cttgcgggta attttgtttt actgcttgtt gtaatgattc atcactatcg ttaattgaag
     2521 catgttcaag aatacgccag gataaaaact tcagatgtgt aaccagtcgc tgataactca
     2581 agctttcttc ctggtaatta aggctgaact gaaattttat taattgcagc atttcgcgca
     2641 ttaactgcgt gacacctgca acatcctcca tatttccgct catttgggca ctgaccagat
     2701 gcatggcaat aaagcccact tcatctttcg gtaactgcac gcccaaccgt ttatcaataa
     2761 tggttaacgc ttcttcccct agctggaact ctttcgggta aagccgctgg atatcccaca
     2821 gcaacgggtt gggcagcaac acgttttgct gaaagcgttt aatcgcaaac tggcaatggt
     2881 cagttagcga gatataaata ctgtcctgta attttcccaa gcgctcctgc gctaaagaga
     2941 taatacgatc acaggttgcc atcacctcaa gaggaatatg acttaagagt tcgcttaatc
     3001 gcccgttcag ttcatgactg ctcaaggcat actctttttc tattccactt gagttaattc
     3061 tttcgccagc gcatttttga aagccaattc ctcgccccat gacgactttt tcccgctgtt
     3121 gatcatcaat aaccaccaca acattattgt tgagaatttt ggtgatttgc atgttcatag
     3181 caaggacctt ttttataaac aaaaaaaacc gacttcacca gtattctctg gttatgtcag
     3241 gttttgcctg cgaatgcagt aacaatccag tcatttatta atggttttta taacgaacat
     3301 ccaggttcgg aaattaattt aattgcgtgc agagaaaata gcaatgcgct attgataaaa
     3361 atatgaccat gctcgcagtt attaactttg tgtaatttta ggaatttata aagttatata
     3421 taacaaatcc caataattaa gttattggga tttgtctggt gaattatttg tcgctatctt
     3481 ttcccgccag cagtttatcc agctcatcac cgcctacatg acggaaatcc tgccccttca
     3541 cgtagtagaa gataaactca caaatattct ggcagcggtc gccaatacgt tcgatagaac
     3601 gcgcgcagaa cagcgcagta agtacgctcg gaatggtacg cgaatcttcc atcatgtagg
     3661 tcatcagttg acgaacaata ccttcgtatt cctgatcgac ttttttatct tcacgataaa
     3721 tacgtaccgc ttcgtcaatg tccatccgcg cgaacgcgtc cagcacgtcg tgcagcatct
     3781 ggatggtatg acggcccagc gactccagac ttaccagcaa cggctgatgc tgctgggaga
     3841 atttctccag cgcagtacgg cagattttgt ccgccacgtc gccaatacgc tccagctcgg
     3901 caatggtttt actgatcacc ataaccagtc gcaggtcgct cgccgtcggc tgacgtttgg
     3961 cgataatgcg cacgcaggct tcatcgatcg ccacttccat catgttgacg ttcttgtcgc
     4021 cttcgatcac gcgcttcgcc agatcgctgt cctggttatg catcgcggtg attgcatcag
     4081 aaagctgctg ctccaccatg ccgcccatgg tcatcacctg cgtgcggata ctttccagtt
     4141 cggcgttgaa ctggccggaa atatgtttat taagattgag actgtccata acgcactcct
     4201 gaatcaaccg taacgaccgg tgatgtagtc ttctgtttgt ttcttcgctg gcttggtgaa
     4261 cagatcgtcc gtgttgctga actcaatcaa ttcgcccagg tacataaacg ccgtgtggtc
     4321 ggaacaacgc gcagcctgct gcatgttgtg ggtaacgatc accacggtgt aatcctgctt
     4381 cagttcggtg atcagctctt caatacgccc ggtagagatg gggtcgagcg ccgaacacgg
     4441 ttcgtcgagc agcagcactt ccgggcgaat ggcgatacca cgcgcaatac acagacgctg
     4501 ttgctgacca ccagagagag agtaaccgct ctggtgcaat ttatctttgg tttcgttcca
     4561 caatgcggct ttggtcaatg cccactgcac gcgctcgtcc atgtcggcac gggagagctt
     4621 ctcaaacaga cgaacaccaa aagcgatgtt gtcgtagatg gacatcggga acggcgtcgg
     4681 tttctggaac accatgccca ctttcgcacg cagcagtgcg atatcctgag agttggtcag
     4741 gatgttgtcg ccatcaagca gaatttcacc ttctgcgcgc tgctccggat acagttcaaa
     4801 cattttgttg aaggtacgca gcagcgtcga tttaccgcag ccggacggcc cgataaacgc
     4861 cgttacctgg tttttagcga tatccaggtt gatgtttttc agggcatgga atttgccgta
     4921 gtagaagttc aaattacgaa cctgaatttt actcggggca gtttcaacca tactcattgc
     4981 aatcttttcc tcatcgtacc tgatgctttt cgctaatcag gcttaccgta ggccgggtaa
     5041 ggcgttcacg ccgcatccgg caaagtgtta accgtgttta ttcttcgcaa aaacaacgcg
     5101 cgccagaatg ttcagcagca ggacgcacag ggtaatgatc aataccccgg cccaggccag
     5161 ttgttgccat tccgcaaacg ggctcatcgc aaacttaaag atcgtcaccg gcaggttggc
     5221 gatcggctgc atcatgtccg tgctccagaa ctggttggag agcgcggtaa acagcagcgg
     5281 cgcggtttca ccggcaatac gggcaatcgc cagcaggata ccggtcataa tcccggacac
     5341 cgacgctttc agcgtaatcg cggagatcat cttccacttc ggtgtaccca gcgcataagc
     5401 cgcttcacgc aggctgtccg gcaccagttt cagcatgttc tcggtggtgc ggataacgat
     5461 cggcacctgc aacagcgcca gcgcaattac gcccgcccag ccggagaagt gctccatctg
     5521 tgccaccacg atggtgtaga caaacagacc aaccacaatc gacggcgcgg agagcagaat
     5581 gtcgttaatg aagcgaatca cttctgccag ccaggattta cgaccatatt ccgccagata
     5641 aatccccgcc ataatgccca gcggcgtacc gaataccgtg gcccacaaaa ttaacagccc
     5701 gctacccgcc agggcgttcg ccagaccacc accttccgta ttgggcggcg gcgtcatttc
     5761 agtgaacagc gccagcgaca taccgtcgat accgcgagtg atggtggaca ttaaaatcca
     5821 gatcagccag aacaggccga aggccatcgt cgccatcgag agcgtcagcg caatacggtt
     5881 tttgaggcgg cgacgcgcct gcattttgcg gcgagattca gccagcgccg cagtggtttg
     5941 catttcaacc atagccatta gcgtgccccc tcattcttag ccaggcgcat aatcataaac
     6001 ttcgatgcgg cgaggacgat gaaggtaatc acaaacagga tcaggcccag ttccatcagt
     6061 gcggcaacgt gcagaccgga ttccgcttcc gcaaattcgt tcgccaacgc agaggtgata
     6121 ctattgcccg gcatgtacag cgaggcgctg tcgagctggt aggtgttacc gatgataaag
     6181 gtcaccgcca tggtttcacc gagcgcgcgg cccagcccca gcatgatgcc gccgataaca
     6241 ccatttttgg tgaacggaag aacgatacgc cagataactt cccaggtggt gcagccaata
     6301 ccgtaggccg actctttcat catcaccggg gtttgttcga acacatcacg cattaccgcc
     6361 gcaatgtacg gaataatcat gatggcgagg atcacgcctg ccgcgaggat accgatacca
     6421 aatgcggggc cagagaacag cgcgccaaca atcgggatat tcgacatgat attgccgacc
     6481 ggctcctgaa agtaaacggc gaacagcggc gcaaagataa acaggcccca catgccgtaa
     6541 acgatacttg gaatggctgc cagcagctca atggcgatac ccagcgggcg tttcagccag
     6601 ccaggcgcaa gctcagtcag gaacagggcg ataccgaaac tcaccgggac ggcgatcagc
     6661 agcgcgataa acgaagtcac caacgtaccg tagatcggca ccagcgcccc gtagatatcg
     6721 ttcggtgcat cccactcttt ggtccatagg aaagccagac caaatttctg aatgctcggc
     6781 caggaggaga tgatcagaga gacaataatg ccacccaaca tcaatagcac aatcagcgcc
     6841 gccagtttta ccagcacgct gaaaattatg tcgccctttt tacccggtgg gttaaaagca
     6901 ggcttggttg cagccataag ttactcttca gttaaacgcg tttacaaagt cattttgtaa
     6961 tgccggatgc ggcgtaaaac accgtacccg gcctagagtt ttattagtac agcggcttac
     7021 cgctactgtc tttaatattg gtcttccacg cagcgcgaac ctgttcaact acactatccg
     7081 gcaggctggc gtaatccagg tcgttcgcct gtttagcccc ggttttgtac gcccagtcga
     7141 agaatttcag cacttctgtg ccttgttctg gtttcttctg atctttgtgg atcagaatga
     7201 acgtggtaga ggtaataggc catgcatctt cgcctttctg gttggtcaga tcctgagcga
     7261 aggttttgct ccagtctgca ccttttgctg cattagcgaa gttttcttcg gtcggactaa
     7321 ccggtttacc atcagcggag atcagtttgg tgtacgccag gttgttctgc ttcgcgtaag
     7381 catattcaac ataaccaatt gcacccggca gacgctgaac gaacgcggcg ataccgtcgt
     7441 tacctttacc gcccagaccg atcggccatt ttacggtaga gccagtacca acgttgtttt
     7501 tccactcttc gttcactttc gccaggtagc tggtgaagac gaaggaagtc ccggagccat
     7561 ctgcgcggcg tactacagca atgttttgtg aaggcagttt cagacccgga ttcagtttgg
     7621 cgatggtttc atcatcccac ttcttgattt tgcccaggta gatgtcgccg agggttttac
     7681 catccagcac cagttcgcca gacttcagcc ctggaatgtt aaccgccagc accacgccgc
     7741 caatcacggt cgggaactgg aacagacctt cctgagccag tttttcgtca gacagcggcg
     7801 cgtcagaggc accaaaatca acggtattag cgataatctg ttttacgcca ccggaagaac
     7861 cgataccctg gtagttaact ttattaccga tttctttctg gtaagtgtca gcccatttgg
     7921 catacaccgg cgcagggaag gttgcacctg cacctgtcag gcttgcttct gcaaacacag
     7981 agaaagcgct catcgataag gtcgcggcga caacagttgc gacggtggta cgcataactt
     8041 tcataatgtc tcctgggagg attcataaag cattgtttgt tggctacgag aagcaaaata
     8101 ggacaaacag gtgacagtta tatgtaagga atatgacatt tttatgacag aaaggaatta
     8161 acaaataact tactgatttt aaatgtgaag atgaaaatcc cacatgtcat ctttttgtca
     8221 tgatgcattt aaaagtgata aagaaatggg tgagatgaca acgatgtgat gaacaatcgt
     8281 tgtcagacct attagcgcac aatctcaaca gttatcgtcg ctgttgcagt aaattgcccc
     8341 ggggctgggc gagctccagt aaggctcgca ggagcagctg aaaaagttac actacctgat
     8401 ttttcaccat acactgttga tttacccatg tctgttggca actcaccacc gttcactttg
     8461 atttccttat tatttttgtc gtaaattttt atccccacgt tagagtttga agttacgacc
     8521 attgaattgt ccgtattggg ttcggtagca taaaaagacg tactgatatc atcggtattc
     8581 agtatcccca ggttttcaca ctcaatagtg actgttttag taattttatg tgttgttacc
     8641 gcacttccaa caacagatga aaaatctgca gctggtattt ctttaaaatc aaaataaact
     8701 ggctgtaaat tatttatttc gcaactttgc ggagctgtaa tatcgccccg aatataaaca
     8761 tcagccatcg gctgtgagta atccaccggg gtgtcacggc taattgttcc atacaatgtc
     8821 actatctttg tttcaggaat aatcaatttt cctgcaaatg tcttcttgat ataaaaatat
     8881 actatcgctt tacccccact ggatatactt aaaggttcaa tgcggttgca attgtacttt
     8941 gtattaccag acgggttgtt agcatgatat tcaaaaggtg cattcacata cccaacccca
     9001 agaatatcta tagcaatccc aacatcaagg ttcggattaa gatcataata attaatatta
     9061 gagtgaaccc cagcataagt taatgcctgg tttatttttg acgtataata gatatagggg
     9121 aattctccta cattaccagt atcacaatga cagcgtccag gatatgagga tccattagtt
     9181 acagtggtat taaatgtcgt tccaacctta ttttcgttcg cggtaataag atatcccgag
     9241 aaatcaagcg tacggtgaaa tacgccatta tcaggggtac agataccttt aggagctgca
     9301 agtgctacac tacttaacga gagcataaac acagcaaagc accactgttt gatatacttg
     9361 ttcataaaaa tccttttaat gacatgtcac accctgttca attaatccgc tctcactttc
     9421 tggtgtcgaa agtgaataat tgaagatgca ttttgttgag gcgttttctc cccaggataa
     9481 cgtaacctgt cctttttctg gtagaccaga aagataaagt tcgccacggt caccgacaat
     9541 tcctgaatct gccaaacttg catcttgtat cgcagcaatg gctccaaaag gtaccggttt
     9601 accatttgca tttaacactc tgaagagaac gcgataaccc acatgagtca caaactccgc
     9661 tttaaccact gcaccacgcg tgggaaccat cgtaacgacg gtattatcaa gttctatatt
     9721 cgacttcgag aaatagttat cgctgattgc tacccgattt tcatcataag ggtttagctg
     9781 tgtcttgacc gtatagccgc gccagtccgt cgcagcaccg cgcatattat ctatttctaa
     9841 cccggcggcc ccaggtgctt taacaagagc aatagtttca cctaactcct gacttaacgt
     9901 taccccttcg ctgtgtgcca gaattccccc tctgatacca taattcagcc tctgggaatt
     9961 ttggctgtag ttatagctgc cgttcaccgt gccataagag ccatcatagg tcagggacat
    10021 attaccgcca acctcatcat catgactgtt ataggactgc attaaattcc atgaaaggtt
    10081 atttctttcc agcgccgtac cgctgagtcc gagttgatgt gagctaccgg aattatcgtt
    10141 ttgcgaaaca gaataggtgg cgtaactatt accccaccag cccaataaag gaacagataa
    10201 agagagcatg tagatctgct cactggtgtt atcactggca tggttgttcc ccgtcccctg
    10261 tctggcagta ttggaataac tccaggagac gttccacgag agacgcttcc agctactgct
    10321 gtagccaaat tgcattaatc gttcgacatc atcggtatgc cagtattgtt gccggcttaa
    10381 tgtcaggtat aaattgccat aatctctccc caacgactgc gttagcgtcc cctccacgca
    10441 actacgtcgg ttacctgttt cccaaaagtc ttcagggctg ttgcgccgcg atgcccactc
    10501 attgagggta tagtagccct cggtggagta acgatagcca accaagcgca agctagtgtc
    10561 tgtgtcgtca aatcgttttg aatagagaaa gcgatatgac tgacccgttt ctgtatcatc
    10621 atggctaaaa ttcgcacgag catgtgtcac atcgaaagaa attgcgccca aagcgccaag
    10681 atctttaccg acacccagag ctgcagaacc ataattttca gcagcctgca tacctccata
    10741 taaagtgatc ccgtgggaaa aaccatgaag tatttgcgcc tgaaggacgt ccggtgtaaa
    10801 tccgtcttca tcgcgcaatt cacccacgct gacatcaaca tctgtctgac cttcacgttt
    10861 aagaatcgct aatgaagcat aaggttgggt gaatcgttgt tcgctaccgt cttcttcttt
    10921 gatggttacg tccaaatcgc cacctgtact ggcggtgttg agatcggtga tctcaaaagc
    10981 accttgtgga acggcagact gataaatcac gtagccattc tgccgaatga tgacggtggc
    11041 gttggacttt gcaatcccac gaaccacagg agcaaagcca ttctggctgg cgggtagcat
    11101 atcgttatca gtatacaaac gcgcgccgcg aatttgcgta ctgtcgaaaa tatcgctcgc
    11161 cgtccacgta tcaccaatca taatctggct tcttaacgcg gcaatatcgc gctgtatcca
    11221 ggtactaatg gagttgtatt ccgcacggcc atcgctggtg tttaaagtac tgtaattacg
    11281 tagccgccac ggtccaatat tcagtccatt gcgcagattg agataactgg tatctgtctc
    11341 ttgtgaacca taatctgaac gtagtgtatg tgaaccagaa aaatcgtagt tgatggtgag
    11401 cgcgttaatc ccctcatccc aacgcgtggg gctgatgtaa tcataagcca tcgcgtccat
    11461 gtagatttgg ggaatggata tattcagctg caatttatca ctgtcaaact gccaggatga
    11521 gccttcaata cgtggagcag gatcgaggca aaccgtctca tccacatcat tgaacgattt
    11581 gagcgcatca acttttatgc caaaggagga cagtgttttt ttaatggcaa taaagcaagc
    11641 gtgcagatca gcatctggat tatcctgaac aaatgtcaca tcctgacgtt caaacatttt
    11701 accgttgata aaaatatcaa cgctataaac acctgggggc atcaggtttg cccgattaaa
    11761 ctggcgaata tcgactccgg attgatggtc gatttccaga agcgccggat tgaatacact
    11821 gacctcggcc atcaggtttt gcgagaaaat cagacacgtc cctgccgtga ggccaaccac
    11881 tattctggtc gtcatcataa tacgcctatc cttatccctt gcttacagcg tcatttttat
    11941 cgggtcactg actgcgccgt aatcattaat aaatcgccag gtcatttggc ctcccgcaga
    12001 attagcaggt aaaggtaacg tgagcgtttc aaaaggacgg atcatattga ggggcacatc
    12061 atggccatca acgatgacat ccatcacact gatatgaatt gggttgggat tctctgctat
    12121 cagatttcgc ccttcccggc gccatttcac tttcggcgca gcatcttcgg gaataaggcg
    12181 gatatttgct ggtcgccaga acagcttgat acgcgttttt actgctattt ccagcgagtt
    12241 cgtcgctttg ttgctcgctg atggcggaat atttttaata ttcagccaga agactgactc
    12301 gcgatcatca ggcaatgaca cacctttggt gtgaataatg cgtaatattt tttcttgcga
    12361 aggttctaat cgtgatactg gcggaataac ggtaaacggc gctttgcttt tatcctcttt
    12421 attaaatgga tctacccaag cctggatgag ataaggtatt ttttcttctt tgctaaatac
    12481 agaaatagat ttatcagcat tattacttaa gtaaaccacc cgcgtaccac caatgacaac
    12541 gcctgcataa gttaatgctg gttggcctaa aaaaacgctg agtataatcg cggtaaataa
    12601 cttacatgaa actgttgttt taaagagcct gattaaacaa ctttttctgg acataacgac
    12661 tccctggcca aagccaactg aataaaaagg cccttgataa attatttctt atattcgaca
    12721 gtaaattgtg aatcagcgtt cgccgtacca gccgttacgg tcgcattggt tgccacatag
    12781 cgtgcaataa acttcatttc agccttgcct tcagcatcga tagcggtata agcagagtcg
    12841 ttataaagtt taaccagcga attatcggac tggttaaaaa tacgaatacc aacgccggtt
    12901 gccgcaatcg cttgtcctgt accggtaaaa tcaccagggt tgcctgctgt aagcggcgta
    12961 cccactttca ggtcgccgtc gccgccaggc gcttctgtac catcaaaacg aacggctgct
    13021 tgagtgtacg ttgccgggca attttcaagt ttaatactga acttctgtgg cgacgccaga
    13081 ctacctacac ctgaaaatgc atttttactg actttaccca gtgggacaat ttgatcggct
    13141 gtatcggagg taacttcaca agtagtgtcg acaatttcac cgacgaagtg aacaacacca
    13201 tcttctgcca gtgcatgccc ggccagcatt agagatgcca gagtgaatgc accgcctata
    13261 atattacgct tcataaaaac tccattgata taatatatcc tttaccatga atcatcactt
    13321 atattaataa gtgt
//
LOCUS       DAZPDU010000120        13262 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZPDU010000120 DAZPDU010000000
VERSION     DAZPDU010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13262
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.121"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            210..422
                     /gene="cspG"
                     /locus_tag="ACHURS_003076"
     CDS             210..422
                     /gene="cspG"
                     /locus_tag="ACHURS_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFR1238744.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            433..621
                     /locus_tag="ACHURS_003077"
     CDS             433..621
                     /locus_tag="ACHURS_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFR1238745.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            596..826
                     /gene="ymcE"
                     /locus_tag="ACHURS_003078"
     CDS             596..826
                     /gene="ymcE"
                     /locus_tag="ACHURS_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFR1238746.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            816..989
                     /locus_tag="ACHURS_003079"
     CDS             816..989
                     /locus_tag="ACHURS_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFR1238747.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(1038..2111)
                     /locus_tag="ACHURS_003080"
                     /pseudo
     CDS             complement(1038..2111)
                     /locus_tag="ACHURS_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            complement(2183..4927)
                     /gene="torS"
                     /locus_tag="ACHURS_003081"
     CDS             complement(2183..4927)
                     /gene="torS"
                     /locus_tag="ACHURS_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFR1238748.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFATQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHQEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     QLMGAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            5010..6038
                     /gene="torT"
                     /locus_tag="ACHURS_003082"
     CDS             5010..6038
                     /gene="torT"
                     /locus_tag="ACHURS_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFR1238749.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASMPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(6011..6703)
                     /gene="torR"
                     /locus_tag="ACHURS_003083"
     CDS             complement(6011..6703)
                     /gene="torR"
                     /locus_tag="ACHURS_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFR1238750.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6823..7995
                     /gene="torC"
                     /locus_tag="ACHURS_003084"
     CDS             6823..7995
                     /gene="torC"
                     /locus_tag="ACHURS_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFR1238751.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNESDTAGKAHGDKKDEK"
     gene            7995..10406
                     /gene="torA"
                     /locus_tag="ACHURS_003085"
                     /pseudo
     CDS             7995..10406
                     /gene="torA"
                     /locus_tag="ACHURS_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            10407..10952
                     /gene="torD"
                     /locus_tag="ACHURS_003086"
     CDS             10407..10952
                     /gene="torD"
                     /locus_tag="ACHURS_003086"
                     /inference="COORDINATES: protein motif:HMM:NF003442.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFR1238752.1"
                     /translation="MFSRELDDEQLTQISSAQMAEWFSLLKSEPPLTAAVNALENRIA
                     ALTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKRLLVEAGMETSGNFN
                     ESADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTALRQWLPEFAARCRQYDSF
                     GFYAALSQLLLVLVECDHQNR"
     gene            complement(11045..11350)
                     /gene="cbpM"
                     /locus_tag="ACHURS_003087"
     CDS             complement(11045..11350)
                     /gene="cbpM"
                     /locus_tag="ACHURS_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFR1238753.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(11350..12270)
                     /gene="cbpA"
                     /locus_tag="ACHURS_003088"
     CDS             complement(11350..12270)
                     /gene="cbpA"
                     /locus_tag="ACHURS_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFR1238754.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNHQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            12531..13262
                     /locus_tag="ACHURS_003089"
                     /pseudo
     CDS             12531..13262
                     /locus_tag="ACHURS_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3287 a         3303 c         3427 g         3245 t
ORIGIN      
        1 ggacggctaa aataaaattt gcttaatctc aattatcatg cgttaatagc tgcgtcggtt
       61 tgaaagacag acagcataca aagtagttta ctaaagcagt tctcattatc aggcattatc
      121 cccttctttt gagtctctct cctgaacact aagtagtttc tgtattaaag ccctgtttgc
      181 cgaaaggccc aaaatgaagg aagtaaaata tgtctaataa aatgactggt ttagtaaaat
      241 ggtttaacgc agataaaggt tttggcttta tcactcctga tgatggcagc aaagacgttt
      301 tcgtccattt caccgccatc cagagcaatg aattccgcac gctgaacgaa aatcagaaag
      361 ttgaattttc tattgagcag gggcaacgtg gccccgcggc agcgaacgtt gttacgctct
      421 aaggttgccg ttattactca acatctccat ttccgctgtc catgttgtca tggttcacag
      481 taccgcacat cggcattcga tgtgacggag cgaaaccctt tgggcgctaa gtgtattttt
      541 tgtaaatcga cgatgatcac ctttgataac gtcgcgctgc aaatacgcac tgaccatgcg
      601 ccgctggatt tcacaaaata atatcaggct ccctcgtgga gcctttttta tatctgcctt
      661 atttttcttc aacgctgtat gtatagtaag cgataacctg ttgattattg aatctttcgg
      721 ggagatggct tataacattt cttacctgac cagggtaccg ggaaccaaca ccttactggc
      781 gtgttgctgt cttttaagac cagaagaggt taacagtgaa tattgaagag ttaaaaaaac
      841 aagccgaaac ggaaatcgcc gactttatcg cgcaaaaaat cgccgagctg aacaagaata
      901 cagggaaaga agtctctgaa attcgcttca ccgcacgaga aaaaatgacc gggcttgaaa
      961 gttatgatgt caaaatcaaa ataatgtgat tttgtgaaca tcaccccgtg cgaggtgatg
     1021 ttccgcttgt tgctaattta gtgaccaatc attggcgctt gtggaattaa gcgtcggtac
     1081 aattcctccg gcaccgggct ttgccatact cccgcataca ttgcgtaacc aatcaccgcg
     1141 aacataatcc tcagaaccag tagcgtcatt aaccagccag acaacgcaaa agctttttta
     1201 tttgccgcag gtttttgcag tgaaaaggtc aatgttgagg ctaccggaca tgactctacg
     1261 caagtcatac agccggtaca ttccactgtt cgtacctgaa ttaatttatc taccgggatc
     1321 cgtgatgggc aattttttgc gcatttgcca cagccgatac aactttcggc attgcgacga
     1381 atcttaaacg gcgataatag cgaaaccacg cccatcagcg cgccatatgg gcaaagataa
     1441 cgacactagg catggcgaat aaacaggctg gcaatcagca aaacggtcac gctgattaat
     1501 gtcgtggtcc ccatatgacg aaagaaatcg agcattttta cgtccatcac cacgctgtag
     1561 ggcgacaaca taaaatagtg aatcgcctga gcgggcatca ataacgcgat atagagaaaa
     1621 aaactcaaca gcaaatactt cacgccgcgc agaggaatat ccagccagcg gggaaggaca
     1681 cattgccgac caaacagttt gttaccgaga tcgccgatta attcagaaag cgtaccaacc
     1741 gggcataacc atgagcaaaa ggcctttttg agtaatagac tgatgacgat aaaagcgacc
     1801 aataacagca tcgcggcggc gtggacggac ggtaactgac ctgttgtaag gctatatttc
     1861 agattcatca gaccggcaat cggtagccag ccttcgatac ctcccggtct ggcgacaaat
     1921 gtcgtgctac ttgccgtttc gtaatagcgc acccaatacc agaacgtgat ggcaatataa
     1981 atattcattg ccaacagtaa taattgcgtc gctttacgcc aggtcgtggc attgcgccag
     2041 tcattccacg gtaatttgcc gcccgtcgtg cctggccgcc gctgccagcg ggttcttttt
     2101 ttctctgcca tgattttgcc agtccgttaa gttgcatacc aaatgccact attctagttg
     2161 ttcttaactg gctgatattg attcaaatcg cgttcaggtc tttcttatgc aaccatgctt
     2221 ccagagcggc aacactgcgt gtaatttctt cgtgtggaag gggggcagat aatggctgct
     2281 gctccagttg tgcgcatagc tggctggcga tacgcattcc caaactcgag caactgcttt
     2341 ttagctgatg cgcggcacgc tttattttct cgctatcctg actggcgcgg gcaatgtcga
     2401 tttcatcgag aagcggcagg gcatgttgtg taaataatac cagccattcg tggatcttct
     2461 ccgtccccat taactgagca tcttcattga gttgcgatac atccagcgat tgatcgttat
     2521 tgacttgcag ttggagatag tgcgccagta actgaccgag cacttcacgc ggcaccggtt
     2581 tagggataat cccgcggaat aatgaactgg tacgctggcg cagcgtttcg tcaatgacat
     2641 gggcgctaaa gccaatcaaa accagcgacg gatattgctg tgccagttgt cgggcaagcg
     2701 taatgccgtc gacatccggc agatcaaaat ccaccagtgc ggcagcaaac ggttcgctat
     2761 tttgcagtgt ctctaaagcc tgcgcggcat tgccaacagc aacaacctgc gcaccactgg
     2821 tgttcagcat ctcaacggta attcgctggg ttagcgggtt atcttcaatt aacagcaaac
     2881 gtaaaccgtc aagacgcacc gcctgattga ctgtttttgg cacgggtgcc gtggcaacac
     2941 gtaacggcaa gcgtaaacaa aaacagcttc caacctccgg cgtgctggtg gcgctcagtt
     3001 cgccgcccat cgcctgggcc agacggctac tgatagtcag ccccagcccg gtgccgccgc
     3061 gtttgccgct tacctgcaca aatggctgga agatttctgc cagtttcgcg ggatcaatac
     3121 cgcagccgct gtcttccact tcgaccagcc attgctcgcc atcagtgcga ctacgcagga
     3181 taatgtaccc ttcgtcagta aaacgcaggg cgttgctcaa caggttggtt ataacctgac
     3241 gaatacgtcg tggatcgccc attaacgcgc acggcatatc atcggcaatt gccgttgcca
     3301 ggcgaatcgg gcgacctttc acccgtccgc tcattaattg cagggtactt tccagcagcg
     3361 ggcgcggttc aaagggctca tcgctgaccg aaacattctt gccacctgct tcgatagcgg
     3421 aataatcgag aatatcgttg aggatggtca gcaacgattc accagagtca gtaattgccc
     3481 gcaaatcatc acgctgggcg ttaagtgcgg ggttatctgc cagtagctgt gcggtgccga
     3541 gaataccgta cagcggtgtg cggatctcat ggctcatcgc cgccagaaac gccgattttg
     3601 cctggctggc tttttctgct tccgcccgtg cctgtcggtg ttctatcact agttcctgca
     3661 attcagctgt acgcgctttg acctgcgccg ccagctgttc acggtggcga ttcagtgcat
     3721 gaacactgct gcgaaacgca cccatcagct gcccgatggt gtccagctcc cgtacgccag
     3781 cggtttccgg gaaaggggag tcaatatcac cgtccagcag ccgttgcagc gcctgcgttt
     3841 gttcggcaag tggacgcgtg actgagcgat aaaccacgcg ccagaggatc agaatcagtg
     3901 cgcaaagtga aaccatcccc agcaataaca ggctgtattg cccgcgtgca ctggcttttt
     3961 cctgatgcgc cagtccgtgc tgattacgca gctcaatggt gtcgaccagc tgactgactt
     4021 cgctactaaa ctgcgcgaac tgggcgatat tattttgggc gagagtttgc aggtgattgc
     4081 tgatttcact gtcctgctgg aacagcgcca acagatcgct atattggcta acggtagtta
     4141 acgttgttgc gacctgcgca cgaactcccg gatcttcaat gcgtatttgc cgacgttgca
     4201 gaattttcac cgcattattg agctgctttt ccagcgttgg tgcatttttc tggatctgct
     4261 ccagccccag attcatcacc atttgctgca cccgcagagc gctaaggcgc agttcattca
     4321 tctggttaac atactcaaga tcgatatcaa tcagccgatc gagtgcactt tcagcagcct
     4381 gacgctgatc ttgttcgatc aaatcgtaaa tcccggcctg ggtcgctcca gcggaagttg
     4441 tcgcattatt cgcctgacct tgcgccaggc gtgcgatctc atcggcggca gcgactatct
     4501 gctgactgag ttgctgttgt tgctggcgta gttgcagacg ccgccccacc agttcccctt
     4561 gctgacgtaa cgaacgggag atctcctgct cctgttgttc aatagcggtg gtatcaaaac
     4621 cttgttcccg taacgcttgc agcaacgcat taatcttcag gctttgtgcg gtgagcattc
     4681 gcccctgcgc ctgccacatc ttttcgttat cggcactggt caggttctgc gtggcgaaaa
     4741 gttcccaggc gctggcttcg ctcaactggc gcgccatatt catggtagga atcaatgcct
     4801 gagtattgtc tttttccacc tggctgataa agcgcaggtt gtaccatccc accagggtac
     4861 tggtcagggt taacagcgcc atcagggcaa agcccatcca gagtcttcgg gtcagggtta
     4921 aattcacggt cggtgcactt taggtgaaaa atgttgagtc gcaaagcgga atgcatctag
     4981 cataaagcct tattattgat gaggctatca tgcgcgtact gctattttta cttctttccc
     5041 ttttcatgtt gccggcattt tcggctgata acctgttgcg ctggcatgat gcgcagcatt
     5101 tcacggtgca agcctctatg ccgcttaaag ccaaacgcgc atggaaactg tgcgcgcttt
     5161 atcccagcct gaaagattca tactggttat cgttgaacta tggtatgcag gaggcagctc
     5221 gccgttatgg tgtggattta aaagtgctgg aggcaggcgg ctacagccag ttggctaccc
     5281 agcaagcaca aatcgaccag tgtaaacagt ggggcgcaga ggccatttta ctcggtagta
     5341 gcacgacctc atttcccgat ctgcaaaagc aggtagcaag tctgccggtg atcgaactgg
     5401 taaatgctat tgatgctccc caggtgaaaa gccgcgttgg tgtaccctgg tttcagatgg
     5461 gctatcaacc tgggcgatat ttggtgcaat gggcgcacgg taaaccactg aacgtgctgt
     5521 tgatgcccgg acccgataat gccgggggca gtaaagagat ggtcgagggt tttcgcgcag
     5581 ccattgccgg aagcccggtg cgtattgttg atattgcgct tggtgataac gatattgaaa
     5641 tccagcgtaa cctgttgcag gagatgctgg aacgccaccc agaaatcgac gtcgttgccg
     5701 gaacggccat tgcggcagag gcggcaatgg gtgaagggcg taacctgaaa acgccgctta
     5761 ccgtggtgtc gttttatctt tcacatcagg tgtatcgcgg gctgaagcgg ggaagagtga
     5821 ttatggctgc cagcgatcaa atggtctggc agggggaact ggcggttgag caggccatca
     5881 ggcaattaca ggggcaatcg gtgtctgata atgtcagccc accgatttta gttctgacgc
     5941 cgaaaaatgc cgaccgtgaa catattcgcc gctcgctgtc accaggggga tttcgtccgg
     6001 tctattttta tcagcacaca tcagcggcta agaaataacc ttcaccatgt tgcgtcacca
     6061 gtaaatccgc gctgagttta tgacgtaaac gacgaattaa cacatcgacg gtgcgcaggt
     6121 cagggttttc cacccgacgc gcagaaagca tacgtagcag acgttcacgg ctgagaattt
     6181 cgcccggatt cgtcacaaat gccaccaaca tttcatactc tgcgcgggtc agtttaatcg
     6241 gctcgccatc ccgctccagc gtatggcgcg acacattcag gcaataaccg gcaaagcgat
     6301 agcagttgtc ctgagtgtgc ggttgagctt gtcgcgcgag gtcgattcgc cagagcagat
     6361 ttttcacccg tactaccagt tcgcgcagtt ccagcggttt ggtgacgtaa tcgtctgcgc
     6421 ccatttccag cccaacaata cggtcaatcc gatcgctgcg tccggtaacc agaataatcc
     6481 ccaccgttga gcgttctcgc agggcgcggg ttaacatcag gccattttca tcgggtaagt
     6541 tgatatccag cagaattaaa tctaccggct gattctgcat aatttcccgc agcccggcac
     6601 cgctcgctgt aacggaaacg gtatacccct cctgagtgaa gtaagattgt aatcgcgcct
     6661 gggtaaccgg ctcatcttca acaataacaa tgtgatgtgg catcagaggg ttttactcat
     6721 tctgttcata ttctgccgta agccgttcat cctgaccagt gccgctgttc atatttgctc
     6781 attaagatcg cttcactaaa ccataatttt acaggggcta ttatgcggaa actctggaac
     6841 gcgctacgcc gacccagtgc tcgttggtcg gtactggcgc tggtcgctat tgggattgtg
     6901 attggcattg cgctgattgt attgccacac gttgggatca aagtcaccag cacaaccgaa
     6961 ttttgtgtca gttgccacag tatgcaaccg gtgtatgaag aatataaaca gtcggtgcat
     7021 tttcagaacg cctctggcgt gcgagctgaa tgccatgact gtcatatccc gccggatatt
     7081 ccaggcatgg tgaagcgcaa actggaagcg agcaacgaca tttaccagac ctttattgcc
     7141 cactccattg atacacctga aaaattcgaa gccaaacgcg cggaacttgc cgagcgtgaa
     7201 tgggcgcgaa tgaaagaaaa caactcggca acctgtcgct cctgccataa ctacgatgcg
     7261 atggatcatg ccaggcagca tcctgaagcg gcacgtcaga tgaaggtggc agcgaaagat
     7321 aatcaatcct gcatcgactg tcataaaggt attgcccacc agttaccgga tatgagtagc
     7381 ggcttccgta agcagttcga tgagctgcgc gccagtgcta atgacagtgg tgacacgctg
     7441 tactctattg atattaagcc aatttatgcg gcgaaaggcg ataaagaagc ctctggttct
     7501 ctgctgcctg cttcggaagt gaaagtcctt aaacgtgacg gcgactggct gcaaattgaa
     7561 attaccggct ggacggaaag cgccggacgt cagcgtgtac tcacccaatt cccaggtaaa
     7621 cgcatctttg ttgcctcgat tcgtggtgat gtgcagcagc aggtgaaaac gctggagaaa
     7681 accaccgttg ccgacaccaa taccgagtgg agcaagttgc aggccaccgc gtggatgaag
     7741 aaaggcgaca tggtgaacga tatcaaaccg atctgggcct atgcggattc gttgtacaac
     7801 ggcacctgta accagtgcca cggcgcaccg gaaatcgccc actttgacgc taacggttgg
     7861 atcggcacgc tcaacggcat gattggcttt accagcctcg ataaacgtga agaacgcacc
     7921 ttgttgaaat atctgcaaat gaatgagtct gacaccgcag gtaaggctca cggcgataag
     7981 aaggacgaaa aataatgaac aataacgatc tctttcaggc atcacgtcgg cgttttctgg
     8041 cacaactcgg cggcttaacc gtcgccggga tgctggggcc gtcattgtta acgccgcgcc
     8101 gtgcgactgc ggcgcaagcg gcgactgagg ctgtcatctc gaaagagggc attcttaccg
     8161 ggtcgcactg gggggctatc cgcgcgacgg tgaaggatgg tcgctttgtg gcggcaaaac
     8221 cgttcgaact ggataaatat ccgtcgaaaa tgattgccgg attgccggat cacgtacaca
     8281 acgcggcgcg tattcgttat ccgatggtac gcgtggactg gctgcgtaag cgccatctca
     8341 gcgatacctc ccagcgcggt gataaccgtt ttgtgcgcgt gagctgggat gaagccctcg
     8401 acatgttcta tgaagaactg gaacgcgtac agaaaactca cgggccgagt gccttgctga
     8461 ccgccagtgg ttggcaatcg acggggatgt tccataacgc ttcggggatg ctggcgaaag
     8521 ctattgcctt gcatggtaat agcgttggta cgggcggaga ttactctacc ggtgctgcgc
     8581 aggtgatcct gccgcgcgtg gttggctcaa tggaagtgta tgaacagcaa acctcctggc
     8641 cgctggtatt gcagaacagc aaaaccattg tgctgtgggg ctctgatttg ctgaaaaacc
     8701 agcaagcgaa ctggtggtgc ccggatcacg atgtttatga atattacgcg cagctaaaag
     8761 cgaaagtcgc cgccggtgaa attgaggtca tcagcatcga tccggttgtc acatccaccc
     8821 atgagtatct ggggcgcgag catgtgaagc acattgcggt taacccgcaa actgatgtgc
     8881 cgctgcaact ggcgctggcg tatacgctgt acagtgaaaa cctgtacgac aaaaacttcc
     8941 tcgctaacta ctgtgtgggt tttgagcagt tcctgccgta cctgctgggt gagaaagacg
     9001 gtcagccgaa agatgccgca taggctgaaa aactgaccgg cattgatgcc gaaaccattc
     9061 gtgggctggc gcggcagatg gcggcgaaca gaacgcaaat tattgctggc tggtgcgtgc
     9121 agcgtatgca gcacggtgaa cagtgggcgt ggatgattgt ggttctggcg gcgatgctgg
     9181 ggcaaattgg cctgccaggt ggtggctttg gttttggctg gcactataac ggcgcaggca
     9241 cgccggggcg taaaggcgtt attctgagtg gtttctccgg ctctacgtcg attccgcctg
     9301 ttcacgacaa cagtgattac aaaggctaca gcagcaccat tccgattgcc cgttttatcg
     9361 atgcgatcct cgaaccgggg aaagtgatca actggaacgg taaatcggta aaactgccgc
     9421 cgctgaaaat gtgtattttt gctggaacta acccattcca tcgccatcag cagatcaacc
     9481 gcattattga aggctggcgc aagctggaaa cggttatcgc catagataac cagtggacct
     9541 caacctgccg ctttgccgat atcgtgctgc ctgcgaccac gcagtttgag cgtaacgatc
     9601 tcgaccagta cggcaaccac tccaaccgtg gcattatcgc catgaaacag gtggtgccgc
     9661 cgcagttcga ggcgcgcaac gactttgata ttttccgcga gctgtgccgt cgctttaatc
     9721 gcgaagaagc ctttaccgaa gggctggacg aaatgggctg gctgaaacgc atctggcagg
     9781 aaggtgtaca acaaggcaaa ggacgcggcg ttcatctgcc agcgtttgat gacttctgga
     9841 ataacaaaga gtacgtcgag tttgaccatc cgcagatgtt tgttcgccac caggcattcc
     9901 gcgaagatcc ggatctcgaa ccgctgggca cgccgagtgg cctgattgag atctactcga
     9961 aaaccatcgc cgatatgaac tacgacgatt gtcaggggca tccgatgtgg tttgagaaaa
    10021 tcgaacgctc ccacggtggg cccggctcgc agaagtatcc gttgcatctg caatctgtgc
    10081 atccggattt ccgacttcac tcgcagttat gtgagtcgga aactctgcgt cagcaatata
    10141 cggtagcggg taaagagcca gtattcatta acccgcagga tgccagcgcg cgcggtattc
    10201 gtaacggtga tgtggtacgc gtctttaacg ctcgcggtca ggtgttggca ggggcagtgg
    10261 tttctgaccg ctatgcaccc ggcgtggcac gaattcacga aggggcatgg tacgatccag
    10321 ataaaggcgg cgagcctggt gcgctgtgca aatacggtaa ccccaacgtg ttgaccatcg
    10381 acatcggtac atcgcagctg gcgcagttgt tctcccgtga actggacgat gaacaactga
    10441 cgcaaatctc cagtgcgcag atggctgaat ggttttcgtt gctgaaaagc gaaccgccgc
    10501 tcactgcggc ggtgaatgcg ctggaaaacc gtattgccgc tctgacagta cgtgacgatg
    10561 cccgtctgga actggccgct gacttttgcg gcctgtttct gatgaccgac aaacaagcgg
    10621 cgctgccgta tgcatcggcc tacaaacagg acgagcaaga gattaaacgc ttgttagttg
    10681 aggcagggat ggaaaccagt ggcaatttca acgaatcggc agatcatctg gcgatctatc
    10741 tcgagttgtt gagtcatctg catttttcgc tgggagaggg gaccgttcct gcgcgaagaa
    10801 tcgacagttt gcggcaaaaa acactgacgg cgctgcggca atggttacca gagtttgctg
    10861 cgcgttgtcg acagtatgac agctttggtt tttacgcggc actaagccag ttattgctgg
    10921 tgttagtgga gtgcgaccac caaaacagat aacgtcgttt atgcgcctga aaagacgcgt
    10981 ttagcgtcgc atcaggcatt atggcgcagt tgccggatgc gtcatcacat ccggcaaaca
    11041 cacatcacgg atgagctaca aaccgggaaa gccgctggcg tagcaggcgg ttttcctgct
    11101 tcaggtgcgc aatatcatcc attaacgtca gcgccaccgc gatccccggc cagtccagag
    11161 ccagttcatg acgcaggcgt accgcgcgtt gcaccacaat ggcggcatgg tcgtcaaata
    11221 cccaggttgt ttcctggatc tcacgcggtt caaccacccc caaaccgaca atttcattca
    11281 actcctcttc agagatgccg gtatgcaggc aaaattcggt aatagtaaaa gtcaccgtaa
    11341 cattagccat catgctttcc cccaatcttt acgtggatca aaagacgact gggcgtctgc
    11401 cagttgctgc cacagcgcgg cagtgttttc atccggtttc ggcggcatca cgattttcag
    11461 taccgcatac agatcgccgg tctgtttttt gctcaccaga cctttgcctt taacgcgcaa
    11521 tcgttgcccg gcctggctgc ctggcgggat agtcagcaaa atgctttctt tcagtgttgg
    11581 aacggtgact ttagcaccca gcgccgcttc ccacgggcta accggcacca caatttccag
    11641 atcctggccg acaatatcga acagcggatg tggcgcaata tgaatcacca gccacaaatc
    11701 gccatttgga ccaccgtttt cgcccggcgt cccctggcct ttcagacgga tgcgttgacc
    11761 attgccgacg cccgccggga tcttcacatt cagcgttttc gggatttcct gttcgatcat
    11821 gccaaaggcg ttataaaccg gcaggttata gctgatggta cgcttatgct cagtcagcgt
    11881 ttcttcgagg aataccgcca cttcgatttc aatatcgtgg ccgcgtgcgg cggggcgttg
    11941 acggctctgg cgggcatgct gaccgaaaat tgacgagaag atatcgtcaa aatcttcggc
    12001 attaaaactc tgaccgtcgc catgatggaa ctgatggtta aattgcggat cgttgcgatg
    12061 ttgccacatc tgatcatact cagcgcgacg ttgttcatca cttaacactt cccaggcttc
    12121 agcgacctct ttgaagcggg cttcggcatc cggttctttg ctgacatcag gatggtattt
    12181 acgggccagt cgacgatagg cggtcttgat tgtcttgaga tcgtccgtcg gtttcacgcc
    12241 catgatggcg taataatcct ttaattccat agcgttatct cgcgtaaatc aacacaaatt
    12301 gaaggaaccc ctgtaaggta actcctataa gtgtagggta atcctcaaaa tttcatatgc
    12361 caacacagaa tatgttattg aaatcatcgc ggagaggagg tcgccatcaa gatgggttgc
    12421 tgaacatatt ttaaacaggt gaaaaagggt gagcgatttt tgatagttga accaggcact
    12481 ttaagtttaa ctagggcgtc attatttatt aaattttata gacgctatat atgggtagta
    12541 atatacatgg aattagttgc actgcaaata attatttgaa acaggcctgg aacgatataa
    12601 aaaatgagta cgaaaaaaat caaacatatt caatcacgct ttttgaaaac acactggtgt
    12661 gttttatgcg gttatacaat gaactcagac gtaaagtaaa tgaagaggat actccatgtc
    12721 tggaatgtga atcactagaa aaagaatttg aggaaatgca gaatgataat gatctatcat
    12781 tatttatgag aacattgcgt actaatgata cacaaattta ttcaggggtt tcaggaggta
    12841 ttacatatac tatacaatat gttcaaaatg ttgatattgt tagagtgtcc ttgccgggca
    12901 gaggttcaga gtctatcaca gattttaaag gttattattg gtatggcttt atggaatata
    12961 ttgaaaacat tactgcgtgt gatgatattt tttctgagta ttgtttagat gatgaaaata
    13021 tgagtatcca gccagaacag ataaatacgc cgggaatatc tgatttagat actggtttta
    13081 aaagaacatg agtattcatg ggcgctgggc ggccataaga cgcaaaagca agtatacgaa
    13141 gagtttatta aaattgttga taaacaaacg atcgaggctc ttgttgatac ggcattctat
    13201 aatctcaggg aagatgtaaa gacgttattt ggcgttaatc tacaatctga caacaaaatt
    13261 ca
//
LOCUS       DAZPDU010000121        13260 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZPDU010000121 DAZPDU010000000
VERSION     DAZPDU010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13260
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.122"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..781
                     /gene="umuC"
                     /locus_tag="ACHURS_003090"
     CDS             <1..781
                     /gene="umuC"
                     /locus_tag="ACHURS_003090"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFR1238755.1"
                     /translation="VDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLA
                     DTDIRFIRKHFNVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQ
                     AICSYAARAAEKLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDII
                     NAATRSLDAIWQAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLN
                     AKEGRGTLYFAGQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="ACHURS_003091"
     CDS             complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="ACHURS_003091"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFR1238756.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     MISQPFKAKKRDLFGR"
     gene            complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="ACHURS_003092"
     CDS             complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="ACHURS_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFR1238757.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     IGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            3265..3984
                     /gene="fadR"
                     /locus_tag="ACHURS_003093"
     CDS             3265..3984
                     /gene="fadR"
                     /locus_tag="ACHURS_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFR1238758.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(4036..5568)
                     /locus_tag="ACHURS_003094"
     CDS             complement(4036..5568)
                     /locus_tag="ACHURS_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFR1238759.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREMRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            5898..7196
                     /gene="dadA"
                     /locus_tag="ACHURS_003095"
     CDS             5898..7196
                     /gene="dadA"
                     /locus_tag="ACHURS_003095"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFR1238760.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            7206..8276
                     /gene="dadX"
                     /locus_tag="ACHURS_003096"
     CDS             7206..8276
                     /gene="dadX"
                     /locus_tag="ACHURS_003096"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFR1238761.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALVLRVPVVTV"
     gene            complement(8663..10399)
                     /locus_tag="ACHURS_003097"
     CDS             complement(8663..10399)
                     /locus_tag="ACHURS_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFR1238762.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(10494..11408)
                     /gene="ldcA"
                     /locus_tag="ACHURS_003098"
     CDS             complement(10494..11408)
                     /gene="ldcA"
                     /locus_tag="ACHURS_003098"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFR1238763.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRDERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            11508..12119
                     /gene="emtA"
                     /locus_tag="ACHURS_003099"
     CDS             11508..12119
                     /gene="emtA"
                     /locus_tag="ACHURS_003099"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFR1238764.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLEHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(12121..12855)
                     /locus_tag="ACHURS_003100"
     CDS             complement(12121..12855)
                     /locus_tag="ACHURS_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFR1238765.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            13056..>13260
                     /locus_tag="ACHURS_003101"
     CDS             13056..>13260
                     /locus_tag="ACHURS_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFR1238766.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAV"
BASE COUNT         3367 a         3373 c         3487 g         3033 t
ORIGIN      
        1 ggtggattta tcaaatctgg aacgccagcg taaattaatg tctgctctcc ccgtggatga
       61 cgtctggggg attggacggc ggatcagcaa aaaactggac gcgatgggga tcaaaaccgt
      121 tcttgatttg gcggatacag atatccggtt tatccgtaaa cattttaatg tcgtgctcga
      181 aagaacggtg cgtgaactgc gcggtgaacc ctgtttgcaa ctggaagaat ttgcaccgac
      241 aaagcaggaa attatctgtt cccgctcgtt tggtgaacgt atcacggatt atacgtcgat
      301 gcggcaggcc atttgtagtt acgctgcccg ggcggcggaa aaacttcgca gtgagcatca
      361 atattgtcgg tttatctcca catttattaa gacgtcacca tttgcgctca atgaacctta
      421 ttacggcaat agcgcgtcgg taaaactgct gacgcccact caggacagca gggatatcat
      481 taacgccgct acgcgatctc tggatgccat ctggcaagcg ggccatcgct accaaaaagc
      541 gggcgtgatg ctgggggatt tcttcagtca gggagtcgcg cagctcaatt tattcgatga
      601 caacgcaccg cgccccggga gtgagcaatt gatgacggta atggatacac tgaatgctaa
      661 agagggcaga ggaacactct attttgccgg gcaggggatc caacaacaat ggcagatgaa
      721 gcgagccatg ctttcaccac gttatacaac gcgaagttct gatttactgc gggtcaaata
      781 aatatagcgg caggaaaaaa gcgctcccgc aggagcgccg aatggattag cgaccgaaca
      841 gatcacgttt tttcgcttta aacggctggg aaatcatcac cagcaccgcg acaatcaggt
      901 aagcgataaa aataccgagc agccactgcg gcatttccag acctaaaaaa tcccactgac
      961 gctcggcgca atcgccagag gcgacaaaca cttgcggcac ccatttatcc agcggcagcc
     1021 attccgggaa acgaaccata aaatcacagg tggcaaacgg cgaaggatag agctgaagca
     1081 tggtgtgctc gtaagttaac tgcacaccgc ggaacgcact atacaaccag ataaccatcg
     1141 ctacataacg cagcggagtt ttcggggcga tcgcgccaat cagcgcagca cccagaacgc
     1201 cgaataacgc gcagcgttca taaatacaga gcacgcaagg tttcagtaac atcacatgct
     1261 ggaaccacag cgccgtcagt tccagtgcca gagcagtaaa cgccatcaac agccacgcgc
     1321 cccggccttg tgaacattgg ttcaaaaatc gcaacataat catttccctg caatatgcat
     1381 agagtgcgca gtttaaacca attcattcgc tgcgccacca ggaggcagca aaaataggac
     1441 gcaattggac aattatcggg cgaaaaggca aaccgggcat ttatatgccc ggtaagttgt
     1501 tatcaaagcg ttgctatcca gcccatttgc ataaaccatt cggttacagg ggcaagcgta
     1561 aactcgacgc agagcagtcc gacgagtgtc aggacgaggg tgtaaggcag ggccatccac
     1621 accatgcggc cataagagag gcgaatcaat ggcgcgagtg cagaggtcag caggaatagg
     1681 aacgcagcct gaccgttcgg cgtagcgaca gagggcagat tggtaccggt attaatggcg
     1741 accgccagca gctcgtattg cttcaacgtg atagcgccac tttccattgc cgctttcgct
     1801 tcgttgatat aaatcgtccc cacgaagacg ttatccgaaa tggatgacag cagaccgttg
     1861 aatatataga acagcgacag ctgagcatgt tccgatgcct gcaacacaaa ctgaataatc
     1921 ggcgaaaaca gttgttggtc gataatcacc gcgactaccg agaaaaacac cgtcaacagt
     1981 gcggtgaatg gcagagattc ggtgaaggct ttaccgatag catgctcatc ggtgacaccg
     2041 atcaatgatg tcgccagaat aatgactgac aaaccaatca aaccaacttc cgccagatgc
     2101 aacgccagcg cagtcaccaa ccagacgcca ataatcgcct ggacaatcag acggatttta
     2161 tcctgacggg tacgctggtg gcggctttga tcgtcaaact gctgcagcac ttcgcggact
     2221 ttctccggca gcgtttcacc gtagccaaac caacgcagct tctccaccag caggcaggtt
     2281 aacaggccac aaatcagaac cggaacggtc accggcgaca tgcgcaggaa gaaatcgcca
     2341 aaatgccagc cagccgcttt agcgatgatc aggttctgtg gttcgcccac catggtcatc
     2401 acgccgccta atgcggtgcc gacaccggca tgcatcatca ggctacgcag aaagccacgg
     2461 aactgttcca gaaccacttt gtaatgcttg tcgatatgac tatcgtcttg caggtcggta
     2521 tcttcagtac gggaagaggc tacgcggtga taaataccat aaaaaccgac tgcaacgctg
     2581 atcaccaccg ccacgacggt taaggcatcg aggaacgcgg agaggaacgc cgccgccacg
     2641 caaaaagaga gcgacagcag cattttggag cgaatgctta acagcaaacg ggtaaatatg
     2701 aacagcaata gctgtttcat aaaatagata cccgccacca taaacatcag taacagcaag
     2761 acttcaagat ttgccgccac ctcttcacgg acgtgttccg cgctggtcat gccgatgaat
     2821 accgcttcga tagccaacag accaccgggg agcagcgggt agcatttcag ggccatcgcc
     2881 agagtgaaaa taaattccgc taccagcaac cagcccgcga cgaaagggct gatgaggaaa
     2941 attaacgggt ttacgattaa gaaaatgatg agggcgagtt tgtaccagtc gggggactgg
     3001 cccaaaaaat tgcgccatag cgcgcggccc caggagatct ccatgatggt ttcccttacc
     3061 ttacaaataa tcaatgatgt ttttatgttt aaacgcaaag cttaacggtc aggcaggagt
     3121 gaggcaagtc ttgatagtca aggggaaaga gatgcggaaa atgaagcctt gatccctttt
     3181 tcttcttttt gtctgctatc agcgtagtta gccctctggt atgatgagtc caactttgtt
     3241 ttgctgtgtt atggaaatct cactatggtc attaaggcgc aaagcccggc gggtttcgcg
     3301 gaagagtaca ttattgaaag tatctggaat aaccgcttcc ctcccgggac tattttgccc
     3361 gcagaacgtg aactttcaga attaattggc gtaacgcgta ctacgttacg tgaagtgtta
     3421 cagcgtctgg cacgagatgg ctggttgacc attcaacatg gcaagccgac gaaggtgaat
     3481 aatttctggg aaacttccgg tttaaatatc cttgaaacac tggcgcgact ggatcacgaa
     3541 agtgtgccgc agcttattga taatttgctg tcggtgcgta ccaatatttc cactattttt
     3601 attcgcaccg cgtttcgtca gcatcccgat aaagcgcagg aagtgctggc taccgctaat
     3661 gaagtggccg atcacgccga tgcctttgcc gagctggatt acaacatatt ccgcggcctg
     3721 gcgtttgctt ccggcaaccc gatttacggt ctgatcctta acgggatgaa agggctgtat
     3781 acgcgcattg gtcgtcacta tttcgccaat ccggaagcgc gcagtctggc gctgggcttc
     3841 taccacaaac tgtcggcgtt gtgcagtgaa ggcgcgcacg atcaggtgta cgaaacagtg
     3901 cgtcgctatg ggcatgagag tggcgagatt tggcaccgga tgcagaaaaa tctgccgggt
     3961 gatttagcca ttcaggggcg ataatccctt ccgtttaaag agcaaacccc tcaaacgagg
     4021 ggttttttgt tgtttttaca gatttcccat tcttggcggg caacgttcca gcaactcgat
     4081 gctgccgtct tcgttttgct gttcgagcat cacatcaaat ccccacaggc gatgcacatg
     4141 cttcaggact tctttgcgcc cccgatccag cggtgcgcga ttatgtggaa tataacgcag
     4201 cgtcagcgaa cggtcgccgc gcaaatccac gttccagatc tgaatattcg gctccagatt
     4261 acttaagtta tattgcgacg ataaccggtt acgcatctcc cgataacctt cttcattatg
     4321 aatagcggaa atctccagat aattatgccg atcatcatcc agcacggtga agaagcggaa
     4381 atcacgcatc actttcggtg acagaaactg gctgataaaa ctttcatctt tgaaatcacg
     4441 catcgcgaaa tgcagcgttt ccagccagtc ggaaccggcg atatccggga accagtattt
     4501 gtcttcttcc gttggcgact gacaaatccg tttaatatcc tggaacatgg cgaacccgag
     4561 ggcatacggg ttgatgccgc tgtaccacgg gctgttatag gggggctgga agaccacatt
     4621 ggtgtggctg tgcaaaaact ccagcataaa acgttccgtt actttccctt catcatacag
     4681 atggttaagg atggtgtagt gccagaaggt cgcccagcct tcgttcatca cctgagtctg
     4741 tttttgcgga taaaaatact ggctcacctt acgcacaata cgcaggattt cacgctgcca
     4801 tgattccagc agtggcgcat ttttctccat aaaatagagc aggttttctt gtggttcgga
     4861 cggatagcgg cgcgcttcag caaccgtttt ctcttcctcg cgcttcggca gagtacgcca
     4921 gagcatattg acctgacttt gcagatactc ttcgcgactt ttctgccggg ctttctcttc
     4981 ttgcagcgag attttttgcg ggcgtttgta ccggtccacg ccgtagttca tcagcgcgtg
     5041 gcacgagtcc agaagccgtt ctacttcatc cacgccataa cgttcttcgc actcggtaat
     5101 atatttacgg gcaaaaatca gataatcgac aatcgaactg gcgtcggtcc aactgcggaa
     5161 taagtaattg tttttaaaga aagagttatg cccatagcag gcgtgtgcca tcaccagcgc
     5221 ttgcatggta atggtgttct cttccatcag gtaagcgata cacgggttag agttaatgac
     5281 gatttcatag gccagccctt gctgaccgtg cttatacagc cgttcagtct cgataaattt
     5341 tttaccgaat gaccagtgcg gatagttaat tggcatgccg acgctggagt aggcatccat
     5401 catctgttct gaggttatca cttcaatctg gtgcgggtag gtatccagcc ggtagagttt
     5461 cgccacccgg tctatctctg ccagataaac atccagcagg tcgaacgtcc agtcgggtcc
     5521 atcgctcaaa cgtgtggtgt ccttattcat agaatcgatc gtcgccatac gcgcacctca
     5581 ttgttgtcgg cgctctctgt gtggagcacc tcatttcaag catagaacac ctgttaaaaa
     5641 ccgcgtcgcc ggagaatttt tttctttgcg atttcttatt atcagagtgc cactaatccg
     5701 cttctgaacg gaattttatg ctggataaaa agggcgttca gcaggagata ctaaagacgc
     5761 catattgccg cagagtcagg gagatgtgag ccagctcacc ataaaaaagc cgtatgttga
     5821 ataatgtttt caactgagtt atcaagatgt aatcagatta ttattctttt actgtatcta
     5881 ccgttatcgg agtggctatg cgagttgtca tactgggaag tggtgtggta ggcgttgcca
     5941 gcgcctggta cttaaatcag gcaggacatg aggtcaccgt cattgatcgg gagccggggg
     6001 ccgccctgga aaccagcgcc gcgaatgccg ggcaaatctc ccccggatat gctgcaccgt
     6061 gggcggcacc aggtgtgcct ttaaaagcga tcaaatggat gttccagcgc catgcgccgc
     6121 tggcggttcg tctcgacggt acgcagtttc agttgaaatg gatgtggcaa atgttacgta
     6181 actgcgacac cagccactac atggaaaaca aagggcggat ggtgcgtctg gcggaataca
     6241 gccgtgattg cctgaaagca ttgcgcgccg aaaccaatat tcagtatgaa gggcgtcagg
     6301 gtgggacgct gcaactgttc cgtaccgaac aacagtatga aaatgcgacc cgcgatatcg
     6361 ccgtgctgga agatgccggc gtaccgtatc agctgctgga atccagccgc ctggcggaag
     6421 tggagcccgc gctggcagaa gtagcgcaca aactgacggg tggtctgcaa ctgcccaatg
     6481 atgaaaccgg agactgccag ctgtttaccc agaatctggc gcggatggca gagcaggcgg
     6541 gggttaaatt ccgctttaat acgcccgttg accaactgct ttgcgacggc gagcaaatct
     6601 acggcgtgaa gtgtggcgat gaagtgatta aggccgatgc gtatgtgatg gcgtttggtt
     6661 cttactcgac ggcgatgctc aaaggcattg ttgatattcc ggtgtatccg ctgaaaggct
     6721 actcgctgac tattccaatt gcgcaggaag atggtgcgcc agtatccacc attcttgatg
     6781 aaacctacaa aatcgccatt acccgtttcg ataaccgcat tcgtgttggc ggaatggcgg
     6841 aaattgttgg ttttaacacc gagctgttgc aaccgcgtcg tgaaacgctg gaaatggtgg
     6901 ttcgcgatct ctatccacgc ggcggtcatg tcgagcaggc gactttctgg acggggttgc
     6961 gcccgatgac gccagacggt acgccggttg tcgggcgtac acgctttaaa aatctgtggc
     7021 tgaataccgg tcacggcacg ctcggctgga cgatggcttg cggttccggt cagttgttaa
     7081 gcgatctgct ctctggtcgc acgccagcga tcccatatga ggatctaagc gtagcgcgct
     7141 acagccgtgg atttacgcca tcacgtccgg gccatttaca tggcgcacac agctaaggaa
     7201 acgagatgac ccgtccgata caggccagcc tcgatctgca ggcattaaaa cagaatctgt
     7261 ccattgtccg ccaggccgcg ccgcacgcgc gcgtctggtc ggtggtaaaa gcgaacgctt
     7321 acgggcatgg tattgagcgt atctggagcg cgctcggggc caccgatggc tttgcattac
     7381 ttaacctgga agaggcaata acgttacgtg agcgcggctg gaaggggccg atcctgatgc
     7441 tggaaggatt tttccatgct caggatctgg agatttatga ccagcaccgc ctgaccacct
     7501 gcgtacacag caactggcag ctcaaagcac tgcaaaatgc gcggctaaaa gcaccgttgg
     7561 atatttatct taaagtgaac agtgggatga atcggttggg cttccagccc gatcgcgtgc
     7621 ttaccgtctg gcagcagttg cgggcaatgg cgaatgttgg cgagatgacc ctgatgtcgc
     7681 attttgccga agcggaacat cctgatggaa tttccagcgc gatggcgcgt attgagcagg
     7741 cggcggaagg gctggagtgt cggcgttcgt tgtccaattc ggcggcgact ctgtggcacc
     7801 cggaagcgca ttttgactgg gttcggcctg gcattatttt gtatggcgct tcgccgtccg
     7861 gtcagtggcg tgatatcgcc aataccggat tacgtccggt gatgacgcta agcagtgaga
     7921 ttattggtgt ccagacgcta aaagcgggtg agcgtgtggg ctacggcggt cgctatactg
     7981 cgcgcgatga acagcgaatc ggcattgtcg ccgcagggta cgccgacggt tatccgcgcc
     8041 acgcgcctac cggtacccct gttttagtgg acggcgtgcg caccatgacg gtggggaccg
     8101 tctcgatgga tatgctagcg gtcgatttaa cgccttgccc gcaggcgggt attggtacgc
     8161 cggttgagct gtggggcaag gagatcaaaa ttgatgatgt cgccgccgct gccggaacgg
     8221 tgggctatga gttgatgtgc gcgctggtgc tacgcgtccc ggttgtgacg gtgtaacttg
     8281 ttgtaagccg gatcggaggc aacgtcttct gggtgcaaaa aaaatcatcc atccggctgg
     8341 tcagcaactg tagttgttaa tgtgacagag ccattgccca tgatagtgtc cattaaaagg
     8401 atggacacta tttccccgga acctgaactc accgcacagg cgttctacat aaaacgctta
     8461 cgcttcattg ttgactcgaa ctcgacttca gataaatcac gctttaccct tgatggagcc
     8521 tgtacataga tttgtgtaat tgcctgattt tgatatgttc aattcaacat caaatgaagg
     8581 ttaaattatg gacgacaaac aattgcaggc tcaggctgcg ttcagcaaag catcgcaacc
     8641 ggcgatagat gcttcattaa atttaagatt cagcttcctc ttcagccacc cgtacgccaa
     8701 tcttcaacac ttcattatct tctttctcgg ccaccgtcca gatcatcccg gcaaactcta
     8761 cctggtcacc gacaaccggt gccgcgccta acaactgctg gacaatttca cccagcgttt
     8821 gctgcttatc acgatattct cgcccgtctt ccaggccata tatcagcgca acatcagcat
     8881 atttggcgct ggcttcgaga atgaagtcac caaagaagcg ttgatccagc gcgaccggtg
     8941 gcgactggct gaacagttta ccgagcgccg ggagatcgcg ttcccgacca attacacaca
     9001 acacatcgcc ttcgcgcagt cgggtgctgc cggtaggatg aagcaactgg ttatcacgaa
     9061 atagtgccgc aatacgcgtc tcttttggca tatgcagatc gcgcagtgcc gcacctacac
     9121 accatttatc ggcactcaat tgataaacaa actgctccca cggattttcc ggatgaatat
     9181 ccagaccaac gcgtgacact ggacgtccga cgggcggaac caccactttg gcttttttcg
     9241 ccgcccacga gagtgatgtt ccctgcaaga gcagtgaaac cagaaccaca aagaaggcga
     9301 cattaaagaa cagacgtgca ttctccagcc ccgccatcat cgggaacacg gccaggatga
     9361 ttggcaccgc gccgcgtaat cctacccagc tgataaacac gcgctcacgc agattgaaac
     9421 cgcggaaggg gagcaatcca gcaaataccg aaaggggacg ggcgaagaat atcatccatg
     9481 ccgacaaaat gagcgccgga atggcaatag gcagcagatc gcttgggtta accaatagcc
     9541 ccagcaccag gaacatggcg atttgcgcca gccaggcgag gccgtcgaaa ttttgcagga
     9601 tgccgtagcg gttgcgaatc ggccgattac ccagcagaaa accgcacaga taaacagcca
     9661 gaataccgct gccttccagc gccgtggtta aagcaaaaat taaaataccg ccgcttaatg
     9721 ccagcaatgg atataatccg gcaggcaggg cgatgcgatt aatcatttgc agcagtaaat
     9781 aaccaccgcc aagtccgatg acaatcccga ggccaaattg ctgcagaata tcgaccacga
     9841 acatccaact gacactgctt tcatgttgct ggatcatcgc aatcagggta atcgtcagaa
     9901 agaccgccat tggatcatta ctgccggatt caatttccag cgtcgagcca acacgttcgt
     9961 taagcccctt accacccagc agagaaaaga ccgctgcagc atcggtagag ccgacgatag
    10021 cgccgattaa taagccttca atcaaatcaa gattaaacag ccacgccgcc atcatgccgg
    10081 ttaaaccaga ggtgataagc acgcccagcg tcgccagcga cagtgccggt cctaacgcca
    10141 cacgaaagga actggcctga gtgcgcatcc cgccgtcgag caaaataatc gccagtgcca
    10201 ggttacttac catgtaggcg aaggggtaat tatcaaacgg gataccgccg acgccatcga
    10261 ctcctgccag catgccgatc gccagaaaaa taaccagaat aggaatgcca agacgggaag
    10321 aaaatgaact aagtaatata ctgctggtga caaggatgga tcctaagatg aaaaggctaa
    10381 ttattgttgt ggcatccaac ggtcggttac tcctgattac gctgtctctt atataaaccc
    10441 taccatatta gcggcagaga cagcgtttta cttagccctg aagcgtgatt tttttacatt
    10501 ttaagaacag gatgaccgct aatcgtcaac tgagtgcctt cccgggtgtt attcagaatg
    10561 gcatgtgcgc ccaacggcag cgtaaccgtg cgttgttcat gaccaaaatc gaggccggta
    10621 atgagtggaa tcgacaggcg ggaacgcaaa aacgcgtaca ctgactccag gttgtaacct
    10681 gcgtcataat cattgggcgt gctgccgcta aagctaccga gaatgatcgc cttctgacgt
    10741 ggcaaaattc ccgcatgata gagctgcaac agcatacgtt cgtcccggaa agggtgctcg
    10801 ttaatatctt ccagcaccag aataccgttc tcaatttttg gcatccacgg tgtaccgatc
    10861 agtgaaatca gcatcgcaag attgcctccc cacaacgtgc cttcggcccg acatgtcggg
    10921 ccttcccctt gccattcaat ggtgaaggtt tcattgcgta acgccagcca gaaatggtgc
    10981 tcggtaaagg cgttcagttc atccgcgcca aagtttgcta ccagcatggg gccgctaaag
    11041 gtgatgacat tgccatgcgc cagaagaccg cactgaatgg cggtaaaatc gctatgcccg
    11101 caaatgagca acgggtcatg ttgttggcga gccaccagtg cctgccagtc aatatctgcc
    11161 agtaaacgac tggcaccgta accgccgcgt acagccagca cgatggtgtt gggggtggtc
    11221 agtctggcaa gggaattgag atcttccagg cgctccgttt ccgtacccgc aaaacgctca
    11281 caacgacggg caataacctc gacgttattg acctgatgcc cctcgtcggt aaggcgctgg
    11341 ataccacgca gcgcggcgtg ctgtttaatg cagtaacccg atggggcaat taagtgaaac
    11401 agagacatgg caattccttg ctgacaacag aaacgaaatg tatatcatgc cgcttaggtg
    11461 tgccgttgtc acctcaacgg cgattccagg ctataaggat agaagaagtg aaattgagat
    11521 ggtttgcctt tttgattgtg ttattagcgg gttgttcatc aaagcatgac tatacgaacc
    11581 cgccgtggaa cgcgaaagtt ccggtgcaac gtgcgatgca gtggatgcca ataagccaga
    11641 aagccggtgc agcctggggc gtcgatccac aattgatcac ggcgattatc gctatcgaat
    11701 cgggtggtaa tccgaatgcg gtgagtaaat cgaatgccat tggtttgatg cagttaaaag
    11761 cttcaacctc cggacgtgat gtttatcgcc gtatgggctg gagtggtgag ccgacgacca
    11821 gcgagctgaa aaatccggag cgtaatattt caatgggggc ggcttacctg aatattctgg
    11881 aaaccggccc gctggcaggc attgaagatc cgaaggtact gcaatatgcg ctggtggtgt
    11941 catacgctaa cggggcaggt gcgctgctac ggactttctc gtcagatcgg aaaaaggcga
    12001 tcagcaaaat caacgatttg gatgctgacg agttcctcga acacgtagcg cgaaatcacc
    12061 ctgcgccgca ggctccgcgc tatatctaca aacttgagca ggcactggac gcgatgtaaa
    12121 tcagtcgcgc actttgtccg ctttttcccg ggcttctcgc tcgagagaga aaataatccg
    12181 ctgtaattgc cgctccaccg tcgggctgac gttaagaaaa cggaagctca gacggggagt
    12241 ggtgatggtt tcattcttgc catcaatcac tttgcgctcg ctgatagaga ttaactgggc
    12301 gtcaaagtga aaaacacccc attgccccat gttgacttca atctgagcga agcgcatgcc
    12361 ttcttgtaat tcggcaggct ttgctgtttc cagtaatgcg cccatgccgc ctaacgacaa
    12421 atcatacagg cggaaacgta acgtactgtt atccgccagt ttggtctggc aaaaataagg
    12481 cggatggagt ggggcggaga tgcggaaata tcggcgtcgt tgtacaaacc ataaggtggg
    12541 aggcggtacg gtgataaatg ccggaagctg caagtattca ctctgctgta gttgttcaac
    12601 agtaaactcg actttcgcac cctgagtttc ggcggtaatg gtaatgtgct gtgcctttag
    12661 cacagcgatg ttgtcttcgg cttgactgcc gaaatccagc accagtttat ccggggttat
    12721 tgccagtatt ttgctgatca gctgcccgcc attccaactg agacgcaaag gaattgcggc
    12781 tttgtgcaaa tcgcgtaaca cgcccaggac ggctaacgga ttttgtttca ggaactgctc
    12841 atggtaatga ctcacgcgga aaaactcctg actgacaaac tgtctttggt ttatcggtca
    12901 cagttaacaa aacttaatac aaatgcgtga atatttttta catgttgttc ttaaatcagc
    12961 cgacatacgc ccagcatcga tcccgccctt gcctatactt agagcgttga cgtaagcatt
    13021 tcttgcgtcc gattcatcga aacgagggcc tgaacatggg aattattgct tggattattt
    13081 ttggcctgat agccggcatt atcgccaagc taatcatgcc ggggcgtgat ggtggtggat
    13141 ttttcctgac ctgtattctc gggatagtcg gtgcggtggt cggcggctgg ctggcgacca
    13201 tgtttggcat tggcggctcc atcagcggtt ttaatttgca cagcttcctg gtggcggtgg
//
LOCUS       DAZPDU010000122        13214 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZPDU010000122 DAZPDU010000000
VERSION     DAZPDU010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13214
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.123"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..2064
                     /locus_tag="ACHURS_003102"
     CDS             <1..2064
                     /locus_tag="ACHURS_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFR1238767.1"
                     /translation="GYVPPENWERGINAFYTSYYVSQYYSDYKASGNSKSTYVRFNSG
                     LNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGFAQLLGTLRVGDMYTSSDIFDSVR
                     FRGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVTIEQNGFVVYQKEVPPGPFAITDL
                     QLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQPGVSKYDFAAGRSHIEGASKHSD
                     FVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGWNTRIGAISVDATKSHSKQDNGDV
                     FDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVWANNKDNYRRDENDVYDI
                     ADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGRSGSSKDYQLSYSNNLRR
                     ISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDVSTPRRQIYMSNSTTFDDQGFASN
                     NTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPVATVNGSYSQSSTYRQAG
                     ASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQKYRTTNRNGVVVYDGMTP
                     YRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTDQRKPWFIKALRADGQPL
                     TFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQQGLSCTITFGKEIDESR
                     NYICQ"
     gene            2080..3099
                     /locus_tag="ACHURS_003103"
     CDS             2080..3099
                     /locus_tag="ACHURS_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HFR1238768.1"
                     /translation="MEIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     IATYEHNFNDSSCVSTNTITPMSSSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNANLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGNKATIN
                     SKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLAGSNTILKGAENNGVGFMLESNGS
                     PVTFLNITDNSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALEATALI
                     NVKYD"
     gene            complement(3181..3519)
                     /gene="rcnB"
                     /locus_tag="ACHURS_003104"
     CDS             complement(3181..3519)
                     /gene="rcnB"
                     /locus_tag="ACHURS_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFR1238769.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(3738..4562)
                     /gene="rcnA"
                     /locus_tag="ACHURS_003105"
     CDS             complement(3738..4562)
                     /gene="rcnA"
                     /locus_tag="ACHURS_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFR1238770.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEYDYEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            4683..4955
                     /gene="rcnR"
                     /locus_tag="ACHURS_003106"
     CDS             4683..4955
                     /gene="rcnR"
                     /locus_tag="ACHURS_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFR1238771.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      5047..5143
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5178..5966
                     /gene="thiM"
                     /locus_tag="ACHURS_003107"
     CDS             5178..5966
                     /gene="thiM"
                     /locus_tag="ACHURS_003107"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFR1238772.1"
                     /translation="MQVDLLGSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGLGSFVPHFLDALWQLTQEVQA"
     gene            5963..6763
                     /gene="thiD"
                     /locus_tag="ACHURS_003108"
     CDS             5963..6763
                     /gene="thiD"
                     /locus_tag="ACHURS_003108"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFR1238773.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            6828..7646
                     /locus_tag="ACHURS_003109"
     CDS             6828..7646
                     /locus_tag="ACHURS_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFR1238774.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQVRLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            7698..8444
                     /locus_tag="ACHURS_003110"
     CDS             7698..8444
                     /locus_tag="ACHURS_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR1238775.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(8418..9383)
                     /locus_tag="ACHURS_003111"
     CDS             complement(8418..9383)
                     /locus_tag="ACHURS_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFR1238776.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGEQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(9380..10384)
                     /locus_tag="ACHURS_003112"
     CDS             complement(9380..10384)
                     /locus_tag="ACHURS_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFR1238777.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            complement(10381..11658)
                     /locus_tag="ACHURS_003113"
     CDS             complement(10381..11658)
                     /locus_tag="ACHURS_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFR1238778.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSTDEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            11915..12967
                     /gene="fbaB"
                     /locus_tag="ACHURS_003114"
     CDS             11915..12967
                     /gene="fbaB"
                     /locus_tag="ACHURS_003114"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFR1238779.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGTTIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
BASE COUNT         3470 a         3180 c         3372 g         3192 t
ORIGIN      
        1 ggctatgttc caccggaaaa ctgggagcgg ggtattaatg cgttttatac ctcttattat
       61 gtgagtcagt attacagtga ctataaagcg tcgggtaata gtaagagtac atatgtacgt
      121 tttaacagcg ggttaaattt actggggtgg caactgcatt ctgatgccag tttcagtaaa
      181 acaaataaca atccaggggt gtggaaaagc aataccctgt atctggaacg tggatttgcc
      241 caacttctcg gcacgcttcg cgtgggtgat atgtacacat caagcgatat ttttgattct
      301 gttcgcttca gaggtgtgcg gttgtttcgt gatatgcaga tgttgcctaa ctcgaaacaa
      361 aattttacgc cacgggtgca ggggattgct cagagtaacg cgctggtaac tattgaacag
      421 aatggttttg tggtttatca gaaagaggtt cctcctggcc cgtttgcgat tacagatttg
      481 cagttggccg gtggtggagc agatcttgat gtcagcgtga aagaggcgga cggttcggta
      541 accacctatc tggtgcctta tgcagcggtg ccaaatatgc tgcaacccgg cgtgtcgaaa
      601 tatgattttg cggcgggtcg tagccatatt gaaggggcga gcaaacatag tgattttgtc
      661 caggcgggtt atcagtatgg ttttaataac ttattgacgt tgtatggtgg ctcgatggtt
      721 gcgaataatt attacgcgtt cactttgggg actggctgga atacacgcat tggtgccatt
      781 tccgtcgatg ccacgaagtc gcatagtaaa caagacaacg gcgatgtgtt tgacgggcaa
      841 agttatcaaa ttgcctacaa caaatttgtg agccaaacgt cgacgcgttt tggtctggcg
      901 gcctggcgtt attcgtcgcg tgattaccgg acatttaacg atcacgtttg ggcaaacaat
      961 aaagataatt atcgccgtga tgaaaacgat gtctatgaca ttgccgatta ttaccagaat
     1021 gattttggtc gcaaaaatag cttttccgcc aatatgagcc agtcattgcc agaaggttgg
     1081 gggtctgtgt cattaagtac gttatggcga gattactggg ggcgtagcgg cagtagtaag
     1141 gattatcagt tgagttattc caacaacctg cgacggataa gctataccct cgcggcaagc
     1201 caggcttatg acgagaatca tcatgaagag aaacgtttta atatttttat atcgattccc
     1261 tttgattggg gtgatgacgt ttcgacgcct cgtcggcaaa tatatatgtc taactcaacg
     1321 acgtttgatg atcaggggtt tgcctcaaat aatacgggat tatcaggaac agtagggagt
     1381 cgggatcagt tcaattatgg tgtcaacctg agtcatcaac atcagggaaa tgaaacgaca
     1441 gctggagcga atttaacctg gaacgcgccg gtggcaacag tgaatggcag ttatagtcag
     1501 tcgagtactt atcgacaggc tggagccagt gtttcagggg gcattgtcgc ctggtcgggt
     1561 ggcgttaatc tggcgaaccg tctttccgaa acgtttgctg tgatgaatgc gccaggaatt
     1621 aaagatgctt atgtcaatgg gcaaaaatat cgcacaacaa accgtaatgg agtggtggta
     1681 tacgacggaa tgacacctta tcgggaaaat cacctgatgc tggatgtgtc gcaaagcgat
     1741 agcgaagcag aattacgtgg caaccggaaa attgccgcac cttatcgcgg cgcggttgta
     1801 ctggttaatt ttgataccga tcagcgcaag ccctggttta taaaagcgtt aagagcggat
     1861 gggcaaccat taacgtttgg ttatgaagtc aatgatatcc acggtcataa tattggcgtt
     1921 gtcggccagg gaagccagtt atttattcgc accaatgaaa taccgccatc ggttaatgta
     1981 gcaattgata agcaacaagg actttcatgc acaatcacct tcggtaaaga gattgatgaa
     2041 agtagaaatt atatttgcca gtaatagtca ggtggttcta tggaaattcg cataatgcta
     2101 tttatattaa tgatgatggt tatgcctgtg agctatgcgg catgttatag tgagttatct
     2161 gttcagcaca atttggttgt tcagggggat tttgcactta ctcaaacaca aatagcgaca
     2221 tatgagcata attttaatga ttcgtcatgc gtgagtacaa atactatcac ccctatgagt
     2281 tcgtcggata ttattgttgg gctttataac gataccataa aattaaattt acattttgaa
     2341 tggaccaata aaaacaacat cacgttgtca aataatcaga ccagtttcac cagtggttat
     2401 tcagttacgg tgacacctgc ggccagtaat gcaaaagtga atgtttctgc ggggggcggc
     2461 ggttcagtga tgattaatgg tgttgcgaca ttatccagtg cttcatcatc gacacgcggg
     2521 agtgccgcag tacaatttct actgtgttta ttaggtggca agtcatggga tgcatgtgta
     2581 aatagctaca gaaatgcatt ggcacaaaat gctaatctga cattgtcata caacccgata
     2641 accacaacct gcaaaccgga cgatttatta attactttag acagtattcc cgtttcacaa
     2701 ttaccagcca caggtaacaa agcaacaata aatagtaaac aaggggatat tattctgcgt
     2761 tgtaaaaatt tattaggtca acaaaatcaa acatcacgga aaatgcaggt gtatttatca
     2821 agttctgatt tgttagccgg cagtaacacg atcctgaaag gtgcagaaaa taatggagtc
     2881 ggatttatgc ttgaaagcaa tggttctcca gtcacatttt taaatatcac tgataacagc
     2941 aaaggataca caaatttaaa agaaattgcg gcaaagtcaa aacttacaga tacaacggtt
     3001 tcaattccga taactgccag ttactacgtc tacgatacaa acaaagttaa atctggcgca
     3061 ctggaggcaa ccgcattaat caacgtgaaa tacgactaat aacaagcaag acgagcaagt
     3121 ggctaataat aaaaaataac aaggtgtctg catttccccc tcatgatgag ggggcttttt
     3181 ttagcgatga taaaaaatct caccgtcgta ggctttaatg attttaccgt cggtgtcgct
     3241 gatcaacacg tacgcgccac ccatataggt ccagtgcgtc ccggcatcag gcgcgggcag
     3301 gttacgtaac tgccactgct taatgttgta ctcatcggtg cggtacatct ctgggacggt
     3361 atcacctggt ttgaaatgct taaagtcagc aatgaaacta cttaactcat acttagaaat
     3421 tcccgaggta ttggtcgaac ccgcggtggc tggtgcggcc caggcggcac tggtaaccag
     3481 caaaagcaca cccagcaaca tcttattttt aatggtcata aactctccac tttggggatc
     3541 tgtaatatca gtcgcatatc tttcctgcca tacaatataa ctgacaagcg atattttgtt
     3601 agtgatccca tgatttcgta aaaagtgtaa atgaaaggat gccctaaaag catccttagc
     3661 gagcagagag tcagcggaaa tagctgattt aagaatcatc ctgggctgaa gataacatgc
     3721 aagcctaaag gattgagtta tcgcattatg cccatgaagc catgtacgcc catatacaca
     3781 ccgactaagc caatcaacag actggaaaaa tagggggcgc gtttagcgag agtgttaaat
     3841 ccgctccagc gttttgcgac ctgctgaacg ctgattgctg cgccaacgcc tacggtgaca
     3901 agcgttaacg ccaggccaat gctgaaactg acgaccagtg ttgcgcccag tgtcagggct
     3961 ttcaactgaa tgcatatcaa cagcacggta attgctgccg ggcaggggat aaggccaccg
     4021 gttaagccaa atagcaaaat ttgccagttg gtgacctctc taccatcaaa gcgtcgttta
     4081 atgtcattgg catgggctcg tgcatgggca tcctgatact cgccatgttc gtgatggtga
     4141 tgatgtccat ggtcgtggtg atcttcgtga tcgtgatgat gatgctcata atcatactcg
     4201 tgcatattct ccagccagtt gcgttcgccg cgccaggtac gccagaacat ccagaacgcg
     4261 gtgccaataa tgatcactgc ggaaatcagc tggagccacg gttctgctga ttgagcagta
     4321 aagcgcttgc tgatcaccat cccgccaaag gcaattaacc agaccactgc ggtatgcgaa
     4381 atagttgctg ccagtccgag catcaccgct tgtttaatgg tgcctttgat ggcgatgata
     4441 aacgccgcca tcatcgtttt tgagtgccct ggttccaggc catgaagcgc accaagtaag
     4501 atggcgctgg ggatgaagaa ccaggcgttt ccttgctgaa gaagagttgt aaattcggtc
     4561 atgataataa ttcttagtat taattcggca atctgattct actcccccca gtacctgata
     4621 ctacccccca gtagattaat agtgctatga tattttcata ttcttgttaa ccaggtgttg
     4681 ccatgtctca tacaatccgt gataaacaga aactgaaagc gcgtgccagt aagattcagg
     4741 gccaggtcgt ggcgctcaag aaaatgctcg acgagccgca tgaatgcgct gcagttttac
     4801 aacagattgc tgctatccgt ggcgcggtaa acggtctgat gcgggaagtg attaaaggtc
     4861 atctgacgga acacatcgtt caccaggggg atgagctaaa acgtgaagaa gatctggatg
     4921 tcgttctgaa ggtgctggat tcatatatca aataatttat taacgcgatt gtaaaactgc
     4981 cgtttttcct cgtttacaac gcgtgcgctg gacattacca tcctcctctg cgatttatca
     5041 tcgcaaccaa acgactcggg gtgcccttct gcgtgaaggc tgagaaatac ccgtatcacc
     5101 tgatctggat aatgccagcg tagggaagtc acggaccacc aggtcattgc ttcttcacgt
     5161 tatggcagga gcaaactatg caagtcgacc tgctgggttc agcgcaatct gcgcacgcgt
     5221 tacacctttt tcaccaacat tcccctcttg tgcactgcat gaccaatgat gtggtgcaaa
     5281 cctttaccgc taataccttg ctggcgctcg gtgcatcgcc agcgatggtt atcgaaaccg
     5341 aagaggccag tcagtttgcg gctatcgcca gtgccttgtt gattaacgtt ggcacactga
     5401 cgcagccacg cgctcaggcg atgcgtgctg ccgttgagca agcaaaaagc tctcaaacac
     5461 cctggacgct tgatccagta gcggttggtg cgctcgatta tcgccgccat ttttgtcatg
     5521 aacttttatc ttttaaaccg gcagcgatac gtggtaatgc ttcggaaatc atggcattag
     5581 ctggcattgc taatggtgga cggggagtgg ataccactga cgccgcagct aacgcgatac
     5641 ccgctgcaca aacactggca cgggaaactg gcgcaatcgt cgtggtcact ggcgaggtgg
     5701 attatgttac cgatggccat cgcgccgttg gcattcacgg tggcgatccg ttaatgacca
     5761 aagtggtagg aactggctgt gcattatcgg cggttgtcgc tgcctgctgt gcgttaccag
     5821 gcgatacgct ggaaaatgtc gcatctgcct gtcactggat gaaacaagcc ggagagcgcg
     5881 cagtcgccag aagcgagggg ctaggcagtt ttgttccaca tttccttgat gcgctctggc
     5941 aattgacgca ggaggtgcag gcatgaaacg aattaacgct ctgacgattg ccggtactga
     6001 tccgagtggt ggtgcgggga ttcaggccga tcttaaaacc ttctcggcac ttggcgctta
     6061 tggttgctca gttattactg cactggtggc gcaaaatacc cgtggcgtac agtcggtgta
     6121 tcgcattgag cctgattttg tcgccgccca gctcgattcg gtgttcagcg atgtgcgaat
     6181 cgataccact aaaatcggta tgttggcgga aaccgatatt gttgaagcgg tggcagaacg
     6241 gttgcaacgt tatcagatcc aaaacgtggt actcgacacc gttatgctgg caaaaagcgg
     6301 cgacccactg ctttcacctt cggcagttgc cacgctacgc agtcgattat tgccacaggt
     6361 ttcattaata acgccaaact tgcccgaagc agccgccttg ctcgacgcgc cacacgcgcg
     6421 caccgaacag gaaatgctgg aacaagggcg atcgctgttg gcgatgggct gtggcgcagt
     6481 gctaatgaaa ggtggtcatc tggatgatga gcaaagcccg gactggctgt ttacccgcga
     6541 gggtgaacaa cggtttaccg caccgcgcat tatgaccaaa aacacccacg gtactggttg
     6601 tacactctct gcggcgttgg ctgcactacg cccgcgccat acaaactggg ctgacaccgt
     6661 acaggaggca aaaagctggc tttcatcggc gttagcccag gccgacacgc tggaagttgg
     6721 tcacggtatt ggtccggttc accacttcca cgcctggtgg tgagcaccgt actatagctg
     6781 ctttggttat tgtcagcctt tggcactgac attcagggat gaaaccaatg cagctaagaa
     6841 ttaccagtcg aaaaaaactc accgctctat tatgcgctct tgggctaatt tccatcgttg
     6901 caatttatcc gcgtcagacg gtgaattttt tctattcgac agcagttcag attacagact
     6961 acatccactt ctacggttat cgcccggtta aatctttcgc tattcgtatt cctgccagtt
     7021 acaccattca cggaatagat gtttcgcgct ggcaggagcg gatcgactgg cagcgtgtgg
     7081 caaaaatgcg cgataacggt attcgcttac aatttgcctt tattaaggcg acggaaggcg
     7141 aaaagctggt ggacccctat ttttcgcgta actggcaact aagccgcgaa aatggcttgc
     7201 tgcgcggggc atatcattat ttctccccgt cggtagcagc accggttcag gtgcgattat
     7261 ttctgcaaac ggtagatttc tcacaaggcg atttccctgc ggtactggac gtagaagagc
     7321 gaggaaaatt atcggcaaaa gaattacgca agagggtaag tcagtggcta aaaatggtcg
     7381 aaaaaagtac ggggaaaaag ccgattattt actcaggagc cgttttttat cacacgaatc
     7441 tggcgggcta tttcaatgaa tatccgtggt gggtggctca ctactatcaa cgtcgtccgg
     7501 acaatgacgg catggcctgg cgcttctggc agcattccga ccgtggacag gtagatggca
     7561 tcaatggtcc ggtggatttt aatgtgttta atggcacggt ggaggagttg cagggattcg
     7621 ttgatgggat taaagaaacg ccttaaataa gaagcagtga tatactccca ccagactccc
     7681 tggcaggaaa aagacaaatg gaacaagcgc atacccagct gattgcccaa ctgaacgaac
     7741 gtattttagc ggcggataac acgccgcttt atattaagtt tgccgaaacg gtaaaaaatg
     7801 ccgtgcgcag cggggtgctg gagcatggca atattttgcc cggtgagcgt gatttaagtc
     7861 agttaaccgg cgtttcgcgc attacggtgc gcaaggcgat gcaggcgctg gaagaagagg
     7921 gcgtggtgac gcgttcgcgc ggttacggca cgcagatcaa caacatcttc gaatactcgt
     7981 tgaaagaagc gcgaggtttt tctcagcagg tggtgttgcg cgggaaaaag cccgatacgc
     8041 tatgggttaa caagcgtgtc gtgaaatgtc ccgaagaagt cgcgcagcag ctggcggtcg
     8101 aagcaggaag tgatgtcttt ttgcttaagc gtattcgcta tgtcgatgaa gaagcggtat
     8161 cgattgagga atcgtgggtt ccggcgcatt taattcatga tgttgatgcc atcgggattt
     8221 cactttatga ctatttccgc agccagcaca tttacccaca acgtacgcgt tcccgcgtta
     8281 gcgcccggat gccggatgcc gagtttcagt cacatattca gttagatagc aaaataccgg
     8341 tgctggtgat caagcaagtg gcgcttgacc aacagcaacg gcctattgag tacagcatta
     8401 gccactgtcg cagcgatcta tacgtttttg tgtgcgagga gtagttcctc gcgcgttggc
     8461 gcacaatcac cgccccgatg cccgacaacc cacgacgcca ctgcattgcc cagtaatacg
     8521 gcatccgcca gtggcagacc agaggccagc ccggcaagta cgccacctgc atgactgtcg
     8581 cccgccccaa tggtgtctac aacttgcgtt ggaaatgccg gaatgcagcc agaagcgtcg
     8641 ttgctgaaat accatgcgcc ttctttatcg agacgaacga tcaacggcgc ggcaaatttc
     8701 tcctgccatt gctcgccaag tgttgttatc tctgcggata aagcaaaacg ttcggcggca
     8761 atctcagcct cttgacgatt gagcgacact aaaggtcgac aggccatgat ccgcgccagt
     8821 aatgcatccg ggatatcgcc aatacgtggg ccaaaatcga taaacggcgt cacgtcttgc
     8881 agctcttcca gccattccac taacaattcg ccgcagggcg aggccagttg ataaccggaa
     8941 aaatagagca ggctgccagg cgcaacggtt aatcgcgcca gccactggcg attccactga
     9001 ttttccacac cgctgaatga cataaaagtg cgttcgccat ccggctcaac cagcgccaga
     9061 caccagccgt tatcaccttc ggcgttatcg atcaggctga ttaagccctc ttttgccatc
     9121 cggttgcgaa taatctccgc ccacacgccc tgaccgagcg gcaaggcatt acccgcttcg
     9181 atgccgaggc gctttaacgc cacggcaata ttcagtgcgc agccgccaac gttaacgctc
     9241 tgctgtttca gttcgatatc gcacccacgc caggggaggg cataagcgtc ggcgatcacg
     9301 tcaatgaccg ccgcgccgac gaccatcacc ggctgacgcg tggttaattc aggcagcagc
     9361 gtgtgtaatc gagcgccgct cataccgcct cccgttgttg acgatatttc gccagcgctg
     9421 tggcatagcg gttgaaatca agctgattta ccgcatccag ttccgccttt aacgcaggat
     9481 cgatagcgtt aacgccatgc aacgcgccgc aaattgccgt cgccatagca ccgatggtgt
     9541 ctgtgtcgcc gccaaggtta gcgcacagga cggcgcagcg attcgggtcg gtttgtgcta
     9601 gttcaaccag cgcaatggcg cacggaacgg actcaatagt gctggtacct gcgccaacga
     9661 cctggtaaag ctgttcgctg gcggattcgg tgccgtcggc attgcgcaca attttcagcg
     9721 caatctccag ccgcgccgcc agtgaggcgc tgaacgtagt gatgcgtttt tgctgtgcat
     9781 gtcgcgcaat tgaaggcagg gaatcaacaa tcgctgacca gctttctccg tcaatggcac
     9841 gagaaatcgc ccatgcgatg actaccgcgc ctgcaaccgc cagatcggat ttatgggtcg
     9901 ggctggacgc cagcgctaca tcatcaataa aggaatcaac atcacgcgcc ggaagcaaac
     9961 aacctaatgg cgagacgcgc atcgctgcgc cgttggtcac gccgttgttt tccagttcag
    10021 caacgggttt accgtcgcga atggcgttaa gcgcaatctt cgaggtcgga cctagtacgt
    10081 ttttgttaaa ggcgtcgaaa cgcagcgccc agtcgagaat attacgccca atcagatccg
    10141 gatcgatctt gccttcacgt tccagtaacg catccgccag acacagcgcc atcgaggtat
    10201 cgtcggtgaa ttcggcgcgg ttaaaataac aggccgcgtt attctccttt ggtccaggaa
    10261 gaaaacggtc aatccagcca aagtgtgctt taacgcggct gcgtggccaa agctcggagg
    10321 gcatccccat cgcatcccct aacgcctgcc cataaagagc accgagaata cgttctgttt
    10381 tcatttaact tccccttgtg tcaacgcaat atcgcgatca tcgaccttga tagccgtaat
    10441 ttcgttgtcg gattcgcgga aaaaaatcat gaacagcacg gcgataatgg caatcatcac
    10501 cgcgccgaaa gtccacatcc cggaccagtt gaaagtcagt ccgtttaccg gttcctgata
    10561 agcgaacatc ttttccatca tcacaccgcc aagacgatag ccgagcaaac tgccgaagcc
    10621 ctggcagcag agcgtgatca gcccctgcgc agcggtacgc atatgcacgg gggctttttt
    10681 atcgacatag atgtaagcgg taacgtagta aaaatcgtaa cttacgccgt gaagcaaaat
    10741 accgaggaac agtaacgcgt aggtgaaata ttcatccgta ctaccgtaaa taaagaagcc
    10801 atagcggatc gcagcggtga ccagaccaag caataatacc tttttgatac caaagcgttt
    10861 agtgaaaaac ggcaatgcca gcataaagaa gatttcagag aactggccga gcgtcatcca
    10921 gccggtggcg tttttcatgc caacttcggt cagataaccg ttggcaaaga tgtaatagaa
    10981 cgccagtggc atcgcaaaca ggaatgaaca gaagaaaaag acgaggaagt ttttatcgcg
    11041 cagcaggatc agcgcatcca ggccgagcat gactttaata tccattttgc cggtgctttt
    11101 tggtggcgtg tcgggcagga aaaacgcaaa cacaccgagc agagcagaac ttccggcggt
    11161 aatcagcagc gggatgttag tcggtgagat atcggcatac cccagtattt gcggcaagaa
    11221 accacatgcc agaccggagg caatccagcc gatagtgccc atcacacgaa tgcgcgggaa
    11281 atcacgctcc acatccggca cgttggcaaa agcgatgctg ttagtcagcg caatggtcgg
    11341 catataggtt agagagtaag ccagcagtaa cggaaagaac ccggcaaaag tggtctgttg
    11401 cgcagcgaaa tacatcagca gcgcgcctgc gaacatcaat accgccagca ctttttgcgc
    11461 cgagaaaaag cggtcagtga tggagccaac cagaattggc gacaggatcg ccgcaatggc
    11521 ggtacaggca tacgaccagc caatttctcc ggcactaaaa ccgcttttac ttaaccacaa
    11581 ccacaatggc acaaaccacg cgccccagat aaaccattca acaaacatca tgaacgacag
    11641 ctttgctgtt gttttcatct tttaattcct tcatgacagg taatgtaagg tacgcgatga
    11701 caataccact taacaatacc ttttaaatac ctttgcggtg aaaacttgac cgcgctaaca
    11761 ttttttctga tgaatcgagc caacagaaaa cgctgaaaaa acatccaaaa gatggaaaaa
    11821 ctcgcccgta acgaccagga ttatgattgc ccgcaaaagg cgggccagga caatccatag
    11881 ccgatatcca atcggaattt acgggagcat agtaatgaca gatattgcgc agttgcttgg
    11941 caaagacgcc gacaaccttt tacagcaccg ttgtatgaca attccttctg accagcttta
    12001 tctccccgga catgactacg tagaccgcgt aatgattgac aataatcgcc cgccagcggt
    12061 gttacgtaat atgcagacgt tgtacaacac cgggcgtctg gctggcacag gatatctttc
    12121 tattctgccg gttgaccagg gcgttgagca ctctgccgga gcttcatttg ctgctaaccc
    12181 gctctacttt gacccgaaaa acattgttga actggcgatc gaagcgggct gtaactgtgt
    12241 ggcgtcaact tacggcgtgt tggcgtcggt atcgcggcgc tatgcgcatc gcattccatt
    12301 tctcgtcaaa cttaatcaca acgagacgct aagttacccg aatacctacg atcaaacgct
    12361 gtatgccagc gtggagcagg ccttcaacat gggcgcggtg gcggttggta cgactatcta
    12421 ttttggctcg gaagagtcac gtcgccagat tgaagaaatt tctgcggcct ttgaacgcgc
    12481 gcacgagctg ggcatggtga ccgtgctgtg ggcctatttg cgaaactccg cctttaagaa
    12541 agatggcgtt gattaccatg tttccgccga cctgaccggt caggcaaacc atctggcggc
    12601 aaccatcggt gcagatatcg tcaaacaaaa aatggcggaa aataacggcg gctataaagc
    12661 aattaattac ggttacaccg acgatcgtgt ttatagcaaa ttgaccagcg aaaacccgat
    12721 tgatctggtg cgttatcagt tagctaactg ctatatgggc cgggctgggt tgataaactc
    12781 cggcggtgct gcgggcggtg aaactgacct cagtgatgca gtgcgtactg cggttatcaa
    12841 caaacgcgct ggcggaatgg ggctgattct tggacgtaaa gcgttcaaga aatcgatggc
    12901 tgacggcgtg aaactgatta acgccgtgca ggacgtttat ctcgatagca aaattactat
    12961 tgcctgatac gcctcatacg aacagcacca ccccgtagga cggataaggc gtttacactg
    13021 catccggcaa cctatgcctg atgcgacgcg gaagcgtctt atcatgccta catagcactg
    13081 ccacgtatgt cggaaaaggc gtttacaccg catccggcat aaaaacacgc gcactttgct
    13141 acggcttccc tatcgggagg ccgttttttt gcctttcact cctcgaacag ttttcatatt
    13201 gtcgttttgt gatc
//
LOCUS       DAZPDU010000123        13106 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZPDU010000123 DAZPDU010000000
VERSION     DAZPDU010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13106
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.124"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            134..433
                     /locus_tag="ACHURS_003115"
     CDS             134..433
                     /locus_tag="ACHURS_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1238780.1"
                     /translation="MGNGNFWQLFLGKEPYSKKSDFVKAWLLWWAITILVLFILSLTT
                     TLNLRSIGIGLSFGFCQILGRYTMSLGWSKYWGVLGVFPVTNIVLFLILIFFKKR"
     gene            complement(480..1367)
                     /gene="rarD"
                     /locus_tag="ACHURS_003116"
     CDS             complement(480..1367)
                     /gene="rarD"
                     /locus_tag="ACHURS_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFR1238781.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(1419..1886)
                     /gene="yigI"
                     /locus_tag="ACHURS_003117"
     CDS             complement(1419..1886)
                     /gene="yigI"
                     /locus_tag="ACHURS_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFR1238782.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            2051..2920
                     /gene="pldA"
                     /locus_tag="ACHURS_003118"
     CDS             2051..2920
                     /gene="pldA"
                     /locus_tag="ACHURS_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFR1238783.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYCFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            3053..4882
                     /gene="recQ"
                     /locus_tag="ACHURS_003119"
     CDS             3053..4882
                     /gene="recQ"
                     /locus_tag="ACHURS_003119"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFR1238784.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4946..5563
                     /gene="rhtC"
                     /locus_tag="ACHURS_003120"
     CDS             4946..5563
                     /gene="rhtC"
                     /locus_tag="ACHURS_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFR1238785.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLMVGGGLYLCWMGYQMLRGALKKEAVSAPA
                     PQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVETL
                     AWFIVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5625..6245)
                     /gene="rhtB"
                     /locus_tag="ACHURS_003121"
     CDS             complement(5625..6245)
                     /gene="rhtB"
                     /locus_tag="ACHURS_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFR1238786.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6356..7378
                     /gene="pldB"
                     /locus_tag="ACHURS_003122"
     CDS             6356..7378
                     /gene="pldB"
                     /locus_tag="ACHURS_003122"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFR1238787.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7386..8186
                     /gene="yigL"
                     /locus_tag="ACHURS_003123"
     CDS             7386..8186
                     /gene="yigL"
                     /locus_tag="ACHURS_003123"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFR1238788.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNLDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            8262..9161
                     /gene="bioP"
                     /locus_tag="ACHURS_003124"
     CDS             8262..9161
                     /gene="bioP"
                     /locus_tag="ACHURS_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFR1238789.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(9049..10002)
                     /gene="metR"
                     /locus_tag="ACHURS_003125"
     CDS             complement(9049..10002)
                     /gene="metR"
                     /locus_tag="ACHURS_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFR1238790.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            10238..12499
                     /gene="metE"
                     /locus_tag="ACHURS_003126"
     CDS             10238..12499
                     /gene="metE"
                     /locus_tag="ACHURS_003126"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFR1238791.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12621..12743)
                     /locus_tag="ACHURS_003127"
     CDS             complement(12621..12743)
                     /locus_tag="ACHURS_003127"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1238792.1"
                     /translation="MRKNDSVISVSERAQIAKWLPVMEAIRLLAKCALFMPDAA"
     gene            complement(12784..13029)
                     /locus_tag="ACHURS_003128"
     CDS             complement(12784..13029)
                     /locus_tag="ACHURS_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFR1238793.1"
                     /translation="MITETEINVLKIMAEKDINWNWMNLDRTLSIRQIPGFGNVVNIV
                     NKLANEGLVIIEDSGHKSMPHYHVTEKGYNFVKSENK"
BASE COUNT         3102 a         3256 c         3491 g         3257 t
ORIGIN      
        1 cagtctttgc attgataact atacagtagt aatttagagt gtgtaagttt ttattgatgg
       61 ggtatatttt aattaaatat aattgaaatg tatttttatt ctataaacaa tatcttgggt
      121 gaatggattc tatatgggaa atggtaactt ttggcagttg ttcttgggta aagaacctta
      181 cagcaaaaaa tctgattttg ttaaggcatg gttattgtgg tgggctatta ccatccttgt
      241 tctatttatt ttaagcctga ctacgacatt aaatttaaga agtataggta tagggttaag
      301 ttttgggttt tgtcaaatcc ttggtagata tacgatgtct ctaggatgga gtaagtactg
      361 gggagttttg ggggtatttc ctgtaacaaa tattgttctt tttcttatct taattttttt
      421 caaaaaaaga taaataagtc agtgggtagg ctctttaaac ggcctaccca tgttaattct
      481 taactttttc tacgctgcgt ataaatcgca tccatcacaa aaattgccag cgccacccaa
      541 ataaaggcga aagtcaccat cttatcggca cccggttttt caccataaaa cgtcacagcc
      601 agcaggaaca tcagcgtcgg gccaatgtac tggaaaaagc ctaacgttga gagacgcaag
      661 cgcgtggcag cggcggtaaa acacaacagc ggtacggtag tgacaatacc ggcggcgatc
      721 agcagtaaat tcagcgacat cgggttttgc cccatatggc tggttgagct gtcggcaata
      781 gcaaacaggt aaattgccgc cacgggcagc agccacatgg tttcgattaa catgccggtt
      841 tgcgcttcaa cggcaatctt cttgcgtacc agaccgtaga aggcaaaact aaatgccagt
      901 cccagcgcga taataggtag cgaaccaaaa gtccacagct ggactaacac gccacatatc
      961 gccagaatca ccgccagcca ttgcatccgg cggaatcgct cgccgaggaa aatcatcccc
     1021 agcacaatgt tcaccagcgg gttaataaag taaccaaggc tcgcttccag catatggtga
     1081 ttgttcaccg cccagataaa cagtagccag ttgccaccaa tcagcacggc agagactgcc
     1141 agcataaaaa ttttctgtgg cgtctgaatc agcgttttta aataggacca ctggcggcaa
     1201 atgctcatca gcaccaccat aaagaaaaac gaccagatca cgcgatgcgt caggatttca
     1261 tcggcgggca cgtagtaaat caacttgaag tacgctggcg ctatacccca aataaaataa
     1321 gcggcaagag cgagtaatac gccctgccgc gtttgttttg catccatcgg gaatactcat
     1381 ttttaattgg taacagcagt ttacctgctt ttatgtcttc aacctaccat ataggtggcg
     1441 gtggcactgg caatataaag ctgttcttca ttgtgtaatt caacgcgggc gacggcgact
     1501 ttattgcctg cacgcaacag gctactggta gcagtaaaac gctcgcccct gcctgggcgc
     1561 agataatcaa cgcgaagatc aatggtcccc atccgcgata gccgctggcg tagttcatct
     1621 tcactgatgg tttcgtggcg ggttaaggta cttcccacgc acaccagacc ggcggcgaca
     1681 tccagcgccg acgcaatgac cccgccgtgc aaaatgcttt gcgcccagtt gcccaccatc
     1741 attggctgat ttttaaaggc cagctgtgcg aactcttttt cgtaacgctc cagttccatc
     1801 cccaatgcgc ggttaaatgg catgtgataa acaaacatct cacccactaa tttcagggct
     1861 tgttcagcgg tcagtacggc agacatatca tccttacact tcattggtta atgaaatgtt
     1921 gattttatgc ttctttgttg ttggtttcta ctttaggaag ggataactaa cggctatgga
     1981 gttaagtatg taaaatagcc cgcagaaaaa tattcacctt atcaataatt cgttacggag
     2041 aacacgaccg atgcggactc tgcagggctg gttgttgccg gtgtttatgt tgcctatggc
     2101 agtatatgca caagaggcaa cggtgaaaga ggtgcatgac gcgccagcgg tgcgtggcag
     2161 tattatcgcc aatatgctgc aggagcatga caatccgttc acgctctatc cttatgacac
     2221 caactacctc atttacaccc aaaccagcga tctgaataaa gaagcgattg ccagttacga
     2281 ctgggcggaa aatgcgcgta aggatgaagt aaagtttcag ttgagcctgg catttccgct
     2341 gtggcgtggg attttaggcc cgaactcggt gttgggtgcg tcttatacgc aaaaatcctg
     2401 gtggcaactg tccaatagcg aagagtcttc accgtttcgt gaaaccaact acgaaccgca
     2461 attgttcctc ggttttgcca ccgattactg ttttgcaggt tggacgctgc gcgatgtgga
     2521 gatggggtat aaccacgact ctaacgggcg ttccgacccg acctcccgca gctggaaccg
     2581 cctttatact cgcctgatgg cagaaaacgg taactggctg gtagaagtga agccgtggta
     2641 tgtggtgggt aatactgacg ataacccgga tatcaccaaa tatatgggtt actaccagct
     2701 taaaatcggc tatcacctcg gtgatgcggt gttgagtgcg aaaggacagt acaactggaa
     2761 taccggttac ggcggcgcgg agttaggctt aagttacccg atcaccaaac atgtgcgcct
     2821 ttatactcag gtttacagcg gctatggcga atcgctcatc gactataact tcaaccagac
     2881 ccgtgtcggt gtaggagtta tgctaaacga cttgttttga tgaacggttg agtggttggc
     2941 aaatctggaa cccagcatcc aggattaccc tctcagagac taaaagcatt gcagtttctc
     3001 gcgcaggcgc tgaaaatagc gcctgttttt atttcaggca atcggggtga atgtggcgca
     3061 ggcggaagtg ttgaatctgg agtccggagc taaacaggtt ttacaagaaa cctttggcta
     3121 ccaacagttt cgccccggcc aggaagaaat tatcgacact gtgctttccg gtcgcgattg
     3181 cctggtcgtc atgcccaccg gtggcggaaa atccctttgc tatcaaattc ctgccttatt
     3241 gctaaacggc cttaccgtgg ttgtttcacc gctgatttcg ttgatgaaag atcaggtgga
     3301 tcaactgcaa gccaacggcg tggcggcggc gtgccttaac tcgacgcaaa cccgcgagca
     3361 gcaacttgaa gtgatgacag gctgccgcac cgggcaaatt cgtctgcttt atatcgcccc
     3421 ggaacgcctg atgctggata actttcttga gcatctggcg cactggaatc cggtgttatt
     3481 agccgttgat gaagcgcact gtatctccca atggggccac gatttccgcc cggaatatgc
     3541 cgcgcttggt cagttacgcc agcgtttccc gaccctgccg tttatggcgc tgactgccac
     3601 agccgacgac accacgcgcc aggatatcgt gcgcttgctg gggctgaacg atccgctgat
     3661 tcaaatcagc agttttgacc gtccgaatat tcgctacatg ctgatggaga aattcaaacc
     3721 gctcgatcag ttgatgcgct acgtgcagga acagcgcggt aagtccggca ttatctactg
     3781 caacagccgg gcgaaagtag aagacaccgc tgcgcgcctg caaagcaagg gtattagcgc
     3841 ggcggcctat catgccgggc tggaaaataa tgtccgcgcc gacgtgcagg agaaattcca
     3901 gcgcgatgac ctgcaaattg tggtggcgac ggtggcgttc ggcatgggca tcaataaacc
     3961 aaacgttcgc ttcgtggtcc actttgatat tccgcgcaat atcgaatcct attatcagga
     4021 aaccggtcgc gccgggcgtg atggcctgcc tgcggaagcg atgctgtttt acgatccggc
     4081 tgatatggcg tggctgcgcc gttgtctgga agagaagccg caggggcagt tgcaggatat
     4141 tgagcgccac aaactcaatg cgatgggcgc gtttgccgaa gcgcaaactt gccgtcgtct
     4201 ggtattgctg aactattttg gcgaagggcg tcaggagccg tgcgggaact gcgatatctg
     4261 cctcgatccg ccgaaacagt acgacggttc aaccgatgct cagattgccc tttccaccat
     4321 tggtcgtgtg aatcagcggt ttgggatggg ttatgtggtg gaagtgattc gtggtgctaa
     4381 taaccagcgt atccgcgact atggtcatga caaactgaaa gtctatggca tgggccgtga
     4441 taaaagccat gaacattggg tgagcgtgat ccgccagctg attcacctcg gcctggtgac
     4501 gcaaaatatt gcccagcatt ctgccctaca actgacagag gccgcgcgcc cggtgctgcg
     4561 cggcgaatcc tctttgcaac ttgccgtgcc gcgtatcgtg gcgctcaaac cgaaagcgat
     4621 gcagaaatcg ttcggcggca actatgatcg caaactgttc gccaaattac gcaaactgcg
     4681 taaatcgatt gccgatgaaa gtaatgtccc gccgtacgtg gtgtttaacg acgcaacctt
     4741 gattgagatg gctgaacaga tgccgatcac cgccagcgaa atgctcagcg ttaacggcgt
     4801 tgggatgcgc aagctggaac gctttggtaa accgtttatg gcgctgattc gtgcgcatgt
     4861 cgatggcgac gacgaagagt agtcagcagc ataaaaaagt gccaggatga agactccgta
     4921 aacgtttccc ccgcgagtca aatgtatgtt gatgttattt ctcaccgtcg ccatggtgca
     4981 cattgtggcg cttatgagcc ccggtcccga tttctttttt gtctctcaga ccgctgtcag
     5041 tcgttcccgt aaagaagcga tgatgggcgt gctgggcatt acctgcggtg taatggtttg
     5101 ggctgggatt gcgttgcttg gcctgcattt gattatcgaa aaaatggcct ggctgcatac
     5161 gcttatggtt ggcggtggcc tgtatctctg ctggatgggt taccagatgc tgcgcggcgc
     5221 gctgaaaaaa gaggcggttt ctgcacctgc gccacaggtc gagctggcga aaagtgggcg
     5281 cagtttcctg aaaggtttac tgaccaatct cgctaatccg aaagcgatta tctactttgg
     5341 ctcggtgttc tcattgtttg tcggtgataa cgttggcact accgcgcgct ggggcatttt
     5401 tgcgctgatc attgtcgaaa cgctggcgtg gtttatcgtc gttgccagcc tgtttgccct
     5461 gccgcaaatg cgccgtggtt atcaacgtct ggcgaagtgg attgatggtt ttgccggggc
     5521 gttatttgcc ggatttggca ttcatttgat tatttcgcgg tgatgccaga cgcgtcttca
     5581 gagtaagtcg gataaggcgt ttacgccgca tccgacatta tttttcacgc atgcctcgcc
     5641 gatgctaaca gcgctcccac cagcataaac aacgagccga aaatcttatt cagcgccttc
     5701 atctgctttg gtcctttaat ccatagagca atccgttgag caagggtggc gtaaccgatc
     5761 atcacaataa tatcgaccac aatagtggtg acgccgagca cgatatactg catcagttgc
     5821 ggctgttgcg gcatgatgaa ttgcggaaat agcgccgcca gaaacacaat acttttggga
     5881 ttggtgagat tcacaaaaac tgcgcgctgg aacaaatgtc gacgcgattg agtagaggcc
     5941 agcgatttaa ggtcaattgc accagcggcg cgccactgct ggattcccag ccaaatcaag
     6001 taagccgcgc ctgcccactt caacacttca aacgcaatca ctgagcggga aaatagcgtc
     6061 cccaacccca cgccaaccag cacaatatga atcgccagtc cggtctgaag cccagcaata
     6121 gacgccaccg cgccgcgata accgtggttg agcgaggtgg tcatagtgtt gattgcacca
     6181 gagcctggcg acaggcttaa aatgatcgat gtcagcaggt aggcaaacca ccattctaag
     6241 gtcatgatga actcccggtg tgtctatttt tgtgccacaa tacgctactg tcgcagcgtt
     6301 gtgtcaggca cgctaaaaaa aacgatttta cgtggtttaa gaggcagatt acccgatgtt
     6361 tcagcagcaa aaagactggg aaacaagaga aaacgcgttt gctgctttta ccatgggacc
     6421 gctgactgat ttctggcgtc agcgtgatga agcagagttt actggtgtgg atgacattcc
     6481 ggtgcgcttt gtccgttttc gcgcacagca ccatgaccgg gtggtagtca tctgcccggg
     6541 gcgtattgag agctacgtaa aatatgcgga actggcctat gacctgttcc atttggggtt
     6601 tgatgtctta atcatcgacc atcgcgggca gggacgttcc ggtcgcctgt tagccgatcc
     6661 gcatctcggg catgttaatc gctttaatga ttatgttgat gatctggcgg cattctggca
     6721 gcaggaggtt cagcccggtc cgtggcgtaa acgctatata ctggcacatt cgatgggcgg
     6781 tgcgatctcc acattatttc tgcaacgcca tccaggtgta tgtgacgcca ttgcgctaac
     6841 tgcgccaatg tttgggatcg tgattcgtat gccgtcattt atggcacggc agatcctcaa
     6901 ctgggccgaa gcgcatccac gtttccgtga tggctatgca ataggcaccg ggcgctggcg
     6961 cgcgttgccg tttgctatca acgtactgac ccacagcaga cagcgatatc gacgtaactt
     7021 acgcttctat gctgatgacc caacgattcg cgtcggtggg ccgacctacc attgggtacg
     7081 cgaaagtatt ctggctggcg aacaggtgtt agccggtgcg ggtgatgacg ccacgccaac
     7141 gcttctcttg caggctgaag aggaacgcgt ggtggataac cgcatgcatg accgtttttg
     7201 tgaactccgc accgccgcgg gccatcctgt cgaaggagga cgaccgttgg taattaaagg
     7261 tgcttaccat gagatccttt ttgaaaagga cgcaatgcgc tcagtcgcgc tccacgccat
     7321 tgttgatttt ttcaacaggc ataactcacc cagcggaaac cgctctacag aggtttaaat
     7381 ttcttatgta ccaggttgtt gcgtctgatt tagatggcac gttactttct cccgaccata
     7441 cgttatcccc ttacgccaaa gaaaccctga agctgctcac cgcgcgcggc atcaactttg
     7501 tgtttgcgac cggtcgtcac cacgttgatg tggggcaaat tcgcgataat ctggagatca
     7561 agtcgtacat gatcacctcc aacggcgcgc gcgttcacga tctggatggc aatctgattt
     7621 ttgctcataa cctggatcgc gacattgcca gcgatctgtt tggcgtagtc aacgacaatc
     7681 tggacatcat tactaacgtt tatcgcgacg acgaatggtt tatgaatcgc catcgcccgg
     7741 aagagatgcg cttttttaaa gaagcggtgt tcaaatatgc gctgtatgag cctggattac
     7801 tggagccgga aggcgtcagc aaagtgttct tcacctgcga ttcccatgaa caactgctgc
     7861 cgctggagca ggcgattaac gctcgttggg gcgatcgcgt caacgttagt ttctctacct
     7921 taacctgtct ggaagtgatg gcgggcggcg tttcaaaagg ccatgcgctg gaagcggtgg
     7981 cgaagaaact gggctacagc ctgaaggatt gtattgcgtt tggtgacggg atgaacgatg
     8041 ccgaaatgct gtcgatggcg gggaaaggct gcattatggg cagtgcgcac cagcgtctga
     8101 aagaccttca tcccgagctg gaagtgattg gtactaatgc cgacgacgcg gtgccgcatt
     8161 atctgcgtaa actctattta tcgtaatcgt tctttatttg gtcagttgtc aacctgatac
     8221 ttcgctacaa tggatactcg ttaatcaaag agttttccat tgtggcgcta cttatcatca
     8281 ccacgattct gtgggccttc tcctttagct tttatggcga gtaccttgcg gggcacgtcg
     8341 atagctattt tgcggtgctg gtgcgcgttg gcctggcggc actcgttttt ctgccgtttc
     8401 tgcgtacccg tggcaatagc ctgaaaacgg tcggcctgta tatgctggtg ggcgcgatgc
     8461 agctcggcgt gatgtatatg ctgagtttcc gcgcttatct ctacctgacg gtctccgaat
     8521 tgttgctgtt caccgtactg acgccgctct acatcacgct gatttatgac atcatgagta
     8581 agcgccgtct gcgctggggc tatgctttta gcgccttgct ggcggtgatt ggtgccggga
     8641 ttattcgcta tgatcaggtt accgaccatt tctggactgg cttgttgctg gtgcaactct
     8701 ccaatatcac ttttgccatc ggcatggtgg gttacaaacg cctgatggaa actcgcccga
     8761 tgccgcagca taacgccttt gcgtggttct atcttggcgc gttcctggtg gcggtaattg
     8821 catggttctt gctgggaaat gcgcagaaaa tgccgcaaac cacgttgcaa tggggcattc
     8881 tggtgtttct tggcgtggtg gcttccggga ttggctactt tatgtggaac tacggcgcga
     8941 cgcaggtgga cgccggaacg ctgggcatta tgaataatat gcacgttccg gcagggctgc
     9001 tggtaaacct ggctatctgg caccaacagc cgcactggcc aacgttcatt acaggcgcgc
     9061 tggtgatcct ggcctcactg tgggtgcatc gtaagtgggt cgctccgcgc tcttcacaaa
     9121 cggcagatga tcgcaggcgt gattgcgcgc tgagcgaata aatgcttctg taatcggctg
     9181 acgctgctcg ccatcgcgca cggcggcgta cagtcggctc cacaagcctt cgcccagggt
     9241 tttggtcacc accagaccct ggcgctcaaa actctctact acccaatgcg gtagcgcggc
     9301 aatacccatc cgcgcggcaa ccatctgaat caacaataag gtgttatcga cgcttttcag
     9361 tgacgggctg acgcctgccg gctgaagaaa atgccgccag acatccagtc gactacgctg
     9421 caccggataa atcaacagcg tctcgctggc gagatcttcc ggtgtaattc gcgttttcgc
     9481 cgccagtgga tggtcaggtg ctaacaccag acgcacttca tagtcgaaca tcggcgaata
     9541 atgcaggcca ctgcgcggca ggatatcgga ggtcatcacc agatccagct cgccctgttg
     9601 caacgcaggc tgcgggtcaa acgtcacgcc cgatttaaaa tccatctcta cctgcggcca
     9661 gttcttatgg aaattttcta acgcgggcgt cagccactga atacagctat ggcactcaat
     9721 ggcaatgcgc agacgcgtct gctgcggttc attgcaggct tgcagtgcct ggctgatttg
     9781 cggcagcacc tggtttgcca gttgcaacag gatttctccc tgcggtgtaa agcgtagcgg
     9841 ctggctctta cgcacaaata gccggaagcc aaggcgttgt tccagatcgc taaactggtg
     9901 agacagggcg gattgcgtct gatgcaacgt cgccgcagcg gctgcgagag agccgcagtt
     9961 ccgcaacgct tgtagcgttt tcaggtgttt tacttcgatc atgaaagtcc ttcacttcgg
    10021 catgaataat ttgcgcttga ggaatataca gtaaccgcca attatggatg tgtaaacatc
    10081 tggacggcta aaatccttcg tcttttaaat ttatggtgcg ttggctgcgt ttctccaccc
    10141 cggtcactta cttcagtaag ctcccgggga tgaataaact tgccgccttc cctaaattca
    10201 aaatccatag gatttacata taattagagg aagaaaaatg acaatattga atcacaccct
    10261 cggtttccct cgcgttggcc tgcgtcgcga gctgaaaaaa gcgcaagaaa gttattgggc
    10321 ggggaactcc acgcgtgaag aactgctgac agtagggcgt gaactgcgtg ctcgtcactg
    10381 ggatcaacaa aagcaagcgg gtatcgacct gctgccggtg ggcgattttg cctggtacga
    10441 tcatgtactg accaccagtt tgctgctggg taatgttccg ccacgtcatc agaacaaaga
    10501 cggttcggta gatatcgaca ccctgttccg tattggtcgt ggacgtgcgc cgactggcga
    10561 acctgcggcg gcagcggaaa tgaccaaatg gtttaacacc aactatcact acatggtgcc
    10621 ggagttcgtt aaaggccaac agttcaaact gacctggacg cagctgctgg aggaagtgga
    10681 cgaggcgctg gcgctgggcc acaacgttaa gcctgtgctg ctggggccgg ttacttacct
    10741 gtggctgggg aaagtgaaag gtgaacagtt tgaccgcctt agcctgctga acgacattct
    10801 gccggtttat cagcaagtgc tggcagaact ggcgaaacgc ggcatcgagt gggtacagat
    10861 tgatgaaccc gcgctggtac tggaactacc acaggcgtgg ctggacgcat acaaacccgc
    10921 ttacgacgcg ctccagggac aggtgaaact gctgctgacc acctattttg aaggcgtaac
    10981 gccaaatctc gacacgatta ctgcgctgcc tgttcagggt ctgcatgttg atctcgtaca
    11041 tggtaaagat gacgttgctg aactgcacaa gcgcctgcct tctgactggt tgctgtctgc
    11101 gggtctgatc aatggtcgta acgtctggcg cgccgatctt accgagaaat atgcgcaaat
    11161 taaggacatt gtcggcaaac gtgatttgtg ggtggcatct tcctgctcgt tgctgcacag
    11221 ccccatcgac ctgagcgtgg aaacgcgtct tgatgcagaa gtgaaaagct ggtttgcctt
    11281 cgccctacaa aaatgccatg aactagcact gctgcgcgat gcgctgaaca gtggtgacac
    11341 ggcagctctg gcagagtgga gcgccccaat tcaggcgcgt cgtcactcta ctcgtgtaca
    11401 taatccggcg gtagaaaagc gtctggcggc gatcaccgcc caggacagcc agcgtgcgaa
    11461 tgtctatgaa gtgcgtgctg aagcccagcg tgcacgtttt aaactgccag cgtggccgac
    11521 caccacgatt ggttccttcc cgcaaaccac ggaaattcgt accctgcgtc tggatttcaa
    11581 aaagggcaat ctcgacgcca ataactaccg cacgggcatt gcggaacata tcaggcaggc
    11641 cattgttgaa caggaacgtt tgggactgga tgtgctggta catggcgagg ccgagcgtaa
    11701 tgacatggtg gaatactttg gcgagcacct cgacgggttt gtctttacgc aaaacggttg
    11761 ggtacagagc tacggttccc gctgcgtgaa gccaccgatt gttattggtg acgttagtcg
    11821 cccggcaccg attaccgtgg agtgggcgaa gtatgcgcaa tctctgactg ataaaccggt
    11881 gaaagggatg ctgactggcc cggtgactat tctctgctgg tcgttcccgc gtgaagatgt
    11941 cagccgtgaa accatcgcca aacagattgc gctggcgctg cgtgatgaag tggccgatct
    12001 ggaagccgct ggaattggca tcatccagat tgacgaaccg gctctgcgcg aaggtttacc
    12061 gctgcgtcgt agcgactggg atgcgtatct ccagtggggc gtagaggcct tccgtatcaa
    12121 cgccgccgtg gcgaaagatg acacacaaat ccacactcac atgtgttatt gcgagttcaa
    12181 cgacatcatg gattcgattg cggcactgga cgcagacgtc atcaccatcg aaacctcgcg
    12241 ttctgacatg gagttgctgg agtcgttcga agagtttgat tatccgaatg aaatcggtcc
    12301 aggcgtctat gacattcact cgccaaacgt accgagcgtg gaatggattg aagccttgct
    12361 gaagaaagcg gcaaaacgca ttccggcaga gcgcctgtgg gttaacccgg actgtggcct
    12421 gaaaacgcgc ggctggccag aaacccgcgc ggcactggcg aacatggtgc aggcggcgca
    12481 gaatttgcgt cggggatgat gaatattaac gagtcactgt aaattgcagt acgatgaaag
    12541 aggcaaaact gcctgatact aggcctacat aatttatgca atttattgaa tttttgggtt
    12601 ttgttggccg gacaaggcgt tcacgccgca tccggcatga acaacgcgca ctttgccaat
    12661 aatctgatgg cttccatcac cggaagccat tttgctatct gcgcccgttc agatacagaa
    12721 ataaccgaat catttttacg caaaatataa tcctgagcga tttgttcagt aatcaacgct
    12781 acgttattta ttctcacttt tcacgaaatt gtatcccttt tcagtaacgt ggtagtgagg
    12841 catagactta tgtccgctat cctcaataat aaccaaaccc tcgttagcta atttgttaac
    12901 aatgttaaca acattaccga atcctggaat ttgccttatg gatagtgtgc gatcgagatt
    12961 catccagttc cagtttatat ctttctcagc cataattttc agtacgttaa tttcagtttc
    13021 agttatcata gtcaagtgca tcctggtggt ataagtcatt aagttctccc ttaagtttat
    13081 aatcttctag agtagcagta tgcgta
//
LOCUS       DAZPDU010000124        12998 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZPDU010000124 DAZPDU010000000
VERSION     DAZPDU010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12998
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.125"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..191
                     /locus_tag="ACHURS_003129"
     CDS             <1..191
                     /locus_tag="ACHURS_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1238794.1"
                     /translation="FHQCQNLDGAFVITPPLMPGPALLVDDIVDSAWTLTVLTALLRQ
                     AGCPTVYPLALASTSVKN"
     gene            191..1393
                     /locus_tag="ACHURS_003130"
     CDS             191..1393
                     /locus_tag="ACHURS_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFR1238795.1"
                     /translation="MNLSANAQATLLLTSDFSRAAASEYKPLSNSEWGKFALWLKHQR
                     ISPAELLVPQPQEKLTGWSDPRISQERILGLLARGHSLVLAVDKWQRAGLWILTRGDA
                     DYPVRLKNRLRTDAPPVLFGCGNKALLQAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQ
                     GICVISGGARGIDECAMASALEAGGTAVGVLADSLLKTSTLVKWREGLIAGNLVLISP
                     FYPEVRFTVGNAMARNKYIYCLAESAMVVRAGMTGGTITGAMEALKHQWLPVQVKPNQ
                     DMQSANSRLVENGASWSAEQAENVTIRLPDVPGLMYDRALRNAQPELFSLHEDDANYA
                     VMPAYTPVDFYQLFVAELAILAKESISIERLASCTGLTIEQISVWLNRAEEEGRVIRL
                     GEGHYQFR"
     gene            complement(1439..2032)
                     /gene="yhgN"
                     /locus_tag="ACHURS_003131"
     CDS             complement(1439..2032)
                     /gene="yhgN"
                     /locus_tag="ACHURS_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFR1238796.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            2224..3327
                     /gene="asd"
                     /locus_tag="ACHURS_003132"
     CDS             2224..3327
                     /gene="asd"
                     /locus_tag="ACHURS_003132"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFR1238797.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            3600..5786
                     /gene="glgB"
                     /locus_tag="ACHURS_003133"
     CDS             3600..5786
                     /gene="glgB"
                     /locus_tag="ACHURS_003133"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFR1238798.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            5783..7756
                     /gene="glgX"
                     /locus_tag="ACHURS_003134"
     CDS             5783..7756
                     /gene="glgX"
                     /locus_tag="ACHURS_003134"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFR1238799.1"
                     /translation="MTRLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIEG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALHEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYACACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIALGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            7774..9069
                     /gene="glgC"
                     /locus_tag="ACHURS_003135"
     CDS             7774..9069
                     /gene="glgC"
                     /locus_tag="ACHURS_003135"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFR1238800.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            9069..10502
                     /gene="glgA"
                     /locus_tag="ACHURS_003136"
     CDS             9069..10502
                     /gene="glgA"
                     /locus_tag="ACHURS_003136"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFR1238801.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDNNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            10521..12968
                     /gene="glgP"
                     /locus_tag="ACHURS_003137"
     CDS             10521..12968
                     /gene="glgP"
                     /locus_tag="ACHURS_003137"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFR1238802.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
BASE COUNT         3039 a         3226 c         3576 g         3157 t
ORIGIN      
        1 gtttccacca gtgtcaaaat ctcgacgggg cgtttgttat tacccctcct ttgatgcctg
       61 gtccggcgtt gctggttgac gatatcgtgg attctgcatg gacgctgaca gttctgacag
      121 cactgttacg ccaggcaggt tgcccgacgg tttatcctct tgcccttgct tctacctcgg
      181 taaaaaattg atgaatcttt cagccaatgc acaagcaact ctcctgctaa ccagcgattt
      241 ttctcgcgcg gcggcgagtg agtataaacc tcttagtaat agtgaatggg ggaagtttgc
      301 attatggctg aagcaccaac gtatcagtcc cgccgagctt ctggtgccgc aaccgcaaga
      361 gaaacttaca ggctggagcg atccgcgtat ttctcaggag cgtattcttg gcttgctggc
      421 gcgtggtcat agtctggtgt tggcggtaga taagtggcaa cgcgccggtt tatggatctt
      481 aacccgcgga gatgctgatt atcccgttcg cttgaaaaac cgattgcgaa cggatgcacc
      541 tcccgtttta tttggctgcg ggaataaagc attactgcaa gcggaaggta tggcgattgt
      601 tggctcgcga gatgctccga ctgacgattt gcgctatacc caacaactgg ccgcgaaact
      661 ggcccaacag gggatttgcg ttatctctgg tggtgcgcga ggtattgatg aatgtgcaat
      721 ggcgtcggca ctggaggccg ggggaactgc cgttggcgta ttagctgata gcttgttaaa
      781 aacgagtacg ttagtgaaat ggcgtgaagg gcttatagca ggcaacctgg tgttgatttc
      841 gccgttttac ccagaggtac gtttcaccgt cggcaatgcg atggcgcgaa ataaatatat
      901 ttattgcctt gctgaaagcg caatggttgt acgtgcggga atgaccggtg gaacgataac
      961 cggggcgatg gaggcattaa aacatcagtg gctgcctgtg caggttaaac caaatcagga
     1021 tatgcaatca gccaattcac gattagtaga aaatggggcg tcatggagtg ctgaacaggc
     1081 tgagaatgtg acgatcagac tgccagacgt tcctgggctg atgtatgaca gagcactccg
     1141 taacgctcaa ccagaactgt tttcgctgca tgaagatgac gcaaattacg cagtaatgcc
     1201 cgcgtatacg cctgtcgatt tttatcaact ctttgtggcg gaactggcga tccttgcaaa
     1261 ggaatcgata agtattgaaa ggctggcgtc ttgtactggt ttaaccatcg aacaaattag
     1321 tgtgtggctg aaccgcgcag aagaagaggg aagggttatc cgattgggcg aaggtcatta
     1381 tcagttcagg taataaaaca ggtgccgcgt ctggttgtgt gcagcacctg tttgaacctt
     1441 accccttcat ccacattcga atgccgtcga ggaacatctg ggttgccatc atcaccagaa
     1501 tcaatcccat caggcgttca agtgcgttca cccctttctc gcccagcaga cgtaaaaata
     1561 gcgaagactg tagcaggatg acaaaggtgc cgccccaggc cagcagcaga gcaatcacca
     1621 gatgtcccat ctgattcggg tactgatgag acaacaacat cagcgtggcg agaatagtcg
     1681 gcccggcgac taacggaatt gccaacggca cgataaatgg ctcttcacct gccggaagcc
     1741 cgctgctatt tcctgaagcg ctggggaaaa tcatcttaat ggcgattaga aacagaatga
     1801 tgccgccaga aatggagacg gtttctgctc gcaggctgag aaatgccaga attttctcac
     1861 ccgcaaacag gaacaccagc attaccagga gagcaataag caactctcgc accatgattg
     1921 cccgccgtct tttcggttca gtatgtttca gtacggacat gaaaataggc aggtttccga
     1981 gcggatccat aatcaggatc aataaaactg ctgcagaaat gatttcattc ataactcaaa
     2041 ttccctgata attgccgcgg actttctgcg tgctaacaaa gcaggataag tcgcattact
     2101 gatggcttcg ctatcattga ttaatttcac ttgcgacttt ggctgctttt tgtatggtga
     2161 aagatgtgcc aagaggagac cggcacattt atacagcaca catctttgca ggaaaaaacg
     2221 cttatgaaaa atgttggttt tatcggctgg cgcggtatgg tcggctccgt tctcatgcaa
     2281 cgcatggttg aagagcgcga cttcgacgcc attcgccctg tcttcttttc tacttctcag
     2341 cttggccagg ctgcgccgtc ttttggcgga accactggca cacttcagga tgcctttgat
     2401 ctggaggcgc taaaggccct cgatatcatt gtgacctgtc agggcggcga ttataccaac
     2461 gaaatctatc caaagcttcg tgaaagcgga tggcaaggtt actggattga cgcagcatcg
     2521 tctctgcgca tgaaagatga cgccatcatc attcttgacc ccgtcaatca ggacgtcatt
     2581 accgacggat taaataatgg catcaggact tttgttggcg gtaactgtac cgtaagcctg
     2641 atgttgatgt cgttgggtgg tttattcgcc aatgatcttg ttgattgggt gtccgttgca
     2701 acctaccagg ccgcttccgg cggtggtgcg cgacatatgc gtgagttatt aacccagatg
     2761 ggccatctgt atggccatgt ggcagatgaa ctcgcgaccc cgtcctctgc tattctcgat
     2821 atcgaacgca aagtcacaac cttaacccgt agcggtgagc tgccggtgga taactttggc
     2881 gtgccgctgg cgggtagcct gattccgtgg atcgacaaac agctcgataa cggtcagagc
     2941 cgcgaagagt ggaaagggca ggcggaaacc aacaagatcc tcaacacatc ttccgtaatt
     3001 ccggtagatg gtttatgtgt gcgtgtcggg gcattgcgct gccacagcca ggcattcact
     3061 attaaattga aaaaagatgt gtctattccg accgtcgaag aactgctggc tgcgcacaat
     3121 ccgtgggcga aagtcgttcc gaacgatcgg gaaatcacta tgcgtgagct aaccccagct
     3181 gccgttaccg gcacgctgac cacgccggta ggccgcctgc gtaagctgaa tatgggacca
     3241 gagttcctgt cagcctttac cgtgggcgac cagctgctgt ggggggccgc ggagccgctg
     3301 cgtcggatgc ttcgtcaact ggcgtaatct ttattcatta aatctggggc gcgatgccgc
     3361 ccctgttagt gcgtaataca ggagtaagcg cagatgtttc atgatttacc gggagttaaa
     3421 tagagcattg gctattcttt aagggtggct gaatacatga gtattcacag ccttacctga
     3481 agtgaggacg acgcagagag gatgcacaga gtgctgcgcc gttcaggtca aaaaaatgtc
     3541 acaaccagaa gtcaaaaatc caattggatg gggtgacaca ataaaacagg aagacaagca
     3601 tgtccgatcg tatcgataga gacgtgatta acgcgctaat tgcaggccat tttgcggatc
     3661 ctttttccgt actgggaatg cataaaacca ccgcgggact ggaagtccgt gcccttttac
     3721 ccgacgctac cgatgtgtgg gtgattgaac cgaaaaccgg gcgcaaactc gcaaaactgg
     3781 agtgtctcga ctcacgggga ttctttagcg gcgtcattcc gcgacgtaag aattttttcc
     3841 gctatcagtt ggctgttgtc tggcatggtc agcaaaacct gattgatgat ccttaccgtt
     3901 ttggtccgct aatccaggaa atggatgcct ggctattatc tgaaggtact cacctgcgcc
     3961 cgtatgaaac cttaggcgcg catgcagata ctatggatgg cgtcacaggt acgcgttttt
     4021 ctgtctgggc tccaaacgcc cgtcgggtct cggtggttgg gcaattcaac tactgggacg
     4081 gtcgccgtca cccgatgcgc ctgcgtaaag agagcggcat ttgggaactg tttatccctg
     4141 gggcgcataa cggtcagctc tataaatacg agatgattga tgccaatggc aacttgcgtc
     4201 tgaagtccga cccttatgcc ttcgaagcgc aaatgcgccc ggaaaccgcg tctcttattt
     4261 gcgggctgcc ggaaaaggtt gtacagactg aagagcgcaa aaaagcgaat cagtttgatg
     4321 cgccaatctc tatttatgaa gttcacctgg gctcctggcg tcgccacacc gataacaatt
     4381 tctggttgag ctaccgcgag ctggccgatc aactggtgcc ttatgctaaa tggatgggct
     4441 ttacccacct cgaactactg cccattaacg agcatccctt cgatggcagt tggggttatc
     4501 agccaaccgg cctgtatgca ccaacccgcc gttttggtac tcgcgacgac ttccgttatt
     4561 tcattgatgc cgcacacgca gcgggtctga acgtgattct cgactgggtg ccaggccact
     4621 tcccgaccga tgactttgcg cttgccgaat ttgatggcac gaacttgtat gaacacagcg
     4681 atccgcgcga aggctatcat caggactgga atacgctgat ctacaactat ggtcgccgtg
     4741 aagtcagtaa cttcctcgtc ggtaacgcgc tttactggat tgaacgtttt ggtattgatg
     4801 cgctgcgcgt cgatgcggtg gcgtcaatga tttatcgcga ctacagccgt aaagaggggg
     4861 agtggatccc gaacgaattt ggcgggcgcg agaatcttga agcgattgaa ttcttgcgta
     4921 ataccaaccg tattcttggt gagcaggttt ccggtgcggt gacaatggct gaggagtcta
     4981 ccgatttccc tggcgtttct cgtccgcagg atatgggcgg tctgggcttc tggtacaagt
     5041 ggaacctcgg ctggatgcat gacaccctgg actacatgaa gctcgacccg gtttatcgtc
     5101 agtatcatca cgataaactc accttcggga ttctctacaa ctacactgaa aacttcgtcc
     5161 tgccgttgtc gcatgatgaa gtggtccacg gtaaaaaatc gattctcgac cgcatgccgg
     5221 gcgacgcatg gcagaaattc gctaacctgc gcgcctacta tggctggatg tgggcattcc
     5281 cgggcaagaa actgctgttc atggggaacg aatttgccca aggccgtgag tggaaccatg
     5341 acgccagcct cgactggcat ctgttggaag gcggcgataa ctggcaccac ggtgtccagc
     5401 gtctggtgcg cgatctgaac ctcacctacc gccaccataa agcaatgcat gaactggatt
     5461 ttgacccgta cggctttgaa tggctggtgg tggatgacaa agaacgctcg gtgctgatct
     5521 ttgtgcgtcg cgataaagag ggtaacgaaa ttatcgttgc cagtaacttt acgccggtgc
     5581 cgcgtcatga ttatcgcttc ggcattaatc agccaggcaa atggcgtgaa atcctcaata
     5641 ccgattccat gcactatcac ggcagtaatg caggcaatgg cggcacggta cacagcgatg
     5701 agattgccag ccacggtcgt cagcattcac taagcctgac actaccaccg ctggccacta
     5761 tctggctggt tcgggaggca gaatgacacg actcgccatt ggcaaacccg ctcccctcgg
     5821 cgcgcattac gacggtcagg gcgtcaactt cacacttttc tccgctcatg ccgagcgggt
     5881 agagctgtgt gtctttgacg ccaatggcca ggaacatcgc tatgacttgc cagggcacag
     5941 tggcgacatt tggcacggtt atctgccgga tgcgcgcccg ggtttgcgtt atggttatcg
     6001 cgttcatggc ccctggcaac ccgccgaggg gcatcgcttt aacccggcga agttgttgat
     6061 tgatccttgc gcgcggcaaa ttgaagggga attcaaagat aacccgctgc tgcacgccgg
     6121 tcataatgaa cctgactatc gcgacaacgc cgccattgcg ccgaaatgcg tagtggtggt
     6181 tgatcactat gactgggaag atgatgcccc gccgcgcacg ccgtggggca gcaccatcat
     6241 ttatgaagcc catgttaaag gattaacgta cctgcacccg gagatcccgg tcgagatccg
     6301 tggcacttat aaagccctcg ggcatccggt gatgatcaac tatttgaaac agttgggcat
     6361 taccgcgctg gaactgctgc cggtggcgca gtttgccagt gaaccacgtc tgcaacgcat
     6421 ggggctaagt aactactggg gttacaaccc ggtggcgatg tttgcgctgc atccggcgta
     6481 tgcctgctcg ccagaaacgg cgctgcatga gtttcgcgat gcaatcaaag cactgcataa
     6541 agcgggtatc gaagtcattc ttgatatcgt gctcaaccat agtgcggaac tggacctcga
     6601 cggcccgtta ttctcgctgc gtgggatcga caaccgtagc tattattgga taagagaaga
     6661 cggcgattat cacaactgga ccggttgtgg caacacgctc aatttgagtc atccggcggt
     6721 ggtggattat gcctgcgcct gcctgcgtta ttgggtagaa acctgtcacg tcgatggttt
     6781 ccgctttgat ctggcggcag tcatgggccg tacgccagag ttccgtcagg atgcgccgtt
     6841 gtttaccgct atccagaact gcccggtgct ctcgcaggtg aagttaattg ctgaaccgtg
     6901 ggatatcgcc cttggtggtt atcaggtggg aaatttcccg ccgctgtttg ccgagtggaa
     6961 cgatcatttc cgcgatgctg cccgtcgttt ctggctacat tatgatttgc ctctgggggc
     7021 gtttgccggg cgttttgctg cctccagcga tgtttttaaa cgtaatggtc gtctgccgag
     7081 tgccgcgatt aatctcgtca ctgcgcatga cggttttacg cttcgcgact gcgtttgctt
     7141 caaccataaa cacaatgaag caaacggaga agaaaatcgc gacgggacca acaacaatta
     7201 cagtaacaat catggtaaag aagggttagg cggttctctt gacctggttg aacggcggcg
     7261 cgacagtatt cacgccctgt taacaacgtt gttgctctcc cagggtacgc cgatgttact
     7321 ggccggtgac gaacacggtc acagccagca tggcaataac aatgcctact gtcaggataa
     7381 ccaattaacc tggttggact ggtcgcaggc aagcagtggt ttaaccgcat ttaccgccgc
     7441 gttaatccat ctgcgcaagc gcattcccgc tttggtggag aatcgctggt gggaagaagg
     7501 cgacggcaat gtccgttggc taaatcgata tgctcaacct ttaagcacgg atgagtggca
     7561 aaacgggccg aaacagctgc aaattctgct ctcggatcgc tttttgatcg caattaacgc
     7621 cacgcttgag gtaacagaga ttgttttacc tgctggggag tggcacgcca ttcccccatt
     7681 cgctggagag gataacccag tgattacggc tgtctggcag ggacctgcac acggattgtg
     7741 tgtgttccag agatgataaa aaaggagtta gtcatggtta gtttagagaa gaacgatcac
     7801 ttaatgttgg cgcgccagct gccattgaaa tctgttgccc tgatactggc gggaggacgt
     7861 ggtacccgcc tgaaggattt aaccaataag cgagcaaaac cggccgtaca cttcggcggt
     7921 aagttccgca ttattgactt tgcgctgtct aactgcatca actctgggat ccgtcgtatg
     7981 ggcgtgatca cccagtacca gtcccacact ctggtgcagc acattcagcg cggctggtca
     8041 ttcttcaatg aagaaatgaa cgagtttgtc gatctgctgc cagcacagca gagaatgaaa
     8101 ggggaaaact ggtatcgcgg caccgcagat gcggtcaccc aaaacctcga cattatccgc
     8161 cgttataaag cggaatacgt ggtgatcctg gcgggcgacc atatctacaa gcaagactac
     8221 tcgcgtatgc ttatcgatca cgtcgaaaaa ggcgcacgtt gcaccgttgc ttgtatgcca
     8281 gtaccgattg aagaagcctc cgcatttggc gttatggcgg ttgatgagaa cgataaaatt
     8341 atcgaattcg ttgaaaaacc tgctaaccca ccgtcaatgc cgaacgatcc gagcaaatct
     8401 ctggcgagta tgggtatcta cgtctttgac gccgactatc tgtatgaact gctggaagaa
     8461 gacgatcgtg atgagaactc cagccacgac tttggcaaag atttgattcc caagatcacc
     8521 gaagccggtc tggcctatgc gcacccgttc ccgctctctt gcgtacaatc cgacccggat
     8581 gccgagccgt actggcgcga tgtgggtacg ctggaagctt actggaaagc gaacctcgat
     8641 ctggcctctg tggtgccgga actggatatg tacgatcgca attggccaat tcgcacctac
     8701 aatgaatcat taccgccagc gaaattcgtg caggatcgct ccggtagcca cgggatgacc
     8761 cttaactcac tggtatccgg cggctgtgtg atctccggtt cggtggtggt gcagtccgtt
     8821 ctgttctcgc gcgttcgcgt gaattcattc tgcaacattg attccgccgt attgttaccg
     8881 gaagtatggg taggtcgctc gtgccgtctg cgccgctgcg tcatcgatcg tgcttgtgtt
     8941 attccggaag gcatggtgat tggtgaaaac gcagaggaag atgcacgtcg tttctatcgt
     9001 tcagaagaag gcatcgtgct ggtaacgcgc gaaatgctac ggaagttagg gcataaacag
     9061 gagcgataat gcaggtttta catgtatgtt cagagatgtt cccgctgctt aaaaccggcg
     9121 gtctggctga tgttattggg gcattacccg cagcacaaat cgcagacggc gttgacgctc
     9181 gcgtactgtt gcctgcattt cccgatattc gccgtggcgt gaccgatgcg caggtagtat
     9241 cccgtcgtga taccttcgcc ggacatatca cgctgttgtt cggtcattac aacggggttg
     9301 gcatatacct gattgacgcg ccgcatctct atgatcgtcc gggaagcccg tatcacgata
     9361 ccaacttatt tgcctatacc gacaacgtat tgcgttttgc gcttctgggg tgggttgggg
     9421 cagaaatggc cagcgggctt gacccattct ggcgtcctga tgtggtgcat gcgcacgact
     9481 ggcatgcagg ccttgcgcct gcgtatctgg cggcgcgcgg gcgtcctgcg aagtcggtgt
     9541 ttactgtgca caacctggcc tatcaaggca tgttttatgc acatcacatg aatgacatcc
     9601 aattgccatg gtcattcttt aatattcatg ggctggaatt caacggacaa atctctttcc
     9661 tgaaggccgg tctgtactat gccgatcaca ttacggcggt cagtccaacc tacgctcgcg
     9721 agatcaccga accgcagttt gcctacggta tggaaggtct gttgcaacag cgtcatcgtg
     9781 aagggcgtct ttccggcgta ctgaacggcg tggacgagaa aatctggagt ccagagacgg
     9841 acttactgtt ggcctcgcgc tacacccgcg atacgttgga agataaagcg gaaaataagc
     9901 gccagttaca aatcgcaatg gggcttaagg ttgacgataa agtgccgctt tttgcagtgg
     9961 tgagccgtct gaccagccag aaaggtctcg acctggtgct ggaagcctta ccgggtcttc
    10021 tggagcaggg cgggcagctg gcgctactcg gcgcgggcga tccggtgctg caggaaggtt
    10081 tccttgcggc ggcagcggaa taccccggtc aggtgggcgt tcagattggc tatcacgaag
    10141 cattttcgca tcgcattatg ggcggcgcgg acgtcattct ggtgcccagc cgttttgaac
    10201 cgtgcggctt aacgcaactt tatggattga agtacggtac gctgccgtta gtgcggcgca
    10261 ccggtgggct tgctgatacg gtttctgact gttctcttga gaaccttgca gatggcgtcg
    10321 ccagtgggtt tgtctttgaa gataataatg cctggtcgct gttacgggct attcggcgtg
    10381 cttttgtact gtggtcccgt ccttcactgt ggcggtttgt gcaacgtcag gctatggcaa
    10441 tggattttag ctggcaggtc gcggcgaagt cgtaccgtga gctttactat cgcttgaaat
    10501 agttttcagg aaacgcctat atgaatgctc cgtttacata ttcatcgccc acgcttagcg
    10561 tagaagctct taagcactct atcgcttaca agctgatgtt tacgattgga aaggacccgg
    10621 tcgtcgccaa taaacatgaa tggctgaacg caacgttatt tgctgtgcgc gatcgtctcg
    10681 tggagcgctg gttacgttca aaccgtgccc agttgtcgca agaaacacgt caggtttact
    10741 acctgtcgat ggagtttttg attggccgta cgctctccaa cgccatgttg tcgctaggaa
    10801 tttacgaaga tgtacagggc gcactggaag cgatggggtt aaatctcgaa gagctgattg
    10861 atgaagaaaa tgacccaggc ctcggtaacg gtggcctggg acgtctggcg gcttgcttcc
    10921 ttgattctct ggcgacgtta gggttgccgg ggcgcggtta cggcatccgc tatgactacg
    10981 gtatgttcaa gcagaacatc gttaacggta gccagaaaga gtcgccagac tactggctgg
    11041 aatacggtaa cccgtgggaa ttcaaacgcc acaacacgcg ctataaagtc cgttttggcg
    11101 gtcgcattca gcaggaaggt aaaaaaacgc gctggattga aaccgaagag attctgggag
    11161 tcgcttacga tcagataatc cctggttacg acaccgacgc gaccaacacg ctgcgtttgt
    11221 ggagtgcgca agccagtagc gaaattaacc tcggtaaatt caaccagggt gactacttcg
    11281 cggcagtgga agataaaaac cactccgaga acgtatctcg cgtactgtat ccggatgact
    11341 ccacctactc cgggcgtgag ctgcgcctgc gtcaggaata cttcctggtt tcctcgacca
    11401 ttcaggacat tttaagccgc cattatcagt tgcataaaac ctacgataac ctggcggata
    11461 aaatcgcgat tcatctcaat gatacccatc cggtactgtc gattcctgag atgatgcgtc
    11521 tgctgatcga tgagcaccaa tttagctggg acgacgcgtt tgaggtgtgt tgtcaggtct
    11581 tctcctacac taaccacacg ctgatgagcg aggcgctgga aacctggccg gttgatatgc
    11641 tgggtaaaat tctgccgcgt cacctgcaga tcatctttga aatcaacgac tatttcctga
    11701 aaaccttgca ggaacagtat ccgaacgata ccgatctgct gggacgggcg tcgatcattg
    11761 atgaatccaa cggtcgtcgt gtgcgtatgg cctggctggc ggttgttgtg agccacaaag
    11821 ttaacggtgt atcggaactg cactctaatc tgatggtgca atcgttgttt gccgactttg
    11881 cgaaaatctt cccgggtcgt ttcaccaacg tcaccaacgg tgtgacgccg cgtcgctggc
    11941 tggcggtagc gaacccatcg ctttcagccg tgctggacga acacctgggc cgtaactggc
    12001 gcaccgacct tagcctgctt aatgagctgc aacaacactg tgatttccca atggttaatc
    12061 acgctgtgca tcaggcgaag ctggagaaca aaaagcgtct ggcagagtat atcgcccagc
    12121 agctgaatgt ggtggtgaat ccaaaggcgt tgttcgatgt acaaatcaaa cgtattcacg
    12181 aatacaaacg tcaattgatg aatgtgttgc atgtgattac ccgctataac cgcatcaagg
    12241 ccgacccgga tgcgaagtgg gtaccgcgcg tgaatatttt tggcggtaag gcggcttcgg
    12301 cctattacat ggcgaagcac attattcatt tgatcaatga cgtagcgaaa gtgatcaaca
    12361 acgatccgca gattggcgat aagctgaaag tcgtgttcat cccgaactac agcgttagcc
    12421 tggcgcagtt gatcattccg gcggcagatc tgtctgaaca gatttcgctg gcagggacgg
    12481 aagcttccgg caccagtaac atgaagtttg cgcttaacgg tgcgctgact atcggtacgt
    12541 tggacggtgc gaatgtcgag atgctggatc atgtcggtgc tgacaatatc tttatttttg
    12601 gtaacacagc ggaagaagtg gaagaactgc gtcgtcaggg ctacaaaccg cgtgaatact
    12661 acgagaaaga tgaggagctg catcaggtgc tgacgcaaat cggcagcggt gtattcagtc
    12721 cggaagatcc gggtcgctat cgcgatctgg ttgattcgct gatcaacttc ggcgatcact
    12781 atcaggttct ggcggattat cgcagctatg tcgattgtca ggataaagtc gacgaactct
    12841 acgagcttca ggaagagtgg accgcaaaag cgatgctgaa cattgccaat atgggctact
    12901 tctcttctga ccgtactatc aaagagtacg ccgatcatat ctggcatatc gatccggtga
    12961 gattgtaagt tcaccaataa atagaacggg gccaaagg
//
LOCUS       DAZPDU010000125        12892 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZPDU010000125 DAZPDU010000000
VERSION     DAZPDU010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12892
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.126"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..330)
                     /locus_tag="ACHURS_003138"
     CDS             complement(<1..330)
                     /locus_tag="ACHURS_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HFR1238803.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVCV
                     IWFGGVSLARRLSGMLSAGLHFDHSIYMNASRLQGIHCFDMTGRLQLYIRLYCSRNAA
                     SPYVIRRL"
     gene            complement(532..1176)
                     /gene="cheZ"
                     /locus_tag="ACHURS_003139"
     CDS             complement(532..1176)
                     /gene="cheZ"
                     /locus_tag="ACHURS_003139"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFR1238804.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGLVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKVGVVASQDQVDDLLDSLGF"
     gene            complement(1187..1576)
                     /gene="cheY"
                     /locus_tag="ACHURS_003140"
     CDS             complement(1187..1576)
                     /gene="cheY"
                     /locus_tag="ACHURS_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFR1238805.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1591..2640)
                     /gene="cheB"
                     /locus_tag="ACHURS_003141"
     CDS             complement(1591..2640)
                     /gene="cheB"
                     /locus_tag="ACHURS_003141"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFR1238806.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(2643..3503)
                     /gene="cheR"
                     /locus_tag="ACHURS_003142"
     CDS             complement(2643..3503)
                     /gene="cheR"
                     /locus_tag="ACHURS_003142"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFR1238807.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3522..5123)
                     /gene="tap"
                     /locus_tag="ACHURS_003143"
     CDS             complement(3522..5123)
                     /gene="tap"
                     /locus_tag="ACHURS_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFR1238808.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISTLVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5169..6830)
                     /gene="tar"
                     /locus_tag="ACHURS_003144"
     CDS             complement(5169..6830)
                     /gene="tar"
                     /locus_tag="ACHURS_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFR1238809.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVARVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(6975..7478)
                     /gene="cheW"
                     /locus_tag="ACHURS_003145"
     CDS             complement(6975..7478)
                     /gene="cheW"
                     /locus_tag="ACHURS_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFR1238810.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7499..9463)
                     /gene="cheA"
                     /locus_tag="ACHURS_003146"
     CDS             complement(7499..9463)
                     /gene="cheA"
                     /locus_tag="ACHURS_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFR1238811.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSMAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVGDEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9468..10394)
                     /gene="motB"
                     /locus_tag="ACHURS_003147"
     CDS             complement(9468..10394)
                     /gene="motB"
                     /locus_tag="ACHURS_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFR1238812.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEDLKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10391..11278)
                     /gene="motA"
                     /locus_tag="ACHURS_003148"
     CDS             complement(10391..11278)
                     /gene="motA"
                     /locus_tag="ACHURS_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFR1238813.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11404..11982)
                     /gene="flhC"
                     /locus_tag="ACHURS_003149"
     CDS             complement(11404..11982)
                     /gene="flhC"
                     /locus_tag="ACHURS_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFR1238814.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11985..12335)
                     /gene="flhD"
                     /locus_tag="ACHURS_003150"
     CDS             complement(11985..12335)
                     /gene="flhD"
                     /locus_tag="ACHURS_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFR1238815.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         3020 a         3554 c         3112 g         3206 t
ORIGIN      
        1 gagtcggcga attacatagg ggctcgcagc gttacggctg caataaagcc ggatataaag
       61 ctgcaaccta cccgtcatgt caaaacaatg gatgccttgc aaacgggatg cgttcatata
      121 aatactgtga tcaaaatgca gcccagcgga gagcatgccc gacaatcgac gggccagcga
      181 cacaccgcca aaccagataa cacacacgcc cactaacaaa atcagcaacg aggtcagttc
      241 acgggaacgc gggatttgcc cctcttcccg cgctttttct agtcggtgag gtgtgggggc
      301 ttctgttttg tcgtcgctct cgtcagacac gtcgccaatc ctggaaagag tattaagctt
      361 aaatgatgcc agagcgcaaa gcgttcaatg gtttgagtaa ggggcaaaac aggcgggatt
      421 tagggctttt gctgccacac atcaagcata gtgtgcgttt gtcggatgcg gcgtcatcgc
      481 cttatcagac cgcctgatat gacgtggtca cgccacatca ggcaatacaa atcaaaatcc
      541 aagactatcc aacaaatcgt ccacctgatc ctgactggct accacgccga ctttgctggt
      601 atcgacctga ggtccattaa gcaaactctg gttttcacgt tttggacgcg actcctgttc
      661 cgggatgttt tccaacagca ccatcagcaa ctggcgttcg atctcctgaa tgacatccat
      721 catccgctta atgaccagcc cggtgagatc ctgaaaatcc tgcgccatca tgatttccag
      781 cagttgcgcg ttagtaaagc tggtatgcgc gggtacatct gccagaaatt gtcgtgtatc
      841 tgttaccagt tcacgggcat cggcaaggtc aatcggatcg gcaaaccagt catcccaacg
      901 ttgggttaac gcttttgctg atttctccat ttgatcctga tgcggttgtg acgcctcaac
      961 actgttcagc gcccgctccg cagcctgggc ggtcatctgc acaacatagt acaaacgatc
     1021 gcgcgcatcg gggatggctt ccgccgcttc ggcaatggcc tgatccagcc ccagttcccg
     1081 caaactgtcg cgcagcatac gcgtcaggct gccgatgcgc gcaatgatat cgccagctga
     1141 atgctcgtca gcaggtttga ttgatggttg catcatagtc gcatcctcac atgcccagtt
     1201 tctcaaagat tttgttgagt ttttcctcca gcgtcgcggc ggtaaatggc ttcaccacat
     1261 agccactggc ccccgcttgc gccgcagcaa tgatgttctc tttcttcgct tctgcagtca
     1321 ccattaacac tggcaatgcc gacatcgcgc catccgcacg aattgttttc agcaattcca
     1381 ggccatccat attgggcatg ttccagtcgg agataacaaa tccataaccg cctgcctgca
     1441 acttattgag agcgtcgacg ccatcttccg cttcctcaac attattgaat cccagctctt
     1501 tcagcaggtt acgcactatg cgtcgcatgg tggaaaagtc atccacaacc aaaaatttaa
     1561 gttctttatc cgccatttca cactcctgat ttaaatacgt atcgcctgtc cggcactaat
     1621 ttttgccaac atttgctggc ttacctggct aagatcgacc acttcgcaga caccacccat
     1681 attgatggcc tcgcgcggca tgccgaacac cacgcaactt gcttcgtttt gcgcaagggt
     1741 ccatgccccc gcctgacgca tcgccaacat tcccgccgcg ccgtcgttgc ccataccggt
     1801 caggatcacc ccaaccgcat tacgccccgc ctgtttggcg acagaatgga acaacacatc
     1861 taccgaaggc cgatgacggt taaccgccgg gccgtcgtga attttgattt gataatttgc
     1921 gccactacgc gccagttcca tatgccgatc gcccggcgca atataggcat gccccggcaa
     1981 gacacgttct ccgtcttcgg cttctttaac cccgatctgg caaagcttat taagtctgtc
     2041 ggcaaaagag cgggtgaaac cgggcggcat atgttgggta attaacaatg ccgggctgga
     2101 aagcggcaac ggttgcagta cgtgacgaat tgcctcagtt ccacccgttg aagcaccaat
     2161 cgcaatcagt ttttcagaac tcaacaacgg acccgccttc agcgttgtcg gtgctgacaa
     2221 tggcttatgt gctgcaaggc tcgcctttgc tgccgtacgc accttttcag caatcatttc
     2281 gctatacgcc agcattcctt cgcgaatacc cagttgcggt ttggtgacaa aatctattgc
     2341 ccccagctcc agcgcgcgca gcgtgacttc tgatcctttg ccggtcaagg aagaaaccat
     2401 cacaacgggc attggacgca aacgcattaa tttttcgagg aaatccagtc cgtccatccg
     2461 cggcatttca acatccagcg tcagcacatc gggattgaat ttcttaatca agtcacgcgc
     2521 gaccagcgga tcaggcgcgg tcgccaccat ttccatgtcg ctatggctgt tgatgatttc
     2581 tgtcatgatc tggcgcatca gtgccgaatc atcgacagat aacaccctga ttttgctcat
     2641 cgttaatcct tacttagcgc atacaccgtc tgaccacgca gcgtgaagcg gcgctcaagg
     2701 tggctaaagt tttcagagtg acccgcaaac agcaatccgt cgggtttaag gagcggaaca
     2761 aagcggcgca aaatctcctg ctgggtattt tgatcgaagt agatcatgac gttacgacag
     2821 aagatcgcat caaacggccc cggcacggtg tactgtttcg ccagtagatt cagcggggca
     2881 aaatcaacat agttcgccag ctcctgacgc acgcgtacca gcccttcatg cggtcccgtc
     2941 cctcgcatga aataccgttg cagttgctgc ggcgtcaggt ttttcaactc ttcatggcga
     3001 taaataccgc ttctggcttt ttccagcact tcggtgtcga tatcactggc aaacactttc
     3061 cagcgtccgg gcgcggtgcc caatgtgtca gccagcgtca tcgcaatgct gtacggctct
     3121 tcgccggtcg aagccgccgc gctccatacg cgatactcgc cagaacggcg acgtgcgtga
     3181 tccgcgagca gagggaaatg gtgtgcctca cggaaaaatg ccgtcagatt cgtggtcagc
     3241 gaattgataa acgcctgcca ctcaccgctg tgctgattag attccagcaa gttcagataa
     3301 tgaccgaaat ccgtcagtcc cagcgaacgc aaacgacgaa ccagtcggtt gtaaaccatg
     3361 tcgcgtttat ggtcagccag aacgatcccg gctcgttgat agatcaattg acttatccgc
     3421 cgaaaatgcg cgtcggaaag cgccaggcgc tcggtcatct gtaacaataa agacgtttgc
     3481 ccacagggca gagatgaagt catagcgcct tctcaatcac ttcaggatac cactggcgca
     3541 atttgtaact gcgccgactc atgtcgtgct acttcatgtt cttcaagggt aaataccgcc
     3601 acacgcgacg aaagatggtc ggcctgattc gccagttgtt ccgttgccac cgccgcttct
     3661 tctaccagcg aggcgttctg ctgagtcacc tgatccatct ggctgacagc ctgtgcaacc
     3721 tgctcaatcc cccgctgttg ttcttccgac gcagaggcaa tttctcccat aatgtcgttc
     3781 acgcgggtca ccgaactgac aatatcgatc atggtcgcgg cggcgttatt caccagtttc
     3841 gaaccctgct ggacacgatt gactgactct tcgatcagcc ctttgatctc ttttgctgcc
     3901 tgcgcgctac ggctggcaag attgcgcact tcacctgcca ctaccgcaaa accacgcccc
     3961 tgctctccgg cgcgagccgc ttccactgcc gcattcaggg ccagaatatt ggtctggaaa
     4021 gcaattccgt cgataacgct gataatgtcg ccaatttttt gcgagctggt ggcgatctcc
     4081 tgcatggtgt gagtcatggt actgacctga acaccgcccg cctgcgccgt tgtcgcggca
     4141 ttttttgcca gttccgacgc ctgtcgtgcg ttatcggcgt tttgccctac cgtggcggtt
     4201 aattgctcca tactggcggc cgtttgtgcc agcgatgccg cctgctgttc ggtacgactt
     4261 gagagatcgt tattgcctgc gacaatctcc gcgataccaa tgtgcatctc ctggcttccc
     4321 ttacgcacat cacttaccgt cccacgcaaa gcctgctgca tggtcttcag actggcaaaa
     4381 atggcggtga tctcattacg accatatacc gcaatcggac gcgccagatt acccgcagca
     4441 atgctgtcaa aatggctacc gataatggcc agtggttgaa caatcatctt gcgcgtccac
     4501 cacagcgcac tgctgatgta gatcgccgca acaataatca tgctgataaa caccagtgtc
     4561 gaaatctgat agttacgctg gctttgcgca ctggcggctt ccagcacatg gttgatctcc
     4621 agctgccagg cctcaaagtt aacgtcaaac gcattctgtg atccctgcac cggcgcagtg
     4681 aggaaatccg aaagttgatt actttcaagc caggtcgcct gatgttcgag atcgttgtgc
     4741 cagcgggcaa aacttttctc cgtctctttt tgcaatgccc tgacgtgctc gttgcccgca
     4801 gtcatcgcca taaaactttt aaacagcgtg gtggattgcg tcagactggc gcgcgccgtc
     4861 gtcatcaacg ttttaatgtc atccgccgga tagctaagtg ccgtcagagt gcccgctttg
     4921 ttcagcgcgg tactggcctg taacattacc gcccgagttt gcgctaatgc cgcacgttgc
     4981 tgattgctct gctcgacctg attcagtcgt tgcaaatcgt cgcgaaatgc ccaaaaagac
     5041 atgccgttac tgccaatctg caagatcccg cagagaatca aaattaaaaa cagcgtggtc
     5101 gaaattcgaa tacgattaaa catcaacgct ccccatcagg cggcaatgac cgcgttagta
     5161 aatactcgtc aaaatgtttc ccagtttgga tcttgttcag caattcgcag tcgtggctgt
     5221 gccggtggtt gctcactggc aggacgggat ggtgtttgcg gtttattggt gagtgggctg
     5281 gctgccagac ggaacgcgga aaccgcttgc gttaaacgac tcgcctgttc ttccagcgca
     5341 gcggcggcgg cagctgattc ctgcaccagc gatgcgttct gttgcgtgac gcgatccatt
     5401 tccgaaaccg ccaatgcgac ttgatcgatg ccacggctct gttcatccga tgccgatgca
     5461 atctcgccca taatgtcagt cacgcgagtg acagcattga cgatattgtt cattgtttcc
     5521 ccggcgcttt cgaccagcac cgaaccggta tcaacgcgtg cgacggagtc ttcaatgagg
     5581 gctttgatct cttttgccgc ctgggcgctg cgactggcaa gattacgcac ttcacccgcc
     5641 accacggcaa aaccacggcc ctgttcaccc gcacgcgcgg cttcaaccgc ggcattcagc
     5701 gcgaggatat tagtctggaa ggcaataccg tcgataacgc tgataatgtc ggcaattttc
     5761 ttcgaactat cggcgatctc atgcatcgtt ttcactacgc catccaccac tttgccgccg
     5821 tgctgggcgg tgtcggaggc actttgcgcc agttgcgagg cctggcgggc gttatcggcg
     5881 ttttgcttca ctgtcgcggt gagctgctcc atgctggcgg cagtttcttc cagcgcggat
     5941 gcctgctgtt cagtacggga ggaaagatcg gtgttgcccg ccgcaatttc acgggtaccg
     6001 gcatagatgg catctgaacc ttcgcggaca tgagtgacgg tgtcagtcaa agagcgttgc
     6061 atatgtgaaa cgctctgcgc caggtcgccc atttcactgc gcccgtcaat ggtcagggta
     6121 ttcgctaggt taccaccggc gatttcgcga atgtgagcaa taatttttgc cagcggagta
     6181 agcaacatac ggcgaatgcc gtaccacgcc accagcagaa tcaataccac caccagcgcg
     6241 ataaccgcca gttgccactg ggcaaatcgg taatcatctg cgttgtcagt gacgatatcg
     6301 cgatacagtt tttcactgct gagggcgtac tgagcaaacg cttcgcccat tgcattttgc
     6361 attccctggg ttggctgagc gaaataagct ccagtattgc cataatctag ataatcaatc
     6421 agttcagtta acgctgtgta atagttttta tatttttcat caatattacg actggtagcg
     6481 accatttcag gtaacggtgc catgcttttg aattttttat aatgcgtcgc tgcctgcgcc
     6541 aatgttttcc tggcgctatc gagcaattca actttggcgt tactttgttg attggaggaa
     6601 tccatcatca tccgcaccgc tgaacgactc aggttaatgc gcgtttgcag cattaaatcc
     6661 caggttgacg tcagctcgcc ctgctgttcc cgcaattgat tggaaaccac aaagctcttc
     6721 tggctatggt gaagggaaga aaaaaacaga ctgccggaaa taagctgtaa cagggcgaat
     6781 acccccagca ccattaccaa cagcgtgact acgcggatac ggttaatcat aaggcacctt
     6841 cctgaaaaca agttgatctc gttatcggca agaagggggg aaactttatt gctgatgcca
     6901 cccgccgcga aattgaaata aaaaacccga tgcgcagatc atcgggttca tttcaattga
     6961 ggaaatcggg agaattacgc cacttctgac gccgcgctat ctaacagcgc catctcttcg
     7021 ctgttcagca gtttttcgat gttcaccaga atcaacatac ggtcgcccag tgcgcccagt
     7081 ccagtgagat attctgttga aagcgtcacg gcaaactctg gtgccggacg aatttgctcc
     7141 gccgtcaatg aaagcacgtc tgaaacgccg tcaaccacga tgccgaccac ccgctgtccg
     7201 agattcagga cgataactac cgtgttgtcg ttatagtcca catccacctg gctgaactta
     7261 attcgtaagt caacaatcgg cacaataacg ccgcgcagat tcgtgacgcc tttgataaac
     7321 gctggcgtgt tcgcaatccg tgttacctga tcgtagccac ggatctcctg cactttcagg
     7381 atatcaatac cgtactcttc atcaccaagg gtaaatacca gaaattcctg gcctgacggc
     7441 tcgctggcca gctttgttac attcgtcata ccggtcatat tgttaccttt ttactcattc
     7501 aggcggcggt gttcgccata cgttgttcgc ggtttatcgc ctgcaaggcg gagacatcaa
     7561 caatcagtgc cacgctgccg tcgccaagaa tggtcgcagc agaaatgccg gggactttgc
     7621 gatagttact ttcaaggttt ttaaccacaa cctggtgttg accaattaat tgatccacca
     7681 gcaaggcgta gcggcgaccg ccactttgta agatcacaac aattccctgg gtggcttcgg
     7741 ttttcgcgcc cgcgacgttg aacactttcc acagttcgac gatgggcaga tattcacccc
     7801 gcacttccag cacccgctcg ccgccggcca gtggatggag atcggcttca cggggttgca
     7861 gtgattccat aacagcattc agcggcagaa tgaaaacttc atccccaacg cgtacggaca
     7921 tgccgtcgag gatggccagc gtcagcggca gtaaaatgcg gatcgtagtg ccagtcccct
     7981 gcttcgactg gatttcgaca tgaccgccca tctcctggat attacgttta acgacgtcca
     8041 tgccgacgcc gcgcccggag acgtcggtga cctgctctgc catggagaag cctggtgcaa
     8101 atatcagcat cgcgacttcg tcgtcgctca tgttttcgct gacagtcaaa ccttgcgagg
     8161 ccgcttttgc cagaattcgc tcacggttta gccccgcccc atcgtcggtc acttcaatgc
     8221 aaatgttgcc gccctgatgt tcggcagaca gaattaaatt tccgacgctg tttttacctg
     8281 cggcgagccg tttttctggc agttcaatac cgtgatcgag gctattgcgt accaggtggg
     8341 tcagcgggtc gataatgcgt tctatcaggc ttttgtcgag ttcagtagaa ctgcccacca
     8401 gcgtcagttc tacctgcttg ccgagttttc ccgccagatc acgcaccagc cggggatagc
     8461 gactaaaaac atattccatc ggcatcatgc gaatcgacat cactgattcc tgcaaatcac
     8521 gggcgttacg ttgtaactgc cccatgctgg ttatcaaatc accatgatta accgggtcca
     8581 gttcgctgga acgctgggca agcatggact gggtgataac cagctcgccg acgaggttaa
     8641 ttaattgatc aaccttttct accgctacac ggatgctggt ggattcattg ctgcgcgtcg
     8701 ttttttcccg ctccacgcgg ccggttggcg cttgttcggc tgccagttta agcactggtg
     8761 gggtggatat ttttggcgag acttctactg tttcaaaggt aatctgatcg gcttcaatca
     8821 caaaacagag taccgctgtg atgtcatctt cggcgatgtc gcccggtaat attgccgaga
     8881 gcgaatccgc ccctttcacc acgtcagtta acgttgtcag atgtcccagt tcttcttcca
     8941 gcaggtcgac ttccccggcc ttcaggcgcg aaaggataat tcgtcgcggc gactgactgc
     9001 gactctgctc atcttgcggt tcacttttgg caaccacact taatcgggtc actgcggatg
     9061 gcgtttcgcc tttcgcttct aatgccagtt gacgcaaggc ctggcagata taatcgaagc
     9121 tggcggcatc cggctcttgc gactgtttat aagcgtcgag ctgttcttgc atgatgtcct
     9181 tcgtttccaa aaacagattg ataatgtcgg tgttgagttg catctcacct cgtctggctt
     9241 catcgagcag gttttccatc agatgcgtgg tttcctgcaa aacgctgaag ccaaaagttc
     9301 ctgcccctcc tttgatcgag tgggcagccc gaaagatggc attcaattgt tcggcatctg
     9361 gcgcttccgg ctgcaaaacc agcagatgct gctccatgtc agccaacagt tcgtccgctt
     9421 catcaaaaaa tgtctgataa aaatcgctta tatccatgct cacgctgtca cctcggttcg
     9481 gctgatggca ttgtgggaac actgacctgt ggtgcaacct caggtttttc cagggcgctt
     9541 actggctcat tctggctttc ggcgttttca tgcaaaatgg cctgttcggc ttgtttgttc
     9601 agtaccagca ggctgatgcg acggttgacg gcatcatcag gtccgcgatc gcttaagcgc
     9661 atcgttgccg ccatgccgac gacacgtaac actttgccgc tatccaaccc tccgaccatc
     9721 agttcgcggc gggatgcatt ggcccgatcg gcagaaagct cccagttgct atatcctttc
     9781 tcaccgctgg cgtaggggaa atcatcggta tgacctgaaa ggctaatacg gttgggaata
     9841 ccgttcagta caggcgcaat ggcacgcaga atgtcgcgca tatagggttc gacatcggca
     9901 ctgccggttc taaacatcgg gcgattctgg ctatcgatga tctgaatacg tagaccttcc
     9961 tggaccagat cgattttgag atggggacgt aacgcccgca gtttcggatc ggactctatc
    10021 aactggtcga gatcaccccg caatttccgc aatcgacttt gctccatgcg ttttttcaga
    10081 tcttcgatgt tcggctgctt attcacttcc ccctggcttt gggtgtaatc atcaccaccg
    10141 ccgggaattg ggctttcact attagaaatg cgatcgccgc ccgtaaccgc agtcgccagt
    10201 ggagtccgga agtactccgc aatctgaatc agctcttttg ggctggagat ggagatcagc
    10261 cacatcacca gaaaaaaggc catcatcgca gtcataaagt cggcataagc aatcttccac
    10321 gatccatgtg ctgccccgtg gcttttggct ttgcgtcgtt tgacgacaat aatcggatgc
    10381 gcttgattct tcatgcttcc tcggttgtcg tctgttgttg cggatttttc accgcacgca
    10441 catgctcttc cagttcaata aacgacggac gttcgctgga atagagcgtt ttgcgaccaa
    10501 actcaacggc gataggcggt gcgtaaccgt tcagattaga aagcagagtg actttgacgc
    10561 actgcatcat tttgctggtt tcggcgcttt tctgacgtaa aacactcgct aatggggaaa
    10621 taaatccgta agccaataaa atgccgagga aagtccccac catcgcatgt gcgataagcg
    10681 cacccagctc ggcggcagga cgatcggctg aacctaacgc gtgaacgccc cccattacag
    10741 ccgcgacaat accaaacgcc ggaagtgagt ccccgaccag cgccagactg ttcgccggga
    10801 cttctgcctc gctttcgtgc gtctcaatct cttcatccat cagggcttcg atttcgaagg
    10861 tgttcatatg accgctgata atcagacgca gataatcgac gataaaatca agcatgacgc
    10921 tatccgcgag gatgcgtggg tagctggcga agatctcgct ctcacgggga ttttcaatat
    10981 cacgttccag cgaaaacatc cccatctgcc gcgatttcgc catcaaccga taaagcagag
    11041 ccagcagatc catatacatt gctttggtgt atttggagcg acgaaacagc aacggcagcg
    11101 ccttcagcgt gcctttaatc gctttgccat tattgccgac gataaacgac ccaatccctg
    11161 caccggcaat aatcaccagt tcagcgggtt gatagagtgc tccaaggctt ccaccggtca
    11221 tcaaataacc gccgaaaact gtaccgagaa caaccaggta acctaataag ataagcacga
    11281 catcatcctt ccactgttga ccatgacagg atgttcagtc gtcaggcgtt aacgcgcgat
    11341 tggggcaaaa aaagcagcgg tacgtcgtta ccgctgctgg aatgttgcgc ctcaccgtat
    11401 cagttaaaca gcctgtactc tctgttcatc cagcagttgt gggataatat cggcaggatt
    11461 ctgggaaagt ttacgtcttt ttactgcccg ggatggcggt tgacataagc tgcaggcaaa
    11521 gctgccaaca ggctggtgag cgtgggtaat aaaattgccg ccgcagcagt tgcagctgga
    11581 aagttgcagt aatccacttt caacaaaccg caccaatgtc caggcacggg ttaatgccag
    11641 cagtggtcct tcttctgctt gtgggcactg ttcaaggtat aaacggtagg ctttgatcac
    11701 cgcatcgacg ccattacaca aaccggtttt cagtaaaaac tgccatgcat tacagaacat
    11761 cgaagcatga acgttttgtt cccaggtcat aaaccagtcg gttgagaatg gcagcatgcc
    11821 tttcggcggt gggcttccgc gcagttcttt ataaagtttt atcaggcgtc cgcgacttaa
    11881 ctgtgtttcg ctttccagca tctgcaaacg agcgcccagg gtaatcaatt ccattgccag
    11941 ctgaatatcc cgcgcttcct gaacaatgct tttttcactc atgatcaggc ccttttcttg
    12001 cgcagcgctt cttcaggctg attaacatca ttcagcaagc gtgttgagag catgatgccg
    12061 gtatgaattt gctggagatc gtcaacgcgg gaatcttgcg tcaactgagt aatcgtctgg
    12121 tggctgtcaa aacggaagtg acaaaccagt tgattggttt ctgccagctt aaccatttgc
    12181 ggaagagtca gtgccgctaa cgttgtcgcc atttcttcat ttatgccgag acgaaacata
    12241 gcggacgctt tgtcctgaac aatcaaacgc tgtgcaagta gtaaatatga caagttgatg
    12301 tcataaatgt gtttcagcaa ctcggaggta tgcattattc ccacccagaa taaccaactt
    12361 tatttttatg cggtttcacc gcaccccgtg atgtcgccgg gaagccccgg taaaaaataa
    12421 ttagcattag aatagttgcg ataagctgca ataagcagaa ccaccttttt ggtttaatat
    12481 gtccttacaa atagaaatgg gtctttacac ttatctaaga tttttcctaa atcgacgcaa
    12541 ctgtactcgt cactacacgc acatacaacg gaggggggct gcgattttca ataatgtgtg
    12601 atgcagatca cacaaaacac tcaattactt aacataaatg tataagtcat acttttgttt
    12661 tgggtgtatt tcaatatgtt aaaaagtttt tcgctacgct agcaagcaaa aatgaaacag
    12721 gaataatcga aatgggatgt tgcgcacagt taaaataaat caccgtaaat aatcatctgc
    12781 tataaataat cactttcatg caataccaga taagctattt ttaaacagac acttaccgca
    12841 caacaaacta attaaataca aagaaaggca acaatcaact taatgttaat ga
//
LOCUS       DAZPDU010000126        12563 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZPDU010000126 DAZPDU010000000
VERSION     DAZPDU010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12563
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.127"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..833)
                     /gene="gap"
                     /locus_tag="ACHURS_003151"
     CDS             complement(<1..833)
                     /gene="gap"
                     /locus_tag="ACHURS_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFR1238816.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IV"
     gene            1022..1552
                     /gene="cybB"
                     /locus_tag="ACHURS_003152"
     CDS             1022..1552
                     /gene="cybB"
                     /locus_tag="ACHURS_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFR1238817.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVMYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            1797..1970
                     /locus_tag="ACHURS_003153"
     CDS             1797..1970
                     /locus_tag="ACHURS_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1238818.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            1996..2064
                     /gene="yncP"
                     /locus_tag="ACHURS_003154"
     CDS             1996..2064
                     /gene="yncP"
                     /locus_tag="ACHURS_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFR1238819.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(2042..2191)
                     /gene="hokB"
                     /locus_tag="ACHURS_003155"
     CDS             complement(2042..2191)
                     /gene="hokB"
                     /locus_tag="ACHURS_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFR1238820.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            2590..4230
                     /gene="trg"
                     /locus_tag="ACHURS_003156"
     CDS             2590..4230
                     /gene="trg"
                     /locus_tag="ACHURS_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFR1238821.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASCIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMKDEPAGRNEIGRLNRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            complement(4268..5191)
                     /locus_tag="ACHURS_003157"
     CDS             complement(4268..5191)
                     /locus_tag="ACHURS_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR1238822.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            5408..6709
                     /locus_tag="ACHURS_003158"
     CDS             5408..6709
                     /locus_tag="ACHURS_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR1238823.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLY"
     gene            6976..8631
                     /gene="opgD"
                     /locus_tag="ACHURS_003159"
                     /gene_synonym="mdoD"
     CDS             6976..8631
                     /gene="opgD"
                     /locus_tag="ACHURS_003159"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFR1238824.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            8771..8995
                     /locus_tag="ACHURS_003160"
     CDS             8771..8995
                     /locus_tag="ACHURS_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFR1238825.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            9058..9594
                     /gene="rimL"
                     /locus_tag="ACHURS_003161"
     CDS             9058..9594
                     /gene="rimL"
                     /locus_tag="ACHURS_003161"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFR1238826.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(9589..10569)
                     /gene="ydcK"
                     /locus_tag="ACHURS_003162"
     CDS             complement(9589..10569)
                     /gene="ydcK"
                     /locus_tag="ACHURS_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFR1238827.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIRDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ISGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            10693..11685
                     /gene="tehA"
                     /locus_tag="ACHURS_003163"
     CDS             10693..11685
                     /gene="tehA"
                     /locus_tag="ACHURS_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFR1238828.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            11682..12275
                     /gene="tehB"
                     /locus_tag="ACHURS_003164"
     CDS             11682..12275
                     /gene="tehB"
                     /locus_tag="ACHURS_003164"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFR1238829.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
BASE COUNT         3208 a         2927 c         3146 g         3282 t
ORIGIN      
        1 actatttctt catcggtata accaaatgac tcgttattgg tggttgcttg tttaagtgcg
       61 ttattcacct cttcggcagt cacttttttt ccgagaatcg ataccagttc agtgaccgaa
      121 cctgttttca ccggcacgcg ttgcgcatga cctttcagtt tgccgctcag ttccgggatc
      181 accagaccaa tggcttttgc cgcccccgta gtgtggggaa tgatattttc tgccgctgcg
      241 cgtgaagcac gtaaatcttt accacgtggg ccatccacca gtgactgggt gccagtatag
      301 gcatgaatgg tcgtcatcgt gccgacttct atcccgaaac tgtcatgcaa ggctttggcc
      361 atcggcgcaa gacagttagt ggtgcatgac gccacggaaa caatggtgtc gttgccatcc
      421 agagtgtcgt cattgacgtt ataaacgata gttttcattt caccggcagg ggcggaaatc
      481 aacaccttct tcgcaccagc atcaagatgt gcctgcgatt tctcggcgga ggtataaaag
      541 ccagtacatt cgacaatgat ttctgcacct ttcgctttcc acggaatatt tttagcctct
      601 ttttcggcgt aaaccgcgat acttttccca tcaacgataa gtgaatcttc cgtaaaatca
      661 acgctccagg ggaatggtcc gtagtttgaa tcatgtttca gcaggtaggc gagaattttt
      721 ggggaagtga gatcattaat agcgacaacg tctatgttgc ttttgacttc aagtaatcga
      781 cgcaacacca gtcgaccgat acgaccaaaa ccgttaatac caactttact catggttttc
      841 tcctgtcagg aacattcgga tgaaaattga tcctttccag gcttagacca ggattgtggg
      901 atgggcaatc tccattctta cagtgaaacg taacgtactg aaaacgggtg aacaatattt
      961 aatgaaattt tgagaaaagc ccgttatgtt aacggaaaat tatgttaaag caggaaatgt
     1021 tatggaaaat aaatactcaa ggttacaaat cagcattcac tggctggtct ttttgctggt
     1081 tatcgcagcg tattgcgcaa tggagtttcg tggtttcttc ccacgtagcg atcggccact
     1141 catcaacatg attcatgttt cctgcggcat ctcaattctc gtgctgatgg tcgttcgtct
     1201 tttgttaagg ctgaaatacc caaccccgcc gattatacct aagccaaaac cgatgatgac
     1261 gggactggcg catttgggac atttggtgat gtatctcctg tttattgcgc tgccagtgat
     1321 tggtttggtg atgatgtata accggggcaa cccgtggttt gcgtttggtt tgacgatgcc
     1381 ttacgcttca gaggccaatt tcgaacgggt tgatagctta aagtcgtggc atgagacgct
     1441 ggcgaatctg ggatattttg tcatcgggtt gcacgctgcg gctgcactgg cacaccacta
     1501 tttctggaag gacaacacac ttctacgcat gatgccgcgt aaacgttcct gaaggatatt
     1561 tcaagaaaat gcctgtacta aaaccgaccc gtggtacagg cgaagaatac gggtctacat
     1621 cggaagcgcc tattatattt atttgtatga caaataaaac cataatcctt gcccatacgt
     1681 ccatctggct tatttttaat caattcaccc gatctttgat ctcatcaacg gtatcaaaat
     1741 aaaccagcgt ataacgttca aatcgaacat aagattcgat aaaccatgga ggttatatga
     1801 aaaaactggc acttattttg tttatgggaa cgcttgtttc cttttatgcc gatgccgggc
     1861 gcaaaccctg ttctggttcg aaagggggga tctcacactg tacggcaggc ggcaaatttg
     1921 tctgtaatga tggttctatt agtgcatcga aaaaaacatg cactaactga agtgtaaaag
     1981 gggtgccatg agaaaatgaa tctgctagtc agatgcgcgg ggaaaatccc cgcgcttgcc
     2041 cttacctgga cgtgcaggcc atgagcgcag caacctcctt atcaccgtcc cggaaccgca
     2101 gttcgtagag cgtttgtcgg gtcaggagtg tgaatgtcag aatcgtaatg cagataatga
     2161 gcagacacac taccagaggg ttgtgcttca tagcctttct ccttgccgga tggcgggtaa
     2221 gaggctaaga tctgaattgc taggttcatt cgttggcctc ggttgataga aatatcggtc
     2281 ggggccttcg tctttctgat tcccggttag cctgaaaaca gaaagtctca ggcacccgca
     2341 ggcatcctat gaggtttcct tagggacgaa aataatcact tcacgaaatt gcgtgctgtt
     2401 ttccagaatt tttcgtcatt cgggttagcc agtttagcca ttcgttactc tcttcattcc
     2461 aatagcatta attttctctg caataattgt tgtaaaaatg tgacgcaaag aggtttttgg
     2521 tcataagtaa ttaccgtcaa gtgccgatga ctttctatca ggagtaaacc tggacgagag
     2581 acaacggtaa tgaatacaac tccctcacag cgattaggtt ttttgcatca catcaggctg
     2641 gttccgttat ttgcctgcat tcttggcggt atcttagttc tattcgcatt aagttctgct
     2701 ctggctggct atttcctctg gcaggccgat cgcgatcagc gtgatgttac tgcggagatt
     2761 gagattcgga ccgggttagc gaacagttca gattttttgc gttcagcccg gatcaatatg
     2821 attcaggccg gggctgcgag ttgtattgcg gaaatggaag caatgaagcg aaatattgcg
     2881 caagccgaat cggagattaa acagtcgcag caagggtatc gtgcttatca gaatcggccg
     2941 gtgaaaacac ctgctgatga agccctcgac actgaattaa atcaacgctt tcaggcttat
     3001 atcacgggta tgcaacctat gttgaaatat gccaaaaatg gcatgtttga agcgattatc
     3061 aatcatgaaa gtgagcagat ccgaccgctg gataatgctt ataccgatat tttgaacaaa
     3121 gccgttaaga tacgtagcac cagagccaac caactggcgg aactggccca tcagcgcacc
     3181 cgcttgggtg ggatgttcat gattggcgcg tttgtgcttg ccctggtcat gacgctgata
     3241 acatttatgg tgctacgtcg gatcgtcatt cgtccactgc aacatgccgc acaacggatt
     3301 gaaaaaatcg ctagtggcga tctgacgatg aaggatgaac cggcgggtcg taatgaaatc
     3361 ggtcgcttaa atcgtcattt acagcaaatg cagcattcac tggggatgac agtagggact
     3421 gttcgacagg gtgcggaaga gatttatcgt ggcaccagcg aaatttcagc tggcaatgcg
     3481 gacctgtcat ctcgcaccga agaacaagcg gcggctatcg aacaaactgc cgccagcatg
     3541 gagcaactca ctgcgacggt gaaacagaat gcggataacg cgcatcatgc cagcaaactg
     3601 gcgcaagagg cttctattaa agccagcgat ggcgggcaga cggtttccgg tgtagtaaaa
     3661 acgatgggcg ctatctccac gagttcgaag aaaatttctg agatcaccgc cgtcatcaac
     3721 agtattgctt tccagacgaa tattctggca ctgaatgctg ccgttgaagc cgcgcgagcg
     3781 ggtgagcaag ggcgtggatt tgccgttgtc gccagcgaag tacggacact cgcaagtcgc
     3841 agcgctcagg cggcgaaaga gattgaaggc ttgatcagtg aatcagtcag gttaattgac
     3901 ctggggtcgg atgaggtggc aacggccggg aaaaccatga gcactattgt tgatgccgtc
     3961 gcgagtgtca cacatatcat gcaggaaatc gccgccgcct cggatgaaca aagtagaggc
     4021 ataacgcagg ttagccaggc gatttctgaa atggataagg tgacgcaaca gaatgcttct
     4081 ctggtagaag aggcctcagc ggcggcggtg tcccttgaag aacaggcggc acgattaact
     4141 gaggcggtgg atgtattccg tctgcacaaa cattctgtgt cggcagaacc tcgcggagcg
     4201 ggtgaaccag ttagtttcgc tacggtgtga aaatgttcaa ggagggatcg acagatccct
     4261 tcacctttca gaacggcatt gattttcgaa tagcgttaat catcaactgg caaccagaag
     4321 agaacgtcgc atctacgcgg gtcagtattc caatcggttc gcctgcacca tgtcccggaa
     4381 caggcagggc caccagcgtg gcatgacgca ggtcgtcttt tacagcgcca gaagggacaa
     4441 accacacgta atcgtattca accgtaagtt gacgagatag cgaagcagac agcgtttcga
     4501 tacaacccga aggaatttta cagccctggc tctgcactaa tgcatctgaa tgctggcgtg
     4561 gcgcagtgcc ttctggtgat acaacgaccg gccattccag cacccggctt agcgttacgt
     4621 tctcctgaag tagcgggtga ttagggcgga caaccagctt cagcgattca agaaacagca
     4681 gttcgtaatt aagcccggtc atcagttcag gatctgacat ccgaccaatg ccgatatcga
     4741 tttccccggt ttttaaaccc gccagaatca tagggttact cattgtcgca acttgcaagg
     4801 tcgtctcttt ttgttgctga tgaaactgac ctataaccga aggtaatatc cccagtgccg
     4861 cagtaggtag tgcaccaacc ctgacgacat cattattaag accttcttta cgatgaagcg
     4921 actgtccggc agtgttgatg gcgtcaagaa ctctgactgc atgcgttaaa aattgttcgc
     4981 cgggtaaggt aagttgcgcc ccctgacgac cacgctcaaa caagcgagcg cccgtcagct
     5041 gctccagttc attcaatgtc ttagagagcg caggttgact caaattaagg gtttcagccg
     5101 cgcgccccaa agttccttgt tgtgcgacag ctacgaatgt atgaaggtgg cgcaaacgga
     5161 tgcgctgact aaacagacta tttttttcca taagcgatgt taaaaacgaa gcggtgtcgc
     5221 tgacaagtga agttgtttga ttatgataac ttgattgcaa aatattatta acaattaaag
     5281 caattatgtt acagcaaaat ggataatatt gatgttttcg cggcgagatc acagtttgta
     5341 aattcttccc gccagagtga atgcggttac ctacactcca gattactgac cactggaggc
     5401 agacactatg gcgaacagca tcacggcgga tgagattcgg gaacagtttt cgcaggcaat
     5461 gtcagccatg taccagcaag aagttccgca atatggcacg ctgctggaac tggtagctga
     5521 tgtgaatctg gctgtgctgg aaaacaatcc tcaactgcac gaaaaaatgg taaatgcaga
     5581 cgagctggcg cgactgaatg ttgaacgtca tggggcgatt cgcgttggga ctgcacaaga
     5641 gcttgctact cttcggcgga tgtttgccat tatggggatg tacccggtga gctattacga
     5701 tctctcgcag gcaggggtgc cggtacattc gacagcattt cggcccattg atgatgcttc
     5761 tctggcgcgt aatcccttcc gcgtttttac ctccttactc cgccttgagc ttatcgagaa
     5821 cgaaattttg cgccagaaag cggcggagat tctgcgtcag cgcgatatct tcaccccacg
     5881 ttgtcgacaa ctgttagagg aatatgagca gcagggcggt tttaacgaaa cacaggcaca
     5941 ggagtttgtg caggaagccc tggaaacgtt tcgctggcac cagtcagcaa cggtagatga
     6001 agaaacctat cgcgccttgc acaacgaaca tcggttgatt gctgatgtgg tctgttttcc
     6061 tggatgccat atcaaccacc tgacgccacg tacgctggat attgaccggg tgcagtcgat
     6121 gatgcctgaa tgcggaattg aacccaaaat tctcatcgag gggccgccgc gccgcgaggt
     6181 accgatttta ctacgccaga ccagctttaa agcactggaa gagacggtgt tgtttgcggg
     6241 gcagaaacag ggcacgcata ccgcgcgctt tggtgaaatt gagcagcgtg gcgtggcatt
     6301 aacgccgaaa gggcgacaac tgtatgatga tcttctgcgt aacgctggaa ccgggcagga
     6361 taatctcact caccaaatgc atttacagga aaccttccgc acttttcctg acagtgagtt
     6421 tttaatgcgt cagcaagggt tggcatggtt ccggtaccgt ctgacgcctt cgggtgaggc
     6481 gcatcgtcag gcgattcatc ctggagacga tccacagccc ttaattgaac gtggttgggt
     6541 agtggcgcaa cccatcacct atgaagattt cttgcccgtt agcgcggcgg ggatcttcca
     6601 gtcaaatctg ggtaatgaaa cgcagacacg cagtcacggt aatgccagtc gcgaagcatt
     6661 tgagcaggcg ttgggttgtc cggttttgga tgagttccag ctttactagg aagcggaaga
     6721 acgcagtaaa cgtcgctgtg gtttgcttta aattctgacc atccgccttt gcaaaaattt
     6781 gcctgatttt acaaacgaat caggctcatc ccatcgacat aaaaaaaatg ccgatttatg
     6841 catattctct cagttcaaca attggattat taataaatat tgtctagagt gagcggtcat
     6901 aaataagcac tttcttgccg ctgaaaacga ccagcgcggg accattcaca acaccagaag
     6961 gactcacttt caggtatgga tcgtagacga tttattaaag gttcaatggc tatggccgcc
     7021 gtgtgcggta ccagcggcat tgcttctctt ttttctcagg cggcattcgc ggcagattct
     7081 gatattgccg acgggcaaac ccagcgtttt gacttctcca ttctacagtc aatggcgcac
     7141 gacttagcgc aaacagcgtg gcgtggtgcg cctcgtccgt tacctgacac gctggcgaca
     7201 atgacgccgc aggcttataa cagtattcaa tacgacgccg aaaaatcgct ctggcataac
     7261 gttgagaacc gtcaactgga cgctcagttc ttccatatgg gaatgggatt ccgtcgccgc
     7321 gttcgtatgt tttctgtaga tccagcaaca catctggcgc gtgaaattca ctttcgcccg
     7381 gagttgttca aatacaacga tgcaggtgtt gataccaaac aattagaagg gcaaagcgat
     7441 ctcggctttg ccggttttcg cgtgtttaaa gcccccgaac tggcgcgccg tgatgtagta
     7501 tcatttctcg gcgcgagtta tttccgcgcc gttgatgata catatcaata cggtttgtcg
     7561 gcccgcggcc tggcgatcga cacttacacc gacagtaaag aagagttccc cgactttacc
     7621 gccttctggt ttgatacggt aaaaccgggg gcaactacct ttaccgttta tgcgttgctc
     7681 gatagcgcca gtattactgg tgcctataag ttcactatcc attgtgagaa aagtcaggtg
     7741 attatggatg tggaaaatca cctgtatgcg cgcaaagaca ttaaacagct gggcattgcg
     7801 ccgatgacca gtatgttcag ctgcggtact aatgaacgtc ggatgtgcga caccattcat
     7861 ccgcaaattc atgactctga tcgtctgtcc atgtggcggg gcaacggcga gtggatttgc
     7921 cgtccgctga acaatccgca aaaattgcag ttcaatgctt acaccgacaa caacccgaaa
     7981 gggtttggtt tattgcaact ggatcgtgac ttctcccatt atcaggacat tatgggctgg
     8041 tataacaaac gcccaagttt gtgggtggaa ccgcgtaaca agtggggtaa gggcaccatc
     8101 ggcctgatgg aaatcccaac aacgggcgaa acgctggata acattgtctg cttctggcag
     8161 ccagaaaaag ctgtaaaagc aggtgatgag tttgcattcc agtatcgtct gtactggagt
     8221 gcgcaaccgc ctgttcattg cccattagcg cgcgttatgg cgacgcgtac cggcatgggc
     8281 ggtttcccgg aaggttgggc gccaggtgaa cactatcccg aaaaatgggc gcgtcgtttt
     8341 gccgtcgatt tcgttggtgg tgatctgaaa gctgccgcgc caaaaggcat tgagccggtg
     8401 attacgcttt ccagtgggga agcgaagcaa atcgaaattc tctatattga acccatcgat
     8461 ggttatcgta ttcagtttga ctggtatccg acttcggact ctactgatcc ggtcgatatg
     8521 cggatgtatc tacgttgtca gggggacgct atcagtgaaa catggctgta tcagtatttc
     8581 ccgccagcgc cggataaacg tcagtatgtt gacgaccgcg tgatgagtta atcgtttttt
     8641 cttcggcacc ttcttcggga ggtgccgtct ggttaaacac gatcccgctc gcatttttcc
     8701 ctaagttaaa tgagtaatct gatggtgtgt atttcagata caccttgtca gccactaaca
     8761 gggagtgcgt atgtttccag aataccgaga tttaatatcc cgtctgaaaa acgaaaatcc
     8821 tcgctttatg tccttgttcg ataaacacaa taaacttgat catgaaattg ccagaaagga
     8881 aggttccgac ggtcgagggt acaatgcgga agtggtccgc atgaaaaaac aaaagctaca
     8941 gttaaaagat gagatgctca aaatcctgca gcaggagagc gtcaaagagg tgtaaacttt
     9001 cctaagccgc cagccaggcg gcttttttaa caacagcatg gattgactgg agataagatg
     9061 actgaaacga taaaagtaag cgaatcactt gaattacatg ctgttgcgga aagtcacgtc
     9121 acacctcttt atcagttaat ctgtaaaaat aaaacctggt tacagcagtc gctaaactgg
     9181 ccgcagttcg ttcagagtga agaggacacg cgaaaaacgg tgcagggtaa tgtgatgttg
     9241 catcaacgcg gctatgccaa aatgttcatg attttcaaag aagatgaact tatcggcgtt
     9301 atctcgttta atcgtattga accactgaat aaaactgctg aaatcggcta ctggctggac
     9361 gaatctcatc aggggcaggg gatcatttct caggcgctgc aggcattgat tcatcattac
     9421 gctcagtctg gtgaacttag acgcttcgtg atcaaatgtc gggtggacaa tccgcaaagc
     9481 aaccaggtcg ctttgcgcaa tggttttatc cttgaaggtt gcctgaaaca ggctgagttc
     9541 ctgaatgatg cctatgatga tgtgaactta tacgcgcgta ttatcgattc ataataaccc
     9601 tgccagcggc gttcgcgtaa tgcgctcctc gccgttaatg acttttggac cacggacatg
     9661 aaccgtgtcg ccatcaaagg cttcaacgac ggcgtggtcg gttaactcca catggttttc
     9721 aattatcacc gcacctgaaa tacggctctc accttgtatt acaacgtgct cgtcgagcag
     9781 aatcggtcca ccacgtacca cggcatttcc gccaatcaag acatgatgtt ttaacacaca
     9841 attaccttcc acaatggcat attccgccac ctgcgaactg taatgaatcg tcggaatggc
     9901 atcttcttct atgccagctt tcacctgcgc gtgaccgtag actttagcgc aatcgcatag
     9961 ccagacgttg ttctcttcat taccttcaac actggcaaaa tcaaaaactt cggcgcgatg
    10021 ttcaataaaa gcataccgga cgacggcatc gccataaatt tgtgcctggt ggacaatacg
    10081 cgaggcgcta acccttgcgc gatcataaat ttggagcagg agttgatggt cgggcgttaa
    10141 gccttgtgcg gcgacgatca tagaatgttg atcaattaag gcgtgaccaa atattctaca
    10201 ttgcccgtaa actaatgagt cacgaatggt gacactgtca cttatatatg caccctggct
    10261 gatttcacta ttatcgatcc agacgttatc cgttgcgtaa acctctcccc acagcacgct
    10321 ggtgccggtg atacgcgtat taccagaaat caccgttccg ccgaacgcaa tagcattctg
    10381 gtcgtaaatc cagcaatttc cttcctgtgc taacaccgtt tcgcgatcga tccagccgcc
    10441 agcggttcct gctatcacat cgttaaaatc gctaatggcg ataatctgcc gtaataacac
    10501 acttttttta gtgccatctt cctgataact aaaggctcgc tgttcttcac ttagacgata
    10561 tttgcgcata gcgttttctc acaggtgact tactataacc gtagcaaatt ctgcggctct
    10621 ggctatgctc gagaaaattc cataaaatgc atttcaaata tactttataa attaaacaaa
    10681 atgagtaaga agatgcagag cgataaagtg ctcaatttgc cggcaggcta ctttggtatt
    10741 gtgttgggga cgatagggat gggatttgcc tggcgctatg ccagccaggt ttggcaggtc
    10801 agccactggt taggggatgg gctggtgatt ctggcgatga tcatctgggg attattaact
    10861 agcgcattta ttacccgact catacgcttt ccgcatagcg tgctggcgga agttcgccat
    10921 ccagtgctga gcagttttgt gagtttgttt ccggcaacga cgatgctggt ggcgattggt
    10981 tttgttccgt ggtttcgccc actggcggtg tgcctgttca gttttggtgt cgtggttcag
    11041 ttggcttatg ccgcctggca aactgcggga ttatggcgcg gatctcaccc tgaagaagct
    11101 accacgcctg gactgtatct gccgacagtt gccaacaact ttatcagcgc aatggcctgt
    11161 ggtgcgttgg gctacaccga cgccggtctg gtgtttttag gcgcaggcgt tttctcatgg
    11221 ctaagtcttg aaccggtgat cttgcagcgt ctgcgcagtt cgggagaatt acccacggca
    11281 ctgcggacat cactcggcat tcagctcgct cctgcgctgg tggcctgtag tgcctggttg
    11341 agcgtcaacg gcggcgaggg tgatacgctg gcgaaaatgc tttttggtta tggactgctg
    11401 caactgctgt ttatgctacg tctgatgcca tggtatctct cccagccatt taatgcttca
    11461 ttctggagtt tctcgttcgg cgtatctgca ctggcaacca ccggtttgca tctggggagt
    11521 ggcagcgaaa atggattttt ccatacgctg gcggtaccgc tgtttatctt taccaatttt
    11581 attattgcaa tactgctcat ccgtactttt gcgcttctga tgcagggaaa attgttagtc
    11641 agaaccgagc gcgccgtttt aatgaaagca gaggacaaag aatgatcatt cgtgacgaaa
    11701 actattttac tgataaatat gaattaaccc gcacacactc tgaagtactg gaagcggtga
    11761 aagtggttaa accgggtaaa acgctggatc tgggctgtgg caatggtcgt aacagtcttt
    11821 acctggcagc caatggttat gatgttgacg catgggataa aaatgccatg agtatcgcca
    11881 atgtagagcg cattaaatcc attgaaaatc tggataattt acacacccga gtcgttgatc
    11941 tgaataacct cacatttgat agacagtacg attttattct ttcgactgtg gtactgatgt
    12001 tccttgaggc taaaaccatc ccagggctga ttgccaatat gcaacgttgc actaaacctg
    12061 gtggttacaa cctgattgtg gcggcgatgg ataccgctga ttatccatgt accgtcggct
    12121 tcccgtttgc cttcaaagag ggagaattac gtcgatatta cgaaggctgg gagagggtga
    12181 aatacaatga agacgtcggc gagctgcacc gcaccgacgc caacggtaat cgtattaaac
    12241 tgcgtttcgc cacgatgctg gcacgtaaaa aatgacccgc taagaataag gcgcgtgaaa
    12301 attccccacg ctgagatgat ttactgttct tcttttcggt aagcatattt tttatcgaag
    12361 ggatgtgaaa ttaatcacag tagtcgaagt tttttagcag cttaacttac tgaaatttaa
    12421 ctactgatga ttgacttagc ccctttttcg gcattgacta tgtcgtctga aaaggggctg
    12481 aaaaatttat tttcaccaac actttttttg ccacaacacg aagcggcgct ttttgctata
    12541 acttaaaaag taatataatc atc
//
LOCUS       DAZPDU010000127        12239 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZPDU010000127 DAZPDU010000000
VERSION     DAZPDU010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12239
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.128"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..323
                     /locus_tag="ACHURS_003165"
     CDS             <1..323
                     /locus_tag="ACHURS_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1238830.1"
                     /translation="ASMLPPLFIMLAYLNLRAKLDHLPRDFRMGSRRTGIIVVSMLIA
                     IFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFAWWKYSKYIKGLTAEERHIEATPA
                     SNVD"
     gene            457..1534
                     /gene="ygjJ"
                     /locus_tag="ACHURS_003166"
                     /pseudo
     CDS             457..1534
                     /gene="ygjJ"
                     /locus_tag="ACHURS_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            1551..3902
                     /gene="ygjK"
                     /locus_tag="ACHURS_003167"
     CDS             1551..3902
                     /gene="ygjK"
                     /locus_tag="ACHURS_003167"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFR1238831.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWLSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGLYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            4128..6146
                     /gene="fadH"
                     /locus_tag="ACHURS_003168"
     CDS             4128..6146
                     /gene="fadH"
                     /locus_tag="ACHURS_003168"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFR1238832.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLNVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(6191..6607)
                     /gene="higA"
                     /locus_tag="ACHURS_003169"
     CDS             complement(6191..6607)
                     /gene="higA"
                     /locus_tag="ACHURS_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFR1238833.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLADLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(6604..6918)
                     /gene="higB"
                     /locus_tag="ACHURS_003170"
     CDS             complement(6604..6918)
                     /gene="higB"
                     /locus_tag="ACHURS_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HFR1238834.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            complement(7203..8339)
                     /gene="rlmG"
                     /locus_tag="ACHURS_003171"
     CDS             complement(7203..8339)
                     /gene="rlmG"
                     /locus_tag="ACHURS_003171"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFR1238835.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            8424..8927
                     /locus_tag="ACHURS_003172"
     CDS             8424..8927
                     /locus_tag="ACHURS_003172"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFR1238836.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPITKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            9004..9687
                     /locus_tag="ACHURS_003173"
     CDS             9004..9687
                     /locus_tag="ACHURS_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFR1238837.1"
                     /translation="MQPRLLLRICFSRRTLKITCLLLLVAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHVGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            9766..10752
                     /locus_tag="ACHURS_003174"
     CDS             9766..10752
                     /locus_tag="ACHURS_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR1238838.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLESEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            11035..12000
                     /gene="alx"
                     /locus_tag="ACHURS_003175"
     CDS             11035..12000
                     /gene="alx"
                     /locus_tag="ACHURS_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFR1238839.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
BASE COUNT         2908 a         3148 c         3290 g         2893 t
ORIGIN      
        1 cagcgtccat gcttccgccg ttattcatca tgctggctta cctgaattta cgcgccaaat
       61 tagatcacct gccacgcgat ttccgtatgg gctcccgccg caccggtatt atcgttgttt
      121 caatgctgat tgcgatattt gccgtagggt ttgtcgcttc gacattcccg actggcgcga
      181 atattctgac catcattttt tataacgtcg gcggtattgt tatcttcctt ggctttgcgt
      241 ggtggaaata cagtaaatat ataaagggat taacggctga agagcgccat attgaagcga
      301 cgccagccag caatgttgat taagcagaat aataaaaaat gagcaggcat tttccctctt
      361 cctggatgag gagagggaaa atagtttctg cctttatatt tttataatta caacgataaa
      421 aggctgtact ttttctttag ctcatggatt aacacaatga aattaatcac tgcaccatgc
      481 agagcattac ttgctctgcc gttttgctac gccttttctg cggcaggaga agaagcacgt
      541 ccggcagaac atgacgacac aaaaacaccc gcaattacct cgacatcttc tccttcattt
      601 cgtttttacg gcgaattagg gattaggggg tggtggatat atggatttag agggtgagaa
      661 taaacataaa tacagcgacg gtacctatat tgaaggtggc ctggagatga agtacggctc
      721 ctggttcggc ctgatttacg gcgaaggctg gaccgtgcag gccgaccacg acggcaatgc
      781 ctgggtgcca gaccatagct ggggtggttt cgagggcgga attaaccgtt tctatggcgg
      841 ttatcgtacc aatgatggca ccgaaatcat gctcagtctg cgtcaggatt cctcgctgga
      901 tgacctgcaa tggtggggcg atttcacccc cgatctgggc tacgtcattc ccaatacccg
      961 cgacattatg actgcgctga aggtacagaa cttaagcggc aactttcgtt atagcgtcac
     1021 cgcgactcct gccggacatc atgatgaaag caaagcctgg ctacattttg gcaaatacga
     1081 tcgctatgac gacaaataca cctatccggc aatgatgaac ggttacatcc agtatgacct
     1141 tgccgaaggc atcacctgga tgaacggtct ggaaatcacc gacggcacag gacagctcta
     1201 tctcacgggc ctgctaactc ctaactttgc cgctcgcgcc tggcaccata ccggacgcgc
     1261 cgacgggctg gacgtaccgg gaagtgaaag tgggatgatg gtgagcgcca tgtatgaagc
     1321 gttaaagggc gtttatctct ccaccgctta cacctacgcc aaacatcgcc ctgaccacgc
     1381 tgacgatgaa accacctctt tcatgcagtt tggtatctgg tacgaatacg gcggcggacg
     1441 tttcgccacg gcttttgata gccgcttcta catgaaaaat gcctctcacg atcccagcga
     1501 ccaaatcttc ctgatgcaat atttctactg gtaataagga ctgtaatttc atgaaaataa
     1561 aaactatttt aacgccagta acctgcgctc tgctgataag tttttccgcc catgccgcta
     1621 acgccgacaa ttataaaaac gtgattaacc gtactggcgc gccgcagtac atgaaggatt
     1681 acgattacga cgatcaccag cgttttaatc cgtttttcga tctcggagcc tggcatggtc
     1741 atctgttgcc tgacggccct aacaccatgg gcggctttcc gggcgttgcg ctgctgacgg
     1801 aagagtacat caactttatg gccagcaatt tcgaccgcct gaccgtctgg caggacggca
     1861 agaaagtcga ctttacgctg gaggcataca gtattcccgg tgcgctggtg caaaaactga
     1921 cagcaaaaga tgtgcaagtc gaaatgactc tgcgcttcgc cacgccgcgc acgtcactac
     1981 tggaaaccaa aatcaccagc aataaaccgc tggatctggt gtgggacggc gaactgctgg
     2041 aaaaactgga agcgaaagaa gggaaaccgc tttccgataa aaccattgct ggcgaatacc
     2101 ctgactatca gcgcaaaatc agcgccaccc gtgatggcct gaaagtcacc tttggcaaag
     2161 tgcgcgccac ctgggatctg ctgacctccg gcgaatcgga atatcaggtg cataaatccc
     2221 tgccggtgca gactgaaatc aacggtaatc gctttaccag taaggcgcat atcaacggtt
     2281 cgaccacgct ctacaccacc tattcccatc tgctgaccgc tcaggaagtt agcaaagagc
     2341 aaatgcagat ccgcgatatt ctggcgcgtc cggcgtttta tctcaccgcc tcgcagcaac
     2401 gctgggaaga atacctgaag aaagggttaa ccaatccgga tgcgacgccg gaacagacgc
     2461 gcgtcgcggt gaaagcgatc gaaacgctca acggtaactg gctctcaccg ggcggtgcgg
     2521 tgaaatttaa caccgttaca ccgtcggtga ccgggcgctg gttctccggc aatcagacct
     2581 ggccgtggga tacctggaag caggcgtttg cgatggcgca tttcaatccg gacatcgcca
     2641 aagagaatat ccgcgcggtc ttctcctggc agatccagcc tggcgatagc gtgcgtccgc
     2701 aggatgtggg ctttgtcccc gacctgatag cgtggaacct tagccccgag cgtggtggcg
     2761 atggcggcaa ctggaacgaa cgtaatacca aacccagcct tgccgcctgg tcggtgatgg
     2821 aagtgtacaa cgtcacccag gataaagcct ggttggcaga gatgtacccg aaactggtgg
     2881 cctatcacga ctggtggtta cgtaaccgcg atcacaacgg caacggcgtg ccggaatatg
     2941 gcgcgacccg cgacaaagcc cacaacactg agagcggcga gatgctgttt acggtgaaaa
     3001 aaggcgataa agaagagacg cagtctgggc tgaacaacta cgcccgcgtg gtggagaaag
     3061 gcctgtacga cagtctggaa attccggcac aggttgctgc atcgtgggaa tcggggcgtg
     3121 atgatgccgc cgtctttggg tttatcgaca aagaacagct ggataaatat gtcgctaatg
     3181 gcggcaaacg tagcgactgg acggtgaaat tcgccgaaaa ccgcagtcag gacggaacgt
     3241 tgctgggcta ctcgctattg caggagtcgg tggatcaggc cagctatatg tacagcgata
     3301 accattatct ggcggagatg gcaaccatcc tcggtaagcc agaagaggcc aaacgctatc
     3361 gccagttggc acagcagctc gcggactaca tcaacacctg tatgttcgac ccgactaccc
     3421 agttctacta tgacgtacgt attgaagata aaccactggc gaacggctgc gcgggcaaac
     3481 cgattgttga gcgcggtaaa gggccggaag gctggtcgcc gctgtttaac ggtgcggcaa
     3541 cgcaggccaa cgccgacgcg gtggtgaagg tgatgctcga tcctaaagag ttcaacacct
     3601 ttgtcccgct gggaacggcg gcgttaacca atccggcttt tggcgctgat atctactggc
     3661 gtgggcgcgt atgggtggat cagttctggt ttggtctgaa agggatggag cgttacggtt
     3721 atcgtgatga tgccctgaag ctggcggata cgttcttccg gcacgccaaa ggattaaccg
     3781 ccgatggccc aattcaggag aattacaacc cgctgactgg cgcacagcaa ggcgcaccaa
     3841 atttctcctg gagtgccgct catttgtata tgttgtataa cgattttttc cgtaagcagt
     3901 aatttgcaac ggctggcgga tgcggcgtga acgccttatc cgccctacat gtgcaatccc
     3961 ccaggccgga taaaacgcgg caagcgttgc atccggcgtt accaccgggc gtattctttt
     4021 tgaatcccat cacaaacccc gcactcccct tttccctttt ctccatcgac ggctaaatta
     4081 gaactcatcc gaccacataa caattatttt acatactgga caattttatg agctacccgt
     4141 cgctgttcgc cccgctggat ttaggtttta ccacgttaaa aaaccgcgtg ttgatgggct
     4201 caatgcacac cgggctggag gaatacccgg acggtgccga gcggctggca gcgttttatg
     4261 ccgaacgcgc ccgtcacggc gtggcgctga ttgtcagcgg cggcattgca ccagatttaa
     4321 caggcgttgg catggaaggt ggcgcaatgc tcaacgacgc cagccagatc ccacaccatc
     4381 gcaccattac cgaagcggta catcaggaag gcggcaaaat agccctgcaa attttgcata
     4441 ccgggcgcta cagctaccaa ccgcatctgg tcgctccctc cgcattgcag gcccccatca
     4501 accgtttcgt tcctcacgaa ctcacccatg aagagatcct gcaactgatc gacgatttcg
     4561 cccactgcgc gcaactggca cgggaggcag gatacgacgg tgtagaggta atggggtccg
     4621 aagggtattt gatcaacgaa tttctgacgc tgcgcaccaa tcagcgtagt gaccagtggg
     4681 gcggcgatta ccgcaaccgg atgcgatttg ccgtagaagt agtgcgtgcg gtgcgcgaac
     4741 gcgtcggcaa cgacttcatt attatctacc gactgtcgat gctcgacctg gtcgaagacg
     4801 gcgggacttt tgccgaaacg gtagagctgg cgcaggccat tgaagcggcg ggcgcgacca
     4861 ttatcaacac cggcattggc tggcatgaag cacgtattcc gaccattgcc acgcccgtgc
     4921 cgcgcggcgc atttagctgg gtcacgcgca aactgaaagg ccacgtctcg ctgccgctgg
     4981 taaccaccaa ccggattaac gatccgcagg ttgccgacga tattctctcg cgcggcgatg
     5041 ccgatatggt atcgatggca cgaccgtttc ttgctgatgc ggaactgctg tcaaaagcgc
     5101 aatcggggcg agccgatgag atcaacacct gcattggctg caatcaggcc tgcctcgatc
     5161 agatcttcgt tggcaaagtc acctcgtgcc tggtgaatcc tcgcgcctgc cacgaaacca
     5221 aaatgccaat ccttcctgcc gtgcagaaaa aaaatctggc ggtggtcggt gcgggacctg
     5281 ctgggctggc gtttgccatt aacgcggcgg cgcgtgggca tcaggtaaca ttgtttgatg
     5341 cccacagcga gatcggcggg cagtttaata tcgccaaaca gatccccggc aaagaggagt
     5401 tttacgaaac gctgcgctat taccaccgga tgatcgaagt gacgggcgtg acgctgaaac
     5461 tcaatcacac cgtgacggcg gatcagttac aggcgttcga tgaaacgatc ctcgccagtg
     5521 ggatcgtgcc gcgcgttccg cctatcgacg ggatcgatca tccgaaggta ttgagttatc
     5581 tcaatgtact gcgcgacaaa gcgccggtag gcaacaaggt tgccatcatc ggttgtggcg
     5641 ggattggttt tgatacggcg atgtatttaa gtcagccggg cgaatccacc agccagaata
     5701 tcgccaggtt ctgtaatgag tgggggatcg acagcagcct acaacaggct ggtggcttaa
     5761 gcccgcaggg aatgcagatc ccgcgtagcc cacggcaaat tgtgatgctc cagcgcaaag
     5821 ccagcaaacc gggacagggg ttaggcaaaa ccaccggctg gatccatcgc accaccctgc
     5881 tctcgcgggg tgtgaaaatg atcccaggcg taagttatca gaagattgac gatgacgggc
     5941 tgcatgtggt gatcaacggc gaaacgcagg tattagcagt ggacaatgtg gtgatctgcg
     6001 cagggcaaga gccaaaccgc gcgctggcgc aaccgctgat tgatagcggg aaaactgtgc
     6061 atttaattgg cggctgcgat gtggctatgg agctggacgc acgacgggca attgcccagg
     6121 gaacacggct ggcgctggag atttaaatcg ttattttaag cgccggatgc aacgcatccg
     6181 gcacgtcgcg ttaatcaata aacaaggcgg gagaaatgcc gaatcgcgtt gccaattttt
     6241 tagcgtgttc cagcgttaat ttcctcttcc cgctcaaaac gcgtgacacc atagatttac
     6301 tgccaatttc cggcagatcg gcaagggtta aaccatattg atccataagg gtacgaatca
     6361 cggctatacc gccaggcatg gcttgagcca tggcattaaa ttccgcaaat tcgggcgctg
     6421 attcttccca cgcggttatt ttggcacaca ccagatccag caaggggttt tcaggatcgt
     6481 tgagcagcag atgatctacc agttccagcg cctgggtgta ttgttcctcg ttctgaatac
     6541 ccgccagaaa aggtgccacc gcagttagct tttctcctgc ttgcaagatg tcggcaatcg
     6601 caatcatttt ttccccttag tacgatgaac agcggtaaag aaatcgtatt ctttatgcgt
     6661 cataacttca cgtatgtagc acttttgcga ttcaaaaaag accattgcta caacacgtaa
     6721 ttcattgccc ccaacattga aaacataatg cttatccaga tatttgaagt tatccagaga
     6781 tgggaatact gcttttaatg actcaggttt tttgaaatat cccttagcaa tcgtgttccc
     6841 cagagccacc aactccgttt tatgttgcgg gtatttttcc gcagcatctt tcaatgcttt
     6901 ttgagttatc aggtgcattc ttcatcacgt ccgttgacaa attggcaata tgataacatc
     6961 cgttgacaga ttggcaacgg atgaatttat ctgtgtgacg gaagaataaa cgtagaaaaa
     7021 cgctaaacaa gagaatgtag aggatggtga gagggatttt ttgttttatg gaagcgcgat
     7081 tccagaactg ggcagacatt gccggatgcg acgcttgccg cgtcttatcc ggcctgggag
     7141 taacgtgctg aacgtaggcc ggataaggcg ttaacgccgc atccggcagg taactcccaa
     7201 tcttaccgac gacgccccag cttcactgct ttcagcacca caaatttatt attcgtggcg
     7261 atggtggtgc agttgccgaa aatcttcttc agtttatgga agtaatccag gtgacggttg
     7321 gcaacaatat acagctcgcc gttgattttc aggcagcggc gggcatggtg gaacatctcc
     7381 caggcgacgt tatcggtcag cgcatgttgt tggtgaaacg gcgggttgca gagcacagca
     7441 ttaaagcgga aaggctccac gccggagagc gcgttgttga tcataaactc gctgcgatct
     7501 aacgcctctg gcatgttggt ttcaacgttc aaacggctgg aagcaaccgc catcggcgat
     7561 tcatcgacaa acaccacttt cgcctgaggg tttttatcaa gcagcgtcag accaataacg
     7621 ccattaccgc aaccgagatc gacaatctca ccttcgagat tctctggcag atgttgcata
     7681 aagaagcgtg cgccaatatc cagcccggtg cgggagaaga cattcgcatg gttgtggata
     7741 gtccagtcag taccttccag cttccagcta acggtctgcg gcgcatcggc cagcggcggt
     7801 tcattgaaag tgcaattaat caggcgcgct tttttccatg ccagcgtagt ggtggtcggg
     7861 ccgagcactt tctcgaacag ctccagcgtg gaagtgtgaa tgtcacgggc tttggcaccg
     7921 gcaataatac gtgtatccga cgtgaccact ttgcgcagcg cacgcagttg ctgttccagc
     7981 aatgccagtg ttttcggcac tttgatcagc accacacccg gctgttgcgg gtagtcggcg
     8041 gtgctgtcga gaaacttcac gctcgactca tcaatcccgt tgaggcgtaa attctcgcgc
     8101 gtcgccagtt cactgatgta tgagtcgcca atgctgtacg gcttatgttc cgccagcgcg
     8161 cagcttaacg caccaaaggc atcattcagg atcaacaccg ggccgcggat ttctgtgtcg
     8221 tccaactgtt gcagcaaata ttcatccgcc gcttcccacg cctgtagcgg gttaacgtca
     8281 tccgtcgccg gaaaacgttg tagtgtcagt gaacggaaac cgttgtctaa gtggctcatc
     8341 ggccctcctg aatgataaaa tttcgacgtt gcccctgaaa agggtgcgtg agtataccct
     8401 ttttctcttt ttcgtgggcg gttatgagca atcttactta tcttcagggt tatcccgagc
     8461 agctactttc ccaggtgcga acgctgatta acgaacagcg tctgggcgat gtgctggcaa
     8521 aacgctatcc gggaacacac gactacgcca ccgataaagc cctctggcaa tatacccagg
     8581 atctaaaaaa tcagtttctg cgcaatgccc cgccgatcac taaagtgatg tatgacaata
     8641 agatccacgt actgaaaaac gcgctcggat tacataccgc tgtttctcgt gtgcagggcg
     8701 gcaagctgaa agcaaaagca gagatccgcg tcgctactgt gtttcgcaac gcgccggaac
     8761 cgtttttacg catgatcgtg gtgcacgaac tggcgcacct gaaggagaaa gagcataaca
     8821 aagcgtttta ccagttgtgc tgccatatgg agccgcagta ccaccagctt gagttcgaca
     8881 cccggctgtg gctaacgcag ttgtcgcttg ggcaggacaa aatctgaaaa tgatgtcagg
     8941 cgttatcttt tctttcttta gcgcctaaaa tcgacctccc ccctttcgtt aaggataacg
     9001 accatgcagc cccgccttct tctccgtatt tgtttttctc gccggactct aaagattacc
     9061 tgcttactgt tacttgtggc aggagcgacc atttttatcg ccgatcgcgt aatggtcaac
     9121 gccagcaagc agctgacctg gagcgatgtc aacgcagttc cggcgcgtaa cgtgggttta
     9181 ttgctggggg cgaggccggg taatcgctac tttacccgac gtattgatac cgcagcagcg
     9241 ttgtaccacg tcggaaaagt gaaatggctg ttagtcagtg gcgataacgg acgtaaaaat
     9301 tacgatgaag cctcaggtat gcagcaggct ttgatcgcta aaggcgtacc ggcgaaagtg
     9361 atcttctgcg actatgccgg attctcaacg cttgattcag tggtgcgtgc caaaaaggtg
     9421 tttggggaaa accatatcac cattatctcg caagaatttc ataaccaacg cgccatctgg
     9481 ctggcaaaac agtacggcat cgacgctatc gggtttaacg cgccagacct taatatgaag
     9541 cacggttttt atactcaact gcgggagaaa ctcgctcgtg tcagcgcggt gattgacgcc
     9601 aaaatcctcc atcgccagcc gaaatatctc gggccatcgg tcatgattgg gccgtttagt
     9661 gagcatggct gtccggcgaa agaataatgc gtatctgcgc acgtcgaaga tgaaaaaggc
     9721 gtgctacatt gacgacagaa tccctttatg gagtatccac gcgttatgat acgtttcgct
     9781 gtgattggta cgaactggat cactcgccag ttcgtcgagg ccgcccatga gagcggtaaa
     9841 tacaagttaa ccgccgtata ttcccgcagc cttgaacagg cccagcactt cgccaatgat
     9901 ttttctgtcg agcatctgtt tacctcgctg gaagcgatgg cggaaagcga tgccattgac
     9961 gcggtgtata ttgccagccc gaatgccctg cacttttccc agacacaact tttccttagc
    10021 cataaaattc atgtgatttg cgagaaacca ctggcgtcga atctggcgga agtggatgcc
    10081 gccattgcct gtgcgcggga aaatcaggtg gtgctgtttg aggcatttaa aaccgccagc
    10141 ctgccgaact ttcatttgct gcgccaggcg ctgccgaaag tcggcaaatt gcgtaaagtc
    10201 tttttcaact attgccagta ttcctcgcgg tatcaacgtt acctggatgg tgagaatccc
    10261 aacaccttta atccggcatt ctctaacggt tcaattatgg atatcggctt ttactgcctg
    10321 gcgtcggcgg tggcattatt cggtgaaccg aaaagcgtgc aggcaaccgc cagtttgttg
    10381 gcaagcggcg tagatgccca cggcgtggtg gtgatggatt acggtgattt cagcgttacg
    10441 ttgcagcact caaaggtcag tgattctgtc ctggaaagcg agattcaggg cgaagcggga
    10501 tcgctggtga ttgaaaaact gtctgaatgc cagaaagtgt gcttcgtgcc gcgtggcagc
    10561 cagatgcagg atctcaccca gccgcagcat attaatacca tgctctacga agcagagctg
    10621 tttgctaccc tggtggatga gcatctggtg gatcatccag ggctggcggt cagtcgcatc
    10681 accgccaaac tgctgactga gatccgccgc cagactgggg tgatttttcc ggcagatagc
    10741 gtaaaactat aattgccaaa gtaaaacagt gtaaaaggta tgtaacagac cattgactgg
    10801 ctgaatggtc tgtcatactt tgttacctgc aaaggggagt aacttcattg ccggtcgatc
    10861 gtcattacga tgtgtgaaaa accacatccg gtcaccgggc aacccgaaag gaatacgcag
    10921 acgtattcct tttttgttgt aagtgagacc ttgccggaag gcgaggtcta tgcataaaaa
    10981 gcagcggctg acgtcttccg acgttggccg tttttttatg tgtaaggaac ttctatgaat
    11041 actgtcggca cgccgttgct atggggcgga ttcgctgttg ttgtcgccat tatgctggct
    11101 atcgacctgt tgttgcaggg gcgtcgtggg gcacatgcca tgaccatgaa acaggctgcg
    11161 gcctggtcgc tggtctgggt gacgctgtcg ttactgttta acgccgcttt ctggtggtat
    11221 ctggtgcaaa ctgaaggtcg cgccgttgcc gatccacagg cactggcctt tctcacgggt
    11281 tatctgattg agaaatcgct ggcggtcgat aacgtctttg tctggctgat gttgttcagc
    11341 tatttctctg ttccggcggc attacaacgc cgcgtgctgg tgtatggcgt gctcggggcg
    11401 attgttctgc gtaccatcat gatcttcact ggcagctggc taatttcgca gttcgactgg
    11461 atcctgtata tcttcggtgc cttcctgctg tttaccggcg tgaagatggc gctcgcccat
    11521 gaagatgaat caggcattgg cgacaagccg ctggtgcgct ggctacgcgg tcatttgcgc
    11581 atgaccgaca ccatcgacaa cgagcatttc tttgtgcgta agaatggatt gctgtacgcc
    11641 acaccgctga tgctggtact gattctggtg gaattgagcg acgtgatttt cgccgtggat
    11701 agcattccgg ctatcttcgc cgtgaccact gacccgttca ttgtgctgac ctcaaacctg
    11761 tttgcgatcc tcggcctgcg tgcgatgtat ttcctgctgg cgggcgtagc agagcgtttc
    11821 tcgatgctca aatatggcct ggcggtgatt ctggtgttta tcggtatcaa gatgctgatt
    11881 gtcgacttct accatattcc aatcgccgtc tcgctgggcg tggtgtttgg cattctggtg
    11941 atgacgttta ttatcaacgc ctgggtgaat tatcggcatg ataagcagcg gggagaatga
    12001 ttcgctgtat attaagccat tcctagagat aactcattcc agacaaaggt gagctattaa
    12061 ttatgtaatc ggcttgtagc tctttacaaa aagaccgcac gggtgttggt ggcgtcaaaa
    12121 tcgccgaagc gttccctgga ggccggggcg acgcgcatgg atgcgcggcg agggcgcatc
    12181 ttacagggac gttcccttgc gcccgacccg gtagccgcaa gggataagct gagggaatc
//
LOCUS       DAZPDU010000128        12225 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZPDU010000128 DAZPDU010000000
VERSION     DAZPDU010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12225
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.129"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..510
                     /gene="csgD"
                     /locus_tag="ACHURS_003176"
     CDS             <1..510
                     /gene="csgD"
                     /locus_tag="ACHURS_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFR1238840.1"
                     /translation="DDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPE
                     DYPYRDIENWPHINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYN
                     STESALLTHREKEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAV
                     SWANDNLRR"
     gene            515..904
                     /gene="csgE"
                     /locus_tag="ACHURS_003177"
     CDS             515..904
                     /gene="csgE"
                     /locus_tag="ACHURS_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFR1238841.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            929..1345
                     /gene="csgF"
                     /locus_tag="ACHURS_003178"
     CDS             929..1345
                     /gene="csgF"
                     /locus_tag="ACHURS_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFR1238842.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1372..2205
                     /gene="csgG"
                     /locus_tag="ACHURS_003179"
     CDS             1372..2205
                     /gene="csgG"
                     /locus_tag="ACHURS_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFR1238843.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQSLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2270..2761)
                     /locus_tag="ACHURS_003180"
     CDS             complement(2270..2761)
                     /locus_tag="ACHURS_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFR1238844.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(2863..3417)
                     /gene="ycdY"
                     /locus_tag="ACHURS_003181"
     CDS             complement(2863..3417)
                     /gene="ycdY"
                     /locus_tag="ACHURS_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFR1238845.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDVTEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3441..4178)
                     /gene="ycdX"
                     /locus_tag="ACHURS_003182"
     CDS             complement(3441..4178)
                     /gene="ycdX"
                     /locus_tag="ACHURS_003182"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFR1238846.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4233..5171)
                     /gene="ghrA"
                     /locus_tag="ACHURS_003183"
     CDS             complement(4233..5171)
                     /gene="ghrA"
                     /locus_tag="ACHURS_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFR1238847.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            5405..5492
                     /locus_tag="ACHURS_003184"
     tRNA            5405..5492
                     /locus_tag="ACHURS_003184"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5439..5441,aa:Ser,seq:gga)
     gene            5537..5673
                     /locus_tag="ACHURS_003185"
     ncRNA           5537..5673
                     /ncRNA_class="other"
                     /locus_tag="ACHURS_003185"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(5764..6450)
                     /locus_tag="ACHURS_003186"
     CDS             complement(5764..6450)
                     /locus_tag="ACHURS_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFR1238848.1"
                     /translation="MSFMAFVTCLTIAFIAGFAVARKLTLRKVASSVIIAKSQPVPSS
                     ATSPAAKASTRSYIPQKTYRDSRWQERDELFQGMVAEGILLERKEWLITRCEKMYFDN
                     LEKWFGHYCHIHCQVSLGRLINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKI
                     VCVIELDDPSHERADRIIRDRWLDTIMTLARVPFVRASVTKLEEIPPVWETRDKIRPL
                     VAATKSEPVH"
     gene            complement(7031..7819)
                     /gene="phoH"
                     /locus_tag="ACHURS_003187"
     CDS             complement(7031..7819)
                     /gene="phoH"
                     /locus_tag="ACHURS_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFR1238849.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGGGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(8440..9711)
                     /gene="efeB"
                     /locus_tag="ACHURS_003188"
     CDS             complement(8440..9711)
                     /gene="efeB"
                     /locus_tag="ACHURS_003188"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFR1238850.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHV
                     SRSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFSLPGVKDANDYLGSALLRV"
     gene            complement(9717..10844)
                     /gene="efeO"
                     /locus_tag="ACHURS_003189"
     CDS             complement(9717..10844)
                     /gene="efeO"
                     /locus_tag="ACHURS_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFR1238851.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(10902..11732)
                     /gene="efeU"
                     /locus_tag="ACHURS_003190"
     CDS             complement(10902..11732)
                     /gene="efeU"
                     /locus_tag="ACHURS_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFR1238852.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
BASE COUNT         3226 a         3168 c         2828 g         3003 t
ORIGIN      
        1 gacgatatat cttcaggctc tattattctt ctggatatga tggaagcgga taaaaagctt
       61 atccattatt ggcaggatac tttgagcagg aaaaacaaca atatcaaaat attgttgcta
      121 aatacgcctg aagattaccc gtaccgcgac attgaaaact ggcctcatat caacggcgtt
      181 ttttatgcca tggaggatca agaacgtgtt gtcaatgggt tgcaaggcgt cctgcgcggc
      241 gaatgctact ttacgcaaaa gcttgccagc tacttgatta cgcattcagg taactatcgt
      301 tataacagca cggaatcagc cctccttact catcgggaaa aagagatcct gaataaactg
      361 cgtatcggcg cgtctaataa cgagatcgct cgttcgttgt tcatcagcga aaatacggtt
      421 aaaacgcatc tttataatct tttcaagaag atagccgtaa aaaaccggac acaagcggtt
      481 tcctgggcaa acgataacct caggcgataa agccatgaaa cgttatttac gctggattgt
      541 ggcggcagaa tttctgttcg ccgcagggaa tcttcacgcc gttgaggtag aagtcccggg
      601 attgctaact gaccatactg tttcatctat tggccatgat ttttaccgag cctttagtga
      661 taaatgggaa agtgactata cgggtaactt aacgattaat gaaaggccca gtgcacgatg
      721 gggaagctgg atcactataa cggtcaatca ggacgttatt ttccagactt ttttatttcc
      781 gttgaaaaga gacttcgaga aaactgtcgt ctttgcactg attcaaactg aagaagcact
      841 aaatcgtcgc cagataaatc aggcgttatt aagtacgggc gatttggcgc atgatgaatt
      901 ctaaataaaa aattgttcgg aggctgcaat gcgtgtcaaa catgcagtag ttctactcat
      961 gcttatttcg ccattaagtt gggctggaac catgactttc cagttccgta atccaaactt
     1021 tggtggtaac ccaaataatg gcgctttttt attaaatagc gctcaggccc aaaactctta
     1081 taaagatccg agctataacg atgactttgg tattgaaaca ccctcagcgt tagataactt
     1141 tactcaggcc atccagtcac aaattttagg tgggctactg tcgaatatta ataccggtaa
     1201 accgggccgc atggtgacca acgattatat tgttgatatt gctaaccgcg atggtcaatt
     1261 gcagttgaac gtgacagatc gtaaaaccgg acaaacctcg accatccagg tttcgggttt
     1321 acaaaataac tcaaccgatt tttaagcccc agcttcataa ggaaaataat catgcagcgc
     1381 ttatttcttt tggttgccgt catgttactg agcggatgct taaccgcccc gcctaaagaa
     1441 gccgccagac cgacattaat gcctcgagct cagagctaca aagatttgac ccatctgcca
     1501 gcgccgacgg gtaagatctt tgtttcggta tacaacattc aggacgaaac cgggcaattt
     1561 aaaccctacc cggcaagtaa cttctccact gctgttccgc aaagcgccac ggcaatgctg
     1621 gtcacggcat tgaaagattc tcgctggttt ataccgctgg agcgtcagag tttacaaaac
     1681 ctgcttaacg agcgcaagat tattcgtgcg gcacaagaaa acggcacagt tgccattaat
     1741 aaccgaatcc cgctgcaatc tttaacggcg gcaaatatca tggttgaagg ttcgattatc
     1801 ggttatgaaa gcaacgttaa atcgggcggt gttggggcaa gatattttgg gatcggtgca
     1861 gacacgcaat accagctcga tcagattgcc gtgaacctgc gtgtcgtcaa tgtgagtacc
     1921 ggtgagatcc tctcctcggt gaacaccagt aagacgatac tttcctatga agttcaggcc
     1981 ggggttttcc gctttattga ctaccagcgc ttgctggaag gggaagtggg ttacacctca
     2041 aacgaacccg ttatgctgtg tctgatgtcg gctatcgaaa caggggtcat tttcctgatt
     2101 aatgatggta tcgaccgtgg tctgtgggat ttgcagaaca aagcagaacg gcagaatgac
     2161 attctggtta aataccgcca tatgtcggtt ccaccagaat cctgacagat aaaaatgccg
     2221 gaattaataa tccggcattt tactgtttaa gcgaaagcgt gagtgcccct cacgctttat
     2281 ttttgatttc ctgctcacgg tgagccgtct ttgcactacg cgccgccagc cacaggccgc
     2341 tgtttttcat tgcgtaacca aataccagcc caagcgccag cgaaggtaac accagtttcc
     2401 agtcaccctg cccggcaaac gtcgcgcatg cgcctataaa cgtccccgga acaaatgaaa
     2461 gcagcagctg tttggcctga atacacatca gaaacgccac aataccggtt atgacataac
     2521 cgagaatttc cagatgtggt gccagtgcac taccgtaaat aatgaccatc gcccacacca
     2581 cgccactaag cagggttgca gcggagatcg ccagcccttt cagcccacct tgcgggcagg
     2641 caaagtaggc cgtacagccc aagaagcccg cccagcttag taagcccagg gaaacagcca
     2701 cccatcccca gataccggag agaatgcctg ttgtgattgc aatagagaga agtatgttca
     2761 tgccgcgcat gatagcagaa aacgcggcca tgaatgagat cgagcacaca ttttatgcaa
     2821 caacaaatta tctgttgcag ttatgagtga cgatgatcac atttactctt cagaatcttc
     2881 ttccagctcg tcccacattg cactaatggc atcacgggtt agcggtgcca tggtgcgcca
     2941 gaaaggcgtg gttgcatggg cctccacttt gccgaggaac gcaccacacc agggtaacag
     3001 atactcactg aacagtgttt ccagtgcttc gctctcatct tccgttgact gatcttccag
     3061 ccaggaagcc gcaagcagca atgtgccgat gtgatcggct ggcgtatccg ccagtggcat
     3121 ccctcgttcg gaaagaaaag cgcgcacttc cgcttccgtt acgtcctcaa cccatgcgct
     3181 acgatatggc ggcactgcac attcatcgcc gataaacaac gcattgtaat cggcagagac
     3241 ttgcgccata tcacagcttt tctgtaaacg cgtcagtaac tcatcctgct ccagtggcca
     3301 gttcgcagcc agtttcccct cacgaatcag ggtaaacagc ggcaccagta aaggatcttg
     3361 cggttggcgg taatagagcg aacccagcac acgacagagg atagaaaact cgttcattaa
     3421 tatatttcca ttactagtaa ttaaaggtct gcaaattccg caatcggtgc cataccgcga
     3481 gattcaagga agttcagtaa gcggcgcgga gaaacattca aaatgcgctc tggcggaaaa
     3541 tctaccgcgt cgaggatttt aagacactct tcaaattccc ccatggtaaa cgcggtgtga
     3601 gaatccgagc ctaatgccac ccaacctcca gcatcacgta ccgctgcggc taccgcacga
     3661 cagttgtctt cactgccctt acgtgagtgt aaaaatgaag aattattgat ttccagcgcc
     3721 acctgatgtt tcgcggctgc ttcagcaacg gctttcacat ctatttcata tttgggattt
     3781 ccgggatggc ttattatatg cacattgccg ctggcgatag tggcgatcat cgcttgtgta
     3841 ttggtcgctt tgtcatgtgg tgcaaaaacc ggctcatgaa aaccggcaat aatcagatcc
     3901 agcgagtcaa acattttacc gctgcaatca atttcaccat caacattttt aatgttagct
     3961 tcgatgccgc gcaggatccc taccccatca accactcgcg gccagatacg catgttaatg
     4021 aagtgccagt gatgcggagc atcttccata tccgggccat gatcggtgat cgcaaaaagt
     4081 ttaatgccct tttgtttggc ctgggcaatg taatcactca atgtgctata tgcatgtgtg
     4141 ctggcaacgg tatgcatatg aaggtcgacg ggatacataa ctctctcctg tattcaattc
     4201 ccgccaagga tagcaggaat cctgatgctt tattagtagc cgcgtgcgcg gtcgacttgc
     4261 ccgcagaccc tctccccttt ttcgagctgg gcaatggtgc gagaaatgta ctccacagct
     4321 tcagcgggac gggtaatcgc ggcgacatgt ggtgttatcg tcacgcgtgg atgttgccag
     4381 agcggacttt caggcggtaa gggttcacga ttaaaaacat ccaacattgc gcctttaact
     4441 ttgccgctat ccagcgccgc gagcaggtca tcttccacaa catgaacacc acgcgccagg
     4501 ttgaggagat acgcgccatc cggtaatttt tcgagtaatt gttgattaat aatgccgacg
     4561 gtttcagggg tattcggtaa caaattaatc aatacccgac attggctcag aaatgcagac
     4621 agttcttccc gtccggcaaa gctttgcacg ccaggccacg atttacgggt tcgactccag
     4681 caacgcagcg gaaagcgcca ggtttgcaga ctctgagcaa ctttactgcc cagtacgcct
     4741 gcgcccaaaa tgccgatggt aaaatcttcc cgatgatatt caggcagcgg ttgccaatgc
     4801 gaactatttt gctggatgcg ataatcgtca aaacgtcgaa accaatgcag cacctgactg
     4861 acagcatatt cctgcatttg ctcgcccata ccggtatctt ccaggcgaaa aagtggaaca
     4921 gaagggttca gcatttcagg gtgtgcctgt agcttgctca aaatagaatc aacaccggcc
     4981 ccgagtgcga acaccgcttt aagatcgcgc cctgccagca tttcaacagg aggatgccag
     5041 actaaagcat aatcagcaga gtcattatct ccgcttttcc atgctctgac tcttgcctga
     5101 ggaatagctt tgcgcagtgc ctcaatccac cattgggtat cgaacgttgg gtgataaaag
     5161 atgatatcca tactgactcc cgaaaagcgt tgtgcgaaat ttattcgcac ttatcgttat
     5221 gatctacaaa ggccaccagc ataacaaatc cgtggtcggt ggcaaaaaaa gcagatttcg
     5281 cttattaaaa ccacacattg attgaagttt gaataaacgc gcgatttttt caaaaagttt
     5341 gttgacctca ggtcatgatt tccctaaatt agcgcccgtt ccagcaagac aggaacgaca
     5401 atttggtgag gtgtccgagt ggctgaagga gcacgcctgg aaagtgtgta tacggcaacg
     5461 tatcgggggt tcgaatcccc ccctcaccgc catatttaaa gaagagctcg tacgaaagta
     5521 cgggcttttt tttcgtatat tgcacacacc ggggggatga gaagccccga ccggggttcg
     5581 acaactggcg acagccagtt ggacagaccg tgaacgcagt gagcgggctg cccgcagggc
     5641 gagcgaagcg agtcaatccc cccctcaccg ccatctttaa agaagagctc gtacgaaggt
     5701 acgggctttt tttcgtatat tgcacacacc ggggggatga gaagccccga ccgggatact
     5761 tagttaatgc accggttcac tctttgtcgc cgcgactaat gggcgaattt tgtcacgagt
     5821 ctcccagacc ggaggaattt cctccaattt agtgacagaa gctcgtacaa aaggcacccg
     5881 tgcaagagtc ataattgtat ccaaccaacg gtcacgaata atgcgatccg ccctttcatg
     5941 actcgggtca tccagctcaa taacacagac tattttattg ttcttctttg aaaccagaac
     6001 ataatccatt gccatgccat tataaattgc aaagaaacgc tttctttctt cttcgctaaa
     6061 ctcaggttgt tctggaaagt tgatcaagcg ccccagagag acctggcaat ggatatgaca
     6121 ataatgtcca aaccattttt ccagattatc gaaatacatt ttttcacaac gcgtgattaa
     6181 ccactctttt ctttccaata aaataccttc ggcaaccatt ccctgaaata actcatctcg
     6241 ctcttgccaa cgagagtcac gataggtttt ctgcggtata tagctccttg tcgacgcctt
     6301 agcggcaggg gaagttgcag atgaagggac gggttgcgat ttagcgatga ttaccgatga
     6361 ggcaactttt cggagtgtca attttctggc aacagcaaaa ccggcgataa atgcaattgt
     6421 caaacaagtt acaaaagcca taaagctcat aattaatttt cacctatcca tgttgattat
     6481 atttcattac caatgttact cgctgcagcg tgttacatgc caacaatgca acgatcaata
     6541 tatagttagt atcatgtttt gattattaca accatcccta tttagccatt gcggcttgcc
     6601 ttccacaaaa aaataacaac acacaatatt agcaaagaat taattaagat taaccttaca
     6661 tttataatat tatccctaaa gaataaatca gaggaatata ttcggtgtta aacagtcagt
     6721 caaagtatag cccattgttg attaaacctc ataagcaaaa ttggcaccag atctcctgta
     6781 caggttgaat ggccgaaaaa agataggcag atgctcattt ttttggcttc tggaagaaaa
     6841 actctccgcg ctggcagtat tacactggtc tgatgctatg acgaaaaatg cctggtaaga
     6901 gtatctaaac cagactccag ttcagcttat caacacgctc aaagtcgccc ccctgaacgc
     6961 taacctgaag tgaaaatctg ttgaaataaa acaaagcccg gttcgcccgg gctctgcacc
     7021 gataacacac ttaactgtag gcatgcagcg tacgttggca aagtgccgaa cgtacgcagt
     7081 cctctttacc gaaccggacg atcccaacca tttcatcttc ttcgaaacgt tccagcgcgt
     7141 cacttaatcc ggagcacacg ccgcgaggca aatcgcattg cgtgatatca ccgttgacga
     7201 taaccgtcac gttctccccg aggcgggtta aaaacatttt catttgcgcg gcagtcacat
     7261 tctgcgcctc gtcaagaatg acgactgcat tttcaaaggt acgtccacgc atataggcga
     7321 acggcgcaat ttccaccttc cctatttccg gtcgcaggca gtactgcata aaggaagccc
     7381 ctaagcgccg gaccagcacg tcgtagaccg ggcgaaaata gggagcaaac ttttctgcga
     7441 tatctccagg taagaagcca agatcttcat cggcttgcag aactggacgg gtgacgataa
     7501 tcctgtcgac atccttatgt atcagggcct ctgccgcttt tgctgcgctg atccaggttt
     7561 ttccgccccc ggcttcgccc gtggcgaata tcagctgctt actctcaata gccttcagat
     7621 agtgcaattg cgcttcattt cgcgcgagga tgggcgaagt atcgcgactg tcgcgggcca
     7681 taccaatggc ttctacgccg cccatctgca caagcgaggt gaccgattct tcttcacgct
     7741 gcttatggct gcgcgaatcc cgtctcagca cacgttttgc ctcgcgacga gctttgatca
     7801 ctgctttttg tcttcccata gagagcacct tgagttgttt gtattcatca cacgcgccgt
     7861 tggcagcgcg attatgcgca cgaacatcag agggttggct tccttgtaag ccatagtttg
     7921 cttttggata aaatgccgaa aaacggctac gcgcaccgtt tacggcgtcg gtaacacatg
     7981 aaaagaaagg atgaggttga aaatgcaaag tgacgagatg actaccggag gagaaaactc
     8041 cgcgagtggt ggcgcgttga ttatctaaaa catgtccagt acaggacgtt accatccgcg
     8101 atctccatag tgactgacta tcactgccgg gaacttccgc tgctacttaa taagtacaac
     8161 agatctcgca tttattgcaa caatatattt acttatattt aactataaaa cactatttca
     8221 gtgacattag tttctactgg aaagatgaca gagtgatgac agtgatgaaa aaagctgtgt
     8281 gctttcagca ggatttgcat caggcatcag tgcacgatcg ccggatgcgg cgtgaacgcc
     8341 ttatccagcc taccaaaccc gcactgattg taggcatgat aagacgcgca agcgtcgcat
     8401 caggcatctg cgcacaaatg ccttatccgc ctaaaaacat taaacccgca ataacgcgct
     8461 tccgagataa tcgttcgcgt ccttcactcc cggcagcgaa aaaaaataac cgccgccgat
     8521 aggtttaacg tattcctcca gcgcttcgcc attgagcctt ttttgtactg tcaggaagcc
     8581 tttttccaga tcgtgttggt agcagacaaa cagcaacccc atatccagtt gtccggagtt
     8641 ggtgaccccc aacgaataac tgtagccgcg acgcagcatc aggctggact cactctccgc
     8701 cgtgcgggga ttcgccagcc ggatatggct gtccagcgca atcaccttcc cttccgggtc
     8761 gctggcgtaa tcaggcacat catgctcatg ctgcattccc agcggcgcac cggtttgctt
     8821 atcacggcca aaaatcgtct gctgttcttt cagcggcgtt ctgtcccaaa attccactcg
     8881 aaactgaatc aagcgtactg cctgatagct gccaccgatt gtccacgcag gctcctgctg
     8941 atctgccgtt acccacacca ctttttgcat caacttatca ttctggctat cgggattggc
     9001 agtgccgtct ttgaaaccca gcaaattaat cggcgtctct ttgcctttac tacgcgacac
     9061 gtgatcggaa ataaaccctt cccgcttcca gcgcacactg agcaaatccg gcgtgtgttt
     9121 gatgatatcg cgcagcgcat ggataaccgt gtcctgggtg ttggcgcaaa tctgtagcaa
     9181 cacatcacca tgacataacg ccgcatccag cgagtcgttg gggaaacgcg tcatcttctg
     9241 cagctttttt ggcatctgtg gcgcaaggcc aaagcgctca tcaaacaatg agtgacccac
     9301 cgataacgtg atggtgagat tatcgggcgc aatgtagccg ccaagaatgc cggaatcgag
     9361 tggtggcagg cgcggatttg gcgtttctgg tgctgctccg ccctgagtca gaaaagcaaa
     9421 acgctgagtc aacaagcgaa acaaccgctc aagatcggct ttatcgctgg caagcacatc
     9481 aaacgccacc agcatcattg cggcctgttg tggcgtcagg atccctgcct gatgctcacc
     9541 ataaaacggc tgtttctcat tgcgtgcatc cggtgaaagc gtgcccggtg cactttgcgt
     9601 tttttgtgca tgagcgaccg gacaacttcc cgccagcgcc agcgcaccaa tccctttcag
     9661 taaacgtcgg cgtgacggtt cattcacgcc gttttcatct ttatactgca taacgcttaa
     9721 tccagtccca gcacaccgcg aagttgcgcc agatcttccg ccagcgcagt aatcggtcct
     9781 ttcagtgcat tccggtcggc atcggtcaat ttgtcgtagg tttcaaaacc gtctttagta
     9841 cggtatttcg ccagaatggt atcgaccttt ttaaagttgg catcgacttt tgccagcagt
     9901 tccgggttgg ctttttgcag ttgtggacgc agcaaatcga caattttctg cgagccttca
     9961 acgttagcct ggaaatccca cagatcggtg tggctgtagc gatcttcttc accgctaatt
    10021 ttgctggctg ccacttcctc aatcagtccg gctgcgccgc cgaccacttt tgaaggtggg
    10081 aaagccagtt cactgatgcg tttttgcaaa tcgaccacat ctgtataaag ctgctcagcg
    10141 tactgatcca tccctttggt ggtgttgtcg ccaaacaatg ctttttccag acggtggaaa
    10201 ccagtgaatt ttgggtcggc ggctttttgc tcgtaatcat cttcacgggc gtcaatgctg
    10261 ccatccagat cggagaacag ttcagcaatc ggttcaatgc gctcatagtg ctggcgcgtc
    10321 ggcgcataca gtgctttcgc tttttcgata tcgcctgctt taatcgcgtc ggtaaaggct
    10381 ttggtgtcgg tcaccagctg cgtggtttcc gccatgacat acgctttata tgcagtaatt
    10441 gcaccaccaa gacttaacag cgcatcactt tgcgccgcat ccgccgttgc ctcacctttg
    10501 acgatcaact tccctttcgg gttagtcagc agaccgcagg tcatatcgta ttcgccaggc
    10561 tgtaaattcg ccgtcatttt ctggctaaaa ccaggggcga tattttcccg ctcttccacc
    10621 accatcacgc ctttgaggat ctcccactcc agcgccttct ggctatggtt ctgaataatg
    10681 aactgtgttt tcccggcgtt aaccgtaatg gtcatcggtt cgcactgctt atccgtcacg
    10741 gtcactttga cctgcggcac atcagcggcg ttagccataa aagcagaaga aaacagcgca
    10801 gccacgctca actgcaatgc gttacggcgg aagttaatgg tcatgacgag actatccctt
    10861 taaagtatgt tgtaactaag caagagtttg cgtcgtattt gttacgcgga gcgagacgct
    10921 gtcgcccctg cgcgtggtgg cagagcaaat gccaccagcg ccgggatgag ataaataaac
    10981 cagacggcga cttcgctgac gctcggtgct tcctgatagc caaaaatccc ttccatcagc
    11041 gtgccaaaca gcgagtgagt tgagagcact gcgctcatat cgaaggcgat ttcctgaaag
    11101 tggttccaca atccggcttc atgaaatgcg cgaatggcac cagcagccag ccctgcggcg
    11161 acgaagagaa taaacaggct ggtccattta aaaaatgcac caagattgag gcgaataccg
    11221 ccccagtaga gcaggaagcc tagcaccacg gcagtagcaa gaccgagcat tgcacccagc
    11281 ggcggccaga tcccgacatc ttgttgaaat gccgccagca ggaaaaagac cgactccagc
    11341 ccttcccttg caacggcaaa aaagaccatc atcaccagcg cccagccatg atgatttcca
    11401 cgctgcaatg cgctatcgac tgcctgctcc agttgtactt tgacgttgcg cgacactttg
    11461 cgcatccaga aaaccatcca ggtaaggatc accacggcga tcaccgccac gataccttca
    11521 aacagttcct gttctttttg cggaaattcg ccggtggttt cgttaatgaa gatacccaag
    11581 cccaggcaca acgcagcggc aagcaacacg ccaatccaca tcacgccaat ccatcggcct
    11641 cgctgggtac gcttaagata gctggcaatc aaactgacaa tcagcgcggc ttcaagtcct
    11701 tcgcgcaaca taatgagaaa cggaacaaac atgccaccca cccttaaacg tttttctgag
    11761 tcaatccatg caaagaaaag taaatcgcag tgatagcgat tatcattacg gcaaagaaaa
    11821 atacaagcgg aatgtttata aaatgatgtt ggaatgtaaa caaagagagg taataccagg
    11881 ggatataaac gattattcca ggtacaaaga tgggattgtt tacaacgagc catgtgttct
    11941 tgccagatgc cgcgtaaacg ccttatccag ccgacaaaac tgtccagact caatgcatca
    12001 cagcttccat gtaaaactga taagcgggtg tatcaagcaa tttcgtccat ccgcgattat
    12061 caaatctaac tttttgcccg gaacataata acagcgcggg tgttggtggc gtcaaaatcg
    12121 ctgaaacgtt ccccgaaggc cggggcagcc cacatggatg tgggctgagg gcgcgtctta
    12181 caggacgtta cctcgcgccc gacccggtag ccgaagggga taagc
//
LOCUS       DAZPDU010000129        12091 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZPDU010000129 DAZPDU010000000
VERSION     DAZPDU010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12091
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.130"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            28..744
                     /locus_tag="ACHURS_003191"
     CDS             28..744
                     /locus_tag="ACHURS_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFR1238853.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNAAQKLVNAGNQRLKILTIAYCKNNSKENCKIQTVNKNIFLGQERNLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
     gene            774..1274
                     /locus_tag="ACHURS_003192"
     CDS             774..1274
                     /locus_tag="ACHURS_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CS1 type fimbrial major subunit"
                     /protein_id="HFR1238854.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            1366..4050
                     /locus_tag="ACHURS_003193"
     CDS             1366..4050
                     /locus_tag="ACHURS_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HFR1238855.1"
                     /translation="MDKKLLALLILASLSPAEATLTEIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSCYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            4047..5135
                     /locus_tag="ACHURS_003194"
     CDS             4047..5135
                     /locus_tag="ACHURS_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
                     /protein_id="HFR1238856.1"
                     /translation="MRNRLIAAILGLFGTLIGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGDFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(5218..6515)
                     /gene="exuT"
                     /locus_tag="ACHURS_003195"
                     /pseudo
     CDS             complement(5218..6515)
                     /gene="exuT"
                     /locus_tag="ACHURS_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
     gene            6998..8410
                     /gene="uxaC"
                     /locus_tag="ACHURS_003196"
     CDS             6998..8410
                     /gene="uxaC"
                     /locus_tag="ACHURS_003196"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFR1238857.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            8425..9912
                     /gene="uxaA"
                     /locus_tag="ACHURS_003197"
     CDS             8425..9912
                     /gene="uxaA"
                     /locus_tag="ACHURS_003197"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFR1238858.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            9995..10546
                     /locus_tag="ACHURS_003198"
     CDS             9995..10546
                     /locus_tag="ACHURS_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFR1238859.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(10551..11795)
                     /gene="sstT"
                     /locus_tag="ACHURS_003199"
     CDS             complement(10551..11795)
                     /gene="sstT"
                     /locus_tag="ACHURS_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFR1238860.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
BASE COUNT         3263 a         2871 c         3080 g         2877 t
ORIGIN      
        1 gaaacaatat tgtaagatca aatttatatg gtggcgaaaa ttaatctctt atcacggctt
       61 acgccgcttt tattcgtgtt cgctcctttt ttggcacagt caaatatgac tgtttatcct
      121 atggctgttt ccattaatag tcagggtgaa ggcaacgttc gcgtaatatc taaaagtaat
      181 gaagttcagt acatcaaggc gacagtattc cgtatcgata atccatcgac gcctcaggaa
      241 aatgaagttg agattaaatc cggtgatgca aatcatctgg ttgttatgcc acctaaattc
      301 gctttaccag ccggtagtag caaaactgtg cgctttgttg caatggaacc agagcaaaaa
      361 gaaaaaaatt atcgcgttaa atttgaagcg gttcccagta ttgatgacgt tgccacagat
      421 aaaaaagatc tctctatgca gttaacagtt aacttaattt gggggattgt tgttagtgtt
      481 ccacctcagc aacctattgc taagttagaa gtaaatgctg cccaaaaatt agttaatgca
      541 gggaatcaac gcttaaagat tttaacgatc gcttattgta aaaataatag caaagaaaat
      601 tgtaagatac agaccgtaaa taaaaacatc ttccttggtc aggaaagaaa tcttgaaagc
      661 atatctggct acgacaaaat cgttgtcaaa tataataact ggatcaccaa agataatggc
      721 gagtttgaac tggcggtcca ttaattatca ttgtaataaa cgaaggaata gtaatgaaaa
      781 aggtatttgc aaaatctctt ctggtcgcag cgatgttttc tgttgcaggc tccgcattgg
      841 cagtacaaaa ggatattact gtaacggcca acgttgatgc cgctctggat atgacacaga
      901 ccgataacac cgcgttgccg aaagcagtag aaatgcaata tctgccgggg cagggtctgc
      961 aatcttacca gttgatgacc aaaatctggt ctaacgacgt taccaaagat gtaaaaatgc
     1021 agttagtttc cccggcgcaa ctggttcaaa gcctggatgc tagcaagatt gttccgctga
     1081 cagtaacctg gggcggtgaa gaaattaaag ctgatgcggc aaccactttt accgcaacca
     1141 aaatctttgc ttccgatgcg ttaactaacg gttctctggc taaaaacctg atgtttgctc
     1201 aaaccaccaa aggtgttctg gagacaggca tctaccgtgg tgtagtaagc atttatctgt
     1261 ctcaggatat ctaatccaga taacagaatt aacacgtaac aaagggaggg gtagccctcc
     1321 cttattttaa ctgttttagt atttgttatg gatttcagga ttgccatgga taaaaaatta
     1381 ctggctcttt tgatcctggc gagtctcagc ccggcagagg cgacattaac cgaaattccc
     1441 gcagggtttg aggttattgc tcagggacag caggagtata tcgaggttta tttttcaggg
     1501 aaaagtctcg gtaaatatta tgcaatggtt aatcttgata ccgtaacatt tcttgatcca
     1561 gcaagtctat ataacaagct ggaactggat gtagacgatc agaaaatcgc gcatatagtg
     1621 aaagaaaaat tatcgcagcc gctagctcgc cacggtgaat tggcttgcgg ttatgtacgt
     1681 actgactcag ggtgtggttt tctgaatacc gatacgctgg aaataatcta taatgatgaa
     1741 gaaagttcgg caacgttgtt tattaatccg caatggaatt cagctttcga tgcgaagtca
     1801 ttatatttaa atccagacaa aaatactgtt aatgctttta tacatcagca agacatcaat
     1861 gttctggcac aggatgatta ccaatcgttg tctattcagg gaaacggtgc gctgggaata
     1921 acagaaaata gttatattgg tgcacactgg aatttcaacg gttatgatgc agatgatgtc
     1981 agtgacagta atgctgacgt cagcgatctc tattatcgtt atgatttttt acgtcgttat
     2041 tatgtgcagg cgggtcgcat ggacaaccgc acactattta atgcacaagg cgggaacttt
     2101 acctttaact ttctgccact cggtgcaatt gatgggatgc gtatcgggtc gacactgagc
     2161 tatttaaacc aggcgcaaag ccagcaagga accccagtaa tggttctgct ttcgcgcaat
     2221 tctcgtgttg acgcttatcg taatgagcaa cttttgggat cgttttatct caatagtggt
     2281 tcgcaattta ttgataccag ttcctttccg ccaggcagct acagcgtggc attaaaagtc
     2341 tacgaaaata accaactcac ccgcaccgag cttgtgccgt ttaccaaaac cggcggtctg
     2401 actgacggaa atgcgcaatg gttcttacag gcaggtaaaa ctacatcaca ggtttctgat
     2461 gatgaaagtt cagcttatca gctaggagta cgcctgccat tacatccgca atatgagctc
     2521 tacgcagggc tggcgaatgc cgatgatgtg agtgctttcg agttaggtaa taactggacg
     2581 gcagatttag gcggggtggg gaatcttgca atcagcgcca gcgtgttccg taacgatgac
     2641 ggcggcaaag gtgatatgca acaggccaac tggagtaatc cgggatggcc gacgttgggc
     2701 ttttatcgga ccaactctga cggcgatgct tgtacaaccg acagcagaga gagctataac
     2761 gccttaagct gttatgaaag tatttccgcg acggtttcac agaattttgt cggctggaat
     2821 atgatgctgg gttatacccg cacacaaaat aacactgatg atagtttgcg ttgggataaa
     2881 cagcagagct ttgaaaataa ctatcttcgc cagacaacag cgcaaagtat ctctgaaact
     2941 gtacaactta gcgcttcccg cgcttttgtg atgcgtgact ggattttgag tacgtcagtc
     3001 ggtgttttcc atcgtaatga caacggtggc gataacgacg acaacggctt gtacttatcg
     3061 ttttcgttat ctgacacgcc aacgatggat agcaataaca acagccattc aacaaatgtg
     3121 tctacggatt atcgttatag cgaacaggat ggcgatcaaa cgtcatggca gttatcgcat
     3181 accttttata acgattcatt cagccataaa gaacttggcg taaccgtcgg aggcctgaac
     3241 accgatacca taaacagcgc ggttaacggg cgttgggatg ggcaatacgg aaatgtctac
     3301 gctaccgtat ctgatagtta tgaccgtaag aatcatgatc atctttcggc ctttacgggc
     3361 acttatagct ctacgctggc tgtcagttgc tatggcgtta atttgggagc cagtggtaca
     3421 gacgatttgc tgggtgcggt actggtggat gtgaaaggct tctctgaaca ggatgaagag
     3481 agtcaggatc tgcaactcga agcgcgggtg gcaggcagcc gaactttgca gcttggtcaa
     3541 agtgacagtg tgttgttccc ttatcctgga tttcagtctg gttttgttga ggttaacgac
     3601 agtagccagg gcaatcagca ggggacaaca aacatcatta acggtgcagg gaatcgtgaa
     3661 ttaatgttgt tgcctggcaa gctgcgctat cgcgaagtgt ctgccagctt taactacaac
     3721 tatatcggtc gtttattact gccggcagcg gtgaaaaaat tcccgattgt ggggctgaac
     3781 agcgccatgt tactggtagc tgaagatgga ggatttacac ttgaaattaa cggcagcgaa
     3841 aaagagctct atctgctttc cgggcagcaa ttccttaagt gtccactgag tgttgtaaag
     3901 aaacgcgcca gcattcgtta cagcggagat gttacttgta gtgtggtgac ttattcacaa
     3961 ttaccggagt cgattcaggt tcaggcacag ttgaaacagc ctaaattacg tggaaacgtt
     4021 cagacggcgc aaagggaggt tgcaccatga gaaatcgatt gattgcggcg atattgggct
     4081 tgtttggcac gctcattggc gttcaggcag ctcctgacgt gaccagtgaa attacgtatg
     4141 atttggcatc tggtagagcg gattattact tctggaagga tgaagcgtcg gcaggaaata
     4201 atggatatat gtggtatgaa tgttcgtatc ctgacctcca acaaacctgt acagctaatg
     4261 gaaatatatc gacagtacaa atctatttaa ctgaacaacg cagtgggatg cgttggccgg
     4321 taaaactcaa aggatttaaa acagccattg taagtagcga tgaagcgccg ccaggatgca
     4381 aggggggcaa agggcttcag acgaatctta aggattctaa tagatcttca tgtacagaag
     4441 atggtcaaca ttattatata tacgatacaa agtttcttac gctctacctt gagcagacag
     4501 agatgaagaa tttgccgatt ggtggcgtct ggaaggggaa agttaaatta cattcgaaca
     4561 gcccggccca ggactatttc gcaaatatta ccctgaatac gctcgacccc aaccatattg
     4621 acgtgttctt cccggagttc gcccacgcca cgccaagggt gcagttagac ttgcatccaa
     4681 caggaagcgt taacggcagc aactacgcgc aagatctgac catgttggac atgtgcctgt
     4741 acgatggttt taacggtaat gccatcagtt atgaaatcat gctcaaagat gaagggcgac
     4801 cagctgcagg gcgcagagac ggtgacttct ctatctatcg tcaaggaggg accaccaccg
     4861 acgagggaga acgcattgat taccgggtca aaatgtacaa cccggaaacc ggtgggcaaa
     4921 ttgatgtgcg caataatgaa aatatggtct ggaacagcat taacctgaaa cgtgtgcgtc
     4981 cggtggtact gcccggtatt cgctatgccg tgatgtgtgt gccgacaccc ttgactctgg
     5041 cagtcgataa attcagcgtg atggataaac aggccgggta ctacatgggc aaattgtcag
     5101 taatctttac gccttccttg ccaaccatca attgaccccg cagaccagaa aaaactaaac
     5161 aacgaagccg cctgataagg cggcttcgtc attacaactt acgttaaacg ttaaacgtta
     5221 atgttgcggt gcgggatcgt tatgtgtttc ctgcgccacc tcaatcgccg gtttgttctg
     5281 caatacggtc cagataacca gcgcacccag caggtcaaat actgccagaa ctgcgaacag
     5341 cgggctgaag ccgatggtgt cagccagtgc accgacaacc agcgcaaaca gcgtacttgc
     5401 cagccatgcg gacatcccgg ttaaaccgtt cgccgtcgcc acttcgttac gaccgaacac
     5461 gtcagaagag agcgtaatca gcgcgccaga cagtgcctgg tgggcaaaac caccgataca
     5521 cagcagcata attgcgacat acgggttggt gaacagaccg atcatacccg ggccaatcat
     5581 cagcacagca cccagcgtta cgaccatctt acgggaaacg atcaggttca caccaaacca
     5641 acgctggaac agcggcggca ggtaaccacc gaggatacaa ccgaggtcag caaacagcat
     5701 cggcatccag gcgaacatcg cgatctcttt caggttaaag ccgtaaactt taaacatgaa
     5761 cagcgggatc cacgcgttaa aagtacccca ggccggttct gccaggaaac gcggcagtgc
     5821 gataccccag aactgacggt tacgcaggat ctgaccaacg gacattttct tcgccgtgct
     5881 cacctggtgc tgggcttcct gaccattaat aatatagtcg cgttcttcat cggtcagatg
     5941 cttctggtcg cgcggatgtt tatagaaaat cagccatgcc atcgcccaga taaagctcaa
     6001 tgcaccggag atgataaatg ccatctgcca gctgtgcatc acgattgcca taccaccagc
     6061 ggcggcgcaa tcatcgcacc aatcgaagaa cctacgttaa agtagcctac tgcgatggaa
     6121 cgctctttcg ccgggaacca ttcggagctg gctttcagac ccgccgggat catcgctgct
     6181 tccgcggcac cgaccgcacc acgagcaaca gccaggccac cccagctacc tgccagcgcg
     6241 gttacaccac agaacacggc ccacagcaca gcaaacattg cataaccgat tttcgtaccc
     6301 agcacatcca gtacataacc tgctaccggt tgcatgaccg tataagcagc agaataggct
     6361 gcgatgatat aggaatactg ttgggtggag atgtttaact cttccatcag agttggcgca
     6421 gctgccgcca cagtgttacg cgtcaggtaa ccaagcacgg tgccgagcgt caccagtgcg
     6481 atcatatacc aacgtaaccc tttaatttta cgcatgtaaa acctcatcgt tttgttatat
     6541 ccgcaggaag cgcggccatc atcccgccag aggctgaaga tgttagttac gaccgggtaa
     6601 ccgaaaaaaa accggcacgc cccgaacgtt gccatgaaag tttcgtgcta attcggcttc
     6661 cgtaccggtt aacccgattc atctaacaaa tgaatgaatc ggcaatttgc attccgtcga
     6721 cttatgattt gcgacggcag aaagataact tgtcatacaa ctttaaaagg tgagagccat
     6781 cacaaatgtg cgaatatttg tagggacatt acctgacgac agcgaggcca gtactggcgc
     6841 ggcctgcagc gagatttatc actttgagag taattttttt aactacgttt attgatctaa
     6901 ctcacgaaaa tatcttcgga ctctggaaat tggtgtgata actttgtcag catcgcacca
     6961 taagcaagct agctcactcg ttgagaggaa gacgaaaatg actccgttta tgactgaaga
     7021 tttcctgtta gataccgaat ttgcccgccg tctgtatcac gactacgcaa aagaccagcc
     7081 gattttcgat taccattgcc atttgccgcc gcagcagatt gcggaagact atcgttttaa
     7141 aaacctgtat gacatctggc tgaaaggcga tcactacaaa tggcgcgcta tgcgtaccaa
     7201 cggtgtggcc gagcgtctgt gtaccggtga tgcatctgac cgtgaaaaat ttgacgcctg
     7261 ggcggcgact gttccgcaca ctatcggcaa cccgttatac cactggacgc accttgaact
     7321 gcgtcgtccg tttggtatca ctggcaaatt gctttctccg tcaactgccg atgaaatctg
     7381 gaacgaatgt aacgaactgc tggcgcagga taacttctcc gcgcgcggca tcatgcagca
     7441 gatgaacgtg aaaatggtcg gcaccaccga tgacccgatc gattctctgg agcatcacgc
     7501 agagatcgcc aaagacggct ctttcaccat caaagtgctg ccgagctggc gtccggacaa
     7561 agccttcaac atcgaacagg cgacctttaa cgactacatg gcgaagctgg gcgaagtttc
     7621 cgataccgac attcgccgct ttgctgacct gcaaactgcc ttgaccaaac gtctggatca
     7681 cttcgccgct cacggctgta aagtgtctga ccatgcgctg gacgtggtga tgtttgctga
     7741 agcgaacgaa gcggaactgg acagcattct ggcgcgccgt ctggctggcg aaaccctgag
     7801 cgagcacgaa gtggcacagt tcaaaactgc ggtactggtg ttccttggtg ccgaatatgc
     7861 acgtcgcggc tgggtacagc agtaccacat tggcgcgctg cgtaataaca acctgcgtca
     7921 gttcaaactg ctggggccgg atgtaggctt tgactccatc aacgaccgtc cgatggcgga
     7981 agagttgtct aagctgctga gcaagcagaa cgaagaaaac ctgctgccga aaaccattct
     8041 ctactgcctg aacccgcgcg ataacgaagt gctgggcacc atgatcggta acttccaggg
     8101 cgaaggtatg ccgggcaaaa tgcagttcgg ttccggctgg tggtttaacg atcagaaaga
     8161 cggtatggaa cgtcagatga cccaactggc gcagctcggt ctgctgagtc gctttgtcgg
     8221 tatgctgact gacagccgta gcttcctgtc atacacccgt cacgaatact tccgccgcat
     8281 tctgtgccag atgatcggtc gctgggtgga agcgggcgaa gcaccggcgg acatcaacct
     8341 gctgggcgag atggtgaaaa atatttgctt taacaatgcg cgtgactact tcgccattga
     8401 actgaactaa ggtctggggt tgatatgcaa tacatcaaga tccatgcgct ggataacgtc
     8461 gcggtcgctt tagcagattt ggctgaaggc acagaagtca gtgtcgataa ccagactgtt
     8521 acgctgcgcc aggatgttgc tcgtggacat aaatttgcgt taacggatat cgcaaaaggg
     8581 gccaacgtca tcaaatatgg cctgccgatt ggttatgcat tggcggatat tgcggcgggg
     8641 gaacacgtcc acgcccacaa tacgcgcacg aatctgagcg atctggatca gtatcgctat
     8701 caacctgatt ttcaggatct gcctgcgcaa gcggcagatc gtgaagtgca gatctatcgt
     8761 cgcgctaacg gcgatgtcgg ggtgcgtaac gagctgtgga tcctgccaac cgtgggctgt
     8821 gtcaacggca tcgcgcggca aatccagaac cgtttcctga aagagaccaa caacgccgaa
     8881 ggtaccgacg gcgtgttcct cttcagccac acctacggct gctcacaact gggcgacgat
     8941 cacatcaaca cccgcaccat gctgcaaaac atggtgcgcc acccgaacgc gggcgcagtg
     9001 ctggtgattg gtctgggctg tgaaaacaac caggttgccg cattccgtga aacgttgggc
     9061 gatatcgatc ctgaacgcgt tcatttcatg atctgccaac agcaggatga tgagatcgaa
     9121 gccgggatcg agcatttgca tcagctgtat aacgtgatgc gcaacgataa acgcgagcca
     9181 ggcaaactca gcgaactgaa gtttggtctg gagtgcggtg gttctgacgg tctttctggt
     9241 attactgcta acccgatgct ggggcgtttc tctgactacg tgattgctaa cggcggtact
     9301 accgtactga ccgaagtgcc ggagatgttt ggcgcagagc agttgctgat ggaccattgc
     9361 cgcgacgaag caacgtttga aaaactggtc accatggtca acgactttaa acagtacttt
     9421 attgcccatg atcagccgat ctacgaaaac ccatcaccgg ggaacaaagc gggcggtatc
     9481 accacgctgg aagacaaatc acttggctgt acccagaaag cgggttccag tgtcgtggtt
     9541 gacgtgctgc gttacggcga gcgtctgaaa acgcctgggc tgaacttgtt aagtgcgccg
     9601 ggtaacgatg ccgtagcgac cagcgccctg gcgggtgcgg gctgccatat ggtgctgttc
     9661 agtactggtc gcggcacgcc atacggtgga tttgtgccga cggtgaaaat cgccaccaac
     9721 agtgaactgg cggcgaagaa aaaacactgg atcgactttg acgcgggtca gctgatccac
     9781 ggtaaagcga tgccgcagtt gctggaagaa tttatcgaca ccatcgttga gtttgccaac
     9841 ggtaagcaaa cttgcaacga gcgtaacgac ttccgcgaac tggcgatctt caaaagcggc
     9901 gtaacgctat aaagtcgaaa tgagttgtta ataaaaaacg gcgtttcata atgtgaagcg
     9961 ccgtttgctt ttccatacct gataaggatt tatcatgacc gcgtattggc tggcccaggg
    10021 cgtgggtgtc atcgcctttc tgattggtat cacaacattt ttcaatcgtg acgaacgtcg
    10081 cttcaaaaag cagctttcgg tctatagcgc cgttattggc gtacattttt ttcttctggg
    10141 cacctatccc gctggtgcca gtgccatcct taatgccatt cgtacattga ttaccttacg
    10201 cacgcgcagc ttatgggtaa tggcgatttt tattgtgctg actggcggaa ttggcctcgc
    10261 gaagttccat catcctgtcg aactattgcc ggttatcggt acgattgtca gtacctgggc
    10321 gctgttccgc tgtaaagggc tcaccatgcg ctgcgtgatg tggttttcaa cgtgttgctg
    10381 ggtgattcac aacttctggg cggggtcgat aggcggcaca atgattgagg gtagttttct
    10441 gcttatgaat ggcttgaata tcattcgttt ctggcggatg cagaaaaggg gaattgatcc
    10501 gtttaaagta gagaaaaccc ctcccgccgt agacgaaagg ggttaaacaa ttaattacgc
    10561 agggcgctat ttgccagacg atcgtcttcc gcctggcaag ctgccgcagt gaacagcacg
    10621 tcagttgaag agttcagcgc ggtttcgcaa gagtcctgca atacgccgat gataaagccg
    10681 acggcaacca cctgcatggc gatatcgttc gaaataccga acatattaca ggccagcggg
    10741 atcagcagca gagaaccccc cgccacgccg gatgcgccac aggcacacag agaagccacc
    10801 acgctcaata gcagcgccgt gggcagatcg accgggatac ccagcgtatt aaccgcagcc
    10861 agcgtcaaca cggtaatggt gattgctgcg cccgccatat tgatggtggc acccagcgga
    10921 atagaaacgg aataggtatc gcgatccaga ttcagctttt cacacagtgc catattcacc
    10981 ggaatgttcg ccgcagagct gcgggtgaag aaggcataca cgccgctttc gcgcaggcac
    11041 agcagcacca gcgggaacgg gttacgacga attttccacc acaccagcaa tgggttaacc
    11101 accagcgcca ccagtaacat acagccaacc agcacgacca gcagttgcgc gtagccccac
    11161 agtgtggaga aaccggtggt tgccagggta gaagaaacca gaccaaaaat accaatcggt
    11221 gcgaagcgaa tgaccagttt caccataaag gtgacggcat tcgacatatc gttgaccagg
    11281 tttttggtgg tctcgttacc gtgacgcagt gcgaaaccga ggccaattgc ccacaccagg
    11341 atcccgatgt agttaccttt cagcagcgcg tcgatggggt tggaaaccat gctcattacc
    11401 agcccgcgca tcacttctac aatgcctgac ggtggcgaaa tatcacccgc gctactggat
    11461 aagtgcaggg tagaagggaa ggcgaagctg aaaacgactg cggccagagc agcagagaag
    11521 gtacccagca gatagaggaa caaaataggg cggatattgg ttttctgccc gtgctggtgg
    11581 ttagcaatag atgccatcac cagcatcaac accaggatgg gggcaacggc tttcagtgcg
    11641 ccgacgaaca aagtacctaa cagaccaaca gcttccgccg cgggttttga tatccatgcc
    11701 agaagaatcc ccagaacaag gccgaccagg atttgtttta ccaggctgcc atgagccaga
    11761 cgccggaata gccccggtga acgttgcgta gtcatttttc gatcctttca ttgtgttatc
    11821 gcacatccct ggtgcgttct gtcgacgcat ctttcaggat gtaacaaagt gtttgcaagg
    11881 tcgagtataa ggaaagttac agcttcggga agcgaaaaat gctggatttt aaagagaagt
    11941 catattttga aacttaatgg ttacatttgt ttgacataaa cagagggaag gactggctac
    12001 taaggctgat tagataacat atgggcaaaa ctatttggaa tatgagaacg gttgcgctcc
    12061 tttaatgtgc tatgccatat aaagtcactg g
//
LOCUS       DAZPDU010000130        11964 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZPDU010000130 DAZPDU010000000
VERSION     DAZPDU010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11964
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.131"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            1..326
                     /locus_tag="ACHURS_003200"
                     /pseudo
     CDS             1..326
                     /locus_tag="ACHURS_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
     gene            649..2037
                     /gene="narU"
                     /locus_tag="ACHURS_003201"
     CDS             649..2037
                     /gene="narU"
                     /locus_tag="ACHURS_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFR1238861.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIDDQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAITETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            2119..5859
                     /gene="narZ"
                     /locus_tag="ACHURS_003202"
     CDS             2119..5859
                     /gene="narZ"
                     /locus_tag="ACHURS_003202"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFR1238862.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDQPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKIYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGKETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5856..7400
                     /gene="narH"
                     /locus_tag="ACHURS_003203"
     CDS             5856..7400
                     /gene="narH"
                     /locus_tag="ACHURS_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFR1238863.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFKNTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7400..8095
                     /gene="narW"
                     /locus_tag="ACHURS_003204"
     CDS             7400..8095
                     /gene="narW"
                     /locus_tag="ACHURS_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFR1238864.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     ILSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            8092..8772
                     /gene="narI"
                     /locus_tag="ACHURS_003205"
     CDS             8092..8772
                     /gene="narI"
                     /locus_tag="ACHURS_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFR1238865.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYACFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRSLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8851..9744
                     /locus_tag="ACHURS_003206"
     CDS             8851..9744
                     /locus_tag="ACHURS_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFR1238866.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFELPLEGEVRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLCVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEI"
     gene            complement(9840..10685)
                     /gene="nhoA"
                     /locus_tag="ACHURS_003207"
     CDS             complement(9840..10685)
                     /gene="nhoA"
                     /locus_tag="ACHURS_003207"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HFR1238867.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQPMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10858..11427
                     /locus_tag="ACHURS_003208"
     CDS             10858..11427
                     /locus_tag="ACHURS_003208"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFR1238868.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVLITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSVREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11431..11658)
                     /gene="pptA"
                     /locus_tag="ACHURS_003209"
     CDS             complement(11431..11658)
                     /gene="pptA"
                     /locus_tag="ACHURS_003209"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HFR1238869.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIEPQMEALIKKPGYSM"
     gene            complement(11758..>11964)
                     /locus_tag="ACHURS_003210"
     CDS             complement(11758..>11964)
                     /locus_tag="ACHURS_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFR1238870.1"
                     /translation="WRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGL
                     RRKMRKAAMGRNYLASVLTGSGLS"
BASE COUNT         2842 a         3077 c         3113 g         2932 t
ORIGIN      
        1 gttatttaat ttgttttccc caacacttaa gcttaatact ggcctggcaa ttctttcgcc
       61 tggtgcattt gaagttcact ctgacggaat agatgtggat aacgaattgt ttcactatcc
      121 tattaaaaat agcatatacc ccatataata tacacactta caagacagag gaagttgtaa
      181 accagaggaa tataaaagtt aaaaacatga ccttagatga aataaacaat acttactgta
      241 ataacgatta ttacaatcag gcaataagag aggaaccgat agaccttctg gacagatcgt
      301 tttcctccag ttcatggcct ttttagtgaa taaatcatca ctaattctat aagtgtctgg
      361 tcttgttgat aatatagtta atatgtaacc ctttagattc acgatctgaa gggttgaaac
      421 atgacatacg ttcaaagatt aaccccgttt gaaggttagc ttgttagtta catttagtaa
      481 cacatatctg gctacttaat ccccattctg cccccgcggt tacgctttct tatctattct
      541 ttcaatcagc acttcaccca attcatcctt aattctaata actggctatt gcgccttacc
      601 tcctgttttg tcttttttca ttaccaatgt gtgcatgtga ggaacaatat ggcactgcaa
      661 aatgagaaaa atagtcgtta tcttttacgc gactggaaac cagaaaatcc ggccttctgg
      721 gaaaataaag gaaaacatat tgctcgaaga aacctctgga tatcagtcag ttgtctactt
      781 cttgccttct gtgtctggat gctatttagc gcagttaccg ttaatctcaa taaaatcggt
      841 tttaatttta ctaccgatca actcttttta ttaaccgcat taccctccgt ttctggcgca
      901 ttattgcgtg ttccctactc ctttatggtg cctatattcg gtggacgccg atggacggtt
      961 tttagtactg caatcctgat tattccttgc gtctggctcg gaattgccgt gcaaaatccg
     1021 aatactcctt ttgggatatt tatcgttatc gctttgctat gcggttttgc aggtgcaaac
     1081 tttgcttcga gcatgggcaa tatcagtttc ttctttccaa aagccaaaca agggagcgct
     1141 cttgggatta atggcggatt aggaaactta ggtgtaagtg taatgcagct ggttgcaccg
     1201 ctggtcattt ttgtacctgt atttgccttt ctcggcgtca atggcgtacc gcaggccgac
     1261 ggttcggtga tgtcgctggc gaatgccgca tggatttggg taccgctact ggcgattgcc
     1321 acgatcgccg cctggtcagg gatgaatgat atcgccagtt cacgcgcctc aattgacgac
     1381 cagctccctg tcttacaacg cctgcatctc tggctgctga gcctgcttta ccttgccacc
     1441 ttcggttcgt ttatcggttt ttctgcgggt tttgccatgc tggcaaaaac ccagttcccg
     1501 gatgtgaata ttctgcgcct ggcgttcttt ggcccattta tcggtgccat cgcgcggtcg
     1561 gttggtggtg ctatttccga taaattcggc ggcgtgcggg tgacgttgat caactttatt
     1621 tttatggcga ttttcagtgc cctgctgttc cttaccttac cgggcacagg ctccggtaat
     1681 ttcatcgcct tttacgccgt atttatgggg ctgtttctga ctgcgggtct gggaagtggt
     1741 tctactttcc agatgatcgc cgtcatcttt cgccagataa ccatttatcg ggtgaagatg
     1801 aaaggcggta gtgatgagca agctcagaaa gaagccatca ccgaaacggc ggcggctctg
     1861 ggctttatct cagccattgg cgcagtgggc ggctttttta ttccgcaggc gtttggcatg
     1921 tcgctcaata tgaccggctc tccggtcggc gcgatgaaag tgtttttaat cttctacatc
     1981 gtttgtgtgc tgctgacctg gctggtttat ggtcggcgga agttcagcca aaaataagag
     2041 atcgatgcct gatgtacaac cgtgtcgcat ccgacaaggc atcagatccc aattcattcc
     2101 tggagcagga gtcatgtcat gagtaaactt ttggatcgct ttcgctactt caaacaaaag
     2161 ggcgaaacct ttgccgatgg tcacggacag gtgatgcata gcaaccgcga ctgggaggac
     2221 agctatcgcc agcgttggca gttcgacaaa atcgtgcgtt ccacccacgg tgttaactgt
     2281 acaggctcct gtagctggaa aatctacgtt aaaaatggtc tggtgacctg ggaaatccaa
     2341 cagaccgact acccgcgcac tcgccctgac cagcccaatc atgaacctcg cggctgcccg
     2401 cgtggcgcaa gttactcctg gtatctctac agcgctaacc gcctgaaata cccgctcatt
     2461 cgtaaacgac tgattgaact gtggcgtgaa gctctcaagc aacacagcga tccggtactg
     2521 gcgtgggcat cgattatgaa cgatccgcaa aagtgcttaa gctacaaaca agtgcgtggg
     2581 cgcggcgggt ttatccgctc caactggcag gaacttaacc aactgattgc cgccgctaac
     2641 gtctggacca tcaaaatcta tggtccggat cgcgttgccg gtttctcgcc gatcccggcg
     2701 atgtcgatgg tgtcatacgc tgccggaacg cgttatctgt cgctgttagg cggcacctgt
     2761 ttaagcttct acgactggta ttgcgacctg ccgcccgcct cgccgatgac ctggggcgaa
     2821 caaaccgacg tgccggaatc tgccgactgg tataactcca gttacatcat cgcctggggg
     2881 tctaacgtac cgcagacacg tacgccggac gcccacttct ttaccgaagt acgttacaaa
     2941 ggcaccaaaa ctatcgccat tacccctgac tactctgaag tggccaaatt gtgcgaccag
     3001 tggctggcac cgaaacaagg cactgatagc gccctggcga tggcaatggg ccatgtgatt
     3061 ttaaaagagt ttcatctcga taatcccagc gactacttta tcaactactg ccgccgctat
     3121 agcgacatgc cgatgctggt aatgctggaa cctcgcgacg atggtagcta cgtacccggg
     3181 cgcatgatcc gcgcgtctga cctggtagat ggactaggcg aaagcaataa tccgcagtgg
     3241 aaaaccgtgg cggttaatac cgcaggtgaa ttggtagtgc cgaatggttc gattggtttc
     3301 cgctggggag aaaaaggcaa atggaatctg gaatccattg ccgccggtaa ggaaaccgaa
     3361 ttgtcgttaa ccctgctcgg tcaacatgac gctgttgcag gcgtggcctt cccctacttt
     3421 ggcggcattg aaaatccgca ttttcgcagc gtaaaacaca atccggtgct ggtgcgccaa
     3481 ttgcccgtta aaaacctgac gttagtcgat ggcaacacct gtccggtggt cagcgtttat
     3541 gatttggtgc tggcgaatta cggcctcgat cgcgggctgg aagatgaaaa cagcgcgaaa
     3601 gattacgctg aaatcaaacc gtacacccca gcctggggtg agcaaattac cggcgtaccg
     3661 cgccagtata ttgaaaccat cgcccgcgaa tttgccgata ccgcccataa aacgcatgga
     3721 cgctcaatga ttatcctcgg cgctggcgtc aaccactggt atcacatgga tatgaactac
     3781 cgtgggatga tcaatatgct gatcttctgc ggttgtgtcg ggcaaagcgg cggcggctgg
     3841 gcgcactatg tcggtcagga aaaactgcgc ccacaaaccg gctggttgcc gctggccttt
     3901 gcgctcgact ggaaccgccc accgcgccag atgaacagta cctcgttttt ctacaatcat
     3961 tccagccaat ggcgctatga aaaagtctct gctcaggagt tactttcacc gcttgccgat
     4021 gccagtaagt acagcggtca tctgattgat ttcaacgttc gcgccgaacg catgggctgg
     4081 ctaccttctg cgccgcagct ggggcgtaac ccgctcggga ttaaagctga agccgacaag
     4141 gccggattat cccccacaga atttaccgcc caggcgctga aatcgggcga tttacgtatg
     4201 gcctgcgaac aaccagatag cggcagcaat catccgcgta atttgtttgt ctggcgctct
     4261 aacctgcttg gctcctccgg caaaggccac gagtatatgc agaagtatct gctggggacc
     4321 gaaagcggga ttcagggcga ggaactcggt gccagcgacg ggatcaaacc ggaagaagtc
     4381 gagtggcaaa ctgcggcgat tgaaggcaag ctcgacctgc tggtgacgct cgacttccgc
     4441 atgtccagta cctgcctgtt ctccgatatc gttctgccca ccgccacctg gtacgaaaaa
     4501 gacgatatga acacctcgga tatgcatccg tttattcatc cgctttctgc ggcggtcgat
     4561 ccggcgtggg aatcacgcag cgactgggaa atctacaaag gtatcgccaa agcattttcg
     4621 caagtgtgcg tgggtcatct tggcaaagaa accgacgtgg tattacaacc cctactgcat
     4681 gactctccgg cagagctctc acagccgtgt gaagtgctcg actggcgcaa aggcgaatgc
     4741 gatctgattc caggcaaaac cgcgccgaat attgtggcgg tggagcgcga ctaccctgct
     4801 acgtatgaac gctttacctc gctcgggcca ttgatggaca aacttggcaa tggtggtaaa
     4861 gggatttcgt ggaataccca ggatgaaatc gatttcctcg gcaaactcaa ttacaccaag
     4921 cgtgatggcc cggcacaggg gcgtccgctg attgacaccg ccattgacgc ttcagaagtg
     4981 attctggcac tggcaccgga aaccaacggt catgttgcag tcaaagcgtg gcaggcgctg
     5041 ggcgagatca ccgggcgcga acatacccat ctggcgctgc acaaagagga cgagaagatt
     5101 cgctttcgcg atattcaggc gcagccgcgt aaaattatct ccagccccac atggtccggt
     5161 ctggaaagcg atcacgtctc ctataatgcg ggatacacca acgttcatga gttaattccg
     5221 tggcgcacgc tgtcgggacg ccagcagctc tatcaggatc atccgtggat gcgtgctttt
     5281 ggtgaaagcc tggtggctta tcgcccgcct atcgacaccc gtagcgtcag tgagatgcgc
     5341 cagatcccgc caaacggctt cccggaaaaa gcacttaact tcctgacgcc gcaccagaaa
     5401 tggggcattc actcaaccta cagtgaaaac ctgctaatgc tgacgctctc tcgcggtgga
     5461 ccgattgtct ggatcagcga aaccgatgcc cgagaactga ccattgtcga taacgactgg
     5521 gtggaagtct ttaacgccaa cggcgcgctg acggctcgtg cggtggtcag ccaacgtgta
     5581 ccgccgggta tgaccatgat gtatcacgcc caggaacgca ttatgaatat tcctggttcg
     5641 gaagtaactg gcatgcgcgg cggcattcat aactcggtca cccgtgtttg cccgaaaccg
     5701 acgcatatga ttggcggtta cgcgcagctg gcctggggct ttaactacta cggcaccgtc
     5761 ggctcgaacc gcgacgaatt catcatgatc cgcaagatga agaacgttaa ctggctggat
     5821 gatgaaggtc gcgatcaggt acaggaggcg aaaaaatgaa aatacgttcg caagtcggca
     5881 tggtgcttaa cctcgacaaa tgtatcggtt gccatacctg ttcggtgacc tgtaaaaacg
     5941 tctggaccgg acgcgaaggc atggagtacg catggtttaa caacgtcgaa accaaaccgg
     6001 gcattggtta tccgaaaaac tgggaagatc aggaagagtg gcaaggcggc tgggtccgcg
     6061 atgtgaatgg caagatacgc ccgcgtctgg gcagcaagat gggcgtaata accaaaatct
     6121 tcgccaaccc ggtggtgccg cagattgatg attactacga acctttcacc ttcgactacg
     6181 aacatttgca tagcgcaccg gaaggcaaac atatccctac tgctcgcccg cgttcgctga
     6241 ttgacggtaa gcggatggac aaagtgatct gggggccaaa ctgggaagaa ctgctgggcg
     6301 gcgagtttga aaaacgtgcc cgcgaccgca acttcgaggc catgcaaaag gagatgtacg
     6361 ggcagtttaa aaacaccttc atgatgtacc tgccgcgcct gtgcgaacac tgcctcaatc
     6421 ccagttgcgt ggcgacctgc ccaagcggcg ctatctacaa acgcgaagaa gacggcattg
     6481 tgctgattga tcaggataaa tgccgtggct ggcgtttgtg cattagcggt tgtccgtaca
     6541 aaaaaatcta cttcaactgg aaaagcggca agtcagaaaa atgtatcttc tgttatccgc
     6601 gaattgagtc aggccaaccc accgtgtgct cagaaacctg cgtgggtcgc atccggtatc
     6661 tgggcgtgct gctttacgac gccgaccgca ttgaggaagc ggcgagcacc gagcgcgaag
     6721 ttgacctcta tgaacgtcag tgcgaagtgt tcctcgatcc acacgatccc tcagtgatcg
     6781 aggaagccct gaaacaaggt attccacaaa acgtgattga agctgcccag cgttcgcctg
     6841 tctacaaaat ggcgatggac tggaaactgg cgctaccgct gcaccctgaa tatcgcaccc
     6901 tgccaatggt ctggtacgtt cctccgctgt cgccgattca gtcctacgca gatgcgggcg
     6961 gtttgccgaa aagcgaaggc gtgctgcccg ccatcgaaag cctgcgtatt ccggtgcaat
     7021 atctcgccaa tatgttgagt gccggcgata ccggtccggt actgcgggcg ctgaaacgga
     7081 tgatggcgat gcgccactat atgcgttcac aaaccgtgga aggcgttact gatactcgtg
     7141 ccatcgacga agtaggcctg agcgtcgccc aggtcgaaga aatgtatcgc tatctcgcca
     7201 ttgccaacta tgaagatcgt tttgttatcc cgaccagcca tcgggaaatg gcgggcgatg
     7261 ccttcgcaga acgcaacggc tgcggtttta cctttggcga cggttgccac ggttcggaca
     7321 gtaaattcaa cctgttcaac agtagccgta tcgatgccat caacatcacc gaagtgcgcg
     7381 acaaagcgga gggcgaataa tgcagatcct caaagtgatc ggcctgttga tggagtatcc
     7441 ggatgagctg ttgtgggaat gcaaggagga cgcgctggcg ttgatccgcc gcgacgcgcc
     7501 gatgcttacg gatttcaccc acaacctgct taacgcgccg ctgctggata aacaggccga
     7561 atggtgcgaa gtgtttgacc gcgggcgcac cacgtcgctg ctgctgttcg aacatgttca
     7621 tgccgagtcc cgcgatcgcg gccaggcaat ggtggacctg ctggcggagt atgaaaaggt
     7681 cggcctgcag ctggattgtc gggaactgcc cgattatcta ccgctgtatc tggagtattt
     7741 aagcgtactg cctgacgatc aggcgaaaga aggattgctc aatgttgcgc cgatcctcgc
     7801 cctgcttggc ggtcgcttaa aacaacgcga ggcaccgtgg tacgcgttgt ttgatgctct
     7861 gctgcaactg gcgggaagta tactttcaag tgacagtgtc acaaaacaag taaacagcga
     7921 agagcgcgat gacacccgcc aggcgcttga tgcagtgtgg gaagaggaac aggtgaagtt
     7981 tattgaagat aacgccacgg cgtgtgacag ctcgccgctt aatcaatatc agcgacgctt
     8041 tagccaggat gtcgcgccgc agtatgtcga catcagtgcg ggaggtggga aatgattcag
     8101 tatctgaacg tcttttttta cgatatctac ccgtacattt gtgcgacggt gtttttcctc
     8161 ggcagctggc tgcgctacga ctacgggcag tacacctggc gcgcctcctc aagtcaaatg
     8221 ctcgataaac gcgggatggt gatatggtcg aatctgttcc atatcggcat tttggggatt
     8281 ttcttcgggc acttgttcgg catgttaacg ccgcactgga tgtacgcgtg ttttttgcca
     8341 gtggcagcga aacaactcat ggcgatggtg ctcggcggta tttgcggtgt gttgacgttg
     8401 attggcggcg caggactgct gtggcgcagt ctgacaaatc agcgcgtgcg tgccacttcc
     8461 actacgccgg atatcatcat catgagcatt ttgctgatcc agtgtctgtt ggggctaagt
     8521 accataccgt tttcagcaca gtatcctgat ggtagcgaaa tgatgaagct ggtgggttgg
     8581 gcgcaaagca ttgtcacttt ccgtggtgga tcgtcagaga tgctcaacgg cgtagcgttc
     8641 gtcttccgcc tgcatctggt gttgggaatg accatcttcc tgctcttccc gttcacccga
     8701 ctggtgcatg tgtggagcgc gccgtttgag tactttactc gtcgatatca aattgtgcgt
     8761 tcgcggcgat aatttcaccc tggccggata gcccttcgct ctccggccac ttgcaggcta
     8821 tgatagttct ccagtcacag gagaacgtta atgaaaccgc aggtgtacca cgtcgatgct
     8881 tttacctctc aaccatttcg cggcaattct gccggtgttg ttttccccgc cgataatctt
     8941 agcgaagcac aaatgcagct tatcgcccgc gagttaggcc attcggaaac tgcttttctg
     9001 ctgcacagcg acgacagcga tgtgcgcatc cgctacttta cgccaacggt tgaagtgccg
     9061 atttgcggtc acgcgacagt agctgcgcac tatgtacgtg cgaaggtgtt aggtttagga
     9121 aattgcacga tctggcaaac atcgctggca ggaaaacatc gcgtgactat cgaaaagcac
     9181 aacgatgatt atcgtatttc gctggaacaa ggtacgccgg gctttgaact gccgctggag
     9241 ggtgaagtac gcgcggcgat tatcaacgca ttgcatctta ctgaagacga tattctgccg
     9301 ggtttgccga tccaggtcgc gacgaccggg cattctaaag tgatgatccc attgaagccg
     9361 gaagtggata tcgacgctct ttcgcctgac cttaatgcgc tgaccgctat cagtaaacag
     9421 attggctgca atggtttctt cccgttccag atccgtccag gcaaaaacga aaccgatggt
     9481 cgtatgttct cgcctgcgat tggtattgtg gaagatccgg tgactggcaa tgccaatggc
     9541 ccgatgggtg catggttggt acatcacaac gtattgcccc acgatggcaa cgtgttgtgt
     9601 gttaaaggcc atcaggggcg cgcattgggg cgtgacggca tgattgaagt gacggtgaca
     9661 attcgtgata accaaccgga aaaagtcacc atttcaggca cagcggtgat tttgtttcat
     9721 gccgaatggg caattgaaat ctgaaatcgt tgcctgatgc gcttcgcctg tcaggtctac
     9781 aaccgggcta atgtatgccg gataaggcgt ttacgccgca tccggcaacc tgacataaat
     9841 tattttcccg cctccggatg cgtatcaaac gccgccatca ccagcgctaa ctcatccaca
     9901 gtaaagccat gtttcgcatc atccacgccc agaccaaact gttcctgcat cacggcatat
     9961 aacgacgcca catccggtag atttcgctgc tccaccgcgt gcccattttc ataatgggta
    10021 aaatgaaaat tggtcagtgt cagcttgccg ccgtccggca aatggcggca catcagcaaa
    10081 tgatggcgaa aatgcgactg cggccagtgc gccgaccaga aattgcccat cacataatcg
    10141 ctttgttgct gctcgcagag atcgaagcgg tacatcggct gccaatgctg atgatgatta
    10201 aactgcaaca cccaatcatc accctcctgc aacaaccgat actctccgtg tggcgtggtc
    10261 tgcacgagat cggaaactaa acgaatcggc gcggttagcg tctgcccacc gaaaccgaca
    10321 tcagcaatcc atttttcccc ttccagttcc accaacagca aacgatgggt gcgcggcggt
    10381 aatgctggcg gatttgataa cactacgcgc cctaacaagc tgcgaacgtt aaaccccagc
    10441 tcgcgtaaca cccgctcaaa cacgccattc tgctcaaaac agtaaccgcc acgacgggct
    10501 atcaccagtt tctcttccag cgattgatca tcaagctgta tttccctcgg cagcaaaacg
    10561 tcaaggtttt caaacggaat ggtgcaattg tgtttcaggt gcaatgcacg aagcgtatca
    10621 atattgaccg cagcagctcc cgaccagtta atacgggcaa aatagtgatt cagaatgggc
    10681 gtcatcaggc gtttccctta gtggttttct caacttataa cctggaatag acgccgcacc
    10741 ggctattttg tgcgatcgcg gacatgcatc acgtttgcaa atcgccaatc ccgttaattt
    10801 cgttacttat tgcgttacgg actacgctta aaaaaacctt cttcgcgagg cgataccatg
    10861 agccgattca tccccataga attacaccat gccagccgtc tgttaaatca tggcccaact
    10921 gtcttgatca ccagtttcga tgagcaatcc cagcggcgta acattatggc tgcagcctgg
    10981 tcaatgccgg tggagtttga acctccacgc gtggcaattg tggtagataa atcgacatgg
    11041 accagggagt tgattgaacg taacggtaag tttggcatcg ttatcccggg cgttgcagca
    11101 actaactgga cgtgggcggt gggaagtgtg tcggtgcgtg aagaagataa atttaattgc
    11161 tatggcattc cggtggtgaa aggtccggtg cttggtttgc cacttgttga agagaaatgc
    11221 ctggcgtgga tggagtgccg cctgctacct gcgacttctg cgcaacaaaa atacgacacg
    11281 ctgtttggcg aggtggtatc agcagcggca gacgcacggg tatttgtcga aggccgctgg
    11341 cagtttgatg atgataagct caatacgttg catcatttag gtgctggcac gtttgttact
    11401 agcggcaagc gtgttacggc aggttaagca ttacatgctg taaccgggtt tctttatcaa
    11461 agcctccatt tggggctcga tttcggcatc ccagatagct tgccaagatt ctggttgaat
    11521 ctgctgtaga gcaatgctta tcgagctatc tttactgttc agatggcgaa taataacgtc
    11581 ggtaatatct gcagcaagtg ctgctttttg ttgttcgtcc agttcacgcg gaaagcattt
    11641 aatgtcgatg tgcggcatga ttacgctcct tttgaaagat gtcagtaaaa ttaacacaca
    11701 ttgtgaatgt catgtgtccg gtaaagcgtt tatacgacaa taatgcccag ggcaagatta
    11761 cgaaagcccg ctccccgtaa ggactgacgc caggtagttt ctgcccatgg ctgcttttcg
    11821 catcttacgt cttaaccctg ccttgaatac cttatcattc gtcaaaatat taatagcaat
    11881 gtgccgtatc cctgaaaata attctgctgc atttcctctt cttattttgc agtcgtcttc
    11941 attcattacc acgtccagac gcca
//
LOCUS       DAZPDU010000131        11954 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZPDU010000131 DAZPDU010000000
VERSION     DAZPDU010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11954
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.132"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..1112)
                     /gene="setA"
                     /locus_tag="ACHURS_003211"
     CDS             complement(<1..1112)
                     /gene="setA"
                     /locus_tag="ACHURS_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HFR1238871.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAV"
     gene            complement(1241..1345)
                     /gene="sgrT"
                     /locus_tag="ACHURS_003212"
     CDS             complement(1241..1345)
                     /gene="sgrT"
                     /locus_tag="ACHURS_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFR1238872.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLM"
     gene            1434..3089
                     /gene="sgrR"
                     /locus_tag="ACHURS_003213"
     CDS             1434..3089
                     /gene="sgrR"
                     /locus_tag="ACHURS_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFR1238873.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      3123..3222
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3253..4236
                     /gene="thiB"
                     /locus_tag="ACHURS_003214"
     CDS             3253..4236
                     /gene="thiB"
                     /locus_tag="ACHURS_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFR1238874.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFTFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVYPAFQNAIP
                     TGNWMYPVANVTQPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            4212..5822
                     /gene="thiP"
                     /locus_tag="ACHURS_003215"
     CDS             4212..5822
                     /gene="thiP"
                     /locus_tag="ACHURS_003215"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFR1238875.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMMCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQSVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     NVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            5806..6504
                     /gene="thiQ"
                     /locus_tag="ACHURS_003216"
     CDS             5806..6504
                     /gene="thiQ"
                     /locus_tag="ACHURS_003216"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFR1238876.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDSVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTDELL
                     SGKASASALLGITG"
     gene            complement(6574..7338)
                     /locus_tag="ACHURS_003217"
     CDS             complement(6574..7338)
                     /locus_tag="ACHURS_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR1238877.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWMAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(7424..8302)
                     /gene="araC"
                     /locus_tag="ACHURS_003218"
     CDS             complement(7424..8302)
                     /gene="araC"
                     /locus_tag="ACHURS_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFR1238878.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPPIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            8641..10341
                     /gene="araB"
                     /locus_tag="ACHURS_003219"
     CDS             8641..10341
                     /gene="araB"
                     /locus_tag="ACHURS_003219"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFR1238879.1"
                     /translation="MAIAIGLDFGSDSVRALAVECATGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKIVLAELSAEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATNAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            10352..11854
                     /gene="araA"
                     /locus_tag="ACHURS_003220"
     CDS             10352..11854
                     /gene="araA"
                     /locus_tag="ACHURS_003220"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFR1238880.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
BASE COUNT         2798 a         3351 c         3168 g         2637 t
ORIGIN      
        1 acagcaaagt gcccccaact ttgtgcaatt gctccctgaa taacgccagc cagaattacc
       61 ccggtagaaa tactgttagt aaataaggtg gtagctgccc ccgctcttcc aggcattaaa
      121 tcctgaaacc atagcatccc aatacccgca acaatgccga taaatacagc gttaaaaagt
      181 tgcagcgtca tcaacgccat acggctatga aagaaaatca atccggtgta aaacagtact
      241 cctgccgcca ctgctatgac catcattcgc cgcttaccat aacgtttgac atagtagcca
      301 gccagaatca ttgctggtat ttccagtcca gctgccgtcc ccatcaggaa acccgccagt
      361 ttgtctggca atcctaactc gctactgatc cacaacggca tatcaataat gtacatggtg
      421 ttgcaggtcc acattaacgt cgaggcgaca aataacatcc gtacgttact atcctgccag
      481 ccaccttgca ttgataaagc attttccgac ggcagttcta cccgcgccac agacggaagc
      541 ataaatgcaa tcaataccag actgagtgtg aatatcccgg cggcaatcga aaacatcacc
      601 gtaaagccgt aattcaacgc cagcataaag gccaacggtg gaccgataac ccatgccaga
      661 gaaagctgcg cacgcatcac cgagctaaac atcaccactt ctcgcgccga gttatccgca
      721 tattcccgcg ccagagcaaa taactgtggc attgccgtat tggccagaga tgccagaagc
      781 acaccacagg tgataagcgt cagataatga cgattaaatg caaacaatag cgcattgccg
      841 atagccatca aacagcaaaa tataatcagt tttcgccgat cgccctgact gtcagaacgt
      901 tttgccaacc agaggcttac gccgatccca gcaatagcat tcaccgtata aaagagtccg
      961 atccagaaag gttgcgcgcc aacctcacga ctcagaaata agctcaatgt aggagcctgt
     1021 agcgccccgg ccacccccat cataaaagcg accagcataa atgccgcgta aacaccgttc
     1081 atacggcgag ccatcgtcat tatccagatc atacgttccc tttttagcgc ggcgagaata
     1141 aaaaaaacca gcaggtataa tctgctggcg ggtgatttta caccaatact cagtcacaca
     1201 tgatgcaggc aagtcaactt tcagaattgc ggtcatccca ctacatcagt ccttcaagca
     1261 tggttaatcg ttgtgggacg cttaaccaac gcaaccagca caacttcgct gtcgcggtaa
     1321 aatagtgctg ataaaactga cgcatggggc acctccttgc ttcatcgtta ggaattattg
     1381 acttaatata gggaaaataa aattgctgtc ttttgcgcag gagttcccct tttatgccat
     1441 ctgctcgtct gcaacaacag ttcatccgcc tgtggcaatg ctgcgagggt aaatcgcagg
     1501 acacaacgct caacgaactg gcagcgttat tgagctgctc gcgtcgtcat atgcgtaccc
     1561 tgctcaacac catgcaggat cgcggctggc tgacgtggga agcggaagtc gggcgcggta
     1621 aacgctcgcg tctgacattc ctctataccg ggctggcgct tcagcaacag cgggcggaag
     1681 acctgctgga gcaggatcgt atcgaccaac tagtgcagtt ggttggcgac aaagcgactg
     1741 tgcggcaaat gctggtttct catctgggcc gcagcttccg ccaggggcgg cacatcctgc
     1801 gcgtgctcta ctatcgtccg ttgcgtaatc tgctacctgg cagcgcattg cgccgttccg
     1861 aaacccatat tgcccggcaa atcttcagtt ccctaacgcg cataaatgag gaaaatgggg
     1921 aactggaagc agacatcgcc caccactggc agcaaatatc accgcttcac tggcgtttct
     1981 ttttgcgtcc aggagtccat tttcatcatg gtcgtgaact ggaaatggac gacgtgatcg
     2041 cctctttaaa acgaatcaat acgctgccgc tctattcgca tattgcggac attgtgtcgc
     2101 cgacgccctg gacgctggat atccatctca cgcaaccgga ccgctggtta ccgttactgc
     2161 tggggcaagt tccggcgatg atcctgccgc gcgaatggga aaccctcagt aactttgcca
     2221 gccatcccat cggcaccggt ccgtatgcgg tgatccgcaa caccaccaat caactgaaaa
     2281 ttcaggcatt cgatgacttc ttcggttacc gggcattaat cgatgaagtt aacgtctggg
     2341 ttctgccgga aattgccgac gagccagctg gaggtctgat gctaaaaggt ccacagggtg
     2401 aggaaaaaga gattgaaagc cgcctggagg aaggttgcta ctatttacta ttcgacagcc
     2461 gcacccatcg cggggcgaat cagcaagtca gggactgggt aagctatgtg ctttctccaa
     2521 ctaatctggt ctatttcgct gaggaacagt accagcaact gtggttcccg gcttatggac
     2581 tgctcccccg ttggcaccat gcccgcacca taaagagcga aaaaccggct ggcctggaaa
     2641 gcctcaccct gaccttttat caggatcaca gtgagcatcg ggtgattgcc gggatcatgc
     2701 agcagattct ggcaagtcac caggtcacgc tggaaatcaa agagatcagc tacgatcagt
     2761 ggcatgaagg agagatcgag agcgatatct ggcttaacag cgccaacttt acgctgccgc
     2821 tggatttttc gctgttcgcg cacttgtgcg aggtgccgct gctccaacac tgtattccga
     2881 tcgactggca agccgacgcc gcccgctggc gcaatggcga aatgaacctg gcgaactggt
     2941 gccagcaact ggtcgccagc aaagcaatgg tgccgcttat ccaccactgg ctgatcattc
     3001 aggggcaacg cagtatgcgc ggcctgcgca tgaacaccct cggctggttc gattttaaat
     3061 cagcgtggtt tgcgccaccg gatccatgat tgctggtaga taacaaaatc actacactaa
     3121 cgccgttctc aacggggtgc cacgcaaacg cgtgcgctga gaaaataccc gtcgaacctg
     3181 atccggataa cgccgacgaa gggatttgag gctccttctc aagtcctttg ccactctttt
     3241 ttgaggtgca aagtgttaaa aaaatgtctt cccctgctgt tgctgtgcac agcgcccgtt
     3301 ttcgctaaac ccgttctgac tgtttatacc tacgattcct tcgccgccga ctgggggcct
     3361 ggtccaaaga tcaaaaaagc ttttgaagcc gactgtaact gcgaactgaa actggtggcg
     3421 ctggaagatg gcgtttcgct tctcaaccgt ctacggatgg aaggcaaaaa tagtaaagcc
     3481 gatgtggtgc tggggctgga taacaatctg ttagacgccg ccagtaaaac cggactattt
     3541 gccaaaagcg gtgtggcagc ggatgccgtt aacgttcccg gcggctggaa taatgacact
     3601 ttcgtcccgt ttgattacgg ctatttcacc ttcgtctatg acaagaacaa actgaaaaac
     3661 ccgccgcaaa gcctgaaaga attggttgag agcgatcaga actggcgggt gatttatcag
     3721 gatccgcgta ccagcacgcc ggggctgggt ctgctgttgt ggatgcaaaa agtctatggc
     3781 gatgacgccc cacaagcctg gcagaaactg gcgaagaaaa ctgtcacggt caccaaaggc
     3841 tggagcgaag cctatggcct gtttttaaaa ggcgaaagcg atctggtact gagttacacc
     3901 acctctccgg cttatcacat tctcgaagag aagaaagata actacgccgc cgcgaacttc
     3961 agcgaaggtc actatctgca ggtggaagtc gccgcccgca ccgctgccag caagcagccg
     4021 gagctggcgc aaaaattcct ccagtttatg gtttatccgg cttttcagaa tgcgatccca
     4081 accggcaact ggatgtatcc ggtggcaaac gtcacgcagc ctgccggttt tgaacaattg
     4141 accaaaccag caaccacgct ggagttcacg ccagccgaag tggcggcaca acgtcaggca
     4201 tggattagcg aatggcaacg cgccgtcagc cgttaattcc cggctggtta attccaggtg
     4261 taagcgccgc cacgctggtg gtagcggttg cgctcgcggc gtttctcgcc ctgtggtgga
     4321 acgcgccgca gggtaactgg gtagcagtct ggcaggacag ctacctgtgg catgtggtgc
     4381 gcttctcctt ctggcaggcg tttctctcgg cgctactctc tgtcgtaccc gcgatattcc
     4441 tcgcccgcgc cctctatcgc aggcgctttc cgggtcggct ggcgctgttg cgtctgtgcg
     4501 caatgacctt gatcctcccg gtgctggttg ctgttttcgg cattcttagc gtctatggtc
     4561 gtcagggctg gctggcatcg ctctgccaat cgctcggtct ggagtggacc ttttcgccct
     4621 acggcctgca aggcattttg ctggcccatg tgttttttaa tctgccgatg gcgagccgtt
     4681 tattacttca ggcactggaa aacattcccg gcgaacaacg tcaacttgcc gcccagcttg
     4741 gaatgcgtgg ctggcacttc tttcgtttcg tcgaatggcc gtggttacgg cgacaaatcc
     4801 cgccagttgc tgcgcttatc tttatgctct gtttcgccag cttcgccacc gtactgtcgc
     4861 taggcggcgg tccgcaagcg accactatcg agctggctat ttatcaggcg ctgagttacg
     4921 actacgatcc tgcccgcgcg gcgatgctgg cgctgatcca gatgatgtgt tgccttgggc
     4981 tggtgctgtt gagtcagcga ttgagtaagg ccattgcgcc cggcaccacg ctgctgcaag
     5041 gctggcgcga cccggacgat cgtctgcata gccgcatttg cgacacggtg ttaatagtgc
     5101 tggcgctgct gctgttgttg ccaccgttgc tggcggtgat cgtcgatggg gtaaatcgcc
     5161 agttgccgga agtgctggca caatcggtgc tgtggcaggc gctgtggacc tcgttgcgta
     5221 ttgcgctggc ggcaggtgta ttgtgcgtag tgctgaccat gatgctgcta tggagcagtc
     5281 gcgaacttcg ggcgcggcag aaaatgctgg cgggtcaggc gctggagatg agcggcatgt
     5341 tgatcctcgc catgccgggg attgtgctgg ctaccggctt ctttttactg ctcaacaaca
     5401 ctatcggcct gccacaatct gctgacggca ttgtgatttt caccaatgcg ttaatggcga
     5461 tcccttacgc gctgaaagta ctggaaaacc cgatgcgcga tatcaccgcc cgctacagca
     5521 tgttgtgtca gtcgttgggc attgaaggct ggtcacgctt aaacgtggtg gagctgcgcg
     5581 ccctgaaacg cccactggcg caggcgctgg cctttgcatg cgtgctgtcg attggtgatt
     5641 ttggcgtggt ggcgttgttc ggtaacgatg atttccgcac cctgccgttt tatctctacc
     5701 agcaaattgg ctcctatcgc agccaggacg gcgcggtcac cgcgttaatt ctgctactac
     5761 tctgttttct gctgtttacc gtgattgaaa aactaccggg gcgaaatgtt aaaactgact
     5821 gatatcacct ggctttacca ccatttgccg atgcgtttta gcttaacggt agaacgcggc
     5881 gagcaggtgg cgatcctcgg gccaagcggc gcaggtaaaa gtaccctgct gaatttgatt
     5941 gccggttttc tgacgccagc cagcggttcg ctgactatcg acagcgttga tcacaccacg
     6001 acgccgccgt cacgccgtcc ggtatcgatg ctgtttcagg agaacaacct gttcagccac
     6061 ctgacggtcg cgcagaacat cgggctgggg cttaatccgg gattgaagct gaacgcggcg
     6121 cagcaggaga aaatgcacgc tatcgcccgc cagatgggga ttgataattt aatggcgcgg
     6181 ttaccgggcg agctttccgg cggtcagcga cagcgcgtgg cgttagcgcg ttgtctggta
     6241 cgcgagcagc cgattttatt gctcgatgaa ccgttctctg cgctcgatcc ggcgctacgc
     6301 caggagatgt tgacgctggt gagcacgagc tgccagcagc aaaaaatgac gttattgatg
     6361 gtgtcgcaca gtgtggaaga tgccgcgcgg atcgccacgc gctcggtggt ggtcgccgac
     6421 gggcgcatcg cctggcaggg taaaaccgat gaattgttaa gcggtaaggc gagtgcttcg
     6481 gcgctgttgg gaattacagg ttaaaagcgc acggacagtc ccctcgcccc tctggggaga
     6541 agggaacaaa ccagcgccga tgcaaactat cccctaaacc ccaaccactt tacgcaaaat
     6601 atcgatatac accggcatca acgggtggcg aattaacacc accagcgcca ccacgccgat
     6661 ggcagaaatc aacggcgtca gccacaacaa acgaccgcgg gacaaataat gactcaaacg
     6721 gtcagtcgct ttaccactgc gccataaccg ccagcacagc cagccaccaa cccacaaaaa
     6781 taccgccgtc gccagcagca accatttaaa ctcaccgctc tgcatcccgg caggaatatc
     6841 gatcgccgcg cccgccagaa tccctggcag gaagtaaaac ggcggccaca gcaggcagcc
     6901 gataatattc ggcgtgataa attttgccac tggcagatcc agcattcccg ccaccattgg
     6961 caccagcgga cgcgtcgggc caacaaaacg accgaccaga atggtgaaca tgctgtgttg
     7021 atgcaacgca tgttcagttt tatcaagtag tgctttgttt ttcttcagaa atgaccagcg
     7081 atgcaacggc tttttaaaac gccagcccag ccagaaggag atccagtcgc ccagcaggca
     7141 accgacaatc cccgccatcc acgcgtgcca gaaacttaac tcgccgctgc caatcagcgc
     7201 tcccagcccc gccatcagca ccgtaccggg tagaatcaaa ccgaccagcg ccagcgactc
     7261 cagaaaggcc accaacacca ccgccatcaa tgaatacacg gtggattggg taataaagtg
     7321 ttccagcaat gcttgcataa tgtgcctgtc aaatggacga agcagggatt ctgcaaaccc
     7381 tatgctactc cgtcaagccg tcaattgtct gattcgttac caattatgac aacttgacgg
     7441 ctacatcatt cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc
     7501 attttttaaa tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg
     7561 tggcgatagg catccgggta gtgctcaaaa gcagcttcgc ctgactaatg cgttggtcct
     7621 cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg
     7681 gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat
     7741 cgctgatgta ctgacaagcc tcgcgtacac gattatccat cggtggatgg agcgactcgt
     7801 taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg
     7861 aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg
     7921 gctggtgcgc ttcatctggg cgaaagaaac ccgtattggc aaatattggc ggccagttaa
     7981 gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag
     8041 cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg
     8101 gtcggcagac aaattctcgc ccctgatttt tcaccacccc ctgaccgcga atggtgagat
     8161 tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg
     8221 cctcaatcgg cgttaaaccc gccaccagat gggcgttaaa cgagtatccc ggcagcaggg
     8281 gatcattttg cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt
     8341 gtccatattg catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaaccc
     8401 aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa
     8461 aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg
     8521 gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac
     8581 gctttttttc gcaactctct actgtttctc catacccgtt tttttggatg gagtgaaacg
     8641 atggcgattg caattggcct cgattttggc agtgattctg tgcgagcttt ggcggtggaa
     8701 tgcgctaccg gtgaagagat cgccaccagc gtagagtggt atccccgttg gcagaaaggg
     8761 caattttgtg atgccccgaa taaccagttc cgccatcatc cgcgtgacta cattgagtca
     8821 atggaagcgg cgctgaaaat cgtgcttgca gagcttagcg ccgaacagcg cgcagctgtg
     8881 gtcgggattg gcgttgacac aaccggctcg acgcccgcac cgattgacgc cgacggtaac
     8941 gtgctggcgc tgcgcccaga gtttgccgaa aacccaaacg cgatgttcgt attgtggaaa
     9001 gaccacactg cggttgaaga agcggaagag attacccgtt tgtgccacgc gccgggcaac
     9061 gttgactact cccgctatat tggcggtatt tattccagcg aatggttctg ggcaaaaatc
     9121 ctgcatgtga ctcgccagga cagcgccgtg gcgcaatctg ccgcatcgtg gattgagctg
     9181 tgcgactggg tgccagctct gctttccggt accacccgcc cgcaggatat tcgtcgcgga
     9241 cgttgcagcg ccgggcataa atctctgtgg cacgaaagct ggggcggctt gccgccagcc
     9301 agtttctttg atgagctgga cccgatcctc aatcgccatt tgccttcccc gctgttcact
     9361 gacacctgga ctgccgatat tccggtgggc accttatgcc cggaatgggc gcagcgtctc
     9421 ggcctgcctg aaagcgtggt gatttccggc ggcgcgtttg actgccatat gggcgcagtt
     9481 ggcgcaggcg cacagcctaa cgcactggta aaagttatcg gtacttccac ctgcgacatt
     9541 ctgattgccg acaaacagag cgttggcgag cgggcagtta aaggtatttg cggtcaggtt
     9601 gatggcagcg tggtgcctgg atttatcggt ctggaagcag gccaatcggc gtttggtgat
     9661 atctacgcct ggtttggtcg cgtactcggc tggccgctgg aacagcttgc cgcccagcat
     9721 ccggaactga aagcgcaaat caacgccagc cagaaacaac tgcttccggc gctgaccgaa
     9781 gcatgggcca aaaatccgtc tctggatcac ctgccggtgg tgctcgactg gtttaacggt
     9841 cgtcgcacgc caaacgctaa ccaacgtctg aaaggggtga ttaccgatct taacctcgct
     9901 accaacgctc cgctgctgtt cggcggtttg attgctgcca ccgcctttgg cgcacgcgca
     9961 atcatggagt gctttaccga tcaggggatc gccgtcaata acgtgatggc gctgggcggc
    10021 atcgcgcgga aaaaccaggt cattatgcag gcctgctgcg acgtgctgaa tcgcccgctg
    10081 caaattgttg cctctgacca gtgctgtgcg ctcggtgcgg cgatttttgc tgccgtcgcc
    10141 gcgaaagtgc acgcagacat cccatcagcc cagcaaaaaa tggccagtgc ggtagagaaa
    10201 accctgcaac cgcgcagcga acaggcacaa cgctttgaac agctttatcg ccgctatcag
    10261 caatgggcga tgagcgccga acaacactat cttccaactt ccgccccggc acaggctgcc
    10321 caggccgttg cgactctata aggacacgat aatgacgatt tttgataatt atgaagtgtg
    10381 gtttgtcatt ggcagccagc atctgtatgg cccggaaacc ctgcgtcagg tcacccaaca
    10441 tgccgagcac gttgttaatg cgctgaatac ggaagcgaaa ctgccctgca aactggtgtt
    10501 gaaaccgctg ggcaccacgc cggatgaaat caccgctatt tgccgcgacg cgaattacga
    10561 cgatcgttgc gctggtctgg tggtgtggct gcacaccttc tccccggcca aaatgtggat
    10621 caacggcctg accatgctca acaaaccgtt gctgcaattc cacacccagt ttaacgcggc
    10681 gctgccgtgg gacagtatcg atatggactt tatgaacctg aaccagactg cacatggcgg
    10741 tcgcgagttc ggcttcattg gcgcgcgtat gcgtcagcaa catgccgtgg ttaccggtca
    10801 ctggcaggat aaacaagccc atgagcgtat cggctcctgg atgcgccagg cggtctctaa
    10861 acaggatacc cgtcatctga aagtctgccg ttttggcgat aacatgcgtg aagtagcggt
    10921 caccgatggt gataaagttg ccgcacagat caagttcggt ttctccgtca atacctgggc
    10981 ggttggcgat ctggtgcagg tggtgaactc catcagcgac ggcgatgtta acgcgctggt
    11041 cgatgagtac gaaagctgct acaccatgac gcctgccaca caaatccacg gcgaaaaacg
    11101 acagaacgtg ctggaagcgg cgcgtattga gctggggatg aagcgtttcc tggaacaagg
    11161 tggcttccac gcgttcacca ccacctttga agatttgcac ggtctgaaac agcttccagg
    11221 tctggccgta cagcgtctga tgcagcaggg ttacggcttt gcgggcgaag gcgactggaa
    11281 aaccgccgcc ctgcttcgca tcatgaaggt gatgtcaacc ggtctgcagg gcggcacctc
    11341 ctttatggag gactacacct atcacttcga gaaaggtaat gacctggtgc tcggctccca
    11401 tatgctggaa gtctgcccgt cgattgccgt agaagagaaa ccgatcctcg acgttcagca
    11461 tctcggtatt ggtggtaagg acgatcctgc ccgactgatc ttcaataccc aaaccggtcc
    11521 agcgattgtc gccagcctga ttgatctcgg cgatcgttac cgtctgctgg ttaactgtat
    11581 cgacacggtg aaaacaccgc actccctgcc gaaactgccg gtggcgaatg cgctgtggaa
    11641 agcgcaaccg gatctgccaa ctgcttccga agcgtggatc ctcgctggtg gcgcgcacca
    11701 taccgtcttc agccatgcgc tgaacctcaa cgatatgcgc cagttcgccg agatgcacga
    11761 cattgaaatc acggtgattg ataacgacac ccgcctgcca gcgtttaaag acgcactgcg
    11821 ctggaacgaa gtgtattacg gatttcgtcg ctaagtagcc acatcaggta tgtaacgcct
    11881 gatgcgacgc tgacgcgtct tatctggcct acacgctgcg attttgtagg ccggataagc
    11941 aaagcgcatc cggc
//
LOCUS       DAZPDU010000132        11939 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZPDU010000132 DAZPDU010000000
VERSION     DAZPDU010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11939
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.133"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..914)
                     /gene="ydcO"
                     /locus_tag="ACHURS_003221"
     CDS             complement(<1..914)
                     /gene="ydcO"
                     /locus_tag="ACHURS_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFR1238881.1"
                     /translation="MRLFSIPPPTLLAGFLAVLIGYASSAAIIWQAAIVAGATTAQIS
                     GWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLTLNEAIGVFIVTNA
                     LIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQFTLCGSMLLVWLA
                     TKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPDFSFAHSLSVALPL
                     FLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVYSVGIAAITAAICQ
                     SPEAHPDKDQRWLAAAVAGIFYLLAGLFG"
     gene            1006..1542
                     /gene="sutR"
                     /locus_tag="ACHURS_003222"
     CDS             1006..1542
                     /gene="sutR"
                     /locus_tag="ACHURS_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFR1238882.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSAMPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            1615..3576
                     /gene="rlhA"
                     /locus_tag="ACHURS_003223"
     CDS             1615..3576
                     /gene="rlhA"
                     /locus_tag="ACHURS_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFR1238883.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLNV
                     AVTEPLANGDGLNVMIKREVIGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVFPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(3668..3898)
                     /locus_tag="ACHURS_003224"
     CDS             complement(3668..3898)
                     /locus_tag="ACHURS_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HFR1238884.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            4120..4274
                     /locus_tag="ACHURS_003225"
                     /pseudo
     CDS             4120..4274
                     /locus_tag="ACHURS_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase"
     gene            4320..4736
                     /gene="hicB"
                     /locus_tag="ACHURS_003226"
     CDS             4320..4736
                     /gene="hicB"
                     /locus_tag="ACHURS_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFR1238885.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            4815..6221
                     /locus_tag="ACHURS_003227"
                     /pseudo
     CDS             4815..6221
                     /locus_tag="ACHURS_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
     gene            6466..7611
                     /gene="ydcS"
                     /locus_tag="ACHURS_003228"
     CDS             6466..7611
                     /gene="ydcS"
                     /locus_tag="ACHURS_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFR1238886.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            7629..8642
                     /locus_tag="ACHURS_003229"
     CDS             7629..8642
                     /locus_tag="ACHURS_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1238887.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKI
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            8643..9543
                     /locus_tag="ACHURS_003230"
                     /pseudo
     CDS             8643..9543
                     /locus_tag="ACHURS_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            9533..10327
                     /locus_tag="ACHURS_003231"
     CDS             9533..10327
                     /locus_tag="ACHURS_003231"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1238888.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGSVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     LLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            10349..11773
                     /gene="patD"
                     /locus_tag="ACHURS_003232"
     CDS             10349..11773
                     /gene="patD"
                     /locus_tag="ACHURS_003232"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFR1238889.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
BASE COUNT         2884 a         2897 c         3203 g         2955 t
ORIGIN      
        1 ccaaacagac ctgcgagcaa atagaaaatg cctgcaacgg cagctgccag ccaacgttga
       61 tctttatccg gatgcgcttc cgggctttgg caaatagccg cggtgattgc cgcaataccg
      121 acggaataaa cgccgaaagg ggaaaaaacc agtgccagca atccagtaaa tacaattaat
      181 ggcgaaacag gagccgaata tccagctgct ttcattgctg cgatacccgg tgcgttttgc
      241 gatgccatcg tcaccagaaa aagggggagt gcaacgctca ggctgtgagc aaacgaaaaa
      301 tcaggggtaa tataagtggg gagaacgggt ttaaagacaa catcagttgt gacaacgtca
      361 ccttgcgcga taacgatcac gatcccaata atcatcgcgg caattaccgc atagcgcggc
      421 gcaacggcct tggttgccag ccataccagc aacatacttc cacacaacgt aaattgaccg
      481 tccagactgg caaacgcctg taaaccaaag cgtaataaaa tcccggcaag cattgccgcc
      541 gcaagcgagt gcggaataat gcgcatcaga cgagcaaaga gtcccgttat gccgcagagg
      601 actattaacg cgttggtgac aataaaaacg ccgatggctt cgttaagtgt tagtccctgc
      661 aatccggtga ccaacaaagc cgcgccaggc gttgaccatg cggtgagaac aggtacgcga
      721 taccataatg tcagagtcag cgtactgacg cccattgcca gccccagcgc cgtcatccag
      781 ccagagattt gtgcagtggt ggctccggcg acaatcgctg cttgccagat tattgccgct
      841 gaactggcgt agccaattaa taccgccaga aaccccgcca gtagcgtggg tggaggaata
      901 gaaaacagac gcatagtcac ctcgtgcgtt atagcgtaca ggcaccgtag catttgtccg
      961 ttataacgca caagtgataa acttccgttt tgccggagga gtcgcatgga aaatctcgct
     1021 cgctttttat ccactacact taaacaacta cgccagcagc gcggctggag tctttcgcga
     1081 ctggcagaag cgacgggcgt ttctaaagca atgttagggc agattgagcg taatgagtcc
     1141 agtccgacgg tcgcgacatt atggaaaatt gccaccgggt tgaacgtacc attttcaaca
     1201 tttatttctc cgccgcagtc cgctatgcct tcagtttatg atccacaaca gcaggcgatg
     1261 gtgatcactt cgctgtttcc ttatgatcca caactctgct ttgaacactt ctcgattcag
     1321 atggcatctg gtgcaataag cgaatcgacg ccccatgaga agggggtaat cgaacatgtc
     1381 gtcgtcattg atggacaact tgatctgtgc gttgatgggg aatggcagac ccttaattgt
     1441 ggggaaggcg ttcgatttgc tgcagacgtc acgcatatct atcgtaatgg tggggagcaa
     1501 accgtacatt ttcattccct catccattac ccgcgcagtt aagcgggaaa actatttcgc
     1561 aacgtcgcgc ttgtggctaa aatagccgcc atttttcagc tactggataa gaatgtgacc
     1621 gtatcttctc atcgacttga actgttaagc ccggcacgcg atgccgccat tgcccgcgaa
     1681 gctattttgc acggtgccga tgctgtttat atcggcggcc ctggttttgg tgcccgtcat
     1741 aatgccagta atagcctgaa agatattgcc gagctggtgc cgtttgccca tcgttatggt
     1801 gcaaaaattt tcgtcacgct taacaccatt ttgcatgatg atgagctgga acccgcgcaa
     1861 cggctgatta ctgacctcta ccagaccggt gtcgatgcgc tgattgttca ggatatgggg
     1921 attctggaac ttgatattcc gccgattgaa ctgcacgcca gtacgcagtg cgacattcgt
     1981 acagttgaaa aagcgaagtt cctctctgat gttggcttca cgcagattgt gctggcgcga
     2041 gagctgaatc ttgatcagat ccgcgcgatt caccaggcta cggacgcgac cattgaattc
     2101 tttattcatg gggcactgtg cgtggcctat tcgggtcagt gctacatttc tcatgcgcaa
     2161 acagggcgta gcgccaaccg tggcgattgc tcgcaggcgt gccgtttgcc atacacattg
     2221 aaagacgatc aggggcgggt ggtttcctat gaaaaacatc tgctgtcgat gaaagataac
     2281 gatcagactg ctaacctcgg cgcgctgatt gatgctggcg tacgctcctt caagattgaa
     2341 gggcgttaca aagatatgag ctacgtgaag aatatcaccg cccattatcg ccagatgctg
     2401 gatgccatta ttgaagaacg tggcgatctg acgcgtgctt catcaggtcg tactgaacat
     2461 ttctttgttc catcgacgga aaagactttc caccgtggta gcacagatta ttttgtgaat
     2521 gcccgtaaag gcgatattgg cgcgttcgat tcgccgaaat ttatcggcct gccggtgggt
     2581 gaagtattga aagtggcgaa agatcatctc aatgttgccg tcaccgagcc actggcaaat
     2641 ggtgatggcc tgaacgtgat gattaaacgt gaagtcatcg gttttcgtgc caatacggtc
     2701 gagaaaaccg gagaaaatca gtaccgcgtc tggcccaatg aaatgccagc agatttgcac
     2761 aaaattcgtc cacatcaccc actaaaccgt aatcttgatc ataactggca gcaggcactg
     2821 acaaaaacct ctagcgaacg tcgggtggcg gtagacattg aactgggcgg ctggcaggaa
     2881 caactgattc tgaccctcac cagtgaagag ggtgtcagca tcacgcatac gctggacggg
     2941 cagttcgacg aagccaataa cgccgaaaaa gcaatgaaca atctgaagga tggtctggca
     3001 aaactggggc aaaccctcta ttacgcccgc gatgtgcaaa ttaatttgcc gggggcgctg
     3061 tttgtaccaa acagtctgtt aaaccagttc cgccgtgaag ctgctgacat gctggatgct
     3121 gcgcgtcttg ccagttacca gcgcggcagc cgtaaaccgg ttgctgatcc tgcgccggtt
     3181 tttccgcaaa cgcatctgag tttcctcgcg aacgtataca accagaaagc gcgtgaattt
     3241 tatcatcgct atggtgtgca gctgattgac gcggcgtatg aggcacatga agagaagggc
     3301 gaagtcccgg tgatgatcac caagcattgt ctgcgctttg cctttaatct gtgcccgaaa
     3361 caggcgaaag gcaatatcaa aagctggaag gcgacgccaa tgcaactggt taacggcgat
     3421 gaagtattaa cgctaaagtt tgattgccgc ccatgcgaga tgcacgtcat tggcaaaatc
     3481 aaaaatcaca tactgaaaat gccgttaccg ggaagcgtag tggcatccgt aagtccggat
     3541 gagctgctga aaacattgcc taagcgaaaa gggtaaaacg ccagttttct ggttactcac
     3601 aactcattga atctgcatga tattgcctgc cgggtaaggc gtcacgccgc atccggcatc
     3661 aaacgactca acgttgtccc gcttctggtt tgcgcgattt ttgccagtaa aaatgctcgc
     3721 gcaaaccttc cgccgactct tccgccacag cacgcaattc atcgctgtcc gcttcatgac
     3781 gcagctgatg atccacattc tttacccaca caaattcatg tcctttgtgc cctgccatga
     3841 gttgtcctga gaacagcgca cacgttaata agacaaccga taacgccttc gtaaacatcc
     3901 tgccaccttt ttcttacctt ttgccgctat gatgccgatc gtttcttgag gttattattc
     3961 agttttgcaa attagcgcaa agaaattctg gaatcttcct tcctgatttt gcattgcatt
     4021 ctgccgttgc ggcgatttag tgctattttc gagcaaatta cacatagggg taaacactaa
     4081 tgtttacttt attgatatac tcagcggcag ggaggcgatg tgaaacaaag cgagttcaga
     4141 cgttggctcg aatctcaggg cgtcgatgta gcgaatggcc gcaaccattt gaaactcagg
     4201 tttcatggga ggcgcagtgt catgccgcgt cactcctgcg taaagcaatc ctgaaacaac
     4261 tcggtttgag ttaatcgcca attaaaaagg ttaatgacat gcgagagaca gtcgaaatta
     4321 tgcgttatcc cgtcactctt acacccgcgc cggaaggcgg ttatatggtt tcttttgtgg
     4381 atatccctga agcgttgacg cagggcgaaa ctgtcgctga agcgatggaa gcggcaaaag
     4441 atgctttact gaccgcattt gatttttatt ttgaagataa cgagcttatc ccgttacctt
     4501 cgccattaaa tagtcatgat cactttattg aagtaccttt gagcgtcgcc tctaaagtat
     4561 tgctgttaaa tgctttttta cagtcagaaa tcactcagca agagttagcc aggcgaattg
     4621 gcaaacctaa acaggagatt actcgcctat ttaacttgca ccatgcgaca aaaatcgacg
     4681 ccgtccagct tgcggcaaag gcgcttggca aagagttatc gctggtgatg gtttaattac
     4741 agttaacgaa aagttgtcat ttttaacaac tgatatagac tgccgaatca tctgcacata
     4801 attacgattc gataatgaaa aaataccagc agcttgcaga acaattacgc gagcagattg
     4861 cgtcgggtat ctggcaaccc ggcgatcgtt tgccttcgtt gcgtgaccag gtggcgcttt
     4921 caggcatgag ctttatgact gtcagccatg cctatcagtt gctcgaaagt cagggatata
     4981 ttatcgcacg accgcagtcg ggttattacg ttgcgccaca ggcaataaaa atgccgaaag
     5041 cgccagtcat tccagtcact tgagatgaag cagtcgatat caacacttat atttttgata
     5101 tgttgcaggc cagtcgcgaa ccgtcggtcg ttccgtttgc ctcggccttt cccgacccgc
     5161 gacttttccc cctccaacaa ctaaaccgct cgctggcgca ggtaagcaaa accgccacag
     5221 cgatgagcgt gattgaaaac ttaccgccag gaaacgcaga actgcgtcag gctattgctc
     5281 gtcgctatgc cttacagggc atcaccattt ctcctgatga aattgtcatt actgccgggg
     5341 cgttagaggc attaaacctc agtttgcaag cggtaactga accgggcgat tgggtgatag
     5401 tagagaatcc ttgtttctac ggtgcgttgc aggcgctgga gcggctacgg ctgaaggcgt
     5461 tatcggtggc gacggatgtt aaagaaggga tcgatcttca ggcgctggaa ctggcgttgc
     5521 aggattatcc ggtgaaagcg tgctggctga tgactaatag ccagaaccca ctcggattta
     5581 ccttaacgcc gcaaaaaaaa gcacaactgg tggcgttact caatcagtac aacgtaacgc
     5641 tgattgaaga tgacgtttac agcgaacttt attttggacg ggaaaaaccg ctgcctgcga
     5701 aagcgtggga tcgccacgat ggcgttttgc attgctcttc gttttcgaaa tgtctggtgc
     5761 ctggttttcg tattggttgg gtcgccgccg gaaaacatgc acgtaaaatt caacgcttgc
     5821 agttgatgag tacgctttcc accagctcac cgatgcaact tgcgctggtg gattaccttt
     5881 ccacgcgccg atacgacgcc catcttcgtc gcctgcgtcg ccagcttgcg gaacgtaaac
     5941 aacgtgcctg gcaggcactg ctgcgttatc tgcctgcgga agttaaaatt catcatagtg
     6001 acagcggtta ttttctctgg ctggagctcc ccgagccgtt agatgccggc gaattaagcc
     6061 tggcggcact gacgcatcat atcagtattg cgccgggtaa aatgttttct accggtgaaa
     6121 actggtcacg tttttttcgt tttaataccg cgtggcagtg gggagaacgt gaagaacagg
     6181 cggtaaaaca attaggcaaa cttattcaag aacggctgta atagcgttta atttaattcc
     6241 tcttagattg ggtaatatga atttcgaata gcagtcatat ttcctaaccc cttgtctata
     6301 ctccagaaga taaccttaca gacggcataa tgcgcggtag ctcacaacct gattaaattt
     6361 tctcagggac gaaggtgtgc ctgcaagccg ccgtctatgg ttaaacaagg agatattttt
     6421 acggcacggc ggctgaacaa ttaattacga caggagtaag accttatgag caagacattt
     6481 gcccgcagca gcctgtgtgc gctcaccatg acaataatga ccgctcacgc cgccgaacca
     6541 cctaccaatt tagataaacc ggaagggcga ctggatatta tcgcctggcc gggatacatc
     6601 gaacgcggac aaactgataa acaatacgac tgggtaacgc aatttgaaaa agagacaggc
     6661 tgcgcggtga atgtgaaaac cgccgcgact tccgatgaaa tggtcagtct gatgaccaaa
     6721 gggggttacg atctggttac ggcatccggc gatgcctcgc tgcgtttgat tatgggcaaa
     6781 cgcgtgcagc cgattaatac cgccttgatt cccaactgga aaacgctcga tccgcgcgtg
     6841 gttaaaggcg actggtttaa tgttggcggc aaagtttacg gcacacctta ccaatggggg
     6901 ccgaacctgc tgatgtacaa cactaaaacc ttcccgacgc cgccggatag ctggcaagtg
     6961 gtttttgttg agcaaaatct gccggacggc aagagcaata aaggccgcgt tcaggcttat
     7021 gatggcccta tctacattgc ggacgctgcg ttgttcgtta aagccactca gccgcagttg
     7081 ggcatcagcg atccgtatca actaaccgaa gaacagtacc aggcggtgct gaaagtgctg
     7141 cgcgctcaac acagtttgat ccatcgctac tggcatgaca ctaccgtgca aatgagcgat
     7201 ttcaaaaacg agggtgtggt tgcttccagt gcctggccct atcaggccaa cgccctgaaa
     7261 gccgaaggcc agcctgttgc taccgttttc ccgaaggagg gtgttaccgg ttgggctgat
     7321 accaccatgc tgcatagcga agcgaaacat ccggtttgcg cctacaaatg gatgaactgg
     7381 tcattaacgc caaaagtgca gggcgatgtg gcggcctggt ttggctcgtt accggtagtg
     7441 ccggaagggt gtaaagccag tccgttatta ggcgaaaaag gttgtgaaac caacggtttt
     7501 aactatttcg acaaaatcgc cttctggaaa acgcctatag cagaaggggg caagtttgtt
     7561 ccctacagtc gctggacgca ggattacatt gccattatgg gcggtcgcta acttcgctgg
     7621 ggtgctttat gacgtacgca gtggagtttg acaacgtctc gcggttgtac ggtgacgtgc
     7681 gcgcagtaga tggcgtcagt attgcgataa aagatggtga gttcttctct atgctggggc
     7741 cgtccggctc cggcaaaatc acctgcctgc gcctgattgc tggcttcgaa cagctttccg
     7801 gcggggctat ctctatcttt ggtaaacccg ccagcaatct gccaccgtgg gagcgggacg
     7861 tgaatactgt ctttcaggac tacgcgctat ttccgcatat gtcgattctt gacaatgtcg
     7921 cctatgggct gatggtcaaa ggcgtgaata aaaagcagcg gcacgcaatg gcgcaagagg
     7981 cgctggagaa agtggcgttg gggtttgtac agcaacgtaa accgtcacaa ctttctggtg
     8041 gtcagcgcca gcgggttgct atcgccagag cattggtgaa tgaaccgcgc gtattgctgt
     8101 tggatgaacc gctcggcgca ctggatctca aattgcgtga gcagatgcag ctggaactga
     8161 aaaaactgca acagtctctc ggtatcactt ttatcttcgt tacccacgat cagggcgaag
     8221 cgttatcgat gtccgatcgt gtggcggttt tcaataatgg acgcattgag caggtcgatt
     8281 ccccgcgcga tctctatatg cgcccgcgca cgccgtttgt tgccgggttc gttggtacat
     8341 cgaatgtttt tgatggactg atggcagaga aactttgtgg catgacggga agcttcgccc
     8401 tgcgaccgga acatatccgc ctcaacaccc ctggtgaact gcaggccaat ggcacgatcc
     8461 aggcggtgca atatcagggc gcggcaactc gttttgaact gaaattgaac ggcggtgaaa
     8521 aactgcttgt gagtcaggcc aatatgacag gcgaagaact gcctgccacg ctcacgcccg
     8581 gacaacaggt gatggtttcc tggtcgcgtg atgtgatggt gccgctggtt gaggagaggt
     8641 gaatggcgat gaatgtattg caatcacctt cacgtccagg tctggggctg tttttactgc
     8701 tgcttggccc gctaatgtgg tttggcattg tctatttcgg ctcgctgctg acactgttat
     8761 ggcagggatt ttatactttt gacgatttca ccatgtcggt aacgccggaa ctgacgctgg
     8821 cgaatatccg tgcgctgttt aatccggcga attacgacat cattctccgc acgctgacta
     8881 tggctgtggc agtcactatc gccagcgcca ttctggcttt tccaatggcg tggtatatgg
     8941 cgcgctatac cagcgggaaa atgaaagcgt ttttatattg cggtaatgtt gccgatgtgg
     9001 gcgagctaca ttgttaaagc ctatgcctgg acgttattgc tggcaaaaga tggcgtggct
     9061 cagtggtttt tacaacatct tgggctggaa ccactgctga ctgcgttcct tacattacct
     9121 gcggtgggcg gaaatacgct gtcaacttcc gggctggggc gctttctggt gtttctctat
     9181 atctggttgc cgttcatgat cctgcccgtt caggcggcgc ttgagcgttt gccgccgtca
     9241 ttgttgcagg cgtcggctga tctcggcgca cgtccacgac aaacctttcg ctatgtggtg
     9301 ctgccgctgg caatcccggg tattgccgct ggctctatct ttaccttctc actcacactg
     9361 ggcgatttta tcgtcccgca gctggttggt cctccaggat attttatcgg caatatggtt
     9421 tattcccagc agggggcgat tggcaatatg ccgatggcgg cggcattcac cctggtgccg
     9481 attattctca tcgcactgta cctggcgttc gtgaaacgtc tgggagcgtt cgatgcactc
     9541 tgaacgcgca ccgtttttcc tcaaactggc ggcctggggc ggcgttgttt tcctacattt
     9601 tcccatcctg ataatcgccg cctatgcgtt taacactgaa gatgcggcgt ttagttttcc
     9661 accgcagggc ctgacgctgc gctggtttag cgtggcagca cagcgtagtg atattcttga
     9721 tgccgtgaca ctgtcactta aagtggcggc gctggcgaca ttaattgcgc tggtgttagg
     9781 gacgctggca gctgccgcgc tgtggcgacg agactttttc ggcaaaaacg ccatttcgct
     9841 gttactgctg ctgcccattg cgctgccggg cagcgtcact ggtctggcgt tattaaccgc
     9901 ctttaaaacc atcaatctgg agccgggatt tttcaccatc gtggtcggtc atgcgacttt
     9961 ttgtgtagtt gtggtgttta acaatgtcat cgcccgtttt cgccgcacct cctggagtct
    10021 ggttgaggcg tcaatggatc ttggggccaa tggctggcaa accttccgct acgtagtgtt
    10081 gccgaatctc agttcggcgt tactggcagg aggaatgctg gcgtttgcct tgtcgttcga
    10141 tgaaatcatc gttacgacct ttacggcagg tcatgaacga acgttactgt tgtggttgct
    10201 caatcagctt gggcgaccgc gtgatgtacc ggtaactaac gtggtggcac tgctggttat
    10261 gttggtaaca accttgccga tcctgggggc ctggtggcta acccgcgaag gcgacaatgg
    10321 tcaataacca ctgatacagg aatatgctat gcaacataag ttactgatta acggagaact
    10381 ggttagcggc gaaggggaaa aacagcctgt ctataatccg gcaacggggg acgttttact
    10441 ggaaattgcc gaggcatccg cagagcaggt cgatgctgct gtgcgcgcgg cagatgcagc
    10501 atttgccgaa tgggggcaaa ccacgccgaa agtgcgtgcg gaatgtctgc tgaaactggc
    10561 tgatgttatc gaagaaaatg gtcaggtttt tgccgaactg gagtcccgta attgtggcaa
    10621 accgctgcat agtgcgttca atgatgaaat cccggcgatt gtcgatgttt ttcgcttttt
    10681 cgcgggtgcg gcgcgctgtc tgaatggtct ggcggcaggt gaatatcttg aaggtcatac
    10741 ttcgatgatc cgtcgcgatc cgttgggggt cgtggcttct atcgcaccgt ggaattatcc
    10801 gctgatgatg gccgcgtgga aacttgctcc ggcgctggcg gcagggaact gcgtagtgct
    10861 taaaccatca gaaattaccc cgctgaccgc gttgaagttg gcagagctgg cgaaagatat
    10921 cttcccggca ggcgtgatta acatactgtt tggcagaggc aaaacggtgg gtgatccgct
    10981 gaccggtcat cccaaagtgc ggatggtgtc gctgacgggc tctatcgcca ccggcgagca
    11041 catcatcagc cataccgcgt cgtccattaa gcgtactcat atggaacttg gtggcaaagc
    11101 gccagtgatt gtttttgatg atgcggatat tgaagcagtg gtcgaaggtg tacgtacatt
    11161 tggctattac aatgctggac aggattgtac tgcggcttgt cggatctacg cgcaaaaagg
    11221 catttacgat acgctggtgg aaaaactggg tgctgcggtg gcaacgttaa aatctggtgc
    11281 gccggatgac gagtctacgg agcttggacc tttaagctcg ctggcgcatc tcgaacgcgt
    11341 cagcaaggca gtagaagagg cgaaagcgac agggcacatc aaagtgatca ctggcggtga
    11401 aaagcgcaag ggtaatggct attactatgc gccgacgctg ctggctggcg cattacagga
    11461 cgatgccatc gtgcaaaaag aggtatttgg tccagtagtg agtgttacgc ccttcgacaa
    11521 cgaagaacag gtggtgaact gggcgaatga cagccagtac ggacttgcat cttcggtatg
    11581 gacgaaagat gtgggcaggg cgcatcgcgt cagcgcacgg ctgcaatatg gttgtacctg
    11641 ggtcaatacc catttcatgc tggtaagtga aatgccgcac ggtgggcaga aactttctgg
    11701 ttacggcaag gatatgtcac tttatgggct ggaggattac accgtcgtcc gccacgtcat
    11761 ggttaaacat taagttagat gttagtggct gttataggac ggccactatc gttttaggtt
    11821 ctgagtttaa tggcggttaa gtattagaat gattacatta ataactagcg taaaaattag
    11881 gctaaatgtt tgatggcgat ttttcatcct gtcacagttg attagcataa ggctttaga
//
LOCUS       DAZPDU010000133        11821 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZPDU010000133 DAZPDU010000000
VERSION     DAZPDU010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11821
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.134"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            50..616
                     /locus_tag="ACHURS_003233"
     CDS             50..616
                     /locus_tag="ACHURS_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1238890.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            772..2804
                     /locus_tag="ACHURS_003234"
                     /pseudo
     CDS             772..2804
                     /locus_tag="ACHURS_003234"
                     /inference="COORDINATES: protein motif:HMM:NF012786.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            2781..3125
                     /locus_tag="ACHURS_003235"
     CDS             2781..3125
                     /locus_tag="ACHURS_003235"
                     /inference="COORDINATES: protein motif:HMM:NF025319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC C-terminal domain-containing protein"
                     /protein_id="HFR1238891.1"
                     /translation="MNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGKDVNVIIRNA
                     SGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQHCSLHLPEHM
                     EDTANRLILPCH"
     gene            3140..3871
                     /locus_tag="ACHURS_003236"
     CDS             3140..3871
                     /locus_tag="ACHURS_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR1238892.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            3868..4929
                     /locus_tag="ACHURS_003237"
     CDS             3868..4929
                     /locus_tag="ACHURS_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1238893.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVTPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            5081..6127
                     /locus_tag="ACHURS_003238"
     CDS             5081..6127
                     /locus_tag="ACHURS_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFR1238894.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6124..6915)
                     /gene="nei"
                     /locus_tag="ACHURS_003239"
     CDS             complement(6124..6915)
                     /gene="nei"
                     /locus_tag="ACHURS_003239"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFR1238895.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGEPCERCGSIIEKTTLSSRPFYWCPGCQH"
     gene            complement(6951..7685)
                     /gene="pxpA"
                     /locus_tag="ACHURS_003240"
     CDS             complement(6951..7685)
                     /gene="pxpA"
                     /locus_tag="ACHURS_003240"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFR1238896.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQIMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQRGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(7675..8607)
                     /gene="pxpC"
                     /locus_tag="ACHURS_003241"
     CDS             complement(7675..8607)
                     /gene="pxpC"
                     /locus_tag="ACHURS_003241"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFR1238897.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTDGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLVWRLHNEN"
     gene            complement(8601..9257)
                     /gene="pxpB"
                     /locus_tag="ACHURS_003242"
     CDS             complement(8601..9257)
                     /gene="pxpB"
                     /locus_tag="ACHURS_003242"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFR1238898.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGFSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9280..10023)
                     /gene="ybgI"
                     /locus_tag="ACHURS_003243"
     CDS             complement(9280..10023)
                     /gene="ybgI"
                     /locus_tag="ACHURS_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFR1238899.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDV
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10294..11775
                     /gene="dtpD"
                     /locus_tag="ACHURS_003244"
     CDS             10294..11775
                     /gene="dtpD"
                     /locus_tag="ACHURS_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFR1238900.1"
                     /translation="MNKHASQPRTIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
BASE COUNT         2823 a         3161 c         2898 g         2939 t
ORIGIN      
        1 atattcttgc accgatatac ttttttcttt tctgttaaag gactcagtta tgtttaaagg
       61 acaaaaaaca ttggccgcac tggccgtatc tctgctgttc acagcacctg tttatgcggc
      121 tgatgaagga tccggtgaaa ttcactttaa aggtgaagtt attgaagcac cgtgtgaaat
      181 acatcaggat gatattgata aagaggttga actcggtcag gtgaccacca gccacattaa
      241 tcagtcacat cacagcgatg ccgttgctgt cgacctgcgc ttagtcaact gtgatctgga
      301 aaactccagc aacggttccg gtggcaagat ttctaaagtt gcagtcacct ttgatagctc
      361 agcgaaaacc accggcgcag atccgattct caacaacacc agcacgggtg aagccaccgg
      421 cgtcggcgta cgtttaatga ataaagatca aagcaatatc gttttaggca ccgctactcc
      481 agatatcgac ctggctccga cctccagcga acaaacgctg aatttctttg cctggatgga
      541 acaaattgat caggctacac ctgtaacgcc aggcgcagtt acggcgaatg caacctacgt
      601 gctggattat aaataagtat tactttggcg gcgctatcgc cgcctttcat aaaatattta
      661 tcaggtatgg acaccgtgaa tatttatcga ctctcttttg tatcctgcct ggtcgtggcg
      721 atgccttgcg cattggcggt cgaattcaac cttaatgttc tcgataaatc gatgcgcgac
      781 cgcattgata tttcattatt aaaggaaaaa ggagtcattg ctcccggtga gtattttgtt
      841 agcgttgcgg taaataataa ccaaatcagt aacgggcaaa agattaactg gcacaaaaat
      901 gacgataaaa ccattccgtg catcaatgat ttactggtcg ataaatttgg cttaaaacct
      961 gaagtccgtc agtcgttacc attgataaat cagtgcgtcg attttagctc ccgacctgaa
     1021 atgctcttca atttcgatca agccaatcag caactgaata ttagtattcc gcaagcctgg
     1081 ctggcgtggc actcagaaaa ctggccccca ccctccacat ggaaagaagg tgtcgccggt
     1141 atcctgatgg attacaactt gtttgccagc agctaccgcc cacaggacgg cagcagcagc
     1201 actaacctga acgcctacgg taccaccgga attaacgccg gggcatggcg cttacgtagt
     1261 gattatcagt tgaatcagac tgatagcgat gataaccatg aacagtcagg cggaatatcg
     1321 cgcacctatc tttttcgtcc attaccgcaa ttaggctcta aattaaccct cggcgaaacg
     1381 gattttagtt ccaatatttt cgacggtttt tcttataccg gcgcggcact ggcaagtgac
     1441 gagcgaatgt tgccatggga actacgcggc tacgccccac aaattagcgg tattgcacag
     1501 accaatgcca cggtgacgat cagtcaatca ggccgcgtca tttaccagaa aaaagtccca
     1561 ccaggcccat ttatcattga cgaccttaat cagtctgttc agggcacact ggatgtcaaa
     1621 gtgacggaag aagatggtcg ggtgaacaat ttccaggttt cggcagcatc gacgcccttc
     1681 ctgactcgtc agggacaggt tcgctataaa ctggccgcgg gtcagccacg gccctccatg
     1741 tcacatcaaa ctgaaaatga aacctttttt agcaatgaag tttcctgggg gatgctgtca
     1801 aacacctcgc tgtacggcgg cctgctgctt tctggtgatg actaccattc tgccgcaatg
     1861 ggtattgggc aaaatatgct gtggcttggt gcgctgtcgt ttgatgtcac gtgggccagt
     1921 agccattttg atactcagca ggacgagcgg ggcttaagct accgttttaa ttacagcaaa
     1981 caagtggatg ccactaacag cacgatttcc ctcgccgctt atcgtttctc cgatcgtcat
     2041 tttcacagct acgccaacta tctggatcac aaatacaacg acagcgatgc gcaggacgaa
     2101 aaacagacga tcagcttatt gtgggccaac cgattacccc actaaacctc aatctttacg
     2161 ccaacctgct acatcaaacc tggtggaatg cagacgcctc cacgaccgcc aacatcacgg
     2221 caggttttaa tgttgatatt ggtgactgga gagatatctc tatttcgacg tcattcaata
     2281 caacccatta cgaagataaa gatcacgaca accagattta cctgtcgatt tcgctcccct
     2341 tcggtaacgg tggtcgggtt ggctatgaca tgcaaaacag tagccacagc accacacacc
     2401 gcatgtcgtg gaacgatacg ctggatgaac gtaatagctg gggcatgtct gccggactgc
     2461 aatccgaccg tcctgacaat ggagcccagg tgagcggtaa ctatcagcac ctgagttcag
     2521 cgggtgagtg ggatatttct ggtacctatg ccgccaatga ttacagttcc gtcagcagca
     2581 gctggagcgg ttctttcacc gcaacccaat atggtgcagc gtttcatcgc cgcagctcca
     2641 ccaatgaacc acgcctgatg gtcagcaccg atggcgtggc agatattccg gttcagggca
     2701 atctcgacta caccaaccat tttggcattg cggtggtgcc gttgatttcc agttaccagc
     2761 cttccaccgt ggcggtgaac atgaatgact tacccgacgg cgtaacagtt gcagaaaacg
     2821 ttatcaaaga aacgtggatt gaaggcgcga taggttacaa atcactggct tcccgttccg
     2881 gtaaagacgt taacgtcatc attcgcaacg ccagcggtca gtttcctccc ctcggagcgg
     2941 atatccgcca ggatgacagc ggcattagcg tggggatggt tggcgaggaa ggacatgcct
     3001 ggttaagcgg agtcgctgaa aatcaaaagt ttaccgtggt ctggggtgat agccagcatt
     3061 gctcgctcca tcttcctgaa catatggaag acaccgcaaa tcgcctgatt ttaccttgtc
     3121 attaattaag gaaatgaaaa tgacatttat gaaaggacta ccccttttgt tgttggttgc
     3181 cagtctgtgc agccacgctg cactacaacc cgatcgcact cgtattgtgt ttaacgctaa
     3241 tgataaagct accagtctgc gggtggataa tcgtagcgat aaactcccct acctcgctta
     3301 ttcctggctc gaaaatgaaa aaggggagaa aagcgatgat cttctggtcg ccctgccgcc
     3361 gatacaacgt ctggaaccga aagcaacaac gcaggtacgg atagtaaagc aagcctcgac
     3421 cacaaaatta ccgggcgatc gcgaaacgct atttttctac aatatgcgtg aaattcctcc
     3481 cgccccggaa aaaaacagcg accatgccgt tctgcaagtc gccattcaga gtcgtatcaa
     3541 ggtgttctgg cgaccggctg cattacgcaa gaaagcagga gaaaaggtcg aactgcagtt
     3601 acaggtcagc cagcagggca accaactgac actgaaaaat cctaccgcgt attatctgac
     3661 cattgcctac ctgggacgaa atgaaaaagg cgttctcccc ggcttcaaaa ccgtgatggt
     3721 tgcgcctttt agtacggtca acacaaacac cggaaattat agcggcagcc agttttatct
     3781 cggttatatg gatgattacg gcgcgttacg catgacgacg ctcaactgca gcggacaatg
     3841 ccatttacaa gccgtggagg cgaagaaatg agtgctggca agggagtgtt gctcgtcatc
     3901 tgtctgttat ttctgccgtt gaagtctgcg cttgccctga actgctactt tggttcatcc
     3961 ggtggctcgg tagaaaaatc agaagcgatt caaccttttg ctgtaccagg caatgccaaa
     4021 ctcggcgata agatttggga atctgacgat attaaaattc ccgtctattg tgacaacaat
     4081 accaacggca attttgaaag tgagcatgtt tatgcctggg taacccccta cccgggggtg
     4141 caggatcgct attatcaact cggcgtaact tacaatggcg ttgattatga cgccaaccag
     4201 ggaaaaagcc gcatcgacac taaccagtgt attgacagta agaacattga catttatacc
     4261 cctgagcaga ttatcgcgat gggatggcag aacaaaattt gctcgggtga tcccgctaat
     4321 attcacatgt cgcgtacctt tctcgctcgc atgcggttat acgtcaaaat acgagaaatg
     4381 ccgccgcatg attatcaaag tacgcttagc gactatatcg tcgtgcaatt tgacggtgcc
     4441 ggtagcgtta acgaagaccc tactgcccaa aacctgaaat atcatattac tggtctggaa
     4501 aacattcgcg tgctggattg cagcgtcaat ttttccattt caccggaaac acaggtgatt
     4561 gattttggta aatttaattt gctggatatt cgtcgccaca caatgtcgaa aacattcagt
     4621 attaagacga caaaatcaca aaatgaccaa tgcaccgacg gatttaaagt cagttcctcc
     4681 ttttataccg aagaaacgtt ggttgaagaa gataaagcat tgttgattgg taatggtctg
     4741 aagctacgtt tgctggatga aaacgcctcg ccttatacct tcaataaata tgctgaatac
     4801 gccgatttca ccagcgacat gttggtctac gaaaaaacct atacagctga actttcgtcc
     4861 attgcaggta cccccatcga ggctgggccc ttcgatacag tggtgctttt taaaattaac
     4921 tataactgat ttgccatgat gccctttgca gccggaaagg gcatctcttt cagaaaattg
     4981 ctgattttcc ccttttcctt gattgctgtt attttttcct ggcccggctt tctttatgct
     5041 gtatcgatac accaaagaga aaataatgag ggagcgaagg atgccagttt tgcagtgggg
     5101 aatgttatgt gtgctatcac ttctcctttc tattggtttc ctcgcggtac atctcccggc
     5161 ggcgctatta cttgggccga tgatcgccgg gatcatcttc agtatgcgcg gaattactct
     5221 gcaacttccc cgctccgctt ttctcgccgc gcaggccatt cttggctgca tgattgcgca
     5281 aaacctcact ggttcgattc tcaccacgct ggccgccaac tggccgatcg tgcttgctat
     5341 tttactggtg acgctgcttt ccagcgccat cgtgggttgg ttattggtgc gctatagctc
     5401 actgcctgga aataccggtg cctggggttc ttctccaggc ggcgcagcgg caatggtcgc
     5461 tatggcgcaa gattacggcg cagatattcg cctggttgca tttatgcaat atctgcgagt
     5521 gctgttcgtc gcgggggccg ctgttctggt cacccgcatg atgctgggtg ataacgctga
     5581 agcggttaac cagcaaatcg tctggttccc gccagtgagc attaatctcc tgcttacgat
     5641 tttgctcgca gtcgttgccg gtacggcggg atgcatgctg cgtcttcctt ccggcacgat
     5701 gctcatcccg atgctggcgg gcgcagtgct ccagtctggt cagctcatca ccatcgaact
     5761 accggaatgg ctgctggcga tggcgtatat ggcaattggc tggcggattg gtcttggttt
     5821 cgataaacaa atcttactgc gggcactacg cccgctaccg caaatcctgc tgtcgatttt
     5881 tgctctgctg gctatttgtg cgggtatggc gtgggggctg acccggttta tgcacattga
     5941 ttttatgact gcctacctcg ccaccagtcc cggcgggctt gataccgtgg cggtcatcgc
     6001 cgcagggagc aatgccgata tggcgcttat tatggcgatg caaaccctgc gcctgttcag
     6061 tattttgctg acggggccag ccattgcgcg gtttatttca acctatgcgc cgaagcggtc
     6121 ggcctagtgc tggcagccag ggcaccagta aaacgggcga gatgacagcg tggttttctc
     6181 aatgatgctg ccacagcgtt cgcacggttc gccatcacga tgaaaaacct taaagcgaaa
     6241 cagcgcccca tgatgcttat tctcatccac ctgccccctc gtagcgtagg aaaatcgagg
     6301 aatatccagt aacgcgtgtg cgagtgcatc cagttgcgcc gcattgagat ctttcgcttt
     6361 atgatttcca gtcaacccaa cctgccagag gatctccacc cgcaaataat tgccaagccc
     6421 agccagaaac gcctgatcga gcagtaatcc tgcaaactga cggttacgaa agcgcggtga
     6481 caataaccgt tcctttacca cctccggcgt cagattcgga tccagcacat cgggaccaac
     6541 gcgttgtaaa aacggatgcg tggtcagttg ttccggcctc aacatctcaa tatccgaggc
     6601 gctataaagc agaatggttt tgtcagccgt ttgcagtttt acccgcaata ctcgcgtggt
     6661 ctgcggctct tcgccggtat caaccacgcg ccagacgccg taaagctgat tatggctgta
     6721 gagcgttaag tcgttggaaa aatgagttaa caacgcctta ccacgcgttt ccacatgggt
     6781 aacgtgttga ccgataagtt gtgattgata agtttttaac tgcgggaagg caaaccagac
     6841 atcagttagt ggtttgcctt tgatcgccgc ctccaggtta tccgctgcac ggcggatctc
     6901 cgggccttca ggcatggtga tatccttttt tcattcttta ttaatcgggg ttatgctgcg
     6961 acaacaatct ccttttcggc aaatgtagag cgtagtcggc gggcgaacgc cagtgcgtgc
     7021 tcgccgtcgc catgcaggca gactgtttgc gccgtgaccg ttgcccattc gccggtgata
     7081 cttttgactc tgccgtgttg caccatctct agcgtttgcg ccaacgcctg ttcttcgttt
     7141 tcaatcaacg cgcctcgctg acttcgcggc accagcgagc cgtcagcctg ataaccgcga
     7201 tcggcaaaca cttcctcgcg cgttgtcagg ccatattgct tgcctgcacg aatcagctcg
     7261 cttcctgcca gcccaacgag aatcaatgcc ggatcgcaag cgtataccgc tctggcgatg
     7321 gcgtctgcca gttgtgcttc tttcgccgcc tggttgtaca acatgccgtg cggtttgaca
     7381 tgacgcatta cgccgccttg cgcacgggca atcgttgcca gcgcgccaat ttgatacagc
     7441 gtctgggcgt aaacggtttc tggcggcagc tgcatggcgc tgcgaccaaa attttccctg
     7501 tcgggaaaac tcgggtgagc gccaatcgcg acaccatttt ttattgcttc acgcacgcaa
     7561 gcctgcatga tttgcgcatc gcctgcatga aatccacagg caatattggc agaggaaacc
     7621 agcgttaata gctctgcgtc gctggcgcag ccttcgccca gatcggcgtt caggtcaatt
     7681 ttcattgtgc agccgccaca ctaattgttc gaaataacgt tgctgatctt gccgcgcttt
     7741 cagtgcctct tccagtgaac actggacaaa atgaatcggc tgaccgagcg gaatttgcgc
     7801 cagatggtac atatcagcct caatgataca ggcaatacgc gggtaaccat cggtggtctg
     7861 tgcgtcgttc atcaacacaa tcggctgccc gttatgtggc acctgcacca cgcccggtaa
     7921 caaaccgtga gataacagtt cgcgatcggt ggtgcgtttt aaaatttgcc cctgtaagcg
     7981 atagcccatg cggttacttt gcgagctaag ctgccagggc gaacgccaga atgcatcctg
     8041 cgaggcgcga tcgaactcat gatattccgg ccccggcaag gcgcgaatgc ggttgcccca
     8101 cagcagctgt ttaacgccct gcgcttccat aaaatcacgc ttcgatttgc caatcgggag
     8161 tcggtcacca tccttcagta aacggccttc cagcccgcca atccccactt tgagatcggt
     8221 gctgcatgac cccattaccg gcggaacatc aataccaccc gcgaccgcca gataactgcg
     8281 catcccgtgc tgcgggcgtt taagcgttaa acgctggcct gctttcatcg gcaatcgcca
     8341 gccggtccag acggcattat catccagccg cgcttcgcaa ccggcacccg tcagagcaaa
     8401 ccacccatca gtttcgaact caaccgttaa ctgaccgagc gtgatctcca gcgcgggggc
     8461 attggcgtca ttacccacca gcaggttagc aatgcgtaac gcaggcatat ccagtgcgcc
     8521 gcagtggctg attcccgact ggcgaaaacc gtgacgaccg ccatcctgca cagtggtata
     8581 catgcccgca cgaataatct tcagcatact ccctccttct gcggtacaaa gcgcacgctg
     8641 tctcccggac gtaataagat gggttcgtca cgcgccggat caaacaggct gagtgaggta
     8701 tgaccaatca actgccagcc acccggcgtt gccagcggat aaacaccagt ctgcggcccg
     8761 ccgatcccga cagaacctgc cggaacgagc aagcgcggtt cagcgcgccg tggcgtgtgt
     8821 agttgttccg gcaaactccc gagatacggg aagcccggtt gaaaacctaa aaaccagacc
     8881 acgtattcca cggaggagtg caattcaaca acctgttttt cgctaaaccc gcaatgcgcc
     8941 gcgaccaccg ccaaatccgg tccgcctgca ccaccgtaaa ccaccggaat ttcaataaag
     9001 cgagactcag gttccagggc ctcgctctcc tcccaccagc gttgcaaacg ctctatggca
     9061 tccagcgcca gcgactcagg attacgcaaa atcaccgtga tattgttcat gccgggaatg
     9121 gcttcaacca cattcggcat atccaccaga cgctgcgcca gtcgccagat ccgtttctgg
     9181 ctagccagcg tcaccggcgg ttccagttcc agcactaccg ccgtttcacc tatcagataa
     9241 caacgcgctc gttgcacttt cgatccctct gattattcgt tatgcaggat taggaatatc
     9301 aataaaggtc acatcaagat cggtattttc attcagccac tcgctcaatg cgcgaatacc
     9361 accacgttca gtggcatggt gacctgcagc ataaaaatgc aacccctgct cgcgggctga
     9421 atgaatggtc tgttcagaaa cttcgccagt aataaaagca tccacgccaa aacgcgcggc
     9481 gctatcgata aaactttgcc cgccgcccgt gcaccaggcg acgcgctgga ccacctcagg
     9541 tccggtatcg ccacaccata acggcttacg tcccagacgc gcttcaatcc aggaggctag
     9601 ttccagtccc ggcacaggca tggtcagctc tccccacggc accaacggct caatttcgcc
     9661 catgaccgtg atccccagta acgccgccag ctgtgcgtta ttgcccagct caggatgtac
     9721 gtcaagcggc aaatgccagc catacaggtt gatatcattc gccagcaacg tttttaaacg
     9781 gttacgcttc attccgcgaa tgaccggaga ctctcctttc cagaagtagc catgatgcac
     9841 aatgaccgca tccgcgccca gacgcactgc ctcatcgagc aaagcctggc tggcggtgac
     9901 accggtaaca attttttgca ccgtctcttt gccttctacc tgcaaaccat tcggcgcgta
     9961 atcactaatc gccgcgctgt tcagtttttc gttaatcagt tgttccagtt cggtgttttt
    10021 catcttcgcg ccttccgtat tctgatagct acaccgttaa catagcgtta cctcgcctgt
    10081 tcgtcgatag ccaatctttt gacatattaa gaaacaaacc cgacgccaaa agacgcaaat
    10141 tgtttatatc atcacgcttc agctaatgta tatttatgca atcagacagc atattgcata
    10201 aataatcaga tctgtatcaa agttttaact ttaaggaaac acaagcacgc cgcgcaacta
    10261 ttacgtgacg ttgcagtcaa ttgtggaatt attatgaata aacacgcatc acaaccgcgc
    10321 actatttact atgtcgttgc gctgcaaatc tgggaatatt ttagctttta cggcatgcgt
    10381 gccctgctga ttctctatct caccaatcaa ctaaaataca acgatactca cgcctacgag
    10441 ttatttagcg cctactgttc gctggtgtat gtcacgccaa tcctcggtgg ctttttggcg
    10501 gataaagttc tcggcaatcg catggcggtg atgctggggg cgttgttgat ggcgatcggt
    10561 catgtggtgc tgggtgccag tgagatccat ccgtcattcc tctatctgtc cctggcgatt
    10621 atcgtttgtg gctatggtct gtttaaatcc aatgtcagtt gcctgctcgg cgagctgtat
    10681 gagccaaccg atccgcgtcg tgatggcggt ttctcgctga tgtatgcagc gggtaacgtg
    10741 gggtctatta tcgcacctat cgcctgtggt tacgcccagg aagagtacag ctgggcgatg
    10801 ggctttggcc tggcggcggt gggtatgatc gcgggtctgg tcattttctt atgtggcaat
    10861 cgtcatttca cacatacccg cggcgttaat aaaaaagtac tgcgtgcaac aaactttctc
    10921 ctgcctaact ggggatggct gctggttctg ctggtggcaa cgcctgcgct gattaccgta
    10981 ctgttctgga aagagtggtc ggtatacgcc ttaattgtcg cgactattat tggcctgggt
    11041 gtactggcaa aaatttatcg caaagcagaa aaccagaaac agcgtaagga gctggggctg
    11101 attgtgacgc tcaccttctt cagtatgttg ttctgggcct tcgcacaaca gggcggcagc
    11161 tcaattagcc tttatatcga ccgcttcgtt aaccgcgata tgtttggtta taccgttccg
    11221 actgctatgt tccagtcgat taatgccttc gcagttatgc tatgcggtgt gttcctggcg
    11281 tgggtggtta aagaaagcgt cgcaggtaat cgtaccgtgc gcatctgggg aaaatttgct
    11341 cttggccttg gcctgatgag cgccggattc tgcattctga ccttaagcgc ccgctggtcc
    11401 gcaatgtatg gtcactcttc tctgccactg atggtgttag gcctcgcggt gatgggcttt
    11461 gcggaactgt ttatcgaccc ggttgccatg tcgcaaatta cgcgcattga aattccgggc
    11521 gtgaccggcg tattaaccgg catctacatg ctgctttctg gcgcgatcgc gaactatctg
    11581 gcgggcgtga ttgccgatca gacatcgcag gcttcgtttg atgcttccgg ggcgatcaac
    11641 tactccatca atgcatatat tgaagtattt gatcaaatta cctggggcgc actggcgtgt
    11701 gtaggactgg tactgatgat ttggctgtat caggcgctga aattcagaaa ccgcgcgctg
    11761 gcgctggagt cataataggc cagcaccgat gcctgatgcg acgcttgacg cgtcttatca
    11821 g
//
LOCUS       DAZPDU010000134        11779 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZPDU010000134 DAZPDU010000000
VERSION     DAZPDU010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11779
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.135"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..611)
                     /locus_tag="ACHURS_003245"
     CDS             complement(<1..611)
                     /locus_tag="ACHURS_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFR1238901.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRR"
     gene            complement(734..2380)
                     /gene="fumB"
                     /locus_tag="ACHURS_003246"
     CDS             complement(734..2380)
                     /gene="fumB"
                     /locus_tag="ACHURS_003246"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFR1238902.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(2458..3798)
                     /gene="dcuB"
                     /locus_tag="ACHURS_003247"
     CDS             complement(2458..3798)
                     /gene="dcuB"
                     /locus_tag="ACHURS_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFR1238903.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(4369..5088)
                     /gene="dcuR"
                     /locus_tag="ACHURS_003248"
     CDS             complement(4369..5088)
                     /gene="dcuR"
                     /locus_tag="ACHURS_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFR1238904.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTDWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(5085..6716)
                     /locus_tag="ACHURS_003249"
     CDS             complement(5085..6716)
                     /locus_tag="ACHURS_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFR1238905.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLANSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            6897..7127
                     /gene="yjdI"
                     /locus_tag="ACHURS_003250"
     CDS             6897..7127
                     /gene="yjdI"
                     /locus_tag="ACHURS_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFR1238906.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            7139..7411
                     /locus_tag="ACHURS_003251"
     CDS             7139..7411
                     /locus_tag="ACHURS_003251"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1238907.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            7638..7934
                     /gene="ghoS"
                     /locus_tag="ACHURS_003252"
     CDS             7638..7934
                     /gene="ghoS"
                     /locus_tag="ACHURS_003252"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFR1238908.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            7962..8135
                     /gene="ghoT"
                     /locus_tag="ACHURS_003253"
     CDS             7962..8135
                     /gene="ghoT"
                     /locus_tag="ACHURS_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFR1238909.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(8254..9771)
                     /gene="lysS"
                     /locus_tag="ACHURS_003254"
     CDS             complement(8254..9771)
                     /gene="lysS"
                     /locus_tag="ACHURS_003254"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFR1238910.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKNNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIITEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(10008..11465)
                     /gene="dtpC"
                     /locus_tag="ACHURS_003255"
     CDS             complement(10008..11465)
                     /gene="dtpC"
                     /locus_tag="ACHURS_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFR1238911.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMVIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(11524..>11779)
                     /locus_tag="ACHURS_003256"
     CDS             complement(11524..>11779)
                     /locus_tag="ACHURS_003256"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="constitutive; catalyzes the formation of cadaverine
                     from lysine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFR1238912.1"
                     /translation="TEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEF
                     LQMLCEIGAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
BASE COUNT         3044 a         2858 c         2892 g         2985 t
ORIGIN      
        1 aagcgccgta gctggcggca atgaaagcga gcgaggaata tcaccgtcgt taatgcgcca
       61 agcagcgcgc agaacatatc ggattgcgtg tcccactggt cgccctgggt gccgagaaaa
      121 tcatccgctc cctggcccat tgccagcgcc gcccaccact cgattaattc atacatcgcg
      181 cttatcgcca gcgccacgca gcagactagg aacgccacca ttttacgtcc gcgcacgtac
      241 atcccgcgca cgagaatttc tcgtgccacc agtgcaggca ccagcccctg gaaaaaatgc
      301 cccagcttgt catacggatt acggctcaac cccagccatt cctgcacctc aaaaccgatg
      361 gggactttcg cgtaggtgta ttgcccgccg accatcagga tgatggcgtg aaagaaaatg
      421 agcgtataga gcagcggcgt taacggataa cgtttagcgg tggcaagcag tagcggcacg
      481 acaataatca ccggtgtcac ttccatcagc caggtgagtt tgtcatgggc cgaaatgccg
      541 gtataaatca ggattaacgt tagcgccagt gcgccggtgt taagaattaa cggtttgagt
      601 gtgcgggtca tggtgagttc gcaagtcagg gaaaatactg actattcacc agcaagcatg
      661 aaattacaat cgcaaaagag gtggcccagg gggatcacct ggcagcatgc tgccaggcgc
      721 tgggccgaag aggttactta gtgcagttcg cgcactgttt gttgacgatt tgctggaaga
      781 agtcgttacc tttgtcatcg accaggataa acgccgggaa atcttctact tcgattttcc
      841 agatagcttc catacccagc tccggataag cgacgcattc cagatgcttg atgctctgct
      901 gcgccagtac cgccgccgga ccgccgatgc taccgaggta gaagccgccg tgtttatgac
      961 acgcgtcggt aacctgctgg ctgcggttgc ctttcgccag catgatcatg ctgccgccgt
     1021 gggattgcag cagatccaca taggagtcca tacgacctgc ggtggttggg ccaagtgaac
     1081 ctgatggata accggcaggg gttttcgccg gacccgcgta gtagatcggg tgatctttga
     1141 tgtactgcgg aagttcttta ccggcgtcaa tcagctcttt cagcttggcg tgtgcaatat
     1201 ctcgacccac aataatggtg ccagtgagcg acaaacgagt ggaaaccgga tattgcgaaa
     1261 gctgggcgag gatctctttc atcgggcggt taaggtcgac cttcaccgct tcgccttcac
     1321 cggcctgacg cagttcttgt ggaatgtact gacctggatt gtgttccagt ttttcgatcc
     1381 agataccttc gcggttgatt ttcgctttaa tgttacggtc agcggagcag gagacgccca
     1441 tgcctaccgg gcaggatgcg ccgtgacgtg gcagacggat aacgcgaatg tcgtgcgcga
     1501 agtatttacc gccaaactgc gcgccaagac cgagtttctg ggcctcttcc agcagttcct
     1561 gttccagctg gacatcgcgg aacgcctgac catgttcgtt cccttccgtc ggcagttcat
     1621 cgtaatagtg agcgcttgct aatttgacgg ttttcaggtt ggtttccgca gacgtaccgc
     1681 caatcacaaa cgcgatatgg tacggcgggc aggctgcagt accgagggta cgcattttct
     1741 cgacgaggaa gtttttcagt ttgccgggag tcagcagggc tttggtttcc tggtagagat
     1801 acgttttgtt ggcagagccg ccgcctttcg caacgcaaag gaatttgtac tcatcgccat
     1861 ctaccgcgta caggtcgatt tgcgcaggca ggttagtgcc ggtgttgacc tctttgtaca
     1921 tgtccagcgc cgcgttttgc gagtagcgca ggttatcttc gatataggtg ttatagacgc
     1981 ctttcgacag cgcttcttca tcaccgccac cggtccacac gcgctggcct tttttaccga
     2041 cgatgatcgc ggtgccggta tcctggcagg tcggcagcac gcctttggcg gcgatttcgg
     2101 agtttcttaa gaattgcagt gccacgtact tgtcgttttc gctggcttct ggatcgtgaa
     2161 gaatagccgc aacctgtttc tggtgtgccg gacggagcat gaaagaagcg tcgtgaaagg
     2221 cttgctgcgc cagcagggtc agggcttctg gttccacttt caggatggtt tcgccgtcga
     2281 agtcggcaac gctaacgtaa tcggaagtga gtagatagta ttcggtattg tctttcccca
     2341 tcgggaaagg tgcctggtag ataaagggtt tgtttgacat agcttccagc ctgtaactct
     2401 gtatttgtta ttcgaaaatg gcgtgccgcg tagtcacggg cggcacgcaa agtgcattta
     2461 taagaacccg tacatcgcgg cgaagatcca gccgaagacg cacgatacgc tcacaccaat
     2521 caaccccggc agaataaagc tgtggttgat gacgaagcga ccgatgtggg tggtgccgga
     2581 acggtcaaac tgaatcgctg ccagatcgct cggataagtc ggcaggatgt aataaccgta
     2641 gcaagccggt gctgaagcca cgatgtatgc cggatcaacg ccgatcgcca gcgcgaccgg
     2701 aacaatcgcc gccagcgccg cagcctgaga gtttacaaac ttggaaacca gcagcagaac
     2761 aatggcatag gcccacggat actctttcac catttcaccc agtacgccct gaatttcaga
     2821 catatgcgca ccgaacatgg tttctgccat ccatgcgata ccgtacaccg ccacgatggc
     2881 gatcataccg gaacggaaga cttcgttttt tgagatagac gcgggattgg ttttggtcag
     2941 gataataatc agcgccccgg tcagcagcat aaacatctga ataaccagta ccatcgacag
     3001 cggtttgccg ccgaaggatg gacgcaggtc cgaatcagca ccaagaaggg cgactacagc
     3061 gattgccccg aggaaaatcc acattgccag ccagttgctt ttcggcagtt ttttgtccag
     3121 cagcgtcgcg gtatcaccgt aaacatactc acggttttcc ggtacggaga tgaatttctg
     3181 gaactcttcg tctttatcca gatctttacc acggaaccag ctgaagatac cgatcgccag
     3241 gataccgatt aacgtcgatg gaatggtgat tgccagcagg tcgaggaact caagatggcg
     3301 accatcaaag gtgacattac ccagcattgc aaccagagac acgaccgcaa ccgacaccgg
     3361 actggcgatg atccccatct gtgcaccgat agaacttgcc gccatcggac gttccggacg
     3421 gatgttgttc ttaatggcga cgtcgtagat gatcggcaga atggtgtaaa ccacatgacc
     3481 cgtaccgcaa agaatggtca gcgtacaggt cacaaacggc gcgacaattg agacatattt
     3541 cgggttgcgg cgcagcagct tctcggcaat ttgcagcatg acatcaagac cgcccgaagc
     3601 ttgcaaggtc gccgatgccg ccaccaccgc aatgataacc agcatgacat caaccggtgg
     3661 tttacctggc tgaaggtgga agacgaagac cagaatgacc agaccgatac cgcctaataa
     3721 acccagcgcg ataccaccct ttctggcacc ataaaacaga catatcagta ttatgataag
     3781 ttggatagta aataacatgt gtgaaccctc gcgataatcc tatttaaatt tttgctgaat
     3841 agatcacagt cacgttctgt tttgtatgaa ctgttttcag agcctgacta aatatccgtt
     3901 tggtcgttac cggctttagc aaatacctca cagtgaatat tggctgatta aagcagcggc
     3961 aaatttatgc actgtgataa gcggcttttt cagagagaaa gactctctgg cgatgggttt
     4021 atagtttgta ctgcgcagat gttaatgttt tgtttgctgt tatcttattg atatttatcg
     4081 gtctattttt tgttctcagg gcagatgtca ttaggtttat agattaatct gatctaccca
     4141 tttgtgggta aaaatacaca taatgcgggt gacataatag ttaattaact tttgttagcg
     4201 ttttgaaatt aaaaacaccg ttcacctgaa gagatattaa tttttagcga tgatgcaggg
     4261 ataattatat ttgatctggc acaagtttta ctgatgaagg atgtaacttg tgccaggggt
     4321 attttgcatt acggtaataa ttattactta cagataacca gcgatccgtt attggcaata
     4381 ttgtttcagt agtgagtagt gttctgcctg aatacggtaa cggtaaactg gacgccccgt
     4441 gacgccataa tggatactgg tgaacaagat gtggcagttg accagccaga tgaggtattt
     4501 acggcaggaa acacgcgaaa tgttaacctc gttggctagc tcgtcggttg aaaattcata
     4561 gtcctgatgc gcgtcaatcc actggcacag tgtgcgtaac gtctgcggcg ttaagccttt
     4621 tggcaagcga cgaggatcct gttcgttgga gctgctgccg tggattagct gatcaagctc
     4681 ggcctggtca taatactgat gtttttccag cgccattttc ttttgccgcc agtcggtgag
     4741 cgcctcttca aagcgggaag cctggaaggg tttgatcagg taatccacga caccgtaatg
     4801 cagcgaatct ttaatggttg ccgcatcggc tgcggaggag atgacaatca catcactttt
     4861 gcaacgcgcg ttatgcagga caggcagtaa atcgagcccg ttctcttttt gcatatagat
     4921 atcgagcaat atcaggtcga taggcgtatc gctattgaag ataatctctt tggctttctc
     4981 cagcgtcgag gctgttccac agcattgaaa gcctgggatt tgtgctacgt atcggcgatt
     5041 cagctccgcg accattgcgt cgtcatcgat aattaataca ttgatcatct gttcgacctc
     5101 tccccgtccc agggtatctg gacaaaaaat tgtgtgaaaa tcccgggttc cgattccacg
     5161 gcgatgctgc cgccgagatt ttctacctgt tgtttgacaa gtgctaaacc gacgcctcgc
     5221 tcgcttcctt ttgtcgagac acctttgtca aaaatgtgat cgattttgtc gggtgcgatc
     5281 cccggtccat catcgttaac ttcgcagtgc agccagccgt gacggtagtg caatgttacg
     5341 ctaatttcgc caccgggttc cggccctaat gcttccagcg cgttttctat cagatttccc
     5401 aatgtggtaa tcagcgtcgc gacctggtcc tcactgccgc tgtctggcag ctggctttca
     5461 ctgtttaaaa tcagcgtatg gcctaaatcg gtcgcgcgat taatcttgct gattaaaaaa
     5521 ccagcgataa ccggagattt gatcttaccc agcagagagc caatctcttc ctgatagtta
     5581 ttggctgttt ttagaatgta atcttccaac tgcttataac tcttcagatg caataatccg
     5641 agaatcacgt gcaatttatt cataaattcg tgggatcgtt cacgaagtgc gtcggcatag
     5701 ttgaccagac cgtcgagtcg ctgcatcagt ttacgtactt cggttttgtc cctgaaggtt
     5761 gaaatggcac cgatgataac gccattactg cgcaccggaa cggtgttgat cagtaatagc
     5821 cggtctttaa tcgtaatctc ttcgtcgcgg cgcggcgtac cgtcgcgtaa cacttccgag
     5881 acatctacca cctgtgacca tgagtggctg agcgtcgaca gtttctcatc gtcctgcgac
     5941 ttacggtaat tcagcaattc ttgtgcggca tcgttgatca gcgtgacctc gccgcgatcg
     6001 tccacggcaa cgacgccttc tttgatagac tgcaacatgg cctggcgttg ctcaaacagc
     6061 gtggagattt cgtagggttc caggccgaaa aggatttttt tcagtacctt aaccagaatg
     6121 caggtgccaa tcagtccgac cagcatgcca aataataccg accagataat gctccagcga
     6181 ctgtcattga tttgttgggt cacgcggctt aactcaaggc cgatcgccac cacgccaatt
     6241 tgtttatgat tttcatcgta gatgggggta aatacgcgta aagcctgcgc cagaaaaccg
     6301 cgattgatag cgacattttc tttgccattc agcgctttaa ggatgtcatc acctttaaat
     6361 ggctgaccaa tacgctgggc ttcaggatgc gagtagcgaa gactttgcat atcggtaacg
     6421 acaataaaca gcagatcgtt gcgtttgcgc acggcttccg cgatggcctg gatgccgctc
     6481 tcctgcggtt ttttctgcaa gccctgacgg atttccggcg agttggcgag ggtacgcgcc
     6541 actgccagtg ccttgttggc tagcccgtct cgcgtcatat cactgatttg cgagaagtaa
     6601 atcagatgca ccaccaatag caccgagaac agtaccgcac tgaccattaa gatcactgtg
     6661 gtactcaatt tcatcggacg tttgcgtaac atgcggtagg gcaatgaatg tctcatcagc
     6721 ttccttgtgt gacaaatttc ttaaacatta tctctgatga ggcgggtaat tcaaagggag
     6781 taagaatgat tggctatata ggggaagaga ttctggcaac ggaaactgcc agtgctgtat
     6841 gaagattcca gggctatgct tatagcgata atcatactga tgagagaggg aaagtcatgg
     6901 atcaggcgct actggacgag ggttatcgct gttataccgg cgaaaagatc gatgtctatt
     6961 tcaacactgc gatatgtcag cattctggca attgcgtacg tggcaacggc aagttattta
     7021 atctcaaacg aaagccgtgg atcatgccgg atgaagtcga cgtcgccact gtggttaaag
     7081 tgattgatac gtgcccgagc ggcgcgctga aataccgtca taaataagcg agggtaaaat
     7141 ggaaatacgc gaaggccaca ataaatttta cattaatgac gaacaaggca agcaaatcgc
     7201 tgaaattgtc tttgtgccga ctggagagaa tttagcgatt atcgaacata ccgatgtcga
     7261 tgaaagcctg aaagggcagg ggattggtaa acagctggtt gcaaaagtcg tggaaaaaat
     7321 gcgtcgggaa aaacgaaaaa ttatcccact atgcccattt gcgaaacacg aatttgataa
     7381 aacgcgggag tatgatgata ttcgcagttg atgggagagt acagagtcac gatatttttc
     7441 atgctctccg cggagtgatg caggagagca tctgaagggt agggggatgc acaaagaatg
     7501 ggcagagagc gcgttttttt gtcccaagtc atccccttta ctgagcaaaa aaaagaatat
     7561 ctcctatatg agaatcatca atcggggtta tcaagttttg cgtccccaga gcattcaata
     7621 ttgataggag tcatattatg gaaggtaaaa acaagttcaa tacttatgtt gtttcttttg
     7681 attatccatc atcttattcc tcagtgttct taaggttaag atcattgatg tatgatatga
     7741 atttctcttc tatcgtggct gatgaatatg ggataccacg acaattgaat gaaaattcct
     7801 tcgcaataac gacatcgtta gccgcaagtg aaatcgaaga tttaatcagg cttaaatgct
     7861 tagacttacc ggatattgat tttgacctca acattatgac agttgatgac tatttccgtc
     7921 agttttacaa gtagccagga aggataagaa gaacataaaa aatggcacta ttctctaaaa
     7981 tattaatttt ttatgtgatt ggtgtgaaca tatcctttgt cattatctgg tttatctcac
     8041 atgagaaaac acatattcgt ttacttagtg cattcctggt cggaataacc tggccaatga
     8101 gtctgcctgt ggcattactt ttttctctct tttaggagct gtagttgagt ggacccgctg
     8161 gttatcgtga aacgaagcgt ctctcctcaa aactggattt gcggaacgac gaacgaagca
     8221 gtcgcctgac tgcttcgttc attaaagtaa aatttatttc tgtgggcgca tcgccgggaa
     8281 gagaataacg tcgcgaatag tatgactgtt tgtaaacagc ataatcattc ggtcgatacc
     8341 aatacccaga ccagcggttg gcggcagacc atattccagc gcagttacgt aatcttcgtc
     8401 atagaacatg gcttcgtcgt cacctgcagc tttagcgtta acctgttcct ggaaacgttc
     8461 agcctgatct tctgcgtcgt ttaattcgct aaaaccatta ccgatttcac gaccaccgat
     8521 gaagaattca aaacggtcgg tgatttccgg gttaacatca ttacggcgcg ccagcggtga
     8581 cacttctgcc ggatattccg taataaaggt tggctgaatc agatgtgctt ctgccacttc
     8641 atcaaagatc tccgtgataa tacgtcccag cccccagctt ttctctaccg taattccgat
     8701 agattcagct aatgctttag cagcatcaaa attatccagg tcggccatat cggtttctgg
     8761 acgatatttt ttgattgctt cgcgcatggt gagtttttca aacggtttgc cgaaatcaaa
     8821 cacatgctcg ccataagtga ctttagtggt acccagaacc tcttgtgcca gggtgcggaa
     8881 cagtgactct gtcagttcaa tcaaatcgtg gtaatccgca tacgccatgt agagttccat
     8941 cattgtgaac tcaggattat ggcgaacaga aataccttca ttacggaagt tacggttgat
     9001 ttcgaatacc cgttcaaaac cgcctacaac cagacgtttc agatacagct ccggcgcgat
     9061 acgcagatac atatctaaat ccagagcatt atgatgggta ataaacgggc gagcagatgc
     9121 cccacctgga attacctgca tcatcggggt ttctacttcc ataaagccgc gcgcgaccat
     9181 gaactgacgg atagcggcca gaatttttga acggacaaca aacgtttgac gggatttatc
     9241 gttagcgatg aggtccagat aacgttgacg ataacggact tcctgatcct gcagaccatg
     9301 gaatttatct ggtaaaggac gtagtgcttt agtcagcagg cgcaactcag tacagtgaat
     9361 ggaaagctca cccgtttgcg tcttaaacag cgtaccgcgg gcaccgataa tgtcacccag
     9421 atcccatttt ttaaactgat cgttataaac accttctggc aggctatctc ttgcaacgta
     9481 cagttgaata cggccaccga catcctgcaa cgttacaaag gaggctttcc ccatgatacg
     9541 acgggtcatc attcggccag caaccgagac ttcaatgttt aaggattcca gttcctggtt
     9601 attcttcgca tcaaactctt cgtgcaactg gtcagaggta tggtcgcggc gaaaatcatt
     9661 gggaaacgcc acaccttgct gacgtagtgc cgccagtttt tcgcggcgat ttctcagttc
     9721 atcgttaaaa tcaatagcct cattggctcc ccgtgtttct tgttcagaca ttttggttcc
     9781 tctaaatcca gctttcaatt tagcgtagat aaagagacag atcggtacac tgtattatct
     9841 gcctcgacta ttaataactc agtttatctt atcgaaacta acgcttacct caagttgata
     9901 tcactaataa ataaccatca aaatggttta aatgacttat taatctcgat ttactagtgt
     9961 gattaactga aagttaattt attcataacc aattgttttt acgacagtta atcgttgctc
    10021 tcctgtatca tattcgtcgg cgtgctaaac agaaaacggg tagcaaaggc caatacgacg
    10081 atgatcgcga cacaagccaa cgtccactct cccatctgag aaaagaatcg ctggtaagct
    10141 gcaattacca taccgctaat ttgcgactct gtcgtctgct gtgccacgac gcctgccagc
    10201 cagttggcga ccgcgcctgt tgccagcata taaataccgg ttaatacgcc agacattttc
    10261 agacgcgtga tttgcgcaat cgccaccggg tcaataaaga gttctgcaaa gcccattagc
    10321 gccaacccgg atatcatcac gcccattgac gcttgaccgt cagccgctgc atgtcgggca
    10381 tcaaatgcca acaacataaa gccacaagcc atcagcagta agccaaaggc aaacttcagc
    10441 cagacgcgca atgttgagtt gccgcggctt tctggcgacg ccagccaggc cagtacaacc
    10501 ccagcgagca tcactgcaat tgcattcacc gactggaata gtgctgtagg tacttcaata
    10561 ttgaatgcct gacgattcac aaagcgatcg ataaacaagc tgatggtact gccgccctgt
    10621 tgtgccagta cccagaacaa cgtcccgaca aacatcaaca atacaatttg ccaaagagca
    10681 cggcggtgtt cggggaattt gatcatcatg cgggcaatga tttgtgcggc aatgaggcaa
    10741 acgatcgcca gcaaatatcc ggaccagtcg ttctccagca gcagggtaaa aaatactggg
    10801 gctaaacaga gcatcaccac taaccagctc catactggta aggcaaattt gacgctagtg
    10861 agcgcttttt tatccatact acgcgtggac tggaaatgac gatgaccgct taagaaaatc
    10921 aacaaaccga taaacatgcc gccacccgca agggcaaagc caacatgcca tccataccac
    10981 tgagcagcca ggccgcaggc gattggggcg gcgatagaac cgatattgcc cgcagcatac
    11041 agcagcgaaa aaccgccatc acgtcgatga tcgttctcgt cgtagagctc gccaagcaaa
    11101 cagctgatgt ttgatttgaa taaaccgtag ccacaaataa tgattgccag cgccagatac
    11161 aggctaaagg ttgaatttgt atcaatgccc agcaccacat ggccaagggt cattaacagc
    11221 gcgccggcaa tcactgcagt gcggttgccg agcaggcggt cggcaagcca gccgccgaga
    11281 ataggggtaa cgtaaaccag agaagcatat gcgctgaaca ggctgatggc atggttatca
    11341 tcaaaaccaa gctgatgggt gagatagaga atgagtaagg cacgcatgcc gtaaaaactg
    11401 aagtactccc agatttggat cgccacgata tagtatatcg cgcgcggctg tgagggtgtt
    11461 ttcatgtgtt ctccttatga gcaaaaaagg gaagtggcaa gccacttccc ttgtacgagc
    11521 taattatttt ttgctttctt ctttcaatac cttaacggta tagcggccat cagcctgacg
    11581 gtatgcaccg tgaatatcgg tttcaaatcc cggatagtga gcgccgattt cacacagcat
    11641 ctgcaggaac tccagaaccg gacggctttc ttcggtgatc atttcacccg gcattaccag
    11701 aggaactccc ggcggatacg gaaggatcat attggcgtta atacgaccta ccatttcgtc
    11761 gaggtaaact tcttcggtc
//
LOCUS       DAZPDU010000135        11699 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZPDU010000135 DAZPDU010000000
VERSION     DAZPDU010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11699
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.136"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            197..301
                     /locus_tag="ACHURS_003257"
     CDS             197..301
                     /locus_tag="ACHURS_003257"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFR1238913.1"
                     /translation="MNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            complement(350..1603)
                     /locus_tag="ACHURS_003258"
     CDS             complement(350..1603)
                     /locus_tag="ACHURS_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1238914.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            1710..2603
                     /locus_tag="ACHURS_003259"
     CDS             1710..2603
                     /locus_tag="ACHURS_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1238915.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPASRNFRIHLLNALR"
     gene            2738..3958
                     /gene="mlc"
                     /locus_tag="ACHURS_003260"
                     /gene_synonym="dgsA"
     CDS             2738..3958
                     /gene="mlc"
                     /locus_tag="ACHURS_003260"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFR1238916.1"
                     /translation="MVAENQLGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            4083..4778
                     /gene="bioD"
                     /locus_tag="ACHURS_003261"
     CDS             4083..4778
                     /gene="bioD"
                     /locus_tag="ACHURS_003261"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFR1238917.1"
                     /translation="MLERFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEVIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(4731..6023)
                     /gene="clcB"
                     /locus_tag="ACHURS_003262"
                     /pseudo
     CDS             complement(4731..6023)
                     /gene="clcB"
                     /locus_tag="ACHURS_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
     gene            complement(6182..6796)
                     /gene="dmsD"
                     /locus_tag="ACHURS_003263"
     CDS             complement(6182..6796)
                     /gene="dmsD"
                     /locus_tag="ACHURS_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFR1238918.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(6839..7693)
                     /gene="ynfH"
                     /locus_tag="ACHURS_003264"
     CDS             complement(6839..7693)
                     /gene="ynfH"
                     /locus_tag="ACHURS_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFR1238919.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFMASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(7695..8312)
                     /gene="ynfG"
                     /locus_tag="ACHURS_003265"
     CDS             complement(7695..8312)
                     /gene="ynfG"
                     /locus_tag="ACHURS_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFR1238920.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTSDTTGYLANPEEV"
     gene            complement(8323..10746)
                     /gene="ynfF"
                     /locus_tag="ACHURS_003266"
     CDS             complement(8323..10746)
                     /gene="ynfF"
                     /locus_tag="ACHURS_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HFR1238921.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSYTVNCGSRCLLRLHVKDDTVYWVEYDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEVIHPLPAYTPDFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            complement(10807..>11699)
                     /locus_tag="ACHURS_003267"
     CDS             complement(10807..>11699)
                     /locus_tag="ACHURS_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HFR1238922.1"
                     /translation="ADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPI
                     YWILSEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRK
                     DPNGHFVAYKAFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYAS
                     TFEGWDSPERSTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIAT
                     GDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLR
                     PSPLAKGNPQHTNLVEIEKI"
BASE COUNT         2959 a         2963 c         3021 g         2756 t
ORIGIN      
        1 cagacttccg tgagtggttg gtttcagcgt gtaacgatat gtacaaacgc tgaataaatt
       61 acagcattga taataacgta ttgtgactta agggaaattt agctaccaat aatagtagtc
      121 ttgatcgggt taactatttt actacttaca aagcggtaga ataactgcgc atcagtaata
      181 aatgacacac agcaaaatga atccgtttat ttgggtactt ataatcctga tgacgctaga
      241 cgcgctgcgg gaattggctg gcgcttcgtc tattttagga tggctattaa cgttggtttg
      301 agcaagcaat aagtccggac gtgtagttac cgcagtccgg gcttattttt caggcgtgca
      361 gacgacgatg caaacgcgtc ccgaccagca gagcaatgac cagcatcaga gcaataaatg
      421 cgccgacgcc gttccagcca tagttatgcc agaaaacacc acccagcgtc ccggcaatac
      481 tcgaccccag atagtaactg aacagataca gcgaggaggc ctggccttta gcgcgttttg
      541 cgcgggggcc aatccagctg ctggcaactg agtgggctgc gaagaatcct gctgagaaga
      601 gtaacattcc ggcaaagatc agccacagcg agctgaataa ggtcatcagt aaaccaaaca
      661 gcataacccc cgtcgaaaac aacatcaccg gaccgcgccc gtagcgggtg gtcatggttc
      721 cggctttggg tgagctccat gtaccggtca aataagccag cgataataag ccaaccacgg
      781 cctgactgac atgccagggg gaaagcatca accgatagcc gatgtaatta aacagcgtga
      841 cgaacgaccc catcagcaaa aagccttctg cgaacaataa cggtaatccc cggtcacgcc
      901 agtgcagacg aaagttgata aataacgtct tagggcgcag cgaagtcggg cgaaaatggc
      961 gtgattccgg aaggattttc cagaacatca acgccgaggc tagcgcgaaa caaccgattg
     1021 ccgccagagc aattcgccag ttgaaaaagt ccgtgaagac accactaatt aagcggccgc
     1081 tcatgccgcc aattgagttg ccgctgatat acaaccccat cgaaaaggcc acgaaactgg
     1141 ggtggatttc ctcgctaaga taagtcatgc caacagctgc cacgccactt aacgaaagcc
     1201 caatcaaggc gcgcataatc aaaatgccgt gccagctggt catcattgtc gaaagtaacg
     1261 tacaaatgga ggccaacagt agcgccgtga ccatcactgg tttgcgacca atggcatcgg
     1321 atagggggcc agtaaacagc aaaccaatag ccaacatcgc cgtggaaatg gacagtgaaa
     1381 tactactgtt cgcgggggtt aagccaaact cctgcgaaag caccggaagg ataggctgca
     1441 cacaatagag gagtgcaaag gttgccagtc cggcagagaa cagcgccagg gtgacgcgca
     1501 taaattgcgg cgtaccgcgt ttaataaatt gatttggctg agaaatgctt tgcttgtcag
     1561 tgtcgcttgc cggagcgcca tcaacagttg tagtacggct cacttgaaat ccttgctaaa
     1621 tatgcctgtg gatcaggctt atacataggt taggaaaatc gaattgttct gtctaatata
     1681 ttaataatct caaataagat gttttaaata tgaatattga acttcgtcat ctgcgttact
     1741 ttgttgctgt tgcggaagag ctgcatttcg ggcgcgccgc tgcccgcctg aatatttcgc
     1801 aaccaccgct aagtcagcag attcaggcac tggagcaaca aattggtgcc cgactgctgg
     1861 cacgaactaa tcgcagtgta ttgctgacgg cagcaggaaa acagtttctt gcagatagtc
     1921 ggcaaatcct gtctatggta gatgacgctg ccgctcgcgc tgaaagactg catcagggtg
     1981 aagcggggga gttgcgcatt ggttttactt cgtccgctcc ttttattcgg gcggtgtccg
     2041 atacgttatc gctgtttcgc cgtgattatc ccgatgtcca tttacaaacc cgcgaaatga
     2101 acactcgtga gcaaatcgct ccgctcattg aaggaacgct ggatatggga ttgctgcgta
     2161 acacagcgtt accggagacg cttgaacacg cagtcatcgt ccatgaaccg cttatggcga
     2221 tgatcccgca cgatcatccc ctggcaaata acccgaatgt aacgctggct gaactggcga
     2281 aagaaccctt cgtctttttt gatccgcacg tcgggacagg gctgtatgac gatattctcg
     2341 ggctgatgcg acgttaccat ttgacgcccg tcatcactca ggaggtgggc gaggcaatga
     2401 ccatcatcgg tctggtttcc gccggtctgg gtgtttcaat tttgcctgct tcatttaaac
     2461 gtgttcagct caacgaaatg cgctgggtgc cgattgctga agaggatgcg gtttctgaaa
     2521 tgtggttggt ctggccgaaa catcatgaac aaagtccagc ttcgcgtaac tttcgtattc
     2581 atctgctgaa tgctctcagg tgagggaaat ttcagcgaaa aagcccgaaa aatgtgctgt
     2641 taatcacatg cctaagtaaa aatttgacga cacgtattga agtgcttcac catagcctac
     2701 agattatttc ggagcgcgaa aatataggga gtatgcggtg gttgctgaaa accagcttgg
     2761 gcacattgat caaataaagc agaccaacgc gggcgcggtt tatcgcctga ttgatcagct
     2821 tggtccagtc tcgcgtatcg atctttcccg tctggcgcag ctggctcctg ccagtatcac
     2881 taaaattgtc cgtgagatgc tcgaagcaca cctggtgcaa gagctggaaa tcaaagaagc
     2941 ggggaaccgt ggccgtccgg cggtggggct ggtggttgaa actgaagcct ggcactatct
     3001 ttctctgcgc attagtcgcg gggagatttt ccttgctctg cgcgatctga gcagcaaact
     3061 ggtggtggaa gagtcgcagg aactggcgtt aaaagatgac ttgccattgc tggatcgtat
     3121 catttcccat atcgatcagt tttttatccg ccaccagaaa aaacttgagc gtctaacttc
     3181 gattgccata accctgccgg gaattattga tacggaaaat ggtattgtac atcgcatgcc
     3241 gttctacgag gatgtaaaag agatgccgct cggcgaggcg ctggagcagc ataccggcgt
     3301 accggtttat attcagcatg atatcagcgc atggacgatg gcagaggcct tgtttggtgc
     3361 ctcacgcggg gcgcgcgatg tgattcaggt ggttatcgat cacaacgtgg gggcgggcgt
     3421 cattaccgat ggtcatctgc tacacgcagg cagcagtagt ctcgtggaaa taggccacac
     3481 acaggtcgac ccatatggta aacgctgtta ttgcgggaac cacggctgcc tcgaaaccat
     3541 cgccagtgtg gacagtattc ttgagctggc acagctgcgt ctcaatcaat ccatgagctc
     3601 gatgttacat gggcaaccgt taaccgtgga ctcattgtgt caggcggcat tgcgcggcga
     3661 tctactggca aaagacatca ttaccggggt gggcgcgcat gtcgggcgca ttcttgccat
     3721 catggtgaat ttatttaacc cacaaaaaat actgattggc tcaccgttaa gtaaagcggc
     3781 agatatcctc ttccctgtca tctcggacag catacgtcag caggcccttc ctgcgtatag
     3841 tcagcacatt agcgttgaga gtactcagtt ttctaaccag ggcacgatgg caggcgctgc
     3901 gctggtaaaa gacgcgatgt ataacggttc tttgttgatt cgtctgttgc agggttaaca
     3961 ttttttaact gttctaccaa aatttgcgct atctcaattt gggccaggaa agcataactt
     4021 agactttcaa ggttaattat tttcctagtt tatatttgtg aagcataacg gtggagttag
     4081 tgatgctgga gcgtttcttt attaccggta cagacacttc tgtagggaaa acggtggttt
     4141 cccgcgcatt gctacaagcg ttagcctccc agggaaaaac agttgcggga tataaacccg
     4201 tagcgaaggg gagcaaagag acacccgaag ggctgcgtaa taaagatgcc ctggtgttgc
     4261 agagtgtttc aaccatcgaa ctgccttatg aagcagttaa tcctatcgcg ttaagcgaag
     4321 aagaaagtag cgtggcgcac agttgcccaa tcaattacac cctcatttca aacggcctgg
     4381 caaacctgac cgataaagtc gatcatgtcg tggtagaagg gactggcggc tggcgcagtc
     4441 tgatgaatga tttgcgtcca ctctctgaat gggtggtgca ggaacagttg ccggtgttga
     4501 tggttgtcgg tattcaggaa ggttgcatta accatgcact gctaacagct caggcgattg
     4561 ccaacgacgg gctgccgctc attggctggg tggctaaccg aatcaaccca ggactggcgc
     4621 attatgcgga agtcattgat gtgctgggta aaaaacttcc ggcaccgctc attggtgaac
     4681 tgccttatct gccgcgcgcc gaacagcgag aactggggca atacatccgc ttagctatgc
     4741 tgcgcagtgt gctggcggta gatagagtca cggtgtaacg tccgcgaaat taccgacgca
     4801 atgacgcagg caatcaataa accggggagt agctgatact ccccggtcat ttcacatatc
     4861 atcaacgtcg acataatcgg cgcgtgcgtg gttgccgcca acagtgtcgc catcccggtc
     4921 aatccgagta aaagcgtaat ttcttcgcca tcagggaacc ataatcccag gctacgacca
     4981 tacaacatgc caatggcaag accgataaat agcgtcggtg taaagacacc accgggtgcg
     5041 ccggaaccgc tactcgccag cacggcacac agtttacaaa ggaagatccc ggcaatgatc
     5101 attaacagtg gtggggcggt taaaaaggat tgtacggtgc tatagccgtt gccccacact
     5161 gcaggtgtga acagggaaag cagacccacg atcaacccgc ctagtgccag ttgctagggc
     5221 ggcgcaagtt tgagactcac aaatccacga tgacaggcgt tcattaacgt taacaacagt
     5281 ggtccacaca gacctgccag cacacctgta ctgataatca gcgcatagtc actagcctga
     5341 accgttactg agagttgtac gctgtagagt aacgcgtcgc tatgattaat aagattgcta
     5401 accagcaacg ccacgacggc ggaaataatc accgggccga gagaggccaa catcatagtg
     5461 ccaaacagca cttcggctat aaataaactg ccagcaagcg gggcgcgata ggccgcagcc
     5521 attcccgccg cggccccaca ggcgatccat aatttccatt cctggcgtgg cgtaaaacgt
     5581 tgggcaaaac aggaggcggc aagggcagct aaaagaatca tcgcaccttc gcgaccaatt
     5641 gcactgccgc tggttactac cagcagcgag gcaagcgatt taaccaggct tgctgcgtaa
     5701 tcgaactgtc catcggtttg caacgcttcc atgtaatcgg tcggcgcatg agggcgttgt
     5761 tgggtaaatt tctgccagcc catcagcaac aaacccgccg ccagtccgcc gagcgccgga
     5821 gttagcagcc gtcgccaggg ggaaaggttt gtcgctgcat tgaccagact gccggagtca
     5881 ttattgagga acaaccactc cagtagcagc atcgcatgac gaaacccggc aacggcaaag
     5941 gccgcgagaa taccgacgac tgttgcgata agcagacggc ggaacatagt gcgcagatcc
     6001 ggatagatgt gtagatgatg catgagcaga aatgggcaat gaaacacggg cgactatttt
     6061 gcggcaaaag aggccaaccg caaaagcagt gcgtgcttta agagataaaa aaacgtgaca
     6121 ttgtaactta ttattgccga atcgcgcgta atcgtcacca tccggcaata ttacggtgat
     6181 cctatcgaaa gagtggttta accgcgacag gaatcaacag ttgtgattgc cattgcgcca
     6241 gcgttaagcg agccagttca cccagtgcac ggtagaaagg gtgttctgct ttttcgataa
     6301 aaacatcaag aaaacgtgtt gaccacggaa aaaggtgcca tgccagcaat tcttcgcatt
     6361 ctgtctggcg accattctcc gccaaccacg cagccatcaa cagcagtgaa ccaaaatgat
     6421 cttccggttc gttttgcttc atttcaaact gaatgccttt ctcgcgcatc cactgacgaa
     6481 gtgccaatgt tgaatcgcca aacagcacag attcgcgatc cagccagacc gatccccacg
     6541 gcggagacgg cagtgcccac gggccgacaa acaaacgctg ccaggcctgg gcgtgagtct
     6601 cttcacactg tgtctgaaaa gccgtcacca gcggcgctaa tgacgcttct ggtagaggcc
     6661 actgagtttc ccagccgtca ctggtgagta ccgcaaccag aggtgcggct tccgcactct
     6721 ctggagcgta ataaaacagc gcgcccaaca cgcgcgccgc gacagaaaaa ttatcttgct
     6781 gtgaaaaatg ggtcattcct aacatcctga aatgtgcggg ttgccccgca ccaatttgtt
     6841 aacctgcaat agccattcct actgtcatgt gcaggccata aaagagcaca cgcccgatca
     6901 tctcaccaac aagtatgagg attagcccca gaacaagtcc ggcaacgtga ggttcacggc
     6961 gacgaatgag cggacaaagc cagcatccaa gacctgcgca taacaatacc acacgccaga
     7021 cctgtaagga ggcgtaatct ggtaccagtg cgctggcttg ttgcacggat gagtgaatag
     7081 acgccagaga caaaccttgc agcacaatca ccccggcaca agcgatcagc gcgagaacgc
     7141 taatgatggc aaatggtgtg gtattaaatg taacgcgggc tgcccgcaga attgcggcag
     7201 caagtattgg accgctcaac aataccgtca ggaagaaagc cagcgtagtg taaccgttat
     7261 gccaggttgg cacagtgtcg atttgataca cgcaggtcat catccagacg aaaatgacgc
     7321 cgagcgccat gctgacaagt aaccagagtt tccccaacgc ttgcggcatt ttaccgatga
     7381 ccgccaccag ccaccatagt ccgccaacgg caaaaaatat ggaaccggca gcaatctcgt
     7441 tactcagacc agaagctccg attcgattca gcgagttaaa cgcccgcagt ggcgatccca
     7501 ggtgcatgac agaggccata aacccgatgc ccatcaacag ccagaggaaa aacatgccac
     7561 ggacaatacg ctgacggtct gcgtcatttt tggcggcgaa ccagccaatt ccgctaacaa
     7621 tcagcgcgcc tactacgcat tgacccaata cggtaaagat aaccagtggc cactcatgcc
     7681 atccatttcc cattttacac ttcctccgga ttagccagat aaccagtggt atcgctggtc
     7741 gggcggctgt tggcgttagg tttgataacg atattgggtt ttgtgaaatg cgcgcgcggc
     7801 agtggcgcga cggcagccag agtgccgtgc ttctggcgca gctcttcaat tggaccgaac
     7861 tcaagtgcac gcagcgggca ggattcgaca catatgggtt gtttcccctc ggcgacgcgc
     7921 gaataacaac catcgcactt cgtcatgtgc cctttttcag cattgtactg tggcgcgccg
     7981 tacggacaag ccatgtggca gtagcggcag ccgatacaaa catcttcatc aaccaccaca
     8041 aagccatctt cacgcttatg cattgcgccg ctggggcaga cttttgtaca tgcggggtca
     8101 tcgcaatggt tacaggagat ggagagataa taagcgaaca cgttctggtg ccagacgcca
     8161 ttatcttcct gccagtcgcc gccagcatat tcataaatac ggcggaaact gacttccggg
     8221 ccaagatctt taaaatcttt gcacgccagt tcgcaggttt tacagccagt acagcggctg
     8281 gaatcgataa aaaatccata ttgtgtggtc acgggctact ccttaaacct tttcgatctg
     8341 gacaagattg ctgtgcgacg ggtttccctt tgccagcggt gaagggcggt gagaggtcag
     8401 aatattgata ctgccgccgt gatcgacccg gtcaccaaac atatccgctt taagccaagc
     8461 accttgcccg atggcggtaa cgccaggcag aatacgcgga gtcacttttg cggcaatcag
     8521 catttctcca ttattgttaa atacccgcac ggtatcgcca tgacggatac cgcgtgcctg
     8581 agcatcaatg gggttgatcc acacctcttg tgggcaggcc tgctgtaaca catcaatatt
     8641 gccgtagctg gagtgggtac gcgctttgta atggaagccc gttaactgca gtggataggt
     8701 tttccgcagg ggatcgtccc agccatcaaa atctggggta tacgcaggaa ggggatgaat
     8761 aacttcatct tttttcaatt cccaggtatc cgcaatcttc gccagtcgtt cagaataaat
     8821 ttcgattttc cccgaaggtg ttttcaacgg gtttgcctgt ggatcttcac ggaatgcgcg
     8881 gaaagcgacg tagtgttctt ccgggcattt tttcttaaag atcccggtcg ttttcatctc
     8941 ctcgtagtcg ggcatctcag ggttacgttc cttcgttttc gcatggagat atttgatcca
     9001 ttcatgctga ctgcgacctt cagtaaaggt ttgataaacg tctggtccta agcgtttggc
     9061 gacttcactc agcatccagt agatgggttt gcgttcaaat tttgctgagg ttgcgggttg
     9121 ggcgaggatc acatagccca tattccctgc agattcatga gagataaggt cttcttgctc
     9181 tgttggcatc aggtcgggca acaggatatc gcaatactta gccgaggccg tcatgaagtg
     9241 gtcaatgcca acaatcatct cgcacttgct gtcatcctga agcacctcat gggtgtgatt
     9301 gatgtcgcca tgttgattga tcaatgtgtt actggcgtag caccataaaa acttgatggg
     9361 gacatccagt ttttcttttc cacgaacacc atcacgggtc gcggtcattt ccgtaccatg
     9421 gtcgatggca tcagtccatg taaagacgga aatctgcgtt ttaacaggat tctcgagcat
     9481 cgggaaccat tctaccccca gatcccagct accttcgcgt acgcctgagt tgccgccgtt
     9541 tatgccgacg ttaccggtga gaacggaaag catggcaata gcgcgggatg tttgttcgcc
     9601 gttggagtgt cgttgtggcc cccaaccctg acaaatataa gcaggttttg ctgaaccgat
     9661 ctctcgtgcc aactggataa ttttttctgc cgggatgctg gtgatttttg ctgcccattc
     9721 cggcgtttta gctatgccgt caggcccttc gcccagaata taggctttat aatgcgcgtt
     9781 acgtggtgcg ttggcgggca gcgttttttc atcgtaacca acacaatatt tgtcgagaaa
     9841 tggctgatcg accatgtttt cagtaatcag tacccaggca atcgcacagg ccagtgcgcc
     9901 atcggtgcca gggcgaatgg gcagccattc atcttcacgc ccggcagctg tgtcgttata
     9961 acgtggatcg atgacgatca tgcgtgcgtt tgaacgttcg cgggcttgct cgacgtagta
    10021 agtgacacca ccaccgctca tccgcgtttc tgccgggtta tttccgaaca taacgaccag
    10081 tttcgtattg gcgatatcat ccgggctgtt gccatcattg gcaccgaaca tataactcat
    10141 tgcggcactg atctgtgcgg tactgtagct gccatagcga ctgagaaaac caccgcaaga
    10201 gttcatcaga cggtacggga cgtttgagtt ggtgatgttt ccgccatcta cacctgttcc
    10261 gtacaggaca tgtacagctt cattgccgta atcttttagg atccgccgaa gattatcact
    10321 gatggtatcc agggcttcgt cccaacttat ccgttcaaat ttaccttcac cgcgcttgcc
    10381 gacgcgcttc atgggatatt tcaacctatc aggatgattc atccgtcggc ggatagagcg
    10441 cccgcgtaaa cacgcgcgaa cctgatgatt accgtagacg tcgtcacctg tcgtatcata
    10501 ctccacccag tacacggtgt catctttcac atgcaaacgt aacagacagc ggctcccgca
    10561 gttaacggtg taggaactcc agaccgcttt ctcttctacc ggagcctctg ccgcccgaac
    10621 catttgggaa aatggcagag tgaaagcact gcttgccagc gcaaggctgc caagtgcgga
    10681 ggttttcatc agacttctac ggctgatttc agccttcatg agcgcctctg tggtatggat
    10741 tttcatcatt actcacttat tgcttttcaa acaaaatgtc atgccagaat ttatggttgt
    10801 agtgggttat attttttcga tctcgaccag attagtgtgc tgcgggtttc ccttcgccag
    10861 tggtgaaggg cgcagagtgg ttagcgtatt cacacagccg ccatggtcga ttttatcgcc
    10921 agacatattg gcctcgtgcc aggctccctg gcccatagcg ctaaccccag ggagaatgcg
    10981 tggtgttact ttggctggta gccgaacttc gccacgatgg ttaaacaccc gcaccatatc
    11041 gccggtggca atcccacgtt tctgtgcatc tatagggttg atccacactt cctgacggca
    11101 ggctgccttc aggacatcaa tgttgccgta ggtcgagtga gtacgggatt tgtaatggaa
    11161 accaaacagt tgcagtggga aggtactacg ttcaggggag tcccagcctt caaaggttga
    11221 ggcataaact ggcagtgggc ttatcacttc atctttttcc agttcccagg tacgggcaat
    11281 ttccgccagc ttgctggaat aaatttcaat cttaccggaa ggcgttttaa gtggatttgc
    11341 ctcggggtcg tcacgaaatg ctttgtaggc gacaaaatga ccattgggat ctttacgctt
    11401 atagataccc atttttttca gttcgtcgta agacggtaac gccggatctt tggcaagcat
    11461 tttggcgtac agatgttgta accattgttc ctgcgtgcga ccttcagtga acttttgata
    11521 gacgtcaggt ccaagacgtt tcgcgacttc actcaggatc cagtaaatcg gtttgcgttc
    11581 gaatttttcg ctggtgacag gctggaggaa aatgagatat cccatgttac cggcgtagtc
    11641 gttaggaata atatcttcct gctcaacggt catcaggtct ggcagcagaa tgtcggcat
//
LOCUS       DAZPDU010000136        11542 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZPDU010000136 DAZPDU010000000
VERSION     DAZPDU010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11542
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.138"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            460..1386
                     /locus_tag="ACHURS_003268"
     CDS             460..1386
                     /locus_tag="ACHURS_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase DNA-binding-containing protein"
                     /protein_id="HFR1238923.1"
                     /translation="MENWVIQELKSLDIGDSRLDKRVKHILNSLSRSPEESIPVSCRT
                     WSETKAAYRCFSNDKVSANKIMAPHKENITERAQQFKRVLVLQDTTELNYSGQKEKQG
                     VGHKKHKDERNLFLHPQLVISESGVCLGVYDDYQWFRDELKTSKNTRQEICNDLLHKK
                     HVSEKETWRWVEGYNKATELARQCPDTHVLSISDREGDFYDLFERAEQTPGIKADWLV
                     RMKFNNRATLNINGKRDHRLLHERIMEITPQQLVEFTIPDGRGQQARTLPDMLSSDAG
                     PLSYPLIYSSKVMIISLFDSTVMPDKYRHNGK"
     gene            complement(1336..2529)
                     /locus_tag="ACHURS_003269"
     CDS             complement(1336..2529)
                     /locus_tag="ACHURS_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1238924.1"
                     /translation="MSPGIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFVTDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2665..4389
                     /gene="iucA"
                     /locus_tag="ACHURS_003270"
     CDS             2665..4389
                     /gene="iucA"
                     /locus_tag="ACHURS_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HFR1238925.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            4390..5337
                     /gene="iucB"
                     /locus_tag="ACHURS_003271"
     CDS             4390..5337
                     /gene="iucB"
                     /locus_tag="ACHURS_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase IucB"
                     /protein_id="HFR1238926.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            5337..7079
                     /gene="iucC"
                     /locus_tag="ACHURS_003272"
     CDS             5337..7079
                     /gene="iucC"
                     /locus_tag="ACHURS_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIS family aerobactin synthetase IucC"
                     /protein_id="HFR1238927.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFVEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDATSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            7076..8413
                     /gene="iucD"
                     /locus_tag="ACHURS_003273"
     CDS             7076..8413
                     /gene="iucD"
                     /locus_tag="ACHURS_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase
                     IucD"
                     /protein_id="HFR1238928.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFARNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            8419..10614
                     /gene="iutA"
                     /locus_tag="ACHURS_003274"
     CDS             8419..10614
                     /gene="iutA"
                     /locus_tag="ACHURS_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HFR1238929.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLSLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYCPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(11165..11308)
                     /locus_tag="ACHURS_003275"
     CDS             complement(11165..11308)
                     /locus_tag="ACHURS_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1238930.1"
                     /translation="MEHRSVIHGELAIDHDAILQCAEEVLHHCVIQTVSFVWHILCYS
                     MGL"
BASE COUNT         2926 a         2896 c         2950 g         2770 t
ORIGIN      
        1 ctcaacgaag gggccgacaa acacgttgtc cctgagttca cattcataaa tgttgaccgg
       61 tttgacaata cggacattga tcccataaat cacatcccgg acagcacatt ccagaaattt
      121 catactcaca cgtcctttaa atacagatga catttgccgg aattataccg cccagattca
      181 cttgccatgt ttcagaatag cttataagcc gcacttttac cgatactcag caacaaatca
      241 ggtctgtatc cacttcagcc ggaaaccgga tctgcgcggc ggggaacggg caagaaatat
      301 gataattaag caacacacat aaaatcatat tgcataatcg catctgaatc gaacacttat
      361 tcgcatcact atcgatcatc acttcgcatc tcataccctt acccacaagt tgaaataata
      421 gtattatatt gaaaagacag agattatttc agaggtgtta tggaaaactg ggttattcag
      481 gagctaaaat cactggatat aggggactca agacttgata aaagagtaaa acatatcctc
      541 aatagcctca gtcgttcacc agaagaaagt attcctgttt cctgccgtac ctggagtgaa
      601 acaaaggcag cttatcgttg tttttcaaac gataaagtct ctgctaataa aattatggct
      661 cctcacaaag aaaatatcac tgaaagagct cagcagttta aaagagtttt agttttacag
      721 gatacaacag aacttaatta ttctggtcag aaagaaaaac aaggagttgg tcacaaaaaa
      781 cataaagatg aaagaaactt atttcttcat cctcaactcg tcatcagtga gtcaggtgtt
      841 tgcctcggtg tttatgatga ctatcagtgg ttcagggatg aattaaaaac cagtaaaaat
      901 acccgacaag aaatctgcaa tgatttactt cataaaaaac atgtcagtga aaaagaaacc
      961 tggcgttggg ttgaaggata taataaagca acagaactgg ctcgccaatg ccctgatacc
     1021 catgtattgt caatttctga tcgtgaaggc gatttttatg acctgtttga acgcgcagag
     1081 cagactcccg gaataaaagc tgactggctt gtcagaatga aatttaataa cagagcaacg
     1141 ctgaatatta atggtaaacg ggatcatagg ttgttgcacg aaagaatcat ggagattaca
     1201 ccgcagcaac tggttgaatt cactatacct gatgggcggg ggcagcaagc cagaacactg
     1261 cctgacatgc tttcatcaga cgcaggtcct ctctcatatc ctctgattta ctccagtaag
     1321 gtgatgatca tatctttatt tgatagtacg gtaatgccag ataaatacag acacaatggt
     1381 aaataatata gttgcaaata atgctgccat aatcagcgcg attgcgacac ctgttttcga
     1441 taccagcggc accagcaaag cgcacagtcc atagccaagt gtgtgactgg tagcgagcca
     1501 gcccgcgcct gcgccatcag ccagcttatc attcagtaga agttgataag cgggtgttgc
     1561 cagagctgct ccaaatgaca gcactgcaca gcccgggtaa aacaaccata gctgttcgga
     1621 gagcatgata gccagaccac cactcatcaa cactccggca ctcaggagca gggccaccgg
     1681 agtcaggcgc tgcggacgga gtaccccgaa ctgcgcgata agcgcagcta ctgcggacag
     1741 acccaacagc caggccacct gttggctaat ggcggtggta tccgtgacga actggcgagt
     1801 aagggcaggc gaaagtccaa gctgcatcat gctcaccgcc gcagccagta atattgcgca
     1861 aagcagataa ggcagacaat cccgcttcag actgacgctc ttgcactccg gtgtgggctg
     1921 tggtggtgtg ccgggcaacc gcagcagcat cagcagcgcc agcactggtg ctgccatcag
     1981 tagccccagt ggcgccagag gatgaatggc cagcattgct gccgcgcaca gcggcccgaa
     2041 tagcctgccg caactcaggc cggagctgat ggttgccaga gcggccatgc gattcccttc
     2101 tccagctctc tgcaatgccc agacctgaca ggctggcacc attgccgaca cggtcagccc
     2161 gtaggcgatc cgggcgatga ttaatattcc cagtcccacc gctgttgtta tggcgctggt
     2221 agccatcagc acgcttccca gccctagcag ggtaaagctt atgccatatc ctcccagagc
     2281 ccacaacacc acaggtttac tgccagtacg ggaaatttgc tttccccacc acggggacga
     2341 tggcagaaac agcattgagc caagcatcag cagggcagcc catacagaca gactcagatt
     2401 tgtctgtatg accagaacag ggagcacaac cagcagaccg ttctgtccga taccgagaag
     2461 cccggcactg aacgcaagtg gccagcagga cagtgttttt tggggcgtat cttcgatccc
     2521 tggtgacatt ataatgtttc aactccatgt attaattgtg tttatttgta aaattaattt
     2581 atctgacaat aacatttatt gttgataatg agaatcatta tcgacataat tgttattatt
     2641 ttactgtgtg ggagctgttt gactatgacc ctgccctctg aaaaaccagc cacagatgtg
     2701 gctgcgcagt gcttcctgaa tgcactgatt cgtgaaacca cagactggaa actgacagaa
     2761 tacccgccag acgaattgct catcccgctg gatgagcaga aatcgctcca tttcagagtg
     2821 gcttatttct ccccaaccca gcatcaccgc tttgcatttc ctgcacgtct ggtcacggca
     2881 tcaggcagtt atcctgtcga ctttaccact ctctcccggc ttattattga taagctacgc
     2941 catcaactgt ttctgcccgt tcccctctgc gaaactttcc accagcgcgt gctggaaagc
     3001 catgtccata cgcaacaggc aattgatgcc cgtcatgact gggccgctct gcgtgaaaaa
     3061 gcgttgaatt ttggcgaggc tgagcaggcg ctgctgacag gacacgcttt ccaccctgcg
     3121 cctaagtctc atgaaccgtt taaccgacgg gaggctgaac gctacctgcc tgatatggca
     3181 ccccacttcc cactgcggtg gttttcggtg gataaaacgc aaatcgccgg tgaaagtttg
     3241 catctcaacc ttcaacagcg gctgacgcga tttgccgcag agaatgcgcc tcagttactc
     3301 aacgaattaa gtgacaacca atggctgttc ccgttgcacc cgtggcaggg ggaatatctt
     3361 ttgcagcagg ggtggtgcca ggcacttgtt gctaaagggc tgattaaaga cttaggtgag
     3421 gccggcacgt cgtggctgcc gaccacctct tcccgttccc tctactgtgc caccagccgc
     3481 gatatgatca agttctccct gagcgtacgg ctgaccaact ccatccgtac tctgtccgtg
     3541 aaagaagtga agcgtgggat gcgcctggca cgcctggctc aaaccgacgg ctggcagatg
     3601 ctacaggtcc gcttcccgac tttccgggta atgcaggagg atggctgggc cgggctgctc
     3661 gatcttaacg gcaacatcat gcaggaaagt ctgtttgccc tgcgtgaaaa tctgctggtg
     3721 gatcagccga aaagccagac taacgtactg gtctccctga ctcaggccgc acctgatggc
     3781 ggtgattcgc tgctggtttc ggcggtaaaa cgcctgagcg atcgcctcgg tatcactgtg
     3841 caacaggccg cccatgcatg ggtcgatgca tactgtcagc aagtgctaaa gccgctgttt
     3901 acggctgaag cggattacgg cctggtgctg ctggcgcatc agcaaaatat tcttgtccag
     3961 atgcttgggg atctgccggt cggatttatt taccgtgact gtcagggcag cgcttttatg
     4021 cctcacgcga cagactggct cgattctatt ggcgaggcgc aggcggaaaa tattttcacc
     4081 catgagcagt tgctgcgcta tttcccttat tacctgctgg ttaactccac ttttgctgtg
     4141 accgctgcgc tgggggctgc cgggctggac agcgaatcga atctgatggc tcgtgtacga
     4201 gcatcgctgg ctgaagtgcg tgatcaggtg actcataaaa cctgtctcaa ctacgtgctg
     4261 gaaagtccgt actggaacgt aaaaggtaac tttttctgtt atctgaacga tcataacgag
     4321 aacaccattg ttgacccttc ggtgatctat ttcgatttcg ctaacccgct gcaggctcag
     4381 gaggtctgaa tgtctgaggc aaacattatt cacagcagat atggactgcg ctgtgaaaaa
     4441 ctcgacaagc ccctgaatct tggctgggga ctggacaata gcgcggtgtt gcactgtccc
     4501 ggggagctgc cgacagggtg gctgtgcgat gcgctggatc agatatttat cgccgcacca
     4561 caactgtcag cagtggctct cccctgggcc gaatggcgtg aggagccaca ggcgctgacg
     4621 ctttttgggc aggtaaaaag cgacattatc catcgtactg ccttctggca gttaccgtta
     4681 tggttgagtt caccggcaaa ccgggcctcc ggtgaaatgg tttttgatgc agagcgtgag
     4741 atttatttcc cgcagcgccc accccgtcca cagggtgagg tttaccgccg ttacgatcca
     4801 cgtattcgca ggatgctgag tttccgcatt gccgatcccg tttctgatgc agaacgtttc
     4861 acccgctgga tgaatgatcc gcgcgttgag tatttctggg agcaaagtgg ctcactggag
     4921 gtacagaccg cctatctgga gcgccagctc accggtaaac atgcattccc gctgattggg
     4981 tgcttcgacg atcggccatt cagctatttc gaaatctact gggcggcgga agaccgcatt
     5041 ggtcgtcact attcgtggca gccctttgac cgtggcctgc atctgctggt tggtgaacag
     5101 caatggcgcg gggcccacta tgtgcaaagc tggctgcgcg ggctgacaca ttacctgctg
     5161 ctggatgagc cccgtacgca gcgcacagta ctggagccac gcaccgataa ccagcgcctg
     5221 ttccgccatc ttgagcctgc gggataccgg acaattaaag agttcgactt cccacataag
     5281 cgctcgcgca tggtgatggc ggatcgtcat cacttcttca cggaggtcgg tctgtaatga
     5341 atcacaagga ctgggatttt gttaaccgtc ggctggtggc aaaaatgttg tctgagatgg
     5401 agtatgagca ggttttccac gcggaatctc agggcgatga ccactactgc attaacctgc
     5461 cgggagcaca atggcgcttc atcgctgaac gtggtatctg gggctggctg tggattgatg
     5521 cccaaacgtt gcgctgcacg gacgaaccag tactggcgca gacgctgctg atgcagctaa
     5581 agccggtgct gtcaatgagt gatgccactg ttgcagagca tatgcaggat ttgtatgcca
     5641 cgctactggg cgacctgcaa ctgctgaagg cccgtcgcgg actgagtgcc agtgacctga
     5701 ttaatcttga tgccgatcgt ctgcaatgcc tgctgagcgg tcatcctaaa ttcgttttta
     5761 ataaaggtcg ccgtggctgg ggcaaagagg cgctggaacg atatgcgcca gagtatacca
     5821 acaccttcag actgcactgg ctggcggtaa aacgtgaaca tatgatctgg cgctgcgata
     5881 acgatctgga tattcagcag ttgttgactg ccgcaatgga tccgcaggag tttacacgct
     5941 tcagtcaggt ctggcaggaa aacgggctgg atcacaactg gctaccgctg ccggtacatc
     6001 cgtggcagtg gcagcaaaaa atcgccacag acttcatcgc tgattttgtc gaaggcagga
     6061 tggtgtctct cggcgagttt ggcgaccagt ggctcgccca gcagtcgctg cgtaccctga
     6121 ccaacgccag ccgccgggga gggctggata tcaagctgcc gctgaccatc tacaacacct
     6181 catgctaccg gggaatccct ggcagatata tcgctgccgg accactggct tcacgctggc
     6241 tgcaacaggt ttttgcgacc gacgccaccc tggtgcaaag cggcgcagtg atccttggtg
     6301 aaccggctgc aggctatgtg tcccatgaag gctatgccgc gcttgcccgg gctccctatc
     6361 gctaccagga aatgcttggt gttatctggc gggagaatcc gtgccgctgg ctgaaaccgg
     6421 atgaaagtcc tgttctgatg gcaacactga tggagtgcga cgaaaacaat cagccgctgg
     6481 caggcgcata tatcgaccgc tccggactgg acgctgaaac ctggcttacg caattgttcc
     6541 gggtggttgt ggttccgctg tatcacctgc tttgccgcta cggtgtcgcg cttattgcac
     6601 acggacaaaa tataacgctc gccatgaaag agggggtacc acagcgtgtt ctgctgaaag
     6661 acttccaggg cgatatgcgg ctggtgaaag aagcgtttcc cgaaatggac tctttgcctc
     6721 aggaggtgcg tgatgccaca tcccgtctga gtgcggacta cttaatccat gatttgcaga
     6781 cgggtcactt cgtgacagta ctgcgtttta tttcgccact gatggttcgt cttggcgtac
     6841 ctgaaaggcg attttatcaa ctgctggcag cagtgttgag tgattacatg aacaaacatc
     6901 cacaaatggc agagcgtttt gcgcttttct cactcttcag gccacaaatc attcgcgtgg
     6961 tgctgaaccc ggtaaaactg acctggccgg acctggatgg cggcagtcgc atgctgccga
     7021 attaccttga gaatctgcaa aatccgctgt ggctggtaac tcaggaatat gaatcatgaa
     7081 aaaaagtgtc gattttattg gtgtcgggac agggccattt aatctcagca ttgctgcatt
     7141 gtcacatcag atcgaagaac tgaactgtct cttctttgac gaacatcctc atttttcctg
     7201 gcatccgggt atgctggtac cggattgtca tatgcagacc gtctttctga aagatctggt
     7261 cagtgcagtt gcacctacaa atccctacag ctttgttaac tatctggtga agcacaaaaa
     7321 gttctatcgc ttccttacaa gcaggctacg tacagtatcc cgtgaagagt tttctgacta
     7381 tctccgctgg gctgctgaag atatgaataa cctgtatttc agtcataccg ttgaaaatat
     7441 tgatttcgac aaaaaaagtc gattgtttct ggttcagacc agtcggggag aatattttgc
     7501 ccgcaatatc tgccttggta caggaaaaca accttattta ccaccctgtg tgaagcatgt
     7561 gacacaatcc tgtttccatg ccagtgaaat gaatcttcgt cggccggacc tgagtggaaa
     7621 acggataacc gtggttggtg gaggacagag tggtgcagac ctgttcctta atgcattacg
     7681 cggggaatgg ggagaagcgg cggaaataaa ctgggtctca cggcgtaata attttaacgc
     7741 actggatgag gctgcttttg ctgatgagta ttttacacct gaatatattt caggcttctc
     7801 cggactgaag gaagatattc gccatcagtt actggatgag cagaaaatga catcggatgg
     7861 catcactgcc gattctttac tgaccattta tcgtgagttg taccaccgtt ttgaagttct
     7921 gagaaaacca agaaatatcc gtctgctacc cagtcgctcg gtaacaaccc tggaaagtag
     7981 tggtccgggc tggaggttat tgatggaaca tcatctggat cagggcagag agagcctgga
     8041 aagtgatgtg gtgattttcg ccacaggtta ccgttctgca ttgccacaaa tacttccctc
     8101 actgatgccc ttgatcacca tgcacgataa gaacaccttt aaagtgcgtg atgacttcac
     8161 tctggaatgg agtggcccga aagaaaataa catctttgcg gtcaacgcca gcatgcaaac
     8221 tcatggcatc gccgaacccc agctcagcct gatggcctgg cgatctgcac gtattcttaa
     8281 tcgcgtactg gggcgtgatt tattcgatct cagtatgccg cccgcactga ttcagtggcg
     8341 cagcggcagc cggaaaaaac cgcagccgga ggctgcttct ttaactcact atacagcaaa
     8401 tattcaggaa taacgatgat gcgcaaaaag tatatgcccc gggctcttgg tccgctgctt
     8461 cttgtcgtgc tgtcaccagc tgtcgcccag caaaacgatg ataatgagat catagtgtct
     8521 gccagccgca gcaatcgaac tgtagcggag atggcgcaaa ccacctgggt tatcgaaaat
     8581 gccgaactgg agcagcagat tcagggcggt aaagagctga aagacgcact ggctcagtta
     8641 atccccggcc ttgatgtcag cagccagagt cgaaccaact acggtatgaa catgcgtggc
     8701 cgcccgctgg ttgtcctgat tgacggtgtg cgcctcaact cttcacgttc cgacagccga
     8761 caactggact ctgtcgatcc ttttaatatc gaccatattg aagtgatctc cggcgcgacg
     8821 gccctgtacg gtggcgggag taccggaggg ttgatcaaca tcgtgaccaa aaaaggtcag
     8881 ccggaaacca tgatggagtt tgaggctggc acaaaaagtg gctttaacag cagtaaagat
     8941 cacgatgagc gcattgccgg tgctgtctcc ggcggaaatg accatatctc cggacgtctt
     9001 tccgtggcat atcagaaatt tggcggctgg tttgacggta acggcgatgc caccctgctt
     9061 gataacaccc agaccggcct gcagcactcc aatcggctgg acatcatggg aaccggtacg
     9121 ctgaacatcg atgaatcccg gcagcttcaa ctgataacgc agtactataa aagtcaggga
     9181 gacgacaatt acgggcttaa tctcgggaaa ggcttttccg ccatcagcgg gagcagcaca
     9241 ccatacgtca gtaaggggct gaattctgac cgcattcccg gcactgagcg gcatttgatc
     9301 agcctgcagt actctgacag tgatttcctg ggacaggaac tggtcggtca ggtttactac
     9361 cgcgatgagt cgttgcggta ctacccgttc ccgacggtaa atgcgaataa acaggcgaca
     9421 gctttctcct cgtcacagca ggataccgac cagtacggca tgaaactgac tctgaacagc
     9481 caactgatgg acggctggca aatcacctgg gggctggatg ctgagcatga gcgctttacc
     9541 tccaaccaga tgttcttcga tctggctcag gcaagtgctt ccggagggct gaacaaccat
     9601 aagatttaca ccaccgggcg ctatccgtca tatgacatca ccaatctggc ggccttcctg
     9661 caatccagct atgacattaa tgatattttt accgttagcg gtggcgtacg ctatcagtat
     9721 actgagaaca gggtagatga tttcatcgac tacacgcagc aacagaagat tgctgccggg
     9781 aaggcgatat ctgccgacgc cattcctggt ggttcggtag attacgataa ctttctgttc
     9841 aatgctggtc tgctgatgca catcaccgaa cgtcagcagg catggttcaa tttttcccag
     9901 ggggtggcat tgccggatcc ggggaaatat tatggtcgcg gcatctatgg tgcagcagtg
     9961 aacggccatc tttccctgac aaagagcgtg aacgtcagcg acagtaagct ggaaggcgtg
    10021 aaagtcgatt cttatgaact gggctggcgc tttaccggtg acaacctgcg gactcaaatc
    10081 gcggcatatt actcgctttc caataagagc gtggaaagga ataaagatct gaccatcagt
    10141 gtgaaggacg acaggcgccg tatttacggc gtggaaggtg cggtggacta cctgatcccg
    10201 gatactgact ggagtaccgg tgtgaacttc aatgtgctga aaaccgagtc gaaagtgaac
    10261 ggtcaatggc aaaaatatga cgtgaaggaa tcaagtccat cgaaagcgac agcttacatt
    10321 aactgggcgc cggaaccgtg gagtctgcgt gtacagagca ccacttcttt cgacgtaagc
    10381 gatgcagagg gtaacgatat taatggttac actaccgtcg attttatcag tagttggcag
    10441 cttccggtgg gaacactcag cttcagcgtt gagaacctct tcgaccgtga ctataccact
    10501 gtctggggac agcgtgcacc tctgtactac tgcccgggtt acggccctgc ttcactgtac
    10561 gactacaaag gccggggccg aacctttggt ctgaactact cagtgctgtt ctgacgggga
    10621 tgcttttgca acaagggtat gctgtaaaca acatggtcct tggtggccat gttgtttaca
    10681 gccagtataa tatacagcct taacaagata ttccgtctcc ggaatggact gttcaccact
    10741 gaataacatt atttctgttt tatggatctt tgactgaaat ttatcgcata tcctcatttc
    10801 attgtccgtc tgaaaactaa ccttacaagt aagcgtcgtc tcagcaccgt ctggcagatc
    10861 ctgaaattcc tgagaggatg atctgcgccc aatatcctga gccggaactt tgctgcaatg
    10921 aaggccaacg aaaaatgggg gagtatgtta tagagtactt gctacagggt aaaaagaggt
    10981 acctgtcgct ggttctccgt tcagtttaac cgggagattt tctcctgcgc ctgtcataaa
    11041 gtccggtaat ggagattgtt aatacgatgg tgcacgatgc attgtcggtg cccggcccgg
    11101 aggatgcccc gtaactacat acggatcagg gttggcaata tcgaatggca ggctataggc
    11161 aaagttaaag ccccatggag tagcacaaaa tatgccacac aaaggaaacg gtctgaataa
    11221 cgcagtgatg gagaacttct tcggcacact gcaaaatcgc atcgtggtcg atcgctaatt
    11281 cgccgtgaat cactgaacga tgttccatgc gaacagatat tcgatcatat cgaaatatta
    11341 aacattaccc ctccccgcat tgggcaaaaa aaaaacaaaa aacgatcaat tagtattgat
    11401 tcacataatc tctcatcatt aatgagaaca ttgtcacata tttacaatat agaactgtta
    11461 cttttagtaa gctgttagta accaactgtt actaataatt ttaattctta atactttgtt
    11521 cattcaataa atgagttgag ag
//
LOCUS       DAZPDU010000137        11472 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZPDU010000137 DAZPDU010000000
VERSION     DAZPDU010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11472
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.139"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            1..614
                     /locus_tag="ACHURS_003276"
                     /pseudo
     CDS             1..614
                     /locus_tag="ACHURS_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            611..1225
                     /gene="prfH"
                     /locus_tag="ACHURS_003277"
     CDS             611..1225
                     /gene="prfH"
                     /locus_tag="ACHURS_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFR1238931.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1282..2739)
                     /gene="pepD"
                     /locus_tag="ACHURS_003278"
     CDS             complement(1282..2739)
                     /gene="pepD"
                     /locus_tag="ACHURS_003278"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFR1238932.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3000..3458
                     /gene="gpt"
                     /locus_tag="ACHURS_003279"
     CDS             3000..3458
                     /gene="gpt"
                     /locus_tag="ACHURS_003279"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFR1238933.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3550..4794
                     /gene="frsA"
                     /locus_tag="ACHURS_003280"
     CDS             3550..4794
                     /gene="frsA"
                     /locus_tag="ACHURS_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFR1238934.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4852..5253
                     /gene="crl"
                     /locus_tag="ACHURS_003281"
     CDS             4852..5253
                     /gene="crl"
                     /locus_tag="ACHURS_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFR1238935.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5292..6347)
                     /gene="phoE"
                     /locus_tag="ACHURS_003282"
     CDS             complement(5292..6347)
                     /gene="phoE"
                     /locus_tag="ACHURS_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFR1238936.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            6635..7738
                     /gene="proB"
                     /locus_tag="ACHURS_003283"
     CDS             6635..7738
                     /gene="proB"
                     /locus_tag="ACHURS_003283"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFR1238937.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7750..9003
                     /gene="proA"
                     /locus_tag="ACHURS_003284"
     CDS             7750..9003
                     /gene="proA"
                     /locus_tag="ACHURS_003284"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFR1238938.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            9118..9193
                     /locus_tag="ACHURS_003285"
     tRNA            9118..9193
                     /locus_tag="ACHURS_003285"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9151..9153,aa:Thr,seq:cgt)
     gene            9799..9957
                     /locus_tag="ACHURS_003286"
     CDS             9799..9957
                     /locus_tag="ACHURS_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1238939.1"
                     /translation="MKNIAAIKRNNRKIHARKFLSTPEGKAWLERKQRENEERKLLSE
                     LKWLREDF"
BASE COUNT         2972 a         2670 c         2894 g         2936 t
ORIGIN      
        1 agtctatttt acagcggcat attgcctcgt tttcgcatca tggtttgcct gaaggcagtg
       61 acgacgcgct acggtatatt gcggaacatg acgatgcgct ggcgtttgcg cgtattaatc
      121 gccagctgat ctctttacgc ataatgcaac aggttaaggc caccggaagt ccggttctgg
      181 atgtggcgca taactttgtt agcgcgtgtc aaatcggcga tcaacagggc tggttgcatc
      241 gtaaaggtgc cacgccggat gacaacggtc tggtgattat tcccggttca ctcggtgatt
      301 actcctagct tgtacagcct gttgcgaacg aaaaaacgct ccactcgctg gcgcatgggg
      361 ctgggcgtaa atgggggcgc accgagtgta aagggcgtct ggcagcgaaa tacacagcga
      421 cgcagctctc acgtactgaa cttggcagtc gggtaatttg tcgcgataaa caactcatct
      481 ttgaagaagc gccacaagcc tataaatcgg ctgaaagcgt ggtgcaatgt ctggtgcagg
      541 ctggtttaat tattcctgtc gcgcggctgc gtccggtgct aacgctcaaa aacagtggag
      601 ggaaaaaagg atgatcttgc tacaactctc ctcagcgcag gggccggaag aatgttgtct
      661 cgcagtgaaa aaggcgctgg agcgattaat taaagaagct gcccgacaag acgtcgcggt
      721 aacggtgttg gaaacagaaa cgggccgcta ctctgacacg ctgcgttcag ctctggtttc
      781 tctggatggc gataacgcat gggcattaag cgaaagctgg tgcggcacta ttcagtggat
      841 ttgtccgagt ccgtatcggc ctcatcatgg gcgcaaaaac tggtttctgg gcattgggcg
      901 ttttaccgct gatgagcagg aacaatcgga tgcaatccgt tatgagacgt tgcgttcgtc
      961 ggggccgggc ggtcaacatg tcaataaaac cgactcggcg gtacgcgcca cgcatttggc
     1021 atccggtatt agcgtgaagg ttcagtcaga gcgtagtcag catgctaaca agcggctggc
     1081 acgattgctg attgcctgga agctggagca gcagcaacag gaaaatagcg cggtgctgaa
     1141 atcgcagcgg cgaatgttcc atcaccagat tgaacgtggc aacccgagac ggacatttac
     1201 agggatggct tttatcgaag gataatgaag gatgaaactg ccggaaggcg attaaacgcc
     1261 atccggcagc aaatcaaata attacttcgc cggaatttct ttcagcagtt cagtcagcag
     1321 tgtccagtaa tgacctacgc tttcgatgtg aacttgctca tccggagagt gtggaccggt
     1381 gatagttggc ccgatagaaa ccatgtccat ttccggatac ggttttttga acagaccaca
     1441 ttccaggccc gcgtggataa tctggatgtt cggcgttttg ttgaacagac gctgataggt
     1501 ttcacgtacc agatgcatca ccggagaatt agcgtccggc tgccagccag gatatgcgcc
     1561 tttcgcttcg gttttcgcgc cagccagttt acccagcgaa tccagcatgc tcaccacgta
     1621 gtctttaccg ctgtcgatca gagaacggat cagacagtga atttctacgt tattatcagt
     1681 catggtcacc acaccgacgt tcagggaggt ttcgaccaca ccttttgcca cgtctgagtt
     1741 gcggatcaca ccgttcgggg tggcgttcag cagacgaata aaggtatcgc gagatttcgc
     1801 aatcagggca gctttatcgt tcgctacaga gtccagcaac aaggccagat ttttctcttt
     1861 ctctgccagc tcgtttttca ggatctcctg ataggtattc accagagatt tcaggacgtc
     1921 gactttatca gctgcgacag caatggtcgc aaaggcttca cgcgggatgg cgttacgcag
     1981 tgtgccgccg ttgaaatcga taaggcgcag atccagttct tctgcatgac ccgctaggaa
     2041 gcgcaccagc agtttgttgg cattacccag gccaacgtgg atttccccgc cggagtgacc
     2101 ccctttcaga ccttttaagg ttaacttgaa ggtttcaaaa ccagctggaa ccgcttcacg
     2161 atctaaatgc aggttggagg tgaagtcgat accccccgca caacccatgt agatttcacc
     2221 ttcttcttcg gagtcggtgt taatcagaat atcagcctgc aaccagttgc tctgtaagcc
     2281 gaacgcaccg tccataccgg cttcttcggt catggtcagc agcacttcca gcgggccgtg
     2341 aaccacgttt tcgtcagcca gaaccgccag cgcagacgcc ataccaatgc cgttatctgc
     2401 acccagcgtg gtgccgcgcg ctttaaccca ttcgccatca atataaggct ggataggatc
     2461 tttcgtgaag tcatgcacgg tgtcgttatt tttctgcggc accatatcga ggtgggcctg
     2521 taagacgacc gatttacgat tttccatacc tgcggtagca ggtttacgaa tcaggatatt
     2581 acctacctga tcgcgttcga catggaaacc tttctctttt gcccaaccaa caatgtattc
     2641 agcgagttgc tcttcatgat aggacgggtg aggaatagaa cagattttgg caaaaatatc
     2701 ccacagcggc tgtggagata attgagacag ttcagacacg ttaagtctcc ttgtcgatca
     2761 cccgcaaaac agtattgcag gtcacagggt tagcagaaaa tgttgtcaac acaagacagg
     2821 cttgcgagat atgtttgaga ataccacttt atcccgcgtc agggagaggc agtgcgtaaa
     2881 aagacgcgga ctcatgtgaa atactggttt ttagtgcgcc agatctctat aatctcgcgc
     2941 aacctatttt cccctcgaac actttttaag ccgtagataa acaggctggg acacttcaca
     3001 tgagcgaaaa atacatcgtc acctgggaca tgttgcagat ccatgcacgt aaactcgcaa
     3061 gccgactgat gccttctgaa caatggaaag gcattattgc cgtaagccgt ggcggtctgg
     3121 taccgggtgc gttactggcg cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt
     3181 ccagctacga tcacgacaac cagcgcgagc ttaaagtgct aaaacgcgca gaaggcgatg
     3241 gcgaaggctt catcgttatt gatgacctgg tggataccgg tggtactgcg gttgcgattc
     3301 gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc aaaaccggct ggtcgtccgc
     3361 tggttgatga ctatgttgtt gatatcccgc aagatacctg gattgaacag ccgtgggata
     3421 tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc ctggcactgc
     3481 cgggcgttgt tctttttaac ttcaggcggg ttacaatagt ttccagtaag tattctggag
     3541 gctgcatcca tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat
     3601 cctgaaacct cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg
     3661 gcccttgatg gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct gatgtggatc
     3721 tggcgcggca ttgacccacg tgaaatcctc gacgtccagg cgcgtattgt gatgagcgat
     3781 gccgaacgta ccgacgatga tttatacgat acggtgattg gctaccgtgg tggcaactgg
     3841 atttatgagt gggccatcca ggcgatggtg tggcaacaaa aagcctgtgc ggaagaagat
     3901 ccgcaactca gtggtcgtca ctggctgcat gcggctacgt tgtacaacat tgctgcctat
     3961 cctcatctga aaggagatga cctggccgag caagcgcagg ctttgtcaaa ccgtgcctat
     4021 gaagaagccg cccagcgtct gccgggcacg atgcggcaga tggagtttac cgtacccggc
     4081 ggtgcgccca tcaccggctt tttgcatatg ccgaaaggcg atggcccgtt cccgacagta
     4141 ttaatgtgtg gtggtctgga tgcgatgcag acggactatt acagcctgta tgaacgttat
     4201 tttgcgccgc gcggcattgc gatgctgact attgatatgc cgtcggtggg cttttcatca
     4261 aaatggaagc tcacccagga ctccagcctg ttgcatcagc acgttttaaa ggctctgcct
     4321 aacgtaccgt gggtggatca cactcgcgtc gcggcctttg gtttccgttt cggcgctaac
     4381 gttgccgtgc gtctggcata tcttgaatcg ccgcgtctga aagcggttgc ctgtcttggt
     4441 ccggtagttc ataccctgtt gagtgatttt aagtgccagc aacaggtgcc ggaaatgtat
     4501 cttgacgttc tggcgagtcg tttggggatg catgatgctt ccgacgaagc gttacgcgtg
     4561 gagctgaatc gctattcatt aaaagtgcaa ggattgctgg ggcgtcgctg cccaacgcca
     4621 atgttatcag gctactggaa gaacgatccg ttcagcccgg aagaggactc acgcttaatc
     4681 acctcatcat ctgctgacgg taaattatta gagatcccat ttaacccggt gtatcggaat
     4741 tttgacaaag gtcttcagga aatcaccggc tggatcgaaa aacgcttgtg ttaaaaattt
     4801 gctaaaattt gccaatttgg taaaacagtt gcatcacaac aggagattgc aatgacgtta
     4861 ccgagtggac acccgaagag cagattgatc aaaaaattta ccgcactagg cccgtatatt
     4921 cgtgaaggta agtgcgaaga taatcgattc tttttcgatt gtctggctgt atgcgtcaac
     4981 gtgaaaccgg caccggaagt gcgtgagttc tggggctggt ggatggagct tgaagcgcag
     5041 gaatcccgtt ttacctacag ttaccagttt ggtctgttcg ataaagcagg cgactggaag
     5101 agtgttccgg taaaagacac tgaagtggtt gaacgactgg agcacaccct acgtgagttt
     5161 cacgagaagc tgcgtgaact gctgacgacg ctgaatctga agctggaacc ggcggatgat
     5221 tttcgtgatg aaccggtgaa gttaacggcg tgagtgaaat gtgccggatg tatcacatcc
     5281 ggcaatatcc attaaaactg ataggtcatg ccaaccgcga caatatcatc attattaata
     5341 ttcaatttgt tatcgctatc cagttggttg attttataat caacaaacgc tgacatattt
     5401 ttgttgaaat aatacgtagc accgacgtcg atataattga ccagatcttc atcaccgata
     5461 ccttcaatat ctttcccttt cgataagaca taacccagcg atggacgcag accaaagtca
     5521 aactggtatt gagcgaccgc ttcaaagttc tgtgtcttat tggcaaagcc gccagttatt
     5581 ggcgtcattt tgcgtgtttc agaatagaaa gttgccagat aaatattatt ggcatcgtat
     5641 ttcagacctg ttgcccatgc ttctgcacgc ttgcctgtgc cacggctttg caggttctgc
     5701 tcgttggtgc gatctgagtt ggtataggcc ccactaatgg cgaaatcgct gccgccaaag
     5761 tcatatgtca atgacgtgcc gaagccatcg ccgttttgct ttttaacgtc gcggttttcg
     5821 tttttccctt gatattgcag ggttaagttc aggccatcga taacgccgaa gaagtcggtg
     5881 ttccgatacg tcgccagacc gctggcgcgt ttggtcataa agttgtcggt ctgcgccgag
     5941 gaatcgccac caaattccgg gaacatatcg gtccaggctt ccacgtcata caacgcgccc
     6001 aggttacgac cataatcgaa agaacccaaa tctttatatt tcaacccggc aaaagcgaga
     6061 cgcgttttct gctgtgcagt atcactctcc gctttatttc cggcaaactc cgcttcccac
     6121 cgaccataac cagtcagttg atcgttaatt tgtgtttcac ctttaaaacc aaaacggata
     6181 taactctggt cgccatcttt actggcgtta tcactcatat aatgcatggc tttaactttg
     6241 ccatagacat ccagtttatt accgtcttta ttatatattt ctgcagcctg tacagatgca
     6301 gatgccacaa tgcccatcac cactaatgcc agagtgctct ttttcatttt cattcctgat
     6361 tttaattaac gcgcgaatat tcagcgggag agccccgttg aaaacaggaa agtttttaac
     6421 ctgagattgt taaagatata ttacagatta ataatattct taaaatgtgg taatttatta
     6481 aatctgtaat aaaagcgtaa acaactgccg ctaggcttgc tgatcccgcg caacaaaacg
     6541 ccatgctttg ctcgcagatg gttggcaacc gacgacagtc ctgctaaaac gttcgtttga
     6601 tatcattttt cctaaaattg aatggcagag aatcatgagt gacagccaga cgctggtggt
     6661 aaaactcggc accagtgtgc taacaggcgg atcgcgccgt ctgaaccgtg cccatattgt
     6721 tgaacttgtt cgccagtgcg cgcagttaca tgccgccggg catcggattg ttattgtgac
     6781 gtcgggcgcg atcgccgccg gacgtgagca cctgggttac ccggaactgc cagcgactat
     6841 cgcctcgaaa caactgctgg cagcggtagg gcagagtcga ctgattcaac tgtgggaaca
     6901 gctgttttcg atttatggca ttcacgtcgg gcaaatgctg ctgacccgtg ctgatatgga
     6961 agaccgtgaa cgcttcctga acgcccgcga caccctgcgt gcgttgctcg ataacaatat
     7021 cgttccggta atcaatgaga acgatgctgt cgctacggca gagattaaag tcggcgataa
     7081 cgataacctt tctgcgctgg cggcgattct ggcgggtgcc gataaactgt tgctgctgac
     7141 cgatcaaaaa ggtttgtata ccgctgaccc gcgcagcaat ccgcaggcag aactgattaa
     7201 agatgtttac ggcattgatg acgcactgcg cgcgattgcc ggtgacagcg tttcaggcct
     7261 cggaactggc ggcatgagta ccaaattgca ggccgctgac gtggcttgcc gtgcgggtat
     7321 cgacaccatt attgccgcgg gcagcaagcc gggcgttatt ggtgatgtga tggaaggcat
     7381 ttccgtcggt acgctgttcc atgcccaggc gactccgctt gaaaaccgta aacgctggat
     7441 tttcggtgcg ccgccggcgg gtgaaatcac ggtagatgaa ggggcaactg ccgccattct
     7501 ggaacgcggc agctccctgt tgccgaaagg cattaaaagc gtgactggca atttctcgcg
     7561 tggtgaagtc atccgcattt gcaacctcga aggccgcgat atcgcccacg gcgtcagtcg
     7621 ttacaacagc gatgcattac gccgtattgc cggacaccac tcgcaagaaa ttgatgcaat
     7681 actgggatat gaatacggcc cggttgccgt tcaccgtgat gacatgatta cccgttaagg
     7741 agctggccga tgctggaaca aatgggcatt gctgcgaagc aagcctcgta taaattagcg
     7801 caactctcca gccgcgaaaa aaatcgcgtg ctggaaaaaa tcgccgatga actggaagca
     7861 caaagcgaaa tcatcctcaa cgctaacgcc caggatgttg ctgacgcgcg agccaatggc
     7921 cttagcgaag cgatgcttga ccgtctggca ctgacgcccg cacggctgaa aggcattgcc
     7981 gacgatgtac gtcaggtgtg caacctcgcc gatccggtgg ggcaggtaat cgatggcggc
     8041 gtactggaca gcggcctgcg tcttgagcgt cgtcgcgtac cgctgggggt tattggcgtg
     8101 atttatgaag cgcgcccgaa cgtgacggtt gatgtcgctt cgctgtgcct gaaaaccggt
     8161 aatgcggtga tcctgcgcgg tggcaaagaa acgtgtcgca ctaacgctgc aacggtggcg
     8221 gtgattcagg acgccctgaa atcctgcggc ttaccggcgg gtgccgtgca ggcgattgat
     8281 aatcctgacc gtgcgctggt cagtgaaatg ctgcgtatgg ataaatacat cgacatgctg
     8341 atcccgcgtg gtggcgctgg tttgcataaa ctgtgccgtg aacagtcgac aatcccggtg
     8401 atcacaggtg gtataggcgt atgccatatt tacgttgatg aaagtgtaga gatcgctgaa
     8461 gcattaaaag tgatcgtcaa cgcgaaaact cagcgtccga gcacatgtaa tacggttgaa
     8521 acgttgctgg tgaataaaaa catcgccgat agcttcctgc ccgcattaag caaacaaatg
     8581 gcggaaagcg gcgtgacatt acacgcagat gcagctgcac tggcgcagtt gcaggcaggc
     8641 cctgcgaagg tggttgctgt taaagccgaa gagtatgacg atgagtttct gtcattagat
     8701 ttgaacgtca aaatcgtcag cgatcttgac gatgccatcg cccatattcg tgaacacggc
     8761 acacaacact ccgatgcgat cctgacccgc gatatgcgca acgcccagcg ttttgttaac
     8821 gaagtggatt cgtccgctgt ttacgttaac gcctctacgc gttttaccga cggcggccag
     8881 tttggtctgg gtgcggaagt ggcggtaagc acacaaaaac tccacgcgcg tggcccaatg
     8941 gggctggaag cactgaccac ttacaagtgg atcggcattg gtgattacac cattcgtgcg
     9001 taaataaaac cgggtgatgc aaaagtagcc atttgattca caaggccatt gacgcatcgc
     9061 ccggttagtt ttaaccttgt ccaccgtgat tcacgttcgt gaacatgtcc tttcagggcc
     9121 gatatagctc agttggtaga gcagcgcatt cgtaatgcga aggtcgtagg ttcgactcct
     9181 attatcggca ccattaagaa aattaataac ttagcttaac ttctctaaga aattgtatct
     9241 tctgttatca cttacaaacg cctatacgtt gttatgggct ttggtagtgt aacacgtatg
     9301 ggcgagtagt tcccaggctc aacctcgtaa caagttcctt tgaagctgga agacgtgcca
     9361 ccgtctctaa accttctacc aaaactctta gcaagatgtt ccacacctgg cgcggtgtcc
     9421 aatgatgcta cacgtcgtga gacgttgcag aggataaaca ggctaaatga tgggaagagt
     9481 tttaagtgca atagtggatt aaagcccacc tcttcaaaac ctaacttgat cgggaaagaa
     9541 tcaagctcta gcacggtttc gtccaccgga gtacaacggg tgcctgataa gagaagaact
     9601 gctaagactt catagctttt gtgaaaaagc cctttttagt ggatacgcct acggcgctta
     9661 ttttttggta taaccatcaa aggcgcttta tggctcctat tgtcttgaaa atgtatcttt
     9721 acttgtattg tgttttatgc gtagtctccc tgtgtagtat gtaacttgtt aattttcata
     9781 tgaatacaga ggcacacgat gaaaaacatt gcagcaatca aacgtaataa ccgcaagatt
     9841 cacgctcgta agttcctgtc tacgccagaa ggaaaagcct ggctagagcg taaacagaga
     9901 gagaacgaag aaagaaaact ccttagtgag ttgaaatggc ttagagagga tttctgaaaa
     9961 tctccagaac gcaacaggac gcattctaac gctgttttca ttactcggta tagggatagg
    10021 tgatgtgtgt ttaatgcgtc tgtgcgcttg ttttgtgcct tattttgcat tttcataatg
    10081 cggaagagaa cacggaaaac cttaaaatta attattgggg tatttaaggc tattttcagg
    10141 tgatttaagg gtattaaact tgattaaaat ataaacagat aattatgtat cttattgatt
    10201 tataaggtta tttttaagta aaggcaaata aaaaataaat agccataaat agctataaat
    10261 acatcttaat acctatatcc cttcccttat ggctagattg cgagaggtgc ttaacgcgat
    10321 tttcagcgcc ccccgatctg gaaaatccag aatccccaaa tatcagcaag gaattttctt
    10381 gcgtaatcaa catcgttttt acctattaaa tcattacaca gaaagccttt ctgtaatgtc
    10441 tctcactaaa ctttcactaa acacgagaat gatcggtttt atgtcggatc agatgctaga
    10501 aacagcacca cgtctcacaa gggctgtaag cgatgaaacc agtgtttatg cgggtgcagg
    10561 tcaaaatata ggccaaaatc cacttaacat aataaatgtt atatgtgcta aggaacacat
    10621 ataacgcctt gaattgatgt atcaggggaa aagtgatgga ttttttacgt aggagcacga
    10681 aagggcttct attgactttt gtatgctatg aaatataatt aaagtttttt gtttttattg
    10741 tgtgatgtgg tgggttacac aggggaatag attaaatggt gttctgttta tacaggttta
    10801 tttgttagat caacaatttg gcatttctaa acagatgttt gaacgttgcc aaatgtcttg
    10861 acaacacaag aaaagggctt gctatgccag gcattacacg aaataaggct ataaaaacaa
    10921 aataaatttt aaaatttgca caaacgagaa actcgttcag aaacaagcag aacaattttt
    10981 agccaacaat ttctgatggt gctctgtaag ccgcattaca caaggctttg cgaaagtgat
    11041 gtaaaagtga agaaagtaag aattgaactt ttaagctact tttcattgga ttttttagta
    11101 gggaattaca cagggaatag agagcattat tctatgattt acacagggaa tataggggga
    11161 ttatggcaca cacatagaaa gacattacac agagtgctat gtgacaaatg gatgtaacga
    11221 gaggttacat tcatttatct gaaatgctct ctgtaagaaa gttggtagta acgatatgtt
    11281 acatagacta attattgaat gtaacgatat tacacatcgg tagtaacgat acgttacatc
    11341 ggaagatgat tggatgtaac ggtagctcac agtgaactag gggtaggtca tagtgaacta
    11401 cccccacaga attctgttac ccctatcaag tctgataccc ctatcaaaac tgatacccga
    11461 taagtataaa ta
//
LOCUS       DAZPDU010000138        11428 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZPDU010000138 DAZPDU010000000
VERSION     DAZPDU010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11428
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.140"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(42..383)
                     /gene="bamE"
                     /locus_tag="ACHURS_003287"
     CDS             complement(42..383)
                     /gene="bamE"
                     /locus_tag="ACHURS_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFR1238940.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(532..2193)
                     /gene="recN"
                     /locus_tag="ACHURS_003288"
     CDS             complement(532..2193)
                     /gene="recN"
                     /locus_tag="ACHURS_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFR1238941.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            complement(2279..3157)
                     /gene="nadK"
                     /locus_tag="ACHURS_003289"
     CDS             complement(2279..3157)
                     /gene="nadK"
                     /locus_tag="ACHURS_003289"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFR1238942.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            3280..3873
                     /gene="grpE"
                     /locus_tag="ACHURS_003290"
     CDS             3280..3873
                     /gene="grpE"
                     /locus_tag="ACHURS_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFR1238943.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            complement(3928..5214)
                     /locus_tag="ACHURS_003291"
     CDS             complement(3928..5214)
                     /locus_tag="ACHURS_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFR1238944.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(5235..6026)
                     /locus_tag="ACHURS_003292"
     CDS             complement(5235..6026)
                     /locus_tag="ACHURS_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFR1238945.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            6193..7554
                     /gene="ffh"
                     /locus_tag="ACHURS_003293"
     CDS             6193..7554
                     /gene="ffh"
                     /locus_tag="ACHURS_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFR1238946.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            7803..8051
                     /gene="rpsP"
                     /locus_tag="ACHURS_003294"
     CDS             7803..8051
                     /gene="rpsP"
                     /locus_tag="ACHURS_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFR1238947.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            8070..8618
                     /gene="rimM"
                     /locus_tag="ACHURS_003295"
     CDS             8070..8618
                     /gene="rimM"
                     /locus_tag="ACHURS_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFR1238948.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            8649..9416
                     /gene="trmD"
                     /locus_tag="ACHURS_003296"
     CDS             8649..9416
                     /gene="trmD"
                     /locus_tag="ACHURS_003296"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFR1238949.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            9458..9805
                     /gene="rplS"
                     /locus_tag="ACHURS_003297"
     CDS             9458..9805
                     /gene="rplS"
                     /locus_tag="ACHURS_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFR1238950.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(9882..10364)
                     /locus_tag="ACHURS_003298"
     CDS             complement(9882..10364)
                     /locus_tag="ACHURS_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFR1238951.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(10380..>11428)
                     /gene="dgcN"
                     /locus_tag="ACHURS_003299"
     CDS             complement(10380..>11428)
                     /gene="dgcN"
                     /locus_tag="ACHURS_003299"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFR1238952.1"
                     /translation="TYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNILAS
                     WHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSLAV
                     LTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFHRF
                     ALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDARK
                     TSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEVGGLRHKAYRLGGDEFAMVLY
                     DVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELADHN
                     MYQAKHQRAEKLVR"
BASE COUNT         2804 a         2748 c         3060 g         2816 t
ORIGIN      
        1 attgagcacc ttttttaacg tctttgagag caactttatt attagttacc actcagcgca
       61 ggtttgttat caatattggt caacacaccg ctactgttaa aggtcagcgt cagcgtttgc
      121 tgcgttacac cttcatgacc tggttgctgg cggaagacat agaaccaggt attcgtacca
      181 aatggatcgg acatcagcgg tgtacccaat gcgtacgcaa cttgttgttg cgtcatgcca
      241 acacgtattt tggatacgtc gttagcggtc agatagttcc cctggttgat gtcaggacgg
      301 taaaccactc gctccagagt ggaacagcct gcggtcaaca tcaatagtac tgctgctgca
      361 gcagtcagcg ttttacagcg catagtgatt tgattccttt tcgggcccga gcagtacgtg
      421 gctcatctgt aatatgccga tgataataga cctttcatca ctttaaaacc ttttgctttc
      481 cggtcttacg gcgttttgct gtttactcta accgtgaagc aggaaaaaag tttacgctgc
      541 aagcagttct ttcgcattcg ctagtgtatt acgtgtgact tcactgccac caagcaggcg
      601 cgccagctct tgtaaccgcg cttttttatt cagggattgc atatgcgttt ctgtcatcgc
      661 accatcggtt tctttgctga caaaatagtg ttgatgacca catcccgcga cttgtggcag
      721 gtgggtaaca cacatcacct gagttgattc gccgagttga cgcagcagtt tgccgacaac
      781 tgccgctgtt ggaccgctaa tccctacatc cacttcatca aaaatcagtg ccggggtttc
      841 cattttacgc gccgtgatga cctggattgc cagtgcgatg cgggacaatt caccaccgga
      901 tgcgactttg gcaataggct gcattggctg acctgggttg gtggttaccc gaaactcaat
      961 acgatcggca ccgtcagcgc ccaggtgatg ctcgtcaaat ttaacatcga tcgtaaactg
     1021 cccatgcggc attgagagcg catgcatact gtcggtgatc agctgtgcaa gttcttctgc
     1081 atattgctgg cgttgttggt gtaatgcgcg cgcgatttcc agtgcctgct gatgatgttt
     1141 cgttaccgcc agcgcaagcg tttcttgtga gtcggcctga tcgtccagtt gctgctgttc
     1201 ttccagtagc gactggtaat actgtggcaa tgcctcaggg ctgacgtgat gtttacgtgc
     1261 cagcgaaatc tgttttgaga tgcgctgttc aagttcaaat agtcggttgg gatcgagatc
     1321 cagacgatcg cagtagtggc gcagttcatc gctggcttca gcaatctgga tggtagcttc
     1381 ttccagcata tcaagtacgc cggacagttt gctgtccatg ccaatcaatt cgctcaccag
     1441 ttgtttagcc gtgtaaagct gactttgcag gtttgcgtct tcaccgtcgg ccattaatgc
     1501 caatgcattc tggctggtgg tcagcaattg accgctgttc gccagacgtt tgtactcttc
     1561 gtcgatttgt tcaaactctc cgggctgcgg attaaattcg ttaagttctt ttaattggta
     1621 ttgcagcagt tccgcacggg cggcgcgttc ctgacttaac tgttgatgat gcgcgaggtc
     1681 acggcagctt tgatgccaca actgataacg tgcggtcatt tcctgcagta gagaggtttc
     1741 attggcatag ccatcaagca ggaatttttg gtgctcaggt ttggtgagta attgatgagc
     1801 gtgctgacca tggatctgaa tcagcaactg acccagttcg cgcagttgtg acagaggaac
     1861 agctgtaccg ttgatgaaac cacgggagcg accatcgctg ctgatcacgc gacgaagcaa
     1921 acattcatgc ccgtcttcaa gctggttttc ttccagccag cgcagagccg ctggcgtatc
     1981 tttcagagaa aaacgggcgc acaggtcagc gcgagcagcg ccggtacgca ccatgtcggc
     2041 ttcagcgcga ccaccgagac aaagaccgag ggcatctatt gcaatagatt tacccgcgcc
     2101 ggtctcgcca gttattacgg tcatgccgct atgaaaatca atctcaagct cacgaacgat
     2161 agcaaagttg ctgatggtca gttgtgccaa catagtcgtt ttcctgtatg aaaaaccatt
     2221 actgttattg tgtacagtat aaactggttt tatatacagt aaagaggctg gcgtaaaatt
     2281 agaataattt ttttgaccag ccgagcttgg tgcttaatgt gttgaaataa ctgtaatctt
     2341 tcggatgaat cagattcaga tggtaatcac agcgacgaat caggacatct tcaccttcct
     2401 gaatcggcag tgctatctgg ctgtcgcaac tgatttccag gtcgttacgg cgatgcgaaa
     2461 aacgcagacg gatcgtgctg ctgctgttta tgaccagtgg tcgtgctgac aacgtatgcg
     2521 ggaacatggg caccagggta atcgcatcca gagagggggt cagaatagga ccgcctgcag
     2581 agagggaata ggcggtggag cctgttggcg tcgaaataat tagtccatca gatcgctgag
     2641 aaaacgcaaa gatctcgtcg atatacactt cgaactcaat catatgcgcc actttgcctg
     2701 gatgaagcac cacttcattt atcgcggtgc tgatgcgttt ctggcaatct tgctgacaga
     2761 cttgcgcttc cagcaaaaaa cgtttctcgc tgatgtagtg gccttccagc acatcggcta
     2821 actgttgctg ggcgttatcg gggtcaaggt cagtcaggaa acccaggttg ccacggttga
     2881 ttccaataac tttaatatcg taacgggcga gtgtgcgtgc cgcgcccagc atattaccgt
     2941 cgccaccaac gactaccgcg agatcagcta gttgcccaat ctccgcgagc gtgccagttt
     3001 tcacattctt cagttgcagt tcgtgagcga tttgttgctc aacgatgacc tcgtaacctt
     3061 ttgtgcacag ccagcggtag agcatttcat gtgttgtcag tgcagtgggg tgccgtgggt
     3121 gtcccacaat gccaatacac ttgaaatgat tattcatttt tccgaggtcc ttgttgcgaa
     3181 gattgatgac aatgtgagtg cttcccttga aaccctgaaa ctgatcccca taataagcga
     3241 agttagcgag atgaatgcga aaaaaacgcg gagaaattca tgagtagtaa agaacagaaa
     3301 acgcctgagg ggcaagcccc ggaagaaatt atcatggatc agcacgaaga gattgaggca
     3361 gttgagccag aagcttctgc tgagcaggtg gatccgcgcg atgaaaaaat tgcgaatctc
     3421 gaagctcagc tggctgaagc ccagacccgt gaacgtgacg gcattttgcg tgtaaaagcc
     3481 gaaatggaaa acctgcgtcg acgtactgaa ctggatattg aaaaagccca caaattcgcg
     3541 ctggagaaat tcatcaacga attgctgccg gtgattgata gcctggatcg tgcgctggaa
     3601 gtggctgata aagctaaccc ggatatgtct gcgatggttg aaggcattga gctgacgctg
     3661 aagtcgatgc tggatgttgt gcgtaagttt ggtgttgaag tgatcgccga aactaacgtg
     3721 ccgctggacc cgaatgtgca tcaggctatc gcaatggtgg aatctgatga cgttgcgcca
     3781 ggtaacgtac tgggcattat gcagaagggt tatacgctga atggtcgtac gattcgtgcg
     3841 gcgatggtaa ctgtagcgaa agcaaaagcg taatttctgt tttcgtaata attcacggcc
     3901 ctgcatgcga atgcagggcc gttttcgtta ctccgccaca ctctcgcgca gcggtttcac
     3961 aggaaaaact tttacctgct taatcatatt gtcctgtacg tcgagaatat ctatatcgta
     4021 ctcgccaata cgcacgcggg tgcctgcgac agggatctct tccagtgcct caagaatgac
     4081 gccattaacc gtgcgggcat catcttccgg tagatgccaa ttaaaggctt tgttgatttc
     4141 ccgcacgttg gcggtgccat cgataatcac cgaaccgtcg ttttgcggcg tgacctcttc
     4201 ggcaagtgtt ggcgacatcg acgtagtgaa atcgccgaca atctcttcca gaatatcttc
     4261 aaccgtcacc agcccctgaa tgtctccata ctcgttgacg accaggccga ctttcttttt
     4321 gttgcgctga aactttacca actgcgtgct gagcggcgta ccttccggca caaaatagat
     4381 ctcgtccgcg gcgcgcagca tggtttcttt ggtgaactct tttttctccg acatcaaccg
     4441 ccaggcttca cgtactcgca gcatactgat ggcgtcgtcc agcgaatcac ggtagagcac
     4501 gatgcgcccg tgaggtgagt gggagagttg gcgcagaatc gatttccagt catcgttgat
     4561 atcaataccg ataatttcac tgcgcggcac catgatgtca tcaacggtca ttttttccag
     4621 atcgagcacc gacagcagca tatcctgatt gcgacgggaa atttgtgagc gtgattcgtg
     4681 cacgatagtg cgcaactctt ctttgctcaa agagccgcta accacgatat cggttttgat
     4741 acccatcatg cgcatcagca tacgggtgat agcattcagc aaccagacca gcggcatcat
     4801 caaaatttgc agcggagcca gcagaaaact actcggataa gcgacttttt ccgggtacag
     4861 cgcggcaatg gttttcggca atacctcagc aaataccagt acgacaaaag tcagcacacc
     4921 agtcgcaatt gccacgcccg catcgccgta caaacgcatc ccaacaatag tgccgagcgc
     4981 ggaggcaaga atattgacca ggttattgcc gattaacacc aggcttatca ggcggtctgg
     5041 cttacgcagc aatttttcga cgcgtttggc cgagcgatta ccctgtttcg ccatatgtcg
     5101 cagacgatag cggttgaggg tcatcattcc ggtttcggac ccggaaaaat aggctgaaat
     5161 gaccaccatg atgatcagaa taatgatcaa cgtagtagta gaaatgtgtt ccaggggaaa
     5221 ctccttttct gggtttagct gattaactgc tggacaattc ggctgccgaa gtaggccagt
     5281 gtcagaatta ccgcgcccgc aacgttaaac cagacgacgc ggcgtccacg ccatccttca
     5341 tggtaatgtc cccacagcag cacaatatag acaaaccacg ccacgataga gagcacagcc
     5401 ttgtcgatat tttccatgct aaataggttg tgcatgtaga acaggccagt gcaaagcgtg
     5461 agcgttagca gcaccacgcc aatctgcgtg atgtggaaca ttttacgctc gatactcatc
     5521 aatggcggca tttcctggtt aaatgccagc ttcttgttct tcagttggta atcaatccac
     5581 gccagttgca gcgcgtacag ggcggcgata attagcgtgg cataggaaaa gagcgataag
     5641 ccaatgtgca ccagcatccc aggcgttgct tccagatggg tgatgtattc attgggcatg
     5701 aaggtcgcca gcgccaggtt gataagcgca aaggcataga caatgggtag cagcaaccag
     5761 ccacgattgc gagaagccac aatggtcatt accgtacaga tcatcaaact gaccaatgaa
     5821 ccaacgttca gcaggctgag gttttgtccg ctatcaccgt cgggcaggat gcgggcttcc
     5881 agagcgattg cgtggcagac cagcgcaatg accgcagaaa taatagccat gcgccgccag
     5941 ccgccgtttt tttgcagcag accgggaaca atcagcgcaa gactgacgga gtaggcgaca
     6001 agcgcgagca gagcaaaaac gggcatagtg atgtcgacag tttgagcaat aagaaagaga
     6061 agcagtatag cgtcaggtga acgctgctcc aaccgttgca taacaacaaa gacgccttca
     6121 tgttatactg cggcaaaata ctgatgatgt gtcgcgattg cggccaaccg tttccacccc
     6181 aggcgagaga caatgtttga taatttaacc gatcgtttgt cgcgcacgct gcgcaatatc
     6241 agtggccgtg gacgcctcac tgaagacaac gtaaaagata cgctgcgcga agtgcgcatg
     6301 gcgctgctgg aggcggatgt cgctctgccg gtagtgcgtg agtttatcaa tcgcgtaaaa
     6361 gagaaagcgg ttggtcatga agttaataag agcctgacgc cggggcagga gttcgtcaaa
     6421 atcgtccgta acgaactggt tgcggcgatg ggcgaagaga accagaccct gaacctggcg
     6481 gcgcaaccgc ctgcggtcgt gctgatggcg ggcctgcaag gtgccggtaa aacaaccagc
     6541 gttggtaaac tcggtaagtt cctgcgcgag aagcacaaga agaaagtgct ggtggtttct
     6601 gccgacgttt atcgcccggc ggcaatcaaa cagcttgaga cgctggcaga gcaggtgggc
     6661 gttgatttct tcccttctga tgttggtcag aagccggtag atatcgttaa cgcggcgctg
     6721 aaagaagcca aactgaaatt ctacgacgtg ctgctggtgg ataccgctgg tcgtctgcac
     6781 gttgacgaag cgatgatgga cgagatcaaa caagtccatg cgtcgattaa cccggttgaa
     6841 accctgtttg tggttgacgc catgaccggt caggatgcgg ccaatacggc aaaagcattc
     6901 aatgaagcgt taccgcttac cggcgtagtg ttgaccaaag tggacggcga tgcccgcggc
     6961 ggtgcggcgc tctctattcg tcacatcact ggcaaaccga tcaagtttct cggtgttggc
     7021 gagaaaactg aggcgctgga gccgttccat ccggaccgca tcgcgtcgcg tattctcggc
     7081 atgggcgacg tactgtcgct gatcgaagat atcgaaagca aagttgaccg cgcgcaggca
     7141 gagaaattag ccagcaagct gaaaaaaggc gacggcttcg atctcaacga ttttcttgag
     7201 cagctgcgcc agatgaaaaa tatgggcggc atggctagcc tgatgggcaa gctgccgggc
     7261 atggggcaga tcccggataa tgttaagtcg cagatggacg ataaagtgct ggtgcgtatg
     7321 gaagccatca tcaactcgat gacgatgaaa gagcgcgcta agccagaaat tatcaaaggt
     7381 tcgcgtaagc gtcgtatcgc tgccggttgc ggtatgcagg tgcaggacgt taaccgcctt
     7441 ctgaagcagt tcgacgacat gcagcgcatg atgaagaaaa tgaagaaggg cggaatggcg
     7501 aagatgatga gaagcatgaa gggtatgatg cccccaggct tccctggtcg ctaatcggcc
     7561 tggcttgttt gccattttgg caccgggatg tgctcgtcat cctcacgtac tgcgtgtacg
     7621 ctccggtgac tgcgcgcact ccggcaccaa actggctgca ccgccgacgg ccttttacag
     7681 cagagttagc aactgttgat tgcaattccc gcaaaaatga gtaaaatttt cgggctttta
     7741 atatgacacc ggactccgtt cctcgatggg gtccggttgt tttattcaca caagaggatg
     7801 ttatggtaac tattcgttta gcacgtcacg gcgctaaaaa gcgtccgttc taccaggttg
     7861 ttgtcgctga cagccgtaat gcacgcaacg gtcgcttcat cgagcgcgtt ggtttcttca
     7921 acccaatcgc tagcgaaaaa gaagaaggca ctcgcctgga tctggatcgc atcgctcact
     7981 gggttggcca gggcgcaact atttctgatc gcgttgctgc gctgatcaaa gaagtaaaca
     8041 aagcagctta atctgtcacg gtggtcatga tgagcaaaca actcaccgcg caagcacctg
     8101 ttgatcccat cgttttggga aaaatgggtt cgtcttacgg tattcgtggg tggctcagag
     8161 tgttttcttc caccgaagac gccgaaagca tttttgacta tcagccctgg tttatccaga
     8221 aggcgggtca gtggcagcaa gtccagctgg aaagctggaa gcaccacaat caggacatga
     8281 tcatcaagct gaaaggcgtt gacgatcgtg atgcggcgaa cctgctgacg aattgtgaaa
     8341 ttgtcgtgga ttcatcgcag ctgcctcagc ttgaagaggg ggactactac tggaaagacc
     8401 tgatgggctg ccaggtagta accactgaag gctacgatct cggtaaagtc gtcgatatga
     8461 tggaaaccgg atctaatgac gttctcgtca ttaaggcaaa cctgaaagat gcgtttggta
     8521 tcaaggaacg tctcgtaccg ttcctcgatg ggcaggttat caagaaagtc gatctcacta
     8581 ctcgttcaat cgaagtagat tgggatcctg gtttttaaac caccggataa acggtaaaag
     8641 acggcgctat gtggattggc ataattagcc tgtttcctga aatgttccgc gcaattaccg
     8701 attacggggt aactggccgg gcagttaaaa atggcctgct gagcatccag agttggagtc
     8761 ctcgcgactt cacgcatgac cggcaccgta ccgtggacga tcgtccttac ggcggcggac
     8821 cggggatgtt aatgatggtg caacccttgc gggacgccat tcatgcagca aaagccgcgg
     8881 cgggtgaagg cgcaaaggtg atttatctgt caccacaggg acgcaagctt gatcaagcgg
     8941 gcgtcagcga actggcaacg aatcaaaaat tgattctggt gtgcggtcgc tacgaaggta
     9001 tagatgagcg cgtgatccaa accgaaattg acgaagaatg gtcaatcggc gattacgttc
     9061 tcagtggtgg tgagttacca gcaatgacgc tgattgactc cgtttcccgg tttattccgg
     9121 gagtactggg acatgaagcc tcggcaacgg aagattcctt tgctgaagga ttgctggatt
     9181 gcccgcacta tacgcggcct gaggtgttag aagggatgga agttccgcca gtgttactgt
     9241 cgggcaacca tgccgagata cgtcgctggc gtttgaaaca gtcgctgggc cgtacctggc
     9301 ttagaagacc tgaacttctg gaaaacctgg ctctgactga agagcaagca aggttgctgg
     9361 cggagttcaa aacggaacac gcacaacagc aacataaaca tgatgggatg gcgtaagccc
     9421 ccgagatatc agtttaccca ggataagaga ttaaattatg agcaatatta ttaagcaact
     9481 tgaacaagag cagatgaagc aggacgtacc ttccttccgt ccgggtgata ccgtggaagt
     9541 gaaagtatgg gttgttgaag gttccaaaaa acgtctgcag gcattcgagg gcgtggttat
     9601 cgctattcgt aaccgcggtc tgcactctgc attcactgtt cgtaaaattt ccaacggcga
     9661 aggcgttgag cgtgtcttcc agactcactc tccggtagtt gacagcattt ctgtcaaacg
     9721 tcgtggtgct gttcgtaaag ctaaactgta ctacctgcgt gagcgtactg gtaaggctgc
     9781 tcgtatcaaa gagcgtctta actaagattt cgcttaagcg acatcctgtt aagaagggct
     9841 ggccaattgg ctggcccttt ttttgtctgt ttgctcgtgt tttaaggggt tgtaatcacc
     9901 actgcgacgc ggcggttctc ggcgcggccc tgggcggtct tgttactggc tatgggatat
     9961 ttttttccta aaccctgtgt ggtgagattg ctgcgtggaa tttgtccacc aatagcccat
    10021 gcatcggcca cgacattcgc ccgtttcaat gataagcctt cgttgtaact gtcttcacca
    10081 tagttatcgg tgtgtccatc catacgggca tgtgttagcc ctgtcgaggc caatttggct
    10141 gccatggttt ggatctgttg ctggctttcc gggagcaatt tgtagtcatt ttttgcgaac
    10201 agaatggcat ctgataagcc cagcgaccag tcgccggcgg attcagtaaa tccataagat
    10261 tgcatagcgg cgacttgctc aggagtaaac tttccctgag gggactggca gccagtcagt
    10321 attagtgagg tgaaaatcag gggtgctacc aggtgcttta tcatatcgat atatccttgt
    10381 tatctcacca gcttttcggc acgctggtgt ttggcctgat acatattgtg atcggcaagc
    10441 tcttgtaatt tttccgcaga ggcgtgctca atggtcatcg cgtaaccaat gcttaatgtc
    10501 atggtggtct gatggccatt atgaagatca aacgggagat taaagatttg tgtcagtgct
    10561 gagcatatct gctgcacttc agattctgac tgtacatcat agagcaccat agcgaattca
    10621 tcgccgccca ggcggtatgc tttatgtcgc agcccgccaa cttcagctaa ccgttttgcg
    10681 atttcaatca agactctatc gcccgtcgca tgaccccagg tatcattgat gtatttgaaa
    10741 ttatcgccat caagaaatag taacgccgac gtttttcggg catcggaatt gttcatcaac
    10801 gtgttgatgc cgctacgaaa cgctgcgcgg ttagccagcc cggttaatgg gtcatgtagc
    10861 gcggtacgta gaagctgcgc atttttagcc tgtaaacgaa gctgccactc ttccatttca
    10921 tccagcagac tgttgaagtc gagagcgaag cggtgaaact cagcgatacg ttcttccgaa
    10981 actcgtcggg aaaaattgcg gttggaacgc acatcatgta cgacatcggt gatatttttc
    11041 agtgcttcca ccaggccatt gtgcaaatgg cgggtgaggg taattgcgat gcctgatgcc
    11101 agcagaatac aaccggtcag tacggcgagc gaaaaccaga taaaatggct gattgaactg
    11161 tcgcgagcgg ttaagcgtac ttcgccaatg gtttcaccat tgtgacgaat cggctgaatg
    11221 atgggggcgg ggaagagcca gtggcttatg aaattgctga aagtatcgcc tggatcctta
    11281 cgggtgtaat gccaggacgc cagaatattt tgctgcttat cacgtacttc tgcagttgaa
    11341 aattgccctt gctggcccag cgctgccagt gtttcagttg ctgcagggcc atcggcaaaa
    11401 acgacagctg cttccagact gtaagtca
//
LOCUS       DAZPDU010000139        11295 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZPDU010000139 DAZPDU010000000
VERSION     DAZPDU010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11295
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.141"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..741
                     /gene="hdhA"
                     /locus_tag="ACHURS_003300"
     CDS             <1..741
                     /gene="hdhA"
                     /locus_tag="ACHURS_003300"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFR1238953.1"
                     /translation="DGKCAIITGAGAGIGKEIAITFATAGASVVVSDINADAANHVVD
                     EIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPKPFDMPMADF
                     RRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYASSKAAASHL
                     VRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRLGQPQDIANA
                     ALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            962..1552
                     /gene="uidR"
                     /locus_tag="ACHURS_003301"
     CDS             962..1552
                     /gene="uidR"
                     /locus_tag="ACHURS_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFR1238954.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIKGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1941..3752
                     /gene="uidA"
                     /locus_tag="ACHURS_003302"
     CDS             1941..3752
                     /gene="uidA"
                     /locus_tag="ACHURS_003302"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFR1238955.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            3749..5122
                     /gene="uidB"
                     /locus_tag="ACHURS_003303"
     CDS             3749..5122
                     /gene="uidB"
                     /locus_tag="ACHURS_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFR1238956.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGVLFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSRKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            5161..6471
                     /gene="uidC"
                     /locus_tag="ACHURS_003304"
     CDS             5161..6471
                     /gene="uidC"
                     /locus_tag="ACHURS_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFR1238957.1"
                     /translation="MRKIVAMAVICLTAASGLISAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKSGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAQIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLKSEITPERKTSGVSDC"
     gene            complement(6652..8160)
                     /locus_tag="ACHURS_003305"
     CDS             complement(6652..8160)
                     /locus_tag="ACHURS_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFR1238958.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(8261..9436)
                     /gene="manA"
                     /locus_tag="ACHURS_003306"
     CDS             complement(8261..9436)
                     /gene="manA"
                     /locus_tag="ACHURS_003306"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFR1238959.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            9635..11281
                     /gene="fumB"
                     /locus_tag="ACHURS_003307"
     CDS             9635..11281
                     /gene="fumB"
                     /locus_tag="ACHURS_003307"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFR1238960.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
BASE COUNT         2812 a         2650 c         2916 g         2917 t
ORIGIN      
        1 gacggaaaat gcgccatcat cacaggtgcg ggtgcaggta ttggtaaaga aatcgccatt
       61 acattcgcga cagctggcgc atctgtggtg gtcagtgata ttaacgccga cgcagctaac
      121 catgttgtag acgaaattca acaactgggt ggtcaggcat ttgcctgccg ttgtgatatt
      181 acttccgaac aggaactctc tgcactggca gactttgcta tcagtaagct gggtaaagtt
      241 gatattctgg ttaacaacgc cggtggcggt ggacctaaac cgtttgatat gccaatggcg
      301 gattttcgcc gtgcttatga actgaatgtg ttttcttttt tccatctgtc acaacttgtt
      361 gcgccagaaa tggaaaaaaa tggcggtggc gttattctga ccatcacttc tatggcggca
      421 gaaaataaaa atataaacat gacttcctat gcatcatcta aagctgcagc cagtcatctg
      481 gtcagaaata tggcgtttga cctgggtgaa aaaaatattc gggtaaatgg cattgcgccg
      541 ggggcaatat taaccgatgc cctgaaatcc gttattacac cagaaattga acaaaaaatg
      601 ttacagcaca cgccgatcag acgtctgggc caaccgcaag atattgctaa cgcagcgctg
      661 ttcctttgct cgcctgctgc gagctgggta agcggacaaa ttctcaccgt ctccggtggt
      721 ggggtacagg agctcaatta atacactaac ggaccggtaa acaaccgtgc gtgttgttta
      781 ccgggataaa ctcatcaacg tctctgctaa ataactggca gccaaatcac ggctattggt
      841 taaccaattt cagagtgaaa agtatacgaa tagagtgtgc cttcgcacta ttcaacagca
      901 atgataggcg ctcacctgac aacgcggtaa actagttatt cacgctaact ataatggttt
      961 aatgatggat aacatgcaga ctgaagcaca accgacacgg acccggatcc tcaatgctgc
     1021 cagagagatt ttttcagaaa atggatttca cagtgcctcg atgaaagcca tctgtaaatc
     1081 ttgcgccatt agtcccggga cgctctatca ccatttcatc tccaaagaag ccttgattca
     1141 ggcgattatc ttacaggacc aggagagggc gctggctcgt ttccgggaac cgattaaagg
     1201 gattcatttt gttgactata tggtcgagtc tattgtctct ctcacccatg aagcctttgg
     1261 gcaacgggcg ctggtggtcg aaattatggc ggaagggatg cgtaacccac aggtcgccgc
     1321 catgcttaaa aataagcata tgacgatcac ggaatttgtt gcccagcgga tgcgtgatgc
     1381 ccagcaaaaa ggcgagataa gcccagacat caacacgtca atgacttcac gtttactgct
     1441 ggatctgacc tacggtgtac tggccgatat cgaagcggaa gatctggcgc gtgaagcgtc
     1501 gtttgctcag ggattacgcg cgatgattgg cggtatctta accgcatcct gatcctctca
     1561 tttcgcgggc tggtgataac tgtgcccgcg tttcatactt taatttccct gtacaaaata
     1621 caatctagcc gggatcggag aattcccccc caaaaatatt caccgtagcc atatgtcatg
     1681 agagtttatc gttcccaata tgctcgaacg aacgttcggt tgcttatttt atggcttctg
     1741 tcaacgccgt tttaaagatt aatgcgatca atatcacgct gtgggtattg cagtttttgg
     1801 ttttttgatc gcggtgtcag ttctttttat ttccatttct cttccatggg tttctcacag
     1861 ataactgtgt gcaacacaga attggttaac taatcagatt aaaggttgac cagtattttt
     1921 atcttaatga ggagtccctt atgttacgtc ctgtagaaac cccaacccgt gaaatcaaaa
     1981 aactcgacgg cctgtgggca ttcagtctgg atcgcgaaaa ctgtggaatt gatcagcgtt
     2041 ggtgggaaag cgcgttacaa gaaagccggg caattgctgt gccaggcagt tttaacgatc
     2101 agttcgccga tgcagatatt cgtaattatg cgggcaacgt ctggtatcag cgcgaagtct
     2161 ttataccgaa aggttgggca ggccagcgta tcgtgctgcg tttcgatgcg gtcactcatt
     2221 acggcaaagt gtgggtcaat aatcaggaag tgatggagca tcagggcggc tatacgccat
     2281 ttgaagccga tgtcacgccg tatgttattg ccgggaaaag tgtacgtatc accgtttgtg
     2341 tgaacaacga actgaactgg cagactatcc cgccgggaat ggtgattacc gacgaaaacg
     2401 gcaagaaaaa gcagtcttac ttccatgatt tctttaacta tgccgggatc catcgcagcg
     2461 taatgctcta caccacgccg aacacctggg tggacgatat caccgtggtg acgcatgtcg
     2521 cgcaagactg taaccacgcg tctgttgact ggcaggtggt ggccaatggt gatgtcagcg
     2581 ttgaactgcg tgatgcggat caacaggtgg ttgcaactgg acaaggcact agcgggactt
     2641 tgcaagtggt gaatccgcac ctctggcaac cgggtgaagg ttatctctat gaactgtgcg
     2701 tcacagccaa aagccagaca gagtgtgata tctacccgct tcgcgtcggc atccggtcag
     2761 tggcagtgaa gggcgaacag ttcctgatta accacaaacc gttctacttt actggctttg
     2821 gtcgtcatga agatgcggac ttgcgtggca aaggattcga taacgtgctg atggtgcacg
     2881 accacgcatt aatggactgg attggggcca actcctaccg tacctcgcat tacccttacg
     2941 ctgaagagat gctcgactgg gcagatgaac atggcatcgt ggtgattgat gaaactgctg
     3001 ctgtcggctt taacctctct ttaggcattg gtttcgaagc gggcaacaag ccgaaagaac
     3061 tgtacagcga agaggcagtc aacggggaaa ctcagcaagc gcacttacag gcgattaaag
     3121 agctgatagc gcgtgacaaa aaccacccaa gcgtggtgat gtggagtatt gccaacgaac
     3181 cggatacccg tccgcaaggt gcacgggaat atttcgcgcc actggcggaa gcaacgcgta
     3241 aactcgaccc gacgcgtccg atcacctgcg tcaatgtaat gttctgcgac gctcacaccg
     3301 ataccatcag cgatctcttt gatgtgctgt gcctgaaccg ttattacgga tggtatgtcc
     3361 aaagcggcga tttggaaacg gcagagaagg tactggaaaa agaacttctg gcctggcagg
     3421 agaaactgca tcagccgatt atcatcaccg aatacggcgt ggatacgtta gccgggctgc
     3481 actcaatgta caccgacatg tggagtgaag agtatcagtg tgcatggctg gatatgtatc
     3541 accgcgtctt tgatcgcgtc agcgccgtcg tcggtgaaca ggtatggaat ttcgccgatt
     3601 ttgcgacctc gcaaggcata ttgcgcgttg gcggtaacaa gaaggggatc ttcacccgcg
     3661 accgcaaacc gaagtcggcg gcttttctgc tgcaaaaacg ctggactggc atgaacttcg
     3721 gtgaaaaacc gcagcaggga ggcaaacaat gaatcaacaa ctctcctggc gcaccatcgt
     3781 cggctacagc ctcggtgacg tcgccaataa cttcgccttc gcaatggggg tgctcttcct
     3841 gttgagttac tacaccgacg tcgctggcgt cggtgccgct gcggcgggca ccatgctgtt
     3901 actggtgcgg gtattcgatg ccttcgccga cgtctttgcc ggacgagtgg tggacagtgt
     3961 gaatacccgc tggggaaaat tccgcccgtt tttactcttc ggtactgcgc cgttaatgat
     4021 cttcagcgtg ctggtattct gggtaccgac cgactggagc catagccgca aagtagtgta
     4081 tgcatatttg acctacatgg gcctcgggct ttgctacagc ctggtgaata ttccttatgg
     4141 ttcacttgct accgcgatga cccaacaacc acaatcccgc gcccgtctgg gcgcggctcg
     4201 tgggattgcc gcttcattga cctttgtctg cctggcattt ctgatagggc cgagcattaa
     4261 gaactccagc ccggaagaga tggtgtcggt ataccatttc tggacgattg tgctggcgat
     4321 tgccggaatg gtgctttact tcatctgctt caaatcgacg cgtgagaatg tggtacgtat
     4381 cgttgcgcag ccgtcattga agatcagttt gcaaaccctg aaacggaatc gcccgctgtt
     4441 tatgttgtgc atcggtgcgc tgtgtgtgct gatttcgacc tttgcggtca gcgcctcgtc
     4501 gttgttctac gtgcgctatg tgttaaatga taccgggctg ttcactgtgc tggtactggt
     4561 gcaaaacctg gttggtactg tggcatcggc accgcttgtg ccggggatgg tcgcgaggat
     4621 cggtaaaaag aataccttcc tgattggcgc tttgctggga acctgcggtt atctgctatt
     4681 cttctgggtt tccgtctggt cactgccggt ggcgttggtt gcgttggcca tcgcttcaat
     4741 tggtcagggc gttactatga cagtgatgtg ggcactggaa gctgataccg tagaatacgg
     4801 tgaatacctg accggcgtgc gaattgaagg gctcacctat tcactattct catttacccg
     4861 taaatgcggt caggcaatcg gaggttcaat tcctgccttt attttggggt taagcggata
     4921 tattgccaat caggtgcaaa cgccggaagt tattatgggc atccgcacat caattgcctt
     4981 agtaccttgc ggatttatgc tactggcatt cgttattatc tggttttatc cgcttacgga
     5041 taaaaaattc aaagaaatcg tggttgaaat tgataatcgt aaaaaagtgc agcagcaatt
     5101 aatcagcgat atcactaatt aataatcaat aaaaataatc agaacatcaa aggtgcaact
     5161 atgagaaaaa tagtggccat ggccgttatt tgcctgacgg ctgcctctgg ccttatctct
     5221 gcttatgcgg cgcaactggc tgacgatgag gcgggactac gcatcagact gaaaaacgaa
     5281 ttgcgcaggg cggataagcc cagtgctggc gcgggaagag atatttacgc atgggtacag
     5341 ggaggattgc tcgatttcaa tagtggttat tattccaata ttattggcgt tgaaggcggg
     5401 gcatattatg tttataaatt aggtgctcgt gctgatatga gtacccggtg gtatcttgat
     5461 ggtgataaaa gttttggctt tgccctaggg gcagtaaaaa taaaacccag tgaaaatagc
     5521 ctgcttaaat caggtcgctt cgggacggat tatagttatg gtagcttacc ttatcgtatt
     5581 ccgttaatgg ctggcagttc gcaacgtaca ttaccgacag tttctgaagg agcattaggt
     5641 tattgggctt taacaccaaa tattgatctg tggggaatgt ggcgttcacg agtattttta
     5701 tggactgatt caacaaccgg tattcgtgat gaaggggtgt ataacagcca gacgggaaaa
     5761 tacgataaac atcgcgcacg ttctttttta gccgccagtt ggcatgatga taccagtcgc
     5821 tattctctgg gggcatcggt acagaaagat gtttccaatc agatacaaag tattctcgag
     5881 aaaagcatac cgctcgaccc gaattatacg ttgaaagggg agttgctcgg cttttacgcg
     5941 cagctcgaag gtttaagtcg taataccagc cagcccaatg aaacggcgtt ggttagtgga
     6001 caattgacct ggaatgcgcc gtggggaagc gtatttggca gtggtggtta tttgcgccat
     6061 gcaatgaatg gtgccgtggt ggataccgac attggctatc ccttttcatt aagtcttgat
     6121 cgtaaccgtg aaggaatgca gtcctggcaa ttgggcgtca actatcgttt aacgccgcaa
     6181 tttacactga catttgcaca gattgtgact cgcggctatg aatccagtaa acgagatgtg
     6241 cggattgaag gcgcgggtat cttaggtggt atgaactatc gggtcagcga agggccgtta
     6301 caagggatga atttctttct tgctgccgat aaagggcggg aaaagcgcga tggcagtacg
     6361 ctgggcgatc gcctgaatta ctgggatgtg aaaatgagta ttcagtatga ctttatgctg
     6421 aagtcagaaa taacgccgga gagaaaaacc tccggcgttt cagattgttg acaaagtgcg
     6481 cgttttttat gccggatgcg gcgtaaacgc cttatccagc ctacaaaaac tcataaattc
     6541 aaagtgttgc aggaaaaggt aggcctgata agcgtagcgc atcaggcaat ctctggtttg
     6601 ttttcagatg aaaacgccga agtgaaaatt ctccggcgtt ttggccgtga attactgctg
     6661 cggaattgcc ggtacagccg gaacgttaag agctggcatc gcaaacatac caacgaaatc
     6721 ttccagtggc attttctgcc cgtttaacgt tatctgaccg ttagtatatt gcaggctggt
     6781 ggtgatggta ttgtcctgca aggtggtcag acggaacatc tgccccattg ctgatgcacc
     6841 ttcaacttgc tgtttcgcca gttttttcgc ttgatcttcc tgataacctt ccagcttcgc
     6901 tacctgagtc ataaactcag ttgccatatc caccggaatg gtcagtttcg catccagaga
     6961 tttaaccgaa cgatctactt cctgcgccag cgtttgcggc gcttctttag tcgttgccgg
     7021 atctttcagg aacagcgaca gattcagcgc actttcaccc tgactgtttt tccagcttag
     7081 cggcgcgata gtaatcaccg gatcgccttt caacatcagc ggcagggcgc taaagaaggc
     7141 ttccgtcact ttctcctgat aaagttcggg attgttggca atttctggct gtgccagcag
     7201 cgcctgagtt tgcgcgttat attgctggct aaactgatgc caggcttcac catcaatctg
     7261 gccgactttt aaagtcagct tgccgctgcc cagatcctga ttctgtacct tcaggctgtt
     7321 tagcgagtaa tccagttggc tattgatcgt tttaccgtca ttgaccagat ccgatttacc
     7381 gctgatctcc atgccttcca gcagtgccag ttctttgcct tccactgaaa tggtcatttt
     7441 ttccagtgac agtttttggt ttcctacgcg ctcaccaaaa cttgccagcg tgctggaacc
     7501 gtcggttttc agattattaa aggtcaactg cactttctgg ttgtattcgt taactgcgtc
     7561 tatccgacca ctttgcgcct ccccggaaag ggagatggct ttgccgtctc tgtcagcatt
     7621 taactggaac tcgccgccgc taaaggcgac tttttcatcc ttttgctcgt aattcagtgg
     7681 cttgagcgaa atatcggaac tggaatcacc gctgtaacca atgcgcgagt taatctcaaa
     7741 aggcgtttca ccttttgcca tatcaaacag tggtttgctt acttcgttat taaccagcgt
     7801 ggtttgaatt gatgccatcg acgggatcag gttcagtttt ttaagctggg caagcgggaa
     7861 gggaccatga tcaaccgatt cgttgaagat gacgctctga ccgcttttaa tccacggatt
     7921 ttctttcccg gcaatgggtt tcaccaacag ttgcaactgg ctgctgaata cgccgcgatg
     7981 atagttttga taactcactt ccaggttgga ttcaggagct gtcagtttga gttgcgcgtt
     8041 cgcctgcgcg accatgtctt cgagatgggt ttcaatcttc ttgcctgtat accatgcgcc
     8101 gcctgtccag actacgccta gcgcaacaat gacgcctacc gctaccagcg atttattcat
     8161 aatgattatc cataaaatga aatcaggcgg actggccgcc tgaaggtgtt ataagccttt
     8221 aataagctta gcaagagatg ttaatttttt cagtaagctc ttacagcttg ttgtaaacac
     8281 gcgctaaacg gccgtggcct ttgacagtca ccggtgattc gttggcggca ataaacgctg
     8341 attcacccgg tttaagctgt aactgctgag aacctttcca caacgttgca tcgccttcga
     8401 cgcagaacaa aatggcggca ctctgctggc taatggtggt ttctttatca ctaaggtcat
     8461 gcagcgagaa ggcaaaatca tccactggaa tcgggaagtc cagttctgca ccttgtttca
     8521 ccggctgggt caacaactgg ttagccggtt tggcttcgaa tttcacattg gcaaccagtt
     8581 ccggaatatc aatgtactta ggcgtcagac ccgcacgcag cacgttatcg gagttcgcca
     8641 tcacttccag cgccacgcct tgcaggtaag cgtgcggtgt ttcagcgaac aggaacatcg
     8701 cttcgccagg attcaatttc accacattca gtaacagcgg ggagaacagg ccactgtctt
     8761 ccgggtaaaa ttcagaaatt aaacgaatcg tttgccacgg ttcaccctgc tgactgtcga
     8821 gcgccgattt taaaatcgcc agcgcgcggg atttttcttc accctgcata ttcaacaggc
     8881 tggcgaacag ttcgcttaaa cgttcggcat taggctgttg taaaaagtga gcaattgccg
     8941 gatgtgcacc tgcgactggc tggagtaggg agacaatctc ggaaaactca cgaaacgcgt
     9001 tcatcgcaag gaaaggcgtc agcgcaaaaa ccagctccgg cttgtggtta ggatctttat
     9061 agttacgctc ggcggcatcc atcgggatac ctgcggcatt ttctttggca aaaccgattt
     9121 cagaattgcg tttgtttgga tgaacctgaa tggagagcgg ctgtgctgcg cataatactt
     9181 tgaacaggaa aggcagttcg ccaaagcgtt tggcaacggc ctctccgagc agagtcgatt
     9241 tatcactctc aatcacatca cgcagtgaaa cgatatctcc ggcggcattc tgcactcgtg
     9301 aactgctttt cggatgtgcg cccatccaca gctcggccat cggctggctg gacggatttt
     9361 ccataccata aagttcagtc aacgccgttt tgctgcccca ggcatagttt tgcactgagt
     9421 taatgagttt ttgcatgatc aatccctgtt ttaatgtgga aattaatccc actattaaag
     9481 caagaatcct acgggaagta acctggagcc gcaaaaagtc gtactagtct cagtttttgt
     9541 taaaaaagtg tgtaggatat tgttactcgc ttttaacagg gcaacggaac acccgcccag
     9601 agcataacca aaccaggcag taagtgagag aacaatgtca aacaaaccct ttcattatca
     9661 ggctcctttt ccactcaaaa aagatgatac tgagtattac ctgctaacca gcgaacacgt
     9721 tagcgtatct gaatttgaag ggcaggagat tttgaaagtc gcacccgaag cgttaactct
     9781 gttggcgcgt caggcgtttc atgatgcgtc atttatgctg cgtccggctc accaacaaca
     9841 ggtggccgac attctgcgtg acccggaagc cagcgaaaat gataaatatg tggcgctgca
     9901 attcctgcgc aactccgaca tcgcggcgaa aggtgttctg ccaacctgtc aggataccgg
     9961 cactgcgatt attgttggta aaaaagggca gcgtgtatgg accggtggct gtgatgaagc
    10021 ggcgctggcg cgcggtgtct ataacactta tatcgaagat aatttgcgct actcgcaaaa
    10081 cgcgccgctg gatatgtata aagaggtgaa taccggcacc aatctgccag cgcagatcga
    10141 tctttatgcc gttgatggcg acgagtacaa attcctctgt atcgccaaag gtggtggttc
    10201 ggcaaacaag acgtatctct atcaggaaac caaagcgtta ctaacgccgg ggaaactgaa
    10261 aaattacctg gttgagaaga tgcgcacgct gggtacggcg gcctgtcctc cgtatcatat
    10321 tgcgttcgta attggtggaa cttctgcaga aactaacctt aaaacagtga aactggcttc
    10381 cgctaaatac tatgacgaac tgccaacgga agggaatgag cacggtcagg cattccgcga
    10441 tgtggaactg gaaaaagaat tgctgatcga agcgcaaaat ctcggtctgg gtgcgcagtt
    10501 tggtggtaaa tacttcgctc acgacatacg cgtgattcgc ctgccacgtc acggcgcatc
    10561 ctgtccggtt ggtatgggtg tttcctgctc tgctgaccgt aatatcaaag cgaagatcaa
    10621 ccgtcagggg atctggatcg aaaaactgga acataatcca ggtaaatata tccctgaaga
    10681 gctgcgtaaa gcgggagaag gcgaagcggt gcgcgttgac cttaaccgtc cgatgaaaga
    10741 gatcctcgca cagttgtcgc agtatcccgt ttctacacgc ttatcgctta acggcacgat
    10801 tatcgtcggt cgtgatattg ctcacgccaa actgaaagag cggatggata acggtgaagg
    10861 gctgccgcag tacattaaag atcatccgat ttactacgcg ggtccggcca aaacgccgga
    10921 aggttatgcc tccggttctc ttggcccaac gaccgccgga cggatggatt cttatgtcga
    10981 tcaactgcaa gcgcagggcg gaagtatgat catgctggcg aaaggcaacc gcagccagca
    11041 ggtgacggat gcctgtaaaa aacacggcgg cttctacctt ggcagtatcg gtggtccggc
    11101 cgctgtattg gcgcagggaa gtattaagag cctggaatgt gttgaatatc cggaactggg
    11161 aatggaagcc atctggaaaa ttgaagtgga agatttcccg gcgtttatcc ttgtggatga
    11221 taaaggaaat gacttcttcc agcagataca actcacacag tgcactcgct gtgtgaaata
    11281 aacagagccg ccctt
//
LOCUS       DAZPDU010000140        11294 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZPDU010000140 DAZPDU010000000
VERSION     DAZPDU010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11294
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.142"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            79..213
                     /locus_tag="ACHURS_003308"
     CDS             79..213
                     /locus_tag="ACHURS_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFR1238961.1"
                     /translation="MIYYLGYDRFLLIFFTDYSHFMDRITLPSTDTARGKGKWKIITA
                     "
     gene            192..296
                     /gene="speFL"
                     /locus_tag="ACHURS_003309"
     CDS             192..296
                     /gene="speFL"
                     /locus_tag="ACHURS_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFR1238962.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            607..2805
                     /gene="speF"
                     /locus_tag="ACHURS_003310"
     CDS             607..2805
                     /gene="speF"
                     /locus_tag="ACHURS_003310"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFR1238963.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVNDVE
                     RGKLDEIDATGYGIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGETLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKAHFPHV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2802..4121
                     /gene="potE"
                     /locus_tag="ACHURS_003311"
     CDS             2802..4121
                     /gene="potE"
                     /locus_tag="ACHURS_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFR1238964.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4177..5817)
                     /gene="pgm"
                     /locus_tag="ACHURS_003312"
     CDS             complement(4177..5817)
                     /gene="pgm"
                     /locus_tag="ACHURS_003312"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFR1238965.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQQFVEGLADI
                     VDMAAIQKTGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAVLSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5843..6388)
                     /gene="seqA"
                     /locus_tag="ACHURS_003313"
     CDS             complement(5843..6388)
                     /gene="seqA"
                     /locus_tag="ACHURS_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFR1238966.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6573..7337
                     /gene="ybfF"
                     /locus_tag="ACHURS_003314"
     CDS             6573..7337
                     /gene="ybfF"
                     /locus_tag="ACHURS_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR1238967.1"
                     /translation="MKLNIRAQTAQNQHNNSPIALVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     RDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMHQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7477..7770
                     /gene="ybfE"
                     /locus_tag="ACHURS_003315"
     CDS             7477..7770
                     /gene="ybfE"
                     /locus_tag="ACHURS_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFR1238968.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7910..8440
                     /gene="fldA"
                     /locus_tag="ACHURS_003316"
     CDS             7910..8440
                     /gene="fldA"
                     /locus_tag="ACHURS_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFR1238969.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8650..8736
                     /gene="uof"
                     /locus_tag="ACHURS_003317"
     CDS             8650..8736
                     /gene="uof"
                     /locus_tag="ACHURS_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFR1238970.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8729..9175
                     /gene="fur"
                     /locus_tag="ACHURS_003318"
     CDS             8729..9175
                     /gene="fur"
                     /locus_tag="ACHURS_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFR1238971.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9260..9586)
                     /gene="chiQ"
                     /locus_tag="ACHURS_003319"
     CDS             complement(9260..9586)
                     /gene="chiQ"
                     /locus_tag="ACHURS_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFR1238972.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQHFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9636..11042)
                     /gene="chiP"
                     /locus_tag="ACHURS_003320"
                     /pseudo
     CDS             complement(9636..11042)
                     /gene="chiP"
                     /locus_tag="ACHURS_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
BASE COUNT         2764 a         2779 c         2808 g         2943 t
ORIGIN      
        1 tcttaacgca aatataaaac acgaaattac aacatttaag taacaaaaca gcatctaata
       61 tggcgatatt cataattaat gatttattat ctggggtatg atcgtttttt gttgatcttc
      121 ttcacagatt atagccattt catggataga ataactctac cttcaactga cacagcaaga
      181 ggtaaaggta aatggaaaat aataaccgct taatgcctca tataaggcgg acaacccata
      241 tcatgaagtt tgcccatcgt aatagttttg actttcattt ctttaatgcc cgctagtctt
      301 ctgactaaag ggcaccccaa cgtacaggtc tccctgactt taagcattac aggttaatac
      361 ctgtattcct cggtgctcat atactgctaa cccttttaaa ctctaaataa ttcgagtcgc
      421 agcacttgca acttgaggta tgacgagtat agccagttac cgggctggtc tgggttattg
      481 catctgcaaa aagcaaacta ctgatttatt tatcagcggt ggagctttgc tttttttccg
      541 gcgtgatcga tttctccttt gagaaattga ggacctgcta ttacctgaaa taaagagatg
      601 aataaaatgt cagaattaaa aattgccgtt agccgttctt gcccggattg tttttccact
      661 catcgtgcat gcgtgaatat tgacgaaagt aattatattg acgttgccgc cattatttta
      721 tcagtcaatg atgttgaacg tggaaaactc gatgaaatag acgctactgg ctatggcatt
      781 cctgttttta ttgcaacgga aaatgaagaa cgtgtaccag cagaatatct gccacgtatt
      841 tctggtgtct tcgagcattg cgaatcacgg aaagagtttt atggacgtca gttagaaacc
      901 gctgctagcc attatgaaac gcaactgcgc ccgcccttct tccgcgcact ggtcgattat
      961 gtcaatcagg gcaatagcgc ttttgactgc ccgggtcatc agggaggtga gtttttccgt
     1021 cgtcatccgg cagggaatca gttcgtggag tacttcggag aaacgctgtt ccgttccgac
     1081 ttatgcaacg cagacgtagc aatgggcgat ctgctgattc acgaaggcgc gccatgcatt
     1141 gcacagcaac atgcggcaaa agtgtttaat gccgataaaa catacttcgt tctaaatggc
     1201 acttcatctt ctaacaaagt ggttttaaac gccctgctaa caccgggtga tctggtgctg
     1261 tttgatcgca ataaccacaa atctaaccac cacggagcgt tgctacaggc tggtgcaaca
     1321 ccggtttatc tggaaacggc acgtaacccg tatggcttta tcggtggcat tgatgcgcac
     1381 tgttttgaag aaagttacct gcgtgagctg atcgcggaag tcgcaccgca gcgggcaaaa
     1441 gaggctcgtc ctttccgcct cgctgtgatt cagttaggca cctacgacgg tacgatttat
     1501 aacgcccgcc aggtggtgga taaaattggt catctgtgtg actacattct gtttgactcc
     1561 gcatgggttg gctatgagca atttatcccg atgatggctg actgttcgcc gctgttgctg
     1621 gatcttaatg agaacgatcc gggtattctg gttacgcaat ccgtacataa acagcaggct
     1681 ggtttttctc aaacttcaca aatccataaa aaagacagcc acatcaaagg gcaacagcgt
     1741 tatgtaccgc acaaacgcat gaacaacgcc tttatgatgc acgcctccac cagcccgttc
     1801 tatccgctgt ttgccgcact ggatatcaac gccaaaatgc atgaaggtgt cagcggtcgt
     1861 aatatgtgga tggattgtgt ggtaaatggc attaatgccc gcaaactgat cctcgataac
     1921 tgtcagcata ttcgtccgtt cgtacctgaa ctggtggatg gtaaaccctg gcagtcgtat
     1981 gaaacagcgc aaattgcggt tgatctgcgc ttcttccagt ttgtaccagg ggaacactgg
     2041 cattcttttg aaggctatgc agagaatcaa tactttgtcg atccttgcaa actgttgctg
     2101 acaaccccag gtattgatgc acgtaacggc gaatatgaag cgttcggtgt acccgcgacg
     2161 attcttgcta acttcctgcg cgaaaatggc gtagtgccgg aaaaatgcga tcttaactcc
     2221 atcctcttcc tgctgactcc ggcagaagat atggccaaac ttcagcaact tgttgccctg
     2281 ctggtacgct tcgaaaaact gcttgagtcc gacgcgccat tagcagaagt gctgccttcc
     2341 atctacaaac agcatgaaga gcgctacgcc ggttataccc tgcgtcagtt gtgccaggaa
     2401 atgcatgatt tgtatgcccg acacaacgtg aaacaactgc aaaaagagat gttccgcaaa
     2461 gcgcacttcc cacacgtcag catgaatccg caagaagcca actacgccta tttacgcggt
     2521 gaagtggagc tggttcgtct gccggatgcc gaaggtcgta tcgctgccga aggggcgctt
     2581 ccttatcctc cgggtgtgct gtgtgttgtt ccgggtgaaa tctggggtgg tgctgttttg
     2641 cgttacttca gcgctctgga agaagggatc aacctgctgc caggttttgc accggagctg
     2701 cagggtgtct atatcgaaga acatgatggt cgtaagcaag tttggtgcta tgtcatcaag
     2761 cctcgtgatg cgcaaagcac cctgttgaaa ggggaaaaat tatgagtcag gctaaatcga
     2821 acaaaatggg cgtcgttcag ttaaccatac tgacgatggt caacatgatg ggctccggta
     2881 tcatcatgct gccgacaaag cttgccgaag ttgggacaat ttcaattatc tcctggctgg
     2941 tgacagccgt aggctcaatg gcactggcat gggcattcgc aaaatgcggt atgttcagcc
     3001 gtaaatcagg tggtatgggc ggttatgccg agtatgcttt cggtaaatcc ggtaacttta
     3061 tggcgaacta tacctacggc gtctcactgc tgattgctaa cgtcgcgatt gctatttcgg
     3121 cggttggtta cggcaccgaa ctgctcggcg cgagtttgtc gccagtgcag attggtcttg
     3181 cgaccatcgg ggtgctgtgg atttgtaccg tggctaactt tggtggtgcg cgcattaccg
     3241 ggcaaatcag tagcattacc gtgtgggggg tcattattcc ggtggtaggc ctgtgcatta
     3301 tcggctggtt ctggtttagc ccgacgcttt acgttgattc ctggaatccg catcatgcac
     3361 cgttcttcag tgcagtaggt tcttccatcg ctatgacgct gtgggctttt ctcggtctgg
     3421 agtctgcgtg tgcgaatact gatgtagtgg aaaacccgga acgtaatgtg ccaatcgcgg
     3481 tactcggcgg tacgttaggt gcggcggtga tttatatcgt ctccaccaac gtgattgcag
     3541 ggattgtgcc taatatggag ctggcaaatt caacggcacc atttggcctg gccttcgcac
     3601 agatgttcac accagaagtg ggtaaagtca ttatggcgct gatggtgatg tcctgctgcg
     3661 gttcgctgct tggctggcag ttcaccattg cccaggtgtt taagtcttca tctgatgaag
     3721 gctacttccc taaaattttc tcccgtgtaa ccaaagtgga tgcaccggtg cagggaatgt
     3781 tgaccattgt gattatccag agcgggttgg cgctgatgac gattagcccg tcgctaaaca
     3841 gtcagttcaa cgtgctggtt aacctggccg tggtgaccaa tatcattccg tatattctgt
     3901 caatggcggc attagtcatt attcagaagg tcgctaatgt gcccccctca aaagcgaaag
     3961 ttgcaaactt tgttgctttt gttggcgcga tgtatagctt ttatgcgctg tactcatccg
     4021 gggaagaagc catgctgtac ggttccatcg tgaccttcct tggttggaca ctgtatggtc
     4081 tggtatcacc acgctttgaa ctgaaaaata aacacggtta aaaaagcaaa gtaaaaaagg
     4141 gcgatcttgc gaccgccctt tttttattaa atgtgtttac gcgtttttca gaacttcgct
     4201 aacaatctca accgcttctt tctcaatctg cttgcgatgt tcttcaccga ggaagctttc
     4261 gcagtagatc ttatatgcgt cttccgtgcc tgacggacgc gcggcgaacc agccgttgtc
     4321 agtcatcact ttcagaccgc cgatagaagc accattgccc ggtgccgccg tcaggcgcgc
     4381 ggtgatcggg tcgcctgcca gggtgctggc gctcaccatt tccggagaca gcttagacag
     4441 caccgctttt tgtgcggaag tcgcagctgc ctgcaaacgg ttgtagctcg gcgcaccaaa
     4501 gcgttttgcc agttcgttgt agtgttcctg cgggttctta ccggtgacag cggtgatttc
     4561 cgccgccagc agacacatga tgatgccgtc tttgtcggtg gaccacggcg tgccgtcgaa
     4621 acgcaggaag gaagcccctg cgctctcttc gccgccaaag ccgaagctgc cgtcgaacag
     4681 accatcaaca aaccatttga aacctaccgg gacttctacc agcttgcggc ccaagtcatt
     4741 gaccacacgg tcgatcatcg cagaagaaac cagcgtttta ccgacagcaa catctttgcc
     4801 ccactgcgga cgatgctgga acaggtagtt gatcgccacc gccaggtagt ggttcggatt
     4861 catcaaacct gccggagtga cgataccgtg acggtcataa tccgggtcgt tagcaaacgc
     4921 cagatcgaac ttatcacgca atgccagcag gcccgccatc gcacactcgg aggagcagtc
     4981 catacggatc gcgccgtctt tatcgaggtg cataaagcgg aaggtttgat cgacctgatc
     5041 gttaacgatg gtcaggttga ggttgtaata ctcgccaatt cgcttccagt attcgatacc
     5101 ggaaccaccc agcggatcaa cgcccagcgt caggcccgtt ttctgaatcg ccgccatatc
     5161 aacgatatcg gccagacctt ccacaaactg ctgcaccaga tcctgctctt tcacatgacc
     5221 ggatgccatc gcttcgtcga gggagatacg cttcacgcct ttcaggccat cggccatcag
     5281 tgcgttggca cgatcttcca ccactttagt gacgttggta tcagccgggc caccatttgg
     5341 cggattgtac ttgataccac catcttccgg cgggttatgg gagggtgtaa tcacgatacc
     5401 gtctgccagc gggccacctt ttttattatg aaccaggatg gcattggaaa cggcaggcgt
     5461 cggagtgaag ccattgtttt cctgcacaat gacatcaacg ccgttcgctg ccagcacttc
     5521 cagtacggaa atgaacgcag gctcggacag ggcgtgagta tctttaccca catagcaagg
     5581 gccagtgatg ccgtttttcg cacgttcttc agcaattgcc tgagcgatcg ccagaatgtg
     5641 cggctcgtta aagctgtggc gcgctgcact gccacggtga ccggaagtac cgaatttcac
     5701 cgcgtgctcc gcattccctg cttctggttt cagtacataa tattgcgccg tcagttgggc
     5761 gacgttaatc aaatcactct gttgtgcagg ttggcctgca cgattgtgga ttgccattgc
     5821 tttgtccttt gtctgcaacg ttttagatag ttccgcaaac cttctcaatc aattccgccg
     5881 ggaattgcat cgactgcatg atgtgttcga tcatgctgca tttacggccg gtgttggtgt
     5941 tggtgatcac ccaatacggc gtgcctggca catgtttcgg cttggtctga ttaccatttt
     6001 tcagcagcgt ttgttcatct gccgcaaagt aaacgcgtgt acgaccgtgc aacgattccg
     6061 ttgcttcggc aaacgcctgg gcgtcaagag aatatagtgt agacaacagc agcataaagc
     6121 gattgaccgc tcgcttttgc tctgcgtatt cgtcagaaag cagaagttca cgcattgcgc
     6181 gaaccttgtc tttaatcgtt ttgaccggct tcgcttcgac gatagcaggt gacgcaacgc
     6241 gaacctcttt cgtcaccgga gcagcaggct gtgatgcggc ggaaaatttc aacatacgcc
     6301 gtaaaatgtc ggatgcgctc tcgccgatat gcttagtgtg gctggcaata tagctgtaga
     6361 gttcatcatc aacttcaatc gttttcatct taatccagta cagtgtctta gctgaataca
     6421 agtcgccaag agtatatgaa taaatcccgg cagcggatag cgtcaagcct ggcttgcaga
     6481 aaaactcgga ataaacctta tttgttgcag ccctgtggca caatggtcaa cataataccc
     6541 taacccgaca acgcgaaaaa aagaactttg ccatgaaatt gaatatccgc gcgcaaactg
     6601 cacaaaacca gcacaataat tctcccatcg ctcttgtcca tggtctgttt ggtagcctcg
     6661 acaaccttgg cgtactggct cgcgatctgg taaacgatca caatatcatc caggttgata
     6721 tgcgtaacca cggtctttca ccgagagatc cggtaatgaa ttacccggcg atggcgcagg
     6781 atcttgttga tactctggat gcacagcaga tcgacaaagc aacatttatc ggtcactcca
     6841 tgggcggtaa agcggtaatg gcacttacgg cactggcccg cgatcgcatc gataaactgg
     6901 tggcgatcga tatcgcaccg gtcgactatc acgtacgccg tcatgatgag attttcgcgg
     6961 ctatcaacgc ggtcagtgaa tcggacgcac aaactcgcca gcaagcagca gcaataatgc
     7021 accagcatct taatgaagaa ggggtgattc agtttctgtt gaaatctttt gttgacgggg
     7081 agtggcgctt taacgtgccg gtattgtggg atcagtatcc gcatattgtg ggttgggaga
     7141 aaatcccggc atgggatcac cctgccctgt ttatccctgg cggcaattct ccgtatgtta
     7201 gcgagcagta ccgtgatgat ttactggcac aatttccaca ggcacgggcg catgtgattg
     7261 caggcgcggg tcactgggtc catgctgaaa aaccggatgc ggtattacgc gctatccgtc
     7321 gctatctcaa tgattaacgc aactgattaa aaacccagcg tccgcgtcgc tttccgcgcg
     7381 tgggcgttgg cgtgactcat cgcgctgatg tattatggcg cgctatccat ccgggcagag
     7441 gcctggctga ttgtttcccc cgaagtcacc aagatcatgg ccaaagaaca aacggaccgt
     7501 acgacattag atctgttcgc gcacgagcgt cgaccgggac gaccgaaaac taatccgctt
     7561 tcgcgcgatg aacagctgcg tattaataaa cgcaaccagc taaaacgcga caaagtacgt
     7621 ggccttaagc gtgtcgaact gaagctgaac gcggaagctg tcgaggcgct gaacgagctg
     7681 gcggagtcgc gcaatatgag ccgtagcgaa ctgatcgaag agatgttaat gcagcaactg
     7741 gcggcgctgc gtagtcaggg catcgtttaa atttccactt tcatgtagca cagtgtgcag
     7801 tcctgctcgt ttgctgattc cgcaagactg cctgttctgc tatgattgcc tttatccgtg
     7861 ggcaattttc cacccccatt tcaataagtt tcaagaggtt atttcactca tggctatcac
     7921 tggcatcttt ttcggcagcg acaccggtaa taccgaaaat atcgcaaaaa tgattcaaaa
     7981 acagcttggt aaagacgttg ccgatgtcca tgacattgca aaaagcagca aagaagatct
     8041 ggaagcttat gacattctgc tgctgggcat cccaacctgg tattatggcg aagcgcagtg
     8101 tgactgggat gacttcttcc cgactctcga agagattgat ttcaacggca aactggttgc
     8161 gctgtttggt tgtggtgacc aggaagatta cgccgaatat ttctgcgacg cattgggcac
     8221 catccgcgac atcattgaac cgcgcggtgc aacgatcgtt ggtcactggc caactgcggg
     8281 ctatcatttc gaagcatcaa aaggtctggc tgatgacgac cactttgtcg gtctggctat
     8341 cgacgaagac cgtcagccgg aactgaccgc tgaacgtgta gaaaaatggg ttaaacagat
     8401 ttctgaagag ttgcatctcg acgaaattct caatgcctga tgtgatgcgg cgtagactca
     8461 tgtctacgcc gtattaatag ataatgccaa tcaaaataat tgctacaaat ttgtaacttt
     8521 tgctgttgta cctgtacaat gtcccggtgt tcaagtggcc ttgccgttgt aaatgtaagc
     8581 tgtgccacgt ttttattaac aatatttgcc agggacttgt ggttttcatt taggcgtggc
     8641 aattctataa tgatacgcat tatctcaaga gcaaattctg tcacttcttc taatgaagtg
     8701 aaccgcttag taacaggaca gattccgcat gactgataac aataccgccc taaagaaagc
     8761 tggcctgaaa gtaacgcttc ctcgtttaaa aatcctagaa gttcttcagg agccggacaa
     8821 ccatcacgtc agtgcggaag atttatacaa acgtctgatc gatatgggtg aagaaattgg
     8881 tctggctacg gtatatcgcg tactgaacca gtttgacgac gctggtatcg tcacccgcca
     8941 caattttgaa ggcggtaaat ccgtatttga actgacacag caacatcacc acgatcacct
     9001 gatctgcctc gactgcggca aggttatcga atttagtgat gattccatcg aagcgcgtca
     9061 gcgtgaaatt gccgcaaaac atggcattcg cctgactaac cacagtctct atctttacgg
     9121 tcactgtgcc gaaggcgatt gccgcgaaga tgaacatgcg cacgaaggca aataagccag
     9181 cctgaatgag aaaaagccaa cctgcgggtt ggctttttta tgcaagggaa ataagaaaag
     9241 cgagagttac agctctcact tatttgttat tactgcgaat ttcctgccaa actttatcac
     9301 aatcggcttt caccgcctga tcatttccgg tttgcgttgc acgcagacac tgctgataat
     9361 ccagtacgcg gacactttcc tggtcggcaa aatgctgatg ttgtttctct ttcttcagca
     9421 cgtttaacac gctctggcag gcttcaattt tttctggcga accttgtgcg gtgttgatac
     9481 aggcgctgta tgcctctttc agacgactgt cttcctgtgg cgcggttgat tgcgcacaag
     9541 ctaccagccc cgatgccatt atggcgatga gaatcagttt tttcatagca aattcctcaa
     9601 acgtgcggca ggcacaaaac ctgccgcgtc gggcatcaga agatggtgaa tggtgcgatt
     9661 accataaatt tcacgtcacg ctcatcctgg aagatgttgc cgtaaccacc gccccagctt
     9721 gggatgtcgg agtggttgtc gtattcggtg aagtgcagtt tgaacatcgt gcctttggcg
     9781 cgaccgtcct gaatggtgta gaccgcatcc aggctgtatg cagactcttc aatagtccgg
     9841 tttttgtcgt agtacgcatc cggattgctc tgctaggtcg caggtttagc atcccatgcg
     9901 taaacgtagg aagcgccgat ggcgaagcct ggaagattcc agtttttcag gtcatacatc
     9961 gcaccgaaga agaccgcttt ttcgccgtta gcgttgaagt cggaacggtt atcccaccag
    10021 atatccaggc ggccgttgga ggaagcgtag gttggagtca tacgttgcag gaagtatccc
    10081 tgctgaccgt cagccttaac ccaggtgcct tcgaggcgca aatccactac gtcagccgcc
    10141 cggtaaccaa aggtcaacgc ttgcagccag gcggtgccgt cataaaggtc gttgacgctg
    10201 cgatcgtcaa ctttatcgcg cgtaccatag aactggtagc tggtggttaa cgggctaccg
    10261 gcgatatcaa atttgtagct ggctttggca aaatattgat cgatataccc ttccgcctga
    10321 ccaaatgccg cttccagtac gaagttattt ttgaagtcgt atttcgcccc aagggagtgc
    10381 agataatcaa ctttggtggt tttatcgttc tgataaaact catccatttc cagatgccac
    10441 ggcgctttgt attcgttggt ccacatgtag gagaaactca acgcaccagc atcgccgtaa
    10501 tcaaaattcg ccccggcttc cgccccctga taagtacctg gcataaagct ccagtgcggc
    10561 gctaacagcg tttgaccagt tggctgaatg taacctgccc tcgcccaaac cggaccgtat
    10621 ttaaatttgg ccgcagcttt atacaggctt ataccgcttt tgtcgccgga ccagtcttcg
    10681 tcataggctt tattactttt tgaaaacgcg atttcgttcg ggtgggagct gtcgccgttt
    10741 tccgccattt caatcgccgt aaacgcagca atatcaaggc cgaacatatc agcagcataa
    10801 ccggactgaa aatcgaggtt ggcattccag gtggagtgag aaaggttggt tttgtatttg
    10861 tcgccgtcgg taacatcttt acggtcgcgt tcacgctgcc agtaatatat accgccggtt
    10921 aaggttgaat cgtcgatgaa tccttcggcg cgagcctccg gcattgccat aaagcccgac
    10981 attgctgtaa caccggcgat agccagcgcc agcgtactat gtttgccact aaacgtacgc
    11041 atgggttaat cctctttgac gtataaattg ctgcaccaaa ggtgaacagc gaaaaaataa
    11101 aaattaaaaa agtagcgttg agaaaagtct cgctgactac aggaacagac tatttttcgc
    11161 gacgcgaatt atcaatctct ttctgtaacc agaatgtaag aacatgagca accgcacata
    11221 ttttgatgat ttttgttaca aggcttaagt ttgcggttga aaggcttgca gaagaataaa
    11281 agcgtgaaat tcca
//
LOCUS       DAZPDU010000141        11246 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZPDU010000141 DAZPDU010000000
VERSION     DAZPDU010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11246
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.143"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..1760
                     /locus_tag="ACHURS_003321"
     CDS             <1..1760
                     /locus_tag="ACHURS_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFR1238973.1"
                     /translation="VYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEI
                     ITPKKPGEAILHAFNATYQQIRENMSEFARCHYGYIQIPPVTTFRADGPETPEEEKGY
                     WFHAYQPEDLCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDY
                     PSADIRAKISKDIEGGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKD
                     NISKDEWENLHALKEKRLNGIPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHS
                     KHEQIERSITPPLKNYNERLHNTNLFNPKYHKKAVMNYMEYLVTCQLDEQQMSSLLYE
                     RDDLSAIDFSLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGN
                     LFNDFLFNHQQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWL
                     NKGCSTYQQLYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVI
                     INKGNEEVEGTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPGKVFTLRGGTLNINIP
                     GRSALLLGKTGEPPNYLYL"
     gene            2096..3073
                     /gene="glaH"
                     /locus_tag="ACHURS_003322"
     CDS             2096..3073
                     /gene="glaH"
                     /locus_tag="ACHURS_003322"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFR1238974.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            3093..4361
                     /gene="lhgO"
                     /locus_tag="ACHURS_003323"
     CDS             3093..4361
                     /gene="lhgO"
                     /locus_tag="ACHURS_003323"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFR1238975.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            4384..5832
                     /gene="gabD"
                     /locus_tag="ACHURS_003324"
     CDS             4384..5832
                     /gene="gabD"
                     /locus_tag="ACHURS_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFR1238976.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGGIIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            5846..7126
                     /gene="gabT"
                     /locus_tag="ACHURS_003325"
     CDS             5846..7126
                     /gene="gabT"
                     /locus_tag="ACHURS_003325"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFR1238977.1"
                     /translation="MNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEHENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            7437..8837
                     /gene="gabP"
                     /locus_tag="ACHURS_003326"
     CDS             7437..8837
                     /gene="gabP"
                     /locus_tag="ACHURS_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFR1238978.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            8858..9520
                     /gene="csiR"
                     /locus_tag="ACHURS_003327"
     CDS             8858..9520
                     /gene="csiR"
                     /locus_tag="ACHURS_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFR1238979.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(9521..9970)
                     /gene="kbp"
                     /locus_tag="ACHURS_003328"
     CDS             complement(9521..9970)
                     /gene="kbp"
                     /locus_tag="ACHURS_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFR1238980.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(10054..10212)
                     /locus_tag="ACHURS_003329"
     CDS             complement(10054..10212)
                     /locus_tag="ACHURS_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFR1238981.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            10395..10694
                     /locus_tag="ACHURS_003330"
     CDS             10395..10694
                     /locus_tag="ACHURS_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFR1238982.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            10704..11228
                     /gene="ygaP"
                     /locus_tag="ACHURS_003331"
     CDS             10704..11228
                     /gene="ygaP"
                     /locus_tag="ACHURS_003331"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFR1238983.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
BASE COUNT         2786 a         2785 c         2939 g         2736 t
ORIGIN      
        1 atgtatataa tattaacgtt acgtcacctt ccggacagca agaaacattt aaaatactaa
       61 tcaatccaga acatctacgg cagacacttg agcgtaaatc tcttactgct gttcagaaat
      121 cacaatgtga aatcatcacc cctaaaaaac ccggcgaagc gattcttcat gcttttaatg
      181 ccacctacca gcaaatcagg gaaaatatgt ctgagtttgc acgttgccat tatgggtata
      241 tacaaatccc tccagtgaca actttccgcg ccgacggacc agaaactccc gaagaagaaa
      301 agggttactg gtttcatgct tatcaacccg aagatctttg taccatccat aatccaatgg
      361 gagatttgca ggattttatt gcattggtta aagatgctaa aaaatttggt atcgatatca
      421 ttcctgatta tacctttaac tttatgggaa ttgggggtag tggtaaaaat gacctggatt
      481 atccctctgc tgatatacga gcgaagatca gtaaagatat agaaggtggt atccctggct
      541 attggcaagg tcaggttttg attccattca ttaaagatcc agtaacaaaa gaacgtaaac
      601 aaatccatcc agaagatata catctcactg caaaagactt cgaagcaagt aaagataaca
      661 tctctaagga tgaatgggaa aacctccatg cattaaaaga aaagcgttta aatggaattc
      721 ctaaaacaac acccaaaagt gaccaggtta ttatgttgca aaatcaatac gttcgtgaaa
      781 tgcgaaaata tggcgtacga ggtttacgtt atgatgcggc aaaacactca aaacatgaac
      841 aaatagaaag atcaataacc ccaccgctta aaaattataa tgagcggtta cacaatacta
      901 acttatttaa cccaaaatat cataaaaaag ccgttatgaa ttacatggaa tatctggtaa
      961 cttgtcagtt ggatgaacaa caaatgtcat cactgcttta tgaaagagat gatttaagcg
     1021 ccattgattt ttcattgctc atgaagacga taaaagcctt ttcattcggg ggagatctcc
     1081 taacccttgc atcaaaaccg ggttccacaa tctcaagcat cccatcagaa agacggatat
     1141 tgattaacat taaccacgat tttcctaaca atggcaatct tttcaatgac tttctattta
     1201 accatcaaca agatgaacaa ttagcaatgg catatatggc cgctctcccg ttcagcagac
     1261 ctttagttta ctgggatggc caggtattaa aatcaacgac tgaaattaaa aattatgatg
     1321 ggtccacgcg tgtcggtggt gaggcgtggc ttaataaagg ttgctctacc tatcagcagc
     1381 tctacaatga attccacgca ttatatatag ataaagcagg aatatggagc gcatttgagg
     1441 gtgtatttgc aactaaaaac gttctggcct ttagtcgtgg ggattctgtg aacattaatc
     1501 actctcctca tgatggacta gttataataa ataaaggaaa cgaagaagtt gaaggtacct
     1561 ggcctaacaa attgcaacct ggaatatata aaaacatggg gagtaatagc gttaacatta
     1621 ttattaataa tacccgaaaa attatccccc ctggtaaagt atttacgctt agaggcggaa
     1681 ctctaaatat caatattcct gggcgtagcg ctcttctttt agggaaaaca ggagaaccgc
     1741 cgaactatct ctatttataa tttaatgtta tatctgcccc gataaaacgg ggcagataat
     1801 atgtttagtt tactaacggt cattttgcag tgaagccatt tactgttttt tatcgaccag
     1861 ataatctgtt ctctaatgtt aactccccct aacctgttgc tttagttatt catttcctgt
     1921 ctcactttgc cttaatactc tacgttaaat gttactaatt tgttgctttt gatcacaata
     1981 agaaaacaat atgtcgcttt tgtgcgcatt tttcagaaat gtagatattt ttagattatg
     2041 gctacgaaat gagcatcgcc atgtcaccct acatctcata agaggatcgc ttctgatgaa
     2101 tgcactgacc gccgtacaaa ataacgctgt cgattcaggc caggactata gcggattcac
     2161 tctcatcccg tcggcgcaat ccccgcgtct gctggaactc accttcaccg aacagacgac
     2221 caaacagttt ctcgagcagg ttgccgaatg gcccgtacag gcgctggagt acaaatcgtt
     2281 tctgcgtttt cgggtaggca aaattcttga cgatctgtgt gcgaatcagc tgcaaccatt
     2341 gctgttgaag accctgctaa accgtgctga aggtgcgctg ttgatcaatg cggtgggtat
     2401 cgatgatgtg gcgcaggcag atgagatggt gaagctggca acggcggtag cgcatctgat
     2461 tggtcgctca aatttcgatg cgatgagcgg tcagtattac gcgcgtttcg tggtgaaaaa
     2521 tgtcgataac tcagacagct atctgcgtca gccgcaccgc gtgatggaac tgcacaacga
     2581 cggcacgtat gtcgaagaga tcaccgatta cgtgctgatg atgaaaatcg atgagcaaaa
     2641 catgcagggt ggaaattcgt tgctgctgca tctcgatgac tgggaacatc tggaccacta
     2701 tttccgccat ccactggcgc gtcgcccgat gcgctttgcc gcgccgccga gcaaaaacgt
     2761 cagcaaagat gttttccatc cagtgttcga cgttgatcaa cagggtcgcc cggtgatgcg
     2821 ctatatcgac cagttcgtcc agccaaaaga ttttgaagaa ggcgtgtggt tgagcgagct
     2881 ttccgatgcc attgaaacca gcaaaggcat tctttctgta cccgttcccg ttggcaaatt
     2941 cctgttgata aacaacctgt tctggctgca cggtcgcgac cgctttactc cgcacccgga
     3001 tctgcgccgt gaactgatgc gtcagcgtgg ctatttcgct tacgccactc accactacca
     3061 gacgcatcag taagcgcaaa ggaattgagc ggatgtatga ttttgtgatt attggcggcg
     3121 gcatcatcgg catgtcgacc gccatgcaac tgattgatgt ttatccggat gcccgcattg
     3181 cgttgctgga aaaagagtcc ggcccggcct gtcaccagac gggccacaac agcggcgtga
     3241 tccatgccgg ggtctattac acgcccggca gcctgaaggc gcagttttgc ctggcgggga
     3301 accgcgccac caaagccttt tgcgatcaaa acggcattcg ctatgacaac tgcggcaaga
     3361 tgctggtcgc cacgtctcag ctcgaaatgg aacggatgcg cgccttatgg gaacgcacag
     3421 cggcgaacgg tatcgagcgc gagtggataa acgccgtgga actgcgcgag cgcgaaccga
     3481 atatcaccgg gcttggtggc atttttgtgc cgtccagcgg cattgtcagc taccgcgaag
     3541 tcacagcggc aatggcaaaa atcttccagg ccagaggcgg cgagattatt tataacgccg
     3601 aagtcagcgc cctcagtgag cataaaaacg gcgtggtgat acgcacccgt cagggcggtg
     3661 aatatgaagc atcgacgctg attagctgtt ccgggctgat ggctgaccgg ctggtgaaaa
     3721 tgctcggcct cgaaccgggc tttatcatct gcccgttccg tggtgagtat ttccgccttg
     3781 cgccggagca taaccagatt gttaaccacc tgatttaccc cattcccgac cccgcgatgc
     3841 catttttggg cgtgcatctc acccgcatga tcgacggcag cgtgaccgtc gggccaaacg
     3901 cggtgctggc tttcaaacgc gaaggctatc gcaagcgcga cttctcattt agtgacacgc
     3961 tggagatttt gagctcgtcg gggattcgcc gggtgctgca aaaccatcta cgctcaggac
     4021 tgggcgagat gaaaaactcg ctgtgcaaaa gcggctacct gcggctggtg caaaagtatt
     4081 gtccccggct ttcgttaagc gatctccagc cctggcccgc cggtgtgcgg gcgcaggcag
     4141 tatcgccgga cggcaagctg attgacgatt ttctgtttgt caccacccca cgcacgatcc
     4201 acacctgcaa tgcgccctcc ccggcagcga catcagcaat tcctattggt gcgcatattg
     4261 tcagcaaggt acaaacgctg ttggcaagcc agagtaaccc cggacgcacg ctgcgagcgg
     4321 cacgtagtgt ggatgcctta cacgccgcat ttaatcaata acctttgaaa acaggacgta
     4381 acaatgaaac ttaacgacag taacttattc cgccagcagg cgttgattaa cggggaatgg
     4441 ctggacgcca acaatggcgg gatcatcgac gtcaccaatc cggcgaacgg cgacaagctg
     4501 ggtagcgtgc cgaaaatggg cgctgatgaa acccgcgccg ctatcgacgc cgccaaccgc
     4561 gccctgcccg cctggcgtgc gctcaccgcc aaagaacgcg ccaacattct gcgcaactgg
     4621 ttcaatttga tgatggagca tcaggacgat ttagcgcgcc tgatgaccct cgaacagggc
     4681 aaaccactgg ccgaagcgaa aggcgaaatc agctacgctg cctcttttat tgagtggttt
     4741 gccgaagaag gcaaacgtat ttatggcgat accattcccg gtcatcaggc cgataagcgc
     4801 ctgattgtta tcaagcagcc gattggcgtt accgcagcta tcacgccgtg gaacttcccg
     4861 gcggcgatga ttacccgtaa agccggtccg gcgctggcgg caggctgcac catggtgctg
     4921 aagcccgcca gtcagacacc gttctctgcg ctggcgctgg cggagctggc gattcgcgcg
     4981 ggcattccgg ctggcgtgtt taacgtagtc accggttcgg caggcgcggt cggtaatgaa
     5041 ctgaccagca acccgctggt acgcaaactg tcgtttaccg gttcgaccga aattggccgc
     5101 cagttaatgg aacagtgcgc gaaagacatc aaaaaagtgt cgctggagct gggcggcaac
     5161 gcgccgttta tcgtctttga cgatgccgat ctcgataaag ccgtggaagg cgcgctggcc
     5221 tcgaaattcc gcaacgccgg gcaaacctgc gtctgcgcca accgtttata cgtgcaggac
     5281 ggcgtgtatg accgctttgc cgaaaaattg cagcaggcgg tgagcaaact gcacatcggc
     5341 gacgggctgg ataaaggcgt caccatcggg ccgctgatcg atgaaaaagc ggtagcaaaa
     5401 gtggaagagc atattgccga tgcgctggag aaaggtgcgc gtgtggtttg cggcggtaaa
     5461 gcggatgaac gcggcggcaa cttcttccag ccgaccattc tggtggacgt gccggccaac
     5521 gccaaagtgt cgaaagaaga gacgttcggc cccctcgccc cgctgttccg ctttaaagat
     5581 gaagccgatg tgattgcgca agccaatgac accgagtttg gccttgccgc ctatttctac
     5641 gcccgtgatt taagccgcgt cttccgcgtg ggcgaagcgc tggagtacgg catcgtcggc
     5701 atcaataccg gcattatttc caatgaagtg gccccgttcg gcggcatcaa agcctcgggt
     5761 ctgggtcgtg aaggttcgaa gtatggcatc gaagattact tagaaatcaa atatatgtgc
     5821 atcggtcttt aactggagaa tgcgaatgaa cagcaataaa gagttaatgc agcgccgcag
     5881 tcaggcgatt ccccgtggcg ttgggcaaat tcacccgatt ttcgctgacc gcgcggaaaa
     5941 ctgccgggtg tgggacgttg aaggccgtga gtatcttgat ttcgcgggcg ggattgcggt
     6001 gctcaatacc gggcacctgc atccgaaggt ggtggccgca gtggaagcgc agttgaaaaa
     6061 actgtcgcac acctgcttcc aggtgctggc ttacgagccg tatctggagc tgtgcgagat
     6121 tatgaatcag aaggtgccgg gcgatttcgc caagaaaacg ctgctggtta cgaccggttc
     6181 cgaagcggtg gaaaacgcgg taaaaatcgc ccgcgccgcc accaaacgta gcggcaccat
     6241 cgcttttagc ggcgcgtatc acgggcgcac gcattacacg ctggcgctga ccggcaaggt
     6301 gaatccgtac tctgcgggca tggggctgat gccgggtcat gtttatcgcg cgctttatcc
     6361 ttgcccgctg cacggcataa gcgaggatga cgctatcgcc agcatccacc ggatcttcaa
     6421 aaatgatgcc gcgccggaag atatcgccgc catcgtgatt gagccggttc agggcgaagg
     6481 cggtttctac gccgcgtcgc cagcctttat gcagcgttta cgcgctctgt gtgacgagca
     6541 cgggatcatg ctgattgccg atgaagtgca gagcggcgcg gggcgtaccg gcacgctgtt
     6601 tgcgatggag cagatgggcg tggcgccgga tcttaccacc tttgcgaaat cgatcgcagg
     6661 cggtttcccg ctggcgggcg tcaccgggcg cgcggaagtg atggacgctg ttgcaccggg
     6721 cggtctgggc ggcacctatg ccggtaaccc gattgcctgc gtggctgcgc tggaagtgtt
     6781 gaaggtgttc gagcatgaaa atctgctgca aaaagccaac gatctggggc agaagctaaa
     6841 agacggattg ctggcgatag ccgaaaaaca cccggagatc ggcgacgtac gcgggctggg
     6901 agcgatgatt gccatcgagc tgtttgaaga tggcgatcac agcaaaccgg acgcaaaact
     6961 caccgccgag atcgtggcac gcgcccgcga taagggcctg attcttctct cctgcggccc
     7021 gtattacaac gtgctgcgca tccttgtacc gctcaccatt gaagacgctc agatccgtca
     7081 gggtctggag atcatcagcc agtgttttgc tgaggcgaag cagtagctat gtccgtttgt
     7141 aagagttaac cgttgctaag ggaatgataa cgatgcagta aaaaataata acgatgaggc
     7201 gaacgacaaa gttaaaaacg actgcggaca gtcccctcgc ccctctggag agagggttag
     7261 ggtgaggggt aaaacacgcg gctaaaccct cccctggccc tctccctaag ggagagggag
     7321 aaaaccctcc cgctttcgtt tttaagattt tgttgaccgt ctcaattaaa cgcgtgaccc
     7381 ggcgcgggaa atgtcggggc gctctcccaa gtgacacact ttcgagagga ttcaggatgg
     7441 ggcaatcatc gcaaccacat gagttaggcg gcgggctgaa atcacgccac gtcaccatgt
     7501 tgtctattgc cggtgttatc ggcgcaagtc tgtttgtcgg ttccagcgtc gccatcgccg
     7561 aagcgggccc ggcggtatta ctggcctatc tgttcgccgg gctactggtg gttatgatta
     7621 tgcggatgtt ggcggaaatg gcggttgcca cgcccgatac cggttcgttt tccacctatg
     7681 ccgataaagc cattggtcgc tgggcgggct ataccatcgg ctggttgtac tggtggtttt
     7741 gggtactggt tatcccgctg gaagccaata tcgccgctat gatcctgcac tcgtgggttc
     7801 caggcattcc catctggtta ttttccctcg tcattaccct tgccttaact ggcagtaatt
     7861 tattaagcgt taaaaactac ggcgaatttg agttctggct ggcgctgtgc aaagtcatcg
     7921 ctatcctggc ctttattttc cttggtgcag tcgcaattag cggtttttac ccttatgccg
     7981 aagtgagcgg gatctcaaga ttgtgggata gcggcggctt tatgcccaac ggtttcggtg
     8041 cggtattaag cgcgatgttg atcaccatgt tctcgtttat gggcgcagaa attgtcacca
     8101 ttgccgccgc ggaatccgac acgccggaaa aacatattgt ccgcgccacc aactcggtta
     8161 tctggcgtat ttctatcttc tatttgtgct ctatttttgt cgtcgtggca ttaataccgt
     8221 ggaatatgcc ggggctgaaa gccgttggat cttatcgctc ggttctggaa ttgctcaata
     8281 ttccccatgc gaaattaatc atggactgtg tgatattact ttccgtaacc agttgcctga
     8341 actcggccct gtataccgcc tcaaggatgc tctactcctt aagccgtcgc ggtgatgctc
     8401 ccgcggtaat gggtaaaatc aaccgcagta aaaccccgta cgtggcggtg ttactctcca
     8461 ccggcgcggc attcttaacg gtggtggtga actattacgc gcctgcgaag gtatttaaat
     8521 ttctgatcga cagctccggc gctatcgccc tgctggttta tttagtcatc gccgtttcac
     8581 agttgcggat gcgcaaaatt ctgcgagcgg aaggaagcga aattcgcttg cgaatgtggc
     8641 tttatccctg gctcacctgg ctggtcatcg gctttattac ctttgtgttg gtagtgatgc
     8701 tattccgtcc ggcgcaacag ttagaagtga tctccaccgg cttattagcg atagggatta
     8761 tctgtaccgt gccaattatg gctcgctgga aaaagctggt attgtggcaa aaaacacccg
     8821 ttcataatac gcgctgataa tgatcaggag tcacaccatg accattacgt ctctggatgg
     8881 ctatcgctgg ctgaagaacg atattattcg cggtaatttt caaccggatg aaaaattacg
     8941 gatgagtttg ctgacatcgc gttatgcact tggcgttggg ccgttgcggg aagcactttc
     9001 tcaactggtg gcggaacggc tggtcacggt ggtgaatcaa aaagggtatc gggtggcgtc
     9061 tatgtcagag caggagctgc tcgatatttt cgatgcccgc gccaatatgg aagcgatgtt
     9121 agtgagtctg gcgattgccc gcggtggcga tgagtgggag gcagacgttc tcgcaaaagc
     9181 gcatctgctc agtaagctgg aagcctgtga cgccagcgag aaaatgcttg atgagtggga
     9241 tctgcgtcat caggcgtttc atacagcaat tgtggcgggc tgtggttctc actatttgct
     9301 gcaaatgcgt gaacggttgt ttgatctggc ggcgcgctat cgatttatct ggctgcggcg
     9361 aacggtgctt tcggtagaaa tgctggagga taaacacgat cagcaccaga ccctgactgc
     9421 ggcggtactg gcgcgagata ccgcgcgcgc cagtgagtta atgcgccagc atttactgac
     9481 gccaattccc attatccagc aggcgatggc tggcaattaa ctactcttcc ggaatacgca
     9541 acacttgccc cggataaatt ttatccgggc tttttagcat cggtttattc gcttcgaaga
     9601 ttttattgta cagattagcg ttaccgtaga cctgtttgga aatggcactc agagtgtcgc
     9661 cagacttaac ggtataaaac tggctggcag tggctggtgt cgccgttttc acctgatcat
     9721 caacactggc aataccggaa atattcccca ccgcaacaag gattttctcc ttcgcctcct
     9781 gactcaggcc gtcaccagtg accgtcgctt tgccgtcggc aatttgaatg ttcactttat
     9841 cagcatcagg tataccggtt ttgctcagat gctcctgcac cttcttcgcc tgatcgtctt
     9901 tatcgtgctg acctgtaacc gcgtcccaga gtttttctcc ggcatctttc acaaaattga
     9961 acagacccat agctacctca ttcgttaacg gaaacactca gaaagtgtag cagaggaacg
    10021 cggcgcgtta acgccgcggg ggtgagcaca ggtttaatcg cgggtttgca cccagaacgc
    10081 gtgaatcaaa ccggggatat acccaagcag tgtcagcaga atattaataa tgaacgccca
    10141 accgaaccct ttaccgagca gcacgccgag cggcggtaga atgatggtga tgacgattct
    10201 ccagaaaccc atatgtactc cctataagaa aattactcat tgtttaaaaa gagattttat
    10261 ctcttaagcg tagtaatttt agcggagcct gccagtttca gcttacgctt ttactctcat
    10321 ttacgctgct gaggctggta tatgttgcat ttttagttta gagcttactt aatttagaaa
    10381 atacttaaat aaatatgact gaactcgcgc aattacaggc cagtgccgaa caggcagcgg
    10441 ccttattgaa agcaatgagc catcctaaac gattgctgat tctgtgcatg cttagcggtt
    10501 cccccggcac cagcgcggga gagctgacgc gcattaccgg actgagtgcc tctgcgacat
    10561 cacagcatct cgctcgtatg cgggacgaag ggcttatcga cagccaacgg gatgcccaac
    10621 gcattctata ttccattaaa aatgaggcgg taaatgccat tatcgccacc ctgaaaaatg
    10681 tctattgtcc gtaaggagtc actatggctt tgacaaccat ttcgccgcat gatgcacaag
    10741 aattaatcgc acgcggcgca aagttaatcg atattcgtga cgctgatgaa tatcttcgtg
    10801 aacatattcc tgaagcagat ctggctccat tatccgtgct ggaacagtca ggtcttccac
    10861 ctaaattacg tcgcgagcaa attatattcc actgccaggc aggtaaacgc accagtaata
    10921 acgccgataa attagcagcg attgccgccc ccgcagaaat atttttactc gaagatggga
    10981 ttgatggctg gaaaagagct ggattgccag tagcggtaaa taaatctcaa cccttgccgt
    11041 taatgcgcca ggtgcagatc gctgcgggtg gtttaatatt aatcggcgtt gtactgggtt
    11101 ataccgtaaa tagcggtttc ttcttattaa gtggctttgt tggtgccggg ttactgtttg
    11161 caggaatcag cggtttttgt ggaatggcaa ggttgttaga taagatgccg tggaaccaac
    11221 gagcttgaga agcgacgccg gacgcg
//
LOCUS       DAZPDU010000142        11244 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZPDU010000142 DAZPDU010000000
VERSION     DAZPDU010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11244
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.144"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..311
                     /locus_tag="ACHURS_003332"
     CDS             <1..311
                     /locus_tag="ACHURS_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFR1238984.1"
                     /translation="VVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINDLQF
                     FDQQIRWAKADLVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN
                     "
     gene            510..791
                     /gene="ppiC"
                     /locus_tag="ACHURS_003333"
     CDS             510..791
                     /gene="ppiC"
                     /locus_tag="ACHURS_003333"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFR1238985.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(878..2353)
                     /gene="ilvC"
                     /locus_tag="ACHURS_003334"
     CDS             complement(878..2353)
                     /gene="ilvC"
                     /locus_tag="ACHURS_003334"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFR1238986.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRCHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            2503..3396
                     /gene="ilvY"
                     /locus_tag="ACHURS_003335"
     CDS             2503..3396
                     /gene="ilvY"
                     /locus_tag="ACHURS_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFR1238987.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(3448..4992)
                     /gene="ilvA"
                     /locus_tag="ACHURS_003336"
     CDS             complement(3448..4992)
                     /gene="ilvA"
                     /locus_tag="ACHURS_003336"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFR1238988.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(4995..6845)
                     /gene="ilvD"
                     /locus_tag="ACHURS_003337"
     CDS             complement(4995..6845)
                     /gene="ilvD"
                     /locus_tag="ACHURS_003337"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFR1238989.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSEQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(6910..7839)
                     /gene="ilvE"
                     /locus_tag="ACHURS_003338"
     CDS             complement(6910..7839)
                     /gene="ilvE"
                     /locus_tag="ACHURS_003338"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFR1238990.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKCIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(7859..8122)
                     /gene="ilvM"
                     /locus_tag="ACHURS_003339"
     CDS             complement(7859..8122)
                     /gene="ilvM"
                     /locus_tag="ACHURS_003339"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFR1238991.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(8119..9765)
                     /gene="ilvG"
                     /locus_tag="ACHURS_003340"
     CDS             complement(8119..9765)
                     /gene="ilvG"
                     /locus_tag="ACHURS_003340"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFR1238992.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVACSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGIKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(9768..9818)
                     /gene="ilvX"
                     /locus_tag="ACHURS_003341"
     CDS             complement(9768..9818)
                     /gene="ilvX"
                     /locus_tag="ACHURS_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFR1238993.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(9905..10003)
                     /gene="ilvL"
                     /locus_tag="ACHURS_003342"
     CDS             complement(9905..10003)
                     /gene="ilvL"
                     /locus_tag="ACHURS_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFR1238994.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            10356..>11244
                     /locus_tag="ACHURS_003343"
     CDS             10356..>11244
                     /locus_tag="ACHURS_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFR1238995.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGRHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNG"
BASE COUNT         2554 a         3090 c         2951 g         2649 t
ORIGIN      
        1 ttgtcgtgct caacggaaaa attaatggca acgcatgtat cgtcgttcca ttatcaacca
       61 ctcgcgatca tgacaagtta aatcgtggaa tgcatgttga aatagccagt aatgttatca
      121 atgacctgca attctttgat cagcagattc gttgggccaa agccgaccta gtacaacaag
      181 tcagtcgaaa ccgattaaac agagcaagga cttacagagg gtatcttaat caatgtttgc
      241 cacatgagtt ggttgcagac atacagaggg cggtcatcaa gtcaatcaat gccatatcgc
      301 tcataaattg acaataaaat aaccatcccc gataatgact ttgtagccca tagaggccct
      361 aagttttctc cccttgaggg acctgcgaag cccggatttt ccgggctttt gccatatatg
      421 taccagcccc tccctgacta acaattacgt tagcatctgc ggatcatgct aaaatcgccg
      481 cctgacaatt attcactcaa ggcacgatca tggcaaaaac agcagcagca ctgcatatcc
      541 ttgtaaaaga agagaaactg gctctggatc ttctcgagca gattaagaac ggtgccgatt
      601 tcggcaagct ggcgaagaaa cactccattt gcccatcagg caaacgcggc ggtgatttag
      661 gtgaattccg ccagggtcag atggttccgg cgtttgataa agttgtgttc tcttgtccgg
      721 tactggagcc aaccggcccg ctgcacaccc agttcggtta ccacatcatt aaggtgctgt
      781 accgtaacta atagcaaggc cttctccagg agaaggcctt gagtgttttc tccctctccc
      841 tgtgggagag ggtcggggtg agggcatcag cgcgcactta acccgcaact gcaatacgtt
      901 tcatatccgt catatagccg cgcagtttct tacctacctg ctcaatcgca tggcagcgaa
      961 tcgcttcgtt cacatcacgc agttgcgcgt tatctaccgc accttccgga atagctttac
     1021 ccagatcgcc cggttgcagc tctgccataa acggtttcag cagcggcaca caagcgtaag
     1081 agaacagata gttaccgtac tcggcggtat cagagataac cacgttcatt tcgtacagac
     1141 gcttacgggc gatggtgttg gcaatcagcg gcagttcgtg cagtgattca tagtaagcag
     1201 attcttcgat gatgccggaa tcaaccatgg tttcaaacgc cagctcaacg cccgctttca
     1261 ccatcgcgat catcagtacg cctttatcga agtactcctg ctcgccgatt ttgccttcat
     1321 actgcggcgc ggtttcgaag gcggttttgc cggtctcttc acgccaggtc agcagtttct
     1381 tatcatcgtt ggcccagtcc gccatcatac cggaggagaa ttcgccggag atgatgtcgt
     1441 ccatatgttt ctggaacagg ggtgccatga tctctttcag ctgttcagac agcgcgtaag
     1501 cacgcagttt cgccgggtta gagagacggt ccatcatcag ggtgatgccg ccctgcttca
     1561 gcgcttcggt gatggtttcc caaccgaact gaattagttt ttctgcgtat gccggatcgg
     1621 tgccctcttc caccagcttg tcgaagcaca gcagagagcc agcctgcaac ataccgcaca
     1681 ggatggtttg ctcacccatc aggtcagatt tcacttccgc aacgaaggac gattccagca
     1741 cgcccgcacg gtgaccaccg gttgctgccg cccatgcttt agcgatcgcc atgccttcgc
     1801 ctttcggatc gttttccggg tgaacggcaa tcagcgtcgg tacgccgaat ccacgtttgt
     1861 actcttcacg tacttcggtg ccagggcatt tcggcgcaac catgactacg gtaatgtctt
     1921 tacggatctg ctcgcccact tcgacgatgt tgaaaccgtg cgagtaaccc agcgccgcgc
     1981 cgtctttcat cagtggctgt acggtgcgca ctacatcaga gtgctgcttg tctggcgtca
     2041 ggttaaccac cagatccgcc tgcgggatca gttcttcgta agtaccaact ttaaaaccgt
     2101 tttcggtcgc tttacgccag gacgcgcgct tctcggcaat cgcttcttta cgcagagcgt
     2161 aggagatatc gagaccagaa tcacgcatgt tcaggccctg gttcagcccc tgtgcgccac
     2221 agccgacgat aaccactttt ttaccctgaa ggtagctcgc gccgtcggcg aattcgtcgc
     2281 gccccataaa gcgacatttg cccagctgtg ccagctgctg gcgcaggttc agtgtattga
     2341 agtagttagc catggtgatt cctcgtgatg ttgtgcttct tattgttcgg ttcgcgtttg
     2401 cgagattgaa ttcactatat gacaggaaat ttattgcgga aattgatata ttcacaacgt
     2461 cacattgcaa tttctgcaac gtcaacatcg agggctgcgc cattggattt acgcgatctg
     2521 aaaaccttcc tgcatctggc ggaaagccgc cattttggcc gcagcgcgcg ggcgatgcac
     2581 gttagcccat ccacgctctc acggcagatt cagcgcctgg aagaagatct tggtcagccg
     2641 ctatttgtgc gcgataaccg cacggtaacg ctgacggaag cgggcgaaga gctgcgcgtt
     2701 ttcgcccagc aaacgctgtt gcagtatcag caattgcgcc acaccatcga tcagcaaggg
     2761 ccgtcgctct ctggcgaatt acatatcttc tgctcggtga ccgctgccta cagccatctg
     2821 ccgccgattc tggatcgctt ccgcgcggaa cacccgtcgg tggagatcaa actcaccact
     2881 ggtgatgcgg cagatgcaat ggaaaaagtg gtcacgggag aagcggatct ggcgattgcc
     2941 gggaaaccgg agacgctacc cggcgctgtg gcgttttcga tgctggagaa tctggcagta
     3001 gtgctgattg cccccgcgct gccctgcccg gtgcgtaatc aggtatctgt cgaaaagccc
     3061 gactggtcaa cggtgccgtt tattatggcc gatcaggggc cggtacgccg ccgcattgaa
     3121 ctgtggtttc gacgcaataa aatcagcaac ccgatgattt acgccacggt tggcgggcat
     3181 gaagcgatgg tatcgatggt ggcgctcggc tgtggtgtgg cgctgttgcc ggaagtggtg
     3241 ctggaaaaca gcccggagcc tgtacgcaac cgggtaatga ttttagatcg cagcgatgag
     3301 aaaacgccgt ttgaactggg cgtgtgtgca caaaaaaagc ggctgcatga gccgctgatt
     3361 gaggcgttct ggaggatttt gccgaaccac aaatgacgtt gtcgcgcggg taggcctgat
     3421 aagcgaagcg ctatcaggca tttttcccta acccgccaaa aagaacctga acgccggatt
     3481 attggtttcg tcgtggcaat cgtagcccag ctcatttagc cgggtttcga aatccggttc
     3541 atggtcgccg agttcgaacg ccgccagtac gcgcccgtag tcggtgccat ggctgcgata
     3601 gtggaacaaa gaaatgttcc agtgcgtacc cagcgtgttg aggaagcgca gcagcgcgcc
     3661 cggtgattcc gggaattcga agctgtagag gcgttcctgc aacggatgtg acggacgccc
     3721 gccgaccata tagcgcacat gcaacttcgc catttcgtcg tcggagagat ccaccacgct
     3781 gtagccgccg tcgttgagca tctgcaaaat ttctttgcgc tcttcgaggc cgcggctcag
     3841 gcgcacgccg acaaagatgc aggcgttttt ggcatcggca aaacggtagt tgaactcggt
     3901 gaccgatcgc ccgccaagca gttggcagaa tttgaggaag ctgccttttt cttccggaat
     3961 ggtcaccgcc aacaacgctt cacgctgttc gcccagttcg cagcgttccg agacgtagcg
     4021 caggccgtgg aagttcacgt tggcaccgga aagaatatgc gccaggcgtt cgccgcgaat
     4081 gttgtgctga gcgatatatt ttttcattcc cgccagcgcc aacgcgccag agggttccgc
     4141 cactgcgcgc acatcttcga ataaatcctt catcgccgca cagatcgcat cactatcgac
     4201 ggtgatgatg tcgtcgagat actcctggca taaacggaag gtttcgtcgc cgatgcgttt
     4261 taccgcgaca ccttcggcga acagtcccac gcgcggcaga tcaaccggat gacccgcatc
     4321 cagcgctgct ttcaggcagg cggagtcttc cgcttctacg gcgatcactt tgatttgtgg
     4381 catcagttgt ttgatcagca ccgcaacgcc cgccgccaga ccgccgccgc cgactggcac
     4441 aaatacgcgg tcgagatggg cgtcctgctg gagcagttcc agcgccagcg tgccttgccc
     4501 ggcaatcacc atcggatggt cgaacggcgg cacccaggtg aacccctgct gctgtgacag
     4561 ttcgatcgct ttagctttcg cttcgtcaaa gttcgcgcca tgaagcagca cttcgccgcc
     4621 gaatccacgt accgcatcga ctttgatgtc ggcggtggcg gttggcataa cgatcagggc
     4681 cttcacgcct aaccgcgcag aagaaaacgc gacgccctgc gcgtggttac ccgcagaagc
     4741 agtgatcacg ccgtgcgctt tttgttcttc cgtcaggccc gccatcatgg cgtatgcgcc
     4801 gcgcagctta aagctgtgca ctggctggcg atcttcgcgc ttcaccagaa tgacgttatc
     4861 aagacgcgac gacagttttt ccattttttg tagcggcgta acctgcgccg cctcgtaaac
     4921 cggcgcgcgc agcactgctc ttaaatattc ggcaccttcc ggagcaccgg acaggggttg
     4981 cgagtcagcc attattaacc ccccagtttc gatttatcgc gcaccgcacc tttgtcggcg
     5041 ctggttgcca ggctggcgta ggcgcgcagg gcaaaggaaa cctgacgttc acggtttttc
     5101 ggcgtccagg ctttgtcacc ccgagcttcc tgcgcttcac gacgcgccgc cagttcggca
     5161 tcgcttacct gtaactgaat gccacggttc gggatgtcga tagcgatcag gtcaccatct
     5221 tcaatcagac caatgctgcc gccgcttgcc gcttccggtg agacgtggcc gatggaaaga
     5281 ccagaggtgc cgccagagaa acgaccgtcg gtgatcagcg cacaggcttt accgagaccc
     5341 attgatttca ggaagctggt tgggtagagc atttcctgca tgccagggcc gcctttcggg
     5401 ccttcatagc ggatcaccac cacatcgccc gcgacaactt tgccaccgag gatcgcttct
     5461 accgcgtcgt cctggctttc gtacactttc gccgggccgg tgaatttgag gatgctgtca
     5521 tcgacgcccg ctgttttcac gatgcagccg ttttcagcaa aattaccgta gagcaccgcc
     5581 aggccgccgt ctttgctgta ggcgtgttcc agcgagcgga tacagccgtt tgcgcgatcg
     5641 tcatcgagag aatcccaacg gcaatcctgc gagaatgcct gtgtagtacg aatgcccgca
     5701 gggcctgcgc ggaacatatt ttttaccgcg tcatcctggg tcagcataac gtcgtattgt
     5761 tccagagttt gcggcaacgt caggccaagt acgtttttca catcacggtt cagtaacccc
     5821 gcgcgatcca gttcaccgag aataccgata acaccaccag cacggtgaac atcttccata
     5881 tggtattttt gggtgctcgg cgcaactttg cataactgcg ggactttacg cgaaagtttg
     5941 tcgatatcac tcatggtgaa gtcgatttcc gcttcctgcg ccgccgccag caggtgaagt
     6001 accgtgttgg tagagccgcc catcgcgata tccagcgtca tggcattttc aaacgccgct
     6061 ttgctagcaa tattgcgcgg cagtgcactt tcgtcgtttt gctcgtagta acgtttggtc
     6121 aattcaacaa tgcgtttacc ggcattgagg aacaactgct tacggtccgc gtgggttgcc
     6181 agcagcgagc cgttgcctgg ctgcgacaaa cccagcgctt cggtcaggca gttcattgag
     6241 ttagcggtaa acatcccgga gcaggaaccg caggttgggc aggcggaacg ttcaacctga
     6301 tcgctctggg agtcagaaac tttcgggtct gcgccctgga tcatcgcatc aaccagatcg
     6361 agcttgatga tctgctcgga cagtttggtt ttcccggctt ccatcgggcc gccggaaaca
     6421 aagatcaccg gaatattcag gcgcagggaa gccatcagca tccccggggt gattttgtcg
     6481 cagttagaga tgcagaccat ggcgtcggcg cagtgggcgt tgaccatata ctcaacggaa
     6541 tcagcgatca gttcgcgaga tggcagtgaa taaagcatcc ccccgtggcc catagcaatc
     6601 ccatcatcca ccgcaatggt gttgaactct ttggcaacgc cgccagccgc ttcaatttgt
     6661 tcggcgacca gtttaccgag atcgcgcaga tggacgtgac ccggtacaaa ttgggtgaac
     6721 gagttcacaa ccgcgataat cggcttgcca aaatcggcat cggtcattcc ggtggcgcgc
     6781 cacagcgcac gagcacccgc catattacga ccatgagtgg tggtggcgga acggtactta
     6841 ggcatacttt atttactccc agtgtctgtc tcgtaaatgg gacggtaagt gccgtcccat
     6901 tttttgtatt tattgattaa cttgatctaa ccagccccat ttatcttcgg tttcgccagt
     6961 gaagaggccg aagaaggctt gctgaatgca tttggtaacc gggccgcaac ggccttcacc
     7021 cacctgaata ccgtctacac tgcgcactgg cgtgatttct gccgccgtac cggacataaa
     7081 cacttcatcc gccaggtaca gggattcgcg cgacagcacc tgctcacgca cttcaattcc
     7141 cagctctttc gccagtttga tgatggcatc acgggtaata cccggcagcg cggatgaggt
     7201 aaacggtggg gtgaacagca cgccgtcttt cacttcaaac aggttttcac ctgcgccttc
     7261 agagatgtaa ccgttcacat ccagcgcgat accttcctga taaccgtggc ggcgcgcttc
     7321 gctacccacc agcagggaag agaggtagtt accaccggct tttgccgccg tcgggatggt
     7381 gtttggtgct gcgcggttcc aggaggaaac catcgcatcg atcccctgct ccagcgcttc
     7441 tgcgcccaga tacgctcccc acgggaaagc agcgataatc acgtcggttg agtatcccgc
     7501 tggcgggttt actcccatgc caacatcacc gacgaagatc agcggacgga tataggcgct
     7561 ggtgagatta tttttgcgga tcacgtcacg acaagcttcc atcagctcat caatgctctg
     7621 cgaaaccggg aagcgataga ttttggcgga gtcatgcaga cgctgcatat gctcacgatg
     7681 gcggaataca accggtcctt tgtgcgagtc gtagcaacgg atgccttcaa aaaccgaagt
     7741 gccatagtgc agcgcgtgcg acatcacatg caccttcgcg tcttcccagc gaaccatctc
     7801 cccattgaac caaatgtaat cagctttctt cgtggtcatt tttatattcc ttttgcgctc
     7861 aggcgcggat ttgttgtgat gtggttgtgc tctggcagat ggcaacgtgt gcgacgtcca
     7921 ccagtttatt taactgacta aacagtaagt cgaccgaccg tgggctggca acggtcaatt
     7981 cgatatttat attttgtgca tcgctggcgg cggccatatt cattgagcag acgtggaaac
     8041 cacgatgacg caccacgcgt aaaacacgtt ctaaggtttc tggattgaag cgagccgata
     8101 cattgacctg atgttgcatc atgataattt ctccaacatt tctgaattac tggcgccagg
     8161 cggcaccagc ggccagacgt tctcaagttc gtcgattgag acatgaagca ggtatggccc
     8221 atcactgttc agcatggtgt cgagtgccgc ttcaacctgg tctttacggg tgatgtgttg
     8281 gccagggatg ccgaaggcgc tggctaacat gaggaaatcg gggttatcag taagggtggt
     8341 ttcgctgtat cgttcctgaa aaaacagttg ctgccattgt cgaaccatcc ctaaccgttg
     8401 gttatcgagt aagacgattt tcaacggtaa ctgcttgcgt tttacggtgc ccagctcttg
     8461 cacattcatc atgaaagagc cgtcaccgga gatacagaca acggtatcgt tcggtcgcgc
     8521 gacttgtgcg ccaaccgccg ccggtaaacc aaaacccatg gtacctaagc cgctggaggt
     8581 gatgaaattt tccgggcgag tgtgggcgat gtgctgcgca gcccacatct ggtgctgccc
     8641 cacatctgtg gtcacgacgc aatccgcagg tttacgatcc gacagttgtt ttaacaacaa
     8701 cggcgcgtag atagcgtcac cgggatggtc gtaacgccag gaatgttcat cacgcagctg
     8761 cgcgcagtat tgctgccagt cattgatatt taacggctgc tgtaatgctg gtaacagagc
     8821 atttaaatca ccctgtaatg ccacatgtgc ctgacgcagc ttgttcattt ctgccgggtc
     8881 gatatccata tggataacac tggcgtgtgg tgcgaaagta ttcagtttgc cggtcacccg
     8941 gtcatcaaaa cgtgcgccca cggcgatcag caggtcacac tcctgcaccg cgaagtttgc
     9001 cgctttgatg ccgtgcatcc ccagcatgcc cagatagtac ggataatctg cttctactgc
     9061 gcccagccct ttcagcgtac aggtggcagg catttttgtg gcagcgagaa attcacgcaa
     9121 agccggaact gcctgcgcca tacccacgcc accgccaacg tacagcatcg gtttttgcgc
     9181 ttttgccagc atctggcgcg cttgctcaac ttcggcatgt gggaaagtca cttcgttttc
     9241 aacggtggtg aaccacggtt ccaggtcacc gctggctaac tggatatctt ttgggatatc
     9301 gaccagaacc ggaccaggac gacctgagca ggcaacgtcg aatgcttcag ccatgatgcg
     9361 cggcaactct tccagcgact gcaccagaaa gctgtgcttg gtacaggcta acgacaatcc
     9421 taggacatcc acttcctgaa atgcgtcagt gccgataaac ggtgcggaca cttgaccggt
     9481 gatggcaaca acagggatgg aatctaacag tgcgtccgca agcccggtta tcaggttggt
     9541 tgcgcccgga ccagacgtgg cgatacatac gccagttttg ccggtagcac gggcataacc
     9601 gatagccgcc attgccgcac cctgctcatg tcggcatagc aagtgctcca cgccgccgtc
     9661 atacaatgca tcgtaaaccg gcataattgc gccacccgga taaccgaaaa cggtgttcac
     9721 accctgtgcc cgcaacgcat gtaccaccca ctgtgcgcca ttcatagtta gttccccgtc
     9781 ctgaatcttg agaaacagaa ttttgtgctg ttattcattg tctgctcctc ggttatgttt
     9841 ttaaggtcaa aaaaaacccc cggacctttc ggtgcggggg tcttagttcg ttaaggcttg
     9901 atctctaagc ctttcctcgt ccaagtgcag ccccgcacgg tgggataata atcaccacca
     9961 cgctaatcac gactaggcta atcactcgta gaagggctgt cattttgtct tttcttgcat
    10021 cttgttcgaa ggaatgccta aagagttacc atagattttg cgaatagcac aagatatttt
    10081 tgggcctatt tttatgatag ctttaataat ttcaatagaa gttgttgttt tttaaaaaga
    10141 aaatagtgag tgaaaaaaat tcaatttcag tataaatgtt gttcccttcc tcgtagttca
    10201 tcctttgtcc gctgcatttt tgcgagcatc attccaccgc cagaacaact caacttcact
    10261 tgcacaaata aacaggaatc agccactaaa attcacgaat tagcgggcta cagcagcgat
    10321 atcttatgca cataatggtg actcaaggag ggcttatgtc actgtcaatt gttcataccc
    10381 gcgcagccct gggagtaaat gcgcccccga tcactgttga ggtacatatc agtaaaggtc
    10441 tacccggctt aacgatggtg ggcttaccag aaacaactgt aaaagaagct cgcgatcgcg
    10501 tgcgcagcgc cattatcaat agcggatatg aatatccggc gaaaaaaatc accatcaacc
    10561 ttgctccagc cgatctgcca aaagaagggg gacgatatga tttacctatc gccattgcgt
    10621 tgctggcggc ctcagaacag cttacagcca ataagttaga tgaatatgaa ttagtcggag
    10681 aactggctct tacaggcgct ctgcgtggcg ttcccggtgc aatctccagt gcaactgaag
    10741 ctattaagtc gggcagaaaa attatcgtcg cgaaagataa cgaagatgaa gtggggctaa
    10801 ttaacggtga aggatgcctg atagccgatc atctgcaagc tgtctgtgcg tttctggaag
    10861 gtaagcacgc tctcgaacgc ccgaaaccaa ctgatgcagt atcccgggcg ctacaacatg
    10921 atctcagtga tgttgtcggt caggaacaag gaaagcgagg actggaaatt accgccgctg
    10981 gcaggcacaa ccttttactg attgggccgc cgggaacagg taaaacaatg ctcgccagcc
    11041 gtattaatgg cctgttgcca gatttaagca atgaagaggc actggagagc gctgcgatat
    11101 taagtctggt aaatgctgaa tcagtacaaa aacaatggcg gcagcgcccg ttccgctcac
    11161 ctcatcacag tgcgtcgtta actgcgatgg tgggcggtgg tgcaattcca gggccaggtg
    11221 aaatttcgtt ggcgcataac ggcg
//
LOCUS       DAZPDU010000143        11218 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZPDU010000143 DAZPDU010000000
VERSION     DAZPDU010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11218
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.145"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            53..352
                     /locus_tag="ACHURS_003344"
     CDS             53..352
                     /locus_tag="ACHURS_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFR1238996.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMESGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(472..651)
                     /locus_tag="ACHURS_003345"
     CDS             complement(472..651)
                     /locus_tag="ACHURS_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFR1238997.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            725..2086
                     /gene="pabB"
                     /locus_tag="ACHURS_003346"
     CDS             725..2086
                     /gene="pabB"
                     /locus_tag="ACHURS_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFR1238998.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2090..2668
                     /locus_tag="ACHURS_003347"
     CDS             2090..2668
                     /locus_tag="ACHURS_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFR1238999.1"
                     /translation="MEYRSLMLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            2852..4216
                     /gene="sdaA"
                     /locus_tag="ACHURS_003348"
     CDS             2852..4216
                     /gene="sdaA"
                     /locus_tag="ACHURS_003348"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFR1239000.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4347..5945
                     /locus_tag="ACHURS_003349"
     CDS             4347..5945
                     /locus_tag="ACHURS_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFR1239001.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVQFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDMLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(5949..7505)
                     /gene="yoaE"
                     /locus_tag="ACHURS_003350"
     CDS             complement(5949..7505)
                     /gene="yoaE"
                     /locus_tag="ACHURS_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFR1239002.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(7493..7651)
                     /gene="yoaL"
                     /locus_tag="ACHURS_003351"
     CDS             complement(7493..7651)
                     /gene="yoaL"
                     /locus_tag="ACHURS_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFR1239003.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            7968..8939
                     /gene="manX"
                     /locus_tag="ACHURS_003352"
     CDS             7968..8939
                     /gene="manX"
                     /locus_tag="ACHURS_003352"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFR1239004.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            9002..9802
                     /gene="manY"
                     /locus_tag="ACHURS_003353"
     CDS             9002..9802
                     /gene="manY"
                     /locus_tag="ACHURS_003353"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFR1239005.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            9815..10666
                     /gene="manZ"
                     /locus_tag="ACHURS_003354"
     CDS             9815..10666
                     /gene="manZ"
                     /locus_tag="ACHURS_003354"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFR1239006.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10721..11179
                     /locus_tag="ACHURS_003355"
     CDS             10721..11179
                     /locus_tag="ACHURS_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFR1239007.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
BASE COUNT         2757 a         2823 c         2829 g         2809 t
ORIGIN      
        1 taaggtcata tatagcctta tcgataacat ggttaatttt aaggataaga atatgcctgc
       61 tgtaatagat aaagccctgg atttcattgg tgccatggat gtatcagcgc caacaccaag
      121 ttcgatgaat gaaagcacgg cgaagggaat ctttaaatat ttaaaagaac tgggagtacc
      181 tgcgagtgcc gctgatatta cggcgcgagc tgaccaggaa ggctggaacc ctgggtttac
      241 ggaaaaaatg gttggatggg caaaaaaaat ggagtcaggt gaacgctctg tgattaaaaa
      301 tcctgaatac ttttcaacat atatgcagga agaactaaaa gcactggtct gagtaaaatt
      361 tacatcagca taaatggatc aggacgcttt taatcacata taaaaagcgt cctttttctc
      421 acaatcaaat aatcatcggc ttgtataacg aaatcaatac ggattagtgt gttactcgtc
      481 ttcatcctca aaacgcgcca cgattcgctc gccggaatgg ttggcacgca actcttctgc
      541 caccaacgcg attgcctggc cgctgctcat cccttgtgcc atcagttcct ggatccgctc
      601 gacagctttt tgctgctgtt catgggtgag tgaaggtaaa cctgcaaaca ttgttaactc
      661 ctgctaaatt gttggcgcta attatttcat gctacccggc acatagccag tagagtcagg
      721 actgatgaag acgttatctc ccgctgtgat tactttaccc tggcgtcagg acgccgctga
      781 attttatttc tcccgcttaa gccacctgcc gtgggcgatg cttttacact ccggctatgc
      841 cgatcatccg tatagccgct ttgatattgt ggtcgccgat ccgatttgca ctttaaccac
      901 tttaggtaaa gaaaccgttg ttagtgaaag cgaaaaacgc acaacgacca ctgatgaccc
      961 gctacaggtg ctccagcagg tgctggatcg cgcagacatt cgcccaacgc ataacgaaga
     1021 tttgccattt cagggcggcg cactggggtt gtttggctac gatctgggcc gccgttttga
     1081 gtcactgcca gaaattgcgg aacaagatat cgttctgccg gatatggcag tgggtatcta
     1141 cgattgggcg ctcattgtcg accaccagcg tcatacagtt tctttgctga gtcataatga
     1201 tgtcaatgcc cgtcgggcct ggctggaaag ccagcaattc tcgccgcagg aagatttcac
     1261 gcttacttcc gactggcaat ccaatatgac ccgcgagcag tacggcgaaa aatttcgcca
     1321 ggtacaggaa tatctgcaca gcggtgattg ctatcaggta aatctcgccc agcgttttca
     1381 tgcgacctat tctggcgatg aatggcaggc attccttcag cttaatcagg ccaaccgcgc
     1441 gccatttagc gcttttttac gtcttgaaca gggtgcaatt ttaagccttt cgccagagcg
     1501 gtttattctt tgtgataata gtgaaatcca gacccgccct attaaaggca cgctaccacg
     1561 cctgcccgat cctcaggaag atagcaaaca agcagaaaaa ctggcgaact cagcgaaaga
     1621 tcgtgccgaa aatctgatga ttgtcgattt aatgcgtaat gatatcggtc gtgttgccgt
     1681 agcaggttcg gtaaaagtac cagagctgtt cgtggtggaa cccttccctg ccgtgcatca
     1741 tctggtcagc accataacgg cacaactacc agaacagtta cacgccagcg atctgctgcg
     1801 cgcagctttt cctggtggct caataaccgg ggctccgaaa gtacgggcta tggaaattat
     1861 cgacgaactg gaaccgcagc gacgtaatgc ctggtgcggc agcattggct atttgagctt
     1921 ttgcggcaac atggatacca gcattactat ccgcactctg actgccatta acggacaaat
     1981 atactgctct gcgggcggtg gaattgtcgc cgatagccag gaagaagcgg aatatcagga
     2041 aacttttgat aaagttaata agatattacg ccaactggag aagtaagacg tggaataccg
     2101 tagcctgatg cttgatgatt ttttatcgcg ctttcaactt ttgcgcccgc aaattaaccg
     2161 ggaaacccta aatcatcgtc aggctgccgt gttaatcccc atcgtccgtc gaccgcaacc
     2221 ggggttgttg ctgactcagc gttcaattca tctgcgtaaa cacgctggac aagtggcatt
     2281 ccctggaggt gcagtcgatg acacggacgc atcagttatc gccgccgcgc tgcgcgaagc
     2341 tgaagaagag gtcgctatac cgccttccgc cgttgaagtt atcggcgtgc tgccgcccgt
     2401 cgatagcgta actggctatc aggtaacccc agtggtcggc attatcccgc ccgatctgcc
     2461 gtatcgcgcc agtgaagatg aagtctcggc ggtgtttgaa atgccgctcg cccaggcatt
     2521 acatctgggt cgttatcacc ctttagatat ctaccgccgt ggcgattcac atcgggtatg
     2581 gctgtcctgg tacgaacagt attttgtatg gggaatgacc gcaggcataa ttcgtgagct
     2641 ggcgctgcaa attggtgtga aaccctgact atacttatct ttacatctac aaaacactac
     2701 ttgagacaat catcgcaata ttagttaaat cgcggttttt gattagttta attcatgtga
     2761 atagttaagc cagtcgccgc gttccctctt acactatgcg ctgttattag ttcgttactg
     2821 gaagtccagt caccttgtca ggagtattat cgtgattagt ctattcgaca tgtttaaggt
     2881 ggggattggt ccctcatctt cccataccgt agggcctatg aaggcaggta aacagttcgt
     2941 cgatgatctg gtcgaaaaag gcttactgga tagcgttact cgcgttgccg tggacgttta
     3001 tggttcactg tcgctgacgg gtaaaggcca ccacaccgat atcgccatta ttatgggtct
     3061 tgcaggtaac gaacctgcca ccgtggatat cgacagtatt cccggtttta ttcgcgacgt
     3121 agaagagcgc gaacgtctgc tgctggcaca gggacggcat gaagtggatt tcccgcgcga
     3181 caacgggatg cgttttcata acggcaacct gccgctgcat gaaaacggta tgcaaatcca
     3241 cgcctataac ggcgatgaag tcgtctacag caaaacttat tattccatcg gcggcggttt
     3301 tatcgtcgat gaagaacact ttggtcagga tgctgccaac gaagtgagcg tgccgtatcc
     3361 gttcaaatct gccaccgaac tgctcgcgta ctgtaatgaa accggctatt cgctgtctgg
     3421 tctcgctatg cagaacgaac tggcgctgca cagcaagaaa gagatcgacg agtatttcgc
     3481 gcatgtctgg caaaccatgc aggcatgtat cgatcgcggg atgaacaccg aaggtgtact
     3541 gccaggcccg ctgcgcgtgc cacgtcgtgc gtctgccctg cgccggatgt tagtttccag
     3601 cgataaactg tctaacgatc cgatgaatgt cattgactgg gtaaacatgt ttgcgctggc
     3661 agttaacgaa gaaaacgccg ccggtggtcg tgtggtaact gcgccaacca acggtgcctg
     3721 cggtatcgtt ccggcagtgc tggcttacta tgaccacttt attgaatcgg tcagcccgga
     3781 catctatacc cgttacttta tggcagcggg cgcgattggt gcattgtata aaatgaacgc
     3841 ctctatttcc ggtgcggaag ttggttgcca gggcgaagtg ggtgttgcct gttcaatggc
     3901 tgctgcgggt cttgcagaac tgctgggcgg tagcccggaa caggtttgcg tggcggcgga
     3961 aattggcatg gaacacaacc ttggtttaac ctgcgacccg gttgcagggc aggttcaggt
     4021 gccgtgcatt gagcgtaatg ccattgcctc tgtgaaggcg attaacgccg cacggatggc
     4081 tctgcgccgc accagtgcac cgcgcgtctc gctggataag gtcatcgaaa cgatgtacga
     4141 aactggtaag gacatgaacg ccaaataccg cgaaacctca cgcggtggtc tggcaatcaa
     4201 agtccagtgt gactaatact tcttactcgc ccatctgcaa cggatgggcg aatttatacc
     4261 cgctttctcg tctgctgtaa tattccccac tacacttcca ctgttgcgtc aggcgtttgt
     4321 cgccatacgc ttacagggtg gcccgcatgc aaaaagcaca acggatcatt aaaacctatc
     4381 gccgtaatcg aatgattgtt tgtacgattt gcgcactcgt tacgctcgct tcgaccctga
     4441 gcgtgcaatt tatttcacag cgtaacttaa atcaacaacg ggtagtacaa ttcgccaatc
     4501 acgctgtaga ggaattagat aaagtactgc ttcccctaca ggcaggtagc gaagtcttgc
     4561 ttccgctgat tggtctgccc tgctctgtcg cccatttgcc attacgtaaa caggcggcaa
     4621 aactccaaac tgtgcgatcc attggcctgg tgcaagacgg aacactctat tgctccagca
     4681 tttttggtta tcgcaatgtg cccgtcgtgg acattctggc tgaacttcct gcaccgcaac
     4741 cacttttacg cctgacgatc gaccgtgccc tgattaaagg cagtccggtt ttgattcaat
     4801 ggacgcctgc agcgggcagt agcaatgctg gggtcatgga gatgattaac atcgacttac
     4861 tgacggcaat gctccttgag ccacaactgc cgcaaatcag tagcgccagc ctgacggtgg
     4921 acaaacggca tttgctctat ggtaatgggc tggtagattc ccttccgcaa cctgaagaca
     4981 atgaaaacta tcaggtttct tcgcaacgct ttccttttac cattaacgtt aatggtccgg
     5041 gggctacggc gctggcatgg cactatcttc caacacaatt accgctggcg gtgctgctaa
     5101 gtttactggt gggctacatc gcctggctgg cgaccgctta ccggatgagc ttttcccgcg
     5161 aaatcaatct gggcctggcg caacatgagt tcgaattgtt ctgtcagcct ttgcttaatg
     5221 cgcgcagcca gcaatgtatt ggtgtagaga ttttgctgcg ctggaacaat ccgcgtcagg
     5281 gctggatttc accggatgtg tttattccta tcgcggaaga acatcattta attgtgccac
     5341 tgacccgcta tgtgatggca gaaaccattc gtcagcgcca tgttttcccg atgagtagtc
     5401 agtttcatgt tggcattaac gtcgcaccca gccattttcg ccgtggtgtg ctgataaaag
     5461 atctcaatca gtactggttt agcgctcacc cgattcagca actgatcctc gaaatcaccg
     5521 aacgcgatgc cttactggat gttgattatc ggattgcccg cgagctacat cgtaaaaacg
     5581 tcaaactggc gattgatgac ttcggcaccg gcaacagttc attttcctgg cttgaaacat
     5641 tacgtcctga catgctgaaa attgataagt catttaccgc agctataggt tctgacgcgg
     5701 ttaactcgac ggtgaccgat atcatcatcg cgctggggca aagactgaat attgaactgg
     5761 tggcggaggg cgtggaaacg caagaacagg cgaagtattt gcgccgtcat ggcgtgcata
     5821 ttttgcaagg gtatttgtac gcacagccga tgccgctacg tgattttccc aaatggctgg
     5881 cgggcagcca accgccgccc gcccggcata atggacatat cacgcccatt atgccgttac
     5941 gttaatgctt actcatcttc atcgtgcgcc ggttgctctt taacaatgcg aaccagatca
     6001 acacgataat cattggcttc aatgatggtg atatgcagtg gccctacatc aatcacatcg
     6061 cccacacggg gaatgtgacc atttgccgag atcacgaggc ccgcgaccgt cgcgatatca
     6121 tcgtcatcgg caaggtgctc aacatcaagc gcctgctgca aggcatgcaa atctgtaccg
     6181 ccttttacca gccagccgtc accatcagta atgatttccg gcgtttcgtc agcgtccggg
     6241 aattcacccg caatggcttc cagcacatcc agcggcgtga ccagaccttg taccacacca
     6301 aactcgttgg tcacgataac aaagctcccg cgagcacgac gcagcacgcc caacaggttg
     6361 atcggatcga gggtttccgg gacgataatc gccggagacg ccgaagcaat cgccgccaca
     6421 tcaacgccct cttccagcgc caccagcagt tctttagcac gtacaatacc gatgatttca
     6481 tccagttcac cgcgacatac cgggaacaga ctgtgcggtg aagagagcag ttgctcgcgg
     6541 atttcatcga ccccgagatt agcgtcaacc cagcttattt caccgcgcgg cgtcatgatc
     6601 ccgcgcagag aacgcgacgc aagcgtcagt acgccgttaa tcatgtaacg ttcttcttcg
     6661 gcaaatgcac cttccgggat cggcatcggc atcgggttat cggcatcgtg ctgaacattg
     6721 gcctgacgtt tcccgcccat caaacgcagg atggcatcgg cagtacgcgc tcgcagcggc
     6781 aaagtcgact ggtggcgaat aaagttgcga cgcgcaatct ggttaaacac ttcgatgatg
     6841 atcgagaagc caatcgcggc atacaggtaa cctttcggaa tgtggaaacc gaaaccttct
     6901 gccaccagac tcagaccaat cattaacagg aagctcagac agagcaccac caccgtgggg
     6961 tgctggttaa cgaatcgcgt cagcggtttg gatgccagca acataaccgc catcgcaatc
     7021 actaccgccg ccatcatcac cggcagatgg ttaaccatcc ctactgcagt aattaccgca
     7081 tccaacgaga agacggcgtc aaggatgaca atctgtgtga cgaccaccca gaaactggcg
     7141 tagcctttac cgtggccgga atcatgatcg cggttttcca gccgttcatg cagttcggtt
     7201 gttgctttga acagcaagaa tatccccccg aacaacataa tcaggtcgcg tccggagaag
     7261 gagaaatcca tgacggtaaa tagcggtttg gtcagcgtga ccatccatga aatcagcgac
     7321 agcagcccca gacgcataat cagcgccagt gataacccca gcaaacgcgc tttatcgcgt
     7381 tgttttggcg gcagtttgtc agcaagaatg gcgatgaaga ccaggttatc gatacccagc
     7441 acaatttcga gaacaacaag cgtgagtagc cccgcccaaa ttgaggggtc cattaagaat
     7501 tccatgacaa gctcctgctt aaggaatagc tattcgacgc cagaaataat gcaggcgtaa
     7561 cgacaaaatg caaacgaaag gtgcggcata gagtgccaga aaggcaggcg ttaaaaggcc
     7621 tgatgctgaa atgacgtcgg tgacgatcca tactgcgggc tactgcccta tactccatgg
     7681 ttgttaaacg ggagttaaac atatcagaga cgcctctgat ttggcaaaga tttaccttcc
     7741 tttgcaaacg aatgtgacaa ggatatttta cctttcgaaa tttctgctaa tcgaaagtta
     7801 aattacggat cttcatcaca taaaataatt ttttcgatat ctaaaataaa tcgcgaaacg
     7861 caggggtttt tggttgtagc ccttatctga atcgattcga ttgtggacga cgattcaaaa
     7921 atacatctgg cacgttgagg tgttaacgat aataaaggag gtagcaagtg accattgcta
     7981 ttgttatagg cacacatggt tgggctgcag agcagttgct taaaacggca gaaatgctgt
     8041 taggcgagca ggaaaacgtc ggctggatcg atttcgttcc aggtgaaaat gccgaaacgc
     8101 tgattgaaaa gtacaacgct cagttggcaa aactcgacac cactaaaggc gtgctgtttc
     8161 tcgttgatac atggggaggc agcccgttca atgctgccag ccgcattgtc gtcgacaaag
     8221 agcattatga agtcattgca ggcgttaaca ttccaatgct cgtggaaacg ttaatggctc
     8281 gtgatgatga cccaagcttt gatgaactgg ttgcgctggc agtagaaaca ggccgtgaag
     8341 gcgtgaaagc actgaaagcc aaaccggttg aaaaagccgc gccagcaccc gctgccgcag
     8401 caccaaaagc ggctccaact ccggcaaaac caatggggcc aaacgactac atggttattg
     8461 gccttgcgcg tatcgacgac cgtctgattc acggtcaggt cgccacccgc tggaccaaag
     8521 aaaccaatgt ctcccgtatt attgttgtta gtgatgaagt ggctgcggat accgttcgta
     8581 agacactgct cacccaggtt gcacctccgg gcgtaacagc acacgtagtt gatgttgcca
     8641 aaatgattcg cgtctacaac aacccgaaat atgctggcga acgcgtaatg ctgttattta
     8701 ccaacccaac agatgtagag cgtctcgttg aaggcggcgt gaaaatcacc tctgttaacg
     8761 tcggtggtat ggcattccgt cagggtaaaa cccaggtgaa taacgcggtt tcggttgatg
     8821 aaaaagatat tgaggcgttc aagaaactga atgcgcgcgg tattgagctg gaagtccgta
     8881 aggtttccac cgatccgaaa ctgaaaatga tggatctgat cagcaaaatc gataagtaac
     8941 gtattgtgtt gattatcact cagttttcac acttaagtct tacgtaaaca ggagaagtac
     9001 aatggagatt accactcttc aaattgtgct ggtatttatc gtagcctgta tcgcaggtat
     9061 gggatcaatc ctcgatgaat ttcagtttca ccgtccgcta atcgcgtgta ccctggtggg
     9121 tatcgttctt ggggatatga aaaccggtat tattatcggt ggtacgctgg aaatgatcgc
     9181 gctgggctgg atgaacatcg gtgctgcagt tgcgcctgac gccgctctgg cttctatcat
     9241 ttctaccatt ctggttatcg caggtcatca gagcattggt gcaggtatcg cactggcaat
     9301 ccctctggcc gctgcgggcc aggtactgac catcatcgtt cgtactatta ccgttgcttt
     9361 ccagcacgct gcggataagg ctgctgataa cggcaacctg acagcgattt cctggatcca
     9421 cgtttcttct ctgttcctgc aagcaatgcg tgtggctatt ccggccgtca tcgttgcgct
     9481 gtctgttggt accagcgaag tacagaacat gctgaatgcg attccggaag tggtgaccaa
     9541 tggtctgaat atcgccggtg gcatgatcgt ggtggttggt tatgcgatgg ttatcaacat
     9601 gatgcgtgct ggctacctga tgccgttctt ctacctcggc ttcgtaaccg cagcattcac
     9661 caactttaac ctggttgctc tgggtgtgat tggtactgtt atggcagtgc tctacatcca
     9721 acttagcccg aaatacaacc gcgtagccgg tgcgcctgct caggcagctg gtaacaacga
     9781 tctcgataac gaactggact aacaggtgag cgaaatggtt gatacaactc aaactaccac
     9841 cgagaaaaaa ctcactcaaa gtgatattcg tggcgtcttc ctgcgttcta acctcttcca
     9901 gggttcatgg aacttcgaac gtatgcaggc actgggtttc tgcttctcta tggtaccggc
     9961 aattcgtcgc ctctaccctg agaacaacga agctcgtaaa caagctattc gccgtcacct
    10021 ggagttcttt aacacccagc cgttcgtggc tgcgccgatt ctcggcgtaa ccctggcgct
    10081 ggaagaacag cgtgctaatg gcgcagagat cgacgacggt gctatcaacg gtatcaaagt
    10141 cggtttgatg gggccgctgg ctggtgtagg cgacccgatc ttctggggaa ccgtacgtcc
    10201 ggtatttgca gcactgggtg ccggtatcgc gatgagcggc agcctgttag gtccgctgct
    10261 gttcttcatc ctgtttaacc tggtgcgtct ggcaacccgt tactacggcg tagcgtatgg
    10321 ttactccaaa ggtatcgata tcgttaaaga tatgggtggt ggcttcctgc aaaaactgac
    10381 ggaaggggcg tctatcctcg gcctgtttgt catgggggca ttggttaaca agtggacaca
    10441 tgtcaacatc ccgctggttg tctctcgcat tactgaccag acgggcaaag aacacgttac
    10501 tactgtccag actattctgg accagttaat gccaggcctg gtaccactgc tgctgacctt
    10561 tgcttgtatg tggctactgc gcaaaaaagt taacccgctg tggatcatcg ttggcttctt
    10621 cgtcatcggt atcgctggtt acgcttgcgg cctgctggga ctgtaagact gttgtacact
    10681 accggggcct tttggccccg tttttttatc tggaggatta atgacaatca cggacctggt
    10741 actgattctt ttcatcgccg cactcctggc cttcgcgatc tacgatcagt tcatcatgcc
    10801 ccgccgtaac ggccccaccc tgctggcaat tcctttgctc cggcgtggtc gcatcgatag
    10861 cgttatcttc gtcggattga ttgtcattct tatctataac aacgtcacga atcatggtgc
    10921 gttaataacg acatggttat taagcgcact ggctctgatg ggtttttata tattctggat
    10981 ccgcgttccg aagatcatct ttaaacaaaa aggttttttc ttcgccaatg tctggattga
    11041 atatagccga atcaaagcga tgaacttgtc ggaagatggc gtgttggtga tgcaattaga
    11101 acagcgtcgg ctgttaatcc gcgttcgaaa tatcgacgat ctggaaaaaa tttataagct
    11161 tctcgtttca actcaataag ttatgaattt agccaaagct atgtttagtg tattttta
//
LOCUS       DAZPDU010000144        11144 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZPDU010000144 DAZPDU010000000
VERSION     DAZPDU010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11144
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.146"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..437
                     /locus_tag="ACHURS_003356"
     CDS             <1..437
                     /locus_tag="ACHURS_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR1239008.1"
                     /translation="KCLFHDCSMHGVKIKPWLPVKWTKELINDYLYGCLLSLYSICAR
                     DIYNMSAGNNVKVAADAFLEIIFSLKNKYCIKLLSAQERAFIYEFARMIFAYINDKSI
                     EILLLSCFAASDQKAIQRYIPQSQDGEDFRSHLQYKLPLSAH"
     gene            648..971
                     /locus_tag="ACHURS_003357"
     CDS             648..971
                     /locus_tag="ACHURS_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFR1239009.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            968..1798
                     /gene="amiD"
                     /locus_tag="ACHURS_003358"
     CDS             968..1798
                     /gene="amiD"
                     /locus_tag="ACHURS_003358"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFR1239010.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPTQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(1795..2808)
                     /locus_tag="ACHURS_003359"
     CDS             complement(1795..2808)
                     /locus_tag="ACHURS_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFR1239011.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENTVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPIITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(2907..4337)
                     /locus_tag="ACHURS_003360"
     CDS             complement(2907..4337)
                     /locus_tag="ACHURS_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFR1239012.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKASGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(4348..5349)
                     /gene="ltaE"
                     /locus_tag="ACHURS_003361"
     CDS             complement(4348..5349)
                     /gene="ltaE"
                     /locus_tag="ACHURS_003361"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFR1239013.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRKRN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(5386..7104)
                     /gene="poxB"
                     /locus_tag="ACHURS_003362"
     CDS             complement(5386..7104)
                     /gene="poxB"
                     /locus_tag="ACHURS_003362"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFR1239014.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(7237..8205)
                     /gene="hcr"
                     /locus_tag="ACHURS_003363"
     CDS             complement(7237..8205)
                     /gene="hcr"
                     /locus_tag="ACHURS_003363"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFR1239015.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(8217..9869)
                     /gene="hcp"
                     /locus_tag="ACHURS_003364"
                     /gene_synonym="priS"
     CDS             complement(8217..9869)
                     /gene="hcp"
                     /locus_tag="ACHURS_003364"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFR1239016.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKACEYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(10013..10912)
                     /gene="lysO"
                     /locus_tag="ACHURS_003365"
     CDS             complement(10013..10912)
                     /gene="lysO"
                     /locus_tag="ACHURS_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFR1239017.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         2713 a         2929 c         2943 g         2559 t
ORIGIN      
        1 gcaaatgtct ttttcatgac tgtagtatgc atggtgtcaa gataaaacca tggttgccag
       61 taaaatggac aaaagagtta ataaatgatt atttgtatgg ctgtttacta tctctgtact
      121 ctatctgtgc aagggatata tataatatga gcgccgggaa taacgtgaag gttgctgcgg
      181 atgctttcct tgaaattatt ttctctctta agaataaata ttgcattaaa ctattaagcg
      241 ctcaggaacg ggcgtttatt tacgaatttg caagaatgat cttcgcttat attaatgaca
      301 aaagcattga gatcttatta ctttcttgtt ttgcggcgtc tgaccaaaaa gcaatccaga
      361 ggtatatacc tcagtcacaa gatggggaag actttaggag tcatttacaa tataagttgc
      421 ctctttccgc tcattaatgt tgttggtgca acataataag acaggtaagc ggtaccctat
      481 aatgaaacca atatcgggtt atgcaacgga taatcgtaaa gacttaagtg ggtgtttttg
      541 acacaatgag atgatttcct aaactgatca aaaccttctt cccaacatgg catatcagtt
      601 cgataatgct ttagagatcg aaggcttaac aaacaaggag atcgatcatg caattttcta
      661 caaccccaac tctggaaggc cagaccatcg ttgaatattg cggtgtggtg accggcgaag
      721 cgattttagg tgccaatatt ttccgtgatt tctttgccgg tatccgcgat atcgttggcg
      781 gacgttccgg tgcgtatgaa aaagaactgc gtaaagcacg ggagatcgcc tttgaggaat
      841 taggctccca ggcgcgggcg ctgggggccg atgccgtcgt cggtattgat atcgactacg
      901 aaacggtcgg gcaaaacggc agtatgctga tggttagcgt cagcggtacg gcggtgaaaa
      961 cgcgtcgatg agaagagtct tctggctggt cgctgccgct ctgttattgg cagggtgtgc
     1021 aggcgaaaaa ggcattgtcg agaaagaggg atatcagctt gatacccgac gccaggcgca
     1081 ggcggcgtat ccgcgcatta aagtgctggt gatccactac accgcagatg attttgatag
     1141 ctcgctggcg acactgaccg ataagcaggt cagctcgcat tatctggtcc ctgcggtacc
     1201 accgcgatac aacggtaaac cgcgcatctg gcaactggtg ccggaacaag aactggcctg
     1261 gcatgcgggg attagcgcct ggcgcggggc aacgcgcctt aacgacacct ctattggcat
     1321 tgagctggaa aaccgtggct ggcaaaaatc ggccggagtg aaatattttg ccccgtttga
     1381 accgacacag attcaggcgc ttattccact ggcgaaagat attattgccc gttatcacat
     1441 caagccggaa aacgtagtgg cacatgcgga tatcgcaccg cagcgcaaag acgatccggg
     1501 gccattattt ccctggcagc aactggcgca gcaggggatt ggtgcctggc cggatgcgca
     1561 gcgggttaac ttttaccttg ccgggcgcgc gccgcacaca ccggtagata ccgcgtcatt
     1621 gctggagctt ttggcgcgct acggttatga cgttaaacct gatatgacac cgcgcgagca
     1681 gcggcgcgtg attatggcat tccagatgca tttccgcccg acgttatata acggcgaagc
     1741 ggatgcagaa actcaggcga ttgccgaagc attgctggag aaatacgggc aggattagcg
     1801 cggcagtttt ccgtggtcgc gtagccaggc ggcagttttc tcgatacctt catctagggt
     1861 gatgatcggc tgataaccta actcttcctg cgcacgcgta atatccagcg taaagtcaaa
     1921 attcaacttg gagacgccgt agtgggtcag cggcggctct tttgctgact tgcggcctaa
     1981 acgctccatg ctgcgggcga tcatatccag catcgggtag gggacggaac gaatacgaca
     2041 gtcaatattc aactcgtcga tcagcttctg cacgatgctg cgcagtgtgc gatgctcgcc
     2101 gttggtgata ttgtacacac gcccggaagg tagcttatcg caggcttcct ggcttgccag
     2161 ccacattgcg tgcacggtat tttcatagta ggtcatatcg accagcgcac tgccgccatg
     2221 cggtaacaga atactgccgt agtggtgcat catatgcgcc agacggggaa taaagacttt
     2281 atcgtgcggt ccgaacagac tttgtgggcg cagaatagta aagcgcgttt gtggattcgc
     2341 ctgcgaaagc atattgatca cttcttcgct ggctgctttg ctgcgggcaa actcgttggc
     2401 gaagcggtga gggcgaaaat cttctttaat atcgcgatgg tggtgataat cgaagtacag
     2461 ggagggggaa gagatatgaa taaagttacg cacaccccag gcgacagccc attcacccag
     2521 gcggcgagtg gcgcgaacgt tagccagatc gaaagcctgt tgtgtccccc agggtgaggt
     2581 aaagctggag cagtgccaca gcgtatcaat gcccgcgagc atcactttag cttgtgatga
     2641 aaccagctcg gtcagatccg ccggaacaaa ctctgcgccc attttttcca gcaatttgcc
     2701 cattgcctcg ttgcgaccgg tcgctcgcac gctgatgcct ttctggcata aaaactctac
     2761 cgcgtttcga cctaagccgc tggtggcgcc ggtaaccagt accttcatat caatccactg
     2821 ttgttgagaa aatgacgtgc gcattcttcc gtgatttccc ccatgatgca atgggaaaca
     2881 tgaaagaata acgcaggttt tgtcgattaa tctgtgcttt gttctgccag tctggcgatt
     2941 tgttttgcca ttccgcgaaa aataaacaga tgcgcgggga tcatcaataa ccagtaaaac
     3001 agccccggca taccgtgcgg atgccagaaa gcgcggacat cgatagtacg atagtcgcct
     3061 ttatcttcca ggctaaaaca cagtcgtccc agccccgacg ctttcatgcc aaataacaac
     3121 gtaagttgtt tttccggttc aacgacaatc actttccagc tatccaccgc atcgccagtc
     3181 tgtaaatatt cgcgctccgg gcggcctttc gccagcttat gaccgatcgc gcggtccatc
     3241 aacgcccgtg tctgccacaa aatattgcca aagaaataac gctctttacc gccgatttgg
     3301 ttcactacct gccataaagc agcaaggctg gcggacgttt taacggtaaa ccccgcctgt
     3361 ttggcaaaat aaccgtactc cggtcgccag cgggcaaagg cctgagcgtc gtagccccag
     3421 tcgctggagt tgaccagttt ttcctcctct ttcaacgtgc tacgtaccgc gtcatcgaaa
     3481 gcgatcagcc gttgtgggat gagtgcacgt agcgcggtat catccgccag cagatcgtgt
     3541 ttcagcccct gaatcaacgc cctggcggtg gtgggcggta cggaagtaat cacattgaga
     3601 aaccacaccg aaatccagcg ggtggggagg gggatgggga tcaaccagcg gcgcttaccg
     3661 ctcaccgcca taaaatgttc aaactgttgc tgataactga gcacctctgg tccggcggct
     3721 tcgaagatgc ggtgttcgct ggccggatga tctaacaacg ccaccagata gtgcagcaag
     3781 ttttccagcg cgatgggcgt ggtgcgtgaa cgtacccagc gtggcggcgt taacactggc
     3841 aggttgtaga ccatatcgcg catgacttcg aacgccgctg aacctgcgcc aacgataatt
     3901 ccggcccgaa gttcggtcac aggtacattc gcttcacgaa gaatgtccgc cgtagcctga
     3961 cgagcacgca gatgatccga ctgctcatgt ggcggggcct gcaacgaact gagaaagatt
     4021 aattgcttaa ctggtacttc acgtagcgca tcgcggacgt tgagagccac ctggcgctcc
     4081 tgagcgataa aatcgccgcc ttcgcccatg ctgtgcacca gaaaatagac ggtatcgata
     4141 tcctgcaaca gggccggaag gttatccggc cagctgagat cgactttatg gcaactgacg
     4201 tttgccagtt gcagctttgc aagcctgtcg acatgacgtg ccgccgccag gatctgatgc
     4261 ccttgctggc tgagtgtgcg caccagatgc tgaccaatgt agccactggc accgagaact
     4321 aaaatgcgtt gcggcacgtc tctctcctta acgcgccagg aatgcgcgcc agtgggcggc
     4381 gacttccgcc agttgttcgc gcgagacgtc aagatgcgtc accaggcgga caatcggcga
     4441 ggcgttaatc agcacgtttc tcgctttcat gtattcgcct aacgcggcag cattttcttc
     4501 cccgacgcga acaaacagca tattagtgtc ctgacgcatc acatccgcgc ctgcttcacg
     4561 cagttgctcc gccatccagg cggcgttgtc gtgatcttcc tgcaaccgtg cgacgttatt
     4621 tttcagggca tacatcccgg cggcagccag aatcccggac tgacgcatcc cgccaccggc
     4681 cattttccgc cagcggatgg cacgtttaat gtaatcacga ttaccgacga gtaatgaacc
     4741 gactggcgtc ccaagacctt tcgacaggca aatggtgaac gaatcacaat attgcgtgat
     4801 ctctttcagt tcgcagccgt aagccaccac ggcattaaag atgcgcgcac cgtcaacatg
     4861 cagcgccaga ttgcgcttgc gggtaaattc ccatgcttct ttcaggtatt cacgcggcag
     4921 cactttgccg ttgtgggtgt tttctagact gagtaatttg gtgcgggcga aatggatatc
     4981 gtcgggtttg attttcatcg ccactttatc cagcggtagc gtgccgtcgg cagccgcgtc
     5041 gatgggttgc ggttgaatac tgcccagcac cgctgcgcca ccggcttcaa acagataatt
     5101 atgcgcggcc tgaccgacaa tatactcttc gccgcgttcg cagtgactga gcagagcgac
     5161 caggttagcc tgggtgccgg tcggcagaaa aatggcggct tctttaccgg aaagctctgc
     5221 ggcgtagtcc tgcagagcat taacggtagg gtcgtctccg taaacgtcgt ccccaaccgg
     5281 ggcggccatc attgcttcga gcatggcgcg gctcggtcgg gtaacggtat cactgcgtaa
     5341 atcaatcatg gcatgtcctt attatgacgg gaaatgccac cctttttacc ttagccagtt
     5401 cgttttcgcc agttcgatca cttcatcgcc gcgtccgctg atgattgcgc gcaacatata
     5461 caagctaaat cctttggcct gttcgagttt gatctgcggt ggaatggcta actcttcttt
     5521 ggcgaccacc acatccacca gcaccggacc gtcgatggag aaggcgcgtt gcagggcttc
     5581 atcaacttca gacgcttttt ctacacggat acccgtaatg ccgcacgctt cggcaatgcg
     5641 ggcaaagttt gtgtcgtgta gttcggtgcc gtcagtcaaa tagccaccag ctttcatctc
     5701 catcgccaca aagcccagca cgctgttgtt aaagacgaca attttcactg gcagtttcat
     5761 ctgcactact gagaggaaat cgcccatcaa catgctaaaa ccgccatcgc cgcacatggc
     5821 gaccacctga cgttctggct ctgtcgcctg cgcacccagc gcctgcggca tggcgttagc
     5881 catcgaaccg tggttaaacg aacctaacag gcgacgcttg ccgttcattt ttagataacg
     5941 tgccgcccac accgttggcg taccaacgtc acaggtgaaa atagcgtcat cggcggcaaa
     6001 atgactaatt tgctgcgcca gatattgcgg gtgaatggct ttctcgctcg gtttagctaa
     6061 atcgtccagc cctttgcggg cgtcgcggta atcttccagc gctttatcca gaaacttgcg
     6121 atcggctttt tcttccacca atggaagcaa tgcacgcaga gtcgacttga tatcgccgac
     6181 cagtgccata tccaccttgc tgtgagcgcc gatgctggct gggttgatat caatctgaat
     6241 gattttggca tcggtcgggt agaaggcgcg gtagggaaat tgcgtgccga gtagcactaa
     6301 cgtgtcggcg ttcatcatgg tatggaaacc tgacgagaag ccgattaacc cggtcattcc
     6361 aacatcatac ggattatcgt attcgacatg ttctttaccg cgcagggcat gaacaatagg
     6421 cgctttaatt ttcccggcaa actcaactaa ctctttatgc gcccccgcgc agccgctgcc
     6481 acacatcagg gcgatattgc tggaataacg cagcagttgc gccagtttgc gtaactcttc
     6541 ttcttccggc gtcacgactg gttgtggcgc atgataccag tgcgtggttg ccccttctgg
     6601 cgcaggtttt aacgccacgt cgcctggtaa cacgacaacc gaaacgccac ggttaagcac
     6661 cgctttgcgc atggcaatcg ccagtacttg tgggatctgc tccgggctgg aaaccagctc
     6721 gcaatagtga ctacattcgc ggaatagctc ttgtgggtgg gtttcctgga aatagccgct
     6781 gccaatttcg ctggagggaa tatgagcggc aatcgccagt accggaacgt gattgcggtg
     6841 gcaatcgaac aggccgttga ttaagtgcag gttgccgggg ccgcacgatc cggcgcagac
     6901 cgccagttct ccgctaagtt gtgcttcagc gccagcggca aaggccgcca cttcttcgtg
     6961 gcgggtggac atccactcga tggtgcccat gcgattaaga ctgtcactaa gaccgttcag
     7021 agagtcgcct gtgactcccc agatgcgttt cacccctgcc gattcgagtg ttttggcgat
     7081 ataagctgca accgtttgtt tcatggttct ccatctcctg aatgtgataa cggtaacaag
     7141 tttagttcat ctgacggagg gggaagggat gggagagaaa ggaggcacta acggttaaat
     7201 agcccgatga aaggaatatc atcgggcata aggcgattat gcgagaacca aatccccctg
     7261 cggatggcag gagcaggcca gtacgtaacc ttcagcgatt tcggcgtcgg tcagcgtcat
     7321 tgtactgctc accgtatatt caccggaaac cacttttgtc ttacagcagc cgcaaacacc
     7381 cgcacggcag gcagcgacaa ccggaacgtt attgctttcc agcgcctcca gtagcgtggt
     7441 gccaaccggg gcgtaaaatt ctcgtgccgg ttgcagtttg gtgaatttca gaccgctggt
     7501 cgccgcttct gctactgggg tgaagaattt ctctttaaag aaacgcgtca cgccgagcgc
     7561 tttcacttcc tgctctaccc aatccatata cggagccggg ccgcaggtca tcacggtacg
     7621 tgaagctaag tcaggtacac ctgccagcag ttcgcgagtg agacgaccag cgataaagcc
     7681 ttcggtaacg ttattttctg ccaccagcgt taccggatag ttacgccact catcggcgaa
     7741 aataacatcc tgcggcgtac gcacgttgta gatcacccgc acatcggcct gtggacggtt
     7801 cttcgcaagc cagcgacgca tcgacataat cggcgtgacg ccgcagcctg ccgccagcaa
     7861 caggaattta tcttctgctt tatcgtcgca ggtaaattcc cccatcgcgt ccgaaagcca
     7921 gagataatca ccgcgtttta catcgcgcgt cagccactgg gagccgacac cgtcatcaat
     7981 ccgccgcacg gtcagggtga tatattcact cacgcctggc gtggaggaaa tggtgtaagc
     8041 acgcagcgtt tccgctgagt ttcgcacgct gaccagtgca tattgcccgg cgcgatatgg
     8101 gtagtaatcg tggcaaatca gggaaatcgt ccacacatcc ggcgtttctt gcgtaatgtg
     8161 atgaacctgc atccgccacg ggcattgatt cgttggcatc gtcatcgaca aactccttac
     8221 gcgctcaaca gttgcttcat gtcttcttca acagtggtga tagaacgcag gccgaatttc
     8281 tcgttcagca ccgccagcag gtctggtgtc aggaaaccag gtgcagtcgg gccggtgacg
     8341 atatttttca cgcccagaga aagcagcgtc agcagaatga cgatcgcttt ctgttcaaac
     8401 caggagagca ccagcgacag cggcagatcg ttcacaccac agcccagttt ctctgccaga
     8461 gtgacagcca gaataatcgc tgagtaagca tcgttacatt gacctgcatc taccagacgc
     8521 ggcagacctt cgatatcgcc aaactcaagt ttgttaaagc gatatttacc acaggcgagg
     8581 gtcaggatca ggcagtcatc cggcacgctg gtggcgaaat cggtgaagta gtggcgctcg
     8641 ccgcgtgcgc cgtcacagcc accaagcagg aagatatgac gcagtttttc acggctcacc
     8701 agatcaatca gcgtatcagc agcgccaagc agcgtctggc gaccaaaacc cacggtgata
     8761 aggtgcggaa tttcgctgta cgggaagcct gccatctgtt gcgcctgggt gataaccgca
     8821 gagaaatcat caccgtccag atgacgcacg ccaggccagc caacaatgct gcgggtccag
     8881 atacgatcgt cataagcgcc tacggttggg tcgatgatgc agttcgaggt catcacgatg
     8941 gggccaggga aacgagcgaa ctccacttgc tgattctgcc agccgctgcc gtagttaccg
     9001 accagatgct tgaatttacg cagctccggg tagccatgcg caggcagcat ttcgccgtgg
     9061 gtgtagacat taacgcccgt gccttcggtc tgttccagca ggttgtagag atctttgaga
     9121 tcgtgaccgg aaatcagaat gcatttaccc gccgtcgctt tgacgttgac ctgggttggc
     9181 gtcgggtgac cgtatttacc ggtttcgcct gcatccagaa tgctcatcac tttgaagttc
     9241 atctggccga tttccattga acactcaaga agcgcgttca tatcggcagg ccaggtcccc
     9301 agccacgcca tgattttatg gtactgggca taaatatcgt tgtcgtattg accgagaaca
     9361 tgcgcgtgtt ccatataggc cgccgcacct ttcaggccat acaggcacag cagacgcagg
     9421 ccgagaatgt tttcgccaat cgccgcttta tctttgttag gggtaaattc tgctgcctga
     9481 cgttgcagct cgccgagatc atcgctcacc agttgcaggt cagccatcgg gttatcgacg
     9541 cgcgcgttgg catctacagc caggcattgt gctttcagcg cctcgcgcaa ggcaatcgct
     9601 tcacgagcgt agccgacaat acgcggagaa tcgaagttaa cgttggtcag ggttgagaaa
     9661 aaggcacgtg gcgcgaagct gtcaacatcg tggttgatga tgccgtattc acacgctttt
     9721 accgcccagg cagaaagccc ttgcagcgcc gcgatgagta aatcctgaag gtcagaagtt
     9781 tccgccgttt taccacacat cccctgcgcg tatgagcagc cgtttcctgc cggagtacgg
     9841 atagtttgtt cacattgcac acaaaacatg atcacacctt ttaaagttat atttaatata
     9901 catgtttaag gttaagacgc ttaacgcggg gataaaaggg atttttcatg caactttaag
     9961 ggagattgat ttagcgcaat tttggcggca gggatctacc gccagagagg tattacgcag
    10021 agaaaaaggc gatgaggatc ggcactaaca ggctaagaat aaaaccgtga acaattgccg
    10081 ccgggaccat atccagcccg ccagtacgtt gaagaacggg cagggtgaaa tccattgatg
    10141 tggcaccgca taagcccagt gcagtagagc ggctgcggcg aatcagccca gggatcaaca
    10201 taatagcaat cagttcacgg gccagatcat taaaaaacgc cgcgctcccg attaccggac
    10261 caaaagattc ggtcaataaa ataccggaaa gagaatacca gccgaaaccg gaggccattg
    10321 ccagcgcggt attgatgggg agatcaagaa taaaggcgtt aattaaacca ccaattaatg
    10381 aactgacaac caccaccacg gcgacaatca ttccccggcg attaaggaca atctgcttta
    10441 aggtcatgcc attattgcgc aactgaatac caacgaggaa aagtagcaaa attaacgtgt
    10501 attcactggc ttcggtcgcg tgttgtaaga aagccagtcc actcagacca atggcaaaac
    10561 caatcactac tacgccgcac agttttagcg actccagcgc catcgcaata cgcgacggga
    10621 gtttttcttg ctgatggtgg ttgcgccacg gcaggcctcg ctccagccac atcagggcgg
    10681 caatattaca cagtaaaata acggtaatac tgacggcaga ataatgcaga atcgccaaca
    10741 ggttactggc gaggttatcg agaaacgcca gactgatacc cataaaaaag agaataaggt
    10801 aaaccatcca gcttaatagc tgattaataa cttttaacgc agcttgttgg cgaagcggaa
    10861 tgaggtaacc tacaatcagg ggaaccagaa tgattaacag cccagaaaac atgaaaaccc
    10921 agtccttgca aagatgaagt cgaaatgcgc gatgacacac tactgaaagc ggaaggacga
    10981 gtaaagttgc aattaaaagg aaatgttatg cataaggagc agtagagtat tcgttttcat
    11041 ttaaagatat tcttgcgctt taattacaaa ctgcaccgat gttggtggcg tcaaaatcgc
    11101 cgaggcgttc cctgaaggcc ggggcagccc acatggatgt gggc
//
LOCUS       DAZPDU010000145        10938 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZPDU010000145 DAZPDU010000000
VERSION     DAZPDU010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10938
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.147"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            69..1055
                     /gene="dcyD"
                     /locus_tag="ACHURS_003366"
     CDS             69..1055
                     /gene="dcyD"
                     /locus_tag="ACHURS_003366"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFR1239018.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGVIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCEALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEIILWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIGCISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            1070..1738
                     /gene="tcyL"
                     /locus_tag="ACHURS_003367"
     CDS             1070..1738
                     /gene="tcyL"
                     /locus_tag="ACHURS_003367"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFR1239019.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            1735..2487
                     /gene="tcyN"
                     /locus_tag="ACHURS_003368"
     CDS             1735..2487
                     /gene="tcyN"
                     /locus_tag="ACHURS_003368"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFR1239020.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            2717..3439
                     /gene="sdiA"
                     /locus_tag="ACHURS_003369"
     CDS             2717..3439
                     /gene="sdiA"
                     /locus_tag="ACHURS_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFR1239021.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAAHAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(3506..3730)
                     /gene="yecF"
                     /locus_tag="ACHURS_003370"
     CDS             complement(3506..3730)
                     /gene="yecF"
                     /locus_tag="ACHURS_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFR1239022.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(3717..3893)
                     /gene="yecU"
                     /locus_tag="ACHURS_003371"
     CDS             complement(3717..3893)
                     /gene="yecU"
                     /locus_tag="ACHURS_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFR1239023.1"
                     /translation="MIKIFIGHYINAFNSIVDVTLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            4189..4845
                     /gene="uvrY"
                     /locus_tag="ACHURS_003372"
     CDS             4189..4845
                     /gene="uvrY"
                     /locus_tag="ACHURS_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFR1239024.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            4842..6674
                     /gene="uvrC"
                     /locus_tag="ACHURS_003373"
     CDS             4842..6674
                     /gene="uvrC"
                     /locus_tag="ACHURS_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFR1239025.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            6731..7279
                     /gene="pgsA"
                     /locus_tag="ACHURS_003374"
     CDS             6731..7279
                     /gene="pgsA"
                     /locus_tag="ACHURS_003374"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFR1239026.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            7431..7506
                     /locus_tag="ACHURS_003375"
     tRNA            7431..7506
                     /locus_tag="ACHURS_003375"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7464..7466,aa:Gly,seq:gcc)
     gene            7561..7634
                     /locus_tag="ACHURS_003376"
     tRNA            7561..7634
                     /locus_tag="ACHURS_003376"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7593..7595,aa:Cys,seq:gca)
     gene            7647..7733
                     /locus_tag="ACHURS_003377"
     tRNA            7647..7733
                     /locus_tag="ACHURS_003377"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7681..7683,aa:Leu,seq:taa)
     gene            7928..8593
                     /gene="yecA"
                     /locus_tag="ACHURS_003378"
     CDS             7928..8593
                     /gene="yecA"
                     /locus_tag="ACHURS_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFR1239027.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTNHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(8655..9866)
                     /gene="tyrP"
                     /locus_tag="ACHURS_003379"
     CDS             complement(8655..9866)
                     /gene="tyrP"
                     /locus_tag="ACHURS_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFR1239028.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGV
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            10057..10296
                     /locus_tag="ACHURS_003380"
     CDS             10057..10296
                     /locus_tag="ACHURS_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFR1239029.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            complement(10334..10831)
                     /gene="ftnA"
                     /locus_tag="ACHURS_003381"
     CDS             complement(10334..10831)
                     /gene="ftnA"
                     /locus_tag="ACHURS_003381"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFR1239030.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
BASE COUNT         2809 a         2578 c         2839 g         2712 t
ORIGIN      
        1 ccttttttat ttacgcgttt tcagcgtgca taataagaaa atcacaacaa catacatcgg
       61 aggctgttat gccactgcat aatttaaccc gttttccacg gttggagttt atcggcgcgc
      121 caacgccgct cgaatatctg ccgcgctttt ctgattatct aggacgggaa attttcatca
      181 aacgggatga cgtcaccccc atggcaatgg gcggcaataa attacgtaag ctagaatttc
      241 tcgcggcaga tgctctgcgc gaaggtgccg atacgctgat tactgccggg gtgatccagt
      301 ctaaccatgt gcggcagact gccgcagttg cggcgaaact cggtctgcac tgtgtggcgc
      361 tgctggaaaa tcctattggc acaaccgcag aaaactattt aaccaacggc aatcgcttgt
      421 tgctggatct gttcaatacc cagattgaaa tgtgcgaagc actgaccgat cccaatgccc
      481 aactggaaga gctggcgacg cgagtcgaag cacaaggctt tcgcccgtat gtcattccgg
      541 ttggcggttc taatgctctt ggcgcgctag gttatgtgga gagtgcgctg gaaatcgcgc
      601 aacagtgtga aggggcggtt aatatttcgt cggtggtagt cgcatcgggc agtgccggaa
      661 ctcacgccgg actggctgtt gggctggaac acctgatgcc tgaaagcgaa ctgattggcg
      721 tgaccgtgtc gcgttccgtt gccgatcagt tgccgaaagt ggttaaccta caacaggcga
      781 ttgcgaaaga actggagctg accgcatcag cggaaattat actctgggat gactattttg
      841 cacctggcta cggcgtgccg aacgacgaag gcatggaagc agtgaaattg ctggcgcggc
      901 tggaaggcat tctgcttgat cctgtgtata ccggaaaagc gatggcgggg ctgattggct
      961 gtatcagtca gaaacgcttc aaagatgaag ggccgattct gtttattcat accggcggtg
     1021 cgcctgcgct gttcgcctat catccccacg tttagctccg ggaacgctca tgcaagaaag
     1081 tatacaactg gttattgatt ccttgccgtt cctgctgaaa ggggctgggt atacgctgca
     1141 actcagtatc ggcggcatgt tttttggctt actgctcggg tttattctcg cgctgatgcg
     1201 cctgtcgcca atctggcccg tgcgctggct ggcgcgtttt tatatctcta tttttcgtgg
     1261 tacaccgctc atcgcacaac tgtttatgat ctattacggc ctgccgcagt ttggtatcga
     1321 attagatccc attccgtcag caatgattgg tctgtcgctg aacactgccg cctatgccgc
     1381 cgaaacactg cgcgccgcca tttcttctat tgataaaggt cagtgggaag cagctgccag
     1441 tattggtatg acgccatggc agactatgcg tcgcgcgatt ttgccacagg cggcgcgggt
     1501 ggcgttgccg ccgctgtcga acagctttat cagcctggta aaggatacgt cgctggccgc
     1561 gacaatccag gtgccggagc tgttccgtca ggcgcagttg attacctcgc gcacgctgga
     1621 ggtgttcacc atgtacctgg cggcttcgct catctactgg atcatggcga cagtattatc
     1681 gacgttgcag aaccattttg agaatcaact taatcgccag gagagagaac caaaatgagt
     1741 gccattgaag ttaagaacct ggtgaaaaaa tttcacggtc agacggtgct gcacggtatc
     1801 gacctcgagg taaagcctgg cgaagtggtg gcaattatcg gtccgagtgg ttccggcaaa
     1861 accacgttgc tacgcagcat aaatctgctg gaacaaccgg aagcgggaac gatcaccgtt
     1921 ggcgatatca ctattgatac tgcacgttca ttaagtcagc aaaaatcgct gatccgccag
     1981 ttgcgtcagc acgtcgggtt tgtcttccag aactttaatt tgtttccgca tcgtacggtg
     2041 ctggaaaaca ttattgaagg gccggtgatc gtcaaaggcg aaccgaaaga agaggccacg
     2101 gcgcgcgctc gcgagctgct ggcgaaagtt gggctggccg gtaaagaaac cagctatcca
     2161 cgtcgtttgt ctggcggtca acagcagcgt gtagcgattg cgcgtgcgct ggcaatgcgt
     2221 cctgaggtga ttttgtttga cgagccaacg tcagcgctgg atccagagct ggtgggcgaa
     2281 gtcctgaaca caatccgcca gctggcgcag gaaaagcgca cgatggtgat tgtgacgcac
     2341 gaaatgagct ttgcccggga tgttgcggac cgggcgattt ttatggacca ggggcggata
     2401 gtcgagcagg gggcagcaaa agcgttattt gctgatcccc agcagcctcg cacccgccag
     2461 ttcctcgaga agtttctgct gcaataataa gaaaaaatca gccccgacga ttcacctgtc
     2521 ggggcgggac gccatttcaa gcgtgataaa actgcttaac aaatcagcat aactcattaa
     2581 taacataaga gaatgcgatg gcttgcaaaa gtaattcatt gcctgaataa tataaattat
     2641 atataaatct tatttatgtg atagtttgaa ttatcattat aaatgatact cactctcagg
     2701 ggcgttgcgg tttactatgc aggatacgga ttttttcagc tggcgtcgca cgatgctgtt
     2761 gcgttttcag aggatggagg ccgcagaaga ggtctaccat gaaatagagc ttcaggctca
     2821 gcagctggag tacgattact attcgttatg tgttcgccac ccggtaccat tcacgcgacc
     2881 taaagtggct ttctacacca attaccctga gtcgtgggtt agttattatc aggcaaaaaa
     2941 ttttctcgca attgatcctg tgctgaaccc tgaaaacttt agtcagggcc atttaatgtg
     3001 gaatgatgac ttattcagcg aagcacagcc attatgggaa gccgcgcacg cacatggttt
     3061 acgccgcggt gtcactcagt atttaatgct gccaaatcgg gcgctgggct ttttgtcctt
     3121 ttcccgttgc agcacgcgcg aaatacccat tcttagtgat gaactgcaat taaaaatgca
     3181 gttactggtg cgcgaaagtc tgatggctct gatgcgttta aatgatgaaa tagtgatgac
     3241 gccagagatg aatttcagca agcgcgaaaa agaaattctg aaatggacgg cggaagggaa
     3301 gacatcagca gagatagcga tgattttgtc aatctctgag aatacggtca atttccatca
     3361 gaaaaacatg cagaaaaaaa tcaatgcacc aaataagacc caggtggcct gttacgcggc
     3421 cgctactggc ttaatttgat cgcttttctt tctgcgtgcc agatgcaaaa atcggctgaa
     3481 aggcacgctc tcagccggtt ttatattact gacggtaggc ttgtttaatt tgcttaaccg
     3541 ttttggaaaa tactgcagcc tgggtttcgt cttcgatcag cgcaagctgt ttttccatct
     3601 ttaacatcac gcggcccgcg tcagcttgtc ccatcgcctg tagcatcagc gtcagcatcg
     3661 ctttcaggca ggagacttca tttgccagtt cttgattatt ctcggcagta gaaaaatcag
     3721 gcgtactcat ttattttcct cgtcatgttg caatgaaaat ttgcggtgaa aaatgttaag
     3781 gcggcggagt ataccataag ctttgctaaa aatagcagtg gttgtttttt gagcgtgacg
     3841 tcaacaatgc tattaaatgc gttaatataa tgaccaataa atatttttat catgaatgtt
     3901 ttttgcccgt attgttcggg ttaattaatg ttacatattc agcgggctga ttttcatttt
     3961 tgctgaataa agtcaatttt tgtcacattt catcgccggg cttactgtga aacgatcccg
     4021 gaagccgttg gtgacgggcg tgaccataac tgtggacaat cgaatttaca aaaacgagag
     4081 aaaaatcgaa tacccaccat ttttaacgtt tcaaagttgc aataaaaacc gctaatatac
     4141 gaatgactaa ctatcagtag cgttatccct atttctggag atattccttt gatcaacgtt
     4201 ctacttgttg atgaccacga actggtgcgc gcagggatac gacgcattct ggaagatata
     4261 aagggtataa aagtcgtcgg tgaggcatcg tgcggtgaag acgccgttaa gtggtgccgg
     4321 acaaatgccg ttgacgtggt gctaatggac atgagtatgc cgggcattgg cggtcttgag
     4381 gcgacgcgta aaatcgcgcg ttccacagct gatgtcaaaa tcatcatgct taccgtccat
     4441 acagaaaacc ctttaccagc gaaagtcatg caggccggtg ctgcgggcta cctcagcaaa
     4501 ggcgcggctc cgcaggaagt cgtgagtgcg attcgttctg tctattcagg gcagcgttac
     4561 attgcttctg atatcgctca acaaatggcg ttaagccaga tcgaaccaga aaaaacagaa
     4621 agcccatttg ccagtttgtc tgaacgtgaa ttgcagatta tgctgatgat caccaagggc
     4681 cagaaggtca atgagatctc agaacagctc aatctcagtc cgaaaacggt gaacagctac
     4741 cgctatcgta tgttcagtaa actaaacatt catggcgatg ttgagctgac tcacctggca
     4801 attcgccatg gtctgtgtaa tgcggagaca ttatcaagtc agtgagtgat cagtttgacg
     4861 caaaagcgtt tttaaaaacc gtaaccagcc agccaggcgt ttatcgcatg tacgatgctg
     4921 gtggtacggt tatctatgtc ggcaaagcga aagacctgaa aaaacggctt tccagctatt
     4981 tccgcagcaa cctcgcttcg cgcaaaaccg aagcgctggt cgcccagatc cagcaaattg
     5041 atgtaacggt tacgcataca gaaaccgaag cgctgttgct ggaacacaac tacatcaaac
     5101 tctatcagcc gcgttacaac gttttgctac gcgatgataa atcttatcct tttatcttcc
     5161 tgagtggcga tacccacccg cgtctggcga tgcatcgtgg tgcgaagcat gccaaaggtg
     5221 aatatttcgg cccgttcccg aatggctatg ccgtacgtga aacactggcg ctactgcaaa
     5281 agattttccc cattcgccag tgcgaaaata gtgtttatcg caatcgctcg cgtccgtgtc
     5341 tgcaatatca gatagggcgc tgtctgggac cgtgcgttga aggactggtg agtgaagaag
     5401 aatacgctca gcaggttgag tatgtgcgcc tgtttttgtc tggcaaagat gatcaggtgc
     5461 ttacgcaact cattagtcgt atggaaactg ccagccagaa tctggagttt gaagaagctg
     5521 cacgtattcg cgaccaaatt caggcggtgc gacgcgtcac cgaaaaacaa ttcgtttcca
     5581 ataccggcga cgacctcgac gttattggtg tggcgttcga tgcgggcatg gcttgtgtcc
     5641 acgtattgtt cattcgtcag ggcaaagtgc tcggcagccg cagctatttc ccgaaagtgc
     5701 ctggcggtac ggaactgagc gaggtggtag aaaccttcgt aggccagttc tatttacaag
     5761 gcagccagat gcgcacctta ccgggtgaga tcctgctcga ttttaatctt agcgataaaa
     5821 cgctgctcgc cgattccctt tcagaactgg cgggacgcaa gattaatgtt caaaccaaac
     5881 ctcgcggcga tagggcgcgt tatctgaaac tcgcgcgcac caatgcggcg acggccttaa
     5941 ccagcaaact ttcgcagcaa tctaccgttc accagcgact gaccgcgctt gccagcgtgt
     6001 tgaaattgcc ggaagtgaag cggatggagt gctttgacat cagccatacc atgggcgaac
     6061 aaaccgtcgc ttcctgtgtg gtgtttgatg ctaacggccc gctgcgtgcg gagtatcggc
     6121 gctataacat tacaggcatc acgccgggcg atgattatgc ggcgatgaat caggtgctgc
     6181 gtcggcgtta tggtaaagcc attgacgaca gtaagatccc ggatgtgatc cttatcgacg
     6241 gcggcaaagg ccagcttgcg caggcgaaaa atgtcttcgc cgaactggat gtctcatggg
     6301 ataaaaatca tccgctgcta cttggcgttg ccaaaggagc agatcgtaag gctgggctgg
     6361 aaacgctgtt ctttgagccg gaaggtgagg gattcagttt gccgccagat tctcccgcgc
     6421 tgcatgttat ccagcatatt cgcgatgaat cacatgatca cgcgattggc gggcaccgta
     6481 aaaaacgggc gaaggtcaaa aataccagtt ccctggaaac cattgaaggc gtcgggccaa
     6541 aacgtcggca aatgttgttg aaatatatgg gcggtttgca aggtttacgt aacgccagcg
     6601 tcgaggaaat tgcaaaagtg ccgggtattt cgcaaggtct ggcagaaaag atcttctggt
     6661 cgttgaaaca ttgatgtctc tgtagcaaca taggggtaat cttactgaca acagatagtt
     6721 acccgtcatt atgcaattta atatccctac gttgcttaca ctgttccgtg tcatccttat
     6781 cccattcttt gtattggtct tttatctgcc tgtcacctgg tcgccgtttg ccgccgcgct
     6841 cattttctgc gtcgcggcgg tgactgactg gttcgatggt tttctggcac gccgctggaa
     6901 ccagagtacc cggtttggtg ctttccttga ccctgtagca gataaagttc tcgtggctat
     6961 cgccatggtg ctggtaaccg agcattacca cagctggtgg gtgaccttac cggcagcaac
     7021 gatgatcgcc cgtgaaatta ttatttctgc gctacgcgaa tggatggcgg agttgggtaa
     7081 acgcagtagc gtggctgtct cctggattgg gaaagtgaaa accactgccc agatggtggc
     7141 gttggcatgg ctgctgtggc gtccgaacat ttgggttgag tacgccggta ttgcactttt
     7201 ctttgtggct gcggtactga ctctgtggtc aatgttgcaa tatttgagcg ctgcgcgtgc
     7261 agatttgctt gatcagtgat cgtttcggcg taattttcag caaacgatca aaagtggtga
     7321 aaaatatcgt tgactcatcg cgccaggtaa gtagaatgca acgcatcgaa cggcggcact
     7381 gattgccaga cgataataaa atcaagtgat taactgattg cttgatgaat gcgggaatag
     7441 ctcagttggt agagcacgac cttgccaagg tcggggtcgc gagttcgagt ctcgtttccc
     7501 gctccagttt aaaagacatc ggcgtcaagc ggatgtctgg ctgaaaggcc tgaagaattt
     7561 ggcgcgttaa caaagcggtt atgtagcgga ttgcaaatcc gtctagtccg gttcgactcc
     7621 ggaacgcgcc tccactttct tcccgagccc ggatggtgga atcggtagac acaagggatt
     7681 taaaatccct cggcgttcgc gctgtgcggg ttcaagtccc gctccgggta ccatgggaaa
     7741 gataagaata aaatcaaagc aataagcagt gtcgtgaaac caccttcggg tggttttttg
     7801 tgcctgcaac ttgtcgttac accctcctta atttttaatc accggcaaag ccactcaaaa
     7861 agtatctctg atacggaccg gcatgtaaga taggtgctgg cgagttgaga tccacaagga
     7921 aaagcgtatg aaaacgggac cgttaaacga aagtgagttg gaatggctgg acgatattct
     7981 gaccaaatac aacactaacc acgccatcct tgatgtggcg gagctggacg gtttattgac
     8041 ggcggtgttg agttctccgc aagagattga accggaacag tggctggttg ccgtgtgggg
     8101 tggggctgac tatgtgccgc gctgggcgtc agagaaagag atgacgcgct ttatgaacct
     8161 ggcttttcaa catatggccg ataccgcaga gcgtctgaac gaattcccgg agcagtttga
     8221 gccgttattc ggcttgcgag aagttgatgg cagcgagctg acgattgttg aggagtggtg
     8281 ctttggctat atgcggggcg tggcactttc tgactggtca acgttgcccg attcgttaaa
     8341 accagcgctg gaggcgattg cgctgcacgg tactgaggaa aacttcgagc gggtagaaaa
     8401 gatgtcgcca gaagcgtttg aagagagcgt agatgccatt cgactggcgg cgcttgatct
     8461 acatgcgtac tggatggcgc atccgcagga aaaagctgtt cagcaaccga tcaaagcaga
     8521 agagaaaccg gggcgtaacg atccttgccc gtgcggtagt ggtaagaaat ttaagcagtg
     8581 ctgcctgcat taagaggcca aattgggtgt cagcgttaat tctggcaccc aataaggaat
     8641 ttgaggctat ctgatcaccc cacttctggt aacaaccctg ccgcaatcaa aaattgcacg
     8701 ccaatcacag caataccaca gagaaacacc accaccagcg ccggacgacc acctttgacc
     8761 cggtaacccg cctgaggatt gtgctttctg ctttgccagg tcaacagcga agggataatc
     8821 aatgccagta ccgccagcgc cacaccggcg taacccagcg ccatcacaaa tcctcgtgga
     8881 taaaacagtg caaacgccaa cggcggcaga aaggtaattg caccagtttg caaccgtcca
     8941 ccaacggtat ttgaacgctg aaataaatca gccagataat caaataagcc taacgcaacg
     9001 ccgagaaatg acgtggcgag ggctaaatca gcaaataaat gcactgccag ctcaacatgc
     9061 ggagaggcca ccatttcgcg taacgcctgt aacagcccgt ttaatccagc atgattagcc
     9121 agcaatccca taaaggttgt tgaatcaatg ctgccaagcg ttgccacctg ccagaaaata
     9181 tatgccacca gggggatcgc actaccggtt ataaacaccc agcgtagctt acgaacgttg
     9241 ccatccatat agctgacaat actcggcacg ctaccgtgaa aaccaaatga ggtaaaaatc
     9301 accgggattg ctgacagagc cagcccctgt tgcaacggca gggttaaaag attcactttg
     9361 tgaatatgcg gtagcagcaa taccagcatc accaccagaa aaataatttt ggcgctgaac
     9421 agaaaacggt taaataaatc gaccagcgat gttccaacac aaaccacgcc accggcaaca
     9481 aaagtgaaca acagcacgcc agcggttgcc gacattgaaa tacctgtcca gtcgctgatg
     9541 ctggaggcca acaattcacc gacaccgctg atgtatgccg cagtcagagc atacattaag
     9601 aacatcatac tgaagcccgt cagccattga ccgtagcgtc ccagatagcg ttttgccagc
     9661 gtgcccagac cggtatctgc cggaacatgc tggtacacct ccagcagtaa tagcgccgtg
     9721 tagcacatca acgcccaaag cccaatcaac aagattaacg taacgctaaa accaacaccg
     9781 gccgcagcca gcggcattgc cagcatgcct gcgccaattg tggttcccgc cacgataaaa
     9841 acacttccca gggttctgtt tttcacgctt tcttctgtcc tgacgatctt tatgagtgat
     9901 atctgcggcg caggttaagg cataacggct gcttcgtcaa accgacgtta catatagtgt
     9961 aaatataaat gtacaataaa aagcattcag tcctgcgctg gcgcaaagtc ttcataagcg
    10021 gcaatcgcta cgctaggctt tttttgagga ggaacgatgg actctattca cggtcatgaa
    10081 gtgttaaata tgatgattga atcaggcgag caatatacgc atgccagtct ggaagctgcg
    10141 attaaagcgc gttttggtga acaggcacgt tttcacacct gctcggcaga agggatgaca
    10201 gcgggagagc tggtagcgtt tctggcagca aaaggcaaat ttataccttc gaaagacggt
    10261 ttttcgaccg atcagagtaa gatttgccgt cactgatttg aaacggcggc agtaaacctg
    10321 ccgccggaga gcattagttt tgtgtgtcga gggtagagag ttctttgtcg ataaaataca
    10381 gaccttcgcc gcttttgcct gccaggctta atttatcaat aatcgatttg aacagtttct
    10441 cttcttcatg ctgctcagaa acataccact gcaggaaatt aaatgttggg tagtcctgat
    10501 tggtcattgc agcatgagcc agttcgttaa ttttctgggt gattaattgt tcgtgtttat
    10561 aggtttcctg gaataattca tcaagtgagg aatattcagc aaacggagat tcaacggtat
    10621 taatacgcgg taaattgccg gtatcagtca ggtaatcaaa cagacgctgc atatgcgtca
    10681 tctcttcctg ggcgtgacgg cgcaggaacg cggcagcacc ttcgaaggta tgatagctgc
    10741 accaggcgct catttgctga taaagcagtg aagagtacag ttccaggttc atctgctcat
    10801 taagtttttc aatcatttct ggtttcagca tgatagtgct ccacaaaggt tatatttgtt
    10861 gctgtggcgt cactataatt tgttatttta ttatttgcaa aaggtaaaat ataaacttca
    10921 attattaaaa atacgaat
//
LOCUS       DAZPDU010000146        10853 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZPDU010000146 DAZPDU010000000
VERSION     DAZPDU010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10853
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.148"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            80..421
                     /locus_tag="ACHURS_003382"
     CDS             80..421
                     /locus_tag="ACHURS_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFR1239031.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASKIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQRQQAEKAQEKEQRLSLNVSLLNP
                     VENTTHIDIYI"
     gene            complement(412..1338)
                     /locus_tag="ACHURS_003383"
     CDS             complement(412..1338)
                     /locus_tag="ACHURS_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1239032.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFCIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGWEGAPLYQI"
     gene            1428..2426
                     /locus_tag="ACHURS_003384"
     CDS             1428..2426
                     /locus_tag="ACHURS_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFR1239033.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(2423..2641)
                     /locus_tag="ACHURS_003385"
     CDS             complement(2423..2641)
                     /locus_tag="ACHURS_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFR1239034.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(2643..4658)
                     /gene="ligA"
                     /locus_tag="ACHURS_003386"
     CDS             complement(2643..4658)
                     /gene="ligA"
                     /locus_tag="ACHURS_003386"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFR1239035.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEVLEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(4729..5715)
                     /gene="zipA"
                     /locus_tag="ACHURS_003387"
     CDS             complement(4729..5715)
                     /gene="zipA"
                     /locus_tag="ACHURS_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFR1239036.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPIQQPPEAQVPPQHAPRPAQPMQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            5945..6706
                     /gene="cysZ"
                     /locus_tag="ACHURS_003388"
     CDS             5945..6706
                     /gene="cysZ"
                     /locus_tag="ACHURS_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFR1239037.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            6891..7862
                     /gene="cysK"
                     /locus_tag="ACHURS_003389"
     CDS             6891..7862
                     /gene="cysK"
                     /locus_tag="ACHURS_003389"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFR1239038.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            8246..8503
                     /gene="ptsH"
                     /locus_tag="ACHURS_003390"
     CDS             8246..8503
                     /gene="ptsH"
                     /locus_tag="ACHURS_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFR1239039.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            8548..10275
                     /gene="ptsI"
                     /locus_tag="ACHURS_003391"
     CDS             8548..10275
                     /gene="ptsI"
                     /locus_tag="ACHURS_003391"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFR1239040.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            10316..10825
                     /gene="crr"
                     /locus_tag="ACHURS_003392"
     CDS             10316..10825
                     /gene="crr"
                     /locus_tag="ACHURS_003392"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFR1239041.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
BASE COUNT         2641 a         2797 c         2691 g         2724 t
ORIGIN      
        1 gatttgtaag attgattttt cttctcaagt tccttgttct tctgccgata ttacctcgta
       61 cagtgacgag gactgaacaa tggccactat ctcttcaact tcaattcctt ccatacaaac
      121 acagagtagt aacagggcat cacaaggtag cgatgttgct tcaaaaatcg cgcggataag
      181 tcagcaaatc atcaaactca ctcaacaaat taaagagatt gtagacacta gcggcagtgc
      241 tgaagacaaa caaaaacagg ccgaattgat tcaacagcaa atcaccctac tggaaacaca
      301 actggcgcag ttgcagagac agcaggcaga aaaggcgcag gagaaagagc agcgtttgtc
      361 tctgaatgta tcccttctaa atcctgttga aaatacgact catatcgata tctatatctg
      421 atacaacgga gccccttccc aaccagccat tagcgcattg ccctggtctc gtaccacgtc
      481 ccataacgct tcagcggcgg tggaaagtga acggttcttc ctgcgcacca gcatcaactg
      541 gcgttcaaca accggcgtaa tgcgctttac caccagtggg ctaccttcag gcaatggtag
      601 cgccagtgcg ggaagaatac tgatgccgat gcctgcggct accatcggga acagcgtcgc
      661 cggatgtcca atctcctgca caatattcgc ctgaatacca ttgcgcgcca gcgctgcgtc
      721 aatcagcggt cggctgcctg acgcgtaatc ctgcaacacc aatttcgctc cctgtaatgc
      781 ctgccagggt acataatctt ccacggccaa agcgctatcg cgatggcaaa gcagaaagaa
      841 aggctcggaa agaatcgctt cacattgcag atcgcccact gggcctggat cgatgacgat
      901 gcaaaaatcg acatctccct ggcgaatact ttccatcacc cactgctgcg gacgatcgtg
      961 caggacaaac tgaatatctg gatagcggcg atggctttca gcaatgcatt gcggaataag
     1021 atgcgcggaa atggtctggc tggcagcgac ccgaactttt ccgctcagtt gttgccccat
     1081 acgcccggta tcgcgcaacg tgctgttaag ttcatccagc agtcgctcaa gacgcaaagc
     1141 caactgctgc cctgcatctg taagcaccac ttcacgcgtg gttctgtcca gcaggcgaac
     1201 accagtatga ttttccagct ccttcacact gtgacttact gccgactggc tcaagccgat
     1261 acgctctcct gcacgactaa aacttttctc ctgcgctact gtgacgaaaa cctttaattg
     1321 ttttaaagaa taattcatct gttttcttca tgaatggatg caataaatca attttatttc
     1381 tcaaactgga agaagcacaa tagaaccatc gatcatctgg agtctttatg aaactttttc
     1441 gtatcctcga tcctttcacc ttaaccctga tcacggtggt gttgctggcc tctttctttc
     1501 cggccagagg cgatttcgtc cccttctttg aaaatctgac caccgcagct attgccctgc
     1561 tgttctttat gcacggcgcg aagttgtcgc gtgaggcgat tattgctggc ggtggtcact
     1621 ggcgactgca tttgtgggta atgtgcagca ccttcgtgct gtttccgatt ctgggtgtac
     1681 tgtttgcctg gtggaaaccg gtaaatgtcg acccgatgct ctactccggt tttctctact
     1741 tgtgcattct cccggctacc gtgcagtctg caatcgcctt cacgtcaatg gcgggcggta
     1801 acgtcgcggc ggcggtttgt tctgcgtcag catccagcct gctggggatt ttcctttcac
     1861 cattgctagt tggtctggtg atgaatgttc acggtgcagg gggcagcctt gagcaggtcg
     1921 gtaaaattat gctgcaactg ctgctgccgt ttgtgttggg gcatctttcc cggccgtgga
     1981 ttggtgactg ggtgtcgcgc aataaaaaat ggattgcgaa aactgaccag acgtccattc
     2041 tgttggtggt ttatacagcg ttcagcgaag ccgtcgttaa tggtatctgg cataaagttg
     2101 gctggggatc attgctgttt atcgtggtgg tcagctgcgt tcttctggct atcgtgattg
     2161 tagttaacgt ctttatggca cgccgactgg gcttcaataa ggcagatgaa attactatcg
     2221 tcttttgtgg ttcgaaaaag agtctggcaa atggcatccc gatggcaaac attctgttcc
     2281 ccacatcggt gatcggtatg atggtgctgc ccctgatgat tttccatcag atccaattga
     2341 tggtctgtgc ggtgctggcg cgtcgataca aacgccagac cgaacagtta caggcgcagc
     2401 aggaaagcag cgccgataaa gcttaaagcg gacgcttcag gggctggacc agttgcgtca
     2461 gcccctcggt tttaatcagc agcgtgattt gcattagctc accgagcttc cctgccggga
     2521 actcatcttt gcgggcaaac cacagcagat actcctccgg caggtcgatt aagcgacgcc
     2581 ctttgtattt gccaaacggc attatcgtat tggctatttc aatcagctgc tctttttcca
     2641 tctcagctac ccagcaaacg cagcatttcc gcttcgtcga tgacttcaat gcccagttcc
     2701 tgcgccttcg ccagtttaga tcctgcagct tcacccgcta tcaccagatc ggttttcttc
     2761 gacacgctgc ccgcgacttt cgcgcccagt tcgaccagtc gagctttagc gtcatcacgc
     2821 gacatctggc ttaagctgcc cgtaagcacc acggttttac cagcaaacgg gctgtcaatc
     2881 tcttccgcgt tgataacgat cggcgcaggc cagtgaacac cttccgccaa cagctcgctg
     2941 atgacattgc ggttgctttc ttcggcaaag aagttgtgaa cgtgggatgc aacgacaatg
     3001 ccaacatcag gcaccttttg cagctcttca atcgaagcgg cttccagcac ttccagcgtg
     3061 ccgaaatatg ccgccagacc tgctgcggtg gcctcgccga cttcacggat gccaagtgca
     3121 tagaggaagc gagcaaaggt ggtttctttc gctttttcca gcgcgttaac cacgttttgt
     3181 gccgattttg gccccatacg ctccagtccg gtcagtttgc ctgcggtgag tttgaacaga
     3241 tctgccggag tgtggacata ttctttttca accagctgat cgatgatttt gtcgcccatt
     3301 ccgtcaacat ccatcgcacg gcgggaaaca aagtgtttca gcgactcttt acgctgcgca
     3361 ccgcaaatca ggccaccggt acagcgggca accgcttcac cttccacacg ctcaacgtca
     3421 gaaccacata ccggacaatg cgtcgggaat acaacctcac gggtatcttc cgggcgttca
     3481 gaaagcacga cgttaaccac ctgcgggatc acgtcgccag cgcggcgaat caccacttta
     3541 tcgccaatgc gtaaaccaag acgttcgatt tcatccgcat tgtgtaaggt tgcgttactc
     3601 accagcacgc cagcaacatg gacaggttcc agacgcgcaa caggcgtaat cgcgccagta
     3661 cgcccaacct gaaactcgac gtcacgcaca aaggtcatct gctcctgcgc cgggaattta
     3721 aacgctaccg cccagcgcgg ggcacgcgcg acaaagccaa gctgctcctg ctgttccagt
     3781 gagttgacct taatcaccac gccgtcgata tcaaagccca gcgtcgggcg gtcttcttcc
     3841 actttgtgat agaacgccag cacttcttcc gccgattcac aaagcgttac ccgatcgctg
     3901 accggcaacc cccacttttt aaattgcagt aaacggccaa gatgagtatc cggcagctcg
     3961 ccaccttcca gaacaccaac gccatagcag aaaaaagtga gcggtcgctt cgctgtaata
     4021 cgcggatcaa gctgacgcag tgaaccagct gccgcattac gtgggttagc aaaaactttc
     4081 ccgcccgtgc gtcgcgcatc ttcgttaatc ttttcgaacc ccgcctgcgg caggaacact
     4141 tcaccacgca cttccagacg cgccgggata ttctctccgt gcagcttcag cggaatggcg
     4201 cgaatagtac gcacattaga cgtgatatct tccccggtgg tgccatcgcc acgggtcgcg
     4261 gcactgacta aaacgccatt ttcatacaga atactgacgg caaggccatc cagcttcagc
     4321 tcacagcacc aggtgacttt ctcgttgttt ttcagacggt cctgcacacg tttgttgaaa
     4381 gcaagaaagc tttcttcatc aaaaacgtta tccagtgaca gcattggtac ttcatggcgt
     4441 atctggctga aagccgccag cggcgcagca cctacacgtt gggtaggcga atcaggcgta
     4501 atcagttctg gatgtttggt ttccagctcg cgcagttcgc gcatcagcct gtcgtattca
     4561 gcgtcgggaa tttccggcgc atccatcaca tgataaagat attcatgatg gcgaagcgtc
     4621 gttcgcagtt ctgtcagttg ttgttcgatt gattccatat cgcaccatca atgctaaaaa
     4681 cccccgacaa gcgggggttc gaagaggagt taatttgcct taagtgtatc aggcgttggc
     4741 gtctttgact tcgcggatga tgtcctggta ctcgcgcaat ttctgcggag tcatcatacg
     4801 gcgctgatcg tcaagcacga caccgcccac ttcatcggca atatgctgcg cagattgcag
     4861 catcagcttg aagttctgca gctcgtcacc gtaagacggt acctgcataa agatagtgac
     4921 acccggagta gtgaaatcct tcatttcagg atcaaaggtt cccggtttca ccatattcgc
     4981 caggctgaat aacgccgggc cgctgccatc cgggctaaga tgacgatggt aaatattcat
     5041 atcgccaaaa atgaagcccg cttgttgaat gctgttaaga agcagttcac cgtttagctc
     5101 gctaccgtga tgcgccgcga cgttcatgat aatcaccgct tctttgcgct tcggtttatc
     5161 cataactgga gccggttccg ctacaggctc aggctgtggt gccgctacgg gttctgcagg
     5221 ctggaaagcc tgttgtgccg gttgcggtgc tgaatgcaca ggctgcggcg ctggcgcgac
     5281 ctgtggcgaa actggctgct gcaacggctg ttcaggctgc ggctgatagg caggctgctg
     5341 catcggctgc gctggacgcg gagcatgttg cggcggtacc tgcgcttcag gcggctgctg
     5401 gatcggttga cgcggctgcg cagacgcata aggcggttgg tactggtgtt gcggcgacgg
     5461 acgagcagcc tcatgctcct gagcgttagc cggggcatga ttcacgcggt gaacacgaac
     5521 ctcaccaacg ccctcatcat cttcgacatc ctcgtcataa gaatcgtcgt cacgttttga
     5581 cttcattcgt tttaatggcc gatcgcggaa catagaagat cgttctttac ggctggtcca
     5641 gaaaccatgt accagtaaag cgattatggc gatcgcgcca acaatgatta atatcagacg
     5701 caaatcctgc atcattatat tctctgttgt tctaacacct tgccaccacg gcaaacattt
     5761 actcactaag agtatttgcc gattacctca agtgcaagtg cactattaac tttcacagca
     5821 caaagataga tgaaatcgtg ctttttgctg ttttttcgaa catatcctaa ctgtccattg
     5881 cgcaattacc cggtaaaata cgcagaattt tcctgggatt ggtcaaaagg agctcatcct
     5941 gattatggtt tcatcattca catctgcccc acgcagcggt ttttactatt ttgcgcaagg
     6001 ctggaagctc gtctcgcaac ctgggattcg gcgtttcgtt attttaccgc tgctggtcaa
     6061 tattttgttg atggggggcg cattctggtg gctctttaca cagctcgatg tctggatccc
     6121 gactctcatg agttacgttc cggactggct acaatggctg agttatctgt tgtggcctct
     6181 ggctgtcatc tctgtgctgt tagtgtttgg ctatttcttc tccacgattg ctaactggat
     6241 tgccgctccg tttaacggtt tattggctga acaactggaa gcacgattga ctggcgctac
     6301 accgccagat accgggattt tcggtatcat gaaagatgtg ccgcgaatca tgaaacgcga
     6361 atggcaaaaa tttgcctggt atctgccgcg cgcaattgta ttactaattc tttacttcat
     6421 ccctggtatt gggcaaaccg tcgcgccggt actgtggttc ctgtttagcg cctggatgtt
     6481 agccatccag tattgcgatt accccttcga taaccacaaa gtgccgttta aagagatgcg
     6541 caccgccctg cgcacacgca aaatcaccaa tatgcagttt ggtgctttaa ccagcctgtt
     6601 tacgatgatc ccgctgctta atctgttcat catgcccgtt gccgtttgtg gcgcgacggc
     6661 gatgtgggtc gattgctatc gcgataaaca cgcgatgtgg cggtaacaat ctaccggtta
     6721 ttttgtaaac cgtttgtgtg aaacaggggt ggcttatgcc gccccttatt ccatcttgca
     6781 tgtcattatt tcccttctgt atatagatat gctaaatcct tacttccgca tattctctga
     6841 gcgggtatgc tacctgttgt atcccaattt catacagtta aggacaggcc atgagtaaga
     6901 tttttgaaga taactcgctg actatcggtc acacgccgct ggttcgcctg aatcgcatcg
     6961 gtaacggacg cattctggcg aaggtggaat ctcgtaaccc cagcttcagc gttaagtgcc
     7021 gtatcggtgc caacatgatt tgggatgccg aaaagcgcgg cgtgctgaaa ccaggcgttg
     7081 aactggttga accgaccagc ggtaataccg ggattgcact ggcctatgtt gctgccgctc
     7141 gcggttacaa actcaccctg accatgccag aaaccatgag tattgaacgc cgcaagctgc
     7201 tgaaagcgtt aggtgcaaac ctagtgctga cggaaggtgc taaaggcatg aaaggcgcaa
     7261 tccaaaaagc agaagaaatt gtcgccagca atccagagaa atacctgctg ctgcaacaat
     7321 tcagcaatcc ggcaaaccct gaaattcacg aaaaaaccac cggtccggag atctgggaag
     7381 ataccgacgg tcaggttgat gtatttattg ctggcgttgg gactggcggt acgctgactg
     7441 gcgtcagccg ctacattaaa ggcaccaaag gcaagaccga tcttatctct gtcgccgttg
     7501 agccaaccga ttctccagtt atcgcccagg cgctggcagg tgaagagatt aaacctggcc
     7561 cgcataaaat tcagggtatt ggcgctggtt ttatcccggc taacctcgat ctcaagctgg
     7621 tcgataaagt cattggcatc accaatgaag aagccatttc taccgcccgt cgtctaatgg
     7681 aagaagaagg tattcttgca ggtatctctt ctggagcagc tgttgccgcg gcgttgaaac
     7741 tacaagaaga tgaaagcttt accaataaga atattgtggt tattctacca tcatcgggtg
     7801 agcgttattt aagcaccgca ttgtttgccg atctcttcac tgagaaagaa ttgcaacagt
     7861 aatgccagct tgttaaaaat gcgtaaaaaa gcaccttttt aggtgctttt ttgtggcctg
     7921 cttcaaactt tcgcccctcc tggcattgat tcagcctgtc ggaactggta tttaaccaga
     7981 ctaattattt tgatgcgcga aattaatcgt tacaggaaaa gccaaagctg aatcgatttt
     8041 atgatttggt tcaattcttc ctttaacggc ataatgttta atgacgtacg aaacgtcagc
     8101 ggtcaacacc cgccagcaat ggactgtatt gcgctcttcg tgcgtcgcgt ctgttaaaaa
     8161 ctggcgctaa caatacaggc taaagtcgaa ccgccaggct agactttagt tccacaacac
     8221 taaacctata agttggggaa atacaatgtt ccagcaagaa gttaccatta ccgctccgaa
     8281 cggtctgcac acccgccctg ctgcccagtt tgtaaaagaa gctaagggct tcacttctga
     8341 aattactgtg acttccaacg gcaaaagcgc cagcgcgaaa agcctgttta aactgcagac
     8401 tctgggcctg actcaaggta ccgttgtgac tatctccgca gaaggcgaag acgagcagaa
     8461 agcggttgaa catctggtta aactgatggc ggaactcgag taatttcccg ggttctttta
     8521 aaaatcagtc acaagtaagg tagggttatg atttcaggca ttttagcatc cccgggtatc
     8581 gctttcggta aagctctgct tctgaaagaa gacgaaattg tcattgaccg gaaaaaaatt
     8641 tctgccgacc aggttgatca ggaagttgaa cgttttctga gcggtcgtgc caaggcatca
     8701 gcccagctgg aaacgatcaa aacgaaagct ggtgaaacgt tcggtgaaga aaaagaagcc
     8761 atctttgaag ggcatattat gctgctcgaa gatgaggagc tggagcagga aatcatagcc
     8821 ctgattaaag ataaacacat gacagctgac gcagctgctc atgaagttat cgaaggtcag
     8881 gcttctgccc tggaagagct ggatgatgaa tacctgaaag aacgtgcggc tgacgtacgt
     8941 gatatcggta agcgcctgct gcgcaacatc ctgggcctga agattatcga cctgagcgcc
     9001 attcaggatg aagtcattct ggttgccgct gacctgacgc cgtccgaaac cgcacagctg
     9061 aacctgaaga aggtgctggg tttcatcacc gacgcgggtg gccgtacttc ccacacctct
     9121 atcatggcgc gttctctgga actgcctgct atcgtgggta ccggtagcgt cacctctcag
     9181 gtgaaaaatg acgactatct gattctggat gccgtaaata atcaggttta cgtcaatcca
     9241 accaacgaag ttattgataa aatgcgcgct gttcaggagc aagtggcttc tgaaaaagca
     9301 gagcttgcta aactgaaaga tctgccagct attacgctgg acggtcacca ggtagaagta
     9361 tgcgctaaca ttggtacggt tcgtgacgtt gaaggtgcag agcgtaacgg cgctgaaggc
     9421 gttggtctgt atcgtactga gttcctgttc atggaccgcg acgcgctgcc cactgaagaa
     9481 gaacagtttg ctgcttacaa agcagtggct gaagcgtgtg gctcgcaggc ggttatcgtt
     9541 cgtaccatgg acatcggcgg cgacaaagag ctgccataca tgaacttccc gaaagaagag
     9601 aacccgttcc tcggctggcg cgctatccgt atcgcgatgg atcgtaaaga gatcctgcgc
     9661 gatcagctcc gcgctatcct gcgtgcctcg gctttcggta aattgcgcat tatgttcccg
     9721 atgatcatct ctgttgaaga agtgcgtgca ctgcgcaaag agatcgaaat ctacaaacag
     9781 gaactgcgcg acgaaggtaa agcgtttgac gagtcaattg aaatcggcgt aatggtggaa
     9841 acaccggctg ccgcaacaat tgcacgtcat ttagccaaag aagttgattt ctttagtatc
     9901 ggcaccaatg atttaacgca gtacactctg gcagttgacc gtggtaatga tatgatttca
     9961 cacctttacc agccaatgtc accgtccgtg ctgaacttga tcaagcaagt tattgatgct
    10021 tctcatgctg aaggcaaatg gactggcatg tgtggtgagc ttgctggcga tgaacgtgct
    10081 acacttctgt tgctggggat gggtctggac gaattctcta tgagcgccat ttctatcccg
    10141 cgcattaaga agattatccg taacacgaac ttcgaagatg cgaaggtgtt agcagagcag
    10201 gctcttgctc aaccgacaac ggacgagtta atgacgctgg ttaacaagtt cattgaagaa
    10261 aaaacaatct gctaatccac gagatgcggc ccaatttact gcttaggaga agatcatggg
    10321 tttgttcgat aaactgaaat ctctggtttc cgacgacaag aaggataccg gaactattga
    10381 gatcattgct ccgctctctg gcgagatcgt caatatcgaa gacgtgccgg atgtcgtttt
    10441 tgcggaaaaa atcgttggtg atggtattgc tatcaaacca acgggtaaca aaatggtcgc
    10501 gcctgtagac ggtaccattg gtaaaatctt tgaaaccaat cacgcgttct ctatcgaatc
    10561 tgatagcggc gttgaactgt tcgtccactt cggtatcgac accgttgaac tgaaaggcga
    10621 aggcttcaag cgtattgctg aagaaggtca gcgcgtgaaa gttggcgata ctgtcattga
    10681 atttgatctg ccgctgctgg aagagaaagc caagtctacc ctgactccgg ttgttatctc
    10741 caacatggac gaaatcaaag aactgatcaa actgtccggt agcgtaaccg tgggtgaaac
    10801 cccggttatc cgcatcaaga agtaattctt gccgcagtga aaaatggcgc cca
//
LOCUS       DAZPDU010000147        10838 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZPDU010000147 DAZPDU010000000
VERSION     DAZPDU010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10838
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.149"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..138)
                     /locus_tag="ACHURS_003393"
     CDS             complement(<1..138)
                     /locus_tag="ACHURS_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR1239042.1"
                     /translation="MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLA
                     YE"
     gene            complement(385..858)
                     /locus_tag="ACHURS_003394"
     CDS             complement(385..858)
                     /locus_tag="ACHURS_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239043.1"
                     /translation="MKYDDVSELMIKREHNILIVGNSASHIDKFFKGYSIPGSKYYDF
                     TQINSDSDVERNEDAVSFIRDAMNSEEQTIIFNCAGWPDLGGGSAVSQFAMMVRKFGK
                     QLIVAVSEKDAIKLKDNFDIIGMLSYGKENFIAMSHTKNELTGGKRRYRIKNGCK"
     gene            complement(1013..1603)
                     /locus_tag="ACHURS_003395"
     CDS             complement(1013..1603)
                     /locus_tag="ACHURS_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFR1239044.1"
                     /translation="MKLIIFILIVLIIAALLIRIILKSVNQHSPLLMQLHAAGIRTGD
                     AERILSGGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVERRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            complement(1641..1850)
                     /gene="hha"
                     /locus_tag="ACHURS_003396"
     CDS             complement(1641..1850)
                     /gene="hha"
                     /locus_tag="ACHURS_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFR1239045.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLLNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            complement(1896..2357)
                     /locus_tag="ACHURS_003397"
     CDS             complement(1896..2357)
                     /locus_tag="ACHURS_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFR1239046.1"
                     /translation="MRKYIPLVLFIFSWPVLCADIHGRVVRVLDGDTIEVMDSRKAVR
                     IRLINIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNVN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            complement(2602..2814)
                     /locus_tag="ACHURS_003398"
     CDS             complement(2602..2814)
                     /locus_tag="ACHURS_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFR1239047.1"
                     /translation="MNGTGRDSQNSGVWRYRQVGRRAVRLEVRGDWPGAAEAWLRAAQ
                     SAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            complement(2943..3503)
                     /locus_tag="ACHURS_003399"
     CDS             complement(2943..3503)
                     /locus_tag="ACHURS_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFR1239048.1"
                     /translation="MTEQKRPVLTLKRKTEGETPTRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELTAKKAQARQALSIYLNLPSLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEV
                     YAAERLDKIRRQNRIKAELQAVLDEQ"
     gene            complement(3606..4466)
                     /locus_tag="ACHURS_003400"
     CDS             complement(3606..4466)
                     /locus_tag="ACHURS_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR1239049.1"
                     /translation="MKITDHKLSEGIALTFRVPEGNIKHPLIILCHGFCGIRNVLLPC
                     FANAFTEAGFATITFDYRGFGESDGERGRLVPAMQTEDIISVINWAEKQECIDNQRIG
                     LWGTSLGGGHVFSAAAQDQRVKCIVSQLAFADGDVLVTGEMNESERASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLSDNESKVFFEKVKGQYPEMDIKIPFLTVMETLQYKPAESAA
                     KVQCPVLIVIAGQDSVNPPEQGKALYDAVASGTKELYEEADACHYDIYEGAFFERVAA
                     VQTQWFKKHL"
     gene            complement(4525..5271)
                     /gene="traX"
                     /locus_tag="ACHURS_003401"
     CDS             complement(4525..5271)
                     /gene="traX"
                     /locus_tag="ACHURS_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFR1239050.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWFQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWHEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSYRLYRAEDRAERLALLACLLAVIPALNLASSDAAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPVFYACHLAVLGVLAL"
     gene            complement(5291..10561)
                     /gene="traI"
                     /locus_tag="ACHURS_003402"
     CDS             complement(5291..10561)
                     /gene="traI"
                     /locus_tag="ACHURS_003402"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HFR1239051.1"
                     /translation="MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQDPQLHTHV
                     VVANVTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEALGYETEVV
                     GKHGMWEMPGVPVEAFSSRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEIRMAE
                     WMQTLKETGFDIRAYRDAADQRAEIRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLQEGMVFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYDRLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPATLPVADSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQ
                     KAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFTELGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRLEGAWAPEHSVTEFSHRQEAKLAEAQQKAMLKGEAFPDI
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWEKNPDALALVDSVYHRIAGISKDDGLITLQDAEGNTRLIS
                     PREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKQMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPKSDREVMNAERLFSTARELRDV
                     AAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPMTTDDGN
                     GLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPGTITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAENKPDLPDGRTEQAVREIAGQERDRAAITEREAALPESVLRESQREREAVREV
                     ARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            complement(10561..>10838)
                     /locus_tag="ACHURS_003403"
     CDS             complement(10561..>10838)
                     /locus_tag="ACHURS_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="type IV secretion system coupling protein; similar
                     to F plasmid TraD; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HFR1239052.1"
                     /translation="SSVINDKKSDAGVSVPAGGIEQELKMKPEEEMEQQLPPGISESG
                     EVVDMAAYEAWQQENHPDIQQHMQRREEVNINVHRERGEDVEPGDDF"
BASE COUNT         2323 a         3342 c         2484 g         2689 t
ORIGIN      
        1 ttcgtaggcc agagttacct gcaccactgt atttcccctg tgaatattca gctcacataa
       61 ccgttccctg aatatcagtg aaaaacacaa caccgtggcg cacacggcga gcaacccgat
      121 aagggcatac ttcgtcataa actttgacct ctctgcaagg aagaggctac aatcagtagt
      181 gcaatgtact tcatgcagac ctcacgagtt aatggattaa caagtggggt ctttgcattt
      241 ctggtacctg cgtcttgcct gaaccagaaa ggctcagaca cccgccgcga taataccaca
      301 ttccctgttg ctgactatta cctgcattgc ccgcttttta ctacagaagc acgaaggcgt
      361 cactccagcc aataagtatg ctttttattt acatccattt ttaatccggt accgacgttt
      421 tcctccagtt aactcgtttt tcgtatgact catggcaata aaattttctt taccatatga
      481 caacatccca ataatatcaa agttgtcttt taatttgatg gcatcctttt cacttacagc
      541 gacaataagt tgtttaccaa attttctgac catcattgca aactgtgata ctgctgatcc
      601 accacctaaa tcaggccacc cagcacaatt aaaaatgatt gtctgctctt cactattcat
      661 tgcatccctg ataaaggaga cagcatcctc atttctctct acatctgaat cactgttaat
      721 ctgagtgaaa tcataatatt tagaacctgg tatagaatac cccttaaaaa acttatcaat
      781 gtgggaggca gagttcccaa caataagaat attgtgttca cgcttaatca tcaattctga
      841 tacatcatca tatttcatat attcaccatt attatataaa gtttgggtac aggtggttcc
      901 ggaatttact ctgtgaccac catttcccgc tttttattac agaaacgccg ggagcgggga
      961 aaacttgctt tttcccgttt ccggggttgg acaactgagc aacgcgaagg cgtcagctac
     1021 gatgttccgg ggactgctga tcagcccctg ttgtattcag ccattctccg gtcatctgca
     1081 gcagttttct ggcatcgtgg cttctgagca acggaatacc agcctgcctc agaacctctt
     1141 ccagaagaat atcccggcgc cggcgttccg gtcgtaaatg gctggcatca tccagctcta
     1201 ctgctgcaac aatactgaac gaacgccgtt caacgataac cacatcaaca tgccactgag
     1261 acaccatcct gaataactgc caccactggc gtgatcgtgg ccggatattc ccgttcagct
     1321 ggacgatatc cgcgacccgt acctgcggac acagatacca ccgcttcata tccacaattc
     1381 tgaacagtcc tttcataaaa ctgacttccc tttctgtcag cagcgtcttc tgacgttgcc
     1441 agtattcacc gccggacagt attcgctctg catcaccggt ccgtatacct gccgcatgca
     1501 actgcatcag caaaggagaa tgctgattta cacttttgag gataatccgg ataagtaacg
     1561 ctgcaataat cagaacaatg agaataaaaa taatcagctt cattgtttat ctgctcctgg
     1621 taattaatta ttctgaatta ttaccttaca tatttccata tctcttttgg tatcctgtca
     1681 tataactttc cggtgattaa ttccgctaac cggtggtctg ctgctgaatt aaatgattca
     1741 cgttcactgt ttaataaact gtctctgttt ttatgaataa ttttttccag cgtattgact
     1801 gatgaacatc gccgtaactg atacagccat tcctgttttg ttttcgccat ttttattccc
     1861 gctgagaaca gatgaatgat aagattgcca tcgctttatt tacggtgtct ccatatccag
     1921 gggggaaccg gatcagcatc tgaccagagc ccacgttttt gttgtctggc ttcgttctga
     1981 aggacaggca gcacgggatc tgtgttgtac tgctcatata cccaggcagc cccggcccgg
     2041 accataaact gattaacatt cattccgtct ggtgcataaa cctgcccgag aatacgacca
     2101 taacgatctc gctgaaaata tgtgacggta accgtttttc ctgcaaccag tgatttcatc
     2161 atgtccgttg accaccggcc ataatcctgt ttcttttccg gtgcatcaat atttatcagc
     2221 ctgatccgca ctgccttccg tgagtccatg acctcgatgg tatcaccgtc cagtacacga
     2281 acaacccgtc catgaatatc tgcacacaat accggccatg aaaagataaa taataccagt
     2341 ggaatatatt tcctcatgtt attctccata tcagataaat ggcacctgta tttcaggtaa
     2401 tcctgaatat cgcagcacac actgaataac accttatttt gtgaaattaa tcacaaaata
     2461 taaaatcacg acttaatatg gaaaaaactc tgtgctactc agaactgtcc ggtcatactt
     2521 tccgaccgga tattaaaaaa agcaaatctc atgcattatt cagccttctg aatccctgga
     2581 gtaatgaagg ctgctttttc gttacaccct tccccggcag cgccggtggc agtgttcagc
     2641 cctttttcgg gcaaactgct gccagtctgt ccggggagca gactgcgcag cccgtagcca
     2701 tgcctcagcg gctcccggcc agtcccccct gacttccagc cggacagccc ggcgtccgac
     2761 ctgccggtag cgccataccc cggagttttg cgaatcacgc cctgttccgt tcattttcct
     2821 tcccccttta atccaggccg gtgaatgtgt tataaatttt tataaccttc ttctgccggg
     2881 tatggaagaa ggtttcagag aagccttatt tttccgcctc ctttagtgtg aaggaggctt
     2941 ttttattgtt catcaagcac ggcctgaagt tctgccttta tccggttctg gcggcggatt
     3001 ttatccagac gctctgccgc atacacttct tcctcctgag aaatatgctc cgtcacatac
     3061 ccttccgtgt cataccggca ggcaccggct ttcatggcac acagatagct ttctgaacgg
     3121 gtgatggcct tcagcgccct gcgcagtttt ttatgcgaga gcgggatatt ccgctgcgcc
     3181 acgtcttcca gtaacacgtc ccggataccg caggccagaa gtcggggtgt gtcaccgtca
     3241 aataatcccg gccaccaggg tttcagggtg ttcacggcct catccagcga gggcaggttc
     3301 agataaatgg acagcgcctg tctggcctgc gctttttttg ctgtcagctc tgcttcccgg
     3361 gcagccttct ccgccagttt ctgctttttc accttccatt ttggtggcgt ggtgacattg
     3421 atgatggttt tccggctccg ggtcggtgtt tccccttccg ttttccgttt cagtgtcagt
     3481 accggtcgtt tctgctctgt catgattgcc ctgacctttg tgaaaatatg tcctccaggt
     3541 cgttgaaact actcactatg atattaagct gacctcaacg acctctgaca tattaatacc
     3601 gacaattaaa gatgtttttt aaaccattgc gtctgtactg ccgccacacg ttcaaaaaac
     3661 gcgccttcat aaatatcata atggcatgcg tcagcttcct cgtataattc cttagtacca
     3721 gaagcaacag catcatataa ggcttttccc tgttctggtg gattaacact atcctggccg
     3781 gcaataacaa tcaaaactgg gcactgtact ttggctgcgc tctctgccgg tttatattgc
     3841 aacgtctcca taaccgtaag gaaaggtatt tttatatcca tttcaggata ctgacctttt
     3901 accttctcga agaatacctt tgattcatta tcactcagta cccgggtaac ccccacaaac
     3961 atctctttcc cggtgttttt cttcttctct gccattttat tcagcgtgga cagaaaagac
     4021 gctctctccg attcattcat ctctccggta accagaacat caccgtcagc aaaagccagc
     4081 tggctgacaa tacattttac gcgttggtcc tgcgcagctg cactgaagac atgcccccct
     4141 cccagcgatg ttccccataa cccaatacgc tgattatcaa tacattcctg cttttctgcc
     4201 caattgataa ctgaaataat atcttcggtc tgcattgcag gaaccagacg ccctcgttct
     4261 ccatcactct caccgaatcc tcgataatca aaagtaatcg ttgcgaatcc tgcttctgtg
     4321 aaggcattcg caaagcaagg gagcagaaca ttcctgatac cgcaaaaccc atggcataag
     4381 ataatcaaag gatgttttat atttccttca ggaacacgaa atgtcagggc aataccttca
     4441 gagagtttgt gatccgtaat tttcataaat atcctgtgtt atgttgttta ttaataatca
     4501 gaatcagcgt cagtatcata ccagtcacaa cgccagaacg cccagcacag ccagatgaca
     4561 ggcgtagaat accgggaaaa aatccccggg ccagaaacgg ggtaatgatt ttcctgcaca
     4621 cgacaccaga ccaacggtca gcaccgtcat tacaagaccg gccaccgccg ctgcatcact
     4681 ggaggcaagg ttaagtgccg gaataacggc cagcaggcat gcaagcagcg ccagacgttc
     4741 cgctctgtct tccgcccggt acagtcggta actgaccgcc agcatcagca gcccggcaat
     4801 gccgtaactg gtgccggaca aaggccccca cagggccatc agaagaatgg ccgctgctgt
     4861 acgccaccca ctgcgcgttt cacaccacgt cagcacctgt gccgccactg caaaggcaaa
     4921 caggatattc ccctcatgcc agggaaagcc ggccagataa tatgcgaact gggcaataat
     4981 tccccatccc cacagccggt taatggccgg ttgccggata tgcgcatgac gggacagatt
     5041 cagcccccac accagggcaa acagcggaaa ggctcctcgc ccggccagaa acatccattc
     5101 ctgcttcagc tggaatatca gattgatgtg gtccagtacc atcagcacca gcgccaccgt
     5161 tttgatgata tcccgctgtc cgggtgacca gaccagaaat gactgaaacc aggtatcggt
     5221 ccgtgcagcc agtgaatcgt tacgtgtcgt gttcgtgtta tccgttgtca tcagcgttta
     5281 tcttcctgta tcagtctccg cccagggttt tctctttctg caggtcacgg accatatccc
     5341 gctccatctg ctgcagacgc tcctgcagca gattttcccg ggcaacctcc cggaccgctt
     5401 cccgctcccg ttgtgattca cgcagcacac tctccggcag cgcggcttcc cgttcagtta
     5461 tggcagcccg gtcacgctcc tgcccggcaa tctccctgac agcctgctct gttctgccat
     5521 ccggcaggtc aggtttgttc tctgccattt tacggacaat ttcatctgcg cgacgttccg
     5581 tttcacggcg gatggcctct tcagcctgcc gctgtgccag cacttcagct gtaaccggtt
     5641 caccatttcc ggcccccggc tggacgctgt tgtccgggat atccccccac acgcgaccac
     5701 cggtgatggt accgggattc cacggacgac cttcaccggc gattcttacc accacaccgc
     5761 tgtcaggatt atcacgggca atccggacac catcctgcat attatcagcc agcaggctct
     5821 ctccgttacg gctgccctgc agggccacga actgcgcatc cccgctgcct ttcacccggc
     5881 cttcaccact gaagccccgc agcccgtttc cgtcatccgt ggtcatcggg ttcagccaga
     5941 tacctgctga cttgccgtta cggtcaaaag ccggcagtgc cacatacggc tgcggatatt
     6001 tgcgcccggg tgcaataaac cgtgcaggac tgtctccccc ggccagccct gcctgacgga
     6061 gaacggcacg gccggccgcc acgtcccgca gctcccgcgc cgtactgaac agccgctctg
     6121 cattcatgac ctcccggtcc gatttcggct caagcacatc gtgggcagtt cctttctgta
     6181 cggcattgtt aatggcatcc gtccagccct gacggttatc ggtatacacc tgcacatgct
     6241 gcttcatacg cgacagggcc acgtaggctg actcaaagcc ggccatctgt ttccggttac
     6301 cttccgtgcc ttcaagcgca atggcaaagg tttcacttgc cccctgcgca ccgtgggcgg
     6361 tgatggcata ggccaggtca atatgttgct ctgcccgctc ctgaccgggg cgaatcaccc
     6421 gggtctgctg tccgtccgac agcgtgacac tgtcaccgga aaccgctgtc accgtccaga
     6481 cgctgttggc cacataaccg cgctcccggt cactcttcgt gaagcgcatc cggtcaccgg
     6541 ttcccacccg gatggtgtcc ggagtgtaca gtgtgacgcc ttcagccacc gcctcccggg
     6601 gcgaaatcag ccgcgtgtta ccttccgcat cctgcagggt tatcagcccg tcatccttgc
     6661 tgataccggc aatccggtga tacacactat ccaccagggc aagggcatcc gggtttttct
     6721 cccaggtgga gagacgacgc agttctccgt cacgtatatt cgctgtgttc aggacaggca
     6781 ccatgacctg ctctttgccc agctcaccgg ccttttcccg tgcatcatga atcatgctgt
     6841 tcagtacgcg ccggtcctca ttcaggtgcg tgacaatcag cgtctgctcc cgtgcttccg
     6901 gtgtcctgcc ggtatagtcg cggacaatgg cctcatacag tgtcatgggg atatccggga
     6961 aagcctcgcc tttcagcatc gccttctgct gcgcttctgc cagtttcgct tcctgtctgt
     7021 gactgaactc cgtcacggag tgctccggtg cccatgcgcc ctccagacgt ggcacctgag
     7081 acggtttcac actctcaagc ccggacagtg ccctttccac atcccggtta atcaggctgt
     7141 ataccgcctc ccgcagttcc ggcgtctgac gcacaatctc cttcatgatg gccacatcgg
     7201 cagcactgcg cgtctgctgg agacggaaag gctgaccggg cgcgatggcc tgcagctggt
     7261 ccgtgtcacc gctggccaca gcacgaccgc caccggccgc aatcagggcg tatgcccgtg
     7321 ccatgtcggt attgcccacc attgagctct catcgagcag aaacagcgtg ttgctgaaat
     7381 ccggcgtttc tccgctgcgc tgctgcagct gcgtgtcatg cagaaaggac gccagtgtct
     7441 gtgcatccac gccggcgctg cgcatctcac cgaccgcacg gtgcgtgggc cccagcccca
     7501 caacccgggg acgctcactc tccggcagca tgttcacggc tgacatcacc gcccggaact
     7561 gagtggtctt acccacaccg gcatacccct gtaccaccgt gaaacggtcg gacgtttcca
     7621 gtatcatacg ggtggcggca cgctgtcccg acgttaacgt ctccatgagt tcgccgggta
     7681 ctctctccat cagcggcgtg accgcctcct tgccttcgag aatatggcga agaatgctct
     7741 tttctgcctc atacgacgca cgggaaacca gcaggcctgt gccatagcct tttgccacat
     7801 ccacatacag taagtcaccg cgttttatct gcgcattgat ttcccctccc agttcagtaa
     7861 aaccggttcc ttctgcagca aacgacttcg cctctgtcag caggtccacc atgctgaagg
     7921 ccaggttttt actttccagc acaggaaggg ccagatgaat ggcctgctgt gccggcgtgt
     7981 gaagcccggc tttctgcaga ctgatagcgg tttccagcaa tgtctcaccg gcacgcgcct
     8041 ttatctgctc agaaaccacc gtaaaggacg gatggcgggc gagtttttcc gcagtacggg
     8101 tttcatccag tgaggaatac agccggacat cacgaccgct gcgggccaga ccgttcaggg
     8161 tggcgttgtc cattgccatc tgtgtgacgg aggcaaacac cgtcgcactg tcactgacgg
     8221 aatgcccggg cgtttccacc cagccgttct ccagcttcag tgccgtgaac ggtgaatcag
     8281 ccacaggcag ggtggccggt tcagcccgcc ccggcacaac aaccgtcatc gcatcttcac
     8341 tgacggatgc cacctgcagg cggtcaccgc cggagacgcg gagtccggga attttccctg
     8401 tcaccctcag tcgctcgccg tctgccaccg gcattttttc cggccggaac agcgaccagc
     8461 tgctgtccag ggaggaaata cgcaccacct gcgtttcgcc ctgcgcatcc cgcagggtca
     8521 ggctgtgact ctgcgccgtc acccggtcga taacatagcg gtcatgactg cgtgtctccg
     8581 ggttccactg ctccatcacc atccccggtc ggtacatatc ccgcagataa cggctccggc
     8641 tgtccagcca gaccggtgac agggcggtca tggtcacctc cgggtgtccg agcacgccct
     8701 gtgttttcag ctcactgcga atggcctgtg tcagtatggc ctgttcccgt accccgctga
     8761 cctgtgccac gctctcttct ccggctttca cgctggccgc aaaatctccg gccagccggt
     8821 catagcggac attccggtcc ggttcactga tgatggttgc cggtcgctgt tctccgccct
     8881 gccagcgata cgtgttcacc ccggcatcct tcatggccat cagcgcactg cctgtaccgg
     8941 ttcgctgccc gctgtcggtt atcaggacct gtacgttatg acgtgcggca ccgtccagca
     9001 gggttaacgt ctctttcagg gagagttttt cgccctggtc gacgataaca gtactgcccg
     9061 gcgtgaagac catgccctcc tgcagctgac gacgtccggt tatcagctca ccggacagcc
     9121 gttcatcctg cttcaggttc atctgcgagc gacggtcagc agcgataatc tgcacctccc
     9181 gcccctgctc ccgggccatc atgaccagtt cagccacccg ctcacgctgc ccggctgcac
     9241 cgccctgccc ggacacaatg gccagcgacg gacggtcctg tgccagaaca ctgaccgcat
     9301 cgctgtaacc agccgtccgg gggacacttt tctccggatg tacggtcacc cggttctgtt
     9361 tcatgatgtc acgactgagt gcccggacgg acagctcatc gagcacatga attcctgatg
     9421 tgaacagccc cttctcacgg tcgaggggga taagctgctc acggctgatg gcctcatcga
     9481 taccggcgcg tgcccgttca atcacaccat tttccggcgg cagtatgccg accgtcctgg
     9541 ccagcacgtc cgtatacgtg aactgcactt ttcgttcact taatccggca atcgcctgtg
     9601 tcaccgcctg ctgcacatcc ggcccgtcct gtgaagcagg cccgggcgcc tgcgtgcgga
     9661 tctccgcacg ctgatccgcc gcgtcacgat atgcccggat gtcgaacccg gtttccttca
     9721 gcgtctgcat ccattcagcc attctgattt ccggatcgac gtgctgtttg gattttcgcg
     9781 tatccagtgc cgccacatcc cgggatttca gcgaggcgtc ttcaccgacg gcctccctga
     9841 tagcctgtga acgactggaa aaggcctcca ccggtacgcc cggcatctcc cacataccgt
     9901 gcttaccgac cacttcggtt tcataaccca gcgcctcaac gcgctgacgt aactcactct
     9961 gatatatttt cccgaacgca atccggttcg ctagcacgtt ctcggaaaat ccggttttcc
    10021 ccactttgtc actgctcagt gtcttccact cgccgttatg ctgcgtgaca ttagccacca
    10081 ctacatgcgt gtgtaactgt gggtcctgat cgcgactggt gtcatggtta aacagtgcca
    10141 tcaccagatt accggtcagc accgtttctg actgtccgtc cgtcatcacc cgtgtggaag
    10201 ccagcgcctc cacctgacgg acagcaaaat ccacggcctg gttatgtgca tcaataagac
    10261 gtttatcccc cccgagcatg gccatcatgg agacactttt gggggcggag aaggtcaggt
    10321 cgtagccggg acgatgctta ttactgccat cctgcatgcg gcttaaatcc gccccgtccg
    10381 gcagcctgcc ctccagaaga cgggtaaaaa catccttatc gacgctgccc tgcagcccaa
    10441 gctgttcagc cccctgacca gcccagcgtt ctcccatgct gcccagcaca tagtaattat
    10501 ccttgtcggt gtaatagttt ccggcactgc ctgccgattt aacaacgctg aaactcaaca
    10561 tcagaaatca tctcccggct caacatcctc cccgcgctcc cggtgcacat taatgttcac
    10621 ctcttcacga cgctgcatat gctgctggat gtccggatga ttttcctgtt gccatgcctc
    10681 ataagcggcc atatccacca cttcaccgga ttcactgatc ccgggtggca gttgctgttc
    10741 catctcttct tccggtttca ttttcagctc ctgctcgatc ccccctgccg gaacactcac
    10801 acctgcatct gacttcttat cattgatgac agaagaca
//
LOCUS       DAZPDU010000148        10832 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZPDU010000148 DAZPDU010000000
VERSION     DAZPDU010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10832
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.150"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..277)
                     /locus_tag="ACHURS_003404"
     CDS             complement(<1..277)
                     /locus_tag="ACHURS_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1239053.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRS"
     gene            complement(444..1184)
                     /locus_tag="ACHURS_003405"
     CDS             complement(444..1184)
                     /locus_tag="ACHURS_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFR1239054.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            complement(1277..1912)
                     /gene="argO"
                     /locus_tag="ACHURS_003406"
     CDS             complement(1277..1912)
                     /gene="argO"
                     /locus_tag="ACHURS_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFR1239055.1"
                     /translation="MFSYYFQGIALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            complement(2051..2911)
                     /gene="mscS"
                     /locus_tag="ACHURS_003407"
     CDS             complement(2051..2911)
                     /gene="mscS"
                     /locus_tag="ACHURS_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFR1239056.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            complement(3102..4181)
                     /gene="fbaA"
                     /locus_tag="ACHURS_003408"
     CDS             complement(3102..4181)
                     /gene="fbaA"
                     /locus_tag="ACHURS_003408"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFR1239057.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(4396..5559)
                     /gene="pgk"
                     /locus_tag="ACHURS_003409"
     CDS             complement(4396..5559)
                     /gene="pgk"
                     /locus_tag="ACHURS_003409"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFR1239058.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETASATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(5609..6628)
                     /gene="epd"
                     /locus_tag="ACHURS_003410"
     CDS             complement(5609..6628)
                     /gene="epd"
                     /locus_tag="ACHURS_003410"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFR1239059.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7000..7431
                     /locus_tag="ACHURS_003411"
     CDS             7000..7431
                     /locus_tag="ACHURS_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFR1239060.1"
                     /translation="MMTLPYPYSSPARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            7454..8032
                     /locus_tag="ACHURS_003412"
     CDS             7454..8032
                     /locus_tag="ACHURS_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFR1239061.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            8033..8740
                     /locus_tag="ACHURS_003413"
     CDS             8033..8740
                     /locus_tag="ACHURS_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFR1239062.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVAWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWTHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIILLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            8728..9405
                     /locus_tag="ACHURS_003414"
     CDS             8728..9405
                     /locus_tag="ACHURS_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1239063.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAETEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWALERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            9399..10076
                     /locus_tag="ACHURS_003415"
     CDS             9399..10076
                     /locus_tag="ACHURS_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1239064.1"
                     /translation="MLTLNQISYRWPSAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(10048..10761)
                     /locus_tag="ACHURS_003416"
     CDS             complement(10048..10761)
                     /locus_tag="ACHURS_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFR1239065.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLVQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(10758..>10832)
                     /locus_tag="ACHURS_003417"
     CDS             complement(10758..>10832)
                     /locus_tag="ACHURS_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000209973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1239066.1"
                     /translation="ARFSGMVKTALTLAVTTTLKELTP"
BASE COUNT         2580 a         2878 c         2699 g         2675 t
ORIGIN      
        1 gtgaacgacg ccctgagtta tggatttcat tatcaatcgc agaaagtcgt tgataaaccg
       61 ggaacagttt ttgatacagt tcctgaccgg ctttattgag gctgatatta ttatctttgc
      121 gggtaaatag cgtatagcca atttgttctt ccagcgcggc aatgctttta ccaaatggcg
      181 aagccgtcat atggattttt tctgccgccc gggcaatatt attggtttgc gctaataaaa
      241 tgaaattgcg catctttttc gaaataaaaa tgtccatccc ccctccccgc atccttgtcc
      301 gcttaaccgt ttcagtcagc ctatccttga tgaaaccgcg agcaaagata ggtgattacg
      361 tcatggtttt acagaaaatt acagaaaaag gaggcaatat cgggtaaagg cattagcccg
      421 acgaatacgt cgggctacaa gtattattgt gctgcaggtg ttttagcggg ttgttgatcc
      481 acaggttcta actggaagac cacatcgacc tgatcatcaa actgaatagc ggcctgctcg
      541 taagtttcct gggcagacac cggcgcggca tcggctttca tcatccgcac cattgggctg
      601 ggctgatagt tggaaacatg gtagcgcacg ctatataccg gccccagttt acgatgaaag
      661 ccgttcgcca gttcctgcgc ctgatgaatc gcgttatcaa tcgctgcctt acgcgctttg
      721 tctttatagg catccggctg cgccacgccc agcgacacag aacgaatttc gttaagaccc
      781 gccttcagcg cgccatccag caaggaattc agtttgtcta actgacggag cgtgacttcc
      841 accgttctca cagcgcggta gcctttcagg atacttttac catcctgata atcataatct
      901 ggctgggtgc gtaagttcgc tgagctgata tcttttttcg cgatctgatt gagttcaagg
      961 aaggaaatgt attgtgcgac gcgctcatct gcctgtttct tggctgtagc ggcatccttc
     1021 gcggccacgt taacttcaat cgcaagagtg gcaatgtctg gcaccgcatc cacacttgcc
     1081 gtaccggagg tgacaatatg cggtccatcc ggcaattcgt tagcctgcgc tgccatcccg
     1141 ctaataccca ttaatgccgc cagggcgata actttgaact tcacgttagt ctctccgtgt
     1201 taattgactt gcccaaaatt caggacctct ggaggcaagc ttagcgcctc tgttttattt
     1261 ttccatcaga tagcgcctaa ctgaacaagg cttgtgcatg agcaataccg tctctcgcca
     1321 gctgcaaggc tataaaccac atgacacatc ccactaccag attgataatg cgctgtgatt
     1381 ttgccgtgcg caggcgcggt gccagccagg ctgcgagaat agccagacca aagaaccaca
     1441 ggaaagaggc gctaattgtc ccgagcgcaa accaacgttt tggttcgacg tcaagctgcc
     1501 cgccaaggct gcccagtaca acaaaagtat ccaggtaaac atgcggattc agccaggtca
     1561 ctgccagcat ggtggcgata attttccatc tgccttgctt caggacttcg gcactagcta
     1621 actcaatatt actactcatc gctgttttaa aagcgccaaa accataccac agcaagaagg
     1681 ctacgccgcc ccaggtgacc agcgccagca accacggcga ctgcatcaat aacgcgctgc
     1741 caccaaaaat cccggcgcaa atcaggacca aatcgctgat agcacaaagt aaggcaatca
     1801 taatgtggta ctgacgacga atgccctgat tcatcacaaa agcattttgt ggaccgagcg
     1861 gcagaatcat agctgcccca agagcaatac cttgaaagta ataagaaaac acgttggata
     1921 ttccgaatta agtatctgat gcagagacta tatcgcggga ttgttattag cggaaattga
     1981 taatattaat tggcaatcag aataactaat aagagagagg ccagagagta ttatctggcc
     2041 tcagcgttga ttacgcagct ttgtcttctt tcacccgctt aaagttcaca tccatttgcg
     2101 ggtacgggaa gctgataccg gcggcatcaa attcacgttt aatacgctcc agcacatccc
     2161 agtacacgtt ttgcagatcg ccgctgttgc tccagacgcg gaccacgaaa ttaatcgacg
     2221 atgcaccaag ttcgttcaga cgcacagtca tttcgcgatc tttcaaaatg cgatcttcag
     2281 actggataat attggtcagg atctgcttaa cctgatcgat atcggaatca tacgccacgc
     2341 caataataaa ttcgttacgg cgaactggct cgcgagagaa gttaataata tttccggcaa
     2401 taattttacc gttcggaata acgataattt taccgtctgc agtacgcatg gtggtggaga
     2461 aaatctgcac actcagcaca gtaccggcta cgccgccgag atcaacatat tctccggcac
     2521 ggaacgggcg gaacatgaca agtaacacgc cagcggccag gttagaaagt gacccctgca
     2581 aagccagacc aacagctaag cctgcggcac cgagtacagc aatgactgac gctgtttgta
     2641 cacccacgcg tcccagggca gcgattagcg taaaggcgat aataccgtaa cggactaatg
     2701 cagaaagaaa atcagcaaca gtggcatcga ttttacggga gatcatcagg cgattcaccg
     2761 cgttggaaat catccgcgcg ataatcaaac caacgatgat gatcgcgagt gccgccacga
     2821 tgtttactgc ataacttagc agcagcgcct ggttagctac cagccagctt cccgcgccgt
     2881 ttatgctatc gacaacattc aaatcttcca ttcaatattc cttttcaact gactcaaaat
     2941 ggagaaatac actccgccgc cttatgacgg gcagtctgac aagggtaaac aataaagcat
     3001 aattttgcca aatagatcac agatttctca tctgaatcac cttgcttttg gcgaaaaaaa
     3061 gacccgcaga gcgggccttg agataagcag aaaggaatat cttacagaac gtcgatcgcg
     3121 ttcagttcct ggaatgcttt ttccagacga gcgatcatcg aagtctgacc ggcacgcagc
     3181 catacgcgcg gatcgtagta tttcttgttc ggctgatctt cgcctttcgg gttacccaac
     3241 tgcccctgca gataagcttc gttcgctttg tagtagttca gaacgccttc ccaggttgcc
     3301 cattgggtat cggtatcgat gttcattttt actacgccgt agcttacgga gtctttgatt
     3361 tcctgagcag tagaaccgga accaccgtgg aatacgaagt tcaggctgtt gtgcggcagg
     3421 ttgtgtttct tggaaacata ttcctgagaa tcacgcagga tggtcggagt cagaaccacg
     3481 ttacccggct tgtaaacacc gtgtacgtta ccgaaggacg cagcgatggt gaaacgcggg
     3541 ctgattttgc tcagttcggt gtatgcgtaa tcaacgtctt ccggctgggt gtacagtgca
     3601 gaagcgtcca tgtggctgtt gtccacgccg tcttcttcac caccggtgca acccagttcg
     3661 atttccagag tcatgccgat tttggacatg cgctccaggt atttagagca gatttcgatg
     3721 ttctcttgca gagattcttc agacaggtcg atcatgtgag aagagaacag cggcttaccg
     3781 gtagctgcga agtgtttttc acccgcgtcc agcagaccgt cgatccacgg cagcagtttc
     3841 ttcgcgcagt ggtcagtgtg caggataacc ggaacaccat aatgttcagc catctggtga
     3901 acgtgatgcg caccagagat cgcgcccagg atagcagcac cctgcggaac gtcagatttc
     3961 acgcctttac cagcgataaa ggaagcacca ccgttggaga actgaacgat aaccggcgct
     4021 ttaactttag cagcggtttc cagtacggcg ttgatggagt cagtaccgac gcagtttact
     4081 gctggcagtg cgaagttgtt ttcttttgct acctggaaaa ctttctgtac gtcatcacca
     4141 gtgattacgc caggttttac gaaatcaaaa atcttagaca tgtctcttgt cctgtatcgt
     4201 cgggccttat actcatcata cttcaagttg catgtgctgc gtctgcgttc gctcacccca
     4261 gtcacttact tatgtaagct cctggggatt cactctcttg tcgccttcct gcaactcgaa
     4321 ttatttagag tatgaaaaaa tgcgtgctct aaaagcgcgc tgaaacaagg gcaggtttcc
     4381 ctgccctgtg attttttact tcttagcgcg ctcttcgagc atcgctactg caggcagtac
     4441 tttaccttcc acgaattcga ggaatgcgcc gccgccagtg gagatgtagg agattttgtc
     4501 agcaatgccg aacaggtcga ttgctgccag agtgtcgccg ccgccagcga tggagaacgc
     4561 ttcgctgtct gcgatagcgt tagccacgat ttcagtacct ttgcggaagt tcgggaattc
     4621 gaacacgcca accggaccgt tccacagaat ggttttcgca ttcttcagga tttcagccag
     4681 ttcctgagcg gaagcatcac cgatatccag gatctgctcg tcagctttca catcgttaac
     4741 agatttcagg gtagccgatg cagtttcaga gaactcggtt gctacgcgaa catcagacgg
     4801 aaccgggatg ttgcaggtgg tcagcagacg tttagcttcg tcaaccaggt cagcttcgta
     4861 cagggattta cccacatcgt ggccttgtgc cgcgataaag gtgttagcga taccaccacc
     4921 aacaatcagc tggtcagcga ttttagacag ggagtccaga acggtcagtt tggtagatac
     4981 tttagaacca ccaacgatag ccaccatcgg gcgagcaggt tctttcagtg ctttacccag
     5041 cgcgtccagt tcagctgcca acagcgggcc tgcgcacgca acatcagcga atttaccgat
     5101 accgtgagta gaagcctgcg cgcggtgagc agtaccgaat gcgtccatta cgaacacgtc
     5161 acacagtgca gcgtattttt tggacagggt ttcgtcgtct ttcttctcgc ctttgttgaa
     5221 gcgaacgttt tccagaacaa ccagttcacc ttcagcaacg tcaacgccgt cgaggtaatc
     5281 tttaaccaga cgaaccgggt tagacagttt gtctttcagg tagttaacaa ccggcagcag
     5341 agagaattct tcgttgtact cgccttcggt aggacgaccc aggtgggaag ttaccatcac
     5401 tttcgcgcct tgtttcaggg ccagttcgat ggtcggcaga gaagcacgga tacgcgcgtc
     5461 gctggttact ttcccgtctt ttactggtac gttcagatcc gcacggataa atacacgttt
     5521 cccagcaaga tccagatcgg tcatcttaat tacagacatg gtgaatcctc tcgttgattc
     5581 taaaagtttt gcagacgctg cttgcgtctt acctgaaagc aacagtagcc atagctaacg
     5641 tcgtgtcgag cattcggtta gcaaagcccc attcgttatc gcaccagacc aacgttttga
     5701 tcaggtgtgc gccactgacc cgggtttggg tgccatcgac aatggcactg tgcggatcgt
     5761 ggttaaaatc tacagagacc aacggcaatt ccgtatagtc aactatacca tgaaatgcac
     5821 cttgtgctgc tttttgcagc aacaggttga cttcattggc ttttacaggt ttcttcaccg
     5881 tcacgcttaa atcgattgcc gtcacattta tggttggcac acgtaccgca atcgcttcaa
     5941 agcgatcgtt aaattgcgga aaaaatcgtg tgataccggc ggccagttta gtatcgaccg
     6001 gaatgatcga ctggctggct gcccgggtgc gacgcaggtc aggatggtat gcatcaataa
     6061 cctgttgatc gtgcatggcg gagtgaattg tggtcacagt gccggactca ataccgtacg
     6121 catcatctaa caatttgatg acgggaatta tgcaattcgt ggtacaggaa gcgttagaaa
     6181 cgatgcggtg ttccgcacga agttgatcct gattgacgcc gtaaacaacg gtcgcgtcga
     6241 gatcgttact gccaggatgt gaaaagagca cttttttggc cccggcggca atatgcgctt
     6301 cgccatgctc gcgggagcca tatacgccgg tgcagtcgag gactacatca acgccaagtt
     6361 cacgccaggg gagcgattgc agtgaacgtt catgcaatac gcggatggcg tcatcaccaa
     6421 caaaaagttg atcgcgttcc tgtcgtactt cccatgcaaa acggccatgg ctggtgtcat
     6481 atttcaacaa atgcgccatg cccgcagcat ccgccagttc gttgattgcc accacggtaa
     6541 tttccgcccg gcgtccggat tcatacaaag cacgaaccac attacgcccg atgcgaccga
     6601 agccatttat cgctacgcgt acggtcatag atctcctgca aggttttccc tgagcaaatt
     6661 tgccagacag agtaatccag caaatcgtcc ggggaaacct tacctgtcgc aaactgcgac
     6721 tgattggtta attgtcgaac atttaatcga ctgaaacgct tcagctagga taagcgaaac
     6781 gtggaataaa aggaatgatt gtccagtcaa agaaaacaat tatctgactt acgtcacgtt
     6841 ttcagccccg cccctgtgga atttattcca ctttgcagaa tcacgaataa gcgtagcaaa
     6901 atcttttcgt tgtgccgtca caaaccctga cttatatcag aatatgactg atgcagcgtc
     6961 gatattatcc gcaatacgtc cgagtttcgg agtactccaa tgatgacatt gccctaccca
     7021 tattcttccc ccgcacgctt ttatgcactg cgcctcctcc ccgggcagga agttttttcc
     7081 cagctacacg cttttgcaca gcagcatcaa cttcatgccg cctggattgc aggctgtacc
     7141 ggtagcctga ctgacgtcgc cttgcgctat gccgggcaag aaggcaccac gctccttaac
     7201 ggtacattcg aagtgatctc actaaacggc acgctggaac agagtggtga gcatcttcat
     7261 ctctgcgttt ccgatccaca cggcacgatg cttggcggtc atatgatgcc cggctgtacc
     7321 gtgcggacca cgcttgaact ggtgatcggt agccttgagg aactggcgtt tagccgccaa
     7381 ccttgcgcgc tttctggtta cgacgagttg catatttccc ccgttaaata actactttca
     7441 aagaggtttt cttatggcgc gtagccactt ttccagtcag gcactggtgt tgatcgttat
     7501 atctatcgcc atcaatatga tcggcgggca actggccagc atggtcaaat tacctatatt
     7561 tctcgactcc atcggcacac ttattagcgc ggtactgctt ggtccggtga tcggaatgct
     7621 gaccggctta ctgaccaatt tactctgggg attgctgacc gacccgatcg ccgccgcatt
     7681 tgcacccgtc gcgatggtca tcggcctggt cgccggttgg ctggcgcgag cgggttggtt
     7741 tcgcaccctg ccaaaagtcg ttgtcagcgg cgtgattatt acgcttgcgg taacggtcgt
     7801 cgctgtaccg ctgcgtactg cgctgttcgg cggtgtcacc ggtagcggag ccgatttgtt
     7861 cgttgcctgg atgcactcga tggggcaaaa cctggtggag tcggtggcga ttacggtgat
     7921 aggcgcgaac ctggtcgata aaattcttac agcggtgatt gtctggcttt tactgcgcca
     7981 gctgccgatt cgcaccacgc gccattttcc ggcaatggct gccgtgcgct aaatgcatcc
     8041 atttacctca ttaacactgt gggcattggc ggcctgcacc acgcttatcc tgcccgcgca
     8101 gaccattttg ccgatctata gcgcggcgac ctttttttgt cttattgcgc taaaggccac
     8161 gcggcggcgg gcaaaatatg tcgcctggct gatgttctcg ctgggagccg ggctgtggct
     8221 ggtacacggt ggctggctaa cagaatggct aagcggcacg ccgcgttcgc cggagcgttg
     8281 gacgcatgct attacgctct ggttgcggat tctggcgatt gtttcaacct cacagttgtg
     8341 gatgcagtac gtccccgtgc aatgttttat ccgcgctctg tttgcttctc gcctgccgcc
     8401 aggcgtcgct tatctgtttg ccggaccgtt attggtcgtt gagcagttaa aacggcaatt
     8461 agcgattatt catgaagcgc agcgtgcccg cggcgtgccg ctggatgagg gctggtatca
     8521 gcgtttacgt gcgatgcccg cgttaattat tctgctaaca cacaatgcgc ttaacgatct
     8581 ggctgtccgt ggtgcggcgc tggatatgcg ggcatttcgg atccacaacc gacgaacaac
     8641 gttgtgggca ccagccgata gcaccctaca acgcgtagcg cgttacacca tgatcttgct
     8701 gatgctaaca gaattcggag cgtggatatg gttacgttag aacagttccg ttattgcccg
     8761 acacactcaa cgcatcctcc gttctgttat gactttcact atgtcaaacc ggggatggtc
     8821 gccatctttg gtgacaacgg cagtggcaaa agtacgctgg cacagctgat ggcgggctgg
     8881 tatccggact ttctgccagg agagataaca ggcacgggga cgctcctcgg tacgcccatt
     8941 gggcatctcc cgcttaatga acaatccgcc accatccagc tcgtccagca atcgccctat
     9001 ttgcagctct ccggctgtac ctttagcgtg gaagaagaag ttgcctttgg gccagaaaat
     9061 ctgtgtcttg ctgaaacaga gattatggcg cggattgatg ccgccctgac tctgaccgag
     9121 tgccagccgt tgcgccatcg tcacccggca acactctccg gtggagagac acaacgcgtg
     9181 gtcatcgcct gcgccatcgc gatgcagcca aagctcttaa ttcttgatga agcctttagt
     9241 cgcctgacgc cgcaggccag cgaaatgctg ttgcagcgct tacaacactg ggcgcttgaa
     9301 cgtggttcgc tgattattct ttttgaacgt catcgcacac ctttcctgaa ctactgccag
     9361 caagcgtggc aattgcaaaa cggagcgctc cagccactat gttaacgtta aatcagatat
     9421 cttatcgctg gcctagcgca gcaacggatt gtctttgcga catttctctg caactcaaac
     9481 aaggtgaatg gttggcgctg accggcgaca atggcgcggg aaaatcgacg ctgttacgag
     9541 taatggcagg tcttcttacg cccacagccg gtacggttat gctgcaacaa caggcgatga
     9601 aaaacctgaa aaaccgccag cgcgcggcaa aggttggcgt actgtttcag gaagcggaaa
     9661 accaactttt tcacagcacc gtcgctgacg agatcgcatt tggtttgaaa ctgcaaaaat
     9721 gcccggcgga tgaaatcact caacgtaccc atgctgccct gcaatgttgt cagttagcag
     9781 acactgccaa ctcgcacccg ttggatctgc atagtgcgca gcgacggatg gtcgccgtcg
     9841 cctgcctgga agcactctct ccgccactat tactgctgga cgagccgagt cgggattttg
     9901 acgaaaattg gttgagcgtg tttgaaagct ggctggagaa gtgccgccag cgaggcacga
     9961 gcgtggtagc aatcagtcat gacgccgcat ttacccggcg tcatttctct cgtgtggtgc
    10021 gactggaaga tggtgtaatc aggaacgtta atccaccaga cgatattcac ccgtagcggt
    10081 catcattaag gtcaaatttg caggcagact ctcttccagc acccggcgaa cattcggccc
    10141 gtcggtacgg tcataaaagg cttctgcatc tgccagcgat acccctcccg ccagcttgcg
    10201 ccccaccaga cgctcccgca acgcagtagc tggcgcgttg ataaagatcg agaaatcaca
    10261 aaattgcgca agctggcacc acttctcgtc gtccagcaac aaccagtttc cttcgacgat
    10321 gacgagtggt gcggtaacgt gcaacgcatc ttcaacagga tcatgctttt gtcgatcgta
    10381 ctgcggccac gtacaatccc cttccaccac ctggcgcaga ttttccgcca gtttcgcaac
    10441 gtcaaatgtc tctggtgcgc ctttgaaggg gcgcaattga tgcgcgtcca gccagctatt
    10501 gtaatggtga aaaccatcca tcgggagcgt ctgaatagcg ggcagctccg ggtcttgttg
    10561 cacgagatat tcccagaagg tggttagggt agatttaccc gtccccggcg gagcgcaaag
    10621 aaaaaccacc gtacgccgct gcggatttac cgtctgtaag gccgccagca tacgcagcaa
    10681 cggtttatgc acgcgttcta tctcttcatc atggtactgc gcttgcacgt tcagcccatt
    10741 caccgttaat tcaattttca cggcgttaat tcctttaagg tggtcgttac tgccagcgtc
    10801 agcgccgttt tgaccattcc gctaaaacgc gc
//
LOCUS       DAZPDU010000149        10812 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZPDU010000149 DAZPDU010000000
VERSION     DAZPDU010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10812
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.151"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..696)
                     /locus_tag="ACHURS_003418"
     CDS             complement(<1..696)
                     /locus_tag="ACHURS_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR1239067.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAG"
     gene            952..1107
                     /gene="rseD"
                     /locus_tag="ACHURS_003419"
     CDS             952..1107
                     /gene="rseD"
                     /locus_tag="ACHURS_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFR1239068.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            1104..1679
                     /gene="rpoE"
                     /locus_tag="ACHURS_003420"
     CDS             1104..1679
                     /gene="rpoE"
                     /locus_tag="ACHURS_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFR1239069.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            1712..2362
                     /gene="rseA"
                     /locus_tag="ACHURS_003421"
     CDS             1712..2362
                     /gene="rseA"
                     /locus_tag="ACHURS_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFR1239070.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWHKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGLQTLGTQSQ"
     gene            2362..3318
                     /gene="rseB"
                     /locus_tag="ACHURS_003422"
     CDS             2362..3318
                     /gene="rseB"
                     /locus_tag="ACHURS_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFR1239071.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVISFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            3315..3794
                     /gene="rseC"
                     /locus_tag="ACHURS_003423"
     CDS             3315..3794
                     /gene="rseC"
                     /locus_tag="ACHURS_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFR1239072.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPDLVRFETSSEDASQ"
     gene            3992..5791
                     /gene="lepA"
                     /locus_tag="ACHURS_003424"
     CDS             3992..5791
                     /gene="lepA"
                     /locus_tag="ACHURS_003424"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFR1239073.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            5807..6781
                     /gene="lepB"
                     /locus_tag="ACHURS_003425"
     CDS             5807..6781
                     /gene="lepB"
                     /locus_tag="ACHURS_003425"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFR1239074.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            7054..7734
                     /gene="rnc"
                     /locus_tag="ACHURS_003426"
     CDS             7054..7734
                     /gene="rnc"
                     /locus_tag="ACHURS_003426"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFR1239075.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            7731..8636
                     /gene="era"
                     /locus_tag="ACHURS_003427"
     CDS             7731..8636
                     /gene="era"
                     /locus_tag="ACHURS_003427"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFR1239076.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            8648..9376
                     /gene="recO"
                     /locus_tag="ACHURS_003428"
     CDS             8648..9376
                     /gene="recO"
                     /locus_tag="ACHURS_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFR1239077.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            9388..10119
                     /gene="pdxJ"
                     /locus_tag="ACHURS_003429"
     CDS             9388..10119
                     /gene="pdxJ"
                     /locus_tag="ACHURS_003429"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFR1239078.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLTDAGIQISLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            10119..10499
                     /gene="acpS"
                     /locus_tag="ACHURS_003430"
     CDS             10119..10499
                     /gene="acpS"
                     /locus_tag="ACHURS_003430"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFR1239079.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            10721..10801
                     /gene="shoB"
                     /locus_tag="ACHURS_003431"
     CDS             10721..10801
                     /gene="shoB"
                     /locus_tag="ACHURS_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFR1239080.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
BASE COUNT         2715 a         2661 c         2878 g         2558 t
ORIGIN      
        1 gcccgcgcgc cacgccatag caatgccatc gccagaagaa atatccggat tggtggtgta
       61 ctgatacacc ttcgacgcac cgccggttgc cagtaccact gcttttgcgt ggcaggtttc
      121 caccgtttct ttatttcggt tccataccca cgcgcctaca acccgtcgcg tgcccggcag
      181 gccaattttg tcagagacaa tcagatcaac tgcgttgctg cgctccagca cacgaatatt
      241 cggatggttc tgcgccttgc tcaccagcgt ggattgtact tctctaccgg tggcgtcggc
      301 ggcatgaaga atacgacggt gactatgtcc accttcacgg gtcagatggt agctttcttc
      361 gccattcggt tgaacgtggg tatcaaacaa aaccccctgg tcaattaacc attgcacaca
      421 ggaacgagca ttgctggcga caaattcaac tgcatggcga tcgcaaatac cagccccggc
      481 aatcaatgtg tcttccacat gcgagtcaat gctgtcagtt tcatcaaaca cggcggcaat
      541 accgccctgg gcataaaatg ttgaaccttc cgttaccggg cctttactta gaacgatgac
      601 ctgatgctgg tcagccaggc gtagcgccag tgaaagtccg gctgcgccgc taccaataat
      661 caacacgtca catgaatgtt cagggagagt attcattttc tttgtttaat ttactaaaca
      721 ttgtttggtc agcatagcat catgttgtgc gaataaacac ctgctatttt aatatttgtt
      781 acagtggcta aacacgttga cgcagggcgg cgagaaaaag agaagttact ggctggtgga
      841 ggattaggtg gtgaaataaa aaggccgttg ggttactctt caggcagtta aatgggcatt
      901 tctacacaga taatgcgatg ttcagattct gtagacttat aatgatagat aatgatccgt
      961 ctacagcatg acaaacaaaa acagatgcgt tacggaactt tacaaaaacg agacactcta
     1021 accctttgct tgctcaaatt gcagctaatg gagtggcgtt tcgatagcgc gtggaaattt
     1081 ggtttgggga gactttacct cggatgagcg agcagttaac ggaccaggtc ctggttgaac
     1141 gggtccagaa gggagatcag aaagccttta acttactggt agtgcgctat cagcataaag
     1201 tggcgagtct ggtttcccgc tatgtgccgt cgggtgatgt tcccgatgtg gtacaagaag
     1261 cttttattaa agcctatcgt gcgctggatt cgttccgggg agatagcgct ttttatacat
     1321 ggctgtatcg gattgctgta aatacagcga aaaattacct ggttgctcag gggcgtcgtc
     1381 caccttccag tgatgtggat gccattgaag ctgaaaactt cgaaagtggc ggcgcgttga
     1441 aagaaatttc gaaccctgag aacttaatgt tgtcagaaga actgagacag atagttttcc
     1501 gaactattga gtccctcccg gaagatttgc gcatggcaat aaccttgcgg gagctggatg
     1561 gcctgagcta tgaagagata gccgctatca tggattgtcc ggtaggtacg gtgcgttcac
     1621 gtatcttccg agcgagggaa gctattgata acaaagttca accgcttatc aggcgttgac
     1681 gatagcggga tactggataa gggtattagg catgcagaaa gaacaacttt ccgctttaat
     1741 ggatggcgaa acgctggata gtgagctgct taacgaactg gctcataacc cagaaatgca
     1801 gaaaacctgg gaaagctatc acttaatccg tgattcaatg cggggtgata ctcccgaggt
     1861 gctccatttc gatatctctt cacgcgtgat ggccgccatt gaagaagagc cagtacgtca
     1921 accggcgaca ttgatcccgg aagcccagcc tgcgccgcat caatggcaca aaatgccatt
     1981 ctggcagaaa gtacgtccgt gggcggcaca gcttacccaa atgggcgtag cagcatgcgt
     2041 atcgcttgca gttatcgttg gcgtccagca ctataatgga caatctgaaa cgtcccagca
     2101 gcccgaaacg ccggtattta atacactgcc gatgatgggt aaagccagcc cggtaagcct
     2161 gggagtacct tctgaagcga ccgcaaacaa tggtcaacag cagcaggtac aggagcagcg
     2221 tcgtcgcatt aatgcaatgt tgcaggatta cgaactgcaa cgccgactcc actctgaaca
     2281 gcttcagttt gagcaggcgc aaacccagca agccgctgta caggtgccag gacttcaaac
     2341 tttaggaacg caatcgcagt aatgaagcaa ctttggtttg ccatgtcatt agtgacaggt
     2401 agcctgttat tctctgctaa cgcctcggcc actcccgcgt ccggggcgtt attacagcag
     2461 atgaacctgg ccagtcagtc actgaattac gagctgtcat tcatcagcat caataaacag
     2521 ggtgttgagt ctctgcgtta tcgacatgca cgcctcgata accgtcctct tgcacaattg
     2581 ttgcaaatgg atggcccgcg ccgggaagtg gtacagcgcg gcaatgaaat cagctatttt
     2641 gaaccgggac ttgaaccgtt cacgcttaat ggcgattaca ttgttgattc tctgccatcg
     2701 cttatctata ccgatttcaa acgcctttct ccttactacg actttatctc cgtcgggcgc
     2761 acgcgtattg ctgatcgtct ttgcgaagtc attcgcgtgg ttgcccgaga tggtacacgc
     2821 tacagctaca tcgtgtggat ggacactgaa tcgaaattac cgatgagggt tgatctcctt
     2881 gatcgcgatg gtgaaacgct ggaacaattt cgcgtgattt cttttaacgt caatcaggat
     2941 atcagcagca gtatgcagac gctggcgaag gcaaatttgc cgccgttgct ttctgttcct
     3001 gtaggtgaaa aagctaaatt cagctggacg ccaacctggt tgccacaggg ttttagcgaa
     3061 gtttccagta gtcgacgtcc gctaccgacg atggacaaca tgcctatcga atcacgtctc
     3121 tattccgacg gattattcag cttctcggta aacgttaacc gcgctacgcc atcgagcacc
     3181 gatcagatgt tgcgcaccgg acgcagaacc gtcagtacaa gcgtacgtga taacgccgaa
     3241 atcaccattg tcggtgaact gccgccgcaa acggcgaaac gcattgccga gaatattaag
     3301 ttcggggcag cgcaatgatc aaagagtggg ctaccgtcgt ctcctggcaa aacgggcagg
     3361 cgctggtcag ttgtgatgtt aaagcctcat gcagcagctg cgcttcacgc gccggttgcg
     3421 gtagccgcgt gttgaataaa cttggcccgc aaaccacgca taccattgtc gtaccctgtg
     3481 atgaaccgtt agtgccgggg caaaaagtgg aattggggat cgccgaaggc agcctgctta
     3541 gctccgcatt actggtttat atgtcgccgc tggtgggatt attccttatc gcttcgctat
     3601 ttcagctact ctttgcttct gacgttgcag cattatgtgg tgcgatcctt ggcggtattg
     3661 gcgggtttct gattgcgcgc ggttactcgc gtaagtttgc tgcccgggca gaatggcaac
     3721 cgatcatatt aagcgtggca ttgccgcctg accttgtgcg atttgaaaca tcttcggaag
     3781 acgcgagcca gtgatttcac tggctttgtt gcattttgca taacaaatgc gaggacgttt
     3841 ccagatacca caatccatac ggtatgaaca tttcctcgcc tcaatgttgt agtgtagaat
     3901 gcggcgtttc tattaataca gacgttaagc tcagaacagc gacttctaaa agcctggtta
     3961 accgggcatt aaggcacaat aatcatactt tatgaagaat atacgtaact tttcgatcat
     4021 agctcacatt gaccacggta aatcgacgct gtctgaccgt attatccaga tctgcggtgg
     4081 cctgtctgac cgtgaaatgg aggcgcaggt tctcgattcc atggatcttg agcgtgagcg
     4141 tggcattacc atcaaagcgc aaagcgtgac gctggactac aaagcgtctg acggcgaaac
     4201 ctatcagctt aactttatcg acaccccggg ccacgtagac ttctcctatg aagtttcccg
     4261 ttcgctggct gcctgtgaag gtgcattgct ggtggtcgac gccgggcagg gcgtggaagc
     4321 gcaaaccctg gcaaactgct acaccgccat ggaaatggat ctcgaagtgg tgccagtact
     4381 gaacaagatt gacctgccgg cagccgatcc tgaacgcgtg gcggaagaaa ttgaagatat
     4441 cgtcggcatc gacgccactg acgcggtgcg ctgttcagcg aaaaccggcg ttggcgtgca
     4501 ggacgttctc gaacgtctgg tgcgcgacat tccgccgccg gaaggcgatc cggaaggccc
     4561 gttgcaggca ctaattatcg actcatggtt cgacaactac ctgggcgttg tttcacttat
     4621 ccgtattaaa aacggcaccc tgcgtaaggg cgacaaagtg aaagtcatga gtaccgggca
     4681 gacctataac gccgaccgtc tgggcatctt cacgccgaaa caggttgacc gcactgaact
     4741 gaaatgtggc gaagtaggct ggctggtatg tgcgattaaa gatatccacg gcgctccagt
     4801 cggcgatacc ttaacgctgg cgcgtaatcc ggcagaaaag gcgctgcctg gctttaagaa
     4861 agtcaaaccg caggtatacg ccggtctgtt cccggtaagt tccgacgact atgaagcctt
     4921 ccgtgacgcg ctgggtaaac tcagcctgaa tgatgcctca ctgttctatg agccggaaag
     4981 ctccagcgcg ctgggctttg gtttccgctg cggcttcctt ggcctgctgc acatggagat
     5041 catccaggaa cgtctggaac gtgaatacga tctggatctg atcaccactg cgccgaccgt
     5101 agtgtatgaa gttgaaacca cgtcaaggga agttatctac gtcgacagcc catccaagct
     5161 gcctgcggta aataacattt acgaactgcg cgagccgatt gcagagtgtc acatgctgct
     5221 gccgcaggca tatctcggca acgttattac gctgtgcgta gaaaaacgcg gcgtgcagac
     5281 caatatggtt taccacggta atcaggtggc gctgacgtac gagatcccga tggcggaagt
     5341 ggtgctcgac ttcttcgatc gcctgaaatc tacctcgcgt ggttatgcgt ctctggatta
     5401 caacttcaaa cgcttccagg cgtccgacat ggtacgtgta gacgtattaa tcaacggtga
     5461 acgtgttgat gcgttggcgc tgatcaccca ccgtgataat tcgcaaaacc gcggtcgcga
     5521 gttggtggag aagatgaaag atctgatccc acgccagcag tttgatatcg ccattcaggc
     5581 agcgattggt acgcacatta ttgcgcgatc caccgtgaaa cagctgcgta aaaacgtact
     5641 ggctaaatgt tatggcggcg atatcagccg taagaaaaag ctgctgcaga agcagaaaga
     5701 aggtaagaaa cgcatgaagc agatcggtaa cgtcgagctg ccgcaggaag cgttcctcgc
     5761 cattctgcac gtcggcaaag acaacaaata acccttagga gttggcatgg cgaatatgtt
     5821 tgccctgatt ctggtgattg ccacactggt gacgggcatt ttatggtgcg tggataaatt
     5881 ctttttcgca cctaaacggc gggaacgtca ggcagcggcg caggcggctg ccggtgactc
     5941 actggataaa gcaacgttga aaaaggttgc accgaagcct ggctggctgg aaaccggagc
     6001 ttctgttttt ccggtgctgg ctatcgtatt gattgtacgt tcgtttattt atgaaccgtt
     6061 ccagatcccg tcaggttcga tgatgccgac tctgttaatc ggtgatttta ttctggtaga
     6121 gaagtttgct tatggcatta aagatcctat ctaccagaaa acgctgatcg aaaccggtca
     6181 tccgaaacgc ggcgatatcg tggtctttaa atatccggaa gatccaaagc ttgattacat
     6241 caagcgcgcg gtgggtttac cgggcgataa agtcacttac gatccggtct caaaagagct
     6301 gacgattcaa ccgggatgca gttccggcca ggcgtgtgaa aacgcgctgc cggtcaccta
     6361 ctcaaacgtg gaaccgagcg atttcgttca gaccttctca cgccgtaatg gtggggaagc
     6421 gaccagcgga ttctttgaag tgccgaaaaa cgaaaccaaa gaaaatggaa ttcgtctttc
     6481 cgagcgtaaa gagacactgg gtgatgtgac gcaccgaatt ctgacagtgc cgattgcgca
     6541 ggaccaggtg gggatgtatt accagcagcc agggcaacaa ctggcaacct ggattgttcc
     6601 gccgggacaa tacttcatga tgggcgacaa ccgcgacaac agcgcggaca gccgttactg
     6661 gggctttgtg cctgaagcga atctggtcgg tcgggcaacg gctatctgga tgagtttcga
     6721 taagcaagaa ggcgaatggc cgacaggtgt gcgcttaagt cgcattggcg gcatccatta
     6781 atagcccatc ttcgttcacg ttgtcgccgt tatggcgaca acgtgaatta tttatgagat
     6841 aaatctcccg tggctaacga catcccccgt cgttgtgtat agaatattcc cccgaagttt
     6901 aaggttggca cctccaggtt gccacggcac acgaaacagc gttggtcccc atataccggt
     6961 aaactgaaac tgcagcgaag cagttagcag agccatgtat atcaggtctg tttcgtgtgc
     7021 tgaattgttg acgcatttat ttattggtat cgcatgaacc ccatcgtaat taatcggctt
     7081 caacggaagc tgggctacac ttttaatcat caggaactgt tgcagcaggc attaactcat
     7141 cgtagtgcca gcagcaaaca taacgagcgt ttagaatttt taggcgactc tattctgagc
     7201 tacgttatcg ccaatgcgct ttatcaccgt ttccctcgtg tggatgaagg cgatatgagc
     7261 cggatgcgcg ccacgctggt ccgtggcaat acgctggcgg aactggcgcg cgaatttgag
     7321 ttaggcgagt gcttacgttt agggccaggt gaacttaaaa gcggtggatt tcgtcgtgag
     7381 tcaattctcg ccgataccgt cgaagcatta attggtggcg tattcctcga cagtgatatt
     7441 caaaccgtcg agaaattaat cctcaactgg tatcaaactc gtttggacga aattagccca
     7501 ggcgataaac aaaaagatcc gaaaacgcgc ttgcaagaat atttgcaggg tcgccatctg
     7561 ccgctgccga cttatctggt agtccaggta cgtggcgaag cgcacgatca ggaatttact
     7621 atccactgcc aggtcagcgg cctgagtgaa ccggtggttg gcacaggttc aagccgtcgt
     7681 aaggctgagc aggctgccgc cgaacaggcg ttgaaaaaac tggagctgga atgagcatcg
     7741 ataaaagtta ctgcggattt attgccatcg tcggacgtcc gaacgttggc aaatccacat
     7801 tgttgaacaa actgctgggg cagaagatct ccatcacctc ccgcaaagcg caaacaactc
     7861 gtcaccgcat tgtggggatc catactgaag gcgcgtatca ggcgatctac gttgataccc
     7921 ctggcctgca tatggaagaa aaacgcgcta ttaaccgcct gatgaacaaa gcggcgagca
     7981 gctctattgg cgacgttgag ctggtgattt ttgtcgttga aggcacccgc tggacgccgg
     8041 acgacgaaat ggtgctcaac aaactgcgcg atggcaaagc gccggtgatc ctcgcggtga
     8101 acaaagtgga taacgtgcag gaaaaagccg atctgctgcc gcacttacag ttcctggcaa
     8161 gtcagatgaa cttcctcgat atcgtgccga tctctgctga aaccgggctg aatgtcgaca
     8221 ctatcgcagc aatcgtgcgt aagcatctgc ctgaagcgac ccatcacttc ccggaagatt
     8281 acatcaccga tcgctcacag cgttttatgg cgtctgaaat catccgcgaa aaactgatgc
     8341 gtttcctcgg cgctgaactg ccgtactccg tgaccgtgga gatcgaacgt ttcgtctcta
     8401 acgaacgcgg tggttatgac atcaatggtt tgattctcgt tgagcgtgaa gggcagaaga
     8461 agatggttat tggcaacaaa ggggccaaga tcaaaaccat cgggattgaa gcgcgtaaag
     8521 acatgcagga aatgttcgaa gcgcctgtcc accttgaact gtgggtaaaa gtgaaatccg
     8581 gttgggccga cgacgaacgc gcactgcgca gtctcggtta cgttgacgat ctttaagagt
     8641 cactccgatg gaaggctggc agcgcgcatt tgtcctgcat agtcgcccgt ggagcgaaac
     8701 cagcctgatg ctggacgtct tcacggagga atcgggtcgc gtgcgtctgg ttgccaaagg
     8761 cgcacgctct aaacgctcta ctctgaaagg tgcattacag cctttcaccc ctctcttgct
     8821 acgtttcggc gggcgtggcg aagtcaaaac gctgcgcagt gctgaagctg tctcgctggc
     8881 gctgccatta agcggtatca cgctttacag cggtctgtac atcaacgaac ttctctcccg
     8941 cgtactggaa tacgagacgc gcttctctga actctttttc gattacttgc actgcattca
     9001 gtctcttgca ggtgccactg gtacgccaga acccgcgctg cgccgctttg aactggcact
     9061 tctcgggcat ctgggctatg gcgtcaattt tacccattgc gcgggtagcg gcgagccggt
     9121 agatgacacc atgacgtatc gttatcgcga agaaaaaggg tttatcgcaa gcgtcgttat
     9181 cgacaataaa acgttcaccg gaaggcagtt aaaagcgtta aacgcgcggg aatttcctga
     9241 cgcagacaca ctacgcgccg cgaaacgctt tacccgcatg gcgcttaagc cgtatcttgg
     9301 cggtaaacca ttaaagagca gggaactgtt ccggcagttt atgcctaagc gaacggtgaa
     9361 aacacattat gaatgatgag gattgtcatg gctgaattac tgttaggcgt caacattgac
     9421 catattgcaa cgctgcgtaa cgcacgcggt actgcttacc cggatccggt gcaggcggcg
     9481 tttattgccg agcaggcggg agcggacggc attaccgtgc atttacgtga agatcgccgc
     9541 cacattactg accgcgatgt gcgcatcctg cggcagacgc tggatacccg aatgaatctg
     9601 gagatggcag tgaccgaaga gatgctggcg atcgccgttg agacgaagcc acatttttgc
     9661 tgcctggtac cggaaaagcg tcaggaagta acaaccgaag gcggcctgga cgttgcaggg
     9721 cagcgtgaaa aaatgcgcga tgcctgcaaa cgtctgacag atgccgggat tcagatttct
     9781 ctctttatcg acgccgatga agagcagatc aaagctgcgg cagaggttgg cgcaccgttt
     9841 atcgagatcc acaccggttg ctatgctgat gccaaaactg acgccgaaca ggcgcaagag
     9901 ctggtgcgta tcgccaaagc cgcgaccttt gccgctagtc tcggtctgaa agttaacgcc
     9961 ggacacggtc tgacctatca caacgtgaaa gccattgccg ccatccctga gatgcatgaa
    10021 ctgaatatcg gtcatgccat tattggccgt gcagtgatga ccggactgaa agatgcggtg
    10081 gcagaaatga agcgtctgat gctggaagcg cgtggctaat ggcaatatta ggtttaggca
    10141 cggatatcgt agagatcgct cgcatagaag cagtgatcgc ccgatccggt gagcgcctgg
    10201 cacgccgcgt attaagcgat aacgaatggg caatctggaa aacgcaccac cagccggtgc
    10261 gttttctggc gaagcgtttt gctgtgaaag aagccgcagc aaaagcgttt ggcaccggga
    10321 tccgcaatgg tctggcgttt aatcaatttg aagtattcaa tgatgagctc ggcaaaccac
    10381 ggctacggct atggggtgag gcattaaaac tggcggaaaa gctcggcgtt gtaaatatgc
    10441 atgtaacgct ggcagatgaa cgccactatg cttgtgccac ggtaattatt gaaagttaaa
    10501 ataataccgt tctctttaaa aagtaaaacc cccgaactgg taaggaacgg gggttttact
    10561 ttttaacttt aattttgtgc agcatgcacc ctcaacaaca gcgattatgg atcagtgtac
    10621 ttatctttta caagcaactt gcaatcttta gcataaaaac tcaagccttt acgaagaaag
    10681 caatactgaa cggctgcatt ccttgtttaa ggaaaaacga atgactgatt gccgatacct
    10741 gattaaacgg gtcatcaaaa tcatcattgc tgttttacag ctgatccttc tgttcttata
    10801 acacaaggaa ac
//
LOCUS       DAZPDU010000150        10687 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZPDU010000150 DAZPDU010000000
VERSION     DAZPDU010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10687
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.152"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..784)
                     /locus_tag="ACHURS_003432"
     CDS             complement(<1..784)
                     /locus_tag="ACHURS_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFR1239081.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTK"
     gene            957..1835
                     /gene="yihU"
                     /locus_tag="ACHURS_003433"
     CDS             957..1835
                     /gene="yihU"
                     /locus_tag="ACHURS_003433"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFR1239082.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTPAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            1860..2747
                     /locus_tag="ACHURS_003434"
     CDS             1860..2747
                     /locus_tag="ACHURS_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFR1239083.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASVVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESTIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            2780..3790
                     /locus_tag="ACHURS_003435"
     CDS             2780..3790
                     /locus_tag="ACHURS_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFR1239084.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQELKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKMLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDVVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            3860..5293
                     /locus_tag="ACHURS_003436"
     CDS             3860..5293
                     /locus_tag="ACHURS_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1239085.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASGTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLINWEPLGANVDAEDAEVLAELKK"
     gene            5392..6741
                     /locus_tag="ACHURS_003437"
     CDS             5392..6741
                     /locus_tag="ACHURS_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1239086.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIAAFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLVLMVLRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            6731..7618
                     /locus_tag="ACHURS_003438"
     CDS             6731..7618
                     /locus_tag="ACHURS_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFR1239087.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRISPQAGPWD
                     DCFGFYNGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            7759..8358
                     /gene="yihX"
                     /locus_tag="ACHURS_003439"
     CDS             7759..8358
                     /gene="yihX"
                     /locus_tag="ACHURS_003439"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFR1239088.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            8352..9224
                     /locus_tag="ACHURS_003440"
     CDS             8352..9224
                     /locus_tag="ACHURS_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFR1239089.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLFSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            9221..9658
                     /gene="dtd"
                     /locus_tag="ACHURS_003441"
     CDS             9221..9658
                     /gene="dtd"
                     /locus_tag="ACHURS_003441"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFR1239090.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            9703..10644
                     /gene="fabY"
                     /locus_tag="ACHURS_003442"
                     /gene_synonym="yiiD"
     CDS             9703..10644
                     /gene="fabY"
                     /locus_tag="ACHURS_003442"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFR1239091.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFINQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
BASE COUNT         2462 a         2531 c         2855 g         2839 t
ORIGIN      
        1 cttttgttgg cattttttct gccaacgcta ccgcgagtgc gccgtgaaaa acatcgcctg
       61 ctccagtggt atcaaccaca ttgacagaaa atgcctcctg ttgacacaaa tgaccatctt
      121 caatccacaa cgatccttca gaacccagcg taacatacac tttacctgct gtttgtgtcg
      181 ttgcctggaa caaaccttct tcaggtgact gcaggccagt catgcgtttc agccctggtg
      241 ttgagaacac ggcgtgatcg gcaagcgcga caagtggaga aatatcctgc ggtgtcatat
      301 cggcgtctaa aagcgttgtc acacccgcaa ggcgcgctag cgagaatgct ttttcagtac
      361 cgctatgcca gcgaacatcg gccagaatca gatcataacg agagaaatcg atcgcttcta
      421 accattcggc atcagtaccg agatcaggac ttggataatt aacgataatc cgttcgccat
      481 gctgatcaac gaggatcgct gattgcgacg aacgggcgtt aggatatcgg cggcaaaatg
      541 cggtattgac gccccaaccc tcaagttctg caagtaaggt atttccacaa ctgtcatcgc
      601 cgacgcgacc gatgaaatca acctccacgc caagctttgc gattgcaaca gcggctgttg
      661 ctgccgggcc gccaccaatc tcaagataat gatttgcctg atatttcccg ccgccctccg
      721 gcagagtggg caaagaataa attctgtcct gaactgtgat acccacgcat gcaattctgg
      781 tcatatttgt cttgtcctgt ttaattttgt cgtctttggt caatttaaat gacgcaaatg
      841 atcaaattag tgatgcgtgt cgtgtttttg aacataaatt acaaatatgc tcaaaaacag
      901 acgaaaacga attttcaatt gtcattattt tgtcaatgtc agacaggaga gtattcatgg
      961 caaccattgc atttctcggg ctggggcaga tgggtagccc aatggctaac aatctattgc
     1021 aaaaagggca ttcattgcag gtatttgatg tcaatgcaca ggccgtcgat gcgctagtta
     1081 cgcagggggc cactgcggca caaaccccag cggaagcagc agcaggcgct gaatttatca
     1141 tcactatgct tcccaatggt gacattgttc gacaggttct gttgggggaa aaaggcgtct
     1201 gtgaggctgt ttcatctgac gcgttattga ttgatatgtc gactattcat cccttacaga
     1261 ccgatgcatt aatacgggag ttacaagaaa aaggcatcag tatgatggac gctcccgtgg
     1321 gcagaacgtc cgtgaatgcg attgatggca cattgctgat tttagcgggt ggcactgatg
     1381 agcagattgc acgtgcacgc ccgattctca tgtgcatggg taatgagctg gtcgaggctg
     1441 gcggcccagg aatggggatc cgcgtgaagc taattaacaa ctatatgagc attgcgctta
     1501 atgcgctgtc ctccgaggcc gctgtgttat gtgagtcact gggattaaat cttgatgtcg
     1561 caattaaggt gatgagtggt acggcagcag ggaaaggaca ttttacgacc acctggcctg
     1621 gcaaagtgct gaaaggcgat ctttcacccg ccttcatggt ggatctggcg ttgaaagatc
     1681 tgcgtattgc agttgacgtc gctcgggaga cgggggcacc gctcaatatg gggattgcag
     1741 cagaaagtta ttacgctgca gcaagtcaag acggcaaagg gcggcaggac tggagtgccc
     1801 tgctaaatca ggttcgccag caggctggtc tttaatttta tctattaaca ggagtttcta
     1861 tgtcatccca cttcacccca tttacgctga aagatatctc ccgcccaggt ggtggatttg
     1921 ccatgttagc tgtcgatcag cgtgaggcca tgcgcttgat gtttgcggca gcagggcagc
     1981 caaaacccat cgctgacagt gtactcaccg attttaaagt tgccgcgacc cgaatccttt
     2041 ctccttatgc ttctgtcgtt ctggcggata aacaattttg tcttgagcaa atagtcgagc
     2101 agggcgctgt tgctaattct tgcggcctga ttgtcgctgc cgatcttttt atcccaggta
     2161 atggcatccc ggttgatagc gttgaaatag atatgtccgt cgacccgcat aaagcccgtg
     2221 aaatgggggc taaggcaatg aaattacttg tactctggcg tgaagatgaa cccgcagagg
     2281 aacgtctggc aatggttgat aaatttgttc gtcgctgccg tagtgccggg ctggtgagca
     2341 ttattgaacc tgtggtgcgt ccacctcgtc gcggttggga ctttgatcgt gaaagcacca
     2401 ttgttgccgc cgccgcagag cttggtggaa cagaggctga tctttacaaa gccgagatgc
     2461 cattaggggg caaaggcgat gaaaaaacct tacttgccgc ctgtcagcaa cttaacgatc
     2521 agatgaagat gccgtgggta attctttctt ctggcgttga tgccgatatt tttggccgtg
     2581 cagtaagtat tgccatgaaa ggtggtgcgt cgggcttcct ggctggacga gcggtttggg
     2641 cgtcggttgt tggtgcacaa gatccacaaa ctatgctgcg cgatgtctct gttcctcgtt
     2701 tacaacggtt ggcagaaatt gtcgatgaag gtatcgcgca acgctaatcc cctatggata
     2761 tcgattctgg agaataacaa tggataaaat tacccaggtt ttattttcag atattgggaa
     2821 agtcaccact caatatgttg aagtaccaca ccaggaactt aaaccgcacg aagtgcggat
     2881 tgcgcctgtg ttctatggga tatgcggttc ggatctgcat gttctgaaag gcggtcatcc
     2941 gtttgccaaa ccacctgtcg tccccggtca tgaaattgca gcgcgcgtta cggaagttgg
     3001 cagcgacgtt aaaaatgtac agccgggcga tcatgttgtg gtcgatccca tcatggcttg
     3061 catggaatgc cgagcctgca aagcaggacg ttttaatctt tgtgaaccac cgcaggttgc
     3121 tggttttcgc gcaccgggct ttgctcgctc acaacacatt gttcctgcgc gtaattgcca
     3181 tgtcgcacca gcctctttac cgctaaaaat gttggccttt gccgaaccgg cggcttgtgc
     3241 ccgtcactgc gttaaccgaa tgccgaaagc ttctctggaa agcgtactgg taattggtgc
     3301 cggaacgata ggcttatcca tcgtgcaggc actgcgcatt atgggggcag gtaagattac
     3361 cgtgattgaa cctgacgctg ccaaacgcgc gctggcgtta aagctgggcg cagcagaagt
     3421 ttgggcacca ggtgagctgg ccgcagatgt gcgatttacg ggggcgattg atgtcgttgc
     3481 agcgcaggcc acgcttaacg atgcatgtac ccgtgtatat gccggaggca ccgtcgtgtg
     3541 catgggcgta ccaagtgggc cgcgtgaaat accattaccg atgatgcaac gtttcgagcg
     3601 tgacttgctc aactctggca tgtacatccc tgaagatttc gatgttgtta tcgaatggct
     3661 ggcggatggg cggtttgata ccagtgaact ggttaccgat ttatttgcca ttgaggatgc
     3721 agcggcggca tttgaacgcg cgcagcaaaa tgactccata aaggtcatgc tgcaatttgc
     3781 gccggaatga ttttctaaat ctctgtctgg cgctgcgtca cgtcagcgcc accctacaac
     3841 aattaaaaag gacactgcga tgagtcaaat caccagtcct gcaacttatt caattagtcg
     3901 gccacaggac gtaattgata ttgttaataa gaactctgca atcaacacca gtatcggcgt
     3961 tatttttatt gctttgggcg gcatattgat tgatgcctac caggcggcga tggtggggtt
     4021 tggtaataaa tacattgccg ctcaattcgg catttcgccg ggtcttgctg caaccgtcaa
     4081 tgcatcagta ttaatcgccg cgttaattgg cggtttatta gcgaaccgag taataaaccg
     4141 ctttgggcaa aagcgggcat ttattattgg catggggctg tgcaccatcg gtgctgcagc
     4201 ggtagctatt gctcccagta tctggtgggt gctggtgtgc cgcgtcatca tgggctttgg
     4261 tttaggcatt gacttccctt tggcaaccaa tgccgtggca gagcttcgtg gttcaacgtc
     4321 gaagaaaacc ggaacgtcgg tcaacctctg gcaaatggcc tggtatgttt cgacaaccgt
     4381 tgtttatttg gtacttttgc cgctgcttct gtcgggtatc gctgaagaac aattgtggcg
     4441 ttacggaata ttcatcggag ctatttttgc agtcatcttc atgattttgc gttacttctt
     4501 tattggtgaa tccgcaatgt gggccgcacg cgtcgggcgt taccaggaag cgtgcgacat
     4561 tctgggaaaa cgttatggtg ttcaggctcg cgttgcggca tcgggtacaa cagaagcgaa
     4621 attctcggaa aaagcagaga ataaatacag tggtggatat ggcatcttat ttaatgatcg
     4681 ttaccgcaaa cgcaccattc ttggctgtgt cgtggcaacc atgcaggcgt ggcaatataa
     4741 cgccgtaggt gtttatcttc ctcttacgtt ggcgggaata ataagtggcg ggcttactgg
     4801 cgcgttgacc ggttctgccg tcgtgaatgc cctttgtggg gtgacagggg ggatgatcgg
     4861 ctcgtttatt ctccaacgac tgggtactcg ccgacagtcg atgtatggat ttgctgttgt
     4921 gaccttagca ttgctgtcgt taggcgcact ggcaacgact aatccctggc tgtctttagg
     4981 gttattggga tcaattattt tcttccattc agcgggtcct ggtgggctgg gcatgaccat
     5041 tgccacactc tcttatcctc ccgctattcg ccctactggg gtcggatttg cccgcgctat
     5101 tatgcgcaca ggggcaattg caggactcat tttctggccg atgctgtggg gtgcgttgaa
     5161 aactgaagcg ttttactggt tggcaatcgt gccattcctg gggttcctga cctgcgtatt
     5221 gattaattgg gaaccactgg gtgcaaatgt tgatgctgag gatgcagagg ttctggctga
     5281 attgaagaaa taaattaatg cgcatcggct taaaccgatg cgcattaatt caaactgccc
     5341 acgtttagtg gttcgtatcc cagatttctc gcctaccagg agaggaaaat tatgtctgaa
     5401 aagttacccg caccgcgtga agggctttcc ggtaaagcca tgagacgtgt cgttatgggc
     5461 agtttcgcag gcgcgttaat ggaatggtat gacttcttta tttttggcac ggcagcagga
     5521 ctggtatttg caccgctgtt ttatccagac agcgatcctt ttataggtct tatagccgcc
     5581 tttgcgacct ttggtgtcgg ttttctgact cgtcctttag gtggcatcgt ctttggtcat
     5641 tttggcgata aaatcgggcg aaaaataaca cttatctgga cgctggctat tgtcggttgc
     5701 tccacatttt tgattggttt tattccgacg tatcaggaaa ttggcatttg ggcaccgctt
     5761 gtattaatgg tgctgcgttt gattcagggc tttggtcttg gtggcgagta cggcggagcg
     5821 gcattgatga ccatagaatc agcgcccgaa tcccgacgcg gatttttagg ttcactcccg
     5881 caaacggctg cgtctgtggg tattatgctg gcaaccggta tttttgctct ctgtaaccat
     5941 tttcttactt cggagcagtt tctctcctgg ggatggcgca ttccgttttg gctttctgcg
     6001 gtgatgttga tcgttgggct gtttatccgc ctgcataccg aagaaacact ggattttcaa
     6061 aagcaaaaaa cgacgaataa taaagaaaag tccgttcctc cgttgattga attattcaaa
     6121 aaacatccac gaaatatttt attggcactg ggtgcgaggc tggcggaaag tgtctcctct
     6181 aatattatta acgcctttgg tattgtctat atttccagcc aattagcatt gtcgcgagat
     6241 attcccctga cgggtatgtt gattgcctcg gcgatcggta ttttcagttg tccattggtt
     6301 ggatggttat cagatcgtat aggtcaaaaa agtctgtatt tgtcgggagc aggattttgt
     6361 gtcctgttcg cgtttccttt cttcttatta ctggatagca aaagtacgct catcatctgg
     6421 tgcagtatga tcctcggcta caacttaggt ccgacgatga tgtttgccgt gcagccaaca
     6481 cttttcaccc gcatgttcgg taccaaagta cgatataccg gcctttcttt tgcttatcaa
     6541 ttttccgcga ttcttggcgg actaagcccc cttatcgcct caagtttgct ggcgctaggg
     6601 ggaggaaaac cgtggtatgt cgctctgttt ttatttgctg tctcagttct ttcctttgtt
     6661 tgcgtctggc tgattgagcc gaccgatgaa caagaaaccg cttcttaccg ctacatcagg
     6721 gaacaatctc atgaaaactg aacgaaaaaa cgagcattac ctggcgctac aacaggcgtt
     6781 tgacgcgcca tggcctgggc ctgtaggaga gttagtgacg ctggaaaaag gcaatattca
     6841 cctgcagatt tatccgcatg atggtgccag gataacctcg ttaaaggcat ttggttctga
     6901 agtattacgc caatggcagc cgcagcgcag agcttttcag tatggctgtt tcccaatggt
     6961 gccgtgggca gggcgactgg ggaatgcgac gctgaatgct ggtgggcaat gttattcttt
     7021 acccgccaat aaaccaccac atgcgttgca tggtatggct tgttactcaa cgtgggaaat
     7081 catcgacaaa acagcagatt ccctgacttt acgtatgccc ctggcttcac cgtggccctg
     7141 gcaaggggaa gtgattcaga cttttttgct ggagaacgat gcgctggttt tgcaactgga
     7201 agtccattca tatgccgata cattcccggc ctccgcaggc tggcatccgt ggtttgcgaa
     7261 aaagctgact ccgcaaaata cagaatcgtt gcaggtgctt tttgatgcgg actggcaaga
     7321 agaagcgggt agtgatgaac tgccaactgg aaatcgtatc tctcctcaag ccggtccctg
     7381 ggatgactgt tttggttttt ataacggggt gaaggttaag ttactgtggc ctggtaaact
     7441 cgcgatgacc atgatctctt ctgcaaacag tctggtggta tttgataaac agcccgatgc
     7501 cacctgtatt aacccgctga ctcaggcacc taacgcaata aatctaacgc cggaattggt
     7561 gacaccagat aggccgctgg tcatcgaaac tcgttggcaa tttacacctg aatcttaacc
     7621 attcatctat tgcgaaaaag ccagtgaaat tactctgctt tttcgcaatc agcatcacaa
     7681 gattaccctt attgccacat agccaaaccc atcctttccc gctacagtta atttcttgtg
     7741 gcgcgaaagg aggcaaaaat gctctatatc tttgatttag gtaatgtgat tgtcgatatc
     7801 gactttaacc gtgtgctggg agcctggagc gatttaacgc gtattccgct ggcatcgctt
     7861 aagaagagtt ttcatatggg ggaggcgttt catcagcatg agcgtgggga aattagcgac
     7921 gaagcgttcg cagaggcgct gtgtcatgag atggctctac cgctaagcta cgagcagttc
     7981 tctcacggct ggcaggcggt gtttgttgcg ctgcgcccgg aagtgatcgc catcatgcat
     8041 aaactgcgtg agcaggggca tcgcgtggtg gtgctttcca ataccaaccg cctgcatacc
     8101 accttctggc cggaagaata cccggaaatt cgtgatgctg ctgaccatat ctatctgtcg
     8161 caagatctgg ggatgcgcaa acctgaagca cgaatttacc agcatgtttt gcaggcggaa
     8221 ggtttttcac ccagcgatac ggtctttttc gacgataacg ccgataatat agaaggagcc
     8281 aatcagctgg gcattaccag cattctggtg aaagataaaa ccaccatccc ggactatttc
     8341 gcgaaggtgt tatgctaaaa accattcagg acaaagccag gcaccgtacc cgtccactat
     8401 gggcctggct aaaactactc tggcaacgta ttgatgagga caacatgaca accctggcag
     8461 gtaaccttgc ctatgtgtct ttgctctcat tagtgccgct ggttgccgtt gtttttgcgc
     8521 ttttcgccgc ttttcccatg ttttccgacg tcagcattca gttacgtcac tttatttttg
     8581 ccaactttct gcctgctact ggcgatgtta ttcagcggta tatcgaacaa tttgttgcca
     8641 attccaacaa gatgaccgcc gttggggcgt gcgggctgat cgtcaccgcg ttattgttga
     8701 tgtactccat cgatagcgcg ttgaatacca tctggcgcag taaacgagcg cgaccaaaaa
     8761 tttactcgtt tgccgtgtac tggatgatct taacgctggg gccgctgctg gcaggggcca
     8821 gtctggcgat cagttcctat ttgttctccc tgcgctgggc gagcgatctc aatactgtca
     8881 tcgataacgt gctgcgtatt tttccgctgc tgttgtcgtg gatctccttc tggttgctgt
     8941 acagcattgt tcctaccatc cgcgtaccta accgcgacgc gattgtcggc gcgtttgtcg
     9001 ctgcactcct gttcgaagca ggaaagaaag gtttcgcgct ttatatcacc atgttcccgt
     9061 catatcagct catttacggc gtgctggcgg tgatccccat tctcttcgtt tgggtctact
     9121 ggacatggtg tatcgtcttg cttggcgcgg aaattactgt cactctcggg gaataccgca
     9181 aactaaaaca agcagctgaa caagaagaag acgacgaacc atgattgcat taattcaacg
     9241 cgtaacccgt gccagcgtca ccgtggaggg agaagtgacg ggcgaaattg gcgcgggact
     9301 tttggtgtta ttgggtgtcg aaaaggatga cgacgaacag aaagcaaacc gtctgtgcga
     9361 gcgtgtgctc ggctaccgca tctttagcga tgccgaaggc aagatgaatc tcaacgtgca
     9421 acaggcgggc ggcagtgtac tggtggtttc ccagtttacc ctcgccgcag ataccgaacg
     9481 ggggatgcgc ccaagtttct ccaaaggcgc atcaccggat cgcgcagagg cgttatatga
     9541 ctatttcgtc gaacgctgcc gccagcaaga gatgaacacg caaacaggac gcttcgctgc
     9601 cgatatgcag gtatcgctgg tcaatgatgg ccccgtgaca ttctggttgc aggtatgagc
     9661 ctgcttccag ggttgtcacg ggaaacaaga gagagtatcg ctatgtatca ccttcgggtt
     9721 ccacaaacag aagaagagtt agagcgttac tatcagtttc gctgggaaat gttgcgtaag
     9781 cccctgcatc aaccaaaagg ttcggaacgc gacgcgtggg atgcgatggc gcatcaccag
     9841 atggtcgtcg acgagcaggg taatctggtg gcggtaggcc gattgtatat taatgccgac
     9901 aatgaagcgt ccattcgctt tatggccgtt cacccggacg tgcaggacaa agggttagga
     9961 acgctgatgg cgatgaccct ggagtcggtg gcgcgtcagg aaggcgttaa gcgcgtgacc
    10021 tgtagcgccc gtgaagacgc ggtggagttt ttcgccaagc tgggattcat taatcagggg
    10081 gagatcacca ccccaactac cacgccgatt cgccattttt tgatgattaa acccgttgcc
    10141 actctggatg acattctgca tcgcggtgac tggtgcgcgc agctgcaaca ggcgtggtac
    10201 gaacatatcc cgttaagcga aaaaatgggc gtgcgcattc agcaatatac cgggcaaaaa
    10261 tttatcacca ccatgccaga aaccggtaat cagaatccgc accatacgtt gtttgccggg
    10321 agtttattct cgctggcgac gctcaccggt tggggactta tctggctgat gctgcgtgaa
    10381 cgccacctcg gcggaacgat tattctggcg gatgcgcata tccgctacag caagccgatt
    10441 agcggtaaac ctcatgcggt agccgacctt ggtgccttaa gcggcgatct cgaccgtctg
    10501 gcgcgcggac gaaaagcacg ggtgcagatg caagttgaaa tctttggcga cgagacgccg
    10561 ggtgcagtgt ttgaaggcac gtatatcgtt ctgcccgcga agccatttgg cccgtatgaa
    10621 gagggcggga acgaagaaga gtagcttatg gagaggggcg gtgctgcctc tctcattcat
    10681 tccggtc
//
LOCUS       DAZPDU010000151        10575 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZPDU010000151 DAZPDU010000000
VERSION     DAZPDU010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10575
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.153"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..656
                     /locus_tag="ACHURS_003443"
     CDS             <1..656
                     /locus_tag="ACHURS_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1239092.1"
                     /translation="VMRTHFPEYEIISSASAEDLTLLQLRRSGLVIADLAGESEDPRS
                     VCEHYYSLISQYREIHWVFMVSRSWYSQAVELLMCPTATLLSDVEPIENLVKTVRSGN
                     THAERISAMLTSPAMNETHDFSYRSVILTLSERKVLRLLGKGWGINQIASLLKKSNKT
                     ISAQKNSAMRRLAIHSNAEMYAWINSAQGARELNLPSVYGDAAEWNTAELRREMSHS"
     gene            614..1291
                     /gene="bglJ"
                     /locus_tag="ACHURS_003444"
     CDS             614..1291
                     /gene="bglJ"
                     /locus_tag="ACHURS_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFR1239093.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1329..2117)
                     /gene="fhuF"
                     /locus_tag="ACHURS_003445"
     CDS             complement(1329..2117)
                     /gene="fhuF"
                     /locus_tag="ACHURS_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFR1239094.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQLYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2258..2494
                     /locus_tag="ACHURS_003446"
     CDS             2258..2494
                     /locus_tag="ACHURS_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFR1239095.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
     gene            complement(2533..2619)
                     /locus_tag="ACHURS_003447"
     tRNA            complement(2533..2619)
                     /locus_tag="ACHURS_003447"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2583..2585),aa:Leu,seq:cag)
     gene            complement(2887..3918)
                     /gene="rsmC"
                     /locus_tag="ACHURS_003448"
     CDS             complement(2887..3918)
                     /gene="rsmC"
                     /locus_tag="ACHURS_003448"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFR1239096.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            4021..4434
                     /gene="holD"
                     /locus_tag="ACHURS_003449"
     CDS             4021..4434
                     /gene="holD"
                     /locus_tag="ACHURS_003449"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFR1239097.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            4403..4849
                     /gene="rimI"
                     /locus_tag="ACHURS_003450"
     CDS             4403..4849
                     /gene="rimI"
                     /locus_tag="ACHURS_003450"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFR1239098.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            4864..5541
                     /gene="yjjG"
                     /locus_tag="ACHURS_003451"
     CDS             4864..5541
                     /gene="yjjG"
                     /locus_tag="ACHURS_003451"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFR1239099.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            5632..7221
                     /gene="prfC"
                     /locus_tag="ACHURS_003452"
     CDS             5632..7221
                     /gene="prfC"
                     /locus_tag="ACHURS_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFR1239100.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYSVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            7614..8219
                     /gene="osmY"
                     /locus_tag="ACHURS_003453"
     CDS             7614..8219
                     /gene="osmY"
                     /locus_tag="ACHURS_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFR1239101.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            8346..8507
                     /locus_tag="ACHURS_003454"
     CDS             8346..8507
                     /locus_tag="ACHURS_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFR1239102.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            8629..9702
                     /locus_tag="ACHURS_003455"
     CDS             8629..9702
                     /locus_tag="ACHURS_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFR1239103.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            9699..10481
                     /locus_tag="ACHURS_003456"
     CDS             9699..10481
                     /locus_tag="ACHURS_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR1239104.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRIHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
BASE COUNT         2574 a         2639 c         2828 g         2534 t
ORIGIN      
        1 aggtcatgag gactcacttc cctgaatatg aaataatatc cagcgcctct gcggaggacc
       61 ttacattatt acaattacgt cgttccggat tagtcattgc tgatttagcc ggtgaaagtg
      121 aagatccacg ttctgtttgt gaacattatt attctttaat ctcacaatat cgggaaattc
      181 actgggtttt catggtgtca cgctcctggt attcccaggc agtagaattg ctcatgtgcc
      241 ctacggcgac gttattgtct gatgttgaac ccattgagaa tctggtcaag accgtacgtt
      301 ccggcaatac gcacgcagag cgtatcagcg ccatgctgac ctctccggca atgaatgaaa
      361 ctcatgattt tagctatcgc tccgtcattc tcactctttc agagcgcaag gtactgcggc
      421 tattaggtaa aggatggggc atcaaccaga tagcttcgtt gcttaagaaa agtaataaaa
      481 ctatcagcgc ccaaaaaaac agtgcgatgc gtcgactggc aattcacagc aacgctgaaa
      541 tgtatgcatg gataaatagc gcgcagggtg caagagaact taacttgcct tctgtttatg
      601 gagatgccgc agaatggaac acagccgaat taagaagaga aatgtcgcac tcatagaaaa
      661 atgcgtcatg agcagtatcg gtattgagag tttattcaga aagtttgcgg gtaaccctta
      721 taagctccat acctatacca gtcaggagtc atttcaggat gccatgtcgc ggatctcatt
      781 tgcggcggtc attttttctt tttctgccat gagaagtgag cgcagagagg gattatcttg
      841 cctgactgaa ctggcgatta agtttccgcg tacccggcgt ttagttattg cggatgatga
      901 tattgaagct cggctgattg gttcattgtc gccatcaccg ctggacggtg tattaagtaa
      961 agcgtcaacg ctggagattt ttcatcagga actctttttg tcattaaatg gtgtacgtca
     1021 ggcgaccgac cgactgaaca atcagtggta cattaaccaa agccggacgc taagcccgac
     1081 ggagagagaa atattgcgct ttatgtcgcg tggctactca atgacacaaa ttgccgagca
     1141 gcttaaacgc aatatcaaaa cgatccgtgc acataaattt aatgtgatgt cgaaactggg
     1201 cgtcagttct gatgcagggt tgttggaggc cgcagatatt ctgttatgta tgcggcattg
     1261 cgaagcaagt aatgtgttgc atccctatta atccgcatga tgccgggttt acttccccgg
     1321 cagtgctttc atttcagcgt acaatcgcca cattgctgca catccggtaa gcgataacgc
     1381 tggcagcagg ttcggcgcac cagcaggcca tcgcgcagca cgacggtacg ccagagtgga
     1441 ttatcttcac cgttcgtgag cgttttctca aaaaagaggg catggcgcag cgattcaacc
     1501 gtcgcctcgc cgagcagttg cttcatctca gtgagatacc agttgatcaa ataaccggta
     1561 ttactccaga taagtttgcc gttgatctct ccggtcgctt ctagtgcctg cacaaccgga
     1621 accagcgcct ggctgattaa cgtttccatt cgctgctgcg gcgaatgtgg tgttgcgttt
     1681 ttatcttcac acacatcgac ccagaaacag gcgacgcgtc cggtttcgtg aaactcagca
     1741 tggaaatgtt ccggcgacac atctaatgcc ttttcctgcg tcagtagcgc cagcattaat
     1801 ggtggcacca tcaggccgat atacaattgt gcccatagtg agatcagcgg tttgttctcg
     1861 cggatcatcg ttggttggtt gcgatagata tgatcggaat agaccgcgag tagagaactt
     1921 agcgcattcg gtgatgacca ttgcgccagc gtcatggcgt taagcggagc gggttcatcc
     1981 aggcggataa actcgagcaa atgttcacga tgtttggcga tcgtcgcacg tacggcttgt
     2041 gcaagcccag catcctgcgg ctggagatgc gttcgccaga tgatatcttc atagagcggt
     2101 gcggaacgat aggccataat cgggatagta atctaaatga taatgattgc taatcctagc
     2161 gataggttta cccgatagca agggatttct ctggcttgca aatgataaaa attatcatat
     2221 gatattggtt atcattatca atgaaagaga tgaaaccatg ttgcaacgta cgctgggcag
     2281 tggctgggga gtgttgctgc cgggattgct gattgcaggg ctgatgtatg cggatttatc
     2341 gccggatcag tggcggattg tcattctgat gggattagta ttgacgccgg tgatgctgta
     2401 tcacaaacag ttacggcatt acgttttgct accatcgtgc ctggcgctta ttgctgccat
     2461 catgctgatg ataatgaatt tgaatcaggg atgaaaaatc aaggaagaaa caagaaagga
     2521 agtaaagata attggtgcga ggggggggac ttgaaccccc acgtccgtaa ggacactaac
     2581 acctgaagct agcgcgtcta ccaattccgc caccttcgca taccatcaat tcttaaaaag
     2641 aattgctacc acggaggcgc attctagtgg ttttcagctt ttcgtcaata gttaattatc
     2701 gccggaggtg taattgctgg aaaaatgtcc atcaggaaac tagcgtgcag gtttggtagg
     2761 catgcggggg cagatgccag atgcgacgct ggcgcgtctt atctggccta cgaagggcta
     2821 acgtgcaggt tttgtaggtc ggataaggcg ttcacgccgc atccgacacg gtattcggcg
     2881 agataattaa cctttcttcg cctggcgggt cataatggcg cgatacacct tgaaacgccc
     2941 ggtttgcgca atcacttcat ggaagccaaa tgtctcatcc agcacgtccg ggtaaggcag
     3001 gaaggcgttc gctacaattc gcagctcgcc gccgctatta agatgacgca ccgcgccgcg
     3061 aatcagcgtt tgcgccgcat ccaggctggt ttgcatccca tcgtggaacg gcgggttgga
     3121 gatgatcata tcaaaacgac ctttcacctc ggaaaagacg ttgctggcaa agacttcacc
     3181 ttcaacacag ttggccgcaa gtgttgcgcg gctggcttct accgctggcg cagagacatc
     3241 gcacaaggtg agacgaattt tcggcgaatg gcgcgcaaag gcaactgaaa gcacccccgc
     3301 gccacagccg acatccagca ctttaccttt cgtgtgcgga gttaacgtcg agagcagcaa
     3361 ctggctaccg acatccagac cgtcgcggct aaacacgcca ggcagcgttt tgaccgtcag
     3421 gccatcgacg ctgtattcgc cccagaattt atcggcatca aataccggct gtttttccag
     3481 acgaccaaaa tagaggccac agcgacgagc gctgtcgact ttattcaacg gcgcataatc
     3541 tgccagcatc tgctcggcgc tgcgcacgcc gctgcggttc tcgccaacga caaaaatatc
     3601 tgtccccact ggcagcagag aaagtaaatt catcaactgg aactgggctt ccggtttgtt
     3661 cttcggccag tagtaaatca gcgtatcgca atctgcgacg tcatccaccg tggcgaccag
     3721 actgaaacgg gcgttatccc ccatctggcg gcttaatacc tgccagtggt ggaattgctg
     3781 ggtatgagca cggctggccg cggtatctaa acgcgcgggc aggtcatcct gtaagtctcc
     3841 ggcaaacaga atacggcttt gttcgaaatc atcactgtga cgcagcaaga cttcacttgc
     3901 cggggtaaat gcagacatgg aatgctcctc aattgatact ggcggcgatt atagccatat
     3961 gttggcgcgg tatcgacgaa tttgctatat ttgcgcccct gacaacagga gcgattcgct
     4021 atgacatccc gacgagactg gcagttacag caactgggca ttacccagtg gtcgctgcgt
     4081 cgccctggcg cgttgcaggg ggagattgcc attgcgatcc cggcacacgt ccgtctggtg
     4141 atggtggcaa acgatcttcc cgccctgacc gatcctttag tgagcgatgt tctgcgcgca
     4201 ttaaccgtca gccccgatca agtgctgcaa ctgacgccag aaaaaatcgc gatgctgccg
     4261 caaggcagtc gctgcaacag ctggcggttg ggtactgacg aaccgctatc actggaaggc
     4321 gctcaggtgg catcaccggc gctcaccgaa ttacgggcaa acccaacggc acgcgccgcg
     4381 ttatggcaac agatttgcac atatgaacac gatttcttcc ctcgaaacga ctgatttacc
     4441 agcggcttac cacattgaac aacgcgccca cgcctttccg tggagtgaaa aaacctttgc
     4501 cagcaaccag ggcgagcgtt atctcaactt tcagttaacg caaaacggca aaatggcggc
     4561 gtttgcgatt acgcaagtgg tgctggatga agctacattg ttcaatattg cggtcgatcc
     4621 tgactatcag cgtcagggat tgggaagggt gctgctggaa catctgatcg acgaactgga
     4681 aaaacgcggc gtggcgacac tatggctgga agtccgtgcc tcaaatgctg ccgccattgc
     4741 cctgtacgaa agtttaggct ttaacgaggc gacgattcgc cgcaattact accccaccac
     4801 ggacggtcgc gaagacgcca tcatcatggc gttgccaatc agtatgtaag acaaggtgga
     4861 ataatgaagt gggactggat tttctttgat gccgatgaaa cgctgtttac ctttgactca
     4921 ttcaccggcc tgcagcggat gtttcttgat tacagcgtca cctttaccgc tgaagatttt
     4981 caggactatc aggccgttaa caagccactg tgggtggatt atcaaaacgg cgcgatcact
     5041 tcattacagc ttcagcacgg gcgttttgag agctgggccg aacggctgaa agttgaagca
     5101 ggcttgctta acgatgcctt tattaatgcg atggcggaaa tctgcacgcc gctgccgggc
     5161 gcggtttctc tgcttaacgc cattcgtggc aacgccaaaa tcggcatcat caccaacggc
     5221 tttagcgcct tgcagcaagt gcgtctggaa cgcacgggcc tgcgtgatta cttcgatttg
     5281 ctggtgattt ccgaagaagt tggcgttgcc aaaccgaata agaaaatttt cgattatgcg
     5341 ctggaacagg cgggcaatcc tgaccgttca cgcgtgctga tggttggcga cactgccgag
     5401 tccgatattc tcggtggcat caacgccggg cttgcgacct gctggctgaa tgcgcacaat
     5461 cgcgagcaac cagaaggcat cgcgcccacc tggaccgttt cttcgttgca cgaactggag
     5521 cagctcctgt gtaaacactg attgcctccc ccccgttgat gggtaaaata gccgcaattt
     5581 ttcgttttca acaagcgcgg cgcgatgccg cttactcaag aagaaagaat tatgacgttg
     5641 tctccttatt tgcaagaggt ggcgaagcgc cgcacttttg ccattatttc tcacccggat
     5701 gccggtaaga ctaccatcac cgagaaggtg ctgctgttcg gacaggccat tcagaccgcc
     5761 ggtacagtaa aaggccgtgg ttccaaccag cacgctaagt cggactggat ggagatggaa
     5821 aagcagcgtg ggatctccat tactacgtct gtgatgcagt ttccgtatca cgattgcctg
     5881 gttaacctgc tcgacacccc ggggcacgaa gacttctcgg aagataccta tcgtaccctg
     5941 acggcggtgg actgctgcct gatggttatc gacgctgcaa aaggtgttga agatcgtacc
     6001 cgtaagctga tggaagttac ccgtctgcgc gacacgccga tcctcacctt tatgaacaaa
     6061 cttgaccgtg atatccgcga cccgatggaa ctgctcgatg aagttgagaa cgagctgaaa
     6121 atcggctgcg caccgatcac ctggccgatt ggctgcggca agctgtttaa aggcgtttac
     6181 cacctttata aagacgaaac ctatctctat cagagcggta aaggccacac gattcaggaa
     6241 gtccgtattg ttaaagggct gaataacccg gatctcgacg ctgcggttgg tgaagatctg
     6301 gcacagcagc tacgtgacga actggaactg gtgaaaggcg cgtctaacga gttcgacaaa
     6361 gaattgttcc ttgcgggcga aatcactccg gtattcttcg gtactgcgct gggtaacttc
     6421 ggcgttgatc atatgctgga tggcctggtg gagtgggctc ctgcgccgat gccgcgtcag
     6481 actgataccc gtaccgtaga ggcgagcgaa gacaaattta ccggcttcgt atttaaaatt
     6541 caggccaaca tggacccgaa acaccgcgac cgcgtggcgt ttatgcgcgt agtatccggt
     6601 aaatatgaaa aaggcatgaa gctgcgccag gtgcgtactg cgaaagatgt ggttatctcc
     6661 gacgcgctga cctttatggc gggcgaccgt tcgcacgttg aagaagcgta tccgggcgat
     6721 atcctcggcc tgcacaacca cggcactatt cagatcggcg acacctttac ccagggtgag
     6781 atgatgaagt tcaccggtat tccgaacttc gcaccagaac tgttccgtcg tatccgcctg
     6841 aaagatccgc tgaagcaaaa acagctgctc aaagggctgg tacagctttc cgaagagggc
     6901 gcggtgcagg tgttccgtcc gatctccaac aacgatttga tcgttggtgc tgttggtgtg
     6961 ctgcagtttg atgtggtggt atcgcgcctg aagagcgaat acagcgttga agcagtatat
     7021 gaatcagtca acgttgccac tgcccgctgg gtagaatgtg cggacgcgaa gaaattcgaa
     7081 gagttcaagc gtaagaacga aagccaactg gcgcttgatg gcggcgataa cctcgcttac
     7141 atcgctacca gcatggtcaa cctgcgcctg gcgcaggaac gttatcccga cgttcagttc
     7201 caccagaccc gcgagcatta attcttgcct ccagggcgcg gtagccgctg cgccctgtca
     7261 atttcccttc cttattagcc gcttacggaa tgttcttaaa acattcactt ttgcttatgt
     7321 tttcgctgat atcccgagcg gtttcaaaat tgtgatctat atttaacaaa gtaatgacat
     7381 ttctgacggc gttaaatacc gttcaatgcg tagatatcag tatctaaagc cgtcgattgt
     7441 cattctaccg atattaataa ctgattcaga ggctgtaatg gtcgttattc atcacccatc
     7501 gcttttgtga tggcgaccat tgacttctgt agagggtgaa gtctctccct attcagcaat
     7561 gcaacctcgt gttgccaggc tcaaattacg agcaaacata caggaataaa tcgatgacta
     7621 tgacaagact gaagatttcg aaaactctgc tggctgtaat gttgacctct gccgtcgcga
     7681 ccggctctgc ctacgcggaa aacaacgcac agactaccaa tgaaagcgca gggcaaaaag
     7741 tcgatagctc tatgaataaa gtcggtaatt tcatggatga cagcgccatc accgcgaaag
     7801 tgaaggcggc gctggtggat catgacaaca tcaagagcac cgatatctct gtcaaaaccg
     7861 atcaaaaagt cgtgaccctg agcggtttcg ttgaaagcca ggctcaggcc gaagaggcag
     7921 tgaaagtggc gaaaggcgtt gaaggggtga cctctgtcag cgacaaactg cacgttcgcg
     7981 acgctaaaga aggctctgtg aagggctacg cgggtgacac cgccaccacc agtgaaatca
     8041 aagccaaact gctggcggac gatatcgtcc cttcccgtca tgtgaaagtt gaaaccaccg
     8101 acggcgtggt tcagctctcc ggtaccgtcg attcccaggc acagagcgac cgtgctgaaa
     8161 gtatcgccaa agcggtagat ggtgtgaaaa gcgttaaaaa tgatctgaaa actaagtaat
     8221 tcgtcgtaat tcgtcctccc gaactttgtc gggaggcgcg atgtgcacca cactaaaaat
     8281 atcgcattga gtgattttca cgctcatatc aagcggtgac attaactatg gtaaaggaga
     8341 cgcttatgtt tcgttggggc atcatatttc tggttatcgc gttaatcgcc gccgcacttg
     8401 ggtttggtgg tctggccggt accgctgcag gcgcagctaa aattgtcttt gtcgtcggga
     8461 ttattctgtt cctggtgagt ttgttcatgg gccgaaaacg accctagatt tcagaccatt
     8521 aagaaaattt tatccaaagc cagtccagcg gactggcttt tgcggtttta gcgaataata
     8581 aattgcgtta ctttgtcatt ctttcacaac aaaaacagga aggcagaggt ggggcagcga
     8641 atacctgtaa cgcttggtaa tattgcgccg ttgtcgctaa ggccgttcca gcctggacga
     8701 atagctctgg tgtgcgaagg cggcggacag cgtggaattt tcacggctgg cgtgctggat
     8761 gagtttatgc gcgcgcagtt taatcctttc gatctttatc tcggcacatc tgccggggcg
     8821 cagaacctct cggcgtttat ctgcaatcag cccggttacg cgcgcaaagt catcatgcgc
     8881 tataccacaa aacgcgaatt tttcgatcca ttgcgctttg tccgtggagg aaatcttatc
     8941 gatctcgact ggctggtgga ggccaccgca agccagatgc cgttgcaaat ggataccgcc
     9001 gcgcggttgt ttgacagcgg caaatcgttt tatatgtgcg cctgtcgtca ggatgactac
     9061 gcgcctaatt actttttacc aaccaaacaa aactggctgg atgtgattcg cgcctccagt
     9121 gcgatacctg gcttttatcg tagcggagtg tcgctggaag gcattaacta cctggatggc
     9181 gggatcagtg atgcgattcc ggttaaagag gcggcaaggc agggcgctaa aacgttggtc
     9241 gtcattcgca ctgtgccgtc acaaatgtac tacacgccgc agtggttcaa acgcatggaa
     9301 cgctggctgg gtgacagtag cctgcagccg ctggtcaatc tggtgcagca tcatgaaacc
     9361 agctatcgtg acattcagca atttattgag aaaccaccgg gcaagctgcg gatattcgaa
     9421 atttatccgc cgaagccatt acatagtatc gcgcttggca gtcggattcc ggcgctgcgt
     9481 gaagactata aacttgggcg tttatgcggt cgttatttcc tcgccacggt tggcaagcta
     9541 ttaactgaaa aagcgccgct tacccgccat ctggtgccag tggtgacgcc ggaatcgatc
     9601 gttattccgc ctgcgccagt cgccaacgat acgctggttg ccgaagtgag cgatgctccg
     9661 caggcgaacg acccgacatt taacaatgag gatctggctt gatttgccgt tttatcgata
     9721 cccactgcca ttttgatttc ccgccgttta gtggcgatga agaggccagc ctgcaacgcg
     9781 cggcacaagc gggcgtaggc aagatcattg ttccggcgac agaggcggaa aattttgccc
     9841 gcgtgcaggc gttagcggaa aaatatcaac cgctgtatgc cgcattgggg ttgcatccgg
     9901 ggatgttgga aaaacatagc gatgtgtctc ttgatcagct acagcaggcg ctggaaaggc
     9961 gtccggcgaa ggtggtggcg gtgggggaga tcggtctgga tctcttcggc gacgatccgc
    10021 aatttgagag gcagcagtgg ttactcgacg aacagctgaa actggcgaaa cgctacgatc
    10081 tgccggtgat ccttcattcc cggcgtatac acgacaaact ggcgatgcat cttaaacgtc
    10141 acgatttacc gtgcactggc gtggttcacg gtttttccgg cagcctgcaa caggccgagc
    10201 ggtttgtaca gctgggctac aaaatcggcg taggcggtac tatcacctat ccacgcgcca
    10261 gtaaaacccg cgatgtcatc gcaaaattac cgctggcatc gttgttgctg gaaaccgacg
    10321 cgccggatat gccgctcaac ggctttcagg ggcagcctaa ccgaccggaa caggctgtgc
    10381 gggtgtttga cgtgctttgc gagttgcgcc cggaaccaga agatgagatt gccgaagtgt
    10441 tgcttaataa tacgtatgcg ttgtttagcg ttagtgggta gcctaccctt cgtaggcagc
    10501 atccggcagt cggcgcataa tgcctgatgc gacgcttaac gcgtcttatc atgcctacag
    10561 gtttgtgccg aaccg
//
LOCUS       DAZPDU010000152        10562 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZPDU010000152 DAZPDU010000000
VERSION     DAZPDU010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10562
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.154"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(71..1099)
                     /gene="malI"
                     /locus_tag="ACHURS_003457"
     CDS             complement(71..1099)
                     /gene="malI"
                     /locus_tag="ACHURS_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFR1239105.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1274..2866
                     /gene="malX"
                     /locus_tag="ACHURS_003458"
     CDS             1274..2866
                     /gene="malX"
                     /locus_tag="ACHURS_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFR1239106.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            2876..4048
                     /gene="malY"
                     /locus_tag="ACHURS_003459"
     CDS             2876..4048
                     /gene="malY"
                     /locus_tag="ACHURS_003459"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFR1239107.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4152..5153
                     /gene="add"
                     /locus_tag="ACHURS_003460"
     CDS             4152..5153
                     /gene="add"
                     /locus_tag="ACHURS_003460"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFR1239108.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5189..6229)
                     /locus_tag="ACHURS_003461"
                     /pseudo
     CDS             complement(5189..6229)
                     /locus_tag="ACHURS_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
     gene            6473..6598
                     /gene="blr"
                     /locus_tag="ACHURS_003462"
     CDS             6473..6598
                     /gene="blr"
                     /locus_tag="ACHURS_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFR1239109.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            6863..7078
                     /gene="ydgT"
                     /locus_tag="ACHURS_003463"
     CDS             6863..7078
                     /gene="ydgT"
                     /locus_tag="ACHURS_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFR1239110.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7164..7604
                     /locus_tag="ACHURS_003464"
     CDS             7164..7604
                     /locus_tag="ACHURS_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFR1239111.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            7681..8262
                     /gene="rsxA"
                     /locus_tag="ACHURS_003465"
     CDS             7681..8262
                     /gene="rsxA"
                     /locus_tag="ACHURS_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFR1239112.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFNGLVKL"
     gene            8262..8840
                     /gene="rsxB"
                     /locus_tag="ACHURS_003466"
     CDS             8262..8840
                     /gene="rsxB"
                     /locus_tag="ACHURS_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFR1239113.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            8833..>10562
                     /gene="rsxC"
                     /locus_tag="ACHURS_003467"
     CDS             8833..>10562
                     /gene="rsxC"
                     /locus_tag="ACHURS_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFR1239114.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQA"
BASE COUNT         2417 a         2731 c         2703 g         2711 t
ORIGIN      
        1 ctgtgtgcca cattttttat cattaacctt gaggaaagga cactaccagg tatagcagat
       61 aaagagagga ttatttcgct gcaatgagcc gggcgggaat aataagattg cgtgaatgcg
      121 tctcttcatg ggtgattttt tgcatcatgc gatccgcaag tgtggtacca agttcccgcg
      181 caggcgtgct ggcccaggta acgggtatat catcaagtgt ggttggtgtc gcatcggtaa
      241 atgccgccag cgaaacttgt tgctcaaagt aacgatcgac tccgctttcc ccgctttgcc
      301 gccctgcttt cagcaaacca aaccatgccc ccatcgcaat agtttcgtta tagcaaacca
      361 cagcactgat ggtcgggtta tgacgtaaaa gcgccgtgat agcttccgcg gcttgcttct
      421 ggctggatgt gcactccagc acccaatcgc tgtgaaacgg caggccaaat tttagtagag
      481 ttgcacaata gcctcccacc cgttctgccc gggttaatga ggaactttgc cctcccagcc
      541 aggcgatccg ctgatgccca ttgcgaatga gatgctccgt caacaactgt gcagcctgca
      601 tgttgtccgg gcgaaccgta tcaacatcat caagataact ggcacgggaa gcgaaaatca
      661 ccgggatagc tttttcttct gccatccgtc gcaggtcatc gctgcttcct gcagccccgg
      721 caattaccac accatcgaca ccctgattca gtaacagtga aaaccgctgt gccagctgct
      781 cgccgtcttt accgccgtga agcaaaaaaa ccatccgtcc ctgcgcttcc agagcttccg
      841 tcaatccggc cgtcaattcg gcgtaaaacg gcgcagataa atcacggacg atcaaaccaa
      901 tgacgccgct ttgcccgccg cgcagcgccg acgcctggcg attgcgcaca aatcccagct
      961 cttcaatggc ggcgttaacg cgttctcctg tggcggtaga gattcgccct ttgccactaa
     1021 gcaccagcga aacggtactt accgacacgc ccgcagccag cgcaacatca tgaatggtta
     1081 tttttttggc ggtagccatg cagatgacct actccctgat tatgtgacag ataaaacgtt
     1141 ttacctttta tttatcttat acccgctatt atcgttgcgt aatgtgattt atgcttcact
     1201 aaaatttgat aaaacgtttt atcttctcgc gcaatttact gaatccagat tgttctctac
     1261 gaggagtcgt tttatgacgg cgaaaacagc accgaaagtc acgctgtggg agttcttcca
     1321 gcagttaggc aaaaccttca tgttacccgt ggcattattg tcgttctgcg gcattatgct
     1381 cggcattggt agttctctta gtagccacga tgtcatcacc ctgatcccgg tcctgggcaa
     1441 ccccgtgttg caggctatct ttacctggat gagtaagatt ggctcgtttg cttttagttt
     1501 cctgccagtc atgttctgta tcgccatccc gctgggtctg gcacgcgaaa acaaaggcgt
     1561 agcggcattc gctggcttcg tcggttatgc ggtaatgaac ctcgcggtaa acttctggtt
     1621 gaccaataaa ggcattctgc caaccacgga cgccgcggtt ctgaaagcca ataacatcca
     1681 gagcattctt gggatccagt cgatcgacac cgggatcctc ggtgcggtga tcgccggtat
     1741 tatcgtctgg atgctgcatg agcgattcca taatatccgc ctgccggatg cgcttgcatt
     1801 cttcggcggt acgcgcttcg taccaatcat ctcctcgctg gtgatgggcc ttgtcggcct
     1861 ggtgattcca ttagtctggc cgattttcgc gatgggtatt agcggcttgg gccatatgat
     1921 aaacagcgcg ggtgatttcg gaccgatgct gtttggtacc ggtgaacgtc tgctgttgcc
     1981 gtttggtctg catcacattc tggtggcatt aattcgcttt accgacgcag gcggcacgca
     2041 ggaagtctgc ggtcaaaccg tcagcggcgc attgaccatc ttccaggcgc aattgagttg
     2101 cccgaccact cacggttttt ctgaaagcgc cacgcgtttc ctttcgcaag gcaaaatgcc
     2161 tgcgtttctc ggcggtctgc caggtgcagc gttagcgatg tatcactgcg cgcgcccgga
     2221 aaatcgccat aaaattaaag ggctgctgat ttctggcctg atcgcctgtg tcgttggcgg
     2281 cacgaccgaa ccgctggaat tcctgttcct gttcgtggcg ccagttctgt atgtcatcca
     2341 cgcgctgtta accggcctcg gcttcactgt catgtctgtg ctcggcgtca ccatcggtaa
     2401 taccgacggc aatatcatcg acttcgtggt gttcggtatt ttgcatggtc tgtcaaccaa
     2461 gtggtacatg gtgccagtgg tggcggcaat ctggtttgtc gtttactacg tcatcttccg
     2521 tttcgctatc acccgcttca atctgaaaac cccggggcgc gatagcgaag tggccagttc
     2581 aatcgaaaaa gccgttgccg gtgcgccggg taaatcaggt tacaacgttc cggcaatcct
     2641 cgaagcctta ggcggtgccg acaatattgt tagcctcgat aactgcatta cccgtctgcg
     2701 tttgtctgtg aaagatatgt cgcttgttaa tgtgcaggca ctgaaggaca atcgggcaat
     2761 tggcgtagta caacttaatc aacataacct gcaggttgtt atcgggccac aagttcagtc
     2821 agtaaaagat gaaatggccg gtctgatgca tactgtccag gcataaggat aagatatgtt
     2881 cgatttttca aaggtcgtgg atcgtcatgg cacatggtgt acacagtggg attatgtcgc
     2941 tgaccgtttc ggcactgctg acctgttacc gttcacgatt tcagacatgg attttgccac
     3001 tgccccctgc attatcgagg cgctgaatca gcgcctgatg cacggcgtat ttggctacag
     3061 ccgctggaaa aacgatgagt ttctcgcggc tattgcccac tggttttcca cccagcatta
     3121 caccgcaatt gatacgcaga cggtggtgta tggcccttct gtcatctata tggtttcaga
     3181 actgattcgt cagtggtctg aaacaggtga aggcgtggtg atccacacgc ccgcctatga
     3241 cgcattttac aaggccattg aaggtaacca gcgcacagta atgcccgttg ctttagagaa
     3301 gcaggctgac ggctggtttt gcgatatggg caagctggaa gccgttttgg cgaaaccaga
     3361 atgtaaaatt atgctcctgt gtagcccaca gaatcctacc gggaaagtgt ggacgtgcga
     3421 tgagctggag atcatggctg acctgtgcga gcgtcatgat gtgcgggtta tttccgatga
     3481 aatccatatg gatatggtct ggggcgagca gccgcatatt ccctggagta atgtggcgcg
     3541 gggagactgg gcgttgctga cgtcgggatc gaaaagtttc aatattcccg cgctgaccgg
     3601 tgcttacggg attatagaaa atagcagcag ccgtgatgcc tatttatcgg cactgaaagg
     3661 acgtgatggg ctttcttccc cttcggtact ggcgttaact gcccatatcg ccgcctatca
     3721 gcaaggtgca ccgtggctgg atgccttacg cgtctatctg aaagataacc tgacgtatat
     3781 ctcagataaa atgaacgccg cgtttcctga actcaactgg cagatcccgc aatccactta
     3841 tctggcctgg cttgatctac gtccgttgaa tattgacgac aacgcgttgc aaaaagcgct
     3901 tatcgagcaa gaaaaagtcg cgatcatgcc ggggtatacc tatggtgaag aaggtcgtgg
     3961 ttttgtccgt ctcaatgccg gctgcccacg ttcgaaactg gaaaaaggtg tggctggatt
     4021 aataaacgcc atccgcgctg ttcgctaacc ccaattgcgc aacgtaaaaa atcgttgcgc
     4081 aatcgtggat ttttaccctg ctttgttttt ataatggtgc gcacttttat atccagaaaa
     4141 agagtgcgac catgattgat accaccctgc cattaactga tatccatcgc caccttgatg
     4201 gcaacattcg tccccagacc attcttgaac ttggccgcca gtataatatc tcgcttcctg
     4261 cacaatccct ggaaacactg attccccacg ttcaggtcat tgccaacgaa cccgatctgg
     4321 tgagctttct gaccaaactt gactggggcg ttaaagttct cgcctctctt gatgcctgcc
     4381 gccgcgtggc atttgaaaac attgaagatg cagcccgtaa cggcctgcac tatgtcgagc
     4441 tgcgtttttc accaggctac atggcaatgg cacatcagct gcctgtagcg ggtgttgtcg
     4501 aagcggtgat cgatggcgta cgtgaaggtt gccgcacctt tggtgtgcag gcgaagctta
     4561 tcggcattat gagccgaacc ttcggcgaag ccgcctgtca gcaagagctg gaggcctttt
     4621 tagcccaccg tgaccagatt accgcacttg atttagctgg tgatgaactt ggattcccgg
     4681 gaagtctgtt cctttctcac ttcaaccgcg cgcgtgatgc gggctggcat attaccgtcc
     4741 atgcaggcga agctgccggg ccggaaagca tctggcaggc gattcgtgaa ctgggtgcgg
     4801 agcgtattgg acatggcgta aaagccattg aagatcgggc gctgatggat tttctcgccg
     4861 agcaacaaat tggtattgaa tcctgtctga cctccaatat tcagaccagc accgtagcag
     4921 agctggctgc acatccgctg aaaacgttcc ttgagcatgg cattcgtgcc agcattaaca
     4981 ctgacgatcc cggcgtacag ggagtggata tcattcacga atataccgtt gccgcgccag
     5041 ctgctgggtt atcccgtgag caaatccgcc aggcacagat taatggtctg gaaatggctt
     5101 tcctcagcgc agaggaaaaa cgcgcactgc gagaaaaagt cgccgcgaag taacaaaaat
     5161 ggatggtgca atttgcacca tccatttttc acgcaaggca caacgtcgcg cgatgtttgg
     5221 cggattcgat gcccagctca atcaactcca ttacctggat tgcctagctt gccggaaccg
     5281 gattttcacc atcgccattt aacgcatcac gaatcgccgc atagtaagcc ggataattcc
     5341 caggcaccgt caacagcgtt tcttcgacac gttcctcacc ttccacgcgg gtaagtacgc
     5401 catcacgcat atcgtagccc cagtcttcct gcggcagacg ctcgccattt ttcagacgtt
     5461 cttcctgtgg atcgaggcca tatttcacat aactgcctcg ggatccatgc acgatatacc
     5521 gtgctgactc agcggctgcc agcatggtac cgtgtaaaat gactcgccgc tgtggatagg
     5581 acaagatggc gtggaaataa tcggtcgact gcgctccggg ccgtaactgt gccaaatcta
     5641 ccgtcatgct gaccggtaaa ccaaatagcg taatggcctg atcaagaaga tgcggtgcta
     5701 aatcgtacca gataccgctg cctgggccgc cctgttcacg ccaacgatcg cgcacctgcg
     5761 gacggaagcg gtcaaaatga gactcaaagt aagcaacttc acccagcacg ccttccgcga
     5821 gcaaaccttt tagcgtcaag aaatcgctat cccaacgacg gttatggaat acagacagca
     5881 cacgccccag gctttttgcc agcgcatcca gctctcgcgc ttgtgacagt gtcacggtaa
     5941 agggtttatc aacgaccaca tgtttacccg cctcaagcgc cgctttggct aacgggaaat
     6001 gggtatcgtt gggtgtagga atgacaatca ggtctatgtt gggatcgtta aacagatgct
     6061 tcggctcaga gacaaccgta accgttggcc agtcggcttt tacttttgtt tcatcactgc
     6121 tggagattac cgccagttcc agcccgggcg tgcccgcaat caggggcgca tggaaggttt
     6181 tgctcgcata accataccca atcaacccaa cacggatgtt gtcgctcatg tcattgcctc
     6241 tcattgaagt atgatggcta tttgacacta tcctttaccc acgctcaaca gtttaataac
     6301 ctgccagcaa taagggatgt tgtttaactt aagtcaaaaa aaatagcgaa ttttccaacg
     6361 acaaaagcta aatatcgcaa aaacctcagt aaaaatcttg ctggagctat tattgctaag
     6421 taacatttac cccctgaagt taatggatca atcaagagag atgtgggctg taatgaatcg
     6481 tcttattgaa ttaacaggtt ggatcgttct tgtcgtttca gtcattcttc ttggcgtggc
     6541 gagtcacatt gacaactatc agccacctga acagagtgct tcggtacaac acaagtaagc
     6601 tctgcacttg tggagcgaca tgctgcccgt ccgggtgcat gttttcactt gtcggatatt
     6661 aaaccaggaa tttattatct tgttcgatgt tgttggtgat tgtcagggat agtaaagcgc
     6721 gttactcttt tcagagcagt ttttcatccc tgaatatttg ccatcagcgc attcttatta
     6781 ttgtaataac acaagcgttc acttttggtg aaattttatc atcactctac ccctttggta
     6841 ttaattattt tatggacctt ttatgactgt tcaggactac ttattaaaat ttcgcaaaat
     6901 cagttcactc gaaagtctgg aaaaactcta cgaccatctt aattacaccc tgacggacga
     6961 tcaggaactg atcaatatgt atcgtgctgc cgatcaccgt cgcgcagagc tggtttctgg
     7021 cgggcgtttg tttgacctcg gccaggtacc gaagtccgtc tggcactatg tccaataaag
     7081 aaagtagcga tcggttataa aagccttata ataacgcccc taaaattaga tttgcctgtt
     7141 tgcgtcgcta ttctggagaa cctatgacca ccacaacgcc acaacgtatt ggaggctggt
     7201 tgcttggccc gttggcttgg cttttagtcg ctctgttgag tacgacgctg gcgttgttgc
     7261 tgtataccgc tgcgttatct tctccgcaaa catttcaaac gctcggcgga caagcactga
     7321 cgacacaaat cttatggggc gtttctttta ttaccgctat cgccatgtgg tattacacac
     7381 tgtggttgac catcgctttc tttaaacgtc ggcgttgtgt tcctaaacac tatattatct
     7441 ggctgcttat ttccgtattg ctggcagtaa aagcattcgc tttttcaccc gtggaagatg
     7501 gcatagccgt tcgccagttg ctgtttactt tgttggcaac cgcattgatc gtcccctatt
     7561 tcaaacgctc gtcgcgggta aaagcgacgt ttgtgaatcc gtaataacct tacagttaac
     7621 ctgttgtcgc ctgctctgga ttaacggata ataggcggct tttttatttc aggccgaaaa
     7681 atgactgact acctgttact gtttgtcgga actgtactgg tcaataactt tgtactggtc
     7741 aagtttctcg gtctctgtcc gtttatgggg gtttccaaaa aactggaaac cgcgatgggc
     7801 atggggctgg caacaacgtt tgtgatgacg ctggcgtcta tttgcgcctg gcttatcgat
     7861 acgtggattt tgatcccact taatctgatt tacctgcgca ccctggcatt tattctggtg
     7921 attgctgtgg tcgtgcagtt caccgagatg gtggtgcgca aaaccagccc ggtgctttac
     7981 cgtctgctgg ggattttttt gccgcttatc accaccaact gtgcagtgct cggcgtggcg
     8041 ttgctgaata tcaatctcgg gcacaatttc ttgcagtcgg cgctgtacgg tttttccgcc
     8101 gctgtcggtt tctcgctggt gatggtgctc ttcgccgcca tccgcgaacg ccttgctgtg
     8161 gctgatgtcc cggcaccttt tcgcggtaat gccattgcgt taattaccgc aggtcttatg
     8221 tctctggcct ttatgggctt taatggtttg gtgaagttgt aatgaatgct atctggattg
     8281 ccgttgccgc cgtgagcctg ctgggcctgg cgtttggcgc cattctgggt tatgcctccc
     8341 gccgttttgc ggtggaagac gatccggtcg ttgagaaaat tgacgaaatc ttaccgcaga
     8401 gccagtgtgg tcagtgcggt tatcccggct gtcgccccta cgcggaagcc atcagctgta
     8461 acggtgaaaa aatcaaccgt tgcgccccag gtggcgaagc tgtgatgcta aaaattgccg
     8521 agttgcttaa tgtcgagccg cagccgctgg atggcgaagc gcaagagcta acgcctgcgc
     8581 ggatggtggc ggttattgat gaaaataact gtattggctg cactaaatgt attcaggctt
     8641 gtccggtaga cgccatcgtt ggcgctaccc gtgccatgca tacggtaatg agtgatctct
     8701 gtacgggctg caatttatgt gttgatccgt gcccgacgca ctgcatctcg ttgcaaccgg
     8761 tcgcagaaac acctgactcc tggaaatggg atctgaacac cattcccgtg cgtatcattc
     8821 ccgtggaaca ccatgcttaa gttattctct gcattcagaa aaaataaaat ctgggatttc
     8881 aacggcggca tccatccacc ggagatgaaa acccagtcca acggtacacc cctgcgccag
     8941 gtaccactgg cgcagcgttt tgttattcca ctgaaacagc atattggcgc tgaaggtgag
     9001 ttgtgcgtta gcgtcggcga taaagtattg cgcggccagc cgcttacccg tggtcgcggc
     9061 aaaatgctgc ctgttcacgc gcccacctcg ggtaccgtta cggctattgc gccccactct
     9121 acggctcatc cttcagcttt agctgaatta agcgtgatta ttgatgccga tggtgaagac
     9181 tgctggatcc cgcgcgacgg ctgggccgat tatcgcactc gcagtcgcga agagttaatc
     9241 gagcgcatac atcagtttgg tgttgccggg ctgggcggtg caggattccc gacaggcgtt
     9301 aaattgcagg gtggcggaga taagattgaa acgttgatta tcaacgcggc tgagtgcgag
     9361 ccgtacatta ccgccgatga ccgtttgatg caggattgcg cggctcaggt cgtagagggt
     9421 attcgcattc ttgcgcatat tctgcagcca cgcgaaattc ttatcggcat tgaagataac
     9481 aaaccgcagg cgatttccat gctgcgcgcg gtgctggcgg actctaacga tatttctctg
     9541 cgggtgattc caaccaaata tccttctggc ggtgctaaac aattaaccta cattctgacc
     9601 gggaagcagg ttccacatgg cgggcgttca tccgatatcg gcgtattaat gcaaaacgtc
     9661 ggcactgctt atgcagtgaa acgtgccgtt attgatggcg agccgattac cgagcgtgtt
     9721 gtaaccctga ctggcgaagc aatcgctcgc ccgggcaacg tctgggcacg gctggggacg
     9781 ccagtgcgtc atttattgaa tgatgccgga ttctgcccct ctgccgatca aatggtgatt
     9841 atgggtggcc cgctaatggg ctttaccttg ccatggctgg atgtcccggt cgtaaagatt
     9901 accaactgtc tgttggctcc ctctgccaat gaacttggcg aaccacagga agaacaaagc
     9961 tgcatccggt gtagcgcctg tgctgacgcc tgccctgcgg atcttttgcc gcaacagttg
    10021 tactggttca gcaaaggtca gcaacacgat aaagctacca cgcataacat tgctgattgc
    10081 attgaatgtg gggcttgcgc gtgggtttgc ccgagcaata ttcccctggt gcaatatttc
    10141 cgtcaggaaa aagctgaaat tgcggctatt cgtcaggaag aaaagcgcgc cgcagaagcc
    10201 aaagcgcgtt tcgaagcgcg ccaggctcgt ctggagcgcg aaaaagcggc tcgccttgaa
    10261 cgacataaga gcgcagccgt tcaacctgca gccaaagata aagatgcgat tgctgccgct
    10321 ctggcgcggg tgaaagagaa acaggcccag gctacacagc ctattgtgat taaagcgggc
    10381 gaacgcccgg ataacagtgc aattattgca gcacgggaag cccgtaaagc gcaagccaga
    10441 gcgaaacagg cagaactgca gcaaactaac gacgcagcaa ccgttgctga tccacgtaaa
    10501 actgccgttg aagcagctat cgcccgcgcc aaagcgcgca agctggaaca gcaacaggct
    10561 aa
//
LOCUS       DAZPDU010000153        10461 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZPDU010000153 DAZPDU010000000
VERSION     DAZPDU010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10461
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.155"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            121..696
                     /gene="ssuE"
                     /locus_tag="ACHURS_003468"
     CDS             121..696
                     /gene="ssuE"
                     /locus_tag="ACHURS_003468"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFR1239115.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSAMKAQELLHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNVHA"
     gene            689..1648
                     /gene="ssuA"
                     /locus_tag="ACHURS_003469"
     CDS             689..1648
                     /gene="ssuA"
                     /locus_tag="ACHURS_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFR1239116.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLIYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VSAEVAALQQQTADLFYENRLVSKKVDIRQRIWQPTHLEGKQL"
     gene            1645..2792
                     /gene="ssuD"
                     /locus_tag="ACHURS_003470"
                     /pseudo
     CDS             1645..2792
                     /gene="ssuD"
                     /locus_tag="ACHURS_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
     gene            2804..3595
                     /gene="ssuC"
                     /locus_tag="ACHURS_003471"
     CDS             2804..3595
                     /gene="ssuC"
                     /locus_tag="ACHURS_003471"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFR1239117.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3592..4359
                     /gene="ssuB"
                     /locus_tag="ACHURS_003472"
     CDS             3592..4359
                     /gene="ssuB"
                     /locus_tag="ACHURS_003472"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFR1239118.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(4566..7178)
                     /gene="pepN"
                     /locus_tag="ACHURS_003473"
     CDS             complement(4566..7178)
                     /gene="pepN"
                     /locus_tag="ACHURS_003473"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFR1239119.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPEHFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7444..8646
                     /gene="pncB"
                     /locus_tag="ACHURS_003474"
     CDS             7444..8646
                     /gene="pncB"
                     /locus_tag="ACHURS_003474"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFR1239120.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8816..10216
                     /gene="asnS"
                     /locus_tag="ACHURS_003475"
     CDS             8816..10216
                     /gene="asnS"
                     /locus_tag="ACHURS_003475"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFR1239121.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2356 a         2691 c         2825 g         2589 t
ORIGIN      
        1 gtttattttc agaaaaagat acacaaaatt tatatttggc attttcttgt ctctccgaca
       61 gactgaccga attgttatca atgttaacaa aaaaagaaca attggttata aggagagagt
      121 atgcgtgtca tcaccctggc gggtagtcct cgctttcctt ctcgctccag ctccttgctg
      181 gaatatgcgc gggaaaaact aaatggcctg gatgtagagg tttatcactg gaatctgcaa
      241 aacttcgccc cggaagatct gctttacgct cgtttcgata gtccggcact caagaccttc
      301 accgaacagc tgcaacaggc tgatggactg atagtcgcta cacctgtgta taaagccgcc
      361 tattccggcg cgctgaaaac cctgctcgac ctgctgccag agcgcgcttt gcaaggcaaa
      421 gtggtgctac cgctggcgac gggcggtacg gtggctcatc tgctggcggt cgattatgcc
      481 cttaagccag ttttaagtgc gatgaaagcc caggagctcc tgcacggcgt gtttgccgat
      541 gactcacaag ttattgatta ccatcacaaa ccccagttca cgccaaatct gcaaacccgt
      601 cttgataccg cgctagaaac tttctggcag gcattgcacc gccgcgatgt tcaggttcct
      661 gaccttctgt ctctgcgagg taatgtccat gcgtaacatc attaaactgg cgctggcggg
      721 attgcttagc gtctctactt ttgcggttgc tgcagaatcc tcgcctgaag cgttacgtat
      781 aggctatcag aaaggcagta ttggtatggt attggcaaaa agccaccagt tactggaaaa
      841 acgctatccg caatcaaaaa tctcctgggt ggagttcccc gcgggtccgc aaatgttgga
      901 agcgttaaac gttggcagta ttgatctcgg cagtaccggg gatattccgc caatctttgc
      961 ccaggctgcc ggggctgatt tgatatatgt gggcgtcgag ccaccgaagc ccaaagccga
     1021 agtgattctg gtggcagaaa acagcccgat caaaaccgta gccgatctta aaggtcacaa
     1081 agttgccttt cagaaaggtt ccagttcaca caacctttta ctgcgtgcac tgcgtcaggc
     1141 cggacttaag tttactgaca tccagcccac ttatctgacg ccagctgatg cccgcgccgc
     1201 gttccagcaa ggtaacgttg acgcctgggc tatctgggat ccctactact ccgctgcgtt
     1261 attacagggc ggcgtgcggg tgctgaaaga cggcaccgat ctcaatcaaa ctggatcgtt
     1321 ttatctggca gctcgcccct atgcagaaaa aaacggcgct tttattcagg gcgtactggc
     1381 aacctttagt gaggccgatg cgttaacccg cagccagcgc gagcaaagca tcgctttact
     1441 ggcaaaaacg atgggcttac cggcaccggt gattgcctcg tatctggatc atcgtcctcc
     1501 caccaccatc aaaccggtga gcgctgaagt tgccgcctta cagcagcaaa cggcagatct
     1561 gttttatgaa aaccgtctgg tgtcgaaaaa agttgatatt cgccagcgca tctggcagcc
     1621 cactcacctg gaaggaaaac aattatgagt ctgaatatgt tctggttttt accgacccat
     1681 ggtgacgggc attatcttgg aacggaagaa ggttcacgcc cggttgatca cggctatctg
     1741 caacaaattg cgcaagcggc ggatcgtctg ggctataccg gtgtgctgat cccaacgggg
     1801 cgatcgtgtg aagatgcgtg gctggtggcg gcgtcgatga tcccggtgac gcagcggctg
     1861 aagtttcttg tcgccctgcg tcccagcgta acctcaccta ccgttgccgc tcgccaggcc
     1921 gccacgcttg accgtctctc aaatggtcgt gcgttgttta atctggtcac aggcagcgat
     1981 ccacaagagt tggcaggcga cggcgtgttc cttgatcata gcgagcgcta cgaagcctcg
     2041 gcggaattta cccaggtctg gcggggcgct tattgctggg ggaaagcgtc gatttcaacg
     2101 gtaaacatat tcatgtgcgg ggagcaaaac tgctcttccc gccgattcaa cagccgtatc
     2161 ctccgcttta ctttggcgga tcgtcagatg tcgcccagga gctggcggca gaacaagttg
     2221 atctctacct cacctggggc gaaccgccag agctggtaaa agagaagatc gaacaagtgc
     2281 gggcgaaagc tgccgcgcat ggacgcaaaa ttcgtttcgg tattcgtctg catgtgattg
     2341 ttcgtgaaac taacgacgaa gcgtggcagg ccgccgagcg gttaatctcg catcttgatg
     2401 atgaaactat cgccaaagca caggccgcat tcgcccggac ggattccgta ggacaacagc
     2461 gaatggcggc gctacataac ggcaagcgcg acaatctgga gatcagcccc aatttatggg
     2521 cgggcgttgg cttagtgcgc ggcggtgccg gaacggcgct ggtgggcgat ggtcctacgg
     2581 tcgccgcgcg aatcaacgaa tacgccgcgc ttggcatcga cagctttgtg ctttcgggct
     2641 acccgcatct ggaagaagcg tatcgggttg gcgagttgct gttcccacat ctggatgtcg
     2701 ccattccgga aattccccag ccgcagccgc tgaatccgca aggcgaagcg gtggcgaatg
     2761 attttatccc ccgtaaagtc gcgcaaagct aaggaacacg atgatggcaa cgccagtgaa
     2821 gaagtggtta ttgcgcgttg ccccctggtt tttaccggtg ggcatcgtgg cggtgtggca
     2881 actggcctcc tcggttggct ggctgtcgac gcgtattttg ccttcaccgg aaggagtggt
     2941 gacggcgttc tggacgctct ccgccagcgg cgaattgtgg cagcatctgg cgatcagttc
     3001 ctggcgggcg ctgattggct tttcaattgg cggatcgctg ggcttgattc tgggactgat
     3061 tagcgggctg tcacgctggg gagaacggct gctggatacc tcaattcaga tgttgcgcaa
     3121 cgtaccgcat ctggcgctga tcccgctggt gattttgtgg tttggcattg atgaatccgc
     3181 aaaaatcttt ctggtggcgc tcggcacgct attccccatt tatatcaaca cctggcatgg
     3241 catccgtaat atcgatcgcg gtctggtgga aatggcgcgt agctatggat tatccggcat
     3301 accgctgttt atccatgtga tcctgcctgg tgccctgccc tcaattatgg tcggcgtgcg
     3361 ttttgcgttg ggcctgatgt ggctgacgct gattgttgcc gaaaccattt ctgccaattc
     3421 aggcattggt tatctggcga tgaatgcgcg ggagtttttg caaacggacg tggtggtagt
     3481 cgctattatt ctttacgccc tgctcggcaa acttgctgac gtcagcgcgc agctgttaga
     3541 gcgcctctgg ttgcgctgga acccggctta tcatttgaag gaggccacgg tatgaatact
     3601 gctcgtctga accagggcac accattgttg ctcaatgcag taagcaaaca ttacgcggaa
     3661 aatatcgtcc tgaaccaact ggatttacat attccggcag gtcagtttgt ggcggtggtg
     3721 ggccgcagcg gtggtggcaa aagtaccctg ctgcgcctgc tggcaggtct ggaaacgcca
     3781 accgcaggcg atgtgttagc gggcaccaca ccgctggctg agattcagga agacacgcga
     3841 atgatgtttc aggatgcgcg tctgctgccg tggaaatcgg tgattgataa cgttgggtta
     3901 ggccttaaag gccagtggcg cgatgccgcg cgtcgggcgt tggctgcagt aggactggag
     3961 aatcgcgccg gggaatggcc tgccgcactt tctggcgggc agaaacagcg tgtggcgctg
     4021 gcaagagcgt tgattcatcg accgggttta ttgttgcttg atgaaccgct cggggcactg
     4081 gacgccttaa cgcgactcga gatgcaggat ttgattgtgt cactttggca gcagcacggc
     4141 tttaccgtac tgttggtgac gcatgatgtg agcgaagcgg tagcaatggc tgaccgggtg
     4201 ttgttaattg aagagggaaa aattggtctg gatttgacag tggatatccc ccgtccgcgt
     4261 cgattagggt cggtgcgatt agcggagctg gaagcggaag tattgcaacg ggtaatgcgg
     4321 cgtgggcatt ctgaacaact gattcggcgt catggttaat atgccggatg tggcgcacaa
     4381 aaccagcatt ttcaacggac ttgtaggcct gataagacac gtcaagcatc gcatcaggca
     4441 gttgggcacg gctgccggat gcggcgtgaa cgccttatcc agcctacaaa accagcaatt
     4501 tcaacggagt tgtaggcctg ataagacgcg tcaagcgtcg catcaggcat tttggctcaa
     4561 aagtatcaag ccagtgcttt ggtgatcttc tcgtacagat cgccagagag attttccagc
     4621 cctttcaact gttccagcgc cgcgcgcatt ttctcctgac gtttggcatc ataacgtttc
     4681 aggcgaatca gcggttcaat cagacgtgaa gctacctgcg ggttacggct gttgaggtcg
     4741 gtaagcattt ccaccaggaa ctggtaaccg ctgccatctt cggcatggaa cgctgccggg
     4801 ttgctgcccg caaacgcacc aatcaacgaa cgaatacggt tcgggttgct catggtaaat
     4861 gagcgatgct gcaacagacc acgcaccgtc tccagcacat tcgccgccgg gctggtggct
     4921 tgcaggataa accatttatc catcaccaga ccgtcctgat gccacttatc gtcgtactcc
     4981 tgcatcagcg cgtcacggca aggcagctgt gcagcaaccg ccgcagaaag cgctgccagc
     5041 gcatcggtca tattgtttgc ttcatggtat tgcttgctca ccagcacgtc agccagatgc
     5101 gtttcaccaa aagcaaggaa gcgcaggcag gcatttcgca gagtgcgttt tgcaatatct
     5161 tcatgctcaa cacggtactc gctctggtaa ttcgcgttgt aaatagccag cagctcatcc
     5221 gccagttcag tcgccagagt acgagtgagt gcttcgcgta cttcggcaat agcaatcggg
     5281 tcgatgatat cgaacagttc agccatttca ttgacagaag gcagcgtcag gatttctgcc
     5341 gccagcgccg gatcaatctt ctcatcgagc agcaccgcgc ggaaagcgtc agccacatgt
     5401 accggcagag acagcggctg cccttgctga tgacgggcga cgttcagctt gatgtaggtt
     5461 gccagcaaac tttgcgccgc atcccaacgg gagaaatcat tacgcgcatg acgcatcagg
     5521 aaggtcagtt gctgatcact ccacttatat tccagtttca ctggcgcaga gaattcgcac
     5581 agcagcgcag gcaccggctg gaagtagaca ttatcaaaaa caaaggtctg ttccgcctgg
     5641 gtgacgttca gcacggaatt caccggatga ccgcctttct gcaacgggat cactttgcct
     5701 tcgttgtcat acaattcgat ggcaaacgga atatgcagcg gctgtttttc tgcctgatcc
     5761 ggcgtggctg gcgtgcgctg gctgatggtc agggtgtact gctcagtttc cggattgtag
     5821 tcgtctttga cggtcacaat cggcgtaccg gactggctgt accaacggcg gaaatgggag
     5881 agatcaacat tcgacgcatc ttccatcgcc tgcacaaagt cgtcacaggt cgctgcgcta
     5941 ccatcatgac gctcgaaata gagttgcatc cctttctgga agttttcttc gcccagcagg
     6001 gtgtggatca tacggatcac ttccgcgccc ttctcgtaaa cggtcagggt gtagaagttg
     6061 ttcatctcaa tgaccatgtc cgggcggatt gggtgcgcca tcgggctggc gtcttccgca
     6121 aactgcaatc cgcgcatggt gcgcacatta ttgatgcggt taactgcgcg ggaaccaagg
     6181 tcagagctga attcctgatc gcggaagacg gttaaacctt ctttcaggct gagctggaac
     6241 cagtcgcggc aggtcactcg gttaccggtc cagttatgga aatattcatg gccgataacg
     6301 cgttcaatat cgaggtaatc tttgtcggtg gcggtgtcgg tgcgggccag cacatatttg
     6361 gagttaaaga tattcagacc cttattctcc attgcgccca tattgaagaa atccaccgcg
     6421 acgatcatat agatgtcgag gtcatactcc aggccgaagc gttcttcatc ccatttcatt
     6481 gagtttttca gcgaggtcat cgcccacggc gcgcgatcaa ggttgccgcg atcgacgtac
     6541 agctccagcg ccacttcgcg accagaacgc gtggtaaagg tgtcgcgcag tacatcaaag
     6601 tcgcctgcca ccagcgcaaa caggtagcac ggtttcggga acgggtcctg ccactgtacc
     6661 caatggcgtc cgttttccag ttcaccttgc gcaacgcggt taccgttgga aagcaggaag
     6721 ggatatttgg ttttatcggc aataattttg gtggtaaaac gcgccagcac gtccgggcgg
     6781 tcgagataat acgtaatatg gcggaaacct tcggcttcac actgggtgca aagcgcatcg
     6841 cctgactgat aaagcccttc cagcgcggta ttcgccgccg ggcttatttc attaacgatc
     6901 ttaagcgtaa aatgctccgg caaattactg atgaccagtg cgccctcttc ttctttccag
     6961 gcggtccacg gctcatcatt aatatgaaca gaaaccagtt tgaggtcttc gccgttgaga
     7021 cgaagtggag catctgatgc accatgacgg acagcctggc tgaccgcggt aacgaccgtc
     7081 ttttgcgcgt cgaggtcaaa ggtcaagtca atatcagtaa tctggtaatc cggcgcacga
     7141 taatcgtgac ggtatttggc ttgtggctgt tgagtcataa ataaccttta gcatctttta
     7201 tagagtctgg tgttcagtct attcctgttg cgtaaatcgc gctatgcaga atcttcatct
     7261 tttcaggtac aaacgccttt attgctacat ttttataaca tacaccgcgt aatgccatcg
     7321 accagaaagg tggcatatgg tgtgatcggg gttcaataaa ttgcgaaaca aggtatactc
     7381 cagcagttcc tgaagatgtt tattgtacta aacgctcctg tacgaggacg ctactgcgca
     7441 cctatgacac aattcgcttc tcctgttctg cactcgttgc tggatacaga tgcttataag
     7501 ttgcatatgc agcaagccgt gtttcatcac tattacgatg tgcatgtcgc ggcggagttt
     7561 cgttgccgag gtgacgatct gctgggtatt tatgccgatg ctattcgtga acagattcag
     7621 gcgatgcagc acctgcgcct gcaggatgat gaatatcagt ggctctctgc cctgcctttc
     7681 tttaaggccg actatcttaa ctggttacgc gagttccgct ttaacccgga acaagtcacc
     7741 gtgtccaacg ataatggcaa gctggatatt cgcttaagcg gtccgtggcg tgaagtcatc
     7801 ctctgggaag ttcctttgct ggcggttatc agtgaaatgg tacatcgcta tcgctcaccg
     7861 caggccgacg ttgcgcaagc cctcgacacg ctggaaagca aattagtcga cttctcggcg
     7921 ttaaccgccg gtcttgatat gtcgcgcttc catctgatgg attttggcac ccgtcgccgt
     7981 ttttctcgcg aagtacaaga aaccatcgtt aagcgtctgc aacaggaatc ctggttcgtg
     8041 ggcaccagca actacgatct ggcgcgtcgg ctttccctca cgccgatggg aacacaggca
     8101 cacgaatggt tccaggcaca tcagcaaatc agcccggatc tagccaacag ccagcgagct
     8161 gcacttgctg cctggctgga agagtatccc gaccaacttg gcattgcatt aaccgactgc
     8221 atcactatgg atgctttcct gcgtgatttc ggtgtcgagt tcgctagtcg gtatcagggc
     8281 ctgcgtcatg actctggcga cccggttgaa tggggtgaaa aagccattgc acattatgaa
     8341 aagctgggaa ttgatccaca gagtaaaacg ctggttttct ctgacaatct ggatttacgc
     8401 aaagcggttg agctataccg ccacttctct tcccgcgtgc aattaagttt tggtattggg
     8461 actcgcctga cctgcgatat cccccaggta aaacccctga atattgtgat taagttggta
     8521 gagtgtaacg gtaaaccggt ggcgaaactt tctgacagcc ctggcaaaac tatctgccat
     8581 gataaagcgt ttgttcgggc gctgcgcaaa gcgttcgacc ttccgcatat taaaaaagcc
     8641 agttaatatc atcagggagc taatcggctc ccttttttta cctttaattc cgaaatcttt
     8701 cgctgcattt gcgaattctg cttgtctgat tgcagatgcc aggtaacata ggtatccccc
     8761 ccatttacgg gtggacaagt gtttattttt tccgactatt aacagagaga atattatgag
     8821 cgttgtgcct gtagccgacg tactccaggg ccgcgtagcc gttgacagcg aagtcaccgt
     8881 acgcggatgg gtacgtaccc gccgagattc aaaagctggc atctccttcc tcgccgttta
     8941 tgacggttcc tgctttgatc ctgtacaggc tgtcatcaat aattctctgc ccaattacaa
     9001 tgaagacgtc ctgcgtctga ccaccggctg ctcggtcatt gtgacgggta aagtcgtggc
     9061 atcgccgggc caggggcaac aatttgaaat tcaggccagc aaggttgaag ttgctggttg
     9121 ggttgaagat ccagacactt acccgatggc ggcaaaacgc cacagcattg agtatctgcg
     9181 tgaagtcgct cacctgcgtc cgcgcacaaa cctgattggt gccgtcgcgc gcgttcgcca
     9241 tacgctggcg caggcgctgc atcgcttctt taacgagcag ggattcttct gggtttcaac
     9301 gccactgatt accgcatctg ataccgaagg tgcaggcgaa atgttccgcg tttctacgct
     9361 ggatctggaa aacctgccgc gtaacgatca gggcaaagtg gatttcgaca aagacttctt
     9421 tggtaaagag tctttcctga ccgtatctgg ccagttgaac ggcgaaacct acgcttgcgc
     9481 attatccaaa atttatacct tcggcccgac tttccgtgct gaaaactcca acaccagccg
     9541 tcacctggcg gaattctgga tgctggagcc ggaagtggcg tttgctaacc tgaacgatat
     9601 cgcgggtctg gctgaagcca tgctgaaata tgtcttcaaa gcggttctcg aagaacgcgc
     9661 agacgacatg aaattcttcg ctgaacgcgt agataaagat gccgtttcac gtctggaacg
     9721 cttcattgag gccgatttcg cgcaggtgga ttacaccgac gcagtgacca ttctcgaaaa
     9781 ctgcggcagg aagtttgaaa acccggttta ctggggcgtc gatctctctt ctgagcatga
     9841 gcgttatctg gcggaagaac actttaaagc accggtagtg gttaaaaact atccgaaaga
     9901 tattaaagcg ttctatatgc gcctcaacga agacggtaaa accgttgcgg ctatggacgt
     9961 tctggctccg ggcatcggtg agatcattgg tggctcccag cgtgaagaac gtctggacgt
    10021 tctggacgag cgtatgctgg aaatgggcct gaataaagaa gattactggt ggtatcgcga
    10081 tctgcgtcgc tacggtactg ttccgcattc aggtttcggt cttggttttg aacgcctgat
    10141 tgcttacgta actggcgtgc aaaacgtgcg tgatgtgatt ccgttcccac gtactccgcg
    10201 caacgccagc ttctgatttt cttcctcaaa gagccagcgc ctgctggctc ttttttatcc
    10261 tttctgtcaa gttatctgtt tgttaagtca agcaatctat ttgcaacccc gccataacga
    10321 tcatcctgtt acggaatatt acattgcaac atttacgcac aaaaaataac ccgcattctt
    10381 attgcggatt agttttttct tagctgatag cacaattttc atactatttt ttggcattct
    10441 ggatgtctga aagaggattt t
//
LOCUS       DAZPDU010000154        10455 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZPDU010000154 DAZPDU010000000
VERSION     DAZPDU010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10455
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.156"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            142..2010
                     /gene="kup"
                     /locus_tag="ACHURS_003476"
     CDS             142..2010
                     /gene="kup"
                     /locus_tag="ACHURS_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFR1239122.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAMHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2177..2596
                     /gene="rbsD"
                     /locus_tag="ACHURS_003477"
     CDS             2177..2596
                     /gene="rbsD"
                     /locus_tag="ACHURS_003477"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFR1239123.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2604..4109
                     /gene="rbsA"
                     /locus_tag="ACHURS_003478"
     CDS             2604..4109
                     /gene="rbsA"
                     /locus_tag="ACHURS_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFR1239124.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVIALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLCFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGAQPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            4114..5079
                     /gene="rbsC"
                     /locus_tag="ACHURS_003479"
     CDS             4114..5079
                     /gene="rbsC"
                     /locus_tag="ACHURS_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFR1239125.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            5104..5990
                     /gene="rbsB"
                     /locus_tag="ACHURS_003480"
                     /pseudo
     CDS             5104..5990
                     /gene="rbsB"
                     /locus_tag="ACHURS_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            6116..7045
                     /gene="rbsK"
                     /locus_tag="ACHURS_003481"
     CDS             6116..7045
                     /gene="rbsK"
                     /locus_tag="ACHURS_003481"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFR1239126.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            7049..8041
                     /gene="rbsR"
                     /locus_tag="ACHURS_003482"
     CDS             7049..8041
                     /gene="rbsR"
                     /locus_tag="ACHURS_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFR1239127.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="ACHURS_003483"
     CDS             complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="ACHURS_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFR1239128.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVSFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLVVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(9457..10149)
                     /locus_tag="ACHURS_003484"
     CDS             complement(9457..10149)
                     /locus_tag="ACHURS_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFR1239129.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLNLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         2557 a         2522 c         2762 g         2614 t
ORIGIN      
        1 aagggggggt atttttcttt gagccaggtt aatgtggccg catttaggag tacgattttg
       61 ccgttaatcg tgcatactgt gcgctttttt gtgggccaag ggactaagca cacatttcat
      121 atttcaacga aagactagtc tatgagcact gataataagc aatcattgcc cgcgattacc
      181 ctcgcggcga ttggagttgt ctacggcgat attggtacca gcccgttata tacacttcgt
      241 gaatgtttgt ccggccagtt tggttttggc gttgaacgcg atgccgtgtt tggcttttta
      301 tcgctgatct tctggctgct aatctttgtg gtttccatta aatatctcac cttcgtgatg
      361 cgggcagata acgccggtga gggggggatc ctgacgttga tgtcgcttgc cgggcgtaat
      421 acgtcggcgc gaaccacatc aatgctggtg attatggggc taatcggcgg cagctttttc
      481 tatggtgaag tcgtcataac acccgctatt tcggtgatgt cagccattga aggtctggaa
      541 atcgtcgccc cgcagctgga tacctggata gttcccctct caattatcgt tctcacttta
      601 ttatttatga ttcaaaagca tggcacggct atggtcggta agctgtttgc gccgatcatg
      661 ctgacctggt ttttgattct ggcaggactg gggttacgta gcattattgc taacccggaa
      721 gtgctgcatg cgctgaatcc gatgtgggcg atgcatttct tccttgaata caaaacggtt
      781 tcttttattg cattaggggc agtggtgttg tcgattacgg gggtcgaggc gctgtatgct
      841 gatatggggc actttggtaa gttccctatt cgcctggcgt ggttcaccgt cgtattacct
      901 tccttgaccc ttaattactt cggtcaggga gcgctgttgt taaagaaccc ggaagcgatt
      961 aagaacccgt tcttcctgtt ggcaccggac tgggcgctga tcccgctgct gatcatcgcc
     1021 gcactggcga cggtaattgc ctcgcaggcg gttatctctg gcgtcttctc attgacgcgt
     1081 caggcggtac gtctgggata tttgtcgccg atgcgcatta ttcacacctc cgaaatggag
     1141 tcagggcaaa tctatattcc gtttgtgaac tggatgctct atgtcgcggt cgtgattgtg
     1201 attgtcagct ttgagcactc cagcaacctg gcggcggcgt acgggattgc ggtgaccgga
     1261 accatggtgc tgacgtctat tctctcgact accgtggcac gtcagaactg gcactggaat
     1321 aagtattttg ttgcgctgat cctgattgct ttcctttgtg tcgatattcc attgttcacc
     1381 gctaacctcg ataaactgct ctccggcggc tggttgccat tgagcctcgg tactgtgatg
     1441 tttatcgtga tgaccacctg gaagagcgag cgtttccgct tgctgcggcg gatgcatgaa
     1501 catggtaact ctctggaagc gatgattgct tcgctggaga aatcaccgcc tgttcgcgtg
     1561 cccgggaccg cggtgtatat gtcgcgtgca atcaacgtca ttccctttgc gctgatgcat
     1621 aaccttaaac ataacaaggt attgcatgag agggtgattc tgttaactct gcgcaccgaa
     1681 gacgcgccat atgtccataa cgtccgtcgg gtacagattg aacaactgtc gcccactttc
     1741 tggcgcgtgg tggcaagtta tggttggcga gaaacgccaa acgtggaaga agttttccac
     1801 cgctgcggtc tggaaggatt aagttgccgg atgatggaaa cctccttctt tatgtcgcat
     1861 gagtcgttga tcctcggcaa acgcccgtgg tatttgcgtc tgcgcggcaa gctgtacttg
     1921 ctgctgcaac gtaatgcgct gcgtgcacca gatcaatttg aaatcccgcc aaacagggtt
     1981 atcgaactgg gtactcaggt cgaaatctaa cgctagacgc ctcctttctc cataaggggg
     2041 cgtttttgtt ttcatggtta atcaccatgt aaaacgtttc gaggttgatc acatttccgt
     2101 aacgtcacga tggttttccc aactcagtca ggattaaact gtgggtcagc gaaacgtttc
     2161 gctgatggag aaaaaaatga aaaaaggcac cgttcttaat tctgatattt catcggtgat
     2221 ctcccgtctg ggacataccg atacgctggt ggtgtgtgat gctggtttac ccatccccaa
     2281 aagtacaacg cgtatcgata tggcattaac ccagggtgta ccttctttta tgcaggtgct
     2341 gggcgtcgtc acaaatgaaa tgcaggtcga ggctgccatt atcgcggaag agatcaaaca
     2401 acataatccg caactccacg aaacgttgct cactcacctt gagcagctgc aaaaacacca
     2461 gggaaatacc attgaaattc gttacaccac gcatgaacaa ttcaaacaac aaaccgcaga
     2521 aagtcaggcg gtaattcgca gcggagaatg ttctccgtat gcgaatatca ttctctgtgc
     2581 tggcgtgacg ttctgaggcc gtcatggaag cattacttca gcttaaaggc atcgataaag
     2641 ccttcccggg cgtaaaagcc ctctcgggcg ctgcgttaaa tgtctatccg ggccgcgtga
     2701 tagcgctggt gggcgaaaac ggcgcgggta aatccaccat gatgaaagtg cttactggta
     2761 tctatactcg cgatgccggc acgcttttat ggctggggaa agaaacgaca tttaccgggc
     2821 cgaaatcttc ccaggaagcc gggattggga ttatccatca ggaactgaac ctgatcccgc
     2881 agttgaccat tgccgaaaac attttcctcg gtcgtgagtt tgttaatcgc tttggcaaaa
     2941 ttgactggaa aaccatgtat gccgaagcgg ataaattgct ggctaaactt aacctgtgct
     3001 ttaaaagcga caagctggtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg
     3061 ccaaagtgct gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga
     3121 ccgataccga aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccgcg
     3181 gtattgtcta tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg
     3241 tttttcgtga cgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc
     3301 tgattgagat gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc
     3361 cgggagatat ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt
     3421 ttactttacg caaaggcgaa attcttggcg tctctggttt gatgggcgca ggtcgtaccg
     3481 aactgatgaa agtgctctac ggcgcacagc cgcgcaccag cggttacgtc accctggatg
     3541 ggcatgaagt cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct
     3601 ccgaagaccg taaacgtgac ggtttagtgt tgggcatgtc agtaaaagag aacatgtcgc
     3661 tgacagcgct gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc
     3721 aggcagtgag tgatttcatt cgtctgttta atgtgaaaac accgtcgatg gaacaggcaa
     3781 ttggtctgct ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac
     3841 gccccaaagt gttgatcctt gatgaaccta cccgtggcgt agatgtcggc gcgaaaaaag
     3901 agatctatca actgattaac cagttcaaag ccgatggctt gagcatcatt ctggtgtcat
     3961 cggagatgcc agaagtatta ggcatgagcg atcgcatcat cgtcatgcat gaagggcatc
     4021 tcagcgggga atttactcgt gagcaggcca cccaggaagt gttaatggct gccgctgtgg
     4081 gcaagcttaa tcgcgtgaat caggagtaaa aaaatgacaa cccagactgt ctctggtcgc
     4141 cgttatttca cgaaagcgtg gctgatggag cagaaatcgc ttatcgctct gctggtgctg
     4201 atcgcgattg tctcgacgtt aagcccgaac tttttcacca tcaataactt attcaatatt
     4261 ctccagcaaa cctcggtgaa cgccattatg gcggtcggga tgacgctggt gatcctgacg
     4321 tcgggcatcg acttgtctgt aggttctctg ttggcgctaa ccggcgcagt tgctgcatct
     4381 atcgtcggca ttgaagtcaa tgcgctggtg gctgtcgctg ctgctctcgc gttaggtgcc
     4441 gcaattggtg cggtaaccgg ggtgattgta gcgaaaggtc gcgttcaggc gtttatcgct
     4501 acgctggtta tgatgctttt actgcgcggc gtgaccatgg tttataccaa cggtagccca
     4561 gtgaataccg gctttactga gaacgccgat ctgtttggct ggtttggtat tggtcgtccg
     4621 ctgggcgtac cgacgccagt ctggatcatg gggattgtct tcctcgcggc ctggtacatg
     4681 ctgcatcaca cgcgtctggg gcgttacatc tacgcgctgg gcggcaacga agcggcaacg
     4741 cgtctttctg gtatcaacgt caataaaatc aaaatcatcg tctattctct ttgtggtctg
     4801 ctggcatcgc tggccgggat cattgaagtg gcgcgtctct cctccgcaca acccacggcg
     4861 gggactggct atgagctgga tgctattgct gcggtggttc tgggcggtac gagtctggcg
     4921 ggcggaaaag gtcgcattgt tgggacgttg atcggcgcat taattcttgg cttccttaat
     4981 aatggattga atttgttagg tgtttcctcc tattaccaga tgatcgtcaa agcggtggtg
     5041 attttgctgg cggtgctggt agacaacaaa aagcagtaat aacgactaca ggacatcttg
     5101 aatatgaaca tgaaaaaact ggctaccctg gtttccgctg ttgcgctaag cgccaccgtc
     5161 agtgcgaatg cgatggcaaa agacaccatc gcgctggtgg tctccacgct taacaacccg
     5221 ttctttgtat cgctgaaaga tggcgcgcag aaagaggcgg ataagcttgg ctataacctg
     5281 gtggtgctgg actcccagaa caacccggcg aaagagctgg cgaacgtgca ggacttaacc
     5341 gttcgcggca ccaaaattct gctgattaac ccgaccgact ccgacgcagt aggtaatgct
     5401 gtgaagatgg ctaaccaggc gaacatcccg gttatcactc ttgaccgcca ggcaacgaaa
     5461 agtgaagtgg tgagccacat tgcttctgat aacgtactgg gtggcaaaat cgccggtgat
     5521 tacatcgcga agaaagcggg tgaaggtgcc aaagttatcg agctgcaagg cattgctggt
     5581 acatccgcag cccgtgaacg tggcgaaggc ttccagcagg ccgttgctgc tcacaagttt
     5641 aatgttcttg ccagccagca gattttgatc gcactaaagg tttgaacgta atgcagaacc
     5701 tgttgaccgc tcatccggat gttcaggctg tattcgcgca gaatgatgaa atggcgctgg
     5761 gcgcgctgcg cgcactgcaa actgccggta aatcggatgt gatggtcgtc ggatttgacg
     5821 gtacaccgga tggcgaaaaa gcggtgaatg atggcaaact agcagcgact atcgctcagc
     5881 tacccgatca gattggcgcg aaaggcgtcg aaaccgcaga taaagtgctg aaaggcgaga
     5941 aagttcaggc taagtatccg gttgatctga aactggttgt taagcagtag ttttaatcag
     6001 gttgtatgac ctgatggtga cataaatacg tcatcgacag atgaacgtgt aatataaaga
     6061 aaagcagggc acgcgccacc ctaacacggt ggcgcatttt atggacatcc cgaatatgca
     6121 aaacgcaggc agcctcgttg ttcttggcag cattaatgct gaccacattc ttaatcttca
     6181 atcttttcct actccaggcg aaaccgtaac cggtaaccac tatcaggttg catttggcgg
     6241 caaaggcgcg aatcaggctg tggctgctgg gcgtagcggt gcgaatatcg cgtttattgc
     6301 ctgtacgggt gatgacagca ttggtgagag cgttcgccag cagctcgcca ctgataacat
     6361 tgatattact ccggtcagcg tgatcaaagg cgaatcaaca ggtgtggcgc tgatttttgt
     6421 taatggtgaa ggtgagaatg tcatcggtat tcatgccggc gctaatgctg ccctttcccc
     6481 ggcgctggtg gaagcgcaac gtgagcgtat tgccaacgcg tcagcattat taatgcagct
     6541 ggaatcacca ctcgaaagtg tgatggcagc ggcgaaaatc gcccatcaaa ataagactat
     6601 cgttgcgctt aacccggctc cggctcgcga acttcctgac gaactgctgg cgctggtgga
     6661 cattattacg ccaaacgaaa cggaagcaga aaagctcacc ggtattcgtg ttgaaaatga
     6721 tgaagatgca gcgaaggcgg cgcaggtact gcatgaaaaa ggtatccgta ctgtactgat
     6781 tactttagga agtcgtggtg tatgggctag cgtgaatggt gaaggtcagc gcgttcctgg
     6841 attccgggtg caggctgtcg ataccattgc tgccggagat acctttaacg gcgcgttaat
     6901 cacggcgttg ctggaagaaa aaccattgcc agaggcgatt cggtttgccc atgctgccgc
     6961 tgcgattgcc gtaacacgta aaggcgcaca accttccgta ccgtggcgtg aagagatcga
     7021 cgcattttta gacaggcaga ggtgacgctt ggctacaatg aaagatgttg cccgcctggc
     7081 gggcgtttct acctcaacag tttctcacgt tatcaataaa gatcgcttcg tcagtgaagc
     7141 gattaccgcc aaagttgaag cggcgattaa agaactcaat tacgcgccat cagccctggc
     7201 gcgtagcctc aaactcaatc aaacacatac cattggcatg ttgatcactg ccagtaccaa
     7261 tcctttctat tcagaactgg tgcgtggcgt tgaacgcagc tgcttcgaac gcggttatag
     7321 tctcgtcctt tgcaataccg aaggcgatga acagcggatg aatcgcaatc tggaaacgct
     7381 gatgcaaaaa cgcgttgatg gcttgctgtt actgtgcacc gaaacgcatc aaccttcgcg
     7441 tgaaatcatg caacgttatc cgacagtgcc tactgtgatg atggactggg ctccgttcga
     7501 tggcgacagc gatcttattc aggataactc gttgttgggc ggagacttag caacgcaata
     7561 tctgatcgat aaaggtcata cccgtatcgc ctgtattacc ggtccgctgg ataaaactcc
     7621 ggcgcgcctg cggttggaag gttatcgggc ggcgatgaaa cgtgcgggtc tcaacattcc
     7681 tgatggctat gaagtcactg gtgattttga atttaacggc gggtttgacg ccatgcgcca
     7741 actgttatca catccgctgc gtcctcaggc cgtctttacc ggaaatgacg ctatggctgt
     7801 tggcgtttac caggcgttat atcaggcaga gttacaggtt ccgcaggata tcgcggtgat
     7861 tggctatgac gatatcgaac tggcaagctt tatgacgcca ccattaacca ctatccacca
     7921 accgaaagat gaactggggg agctggcgat tgatgtactc atccatcgga taacccagcc
     7981 gacccttcag caacaacgat tacaacttac tccgattctg atggaacgcg gttcggctta
     8041 gatttacgct gtcttttgat caaattatta ccatcggttg ttttcagaag catgaacatt
     8101 gctgctgaag caacagtaat aatgcccatc gtgataaacg tatagtggaa ttgttcgaca
     8161 gtcgttgtgc cttccatccc ttcataaacg cgaaggacgg ccgcacttac agcaacgcct
     8221 aaactaatcg acagttgctg cgtgaccacc agaacactgt taccgctgct ggcgttgtca
     8281 tcggtcagat cggcaagtgt gatggtattc atcgcggtaa attgcgtcga catagccatc
     8341 cctaatataa acaacggcaa gatcagcatc catatcgcca ttgccggtga ttgcaaagag
     8401 aactgagcga tcattagccc aataatcacc gtgatcccca ctaacgtatg gcgatagccc
     8461 agacgacgta agacttgggt aaccatcgat tttgcaataa tggaacctaa cgctgtcggt
     8521 gccatcatac agccggcaat aaacgcctga taaccaaatc ctacctgtaa catcaatggc
     8581 ataaggaacg atacacaacc ggtccccaga cgggttgcaa tattgcctac gataccgatt
     8641 gagaaagtgc gggttttaaa taaatctaat gaaattaatg ggtttggcgt gtgtcgcgca
     8701 tggagaatat agagaagcag taacccgatg ctggtgacaa ttaccgtcaa ggcaatccag
     8761 ctggcgacaa tcttttcccc gaatagctct attccgcttg agaagagaac aaggctgagg
     8821 ccaaacagca aaaagccagt gatatcgaat ctgcgtcgtg cggtggtgaa attgggcata
     8881 tgtttgcgcg cgtaaagaag gcccgcaata cctatgggga tattgattaa aaatatccag
     8941 tgccaggttg cccaggtgac cagcacgccg ccaagaacgg ggcctaaaat tggccccacc
     9001 agacccggca tggcgacaaa attcaatact ggaagaagtt cattacgagg ataagcgcgc
     9061 agtaaggcca gccgagcaac aggcatcatc attgcgccgc ctatcccctg aataacccgg
     9121 aagacaacca gctgtggtag cgaattagaa agtgcgcagg ccagagaacc caatgtgaac
     9181 agactcacgg caagggtaaa aatgcgacgc gtaccgaagc gatcggctag ccatccgctt
     9241 accggaataa gcatcgccac cgtcagcgta taactgatga tggctgattg catcgcgaga
     9301 ggagaacgat taaggctatg agcgattgcg ggtaaggcgg tattcagaat agtggcatca
     9361 agtgcctgca tgaagaaggc catcgccgcg atccacggca aacccgccat actgcgcttc
     9421 tttttatcgc tcattcaatg tcttgttatc gggttgtcac ttatcaggtg agcgtagcag
     9481 cgcctgacaa gctttaaatg ccgcgtcgcc atcgctttgg acaatcgcat cgacaatcgc
     9541 ctggtgcaga tttagcttta tcactgtgtc gctggtaatt gacgtgaagt aagtgtgata
     9601 aaccgaatgg aatagcgagg cgaatgaggt caaaaacgga ttggcgctca tttcatagat
     9661 atgctcatgc caggccatat cgacttcgat ccagcgttca cggcgaaagt tctcttttaa
     9721 tgccgccatt tcggccatta acgtattgag atgcgccttc tgttccgcgg tgccaaccgt
     9781 tgctgccagt aggcaggctt gcggctccag acagatacgc ataaccagaa agtgatcgat
     9841 gacctgatga aagttctctt ctgtcatcca ccaggtaagc aattcctgat caagaaaatt
     9901 ccagttcgat tgtggcatga cccgagtacc aattcgcggt cgcggtaaaa ccatcccttt
     9961 tgccgttaac gttttgaccg cttcgcgtac cgctgtacga ctcactccaa attgctcgcc
    10021 cagctcaatc tcaccaggca aaatggtgcc gggttcatat tcacctttta agatccgttg
    10081 cgccagcttc tcagccagaa catacgaaag gtttttctgt gcagctaact gttgtgcgct
    10141 taaaggcatt acttatcttc ctttttcttt ttattcctcc ttagtatgcc accaggaagt
    10201 gtgattacgg ttgcaaaaac ggcaaattgc ttgttttatg gtacattaac ggggcttttg
    10261 ctgaaaaaat gcgcggtcag aaaattattt taaatttcct cttgtcaggc cggaataact
    10321 ccctataatg cgccaccact gacacggaac aacggcaaac acgccgccgg gtcagcgggg
    10381 ttctcctgag aatctcaaca gagaaagcaa aaataaatgc ttgactctgt ggcgggaaag
    10441 cgtattatgc acacc
//
LOCUS       DAZPDU010000155        10252 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZPDU010000155 DAZPDU010000000
VERSION     DAZPDU010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10252
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.157"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            113..445
                     /locus_tag="ACHURS_003485"
     CDS             113..445
                     /locus_tag="ACHURS_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFR1239130.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            592..1953
                     /gene="yegQ"
                     /locus_tag="ACHURS_003486"
     CDS             592..1953
                     /gene="yegQ"
                     /locus_tag="ACHURS_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFR1239131.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(2283..2600)
                     /gene="yegR"
                     /locus_tag="ACHURS_003487"
     CDS             complement(2283..2600)
                     /gene="yegR"
                     /locus_tag="ACHURS_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFR1239132.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            3006..3905
                     /gene="yegS"
                     /locus_tag="ACHURS_003488"
     CDS             3006..3905
                     /gene="yegS"
                     /locus_tag="ACHURS_003488"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFR1239133.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDGIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCLLLR"
     gene            complement(3987..4760)
                     /locus_tag="ACHURS_003489"
     CDS             complement(3987..4760)
                     /locus_tag="ACHURS_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR1239134.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(4866..5906)
                     /gene="gatD"
                     /locus_tag="ACHURS_003490"
     CDS             complement(4866..5906)
                     /gene="gatD"
                     /locus_tag="ACHURS_003490"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFR1239135.1"
                     /translation="MKSMVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTYPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(5954..7309)
                     /locus_tag="ACHURS_003491"
     CDS             complement(5954..7309)
                     /locus_tag="ACHURS_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFR1239136.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSTQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKTVLA
                     E"
     gene            complement(7313..7597)
                     /gene="gatB"
                     /locus_tag="ACHURS_003492"
     CDS             complement(7313..7597)
                     /gene="gatB"
                     /locus_tag="ACHURS_003492"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFR1239137.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHSIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            complement(7628..8080)
                     /gene="gatA"
                     /locus_tag="ACHURS_003493"
     CDS             complement(7628..8080)
                     /gene="gatA"
                     /locus_tag="ACHURS_003493"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFR1239138.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVEVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(8090..9347)
                     /gene="gatZ"
                     /locus_tag="ACHURS_003494"
                     /pseudo
     CDS             complement(8090..9347)
                     /gene="gatZ"
                     /locus_tag="ACHURS_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
     gene            complement(9376..10230)
                     /gene="gatY"
                     /locus_tag="ACHURS_003495"
     CDS             complement(9376..10230)
                     /gene="gatY"
                     /locus_tag="ACHURS_003495"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFR1239139.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTYAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADVFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
BASE COUNT         2707 a         2649 c         2323 g         2573 t
ORIGIN      
        1 actgacatta tctctccgcc ctggcaggca tcagaaaatc gctcatactt taatcggtaa
       61 acagcacctt tagatgctgt tttcgatacg caatttcaat caaggagtca ttatggctgg
      121 ttggtttgaa ctcagtaaga gcagtgataa tcagttccgg tttgtgctaa aagcgggcaa
      181 tggtgagact atcctcacca gcgagctgta tacatcaaaa gcctctgcgg aaaagggcat
      241 cgcgtcggtg cgtagcaaca gcccgcaaga agaacgctat gagaaaaaaa cggcaagtaa
      301 cggcaaattc tatttcaatc tgaaagccgc caatcatcaa attatcggct ccagccagat
      361 gtacgccacc gcgcaatctc gtgaaaccgg aattgcctcc gttaaagcca atggcacaag
      421 ccagacggtg aaagacaata cgtaattacg atgccgggcg tgggttgtcg gcgtccggcg
      481 ctttaccaga tccccccaca gcgctacaat gcccgccctt aaagtggggg cactccccta
      541 accgcttcat caggtgaagc ggagctgacc tgtcatcaga acgagagaat tatgtttaaa
      601 ccggaactcc tttccccggc gggaacgctg aaaaatatgc gttacgcttt cgcttatggc
      661 gcagatgctg tttatgcggg ccagccgcgt tactccctgc gtgtgcgcaa caacgaattc
      721 aaccacgaaa atcttcagct cggcatcaat gaagcccacg cgctggggaa aaagttttat
      781 gtcgtggtca acattgcacc gcacaacgcc aagctgaaaa cctttatccg tgacctgaaa
      841 ccggtggtgg aaatggggcc ggatgcgctg attatgtccg atccagggct gattatgctg
      901 gtgcgtgagc acttccctga aatgccgatc cacctctcgg tgcaggctaa cgccgtaaac
      961 tgggcgacgg tgaaattctg gcagcaaatg ggcctgaccc gcgtgatcct ctctcgcgag
     1021 ctgtcgctgg aagagattga agagatccgc aatcaggtgc cggatatgga gattgagatc
     1081 ttcgttcacg gtgcgctgtg catggcctac tccggtcgct gcctgctctc tggctatatc
     1141 aacaagcgcg atccgaacca gggtacctgc accaacgcct gccgctggga gtacaacgtc
     1201 caggaaggga aagaagatga cgttggcaac atcgtacaca agtacgagcc gattccggtg
     1261 caaaatgttg agccgacgct aggtatcggc gcgccaaccg acaaagtgtt tatgatcgaa
     1321 gaagcccagc gtccgggcga gtatatgacc gcgtttgaag atgagcacgg cacttacatc
     1381 atgaactcga aagatctgcg cgccatcgcc catgtagaac gcctgaccaa aatgggcgtg
     1441 cattcgctga aaatcgaagg ccgtactaaa tctttctact attgcgcacg caccgcacag
     1501 gtttatcgta aagctatcga tgatgccgct gcgggcaaac cgttcgatac cagcctgctg
     1561 gaaactctgg aaggtctggc gcatcgtggc tataccgaag gtttcctgcg tcgtcatact
     1621 cacgacgatt atcagaacta cgaatacggt tattcagttt ctgaccgcca gcagtttgtt
     1681 ggtgagttta ccggtgagcg caagggggac ctcgcagcgg tagcggtgaa aaataaattc
     1741 tccgttggcg acagccttga gctgatgacg ccgcaaggca acattaactt tacccttgag
     1801 cacatggaaa acgccaaagg tgaagcgatg ccggtcgcac caggcgatgg ttatactgtg
     1861 tggctcccgg tcccgcagga tcttgagcta aattacgcgc tgctgatgcg taatttctcc
     1921 ggggaaacca cgcgtaaccc acacggtaag tgattaattt cgattatttt tcccggatgg
     1981 aaaattctta gaaaccgatc acatacagct gcatttatta aggttatcat ccgtttcgct
     2041 gaaaaacata acccataaaa tgctagctgt accaggaacc acctccttag cctgtgtaat
     2101 ctcccttaca cgggcttatt ttttatgcat aagccctatc cctggtcacc gtcttccatt
     2161 gaccacatcg atagaatctc ccttcatagc acgatgcctt tcacgtaacg gcatcgtgct
     2221 cgcacaggtt ccggctaagc acaaccagaa cgcgcatgtt tgatgcttac caaaaaatat
     2281 tctcactctc cacatttgaa tgtcagacga gcgacgccat gtaatcctgc accttctgtc
     2341 ttcaggtcaa ctatctgcat ttttttgccc tgagtaacac agaaatgggc tgcatcattt
     2401 tttactatat tttctgcacc agatattctg cccctagcta aagaagcttc ggcttcggtg
     2461 tagtattggt tatcgagttt acgctgaata ttacttttat atgcaagacc aaatttaccg
     2521 atacttgttt catcattatg cacagcacaa ccagatataa gaaaaatact aattaatgat
     2581 atagcagcta tctttttcat ctcaccttcc cccattaaat accaacgaca ctctctagtg
     2641 tttaaatata ataatggcat gattattata attgaatagg attataataa atgttctgta
     2701 caacatttcc tacataagta ggaattacgg acattgaggc ccttcagggt aactccatgg
     2761 gggctttaat atattatatt gaagatgcca ctgtttagtt gaatattagg tatatgctct
     2821 tttttgaaat ttatcggtgg cagccgttag tattcgctgt ccccattgca agctcctggt
     2881 ggtaaccact gaatcctcca tacttgaact gactttttat cctccgactt tcatcctgtt
     2941 ctgactccac cttttgtttt ctgctctaca ctatctacag accaatcata aaggcacata
     3001 cgatcatggc agaatttccc gccagcttac tgattcttaa tggcaaaagt actgacaatc
     3061 tacccttgcg cgaagcaatt atgctgttgc gtgaggaagg aatgacgatc catgtgcggg
     3121 tcacctggga gaaaggcgat gccgcacgat atgtagagga ggcccggaag ttgggcgtcg
     3181 caacggtgat tgccggtggt ggcgatggca ccattaatga agtttctacg gcgttgattc
     3241 agtgtgaggg ggatggcata cccgcgctgg gaattttgcc attaggaacc gccaatgatt
     3301 ttgccaccag tgtagggatt cctgaggcac tggataaggc gctgaaactg gcaattgccg
     3361 gtaacgccat tgcgatagat atggcgcagg tcaacaaaca aacctgtttt attaatatgg
     3421 cgacaggcgg atttgggacg cgtattacca cagaaacgcc ggaaaaatta aaagccgcgc
     3481 tgggtggcgt ctcttacatc attcatggct taatgcgcat ggatactctg caaccggacc
     3541 gttgtgaaat ccgcggtgaa aactttcact ggcaaggtga cgccctggtc attggtattg
     3601 gtaacgggcg tcaggccggt ggcggtcagc aattgtgtcc gaacgcgtta attaacgatg
     3661 gcttgctgca actgcgcatt tttaccggcg atgaaattct tccggctctc gtatcaacct
     3721 taaaatctga cgaagataac ccgaatatta tcgaaggcgc ttcgtcgtgg tttgatatac
     3781 aagccccaca cgaaatcact tttaatcttg atggcgaacc gttgagcgga caaaactttc
     3841 atattgaaat acttccggcg gcgttgcgtt gtcgattacc accagattgt ctattattgc
     3901 gttaatcaaa ctattctctc ttgaaccagg gagaatccga aatgccggat gcggtgtaaa
     3961 taccttatcc ggcatacaga acaatactac accgtaatta attttacccg tgtcttctgc
     4021 aatgccagtt tatccacctc tgatacagca tcatcggtaa taataatgtc aattttttcg
     4081 attggcagta cctgattaaa accgcgacgg ttaaatttcg atgaatcgag aacggcaatc
     4141 actttattag cggcggtgac catcgcaccg ctaatcgaat aaccttcatt aaaggtcgta
     4201 ataccattaa ccgcatcaat accatcagca ccgacaaaca ttaaatcggc attaatatcc
     4261 tgcaatgaac gctcggcaat ggaaccatgc atcgagcgcg ttttatggcg gaccgtgcca
     4321 ccacagacga ccagagtaat gtctttattt tcggaaaggg caaacgctgc cgggagactg
     4381 ttggtaatca ccgtgatatt tttagcggtc attaatcctt cggcgataag cattgtcgta
     4441 cttccgctgt cgagaataac ggtcatccct tcatggatca ttttgactgc cgcctgcgct
     4501 attctgtttt ttggcgcgct ggcgagctga aatcgctctt taaacccgac ttcctgggtc
     4561 tcggtttcac tattaccgga cattattttc gccgcaccgc catgaaagcg cgtaaccacg
     4621 cctttttgtt cgagaaagcg caaatcggca cggattgtcg cttccgaggc agcaaatact
     4681 cccgccagat cctgaacaag cacggttccc tgttcattca ctaattggat gatcttattc
     4741 cttcgctcga atgaattcat cgtcatttac ccatattcaa ttgtggctag tgtaaacgaa
     4801 gggggaccat tggtgaatga tctgttttgc acaataccgg gccagcatca cgctggcccg
     4861 cggtttcatg gaatgagcaa cactttgccc ggcatggcat tacgagcgat gtcacgcacc
     4921 gcctgggtga agctttcaaa gcttccacgg tgagcgatta atggctccag gcttaactta
     4981 cgttctgtca gcaaccggct cgccgtttcc cactcctgcc ccggccaagg gctggaatag
     5041 ttcatccaac tgccgataac cgtcagctct ttacgcaata ttttgccaaa cgttgccgat
     5101 gttaaatgca gatcctgatg caacgtgccc accagcgcca gttgggcatg aggaccggca
     5161 atctctaccg ccagttcgac agtttgcggt acgccagccg tctcgaggat aagctgatta
     5221 aagcgcagtt cgcgtaaaac gctctgcatt tgcggcgcgc tcatttcgct actgttaaat
     5281 gtttgcatcg caccgaaaga ttttgccaat gccagttttt ctgagctaat gtcgatcgcc
     5341 gtcacactct ttgctcccag cgcgacagcg cactgaatag ccagcaggcc aatggttccg
     5401 gcaccaataa taataacgtt tttattctca caaccttgcg ctaaatgaaa agcatgcagg
     5461 ccaacggtaa tcggctcaat aaaagcccca tcctcaatag gcatatccgt gggtagagca
     5521 aagacatttt ttcgcttaac gacaatatat tcagcaaatc caccatcacg ccgcgagcca
     5581 ataaaatcat attttgcgca ctgggaataa aaccctttca aacactctgg ataagtaaaa
     5641 cagggtaata acggcacaca ggcaaccgca tcgccagggt gtaaatcatc aacaccggat
     5701 cccaccgcat caatatagcc gctaaattca tggcctaacg ttattggata ataatgtgca
     5761 ccatttttaa atatcctggg taaatcggaa ccacataagc ccgagctggc aatttttacc
     5821 cgcacctcat cctgatgttt aatttcagga atgacgcttt ctgcaacgcg cacgatacca
     5881 tcagtatcat tcaccattga tttcataaaa actcctgatt gttaaggggg ataacctccc
     5941 cctcaggtaa aaattattct gcgagaacgg ttttctcttg tttaataaaa ccacgcgctc
     6001 tacgccaggt catgaaaata ccggtaagat aaattgcgcc gataataatg aaaccgggaa
     6061 tattttgcgt ggagaaaacc tgaatcagta accaggtaat gggagaaccg ccctgatcca
     6121 ttgaagccac catacccccg gcttttaacg ccccagcatt agccgccagt tgggtgtgca
     6181 aaccaatagt ttgcgtcgcg atccacaggg tgatgctcat aatgatgaca cccgagatta
     6241 aggtgcggaa cagatttcca cgatgcaccg cgaccgccat cgccacgaag aagccgatgg
     6301 tggcaagatc gccaaacggc agtacctgat tacccggaac acaaacagca attaaaatgg
     6361 tgagcgggat aaaaatcagg cttgccgata ccaccgccgt atgccccagc agtaatgcgg
     6421 gatccaggcc aatcaggaac tcctgaccgc cgaacttcgc ctgtaaacga ctacgggcct
     6481 gcttagcgat gggcgttaaa ccatccatga tgggtttaat cacccgtggc atcagcagca
     6541 taactgccgc cgtttttacc gccagctgca atacaccttt gacatcgtaa cccgcgagga
     6601 tgccgataat cagccccatc acaaaaccca cggtgacagg ctcgccaaat ggaccaaatt
     6661 tgcgctgaat atcgtcggcg ctaaatttaa ttcggttaac gcctgggatt ttctcgatga
     6721 tagcatcgac cagcaccgca atcggcccca tatacgccga cgtaccgtgc ggaatagcaa
     6781 taccttccag ctcaaagaaa tttcgggtat cgcgggcaaa ccagtcgccg agcttataaa
     6841 caaacgccgc gtgaattacc acacctgcca tccctatcat ccatgaaccg gttgccagat
     6901 gcagcaacgc gccggtgaag gtcatatgcc agatattcca gatatcaaca tttaccaccc
     6961 gcgtcatacg ggtcagtaac atcgccacgt taaccagaat cgcaatcgga atcgccacca
     7021 gcgcaatttg cgacgcccag gtcattggtg aagagcccgg ccagccgaca tcgaccacat
     7081 gcagattcag gtcgaaattt tccgccatcg ctttcgccgc cggaccaatg gaatccagca
     7141 ttaagccaat cacaaggcca atgccaacaa agccaatccc gatatgcaga cccgctttaa
     7201 agcaatcgcc tgccttcatg cctaatattt tagaaaaaat aatgatgaca atcggaagca
     7261 tcaccgtagg gccgaggtcg agaatataac gcatgacttc tgaaaacata ggtcacccct
     7321 gtaagatagt cagaatttta ttttgtaatg cttcgatacc gacaccagaa ataaaaggca
     7381 tgccgtgaac taacggaata tcgccaaaac tacgatccac tttggcagtg gtgcatatca
     7441 aatgcacacc atccatatag gtttctattt cattaacccg acactggatt aattcaacag
     7501 gaatactatg attctgacac aactctttaa tttcttccgc cgccatcgta gaggtcgcaa
     7561 ccgcgcctcc gcaagcgaca ataatcttgc gtttcataaa tagtcctttt tattgttaca
     7621 gagggattta ttcgtctgaa tctaaaacat actttgtgaa gtattccttt aactgggttt
     7681 caggaagagt gattagtgtc tcgacgatat cgggctgttg taacttgcca aataaacagc
     7741 gtaaaagttt caattgctgc tgcggattct ccacaatcaa cgcaataacc aacgatacct
     7801 ccacgtcgtt atcatcatcc gcttgctgaa aatgaacttt atttgttggc cttaacagat
     7861 aaatggctga cgacttagca tgaatcgcct cacaatgcgg tattgcaatg gcgtgctgct
     7921 caagcattat cccggtaggg aattctgctt ctctggcaat taatgcctgt ggccaggtat
     7981 catgaaccac acctttggcg agcatctcat taccgatatg ggttaaaact tcgctacgat
     8041 cgacaaaaga aattccgcta cgaacaaaca ggttagtcat ataccgtcct tattccgcac
     8101 agccgtagcg ataggcgcgc aaaacatcat aaattttatc cataatcagc tgatgcggta
     8161 ttgctgataa ttccccggac tgaatgcgtt caaattgttt gggaagatac tgactaatca
     8221 tgcccagtgg gatgtccacg ccttcaagat tcaccatcat cgtttcgacg ctatttttaa
     8281 tccgactatg cggccagtaa taacgaatac gatccgacag gctgtaacga atatccagta
     8341 atgaatcgtt aaaacccgta cgataatatt ttttccagta ttgcggttcg tcgagcatca
     8401 cttcttcaat taccgccagg caaccgctgc gattttcagg ggcgataagt tcctgctcaa
     8461 tttgtgccag tgcaaatatc gcctcgcgta aagcaaaggt taatgcggga ccgactttca
     8521 atattgcaaa gtgatcgcgg actaattccc aataagccgt ccgggtctgg taatcggtag
     8581 aatgtgcttc ataaaccatt cgggtgtttt ctatccattg cgccagcggc tgcgcttcct
     8641 gcggctgata atggataata ttgctgtgat caaattccac gcccggctgc accacgatgg
     8701 caatcacgcg tgttaacgcc tctgtcagcc cacgggcaat aaaggccttt tgatgcgtac
     8761 gtaaagtatt ggcggcatct tcaacatggg tgatgtgtac tgactgaatg gcgctggcct
     8821 caccgcccgg aaccggaact tcagtgccaa tgacatagct cagttgctca cgctggcaat
     8881 ctgtcgccac actttccgca gcaaagcaaa gcagctcgtt ccgcaaccgt ttctggtgct
     8941 aacggtatgg gatcccccgc gcaggacatt gacgcatcaa gatgaatttt actgaagcca
     9001 gcacgaacat atgcttttac cagctcgacg gatttttcca tcgccgcatc cgcattttct
     9061 tgctgccagc agtttggccc cagatgatcg ccgccgagaa taatgcgttc gcgtgcaaat
     9121 ccgactttat cggcaatcgt aaaaacaaat tcgcgaaagt ctgccggtgt cattccggta
     9181 taaccgccaa attgattgac ctggtttgac gttgcttcaa tcagcacttt gcgcgtgctg
     9241 ttgcgatcaa atgccagcgc cgcttcgata accaacggat gggcagaaca gactgaacat
     9301 atgccgatat gttcaccagc tttatgccgg gcaattaacg ttttcatgtt ttttccttgt
     9361 taaatggcag gtgcgttatg ccctgccctc gcagccacaa tcggcaatca ctttgctcac
     9421 cacatcgcgc attgcggatt tagccgactg caaataatcc cggggatcgg tcgcttcagg
     9481 gtgctcggtc aggtaatttt ttaacgcctg cgagaaggca tttttcagct ccgttgcaac
     9541 gttgattttg catatcccca gtttgatggt ttgctgaata tccttagtcg ataaccctga
     9601 cgcgccatgc agcaccagcg gtaagttcac ccactggcga atgttctcca gtcgagaaaa
     9661 atcaagcgcc ggtgcgctgg catacatccc atgagccgtg ccgatggcga ccgccaggga
     9721 atcaattccg gttgcctcgg caaattcacg cgcctgagcg gggttggtat aaaaaacatc
     9781 ggcttcattg acttgcacat catcttcctg gccgccaagt tgccccagct ccgcttcgac
     9841 gctgacatca aagcgatggc aaaaatccac cacctctttg acccgtgaaa tattttgcgc
     9901 aaaaggcaaa tgcgaggcgt caatcatgac tgagcgcacg ccagaacgaa ccttctgagc
     9961 gatatcgtca aatttcgtgt gatggtcgag atgaattgcc agtggatggt gatattgctt
    10021 cgccatcgcg ctgaccagcg ccaacagatt ttctgtacca gcataagtga atgtgccagg
    10081 cgttccggcg atgatgaccg gcgcatgcag gttggcagcg gtttccacca ccacttgcat
    10141 cgtttcgaga ttgtgaatat tgaatgccgg aaccgcataa ccgccgcgct gtgcgttgtt
    10201 cagcatctgc tttgtcgata ccacgtacat tttcatatcc tgtcgcttgt tt
//
LOCUS       DAZPDU010000156        10148 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZPDU010000156 DAZPDU010000000
VERSION     DAZPDU010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10148
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.158"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(61..136)
                     /locus_tag="ACHURS_003496"
     tRNA            complement(61..136)
                     /locus_tag="ACHURS_003496"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(101..103),aa:Lys,seq:ttt)
     gene            complement(170..245)
                     /locus_tag="ACHURS_003497"
     tRNA            complement(170..245)
                     /locus_tag="ACHURS_003497"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(210..212),aa:Lys,seq:ttt)
     gene            complement(392..467)
                     /locus_tag="ACHURS_003498"
     tRNA            complement(392..467)
                     /locus_tag="ACHURS_003498"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(432..434),aa:Lys,seq:ttt)
     gene            complement(471..546)
                     /locus_tag="ACHURS_003499"
     tRNA            complement(471..546)
                     /locus_tag="ACHURS_003499"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(511..513),aa:Val,seq:tac)
     gene            complement(696..771)
                     /locus_tag="ACHURS_003500"
     tRNA            complement(696..771)
                     /locus_tag="ACHURS_003500"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(736..738),aa:Lys,seq:ttt)
     gene            complement(936..1727)
                     /gene="cpoB"
                     /locus_tag="ACHURS_003501"
     CDS             complement(936..1727)
                     /gene="cpoB"
                     /locus_tag="ACHURS_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFR1239140.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(1737..2258)
                     /gene="pal"
                     /locus_tag="ACHURS_003502"
     CDS             complement(1737..2258)
                     /gene="pal"
                     /locus_tag="ACHURS_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFR1239141.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(2293..3585)
                     /gene="tolB"
                     /locus_tag="ACHURS_003503"
     CDS             complement(2293..3585)
                     /gene="tolB"
                     /locus_tag="ACHURS_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFR1239142.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(3718..4950)
                     /gene="tolA"
                     /locus_tag="ACHURS_003504"
     CDS             complement(3718..4950)
                     /gene="tolA"
                     /locus_tag="ACHURS_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFR1239143.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALLWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKIKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAAAEKAAA
                     DKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEKAAADKKAAAAKAAAEKAAA
                     AKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAGQIK
                     SAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKP
                     PSQAVYEVFKNAPLDFKP"
     gene            complement(5015..5443)
                     /gene="tolR"
                     /locus_tag="ACHURS_003505"
     CDS             complement(5015..5443)
                     /gene="tolR"
                     /locus_tag="ACHURS_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFR1239144.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(5447..6139)
                     /gene="tolQ"
                     /locus_tag="ACHURS_003506"
     CDS             complement(5447..6139)
                     /gene="tolQ"
                     /locus_tag="ACHURS_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFR1239145.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(6136..6540)
                     /gene="ybgC"
                     /locus_tag="ACHURS_003507"
     CDS             complement(6136..6540)
                     /gene="ybgC"
                     /locus_tag="ACHURS_003507"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFR1239146.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(6690..6983)
                     /gene="ybgE"
                     /locus_tag="ACHURS_003508"
     CDS             complement(6690..6983)
                     /gene="ybgE"
                     /locus_tag="ACHURS_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFR1239147.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(6983..7096)
                     /gene="cydX"
                     /locus_tag="ACHURS_003509"
     CDS             complement(6983..7096)
                     /gene="cydX"
                     /locus_tag="ACHURS_003509"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFR1239148.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(7111..8250)
                     /gene="cydB"
                     /locus_tag="ACHURS_003510"
     CDS             complement(7111..8250)
                     /gene="cydB"
                     /locus_tag="ACHURS_003510"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFR1239149.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(8266..9834)
                     /gene="cydA"
                     /locus_tag="ACHURS_003511"
     CDS             complement(8266..9834)
                     /gene="cydA"
                     /locus_tag="ACHURS_003511"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFR1239150.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT         2485 a         2743 c         2439 g         2481 t
ORIGIN      
        1 ggttcgaatc tttgaatatc tcagatagca aaaaagcgcc tttagggcgc ttttttacat
       61 tggtgggtcg tgcaggattc gaacctgcga ccaattgatt aaaagtcaac tgctctacca
      121 actgagctaa cgaccccttg cgggattatg ctgctttaat cattcaggat ggtgggtcgt
      181 gcaggattcg aacctgcgac caattgatta aaagtcaact gctctaccaa ctgagctaac
      241 gacccatacg ggtgttacct gaaagatttc tactcggggc caatctttaa gattggtggg
      301 tcgtgcagga tgactcgctt cgcttgccct acgggccgtt gctggcgcaa cgttatcctt
      361 cacgcttaac atctgagttt gatgttaaaa ttggtgggtc gtgcaggatt cgaacctgcg
      421 accaattgat taaaagtcaa ctgctctacc aactgagcta acgacccgag tggtgggtga
      481 tgacgggatc gaaccgccga ccccctcctt gtaagggagg tgctctccca gctgagctaa
      541 tcacccgata ctgcgctgga tactacaaac tacttccacc gaacccaccg taattagtgg
      601 tgggtcgtgc aggatgactc gcttcgctcg ccctacgggc cgttgctgac gcaacgttat
      661 cctacacgct taacatctgt cttcgatgct aaaactggtg ggtcgtgcag gattcgaacc
      721 tgcgaccaat tgattaaaag tcaactgctc taccaactga gctaacgacc cactttttcg
      781 ttgctttcgg tttgtttgat atcccgtggc aacggcggca tatattactg atttcagact
      841 tgagcgcaac aaaaatttcg atgcagatca ctcaactgct tatgattcgc acgacacgac
      901 cagaaataat gcgacttctg gtcgtgtgtt atgcattaca tcgcgttcag acgtttttgt
      961 gcctgtttag cgccatcagt accagggtat ttactgataa cctgctggta tacggctttc
     1021 gcttttgcgg tgtcaccttt gtcctgcatg atgacgccga ctttaaacat cgcatcagca
     1081 gcctttggtg acttcggata gtttttcact accgaagcaa aatagtacgc cgcatcatct
     1141 tttttaccct tgttgtagtt taactgaccc agccaataat tggcgtttgg caggtaagtt
     1201 gaatcagggt aatttttgat gaaattctga aatgccacca ttgcgtcatc ctggcgggat
     1261 ttatcctgca ccagcgcaat agctgcattg taatccgtgt ttgcatcgcc gcttttcacc
     1321 ggcgcgccag cattcgcagt accagcatca gctgtcggcg tcgttgatgc cgccgcaccg
     1381 ctttgatcgc tgctggttga ttgcgccgct gcaccaccgc tgctgaggct gtcgatctgc
     1441 aacaggatct gcttttgccg ctccacgacc tgattcagtt gatactgatt ttcctgaatc
     1501 tgaccacgca gggaatcaat atcggattga ttatcagaaa gttgttgctg gagttgggtt
     1561 aaaagctggc tgtgagcatt agaaatacgc tcaagttgag tgacgcggtc ttcgaccgag
     1621 cctgagccga cactactgat tggtgcctga gcaaaagcgg cccagggggc cgctatacca
     1681 accagtaacg acagactcaa tagttgatgt ctgaagttac tgctcatgca attctcttag
     1741 taaaccagta ccgcacgacg gtttttggag tatgccgctt cgtcatgacc cagtactgca
     1801 ggtttttctt taccgtaaga aacgatggag atctggtctg cagaaacgcc tttaccctgc
     1861 aggtacatct taacggcgtt cgcacgacgt tcacccaggg agatgttgta ttccggagta
     1921 ccacgttcgt ccgcgtgacc ttctacggtg actttgtaag acgggttgct acgcaggaag
     1981 tttgcatgtg catccagcat ttgagcgaag tcagaacgga tatcgtactt gtccagatcg
     2041 aagtaaacga tgttgttctg ctgcagctgt tgcatttgca gacgagcctg ctcttcggaa
     2101 gacatgttgc cgttgccgcc gttcgcatcc ataccagtgc cggcacccag catgccttcg
     2161 ctgccgtcat tgctggcgtt cttgttggaa gaacatgccg caattgccat aacaggcaga
     2221 gcaatcatca gccctttcag cactttgttc agttgcattt caatgattcc tttactattc
     2281 aattaattat tatcacagat acggcgacca ggcagggaat ttgacctgtc catcagttgc
     2341 cggaagacgc gctttgaaac gcccatctgt agaaaccaaa ttcagcacgg atcccatccc
     2401 ctgagaagag ctgtagatta ccatagtgcc gttaggtgcc agacttggcg tttcatccag
     2461 gaacgtggac gacagaactt gtacgcctcc cgttgccaga tcttgtttgg caatgtgctg
     2521 ctgcccaccg ttggagctga ccattaccat aaatttaccg tcgctgctga catccgcatc
     2581 ctggttctgc gaaccttccc aggtaatacg ttgtggcgca ccgccgttga tattcacttt
     2641 ataaacctgt ggacgaccgg cctggtcaga agtaaatgcc aggttctggc tgtccgggaa
     2701 ccaggtcggt tcggtattgt tactgcgacc atcagtcacc tggcggatct gaccagaagc
     2761 caaatccatt acgtacaggt tcaggctacc ggttttcgac aaggcgaatg ccagtttgct
     2821 gccgtctggc gagaatgcag gcgcaccgtt gtgacgcggg aatgaagcca cctgacgtac
     2881 agcgccgttt gccagcgttt ggataactag cgcggaacga ccgctttcga aggtcacata
     2941 agccagttta gaaccgtctg gtgaccacgc cggggacatc agcggctgtg gtgaacggtg
     3001 aacgacaaac tggttgtaac cgtcatagtc agatacgcgc agttcatacg ggaactgacc
     3061 gccgttggtc tgaacaacgt aggcaatacg ggtacggaac gcacctttaa tgccggtcag
     3121 tttttcaaac acttcatcac tggcggtatg accagcatag cgcagccact gcttgttcac
     3181 tttgtacgag ttctgagcaa gtacagtacc cggtgcgccg ccagtgtcaa caagttgata
     3241 agcaacattg taagaaccat ccggattcgg agtgacctga ccgacaacta cagcgtcaat
     3301 acccagcgcg gaccatgcag ctggttgtac ttcctgcgca ctacccggct gctgtggcag
     3361 acgagcgcga tctaacggat taaatttacc gctgttacgc aagtcggcag caacgatgcc
     3421 gccaatatct tcaggtgccg caccagggcc cgcccactgg aaaggaacaa caccaatagg
     3481 acgaccggaa tctacaccgc tgtcgatcac aatgcggact tcagcatgca gaactgatgc
     3541 ccacagtatg agaaaaccaa atgctactcg taatgcctgc ttcatcatat ctcccttatc
     3601 tggaccgatg gcccacgata atttagcaga atgttaacaa actaaaatac acaaaactac
     3661 cagaaccccg tggcaaccgg tgcctgatgt tgaccgtcgg aacagtcaac atcgcgatta
     3721 cggtttgaag tccaatggcg cgtttttgaa cacttcatat actgcctggc ttggtggttt
     3781 cgggatcttc gcaagtttag ctgctgccaa cgcagcctga caaagtgcgg gatcgccacc
     3841 ttcaggtttg atatccagta acataccatc gggtgccagt tttatgcgca gcgtacaggt
     3901 tttgcctgca taggacgatg cgtcatagaa cttactttcg atagcagatt taatctgccc
     3961 ggcatagtta ttgatatcgg cccctgatgc gccattgttt ttagtattac cactcccggc
     4021 aggcgaagca ttgttccctt tcgcccctcc ccccgttttc ggtgcattct taccagagct
     4081 tagctcaccg aaaatatcat ctgcctctgc ggcagctttt gctgcagcgg ctttttcagc
     4141 tgctgctttt gccgctgccg cttttttgtc tgcggcggct ttttctgccg ctgcttttgc
     4201 cgctgccgct tttttgtctg cggcggcttt ttctgccgct gctttcttat cagctgctgc
     4261 cttatcagca gccgcttttt ctgctgcttt tttgtcggct gcagctttct ctgctgccgc
     4321 tttctttctt gcttcagctg cagcagcttc tgccgcttcc gctttcttct tcagtgccgc
     4381 agcggctgcc tcggcttttt tctgcgcttc ggctgcggct ttggcggctt ctgcttctgc
     4441 ttttttcttc gcgtctgccg ccgctttctt cgctgcttct tccgcagctt tagcatccgc
     4501 ttcggccttc gctttagcat ctgccgccgc tttcgctgcc gcctcttccg cttgcttctg
     4561 ctttaactcg gcctgttttg cggcttcttc agcctgcttt ttctgttcct gagcagctaa
     4621 ccgctctttc tcaagttgct tcaggcgttc ctgttcagcc gcttgtttct cacgcagttc
     4681 ttcagcagcc tgctgttcct ttatcttgcg ctgctcatca gaacgcttcg cgcttgattc
     4741 ctggctttgc atgcgtttgt actgctcaac taccgcacct gaatcaacca tgacagcgtc
     4801 gatggacgaa ccaccgccgc ctccggctga agcttctata ttctcatcga acgaactcca
     4861 gagcagcgcc gcaaataaga tgacatgcag cactgctgaa ataattatcg cccgcttgag
     4921 cttgtcgttt tgttcggttg cctttgacac tctcggtttc caaaaactgt tcgcctgtta
     4981 cccgctctct ttcaagcaag ggaaacgcag atgtttagat aggctgcgtc attaaaccaa
     5041 ccgatttcac acccgcacta tgtaacaagt tcagtgcttt aattatttca tcgtaaggca
     5101 catcttttgc gccaccgatc agaaagaccg ttttcgggtt ggccttgaaa cggctggaca
     5161 cttccgccac cacctgctct ggtggtaaac gctccaggcg atctttctca accaccacgg
     5221 tgtactgacc aataccagac acttcaacaa tcactggcgg attatcgtta ctgctcaccg
     5281 cctgtgattc agtagcgtct ggcagatcga cctccacgct ctgggtgatg atgggcgctg
     5341 tcgccataaa gatcagcaac agcaccagca gtacgtccag caacggtaca atgttgattt
     5401 cggacttgag atcgcgacga cctcgtccac gcgctctggc catggcttac cccttgttgc
     5461 tctcgctaac ggtaaacgcc tggcggtgca gaatcgcggt aaactcttcc ataaagttgt
     5521 cgtaattcag ttccagtttg tttacgcgct ggttgaggcg gttataagcc attaccgccg
     5581 gaattgcggc gaacagacca attgcagtcg caatcaacgc ttctgcgata ccgggcgcaa
     5641 ccatttgcag tgttgcttgt tttaccgccc cgagggcgat aaaggcgtgc atgatccccc
     5701 agaccgtacc aaacagacca atatacgggc tgatggagcc aaccgtaccg aggaacggaa
     5761 tgtgcgtttc cagattttca agttcacggt tcatggagat acgcatagca cgcgacgccc
     5821 cttccactac ggcttccggc gcatggctat tggcacgatg caggcgcaca aactctttga
     5881 acccgctgta aaatatttgt tccgaaccag tcagattatc ccgtttcccc tgactctctt
     5941 gatagaggcg agagagttcg attccagacc agaatttatc ttcaaacgct tcggcttcgc
     6001 gcgccgctgc gttaagaata cgggtccgct ggataataat ggcccaggat gcgattgaaa
     6061 aaccaatcaa aatcaacatg ataagtttaa ccagaaggct agccttcagg aacaaatcaa
     6121 ggatattcat gtcagtcact gcttaaactc cgcgacaata gacttgggaa gcgcacgagg
     6181 cttcattttg agtgggtcaa cgcaaacaac cagaacctct gcttcattca gcaaagtatt
     6241 ctcggcgttg acaatacgtt gcgtgaaaac caaagaggtg ccacgcattg atgttatttc
     6301 agtctgtatt tcgagcatat cgtcgagccg cgcaggtgcg taatattcca ccgtcatttt
     6361 acgtaccaca aaggcaacgc gttcagccat cagcgcctgc tgactgaagt gatgatgacg
     6421 cagcatctct gtgcgtgctc tttcataaaa agcgacgtaa ctggcgtggt acaccacacc
     6481 accggcatcg gtatcttcat agtagacgcg aaccggccat cgaaacagcg ttgtattcac
     6541 tttacatccc ggtaatgcaa caaaagttag agcttttaaa cgctgctact atacgcgcgc
     6601 gattgaggtt tgggaatgag ggcaagttaa gggagcgtaa aaatttatgg gcctggagcg
     6661 gcccataatt ttttaaagag aattctgatt taaaagaaga agaaaatcag cccgacaatg
     6721 agaacaatat cggcaagcaa tgggcaaaaa atcccttgcc aaagaacctt ctgcggacga
     6781 aagcccacgc cgtgaatcac accagcacag acggcccaca tcagcaataa accatgccag
     6841 atttccagtt cactggtctt cgcggcaaaa cgagatgggt cccaaaacat acatcctgct
     6901 aacagaagcg ccatcacgaa ggaaagcgcc cgcagggggc gcttgtccat taccgcatac
     6961 aaggtcgcga taatcttact catcagatgt cttcttgacc ggctttgcct gattcgacgt
     7021 gttcaagcgc cagcgcggtg attaccccaa acgaacaggc aagaagcgtt cccagaatcc
     7081 atgcgaaata ccacattttt agctccttac ttagtacaga gagtgggtgt tacgttcaat
     7141 atcttctttg gtgatacgac cgaacatttt ccagtaacac caagcggtgt agagcagaat
     7201 gatcggtacc agaaccaccg caacccaggt catgacgtta agcgtcagct ggctggaagt
     7261 tgcatcccac attgtcagac ttgcgttcat catggtgctg gacggcatca caaacgggaa
     7321 cattgcgata ccggctgtca ggatgatgca ggccagcgtc agggaggaga acacaaacgc
     7381 ccacgcggct ttatccatac gtgcagtcag gatggtcagc agcggaagaa ccacacccag
     7441 tgccggaata gcccacagaa ttggcgtgtt gttgaagtta accagccatg cgccagcttc
     7501 acgaaccact tctttattca gtgggttaga ggctgcgtaa tggtccattg tcgatttcac
     7561 gacataacca tcgataccgt acatcaccca tacgccagcc agtgcgaaac agaccagtgt
     7621 caccagcgca gccacctgag ccgttgcacg ggtacgcagg tgcagttcgc ccacggtacg
     7681 catttgcaga taggttgcgc cctgagtaat gatcatccct acgctcacca cgcctgccag
     7741 caggccgaac gggttaagca actggaagaa gttaccggtg tagtacagac gcagatattc
     7801 atcaacgttg aacggtacgc cctgcaacag gttaccgaac gctacaccaa ttaccagcgg
     7861 cggaacgaaa ctaccaatga agatgcccca gtcccacatg ttacgccagc gggtttcttc
     7921 aatcttggag cggtagtcaa aaccgaccgg acggaagaac aaagacgcca gcacgaggat
     7981 catcgccaca tagaagccgg agaacgcagc ggcatagacc atcggccagg cagcaaagag
     8041 tgcgccgccc gcggtgatca gccaaacctg gttaccgtcc cagtgtggtg caatggagtt
     8101 aatcataatt cgacgctcgg tgtcgttacg accgaggaaa cgggtgagca tgcccacccc
     8161 catgtcgaaa ccgtcagtga ctgcaaaacc aatcagcaga acgccaacca gcagccacca
     8221 gataaaacgc aatacttcat aatcgatcat ttgacgactc ctgtcttagc gtgccggctg
     8281 agtagtcgtg gaagactgct caaagtgata gcgaccggtt ttcaggctgc ttgggccgag
     8341 gcgtgcaaac ttgaacatta agaacaattc tgccaccagg aacagggtat acaggccgca
     8401 aatcagcacc attgagaaga tgagatcgcc tgcggtcagt gacgagttcg ccacagctgt
     8461 cggcagcact tcaccgatag cccacggttg gcggccatat tcagccacga accagcccgc
     8521 ttctacagca atccacggca gcggaatacc gtacagcgcg gcgcgcagaa gccatttttt
     8581 ctcgccaatg cggttgcgga tgacactcca gaaagagagc gcgatgattg ccagaagcag
     8641 gaagccacac gccaccatga tacggaacgc aaagtacagc ggcgctacac gcgggatgga
     8701 gtctttggtt gcctgttgaa tctgcgcttc agtcgcatca gccacgtttg gcgtatagcg
     8761 tttcagcagc agaccgtaac cgaggtcttt cttcatgcta ttgaactggt cacgaaccgc
     8821 ctggtcggta gaaccagaac gcagttgttc gagcagagag tacgccttca tcccgttacg
     8881 aatgcgttct tcatgctgca ccatcagctc tttcaggccg ataaccgggg tatccacgga
     8941 acgcgttgca atgatgccca gtgcgtaagg gatctgaatc gcaaatttgt tcgtctcctc
     9001 ttcctgatca ggaatgccga acagagtaaa ggcagcaggc gcaggttgcg tttcccactc
     9061 ggcttcaata gcagccagtt tggttttctg cacgtcgccc atttcgtagc cggattcatc
     9121 acccagaaca ataacagaca gaacagcagc cataccgaag ctggcagcga tagcaaagga
     9181 gcgtttagcg aaggcgaagt cacgaccttt cagcatatac catgcgctga taccgaggat
     9241 gaacatcgcg ccagtcacat aaccagacgc tacagtgtga acgaatttca cctgagcaac
     9301 cgggttaagc accagctcgg agaagctcac catctccata cgcatagttt caaagttgaa
     9361 atcggacgcg attgggtttt gcatccagcc gttcgcaacc agaatccaca gtgcggacag
     9421 gtttgaaccg agcgccacca gccaggtgac acacatatgc tgaactttac ccagacgatc
     9481 ccaaccgaag aagaacagac ctacaaaggt ggattcgagg aagaaggcca tcagaccttc
     9541 gattgccagc ggcgcaccga agatatcccc tacatagtgg gaatagtaag accagttagt
     9601 cccgaactgg aactccatgg tcagaccggt agccacaccc agagcgaagt tgataccaaa
     9661 caacttgccc cagaacttgg tcatatcttt ataaatctgt ttgccggaga ggacgtagac
     9721 cgtttccata atggccagca ggaacgccat accgagcgtc agtggcacaa aaaggaagtg
     9781 gtacatcgcg gtcaaggcaa actgtaagcg cgacagttcg actatatcta acatcatgac
     9841 tccttgctca tcgcatgaag actccgagag tgaaccccgt tagaaagggt cacacgcatg
     9901 ccccaataga aatttgtttg aacactttca tcataccatg tgaatgcaca caaaaacggc
     9961 gtaaagaaag gttgcaaaca tgttaataaa aactcaaatt gatcccacgt atatattacg
    10021 ccgcaaaatc cttacaataa acaggttttt gttgaagcaa atttgcattt ttcgacggtg
    10081 atcaatttat agctaaatta ccgcctttca gccaatttga tcgagaacaa tttatctctt
    10141 tttgatgc
//
LOCUS       DAZPDU010000157         9809 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZPDU010000157 DAZPDU010000000
VERSION     DAZPDU010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9809
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.159"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..347
                     /locus_tag="ACHURS_003512"
     CDS             <1..347
                     /locus_tag="ACHURS_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFR1239151.1"
                     /translation="PWFLKIVHSENQSKGVHYAKSLRLLEINHAHLQLMESLLDEGKK
                     HNIFKPDIDPLQVNINIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILS
                     WLLVDPSSTAHE"
     gene            complement(331..969)
                     /gene="ytfB"
                     /locus_tag="ACHURS_003513"
     CDS             complement(331..969)
                     /gene="ytfB"
                     /locus_tag="ACHURS_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFR1239152.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            1188..1808
                     /gene="fklB"
                     /locus_tag="ACHURS_003514"
     CDS             1188..1808
                     /gene="fklB"
                     /locus_tag="ACHURS_003514"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR1239153.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            2117..3529
                     /gene="cycA"
                     /locus_tag="ACHURS_003515"
                     /gene_synonym="dagA"
     CDS             2117..3529
                     /gene="cycA"
                     /locus_tag="ACHURS_003515"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFR1239154.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(3574..4236)
                     /gene="ytfE"
                     /locus_tag="ACHURS_003516"
     CDS             complement(3574..4236)
                     /gene="ytfE"
                     /locus_tag="ACHURS_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFR1239155.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(4344..5309)
                     /locus_tag="ACHURS_003517"
     CDS             complement(4344..5309)
                     /locus_tag="ACHURS_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR1239156.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(5417..6277)
                     /gene="qorB"
                     /locus_tag="ACHURS_003518"
     CDS             complement(5417..6277)
                     /gene="qorB"
                     /locus_tag="ACHURS_003518"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFR1239157.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            6366..6746
                     /locus_tag="ACHURS_003519"
     CDS             6366..6746
                     /locus_tag="ACHURS_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFR1239158.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(6864..8807)
                     /gene="cpdB"
                     /locus_tag="ACHURS_003520"
     CDS             complement(6864..8807)
                     /gene="cpdB"
                     /locus_tag="ACHURS_003520"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFR1239159.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKTGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINTNAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSNKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            8997..9737
                     /gene="cysQ"
                     /locus_tag="ACHURS_003521"
     CDS             8997..9737
                     /gene="cysQ"
                     /locus_tag="ACHURS_003521"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFR1239160.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
BASE COUNT         2265 a         2484 c         2551 g         2509 t
ORIGIN      
        1 acccttggtt tttaaaaatt gtgcatagtg aaaatcaaag taaaggtgtg cattatgcaa
       61 aatcactgcg tctcttagaa attaaccatg cgcacttgca gttaatggaa tccttattgg
      121 atgaagggaa gaaacacaat atttttaagc cagatatcga cccattgcag gtgaatatta
      181 atattgctgc gcttggggga tattatttga tcaaccagca tacgcttggc ctggtttatc
      241 acatcagtat ggtttcaccc caggcactgg aagccagacg taaggtcatc aaagagacaa
      301 tccttagctg gcttttggtt gacccttcat ctaccgcgca cgaataaaac tgccatccgg
      361 ctggcgggta aacaggacct gttgattatt ccccgtatca atggttaagc ccgtcaccac
      421 gccgctggcg ttctggcgga ttttcaccat ctgcccattt tgcaaattac tcagcggctt
      481 acccgcgcct tctacctgcg ccattgcata gacatcggta gcgggcagtc cgtggtcgcg
      541 gaacagctgc gccatcgttt taccgggttc gacacggtaa gagcgccatt gattatcaat
      601 tccgctatct ggctggaatg gttgcgtttg ggtagtttgc ggctgttctt ctggctggcc
      661 ttcctgtata ggttctggag cgaccggagc cacctgatca ggatcatttt gtggtgtgac
      721 taattgcgcc cgcagctgtt cttctgtcgg cggttgtgat tgcgactgaa tatctaactg
      781 cgcttcacgc gtcaccaccg gcgcgttggg cgtatcatca gatggaagca gaaatccgac
      841 caccaataca atggcggcaa taatgatccc ccgacgatgc atcggcggca gcgggtccat
      901 gaagcgaaaa ttgtccggcg cgtgccagac tttctccagg gttggtttta gttcaaagcg
      961 cccgggcatg gtttcctcct gctccgcgtc ttgttcctca atcatagcct atgaataagc
     1021 taacgctatg atgtccgtgg taaacccgcc tttattatat tcatacggga tattgctatt
     1081 gtttcttttt ccccgggatt tgtcatcatt cctgcgacaa agttttaccc aaagaagtgt
     1141 ggctgatatg ctgcccgcta cttttatacc ctaagaaagg aaatacgatg accaccccaa
     1201 cttttgacac catcgaagcg caagcaagct acggcattgg tttgcaggta gggcaacaac
     1261 tgagtgaatc tggcctggaa gggctgctgc cagaagcact ggttgcaggt attgccgatg
     1321 cgctggaagg caaacatccg gctgtgccgg ttgatgtggt gcatcgcgcg ctgcgtgaaa
     1381 tccacgaacg cgccgatgcc gttcgtcgtc agcgtttcca ggcgatggct gctgaaggtg
     1441 tgaaatacct ggaagaaaac gccaaaaaag aaggtgtgaa tagcaccgaa tctggcctgc
     1501 aattccgcgt gatcaaccag ggtgaaggcg caattccggc acgtaccgac cgcgttcgtg
     1561 ttcattacac cggtaaactg atcgacggca ccgtgtttga cagctccgtt gctcgtggtg
     1621 aacccgctga attcccggtt aatggcgtga tccctggctg gattgaagca ctgactctga
     1681 tgccggtagg ttctaaatgg gaactgacta tcccgcagga actggcatat ggcgagcgcg
     1741 gcgcaggcgc atccatccct ccgttcagca ccctggtgtt tgaagtcgaa ctgctggaaa
     1801 tcctctaagc agcgcattct gttcccctcg aaccagaggg gagcaggcat tcagcaataa
     1861 acccttcagt ttgccaaacg gcgctatttt gtgttgcaaa gaccccgtaa gcgtgtattt
     1921 ttgtgagctg tttcgcgtta tcaccgtgat atgacactca ctttaaacat aaaattaaca
     1981 ttagatctaa atcttagtat tcatcccgcg tattgttacc taatatcgat gagtcccgat
     2041 acagattcgt cgtatcatag actgactaaa ggccgtagag cctgaacaac acagacaggt
     2101 acaggaagaa aaaaacatgg tagatcaggt aaaagtcgtt gccgatgatc aggctccggc
     2161 tgaacagtcg ctacggcgca atctcacaaa ccgacatatt cagcttattg ccattggcgg
     2221 tgccattggt acggggttgt ttatggggtc cggcaaaacg attagccttg ccgggccgtc
     2281 gatcattttc gtttatatga tcatcggttt tatgctcttt ttcgtgatgc gggcaatggg
     2341 ggaattgctg ctttcgaatc tggaatacaa atcttttagt gacttcgctt ccgatttact
     2401 cgggccgtgg gcaggatatt tcaccggctg gacttactgg ttctgctggg ttgtaaccgg
     2461 tatggcagac gtggtggcga tcactgctta tgctcagttc tggttccccg atctctccga
     2521 ctgggtcgcc tcgctggcgg tgatagtgct gctgctgacg ctcaatctcg ccaccgtgaa
     2581 aatgttcggt gagatggagt tctggtttgc gatgatcaaa atcgtcgcca ttgtgtcgct
     2641 gattgtcgtc ggcctggtta tggtggcgat gcactttcag tcaccgactg gtgtggaagc
     2701 gtcattcgcg catttgtgga atgatggcgg ctggttcccg aaaggtttaa gtggcttctt
     2761 tgccggattc cagatagcgg ttttcgcttt cgtagggatt gagctggtag gtacaaccgc
     2821 tgcggaaacc aaagatccgg agaaatcact gccacgcgcg attaactcca ttccgatccg
     2881 tatcattatg ttctacgtct tcgcgctgat tgtgattatg tccgtgacgc cgtggagttc
     2941 ggtagtcccg gagaagagcc cgtttgttga actgtttgtg ttggtaggtt tgcctgcggc
     3001 tgccagcgtg atcaactttg tggtgctgac ctctgcggcg tcttccgcta acagcggcgt
     3061 cttctctacc agccgtatgc tgtttggtct ggcgcaggaa ggtgtggcac cgaaagcgtt
     3121 cgctaaactt tctaagcgcg cagtacccgc gaaagggttg accttctcgt gtatctgtct
     3181 gctcggcggc gtggtgatgt tgtatgtgaa tcctagcgtg attggcgcgt tcacgatgat
     3241 tacaaccgtt tccgcgattc tgtttatgtt cgtctggaca attatccttt gctcgtacct
     3301 ggtttaccgc aaacagcgtc ctcatctgca tgagaagtcg atctacaaga tgccgctcgg
     3361 caagctgatg tgctgggtat gtatggcgtt ctttgtgttc gtggtcgtgt tgctgacact
     3421 ggaagatgac actcgccagg cgctgttggt caccccgctg tggtttatcg cactggggct
     3481 gggctggttg tttattggta agaagcgggc tgctgaactg cggaaataac cgcattatca
     3541 tgctggatgg cgcaatgcca tccagctttt agattactca cccgccagcg cgcgtgggaa
     3601 cagtacattg ttttccaggc tgatgtgatc catcaggtca tcaatcagtt cattaatacc
     3661 gttatacatc gctttccagg tggtgcaggc ttccggcggc ggtgtgacgt tattggtggt
     3721 gtgtttaatc acttccagca gttcgcccgc ttcatcgtgc tcgctttcca tcacgctgat
     3781 tggtcccatt gcctggctgc ccatgccttg tttgatcatc gggaagagga tctgctcttc
     3841 tttcatcatg tggctggata gctcttcatg cagcatagtc aggtattttg tcagcccttt
     3901 tggcacgctc ggtttgtcgg cgtgaacgcg ctcgacttta gtcgcttgca ggatcagctc
     3961 cggcagttgc tcgcggtgac gatcgtggta gcgcacgatg atatggtcaa tgatttctgc
     4021 cagcggggcg ctacgccagt ctttctcaat cggttgttca gcaagctttg ccagttcagc
     4081 ttcaatgacc tcaacatcca gttctttacg tgccgccgcg cgcgccagcg tttgcttacc
     4141 gccacagcag taatccatat catatttacg aaacagagct gaagcgcgag gaatagagag
     4201 cgccagttca cctaaaggtt ggtcgcgata agccatagct gatacctcat tcttaataat
     4261 ataagatgta ttttaaatgc atctttaagg caaaagctat aacccttacg tagcagaggc
     4321 ttatttaact cactgccagc gtgtcaactt tctgagacag attcagttaa aggtttttct
     4381 ggttttgctc tgaccgcaat gaccacgcca atcactaaca gcgcgatacc gctcagcgtc
     4441 attagcggcg gcatttgctg gcggagtaaa aaggtgtaca gcaaacctgc cagcgtttcg
     4501 aaaacaatca gcggcccgag aatcactgtc ggtaatcgct ggctggcgac gttccagcag
     4561 agtgcgccaa cccatgagca aagcacggct atcgcaacca tcagactaat aaacaccagc
     4621 ggacgggggc caaaaggtaa ggagaagtcc ggcgtttgta tattcagcca gtaacaggcg
     4681 acgagatagc cgatgagaga aaccggcagt gtgaccagcg cctgcgccgt tgcccacatc
     4741 atcggatgtt tgtcgggatt ttcccgcagc cagcgggcgt tgcgcagggc ataccacgcc
     4801 cagcagacca cggaaactaa cgccagcacg atgccagagg tataacgtgc ccagtcaaaa
     4861 tcggggagtc cgtggtttaa ctcagcaata ttcacacacg ccaggccgat gccaatacaa
     4921 accagtgccg gggcgagttt tccccatgcg agtttgccgt cgcgctggct ataaagcaga
     4981 ttggcaaaaa cgggaatcac caccggcagg gtgccgataa tcatcgtgga aacaggcgcg
     5041 ccagtacgtt gaatggcact ggcaaggcag aagtaataga taaggttgcc catcatggtg
     5101 agcatcaagg cggtaagcca gtcgcgacgc gccaactgac gcagacgcac gcgtcccagc
     5161 caggcaatgg gcagcgcaat taaccctaac gccagataac gccccatcga ctgcaacatc
     5221 gccgggtatt ccggtacgat caacggcccg acaaaaataa gcccccacat caaccctgct
     5281 aacagggcgt acagcacgcc gctaatcatt actggcatcc attggtctgt cagaagaaag
     5341 ttcaggaagc agacatgatt gctcccggca tcccgtcatt atcagagagg atgcgggagg
     5401 atgccacttt aattaactag ttattaacat taaaaagatg gcttacgctt tcggctaacg
     5461 ttgtcgttgg gcggccaatc aatttgctaa gcgttttgct gttatcaaac agaccacctt
     5521 tcgatgcgcc aacgtcagaa tccgccagca tatccgccag tccgtcgggc agtccgacgc
     5581 ttttcagtgc cgcggcgaaa tcggcttcgc tcagattttg ataggtaacc tgtttgccgc
     5641 tctgtttggt cagctctgcc gctaactgtg tcaacgtcca ggcactatcg cccgccagtt
     5701 cgtaaacctt gccttcgtga ccggcttcgc taatcacgcg tgccgcagct gccgcataat
     5761 ctgcccgcgt tgctgaggcg attttgccat cgcccgccgc accgataaat acgccgtgtt
     5821 ccagtgctgc cggggcgctg gcgaggtagt tttcgctgta ccagccgttg cgcagcaggg
     5881 tgtaaacgat gccagaatca gccagcattt tctccgtctc aatgtgctca tcggcgaggc
     5941 cgagcgggga ggtatctgca tgtagcaggc tggtataagc gataaatttc acgccagccg
     6001 cctttgcggc attaataaca ttacgatgct gcggggcacg ttgacccact tcgctggaag
     6061 agatcagcag tagtttttcc actccctgaa gtgcagatgt cagtgcggct tcatcgccgt
     6121 agtcagcctg acgcacggta atgccttgtg ctgctagagc ctgggctttt gccggattac
     6181 gaacgatagc cactatttgg ctggcaggaa ccgttttcat caaggattca ataatatagt
     6241 gaccaagttg gccagtggca ccagtaatag cgatcatggg aagtctccat cgttttgtct
     6301 tgtgtttaaa tacgctaaca cctaaactta cttttagtaa gtacgtacaa aaaggtaagt
     6361 atgaaatgag tcaggttagc ctgtcgcaac aactgaaaga gggtaatctc tttgcggaac
     6421 agtgcccgtc gcgcgaggtg ttgaaacacg tcaccagccg ttggggggtg ttgattctgg
     6481 tggcgctacg cgaaggtact catcgcttta gcgacctgcg gcgcaaaatc ggcggggtga
     6541 gcgaaaagat gcttgcgcag tcgttacagg cgttagaaca ggatggtttt cttaaccgta
     6601 tcgcgtatcc ggtggtgcca ccgcatgttg aatacagcct cacgccgctg ggcgagcagg
     6661 tgagcgaaaa ggttgctgca ctggcggact ggattgagtt gaatttgccc gaggtgttgg
     6721 cggtgcggga tgaacgtgcg gcataactgc cggatgcgcg gcctacggtg tatgacttgt
     6781 agggcggata agatgcgcca gcatcgcatc cgccaataaa tgccggatgc gattgatgat
     6841 ttataggccg aaaaagaagt gttttactta ctcaaatcca cctgataaat cgcaaacccg
     6901 atatcatcgg tcgcgacttt attcatcgga tactgccctt tctctttaat aaacgctgcg
     6961 gctttatttg acggggaagt ttcgaaacgg atatccagtt tcttatcggc agctatcggt
     7021 gctaaacgcc agttgttatc tgccgccggg tgaatttccc ccgcacgttt cgactcatca
     7081 gcaatccacg ctgccagcac cgagcggttc tcatccggtg aagcaaaagc gatatggctg
     7141 tcgcccgtac cggcaaattt gccgccgtaa gcgcgatagt tattggtggc aacgaggaac
     7201 atggcgttcg gatcaatcgg cttgccatta aaggtcaggt tcttaatcct ttccgcattg
     7261 gtattaatca tctggcactc gccgtcataa cgggcgggct gggtaacatc aatctgataa
     7321 ttcacaccat caataacatc aaagttataa gtgcggaaac catcccagtt gatgagtgac
     7381 tgtggtttcg tgctgttagg atcaatctgg ttaaactgtc cggcggagca ctccagccac
     7441 tctttcacct ctttaccgct ggctttcact acaatcagcg tattggggta gagataaaga
     7501 tcggcggcat tacggaaggt caactggcct ttttccacct ccacatagct tgccgggtca
     7561 tttttgcgac caccaacttt aaacggtgcg gcagctgaaa gcaccggcag ttttgccaga
     7621 tccggatcgc cctgaatgta atgttcgaca tacgcttttt gcgcgttgtt cacgacttgc
     7681 acggtcggat cgtcctgcac cagcgccaga tagctataca tattgtcggc agatttaccg
     7741 attggcttgc tgacgaactg gcgtgtggca tcgtgatcgg ctttgagtgt ttctaccagc
     7801 ttgctgtctt ccgccgcgag ggatttttta ttggcgatgt cgtaaatcgg ccgagcttcc
     7861 gctttcgcct gcgtcacctg ccatttaccg ctgtcattac tgagttgtaa gtcgaccacc
     7921 ccaagatgat cgccccacat gcccggcatt accgccggaa caccattcag cgtgcctttg
     7981 gtgatatcag ccccttcgat atcagcaaaa tctttacccg ggaaaacggc gtgagcatga
     8041 ccaaacataa tggcgttaac gcccggaatt tcactgaggt aataaactga gttttccgcc
     8101 atcactttat acggatcggc agacagcccg gaatgtgcca gaacgacaac gacatcggca
     8161 cctttctcgc gcatttcagg cacgtatttg cgcacggttt cggtaatatc attcaccgtc
     8221 actttcccgg ataaattagc tttatcccag cccatgattt gcggcggcac gacgccaata
     8281 tagccaatct tcagcgtctg tttttttccg tctttatcga ccacttcggt atctttaatt
     8341 aaatacggtg taaacattgg ctgtttggtt ctggcgtcaa tgacgttggc atttacataa
     8401 gggaatttcg ctcccgccag cgcatttttc aggtaatcca gaccgtagtt aaattcatga
     8461 ttgccgagtg taccgaccgt ataatccagc gtattcagcg ccttataaac cggatgaaca
     8521 tcacctgttt ttaatccttt cgccgacatg taatctgcca gcggactccc ctgaatcaaa
     8581 tcgccgttat cgaccagtac gctgtttttc acttcattgc gggcatcgtt aatcaggctt
     8641 gccgtacgta ccagtccgaa tttttccgtg gcggtgtctt tgtaataatc gaaatccatc
     8701 atgttgctat gcagatcagt ggtttccatg atacgcagat cgaccgtcgc tgcattcaca
     8761 ctggcggcaa tcagcgtggc caggagcgtt gcgctaaact taatcatcag ggacatcctt
     8821 ttatcatcgg gaatacgaaa gaaaagggag aataaacgtc ttacttatag aacagtgaag
     8881 aatgccacaa ttttacgctt tgaaaatgat gacactatca cagttggcgc attcattaac
     8941 gatagggtat aagtaaaaca ataagttaac accgctcaca gagacgaggt ggagaaatgt
     9001 tagatcaagt atgccagctt gcacggaatg caggcgatgc cattatgcag gtctacgacg
     9061 ggacgaaacc gatggacgtc gtcagcaaag cggacaattc tccggtaacg gcagcggata
     9121 ttgccgcaca caccgttatc atggacggtt tacgtacgct gacaccggat attccggtcc
     9181 tttctgaaga agatcctccc ggttgggaag tccgtcagca ctggcagcgt tactggctgg
     9241 tagacccgct ggatggtact aaagagttta ttaaacgtaa tggcgaattc accgttaaca
     9301 ttgcgctcat tgaccatggc aaaccgattt taggtgtggt gtatgcgccg gtaatgaacg
     9361 taatgtacag cgcggcagaa ggcaaggcct ggaaagaaga gtgcggtgtg cgcaagcaga
     9421 ttcaggtccg cgatgcgcgc ccgccgctgg tggttatcag ccgttcccat gcagatgcgg
     9481 agctgaaaga gtatctgcaa cagcttggcg aacatcagac cacgtccatc ggctcttcgc
     9541 tgaaattctg cctggtggcg gaaggacagg cgcagctgta cccgcgcttc ggaccaacga
     9601 atatttggga caccgccgct ggacatgctg tggctgcagc tgccggagcg cacgttcacg
     9661 actggcaggg taaaccgctg gattacactc cgcgcgagtc gttcctgaat ccggggttca
     9721 gagtgtctat ttactaaatt cagatggcag aaacagtgta tttcctgatt ctgccatcct
     9781 gatttctccc aacctaaaaa gttataaat
//
LOCUS       DAZPDU010000158         9707 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZPDU010000158 DAZPDU010000000
VERSION     DAZPDU010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9707
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.160"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..146)
                     /locus_tag="ACHURS_003522"
     CDS             complement(<1..146)
                     /locus_tag="ACHURS_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239161.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVREITKMMNGKLIYSWLKWQCG
                     EFFSL"
     gene            224..1318
                     /gene="ddlA"
                     /locus_tag="ACHURS_003523"
     CDS             224..1318
                     /gene="ddlA"
                     /locus_tag="ACHURS_003523"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFR1239162.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1342..1554)
                     /locus_tag="ACHURS_003524"
     CDS             complement(1342..1554)
                     /locus_tag="ACHURS_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFR1239163.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1814..2122
                     /locus_tag="ACHURS_003525"
     CDS             1814..2122
                     /locus_tag="ACHURS_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFR1239164.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2181..3275)
                     /gene="yaiW"
                     /locus_tag="ACHURS_003526"
     CDS             complement(2181..3275)
                     /gene="yaiW"
                     /locus_tag="ACHURS_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFR1239165.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLAITFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3288..4508)
                     /gene="sbmA"
                     /locus_tag="ACHURS_003527"
     CDS             complement(3288..4508)
                     /gene="sbmA"
                     /locus_tag="ACHURS_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFR1239166.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGWLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4860..6017
                     /gene="ampH"
                     /locus_tag="ACHURS_003528"
     CDS             4860..6017
                     /gene="ampH"
                     /locus_tag="ACHURS_003528"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFR1239167.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6018..6641)
                     /gene="iprA"
                     /locus_tag="ACHURS_003529"
     CDS             complement(6018..6641)
                     /gene="iprA"
                     /locus_tag="ACHURS_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFR1239168.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6729..9617)
                     /locus_tag="ACHURS_003530"
     CDS             complement(6729..9617)
                     /locus_tag="ACHURS_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR1239169.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATYNTFGYHDDAATIYN
                     WGDSTKIDHDYLTYGGYVYDHAADGYFDTTFSGDTVNGVISTYYLNHDYGTDTGNTLN
                     ISNSIVHGMVTSYQVGYGDYVWTNGSDYTGHDWVDGDIFTLNIANSTIDDDFDAFYFT
                     DTYLDADGKTSKTDYDRLVTAGLGTAVTLDVESNINISNNSHVAGITLVQHDLGNATY
                     NTEGHQWDNNIVVNDSTVTSGSWTEDEQSDRGHFGNSVEPSDYGNGASPADDVALAFI
                     DDDTSDYRMVNNVTFNNSQLLGDVVFDSTWNANFDATGHLIDNSTTAYTHGGWATDDQ
                     NVDHLNLTLNNTKWVGSANIDYDVVVADEAFYDVAPNSLNPYASYSEDGWNRVDNANA
                     FQSGVFDVVLNNGSDWETTKTSLIDTLAINSGSQVNVSADSSLTSDTITLNGGSSMEV
                     NGEVNTDHLTIDTFSTVNFGEDTASAWAYAPLYANTITVTNGGVLDVNTNMNDISSVF
                     ATDTLELTSGNVKDNNGNVYAGVFDIHSNDYILNADLVNDRTNDTSKANYGYGVIAMN
                     SDGHLTVNGNNDINNGDEVDNSSADNVVAATGNNKVRIDNSTGAGAIADYAGKQLIYI
                     DDTKTNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSANTNIWNL
                     EQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKVDGN
                     NAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDL
                     SATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPYGSVSGLFQSGDDYQLSND
                     MKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNG
                     TEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
BASE COUNT         2493 a         2403 c         2340 g         2471 t
ORIGIN      
        1 agagaaaaaa actcaccgca ttgccattta agccaggaat aaattagctt cccgttcatc
       61 atttttgtga tctcgcgcac atctttgaga taacgcactt tcttcaccag tacgttgcac
      121 gctttgtcag cagaagattg caacaatctt tcagaaggcc gcgaatcccc cgttttgtcg
      181 tggtaatgta tgcactttgt gttagacggg ataggttttt aagatggaaa aactgcgggt
      241 aggaatcgtt tttggtggta aatcagcgga acatgaagtg tctctgcaat cggcaaaaaa
      301 cattgtcgat gccattgata aaagtcgctt cgacgttgtg ctgctgggca ttgataaaca
      361 agggcaatgg cacgtcagcg atgccagcaa ttatctgcta aatgcagacg atcctgccca
      421 tattgcgttg cgcccttcgg cgaccagcct tgcgcaggtg ccaggtaaac atgagcatca
      481 gcttatcgac gcgcaaaacg gtcagccgtt gccgacggtg gatgtcattt tcccgattgt
      541 ccacggtacg ctgggcgaag atggttcctt gcagggaatg ctgcgggtcg ccaatttacc
      601 gtttgtaggt tctgatgttc tggcttcagc agcctgtatg gataaagatg tcaccaaacg
      661 tctgctacgc gatgccgggc tgaacattgc gccatttatt accctgacgc gcgctaatcg
      721 tcacaacatc agttttgccg aagtggagtc taaactgggg ttaccactgt ttgtaaaacc
      781 ggctaatcag ggctcttctg ttggtgtcag caaagtaacc agtgaagaac agtacgcaat
      841 tgccgtcgat ctggcgttcg agttcgatca taaagtgatc gttgagcaag ggatcaaagg
      901 tcgtgagatc gaatgcgcag ttctgggcaa cgacaatccg caagccagca cctgtggcga
      961 gatcgtactc accagcgatt tctatgccta cgacaccaag tacattgacg aagatggcgc
     1021 gaaagtggta gttccggcag ccattgcacc agagatcaac gataagatcc gggcgattgc
     1081 cgttcaggct tatcagacgt tgggatgcgc aggcatggcg cgtgtagacg tgtttttaac
     1141 cccagagaac gaagtggtga tcaacgagat caacacactg cctggcttca ccaacatcag
     1201 tatgtatccg aagctgtggc aagccagcgg tctgggttac accgatctga tcacacgttt
     1261 gattgagctg gcgctggagc gtcacgctgc ggataacgca ttgaaaacca caatgtaata
     1321 ttttaacgcc cgataatatt cttattgggc gttttcagtt tcttcatcgc gacgacgaat
     1381 aatcaacccc gccagccaga aactaatcac ccacgtcacc agaccgacgg cataggtctg
     1441 ccaacctttt gcttcaaatc ccagtaaccc caccacgcca ttaagaatga atataaggcc
     1501 aatggcgaac gcatagtagt gccagtcacg gcggattttt acaggcagat tcatggcagc
     1561 tccgttaacg aaaaacaaca tccttgcata ttctgtaaca accgtctgtg gtgtagacaa
     1621 cattttcgta attgttaata aatttaactt aaggttgcca ttttgtaact cacaacgaag
     1681 attcacgctt cgggaatatt cccaacatca atttaccatg attctgatat tgacaatcta
     1741 aatgacctgt cagacttgcc ataagccgct ggtatcacac cagaagagaa gcagatgact
     1801 tccggaggtt tttatggctg atttcaccct gtcaaaatcg ctgtttagcg gaaaatatcg
     1861 caatgcctct tcaacgcctg gcaacattgc ctatgcgttg tttgtgctgt tttgcttttg
     1921 ggctggggcg caattgctga acctgttagt gcatgcgccc ggcgtctatg agcgtttaat
     1981 gcaggtccag gaaacaggtc gcccacgggt ggaaattggt ttaggtgtcg gcaccatttt
     2041 cgggctgatc ccgtttttag taggctgcct catttttgca gtggtggcgc tatggctgca
     2101 ctggcgacat cgccgccagt aagatttacg ttgccagata cggcctaaaa cgccttatct
     2161 ggcatgacat tcctgtttga tcactgtttc atacaacgcg cccgccgttc gtctacgcgc
     2221 ttcgcaaacc aggccgtagt caggttgcgg gtgattttcg ggctttccag ttgaatgcca
     2281 ggcaacattt cgcggggtag tgatttaccc gttttcgctt cggcaagttg ataaactttc
     2341 ttgtacagcg ccgtctcctc aaaagagaag ctgtcacctt tctctaactg acggcgaatt
     2401 tcgctgtcgc tcatccccag ttttcccgcc agtttgcgcg ttgccagttc ggttttcccg
     2461 ggttctttac tgtcgtaacg aattaaatca ccgtccagtg ccagcttcac gccgctggcc
     2521 ttactgactg cgttctgaaa cgcggcatta cggctggcgt accagccagc gttaaaatca
     2581 gcaaagcggt atatcggtgc gctatagctg gcgggataat tcagtaaatg gtaagtacca
     2641 aaccacaacc cgccgcggcg actgaagact tcctgacgaa ctgtaccgtc cattttccac
     2701 ggataccctt tggtatgctg ttcggcaaaa gcaatgctga cctgcatcgg cccaccggtg
     2761 cgcaccggat taagcgaacc aaacagcgtc tgccccattg gtaccatgct gatcagatcg
     2821 tcaaaaatcg cgctcaattg cttttctgta cgcaccgaat ctaaccgttc gctataactt
     2881 ttgccgtttg gcgatttgat ttttagcgcg gtatgaacca gaaaagcagg aatgtgcatc
     2941 cgttcagcac gacggtcaat ctcttgccag gcgatcttgc ttaagcccgg aacggccgga
     3001 tctgcctgat aatttgactc ctgctgcgcc accgccagca ccgagcagac gttttccagc
     3061 gtcggtgcca gcccctgact ttcaaaggtg atggcgagat ctttcgccca ggcatcgcgg
     3121 tctttcacgc tcgcaggcat cttctgccgc acgacgctcg ctacatctat cgctttttcg
     3181 ccctttttca acggttgcgg agcctgacta ctacaccccg ctaaaaccag aaccgccaac
     3241 aatgacagcg aagatagatg attgacgcgc gacattactt ctccttttta gctcaaggta
     3301 tgggttactt cctgaatttt gtcaccatcc agctcatgtt caaagctgcg cagacgtttg
     3361 tagatagaca tcaactcaac cagtgtggtc catgagttaa tcaggtactg gaaagcaccg
     3421 cgaacctgac caaaaacgtt ggtaatctgc gtcatcaggc cgagcgtaat cgtaccggca
     3481 acaattgacg gaaacagcaa gaacaaaccg aaaacgttat cgacctgcaa atagagaatg
     3541 cgggcgatgt tgaaatacat atagtgaaaa tagaggcgaa aatagttttt ccgtacggcg
     3601 ctaaacagct cgcgtaccgt aggcggcgtc gcgcgcgtgg catcgtcttc accataaacc
     3661 agctctttac ggtaggcggc ctctactcgc tggtttttaa actccagccc cggcagtttg
     3721 atccctacca ctgccagcca tccggtcccc atcagcgacc agacgatagc ggcaatcacc
     3781 agaccatacg gaatgtgccc gataatcggc agctccggca catgcgcgga gagcgttacc
     3841 agcaccggca ggaaggcgat caacgtcatg atggcgttga taaaactgac gcccatattc
     3901 tccagcgttg aagcaaaacg catggtgtct tcctgcacac gctgtgcggc accttcgata
     3961 tgacgcagtt gttgccagtt cgccatgtaa tattcgttca tcgccgtacg ccagcggaac
     4021 acatagtgac tgacaaagaa gttgttcagt acactgatca ccaccgcgat cagcgcaatc
     4081 cccagaaaga cgcccacttc gcggtaaaat tgttcgatag tgactttatg cggcgaactt
     4141 agcgcggttt gaatcaaatc atagaacggt gcataccagg cgttgacggc gaccccgact
     4201 tccaccaaaa accaggtgac gaagatgatc agtgcagtac cgagtattga ccagtattgc
     4261 caacggtgcg ggctgtagat aaaccagaaa aatgcaaaaa gtcctacgca aacaatgtag
     4321 taagcgtaaa aaatcaggaa atccaacgac cagaaacgcg cggcgctaat cgggatctgc
     4381 ccggaagcgc cggtgatacg cgccacccag tctcccccac cggcttgcca gaagataacg
     4441 gcaatcaatg cccaaacaaa ggccgagaga aaaaacgctc ccggctttgg gaaaaaagac
     4501 ttaaacatat gcactcctgc taacttctta tcgttttaac aactgctgtg tattaaccgc
     4561 atgaccacta caaagcgtca gccaaatgaa accgcccatt gtgacccata gcaaggcgct
     4621 tagttcgcca gcctgaccat aagaattgtt tcgacggatt tcacaaacca aagattgttt
     4681 acataagcgg attaacacgc gcaggatcac aaatgcaggc attggtgagc aggaaaaaga
     4741 tcgtgtttac gcgccgttac gtttttaaca ctattattgc actaattcgc cctttgcaat
     4801 ccatcaatga gtagtataaa tacgctcagt taccttcatt caatctatgg acaccaccgt
     4861 tgaaacgtag tctgcttttt tctgccgtgc tgtgtgcggc gtcattgacc tccgtccatg
     4921 cggcgcagcc gatcactgaa ccggagtttg cctctgatat tgtcgatcgt tatgccgatc
     4981 atatttttta cggcagcggt gcaacgggga tggcgctggt tgttatcgac ggtaaccagc
     5041 gcgtctttcg tagttatggc gaaacgcgac ctggtaataa cgttcgcccg cagctggatt
     5101 cggtcgtgcg tattgcttcc ctcaccaaac taatgaccag tgaaatgctg gtgaaattgc
     5161 tcgaccaggg gaccgtaaag ttaaacgatc cgctaagtaa atatgctccg ccaggcgcac
     5221 gcgtgccgac ctacaatggg acgcccatta ccctagtgaa tctggcaacc cataccagcg
     5281 ccctgccccg tgaacagccc ggtggcgcgg cacatcgtcc ggtgtttgtc tggccaacgc
     5341 gcgagcaacg ctggaaatac ctttctacgg cgaagctgaa agccgcgcca ggcagtcagg
     5401 cggcgtactc taaccttgcg ttcgacctgc tggcagatgc actggcgaat gcgtccggca
     5461 agccttatac ccagttgttt gaagagcaga ttacccgtcc attggggatg aaagacacca
     5521 cctacacccc ctcaccggat cagtgccgcc gtttaatggt tgctgagcgc ggtgccagtc
     5581 cgtgcaataa cacgctggcg gcaattggca gcggcggcgt ctattccacg cctggcgata
     5641 tgatgcgctg gatgcagcag tatttgtcat ctgattttta tcagcgcagt aaccaggcag
     5701 atcgcatgca gacgttgatt tatcagcgcg cgcagtttac gaaagtgatt ggcatggatg
     5761 tccccggcaa agcggatgcg cttggtttag gctgggtata tatggccccg aaagagggtc
     5821 gtccggggat tattcagaag acaggcggtg gtggcggttt cattacctat atggcgatga
     5881 tcccgcagaa gaatattggc gcgtttgtgg tggtcactcg ttcgccgttg acgcgcttta
     5941 aaaatatgag cgatggcatc aatgatctgg tgaccgagct aagcgggaat aaaccgttgg
     6001 ttatccccgc atcctgatta atactctgaa ggcaaacggt tgatagcgac cagtttgcct
     6061 ttattcattt cgatatagcc gcctttacgc aaagcagcga gaacttctgc gacgacagaa
     6121 cgcgatatgc gtgtacgttg atggatataa ttcatcacac caatacgtga tcgcaattct
     6181 tcattccagt caatcattga taataatgtt gcgcggattt gttcgtagga attatgccca
     6241 atgagctgca cgtcgcgtaa ttccagaatt ctattttgcc aggctaacca gtaaaaagcg
     6301 tctcgccaga gttgattttg ttcaataagc gtaatggttt gtttggctgg tagatgatat
     6361 cccgtacaat ttccttctga tattaatttg tatggtatat cgtttttcat taaaccatca
     6421 gccagcccca taatataagg ggcctgggta ataccgataa gtacgttttc ttctctacgc
     6481 agagagataa ctccctctaa aataacgaaa gtatcttcgt tattgacatc actggaaaat
     6541 ataacttgct tttcattatt aaactcgaag cgcgtaccgt atctggacaa acatttatcg
     6601 agcttaccaa attcctgaag aggtttaact acagataaca tttgcgcgtc ctttgcagta
     6661 atgcccgtca aatccttgac gggcattatt tagattaaat taccagtatt tcttcggagt
     6721 gaagaatatt accaggtata tttaacaccc acgttcgcgg accagtcttg atctacgtca
     6781 ccaccaccga ggtagttagc atcggtatag gcgctgaagt tcttggtgaa gctgaactga
     6841 gtacccagac caacacgtac cgcagaccct tcagtaccgt tatcgatgga atcaccgttc
     6901 acatcgttat cgttgttaga gtcgtcgtag acgtaagcca gtttgaagta cggagtcaga
     6961 gcctgatctt cgctgtaggt gaaggtataa cctgcatcta cacccagttc ataacgcatg
     7021 ctgtcgtaag actgaccgtc aactttcatg tcgttgctca gctggtagtc atcaccagac
     7081 tggaacagac cagaaacgct gccgtaagga gtcacgtaac cagcatcacc cagtttgaag
     7141 tcgtaaccgg ctttcaagcc gaagccccaa gcgtcggagt tggtgctacc gtcaacgtaa
     7201 gtaccgttgc tcatggttgc agacaggtcg ttgttgaagt gagagtagct caagctacca
     7261 tcaacaaaga cgttgttcgc gaagtgagca gaagagtaga tgtaggcagt ctggctgtct
     7321 tgatctacct gaccagaacg gtcattcatg tcacctttag cgaagcctgc agccgcaccg
     7381 acgatccact tagcgttgtt accgtcaact ttggtatcaa caccgaccat gatgccgtta
     7441 acatcctgat catagttgat ggtgccgttg tcgccgttga agttaccacc gaagtagctt
     7501 acccatgcgc cgccgttatc agccaggcca tgacgagagt tggtcagacg agtaccaacg
     7561 gtgtcttgtt ccaggttcca gatattggtg ttcgcagacg ggatgctcag cgccatgtta
     7621 gcgtagtcgg tcagctccat ctgttgcaga acaacggtgt taccgcgctg ttcagcctga
     7681 taggtgtatg cacccaggtc agctttgtta gcagcagaga aggtcgcgtt ggttttggta
     7741 tcatcgatat agatgagctg cttacctgcg taatcagcaa tcgcaccagc gccagtggag
     7801 ttgtcgatac gaactttgtt gttaccggtt gcagctacaa cgttgtctgc agagctgttg
     7861 tcaacttcgt cgccattgtt aatgtcgttg ttaccattaa cggtcaagtg accatcagag
     7921 ttcatcgcaa taacaccata accgtagtta gctttgctgg tgtcgttggt acgatcgttg
     7981 acgaggtctg cattcaggat gtaatcatta ctatgaatgt caaacacacc tgcgtaaaca
     8041 ttaccgttgt tatctttaac gttgccactg gtcagttcca gagtatcagt tgcaaatacg
     8101 ctggagatgt cattcatgtt ggtgtttaca tccagcacac caccgttagt aacggtaatg
     8161 gtattggcat acagcggtgc atatgcccaa gctgaagcag tatcttcacc gaagttaacg
     8221 gttgagaagg tatcaatggt caggtgatcg gtgttaactt caccgttaac ttccattgaa
     8281 gaaccaccgt tcagagtgat ggtgtcagaa gtcagagaag aatctgcact cacgttaacc
     8341 tgagaaccgc tgttgatcgc cagggtatcg atcagggaag ttttcgtggt ttcccaatca
     8401 gaaccgttgt tcagaacaac atcgaatacg ccgctctgga atgcgttagc gttatcaaca
     8461 cgattccagc catcttcaga gtaagaagcg tacgggttca ggctgtttgg tgcaacgtcg
     8521 tagaaggctt cgtcagcaac aacaacgtca taatcaatat tcgctgaacc aacccatttg
     8581 gtgttgttca gagtcaggtt caggtgatcg acgttctgat cgtcagttgc ccaaccgcca
     8641 tgagtgtaag cagtggtaga gttgtcaatc aggtgacccg tcgcgtcaaa gttagcgttc
     8701 caggtactgt caaatacaac gtcaccaagc aactgagagt tgttgaacgt aacgttgttg
     8761 accatacgat aatcagaagt gtcatcatcg atgaaggcta acgcaacatc atcagcagga
     8821 gatgcgccat tgccgtagtc acttggttca acagaattgc cgaaatgacc acgatctgat
     8881 tgttcgtctt cagtccatga accagaagtt acagtagagt cattaacaac gatgttgttg
     8941 tcccactgat gaccttcggt attgtaggtc gcattaccga ggtcgtgttg aaccaaagta
     9001 atacctgcaa catgggagtt gttgctgatg ttgatgttac tttcaacatc cagagtcaca
     9061 gcggtaccaa gaccagcggt taccaaacga tcataatctg tcttagatgt tttaccgtct
     9121 gcatccagat aagtatcggt gaagtagaag gcgtcaaaat catcgtcaat agtagaatta
     9181 gcgatgttca gagtgaagat gtcgccatca acccagtcgt ggccagtata atcgctaccg
     9241 ttggtccaga cgtagtcgcc ataaccaacc tggtaagatg taaccatacc atgaacaatt
     9301 gagttggaga tgttcagtgt attaccggta tctgtgccat aatcgtggtt cagataataa
     9361 gtagaaatta caccatttac agtgtcgccg ctgaaagtgg tatcaaagta accatcagct
     9421 gcgtggtcat atacataacc accgtaagtc agataatcgt gatcaatttt agtgctgtca
     9481 ccccaattgt agatggtggc tgcgtcatcg tgatacccga aggtgttata agtagctgca
     9541 ttagcctgag aggtgatagc cagagtgcaa gccaatgcta attgtgatac tacaagtttc
     9601 tttttccagg agtgcatttg tcatccctcc tcagggacgt aataagttga tccatcaatt
     9661 atcaatgcat agaaaacata ttatgtttcg cgctaacgat tatgctg
//
LOCUS       DAZPDU010000159         9674 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZPDU010000159 DAZPDU010000000
VERSION     DAZPDU010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9674
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.161"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..1710
                     /locus_tag="ACHURS_003531"
     CDS             <1..1710
                     /locus_tag="ACHURS_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HFR1239170.1"
                     /translation="EMRQTLTLHKGANYISLAQNIQQSGLLSAELQQNGQVQDSISTK
                     LSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLDALVDGPW
                     GGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGARFTWWGED
                     GNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRALVLAWAQ
                     EMNLPCKTLLKGLDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPLADAVANVLTM
                     TLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAILSGLTAEQ
                     STIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVPDILSFGD
                     ELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSNEIDSDAL
                     YLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQLLEKARNE
                     MGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAAGSEWTGM
                     QVK"
     gene            1711..3360
                     /locus_tag="ACHURS_003532"
     CDS             1711..3360
                     /locus_tag="ACHURS_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFR1239171.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPMQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            3365..4141
                     /locus_tag="ACHURS_003533"
     CDS             3365..4141
                     /locus_tag="ACHURS_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFR1239172.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTSGIGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(4215..5399)
                     /gene="atoB"
                     /locus_tag="ACHURS_003534"
     CDS             complement(4215..5399)
                     /gene="atoB"
                     /locus_tag="ACHURS_003534"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HFR1239173.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHIDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAKSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKANSTAEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     SEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(5430..6752)
                     /locus_tag="ACHURS_003535"
     CDS             complement(5430..6752)
                     /locus_tag="ACHURS_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HFR1239174.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVTLIVVMPFITRMMMPKPSDVVSIDPE
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVIPAAQALGADLGKSVMAIAYG
                     EQWMNMEQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            complement(6749..7399)
                     /gene="atoA"
                     /locus_tag="ACHURS_003536"
     CDS             complement(6749..7399)
                     /gene="atoA"
                     /locus_tag="ACHURS_003536"
                     /EC_number="2.8.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HFR1239175.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGVLPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRSCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            complement(7399..8061)
                     /gene="atoD"
                     /locus_tag="ACHURS_003537"
     CDS             complement(7399..8061)
                     /gene="atoD"
                     /locus_tag="ACHURS_003537"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HFR1239176.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            complement(8257..9642)
                     /gene="atoC"
                     /locus_tag="ACHURS_003538"
     CDS             complement(8257..9642)
                     /gene="atoC"
                     /locus_tag="ACHURS_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HFR1239177.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSASWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEVKTASVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
BASE COUNT         2354 a         2484 c         2605 g         2231 t
ORIGIN      
        1 gagatgcgcc agacgctgac gctgcacaaa ggggcgaatt atatttcgct ggcgcagaac
       61 attcagcaat ctggcttgtt aagcgcagaa ctgcaacaaa atgggcaagt gcaggacagc
      121 attagcacaa aactgtcttt tgtggataac agctggcccg ttgaacagca gaaaaatgtc
      181 atgctcggcg gtggcgagaa cgcgctgatg ttgcccgagc aggctagcaa tatccggcta
      241 caaagtagcg aaacgccgca ggagattttc cgcaacaatc ttgatgcgtt agtcgatgga
      301 ccatggggtg gcgtgatcaa caccggtagc cgtctgatcc cgctcagtct cgcctggcgt
      361 tcgcttgccg atcatcaaag tgccgccgct aacgacattc gtcagatgat tcaggataac
      421 cgtctgcggc tgatgcaact ggcggggccc ggagcgcgct ttacctggtg gggtgaagat
      481 ggcaatggtg acgccttcct tacggcatgg gcatggtacg ccgactggca ggccagccag
      541 gcgctcggcg taacgcaaca accggaatac tggcagcata tgctcgacag ttatgccgag
      601 caggcggata acatgccgtt attgcatcgg gcgctggtgc tggcgtgggc acaggagatg
      661 aatctgccgt gcaaaacgtt gttgaaaggg ttggatgaag ctatcgcccg gcgcggaact
      721 aaagatgaag atttctctga ggaagacatc cgcgatatca atgacagcct gatcctcgat
      781 acaccggaat ctccactggc agatgcggtg gcaaacgtct taaccatgac gttgctgaaa
      841 aaagcgcagt tgaagtccac ggtgatgcca caggttcagc aatatgcgtg ggataaagcg
      901 gcaaacagca atcagccgct ggcgcacacg gttgtgctgc tcaatagcgg tggtgacgct
      961 acccagacgg ccgctatttt aagtggtttg accgctgagc aatccactat tgagcgcgcg
     1021 ctggccatga actggctggc gaaatatatg gcgacaatgc ctccagttgt tttgcctgcg
     1081 cctgcgggcg catgggctaa acataagtta actggagggg gcgaagactg gcgttgggtt
     1141 ggtcagggtg tgccggacat tctctctttt ggtgacgaat tatcgccgca aaatgtgcag
     1201 gtccgctggc gtgaaccggc aaaaacggct caacaaagta acattccggt gaccgttgaa
     1261 cgccagttgt atcggcttat ccccggtgaa gaagagatga gctttactct gcagccggtg
     1321 accagcaatg agattgacag cgatgcgctg tatctcgatg aaattacgct taccagcgag
     1381 caggatgcag ttctgcgcta tggtcaggtg gaagtaccgc tcccgccggg agccgacgtt
     1441 gagcgcacaa catggggcat ttcagtcaat aaacccaacg ccgcgaaaca gcaggggcaa
     1501 ttgctggaaa aagcgcgtaa tgaaatgggc gaactggcct atatggtgcc ggtgaaagaa
     1561 ctgacgggaa cggtcacttt ccgccatttg ctgcgcttct cgcaaaaagg gcaattcgtt
     1621 ctgcctcctg ctcgttatgt gcgttcctat gcacctgcgc agcaaagtgt tgcggcaggg
     1681 agtgaatgga ccgggatgca ggtgaaataa gtgaactggc gcagaatcgt ctggctattg
     1741 gcgttggtta ctctgccaac gcttgcggag gagacgccgt tacaactggt gctgcgcggt
     1801 gcgcagcacg atcaactgta tcagctgtcg tcatcaggcg tgaccaaagt ctcagcatta
     1861 cctgactcgc tcaccacgcc gttagggagt ttgtggaaac tgtatgttta cgcctggctg
     1921 gaggataccc atcaaccgga gcagccctac cagtgtcgcg gaaactcccc ggaagaggtt
     1981 tattgttgtc aggctgggga gagcataacg cgcgataccg cgctggtacg ttcctgtggg
     2041 ctctattttg cgccgcagcg gttgcatatt ggcgcagatg tgtgggggca atactggcaa
     2101 cagcgtcagg cacctgcgtg gttagcgtct cttacaacgc tgaagccaga aacctcggta
     2161 acggttaaat cgctgctcga ttcattagcg acgttacctg cgcaaaacaa agctcaggaa
     2221 gtcttactgg atgtggtgct ggacgaggcg aaaatcggtg tcgcatccat gctcggtagt
     2281 cgggtacggg tgaaaacctg gagctggttt gccgatgata aacaggaaat ccgccagggt
     2341 ggttttgctg gctggttaac cgatggtaca ccgctgtggg ttactggcag tggcaccagc
     2401 aaaacggtac taacccgtta tgcgacggtg cttaatcggg ttttgccagt gccgatgcag
     2461 gttgccagtg ggcaatgtgt agaggtggag ctgtttgccc gctatccgct gaagaaaatc
     2521 acagcggaaa agagtacgac ggcagtaaaa cccggcgtac ttaatgggcg ctatcgtgtg
     2581 acgtttacta atggtaacca cattacgttt gtcagccacg gtgagacgac cttactgagc
     2641 gaaaagggca aactgaaatt gcagtcgcat ctcgatcgtg aagagtacgt tgccagagtt
     2701 ctggatcgcg aggcgaaaag tacgccacct gaggcagcga aagccatgac cgtggcgata
     2761 cgtacttttt tacagcaaaa cgccaatcgg gaaggtgatt gcctgaccat ccccgacagc
     2821 agcgccacgc agcgtgtttc tgcttcgcca gcgaccacgg gggcgcgaac gatgaccgct
     2881 tggacgcagg atctcatcta tgcaggcgat ccggttcatt atcatggcag tcgcgctacc
     2941 gaagggacac tttcctggcg gcaagccacg gcccaggcgg ggcaggggga gcgttacgac
     3001 cagatactcg cgtttgctta tcccgacaac agccttagcc gctggggagc tccacgctca
     3061 acctgccagt tattacccaa agcgaaagcc tggctggcga aaaaaatgcc gcagtggcgg
     3121 cgtatattac aaggcgagac ggggtacaac gaaccagacg tgtttgcggt ctgtcgttta
     3181 gtctcaggtt tcccctatac cgatcggcag cagaaacggc tgtttattcg taacttcttt
     3241 acgcttcagg atcggctgga tttaacccat gagtatctgc acctggcctt tgatggttat
     3301 cccaccgggc ttgatgagaa ctatatcgaa acgctgaccc gacaattatt aatggactaa
     3361 tgctatgcga aagatttttc ttccgttgtt actggtggca ctttcgcctg tcgctcacag
     3421 tgaaggtgtg caggaagtcg agattgacgc accgctctct ggctggcatc cggcagaggg
     3481 cgaagatgcc agtttttcac aatcgatcaa ttacccggcg tcgtccgtca acatggcaga
     3541 tgatcaaaat atctcagcgc agatccgcgg caaaataaag aattatgctg cggcgggtaa
     3601 agttcagcag ggccggctgg tggtcaacgg tgccagtatg ccgcagagaa ttgaatccga
     3661 tggttcattt gcacgccctt atattttcac tgaaggcagc aacagcgtgc aggtcatcag
     3721 cccggatggg caaagccggc aaaaaatgca gttttactca acgtcgggta taggaacgat
     3781 tcgtgcacgt ttacggctgg ttctctcgtg ggatacggac aataccgatc tcgatctcca
     3841 tgttgtcacg cccgatggcg aacacgcctg gtacggtaac accgtgctga aaaacagtgg
     3901 tgcactggat atggatgtca cgacggggta cggacccgag attttcgcca tgccagcacc
     3961 agttcacggg cgctatcagg tgtatatcaa ctactatggt gggcgcagcg aaacggaatt
     4021 gacgaccgcc caactgacgc tgatcaccga tgaaggatcg gtcaatgaga aacaggaaac
     4081 atttattgta ccgatgcgta atgctggcga gctgacgctg gtgaaaagtt ttgactggtg
     4141 attaatgggg ttaccttcgc cgtcgatgtt caagaaaacg cccgataact ttcgctatcg
     4201 ggtgttttta ttgattaatt caaccgttca atcaccatcg caattccctg gccgccacca
     4261 atgcacagtg ttgccagccc cagcgtttta tcgcgtgcct gcatggcatg taatagtgtg
     4321 accagaatac gagcaccact ggcaccgata ggatgcccga gcgcgatggc cccgccgttg
     4381 acattcactt tctcagaatc aaagcccagg gttttcccaa cggcgaggaa ctgtgcagca
     4441 aatgcttcat tagcttcgat gagatcaatg tctgccagtt gcagccccgc cagttgtaac
     4501 gctttttgcg tggcaggtac tggccccata cccatcaatg cgggaggcac gccaccgctg
     4561 gcataacttt taatgcgagc caggggggta aggcctgctg ccagcgccgc agattcttcc
     4621 ataatcacca gagcggcagc accgtcgtta ataccagacg cgttcccagc ggtgactgtt
     4681 cctgctttat cgaaggccgg gcgcaatgca cctaacgctt cagccgttga attcgctttc
     4741 gggaattcgt cttgactgaa gacgaaggtt ttcttccgag tgacaacatt taccgggacg
     4801 atttcggctg taaaagcacc ggactcaatt gcggctgccg ctttacgctg tgaatgtagc
     4861 gccagttcat cctgcatttc acgggtaatt ccgtactctt tagccacgtt ttcggcggta
     4921 atccccatat gataaccatg ggtggcgcac atcaggccat cgcgcaggat tacgtcataa
     4981 acctgtccgt ctccaagacg ataaccagag cgtgcttttg catcgagtaa gtagggcgct
     5041 aaactcatat tttccatacc ccccgccaca atgctcttcg cctgacctgc ctgaattgcc
     5101 tgggcggcaa gcgccacact tttaagaccc gaaccacaca ctttattgac cgtgaatccg
     5161 cacaccgttt ctgccagccc gctttttaac agtgcctgac gtgccggatt ttgccccaac
     5221 ccggcttgca acacgttacc cataatcact tcatcaatgt gtagtgaatc gatttttgca
     5281 cgttcaatgg cggctttaat tactgtcgcc cccaggtcga tggcgctggt ggaagcgagt
     5341 gaaccgttaa aactaccgat agcagtacgt accgcactga cgatgacaca atttttcatt
     5401 ttatattcct tctgtctgta gggttgccgt cagaacagcg ttaaaccaat gacgaaaatg
     5461 acgccggaga agagcagggc agtgatgcaa tagcccatga tgtcgcggac accgagtccg
     5521 gcgattgcca gtgctggcag cgcccagaat ggttgttcca tgttcatcca ttgctcgccg
     5581 taggcgatcg ccattaccga tttaccgaga tcagcgccca gcgcctgggc tgcgggtatc
     5641 acgaaaggtc cctgaataac ccagtgaccg ccgccagacg gaacggcgaa gttaatcagt
     5701 gcagaactaa aaaaggtcat taccgggaag gtgtctttgt tcgcaacatt gatgaagaat
     5761 tcggtaatga gtccacccag accagaatgc tccatcatca gttggatccc agcgtagaag
     5821 gggaattgca ccagaatacc ggcagtactg cgtgctgccg cgctgatagc acgcatataa
     5881 gccattggcg ttttatgtag caacagaccc gcaatcataa acatcaggtt gacggtattg
     5941 atggtgatgt tgaagccatg ttcgctgaag tacatcgcca ggtaagcgat accgagtgcg
     6001 ccgatgatca acgtcagaat gcggctttct tccagtcgct cggatggtgg ggcatctttc
     6061 ggtagctgct tttgaaaatc agcctcttcc atgagtagtt ccggatcgat actcaccacg
     6121 tcagacggtt ttggcatcat catgcgggtg ataaatggca tcaccacaat caacgtcaca
     6181 gtgatgaaaa tgttaaaacc actgaacaga gtatcgccca ccgggatcag cccggcgata
     6241 tgctcaaccg ggttgcccgg tgttgcagcc aacagaggca ttgatccaga gaagccgcca
     6301 ccccaggtga gaaaaccaat gtaggcgcag gcaatgagca acggataatc agaaccgggg
     6361 actcgccggg cgacttcacg ggcaaacatt gcgccgacaa ccaaaccaaa tccccagttg
     6421 atgacacaag cgactgaacc gaagaaagtg accagcatga cgccctgtac gggcgtcttt
     6481 gcggcggagg cggcagtacg cagcaaactt ttcaccggag cagagctggc aagggcatga
     6541 ccggtaacga tgataagcgc catctgcata ccaaacgcca gcaagttcca gaaaccgtca
     6601 ccccacattt tcaccatgct gatcggcgtt tgtggtgtta accaaagcgc gatcacgaat
     6661 gttagcaatg tcagcaacat ggcaaagatc agtggatcgg gaagccatcg gctgacaaaa
     6721 cgcgtcataa aacgcgatat gcgaccaatc ataaatcacc ccgttgcgta ttcagatcgg
     6781 cggcgacttc aaaccgagct tctgttttgg cacgcacggt ggctaaatca cacccgtcgg
     6841 caatttcggt gagccacatt ttgccgtcaa taaaacgaaa gacagccagt tcagtaacca
     6901 gcatatgcac cgcatgttgc gcagtgagtg gcatggtgca gctgcgcaaa atttttgctg
     6961 aaccatcttt ggcgcaatgt tccatggcga tgatcacttt gcgcgacccg gtcaccagat
     7021 ccatcgcgcc acccataccg ggcaccattt tcccaggcac tacccagttc gcgaggtttg
     7081 cttcttcgtc tacttgcaaa ccgccgagca cgcaggcatc aatatgaccg ccacggatta
     7141 gcgcaaatga catggcgcta tcaaacatgg ctgcaccggg taaaacaccg cacggttgcc
     7201 cgccagcgtt caccagatct ggatgcgctg tcgtgaccgg gcctaaaccg aggaagccgt
     7261 tttccgattg cagagtgata tgaataccct ccggtaaata attggcgacc attgtgggta
     7321 aaccgatccc taagttaacg atgtcaccat cacgaagctc ttgcgccaca cggcgcgcaa
     7381 tacgttgttt cgcatccatt atttgctctc ctgtgaaacg atgatgtggt cgataacggc
     7441 accaggggtg acaatatggt caggttgcag ctcgccggtt tcgaccagtt catctggctc
     7501 taccagcgtg atatcagccg caagggctat cagggggtta aagttgcggg cgctaagttg
     7561 ataggtcagg ttgccaagtg tgtcgcaacg atgagcgcga attagcgcca ggtcggcgcg
     7621 cagtgggcgt tcgagcagcc aggttttacc gtcgagtgtc agtgtctgtt tgccttcctc
     7681 tacgacggtg ccgacacccg ttggggtgag aaaaccacca agtccagctc caccacagcg
     7741 aatttgctcg attagcgtac cttgcggcac cagaacgacg tccatctcac cagatatcat
     7801 gcgccgacct gtttccgggt tggtgccgat atgtgaagca atcactttgc ggactcgacc
     7861 attgacgatg agcggaccga tgccggtatc aacaaacgcg gtatcattgg ctatcaatgt
     7921 caggtcgcga acaccagatt ccagtaatgc ttcaaccagg cgggatggag tgccaatccc
     7981 cataaatccg cccaccatga tggtcatgcc gtcacgaaag aagccggtgg cgtcttgtaa
     8041 tgtcatcaat tttgttttca ttttttatcc ctcttgcata caccgttgtg ggtacagtca
     8101 ggcaatagca aggggagtgc cagaatgaga ttattggatt tagttattta actaattgaa
     8161 tatgaaggga taaattggat ggggatttat aaggagaatg agcggctatg cagaaaattg
     8221 cgcactgtgc aaatttctgc atagcaagtt ttggtgttat acatccgccg gatcgatacc
     8281 gtattcctgg agtttataca tcaatgcacg gcgactgatg cccagcatta aagcagtgcg
     8341 ggttcggttt ccttcttgtt gttccagcac ttccataatg atgcgttttt cgacgcgttt
     8401 aatttcctct tttaaattac gctcaccgac agaggctgtt tttacctcgc cagcattaca
     8461 gactggctga cgaatctgtg gcggaagatc ctcagaaaaa atgatcgggc ctgaattcat
     8521 cacgacggcg cgttcaataa cgttagaaag ctctcgaata tttcccggcc atgaccaggc
     8581 ggtaagcagt gacattgcca tcggatcgat gtcgataata tcgcgctgat tctcactact
     8641 gaatttttgc aaaaagtgat tagctaacag ggaaatatct tcccggcgat cgcgcagagg
     8701 cggcagtatt aaatgaataa cgttaaggcg ataaaagaga tcttcacgga aggtgccttc
     8761 ttttaccatt gcctgcaagt cacggttggt ggcagcaatg atgcggatat caacttttat
     8821 ggtctgatgg ccgccaatcc gttcaaattc ccgttcctgt agaatgcgta gtaatttggc
     8881 ttgtagtacc agcggcattt cgccaatttc gtcgaggagc agagtacctt cgttggctcg
     8941 ttcaaataat ccctgacgca aggtttgtgc accagtaaat gcaccttttt catgaccaaa
     9001 cagttcactt tcgagcaacg attccggcag cgccgcgcag ttgactttaa tgaacggccc
     9061 ctttgcccgc cgcgaattgt agtgaatcgc tctggcaatc aactctttcc cggtgccgct
     9121 ttcaccgcta atcaagacgc tggcctgaga aagggcaatt ttggcggtgt ctttgcagat
     9181 gtccatcatc gccgggctgt tggtgagaat gtgcccccat tgccagctgg cgctcagtgc
     9241 ctggtgcaga tgacggatct cttttttcat tgactggagt tgtaaagcgc gctgaacgat
     9301 taaattcaac tcatcgagat caaacggttt aataacatag tcgaaggctc cgcagcgtag
     9361 cgcttcgacg gcggtttcca cttccgcata ggccgtcatc agaataacgg gtgtccgggt
     9421 ctcatggctg cgcatctcct ttagtgcctt gatgccgtcc atctctggca tgcggatatc
     9481 catcaacacc acatcagggt gaatatcggc aaacaggtgt aatgctgtgc gtccgttgtt
     9541 cgcacaatgt gtttcgaatc cttgtagtgc aaaagcggtg ctcagcatac ggcgaacatt
     9601 atcttcatca tccacaataa ggatgcgatt aatagcagtc atacagtctg atttccctgc
     9661 gggttgatcg gtaa
//
LOCUS       DAZPDU010000160         9645 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZPDU010000160 DAZPDU010000000
VERSION     DAZPDU010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9645
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.162"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..667)
                     /gene="ydcF"
                     /locus_tag="ACHURS_003539"
     CDS             complement(<1..667)
                     /gene="ydcF"
                     /locus_tag="ACHURS_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFR1239178.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIQ
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDD"
     gene            complement(939..4841)
                     /gene="hrpA"
                     /locus_tag="ACHURS_003540"
     CDS             complement(939..4841)
                     /gene="hrpA"
                     /locus_tag="ACHURS_003540"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFR1239179.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKIRKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQVMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDKLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            5042..5647
                     /gene="azoR"
                     /locus_tag="ACHURS_003541"
     CDS             5042..5647
                     /gene="azoR"
                     /locus_tag="ACHURS_003541"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFR1239180.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(5698..7014)
                     /locus_tag="ACHURS_003542"
     CDS             complement(5698..7014)
                     /locus_tag="ACHURS_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HFR1239181.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDSNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGACACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGYCKTVDEAISFIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(7004..8761)
                     /locus_tag="ACHURS_003543"
     CDS             complement(7004..8761)
                     /locus_tag="ACHURS_003543"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFR1239182.1"
                     /translation="MENSRISGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHIVDELAMPDTAFYAWDARGHGKTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALTLWHR
                     LRGLFFINSYVKRRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFIRRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGT
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHVKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKLWVMRVRTQGEMDLLVHDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(8777..>9645)
                     /locus_tag="ACHURS_003544"
     CDS             complement(8777..>9645)
                     /locus_tag="ACHURS_003544"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFR1239183.1"
                     /translation="ALLILATLINRFLLWRLPERKGGEVTLRIRTWWGIVICFSLVIS
                     GPRWMTLAFFALISFLALKEYCMLISVHFPRWLYWVIPLNYLLIGFNCFELFLLFIPL
                     AGFLILATWRVFVGDPSGFLHTVSAIFWGWIMTVFALSHAAWLLMLPSMNIQGGALLV
                     LFLLALTASNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMIASMIIGPLLT
                     PLNTLQAILAGLLIGISGFCGDVVMSAIKRDVGVKDSGKLLPGHGGILDRIDSLIFTA
                     PVFFYFIRYCCY"
BASE COUNT         2448 a         2628 c         2273 g         2296 t
ORIGIN      
        1 tatcatcgcg taaacgcggc agctcgccag tgagtagtga gagataacgc tcaaccggcc
       61 ataatccttg taactgatta ataaagatta cactgtctgc gttatttcct aactgaggaa
      121 cgaatccggg ataacttaac cagcgtggtg catcgggatt gtccccagtc atacggcgga
      181 acgtcgccat cgtgcgccgc tgcatggtgg ggtcctgaac aacgatagcc gtatgaactc
      241 gttctacggc ctgattcaat agcgcgatgc taaagcgtgc gttttcaccg cagtttgttg
      301 actggtcttc aatccagatt ttttcatgcg gaatgtgcca gaactgatga gcgatatccg
      361 cctggatggt cgcttctgct ctgccagtgg tgcggatagt gttgtagtgc ggatgctgtg
      421 cgatggcgct atacaaaaaa gttgtcgagt gaccgatacc accactaatc agtaaaggaa
      481 tttgctgatc gcgagcaatc ttacatgccg cgtcgatagt cggcataacc gcattgcctg
      541 caaggatcac gcaatcggcc tgatacggca cctcaccgga gaaatcatcc tgcgccagcc
      601 actgtccgat aacatttatg gcatctatag ttgccggcga aagcgtcgga aacggggtaa
      661 tgttcatggt tcctccttat cgttccctta agaatactgc gtgagataat agtgagaagc
      721 ggagcactct gaaaggagga agtcgtattt ccttaaccgc cactccgcaa gcctgacata
      781 tcttttaagg aaatgctgta gataaacatt attcatcttg cagcagagaa ttgtattttt
      841 ctgaattcct gaatttttac ccttaccgtt ttagaattaa taaaaaaccc ggcgctaaat
      901 gcttaccggg ttttttcttt atcaggcaaa tagcagggtt aaccgctaat ctgctccatc
      961 gcctgcaaaa tacgcttatc tgaaatcgga taaggcgtac caagttgttg agcgaagtaa
     1021 ctaacacgca actcttctat catccaacgg atctctttca cgtcttcatc ctcacgacgt
     1081 gcaggcggca gtttgttgat ccattgctgc cacgcctgct ggacgttttc gactttcagc
     1141 atctgagcac ggtcgcgatg tggatcaacc gccagttttt ccagccgttt ttcaatcgcc
     1201 tgcaaatatc gcagcgtgtc gcccagccgt ttgaagccgt taccagtgac aaaaccgcga
     1261 tataccagcc cgcccatctg cgctttaatg tcagaaagcc ccagcgccat cgtcatatcc
     1321 acccgcccct tcagacgttt gttgatattg aacactgccg taaggatttg ctcgacctgc
     1381 ttcgcaatat ccaccaccgt gtcgttcagt tcggcacgca ctttttcatg cagcgcagca
     1441 aagccttctt ccgtccagac cgggccacca ttggcgtcga tcaatttatc cacaccgcag
     1501 gagatacaat cgtcgatcag ctccagcact ttgccatacg ggttaaagta cagccccagc
     1561 ttggctttgt ttggtaactt ctcatgtaaa tacttgatcg gcgagggaat attcagcagc
     1621 agtagacggc gaagaccgtt ccacattacc tgtttttgct ccagcggatt atcaaacagt
     1681 ttgatcgcca cactgtcgcg ttcatccact agcgctggcc acgccttcac tttgtagttg
     1741 ccacgcttct gttcgtagct ttccggcagc tgaccaaaac tccagatatg cagtccgctc
     1801 tgctcgatac cgtcatccgc caccgcagac agcgtttcct gcactttgcc tttcagcgca
     1861 tctttcagat cttgtagcga gcgcccttct tttagcttct tgtttttgtc atccaccacg
     1921 cggaaggtga ttttcaggtg atcgggcacc tgatcccagt gccagtcttc gcggtcaacg
     1981 gtaacgccgg tcatccgccg taactcgcgc tcaaggctgt cgagcaacgg taactccagc
     2041 ggtgtgacgc ggcctaaaaa cgcttcggcg tagtttggtg caggcacaaa attacggcgt
     2101 accggtttcg gcaacgattt aatcagagca atcaccaatt cgcggcgcag accggggatc
     2161 tgccattcaa acccgctttc ctcaacctgg ttaagtaacg gcagcggaat atgtacggtc
     2221 acaccgtccg cgtccgcgcc tggctcaaac tgatagctca aacgcagctt gagattgccc
     2281 tgatgccaga agttcgggta atccagcttt ctgatttttt ccgccccttc tttgatcaac
     2341 atgctctttt caaagttgag caaatcaggc gtttcgcggc tgactttttt ccaccagcta
     2401 tcgaagtgac gagcggagat tacatcgtgg ctgatgcgct ggtcgtagaa ctcaaacagc
     2461 gtttcgtcat caaccagaat atcgcggcga cgtgatttgt gttccagctc ttccacttcg
     2521 gcccgcagtt tcaggttttc acggaagaat gcgtgacgcg tctgccagtc accttccacc
     2581 agagcgtggc gaataaagag ttcacgacat aacgccgaat cgatctggct gtagttgacc
     2641 ttgcgcgcag caacaatcgg caaaccataa acagtgactt tttccgttgc catcaccgcg
     2701 ccctgcgccc gttcccagtg cggttcgctg taggtgcgtt taatcaaatg ctgagcaacg
     2761 ggttctaccc attccgggtc gatacgcgca gcaatgcgcc cccacaggcg gctggtttct
     2821 accagttccg ccaccattac ccatttcggc ggctttttga ataaaccaga accggggaag
     2881 atggagaaac gcgcgttacg tgcaccggta tattcctgtt tatcggcatc tttcatgccg
     2941 atatgggaaa gcaaaccggt cagtaaggcg atgtgaattt cacgatactc cgccggttcg
     3001 ctgttgaccg gaatgccaag ttctttcacc acctggcgca actgggtgta gatatcctgc
     3061 cattcgcgca cgcgcagata gttgaggtaa tcggtacgac acaggcgacg gaaggcgttg
     3121 gaagaaagcg ccttttgctg ctcgccaaga taattccaca gatttacaaa cgcgaggaag
     3181 tcggattctt tgtcgtggaa gcgacgatgt ttttcgtccg atgcctgctg tttgtccatc
     3241 ggacgttcgc gcggatcctg aatggagagc gcggacgtga taatcatcgc ctcacgcacg
     3301 cagccatgtt tttgcgcttc cagcaccata cgcgccagac gtgggtcgac aggcaactgc
     3361 gagagctggc gaccgagcgg cgtcagttta taggcgctgg cctgttcatc cgtggtgatc
     3421 gcgcccagtt cttcgagcag acgcacgcca tcctggatat tgcgtttatc cggtgcttcg
     3481 acaaacggga acgcagcgat atcgcccagc cccagcgcgg tcatctgcaa aataaccgag
     3541 gccaggttgg tacgcagaat ctccggatcg gtaaactccg ggcgcgagag gaaatcgtct
     3601 tcggaataga gacgaataca gatcccttcg gacacacgac cacagcggcc tttacgctga
     3661 ttggcagacg cctgcgaaat gggttcaatc ggcaaacgct gcactttggt gcgatagctg
     3721 tagcggctga tacgcgctgt accggggtcg atgacgtatt taatccccgg tacggtcagc
     3781 gaggtttccg cgacgttggt cgccagcaca atgcgccgtc cgctgtgcga ctggaacacg
     3841 cggttctgtt cgctgttcga aagccgcgca taaagcggca agatttcggt atggcgtaag
     3901 ttcagcttgt tcagcgcatc ggcggtatcg cgaatttccc gctcaccact cataaagatc
     3961 agaatgtcgc cagggctttc ctgactcagt tcgtctacgg cgtcaaaaat cgcctgcaac
     4021 tgatcgcgct cggtgtcatc ggcttcttca acaatcggtc gatagcgcac ttccaccgga
     4081 taggtccgac cggagacttc gataatcggc gcattattaa agtggcgcga aaagcgttcc
     4141 gggtcgatag tcgcggaagt gataatgatt tttaggtcag gacgccgcgg cagcaactct
     4201 ttcaaatagc cgagcagaaa atcgatattc aggctgcgtt cgtgcgcttc gtcaataatg
     4261 atggtgtcgt actgcatcag caggcggtct tgctggatct ccgccagcag gataccgtcg
     4321 gtcatcagct tgaccatcgt gttatcactt acgtgatcgc tgaaacgcac tttgtaaccg
     4381 atgcaaccgc ccggctccgt tttcagctct tccgcaatac ggttcgccac cgttcttgcc
     4441 gccaggcgac gcggctgggt atggccgatc agccctttaa tcccgcgccc cagctccata
     4501 cagattttcg gtaactgagt cgttttacca gaacccgttt ccccggcgac gatcaccacc
     4561 tggtgatcac gaatcgcttc gagaatgtcc tgttttttct gactaaccgg taaattgtca
     4621 ggataagtaa tttccggtcg tgccgcttca cgcagcagga ctttccctgc cgcctggtca
     4681 atctctttcg ccatctcctg gaaaatggcc tgttgtgcat caggattttt aaccttcttc
     4741 acgccgtgca gacggcgaga aaaacgcagt ctgtcacgca gcatcagcga atccagccgc
     4801 tgctgcaagg ccgtaaaggt caatttttgt tgttctgtca taacgttagt gggcagtagt
     4861 ctgcctgatt aattttcttt gaaatgatag atatagagta ccacatcacg gctttctatg
     4921 cgttgttcaa taaattcgaa cataggcttc gatttattgc gctatctctg tggcaggatt
     4981 gtgaataaag tgtcaacaag caacggggca tcgcccattc aaacatctat aaggaaacac
     5041 catgagcaag gtattagttc ttaaatccag catcctggca gggtactctc agtctaatca
     5101 gttgtccgat tattttgttg aacaatggcg cgaaaagcac tccgctgatg aaatcaccgt
     5161 tcgcgacctg gctgcaaatc cgattccggt actggatggc gaactggttg gtgcactgcg
     5221 tccgagcgat gcgccgctga ctccgcgtca gcaggaagct ctggcgcttt ccgatgagct
     5281 gattgccgag ctgaaagccc acgacgttat cgttattgcg gcaccgatgt ataacttcaa
     5341 catctcaact cagttgaaaa actattttga cctggttgca cgcgcaggcg ttactttccg
     5401 ctataccgag aacggtccgg aaggtctggt aacgggtaaa aaagccatcg ttattaccag
     5461 ccgcggtggg atccacaaag atggaccaac ggacctggtg acgccgtatc tgtccacgtt
     5521 cctcggcttt atcggcatta ctgatgtgaa atttgtcttc gccgaaggga tcgcatacgg
     5581 tccggaaatg gcagcgaaag cgcagtctga cgcgaaagca gccatcgaca gcattgttgc
     5641 tgcataaccg gacacgatct tgatgtggac tgtagtccag tccacatccc aatccactta
     5701 cctgttttcc cataatttca gcatcgcttt gtgatcttcc ttaagcacaa tatgcgagcg
     5761 tctggctcga ataaagctaa tcgcttcatc aacggtttta cagtatccgt aacataacag
     5821 ccatgccgcc actaccagcg cactgcgcga taatcccagt gcgcaatgga ccagaacgct
     5881 gccttgctct tcgcgtaatg tttccagcat cgccacggcc tgtcggagct ccccctcttc
     5941 cggaaccacc agatctagca tcggtacaca gaaatagagt cgatcttttg tcgctcgtcc
     6001 acgagggaat tcaaaggtga cgtccagaac cgcattctgt gccggaatat gtcgtggaaa
     6061 cgcccctaaa taaacaccag cagtcatttt gctcaccggc tccaggcgac gacaaaacca
     6121 acgcatagat agccacatcc cgatgcgcca gggcaatgtc agccagtaaa cggcgggcgg
     6181 gagtttcccc tgactatctt tccctgttgt tatcgcgcca agcccaccgt acccacggcc
     6241 aataatgagt agcgataata ctggccaaca taaccagact gaccaccata actgaaccat
     6301 catcattagc tccatcaaca caatgcacgc gcacgcgcct acgacatatg gcagtgctat
     6361 tttgattcga cgttgatcag gtttctgata attccaccga cggtcgacgg gcaccatcca
     6421 gtcaatcaac atacctaccg ccagccctgt gatgacatca ataaaatgat gctgccatgt
     6481 cgtcagcgtc gaaatggcga tgagtaaaaa ccatccgccg cagactttac gccacctcac
     6541 agccagatgc tgacgaaagt gacgccaaag tagccagcag agaataatat gcagcgaagg
     6601 agactggtta taaggcagat caaacagttc aagttgcgaa aatagccatc ccgtcacccc
     6661 actcacttca ggacggataa aactaaactt cagcggatag agcaaaaaac cgcagcaggc
     6721 cattaccgtt gccagaataa gccggtggac aagtcggcgc tgttcgaatg tcgtgctaca
     6781 aacgaacagc gaaaatccat ataaaagatc cagactccag taaggaacaa tagtccaggg
     6841 aagaaaaggg atcgccgttt cccaaccgaa tacctgactg ggaatatcat ggctattact
     6901 gtcctgaacc gcggtgaact gattaagaga tccataggtg aagaagaaaa acggggccag
     6961 caacaataac cagccagctc cttgtagcaa tacgttccgg cgttcagtta tcacgacgca
     7021 ccgccatcga aaccgtaaaa atgccccact catcaatccg ttgtgtgcat ttatcaaatc
     7081 cagcatcatg cacgagcaaa tccatctccc cttgcgtacg cacgcgcatt acccacagtt
     7141 taccatcttt atgactggtt aacaccccgg caatcagctc cagttgtggg tgccacggct
     7201 gcccggtgta gatgagaatg ccacccggtt cgatggcatt ggcaagacct gcgagtgagt
     7261 tttttacctg ctcattttcg ggaaacagct catacagtcc agagacaatc gccagcgtag
     7321 gccgcggagt taacgcgctg agttcctccg ggttaaacgc atcgccctgt tcaaaacgca
     7381 cccgcccaga cattccccgt tgggcaatca tctcttgccc ctgtgcaaca tttaactcac
     7441 tgtaatcacg taataaaata tcgcttacgg caggctcgtt tgccagtgca tccagtacat
     7501 agcgcccatg ccctgcggca atgtcaacca cgcggacggc taaacctttg acgtggagat
     7561 cggcaacggc ttgtttaatc agtatttgta aatgagtttt gcgctggcga ataccgcgcc
     7621 agccaacact gttcaggtag attttgtcga ttaagcgccc gaatgcgtta ctgccttgcg
     7681 gttgattttg atagacataa tccagcgaac tgcctgaatc aaagccagtg ctcattccga
     7741 gatgcaggcc cgcagagtgc gtcccgaaca atttcatcgc tttacgcata aagcgatacg
     7801 ccaaatcaac cggcgataat ggcacgggtc cacctgaaag gaggcgccag cgatccgctg
     7861 atggtccagt gcggtcttca tgctgataat caaatttttg tgacttgtta gcatataaac
     7921 gacgaataaa gctttgcatt ttttcaaatg cctgcgccct gttctcttca cccaacgtgt
     7981 cgtgataaaa gcctggcagc agatgcagct ctttcagagg gctacgtaat ctctgataaa
     8041 aatcaatctg cggttggcga tgcaccacat agtcatcgcc tgatatcaga agttgcgtgg
     8101 ggagcgtaat cgccgccgca tctctaataa tacgttcaga cgttttatag agatcgagca
     8161 agatattgac ggcaatcgcc cgtgtgatca gcggatcatt attgaaactc gccacccgtt
     8221 gccgatcgtg ggtcaaatag cgtcttttca cataggaatt aataaaaaac agaccacgca
     8281 gacgatgcca tagcgtcagc gcaggacgtg ccagcggcac atacaattta accttaaagg
     8341 ccggagaagc cagcaccagc ccgcgaattg caggcgcata atcatgaacc catgtggcaa
     8401 ccagcactgc gccgacgctt tgcgcgatca caaccacctc ttccagtcca acctggctgt
     8461 cgctggcagc aaaacggaca aattcatcga catcctgcac tgaacgcgca agagatgggc
     8521 tataaccacg cggccccgaa gtttttccat gcccccgggc atcccatgca taaaaagcag
     8581 tatctggcat cgccagttca tcaacgatat gttgtagacg accagaatgt tcatggccac
     8641 gatgaaataa gacgatgacc tttttcgccc ctggctgtaa agcgggccag tgccgataaa
     8701 acaacgctgt attatcactg gtggtaaaaa aatgttcccc agagatgcgt gaattttcca
     8761 ttttctctat tttccttcag taacagcagt aacgaataaa ataaaaaaat accggagcgg
     8821 tgaaaattaa tgagtcaatc ctgtcaagga ttccgccatg tccaggtaat agtttcccgc
     8881 tatctttaac accaacatct cgtttgatgg ctgacatcac tacatcgccg caaaaaccac
     8941 taataccaat taacaaaccc gctaatattg cctgtaatgt atttagcggt gtcagtaacg
     9001 gtccaatgat catcgacgct atcatggtgg tgattacgcc gccgaccaga ccttccagcg
     9061 tttttcccgg gctgactttg gggaccactt ttcttctgcc gcaggatttc ccccataaat
     9121 actgtgcaat atcgtttgac gcggttaatg ccaaaagaaa taacaccagt aaggccccgc
     9181 cctggatatt catggatggc aacattaata accaggcggc atgactcaag gcgaaaacgg
     9241 tcattatcca gccccaaaaa atggcgctca cggtatgcag aaaaccggag gggtcaccca
     9301 caaaaactcg ccaggtggct aatatcagaa acccagccaa aggtataaat aacagaaaca
     9361 gctcaaagca attaaaaccg ataagcaaat agttaagagg aataacccaa tataaccacc
     9421 gcggaaaatg tacggatata agcatacaat attctttcaa tgccagaaaa cttatcagcg
     9481 caaaaaacgc caacgtcatc cagcgtggac ctgaaatcac cagtgaaaaa caaatgacga
     9541 tgccccacca ggtacgaata cgtaatgtaa cctcaccccc ttttctctcc ggtaaccgcc
     9601 atagcagaaa acggtttatc agcgttgcta aaattaacaa cgcga
//
LOCUS       DAZPDU010000161         9622 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZPDU010000161 DAZPDU010000000
VERSION     DAZPDU010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9622
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.163"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            1..2200
                     /locus_tag="ACHURS_003545"
                     /pseudo
     CDS             1..2200
                     /locus_tag="ACHURS_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5682 family protein"
     gene            2193..3329
                     /locus_tag="ACHURS_003546"
     CDS             2193..3329
                     /locus_tag="ACHURS_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFR1239184.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDMLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            3326..5326
                     /locus_tag="ACHURS_003547"
     CDS             3326..5326
                     /locus_tag="ACHURS_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFR1239185.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     DALIATFSDGVRTQLANGQALKEAQCSCGANGMCRHRVMLVLSYQRLCATTQSTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDMRFYSRSS
                     IRFARCDCIESTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSIGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            5451..5912
                     /locus_tag="ACHURS_003548"
     CDS             5451..5912
                     /locus_tag="ACHURS_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFR1239186.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(5953..6423)
                     /locus_tag="ACHURS_003549"
     CDS             complement(5953..6423)
                     /locus_tag="ACHURS_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFR1239187.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(6470..7189)
                     /gene="btsR"
                     /locus_tag="ACHURS_003550"
                     /gene_synonym="yehT"
     CDS             complement(6470..7189)
                     /gene="btsR"
                     /locus_tag="ACHURS_003550"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFR1239188.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(7186..8871)
                     /gene="btsS"
                     /locus_tag="ACHURS_003551"
     CDS             complement(7186..8871)
                     /gene="btsS"
                     /locus_tag="ACHURS_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFR1239189.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISSP
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            9093..9158
                     /locus_tag="ACHURS_003552"
                     /pseudo
     CDS             9093..9158
                     /locus_tag="ACHURS_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9386..>9622
                     /locus_tag="ACHURS_003553"
     CDS             9386..>9622
                     /locus_tag="ACHURS_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR1239190.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEW"
BASE COUNT         2354 a         2532 c         2469 g         2267 t
ORIGIN      
        1 gcggccacga tacgtattga ttgaaggccc ggctgatttt aatgaccggg tagacgaact
       61 gtttttagcc caccagcttc cggtagctat ttacagttat tgccagtatc aggacggtgc
      121 agcccccggg cgtggtgcct ggacgccatt tgctgaattt tcgccggagt ggcaggcgct
      181 acaagccgca cgtcgtattc aggcacaaac ttacttcatc gatttgcctt gctgggcgca
      241 gagtgaagaa gtggacgatt cgcctgacac gcaagaggaa agccaggcct tgctgctgcg
      301 tgccacccgc atggataaca gcgacaccct gtgggatcac ttgttcgaag atgaaagcca
      361 gcaaactgca ttaccctctg cactggcgca ctattttgcc caactgcggg gcgattcccc
      421 cggcgatgca ctcaatcgtc agcgcgaagc ctttatggcc cgctggattg gatgggcgat
      481 gcagcaaaac aatggcgacg tgttagtcgt ctgcggtggc tggcacgctc ctgcactggc
      541 aaaaatgtga cgcgaatgcc cgcaggacat taacacgccg gaattgccct cgctggcaga
      601 tgccgttaca ggttgttatc tcacccccta cagtgaaaag cgccttgatg tgctggcagg
      661 atacctttcc ggtatgcctg ccccggtctg gcaaagctgg tactggcagt ggggcttaca
      721 gcaggcggga gaacaactgc taaaaacggt tcttacccgt ttgcgccagc acaaattgcc
      781 tgcttcgacc gcggatatgg ctgccgctca tctgcatgcg atggcgctgg cacagttgcg
      841 cggacataaa ctaccgttac gcactgactg gctggatgcc atagcaggct cgctgattaa
      901 agaagccctg aacgcgccgt tgccgtggag ctatcgcgga gttattcatc ccgacaccga
      961 tccgattctg ctaacgttga tagacacatt agcgggtgac ggattcggta aacttgcccc
     1021 ttctacgcca caaccgcctc tgccaaaaga tgtcacctgc gaactggaac gtaccgcaat
     1081 ctctcttccg gcggagctta ccttaaatcg ctttaacccc aatgggctag cgcaaagtca
     1141 ggtgttacat cggctggcaa tactggagat cccagggatt gtacgccagc agggaagcac
     1201 actgacactt gcaggcaacg gtgaagaacg ctggaaatta acccgcccgc ttagccagca
     1261 tgcggcattg attgaggccg cctgctttgg tgccacactc caggaagccg cacgccataa
     1321 attagaagcc gatatgctgg acgcgggtgg aatcggcagt atcaccacat gtcttagcca
     1381 ggcggcgtta gcgggtctgg cgtccttcag tcaacaatta ctggagcaac tcacattatt
     1441 aatcgcccag gaaaatcaat ttgccgaaat gggccaggcg ctggaagtgc tatatgcctt
     1501 atggcggctg gatgaaatta gcggtatgca aggcgcgcag atattacaaa cgacgttatg
     1561 cgcggctatc gatcgcacgc tgtggctgtg tgaatctaac ggcagaccag atgaaaagga
     1621 gtttcacgct cacctgcata gctggcaggc gctttgccat atcctgcgcg atctacatag
     1681 cggcgttaat ttacccggcg tttttctttc tgcggcggta gccttactgg agcgccgcag
     1741 tcaggcgatt catgtcccgg cgctggatcg cggcgcggct cttggcgcac tgatgcgtct
     1801 ggaacatccc aacgccagtg ccgaagcggc gctgacgatg ctggcgcagt tatccccggc
     1861 acaatccggc gaggcgctgc acggtttgct ggcgctcgcc cgtcatcaac tggcctgcca
     1921 accagcattt atcgccggtt tcagtagtca tttaaatcaa ctgagtgacg acgattttat
     1981 taacgccctg cccgatttgc gcgcggcgat ggcctggcta ccgccacgag aacgcgggac
     2041 actggcgcat caggtgcttg agcattacca gcttgcgcag cttcctgttt cagcgctgca
     2101 aatgccgttg cattgtcctc cgcaagccat tgcacatcat caacaactcg aacagcaggc
     2161 actggcatcg ctgcaaaact ggggagtttt ccatgtctga actgaacgat cttctgacca
     2221 cccgtgagct acaacgctgg cgattaattc ttggcgaagc ggcagaaacg acgctttgtg
     2281 ggctggatga caacgcccgg cagatagacc acgcgctgga atggctgtat gggcgcgatc
     2341 ctgaacggct ccagcgtggt gaacgctccg gtggattagg tggctcaaat ctcaccactc
     2401 ctgagtggat caacagtatt cacacgctgt ttccgcaaca ggtgattgag cggctggaaa
     2461 gcgatgccgt actgcgctac ggcattgaag atgtggtgac gaatctcgac atgctggaac
     2521 gtatgcagcc ttctgaaagc ctgctacgcg ccgttttgca caccaaacat ctgatgaatc
     2581 ccgaagtact ggctgccgcc cgccggatag tgcgccaggt tgttgaagaa attatggctc
     2641 gactggcaaa ggaagttcgt caggcttttt ctggtgtccg cgatcgccgt cgccgctcat
     2701 ttattccact ggcgcgaaac tttgatttca aaagtactct gcgcgctaac ctgcaacact
     2761 ggcacccgca acacggcaag ttgtatatcg aatccccccg ctttaacagc cgcattaaac
     2821 gccaaagcga acaatggcaa ctggtcttac tggttgatca aagcggatcg atggtggact
     2881 cggtgatcca ctctgcggtg atggcggcct gtttgtggca gttacccggc attcgtaccc
     2941 atctggtggc gtttgacaca agcgtggttg atctcacggc agacgttgcc gatccggtag
     3001 agttattaat gaaagtacag ttgggcggcg ggaccaatat cgccagtgcc gtggagtatg
     3061 gtcggcaact tattgaacaa ccagcaaaaa gcgtcattat cctcgtgagc gatttttacg
     3121 aagggggttc atcatcattg ctgacgcatc aggtgaaaaa gtgtgtccag agcggcatca
     3181 aagtgctggg actggctgcg ctcgatagca ccgcaacacc ttgctatgac cgcgatacgg
     3241 cccaggcgct ggttaatgtc ggcgcacaaa tagccgccat gacgccgggc gagctggcat
     3301 catggcttgc ggagaatctt cagtcatgaa ttcactacgt ccggaattat tagaactgac
     3361 accgcaggcc ctgacggcgt taagcaatgc cggttttgtt aagcgcagtc ttaaggaact
     3421 ggaaaatggc aacgtcccgg agatcagcca tgagaacgac gctttaatcg ccaccttcag
     3481 tgacggtgtc cgtacccagc tggcgaacgg ccaggcactg aaagaggctc agtgcagttg
     3541 cggggccaac ggtatgtgcc gtcatcgcgt gatgctggtg ttaagttatc aacgactttg
     3601 tgccaccact cagtctacgg aaaaagaaga agagtgggat ccggcaatct ggctggaaga
     3661 actggctacc cttcccgatg ctacccgcaa acgcgcacag gcgctggtcg ctaaaggcat
     3721 caccattgag ttgttctgtg cgccgggtga aattccctct gcccgcttac cgatgagcga
     3781 tatgcgtttt tattcccgca gcagtattcg tttcgcccgt tgtgattgta ttgaaagcac
     3841 actttgcgaa catgtcgtac tggcagtaca ggcctttgtc caggcaaaag cgcagcaagc
     3901 ggaattgact catttaatct ggcagatgcg cagcgaacac gtcacatcat ctgacgatcc
     3961 gtttgccagc gaagaaggca aaacgtgtcg tcaatatgtt caacaactaa gccaggcatt
     4021 atggctgagc ggcatcagcc agccgcttat tcactacgag gccgctttca gccgcgcgca
     4081 gcaagcagcg gaacgctgca gctggcgatg ggtgagtgaa tcgctacggc aactgcgcgc
     4141 cagcgttgat gccttccacg cccgcgccag ccactatcat gccggagaat gcttacgtca
     4201 gcttgcggca ttaaacagtc gattaaattg cgcacaagag atggcccggc gcgacagtat
     4261 tggtgaagtt cctcctgtgc cgtggcgcac ggtcgttggc tctggcattg ccggagaagc
     4321 aaagcttgat catctgcggc tggtgtcttt aggtatgcgt tgctggcagg atattgagca
     4381 ttatggttta cgcatctggt ttaccgatcc cgacaccggc agtattttgc acctttcgcg
     4441 cagttggccg cgaagtgaac aggaaaactc accggcagct acgcgtcggc tgtttagttt
     4501 tcaggctggc gcactagcgg gcgggcaaat tgtttcacaa gcagcaaaac gcagtgccga
     4561 tggcgagctg ctgttagcta cccgcaaccg cttaagcagc gttgtgccgc tgtcgcctga
     4621 tgcctggcaa atgttgagcg cgccgttacg ccagccgggc attgtggctt tgcgggaata
     4681 tttacgccag cgtccccccg cctgcatacg gcctcttaat caggtcgata acttatttat
     4741 tctgccggtc gctgagtgta tttcgctcgg ttgggatagc agccgccaga cgctggatgc
     4801 gcaggtaatc agcggcgaag gtgaagataa tctcctgacg ttatcccttc cggcatctgc
     4861 ctgttctcct tttgccgttg aacgcatggc ggcgcttttg caacaaacag acgaccccgt
     4921 gagtctggtt tctggctttg tcagctttgt agaggggcaa ttgacactgg aaccacgggt
     4981 gatgatgaca aaaacccgcg cctgggcgct ggacgcagaa actgcgcctg tggcaccgct
     5041 accttctgcc agcgttttgc ctgtgccgtc taccgctcat cagttgctga tgcgctgcca
     5101 ggcgttactt attcaactgc tccataacgg ctggcgctat caggaacaga gcgctattag
     5161 tcaggcagag ttgctggcga atgacctctc cgcggtcggt ttttatcggc tggcacatgt
     5221 gttgggacaa tttcgtaata cagaaagcga ggcacgggta gaagcaatga ataacggtgt
     5281 tttactttgc gaacaattat tccccatgct tcagcaacaa ggatgaaata gtgcttttta
     5341 ctaagagttc tactccagtt ccggactgct cacgccacgg tattaggcat atcctatata
     5401 gcccctggtg agagtcacca gttccttgat taaataaaat ggagttttac atgaaggctt
     5461 tcaataagct gttttccctc gttgttgcat ctgttctggt tttctctctt gctggctgcg
     5521 gtgacaaaga agaatcgaag aaattcagcg ccaatctgaa cggcactgaa attgccatta
     5581 cctatgtcta caaaggtgac aaggtgctta agcaatcttc tgaaaccaaa attcaatttg
     5641 cctccattgg tgcaaccacc aaagaagatg ctgccaagac acttgagccg ttaagcgcca
     5701 aatacaaaaa catcgcgggt gttgaagaaa aattaaccta taccgatacc tacgcgcagg
     5761 aaaacgtgac tatcgatatg gaaaaagtgg attttaaagc cctgcagggt atttcaggaa
     5821 tcaacgtttc tgctgaagat gccaaaaaag gtatcactat ggcgcaaatg gaactggtga
     5881 tgaaagccgc tggttttaaa gaagtgaaat aatctgtcgg cggccatgtt tcgcatggcc
     5941 gccatacccg tcttagcttt tcttcacatg ctggcgcgct gccagtccac gcagaaaata
     6001 acgtaaaaat tgatcgccgc attcgcggaa gtttttatga tccggtgcgc gcatcatcgc
     6061 tgtaatttcc ggcatcgaaa cgcggaactg ctgttcggtg aggatcgcca gaatgtcatc
     6121 ggttttcagc gaaaacgcga tgcgtaattt tttcagcacg atgttgttat taatgcgacg
     6181 ttccggctcc agtgccggag cagactcatc cttgccgcgt ttttcataaa tcaggccatt
     6241 gaggaatgac gacaaaacaa tgtccggaca acgctgaaaa ccctcttcgt cttctttacg
     6301 tagccagacg gcgatctgtt ccgcggtggc ttcgacatta cccagcgcca gaatacgcac
     6361 caggtcatta ttattggctt tcaaaatgta gcgcacgctg cgcagaatat cgttacttag
     6421 catgaggcct tcaggtgttg atgaggcaaa aagccatttt agcagtcttt tacaggccaa
     6481 tcgcctcttt taagcttttc agataacggc ggctgaccgg cacggttaag ccattacgca
     6541 aaatcaactc ggcctggccg ttatcttcca gacgaatctc ctgtaaatgc gcgaggttaa
     6601 ccagatactg acgatggcag cgcagtagtg gtgtacgact ttccagggta cgtaatgtca
     6661 attcggtaaa gccctctttc ccttcgtggc tggtaacgta gacaccgctc atccgactgc
     6721 tgacaaatgc cacatctttc atttgcagca aataaatccg actatgcccc gtacaaggga
     6781 taaatttcag cgcctgttga ttttccggta acagcgaaac atcctgcttg ctgcgctcct
     6841 gacgcaatcg cgccagcgtt ttctccagtc gcgcttcatc aattggcttc agcagataat
     6901 caaaggcatg ttcttcaaag gctttaatag cgtattcgtc aaacgcagtg agaaaaacaa
     6961 tatacgggcg atgttccggg tcaagcatcc ccaccatttc cagaccactg atgcgcggca
     7021 tctggatatc gagaaacagc acatccgggc gcagtttatg caccgcgccg atcccttcca
     7081 cggcgtttga acactctcca acgatttcaa tatcgctctg ctcctgcaaa aatacacgca
     7141 ggttctcccg tgctaacggt tcatcatcga caattaagac tttaatcatg cctcgtccct
     7201 ccatggtagt cgtaacgtta ttcgggtgta actatcaggc tcacaggcga cgcttattcc
     7261 atagtcatcg ccaaaccgtt cacgtaaacg cttatccacc agattcatcc ccagcccact
     7321 ggcattggtt accggttgat acaaaccggc attgtcttcg atctccagca tcaaatgttg
     7381 cccctcacgt cgggcgctga ttgccactcg ccctgtatcc agcagttgtg atgtcccatg
     7441 tttaatggcg ttttccacta tcggttgcag ggtaaacgcg ggcaattgct gctgggataa
     7501 ttcttgcgga atagcaatgt tgacctgcaa ccgcgactgg aagcgcgcct tttcaatttg
     7561 cagataagcg ttcacatgtt caatttcgtc ggcgagagta acaaactccg aaggccgctt
     7621 taagtttttg cggaaaaaag tggaaagata ctgcaccagc tggctggcct gttcgctgtc
     7681 gcggcggatc accgctttaa tggtgttaag cgcattaaac aaaaaatgag gattcacctg
     7741 ggcgtgaagc agtttgattt ctgactgggt gagcatcgct ttttgccgct catattgccc
     7801 ggcaagaatc tgcgccgaaa gcagttgcgc aatcccctcg cccagcgtgc ggtttattga
     7861 actgaataaa cggtttttgg cttcatacaa tttgatggtg cccatcacgc gctgattttc
     7921 gccacgcaac ggaattacca gcgtcgaccc cagtttgcat tgcggatgca aagagcaacg
     7981 ataaggtact tcgttgccat cagcgtagac cacttcaccg gtttcaatcg cttttaaggt
     8041 gtaaggcgaa gaaatcggtt tgccaggtaa atggtggtcg tcaccaattc cggtaaaggc
     8101 cagcaatttc tctcgatcgg taatcgcgac tgcaccaata tccagctcct gatacagcac
     8161 ctgagccact ttcatgctgt tcacttcgtt aaacccctgt cgcaaaatgc cttccgtcga
     8221 ggctgccact ttcagcgcag tggcagaaaa agccgaagtg tatttttcaa acatcgcgcg
     8281 tttatcgagc aatatacgca taaacagcgc cgcgccgacg gtattggtga ccatcattgg
     8341 cgcagcaata ttactcacca gacgcaccgc atcttcataa ggtcgggcga tcgccaggat
     8401 gatcagcatt tgcaccattt cagcgacgaa cgtgacggca ccggcggtaa tggggttaaa
     8461 gactttatca gtgcgcccgc gacggatcag gatgctgtgt accaggccgc cgagcaatcc
     8521 ttcaacgatg gttgagatca tgcaacttag cgcggtcatg ccccccatcg aatatcgatg
     8581 taagccgccg gtcagaccaa ccagcccacc gacgaccgga ccgccgagta agccgcccat
     8641 taccgcgcct atcgcacggg tattggcaat agaatcgtca atgtgcaacc caaaccaggt
     8701 gcccatgatg cagaagatgg aaaagacgat gtagcagaga aatttatgcg gcagacgaac
     8761 cgtgacctgc attaacggta tgaataatgg cgttttactc attaaccacg caatgactaa
     8821 aaaaacgcac atctgctgaa gcagcagcaa caccagatta aaatcgtaca tacccgcaaa
     8881 ccacacttcc ctttaaaacg cgtaacatac attgcctgcg tttaactttc tttgaactct
     8941 tgcagaaaaa tgagaattcg tgagtacgat cactcaaaat cgcctggcaa aaataaaatc
     9001 accctataga tgcacaaaaa acgggcaaaa ctacctggtt cgcaaaactg cgtctaaagt
     9061 taaaccggga cctcgcgagc aagggtgaga cgatggcgct ttacacaatt ggtgaagtgg
     9121 cgttgctttg tgatattaac cctgtcacgt tacgcgcgtg acgcaacatt tttaaaaatc
     9181 gacgaagatc gtccaggagc gccgtttttc aagggttgga tagatgattc gtttaccatg
     9241 tcgacgaact tcgacgaatt tcgccgataa aaagtgtccc atacatgccc cattgatgcc
     9301 ccataaacaa tatcaggaat tgacactggt tatccataca gataaaaata acacctgtac
     9361 ataaccacag ttacaggagg gtgttatgcg tgttgagatc tgtatcgcta aagaaaaaat
     9421 cactaaaatg ccaaacggtg ctgtggatgc gttaaaggaa gaattaaccc gacgcatcag
     9481 taaacgttat gacgatgtag aggtgatcgt aaaagccacc agcaacgatg gcctttctgt
     9541 tacacgcacc gcagataagg attctgcaaa aacatttgtt caggagactc tgaaagatac
     9601 ctgggagtct gctgacgagt gg
//
LOCUS       DAZPDU010000162         9499 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZPDU010000162 DAZPDU010000000
VERSION     DAZPDU010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9499
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.164"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..108
                     /locus_tag="ACHURS_003554"
     CDS             <1..108
                     /locus_tag="ACHURS_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR1239191.1"
                     /translation="HRTIIAFAVDDNKVFIAGVYHGGQIYETDFLSSAP"
     gene            complement(149..1231)
                     /locus_tag="ACHURS_003555"
     CDS             complement(149..1231)
                     /locus_tag="ACHURS_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BsuBI/PstI family type II restriction
                     endonuclease"
                     /protein_id="HFR1239192.1"
                     /translation="MNLPVVPPLNVIRERLQLIFPEGTENRGYLIREIAAKTIFVMFY
                     AGAVEGLGRWIRPSQVVSMGDSQAALTDDASRESWVKMTFSKQKIRPADAWYAENTRE
                     PIRDETIKNGLIPCNAIVERKGIPTTSSRPRYSLNRSFAALFDAGLEGDSLLSAISSW
                     QENHLNKAALARLRLLKSGAVIAADAVVVTFPNGEKRTLAPGPSSTIAKAVIEVFAPN
                     FLKNPTVLWLSESGNKVVAQDDVLAKALGLNIDASKALPDIILIDLGEDRTGSDMLVV
                     FTEVVATDGPINRERKVTLTHLAIDAGFSEKNLAFLTAFMDRSALPFKKAIPELAWGS
                     YAWFASEPEHLIDLRDGYPVKISQRK"
     gene            complement(1228..3060)
                     /locus_tag="ACHURS_003556"
     CDS             complement(1228..3060)
                     /locus_tag="ACHURS_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFR1239193.1"
                     /translation="MARMFDTLGFYIMNVQHFDFFTDNEFSNLERYQTCKAMAKGYAG
                     NYKTGQSRLMHARSYCFSIVAAYWDTLAKKQKSSIKMRAVPHTVYIEDIPCDARELAE
                     RTGELIAQFPAEDAGYLIGSIYTVMLPSAYRSELGAYYTPPPLVARLLDLAEKSGVDF
                     SHASVIDPACGGGAFLAPVAIRMLKKDKGSSPEWMLRRISRRLKGIEIDPFAAWMSLV
                     LLESVLMPLCVKVKRRLPEDTIIVADALQQDKFSGYDLVVGNPPYGRVTLDIKTREKY
                     SRSLFGHANLYGLFTDLAVRMVKEKTGVIAFLTPTSFLGGQYFTALRTLLTEKTTPYA
                     LDFVTDRDGVFDDVLQETMLTAFKAGEHKVRAQVSSLVPKGLNKAKIEKIGNVDIEKG
                     GATWLLPRVKDDACFLNVLKQMSTRLADLGYSVSTGQLVWNRFKSQLRTTKGKNSYPL
                     VWAESITSAGFRFSADRKNHVPYIDITPQQGFLVTKSECVLVQRTTSKEQDRRILAAI
                     LPQGFIDETGGVVVENHINIVYSNGLFSAVRPDVIDMLLNSHVVDRAFRCISGSVAVS
                     AYELNSIPLPSLEQVMEIQSLIDAGVHRRIIERTIAGFYGVMIA"
     gene            complement(3275..3631)
                     /locus_tag="ACHURS_003557"
     CDS             complement(3275..3631)
                     /locus_tag="ACHURS_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239194.1"
                     /translation="MEIKPEDELSNIVLFPVKEDNPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEERERRKNIEKLIQIE
                     RNAKARIRRVTKYSPE"
     gene            complement(3689..4111)
                     /locus_tag="ACHURS_003558"
     CDS             complement(3689..4111)
                     /locus_tag="ACHURS_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR1239195.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLIS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPSVVDPDLIWSLPDG
                     EIRRYDRHQNIICRECRKSEVMQRVPAFYQGDFQEAVL"
     gene            complement(4127..4852)
                     /locus_tag="ACHURS_003559"
     CDS             complement(4127..4852)
                     /locus_tag="ACHURS_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFR1239196.1"
                     /translation="METVFDALKALKRASSHVVASRLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPKAASIAETDGKVFPQPAGVALPVQEAA
                     TQEEIKTETVADIVQSLPSFTETQADDLVLPSLHMANRELRRAKSHVQKWERVCAALR
                     ELNKHRDIVRQIVDSSGRIVSEK"
     gene            complement(4874..5620)
                     /locus_tag="ACHURS_003560"
     CDS             complement(4874..5620)
                     /locus_tag="ACHURS_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR1239197.1"
                     /translation="MKNIAAAGVLERIHRLAPQGAVPPYRTVEEWREWQLAEGRKRSE
                     EINRQNRQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMAKGRSVIIVTESDVMSVLHDSYDNGKSGEKFLQE
                     LCGVDLLVLDEIGIQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGCRWVTFNWDSWRSNVSFPGVVK"
     gene            complement(5627..6691)
                     /locus_tag="ACHURS_003561"
     CDS             complement(5627..6691)
                     /locus_tag="ACHURS_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HFR1239198.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGASRNVTHMSRRVTTDTDKDTDKEDQNTM
                     VHGVKNATNQAGDVQTVNPGQPAGSTPEADSAYALKADSGAVQQVMTARPEQSLQLQQ
                     PEADSAIQREADRVAPENTGQPVGRVDYPDVFEQVWREYPLRAGANPKKSAFSAWKAR
                     LREGVPPETMLDGVRRYARYLAATGKAGTEFVQRATTFFGPDRNFENPWLLPVSGTNN
                     QRCVNHISEPDTEIPPGFRG"
     gene            complement(6763..7188)
                     /locus_tag="ACHURS_003562"
     CDS             complement(6763..7188)
                     /locus_tag="ACHURS_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFR1239199.1"
                     /translation="MKIKHEHIRMAMKAWAHPDGEKVPAAEITRVYFELGMTFPVLHN
                     DSKHNTLYLNTQKIFRWLDKDTPDAVEKIQALLPAIERAMPPPLVARMRSHSSAYFRE
                     LVETHERLVRDADDFVAVAIAGFNQMNRGGPAGNAVAVH"
     gene            complement(7185..7448)
                     /locus_tag="ACHURS_003563"
     CDS             complement(7185..7448)
                     /locus_tag="ACHURS_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1239200.1"
                     /translation="MNLGEYLHHFHITQSSFAEIVGASQGMVSHVITGRAKLTGEKIL
                     RWCEATGWVVTPHEIDSKTYPNPTDGLPAEFQANTQPSAGVDS"
     gene            7560..8060
                     /locus_tag="ACHURS_003564"
     CDS             7560..8060
                     /locus_tag="ACHURS_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1239201.1"
                     /translation="MKRKSLSDTDLNAARRLKEIWMAKKSQLGLTQERAAEIMGFSTQ
                     GAVSHYLNGQTPLNLEAVLKFAGLLKVPPESIRPDMVDLLQIARMYCREPQQDNIVAL
                     PADTEQTEDELPFAVEPMERDLIHTFRAFPKEDQEHMLQEMKDKKESMDRTVARWLAA
                     QKGRRA"
     gene            8080..8274
                     /locus_tag="ACHURS_003565"
     CDS             8080..8274
                     /locus_tag="ACHURS_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFR1239202.1"
                     /translation="MSTVLSPMVSEFETVEQENSYNEWLRAKVAASLADPRPAIPHDE
                     VMAEMEQIIAQIAAATHRGE"
     gene            8274..8564
                     /locus_tag="ACHURS_003566"
     CDS             8274..8564
                     /locus_tag="ACHURS_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR1239203.1"
                     /translation="MLPVLWLPSARDDLRQIVAYIAKENPAAARRMKIRIETSVLPLT
                     EHPYLYPPSERVLGLREIVTHPNYIILYRVTASSIEVVNIVHSRRQYPGKNS"
     gene            8843..8995
                     /locus_tag="ACHURS_003567"
     CDS             8843..8995
                     /locus_tag="ACHURS_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFR1239204.1"
                     /translation="MDTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTESGARCW
                     LARNTD"
     gene            9007..9372
                     /locus_tag="ACHURS_003568"
     CDS             9007..9372
                     /locus_tag="ACHURS_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001320327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239205.1"
                     /translation="MEFKDLPSDVQKTAAHTLHSVLREIGKDIASEPAKDLARKIKTA
                     FVELYNVGTDSETVETKTVSSPIFSLEPSVLSGEICTEISSELLPVIREAIRRRGLDG
                     SYDHDVLQVLRTMVTSLGI"
BASE COUNT         2442 a         2490 c         2017 g         2550 t
ORIGIN      
        1 cacagaacaa ttattgcttt tgccgttgat gacaataaag tttttatcgc aggcgtatac
       61 cacggtgggc aaatctatga aaccgatttt ttatccagtg caccgtaaaa cccccatcct
      121 tcagggcgtg gcggatatca aactttggtc attttctctg gctgatcttt accggataac
      181 catcccgaag atcaattaag tgctccggtt cagaagcaaa ccatgcataa gatccccacg
      241 ccaattctgg tattgccttt ttaaaaggca gagcacttct gtccataaat gccgtaagga
      301 atgcaagatt tttctcgctg aacccagcgt caatagccaa atgagtgaga gtaacttttc
      361 gttccctgtt tataggccca tctgtcgcaa ctacttcagt gaaaactaca agcatgtccg
      421 aacccgttcg gtcctctccc aaatcaatca aaataatatc tggaagtgct tttgaggcat
      481 caatattcag ccccaatgct tttgccaata catcatcctg agcaacaact ttattcccgg
      541 attcagacaa ccagagcaca gttggattct taagaaaatt aggagcaaaa acttcgatga
      601 cggccttagc tattgtactt gacgggcccg gagctaaagt tctcttctct ccatttggga
      661 aagtgacgac tacggcatca gcagcaatca ctgcccctga tttcaataaa cgcaacctag
      721 caagagcggc tttgttcaaa tgattttctt gccaggaact tattgcactc agcagcgaat
      781 ctccttcaag cccggcatca aagagtgctg caaaagagcg attcaaactg taacgaggac
      841 gagaggatgt ggtgggtata cctttacgct caacaattgc attgcaaggt attaggccat
      901 tttttatagt ttcatcacgt attggctcac gcgtattttc cgcataccat gcatcagcag
      961 gccttatctt ttgtttagag aaagtcatct ttacccagct ttctctggac gcatcatcag
     1021 taagagcagc ctgcgaatct cccatactta caacctggct gggccttatc catcgcccca
     1081 gcccctcaac cgcaccagca taaaacatga caaaaattgt ttttgcagct atttcccgaa
     1141 ttaaatatcc acgattctca gtcccttcag ggaaaattaa ttgtaatctt tcccggataa
     1201 cattcaatgg gggaacaaca ggcagattca tgcaatcatc actccataga aaccagcgat
     1261 agttctctca ataatccgtc tgtggacgcc agcatcaatt aacgattgaa tctccatcac
     1321 ctgctccaga gaaggcaacg ggattgaatt caattcatag gccgaaacag caacacttcc
     1381 gcttatacac ctgaacgccc gatcgacaac atgcgaatta agtagcatat caataacatc
     1441 agggcgaacc gcagagaaca atccgtttga atatacaata ttaatatggt tttcaactac
     1501 tacgccacca gtttcatcta taaatccctg aggtaaaatt gccgctaaaa tccttctgtc
     1561 ctgttccttc gatgttgtcc gctgaacaag cacacattca gattttgtca caaggaaacc
     1621 ctgttgtggg gttatatcga tatagggaac atgatttttt ctgtctgcac taaaacgaaa
     1681 accagctgaa gttattgatt ccgcccagac taatgggtag ctattttttc ctttcgtcgt
     1741 acgcaactgc gacttaaatc gattccagac taactgacct gtagagactg agtatcccag
     1801 atcagctaaa cgcgtagaca tctgttttaa tacattaaga aaacaggcat catctttaac
     1861 gcgtggcaaa agccacgttg ctccaccttt ttctatatca acattaccta ttttctcaat
     1921 ttttgctttg ttcaacccct tagggacaag tgaggatacc tgggcccgaa ctttatgttc
     1981 tcccgccttg aatgctgtca acattgtttc ctggagaaca tcgtcaaaga ccccatccct
     2041 gtcagtgacg aaatcaaggg catacggagt agttttttcg gtcaaaaggg ttcgcagcgc
     2101 ggtgaaatac tgcccaccaa gaaaagaagt tggcgtaaga aatgcaataa ctcctgtttt
     2161 ctctttaacc atgcgaacag ctaagtcggt gaacaatcca tatagatttg catgaccaaa
     2221 taacgaacga gaatattttt ctctcgtttt aatatcgaga gtgacacgtc cgtaaggcgg
     2281 attccctacc acaagatcat aaccagaaaa tttatcttgc tgaagcgcat cagcaacaat
     2341 aattgtatct tcaggcaatc tacgttttac ctttacacat agtggcatta gtacagattc
     2401 gagcaatacc aaactcatcc aagccgcaaa tggatcgatt tctatcccct ttaaacgacg
     2461 actgattctc ctcaacatcc attcagggga agaccccttg tcttttttta acattcgaat
     2521 ggctactggt gccaaaaatg caccgccacc acacgcagga tcaataaccg atgcatgaga
     2581 aaaatcaaca ccagactttt cagccaggtc caagagacgc gctaccaaag gtgggggggt
     2641 atagtaagcc cccaattccg aacggtaagc ggatggcagc attaccgtat aaattgaccc
     2701 aatcagatat ccagcgtcct ccgccggaaa ttgagcaata agctcacctg ttcgttcagc
     2761 gagctcgcgt gcatcacatg gaatgtcttc tatgtaaaca gtgtgcggta ctgccctcat
     2821 tttaatgctg cttttttgct tttttgcaag ggtatcccaa tatgcagcta cgatgctgaa
     2881 gcagtatgag cgggcatgca tcaaccttga ctgcccggtt ttgtaattgc ccgcataacc
     2941 tttggccatg gctttacaag tttgatatct ttccagatta gaaaactcat tatctgtaaa
     3001 aaaatcaaaa tgttgcacat tcataatgta aaatcctaat gtatcaaaca tgcgggccat
     3061 aacatctcat ggtggcgcat agcaaactgg cccgaagaat atcacgcacc aagtcgatct
     3121 cataacccgt tatgacaata aatctttgta gctgaactca cttggtgatt tgtcaatcat
     3181 tcctggcctc cagttcgtcc tgaatctctt tatcgagttc accgtatttg ttagtcctca
     3241 tcactcacga cttgacttcc agaaataacc ttgattattc ggggctatat tttgtcaccc
     3301 tgcgtatccg cgctttcgca ttacgctcaa tctggattag cttttctata ttttttcgcc
     3361 tttcccgctc ctcctggcgc aagagcctta catcatctgc cagtctggtt tctcttttcg
     3421 ccacagagag catccagtca aatggctcca caactgcacc gcaaatttta cagcggacct
     3481 gacgctcttt ttcgtcaacc cggacagaag cgtgatggca atatggtctt tccgatggct
     3541 cataaagaaa attaacctga ttacgagggt tatcctcttt taccggaaat aaaacaatat
     3601 tgcttaactc atcttctggt tttatttcca tgctcctctc ctttgatgcg aatgccagcg
     3661 gtaattgaag cctgatagct aatttcactc acagtaccgc ctcctgaaaa tcaccctgat
     3721 agaaagccgg tacacgctgc ataacttcac tcttccggca ctcgcgacag attatgttct
     3781 gatgtctgtc gtagcggcgt atttctccgt ctggtaatga ccagataagg tccggatcaa
     3841 ccacagatgg tttcttcagc tttgccctcg agagtttttt gcgggcgttt tgccagtcct
     3901 tacgagcctg ttcagacggg aataacccgt agccagagtt gtatacatcc ccactggcaa
     3961 ccagctctct ggcgagaacg cttattaaat atcttgtcgc cccggtttta gcttccagtt
     4021 gtcgtaacgt ctcgcgccca ctctggcgta cgagttcaac aacctgcccc ttaatttttt
     4081 cccgctcttc ctgtgtaaat acttttgcca taagcgcccc cggcaatcac ttttccgaca
     4141 caatacgacc ggaggaatcg acaatctgtc gaacaatatc ccggtgcttg ttcagctccc
     4201 gcaacgcggc gcagacacgc tcccacttct ggacatgact tttcgcccga cgcagttcgc
     4261 ggtttgccat atgcagcgat ggtaaaacca ggtcatccgc ttgcgtttcg gtgaacgatg
     4321 gcaacgactg cacaatgtcc gccacagttt ctgttttaat ttcttcctgt gttgcagctt
     4381 cctgtaccgg taacgcaaca cctgctggct gaggaaagac tttaccatcc gtttccgcta
     4441 tggatgcggc tttcggctct gccggtaaat cagcgcccgg tatgcagtac cgaaatttac
     4501 cgccctgatt cacgcgaatc agacgccctt tgctgattgc catggccagc gatgaattcg
     4561 cccggcggga ggtaatcccg aacatcagtg ccagctcatc cgccgtttgt gggccatgtt
     4621 gttcaatcgc ctcagtcagc atttgcgctg tcactttcgg taccggtgac actggttcac
     4681 tttcaccagc ctgaatcagc caccacatcg aacccttgtt atccgcttca ccacggcgct
     4741 tcagtttcca cagttcgttg accgcatctt cacggctgat tccaaggcgg gacgccacca
     4801 catgtgaaga ggctcttttc agtgctttca gtgcgtcaaa tacagtttcc attaaaacat
     4861 cctccaacaa aaattacttc acaactcccg gaaagctgac atttgaacgc cagctatccc
     4921 agttaaacgt cacccaccga caaccgttca tggtcatgcg gtccataatc ctctcaccaa
     4981 gaagcgtgct cattgcggca tgattcaggt ttgttaacat cccgacactg cacagtgatg
     5041 ctgtccggcg atcaattatc tggtgcaata ccacctgctc gtttttcgtc tcccgctgaa
     5101 tgcctatttc atccaggacc agcaaatcaa ccccgcaaag ctcctgtaaa aatttttcac
     5161 cggatttgcc gttgtcgtag ctgtcatgca acacgctcat gacatcagac tcggtgacga
     5221 taatcacgct gcgccccttc gccatcagcc ggttacccat cgccgctgca aggtgatttt
     5281 tcccggtgcc ggttttaccg ctgaacacaa aattcgtgca cccggtcatc agttcgtcag
     5341 ctatggattt ggcctggctc agcgcgtatt tttgcccgtc gttctgcacc tgataattcg
     5401 caaacgagca tttgctgtgc agaggctgga tgcccgaacg attcaggatt ttttccaccc
     5461 gcaactggcg attctggcgg ttaatctcct cgctgcgttt tcgtccttca gcaagttgcc
     5521 attcccgcca ctcctccacc gtccggtacg gtggaaccgc cccctgtggt gcaagtctgt
     5581 gaatacgttc aagaaccccg gctgccgcaa tgtttttcat gccacatcac cccctgaatc
     5641 ccggcggaat ttcggtatcc ggttcagaaa tatgattcac acaacgctgg ttgttcgtgc
     5701 cgcttaccgg gagcaaccag gggttttcaa aattccggtc cggcccaaaa aacgtcgtcg
     5761 ctcgctgaac aaattccgtt cccgctttcc cggtcgccgc caggtatctc gcgtaacgcc
     5821 tcacaccatc cagcatggtc tctggtggca ccccctcgcg caatctggcc ttccaggcac
     5881 tgaatgcgga tttcttcggg tttgccccgg cacgcaacgg gtattcccgc cagacctgtt
     5941 cgaacacatc cggataatcc actcgtccca caggctgccc ggtgttttcc ggggctaccc
     6001 gatcggcttc ccgctgaatg gcggaatcgg cttcaggctg ctgcagttgg agtgattgct
     6061 ccggccttgc ggtcatcacc tgctgcacag cgcccgaatc ggctttcagc gcatacgctg
     6121 aatcggcttc cggtgtcgag cctgctggct gaccaggatt gacggtctga acatcccctg
     6181 cctggttcgt ggcgtttttt acgccatgga ccatagtgtt ttgatcttct ttatctgtat
     6241 ctttatctgt atctgtcgtg actcgtcgtg acatgtgcgt gacatttcgt gacgcgccgt
     6301 gacaatcgcc attttgttcc cgctttcttt ccctctctcg ctgcgccctc ttgcgctctg
     6361 caggagattt tgcggtttgc gaaatattgc cgttgtcctc tttaagcacc tggcgttttt
     6421 cccatccagt gattaaatca ccatcaagta cccgcccctg catcgtctgc aaaattgaat
     6481 caattacctc ttctgtcacg tcgagcgcac ttgccaaatc ttctgtcgtg acatcaatgt
     6541 gacctcgcgt gacatttcgt gacgcgctca ccaggaggtg gatatacact gccatcactg
     6601 ttgcaattgg ctgccctgac accctggcaa ttgttcgcca cttagggtca tttggcatgt
     6661 catgccataa tctgagccag gcgttagcca tactcacctc ttctgatacc gaatcttttt
     6721 actcacgagt tgccgggagc gattcgatat ggctattgtc agtcaatgta ctgccacagc
     6781 atttcctgcc gggccaccac ggttcatctg attgaaaccg gcgattgcca ctgcgacaaa
     6841 atcatcagcg tctctcacca gtcgttcatg cgtctccacc agttcccgaa aataagctga
     6901 actgtggctg cgcattcgag ccaccagcgg aggtggcatt gccctttcga tcgcaggtaa
     6961 caacgcctga attttttcaa cagcgtcagg ggtgtcttta tccagccaac ggaaaatctt
     7021 ttgggtgttg agataaagcg tgttgtgctt gctgtcgtta tgcagaaccg gaaacgtcat
     7081 tcccagttcg aaataaaccc tggttatttc agctgccgga actttttcac cgtccggatg
     7141 cgcccaggct ttcatcgcca tgcggatgtg ttcatgcttg attttcatga atcaaccccc
     7201 gccgatggtt gtgtgttagc ctgaaactca gcaggcaagc cgtcagttgg gttggggtag
     7261 gttttactgt caatctcatg tggggtcacc acccatcccg ttgcttcaca ccaacgtaat
     7321 attttttctc ctgtaagttt tgcacgcccc gttataacgt ggctgaccat cccttgggat
     7381 gcgccaacaa tctcagcaaa tgaactctgg gttatatgaa aatgatgcaa atattctcca
     7441 agattcataa tccgccctat gtgatgtgtt accaatgcat aattaatagc actgttattt
     7501 ttaaaagtaa atagtgatgc tatttcaaag tgattagcaa tcttattaga attggatgta
     7561 tgaaaagaaa atcattgtca gacaccgacc tcaacgccgc cagaagactg aaagaaatct
     7621 ggatggcaaa gaaatctcag ttgggattga ctcaggaacg tgcggcggaa attatggggt
     7681 tttcaacaca aggggcggta agtcactacc taaatggtca aaccccacta aaccttgagg
     7741 ctgttctaaa gtttgctggc ttactgaaag ttccccctga gtcaatcagg ccagacatgg
     7801 tagatttgct gcaaattgca aggatgtatt gtcgagaacc acagcaagat aacatagtcg
     7861 cattaccagc agacacagaa caaacagagg atgaactacc gtttgccgta gagccgatgg
     7921 aacgagattt aatccatacg ttcagagctt ttccgaaaga agatcaagaa cacatgcttc
     7981 aggagatgaa agataaaaaa gaatcaatgg atcgtacggt tgcgcggtgg ttagctgcac
     8041 aaaaaggccg tcgcgcctaa atcagaggag atatgtaaaa tgagtacagt cctttcccca
     8101 atggtttcag aatttgagac agttgagcaa gaaaatagtt acaacgaatg gctgcgggcc
     8161 aaagtagcag caagcctcgc ggatccccgc ccggcgattc cgcatgatga ggtaatggcg
     8221 gaaatggaac aaattattgc ccaaattgct gctgcaactc acagaggtga ataatgttac
     8281 ccgttttatg gctaccatct gctcgtgatg atttgcgcca gatcgtagcc tacatagcta
     8341 aagaaaatcc cgcggcagca cgcagaatga aaattcgaat tgaaacatcc gttttgccgc
     8401 tcacagagca tccttacctg tatcctccaa gcgagcgagt tcttggtctt cgtgaaattg
     8461 taactcatcc taactacatc atcctttacc gagtaacagc atcaagcata gaagtagtaa
     8521 atattgtgca ttctcgaaga cagtatccag gcaaaaacag ttaattattc ccgccgacag
     8581 aaccacctta gggtggtttt ttcttgcccc aacaaaaaac aatagcgatg ctatttacat
     8641 caaaaaaata atgatgctat tgtttcaggc gtcaacccac cccgccccac agaatgcagg
     8701 gcaatacttc gagttaccag acagtggtca ggggttaagt agccagcccg aggcgtaaga
     8761 acatgacggc agggttcaat ctttaactat gcagcaggtt ttttgttccg ccaccccggc
     8821 gttaagggga aatgaggtca gcatggatac tatcgatctt ggcaacaacg aatctctggt
     8881 gtgcggcgtg ttccctaacc aggatggaac gtttaccgcc atgacgtata ccaaaagcaa
     8941 aacgtttaaa accgaatctg gcgcgcgttg ctggttagcc agaaacactg actgatgagg
     9001 ctgacgatgg aatttaaaga tttaccgtct gatgtacaga aaacagcagc tcatacattg
     9061 cattctgtgc tgcgagaaat cgggaaagat attgcaagcg agccagcaaa agatctggcc
     9121 cggaaaatca agaccgcttt cgttgagctt tataatgttg gcactgactc tgaaactgtc
     9181 gagaccaaga ccgtaagttc accaatattc tcacttgagc caagcgtatt atcaggtgaa
     9241 atatgcaccg agatttccag cgaactgctt ccggtaatac gagaagcaat tcgccgtcgc
     9301 ggtttggatg gaagttatga tcatgacgtt ctgcaagttc tcaggacaat ggtgacttca
     9361 ctggggattt gatccctgcg tcctctaagc ttttgatata gcggtcctcc aagaagcggt
     9421 aaattttgtc gaaatcatct tttccctctg gtttattcag aggattacag gacccgtaag
     9481 tagcggcata catttcaac
//
LOCUS       DAZPDU010000163         9496 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZPDU010000163 DAZPDU010000000
VERSION     DAZPDU010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9496
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.165"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            122..952
                     /locus_tag="ACHURS_003569"
     CDS             122..952
                     /locus_tag="ACHURS_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418745.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfZ/YjiC family protein"
                     /protein_id="HFR1239206.1"
                     /translation="MSMPLSNALQSQIITDNHFLHHPIVDSEFTHKLKYARMDLENIY
                     LPPLTRGNNHNYDGKSAVEIRKLDISKESWPFNYVTGACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIMTVTGVANSSGFALAALLNANIELGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDENAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1093..1866)
                     /gene="uxuR"
                     /locus_tag="ACHURS_003570"
     CDS             complement(1093..1866)
                     /gene="uxuR"
                     /locus_tag="ACHURS_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFR1239207.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWRHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2081..3541)
                     /locus_tag="ACHURS_003571"
     CDS             complement(2081..3541)
                     /locus_tag="ACHURS_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFR1239208.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3622..4806)
                     /gene="uxuA"
                     /locus_tag="ACHURS_003572"
     CDS             complement(3622..4806)
                     /gene="uxuA"
                     /locus_tag="ACHURS_003572"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFR1239209.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5146..6489
                     /gene="gntP"
                     /locus_tag="ACHURS_003573"
     CDS             5146..6489
                     /gene="gntP"
                     /locus_tag="ACHURS_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFR1239210.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDTATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGQLELVNSVVGLIIVLIISMVA"
     gene            complement(6653..7555)
                     /gene="fimH"
                     /locus_tag="ACHURS_003574"
     CDS             complement(6653..7555)
                     /gene="fimH"
                     /locus_tag="ACHURS_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFR1239211.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7575..8078)
                     /gene="fimG"
                     /locus_tag="ACHURS_003575"
     CDS             complement(7575..8078)
                     /gene="fimG"
                     /locus_tag="ACHURS_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HFR1239212.1"
                     /translation="MKWRKRGYLLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNSGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8091..8621)
                     /gene="fimF"
                     /locus_tag="ACHURS_003576"
     CDS             complement(8091..8621)
                     /gene="fimF"
                     /locus_tag="ACHURS_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFR1239213.1"
                     /translation="MRNKPFYLLCAFLWLAVSRALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSALSWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8631..>9496)
                     /gene="fimD"
                     /locus_tag="ACHURS_003577"
     CDS             complement(8631..>9496)
                     /gene="fimD"
                     /locus_tag="ACHURS_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFR1239214.1"
                     /translation="KSYCLNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLA
                     GVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQL
                     YYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYAT
                     EYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPL
                     PFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQ
                     QLLTQLSAECR"
BASE COUNT         2259 a         2484 c         2376 g         2377 t
ORIGIN      
        1 gtgtagagtt gatatcacta caacagaaac atgcatgtca tttgtttact tctcttttta
       61 ctacagaaaa atttatctat ttttctaaac atcagtgaaa tactcataaa aggtaactat
      121 tatgtctatg ccattaagca atgcccttca gagtcaaatt attacagata atcacttcct
      181 tcatcaccca atagtcgaca gtgagtttac acataaatta aaatatgcac gcatggattt
      241 ggaaaacatc tatcttccgc ctcttactcg tggcaataac cataattacg atggaaaaag
      301 cgctgtcgaa attagaaaac ttgatattag taaagagtcc tggccattta attatgtgac
      361 aggagcatgc agagaatccg atggtattac aacaacagga cgtatgctct atcggaactt
      421 gaaaatcacc tcagctttag acgaaatata tggtggcata tgtaaaaagg cgcatgctgc
      481 cacagagtta gcagaaggtt tacggctaaa tttatttatg aagagccctt tcgatcctgt
      541 tgaggactat acagttcatg aaataacatt aggccctggt tgcaatgttc ctggttatgc
      601 cggaaccacg ataggatata tttcaaccct tccagcttct caggcaaaaa gatggaccaa
      661 tgagcagcca aggattgata tctatattga tcagataatg acggtaacag gggtagcaaa
      721 ttcttccgga ttcgcccttg ccgcattact taatgctaat attgagttgg gtaatgaccc
      781 aataattggt atagaagcct atcccggtac cgcggagatc catgcaaaaa tgggatataa
      841 agttatcccg ggtgatgaaa atgcaccact caaacgtatg actctgcaac cgtcatctct
      901 tccagaactg tttgaattaa aaaatggtga gtggaattat attggtaaat aactaccagg
      961 aagaatgagt actaacattc aggttataca gatagctaaa caataccctg caatttacaa
     1021 agtaatactc attgcgactt cttaatttaa gaaagcgcaa ggaacattta cccttgcgct
     1081 tattataata agtcaggcgt caactttatc cagcggccat gaatcaaaca gatagccatc
     1141 aaaataaata tcgtcaacgt tcgagaattc cagcagacgt tgcttaacgt tttccagatg
     1201 ccgccacatt gccagcttcg ccgctcgggc atcttttttg attaacgcgg cgaggatctg
     1261 tttgtgatcg cccaaccact ctttgcgata caggctgtca tccagatggc tgtgcaactg
     1321 gatccacatt ggattgtttt cccgccactg ccaggactga cggaacagct ccaccagcat
     1381 gctgttatgc gttgcttcgg caatggcgag atggaactgc atatcaccac tttcgctgct
     1441 gcccggcgcg ctggaagcca gctcacgctc ttccagttgc aatgcctgac gcattttgac
     1501 gatatcttcg cgggtagcct gcaaagcggc aaattccgcg atgttacttt ccagtaactg
     1561 acgcgcctgt aacagctcaa aaggaccggc atcattgcag acgttagcat ccggactgtc
     1621 tgtgttgtgg ctgcctgagc tatcaagaac atagataccg gcaccccggc gcacttccac
     1681 cagccctttg atctccagca tgatcagcgc ttcacgtacc accgtcctcg tgacatcaag
     1741 catttctgca atttcacgct ccggcggcag ccgttcgcca ggattgtatg gcgtctttat
     1801 gatcagatcg cggatcatcg ccccgacttc ctggtaaggt ctttgcgcag aggtggcaga
     1861 tttcatggtg ttgtgtccgt taggtgcggt taatcaaatt tttgccggat gcggcgtgaa
     1921 cgccttatcc ggcctacaac gggcaatttt acggcacccg ttaatctcaa actataccat
     1981 tagcactgga agttggcacg tacaggtggg caaaaaaacg aaaactggtc aaccaaatat
     2041 cagtttgatt gaccagtaag tctgtagggt aatcagttag ttacagcgca gccacacact
     2101 cgcgcgcacc gcgttcgcac agctgctgat atgccgccgt caccgcgcca acaaagtcgg
     2161 cattctgcgg cagatcatcg gcaaaaatgc cgctcaggcc cagcagcgct ttcacgcggt
     2221 ctgcgccctg atactgcgcg ttaatcttct ggaactcggc cagcatcggg tcaaccacgt
     2281 caatggcatt accctgttca tccacgccct gggtgtagcg catccagcca gccacgccca
     2341 gtgccaggtg acgccagctg ccgccgtttt gcaggtgcag acgcaccgga tccagaagac
     2401 gctgcggtaa cttctggctg ccgtccattg caatctgcca ggtacggtga cgcagagacg
     2461 ggttgctgaa acgctcgatc agcagcgtcg cataggcgtt caggtctgta ccttccggca
     2521 tcgacagcgt tggcgcttgt tcctgcatca tcagggcaaa ggccgctttg cgataatccg
     2581 gattagtcat ggtgtcggca atggtttcat agccgccgag gtaaccgagg tacgccagga
     2641 aagagtggct gccgttcagc atacgcagtt tcatcatttc gaacggcaca acgtctgcaa
     2701 cgaactgtgc gcccacttta tcccagtccg gacgaccatt aacgaagtta tcttcaatca
     2761 cccactgacg gaacggttcg caggcaatgg cgcacgggtc gtaaacaccc agctggtcag
     2821 caatttcctg taaggtttct ggcgtcgccg ccggaacgat gcggtcaacc atggtgcacg
     2881 ggaaggtgac gttctcttca atccatgccg ccagctgcgg atcacgtgcc tgagccagcc
     2941 ccagaaccgc gaccttcgcc acatgaccgt tttcacgcac gttgtcgcag gacatcaccg
     3001 taaacgcttt cagccctttt tcacgacgca gacgcaaggc ttcgacgatg taaccaatcg
     3061 cggacttcgg cgcagtcggg ttttccagat cgtgcttgat cagcgggtta ttgagatcca
     3121 gctgaccgct tgccgcatcg gcgcaataac ctttctctgt gaccgtcagg gagacaatcg
     3181 ccgtctgcgg acgcgccatc gcgttgagaa taccttcaca accgtcgatt tccggatgca
     3241 gggcttcttt catcgaaccg ataattttca gctcggtgct ttctgcgcct ttttccgcca
     3301 cggtgtacag cagttgctgt tttttcaggt tttcaatcag cacacggtcg ttgcctggca
     3361 tcaggttaac ttcgcagata ccccagtcgc tgtcggtact ttccagcaga tgatgggtgt
     3421 acagcgcctg gtgcgcgcgg tgaaacgccc cgcaaccgag atgcacaatg cgtgattcca
     3481 gacgagaatg atcccatgac gggcgggcaa ccggcagatt gctgtcaaca atagtagtca
     3541 tcgtaaactc cagaatccgt cttcagagag tgacggcaag gaagaaggaa ccggcacggc
     3601 gcgcagccat gccggtgggt atcaacggct aaagaaagcg cgctggatcg ccagttcgac
     3661 accgcgaact tcagccaggc ctttcagacg accaattgcg gagtaacctg ggttggtttt
     3721 cttcttcagg tcgtccagca tctgatgacc gtggtccgga cgcatcggga tcaggtcttc
     3781 tttgccttcc gctttacgac ggtgttcttc ttcaacaatc gctttcacca cttcgtacat
     3841 atcaacgtca ccgttcaggt gcgccgcttc gtggaaggtt ttcgggttat cttcacgcat
     3901 ggtggagcgc agatgggtga agtaaatacg cggaccgaac tgcttgatca tatcaaccag
     3961 atcgttgtca gcacgcacgc cgtaggaacc tgtgcacatg gtgaaaccgt tcgccatgct
     4021 gtttacggta tcaaccatcc actgcatatc ttcaatggtg gaaacaatgc gcggcaggcc
     4081 gaggatcggg cgcggcggat cgtccgggtg aacagccata cggacgccaa cttcttcggc
     4141 aaccggaata atcgctttca ggaagacggc aaagttttcg cgcagtttgg ctttgtcgat
     4201 atctttatac agctccaggt gcttacggaa ctggtcgagg gtataccctt cttccgcgcc
     4261 cggcagaccg gcaatgatgt tacgggtcag acgcgctttg tcttcgtcgc tcatagtggc
     4321 gaagcgttca gcggcctgag caatttcttc ttcggtgtaa tccgcttccg cgcctggacg
     4381 cttcaggata tgcatttcga atgcagcgaa ttcgatctgg tcgaagcgca gagctttgga
     4441 gccgtctggc agcacgtatt cgaggtcagt acgggtccag tcgagcaccg gcatgaagtt
     4501 gtagcacacg gtgcgaatgc cgcactgcgc caggttgcgc agagtctgct gatagttagc
     4561 gatccactgt tcatagttgc cagtgtgggt tttgatatct tcgtgaattg gcacgctttc
     4621 tacgacagac cataccaggc ctgcgtcttc aacgatcgcc ttgcgtttga ggatctcttc
     4681 tacggaccat acttcgccgt tcgggatatg gtgcagcgcg gtaaccacgc cagttgcgcc
     4741 cgcctgacgg acatcagcta gagaaaccgg atcgtttggg ccgtaccagc gccaggtctg
     4801 ttccatgatt tttcctcttt cacatagtgg gttccacagc aaagagacgt ggagccccgg
     4861 agttgtttag gtatccggat gaacaaccag accgcagccg tggcgttgca tcggatgaga
     4921 tggcgtataa gttctacctt ttggtaagac atgtcaaccc gaaatctgaa attggttaac
     4981 cacatcgcaa gaatttcact ctcacggttg ttgctgcaaa ttgcagcaaa gggtaatcga
     5041 tggttattga tcagaggatg tgacattcat cgcaacaatg gttgaccaat ttacataaca
     5101 tgtcgcccaa aataacaacg gttcaaccag gtaaggtaaa acgttatgca tgtgcttaac
     5161 attctctggg tggtattcgg cattggcctg atgctggtac tgaatttgaa gttcaaaatc
     5221 aattcaatgg tggctttgct agtggcggcg ctgtccgtcg ggatgctggc gggcatggat
     5281 ttgatgtcgc tgctgcacac aatgaaagcg ggcttcggca acacgctggg ggaactggct
     5341 atcatcgtgg tgttcggtgc ggtcatcggt aaattgatgg tcgactccgg cgcggcgcac
     5401 cagatagcgc atacgctgct ggcgcgtctc ggtctgcgct atgtacagct gtcggtaatt
     5461 atcatcggcc tgattttcgg tctggcgatg ttctatgaag tggcctttat catgttagcg
     5521 ccgctggtta ttgtcattgc cgccgaagct aaaatcccgt ttctgaaact ggcgatcccg
     5581 gcagtagcag ctgccactac cgcacattca ctgttcccac cgcagccggg tccggtggcg
     5641 ctggtgaatg cttatggcgc ggatatgggg atggtttata tctatggcgt actggtgacg
     5701 atcccaagtg tcatctgcgc aggtctgatc ctgccgaagt tcctcggcaa tcttgagcgc
     5761 ccaacgccat cattcctgaa agcagatcaa ccggtagata tgaataatct gccctctttc
     5821 ggcgtttcga ttctggtgcc gctgatcccg gcgatcatta tgatctctac caccatcgcc
     5881 aatatctggc tggtaaaaga tacccctgcc tgggaagtgg ttaactttat cggttcctcg
     5941 ccgattgcaa tgtttattgc gatggtggtt gcattcgtac tcttcggcac cgcgcgcggg
     6001 cacgatatgc agtgggtgat gaacgctttt gaaagcgcgg tgaagagcat tgcaatggtg
     6061 attctgatca tcggtgcggg tggcgtgctg aagcagacca tcatcgacac cggcattggt
     6121 gacaccatcg gcatgttgat gtcccacggc aatatctcgc cctacatcat ggcatggctg
     6181 atcaccgtac tgattcgtct ggcgacgggt cagggtgtgg tttcagcgat gaccgccgcc
     6241 gggattatca gtgctgcaat ccttgataca gcaactggtc agctggttgg cgtgaatccg
     6301 gcgctgctgg tgctagcgac ggctgcgggt tccaacaccc tcacccacat taatgatgcc
     6361 tcattctggc tgttcaaagg ttactttgac ctgtcagtaa aagacacgtt gaaaacctgg
     6421 ggtcagctgg agctggtcaa ctccgtggtt gggctgatta ttgtgttgat tatcagcatg
     6481 gtagcgtaaa agagcaccag agcctgccat tggcaggctc tggtcggatt gtcggcaaag
     6541 tgcgctttgt ttattcttat ctggcctaca aagggctaac gtgcgggttt gtagatagcg
     6601 tagcgcatca ggcaatattg cgtacctgca ttagcaatgt cctgtgattt ctttattgat
     6661 aaacaaaagt cacgccaata atcgattgca cattccctgc agtcacctgc cctccagtac
     6721 gtgcgtaatt tgccgttaat cccagactta ccgccgaagt ccctactgct cctaacgata
     6781 ccgtgttatt cgctggaata atcgtaccgt tgcgcgtcaa ctgtacgccg acgccctgcg
     6841 ctggtgaaaa cgaggcggta ttggtgaaaa tcgaattgcc cgcatctgcg gttgtgccgg
     6901 agaggtaata tcccaggttt tggcttttcg cacaataaac ggtaagagga attggcactg
     6961 aaccagggta gtccggcaga gtaacggtga catcacgagc agaaacatcg cagccgccag
     7021 tgggcaccac cacatcatta ttggcgtaaa tattccacac aaactgaaaa tcatcgctgt
     7081 tatagttgtt ggtctgtcgc aaaataagca cggcaattaa tgaaccagct ttaatcgcca
     7141 ccccgccagc actgctcaca ggcgtcaaat aaagcgccac cggccacggc ttatccgttc
     7201 tcgaattata aacaacccgc ggcgtttcgc tggtggtagg aaatggatag ctactgccac
     7261 tatattttac ggtcccggaa aaattagata acacgccgcc ataagccgag cctcgttgca
     7321 gtgtgacata gtctgtaatg gtttccggat aatcgttatg gcaaaagatt tgcgtcgaaa
     7381 gatccacgac caggttttgc cccacattca cggcaggcgc aaggtttaca taaacattag
     7441 cgctgccacc gccaataggg atagcggtgc cattggcggt tttacaggcg aatgaccagg
     7501 catttaccga ccagcccatc agcagtacag caaacagggt aataactcgt ttcattacaa
     7561 tcatctcttc gggttcagct gtaggtatag gtgatgctaa tcactgcctg aatggttccc
     7621 tgagtggctc cgccatttac cgtcaatgct ctgacctgta acgggaagtg cgctgattgt
     7681 gaggaatcat ccacctgaac tgttttggtt gcgccactat tcaatgtgtt gccactgtca
     7741 tcctgtagct ctaactggat gttttgcgcg gtcccctggt ttttataata tccggtactg
     7801 tcggctgccc cgctgaagct ggcagtgacc ctcgacgttc ccaccggaca attagtcaac
     7861 tcaagcgcaa catcatgcca ggccgatgcc gccccggcag acataagact gaaagaataa
     7921 agatcgccga gatctaccgt ggcattggtg gtggaaaccg tgcacggttt ggcgacgacc
     7981 ttaccgttca ccgtgatggt gacatcggct gcctgtatcg tcgcacttgc gagcgccaac
     8041 attgccgcca ataaataccc acgtttgcgc catttcatga gcatctccag ttactgatat
     8101 tcaagagtga aggtagccgt ggcattgata tgccccgcag tgacaggcac ctgtgtcgcc
     8161 attagccggg cgtaaaaatt cagcgtattt ggtttacccg gcgtcagggt cgtccacgaa
     8221 agcgcggacg atggggcatt aaggggtatc tgattttgtt gctcattcag aagctgtatt
     8281 cccagtcccg cagccgctga caccgtattt tcaagtgcaa gcaggttggc attgtggcta
     8341 tctgcaacac cggtaaaccc aacttttacg gcagaaacgg cattaccaca gggtgacagc
     8401 aaaatacgaa atggaacgac aggagtcgtc gcgccaatgt tgttaaattg cttcgccgcg
     8461 ttttccatca gatcaacagt aaaattggtt gattcagcgg ccacactaca gccgttatct
     8521 ctgacatagc cgcggatagt aatcgtgcta tccgcagcca aagcgcgact caccgccaac
     8581 cacaaaaaag cgcacagaag ataaaaaggt ttgtttctca tcacgccccc ttaacgacat
     8641 tcagctgata gctgggttaa taactgctgc tgactctctg gtggcagttg ataattggcg
     8701 acacagtgag cattttcctc ttctccccat ttcacctgaa cttttcccgc taaaggcatt
     8761 ccgctgaggt aaacctgacc attatccgca acaatgccgc tactctggct actctctgat
     8821 gtcaccatcg ccccaaacgg cagcggctta ttattgtggg ttagcgtcat gagcagtttt
     8881 atcccaacgc gcgctttaaa ctctgctcgc acgatcgccc cacgagtggg aacaacgtta
     8941 gcgaccgcgt tatctaaatc gacgttatca gccagggtat tggtatccag cgctactctg
     9001 ttttcccgat attcagtggc ataaggcagc acggcataac cgcgccagtc ggtacgcacc
     9061 cccgtctggt tttcgacttt tgcatctttt gcgccaggcg ctttaacaag caccaccgta
     9121 tcgtttaacg gctgccccag cgttacgcca ttggcatgag ccagtacccc accgctgact
     9181 ccgtaataga gctgcttaat atcatcgcta tggctgtaac cgatgttggc attgccgtaa
     9241 ccaccgcgat aattcagcgt ggcgtagcct gtacttccgc tattaccatc acctcccccg
     9301 gcgtaaccgg tttgcacgct atagctgagg ttgttgtctt ccagcaacgt accgtataca
     9361 ccggccagat tggtcatccg accgttgaga tcgtgtgaca tgctgtaact ggcactggca
     9421 tgtcgccact gagatttact gtcagaacgc agccagtggc tgaaaggaat attgacgtta
     9481 aggcagtatg actttc
//
LOCUS       DAZPDU010000164         9447 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZPDU010000164 DAZPDU010000000
VERSION     DAZPDU010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9447
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.166"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(81..308)
                     /gene="traY"
                     /locus_tag="ACHURS_003578"
     CDS             complement(81..308)
                     /gene="traY"
                     /locus_tag="ACHURS_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HFR1239215.1"
                     /translation="MIKGALRSAPSRKVLLTLDEETNKLLLGARERSGRTKTNEALVR
                     LQDHLQRYPDFYNEEIFREVIEENESTFKEL"
     gene            complement(428..1075)
                     /locus_tag="ACHURS_003579"
     CDS             complement(428..1075)
                     /locus_tag="ACHURS_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TraJ family protein"
                     /protein_id="HFR1239216.1"
                     /translation="MCAMDTRERALIRQSGLPEIILSAKYPVCVRTESGKFIDSNSVF
                     LHLIKCQNSDSEIWFSGIDIDTQILFHTAEVDAFSSSNGVSIINGVILNDVSWDVVVE
                     STEVNNLNLFVWRFFERYVVSGFFIKGKEFSCEPDKYRFNKIIPYLLGYSHEYSSQRM
                     DISVDQSKKIFMNFKKHYGFSDRDEWLRYAIISDVLFYLYQYFASNINSLHCKEM"
     gene            complement(1267..1650)
                     /gene="traM"
                     /locus_tag="ACHURS_003580"
     CDS             complement(1267..1650)
                     /gene="traM"
                     /locus_tag="ACHURS_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-binding protein
                     TraM"
                     /protein_id="HFR1239217.1"
                     /translation="MAKVNLYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLE
                     LGLRVYEAQMERKESAFNQAEFNKLLLECVVKTQSSVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIREKVSAEMERFFPKNDDE"
     gene            1971..2573
                     /locus_tag="ACHURS_003581"
     CDS             1971..2573
                     /locus_tag="ACHURS_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFR1239218.1"
                     /translation="MVLLVVRTTNLLSLFVEWVKLFTDKVTRGGKMKKWMLAICLMFI
                     NGICEAADCFDLAGRDYKIDPDLLRAISWKESRYRVNAIGINPVTGYGSGLMQVDSQH
                     FNELARYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVSWEAVGAYNAGFRKTERQNQ
                     RRLAYASEVYRIYTWIKSSKGIRVPTTKKSLSQINSVQKN"
     gene            complement(2870..3691)
                     /locus_tag="ACHURS_003582"
     CDS             complement(2870..3691)
                     /locus_tag="ACHURS_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFR1239219.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMHFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGKEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTENMETMKEIHLNSDEQHLFGRAALMARYEDENKTPVTPEQIITPRRWEDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWMIAEQFREWKS"
     gene            complement(3802..4098)
                     /locus_tag="ACHURS_003583"
     CDS             complement(3802..4098)
                     /locus_tag="ACHURS_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239220.1"
                     /translation="MSAFSFNTTYQPTGDQQKDAIAQIGIMQNRAVQANLAYQSCRDS
                     GALFKVLHQVNNELHDLLDSLENHTPLVRKNADELIALLLLFTRQVGQSRTDLI"
     gene            4398..4694
                     /locus_tag="ACHURS_003584"
                     /pseudo
     CDS             4398..4694
                     /locus_tag="ACHURS_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4995..5120)
                     /locus_tag="ACHURS_003585"
     CDS             complement(4995..5120)
                     /locus_tag="ACHURS_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HFR1239221.1"
                     /translation="MLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK"
     gene            complement(5062..5211)
                     /gene="mok"
                     /locus_tag="ACHURS_003586"
                     /pseudo
     CDS             complement(5062..5211)
                     /gene="mok"
                     /locus_tag="ACHURS_003586"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid maintenance protein Mok"
     gene            complement(5433..6152)
                     /locus_tag="ACHURS_003587"
     CDS             complement(5433..6152)
                     /locus_tag="ACHURS_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFR1239222.1"
                     /translation="MSARSQALVPLSAEQQAAWRAVAETEKRRHQGNTLAEYPYAGAF
                     FRCLNGSRRISLSDLRFFMPSLTAEELRGNRSQWLYAVDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFRVRERSRHKSALVMQKYSRQQAREAEQKARAYQALVAQAEIELAFHSPE
                     TVGSWHARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVIAEASLAARE
                     AGHAVREMERWMVPNKLREAA"
     gene            complement(6149..6583)
                     /gene="psiB"
                     /locus_tag="ACHURS_003588"
     CDS             complement(6149..6583)
                     /gene="psiB"
                     /locus_tag="ACHURS_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFR1239223.1"
                     /translation="MKTELTLNVLQTMSAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDAQGYSVSDIIHILMAEGGQA"
     gene            complement(6652..8616)
                     /locus_tag="ACHURS_003589"
     CDS             complement(6652..8616)
                     /locus_tag="ACHURS_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFR1239224.1"
                     /translation="MSVTKSEPKSTRKASRKPAKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELADSIKGVGLLQNLVVHALPGDRHGVAAGGRRLAALNMLA
                     ERGIIPADWPVRVKIIPQELATAASMTENGHRRDMHPAEQIAGFCAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDLITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEHLREAEGWGWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAEE
                     ERLNELMTRYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDAADDADRTEQVQEKAPVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFGSGAYSKPAQISLECKHYSLTSDAPSGKDGAAFLALMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSIHGVQTREHGHTSRSPLDSLESAI
                     GFHMRDWWQPTKANFFGHLQKPQIIDALNDAGLSGAARDAEKMKKGDAAEHAEFHMKD
                     NRWVPGWMCTPRPQAETETTEYRDDQAEAA"
     gene            complement(8677..8910)
                     /locus_tag="ACHURS_003590"
     CDS             complement(8677..8910)
                     /locus_tag="ACHURS_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFR1239225.1"
                     /translation="MSEYFRILQGLPDGPFTRKQAEAVAAQYRNVFIEDDHGEQFRLV
                     VRNNGTMVWRTWNFEDGAGYWMNHVIRDFGILK"
     gene            complement(8968..>9447)
                     /locus_tag="ACHURS_003591"
     CDS             complement(8968..>9447)
                     /locus_tag="ACHURS_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFR1239226.1"
                     /translation="KDPEVRYIPNGGAVANLQVATSETWRDKQTGEMKEQTEWHRVVL
                     FGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEILVKTTGTMQMLGSAPQQ
                     NAQVQPQPQQNGQSQSADATKKGSAKTKGRGRKAAQPEPQPQPPEGEDYGFSDDIPF"
BASE COUNT         2324 a         2576 c         2187 g         2360 t
ORIGIN      
        1 ttttcaacgg gcgcagaagc accctgaaca cttaaaacag tattcatatg attcctctga
       61 ttaatattga cagatgaaaa ctaaagttct ttaaatgttg actcgttctc ttcgatcacc
      121 tccctgaata tttcttcatt ataaaaatct ggataacgtt gcaaatgatc ctgcaacctc
      181 accaatgctt cgtttgtttt cgttcttcca ctacgctctc tggctccaag taataattta
      241 ttcgtttcct catcaagggt aagtaaaact tttctacttg gcgcagatct taaagctcct
      301 ttaatcaccc tccctcccat aaataaaaga gatttagtca aaatattcac ataagacgca
      361 cacaaaaata aacaacaaaa tagtaaatta cgttaacaaa aaccggcact tttagtgccg
      421 ggaatgctta catttcttta cagtgaaggc tgttaatatt actagcgaag tattgatata
      481 gataaaataa cacatcagag attatagcat atcgcaacca ctcatccctg tctgagaaac
      541 catagtgttt tttaaaattc ataaatattt ttttagattg gtcaacagag atatccattc
      601 tctgagaaga atactcatga ctataaccca aaagatacgg tattatctta ttaaagcgat
      661 atttgtcagg ttcacaacta aactccttgc cctttataaa aaagccacta actacgtatc
      721 tttcaaagaa tctccagaca aacaaattca agttattcac ttccgtactt tcaaccacca
      781 catcccatga tacatcattc aatattacac cattaataat actcactcca ttcgaggacg
      841 agaaagcatc aacttcagca gtatgaaata aaatttgcgt atcaatatca ataccagaaa
      901 accatatttc agaatcacta ttctgacact ttattaagtg taaaaaaaca gagtttgagt
      961 ctatgaattt tcctgactct gtgcggacac agactggata ttttgcggat aaaataattt
     1021 ccggcaggcc tgattgacgt atgagtgctc tttctctagt atccatcgca cacataggaa
     1081 cctcctcaaa ggattctatg gacagtcgat gcagggaggg acaagctccc tgcatcggcg
     1141 attttcaagt tcaaatttta acgtggcatt aaccacgtaa ttaatatact aattattctg
     1201 attattgtca tcagtttgaa ccattaaaaa gtctgatatt tgaacaaagt caaaatttat
     1261 ttccctttat tcatcatcat tttttggaaa gaacctttcc atctcagccg acaccttctc
     1321 tctgatatct tcaaccatat tggcatattc aaactttgga tttcctgaga catgaggact
     1381 gagagactca atacccaaaa ttttcgctac tgatgactgt gtttttacaa cgcattcaag
     1441 aagtagttta ttaaactcgg cctgattaaa tgcagactct ttgcgctcca tctgagcctc
     1501 atatacacga agacccagtt caagaagcat tgaagccgtt gctgaaaaac tgacatcttt
     1561 ttctctcgcc ccctcctgtc gacgtttttc aataatcgcg tttatttttt cataagcatc
     1621 attgctgata tacagattca ccttagccat tagatattac cttttttaaa cataaaacaa
     1681 agttaatttc gaaacactag aattcatagt gcaaacaggg acgcaccgct agcagcgccc
     1741 ctgacggtat cctataaaaa aacacaccgc gccgctagca gcacccctaa tataaaataa
     1801 tgttttttat aaaaatagtc agtaccaccc ctacaaagcg gtgtcggcgc gttgctgtag
     1861 ctgcgttaac gacgctgctt taaaaaaatc agatttaaac aatataaatc cacaaataca
     1921 actcaatgat attaaagata aatcagcaaa aacttgtttt tgcgtagtgt gtggtgcttt
     1981 tggtggtgag aaccaccaac ctgttgagcc tttttgtgga gtgggttaaa ttatttacgg
     2041 ataaagtcac cagaggtgga aaaatgaaaa aatggatgtt agcaatctgc ctgatgttta
     2101 taaatgggat ctgcgaagcc gccgattgct ttgatcttgc aggtcgggat tacaaaatag
     2161 acccggattt actgagagcg atatcatgga aagaatcccg ttaccgggtt aatgccatcg
     2221 gtattaatcc ggtaacggga tatggcagcg gactgatgca ggtagattcc cagcatttta
     2281 acgaactggc ccgctatgga attaagccgg aacatctgac aacagatccc tgtatgaaca
     2341 tttataccgg tgcttattat ctggcaatag cctttaaaaa atggggcgtc tcctgggagg
     2401 ccgttggtgc atacaatgcc ggattcagga agaccgaacg ccagaaccag agacgtcttg
     2461 cctacgcatc agaggtttac cggatttata cctggataaa gagcagtaaa ggcatccggg
     2521 ttccgaccac gaagaaatca ctttcccaaa ttaacagtgt gcagaaaaat taatattgta
     2581 tacattgtca aataccggtt atgccgcgac agcggcatgg aaggccacac acaaaaatat
     2641 catatgagtg ataatgctga ctggccgaag ccccgcaaag cgggatccca tcttatatat
     2701 atccggtaac tgcgcagcct cctgccagac catttttcgc gggcacaata tttacagact
     2761 accgaaaagc cagttgatga aattgaggca tatcagacga cacagcaatg ccgcaggatg
     2821 ataatataag taaagccccg aaaaattttt cggggcttta cttatattat catgacttcc
     2881 actccctgaa ctgttcggca atcatccata atgctttgtt gattcggata tcaccgtcaa
     2941 tgcctgtaat ggctcttgtt ctggtatttt taccggaggc acttcgcccc gataatccac
     3001 cttttatcat attctcctga acccgctgcc aggttgtcca gagatcgttc tgtttatcct
     3061 cccaacggcg gggagtaatt atttgttctg gcgtcactgg cgttttattt tcatcttcgt
     3121 acctggccat caatgcagct ctgccaaata aatgttgttc gtcactgtta agatgtatct
     3181 ctttcattgt ctccatattt tcagtgactt tatcaaagac accgagcact tcatacgctc
     3241 cctcaataac ctgcccgaca atatcacctt tatgtggaac gcggatttcg ccaaaattat
     3301 tcccacacac caggccattt gtgcagacaa aacgaaaaat tcctgggatc atctgataac
     3361 tggatgaacc atcatgtgaa ttaagcagga taatttcagg aacttctttc ccgttaatat
     3421 gcccttccct gcgaagacgt aacatatgtt tactgtattc gcggcgtccc aaatcacgaa
     3481 cccgactctg acaggcaaag aatggctgga aaccttcatc acgtaactta ttgatgatat
     3541 tgatggttgg gatatatgta taacgctcac tccgggattc atgtttatca ccagaaaaga
     3601 cagatggaac aaaatgcatt aattcgtcat ccgttaacgg gcgctcacgg cgaatagaat
     3661 tatatcgacc aaaacgggaa gctaatcgca taacagtaac tcctttatga aggcataaaa
     3721 ttaataaaag ttttttcgct taatatcatc ccccctcggt tatcgttaac aaaaagacaa
     3781 caattccgag gggaaggacc atcaaatcag gtcagttcgg gactgaccaa cctgccgagt
     3841 gaaaagcagt aacagcgcga tcaactcatc agcgtttttt ctgacaagag gagtatgatt
     3901 ctccaggcta tccagtaagt catgcagctc attattgacc tggtggagca ccttaaagag
     3961 tgcaccagaa tcccggcagc tctgatatgc aagatttgcc tgaacagcac gattctgcat
     4021 gatcccgatt tgtgcgattg cgtctttctg ctgatcgcct gttggctgat aagtggtgtt
     4081 aaaagaaaat gcgctcataa ttcctcctgt atctgatgtt ttcagccccc atcatctgtt
     4141 gagtttcggg agccgcggtt ttgacgtttt gcggggacct gccttttgtt ccctccggta
     4201 ttcaccatca cctgtttgag aaccttcgcc gcaaggggca tcactgccac ggcctgacgc
     4261 acctgtcctg ggcagaacgc aaaatttttt accgggtttt gggtaaaaaa ttttgtgggc
     4321 tgttcattta ccctggacag tcagtgcggc aggttgtggc tgtctatgcc cccggcgaca
     4381 ggggcttaaa caggtgaatg gcaccggaca gaacaacggg cagggacccg ccgcaacgac
     4441 gtgaagcgcg gttcgcgaaa cggcgttgct ccttacatcc cggcgtcttt tgccgggatg
     4501 ccgggagcgc aggccacggc gcgacaaggg tggcgcgacc gttccggtcg ctgcaaaccc
     4561 gggtttttgc gtcagggatg gaagcccgcc agggcggaga cagcttcgct ggctccgttc
     4621 acgacagccc gccccgccag ggggacgccc ggaaaaggca taagcaggag aacaacaaaa
     4681 cagacaataa tcagggcagc agtaatcaga gtcagagcag gaggaaaaag agaataatca
     4741 aagggaaggt gatgtgattt ctggtaagtc ataaacagag aaggcggggg aaaagaacgg
     4801 gcgccgcagg cgcacgttca tccccgttcc cggagggaaa cggccttcct ggcagtctgg
     4861 ttgttcatgc ccgtacaatg ccagcaagga gaaaggggcc accggcgaac cagcagcccc
     4921 tttataaagg cgcttcagtg cagtcagacc agcatcagcc ctgaaaaggc gggcctgcgc
     4981 ccgcctccag gttgctactt accggattcg taagccatga aagccgccac ctccctgtgt
     5041 ccgtctctgt aacgaatctc gcacagcgat tttcgtgtca gataagtgaa tatcaacagt
     5101 gtgagacaca cgatcaacac acaccagaca agggaacttc gtggtagttt catggccttc
     5161 ttctccttgc gcaaagcgcg gtaagaggct atcctgatgt ggactagaca tagggatgcc
     5221 tcgtggtggt taatgaaaat taacttacta cggggctatc ttctttctgc cacacaacac
     5281 ggtaacaaac caccttcacg tcatgaggca gaaagcctca agcgccgggc acatcatagc
     5341 ccatatacct gcacgctgac cacactcact tttcctgaaa ataacccgct cattcaggct
     5401 gtttacgggt aatctgtgtg attgttgccg catcacgccg cctcccggag tttgttcggc
     5461 accatccacc gctccatctc acggacagcg tgaccggcct ctcttgctgc aagactggct
     5521 tccgcaataa cctgccagaa cggcatatcc tgccactgcc agcgttccat tccggccagt
     5581 gacggaaaac gctcacccca ctgccagaac agggtttcca ggtcatgctc tgccacccgg
     5641 tcactccagc gggcatgcca gcttccgact gtttccggcg aatggaatgc cagttcgatt
     5701 tcagcctgag cgaccagcgc ctggtacgca cgggctttct gctctgcctc cctcgcctgc
     5761 tggcggctgt acttttgcat gaccagtgcg gatttgtggc gactgcgctc cctgacacgg
     5821 aaccgcaccg acgggaacag ccgctctgct gcatcgccgg gaaggggaag aaggcagacc
     5881 tcgccctgag tttcaatcag cacatcgacg gcatacagcc actgagaacg gttgccgcgc
     5941 agttcttctg cggtcaggga cggcatgaaa aatcgcaggt cagacagcga tatcctgcgg
     6001 cttccgttca gacagcggaa aaacgccccc gcatacggat attccgccag cgtgtttccc
     6061 tggtggcggc gtttttctgt ctccgccacc gcccgccatg cggcctgttg ctctgcgctg
     6121 aggggaacca gtgcctgtga acgtgcgctc atgcctgacc tccttccgcc atcaggatat
     6181 ggatgatgtc actgacgctg tacccctgcg cgtccagtga cgccgccagt gccagtgaat
     6241 ggctgacggc ttccggtgca aaccgccgct gtacctgaac caccgcaaac ggctcaccac
     6301 cggcattcac cagaaccagc acccagacct gagagacatc gcccggtgaa cataacgcca
     6361 ggtgaaaacg ttcgtgggca ggcgtgaaac ggacctggac ggggaaaaag ccgccgaact
     6421 cgctgccata ctcgccgttc atcgtccagt tatccggcgc atccagctcc cgcatcacgg
     6481 catgtgtgag ttcacgacgt tcatcacttc cggcagcccg gatatcttca tattcctgtg
     6541 cgctcatggt ctgtaataca ttcagggtca gttcagtttt catcacggta tctcccttgc
     6601 tgccgccccg tctccggcag ggcggcgtga taaatggttt atgactggcg gtcaggcggc
     6661 ttctgcctgg tcgtcacggt actccgtggt ttccgtctct gcctgtggac gtggtgtaca
     6721 catccagcca ggcacccagc ggttgtcttt catgtggaac tctgcatgtt cagcggcatc
     6781 gcctttcttc attttctccg cgtcccgcgc agcaccggac agtccggcat cattcagggc
     6841 atcgataatc tgcggctttt gcaggtgtcc gaagaagttt gctttcgtcg gctgccacca
     6901 gtcgcgcatg tggaatccga tagcgctctc cagcgagtcc agcggactgc gtgacgtgtg
     6961 accatgctcg cgggtctgaa cgccgtggat gctgcacgcg gtgcagaaac tgagcaggga
     7021 taacagtacc tcctggctga gtgacaggaa cgtcgtcata tcccgtgacc atccctccgg
     7081 taacagggct gcaagacggg ctttttctgc catcagcgcc aggaatgcgg caccgtcctt
     7141 ccccgatggc gcatcgctgg tcagcgaata atgtttacat tccaggctga tttgtgctgg
     7201 tttactgtac gctccgctgc caaacacatt caggcagagc gtccatgcca gcagtgccag
     7261 agatttgtcc ggttgctgca tcagtgctgc ctggactgcc agcgtgcgtt cggaagacat
     7321 tttcgtcagc agtggcagac tgatttcctc cacgggcgct ttctcctgca cctgttccgt
     7381 gcggtcagcg tcgtcagccg catcatcctc gctgcgcaac tgcacaccac gctggacaca
     7441 cacgttgcca taacgccagg acaccaccac accgcttccg gcacgcatct ccggcgtcca
     7501 cgccctgacc ttcgccatgc agcgcatcag cttcatttct gcttccagca ggtcagattc
     7561 ctcacactgg ttttccagcg cgtcgtaacg cgtcatcagt tcgttcagac gttcttcttc
     7621 cgcctccgtc agcaccgctt ccggctccgg cagacagcgg tatgttccgg catcctcacg
     7681 gcactcaccg acaggctcca tgcgtccggc gcaccatccc cagccttcgg cttcccgaag
     7741 gtgttcagca acagcctgga gtttttccag cagggcggca tcgagcgcca cgcagtccac
     7801 atagccaccc tcgtcgtcgc tgaacaggtc agtgcgcagt tcatctggcg agaaggcatc
     7861 agcccccacg aagcggaatt tactgttccc tgccaccgcc acttcacttt cggtcaccag
     7921 acgacgaatg gtctgtactt ccggtttacc gccccatccc gactgacagg cggcttcaaa
     7981 cacctgcacc tgacgcgcgg tgtcgttctc cagcgccagc gcctgacagt gctcggtggt
     8041 gatgaggtct tctgccagcg catcgaggat gacaggcgca aggtcagcca gtttcagcat
     8101 tcgctgaacg tggcggggtg aatagcccag caaatcaccg atttgtgcag gtgttttgcc
     8161 ttcctgcgcc attgcacaga atccggcaat ctgttcggct gggtgcatat cccgacgatg
     8221 accgttctcg gtcatcgatg cggcagtcgc cagttcctgc ggaataattt tcacgcggac
     8281 aggccagtca gccggaatga ttccacgctc tgccagcatg ttgagtgctg ccagtctgcg
     8341 accacctgcg gcgacaccgt ggcggtcacc tggcagggca tgaacaacca gattctgcag
     8401 caggccgacc cccttaatgg aatcagccag ttcgctgacg gactccgcag aatacggcac
     8461 cgtgcgcaca ttcagtggtg acttaatcag cgaggccagc ggcacgctca cttcctccgt
     8521 ctgcgccagc agggcagaca ggacagtttc ctgcgttttt gcaggtttac ggcttgcttt
     8581 acgggtagat tttggttcag actttgttac tgacatgact atccccttat tgtgtagtta
     8641 atgtctgccg gacgtgatcc ccgtccggca cctctcttac ttaaggatcc cgaaatcacg
     8701 gatgacatgg ttcatccagt acccggcacc gtcctcaaaa ttccaggtac gccagaccat
     8761 tgttccgtta ttacggacaa ccaggcgaaa ctgttcgccg tggtcatcct cgataaagac
     8821 gttccggtac tgtgcggcaa cggcttcggc ctgtttgcgg gtaaaaggac cgtcaggcag
     8881 tccctgaagt attctgaaat attcgctcat cctcatatct ccggtgatgc cccgcgcagg
     8941 acggggcggt tatcacagtc aggccagtca gaacgggata tcgtctgaaa acccgtaatc
     9001 ctccccctcc ggtggttgcg gctgaggctc cggctgcgca gccttacgtc cacggccttt
     9061 cgttttcgcg ctcccttttt tcgtcgcgtc agcactctgt gactgaccat tctgctgagg
     9121 ctgcggttgc acctgagcgt tctgctgtgg tgcactcccc agcatctgca tggtgcccgt
     9181 ggttttaaca aggatttcgg tgacgtaacg ggtaataccg ttatcttccc agctacgggt
     9241 gcgaagctgg ccttcaatgt agacctgcgc ccccttacgc agatattcac ctgccacttc
     9301 cgcgagcttg ccgaacagca ccacgcggtg ccattccgtc tgctccttca tttcccccgt
     9361 ctgtttgtcg cgccaggttt ctgacgtggc cacctgcagg tttgccactg cgcccccgtt
     9421 ggggatgtaa cggacttccg gatcttt
//
LOCUS       DAZPDU010000165         9427 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZPDU010000165 DAZPDU010000000
VERSION     DAZPDU010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9427
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.167"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..173
                     /locus_tag="ACHURS_003592"
     CDS             <1..173
                     /locus_tag="ACHURS_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFR1239227.1"
                     /translation="RNRRLDFCADAIRHAADDEKLAGIGFHWGFSDQSHFSTVFKQRF
                     GMTPGEYRRKFR"
     gene            complement(345..674)
                     /locus_tag="ACHURS_003593"
     CDS             complement(345..674)
                     /locus_tag="ACHURS_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFR1239228.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(679..864)
                     /locus_tag="ACHURS_003594"
     CDS             complement(679..864)
                     /locus_tag="ACHURS_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFR1239229.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(861..3500)
                     /locus_tag="ACHURS_003595"
     CDS             complement(861..3500)
                     /locus_tag="ACHURS_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFR1239230.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGMVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVIAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHEYELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPINATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3708..4697
                     /gene="ldhA"
                     /locus_tag="ACHURS_003596"
     CDS             3708..4697
                     /gene="ldhA"
                     /locus_tag="ACHURS_003596"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFR1239231.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4808..5230
                     /gene="hslJ"
                     /locus_tag="ACHURS_003597"
     CDS             4808..5230
                     /gene="hslJ"
                     /locus_tag="ACHURS_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFR1239232.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(5227..5493)
                     /locus_tag="ACHURS_003598"
     CDS             complement(5227..5493)
                     /locus_tag="ACHURS_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFR1239233.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVALGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5767..9291
                     /gene="nifJ"
                     /locus_tag="ACHURS_003599"
     CDS             5767..9291
                     /gene="nifJ"
                     /locus_tag="ACHURS_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFR1239234.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPCVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQTLPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGKRETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTQPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARKCGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         2346 a         2461 c         2304 g         2316 t
ORIGIN      
        1 ttcgtaaccg tcgtctcgat ttttgtgcag atgcgattcg ccatgccgca gatgatgaaa
       61 aactggcagg catcggcttt cattggggat tttctgacca gagtcatttt tcaacggtat
      121 ttaagcaacg ctttgggatg acgccaggcg agtatcgacg taaattccgt taactaattc
      181 gcgttgtgtg tactgcaacg ccaattagtt aaaacagata tttgactatg catttttcag
      241 ggcaaaaggc aatttgacag gagtttttga atattcacct tgtttcagga aaatagatca
      301 atcgctttct cctgtagcgt tatcccgata ttgcggtttt gctattaaaa ttttcgcacc
      361 catttaccgt ttatcccttg tacatattcc cccggtttgg cccgagctac gagcttttgc
      421 ccggcgagtt tcgcgatatc atctaccgac acattatttt gctttgccag ttgttggtaa
      481 ctcgctttac gttcggcatt aatgtcggtt actaatttct ctgtttcagc atccgttttc
      541 agcgcaacca gataaccata aaatgtttca cctacccgcc cctgagttct ggcttcatcc
      601 agagtcaatg ccattacatt gctgcttacc agcccaacaa gaaacgcaca aagaagtaat
      661 gttttcttca tcatcgcctc agaaaagatc gctacgagtt tcaagcagct cttcgacatc
      721 tttgtctgcc ttgatgatga tctcatgctc aattttaacg ttcatattga tagtgatcgg
      781 ttccttgggc gcagcgactt caatacgcgg cgtacagcca accagcataa aagatgacgt
      841 caacgcagcc agtaaaattt tcattgtttt tcctcacact cttttccttg cggacagtca
      901 tttcccggca gacgtgcgtt ctgctccaac catgcctgga gattatcgcc aaagcgtaaa
      961 ctgcgccaca gcgtaaaaat attctcttca tgatggtaat taaggtttac cgtaccgctt
     1021 ttaccatcaa cgcgactggt acctgtaatg ttggcctgca tggttaataa accgagatta
     1081 tctaagttaa tttttgtcga tgaccggcta atttccatat agcgcaacca gttaattgct
     1141 gaacccgcag tcatattgtc tttcaccacc gcatctgcgg tgtctttatc cagccgcaag
     1201 gtcattggcc cgctatttgc cagccaacca tctttcacta tccatttttc gttgtttagc
     1261 cataacggca atgcaccact aaatgccccg gacatggcga attgtttcgg attgacggcg
     1321 ctaaccagtt cgctggatga gagattattc agccgcaaca gcgccgggtc atgttgcggc
     1381 atacgtaatt gtttcatcag tacgttaccg cctaacacat cgacgctaac atcagtcaac
     1441 agcaagggtt cttcttcagt ccacggataa cgcccttgca aatcagccgt aatatttttc
     1501 gctgtcacca gattaatcac ttcggcaatt cgcaacgtaa cggggccgcg agtccccaga
     1561 tgccaggctc catcggcaaa acggaaaggc aggacaaaat cgacaccgtt aacctgatta
     1621 tctggcatcc aggcactacc gcctttcaac acgccgtgtc ctcccgcgcg gaatccttgt
     1681 tcaggcgcag cagaaaatgc aacctgagca tacagttccc catcgcgtaa gttcatcttc
     1741 cagtcgggtg gcaccagcgg ctggaataca gtcagtgatt gttttggcca ccaggcgtta
     1801 ccgcgcagac gaataccgtc ccagcgacca tttacccgaa ccgggccaat ctcaccagca
     1861 tgtaaatcgc ctttaaagag gaaatacgtc ggatcgcgcc catcgacgct aaattttaag
     1921 gttgatggcg gtaacacact gccgccagtg aacagcgttt gcccggcgtc cagtgacagc
     1981 gcgccgctga aggagggatg ctgtgcgtca cgtacccaga cgacgggctt gtcgagaatt
     2041 aatcgcggct tttctaccgt catcgtaccg tattgtaact ggtcgaaacc ggtggagagg
     2101 tcggtcagcg taatcgtgct gtcatgccat tcaccttttc ctgcgacatc ccaggtggca
     2161 tttatcggtg taaaactgcc ttttccccag tagcgccact gccagcggcc cgcgtcaggg
     2221 agaaaatcat tcgccagccc atccatatgc agcacgaaat cgcccagttc atattcatgc
     2281 gcctgcaaaa tggcctgcaa acgtccgtca acaccacgtt gggtgacttt tacacccgct
     2341 aaaggccagc ggatttcatc gatgtccagc gaatcgatga ctcttccctt cgaacgtaat
     2401 aacgcgcctg gctcaaaggc cagcgttggg tcagtcagac ttccacttag ctgcgcgggt
     2461 aagcgggcat ataaaatgag gtccgcctgt ttcgcttcac cggtcagctg cagaggcagc
     2521 tggctgttat ccatgcttaa ttttcctggg ccaaaattta gcaccgcgtt gcctttaccc
     2581 gcttgcccct gggtcagcac actcagtcgt ccgctgatca gagcgttctc aagccctgcc
     2641 tgccagttgt cgactttgac acccagtcgg ccactcaaag gaaaacctgc atacggccaa
     2701 ctccagcgac catcgcttac ggtcaattgt tgacgagtaa tttgccacgg caaatcgagc
     2761 aacggatcgc cgttatcccg tgccagcaca atcaattgcc cgctattttc ctgccagtcc
     2821 agctctgcat ccaccagtga cggttcctgc ggcaagttta acgtcgcagt agcatgccca
     2881 cttacaggaa gtccatccgg cacgagcggc atagtaaatt cccccaccag tttaaccggc
     2941 ggctgatttt caaacgcgat gacatccagt tcgctgactg taagttgttg ccctttcagc
     3001 tggccttgaa atttaacttt ttcgccctga taacgcagtt gctgaatatc agaggttaat
     3061 gcgagagaga gttttccctg ccattcctgc cagggagaaa aaatcagttt atcgatattg
     3121 atccaggtgt taggcagcat ggactgccac tgcgcgaggg tttttggagc agctggcgat
     3181 tgctccgtct gcggcaattt cgccagacaa gcagaatcaa gttctaccat gccgacgttc
     3241 aataaccagc ggctgggatg tgaaaggctg gcgttggtga tatgcgcaag ctgacaatct
     3301 cccaccagat aacggagatc gggaatgatt aaacctttac gcgtaatgcg tgggctttca
     3361 tctaatgcaa tacgtgtccc gagcggtagc cagatgcccg ccagcgtggg aacccacagc
     3421 ccgagcgtca tcagcagcgt caacggcaca agaataatca gtaataacag cgcgagaacg
     3481 gctttatatt tacccagcat gggtagttaa tatcctgatt tagcgaaaaa ttaagcattc
     3541 aatacgggta ttgtggcatg tttaaccgtt cagttgaagg ttgcgcctac actaagcatt
     3601 gttgttgatg aatttttcaa tatcgccata gctttcaatt atatttgaaa ttttgtaaaa
     3661 tatttttagt agcttaaatg tgattcaaca tcactggaga aagtcttatg aaactcgccg
     3721 tttatagcac aaaacagtac gacaagaagt acctgcaaca ggtgaacgag tcctttggct
     3781 ttgagctgga attttttgac tttctgctga cggaaaaaac cgctaaaact gccaatggct
     3841 gcgaagcggt atgtattttc gtaaacgatg acggcagccg cccggtgctg gaagagctga
     3901 aaaagcacgg cgttaaatat atcgccctgc gctgtgccgg tttcaataac gtcgaccttg
     3961 acgcggcaaa agaactgggg ctgaaagtag tccgtgttcc agcctatgat ccagaggccg
     4021 ttgctgaaca cgccatcggt atgatgatga cgctgaaccg ccgtattcac cgcgcgtatc
     4081 agcgtacccg tgacgctaac ttctctctgg aaggtctgac cggctttact atgtatggca
     4141 aaacggcagg cgttatcggt accggtaaaa tcggtgtggc gatgctgcgc attctgaaag
     4201 gttttggtat gcgtctgctg gcgttcgatc cgtatccaag tgcagcggcg ctggaactcg
     4261 gtgtggagta tgtcgatctg ccaaccctgt tctctgaatc agacgttatc tctctgcact
     4321 gcccgctgac accggaaaac taccatctgt tgaacgaagc cgccttcgat cagatgaaaa
     4381 atggcgtgat gatcgtcaat accagtcgcg gtgcattgat tgattctcag gcagcaattg
     4441 aagcgctgaa aaatcagaaa attgggtcgt tgggtatgga cgtctatgag aacgaacgcg
     4501 atctattctt tgaagataaa tccaacgacg tgatccagga tgacgtattc cgtcgcctgt
     4561 ctgcctgcca caacgtgctg tttaccgggc accaggcatt cctgacagca gaagctctga
     4621 ccagtatttc tcagactacg ctgcaaaact taagcaatct ggaaaaaggc gaaacctgcc
     4681 cgaacgaact ggtttaatct tgccgctccc ctgcattcca ggggagctta ttcagataat
     4741 ccccaaagac ctttcatctt ctattcttaa aatagccctg agtcagaaac tgtaattgag
     4801 aaccacaatg aagaaagtag ccgcgtttgt tgcgctaagc ctgctgatgg cgggatgtgt
     4861 aagtaatgac aaaattgctg ttacgccaga acagctacag catcatcgct ttgtgctgga
     4921 aagcgtaaac ggtaagcccg tgaccagcga taaaaatccg ccagaaatca gctttggtga
     4981 aaaaatgatg atttccggca gcatgtgtaa ccgctttagc ggtgaaggca aactgtctaa
     5041 tggtgaactg acagccaaag ggctggcaat gacccgtatg atgtgcgcta acccgcagct
     5101 taatgaactc gataacacca ttagcgaaat gctgaaagaa ggtgcacaag tggatctgac
     5161 cgcgaaccag ttaacgctgg cgaccgcgaa acagacatta acttataagc tggcggattt
     5221 aatgaattaa tagctgccac agctcccggc ggcaagtgac tgttcgctac agcgtttgcc
     5281 gttgggtaat gcacacatcc caatcgccgt accatccagt tgacgggcaa cagaaagcga
     5341 accgccgatc attgcacaat ttgcttctcc actactggac atcgacgctt ttaaacctag
     5401 cgctacgtgc gccgcagtcg cctgctgaac aggttcacta ctacacgccg acaacaataa
     5461 agcggcacac cctacccaaa acgctgctcg catctcttct tcctctgatc ttcaagccaa
     5521 acgacaccgc cataaataat aggcagcaca gagggcggcg tcgagagctg tcctgcgcgt
     5581 tgccccgcat ttttactttt ttatggctat ttttttgccc tctgtttgat caaaacattc
     5641 attacgctga tgtgggggac acaaaagcga aaatgcagaa gaaagccatt tgctaaaatt
     5701 gaaggattac tactgggcgc gcagcaattt cgtgcgcccc tcatttgcgc aatgtaaggg
     5761 tgtcatatga ttactattga tggtaatggc gcggttgctt cggtggcgtt tcgcaccagt
     5821 gaagttatcg ccatctaccc tattaccccc agttccacga tggcagaaca ggctgatgcc
     5881 tgggccggaa acggcttgaa gaacgtttgg ggagacacac catgcgtggt tgaaatgcag
     5941 tcggaagcgg gtgctatcgc taccgtgcat ggcgctttgc agacgggtgc cctttcaaca
     6001 tcgtttacgt catcgcaggg tttgctgctg atgatcccga cactgtacaa actggcaggc
     6061 gaactcacac cgtttgtcct gcatgtagcg gcacgtaccg ttgccacaca tgcactctct
     6121 atttttggcg atcattccga cgtcatggcg gtgcgccaga cgggttgcgc gatgttgtgt
     6181 gcggcaaacg tccaggaagc gcaagacttt gctctcattt cgcacatcgc gacgctgaaa
     6241 agccgcgtgc catttattca tttctttgat ggtttccgca cgtcccacga aatcaacaaa
     6301 attgtcccgc tggccgatga cacgattctt gatctcatgc cgcaggccga aattgatgct
     6361 catcgcgccc gggcgctcaa cccggaacat ccggtgatcc gtggtacgtc cgccaatcct
     6421 gacacttatt tccagtctcg cgaagccacc aacccctggt acaacgcagt ctatgaccat
     6481 gttgaacagg cgatgaatga tttctctgcc gcgacaggtc gtcagtatca gccgtttgaa
     6541 tattacgggc atccgcaagc tgaacgggtg attatcctga tgggctcagc cattggcacc
     6601 tgtgaagaag tggttgatga attgctaacc cgtggcgaaa aagtcggcgt gctgaaagtt
     6661 cgcctgtacc gccccttctc agccaaacac ttgctacaaa ctctaccggg atccgtacgc
     6721 agcgtggcgg tactggacag aaccaaagaa cccggtgccc aggcagaacc gctctatctg
     6781 gatgtgatga ccgcactggc agaagccttt aataatggca agcgcgaaac tctgccccgt
     6841 gtcattggtg ggcgctatgg tctttcatcc aaagaatttg gcccggactg tgtactggcg
     6901 gtatttgccg agctcaacgc ggctaaaccg aaagcgcgct ttacggttgg tatttacgat
     6961 gatgtaacca atctgtccct gccgttgccg gaaaacaccc agccaaactc ggcgaaactt
     7021 gaagccttgt tttatggcct tggtagtgat ggcagcgttt ccgcgaccaa aaacaatatc
     7081 aagattatcg gtaattccac gccgtggtac gcacagggct attttgttta cgactccaaa
     7141 aaagcgggcg gcctgacggt ttctcacctt cgcgtgagcg aacagccgat tcgttccgct
     7201 tatctcattt cccaggctga ttttgttggc tgccaccagt tgcagtttat cgataaatat
     7261 cagatggctg agcgtttaaa acctggcggc attttcctgc tcaacacgcc gtacagcgca
     7321 gatgaagtgt ggtcgcgctt gccgcaagaa gttcaggccg tgttaaacca gaaaaaagcg
     7381 cgcttctatg tgattaacgc ggcgaaaatc gcccgcaaat gtggcctggc ggcccgtatt
     7441 aataccgtca tgcagatggc ttttttccat ctgacgcaaa ttctgcctgg cgatagcgcc
     7501 ctcgcagaat tgcagggtgc gattgccaaa agttacagta gcaaaggcca ggatctggtg
     7561 gaacgcaact ggcaggctct ggcgctggcg cgtgaatccg tagaagaagt tccgttgcaa
     7621 ccggtaaatc cgcacagcgc caatcgaccg ccagtggttt ccgatgccgc ccctgatttc
     7681 gtgaaaaccg taaccgcagc gatgctcgca gggcttggtg atgccctccc cgtttcggcg
     7741 ctgccgccag acggcacctg gccgatgggc accactcgct gggaaaaacg caatatcgcc
     7801 gaagagatcc ccatctggaa agaggaactc tgtacccaat gtaaccactg cgtcgcagct
     7861 tgcccacact cagctattcg cgcaaaagtg gtaccacccg aagcgatgga aaacgcccct
     7921 gccagcctgc attcgctgga tgtgaaatcg cgtgatatgc gcgggcagaa atatgtcttg
     7981 caggtggctc cggaagattg caccggttgt aacctgtgcg tcgaagtttg cccggcgaaa
     8041 gaccgtcaga atccagagat taaagccatc aatatgatgt cgcgcctgga acatgtcgaa
     8101 gaagagaaaa tcaattacga tttcttcctc aacctgccag aaatcgaccg tagcaaactg
     8161 gaacgtattg atattcgtac atcgcagctg attacaccgc tgtttgaata ttccggtgct
     8221 tgctccggtt gtggcgagac gccgtatatt aaattgctga ctcagctcta tggcgaccgg
     8281 atgttgatcg ctaacgccac cggctgttct tcaatttatg gcggtaacct gccctctaca
     8341 ccgtatacca ccgatgccaa cggtcgtggg ccggcatggg cgaactctct atttgaagat
     8401 aatgccgaat ttggccttgg tttccgcctg acggtcgatc aacaccgtgt ccgcgtgctg
     8461 cgtctgctgg atcaatttgc cgataaaatc ccggcggaat tactgacggc gttgaaatca
     8521 gacgctacgc cagaggttcg tcgagaacag gtcgcagctt tacgccagca actcaacgat
     8581 gttgccgaag cacatgaact gctacgtgat gcagatgcac tggtggaaaa atcaatctgg
     8641 ctgattggtg gtgatggctg ggcttacgat atcggctttg gcggtctgga tcatgtattg
     8701 agtttgacgg aaaacgtcaa cattctggtg ctggatacgc aatgctattc caacaccggt
     8761 ggtcaggcgt cgaaagcgac accgctgggt gcagtaacta aatttggcga gcacggcaaa
     8821 cgtaaagcgc gtaaagatct tggcgtcagt atgatgatgt acggtcatgt ttatgtggcg
     8881 cagatttctc tcggcgcgca gctgaaccag acggtgaaag cgattcagga agcggaagcg
     8941 tatccgggac catcgctgat cattgcttat agcccgtgtg aagagcatgg ttacgatctg
     9001 gcactcagcc atgaccagat gcgccaactc acagctaccg gcttctggcc gctatatcgc
     9061 ttcgatcctc gtcgtgccga tgaaggcaaa ctgccgctgg cgctggattc acgcccgccg
     9121 tcagaagcac tggaagaaac gttacttcac gagcaacgtt tccgtcgtct gaattcgcag
     9181 cagccagaag tggcagaaca gttatggaaa gatgctgcag ctgatttgca aaaacgctat
     9241 gacttcctgg cacaaatggc cggaaaagcg gaaaaaagca acaccgatta aatgctctgg
     9301 ataaggatta cccaattcta aaaaaaagcc cggacgactg ttcgggcttg tctttttata
     9361 tgttggaaaa tcagtggcaa tgcaatggcc caacagaaaa taaaatctgt gacaataaag
     9421 gcatata
//
LOCUS       DAZPDU010000166         9402 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZPDU010000166 DAZPDU010000000
VERSION     DAZPDU010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9402
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.168"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            206..1072
                     /gene="folD"
                     /locus_tag="ACHURS_003600"
     CDS             206..1072
                     /gene="folD"
                     /locus_tag="ACHURS_003600"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFR1239235.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            1074..1286
                     /gene="ybcJ"
                     /locus_tag="ACHURS_003601"
     CDS             1074..1286
                     /gene="ybcJ"
                     /locus_tag="ACHURS_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFR1239236.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKADGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1394..1915
                     /locus_tag="ACHURS_003602"
     CDS             1394..1915
                     /locus_tag="ACHURS_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR1239237.1"
                     /translation="MPTVITHAAVPLCIGLGLSSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSM
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSCYTTPYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(1951..3336)
                     /gene="cysS"
                     /locus_tag="ACHURS_003603"
     CDS             complement(1951..3336)
                     /gene="cysS"
                     /locus_tag="ACHURS_003603"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFR1239238.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYAADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTEKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3510..4004
                     /gene="ppiB"
                     /locus_tag="ACHURS_003604"
     CDS             3510..4004
                     /gene="ppiB"
                     /locus_tag="ACHURS_003604"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFR1239239.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4007..4729
                     /gene="lpxH"
                     /locus_tag="ACHURS_003605"
     CDS             4007..4729
                     /gene="lpxH"
                     /locus_tag="ACHURS_003605"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFR1239240.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDEPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4847..5356
                     /gene="purE"
                     /locus_tag="ACHURS_003606"
     CDS             4847..5356
                     /gene="purE"
                     /locus_tag="ACHURS_003606"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFR1239241.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGTA"
     gene            5353..6420
                     /gene="purK"
                     /locus_tag="ACHURS_003607"
     CDS             5353..6420
                     /gene="purK"
                     /locus_tag="ACHURS_003607"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFR1239242.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYTLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(6557..7450)
                     /locus_tag="ACHURS_003608"
     CDS             complement(6557..7450)
                     /locus_tag="ACHURS_003608"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFR1239243.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7447..8100)
                     /locus_tag="ACHURS_003609"
     CDS             complement(7447..8100)
                     /locus_tag="ACHURS_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFR1239244.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAAGDPLPAELRQFFAYSDNLQLVTTAVSVSYLR
                     YAAQGYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8111..9370)
                     /locus_tag="ACHURS_003610"
     CDS             complement(8111..9370)
                     /locus_tag="ACHURS_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFR1239245.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETDITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
BASE COUNT         2091 a         2667 c         2493 g         2151 t
ORIGIN      
        1 ttagacaagg aaataagtca gttccagatt tacaacgcca tcatggacga aaaatgaagc
       61 tttcaatctc agcgacggtg cgccccacct tcgcaagagg tcgcttcacg cgataaatct
      121 gaaacgaaac ctgacagcgc gccccgcttc tgacaaaata ggcgcatccc cttcgaccta
      181 cgtaacagat ggaatcctct ctctgatggc agcaaagatt attgacggta aaacgattgc
      241 gcagcaggtg cgctctgaag ttgctcaaaa agttcaggcg cgtattgcag ccggactgcg
      301 ggcaccagga ctggccgttg tgctggtggg tagtaaccct gcatcgcaaa tttatgtcgc
      361 aagcaaacgc aaggcttgtg aagaagtcgg gttcgtctcc cgctcttatg acctcccgga
      421 aaccaccagc gaagcggagc tgttggagct tatcgatgcg ctgaatgccg acaacaccat
      481 cgatggcatt ctggttcaac tgccgttacc ggcgggtatt gataacgtca aagtgctgga
      541 acgtattcac ccggacaaag acgtagacgg tttccatcct tataacgtcg gtcgcctgtg
      601 ccagcgcgcg ccgcgtctgc gcccctgcac ccctcgcggc atcgtcacgc tgcttgagcg
      661 ttacaacatt gataccttcg gcctcaacgc cgtggtgatt ggcgcatcga atatcgtcgg
      721 tcgtccaatg agcatggaac tgttgctcgc aggttgcacc actaccgtga ctcaccgctt
      781 cactaaaaat ctgcgtcacc acgtagaaaa tgccgatcta ctgatcgttg ccgtcggcaa
      841 gccgggcttt attcctggtg actggatcaa agaaggcgca attgtgattg atgtcggcat
      901 caaccgtctg gaaaatggca aagttgtggg cgacgtcgtg tttgaagacg cggctaaacg
      961 cgcctcatac attacgcctg ttccgggcgg cgttggcccg atgacggttg ccacgctgat
     1021 tgaaaacacg ctacaggcgt gcgttgaata tcatgatcca cagggtgagt aacatggcga
     1081 cattttcttt aggtaaacat ccgcacgttg agctgtgcga cttgctgaaa ctggaaggct
     1141 ggagcgaaag cggcgcgcag gcgaaaatcg cgattgccga aggccaggtg aaagccgacg
     1201 gcgcggttga aacgcgcaaa cgctgcaaaa tcgtcgccgg tcagacagtg agttttgctg
     1261 gtcacagcgt acaggttgtt gcctgatccc tttcccggcc tgtatcaaca ggccggttct
     1321 cttttcagac ttttgccctc tacgccccgt tcatccatgc tgatagcatc gacattttgc
     1381 cgaagcgacc atcatgccaa ccgttattac gcacgccgcc gttcccctat gcattggttt
     1441 agggttgagt tcgaaagtca tccctccgcg tctgttattt gccggaatca tcctggcgat
     1501 gctgcccgac gccgacgtat tgtcgtttaa atttggcgtt gcttacggca atgtttttgg
     1561 tcatcgcggg tttacccatt cgctggtgtt tgcgtttgtt gtcccgctgt tatgtgtgct
     1621 gattggacgc cgatggttca gggctgggct gattcgctgc tggctgtttt taaccgtctc
     1681 gttgctatcg cacagcatgc tggattcggt aaccactggc ggtaagggtg ttggctggct
     1741 gtggccgtgg tcagatgaac gctttttcgc tccctggcag gtgattaaag tcgcgccgtt
     1801 tgcattgtct tgttacacca cgccgtacgg gcatcaggtg attatttctg aattgatgtg
     1861 ggtgtggttg ccggggatgc tactgatggg aatgttgtgg tggcgcagac gataaccgga
     1921 tgcgaaaact cgcatccggc aatagcgcaa ttacttacga cgccaggtgg tcccttgcgg
     1981 gccatcttcc agaacgatcc ccatctcgtt aagacggtca cgcgccgcat ctgccgccgc
     2041 ccagtctttc gctttacggg catccagacg ctgttgaatt aacgcttcaa tctcagccac
     2101 ttcgctgtcg tctgcctgcg cgccgctttg caggaacgct tccggttctt gctccagcag
     2161 gcctaataca gcggaaagtt tacgcaggtg agatgccatt gcattcgccg ctgccatatc
     2221 ttctgctttc agacggttta cttcacgcgc catatcaaac agtacggaat aggcttccgg
     2281 ggtgttgaaa tcgtcgtcca tcgcttcaat aaagcgcgct tcaaacgctt cgccaccggc
     2341 aggagcaacg gttttttctg tgccgcgcag ggcagtgtag agacgctcca gcgccgcacg
     2401 cgcctgcttc aggttctctt cgctgtagtt cagctggctg cgatagtggc ccgacatcag
     2461 gaagtaacgc acggtttcag catcgtagta tttcagcaca tcgcgcacgg taaagaagtt
     2521 acccagcgat ttggacatct tctcgcggtc aaccatcacc attccggagt gcatccagta
     2581 gttaacatac tgaccatcat gggcacaggt ggactgcgcg atttcattct cgtggtgcgg
     2641 gaacatcagg tcagaaccgc cgccgtggat atcaaagtgg ttacccagct gcttgcagtt
     2701 cattgccgaa cattcaatgt gccagcctgg acggcccgcg ccccacggag acggccagct
     2761 cggttcgccc tctttcgaca tcttccacag aacgaagtcc atcgggttgc gtttgtcgtc
     2821 gaccacgtca acgcgcgcgc ctgcctgcag ctggtcgaga tcctgacgcg acagcacacc
     2881 ataagtcgga tcggtcggga cgtcgaacat cacgtcgccg ttgtccgccg cataagcgtg
     2941 acctttggcg atcagttgtt cagtgagttc aataatttct gcgatatggt gcgtcgcgcg
     3001 cggctccata tccgggcgca gaatgttcaa agcatcaaaa tctttgtgca tttcggcgat
     3061 catgcgatcc accagcgcca caaagctttc gccattttca ttggcacgtt tgatgatttt
     3121 gtcgtcgata tcggtaatgt tgcgcacata cttcagttta tagccgagga aacgcagata
     3181 gcgcgcaacc acgtcaaaag caacaaaggt acgcccgtga ccgatatgac agagatcgta
     3241 aacggtgatt ccacacacat acatgccgac ttccccggcg tgaataggct taaattcctc
     3301 tttttggcgt gtcagagtat tgaagatttt tagcatcgaa gattccgttt agacatgtgt
     3361 gggtaattga gttgcgtata atacccatat ttcccgcgcg aatcagcata cattgcgtga
     3421 tgatccgatc gtgcggttat gctataacac caccctatat atgacccgaa ctgggttgaa
     3481 gcaccaatca aacggaacag gatgcaaaaa tggttacttt ccacaccaat cacggcgata
     3541 ttgtcatcaa aacttttgac gataaagcac ctgaaacagt taaaaacttc ctggactact
     3601 gccgcgaagg tttttacaac aacaccattt tccaccgtgt tatcaacggc tttatgattc
     3661 agggcggcgg ttttgaaccg ggcatgaaac aaaaagccac caaagaaccg atcaaaaacg
     3721 aagccaacaa cggtctgaaa aatacccgtg gtacgctggc aatggcccgt actcaggctc
     3781 cgcactctgc aactgcacag ttcttcatca acgtggttga taacgacttc ctgaacttct
     3841 ctggcgaaag cctgcaaggt tggggctact gcgtgtttgc tgaagtggtt gaaggcatgg
     3901 acgtggtaga caaaatcaaa ggtgttgcaa ccggtcgtag cggtatgcac caggacgtgc
     3961 caaaagaaga cgttatcatt gaaagcgtga ccgttagcga gtaatcgtgg cgacactctt
     4021 tattgcagat cttcatctct gcgtggaaga accggcgatc accgccggtt ttctgcgttt
     4081 tttagcgggg gaagctcgca aggccgacgc gctgtatatt cttggcgatc tgtttgaagc
     4141 gtggattggc gacgacgagc ccaatccact ccatcgccag atggcggcag cgatcaaagc
     4201 ggtgtccgat tctggcgttc cctgttattt cattcatggc aaccgtgatt ttctgctcgg
     4261 caaacgcttt gcccgtgaaa gtggcatgac gttattgccg gaagaaaagg tgctcgaact
     4321 ttatggccgc cgggtgttga ttatgcatgg cgacacgctg tgcaccgatg acgcgggtta
     4381 tcaggctttt cgcgccaaag tccacaaacc ctggctgcaa atgctattcc tcgccctgcc
     4441 gttgtttgtg cgcaaacgca ttgccgcgcg aatgcgagcg aacagtaaag aagccaacag
     4501 cagtaaatcg ctggcgatca tggacgttaa ccaaaacgcg gtagtcagtg cgatggaaaa
     4561 acatcaggtg caatggctga tccacggaca tacccatcgc ccggcggtac atgaacttat
     4621 cgccaaccag caacctgctt ttcgcgtggt actcggtgcc tggcatacgg aaggttcgat
     4681 ggtgaaagtc acggcggatg acgttgagct gattcatttt ccgttttaaa aaaaccgcaa
     4741 ctttgctgat ttcacagcca cgcaaccgtt ttccttgctc tctttccgtg ctattctctg
     4801 tgccctctaa agccgagagt tgtgcaccac aggagtttta agacgcatgt cttcccgcaa
     4861 taatccggcg cgtgtcgcca tcgtgatggg gtccaaaagc gactgggcta ccatgcagtt
     4921 cgccgccgaa atcttcgaaa tcctgaatgt cccgcaccac gttgaagtgg tttctgctca
     4981 ccgcaccccc gataaactgt tcagcttcgc cgaaagcgcc gaagagaacg gttatcaggt
     5041 gattattgcg ggcgcaggtg gcgcagcgca cctgccaggc atgattgccg ccaaaacgct
     5101 ggtgccggtg ctgggcgtgc cagtacagag cgccgcactg agcggtgtcg atagcctcta
     5161 ctccatcgta caaatgccgc gcggcattcc ggtgggtacg ctggcgattg gtaaagctgg
     5221 cgcggcaaac gcggcattac tggcagcaca aattctcgcg actcacgata aagagctaca
     5281 ccagcgtctg aatgactggc gcaaagcaca aactgatgaa gtgctggaaa acccggaccc
     5341 gcgaggtacg gcatgaaaca ggtttgcgtc ctcggtaacg ggcagttagg ccgtatgctg
     5401 cgtcaggcag gcgaaccgtt aggcattgct gtctggccag tcgggctgga cgctgaaccg
     5461 gcggcggtgc cttttcaaca aagcgtgatt accgctgaga tcgaacgctg gccggaaacc
     5521 gcattaaccc gcgagctggc gcgccatccg gcctttgtga accgcgatgt gttcccgatt
     5581 attgctgacc gtctgactca gaagcagctt ttcgataagc tccacctgcc gaccgcgccg
     5641 tggcagttac ttgccgatcg cagcgagtgg cctgcggtgt ttgatcgttt aggtgaactg
     5701 gcgattgtta agcgtcgcac tggtggctat gacggtcgcg gtcaatggcg tttacgcgcc
     5761 aatgaaaccg aacagttacc ggcagagtgt tacggcgaat gtattgtcga gcagggcatt
     5821 aacttctctg gtgaagtgtc gctggttggc gcgcgcggct ttgatggcag caccgtgttt
     5881 tatacgctga cgcataacct gcatcaggac ggtattttgc gcaccagcgt cgcttttccg
     5941 caggccaacg cacagcagca ggcgcaagcc gaagagatgc tgtcggcgat tatgcaggag
     6001 ctgggctatg tgggcgtgat ggcgatggag tgttttgtca ccccgcaagg tctgctgatc
     6061 aacgaactgg ctccgcgtgt gcataacagc ggtcactgga cacaaaatgg tgccagcatc
     6121 agccagtttg agctgcatct gcgggcgatt accgatctgc cgttaccgca accagtagtg
     6181 aataatccgt cggtgatgat caatctgatt ggtagcgatg tgaattatga ctggctgaaa
     6241 ttgccgctgg tgcatctgca ctggtacgac aaagaagtcc gtccggggcg taaagtgggg
     6301 catctgaatt tgaccgacag cgacacatcg cgtctgactg cgacgctgga agccttaatc
     6361 ccgctgctgc cgccagagta tgccagcggc gtgatttggg cgcagagtaa gttcagttaa
     6421 gcaggtgctc atttgattgc cggatgcggc gtaaacgcct tatccggctt accgctccgg
     6481 tacccattgt aggcctgata agacgcgtca agcgtcgcat caggcatggt gcaccgattg
     6541 ccggatgctc tcatcactac agcgaaatac aggtccccgc ttcgcccgcc agcgtctctt
     6601 caattcgcga taacgcccca atccacgcgg gtttaccacg gcttctgaca taaccactca
     6661 ccgccgttac cttcggcccc atcgaaccat cggctttggc aaatggcgct aactcatccg
     6721 gtgtggcatg gcgaatggca cgttgctgcg gcgttcccca gttttcatat accgcatcag
     6781 catcggtgag gatcaccagt ccatcggcat taatctgctc ggcgagcaac gcagcggcga
     6841 gatctttatc aatcactgct tcactccctg ccccatcttc cgtcacaggc acacctccgc
     6901 cgccactgca aatcaccaca tgcccctctt tgagcaacaa ctcgatggct tcgctgtcga
     6961 gaattttacg cggttgcgga gacgccacca cccggcgcaa atatttacca tcacgtttca
     7021 tctgccagcc gtaagccgct tccagtgcct cttgttcttc tggctgataa accggaccaa
     7081 taaatttctc tggctgcaaa aacgccggat catcaggcga aacctcaatg cgcgtcagca
     7141 ccgtcgtaac aggcggcatc tgcggctgtg cgctcaagct ctgcgccagc atatagccaa
     7201 tcatcccctg gctttccgcc accagcacat ccagcggata cggttctacc tctttccacg
     7261 ccagattctg taacgccagc agccccacct gcggcccgtt gccgtgaaca atcgccagcc
     7321 gataagaacg ggccaggcgt gccagcgcgg gtacagcact ggcgatattg cgatattgat
     7381 tttccgccgt cagcgcctca ccgcgctgga gtaaggcgtt gcccccaaga gctacaacca
     7441 gtgttttcat ggttttcctt gtaataattg ttggccaagc cagaacccca gcagcgtatc
     7501 tgcccctgac gtatgcccca gcgccagcag cgaatcaatc gcaacagcgg tacgtttcgg
     7561 gcaactcaga gcatggacaa agtgcaggag tggcgaggcg aaatatcctt gcgcggcata
     7621 acgtaaataa ctgacgctca ccgctgtggt aacgagttga agattgtcgg aataggcaaa
     7681 aaactggcgt aactcagcag gcagcggatc gcctgccgcc aacgccagtg gcccgaaaag
     7741 cccggtttcc tccgcgcgtt gcatccatgc agctgcaagc ggttgaggat gcgcaggcgg
     7801 cgtaatacgc agcaaacaat aacgctgtgg ctgtttaacc gtgaaacgcc cgaggcgaat
     7861 cccttgagcc acaacctgtg gtcgttcatt gccgcataat ccaccgcata acgcatcgaa
     7921 ttgcgcgcgg cgaagcaccc atcctccggg gccgaaacca ctaccctgac gatgcagcgt
     7981 taacagttcg ccgctttccg tcatcaggtt aatcgcccgt cgccacactc ccgctaaccg
     8041 ccaggactgg cgataattcg gtgcactacg gctggcaagc agaggatgga tgatcgtcat
     8101 tgcgcgttcc tcaaccaatc cccatgcttt cagccagtgc ttccagcgcc tgttcaaaac
     8161 acgccagcgg tgcccgcaca gtgcctgcgc caatctgccc gatccccgcc tctttatggg
     8221 cgataccggt attgatgagt ggcgtaatgt cggtttctac cacgcgacga atgtccagtc
     8281 ccaggcacgc gccctgaaaa tcccagcctg ggatctgcaa ctgcatattg cgctcaaggt
     8341 aaatttccgc catctcttca gataccgctc tggccgcttc catgccaccc gcaccgacaa
     8401 agcgcgttac gccaggcgct gcgatcattg ccgcacctcc gataccaaag gtttcggtaa
     8461 tcgcgctatc gcccatatcc gggttcgcct gctcctgcga gaagccggtg aaaaacagac
     8521 cttgcggagt gtttacaggc gcagtaaacc agcgttcgcc cagcccgctt acccgaatcc
     8581 cgaacatatt gccgttgcgg gtcattgccg tgacgatgct gcctgcgcgg atcatcgcgc
     8641 cagcatccat cgccgccttg cagtaagcca tcgcgaggtt gaggaagaac tgatcggtca
     8701 cgctgaggaa atccatcact tcggcgatgt gctgtttatc atgatcgagg cgagcaattt
     8761 gtggggccag cgcacgcatt aacagtgcag aggaagcaat attgcgttga tggaactcat
     8821 cgcccatcgt aatgccctgc gccatcatcg ccgtgagatc gataccgcgc tccatgcgcc
     8881 ccagcgccgc gcttaatact ggcattaaca catcgcgcat ccagcgatgg cgagtcagga
     8941 catcttcgcc gtaagcgcca aaacgcatca ctttgccgat accttcgttg aggttgcagt
     9001 acgcccggtt gccgtcggtc acgttctcga ccaccagcat cggcatactg gcagaagtaa
     9061 taccgcccat tggaccgacg gcattcacat ggtgacaagg aatgaagttc acttccccct
     9121 gctccagtat tgccagcgcc tgcgcttcat ctttcgccca gccttcgaac agacatgcgc
     9181 ccacgcacgc ccctttcatg ggtccggtca tctcctgcca gcgcattggc ggcccggcgt
     9241 gaagcagtgt tttgccctcg tttagttcgc tgataagtga agaagccggt tgcacatcca
     9301 gccagtgtgg acgagcgcga cgaatttgtt cgataaccgc agcattggct tgcgccactg
     9361 atgtaaacat gggaacccct tattgcaaac gttctaataa ac
//
LOCUS       DAZPDU010000167         9075 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZPDU010000167 DAZPDU010000000
VERSION     DAZPDU010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9075
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.169"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(507..1274)
                     /gene="fecE"
                     /locus_tag="ACHURS_003611"
     CDS             complement(507..1274)
                     /gene="fecE"
                     /locus_tag="ACHURS_003611"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HFR1239246.1"
                     /translation="MTLRTENLTVNYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(1275..2231)
                     /gene="fecD"
                     /locus_tag="ACHURS_003612"
     CDS             complement(1275..2231)
                     /gene="fecD"
                     /locus_tag="ACHURS_003612"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HFR1239247.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPYTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(2228..3226)
                     /gene="fecC"
                     /locus_tag="ACHURS_003613"
     CDS             complement(2228..3226)
                     /gene="fecC"
                     /locus_tag="ACHURS_003613"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HFR1239248.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(3223..4125)
                     /gene="fecB"
                     /locus_tag="ACHURS_003614"
     CDS             complement(3223..4125)
                     /gene="fecB"
                     /locus_tag="ACHURS_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HFR1239249.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIASVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(4170..6494)
                     /gene="fecA"
                     /locus_tag="ACHURS_003615"
     CDS             complement(4170..6494)
                     /gene="fecA"
                     /locus_tag="ACHURS_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent Fe(3+) dicitrate receptor FecA"
                     /protein_id="HFR1239250.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWMF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVVYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGALKF"
     gene            complement(6581..7534)
                     /gene="fecR"
                     /locus_tag="ACHURS_003616"
     CDS             complement(6581..7534)
                     /gene="fecR"
                     /locus_tag="ACHURS_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric citrate uptake sigma factor regulator
                     FecR"
                     /protein_id="HFR1239251.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADMRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(7531..8052)
                     /gene="fecI"
                     /locus_tag="ACHURS_003617"
     CDS             complement(7531..8052)
                     /gene="fecI"
                     /locus_tag="ACHURS_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HFR1239252.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            8189..8257
                     /gene="ykiE"
                     /locus_tag="ACHURS_003618"
     CDS             8189..8257
                     /gene="ykiE"
                     /locus_tag="ACHURS_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkiE"
                     /protein_id="HFR1239253.1"
                     /translation="MGWSLCFWHVSVRMTHDVVCYY"
     gene            8344..9041
                     /locus_tag="ACHURS_003619"
                     /pseudo
     CDS             8344..9041
                     /locus_tag="ACHURS_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1F family transposase"
BASE COUNT         1988 a         2511 c         2585 g         1991 t
ORIGIN      
        1 acacttttta gaaaaatcga tggtcagaaa ctggattagc aattccgttc cagggttgct
       61 tttgatttac gttggcgtct gatcattgat ttatcctcaa aagcccaacc tcattggtaa
      121 tgaaccagct ccgtgaatgt ccgctctggc acagagcgaa attttttgat ctcccccccc
      181 tgaaatctaa acttagtcat gtcacgtttt tgggtttcta aaattttaac ttcgcgtttt
      241 tcgttgccgt aagggttata cagaaatgtc cgttaagcag agttcaaaat tgattgccgt
      301 gatcacgact ggtttgaaag ccgcgcccaa gcctgtacag ctctggtttg cgttgattat
      361 gaacctgtca gcctaaagca agcggatgga cgatgagtat tggtaatctt tcagagtccg
      421 gaaaagttca gccccagtct gaacaggctt gctggcgcca gtccagtttc attcagtcgt
      481 ggtttggttc ttacggcctg tgcaatctac ctcattaggc acatcggcct gccagatacc
      541 ggctcggggt gtatttccgc ttccacgctg aatactgttc tcagcaatcc tggggtcatc
      601 acctcttctg gtgtgccttg cgccataaca tgtccgtttg ccattaccac cagttgatcg
      661 cagtaccggc tagcctgatt aaggtcgtgc agcacagcga ccaccgtttt cccctgagtc
      721 cggagttcgc ccatcaaccg catcaggtcc acctggtgat tgatatcaag ataggtggtt
      781 ggctcatcaa gtaatacaac gggcgtattc tgggccagga ccattgccag aaatgcgcgc
      841 tggcgctgac cgccggaaag ctcggttaac cgacgaacgg caagatgatt gatccgggtc
      901 tggttcatgg cgacattaac tcgtgcattg tcttcagcgg agagacgccc ccagagtgac
      961 agccagggat tacgaccata cgaaaccagc tcctggactg tgatcccctc tggcgttaaa
     1021 tggtgctgag gcagcagcga aagcctgcgg gccaactggc gcgatgagag catatttatg
     1081 ggattatcgc cgagaaatac ggtgccagac tgcggcatta aaagccgcga aaaacagttt
     1141 aacagcgtcg atttcccgca accgttagga ccgatcaggg cggtgatctt ccccgttggc
     1201 agtgagagtg aaacgtcgtt aagtaccttg tctgtcccgt aattgaccgt cagattttca
     1261 gttcgtaaag tcatttatcg cattctcaca agcaaccaga caaaccacgg cgcaccgata
     1321 atggcggtca gcacgccaac cgggagctcc agtgggggat gaataattct cgccagcaga
     1381 tcggcaacca ccaacagcaa cgcacctgtc agggccgaaa caggcagcag tctgcggtga
     1441 cgtccaccgg tgatgctacg catcatatgc ggcaccacga gaccaataaa gctaatcggg
     1501 ccgcaggcgg ccacgccggt agatgtcatg gcgacagcta gtaacaaagc ccagaatcgg
     1561 gtatagggca ccgacacacc gagcgtggtg gcgcgcgcat cgccgagtgc aaggaggtcg
     1621 agatcgcggc aaaaactcag gctcagcggc agaaataaaa tcatcagcgg gatggcaatc
     1681 ttcacaaagc tccagtcacg gccccataag ctgccggtca gccacagcag ggcgttgttc
     1741 acatcctgcg ggcgcgagag catcagataa tccgtcaggc tggcccagca tgcagaaagc
     1801 gccacgccgg tgagtgccag cttcatcggc tggtgggtct ttgccagcat cttcagcaat
     1861 atcaaccccg ccatgccgcc cgcaaaggcc agcagcggca gcaccatcac gggcagtgac
     1921 ggcataagaa gtagagcccc cacagaggcc aggctggcgg catggttaac gccgagaata
     1981 tccggtgatg ccagagggtt gcgcacaatc ccctgtatca gcacgcccgc cacggcgagg
     2041 gctgcaccga caaacagtgc cagcagcaag cgcggcagtc ggtactccat caatacataa
     2101 taatgctcgc gtccggcctg ccagtcggtc agcagcgcgc gccacggcac ggggatcact
     2161 cccatatgga gtgataacag cgcacagccc gccagggcaa gggtgatgaa aataaccagc
     2221 gcaattttca tcctcgcctc ctcacaagcc agacaaagca agggctgcca atcagcgcca
     2281 gcactgcgcc tgcgggcaga tctccgggga aggccagcgc gcgtgcgagt acatctgcca
     2341 gcagcatcag cgtggccccc agcagcatgc tcactggcag tacgttgcgc tgatcgaagc
     2401 ctgcccagaa gcgcgccaga tgtggcacca gcagaccgat aaacgccacc ggacctgcca
     2461 cgctgacgca cgcaccaacc agaagcagca ctaacatatt gatgaccaaa cgtagcctcg
     2521 tcaggttcac tcccagcgta tgggcggtgc tgtcgctgag gttgagcagg ttcagttgat
     2581 tcgccagcag caacacgaca gggactgcag tgaccaccac cggcaagagc tgccagacat
     2641 cctgccagcg ggcgtgggac actccgccag ccagccagta aaagatgccg taagcatgat
     2701 cttcggccag cagcagggtg atgcgggtaa ggcccataca aaaggccgac agcgcgatac
     2761 ccgcgaggat cagtttgttt ctgtcatggg tatgacgaaa tccgcctcct gcggtcatga
     2821 ccagcagcca gctcacgccg cccccgcatg ccgcgatgaa cgacagagaa tagcctgcaa
     2881 tcggcgtcgg actcagcgcg ctggtaagcg ccatagccag cgccgcgccg ctgttaatgc
     2941 cgagcagtga aggagaggcc attgggttgt gggtcagggt ttgcagcagc gtgcccgcga
     3001 gcgccaggct tgcgccgatc agaacggcga ccaggcttcg tggcaaacga aggttttgca
     3061 ccagcgcttc tggtagcgtt ggcgtgtgtc caggcagcag ggcgcgggtt gcatctgctc
     3121 cggaaacagg aatggccgag tagcaaaaca gactcagcca gaaaataata ataagtgctg
     3181 caacgggaag cccccacagc agcaccgggt gttttatcgc ggtcatttca caacggtaag
     3241 cggctgatgg tggaagattt ttaccgtgtc agcggcaata cgctctgcag caaaaatacc
     3301 gcgcatccgc gcccaggtgt tactgtcgac cgaagcaacc tgctgcttct gcgcggcggt
     3361 taacatctgc cagagcggat cttgttgcca gcgtttaaca atgctctctt cgcgatagtg
     3421 ggcaaccagc agccaggcag gattgaccgc cagcagttgc tccaggccga tggacggcat
     3481 ggacgcaccc gccatcgcag cgggaacgtt cagccccaga gaggccagca cgctgccggt
     3541 ccaggtctcc tgagtatgca ggttgaattg ctgttcgcgt gatgtgccaa aggccacgcg
     3601 tgtccctttg ggaagctggc tggcccactg cgccatcctc tctttatgtt gttccagacg
     3661 tgcctgcatc tctcgctttt tacccaccat ttcgccgatg atagccgcag attgcaaatt
     3721 ttcagcgtag gtttcgttgc gggacttaag cagcagtacc gacgcgattt gctgcaaggc
     3781 gatgtaaacc cccgcatggc gactgctgtc ggcaatgatc aggtctggtt tcagagcggc
     3841 aatggcttcc aggctcggct gcgcgcgcgt tccgacggac tgccacggtt tcaggtgcgc
     3901 acgcacttcg ggcaggatgc gttttgcatc gttatcgtcg gcaataccga tcgggctgac
     3961 gtccacggcg gccagcgcat cggcgaacga gagttccagc accacaatcc gttgtggcgt
     4021 tttttcgagt gtaaacgtgc cgtgttcgtc ctgaaccgtg gcggcaaagg cgtggctgat
     4081 caccagcagc aggcctgcaa aaagaaaacg gataaatgcc aacataatca cattccagct
     4141 aaaagcccgg caagccgggc gttaacacat cagaacttca acgccccctg catatacagc
     4201 gtgcgcggct ggcctgcata gatgcctttg ttgttgtcgt cataagagcg gatgaagtag
     4261 tcctggtcga agatgttttt cacaccgaat gccaggttca gatctgccat ctgcgggcca
     4321 aagtcataca ccacgcgtgc gccccagagc atgaagccgg gaatgcggcc ggtactgccg
     4381 tcggcgctct ctttcaccgt attggcgtta tccgcaaact ggctggactg gaaatcgctg
     4441 ttcagattga acatccagtt tcctggcttg tagtccacgc ccagcgtgcc tttatgtttc
     4501 ggggagaatg gtaccagatt gccgtaggtg tcgcctttct cgcggatttc cgcgttcaca
     4561 tacgcatagc tggcgtagat ggaaacgtta tcaagcgttg gcgttagcgt acccagatcg
     4621 taacgtgcct gcgtttccag cccggtatgg cgcgttttgc cacgtgcagt gacggtgtcg
     4681 ttggtctggt tggagtcgta ctgattgtta aagttaatca ggaacagccc catttccgcc
     4741 gtcagcgcgc cgtcgtcgta gcgggtaccg agttcccagg ttcgcgcttt ttccggttcc
     4801 acattgccgc tttgcacagc cttgccaatc tggctgtact gtacggtgcc gaacgagcct
     4861 tcagtgtttg cataaagatt ccagctgtca gtcaggtgat agagcacgtt caacgccgga
     4921 agcggtgcgt tatagctcac ttcttcgtgc gtgcctgtga tggcgttgtt ctggtatgac
     4981 tcgatatgtt cgaaacgcat acccggcgtg atggtccagt tgccgatgtc gattttgtca
     5041 tccagatacc aggcgtgcgc ctcggtgccg gaacgcgtat cgcggtcgta agggcttgag
     5101 ccggacggca actgcccgct gctggtggcg gtgtagtaac gcatttcatg cgttgattca
     5161 ttcacatagc gatagcccac gcccacttcg tgcgcggaag ggccgatcat aaagatctgg
     5221 ctgtagcgtg gctcaatacc gcgcacccag tagttacgcg gcgagagggt gatgcgtttg
     5281 ccttgctcca ggtagccgct gcgcagggtt tgggtgtaga acccctgaat gttgaattta
     5341 tgctggctgt ctggctggaa ctggtagccc aggctcgcca gcttgcgacg accccagaag
     5401 cggtcatacg ggcgggtgga ttgccagcga tcggcgtcgt aatccgcgcg agacaggcca
     5461 ccgggcatgt cggcttcacc gtcgtaatat tgcagcaggc tgttgaaggt gtgcacctca
     5521 tccggcgcat atttgctttt cagcatcagg tcgtcgatgc gggtggcgct gtgctcgcgc
     5581 cagtcactgc cgcgcgtgcc ggagtagagc agcgcggtgc caaaaccgtt gtccgctgtg
     5641 ccgcccacca tcaggttgtg cgtctctttc gggttgtttt gtgaagaggt tgggctgagc
     5701 tgaccttcca cgcccgcctc gataccaaag tcctgcggaa tggcacgggt aacaaagttc
     5761 accacgccgc ccacgctctg cggtccgtaa cgcaccgcac caccaccgcg taccacgtca
     5821 atggcatcca tgttgccgag cgaaacggga gccagtgaaa gctgcggctg accgtaaggg
     5881 gcgaagggga cggggatgcc gtccatcagg acggtcgagc ggctggcgag gcgcgggttc
     5941 aggccccgga tgccaaagtt catcgccagg tcgtggctgc cggtgccgtt gttttccggc
     6001 gcgctgacgc cagggatgcg gttaagtacc tcacgcatgg tggttgcgcc ggttttggcg
     6061 aaatcctcac ggcggatcac gtcacgcgcg ccagcatgtt caaatacgtc gttttcacgc
     6121 gcatcaccca gccagtcgcc gaccacggtc agggcatctt cttttggtgc gggcgcgggc
     6181 tccagcgtcc agctgttatt tcccagcggt tttacctgca gtccgctgcc gtccagcagt
     6241 tgttgcaggc cgctctcgac gtcgtaatcg ccgtgcaggc cgttgctctg cttgccgcgc
     6301 gtcaggctgg cgtcaaccga gagggtaaat ccgctgtgtg cggcatactg attgagcgct
     6361 ttatcgagcg atcccggtgc gatattaacc tgtgcagcaa aagcggaaaa cgagagaccg
     6421 gccagcggca gcaggctcag gcgaatggcg ttaaccaaag gtgttgtttt acgaaaaacg
     6481 cgtaacggcg tcataccttc cccatcatca tttttgttgt gttcagctat gagtcgaacg
     6541 agaagggtaa aaaggacaat cgaaataaga attattttcc ttacagtggt gaaatgttta
     6601 tccagtaccg cgtaatagac tgaattttaa cgggaagcgt ttgcgcgata acgttcagga
     6661 tcgcatcggt atttttcagc gggaacgtcc cgctcaggcg cagcccggca acggcgggat
     6721 cgcagcgcag cacgccgttg cggtaacggg ttagcgtggc tatcacctca cccagcggtt
     6781 tatcgctgaa gctcaggatg tccttcgtcc agcttgtact ctcgtcatcc agcggtttca
     6841 ctgcgccaaa ctcagaggcg ctgaactgca ggctttcacc agcgttcacg atgcgttttt
     6901 gcgcgggggc actggcgaga agcacttcca cggcgtgctg ctgcacgtca agctgcgtga
     6961 aattatcctg ctggcggacg gtaaattctg tccctaaagc agtgagctgg ccctgacggg
     7021 tcaggacacg gaaggggcgt tgcagggcat ctttcgcggt agtaatggcg atttcaccgt
     7081 accagagccg gacggtgcgc tgatgcgcat caaaacgcat atccgccgcg ctttgggtat
     7141 tcagcgtgag cagggagcca tcttccagtt gctgacggct gactgtgcct ttggcggtgc
     7201 ggtaatctgc ccgcagacct tcgccggttt ccgactgcca gagctgccag cctccgccag
     7261 cgccgagcaa cagcagtaat cctttcatca cgtgacggcg ggtgaggcgg gtatcgtgca
     7321 acgcccggct ggcaacgtcg ccaggcacac cgccaagctg gttgcgcagg ttttcaacct
     7381 gctgccaggc ccactggtta tcctgatcct gttcatacca ctgttgccag cgcgcttcct
     7441 gttgtgggct gacgcgctcg ccgcttagca cggcatacca gtgggaagct gaacgcagcg
     7501 cctgacggcg ggaatcggtt aacaaaggat tcataaccca tactccagac ggaacagcag
     7561 gcagtgctcg acggctttcg ccacgtattt tttcacggag ctgatggaaa caccgagttt
     7621 gtgcgcaatc tcgctgtatg tcagaccatc cagttgcgaa agcagaaacg cttcacgtgt
     7681 tttgccgttt agcccgtcca gcatgctgtc gaggagttgt agggtctcga gttggctttc
     7741 gcgttcctca ggtgaaggcg ctcccccctc cggcataagc gccagcatct ccagatacgc
     7801 tttttccagc gcgtttcggc gaaacaggtc caccatcacg cgtttggcga tagtgcagag
     7861 gaaggagcga ggatcgcgga tcgtcgagag cgtttcgctg accattaccc gcaaaaaagt
     7921 gtcctgggca atgtcatctg catcaaaagc agactggagt ttgcgcgtca gccagctttt
     7981 caaccagccg tgatgtgtgc cataaagcga ctcgaatgtt aaggaagctg tggtagtggc
     8041 gcggtcagac atgcggagtg catcaaaagt taattatcac gtagccatat taatatgaga
     8101 atagttatca ttacaattgg aaataaaatt gtttccaata gacattttta acatgttgtt
     8161 tttctaagtg ttataaggta ggtataaaat gggatggagc ctctgcttct ggcatgtgtc
     8221 ggtcagaatg actcatgatg tggtctgcta ttattgacat cctcactgcc ctaaaggata
     8281 gggatttcgg taatgctgtc aacttactga tttagtgtat gatggtgatt ttaaggtgct
     8341 tgcgtggctt ccatttccat cagatgtcct tcctgctccg ctactgaagg cgtggtgcgt
     8401 aacggcaaaa gcactgccgg acatcagcgc tatctctgct ctcattgccg taaaacatgg
     8461 caactacagt tcacttacac cgcctctcag cccggtaagc accagaaaat cattgatatg
     8521 gccatgaatg gcgtcggatg tcgcgccagt gcacgcatta tgggcgttgg cctcaacacg
     8581 gttttacgtc acttaaaaaa ctcaggccgc agtcggtaac ctcgcgcata caaccgggca
     8641 gtgatgtgat tgtctgcgct gaaatggacg aacagtgggg ctacgtcggt gctaaatcac
     8701 gtcagcgctg gctgttttac gcgtatgaca ggatacggag gacggttgtg gcgcacgtct
     8761 tcggtgaacg cactctggcc acactggagc gtcttctgag cctgctgtcg gcctttgagg
     8821 tcgtggtata gatgacggat ggctggccgc tgtatgaatc acgcctgaag ggaaagctgc
     8881 acgttatcag caagcgttac actcagcgca ttgagcgaca taatctgaat ctgagacaac
     8941 atctggcaag gctgggacgg aagtcactgt cgttctcaaa atcggtggag ctgcatgaca
     9001 aggtcatcgg gcattatctg aacataaaac actatcagta agttggagtc attacctcct
     9061 cccgaactca aaact
//
LOCUS       DAZPDU010000168         8726 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZPDU010000168 DAZPDU010000000
VERSION     DAZPDU010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8726
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.170"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..1782)
                     /gene="atoS"
                     /locus_tag="ACHURS_003620"
     CDS             complement(<1..1782)
                     /gene="atoS"
                     /locus_tag="ACHURS_003620"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HFR1239254.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGDRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKMLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSML
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTADLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTSDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIQTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATF"
     gene            1949..4798
                     /gene="rcsC"
                     /locus_tag="ACHURS_003621"
     CDS             1949..4798
                     /gene="rcsC"
                     /locus_tag="ACHURS_003621"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFR1239255.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(4884..5534)
                     /gene="rcsB"
                     /locus_tag="ACHURS_003622"
     CDS             complement(4884..5534)
                     /gene="rcsB"
                     /locus_tag="ACHURS_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFR1239256.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(5551..8223)
                     /gene="rcsD"
                     /locus_tag="ACHURS_003623"
     CDS             complement(5551..8223)
                     /gene="rcsD"
                     /locus_tag="ACHURS_003623"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFR1239257.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDTTATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAAGPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         2137 a         2026 c         2231 g         2332 t
ORIGIN      
        1 gaaggttgcg ccgtagcccg gcaaactggc gacgcgaata tcaccctgat gggcattaat
       61 gatgcgttga cttaacgcca gaccaagccc ggttcctgag gctttggtgg tgaaaaaggg
      121 atcgaagatc tttttttgca gcgagagatc aatgccacag ccgttgtcct ctatcgaaat
      181 ggcctgttgt gagtcgctgt attgccaggt ttgaatgcga attttccctc gtgcgctgat
      241 agcctggacg gcattgatca ggatattcag tagtacctgt ttgagcagtt cacgatcggc
      301 gttaatcggg cttaattcat tatccagttc gcttatgaag tcgacccgcg cttgtacgcc
      361 ggcggtttgt accagtacca gagtttcttc aaccaatgca ttgaggctga cttgttgcca
      421 ttgactgtgg cgtggacgtg aaaattcgag caattgctga ataactttgt taattgaatc
      481 gatttctttg agtactacgg acagatattc ctgatgtatt gggtcactgg tttgttggcg
      541 caagatctgt acataaccac gaatagccgt taacggatta cgtacttcat gcgcgacgcc
      601 agccatcagc tcacccagtg tggcgaggcg ttctgcttgc gccatgcggc gctgggtttc
      661 tttgcgggca gttaaatcag agaaaatcac caaagcacct atcatttcac cgtgcgtgtt
      721 atgaatacga ctggtagtga cactgagttc aatggtgcgg tcacgacctg gaaaactgat
      781 ctccagcgcc acatgttcgg tgccatgttc cagcgtatcc agtactggac tgtagaactg
      841 agtattgtcg aacaacatgg agtaaggctg ccctaccagt tcatggcgtt gatagccagt
      901 gataacttct gctgctgggt tcatggtggt tacatcaccc tggcggtcaa tggcaatgac
      961 gccatcggca gcgttttcaa taatcagatc gttaagtgtc cgcgtttcac gcagtgcctg
     1021 ggcgaggtta ttaacactct gactgatttg ccccatttca ccgggcaatt gtggtaatcg
     1081 agtgggaata ttttgtgcca gagtcgagag gccatcggtg atgatatcaa tattggcgct
     1141 caggcgacgg gagaaaagga caatcaatag caggcttatc agcaaatcgg cggtgagcac
     1201 aatgataatc ctcacatcca ttttccaggc ctggcggcga atatcttcgg ttaattcatt
     1261 ggcccagata tagccgagga tttcaccatt acgctcaatg gggagcattg aattcaaaat
     1321 atcgccgcgc acctgcctgc ctgaataaac caaaggggta tttgtacgca tgacttcgcg
     1381 accagggtga tctgcggcaa tggtaacgcc gacattattc tgatatagcg ctgaaggcgc
     1441 gtaggtgatt atcgcatcca gcattttgtt gtaataacca gcacctatgc cagggaaggc
     1501 gtgagtgata ttttcggtaa tgggggcaag ttctgcattt aatgcgcgga tacgctcctc
     1561 acgtggtaag tcgatgtaga gatcatagcg atcgcctagt gcctgattaa gcaggttgac
     1621 cacggcagaa agttttttct ctttttcaga taagactgct gaacgtcctt ccgtttctac
     1681 gatataacca atagtaagcg ttgggacaat gaccatcagg attgccatca ggatcatttg
     1741 attgcgtaag cggcgtggat aaatccactt catataatgc atgctgaaat ccactagtct
     1801 tgtccggtat atgacgatta tcagaggtta aggtgatgat ttctcggcgg tgtatcatat
     1861 tccagagaag agagaacatt gcggtaacac gcttttaccg ctaccttaac cacactccat
     1921 cggtcacctg aggcggagct tcgccccttt gaaatacctt gcttcttttc gtacaaccct
     1981 gaaagcctcg cgctacatgt tcagagcatt ggcgttagtg ctctggctgt tgattgcttt
     2041 ttcatccgtt ttttacatcg ttaatgcgtt acatcagcga gaatcggaaa ttcgtcagga
     2101 atttaatctg agttccgatc aggctcagcg ctttattcaa cgcacctctg atgtgatgaa
     2161 agagctgaag tacatcgccg aaaatcgctt atcggcagaa aacggtgtgc tttccccgcg
     2221 tggacgagaa acgcaggcgg atgtgcctgc gtttgaaccg ctgtttgccg actccgattg
     2281 ttccgcaatg agtaacacct ggcgaggttc tctggagtca ttggcgtggt ttatgcgcta
     2341 ctggcgcgat aatttttctg cggcttacga tctaaaccgg gtatttttaa tcggcagcga
     2401 taacctctgc atggccaatt tcggtctgcg tgatatgcca gtggaacgcg ataccgcgtt
     2461 gaaagctttg catgaacgca tcaataaata tcgaaatgca ccacaagatg atagcggcag
     2521 taacctctac tggatcagcg aaggtccgcg ccctggcgtc gggtattttt acgcgttgac
     2581 gccagtttat ctggcgaacc ggttgcaggc gcttttgggt gtcgagcaga ccatccggat
     2641 ggagaacttt ttcttaccgg gtacgttgcc gatgggggtt accattcttg atgaaaatgg
     2701 tcataccctg atttcgctta ccggaccaga aagtaaaatt aagggcgatc ctcgctggat
     2761 gcaggaacgc tcctggtttg gctatacgga agggttccgg gagctggtgc tgaagaaaaa
     2821 tctgccaccc tcatcgctaa gcatcgtgta ttcggtgccg gttgataagg tgctggaacg
     2881 cattcgcatg ttgatcctta acgcaatttt gctgaatgtg cttgccggag ctgcattgtt
     2941 tactctcgca cggatgtacg agcgacgtat tttcattccg gcggaaagcg acgccctgcg
     3001 actggaagaa catgagcagt tcaatcgcaa gattgtcgcc tccgcgccag tgggtatctg
     3061 cattttgcgt accgctgatg gcgtcaatat tttaagtaac gaactggcgc atacctatct
     3121 caatatgctt acgcatgagg accgccaacg actgacgcaa attatctgtg ggcagcaggt
     3181 caattttgtt gatgtcctga ccagcaacaa taccaatctg caaatcagct tcgtccattc
     3241 gcgctatcgt aatgaaaacg tggccatttg tgtgctggtg gatgtttctt cgcgcgtgaa
     3301 gatggaagag tcgttgcagg agatggcaca agcagcggaa caggcgagcc agtcaaaatc
     3361 gatgttcctt gccaccgtca gtcatgagct gcgaacgccg ctgtatggca ttatcggtaa
     3421 cctggatctg ttgcaaacca aagagttacc gaaaggcgtc gatcggctgg tgacggcaat
     3481 gaacaactct tccagcctgt tgttgaaaat tatcagcgat attctcgatt tctcgaagat
     3541 tgaatcggaa cagttgaaga tcgaaccgcg tgagttttca ccgcgtgaag tgatgaacca
     3601 catcaccgcc aactatttac cgctggtggt acgcaagcag ttaggcttgt actgctttat
     3661 tgaaccggat gtgccagtgg ccttaaatgg cgacccgatg cgtttacagc aggtcatctc
     3721 caacctgttg agtaacgcca taaaattcac cgataccggc tgtatagttt tgcatgtccg
     3781 cgctgatggc gattatctct ctatccgtgt tcgcgatacc ggcgtgggga ttccggcgaa
     3841 agaagtggtg cgcttgtttg atcccttctt ccaggtcgga acgggcgtac agcgaaactt
     3901 ccaggggacc ggtctgggtc tggcgatttg tgaaaaactg atcagcatga tggacggcga
     3961 tatctcggta gattcagaac cgggaatggg cagccagttt accgtgcgta ttccgttgta
     4021 cggcgctcag tacccgcaga aaaaaggcgt ggaagggttg agtggtaaac gctgctggct
     4081 ggcggtccgc aatgcgtcgc tctgtcagtt cctggaaacc agtttgcagc gcagcggcat
     4141 cgtcgttaca acatacgaag ggcaggaacc gactcccgaa gatgtgttaa ttactgacga
     4201 ggtagtgagt aaaaaatggc agggtagagc ggtagtgacc ttctgtcgtc gccatattgg
     4261 tattccgctg gagaaagcgc caggggagtg ggtacacagt gtggctgccc cgcatgagct
     4321 accggcattg ttggcgcgta tttatttgat cgagatggag agcgacgatc ctgctaacgc
     4381 tctgccgtcg acggacaaag cggtcagcga taatgacgat atgatgattc tggtcgtgga
     4441 tgatcatccg attaaccgac gtctgctggc agatcagttg ggatcgttgg gctatcaatg
     4501 taaaaccgcg aatgatggtg tcgatgcgct taatgtactt agcaagaatc atattgatat
     4561 cgtgcttagc gacgtcaaca tgccaaatat ggatggttac cgcttgacgc aacgcattcg
     4621 tcagttggga ctgacgttgc cggtaatcgg agtaactgct aatgcgttgg ctgaagagaa
     4681 gcagcggtgt ctggagtccg gtatggacag ctgcctgtcg aagccggtaa cgctggatgt
     4741 gataaaacag acgctgacgg tatatgccga gagggtcagg aaatcgcggg aatcgtagga
     4801 cggataaggt gtttatgccg catctggcat tcagtgcaaa tgccagatgc gacgctgacg
     4861 cgtcttatct ggcctacagg tgattagtct ttatctgccg gacttaacgt cactgaagag
     4921 agataattca gcagggcgat atcgttctcg acacccagct tcatcatcgc agatttcttc
     4981 tggctactga tggttttaat actgcggttc agctttttag cgatctcggt caccaggaag
     5041 ccttccgcaa acaggcgcag aacttcactc tcttttggcg agagacgctt gtcaccgtaa
     5101 ccaccagcac tgattttttc caacaggcga gaaacgcttt ccggggtaaa tttcttccct
     5161 ttctgcagcg cggcgagagc tttcggcaga tcggtcggtg caccttgttt cagcacgatc
     5221 ccttcgatat ccagatccaa taccgcacta agaatcgccg ggttgttgtt catagtcaga
     5281 acaatgatcg acaggcttgg gaaatggcgc ttgatgtact tgattaaggt aatgccatcg
     5341 ccgtacttat cgccaggcat ggagagatcg gtaatcaaca catgcgcatc cagtttcggc
     5401 aggttgttga tcagtgctgt agagtcttca aattcgccga caacattcac ccactcaatt
     5461 tgctcaagtg atttgcgaat accgaacaag actatcggat ggtcatcggc aataattacg
     5521 ttcatattgt tcatgtaata ggctaccttg ctacagcaag ctcttgacat aactgtcaat
     5581 gtcgctgatg tatttttcta ttcctggaac atccttctca cgaatcagat gttccagcgt
     5641 ttcacataac tgcttgccgg gtaccagatt tagcatggca aatacgcctt taagacgatg
     5701 agccgtttgg gctaacgcag caaagtcact ggttgctgct tcagtataca gcctcttaac
     5761 atcatccggt actgtgtcta caaagagcgc ataatagccg ctggcatgga gttgcgcatt
     5821 ttcatctccg cccagaggcg attctgtcac ctcttcctgc gccagttgca cttcaattaa
     5881 ttgtaagacc gcttcctgca tagcgttgct catattgaag ttgacgcaca attgaccagg
     5941 cccaatttcc cgtacgccag actcatcatc gcttaaaagc aagccagagg cagtaagatt
     6001 agacggatta tccgttaaaa agatatcata atcttgacta attaatcttt catcgggtgt
     6061 gatacaggtt gcaccccaat tttctaactg gcgagtgaca atattccgaa tttctgccga
     6121 agtaacatcc accattacgc agacatcatc cagtaaacgc tcttcttcct cttcaacttc
     6181 cgggccagcg gcgagcattt tgatatgcac agagtagcgt gtaccaagcc catcccgcgt
     6241 tttgatgttt aaatgaccgc ccagtttacg tgccagttga tcgctcagcc agaatgccag
     6301 cgggtccgcc tttccataac gatcgttttg ggtctggttg ataaacggga agtgcaaatt
     6361 atccatttca tgaatactta cgccttctcc ggtgtccaga atgcggaatg tcaggcggtc
     6421 ttcagaggac tcatcctgat caacctcaag ggtgattttt cccaattgcg ttgaggtcac
     6481 ggcatattgc atcagtagca gcaaaatacg tcgtaaggca tcgcgatcgc cgcggcgcat
     6541 atcgtgtgct ttcagatgat tgttaatcag cagttgcaga cctttacgct tgatggcagg
     6601 caacactgaa ggcacaactt catcaattaa atcctgcacg gagaacagca ccgtctcact
     6661 tttccagcta tcgtccgcaa gcatgttcgc taactgtatt tcatcgacca gccgcaccag
     6721 cacatctgcc tgattcgcca gttgtttgct ttccggggcg ttgagtttag ccgcgctctc
     6781 cgccagggac tgtgcaggtt ctttcagcgc atcgccaatg tttttcataa aggtcatccg
     6841 gccctgctgg tttttctcat acagacgctg cgcctgcttg agtttcttgt ttaccagcac
     6901 ttcgcgatcc tgatcgcgaa taatgaaaat ttgtgtgcgc ggcgcgacct ggctgcggaa
     6961 catgcggatc tcatacagct cgttattgat cgtcgcctga ataatcccct gatgctgttc
     7021 cgccatggtg gtgatgtttt gcagattcaa atgcggcagc aaatgatcgg caattttgtt
     7081 acttatgaca gtgcggttcg attcctgatc gtgaaccagc aggccgagcg gcagcagtga
     7141 gactatctct tcattgattg cccgtaaaat gcgcaattcg ttattgaccg ccgtgttggg
     7201 tagactttct gtactgcgac tggagaaatg gcggaatgtg gtatagccaa ataacgccag
     7261 cgccagcaaa ccgatgttca gcagcagtgg cagcagaatg ttttgcaacg tatccagcaa
     7321 taaggtgcca taaggaacct gccagaccag gcgcatatcg gtagagttga gtgccgagga
     7381 gatttcaatc ttcgtactgt taaagtggat actgacgcta tccgttcctt ctttctcatt
     7441 atcattgtct cccgttgccg tcgtgtctgg ctcaaggcgg aaactgtcca gcggcatacc
     7501 cggtgggatc aaatcattaa tcggcagatc aaaagccacg accgttgcca gatgtcccgg
     7561 ctggttgaac gtggtacgca aggtaaagta atgaccgttc tgccaggcca ggcggcgcat
     7621 gttagaaaaa ctttcgcgtt catcgagggc gttggcctgt tgcaacattt ctgcacgacg
     7681 tgaatcaaca atgtcactga cggtcgattc tttaaatccg gaggtgagat ctttgagggg
     7741 cagggttgag atcagtacca gactgttatc ctgaccattc aggtaataca tcgaccacgg
     7801 tacattttct gcaccccaca atgtatccag ataagtggac atccgctgag tcatctcaag
     7861 cgttgagttg tcgtgagagc caaagattaa cgcttccgtt ttgcggcgtg gtttctccag
     7921 atagtagaca tcctgtttca ggcgcgtctc ttgtaaacct tcgccggagg agggggaggt
     7981 cgtcgcggca atgttgtcgt agatctgcca ggtcacgtaa cgccaggtat cgacgcgctt
     8041 ttggattgcg tgggtaatgt cgacaatctg gtaactttta tctttcagcc aggcgttaac
     8101 ggcgctttgt accattaccc ccatcgtcac cagtaacaca atgatcaaca gtaaaaagaa
     8161 gcgggtaatg ctccccggta ggagtgaaaa gcgggtcgtg gccgttgtct ctttctgacg
     8221 cattcgtgtt tatgacctgt taaaacttcg cgaagtgata gggcagtata aagggtacag
     8281 tgtgaaattc cagactctta cctgattgat gattctctat tctcccgtga tatttatcat
     8341 cctgcattgg gggaaataga tgtacaagcg ccattttgct gtacaaataa cctacaaaaa
     8401 gcccaacgaa attaattgca aataaacgaa gattgctgga aattatgctg atgttaatta
     8461 tttgtgaaat agttgacaag agttatagtt tttctgtggt agcacagaat aatgaaagtg
     8521 tgtaaagaag ggtaaaaaaa accggatgcg aggcatccgg ttgaaatagg agtaaacaga
     8581 cattcagaaa tgaatgacgg taataaataa agttaatgat gatagcggga gatattctag
     8641 ttgcgagtga aggttttgtt ttgacattca gtgctgtcaa atacttaaga ataagttatt
     8701 gattttaacc ttgaattatt attgct
//
LOCUS       DAZPDU010000169         8696 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZPDU010000169 DAZPDU010000000
VERSION     DAZPDU010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8696
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.171"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..134
                     /gene="yhaV"
                     /locus_tag="ACHURS_003624"
     CDS             <1..134
                     /gene="yhaV"
                     /locus_tag="ACHURS_003624"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFR1239258.1"
                     /translation="NDENTLRTYGKKTNAYTVFSKMLKRGHPPADWETLTRETEETH"
     gene            complement(189..998)
                     /gene="agaR"
                     /locus_tag="ACHURS_003625"
     CDS             complement(189..998)
                     /gene="agaR"
                     /locus_tag="ACHURS_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFR1239259.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            1247..2527
                     /gene="kbaZ"
                     /locus_tag="ACHURS_003626"
     CDS             1247..2527
                     /gene="kbaZ"
                     /locus_tag="ACHURS_003626"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFR1239260.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCNMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGDAHETLSELAVTTPDAARATLEAHRHAFKKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKATALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            2550..3023
                     /gene="agaV"
                     /locus_tag="ACHURS_003627"
     CDS             2550..3023
                     /gene="agaV"
                     /locus_tag="ACHURS_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFR1239261.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            3034..3813
                     /gene="agaW"
                     /locus_tag="ACHURS_003628"
     CDS             3034..3813
                     /gene="agaW"
                     /locus_tag="ACHURS_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFR1239262.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPSAQKEEFEDGI"
     gene            3803..4681
                     /gene="agaE"
                     /locus_tag="ACHURS_003629"
     CDS             3803..4681
                     /gene="agaE"
                     /locus_tag="ACHURS_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFR1239263.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQTDVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            4699..5133
                     /gene="agaF"
                     /locus_tag="ACHURS_003630"
     CDS             4699..5133
                     /gene="agaF"
                     /locus_tag="ACHURS_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFR1239264.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTCLVDELGRCHEECPVEEGI"
     gene            5130..6263
                     /gene="nagA"
                     /locus_tag="ACHURS_003631"
     CDS             5130..6263
                     /gene="nagA"
                     /locus_tag="ACHURS_003631"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFR1239265.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLCITDGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVFDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAAAWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            6614..7768
                     /locus_tag="ACHURS_003632"
     CDS             6614..7768
                     /locus_tag="ACHURS_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFR1239266.1"
                     /translation="MPENYTPAAAATDTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYTPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESTDVIAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            7781..8641
                     /gene="kbaY"
                     /locus_tag="ACHURS_003633"
     CDS             7781..8641
                     /gene="kbaY"
                     /locus_tag="ACHURS_003633"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFR1239267.1"
                     /translation="MSIISTKYLLQDAQANDYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KCFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
BASE COUNT         2005 a         2325 c         2314 g         2052 t
ORIGIN      
        1 tgaacgatga aaacactctg cgcacctacg gtaaaaaaac aaatgcctat accgtattca
       61 gcaaaatgtt aaaaagagga catcctcctg ccgactggga aaccctcacc cgagagacag
      121 aagaaaccca ttgatggtgt tcacaatgcc cgccgaacgc attcgtgttg accagcgggc
      181 accctttctc actccccgac cagaatcact tcaaccccag cctttcgcag tccttccaga
      241 ctatccgcag gaatgccttc atcaacaatg atcatgtcga tacgttgagt atcaatgatc
      301 ttatgtaaac tggagcggtt gaacttactg gaatcggtga ccacgatgat ccgttccgca
      361 acttcgcaca tccggcggtt taaacgggct tcatcttcat tatgtgtgct gacgccgcgc
      421 tccagatcga tcgcatctac accaagaaac agcatatcga agtggtaatt ttgcagcgat
      481 tgctcagcct gatcgccgta aaaagattgc gactgacggc gcaaatgccc gccggtcatc
      541 agcagctcaa cgccttcggc ttccagcaac gcattagcca cgttcatacc gttggtcatc
      601 gcaattacgt cagtgtgctt gcgcatcaga cgagcaatct caaaagtggt ggtcccggaa
      661 tcgaggatca cccgatgacc tggctgaatc aactcaacgg cagctttcgc aacgctgcgt
      721 ttcatcgcgg tgttcagtgc gcttttatct tccactgatg gctcgactga cggcgtcgtg
      781 ctatcgcaga tcaacgcgcc accataggca cgcacagcga tcccctgctt ttccagaaac
      841 gccagatcgt tgcggatcgt cacagtagat acgccataca atgccgacag atcgttaacc
      901 tgcacactcc cttgctgtcg caggcgctga atgatctgtt ctcgtcgctc gctggtgcct
      961 gtcactcgct tctcacctga agcgtcggta ttactcatag taagtccttt cgtaaaactt
     1021 tcgtttcatt tcgttttgcc tattaacgcc tttctattaa gcaaatgcaa gcccaccttg
     1081 cccattgacg caagctactc tcgtttcagt aactttcatt atgtttcttt tgtgaatcag
     1141 atcagaaaac tattatcttt cgttttattt ttatctcacc atggcgcagt atcaactgaa
     1201 acaaaacgaa agattaatat cgcagtaatc tgaactggag aggaaagtga aacatctgac
     1261 agaaatggtg agacagcaca aagcgggcaa aacaaatgga atttatgccg tttgttccgc
     1321 acatccgctg gtgctggagg ctgcaatccg ctacgccagt gcaaaccaaa cgccgttact
     1381 gattgaagca acctccaatc aggtagacca gttcggcggt tataccggaa tgacgcccgc
     1441 cgattttcgc ggctttgttt gtcagctcgc cgactcgttg aatttcccgc aggatgcgtt
     1501 gattctgggt ggtgaccatc tggggccaaa ccgctggcaa aacctgccag ccgctcaggc
     1561 aatggccaat gccgatgatt tgattaaaag ctacgttgcg gcaggattca aaaaaatcca
     1621 ccttgattgc aacatgtcct gtcaggacga tccaattccc ttaactgatg acatcgtggc
     1681 tgaacgcgcc gcccgtctgg cgaaagtggc ggaagaaacc tgtcgcgaac actttggcga
     1741 agccgatctg gagtatgtca ttggtaccga agtgccggta cctggcgacg cgcatgaaac
     1801 cttaagcgag ctggcggtca ccacgccgga tgccgcccgc gccacgctgg aagcccatcg
     1861 tcacgccttt aaaaagcagg gtctgaatgc catctggcca cgcatcattg ccctggtggt
     1921 tcaacccggc gtcgaattcg atcacaccaa cgttattgat tatcagcccg ccaaagcgac
     1981 cgccttaagc cagatggtcg aaaactacga aacgctgata ttcgaagcgc actctacaga
     2041 ttatcaaacg ccgcaatcgc tgcgccagct ggtgattgat cactttgcca ttctgaaagt
     2101 tggcccggcg ctgaccttcg ccctgcgtga agccctgttt tctctggcgg cgattgaaga
     2161 agaactggtg ccagcaaaag cctgttccgg tctgcgtcag gtgctggaaa acgtgatgct
     2221 cgaccggccg gaatactggc aaagccacta ccacggtgac ggcaacgcgc gtcgtctggc
     2281 gcgtggttat agctactcgg atcgcgtgcg ctattactgg ccggacagcc agattgatga
     2341 cgctttcgct catctggttc gtaatctggc ggattcacca attccgctac cgctcatcag
     2401 ccagtatctg ccgctgcaat acgtgaaagt tcgctccggc gagctgcaac cgacgccacg
     2461 ggaactcatt atcaaccata ttcaggacat cctggcgcag taccacacag cctgtgaagg
     2521 ccaataagca aaacaaagag gaacacgcta tgccaaatat tgttttaagc cggattgatg
     2581 aacgcttgat tcacggtcag gtcggcgttc agtgggtcgg atttgcgggg gcaaatctgg
     2641 tgctggtagc caacgatgag gttgccgaag atccggtaca acaaaacctg atggaaatgg
     2701 tactggcaga agggatcgcc gtacgtttct ggacgctgca aaaagttatc gacaacattc
     2761 atcgcgccgc cgatcgacag aaaatcctgc tggtttgtaa aacacccgcc gatttcctga
     2821 cgctggtgaa aggtggcgtt ccggtgaatc gcattaacgt tggcaatatg cactacgcca
     2881 atggcaaaca acaaatcgcc aaaacggttt ctgtggatgc gggcgatatc gcagcattta
     2941 acgacctgaa agccgctggg gtggaatgct tcgttcaggg cgtcccgaca gagcctgctg
     3001 tggacctctt taaattactt tgagggattc atcatggaaa tcagtctgtt gcaggcattc
     3061 gcgttgggca ttatcgcctt tatcgctggc ctggatatgt ttaacggcct aacccatatg
     3121 caccgcccgg tggtcctcgg cccgttggtc gggctggtac ttggcgatct gcataccgga
     3181 attttaaccg gcggtacgct ggaactggtg tggatggggc tggccccgct ggcgggcgca
     3241 cagccgccta acgtgattat cggtactatc gtcggcacgg cgtttgccat tactactggc
     3301 gtgaaacccg atgtcgcagt cggtgtcgcc gtacctttcg ctgtcgcagt acagatgggg
     3361 attaccttcc tgttctcggt gatgtctggc gtgatgtctc gctgcgaccg gatggcggag
     3421 aacgccgata ctcgcggcat tgaacgcgtg aactatctgg cactgctggc actcggcact
     3481 ttctattttc tctgcgcttt cctgcctatc tacttcggcg cggaacatgc aaaaaccatc
     3541 attgatgtcc tgccgcaacg attaatcgac ggcctcggcg tcgcgggcgg catcatgcca
     3601 gcaatcggtt ttgccgtgct gctgaaaatc atgatgaaaa acgtctacat cccctacttc
     3661 atcctgggct ttgttgccgc agcctggctc aagttaccgg tgctggctat tgctgccgct
     3721 gcgctggcga tggcgctgat cgacctgctg cgtaaatctc ctgaaccgac tcaaccttcg
     3781 gcacagaaag aggaatttga agatggcatc taatcaaacc accctgccga acgtcagtga
     3841 aaacgaagaa acattgctga ctggcgtcaa tgaaaacgtg tatgaagatc agagcattgg
     3901 cgcggagctg acgaaaaaag atatcaaccg cgtcgcctgg cgttccatgt tgttacaagc
     3961 gtcttttaac tacgaacgta tgcaggcttc tggctggttg tacggtctgc tgcctgctct
     4021 gaaaaagatc cacactaata aacgcgacct ggcgcgcgcc atgaaggggc atatgggttt
     4081 cttcaatacc catccgtttc tggtgacgtt tgttatcggc attatccttg cgatggagcg
     4141 ttctaagcag gacgttaaca gtattcagag caccaaaatt gccgtcggtg cgccgcttgg
     4201 cgggattggc gatgcaatgt tctggctgac gctactgccg atttgtggcg ggataggcgc
     4261 aagcctcgcg ctacaaggct ctattcttgg cgcagtcgtc tttattgtgc tgttcaacgt
     4321 ggtacacctg gggctgcgtt ttggtctggc gcattatgct taccgcatgg gcgtggcggc
     4381 gattccactc attaaagcga ataccaaaaa agtcggccat gctgcgtcaa tcgttggaat
     4441 gacggtaatc ggcgcactgg tggcaaccta tgttcgttta agcaccacgc tggaaatcac
     4501 agcgggcgac gcagtggtta agttacagac tgatgttatt gacaaactga tgcctgcctt
     4561 cttaccgctg gtctacaccc tgaccatgtt ctggctggta cgccgcggct ggagtccgct
     4621 gcgcctgatt gcagtgaccg tggttctcgg catcgtcggt aaattctgcc atttccttta
     4681 aatacaatga ggtattagat gttaagtatt attttgacag ggcatggcgg gtttgccagt
     4741 ggcatggaaa aagcaatgaa gcaaatcctc ggcgatcaat cgcagtttat tgccatcgat
     4801 tttcccgaaa cctcaagcac cgcgctgctt acatcgcagc ttgaagaggc catcgcccaa
     4861 ctggattgtg aagacggcat tgtttttctg acggatttgc tgggcggcac gccgtttcgc
     4921 gtagcttcaa cgctggcgat gcaaaaaccg ggctgtgaag tgattaccgg caccaatttg
     4981 caactgctac tggagatggt gctggagcgc gaagggttaa gcggtgaaga gttccgtgtg
     5041 caggcgctgg agtgtggaca ccgtgggctg acctgtctgg tggacgagtt aggccgctgc
     5101 catgaggaat gtccggtcga ggaaggaata tgacacacgt tctgcgcgcc agaaggctgc
     5161 tgactgaaga gggatggctc gatgaccatc agttgtgtat tactgacggt gtcatcgcag
     5221 caatcgaacc gattccagtg ggcgtgactg aacgcgatgc ggaactgctc tgccctgctt
     5281 acatcgacac ccatgtacac ggtggtgcgg gcgttgatgt aatggatgac gcgccggatg
     5341 tgttcgacaa gctggcaatg cacaaggcac gcgaaggtgt cggcagttgg ttaccgacca
     5401 ccgtaaccgc gccgcttaat accattcatg cggcgctgaa acgtattgct caacgttgcc
     5461 aacgcggcgg acctggtgcg caagtgctgg ggagttatct cgaaggaccg tacttcacgc
     5521 cgcagaataa aggcgcgcat ccgccggagt tgtttcgcga gcttgaaatt gccgagctgg
     5581 atcagttgat tgccgtttct cagcacacct tacgtgtggt agcgctggca ccggaaaaag
     5641 aaggggcatt gcaggccatc cgccatctta aacagcaaaa cgtaagggtg atgctggggc
     5701 atagcgcggc ggcctggcaa caaacccgcg ccgcatttga tgctggtgcc gacggcctgg
     5761 tgcattgcta taacgggatg acaggtttac atcaccgcga accgggaatg gttggcgcgg
     5821 gattaacgga caagcgcgcc tggctggaac tgatagccga tggtcatcat gtgcatccgg
     5881 cggcaatgtc gctgtgttgt tgctgtgcga aagagagaat cgtactgatc accgacgcga
     5941 tgcaggccgc tgggatgccg gatggtcgct atacgttatg tggtgaagaa gtgcagatgc
     6001 acggtggcgt tgtccgtacc gcgtccggtg ggctggcggg cagtacgcta tctgtcgatg
     6061 cagcagtgcg caacatggtc gagttaacgg gcgtaacgcc tgcggaagcc atccatatgg
     6121 cgtcgctgca tccggcgcga atgctgggtg ttgatggtgt tctgggatcg cttaaaccgg
     6181 gcaaacgcgc cagcgtcgtt gcgctggata gcgggctgca tgtgcaacaa atctggattc
     6241 agggtcaatt agcttcgttt tgatggtttg ctccattatt gggccttcac ttcccccgta
     6301 aggcctttct ttttctttcg ttttgatctg tgcagcggtg ccggatgcga cgctaacgcg
     6361 tctgatccga cctacggtac gccgcatccg gcaatcggtg aacatagcga cgtcattacg
     6421 caattcttta atcttccctt gtcttttctt tcaacgatca cagatttcgt tttatttccc
     6481 ttccctccat tgaactttct gtttcttttc tatagatttt aatcaacgaa agacatcacc
     6541 aagtgaaatg aaacgaaagg caagtgaaag cgacaacgcc cgacgtcaag ttcatcagac
     6601 taaggattga gttatgccag aaaattacac ccctgctgcc gccgcaaccg atacatggac
     6661 tgaagaagag atccgccatc agcctcgcgc atggatccgt tcactcacca acatcgacgc
     6721 gctacgttcc gcgctcaata acttccttga accgttactg cgcaaagaga atctgcggat
     6781 catcctgacc ggagccggaa cgtcggcatt tatcggtgac atcatcgcgc cgtggctcgc
     6841 cagccatacc ggtaaaaact tcagcgccgt accgaccacc gatctggtca ccaatccgat
     6901 ggactacctg aatccagctc atccgctgct gttgatctcc ttcggtcgat ccggcaacag
     6961 cccggaaagc gtcgccgccg tggaactggc aaatcaattt gtacccgagt gctatcacct
     7021 gccgatcacc tgcaacgaag cgggcgctct ttaccaaaac gcgatcaaca gcgataacgc
     7081 gtttgccctg ctgatgcccg cagaaacgca cgatcgcggc tttgcgatga ccagcagcat
     7141 taccaccatg atggccagct gcctcgcggt tttcgcacct gagacgatca acagccaaac
     7201 cttccgcgat gtggcggatc gttgccaggc gatcctgacc tcactgggtg atttcagcga
     7261 aggtgtgttt ggttatacac cgtggaaacg gatcgtttat ctcggcagcg gtggcttaca
     7321 gggcgcagca cgcgagtcgg cgctgaaagt gctggaactg actgcgggta aactggcggc
     7381 cttctatgat tccccaaccg gattccgtca tggcccgaaa tcgctggtcg ataacgaaac
     7441 gctggtggtg gtatttgtct caagccaccc ttacacccgt cagtatgatc ttgatctgct
     7501 ggctgaactt cgccgtgaca accaggcaat gcgcgtaatc gccatcgccg cggaaagcac
     7561 cgacgttatc gccgctggcc cgcatatcat cctgccgcca tcccgtcact ttatcgacgt
     7621 tgagcaggca ttttgcttcc tgatgtacgc ccagacgttt gcactgatgc agtcgctgca
     7681 catgggcaat acgccggata ccccatcagc cagtggcacc gttaaccgcg tggtgcaagg
     7741 cgtaatcatt catccgtggc aggcataaga ggatcgcatt atgagcatta tctccaccaa
     7801 atatctgtta caggacgccc aggccaatga ctacgcggtg cctgccttta acattcataa
     7861 cgccgagacg atccaggcga tcctcgaagt gtgcagtgaa atgcgatcgc cggtgatcct
     7921 cgccggaacg ccggggacct ttaaacatat cgcgctggaa gagatctacg ccctgtgtag
     7981 cgcctattcc acaacctaca acatgccact ggcgctgcat ctcgaccacc acgaatcgct
     8041 ggatgatatt cgccgtaaag tccacgcagg cgtgcgcagt gcgatgatcg acggcagcca
     8101 cttcccgttt gccgagaacg taaagctggt gaaatcggtt gtcgacttct gccactccca
     8161 ggattgcagc gtggaagcag aactgggccg cctgggcggt gttgaagatg acatgagcgt
     8221 tgacgccgaa agtgcattcc tgaccgatcc acaagaagct aaatgctttg tcgaactgac
     8281 tggcgtcgac agcctggcgg tagcgattgg tacggcgcac ggcttataca gcaaaacgcc
     8341 gaagattgat ttccagcggc tggcggaaat tcgtgaagtg gtggatgttc ctctggtgct
     8401 gcatggtgcc agcgatgttc cggatgaatt tgtccgtcgc actattgaac ttggcgtcac
     8461 aaaagtgaac gttgccacag aattaaaaat agccttcgct ggcgcggtta aagcctggtt
     8521 tgcggaaaat ccgcagggta atgatcctcg ttattatatg cgcgtcggaa tggatgcgat
     8581 gaaagaagtt gtcagaaata aaattaatgt ctgtggttca gcgaatcgaa tttcagcata
     8641 atcattagtt ttttatgatt tatcccaatg tacttcccga ttaatatcgg ggagtg
//
LOCUS       DAZPDU010000170         8651 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZPDU010000170 DAZPDU010000000
VERSION     DAZPDU010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8651
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.172"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            94..1737
                     /gene="eptA"
                     /locus_tag="ACHURS_003634"
     CDS             94..1737
                     /gene="eptA"
                     /locus_tag="ACHURS_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFR1239268.1"
                     /translation="MLKRLLKSPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQQAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDVLQTCRRVSE"
     gene            1734..2402
                     /gene="basR"
                     /locus_tag="ACHURS_003635"
     CDS             1734..2402
                     /gene="basR"
                     /locus_tag="ACHURS_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFR1239269.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYTCDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            2403..3503
                     /gene="pmrB"
                     /locus_tag="ACHURS_003636"
     CDS             2403..3503
                     /gene="pmrB"
                     /locus_tag="ACHURS_003636"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFR1239270.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQYVANQI"
     gene            complement(3479..3568)
                     /gene="pmrR"
                     /locus_tag="ACHURS_003637"
     CDS             complement(3479..3568)
                     /gene="pmrR"
                     /locus_tag="ACHURS_003637"
                     /inference="COORDINATES: protein motif:HMM:NF033224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFR1239271.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(3680..5182)
                     /gene="proP"
                     /locus_tag="ACHURS_003638"
     CDS             complement(3680..5182)
                     /gene="proP"
                     /locus_tag="ACHURS_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFR1239272.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(5446..6312)
                     /locus_tag="ACHURS_003639"
     CDS             complement(5446..6312)
                     /locus_tag="ACHURS_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFR1239273.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSGGEHADFLSWLGKWSTADKQPVCWSVTQRWQTVALGMPRLCSAQRLVG
                     AMVEEIFSVNLA"
     gene            complement(6309..8537)
                     /gene="crfC"
                     /locus_tag="ACHURS_003640"
     CDS             complement(6309..8537)
                     /gene="crfC"
                     /locus_tag="ACHURS_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFR1239274.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELSKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTCCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHVNTRQLFSEVISRQDATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFCAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2204 a         2302 c         2129 g         2016 t
ORIGIN      
        1 tccagcaaac ataaaagcca accttaagaa cttaaggttg gcttaatttt gctttgcgag
       61 catatgcgca ctttgttcga tggaaacacc gtgatgttga agcgcctact aaaaagcccc
      121 tctttgaatt tactcgcctg gctattgttg gccgcttttt atatctctat ctgcctgaat
      181 attgcctttt ttaaacaggt gttgcaggcg ctgccgctgg attcgctgca taacgtactg
      241 gttttcttgt cgatgccggt cgtcgctttc agcgtgatta atattgtcct gacactaagc
      301 tctttcttat ggcttaatcg accactggcc tgcctgttta ttctggttgg cgcggctgca
      361 caatatttca taatgactta cggcatcgtc atcgaccgct cgatgattgc caatattatt
      421 gataccactc cagcagaaag ttatgcgctg atgacaccgc aaatgttatt aacgctggga
      481 ttcagcggcg tgcttgctgc gctgattgcc tgctggctaa aaatcaaacc tgccacctcg
      541 cgtctgcgca gtgttctttt ccgtggagcc aatattctgg tttctgtact gctgattttg
      601 ctggtcgccg cactgtttta taaagactac gcctcgttgt tccgcaataa caaagagctg
      661 gtgaaatcct taagcccctc taacagcatt gttgccagct ggtcatggta ctcccatcag
      721 cgactggcaa atctaccgct ggtgcgaatt ggtgaagacg cccaccgcaa cccgttaatg
      781 cagaacgaaa aacgtaaaaa tttgaccatc ctgattgtcg gcgaaacctc gcgggcggag
      841 aacttctccc tcaacggcta cccgcgtgaa actaacccgc ggctggcgaa agataacgtg
      901 gtctatttcc ctaataccgc atcttgcggc acggcaacgg cagtttcagt accgtgcatg
      961 ttctcggata tgccgcgtga gcattacaaa gaagagctgg cacagcacca ggaaggcgtg
     1021 ctggatatca ttcagcaagc gggcatcaac gtgctgtgga atgacaacga tggcggctgt
     1081 aaaggtgcct gcgaccgcgt gcctcaccag aacgtcaccg cgctgaatct gcctggtcag
     1141 tgcatcaacg gcgaatgcta tgacgaagtg ctgttccacg ggctggaaga gtacatcaat
     1201 aacctgcagg gtgatggcgt gattgtctta cacaccatcg gcagccacgg tccgacctat
     1261 tacaaccgct atccgccgca gttcaggaaa tttaccccaa cctgcgacac caatgagatc
     1321 cagacctgta ccaaagagca actggtgaac acttacgaca acacgctggt ttacgtcgac
     1381 tatattgttg ataaagcgat taatctgctg aaagaacatc aggataaatt taccaccagc
     1441 ctggtttatc tttctgacca cggtgaatcg ttaggtgaaa atggcatcta tctgcacggt
     1501 ctgccttatg ccatcgcccc ggatagccaa aaacaggtgc cgatgctgct gtggctgtcg
     1561 gaggattatc aaaaacggta tcaggttgac cagaactgcc tgcaaaaaca ggcgcaaacg
     1621 caacactatt cacaagacaa tttattctcc acgctattgg gattaactgg cgttgagacg
     1681 aagtattacc aggctgcgga tgatgttctg caaacttgca ggagagtgag tgaatgaaaa
     1741 ttctgattgt tgaagacgat acgctgttat tgcagggact gattctggcg gcgcaaaccg
     1801 aaggctacac gtgcgatggc gtaacaaccg cgcggatggc ggaacaaagc cttgaggccg
     1861 gtcattacag cctggtggta ctggatttag ggttacccga cgaagatgga ctgcattttc
     1921 tcgcccgtat ccggcagaaa aaatacaccc tgccggtact gatcctcacc gctcgcgata
     1981 cgctgaccga caaaatcgcc gggctggatg tcggtgccga tgactatctg gtgaagcctt
     2041 ttgcgctgga agagttacac gcccgtatcc gcgccctgct acgacgccat aataatcagg
     2101 gcgaaagtga gctgattgtt ggcaatctga cgctgaatat gggtcgccgt caggtatgga
     2161 tgggcggtga agagttgatt ctgacgccca aagaatatgc tctgctgtca cggttaatgc
     2221 tcaaagcagg cagtccggta catcgggaaa ttctctacaa cgacatctat aactgggaca
     2281 atgaaccctc gaccaacacc ctggaagtgc atatccacaa tctgcgcgac aaagtgggca
     2341 aagcccgtat ccgcaccgtg cgcggctttg gctatatgct ggtcgcgaat gaggaaaact
     2401 aattgaatct gatgcgtttt ctgcgccgac caatatcgct gcgccaacgg ctgatattga
     2461 ccatcggggc cattttgttg gtgtttgagc tgatcagcgt cttctggcta tggcatgaaa
     2521 gtaccgagca gattcagctg tttgagcagg cgctacgcga caatcgcaac aacgatcgcc
     2581 acatcatgcg tgagatccgc gaggcggtcg ccagcctgat tgtccccggc gtctttatgg
     2641 tcagcctgac gctatttatc tgctatcagg cggtacgccg catcacccgc ccgctggcgg
     2701 agctgcaaaa agagctggaa gcgcgcaccg ccgacaactt aacgcccatt gccattcaca
     2761 gcgccaccct cgaaatcgaa gcggtggttt cggcgttgaa cgatctggtc agtcgcctga
     2821 cgagcacgct ggataacgaa aggttattta ccgctgacgt cgcgcacgaa ctgcgaacgc
     2881 cactggcggg ggtgcgtttg catctggaac tgctggcgaa aacgcatcac attgatgtag
     2941 caccgttagt ggcacggctt gatcagatga tggagagcgt ctcccagctg ctgcaactgg
     3001 cgcgtgccgg acagtcattt tcttccggta attatcaaca tgtaaaactg ctggaagatg
     3061 tcattctccc ctcgtatgac gaactcagta ccatgctcga ccagcgacag caaaccctgc
     3121 tattgccaga gagcgccgcc gacatcaccg ttcagggtga tgcaaccctg ctgcggatgt
     3181 tattgcgaaa cctggtagaa aacgcccatc gttacagccc acaaggcagc aacattatga
     3241 ttaagctgca agaagatggc ggagcggtca tggccgttga agatgaagga ccgggtattg
     3301 atgagagtaa atgcggggag ttgagtaaag cgtttgtacg tatggacagc cgttatggcg
     3361 ggattggtct ggggttaagt attgtcagcc gcattacaca gttgcatcac gggcagtttt
     3421 tcctgcaaaa ccggcaagag acttccggca cgcgggcctg ggtacggctg aagaaagatc
     3481 agtacgtagc aaaccagata taaagaaagc tgctgaccac cagcacactg aacacggtag
     3541 ttaaactttc ataaacacgg tttttcattg cactctcctc cgttaacctg gaggagagta
     3601 tgcgcgtcag agattaagtg aaccttaaga gttcaattgg cctgataaga cagcgtcaca
     3661 tcaggccatc cgtttcagct tattcatcaa ttcgcggatg ttgctgcacc aggcgggtac
     3721 gtttcgcctg caaatcggca atctcgtggt caatatcatc gattttctgc tcgatattat
     3781 cgtaatgctc gacgagaatt tccttcgctt cctgtatatc tgacgccgcc ggtgtcgcac
     3841 ctttcaacgg acgatttgcc gtctctttca tggttacgcc ggtgattaaa ccaatcaccg
     3901 ccactaccat caggtaatag gcaggcatca tcagattctg cgagctttcg accagccagg
     3961 ccgccagtgt tggcgtcaga ccggcaacca gcaccgaaat attaaatgcc gccgccagcg
     4021 cgctgtaacg gatatgcgtc gggaacatcg ctggcaaggt agaagccata acgcccgtaa
     4081 agcagttaag gatcaccgcc agcatcagta acccggcaaa aatcaggccg atgacgttac
     4141 tgttaatcag aataaacgcc gggatcgcca acacaaacag ggcaacacta ccaagtagca
     4201 caaacggacg acggccaaaa cggtcactca gcaagcccat caccggctgg acaaacagca
     4261 taccgatcat aatggcgata ataatcagca ccccgtggtc ttcggagtaa tgcaggttat
     4321 gcgacaaata actcggcata taggtcagca acatgtagta agtcacgttg gtggcaatta
     4381 ccagaccaat acatgtcaac aggctgcgcc agtatttagt ggcaatctct ttaaacgaga
     4441 ctttcgggcc atcctgcaaa ccttcgcggt cgccctgttc cagtttatca acatgctgct
     4501 ggaacgccgg agtttcttcc agcgcatggc gcaggtaaag cccgataatc cctaacggca
     4561 gagcaataaa gaacggaata cgccagcccc agtcgaggaa gttcgcttcg ccgacaatgg
     4621 tcgaaattaa caccaccacg cccgcaccca gcacaaaccc ggcaatagaa ccgaagtcca
     4681 gccagctgcc cataaagcca cgtttacggt caggggagta ttccgcaaca aatatcgacg
     4741 ccccggtata ttcaccgccg accgagaaac cttgtgccat cttacagatc aacagcagaa
     4801 tcggtgccca aataccaatc gtgtcgtagg acggtattaa gccaatacag aacgtactga
     4861 tcgacataat cacaatagtg atagcgagga tcttctggcg accatattta tcgcccaaca
     4921 taccaaagaa gagcccgcca agcggtcgaa tcagaaaggg aacggagaaa gtggcaagtg
     4981 cagcaaccat ctgcacgctg gggtcagccc ccgggaaaaa aactttacct aatgcgtaag
     5041 caacaaaacc ataaacacca aaatcgaacc attccattgc attacccagt gatgctgcgg
     5101 taatggcttt acgcagttta ccgtcatcaa taatggtgac atcacgaagg gtaatcggtt
     5161 ttactttttt ccttttcagc atagctttcc tcgcagagtt ggcccatttc cgcaaacgcc
     5221 agaaaaatca gcgcgacggc cgcaaaaggg gagcgggaat tgcaggatgc aatctctgta
     5281 acaagcgtag cgggtttgct tacaccctcc ggttaaggaa tgtacaatca aaccagttga
     5341 cgcctgaatt gggcagttaa tgaccttttt accctaccag tgtttaaatt tgtgatcaac
     5401 ttcacacatg gatttcgttt aggactcatt gatgtaactg attatttacg ccaaatttac
     5461 agagaagatt tcctcgacca ttgcccccac caaacgctgc gctgaacaga gtcgtggcat
     5521 ccccagcgcg actgtttgcc agcgttgggt cactgaccag caaacgggct gtttatctgc
     5581 cgtggaccac tttcccagcc aacttaaaaa atcagcatgc tcccctcccg aagaggcaaa
     5641 ccactgatgg taatagtgac gcgtggtcgg tgccgcattg atactctctg ccagatgttg
     5701 tgtcgctttc tcgcgcaaat cacccagtaa caacaccacc gcttgcgggc ttcccagacg
     5761 gcaggcatcg ccaaatgcac cccagcgcaa ctcctccaga acgataaaac agcgcccagg
     5821 caacgaccag gcgttatacg ccccagcacg ccagcgggta aaaatttgct cgctctgatc
     5881 acctgcctgc accagtaggc ctcgttgcgt taacgccttt tccttccagc gacaacgggc
     5941 tgaaaaccac tcatacaagg cggcaatttg ctgggggagc tccgggaaag cagagtgctg
     6001 gcggtgatcc attttttgca gcagcgccag atgctgcgcg atctgcgtca gcgccagatg
     6061 accgggatcc agcatcgacg ccagtttttc caccattgtc agtcgcaggc taagcggcgc
     6121 atttaacatg ccgccgttcg tccatgtctg aagctgcaca cgcgaaagaa gttcctgcat
     6181 cagtcgttca cgaaactgct gctgatgggc ttgtggaagg cgggcatcat cgccctgcgc
     6241 cagatccact aaaaagcggg gataaaccga ctccagcacg cgaccggggc cgtccagtaa
     6301 cgtcttggtc aatatcgttc tgccgtgaaa agtgtttgaa tatcatcgcg taacagctgg
     6361 gcgtcggtgt aaatccagcc gtgagtcatc acagtctgct gcaattgctg ctgcatcagc
     6421 ctgaccaccg attcattttg ttgacgcaga gccaggcttt cgcgtaaacg cgtctgtaat
     6481 tccgtcaaac atgaagcgaa ctcagcgcaa aaagtattca tgcctgccgt aacagattca
     6541 tcgacctgct ctgccagaac tttagccatt tgttggcaat aaagatcgac ttctgcgctt
     6601 aaagctcgtt gcaacacact gtaatcaacc gtctctgtcg gggatttctc atttccccgt
     6661 ccccagtcgg gctgattcaa ccagcgcgaa aaagtctcac gcacaacgcc taaacgcgtg
     6721 ctctgctcgt ccgttgcatc ctggcgcgaa atgacttcac tgaacagctg gcgagtgttg
     6781 acgtggggaa ctactccgtg aaaaacagga aaatgaaacc caggacgaaa ccctgactcg
     6841 ctcaattcca ttttgacttg ttgctcaatg ggccgaataa catcggttaa cacccggcaa
     6901 agggtggatt caagctcggc aaaacgcagc gtaaagtcgc gactgatggt gttctgcgcc
     6961 gtctgtaaca gtgtctcaca gcaggtacga atctcgctta acggctccga atcatcctga
     7021 aacaaggcgg ctaactgcgc attcagcgca tcttgctgtt gacgcagaaa gtggttggcg
     7081 gaggtcaggg ccagctcgat ttcatgttta atctcgccgc tcacctgcgc ctgattgagt
     7141 tgcaatagct gcaaactttc ttcgacctga tggatatttt gccgcaattg ttcacaagcg
     7201 acgtttaacc cgtgcgcacg aaaatccagg tattcccgcg cctgctgcgc gtaattcaac
     7261 agtttatgcg cagcagatcg cagagcatac aacgaggcgt tagcgtaagc ggcatgaagc
     7321 aacgcctgaa ttggctgggc gaacagcgaa tcttcccaca actgatcggc agcatgacga
     7381 atatgttcga ggtccgccag atcggcatga cgccagcgcc tgccgagcgc ggcatgggca
     7441 aaatcttcca cccagcgttg ttgctctggc gctggtaact taccgttgtt ggctaactca
     7501 tggcgcgctc gatttgccaa gtagccccac atcgacgaca ccggaaatat ctgctgtggc
     7561 gtaatacagc ctttcatcag cgtcccggaa atcagtgccc gcacctggtc ggcgtcgtca
     7621 ctgttacgat cctgttgatc gaacttattg accagcacat acagtggcac cgattgcccc
     7681 accgccaaaa tcgcctcacg gacctcttca tcggagatcg atttcagttg cgtataatcc
     7741 agcaccgcca gtaccgccga ggcgcgtgcc agctgctggt taagcatttt ttgcagatgc
     7801 ggttgcccgg cttcatttgg cccgggggta tccagtaacg tcaactgacc gggataactc
     7861 tccagccccg ccagatggac aaactccact tcaatcacgg gaatatgctc aatggcggcg
     7921 taagcagaaa aaggaaaatc gacgtccagc gccttcgcca gtcgcactaa atcattcaaa
     7981 cttttcagac aatgaaaaat aggctgggca cccagataat atttttcgaa agcgacgcca
     8041 ttttcgatcc gctgcataag cgcacgcata tctttatcta tttccagcac atcggtcaga
     8101 tgcttaatat cgcaatcacg caggcgctgt tgtaattgtt gaattaaaca atcgattggc
     8161 gcgacatgtg aaaaatgcag taccggttcc ttttgcccgg gcgtatggcg aataagcgtc
     8221 ggcagcgcag tcattgggcg attacgatta ggcagaacct ccgtaccaac aatggcatta
     8281 atggtggttg atttccctgc tttcatagta ccgacaatcg ccagcaccat ttccagtcgg
     8341 gaaattttac tcaactcatt attcagcatc gcgtgacgtt cggcgatatt aggctgactc
     8401 cagggtaaag ccagttgtgg cgcgtcgtct ccgggtacag agagaggcat tttttccagt
     8461 aactgcaact gttggcgaga aagctgtaac aggcgttcag cctcctgact taactcatac
     8521 agggtctgtg tgtacataga aaattcttcc ttaaagcaaa ttttgttact tttatttagc
     8581 cagattgttt ttgagttctg tttttggctt ttataattac tgcaagaaat aattttatat
     8641 ttagtgtgtt g
//
LOCUS       DAZPDU010000171         8636 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZPDU010000171 DAZPDU010000000
VERSION     DAZPDU010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8636
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.173"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(260..523)
                     /gene="rpsT"
                     /locus_tag="ACHURS_003641"
     CDS             complement(260..523)
                     /gene="rpsT"
                     /locus_tag="ACHURS_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFR1239275.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            626..844
                     /locus_tag="ACHURS_003642"
     CDS             626..844
                     /locus_tag="ACHURS_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFR1239276.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            852..1793
                     /gene="ribF"
                     /locus_tag="ACHURS_003643"
     CDS             852..1793
                     /gene="ribF"
                     /locus_tag="ACHURS_003643"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFR1239277.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1836..4652
                     /gene="ileS"
                     /locus_tag="ACHURS_003644"
     CDS             1836..4652
                     /gene="ileS"
                     /locus_tag="ACHURS_003644"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFR1239278.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4652..5146
                     /gene="lspA"
                     /locus_tag="ACHURS_003645"
     CDS             4652..5146
                     /gene="lspA"
                     /locus_tag="ACHURS_003645"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFR1239279.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSR
                     AKKQ"
     gene            5271..5720
                     /gene="fkpB"
                     /locus_tag="ACHURS_003646"
     CDS             5271..5720
                     /gene="fkpB"
                     /locus_tag="ACHURS_003646"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR1239280.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5722..6672
                     /gene="ispH"
                     /locus_tag="ACHURS_003647"
                     /gene_synonym="lytB"
     CDS             5722..6672
                     /gene="ispH"
                     /locus_tag="ACHURS_003647"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFR1239281.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6738..7652
                     /gene="rihC"
                     /locus_tag="ACHURS_003648"
     CDS             6738..7652
                     /gene="rihC"
                     /locus_tag="ACHURS_003648"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFR1239282.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPIGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSSGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            7819..>8636
                     /gene="dapB"
                     /locus_tag="ACHURS_003649"
     CDS             7819..>8636
                     /gene="dapB"
                     /locus_tag="ACHURS_003649"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFR1239283.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKENGLFDMRDVLNLNN"
BASE COUNT         1964 a         2159 c         2459 g         2054 t
ORIGIN      
        1 atgtgtatca caatatccac attctttgta ggggagtctg gagattgagt agatattctt
       61 gttcagaatg tatcagccga tggttctacg attcttaagc cacgaagagt tcagaaagtg
      121 taacggcatg tctgcaaaag ccagcatctt ttgactatct ggcaaccggt agtgtgttct
      181 ctcacgcatc ataaaagcag caggcataaa aaaaacccgc ttgcgcgggc tttttcacaa
      241 agcttcagca aattggcgat taagccagtt tgttgatctg tgcagtcagg ttagccttat
      301 gacgtgcagc tttgtttttg tggatcagac ctttagcagc ctgacggtcc acgatcggtt
      361 gcatttcgtt aaatgctttc tgtgcagcag ctttgtcgcc agcttcgata gctgcgtata
      421 ctttcttgat gaaagtacgc atcatagagc gacggcttgc gttgtgctta cgagcctttt
      481 cagactgaat ggcgcgcttc ttagctgatt tgatattagc caaggtccaa ctcccaaatg
      541 tgttctatat ggacaattca aaggccgagg aatatgccct tttagccttc ttttgtcaat
      601 ggatttgtgc aaataagcgc cgttaatgtg ccggcactcg ttacgtagtg atggcgcagg
      661 attctaccag cttgcggggt gtgaatacag cttttccgcg ataaaaattg cagcaggcgg
      721 tcactttctt cccgtgattt gcgccatggc aatgaaaagc cacttctttc tgatttcggt
      781 actcaatcgc cggttaacct tgaccgctgt acaaggtata ctcggacgat tttcactgtt
      841 ttgagccaga catgaagctg atacgcggca tacataatct cagccaggcc ccgcaagaag
      901 ggtgtgtgct gactattggt aatttcgacg gcgtgcatcg cggtcatcgc gcgctgttac
      961 agggcttgca ggaagaaggg cgcaagcgca acttaccggt gatggtgatg ctttttgaac
     1021 ctcaaccact ggaactgttt gctaccgata aagccccggc aagactgacc cggctgcggg
     1081 aaaaactgcg ttaccttgca gagtgtggcg ttgattacgt gctgtgcgtg cgtttcgaca
     1141 ggcgtttcgc ggcgttaacc gcgcaaaatt tcatcagcga tcttctggtg aagcatttgc
     1201 gcgtaaaatt tcttgccgta ggggatgatt tccgctttgg cgctggtcgt gaaggcgatt
     1261 tcttgttatt acagaaagct ggcatggaat acggcttcga tatcactagt acgcaaactt
     1321 tttgcgaagg tggcgtgcgt attagcagca ccgctgtgcg tcaggcgctg gcggatgaca
     1381 atctggctct ggcagaaagt ttactggggc acccgtttgc catctccggg cgtgtagtcc
     1441 acggtgatga attagggcgc actataggtt tcccgacggc gaatgtaccg ctacgccgtc
     1501 aggtttcccc ggtgaaaggg gtttatgcgg tagaagtgct gggcctcggt gaaaagccgt
     1561 tacccggcgt ggcaaacatc ggaacacgcc caacggttgc cggtattcgc cagcagctgg
     1621 aagtgcattt gttagatgtt gcaatggacc tttacggtcg ccatatacaa gtagtgctgc
     1681 gtaaaaaaat acgcaatgag cagcgatttg cgtcgctgga cgaactgaaa gcgcagattg
     1741 cgcgtgatga attaaccgcc cgcgaatttt ttgggctaac aaaaccggct taagcctgtt
     1801 atgtaatcaa accgaaacac ggaaccgaga atctgatgag tgactataaa tcaaccctga
     1861 atttgccgga aacagggttc ccgatgcgtg gcgatctcgc caagcgcgaa ccgggaatgc
     1921 tggcgcgttg gactgatgat gatctgtacg gcatcatccg tgcggctaaa aaaggcaaaa
     1981 aaaccttcat tctgcatgat ggccctcctt atgcgaatgg cagcattcat attggtcact
     2041 cggttaacaa gattctgaaa gacattatcg tgaagtccaa agggctttcc ggttatgact
     2101 cgccgtatgt gcctggctgg gactgccacg gtctgccgat cgagctgaaa gtagagcaag
     2161 aatacggtaa gccgggtgag aaattcaccg ccgccgagtt ccgcgccaag tgccgcgaat
     2221 acgcggcgac ccaggttgac ggtcaacgca aagactttat ccgtctgggc gtgctgggcg
     2281 actggtcgca cccgtacctg accatggact tcaaaactga agccaacatc atccgcgcgc
     2341 tgggcaaaat catcggcaac ggtcacctgc acaaaggcgc gaagccagtt cactggtgcg
     2401 ttgactgccg ttctgcgctg gcggaagcgg aagttgagta ttacgacaaa acttctccgt
     2461 ccatcgacgt tgcttttcag gcggtcgatc aggatgcact gaaagcaaaa tttgccgtaa
     2521 gcaacgttaa cggcccaatc tcgctggtga tctggaccac tacgccgtgg actctgcctg
     2581 cgaaccgcgc aatctctatt gcacctgatt tcgactatgc gctggtgcag atcgacggtc
     2641 aggccgtgat tctggcgaaa gatctggttg aaagcgtaat gcagcgtatc ggcgtgaccg
     2701 attacaccat tctcggcacg gtaaaaggtg cggagcttga gttgctgcgc tttacccatc
     2761 cgtttatggg cttcgacgtt ccggcaatcc tcggcgatca cgttacccta gatgccggta
     2821 ccggtgccgt tcacaccgcg cctggccacg gcccggacga ctatgtgatc ggtcagaaat
     2881 acggcctgga aaccgctaac ccggttggcc cggacggcac ttatctgccg ggcacttatc
     2941 cgacgctgga tggcgtgaac gtcttcaaag cgaacgacat cgtcgttgcg ctgctgcagg
     3001 aaaaaggcgc gctgctgcac gttgagaaaa tgcagcacag ctatccgtgc tgctggcgtc
     3061 acaaaacgcc gatcatcttc cgcgcgacgc cgcagtggtt cgtcagcatg gatcagaaag
     3121 gtctgcgtgc gcagtcactg aaagagatca aaggcgtgca gtggatcccg gactggggcc
     3181 aggcgcgtat cgagtcgatg gttgctaacc gtcctgactg gtgtatctcc cgtcagcgca
     3241 cctggggcgt accgatgtca ctgttcgtgc acaaagacac ggaagagctg catccgcgta
     3301 ctctcgaact aatggaagaa gtggcaaaac gcgttgaagt tgacggcatc caggcgtggt
     3361 gggatcttga tgcgaaagag atcctcggcg atgaagctga tcagtacgtg aaagtgccgg
     3421 acacattgga tgtatggttt gactccggat ctacccactc ttctgttgtt gacgtgcgtc
     3481 cggaatttgc cggtcacgca gcggacatgt atctggaagg ttctgaccag caccgtggtt
     3541 ggttcatgtc ttccctaatg atctccaccg cgatgaaagg caaagcgccg tatcgtcagg
     3601 tactgaccca cggctttacc gtggatggtc agggtcgcaa gatgtctaaa tccatcggca
     3661 ataccgtttc gccgcaggat gtgatgaaca aactgggcgc ggatattctg cgtctgtggg
     3721 tggcatcaac cgactacacc ggtgaaatgg ccgtttctga cgagatcctg aaacgtgctg
     3781 ccgatagcta tcgtcgtatc cgtaacaccg cgcgcttcct gctggcaaac ctgaacggtt
     3841 ttgatccagc aaaagatatg gtgaaaccgg aagagatggt ggtactggat cgctgggccg
     3901 taggttgtgc gaaagcggca caggaagaca tcctcaaggc gtacgaagca tacgatttcc
     3961 acgaagtggt acagcgtctg atgcgcttct gctccgttga gatgggttcc ttctacctcg
     4021 acatcatcaa agaccgtcag tacaccgcca aagcggacag tgtggcgcgt cgtagctgcc
     4081 agactgcgct gtatcacatc gcagaagcgc tggtgcgctg gatggcacca atcctctcct
     4141 tcaccgctga tgaagtgtgg ggctacctgc cgggcgaacg tgaaaaatac gtcttcaccg
     4201 gtgagtggta cgaaggcctg tttggcctgg cagacagtga agcgatgaac gatgcgttct
     4261 gggacgagct gttgaaagtg cgtggcgaag tgaacaaagt cattgagcaa gcgcgtgccg
     4321 acaagaaagt gggtggctcg ctggaagcgg cagtaacctt gtatgcagaa ccggaactgt
     4381 cggcgaaact gaccgcgctg ggcgatgaat tacgatttgt cctgttgacc tccggcgcta
     4441 ccgttgcaga ctataacgac gcacctgctg atgctcagca gagcgaagta ctcaaagggc
     4501 tgaaagtcgc gttgagtaaa gccgaaggtg agaagtgccc acgctgctgg cactacaccc
     4561 aggatgtcgg caaggtggcg gaacacgcag aaatctgcgg ccgctgtgtc agcaacgtcg
     4621 ccggtgacgg tgaaaaacgt aagtttgcct gatgagtcaa tcgatctgtt caacagggct
     4681 acgctggctg tggctggtgg tagtcgtgct gattatcgat ctgggcagca aatacctgat
     4741 cctccagaac tttgctctgg gggatacggt cccgctgttc ccgtcgctta atctgcatta
     4801 tgcgcgtaac tatggcgcgg cgtttagttt ccttgccgat agcggcggct ggcagcgttg
     4861 gttctttgcc ggtattgcga ttggtattag cgtgatcctg gcagtgatga tgtatcgctc
     4921 gaaggccacg cagaagctaa acaatatcgc ttacgcgctg attattggcg gcgcgctggg
     4981 caacctgttc gaccgcctgt ggcacggctt cgttgtcgat atgatcgact tctacgtcgg
     5041 cgactggcac ttcgccacct tcaaccttgc cgatactgcc atctgtgtcg gtgcggcact
     5101 gattgtgctg gaaggttttt tgccttctag agcgaaaaaa caataataaa ccctgccgga
     5161 tgcgatgctg acgcatctta tccggcctac agattgctgc gaaatcgtag gccggataag
     5221 gcgtttacgc cgcatccggc aaaaatcctc aaatataaga gcaaacctgc atgtctgaat
     5281 ctgtacagag caatagcgcc gtcctggtgc acttcacgct aaaactcgac gatggcacca
     5341 ccgccgagtc tacccgcaac aacggtaaac cggcgctgtt ccgcctgggt gatgcttctc
     5401 tttctgaagg gctggagcaa cacctgttgg ggctgaaagt gggcgataaa accaccttct
     5461 cgttggagcc agatgcggcg tttggcgtgc cgtcaccgga cctgattcag tacttctccc
     5521 gccgtgaatt tatggatgca ggcgagccag aaattggcgc aatcatgctt tttaccgcaa
     5581 tggatggcag tgagatgcct ggcgtgatcc gcgaaattaa cggcgactcc attaccgttg
     5641 atttcaacca tccgctggcc gggcagaccg ttcattttga tattgaagtg ctggaaatcg
     5701 atccggcatt ggaggcgtaa catgcagatc ctgttggcca acccgcgtgg tttttgtgcc
     5761 ggggtagacc gcgctatcag cattgttgaa aacgcgctgg ccatttacgg cgcaccgata
     5821 tatgtccgtc acgaagtggt acataaccgc tatgtggtcg atagcttgcg tgagcgtggg
     5881 gctatcttta ttgagcagat tagcgaagta ccggacggcg cgatcctgat tttctccgca
     5941 cacggtgttt ctcaggcggt acgtaacgaa gcaaaaagcc gcgatttgac ggtgtttgat
     6001 gccacctgtc cgctggtgac caaagtgcat atggaagtcg cccgcgccag ccgtcgtggc
     6061 gaagagtcga ttctcatcgg ccacgccggg catccggaag tggaaggtac aatgggccag
     6121 tacagtaacc cggaaggggg aatgtatctg gtcgaatcgc cggacgatgt gtggaaactg
     6181 acggtcaaaa acgaagagaa gctctccttt atgacccaga ccacgctgtc ggtggatgac
     6241 acgtctgatg tgatcgacgc gctgcgtaaa cgcttcccga aaattgtcgg tccgcgcaaa
     6301 gatgacatct gctacgccac gactaaccgt caggaagcgg tacgcgccct ggcagaacag
     6361 gcggaagttg tgttggtggt cggttcgaaa aactcctcca actccaaccg tctggcggag
     6421 ctggcccagc gtatgggcaa acgcgcgttt ttgattgacg atgcgaaaga tatccaggaa
     6481 gagtgggtga aagaggttaa atgcgtcggc gtgactgcgg gcgcatcggc tccggatatt
     6541 ctggtgcaaa atgtggtggc acgtttgcag cagctgggcg gtggtgaagc cattccgctg
     6601 gaaggccgtg aagaaaacat tgttttcgaa gtgccgaaag agctgcgtgt cgatattcgt
     6661 gaagtcgatt aagtcattag cagcctaagt tatgcgaaaa tgccggtctt gttaccggca
     6721 ttttttatgg agaaaacatg cgtttaccta tcttcctcga tactgacccc ggcattgacg
     6781 atgccgtcgc cattgccgcc gcgatttttg cccccgaact cgacctgcaa ctgatgacca
     6841 ccgtcgcggg taatgtctcg gttgagaaaa ccacccgcaa cgccctgcaa ctgctgcatt
     6901 tctggaatgc ggagattccg ctcgcccagg gagccgctgt gccgctggta cgcgcaccgc
     6961 gtgatgcagc atctgtgcac ggcgaatcgg gaatggctgg ctacgacttt gttgagcaca
     7021 accgaaagcc aatcgggata ccagcgtttc tggcgattcg tgatgccctg atgcgtgcac
     7081 cagagcctgt taccctggtg gccattggcc cgttaaccaa tattgcgctg ttactttcac
     7141 aatgcccgga atgtaaaccg tatattcgtc gtctggtgat catgggcggt tcttctggtc
     7201 gcggcaactg tacaccaaat gccgagttta atatcgctgc cgatccagaa gcggctgcct
     7261 gtgtcttccg tagcggaatc gaaatcgtca tgtgcggtct ggatgtcacc aatcaggcaa
     7321 tattaacccc tgactatctc gctactttgc cggagttgaa tcgcaccgga aaaatgcttc
     7381 atgcactgtt tagccactac cgtagcggca gtatgcaaag cggtctgcga atgcacgatc
     7441 tctgcgccat cgcctggctg gtgcgcccgg acttattcac cctcaagccg tgttttgtcg
     7501 cggtagaaac tcaaggcgaa tttacctcag gcacgacggt ggttgatatc gacggttgcc
     7561 tgggcaaacc agccaatgta aaggtggcat tggatctgga tgtgaaaggc ttccagcagt
     7621 gggtggctga ggtgctggct ctggcgtcgt aacctgtcac atgttatcgg catgcagtca
     7681 ttcatcgact catgcctttc actgatatcc ctccctgttt atcattaatt tctaattatc
     7741 agcgtttttg gctggcggcg tagcgatgcg ctggttactc tgaaaacggt ctatgcaaat
     7801 taacaaaaga gaatagctat gcatgatgca aacatccgcg ttgccatcgc gggagccggg
     7861 gggcgtatgg gccgccagtt gattcaggcg gcgctggcat tagaaggcgt gcagctgggc
     7921 gcggcgctgg agcgcgaagg atcttcttta ctgggcagcg acgccggtga gctggctggt
     7981 gcagggaaaa caggcgttac cgtgcaaagc agcctcgatg cggtaaaaga tgattttgat
     8041 gtgtttatcg attttacccg tccggaaggt acgctgaacc atctcgcttt ttgtcgccag
     8101 catggcaaag ggatggtgat cggcactacg gggtttgacg aagccggtaa acaggcgatt
     8161 cgtgacgccg ccgccgatat tgcgattgtc tttgccgcca attttagcgt tggcgttaac
     8221 gtcatgctta agctgctgga gaaagcagcc aaagtgatgg gtgactacac cgatatcgaa
     8281 attattgaag cacatcatag acataaagtt gatgcgccgt caggcaccgc actggcaatg
     8341 ggagaggcga tcgcccacgc ccttgataaa gatctgaaag attgcgcggt ctacagtcgt
     8401 gaaggccaca ccggtgaacg tgtgcctggc accattggtt ttgccaccgt gcgtgcaggt
     8461 gacatcgttg gtgaacatac cgcgatgttt gccgatattg gcgagcgtct ggagatcacc
     8521 cataaggcgt ccagccgtat gacatttgct aacggcgcgg taagatcggc tttgtggttg
     8581 agtggtaagg aaaatggtct ttttgatatg cgagatgtac ttaatctcaa taattt
//
LOCUS       DAZPDU010000172         8633 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZPDU010000172 DAZPDU010000000
VERSION     DAZPDU010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8633
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.174"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(144..2687)
                     /gene="mdoH"
                     /locus_tag="ACHURS_003650"
     CDS             complement(144..2687)
                     /gene="mdoH"
                     /locus_tag="ACHURS_003650"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFR1239284.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2680..4215)
                     /gene="mdoG"
                     /locus_tag="ACHURS_003651"
     CDS             complement(2680..4215)
                     /gene="mdoG"
                     /locus_tag="ACHURS_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFR1239285.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4609..5766
                     /gene="mdoC"
                     /locus_tag="ACHURS_003652"
     CDS             4609..5766
                     /gene="mdoC"
                     /locus_tag="ACHURS_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFR1239286.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5774..7255)
                     /gene="clsC"
                     /locus_tag="ACHURS_003653"
     CDS             complement(5774..7255)
                     /gene="clsC"
                     /locus_tag="ACHURS_003653"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFR1239287.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRQMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLSDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(7197..7730)
                     /gene="ymdB"
                     /locus_tag="ACHURS_003654"
     CDS             complement(7197..7730)
                     /gene="ymdB"
                     /locus_tag="ACHURS_003654"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFR1239288.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7825..8136)
                     /gene="ymdA"
                     /locus_tag="ACHURS_003655"
     CDS             complement(7825..8136)
                     /gene="ymdA"
                     /locus_tag="ACHURS_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFR1239289.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(8257..8589)
                     /gene="csgC"
                     /locus_tag="ACHURS_003656"
     CDS             complement(8257..8589)
                     /gene="csgC"
                     /locus_tag="ACHURS_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFR1239290.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WPPSSEKS"
BASE COUNT         2155 a         2247 c         2051 g         2180 t
ORIGIN      
        1 cataacgccg gtatttttat tggctcgttg tttttgccgg atgcgacgtg aacgctttac
       61 ctaacccaca aaagcttgca aaatcaataa attgcatgaa cgatgtaggc ctgataagcg
      121 tagcgcatca ggcaactaca tttttattgc gaagccgcat ccggtttacg caatgccagt
      181 ggattgagct ttatcccttc gtaataactc acccatgaag aatatctctc aggggaattc
      241 cagacgcgga aatgcagacg ggccatcgtc accggatcgc ttagcagcac aaggcgacga
      301 tcgcgattca gcttctctgg cgtctcgttc agcgcctgtt caacgtggcg gtcacgggcg
      361 atttccagca ccttactggc gcggtgacgc gcggtcgcca ttgcggttgc cagagcgtta
      421 aatgacgggt taaacactgc gtgcataaag ccatcgtcaa gggagcgttg gcgattcatc
      481 tcaaggaacc gatcggtatc aaccagcacc tgcggcggtg aatactcttc cgggatcagg
      541 aacagtttcc agcgtttggt gcgcagacca acggtggcac ggctggaaat caccgaaaca
      601 aacggtgaca ggatcaacga gaagacaatc ggtgccagcc agaacaggaa acgcagatcc
      661 agccacgcca tcccaacagc ccacactaac cctaacagca gctgtgagcc gtggcgtttg
      721 aacgcttcac cccaggaagt ggagtcatca tcacgctgcg gtgaattcca caccacttcc
      781 cagccaagga acgcgctgac gacgaagacc gtatggaaca gcatgcgtac cggagccagc
      841 agcacggaaa acagcacttc cagcagcaac gataatgtaa cgcgccagaa gccgccgtat
      901 tctttcgttc ctttgcacca gataagcaaa atgctcaata acttcggcag gaacaacagc
      961 accatggtcg aagcaaaaag tgcaatcgcc agctcaggac gccactgcgg ccacaccggg
     1021 aacaactgcc gtggttgcag gaagtattgc ggttcggtca acgcatgtac tacctgcaat
     1081 gcagtagaga gcgcgaggaa cataaaccac agcggagcgg agagataaga catcacgccc
     1141 gtcaggaaca ccgcacggtg aaccgggtgc atacccttca ccaggaacag acggaagttc
     1201 atcaggttac cgtggcacca tcggcggtca cgttttagct catcaagcaa gttaggcggc
     1261 aattcttcat aagaacccgg gagatcgtaa gcaatccaga ccccccaacc tgcacggcgc
     1321 atcaacgccg cttccacgaa gtcatgtgac aggattgaac cggcaaagga accttcgccc
     1381 ggcagcggag ccagtgcgca gtgctcgata aacggtttca cgcggataat cgcgttatgt
     1441 ccccagtagt gcgactcgcc aagttgccag aagtgcaaac cggctgtaaa tagtggccca
     1501 tacacgcggg tcgcgaactg ctgacagcgc gcatacagcg tatccatacc ggacgctttc
     1561 ggcgacgact gaatgatccc ggcgttcggg ttggcttcca tcaggcgcac cagcccgcac
     1621 aaacaatcac cggtcattac cgagtcagca tccagcacca ccatgtagct gtactggctg
     1681 ccccagcgac ggcagaagtc atcgatatta ccgcttttac gcttcacgcg gcgacggcgg
     1741 cggcgataga aaatctgacc ttcgccaccg acttcagcga taagctccat ccaggctttt
     1801 tgctctgcga cgcagatatc cgggttataa ctgtcactaa gaatgtagac atcaaagtgt
     1861 ttggcattcc cggtggcttt tactgattcc cacgttgcac gcaggccagc aaaaacacgg
     1921 ttcacgtctt cgttacagat aggcatgatc aacgccgtgc gatgctccgg gtttaatggt
     1981 tcatcgccaa ctgttgacgc agatatactg tatttatcgc gaccaataag cagttgcagg
     2041 aagcccatta acgccgtcca gaatccggcg gacacccaac agaacagtac cgcaaagagg
     2101 atcaggatac cggtttgcag catataaggc agaagctgca taaaggaaac ccacaaatcc
     2161 tgaccaacca tatccatagg attaatcagc gcccaaccct gataaggaag aatggtcttc
     2221 atataccagg tcgcgacgac agtttgcgcg agcgtcagga tcaacagaat gtaacggcgg
     2281 atggtaccga cggtacgcca cttttgctcg ctctcctgct cttctttggt caaacgagcc
     2341 agatagcgcg gcgtgacatc gcgtccacgc aggcgatccc agaaacggcc taccgggttg
     2401 gtacgccacg ggtcgggaaa catcgaggag cgttttgctt ctggcatcgc cttcagctga
     2461 tcgcgccctt cgtcgtcttt aattaactgt ccatcagcaa gtgaatctgg ccaggcttgt
     2521 tccagacgcg cctttaccga gccttgcggg gaatcatcct cccgcgccca ggtgcggtgt
     2581 tcggcatcca gcgcctgatg aacggcgcgg atatcagtct tcggcaatgc cgctttctcg
     2641 cttgcggcga tgggcattgc gtcaatgtac tcagttgtct tattcattgg caggtaactg
     2701 gtagctccag gtttcactca acgtctgatc ggcattcacc agcgcagcac gcatttcagt
     2761 ggttttcttg gcatctttca ctttcacacg catcaccaga cgccagcctt tggtaaccgg
     2821 gttatagcgc accgtgcttt caactatctc accattatca ccaatgctgg tttgcgctgt
     2881 gaccggggta tcctctggca gttttttcat ctctgcgccg gtaaaatcga ccacaaaggc
     2941 gatagtaccg tcaggctggc gaatcaggtt cgactgcttc acatcccccg ttgaacgacg
     3001 cgtttgttgc acccatgcgt tatctggcgc atgcagtttg tcttcatcac ggctgaaggt
     3061 gatggtgtat ttaaagttca tctctttacc cggctccggc agctgatccg gcgtccagta
     3121 agcgacgatg ttatcgttgg tttcatcgtt ggttggaatt tccaccagct caacgctgcc
     3181 tttaccccac tcccctttcg gagtcaccca tgcgcttgga cgaagatcgt aacgatcatc
     3241 gagatcttca aagcgggaga aatcacgacc gcgctgcaac agaccgaagc cttgcgggtt
     3301 ttccatcgag aagctgctaa ccgctaaatg tttcgggtta ttcaacggac gccagatcca
     3361 ttcgccatta ccggcatgga tagagagacc gttagagtcg tgcaactccg gacgatagtt
     3421 atttgcaggc gacggttggt tcggcccaaa caggaacata ctggttaacg gtgcaacccc
     3481 cagtttgccg actttatcgc gcagatagat tttcgactgc acatccacaa ccgtgtcacg
     3541 ccctggcata accacgaatt tgtaagcacc tgtcgcgcgc ggcgagtcaa gcaatgcata
     3601 aatggttaaa cgtttatcag tcggttttgg acgctcgatc cagaactctt tgaagcgtgg
     3661 aaattcttcc cccgatggca aggcggtatc aattgccagg ccgcgggcag aaaggccata
     3721 aacctgacct gcaccaatca cgcggaaata gctggccccg agcatgctga cgatttcatc
     3781 gtttttatct ttgctgttga tcgggtaaag cactttaaaa ccggcaaaac caaggtcttt
     3841 taccgtgtct ttgtcatgct gaacatcgcc gaaagtgaaa taatccgggc tgtatttgat
     3901 tcgtttgact gcggtggcag tcacttcatt tattttgacc ggggtatcga agtacatacc
     3961 ctgatggtag aactcgagtt tgaatggggt cttcagattg ttccagtacg ctttgtcatg
     4021 attaaactgg atctgctgat agtccgcgta tttcatatcg cggaaaacgg agggcaagtt
     4081 gcttttgggc gcctcatagc ctttcccggc taaggattga gcttgctttg cgacatcatc
     4141 aatactgaaa gcccagcttg aagatgtata cagggttaac attactgcag cactcaacca
     4201 acgcattttc atcatttgta gtttatgttt cataattagt aagcacttcc ccctttgtgt
     4261 gcttatatcg atccgatcta ttttaatgga taaccagcca ttccgacaac agaatcccgg
     4321 tatcgttcag cacgctggct catggattaa gcacctgatt gcaaccgttt tcactttccg
     4381 tgcttctcct aaagacagaa gcatcgactt cgtgtagggt ttgccagcaa ttttatttat
     4441 caaccgtctt acagacgctt ccattctggt tagtggtagc gatgatgcta tgcataaaaa
     4501 ccttgaagcc tgcgcctggc gcggtcaggt cggtgtcgct atggcaacaa gcctaaaggc
     4561 ataacccgac agggataaga cgaaaaatcc gagattagtt aaccatatat gaatccagta
     4621 cccgcgcaac gtgaatattt cctcgactcc atccgcgcct ggctgatgtt gttagggatc
     4681 ccttttcata tttctttaat ctattcgagc catacatggc atgtgaatag cgccgaaccg
     4741 tcattgtggc tgaccctttt taatgacttc atccactcgt tccgcatgca ggtatttttc
     4801 gttatatccg gctacttttc ctacatgctt tttttacgct atcccttgaa aaaatggtgg
     4861 aaagtacgtg tcgaacgtgt aggtatcccg atgttaacag ccatcccctt actgacattg
     4921 ccgcaattta ttatgctgca atacgtcaaa gggaaagcgg aaagttggcc tgggctgtca
     4981 ttgtatgaca aatataatac gttggcctgg gaattaatat cacacctgtg gtttttactg
     5041 gtgttagtag tcatgacgac gctgtgcgta tggatattta aacgcatcag aaataattta
     5101 gaaaattctg ataaaacgaa taaaaaattc tcgatggtaa aactatcggt gattttttta
     5161 tgcctcggca tcggctatgc ggtaataaga agaacgattt ttattgtgta tccgcccatt
     5221 ctgagtaatg gcatgttcaa ttttattgtc atgcaaacgc tattttattt gccgttcttt
     5281 atcctcggtg cactggcttt cattttccct catcttaaag ccttgtttac cacgccgtct
     5341 cgtggctgta cccttgcagc agcattggca tttgtcgctt atttactcaa ccagcgctat
     5401 ggcagtggcg atgcctggat gtacgaaacc gagtcggtga tcaccatggt cctcggtctg
     5461 tggatggtga atgtggtctt ctcctttggc caccgtttgc ttaacttcca gtcagcgcgg
     5521 gtgacttact ttgttaacgc atcgctattt atctatctgg ttcaccaccc gttaacgctg
     5581 tttttcggcg catacattac accgcacatc acctccaact ggcttggttt tctctgtggc
     5641 ctgatattcg tagtagggat tgcgataatt ctgtatgaaa ttcatttacg catcccgtta
     5701 ctgaagtttt tgttttctgg taaaccggtt gttaagcgtg agaacgataa agcaccagcc
     5761 cgttaagcca catttacaat aaccattcca cgggcaatat cgacgccagt ctgaccataa
     5821 cccgcttcca gaaactggtg gcgggttctt ttttgagaat aatctctttc tttgcatgac
     5881 gatcaaccca gttgatccgt ccccacctgt ccagacggag ctgccaggcc gcatcatact
     5941 ggctctgaat aaagcgttta tcaattaact gtgccagcgt ttcgctctct atcacgaagc
     6001 ccatttcagt attgagcaat gttgaacgcg gatcgaaatt gaaagagccg ataaacaccg
     6061 ttttaccatc gatgctaaaa gttttagcat gcaggctggc tccggaatta ccggttatgc
     6121 cgcgatcgtg taacgtacta ctttgttcac gcgtcggctt gagttcatat aattccacgc
     6181 catagcggag caattttttg cgccagcgcg cgtatccggc atggacgaca gcaacatcgt
     6241 tagcggcaag agaattggtt aggatcgcaa tctttacccc ttttctcacc atccgtaaga
     6301 gttgcgccac acctgcgcgt gtcggtacaa aataggaaga gataatatcg atgcgttcac
     6361 tgggtgagcc catgatatcg gacaggcgct gcggtaacag tgaatgccgt tttgccttgc
     6421 cctccccttt cgccggatca tcacttaata aacgtgtttt cgcccagata agcggcaacg
     6481 ttccatcaac cagatgattt ataaacggac tggattccat ttggcgtaag taacgatgcg
     6541 tcatggcatc gttatgccag gaggcgggta actcgatgcg atccgccatt tcaccttccg
     6601 ggacatccag cacctgctgt aagggtgaaa ccgatttgca ataccagtag cgggcgaaat
     6661 catcggcaac gtcctctacc acgggtccta ttgccataac atctaaatcc gaaaaaagtg
     6721 gctcttcccc tgccccaaaa taggcatcac caatatttcg tcctcccacc agggtaacca
     6781 cgccatcgac agtgaaactt ttattgtgca tacggcgatt gagacgggaa aagtcggtga
     6841 tataaccaag cggacgcagc aagcgaaacg agaaaggatt aaaaagccgg acttcaatgc
     6901 ggggatggct gtcaagcaag cgtaaaatat cgtcaagtcc gggcgtattg ttgtcgtcca
     6961 gcaacaaacg gacgcgaacg ccacgctttg ctgcggctaa cagggcggaa aacagtaacc
     7021 gacccgacat atcgtcctgc cagatgtaat actgaacatc cagcgtatgc tctgccattt
     7081 cggccagacg ataccgggcg gcaaacgcat ccagactttt ctccagaggg aaaagaccac
     7141 actgaccggg atgttgcgaa cacagtggca gcaccgcgct cgccagccgg ggcaaatcat
     7201 tcatctcctt gttgggtaag gagtctttcg tagaggtggg cgttttcttc atcataacag
     7261 acaaagtata cctgttcggg taaagcgtga cgggtaataa attctgaaac ggttttaact
     7321 gcgatttcag ccgctgccgc acgagggtaa ccataaaccc cagtactgat tgcaggaaaa
     7381 gccactgacg tatagctgtt tgccgccacc agtcgcaggc tattcagata ggcatcctgc
     7441 aaaagctggt cttcgttttg ttcaccacca cgccagactg gcccgacggt gtgtactacg
     7501 gctttagcgg gaagatcgcc tgcaagcgta ataacggcat gccccgtagg gcaatcgccc
     7561 tgctgttgcc tgacttttaa acaggcatcc agcagggccg gacccgctgc gcgatgaatg
     7621 gccccatcga cgccgccgcc tcccattaat gacggattag ccgcattcac aatcacatca
     7681 acggccagtt tggtaatatc accctgcaca acatgaatac gcgttttcat aacgcctcct
     7741 tacaccttct tgtttcttaa gtgtacagca ggctttttgg ttaaatcgca tttttctgct
     7801 cagcaacgtg cgttgttcag aaacttagtt atattcgatg ttcattacag ccagcttttt
     7861 ctgtttatca agatactgca cttttactga ggctatttgc gcgtttttaa cattaccgct
     7921 cattaacgct ttgctggaat aggttttgcc cggaacagaa ccattctgtg ggcagttcat
     7981 ttctacgggt gatgactggg tgctgacgtc acatgccggt tccacaattt ggccataaaa
     8041 atggatcaca cccccttgcg cggtgcttcc tgcgatggca ataaaaggaa aaaacaaact
     8101 gccgagcata agagagttta aaaaaggtct gaacatttat tctatttcct caatgattag
     8161 tcatccttga gggttgtgtt atccatactt tcaggttggc tgattcgcag cagaccattc
     8221 tctccagatt catcttatgc tcgatatttc aacaaattaa gacttttctg aagagggcgg
     8281 ccattgttgt gataaatgaa gtgcctgtcc atcagaaaca gtaacaacta ttttcacccg
     8341 atcttccggg gaaatattta aactcaactt cgtcaaagca ataggttgat tagcaggcaa
     8401 tgagagggtc ttttcttgct tcgtctgact ttgccctgaa ctgccttcgc gcagggacaa
     8461 tatttgtact ctgcacagac aagattgagt aagagtgact tcaggaataa tggtatacac
     8521 atccccttgc tgggtcgtat taaaggttat ctggctggaa agtgccgcaa ggagtaataa
     8581 cgcattcata ttcttctccc gaaaaaaaca gggcttgcgc cctgtttctg taa
//
LOCUS       DAZPDU010000173         8602 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZPDU010000173 DAZPDU010000000
VERSION     DAZPDU010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8602
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.175"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(181..1845)
                     /gene="glnS"
                     /locus_tag="ACHURS_003657"
     CDS             complement(181..1845)
                     /gene="glnS"
                     /locus_tag="ACHURS_003657"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFR1239291.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(2104..4050)
                     /gene="nagE"
                     /locus_tag="ACHURS_003658"
     CDS             complement(2104..4050)
                     /gene="nagE"
                     /locus_tag="ACHURS_003658"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFR1239292.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDAMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVSDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGNVVAGQTPLYEIKK"
     gene            4383..5183
                     /gene="nagB"
                     /locus_tag="ACHURS_003659"
     CDS             4383..5183
                     /gene="nagB"
                     /locus_tag="ACHURS_003659"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFR1239293.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5243..6391
                     /gene="nagA"
                     /locus_tag="ACHURS_003660"
     CDS             5243..6391
                     /gene="nagA"
                     /locus_tag="ACHURS_003660"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFR1239294.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLARHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTSFTPDFKITKTIVNGNEVVTQ"
     gene            6400..7620
                     /gene="nagC"
                     /locus_tag="ACHURS_003661"
                     /gene_synonym="nagR"
     CDS             6400..7620
                     /gene="nagC"
                     /locus_tag="ACHURS_003661"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFR1239295.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7668..8420
                     /gene="nagD"
                     /locus_tag="ACHURS_003662"
     CDS             7668..8420
                     /gene="nagD"
                     /locus_tag="ACHURS_003662"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFR1239296.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            complement(8471..8602)
                     /locus_tag="ACHURS_003663"
                     /pseudo
     CDS             complement(8471..8602)
                     /locus_tag="ACHURS_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2101 a         2271 c         2194 g         2036 t
ORIGIN      
        1 cttgccggat gcggcgtgaa cgccttaacc ggcctacaaa agcacgcaaa ctcaatatat
       61 tgcagagatc atgtaggcct gataagcgta gtgcatcagg caatttagcg tttgaaactg
      121 tttgtctgtt ccttgccgga tgcgacgtaa acgccccatc cggcatagcg aaacttaaaa
      181 ttactcgcct actttcgccc aggtatcacg cagcccaacg gtgcggttaa ataccggttt
      241 ttccgccgta gaatggcggc tatcgaggca gaagtaacct tcacgctcaa actggaatgc
      301 tttacccgca accgcatctt tcagcgacgg ttcagcaaag ccctgtttga tcaccagcga
      361 ttccgggtta atcaccgaca ggaaatcatc cgcagcacca gggttcggca cgctgaacag
      421 acggtcgtac agacggattt caaccggcag cgcatgtgcc gcgctcaccc agtgaataac
      481 acctttgact ttacgaccat ctgccggatc tttgcttaag gtgtcggcgt cataagtaca
      541 gaagatggtg gtgatattgc cttcggcatc tttctccacg cgttctgcct taatgacgta
      601 agcattacgc agacgcactt ctttacccag caccagacgt ttgtactgct tgttagcttc
      661 ttcgcggaaa tcggcgcgat cgatccaaat ctcaccgcta aacggcacct ggcggctacc
      721 catttccggt ttgttcggat ggttcggcat ggtaaccatt tcgccttcgc cctgatagtt
      781 ttcgataacc agtttcaccg gatcgataac cgccattgcg cgcggggcgt tttcgttgag
      841 atcttcacgg atgcaggatt ccagcgacgc catctcaatg gtgttgtcct gcttggtcac
      901 gccgatgcgt ttgcagaact cacgaataga agcagcagtg taaccacgac gacgcagacc
      961 ggaaatggtc ggcatacgcg ggtcatccca gccttcaacg tgcttgtcgg tcaccagcag
     1021 gttcagctta cgcttggaca tcacagtgta ttccagattc aggcgcgaga attcgtactg
     1081 gcgcgggtga acaggaatgg tgatgttgtc cagcacccag tcgtacaggc gacggttgtc
     1141 ctggaactca agcgtacaca gagagtgcgt aataccttcc agggcatcgc tgatgcagtg
     1201 ggtgaagtcg tacatcgggt agatgcacca cttgttgcca gtctggtggt gttcagcaaa
     1261 tttaatgcgg tacagcaccg gatcgcgcat cacgataaac ggtgaagcca tgtcgatttt
     1321 cgcacgcagg caggctttac cttcttcaaa accaccggca cgcatttttt cgaacagcgc
     1381 caggttctct tcaacgctgc ggtcgcggta cgggctgttt ttacccggtt gcgtcagggt
     1441 gccgcggtat tcgcggatct gttccggcgt cagttcatca acgtacgcca ggcctttatt
     1501 gatcagttcg atcgcatagg cgtggagctg atcaaaataa tcggaggagt aacggacgtt
     1561 accagaccag tgaaaaccta accactctac gtcgtttttg atcgactcaa catactcgat
     1621 atcttctttt accgggttag tgtcgtcgaa acgcaggttg cactggcctt tatagtcctg
     1681 ggcgatcccg aagttcaggc agatagattt cgcatggcca atatgcagat agccattcgg
     1741 ctccggcggg aaacgggtgt gtactgtggt gtgcttacca ctggccagat cttcatcgat
     1801 gatctgacgg ataaagttag tcgggcgggc ttctgcctca ctcatcgtgg attcctcaaa
     1861 gcgtaaacaa cgtataacgg cgtatgatct tataagcggg acaggctgac aactgttagt
     1921 tagcgaaaat cgaaaatctc actgagatta tggggatgat tgatgaaaaa aaaagcggca
     1981 gagaagatct ccaccgcttt tgccagtccc gcgtcattgt tggatgcgcg tcttatccaa
     2041 ccaggactga gcttttgtag gcctgataag acgcgtcaag cgtcgcatca ggcataaagc
     2101 agattacttt ttgatttcat acagcggtgt ttgacccgcc acaacattgc cctgagcttt
     2161 aatgatcaag ccactgaaat cgtcgatatt gctgcaaacc accgggctaa tcatcgaacg
     2221 ggcgttagcg ttcaggtaat ccagatccat ttccagaatc ggttgccctg cgctcacctg
     2281 cgcgccctct tccaccagac gtttaaagcc tttaccttcc agcgctacgg tgtcgatacc
     2341 catgtggacg acaatctccg cgcctttttc ggtttctagg cagaacgcgt ggttggtgtt
     2401 gaagattttc acgattgtcc ctgcggctgg tgatacgacg attttatctg tcggtttcac
     2461 cgccacaccg tcacccaccg ctttgctggc gaatgcttcg tcagaaacct gatccagtgc
     2521 cacgacatca ccggtaatcg gcgataccag ctccgcgata gataccgcgt ttggtacagc
     2581 ctgcggtttt gctacaggag cggcagttgc cggagttgct tcagctgacg cagctgctac
     2641 cgggccacgg gcaacgactt tcttcatcgc atcgccaatg gattctgctt tcgcgccaac
     2701 aatcacctga atggtctgtt tgttcagttt tactacacca gaagcaccca gacgtttaca
     2761 catcgcatcg ttgacgcgag ctgagtcagc cacggtaagg cgcagacggg taatacaggc
     2821 gtcaatcgct ttcaggttgt cagtaccgcc aaccgcagca atatagttgg ttgccagttg
     2881 agtcagacct tcttcagtat tgctgttagc ctcttcagtt acgatctcgt cttctttatc
     2941 ttcacgaccc ggcgttttca ggttgaacat gcggataacc aaactgaaca ccacgaagta
     3001 gatagcgaag aagacaacac ccatcaccag cagcatccag acgttctggc tggcggccgg
     3061 caggttatac atcaacgcgt agtcgatagc ccccgcagag aaagagaagc cagcatggat
     3121 acccagcagc gttgccacaa acaggctgat accggtcagc agtgcgtgca ggaggtacag
     3181 cagcggagca aggaacatga acaggaattc cagcggctca gtcacaccgg tcaggaacgc
     3241 agtaacagca acagaaagca gcatcccgcc aaccatcgga cgacgctctt tcggtgctgc
     3301 gaagtacatc gccagcgccg cacccggcag accgaacatc atgatcggga agaagccgga
     3361 catgaacatc cccgcggtgc cgtcaccagc gtagaaacgg ttgatgtcgc cgtggaaaac
     3421 cgtacccgcc gcgttggtga attcaccaat ctggaaccag gcgatggtgt tcagcacctg
     3481 atgcagaccg gttgggatca gcagacggtt gatgaaacca aagataccgg aacccagcgc
     3541 gcctgcagaa acgatccact cgccgcctgc atggatagcg tgctgtactg gcggccagac
     3601 gtaaccaaaa atggccgcca gcaccaggca gaagaagccg gtggcaatcg gcacaaagcg
     3661 tttgccgccg aagaagctca ggaagtccgg cagtttaata tcggaccaac ggttataggc
     3721 tgcgccacca accagaccgg taatgatacc cgccagtaca cccatgttaa tttctgggtt
     3781 gatggtcacc atcgctttgg ttaacacaaa gtaacctacc gcacccgcca gcgccgccgc
     3841 acctgcgctg tctttcgacc agctggatgc cacaccgatg gcgaagatta atgcgaggtt
     3901 atcaaaaatc gcaccgcccg cctgggcaat aaacgcaacg ttaagtaaat ctggctgacc
     3961 gaatcgcagc aacagtgccg ccaccggcag caccgcgata gggagctgta acgccctacc
     4021 gagtcgctgg aaaaaaccta aaatattcat cttattcccc ctacgagaac cctatttggc
     4081 tcgtttaaag ccgtattttt attttgctgc aaattgtact gccgatgttc tgtaatcaga
     4141 ttgttagatc atctgctaca gagtgtgtga aaatttaatt cgtatcgcaa attaaacgcg
     4201 tgccttttgt gagttttgtc accaaatatc gttatcatca ctccctttta ctggctaaag
     4261 cagaaaactt attttatcat tcaaaaaatc aggtcggatt gacgcctgtc tgcgcaaatc
     4321 caggttacgc ttaaagatgc ctaatccgcc aacggcttac attttactta ttgaggtgaa
     4381 taatgagact gatcccccta actaccgctg aacaggtcgg caaatgggct gctcgccata
     4441 tcgtcaatcg tatcaatgcg ttcaaaccga ctgccgatcg tccgtttgtt ctgggcctgc
     4501 cgactggcgg cacgccgatg accacctata aagcgttagt cgaaatgcat aaagcaggcc
     4561 aggtcagctt taagcacgtt gtcaccttca acatggacga atatgtcggt ctgccgaaag
     4621 agcatccgga aagctactac agctttatgc accgtaattt cttcgatcac gttgatattc
     4681 cagcagaaaa catcaacctt ctcaacggca acgccccgga tatcgacgcc gaatgccgcc
     4741 agtatgaaga aaaaatccgt tcttacggaa aaattcatct gtttatgggc ggtgtaggta
     4801 acgacggtca tattgcattt aacgaaccgg cgtcttctct ggcttctcgt actcgtatca
     4861 aaaccctgac tcatgacact cgcgtcgcga actctcgttt ctttgataac gatgtcaatc
     4921 aggtgccaaa atatgccctg actgtcggtg ttggtacatt gctggatgcc gaagaagtga
     4981 tgattctggt gctgggtagc cagaaagcac tggcactgca ggctgcagtt gaaggttgcg
     5041 tgaaccacat gtggaccatc agctgtctgc aactgcatcc aaaagcgatc atggtgtgcg
     5101 atgaaccttc caccatggag ctgaaagtta agactttaag atatttcaat gaattagaag
     5161 cagaaaatat caaaggtctg taattgttat ccctgccctg gctccttgct cagggcaata
     5221 ttttttaaaa tcggggggca gaatgtatgc attaacccag ggccggatct ttaccggcca
     5281 cgaatttctt gatgaccacg cggttgttat cgctgatggc ctgattaaaa gcgtctgtcc
     5341 ggtagcggaa ctgccgccag agatcgaaca acgttcactg aacggggcca ttctctcccc
     5401 cggttttatc gatgtgcagt taaacggctg cggcggcgta cagtttaacg acaccgctga
     5461 agcggtcagc gtggaaacgc tggaaatcat gcagaaagcc aatgagaaat caggctgtac
     5521 taactatctg ccgacgctta tcaccaccag cgatgagctg atgaaacagg gcgtgcgcgt
     5581 tatgcgcgag tacctggcaa gacatccgaa tcaggcgtta ggtctgcatc tggaaggtcc
     5641 gtggctgaat ctggtaaaaa aaggcaccca taatccgaat tttgtgcgta agcctgatgc
     5701 cgcgctggtc gatttcctgt gtgaaaacgc cgacgtcatt accaaagtga ccctggcacc
     5761 ggaaatggtt cctgcggaag tcatcagcaa actggcaaat gccgggattg tggtttctgc
     5821 cggtcactcc aacgcgacgt tgaaagaagc gaaagccggt ttccgcgcgg ggattacctt
     5881 tgccacccat ctgtacaacg cgatgccgta tattaccggt cgtgaaccgg gcctggcggg
     5941 cgcgatcctc gacgaagctg acatttattg cggtattatc gctgatggcc tgcatgttga
     6001 ttacgccaac attcgtaacg ctaaacgcct taaaggcgac aaactgtgtc tggttaccga
     6061 cgccaccgcg ccagcaggtg ccaacattga acagttcatt tttgcgggta aaacaatata
     6121 ctaccgtaac ggactttgtg tggatgagaa cggtacgtta agcggttcat ccttaaccat
     6181 gattgaaggc gtgcgtaatc tggtcgaaca ttgcggtatc gcactggatg aagtgctgcg
     6241 tatggcgacg ctctatccgg cgcgtgcgat tggcgttgag aaacgtctcg gcacactcgc
     6301 cgcaggtaaa gtagccaacc tgacctcatt cacacctgat tttaaaatca ccaagaccat
     6361 cgttaacggt aacgaggtcg taactcaata agagaaagta tgacaccagg cggacaagct
     6421 cagataggta atgttgatct cgtaaaacag cttaacagcg cggcggttta tcgcctgatt
     6481 gaccagtacg ggccaatctc gcggattcag attgccgagc aaagccagct tgcccccgcc
     6541 agcgtaacca aaattacgcg tcagcttatc gaacgcgggc tgatcaaaga agttgatcag
     6601 caggcctcca ccgggggccg ccgcgctatc tccatcgtca ccgaaacccg caatttccac
     6661 gcaatcggcg tacggcttgg tcgtcacgac gccaccatca ccctgtttga tctcagcagc
     6721 aaagtgctgg cagaagaaca ttacccgctg ccggaacgta cccagcaaac gctggaacat
     6781 gccctgttga atgccattgc tcagtttatt gatagctacc agcgcaaact gcgcgagctg
     6841 atcgcgattt cggtgatcct gccagggctt gttgacccgg acagcggcaa aattcattac
     6901 atgccgcata ttcaggtaga aaactggggg ctggtagaag ctctggaaga acgttttaaa
     6961 gtgacctgtt tcgttggtca cgatatccgt agtctggcgc tggcggagca ctacttcggt
     7021 gcaagtcagg attgcgaaga ctccattctg gtgcgtgtcc atcgcggaac cggggccggg
     7081 attatctcta acgggcgcat ttttattggc cgcaacggta acgtcggtga aattggccat
     7141 attcaggtcg aaccgctggg tgaacgctgc cactgcggca actttggctg cctggaaact
     7201 atcgccgcca acgctgccat tgaacaacgg gtgttgaatc tgttaaagca gggctaccag
     7261 agccgcgtgc cgctggacga ctgcaccatc aaaactatct gcaaagccgc gaacaaaggc
     7321 gatagtctgg cgtcggaagt aattgagtat gtcggtcgtc atctgggtaa aaccatcgcc
     7381 attgctatca acttatttaa tccgcaaaaa attgttattg ccggtgaaat caccgaagcc
     7441 gataaagtgc tgctccctgc tattgaaagc tgcattaata cccaggcgct gaaggcgttt
     7501 cgtactaatc tgccggtggt acgttctgag ctggatcacc gctcggcaat cggcgctttt
     7561 gcgctggtaa aacgcgccat gctcaacggt attttgctcc agcatttgct ggaaaattaa
     7621 tgtgctttta tagtggcgct tattgttgtc aatattctgg gtagtccatg accattaaaa
     7681 atgtaatttg cgatatcgac ggcgtgctga tgcacgataa cgtcgccgta ccgggtgcag
     7741 cggaattttt gcacgggatt atggataaag gcctgccgct ggtgttgctg accaactatc
     7801 cttcgcagac cgggcaagat ctggcgaacc gctttgccac tgccggtgtc gatgtgccgg
     7861 atagcgtgtt ttatacctct gcgatggcga ccgccgattt cctgcgtcgc caggaaggca
     7921 agaaagcata tgtggtgggc gaaggcgcac tgattcatga actgtacaaa gccggtttca
     7981 ctattaccga tgtgaaccct gatttcgtga ttgttggcga aacgcgttcc tacaactggg
     8041 acatgatgca taaagcggcc tatttcgtcg ctaacggtgc acgctttatc gccaccaacc
     8101 cggacaccca cgggcgcggt ttttatcccg cttgtggcgc gttgtgtgca gggattgaga
     8161 aaatctccgg gcgcaaaccg ttctatgttg gtaagcccag cccgtggatc atccgcgcag
     8221 cattaaacaa aatgcaggcg cattcggaag aaacggtgat tgtcggcgat aacttgcgca
     8281 ccgacattct ggcgggcttc caggcaggtc tggaaacgat tctggtgctt tctggtgttt
     8341 catcgctcga cgatatcgac agtatgccgt tccgcccaag ctggatttac ccgtcagtcg
     8401 ctgaaatcga cgttatctga ataccaccca cgtctgcgga cgtgggtggc tctgatgaca
     8461 aacgccaaac tgcctgatgc gctacgctta tcaggcctac gcagctcctg caatatattg
     8521 aattttcatg cttttgtagg ccggataagg cgttcacgcc gcatccggca tgaacaaatc
     8581 gcactttatc agcaatttgg cc
//
LOCUS       DAZPDU010000174         8533 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZPDU010000174 DAZPDU010000000
VERSION     DAZPDU010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8533
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.176"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(7..1344)
                     /gene="adiC"
                     /locus_tag="ACHURS_003664"
     CDS             complement(7..1344)
                     /gene="adiC"
                     /locus_tag="ACHURS_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFR1239297.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            complement(1481..2242)
                     /gene="adiY"
                     /locus_tag="ACHURS_003665"
     CDS             complement(1481..2242)
                     /gene="adiY"
                     /locus_tag="ACHURS_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFR1239298.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            complement(2567..4834)
                     /gene="adiA"
                     /locus_tag="ACHURS_003666"
     CDS             complement(2567..4834)
                     /gene="adiA"
                     /locus_tag="ACHURS_003666"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFR1239299.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLSVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPRFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(5033..5941)
                     /gene="melR"
                     /locus_tag="ACHURS_003667"
     CDS             complement(5033..5941)
                     /gene="melR"
                     /locus_tag="ACHURS_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFR1239300.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGNITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            6224..7579
                     /gene="melA"
                     /locus_tag="ACHURS_003668"
     CDS             6224..7579
                     /gene="melA"
                     /locus_tag="ACHURS_003668"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFR1239301.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPSTLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            7682..8533
                     /gene="melB"
                     /locus_tag="ACHURS_003669"
                     /pseudo
     CDS             7682..8533
                     /gene="melB"
                     /locus_tag="ACHURS_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
BASE COUNT         2118 a         2023 c         2196 g         2196 t
ORIGIN      
        1 ggggaattaa tctttgctta ttggtgcatc taagggatac gggtttttat gtagccggtt
       61 gtaattcagc gcatacatgg cggtgatgac catcagggtg acaaatgacc acataacctc
      121 tttcgccccg gaccccacca cggcccagat gcagtagagg aaggcaatgg tagtaacagc
      181 cagatatgcc gggcgtgctt taccaaagtg accgtggccg agcagcagta acgccgcaca
      241 ggtgtaaaga tatggcacca gtgtaaagat gaccgacacg gaagatacca gaccgaactc
      301 tttggttgcg tttggcgaaa tgctactgag ctggaagatg gtcatcaaaa taccgacgat
      361 aatcagcccc gccactggcg tacccgcttt atttacacgg gcaaaaatcg gtgggaacag
      421 tccgtcatcg gcagcggctt tcgccgtttg acccgccagc aacgtccagc cgcccagtga
      481 acctaagcaa cccgcagctg cgcagaagga aacaatggcc ccagcggtgt cacccaacgc
      541 catccgtgcg gcatcaccga atggcgaggc agaaacgcgc agtgcggcat tagggatcat
      601 ccccataatc gcggtggtgg agagcacata gcaaacggcg gcaatcaata ccccaccaat
      661 ggtggcgata gggacattgc gtttcgggtt tttcaccaca cctgcggcaa cggaggcact
      721 ttccacaccg atgaacgacc acagcgtaac gttaagggta ctttgaatcg caccgaaggt
      781 gcccaggccg ctgacgttcc atgccgccat ataggtttca ccacggaacc agaaccagcc
      841 aaatacggca atcccgacga tggggatcag cgccagcacg gtggcaacgg cctgcacacg
      901 ggtgatcatt ttcggaccga caatgttcag caggacgaag atccacagca ccacgacgca
      961 ggtgatggtt aataccaatg gatctttcag aatcgggaag aagtaactta aatatcctac
     1021 gccaatgacc accatagcga tattgccgat ccagcaggcc agccagtaga ggacgttggt
     1081 ttgataaccg agaaacgggc caaagcagcg gcgggcgtaa gcgtaagaac caccaggact
     1141 tggatcgagg aacgacattt tggcgtatac catcgagagc cccagcgcac cgataatcgt
     1201 caccaaccat ccataaatgg caatcccgcc agtggaggcc aggtttgcag gtaacagaaa
     1261 aacacctgac cccataatat tccccgacac catcagggtg acggggatta agcccacttt
     1321 gtgagcatca gcatccgaag acataattaa actcctgcga aggcgagctt cgtgacaata
     1381 aattacgtca tatcatacgc ctgcattcga tagttgaatt attatcgacc gggttatcgc
     1441 tgatgccgtt attaatatag ctcttcgtta aggttaaaaa tcaggcgaca gttcgttctc
     1501 tgtgctgact aacataatgc agcggcgtca taccgtagaa gtctttaaag acagaaataa
     1561 agtacgacgt actgttgtag ccgcaggact gtgatacctg agagatattt ttaccgtcca
     1621 tcattaattc atttacggca taacgcatgc ggcaggtggt gattatttgg ctataactgg
     1681 tgttttcgct tttcagcttt ttctttaaca gacttgggct aagacataaa caactggcta
     1741 ccatactaag attccagtct ttgtgaatat cgctttcaat aatttgataa acgctgtcac
     1801 ttacacaatt acgtaacata tacatcatta atgaaaggaa ttttttactg tcgagaaaac
     1861 gggatagcac cgtgaataat aatgcgcgtg ttcgttcctt ttctgactca gtttctgcgg
     1921 gcatcatgct gtgttgcgcg gcaacgcgaa agacgtctgg cgtcaggcat ggcagggtga
     1981 gtatgggcgt agcgctacgt tgccatactg gtatttgcga gagatcttta ttcagaaacc
     2041 ggaggtaatc tttgatgatg tcgtgactaa tatgagcaac caaagtgttg ttaagagagg
     2101 agacgtcgat aatattattt tcacaattta ataacgccat atggttagct ttaaggctaa
     2161 taggttcttt cccattcacc cttatccaga catctttttc agtcaataaa acaatacaag
     2221 gttggtcgct gcaaatcctc atacatgtac tcctgagtgc gaataaaaga ccagttctcg
     2281 ctaaagcaaa gcgataccgg gaaatggtta tataattgta tgatgaatat aaacatgcat
     2341 aaaaataacg agttttacta atattacaaa tttttatagt aatttctcat gtacatattt
     2401 ttttacagga taaataacag gcaaaaaaaa gcgcggggtg gggccgcgca aaaagtatga
     2461 catttttgtt tgcagcaatt tttatgaaaa cgttgccata ctcaaaaggc ctaaggccag
     2521 gcggcgtaat gttatttaaa caattacgcc ttcagcggaa tagtggttac gctttcacac
     2581 acataacgtg gtaaataccg tcaataattt cagtcccttc agtttcgtgt tcaaatctag
     2641 ggaaatggtg gtcccaggat tgcagcgagc gtaaataact tacttgcgga ctgtttttat
     2701 cgccgaagtt ttcaccagac agcagcatcg ggattcctgg cggatatggg ataactgagt
     2761 ttgccgcgat gcgtcctggc agattttcaa tggataccag ttcgacattg ttgtcgacaa
     2821 tcgcgttgta cgcttcacgc ggggtgattt ccgccaccga caggccggaa taggcttcgt
     2881 tcaaccgtgc gccagggttg ttttctttca gccaggcaaa catggtgtca cccagatcgt
     2941 gaatccccat gttcgcgtaa gtgtcaggat attgttcaac aagttccggc atcacctgcg
     3001 ccagcggtgt gttggcgtca tagtggcgtt tgaaggagca aagggtgtta accagagttc
     3061 cccatttccc acgggttacg cccatagaga acaggaacat aatttggaag tcagtggtgc
     3121 gggtaggcac aatgccgtgg cgaccaagcc aggcagtgac cagcgccgcc ggaacaccgg
     3181 tttcttccag ttcaccatct tcacccattc ccggagcaag gatgctgact ttaatcgggt
     3241 cgagcatact ccagttatcc ggaatatctt tgaagccgtg ccagctttcg cccggatgca
     3301 ttacccagca gtcctgaacg gtggtcagca gtttggttgg tgcgtcagca aagtcatagg
     3361 ttttgccggt ttgtgggtcg gtgacgactt ctttgttcca cggtttgaag aaccagctac
     3421 cgtcagcggt gaactcttta tacagccgcg ccatcgcctg acggaaatca accgcttcgt
     3481 cgatcacttc ctgcgtcagt gacaggccgc tgttgccgtc catcatcgac accgccacgt
     3541 cgttggatgc gcagatggca tacagcgggg aggtggtggc atgcatcatg taggcctggt
     3601 tgaagcggga gaagttaatc gccccacgac cttcacgtac atgaatataa gaagcctgtg
     3661 aaagcgcatt cagcagtttg tgggtggagt gggtggcgaa aacggtagga ccgttgtgat
     3721 cgccaggttc gccgcgcatg gcatagtgat cggcatagat cgggttgaaa cgtgcatagc
     3781 cgtaccaggc ttcgtcaaag tgcagacgat cggaggtttt ttccagcaga tcctgcgctt
     3841 ctttagcgtt ataacacacg ccgtcatagg tgcagttggt caccacgcag taagacggtt
     3901 tttgcccggc tttgtcttta gtcagcgggc tttcactgat tttcttctgc aaggtttcag
     3961 gttgcatttc ctgcggatag attggcccga taatgccgta gcggttgcgg cttggcacca
     4021 tgtagaccgg tttcgcgcct gtcagcatca agccttgttc gatggattta tggcagttac
     4081 ggtcaacgac cacgacatcg ttatcggtca tgcaagcctg catgatggtg cggttagagc
     4141 cggaagtacc gacgactacc gaccaggagc gatcggcacc aaatacgcgt gcggcatatt
     4201 tttcgctttc gccaaatgcg ccagtatggt caagcaaaga accgagggaa gttcgttcga
     4261 tgcccatatc ggtgcggaac agattttcac catagtagtc atggtagaaa cgtccggcgg
     4321 gtgttttggt aaaaccaacg ccgccctggt ggcctggcgc tgcccaggaa tattcatgga
     4381 tgtcgctata tttcatcagc gcgctgaaca gtggcggcaa cagctgctgg cggtagcggg
     4441 tcatcgcggc aacagcgcgt ccggcgataa agtcggcggt atcttccaga atccaggcga
     4501 attcatcgac aagctccagc aggtcgcgat ccattgcggc gagggctttt tcccgatcgc
     4561 ccaacaggaa gaccggcacg ttttgttggc gctcatgaag cttaccgatc aattgtctga
     4621 cgttttgatg ttcgtccgga tgttccattt gatagctgaa catcaggcag tcaatggctt
     4681 cgtttgaaga gagaatggca aaaccatcat caaaggaggt ggatttaatc acggtaacat
     4741 tttgctggct taaagcatct gccagacgct caacggcgtt accgacccag gtgtcttgat
     4801 ggagaaactc gctttcaaca attaatactt tcatcattgc ttacccggtt atgaaggaat
     4861 cttcgttgcg ggcaagtatc ttccggcctt ttcgggctgt aaagcgcgtg aaaaacaaac
     4921 atttttgaaa tatttatttt tataaacaat gcgttatgtg attattttaa attattttca
     4981 ctgcttatta tcgacggcta aactattttt ttggctgata ctgatatcgt ctttagccgg
     5041 gaaacgtctg gcggcgctgt tggctaagtt tgcggtattg ttgcggcgac atgccgacat
     5101 atttgccgaa cgtgctgtaa aaacgactac ttgaacgaaa gcctgccgtc agggcaatat
     5161 cgagaatact tttatcggta tcgctcagta acgcgcgaac gtggttgatg cgcatcgcgg
     5221 taatgtactg tttcatcgtc aattgcatga cccgctggaa tatccccatt gcatagttgg
     5281 cgttaagttt gacgtgctca gccacatcgt tgatggtcag cgcctgatca tagttttcgg
     5341 caataaagcc cagcatctgg ctaacataaa attgcgcatg gcgcgagacg ctgtttttgt
     5401 gtgtgcgcga ggttttattg accagaatcg gttcccagcc agagaggcta aatcgcttga
     5461 gcatcaggcc aatttcatca atggcgagct ggcgaatttg ctcgttcgga ctgtttaatt
     5521 cctgctgcca gcggcgcact tcaaacgggc taagttgctg tgtggccagt gatttgatca
     5581 ccatgccgtg agtgacgtgg ttaatcaggt ctttatccag cggccaggag agaaacagat
     5641 gcatcggcag attaaaaatc gccatgctct gacaggttcc ggtatctgtt agttggtgcg
     5701 gtgtacaggc ccagaacagc gtgatattac cctgattgat attcactttt tcattgttga
     5761 tcaggtattc cacatcgcca tcgaaaggca cattcacttc gacttgacca tgccagtggc
     5821 tggtgggcat gatatgcggt gcgcgaaact caatctccat ccgctggtat tccgaataca
     5881 gcgacagcgg gctgcgggtc tgtttttcgt cgctgctgca cataaacgta tctgtattca
     5941 tggatggctc tctttcctgg aatatcagaa ttatggcagg agtgagggag gatgactgcg
     6001 agtgggagca cggttttcac cctcttccca gagaggcgag gggagcgtcc gagcatcatg
     6061 aggccgaaaa ctctgctttt caggtaactt attaccataa actcagattt actgctgctt
     6121 cacgcaggat ctgagtttat gggaatgctc aacctggaag ccggaggttt tctgcagatt
     6181 cgcctgccat gatgaagtta ttcaagcaag ccaggagatc tgcatgatga ctgcacccaa
     6241 aattacattt atcggcgctg gttcgacgat tttcgttaaa aatattcttg gtgatgtgtt
     6301 ccatcgcgag gcgctgaaaa cggcgcatat tgccctgatg gacattgatc ccacccgcct
     6361 ggaagagtcg catattgtgg tgcgtaagct gatggattca gcaggggcca gcggcaaaat
     6421 cacctgccac acccaacaga aagaagcctt acaggatgcc gattttgtcg tggtggcatt
     6481 tcagattggc ggttatgaac cttgcacggt gactgatttc gaggtctgta agcggcatgg
     6541 tctggaacaa accattgccg atacgttggg gccgggcggt attatgcgcg cgctacgtac
     6601 cattccgcat ctgtggcaaa tttgcgagga catgacggaa gtctgccccg atgccaccat
     6661 gctcaactac gttaacccaa tggcgatgaa tacctgggcg atgtatgccc gctatccgca
     6721 tatcaaacag gtagggctgt gccattcggt gcagggaacg gcggaagagc tggcgcgtga
     6781 cctcaatatc gatccgtcca cgctgcgtta ccgttgcgct ggtatcaacc atatggcgtt
     6841 ttacctggaa ctggagcgta aaaccgccga cggcagttac gtgaatctct acccggaact
     6901 gctggcggct tatgaagcag ggcaggcacc gaagccaaat attcatggta atactcgctg
     6961 ccagaatatt gtgcgctacg aaatgttcaa aaagctgggc tacttcgtta cggaatcgtc
     7021 agaacatttt gctgaataca cgccgtggtt tattaagcca ggtcgtgagg atttgattga
     7081 gcgttataaa gtaccgctgg atgagtaccc gaaacgctgc gtcgagcagc tggcgaactg
     7141 gcataaagag ctggaggagt ataaaaacgc ctcccggatt gatattaaac cgtcacgaga
     7201 atatgccagc acaatcatga acgctatctg gactggcgag ccgagtgtga tttacggcaa
     7261 cgtccgtaac gatggtttga ttgataacct gccacaagga tgttgcgtgg aagtagcctg
     7321 tctggttgat gctaatggca ttcagccgac taaagtcggt acgctacctt cgcatctggc
     7381 cgccctgatg caaaccaaca tcaatgtaca gacgctgctg accgaagcta ttcttacgga
     7441 aaatcgcgac cgtgtttacc acgccgcgat gatggacccg catactgccg ccgtgctggg
     7501 cattgacgaa atatatgctc ttgttgacga cctgattgcc gcccacggcg actggctgcc
     7561 aggctggttg caccgttaaa acgcgactaa acgctactgc gccgggggat ttattccggc
     7621 gcacacctct gacgatacca ataacagaag gcgggcgttg gtaacagcga cccgataccc
     7681 tatgagcatt tcaatgacta caaaactcag ttatggattt ggagcgttcg ggaaggattt
     7741 tgcgatcggc attgtgtata tgtacctcat gtattactac accgatgtcg ttgggctgtc
     7801 tgtgggtttg gtcggtactt tgtttctggt ggcgaggatc tgggatgcta ttaacgatcc
     7861 gattatggga tggattgtaa atgctacgcg atcgcgatga ggtaagttca aaccctggat
     7921 cctgatcggt acgttggcaa actctgtaat cttatttctc ctctttagtg cgcatctgtt
     7981 tgaaggtact actcagattg tctttgtttg cgtgacctac atcctctggg gcatgactta
     8041 caccattatg gatattccct tctggtcgct ggttccaacc atcacgctcg ataaacgtga
     8101 gcgtgaacaa ctggttcctt atccgcgttt ttttgccagt ctggcgggct ttgttacggc
     8161 aggtgtgacg ctaccatttg ttaattatgt cggcggtggc gatcggggat ttggctttca
     8221 gatgttcaca ctggtactga tcgccttttt tattgtttca accatcatca ctctgcgcaa
     8281 tgtgcatgaa gtcttttcgt cagacaatca accgtctgct gaaggaagcc atctgacact
     8341 taaagccatc gttgcgctaa tttataaaaa cgatcagctt tcatgcctct tgggtatggc
     8401 tcttgcttat aatgtagcca gcaacattat taccggattt gctatctatt atttctcata
     8461 tgttatcggt gatgcggatt tgttccccta ttatctgtcg tatgcgggag ctgctaacct
     8521 ggtgacgtta gta
//
LOCUS       DAZPDU010000175         8521 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZPDU010000175 DAZPDU010000000
VERSION     DAZPDU010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8521
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.177"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            134..1456
                     /gene="dcuC"
                     /locus_tag="ACHURS_003670"
     CDS             134..1456
                     /gene="dcuC"
                     /locus_tag="ACHURS_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFR1239302.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVKRTAIPMAVGFVVNMIATITLFY"
     gene            complement(1499..1996)
                     /gene="sspB"
                     /locus_tag="ACHURS_003671"
     CDS             complement(1499..1996)
                     /gene="sspB"
                     /locus_tag="ACHURS_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFR1239303.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(2002..2640)
                     /gene="sspA"
                     /locus_tag="ACHURS_003672"
     CDS             complement(2002..2640)
                     /gene="sspA"
                     /locus_tag="ACHURS_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFR1239304.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIELSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(3035..3427)
                     /gene="rpsI"
                     /locus_tag="ACHURS_003673"
     CDS             complement(3035..3427)
                     /gene="rpsI"
                     /locus_tag="ACHURS_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFR1239305.1"
                     /translation="MAENQYYGTGRRKNSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(3443..3871)
                     /gene="rplM"
                     /locus_tag="ACHURS_003674"
     CDS             complement(3443..3871)
                     /gene="rplM"
                     /locus_tag="ACHURS_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFR1239306.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQVSFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(4090..5217)
                     /gene="zapE"
                     /locus_tag="ACHURS_003675"
     CDS             complement(4090..5217)
                     /gene="zapE"
                     /locus_tag="ACHURS_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFR1239307.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGGKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            5411..5809
                     /gene="zapG"
                     /locus_tag="ACHURS_003676"
     CDS             5411..5809
                     /gene="zapG"
                     /locus_tag="ACHURS_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFR1239308.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            5963..7330
                     /gene="degQ"
                     /locus_tag="ACHURS_003677"
     CDS             5963..7330
                     /gene="degQ"
                     /locus_tag="ACHURS_003677"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFR1239309.1"
                     /translation="MKKQPQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPEQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMIAPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKSAIIALQIVRGNESI
                     YLLMR"
     gene            7420..8487
                     /gene="degS"
                     /locus_tag="ACHURS_003678"
     CDS             7420..8487
                     /gene="degS"
                     /locus_tag="ACHURS_003678"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFR1239310.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
BASE COUNT         2064 a         2161 c         2185 g         2111 t
ORIGIN      
        1 atgaaaaatc acatcaccta caattaaaaa cacgacatcc gcaccataaa tagacttgca
       61 aaaaatataa catcattgtt ttcaatctgc cgtttatggg attgaccgtt ttcttttgac
      121 acggagttca acaatgttcg gcataattat atctgtcatc gtattaatta cgatgggcta
      181 tttgatcctg aaaaactaca aacctcaggt ggtgctggct gccgcaggtg tcttcctgat
      241 gatgtgcgga gtctggttag ggttcggtgg tgtactcgat cccgccaaaa gcagcggcta
      301 cttgatcgtc gacatttata atgaaatcct gcgcatgctg tccaaccgca ttgccggatt
      361 ggggctgtcg attatggcgg tgggcggtta tgcccgctac atggagcgca taggagccag
      421 tcgcgcgatg gtgagcttgc taagccgccc gttaaaactc attcgctcgc cgtatattat
      481 tctgtcggca acttacgtca tcggccaaat catggcgcag tttattacca gcgcctccgg
      541 tctgggtatg ttgctgatgg tcaccttatt tccgacgctg gtgagtctgg gagtaagtcg
      601 cctctctgcg gtggcggtta tcgcaaccac gatgtccatt gagtggggga ttctggaaac
      661 gaactccatt tttgcagccc aggtcgcggg aatgaaaatt gccacatact tcttccacta
      721 ccagcttccg gtcgcctctt gcgtcattat ctcggtggcg atctcccact ttttcgtgca
      781 acgcgctttt gacaaaaaag ataaaaatat caatcacgaa caggcagagc aaaaaactct
      841 cgataatgtc ccgccgctct attacgccat tttacctgtg atgccgttaa tcctgatgct
      901 cggctcgctg ttcctcgccc acgtcgggct gatgaagtca gaactgcatc tggtggtggt
      961 gatgttacta agtttgacgg tgacgatgtt tgttgagttc ttccgcaagc ataacttgcg
     1021 cgaaacaatg gacgatgtgc aggcgttttt tgacggcatg ggtacgcagt ttgccaacgt
     1081 ggtaacgctg gtggtcgcgg gtgaaatatt tgcgaaaggc ttaacgacga ttggcactgt
     1141 tgatgcggtt atcaggggtg cggagcattc tggtctgggc ggtattggcg tgatgattat
     1201 tatggcgcta gtcattgcca tttgtgccat tgtgatgggc tctggcaatg cgccgtttat
     1261 gtcatttgcc agtcttattc cgaatatcgc agccggacta catgtaccag cggttgtaat
     1321 gattatgccg atgcattttg ccacgacgct agcgcgcgcg gtttcgccga ttactgcggt
     1381 ggtgaagcgg acagcgatcc ccatggcagt cggtttcgtg gtgaatatga ttgccacaat
     1441 cacgctattt tattaagtca ttaaaaagac aaaacaggcc gcctgggcct gttttgtatt
     1501 acttcacaac acgtaatgcc ggtcgaccac cgcgtggtgg ctgcggaggt tcatcgtcag
     1561 gatgagtgtc atcatcgtga tctggcttgt cgccatcaat aaccgacata acggtttcgt
     1621 tgtctgccga tgcctcttca tcattcatga tgctggtatc ttcatcgtag gcagcttcag
     1681 gctcaaacat cgtgcctgcg ccattttcac gggcgtagat agccagcacg gcagccagcg
     1741 gcacagaaac ctgacgcgga atgccaccaa agcgcgcgtt aaagcgcacc tcatcattcg
     1801 ccagttccag attgccgaca gcacgcggcg caatgttgag tacgatttgc ccgtcacgcg
     1861 catattccat aggaacctgc acgccaggga gcgtcacatc caccaccagg tgcggcgtga
     1921 gctggttatc cagcaaccac tcatagaatg cacgcagcag atagggacga cgtggtgtta
     1981 gctgtgacaa atccatacag attaactccg gcccagacgc atttcacgtt ctgcttcagt
     2041 taaagaagca aggaaagagt cacgctcaaa gacgcgggtc atatagcctt tcagctcttt
     2101 cgcacccggg ccgctgagct cgatacccag ttgcggcaga cgccacagca gcggagcaag
     2161 atagcaatcg accaggctga actcatcgct caggaagtac ggcttctgac cgaagaccgg
     2221 cgcaatcgcc agcagttctt cgcgcagttg cttacgtgcg gcatctgctt cagaagctga
     2281 accgttgatg atggtgttca tcagcgtgta ccagtctttt tcgatgcgat gcatgtacag
     2341 acggctttca ccgcgagcta ccgggtaaac aggcatcagt ggcggatgcg ggaaacgctc
     2401 atccagatat tccataatga tgcgagattc ccacagggtc agctcacgat ccaccagggt
     2461 cggaacgctc tgattcgggt tgaggtcaat cagatcctga ggcggattgt ccttttccac
     2521 gtgttcgatc tcgaaactta cacctttctc agccagcaca atgcggacct gatggctata
     2581 gatgtcagta ggaccggaaa acagcgtcat taccgaacgt ttgttggcag cgacagccat
     2641 gaaaacctcc aggtatagtc agaattttta ctgctaccag ccaccaggtg gccagtcaga
     2701 agttgtgtta cccaataagg aacgactctc tttgttcgaa aatcaaacaa aaaatgagca
     2761 atacccgaca tttgggcaga aaattggatg atagtttacc agattttgcg accattgtgg
     2821 tgagtggatg ccggaaatgg ggaaaaagag atgcgcttta gtctgaaata gttgacttag
     2881 acccttattg gcgatgtggt ttttgttttg cctgtctttc agacggcagc aaaaagcaac
     2941 ttcccatttt ttacgctgat tcagaattta gccataaaaa aacccgccga agcgggtttt
     3001 ttcgtaaatt gttttctgcc ggagcagaag ccaattaacg tttggagaac tgcggacgac
     3061 gacgtgcttt acgcagaccg actttcttac gttcaacctg acgagcgtca cgagtaacga
     3121 agccagcttt acgcagttca gaacgcaggg actcgtcgta ttccatcaga gcgcgggtga
     3181 taccgtgacg gatcgcacca gcctgaccag agataccacc acctttaacg gtgatgtaca
     3241 ggtccagttt ctcaaccatg tcgaccagtt ccagcggctg acgaactacc atgcgggcag
     3301 tttcacgacc gaagtactgt tccagagaac gttggttgat tacgatttta ccgttgcccg
     3361 gtttgatgaa aacgcgagct gcggaatttt tgcggcgacc agtgccgtag tattgatttt
     3421 cagccattgc ctataatccc gattagatgt caagaacttg cggttgctgt gccgcgtggt
     3481 tgtgctcgtt acccgcgtaa actttcagtt tacggaacat agcacgaccc agcgggcctt
     3541 ttggcaacat gcctttaacc gcgatttcaa tcacacgctc aggacggcga gcaatcatct
     3601 cttcaaagct cacttgtttg ataccaccga tgtggccggt gtggtgatag tacactttgt
     3661 cagtacgctt gttgccggtt acagcaactt tgtcagcgtt cagaacgatg atgtaatcac
     3721 cggtatctac gtgcggagtg tattccgctt tgtgcttacc gcgtaggcga cgagccagtt
     3781 cagtagccag acggcccaga gttttaccgg tcgcgtcaac aacataccag tcgcgtttta
     3841 cggtttctgg tttagctgta aaagttttca ttaaaagctt acccaataaa tagttacacg
     3901 ttggtgaaca cccaaacgtc ttcaattgtt gaggttcaca cgacaaagtc cggcaaacct
     3961 accccttcga atagcctatg ccagcacaca aaaagttttg ggaaaaaaac tttcttgtaa
     4021 cgtggggtcg caggattata gagaagtcgg ggtcaaagat cgaccccttt ttgtgatttg
     4081 tgacaggttt taacccgcca aatgctcgcg cttcagatac tcttcgcttt gcatctcttg
     4141 cagacgtgac aggcaacgct ggaactcaaa cttcagccga tcgccctgat aaatttcata
     4201 cagcggcact tctgcactca ccactaattt gacatggcgc tcgtaaaact catccaccag
     4261 cgcaataaag cgccgcgctt cgctctccat caaccgcgtc ataactggta catcaaacaa
     4321 catgaccgta tgaaagagac gtgagagcgc aatatagtca tgctgactgc gggcgtcgac
     4381 gcacagcgta gtaaaagaga ccgccagcgt ctggttctcg acgcccattg tcgctaatgg
     4441 ccgatggttg atttctaacg tcggtgaatt ttctcgtttc ccccccgcca gcgccaacca
     4501 tagtttatcc atttgcgccc gggtttcatc gtggagtggc gaaagccaca gatgcgcctg
     4561 agtgagtgta cgcagtcgat aatcaacacc agcgtccacg ttcattacat cacaatgctg
     4621 tttaatggca tcgattgcag gcagaaaacg cgcacgttgc aagccatttc gataaagttc
     4681 gtccggcgga atatttgacg tcgctaccag ggtaataccg cgggcgaaca aggctttcat
     4741 cagaccgcca agtagcatgg catcggtaat atcagaaaca aaaaattcgt caaaacagag
     4801 cacgtcagtt tcggctttaa agcgatcggc aataatttcc agcggatcgg tctgcccctg
     4861 taaggcagtt agctcttcat gcacccgcag cataaaacgg tgaaagtgca ggcgctgttt
     4921 ccgctctccc ggcaggcttt gatagaaaag gtccatcagc caggttttcc cgcgtcctac
     4981 accgccccac atatataagc cacgcactgg cgtatgcttt gtgtcttcgc gtttacccca
     5041 cagcttaccg acccgcgcca ttagcccact cgtcctgggg gctggtggcg tgctattgat
     5101 gagttcctga taaataattt ccaggcggct gacggcctct ttttgaacgt cgtcgggttg
     5161 atggctgcct tcattgagcg ccttcaggta ttgcgatgtt ggggtaacgc tttgcatgat
     5221 cttattgtta ttccttgaat aatcggtgcg ccgttgttca cggttgacga aaaaaaggcc
     5281 gttctacact acgcgatatg cagtcgggat tccacttctg tggaattaac ggttatagtg
     5341 gcataatcag ccgcaggcat ggagcctgaa gccaacaccc tacggaaaca aaagacaacg
     5401 ggagatgttc atgacctggg aatatgcgct aattgggtta gtcgtcggca tcattattgg
     5461 tgctgtggcc atgcgttttg gtaatcgtaa actacgccag caacaggcgt tgcagtacga
     5521 actggaaaag aataaagctg aactggacga gtatcgcgaa gagctggtta gccactttgc
     5581 ccgcagcgcg gaattactgg ataccatggc gcacgactat cgccagctgt atcagcacat
     5641 ggcgaaaagc tccagcagcc tgctgccgga actgtctgct gaagcaaacc cgttccgtaa
     5701 tcgtctggca gagtctgaag ccagcaacga tcaggcaccg gtgcagatgc ctcgcgacta
     5761 ttctgaaggc gcatccggcc tgctgcgtac tggcgcgaag cgcgactaat ttatttttcg
     5821 ggcgcagcca ttgcgccctc ctcttctctc cctccccgac tatcatttaa tctggtgtct
     5881 cattgttagc cgtctgaaaa ttcaataaca tcaaactgtt ttgaatctct tttcttatca
     5941 ttcaggtacg agagcaggaa taatgaaaaa acaaccccag ctgttgagtg cattagcgtt
     6001 aagtgtcggg ttaactctct cggcgtcatt tcaggccgtc gcgtcgattc caggccaggt
     6061 tgccgatcag gcccctctcc ccagtctggc tccaatgctg gaaaaagtgc ttccggcagt
     6121 ggtgagcgta cgggtggaag gaacggccag tcagggacag aaaatcccgg aagaattcaa
     6181 aaagtttttt ggtgatgatt taccggagca acctgcacaa cccttcgaag gtttaggctc
     6241 cggtgtcatc atcaacgcca gtaaaggcta tgtgctgacc aacaaccatg tgattaatca
     6301 ggcacagaaa atcagtattc agctcaatga tgggcgcgag tttgatgcaa aactgattgg
     6361 tagcgatgac cagagcgata tcgccctgtt acaaattcaa aacccgagca aattaacgca
     6421 aatcgctatt gccgactccg ataaattgcg cgtcggtgat tttgccgtag cggtcggtaa
     6481 cccatttggc cttgggcaaa ccgccacctc tggcattgtt tccgcattag gacgcagcgg
     6541 gttgaatctt gaaggtctgg aaaactttat ccagacagat gcttccatta accgcggtaa
     6601 ctccggcggt gcactgttaa accttaacgg tgagttaatt ggcatcaaca ctgcaatcct
     6661 tgcgcctggc ggcgggagcg tcgggattgg atttgccatc cccagtaata tggcgcgaac
     6721 actggcgcag cagcttatcg actttggtga aatcaaacgc ggtttgttag gcatcaaagg
     6781 caccgagatg agtgccgata tcgccaaagc cttcaacctt gacgtgcagc gtggcgcgtt
     6841 tgtcagcgaa gtgttgccag gttctggctc ggcgaaagcg ggcgtcaaag cgggcgatat
     6901 tattaccagc ctcaacggca aaccgctgaa tagctttgct gagttgcgct ctcgtatcgc
     6961 gaccaccgag ccgggcacaa aagtgaaact tggcctgctg cgtaacggca aaccactgga
     7021 agtagaagtg acgctcgata ccagcacctc ttcgtcggcc agcgctgaaa tgatcgcgcc
     7081 agcgctggaa ggtgcaacgt tgagcgatgg tcagctaaaa gatggcggca aaggtattaa
     7141 gatcgatgaa gttgtcaaag gaagcccagc tgctcaggct ggcttgcaaa aagacgatgt
     7201 gatcattggc gtcaaccgcg atcgggtgaa ctcgattgct gaaatgcgta aagtgctggc
     7261 ggcaaaatcg gccatcatcg ccctgcaaat tgtacgcggc aatgaaagca tctatctgct
     7321 gatgcgttaa tgtcgtaaac cgggcatcag gcttacgtgt gatgtccggt taactcatgg
     7381 tatgctgctg ccgttccctt ttttaatgac gcctccatca tgtttgtgaa gctcttacgt
     7441 tccgttgcga ttggattaat tgtcggcgct attctgctgg ttgccatgcc ttcgctgcgc
     7501 agccttaacc cgctttccac tccgcaattt gacagtaccg atgagacgcc tgccagctat
     7561 aatctggcgg ttcgccgcgc cgcgccagcg gtggttaacg tttacaaccg tggtttgaac
     7621 accaactctc acaaccagct tgagatccgc accctgggat ccggtgtaat catggatcaa
     7681 cgcggttata tcatcaccaa taaacacgtc atcaacgacg ccgatcagat catcgtcgcc
     7741 ttacaggatg gtcgtgtatt tgaagcattg ctggtgggat ctgactctct aaccgatctg
     7801 gcggtactta aaattaatgc cactggcggt ttacctacca ttccaatcaa tgcacgtcgc
     7861 gtaccgcata ttggcgacgt ggtactggcg atcggtaacc cgtacaacct cgggcagacc
     7921 attacccagg ggattattag tgccacgggt cgaattggtc tgaacccgac cgggcggcaa
     7981 aacttcctgc aaaccgatgc ttccattaac cacggtaact ctggcggcgc gctggtgaac
     8041 tcgctgggcg aactgatggg cattaacacg ctgtcgtttg ataagagtaa cgatggcgaa
     8101 acgccggaag gtatcggctt tgcgattcct ttccagttag caaccaaaat tatggataag
     8161 ctgatccgcg atggtcgcgt gatccgcggc tacattggca ttggcggacg tgagatcgca
     8221 ccgctgcacg cacagggcgg tggtatagat caactgcaag ggatcgtggt taatgaagtg
     8281 tcacctgacg gcccggcggc gaatgcgggt attcaggtca acgatctgat tatttcggtg
     8341 gataacaaac cggccatctc tgctctggag acgatggatc aggtggcgga aattcgccct
     8401 ggttcggtga tcccggttgt agtgatgcgt gatgataagc agttaacgct gcaggtcacc
     8461 attcaggaat atccggcaac caattaagtc gagtgctcaa aacaaaaaac cggagtctgt
     8521 g
//
LOCUS       DAZPDU010000176         8491 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZPDU010000176 DAZPDU010000000
VERSION     DAZPDU010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8491
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.178"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(124..330)
                     /locus_tag="ACHURS_003679"
     CDS             complement(124..330)
                     /locus_tag="ACHURS_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFR1239311.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            643..732
                     /gene="kdpF"
                     /locus_tag="ACHURS_003680"
     CDS             643..732
                     /gene="kdpF"
                     /locus_tag="ACHURS_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFR1239312.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            732..2405
                     /gene="kdpA"
                     /locus_tag="ACHURS_003681"
     CDS             732..2405
                     /gene="kdpA"
                     /locus_tag="ACHURS_003681"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFR1239313.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLIALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2428..4476
                     /gene="kdpB"
                     /locus_tag="ACHURS_003682"
     CDS             2428..4476
                     /gene="kdpB"
                     /locus_tag="ACHURS_003682"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFR1239314.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHIEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4485..5057
                     /gene="kdpC"
                     /locus_tag="ACHURS_003683"
     CDS             4485..5057
                     /gene="kdpC"
                     /locus_tag="ACHURS_003683"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFR1239315.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPDASASIPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5050..7734
                     /gene="kdpD"
                     /locus_tag="ACHURS_003684"
     CDS             5050..7734
                     /gene="kdpD"
                     /locus_tag="ACHURS_003684"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFR1239316.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRLAYRGRHICEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPSEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHFEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7731..8408
                     /gene="kdpE"
                     /locus_tag="ACHURS_003685"
     CDS             7731..8408
                     /gene="kdpE"
                     /locus_tag="ACHURS_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFR1239317.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
BASE COUNT         1772 a         2205 c         2457 g         2057 t
ORIGIN      
        1 tatttcatga atatatgttt cgattggtat aacggtgtct aaatattcct ctgaacatct
       61 tcctgataca aaaaaccgcc agcgtttccg ctggcggttt tcaatgtatt cagaactcag
      121 caatcagtaa aaatcaccgg ttgccttttc ggcctgatcc atccacaccg gtttatcgct
      181 ggttttcgac cagacgcggt gcaggtagct gtaaaagcgg gcgcggtctt tccagaaaag
      241 catgaaaggc agcgccagaa cgcccgctgc aaccgcatat gtacggcgta aaaagataag
      301 ccatgcagga tattctctgt agagttccat acttttctcc cccttttgta tctacccggt
      361 gaatagcacc ggaaaaatga atttgtttat ctgatgaaaa tagtaccgcc ttttgtgtaa
      421 ttttactact catccgacca cttatttttg cttattgatg gtttatttac attcactctg
      481 taattaagtt acacaaaagt taaattaata ctaaacatta gttaaatcat ggcttttgcc
      541 atttttatac tttttttaca ccccgcccgc agatttttgc gaaatctttg cagccagaat
      601 tctactcttc cggtatcact tttaggccac tggaggtgca ctgtgagtgc aggcgtgata
      661 gccggcgtgt tgctggtgtt tttattactg ggttatttgg tttatgccct gatcaatgcg
      721 gaggcgttct gatggctgcg caagggttct tactgattgc cacgttttta ctggtattaa
      781 tggtgctggc gcgtccttta ggcagcgggc tggcgcggct gattaatgac attcctcttc
      841 caggtacagc gggcgttgaa cgtatactct ttcgtttacc tggcgtctct gaccatgaga
      901 tgaactggaa gcaatatctt tgtgccattc tcggcctgaa catgctgggg ctggcggtgc
      961 tgttttttat gttgctcggt cagcactatc tgccgcttaa tccacagcag ttgccggggc
     1021 tgtcgtggga tctggcgctg aataccgccg tcagctttgt cacgaatacc aactggcaat
     1081 cttatagcgg tgaaaccacg ttgagctatt tcagccagat ggcaggctta acggtacaaa
     1141 actttctttc tgccgccagc gggattgcgg tgatttttgc cttcatccgt gcgtttactc
     1201 gccagagcat gagcacactc gggaatgcct gggtcgatct gctacgcatc acgttatggg
     1261 tgctagtccc tgtggcgttg ttgatcgcac tgttttttat tcaacaaggt gcgctgcaaa
     1321 actttctgcc ctatcaggca gtgaataccg ttgaaggagc gcaacagctg ttacccatgg
     1381 gaccagtagc ttctcaggaa gcgatcaaga tgctcggtac taacggcggt ggcttcttta
     1441 atgccaactc gtcgcatccg tttgaaaacc caaccgcact gaccaacttc gtgcagatgc
     1501 tggcgatctt cttgatccca acagcgctgt gctttgcctt tggtgaagtg acgggcgatc
     1561 gccgccaggg gcgcatgttg ctgtgggcga tgtcagtgat ttttgtcatc tgcgtaggcg
     1621 tggttatgtg ggcagaagtt cagggtaatc ctcatctgct ggcgctcggc gcagacagca
     1681 gcatcaatat ggaaggtaaa gagagccgtt tcggcgtgct ggtcagtagc ctgtttgcgg
     1741 tcgtgactac ggcggcgtcc tgtggcgcgg tgattgcgat gcatgattcg tttaccgctc
     1801 tcggcggcat ggtgccgatg tggctaatgc aaattggtga agtggtgttc ggcggtgtcg
     1861 gttctggtct ttacggcatg atgctgtttg tcctgctggc ggtgtttatt gccgggctga
     1921 tgattggtcg tacaccggaa tatctgggta aaaaaatcga cgtacgcgag atgaaactga
     1981 ttgcactggc gattctggtt actccaacac tggtactgat gggcgcggcg ttggcgatga
     2041 tgaccgacgc cggacgtagc gccatgctta accctggccc gcatggtttt agcgaagtgc
     2101 tgtacgccgt gtcgtccgcc gctaacaaca acggcagcgc ctttgccgga ttaagcgcca
     2161 actctccgtt ctggaactgt ttactggcgt tctgcatgtt tgtcggtcgc ttcggggtga
     2221 ttatcccggt gatggcaatt gccggttcgc tggtgagtaa aaagagccaa cccgccagct
     2281 ccggcacgct gccaacgcac ggcccgctgt ttgttggcct gttaatcggc accgtgttgc
     2341 tggttggcgc actgaccttt atccctgccc tggcgcttgg tccggtggcg gaatatctct
     2401 cctgatgata ttgagcgagc actgaatatg agtcgtaaac aactggcgct attcgaacca
     2461 acacttgtcg ttcaggcgct gaaagaagcg gtgaaaaaat taaacccgca ggcgcaatgg
     2521 cgcaatccgg tgatgtttat cgtctggatc ggcagtctgc tgaccacctg tattagcatc
     2581 gcgatggcaa gcggtgcgat gcctggcaat gcgctgttta gcgcggcaat tagcggctgg
     2641 ctgtgggtca ccgtactgtt cgctaatttc gccgaggcgc tggcagaagg ccgcagtaaa
     2701 gcgcaggcca acagtcttaa aggggtgaaa aaaactgcct ttgcccgcaa gctgcgtgag
     2761 ccgaaatatg gcgctgcggc ggacaaagtt cctgccgatc aactgcgtaa aggtgacatc
     2821 gtgctggtag aagctggcga tattatcccc tgcgatggtg aagtcattga aggcggcgca
     2881 tcggtcgatg aaagcgccat caccggggaa tcggcaccgg tgatccgtga atccggcggc
     2941 gattttgcct ccgtcaccgg cggcacgcgt attctttctg actggctggt gattgagtgt
     3001 agcgttaacc ccggtgagac atttctggat cggatgatcg cgatggtgga aggcgcacag
     3061 cgacgcaaaa caccgaacga gattgccctg actatcctgc tgattgctct gaccatcgtc
     3121 tttttactgg caaccgccac gctgtggccg ttttccgcgt ggggcggtaa tgccgtgagt
     3181 gtaacggtac tggtggcgct gctggtctgt ctgatcccaa ccactattgg cggcctgttg
     3241 tcagcgatcg gcgtcgccgg gatgagtcgg atgctgggcg cgaatgtgat tgccaccagc
     3301 ggacgtgcag ttgaagcggc aggtgacgtt gacgttctgc tactggataa aaccggcacc
     3361 atcacactcg gtaaccgtca ggcgtcggag tttatccccg cgcagggcgt ggatgaaaaa
     3421 acgctggctg acgccgcaca actggcttcg ctggctgatg aaacgccgga aggtcgcagt
     3481 attgtgatcc tcgccaagca acgttttaac ctgcgcgagc gcgatgtgca gtcgctccat
     3541 gccacctttg taccgtttac tgcgcaaagc cggatgagcg ggatcaacat cgacaaccgc
     3601 atgatccgta aaggttctgt cgatgccatt cgccgccata ttgaggctaa cggcggtcac
     3661 ttccctgccg atgttgatca aaaagtcgat caggttgcgc gtcagggagc cacgccgctg
     3721 gtggtggtgg aaggttctcg tgtgctgggc gttattgcgc tgaaagatat cgtcaaaggc
     3781 ggtattaaag agcgcttcgc ccagctgcgc aaaatgggca ttaaaacggt gatgattacc
     3841 ggcgataacc gtctgactgc cgccgcgata gctgcggaag cgggtgtcga tgattttctc
     3901 gccgaagcaa caccggaggc caagctggca ttgatccgtc agtatcaggc ggaaggtcgt
     3961 ttggtagcga tgaccggcga cggcaccaac gatgctccgg cgctggcgca ggcagatgtc
     4021 gcggtggcga tgaactccgg cacccaggcg gcgaaagagg cgggcaatat ggtggatctc
     4081 gactctaacc cgaccaagtt gatcgaggtg gtgcacattg gcaaacagat gctgatgacc
     4141 cgtggctcgc tgaccacctt cagcattgcc aacgatgtgg cgaaatactt cgccattatt
     4201 ccggcggcgt tcgcggcaac atatccgcag ttaaatgcgc tgaacatcat gcgcctgcat
     4261 tcgcccgact ccgccattct cagtgcggtc attttcaacg ccttgattat cgtctttttg
     4321 attcctctgg cgttaaaagg cgtgagttat aaaccgctta ccgcttctgc catgttgcgc
     4381 cgtaacttat ggatttacgg tctgggtggg ctgctggtgc cgtttattgg tatcaaagtc
     4441 attgatttac tgctgaccgt ttgcggtctg gtgtgaggtt taccatgagt ggattacgtc
     4501 cggcattatc gacatttctc tttctgttat tgattactgg cggcgtttac ccgctgctga
     4561 ccaccgcact ggggcaatgg tggtttccct ggcaggccaa cggttcgttg atccgtgaag
     4621 gtgatacggt gcgtggctcg gcattaatcg ggcagaattt taccggcaac ggctattttc
     4681 atggtcgccc gtcagcaacg gctgaaatgc cctataatcc acaggcttct ggcgggagca
     4741 atctggcggt cagtaaccct gagctggata aactaatagc cgcacgcgtt gctgcattac
     4801 gggccgctaa cccggatgcc agcgcgagca ttccggttga actggtgacg gcatcggcaa
     4861 gcgggctgga caataatatc accccgcaag cggcggcctg gcagatccca cgcatcgcga
     4921 aagcgcgtaa tctcagcgtt gaacagctca cgcagctgat cgcaaaatac agccaacaac
     4981 cgctggtgaa atatatcggc cagccggtcg tcaacattgt cgaactcaat ctggcgctgg
     5041 ataagcttga tgaataacga acccttacgt cccgaccccg atcgtctgct ggaacaaact
     5101 gccgcgccgc atcgggggaa gctgaaagtt ttcttcggtg cctgtgcagg cgtcgggaag
     5161 acctgggcga tgctggcaga agcccagcga ctgcgggcgc aagggctgga tattgtggtt
     5221 ggcgtggtag aaacccacgg gcgaaaagat accgccgcaa tgctggaagg gctggctgtt
     5281 ctgccgccaa aacgcctggc gtaccgtggg cggcacatct gcgagtttga cctcgatgcc
     5341 gccctcgccc gccgtccggc gctgatctta atggacgaac tggcgcacag taatgcgcca
     5401 ggttctcgtc accctaaacg ctggcaggac atcgaagagc tgctggaagc aggcattgat
     5461 gttttcacta ccgtcaacgt tcagcatctg gaaagtctga atgatgtggt cagcggcgtc
     5521 accggaattc aggtacggga aaccgtgccc gatccttttt tcgatgccgc cgacgacgtg
     5581 gttctggtgg acttgccccc ggacgatctg cgccagcggc tgaaagaagg caaagtctat
     5641 attgccgggc aggcggagcg cgccattgaa cattttttcc gcaaaggcaa tctgatcgcc
     5701 ctgcgcgaac tggcactgcg ccgtactgcc gatcgcgttg atgagcaaat gcgcgcctgg
     5761 cgggggcatc ctagcgaaga gaaagtctgg cacacgcgcg acgcgatcct tttatgcatc
     5821 ggccataaca ccggtagtga gaagctggtc cgcgcggcgg cgcggctggc gtcacggctg
     5881 ggtagcgtct ggcacgcggt gtatgttgaa acccctgccc tgcatcgctt accggagaaa
     5941 aaacgtcgtg caattctcag cgccttacgt ctggcgcagg aactgggcgc ggagacggca
     6001 acactttctg atccagcgga agagaaagcg gtagtgcgtt atgcccgtga acataatctc
     6061 ggcaagatta ttctcggtcg cccggcctcg cgccgctggt ggcgtcggga aacgtttgct
     6121 gaccgactgg cgcgcatcgc ccccgatctc gatcaggtgc tggtcgcgct tgatgaacca
     6181 cccgcccgca cgattaacaa cgcgccggat agccgctctt ttaaagacaa gtggcgggta
     6241 cagattcagg gatgcgtggt tgccgccgcg ttatgcgccg ttatcacctt aattgccatg
     6301 cagtggctga tggcgtttga tgccgccaac ctggtgatgc tgtatctgct tggcgtggtg
     6361 gtggtggcgc tattttatgg acgctggcct tcggtggttg ccaccgtcat taatgtggtg
     6421 agtttcgatc tcttttttat cgccccacgc ggcacgctcg ccgtctctga tgtgcaatat
     6481 ctgctgacct tcgcggtgat gttaaccgtc gggctggtga tcgggaacct tactgctggc
     6541 gtgcgttatc aggcgcgggt agcccgttac cgcgagcaac gcacacggca cttatatgaa
     6601 atgtcgaaag ctctggcggt gggccgcagt ccgcaggaca tcgccgccac cagcgaacaa
     6661 tttattgcct ccacgtttca tgcccgcagt caggtgttgt tgcccgatga caacggtaaa
     6721 ttgcagccgt taacacatcc gcaaggaatg acgccatggg acgatgccat cgcgcagtgg
     6781 agttttgata aaggcctgcc tgcgggcgcg ggcaccgaca cgttacccgg tgtaccgtac
     6841 cagattttgc cgctaaaaag cggcgagaaa acctacgggc tggtggtggt ggagccgggg
     6901 aatctgcgcc agttgatgat cccggaacag cagcgcctgc tggagacgtt tacgctgtta
     6961 gtcgccaatg cccttgagcg gctgacgcta accgccagcg aagaacaggc gcggatggca
     7021 agcgaacgtg aacagatccg caacgccctg ctggcagcgc tttcgcatga tttacgcacg
     7081 ccgcttacgg tgctgtttgg tcaggcagaa atcttaacgc tcgatctggc aagcgaagga
     7141 tcaccccacg cccgccaggc cagcgagatt cgtcagcatg tgctgaacac tacccgactg
     7201 gtgaataatc tactggatat ggcgcgaatt cagtccggcg gctttaattt gaagaaagag
     7261 tggttaacgc tggaagaagt agtcggcagc gcgctgcaaa tgctggaacc gggtttatcg
     7321 tcgcccatca atctttctct gccagaaccg ctgaccttaa tccacgttga cgggccactc
     7381 tttgaacggg tgctgattaa tctgctggag aacgcggtga aatatgcggg tgcgcaggcc
     7441 gaaattggta tcgatgccca ctttgagggc gaaaatctac aactggatgt ctgggataac
     7501 ggccccggtc ttccgccagg ccaggagcag acgatatttg ataagtttgc tcgcgggaat
     7561 aaagagtcgg cggtgccggg tgtcgggctt gggttggcaa tttgtcgggc gattgtggat
     7621 gtacacggcg gcaccattac cgcgttcaac cgaccggaag gtggtgcctg ttttcgtgtt
     7681 acacttcccc agcaaaccgc tcctgaactt gaagactttc atgaggatat gtgacaaacg
     7741 ttctgattgt tgaagatgaa caggctattc gtcgctttct gcgcacggcg ctggagggcg
     7801 acgggatgcg cgtctttgag gccgaaactc tgcaacgcgg cttgctggaa gcggcaaccc
     7861 gtaagccaga tttgattatt ctcgatctcg gcctgcccga tggtgatggg attgagttta
     7921 tccgcgacct gcgccagtgg agcccggtgc cggtgattgt gctttccgca cgcagcgaag
     7981 agagcgacaa gatcgccgcg ctggatgccg gagcggatga ttatctgagt aagccgtttg
     8041 gcattggcga attgcaggcc cgtctgcgcg tcgctttacg ccgccactcc gccaccgccg
     8101 cgcccgatcc gctggtgaaa ttttccgatg ttaccgtcga tttagccgcc cgcgtgattc
     8161 accggggtga ggaagaggtg catctcacac caattgagtt ccgcctgctg gcggtgctgc
     8221 tcaacaatgc cggaaaagta ctcacccagc gccagctcct taaccaggtg tgggggccaa
     8281 acgcggtcga acacagtcac tatttgcgta tttatatggg acatctgcga caaaaactgg
     8341 aacaggatcc ggcccggcct cgccacttca ttactgaaac cggtattgga tatcggttta
     8401 tgctttgaat tgttattcaa taaattcgct taaatagtga ttaatttatt attaaacctg
     8461 cgccgaatat tattatttgg caggtttaat t
//
LOCUS       DAZPDU010000177         8395 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZPDU010000177 DAZPDU010000000
VERSION     DAZPDU010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8395
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.179"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(132..208)
                     /locus_tag="ACHURS_003686"
     tRNA            complement(132..208)
                     /locus_tag="ACHURS_003686"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(172..174),aa:Asp,seq:gtc)
     gene            complement(341..1072)
                     /gene="dnaQ"
                     /locus_tag="ACHURS_003687"
     CDS             complement(341..1072)
                     /gene="dnaQ"
                     /locus_tag="ACHURS_003687"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFR1239318.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            1137..1604
                     /gene="rnhA"
                     /locus_tag="ACHURS_003688"
     CDS             1137..1604
                     /gene="rnhA"
                     /locus_tag="ACHURS_003688"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFR1239319.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1601..2323)
                     /locus_tag="ACHURS_003689"
     CDS             complement(1601..2323)
                     /locus_tag="ACHURS_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR1239320.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2357..3112
                     /gene="gloB"
                     /locus_tag="ACHURS_003690"
     CDS             2357..3112
                     /gene="gloB"
                     /locus_tag="ACHURS_003690"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFR1239321.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            3184..4542
                     /gene="mltD"
                     /locus_tag="ACHURS_003691"
     CDS             3184..4542
                     /gene="mltD"
                     /locus_tag="ACHURS_003691"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFR1239322.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4591..5214)
                     /locus_tag="ACHURS_003692"
     CDS             complement(4591..5214)
                     /locus_tag="ACHURS_003692"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR1239323.1"
                     /translation="MSGLPQGRPTFGAAQNVSAVVAYDLSAHMLDVVAQAAEARQLKN
                     ITTRQGYAASLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSP
                     GHPVRDIWLQAVEALRDTSHVRNYASGAWLTLINEANLIVDNLITDKLPLEFSSWVVR
                     MRTPEALVDAIRIYQQSASTEVRTYFSLQTDGSFTSDIIMVEAHKAA"
     gene            complement(5218..6018)
                     /locus_tag="ACHURS_003693"
     CDS             complement(5218..6018)
                     /locus_tag="ACHURS_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFR1239324.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            6259..7173
                     /gene="yafC"
                     /locus_tag="ACHURS_003694"
     CDS             6259..7173
                     /gene="yafC"
                     /locus_tag="ACHURS_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFR1239325.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(7170..7973)
                     /gene="dkgB"
                     /locus_tag="ACHURS_003695"
     CDS             complement(7170..7973)
                     /gene="dkgB"
                     /locus_tag="ACHURS_003695"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFR1239326.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(8136..8212)
                     /locus_tag="ACHURS_003696"
     tRNA            complement(8136..8212)
                     /locus_tag="ACHURS_003696"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8176..8178),aa:Asp,seq:gtc)
BASE COUNT         2241 a         2025 c         2026 g         2103 t
ORIGIN      
        1 tctaccatat gtgattaaca catttacagc ataacaaaac catagaccga agaaatagcg
       61 ggatttaaat gcaaaaaaac cgcaaaatta aaaatcttgc ggttctctga actcattttc
      121 atgagtgaat ttggcggaac ggacgggact cgaacccgcg cccccctgcg tgacaggcag
      181 gtattctaac cgactgaact accgctccgc gttgtgttcc gttgggaacg aggcgaatat
      241 tacggattgc ctcacccttc gtcaacggtt tttctcatct tttgaatcgt ttgctgcaaa
      301 aatcgcccaa gccgctattt ttagcgcctt ttacaggtat ttatgcccgc cagaggcagc
      361 ttccgccctt cttctccacc agatcaagac gggcttcatg agctgcaagc tcttcatctg
      421 tcgcaaaaac aacgcgtaac ttacttgcct gacgtacaat gcgctgaatt gttgcttcac
      481 cttgttgctg ctgtgtctct ccttccatcg caaaagccat cgacgtttga ccaccggtca
      541 tcgccagata aacttccgca aggatctgtg catcgagtaa tgccccgtgt agcgttcgtt
      601 tactgttatc tatttcgtag cgggcacata acgcatcgag gctgttgcgc ttaccaggaa
      661 acattttcct cgccaccgca aggctatcgg tgaccttaca gaaggtattg gtcttcggaa
      721 tatcgcgctt aagcaacgaa aactcgtagt ccataaagcc gatatcgaac gctgcgttat
      781 ggatcaccaa ctccgcgccg cggatatagt ccatgaactc atcggctact tcggcaaacg
      841 tgggcttatc gagcaaaaat tcatcggcaa taccatgtac gccaaaggct tccggatcca
      901 ccagccgatc gggtttgaga taaacatgga agttattgcc cgtcaggcga cggttcacca
      961 cttcaacggc accaatctca atgatcttgt ggccttcata gtgcgcacca atctggttca
     1021 taccggtggt ttcggtatcg agaacgatct ggcgtgtaat tgcagtgctc atagcggtca
     1081 tttatgtcag acttatcgtt ttacagttcg attcaattac aggaagtcta ccagagatgc
     1141 ttaaacaggt agaaattttc accgatggtt cgtgtctggg caatccagga cctgggggtt
     1201 acggcgctat tttacgctat cgcggacgcg agaaaacctt tagcgcaggc tacacccgca
     1261 ccaccaacaa ccgtatggag ttgatggccg ctattgtcgc gctggaggcg ttaaaagaac
     1321 attgcgaagt cattttgagt accgacagcc agtatgtccg ccagggtatc acccagtgga
     1381 tccataactg gaaaaaacgt ggctggaaaa ccgcagacaa aaaaccagta aaaaatgtcg
     1441 atctctggca acgtcttgat gctgccctag ggcaacatca aatcaaatgg gaatgggtta
     1501 aaggccatgc cggacacccg gaaaacgaac gctgtgatga actggctcgt gctgcggcaa
     1561 tgaatcccac actggaagat acgggctacc aagtcgaagt ttaagcttgt ggtttacgac
     1621 attgccgggt ggctccaacc gcctggcgaa ttcgtggctt gtttttactc tgtttcatcg
     1681 ggtttagcgt taaaggaata gtccgtttcc gggcaacaat aagttgtaag caaccaagcg
     1741 caggaatatg cgcattcaat agttttcctc cgtgtttgtt ccacgggaga acgtggaaac
     1801 ggctggcgtg cagcacttca aaattcaaca aagagagcca gtccagttgc cgcatcagag
     1861 taaacatccg gctgttatag ggcgaggttt ttcgcaatac cggcacaagt ttgcgtaatc
     1921 ccattaaact aatgggattg aagccactaa tcaccagcca gccatcatca atcaataccc
     1981 gatcggtttc acgcaataaa cgatgcggat cggtgcacca cggcaatgta tgtgccagta
     2041 gacaaacatc aacggattta tcggcaaaag gaagatgaag tggatccgcc tgcacctgga
     2101 cgggcattcc ttgcgcagaa acattcactt gatgagaaac cgcgcacgct tcgcaattga
     2161 tttctgcgct taaattgcca atcttaagca gatgaaaacc atacatttta gtgaaccacg
     2221 ggttgagctg gcgctccagc gccttgcgat aaagctctcc ccagggcaaa tcgccccagc
     2281 gatcaggagc cacgacagtt tgagggactc ttgccggttt catcacaacc ttccgtttca
     2341 cactaagagg taatctatga atcttaacag tattcccgcc tttgatgaca attacatctg
     2401 ggttttgaat gatgaagcag gtcgctgcct gattgtcgat cccggagacg cagagccagt
     2461 attaaacgcc attgccgcca ataactggca accggaggcc atatttctca cccaccatca
     2521 tcacgatcac gttggcggcg taaaagaact ggtggaaaag tttccgcaaa ttgtggtgta
     2581 tggtccacaa gagacacaag ataagggaac aacacaggta gtcaaagatg gcgaaactgc
     2641 cttcgttttg gggcatgaat ttagtgtaat tgccacaccg ggtcacactt taggacatat
     2701 ctgttacttc agtaaacctt atctattttg cggcgacacg ctgttttctg gtgggtgtgg
     2761 taggttgttt gaagggacag catcacaaat gtatcaatca cttaataagt taagtgcgtt
     2821 acctgacgat acattggtat gttgtgctca tgaatatacg ttatcaaata tgaagtttgc
     2881 tttgagtatt cttccgcacg atttgtccat aaatgattat tatcgtaaag ttaaggagtt
     2941 acgggcaaaa aatcaaataa cactacccgt aattctgaaa aatgagcggc aaattaatgt
     3001 ttttttaaga acggaagata ttgatttaat taatgtaatt aatgaagaaa cattattgca
     3061 acaacctgaa gagcgttttg catggttaag gtcaaagaaa gataggttct gataaaactt
     3121 tcttgtcatc ggctccgttc gccgttatga tcggtcgtct tttaagcaac tattgacaca
     3181 cacatgaagg caaaagcgat attactcgcc tctgtcctgc tcgtgggttg ccagagtacc
     3241 ggcaacgttc aacagcacgc acagagcctt tctgcagctg gtcaagggga agcagcaaag
     3301 tttacaagtc aggcacgatg gatggacgat gggacgtcta tcgcgccaga tggtgacttg
     3361 tgggctttca ttggcgacga gctaaagatg ggaattccgg aaaatgaccg gattcgcgaa
     3421 cagaaacaga aatatttacg caataagagc tatctccacg atgtaacttt acgggcagag
     3481 ccgtatatgt actggatagc agggcaagtt aaaaaacgta acatgcctat ggaactggta
     3541 ctactaccca tagtggagag cgcttttgat cctcacgcaa cgtctggcgc caatgccgca
     3601 ggcatctggc agatcattcc gagcacgggg cgcaattatg gtttgaaaca gacccgcaat
     3661 tatgacgcgc gtcgcgatgt tgttgcttca acaactgccg cgctgaatat gatgcagcgt
     3721 ctgaacaaaa tgtttgacgg cgactggctt ctgaccgtag cggcttataa cagcggcgaa
     3781 ggtcgggtca tgaaggcaat taaaacgaac aaagcgcgtg ggaaatccac ggacttctgg
     3841 tcgttaccgt tgccgcagga aacgaagcag tacgtgccta aaatgctggc attgagtgat
     3901 attctcaaaa acagcaagcg ttatggcgta cgtctgccaa cgaccgatga aagccgtgct
     3961 ctggcgcgtg tgcacctgag tagcccggtt gaaatggcga aggttgcaga tatggcgggg
     4021 atttccgtca gcaagctgaa gacattcaac gctggcgtga aaggctccac gctgggcgca
     4081 agtgggccgc agtacgtgat ggtgccaaag aagcatgcag atcaactgcg tgaatctctc
     4141 gcttcaggcg aaattgctgc tgtacagtcg acgctggttg ccgacaatac gccgcttaac
     4201 agccgtgttt acaccgtacg ctctggcgac acgctttcaa gcatcgcttc acgtctcggc
     4261 gtaagcacca aagatttgca gcagtggaac aaactgcgcg gatctaagct gaagccaggc
     4321 caaagtttga cgattggtgc aggcagtagc gcacagcggt tggcaaacaa cagcgatagc
     4381 attacgtatc gtgtgcgcaa aggcgattcg ctttcaagca ttgctaaacg ccacggcgtg
     4441 aacatcaaag atgtgatgcg ctggaacagc gacactgcga atctgcaacc aggcgataag
     4501 ctgacgttgt ttgtgaaaaa caacaacatg ccagattcct gacaaaccag ataataaaaa
     4561 aggcaccgat tcccccggtg ccttttttat ttatgccgct ttatgtgctt ctaccatgat
     4621 gatatcactg gtgaaagagc catcagtctg caaggaaaaa tacgttctca cctctgtcga
     4681 tgcgctctgt tggtaaatgc gaatagcgtc tactaacgct tctggcgtac gcattctcac
     4741 gacccatgaa gaaaattcca gcggtaactt atctgtaatt aaattatcaa ctatcagatt
     4801 ggcttcattg attaacgtca accacgcacc gctggcgtag tttcgtacgt gagaggtatc
     4861 gcgtaatgct tctaccgcct gtaaccagat gtcgcgcact gggtgacccg gagacattac
     4921 atccatcaca atcagcctac cgccaggttt caatatccta ttcacttctc gcagtgctgc
     4981 accaacatca tgccagtgat gggcagaata acggctgata acaatatcaa atgcgttatc
     5041 ggcaaatggc agacttgcgg catatccctg gcgggtggtg atatttttca gttgccgggc
     5101 ttcggcagct tgtgccacga catccagcat gtgggcagat aagtcatacg ccaccaccgc
     5161 gctcacgttt tgcgcagcgc caaacgttgg tctgccctgc ggcagacctg acatacctta
     5221 tttatcaggc ttgccgggac tgaattcaac gagtagcgga ttgtgatcgg aagcgcgcgt
     5281 aaccagtaca gaagcttcac tgacgttcag accacggtag aaaacaaaat cgagcgggcg
     5341 accaaacgcc cgacggcgct gatcatcggt aaaacgcacc tggcgcagcg acatttcccg
     5401 cgcaaagcga tataacgcgt tcatccttct acggctccag gcattgaaat ctcccgccat
     5461 aatgaccggg ccgctgtggt gagctatctg atcaccaata ggaagtaact gcttactata
     5521 gacatccacg cccagactga agttgacggc gtgtatatta accaccatca acaggcgggt
     5581 gtcaggtaat ggatagaccg tcaccagtgc cgacttcgcc agacgcaaaa tgggttctcg
     5641 ttcacgtaac gggcagcaat acactggatg tgccgccgaa agggtcatta cgccagaagg
     5701 atgttgtggc agcacgaaag cgggtacctg atcggcggca agatagttag cggtcgcaaa
     5761 ctgtaccaac tctggcgttg tttgcgcttc ctgcaataac accagatgtg cgtctttgcc
     5821 gtaattcttt aataccgaca accattcagc gcgttgctgt ttgtatatgt tccacaccag
     5881 gatccgaata cgctcttcgg tacttaacgg ttctccgggt ggtaatgcct ggccgatgct
     5941 cgcaaaagac cccggcggta agatcctctc cgcaggttgt ccggcaacat agcgcatggc
     6001 ataggtgttt tttcgcactt ttgactttga aacctctatt acgtcaaccc ttccgcactc
     6061 gggagggttc tactgttata gggattttag ctcactcttt caacgagcaa ttgtatattg
     6121 ttatgtaagc aagtgcttac accggtcaga aatttgctca ttattgagaa cattaaatcg
     6181 gatgacatat cacgcatcct gatggtttaa tgacaaataa attcaaaata tctcatctat
     6241 tttgcaacag gagcaaaaat gaaagccacg tcggaagaac tcgccatttt tgtttcggtc
     6301 gtcgaaagcg gcagctttag ccgggcagcg gaacaattag ggcaagcaaa ctcagcggta
     6361 agccgggcgg tgaaaaagct ggaaatgaaa cttggcgtta gcctgcttaa tcggaccacg
     6421 cggcaactta gcctgacgga agaaggcgag cgttattttc gtcgcgtaca gtcaattttg
     6481 caggagatgg cagcggcaga atcagaaatt atggagacgc gtaatacacc gcgtggactg
     6541 ttacggatcg atgccgcaac tccagtggtg ctgcactttc tgatgccgtt aattaagcct
     6601 ttccgtgaac gctatccgga agtcacttta tcgctagtct cctccgaaac gattattaat
     6661 ttgatcgaaa gaaaagtgga tgtcgcgata cgcgctggta cgttaacgga ctccagctta
     6721 cgtgccaggc cgttatttaa cagttatcga aaaattatcg cctcccccga ttatatttcc
     6781 cgctacggga agccagaaac gatcgacgat ttaaagcaac atgtttgcct gggattcact
     6841 gaacccgctt ccctcaatac ctggccgata gcctgtagtg atggacaatt acatgaggtg
     6901 aagtacggtt tgtcatccaa tagtggggaa acactgaaac agctttgcct gagtggcaac
     6961 gggattgcgt gtttgtccga ctacatgatc gacaaagaaa tcgctcgcgg agaattggtg
     7021 gagttaatgg cagataaagt gttgccagtg gaaatgccat tcagtgccgt ctattacagc
     7081 gaccgtgcgg taagtacgcg catccgggct tttatcgatt tccttagcga gcatgtaaaa
     7141 acagctcccg gaggagctgt cagagaggct taatcccatt caggagccag accttccggg
     7201 ctaaccaggc ggtcgttgca atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc
     7261 tgtaaatttt gtgccttaag attactttcc aggtttttac gtttagtaga agaaggaatt
     7321 actgagtaac cttcccccat agcccacgcc agaatcactt gtgccggagt cgcattgtgt
     7381 ttagctgcaa tacgagcaat aacctcatct ttcagtgcct taccataagc cagcgtcata
     7441 taggaagtga tatgaatacc gtgctgttta gcccaggcaa ccactttacg gttttgcaga
     7501 taaggagaga gttcaatctg gttggtagcg atgttttcag cgccaacagc agcaatcgct
     7561 ttttccatca acgggatcgt gaagttggaa ataccgatct cacgtgtcag accttgtttt
     7621 ttggcctcca gcagcgcctg cataaactct tcaacagaga cttcatcgtt tggtgacggc
     7681 cagtggatta gcgtcagatc aacataatcg gtacgcaatt tttgcaggct ctcttttaga
     7741 ctcgggatca atttgtcttt gctgagattt tcaatccaga ttttagtggt gatgtagagt
     7801 tcatgacgtg gcacgccact ttctgcaatc gcctgaccta ctgcggcttc gttatcatag
     7861 atttgtgcag tatcaattgc gcgataacca agttcaagcg ccgttttcac agatgaaata
     7921 acaacgtcgt ctttgagacg gaaagtacct aaaccaaatg cagggatagc cataatatgc
     7981 ctcttttaat gctattttcg ttaactgagg tgattatggg cttcagactg ttgcggaaaa
     8041 agagcataaa atgcagagga tttttgcgat tctggcaata atagatatac agacgaaaaa
     8101 aaacctcgag cgttaactca aggcttctta ataagtggcg gaacggacgg gactcgaacc
     8161 cgcgaccccc tgcgtgacag gcaggtattc taaccgactg aactaccgct ccaccgaatt
     8221 cttttacaac caccggtttt atgaccggct tactgcttaa tttgatgcct ggcagtttat
     8281 gaatcacttc gtgattcacc cttcgggccg ttgcttcgca acgttcaaaa gctttcgctt
     8341 ttgtcctact ctcttatcga gtagtgaact gcctcaaagg tcagtgccat tcttc
//
LOCUS       DAZPDU010000178         8358 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZPDU010000178 DAZPDU010000000
VERSION     DAZPDU010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8358
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.180"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..189
                     /locus_tag="ACHURS_003697"
     CDS             <1..189
                     /locus_tag="ACHURS_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239327.1"
                     /translation="DMLNPTKDTNWNSTCIYKSRHKMLPINLTQETLFSSKSHGKYAL
                     FPIFTASWRATRIKNIGI"
     gene            412..909
                     /gene="ubiC"
                     /locus_tag="ACHURS_003698"
     CDS             412..909
                     /gene="ubiC"
                     /locus_tag="ACHURS_003698"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFR1239328.1"
                     /translation="MSHPALTQLRALRYFKEIPALDSQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            922..1794
                     /gene="ubiA"
                     /locus_tag="ACHURS_003699"
     CDS             922..1794
                     /gene="ubiA"
                     /locus_tag="ACHURS_003699"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFR1239329.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(1949..4372)
                     /gene="plsB"
                     /locus_tag="ACHURS_003700"
     CDS             complement(1949..4372)
                     /gene="plsB"
                     /locus_tag="ACHURS_003700"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFR1239330.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRPLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4543..4911
                     /gene="dgkA"
                     /locus_tag="ACHURS_003701"
     CDS             4543..4911
                     /gene="dgkA"
                     /locus_tag="ACHURS_003701"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFR1239331.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5021..5629
                     /gene="lexA"
                     /locus_tag="ACHURS_003702"
     CDS             5021..5629
                     /gene="lexA"
                     /locus_tag="ACHURS_003702"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFR1239332.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5702..7027
                     /gene="dinF"
                     /locus_tag="ACHURS_003703"
     CDS             5702..7027
                     /gene="dinF"
                     /locus_tag="ACHURS_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFR1239333.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMSKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7143..7352
                     /locus_tag="ACHURS_003704"
     CDS             7143..7352
                     /locus_tag="ACHURS_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFR1239334.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7394..7909)
                     /gene="zur"
                     /locus_tag="ACHURS_003705"
     CDS             complement(7394..7909)
                     /gene="zur"
                     /locus_tag="ACHURS_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFR1239335.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            8227..>8358
                     /gene="yjbL"
                     /locus_tag="ACHURS_003706"
     CDS             8227..>8358
                     /gene="yjbL"
                     /locus_tag="ACHURS_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjbL"
                     /protein_id="HFR1239336.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFDNEDAIKDKLDNRGSIKGKLNNI
                     "
BASE COUNT         1934 a         2014 c         2263 g         2147 t
ORIGIN      
        1 gatatgttga atcctacaaa agataccaat tggaattcca cgtgtatcta taaatccaga
       61 cataagatgt tgcctattaa tcttactcag gaaacacttt ttagctccaa atctcatggt
      121 aaatatgcgc ttttcccaat ttttactgcg agttggcgag ctactcgtat aaagaatata
      181 ggtatttaag taaaggaaaa catcaccgtt cctggcatca tgggcggtga tgcccctacg
      241 gttgccctcg ccagcacggg catcggcaaa gcgtaaggtt caacagcgtt ttaccacttc
      301 atgcgattgt tgcgtttttg ttgcgcatta gatcacttaa tttgctttac atctcccgta
      361 aacacttttc tgcgatacaa tgcctttacg ttatgtaacg gagagttcgg catgtcacac
      421 cccgcgttaa cgcaactgcg tgcgctgcgc tattttaaag agatccctgc cctggattcg
      481 caactgctcg actggctgtt gctggaagat tccatgacaa aacgttttga acagcaggga
      541 aaaacggtga gcgtgacgat gatccgcgaa gggtttgtcg agcagaatga aatccccgaa
      601 gaactgccgc tgctgccgaa agagtctcgt tactggttac gtgaaatttt gttatgtgcc
      661 gatggtgaac cgtggcttgc cggtcgtacc gtcgttcctg tgtcaacgtt aagcgggccg
      721 gagctggcgt tacaaaaatt gggtaaaacg ccgctaggac gctatctgtt cacatcatcg
      781 acattaaccc gggactttat tgagataggc cgtgatgccg gactgtgggg gcgacgttcc
      841 cgcctgcgat taagcggtaa accgctgttg ctaacagaac tgtttttacc ggcgtcaccg
      901 ttgtactaag aggaaaaaaa tatggagtgg agtctgacgc agaataagct gctggcgttt
      961 catcgcttaa tgcgtacgga taagccaatt ggcgcgttac tgctgctctg gccaacatta
     1021 tgggcgttgt gggtggcgac accgggcgtt ccccagctct ggatcctggc ggtgtttgtc
     1081 gcgggtgtct ggctgatgcg cgctgccgga tgtgtggtga atgattatgc tgaccgcaag
     1141 tttgatggtc atgttaagcg cacggcgaac cgaccgcttc ccagcggcgc ggtaacggag
     1201 aaagaggcgc gcgcgctgtt tgtagtgctg gtattgattt catttttact ggtgctgacg
     1261 ctgaatacga tgaccattct gttgtcgatt gccgctctgg cgttggcgtg ggtgtacccg
     1321 tttatgaagc ggtataccca tctcccgcaa gtggtgctgg gcgcggcgtt tggctggtcg
     1381 attccaatgg cttttgccgc tgtgagtgag tcggtgccat tgagttgctg gttaatgttc
     1441 ctcgccaata ttctctgggc ggtggcttac gacacgcagt atgcaatggt tgaccgtgac
     1501 gatgatgtga agattggtat taaatccacc gcaatcctgt tcggccaata cgataaattg
     1561 attattggta ttttgcagat tggcgtactg gcactgatgg cgatcatcgg tgagttaaat
     1621 ggcttaggct ggggatatta ctggtcaatt ctggtggctg gcgcgctgtt tgtttatcaa
     1681 caaaaactga ttgccaaccg cgagcgtgaa gcctgcttta aagcatttat gaataataac
     1741 tatgttggtc tggtactgtt tttaggactg gcaatgagtt actggcattt ctgatgatgt
     1801 aaaaaagccg gatgatcatc cggctttctt ctgggttgcc tgatgcgctg cgcttctcag
     1861 gcctacacaa cacatcgcaa tttattgaat ttgcagatta tggaaggccg gataaggcgt
     1921 tttcgccgca tccggcaatt ctctctgatt acccttcgcc ctgcgtcgca ctctcaatcg
     1981 tcaaacgcac gtctgatgta atcaactccg ccagcaactg ataaaccttc atcgtttctg
     2041 ccggttcggc gtcgccgcta tcgctgatat acccttcatc acgcagcgtc agcaccagag
     2101 aactgaacac cgccttgtcg aagaactccg gcgcgttgat gccgtgcagc acggagagac
     2161 gttgcgcaac ggtgcggctc tctttctcca gcgtaccgcg gttgatcgac gggttggcac
     2221 tcaacaacca gaaggtgatg gcataacgtt gcagcgtttc gcgcgcgcct gcggccagca
     2281 gctgtagggg gcgagaatgc gccgggttga tatgcaactc atcatcttgc agggtaatca
     2341 gcccctgacg ttgcatctca tttgccagcg catcaataac gtccggcaac tcgtcgcgat
     2401 cccagcgcag gaacagctcc gctttcagca ttgggtaaag cacattgacg tgctccatca
     2461 atacgtcgcg ggagatgtgg cgatgctggg tgacaattgc cgccatcagc gaaggcagca
     2521 ccaacatatg cgcaatgttg ttgcgatagt aggtcatcag caccgcttgc tcgcgcggca
     2581 gaatgatgat gtcgccgata gtgtctttct cgacttcaaa cttgttcatt tgcagcgcgt
     2641 gatcgataag ctcgctggcg ctggctgaag gaacggtaga gtccgtggag taaggtacgt
     2701 tgcgcatcag atccaggtag cagttgagtt gctcggttaa ctgctcgcgg gtgagtgagc
     2761 gctgacgtga tgccagaagc gcagtacaac acaggttcat ggcgtttgcc gcgcctgcgt
     2821 tgttaatgcg taccatcaga tcggcagcaa tattattgac cgtcggcgtt aaccaggccg
     2881 gacgcaccgc ttcgatggga tcgatagatt cacgccagtc tggtacgtgc tggttaaggt
     2941 aggtcatcaa cggcattggt tcaccgaagt tgacgtaacc ctgaccgaga ttacgcagct
     3001 tgcttaaacc gcgcagcatc tgcggcaggc tctctttctc tttcgtcgcg ccgcgcagtt
     3061 ctttggcgta agtacccact tccatgacgt gctcataacc gatatagatc ggaatcagcg
     3121 taatcggacg cgtgccgcca cgcagcatcg cctgaatggt catcgacagc gtaccggttt
     3181 tcggatccag caaacgcccc gtgcgggaac gaccgccttc cacgaagtac tcgacggaat
     3241 aaccacggct gaacagttcg ccgagatact cacggaaaac ggtggaataa agtttattgc
     3301 ctttaaaagt acggcgaata aagaacgccc ccagacggcg gaaaatcggc ccggccggcc
     3361 agaagttcag gttgatcccg gcggcgatat gcggcggcac cagcccctgg tggtacagca
     3421 cgtaagaaag tagcaggtag tccatgtgac tgcggtggca aggcacatac accagctcat
     3481 gaccgtcgtg ggccagctgg cgaacgcgct cggcgttatg gacgttgatg ccctgataaa
     3541 gtcggttcca ggtgaagccc agaatgcggt cagtcaggcg gatcatctcg taagagaaat
     3601 tcgccgcaat ctcttccatc agcgcaatcg cgttctgctg cgctttttca tgggagattt
     3661 ttttgctgcg cgcttcatct tctaccgctt tggcaatggc gcgggaggcg agcagcttat
     3721 taaacagatc ctgacgagca ggcagacgtg ggcctacggc agccagacgt tgacgggcaa
     3781 agtgcatacg cgccacgcgc gccagtttct gtgcgatagt tttatccgtg ccgtgttcat
     3841 ccgccatacg gcgcagcgaa actgacggcg agaaacgcac aaaactgtcg cgaccgagcc
     3901 acagtacggc gaaaaatttc tgtacgccgt taagcatacg cagcggcggg tttacttcgc
     3961 ctttttcacg ccccggcgcg cgaccaaaca tcaccgatac tggcaccatc tgcacatcca
     4021 gatttgggtt gctacggtgc aaatcgagat agtcgtggaa cagcttaata gactcttctt
     4081 tcggcgtgta ataggtgaat acacgcggcc cgccgtgaat gaacacatag cgcggcagta
     4141 gcgtgccgtc gatttctagc ggctctaacg ggtcaggcaa gtcatgagcc agacactggg
     4201 cgcgtaacgt cagcaaatct gctttcgagt tgtacggtaa aacgtacata attggacgag
     4261 aggtatccag ccccagttcc ggggcaggat ctgccggaat agacttgctt tttaccagga
     4321 tgcttaatgg taaattcagt aatttgtagt aaattcgtgg ccagccggac ataaacgatg
     4381 taaagcctct ggttaataat gcaaatgcgc ggcaaggata gcagaaagtc atgggaaatt
     4441 ctgtggtatc cgctcatgtt tcgcgcggcg ttacgcaaac ccgaatcatc ggatttaacg
     4501 gtacactgat attgacgctc ataatgtaaa aaggttcttt caatggccaa taataccact
     4561 ggattcaccc gaattatcaa agctgctggc tattcctgga aaggtttacg cgctgcatgg
     4621 atcaacgaag cggcattccg tcaggaaggc gtagcggtat tgttggcggt ggtcatcgcc
     4681 tgctggctgg atgtggacgc aattacccgc gtgctgctta tcagctccgt gatgctggtg
     4741 atgattgtgg aaattctcaa tagcgccatc gaagcagtgg ttgaccgaat tggctctgaa
     4801 taccatgagc tttccggacg cgctaaagat atgggatccg ccgcggtgct gattgcgatt
     4861 atcgtcgccg tgataacctg gtgcatcctg ttatggtcgc attttggata acccttccag
     4921 atttcgataa atctctggtt tattgtgcag tttatggttc caaaatcgcc ttttgctgta
     4981 tatactcaca gcataactgt atatacaccc agggggcgga atgaaagcgt taacggccag
     5041 gcaacaagag gtgtttgatc tcatccgtga tcacatcagc cagacaggta tgccgccgac
     5101 gcgtgcggaa atcgcgcagc gtttggggtt ccgttcccca aacgcggctg aagaacatct
     5161 gaaggcgctg gcacgcaaag gcgttattga aattgtttcc ggcgcatcac gcgggattcg
     5221 tctgttgcag gaagaggaag aagggttgcc gctggtaggt cgtgtggctg ccggtgaacc
     5281 gcttctggcg cagcagcata ttgaaggtca ttatcaggtc gatccttcct tgttcaagcc
     5341 gaatgctgat ttcctgctgc gcgtcagcgg gatgtcgatg aaagatatcg gcattatgga
     5401 tggtgacttg ctggcagtgc ataaaactca ggatgtacgt aacggtcagg tcgttgtcgc
     5461 acgtattgat gacgaagtta ccgttaagcg cctgaaaaaa cagggtaata aagtcgaact
     5521 gttgccagaa aatagcgagt ttaaaccaat tgtcgttgac cttcgtcagc agagcttcac
     5581 cattgaaggg ctggcggttg gggttattcg caacggcgac tggctgtaac atatctctga
     5641 gaccgcgatg ccgccaggcg tcgcggtttg tttttcatct ctcttcatca ggcttgtctg
     5701 catggcattc ctcacttcat ctgataaagc actctggcat ctcgccttac ccatgatttt
     5761 ctccaatatc accgttccgt tgctgggact ggtcgatacg gcggtaattg gtcatcttga
     5821 tagcccggtt tatttgggcg gcgtggcggt tggtgcaacg gcgaccagct ttctctttat
     5881 gctgttgctg tttttacgca tgagcaccac cgggctgact gcgcaggctt atggtgccaa
     5941 aaatcctcag gcattagccc gtgcgctggt gcaaccgttg ctgttggcgt tgggggctgg
     6001 ggcgttaatt gcgctgctgc gtacaccgat tatcgatctg gctctgcata ttgttggcgg
     6061 tagtgaagca gtcctggaac aggcgcggcg ctttcttgaa atccgctggt taagcgcacc
     6121 ggcgtcgctg gcgaatctgg tattactcgg ttggttactc ggcgtgcaat atgcccgtgc
     6181 gccagtaatt ttgttagtgg tcggcaatat cctcaacatt gtgctggatg tctggctagt
     6241 gatggggctg catatgaacg tgcagggcgc ggctctggcg acggttattg cggaatatgc
     6301 aacattgctg attggtctgc taatggtgcg taaaatcctc aaactacgcg gaatttccgg
     6361 cgaaatgtcg aaaactgcct ggcgaggaaa cttccgtcgc ttgctggcgc ttaaccgcga
     6421 tatcatgctg cgttcgctgt tgttgcaact ctgtttcggc gcgatcaccg tacttggcgc
     6481 gcgactgggg agtgacatta tcgctgtaaa cgcggttctg atgacgctac tcacctttac
     6541 cgcctatgcg ctggatggtt ttgcctacgc ggttgaagca cattccggtc aggcatacgg
     6601 tgcgcgcgac ggtagccagt tgctggatgt ctggcgggca gcgtgccgcc agtcggggat
     6661 cgtagcgtta ctattttcgg tggtttattt gctggcaggg gaacacatca ttgcgttgct
     6721 gacgtcgtta acccagattc agcagctggc tgaccgctat cttatctggc aggtgatttt
     6781 gccgttggtt ggcgtctggt gttatctgct ggacggcatg tttataggcg caacgcgtgc
     6841 cgccgaaatg cgtaacagta tggcggtggc cgccgcaggt tttgcgctga cgctccttac
     6901 gctgccgtgg ctgggtaatc atggtttgtg gctggcatta accgtctttc tggcgttgcg
     6961 cgggctttct ctggcggcta tctggcggcg tcactggcgt aacggtacct ggtttgccgc
     7021 aacgtgacgg ttaaaaattc tgaataaatt atcctaagcc aaattgctga ctacacttaa
     7081 tctcacgttc agaagaaaag tgaacgtact ctcattcaca acctaacgat gaggtcttga
     7141 ttatgaataa agatgaagcc ggcggtaact ggaaacagtt taaaggtaaa gtgaaagagc
     7201 aatggggcaa actgaccgat gatgatatga cgatcattga aggtaaacgt gatcaactgg
     7261 tcggtaaaat ccaggaacgt tatggttatc agaaagatca ggcagaaaaa gaggtcgtgg
     7321 attgggaaac ccgcaatgaa tatcgctggt aattaatccc tcctgcccga cgtgtacaag
     7381 gatgtacgcc ctcttaacgc ggtttctttt tcacctgcac agagtgatca tgctggcact
     7441 gttcaggatg acgacacgct tccacttcca cgcatgccgc acagagccca tgtgcttcaa
     7501 tcacattatg ccgcagggca aaccccattt ttgccgccag cgtatgcata atgtcttcca
     7561 cgccttctgc acactcttct ttcaccgcgc cgcagcgatc gcatataaac atggctgacg
     7621 tatgcgtggg ctggtcgaac agatgacaga gcacataact gttggtggat tccaccttat
     7681 gcacaaaacc ttgctcaagc agaaaatcca gcgcgcgata aaccgttggc ggcttggctt
     7741 gcggttcagc ttcgcgcagt aaatcaagca gatcataagc gctgatagcg ccatcctgca
     7801 gactcatcag gcgcaacact tccaggcgct gtggggtcag gcgcacatta cgctgcgcgc
     7861 agattttttc agcctgcgct aataactcct gcgttgtggt cttttccatc gggcacctca
     7921 aagtgggggt taagaatgtc cttactttac catgttccag gaaaaacacc gagaaccctt
     7981 attgttgccg taatgttgat tttctgtttt gtaggtaagg tgttatgttg ccttgtcgta
     8041 ccattatcaa cacgataata attaataatt catttttaaa taaattgctt caagttaact
     8101 cgctatttat ttaactgggt ttaaacgcat acctgtagtt cagaaattat gagcgatttt
     8161 tgatagtgac cagatggcaa aagaattata aggaggacac ggtacgacaa aaaaggtaga
     8221 tgaatcatgt tgaagataat acccggtgca actggatact ttaataaaac actcaattca
     8281 aatcaattcg ataatgaaga tgcaatcaaa gataaattag ataatagagg ttcaatcaaa
     8341 ggtaaattaa ataatata
//
LOCUS       DAZPDU010000179         8326 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZPDU010000179 DAZPDU010000000
VERSION     DAZPDU010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8326
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.181"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..135)
                     /locus_tag="ACHURS_003707"
     CDS             complement(<1..135)
                     /locus_tag="ACHURS_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbI"
                     /protein_id="HFR1239337.1"
                     /translation="MTTTQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     P"
     gene            complement(132..2762)
                     /gene="traC"
                     /locus_tag="ACHURS_003708"
     CDS             complement(132..2762)
                     /gene="traC"
                     /locus_tag="ACHURS_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HFR1239338.1"
                     /translation="MSNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRL
                     LPYRDYNQESGLFMNDTTMGFMLEAIPINGANKSIVEALDHMLRTKLPRGIPLCIHLM
                     SSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAATQFPLPEGMNLPLTLRHYR
                     VFISYCSPSKKKSRADILEMENLVKIIRASFHGAKITTQTVDAQAFIEIVGEMINHNP
                     DSLYPKRRQLDPYSDLNYQCVEDSFDLNVRADYLTLGLRENGRNSTARILNFHLARNP
                     EIAFLWNMADNYSNLLNPEMSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTS
                     YAKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSF
                     RKNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADN
                     PLTPAGLLAPTYRNQLAFIDIFFKGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSG
                     GFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPN
                     GNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAGSPTIRSRLDEMI
                     VLLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENR
                     MYRTPRTLKKLNVIDEGWRLLDFKNRKVGEFIQKGYRTCRRHTGAYITITQNIVDFDS
                     DKASSAARAAWGNSSYKIILKQSAKEFAKYNQLFPDQFQPLQRDMIGKFGAAKDQWFS
                     SFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRREGLSIHEAVWQLAWKKSG
                     PEMASLEAWLEEHEKYRSVA"
     gene            complement(2888..3235)
                     /locus_tag="ACHURS_003709"
     CDS             complement(2888..3235)
                     /locus_tag="ACHURS_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239339.1"
                     /translation="MKSIYLKSVLAFIFVGVMAMIVCIPFYIVYLAQQPATPEQLTEI
                     LQETPCAAEAFQETLNYQSEPLTLGKANKIASECRKRNEMAEVKRVRENERNKIREKQ
                     IQALNDAHSVKER"
     gene            complement(3263..3481)
                     /locus_tag="ACHURS_003710"
     CDS             complement(3263..3481)
                     /locus_tag="ACHURS_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239340.1"
                     /translation="MHTFIFLVLIKNINGGGSWNITPMPNTDVCVQVLRTIEAHGGRG
                     FGNTGECIEVKTNEPLNSVMETLNGGGQ"
     gene            complement(3580..4056)
                     /locus_tag="ACHURS_003711"
     CDS             complement(3580..4056)
                     /locus_tag="ACHURS_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239341.1"
                     /translation="MHKSENEKIDFILDEHERISRKQGSLCMALGKGFLVIAILCGVA
                     TFCVSGLYLKSLCLSVACCCGVFSRIFNYYSVPEESRMVLSRQELLRLMRLTEDVPDV
                     RQELLNRLLSGKKLTGRDEKDIRRLWQEKVDAMQESATRQREQDTIRKFAEESKVC"
     gene            complement(4049..4270)
                     /gene="traR"
                     /locus_tag="ACHURS_003712"
     CDS             complement(4049..4270)
                     /gene="traR"
                     /locus_tag="ACHURS_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HFR1239342.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQEINVHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            complement(4405..4920)
                     /gene="traV"
                     /locus_tag="ACHURS_003713"
     CDS             complement(4405..4920)
                     /gene="traV"
                     /locus_tag="ACHURS_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HFR1239343.1"
                     /translation="MKQISLFIPLLGTLLLSGCAGTNTEFECNATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLPRLAEGNFRTMPVQTVTATTPSGSRPAVTAHPEQKLLAPR
                     PLFTAAREVKTVVPVSSVTPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            complement(4917..5237)
                     /locus_tag="ACHURS_003714"
     CDS             complement(4917..5237)
                     /locus_tag="ACHURS_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HFR1239344.1"
                     /translation="MNMRNINVITARVVPDKNVSDDFMHAVLSNCTTKIVLPAPKVFS
                     SESLPHKFNMAAVGVMKKGMSMSLFSSPGNTVVSGHPCAGKTNLTAELISAVVGSNKK
                     GEGQ"
     gene            complement(5224..5814)
                     /gene="traP"
                     /locus_tag="ACHURS_003715"
     CDS             complement(5224..5814)
                     /gene="traP"
                     /locus_tag="ACHURS_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus-stabilizing protein
                     TraP"
                     /protein_id="HFR1239345.1"
                     /translation="MANNMSSRQACHAARYVVVRVLRGLFWCLKYTVILPLATMALMA
                     LFVLWKDNTTPGKLLVKEINFVRQTAPAGQFPVSECWFSSSDSSGRSEIQDICHYRAA
                     DSADYVRETDRSLMQLVTALWATLALMYVSLAAITGKYPVLPGKMKCIRVVTADEYLK
                     DVYTEDASLPGKIRKCHVYFPDDRTNRSNGDKNEHA"
     gene            complement(5804..7231)
                     /gene="traB"
                     /locus_tag="ACHURS_003716"
     CDS             complement(5804..7231)
                     /gene="traB"
                     /locus_tag="ACHURS_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer pilus assembly protein
                     TraB"
                     /protein_id="HFR1239346.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGAASAIGGVLYLSDVDMSGNGEA
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQMPASPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVTYQSVPVPNRIQRKVFTRNEGKQGPSLPYIPSGS
                     FAKAMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVDG
                     IGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGASKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALERTQSRAEEDNPESPVPVPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGSQGGGSDGK"
     gene            complement(7231..7959)
                     /gene="traK"
                     /locus_tag="ACHURS_003717"
     CDS             complement(7231..7959)
                     /gene="traK"
                     /locus_tag="ACHURS_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HFR1239347.1"
                     /translation="MRKNNTAIIFGGLFFSCSVMAANGTLAPTVVPMVNGGQASIAIS
                     NTSPNLFTVPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLS
                     IQAVPREGAGRTIQLVSDLRGTGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQV
                     PVTKETLQAPAGLSSVADAVWTGNHLKMVRFAVENKTLSALNIRESDFWQPGTRAVMF
                     SQPASQLLAGARMDVYVIRDGEGN"
     gene            complement(7946..>8326)
                     /gene="traE"
                     /locus_tag="ACHURS_003718"
     CDS             complement(7946..>8326)
                     /gene="traE"
                     /locus_tag="ACHURS_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFR1239348.1"
                     /translation="NSADASYLQQMALSFIALRLNVSPEIVDASHQALLQYIRPGAQN
                     QMKVILAEEAKLIKKDNVNSAFFQTSVRVWPQYGRVEIRGIRKTWIGNSEPFTDIKHY
                     ILILKRENGVTWLDNFGETDDEKK"
BASE COUNT         1918 a         2311 c         1992 g         2105 t
ORIGIN      
        1 cggtgcgttc agtctgacaa tgccgtaact gacggctgcg ttcagtaaca ccattgccag
       61 gcatcccggc accgtccacc accagtggct gcgtcggggg gctgtaacat cagtcgtttt
      121 ttgcgttgtg gtcatgcaac actcctgtat ttctcatgtt cttccagcca ggcttccagc
      181 gaagccatct ccggccctga cttcttccac gccagctgcc acaccgcctc atgaatactc
      241 aggccctccc tgcgtttctg ctgcacaaac tcaaaatccg ggccgtcaga gctgtacatg
      301 gcgcggctta acgggtccac aaacagacgg tgccaggagg aatggttttc cacctgcagc
      361 aggaaggaac tgaaccactg gtctttggcc gcaccaaact tgccaatcat gtcgcgctgc
      421 agtggctgga actgatccgg gaataactgg ttatatttcg cgaactcctt cgcactctgt
      481 ttgaggataa ttttgtagga ggagttaccc catgccgcac gggccgcact ggaggccttg
      541 tcagagtcaa agtcgacgat gttctgtgtg atggtgatat aggcaccggt atggcggcga
      601 caggtgcggt aacctttttg aataaactca ccgactttac gatttttgaa gtccagcaga
      661 cgccagcctt catcaatgac gttcagtttc ttgagagtac gcggcgtgcg gtacatcctg
      721 ttctcgatgt agataatcag ggagaacatc accgcaacca gcagtgacgg acggtcttcc
      781 agtccgccca gctccagcac caccattttc gcatcatccc gcagggacgg ctcatcggag
      841 ttaaaatact ggccgtaagt gccgttggca gtgtactggt caagcagcac aatcatttcg
      901 tccagacggc tgcggatggt cggtgacccg gcatactgct cgctgtcact ggcgtttttc
      961 aggaaatcca ccacgtcatc aatacgtgcc ctgttctctt tggccagcca ggaggccctg
     1021 accgcctgca gcagcagacc ttcatgcact tcatccaggt taccgttggg gctggccatc
     1081 actgacaact ggtcacggac acgctccgct gactggtcaa tatcggtgat gttcgcaaac
     1141 gggttaaaac gcagggtttc accgtccaga tatacgcccc ccatgttctc acacagggac
     1201 ttgtagccat cccccatgtc gaacaccacg gcaaagcccc cggagtccag cacgctgcgg
     1261 ataagtggct gtatcagtcc ggttttaccg gccccggagg tgccacagac cgccatgttg
     1321 tagttggtgt tattcatccc cttaaagaag atatcgataa acgccagctg gttacggtag
     1381 gtgggcgcca gcagacctgc cggtgtcagg gggttatccg ccactaacgg catcaggttg
     1441 gccacattaa agctctctgc gcgctgaacc acaccggcct ctttcagctg tttaaacagc
     1501 cctttcccgg ccataaaggg caggcaggtc aggaaattgc gcatgtggtt aaagcgcggt
     1561 gaaatcagct caaaaccgtt tttacggaag ctgttcagga tgtcctgctc cacttccagt
     1621 gccgtttcat tattgtcctt gcagaaggct gtgatgttga ggaaatagga cacgacagaa
     1681 gactggccgg agcccagccg ctggcgcagt tccccccact ccttcgcctc tttctccacg
     1741 gacggaaacc atttggcata ggaggtcttc gacttcttct ccaggtccat gtacttcagg
     1801 ttggcttcgc tgtgggtttt cacctggtct tccaccacca gagtcagcgt caggatgaac
     1861 gggcaggaaa tggacatttc cgggttcagc agattgctgt agttgtcggc catgttccac
     1921 aggaaggcga tttccgggtt acgggccaga tggaaattca ggatgcgggc cgtgctgttc
     1981 ctgccgttct cacgcaggcc cagcgtcagg taatcagccc ggacattcag gtcaaaactg
     2041 tcctccacac actgataatt cagatcagaa tacgggtcca gctgacgtct tttcgggtac
     2101 agagagtccg gattatggtt aatcatctcc ccgacaatct cgatgaaagc ctgtgcatcc
     2161 actgtctgtg tggtgatttt tgcgccgtga aaagaggcac ggatgatttt caccagattt
     2221 tccatttcca gaatgtcagc ccggcttttt ttcttcgagg gagaacagta cgagataaac
     2281 acccggtaat ggcgcagggt caggggcaga ttcatcccct ccggcagcgg gaactgtgtc
     2341 gctgccgctt tcatataata ggcacgggta atggcgttaa accgttcagc ctgttcacct
     2401 gaccaggaga actcacgcag cccgtattca atcctgtcac caaccagctg actggacatc
     2461 agatggatac acaacggaat accgcgcggc agtttggtgc gcagcatgtg atcgagtgcc
     2521 tccacaatgg acttattcgc cccattgatg ggaatggctt ccagcataaa gcccatcgtg
     2581 gtgtcattca tgaacagacc ggattcctgg ttgtaatcac ggtacggcag cagacggctg
     2641 aactgcggga agctcatttc gcccagaact tcattggcct ttgcggattc gtcaggcagt
     2701 ttcagtgctg tgacgaggga gttaaccgcc tgagtgacgg cctcaagtgg gttattactc
     2761 atatgagtat tccttagtgc gtttaaaaaa ggaaaatatc agtctgtggc cgtggatata
     2821 tcacgcagtg ccattcctga aaccacagca caaataagag atggaggctt ttaacaggaa
     2881 accaggatta acgttccttt actgaatgag catcatttaa tgcctgaatt tgtttttcgc
     2941 gaatcttatt gcgctcattt tctcttaccc gtttcacctc tgccatttcg tttctttttc
     3001 tgcattctga tgcgatttta tttgccttac caagtgttaa aggttcagac tgataattaa
     3061 gtgtctcctg aaatgcttct gctgcacagg gagtttcctg taaaatctct gtcagttgtt
     3121 ctggtgttgc aggttgttgc gcaaggtaaa cgatgtaaaa aggaatacat acaatcatag
     3181 ccataacgcc aacaaatata aaagccagaa cagattttaa atatatcgac ttcatttcat
     3241 tttccttcga agcagtaagc actcattgcc caccaccatt tagtgtttcc atcacgctgt
     3301 tcaaaggttc atttgtttta acttcaatac actcccctgt atttccaaag cccctgccgc
     3361 catgtgcttc gattgtccga agaacctgca cgcatacgtc cgtatttggc atgggtgtaa
     3421 tattccagct tccgccaccg ttaatatttt ttatcagcac aagaaaaata aaggtatgca
     3481 ttctttatta tctccgcttg tatattaatc actgaatatc ccttcagtta acttatgagg
     3541 tcataaaaca ctgcagattt tcattcagat ttactctctt cagcaaactt tactctcttc
     3601 agcaaacttc cggatggtat cctgttcacg ctgacgggtg gcagactcct gcattgcatc
     3661 cactttctcc tgccagagac gccggatatc cttttcatcc cgaccagtca gttttttacc
     3721 ggacaacaaa cggttaagca gttcctgtct cacatctggt acgtcttcag tcagacgcat
     3781 aagccggagt aattcctgac gactgagtac catgcgtgat tcctccggta cgctgtaata
     3841 attaaaaata cgactgaata ccccacaaca gcaggcaacg gacaggcaca gggatttcag
     3901 atacaggcca gaaacacaga atgtggcaac accacaaaga atagctataa ctaaaaatcc
     3961 cttacccagt gccatacaca aagaaccctg ttttctggat atccgctcat gctcatccag
     4021 aataaaatct attttttcat tttctgattt atgcatagtg tttcctctgc ctttcctgat
     4081 acgcctgaca ttcaacgcac aacgtcacac cgggaaatat tttccgccgg gcttccggaa
     4141 taggatttcc gcatgcttca cagagataaa caggaatacc atgaacattt atttcctggc
     4201 gtatccggtt tattcctgtc atggtcagtt gttctgtcac tgaatatgct tcatcggctt
     4261 catcactcac ggaatattcc tcccgacatg ttctgacacg ctaccgccca tcccttttca
     4321 cgcacagggc actcgtttta caggcggatt ttgaatacag aaagcccgcc ggcattgccg
     4381 gctgatatac aacttcaggg ccagttaatt aatacgtggt tttccccacg cagacggttt
     4441 aataacaaaa aacacgctgg atggctgatg ataaacatcc tggttatcaa tataaggcgc
     4501 tatccataat gctgccgtct gctcacccgt tcttagcgga cgaggaggtg ttaccggcgt
     4561 aactgaactg accggaacaa ccgttttcac ttcccgggca gcggtaaaca gcgggcgtgg
     4621 tgccagcagt ttctgctccg gatgtgctgt caccgcaggt ctgctgccgg acggagtggt
     4681 cgcggtgacg gtctgcaccg gcattgtccg gaagttccct tcagccaggc gcggcagtga
     4741 tgccgcaacc ggctttgctt ctgaggactg ctccagtttt ttggccttct cattggcctg
     4801 ctccatcgtc atacaggtat cggatgtggt ggcgttacac tcaaattccg tatttgttcc
     4861 tgcgcagccg gaaagtaata aggttcccag cagaggaata aataaagaaa tctgtttcat
     4921 tgcccttctc cttttttatt actccccaca acagcagaaa ttaactctgc tgtcagattt
     4981 gttttccctg cacagggatg tccggagacc actgtatttc ccggagaaga aaataagctc
     5041 atcgacattc ccttcttcat cacaccgaca gcagccatat taaatttgtg cggcaatgat
     5101 tcagaactga acaccttcgg agcaggaaga actatttttg tggtgcagtt actgagaaca
     5161 gcatgcataa aatcatctga tacgttttta tccggcacaa cgcgggctgt aataacatta
     5221 atattacgca tgttcatttt tatctccgtt gcttctgttt gttcggtcat cagggaaata
     5281 aacatggcat ttcctgattt ttcccggcag ggaagcgtct tccgtataaa cgtccttcag
     5341 atattcatct gcggttacca cccggataca tttcattttt ccgggcagca ccggatattt
     5401 cccggtgatc gctgccagcg agacatacat cagtgccagt gttgcccata atgcggtgac
     5461 cagttgcatc agtgaccggt cagtctccct gacataatct gcagaatcag ccgcgcggta
     5521 atggcagata tcctgtattt ctgatctgcc tgaagaatca gaggaagaaa accagcattc
     5581 actgaccgga aactgtccgg caggtgctgt ttgtcttacg aagttaatct ccttaaccag
     5641 taatttcccc ggcgtggtgt tgtccttcca taaaacaaac agagccatca gggccattgt
     5701 ggccagtggc agaatgacgg tgtacttcag acaccagaac agtcctcgca gaacccggac
     5761 gacaacatac cgggcggcat ggcacgcctg acgggacgac atattatttg ccatcgctgc
     5821 cccctccctg gctgccggac ataaactgct gcggattcag gttgcccagc tgcttcagca
     5881 tctggtcagt attaaagccg ttaagatgac tttcagccga tggcggaaca ggaaccggac
     5941 tttccggatt gtcttcttcc gctctgctct gcgtgcgttc cagcgccatc tcttccacgg
     6001 ttttcagctg gaagccgtcc tggaacacca cggtcacttc gttgcccgca ccaatcggta
     6061 tcaccgggtg atactgttcg gcacgtttga tgtagtagtc actgagcgtc tgtgcggctt
     6121 tcgatgcgcc gccaccgata cccattttca ggacatcacc agccccgtaa gcggctgtgg
     6181 cacccagccc gacagccggc tgggaggcgc gctccattcc ctgaccgata ccgtcaacaa
     6241 atcccgcgcc ccatgcccag ccgaggattt tgccgttacg catcaccact tcgcccttga
     6301 taccgttttt accccggaag ctgacatgcc ccttaatcgg catatcaata gttttgccgt
     6361 ccttcaggca actgatattg cgggtgcgta caatggcacg ctcactggac acatcccccc
     6421 aggcttccag ccccacaaaa catcccgttg cgtcatacgt cttgctgttc ggcatttcca
     6481 ccaggccggt gatacgcaac tgcatcggca ccgtggattc attaccggtg actgaggcat
     6541 tggcatccgc cccttcaatc agcatggctt tcgcaaaact tcctgacgga atgtacggca
     6601 gcgatggtcc ctgttttccc tcattacggg taaacacctt acgctgtatc cggttaggca
     6661 ccggcacaga ctggtacgtc acctgtggtg gtggcgtgac accattcccg ggataaaacg
     6721 ccgtcggcgg tggaacagca acgctgccct gcggcgggaa ggatacgggg gtgtttcctg
     6781 gctgtggttc gccttccggc ccgggcggtg aggcaggcat ctgaggtaca ggctcaccgg
     6841 tcgccgtgac gggattagca cccagggctt ttacctgctc tgccagggcg gcattgtcct
     6901 gtcccagctt ttcaatacga cgctggtcat caccgcgctg tttgttcagg acatccagtt
     6961 cacgacggat ttcctcatac tgcttctgca tctgcgctgc cgtcacctgc atctctgtgg
     7021 tggcatgctg acgcaccttg tcatcaaagg tcgtatccac cacacctgtc atatccggca
     7081 caggctcctg ttcagccacc gcctcaccgt taccggacat gtccacatca gacagataca
     7141 gtaccccacc aatcgcggag gctgcaccga caaccacaat ccccagccac aggtactgct
     7201 tgcgtttcac aatggtattg atactggcca tcagttgccc tccccgtcac ggatgacata
     7261 cacatccatg cgcgcacctg ccagtaactg gctggcaggc tggctgaaca tcacggcacg
     7321 ggttcccggc tgccagaaat cactttcccg gatattcagg gcagacagcg ttttattttc
     7381 cacggcaaag cggaccatct tcaggtgatt ccccgtccat acggcatctg ccactgaaga
     7441 cagccccgcc ggggcctgca gggtttcctt tgtcacagga acctgatacc agcctgcagg
     7501 taattttccg ccacggacag cctggctgat ggtcacaagc agggattcgt aaggcgtgga
     7561 cgtttcccac gctccggctt cttctccggt tccgcgcagg tcactgacca gctgaatggt
     7621 acgccccgcg ccttcacggg gaacagcctg aatggaaagg ttcagaccac gctctgtttc
     7681 cagaatgaac gtaaagggct ttttgttgac ggtggccacc accacaccgc cggaggcggt
     7741 ctgctcatta ttggtcaggg caccatccag actgttcacg gcgataatcc ggtcaccggg
     7801 aacggtaaac agattcgggc tggtattgct gatggcaata ctggcctgac caccgttcac
     7861 cattggcacc acagtggggg ccagcgtacc gtttgcggcc atcacgctgc aggaaaaaaa
     7921 caggccgccg aatattattg ccgtattatt ttttctcatc gtctgtttcc ccgaaattat
     7981 ccagccaggt caccccgttt tcccgcttca gaataaggat gtaatgtttg atatcagtaa
     8041 aaggttcgga gttaccaatc catgttttcc gaattccacg gatttccaca cgaccatact
     8101 gaggccagac acggacactg gtctggaaaa aagcagaatt cacgttatct tttttaatga
     8161 gtttcgcttc ttcagccaga ataactttca tctggttctg tgcacccggg cggatatatt
     8221 gcagaagcgc ctgatgtgaa gcatcgacga tttccggtga gacattcaga cggagggcaa
     8281 taaatgacag cgccatctgc tgcaaataag aggcatcggc actgtt
//
LOCUS       DAZPDU010000180         8275 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZPDU010000180 DAZPDU010000000
VERSION     DAZPDU010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8275
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.182"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            122..445
                     /gene="soxS"
                     /locus_tag="ACHURS_003719"
     CDS             122..445
                     /gene="soxS"
                     /locus_tag="ACHURS_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFR1239349.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(448..2034)
                     /gene="pdeC"
                     /locus_tag="ACHURS_003720"
     CDS             complement(448..2034)
                     /gene="pdeC"
                     /locus_tag="ACHURS_003720"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFR1239350.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVEKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            2464..2744
                     /locus_tag="ACHURS_003721"
                     /pseudo
     CDS             2464..2744
                     /locus_tag="ACHURS_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
     gene            complement(2843..3379)
                     /gene="ssb1"
                     /locus_tag="ACHURS_003722"
     CDS             complement(2843..3379)
                     /gene="ssb1"
                     /locus_tag="ACHURS_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFR1239351.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            3633..6455
                     /gene="uvrA"
                     /locus_tag="ACHURS_003723"
     CDS             3633..6455
                     /gene="uvrA"
                     /locus_tag="ACHURS_003723"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFR1239352.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(6490..6846)
                     /locus_tag="ACHURS_003724"
     CDS             complement(6490..6846)
                     /locus_tag="ACHURS_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFR1239353.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(6850..7266)
                     /locus_tag="ACHURS_003725"
     CDS             complement(6850..7266)
                     /locus_tag="ACHURS_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFR1239354.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PLHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(7377..8090)
                     /gene="aphA"
                     /locus_tag="ACHURS_003726"
     CDS             complement(7377..8090)
                     /gene="aphA"
                     /locus_tag="ACHURS_003726"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFR1239355.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         2060 a         2094 c         2055 g         2066 t
ORIGIN      
        1 tagcagcgct ttaatgcggg gtaatttctt ttccataaat cgctttacct caagttaact
       61 tgaggaatta tactccccaa cagatgaatt aacgaactga acactgaaaa gaggcagatt
      121 tatgtcccat cagaaaatta ttcaggatct tatcgcatgg attgacgagc atattgacca
      181 gccgcttaac attgatgtag tcgcaaaaaa atcaggctat tcaaagtggt acttgcaacg
      241 aatgttccgc acggtgacgc atcagacgct tggcgattac attcgccaac gtcgcctgtt
      301 actggccgcc gttgagttgc gcaccaccga gcgtccgatt tttgatatcg caatggacct
      361 gggttatgtc tcgcagcaga ccttctcccg cgttttccgt cggcagtttg atcgcactcc
      421 cagcgattat cgccaccgcc tgtagtttta ttgcccgcgc gttaactccc gcgcgggtaa
      481 ttgctccatc cattgcataa acacctgcgg cggcatcgcc ttcgcaaaaa accatccctg
      541 gcaatagcgc acgccgcgtt tgcgcagcca gttaacctgc tcctcagttt cgacgccttc
      601 agcgatcgtt tttaacccca ggctgtgcgc cagctcgatg atgtgttccg caatcaaatg
      661 actggttttg tgggtggtca gcgtttcaac aaacgattta tcgattttca aaatatcgac
      721 attcaatgat ttaaggttat gcaagttaga gtagccaata ccaaaatcat caattgccac
      781 ttcgtaacca gcctggcgga aagccagaat aatcggcgtc attttttcga catcaagaaa
      841 tgcatgctca gtcacttcaa atttaatttg ctgcggacgc accgcgtatt gctctgtttt
      901 ctgattgatt cgcgctatca accgtgacgt atgaaaatcg gaggccgaca ggttaataga
      961 aacatagcga tcggcatgtg ttgccaagta cgcgcccaga tcgcggaaga cattatcaat
     1021 aacataatca gttatctgtt ctatcatccc ctccttttct gccagcggaa taaactctgc
     1081 cggattcatt atttgcccct gctcaccaag ccaacgtaac aacgcttcag cgccgataca
     1141 tttttctgtt ttgatatcga ttattggctg gtaataaaga caaagttgat gtttttcgag
     1201 ggcgcgttgc aatttacgtt tgggagataa atagttttgt cgaatacgta gccagagtaa
     1261 tagcagaaca agactcccca aaataccggc gggcaacgca aaaataagat gattataaaa
     1321 atgggttata agacgttgat atgaagtcgc aacaatggct gcaattgggc gttttgtcga
     1381 ataaactgtc gcatataaat agccatttct ttgtacggtt aaatccttca aatgaatcag
     1441 cggagaaaac gttgctgccg aggcctcttt gcttaacgag aaaaaggttt tcgtcaccgt
     1501 atcatacaca ccccattgca atgtcgggtc atcagacatc acttcactcc agaagagagg
     1561 gttgataacc gccacataat ttccccgctg catataggtc attttatagc cagagaaaaa
     1621 aggcgtatcg cggtaataat agatagaaac gttaggttca cgcttataat cggccggtgc
     1681 aatcgtatag ccgtttacag gcgctatcag cgatgagcat aaaaaatggt tatcacgggc
     1741 atagatcaat tcattaatat aaagatagcc acgaataata ttcaacattc gcttttgatg
     1801 ggctggagtg caaacttgcc cctgatagcg ttcagctgca tcgctcacta aatctgcctg
     1861 gcgaatgacc agctcggatt tatccagtgc cagttgagca aaggttcgga gctgattatt
     1921 cacttctgat tttgcccaaa ggaaggcaat ccatagcgaa agaatgatgg gaaagagaac
     1981 taaaaagata atgcccggca acgccagtaa ttggtgtcgt gcacgatgac tcattgctat
     2041 caatttcctg cctgtttata cccggccatt gcaaggtgaa aaccctgggt accgacgtct
     2101 ttagataaat cttacggtat aaatagcact tttgcctgat ttgaaattga tacctttcta
     2161 atcgtcacct ggatgaaaat aattcattga tattcaaaat taatttccta caggaatgca
     2221 gaggcggcgg gaagatatgc ccttaatgcc actgaaaaac cacataatct gtacagcaat
     2281 aaaagtcacg gcctaatatg gctaacaaac aatcaattgt gatatagctc acaaaattaa
     2341 tgaaacaaac agagtgtttc atttttgtgt tctgcaagcc ccgcgatagc cagagtgtac
     2401 cacgcctccc gtgaacaacg ccgcgctgtc cagggcatcg gctcttttca gaggaattgt
     2461 ttgatggcta ccctcaccac tggcgtggtt cttcttcgct ggcagcttct tagtgccgta
     2521 atgatgtttc tggccagcac atcaacatcc gttttcgtcg gtctgattat gtcgggcttg
     2581 cggtgatcag cagcggtctg ggcgttgttt ccgcctgttg gttcgcaatg gggttgcttg
     2641 gcatcacaat ggcggacatc accgctatct ggcacaacat cgagtcggtg atgatagaag
     2701 agatgaatca gacaccgcca caatggccaa tgattttgac ctgatacgta gaagcctcca
     2761 taacggaggc ttctttttta tggctggcag atgctttaat catccacctt aaaacaatat
     2821 aacctattgt tttaatgaca aatcagaacg gaatgtcatc atcaaagtcc atcggcggct
     2881 cgttagacgg cgctgccgga gcggactgct gcgggcgaga ctgcgcgccg ccgctgaact
     2941 gattgccacc ctgcggctgc tgaggctgac cccaaccgcc ctgcggctga ccaccaccga
     3001 tattaccacc tgccggagcg ccaccaccct gacgaccacc cagcatctgc atggtgccgc
     3061 caacgttcac cacgacttct gtggtgtagc gatcctgacc ggattgatcg gtccatttac
     3121 gggtacgcag ctgaccttcg atataaacct gagaaccttt acgcagatat tcgctggcca
     3181 cttctgccag tttgccgaac agcacaacgc ggtgccattc agtctgttct ttcatctcgc
     3241 cggtcgcttt atcacgccag gattcggaag tagccagcgt aatgttggca actgcgccac
     3301 catttggcat gtagcgtact tccgggtcct gacccagatt accaacgaga ataaccttgt
     3361 ttacgcctct gctggccatg ttcgtgtctc ctgaaaaaaa tcgttctgaa taagtgtaaa
     3421 cgcgcgattg taccattacc aatagcgctt ttactatgtt gtgacctcgg ttccgggaaa
     3481 caaacctggc cagacattgt tacacaacac tccggataat gcattccaat actgtatatt
     3541 cattcaggtc aatttgtgtc ataattaacc gtttgtgatc gccggtagca ccatgccacc
     3601 gggcaaaaaa gcgtttaatc cgggaaaggt gaatggataa gatcgaagtt cggggcgccc
     3661 gcacccataa tctcaaaaac atcaacctcg ttatcccccg cgacaagctc attgtcgtga
     3721 ccgggctttc gggttctggc aaatcctcgc tcgctttcga caccttatat gccgaagggc
     3781 agcgccgtta cgttgaatcc ctttccgcct acgcgcggca gtttctgtca ctgatggaaa
     3841 agccggacgt cgatcatatt gaggggcttt ctcctgccat ctcaattgag cagaaatcga
     3901 cgtctcataa cccgcgttct acggtgggga caatcaccga aatccacgac tatttgcgtt
     3961 tgttgttcgc ccgcgtcggc gaaccgcgct gtccggacca cgacgtcccg ctggcggcgc
     4021 aaaccgtcag ccagatggtg gataacgtgc tgtcgcagcc ggaaggcaag cgtctgatgc
     4081 tgctcgcgcc aatcattaaa gagcgcaaag gcgaacacac caaaacgctg gagaacctgg
     4141 caagccaggg ttacatccgt gctcgtattg atggcgaagt ctgcgatctt tccgatccgc
     4201 cgaaactgga actgcaaaag aaacacacta ttgaagtggt ggttgatcgc ttcaaggtgc
     4261 gtgacgatct tacccaacgt cttgcggagt cgtttgaaac cgcgctggag ctttccggtg
     4321 gtacagcggt agtggcggat atggacgacc cgaaagcgga agagctgctg ttctccgcca
     4381 acttcgcctg cccaatttgc ggctacagta tgcgtgaact ggagccgcga ctgttttcgt
     4441 ttaacaaccc ggcaggtgcc tgcccgacct gtgacggcct tggcgtacag caatatttcg
     4501 atcctgaccg cgtgatccaa aatcccgagc tgtcactggc tggcggtgcg atccgtggct
     4561 gggatcgccg caacttctat tatttccaga tgctgaaatc gctggcagat cactataagt
     4621 tcgacgtcga agcgccgtgg ggcagcctga gcgcgaacgt gcataaagtg gtgttgtacg
     4681 gttctggcaa agaaaacatt gaattcaaat acatgaacga tcgtggcgat acctccattc
     4741 gtcgtcatcc gttcgaaggc gtgctgcaca atatggagcg ccgttataaa gagacggaat
     4801 ccagtgcggt acgtgaagaa ttagccaagt ttatcagcaa tcgcccatgc gccagctgcg
     4861 aaggaacgcg tctgcgtcgg gaagcgcgcc acgtgtatgt cgagaatacg ccgctgcctg
     4921 ctatctccga catgagcatc ggtcatgcga tggaattctt caacaatctc aaactcgcag
     4981 gtcagcgggc gaagattgca gaaaaaatcc ttaaagagat cggcgatcgt ctgaaattcc
     5041 tcgttaacgt cggcctgaat tacctgacac tttcccgctc ggcagaaaca ctttccggcg
     5101 gtgaagccca gcgtatccgt ctggcgagcc agattggtgc gggcctggtt ggcgttatgt
     5161 atgtactgga cgagccgtct atcggcctgc accagcgcga taacgagcgc ctgttgggta
     5221 cgcttatcca tctgcgcgat ctcggtaata ccgtgattgt ggtggagcat gacgaagacg
     5281 caattcgcgc cgctgaccat gtgatcgata tcggtcctgg tgcgggtgtt cacggcggtg
     5341 aagtggtcgc agaaggtccg ctggaagcga ttatggcggt gcctgaatcg ttgaccgggc
     5401 agtacatgag cggtaaacgc aagattgaag tgccgaagaa acgcgttccg gcgaatccag
     5461 aaaaagtgct gaagctgaca ggtgcacgcg gcaacaacct gaaggacgtg acgctcacgc
     5521 tgccagtcgg tctgtttacc tgcatcacag gggtttcagg ttccggtaaa tcgacgctga
     5581 ttaacgacac actgttcccg attgcccaac gccagttgaa tggtgcgacc atcgccgaac
     5641 cggcaccgta tcgcgatatt caggggctgg agcatttcga taaagtgatc gatatcgacc
     5701 aaagcccaat tggtcgtact ccacgttcta acccggcgac ctataccggc gtgtttacac
     5761 ctgtgcgcga actgtttgcg ggcgtaccgg aatcccgtgc gcgtggttat acgccaggac
     5821 gtttcagctt taacgtccgt ggcgggcgct gcgaagcctg tcagggcgac ggtgtgatca
     5881 aagtggagat gcacttcctg ccggatatct acgtgccgtg cgatcagtgc aaaggtaaac
     5941 gctataaccg tgaaacgctg gaaattaagt acaaaggcaa aaccatccac gaagtgctgg
     6001 atatgaccat cgaagaggcg cgtgagttct ttgatgcggt gccagctctg gcgcgtaagc
     6061 tgcaaacgtt gatggacgtt ggtctgacgt acattcgcct ggggcagtcc gcaaccacac
     6121 tttctggtgg tgaagcccag cgcgtgaagc tggcgcgtga gctgtcaaaa cgcggcaccg
     6181 ggcagacgct gtatattctc gacgagccga ccaccggtct gcacttcgcc gatattcagc
     6241 aactcctcga cgtgctgcat aaactgcgcg atcagggcaa taccattgtg gtgattgagc
     6301 acaatctcga cgtgatcaaa accgctgact ggattgtcga cctgggacca gaaggcggca
     6361 gtggcggcgg cgaaatcctc gtctccggta cgccagaaac cgtcgcggag tgcgaagctt
     6421 cgcatacggc acgcttcctc aagccgatgc tgtaatcgtt aaggccgctt tctgagcggc
     6481 cttttccttt cagagttgta ccagcaattt acgcttttct tccggtagta aattcactgc
     6541 ctgctgataa gacgcatcca ccagataata gatttgcgaa tccggcagcg aaccgtcgag
     6601 atacacggtg ctccagtgcg ctttattcag atggcggcta ggacgcacat cgctgtgctg
     6661 ctgacgtagc agctccgcca gctccgggct ggttttcagc gaaacagctg ggcgattttc
     6721 tacttctttc accatcgcaa acagtacatc ttccactttg atctgcgtcg ctttccagtc
     6781 gttatgcacg ctctgttctg cgcctggttt tgccatgcaa tattgtagca actccgaaat
     6841 ggtcattttt tactcccctt gtagtgtcgc gatgatgcga cgcgatccgc cgtggatgcg
     6901 atgttccccc agccagatgc cttgccaggt gccggtctga atacgccctt tatgtaacgg
     6961 caatacaagc gatgttccca gcattgagga tttgatatga gaaggcatat cgtctgctcc
     7021 ctcatagtca tgctcataat ttccgttgtc gggaacggtg cggaggaaaa aacgctccat
     7081 gtcgtggcgt acggtgggat cgcagttctc attaagtgtc agagaggcgg aggtatgttg
     7141 cagcaacaga tgcagtaagc cgatgttaac gcgcggcata tcagccagct gattcagaat
     7201 ttcatccgtt accagatgaa acccacgaga tttggcgcta agcgtgagcg tcttttgata
     7261 ccacatgtgc tgctccttga taaaactctc ttaatcagtt tgcagcaaga cagcgaaagg
     7321 ataaaggtgt gattaaaaaa acagcattcg ggagagcatc acgctctccc gctctgtcag
     7381 tattctgaat tgacgatcac ctcttcacca aacgcacccg cttgtggcaa gggtttgtag
     7441 gtagagttgg aggcgcgcag aatgcggata ccacgagcgc cgacatcgcg tgcggcggta
     7501 atatcgttat cggaatcgcc ataaaaaatt cggatatttt tatcctgcag ccattgcgat
     7561 tttgtatttt gccctggttt atcgcccgca aagattaccg gattcatgtt ggtggcagga
     7621 atatgaaaat tatccgccag cgtttttgaa accgtttctg ttttcgtcgg gctacgacca
     7681 gtcacaaaga agatcgcgtc accgcggcgt acatgcatat caatcagctg gcgagcgacc
     7741 tcttttggaa tgctgaattc atcccagcca ttattcattt tttcccagaa cacaggattt
     7801 ttcagataat cttcgctttc tggcgagaag gtttttttgc cgcgccagaa ccccggactg
     7861 gaaaaaagta ccgtgtcatc gatatcaaac cccaccgcca ttggcggacg ccctgcgagg
     7921 ctattttcaa tttgtgcgac cgaaacccaa tgaattggtg cctgttcagc aagcctggca
     7981 acgttagtcc cagggttaag cggtgaagga gatgaggcca gggcaacagc ggaactgttt
     8041 agagcgaaca ataagcaaac ggcactgatt gcctgtgtga tcttgcgcat atttttccct
     8101 aaatagtcag tatgttgaaa ctttttattg tgagatttgt tgcaaaaacc atctgaccat
     8161 aacgccagct gccggggaaa ggaaggatta tctgcggttt ttgtgatgaa aagcaggatg
     8221 attatattta acccagtaaa tacgttcctg tctttttttg tataaaaaat gttta
//
LOCUS       DAZPDU010000181         8259 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZPDU010000181 DAZPDU010000000
VERSION     DAZPDU010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8259
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.183"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(403..1599)
                     /gene="hemY"
                     /locus_tag="ACHURS_003727"
     CDS             complement(403..1599)
                     /gene="hemY"
                     /locus_tag="ACHURS_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFR1239356.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1602..2843)
                     /gene="hemX"
                     /locus_tag="ACHURS_003728"
     CDS             complement(1602..2843)
                     /gene="hemX"
                     /locus_tag="ACHURS_003728"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFR1239357.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAAAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQATQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPAPAPAPQGDTPAAAPQGE"
     gene            complement(2865..3605)
                     /gene="hemD"
                     /locus_tag="ACHURS_003729"
     CDS             complement(2865..3605)
                     /gene="hemD"
                     /locus_tag="ACHURS_003729"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFR1239358.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGQQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPNYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNAANDALLRALQ"
     gene            complement(3602..4543)
                     /gene="hemC"
                     /locus_tag="ACHURS_003730"
     CDS             complement(3602..4543)
                     /gene="hemC"
                     /locus_tag="ACHURS_003730"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFR1239359.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAKQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4930..7476
                     /gene="cyaA"
                     /locus_tag="ACHURS_003731"
     CDS             4930..7476
                     /gene="cyaA"
                     /locus_tag="ACHURS_003731"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFR1239360.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(7516..7836)
                     /gene="cyaY"
                     /locus_tag="ACHURS_003732"
     CDS             complement(7516..7836)
                     /gene="cyaY"
                     /locus_tag="ACHURS_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFR1239361.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7998..8140)
                     /locus_tag="ACHURS_003733"
                     /pseudo
     CDS             complement(7998..8140)
                     /locus_tag="ACHURS_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
BASE COUNT         1842 a         2176 c         2153 g         2088 t
ORIGIN      
        1 aattgaatga attttatcat tcataataag tatgtgtagg atcaagctca ggttaaatat
       61 tcactcagga agcaaagagg attacagaat tatctcataa caagtgttaa gggatgttat
      121 ttcccgattc tctgtggcat aataaacggg tagatgctca ttccatctct tatgttcgcc
      181 ttagtgcctc ataaactccg gaatgacgca gagccgttta cggtgcttat cgtccactga
      241 cagatgtcgc ttatgcctca tcagacacca tggacacaac gttgagtgaa gcacccactg
      301 ttgtcataca gacctgtttt aacgcctgct ccgtaataag agcaggcgtt tttttatgca
      361 tcagaaaggt cccggaggtg cttgcctccg ggtgagaagg aactactgtg gtgggttgtt
      421 ctgcaacgtt aacatcaaac cgtcgcgacg catcgctgca gcttcttccg gcttgtgcag
      481 tctgtccagc gcgtcggcaa gccatgcgta atcgtaggcg tccggacgtt gtttcagcgc
      541 tgcgcggaag gcgagcgatg cttcctgcca ttctccgtgc ttcatcagtg actggcccag
      601 tgtgctccac aacagcgggc gatcgccgac gtttttgatc tgctggcgca gcactttttc
      661 cagctgttcc ggattgttgg ttttcagtcg aggaatcggc agcagtaggc gatcgtcgta
      721 ctggcgtttc aggccatcga tgataatttg ctgggcagta tcatgatcgt cacattcaat
      781 aagatgttcc gccattgcca cctgcaacgc tacctgatga cgcgttttcc ggctttggtt
      841 tttccaccag ttacgcaaac cttcactacc gttatcggca cgcgcctgat ccatcaggcc
      901 aatccatgcc tgttgttcca gcattgcacg atgttcttca tcaccaacat gggctttcgc
      961 cattgatggg atgatatcca gcagagaact ccatgcacct gtgcggatat acgcctgttc
     1021 cgccagacgt aatacttccg gatggcgtgg cgtaacttcc agcagcttat ccacgccgtg
     1081 gcgtgcagca tggttttcat tacgggccag ttgcagacgt acacgggtga tttctaccgg
     1141 aatggtgtca ttgccggcca gctctgctgc gcgttccaga tgttggttgg cgcgtgcttc
     1201 atcaccacgt tgttgcgcgg cttcggcagc cagtagatag ttcaccaccg gttgttccgc
     1261 gtgatcggca tttttcgcca tcagcttttc aacttgctga taatcgcctt ccgccagttt
     1321 cagcagcgcc tgttcggtct gcttacgggc acggcgacgc ttacgtccga caaaccaccc
     1381 acgggtgtgt gcgcctgtgc ggaagatccg ccgcagtagc cactcaatgg caaacagcac
     1441 taccatcgcc agaatcaaaa taatcgccag gcccgtgacg ctggtttcga tattgtagtt
     1501 gtcagtctgg atcagcacgt aaccctgatg accggcaatc atcgggccaa ccactatccc
     1561 cgcaatcagc aaaacaaaga gtaataacac ttttagcatg gttattctcc ttgcggcgcg
     1621 gctgccggag tatcaccttg cggtgcaggt gcaggtgcag gtgcaggtgc aggtgcaggt
     1681 gcaggtgcag gtgcaggtgc aggtgcaggt gcaggtttag cttccgttgt ccccgctgcc
     1741 ggttgtgcca gcaggttacg cacgcgggtc tgcatcagtt tttccagcat cgcctggctt
     1801 tgcagggttt ccggaagatc catcgagata ttttgctggc ttaactggtc cacctcgtcg
     1861 aggaacgctt tggtggtggc atcatcagta tcgtagtaag cacgtaccca ggtggagacg
     1921 ttctccagcg cctggcgata agtctcttcc tgatgacgcg gtacagcttg tgctgcgacc
     1981 agcaggcgag agcgaatatt ttcgcgcaga tagatatcct gatttggcgc taacagcggt
     2041 acggcggtgt catcacgacg gcgaatcgta atgaagttgt ccataaagtt ctgccagctt
     2101 ttttgcagat tgatacgcca ttcgctgatg gaactggaaa gctcttcacc gtctgaatcc
     2161 atcggcgaac catcgctgtc attatcggcc agacgcaggt tatctacctg atttgaaagc
     2221 tgattaagct taaggatgat gccgtcataa tccacctgcg atactgcaga aaggctggcg
     2281 atatcatcgg taattgcccg acgaacggta atcagactcg ggtcattcat atccgccagg
     2341 ctggcgtctg cacttttcag caacgctgca gcggtcgtga cgtcctgatc gctccacagc
     2401 ttccgtccgg cgagtttcac cagaaaatcg gcctgggcca gcagccaggt tttagcatcg
     2461 ctgccggaaa tggtggcgac cttttgctgg acttcatcca gctgttttgc cagcgtttct
     2521 tgctgacgat tcgcctgttc aagctgcgta gcctgttgct taataatgcc ttccagttcg
     2581 gctttttggc tctcctgggc tttttgcaat gccgtcagtt ggttagccag ggcatcgctg
     2641 gtggcggtct gattgacggc ctgttgttta ccccagccat acaaaccgac gcccgccgcc
     2701 agagcaatag cgatagccgc cgcgctgaga atcaatgcgg tattgttctt actctttttt
     2761 tctgttgcga caggttgtga cgtggtgtcc acggcctccc tagtctcttc aaccacggcg
     2821 gaggtttttt cttgttccgt cattatggct tcctgttatg agagttattg taatgcccgt
     2881 aaaagcgcat cgttggcagc gttatcggcg accttaatgt cttgccagcc cagttcccgg
     2941 gcgagtttcg ccaaacgctc actgacgacc aatagtcgac agtgtagtaa ccagtgctca
     3001 cgataccatt gtgggatcag cgaccagagt tgctgcaaca tttcaccgct ggtaacaacg
     3061 accgtcgtca cctcgcggga ttgccagcgc atcgcttctt ctgcaccatc gtaatggatt
     3121 gcgcatcgtt gataacattc acaaaaagtg acctcagcac cgcgcgccgt cagggtatcc
     3181 ccaattagct cacgaccgcc attgccacgt aatatcagcg cacgtttgcc cgcaatattt
     3241 tgtaattcag gtaattgtag caagacttcg ctgatttccc gatcctgcgg gtagagaatc
     3301 ttctgtccgc ttacggtatg tagtgccagt gcggtggtgc gtccaatggc gaaataatta
     3361 ggtagtcggg gccatttacg atcttgctga tgtagctgtg attgggcaaa agcaaccgcg
     3421 tgttgcgaga gggcaaacaa cagatcgctc tcccccagcg ctgccagttg atcagcaagt
     3481 tgcggtaatt gttgacccgg agaaaactca atcagcggaa aatgccaggc cacctgcccc
     3541 agtgtgcgca gacggctcac taactcttct ccagcgggag acgggcgggt gacaaggata
     3601 ctcatgccgg ggcgtctccg ttatagactt cagcgaggat ctcgcgcgcg ccgttattca
     3661 gtagctcttc tgccagcgaa atccccattt gtttggcatt ttgcggcgca ccgcggcgtt
     3721 caccgcgaat aatctgcgaa ccgtccggcg cgccgaccag cgcacgcagc cagatttcgc
     3781 catcaataag ctcggcgtag ctaccaattg gcacctgaca tccgccttcg agacgggtat
     3841 tcatggcgcg ttctgcggta acgcgcagtg cagtttcgtg gtgattcagc gcggcaagca
     3901 gctcgcgagt gcgcgtatca tcaaggcggc attcaatacc gactgcacct tgtcctaccg
     3961 ccggaagaga aatctcgggt ggtaacgcgg cgcgaatgcg ggactccaga cctaaacgtt
     4021 ttagtccggc tacggcaaga atgatggcat cgtattcgcc gttatctagt ttgctcaggc
     4081 gagtgccgac gttgccgcgc agggagcgga taatcagatc cgggcggcgt tcagccagtt
     4141 ggcactggcg acgtaaactg gacgtcccga cgatactgcc tgctggtaac gcatccagac
     4201 tgtcatagtt attggacaca aaggcatcgc gaggatcttc acgctcacaa atagtgacca
     4261 gtcccagacc ttgcgggaat tcaaccggca catctttcat tgaatgtacg gcgatatcgg
     4321 cgcgattttc gaggagcgcg acttccagct cttttacaaa taagcctttt ccgcctactt
     4381 tcgccagcgg cgtatcaaga atcacatcgc cgcgcgtcac catcggtacc agttcaacga
     4441 ccaggcccgg atggctcgcc atcaacttgt ctttgacata gtgtgcctgc cagagtgcaa
     4501 gtgggctttg gcgtgtggca attcttaaaa cattgtctaa catgcttgtt accgtcatta
     4561 tcatccgtgg tccatcctaa catccttgcc agagtgatgt cagtgttgtg gtgaaacgta
     4621 gacgcctgcg caaaccgtaa aatgaggtct ggcagtggat cctgacagac gtttcacgct
     4681 gtcgtaataa ggaatttaca gagaataaac ggtgctacac ttgtatgtag cgcatctttc
     4741 tttacggtca atcagcaagg tgttaaattg atcacgtttt agaccatttt ttcgtcgtga
     4801 aactaaaaaa accaggcgcg aaaagtggta acggttacct ttgacatacg aaatatcccg
     4861 aatgccgcgt gttaccgttg atgttggcgg aatcacagtc atgacgggta gcaaatcagg
     4921 cgatacgtct tgtacctcta tattgagact ctgaaacaga gactggatgc cataaatcaa
     4981 ttgcgtgtgg atcgcgcgct tgctgctatg gggcctgcat tccaacaggt ctacagtcta
     5041 ctgccgacat tgttgcacta tcaccatccg ctaatgccgg gttaccttga tggtaacgtt
     5101 cccaaaggca tttgccttta cacgcctgat gaaactcaac gccactacct gaacgagctt
     5161 gaactgtatc gtggaatgtc agtacaggat ccaccgaaag gtgagcttcc aattactggt
     5221 gtatacacca tgggcagcac ctcgtccgta gggcaaagtt gttcctctga cctggatatc
     5281 tgggtctgtc atcaatcctg gctcgatagc gaagagcgcc agttactaca acgtaaatgc
     5341 agcctgctgg aaagctgggc cgcctcgctg ggtgtggaag tcagcttctt cctgattgat
     5401 gaaaaccgct ttcgtcataa tgaaagcggc agcctggggg gcgaagattg tggctccacc
     5461 cagcatatac tgctgcttga cgaattttat cgtaccgccg tgcgtctcgc cggtaagcgt
     5521 attctgtgga atatggtgcc gtgcgacgaa gaagagcatt acgacgacta tgtgatgacg
     5581 ctttacgcgc agggcgtgct gacgccaaat gaatggctgg atctcggtgg cttaagctcg
     5641 ctttctgctg aagagtactt tggtgccagc ctttggcagc tctacaagag tatcgattcc
     5701 ccatacaaag cggtgctgaa aacactgctg ctggaagcct attcctggga atacccgaac
     5761 ccacgtctgc tggcgaaaga tatcaaacag cgtttgcacg acggcgagat tgtatcgttt
     5821 ggcctcgatc catactgcat gatgctggag cgtgttactg aatacctgac ggcgattgaa
     5881 gatttcaccc gtctggattt agtacgtcgc tgcttctatt taaaagtgtg cgaaaaactc
     5941 agccgtgaac gcgcctgtgt aggctggcgt cgcgcagtgt tgagccagtt agtgagcgag
     6001 tggggttggg acgaagctcg tctggcaatg ctcgataacc gtgctaactg gaagattgat
     6061 caggtgcgtg aggcgcacaa cgagttgctc gacgcgatga tgcagagcta ccgtaatctg
     6121 atccgctttg cgcgtcgcaa taaccttagc gtctccgcca gtccgcagga tatcggcgtg
     6181 ctgacgcgta agctgtatgc cgcgtttgaa gcattaccag gtaaagtgac gctggtaaac
     6241 ccgcagattt cacccgatct ctcggaaccg aatctgacct ttatttatgt gccgccgggc
     6301 cgggctaacc gttcaggttg gtatctgtat aaccgcgcgc caaatattga gtcgatcatc
     6361 agccatcagc cgctggaata taaccgttac ctgaataaac tggtggcgtg ggcatggttt
     6421 aacggtctgc tgacctcgcg cacccgtttg tatattaaag gtaacggcat tgtcgatttg
     6481 cctaagttgc aggagatggt cgccgacgtg tcgcaccatt tcccgctgcg cttacctgca
     6541 ccgacaccga aggcgctcta cagcccgtgt gagatccgcc atctggcgat tatcgttaac
     6601 ctggaatatg acccgacagc ggcgttccgc aatcaggtgg tgcattttga tttccgtaag
     6661 ctggatgtct tcagctttgg cgagaatcaa aattgcctgg taggtagcgt tgacctgctg
     6721 taccgcaact cgtggaacga agtgcgtacg ctgcacttca acggcgagca atcgatgatc
     6781 gaagccctga aaactattct cggcaaaatg catcaggacg ccgcaccgcc agatagcgtg
     6841 gaagtcttct gttatagcca gcatctgcgc gggttaatcc gtactcgcgt gcagcaactg
     6901 gtttctgagt gtattgaact gcgcctttcc agcacccgcc aggaaaccgg gcgtttcaag
     6961 gcgctgcgcg tttctggtca aacctggggc ttgttcttcg aacgcctgaa tgtatcggta
     7021 cagaagctgg agaacgccat cgagttttat ggcgctattt cgcataacaa actgcacggc
     7081 ctgtcagtgc aggttgaaac caatcacgtc aaattaccgg cggtggtgga cggctttgct
     7141 agtgaaggga tcatccagtt cttcttcgaa gaaacgcaag acgagaatgg ctttaatatc
     7201 tacattcttg acgaaagcaa ccgggttgag gtgtatcacc actgcgaagg cagcaaagag
     7261 gaactggtgc gtgacgtcag tcgcttctac tcgtcatcgc acgaccgctt cacctacggc
     7321 tcaagcttca tcaacttcaa cctgccgcag ttctatcaga ttgtgaaggt tgatggtcgt
     7381 gaacaggtga ttccgttccg cacaaaatct atcggtaaca tgccgcctgc caatcaggat
     7441 cacgatacgc cgctattaca gcaatatttt tcgtgatgaa cgtgccggaa agcgaggctt
     7501 atccggcatg caatcttagc ggaaactgac tgtttcaccc gcctgctgcg tcgccgcctg
     7561 ttccagcaaa tcccagaagg tttcgccgct gcgatcgcaa atccactcat cgcctttcag
     7621 atcaaaatgg tagccgcctt gtttggttgc cagccatacc tggtgcagcg gttcctggcg
     7681 gttgataatg attttgctgc cgttttcaaa ggtaatggtc agcacgccgc cgttgatttc
     7741 gcagtcgata tcgctgtcgc catcccagtc gtccaggcgt tcttcaatgg tcagccagag
     7801 ttgatcagcc aggcgatgaa attcactgtc gttcattgtt gtatcctgtt tttaagtgat
     7861 ggcggcagta tagcggcatg gggtcagggc ttcaaagttt gcacctctgc ggctgcgttc
     7921 cggcacgatt catccgtcac cggaataatg atgtctctgt gtagcgaaag atttgtctct
     7981 tcattagggc gcagttacac cacgcctttc cctgtttctg gtaaacatta tgatcaggtt
     8041 taccgagcga gcatcctcac gctgacggaa ctaaaaaaga caacaaacaa accacattgc
     8101 gatagtgcat agagccatcc tggcgcgagg tgccgatcac gaaactacca gcaaaacata
     8161 aatccccacg agtaagcgtt atactcgcag catttcctca cttttcagac ttcataaaga
     8221 gtcgctaaac gcttgctttt acgtcttctc ctgcgatga
//
LOCUS       DAZPDU010000182         8215 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZPDU010000182 DAZPDU010000000
VERSION     DAZPDU010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8215
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.184"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..100
                     /gene="mgtT"
                     /locus_tag="ACHURS_003734"
     CDS             <1..100
                     /gene="mgtT"
                     /locus_tag="ACHURS_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFR1239362.1"
                     /translation="GDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(215..1105)
                     /gene="dgcZ"
                     /locus_tag="ACHURS_003735"
     CDS             complement(215..1105)
                     /gene="dgcZ"
                     /locus_tag="ACHURS_003735"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFR1239363.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTDPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(1360..1753)
                     /gene="ydeI"
                     /locus_tag="ACHURS_003736"
                     /pseudo
     CDS             complement(1360..1753)
                     /gene="ydeI"
                     /locus_tag="ACHURS_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
     gene            2029..2547
                     /locus_tag="ACHURS_003737"
     CDS             2029..2547
                     /locus_tag="ACHURS_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFR1239364.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            complement(2592..4637)
                     /gene="dcp"
                     /locus_tag="ACHURS_003738"
     CDS             complement(2592..4637)
                     /gene="dcp"
                     /locus_tag="ACHURS_003738"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFR1239365.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            4774..5520
                     /gene="ydfG"
                     /locus_tag="ACHURS_003739"
     CDS             4774..5520
                     /gene="ydfG"
                     /locus_tag="ACHURS_003739"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFR1239366.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            5609..6295
                     /gene="rspR"
                     /locus_tag="ACHURS_003740"
                     /gene_synonym="ydfH"
     CDS             5609..6295
                     /gene="rspR"
                     /locus_tag="ACHURS_003740"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFR1239367.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            6472..6675
                     /gene="ydfZ"
                     /locus_tag="ACHURS_003741"
     CDS             6472..6675
                     /gene="ydfZ"
                     /locus_tag="ACHURS_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFR1239368.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(6711..8171)
                     /locus_tag="ACHURS_003742"
     CDS             complement(6711..8171)
                     /locus_tag="ACHURS_003742"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFR1239369.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIIHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
BASE COUNT         2170 a         2056 c         1871 g         2118 t
ORIGIN      
        1 cggagataat ccctcaccta accggcccct tgttacagtt gtgtacaagg ggcctgattt
       61 ttatgacggc gaaaaaaaac cgccagtaaa ccggcggtga atgcttgcat ggatagattt
      121 gtgttttgct tttacgctaa caggcatttt cctgcactga taacggatcg ttgacacagt
      181 agcatcagtt ttctcaatga atgttaaacg gagcttaaac tcggttaatc acattttgtt
      241 cgtcaataaa catgcagcga tttcttccgg tttgcttacc ctcatacatt gcccggtctg
      301 ctcttccaat gaccacatcc agaggctctt caggaaatgc gcgactcaca cctgctgtca
      361 cggtaatgtt gatatgccct tcagaatgtg tgatggcatg gttatcgact aactggcaaa
      421 ttctgacacc tgcacgacat gcttcttcat cattagccgc tttaacaata atgataaatt
      481 cttcgccccc gtagcgataa accgtttcgt aatcacgcgt ccaactggct aagtaagttg
      541 ccagggtgcg taatactaca tcgccgatta aatgcccgta ggtatcatta accaatttaa
      601 atcggtcaat atccaacaac attaaataaa gattcagagg atcagtgttg cgtaactgat
      661 gatcaaagga ttcatcaaga acccgacgcc ccggcaatcc cgtcaaaaca tccatattgc
      721 tacggatcgt cagcaaataa attttgtaat cggttaatgc cgcagtaaaa gaaagcaacc
      781 cctcctgaaa ggcgtcgaaa tgcgcgtcct gccagtgatt ttcaacaata gccagcatta
      841 attcccgacc acagttatgc atatgttgat gggcagaatc cattagccga acgtaaggta
      901 attcatcgtt atcgagtggc cccagatgat caatccaccg accaaactgg cacagtccat
      961 aagaatggtt atccgttatt tctggcttac tggcatctct cgcgaccacg ctgtgaaaca
     1021 tactcaccag ccactggtag tgggcatcga tagccttatt gagatttaac aagatggcat
     1081 caatttccgt tgtcttcttg atcattgcca ctcctttttc acagttcctt gtgcgcgcta
     1141 ttctaacgag agaaaagcaa aattacgtca atattttcat agaaatccga agttatgagt
     1201 catctctgag ataacattgt gatttaaaac aaaatcagcg gataaaaaag tgtttaattc
     1261 tgtaaatcac ctctgcatta tcgtaaataa aaggatgaca aatagcataa cccaataccc
     1321 taatggccca gtagttcagg ccatcaggct aatttatttt tatttctgca aatgagtgac
     1381 ccgaacgacg gccggcgcgc ttttcttatc cagactgccg ctaatgttga tcatctggtc
     1441 cggctgaact tctcgtccat caaagacggc cgcaggaata acaacattaa tttcaccgct
     1501 cttatcgcga aaaacgtaac ggtcctctcc tttgtgagaa atcaaattac cgcgtagtga
     1561 aaccgaagcg ccatcgtgca tgttttttgc gaaatcaacg gtcatttttt ttgcatcatc
     1621 ggttccgcga tagccatctt ctattgcatg aggcggcggt ggcgctgcca tcctgtttta
     1681 aaccgccctg gtcatctgcc aacgcataag gcatgacaag aaaacttgct aatacaatgg
     1741 actgaaattt catactaact ccttaattgc gtttggtttg acttattaag tctggttgct
     1801 atttttataa ttgccaaata agaatactgc caattgttat aaggcattta aaatcagccc
     1861 actagctgtc aaatattcag agaatttgac tcactaaagt taagaagatt gaagagtctt
     1921 aaacatgttt tcagaataat cggatttata tgtttgaaaa ttattatatt ggacgagcat
     1981 acagaaaaag caaatcacct ttacatataa tagcgtggac aaaaaacagt gaacattaat
     2041 aaagataaaa ttgtacaact cgcagatacc gacactattg aaaacctgac atccgcgttg
     2101 agtcaaagac ttatcgcgga tcaattacgc ttaactaccg ccgaatcatg caccggcggt
     2161 aagttggcta gcgccctgtg tgcagctgaa gatacaccca aattttacgg tgcaggcttt
     2221 gttactttca ccgatcaggc aaagatgaaa atcctcagcg taagccagca gtctcttgaa
     2281 cgatattctg cggtgagtga gaaagtggca gcagaaatgg caacaggtgc catagagcgt
     2341 gcggatgctg atgtcagtat tgccattacc ggctacggcg gaccggaggg cggtgaagat
     2401 ggtacgccag cgggtaccgt ctggttcgcg tggcatatta aaggccagaa ctacactgcg
     2461 gttatgcatt ttgctggcga ctgcgaaacg gtattagctt tagcggtgag gtttgccctc
     2521 gcccagctgc tgcaattact gctataacca ggctggcctg gcgatatccc caggccagcc
     2581 attggtggtg tttatatgtt caagccacga tgttgcagca tcggcataat ctgaggagcc
     2641 ttaccgcgcc attgtcgatg caggcgttcc agatcttcgc tgttacctct ggaaaggatc
     2701 gcctcgcgaa aacgctgccc attttcacgc gttaatccgc cctgctcaac aaaccactgg
     2761 taaccatcat cggccaacat ttgcgtccac agataagcgt aataacctgc agcatatccg
     2821 ccaccaaaaa tatgggcgaa ataactgctg cgatagcgtg gcggtatagc aggaagatcc
     2881 atattttccg ccaccagcgc ccgcaattca aaatcatcga catcctgcat tgcttcgttt
     2941 tcttccaggc aatgccagcg catatcgaga agtgcggcgc taagcagttc gctcatctca
     3001 taccctttgt tgaacaggct ggcattacgc attttctgtt gcagttcgtc aggcattgct
     3061 gccccgctct gataatgccg ggcgtagcga gcgaatacct gcggatgcgt tgcccagtgt
     3121 tcgttgattt gcgacggaaa ttcgacaaaa tcacgcggcg tgttggtgcc ggaaagcgtg
     3181 gcataacgct ggcgggcaaa aaggccgtgc agcgtatgac caaattcatg gaataaggtt
     3241 attacatcat cccagagtaa caacgcaggc tcaccggcag cgggtttctg ataattgcag
     3301 acgttataaa ttaccggatg cgtttcatta agcgttgatt gctcaacaaa attgcccatc
     3361 catgcaccgc cgctttttga atcacgggcg aagaaatcac cgtaaaataa cgccagcccc
     3421 acgccattat gatcaaaaat ttcccacacc cgaacgtcag gatggtagac aggaatatca
     3481 aaacgttcga caaacttaat accgaagagc tgattcgcgg tccagaatac accttcattc
     3541 aacaccgtgt ttaattcaaa atatggcttg agctgcgctt catcaagatc aaatttctcc
     3601 cgccgtacct gttccgcata aaatgcccag tcccacggct gcgcgctaaa cccgccctgc
     3661 tgcttatcga taaccgcctg tatggaggct aattcatcgc tcgcacgttg acgcgccgct
     3721 ggaacaattt cccgcataaa gttgagtgct gcttctggcg tttttgccat ctgatcggcg
     3781 attttccatg cggcataatg aggaaaacca agcagtttcg cctgctgtgc gcgaatctct
     3841 accagacgtt gaatgatagc gcgggtatca ttgccatcat ttttttccgc tcgcgtccag
     3901 cccgcaataa acagtttttc acgcgtcgca cgatcgcgca tttcggcaag cgccggttgt
     3961 tgggtggtat tcagcagcgg aatcagccat ttgttatcca gacctttctc gcgagccgcc
     4021 tctgccgcca gcgcaatctc ttgctcactc attcctgcca gctgcgcgat atcgttcaca
     4081 accagaccgc cggatttatt tgctgccagt aatcgctggt taaactggct ggtcagggtc
     4141 gcagcttctg tattcagtac ttttaatttt gctttatcag cttgcgcaag tttggctccg
     4201 gcaaggacaa aacgttgatg aatcacctcc accaggcgga tggattcact atcaagcccc
     4261 agggattcac ggcgctgcca gacagcatct acccgcgcga ataattcacc gttcagatag
     4321 atatcattag ccagttccgc cagttcagcg gaaaactgtt catcaagacg ctgtaattca
     4381 tcattggtat gcgccgcagt catcgcaaaa aagacgctgg taacgcgggt aagtaattct
     4441 ccgctttgtt ccagtgccag aatagtattg ttgaaatcag gcgtttgcgg gttaagcgcg
     4501 atggcagcaa tttctgcccg cttttgctgc attccctcat cgaatgccgg gcgatagtga
     4561 tgattggcaa tttgatcaaa atggggagcc agatacggta gtgtgctttg cacaaggaaa
     4621 ggattcattg ttgtcatttt cttctcctga acgcgagatg ttccatagcg taggcttact
     4681 gataacggag tgcaatcttg caaaccagta ttacccgctc ttaagcatca cgtgctatgt
     4741 tattgacaca caaaagcgtt gaggaacagt gagatgatcg ttttagtaac tggagcaacg
     4801 gcaggttttg gtgaatgcat tactcgtcgt tttattcaac aagggcataa agttatcgcc
     4861 actggccgtc gccaggagcg gttgcaggag ttaaaagacg aactgggaga taatctgtat
     4921 atcgcccaac tggacgttcg caaccgcgcc gctattgaag agatgctggc atcgcttcct
     4981 gccgagtggt gcaatattga tatcctggta aataatgccg gcctggcgtt gggcatggag
     5041 cctgcgcata aagccagcgt tgaagactgg gaaacgatga ttgataccaa caacaaaggc
     5101 ctggtatata tgacgcgcgc cgtcttaccg ggtatggttg aacgtaatca tggtcatatt
     5161 attaacattg gctcaacggc aggtagctgg ccgtatgccg gtggtaacgt ttacggtgcg
     5221 acgaaagcgt ttgttcgtca gtttagcctg aatctgcgta cggacctgca tggtacggcg
     5281 gtgcgcgtca ccgacatcga accgggtctg gtgggtggca ccgagttttc caatgtccgc
     5341 tttaaaggcg atgacggtaa agcggaaaaa acctatcaaa ataccgttgc attgacgcca
     5401 gaagatgtca gcgaagccgt ctggtgggtg tcaacgctgc ctgctcacgt caatatcaat
     5461 accctggaaa tgatgccggt tacccaaagc tatgccggac tgaatgtcca ccgtcagtaa
     5521 tttttatacc cggcgtaact gccgggttat tgcttgtcac aaaaaagtgg tagactcatg
     5581 cagttaactc actcacaagc aagaacgaat gaccgtcgaa acgcaactta atcccacaca
     5641 gcctgtcaat cagcaaattt atcgtattct tcgtcgcgac attgtccatt gcctgattgc
     5701 accaggcaca ccgttgtcgg aaaaagaagt ttctgttcgt ttcaatgtgt cacgccagcc
     5761 ggttcgtgaa gcctttatta aactggcgga aaacggcctg attcaaattc gtccgcagcg
     5821 tggcagctac gtcaacaaaa tttccatggc ccaggtgcgc aatggcagtt ttatccgtca
     5881 ggccattgag tgcgcggtgg cgcgtcgggc ggcgagcatg attaccgaaa gccagtgcta
     5941 ccaactggaa caaaatcttc accagcaacg cattgccatt gagcgcaagc aactggatga
     6001 tttttttgaa cttgatgaca acttccatca actcctgacg cagattgccg actgtcaact
     6061 ggcgtgggat accattgaga acctgaaagc gaccgttgat cgcgtgcgct atatgagttt
     6121 cgaccacgtt tctccaccag aaatgctgtt acgccagcat cttgatattt tctctgccct
     6181 gcaaaaacgt gatggcgatg cggtagaacg tgcaatgacg caacatttgc aggaaatcag
     6241 cgaatccgtg cgccagatcc gccaggaaaa tagcgactgg tttagcgaag agtaattcat
     6301 ttcctctcat cccatccggg gtgagagtct tttcccccgc cttatggctc atgcatgtat
     6361 caaaaaagat gtgagcttga tcaaaaacaa aaaatatttc actcgacagg agtatttata
     6421 ttgcgcccgt tacgtgggct tcgactgtaa atcagaaagg agaaaatacc tatgacgacc
     6481 tacgatcgta accgtaacgc aatcaccact ggcagccgtg ttatggttag cggcaccggt
     6541 cacactggca agatcctgtc gattgatact gaaggtctga ccgctgagca aatccgccgc
     6601 ggaaaaaccg tagttgttga aggttgtgaa gagaaactgg cacctctgga cctgattcgt
     6661 ctcggcatga actaagcgtg tgaatgccgc cgatggcggc attgcttttt ttacttcacg
     6721 gaatatttcg ccacggtcgc tttcgcgcca tgcgctaata aagacaagta cgcttccgtc
     6781 acttttgcag taaacaaact attgtctggc aaatcatcac caaagatcgc cttaatcgcc
     6841 agcaatgact ggacgcgcgc tttcccttca gcactacttt gtacagcctt ctgaataaca
     6901 ggtaacagtg ggtcactgat ttcaatcgga tttccctgtt catcaacacc accgacataa
     6961 cgcatccaac ccgcgacgcc cagtgccagc agatcgaact tgctgtcatg cgccagatgc
     7021 cagcgaacag aatccaacat ccgctgtggc aatttctggc tgccatccat cgcaatctgc
     7081 caggttcgat gacgtaacgc cgggttgcta tagcgttcaa ttaatcggtt agcgtaatct
     7141 tgcaaatcaa cgccctgcac tttcagcgtc ggcgcttgtt cctgcaacat caagccatac
     7201 gccgcataac gataatgttc atcttccatg cagtcattaa tgtgctgata tccagcaaga
     7261 taacccaaat acgccaggaa tgaatgactg ccgttgagca tgcgcaactt catctcttca
     7321 taaggcagca catcactaac cagttcggct cccgcttttt cccattctgg acgtccggca
     7381 acaaagttat cttctattac ccactggcgg aaaggttcac aggcaacgcc cgcaggatcg
     7441 cccacaccgg taagttgttc gattttcgcc agcgtatcct ctgtcactgc gggcacaata
     7501 cggtccacca ttgttgatgg gaaagtcacg ttatcttcga tccattgtgc cagttttaca
     7561 tcaacagctt tcgcgtatga agtgacaacg tcacgcataa catgaccgtt ttctggcatg
     7621 ttgtcacatg acatgacggt aaatgcggga agtcctgccg ctttacggcg ggccagcgcc
     7681 tcaacaatca cccctgttgc tgttttcggc tggtggggat tttgcacgtc ggcagcgacc
     7741 atcgggtgat cgagcattaa ctgtccggtc gccggagagt ggaaataccc tttttcggtg
     7801 attgtcagag agacaatcgc gatttgcggt tcacacatcg cagccaacac ggtttctaag
     7861 ccatccatct gtacgtgcaa ggctttctta acgacgccaa cgacgcgagc cgtccacgca
     7921 tcggccgaca tttccgcaac ggtataaaga ttatcttgct gttgtaaatc ggcaatttgc
     7981 tgttcgccgc cgattaagtt gacctcataa tatccccagt cactgaaatg ttctgtagca
     8041 agaatatcgg catacacgcc ctgatgcgca cggtgaaatg caccaaagcc taaatgaata
     8101 attcttgggg ccaggttatt acgatcataa acagggagtg tcgcttttgc tgataacaaa
     8161 ttatttccca taacaattcc ttaaatataa atatggcaag ctatatgttt tgtta
//
LOCUS       DAZPDU010000183         8207 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZPDU010000183 DAZPDU010000000
VERSION     DAZPDU010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8207
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.185"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            128..739
                     /gene="ruvA"
                     /locus_tag="ACHURS_003743"
     CDS             128..739
                     /gene="ruvA"
                     /locus_tag="ACHURS_003743"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFR1239370.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARTDASSETLIREALRAAL"
     gene            748..1758
                     /gene="ruvB"
                     /locus_tag="ACHURS_003744"
     CDS             748..1758
                     /gene="ruvB"
                     /locus_tag="ACHURS_003744"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFR1239371.1"
                     /translation="MIEADRLISAGTTLPEDGADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(1905..2690)
                     /gene="znuB"
                     /locus_tag="ACHURS_003745"
     CDS             complement(1905..2690)
                     /gene="znuB"
                     /locus_tag="ACHURS_003745"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFR1239372.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(2687..3442)
                     /gene="znuC"
                     /locus_tag="ACHURS_003746"
     CDS             complement(2687..3442)
                     /gene="znuC"
                     /locus_tag="ACHURS_003746"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFR1239373.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            3521..4453
                     /gene="znuA"
                     /locus_tag="ACHURS_003747"
     CDS             3521..4453
                     /gene="znuA"
                     /locus_tag="ACHURS_003747"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFR1239374.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPGAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4469..5791
                     /gene="mepM"
                     /locus_tag="ACHURS_003748"
     CDS             4469..5791
                     /gene="mepM"
                     /locus_tag="ACHURS_003748"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFR1239375.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAAHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            5911..6882
                     /gene="lpxM"
                     /locus_tag="ACHURS_003749"
                     /gene_synonym="msbB"
     CDS             5911..6882
                     /gene="lpxM"
                     /locus_tag="ACHURS_003749"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFR1239376.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(6970..8207)
                     /locus_tag="ACHURS_003750"
                     /pseudo
     CDS             complement(6970..8207)
                     /locus_tag="ACHURS_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
BASE COUNT         2196 a         1951 c         2052 g         2008 t
ORIGIN      
        1 aattatactg tgccattttt cagttcatcg agacacctcg caagttttct tcatccttcg
       61 ctggatatct atccagcatt tttttatcat acagcattat ctttgattca ttacgcagga
      121 gcgtcatgtg ataggcagac tcagaggcat catcattgaa aaacaacccc cgctggtgtt
      181 aattgaagtg ggcggcgtag gctatgaagt gcatatgccg atgacctgtt tttatgaact
      241 ccctgaagcg ggtcaggaag cgatcgtttt cacccacttt gtggtgcgtg aagacgcgca
      301 actgctgtac ggttttaaca ataaacaaga gcgcacgttg tttaaagagt taatcaaaac
      361 caacggcgtc ggcccgaagt tggcgctggc gatcctctcc ggaatgtcag cgcagcagtt
      421 cgttaatgcc gttgaacgtg aagaagtggg agcactggtg aaactgccgg gcattggtaa
      481 aaaaaccgcc gagcgtttaa ttgtcgaaat gaaagaccga tttaaaggtt tgcacggcga
      541 tctctttacg ccagccgccg atctggtgct cacgtcgcct gccagcccgg caaccgacga
      601 tgctgaacag gaagcggttg ccgcgctggt ggcgctgggc tataaaccac aagaagcaag
      661 ccgcatggtg agcaaaatcg ctcgcactga cgccagcagt gaaacattaa ttcgcgaagc
      721 cctacgcgcc gcgttatgag gtaaaggatg attgaagcag accgtctgat ttctgccggt
      781 accactttgc cggaagatgg ggcagatcgc gccattcgcc ctaaattact ggaagagtat
      841 gttggtcagc cgcaggttcg ttcacagatg gagattttca tcaaagcagc gaaactgcgc
      901 ggcgatgccc tcgatcatct gttgattttt ggtcctccgg ggttgggtaa aactacgctt
      961 gccaacattg tcgccaatga aatgggcgtt aatttacgca cgacttctgg tccggtgctg
     1021 gaaaaggcgg gcgatctggc agcgatgctc actaaccttg aaccgcatga cgtgctgttt
     1081 attgatgaga tccaccgtct atcgccagtt gttgaagaag tgctgtaccc ggcaatggaa
     1141 gactaccaac tggatatcat gattggtgaa ggtccggcgg cacgctccat taaaattgat
     1201 ttgccgccgt ttaccctgat tggtgcaacc acgcgcgcag gttcgctgac atcaccgttg
     1261 cgcgaccgtt ttggtattgt gcaacgtctg gagttttatc aggtgccgga tctgcaatat
     1321 atcgtcagtc gcagcgcacg ctttatgggg cttgagatga gtgatgacgg cgcgctggaa
     1381 gttgctcgtc gcgctcgcgg tacgccacgt attgccaacc gtctgctgcg tcgagtgcgt
     1441 gatttcgccg aagtgaagca cgatggcacc atctcggcag atatcgctgc tcaggcgctg
     1501 gatatgttga atgtcgatgc tgaaggtttc gattatatgg accgcaaatt gttgctggcg
     1561 gtaatcgata agttctttgg tgggccggta ggtctggata acctggcggc agccattggc
     1621 gaagaacgtg aaaccattga ggatgtgctg gagccttatc tgattcagca aggctttttg
     1681 cagcgtacac cgcgtgggcg tatggcgacg acgcgggcgt ggaatcactt tggcataacg
     1741 ccgccagaaa tgccgtaagt cggattggtc gctcgcaccg catccgacat tggcagtgtg
     1801 cctgatgcga cgctattcgc gtcttatccg gcctaccaga gatagcgaac cgtaggtcgg
     1861 ataaggcgct cgcgccgcat ccgacaaatg tgttcagcga cagattagct ggcctgcttt
     1921 ttcatcatac tgataataaa taacagtgca gcacatagca ctaccgacgg acccgccggc
     1981 gtatcgtaaa acgcggaaaa ggttaaaccg ccagttactg ccaccatccc caccaaaaca
     2041 gcgacaccag ccatctgttc cggcgtacgg gcaaagcgac gcgcagtagc tgcaggaata
     2101 atcagcaacg aagtaataat caacgcaccg acaaatttca tcgctacacc aatcgtcaat
     2161 gccgtcacca gcatcaacaa caatttcacg cgctgtaatt tcacaccatc aacaaacgcc
     2221 agatccgggc taatcgtcat cgacagcaaa ttgcgccatt gccagaacaa aatagccacc
     2281 acgatgacca cgccaatcgc aatagagatg agatcttctg gcgtcactgc cagcaaatca
     2341 ccgaacaggt aagccatcaa atcaacacga atattagaca tcagactaac gaccaccagg
     2401 cccagcgaca gggcactgtg cgccataatc cctaataacg tgtcgatcgc cagctgtgga
     2461 cgcttctcca gccataccag accgcccgcc agcagcagcg taacggcaat caccgcatag
     2521 aatggattca cgtccagcaa caaaccaaac gcgacaccaa gtaatgaggc atgagccagc
     2581 gtatcaccga aataagacat acgacgccag actacaaacg aacccagcgg acccgcggca
     2641 caggcgagca tgatcccggc taaccaaccg ggaaataata attcaatcat gagcgatcat
     2701 ttccccgacg caaaacaatt cgtccctgta aatcgtgacg atggttatga tgatggcgat
     2761 agatacccag ttgttcagca ccacgaggac caaacattga aataaactcc ggatgcaggg
     2821 aaacaacttc cggtgtgccg gaacaacaaa tgtggtgatt caggcacagc acttcatcgg
     2881 tttttgccat caccagatgc agatcgtgag aaaccattaa aacgccacaa tccagttcac
     2941 gacgcagttg gtcaataagg tcatataacg ccacctggcc atttacatcc acgccttgag
     3001 tgggttcatc cagcactaat aattgcgggc gatttaacaa tgctcgcgct aacagtacac
     3061 gctgcgtttc gccaccagag agcttttgca tcggtgcgtt aatcaggtgc ccggcctgga
     3121 cacgtttcag tgcaggcaaa atatcttctt tatgtgtacc agggcgtaag cgtaaaaaac
     3181 ggtttacggt cagtggcaac gtggtgtcga gatacagctt ctgcggtaca tagccgatgc
     3241 gcagttttcc gttgcgcttg ataacccctt catcgggtgt taccagcccg agcactaccc
     3301 gtaccagtgt cgacttacct gcgccatttg gcccaagtaa agtcaaaatt tttccaggtt
     3361 taagttccag cgacacatca gagaggacgc ggcgttggcc aaaagaaacc gagacatttt
     3421 ccaaggaaac cagacttgtc atgttaattt tagtcttgca gtagtcatga aatgttataa
     3481 tatcacactt ctcatattca ttacgatgat tggtcgcatt atgttacata aaaaaacgct
     3541 tcttttcgca gcattatccg ccgctctctg gggaggtgca acacaggccg cagatgccgc
     3601 cgttgtcgct tcgcttaaac ccgttgggtt catcgcttct gccattgctg atggggtaac
     3661 agaaacagag gttttacttc ctgacggcgc ttcagaacat gattattcac tgcgcccatc
     3721 ggatgtaaaa cgcttacaga acgcggactt agtcgtttgg gttggcccgg agatggaagc
     3781 gtttatgcaa aaaccggtaa gcaaattacc aggagcgaag caggtaacga ttgcgcagct
     3841 tgaagatgtg aaaccgctac tgatgaaaag tattcacggc gatgatgatg atcacgacca
     3901 cgcggaaaaa agtgacgaag atcaccatca cggcgatttc aacatgcatc tttggctttc
     3961 cccagagata gcgcgggcta cagcggttgc aatccatgga aaattagtgg aacttatgcc
     4021 gcaaagtcga gccaaacttg acgccaacct gaaggatttt gaggcacaat tagcctcaac
     4081 cgaaacgcag gttggtaacg agctcgcgcc gctcaagggg aaaggttatt tcgtttttca
     4141 cgatgcttac ggctatttcg aaaaacagtt cggactgaca ccgcttggtc attttaccgt
     4201 taaccctgag attcaacctg gcgcgcagcg tttacatgaa ataagaacac agttggttga
     4261 gcaaaaagca acctgcgttt ttgctgagcc acagttcagg ccagcggtcg ttgagagcgt
     4321 cgcacgaggg acatccgttc gtatgggaac gttggatccc ctggggacga atatcaaact
     4381 gggtaaaaca agctattcag aattcctgag tcaattagcc aaccagtatg cgagctgcct
     4441 gaaaggagat taatgaggaa gtgattacgt gcaacagata gcccgctctg tcgccctggc
     4501 gtttaataat ttaccgcgac cacaccgcgt tatgttgggg tcgctcaccg ttcttactct
     4561 ggccgtcgct gtctggcggc cctatgttta tcaccgcgat gccacgccaa ttgtcaaaac
     4621 cattgagctg gaacagaacg aaattcgttc gctcttacct gaagccagtg agccgattga
     4681 tcaagctgca caagaagatg aagccattcc ccaggacgaa ctggatgaca aaatcgccgg
     4741 tgaagcgggc gtgcatgaat atgttgtttc cactggcgat acgctaagca gcattctcaa
     4801 tcagtatggt attgatatgg gtgatatcac ccaactggct gcggccgaca aagaattgcg
     4861 taacctgaaa atcggtcaac aactctcctg gacattaacc gcggacggcg aactgcagcg
     4921 cctcacctgg gaagtgtctc gtcgtgaaac ccgaacctat gaccgtactg ccgctaacgg
     4981 ttttaaaatg accagcgaaa tgcagcaagg agagtgggtt aacaatctgc tgaaaggtac
     5041 cgtcgggggg agctttgttg ccagcgccag aaacgccggt ttaaccagcg ccgaagtgag
     5101 cgcagtgatt aaagccatgc agtggcaaat ggatttccgc aaactgaaaa aaggcgatga
     5161 atttgcggtg ttaatgtctc gagaaatgct tgatggtaaa cgtgagcaaa gccagctgct
     5221 gggcgtacgt ttgcgttcag aaggtaaaga ttattacgct atccgcgctg aagatggcaa
     5281 attctacgat cgtaacggta ctggtctggc gaaaggattc ttgcgattcc cgacggcgaa
     5341 acagttccgt atctcgtcta actttaaccc gcgtcgtact aatccggtga ccggtcgcgt
     5401 tgcagcacac agaggtgttg atttcgccat gccgcagggt acgccagtgc tttcagtggg
     5461 tgacggtgaa gtggtggttg ccaaacgcag tggcgcagca ggttattatg tggctattcg
     5521 tcatggtcgc agctacacca cgcgttatat gcacttgcgc aagattctgg tgaaaccggg
     5581 acagaaggtg aaacgtggcg accgtatcgc gctttccggt aataccggac gttcaaccgg
     5641 gccgcatctg cactatgaag tatggataaa ccagcaggcc gtaaacccgc tgacggcaaa
     5701 actgccgcgt accgaagggc tgaccggctc cgatcgtcgc gaattcctgg cgcaggccaa
     5761 agagattgtg ccgcagctac ggtttgatta attaacatcc attcgcagcc ggtacgcagt
     5821 cagtatcggc tttttttatt tggtgcgggg caagttgcgc cgctacacta tcaccagatt
     5881 gatttttgcc ttatccgaaa ctggaaaagc atggaaacga aaaaaaataa tagcgaatac
     5941 attcctgagt ttgataaatc ctttcgccac ccgcgctact ggggagcatg gctgggtgta
     6001 gcagcgatgg cgggtatcgc tttaacgccg ccaaagttcc gtgatcccat tctggcacgg
     6061 ctgggacgtt ttgccggacg actgggaaaa agctcacgcc gtcgtgcgtt aatcaatctg
     6121 tcgctctgct ttccagaacg tagtgaagct gaacgcgaag cgattgttga tgagatgttt
     6181 gccaccgcgc cgcaagcgat ggcaatgatg gctgagttgg caatacgcgg accggagaaa
     6241 attcagccgc gcgttgactg gcaagggctg gagatcatcg aagagatgag gcgtaataac
     6301 gagaaagtta tctttctggt gccgcacggt tgggccgtcg atattcctgc catgctgatg
     6361 gcctcgcaag ggcagaaaat ggcagcgatg ttccataatc agggcaaccc ggtttttgat
     6421 tatgtctgga acacggtgcg tcgtcgcttt ggcggtcgtc tgcatgcgag aaatgacggt
     6481 attaaaccat tcatccagtc ggtacgtcag gggtactggg gatattattt acccgatcag
     6541 gatcatggcc cagagcacag cgaatttgtg gatttctttg ccacctataa agcgacgttg
     6601 cccgcgattg gtcgtttgat gaaagtgtgc cgtgcgcgcg ttgttccgct gtttccgatt
     6661 tatgatggca agacgcaccg tctgacgatt caggtgcgcc caccgatgga tgatctgtta
     6721 gaggcggatg atcatacgat tgcgcggcgg atgaatgaag aagtcgagat ttttgttggt
     6781 ccgcgaccag aacaatacac ctggatatta aaattgctga aaactcgcaa accgggcgaa
     6841 atccaaccgt ataagcgtaa agatctttat cctatcaaat aaaaaaagcc tctcaaatta
     6901 ttgagaggct gtactcgata aaaaatctca atgagtgttc ccactatttt gagtttttag
     6961 gttagtacgc tattgagtta atcgtagcgt gataatttca aaaggtttga actcaagatt
     7021 gcaatattga ttgcaataaa aactgtcatc taacttattt tcttgaagat cgacatattg
     7081 aacgtagtga taattacata ctcacgaaag catggctgaa atttctaatc catgactctc
     7141 atataatctt acgattatac cgtcgccgtc ttcagctaat tttattgttt cgatgataac
     7201 gctgttagta tcgatagcaa aaaatgatag tgaagggggt aattcacccg ggtgcggttt
     7261 gacagggacg acctgtaagg gatgattcat tatataggct tgattaataa caccacccac
     7321 acggtaatct ccatgatgag gaaataagga gaatatgaat ttttgtttac caatatctgc
     7381 aaaattatta atcaggaaat tatcataatt gtctgtctta acaacttccc caggtttatg
     7441 ctgcgaacgc agcaaagtta attcaagcgt ctggtctttg acacgatagc catatttgtt
     7501 ttttgctatt aaagctatgc cagtttctcg atcagaaagg tcgacccagt gatgtgctgg
     7561 tatttcatct ttggcaaaag cgaacgaatc atcgctatgc gttggtctgt caattacacc
     7621 gaactgaatt tgacattgtg caagagatgt ttgtactgac accgggaatt ttactttgag
     7681 agaaacatta ggttcgtgcc aatcaataaa cgtttggaca tcaacgcgtg gactatcctg
     7741 tgtcagggta aaggctaaac gaagataaga ggaattgtag tgataaatca gcgtcatcgt
     7801 agttttaggc ccgctgatca cagttgtcat tttttcagcg agtaaaacat atgattcccc
     7861 atcacgataa tttgaagcaa aatcccaggc cgcgaaaaaa cctgcatctt cgtggtatgc
     7921 acggatctca ctgtgcatat tttcagtaat gtattcttta ccatatcttt tatcatatag
     7981 cgaaattaaa tcaccgcttt ctgaaaaaag aagtttaatg taattatttt caatactttg
     8041 agattctgcc ttcagaccat ttacgatctt attctttgga acaatcacag cataaccata
     8101 agagtttact cgtgctttga gccagttgtt gtttatattg atccattcgt tacgagcaaa
     8161 acaggtggta ttaaacaaaa tgtgcggatc tttataagaa gaagtgt
//
LOCUS       DAZPDU010000184         8182 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZPDU010000184 DAZPDU010000000
VERSION     DAZPDU010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8182
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.186"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..2847
                     /gene="yfaL"
                     /locus_tag="ACHURS_003751"
     CDS             <1..2847
                     /gene="yfaL"
                     /locus_tag="ACHURS_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFR1239377.1"
                     /translation="ADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFT
                     GEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQNQAELNVGSTQQT
                     FVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSYVLSGAQSMALTG
                     DIVVDDGAVLSLEGDAADLAALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSG
                     SSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASG
                     TLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHGRDIEMRADGEVA
                     VDAGVDTQWGALMADSSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDV
                     ADIFPYASSLWVGDGAAFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNA
                     SLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAG
                     DTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTG
                     IKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPNP
                     NPTPDPDPTPDPDPTPDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDG
                     QTLNLRVIGGRYHYTAAGQLAQQEDTSTVQLSGGLFSGRWGTDGEWMLGAVGSYSDNQ
                     GDSRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDNWLQYAWFSNDVSEHEDG
                     TDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDD
                     IQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKRRGEIKVGVTG
                     NISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(2975..3697)
                     /gene="ubiG"
                     /locus_tag="ACHURS_003752"
     CDS             complement(2975..3697)
                     /gene="ubiG"
                     /locus_tag="ACHURS_003752"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFR1239378.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            3844..6471
                     /gene="gyrA"
                     /locus_tag="ACHURS_003753"
     CDS             3844..6471
                     /gene="gyrA"
                     /locus_tag="ACHURS_003753"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFR1239379.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            6679..>8182
                     /locus_tag="ACHURS_003754"
     CDS             6679..>8182
                     /locus_tag="ACHURS_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFR1239380.1"
                     /translation="MVLLSAGVWALQYAGSGPEKTLSPLVVHNNLQIDLNEPDLFLDS
                     DSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIEGSIRRIVYEHDLTLKDKLFS
                     SLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPLLFAATSDSQLSKTEISSIKI
                     NSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFKDDQQDTEATAIAGDLLSGKK
                     RWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMPSFAGVRFEMGNDGWHSFVAL
                     NDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAEEMLSHISQENDKLNGALDGA
                     AGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNIGAHESKAPEGVLPISQTQQG
                     EAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLAMQNKTLLFSLDDTLVNNALQ
                     TLNKNRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLTSLPKNLEPVFYNA"
BASE COUNT         1979 a         2031 c         2321 g         1851 t
ORIGIN      
        1 gccgacggaa aaacgctggt tattggcaat acagagaatg acggagctgt tgactctatt
       61 gctggtaccg ggttgatcac caaaacaggt tccggcgatc tggtacttaa tgcagataac
      121 aatgacttta ctggtgagat gcagattgaa aacggtgaag ttaccctggg ccgcagcaac
      181 tccctgatga atgtcggcga tacgcattgc caggacgatc cgcaagactg ctacggtctg
      241 acgataggga gtattgataa gtaccagaat caggcagagc taaacgttgg ctccacccaa
      301 caaacctttg tgcactcatt gacgggcttt cagaatggca ctttaaatat cgatgctggt
      361 ggcaatgtta ctgttaatca aggcagtttt gctggcacca tcgaaggtgc tggtcagctc
      421 accattgcgc aaaacggcag ctacgtgctg tcgggggcgc agtcgatggc gctaaccggc
      481 gatatagtcg ttgatgatgg tgcggtgctt tcgctggaag gcgacgcggc agatctcgcc
      541 gctctccagg acgatccgca gtcgatcgtg ttaaacggcg gtgtgctcga tctctctgat
      601 ttctccacct ggcagagcgg cacatcatac aatgatggcc ttgaagtcag tggcagcagc
      661 ggaacggtta tcggcagtca ggatgtggta gatcttgcag gtggcgacaa tttgcatatc
      721 ggcggcgacg ggaaagatgg cgtctacgtg gtggtcgatg cgagcgacgg gcaggtaagt
      781 ctggcaaaca ataatagtta tttgggcaca acacaaatcg cctccggtac gctgatggtg
      841 agcgacaact cgcagcttgg agatacccac tataaccgcc aggttatctt taccgataag
      901 caacaagaaa gcgtgatgga gattactgcc aatgtcgata ctcgctctac gacgactgag
      961 catgggcgtg atattgaaat gcgcgccgac ggtgaagtgg cagttgatgc gggggtagac
     1021 acgcagtggg gcgcactgat ggctgacagc agcgggcagc atcaggatga gggtagcaca
     1081 tttactaaaa cgggggcggg tacgctggag ctgaccgcca gcggtacaac gcagtcggca
     1141 gtacgtgtcg aagaaggcac cctgaaaggc gatgttgcgg atatctttcc ttatgcttcg
     1201 tcactgtggg tcggtgatgg ggcggcgttc gttactggcg cggatcagga tattcagtct
     1261 atcgatgcta cttccagcgg cactatcgat atcagcgatg gtacggtttt gcgtctgacc
     1321 gggcaggata cttccgtcgc ccttaatgcc tcattgttta acggcgatgg gacgctggtg
     1381 aatgctaccg atggcgtgac gttgacaggt gagcttaata ccaaccttga aactgacagc
     1441 ctgacctatc ttgccgacgt gacggttaat ggtgatctga ccaatacgtc cggtgcggtt
     1501 agcctgcaaa atggcgtcgc tggcgatacg ctgacggtaa acggtgatta taccggcggc
     1561 ggtacgctac tgctcgatag cgaattaaac ggcgatgact cggtaagcga tcaattggtg
     1621 atgaacggta atactgctgg caataccacg gtggtggtta actctattac agggattggt
     1681 gagccgacat cgacaggcat taaagtggtt gatttcgcag ctgatcccac gcagtttcaa
     1741 aacaatgcgc agttcagtct ggcaggcagc ggctacgtca atatgggagc gtatgactac
     1801 acgctggtgg aagataacaa cgactggtat ctgcgatcgc aagaagtaac gccgccatcg
     1861 ccacctaatc ccaacccgac tcccgatcct gatcccacac cggatcctga cccaaccccc
     1921 gacccggccc ctacgcctgc ttaccagccg gtgctgaatg ccaaagttgg cggttatctc
     1981 aataacctgc gggcggcaaa tcaggcgttt atgatggagc gacgcgatca cgctggtggc
     2041 gatggtcaga cgctgaattt acgtgttatc ggcggacgtt atcattacac ggcggcaggg
     2101 caactggctc agcaggaaga cacttctacg gtgcagctta gcggcggctt gtttagcggg
     2161 cgctggggca cggatggcga gtggatgctt ggggcggttg gtagctacag tgataatcag
     2221 ggcgacagcc gctcgaatat gaccggaact cgcgccgata accagaacca cggttatgcc
     2281 gttgggctga cctcaagctg gtatcagcac ggtaatcaga agcagggggc ctggctggat
     2341 aactggttgc agtacgcgtg gtttagcaat gatgtttctg aacatgaaga tggcacagat
     2401 cattaccatt cgtcggggat tatcgcctcg ctggaagccg ggtatcagtg gttaccgggg
     2461 cgtggtgtgg tgattgaacc gcaggcgcag gtgatttatc agggcgtgca gcaggatgat
     2521 tttaccgccg ctaaccatgc gcgcgtgtca caatcgcagg gggatgatat tcagacgcgg
     2581 ctgggtttac acagcgaatg gcgtaccgcg gttggtgtca caccaacatt agatctgaat
     2641 tattatcacg atccccatgc gacggaaatt gaagaagatg gcagcactat cagtgacgat
     2701 gcggtgaagc gacggggtga aataaaagtg ggagtcacgg gcaatatcag tcagcgagtg
     2761 tccctgcgcg gcagcgtggc gtggcagaaa gggagtgatg attttgccca gacggcaggg
     2821 tttttgtcga tgacggtgaa atggtaatcg ctgtctgacc atcacaattg ccggatgcgg
     2881 cgtaaacgcc ttatctggcc tacggagagc atttgtaggc ctgataagac gctgcaagcg
     2941 tcgcatcagg cattggcgcg gccaaacatc aacctcactt attctgcgtg tgcagcatat
     3001 agttcacatc cacgccgggg ccgagcttaa aggaattagt gatcgggttg taatgtagcc
     3061 cggttatatg tcgctctttt aaactggtct gatccaccca gccaagcagc tctgccggtt
     3121 taataaactt cttcacatca tgcgtacctt tgggcaccat gcgcaaaata tattccgcgc
     3181 caaccaccgc catcagccat gacttgccgt tgcggttaag cgtcgagaaa aagacatcgc
     3241 cgcctggttt caccagttgc gcacaggctc tgaccactga ctgcggatcg ggtacgtgct
     3301 ccagcatctc catgcaggtc accacatcat actgcccggc atgttttgcc gcgtgctctt
     3361 ccacggtttc ctgcacgtaa tccacctgaa tgccgctttc cagcgcgtgc agctttgcca
     3421 cctgcaatgg ctcaaagccc atatccagac cggtcaccgt cgcgccttcg cgcgccatac
     3481 tctcggccag aatgccgccg ccacaaccga catcgagcac ctttttgcca aataaaccgc
     3541 cagcacgctc ggcaatatag cccagacgca gcgggttaat gcggtgcaac ggtttgaact
     3601 caccttcaag atcccaccag cgggaggcga cggcttcaaa tttagcgatc tcttcgtggt
     3661 ctacgttatg gttttccggc gatttttcgg cattcattgg cacttctact ccgtaattgg
     3721 caagacaaac gagtatatca ggcattggat gtgaataaag cgtataggtt tacctcaaac
     3781 tgcgcggctg tgttataatt tgcgaccttt gaatccggga tacagtagag ggatagcggt
     3841 tagatgagcg accttgcgag agaaattaca ccggtcaaca ttgaggaaga gctgaagagc
     3901 tcctatctgg attatgcgat gtcggtcatt gttggccgtg cgctgccgga tgtccgagat
     3961 ggcctgaagc cggtacaccg tcgcgtactt tacgccatga acgtactagg caatgactgg
     4021 aacaaagcct ataaaaaatc tgcccgtgtc gttggtgacg taatcggtaa ataccatccc
     4081 catggtgact cggcggttta tgacacgatc gtccgtatgg cgcagccatt ctcgctgcgt
     4141 tacatgctgg tagacggtca gggtaacttc ggttccatcg acggcgactc tgcggcggca
     4201 atgcgttata cggaaatccg tctggcgaaa attgcccatg aactgatggc cgatctcgaa
     4261 aaagagacgg tcgatttcgt tgataactat gacggcacgg aaaaaattcc ggacgtcatg
     4321 ccaaccaaaa ttcctaacct gctggtgaac ggttcttccg gtatcgccgt aggtatggca
     4381 accaacatcc cgccgcacaa cctgacggaa gtcatcaacg gttgtctggc gtatatcgat
     4441 gatgaagaca tcagcattga agggctgatg gaacacatcc cggggccgga cttcccgacg
     4501 gcggcaatca ttaacggtcg tcgcggtatt gaagaagctt accgtaccgg tcgtggcaag
     4561 gtgtacatcc gcgcccgtgc tgaagtggaa gtcgacgcca aaaccgggcg tgaaaccatt
     4621 atcgtccacg aaattccgta tcaggtaaac aaagcgcgcc tgatcgagaa gattgcggaa
     4681 ctggtaaaag aaaaacgcgt ggaaggcatc agcgcgctgc gtgacgagtc tgacaaagac
     4741 ggtatgcgca tcgtgattga agttaaacgc gatgcggtcg gtgaagttgt gctcaacaac
     4801 ctctactccc agacccagtt gcaggtttct ttcggtatca acatggtggc attgcaccat
     4861 ggtcagccga agatcatgaa cctgaaagat atcatcgcgg cgtttgttcg tcaccgccgt
     4921 gaagtggtga cccgtcgtac tattttcgaa ctgcgtaaag ctcgcgatcg tgctcatatc
     4981 cttgaagcac tagccgtggc gctggcgaac atcgacccga tcatcgaact gatccgtcat
     5041 gcgccgacgc ctgcagaagc gaaaactgcg ctggtggcta atccgtggca gctgggcaac
     5101 gttgccgcga tgctggaacg tgctggcgac gatgctgcgc gtccggaatg gctggagcca
     5161 gagttcggcg tgcgtgatgg tctgtactac ctgaccgaac agcaagctca ggcgattctg
     5221 gatctgcgtt tgcagaaact gaccggcctt gagcacgaaa aactgctcga cgaatacaaa
     5281 gagctgctgg atcagatcgc ggaactgttg cgtattcttg gtagcgccga tcgtctgatg
     5341 gaagtgatcc gtgaagagct ggagctggtt cgtgaacagt tcggtgacaa acgtcgtact
     5401 gaaatcaccg ccaacagcgc agacatcaac ctggaagatc tgatcaccca ggaagatgtg
     5461 gtcgtgacgc tctctcacca gggctatgtt aaatatcagc cgctttctga atacgaagcg
     5521 cagcgtcgtg gcgggaaagg taaatctgcc gcacgtatta aagaagaaga ctttatcgac
     5581 cgactgctgg tggcaaacac gcacgaccat attctgtgct tctccagccg tggtcgcgtc
     5641 tattcgatga aagtttacca gttgccggag gccactcgtg gcgcgcgcgg tcgtccgatc
     5701 gtcaacctgc tgccgctgga gcaggacgaa cgtatcaccg cgatcctgcc agtgaccgag
     5761 tttgaagaag gcgtgaaagt cttcatggcg accgctaacg gtactgtgaa gaaaaccgtc
     5821 ctcaccgaat tcaaccgtct gcgtaccgcc ggtaaagtgg cgatcaaact ggttgaaggc
     5881 gatgagctga tcggcgttga cctgaccagc ggcgaagacg aagtaatgct gttctccgcc
     5941 gaaggtaaag tggtgcgctt taaagagtct tctgtccgtg cgatgggctg caacaccacc
     6001 ggtgttcgcg gtattcgctt aggtgaaggc gataaagtcg tctctctgat cgtgcctcgt
     6061 ggcgatggcg caatcctcac cgcaacgcaa aacggttacg gtaaacgtac cgcagtggcg
     6121 gaatacccaa ccaagtcgcg tgcgacgaaa ggggttatct ccatcaaggt taccgaacgt
     6181 aacggtttag tggttggcgc ggtacaggta gatgactgcg accagatcat gatgatcacc
     6241 gatgccggta cgctggtacg tactcgcgtt tcagaaatca gcatcgtggg ccgtaacacc
     6301 cagggcgtga tcctcatccg tactgcggaa gatgaaaacg tagtgggtct gcaacgtgtt
     6361 gctgaaccgg ttgacgagga agatctcgat accatcgacg gcagtgccgc ggaaggggac
     6421 gatgaaatcg ctccggaagt ggacgttgac gacgagccag aagaagaata attttacttc
     6481 ttcatgccaa aagggagcta tctcccttgt ttgaattgaa aagtccaggc tgcaaagtct
     6541 gggcttttgt cgtattaggg cacggtaaag tttggctgtg cccgtaaaaa atggctggct
     6601 atacacaagg aatgtggcaa tgagtggtga aaaaaagcga aaggctggcg gttctatggt
     6661 cttgtaggtt ttggcgcaat agtactgctt tccgctggtg tctgggcgtt gcaatatgct
     6721 ggcagtgggc cagaaaaaac gttgtcgccg ctggtggtgc acaacaatct gcaaatcgat
     6781 ctcaatgagc cggacctctt tctcgacagc gactctctga gccagcttcc caaagatctc
     6841 ctcaccattc cgtttctcca cgatgttctg agtgaagatt tcgttttcta ttatcagaat
     6901 catgccgatc gtctgggcat tgaaggcagc attcgtcgca ttgtctatga acacgatctc
     6961 acgctgaaag ataaactctt ttcgtcactc ttagatcagc ccgcgcaggc tgcgctatgg
     7021 cacgataaac aaggccatct ttcacattac atggtgctga tccagcgcag tggtttaagc
     7081 aaactgctgg agccattgtt gtttgccgct accagcgaca gccagttaag caaaacggaa
     7141 atcagtagca tcaagataaa tagtgaaact attcctgttt atcagttgcg ctataacggc
     7201 aataacgccc tgatgttcgc gacttatcag gacaagatgc tggtgttttc cagcacggat
     7261 atgttgttta aagatgatca gcaggatacc gaagccacgg cgatcgcagg tgatttgttg
     7321 agcggcaaaa aacgctggca agcaagcttt ggcctggaag agcgtgctgc tgaaaaaacg
     7381 ccagtacgcc agcgcatcgt ggtcagcgcc aggttgctgg gttttggcta ccagcggtta
     7441 atgccttctt ttgctggcgt acgcttcgaa atgggtaacg acggctggca cagttttgtg
     7501 gcgttaaatg atgaatccgc cagcgtagat accagtttcg attttacgcc ggtctggaac
     7561 agtatgcctg ccggggccag cttctgtgtg gtggtgccgt attcacacgg tattgccgaa
     7621 gagatgcttt cgcacatcag ccaggaaaac gacaagctga atggggcgtt agacggtgcc
     7681 gcggggctgt gctggtatga agactcaaaa ttgcaaaccc cgctgtttgt cggtcagttt
     7741 gatggcactg ccgaacaggc gcaattgcca gggaaactgt ttacgcaaaa tattggtgcg
     7801 cacgaaagca aagcgccaga aggtgttttg ccgataagcc agactcagca gggcgaagcg
     7861 caaatctggc gtcgcgaagt gagttcccga tacggtcagt atccgaaagc gcaggcggcg
     7921 caacccgatc aattaatgtc ggattatttt ttccgcgtgt cgctggcgat gcaaaacaaa
     7981 acgctgcttt tctccctcga tgacacgctg gttaataacg ccctgcaaac actgaataaa
     8041 aaccgcccgg caatggtgga tgtaataccc actgatggca tcgttccgct ctatatcaat
     8101 ccacaaggcg tggcgaaact gctgcgtaac gaaacgttga ccagtctgcc gaagaatctc
     8161 gaaccggttt tttataacgc cg
//
LOCUS       DAZPDU010000185         8157 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZPDU010000185 DAZPDU010000000
VERSION     DAZPDU010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8157
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.187"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..254
                     /locus_tag="ACHURS_003755"
     CDS             <1..254
                     /locus_tag="ACHURS_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFR1239381.1"
                     /translation="RGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRY
                     FLALEEGVNLLPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(303..1559)
                     /gene="nupG"
                     /locus_tag="ACHURS_003756"
     CDS             complement(303..1559)
                     /gene="nupG"
                     /locus_tag="ACHURS_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFR1239382.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(1761..2840)
                     /gene="mltC"
                     /locus_tag="ACHURS_003757"
     CDS             complement(1761..2840)
                     /gene="mltC"
                     /locus_tag="ACHURS_003757"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFR1239383.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(2905..3180)
                     /locus_tag="ACHURS_003758"
     CDS             complement(2905..3180)
                     /locus_tag="ACHURS_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFR1239384.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(3208..4260)
                     /gene="mutY"
                     /locus_tag="ACHURS_003759"
     CDS             complement(3208..4260)
                     /gene="mutY"
                     /locus_tag="ACHURS_003759"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFR1239385.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            4421..5140
                     /gene="trmB"
                     /locus_tag="ACHURS_003760"
     CDS             4421..5140
                     /gene="trmB"
                     /locus_tag="ACHURS_003760"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFR1239386.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            5140..5466
                     /locus_tag="ACHURS_003761"
     CDS             5140..5466
                     /locus_tag="ACHURS_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFR1239387.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            5650..6369
                     /locus_tag="ACHURS_003762"
     CDS             5650..6369
                     /locus_tag="ACHURS_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFR1239388.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            6545..7591
                     /gene="ansB"
                     /locus_tag="ACHURS_003763"
     CDS             6545..7591
                     /gene="ansB"
                     /locus_tag="ACHURS_003763"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFR1239389.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            7708..>8157
                     /locus_tag="ACHURS_003764"
     CDS             7708..>8157
                     /locus_tag="ACHURS_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFR1239390.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNY"
BASE COUNT         2087 a         1988 c         2161 g         1921 t
ORIGIN      
        1 ttcgcggtga agtggagttg gtgcggattc gtgatgccga agggcgaatt gcggcagaag
       61 gggcgttgcc ttatccacct ggcgtgcttt gcgtggttcc cggggaagtc tggggtgggg
      121 cggttcaacg ttatttcctt gcgctggaag aaggcgtgaa tttgttgccg ggattttcgc
      181 cggagttgca aggtgtctat agcgaaaccg atgcggatgg catgaaacga ttgtacggtt
      241 atgtgttgaa gtaagaataa aaaaacgggt caccttctgg cgacccgttt ttctttgcgt
      301 aattagtggc taaccgtctg tgtgcctgtc gggacacgaa cgtgtttata tttgaacatc
      361 gccatgaacg cgaaggccag aaccacggaa taaccagcga aaatcaacca tacggtctgc
      421 cagtcggtaa tgccgttttg ggtgtacatc tcaacaactt taccgctcac gatgccgccg
      481 aggatacagc cgaagccgtt agtcatcatc aggaacatcc cttgtgcact ggcgcgaatt
      541 gccgggctaa cttctttttc gacaaacacc gaaccagaga tgttgaagaa gtcgaatgcg
      601 caaccgtaaa cgatcatcga cagtaccagc agtacagtac cgaacggagt cgggtcgccg
      661 taagcaaaca gcgcaaaacg caggatccac gccacaatac tgatcatcat tacgttctta
      721 ataccgtagc ggcttaagaa gaacgggatg gtcagaatga acagggtttc agagatctgc
      781 gaaatcgaca tgatgattga cgcatgctgc acgatgaagc tgctggcaaa catcggatct
      841 ttgtcgaagc tgtgcaggaa ggtattaccg aacatgttgg taatctgcag ttccgcgccc
      901 agcagcattg agaagatgaa gaagattgcc atacgcttgt ttttaaacag cgcgaatgca
      961 tcgaggccca gcagggttgt ccagctctga ttcgcttgct gtttagcaac cggaatatgc
     1021 ggcagagtca gggtaaacag aaccagaatg gcggaaagtg ctgcgccaat atacagctgc
     1081 atgtggctta attcgaagcc agacaggctc accacccaca ttgccatgat aaagccgatg
     1141 gtgccccaga tacggattgg cgggaagtca gtaacgatat ccatcccggc attttgcagg
     1201 cgatagtaag agatggtgtt gattaaccca agcgttggca tataagcaaa cgagttaatc
     1261 aatatcacaa ggaacatcgc ttccggcgtc gtgacctgtg ccgccatgaa cagcgtgata
     1321 gcgccaatgg tgtggcaaat ggcatatacc catttcgcac ttaaccattt gtcggccaca
     1381 atccccagca gcgcaggcat aaataccgcg gcgataccca gtgagctata aactgcgcca
     1441 atagaagcac cgtcaaactt cagggtaaca aacatatagg agccgagggt cgtcagccaa
     1501 cttccccaca gacagaactg cagaaaagag aggattttca gctgcagctt aagattcatg
     1561 ttaatttcct cacattgtga tgcggatgag tgtttgaaaa agcacatttg accgtgcgag
     1621 atgatgggat tctatcaact tcgcggactg gttttttgtt acctgtggca aataattgca
     1681 aacattccca atttgcaaaa cgaaattgtg atgtggataa catttttgcc cctgagcatc
     1741 gtcaggggcg gttaatggaa ttatcggcgg cggtaggatt tttgcgcggt attcacttta
     1801 taaagataac ggcgagattc cgcagaggga tggcgggtcg tcagcgtctg ataaacatcg
     1861 cccggcgtca tggtgttaat aatattggca gcctgaatct tatcattcga aaagactcgc
     1921 agcacgctgc ctgcgccgcc gttataggcg gtgatgacgg cataacgccg cgatgttggg
     1981 ttatcaattc cgccgagata aacattgttc agcatcgcca gatacgcggt gccggtatca
     2041 atattgctgg caggatcaaa caagaaactg cggctcggcg tgccggattt cccctgcgag
     2101 cggaacacat ctttcccggc agtatgttgt accacctgca ttaatcccag cgcatcggaa
     2161 cggctgaccg catacgggtt aaaggaagat tcggtctgca taattgccag aatcagcgac
     2221 tcatcaacgc catatttccg tgacgcctgg cggaccatgc cgagatattt gtgcgcacgt
     2281 ttatcaaggt ggttcggtac catgttaatg gtgacgctgt agatgatacg cagcccgtta
     2341 ctgcggcttt gcagacggtt tttcagcaga taatccgcga agttgcttgc gcgaccttcc
     2401 cagcgaatcg gctgcccggt gttgtccacc acctgaccgt aaaggaaagg ttctttcgaa
     2461 atcgtaatat catcaacgtc ggaatagaga tcgaccgaac tcggatcgtc acccatcagc
     2521 aacgttttga taattgcccg gcgcaaatgc gcggcaggtt ctgtcccggc gatagtttcg
     2581 atagtaatcg taccgtcatc gaagttgatg tggctgcggg tctgatattg atcggtgtat
     2641 ttcacgtagt ccttaggacc ggcgatcacc acctctttga atccccagat gttctcaata
     2701 ttgtgggcaa attgccccat cagaatatca aaaccgttgg tatctttgac ccaggcttcg
     2761 ttataggtat cgcctttttt ggtcgtcgaa caggagatga gcaacggcgc aatcaaagcc
     2821 agcgcgagat attttttcat cattccgggt gcgtgttgtg tttgttttta tgcaagttgc
     2881 cggaggcgtg ctccggcact gtttttattt tttatcttcc ggcgtatagc cctcgatatg
     2941 cacctcttta ccctcgaaca ggaagttgac catctcctgc tcaagcagct tgcggtgctc
     3001 ggcattcatc atgttgagtt tcttttcatt aatcagcatg gtttgcttgt gctgccactg
     3061 cgcccaggct tctttggaga tctcgttata gatgcgtttt cccagctcgc cggggtacag
     3121 ctgaaaatcc tgaccttctg cttcacgttg caggaaagta caaaaaatcg ttctgctcat
     3181 aaatcatcct ctttatcgac tcacgcgcta aaccggcgcg ccagtgcgta actgctgtaa
     3241 caaacgctcc acgggagccg ccaggccaac tgacggcggt tgcgctaagt tataccagag
     3301 cgcattgcct tcatccatgc agccggtgaa tgacgacacg ggaagccaca taggcacaat
     3361 atctaagtgg aaatggctga aggtatgccg aaacgcggtc agttgcgtca ggttatcggc
     3421 agcaatctgc cgttgcgcca gccactgccg caaactttct tcgtcggcaa actgcgggaa
     3481 acagtataaa ccgccccaca atccgctcgg cggacgctgc gccagcaata cttcatcttc
     3541 gtgctgtaac agcaaaaagt agccggtgcg ctccggcagc gtctgtttcg gttttttgcc
     3601 cggataaagc gaccagctat tattggcggc ggcaatacat ccgttttgta gcggacagag
     3661 cgaacatttc ggcttcgagc gcgtacaaat catcgcgccc aaatccatca tcgcctgatt
     3721 aaaccgttcc acgccgaccg cgggcgtcac ctgctcgctc aaactccata atttattctc
     3781 gacctctttt ttcccaggcc agccgcttac agcatagcag cgcgccagca cgcgtttgac
     3841 gttaccgtcg agaatcggaa agtgcttacc cagagaaagc gagagaatcg cgcctgcggt
     3901 ggaacgcccg acgcccggta acgccgcgac ttcttcaaag gtttccggga atttaccgcc
     3961 gtgtaaggtc gccacttgtt gtgccgcttt atgcagattg cgcgcgcggg cgtaatagcc
     4021 aagcccggtc cacaagtgga gaacttcgtc gagcggcgca ttggcgagat cggtcaccgt
     4081 cgggaagcgc gccataaagc gttcaaaata ggggataacg gtcgcaacct gagtttgttg
     4141 caacatcact tctgagagcc atactttgta gggcgtcttg tcaatttgcc agggcagcgt
     4201 ttttcgcccg tatttatcgt accagtccag aacctgggct gaaaattgcg acgcttgcat
     4261 ggtcaccgaa ttcactgttg ttgggggcaa gattgcagca cagcgacggc agggtgtaaa
     4321 ccggaacttt ccgcagccac ggccttatca tgcttgcatc cggcaactaa ctttggataa
     4381 tgcccgtttt cagaacactt tcacaagcga ctaaatcttt atgaaaaacg acgtcatttc
     4441 accggaattt gatgaaaacg gccgcccact gcgccgtatc cgtagttttg tgcgccgcca
     4501 ggggcgactg accaaaggcc aggaacatgc gctggaaaac tactggccgg tgatgggcgt
     4561 tgagttcagc gaagatatgc tggatttccc cgcgcttttt ggccgtgaag cgccggtgac
     4621 gcttgagatt ggttttggca tgggggcgtc gctggtggca atggctaaag atcgccctga
     4681 gcaggacttc ctcggcattg aagtgcattc accgggcgtt ggtgcgtgcc tggcttctgc
     4741 gcatgaagaa ggtttaagca acctgcgcgt gatgtgtcac gatgcggttg aagtgctgca
     4801 taaaatgatt cctgacaatt cattgcgcat ggtgcagctc tttttccctg acccgtggca
     4861 caaagcgcgc cataataaac gccgtatcgt acaggtgccg tttgccgaac tggtaaaaag
     4921 caaactgcag ctggggggtg tattccatat ggcgaccgac tgggaacctt atgcggaaca
     4981 tatgcttgaa gtgatgtctt ctattgacgg ttataaaaac ctgtcagaga gcaatgatta
     5041 cgtgccgcgt ccggcatcac gtccggtgac gaaatttgaa caacgtggtc atcgtcttgg
     5101 tcacggagta tgggacttaa tgttcgagag ggtgaaataa tggcaaagaa ccgtagccgt
     5161 cgtctgcgta aaaaaatgca catcgacgaa ttccaggaat taggattttc ggtggcatgg
     5221 cgattcccgg aaggtacatc ggaagaacag attgataaaa ccgttgatga ttttattaac
     5281 gaggttatcg aaccgaacaa actggccttt gacggcagcg gttatctggc ctgggaaggt
     5341 ctgatctgca tgcaggaaat cggcaaatgc accgaagaac atcaggcgat tgtgcgtaag
     5401 tggctggaag agcgcaaact ggatgaggta cgcaccagcg aacttttcga cgtttggtgg
     5461 gactaagaaa gcatacgggc gatgacaaat gcaaaactgc ctgatgcgct acgcttatca
     5521 ggcctggaaa gatgcacgat cgagtaggcg ggataaggtg tttacgccgc atccggcatg
     5581 gaaaacgcgt actttgttat caatctgggg ccagcaaatg ctggcctgat tttttcttga
     5641 gggaagacta tgatgcgcaa aatgctgctg gcggcagcac tttcagtgac ggcaatgacc
     5701 gctcacgccg actaccagtg cagcgtcacg ccgcgtgacg atgtgattgt cagcccgcaa
     5761 accgtgcagg tgaagggcga aaacggcaat ctggtgatca cgccagacgg caacgtgatg
     5821 tataacggta agcaatattc cctgaatgcc gcccagcgcg agcaggcgaa ggattatcag
     5881 gctgaactac gcagcacgct gccgtggatt gatgaaggcg cgaaaagccg cgtcgaaaaa
     5941 gcccgtattg cgctggataa aattatcgtt caggagatgg gcgaaagcag caaaatgcgc
     6001 agccgtctga ccaaacttga tgcgcagctg aaagagcaga tgaaccgcat tatcgaaacg
     6061 cgcagcgatg gcctgacgtt tcactataaa gccattgatc aggttcgcgc cgaaggccag
     6121 caattagtga atcaggcaat gggtggaatt ttacaggaca gcattaatga aatgggcgcg
     6181 aaagcggtgc tgaaaagcgg cggtaaccca ttacagaatg tgctgggaag cctggggggg
     6241 ctgcaatcct caatccaaac agagtggaaa aagcaggaaa aagatttcca gcagtttggc
     6301 aaagatgttt gtagccgcgt tgtgactctg gaagatagcc gcaaagccct ggtcgggaat
     6361 ttaaaataat cctctatttt aagacggcat aatacttttt tatgccgttt aattcttcgt
     6421 tttgttacct gcctctaact ttgtagatct ccaaaatata ttcacgttgt aaattgttta
     6481 acgtcaaatt tcccatacag agctaaggga taatgcgtag cgttcacgta actggaggaa
     6541 tgaaatggag tttttcaaaa agacggcact tgccgcactg gttatgggtt ttagtggtgc
     6601 agcattggca ttacccaata tcaccatttt agcaaccggc gggaccattg ccggtggtgg
     6661 tgactccgca accaaatcta actacacagc gggtaaagtt ggcgtagaaa atctggttaa
     6721 tgcggtgccg caactaaaag acattgcgaa cgttaaaggc gagcaggtag tgaatatcgg
     6781 ctcccaggac atgaacgata atgtctggct gacactggcg aaaaaaatta acaccgactg
     6841 cgataaaacc gacggcttcg tcattaccca cggtaccgac acgatggaag aaaccgctta
     6901 cttcctcgac ctgacggtga aatgcgacaa accggtggtg atggtcggcg caatgcgccc
     6961 gtccacgtcc atgagcgcag acggtccatt caacctgtat aacgcggtag tgaccgcagc
     7021 tgataaagcc tccgctaatc gtggcgtgct ggtggtgatg aacgacaccg tactggacgg
     7081 tcgcgatgtc accaaaacca acaccaccga cgtagcgacc ttcaagtctg ttaactacgg
     7141 tcctctggga tacattcaca acggtaagat tgactaccaa cgtaccccgg cacgtaagca
     7201 caccagcgat acgccattcg atgtctctaa gctgaatgag ctgccgaaag tcggcatcgt
     7261 ttataactac gctaacgcat ccgatcttcc ggctaaagca ctggtagatg cgggctatga
     7321 tggcatcgtt agcgctggtg tgggtaatgg taacctgtat aaatccgtgt tcgacaccct
     7381 ggcaaccgcc gcgaaaaacg gcactgcagt agtgcgttct tcccgcgtac cgacgggtgc
     7441 taccactcag gatgctgaag tggatgatgc gaaatacggc ttcgtcgcct ctggcacgct
     7501 gaacccgcaa aaagcgcgcg tcctgctgca gctggctctg acgcaaacca aagatccgca
     7561 gcagatccag cagatcttca atcagtacta atcacctcgc cccggtatcg tgccggggct
     7621 ttttcacttc agacttacgt ccattgccaa ttttcattac cctaatgata atcaccggaa
     7681 taaattattc cgcgtgaggt ttttcgggtg aaaaagcaat ggattgttgg cacggcactg
     7741 cttatgttga tgactggtaa tgcctgggca gatggcgaac cgccaactga aaatatctta
     7801 aaagatcaat tcaaaaagca gtatcacggc attctcaagc ttgatgctat caccttaaaa
     7861 aatcttgatg ctaagggtaa tcaggccacc tggtcagcgg aaggcgatgt ctcttccagt
     7921 gacgatctct atacctgggt cggtcagttg gcagattacg aactgctcga acagacctgg
     7981 acgaaagata aaccggtaaa attctcggcg atgttaacca gtaaaggaac gcccgcgtct
     8041 ggctggtcgg tgaactttta ctcttttcag gcggcagcca gcgatcgtgg gcgggtggtc
     8101 gacgatatca aaacgaataa taaatatctg atcgtgaata gcgaagattt caattat
//
LOCUS       DAZPDU010000186         8121 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZPDU010000186 DAZPDU010000000
VERSION     DAZPDU010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8121
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.188"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            98..358
                     /gene="dinJ"
                     /locus_tag="ACHURS_003765"
     CDS             98..358
                     /gene="dinJ"
                     /locus_tag="ACHURS_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HFR1239391.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGI"
     gene            361..639
                     /gene="yafQ"
                     /locus_tag="ACHURS_003766"
     CDS             361..639
                     /gene="yafQ"
                     /locus_tag="ACHURS_003766"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HFR1239392.1"
                     /translation="MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLP
                     AVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            795..1535
                     /gene="dpaA"
                     /locus_tag="ACHURS_003767"
     CDS             795..1535
                     /gene="dpaA"
                     /locus_tag="ACHURS_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFR1239393.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(1506..2273)
                     /locus_tag="ACHURS_003768"
     CDS             complement(1506..2273)
                     /locus_tag="ACHURS_003768"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFR1239394.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(2479..3057)
                     /gene="lpcA"
                     /locus_tag="ACHURS_003769"
     CDS             complement(2479..3057)
                     /gene="lpcA"
                     /locus_tag="ACHURS_003769"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFR1239395.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            3297..5741
                     /gene="fadE"
                     /locus_tag="ACHURS_003770"
     CDS             3297..5741
                     /gene="fadE"
                     /locus_tag="ACHURS_003770"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFR1239396.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLDGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(5784..6230)
                     /gene="ivy"
                     /locus_tag="ACHURS_003771"
     CDS             complement(5784..6230)
                     /gene="ivy"
                     /locus_tag="ACHURS_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFR1239397.1"
                     /translation="MFKTITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDDTYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            6411..7181
                     /gene="yafV"
                     /locus_tag="ACHURS_003772"
     CDS             6411..7181
                     /gene="yafV"
                     /locus_tag="ACHURS_003772"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFR1239398.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPKMF
                     TSGFAMEAAASSLAQDDVVDWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFRLR"
     gene            complement(7223..>8121)
                     /locus_tag="ACHURS_003773"
     CDS             complement(7223..>8121)
                     /locus_tag="ACHURS_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFR1239399.1"
                     /translation="ISPAKFHECFINWMRDCHSSDDKDVIAIDGKTLRHSYDKSRRKG
                     AIHVISAFSTMHSLVLGQIKTDEKSNEITAIPELLNMLDIKGKIITTDAMGCQKDIAE
                     KIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNPEHDSYAISEKSHGREEIRLHIV
                     CDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMTVRYYISSADLTAEKFATA
                     IRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRR
                     KMRKAAMDRNYLASVLAESGLS"
BASE COUNT         1932 a         2035 c         2104 g         2050 t
ORIGIN      
        1 aaaaacatcc gtttattaaa ttactcatgg attgtcgctg ttgctcattt gagctacaat
       61 tcaagctgaa taaatataca gcacaggaga tcccccaatg gctgctaacg cgtttgttcg
      121 cgcccgaatc gatgaagatc tgaagaatca ggcggcggac gtactggccg ggatggggct
      181 gaccatctct gacctggttc gcataaccct cacaaaggtc gcgcgtgaaa aggcattgcc
      241 gtttgattta cgcgagccta atcaattaac cattcaatca atcaaaaaca gcgaagctgg
      301 cgttgatgtt cataaggcca aagacgctga tgatttattt gataaattag gaatttaaat
      361 atgattcaaa gggatattga atactcggga caattttcaa aggatgtaaa acttgcacaa
      421 aagcgtcata aggatatgaa taaattgaaa tatcttatga cgcttcttat caataatgct
      481 ttaccgcttc cagctgttta taaagaccac ccgctgcaag gttcatggaa aggttatcgc
      541 gatgctcatg tcgaaccgga ctggatcctg atttacaaac ttaccgataa acttttacga
      601 tttgagagaa ctggaactca cgcggcgctc tttgggtaaa gcacgctgcc gatgaaattt
      661 gtaaaccctg cgccgacagg cttgcgtaaa actcttttgg tgataatgtg agccttccaa
      721 atccagacta ttccgatttg ggatctattt tgccgtcctg gcgtgtgtaa ccgttttatc
      781 aaggaataag cagtatgcgt aaaatcgcat taattcttgc gatgttgttg atcccgtgcg
      841 tttcgtttgc cggtttgctg ggcagcagta gctcgaccac gccagtgagc aaagagtata
      901 agcagcagtt gatgggatcc cctgtctaca tccagatctt caaggaagaa cgtacgctcg
      961 atctctacgt caaaatgggc gagcaatatc agctgctcga cagttataaa atctgtaaat
     1021 attccggcgg cttagggcca aaacagcgtc agggcgattt caaaagcccg gaagggtttt
     1081 atagcgtcca gcgtaatcag ttaaaaccag acagccgtta ctacaaagcg attaatattg
     1141 gtttccccaa tgcctatgac cgtgcgcatg gttatgaagg gaaatacctg atgatccacg
     1201 gcgattgtgt ttccatcggc tgctacgcaa tgaccaatca gggtattgat gagatcttcc
     1261 agttcgttac tggtgcgctg gtgtttggtc agccgagcgt gcaagtgagt atttacccgt
     1321 tccgcatgac cgacgccaat atgaagcgcc ataaatattc caactttaag gacttctggg
     1381 agcaactgaa gccgggttac gactactttg agcaaacccg taagccaccg accgtttctg
     1441 tggtcaatgg tcgttacgtg gtcagcaagc cgttaagcca cgaagttgtg cagccacaac
     1501 tggcatcaaa ctacacgctc cccgaggcaa aataagcgcc attcgcctgg cataatcttt
     1561 tgccaggttt cattgcccgt cagcggctgt gttgcaatca ccgtgaccac atcattcggt
     1621 gtggtctgcg agctgaagtc gatttccaca tcctgatcca gcagcgttgc cacgccaaac
     1681 ggcgcgcggc gggtgatcca gtgtaaatta gtcgagcaat acgccatcac atagcgcccg
     1741 tcagaaagca gcatgttgaa aacgcccttc tgccgcagtt catccgccag tgaggcgata
     1801 tatttaaaca ctgccgccat gttgcccggt gtgcgcgggt aacgctgcgt taatttatgc
     1861 aggagccagc agaaggcttt ttcgctatcg gtttcaccga ccgggcggaa gttgccggtt
     1921 tccagtgatt tgtagcccgt cagttgtccg ttatgggcat aagtccagtt acgcccccat
     1981 aactcacggg taaacgggtg agtattttcc agcgccacct cgccccgatt ggcctggcga
     2041 atatgagcca ccaccgaaca ggattttatc gggtagtcct ggacaagttt ggcgatgggg
     2101 gaattaaagc tgggttgtgg atctttaaat gtgcgacagc ctttaccttc gtaaaaggta
     2161 atgccccagc catctttatg tggcccggtt ccaccaccac gctgcaccag accggtgaaa
     2221 ctaaagcaga tatcggtagg gacgttggcg ctcatcccga gcaattcgca catgggtaac
     2281 ctccacaaca gcgggagcgc atggcccccg gcgaagattg atttaagaat tttcaggccg
     2341 gatgcggcgt aaacgtctta ttcggcctac ggttcgcgta cagatttgta ggcatgataa
     2401 gacgcatcag cgtcgcatca ggcatcagcg cacaagtgcc ggatgcggcg taaacgcctt
     2461 atccggccta cgccagactt acttaaccat ctctttttca attaactgga tcaggatatg
     2521 gatcacttta atgtgaatct cctgaatgcg gtcggcataa ccaaagtgcg gaacgcgaat
     2581 ttcgatatcc gccgtgccag ccattttacc gccgtcttta ccggtcaggg tgatcacttt
     2641 catccccttc tcacgcgccg ctgcgatcgc tttgatcacg tttgcagagt taccggaggt
     2701 ggagatcccc agcagtacat cgccttcgcg acctaccgct tcaacgtagc gggagaaaat
     2761 atcattgaaa ccgaaatcat taccgacgca ggaaatatga ctaacgtcag aaatagcaat
     2821 cgccgggtag cccggacggt tttcacggta gcgaccggtc aactcttcgg caaagtgcat
     2881 agcgtcgcaa tgggaaccgc cgttgccgca ggaaagcact ttgccaccgg ctttaaagct
     2941 gtctgctaac aggaccgccg cgcgctgaat ggcgtgaata ttggcgtcat cttttaaaaa
     3001 gttagccagc gtttccgccg cttcgttcag ttcgttacga ataagatcct ggtacatgag
     3061 gatatccttc agcataaatg taatagacaa aatgcagtgt accggatacc gccaaaagcg
     3121 agaagtacgg gcaggtgcta tgaccaggac tttttgacct gaagtgcgga taaaaacagc
     3181 aacaatgtga gctttgttgt aattatattg taaacacatt gctaaatgtt tttacatcca
     3241 ctacaaccat atcatcacaa gtggtcagac ctcctacaag taaggggctt ttcgttatga
     3301 tgattttgag tattctcgct acggttgtcc tgctcggcgc gttgttctat caccgcgtga
     3361 gcttatttat cagcagtctg attttgctcg cctggacagc cgccctcggc gttgctggtc
     3421 tgtggtcagc gtgggtactg gtgcctctgg ccattatcct cgtgccattt aactttgcgc
     3481 ctatgcgtaa gtcgatgatt tccgcgccgg tatttcgcgg tttccgtaag gtgatgccgc
     3541 cgatgtcgcg cactgagaaa gaagcgattg atgcgggcac cacctggtgg gagggcgact
     3601 tgttccaggg caagccggac tggaaaaagc tgcataacta tccgcagccg cgcctgaccg
     3661 ccgaagagca agcgtttctc gacggcccgg tagaagaagc ctgccggatg gcgaatgatt
     3721 tccagattac tcatgagctg gcggatctgc cgccggagtt gtgggcgtac cttaaagagc
     3781 atcgcttctt cgcgatgatt atcaaaaaag agtacggcgg actggagttc tcagcttatg
     3841 cccagtctcg cgtgctgcaa aaactctccg gcgtgagcgg gatcctggcg attaccgtcg
     3901 gcgtgccgaa ctcattaggc ccgggcgagc tgctgcaaca ttacggcact gacgagcaga
     3961 aagatcacta tctgccgcgt ctggcgcgtg gtcaggagat cccttgcttt gcactgacaa
     4021 gcccggaagc gggttccgat gcgggcgcga ttccggacac cgggattgtc tgcatgggcg
     4081 aatggcaggg ccagcaggtg ctggggatgc gtctgacctg gaacaaacgc tatattacgc
     4141 tggcaccgat tgcgactgtg cttgggctgg cgtttaaact ctccgaccca gaaaagctgc
     4201 tgggtggcgc agaagattta ggtattacct gtgcgctgat cccgaccacc acgccgggcg
     4261 tggaaattgg tcgtcgccac ttcccgctga acgtaccgtt ccagaacgga ccgacgcgcg
     4321 gtaaagatgt cttcgtaccg atcgattaca tcatcggtgg gccgaaaatg gccgggcaag
     4381 gctggcggat gctggtggag tgcctctcgg taggccgcgg catcaccctg ccttccaact
     4441 caaccggcgg cgtgaaatcg gtagcgctgg caacgggcgc gtatgctcac attcgccgtc
     4501 agttcaaaat ctctatcggt aagatggaag ggattgaaga gccgctggcg cgtattgccg
     4561 gtaatgccta cgtgatggat gctgcggcat cgctgattac ctacggcatt atgctcggcg
     4621 aaaaacctgc cgtgctgtcg gctatcgtta agtatcactg tacccaccgc gggcagcagt
     4681 cgattattga tgcgatggat atcactggtg gcaaaggcat tatgctcggg caaagcaact
     4741 tcctggcgcg tgcttaccag ggcgcaccga ttgccatcac cgttgaaggg gctaacattc
     4801 tgacccgcag catgatgatc ttcggacaag gagcgattcg ttgccatccg tacgtgctgg
     4861 aagagatgga agcggcgaag aacaatgacg tcaacgcgtt cgataaactg ttgttcaaac
     4921 atatcggtca cgtcggtagc aacaaagttc gcagcttctg gctgggcctg acgcgcggtt
     4981 taaccagcag cacgccaacc ggcgatgcca ctaaacgcta ctatcagcac ctgaaccgcc
     5041 tgagcgccaa cctcgccctg ctttctgatg tctcgatggc agtgctggac ggcagcctga
     5101 aacgtcgcga gcgcatctcg gcccgtctgg gggatatttt aagccagctc tacctcgcct
     5161 ctgccgtgct gaagcgttat gacgacgaag gccgtaatga agccgacctg ccgctggtgc
     5221 actggggcgt acaagatgcg ctgtatcagg ctgaacaggc gatggatgat ttactgcaaa
     5281 acttcccgaa ccgcgtggtt gccgggctgc tgaatgtggt gatcttcccg accggacgtc
     5341 attatctggc accttctgac aagctggatc ataaagtggc gaagatttta caagtgccga
     5401 acgccacccg ttcccgcatt ggtcgcggtc agtacctgac gccgagcgag cataatccgg
     5461 ttggcttgct ggaagaggcg ctggtggatg tgattgccgc cgacccaatt catcagcgga
     5521 tctgtaaaga gctgggtaaa aacctgccgt ttacccgtct ggatgaactg gcgcacaacg
     5581 cgctggcgaa ggggctgatt gataaagatg aagccgctat tctggtgaaa gctgaagaaa
     5641 gccgtctgcg cagtattaac gttgatgact ttgatccgga agagctggcg acgaagccgg
     5701 taaagttgcc ggagaaagtg cggaaagttg aagccgcgta attgaataac ggagccgaaa
     5761 ggctccgttt ctttatccgc taattattta aaattaaagc catccggatg gttttccagg
     5821 ctgccggtca acgccgcgaa cagcaccgtt ttaccatcaa tcgaaagcgc atcgttcaca
     5881 ttcagccagg tgagtttctc ttgcgacgtt ttctcatcaa tagtcgagaa cagccccgtc
     5941 atctgattag atttctcgga ccacatcaca gcgatacgtt gcgagccaca gtcatgcggt
     6001 ttgcacgcgc tcatcacctg atacgtatca tctcccaacg ttacggtttg tgcgggagta
     6061 taagtaccgc ctttcatcac ccaggcaggc agcttatgcc cttgtaccat ctgattaaat
     6121 gcagctttgg tggtttcgcc ctttgcaagg ctgctaatgg ttaaatcatc ctgcgccatt
     6181 gcactggtgg cgatgaccag agcggcgact gtcgttattg tcttaaacat cattcctccc
     6241 gagcttatcc tgcccatgtt attaaccttc taaagcctgg cgataacacg ataatttgca
     6301 aatcagatat ttccaatatc agcatttgtt atcaatttat tctgcgctaa ggcaactgcc
     6361 tgataaggtg ttaaagttat aaaacccttc gcataaagga gttgtacacc gtgcctggtt
     6421 tgaagattac gcttttgcag caaccactgg tgtggatgga tggccctgcc aacctgcgtc
     6481 attttgatcg tcaactggaa ggtattaccg ggcgcgatgt gatcgttcta ccgaagatgt
     6541 ttaccagcgg ctttgccatg gaagcggcag cttcgtcgct agcacaagat gacgtagtgg
     6601 actggatgac agctaaggcg cagcagtgca atgcgctgat tgcaggcagt gttgcattac
     6661 aaacggagtc tggttcggtt aaccgctttt tgctggttga gccgggcggc acggtacatt
     6721 tttatgataa gcgtcatctg ttccgcatgg cagatgagca tctacattat aaagcgggca
     6781 atgcgcgagt gattgtggaa tggcgcggct ggcgtatttt gccgttggtg tgctacgact
     6841 tacgttttcc ggtgtggtcg cgcaatctca acgattatga cctcgccatc tacgttgcca
     6901 actggcctgc tccgcgctct ctgcactggc aggcattgct gacggcccgc gcgattgaga
     6961 atcaggcgta tgtggcggga tgcaatcgcg tcggcagcga tggcaacggc tgccattatc
     7021 gcggtgacag ccgggtgatt aatccacaag gcgagattat cgctactgcc gacgcgcatc
     7081 aggcaacgcg cattgatgcg gagctgtcga tgatggcatt gcgggaatat agagaaaagt
     7141 ttccggcatg gcaggatgcg gatgagttta ggttgcggtg agtgggggtt gtgtttaagg
     7201 ctgggggaga gtcggggtaa gactacgaaa gcccgctctc cgcaaggact gacgccagat
     7261 agtttctgtc catagctgct tttcgcatct tacgtcttaa ccctgccttg aataccttat
     7321 cattcgtcaa aatattaata gcgatgtgcc gtatccctga aaataattct gctgcatttc
     7381 ctcttcttat tttgcagtcg tcttcattca ttaccacgtc cagacgccag tgcagcttat
     7441 tctccacgtg ccagtggttt cggattgctg tggcgaactt ctctgcggtt aaatcagcag
     7501 aactgatata atatctgacc gtcatttctg gctctttctt ttgttctgct attatggacc
     7561 gaaaggagac tgccacgcat aatttcttca gccctttcca ttcaaacgtg aaatcaataa
     7621 gttcatcagg gacatcgcaa acaatatgaa gacggatttc ttctctgccg tgactctttt
     7681 cactgattgc gtaactgtca tgctctggat tatttaattc tttcagcgga aatttttcct
     7741 caaaggcttt attaagccgc ccctggtttc cttttaccgc gaataaataa tcaccgccct
     7801 gtttttgtat cttctctgca atatctttct ggcatcccat cgcatcagtt gtgatgattt
     7861 ttcctttaat atccagcatg ttaagaagtt caggaatggc tgtaatctca ttggatttct
     7921 catccgtctt aatctgcccg agaaccagac tgtgcattgt tgagaacgca ctaatgacat
     7981 gaatcgctcc cttgcggcga ctcttgtcat aagagtgccg gagcgttttt ccatcaattg
     8041 caatgacgtc tttatcatct gaagaatggc agtcacgcat ccagttaata aagcactcgt
     8101 gaaattttgc aggactgata c
//
LOCUS       DAZPDU010000187         7980 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZPDU010000187 DAZPDU010000000
VERSION     DAZPDU010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7980
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.189"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(152..487)
                     /locus_tag="ACHURS_003774"
     CDS             complement(152..487)
                     /locus_tag="ACHURS_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFR1239400.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(491..835)
                     /locus_tag="ACHURS_003775"
     CDS             complement(491..835)
                     /locus_tag="ACHURS_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFR1239401.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            complement(837..1010)
                     /gene="ymgI"
                     /locus_tag="ACHURS_003776"
     CDS             complement(837..1010)
                     /gene="ymgI"
                     /locus_tag="ACHURS_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFR1239402.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            1111..1296
                     /gene="ymgJ"
                     /locus_tag="ACHURS_003777"
     CDS             1111..1296
                     /gene="ymgJ"
                     /locus_tag="ACHURS_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFR1239403.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TAFKKCLFLMRDIIISS"
     gene            1257..1376
                     /locus_tag="ACHURS_003778"
     CDS             1257..1376
                     /locus_tag="ACHURS_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFR1239404.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(1855..2121)
                     /gene="minE"
                     /locus_tag="ACHURS_003779"
     CDS             complement(1855..2121)
                     /gene="minE"
                     /locus_tag="ACHURS_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFR1239405.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2125..2937)
                     /gene="minD"
                     /locus_tag="ACHURS_003780"
     CDS             complement(2125..2937)
                     /gene="minD"
                     /locus_tag="ACHURS_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFR1239406.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(2961..3656)
                     /gene="minC"
                     /locus_tag="ACHURS_003781"
     CDS             complement(2961..3656)
                     /gene="minC"
                     /locus_tag="ACHURS_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFR1239407.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            4176..4544
                     /locus_tag="ACHURS_003782"
     CDS             4176..4544
                     /locus_tag="ACHURS_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFR1239408.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(4652..5053)
                     /gene="pliG"
                     /locus_tag="ACHURS_003783"
     CDS             complement(4652..5053)
                     /gene="pliG"
                     /locus_tag="ACHURS_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFR1239409.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            5295..5588
                     /locus_tag="ACHURS_003784"
     CDS             5295..5588
                     /locus_tag="ACHURS_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFR1239410.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            5660..6319
                     /locus_tag="ACHURS_003785"
     CDS             5660..6319
                     /locus_tag="ACHURS_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFR1239411.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            6411..6857
                     /locus_tag="ACHURS_003786"
     CDS             6411..6857
                     /locus_tag="ACHURS_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFR1239412.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMICAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(7064..7975)
                     /gene="hlyE"
                     /locus_tag="ACHURS_003787"
     CDS             complement(7064..7975)
                     /gene="hlyE"
                     /locus_tag="ACHURS_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HFR1239413.1"
                     /translation="MTEIVADKTVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETI
                     KELSRFKQEYSQAASVLVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLFD
                     EYNEKKASAQKDILIKVLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFS
                     EKSSYFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSV
                     QSFFTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKE
                     AAKKMINTCNEYQKKHGKKTLFEVPEV"
BASE COUNT         2054 a         1792 c         1837 g         2297 t
ORIGIN      
        1 ccggcggcca gtgtctgggg taatgtgggc gtgcagctgg gtgataatgg ctacaatgac
       61 accgcagtga tggtgggcct gaaatataag ttctgatccc cgttagctaa aataccgcgt
      121 cgaattcatc gacgcggtaa catattaaat attatttttt gccgatagca cgcatggtgt
      181 catcaattgc cgtgatcaac agcatttgcg ggtctttagc gcaaacctga ttcagttttt
      241 ctaccacttt ggcgctcagt tccggagatt gctcaatttt taaatcacgg ctggcaacgc
      301 tggcattacc catcaccgca acaatttctg caacctgtgc gctgtcagtt tttgccattt
      361 cgttggcttc tgcgcaagta atataggttt ctgacggcaa accgttttta atattgtagt
      421 cctgcgccca ggtcattggt gcgaaaacaa acaggcccgc cagtaaagca aattttttca
      481 tcatcattcc ttatttcatt ttacccagaa ttgcaccacc cgtaccgcca atcacggcac
      541 ctttaatcgc cccttcgagg ccattgccgg tcagaacgcc agtgacagca ccaacggcgg
      601 cacccacttt tgcaccttta cgtgcatttt taccgtcgcg gcctttttct gttactgcac
      661 caacaccagc gccaacagct gcacctttca gtacgccatt aacaccattg ccagtaagta
      721 atccaacgcc tgcgcctagc aatgcacctt tcgtggtgcg gttcatatcc gccatcgctg
      781 gcgtggagca gaacaatgct gagataagcc cgaaggcaag tatttttttc ttcaacttag
      841 atgtccggta ttaagtaagt tgcacacaca ataatttcgt cttcaattaa gatctgctta
      901 actaaagaac gctcgctatt attcagataa ttcaaaatga gcgtggctgt gatgatagga
      961 attatgtttt ttacgtgaat gagaataatc ttaaatgagg aataactcat tgattggcaa
     1021 tatttttatt caagaagtat cattgactgt taacgcaatg ttgtaaaggt aagataatct
     1081 gatttatcaa tattattgtg tgatttttat gtgagcagaa gatattcatc agcaacgatt
     1141 acattagtca ttttattttg ccgacggcct cattgtcgaa agataagcgt acgacagtat
     1201 tatctgaaaa gagtgatttt ttatccaact acacttcagc gcactgcgtt taaaaaatgc
     1261 ctctttctta tgcgggatat catcatttca tcatgatgtc tttggtgagc ggtgaacaca
     1321 atacacctgc gctgtctctt caggatgaat cccctggtct ggtgcctgcg ggctgatgtt
     1381 gcagcagagc ttaggtcact tagacgctac tatcatttat ccaatggcat ggaatcgaaa
     1441 tcagtcgata cccgcagtat atatcgtgaa ctggtcgttc tgaccatcaa cgattaaact
     1501 gcgcttcggc gcagttttcg tttacagggg gctaaaaggg aaaattctgg ggcaaaaaaa
     1561 gcccgccagt tacggcggga aacctcatcc tatgggagaa caatgaataa tgaaattgcg
     1621 gggttatcat cccccagtgt atccacacta acaataaggt tatttactca gccaggcata
     1681 aacattatgt tctgtgcgtg ggaacagcct taaggtgtaa agggggaggt ggaaatagca
     1741 atgaggagta tcagcaagaa tactcgccgc tttaccacaa cgtggatgag agggatgaaa
     1801 aactcaaggc agaaatgact ctgccttgaa gataaatgcg tttttacagc gggcttattt
     1861 cagctcttct gcttccggta aggtcacgtt cagctcaaga atagaaatat cgccatcttt
     1921 ttgctcaagc tgtacggtta ccatctcagg atcaatttgt acgtatttac aaatgacctc
     1981 aagaatatct ttacgcaact gcggcagata atgcggttct gcatcgctgc gacggcgttc
     2041 agcaacaata atctgcagcc gttcttttgc aatgttggct gtgtttttct tccgcgagag
     2101 aaagaaatcg agtaatgcca taacttatcc tccgaacaag cgtttgagga agcctttctt
     2161 ctcttcttca atgaagcgga aaggacgttc ttctcccaac agacgttcta cggtatctgc
     2221 gtaggcttta cccgcatcgg cgttaatgtc gagaatgacc ggttcacctt ggttagaggc
     2281 gcgcaatact gattgatcct ctgggatcac gccgacgagt ttgatgcgca ggatctccag
     2341 cacatcttcc atgctcagca tgtcacctct gcttacgcgg cctgggttat agcgcgttaa
     2401 cagcaggtgc tctttaatag gctcttcgcc attttctgcg cggcgtgatt tcgacgccag
     2461 aatgcctaaa atacggtcag agtcgcgtac tgaggagact tccgggttgg tggtaataat
     2521 ggcttcgtct gcaaaataga gtgccattaa cgcaccggtt tcaatccctg ccggggagtc
     2581 acaaacgata aattcaaaat ccatcgcttt cagatcatca agaactttgg cgaccccttc
     2641 acgggtgagg gcatctttat cgcgtgtttg cgatgccgga agaatataga gattttcagt
     2701 acgcttatct ttaattaacg cctgatttag cgttgcatcg ccctgaatga cgttgacgaa
     2761 atcgtaaacg acccggcgtt cgcaacccat aatcaggtcg agattacgca ggccgatatc
     2821 aaaatctatc acgacagttt tctttccctt ctgggccaaa ccagtggcga tggccgcgct
     2881 ggaggttgtc ttaccaaccc cccctttgcc cgaagtaaca acaataatgc gtgccataga
     2941 aattccttgt taaaaaggga tcaatttaac ggttgaacgg tcaaagcgtt ttcgactaac
     3001 tgcagtcgcg ccgctttgcc ataaaattct gctgggattt gatcactcag ccagtattca
     3061 cctgcgatgg acaccaattc cgccatcagg ttcgtacaaa atatttgcgt ttcccggtca
     3121 ccacttgccc ctgccagcgc acgaccgcgc atcatgccat agacatgaat gttcccatcg
     3181 gcaatcaatt cggccccagc gctaacgtgg cttgtaacaa tcagatcaca ttgtggagca
     3241 taaatacgct gaccggaacg caccggggta tctattaaac gcgtttttgt gaccggcgtg
     3301 gtattttgcg ctggagcctg tggtgcggga gctggacgtg gcgctttttc ctttccttcc
     3361 gtcaggatag gcagccccat tttttcaatt tcggctttaa gttgcgcgtc tttgcagcca
     3421 cttacgccaa taacccgcaa accggttgcc gaaaccgcct tatgcatcgc tgaccagttt
     3481 accgggtctt ccagtgcact gacgttgagt acaacggggg catgttttaa aaatgcgggg
     3541 gcctgagcga ttttgtcttc cagcgcctga tggataacct taggttctgc ctcatgcaga
     3601 tgaaccacag ataaagtgaa gctactgcct ttaagctcga ttggcgtgtt tgacatcctg
     3661 gccttactca attagctatt aatcatcgcc agcgcgcgat gatgttccga agactataag
     3721 gcatgttata gtctggatta tattgaggca agtcaccctc ccatttattc agagtaaaag
     3781 tctattctgt gataaatggc gctgattcat agcttaaaaa atacccttgt caatcaaccc
     3841 attgccgccg tacttttgat tcttcttatt tacgcttcct ttttccgcac cctaatcgtg
     3901 acagcatctc cagacctgtt taaatatcca gcattgaggc aagtgtattt tttcaccacg
     3961 ccaggctttc ttaactttcc attttttgca gcatcattaa agaacagata cccgttatct
     4021 taatatccgc cacagaattg ctctatcata gaaaccgcag aagacgtctg atacttctgt
     4081 caggtcatcg taactcgtcc aggtcagcgg ttttgattac ggattgatga cgaaatgagc
     4141 ataagtgttg tacggttgga ttatcaggat gtgctatgaa tatgatgcgt attatttata
     4201 tcggattgtc aggtgtgggg atgatgttct catcaatggc gtctggacat gacgctggcg
     4261 gacttcaatc tccggcgtgc ggcgttgttt gcgacccgta tatttgtgtg aactcagatg
     4321 gcatttcccc agagttaaca aggaaatatc tcggcgaaaa agccgctgaa aacttacaat
     4381 cattacaagg ctacgatccc agcgaattta cattcgctaa cggtgtattt tgcgatgtta
     4441 aagaaaaatt atgtcgtgat gatcgttatt ttggtgtaga tggtaagcgg agtggaaaaa
     4501 tcaatcaaac caccacaaag atgttattta tgtgtcgtga gtgatgcctt gtttacatta
     4561 tgttctctgc tattcgtcat taacctcttg agcgacagag aggagtgggg cgttaacgtc
     4621 tatgatgggg atagtccccg tcggttgaca tttacttaat ttgaatatcg acgttatatt
     4681 ttttagtttt gtttttacgg gcgtcgtttc gagtttgcag cactcgcagc tcatatttgc
     4741 cagaagcagg gagtgagtat tgtccgtgac tatcgagttc tggggaatat ctggataaat
     4801 caacagaatc atcgattcct ggcccgaaga gataagtatc agcgccttca ttagagatac
     4861 tcacatgcac tttctgacct tttttggcat agaaggtata tgtatcgtaa tcgtatccct
     4921 ttatttcgcc ggagtactga gcgctgctat ggcctttgct gaactcaaca ttgacatttt
     4981 tacccgcagc aaagctcgtg gaggttagca gtgcaagcag caatacagcc ttactgatgc
     5041 tcttgatttt cattggtagt ctcctgttca gaatgagtac gatgaactgt tataatataa
     5101 caatccctta cgggagaatt ccagtggctt ttctgcgttc ggtaaaatcc cggcgcggca
     5161 ttttttgccg gggggattac ttataaaggg gcatggactc aacaaaccgc agaaatctac
     5221 agctggttgg cttgtggcgc gggtttcatt gatgttagct tatgccaaaa cctggcatct
     5281 taaagagtaa aagtatgttt tgtgtgattt atcgaagcag caagcgtgac cagacctatt
     5341 tatatgtcga aaaaaaagac gatttttcgc gtgttcctga ggaactgatg aaaggttttg
     5401 gtcagcctca gttagcgatg attctgccgt tggatgggcg gaagaaactg gtcaatgctg
     5461 atattgaaaa ggttaaactg gcattaaccg agcaaggtta ctatttgcag ttaccgccac
     5521 cacctgaaga tttgctgaag caacatcttt ccgtcatggg gcagaaaaca gacgacacta
     5581 acaaataacc gatatccggc ggtggcatta tctttgtcgg cgcgggtttt catatccacg
     5641 ataaggtgag gggaacgtta tgtatcaaca tcacaactgg caaggtgcgc tgctggatta
     5701 tccggtgagt aaagtagtct gtgttggcag taactatgcc aaacatatta aagagatggg
     5761 cagcgcagtg cccgaagagc cagtgctgtt tattaaacca gaaacggcac tgtgcgatct
     5821 gcggcagcca ttggcgatcc catccgattt cggttcagtt catcatgaag tcgaactggc
     5881 ggtgttgatt ggcgcgacgc tgcgtcaggc aacggaagag catgtccgca aagccattgc
     5941 cggttacggc gtggcgcttg atctgacatt gcgtgatgtt cagggaaaaa tgaagaaagc
     6001 cgggcagccg tgggaaaagg ctaaagcgtt tgataactct tgtccgcttt ccgggtttat
     6061 ccccgcggcg gaatttaccg gcgatccaca aaatacaacg ctgggcctga gcgtaaacgg
     6121 cgaacaacgc cagcaaggta cgactgcgga catgatccat aaaatcgttc cgttgatcgc
     6181 ttatatgagc aagtttttta ccctcaaggc cggtgacgtt gtgttgacag gcacgcctga
     6241 tggcgtcggc ccgttgcaaa gcggtgatga gctgacagtc actttcgatg ggcattcttt
     6301 gacaactcgc gttttgtaat actttttgcc gcctgaaagc ggcggcaaaa cttgcatcgc
     6361 tgtgccagac tggttataag gtgcgtttta acgtaatggc ggaacacctg atgagcgatg
     6421 tacctttctg gcaaagtaaa accctggacg aaatgagcga tgcggaatgg gagtcgttgt
     6481 gtgatggttg cggtcagtgt tgcctgcata aactgatgga tgaagacacc gacgaaatct
     6541 acttcactaa cgtcgcctgt cgccagctca atattaaaac ttgtcaatgt cggaactacg
     6601 aacgtcgttt cgagtttgaa cccgactgca ttaaattaac ccgtgaaaat ctgccaacat
     6661 tcgaatggct gccaatgatc tgcgcttatc gtttgctggc ggaaggtaaa gatttacctg
     6721 cgtggcatcc gctacttact ggttcgaaag cggcaatgca tggtgaacgt atctctgtgc
     6781 gtcatatcgc agtgaaagaa tcagaagtga ttgactggca ggatcatatc ttaaataaac
     6841 ctgactgggc gcagtgataa atttaataga tgttgatggg ggctttctta atatacgtct
     6901 ggatgctcgc catttcgtag ccctgttttt gaaagcgctc tgtcataatg agagttaccc
     6961 gataccttat tatcaacagg taactctcaa tctttaaaaa acgtctgtgt ttttaaatgt
     7021 gataaacctt ataccttgag tacatggaga gaataatcgc tgatcagact tcaggtacct
     7081 caaagagtgt ctttttaccg tgttttttct gatactcatt acaggtgtta atcatttttt
     7141 tggccgcttc ttttagcaaa gaaagcatta aatcatcata atcaacgtag aatctggtgg
     7201 tttcagtttc cgtttttatc tctccgatgg cggctatttc ggtggttaat ttcaatttgg
     7261 cggcatcgat atctttattc gcttgtttaa ccgtgttaga cagggtggta aagaaactct
     7321 gcacagattt taacttgttc ttcaattctg ggatcagttt cccttcaact acgcccgcag
     7381 caatagaata ggaaatgatt aaaccaaatg gaccggcgac gacaccggct gcggcaccgg
     7441 catacgcttc cttcctgatt ttatctacct gtgactggaa atagctgctt ttttctgaaa
     7501 aatcattggt taactggcta tctaacgcca gcagtttccc ggaagcgttg ttgaaacttt
     7561 gtgagcttac cagcagggat ttttgcgctt cattcagctt cgtgatgccg tcatccagta
     7621 ccttaatgag aatgtctttc tgggcggatg ctttcttctc attgtactca tcaaatagca
     7681 aaatatacgc agcgagcaat tgcgtcgcaa caccacacca ttcatacact gtttgggttg
     7741 cttcaaaata cttatcctgg ctatccataa gtaaggtttt aatatcgccg actaaaacgg
     7801 aggctgcctg tgaatactcc tgtttaaagc gacttaactc ttttatggtt tcatcaaagg
     7861 tctgccaggg gatgacctga tcgagatatt tattataaag atctaatgct ccatctgcgg
     7921 tttcgattgc gtttttaact acttctaccg ttttatctgc aacgatttca gtcataatca
//
LOCUS       DAZPDU010000188         7953 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZPDU010000188 DAZPDU010000000
VERSION     DAZPDU010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7953
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.190"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(125..388)
                     /gene="cedA"
                     /locus_tag="ACHURS_003788"
     CDS             complement(125..388)
                     /gene="cedA"
                     /locus_tag="ACHURS_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFR1239414.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            571..2832
                     /gene="katE"
                     /locus_tag="ACHURS_003789"
     CDS             571..2832
                     /gene="katE"
                     /locus_tag="ACHURS_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFR1239415.1"
                     /translation="MSQHNEKNLHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(3090..3848)
                     /gene="chbG"
                     /locus_tag="ACHURS_003790"
     CDS             complement(3090..3848)
                     /gene="chbG"
                     /locus_tag="ACHURS_003790"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFR1239416.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMYFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNSG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDRSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGSYRDV"
     gene            complement(3861..5213)
                     /gene="celF"
                     /locus_tag="ACHURS_003791"
                     /gene_synonym="chbF"
     CDS             complement(3861..5213)
                     /gene="celF"
                     /locus_tag="ACHURS_003791"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFR1239417.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5318..6160)
                     /gene="chbR"
                     /locus_tag="ACHURS_003792"
     CDS             complement(5318..6160)
                     /gene="chbR"
                     /locus_tag="ACHURS_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFR1239418.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(6168..6518)
                     /gene="chbA"
                     /locus_tag="ACHURS_003793"
     CDS             complement(6168..6518)
                     /gene="chbA"
                     /locus_tag="ACHURS_003793"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFR1239419.1"
                     /translation="MMDLNNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6569..7927)
                     /gene="chbC"
                     /locus_tag="ACHURS_003794"
     CDS             complement(6569..7927)
                     /gene="chbC"
                     /locus_tag="ACHURS_003794"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFR1239420.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGAFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYVVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEEREEDIANAL
                     KF"
BASE COUNT         2134 a         2082 c         1780 g         1957 t
ORIGIN      
        1 acttatttat agcaatagct cgtcaaaggc agctttttgt tacaggtggt ttgaatgaat
       61 gtagcaacga aatgcagaat ttcaggtcat gtaactcccg gcaaaaccgg gaagtatgta
      121 atccttactc agtcacttcc ccttcctggc ggatctgatt tgcccaacgt tgggcagatt
      181 caggcacagt aaacgccggt gagcgcagaa atgactctcc catcagtaca aacgcaacat
      241 atttgccacg cagcatccag acatcacgaa acgaatccat ctttatcgca tgttctggcg
      301 gcgcgggttc cgtgcgtggg acatagctaa taatctggcg gttttgctgg cggagcggtt
      361 tcttcattac tggcttcact aaacgcatat taaaaattag aaaaactgta gtttagccga
      421 tttagcccct gtacgtcccg ctttgcgtgt atttcataac accgtttcca gaatagtctc
      481 cgaagcggga tctggctggt ggtctatagt tagagagttt tttgaccaaa acagcggccc
      541 tttcagtaat aaattaagga gacgagttca atgtcgcaac ataacgaaaa gaacctacat
      601 cagcaccagt caccactaca cgattccagc gaagcgaaac cggggatgga ctcactggca
      661 cctgaggacg gatctcatcg tccagcggct gaaccaacac cgccaggtgc acaacctacc
      721 gcccctggga gcctgaaagc ccctgataca cgtaacgaaa aacttaattc tctggaagac
      781 gtacgcaaag gcagtgaaaa ttatgcgctg accactaatc agggcgtgcg catcgccgat
      841 gatcaaaact cactgcgtgc cggtaaccgt ggtccaacgc tgctggaaga ttttattctg
      901 cgcgagaaaa tcacccactt tgaccatgag cgcatcccgg aacgtattgt tcatgcacgc
      961 ggatcagcag ctcacggtta tttccagcca tataaaagct taagcgatat caccaaagcg
     1021 gatttcctct cagatccgaa caaaatcacc ccagtatttg ttaggttctc taccgttcag
     1081 ggtggcgctg gctctgcaga taccgtgcgt gatatccgtg gttttgccac caagttctat
     1141 actgaagagg gtatttttga cctcgttggc aataacacgc caatcttctt tatccaggat
     1201 gcgcataaat tccccgattt tgttcatgcg gtaaaaccag aaccgcactg ggcaattcca
     1261 caagggcaaa gtgcccacga tactttctgg gattatgttt ctctgcaacc tgaaactctg
     1321 cacaacgtga tgtgggcgat gtcggatcgc ggcatccccc gcagttaccg caccatggaa
     1381 ggcttcggta ttcacacctt ccgcctgatt aatgccgaag ggaaggcaac gtttgtacgt
     1441 ttccactgga aaccactggc aggtaaagcc tcactcgttt gggatgaagc acaaaaactc
     1501 accggacgtg acccggactt ccaccgccgc gagttgtggg aagccattga agcaggcgat
     1561 tttccggaat acgaactggg cttccagttg attcctgaag aagatgaatt caagttcgac
     1621 ttcgatcttc tcgatccaac caaactgatc ccggaagaac tggtgcccgt tcagcgtgtc
     1681 ggcaaaatgg tgctcaatcg caatccggat aacttctttg ccgaaaacga acaagcagca
     1741 ttccatccag gtcatattgt tcctggtctg gacttcacca acgatccgct gttgcaggga
     1801 cgtttgttct cctataccga tacacaaatc agtcgtcttg gtgggccgaa tttccatgag
     1861 attccgatta accgtccgac ctgcccttac cacaacttcc agcgtgatgg catgcatcgt
     1921 atggggatcg acactaaccc ggcgaattac gaaccgaact cgatcaacga taactggccg
     1981 cgcgaaacac cgccgggacc gaaacgcggc ggttttgaat catatcagga acgcgtggaa
     2041 ggaaataaag ttcgtgagcg cagtccatcg tttggcgaat attattccca tccgcgtctg
     2101 ttctggctaa gtcagacgcc attcgagcag cgccatattg tcgatggttt cagttttgag
     2161 ttaagcaaag tcgttcgtcc gtatattcgt gagcgcgttg ttgaccagct ggcgcatatt
     2221 gatctcactc tggcccaggc ggtggcgaaa aatctcggta tcgaactgac tgacgaccag
     2281 ctgaatatca ccccacctcc ggacgtcaac ggtctgaaaa aggatccatc cttaagtttg
     2341 tacgccattc ctgacggtga tgtgaaaggt cgcgtggtag cgattttact taatgatgaa
     2401 gtgagatcgg cagaccttct ggccattctc aaggccctga aggccaaagg cgttcatgcc
     2461 aaactgctct actcccgaat gggtgaagtg actgcggatg acggtacggt gttgcctata
     2521 gccgctacct ttgccggtgc accttcgctg acggtcgatg cggtcattgt cccttgcggc
     2581 aatatcgcgg atatcgctga caacggcgat gccaactact acctgatgga agcctacaaa
     2641 caccttaaac cgattgcgct ggcgggagac gcgcgcaagt ttaaagcaac aatcaaggtc
     2701 gctgaccagg gtgaagaagg gattgtggaa gctgacagcg ctgacggtag ttttatggat
     2761 gaactgctaa cgctgatggc agcacaccgc gtgtggtcac gcattcctaa gattgacaaa
     2821 attcctgcct gatgggaggc ggcgcaattg cgccgcctca aatgatttac atagtgcgct
     2881 ttgtttatgc cggatgcggc gtgaacgcct tatccggcct acaaaactgt gcaaattcaa
     2941 tatattgcag gaaacacgta ggcctgataa gcgaagcgca tcaggcagtt ttgcgtttgt
     3001 cagcagtctc aagcggcgca gttacgccgc ctttgtagga attaatcgcc ggatgcaagg
     3061 ttcacgccgc atctggcaaa catcctcact tacacatccc gataactccc caaccgataa
     3121 ccacgctgag cgatagcacc tttcaacgac gctgatgtca acacatccag ctccgttaag
     3181 cgtgggaaac agtaagcact ctgacggata gtattatcga taaacgccgg atgacacatc
     3241 acctccagcg aacgatcacc ccgatggctt gcgtcatcca gcacttgcag gaacagtgcc
     3301 tcgctaatct cttcaccata aaacgcgcta ctgaatccct gagaactgcg cagattagcc
     3361 ggtaaatcac cagagttaga tagcggctga cggtcgatgc gcaacgcaat cccctcttca
     3421 gccgcaaacc tggcaacaat cgggaaaatc tgcgggaaca tatgcacatg atgatggcta
     3481 tcaagatgcg taggtttgcg tccaaatagc tcaatgaaac gcaaatactg actggcaagc
     3541 tcctgagtaa tttcttccag cggtaaagca tcttcttctg ccaactgcca gatccatttt
     3601 cccagcacac catcgcgggt taaccccggc atagctgtca gtggcttgcc catagtaagg
     3661 acaaagtaca tccctatggc cagactcggt tcatcacgac tcaactgcac cgcatggtca
     3721 atagcctgcc cattcaccag cgccgtcgtc gatgtgacaa tcccattgcg acaggcctcg
     3781 ataatgccgt agttctgacc tttgcttaag ccaaaatcat cggcattaac aatcagtaag
     3841 cgttccataa tcagcctcgg ttaatgtgct tttttaagct ctgcgatgca gtcggcaaaa
     3901 tttggcagcc atttctcgtg cgccagaatc atctcgcgtg ccagcagctc agcatcgcga
     3961 tcggaatgca ccaacggact aaggtttagc gccagtaaca catcgttaaa ttctccgcta
     4021 agtgcggcgt tgctggcagc aatctcgaag cctttaatgg tgtgaatcag ccccatcact
     4081 ttatcatcga aatgcgtaat gcgcggatgt ggcgtcgcgc catcgcgccc cagcttacag
     4141 gtcatttcta ccgcccagtc tgccggaata ttatcaatat gcccatgatg cgggatatta
     4201 acgtaatgtt cagcttgctt gtcgttgtag atagcgttga tcacttcgca cgctgcatca
     4261 gagtaataag ccccaccgcg ctgttccagt tctttcggct taactttcaa ctccgggttt
     4321 ttatacagct caaaaagttg tttctcgact ttctgtacta cctgcgctcg tgcgccgcct
     4381 ttgtagtatt cgcccatttc aatagccagc atctcttttt gcttgaagta ataaagcaaa
     4441 taagaacacg gcaacagatt cagagaacga attaagcctt cgctaaatgg cagatcgaaa
     4501 atatttttaa cgccagatgc ttttaactgc ccggatgcca caccatcaag caattcggca
     4561 aagcgcgact tgccatttac cagcacatcc ttaatgaaca ccatatggtt gaggccgaac
     4621 aaatcgatag ataaatcatc gctgtctttc agcatcagaa catcgtgaat aaacatcttc
     4681 atgccgatcg gaatattaca cacgccgata aagcgtttaa atccggtatg acgataaacg
     4741 gcttcagtaa ccattcccgc cgggttagtg aagttaatca cccatgcatt cggacaaagt
     4801 tcttcgacat cttttacgat gtcaaaaatc accggaatgg tacgcagacc tttaaacaga
     4861 ccgcccgcgc cgttggtttc ctggccaaga taaccatgac ttaatggaat acgttcatcc
     4921 agttcgcgcg ccggtaattg gcctacgcgc agttgggtag taacgaaatc agcatctttc
     4981 aatgcttcgc ggcgatccag cgttttataa agcttcatcg ggacgccagc gttatcaatc
     5041 atccgttggc agagttcaaa aatgatatcc agtttctctt taccatcttc gacatccacc
     5101 agccataatt cgctgaccgg caattcgtga taacgcttaa taaatccttc cagtaactcc
     5161 ggggtatagc tgctcccgcc accaatagtg acgactttta atttctggct cataatttct
     5221 cccttcagta cagaatactg atatctggca tatctgcccc cccggacata aataatccag
     5281 caacaggaca gatatgtgaa ttgtcaggta taacgactta ctgattaaat tcagtcaatt
     5341 tcttacgata gctcttaggc gtaaaggacg ttaatttctt aaacgtttta ataaacaaac
     5401 tcgggctact ataaccggcc tcaaacgcaa tatccgtcac ggaatagttg gtcatttcca
     5461 gttgtttttt ggcaaaatta atacggatct cattaataat ctgcattggt gttttgccat
     5521 aatatcgttg agtcgctcgc gtcaaatatt cctgtgattt ggctgacaac gtcaccatat
     5581 tctccagcgc cgattcacta aactgctctt tatcatgcat cttttctacc gtacttttca
     5641 gccactgcgg tacatcatca atcacctgtt cttcgcggta atgacgtaaa cggttaatga
     5701 cataaaaagt aaccatctca acaaactctt ccagccctgt ttcgcggaaa ttcaatgaag
     5761 aaatcactgt ttccacatag gtgagaaaag cgttattggt ccggtatacc tgcgaagcga
     5821 caaagcaata aggcaacaac ggcaggtaat gctgctcaaa aaagcgttta ctgatcccaa
     5881 cgttcaatat acgcgtggca ccaaactcat aaaaactttg atggtgcgaa cctaacggaa
     5941 taaaaacaaa atcgccccgt tccagtaaca cgcgcttacc gttaatctcc tggaaataac
     6001 gcccggttaa taccagagta aattcataat agtcgtgctg atgcagcccg ctgatactct
     6061 cagttttgtt atagataaac acatggaagt ttttgccatt aaacaactgc tgttctcggg
     6121 cagtggcaat ttccggcgcg ttaatcactg gctgcatcat cgactcctta tgccttcagt
     6181 ttttcatgaa gctcaattaa ttcagtaatc agttcacgcg caagcatgga cgtcattaaa
     6241 tgatcctgag cgtggaccag caccagactc actttcatct taccttcgcc cgcatcgcct
     6301 tcaatcagtt tcgtctgtac cagatgcgct tcattcaatg ccatccgtga ctgatccatc
     6361 atggcttttg ctgcagcaaa atcgccctgc ttcgcctgtt tcagtgccgc atacgccagg
     6421 ctgcgcgctt gtccggagtt gatgataagc cccatcacca cttcttccag ctcttcagct
     6481 tccgtttgcg tatcgggaat gttattgaga tccatcatac atcgttcctc tctattctta
     6541 ccggcacgat tacccgtacc ggcatcgatt aaaatttcag ggcgttagcg atatcttctt
     6601 cgcgctcttc tttatcaatc gcgttttgtg ctttgttagc caccacaaca aagggcagat
     6661 aaattaacgt tgcgatacca aggttgaaga gtgcaaccag caatgcggcg acgctaccgt
     6721 tggtgttaaa gaaggctccc agaccggttg gcatggtcca cggtgcaata ttggtcaccg
     6781 gaggaatgat gcccatgtag tacgctgcga gggtgattgc cgccagaatc ggttgtacca
     6841 gtacaaacgg gataaacatc accgggttca tgataattgg cagaccaaac agaatcggtt
     6901 cgttaatctg gaagatgccg gacggcagcg ccagttttgc cacctgacga taatcagcac
     6961 ggcgagaggc gataaagata gccaggatca ggcctaaagt cgcaccactg cccccaagga
     7021 aaataaagga gtccagcatc ggcttggccc agatatggaa ggtcttaccg gctgccagcg
     7081 ccgcttcgac ggaaccatat tgctgatagg tcgcgatatt ttccagcgcc cacggcgtca
     7141 taatgccgtt gtccagtgcg gtcagcgcca gcgcgccatg aataccgaag aaccagagca
     7201 gtggaacaaa gatcacatag gcccagccca ccacgctacc caacgatgcc agtggggttg
     7261 agatggtatc cataatgatc tgatggaagt tggtgcccca ggtattcaac gcccaggcaa
     7321 taatccccat cacggaaaga ataataaagc cgggaattaa tgccgagaag gaacgcgata
     7381 ctgaagcagg tacgctatcg ggcaatttaa tgacccaatt gcggcggaca ataaaggtaa
     7441 acatttctgc caccaccagg ccaataataa tcccggagat gatattcgct ccacctaacc
     7501 agtttgcgcc aaccacatag gcctcaccga cactatatgg ggtgacggtc ataaatgctg
     7561 caacggataa caacccagcc gccagcgcat cgactttacg ctcttctgcc agcgccatgc
     7621 caataaagaa cggtgccatt aaagacatta tccctaatgt tccgttatat acgttgccgc
     7681 caataccttt caggccatta agtgtttcaa tggttgaggc gtcgaggcga atacctaagg
     7741 aataaaaaaa cgccccctcc ccaaagctta gaaaaacgtt gttaattaat acaaacatgg
     7801 cccccgcaag ggttaacggc attaagcgaa taaagccatt tttgattgca ttaacgtgtg
     7861 gctgctttcc tattttaact gcaaaaggga ggagtacctt ttcaagcgat gcaataacat
     7921 tactcataga aaaataccct taaaaaccgc aat
//
LOCUS       DAZPDU010000189         7946 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZPDU010000189 DAZPDU010000000
VERSION     DAZPDU010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7946
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.191"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            321..437
                     /locus_tag="ACHURS_003795"
                     /pseudo
     CDS             321..437
                     /locus_tag="ACHURS_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904967.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            812..1054
                     /gene="yiiF"
                     /locus_tag="ACHURS_003796"
     CDS             812..1054
                     /gene="yiiF"
                     /locus_tag="ACHURS_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HFR1239421.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(1371..2300)
                     /gene="fdhE"
                     /locus_tag="ACHURS_003797"
     CDS             complement(1371..2300)
                     /gene="fdhE"
                     /locus_tag="ACHURS_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFR1239422.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(2297..2932)
                     /gene="fdoI"
                     /locus_tag="ACHURS_003798"
     CDS             complement(2297..2932)
                     /gene="fdoI"
                     /locus_tag="ACHURS_003798"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFR1239423.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2929..3831)
                     /gene="fdoH"
                     /locus_tag="ACHURS_003799"
     CDS             complement(2929..3831)
                     /gene="fdoH"
                     /locus_tag="ACHURS_003799"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFR1239424.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3844..6894)
                     /gene="fdnG"
                     /locus_tag="ACHURS_003800"
     CDS             complement(3844..6894)
                     /gene="fdnG"
                     /locus_tag="ACHURS_003800"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(6307..6309),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFR1239425.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            7088..7921
                     /gene="fdhD"
                     /locus_tag="ACHURS_003801"
     CDS             7088..7921
                     /gene="fdhD"
                     /locus_tag="ACHURS_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFR1239426.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1839 a         2055 c         2070 g         1982 t
ORIGIN      
        1 atttacatca ttccctgtct atgccggtaa tattgaataa gaactctata aatttttatt
       61 cttcgttggc accatacgtg tcttgctaca tcactgttca ggagcattta aatattattt
      121 gtagagctga gtggcaatta tagcagataa tgcttaactc atcatcttag ttcaggtttg
      181 agcgattttt gatagtcaaa acctcctctg gtgccatact tttctcataa atatttttaa
      241 aatctatagc tcacaaacat tatgcaaaga taatgggctg gcatcattgt cagtatcatt
      301 catttactgc tctgtatgtt atgaatcatt atgttgtatg tttgctctta aaccgtggga
      361 aggaggctat aatggttgcc aatattaatc tcattaaaca agaaagttat tctgttgtta
      421 atttagaaaa acaactttcc cgaactgtta ccaacaaaat tataacgatg agcaagtaaa
      481 gaccggttgg tggcagtact gaccggtctg tgatgaaaga ttaatttcac cttaaatcct
      541 ggtgctgtta ggatgctgac gtttttcggc tacaccattc agattatgca aataaatatc
      601 cctgaatata atatattcat agcacccata actcatcctg ggcgataact aaaccatttt
      661 tgagtaataa atgaaacaac tgcaacgctc ttattatttc tggaaaacgc ctcgcaaaaa
      721 taaaaaatga tgcgtaaatg agctttcttt aaagattcgg caagggtact tttctctcca
      781 tagattcaaa aatgatattg gcgagatatt tatgaactct ctggcaggta tagatatggg
      841 cagaatttta ctcgatttat cgaatgaggt gattaagcaa cttgatgatc tcgaggtgca
      901 gcgtaatctt cctcgtgcgg acctattaag ggaagcggta gatcaatacc tgataaatca
      961 atcgcaaaca gcaagaacca gtgttcctgg catctggcaa gggtgtgagg aagatggtgt
     1021 cgaatatcag cgtaagctgc gcgaggaatg gtaatgggaa aaaatgatgt caatcagatt
     1081 gctgacaacg tgcgcgttgt tcatgccgga tgcggcgtga acgccttatc cggcctacaa
     1141 agtcatgcaa attcaatata ttgaagttta ttggtaggcc tgataagcgc agcgcatcag
     1201 gcaatcatac gcttgtcatc agtgacatac ccataacctt atcgatgtgt tggagggaag
     1261 aacgaggcaa acagacaatt gcggcatgat cttgaaatac gagagatggt gtcgtcacac
     1321 cctatgagct gtaatccggg cgacctgcgc cacccggaaa acgtcagaga ttactctcct
     1381 tcacccggaa acaggaacgg gttaatggaa ctacgggcgt agccttcttg ctccattcgc
     1441 gcgtccagta ccagagaggc gaggtcatct gccacggctt caactttcgg ttctttttcc
     1501 tgataaagaa tcttcaggta agtgccgcag tcatcgcagc tttcggcttt aatcgcggcc
     1561 tgttcgtcat ccagcgacca gtaatgcagt ttgccgctct gctcgcagtt gctgcatttt
     1621 acgcgcacta cgtgccattc ggtttcacac aggttgcagt gcaggtaacg cagaccctga
     1681 gtggtgccaa tttgcaccat gctggacacc ggcatagagc cacataccgg gcaatattga
     1741 cgttgttcgc cgtattcagc gcgggctttg ccggggatca gattggccat ctgcgcccag
     1801 tagagcgaca gcgcagccca gataaacggc gctttatcgc tgctgacgga cgagaaatca
     1861 gaggcaaaca gcgcgctggc catatcttcc agctcctgag tcgatgcctt ctccagattc
     1921 tcaatcactg ccagcgccgg gccgctcatt tcaggtttca gctcagcaat tagcgccatc
     1981 agcagctttt gccagtgctt atcacgcggc agaacgtgaa tatccagcgg gggcttgccc
     2041 tgtgcgctgg cttctttaat gcgcgcggtc agatccatct ccagcggatg gtcgtacagc
     2101 accacttcct gggcgtgggc gataagcgca gcaaagcgca ggtaatcacc cagcggatta
     2161 ttttctgcca gctcgcgcag acgctcggcg cggcggttgt ataaattctt gagccgaggg
     2221 aacaataacg gcggaatcat atccgccgta cgtttctcgc tcgaacccag ctcatcttgc
     2281 gggattatgc gaatactcat tcagctttct tttccgttgt cttgcggacc tcacggtacc
     2341 agcgcgggtg atgtttcttc gcccaggcgc tggtaaccca tccttccacc atggcggtaa
     2401 tcgtgccttt cacccaaagg gcggcgtaga tatgcaccat gataaccaca attaacgcca
     2461 ctgcggcaaa tgaatgcagc attaacgcga atcggatcac cgggattgag aaagcaggcg
     2521 caaaataagg acgccagata atcacgccgc tcaccagcag cagaaccagg aaaataatcg
     2581 ccgcccagaa aacgcatttc tgaccgaagt tataacgccc ggtgtcacct acttcctcgt
     2641 tgacgacgat cttacgaata ttcttcgccc aaaagatatc atcccgattg attaggttgt
     2701 gatgccagta acggaaaaac atgatgatga acgaggcaaa cataaccacg ccgacaaacg
     2761 ggtgcagaat tcgcgccagc tgcggtgtgc ccatgatttg catcaaccag ttgaaggacg
     2821 ggaacaaaaa gcccagcccg ctcaccgccg ccaggatgaa gcagaaggcg gtgatccagt
     2881 ggttgatacg ttccggcgcg gtgtagcgca cgatggtgtc acgtcgtttc atttgcgctc
     2941 ctcgtctttc tcttcgtgca gattattctc ttcctcatcc gcacggttcg gaccgacacc
     3001 cacgtagtgg aagatactgg ccgcaaaggt agccgcaaag ccaacagccg cgagcggttt
     3061 ccagatgcct ttccagaatt tcacggtttc gctgatttcc gggttctccg gcaagccatg
     3121 atacagattt ggcttgtcag catggtgcag cacgtacatg acgtgtgtac caccgacgcc
     3181 tgccggatcg tacagacccg cattgtcgta accgcgggtt ttcagctcag caacgcgctc
     3241 gctcgccagc gttttcatcg actctttcgt accgaagtga atcgcgcccg ttgggcaggt
     3301 cttcacgcag gctggttctt gcccaaccac cacgcggtca acgcacagcg tacatttgta
     3361 gacgcggttg tcttccgggt tgaggcgcgg aatgtcgaac ggacagcccg caatgcaata
     3421 accgcagcca atgcactgct cggactggaa gtcgacgata ccgttggcat actgaataat
     3481 tgcaccttcc gccgggcacg ctttcaggca gcctggatcg gaacagtgca tacagccgtc
     3541 tttgcggatc agccattcca gtttgtcgtt ctgctccact tccgagaagc gcatcaccgt
     3601 ccacgatttg gcgcttaaat cattggggtt gtcgtacacc ccaatgttat tgccgacggt
     3661 atcgcgaatg tcgttccact ctgaacacgc cacctgacag gctttacagc cgatacaggt
     3721 ggtgacgtcg atgagtttcg ccacttcttc ctggaagtcc cgcgcctgag gcgcgggggt
     3781 cagaccgtta gtcgcggaac gacgaatgat atcttgcgat tgataagcca tatgtcgtct
     3841 ccgttacacc ttttccacat tcacaaggaa ggacttaaac tccggcgtct gcgtgttcgc
     3901 atcaccaacg aatggcgtta acgtattggc aataaagcct tttttagcta cgccttcata
     3961 gccccagtga atcggaatac cgatggtatc gatatctttg ccgttcgctt tcagcgtgcg
     4021 aatacgtttg gtcaccaccg ctttggcttt gatatagcca cggttggagg agactttcac
     4081 ggtatcgccc tgggcaatgc caagtttatt cgccagcgac tccccgattt ccacaaactg
     4141 ctctggttgc aaaatcgcgt tcaacagcgc gtgtttggtc cagtagtgga agtgctcggt
     4201 cagacgatag gtggttccga catacgggaa cttatcggct ttacccaatg cttcggcgtc
     4261 gtctttaaag attcgcgcag ccgggttcga gataacgttt ggatgcagcg ggttagttcc
     4321 cagcggcgtt tcaaacggct cgtagtgttc cgggaacgga ccttccgcca tcttatcgag
     4381 ggcaaacaga cgccccatgc cttcctgctg catgataaac ggcccgacgc cgctgcccgg
     4441 aggcgctgcg ctgtagtccg gaatatccca gccggtccac ttagtgccgt cccatttcag
     4501 caactgacgc ttcggatccc acgggttacc ctgcggatct gcggaggcgc ggttatacag
     4561 aatgcggcgg ttaagcggcc atgcccatgc ccagcccagc gtgttaccga ggccagacgg
     4621 atcggcgtta tcacgacgcg ccatctggtt gccttccggc gtccagctac cggcgaaaat
     4681 ccagcagcca caggaggttg taccgtcatc gcgcaattgg gcgaacgagc taagttgttg
     4741 gcctttcttg acgataaccg caccggttgc cggatcggta atatcggcca gcgccttacc
     4801 gttgctctcc atcgccactt cttccgactt cggctcatgc ggaatggcgt agttccaggt
     4861 catgttcagc acctgatccg ggtttgcgcc accctgttcg gcatacatct tgcgcaagcg
     4921 caggaagata ccggagagga tctcgccatc agtcagcgca atccctgggg cgtccgcacc
     4981 tttccagtgc cactgcaacc agcgacctga gttaacgatt gaaccgttct cttctgcgaa
     5041 gcaggtcgat ggcagacgga acacttcggt ctggatcttc gacgagtcaa cttcgttcag
     5101 ctcaccgtgg ttctgccaga agttagaggt ttcagtgttc agcgggtcga tggtgacgag
     5161 gaacttcagt ttcgacagac aaccgatcac tttgtttttg ttcgggaatg aggcaacagg
     5221 gttaaagccc tggcagatat agccattgac cttgccctct ttcatcatct cgaagtattg
     5281 caggacgtcg tagcctttat cccacttcgg caaccagtca aagccccagc tattttccgc
     5341 cgtcgcttta tcaccaaaga aggccttcat catagagacg aagaatttcg ggtagttgcc
     5401 ccagtagttt acctggcctt ccagcagcgg ttttggcgtg ttggcggtaa ggtaggtttg
     5461 cagatcggtc tgcttctcgc ttggcagcgt catgtaacct ggcaggctct gcgacagcag
     5521 ccccaggtcc gtcagcccct gaatattgga gtgaccgcgc agggcgttaa cgccgccgcc
     5581 tgccatcccc atattgccga gcagcagctg gatcatcgcc atcgtacgaa tgttttgcgc
     5641 accaacggag tgttgcgtcc agccgagggc atacaggaac gaggcagttt tatcgtgagc
     5701 actggtttct gcgatgtatt cgcagacttt caggaacgcg tcttttggcg taccacagat
     5761 gttttcaacc acatctggcg tgtagcggga aacgtgctgt ttcagcaagt tccacacgca
     5821 gcgcggatgt tgcagcgtgg tatcgcgttt ggcgaagccg ttttcgtcca gttcgtaagt
     5881 ccaggaggat ttatcgtact tgcgtttttc cgcgtcatag ccggtgaaca ggccatcttc
     5941 aaagccgtaa tcctcacgca cgatcaggct ggcgttggta taggcttcgg tgtattcgcg
     6001 gttgaatttt tcattgttca gcaggtacag caatacgcct gacaggaaag caatgtcagt
     6061 accggaacga ataggggcat agtagtcagc caccgccgcc gtacgcgtaa agcgaggatc
     6121 gatcacaatc agcttcgcgc cattgtgaat tttggcttcc atcgcccagc ggaacccgac
     6181 cgggtgagct tcagcggcgt taccgcccat caccacgacg aggttggcgt tcttgatgtc
     6241 gacccagtgg ttggtcatcg caccgcgacc aaatgttgga gcaagacttg ctaccgttgg
     6301 tccgtgtcag acacgcgcct ggttgtcgac cgcgagcata cccagcgcgc gggagaattt
     6361 ttgcgttaaa tagccggttt cgttactcga cgcggaagca cacagcatcc cggtggagag
     6421 ccagcggtta acagtcacgc cttcggcgtt ttgcgcaatg tagttagcat cgcggtcttc
     6481 tttcatcagt ttggcgatgc gatcaaacgc ctcttcccaa ctgatttgct gccatttatc
     6541 agaacctggc gcacggtatt ccggaaactt cagacggctt tcggagtgga tgaaatccac
     6601 caggccagcg cctttcggac aaagtgcacc gcggttgacc gggtgatccg gatcgccttc
     6661 gatatggaag atagatgctt tggcgttttt tgctccgtca ccgaggctgt acatcaacag
     6721 cccacagcct acggaacaat aggtgcaggt attacgggtt tcgcgggtgc gcagcagttt
     6781 atactgccgg gtttccgcga gtgctacgct gggtgcaaaa cccagtgccg ctgccgtggt
     6841 gcctgccata ccgccagcgc agatcttaaa gaactgcctt ctgctgacct gcatggattg
     6901 ctccttgttt cgacattgtc acgtcccatt tacattcgct tgctgcgtgt gcagggagtg
     6961 ggagttattt ttctttgcgg aaggggccgc aaaggtccag aattggctca atttccctcc
     7021 atccaggaag ggtttgtaac agaataccat aatgttggtg tgtgtgttct tatctggtta
     7081 agagaaagtg aaaaaaacac agcgaaaaga aatcgaaaat gtgacaaata tcacaggtgt
     7141 tcgtcaaatt gagttatggc ggcgtgatga tttacaacac ccacggttag atgaggtcgc
     7201 ggaagaagtt cccgttgcgc tggtctacaa cggcatttcg catgtggtga tgatggcgtc
     7261 gcccaaagac cttgagtact ttgcgctcgg tttttcgctt tccgagggga taatagaaag
     7321 tccgcgcgat atcttcggca tggatgtcgt tccttcctgt aatggtcttg aagtacaaat
     7381 tgagctttcc agccgccgct ttatggggtt gaaggagcgc cgccgggcgc tggcgggacg
     7441 tacgggctgt ggcgtatgcg gcgtggagca acttaatgac atcggaaaac cggtgcagcc
     7501 gctaccgttc acccagacgt ttgatctcaa caaactggat gatgcattac gtcatctcaa
     7561 cgattttcag ccagtggggc aactgactgg ttgtactcac gccgctgcct ggatgttgcc
     7621 atctggcgaa ctggtcggcg ggcatgaaga cgtgggtcgc catgtggcgc tggacaaact
     7681 gttaggccgc cggtcacaag aaggggaaag ctggcagcaa ggtgcggtac tggtttccag
     7741 tcgtgccagt tatgaaatgg tgcaaaagtc ggcgatgtgc ggcgtagaga ttttgtttgc
     7801 ggtgtctgcc gcgaccacgc ttgctgtaga agtggctgag cgctgtaatc tgacactggt
     7861 aggtttttgt aaaccgggtc gggcaacggt ttatacccat ccgcagcgtt tgagcaatta
     7921 attaaaataa gtcctgcgat atatat
//
LOCUS       DAZPDU010000190         7945 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZPDU010000190 DAZPDU010000000
VERSION     DAZPDU010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7945
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.192"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..359
                     /locus_tag="ACHURS_003802"
     CDS             <1..359
                     /locus_tag="ACHURS_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="acts in anaerobic beta-oxidation of fatty acids;
                     possible ligase/synthase involved in anaerobic growth on
                     fatty acids; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclohexanecarboxylate-CoA ligase"
                     /protein_id="HFR1239427.1"
                     /translation="GRKKDIIVRGGENISSREVEDILLQHPKIHDACVVAMPDERLGE
                     RSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKYPEHIVVIEKLPRTASGKIQKFLLR
                     KDIMRRLTQDVCEEIE"
     gene            complement(416..2794)
                     /gene="ppsA"
                     /locus_tag="ACHURS_003803"
     CDS             complement(416..2794)
                     /gene="ppsA"
                     /locus_tag="ACHURS_003803"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFR1239428.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHEISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVEMMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            3127..3960
                     /gene="ppsR"
                     /locus_tag="ACHURS_003804"
     CDS             3127..3960
                     /gene="ppsR"
                     /locus_tag="ACHURS_003804"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFR1239429.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            4117..5163
                     /gene="aroH"
                     /locus_tag="ACHURS_003805"
     CDS             4117..5163
                     /gene="aroH"
                     /locus_tag="ACHURS_003805"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFR1239430.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGAATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYAPRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLLEGTQKIVGSQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            5295..5486
                     /gene="hemP"
                     /locus_tag="ACHURS_003806"
     CDS             5295..5486
                     /gene="hemP"
                     /locus_tag="ACHURS_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFR1239431.1"
                     /translation="MRYTDSRKLTPETDANHKAASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(5490..6926)
                     /gene="selO"
                     /locus_tag="ACHURS_003807"
     CDS             complement(5490..6926)
                     /gene="selO"
                     /locus_tag="ACHURS_003807"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFR1239432.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGEALLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TAEPGAMLMRVAPSHLRFGHFEHFYYRRESEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FITEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSTASPLRDEFIDRAAFDGW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHGAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(6989..7702)
                     /gene="ydiV"
                     /locus_tag="ACHURS_003808"
     CDS             complement(6989..7702)
                     /gene="ydiV"
                     /locus_tag="ACHURS_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFR1239433.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
BASE COUNT         2030 a         2086 c         1888 g         1941 t
ORIGIN      
        1 ccggacgcaa aaaagatatt attgtccgcg gcggcgaaaa tattagcagc cgtgaagtag
       61 aagatatttt attgcagcat cctaaaattc acgatgcctg tgtggttgca atgcccgatg
      121 aacgtttagg tgaacgatca tgcgcttatg tcgtgctgaa agcgccgcat cattcattat
      181 cgctggaaga ggtggtggct ttttttagcc gtaaacgggt cgcaaaatat aaatatcctg
      241 aacatatcgt ggtaatcgaa aaactaccgc gtactgcctc aggtaaaata caaaagtttt
      301 tgttaagaaa agatattatg cggcggttaa ctcaggatgt ctgtgaagag attgaataag
      361 tttcatcttc ggggatcaca taaccccggc gactgaacgc cgccggggat ttattttatt
      421 tcttcagttc agccaagctt aaccaggttt gcaccacggt gtccgggttc agagacaggc
      481 tatcgatccc ctcttccatc aaccatgcgg caaagtcttc gtggtcggac ggaccctgac
      541 cgcaaatccc gacatatttg ccctgtttct tcgcggcacg gatagccatc gacagcagtg
      601 ctttcaccgc atcgttgcgc tcatcgaaca attcagacac cacgccggag tcacggtcca
      661 gaccgagcgc cagctgcgtc atatcgtttg agccaattga gaagccgtcg aaatattcga
      721 ggaactgctc ggccagcaag gcgttggacg ggatttcaca catcatgatg attttcagcc
      781 cgttctcacc acgcttcagc ccctgacgcg ccagttcctc aaccaccgct ttcgcctgat
      841 ctacggtacg cacgaacggg atcatgatct caacgttggt cagccccatg tcgttgcgca
      901 cacgtttcac tgcttcgcac tccagcgcga agcagtcgcg gaagctgtcg gaaacatagc
      961 gtcccgcgcc acggaagccg agcatcgggt tctcttcatc tggctcgtaa cgctcaccac
     1021 cgaccaggtt ggcatattcg ttcgatttaa aatcagagag acggacaatg acgcgcttcg
     1081 gataaaacgc ggcacccagc gtcgcgatcc cttcagtcag acgaccaacg taaaattcac
     1141 gcggagaatc aaaacctttc atcatctcgc ggatttcatt ttgcaactgc ggttcctgat
     1201 cgtcaaactc aagcagtgcg cgtgggtgga cgccaatcat acggttgatg ataaattcca
     1261 gacgcgcaag gcccacgcct tcgttcggca ggcaggcgaa gtcgaaagca cggtccgggt
     1321 taccgacgtt catcatcact ttcaacggca gatccggcat catttctacg ctggagcttt
     1381 tcacgctaaa ttccagcaac tccgcataga cgtaaccggt atcaccttcg gcacaagaaa
     1441 cagtgacgtt ctcaccgtct ttcatccgtt ctgttgcatc accacagccc actaccgccg
     1501 gaatgcccag ttcacgagcg atgatcgccg cgtgacaggt acgaccgcca cggttggtga
     1561 cgatggcaga tgctttcttc atgatcggtt cccagtccgg gtcggtcatg tcagtgacca
     1621 gcacgtcacc aggttcgatg cggttcattt cgctgatctc atggatgact ttcaccggac
     1681 ccgcgccgat gcgatgaccg atagcacggc cttcggcgat aatcttaccc tgtgaatgca
     1741 gcgtataacg ctccatgacc tgaccgcgtg agcgcacggt ttccggacgc gcctgcacaa
     1801 tgaacagttt gccagtgtgg ccatctttcg cccactcaat atccatcggg cgaccgtagt
     1861 gtttctcaat ttgtacggcc tgttttgcca gttcctgcac ttcttcgttg gtcagcgaga
     1921 agatgtcacg ctgttcctgc ggtacgtctt cgattttaac ctgcttgccg tgctcctggg
     1981 tcggcgcgta aaccatgcgg atttttttcg accccatggt gcgacgcacg atagccgggc
     2041 gattcgccgc cagtgtcggt ttatggacgt aaaactcatc cgggttaacc gcaccctgca
     2101 cgaccatttc cccaaggccc catgcggaag tgataaacac cacctggtca aagccggatt
     2161 cggtatcaat ggagaacatc acgccagaag atgcgaggtc ggagcgcacc atccgctgaa
     2221 caccggcaga gagcgccacg ccacggtgat cgtaaccctg gtgcacacga taagagatgg
     2281 cgcgatcgtt aaacagagaa gcaaatacat gcttcactgc cacgagaacg gcgtcaaaac
     2341 cctgaacgtt gaggaaggtt tcctgctgac cggcaaaaga agcgtccggc atatcttctg
     2401 cggtggcgga ggagcgcacc gcaaaagagg cgttttcgtc atcggcggaa agctgtgcat
     2461 aggcttcgcg gatggcgttt tccagctcag gctggaaggg agtgtcgata atccactggc
     2521 ggatttgcgc gcccgctttc gcaagctgag taacatcgtc aatatccgtt ttatccagca
     2581 gttcataaat gcgctggttt acgccgcttt ggtccagaaa ctggttaaac gcgtcggcgg
     2641 ttgtggcgaa accattcgga acggaaacac ccattccgga aaggttagta atcatttcac
     2701 ccagggaggc atttttgccc ccaaccctgt ctacatcatt catgccgagt tggttatacc
     2761 aaagcaccag cggtgacgag ccattgttgg acatcgaaca atccttttgt gataaatgaa
     2821 cggtttgaga aacacatttc tgcgcattta tctttgcata tttaaccgga tgaaaaaaac
     2881 ggtgaatcgt tcaagcaaat atattttttt actttttaag actgatccca gcgttgcgca
     2941 aatctgctct cttcgacaat ttccacataa accatcggta taaacgaaac cataaaaatg
     3001 aaatgctgtt ttcataaaaa ataaaattga aggttcattt tataaaccag acataacgtc
     3061 ctacgcttct gtgcgttttt aatttatgct ttcatagaat tatgtctgca tcacgggaag
     3121 aacaaaatgg ataatgctgt tgatcgccac gttttttata tttctgatgg tacggcaata
     3181 actgcggagg tattaggaca cgcagtaatg tcacaatttc ccgtcactat cagcagcatt
     3241 acgctgccgt ttgtcgaaaa tgagagccgt gcacgggcag tgaaggatca gattgacgca
     3301 atttatcacc agacaggtgt gcgcccgctg gtcttctact ccatcgtgtt gccggagatt
     3361 cgcgccatca tcttgcaaag tgaaggcttt tgccaggata tcgttcaggc gctggttgcc
     3421 ccgctacaac aagagatgaa actggatcca acgccgattg ctcatcgtac ccatggcctt
     3481 aaccctaata atctcaataa atatgatgcg cgcattgcgg cgattgatta caccctcgcc
     3541 cacgatgacg gcatttcgtt gcgcaatctg gatcaggctc aggtgatcct gctcggtgtt
     3601 tctcgctgtg gtaaaacccc caccagtctt tatctggcaa tgcaatttgg tatccgcgcg
     3661 gcaaactacc cctttattgc cgacgatatg gataatctgg tgctacccgc gtcgctcaaa
     3721 ccgcttcagc ataaattgtt cggcctgact atcgacccgg aacgtctggc ggcgattcgc
     3781 gaggaacgtc gggagaacag tcgctatgcc tcgcttcgtc agtgcaggat ggaagtcgcg
     3841 gaagtggaag cgttgtaccg taaaaatcag atcccgtgga ttaacagtac caattattcg
     3901 gtagaagaga ttgccaccaa gatcctcgat atcatgggcc ttagtcgccg aatgtactag
     3961 agaactagtg cattagctta tttttttgtt atgatgctaa ccacccggcg aggtgtgaca
     4021 cacctcgcac ttgaaatcag cagcgattgg tttatcgtga tgcgcatcac ttcccggcag
     4081 tcctgccgta gaagcaacaa atttctgaga cttgtaatga acagaactga cgaactccgt
     4141 actgcgcgta ttgagagcct ggtaacgccc gccgaactcg cgctacggta tcccgtaacg
     4201 cctggcgccg ccacccatgt caccgactcc cgccgcagaa ttgaaaaaat actcaatggt
     4261 gaagataagc gactgttggt cattattggc ccctgctcga tccacgatct caccgctgca
     4321 atggagtacg ccccccgtct gcagtcgctg cgcaaccagt accagtcacg gctggaaatc
     4381 gtaatgcgca cctattttga aaaaccacga actgttgtcg gctggaaagg actaatctcc
     4441 gatccagatt taaacggcag ctatcgggta aatcacggtc tggagctggc gcgcaaatta
     4501 cttttacagg taaatgagct gggcgtccca accgcgactg agttcctcga tatggtgacc
     4561 ggtcagttta ttgctgattt aatcagttgg ggcgcgattg gcgcacgtac taccgaaagt
     4621 cagatccacc gtgaaatggc ttcggcactc tcctgtccgg taggttttaa aaatggtacc
     4681 gatggcaata cgcggattgc tgtggatgct atccgcgcag cccgcgccag ccatatgttc
     4741 ctctcgccag acaaaaatgg tcagatgacc atctatcaga ccagcggcaa cccgtatggc
     4801 cacattatta tgcgtggcgg caaaaaaccg aattatcatg ccgatgatat cgccgcagcc
     4861 tgcgatacgc tgcacgagtt tgatttacct gaacatctgg tggtggattt cagccacggt
     4921 aactgccaga agcagcaccg tcgccagtta gaagtttgtg aggatatttg tcagcaaatc
     4981 cgcaatggct ctacggcgat tgctggaatt atggcggaaa gtttcctgct cgaaggaacg
     5041 caaaaaatcg tcggcagtca gccgctcact tacggtcaat ccattaccga cccgtgtctg
     5101 ggctgggaag ataccgaacg cctggtcgaa aaactcgcct ctgcggtaga tacccgcttc
     5161 tgaatgcgtg cccattcctg acggaatggg catttctgcg caacttgttg tcttctcaac
     5221 aaattactgc ttgctctggc ctgccataat attgataata agaatcattg ttatatcaat
     5281 tattattaat ctttatgcgt tatacggata gcagaaaact cacgcctgaa acggacgcca
     5341 atcacaaggc cgcttccccg cagcccatcc ggcgaattac cagccagaca ctgttaggtc
     5401 cggatggcaa actgattatc gatcatgacg ggcaagaata tctgctccgt aaaactcagg
     5461 ctggcaagct gctgttaact aagtagcctt taactcgagc agctgacttc cagccgttta
     5521 ccccagtcag gtggacgact gacatagtca tcatctctat cgctgaaagg atttcgcaac
     5581 gccccatgca ggcggtgcaa ttccgtcata tcaccctttt ctgccgcttc aatcgcccgt
     5641 tgcgccaacc agttgcgcaa taccagagcg gggttaacgc tttgcatcaa ctgctgacgc
     5701 tcactatcgc taacctcgtc ttgttgcaaa cgcccccgat aacgggcaaa ccagccatca
     5761 aatgccgcac gatcaataaa ctcatcacgt agcggtgacg ccgtgctgtg ctgctcggtc
     5821 agactcagca tgcggaatgt gcgggtataa tcgctgcgct ctcgcgccat cagactgaat
     5881 aattcattca gtagcgcgtt atcctctttt tgctccgtga tgaagcccag tttctgccgc
     5941 atccgttgtc cataatgcgt caacaaaacc tgctgatagc tgtccagggc ctcattcagg
     6001 gcatctacgg caacaaatgg tgacaatgtc tgcgccagac gctgtaaatt ccacaacgcg
     6061 acagcaggtt gattatcaaa actataacgc ccttgatgat ccgagtgatt acaaataaaa
     6121 ccgggttcgt aatcatcaag aaaaccaaac ggcccgtaat caagcgtcag tcccagcagc
     6181 gacatgttgt cggtattcat caccccatgt gcaaagccga ccgtctgcca ttgggcaatt
     6241 aacgacgcgg tacgtgcgac aacatcgcta aaccagagac ggtatttgtc ctcatcatct
     6301 tcaagatgtg accagtaatg acgaatagca aagtcagcta actgacgaac cttttccgac
     6361 tcgcggcggt agtaaaaatg ttcgaaatga ccaaagcgca gatgacttgg tgccacacgc
     6421 atcagcatcg cgcctggctc cgccgtttcc cgatacactg gcgaatcgct ggtgacgata
     6481 cttaacgcgc gggtcgtcgg aatgcccaga taatgcatcg cctcactggc gagactttct
     6541 cgtatcgtcg aacgtaaaac cgcccgtcca tcacccattc gcgaataagg cgtcaggcca
     6601 gcacctttca gatgccagtc cattgtagtg ccatcagcaa gcagttgttc gccgagtaaa
     6661 atgccgcgcc catcacccag ttggcccgcc cagacgccga actgatgacc actgtaaacc
     6721 tgcgccagtg gtgacatacc aggcagtagg gcttcgccgc cccagacacc tgcgccattt
     6781 ttaaacagcg acgatggaat actcagcgtg ttagccagtt cggtattatg ccaaatcagc
     6841 cgggcattat ttaaaggcgt aggggaaagt gctgtatagg tttctggcaa ttcatcgcgc
     6901 cagcgggtaa caaaagacag ggtcatagat cctcctgttt gatagtgtag acggttactc
     6961 tcgtcttaaa caccagcaaa cagaagggtt atcgctgaac caacgtcgtt atctgattta
     7021 tgggaacggc aggccacagg catccctgga gagcatggaa atcgaagggg gtaatctgtg
     7081 ccaatatttc tgccgtatca atgcctcccg caatgatgca attgcaacaa ggggaaattt
     7141 gcgcctggat cgcacgaata aacggttcga aggagcggtg cgtgatttgt tgctgaataa
     7201 aacttttatc cagcatgacg cgggtgaaaa gaccatcaaa aaccgctttc attgtgctat
     7261 tgcctgctcc caaattacct aacacaaggg gatacacttg cgataaggat aaaagaccgc
     7321 gattatcttt tccttcattg agatgcgggt aattctcatt aatcaacagt tcaataaacg
     7381 gatattttaa tagttcgcct gcgtaattat ccctctctaa taataatgta gccacttgtg
     7441 gcgttaaatt aagccaggca aagagtttat gttgaataaa aaaatgttgg catgatttta
     7501 gtaattccaa ctgctctgaa aataattgcc aatgttgctc ctcggtaagt tgcgctataa
     7561 cccgactcgt cgggatacgc accgtgccat cttcactgga gaaatgggta attaactcaa
     7621 cgccgacaag gacttgctgg ttatctctta tcggcaggaa atagcaatca gaatgataaa
     7681 gattctccaa aaaaatcttc atggtaaccg tccctctcga aggatgtttt tcagtatccg
     7741 gctaaaaggg atgaggccat aagacataac aattacagaa ggagtaactt tcatttgttc
     7801 catgttaacc actttttcag gggtcctttt tttagattat cctgattata aacgaataat
     7861 ccatttacgg aatttttgtc tgcaaaatac tactgtattc aggagtaaat ggacggcgat
     7921 gtatcatcgc cgtccatttt tcgac
//
LOCUS       DAZPDU010000191         7945 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZPDU010000191 DAZPDU010000000
VERSION     DAZPDU010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7945
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.193"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..496
                     /gene="radC"
                     /locus_tag="ACHURS_003809"
     CDS             <1..496
                     /gene="radC"
                     /locus_tag="ACHURS_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFR1239434.1"
                     /translation="LTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            713..949
                     /gene="rpmB"
                     /locus_tag="ACHURS_003810"
     CDS             713..949
                     /gene="rpmB"
                     /locus_tag="ACHURS_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFR1239435.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            970..1137
                     /gene="rpmG"
                     /locus_tag="ACHURS_003811"
     CDS             970..1137
                     /gene="rpmG"
                     /locus_tag="ACHURS_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFR1239436.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            1235..2044
                     /gene="mutM"
                     /locus_tag="ACHURS_003812"
     CDS             1235..2044
                     /gene="mutM"
                     /locus_tag="ACHURS_003812"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFR1239437.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2083..2562)
                     /gene="coaD"
                     /locus_tag="ACHURS_003813"
     CDS             complement(2083..2562)
                     /gene="coaD"
                     /locus_tag="ACHURS_003813"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFR1239438.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALTQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALVAKLA"
     gene            complement(2570..3847)
                     /gene="waaA"
                     /locus_tag="ACHURS_003814"
     CDS             complement(2570..3847)
                     /gene="waaA"
                     /locus_tag="ACHURS_003814"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFR1239439.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            4260..5318
                     /gene="rfaQ"
                     /locus_tag="ACHURS_003815"
     CDS             4260..5318
                     /gene="rfaQ"
                     /locus_tag="ACHURS_003815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFR1239440.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTTPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            5315..6439
                     /locus_tag="ACHURS_003816"
     CDS             5315..6439
                     /locus_tag="ACHURS_003816"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFR1239441.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYHLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            6432..7229
                     /gene="rfaP"
                     /locus_tag="ACHURS_003817"
     CDS             6432..7229
                     /gene="rfaP"
                     /locus_tag="ACHURS_003817"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFR1239442.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLSRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAVGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFVAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            7245..>7945
                     /locus_tag="ACHURS_003818"
     CDS             7245..>7945
                     /locus_tag="ACHURS_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFR1239443.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAVVSRLTY
                     MDQDILNLILSGKVK"
BASE COUNT         2021 a         1836 c         2032 g         2056 t
ORIGIN      
        1 gttaacctct gaatatgaac aatttagtgg cgttcatgga attggcgtag cgaaatttgc
       61 ccagttaaag gggattgctg aactggcgcg gcgttactac aacgtgcgga tgcgtgaaga
      121 aagcccttta ctcagcccgg agatgacgcg ggaattttta caaagccagc tcacgggtga
      181 ggagcgggag atctttatgg tgatctttct cgactcacaa caccgggtta taacgcatag
      241 ccgtcttttt tccggcacgc taaaccatgt tgaagtccat cctcgggaaa ttatccggga
      301 agcgataaaa ataaacgcct cggcgctgat ccttgcacat aatcaccctt cgggttgtgc
      361 tgaacccagt aaagcggata aactcattac tgaacggata ataaagagtt gtcagttcat
      421 ggatttacgc gtgctcgacc atatcgtgat tgggcgtgga gagtatgttt cttttgccga
      481 acgcggctgg atttaacccg ctatgcgcga tccttcggga tctttgtctg ttcgggactt
      541 gagcacatcg ctgagtcagc gtatactacg ccacctttga gaatctcggg tttggcattt
      601 gggcctggca atcgagagtt cacatagaac tgcgatgacc gggctgtaaa gcctgacgag
      661 gcgccaatac cccatacgaa gctcgagcta atttgatttt tggagaatag acatgtcccg
      721 agtctgccaa gttactggca agcgtccggt gaccggtaac aaccgttccc acgcactgaa
      781 cgcgactaaa cgccgtttcc tgccgaacct gcactctcac cgtttctggg ttgagagcga
      841 aaagcgtttt gtcaccctgc gcgtatctgc taaaggtatg cgtgtaatcg ataaaaaagg
      901 catcgataca gttctggctg aactgcgtgc ccgtggcgaa aagtactaag tacttagagg
      961 aaataaatca tggctaaagg tattcgtgag aaaatcaagc tggtttcttc tgctggtact
     1021 ggtcacttct ataccactac gaagaacaaa cgtactaagc cggaaaaact ggaactgaaa
     1081 aaattcgatc cagttgttcg ccagcacgtg atctacaaag aagcgaaaat caaataattc
     1141 tcgctttgat gtaacaaaaa acctcgctcc ggcggggttt tttgttatct gcttgccccc
     1201 atattgattg catctgttca ttcctggaga tgctatgcct gaattacccg aagttgaaac
     1261 cagccgtcgc ggcatagaac cgcatctcgt tggtgcaacc attcttcatg cggtggtgcg
     1321 caacggacgc ttgcgctggc cggtttcaga agagatctac cgtttaagcg accaaccagt
     1381 gcttagcgtg cagcggcggg ctaaatatct gctgctggag ctgcctgagg gctggattat
     1441 cattcatttg gggatgtctg gcagcctgcg catccttcca gaagaacttc cccctgaaaa
     1501 gcatgaccat gtggatttgg tgatgagcaa cggcaaagtg ctgcgctaca ccgatccgcg
     1561 ccgctttggt gcctggctgt ggaccaaaga gctggaaggg cacaacgtgc tggctcatct
     1621 tggaccggag ccgcttagcg acgatttcaa tggtgagtat ctgcatcaga agtgcgcgaa
     1681 gaaaaaaacg gcgattaaac cgtggctgat ggataacaag ctggtggtag gggtagggaa
     1741 tatctatgcc agcgaatcac tgtttgcggc ggggatccat ccggatcggc tggcgtcatc
     1801 actgtcgctg gcagagtgtg aattgttagc tcgggtgatt aaagcggtgt tgctgcgttc
     1861 gattgagcag ggtggtacaa cgctgaaaga ttttctgcaa agtgatggta aaccgggcta
     1921 tttcgcgcag gaattgcagg tttacgggcg aaaaggtgag ccgtgtcggg tgtgcggtac
     1981 gccgattgtg gcgactaaac atgcgcagcg ggcaacgttt tattgtcggc agtgccagaa
     2041 gtaattcata cgcgccggat ggcataccat ccggcataaa cgctacgcta acttcgccac
     2101 cagcgcctga tggacattct ccggcaggaa atgggtgaca tcgccctgat ggcgcgccac
     2161 ctctttcacc aacgatgaag agataaacga ccactctttc gacggcatca gaaacacact
     2221 ttccagttcc ggcattaagt ggcgattcat atgcgccagc tgcatttcat attcaaaatc
     2281 cgccaccgca cgcaggccac gaatcagcac cgtagcgtgt tgattacggg cgaagttcgc
     2341 catcaaatca ctaaacccaa ccacttccac gttccccaga tgcgcggttg cctgctgtgt
     2401 cagcgccaca cgctcttcca gggtaaacat cggtttttta ctggggctgg cggcaatcgc
     2461 cagaataacg tgatcgaaca tttgcgtggc gcgcgtcacg atatcgatat gaccattggt
     2521 aatgggatcg aaagtacccg gataaatcgc ccgtttttgc ataacaacct caatgcgttt
     2581 tcggtggcag gtaaggttcc agcagttgaa gcagacgctg tagcgcgccc tggttttgat
     2641 acagtacttc aacggcatga cggccataga aactacggta atcggcgtcg gtgagtaaag
     2701 aggaaacctc ttttgcaagc gtagtggcat cggtaacggt aatcagcccg cttgcctgct
     2761 ccagccgcgc gcaaatgtct ttgaaattaa aagtatgcgg ccccatcaat accggaatag
     2821 cgtgtgcggc agcttccaga ggattatgcc ctccacgttc aaccagtgaa ccgccaacaa
     2881 aggcgagatc ggcaatgccg tacagtaaca tcaactcgcc catcgtatcg ccaaccacaa
     2941 cctgcgtgct ggtggagggg acttcccctg aagagcgtgt gatatagctt agtccagcct
     3001 ggcggacaag gttaatcgca tccgggaagc gttctggatg acggggtacc aggatgagca
     3061 ataaattcgg gaattgctgt aataatgcct gatgtgcggc gatcaccaca ctctcttcac
     3121 cttcgtgagt gctggtggca atccataccg ggcggtgtgg tgcccactgg cggcgcagcg
     3181 tcacggcttt agcagccaac tgcggcgtta cagaaatatc gaatttcagg ctaccggtaa
     3241 cggttacctg gttatttttt gcgcccagcg ccacaaaacg tgcaccatct tcttcatttt
     3301 gcgcagcaat cagcgtaata cgacgcagta agcggcggac gaatttaccc agtttggcat
     3361 aacctgcggc cgagcgggca gagagtcgcg cgttagcgat caccagcgga attttacgtt
     3421 tatgtagcgc cgcaatcagg ttaggccata gttcggtttc cataatcaac accagtttgg
     3481 ggtcgacttt attcaggaaa cggttgagcg catcgggcag gtcatacggc agataaacgt
     3541 gctgaacatc cttcccgaaa gccgattgta cgcgctccga accggttggc gtcatggttg
     3601 taacggtaat cggtaaatca ggataacgat ggcgcagcgc gcgcaccaac gggatcgccg
     3661 ccagagtttc accgacggag acggagtgca gcataatgcc gcctggtttt agcggatggc
     3721 ggtaaaaacc gtaacgttca ccccagcgtt ttcgataggc cggagcctta cgtccgcgca
     3781 cccagagccg tatccagatc agcggctgaa taaggtagag aagggcggtg taaagcaatt
     3841 cgagcatagt aaatagctga cttatggatg tgctggggat tctatgtatt tagctgtggc
     3901 tttaccatta cttttcccgt ttttgactta aatagcaatt tacccgccaa ttcttgctga
     3961 acatctatac tttttttgta tttttatgcg attcattgat ttctgctgta cgtgcaggaa
     4021 ggaatcgcag agcaacagtg aaagaatatg gttgctaatg attttatttg attgatttct
     4081 ttattgaaaa tcaacaatgt gcttaaaaag cattcgggtc ggattgccgc gaaagaagtg
     4141 atacactagc gcgtcaatat cagtgcggta tcagtattca ggtagctgtt gagcctgggg
     4201 cggtagcgtg ctttttctgc ttcaccttaa tcggataatc tcaacaaaaa gagttacttg
     4261 tggataagcc atttcgaaga attttgctca ttaagatgcg ttttcatggg gatatgttat
     4321 taactactcc cgtcattagt tcgctgaaaa aaaattaccc tgacgcaaaa atcgatgtgc
     4381 tgctttatca ggacaccacc ccgatcctgt ctgaaaatcc agagattaac gcgctctacg
     4441 gcataaaaaa taaaaaagca aaagcctcag aaaaaattgc caactttttt catctcatca
     4501 aggtattacg tgccaataag tatgacctta tcgtcaatct taccgatcaa tggatggttg
     4561 ctatactggt tcgcttatta aatgcccgtg tgaaaatttc ccaggattat catcatcggc
     4621 agtctgcttt ttggcgtaaa agtttcaccc atttagtgcc gttgcagggt ggaaatgtgg
     4681 tggaaagtaa cttatccgtg ctgacaccat tgggacttga ttcgttggtg aagcagacaa
     4741 ccatgagtta cccgcctgca agctggaaac gtatgcgtcg cgaacttgat cacgctggtg
     4801 ttggacaaaa ttatgtggtt atccaaccta cggcgcggca aatcttcaaa tgctgggaca
     4861 acgccaagtt ttccgctgtg attgatgcct tacatgctcg tggttatgaa gtcgttctga
     4921 cgtccggccc agataaagac gatctggcct gcgtcaatga aattgcgcag ggatgccaga
     4981 cgccaccagt aacggcgctg gctggaaagg tgaccttccc ggaacttggt gcgttaatcg
     5041 atcatgcgca gctgtttatt ggcgttgatt ccgcaccggc gcatattgcc gctgcagtta
     5101 atacgccgct gatatcgctg tttggtgcga cagaccatat tttctggcgt ccttggtcaa
     5161 ataacatgat tcaattctgg gcgggagatt accgggaaat gccaacgcgc gatcagcgtg
     5221 accgaaatga gatgtatctt tcggttattc cggcggcaga tgtcattgct gctgtcgata
     5281 aattactgcc ctcctccacg acaggtacgt cgttatgatc gttgcttttt gtttatataa
     5341 atattttccc tttggcggtt tgcagcgcga ttttatgcgt attgctcaga cagtcgccgc
     5401 ccgaggtcat catgttcggg tttataccca gtcgtgggaa ggcgaatgcc ctgatgtatt
     5461 tgaactgatc aaagtgccgg ttaaatcgca taccaatcac gggcgcaatg cggagtattt
     5521 tgcctgggtg caaaaacatt tacgcgaaca tcccgtcgat aaagtcgttg gattcaacaa
     5581 aatgccgggg ctggacgttt attatgccgc tgatgtttgt tatgccgaga aagtcgcgca
     5641 ggaaaaaggc tttttctatc acctgacgtc acgttatcgc cattatgccg cctttgagcg
     5701 ggcaaccttc gaacagggca agccgacaca gctgctgatg ctgacagata agcaaatcgc
     5761 cgatttccag aaacattatc agactgaagc ggagcgtttt catattctgc caccggggat
     5821 ttatcctgat cgtaaatata gccagcagcc agctaatagc cgtgaaatct tccgtaagaa
     5881 gaatggaata accgaacaac aatatttatt gttgcaggtc ggttcagact tcacgcgtaa
     5941 aggtgtcgat cgttccattg aagcacttgc ttcgttaccg gattcgctgc gccacaacac
     6001 attgctatat gttgttggac aggataagcc gcgaaaattt gaggcgctgg cagaaaaacg
     6061 cggcgtgcgc agtaatgttc acttcttctc ggggcgcaac gatgtctcgg aattaatggc
     6121 ggcggcggat ttattactgc atcctgccta ccaggaagca gcgggaattg tgctactgga
     6181 agcgattact gcaggattac cggtactaac aacagccgtt tgtggctatg cgcattatat
     6241 tgtcgacgcc aattgcggcg aggctattgc tgagccattc cgccaggaaa cattgaatga
     6301 gattttacgc aaagcgttaa cgcaatcttc attgcgccag gcctgggcgg aaaatgcgcg
     6361 acattatgct gatacacaag atttatacag tctgccagag aaagcggcgg atatcataac
     6421 gggtggtctg gatggttgaa cttaaagagc cgtttgccac gttatggcgc ggtaaagatc
     6481 cttttgagga agttaaaacc ttgcagggtg aggtttttcg tgaactggaa actcgccgca
     6541 ctctgcgatt tgaaatggcg ggcaaaagct attttctcaa atggcatcgc ggcacgaccc
     6601 tgaaagagat aatcaaaaat ttactctcat tgcggatgcc agtattaggc gcagaccgcg
     6661 aatggaatgc gattcatcga ctgcgggatg tcggcgttga tactatgtat ggggtggcat
     6721 ttggcgaaaa aggtattaat ccgctgagca gaacctcgtt tattattacc gaagatctga
     6781 ccccaaccat cagtctggaa gattactgtg ctgactgggc gaccaaccca ccagatgttc
     6841 gcgtaaagcg tatgcttatt aagcgtgtcg cgacgatggt gcgcgatatg catgctgtgg
     6901 gcattaacca ccgtgactgt tatatctgtc atttcctgct gcacttgcct ttttccggta
     6961 aggaagagga gttaaaaatt tcggtaattg acctgcaccg ggcgcagctt cgcacgcgcg
     7021 ttccacgtcg ttggcgcgat aaagatctta ttgggcttta tttttcttcg atgaatatcg
     7081 gcctgactca gcgggatatc tggcggttta tgaaagtgta ttttgtcgcc ccgcttaaag
     7141 acattctcaa gcaggaacaa ggactgctgt cgcaagcaga agcaaaagcc acaaaaatca
     7201 gggaaagaac gattcgaaaa tcgttgtaat ttccggagag aaatatgagt gcccactatt
     7261 ttaatccaca agagatgatc aataagacaa tcatcttcga tgaaaggcca gcggcgtcag
     7321 tggcatcatc attccatgtt gcttatggca ttgataaaaa ctttcttttt ggttgtggtg
     7381 tttcaatcac gtcagttttg ttacataaca acgacgtgag ttttgttttc cacgttttta
     7441 ttgatgatat ccctgaagcc gatatccagc gtttagccca attggcgaaa agctatcgta
     7501 cctgtatcca gatccatctg gtaaattgtg aacggcttaa ggcattaccg acgaccaaaa
     7561 attggtctat tgccatgtat ttccgttttg taattgcaga ttactttatt gatcaacaag
     7621 ataagatcct ttacctggat gctgatatcg cctgtcaggg aaacttaaag ccgctgataa
     7681 caatggatct tgccaataac gttgctgctg ttgttactga acgcgatgct aactggtggt
     7741 cgttacgggg tcaaagtctg cagtgtaatg aacttgaaaa gggttacttt aattcaggtg
     7801 tcctgttaat taatacacta gcgtgggcgc aggagtccgt ttctgctaaa gcgatgtcga
     7861 tgcttgctga taaagccgtc gtttcccgtt taacctatat ggatcaagat atacttaatc
     7921 ttatcctgtc agggaaagtt aaatt
//
LOCUS       DAZPDU010000192         7923 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZPDU010000192 DAZPDU010000000
VERSION     DAZPDU010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7923
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.194"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            177..905
                     /gene="gadW"
                     /locus_tag="ACHURS_003819"
     CDS             177..905
                     /gene="gadW"
                     /locus_tag="ACHURS_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFR1239444.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(1268..4381)
                     /gene="mdtF"
                     /locus_tag="ACHURS_003820"
     CDS             complement(1268..4381)
                     /gene="mdtF"
                     /locus_tag="ACHURS_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFR1239445.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(4406..5515)
                     /gene="mdtE"
                     /locus_tag="ACHURS_003821"
     CDS             complement(4406..5515)
                     /gene="mdtE"
                     /locus_tag="ACHURS_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFR1239446.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            5623..5901
                     /locus_tag="ACHURS_003822"
     CDS             5623..5901
                     /locus_tag="ACHURS_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239447.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYLGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(5902..6429)
                     /gene="gadE"
                     /locus_tag="ACHURS_003823"
     CDS             complement(5902..6429)
                     /gene="gadE"
                     /locus_tag="ACHURS_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFR1239448.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(7228..7800)
                     /gene="hdeD"
                     /locus_tag="ACHURS_003824"
     CDS             complement(7228..7800)
                     /gene="hdeD"
                     /locus_tag="ACHURS_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFR1239449.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
BASE COUNT         2109 a         1888 c         1853 g         2073 t
ORIGIN      
        1 gaaaatatat tcgtgctgca tttacttatt atcaattaac tgttatgcaa aactactttg
       61 tggataaatt ttggtcctac caaatctggc agttttttcg ctaagaaaca gtctggcaac
      121 atttcattag tatactgaaa ttgaaataat cgcagtatga aatataaggg ataatcatga
      181 ctcatgtctg ctcggtgatc ctcattcgtc gttcattcga tatttatcat gaacagcata
      241 aaatatcgct gcataacgag agtatcgtgc tgctggagaa aaatttggca gacgattttg
      301 cgttttgttc accggatacg cgacgactgg atatcgatga gctgacagtt tgccattact
      361 tacaaaatat tcgtcagcta ccacgcaatt tagggttaca tagcaaagac cgtctattaa
      421 ttaaccagtc accccccatg ccgctggtga cggcgatttt tgatagcttc aatgaatccg
      481 gggtaaattc accgatactg agcaatatgc tctacctttc ctgtttatcg atgttttctc
      541 ataagaaaga actgatcccc ttacttttca atagcatcag cactgtttca ggaaaagttg
      601 aacgccttat tagctttgat atcgccaaac gttggtatct gcgcgatatc gcggaaagaa
      661 tgtataccag cgagagtctc ataaaaaaaa agttgcagga tgaaaatacc tgtttcagta
      721 aaatattact cgcttccagg atgtcgatgg ccagacgatt actcgagtta cgtcaaattc
      781 ctctgcatac tattgcagaa aaatgtggct atagcagtac gtcgtacttt ataaacacat
      841 ttcgacaata ttatggtgta acgccacatc agtttgcgca acattcgcca ggtacctttt
      901 cctgacatat tttgcatttg aatattggtc aggatctcac acctgcttca tgtgaaactc
      961 ttccctgatg atttctgccg ggctaccggc tagttctctt tcgcagtttg ccaggcggta
     1021 taggtatttt caggaggaaa agactcggca tgtttgggat tattaagctg acaattcata
     1081 cgattacggg aatgtgggtc agtattgtgc tgttcaaatt gatgacgaat ggctggagtg
     1141 gtttctactt tcagtgctgt gtactaagcc tggtattcct gacggttagc tggttgttga
     1201 gtggtgaatg gttagcagga aagagtaagg ctgaaccttc acgttcaacc ttactctcat
     1261 ttacacgtta cgctttttta aagcgggcaa agagatgttc cactacaacg aaaaagaccg
     1321 gaacgaagta aattgccagc actgttgcgg caaacatccc gcccatcacg ccggtaccta
     1381 ccgcgttttg cgcgccagaa ccggcaccat gactgataac cagcggcagc acgccgagaa
     1441 taaaggcgag agaggtcatc aggattgggc gtaaacgcat ccgcgccgct tcgatgattg
     1501 cctctatcgg cgttttccct tctttctgca tcatctcaac ggcaaattcg acaatcagga
     1561 tggcgttctt ggcggaaagc ccgatggtgg tcagcaaacc aacctggaag tagacgtcat
     1621 tacttaagcc gcgcagatcg gtggccagta atgcgccaac gacgcctaac ggaacaacca
     1681 acatcaccga gaacggaatt gaccagctct catagagtgc ggcgagggcg aggaacacca
     1741 cgaccagtga aatggcatac agtgcaggag cctggtttga ggataacgct tcctgatacg
     1801 acagtccggt ccatgagtag ccgacgcctg ccggaagttt agcgaccagg tctgccataa
     1861 atttcatagc gtcaccggta cttttcccgg ccgccgcttc acctaaaatc tccattgacg
     1921 ggatgccgtt gtagcgttcc aatcgcggtg aaccataggt ccattcggta gacgagtagg
     1981 cagaaagcgg tgccatcgtg ccagaggcgt tgcgtacata ccattggttg atgttatccg
     2041 gcaacatacg gaacggcgtg cctgcctgga catacacttt tttcacccgc ccctggttga
     2101 ggaagtcgtt cacgtagctg ctgccgaagg cggtggaaat tgtctggttg atatcagaca
     2161 gcgccacacc cattgcctca gctttcgcgg cgttgacgtt cactttgaac atcggcgtat
     2221 cttccaggcc gttcgggcgt accccggtga cctggtcagg cgattgcgct gccagtgata
     2281 acagctcgtt tcgtgcctgg gttagttttt cgtgccccag gttaccgttg tccagcagtt
     2341 ccatatcaaa acctgacgcg gtacccagtt cagccaccgc gggtaagttg aacgggaaga
     2401 cgacggcttt attgatactg cttaacgcaa tcattgcccg ctgaatgatc gcggtaaccg
     2461 agttttcctc accgacacgt tcagaccacg gcttgagact gataaacgcc aggccgttgt
     2521 tttgcccctg gccgctgaag ccaaagccgc caacggtaaa caccgactga acattatctt
     2581 tctcttttgt aagataataa tccgtcacct gttgcagcac tttcgtggtg ttaaccatgg
     2641 tggcaccgga aggtaactgc gcggtggtca taaatacccc ctgatcctct tctggtaaga
     2701 aagaggtcgg tgtacgcagg aacagcaccg ccatcccggc gcaaatcagc aggtagacca
     2761 ccatgtagcg accggtacaa cgcaacagcg agcgggtgct atcggtatag tgctgagttg
     2821 atttttcaaa cagcgtgttg aagcgtgcga acagggcgtt aggtttgtga ccgccttccg
     2881 gcgcggcttt cagaatggtg gcgcacaggg caggggtcag gctcattgcc acaaatactg
     2941 aaagcagcat ggaggagatc agcgtgatgg agaactggcg gtagatctcc ccggttgcgc
     3001 cgctcataaa ggccatcggc ataaacactg cggaaagaac aacagcaata ccgaccagcg
     3061 cacgttggat ctgccccatc gatttatgcg tcgcttcctt cggtggtagc ttatcttccg
     3121 caatgacacg ctcgacgttc tccaccacca cgatggcgtc atccaccagt aaccctatcg
     3181 ccagcaccat cccgaacata gttaacgtgt tgatggtgaa accgaccgcc gacaagatcg
     3241 caaacgtccc gagaataacc accggtacgg caatcgtcgg gatgattgtg gcacggaaat
     3301 tctgcaaaaa cagatacatg accaggaaga cgaggatgat agcctcaacc agtgttttaa
     3361 aaacttcctg aatagaaatt tcgataaacg gcgtggtgtc gtaaggataa accgtcttca
     3421 gacttgcggg gaaataggct gataagcggt tcagctcctc tttgactgcc cgcgaggtat
     3481 ccagggcgtt tgctccggta gccagtttga tggcgatccc ggcagcaggt ttgccgttat
     3541 agcgcgccac ggtggaataa tcttccgctc caagttcgac gcgggcgaca tcacgcagca
     3601 gcacttgcga accatcttgc tgaactttca acaggatttt gccaaattct tccggcgttt
     3661 gcagacgcgt ctgcacaatg atcgaggcgt ttagttgctg gtctgccgcc tgtggcatgc
     3721 cacccagttg accaccggaa atctggttgt tttgcacctt aatctgggaa ataacatcgg
     3781 aaggtaccag attgtattta ttgagttttt gcgggtccag ccagatacgc atggcgtact
     3841 cggaaccaaa gagctgtacg ctaccaacgc ccgcggtacg gcttagcggg tctttgatat
     3901 tagacgctac atagtccgcg atatcgtact ggttgaggct gccgttatca gaaataaacg
     3961 ccgctaccat cagaatatta ctgctcgact tatcaacgct aatcccctgc tgctgcaccg
     4021 cttcaggtaa tgaaggcata gcgagttgca gtttattttg cacctgaacc tgtgcgatat
     4081 caggagatgt cccagtctcg aaggtcagag tgatagaggc attacccgcc gcatcactgg
     4141 ttgaagacat gtacatcagg ccatcaagcc cattcatatt ttgctcaatc acctgagtga
     4201 ccgagtcttc taccgtttgc gcatcggcac ctggataggt agcgctgacg gtaatggtcg
     4261 gtggcgcaat ctgcggatac tgcgcaaccg gtaagttcat gatcgccaga ccacctgcaa
     4321 gcatcataat aatggcaagt acccaggcaa aaaccgggcg atcaataaaa tagttagcca
     4381 tgaaagtccc cttaagcctg caacgttatt gtttcgattc ggtgctggcg ttttcctggc
     4441 tggaggaaat tgctcgtgct ttgatacccg gacgaatgcg ttgcaaaccg gaaacgatca
     4501 cccgatcgcc agcctgcaag ccagaggtga caacccactg gtcgccgatg gctttgctgg
     4561 cttcaatttc gcgtagctgc acaacatcgt ctttatccag aatgagcgcc gttgctttac
     4621 cctgggcgtt gtgagtgacg ccttcctgcg gcaccagtaa tacattctgg cggctacctt
     4681 catccactaa tgccgtgacg tacatgccag gcagcaagtc accatttggg ttggggaaaa
     4741 tcgcccgtaa cgtcacggag cccgtggttt catccaccgt cgggtcggag aatttcagtg
     4801 tgccggtctg gctgtagcgt ttaccatttt ccagattgag ctgtactggc gtactgccct
     4861 gaacctgttt gatttgccca ctggcgacct cttctttcat gcgcaagaaa tcctgcaccg
     4921 actgcgtgag atcgacataa atcgggtcca gacgttgtac ggtaaccagc gaatctgcct
     4981 gattagcggt aacgagtgcg ccgacggtca ccgacgattt cccgctgacg cccgtaatcg
     5041 gcgaggtgac attcgcgtat tgcagattga tcgtcgcctg ttcaacagcc gctttggcga
     5101 cggtgacatt ggcttctgct tcattcagct gggtgcgcgc ggtgtcgtaa tcctgacggc
     5161 taacgtagtt ggtcttcagc aacgatgcct ggcggttaaa ggtgatgcgg gcattgctgg
     5221 cggtagagag cgctttcgcc agcgagcctt tggcggagtt tagctcggcc tgtaaaggtg
     5281 caggatcaat ctgatacagc gaatcgccct ggttcacttt atcgccttcg ataaagttgc
     5341 gtttaatgat aataccgccc acctggggac gtatctcggc aacttcataa ggaacggttc
     5401 taccgggcaa ttcgctcaac acattgaccg aaccggggga gagtgtgacg acaccgacct
     5461 caggcgtcat ggcggcggcg ttttccgccg atttgtcatc gcaggcggtg agcatcgcgc
     5521 cgcagaataa caacggtatt aacagctttc ttcttctgtt cattttagtc cctgaaaatt
     5581 cttgagaaat aacggttctt taaattgcgc gcgaggagag atatgaggga tttacagacc
     5641 agcggcatag tggggttgtc agccagtaag gtgggataca ggcacagtga tcgacatggt
     5701 gaggtcaacg acaaaaaaaa tgttgccgtt ctgccaacag ttcctgccag cattcgggca
     5761 acgaagggtt ctactggtgg atacacatac ctggggaata aagtcgtttt agcctctcct
     5821 ttagtaatca ccgggggtaa tgaaatcagc atctgcatcc ctcgtcatgc cagccatcaa
     5881 tttcagttgc ttatgtcctg actaaaaata agatgtgata cccagggtga cgatgtcgct
     5941 catacgtctt agtttcagct tgtacataat gttgaatttc tgaatcttta atgtttgttg
     6001 cgaaatattg agcgtcgacg tgatattgct ttcattttta tttttcagaa tcagttgcat
     6061 aattttgaac tgattatgtg aaaaacataa ttgtgaattc ttatggggca agtgtttacc
     6121 ataagtaatc tcaagaagat ttttgatact ttctttgcgg ctaacaaaat aaataggcgc
     6181 tttagctttt agtttactga tgtgataagg tgccaaaaca ataattctct cggcatctaa
     6241 tttctccaga aatttaatcg cttcttcatc aaggatatga ttgtgatagg tatcaatgat
     6301 aaccttgaag ggtttattgc ctactttttt gatctctgac agggaattga tttttatcat
     6361 ttcgtgatta tctttcaact gccaaaagcc ctgtaaaaga aaagaatctt tcgtcatgag
     6421 aaaaatcata acttgctcct tagccgttat cgtttcgaac atgtcatcct tttcattatt
     6481 tacatccttg tccgaatcgt tgttcaatat agtaaacgcc tataagaact ttacccttcg
     6541 tgaactaata cgtctattaa cactaatcaa tacttcagat ttatccatca cgcactccat
     6601 atattgatcc taagtaagat tcctggttgt tatcagcttg taaatagtaa acaaaaagtt
     6661 aacgtttgcg gattactgcc caagaataag ttatcaataa ccagtttgtg atctctgaag
     6721 aatattacta aagttaaaat gtaatccgat ttaaatatcg agtctccttg tttcgactta
     6781 agctggcaat tggattgcca gctttctttt aacgcattga aattacctga gtaaataacg
     6841 cattcatgtc ttcaggtttt tatatagcgt ctggagacac ggatatttat gcaatgtaat
     6901 caacgccagt tgcaaaaaaa tgaacccgga ttacttattt aaccgcccat agcatcaacg
     6961 cttacggaac tctttgttca ctttcccaca gcacttttaa aacattaaac ctgactacgg
     7021 aaaatatcag ccatgataat gtttgaaatg gctaatttgc catggagtga aaaaaattag
     7081 atgaaattca gtaggttgaa ataatcacta gcaggtaatt atttcaatga tagtgcgcaa
     7141 ttgatctaca acactccgta gcggagagag tattaatcgg atcatagtca tatcaagtga
     7201 ctatgatccg ggtgacaacc gggataatta ttgctgctta acgaacaaac tggcgaagct
     7261 gaacaggctg gcggcgctaa atatcagttc aattcccacc agtgtggaaa ccagcgttac
     7321 agacaccatc ggcgttgcac caaggaatat ccaggcaatg acgatatcca gcacaccaat
     7381 aacgagctgt agccagctgc ctttcattga acgctgacga taccaactca tcaggcgaat
     7441 aacccctgca acacagaaca aaccggcaat aaatgccgca atggcaaaaa tgcccagctc
     7501 cggtgcgcgg atgaagaaat agccgatcaa taaataggcg actgcgacga ggaaaccgga
     7561 taataccggc cagaaattat gactgcggtt gctgaataac ccgacaataa gcgcaatgcc
     7621 cgagcagatt aataatgcac ccactactgt gcttaaaata tcgccagaga cgaacgggaa
     7681 actgatacac agcaacccga cgataaacag tagcacggca ataaactgga ttgctctgcg
     7741 atgtttttta agcatctcca gatcaaactt caaaattgtt gccttatcta tatataacat
     7801 agaaccaccc tataaaatta agaagaaaat cccctgctat caatctatgc caaaaacgcg
     7861 tctaagaatg tagtcgattt aataaaaatt tcctaattac agtatctgat gcatctgtaa
     7921 ctc
//
LOCUS       DAZPDU010000193         7820 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZPDU010000193 DAZPDU010000000
VERSION     DAZPDU010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7820
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.195"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..903)
                     /gene="nac"
                     /locus_tag="ACHURS_003825"
     CDS             complement(<1..903)
                     /gene="nac"
                     /locus_tag="ACHURS_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFR1239450.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAKMNLFLPSDYSAIRLRVDEAFSLRRFTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQW"
     gene            1230..1305
                     /locus_tag="ACHURS_003826"
     tRNA            1230..1305
                     /locus_tag="ACHURS_003826"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1263..1265,aa:Asn,seq:gtt)
     gene            complement(1361..2296)
                     /gene="ldtA"
                     /locus_tag="ACHURS_003827"
     CDS             complement(1361..2296)
                     /gene="ldtA"
                     /locus_tag="ACHURS_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR1239451.1"
                     /translation="MMRRVNIFCSFALLFASHNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFISGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(2358..3437)
                     /gene="cobT"
                     /locus_tag="ACHURS_003828"
     CDS             complement(2358..3437)
                     /gene="cobT"
                     /locus_tag="ACHURS_003828"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFR1239452.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGVPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHLAEKLLLDVICYT
                     RELAKNGVTLFGVGDLGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(3449..4192)
                     /gene="cobS"
                     /locus_tag="ACHURS_003829"
     CDS             complement(3449..4192)
                     /gene="cobS"
                     /locus_tag="ACHURS_003829"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFR1239453.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(4189..4734)
                     /gene="cobU"
                     /locus_tag="ACHURS_003830"
     CDS             complement(4189..4734)
                     /gene="cobU"
                     /locus_tag="ACHURS_003830"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFR1239454.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPAHWHTVERWQHLDELIHADINPHEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(4868..5305)
                     /locus_tag="ACHURS_003831"
     CDS             complement(4868..5305)
                     /locus_tag="ACHURS_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HFR1239455.1"
                     /translation="MKTIKPESIALFCLLSGDVDLAKHLAEILPITCFVGEDWQQDFQ
                     PLDGGPVQSLSDAYSDYSVLLLIAAESEISVPEWLAHSEGTTVILIAEHNARLLTGPA
                     GGAVALIHYLNEICHSRETAVNDAFCRTLTRGSLAIPQCHLLS"
     regulatory      complement(5523..5698)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(5787..6463)
                     /locus_tag="ACHURS_003832"
                     /pseudo
     CDS             complement(5787..6463)
                     /locus_tag="ACHURS_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460981.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
     gene            complement(6666..7475)
                     /gene="pduF"
                     /locus_tag="ACHURS_003833"
     CDS             complement(6666..7475)
                     /gene="pduF"
                     /locus_tag="ACHURS_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol diffusion facilitator PduF"
                     /protein_id="HFR1239456.1"
                     /translation="MNDSLKAQCVAEFLGTGLFLFFGIGCLCALKVAGASLGLWEICI
                     IWGLGISLAVYLTAGISGAHLNPAVTIALWLFASFPARKVLPFCLAQLAGAFGGAVLA
                     YSLYSSLFTDFESAHNMVRGSAESLQLASIFSTYPAAAINVWQAALVEVVITSMLMGL
                     IMALTDDGNGVPKGPLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKIFTWLAGWG
                     EIAMTGGRNIPYFIVPIIAPIIGACVGAAIYRYLIAKNLPVNSVTPKGISE"
BASE COUNT         2190 a         2077 c         1664 g         1889 t
ORIGIN      
        1 ccactgcctt ttttccatca ctggggaact aatcactgac attaacaact ctttcactgc
       61 ctgtgcctgt ggcgataagt tcgctctggc gggtaaattt aatgacaaag agagactcat
      121 ggaaggagtg gtaatgcgtg acatccaccc atttactgcg ccacataacg aacgcgcggc
      181 cgattcgggt aatactgcaa cgcccatgcc gctggcaatc gctgcggtaa gcgtggcaat
      241 agactcaatt tcaccaatga cttttgccgt gaatcgccgt aaggaaaaag cctcatcaac
      301 acgaagtcta atagcactgt aatcactggg gagaaagagg ttcattttcg caatagcatt
      361 cacatcaacg ctttgccccg ggcaatcttg agttcctacc agaaaaagat cttctttcag
      421 caaagcctga ctggatacac cagccacagg ggaatgctca taaatcaccg ccatatcgag
      481 ttggtgattt atcaattttt cgttaagcac tgcaccacta ttttcatgaa gatagataac
      541 gatctccgga aattcagcgc gaaccgcctg taataagggc atggtgatgg atgacgcagc
      601 ggttcctggt gcaaagccaa tcgagacttg ccccgataat gcctgaccaa cgttatgcac
      661 cgccagttgg gcctgttcac actgacgtaa aatggcccgc gcatgggtat agagaatttt
      721 tccggcgtct gttggcgtaa cgccccgctt tgtacggatc aaaagttgtt gatttaactc
      781 accttccagt gtggcaacct gctggctgag cgctggttgt gcgatatgca atacttcagc
      841 agcctgggtc aggctaccaa tatctacaat ttttacgaag tatttcaggc gtctgaagtt
      901 catgttgcct ccggttttta agaatcggcc caagtgccgc cattacttac aaccagattg
      961 caagatgctt gccagtttta ttttggcgtt gatgtacaag ctaaccaact gtcaaataag
     1021 agattatgat agattcgtca tttgctcctt taatcagctg tcgcgttccc ctgccctata
     1081 aaaggagggt atgcaccacg atggttcatt acccaataag attgaaagct caccactttg
     1141 ttgaaattga cagcaaacaa acaaaaaaat gcatttcacc ctttgacatc accatgcact
     1201 gccattaata tgcgccccgt tcacacgatt cctctgtagt tcagtcggta gaacggcgga
     1261 ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg agccaaattc aaaaaagcct
     1321 gctttctagc aggctttttg ctttctaatt accaacgctc ttaaaacatc tgtcttgaac
     1381 cagaactaat ttgcacaggc attcccgatc gacgttgcaa cgcagcattt gcgcgattta
     1441 catcaacttc ttgcccgctg ataaacgccc gcaaagatgg ggttaccggc aatggcactt
     1501 ttcggtcaga ctcatattct gcacgattgc gcgacaacgg ctcatgaact tccagccagt
     1561 tcgagccatc tggttcagtg gtgtatttta ctggctggtc gataatttgc acacgcgtcc
     1621 caacaggaac attatcaaac agatatttga tatcgtcatt gcgcagacga atacagccct
     1681 gacttacccg gagcccaata ccaaaattgg cattggtacc atggatggca tacaacctgc
     1741 caatataaat cgcgtacagc cccatgggat tatcggggcc cgcaggaaca aatgcgggca
     1801 aactctcccc tcgtttcgca tattcgcgcc gagtgttcgg cgttggcgtc caggttggag
     1861 cttcttgttt acgttcaacg gtagtcaccc agttacgcgg ggtttctcgc ccagcctggc
     1921 cgataccaat aggaaagact tccacagtat tactgtctgg tgggtagtaa taaagacgca
     1981 tctcagcgac gttaaccaca atccctttac gaacagtggc gggcaaaatc agttgctgcg
     2041 gaatggtgag ttgcgagcca gacttcggca aaaaaacatc agcgcccggg ttcgcttcca
     2101 gcatgttact taacccttgc ccgtattgtg cggcaaaagt ctccagcggc tgggtattgt
     2161 gatcaggaac agttacagta agcgactgcc ccactaaacg gctaccctct ggaggtaatg
     2221 gataagttac cgccaggcta ttatggctgg caaaaagcag agcaaatgag caaaaaatat
     2281 ttacacgacg catcatgtcc ctttcctaag tcgcgaaagc tatccgttaa gtatagcttt
     2341 tatcagactt ttcgttttta actgttcaaa tcagaagtcg tattccccgg tagaacaata
     2401 ttactggcag caagttcgcc catgttgtta tatatcgcac aggcagcttc gatgatgggc
     2461 atcgccagag ctgcgccact cccctcacct aaacgcatct ccatattgag ataaggctcc
     2521 agccccaaat gcgacagcgc aatacgcgcc cctttttctg ccgacaggtg agaaggaatg
     2581 agatacggtt tgattgcagg agacatctgg caggctgcga gcgcggcagc ataagaaaga
     2641 aatccatcca gcaacacggg taaaccgcag gaagcagcgc ctaacatcac gccagccatt
     2701 ccgaccaaat caaatccacc cacttttgcc aggacatcaa cgccatcctg cggatttggt
     2761 tgattcaacg taatcgcccg gcgcacaaca tcaattttat tagccagttt atctgtcggc
     2821 aggtttgcgc caatcccaac cacttcttca ggagcccggc cagtgattgt gctgactatt
     2881 gccgctgccg gtgtcgtgtt tgccatcccc aggtcaccta caccaaacag cgtaacaccg
     2941 ttttttgcca gctcccgcgt ataacatatg acgtccaaaa gcaacttttc tgccagatgg
     3001 cgactcattg ccggagctga agcaatattg ccactacctc gagcaacacg catgttgata
     3061 agcccgggga taggctcagc agtatcaata ccaacatcaa ttacgtagac gttagcgccc
     3121 gcttgtgctg ccagcacaca cacgccggtt gttccgcggg tcatattttc agcctgaatg
     3181 gctgtcactt cttttgggga aatagcgacc ccttcctccc agacgccgtg atcggcacac
     3241 atgaccagta ccgctttttt gcccacatgc ggaacgccat tcaaccccgg cattcctgcc
     3301 agttgtatgg caagcgcctc cagccttccc aggctaccaa caggtttgag taacccgtcg
     3361 atatgccgtt gtgcacgcga catagcggca ggatcaatag cagggatcgt attcagtaaa
     3421 tcggcaagtg tttgcatgtc acgtctcttt atagcagtgc cagcaggaag accagttcac
     3481 caagctctat agcagctccc agcgtatcgc cggtttgccc gcccagcgta cgtttgagaa
     3541 gctgaccgag gatgaaaatc gccaccatcg tcaccaccat agcagccaca ccatgcatac
     3601 ccggcaataa tacggcagca aaaatagcgg ctaagccgag tgtgacgcag gtttgtcgcc
     3661 catcaatttt gccaatgaac acattgccaa gtccttcttc ccgggcataa cgatgacgat
     3721 acatcaataa tgcggcagtc ccgcggctga ccgcacatgc tgccgccagc gatgcaagga
     3781 tcggttcgcc acgcagagcc aactcgctta ataccagaat ctttgccagt acaacaaaaa
     3841 tcaatgccag accgccgtgg gtgcctaaac ggctatcacg catgatttcc aacatgcgat
     3901 cgcggctacg tgcagaaaat acgccatcac aggtatcggc gaggccgtcc aggtggaatc
     3961 ccccggtcat cagcgccagc acaagtacgc taaacagtgc cgccagtggt acgccacacc
     4021 atgcctgcag caccatgaag accagcccgc taatcgcgcc aagcaataat ccaattaaag
     4081 gaaaagtaat aataccgcga gaataatgct cgaaatccag tccctgggac cagcgacgcg
     4141 gtacaggcag acgcgtaata aaagagagca tcgcccaaaa taatttactc atttgatttt
     4201 tactccaata cccgaaacca ccaaccatac ttcatttgcc gcagcggcca gctgctgatt
     4261 tacccgcccg gcaatatcac gaaaatgtcg tgccagacga ctctccggca caatccccat
     4321 tcccacttcg ttagtcacta atacaacctt tgcgggacaa cgttggcagg cagcaatcaa
     4381 cgactgaatc tcagcattaa tcgcctgttc catcgcctga taatcccatt catcagggtc
     4441 tttatcgccg ccataatcaa acaacagatt agtcaccatg gtggtaacgc attccagcaa
     4501 tacagcctca tgcgggttaa tgtctgcatg aattaattca tcaagatgtt gccagcgctc
     4561 cactgtgtgc cagtgcgccg ggcggccttg ccgatgatgt tctatccgtg cagccatctc
     4621 atcatcaagg atttgtgagg tggcgatata cagaacctgc gaagagtccc caataagcgc
     4681 ctctgcatgg cgactcttcc cgctccgtgc gccccccgtc accaaaatca tcataccgac
     4741 tcctgattca attgtatgat ggtattcatt gaacagaaca atggatcccc caccgttgct
     4801 gaccgcaaaa catttacgcg attaatactg cgcgtaatat aattaaaaat ccagcattct
     4861 caatccatta cgaaagaaga tggcattgcg gaatagccag acttcctcgt gttaacgtcc
     4921 gacaaaatgc gtcattgaca gcggtttctc tggaatgaca gatttcattc agataatgga
     4981 ttaatgctac agccccacca gccggaccag tcagcaaacg ggcattgtgt tctgcaatca
     5041 aaattaccgt cgttccctca gaatgagcga gccattctgg tacggatatc tcgctttcag
     5101 ctgctatcaa tagcagcact gaataatcgc tatacgcatc tgataacgat tgcacaggcc
     5161 caccatcaag aggctggaaa tcttgctgcc agtcctcacc aacaaaacag gttattggca
     5221 atatttcagc cagatgtttc gccagatcca catctccaga taagaggcaa aaaagagcta
     5281 tcgattcggg ttttatcgtt ttcatgcctc tcgccctcca cttaatttcc tgtaatggcg
     5341 gtctggttga taagccatga aacaatattg ccacaccttg aatgcagccc tggtgcctgt
     5401 cggcactgcg atgaaagaac taaatgatgc cgccataccc tgatcctttc agggtggtag
     5461 gggtataaag acgcgccagt actctaccgg acgcttccgg gcaacccact tcccaccgaa
     5521 gttgttggta ttgactgaca gactggtctt ctggcttagc gtcatcctca cccgaccttc
     5581 ccaatctcgc gatcagtggc attttcgggt ttgtcagcat tacagcagcg ggggctgcga
     5641 gggattttca ccctcttccc aggcacaaat gtgccacacc tgtcagcgta tgttatgagc
     5701 tgatttcact tttcccataa cacgtaaata acgttaatta ttcattccag cgtttattta
     5761 aaccttccct ttttttagca tttattctat accgtttcca tcgccgcatt ttcactcact
     5821 tgctgtcgat acacctgcgg cgtaacctga taagtctgac ggaaaacttt gcaaaaataa
     5881 ctgtattgtc gtggggatta ggcgcacgag tctggcgcac cgaatctgac cgagatttgt
     5941 tctctttaat aaccacaaaa ttgagttgtt ttttgaggca attttccaca atcaacttca
     6001 acaaatcagc agaggccata actcttgagt aatccatctc aggaactttg cggaattcat
     6061 taagtaattc gggatctgcc tgccagcaat catcgacatt cagaatatcg accaaatcga
     6121 catcatcatt acgcagacga acttgcccgc acaatacaaa accgactaaa tgtccggcga
     6181 ttaccagtgg aatagagaaa tcggttaatc cggcatgaca gcgataaata caaggctgat
     6241 cggatttaga ggcttccagg cccccacaac gatcgctcat gcgacatcga gtgcttagct
     6301 gagggtgttg ccgcatcatc tgacaaaatg gagtgaagtt aaacagctcg gaaatttcat
     6361 cgccgtgaat attgaccacg acaaccgcaa ggctggtcgc ctgcgcaaaa tcctgcgcga
     6421 ttttattaat gagttctgaa ttaagtgtcg ccgcagaaat catgataaaa cctcccagtt
     6481 aatttattgt tatagcaaaa caattgtttt acatttaatc ttcacatcat ccctgatgat
     6541 ttactgacga gaagcacaaa ggacaataac aaaagcctcc gtgaagcggg agacttttta
     6601 ataagttcgg aaagcagatc acaaacagta aaaaaataaa tgtttataac tgccagagta
     6661 tttcgttatt cgctaattcc tttaggtgta acactattga caggtaaatt ctttgcaatg
     6721 agatagcgat aaatcgccgc accaacacat gccccaatga tcggtgcaat aatgggtaca
     6781 atgaaataag gaatattgcg tccaccagtc atcgcaattt ctccccaccc cgcaagccag
     6841 gtgaaaatct taggtccaaa atcacgcgct ggattcatgg caaaaccggt taatggtccg
     6901 gtagaagcac caataacagc aaccagaata ccaataagta aaggtgcaag cggtcctttg
     6961 ggtacgccat taccgtcatc ggtcaacgcc ataatcaacc ccattaacat ggacgttatg
     7021 accacttcga ccaacgcagc ttgccataca ttaatcgccg ctgccggata agtactgaag
     7081 atactggcga gttgtaaact ttctgcgcta ccacgcacca tattgtgagc agattcaaaa
     7141 tcggtaaaca gactactgta tagagaatat gccagaactg caccaccaaa ggcacccgct
     7201 aattgcgcca gacaaaatgg cagcacttta cgggctggga aagaggcaaa cagccacaga
     7261 gcaatggtaa ctgccgggtt gagatgagcg ccggaaatac ctgccgtaag gtaaacagca
     7321 agcgaaatcc ctaaccccca aatgatacaa atttcccaca gtcccagact tgccccggca
     7381 actttcaggg cgcacaaaca accaatgcca aaaaagagga acagtccggt gcccaaaaac
     7441 tcggcaacgc attgcgcctt gagtgaatca ttcatagtga cacctttttt acggagtttt
     7501 tctttatcag ccaactatgc gctgactgtt gtatataccg agcacaacta taaaaaattg
     7561 ctctgcagaa aatattgcca acatgcatga cagactcgtg taaaaatcgt gctaacataa
     7621 caaattattg tcattaccga atcatgcaac acagcgttag tcagtgttga aagtaataat
     7681 tcccaccaaa cttttacaat aaaaaattgc catcatgaca taataaaatt gaaacaaata
     7741 tcacagttcc ttcttcgaac taaactcaac caaatattac tcgcgattaa actcactagc
     7801 caacgcaatt aattcacatt
//
LOCUS       DAZPDU010000194         7774 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZPDU010000194 DAZPDU010000000
VERSION     DAZPDU010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7774
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.196"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..1780)
                     /locus_tag="ACHURS_003834"
     CDS             complement(<1..1780)
                     /locus_tag="ACHURS_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HFR1239457.1"
                     /translation="MEKHGAELLLQRMLSNTSATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGPTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLRGGVDCLLISPERLANQDFLETANTPVLGTTATANNRVVEDIRQQLGDI
                     VIQRGTLARESLALDALVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDAELVAEWLR
                     TNGISAFAYYSGVTCEGAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFV
                     IHYQMPGSIVGYYQQVGRAGRAIDSAVGILLCGGEDRAIHKFFRESAFPAEAQIHEIL
                     NVLSENDGLTLRGIEQRTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDH
                     KRINHLMNQRKNELADVESYITTKECKMQFLRRALDEPSAERCGKCSSCLQHPLLSPD
                     IDSGLLHAANLFIKHADLPLNLNKQVASGAFTQYGFKGNLPAGLQGSTGRVLSRWGDS
                     GWGKQVAQEKKTGRFSDELVEACAEMVRQRWNPHPEPTWVCCVPSLKHLDLVPDFARR
                     LAAKLGLPFIDAIEKVVDNPPQKMQQN"
     gene            complement(1921..3261)
                     /gene="gntU"
                     /locus_tag="ACHURS_003835"
     CDS             complement(1921..3261)
                     /gene="gntU"
                     /locus_tag="ACHURS_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFR1239458.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTDTNLVKLIIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNNSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(3265..3792)
                     /gene="gntK"
                     /locus_tag="ACHURS_003836"
     CDS             complement(3265..3792)
                     /gene="gntK"
                     /locus_tag="ACHURS_003836"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFR1239459.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(3931..4926)
                     /gene="gntR"
                     /locus_tag="ACHURS_003837"
     CDS             complement(3931..4926)
                     /gene="gntR"
                     /locus_tag="ACHURS_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFR1239460.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(5150..5845)
                     /gene="yhhW"
                     /locus_tag="ACHURS_003838"
     CDS             complement(5150..5845)
                     /gene="yhhW"
                     /locus_tag="ACHURS_003838"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFR1239461.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(5968..7005)
                     /locus_tag="ACHURS_003839"
     CDS             complement(5968..7005)
                     /locus_tag="ACHURS_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFR1239462.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDICSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            7339..>7774
                     /gene="yhhY"
                     /locus_tag="ACHURS_003840"
     CDS             7339..>7774
                     /gene="yhhY"
                     /locus_tag="ACHURS_003840"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFR1239463.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQHPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKY"
BASE COUNT         1976 a         2101 c         1872 g         1825 t
ORIGIN      
        1 ggttttgctg cattttctgc ggtggattgt ccacgacttt ttcaatggca tcaataaaag
       61 gtaagccaag tttcgccgcc agtcgccggg caaaatcagg aaccaggtcg aggtgcttta
      121 atgaaggaac gcagcatacc caggttggtt caggatgcgg attccagcgt tggcgaacca
      181 tttccgcaca tgcctccacc agctcatcac tgaatcgccc cgtttttttc tcctgtgcta
      241 cctgctttcc ccacccggaa tctccccaac gagaaaggac tcgccccgta gatccttgta
      301 aacccgcagg caagttccct ttaaatccgt attgcgtaaa agctccagat gctacctgtt
      361 tattgagatt taatggcagg tcagcgtgtt taataaataa atttgccgca tggagtaagc
      421 cgctatcaat atcgggcgac aacaacggat gctgaagaca actgctacat ttaccacaac
      481 gttcggcact tggctcatcg agtgcgcggc gcagaaattg cattttgcac tccttggtcg
      541 tgatatagct ttcaacatct gccagctcat tttttctctg attcatcaaa tggttaattc
      601 gtttatggtc aggagagaaa ctgacgatag ttctgcgcca taatttctcg gtatacacca
      661 caggcgatgg attttcagct accaataatt tcagtgcttt ttctatttgc ccataacgga
      721 gattcgtccg ttgttcaatg cctcgtaggg taagaccgtc attctcgcta agtacgttga
      781 gtatttcatg aatttgcgcc tccgcaggaa aggcgctttc acggaagaat ttatgaatag
      841 cgcggtcttc accaccacaa agcaatatgc caactgcgga atctatagca cgcccggcac
      901 gccctacctg ttggtagtaa ccgacaatag accccggcat ttgataatga atgacgaaac
      961 ctaaatcagg tttatcaaac cccataccta atgccgtcgt cgcgaccagc actttgattt
     1021 tatttgccag cagcgcctgc tccagatatt ccctggcagt attagaatct tccgcgcctt
     1081 cacaagtcac gccgctgtag taagcaaatg cactgatacc atttgtcctt aaccactcgg
     1141 cgacaagttc ggcatcgcga gtagtcaggg tataaacaat tcccgatttg gaaaactgag
     1201 ggataaccgt tgctaaccat gccagacggg atgactgttc acctaagact aaggcatcaa
     1261 gcgccaaact ttcgcgagcc agcgttccac gctgaatcac aatgtcaccc aattgctgac
     1321 ggatatcctc aacgacacga ttattcgctg tcgcggttgt acccagaaca ggggtattcg
     1381 cagtttcaag aaaatcctga ttcgccaacc gttcaggtga aatcagcaga caatctacac
     1441 caccccgcag caacttatcg ctaatgcgct gccattcttc cctgtttgta gaattcaacg
     1501 tttcagccgt aatacctaac cgttcagcgg cggcaacctg gttacgcatc agggcaagca
     1561 aaggagagat gataattgtc gggccagcgc cgcgatcgcg gaagatttta ctggcgatga
     1621 agtacacggc acttttcccc cagccagtgc gttggacaac cagcagtttt cgccgctgat
     1681 taaccaccgc atctatagct tcccattgcc cttccctgaa agtagcgctg gtgttactta
     1741 acatccgctg taacagcaat tcagctccgt gtttctccat catcttctcc ctcgtccgtt
     1801 aacgcagtga ttagaacgga taatgttccg gtctttttgc gagagagatc aaaaagatga
     1861 agacaggaga taagagaaat gaattactgc gagcaagctc gcagtaattt gtcgcagtaa
     1921 ttaactcaac agctggaacg caatcatccc aacaatggca ccaacagtgc cgaggatggt
     1981 ttccatcatg gtccaggttt tcagcgtttc ggcttcggtc gcgccggtaa atttaccgaa
     2041 caaccagaaa ccagcgtcgt taacgtggct gacaacaatc gaaccaccgg cgatacaaat
     2101 cgacagcgcc gccatttgcg caccggaatt gttcagttgt tcaatgaccg gcatcaccag
     2161 ccccaccgcc gttaaacagg caacggtggc agacccctga atgatgcgca ctgcagctgc
     2221 cagcacgaag caggtgatag caatcggcag gcccatgccg gttaacgctt cgcccagtgc
     2281 cggacctacg ccagagtcaa ccagcacctg tttgaacacg ccgcctgcac caatcaccag
     2341 cagaatgatc cccgccggtt gcagcgcgtg accgcaaatc tccatcactt tgtcttttgg
     2401 catgccctga cgcattgcca ggccataaat cgctaccaga caagcaacca gaatcgcggt
     2461 aaacggatga ccaataaact cgaaccattc gtaagcggta gagccttctg gcacaaaacg
     2521 cgcggcaatg gttttcagcc ccaccagcac cagcggcaac aggatcagcg acaggctgaa
     2581 tccgaaagat ggcattttac cttcgccgag atgcggctcg ctgatgtcgt caggaatatg
     2641 cagctcaacg taacggctga tgaagttacc ccacagcggc ccggcaataa tcattcccgg
     2701 aattgccgca cacaggccaa tcaggatcat ccagccaaaa tcggcgttca tctgcgatgc
     2761 cagcagcatt ggcgctggcc ctggcaccag gaacgccgcc gctgccgcca cgcctgcgaa
     2821 taatgggatt atcagcttca ccaggttcgt atcggtgtgg cgcgccattg agaaagcaac
     2881 gctaatcagc agaacaatcg ccacttcaaa gaacagcggt agcgcacaga ccagccccgc
     2941 aaggccgatg gcataatgcg cgcggctgtg accgaaggat ttgagcattt tgacggcaat
     3001 ctgatcgact gcgccggttt catgtaagat cttgccaaac atagctccca gggcgacaac
     3061 caccgccagg aagccgaggg tgcctcccat ccctttttcc atcgtcgctg cgattttatc
     3121 gagcggcata ccggaaaaaa ggccagcccc catggacacc accatcaaag ccaggaaagc
     3181 gtgcatacgc gccttcatga ctaaaaacag cagcagtaaa acagacccta ctgctgttaa
     3241 aacaagcgtt aatgtagtca ctacttattt gcctttttta ataacctcaa tggtgcttgc
     3301 cacaacacct tccagcggtt gatcgatatc caccaccagt acatcggttt cgtccgcacc
     3361 cggctcctgc agcgtttcaa actgcgtcac caacatttgg gttttaaaga aatggccttt
     3421 gcgcgctttc aggcggcttt caatcacgtc aaaatcgcct ttcaggtaga tgaaagagag
     3481 attcggatta ccttcacgca gcaagtcgcg atagtgtttt ttcaatgcag aacagacgat
     3541 cagtgacact ttattagtgc gctgcatagc aaacgcggcg tcgttcagcg cctgcaacca
     3601 gggtttgcga tcgtcgtcat tcagcggttc gccagacgcc attttttcga tattgcgccg
     3661 tggatggagg aaatcgccat caagaaacgc ggcatgaagt tgatgcgcca cttcactggc
     3721 gaccgcagat ttgccgctgc ccgatacgcc catcaagacg taaatgtggt gatcatggtt
     3781 agtcgtgctc aaagtggtgc ccccacaata caagaattaa aaatgttacg ggtaactgtt
     3841 atcggtaaca ttgtccagcc ggacaatatc agaagcaata tccatccgtg ccttaagtgt
     3901 ataagtgtga gctacttcaa atttgtaggc ttaaatagat ccgcccggtg acaaggtgaa
     3961 acctaaatct aacattttcg gtgtcacaga ttcgccacga atacgcgcca gcaggcgttc
     4021 agcgccaata ctgcccatcc gctcacgcgg cgtcagcacg ctcgctaacc gcggctccat
     4081 cacctggcca atgtcatgac cgtggaaacc ggcaatcgcc atatcgtcag gaaccttcaa
     4141 tcccagacgc tgacattcaa acgccgcgcc gaccgccagg tcatcgttgg tacagaacac
     4201 gccatccagc tgcggatatt cccgccgcgc ctggcgaatc agttcaatac cggaagagta
     4261 agaagaagat tgctcaacca tcacgctata cggcaccaga cctgcatcca gcatcgcctg
     4321 ttcgtatccc ttctgtttga tgatagtacg ttcgtcgaga cgcgcgccga gataggcaat
     4381 gtggcaatgc ccgcgagcaa taatggcggt ggtcatctgg cgtgccgctt caaagttatc
     4441 aaaaccgacg gcgatatcga ggcacggcga ctggctgtcc atgagttcca ccaccgggat
     4501 ccccgccact tcaatcatct ttaaggtgcg cggcgtgtgg gtacgttcgg tgaggatcag
     4561 tccgtcgata ttccaggaga gcatggattc gagacgttct tgctccattt ccggtttata
     4621 accgtagtga gccagcatgg tctgataacc gtgcgcgtcg gtgacgcttt cgattccgcg
     4681 taatacttcc gcgaaaacct ggttggtgag agaaggtaac aggacgccaa tcgcccggct
     4741 ggtggcgtta gagaggatat cgggcgcacg attgggaata tagcccagtt catcaagagc
     4801 ggcggcaatc ttgccgcgta gagcgacgga aacctgctcc gggttgcgta aaaaacggct
     4861 gaccgtcatt ttggtcacgc ctacacggtc agccacatcc tgaagtacgg gtcttttctt
     4921 tttcatcgtc ctgaaggtac aaaagagata gattctctta gtttaacacg gacgcatcag
     4981 aaccttcccg gggaaaacag tgcttgttta caattattag atatacacta aatcaattgc
     5041 atcgcattgt gctaacgacg cggtaaaacc ccggaagcgt gcatcagcac gcgaccggag
     5101 cgagcgagga agcctgcaaa aaggcaggct tcaaagatgc cgttgagttt taaaccggcg
     5161 gcagatcgaa cagtaacact tcgctgtcgc tatccgcatg gatggagatt gcctgctcat
     5221 cccagattgc cagaccatcg ctggtcgagg ctttcacgcc gttaatggtg acattgcctt
     5281 tcaccacctg gatccagacg cggcgttcag cggcaatctg atgcaccgac tgctcatctt
     5341 tcagcaacgc ccagcggtac agttccatat cctgatgcac tttcaatgaa ccatctcgcg
     5401 catccggcga gagcaccagc tgtttgccct gtacggcatc gaagcgacgc tgttcataac
     5461 gcggcgtaat accgttttct tcaggcatga tccagatctg atacagatgc agacgctcgg
     5521 tgctgcttgg gttgtactct gagtgacgaa tacccgtacc cgcactcata atctggaact
     5581 cacccgccgg aacctgctct ttattgccca tgctgtcctg atgctcaaca gtaccttcca
     5641 gcacgtaggt caaaatttcc atatctttat gcgggtgagt gccgaagccc tgccctgctt
     5701 caatcacgtc gtcgttaatc acgcgcagcg cggagaagcc cataaagttc ggatcgtaat
     5761 agttggcaaa agagaaagta tgccaggagt ccagccagcc atgatttgca tgaccacgtt
     5821 catttgcttt gcgtaagtag atcatttgta ttcgccccat gaatgatttc gatgggctta
     5881 gtgtggaccc gatctgcctg ggatgataga gggtgaaaat tgacccctct gttcaaaaaa
     5941 attgaactat tcgaggggcc atcaaactta cttcgcgaga gtcactgtgg agggagaggc
     6001 ttgctcaaat ccgcgttcaa ggatttccag attggtaaga acttcagatt ccttgacgta
     6061 atttggcgca ccgtgggtga tggtttgata caacgcatca taaacgcgcc cgtaatcgcc
     6121 catctccggc ttcatctctt ctctgaccgt cacgccctcg tcattgacat actccagcac
     6181 accgaccgaa tcatccgctg cgaatcccgg ttcgcccggc ataatattag ccttcaggct
     6241 ggtttcctgc tggtcgatac cgtatttaat aaacgaacct ttcttaccgt gaacgataaa
     6301 tttcggataa tcgattttca ccagatggct ggttttgacg atggctttca ggtcgccata
     6361 aaacagttgc gcttcaaagg tgtcgtcagg attggcttta ttacgcaggc tgcagatgtc
     6421 ataagcgacg tgatccgggc gaccgaacag agaaataatc tggtccatcg tatgcacacc
     6481 aaggccatag aacgcgccat cctgcggcaa cccaggtttg gtttctgcca ccgggcggta
     6541 atagtcaaaa tggctttcca cttcaacaat ctctcccagc ttaccgcttt caatcgcttt
     6601 tttcgccgtc aggaagcagg aatcaaagcg acgattctga tatggcgtga cggtcagccc
     6661 tttgcttttc gccagtgcaa acagctcttt cgcctgcgca agtgtcggag tgaacggttt
     6721 ttcgaccagc acatttttcc cggcttccag cgcgcgcttc gcgtactcga agtggctgtc
     6781 cgcatgggtg cagacaacaa ccaacttaac atcgggatcg tttagcactt cgtcgagatc
     6841 gctggtgaaa tggatatgag agtaaatggg agcctgttct tccggcttcg catggcgacg
     6901 aaaaatatgc gcgacatgcc agctatcctt gcggttaagt acatacggca gatggtaacg
     6961 ggtggtgctt ttgccgaagc caataaaggc gcagttgatg accatgattt cgtccttttt
     7021 aaggtggtta tttacacctt agcgcaaagc ggacgtggtt cctactggag tgcgcataac
     7081 gaacacaagc actcccgtgg ataaattgag aacgaaagat caaaaaaaaa gccagcaccc
     7141 ggctggctaa gtaatactgg aagcaatgtg agcaatgtcg tgctttcagg ttctccgcga
     7201 gggtcttcct gatcgcgaga caataataat cattctcatt cgcacttgtc caacactttt
     7261 tgcaaaaaaa atgcatttga ctcgcatttg aaagtcaatg atgttgaaag ggacatttac
     7321 cccaaagagg acaaaggaat gagtgagata gtaatacgcc acgcagaaac acgggattac
     7381 gaggccatca ggcagattca cgcccagccg gaggtgtatt gcaacacact acaggtgcct
     7441 catccttccg atcatatgtg gcaggagcga ctcgccgatc gtcccggcat caagcaactc
     7501 gtcgcctgta ttgatggaga cgtcgtgggc catctcacca ttgacgtgca acagcaccca
     7561 cgccgcagtc atgttgccga ttttggtatc tgtgtcgact ctcgttggaa gaaccgcggc
     7621 gtcgccagcg ccctgatgcg agagatgatt gaaatgtgcg acaactggtt gcgggtagat
     7681 cgcattgaac taaccgtgtt tgtcgataac gccccggcaa ttaaggtcta taaaaaatac
     7741 ggctttgaaa ttgaagggac tggtaagaag tacg
//
LOCUS       DAZPDU010000195         7718 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZPDU010000195 DAZPDU010000000
VERSION     DAZPDU010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7718
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.197"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..643
                     /locus_tag="ACHURS_003841"
     CDS             <1..643
                     /locus_tag="ACHURS_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1239464.1"
                     /translation="HAFITESDADGHVLTVREDLIWPLLEVLYPGTRETFGLPGICLS
                     LADKPDELAALEHYWQLIERESVEQLPGREHTLTLLAQAVFTLLLRNAKLDDHAASGM
                     RGELKLFQRFNMLIESHFHQHWTVPDYANELHITESRLTDICRRFANRPPKRLIFDRQ
                     LREAKRLLLFSDNAVNNIAWQLGFKDPAYFARFFNRLVGCSPSAYRAKKVPVT"
     gene            894..2456
                     /gene="hpaB"
                     /locus_tag="ACHURS_003842"
     CDS             894..2456
                     /gene="hpaB"
                     /locus_tag="ACHURS_003842"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HFR1239465.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDANGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            2474..2986
                     /locus_tag="ACHURS_003843"
     CDS             2474..2986
                     /locus_tag="ACHURS_003843"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HFR1239466.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            3379..5529
                     /gene="btsT"
                     /locus_tag="ACHURS_003844"
     CDS             3379..5529
                     /gene="btsT"
                     /locus_tag="ACHURS_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFR1239467.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGHLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            5579..5782
                     /locus_tag="ACHURS_003845"
     CDS             5579..5782
                     /locus_tag="ACHURS_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFR1239468.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            5793..6749
                     /gene="yjiA"
                     /locus_tag="ACHURS_003846"
     CDS             5793..6749
                     /gene="yjiA"
                     /locus_tag="ACHURS_003846"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFR1239469.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYEIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(6827..>7718)
                     /locus_tag="ACHURS_003847"
     CDS             complement(6827..>7718)
                     /locus_tag="ACHURS_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HFR1239470.1"
                     /translation="DRVTEIDRLLEQKPRLTADQAWDVIRQTSRQDLNLRLFLPTLQA
                     ATSGLTQSDPRRQLVDTLTRWDGINLLNDDGKTWQQPGSAILNVWLTSMLKRTVVAAV
                     PMPFDKWYSASGYETTQDGPTGSLNISVGAKILYEAVQGDKSPIPQAVDLFAGKPQQE
                     VVLAALEDTWETLSKRYGNNVSNWKTPAMALTFRANNFFGVPQAAAEETRHQAEYQNR
                     GTENDMIVFSPTTSDRPVLAWDVVAPGQSGFIAPDGTVDQHYEDQLKMYENFGRKSLW
                     LTKQDVEAHKESQEVLHVQR"
BASE COUNT         1782 a         2104 c         2022 g         1810 t
ORIGIN      
        1 tcatgcgttt attacggagt ctgacgccga cggtcatgtg ttgacggtac gggaagatct
       61 gatctggccc ctgctggaag ttctttatcc aggcactcgg gaaaccttcg gcctgccggg
      121 gatttgcctg tcactggcag ataaacccga cgaactggcg gcgctggaac actattggca
      181 actgatagag cgggaatcgg tggaacaact gcctggacgg gaacacaccc tgacgctact
      241 ggcacaggca gtgttcaccc tactgctgcg taatgcaaaa ctcgacgacc atgccgccag
      301 cgggatgcgc ggagaattaa aactgttcca gcgttttaat atgctgattg aaagccattt
      361 tcatcagcac tggacagtac cggattacgc taacgaactg catatcaccg aatcacgcct
      421 cacggacatc tgccgccgct ttgccaaccg tccgccaaaa cggttgattt tcgacaggca
      481 gctgcgagaa gccaagcggc tgctgctgtt ttctgataac gccgtgaaca atattgcctg
      541 gcaactcggt tttaaggatc cagcttattt tgcgcgcttt tttaatcgct tagtcggttg
      601 ctcgcccagt gcttatcgtg ccaaaaaagt acctgtgacg tgaaaatccc ttagccttaa
      661 cgggaaacca ggcaccacct gctattcccc tttctccgcc tggagcaagg ggatttagta
      721 acgtcaaaaa tcgctaaaag cgaccgcgat cacaaaaccg tacccgacct gaaaagtacc
      781 agccattgtc ccaaaagtgt cttcccgtaa tcgcccctgc aagcttcaat tgaataacaa
      841 aaacaacaca caaaattaac aatcgtattc cgattaatac tgtagaggtc gacatgaaac
      901 cagaagattt ccgcgccagt acccaacgtc cgttcaccgg ggaagagtat ctgaaaagcc
      961 tgcaggatgg tcgcgagatc tatatctatg gcgagcgagt gaaagacgtc actactcatc
     1021 cggcatttcg taatgcggca gcgtctgttg cccaactgta cgacgcgctg cacaaaccgg
     1081 agatgcagga ctctctgtgc tggaacaccg acaccggcag cggcggctat acccataaat
     1141 tcttccgcgt ggcgaaaagt gccgacgacc tgcgccagca acgtgacgcc atcgctgagt
     1201 ggtcacgcct gagctatggc tggatgggcc gtaccccaga ctacaaagct gctttcggtt
     1261 gcgcactggg cgcgaatccg ggcttttacg gtcagttcga gcagaacgcc cgtaactggt
     1321 acacccgtat tcaggaaact ggcctctact ttaaccacgc gattgttaac ccaccgatcg
     1381 atcgtcattt gccgaccgat aaagtgaaag acgtttacat caagctggaa aaagagactg
     1441 acgccgggat tatcgttagc ggtgcgaaag tggttgccac caactcggcg ctgactcact
     1501 acaacatgat tggcttcggc tcggcacaag taatgggcga aaacccggac ttcgcactga
     1561 tgttcgttgc gccaatggat gccaatggcg tcaaattaat ctcccgcgcc tcttatgaga
     1621 tggtcgcggg tgctaccggc tcgccgtacg actacccgct ctccagccgc ttcgatgaga
     1681 acgatgcgat tctggtgatg gataacgtgc tgatcccatg ggaaaacgta ctgatctacc
     1741 gcgatttcga tcgctgccgt cgctggacaa tggaaggcgg ttttgcccgt atgtatccgc
     1801 tgcaagcctg tgtgcgcctg gcagtgaaac tcgacttcat tacagcactg ctgaaaaaat
     1861 cactcgaatg taccggcacc ctggagttcc gtggtgtaca ggccgatctc ggtgaagtgg
     1921 tggcgtggcg caacaccttc tgggcattga gtgactcgat gtgttctgaa gcgacgccgt
     1981 gggtcaacgg ggcttattta ccggatcatg ccgcactgca aacctatcgc gtactggcac
     2041 caatggccta cgcgaagatc aaaaacatta tcgaacgcaa cgttaccagt ggcctgatct
     2101 atctcccttc cagtgcccgt gacctgaaca atccgcagat cgaccagtat ctggcgaagt
     2161 atgtgcgcgg ttcgaatggt atggatcacg tccagcgcat caagatcctc aaactgatgt
     2221 gggacgccat tggcagcgag tttggtggtc gccacgaact gtatgaaatc aactactccg
     2281 gtagccagga tgagattcgc ctacagtgtc tgcgccaggc acaaagctcc ggcaatatgg
     2341 acaagatgat ggcgatggtt gatcgctgcc tgtcggaata cgaccagaac ggctggactg
     2401 tgccgcactt gcacaacaac gacgatatca acatgctgga taagctgctg aaataacgca
     2461 gcaggaggtt aagatgcaat tagatgaaca gcgcctgcgc tttcgtgacg cgatggccag
     2521 cctgtcggca gcggtaaata ttatcaccac cgagggcgac gccggacaat gcgggattac
     2581 ggcaacggcc gtctgctcgg tcacggatac accaccatcg ctgatggtgt gcattaacgc
     2641 caacagtgcg atgaacccgg tttttcaggg caacggtaag ttgtgcgtca acgtcctcaa
     2701 ccatgagcag gaactgatgg cacgccactt cgcgggcatg acaggcatgg cgatggaaga
     2761 gcgttttagc ctctcatgct ggcaaaaagg tccgctggcg cagccggtgc tgaaaggttc
     2821 gctggccagt cttgaaggtg agatccgcga tgtgcaggca attggcacac atctggtgta
     2881 tctggtggag attaaaaaca tcatcctcag tgcagaaggt cacggactta tctactttaa
     2941 acgccgtttc catccggtga tgctggaaat ggaagctgcg atttaagctc cagccccttc
     3001 acgcattcgt cgaaggggca tttttattgt gatctcaatc acatatccac ccgatgaata
     3061 attcttgccc aaccgctcac cgccattcct gaccgcttac gtccctaaaa taaccactca
     3121 gttatttacc ttactttacg cgcgcgtaac tctggcaaca tcactgcagg atagcggtca
     3181 atttacctcc tcaaacgcaa cgcaaaccta gaacggcttc ggcctatgat taacaaatta
     3241 ctctatgaat tttggtttgc aggaaggcag caagtgagtg aatccccggg agcttacaac
     3301 agtaagtgac aggggtgaac gaacgcaact gccgcacctg taagccaaaa gacgacgagt
     3361 aaaatgccag gttttactat ggatacgaaa aaactattca agcacatacc ctgggtgatt
     3421 ctcggaatca tcggtgcatt ctgcctcgcg gtagttgcat tacgtcgggg ggagcacgtc
     3481 agcgccctgt ggatcgtggt cgcctctgtg tcggtgtatc tggtggcgta tcgctactac
     3541 agtctgtaca tcgcccagaa ggtgatgaaa ctcgacccca cgcgcgcgac gcctgcggtt
     3601 attaacaacg acggtctgaa ctacgttccg accaaccgtt acgtgttgtt tggtcaccac
     3661 ttcgccgcta tcgccggtgc tggtccgctg gtcggtccgg ttctcgccgc acagatgggc
     3721 cacctgcctg gcacgctgtg gctgctggcg ggggtagtac tggccggtgc ggttcaggac
     3781 tttatggtgc tgtttatctc ctctcgccgt aacggcgcat ctctcggtga gatgataaaa
     3841 gaagagatgg gaccagtacc ggggactatc gcgctgtttg gctgtttctt aatcatgatc
     3901 atcatccttg ccgtcctggc gctgattgtg gttaaagccc tggccgaaag tccgtggggt
     3961 gtcttcaccg tttgctcaac cgtaccgatt gcgctgttta tgggtatcta catgcgcttt
     4021 atccgtccgg ggcgtgtggg tgaagtctct gtcattggta tcgtgctgct ggttgcctct
     4081 atctacttcg gtggcgtgat tgctcacgat ccgtactggg gtccggcact gacctttaaa
     4141 gacaccacca ttaccttcgc gctgattggc tatgcgtttg tttccgcatt gctgccggtg
     4201 tggctgatcc tcgcaccgcg cgactatctg gcaaccttcc tgaaaatcgg cgttatcgtc
     4261 ggcctggcgc tgggtatcgt ggtgctgaac ccggaactga aaatgcctgc gatgacccag
     4321 tacattgacg gtactggccc gctgtggaaa ggtgctctgt tcccgttctt gttcattacc
     4381 atcgcctgtg gtgcggtatc tggcttccac gcactgatct cttccggtac gacgccgaaa
     4441 ctgctggcta atgaaaccga cgcgcgtttc attggctacg gcgcaatgct gatggagtcc
     4501 ttcgtggcga ttatggcgct ggttgctgcg tccatcatcg aaccgggtct ttacttcgcg
     4561 atgaacaccc cgcctgctgg ccttggcatc accatgccta acctgcatga aatgggtggc
     4621 gagaacgcgc cgatcatcat ggcgcagctg aaagacgtta ccgcacacgc ggcagcgacc
     4681 gtcagctctt ggggcttcgt gatttcgcca gagcagatcc tgcaaaccgc gaaagacatt
     4741 ggtgagcctt ctgtcctgaa ccgtgcaggt ggtgcgccaa cgctggcggt aggtatcgct
     4801 cacgtgttcc acaaagtgct gccgatggct gacatgggct tctggtatca cttcggtatt
     4861 ctgttcgaag ccctgttcat cctgaccgcg ctggatgcgg gtacccgttc tggccgtttt
     4921 atgctgcaag acctgctggg taacttcatc ccgttcctga aaaaaaccga ctctctggtt
     4981 gccggtatca tcggtactgc gggctgtgtg ggtctgtggg gctacctgct gtatcagggc
     5041 gtggtcgatc cgctgggcgg cgttaagagc ctgtggccgc tgttcggtat ctccaaccag
     5101 atgctggcag ccgtagcgct ggtactgggc accgttgtgc tgattaagat gaagcgcacc
     5161 caatacatct gggtaactgt tgttccggct gtatggctgc ttatctgcac cacctgggcg
     5221 ctgggtctga aactgttcag caccaacccg cagatggaag gcttcttcta catggcaagc
     5281 cagtacaaag agaagattgc taacggtact gacctgacgg cgcagcagat tgccaacatg
     5341 aaccacatcg ttgtgaacaa ctacaccaac gcaggcctga gtattctgtt cctgattgtg
     5401 gtgtacagca tcatcttcta cggtttcaaa acctggcttg cggtgcgtaa cagcgacaaa
     5461 cgtactgaca aagaaactcc gtacgttcca atcccggaag gcggcgtgaa gatctcttcg
     5521 caccactaac cgtgtttagc cccgcttcgg cggggctttg ttcttatcag agtgaactat
     5581 gtttggtaac ttaggacagg caaaaaaata tctcggccag gcggcgaaga tgttgattgg
     5641 cattccagac tacgacaact acgtcgagca tatgaagacc aaccatcccg acaagccgta
     5701 catgagctat gaagaattct tccgcgaacg ccagaacgcg cgctacggcg gcgatggtaa
     5761 aggcggtatg cgctgttgtt aatggagaga gcatgaaccc gattgcagtt accctactca
     5821 ccggtttttt aggcgcgggc aaaaccaccc tgctgcgcca tattcttaac gaacaacacg
     5881 gctacgagat tgccgtgatt gaaaacgaat tcggcgaagt ctccgttgat gatcaactga
     5941 ttggcgaccg cgccacgcag atcaaaacgc tgaccaacgg ctgcatctgc tgttcgcgct
     6001 ccaacgagct ggaagacgca ctactcgacc tgctggataa tctcgacaag ggcaatattc
     6061 agttcgaccg tctggtcatt gaatgcaccg gcatggccga tcccggcccg attattcaga
     6121 ccttcttctc ccatgagatt ttatgccagc gctacctgct ggacggggtg attgcgctgg
     6181 tggatgcggt acatgccgat gagcagatga accagttcac catcgcccag tcccaggttg
     6241 gctacgccga ccgtattctg ctgacgaaaa ccgacgtcgc aggcgaagca gagaaactgc
     6301 gtgaacgcct ggcgcgcatc aacgcccgcg caccggtcta caccgttacc cacggcgata
     6361 tcgacctggg ccttctgttc aacaccaacg gttttatgct ggaagaaaac gtcgtcagca
     6421 ccaaaccgcg tttccacttt atcgcggaca aacaaaacga tatttcgtcg attgtggtgg
     6481 aactggatta cccggtagat atcagcgaag tttcccgggt gatggaaaac ctgctgctgg
     6541 aatcggcgga taaactgctg cgttacaaag ggatgctgtg gattgacggc gaacctaacc
     6601 gcctgctgtt ccagggcgtc cagcgcctct acagcgccga ctgggaccgc ccgtggggcg
     6661 atgaaaaacc gcatagcacg atggtgttta tcgggattca gttgccggag gatgagattc
     6721 gggcggcgtt tgcgggactc aaaaagtaac cagccctcca acatcacaat acctgagact
     6781 ttatcaccct catattcctc cagaatatga gggcattcgg gcttaattat ctctgaacgt
     6841 gcaacacttc ctgcgactcc ttatgcgcct ccacatcctg cttcgttaac cagagcgact
     6901 tacgaccaaa attttcgtac attttcagct gatcttcata gtgctgatca actgttccat
     6961 cgggagcaat aaacccactc tgaccgggtg cgaccacatc ccaggcaagc accggacgat
     7021 cgcttgtcgt tggtgagaaa acaatcatat cgttttctgt tccacggttt tgatactccg
     7081 cctgatgacg cgtttcttcc gctgcggcct gcggtacacc aaagaaatta tttgcacgga
     7141 acgttaaggc cattgcaggt gttttccagt tactcacatt attgccatag cgtttggaaa
     7201 gagtctccca ggtatcttcc agcgcagcca acacaacctc ctgctgtggt ttcccagcaa
     7261 acagatcaac cgcctgtggg attggtgatt tgtctccctg caccgcctca tacaaaattt
     7321 ttgctccaac acttatattc agcgaaccag ttgggccgtc ctgggttgtt tcgtagccac
     7381 tggcgctgta ccacttatca aatggcatag gtacggcagc cactacggta cgcttcaaca
     7441 tactggtcag ccaaacgttc aggatggcag agcctggctg ctgccaggtt ttaccatcat
     7501 cattaagcaa attgatgcca tcccaacgtg ttaatgtatc caccaactga cggcgcggat
     7561 cgctctgtgt caaaccagat gtcgctgctt gcagagtagg taaaaaaagc ctcaggttaa
     7621 gatcctgacg actggtttgg cgaataacat cccatgcctg atcagcagtt aagcgtggct
     7681 tttgctcaag cagtcggtcg atctccgtaa cgcgatct
//
LOCUS       DAZPDU010000196         7643 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZPDU010000196 DAZPDU010000000
VERSION     DAZPDU010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7643
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.198"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..896)
                     /gene="waaH"
                     /locus_tag="ACHURS_003848"
     CDS             complement(<1..896)
                     /gene="waaH"
                     /locus_tag="ACHURS_003848"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFR1239471.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWKSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDIITRQRMIAEIFTSG"
     gene            complement(1135..2160)
                     /gene="tdh"
                     /locus_tag="ACHURS_003849"
     CDS             complement(1135..2160)
                     /gene="tdh"
                     /locus_tag="ACHURS_003849"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFR1239472.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(2170..3366)
                     /gene="kbl"
                     /locus_tag="ACHURS_003850"
     CDS             complement(2170..3366)
                     /gene="kbl"
                     /locus_tag="ACHURS_003850"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFR1239473.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANYPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            3580..4512
                     /gene="rfaD"
                     /locus_tag="ACHURS_003851"
     CDS             3580..4512
                     /gene="rfaD"
                     /locus_tag="ACHURS_003851"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFR1239474.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4522..5568
                     /gene="rfaF"
                     /locus_tag="ACHURS_003852"
     CDS             4522..5568
                     /gene="rfaF"
                     /locus_tag="ACHURS_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFR1239475.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDLRVLDKEAWPLMVERYVALAYDKGVMRTAQDLPKPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            5572..6543
                     /gene="rfaC"
                     /locus_tag="ACHURS_003853"
     CDS             5572..6543
                     /gene="rfaC"
                     /locus_tag="ACHURS_003853"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFR1239476.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKRFIEENAAMI"
     gene            complement(6613..>7643)
                     /locus_tag="ACHURS_003854"
     CDS             complement(6613..>7643)
                     /locus_tag="ACHURS_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFR1239477.1"
                     /translation="NIIWYSHYKVSGSVYTNAYRGPMETGKIALCSAFIFLVLFAKNE
                     LRTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDRVALSASHATTAGYIILFPSLL
                     ASILILKSDFRHKTTLYTINFMLSLCAVIVTETRAAILVFPFFALLLIVMDSYINKRI
                     NYKLYCFIAIALLAGVFSFKDTLLMRMNDLNNDLVNYSHDNTRTSVGARLAMYEVGLK
                     TYSPIGQSLEKRAEKIHELEEKEPRLSGALPYIDSHLHNDLIDTLSTRGIPGVVLTIL
                     AFSAILIYALRTAKEPYILILLFSLLVVGLSDVILFSKPVPTAVFVTIILLCAYFKAQ
                     SDQCLLDK"
BASE COUNT         2044 a         1878 c         1826 g         1895 t
ORIGIN      
        1 ataccggaag taaatatctc ggcaatcatc cgttggcggg taataatatc cggctctttg
       61 cgcaccgcat ggcaaacacg caatgcttcg taagttattt gctgatgaaa ttccggataa
      121 atcgtaattt tgtcggcata atttcgattt aatttctcta gcagacgagt aatcttaata
      181 tagtgacgtt gataattaag gtttttgttc ccttgtctat gcaaccgact cactgacgta
      241 ttatgcagat aatatttata taacgattgc tcggtatatc gcgcacgcag cgcattaaac
      301 ataaattctg ttgtccagac aatatcctga tgatgtaatc cggcaataaa tttaatgtta
      361 tttttaacaa taacatcgcg gcgataaacc cccatccaga caacgtgagt ccaacggcgc
      421 gaagaaagcc ccatccgcag ccagtccggg ccggttaata cgccggttga gcgaaggcga
      481 tcgctgggga tggatttcca ggtttctccc gtttcacgaa aacaccagtc agcgttgcac
      541 tgcgccacgt cgaggtcgtc ctctaacgcc atggtcatca gcgtttcgta catggtggga
      601 tagacttcat cgtcagcatc gacaaaagcg acatatttgc ccgttgccac ttcaatccca
      661 cgattacgcg ccaccgatgc gccagcattc gcctgatgca acaaacgaac gtgcggatag
      721 ttttctgcgt aatgctttgc tatttcaaca gaattatccg ttgaaccatc gttaataata
      781 atgatttcca gagcagtcca ggtttgcgta attaaagatt ccatacaagt gcggaaatca
      841 tcgcccgcat tatataacgg aataataaca ctaagtttat tggtgctgtt catcataaat
      901 taccgacgac ctctgaagtt atcctgacgt tttacaggct ttgtattaag aaactattaa
      961 gcctgtgaga tattattttt acaggtgaga gagtcacttc agggtagaga gataatgaga
     1021 gcagctatat aagagatcag cgcaattacg atatctttat tacactgaca ggattttaca
     1081 tatttaacgg attatcctca ggcaaaaggg ctggaatacc agcccttgtt cgtgttaatc
     1141 ccagctcaga attactttcc cggactggcc cgaacgcata gcgtcaaagc ccttctggaa
     1201 atcatcgata gagaaacgat gggtaatgat cggcgagaga tccagacctg actgaatcag
     1261 cgccgccatc ttgtaccagg tttcaaacat ctcacgaccg taaatacctt taatgaacaa
     1321 gcctttaaag attactttgg tccagtcgat agacatatcg gacggtggaa tacccagcat
     1381 cgcaatgcga ccgccgtggt tcatggtgtc gagcatggta cgaaacgctg gcggcgcacc
     1441 ggacatttcc agaccgacat caaagccttc ggtcatgccc agttctgcca tcacatcatt
     1501 aaggttttct ttagcgacgt tgaccgcacg ggtgataccc attttgcgcg ccagttcgag
     1561 acgatattcg ttaacatcag tgatcaccac attgcgtgca ccaacgtgtt tcgccaccgc
     1621 cgctgccata ataccaatcg ggcctgcacc agagaccagc acatcctcgc caaccagatc
     1681 gaacgacagc gcggtatgca cggcgttacc gaagggatca aaaatggaag ccaggtcgtc
     1741 ggagatattg tcgggaattt tgaaggcgtt gaacgccgga atcaccagat attcggcgaa
     1801 gcagcccggg cggttaacgc cgacgccgat cgtgttgcgg cacagatggg tacgaccgcc
     1861 acgacagttg cgacagtgac cgcaggtgat atggccttcg ccagaaacgc gatcgccaat
     1921 cttgaagccc ttcacttcct gaccaatacc taccacttca ccgacatatt catggcccac
     1981 gaccatcggc accgggatgg ttttttgcga ccactcatcc cagttataga tgtgaacgtc
     2041 agtcccgcag atggctgttt tacggatttt aatcagcaga tcgttatgcc cgagttccgg
     2101 tacaggaacg tcggtcatcc agatgccctc ttccgctttc agtttggata acgctttcat
     2161 ctcacatcct caggcaataa cgcccagttg tttaccaata cgcgtgaatg cttctactgc
     2221 acgcgtaatt tgctcagggg tatgcgccgc agacatctgg gtacgaatac gcgcctgacc
     2281 tttcggaacg accggataga agaaaccggt gacgtaaatg ccctcttttt gcagctcacg
     2341 ggcaaatttc tgcgccacta ccgcatcacc aagcatgacc ggaataatgg cgtgatcggc
     2401 tcccgccagg gtaaagcccg ccgccgacat ttgctcacgg aactgacgcg cgttcgccca
     2461 cagacggtca cgcagctcgc tacccgcttc gaccatctcc agtactttga tggatgcggc
     2521 aacaatggcc ggtgccagcg agttggaaaa caggtacgga cgagaacgct ggcgcagcca
     2581 ctcaaccact tctttgcgcg ccgcggtata accaccagaa gccccgccca gcgctttacc
     2641 gagcgtgccg gtgataatat cgacccggcc catcacatcg cagtattcat gggaaccacg
     2701 accattttca ccgacaaaac cgaccgcgtg ggagtcgtct accatcacca gggcatcata
     2761 tttatctgcg agatcgcaaa cgcccttcag gttggcaatc acgccgtcca tcgagaacac
     2821 accatcggtg gcaatcagca catgacgcgc accggcttca cgcgcttctt tcagacgtgc
     2881 ttccagctcc tgcatatcgt tgttggcata gcgatagcgt ttagctttac acagacgcac
     2941 accatcaata atagacgcgt ggttcagtgc gtcggagata atggcgtctt ccgcgcccag
     3001 cagcgtttca aacaggccac cgttagcatc aaagcaggaa gagtagagaa tcgcatcttc
     3061 catccccagg aatgccgcca gtttttgttc aagctcttta tggctgtcct gagtgccgca
     3121 aataaaacgc accgaagcca tgccgaaacc gtgagaatcc attcccgcct ttgccgccgc
     3181 aatcagatcc ggataattcg ccagcccgag atagttattg gcacaaaagt taatgacgtg
     3241 gcttccatca gccacagtga tatctgcttg ctgcgcagac gtaataatgc gctcttcttt
     3301 aaacaaccct tccgcccgtg cggtttccag atcgttggtt aactgctgat aaaattctcc
     3361 acgcattgcg attctccaga cagggcaaat tccagcacat attacccaaa cttataggtc
     3421 gcgacgagat gacgcgttaa cacttctgca aaattcagga taaataacgt ccatcaatct
     3481 cacttttggc gaatggctga agccaacgcc gtgtagtgat atgatagaag tattcgtgtc
     3541 tgagattgtc tctgactcca taattcgaag gttacagtta tgatcatcgt taccggcggc
     3601 gcgggcttta tcggcagcaa catcgttaag gccctgaatg ataaaggcat caccgatatt
     3661 ctggtggtgg acaacctgaa agacggcacc aagtttgtga acctggtgga tctggatatc
     3721 gcggactata tggataagga agacttcctg atccagatta tggctggcga agagttcggc
     3781 gatgtcgaag cgattttcca cgaaggcgcg tgctcttcca ccaccgagtg ggatggcaag
     3841 tatatgatgg ataacaacta tcaatactcc aaagagctgc tgcactactg tctggagcgc
     3901 gaaatcccgt tcctgtatgc ctcttccgca gccacctacg gcggacgcac ctccgacttt
     3961 attgaatccc gcgagtacga aaaaccgttg aacgtctacg gttactcaaa attcctgttt
     4021 gatgaatatg ttcgccaaat cctgcctgaa gctaactcgc agattgttgg cttccgctat
     4081 ttcaacgttt atggaccgcg tgaaggccat aaaggcagca tggcgagcgt cgctttccat
     4141 ctcaacaccc agcttaacaa cggtgaatca ccgaagctgt ttgaaggtag cgagaacttc
     4201 aaacgcgact tcgtctatgt gggcgacgtg gctgatgtga atctgtggtt cctggaaaat
     4261 ggcgtttccg gcatcttcaa cctcggtact ggtcgtgcgg aatccttcca ggctgtagct
     4321 gatgctacgc tggcttatca caagaaaggc cagatcgaat acattccgtt cccggataag
     4381 ctgaaaggcc gctaccaggc gttcactcag gcagatctga caaatctgcg cgcggcgggt
     4441 tacgacaaac cgttcaaaac cgttgctgaa ggtgtaacgg aatacatggc ctggctgaat
     4501 cgcgacgcat aagagctctg catgaaaata ctggtgatcg gcccgtcttg ggttggcgac
     4561 atgatgatgt cgcaaagtct ctatcgcacg ctccaggcgc gctatcccca ggcgataatc
     4621 gatgtgatgg caccggcatg gtgccgtcca ttattatcgc ggatgccgga agttaacgaa
     4681 gctattccta tgcctctcgg tcacggagcg ctggaaatcg gcgaacgccg caaactgggt
     4741 catagcctgc gtgaaaagcg ctacgaccgc gcctacgtct tacccaactc cttcaaatct
     4801 gcattagtgc ctttcttcgc gggtattcct catcgcaccg gctggcgcgg cgagatgcgc
     4861 tacggtttac tcaacgattt acgcgtgctc gataaagaag cctggccgct aatggtggaa
     4921 cgctatgtcg cgctggccta tgacaaaggc gtgatgcgta ccgcacaaga tctaccaaaa
     4981 ccgttgttat ggccgcaatt gcaggtgagc gaaggtgaaa aatcatatac ctgtaatcaa
     5041 ttttcgcttt catcagaacg tccgatgatt ggtttttgcc cgggtgcgga gtttggtccg
     5101 gcaaaacgct ggccacacta ccactatgcg gaactggcaa agcagctgat tgatgaaggt
     5161 tatcaggtgg ttctgtttgg ctcggcgaaa gatcatgaag cgggcaatga gattcttgcc
     5221 gctttgaata ccgagcagca ggcatggtgt cggaacctgg cgggggaaac acagcttgat
     5281 caagcggtta tcctgattgc agcctgtaaa gccattgtca ctaacgattc tggcctgatg
     5341 cacgttgcgg cggcgctcaa tcgtccgctg gttgccctgt atggtccgag tagcccggac
     5401 ttcacaccgc cgctatccca taaagcgcgc gtgatccgcc tgattaccgg ctatcacaaa
     5461 gtgcgtaaag gtgacgctgc ggagggttat caccagagct taatcgacat tactccccag
     5521 cgcgtactgg aagaactcaa cgcgctattg ttacaagagg aagcctgacg gatgcgggtt
     5581 ttgatcgtta aaacatcgtc gatgggcgat gttctccata cgttgcccgc actcactgat
     5641 gcccagcagg caatcccagg gattaagttt gactgggtgg tggaagaagg gttcgcacag
     5701 attccttcct ggcacgccgc cgttgagcga gttattcctg tggcaatacg tcgctggcgt
     5761 aaagcctggt tctcggcccc cataaaagcg gaacgcaaag cgtttcgtga agcgctacaa
     5821 gcagagaact atgacgcagt tatcgacgct caggggctgg taaaaagcgc ggcgctggtg
     5881 acgcgtctgg cgcatggcgt aaagcatggc atggactggc aaaccgctcg cgaaccttta
     5941 gccagtctgt tttacaatcg taagcatcat attgcaaaac agcagcacgc cgtagaacgc
     6001 acccgcgaac tgtttgccaa aagtctgggc tatagcaaac cgcaaaccca gggcgattat
     6061 gctattgcac agcatttcct gacgaacctg cctacagatg ctggcgaata tgccgtattt
     6121 cttcatgcga cgacccgtga tgataaacac tggccggaag aacactggcg agaattgatt
     6181 ggtttactgg ctgattcagg aatacggatt aaacttccgt ggggcgcgcc gcatgaggaa
     6241 gagcgggcga aacgactggc ggaaggattt gcttatgttg aagtattgcc gaagatgagt
     6301 ctggaaggcg ttgcccgcgt gctggccggg gctaaatttg tagtgtcggt ggatacgggg
     6361 ttaagccatt taacggcggc actggataga cccaatatca cggtttatgg accaaccgat
     6421 ccgggattaa ttggtggata tgggaagaat cagatggttt gtagggctcc ggggaatgag
     6481 ttgtctcaat tgacagcaaa tgctgttaag cgattcattg aagaaaacgc tgctatgatt
     6541 taaattacaa ccccttaata gataattttg aaataaaatc agcctccttg ctgattttat
     6601 ttcacatatt cattatttat ctaataaaca ttggtctgat tgtgctttaa aataagcaca
     6661 aagcaatatt atggtgacaa acacagcagt cggaaccggt ttagaaaaga gtattacatc
     6721 acttaggcct actaccagta gtgaaaaaag caagattaaa atataaggtt ctttagcagt
     6781 tcttaaggca tatatgagta ttgctgaaaa ggctaaaatt gttaatacaa ctccaggaat
     6841 accacgcgtt gataacgtat ctatgagatc gttatgcaaa tgagaatcta tatagggtaa
     6901 agcgccactc aatctaggct ctttttcttc tagttcatgt attttttccg ctcgtttttc
     6961 cagtgattgt cctattggag aatatgtttt taagccaact tcatacattg ccagacgggc
     7021 accgactgaa gttctggtgt tatcatgcga ataattaact aaatcgttat ttaagtcatt
     7081 cattctcata agcaatgtat ctttaaaaga aaatacacct gctaataatg caatcgcaat
     7141 aaaacaatat aacttataat taattcgctt attaatataa ctatccatta cgattaatag
     7201 taacgcaaag aatggaaaca ctaatatggc tgcacgcgtt tcagttacta tgacagcaca
     7261 taagctaagc atgaagttaa ttgtatataa tgttgtttta tgtctaaagt cggattttaa
     7321 aatgagaatt gatgccagta aagaagggaa aaggatgatg tagcctgctg ttgtagcgtg
     7381 ggaagctgat aatgcaacac ggtcaacgtt taaatagaaa tgttgccaca tggcatgcgc
     7441 aaaaaaaagt aactgcgttg ccagggatgc gaacagaatt agtttcccaa attttatttt
     7501 tgttctcaac tcatttttag caaaaagaac caagaaaata aaagcactac acaaggcaat
     7561 tttcccagtt tccattgggc cacgatatgc attggtataa acagagcctg aaactttata
     7621 atgtgaatac cagatgatat tta
//
LOCUS       DAZPDU010000197         7611 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZPDU010000197 DAZPDU010000000
VERSION     DAZPDU010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7611
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.199"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            171..701
                     /locus_tag="ACHURS_003855"
     CDS             171..701
                     /locus_tag="ACHURS_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFR1239478.1"
                     /translation="MNDKELIKEIKERISSLDVRDNVERRAYEIALASLTAEPVAWKV
                     TFTQIDREYNTFTGMYSDKAEVERWVRLHKACNFRADITPLYTAQPVPVTPDAWISCS
                     ERMPEMGERQCYVLAADFKNNYPPNIPNTQVGVYGDWFNDGNPTWDDGDGEDLYLKEV
                     THWMQLPEPPQEVNRG"
     gene            837..1502
                     /locus_tag="ACHURS_003856"
     CDS             837..1502
                     /locus_tag="ACHURS_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239479.1"
                     /translation="MLLKMVNEARKLYGEPSVRNNKFIEKIEDELEGETYTKSVGRKN
                     GADIDVISMTIKQALRVAARESKAVRRTLVDKLESMQEAHIKSGKSASGLVEYRQART
                     LKMTVEAVTNLFDLMPNLAPEAKQTAAASIINPLVGFNAIPLPAIEEHYYSAGEVAEQ
                     LGVTANKIGRIANANNLKTEQYGKFFLDKSAHSSKQVETFRYNEAGVHKIEELIEGER
                     KAA"
     gene            1824..2447
                     /locus_tag="ACHURS_003857"
     CDS             1824..2447
                     /locus_tag="ACHURS_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HFR1239480.1"
                     /translation="MASQLIPVFNGTISNETTLLVNARDLHEFLEVRRDFSTWIKNRI
                     TEYGFAVDVDYILVHQNRGIKGRGGDRRSKDYHLTLDTAKETAMVERNEKGRQIRRYF
                     IECEKKLRSMQPAQQFTDEEIILLCYMQVQMENAQDICKRLYPILKELNSSYTSKLYD
                     IAFETFYTVTKNRDVLLREATRLDQASALFERAKPMLKSLRARQFEF"
     gene            2532..2750
                     /locus_tag="ACHURS_003858"
     CDS             2532..2750
                     /locus_tag="ACHURS_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFR1239481.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
     gene            2728..3798
                     /locus_tag="ACHURS_003859"
     CDS             2728..3798
                     /locus_tag="ACHURS_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1239482.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMS
                     KIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHI
                     TTKPVAATRAAKSEVRRSRLTADEYLKIYQTAESSPCWLRLAMELAVVTGQRVGDLCE
                     MKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTR
                     REPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHK
                     SDTMASQYRDDRGREWDKIEIK"
     gene            3933..5216
                     /locus_tag="ACHURS_003860"
     CDS             3933..5216
                     /locus_tag="ACHURS_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFR1239483.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNMYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(5450..>7611)
                     /locus_tag="ACHURS_003861"
     CDS             complement(5450..>7611)
                     /locus_tag="ACHURS_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFR1239484.1"
                     /translation="GTIAWSILSSHNTSGNMDKLKIKFDSLASHDITFVGIVQTAKAS
                     GMERFPLPYVLTNCHNSLCAVGGTINGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMRE
                     MMAGGGKMILGSDSHTRYGALGTMAVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKP
                     APYVGPQDVALAIIGAVFKNGYVKNKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSS
                     VWQTDEEVHNWLALHGRGQDYCQLNPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYE
                     IDTLNQNLTDILREIEIESERVAHGKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENV
                     IAAANALRGQSCGNDTFSLAVYPSSQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFG
                     AGDTPINNGLSIRHTTRNFPNREGSKPANGQMSAVALMDARSIAATAANGGYLTSASE
                     LDCWDNVPEYAFDVTPYKNRVYQGFVKGATQQPLIYGPNIKDWPELGALTDNIVLKVC
                     SKILDEVTTTDELIPSGETSSYRSNPIGLAEFTLSRRDPGYVGRSKATAELENQRLAG
                     NVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQRVIGGLAN
                     IAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIH
                     EDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
BASE COUNT         2093 a         1863 c         1896 g         1759 t
ORIGIN      
        1 cgctacgcga taagcaggcg ttacttgaag cctcagaaaa gcgcatagca gaactggagg
       61 cacagacagt taccgttaaa gaggttggag atgcttgata gttttgcgct cgttgcactg
      121 gttggcattg tagcgttgat ttgggtgaca tccgggaatc ggaggcataa gtgaacgaca
      181 aagaactgat taaagaaatc aaagagcgta tcagcagttt ggatgtgcga gacaatgttg
      241 aacgccgtgc ttatgaaatt gctctggcat cgctgacagc agagccggtg gcgtggaagg
      301 taaccttcac gcaaattgac cgtgaatata acacgttcac tggtatgtat tctgacaaag
      361 cagaagtcga acggtgggtg cggctgcata aagcatgtaa ttttcgggca gatataacac
      421 cgctttatac cgcccagcca gtgccggtaa ctccggatgc ctggataagc tgtagtgagc
      481 gaatgccaga aatgggagag cgacaatgct atgtgttagc agctgacttt aaaaacaact
      541 acccaccaaa catccccaac actcaggtcg gcgtatatgg cgactggttt aatgatggca
      601 atcccacttg ggatgacggt gatggcgaag acctgtatct caaagaggta acccactgga
      661 tgcagctacc agaaccgccg caggaggtta accgtggcta acctgcaact tgccgtcaaa
      721 ggtgaataac aatcctcgca ctcgcgggga tttcttttat acgatatagg ggaaaaggtg
      781 tggttaacaa tattgcaaat cactcttcgt ttccggctgt aacaatcgac agccagatgc
      841 tgttgaaaat ggtcaatgaa gctcgcaagt tatatggaga gccatcagta cgtaacaaca
      901 aattcatcga gaaaattgag gatgaattgg aaggcgagac ctacacaaaa agtgtaggtc
      961 ggaaaaacgg ggctgacatt gatgttatct ccatgactat caagcaggcg cttcgtgttg
     1021 ctgctcgcga atctaaagca gttcgccgaa cacttgtaga caaacttgaa agtatgcagg
     1081 aagcgcacat taaaagcggt aaatcagcga gtggacttgt tgagtatcgt caagcgcgaa
     1141 cactgaaaat gacggttgaa gctgttacca atctgttcga tttgatgcca aatcttgcgc
     1201 cggaagcaaa gcagactgct gcggcaagca taatcaaccc gctcgttggt tttaatgcaa
     1261 tacctcttcc ggcaatagaa gagcattact actcagcagg ggaggtcgca gagcagctcg
     1321 gagtaacggc aaacaagatt ggtcgcattg ctaacgcaaa caacctcaaa actgagcagt
     1381 acgggaagtt cttcctggat aaatctgcgc attccagcaa gcaggttgaa acatttcggt
     1441 acaacgaggc tggagtgcac aaaatagagg aactcatcga aggagagcga aaggctgcat
     1501 gattttgaca agatagtttt ccccaaatgt ggggaaaagc ccgaatggcg cggcttacag
     1561 caagataagg cctacatgat ttgacaacac cgcattaacg gggctatatt ccgcttcatg
     1621 gtgctgaaca caccttgcaa agcggaaacc gcacccgtca gtcatgcgga ttttttatgt
     1681 ccatttttca gatatggtcg ggtagcgcgt ataccgaaaa acagccgaaa ggttaaggat
     1741 acgggccgac tttgcacggt gttcaagtac ctgaccgccc tgctgaacac agggctatct
     1801 gaacaaatgc aaaggacata aaaatggcca gtcaactcat ccccgtattc aacggcacta
     1861 tatccaacga aacaactctt ctcgttaatg cccgtgattt acacgaattt ttggaagtac
     1921 gtcgtgactt ctctacatgg attaaaaatc gcataacaga atacggcttt gccgttgatg
     1981 tcgattacat tttggttcac caaaacaggg ggatcaaagg acgtggaggc gatcgccgta
     2041 gcaaagacta tcacctcacc ctcgatacag ccaaagaaac ggcgatggtc gagcgtaacg
     2101 aaaaaggccg ccagatacgc cgatacttca tcgagtgcga aaagaaactt cgcagcatgc
     2161 aaccagcgca gcaattcaca gacgaagaaa tcatcctcct ttgctatatg caggtacaga
     2221 tggagaatgc gcaggacatc tgcaaacgtc tgtacccgat attgaaggaa ctgaactcat
     2281 catacacgag caagctgtat gacatcgcgt ttgaaacttt ttacacggtg acgaaaaaca
     2341 gagatgtact gctaagggag gcgacacgac ttgaccaagc aagcgccctt ttcgaacggg
     2401 caaaaccaat gctgaaaagc cttcgggcga gacaattcga attttaatca tcaaaggagc
     2461 ttcggctcct tttttgttgg agaaaataaa ccaatactcg ctcccttgcg agtaattgcg
     2521 gagactttgc gatgtacttg acacttcagg agtggaacgc acgccagcga cgcccaagga
     2581 gccttgaaac agttcgtcga tgggtacgcg agtgcaggat attccctcct ccggttaagg
     2641 atggaagaga gtatttgttc cacgaatcag cggtaaaggt tgacttaaat cgaccagtaa
     2701 caggtagcct tttgaagagg atcagaaatg ggaagaaggc gaagtcatga gcgccgggat
     2761 ttacccccta acctttatat aagaaacaat ggatattact gctacaggga cccaaggacg
     2821 ggtaaagagt ttggattagg ccgagacagg cgaatcgcaa tcactgaagc tatacaggcc
     2881 aacattgagt tattttcagg acacaaacac aagcctctga cagcgagaat caacagtgat
     2941 aattctgtta cgttacattc atggcttgat cgctacgaaa aaatcctcgc cagcagagga
     3001 atcaagcaga agacactcat aaattacatg agcaaaatta aagcaataag gagggggctg
     3061 cctgatgctc cacttgaaga catcaccaca aaagaaattg cggcaatgct caatggatac
     3121 atagacgagg gcaaggcggc atcagccaag ttaatcagat caacactgag cgatgcattc
     3181 cgagaggcaa tagctgaagg ccatataaca acaaaaccgg tcgctgccac tcgcgcagca
     3241 aaatcagagg taaggagatc aagacttacg gctgacgaat acctgaaaat ttatcaaaca
     3301 gcagaatcat caccatgttg gctcagactt gcaatggaac tggctgttgt taccgggcaa
     3361 cgagttggtg atttatgcga aatgaagtgg tctgatatcg tagatggata tctttatgtc
     3421 gagcaaagca aaacaggcgt aaaaattgcc atccctacaa cattgcatgt tgatgctctc
     3481 gggatatcaa tgaaggaaac acttgataaa tgcaaagaga ttcttggcgg agaaaccata
     3541 attgcatcta ctcgtcgtga accgctttca tccggcacag tatcaaggta ttttatgcgc
     3601 gcacgaaaag catcaggtct ttccttcgaa ggggatccac ctacctttca cgagttgcgc
     3661 agtttgtctg caagactcta tgagaagcag ataagcgata agtttgctca acatcttctc
     3721 gggcacaagt cggacaccat ggcatcacag tatcgtgatg acagaggcag ggagtgggac
     3781 aaaattgaaa tcaaataatg attttatttt gactgataat gacctgttcg ttgcaacaaa
     3841 ttgataagcg atgctttttt ataatgccaa cttagtataa aaaagcaggc ttcaacggat
     3901 tcatttttct atttcatagc ccggagcaac ctgtgaacac attttcagtt tcccgtctgg
     3961 cgctggcatt ggcttttggc gtgacgctga ccgcctgtag ctcaaccccg cccgatcaac
     4021 gtccttctga tcaaaccgcg cctggtacct cttctcgccc gattctgtcg gcaaaagaag
     4081 cgcagaattt cgatgctcaa cactattttg catccctgac accaggtgct gcagcgtgga
     4141 atccttcccc gattaccctg cctgcgcaac ctgactttgt tgtcggcccg gcgggcactc
     4201 aaggtgtaac gcataccacg attcaggcgg cggtagatgc ggcaattatc aagcgtacca
     4261 acaagcgcca gtatattgcc gtgatgcctg gtgagtatca gggaacggta tatgtccctg
     4321 ccgctccggg tggaattact ctgtacggta caggtgaaaa accgattgat gtgaagattg
     4381 ggctttccct tgatggtggc atgagccctg ccgactggcg tcacgacgtc aacccgcgcg
     4441 gcaaatatat gccaggtaaa ccagcgtggt atatgtacga tagctgccag agcaaacgca
     4501 gcgacagtat cggtgttctc tgctctgcgg tcttctggtc acaaaacaat ggcctgcaac
     4561 tgcaaaatct gaccatcgaa aacacgctgg gcgatagcgt agatgcaggt aaccatccgg
     4621 cggtggcact gcgtactgat ggtgaccagg tacagattaa caacgttaac attctcggtc
     4681 gtcagaacac cttctttgtc accaacagcg gtgtgcagaa ccgtctggaa acgaatcgtc
     4741 agccgcgtac gctggtgacc aacagctaca ttgaagggga tgtggatatc gtttctggtc
     4801 gcggcgcagt ggtgttcgat aacaccgaat tccgcgtggt gaactcacgt actcagcaag
     4861 aagcgtatgt gtttgcaccg gctacgctgt ccaacatgta ctacggtttc ctcgccgtaa
     4921 acagccgttt caatgctttc ggtgatggtg tggcgcaact gggccgctcg ctggatgttg
     4981 atgccaatac caacggtcag gtggtgatcc gtgatagcgc catcaacgaa ggttttaaca
     5041 cggctaaacc gtgggccgat gcggtgatct ctaatcgtcc gtttgcgggt aataccggca
     5101 gcgtagatga taacgacgaa atacagcgca atctgaatga cactaactac aaccgcatgt
     5161 gggaatacaa taaccgcggc gtgggtagta aagtggttgc agaggcgaag aagtaagagc
     5221 aattaactat ttgccggatg cggcgtaaac gccttatccg gcctacggtt cgatgcgatt
     5281 tgtaggtcgg ataagatgcg caagcatcgc atccgacaat aagtgccgga tgctgcgtaa
     5341 atgccttatc tggcctacag attcgatgcg attcgtaggt cggataagat gcgcaagcat
     5401 cgcatccgac aataagtgcc gaatgcgacc tacattcaca tggcgctttt tacatctgac
     5461 ggtttttatt gaagttaatc aaactacccg ccttgataat ctcgcgctct tcagcagtca
     5521 gactttccat atagagcgta atttccgtta ccggcgcatc ttcatggatc acataacctt
     5581 taaacgtcgt acccggatta tccagcgccg ctttaatgcc agggatgtaa atgtaatccc
     5641 ccacttcaaa ggttggcact tccgccatct gcagaggtaa catcccccag ttgatgacgt
     5701 tagaacggta gcgtttagtc gcgtactcct cggcaatatt cgccagaccg ccaatcacac
     5761 gctggcagct cgccgcctgt tcacgcgcag aaccatcgcc tggtttcacc gcatagacca
     5821 tgctgccaat ttcagtttgc agcggatcaa tatgctcctg accagcaatc tgcttaatgc
     5881 gcgcaaacac ctctgtcagc tcgctgacat tccccgccag acgctgattt tccagctcag
     5941 cagtcgcttt acttctgcca acataaccgg gatcacggcg agacagggta aactccgcca
     6001 gaccaatcgg atttgaacga taagaagagg tttcaccgga aggaatcagt tcgtcggtgg
     6061 tggtcacttc gtcgaggatc ttcgagcaca ctttcaggac gatattgtca gtcagcgcac
     6121 ccaattccgg ccagtcttta atgttcggcc cgtaaatcag cggttgctga gttgcccctt
     6181 tcacaaagcc ctgataaaca cggtttttat acggcgttac atcgaaggcg tactccggca
     6241 cgttgtccca gcaatcaagt tcgctggcag aggttaaata gccaccgttt gccgcagtcg
     6301 cagcgataga acgagcgtcc atcaacgcca ccgctgacat ctgcccatta gctggcttag
     6361 agccttcgcg gttcgggaag ttacgcgtgg tgtggcgaat actcaaaccg ttgttgattg
     6421 gcgtatcgcc cgcgccaaag catgggccgc agaacgcggt tctgatgatt gcgcctgcgc
     6481 caatcaaatc tgctaccaca ccttttttgg cgagatccat aaacaccggc tgtgatgacg
     6541 ggtaaactgc cagcgagaag gtgtcattgc cacaggattg accgcgcagt gcattcgccg
     6601 ccgcgatgac gttttcgtag ttaccgccag aacagcccgc gataatcccc tgctgcactt
     6661 tcaggcgacc attttccact ttatccagca gcgagagttt ggctttaccg tgcgccacgc
     6721 gttcggactc aatttcaatc tcacgcagaa tgtcggtcag gttctggttc agtgtgtcga
     6781 tttcatacac gttgctcggg tggaacggca gcgcaatcat tggtttgatg gcgcttaaat
     6841 caacgctgat gcagccatcg tagtacgcca tcggttgagg gttaagctgg cagtaatcct
     6901 ggccgcgacc gtgcagcgcc agccagttat gcacttcttc atcagtttgc cagacggagc
     6961 ttaaacaggt ggtttccgtg gtcatcacgt caacgctgtt acggaaatcg gtagagagcg
     7021 cgctaacgcc cggtccaacg aactccatga ctttgttttt gacgtaaccg tttttgaaca
     7081 ccgcgccaat gatagccagc gccacatcct gtggccccac atacggcgca ggttttccgg
     7141 tcagatgcac cgcaaccacg cccggatagt cgatatccca ggtgtcatta agcagctgtt
     7201 ttaccaactc accgccaccc tcaccgactg ccattgtccc taatgcaccg taacgggtgt
     7261 ggctgtctga cccgaggatc attttgcccc cgcctgccat catctcacgc atatattgat
     7321 ggatgaccgc aatatgcgga ggcacaaaaa taccgccata acgctgagct gccgataaac
     7381 caaaaacatg gtcatcaccg ttaatggtgc cgccgacggc gcagagtgag ttatggcagt
     7441 tggtcagcac atacggcagc gggaaacgtt ccattccgga cgctttagcg gtctgtacaa
     7501 taccaacaaa ggtaatatcg tgagaggcta atgaatcaaa cttaatttta agtttatcca
     7561 tatttccgga cgtattatga gaagagagaa tagaccaggc aatagtgcct t
//
LOCUS       DAZPDU010000198         7536 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZPDU010000198 DAZPDU010000000
VERSION     DAZPDU010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7536
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.200"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(7..383)
                     /gene="rnpB"
                     /locus_tag="ACHURS_003862"
     ncRNA           complement(7..383)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHURS_003862"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(416..1561)
                     /gene="garK"
                     /locus_tag="ACHURS_003863"
     CDS             complement(416..1561)
                     /gene="garK"
                     /locus_tag="ACHURS_003863"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFR1239485.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESSATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(1658..2548)
                     /gene="garR"
                     /locus_tag="ACHURS_003864"
     CDS             complement(1658..2548)
                     /gene="garR"
                     /locus_tag="ACHURS_003864"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFR1239486.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLLLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2578..3348)
                     /gene="garL"
                     /locus_tag="ACHURS_003865"
     CDS             complement(2578..3348)
                     /gene="garL"
                     /locus_tag="ACHURS_003865"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFR1239487.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3364..4698)
                     /gene="garP"
                     /locus_tag="ACHURS_003866"
     CDS             complement(3364..4698)
                     /gene="garP"
                     /locus_tag="ACHURS_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFR1239488.1"
                     /translation="MILDTVDVKKKGVHTSYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGGIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     VSTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5073..6644
                     /gene="garD"
                     /locus_tag="ACHURS_003867"
     CDS             5073..6644
                     /gene="garD"
                     /locus_tag="ACHURS_003867"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFR1239489.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGVQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEESIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            6793..7205
                     /locus_tag="ACHURS_003868"
                     /pseudo
     CDS             6793..7205
                     /locus_tag="ACHURS_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312034.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PrlF family
                     antitoxin"
     gene            7205..>7536
                     /gene="yhaV"
                     /locus_tag="ACHURS_003869"
     CDS             7205..>7536
                     /gene="yhaV"
                     /locus_tag="ACHURS_003869"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFR1239490.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGW"
BASE COUNT         1900 a         2021 c         1835 g         1780 t
ORIGIN      
        1 taaatcaggt gaaactgacc gataagccgg gttctgtcgt ggacagtcat tcatctaggc
       61 cagcaatcgc tcactggctc aagcagccta cccgggttca gtacgggccg taccttatga
      121 acccctattt ggccttgctc cgggtggagt ttaccgtgcc acggactgtt accagccgcg
      181 cggtgcgctc ttaccgcacc ctttcaccct tacctgatcc cgcttgcgcg ggccatcggc
      241 ggtttgctct ctgttgcact ggtcgtgggt ttccccccca ggcgttacct ggcaccctgc
      301 cctatggagc ccggactttc ctcccctccg cccgtctccc ccgaagagga cgacgacgaa
      361 gcggcgactg tctggtcagc ttcggcgcgc agtatagagg gtttgcgcgc ccttgtcacc
      421 ccgcgttgcg cattccaatc gccagtgtcg cggcgatatt acgtgaagca cggcagatat
      481 tgtcataagc cccgcggaat gcttcgtcca acgtacctat gctggtcaat acgctgaaga
      541 ccgcatcgat gccatgctga tgtacaacgc caacatcatc ggtcaggcta cccgcaatgc
      601 caatcaccgg tttatggtac ttcttcgcca cgtttgcaac gccaatcggt accttgccgt
      661 gaatactctg gctgtcaata cgcccttcac cggtgatcac cagcgtacaa tcgtgaatat
      721 gttcttccag attcagcgcc gtagtgacga tttcaatacc acttttcagt tccgcaccaa
      781 gaaacgccat cagcgccgcg cccataccgc ccgcagctcc tgcgccgggg acatctttca
      841 catcaacatg cagcgctttt ttaatgacat cggcatagtg agagaggtta ttgtccagct
      901 caacaatcat cgcttcactg gctccctttt gtgggccaaa gatgcgcgat gcgccgttat
      961 cgcccaccag cggattggtg acatcacaag cgacgcgaat gacgcaatct tttaagcgcg
     1021 gatcgaggcc ggaaatatca atatcattca gagtattaag actaccgccg ccaaaaccaa
     1081 tttcattgcc gttggcgtcg cataatttcg cccccagcgc ctgtaccatg cctgcgccgc
     1141 catcatttgt agcgctgccg ccaatgccga taataatgtt tgttgcactg ctctccagcg
     1201 cctgcaggat taactcgcct gtgccgcgtg aagtggttac gagtggatcg cgtttttccg
     1261 caggtaccag ctccagccca ctggccgccg ccatttcaat aaacgcggtt ttgccatcgc
     1321 cggagatccc ccaactggca ttcactttct cgcccagcgg ccctgtaacc caggcgtgac
     1381 gttcagcccc ctgggtggct gcaatcatcg cttccaccgt tccttcacca ccgtcggcaa
     1441 ccggaacaga aacatactgt gcatcaggaa aaatttcccg aaatcctttt tctatcgcct
     1501 gcgcaacctc gctggcagat aaactttctt tataagagtc tggggcgatt acgattttca
     1561 tacctatgcc tgttaccaca tgacgccgga gggcgtttct cttattcggc ctagattcca
     1621 ggcccggatt gcaatacacc atccgggcac gacgtcatta acgagtaact tcgactttcg
     1681 ccagtttttc gtagtagcac gccagggcgc tatgatccgc cgttcctaaa ccatctgcac
     1741 gcagtgcctg catcatctcc ataaccgcag ctgtgagcag cagttgtgcg ccgacgccgt
     1801 gagaagtatc cagcgcattc gccagatcct taatatgcag atcaatgcgg aagcccggct
     1861 tgaagttgcg gtccatcacc atcggcgctt tggcatccag cacggtactg cccgccagcc
     1921 caccgcgaat tgcctgataa accaggtccg ggttaacgcc cgctttggtt gccagcgtta
     1981 acgcttctga catcgcagca atattcagcg ccacaatgac ctgatttgcc agtttggtga
     2041 cgttacctgc accgatttcc ccggtatgca ccacggaacc cgccatcgct ttcatcaaat
     2101 catagtattt gtcgaaaata gccttgtcgc cgcccaccat cactgacagc gtaccgtcga
     2161 tggctttcgg ttcaccgccg ctcaccggag catccagcat atcaatgcct ttcgctttca
     2221 gcgcttcgct gatttcacgg cttgccagcg gtgcgataga actcatatcg atcaataccg
     2281 tacctggctt cgcgccttca ataatgccat tctcacccag cgccacctct ttcacatgag
     2341 gggagtttgg cagcatggtt atgatgacgt cgcactgttc agcgatcgct ttagccgtag
     2401 acgctgtttc tgcacctgca gcaatcacgt cagcaatagc ttctgggtta cggtcagcaa
     2461 ccaccagcga gtaacctgct ttcagaaggt ttttactcat tggtttaccc ataatcccca
     2521 ggccaataaa accaactttc atagtcatat caaccatctc tcttgttgcg gtggtggtta
     2581 ttttttaaag gtatcagcca gtttctgagt ggcagagcgg aagacgccaa gatcgctgcc
     2641 gacagccaca aacgtcgcgc cccattccag ataacgacgc gcatcggctt cgaccggcgc
     2701 gagaataccg ctgggtttgc cgtgcgcgct ggcacggtta aaaatatgct gaattgcttt
     2761 ttgtacatcc ggatgcgagg cattgccgag atggcccaat gccgcagcca gatcgctggg
     2821 gccgacgaag ataccgtcta caccttcggt agcggcaata gcatcgacgt tatctacgcc
     2881 ctgctgactt tctatctgga ccagaatagt aatgttcttg ttcgactgag cgaaataatc
     2941 cgccacggtg ccaaacatat tggcgcggtg agaaacggag acgccgcgaa tgccttccgg
     3001 cgggtaacga gttgatgcca ccgcctgctc tgcttcctct tttgtttcta caaaaggaat
     3061 caggaagtta tagaaaccga tatccagaag acgcttaata attaccggct cgttggtcgg
     3121 cactcgcact actggcgcgc tggcgctgcc tttcagggcc attaactgcg gaataaacgt
     3181 ggaaatatcg tttggcgcat gttcgccatc cagcaccagc cagtcaaacc cagccaaacc
     3241 aagaacttca gtgctaatcg ggttagagag tgctgaccag caaccaattt gtacctgttt
     3301 cgcagccagt gcggctttga atttattcgg gaaaacatcg ttattcatcg cttatacctt
     3361 tgcttatttc tgcaattcca tacgtttaat gtcgccaact acgaagaggt agcagaccat
     3421 cgccatcagc gctgaacatc ccacgaaaac cagtgctgca ttgaaggagt gcagttcact
     3481 taccaggtag ccaatcacca gtggagtgac aatggaggca acattgccaa agacgttaaa
     3541 gacgccgcca cagaggccaa caatctcttt cggcgcggtg tcagaaatca ccggccagcc
     3601 cagagcacca aatcctttgc caaaaaaagc cagcgccatc agcatgacca ccagcgtggt
     3661 gttgttggtg tagttacata agatgatggt ggaaaccagc aacattccca gcacaatcgg
     3721 tagcttacgt gccagggtca gggataaacc gcgtttgatc agataatccg agaagacacc
     3781 tcccagcacg ccgcccgcaa aaccacacag tgccggaatc gaggcgacca gacccacttt
     3841 cagaatcgac atgccttttt cctgcaccag ataaatcggg aaccaggtga ggaagaacca
     3901 ggtgatggtg ttgataaaat attgtccgaa aaatacgccc aacatcatgc ggttagagag
     3961 caattgcttg atgtaatgca gtttgggacc gcttgctgcc gcgctgcccg gctttttgtg
     4021 gtccatatcg accaccgcgc cattttcaga gataaacttc agctcttccg cagacatacg
     4081 tgggtgatct gtcgggttat gaatcaactt gatccacagc gccgtcagca caaaaccaat
     4141 cccccccata acggtgaaga cgtgctccca gccccaagcg aaagtcagcc agccaagcag
     4201 cggcgaaaag agcgccagcg agaaatattg cgccgagtta aagatggcgg aggcagtacc
     4261 acgttctttc gtcgggaacc aggcggcgac aatacgggcg ttcgccggga atgatggcgc
     4321 ttccgagaac cccagcataa agcgcataaa gaacatggag atccctgccc aggccagcgg
     4381 gaacatatca acaaagcctt gcaggaaggt gaacagcgac cagaaaaaga ggctgtaggt
     4441 gtaaactttt ttcgagccaa acttatcaag caaccagccg ccggggattt gcatcagcaa
     4501 gtaggcccag ccaaaagcag agaagatgta acccatcgaa accgcactta actgcaactc
     4561 ttttgccact tcggtaccag caatagacag cgttgcacga tcggcgtagt taacggcggt
     4621 aacaataaaa ataatcagta atattaaata gctggtatgc acgcctttct tttttacgtc
     4681 aacggtgtcc agaatcattt tatttacctc gggtacttat gctgattttt attattatgg
     4741 ggaaggtgtt atttatgagt tttatttatg ccgttacgac aatgaactcg ggaattagta
     4801 taagcagcgg gagaataata atcattgtgc aaatgctaat ttaatcagta ctatttaaat
     4861 attattttga gcatatgcac ataaggttgc gcgctaaagc acagatttgc gctttacctt
     4921 accgggcggc actgcaatcc ctgaaatgat tgacattgat cacatttctg cgtttaaact
     4981 cctgacattc ttatttcacc caatgaagtc atttattttt aaatgagacc agatcctcat
     5041 tttaataacc cctggctgga gaatattgca caatggccaa catcgaaatc agacaagaaa
     5101 cgccaactgc gttttatata aaagttcacg acacagataa tgtggcaatt attgttaatg
     5161 ataatggcct gaaagcagga acgcgttttc cggatgggct ggaattaatt gaacatattc
     5221 cccaggggca taaagtcgca ttgctggaca ttccagctaa tggtgaaatt attcgttatg
     5281 gcgaagtgat tggttacgcc gtgcgcgcaa tcccacgcgg aagctggatc gacgaatcaa
     5341 tggttgtact accggaagcg ccgccgttac acacgctgcc actggcaacc aaagtcccgg
     5401 cccccttacc gccgctggaa ggatacacct ttgagggcta tcgcaatgcc gatggcagcg
     5461 tgggcaccaa aaacctgctc ggcatcacca ccagcgtcca ctgtgtggca ggcgtggtgg
     5521 actatgtagt aaaaatcatt gaacgcgatc tgctaccgaa atacccgaac gtcgatggcg
     5581 tggtggggct gaatcatttg tacggctgtg gcgtggcgat taacgcaccg gcggcagttg
     5641 tgcctattcg taccatccac aatatttcgc tgaaccctaa ctttggcggc gaagtaatgg
     5701 tgattggtct gggttgtgaa aagttgcagc ctgagcgcct gctggtcggc acggatgatg
     5761 tgcaagctat tccagtagaa agcgccagca ttgtcagttt gcaggatgaa aagcatgtcg
     5821 gctttcagtc catggtcgag gatattttgc aggtcgccga acgccatcta caaaaactga
     5881 atcaacggca gcgagaaacc tgcccggctt cagaactggt cgttggtgtg cagtgcggtg
     5941 gcagcgatgc gttttctggt gtaacggcaa acccggcggt tggctatgcg tctgatctac
     6001 tggtgcgctg cggcgcaacg gtgatgtttt cagaagtaac ggaagtgcgt gacgcgatcc
     6061 atctgctgac accacgcgca gtgaacgaag aggtcggcaa acggctgctg gaggagatgg
     6121 agtggtacga taactatctc aatatgggaa aaaccgaccg cagcgccaac ccttcgccgg
     6181 gcaacaagaa aggcggtctg gcaaatgtgg tggagaaggc actcggctcc attgctaaat
     6241 cgggtaaaag tgcaattgtt gaagtgctgt cgcccggtca acgcccgact aaacgcggat
     6301 taatttacgc cgcgacgcca gccagcgatt ttgtctgtgg cacgcaacag gtggcttcgg
     6361 gtatcaccgt acaagtgttt acgaccggcc gtggtacgcc gtacggcctg atggcggtac
     6421 ccgtaattaa aatggcaacc cgcaccgagc tggcgaaccg ctggtttgat ttaatggata
     6481 ttaacgcggg cactatcgct accggcgaag agagcattga agaggtgggc tggaagttgt
     6541 tccactttat tctcgacgtc gccagcggga agaagaaaac cttctcggat caatgggggc
     6601 tgcataacca gctggcggtg tttaacccgg caccggtgac ctgatttctt ttggaacgtc
     6661 cctcgcaaaa catggcctta gtgccatgtt tttattgttt aaagccccca cgtccattaa
     6721 taatgcattt gcattacctt agttcaagct tataatttga gcagaaaaca ggctgtaaaa
     6781 ggacaatgaa tcatgcccgc taatgctcgc tctcacgctg tactgaccac tgaatcaaag
     6841 gtcacgatac gcggacaaac aactatcccc gcgccagtgc gtgaggcctt aaaactgaag
     6901 ccaggccagg acagcattca ttacgaaatt ctgcctggtg ggcaagtatt tatgtgccga
     6961 ctgggagatg aacaggagga tcatactatg aatgcatttt tgcgttttct ggatacagat
     7021 atccagaaca acccgcaaaa aactcgtcca ttcaacattc aacattcaac attcaacatt
     7081 caacattcaa cattcaacat tcaacattca acattcaaca ttcaacattc aacattcaac
     7141 aaggaaagaa acttgtcgct ggcatggacg tcaacattga tgatgagatt ggcgacgacg
     7201 aataatggat tttccacaaa gggttaatgg ttgggcgcta tatgctcatc cctgttttca
     7261 ggaaacctac gacgctttag ttgccgaagt cgagacatta aagggaaaag atcctgaaaa
     7321 ttatcagaga aaagccgcca caaagttatt ggcggtagtc cataaagtga ttgaggagca
     7381 tatcacggtc aatccatcat caccggcatt ccgtcatggc aagtcgttag gctctgggaa
     7441 aaataaagac tggtcacggg taaaatttgg tgcaggtcgt tatcgtctct tctttcgtta
     7501 tagtgaaaaa gagaaagtca tcattctggg atggat
//
LOCUS       DAZPDU010000199         7491 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZPDU010000199 DAZPDU010000000
VERSION     DAZPDU010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7491
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.201"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(16..522)
                     /gene="mug"
                     /locus_tag="ACHURS_003870"
     CDS             complement(16..522)
                     /gene="mug"
                     /locus_tag="ACHURS_003870"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFR1239491.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            647..722
                     /locus_tag="ACHURS_003871"
     tRNA            647..722
                     /locus_tag="ACHURS_003871"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:680..682,aa:Ile,seq:cat)
     gene            complement(776..1540)
                     /gene="nfeF"
                     /locus_tag="ACHURS_003872"
                     /gene_synonym="yqjH"
     CDS             complement(776..1540)
                     /gene="nfeF"
                     /locus_tag="ACHURS_003872"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFR1239492.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            1828..2451
                     /gene="nfeR"
                     /locus_tag="ACHURS_003873"
                     /gene_synonym="yqjI"
     CDS             1828..2451
                     /gene="nfeR"
                     /locus_tag="ACHURS_003873"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFR1239493.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDHAAFDITQLD"
     gene            complement(2558..4078)
                     /gene="aer"
                     /locus_tag="ACHURS_003874"
     CDS             complement(2558..4078)
                     /gene="aer"
                     /locus_tag="ACHURS_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFR1239494.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            4496..5875
                     /gene="ygjG"
                     /locus_tag="ACHURS_003875"
     CDS             4496..5875
                     /gene="ygjG"
                     /locus_tag="ACHURS_003875"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFR1239495.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQADQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(5917..6249)
                     /locus_tag="ACHURS_003876"
     CDS             complement(5917..6249)
                     /locus_tag="ACHURS_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFR1239496.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            6468..7451
                     /gene="ebgR"
                     /locus_tag="ACHURS_003877"
     CDS             6468..7451
                     /gene="ebgR"
                     /locus_tag="ACHURS_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFR1239497.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLFSVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
BASE COUNT         1823 a         2031 c         1869 g         1768 t
ORIGIN      
        1 tttcggtggc gttttttatc gcccacgcac taccagcgcc tggtccagct cgcgatacgc
       61 ttcaaccagt ttctctagtg aaacgcgact taaaccgctg ggatttggca gcacccaaat
      121 ctgcgtcgaa ccaatggtga gcgtttgttt cccccactgt gcaccgcgct ggctgaatcc
      181 ctgttcatat gcttgtttgc ccagaatcgc caacgcctgc ggctgataat cttcaatttt
      241 ttcaatcagc ttacgcccac ctgcgtgcag ctcctgcttt gaaacttcat tggcttgcac
      301 cgttggacgg tctaccagtt tggtgacgcc acaacgataa tccagcagat gctgtgcctc
      361 ctgcggcttc aactgacggt cggtaaaccc ggcctgatat atcaccttcc agaagcgatt
      421 tgctggatga gcaaagggaa aaccagtccc ggcggatgaa agcccagggt tgataccgca
      481 aaacacgacc cgtaaccctg gagccaaaat atcctcaacc atatttactc ctttctaaca
      541 tcaccaggaa aagtataacg gattgaaaat acattgttta taaaaacagc aggcgcacgg
      601 taatggctgg attgcgacac ggagttactt tataatccgc taccatggcc ccttagctca
      661 gtggttagag caggcgactc ataatcgctt ggtcgctggt tcaagtccag caggggccac
      721 cagatatagc aaaggctgac gagaaatcgt cagccttttt ctttttatat atcagttact
      781 ttgcgtgcca gtaagccgct gcacgtaccc gctgtgggtc atactgttcc gcttcaaagc
      841 ggcggcttaa attcttaacg actttacctt cgccggttat ccagatgaag taatcatcgg
      901 cagggatttg catctgcgcc agacgcgcat ctaccgcctg ctcatcatgt gccagccatt
      961 cgatattaaa accatcaagg tgcgcgagat aatcctgaca ggcgttatcc cgcacgctaa
     1021 ccagcgcact aacttgcggt ttaacggcaa gtttgctcaa cgtttccaag cggcggcgca
     1081 gtgcgggcat tccggattca tcgcagacat acagctgata cgcgtaatct tccggcacca
     1141 ccagcgaacc gcgcggacct gcaaccgtaa gtttatcgcc cggttgcgcc tgcatcgccc
     1201 agccgctggc gaccccaccg tcgtgaataa agaaatcgat cgccagttca tggcgtagtt
     1261 cgtcatacag cggcgtatag tcacgcgacg gtgggcgtgg tccttccggc cagacgatgc
     1321 cctcttccgt taccgttggc ggcacaaagt gagcgtcagg ttgaggaaag aagagtttgc
     1381 tgtgatcgtc aaagccgcgc gatgtaaaac cgtccagcgc ctcgccgccg aggacaatgc
     1441 gctgaaaacc ggcgctgatg cgctcaacgc gtaacacagt cagttcacgg aagcgcagat
     1501 cattgcgaac gcgctgcggg tagcgggggg agttattcat tgttatcgcc ttcgtgatgg
     1561 taatcagata tatctaaata attttcgtaa atgataatga ttgttaatca tgataaatgc
     1621 aagcgatttg tagaactgat atgtctatag tctgataaga cgaaccgcct cttctcaggc
     1681 atcattactc aacgccggat gcggcgtgaa cgccttatcc ggcctacgtg tgagatgagt
     1741 ccacatttaa aaatcatttt tattcttgca agagtgctca tatcagatat aaattagata
     1801 tatctaatca agcaaaagga ggctgatatg agccatcatc acgaagggtg ttgtaaacat
     1861 gaaggccagc cacgccatga gggctgctgc aaaggtgaga agtcagaaca cgagcactgc
     1921 ggacacggtc accagcatga acacggtcaa tgctgcggtg gtcgccacgg tcgcggcggc
     1981 ggtcgtcggc aacgtttctt tggtcacggt gaattgcgtc tggtgattct ggatattctc
     2041 tcgcgcgatg acagccacgg ttacgaattg attaaagcga ttgagaatct aacccagggg
     2101 aattacaccc caagcccggg cgtcatctac ccaacgctgg attttctgca ggagcagtcg
     2161 ctgattacca tccgcgaaga ggaaggtggt aagaagcaga ttgcgctgac cgaacaaggc
     2221 gcgcagtggc tggaagaaaa ccgcgaacag gtggagatga ttgaagaacg catcaaagcg
     2281 cgttgcgttg gtgctgcgct gcgccagaac ccgcaaatga agcgggcgct ggataatttt
     2341 aaagcggtgc tggatttacg cgtcaaccag agcgatatca ctgatgcaca aataaaaaag
     2401 atcattgcgg tgatcgacca cgccgctttt gatattaccc aactggatta atcaccgatt
     2461 gccggatgcg gcgtacccta cacaccccgc aggcctgata agatgcgcca gcatcgcatc
     2521 aggcattgtg ctccaaccgc cggatccggc ataccgatta atgcagcacc gtcaccgcgt
     2581 cttccagtcg gctggcgcgg tgtttcacca tcgccgacac ctgcgcactc tcttccacca
     2641 gctcagcatt tttctgggtg atcaggttaa gctcatccac tgcacgggtc aggctggaaa
     2701 gcccatcggc ctgttccagc gttgaatggc taatctgggc gatcaactgg gtgacgtttt
     2761 tcacctgtgc cacaatatct tccatcgtcc gtccggcggc gtgtacctgc tgcgaaccgg
     2821 attgcacctt atcagcactg gcatcaatca gcttgcgaat atcgttggca gcattagcgc
     2881 tgcggctggc taaatgacgc acttcccctg ccaccactgc aaaacctttg ccctgttcgc
     2941 cggcacgcgc cgcttccacc gccgcattca gggccagaat attggtctga aacgcaatat
     3001 cgttaatcag cgaagtaatg gtgccaatgc gctgggtact gtcggcgata tcgtccattg
     3061 tcttgatcac cgtggtcatc gcctccccac cctgcactgc cgcattactg gcggtgatag
     3121 aaagtttatc ggcagccgac gccgtggcag agttctgttt caccgacgcc gccatttggt
     3181 tcatggtcgc caccgtttgc tgaacgttat caactgtctg ctgggtatgt tcgttcagtt
     3241 catcggtgcc tttcgccagc gtctcactgc catttctgac actggacacc tggcttgaga
     3301 catcgttaat tagccaacgg cacatcaggc caagctgccc taccgcgcgt aatgtcagcc
     3361 ccagctcatc gctgcgattc aggtgctcaa cactattacg ctctccggtc gccaccttca
     3421 gtgcctgacg ggcgacattt tctatcgggc gcacaatctg ccattcaaaa caagcgcttg
     3481 ccaacaatac cactaacaca cagaggaaat acgtcaccac cggggcagca acaaaccaaa
     3541 gcatggccgc cagcaagata aacatcaggg tcatcactcc acgcgcccgc cagcgaagcg
     3601 gtaatgaagg cagtttaccc agccagcctt tacgcaccac caggccttta tgaatacgct
     3661 tactggtacg tccggcgttc agcgctttgt acagcggctc caccgccgca atctcttcat
     3721 ccgtcgcccg ggtacgaatc gacatatagc cactgatttt tccctcgcgc accatcggta
     3781 ccgcattggc ccgcacccaa taatggtcgc catttttgcg gcgatttttc acgatgccgc
     3841 tccagggctc cccttttttc agggtgaacc acatatccgc aaacgccgct tttggcatat
     3901 ccgggtgacg caccatgttg tgcggctgcc cttgtaactc ttgcaaggta aagccgctca
     3961 cctgcacaaa agtgtcatta gcatgagtga tatagctttg cagatcggta gtggacatca
     4021 gagtggtatc gtccgccagc ggggtatttt gctgggtgac atacggatga gaagacatgt
     4081 tcgcgtcctg tgcaggttat atggttgtta acttcttgtc agagtttatg tcggccccgc
     4141 tgcggttatc tttaaccgat taatttgatt tagatcgcaa tttgcgatta aagcacaaat
     4201 ctaattcttt gatttaaaat actttcactc tgttactata cgaaaacgtt aattatcttg
     4261 cccaaaaatc aggcaattat tgccctgaaa acgtgcattt gcgcagcaat catcaaatcc
     4321 atacccgaca aaaaccgtgc aaaataacaa caaatgttaa cagatagcat taaatattgc
     4381 acaaatgata accgaatttg tgtttatccc gattttcgcg atcgcagccg gagtggcgca
     4441 atccctgcaa tacttaaatc ggtatcatgt gatacgcgag cctccggagc atattttgaa
     4501 caggttacct tcgagcgcat cggctttagc gtgcagcgcc cacgccctga atctcattga
     4561 gaagcgaacg ctggatcatg aggagatgaa agcacttaac cgagaggtga ttgaatactt
     4621 caaagagcat gtcaatccgg ggtttttaga gtatcgcaaa tctgttaccg ccggcgggga
     4681 ttacggagcc gtagagtggc aagcgggaag tttaaatacg cttgtcgaca cccagggaca
     4741 ggagtttatc gactgcctgg gaggttttgg aattttcaac gtggggcacc gtaatccagt
     4801 tgtggtttcc gccgtacaga atcaacttgc gaaacaaccg ctgcacagcc aggagctgct
     4861 cgatccgtta cgggcgatgt tggcgaaaac ccttgctgcg ctaacgcccg gtaaactgaa
     4921 atacagcttc ttctgtaata gcggcaccga gtccgttgaa gcagcgctga agctggcgaa
     4981 agcttaccag tcaccgcgcg gcaagtttac ttttattgcc accagcggcg cgttccacgg
     5041 taaatcactt ggcgcgctgt cggccacggc gaaatcgacc ttccgcaaac cgtttatgcc
     5101 gttactgccg ggcttccgtc atgtgccgtt tggcaatatc gaagccatgc gcacggctct
     5161 taacgagtgc aaaaaaaccg gtgatgatgt ggctgcggtg atcctcgaac cgattcaggg
     5221 tgaaggtggc gtaattctgc cgccgccggg ctatctcacc gccgtacgta agctatgcga
     5281 tgagttcggc gcactgatga tcctcgatga agtacaaacg ggcatggggc gcacgggcaa
     5341 gatgttcgcc tgcgagcatg agaacgtaca gccggatatc ctctgccttg ccaaagcgct
     5401 cggcggcggc gtgatgccga ttggcgcgac catcgccact gaagaggtgt tttcagttct
     5461 gttcgacaac ccattcctgc ataccaccac ctttggcggc aacccgctgg cctgtgcggc
     5521 ggcgctggcg accatcaatg tgttgctgga gcagaactta ccggctcagg ctgatcaaaa
     5581 aggcgatatg ttgctggacg gtttccgtca actggcgcgg gaatatcccg atctggtaca
     5641 ggaagcgcgt ggtaaaggga tgttgatggc gattgagttt gttgataacg aaatcggcta
     5701 taactttgcc agcgagatgt tccgccagcg cgtactggtg gccggaacgc tcaataacgc
     5761 caaaacgatc cgcattgaac cgccactgac actgaccatt gaacagtgtg aactggtgat
     5821 caaagcggcg cgtaaggcgc tggcggccat gcgagtaagt gtcgaagaag cgtaatatca
     5881 aatcggatgg cgatacgacg tcgccatccg atctttttat acgacgcgta cgcccgcagg
     5941 catcatccgc tccggcgtta acagcacgct ttcactgccg tcatcggttt ccgcacacaa
     6001 cagcatgcat tccgacgttt caccacgcat tttcgctttt tgcagattgc ataagaccac
     6061 caccgttttt cccatcagct cctcttcgct gtagtacggc accaggctgg tcacggtttg
     6121 cagtgttttt tcccccacat caacctgtac gatgtacagc ttgtcggcgt tttcatggcg
     6181 tttcacttcc acaatctttc cgacgcgcat ttccagacgt gcaaaatcag cgtaagccac
     6241 ggtttccatt gcttcctccc ttcaagtaaa attttactaa actatagcaa aattttttct
     6301 caatcctgta ggctaaaaat ggagaatgca ggcgtgatca cattcgtaag ccgctgtgtt
     6361 accgttacag cgtcaaagaa acgcgcttta tttactgaaa acaggtgacc cgataagcac
     6421 ttcctctaca atgggggcgc acatcaggga aagtaaaaaa ggtaaacatg gcaacattaa
     6481 aagacatcgc aatcgaagct ggcgtatccc tggcgacagt atccagggtc ttaaatgacg
     6541 atccgacatt gaatgtgaaa gaagagacga aacatcgcat tctcgagatc gccgaaaagc
     6601 tggagtacaa gaccagtagc gcccgtaaac tacagacagg cgcagtcaac caacaccata
     6661 ttctggctat ctacagctac cagcaggagc tggagatcaa cgatccttac tatctggcga
     6721 tccgccacgg cattgaaact cagtgcgaaa agctggcgat cgaactgacc aactgttatg
     6781 aacacaacgg attaccagac attaaaaacg tcaccggtat tttaattgtc ggcaaaccca
     6841 cgcccgccct gcgcgccgct gccagcgcgt tgaccgacaa tatctgtttt atcgactttc
     6901 acgaacccgg cagcggttac gatgcggtgg atatcgatct ggcacgcatc agtaaagaaa
     6961 tcatcgactt ctatatcaac cagggcgtta atcgtattgg ttttattggc ggtgaagatg
     7021 agcctggcaa ggcggatatt cgtgaggtcg cctttgcgga atatggccga ctgaaacaag
     7081 tggtacgcga agaggatatc tggcgcggcg gtttttccag ttcgtcgggt tatgaactgg
     7141 caaaacaaat gctggcgcgg gaagactatc cgaaggcact gtttgttgct tccgattcca
     7201 ttgctatcgg cgtactgcgg gcaattcatg aacgtggcct gaacatccca caggatattt
     7261 cgcttttcag cgttaacgat atccccaccg cgcgatttac ctttccgccg ctctccaccg
     7321 tgcgcatcca ttccgagatg atgggaagtc agggcgttaa cctggtgtat gaaaaagccc
     7381 gcgatggtcg cgcgctgccg ctgctagtct tcgttcccag caaattaaaa ctgcgcggca
     7441 cgacccgtta aatcccctta cacactgtcc ggcaatcatt tttgccggac a
//
LOCUS       DAZPDU010000200         7424 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZPDU010000200 DAZPDU010000000
VERSION     DAZPDU010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7424
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.202"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(83..1591)
                     /gene="putP"
                     /locus_tag="ACHURS_003878"
     CDS             complement(83..1591)
                     /gene="putP"
                     /locus_tag="ACHURS_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFR1239498.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALLAGAASTILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEN"
     gene            2013..5975
                     /gene="putA"
                     /locus_tag="ACHURS_003879"
     CDS             2013..5975
                     /gene="putA"
                     /locus_tag="ACHURS_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFR1239499.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLENLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLVDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELHKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(6015..6653)
                     /gene="rutR"
                     /locus_tag="ACHURS_003880"
     CDS             complement(6015..6653)
                     /gene="rutR"
                     /locus_tag="ACHURS_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFR1239500.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFNQTVENVQRIIIEGIRPR"
     gene            6941..>7424
                     /locus_tag="ACHURS_003881"
     CDS             6941..>7424
                     /locus_tag="ACHURS_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFR1239501.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPVYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     D"
BASE COUNT         1775 a         2057 c         1942 g         1650 t
ORIGIN      
        1 ccattcaatt tatatggcga ggcacattaa gggagcgcaa cccgctgctg aagccaaaca
       61 aaaccgccgc aggctaagtc ccttagtttt cctgcaaccg tgacggcgga gccgaatgat
      121 agtgcgcatc ggcctcggca aagcgttttt gcatcgccgc tgacggcgct ttacccagca
      181 aactaaacac tacaatcccg atactgccga agatgaagcc cggaataatt tcgtacagtc
      241 ccagccagcc gaactgcttc cagacgataa ctgtcagcgc accgatgatc atccccgcca
      301 gcgcaccgtt gcgcgtcatg cgtgaccaca tcaccgagaa cagcaccacc ggaccaaacg
      361 ccgcgccaaa gcctgcccac gcgtagctca ctaagcccag cacgcggttt tccgggtttg
      421 ccgccagcgc aatcgccacc agcgccacca ccagcaccat cacacgtcct acccacacca
      481 gctctttctg gctggcctgt ttacgcagaa acgctttgta taaatcttcg gtaatcgcac
      541 tggagcacac cagcagctgg caacttaagg ttgacattac cgccgccaga atcgccgaca
      601 gcagaatccc ggcaatccac gggttaaaca gaatttgcgc cagttcgata aacacgcgtt
      661 cagcgttctg gtttaccgca cctgctaccg ccggatgctc gttaaagtaa gcgataccaa
      721 agaagccaac agccaccgcc cctgccaggc aaaggatcat ccaggtcata ctgatacgac
      781 gcgcatggac aatgctgtgg tgagaatctg ccgccataaa acgcgccagg atgtgcggct
      841 gcccgaagta acccagcccc caacccatca atgagataat ggcaacaaag ttcagccctt
      901 tgagcatatc cacgttttcg atgctctttt gtttgatcac ttccagcgag tcaccaaagc
      961 caccgacact gataatgacg ataaccggcg tcaggatcag ggcaaaaatc atcaggctgg
     1021 cctgtacagt gtcagtccag ctcaccgcga ggaaaccgcc aataaaggtg taaaggatcg
     1081 tcgacgcagc tcccgccaac agagccgttt cgtaactcat gccaaaggta ctttcaaaca
     1141 gacgcgcgcc tgccacaatg cccgaagcgc aataaatggt gaagaacagc aaaataacca
     1201 gcgcggagat aatgcgcaaa atgcggcttt tatcttcaaa acgcccggtg aaataatccg
     1261 gcaatgttaa ggcgttatta ttgtattcgg tatgcacacg caaccgcccg gccaccagct
     1321 tccagttgat ccacgcgcct aatgtcaggc caatggcgat ccagctttcg gaaatcccgg
     1381 aaagaaaaac cgcgccaggc aaccccatta acagccagcc gctcatatcc gacgcacccg
     1441 ccgataatgc cgtcacgaat ggcccaagac tacgaccgcc cagaatatag tcgtcaaagt
     1501 ttttcgttga tcgccaggcg ataaacccaa tcaatatcat gccaaagata tagacacaaa
     1561 atgtcaccaa catcggtgtg ctaatagcca tctaaagtct ccaaaaaatt attatcggca
     1621 atgtcgaaac ttgccgttat atctgccacc ggaacggggt aacagagttt atgttttacc
     1681 agggcgaccg tatcctgccg gaagcgctgg ttattcacaa tcgatttaac acaccattta
     1741 cattaaattt tagtgctcag cgacactatt tttcatcagg ttgcactctc tcacattttt
     1801 tgcggttgca cctttcaaaa aggttaactg ccgcagagaa aaagtctgag ttatttttta
     1861 atcactgtca tatcgatttc ttttattaac atttcattca tttttaagct tgctacgcaa
     1921 gtcacattta acatggttgc acaaagttgc aacatcatgg atatttcacg ataacgttaa
     1981 gttgcacctt tcagaacaac aggagtaatg gcatgggaac caccaccatg ggggttaagc
     2041 tggacgacgc gacgcgtgag cgtattaagt ctgccgcgac acgtatcgat cgcacaccac
     2101 actggttaat taagcaggcg attttttctt atctcgaaca actggaaaac agcgatactc
     2161 tgccggagct acctgcgctg ctttctggcg cggccaatga gagcgatgaa gcaccgactc
     2221 cggcagagga accacaccag ccattcctcg actttgccga gcaaatattg ccccagtcgg
     2281 tttcccgcgc cgcgatcacc gcggcctatc gccgcccgga aaccgaagcg gtttctatgc
     2341 tgctggaaca agcccgcctg ccgcagccag ttgctgaaca ggcgcacaaa ctggcgtatc
     2401 aactggccga taaactgcgt aatcaaaaaa atgccagtgg tcgtgcagga atggtccaag
     2461 ggttattgca ggagttttcg ctgtcatcgc aggaaggcgt ggcgctgatg tgtctggcag
     2521 aagcgttgtt gcgtattccc gacaaagcca cccgcgacgc gttaattcgc gacaaaatca
     2581 gcaacggtaa ctggcagtca cacattggtc gtagcccgtc actgtttgtt aatgccgcta
     2641 cctgggggct gctgtttact ggcaaactgg tttccaccca taacgaagcc agcctctccc
     2701 gctcgctgaa ccgcattatc ggtaaaagcg gtgaaccgct gatccgcaaa ggtgtggata
     2761 tggcgatgcg cctgatgggc gagcagttcg tcacgggtga aaccatcgcg gaagcgttag
     2821 ccaatgcccg caagctggaa gagaaaggtt tccgttactc ttacgatatg ctgggcgaag
     2881 ctgcactgac tgccgcagac gcacaggcgt atatggtttc ctatcagcag gcgattcacg
     2941 ccattggtaa agcgtctaac ggtcgtggca tctatgaagg tccgggcatt tcaatcaaac
     3001 tgtcggcgct gcatccgcgc tacagccgcg cccagtatga ccgggtaatg gaagagcttt
     3061 acccgcgtct gaaatcactc accctgctgg cgcgtcagta tgatattggt attaacatcg
     3121 atgccgaaga ggccgatcgt ctggagatct ccctcgatct gctggaaaat ctctgtttcg
     3181 agccggaact ggcaggctgg aacggcatcg gttttgttat tcaggcttat caaaaacgct
     3241 gcccgttggt gatcgattac ctggttgatc tcgccacccg cagccgtcgc cgtctgatga
     3301 ttcgcctggt gaaaggcgcg tactgggata gtgaaatcaa gcgtgcgcag atggacggcc
     3361 ttgaaggcta tccggtttat acccgcaagg tgtataccga cgtttcttat ctcgcctgtg
     3421 cgaaaaagct gctggcggtg ccgaatctaa tctacccgca gttcgcgacg cacaacgccc
     3481 atacgctggc agcgatttat caactggcag gtcagaacta ctatccgggt cagtacgagt
     3541 tccagtgcct gcatggtatg ggcgagccac tgtacgagca ggtcaccggg aaagttgccg
     3601 acggtaaact taaccgaccg tgtcgtattt atgccccggt tggcacacat gaaacgctgc
     3661 tggcgtatct ggtgcgtcgc ctgctggaaa acggtgctaa cacctcgttt gttaaccgta
     3721 ttgccgacac ctctttgcca ctggatgaac tggtcgccga tccggtcact gctgtagaaa
     3781 aactggcgca acaggaaggg caaactggat taccgcatcc gaaaattccc ctgccgcgcg
     3841 atctttacgg tcacgggcgc gacaactcgg cagggctgga tctcgctaac gaacaccgtc
     3901 tggcctcgct ctcctctgcc ctgctcaata gtgcactgca aaaatggcag gccttgccaa
     3961 tgctggaaca accggtagcg gcaggtgaga tgtcgcccgt tattaaccct gcggaaccga
     4021 aagatattgt gggctttgtg cgtgaagcca cgccacgtga agtagaacag gcgctggaaa
     4081 gtgcggttaa taacgcgcca atctggtttg ccacgcctcc ggctgaacgc gcggcgattt
     4141 tgcatcgcgc tgccgtgcta atggaaagcc agatgcagca actgattggt attctggtgc
     4201 gtgaagccgg aaaaaccttc agtaacgcca ttgccgaagt gcgcgaagcg gtcgattttc
     4261 tccactacta cgccggacag gtgcgggatg atttcgctaa cgaaacccac cgtccattag
     4321 ggcctgtggt gtgtatcagt ccgtggaact tcccgctggc tattttcacc gggcagatcg
     4381 ctgccgcact ggcggcaggt aacagcgtgc tggcaaaacc ggcagaacaa acgccgctga
     4441 ttgccgcgca agggatcgcc attttgctgg aagcgggtgt accccctggc gtggtgcaat
     4501 tgctgccagg tcagggggaa accgtgggcg cgcaactgac gggtgatgat cgcgtgcgcg
     4561 gggtgatgtt taccggttca accgaagtcg ctacgttact gcagcgcaat atcgccagcc
     4621 gcctggacgc tcagggtcgc cctattccgc tcatcgctga aaccggcggc atgaacgcga
     4681 tgattgtcga ttcttcagca ctgaccgaac aagtcgtcgt ggatgtgctg gcctcggcgt
     4741 tcgacagtgc gggtcagcgt tgttcggcac tgcgcgtgct gtgcctgcaa gatgagattg
     4801 ccgaccacac gttgaaaatg ctgcgcggtg caatggccga atgccggatg ggtaatccgg
     4861 gtcgcctgac caccgatatc ggtccggtga ttgatagcga agcgaaagcc aatattgagc
     4921 gccatattca gaccatgcgt agcaaaggcc gtccggtgtt ccaggcggtg cgggaaaaca
     4981 gcgaagatgc ccgtgaatgg caaagcggca cctttgtcgc cccgacgctg atcgaactgg
     5041 atgactttgc cgaattgcac aaagaggtct ttggtccggt gctgcatgtg gtgcgttaca
     5101 accgtaacca gctaccagag ctgatcgagc agattaacgc ttccggctat ggtctgacgc
     5161 ttggtgtcca tacgcgtatt gatgaaacca tcgcccaggt cactggctcg gcccatgttg
     5221 gtaacctgta tgttaaccgt aatatggtgg gcgcagtggt tggtgtgcag ccgttcggcg
     5281 gcgaagggtt gtccggtacc gggccgaaag caggcggtcc gctctatctc taccgtctgc
     5341 tggcgaatcg cccggaaagt gcgctggcag tgacgctcgc gcgtcaggat gcagagtatc
     5401 cggtcgatgc acagttgaaa gccgcattga ctcagccgct aaatgcactg cgggaatggg
     5461 cagcaaatcg tccagaattg caggcgttat gtacgcaata tggcgagctg gcgcaggcag
     5521 gaacacaacg attgctgccg gggccgacgg gtgaacgcaa cacctggacg ctgctgccgc
     5581 gtgagcgcgt gttgtgtatt gccgatgatg agcaggatgc gctgactcag ctcgccgccg
     5641 tgctggcggt gggcagccag gtactgtggc cggatgacgc gctgcatcgt cagctagtaa
     5701 aggcattgcc atcggcagtc agcgaacgta ttcaactggc gaaagcggaa aatataaccg
     5761 ctcaaccctt tgatgcggtg atcttccacg gtgattcgga tcagcttcgc gcattgtgtg
     5821 aagccgttgc tgcgcgggat ggcgcaattg tttcggtgca gggttttgcc cgtggcgaaa
     5881 gcaatatcct tctggaacgg ctgtatatcg aacgttcgct gagtgtgaat accgctgccg
     5941 ctggcggtaa cgccagttta atgacgatcg gttaaacagg tagccggagg atgttacaac
     6001 ctcctccggc atctttaacg tggtcgaatc ccctcaataa taatccgctg cacgttttca
     6061 accgtttgat tgaaaaatat ctcatcgcgc aacgtcgcgc ctgtcaccgc ctccacctga
     6121 ggggcgaaat cggcgtaatg ttgagtggaa gcccaaatca tgaaaatcaa atgctgcgga
     6181 tcaatcggcg cgagtttgcc gcttttgacc caaccggcaa tcagcgcaga tttctcatca
     6241 attaatgcct tcaaatcgcc cgtcagttca tccattaaca gcggcgcgcc tgccagcatc
     6301 tccatacaga acaggcgcga agcctgcgga taatcgcggg agacttccag cttcagacgg
     6361 atgtactctt ttatcgccgc cagcggagcg aaatcttcac gaaacgcttt taacggtgcc
     6421 agccagatat cgagaatctg ccgcagcacg gcaatataca gcgcctcttt tgacggaaag
     6481 taatacagca gattggtttt tgaaacaccc gccaactctg cgatctgctc cagccttgtg
     6541 ccgtgaaaac cgaattgtga aaaagtgtcc agtgctgcgc taagaatcgc ttttttcttc
     6601 gcgcttactg cgcgcgaacg tttacccgtt gttttcactg cgccttgcgt catgcgctct
     6661 cccctcttgt agattggcaa caggatagca aaacccccgc ccggcctcga ccttctgcac
     6721 tctcatcgcg cacagatgca tgttttatgt gcaactgttt tgaccgttta gtccactttt
     6781 tatcagatat tttagataac gtaatattaa catattaata acattaactt tttaaaaact
     6841 ggcatccgct ttgcaaacaa gccaatacgc ggctgaaaag gatatgcagg atgcagcgcc
     6901 ccgtctgact ttcactttac gagacgaaga gaggttgatg atgaaaattg gcgtattcgt
     6961 acctattggc aacaacggct ggctcatttc gacccacgcg ccgcagtaca tgccgacctt
     7021 tgaactgaat aaagccattg tgcagaaagc ggagcattac catttcgatt tcgccctgtc
     7081 gatgatcaaa ctgcgtggct ttggcggcaa aactgagttc tgggatcaca accttgagtc
     7141 gttcaccttg atggcgggtc tggcagccgt gacctcgcgc attcagattt acgccactgc
     7201 tgccacctta acgttacctc cggcaatcgt cgcccgtatg gccgcaacca tcgactccat
     7261 ctctggcggg cgttttggcg tcaacctcgt gactggctgg caaaagcccg tgtatgagca
     7321 gatgggtatc tggcctggcg atgactattt ctcccgtcgt tacgactatc tcaccgaata
     7381 tgttcaggtg ctgcgcgacc tgtggggcac ggggaaaagc gatt
//
LOCUS       DAZPDU010000201         7388 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZPDU010000201 DAZPDU010000000
VERSION     DAZPDU010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7388
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.203"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(1..4520)
                     /locus_tag="ACHURS_003882"
                     /pseudo
     CDS             complement(1..4520)
                     /locus_tag="ACHURS_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616597.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            complement(4540..5001)
                     /locus_tag="ACHURS_003883"
     CDS             complement(4540..5001)
                     /locus_tag="ACHURS_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFR1239502.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(5029..6931)
                     /locus_tag="ACHURS_003884"
                     /pseudo
     CDS             complement(5029..6931)
                     /locus_tag="ACHURS_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243511.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
BASE COUNT         1419 a         2231 c         1978 g         1760 t
ORIGIN      
        1 gtttatttga ctacgtgata aacgaacccc atccaggaaa cccgtatgcc cactaacacg
       61 acgaccatcg ccaatatagt ccatcaaact gctgatttta gaacttgcag ccaatcccag
      121 cggatcaacc cacccacaag gattcggcac ataccggtaa gtctgctccc cgccctgcaa
      181 ccccagcgga tcactcacca ggtacattcc ggtttccggc tcgtaatacc ggaaccgatt
      241 atagcacagc ccggattccg catcctgcca ctgtcctgca tacagcaggc cggggtcggc
      301 ttccgggtcc agttccccgc gcgggtccgg gtagccggtg tctgcgggca ggctgagtgc
      361 cagcccccac aggctggtct gccccggtcg ccagaccgtc tttccttcac tgttaaagag
      421 catcagcggg cggcccgtca ggtcgctcac cgcatgattc gtccgcgtca cccactgcgg
      481 ggccaccgac ggatgcgcct gccgtacccg gctgaactgc tggcttatca gctcaaagcc
      541 gttaaacacc aggtgcctta cgctgtacag tttatcatcg cggtattccc ggatttccgc
      601 aatactgtcg ccgtcccaca gatacgtgaa gcggattttc ttccggtcgc agcgtttctc
      661 tgttcgtctg ccgctggcgt cgtggcggta ttcccacagc tcaccctgtg cgctgcaata
      721 cccggtcagc tggtcccggc tgtcccaccg gaaccgctct gtttccggtc ggtagccgtc
      781 acggtgtctg gtgcggctga ccatccggcc tgcggcgtca tagtcatact gcgtgtttcc
      841 ggcctgtttc agccggtggc ctccggcata ctggtcagtc tcttcactta tccggtgcct
      901 gcccgcagac tgcgccttca ggtagccgca gctgtcatag ccataggact catcgccttc
      961 gccatagccc agcccggtgc ctgaggctgt caccgccgtg acctggccgt tccggtccag
     1021 ccgatactcc cgccgtgttt cccgtccgta atcttcccgg ctggtgacgc ctgacacgtt
     1081 acccgcgctg tcgtaacggt actcccgtac cagtgaggga accagcccgt cccatgtggc
     1141 ttctgccgga aaaaatccgg cgcgctgact gacaggctga ccggctgcat tccagccctg
     1201 ttcacaggca aaaccccggt tactgtgccg gagcacttca cgtcccgcct catcacggct
     1261 gaacgtcagc gtctcatccc ccggcagaat gacctctgtc agttctccgg cggcgttgta
     1321 gcggtacgtg gttttcagca gggattcacc ggggtgtctg tcgcgttcat cttccggtgg
     1381 caggatacag cggatgacgg tgtgttcatc ggggtagttg tattcaatgg cccgtcggcc
     1441 ctgagtggtg acattcaccc gcccgcgttc gtcatagccg gtgcgctgtg atgtgccgtc
     1501 cgggctgaag atacccgtca gcctgccgga tttgtcatag gcatactgcg ttttgccttc
     1561 cggcgagatg atttctgcca ggcggcctgc ggcgtcccgg ctgtagagga tggtttcctc
     1621 caggccgtta accctggcgg tacagaggcc atcggcgtca tagcggtaac gccagaccac
     1681 gccgccccag tcacgctctt ccgtcacccg gccgcagtta tccagaacat aaagatatga
     1741 ctcaccctgc gggttgatga cttctgtcag ttgcagcgtg tctttatcgt agcggtactg
     1801 ccagcggtgg ccttcaccgt ccgttcgcgc caccggaaaa tcgaacggac cgtattccat
     1861 cgtccagaca acaccgttgc cgtccgtcca ggccagcagg ttgccgtggc ggtcatagcg
     1921 aaatccttcc gcgccggttc cagcacgttc aatgccgctc agtttatggt gctgctgcca
     1981 ctgtaaccgc actgactgac cgccggggcc gcgcatcttc actggcaggt cgtgaccgct
     2041 gtattcggtg tggtgttcac ggcccatcac gtcggtgagt gtttccggcc ggttgtggtg
     2101 gtcgtactct atgcccagac gccgttcatc ctggcggaat attcccgaca gcagcccctg
     2161 agcggtgtat gccagccggg tctcttcgcc ctgtggcgac accagactta ccggcaggcc
     2221 gcgttcatca cactgctgtg tccacgtctg cccggcaggg tcggtgatgt tgctgagcag
     2281 gccgcgctca tcatagcggt actgccactc cgcgccgtct gccgccgtgt aacggcttaa
     2341 ctgaccgtgt gcggcgttgt attcaaattt ctccacccgc ccggcagggg agtggcgggc
     2401 cagcaggtga tggaattcat cccattcata catggtggtg ctgccgtccg gcgcttcttc
     2461 ccgcagaata ttgccgtcag gatcatagta ataacggtga atgcccttgc cgtccgtcac
     2521 ggtggtgcag ccgtcaccat agtccagcgt ggcgttgtaa tagccctccg ccccggtgac
     2581 attcacgcac cggccctgtt catcgtaggt gaaacggctc cacgtctggt cgttatcgga
     2641 ccagcggatg atccggtccg cagcgtcgta ctcataaaac aggtggtagt ccagccgcgc
     2701 atccgcttct gtcagccgcc cccgcgcatc ctgttcgtag gtcgccagcg tctgtatgtc
     2761 gttatccgtg cggtgaatgg ctttcaggta gccgctggcg tggtacgtca gtttaagccg
     2821 gatcccgtcg gtgtgactga cacccgtgat ccggtactgg ctgtcgcgct caaacgtgat
     2881 gcggttgctg gcggtgtcgc tcatcccgcc cagcatgtac atccggtcat tggggctgtg
     2941 tgccaggcgg tcatcgctgg tatcgtcagt caccgggtgg cgcagacgca ttcccggaaa
     3001 ggcgtcataa aagaagcggc tgctcagtgc atccctgtcg aacagctcca gataatcatc
     3061 gcggcggcgc agaatgtact gggcgtgata cgggttaacc gccgtatcac tgtcaccgtc
     3121 gaaggcaaat ctcagcgtgg ctccctggct gatgatatcc acccggtttc cctgttcacg
     3181 cacccaggcg tattcgctcc aggagtcgct ccagcccacg ccggtcaggc catgcagcgg
     3241 cagtacagaa ctgtggaaac gggtgaagga gagggggagg gtctggccca gggtgaagtc
     3301 ggtacgttcg tcgcagtatg cgccggttac cggatcgaca ggatctgtta cccatctgat
     3361 gactttattg gcaatacttt ttaacgactt acctgttttc cccagcaacg cgccgagctt
     3421 tgcgcccagc agcttcccgc ttttccccag cagtttccca gtttgccgag tctcgccaga
     3481 ttcttcagtt ttcccagtag tcccgctggc ggcagaagat agctggctat cgttattccc
     3541 cccaggatca ttctctgcca cggcgggaac tccggctgga tatccagata tgtgacctgc
     3601 tcaccgccaa taaagacgtc cggcgaacct cccacgatcg ccgcactgca ctccagtttg
     3661 tcgcccttgc gcgcggcagg ctgattgttg ataaagacgt tgctgctgcc ctgggctatc
     3721 ctcggtggtt tgttctcctt gtcgcagagc gccgtgtcct caccggcttc cgggaaatcc
     3781 acatcttttt tgatttcgcc acgcccgccc accatggtgg caaactccat taataccgga
     3841 ccggcgggaa acagaagcga tgcgccggcg ctgacgcgat ccataaaacc ggcattgctg
     3901 tccgtcaggg tgataacagc gttcccccag gccttcgcca gtggtactgc tttttccgct
     3961 gccgattcaa gcaggtcagt ggcaatatca ccccatgagg gttccccctg tgggacttca
     4021 ggtattgctc caggtggggg aggaggggga agggtgaccg ctgcacgtgc ggccagctcg
     4081 ccgttaattt ttacgttttc agaaccggta ttgatggcgc cgtccgtgtc acaaaagctg
     4141 tttatccatg attcaagctg gttctgtttc tcctccacat aatcactcgc cttatagctg
     4201 gcatacatca gcgcaggtac cagaaccagc gcataagggc cggtggcaca ggcggccata
     4261 atcaggcaac cttctgcgat ggtcactatc gcccccagta cggcccctgc cagcgccccc
     4321 aaaaaactct tgtgctgaat ttcatcgccc agcctggcgg cgggggggcc agactttttt
     4381 gatgccaaca tctgtgcaat ggctttgtcg ttggctgccc gtttcatctg gctggcgacc
     4441 atcgcctgct gtgacagcat tcgcattgcc agcgtaccgc ctgcggtcgc tccctgtggc
     4501 ccgcctggtc cttcactcat gtgttatccc tcgtttatgt cagcgcggct ggtccttttg
     4561 gatgtcattc tccggtggca acggggtgaa gctctttatc acctccagca gcatcgtctt
     4621 ttgactgttc ttcagggata aggaggtgct gaaagtgaag gtcagcagcg tatctccacg
     4681 ccgctggaat acggctatct ggctaatccg cccctgattc ccctcatctt ccggaccgtt
     4741 ccaggaataa tccagcagcc aggccggggc ctctgccaga gtgatttccc ggcaggcatg
     4801 ttcggtgtac ccatcaagtt tgccctggta cagcgccagc gtttcctggt acagctctgg
     4861 tgtcagggtt ccctgtggaa cggcttcgcg ggtaatgacc aggttgatgc cactgttatc
     4921 tgtggacacc aggacctgca ttgactgatc ctgccattga gacggcagtt gaatgtggcc
     4981 ttcattaaaa gtgaactgca taaaatactc cctgataatc tgagtgaatc agttgatgaa
     5041 aacagaaccg cccacgatgg tgatgccatc cttcgacagc ctgattaccg tcgattcccc
     5101 gacctgtagc tgaatgctat ccggcgtcag cgcaattttg ctttcctcca cggccagcaa
     5161 aatttcctta atcgccgtat aagcctgtat accgtcgcct tcgccaccat tgtttacagc
     5221 gtcaacatgc accgaaccgg caatcgtgcc tctgtcctga aagacatcct cagataaaga
     5281 ccctttcagt actccggtga tcttgtggcc tttttccacc gtaagaaggc ggttaccttc
     5341 ctggatcgtg accgtctggt cattttttac ggtttcgcgg tgatcgtgtt ttacggtggt
     5401 ggtacggtcg ttgagcactt cggtatccat gttcttctgc gcgtggatgt agacctgttc
     5461 tctccctgtc gcatcatcaa acttcagctc attaaatccg ctgcccatgt acgttttgga
     5521 gcggatggtc atctgcgtct ttgttcccgg caggctgccg gggggggcgg ttttcctggt
     5581 ggtaggtgcg ccccataatg atcggctggt ccggatcgcc gttgaggaag tctacaatca
     5641 cttcctgacc cacacgcggt atcgccaggt ggccaaaacc ggtgcctgcc cacgcctgtg
     5701 ccacacggat ccagcacgaa ctgtcttggt ctgccgggtt ataacggtcc cagttgaact
     5761 tcacccgcac gcgaccatgt tcgtcgcaga agatttcctc gcctgccggt cctgtcacga
     5821 cagcgctctg cgggccgtcg accagcggtt tcagcaacgg ctgtggtcgc catgttctgt
     5881 ctgccgggat caccgcaaaa tggttctcca gcgccgtccc cgctccctgc cgtcctggca
     5941 ccgcctgtgg ctgttcgccg tgcagttcac ttgccaccac ctgccattcc cggttcaggt
     6001 tcgcctgcgg atgccccgtc agcacaattc gtcgtcccgg ccatatctcc ggcgagcgac
     6061 tcattccccg cgcggtttct gcattgtttc gccagccgtc catctgccag cgggcaaagt
     6121 tctgcccgtg agcacccttg aaacgtccgg ggtagtcata cacttcatac tgcgtgcgct
     6181 ggtagtcctg gtgctggcct tcctgttcaa aacgtccggc ccagccgggg cgtttaaagg
     6241 tgtagtcttt ggtcaccacg gaagaagggc ggatttgtgc gctgtagcgg aactggctga
     6301 tgcagagggt gctcacctcg gtacgggtgt tcgggttcca tgggatttca aaagattcgg
     6361 gcagatggcg gactgtgtcg cacagcacca ggctctggtc ggtacttttg taagcatgct
     6421 cctcataaaa gaagatgcct tcctccgccg ccatccggca caggaaatcg taatcagtct
     6481 caccgtactg gacacaaaac tcacgggaag gatgcggctc gctgaacagc ggactccatt
     6541 cggtcacccc gttttcctgc aacatcgtgc cgaggatgct tttgatgtcc tcgttctgga
     6601 agatacggaa gttctggcgc agaccggcac gccacagcgg cgggtgcacc ttcatgctgt
     6661 acagcatctg gtttttgtcg ttctccccga gttcaaacca ggtcaccacg cctttcaccc
     6721 ggcgctgtac ttcatcgccc tgccatatcg tcaggtaggc cattttgtcc agcacctgcg
     6781 caaattcaag ggagagaaac tgctggctga ccagggagag atcgagggaa aagagcgaag
     6841 agagtgactg gttcagatga aaggaaacca ccgcaaaagc gtccggcggc aggccgtcca
     6901 cttccagtgt gaaacgtaat ccggttgaca tagcaacctc cagtaaaatg aataatgatc
     6961 cgtaccgtac aactgcggat aatgtgataa gcgggacgac ttcagacgtt attagggaag
     7021 gaacagaaaa tgctatggtt atgtgagaat tttgtttaaa tattacttta cagaaacggg
     7081 tgtctgttca gtcaaaaaaa taatcacaag ctcaacctct tgtttcttgg cgttattcag
     7141 gacaggtacg catatcacaa cgagtaacaa aaacgcaaag ttaaaccaat attcataata
     7201 tatttttatt cacagcaata aataatccaa ttaacattat taaaatgaat gattgcataa
     7261 gaaatatatg ctaccgctcc acccatgata acatgatctg cacattgcac atcttaacgt
     7321 tgttaaatac tggctcaata gtggatattt taaattagga atttatcaaa ttcacttgac
     7381 aaatagat
//
LOCUS       DAZPDU010000202         7354 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZPDU010000202 DAZPDU010000000
VERSION     DAZPDU010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7354
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.204"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            86..421
                     /locus_tag="ACHURS_003885"
                     /pseudo
     CDS             86..421
                     /locus_tag="ACHURS_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            418..873
                     /locus_tag="ACHURS_003886"
     CDS             418..873
                     /locus_tag="ACHURS_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239503.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYLMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(951..1862)
                     /gene="rdgC"
                     /locus_tag="ACHURS_003887"
     CDS             complement(951..1862)
                     /gene="rdgC"
                     /locus_tag="ACHURS_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFR1239504.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFALRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            1987..2895
                     /gene="mak"
                     /locus_tag="ACHURS_003888"
     CDS             1987..2895
                     /gene="mak"
                     /locus_tag="ACHURS_003888"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFR1239505.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVALNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            complement(3038..4222)
                     /gene="araJ"
                     /locus_tag="ACHURS_003889"
     CDS             complement(3038..4222)
                     /gene="araJ"
                     /locus_tag="ACHURS_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFR1239506.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISVFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(4348..>7354)
                     /gene="sbcC"
                     /locus_tag="ACHURS_003890"
     CDS             complement(4348..>7354)
                     /gene="sbcC"
                     /locus_tag="ACHURS_003890"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFR1239507.1"
                     /translation="LDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGE
                     AYRAFWSQNRARNQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFT
                     RSMLLSQGQFAAFLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQ
                     ASGVTLLTPEQVQSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQ
                     ALQQALAEEEKAQPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQS
                     TMALRASIRHHAAKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDRE
                     HLRQWQQQLTHAEQKLNALAAITLTLTADEVASALAQHSEQRPLRQRLVALHGQIVPQ
                     QKRLAQLMVTIQNVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQ
                     RAQLQAGQPCPLCGSTSHPAVEAYQALEPGVNQSRLLTLENEVKKLGEEGAALRGQLD
                     ALTKQLQRDENEAQSLRQDEQALTQQWQAVTTSLNITLQPQDDIQPWLDAQDEHERQL
                     RLLSQRHELQGQIAAHNQQIIQYQQQIEQRQQQLLTALTGYALTLPQEDEEESWLATR
                     QQEAQSWQQRQNELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLA
                     LHSQQQTLQQQDVLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLK
                     QNLENQRRQAQTLVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTS
                     QGEIRQQLKQDADNRQQQQTLLQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLT
                     LDNLVHLANQQLTRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSL
                     ALALALSDLVSHKTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEA
                     MKERIPVQIKVKKINGLGYSKLESTFAVK"
BASE COUNT         1698 a         1907 c         1906 g         1843 t
ORIGIN      
        1 tcctttgctg cgattttttc aatttccgat atattcataa ttcatcaagg ttgatataaa
       61 tatcagtgaa gatctccagg tattttttgc ggaactggct acgataaaag ataaatcaga
      121 tgatgaatgg tggcgtgcat tgctgcaaag tcgcaatgat catttgcgcc aaacggcatt
      181 acgcaacgca cataccccgg cgtcgttgtt aacgacattg actgagtctc aggatcggtc
      241 gctggctatt aataatccac agctggctgc cgatgtgaaa acggcgtggt taaaagagga
      301 tctatcatta ctcttatttg tcgaacaacc cgatctttcg cagttacgtg atttagtgaa
      361 aaccggggca acgcggaaaa ttcgcagtga agcgcgtcac cggcttgagg aaaaacaatg
      421 acacaacgtc catggagcaa gttacagcgg gagatctatg acttactgac gccaacgatc
      481 aacctgcaaa ttcactgtac ccgttatctc atgcgcagcc aaaatggtgg aagtaccgat
      541 ttaccgcgtt attggatcac gttggataaa aacgtgatct gggattaccc gaaagacttt
      601 attgcaggta acgctggagt gcgtaatttt cacggtgaaa cttgctggta tccttacctg
      661 acggatattt gttcaatttc tgatttattg cgtgaatata ttgatacccc aaaagcggag
      721 ctgttaacaa aacaatttac cagcgataaa tgggggttag tgaatattct gcgtgctgct
      781 gacaggcgaa tagggatgag gcgattagat cagttacggc gtaaaacgca taatattgct
      841 gcgctaaaaa ttattgctcg ccgtagcgaa taattatatg cctggtgtgg cttcgtacgc
      901 cggataagac gcggcaggcg tcgcatccgg cattaaagga aaatcagcaa ttaacgttgt
      961 gcttcgccac ctaatccttc aatcaggttt tgaattaacg ctgccagttc accagtcatc
     1021 aggatgaaat cggcatcaaa acgcagggcg aaatcttcac ggtcgatatc ttcgttttga
     1081 tcgcgcagct cgtcgcagaa cttaagacgc ttgagcgaac catcgtcgca catcacaaac
     1141 tgaatgcgct gctgccagtc gagcgccagt ttagtcacca cttttccggc ttcaatgtga
     1201 ttggtgatct cttcgctggt cagatcttgt ttcttcgcgc ggatcacgcc gccatcttcc
     1261 agcaacgatt tcagctcggc ttcatcaagc agctggaagc cctgtgccgc actaccggag
     1321 cgaacccatt cggtcagcgt cagttcaatc gggttttcca tgctcaatgg tacaaccggt
     1381 aacgacccca ggcttttacg cagtaatgcc agcgtatctt ccgctttttt ggcactggcg
     1441 cagtccacca taatcaaacc gttaaccgtg tcgatccaca tcattgtctg gctaaaacgg
     1501 ctgaaagcac gcggcagcag agagtgcagc acttcgtctt tcagcgaatc tttttcggtt
     1561 ttcttcagct tacgcgcctg ttccgcttcc agtttggcga ttttcgcttc cagcgcctgt
     1621 ttaatcaccg gagacgggag gattttttct tctttgcgcg cgcagataac aatttgacca
     1681 ttggcaacgt gcgttaacgc atcgctgtgc gatcccatcg gaggaaccca gcccatcttc
     1741 gccatgtcct ggctgccgca tggggtaaat gccatcgagg ctagctgttt ttccatctct
     1801 tctgcacgca gcgaaatctc gcggctaaga cggtaaacca ttaaattttt gaaccacagc
     1861 atgataattt ccacggcctt gtcgttaaat ttagcgggca tgataacgaa ttgtcggcgg
     1921 ccttgcattg ccaatccggt tgtccgtctc tacgctattg atattgaaaa aaataaggag
     1981 agtacagtgc gtataggtat cgatttaggc ggcaccaaaa ctgaagtgat tgcactgggc
     2041 gatgcagggg agcagttgta ccgccatcgt ctgcccacgc cgcgtgatga ttaccggcag
     2101 actattgaaa cgatcgccac gttggttgat atggcggagc aggcgacagg gcagcgcgga
     2161 acggtaggta tgggcattcc aggttcgatt tcgccctaca ccggtgtggt gaagaatgcc
     2221 aattcaacct ggctcaacgg tcagccattc gataaagact taagcgcgag gttgcagcgg
     2281 gaagtgcggc tggcaaatga tgccaactgt ctggcggttt cagaagcagt agatggcgcg
     2341 gcagcgggag cgcagacggt atttgccgtg attatcggca cgggatgcgg cgcgggcgtg
     2401 gcattaaatg ggcgggcgca tatcggcggc aatggcacgg caggcgagtg gggacacaat
     2461 ccgctaccgt ggatggatga agacgagctg cgttatcgcg aggaagtccc ttgttattgc
     2521 ggtaaacaag gttgtattga aacctttatt tcgggcacgg gattcgcgac ggattatcgt
     2581 cgtttgagcg gacatgcgct gaaaggcagt gaaattatcc gcctggttga agaaagcgat
     2641 ccggtagcgg aactggcatt gcgtcgctac gagctgcggc tggcaaaatc gctggcacat
     2701 gtcgtgaata ttctcgatcc ggatgtgatt gtcctggggg gcgggatgag caatgtagac
     2761 cgtttatatc aaacggttgg gcagttgatt aaacaatttg tcttcggcgg cgaatgtgaa
     2821 acgccggtgc gtaaggcgaa gcacggtgat tccagcggcg tacgcggcgc tgcgtggtta
     2881 tggccacaag agtaaaaaac gtaggcaatt ggcgcatcat gcctgatgcg acgcttgccg
     2941 cgtcttatca ggcctacaaa atgtgccaga accgcgtagg gcggataagg cgttcacgcc
     3001 gcatccggca ataagcaacg cgcaccgtga cagtaatcta ccccagtggt ttcgccagca
     3061 ccggactatc cgccgcttgc tggcgcttat agcgaccata cagcagcaac gatgacatcg
     3121 cagcaaacga aagcagggcg gcaggcagcg ccacgtaatt atatgccagc cccagcgtca
     3181 gcatcatacc tccgcaatat gcgccgacgg cgctaccgag gttaaacgct atttgcccac
     3241 ctgcggcacc taataactct ccgcctttgg cgttttgcag taacaatatt tgcagaggtg
     3301 ctgaaagggc aaataatccc gcgcaacaaa taaaagcaaa aataagcgac gttgttttca
     3361 tgccgccgca gaaaaagagc atcagcagtg ccagtacaat gataaagtca gtcactgctg
     3421 caatgcgcag tggtgaataa cgtcctgaaa tcctgccact tagcatattt cccagcacca
     3481 tccctagccc aactaacatc ataataaagg tcatcgccgt ttccgaaaaa acggaaataa
     3541 acatcatgta tggctttacg tagctgaacc aggcaaagac acctgcgttg ccaaacatcg
     3601 tggcggcgaa aattaaccac ggggccgggc tgcgcaaaaa gtgaaattgt tcgcgcagat
     3661 ttcctttcgc ctcgtcgcga atatctggca cccaaaaata gaccgatgcc atcaccgcaa
     3721 tattaaaaac agcgatcaat aaaaaggtgt aacgccagct aaattcctga cttaaatacg
     3781 ttcccagcgg aatgcccagc aaattggcga ctgtcatccc ggaaaccatc cccgccacgg
     3841 cggcggtgac ttttccgggt ttgataattt ttgataacac gatcgcgccg acgccaaaaa
     3901 atgcgccatg cggaaagccg gaaaccaggc gaccaatggc aagcatcagg taagacgaag
     3961 agagcgtgaa catggcgttg ccaatgacgc acaacgccac cagaaacaac aagatatgtt
     4021 tgagtgagta gcggctggaa aagagtgcga tgattggcgc accgaccacc acccccagtg
     4081 cataatacga gatcatatgc ccggcagcag gaatcgaaat tcctacgtta tgcgccagct
     4141 ccgtgagcac gcccataatg ccaaattcgg ccatccccaa accaaacgtg cccagagcca
     4201 aagataaaat gacttttttc ataccaccat cacaaccacc agaacggcta ccaatcaccg
     4261 tagccaataa agtgatagct tgcaggataa cgaccagcaa ggccaagaga taattcgccc
     4321 ctctgtattc attatcctgc tgaatagtta tttcactgca aacgtacttt ccagtttgct
     4381 gtagcccagg ccgttgatct tcttcacttt gatctgcaca ggaatacgct ctttcatcgc
     4441 ttcaacgtgg ctaatcacac cgatggtttt gccactggcg ttcagggcat ccagcgcatc
     4501 aagggcggta tccagcgttt cgctatccag cgtgccaaaa ccttcatcaa ggaacagcga
     4561 gtcaatacgt gttttatggc tgaccagatc cgaaagcgcc agcgccagcg ccagactaac
     4621 gaggaaactt tcgccgccgg aaagggtacg ggtatcgcgt actgcatctg cctgccaggt
     4681 atcaacaacc tcgacttcca gcgcctcgct ggctttgcgc tgtaacagat agcgcccgtg
     4741 cagccgggta agttgctgat tagcgagatg gactaaatta tccagcgtca gcccctgggc
     4801 aaacttgcgg aatttatcgc cctctttgga acctattagc gaattcagat atccccagtc
     4861 ctcaacctgc tgcgtcattt gagcaatttg ctgcagtaag gtttgttgtt gctgacggtt
     4921 atctgcatcc tgcttcagct gctggcgaat ctcgccttga ctcgtggtgt tttcacgcaa
     4981 cttttggtga gtttgcgcta actcttgctg aatctgctcc accgtcacag tgagagccaa
     5041 cccgtcaggt cggtgttgtt gatgctgtgc cagcgtttct gctgtctgtg tgaccagcgt
     5101 ttgcgcctga cggcgctggt tttccagatt ctgcttgagc tgttccagct gcgttagtgt
     5161 ttgttcatcc attagcgccg caaggaacgc ctgctgatcg tcaaagacgc tggcctgtag
     5221 cgcggtgtca aactgcgcct gggctttttg cagactttgc gccgccagaa catcctgttg
     5281 ctgtaacgtc tgctgctggc tgtgtaatgc gagacattgt tcatgaacct gccgccagtt
     5341 atccagcgcc acagtttctt cgctgtgcgg gagatcatca ctttgcggca acgtttccag
     5401 aatcggcgtc agctgctgaa tacggttttg cagcgcggtt aattcgttct ggcgttgctg
     5461 ccagctctgc gcttcttgct gacgtgtcgc caaccagctc tcttcttcat cttcctgtgg
     5521 caatgtcagt gcataacccg tcaatgccgt taagagctgc tgctggcgtt gttcaatttg
     5581 ctgttgatac tggataattt gctgattatg cgcggcaatc tgcccttgta attcatgtcg
     5641 ttggctgagt aaccgcagct ggcgttcgtg ctcatcttgt gcatccagcc acggttgaat
     5701 atcgtcctgt ggctgcaagg tgatattgag gctggtcgtg acggcttgcc attgttgagt
     5761 aagtgcttgc tcatcttgtc ggaggctttg cgcttcgttt tcatcgcgct gaagctgctt
     5821 cgttaatgca tccagttgcc cgcgcagcgc cgcaccttct tcgccgagct ttttaacttc
     5881 gttttccagc gtcagtaatc gagactgatt aacaccaggc tccagcgcct gatacgcctc
     5941 gaccgccggg tggctggtgg aaccacaaag tggacaaggt tgacccgcct gtaactgtgc
     6001 acgttgagct tccagcgttt tgatgcgcgc ttcctgctcg caaatggttt tcacatcggc
     6061 aagttgctgc gtcttttctt tataacgctg gcgcatttcg ttaagtgcga cattacgttg
     6121 cgtctgttcc agagtgacat tctggatagt gaccattaac tgcgccagac gtttttgttg
     6181 gggaacaatc tgcccatgca gcgcgaccag gcgctgacgc agtgggcgtt gctcggaatg
     6241 ttgcgccagg gcgctagcaa cttcatcggc ggttaacgtc aacgtgatcg ccgcaagcgc
     6301 attaagtttt tgctcagcat gggttaactg ttgctgccat tgccgcagat gctcgcgatc
     6361 gctggtttgt tgggagaact gcgcacgcca acccgccagt tcgttgttcc actgacggaa
     6421 gcggtcgtgt tcctgtaacc aggtattcag gctttgttgc tgctgctgta attctgctga
     6481 ctgcttcgcc gcgtggtggc gaatgctcgc gcgaagcgcc attgtgctct gtaagcgagt
     6541 atttacttct tcaatctgct ggcgaatatg cgccagcgcc gcgctgtgtt ctgcgatgcg
     6601 ttcccagtgt ggacgaagat ttcgtgccgg ttgtgccaga ctaagcgccg ccagttgagg
     6661 ttgcgctttt tcttcttcgg ctaacgcctg ttgcaaggcc tgctgacggc ggctggcttc
     6721 ttgctgcaat tcgtcctgac gcgttaacca gtttagcgat tgttgttctt gctgctgcgc
     6781 ggtaattaac tgtttttctt cgtcagtaag tacctgcaaa ctcgctgtca gcgattgcac
     6841 ttgttccggc gtgagcaacg tgacgccgct ggcctgcgct tgcagcttct ccagctctgt
     6901 gcgggccgat ttgtgctgct caaaaaccat cgccgagatt tgcccgtaga tttcagtgcc
     6961 ggttaactcc tcgagcaatt ccgcgcgttc tttgggtttg gcattcagga aggcagcaaa
     7021 ttgcccctgc gaaagcagca tcgaacgagt gaagcgcccg taatccagcc cggttaacgt
     7081 cgctgtcagt tccagcttat ctttcacttt gtcggcgaga attttgccgt cggcgcagcg
     7141 cgccagctct acgcgtggca cctgcaaatt accgtcgggt tggttacgcg cccgattctg
     7201 gctccagaat gcacggtacg cttcaccttt cacttcaaac tccacctccg ccagacattc
     7261 ggcggtatcg cgggtcatga gatcattttg cgattgtgaa acgttagaga gacgcggagt
     7321 ttcgtgatac agcgccagac aaatggcgtc cagc
//
LOCUS       DAZPDU010000203         7142 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZPDU010000203 DAZPDU010000000
VERSION     DAZPDU010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7142
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.205"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..268
                     /gene="rutC"
                     /locus_tag="ACHURS_003891"
     CDS             <1..268
                     /gene="rutC"
                     /locus_tag="ACHURS_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFR1239508.1"
                     /translation="NVLFADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWK
                     NYAAINEIYAEFFPGDKPARFCIQCGLVKPDALVEIATIAHIAK"
     gene            276..1076
                     /gene="rutD"
                     /locus_tag="ACHURS_003892"
     CDS             276..1076
                     /gene="rutD"
                     /locus_tag="ACHURS_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFR1239509.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPC
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            1086..1676
                     /locus_tag="ACHURS_003893"
     CDS             1086..1676
                     /locus_tag="ACHURS_003893"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFR1239510.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYAPIKWGP
                     TSANCSPARIVFIRTVEGKERLHPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            1687..2181
                     /gene="rutF"
                     /locus_tag="ACHURS_003894"
     CDS             1687..2181
                     /gene="rutF"
                     /locus_tag="ACHURS_003894"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFR1239511.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPSGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            2202..3530
                     /gene="rutG"
                     /locus_tag="ACHURS_003895"
     CDS             2202..3530
                     /gene="rutG"
                     /locus_tag="ACHURS_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFR1239512.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNSQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(3788..3961)
                     /locus_tag="ACHURS_003896"
     CDS             complement(3788..3961)
                     /locus_tag="ACHURS_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFR1239513.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            4334..4930
                     /gene="wrbA"
                     /locus_tag="ACHURS_003897"
     CDS             4334..4930
                     /gene="wrbA"
                     /locus_tag="ACHURS_003897"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFR1239514.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            4951..5178
                     /locus_tag="ACHURS_003898"
     CDS             4951..5178
                     /locus_tag="ACHURS_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFR1239515.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKADKDSLFWGEQTIERKNV"
     gene            complement(5216..6457)
                     /gene="agp"
                     /locus_tag="ACHURS_003899"
     CDS             complement(5216..6457)
                     /gene="agp"
                     /locus_tag="ACHURS_003899"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFR1239516.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(6751..>7142)
                     /locus_tag="ACHURS_003900"
     CDS             complement(6751..>7142)
                     /locus_tag="ACHURS_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239517.1"
                     /translation="SFLSFKKEFIDSCLNDKKCILHLPEFIFNDNKARLVLDTDPEQK
                     VNEVKNFLTALSDSICSLFIVYSNVASISLGNESFSTDDDLEYGYLINTGNHYDVYLP
                     PELFAQAYELNNKERNAQIDFLTRYAI"
BASE COUNT         1625 a         1806 c         1895 g         1816 t
ORIGIN      
        1 taacgtgctg tttgccgatg acccaaaggc gcaaacccgc cacgttctgg aaactatccg
       61 caaggtgatc gagacggcgg gtggcacgat ggcggatgtg accttcaaca gcatctttat
      121 taccgactgg aaaaattacg ccgcgattaa cgaaatctac gccgagtttt ttccgggtga
      181 taaaccggcg cgattctgca ttcagtgcgg actggtaaaa cctgacgcgc tggtggaaat
      241 cgccacaatt gcgcatatcg ccaagtgagg ccgcgatgaa actttcactc tcacctcccc
      301 cttatgctga tgcgcccgta gtggtgttga tttcgggtct tggcggtagc ggcagttact
      361 ggttaccgca actggcggtg ctggagcagg agtatcaggt agtctgttac gaccagcgcg
      421 gcaccggcaa taatcccgac acgctggcag aagattacag tatcgcccag atggcagcgg
      481 aactgcatca ggcgctggta gccgcaggga ttgagcatta cgcggtggtc ggccatgcgc
      541 tcggtgcgct ggtgggaatg cagctggcgc tggattatcc cgcgtcggta actgtgctga
      601 tcagcgttaa cggctggcta cgaataaacg cccatacgcg ccgctgtttt caggttcgcg
      661 aacgattact gtatagcggc ggcgcgcagg catgggtgga agcgcagccg ttgttcctct
      721 atcccgccga ctggatggcg gcccgcgcac cttgccttga ggcagaagac gcgctggcac
      781 tggcgcattt tcagggcaaa aataatttac tgcgtcgact taacgccctc aaacgcgctg
      841 actttagtca ccatgcggat cgcatccgct gcccggtgca aatcatctgc gccagtgatg
      901 atctgctggt gccatcagca tgttccagtg agcttcatgc cgccctgccc gatagccaga
      961 aaatggtgat gcgctatggc ggacacgcct gcaacgtgac cgaccccgaa acgtttaatg
     1021 ctctgttact caacgggctt gccagcctgt tacatcaccg tgaagccgcc ctgtaaggaa
     1081 ttgctatgaa cgaagccgtt agcccaggtg cgcttagcac cctgttcacc gatgcccgca
     1141 ctcacaacgg ctggcgggag acacccgtca gcgatgagac gttacgggag ctttatgccc
     1201 cgataaaatg ggggccgaca tcagctaact gttctccggc acggatcgtg tttatccgca
     1261 ctgtagaagg aaaagaacgt ctgcacccgg cgctttccag cggcaatcta caaaaaaccc
     1321 tgaccgcgcc cgtcaccgct atcgttgcct gggacagtga attttatgaa cggttaccac
     1381 tactgtttcc ccacggtgat gcccgcagtt ggtttacctc cagcccacaa cttgccgaag
     1441 aaacagcgtt tcgcaacagc tccatgcagg cggcctatct gatcgtcgct tgccgtgcgc
     1501 tgggactgga taccggcccg atgtcgggtt ttgaccgtca acacgttgac gacgcctttt
     1561 ttgcgggcag cacgctgaag agcaatctgc tgattaatat cggctatggc gatagcagca
     1621 aactttttgc gcgcctgcca cgtctgtcct ttgaagaagc ctgcgggctg ttgtaaggag
     1681 caaactatga acatagttga tcaacaaacc tttcgcgatg cgatgtcctg tatgggcgcg
     1741 gcggtcaata tcatcaccac ggacggtcca tccgggcgcg ccgggttcac cgccagcgcc
     1801 gtctgcagcg tgaccgacac gccgcccaca ttactggtgt gcctgaatcg tggggcgtcc
     1861 gtctggccgg tattcaatga aaatcgaacg ctgtgtgtaa atacactgag cgccgggcag
     1921 gagccgcttt caaatctttt tggcggcaaa acgcccatgg aacaccgctt tgccgccgcc
     1981 cgctggcaga cgggtgtgac cggatgcccg caactggaag aggctctggt ttcgtttgac
     2041 tgccgcatca gccaggtggt gagcgtcggc acccacgaca ttctgttttg cgccatcgaa
     2101 gcgattcatc gtcacaccac accctacggg ctggtgtggt tcgatcgcag ttatcacgcg
     2161 ctgatgcgcc ctgcttgtta acctccttaa ggagacagct gatggcaatg ttcggttttc
     2221 ctcactggca gttaaaatcg acctctacag agagcggcgt ggtcgcgccg gatgaacgat
     2281 tgccgtttgc tcagacggca gtaatgggcg ttcaacacgc ggtggcgatg tttggcgcga
     2341 cggtgttaat gccgattctg atggggctgg atcccaatct ttccatttta atgtcgggga
     2401 tcggcacgtt actgtttttc tttattaccg gcgggcgcgt ccccagttat ctcggctcca
     2461 gcgcggcttt tgtcggcgtg gtgattgccg cgacgggctt taacggtcag ggcattaacc
     2521 cgaatatcag catcgcactg ggcgggatta tcgcctgtgg gctggtttat accgtcattg
     2581 gcctggtggt gatgaaaatt ggtacacgct ggattgaacg actaatgccg ccggtggtga
     2641 cgggtgcggt ggtgatggcg attggcctga acttagcgcc tatcgcagtg aaaagtgtat
     2701 cggcttcggc ctttgatagc tggatggcgg taatgacggt gctgtgtatt gggctggtgg
     2761 cggtgtttac ccgtggcatg atccagcggt tattgattct cgtaggttta attgtcgcct
     2821 gtctgctgta tggcgtgatg accaacgttt tagggctggg aaaagccgtc gattttactc
     2881 tcgtcagcca tgctgcctgg ttcggtttgc cacatttttc cactcctgct tttaatagtc
     2941 aggcaatgat gctgattgcg cccgtggcgg tgattctggt ggcggaaaac ttaggtcatc
     3001 tcaaggcggt cgccgggatg accggacgca atatggaccc gtatatgggg cgagcattcg
     3061 tcggtgatgg tctggcaacg atgctttccg gctctgtcgg cggtagcggc gtcaccacct
     3121 atgctgaaaa tatcggtgtg atggccgtga caaaagtcta ctcgacgctg gtgtttgttg
     3181 ctgctgccgt catcgccatg ctgttgggct tttcgccgaa atttggcgcg ctgatccata
     3241 ccattccggc cgcggtgatt ggtggtgcat caattgttgt gttcgggctg attgccgtcg
     3301 caggcgcaag gatctgggta caaaaccgtg tcgatttaag ccagaacggt aatttgatta
     3361 tggtcgcagt aaccttagtg ctgggcgcgg gtgattttgc tctcacgctg ggcggtttta
     3421 cgttgggagg gatcggtaca gccacctttg gcgcgatttt actcaatgcg ttgttaagta
     3481 gaaggttggt tgacgttcca ccgccagaag ttgttcatca ggagccataa acagaaaacc
     3541 gccggggtta cccggcggtg cagattgttg acaacgtacg cattgttcat gccggatgcg
     3601 gcgtaaacgc cttatccggc ctacaaaaca gtgcaaattc aatatattgc aatcttctgg
     3661 taggcctgat aagcatcgcg catcaggcaa gcttacattt gtcatcagta tcaaccaccg
     3721 ggtaaccggc ggtgatggct ttttgacgag ctacagacaa aaatgcattc gtcattgatt
     3781 agcccggcta gttgtcgctt ttgccgtgac tgctcttgcc acctttttta cctgcttcag
     3841 atgcgcgctg cgggtcattc ttgaaattac ccccgctgtg ctgtccacct tttttacctg
     3901 cttctgatgc tctttcgcgg tcttctgcaa aattgccgga accgcctcga tggtttgcca
     3961 ttactgacct ccgtctgggt atagacatca tattgcatag aattaacgcg gcgtatggcg
     4021 gctgccatat tttcgctacg tgggactaag cttagtgaac tgacgcgggt agacccgcgt
     4081 ttggtaatat cctgcaacag cttcttagcg agaatagata tcatctccaa taattatcca
     4141 taagccgctc ttatgattaa aaaaacgcga tgagataaaa atgtgtcatt ttgcgacaaa
     4201 attacgtgct tgtgaaagtt gttataaatc aaataagtgg ttgtgaaatt tgcactctga
     4261 aaaggacgtc ttatctttaa ataagtggta gcgaatcgct acggaataga gataacacga
     4321 ggagtggtta gaaatggcta aagttctggt gctttattat tccatgtacg gacatattga
     4381 aacgatggca cgcgcagtcg ctgagggtgc aagcaaagtc gatggcgcag aagttgtcgt
     4441 taagcgtgta ccggaaacca tgccgccgca attatttgaa aaagcaggcg gtaaaacgca
     4501 aactgcaccg gttgcaaccc cgcaagaact ggccgattac gacgccatta tttttggtac
     4561 acctacccgc tttggcaaca tgtccggtca aatgcgtacc ttcctcgacc agacgggcgg
     4621 cctgtgggct tccggcgcac tatacggaaa actggcgagc gtctttagtt ccaccggtac
     4681 tggcggcggt caggaacaaa ctattacttc aacctggacg acccttgcgc atcacggcat
     4741 ggtgattgtc cccattggct acgcagcgca ggaattattt gacgtttcac aggttcgcgg
     4801 cggtacgccg tacggcgcaa ccaccatcgc aggcggtgac ggctcacgcc agccaagcca
     4861 ggaagaactg tctattgctc gttatcaagg ggaatatgtc gcaggtctgg cagttaaact
     4921 taacggctaa tcttcaacag gaggatacgc atgccaactc aagaagcgaa agctcatcac
     4981 gtcggtgaat gggcatctct gcgcaatacg tcgccggaaa tagccgaggc cattttcgaa
     5041 gtcgccgggt acgacgaaaa aatggcagaa aagatttggg aagaaggtag cgatgaagtg
     5101 ttagtcaaag cctttgctaa ggccgataaa gattcgcttt tttggggcga acagaccatc
     5161 gaacgtaaaa acgtttaacc agcgactccc ccgcttctcg cgggggagtt ttctgttatt
     5221 tcaccgcttc attcaacacg ctatcaaatt tatccatcgg gcagaaaccg ttggcatcta
     5281 tcgggcaacc gcttaattcc agcgtcacac gctgagcagg tgcctgcagg gttaacgcat
     5341 cggcattacg taactgttcc gcactctgat acacatattc aattttcatc aaatcgcgat
     5401 tggctttgct gtcatgccaa cgctggaaaa cgattttgcc gccaatcggc gtgcgttcgt
     5461 tctggtcatg caactgatac ggtttgaaat ccagcgccgt taacagagag gcaatgttgg
     5521 agtcgtgccc aaccaacact gtaattttcg gtgcgctggt gcgatcggtg accagagctt
     5581 tgtcgatata actgaccagc ggtttcgcaa cattgcgcgc cacttccggt gaggtaaaca
     5641 ggctgtcctg gtagccgttt ttaagcttcg acaacacctt ccactgctgg tcagatttga
     5701 tttctcccca ggccacctga tccatcggaa aaccttcgta atattgcaaa gtaaacgcat
     5761 ctaccagcga gttgccgact ttcagcggcc cggaaacacc tggttcttgt tgatacttgg
     5821 cgctaaaggt atttttgcca tccaccagcg aacactgttg tttctcttta caggcagggg
     5881 aatctttata gttaacgatt ttttccagta gctggtagct gtcggtaagc tggagtttgc
     5941 tgagctcttt ctccattgcc gccaccgcct gttcactgaa tgcggcggaa tcatcggtga
     6001 tcaccgggtt aaaggttggg tccatagtgc ccattttttc ctggtgatgc acaggaatat
     6061 cacacccggg gaatgcgccg gtaataaaga actgtgcggt cgcaacggta cgttgcagac
     6121 tattggcata ggcataaacg gtgtccggcg gcgggcattc ccccgatttc accatcccct
     6181 gctctgccag ccattcacgc atgtaatggc ccatatacac ttcgagcacg ccacctttgg
     6241 tggtgagttg cccaccgggg acgtcccatt ctggccattt attcggcgtc gactgctcca
     6301 gcacactgcc attgttcgcc agcggcgcac gtaagttatg gcggctcatc atgagcactt
     6361 gctgtagctg atagccttcc ggtacggttt gtgcctgagc atttgaagcg agtaaaacta
     6421 tccctgccac agttgcggcg attagcgttt tgttcattcc tggcacctct tttgttatca
     6481 atcattaaag agtgtgacag aaatatgact tttttccggg aacaggttcg caaaatggct
     6541 tgttgaacgc ctgtttttct atatatatct tcaaaaacag gcaaggtttg acgcaaacta
     6601 tgcctgatta gcccctgcct cgcgtacgcc gctgataatt gcacctgtgg ctaaacagaa
     6661 tatcagcggc gttactaata cagaggcaat agtaccaata ataagagaag tgaggctaat
     6721 aacaatggtt tttagcacat gcctttgtca ttaaattgca taacgagtta aaaaatcaat
     6781 ttgcgcattc ctttccttat tgtttaactc gtaagcctga gcaaaaagtt caggagggag
     6841 gtaaacgtca taatgattgc cagtgtttat taaataccca tactcaaggt cgtcatctgt
     6901 tgaaaaggat tcattcccta aggagattga tgccacattg ctgtaaacaa taaataatga
     6961 gcaaatgcta tctgaaagag cagtgaggaa atttttcacc tcattaacct tctgctctgg
     7021 gtcggtgtct aaaacaaggc gtgccttgtt atcattaaat ataaattcgg gtaaatgcag
     7081 tatgcatttt ttgtcattca agcaactatc aataaattct tttttaaaag acaaaaaact
     7141 cc
//
LOCUS       DAZPDU010000204         7122 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZPDU010000204 DAZPDU010000000
VERSION     DAZPDU010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7122
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.206"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..436
                     /locus_tag="ACHURS_003901"
     CDS             <1..436
                     /locus_tag="ACHURS_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFR1239518.1"
                     /translation="KVALNLASGEGNFFIRPGGVFYVAGDKVDIVRLDAFKTSKEIQF
                     AVQSGPMLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAK
                     AKLNVEQLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            519..1421
                     /locus_tag="ACHURS_003902"
     CDS             519..1421
                     /locus_tag="ACHURS_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HFR1239519.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVRQIDEKTNRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(1476..3638)
                     /gene="uvrD"
                     /locus_tag="ACHURS_003903"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(1476..3638)
                     /gene="uvrD"
                     /locus_tag="ACHURS_003903"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFR1239520.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(3722..4438)
                     /gene="yigB"
                     /locus_tag="ACHURS_003904"
     CDS             complement(3722..4438)
                     /gene="yigB"
                     /locus_tag="ACHURS_003904"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFR1239521.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(4438..5334)
                     /gene="xerC"
                     /locus_tag="ACHURS_003905"
     CDS             complement(4438..5334)
                     /gene="xerC"
                     /locus_tag="ACHURS_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFR1239522.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENV
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(5331..6038)
                     /locus_tag="ACHURS_003906"
     CDS             complement(5331..6038)
                     /locus_tag="ACHURS_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HFR1239523.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(6035..6859)
                     /gene="dapF"
                     /locus_tag="ACHURS_003907"
     CDS             complement(6035..6859)
                     /gene="dapF"
                     /locus_tag="ACHURS_003907"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFR1239524.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6896..7099)
                     /locus_tag="ACHURS_003908"
     CDS             complement(6896..7099)
                     /locus_tag="ACHURS_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR1239525.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
BASE COUNT         1639 a         1990 c         1856 g         1637 t
ORIGIN      
        1 gaaggtggcg ttaaatctcg cttcaggtga agggaatttc tttatccgtc ctggcggcgt
       61 gttttatgtc gcgggagata aagtcgacat cgttcgtctg gatgccttca aaaccagtaa
      121 agagattcag tttgcggtgc agtcagggcc aatgttgatg gaaaacagtg taattaatcc
      181 gcgtattcat cccaacgtcg cctcacgtaa aattcgtaac ggtgttggga ttaataaaca
      241 tgggaatgcc gtgtttttgt tgagccagca ggcgacaaat ttttatgatt ttgcctgtta
      301 tgccaaagcg aaactgaacg ttgagcagct gctttatctt gatggcacga tttcacatat
      361 gtatatgaaa ggcggggcaa tcccgtggca acgctatcct tttgtcacca tgatttccgt
      421 cgagaggaaa ggttaagcat tgtacactcg aggaggccag acaaactggg gctggctaca
      481 cagctgaaaa atcatcagat aaatgaaggg aaacaaaaat gaaaaaaacg ctcgcaacgt
      541 tatttttatt aacgtgtctg ggatcatcat ccgcttatgc ggataacgct ttgattttac
      601 aaactgattt tagcctgaaa gatggtgccg tctcggcgat gaaaggggtc gcattcggtg
      661 ttgaccataa tcttaaaata tttgatttaa cacacgaaat tccaccgtat aacatctggg
      721 aaggcgctta ccgcctgtat cagaccgcca gttactggcc gcaaggttcg gtatttgtca
      781 gtgtggtcga tcctggtgtt ggtacagacc gtaaatcggt agtgctaaaa accaaaaacg
      841 gtcaatactt cgtttcacca gataacggta cgctcacttt ggtggcggaa tcgctgggta
      901 ttgagagcgt ccggcaaatc gatgaaaaaa ccaaccgcct gaaaggttcc gaaaagtcat
      961 ataccttcca cggtcgtgac gtgtatgcct acaccggtgc acgcctggct tccggagcga
     1021 tcacgttcga acaggtgggg ccagagcttc ctgcaaaagt tgtcgaactt tcttatcaga
     1081 aagccaaagc aacaaaaggt gaagtgaagg gcaatatccc gattctcgat attcagtacg
     1141 gcaacgtctg gagcaatatc agcgacgagt tgctgaatca ggcaggaatc aaactcaacg
     1201 acacgttgtg tgtaaccatt tccgaaggtt cccagcaaaa atacgcaggg aaaatgccgt
     1261 atgtcgcaag cttcggcgat gtgccggaag gccagccgat ggtgtatcta aacagcttat
     1321 tgaatgtttc cgtggcgctc aatatggata atttcgcgca gaaacatcaa gttgcgtctg
     1381 gtgctgactg gaatattgat gtgaagaagt gcgctaagta aggtgctgat gcaggatgcg
     1441 gtgtggcgca ccgcatcctg caacggagtt gtaaattaca ccgtctccag ccgggcgtaa
     1501 gccgccacca gccatttgat tccctggccc tggaatgcca cctgcaaacg gctatgttca
     1561 ccgctgcctt ccatattgac gatggtgcct tcaccaaact tagcgtggcg cacgcgctgg
     1621 ccgagtttgt agccgctgtc gttctcgacc atcggcgtac ccatccgctg atggctaacc
     1681 gggcggctta ccgtggcgcg caggcgcacc tcttccacac actcttccgg cagctcgccg
     1741 ataaagcgcg acgggcgatg gtaaacctct ttgccataca gacggcgagt ttccgcgtag
     1801 gtcagcgtca gtttctgcat cgcacgggtt acgccaacgt aggccagacg gcgttcttct
     1861 tccagacgtc cgccttcatc cagcgacatc tggcttggga acatgccctc ttccataccg
     1921 acgataaaca cctgcgggaa ctccagccct ttcgccgagt gtagcgtcat caactgcacc
     1981 gcatcctgcc aagtatccgc ctgcccttca cctgcttcca gtgccgcatg ggagaggaac
     2041 gcctgcagcg gcattaaatc ttcgtcttct tcgttgtagc tgaactggcg cgttgccgtc
     2101 accagttcct ctaagttttc gatacgcgtc tgaccttttt cgcccttctc ctgctcgtac
     2161 atggtacgca ggccggagtc tttaattacc cggtcagtct gtacatgcag cggcatatcg
     2221 gcagtttctt gcgctaaggc atcgattaat tccataaatc gctgcaaggc gctggcagct
     2281 cgcccggcga gggctttttc ctgcaacagt tcacgacatg cctgccagag tgttaactgg
     2341 cgatcgcgcg atgtctgacg taccacgtcc agcgtccggt caccaatacc ccgcgttggc
     2401 gtattcacca cgcgctcaaa cgccgcgtcg tcgttacggt tggcaatcag acgcagatac
     2461 gagagcgcgt ctttgatttc ctggcgttcg aagaagcgca tcccgccgta aatacggtac
     2521 ggcatactgg cctgcaacag tgcttcttcc agtacacgcg actgggcgtt actgcggtag
     2581 agaatggcgc actcggcaag cgccccgccg ttgtcctgcc aggttttgat gcggttaacc
     2641 acaaaacgcg cttcgtcgag ttcgttaaaa gcgcaataga gggaaatagg ctcaccgtcc
     2701 gcgccatcgg tccacagttt tttacccaga cgcccgttgt tgttttcaat cagggcgttc
     2761 gcggcgctca gaatattgct ggtagagcgg tagttctgct ccagacggat agtttcagca
     2821 ccggggaaat cattgaggaa acgctgaata ttctctacct gcgccccgcg ccagccgtag
     2881 attgactggt cgtcatcgcc aacgatcatc actttgccgg tatcgcccgc cagcaggcgg
     2941 atccacgcgt actgaatgtt gttggtatcc tggaattcgt ccaccaggat gttggtaaaa
     3001 cgttcgcggt agtgttgcag gatatgcggc ttgttaagcc acaactcgtg agcgcgcagc
     3061 agcagctcgg cgaagtccac caggcccgca cgatcgcacg cttcctgata cgcctgatac
     3121 accttctgcc aggtctgctc caccggatta ccgtagcttt gaatatgatg cggacgcagg
     3181 ccttcatctt tctggctgtt gatgtaccac attgcctggc gcggcggcca ctgcttctcg
     3241 tcgaggttca tggctttgat cagacgctta agcaggcgta gctggtcttc actgtcgagg
     3301 atctggaaat cctgcggtag attggcgtcc atatggtgcg cacgcagcaa acggtgcgcc
     3361 agcccgtgga aggtgccgac ccacatgccg ccctggctgg tgcccatcag ttgcccgata
     3421 cgatgacgca tctccgccgc cgctttgttg gtaaacgtca cagccataat cgagtatggc
     3481 gagcagtttt ccacgctcat cagccaggcg atacgatgca ccagtacgcg cgtcttacca
     3541 ctgcccgcgc ccgccagcac cagaaggttg ctgcgtggcg cggccaccgc ttcgcgctgt
     3601 ttgtcattaa ggctgtcgag caggtaagaa acgtccattg gcaccgccgc gtaaaaatag
     3661 gttgggcgga gaagcgcaac gccctccgcc aaaaagctgg gtatatatac agatttactg
     3721 attatatcag cgaggtcaga gatgccaacc gggaaatttc cagatgcggc agtaaacggc
     3781 tgtcccaggt ttgcatcaga tcgccatttt ccggtctgat ccaacaagcc tgcattccgc
     3841 tgcgaattgc cccacccacg tcagtggtga gatcgtcccc aacatgtaag atctcgccga
     3901 tcggcacgtt gagtttttcc gcagccaaaa agtacatatc gctgaacggt tttgagcgcc
     3961 cgtgcgggcc agcgcgcagc acaaactcaa aataatcccc caaaccaaac agttccggct
     4021 gggcgttacc gttggtgatc gccaccagcg gccatttctt cgccagctgt tttaaggtgt
     4081 cgtgagtttg ctgcgggacg tcgattcggc tgcgccattt ggcaaagtta atcattgctg
     4141 cgtgtgcgcc tgcactggct tcttcggcac tcagcccggc gtcgagcatc gcttgttcaa
     4201 tcgaacgaaa acgccaacgc gtcacgtcgt gataaatctc gggttccgct tcccgtaccg
     4261 cctggcgcag gcgttgcaga tcttcattct ggaagctgcg cagcgccgga tgataatttt
     4321 gcacaaaggt aagcgcctct cgctcggtgc gcaaaatcac cggacggtta tcgtaaaggg
     4381 tatcatccag gtcaaaggtg agcgccgaga tgcgccccaa aggccggtaa aaacgcatta
     4441 tttcccccgt ttggcgcgtg gatgcgccgc atcgtacacc gaggcaaggt gttgaaaatc
     4501 aagatgagta tagatttgcg tggtggagag gttggcatga cccagcagct cctgcacacc
     4561 acgaagatcg ccgctcgact ccagcatatg cgtggcgaac gagtgacgta atttatgcgg
     4621 atgaacgtga ttattcagcc cttgttttat gccccattcg gcaaagcgtt tctgcacatt
     4681 acgcgcggag atacgcttgc ccagtttcga cagaaaaagc gcgtcgtctt cgctaccaaa
     4741 caggtcgcgc aaatcaagcc agtgctcaat ccacgccaca gcgttgcgac caatcggcag
     4801 gcggcgctct ttgctgcctt tccccattac ccacacttca ccagactcca ggtcgaggtg
     4861 tttgatatcc agccccacca gctcggaaag acgcagaccc gcgccgtaca tcacttccag
     4921 cattgcacgg tcgcgtacag cgaggggatc gttgatatca atgtccagca gccgattcat
     4981 atcgtcgacg tcgatatttt tcggcagatg acgcggcgct ttcggtgccg aaacaccttt
     5041 cgccgggtta gctttgagtt cgttctggct gaccagccag tcaaaaaagc tacgtagcgc
     5101 agaaagccgt aacgcgaggc ttgctgctcc cagcccttta cggcgactgc gtacagcaaa
     5161 attgcgcacc atcgtcacat cacattgctg ccagctttgc aggacgtttt cgctggcaaa
     5221 attgatgatc gcctcaagct gacgctggta gttaagcagg gttatcgggc taagctggcg
     5281 ctccacgctc agataacgta ggtagcgttc tacatcggtg tgtaaatcgg tcatacgcgt
     5341 tcaatccaac gctccagaag ctccggcaac atcagcgcaa tttcatgaag taactgcgtt
     5401 ccctgccctt gttgatagtg actggcatcg cgactggtaa acagcacgac acccaaatca
     5461 gcatcgcttc ccagcatcga catcgccacc gatcccaccg ctttcgcttc cggtagcacc
     5521 accagcagct ctggtccgtt aagcggccca agatagtgct gttcctgccc caaacgctga
     5581 atacgcagcg gttcgaaaga ctgacggctt aatgccagat gagtgtggtt cgacggcgca
     5641 cctaagcgcc agcgatccgg aaacaggcgc agactcgcac ctgccaggcc gagatcgcgc
     5701 gcccagcggt gaaagcgcat cagcatatcg tcgagactgc tggcggcggt gagactgcgc
     5761 tgcaggtaga gtaggcgata aaacaggcct tcgttggcga tagcctgttc catcaacagc
     5821 gccatgttct cttccagaac atgaatatga ttacgtgcgc gggccatgtg ccactcgacc
     5881 aacgaaacgg tgccgcgtac cggatgcggc acacgtatcg cttctactgc gcgcgcatta
     5941 cggataaaaa actcaggatt tttaatcaga taatcgacaa ccgcccggtc atcaagctcc
     6001 gtgagtgttt cctgcagttc ttcccctggt tgcttcatag atgaataaat ccgtcgtaga
     6061 catgtaccgc cgggccagtc atatataacg ggtgacccgg acctttccag gcgatatcaa
     6121 gacgaccgcc ggggagttcc acgcgtactt cttcggccag caaaccttgc tgaatcccta
     6181 ctgcaaccgc cgcacacgcg ccgctgccgc aggcctgggt ttctcctgcc ccacgctcat
     6241 aaacgcgtaa acgaatatgc tcgcgcttaa ccacttgcat aaaaccgata ttggcgcgct
     6301 ccggaaaacg ctcgtggctt tccagaacag gaccaagcgt ttctaccgcc gcggtatcga
     6361 catcatcgac ctgaatcacg caatgcggat ttcccatcga caccacgccg cataagattg
     6421 tctgctcggc ggcgcgcata atataggtct tttccgcttt gttagcgcga aacggcacgg
     6481 cggaaggttc gaagttgggt tcgcccatat ttacgcggac cagatcatca tcggtgacgg
     6541 tcagaaccat ccgcccgttg gcggtgctga cgcggatatc acgcttattg gtcagtcctt
     6601 tcagacgcac aaaacgggca aagcagcgcg caccgttgcc gcactgcgcc acttcactgc
     6661 catcagcatt gaaaatgcga tagtgaaaat ccagttcagg atcatacggc ggctcaacca
     6721 ccagcagttg gtcaaaccct acccccaggt gccgatcagc caggcgacga atcagctccg
     6781 gtgaaaaaaa gacattctgc gttaccgcgt cgacgaccat aaaatcgttg ccaaggccat
     6841 gcattttcga gaactgcatc atttactcca atcacgcggg tacagaaact gacttttagt
     6901 aattcacctg ggatggacca tcgccagtgg cgcgatcgtt tttatccggc accgtggatt
     6961 gcgtttgcgt ctctaccggt ttggtcggcg gcggtgcgtt tttatctgca ggcgggaaat
     7021 agagcggacc tttcagaccg cagcccgtca ggctgaagag agtaagtaat acagtgagtg
     7081 ccttaaacac gtttttcatt atggattgcc tgtaaagttc at
//
LOCUS       DAZPDU010000205         7109 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZPDU010000205 DAZPDU010000000
VERSION     DAZPDU010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7109
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.207"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..350
                     /locus_tag="ACHURS_003909"
     CDS             <1..350
                     /locus_tag="ACHURS_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR1239526.1"
                     /translation="RELKLANATITDMQQRQRDADALDAKYTKELADAKAENDALRRK
                     LDNGGRVLVKGKCPVPSSAETSSASGMGNDATVELSPVAGRNVLGIRDGIISDQTALR
                     MLQEYIRIQCLGG"
     gene            complement(439..951)
                     /locus_tag="ACHURS_003910"
     CDS             complement(439..951)
                     /locus_tag="ACHURS_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFR1239527.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            1146..1910
                     /locus_tag="ACHURS_003911"
     CDS             1146..1910
                     /locus_tag="ACHURS_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239528.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLGWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1861..3264
                     /locus_tag="ACHURS_003912"
     CDS             1861..3264
                     /locus_tag="ACHURS_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFR1239529.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDVLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAAIIKLSWIEAAVDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLVADRFRNTFNAINNGEQYPVD
                     ELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAKREIPSPNVADAFIMAF
                     APIDTSLDIWEQLGRQA"
     gene            3264..4730
                     /locus_tag="ACHURS_003913"
                     /pseudo
     CDS             3264..4730
                     /locus_tag="ACHURS_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
     gene            4621..5364
                     /locus_tag="ACHURS_003914"
     CDS             4621..5364
                     /locus_tag="ACHURS_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HFR1239530.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            5368..6588
                     /locus_tag="ACHURS_003915"
     CDS             5368..6588
                     /locus_tag="ACHURS_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HFR1239531.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFGEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKELAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            6592..7098
                     /locus_tag="ACHURS_003916"
     CDS             6592..7098
                     /locus_tag="ACHURS_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239532.1"
                     /translation="MNNVFLYRMPVGIAGAVSRPQDLTVEPVVLKSDNAFAAYGLAGK
                     YDDDGFFVPLADGDTADKVKGIYVRPYPTTSQPDMVRQVGTGKNFPGDAMKRGYVTVN
                     LGSDFDASTIKKGDPVYVVVSTDESIKVPLGGFMSTSVSGKNVVLTNAEFTGAGDADG
                     NAEISWKI"
BASE COUNT         1889 a         1698 c         1946 g         1576 t
ORIGIN      
        1 caagagaact gaagctggcg aacgcaacca ttactgacat gcagcagcgc cagcgtgatg
       61 ctgatgcact cgatgctaaa tacacgaagg agttagctga tgcgaaagct gaaaatgatg
      121 ctcttcggcg caagcttgat aatggtggtc gggtgctcgt caaaggaaaa tgccctgtgc
      181 catcctcagc cgaaacctcc agcgcctccg gcatgggcaa tgatgccacc gtcgaactct
      241 ctccagttgc tggacgaaac gttctcggta tccgggacgg aatcatcagc gaccagacag
      301 cattgagaat gcttcaggag tacatcagga tccaatgcct tggggggtag cggtaatttt
      361 actcattatc cttcaaatca gattctgttg tcagaggaat gggggaagct ggattcgcaa
      421 gagcttttta tgggcagttt agctaaaaaa aggcaacacg aagctaaaac taccaacata
      481 aagtgctgac cagctagcag ctgaaatcag actcactgta tacactttca ctggatgaag
      541 ttgcaacatt cctgccacca aaggagcaat ataacgcagc acggcgataa agcgcgaagt
      601 gaaaagtatt gacacagagt tattttgcaa ctgaaggcga acccgttcta ttgtattgga
      661 acggcttgat attatacatg caagttgagg gatatgccca attattatac caagatggta
      721 attaacgata gtaccgcacc atgcgccact catgaccgtg attccagctt cccatggtga
      781 aagagtcgtc ttgctgactg taattacagt ggccatcata attgatgctg gtggcaatat
      841 cgccgaaatg agtaaagtgg attttgtgaa cgctattata aaaagaagcc cccataggcg
      901 atatggatga agtacaaaat agttcatcaa agcatttatc cactccatca atgtctttcc
      961 ttctgtgaat ttttctttgc tatttaaccg tgatacgact taatttaggc ttaaacacct
     1021 tacatttaca aggtactcct gtggggggcc ctgccacgga gcgtcgcagg agtgagtgat
     1081 gaggaagaat taaagcaacc tattgtgaca ggcagcgata acagtaaatc acaggaagca
     1141 taatcatggc aaaaccggac tgggaggcca tcgaatcggc ataccgggcc ggagtcctta
     1201 gcctccgtga tataggcgag aaatacggcg ttacagaagg ggctatcagg aagagggcca
     1261 aaaagcttgg ttgggcacgc agtggcggta cgcaggtttg caaaaatggt acgcaaaaaa
     1321 ggaaagtgcg taccagcaga aagcctgcca ttactggcct tacacaaaaa agtacgcaac
     1381 taaaaacaga atctacaccg gatacgaaac cgatacgcgg aatgcgtacc gatcccccga
     1441 ctaacccatt ccaacccggt aaccagcagg cattaaaaca cggtggttat gcccgtcgcc
     1501 ttctgctcaa agatgaggtg atagaggacg ctaaagcgtt gacgctcgag gacgaattat
     1561 ttcgccttcg tgctaacaac cttgtcgctg cagagaatat tggtcggtgg ttggtgtcgc
     1621 tggaagatgc taatggggac caggaaagga agatgctgat tgaaaatatc agcgccgccg
     1681 agaaagcaat gatgcgcaat acagttcgta ttgagtccat cgttggcacg cttgcgacgg
     1741 taggaaaaat atttgctgat acagcctacc gcaaggccgc cactgataag gtgtctctgg
     1801 aggctgatcg tcttcgccgt gatgcaggta ttgatgatgg caatggagag cgtgacctca
     1861 atgacttcta ctctgacatc caaaccgacg ctgaatccgg ctttacgtag tttctggact
     1921 atgcgggcac gtaacaaagt gctttatggt ggtcggtcat cgtcaaaatc atgggatgcc
     1981 gctggcattg ccatatttct gtcgaataaa tacaccctgc gtttttgttg tgcccgtcag
     2041 atccagaata aaatcgaaga gtcggtgtat accctgctca aaattcagat agacaggttt
     2101 ggtctgcggc accgtttccg tattctgaac aacaaaatca ttaaccgggt tactggctcg
     2161 gaatttgttt tttatggatt atggcgcaac atcgaagaaa ttaagtcact ggaggggatc
     2221 gatgtgttgt ggctggaaga agcccacgca ctgacggaat accagtggaa aattctggag
     2281 ccaacgatcc gtaaagaggg ttcggaatgc tggttcatat tcaaccccgg acttgttact
     2341 gatttcgtct ggcgcaactt cgttgttgat ccgcccgaag gcactctcat ccgcaaaatt
     2401 aactatgacg aaaatccgtt tctgtctgac accatgctta aggttatcga cgcggcgcga
     2461 cgccgtgatc cggatggttt taaacatgtg tatgagggcg ttccggagtc tgatgatgat
     2521 gcggcaatca tcaaactgtc ttggatagaa gccgcagtgg atgcgcacaa aacgttaaat
     2581 ttcgaaccca gtggaagaaa gcgtattggc tttgacgtgg ctgacagtgg tacagataag
     2641 tgcgctaacg tttaccgtca cggatccgtt gttttctggg ccgacgaatg gaaggccaaa
     2701 gaagatgaat tactgaagag ctgccagcgt acttatcagg cggcgctgga gcgtgaagca
     2761 gatattgttt acgactctat cggtgttggt gcgtctgccg gtgctaaatt ctctgaaatt
     2821 aacgctgacc ggaagagcga gaacgcatac gcgcgacgtg tgaattacca gaggtttaac
     2881 gccggtgctg gtgtgcatga gccagatgac gagtacaacg gcatccccaa caaagacttt
     2941 ttcgcaaatc ttaaggctca ggcatggtgg ctggtggctg accgtttcag aaatacgttt
     3001 aacgccatta acaacggaga acagtatcct gtggatgagc tgatcagcat agattctcgt
     3061 tgtccgttgc ttgaaaagct gaaactggaa ctgacaacac ctcatcgtga tttcgaccgt
     3121 aacggacgtg tgatggtcga aagtaaaaaa gacctcgcaa aacgcgagat accgtcacca
     3181 aacgttgctg atgcattcat tatggccttc gcgccaatcg atacatcgct ggatatctgg
     3241 gaacagctgg ggagacaggc ctgatggcac gaaacaaaca agccctgcgg cgaactgtgc
     3301 aggccacagc tgatggttat gagaatttta ttgcccgcgt agggatgcag acacctaacc
     3361 agcactcagc atccacctac cgggctaatt tcaccagtcg taaccgcatg ctggtggaat
     3421 ggtcctatcg ttcatcctag atcatcggcg aagcagtcga tgctatcccg gatgatatga
     3481 cccgcaaagg cattcgcatc acttcggaaa ttgatgcaaa agatcgtggc attctcgaat
     3541 cacaactgga tgagttgcaa atctgggatg cgctgaatga cgtgctgaaa tggtcgcgcc
     3601 tctacggcgg cgcggtgggt ttcatcatga ttgaggggta ggcaccaatg accccgctgc
     3661 gacccgaaac catcggtaag ggcaagttta aggggattct cccgctcgac cgctggatgg
     3721 tcgacccggc actgacccgc cgcattaaag atatggggcc ggacctgggt aaacctgagt
     3781 tttacgatgt ggtgaccaca gcaacgggaa ttcctgcctg gcgcattcat cacagtcgcc
     3841 tgattcgctt tgatggcgtc acgttgccat ttcagcagaa gatgaccgag aacgaatggg
     3901 gaatgtcggt tgtagagcgt atctgggatc gtcttaccgc gttcgacagc gctactgtcg
     3961 gcgcggcgca gctggtctac aaggcgcatt tgcgtaccta cagcgtggag aagctacgcg
     4021 agcttatcgc acttggtggt cctgcgtatg aagcgttgct gaagaatatc gacctgattc
     4081 gacagttcca gagtaatgaa ggcatgacgc tcatggactc gcgggataag tttgaaaccc
     4141 atcagtacag cttcagtggt ctggatgaca tcctttcgca gtttgtagaa cagattagtg
     4201 gcgctgttgg tatcccactg gtgcggttgt tcggacagtc cccgaaagga ttttctaccg
     4261 gtgatgcaga ccttgccaac tattacgatc gcatcagttc gttgcaggag aggcgtttac
     4321 gtcttccggt gcggcggata ctggacatca tgcatcgttc ggaacttggc aagccgctgc
     4381 cggacgattt cacgtttgag tttaacccgc tctggcaaat gtctgatgtc gatcgctcaa
     4441 cggtggcgtt aaacaccacc aacgcaatca gtacggcgct gggtgatggt ctgatgacac
     4501 tgaaagccgc tatgactgat ttgcgagaaa attctgacgt aaccggcatc ggggcatcca
     4561 ttaccgacga ggacatcgag aatgccgaag atgaagcgcc gcccggcatc ggcgaacctg
     4621 atgacgaacc gcaggagccg tcaggcggaa atccggtatc gaaccagcct acgcaggata
     4681 gcgagggcgg tcggagacat cgtaaatggt cgctacgatg gttcaaatga cagtatcacg
     4741 gaaattattg cggcgctgga gcgttacagt gaaatcatca ccccctgggc gacaaaggtc
     4801 gcggaaaact ttactgcgga cctaacccgg cagaacgaga aagtttggcg gcaacacagc
     4861 aagaacatca gtcgcgagct ccgcaatctt gtggaaagcg ctcctgtggg ccaggtgatg
     4921 caatccatca tcgccgaaca ggtcaagtac atcaaatctc tgcctctcga ggccgcagac
     4981 agggtgtacg acatccagaa caaagcgata gaggctgttg tcaccggtgg tcgggcggag
     5041 cagtttgcta aagagattgc atctaccggc gatgttgcta agtctagggc cgatctgatt
     5101 gcccgaacgg aactgggaag agcaacgggc gcgctggata tggcccgagc gatagctatt
     5161 ggctcggatg gttatatttg gcgtacagcc gatgatggcg atgtccgaga ttcccacgat
     5221 cacatgaaag gtaaattcgt ccgctgggat tcacctccaa ctttggacgg catgaccggc
     5281 cacgcgggcg agctgccaaa ctgccgctgc tataaagaga tcgtgtttgt tcgcgttcca
     5341 ttcgcaatga aaagggcagc ataacccatg aaatactttt ttgagaccag gctcggggaa
     5401 acccgatacc gcctggctga cggctcgttg ctgtgcaaag acgtgccgat aggacgaaca
     5461 ggtaagcagc tctatggtgc tgatgacctg ccaaaactga aacccgataa gttcggtgaa
     5521 atagtcgtca cgcgttctcc tgagcaggta ttccatccgg ccacgcttgc ctcattcgaa
     5581 gggatgagca tcacgatcct gcatcctgaa gatgaaaacg ggaatgtgcg gctggtaaat
     5641 cccgagaact ggaaagagct tgctgtcggg cacctccaga atgtccggcg cgggacgggt
     5701 gagcagtctg atttgatgct ggctgacctt atcgtcaaag acgaaagcgc cattcagctt
     5761 atcgaagatg gcctgcgcga agtgtcgtgc ggctatgacg cggagtacga gcagaccgag
     5821 ccaggtaaag ccgagcaggt cgatattacc ggaaaccatg tggctcttgt ccctaaaggc
     5881 agagccggaa atcgttgtgc aattggagac agagacacaa tggcaaatca aaagaaaaac
     5941 tggtggaacc gcatgcgtgc ggccatcaag acaggagatg ccgacaccat gaacgaactg
     6001 gtggagtcgg ctcccgcatc ggttacagga gatgaggggg atttgccgca gggcgttaat
     6061 ctcaacatca acctgtcccc gcagcaacca ctaccggaca aagcaccaga gatgggcgga
     6121 gatccaaccg gcgacagtga tgatgacctc aaaacattac tgaaagccct gctggctaag
     6181 ctggaaagaa atgccacggg cgataacgat aataagcctg acgataatcc gaccggtgac
     6241 ggcgaggacg atgaagagga aaccacgatt actggtgact cagcctggcg tgccgaagtt
     6301 atcgttccgg gtatcgatct gagccgtaag atgaaaccga ccgcgttcaa acgcgaggtt
     6361 ctggcttctg ctgacaaaac gctggttcgc cagatagtcg gtgatgcgga tatccgcaaa
     6421 ttaccgaaac aatcggtcga catggcgttt aatgccgtgt ctgaggttgc caaagggcga
     6481 aacacccgcg ccaccaccgg cgatgcacag cgcctaaaca tgggcatgac cagtatcgct
     6541 tccctgaaca aacaaaacgc tgaattctgg gcaaaccgta aagggtaaaa aatgaataat
     6601 gtatttctgt accggatgcc tgttggcatt gccggggctg tctctcgccc gcaggactta
     6661 accgtcgaac cggtggtcct taaatccgat aacgccttcg ctgcctatgg cctggctggt
     6721 aaatacgatg atgacggttt tttcgtgccg ctggcagatg gtgataccgc agacaaggtg
     6781 aaggggatct acgtgcgccc ttatccgacc acgtcgcagc cggacatggt tcgccaggtg
     6841 ggaacaggca agaacttccc gggcgacgcc atgaagcgtg gctacgtgac cgttaatctc
     6901 ggttctgatt ttgatgccag cactatcaaa aaaggcgatc cggtatacgt tgtcgtctcc
     6961 actgatgaat ccatcaaagt gccgctgggt ggattcatgt ccacgtcagt cagtggcaaa
     7021 aacgtggtgc tgaccaacgc tgaattcaca ggtgccggtg atgctgacgg caatgcagaa
     7081 atttcctgga agatttaagg aacagacga
//
LOCUS       DAZPDU010000206         6998 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZPDU010000206 DAZPDU010000000
VERSION     DAZPDU010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6998
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.208"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            532..1521
                     /gene="araF"
                     /locus_tag="ACHURS_003917"
     CDS             532..1521
                     /gene="araF"
                     /locus_tag="ACHURS_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFR1239533.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDALSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1591..3105
                     /gene="araG"
                     /locus_tag="ACHURS_003918"
     CDS             1591..3105
                     /gene="araG"
                     /locus_tag="ACHURS_003918"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFR1239534.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRSIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3120..4106
                     /gene="araH"
                     /locus_tag="ACHURS_003919"
     CDS             3120..4106
                     /gene="araH"
                     /locus_tag="ACHURS_003919"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFR1239535.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITILATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4273..5073
                     /gene="otsB"
                     /locus_tag="ACHURS_003920"
     CDS             4273..5073
                     /gene="otsB"
                     /locus_tag="ACHURS_003920"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFR1239536.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            5048..6472
                     /gene="otsA"
                     /locus_tag="ACHURS_003921"
     CDS             5048..6472
                     /gene="otsA"
                     /locus_tag="ACHURS_003921"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFR1239537.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(6479..6907)
                     /gene="uspC"
                     /locus_tag="ACHURS_003922"
     CDS             complement(6479..6907)
                     /gene="uspC"
                     /locus_tag="ACHURS_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFR1239538.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
BASE COUNT         1769 a         1628 c         1819 g         1782 t
ORIGIN      
        1 atcaatgaat ttgaattcat gatgaagtga tgattttata aaacgttttt tcatttttgc
       61 gagatggctc tcattatacg tgttctgtta attaatgagc acagtgataa ttaatggcga
      121 aatgaatgac gtgcattttc cacatctttg agttgcggtt attacaccat ttcaaaaact
      181 caacgccagg taatgcggcc tattgactgg ttaaaaagaa gacatcccgc atgggtacca
      241 aagacaacaa ggatttccag gctaatctta tggattaatc tgctgtgcat tcgacaattt
      301 gtctgacaaa ttggctttcc cttatgtctt ttcccgctaa atttatgcac gttctcactg
      361 taattctgcg atgtgatatt gctctcctat ggagaattaa tttctcgcta aaactatgtc
      421 aacacggtca cttatctttt agataaaagg taatgctttg ttttccgatt aatttaacga
      481 aagtcattcg tttttgccct acacaaaacg acactaaagc tggagagaac catgcacaaa
      541 tttactaaag ccctggcagc cattggtctg gcagccgtta tgtcacaatc cgctatggcg
      601 gagaacctga agctcggttt tctggtgaag caaccggaag agccgtggtt ccagaccgaa
      661 tggaagtttg ccgataaagc cgggaaggat ttagggtttg aggttattaa gattgccgtg
      721 ccggatggcg aaaaaacatt gaacgcgatc gacagcctgg ctgccagtgg cgcaaaaggt
      781 ttcgttattt gtactccgga ccccaaactc ggctctgcca tcgtcgcgaa agcgcgtggc
      841 tacgatatga aagtcattgc cgtggatgac cagtttgtta acgccaaagg taagccaatg
      901 gataccgttc cgctggtgat gatggcggcg actaaaattg gcgaacgtca gggtcaggaa
      961 ctgtataaag agatgcagaa acgtggctgg gatgtcaaag aaagcgcggt gatggcgatt
     1021 accgccaacg aactggatac ggcacgtcgc cgtacaacgg gttctatgga tgcgctgaaa
     1081 gcggccggat tcccggaaaa acaaatttat caggtaccta ccaaatctaa cgacatcccg
     1141 ggggcatttg acgctgccaa ctcaatgctg gttcaacatc cggaagttaa acattggctg
     1201 atcgtcggta tgaacgacag caccgtgctg ggcggcgtac gcgcgacgga aggtcagggc
     1261 tttaaagctg ccgatatcat cggcattggc attaacggtg tggatgcgtt gagcgaactg
     1321 tctaaagcac aggcaaccgg cttctacggt tccctgctgc caagcccgga cgtacatggc
     1381 tataaatcca gcgaaatgct ttacaactgg gtagcaaaag acgttgaacc gccaaaattt
     1441 accgaagtta ccgacgtggt gctgatcacg cgtgacaact ttaaagaaga actggagaaa
     1501 aaaggtttag gcggtaagta atttgccgga aaaattcccc tctgcatgat gcagaggggg
     1561 tgtgaacgac cagtgattca cggagacgtt atgcaacagt ctaccccgta tctctcattt
     1621 cgcggcatcg gtaaaacgtt tcccggcgtt aaggcgctga cggatattag ttttgactgc
     1681 tatgccggtc aggttcatgc gttgatgggt gaaaatggcg caggaaaatc aactctctta
     1741 aaaatcctca gcggcaacta tgcgccaacc acgggttctg tagtgattaa tgggcaggaa
     1801 atgtcctttt ccgacacgac cgcagcactt aacgcgggtg tggcgattat ttaccaggaa
     1861 ctgcatctcg tgccggaaat gaccgtcgcg gaaaacatct atctcggcca gctgccgcat
     1921 aaaggcggca ttgtgaatcg ctcattgctg aattatgagg cgggtttaca acttaaacat
     1981 cttggtatgg atattgaccc ggacacgccg ctgaaatatc tctccattgg tcagtggcag
     2041 atggttgaaa tcgccaaggc gctggcgcgt aacgctaaaa ttatcgcctt tgatgagcca
     2101 accagctccc tttctgcccg cgaaatcgac aatcttttcc gcgttattcg tgaactgcga
     2161 aaagaggggc gggtgatctt atacgtttct caccgtatgg aagaaatatt tgccctcagc
     2221 gatgccatca ctgtctttaa agatggacgt tatgtcaaaa cctttaccga tatgcagcag
     2281 gttgaccacg acgcgctggt gcaggcgatg gtcgggcgcg acattggcga tatctacggc
     2341 tggcaacctc gtagttatgg cgaggagcgc ctgcgtcttg atgctgtgaa agcaccaggc
     2401 gtgcgtacgc caataagtct ggcggttcgc agtggtgaaa ttgttgggct gtttggtctg
     2461 gtaggggcgg ggcgtagcga attaatgaaa ggcttgtttg gcgggacgca aatcaccgcc
     2521 ggtcaggttt atatcgacca acagccgatc gatattcgta aaccgagcca cgccattgcc
     2581 gcaggcatga tgctctgccc ggaagatcgc aaagcggaag gcattattcc cgtgcactcc
     2641 gttcgcgaca atatcaacat cagtgccaga cgcaaacatg tgctcggcgg ttgtgtaatc
     2701 aacaacggtt gggaagaaaa caatgccgat cagcacattc gttcgctcaa catcaaaacg
     2761 ccgggcgctg agcaactgat catgaatctc tcaggcggaa atcagcaaaa agccattctg
     2821 ggccgctggt tatcggaaga gatgaaggtc attttgctgg atgaacctac gcgcagcatt
     2881 gatgttggtg ctaagcacga aatatataac gtaatttatg cgctggcggc gcagggtgtg
     2941 gcggtgctgt ttgcctccag cgacttacct gaagtcctcg gcgttgccga ccggattgtg
     3001 gtgatgcggg aaggtgaaat cgccggtgaa ttgttacacg agcaggcaga tgagcgtcag
     3061 gcactgagcc ttgcgatgcc taaagtcagc caggcagttg cctgagtaag gagagtatga
     3121 tgtcttctgt ttctacatcg ggttctggcg cacctaagtc gtcattcagc ttcgggcgta
     3181 tttgggatca gtacggcatg ctggtggtgt ttgcggtgct ctttatcgcc tgtgccattt
     3241 ttgtcccaaa ttttgccacc ttcattaata tgaaagggtt gggcctggca atttccatgt
     3301 cggggatggt ggcgtgtggc atgttgttct gccttgcttc cggtgacttt gacctttctg
     3361 tcgcctccgt aattgcctgt gcgggtgtca ccacggcggt ggttatcaac ctgactgaaa
     3421 gcctgtggat tggcgtggca gcggggttgc tgcttggcat tctctgtggc ctggtcaatg
     3481 gctttgttat cgccaaactg aaaataaatg ctctgatcac aatactggca acgatgcaga
     3541 ttgttcgagg tctggcgtac atcatttcag acggtaaagc ggtcggtatc gaagatgaaa
     3601 gcttctttgc ccttggttac gctaactggt tcggtctgcc tgcgccaatc tggctcaccg
     3661 tcgcgtgtct gattatcttt ggtttgttgc tgaataaaac cacctttggt cgtaacaccc
     3721 tggcgattgg cgggaacgaa gaggctgcgc gtctggcggg tgtaccggtt gttcgcacca
     3781 aaattattat ctttgttctc tctggcctgg tatctgcgat agccggaatt attctggctt
     3841 cacgtatgac tagtgggcag ccaatgacgt cgattggtta tgagcttatt gttatctccg
     3901 cctgcgtttt aggtggcgtt tctctgaaag gtggcatcgg aaaaatctca tatgtggtgg
     3961 cgggtatctt aattttaggc accgtggaaa acgccatgaa cctgcttaat atttctcctt
     4021 tcgcgcagta cgtggttcgc ggcttaatcc tgctggcagc ggtgatcttc gaccgttaca
     4081 agcaaaaagc gaaacgcact gtctgatgct tttttccaca acaatttaac gttttttccc
     4141 accacagcca gccgccacaa cggttggctg ttcttcattg caaatggcga cccccgtcac
     4201 actgtctata cttacatgtc tgtaaagcgc gttctgcgca aaacaataag aaaagagaag
     4261 gaggagagcc gggtgacaga accgttaacc gaaacccctg aactatccgc gaaatatgcc
     4321 tggttttttg atcttgatgg aacgctggcg gaaatcaaac cgcatcccga tcaggtcgtc
     4381 gtgcctgaca atattctgca aggactacag ctactggcaa ccgcaagtga tggtgcattg
     4441 gcattgatat cagggcgctc aatggtggag cttgacgcac tggcaaaacc ttatcgcttc
     4501 ccgttagcgg gcgtgcatgg ggcggagcgc cgtgacatca atggtaaaac acatatcgtt
     4561 catctgccgg atgcgattgc gcgtgatatt agcgtgcaac tgcatacagt catcgctcag
     4621 tatcccggcg cggagctgga ggcgaaaggg atggcttttg cgctgcatta tcgtcaggct
     4681 ccgcagcatg aagacgcatt aatgacatta gcgcaacgta ttactcagat ctggccacaa
     4741 atggcgttac agcagggaaa gtgtgttgtc gagatcaaac cgagaggtac cagtaaaggt
     4801 gaggcaattg cagcttttat gcaggaagct ccctttatcg ggcgaacgcc cgtatttctg
     4861 ggcgatgatt taaccgatga atctggcttc gcagtcgtta accgactggg cggaatgtca
     4921 gtaaaaattg gcacaggtgc aactcaggca tcatggcgac tggcgggtgt gccggatgtc
     4981 tggagctggc ttgaaatgat aaccaccgca ttacaacaaa aaagagaaaa taacaggagt
     5041 gatgactatg agtcgtttag tcgtagtatc taaccggatt gcaccaccag acgagcacgc
     5101 cgccagtgcc ggtggccttg ccgttggcat actgggggca ctgaaagccg caggcggact
     5161 gtggtttggc tggagtggtg aaacagggaa tgaggatcag ccgctaaaaa aggtgaaaaa
     5221 aggtaacatt acgtgggcct cttttaacct cagcgaacag gaccttgacg aatactacaa
     5281 ccaattctcc aatgccgttc tctggcccgc ttttcattat cggctcgatc tggtgcaatt
     5341 tcagcgtcct gcctgggacg gctatctacg cgtaaatgcg ttgctggcag ataaattact
     5401 gccgctgttg caagacgatg acattatctg gatccacgat tatcacctgt tgccatttgc
     5461 gcatgaatta cgcaaacggg gagtgaataa tcgcattggt ttctttctgc atattccttt
     5521 cccgacaccg gaaatcttca acgcgctgcc gacatatgac accttgcttg aacagctttg
     5581 tgattatgat ttgctgggtt tccagacaga aaacgatcgt ctggcgttcc tggattgtct
     5641 ttctaacctg acccgcgtca cgacacgtag cgcaaaaagc catacagcct ggggcaaagc
     5701 atttcgaaca gaagtctacc cgatcggcat tgaaccgaaa gaaatagcca aacaggctgc
     5761 cgggccactg ccgccaaaac tggcgcaact taaagcggaa ctgaaaaacg tacaaaatat
     5821 cttttctgtc gaacggctgg attattccaa aggtttgcca gagcgttttc tcgcctatga
     5881 agcgttgctg gaaaaatatc cgcagcatca tggtaaaatt cgttataccc agattgcacc
     5941 aacgtcgcgt ggtgatgtgc aagcctatca ggatattcgt catcagctcg aaaatgaagc
     6001 tggacgaatt aatggtaaat acgggcaatt aggctggacg ccgctttatt atttgaatca
     6061 gcattttgac cgtaaattac tgatgaaaat attccgctac tctgacgtgg gcttagtgac
     6121 gccactgcgt gacgggatga acctggtagc aaaagagtat gttgctgctc aggacccagc
     6181 caacccgggc gttcttgttc tttcgcaatt tgcgggagcg gcaaacgagt taacgtcggc
     6241 gttaattgtt aacccctacg atcgtgacga agttgcagct gcgctggatc gtgcattgac
     6301 tatgtcgctg gcggaacgta tttcccgtca tgcagaaatg ctggacgtta tcgtgaaaaa
     6361 cgatattaac cactggcagg agtgcttcat tagcgaccta aagcagatag ttccgcgaag
     6421 cgcggaaagc cagcagcgcg ataaagttgc tacctttcca aagcttgcgt aggagcggtt
     6481 aatctcccgt aagtggaact aacagcacat cgacctcact tgaggcaatc actcttttcg
     6541 ccgagcagga tgctcgcgaa aagaaactgt gattgtgatt accgcaaatc accaaatcga
     6601 aatgatgttt gtgacatact tccagaatat gttcgcttaa ctcaccgtag gcaataaacg
     6661 tcttgtcaac gggatacccc gcatcctgaa ttaacttatc aagaaagctt tgcgtttctt
     6721 cttgcatcac gctgcgtaaa tcttcaagca tcggcgcggc taattgattg tacatttccg
     6781 ggtcggaagc gagagtaatt aaactgatgt gtccctttac tggcctggcg atagataccg
     6841 cttttgccag cagttgctga ctttccggtg taaccgcaac agcgacaaga atattgctat
     6901 agctcataac ctgttcctta ttctgtgaac ttcaggtgac attaaagctt tactcctaat
     6961 ggagaaacga cgcaatccca actcggtcaa acaggtaa
//
LOCUS       DAZPDU010000207         6985 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZPDU010000207 DAZPDU010000000
VERSION     DAZPDU010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6985
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.209"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..499
                     /locus_tag="ACHURS_003923"
     CDS             <1..499
                     /locus_tag="ACHURS_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFR1239539.1"
                     /translation="WRRIPKFWNWIPASKIEKETRMYGTCETLCRELAAQYTENTPLM
                     LVVWSPEEIQALADGMEISLTGHEIRTVLARLEDIPEEQRIESGISSAAVMEIINNVS
                     ENRQVTVPAELLASLIQTAEQALWKREWAARDHGLAVPECVTRRQEVVNQARTLLKNN
                     THEND"
     gene            544..1314
                     /locus_tag="ACHURS_003924"
     CDS             544..1314
                     /locus_tag="ACHURS_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239540.1"
                     /translation="MNETLNALIYRHASNLLLAQGWPEETDVDQRNPKYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIHKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVRDAVCSVSCRVAEDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDYPCWLDEDDENLPVVLDAILNRGARFSSVEMYLVSECVEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVLEARSEIRSMADALAKIRG"
     gene            1727..2152
                     /locus_tag="ACHURS_003925"
     CDS             1727..2152
                     /locus_tag="ACHURS_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFR1239541.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGLDFLYAEASVYALAKKMMP
                     EYEGGFWHFIRLPDGGGYMMPDGDRFHLVNGENWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTHLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            2199..2621
                     /locus_tag="ACHURS_003926"
     CDS             2199..2621
                     /locus_tag="ACHURS_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFR1239542.1"
                     /translation="MYCTVKEIIREVLDTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFEHGADVSVVHDVVRELMEEKHASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            2618..2809
                     /locus_tag="ACHURS_003927"
     CDS             2618..2809
                     /locus_tag="ACHURS_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239543.1"
                     /translation="MNISTETREILRNYRAVINARRREMGQKPLTTAQIIDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            complement(3088..3336)
                     /locus_tag="ACHURS_003928"
     CDS             complement(3088..3336)
                     /locus_tag="ACHURS_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239544.1"
                     /translation="MSRGPLQRRRARARNGVAPYIPEAFCRDAGSAGHGATEAARPER
                     SLQAGGFHASDRSDSPKPRQRVRRRLAGAPAAACRSRA"
     gene            3852..4082
                     /locus_tag="ACHURS_003929"
     CDS             3852..4082
                     /locus_tag="ACHURS_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239545.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            4134..5495
                     /locus_tag="ACHURS_003930"
     CDS             4134..5495
                     /locus_tag="ACHURS_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HFR1239546.1"
                     /translation="MTLAAITMTAPEAASPVQMYRATYSPDDNKLRLYAASRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVEKLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDRYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGAMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVAETEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            5542..6105
                     /locus_tag="ACHURS_003931"
     CDS             5542..6105
                     /locus_tag="ACHURS_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFR1239547.1"
                     /translation="MHSQLRERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVT
                     LAEISNRDHILEPSAGTGAILRAIRDTAPEAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWQPVQYYSRIIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREVLPCGTFAYTRVPTMIIRLRA"
     gene            complement(6191..6646)
                     /locus_tag="ACHURS_003932"
                     /pseudo
     CDS             complement(6191..6646)
                     /locus_tag="ACHURS_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6909..>6985
                     /locus_tag="ACHURS_003933"
     CDS             6909..>6985
                     /locus_tag="ACHURS_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFR1239548.1"
                     /translation="MMGAENINRRRHIMAVRGINKVILVG"
BASE COUNT         1492 a         1942 c         2107 g         1444 t
ORIGIN      
        1 atggaggcgt ataccgaaat tctggaactg gattcctgct tcaaagattg agaaggagac
       61 gagaatgtac ggaacatgcg aaacgctctg ccgtgagctg gcagcgcagt atacggagaa
      121 cacgccgctg atgctggttg tctggtcccc ggaagagatt caggcactgg ctgacggtat
      181 ggaaatttct ctgaccggtc atgaaatcag gaccgtcctg gcacgcctgg aggacatccc
      241 ggaagaacag cggattgagt ccggtatttc ttccgctgcg gtgatggaga tcatcaataa
      301 cgtgagcgaa aaccgccagg tgaccgtccc tgctgaactg ctggcgtccc tgattcagac
      361 cgctgagcag gcattgtgga aacgtgaatg ggccgcccgg gatcatggcc tcgccgtccc
      421 ggaatgcgtc acccgccgtc aggaggtggt taatcaggcc cgcaccctgc tgaaaaacaa
      481 cacacacgaa aacgactgat gttatcgccg cctccgggcg gcgatacagg gagttcatga
      541 attatgaatg aaacgctgaa tgcactgatt tatcgccacg ccagcaacct gttgctggcg
      601 cagggctggc cggaagaaac ggatgttgac cagcgtaacc cgaagtatcc gggctggatc
      661 agcatttacg tgcggctgga tgcaccccgg ctggcgacgt tactcgttaa ccgccacgat
      721 ggcgtgctgc cgccgcacct ggcctccgcc attcacaaac tgaccggaac cggggcggaa
      781 ctggtactgt ccggcagtca gtggcagtcg ctgccggtac ttccggcaga cggaacgcag
      841 gtgtctttcc cgtatgccgg agaatggctg accgaagacg aaatcagggc cgttcttgat
      901 gcggtgcgcg atgcagtatg cagtgtcagt tgccgggtgg cagaagatgc gcggcgtatc
      961 cgggcggcgc tgaccacaac cggtcagacg ttgctgaccc gccagacgcg ccgcttccgt
     1021 ctggtggtga aggaaagcga ttacccctgc tggctcgatg aagacgacga aaacctgccc
     1081 gtggtgctcg atgccatcct gaaccgtggc gcacgttttt cgtcggtgga aatgtatctg
     1141 gtcagtgagt gtgtggagca tatcctgtcc agtgggctgg cctgcgatgt gctgcgtata
     1201 ccggatgaac cgccccgccg ctggtttgac cgtggtgttc tgcgggaggt ggtcctggaa
     1261 gcccggagtg aaatccgcag catggcggat gcactggcaa aaatcagggg ctgatgatgg
     1321 cggggaaaat ccccgccttt ttcctgccgg tccccggaaa gagcaaaagg atgtcgccgc
     1381 aggctccgct ttacccggcc atgcggggca tggccttgtg ggttttcagt tatgtggcct
     1441 cagcgtcgtg tgcgggctgt gccgtgcctc catcgtagcc gggcttctgc gggcgggttg
     1501 caaggtaaga tgaaccgctg cggtcacccg gtccctcctt cccgtctgcc gtgattttcc
     1561 ggtcgttcac ccgctcagat ttcgcggtct caccctccaa ctcccgccag ccgtgtgcgt
     1621 caggctgcgg cttcccttgc gcccttgcaa ccctgcaggc ccggcaaaga tggaggcacg
     1681 gcaccgcccg gtgtcgcgga atatgtgaac aacggaggaa aacatcatgc aatatgcgaa
     1741 acctgtcact ctgaatgttg aagagtgcga ccgtctctcc tttctgcctt acctgtttgg
     1801 cctggatttt ctgtatgccg aggcatctgt gtacgcgctg gcgaaaaaaa tgatgccgga
     1861 atatgaaggc ggattctggc acttcatccg cctgccggac ggtggcggtt acatgatgcc
     1921 ggatggcgac cgttttcatc tggtcaacgg tgaaaactgg tttgaccgta ccgtgagtgc
     1981 tgatgccgca ggcatcattt tgacttccct tgtgattaac cgccagttgt ggctgtacca
     2041 cgacagcggg gatgcaggac tgacccatct ttaccggatg cgcgatgcgc agttgtggcg
     2101 tcacatcgaa tttcaccccg aatgcaacgc gatttacgcg gcgctggact gattaacgga
     2161 cggggcggca atgccgcccc tgtaaaggag acgagaacat gtactgtact gttaaagaaa
     2221 ttatccgcga agtgctggat acagacgtgc cggacagtga atgcgttttt gccgtggtgc
     2281 tgacccgtgg ggatgtgcgt cacatagccc aggactggag tctgacagac gatgagctgg
     2341 aaaccgtcat gcagcggctg gacgatgcct ttgagcatgg tgcggatgtc agcgttgttc
     2401 acgacgttgt tcgtgaactg atggaggaaa agcacgccag ccgtcaggtg acagtcccgg
     2461 cggtgatgct ggaaaaagtg atggcgctgg caggcagtga aatgaagcgc ctgtatgccg
     2521 tcgggagtga gaacgggggc gacggtgatg cgttcgtcag ggaagaacgc gaagcaatgg
     2581 acgttgtgtt acaggcgctg gacggggagc acatgtcatg aatatcagca cagaaacccg
     2641 cgaaattctg cgtaattaca gggccgtgat taatgcgcgg cgtcgtgaaa tggggcagaa
     2701 accgctcacc accgcgcaga ttattgatga aatctgcgat tttgtggcga atcagcaggc
     2761 ggttttcctg ggcggtcact acatcttaca gggcagcaga aacaggtgat acaggggcag
     2821 gcggtggagg ccgcctgtca gcccgcgcga aaacccctgg caggctgacg cctgccggaa
     2881 gggaagcggg ccacgctggc gcgtcgcccg ttacccttcc cgtctgtgat gtgtctgact
     2941 caggagtgtt tctttcttat gttatggaac tgttcctgat gttgttgtcg ttgttccttt
     3001 atctgctgct ctgattctga ctactgcagt gttgttctgt ctgccggacg caccttcggc
     3061 tcccggctgc ctggcggcat ccggtcgtca ggcccgactc cgacaggcag ctgctggcgc
     3121 acccgccagt ctccgccgaa cccgctggcg cggtttcggg ctgtcgctac ggtccgatgc
     3181 gtgaaaaccc ccggcttgca gcgaccgttc cggtcgcgcc gcctctgtcg cgccgtggcc
     3241 tgcgctcccg gcatcccggc aaaaggcctc cgggatgtaa ggcgcaacgc cgtttcgcgc
     3301 acgcgctctt cgtcgttgca gcgggcccct gctcattgcc tgccgggtgc cacacacccg
     3361 tatgagtccc tgtcgccggg ggctgataca gccacaacct gccgcactga ctgtccaggg
     3421 taaatgaaca gcccacaaaa ttttttaccc aaaacccggt aaaaaatttt gcgttctgcc
     3481 caggacaggt gcgtcaggcc gtggcagtga tgccccttgc ggcgaaggtt ctcaaacggg
     3541 tgaagttcat accacaggga atacagcagc aggttcccgc gaaacgtcaa aaccgcggct
     3601 cccgaaactc aacgggtgat gttctttaaa aatcaggatt tatttcagac tccggcaata
     3661 cgcatttatg tgttctgtca gcatcctgct ggcaggtaat gagaacgcag gaacagaagg
     3721 aggaatacac cggagaacgt cagacgcaga tatgaaaaaa gcaggccggg gaggacctgc
     3781 ttaatccaca ccgcaaaaac tgttcgagca gtttctgcgg ttccattcaa cagtcataag
     3841 gagtttcatt tatgacggta agtattgttt caccttccgc agcggctgta aagccccgcc
     3901 gtcatccccg tttcagacgc gaagacatcc ccgcgccgga aattgacccc gtgctgaaag
     3961 ccttcgggcg acacatagcc cggagttttc accgggggcg tggtgtacac atccccgcca
     4021 tgaaaaacac ggccttcggg caggtgctcc gcacactcga actgaaacgc gcttttaact
     4081 gatgaggtga gccgccccgc gaggggcggc aacagacgga aggagaacat tttatgacat
     4141 tagcagcaat caccatgacc gccccggaag ccgccagccc tgtgcagatg taccgcgcga
     4201 cctactcacc ggatgacaac aaactgcgcc tgtatgccgc gtcacgcctc gacccggaga
     4261 cgtataaaaa agtgcatgat gccggttttc gctgggcacc caaacaggcg ctgtttgtcg
     4321 cgccagcctg gacaccgggc cgggaagacg tgctcctctc acttgccgga gagattgagg
     4381 atgaagacag cacgctcgct gaacgtcagg aagcacgggc ggagcggttt accggataca
     4441 gcggaaagcg ggccagtgaa tccgcacagg cacttgatga agtggaaaaa ctggccgcga
     4501 tgatcccgcc cggtcagccc attcttgtgg ggcatcacag cgaacgccgc gcccgtcgtg
     4561 atgcgcagcg tattgaaaac ggcatgaaac gtgccgtgat gctctttgaa cgtgcggaat
     4621 actgggaaga gcgggcgcgg tcggcactgc ttcacgcgaa gtataaagaa cgtccggacg
     4681 ttcgctggcg tcgtatcaaa aaaatcgaag ctgatttgcg caaggcggaa aagaccatcg
     4741 cacagtcgca gaaatatctg acgatgtggc gggctgaatc gctggatctg aatatggcaa
     4801 aactcatcag cagtcatgac catatcagcg cctgtttccc gctggacagg tatccgcgcc
     4861 cggcagaaaa aagccagtat gaaggaagtc gatcgttatg gtcggccctg gatgatgaca
     4921 tcatcaccac ggagcaggcc cgcgaaattg cgatccgctg tcatgaacgg cagattcagc
     4981 atcaacaacg ctgggttaac cactatcaga accgcctgat ctatgagcgt gccatgctgg
     5041 acgaaagcgg cggcgtggtt acccggacac aggattttga gccgggcgga caggttttca
     5101 gccggggcga gtggctgacc atcatccgcg tgaacaaaag caacggggcg gtgagttcag
     5161 tcacaacgcc gaattacagt tttctcgggt acagcggcgc gatgaaagtg acacccgatc
     5221 gcatcacgga ctacaaagca ccatcggcag aagaggctgc cgtcgccagt caggccgcga
     5281 agcgtccgcc ggtagtcaac tatccggggg aaggtttccg ggaaatgaca aaggcgcagt
     5341 gggccgccct gccccgggac tgtaaggccg tgcgcagtgt ggcagagaca gaagaccacg
     5401 gggcataccg ctaccgccgc acaatggaca ataatttccg cctggtgaat gtgtatatta
     5461 ccgacatgaa aatcacggaa atcccacaga aataaggtat atccccccgg gaaatccccg
     5521 ggggatgacg gagataatga catgcacagt cagttaagag aacgtatccg gctgatgcgc
     5581 gcaaggctgg ataacgccgc gccggttgct gaaatccggg ctgaatctca gctttttgtg
     5641 actcctgccc cggtctgtga tcgcctggtg acgctggcgg aaatcagcaa ccgtgatcac
     5701 attctggaac cctctgccgg caccggggcc atactgcggg cgattcggga taccgcgccg
     5761 gaggccatgt gtgatgcggt ggagattaac agcgggctgg tccggtatct gcgggaaaat
     5821 tttaacggtg tcagggtaca gtgtggcgac ttcatggaat ggcagccggt gcagtattac
     5881 agccggatca ttatgaatcc gccgttcagt cacgggcagg atatccggca tatcctgcgg
     5941 gccttttccc tgttgcgtcc ggggggcgtt ctggttgccg tctgtctgaa cgggccgcgc
     6001 cagcaggaga agctgttacc gttttctgat gtccgcgagg tgctgccgtg cggcacattt
     6061 gcttataccc gtgttccgac gatgattatt cgtctgcgtg cctgatgact ccgggcaggc
     6121 gggaggataa atgcccgtct gcccgcgccg ggaaaagacc gcgcgccggt ggcgctgaaa
     6181 ggggccgcgc ttacgcgccg cccctttcat tacgcggacg ttctgctgtg ggcttctgtc
     6241 ttttcagacc tgtcatttgc ctgttactga tgatgttgtt gttcctgcta ctgctactgc
     6301 tactgctact gctactgcta ctgctactgt tcttcttttt tctgctgctc tgattactgt
     6361 ctgttttgtt gttctcctgc ttatgccttt tccgggcgtc cccctggcgg ggcgggctgt
     6421 cgtgaacgga gccagcggag ctgtctccgc cctggcgggc ttccatccct gacgcaaaaa
     6481 cccgggtttg cagcgaccgg aacggtcgcg ccacccttgt cgcgccgtgg cctgcgctcc
     6541 cggcatcccg gcaaaagacg ccgggatgta aggagcaacg ccgtttcgca aaccgcgctt
     6601 cacgtcgttg cggcgggtcc ctgcccgttg ttctgtccgg tgccattcac ctgtttaagc
     6661 ccctgtcgcc gggggcatag acagccacaa cctgccgcac tgactgtcca gggtaaatga
     6721 acagcccaca aaatttttta cccaaaaccc ggtaaaaaat tttgcgttct gcccaggaca
     6781 ggtgcgtcag gccgtggcag tgatgcccca tgcggcgaag gttctcaaac aggtgatggt
     6841 gaataccgga gggaacaaaa ggcaggtccc cgcaaaacgt caaaaccgcg gctcccgaaa
     6901 ctcaacagat gatgggggct gaaaacatca acagaaggag acacatcatg gcagttcgtg
     6961 gcattaacaa ggtcattctt gtcgg
//
LOCUS       DAZPDU010000208         6962 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZPDU010000208 DAZPDU010000000
VERSION     DAZPDU010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6962
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.210"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(6..2789)
                     /locus_tag="ACHURS_003934"
     CDS             complement(6..2789)
                     /locus_tag="ACHURS_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFR1239549.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETLYQVSLPTTQKQNLQQVMAIFSEWSNAATFEILEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSLAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            complement(2846..5218)
                     /locus_tag="ACHURS_003935"
     CDS             complement(2846..5218)
                     /locus_tag="ACHURS_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFR1239550.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVTGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTCGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMAEHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFSLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            complement(5256..6872)
                     /locus_tag="ACHURS_003936"
     CDS             complement(5256..6872)
                     /locus_tag="ACHURS_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFR1239551.1"
                     /translation="MRKNNKTSVLLIIIILAMILGVVKIQVWLNDWNNDFFNALSQKE
                     TDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIRWREWLTDYYLNRWFADKNYYFTQ
                     IYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQSLSMLITFTVILWQSAGTLSFTV
                     GGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRPLNVEKQRSEATFRTNLVQHNKQT
                     ELIALSNAESLQRQELRDNFHTIKENWHRLMNRQRWLDYWQNIYSRSLSVLPYFLLLP
                     QFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDELAELAAVIDRLYEFHQLTEQRPT
                     NKPKNCQHAVQVANASIRTPDNKIILENLNFHVSPGKWLLLKGYSGAGKTTLLKTLSH
                     CWPWFKGDISSPADSWYVSQTPLIKSGLLKEIICKALPLPVDDKSLSEVLHQVGLGKL
                     AARIHDHDRWGDILSSGEKQRIALARLILRRPKWIFLDETTSHLEEQEAIRLLRLVRE
                     KLPTSGVIMVTHQPGVWNLADDICDISTVL"
BASE COUNT         1800 a         1589 c         1566 g         2007 t
ORIGIN      
        1 tgttattatt ttggcaataa tactccagta taagtattta ccggatgaga aagatattgt
       61 ttaacggcag tgttaacatt ctctaccgtc atttgtttca acaattgctc ctgctcagtc
      121 catgctgcag gatcgtcata ttgaataaga ctatttacaa tagtgttcgc taattgttga
      181 acgctacgct gttggatatc gaggctgcgc tgaacgtttt gctggtattc attcagttct
      241 tgctcactga tccctttagc cagacgctta accatcactt cattcgctaa cgttaacagt
      301 tcatcatgtc gttctggttg acaagtaaaa gccagcaaat gactgatatc tttggcctga
      361 ggatcaaccg agaggcgaga agaaacgctg tatgctccag atgcctgttc acgaatatta
      421 acgcgtagat cttttgccag tgcgacgtta aaagcatcga gcgccatacg cgtcgccaga
      481 ttaacaggtg tccgggaatc ataacgcttc cactgtgaaa cctgtgccac aggttcattt
      541 tgttctttta cagtaaccga tgcgttgtcc gtcgcgcgag ttaatggttt acctgcggct
      601 aatggcgaat cagagtgttt gattgatcct aagtaacgcg taattaacgc cacgagtttg
      661 tcttctgaga cattaccgac aatgacaaac gtgatatccg ctggagatga aaacaactgg
      721 cgatcggcag ccagcgcatc tgcggcagta aactgtacaa tctgattttc ttgcagtaat
      781 ttcgtgcggt catcagcata gcgcgtctca tacatctgct gggcgaattt ctccgctgga
      841 cgctggtcga gcgttttcaa cgcctgaatt tgagcatttt gtagcgatgc ccaaatattg
      901 tcgttaatcg tgctgtgggt gattcgctgg ttaatcaact gaaaaccagg ttcagggtta
      961 ttagtccgcg cgctaacgga gagcaacgta ttcatgccac tgactttact gctcatggtt
     1021 accgaatttt ccgcactcca gcgtttcagg ctggaagagg agagctcacc aacgccgctt
     1081 ccgcttactg ctttatttgc cagcgcgata agtgattttt gctgcgcagg gaaacttaaa
     1141 tcgcctttat tagatacggc agtaatttgt agcttttgct cttcacccgc ggattttgcc
     1201 agaataaccc tggcaccatt ggaaagtgtt aatgatgtca gattctcagc cagcgtttct
     1261 ttgctgctaa tttccgcctg tgggtcagcg tctactgtta acgataaatt tctgcctggg
     1321 aagatgtaag ccgccagctt tttgttggcg tactcttttt ccagcgccag gatagccgca
     1381 agagacaatg cttttttggc ggcaagctca ttgtttacca tttgctccca aaatgcgtcc
     1441 tggttctttc ttaactgctg ccatttttcc gccagacttt gcacggtaat ttgctgccac
     1501 agacgtttcg aaagttgata tgtctcttcc ggcgacaaga acggcgtatt atttaatgag
     1561 ctggatgcca ggcgactggt cagcatacgt aaatcacgtt cagcttgctg atcaaccgca
     1621 tttttcagcc aggtgaggcg ggtagatttg acatcatcga gttcttcagc agaaaaacca
     1681 tgctgatcaa tggttgccaa ctctgccatt aatgcatttg cagcatcctg catattatcg
     1741 tctcgtgcat ttacacggaa aaacagcgac tgataatcgg gtgcaatttt aacgctgcgc
     1801 gcagtgccgc cagaaatagt cttcaactcg cccgactgta tgcgttcctg cagacgttga
     1861 ttgaacagct gaactaacat gctccattca gcttgttcga caaagctttg ctcatcgttc
     1921 acttgtacca ttggcaggcg ataatagagt gcgatgccgt tcactcggtt ttctttatca
     1981 ttaatgatat taaagcgcag gtggttttcg gcttttgtcg gccagacgcg attttcagct
     2041 gctttgttag ccggaagctt acttaaatta tcctttatca gcgccagcgc ttctttactg
     2101 tcgatatcgc cgaccacgat aaaggtcata ttatttggtt gataccagcg ttgataaaat
     2161 tggcgcaatt gtgccggtgt gaccgtggcg acagtatcca tcaggccgat aggttcacgg
     2221 tctaaattac gggtatttgc cagcaggaaa gggcggcgcg cctgagaggt gcgccatttc
     2281 gcatcctgat gggcacgcca ttcctcagta attacgccgc gttcagcgtc tacttcgagt
     2341 atttcaaagg ttgcggcatt actccattca ctgaagattg ccatcacttg ttgcagattt
     2401 tgtttctgcg tagtcggcag actcacctga tacagcgttt cgtcatagct ggtataggca
     2461 ttaacatcgc gaccgaaacg caggcccatt gactcaaatg tttcgatgac tttattaccc
     2521 ggccatgttt ttgtgccgtt aaacatcata tgttctacaa aatgagccac gccgcgctca
     2581 ttgtcttctt cctgcaatga accggtatga atttgcagcc ataaatttac ctgatccttt
     2641 ggttgagcat gcggataaat catatatcgc aagccattgt ccagttgccc ggtaattaac
     2701 ttttcatcct gcggtaaggc ggcggcaatc agccgcccgg ggagcaacag agttgccact
     2761 aacgtcagta agaaacagag gtttctcata attatctcca tgcgaaaacc gggcgaattt
     2821 acccggttaa gtaaaatccg aactattaaa atttcatgct gacatcaagc cagaaagtac
     2881 ggccactggc gtaggtcgcc acaccggtgt tcgttgtatc aacagcggtt ttgctatcca
     2941 gtacattgag aatatcggca ctgatcgtca ggttttgttg tttcagaaat tgtggcgtcc
     3001 aggacaagcg ggtgtcccag gtcaggctgc tatccagttt ttcgtcaaca tactgcttgt
     3061 aatctgaata ttcgctgatg tattgcgcat ttgtcttacc aaggataatg cgagctttac
     3121 gcgcttcttg ccaggccagc gtattcgccc acactaaccc gctcggttga tgcgtgaaat
     3181 ccatgtttaa ggagatcttt aatgggttat taaaatctgc cactggaaca ctatcgtaag
     3241 agaccagatt accgttataa accacctggt tatcaccggt attgctctcc tcataaccat
     3301 tgtttaacga caagttgccc ttgctcttga tatagctaaa gacaatttgt gggttaatat
     3361 ctacttggcg gatatgcagg ggttcggcca gttcaaaact gaggctgaac gaatgcgttt
     3421 tggttttgcc atcgttgtta tattcagtaa tggtggtttt agtcgcgctg tcggtacgac
     3481 tgcttttgct gatttgatca tgcgcttcac ggtaaacata gtttgcacgc gcaataacgt
     3541 tcttaccgat tttctgctgc aatcccattg ccagttcatc gttataaggc gttttcaaat
     3601 cctgataacg cgtcagagtt ttattacctg ataccgattc cgtccagcta ttgcggatat
     3661 cacgtaatcc catatcaaga atattcccgc catagtaacg gttataaccg gcggtaatca
     3721 ttgaggtttg atcagcaaaa atatcccatt ccgtcataaa gcgcggggag atattgtggt
     3781 ttgacagata gttgtcatag tcataacgca caccgggcat taacgacaca ttccgccagc
     3841 taatgtgctc cgccatatac agcgtgtagt tgtcaattcc caggctgcct ttacctttat
     3901 ggtaaatggt atggttagtt ttctttcctg cagcattaat cacataggat tcagactggt
     3961 tatggcgttc agtccatgca tcggaataga tgtattccgc gccgaagtag ggttgatgcg
     4021 aaacatcacc cacggcgaat ttttgccagt ccaggcgtgt tttgaaggtg taattatcta
     4081 cagcctggga aatgtgtcct aatccgccac aggtacaacg ccctgtaata tcaccatatg
     4141 tacatgaaag ttcggtgtac cagatgtcat gatcgtgacg ggtataatca ctaatatgat
     4201 cccaaccaac ggtggtacgt agtttggccc atgcgagttg cgtatccata tcccatgcca
     4261 gaccatagga tttattaccc atttcgcgat cagactgcgg gaaggtgctg gtattataat
     4321 cacggctgga gccggtatat tttaaggtta aatcgtgggt aaagcggtcg ctggcaaacc
     4381 aggtaaattt gctcaatgca gtatcgataa cgtttttata ctgtgcccga ccggtgacaa
     4441 tgccgtcatt cgaaacataa tccgcgcggg tgatatcaga ctggcggcgc gataaaccgg
     4501 cggtaacgcc gaagttatca gcgagttcct gattaaacga caaggtataa aagttctttt
     4561 taaaatctgg ggagtaataa gtacttcctg aagagccttg attaaatgcg ctcttgttat
     4621 tctcatcgat atgcgatgtt aaccagtccg aacgcgtagt gcgataaccc aatttcacct
     4681 tgctatcatc agcgttgaag cgtttgatct ttgcatcaat taccccgcca ttgaagcgac
     4741 caaattcaac cggcacaaaa ctgtcataaa gcgtcacatt gtccagtaag ctgacatcaa
     4801 gataataccc ctgtgacatc ccgctaatat tggttgcact actggcatcg gactcattcg
     4861 ctgggttcag gttattagtt gcactaatac cgtcaatcaa ataggcattc tggtagggcg
     4921 acgcaccgtg aatagagatt ttctcagggc gaatatctcc ctggttcaac gaggtacttt
     4981 gcgttgaatc catgcgtacc gcagggttgg ttctcagcag atcgctgata ttgccattcc
     5041 cggtcggtaa acgctcgatg ctttcactgg tgtaatgcgt gttgccgttt acgggtacag
     5101 ggacaggagc atagaccgtc atctcttcaa aaaaatgcat gtacatgttt ttgtcatcga
     5161 cggcctgcgc cacatcagcg ccacataaaa tgacgccagg aataagaact cgcttcattt
     5221 atttatatct ccgtacagca gggcttttat tgttgttata aaaccgtgct aatgtcacaa
     5281 atatcatcag ccaggttcca gacaccgggt tgatgtgtaa ccataatgac gccgcttgtg
     5341 ggtagttttt cacgcactaa acgcagtaag cgtatagcct cttgttcctc aagatgagag
     5401 gtagtttcgt caagaaatat ccatttcgga cgtcgtaaaa ttaatcgtgc cagggcgata
     5461 cgttgttttt cgccgctgga aagaatatct ccccagcgat cgtggtcatg aatacgcgca
     5521 gccaatttcc ccagaccaac ctgatgcagt acttcgctca acgatttatc gtctacgggc
     5581 aggggaagtg ctttacaaat aatctctttc agtaagccgc ttttgattaa cggtgtttgt
     5641 gacacatacc aactgtcagc aggagaagaa atatcacctt taaaccacgg ccagcagtgg
     5701 gataatgttt taagcagtgt ggtttttccc gcaccagagt agcctttcag taatagccat
     5761 ttgcctggcg aaacatgaaa gttcaagttc tctaatatga tcttattatc aggcgtacga
     5821 atactcgcat tagccacttg taccgcatgt tggcaatttt taggcttatt cgtagggcgc
     5881 tgttcagtga gttgatggaa ctcatacaag cgatcgataa ccgcagccag ttcagcaagt
     5941 tcgtcatatt tataaataaa ccagcttaaa ttgttcgata ctagcataaa tgcctggcgc
     6001 gatttcatca gtccgcccag attgatctgc ccgctaataa actgcggcaa taacagaaag
     6061 tagggaagaa cgctaagcga gcgcgaataa atattttgcc agtaatcaag ccaccgctga
     6121 cgattcatta aacgatgcca gttctcttta atggtatgaa aattgtcgcg caactcctgg
     6181 cgttgtagag actcagcatt gcttagcgca atcagttctg tctgcttatt atgctgcaca
     6241 agattagttc gaaatgtcgc ttcgctacgt tgtttttcca cattaagcgg acgaatacgt
     6301 ttacccacct tatgggtaaa taaagttcca ccgatcacaa tgagcacgac ggtatagacc
     6361 atatacccct ggatattcca ttctgttcca ccaacagtga atgagagcgt acccgcgctt
     6421 tgccaaagaa taacggtaaa ggtgatcagc atgctaagtg actggatgaa gccaaatgaa
     6481 agactgagcg ttttgctgat cagtaagaga atgtcttcag cgatacgttg gtcagggtta
     6541 tctgtatttt tatgctcgcc gtagatctgc gtgaagtagt agtttttatc tgcaaaccag
     6601 cgattaaggt agtaatctgt taaccattcg cgccagcgaa tagtcagtag tttaattaac
     6661 catgttttgt ttacggatat taatacaaag atccccagca gggcagggaa ccaaagaaca
     6721 agctgccaaa gcttatcggt ttctttctgg cttagcgcat tgaaaaaatc attattccag
     6781 tcgtttaacc agacctgaat tttaaccacg ccaaggatca ttgcgaggat aataatgatt
     6841 aacaacactg aagttttatt attcttacgc agccaaaagg gcttcagcag acagagatac
     6901 tttgctatca acatataaaa cgtaatggga atggttatca ttagcgaaaa ttgattgtgc
     6961 ca
//
LOCUS       DAZPDU010000209         6833 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZPDU010000209 DAZPDU010000000
VERSION     DAZPDU010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6833
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.211"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(282..767)
                     /gene="sixA"
                     /locus_tag="ACHURS_003937"
     CDS             complement(282..767)
                     /gene="sixA"
                     /locus_tag="ACHURS_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFR1239552.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(970..3114)
                     /gene="fadJ"
                     /locus_tag="ACHURS_003938"
     CDS             complement(970..3114)
                     /gene="fadJ"
                     /locus_tag="ACHURS_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFR1239553.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3114..4424)
                     /gene="fadI"
                     /locus_tag="ACHURS_003939"
     CDS             complement(3114..4424)
                     /gene="fadI"
                     /locus_tag="ACHURS_003939"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFR1239554.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4604..4888)
                     /locus_tag="ACHURS_003940"
     CDS             complement(4604..4888)
                     /locus_tag="ACHURS_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFR1239555.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5260..6600
                     /gene="fadL"
                     /locus_tag="ACHURS_003941"
     CDS             5260..6600
                     /gene="fadL"
                     /locus_tag="ACHURS_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFR1239556.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYISWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1702 a         1828 c         1624 g         1679 t
ORIGIN      
        1 aatcaagttt caattacatc ctgcctgatt attcaactct attttctcta ttatctgtat
       61 taataaatga caatgagtgt caaacagaca ttattcattt caacatgtaa ctttgggcaa
      121 aatcaagata cgcgcagaag aataatttcc gaatgaaagt agaaagtgtt ttatttatag
      181 atggggagag tcttcgatag tagtgagatt gacagatatc cgctgcctga tgagcaatta
      241 tgcccaatga atctacctca ttgggcataa tttggaactc atcagatagc ttttgccatc
      301 ttcagattac acgggctcat ctgccagttg aatgtgccgt ttccgctttc atcaagcgtt
      361 acgctggcaa tggccgaggt ggtaaacata ggcggcgttt cgcccggaca taactcggca
      421 accagatacc cgactaacgg taagtgagag atcaccagta ccgaagcaac accttcattg
      481 gtcaacgcct gcaaataggc actgaccaga ccaacatcgc cgcagggcgt taactccggc
      541 agaacttctg cactggaagg caggttcaga caatccccta cttcttccag tgtttgctcg
      601 gctcgcagga acgggctcac cagaacgcgt tcgatttcca ctttttgacc tttcagccag
      661 ttcgccatca ggcgagattc gtcacaacca ttagtggtca gaggacgaac cgaatcactg
      721 gcggcatcaa gggctgcgtc gccgtgacgc atgataaaaa cttgcatatt gcaccgcttt
      781 tgttaaccag attcgtccgt tttctttcat ctgaaaccag agaaagaaaa cggtggccgg
      841 cattgtgcct tatccattca ccgaatgaaa cgctgttttt tacctcaatg gcgtaagtat
      901 agtcaatatg cgtttacatt ttgaccaaca ctccgccatt cagcgcggat tcatatagct
      961 ttgaccttct tattgaaggt cagttgcagt tgttttccaa aaactttccc cacgcgcgcc
     1021 catctcgacc aaacgctcgc aaggggtaaa acgggaacca tactgcgtgg ccagtcgttg
     1081 cattattgca accacttcgc ccgcgccgag agaatcgata tagcggaacg gtccaccgag
     1141 aaatggcgga aaaccaatgc caaataccgc gccaatatcc ccgtcacgca cgctacggat
     1201 aacctgctca tcaacacaac gtactgcttc attcagcatc aacatcacac accgttcagc
     1261 aacctgcggt gcggagagtc gcccctgccc ttgtgcgcca atgagcgggt aaatggcggg
     1321 atcgacctgt tttttgcttt tacgcccttt ctgaccataa agatagaaac cccgaccatt
     1381 ttttctgcct ttgcgatcgt cgttcaaaat tgaagaaaca acatttgcag gcgcgctaaa
     1441 acgttctcca taagcggctt ccagtacagg aataatttta gtcccggtgt cgattcctac
     1501 ctcatccaaa agttggattg ggcctaccgg aaaaccaaat ttcactagcg cggcatcaat
     1561 gtgctctacc cgttcacctt cggtcagcat gcggatagct tcattaatgt aaggcgctaa
     1621 gatgcgattg acgtaaaacc cggctttgtc acgcacgaca atgggcgttt tgccctgttt
     1681 tttcgccagt tttaccgtag tggcgatggt ttgcgccgat gtacccgcat gaggaataat
     1741 ctctaccagc ggcatttttt ccacaggact gaagaaatgc aggccaataa cttgctcagg
     1801 tcgcgcggcg tgagcggcga tatcaccaat cggtaaagat gacgtgttcg aagcaaaaat
     1861 ggtatgagcg gcgcaatttt gctccacttc cgccaccatc tgttgtttta attcgagatt
     1921 ttcaaacacc gcttcaataa tcagatcgcg atgggcaaag ccgcgatagt ccgtcgttcc
     1981 ggagattaat gccagctgtt tgtcacgttc gctggctttg agatgacgac ggcgaacttt
     2041 gccctccagc tgatcccaac tgtacttcag cgcatgattt atgccctgcg ggttgatatc
     2101 tttaattctg accggaagcc ccgctttaca agcagtgaca taagcaatac cgccgcccat
     2161 caagccacca cctaaaattc ccacgctgtt taatggcgca ggcggtgcat cactaccggg
     2221 atctttcttc acgtccgtac tggcaaaaaa gatactacgc aaagcttgcg attgcggcgt
     2281 catcgccagt tcgccaaagg cgcgagcttc agcgtcatag ccgctgctgg tgccctgcgc
     2341 taatcccgtt tcaacaacct ccaggatgcg ttctgtcgcc ggataattgc cttgagtttt
     2401 gtgttctgtt ttcttgccga ccattttgaa cagcagcgca cgacctaacg gccccgccag
     2461 aatacgctcg cgtacaggta gagggcggga agatgggcga tccttcttcg ccagctcaac
     2521 agcggcttcc agcaaaatgg agtgcggaac aacgtcatcc accagcccca gctttaatgc
     2581 ctgtttcgcc cgaagttgtt ttccggtgag aatcatctct aatgctgtac tgacgcctat
     2641 cagacgcggt aaacgctggg tgccgcctga accgggtaac aatccaagtt gtacttcagg
     2701 caaaccgagc accgttttag gatcgtcagt acaaacgcga ccgtggcacg ccagcgccag
     2761 ctccagccca ccgcccaggc aagcgccatg aatagccgcg ataaccggaa tgggcaaagc
     2821 atgaatctcc gccatcaact gttggccctg ccgcgccagt gcttccgctt cttgcgccgt
     2881 tttgcagttg ccaatcatgt tgatgtctgc tccggcgata aaattgtccg gtttcgcgga
     2941 gataaacacc acgccgcgca actctttgtt ttcacggagt tgcttaataa tggcgcgcac
     3001 ctgcgaggca aattccgcct tcagggtatt cattttctca tccggtacat cgatggtgat
     3061 gatagcaatg ttgtccagac gaacattaag agtaaacgct gatgccattt ccattattcc
     3121 gcctccagaa ccattgccgc gccaagccca ccggcagcac aggcggtaac taaaccaaat
     3181 ccaccgccgc gacggcgaag ttcatgcaat gtctgggtaa tcatccgcgc gccggtcgcc
     3241 gcgaagggat gcccgtaagc aatcgaaccg ccaagcacgt taaatttgct atcgtccact
     3301 tcgccagtgg catgtgcacg ccccagtact tcacgagcaa aacgttcact acccagcaac
     3361 tgaatattcg ccagtgtctg agctgcaaag gcttcgtgca tatcgatcaa tgtcagttcg
     3421 ctcatcgtca aaccggcacg ttccagcgcc agcggtgttg accaggctgg accgagcaac
     3481 atgtcctgcc agacatcaat cgcagtaaat gcgtagctgc gcagataccc cagcggcacc
     3541 agccctaatt ctttcgcccg ggattcagtc atcaggatca ccgctgccgc gccatcggtc
     3601 agcggcgtac tgtttgccgc cgttaccgtt ccgtgtttgc gatcaaacgc cgggcgcagc
     3661 tttgcgtaat cggcaagcga ggaattaccg cgaatattgt tgtcttcgac aagcggttgt
     3721 ttataaggag ggataaaggc agtcatcacc tcttctttga gttttccgtc tgaccatgcc
     3781 tgagcggcac gctgatgcga acggtgcgct aatgcatctt gctgttctcg ggtgatgccg
     3841 taggttttcg ccatttgttc ggcggtgtcg cccatccgca agccagtaga atattctgct
     3901 accgcaggcg gtacgggcat taagtcgcgc aaacgcaggc gagagaagag tttcaggcgc
     3961 tggctcatgg ttcgagcttt gttgacatca accagcacgc gcgccagttt tttactgacg
     4021 ccaattggca atacggaaga ggaatctgcc ccaccggcaa tccccgctcg gatagttccc
     4081 gccatcaggc tttctgcgac gtttgcaacc gcctggaaac tggtagcgca agcgcggctg
     4141 acgctgtaag catcggtatg cacattcatt ccggtaccga gaacaatttc acgcgcaatg
     4201 ttgggggctt caggcatttg tacgacctga ccaaagacca gctgttcaat cacttcagcg
     4261 gggatctcgc tgcgtgccag cagttcgcct accaccatct tccctaaatc aaccgcggga
     4321 atgccatgaa aagccgtcgc ctggcgggca aaaggcgtac gtaaaccgct aacaatggcg
     4381 atacgatcgc cctggcgggt aaccagcggt aaaacctgac ccataacact cccctgtaaa
     4441 aaaataaata aagtggtctg acctgatcat agtcttaact atttttttac atttagccaa
     4501 gcggagaaaa aggaaagtgg gagctatgac acagtgaaga caggagaaag aaaagaaaac
     4561 gcccctgcca gcaactgaca ggggcgaacg tacccgagag gaattaacgc agacccagct
     4621 ggaagatcag catttcagct tcgcaggcaa aagtgaaatc gatatccagg cgcacaccgt
     4681 cagactcttc agtgaaagtc ggggagattt tgcacggttc ggattccacg ctacgggctt
     4741 tttcagtcag cgccgccagc gtttgttctg cttctgcgcg gtttgcaaac acgcggctgt
     4801 aagacgcggt gcagtcggag ttgtccataa tggtgccaac atccatacag cagcaaaccg
     4861 gggtttcatc agcactacat ttactcatcg ttgatttcct ctgtatgtgc acccaaggtg
     4921 ccagataaac gttgtggata ttttacgctt ccggaaagtg ctgctccagt tgttaattct
     4981 gcaaaatcgg ataagtgacc gaaatcacac ttaaaaatga tctaaaacaa aattcacccg
     5041 aatccgcgag tgcgcaacct ccaaattttg ccagctggat cgcgtttctt agatcatatt
     5101 tgaaaaaaga tggaaacata cttgcagcat tccagctggt ccgacctata ctctcgccac
     5161 tggtctgatt tctaagatgt acctcagacc ctacacttcg cgctcctgtt acagcacgta
     5221 acatagtttg tataaaaata aatcattgag gttatggtca tgagccagaa aaccctgttt
     5281 acaaagtctg ctctcgcagt cgcagtggca cttatctcca cccaggcctg gtcggcaggc
     5341 tttcagttaa acgaattttc ttcctctggc ctgggccggg cttattcagg ggaaggcgca
     5401 attgccgatg atgcaggtaa cgttagccgt aaccccgcat tgattaccat gtttgaccgc
     5461 ccgacatttt ctgcgggtgc ggtttatatt gacccggatg tgaatatcag cggaacgtct
     5521 ccgtctggtc gtagcctgaa agccgataac atcgcgccta cggcatgggt tccgaacatg
     5581 cactttgttg caccgattaa cgaccaattt ggttggggcg cttctattac ctctaactat
     5641 ggtctggcaa cagagtttaa tgatacttat gcaggcggct ctgtcggggg tacaactgac
     5701 cttgaaacca tgaacctgaa cttaagcggt gcgtatcgct taaataatgc atggagcttt
     5761 ggtcttggtt tcaacgccgt ctacgctcgc gcgaaaattg aacgtttcgc aggcgatctg
     5821 gggcagttgg ttgctggtca gattatgcaa tctcctgccg ggcaaactcc tcaagggcaa
     5881 gcattggcag ctaccgccaa cggtatcgac agtaatacca aaatcgctca tctgaacggt
     5941 aaccagtggg gctttggctg gaacgccgga atcctgtatg aactggataa aaataaccgc
     6001 tatgcactga cctaccgttc tgaagtgaaa attgacttca aaggtaacta cagcagcgat
     6061 cttaatcgtg cgtttaataa ctacggtttg ccaattccta ccgcgacagg tggcgcaacg
     6121 caatcgggtt atctgacgct gaacctgcct gaaatgtggg aagtgtcagg ttataaccgt
     6181 gttgatccac agtgggcgat tcactatagc ctggcttaca tcagctggag tcagttccag
     6241 cagctgaaag cgacctcaac cagtggcgac acgctgttcc agaaacatga aggctttaaa
     6301 gatgcttacc gcatcgcgtt gggtaccact tattactacg atgataactg gaccttccgt
     6361 accggtatcg cctttgatga cagcccagtt ccggctcaga atcgttctat ctccattccg
     6421 gaccaggacc gtttctggct gagtgcaggt acgacttacg catttaataa agatgcttca
     6481 gtcgacgttg gtgtttctta tatgcacggt cagagcgtga aaattaacga aggcccatac
     6541 cagttcgagt ctgaaggtaa agcctggctg ttcggtacta actttaacta cgcgttctga
     6601 taacgcgttc gcctggataa agtcacctgc atagcaggtg actttaactc ccccacttca
     6661 ccgaaagtag tgtcctcatt gcttacctcc tgagttttgc aaacagcctg ttggcagctt
     6721 gccctttcaa atcaataagc ggtatccaca gacaaacctg aagggaaagg catattttca
     6781 ggcgttctgc tcgtccttct caaagagttt acttttctgc atttccagga tac
//
LOCUS       DAZPDU010000210         6832 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZPDU010000210 DAZPDU010000000
VERSION     DAZPDU010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6832
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.212"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..214)
                     /gene="cas6e"
                     /locus_tag="ACHURS_003942"
     CDS             complement(<1..214)
                     /gene="cas6e"
                     /locus_tag="ACHURS_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFR1239557.1"
                     /translation="MAVWGTHRQSGAVAQQGGTMYLSRITLHTGQLSPAQLLHLVDRG
                     EYVMHQWLWDLFPGGKERQFLYRREEL"
     gene            complement(139..885)
                     /gene="cas5e"
                     /locus_tag="ACHURS_003943"
     CDS             complement(139..885)
                     /gene="cas5e"
                     /locus_tag="ACHURS_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFR1239558.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            complement(896..1951)
                     /gene="cas7e"
                     /locus_tag="ACHURS_003944"
     CDS             complement(896..1951)
                     /gene="cas7e"
                     /locus_tag="ACHURS_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFR1239559.1"
                     /translation="MTTFIQLHVLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEDKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRIAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(1963..2499)
                     /gene="casB"
                     /locus_tag="ACHURS_003945"
                     /gene_synonym="cse2"
     CDS             complement(1963..2499)
                     /gene="casB"
                     /locus_tag="ACHURS_003945"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFR1239560.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAAVSANVKAIDERQPFAAQLAAVMSKGRFTRLS
                     AVKTPDELLRQLRRAVRLLNGSVNLDSLADGIFRWCQESDDLLNHHRRQQRPTEFIRI
                     RWALEYYQAGDADNEQNQ"
     gene            complement(2496..4058)
                     /gene="casA"
                     /locus_tag="ACHURS_003946"
                     /gene_synonym="cse1"
     CDS             complement(2496..4058)
                     /gene="casA"
                     /locus_tag="ACHURS_003946"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HFR1239561.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDISAPRADL
                     QGAVWQFLLGLLQTSFAPKDHRRWDDIWEDGLEAEKLREALLSLEHAFQFGPDSPSFI
                     QDFEALTGDKVPVASLLPEIPGAQTTKFNKDHFIKRGVTEYLCPHCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQTPLWRKLWINVMPQDEADLPLPKKFDD
                     LVFPWLGPTRISELAGAVVTDEQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQSDA
                     LLSLMTTKNYGANYAMWQHPLTPYRVLLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLNKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDADELLSKWNKEIWLFARQDFDERVFTNPYEPVDLERIMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            complement(4156..6831)
                     /gene="cas3"
                     /locus_tag="ACHURS_003947"
     CDS             complement(4156..6831)
                     /gene="cas3"
                     /locus_tag="ACHURS_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HFR1239562.1"
                     /translation="MKDKNIVTFFDFWGKTRRGEKDGGDDYHLLCWHSLDVAAMGYLM
                     VKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPPELKIQEGA
                     RKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTTGHHGRPPD
                     RMDELDNFLPEDKAAARDFLLEIKPLFPLIEIPAFWDDDEGIELIKHLSWYISATVVL
                     ADWTGSSTRFFPRVAHPMDIKGYWQKTLVQAQNALTVFPLKAKVAPFNGINTLFPFIE
                     NPTPLQQKVLDLDISQQGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQGLFFGLPT
                     MATANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLVGSEEPDEQ
                     TFSQGCAAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNKILLADEIH
                     ACDAYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGLQEAPFLEK
                     DDYPWLTHVTKSDVNSHRVATRKDVERSVSVGWLHSEQECIARIESAVSQGKCIAWIR
                     NSVDDAIKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKEDSSQRAGK
                     VLICTQVLEQSVDCDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRDGKDERSPP
                     ELLILAPVWDDSPGDEWFGSAMRNSAFVYPDHGRIWLTQRVLREQGAIQMPHAARLLI
                     ESVYGEDVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFRPGYAANINDYLPEKLSTR
                     LAEESVSLWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLLEGEAFRLW
                     CIEQRQDPEMANVILVNDDESCGYSATEGLIGKVG"
BASE COUNT         1650 a         1860 c         1621 g         1701 t
ORIGIN      
        1 ggagttcttc ccgacgataa agaaattgcc tttctttgcc gccagggaaa agatcccaca
       61 gccactgatg catcacatat tcaccgcgat ccaccagatg cagcaactgc gcaggcgaaa
      121 gctggccggt atgtaaggtt attcttgaga ggtacatggt tcctccttgc tgagccacgg
      181 cccctgattg acggtgcgtt ccccaaacag ccactgctga cgatttaaag gaacatcccg
      241 gcggcgtaat attttgctcg cgaccaggcc gtcgtgtttc ccttcccacc agcattcatc
      301 ctgaagtttc gggagtgaga ctttcagttt atggaaatga tcctgatact gttggtatgc
      361 attacataag gcatcacacg cgttgccttc gagcagtaac ggcgcaagag gtaatgccag
      421 cggatgactt tttcgcccca gataaagcgg aaaaactgga tggcgtaaac cgtcctgcaa
      481 ctgttcgagg ctgtaaggcg catcggctgt tgttgccacc gccaccatcc accaggcatc
      541 ggtgtagtag tcgcgccggg agataatcgc gctcaaaagc tcagggtcgc tcaactcttc
      601 acgacggctg aaataacgtg ctttacgcac ctcttttggc atctggaccg tgtgataatc
      661 ccgtgcccag cgcgggttac ggctggcgca aaccaccagt gaatagtggc gattaaacgc
      721 gtttaagcgt tcggtatcat cacgccgaat ccctaccccg gcagccagta gccccagcaa
      781 tgctgagcgc gaaggaagtt catgggtatg acgaacttca cctggagcat cgacacccca
      841 ggacgccatc ggcccatgaa gctgaaaaat cagatattgg ctcataagcc gccccttacg
      901 cgcagataaa gtccagcacg tccttcatgc ttccctgttt gttcatcacg tcaaaacttg
      961 cgcattcggt cttctgttcg tagaccgtat tcatgttttc gcgaagcgtt gtaatacgct
     1021 gcaccgccac atctaactga cgtgtgccat taatgggttc atagaaagcc gccgccagag
     1081 agcgtggttg atcggtgcct ttttctgcca gcgcccagga agcataggca cggctggcaa
     1141 agctattttg tttaccggtt ggggaaactt taagcgcggc ttccgtaaaa gcgcgtatgg
     1201 tctgattagc taacgcttcg tcaccgccga ggttttctac cagcagatct ttatcaatgc
     1261 agatataggt gtagaaaagt gctgaaccga atccggtttc tccgagatgc ccggcaccgg
     1321 catcttcaga ggcctggcgc aaatcatcaa cggcggtgaa aaaatcatct tcgacaatcg
     1381 tttcactgac accaaacgca tgcgcgacct ggcaggcggc ttcaacatta aactcaggtt
     1441 tattcgccag catacgacca aacatagcga tatctaccgc tatacggtct ttacgtaaca
     1501 acgcgagatc ttcctctttt ggcgcgcgct tttcttctgc cagttgatga gcaagcgctt
     1561 ttacggcgtc aaattctgcc gggctgatat gaactaattg ttcagtttcg gcgttagtga
     1621 gcggatcttt tggttttttg tcatttttag ctttcccaag ataatcagca atttttgccg
     1681 cccattcgat ggctttttta tcttcaatcc ctttctcaat caggatagtt gccgcctcac
     1741 gcgcaatacg cccactgcga ataccaatat ggcccgccag tgcctgttca aaaagtgcag
     1801 aagtgcgcca cgcacgtttc agactttgcg aggaaacgcg cagacgcgtt gctccaccca
     1861 gcaccacagt ttttggcgca ccggtgtcat cacggttaag gttggcagca gggtaagcgg
     1921 ttaacacatg aagctgaata aatgttgtca tgagatagtc ctttattgat tttgttcgtt
     1981 atcggcgtcg ccagcctggt aatattccag cgcccagcgg atacggataa attcggttgg
     2041 ccgctgctgg cggcggtgat gattgagcag gtcgtcgctc tcctggcacc agcggaaaat
     2101 gccatctgcc agggagtcaa gattgacgga accgtttaat aatctgacgg cgcggcgtaa
     2161 ctggcgcagt aattcatccg gtgtttttac tgctgacagg cgagtaaaac gcccctttga
     2221 cattaccgcc gccagttgcg cggcaaaagg ctgccgttca tcaatggctt taacattggc
     2281 gctaactgcg gctatcagcg ccagtgcggt gatacgccac tcaggttcat cctgccactt
     2341 tatttgtctg ttcttcagaa acaggcgaaa tccatccgtc agacaaacat cattaaccgt
     2401 cgtactacgc cgcagactgg cacgttcgcc gcgtttctcc tgcaattcct cgtgccattt
     2461 gcgcagcgtg gctttgtgct cctcttttac aatactcatt cagcagcctc ctgctttttc
     2521 tccctggcgg ctttagcact ttgcttctcc gccgatgttg taaaatattt cttgcgcgcg
     2581 gtcatgatgc gttccaaatc aacgggctca taaggattgg tgaatacacg ctcgtcaaaa
     2641 tcctgacgcg cgaataacca aatttcctta ttccatttgc tgagtaattc atccgcatcc
     2701 tgaccttctt caatttggcg cactaacctc aggaagcgat gctgagtttt gttccagaag
     2761 tcgatatcca caaaactaaa atcacccctt gcaccttttg gatcggagaa ccatgcttct
     2821 ttcaatgcac tccgtaacag acttagaatc cgtgaagccg tttgcgcagc cagccgcagt
     2881 ttcggtatct ggccttcttt tttattgagc agcagcggga aatgatgttc gtaccaacag
     2941 cgcgctttca tgttgtcgaa atcatagcca aatccccaca ggcccacttt tgcctgcttg
     3001 agactgctgg cattaaagag tttcaccacc agcgcgggaa gttccgtatt gttttctgac
     3061 ttacccgttt cgataaggcc taaccagtcg cgccagatta aaccgcccgg ttgtggtttg
     3121 accgagtaaa actcgccgcc ctctttaagt agtacacggt aaggcgttaa gggatgctgc
     3181 cacatggcat agttagcgcc gtaatttttg gtcgtcatca ggctcagaag cgcgtcgctc
     3241 tgctcaccgc aaatatcgca gttgccgact gtggtggtat taaaatcaat acgaatacgg
     3301 cgaggcattc cccagtacgc ctggagttta ttgacctgct catcggttac caccgcaccg
     3361 gccagttcgc tgatgcgcgt cgggccaagc caggggaaaa ccagatcgtc aaattttttg
     3421 ggtagcggta aatcggcttc atcctgcggc atgacattga tccacagttt gcgccacaag
     3481 ggagtttgtt gattaccctg atattcctgt aattcaatca gagtggtcat cggcccaccg
     3541 ccgcgtaaac cggtgcggta gcctttgcca cctgacggcg cattcaactg tagggagaac
     3601 agagctaacg cagagcaatg tgggcacagg tattccgtta caccacgctt gataaagtgg
     3661 tctttattaa acttcgttgt ttgagcgccg ggaatctcag gcagtagcga agcgaccgga
     3721 actttatctc cggtgagcgc ctcgaaatcc tgtataaatg aaggggaatc tggcccaaac
     3781 tggaaagcat gttctaatga cagcaatgct tcccgtagct tttcagcttc cagtccgtct
     3841 tcccagatat catcccaacg acgatgatct tttggcgcga agctggtttg tagtagcccc
     3901 agcaaaaact gccataccgc accctggaga tctgcccgcg gcgcagaaat atcgacaaca
     3961 ttttcatccg ccagatcgac tggcgccagt ttgcctgtcg ttccgtcttt aaaacgaacg
     4021 ggcaaccacg gggttgtcag aagtgaaaac gagttcatac gcatcctctc gtaaaaaaga
     4081 ccatcccagg tcggttgtcc atctttccat ccttttcggt gtatcaacta ttcagagagt
     4141 cctgtcgcag acatctcaac caaccttgcc aatcaatcct tccgtagccg aatacccgca
     4201 actttcgtca tcgttgacta aaataacgtt tgccatctcc ggatcttgtc gctgttcaat
     4261 gcaccacagc ctgaacgcct ctccctccag taaagaaaac tcatcccgat gttttttcca
     4321 ccagcttcgg cgcactctga caacgctcat ttcccatgcg tgagcaccgg tggcataagg
     4381 cttcaccaca ccgtcaatac aggtagccag ccacagggaa acagactcct cagccagacg
     4441 tgtcgacagt ttttctggga ggtaatcgtt gatattggcg gcatagccgg gcctgaagtt
     4501 cagcacaaac tttttagcca ttgcgcgatc acagtaatat ttgcccactt gctcctgctc
     4561 gctgcgggca aatccttccg gcattaccac gtcctcaccg tagacggatt caataagaag
     4621 ccgggctgcg tgtggcatct gaatagcacc ttgctcacgc agcacacgct gcgtcagcca
     4681 gattcgccca tgatcgggat agacaaatgc actgttacgc atggcactgc caaaccattc
     4741 gtcgccagga gagtcgtccc agacgggagc cagaatcagc aattcagggg gggaacgctc
     4801 gtcctttccg tcacgcttta actgaccatt aatatcgcgg atatgccgct gtaatcgtcc
     4861 tgctcgctga atcagcaaat caacaggggc caggtcggag atcatttcgt ccaggtcaca
     4921 atcgacactc tgctctaata cctgagtaca aataaggact tttccggcac gctgtgagct
     4981 atcttcttta ccaaagcgtg ccagtgtctc cgtttcaatt cgctggcgat cgctaaaagc
     5041 aaagcggcta tgaaaaagtg aaaggctgga agcaggaatg acgccgcggg caagcagctg
     5101 acgatgaacc ttaatagcgt catcgacaga attccggatc caggcgatgc atttcccctg
     5161 acttaccgcc gattcgatac gcgcaataca ctcttgttca ctatgaagcc aacccacgct
     5221 gacgctacgc tcaacgtctt tgcgcgtcgc tacccggtgt gagttcacat cggatttcgt
     5281 gacatgcgtc agccaggggt aatcatcctt ttccaggaac ggagcttctt gcaggccctc
     5341 tgtaccccgc gcaaaggcag cgacgagttt gtcgcgctgc tgttgagata acgtggcaga
     5401 aagcaaaata acgctgtttc cgccacgcgc ctgccgctcg atcaaccctt caagaatgca
     5461 cgacatgtaa gcgtcacagg cgtggatctc atcagccagc aggattttgt tactcagccc
     5521 cagaagccgc agattattat gtttgaacgg catcaccgcc atcatcgcct gatccagcgt
     5581 gccgacgcca atttcagcca gcaacgcctt cttgttactg tcggcaaacc aggccgcaca
     5641 tccctgactg aacgtttgtt catccggttc ttctgacccg actaaatcac cagaccagag
     5701 tgattcatta aagcggtcca ttaatgtgcg ggcactatgt gccagcacca ggctggggcg
     5761 ggattctggc gaatagaaag caagccaggt tttgaccagc cgatcgtaca tggcattggc
     5821 cgttgccatt gttggcagac caaaaaacag cccctgagcc ttccccgcag ctatcaacct
     5881 gtgcgccagg ataagcgccg cttctgtttt acctgcgcca gtcacatctt ccagaataaa
     5941 taactgcggt ccttgctggc tgatatccag atccagcacc ttttgctgta acggtgtcgg
     6001 attctcaata aaaggaaaca gcgtattaat tccgttgaaa ggcgcgactt ttgcttttag
     6061 aggaaagacg gttaaggcgt tttgagcctg aactaaagtt ttctgccagt aacctttaat
     6121 atccattggg tgtgcgacgc gtggaaaaaa tcgcgttgac gaacccgtcc agtctgcgag
     6181 cacgacggtt gcagagatat accaggaaag gtgttttata agttcaatgc cctcatcatc
     6241 atcccagaat gcggggatct ctatgagcgg aaacagtggt ttgatttcaa ggagaaaatc
     6301 tcgtgctgca gctttgtctt caggcagaaa attatccaat tcatccatac gatcaggggg
     6361 ccgaccatga tgccctgtcg tcacgggcat ccacatttct atcactcgcc taagtttgcg
     6421 cggagagagc gaagatgaag gaagtaactc ctgacattca cttaaataat gattccacaa
     6481 ccagtagccc agcgttgaat gagagatttt ttcgtaattt tttctggcac cttcctgaat
     6541 cttgagttca gggggcaggt aaagttgttg aaaagagcgg gcaaattttc caatatcgtg
     6601 ccagcacagc aaccaggcga aaaattgagc cgcctgttcc ttgtcagaaa tccctaattg
     6661 acgaaaataa tcagccagcc cgaagcaatt tcttttaacc attaaatagc ccatcgcggc
     6721 tacatccagc gaatgccagc aaagaaggtg atagtcgtcg cccccgtctt tctcgccgcg
     6781 tcgggttttt ccccagaaat caaaaaaagt cacaatattt ttatccttca gt
//
LOCUS       DAZPDU010000211         6735 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZPDU010000211 DAZPDU010000000
VERSION     DAZPDU010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6735
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.213"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..933
                     /gene="dusA"
                     /locus_tag="ACHURS_003948"
     CDS             <1..933
                     /gene="dusA"
                     /locus_tag="ACHURS_003948"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFR1239563.1"
                     /translation="WTDRHCRYFLRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEH
                     PVALQLGGSDPAALAQCAKLAEARGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVAD
                     CVKAMRDVVSIPVTVKTRIGIDDQDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGL
                     SPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQ
                     NPGILAAVDREIFGSSDTDADPVAVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIP
                     GARQWRRYLSENAHKAGADINVLEHALKLVADKR"
     gene            1067..1309
                     /gene="pspG"
                     /locus_tag="ACHURS_003949"
     CDS             1067..1309
                     /gene="pspG"
                     /locus_tag="ACHURS_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFR1239564.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(1475..2458)
                     /locus_tag="ACHURS_003950"
     CDS             complement(1475..2458)
                     /locus_tag="ACHURS_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFR1239565.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREEDLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            2541..3956
                     /gene="dnaB"
                     /locus_tag="ACHURS_003951"
     CDS             2541..3956
                     /gene="dnaB"
                     /locus_tag="ACHURS_003951"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR1239566.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4009..5088
                     /gene="alr"
                     /locus_tag="ACHURS_003952"
     CDS             4009..5088
                     /gene="alr"
                     /locus_tag="ACHURS_003952"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFR1239567.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSIGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            5341..6534
                     /gene="tyrB"
                     /locus_tag="ACHURS_003953"
     CDS             5341..6534
                     /gene="tyrB"
                     /locus_tag="ACHURS_003953"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFR1239568.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVDADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT         1556 a         1680 c         1816 g         1683 t
ORIGIN      
        1 tggacggaca gacattgccg ctatttcttg cgtctgcttt cccgcaatac gttgctgtat
       61 accgaaatgg tgaccacagg ggcgattatt cacggtaaag gtgattacct ggcgtacagt
      121 gaagaagaac atccggtagc gttgcaactc ggcggtagcg atccggcggc gctggcacag
      181 tgtgcgaagc tggcagaagc gcgtggatat gatgagatca acctgaatgt cggctgcccg
      241 tctgaccggg tgcagaacgg catgtttggt gcgtgtctga tgggtaatgc gcagctggtt
      301 gccgactgcg tgaaagcgat gcgcgatgtg gtgtcgattc cagtgacggt gaaaacgcgt
      361 attggcatcg atgaccagga cagctatgaa tttctctgcg atttcatcaa caccgtttcc
      421 ggcaaaggcg agtgtgagat gtttatcatc cacgcacgta aagcctggct ttcggggtta
      481 agcccgaaag aaaaccgtga aatcccgccg ctcgattatc cgcgtgtgta tcaactgaag
      541 cgtgactttc cgcatctgac aatgtcgatt aacggtggta tcaagtcgct ggaagaggcc
      601 aaagcacacc tgcaacatat ggatggcgtg atggtcgggc gcgaggcgta tcagaatccg
      661 ggtattctgg cggcggtaga ccgggagatc tttggttcct cggataccga tgccgatccg
      721 gtggcggtag tgcgcgccat gtatccgtac attgagcgtg aactcagcca ggggacgtat
      781 ctcggccata ttacccggca tatgttgggc ttgttccagg gtattcctgg cgcgcggcag
      841 tggcggcgtt atttaagtga aaatgcccat aaagcgggtg cagacattaa tgtgctggaa
      901 cacgcgctca aactggtggc ggataagcgt taacttttca ccaaaaagta gtcaaattca
      961 ccacgccctg cgcaccgtcg cggggcgttt tgctgttaaa tcaatagatt atttttggca
     1021 tgattcttgt aatgccagca agagatttca tatttgggag agcatcatgc tggaactact
     1081 ttttgtgatt ggcttttttg tcatgctgat ggtcaccggc gtttcgttgc tgggcattat
     1141 cgccgcgctg gttgtggcga cggccattat gttcctcggc ggtatgctgg cattgatgat
     1201 taagttgctg ccgtggttac tactggcgat tgcggtggtg tgggttatta aggcgattaa
     1261 agcaccaaaa gtgccgaaat atcagcgtta tgaccgctgg cgttactaag ggattgtgcg
     1321 gatgatcaca acctaaggtt ttatccttag aacaaaatag gaattgataa tcaaatctgt
     1381 cactattgcg cctctaacag attcatcgtg ctgtacccta catacagccg aactataaaa
     1441 agaaagggct tcccaggtgg aagccctatt tcttttatgg aatcaacagg ctggaacctt
     1501 gcgtcgcccg gctttccaga atctcatgcg cacgctgcgc atccttcagc ggatatttct
     1561 gctgctcggc gacatcgacc ttaatcacac cgctggcaat caaagagaac agttcattac
     1621 tggcctcggt taattcctcc cgcgtggtga tatagccttg cagggaaggg cgtgtcacat
     1681 acaacgagcc tttttgattg agaatgccta agttcacacc ggtaaccgca cctgatgagt
     1741 tgccaaaact gaccattaag ccgcggcgtt gcaggcaatc cagcgaccgt tcccaggtgt
     1801 ctctgcccac ggaatcgtac accacgcgca ctttcttacc gccggtgatc tcttttaacc
     1861 gctcgaccag atcctcttca cgatagttaa taacctgcca cgcgcccgct tttagcgcgc
     1921 tctgcgcttt ttgcgcggtt cctacggtgc cgataagttt cgcgcccagg gcttttgccc
     1981 actggcaggc aattaagcca acgccgccag ccgctgcgtg gaacaggaac tgttcatcgg
     2041 gtttaatttc ataggttttg cgcagcagat aataaaccgt taagcctttc aggaaggatg
     2101 ccgcagcttg ctcaaaagaa attgccgcag gcagaatcgc cgctttatcc gcattaatgt
     2161 tatgcacaga gctgtaagcg cctaacgccg actgcgcata gactacacga tcgcctgcct
     2221 taatatgctt tacaccactg ccgactttac tcacgatgcc tgccgcttcg gtacctaatc
     2281 cgctgggtag tgatggcggc gggtaaaggc cgctgcggat atacgtgtcg ataaaattga
     2341 tgccgatggc tttattttcg acctggattt cattctctgc cggatcggca ggagtgaact
     2401 ctacggcttg aagtacttcc gggccaccgt gcttgtgaaa ttcaattcgt gttgccatgt
     2461 gtcctccaga acgtatcgtc agggtctgct tcatatggta aagtttcgac ccattcttta
     2521 tctcggtaac tccattcact atggcaggaa ataaaccctt caacaaacag caggctgaac
     2581 cccgcgaacg cgatccacaa gttgccgggc tgaaagtgcc tccgcactcg atcgaagcgg
     2641 agcagtcggt gttgggcggt ttaatgctgg ataacgaacg ctgggacgat gtagccgagc
     2701 gtgtggtggc agacgatttt tacacccgcc cacaccgcca tatctttact gaaatggcgc
     2761 gtttgcagga aagcggtagt cctatcgatc tgattaccct tgcggaatcg ctggaacgcc
     2821 aggggcaact cgatagcgtc ggtggttttg cttatctggc agagctgtca aaaaatacgc
     2881 caagtgcggc gaacatcagt gcttatgctg acatcgtgcg tgaacgtgcc gttgtccgtg
     2941 agatgatctc ggttgcgaat gagattgctg aagccggttt tgatccgcag gggcgtacca
     3001 gcgaagatct gctggacctt gctgaatccc gcgtctttaa aattgccgaa agtcgtgcaa
     3061 acaaagacga agggccgaag aacatcgccg atgtgctcga cgcaaccgtg gcgcgtattg
     3121 agcagttgtt tcagcagcca cacgatggcg ttaccggagt aaacaccggt tatgacgatc
     3181 tcaacaaaaa aaccgctggc ttgcagccgt cggatttgat catcgtcgcc gcgcgtccgt
     3241 cgatgggtaa aacaacattt gcgatgaacc tcgtcgaaaa cgcggcgatg ttgcaggata
     3301 aaccagtact tatcttctcg ctggagatgc cttcagaaca gattatgatg cgttctctgg
     3361 cgtcgctgtc gcgcgttgac cagactaaaa tccgtaccgg gcagctcgat gatgaagact
     3421 gggcgcgcat ttccggcacc atgggtattt tgctcgaaaa acgcaatatc tatatcgatg
     3481 actcctccgg cctgacgcca acggaagtgc gttcccgcgc acgccgtatt gcccgtgaac
     3541 acggcggcat cgggcttatc atgatcgact acctgcaact gatgcgcgta ccggcgcttt
     3601 ccgataaccg tacgctggaa attgcagaaa tctctcgctc gctgaaagca ctggcgaaag
     3661 aactgaacgt gccggtggtg gcgctgtccc agttgaaccg ttctctggaa caacgtgccg
     3721 acaaacgccc ggtcaactcc gacctgcgtg aatctggctc tatcgagcag gatgcggact
     3781 tgattatgtt tatctatcgt gatgaggtgt atcacgaaaa cagtgattta aaaggcatcg
     3841 cggaaattat tatcggtaaa caacgtaacg gcccaatcgg gacggtacgc ctgaccttta
     3901 acggtcaatg gtcgcgcttc gacaactatg cggggccgca gtacgacgac gaataataat
     3961 tattttatga attaggtaat taaagcaaac acttatcaag gaacacaaat gcaagcggca
     4021 actgttgtga ttaaccgccg cgctctgcga cacaacctgc aacgtcttcg tgaactggcc
     4081 cctgccagta aaatggttgc ggtggtgaaa gcgaacgctt atggtcacgg tcttcttgag
     4141 accgcgcgaa cgctccccga tgctgacgcc tttggcgtag cccgtctcga agaagctctg
     4201 cgactgcgtg cggggggaat caccaaacct gtactgttac tcgaaggctt ttttgatgcc
     4261 agagatctgc cgacgatttc tgcgcaacat tttcataccg ccgtgcataa cgaagaacag
     4321 ctggctgcgc tggaagaggc tagcctggac gagccggtta ccgtctggat gaaactcgat
     4381 accggtatgc accgtctggg cgtaaggccg gaacaggctg aggcgtttta tcatcgcctg
     4441 acacagtgca aaaacgttcg tcagccggtg aatatcgtca gccattttgc gcgcgcggat
     4501 gaaccaaaat gtggcgcaac cgagaaacaa ctcgctatct ttaatacctt ttgcgaaggc
     4561 aaacctggtc aacgttccat tgccgcgtcg ggtggcattc tgctgtggcc acaatcgcat
     4621 tttgactggg tgcgcccggg catcattctt tatggcgtct cgccgctgga agatcgctcc
     4681 atcggtgccg attttggctg tcagccagtg atgtcactaa cctccagcct gattgccgtg
     4741 cgtgagcata aagccggaga gcctgttggt tatggtggaa cctgggtaag cgaacgtgat
     4801 acccgtcttg gcgtagtcgc gatgggctat ggcgatggtt atccgcgcgc cgctccatcc
     4861 ggtacgccag tgctggtgaa cggtcgcgaa gtaccgattg tcggacgcgt ggcgatggat
     4921 atgatctgcg tagacttagg tccacaggcg caggacaaag ccggggatcc ggtcatttta
     4981 tggggcgaag gtttgcccgt agaacgtatc gctgaaatga caaaagtgag cgcttacgaa
     5041 cttattacgc gcctgacttc aagggtcgcg atgaaatacg tggattaatc gttctgtaat
     5101 atttgattgt ctgtgccgga tgcggcgtga atgccttatc cggccaataa aatcctaaaa
     5161 attcaataag ttgatgttct ttcatgctct tataaaggtc gtgcctctgg cggatgttcg
     5221 tttgtcatga gtctcactct gttgctaatt gccgttcgct cctgaacatc cactcgatct
     5281 tcgccttctt ccggtttatt gtgttttaac cacctgcccg taaacctgga gaaccatcgc
     5341 gtgtttcaaa aagttgacgc ctacgctggc gacccgattc ttacgcttat ggagcgtttt
     5401 aaagaagacc ctcgcagcga caaagtgaat ttaagtatcg gtctgtacta caacgaagac
     5461 ggaattattc cacaactgaa agccgtggcg gaggcggaag cgcgcctgaa tgcgcagccc
     5521 catggcgctt cgctttattt accgatggaa gggcttaaca gctatcgcca tgccattgcg
     5581 ccgctgctgt ttggtgcgga ccatccggta ctgcaacaac agcgcgtagc aaccattcaa
     5641 acccttggcg gctcaggggc attgaaagtg gacgcggatt tcctgaaacg ctacttcccg
     5701 gaatcaggcg tctgggtcag cgatcctacc tgggaaaacc acgtagcaat attcgccggg
     5761 gctggattcg aagtgagtac ttacccctgg tatgacgaag cgactaacag cgtgcgcttt
     5821 aatgacctgt tggcgacgct gaaaacatta cctgcccgca gtattgtgtt gctgcatcca
     5881 tgttgccaca acccaacggg tgccgatctc actaatgatc agtgggatgc ggtgattgaa
     5941 attctcaaag cccgcgagct tatcccattc ctcgatattg cctatcaagg atttagtgcc
     6001 ggtatggaag aggatgccta cgctattcgc gccattgcca gcgctggatt acccgctctg
     6061 gtgagcaatt cgttctcgaa aattttctcc ctttacggcg agcgcgtcgg cggactttct
     6121 gttctgtgtg aagatgccga agccgctggc cgcgtactgg ggcaattgaa agcaacagtt
     6181 cgccgcaact actccagccc gccgaatttt ggtgcgcagg tggtggctgc agtgctgaat
     6241 gacgaggcat tgaaagccag ctggctggcg gaagtagaag agatgcgtac tcgcattctg
     6301 gcaatgcgtc aggaattggt gaaggtatta agcacagaga tgccagaacg caatttcgat
     6361 tatctgctta atcagcgcgg catgttcagt tataccggtt taagtgccgc tcaggttgac
     6421 cgactacgtg aagaatttgg tgtctatctc atcgccagcg gtcgcatgtg tgtcgccggg
     6481 ttaaatacgg caaatgtaca acgtgtggca aaggcgtttg ctgcggtgat gtaatgcagg
     6541 aaagcaggct ggagttaccc agcctgcagt gaaattaaac tgtcgtcgct ttcactattt
     6601 ctttgtagat gatttttttg atgccgttgt tctacgtgag agataataaa acgttgtcag
     6661 ttcttttatt gttaagttta tcccaattat ctggaattcc tttccctatt ttttcgtgtg
     6721 gagtaataat ttctg
//
LOCUS       DAZPDU010000212         6730 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZPDU010000212 DAZPDU010000000
VERSION     DAZPDU010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6730
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.214"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..1094)
                     /locus_tag="ACHURS_003954"
     CDS             complement(<1..1094)
                     /locus_tag="ACHURS_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HFR1239569.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVLHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWSLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDATLVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPQ
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSN"
     gene            complement(1131..2576)
                     /gene="abgB"
                     /locus_tag="ACHURS_003955"
     CDS             complement(1131..2576)
                     /gene="abgB"
                     /locus_tag="ACHURS_003955"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HFR1239570.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLRGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            complement(2576..3886)
                     /gene="abgA"
                     /locus_tag="ACHURS_003956"
     CDS             complement(2576..3886)
                     /gene="abgA"
                     /locus_tag="ACHURS_003956"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HFR1239571.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            4062..4970
                     /locus_tag="ACHURS_003957"
     CDS             4062..4970
                     /locus_tag="ACHURS_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1239572.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            5300..5863
                     /gene="smrA"
                     /locus_tag="ACHURS_003958"
     CDS             5300..5863
                     /gene="smrA"
                     /locus_tag="ACHURS_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFR1239573.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQVKQENWERHAKRSR"
     gene            complement(5884..>6730)
                     /gene="dgcM"
                     /locus_tag="ACHURS_003959"
     CDS             complement(5884..>6730)
                     /gene="dgcM"
                     /locus_tag="ACHURS_003959"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFR1239574.1"
                     /translation="FYARFFLTNSAPMLLIDPSRDGQIVDANLAALNFYGYNHETMCQ
                     KHTWEINMLGRRVMPIMHEISHLPGGHKPLNFIHKLADGSTRHVQTYAGPIEIYGDKL
                     MLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQMQHLAIAQDYSLLL
                     IDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGEEFVLLLPRTPLDT
                     ALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRVDDALYRAKNDGRN
                     RVLAA"
BASE COUNT         1685 a         1715 c         1704 g         1626 t
ORIGIN      
        1 atgttgctcc agttaaacat ggcgacaaac tgggcgaggg gaaaaaccat cacgataaat
       61 cctgccatct ctttcatcgg ttcaatcatt aactgcggta aatccgcctg acgtcgaatt
      121 gtgcgggtag cgatgccata agccagcgag acaacaaaga aaaaaagaat gatcagtggc
      181 acgatacctt taataaaggg tgatggcatc acggtgtgat taatcggatc gcgcaatatc
      241 ccgttttccg ggatcaccat cagcgcaatc gcagcaataa aaagtagcga tacgacacct
      301 gctatgcgta aaccaaaacg ctgactttcg gtcaatgtct gcagtttctc atcgctgttt
      361 ccctgccatt gacctaaccg tggctcgatg attttgtcgg ttatcaggcc gccaacaatc
      421 gtcagtacga ctacggagct ggccataaaa taccagttat caattacact gacgtgcatt
      481 tgcggattga acgcagccgc cgcttccgtg ctgatccccg acagcaacac gtcggttgtg
      541 acaatcagta aattagccgt aaagccgcaa cctacgcctg caatggcagc cagtaaacct
      601 gcaactggat gcctgcccac cgccagaaaa atcagcgcac ccatcggtgg catgatcact
      661 aacgtcgcat cggaagaaat gtggctgaaa aaagcaataa acagcaccat ataactggcg
      721 tagcgggcat taacatgcga tgccatttta accattagcg ctggcagtaa gccgacgcgc
      781 tccgccagac cggcacctaa aaccagcgcc aggatcgcac caagtggagc aaaaccgcta
      841 aagtttttaa taacattggg taaagaccag tgtaatcctt ccacactgag caggtttttc
      901 accaccaccg gcgttccatc ggtcgggttt ttcgcactga cgccaaaggc cgacaaaatt
      961 gccgtcgtca ccatgagtac gataatcaaa tagataaaga gcaaaaaagg atggagaacc
     1021 ttgttaccaa ttctttcgac ccagccatag agcttcccgg attgggagga cgacggtatg
     1081 gatgacatac tcatgggcat tcctcggttg ttgtgtttgc gttgttgttg ttattttaaa
     1141 ggtgacggtg tcacgttttt cgggataggg cagtgatacg gttgcgtgtc cgttacttgc
     1201 tgatgctctt gttggcattc ttgcaatagc cctgaatcaa tgaagagatt gactgtggtt
     1261 gctgccatag ttttcgccgc cagcagcatt cctttatgag caatagatgt tcgcccctgg
     1321 ctaaccagtt gccacgtatg tagcggtgta ccgacggcaa aacaggggct gaaacactgg
     1381 gcaacaggca gtttccaact aacgtcgccg acatcagtcg atgccgcaag cacgttatcg
     1441 gtggcggcat atggagcgac ctcattcgcc agtaccgttt cacgatgacg tagtgcaaaa
     1501 gccttgccgt tttcgccacc ggttgcggcg atattattca gactgttttg ccgatcgttg
     1561 ggggtgagcg tagcctgaat ttgtttcgca aaagccagtt cttcggagtt ccattccggg
     1621 gtaccaaaat gggatagggc atggtacatg gcattttcta aggtgcgatt cgggagataa
     1681 ctggaacagg ctttgtcgaa gcggcattca accgtggttt cggtcatcaa tgccgcacct
     1741 tcggcgattt tggcgacccg atcataaata tgctgcacgt cggtcatttc gggggcgcgg
     1801 ataagataaa gcacttctgc ctgcgcctgg accacgttgg gcgagatccc gccgctattt
     1861 gtgatggcat agtgtacgcg cgctttttca ataatatgtt cgttgaggaa gttggtgcca
     1921 gtggtcatca acgttacggc atcaagggcg ctgcgtccca aatgagggga attcgcggca
     1981 tgtgctgcga tccctttaaa gcgccatgat gcctgaatgt ttgccagcgt gcgggtattg
     2041 aacataccgg caaaggcttc cgggtgccag gtgagtgccg catccacatc atcaaatacc
     2101 ccctcgcgga ccatgaacgt tttacccgag ccgccttctt cgccaggaca accataaaag
     2161 cgcactgtgc cgccttgccc atattgttcc agccatttct tgacggctat tgcagcggca
     2221 aaggcggcgg ttccccgcaa attgtgtccg caaccgtgac cattttcacc gggcgtcgcg
     2281 gatgtaggtt gcgcgcaacc agcttgctga cttaaacctg ccagggcgtc atattctccc
     2341 agcagggcga taaccggttt gccttgacca aacgaagcaa taaaggcatt tgggatattg
     2401 cctacgttgc gggtaacggt gaagcctgca gattccagag ccgaagccag atgctccgct
     2461 gaccagaact cttcaaaacg tgtttctgga tgatcccaga tttgatcggc aatatcggta
     2521 taacgttggc gatcggcttc aatcgcatcg tcgataaaac gatagatttc ctgcatcaga
     2581 tacctcgcgt ccagggaaaa ttgagcgcgg tgcgcgccag cgtttcgacg gcaatagcga
     2641 gaacctgctc gtcaaaatcg aatttttcgt tgtgatgacc tgccgccagc tgtgtgccaa
     2701 acaccacgta ggaggcttgc ccttgatgtt gctgcacgcg ggccatcatt aatgtggcat
     2761 cttcggaacc cgcaggcgct tcaacacgtt caatggcctg attgaccccc gcgacctgag
     2821 ccgcctgact ttgcaaccat gcgacccatt gcggcgaagg agaactggcg gtagctgcac
     2881 ccatcagacg agtttcaacg ccgacaccat acatggttgc tgcgccctga atcgcttgtt
     2941 gtgcacggtc aaaaacatat tgattaatga cgtcgctggc cccgcgtgtt tccactttca
     3001 gcaacgccga ggcaggaaca acgttacgac cgcttcctgc ctgcataacg cccacgttta
     3061 ctctggaagc tccttcgctg tgcggggcga ttgcatgcag tgcaagagtg gcttgtgctg
     3121 ccgccaacaa ggcattgtga ccgtcttctg gttttgcgcc tgcgtgagcg gcggtaccgg
     3181 tgaagtgcgc gtcaaatttg gtggttgcca taaaattatc actgccgcac accacggtgc
     3241 ccgcaggtac gccagtgcca atgtgcacgg cagtaaaata atcaacatca tctacgacac
     3301 ctgcatcgac catcgcccgc gcgccacgcg taccttcctc tgcaggctga aaaatcagtt
     3361 tgatgacgcc atgtagtccg gactcgaatt gtttaagggt atgcgccagc ccaagcccaa
     3421 tggcggtatg tccatcatga ccacaggcat gcatcattcc ggcgttacat gacgcaaaac
     3481 cgtcgcggta ggggcgatgg ctgacatcct gctcttcact gagatccagc gcgtccatat
     3541 cgacacggaa agccatcacc ggaccggggc gaccggtatc cagggtggcg acgataccag
     3601 tgaaaccacc ttcaaaaacc gcaatccatt gtgctagcgc accctgttga cgagcgcgct
     3661 cgaattcgcg ttgtagagtg agttcatcag gtagtcccat ccggctactt tcattaacta
     3721 cttcgcggcc cagcgccagt gaatagccga gctgctgcaa ttcttccgca acaagggtgg
     3781 cagtgcggaa ttccacccag ccagactctg catagtgatg aaaatcacgt cgccagtgcg
     3841 ataattttgg ggcaagcgaa ttaacaaatt gattcaaaga ctccataacc tttcccgtca
     3901 tcagtaaaaa gtgtgacccg gttcacgtag cgatagtttt tacttatcac taactgattt
     3961 ttcacagttt taaccgttca taaattaccc tgacacaatc atctgcatta aagtagatgc
     4021 cagtttcttt ggtctgataa ataacggtta tcggtggcgt catggctttt caggtaaaaa
     4081 ttcatcaaat tcgggctttt gttgaagtgg ctcgtcaggg cagcattcgc ggagcgagcc
     4141 gaatgttgaa tatgtcgcaa ccggcactga gtaaatctat tcaggagctg gaagaaggat
     4201 tagcggcgca actctttttt cgccgtagta aaggcgtgac gttaactgat gccggtgaaa
     4261 gtttttatca gcacgccagt cttattcttg aagagctgcg cgcagcccaa gaggatattc
     4321 gccaacgaca agggcaactg gcagggcaga ttaatatcgg catgggggcc agtatttccc
     4381 gcagtctgat gccagctgtc atatctcgtt ttcatcagca gcatccgcag gtaaaagtac
     4441 gcattatgga agggcaactg gtgtcgatga ttaatgaatt gcgtcaggga gaattggatt
     4501 tcaccatcaa tacctattat cagggaccgt acgaccacga atttactttt gagaaattac
     4561 tggaaaagca attcgcgatc ttttgccgcc cgggacaccc cgccattggt gcccgttcga
     4621 tcaaacagtt actggattac agctggacaa tgccgacgcc acacggcagt tactacaaac
     4681 agttgagtga attgcttgac gatcaggcgc aaacgccaca ggtcggtgta gtctgcgaga
     4741 cgttctcagc ctgtatcagt ctggtggcaa aaagcgattt tctcagcata ctgcctgaag
     4801 aaatgggctg cgatcccttg cacggacagg ggctggtgat gttgccggtt agcgaaattt
     4861 taccgaaagc ggcctattat ttgattcagc ggcgtgatag tcgccagaca ccactgaccg
     4921 cgtcattaat cacgcaattc cggcgagaat gcggctatct gcaaagttaa aactgcataa
     4981 aaaaatagag tctgtcgaca tccgccagac tctacagtac acacagcagt gcatccgcgt
     5041 cttaaatccg gcattgtctc ctctgcgccg gtgactgtgt tataacggtt ttaaccttta
     5101 gttgccaatt ttcttcagtg acagatttca cgaaaatata agttacatta ttaatatcgt
     5161 gaatgaataa tcatgcataa gcattttgct taaaatatcg gcaatatttg gaacttatta
     5221 ctggaatttt gggtaatacg ttgttggacc gacccggtct ggttatcata tcgcgctctt
     5281 aattgcggga ggatgtaaca tgaaccttga cgacaaatcg ctgtttcttg acgccatgga
     5341 agatgtccag ccgctgaaac gtgctaccga tgtccactgg catccaacgc gtaaccaacg
     5401 tgcgccgcag cgtatcgaca cgctgcagct tgataatttc ctcaccaccg gatttctcga
     5461 catcatccca ctaagtcagc cgctggagtt tcggcgggaa gggttgcaac atggggtgct
     5521 ggataagctg cgcagtggta aatatccgca acaggcgagc ctgaatcttt tgcgccagcc
     5581 ggtggaagag tgccgcaaaa tgatgttcag tttcattcaa caagccatgg ctgatggttt
     5641 gcgtaacgtg ctgattattc atggcaaagg acgggatgat aaatcgcatg ccaatattgt
     5701 ccgcagctat gtggcgcgct ggctgaccga atttgatgat gttcaggcat attgcactgc
     5761 gctaccgcat catggcggca gcggggcgtg ttacgtcgca ctacgtaaaa cggcgcaggt
     5821 gaagcaagaa aactgggagc gccacgctaa gcgcagtcgt tgatctcgag acgcatccgc
     5881 ggcttatgcc gccagcacgc ggttgcgtcc atcatttttc gcccgataca aagcatcatc
     5941 aacgcgttta aacagttcat cgatgctttc atttccttcg tgatgcgcca caccaatgct
     6001 gacggtaaag cgtggtaagc ccgaaatact cacttttgcc acgcttacgc ggatagtttc
     6061 agccagcgaa agcgcggtat ccagtggggt tcttggtagc aataagacaa actcttcgcc
     6121 tccccaacga aacaccaaat cgcctttgcg agcgcaactt tcgagggtgc gggcgagggc
     6181 gcataacacc tcatcacctt tagaatgccc atagagatcg ttaatgtgtt taaaacgatc
     6241 ggtgtcgatg agcaacaagc tgtaatcttg ggcgatggcg agatgctgca tttggcctgg
     6301 ctcggtaatg tgataaaact gtcgccgatt cagtaatccg gtcatcgcgt catggtgggc
     6361 agcatgttcc agctgctcct ccagccgttt ttgctcagta atatcatgta caatacataa
     6421 catgagcttg tcgccataaa tttcaatcgg cccggcgtag gtctgcacat gacgagtcga
     6481 accatccgcc agcttatgaa taaaattcag aggtttatga ccaccgggta aatgcgaaat
     6541 ttcatgcatg ataggcatga cgcgacgccc gagcatattt atttcccagg tatgtttctg
     6601 gcacatcgtt tcatggttat aaccatagaa attgagcgcg gcgaggttag catcgacgat
     6661 ttggccatct cgtgaggggt caatcaacaa cattggtgca gagttagtca gaaaaaagcg
     6721 cgcataaaaa
//
LOCUS       DAZPDU010000213         6730 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZPDU010000213 DAZPDU010000000
VERSION     DAZPDU010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6730
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.215"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            38..250
                     /locus_tag="ACHURS_003960"
     CDS             38..250
                     /locus_tag="ACHURS_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239575.1"
                     /translation="MSNTQKIINTEKYNEWVKKFSEQIFKITGDENVAKNELEPWTPE
                     GNAPNYCWWEVDPVDAANEAMSYHND"
     gene            283..501
                     /locus_tag="ACHURS_003961"
     CDS             283..501
                     /locus_tag="ACHURS_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFR1239576.1"
                     /translation="MTTLFRKEYPQKSRATEFLFLILFIVLMIPISPLIFVWAIGKII
                     EPVTELYTDVVWASFNTLHNKINPYKEN"
     gene            572..889
                     /locus_tag="ACHURS_003962"
                     /pseudo
     CDS             572..889
                     /locus_tag="ACHURS_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(922..1560)
                     /gene="ompW"
                     /locus_tag="ACHURS_003963"
     CDS             complement(922..1560)
                     /gene="ompW"
                     /locus_tag="ACHURS_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFR1239577.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDAGSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDYWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            1800..1889
                     /locus_tag="ACHURS_003964"
                     /pseudo
     CDS             1800..1889
                     /locus_tag="ACHURS_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            1917..2660
                     /locus_tag="ACHURS_003965"
     CDS             1917..2660
                     /locus_tag="ACHURS_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFR1239578.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            2690..3229
                     /locus_tag="ACHURS_003966"
     CDS             2690..3229
                     /locus_tag="ACHURS_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFR1239579.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            3334..3732
                     /gene="yciA"
                     /locus_tag="ACHURS_003967"
     CDS             3334..3732
                     /gene="yciA"
                     /locus_tag="ACHURS_003967"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFR1239580.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(3772..4491)
                     /gene="tonB"
                     /locus_tag="ACHURS_003968"
     CDS             complement(3772..4491)
                     /gene="tonB"
                     /locus_tag="ACHURS_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFR1239581.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            4582..6102
                     /locus_tag="ACHURS_003969"
     CDS             4582..6102
                     /locus_tag="ACHURS_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR1239582.1"
                     /translation="MRTVITYLRFSSAIQGAEGADSTRRQNDLFKQWLKKNGDAQIVA
                     SFSDEGLSGYKGKHLTGQFGDMLARIEAGEFPEGTILLVESIDRIGRLEHLETEALMN
                     RILGNGIEIHTLQDGLIYTKDALADDLGISIIQRVKAYIAHQKSKQKSFRVSQKWGQR
                     AKLALAGEQRLTKMVPGWIDPETFKLNEHAETVRLIFKLLLDGESLHNIARHLQSNGI
                     KSFSRRKDANGFSVHSVRTILRSETTIGTLPASQRNDRPAIPNYYEGVVDIPTFNKAQ
                     EILDKNRKGRTPASDNPLTINIFKGLFRCQCGASVHPTGTKNKYAGVYRCNNHLDGRC
                     DVPPLKRKPFDRWMIDNFLGMIDVGNDGESERKIAALQHEVEIVTARIKKATALLLEM
                     DDIDELKIQLKELNQKRTELQTTIDNMRRKASLTDKELPQLKDIDLMTKAGRVECQLI
                     LSKHLKGLTLGKDSVTVTLQNDTEITIPTNPLPLNDGSPIFEIADKELLDIDAYQL"
     gene            complement(6135..>6730)
                     /locus_tag="ACHURS_003970"
     CDS             complement(6135..>6730)
                     /locus_tag="ACHURS_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HFR1239583.1"
                     /translation="RAPKTISISGVVVVDSTGSVLLTRQGQLVETFISTVEQWRDQKQ
                     IITIVCRDGIAITDSIITQFKASKEHTIANGLRIQMTFQGVEFRSIVGQTDVSAATGK
                     TATTNDGGATSKKNTGNTTTSLGSPMLTCKELFGYSASELSDEALKARVTCSKSVSVK
                     NGESTFSDAANEQASKVLKPGNALRKYSVNPNKKGTY"
BASE COUNT         1774 a         1538 c         1589 g         1829 t
ORIGIN      
        1 tgtaaataaa atttaatcct taactggagg tatatctatg tcaaatacac agaaaattat
       61 taacactgaa aaatataacg agtgggtgaa aaaattctct gagcagattt ttaaaattac
      121 tggcgacgag aatgtggcaa aaaatgaatt agaaccgtgg acacctgaag gaaacgcacc
      181 aaattattgc tggtgggagg ttgatccggt tgatgctgca aatgaagcca tgagttacca
      241 caacgattaa tgtcgggagg ccgcccgaaa gggcggtaag aaatgactac attattcaga
      301 aaagaatatc cgcaaaaaag tagggcgaca gaatttttgt ttctcattct gtttatcgta
      361 ttgatgatac cgatatcccc tctaattttt gtctgggcaa tcgggaaaat aattgagcca
      421 gttactgaat tgtataccga cgttgtatgg gcgtcgttca acacactgca caataaaatt
      481 aatccgtata aggaaaactg atatggcaac tttgacaaaa aaagaacggg catggttgaa
      541 cgaattacag gaagttcttg atcgctgtcc tgtggtgtca tcctgccgcg attgcgttct
      601 tagtccgtgg ctattgcacc accatcacgc gaaagggaca gctaagcgcg gcgggatggt
      661 taagccagca acattaaccg ttgcatttaa aaaagcccgg gattctgtgg attacaactg
      721 gcgtgctaat ggcaccccac cctctttcca tgagcagaga tctttatcag agcgattgtt
      781 cagagagcag ggggttgata ccaaaatttt gctaggccat tcgaatcaaa aaatgaccga
      841 tatttacaac gatgtacgcg gtaaggagtg gaaaaaactg gtcatttgat aaccagtttt
      901 gcagaggttt tgcagagaaa attaaaagcg gtacccggca gagaacataa acacccacgg
      961 atccagtcgt accgagtctt ttacggtagt aacaccagat ttgtacttag ccgtggtatc
     1021 gatatccatg taccacactg acatgttaac caaccagtca cggttaatca gataatcaac
     1081 ccccacctgc ccggcagctc cccagtaatc tttcagactg agatcggaaa gccccgcctc
     1141 tttgccatga tcgttaaatc cattatcaaa gaaggtggtg tagttaatac ctgccccaac
     1201 ataaggacgg aatttgctgc cggcatcacc aaaataccac tgcgccatca gtgttggcgg
     1261 cagatgatga acggttgcaa tatcgccggt cgcccgggtg ccgattttat ggcggaacgg
     1321 cgtcgctgcc agtaattcca caccaatgtt gtcggtcgcc atataagtaa acgtcaggcc
     1381 cagttgcgtg ttattggtca cgctgaatcc acccagactt cctaacgtac caccagcacc
     1441 ttctgttgga cgtacggttg cagaacctgc acgcataaaa aattcgcctg cttcatgcgc
     1501 aaaggcactg ccagagagaa gagttgttac tgccaaagcc gccactgtta actttttcat
     1561 atccgctccg tcgttatggt tataaaaacg tgacgaatat acctacattg gagtaacaaa
     1621 tgatcctaca tagatcacat tacatatgat aattcaccct ttattgatct ggattaattt
     1681 agaagttgca ttgagaatcc tggttaacgt gatttaaatc aatttttaga tattttcgct
     1741 acgcaaatat taactcgttc gccacttgcc gcggtttgcc cacgcctgaa ggccagcaat
     1801 tacctccgct ttacaaagat atgcttttcc atacaagacc tgatgctgcc acccgaggct
     1861 taaccagggt acaattgccc gctaattaac acctgcataa aactcaagga gagtgcatgt
     1921 ctatcacggc gcagtccgta taccgtgaca ccggaaattt cttccgtaat caatttatga
     1981 ccattctgtt ggtatcgttg ctatgtgcgt ttatcacagt ggtgttaggg catgttttct
     2041 cacccagtga tgcacagctt gcgcagctca atgacggcgt gcccgttagc ggcagtagtg
     2101 ggttgttcga cctggttcag aatatgtcac cggaacaaca acaaattttg ctgcaggctt
     2161 cagcggcgtc cactttttca ggattaatcg gtaacgccat tctcgccgga ggcgtaatat
     2221 taattatcca gctggtgtct gcgggtcaga gagtcagtgc gctgcgagct attggtgcca
     2281 gtgcgccgat attgcccaag ttatttattc tgatttttct gactaccctt ttagtacaga
     2341 ttggcatcat gctggtggtc gttccgggaa ttatcatggc cattttactg gccctggcac
     2401 cggtgatgct ggtacaggac aaaatgggca tttttgcctc gatgcgtagc agtatgcggc
     2461 tgacttgggc gaatatgcgt ctggtggcac ccgcagtact gagctggttg ctggcaaaaa
     2521 cactgttgct gctttttgcc tcttcttttg ccgcattaac cccggaaatt ggtgccgtac
     2581 tggcgaacac cttgagcaac ctgatttcag ccatattgct catctatctg ttccgcctgt
     2641 atatgttgat tcgccaataa ccttaagcat ttgattacgg aatcgtaaaa tgaagcagtt
     2701 tcttgatttt ttaccgctgg ttgtcttttt cgcgttttac aagatttatg acatctatgc
     2761 ggctactgcg gcgctgatcg tcgccacggc gattgtgctt atctatagct gggttcgctt
     2821 tcgtaaggtt gagaagatgg ccctgatcac ttttgttctg gtggtcgtct tcggtggctt
     2881 gacgctgttc ttccacaatg atgagtttat taaatggaag gttacagtca tttatgctct
     2941 gtttgcggga gccctgttag tcagccaatg ggtgatgaaa aagccgctaa ttcagcggat
     3001 gctgggtaaa gaactcacgc tgccgcaacc ggtatggtcg aagctaaatc tggcctgggc
     3061 tgttttcttt atcctttgcg gtctggcaaa catctacatc gcattctggc tgccgcaaaa
     3121 tatttgggtc aactttaaag tctttggcct gaccgccctt accttaatct tcacattgtt
     3181 aagcggtatc tatatctacc gccacatgcc gcaggaagat aaatcctaac cagactgcca
     3241 gtccttcggg gctggcgctg tatctcctct gttctcctca ggaaaatcat agtagcatcg
     3301 cgcctgtgat tttcctttta agtcggtttt accatgtcta caacacataa cgtccctcag
     3361 ggcgatcttg ttttacgtac tttagccatg cccgccgata ccaatgccaa tggtgacatc
     3421 tttggtggtt ggttaatgtc acaaatggat attggcggcg ctattctggc aaaagaaatt
     3481 gcccacggtc gcgtagtgac tgtgcgggtt gaaggaatga ctttcttacg gccggttgcg
     3541 gtcggcgatg tggtgtgctg ctatgcacgc tgtgtccaga aagggacgac atcggtcagc
     3601 attaatattg aagtgtgggt gaaaaaagta gcgtctgaac caatcgggca acgctataaa
     3661 gcgacagaag cattatttaa gtatgtcgcg gttgatcctg aaggaaaacc tcgcgcctta
     3721 cctgttgagt aatagtcaaa agcctccggt cggaggcttt tgactttcta cttactgaat
     3781 ttcggtggtg ccgttaattt taaacaggat attcaccaca atcccactgc ctggcttacc
     3841 cggctcataa cgccatctgc gcatcgcatt tttcacctca cgctcaaaca tgttcgcagg
     3901 cttggctgag aggatttgta cgttatccac gcgaccatcc ggcgtgacgt caaatttaac
     3961 tttaacctgc ccttcaatgc gcaatgcctg tgctcgtgcc ggatactgcg gctgattacg
     4021 gcttaatgcg cgtggtcctg aagccacact ggtaaccggc ttgctggttg cagccgttgc
     4081 cgtacttgat gtggggcgcg cgggtgccgt attttcaaac ggtgatgccg gacgcgactc
     4141 tacgggtttg acatcgcgtt ttggctgctc ctgtaccttt ttcaccggct tcggttttgg
     4201 cttaggtttc ggcttcggct tttcaatgac caccggtgct tcttttggcg gttcggggat
     4261 cggctcaggt tccggttctg gctctaccac cggctccggt ggcggctgaa cggcttgtgg
     4321 cggttcgaga tcagcaggcg caaccatcgt gacagaaatt ggctgcgcag gcgcaggtag
     4381 ttcaataacc tgatgtaccg aggtatagag cagacccgcc acaacagcac catgaatgca
     4441 gaccgaaagt aacgtcggcc aggggaagcg gcgaggtaaa tcaagggtca ttgtattgta
     4501 gctattcggc attgtagcat tagccataac gtttataagt tcatctttaa atgataatca
     4561 attgcatggg agtttatcac cgtgagaacc gtaataacat acttgagatt ctcatcagcc
     4621 atacaaggcg ctgaaggtgc agattcaacc agacgacaaa acgacctgtt caagcagtgg
     4681 ctgaagaaga atggcgatgc tcaaatagta gcgtctttca gtgatgaagg gttgagtggt
     4741 tacaaaggta aacaccttac tggtcagttt ggtgacatgt tagcccgtat tgaggctggg
     4801 gagtttccag aaggtacaat tctgctagtt gagagtatcg accgtatagg ccgattagag
     4861 catcttgaaa cagaagcctt gatgaaccgc atcttaggca acgggataga gattcacaca
     4921 ttgcaagatg gccttatcta cacaaaggat gctttagccg atgaccttgg cataagcatc
     4981 atccagcgtg tcaaagccta catagcgcat cagaaatcta agcagaagtc tttccgtgtt
     5041 tctcagaaat gggggcaacg tgcaaaactt gcccttgctg gtgaacaacg tcttacaaag
     5101 atggttcctg gatggattga ccctgagact tttaaactca atgaacacgc tgagactgtg
     5161 agattgatat tcaaactgct gctagatggt gaaagtctgc ataacattgc acgtcacctt
     5221 caaagcaacg gtataaagtc gtttagtcgc cgtaaagatg ctaatgggtt ctctgttcac
     5281 tctgtacgca ccattctaag gtcagagaca acaataggca cgttaccagc atcacaacgt
     5341 aatgaccgcc ccgctatacc gaactactac gaaggtgttg tagatatacc aacgttcaat
     5401 aaagctcaag agattctcga caagaatcgt aaaggccgta cacctgcaag tgacaaccca
     5461 ctaacgatta acatcttcaa aggtctgttt aggtgtcagt gtggggctag tgtccatcct
     5521 accggaacaa agaataagta tgctggggtc tacaggtgca ataaccactt agacggtcgc
     5581 tgtgatgttc caccgttgaa gcgtaaaccg tttgaccgat ggatgattga taattttctg
     5641 gggatgattg acgtggggaa tgatggagaa tcagagagaa agattgcagc gttacagcat
     5701 gaggttgaaa ttgtcacagc cagaatcaag aaagctaccg ccctacttct tgagatggat
     5761 gatattgatg aactaaaaat tcagcttaag gaactgaacc agaagcgcac agaactacag
     5821 accacgattg ataacatgag gcgtaaggct tcactcactg ataaggaact accccagctt
     5881 aaagatattg accttatgac taaagcgggt cgtgtcgagt gtcagttgat tttgtccaag
     5941 catctaaaag ggcttacatt gggtaaggat tcggtaactg taacgctaca gaacgacact
     6001 gaaatcacca tacctacaaa cccgttaccg ctaaatgatg ggtcaccaat ctttgaaatt
     6061 gctgataaag agctactgga tattgacgct tatcaactgt aattttcagg ggctatgaag
     6121 ccccttttta tgtctcaata agtgcccttc ttgttagggt tcacagagta ctttcgcaga
     6181 gcattacccg gcttaagcac ctttgaagct tgctcattag cggcatctga gaaggtgctc
     6241 tcaccatttt taacgctaac gctcttagag cacgttacac gggcttttag cgcttcatct
     6301 gataactcac ttgccgaata gccaaaaagc tctttacagg tcagcatagg gcttccaagg
     6361 ctcgttgtag tgtttccggt gttcttttta ctggtagcac cgccatcatt cgtggtagcg
     6421 gtctttccag tagcagccga aacatcagtc tgacctacga ttgaacgaaa ctctacacct
     6481 tggaaggtca tctgaatacg aagaccgtta gcaatggtat gctctttact cgctttaaac
     6541 tgagtaatga tactatcagt gatagctatt ccatctctac aaacaatagt gatgatttgc
     6601 ttctggtctc gccattgttc aaccgtagaa ataaaggttt ctacaagttg cccctgtctg
     6661 gttaatagaa ctgaacccgt cgaatcaacc actacaacac cgctaataga gatggtctta
     6721 ggtgctctct
//
LOCUS       DAZPDU010000214         6553 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZPDU010000214 DAZPDU010000000
VERSION     DAZPDU010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6553
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.216"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            142..1317
                     /gene="mdtL"
                     /locus_tag="ACHURS_003971"
     CDS             142..1317
                     /gene="mdtL"
                     /locus_tag="ACHURS_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFR1239584.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            1292..2251
                     /gene="yidZ"
                     /locus_tag="ACHURS_003972"
     CDS             1292..2251
                     /gene="yidZ"
                     /locus_tag="ACHURS_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFR1239585.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            2408..3157
                     /locus_tag="ACHURS_003973"
     CDS             2408..3157
                     /locus_tag="ACHURS_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFR1239586.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MGLQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            3179..3745
                     /gene="yieF"
                     /locus_tag="ACHURS_003974"
                     /gene_synonym="chrR"
     CDS             3179..3745
                     /gene="yieF"
                     /locus_tag="ACHURS_003974"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFR1239587.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDRQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(3799..5136)
                     /gene="adeP"
                     /locus_tag="ACHURS_003975"
     CDS             complement(3799..5136)
                     /gene="adeP"
                     /locus_tag="ACHURS_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFR1239588.1"
                     /translation="MSHQHTTQTSGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            5302..5967
                     /gene="yieH"
                     /locus_tag="ACHURS_003976"
     CDS             5302..5967
                     /gene="yieH"
                     /locus_tag="ACHURS_003976"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFR1239589.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTESEHVYRAEVARLFDSELEAIEGAEALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            6034..6507
                     /locus_tag="ACHURS_003977"
     CDS             6034..6507
                     /locus_tag="ACHURS_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HFR1239590.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT         1608 a         1655 c         1659 g         1631 t
ORIGIN      
        1 atttggctaa atccttcaag aagccagcca ttcgctggct tcttgcctct caggaaatca
       61 cttatgtcca aatggcaact cgcctgatcc tccttcacca cgtatgcttt gcgtcacctt
      121 actatcagga cgctttagcc catgtcccgc tttttgattt gtagttttgc cctggtttta
      181 ctttatcccg ccgggattga tatgtacctc gttggtttac cgcgcatcgc cgccgatctc
      241 aatgccagcg aagcgcagtt gcatattgcg ttctccgtat atctggcggg gatggcagct
      301 gcgatgttat ttgccggtaa agtggctgat cgttcaggga gaaagccggt cgccataccc
      361 ggcgcggcgc tatttattat tgcctcggtg ttctgttcac tggctgaaac cagcacgtta
      421 tttcttgcag gccgatttct acaggggttg ggcgcaggct gttgttacgt agtggcgttc
      481 gccattttgc gcgacacgct ggatgatcga cgtcgggcta aagtgctgtc attactcaac
      541 ggtattacct gcatcattcc ggtgttagcg ccagtgctcg gacatctgat tatgcttaaa
      601 ttcccgtggc agagtctgtt ctgggcgatg gcaatgatgg gcatcgcggt actgatgttg
      661 tctttgttta ttttaaaaga aacgcgccca gcgtcccccg ctgcttcgga caaaccacga
      721 gaaaatagcg agtcgctgct taatcggttt ttcctcagcc gtgttgttat caccaccctc
      781 agcgtttcgg tgatcctcac tttcgtcaat acatcgccgg tattgctgat ggaaatcatg
      841 ggttttgagc gcggagaata cgccaccatt atggcgttga ccgctggcgt cagcatgacc
      901 gtttcattct ccacgccatt tgcgctggga atttttaagc cacgtacgtt gatgatcacc
      961 tcgcaggtgt tattccttgc ggcggggatc acccttgccg tttcaccttc ccatgcggtt
     1021 tctctgtttg gtatcacgct gatttgcgcc ggtttctcgg taggttttgg cgtagcgatg
     1081 agtcaggcgt taggaccatt ttcattacgc gcgggcgtag ccagctcgac cttaggtatt
     1141 gcgcaggttt gcggttcgtc actgtggatt tggctggcag cggtggttgg tattggcgca
     1201 tggaatatgc tgatcgggat tctgattgcc tgtagtatag tgagcctgtt gctgattatg
     1261 ttcgtcgcgc ctggacgccc cgttgccgct catgaagaaa tccatcacca cgcttgatct
     1321 caatctgctg ctctgtctgc aactgctgat gcaggagcgc agcgtgacca aagcggcgaa
     1381 gcggatgaac gtgacccctt cagcggtgag taagtcgctg gcaaagttaa gagcgtggtt
     1441 tgacgacccg ctctttgtga actcaccgct gggcctgtcg cccacaccgc taatggtcag
     1501 catggagcaa aatctggcgg aatggatgca aatgagcaat ctgctgctgg ataaaccgca
     1561 ccaccagaca ccgcgcggcc tgaagtttga gttggcggcg gaatcgccgc tgatgatgat
     1621 catgcttaat gcgctgtcga aacggatcta ccaacgttac ccgcaggcga ccatcaaact
     1681 acgtaactgg gattacgatt ccttagatgc cattactcgt ggtgaagtgg atattggttt
     1741 ttccggtcgc gaaagccatc ctcgctcgcg ggagctgtta agctcgctac cgttagccat
     1801 tgattatgaa gtgctgttta gtgatgtacc ctgcgtctgg ttacgcaaag atcatccggc
     1861 actgcatgaa acgtggaatc tggacacctt tttacgttat ccgcatatca gcatttgctg
     1921 ggaacagagc gacacctggg cgctggacaa tgtgttacag gagctggggc gcgaacgcac
     1981 gattgctatg agtctgccgg aattcgagca gtcactgttt atggcagcgc aacccgacaa
     2041 tctgctactg gcgaccgcgc cgcgctactg tcagtactac aatcaactcc atcaactgcc
     2101 gttggttgct cttcctctcc cgtttgacga aagccagcaa aaaaagctgg aagttccttt
     2161 taccctgctg tggcataaac ggaacagcca taatccgaag atcgtctggt tacgggaaac
     2221 cattaaaaac ctttacgcgt cgatggcata accgaatcgt atgaaacgcg acccaatttc
     2281 acaataaaat gtaaaaaagt tgtaacaagc ttgtctgaat cgaactttta gccgctttag
     2341 tctgtccatc attccagtaa atgattactc ttgtattcat aatggaccat taagcatgga
     2401 gcgaaaaatg gcgactcact ttgcccgagg gattttaacg gaaggacatc tgatttctgt
     2461 tcgtctcccc tcccagtgtc atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg
     2521 ttttctggcg tccagaggtt tactcgcaga actgatgttc atgctgtatg gcattggcga
     2581 attgccggaa atcgtcaccc tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc
     2641 ttcgttttct atttcctatg ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga
     2701 atgtggcctc gatatgggac tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc
     2761 cgataaccac accttttcca gcaatgaatc gctatggatc agtaaacaaa acgatcctaa
     2821 cgaagcgcgg gcgcagctca tcacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt
     2881 tttgaatgac gatccgcgcg atctgcagct gctgcccatt gccggacgcc tgaaatgtgc
     2941 tcatgtaaat catgtagaag cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc
     3001 ggtcacgccc gcgattgaaa agctcagtgt ctgggagtta gatggcaaac acagctggaa
     3061 aagcctgccg gatattcaca gccgcgccaa caatcctacc agccggatga tgcgttttgc
     3121 ccagctctct accgtgaagt ctttttcgcc aaattgatag acaaccacag gagtcattat
     3181 gtctgaaaaa ttgcaggtgg ttacgttact ggggagcctg cgcaaaggct catttaatgg
     3241 catggttgca cgtaccctgc cgaaaattgc tccggcgagc atggaagtca atgcgttacc
     3301 ttccattgcc gacattcccc tgtatgacgc tgacgtacag caggaagaag gttttccagc
     3361 aacggttgaa gctctggcgg aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc
     3421 ggaatataac tactcggtac cgggtgggct gaaaaatgcc atcgactggc tttcccgcct
     3481 gccggatcaa ccgctggccg gtaaaccggt gttgattcag accagctcaa tgggcgtgat
     3541 tggcggcgcg cgctgtcagt atcacctgcg ccagattctg gttttcctcg atgcaatggt
     3601 gatgaacaag ccggaattta tgggcggcgt gattcagaac aaagttgatc ggcaaaccgg
     3661 agaagtgatt gatcagggta cgctggacca cctgaccggg caattgaccg catttggtga
     3721 gtttattcag cgagttaaga tctaaataaa aaacccgcca gtaatcatgc atggcgggtt
     3781 tttaatgcgc tatcgatttt aatgagcgtc gataaataca atcttcagga taaacagcag
     3841 cgcaacgatg attacgcacg ggctaaggtc acgcagacgc ccggtaccaa tcttcatcac
     3901 gcagtaggag ataaagccca gcgcaatacc ttcggtaatc gaaaagctga acggcatcat
     3961 cacggcggta ataaacgccg gaacagattc agtaagatcc tgccagttca cgcgagcaag
     4021 acttgaggtc atcaacacgc caacgtagat caacgcgcca gctgcagcgt agcctggcac
     4081 cattcccgcc agcggcgaca gaaagataac cagcaggaac agcagaccaa caaccactgc
     4141 cgtcagaccg gtacgaccgc caaccgatac gccggaagag gactcaatat aagccgtaac
     4201 ggaagaagta ccgataaacg aaccggtcac ggaagagata ctgtcgacat acagcgcctg
     4261 cttcatgcgc gggaatttcc ccttctcatc cgccagacct gctttatcgg tcacgccaat
     4321 cagcgtaccg gaggagtcaa acaagttgac caacatgaaa gagaaaatca cccctgccag
     4381 cccgaggtta aacgacccgg ctaaatctac atgacccaca actgtcatta cgctcggcgg
     4441 cgcagaaacg atgccattgt agtgcacatc acccagcatc cagcccagca gcgtcgtcac
     4501 cacgatagaa accagcaccg ctgcgtgaat gttgcgcgag gccagaatgg cgatgatgaa
     4561 gaaaccgagg atccccagca gcacgctgtg agaagtcaga ttaccgatgc tcaccagcgt
     4621 ttccgggtta gcgacaatca cacctgcgtt tttcagcccc atcatgccaa tgaacagacc
     4681 gataccgctg gtaataccca cacgcagact taccggaatg ttagcaatca tccagtaacg
     4741 aacgcggaaa atggtcaaaa gaagcagtcc gacagcgccc cagaagattg cgcccatacc
     4801 gatctgccac ggcagcccca ttgcctggac aaccacaaaa gcgaaaaagg cgttaagccc
     4861 catcgcagga gccagcgcca ccggaaggtt tgcaaacaat cccatcataa tgctaccgaa
     4921 agcagcaatc agacaggttg ttacgaagac ggcactggtg tccatgccag caacgcccag
     4981 aatttgtggg ttaacaaata cgatgtagac catcgtcagg aaggtggtga acccggcaat
     5041 cacttcagtt cgtgccgtag taccatgttc gcgcagttta aaaactcgtt ccagcatccc
     5101 ctgaccagaa gtctgggtgg tatgttgatg actcattatc tatttccgaa caaggaggga
     5161 aaatccgtcg ctatcgtata ccaaaatgcg acaataggcg cgtttgtgag agactttttt
     5221 tattggattt acttatacgg caacgattgc gttgcgcaaa tcggctctac gaaaacgtta
     5281 aactgattaa aaaggaaagg catgtcccgg atagaagcgg tatttttcga ctgcgacggt
     5341 acgctggtcg acagtgaagt catttgctcc cgcgcatatg taacgatgtt tcaggaattt
     5401 ggtattacgc tcgatcctga agaggtattc aaacgtttca aaggtgtaaa actgtacgaa
     5461 attatcgata ttgtttccct tgaacatggt gttacgttag cgaaaacaga atctgaacac
     5521 gtttaccgtg cagaagtcgc tcggctgttc gattcagaac tggaagccat cgaaggggct
     5581 gaagcgctcc tgtcagcgat cactgcgcca atgtgtgtgg tatctaacgg cccaaataac
     5641 aaaatgcagc attctatggg caagctgaat atgttgcact acttcccgga taaactgttc
     5701 agcggctacg atattcagcg ctggaagcca gacccggcgt taatgttcca tgcggcaaaa
     5761 gcgatgaatg taaatgtaga aaactgcatt ctggttgatg actcagttgc cggtgcgcaa
     5821 tctggtatcg acgcaggtat ggaagtgttc tacttctgcg ccgacccgca caataagccg
     5881 atcgttcacc cgaaagtcac cacctttacc catctttcgc agttaccgga actgtggaaa
     5941 gcgcgtggtt gggatattac ggcatagttc ttcacactcc cttcacttac cccgcttaaa
     6001 ttggcgctca aaggtaagta aagggagttt gatatgtctg tttcacgtcg ggtaatacat
     6061 cacggacttt attttgcggt tttaggaccg ttaattggtg ttctgtttct tgtcctctac
     6121 atattcttcg caaaagaacc gctggttctt ttggtaataa tacaggtact tccattgttt
     6181 ctcttattgt cgattactac gggagctatt cctgcattgt taaccggcgt gatggtcgcc
     6241 tgtctgccgg aaaagatcgg gtcacagaaa aattatcgtt gtctggctgg tggcataggt
     6301 ggcgtcgtta tcaccgagat ctattgtgca gttattgtac atattaaggg catggcttcc
     6361 tcggagttgt ttgaaaacat tctttctggt gacagtctcg ttgtccgcat cattcctgca
     6421 ttgctggcag gtgtggtgat gagcagaatc attacccgtc tgcccggatt ggatatttca
     6481 tgtcctgaaa cagactcttt aagttaagcg ggatacttta tctttgggct actcaaaagc
     6541 agacaggatg tta
//
LOCUS       DAZPDU010000215         6500 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZPDU010000215 DAZPDU010000000
VERSION     DAZPDU010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6500
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.217"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            171..1769
                     /gene="rtcR"
                     /locus_tag="ACHURS_003978"
                     /pseudo
     CDS             171..1769
                     /gene="rtcR"
                     /locus_tag="ACHURS_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
     gene            complement(1751..2509)
                     /locus_tag="ACHURS_003979"
     CDS             complement(1751..2509)
                     /locus_tag="ACHURS_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFR1239591.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(2526..3356)
                     /gene="glpG"
                     /locus_tag="ACHURS_003980"
     CDS             complement(2526..3356)
                     /gene="glpG"
                     /locus_tag="ACHURS_003980"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFR1239592.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPALKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(3401..3727)
                     /gene="glpE"
                     /locus_tag="ACHURS_003981"
     CDS             complement(3401..3727)
                     /gene="glpE"
                     /locus_tag="ACHURS_003981"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFR1239593.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            3917..5422
                     /gene="glpD"
                     /locus_tag="ACHURS_003982"
     CDS             3917..5422
                     /gene="glpD"
                     /locus_tag="ACHURS_003982"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFR1239594.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDRM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(5474..6079)
                     /locus_tag="ACHURS_003983"
     CDS             complement(5474..6079)
                     /locus_tag="ACHURS_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239595.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(6082..>6500)
                     /locus_tag="ACHURS_003984"
     CDS             complement(6082..>6500)
                     /locus_tag="ACHURS_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239596.1"
                     /translation="QRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINSV
                     PGFWSTVSLACLLNRYCEDYIRDNQDDIDNAITTQINAWARNNHPLLKERARQAIENP
                     LKQPEPMTEYQVWEDDWIIDECLCAMYDASSESPGK"
BASE COUNT         1639 a         1695 c         1663 g         1503 t
ORIGIN      
        1 ttgtttgctg tcgtgataat tgcaaccgtc gtgccagaaa attaaataag cagattaatt
       61 ttttaattca ttgtttttaa ataggttaaa tctttacgtc cgtaaacgga gattttccac
      121 aaaaccaatt caccgataat gaaatatcgt cttttataag gatatctaag atgcgtaaaa
      181 cagtggcttt tggctttgtc ggtaccgtgc tggattatgc cggacgcggc agtcagcgct
      241 ggtcgaaatg gcgtccgacg ctttgtttat gccagcaaga atcgttagtc atcgatcggc
      301 tggaattgtt gcacgacact cgctcgcgct cgctattcga aacgcttaaa cgcgatatcg
      361 ccagcgtttc gccagaaaca gaagtcgtgg gcgttgagat tgaactgcat aacccgtggg
      421 atttcgaaga ggtttacgcc tgcctgcatg atttcgcccg tggttacgag tttcagccag
      481 aaaaagaaga ctatttaatt cacatcacca ccggcaccca cgtcgcgcag atttgctggt
      541 ttctgctggc ggaagcgcgt tacctgcccg cccggctgat acaatcttca cctccgcgca
      601 aaaaagaaca gccgcgcggc gcaggtgaag tgacgattat cgatctcgat ttaagccgtt
      661 ataacgccat cgccagccgc tttgccgagg aacgccagca aacgcttgat tttcttaagt
      721 ccggcattgc cacgcgtaac ccccacttca accgcatgat tgagcagatc gaaaaagtgg
      781 cgatcaaatc ccgcgcgccg attctgctta acggtccaac cggcgcgggc aagtcatttc
      841 tggcgcgacg catcttcgag ttaaaacagg cgcggcatca gtttagcggc gcgtttgtgg
      901 aagtgaactg cgccaccctg cgcggcgata ccgccatgtc gacgctgttt ggtcatgtaa
      961 aaggcgcgtt taccggggcg cggtaatctc gtgaaggttt attacgcagc gccaacggcg
     1021 gaatgttgtt tcttgatgag attggcgaac tgggcgcaga cgaacaggca atgctgctaa
     1081 aagccattga agagaaaacc ttttacccgt ttggcagcga tcgccaggtg agcagtgatt
     1141 ttcagcttat cgccggaacg gtgcgcgatt tgcgccagct ggttgccgaa ggcaaatttc
     1201 gcgaagatct gtacgcgcgg atcaatctct ggaccttcac cctgccgggt ctacgccagc
     1261 gccaggaaga tattgaaccg aaccttgatt atgaagtgga gcgccacgcc acactcactg
     1321 gcgacagcgt gcgttttaac accgaagcgc ggcgcgcctg gttggctttt gcgacctctc
     1381 cccaggcaac atggcgcggt aactttcgcg aactttctgc cagcgtcacg cggatggcca
     1441 cctttgccac tagcggacgc atcactctgg acgtggttga agatgagata aaccgtctgc
     1501 gctataactg gcaggagagc cgcccctccg cgcttacggc gttgctgggc gcagaggcag
     1561 aaaacatcaa tctcttcgac cgtatgcaac tggaacacgt tatcgctatc tgccgccagg
     1621 caaagtcgct ttctgcagcc ggacgacagc tttttgatgt ttcgcgccag ggcaaagcca
     1681 gcgtcaatga cgcggatcgg ctacgcaaat acctggcgcg ttttggtctg acgtgggaag
     1741 ccgtgcagga tcagcacagc tccagttgaa tatgatggtc cgtcagcacc tgcattacgc
     1801 ttactggcgg cggggcgtcg gtgtagacgg catctaccat gctgatgctg cccatattga
     1861 ccatcgcgtt acggccaaat ttcgagtgat cgacaaccag cataacgtgg cgcgagttct
     1921 caataatggc gcgcttggtg cgaacttcgt ggtaatcgaa ctccagcagc gagccgtcgc
     1981 tatcgatgcc gcttatcccc agaatgccga aatcaaggcg gaactgggag ataaaatcga
     2041 gcgtcgcttc gccaatgatc ccgccatcgc ggctgcgtaa ttcgccaccg gcgagaataa
     2101 tgcggaaatc ttcttttacc atcaacgtgt tagcaacatt gagattgttg gtgacaatac
     2161 gcaaattgct gtgattaagc agcgcgtgcg ccaccgcttc cggcgtggtg ccgatatcga
     2221 taaacagtgt cgagccgttg gggatttgct ccgccacttt gcgggcaatc ctctcttttt
     2281 cttcggtctg ggtggctttg cgatcgtgcc acggcgtgtt aaccgaactg gaaggcagcg
     2341 ccgcaccgcc atggtgacgc aggatcaggt tttgctccgc cagctcattg aggtcgcggc
     2401 gaatagtctg cgggctgacg gagaaatgct ctaccagctc ttcagtactg acataaccct
     2461 gctgtttaac cagttcgata ataccgttgt gacgttgtgt ttgtttcatt tataaatccc
     2521 tggaattatt ttcgttttcg cgcattgagc gaatcaacaa aagccatcgc taaacccacg
     2581 gctaacccgg cgatgtgtgc tccgttcgcc atcgacatcc caaacaaatc aaaccatccg
     2641 gcgacaatcc agatcagcgc aaagataatt aacccacgtt gcaggtaaat gccactttgc
     2701 ggatcgcgtt caccacgtag ccagacgtag cccatcagcg catacaccac gccagaaagc
     2761 ccgccaaacc acggcccgct gaatttttgc tgcacatagc cgcttaacag ggcgctaatg
     2821 agagtaataa caattagctt accgctaccg aggcgttttt ccaccgcacc gccgagatac
     2881 caccaccaga gcaggttaaa gaggatatgc atcagcgaga agtgcattaa cgcgtgggtg
     2941 aagtaacgcc agaactcaaa tttcagtgct ggatcgaatg gccaggccag ccataacatc
     3001 acttcctgat cgccgagaat ttgcatggca ataaacacca ccacgcaggc gatcatcatc
     3061 acccaggtca ccggacctgc gcgttcacgc aaggcggcaa agaaaggata acggcgataa
     3121 tgcaggccac tgccggtatg gcctgcctgc cagctggccg ccagataacg cggatctgcc
     3181 gggttttcga gaaaacgcgc cagctccgcc cgtacgcgct cggcctggga ctcatccgcc
     3241 agccagacat cgctttggtt atgttgttga atcgtgagga taacaccctg cgtcgccatg
     3301 taatcaacaa acgcctgcgc cacgcggggg ttagcaaaag aggtaatcat caacatcgtt
     3361 gctgtcgctt attccacaca aaaggggaca gtataaagcg ttacgcgccg tacgccacct
     3421 ctgcgggaaa ctgacgttgc caggcttcaa agccgccgtc aatgctatag accacatcgt
     3481 agccctgttg cagcagatac tgcgccgcgc ctttgctgct attgccgtga taacacatca
     3541 ccatcaccgg agtgtcaaag tcgttatcac gcataaacgc gcccagcgtg tcgttggtta
     3601 aatggaaagc ctgcaccgca tgccccatag cgaaactctg tggatcgcga atatcgacca
     3661 gcaccgcctc tttttcctgc aacttctggt gcgcgtcggc aacgttaata cattcgaact
     3721 gatccatgcg tctctctttc tttacaaaca agtgggcaaa tttaccgcac agtttacgtc
     3781 gaagcggcag ataaacgcca taatgttata catatcactc taaaatgttt tttcaatgtt
     3841 acctaaagcg cgattctttg ctaatatgtt cgataacgaa catttatgag ctttaacgaa
     3901 agtgaatgag ggcagcatgg aaaccaaaga tctgattgtg ataggtggcg gcatcaatgg
     3961 tgctggtatc gcggcagacg ccgctggacg cggtttatcc gtgctgatgc tggaggcgca
     4021 ggatctcgct tgcgcgacct cttccgccag ttcaaaactc attcacggtg gcctgcgcta
     4081 ccttgagcac tatgaattcc gcctggtcag cgaggcgctg gctgaacgtg aagtgctgct
     4141 gaaaatggct ccgcatatcg ccttcccgat gcgttttcgc ctgccacatc gtccgcatct
     4201 gcgcccggcg tggatgattc gcattggtct gtttatgtac gatcatctgg gtaaacgcac
     4261 cagcctgccg ggatcaactg gtttgcgttt tggcgcaaat tcagtgttaa aaccggaaat
     4321 taagcgcgga ttcgaatatt ctgactgttg ggtagacgac gcccgtctgg tactcgccaa
     4381 cgcccagatg gtggtgcgta aaggcggcga agtgcttact cggactcgcg ccacctctgc
     4441 tcgccgcgaa aacggcctgt ggattgtgga agcggaagat atcgataccg gcaaaaaata
     4501 tagctggcaa gcgcgcggct tggttaacgc caccggcccg tgggtgaaac agttcttcga
     4561 cgacaggatg catctgcctt cgccttatgg cattcgcctg atcaaaggca gccatattgt
     4621 ggtgccgcgc gtgcataccc aaaagcaggc ctacattctg caaaacgaag ataaacgcat
     4681 tgtgttcgtg atcccgtgga tggacgagtt ttccatcatc ggcaccaccg atgtggaata
     4741 caaaggcgat ccgaaagcgg tgaagattga agagagtgaa atcaattacc tgctgaaagt
     4801 gtataacacg cactttaaaa agcagttaag ccgtgacgat atcgtctgga cctactccgg
     4861 tgtgcgtccg ctgtgtgatg atgagtccga ctcgccgcag gctattaccc gtgattacac
     4921 ccttgatatc catgatgaaa acggcaaagc accgctgctg tcggtattcg gcggtaagtt
     4981 gaccacctac cgcaaactgg cggaacatgc gctggaaaaa ctgacgccgt attatcaggg
     5041 cattggcccg gcatggacga aagagagcgt gctgccgggt ggtgccattg aaggcgaccg
     5101 cgacgattac gcagctcgtc tgcgccgtcg ctatccattc ctgactgaat cgctggcgcg
     5161 tcattacgca cgcacttacg gcagcaacag cgagctgctg ctcggcaatg cgggggcgat
     5221 aagcgacctc ggggaggatt tcggtcatga gttctacgaa gcggaactga aatacctggt
     5281 ggatcacgaa tgggtccgcc gcaccgacga tgccctttgg cgtcgcacaa aacaaggcat
     5341 gtggctgaat gcggatcaac aatctcgtgt gagtcagtgg ctggtggaat atacgcagca
     5401 gaagttgtcg ctggcgtcgt aaggatgatg cccggttgaa gtgcaaccgg gctatttgga
     5461 ttccttagac tattcattgt aaataattga ataacggccc ctgagctaaa atcgccaaac
     5521 ttaagcacgt tacccaatcg ccccacttct tcctctggtt tctatttttt ctgcaccaat
     5581 ggcaataata tcccaatgca aaaccaagct gtgcactaat cgggccgaca aaatcagtcc
     5641 aaggctgcgg ccaaaaaggg gaaaaattca ggggaggcca gacaaacgtc agaaagaaat
     5701 ttcggatatt ttccagcaac gctgatttct ctggagaaat ataaagaatg aaattcgaga
     5761 gaattaacca gaaatagacc ggcgaaaaga ccagccagca atgcagtctg ttttttaatt
     5821 caggaggcgt tctgcgggag aattgccaac cgatgccagc cattacactg aaaaaaagtg
     5881 catattcaac cattaactca agttgtaatt cctgaagaga aatacgcaca accaggagcg
     5941 gaaaggagaa tattatttct gccagaaaag ttaacgtgaa tataagcacg gctaagacaa
     6001 taaccgtcgg ccataccaat aatggcacga taacaaaacc gagaattaca gggggaatat
     6061 aaagccggaa tttaatcatt tttattttcc tggtgattcg gatgatgcgt catacatcgc
     6121 gcataaacac tcatcaatta tccagtcatc ttcccagacc tgatattctg tcattggttc
     6181 gggctgtttt agcgggttct caatcgcttg ccgcgctctt tctttcagta aaggatgatt
     6241 atttctagcc caggcattga tttgggtggt aatggcgtta tcgatatcgt cctggttatc
     6301 tctgatataa tcctcacaat atctgttcaa taaacaggca agagaaacgg tcgaccaaaa
     6361 gccgggaact gaattaatgg catccagcaa tggcgcaaga gcgatatcgg cacggaaaaa
     6421 catttccagc atatactgta attcgacagc ttcatcgccg gatacccaat cagtcatata
     6481 ctggcaggca aatcgctgta
//
LOCUS       DAZPDU010000216         6472 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZPDU010000216 DAZPDU010000000
VERSION     DAZPDU010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6472
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.218"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            137..571
                     /gene="slyA"
                     /locus_tag="ACHURS_003985"
     CDS             137..571
                     /gene="slyA"
                     /locus_tag="ACHURS_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFR1239597.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(618..1085)
                     /gene="slyB"
                     /locus_tag="ACHURS_003986"
     CDS             complement(618..1085)
                     /gene="slyB"
                     /locus_tag="ACHURS_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFR1239598.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            1359..2468
                     /gene="anmK"
                     /locus_tag="ACHURS_003987"
     CDS             1359..2468
                     /gene="anmK"
                     /locus_tag="ACHURS_003987"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFR1239599.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            2566..2895
                     /gene="mliC"
                     /locus_tag="ACHURS_003988"
     CDS             2566..2895
                     /gene="mliC"
                     /locus_tag="ACHURS_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFR1239600.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            2954..3610
                     /gene="pdxH"
                     /locus_tag="ACHURS_003989"
     CDS             2954..3610
                     /gene="pdxH"
                     /locus_tag="ACHURS_003989"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFR1239601.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            3739..5013
                     /gene="tyrS"
                     /locus_tag="ACHURS_003990"
     CDS             3739..5013
                     /gene="tyrS"
                     /locus_tag="ACHURS_003990"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFR1239602.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            5072..5935
                     /gene="pdxY"
                     /locus_tag="ACHURS_003991"
     CDS             5072..5935
                     /gene="pdxY"
                     /locus_tag="ACHURS_003991"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFR1239603.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEVLEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(5979..>6472)
                     /gene="gstA"
                     /locus_tag="ACHURS_003992"
     CDS             complement(5979..>6472)
                     /gene="gstA"
                     /locus_tag="ACHURS_003992"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFR1239604.1"
                     /translation="NGDDYFAVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQL
                     LAPVNSISRYKTIEWLNYIATELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEAL
                     KDEHWICGQRFTIADAYLFTVLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAE
                     GLK"
BASE COUNT         1639 a         1601 c         1670 g         1562 t
ORIGIN      
        1 aatttacgcg caatttctga aaaatatgtc tgacggtaac caaatgcagc aatacatttg
       61 ttttagcaat acaattgctg cacactattc taaaagccgc ataatatctt agcaagctaa
      121 ttataaggag atgaaattgg aatcgccact aggttctgat ctggcacggt tggtgcgcat
      181 atggcgtgct ctgatagacc atcgcctgaa accgctggag ttaacacaaa cccattgggt
      241 tacgttacac aatatccatc agttacctcc agaccagtcg caaattcaac tggcaaaagc
      301 gattggcatc gagcagccat cactggtccg tactctggac caactggaag aaaaagggtt
      361 aatttcgcgt caaacttgtg ccagcgatcg tcgggctaaa cgtattaaac tgacggaaaa
      421 ggcagagcca ctgatcagcg aaatggaagc tgttattaac aaaacccgcg cggaaatatt
      481 acatggcatc tccgcagagg aactggagca actgattacg ctcatcgcaa aacttgagca
      541 taatatcatt gagttacagg ccaaagggtg aaatgaaggg ggcgtgtggt cagtaacctg
      601 accacacgca aacttattta gcgcggagaa acggtcacct gactgccatt gctggccagt
      661 acgacacgtt ggcccggaga gaaacgagtg ttgccttgtt tctgtaccac catgatggta
      721 ttaccatcgt ctttacgaat ttccagctcg acaccctgcg ttttgttcat tgcactctgt
      781 acgccctgac cagctacgcc acctgcaaca gcgcctgctg cagtagccag agaacgcccg
      841 gttccgccac caacagtatt ccccaggaaa ccaccaagaa cagcaccgcc aattgcaccg
      901 ataacgttgg aatcatcacc gccctgaatc tgtaccggac gtacgttaac gatggtgcca
      961 tagctgacat tctgtacttg tttcgcttca gaagcggtat aaacatcccc tgacagggtg
     1021 tcgttattaa cacaaccgac aagagacaga cctaccattg aaacaaccaa tacgcgttta
     1081 atcattgaaa aatctcctgt tcaccatgaa acgccacgca agcatccctc atggtcaaag
     1141 tatatggcat atgtgaatcc atggttacat gttctaccaa atatgagcaa atcataatga
     1201 aagtaggatc aaccaattat aaacaaaaca ttcatgatga ttattgattt gaagataatg
     1261 ttttactacc aacggtaaaa atcggtcatt ccgcatggta tccacgaaaa gtttatgttt
     1321 gagtggtgcg caaacgtcag attgaaaaag gatgaactat gaaatcgggc cgctttattg
     1381 gcgttatgtc aggcaccagc cttgatggtg ttgatgttgt gttggcgaca atagatgaac
     1441 accgggtcgc acagctggca agcttgagct ggccgatccc ggtgtctctg aaacaggctg
     1501 tactggatat ttgccagggc cagcagctta cactttcgca gtttggacag cttgatactc
     1561 aactcgggcg actttttgct gatgcggtca atgccttgct taaggaacaa aacctgcagg
     1621 cgagagatat agttgcgatc ggctgtcacg gtcaaaccgt ctggcatgaa ccgacgggcg
     1681 tggcaccaca cactttacag attggcgata acaatcaaat tgtggcacgc accggaatta
     1741 cggttgtcgg tgattttcgc cgtcgcgata ttgccttggg aggacaaggc gcaccgctgg
     1801 tacctgcgtt ccatcatgcc ttgctggctc acccaaccga gcgacgaatg gtgctcaata
     1861 ttggcggcat cgccaatctg tcactgctca ttcctgggca gccggttggc ggctacgata
     1921 ccggtcctgg taacatgctg atggatgcct ggatctggcg tcaggccggt aaaccttacg
     1981 ataaagatgc cgagtgggca cgggcgggta aagttattct cccactgctg caaaatatgc
     2041 tcagcgaccc atatttctcg caacctgcac cgaaaagcac cggacgcgaa tactttaact
     2101 acggctggct ggagcgccat ttacgccatt ttccgggtgt tgatccccgt gatgttcagg
     2161 ccacactggc agaactcacc gccgtgacca tttctgaaca agttttgttg agcggtggct
     2221 gcgaacgatt gatggtatgt ggtggaggta gtcgtaaccc gctactcatg gcgcgtctgg
     2281 cggcattact gccaggcaca gaagtcacca ccaccgatgc cgttggcatt agtggcgatg
     2341 acatggaagc attggcattc gcctggcttg cctggcggac gctggcggga ttaccaggaa
     2401 atctgccttc cgtcactggc gcaagccagg agacggtact gggggctatt ttccctgcta
     2461 acccgtgata tcagagttaa ctgaattttt cttaccgcgt ctgccgttac acttgaaacg
     2521 ttagggaggg cgtattgccc tccagaccag gaaagtcttc gggaaatgtc tatgaaaaaa
     2581 ctgttactga tctgtttgcc ggtgcttctc accggctgta gcgcgttcaa tcaactggtt
     2641 gagcacatgc aaaccgctac gctggaatac cagtgtgatg aaaaaccgtt gacggtcaaa
     2701 ctgaataatc cgcgccagga agtcagtttt gtttacgata atcaactact gcatctcaaa
     2761 cagggcattt cagcgtctgg tgcacgttac actgacggaa tctatgtttt ctggtcgaaa
     2821 ggcgatgaag cgactgtcta taaacgcgac cgcatcgtat tgaataactg tcagttacaa
     2881 aatccacagc gttgagattt ttccaggggc ggcgcacaat agcgtcaccc actgacaatc
     2941 cgtaaagaaa accatgtctg ataacgacga attgcagcaa atcgcgcatc tgcgccgtga
     3001 atacaccaaa ggcgggttac gccgtcgcga tcttcccgcc gatccattaa ccctttttga
     3061 acgttggctc tctcaggcgt gtgaagccaa actggcagac cctaccgcga tggtggtcgc
     3121 taccgtggat gaacatgctc agccttatca acgcatcgtt ttactcaaac attacgacga
     3181 aaaaggcatg gtgttttaca ccaacctcgg cagccgtaaa gcacatcaaa ttgaaaataa
     3241 tccgcgcgtt agcctgctgt tcccgtggca tacccttgag cgccaggtga tggtgatcgg
     3301 taaagcggaa cgactttcga ctctcgaagt gatgaaatat tttcatagcc gcccgcgtga
     3361 tagccagatt ggtgcatggg tttcgaagca gtccagtcgc atttctgccc gcggtatcct
     3421 tgaaagtaaa ttcctggagc tgaagcagaa gtttcaacag ggcgaagtgc cattgccgag
     3481 cttttggggc ggttttcgcg tcagccttga acagattgag ttctggcagg gcggtgagca
     3541 tcgcctgcat gaccgctttt tgtaccagcg tgaaaatgat gcgtggaaga ttgatcgtct
     3601 tgcaccctga aaagatgcaa aaatcttgct ttaatcgctg gtactcctga ttctggcact
     3661 ttattctatg tctctttcgc atctggcgaa aagtcgtgta ccggcaaagg tgcagtcgtt
     3721 atatacatgg agattttgat ggcaagcagt aacttgatta aacaattgca agagcggggg
     3781 ctggtagccc aggtgacgga cgaggaagcg ttagcagagc gactggcgca aggcccgatc
     3841 gcgctctatt gcggcttcga tcctaccgct gacagcttgc atttggggca tcttgttcca
     3901 ttgttatgcc tgaaacgctt ccagcaggcg ggccacaagc cggttgcgct ggtaggcggc
     3961 gcgacgggtc tgattggcga cccgagcttc aaagctgccg agcgtaagct gaacaccgaa
     4021 gaaactgttc aggagtgggt ggacaaaatc cgcaagcagg ttgccccgtt cctcgatttc
     4081 gactgtggag aaaactctgc tatcgcggcg aacaactatg actggttcgg caatatgaat
     4141 gtgctgacct tcctgcgcga tattggcaaa cacttctccg ttaaccagat gatcaacaaa
     4201 gaagcggtta agcagcgtct caaccgtgaa gatcagggga tttcgttcac tgagttttcc
     4261 tacaacctgt tgcagggtta tgacttcgcc tgtctgaaca aacagtacgg tgtggtgctg
     4321 caaattggtg gttctgacca gtggggtaac atcacttctg gtatcgacct gacccgtcgt
     4381 ctgcatcaga atcaggtgtt tggcctgacc gttccgctga tcaccaaagc agacggcacc
     4441 aaatttggta aaactgaagg cggcgcagtc tggttggatc cgaagaaaac cagcccgtac
     4501 aaattctacc agttctggat caacactgcg gatgccgacg tttaccgctt cctgaagttc
     4561 ttcaccttta tgagcattga agagatcaac gccctggaag aagaagataa aaacagcggt
     4621 aaagcaccgc gcgcccagta tgtactggcg gagcaggtga ctcgtctggt tcacggtgaa
     4681 gaaggtttgc aggcggcaaa acgtattacc gaatgcctgt tcagcggttc cttgagtgcg
     4741 ctgagtgaag cggacttcga acagctggcg caggacggcg taccgatggt tgagatggaa
     4801 aagggcgcag acctgatgca ggcgctggtc gattctgaac tgcaaccttc ccgtggtcag
     4861 gcgcgtaaaa ctatcgcctc caatgccatc accattaacg gtgaaaaaca gtccgatcct
     4921 gaatacttct ttaaagaaga agatcgtctg tttggtcgtt ttaccttact gcgtcgcggc
     4981 aaaaagaatt actgtctgat ttgctggaaa taatgcatta agtggaaagg gggagtgaga
     5041 aatcactccc cctggttttt atacagggaa catgatgaaa aatattctcg ctatccagtc
     5101 tcacgttgtt tatggtcatg cgggtaacag tgcggcagag tttccgatgc gccgcctggg
     5161 cgcgaacgtc tggccgctga acaccgttca attttctaat cacacccaat acggcaaatg
     5221 gactggctgc gtgatgccgc ccagccattt aaccgaaatt gtgcaaggca ttgccgctat
     5281 tgataaatta cacacctgtg atgccgtgtt aagtggctat ctgggatcgg cggagcaggg
     5341 tgaacatatc ctcggtatcg tccgtcaggt gaaagcagcg aatccgcagg cgaaatattt
     5401 ttgcgatccg gtaatggggc atccggaaaa aggctgtatc gttgcaccgg gtgtcgcaga
     5461 gtttcatgtg cggcacggtt tgcctgccag cgatatcatt gcgccgaatc tggttgagct
     5521 ggaaatactc tgtgagcatg cggtaaataa cgtcgaagaa gcggttctgg cagcgcgcga
     5581 actcattgcg caagggccac aaattgtgtt ggttaaacac ctggcgcgag ctggctacag
     5641 ccgtgaccgt tttgaaatgc tgctggtcac cgccgatgaa gcctggcata tcagccgtcc
     5701 actggtggat tttggtatgc gccagccggt aggtgttggt gatgtgacga gcggtttact
     5761 gctggtgaaa ctgcttcagg gggcaacgct gcaggaggtc ctggaacatg tgaccgctgc
     5821 agtctacgaa atcatggtga ccaccaaagc aatgcaggaa tatgagctgc aagtggtggc
     5881 tgctcaggat cgtattgcca aaccagaaca ttacttcagc gcaacaaagc tctgaaatat
     5941 cacatttcgg cccacaacga cctgtgggcc gatggctatt actttaagcc ttccgctgac
     6001 agcgcgtctt gtacttccgg acgttcagcc atacgttgca taaatgccgc aatgtgctct
     6061 aacccttcca gattcagttt caccgcgtat gcccagcgca gaaccgtaaa cagataggca
     6121 tcagcaattg taaatctttg cccgcagatc cagtgctcat ccttcagtgc ctcgttcaca
     6181 tattgcagct tcttatccag ctgcgcgcga actgtcggtt tgtactcttc cggtgtatct
     6241 gggcgaaaca gaggtgtgaa acctttatgc agctcggtgg cgatgtaatt cagccattcg
     6301 atggttttat agcgggaaat actgtttacc ggtgccagca actggcggtc ggggacgctg
     6361 tcggcaagat actgcataat cgctacgcct tccgtcagca aagtaccgtc atccagcagc
     6421 aatgctggca cctgcccctt agggttaacg gcaaagtaat cgtcaccgtt tt
//
LOCUS       DAZPDU010000217         6431 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZPDU010000217 DAZPDU010000000
VERSION     DAZPDU010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6431
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.219"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..331
                     /locus_tag="ACHURS_003993"
     CDS             <1..331
                     /locus_tag="ACHURS_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFR1239605.1"
                     /translation="IEAWRTEQEAKPFTFEWNGRIWNAGPNSLGRLYPVVMAAKSDIV
                     RDVMTWGDADNQQVKLSMPELEELAAAMAQAQVERNDEIYRRQRELKEELNSLKDLNS
                     VRNFIVE"
     gene            complement(532..1389)
                     /gene="sitD"
                     /locus_tag="ACHURS_003994"
     CDS             complement(532..1389)
                     /gene="sitD"
                     /locus_tag="ACHURS_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HFR1239606.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISAKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1386..2243)
                     /gene="sitC"
                     /locus_tag="ACHURS_003995"
     CDS             complement(1386..2243)
                     /gene="sitC"
                     /locus_tag="ACHURS_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HFR1239607.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(2240..3067)
                     /gene="sitB"
                     /locus_tag="ACHURS_003996"
     CDS             complement(2240..3067)
                     /gene="sitB"
                     /locus_tag="ACHURS_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HFR1239608.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNDSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(3067..3981)
                     /gene="sitA"
                     /locus_tag="ACHURS_003997"
     CDS             complement(3067..3981)
                     /gene="sitA"
                     /locus_tag="ACHURS_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HFR1239609.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            complement(4567..5001)
                     /gene="uspF"
                     /locus_tag="ACHURS_003998"
     CDS             complement(4567..5001)
                     /gene="uspF"
                     /locus_tag="ACHURS_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFR1239610.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            complement(5142..6275)
                     /gene="ompN"
                     /locus_tag="ACHURS_003999"
     CDS             complement(5142..6275)
                     /gene="ompN"
                     /locus_tag="ACHURS_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HFR1239611.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAADGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
BASE COUNT         1750 a         1516 c         1552 g         1613 t
ORIGIN      
        1 aattgaagca tggcgcacag aacaggaggc gaagccgttc acgtttgaat ggaacggtcg
       61 tatctggaat gctggcccca actcactggg ccgcctttat ccggtggtaa tggctgcgaa
      121 atccgacatt gtacgggacg tgatgacgtg gggtgatgcc gataatcagc aggtgaaact
      181 gtcgatgccg gaactggaag aactggcggc agcaatggct caggcacagg ttgagcgcaa
      241 tgacgagatt tatcgccgtc agcgggagct gaaggaagag ctgaatagtc tgaaggattt
      301 gaattcggtt aggaatttta tcgtggaata acagaagctg cggcacgtcg tatgcaggaa
      361 cgtgccgcgg ttggctggta aactttcgat agtgcgagta ttgaatgatt tctaggcgtt
      421 atcgatttta cgtatttttt gcatgataga attcgtacct cctcccaacg accttccatg
      481 gctttccgga cttctgtagg ctggcggacg atgctgtcct cggatgttgt gctaattggg
      541 tgataacaga tcctgtgtat gggcgtttcc ctttgtgcgt gcgttgatgc tggtaacggt
      601 aaacgtcatg atgaacacga ccgcgaataa gacgacaatc gtcggagctg gtgcgctgtc
      661 gataaaaaag gacagccaga caccagtcat tgacactatt accgatatgc cagtcgccag
      721 taggagcgca atatgaaagc gttgtgtcag gagtactgca atggcgccag gcgcaatgag
      781 cagagaaata gagagaatga tacctactgc tttcagcgtc gccacaatgg tcagggaaac
      841 catgcacagc agcccataat gcagccatct cgtatgcaaa ccgcttacct gcgcctgttg
      901 ataatcaaag ctgaacagca aaaaatcccg ccattttgcg ctaataacaa gtgtaaccag
      961 cccggcaata atcaccgtct ggattatatc gccgatggtt atcccgagca tgtcgccgaa
     1021 aagaatatgg tcaagatgta cgtctggttt gattgcaata tacaagatca gacctgcggc
     1081 aaacatgcca gaaaaaacaa tccccataac tgtatcttgc ttgattcggc tgttgtcttt
     1141 caggtatccc gtcgccaccg cgcagaatac tccggcgacg aatgcccctg tagccagagg
     1201 taaccccaga atccaggtaa gcacgatgcc aggaaaaacc gcatgactca tcgcgtcacc
     1261 catcagtgcc caacctttca ggaccagaaa gaccgacagc agcgcacagg gtatggaaac
     1321 taccagtgaa atcagcagtg cgtgactcat aaaggtgaac tgcagtggct ccagtaataa
     1381 tgccatcatg agcgatcctc cagcgcttta tgcgcacgcc gacgatttgc caaaagtcca
     1441 tgggtgggcg caaatacaaa tgctaaaaga aacagcagtg tctgcgccac cacgatgatt
     1501 ccacctgtcg cgccgtccag aaagtaactg acccacgcgc cgaggaaact ggttacactg
     1561 ccaatagtta cagcaattat gagcaggcgg ggaaaacgat cggttaacaa ccatgctgtt
     1621 gcaccgggag tcaccaccag acagatcacc agaaaggcac caaccgtttg cagcgccgca
     1681 acggtcgaaa ccgacagcag ggtgaagaag aggattttta atctgcccgg atgtaatcca
     1741 atagcacggg cgtgattctc gtcgaaaaag gtcaccataa gatctttcca tttgaaaaaa
     1801 agcacgatga tcgagagaat gccaatgata gtgagttgca ggatatccgc cggatcgatt
     1861 gccagaatat tgcccaggac gatggtctga atgttcacgg atgtcgggtt caatgacacc
     1921 ataaacagcc ccaggccaaa aaaggacgag aagatcaggc cgatgatggc atcttctttt
     1981 aagcgggtgc gttggttaag aaacagcatg ctgcccgctg ccagtccgcc ggaaaagaag
     2041 gctcccagag aaaacggaag ccccagcata tatgcccccg caacacccgg gacaatagag
     2101 tgcgacagcg catcgccaat cagcgaccag ccttttagca tcagatagca tgacaggaaa
     2161 gcgcagagac cgccgaccat tgccgagacc cacatcgcat tgagcatata ttcatagctg
     2221 aagggttcca gtagcacgtt catcttgctt ccctctgcac cgctgacggt cgatgtgcca
     2281 caaaagggcg ttcatcgtca gtaataatgc tttcttccga gtcgttaagt gtgacatggc
     2341 gcagtacgcc gctaaaagcc agctccaggt tctcggctgt aaaagtggtg tctgtcggcc
     2401 cgcttgccag gacggtgcct ttgaccatga ccgtataatc gcaaaatgtc gtgactgagc
     2461 cgagattgtg cgttgagaca agcatcgttt tgccttccgc gcgtaactcc ctcaacaggc
     2521 tgatgatttt cgcttcggtt ttcacatcaa cgccggtaaa aggttcatcc agcaggatca
     2581 catcaccctg ctgtgcaatc gctctcgcca gaaagacgcg tttcttttgt ccaccggaaa
     2641 gctcgccgat ttgacgatgg cgaaaatcca ccatatcgac acgttccagc gcatcggtga
     2701 caatctgacg atccctcttt ttggctatac gcaacatacc catatgccca tagcggccca
     2761 tcatcaccac atcttccacc agtacaggaa atgaccagtc aacctcttct gactgaggga
     2821 cgtaggcaac caggtttttc tgtagcgcct gtcgcgtggg aatacccaga acagatatct
     2881 ttccgctggt cagacgcaca aatcccataa tcgctttaaa caacgtcgat tttccggaac
     2941 cgtttacccc aaccagagcg gcaatagatc cacttggtac tgtgaaggat gcatcgcgca
     3001 gtgctgtgtg cccgttacgc caggtgacag taacatcatt aacgacaatg cctgcagatt
     3061 gcatcattat ttctcccttt tcccggcttt aattccctgt accagcgtac tggtagtaac
     3121 cttaagaagg tcgatatacg taggtaccgg gccgttttct gtgctcaggg aatcgacata
     3181 gagcacaccg ccgtagtgtg cgccggtttc acgcgcaacc tgacgcgctg gtttatcgga
     3241 aatcgtactc tcgctaaaga ctgccgggat atgatttttc ttaactatat caacaacctt
     3301 acgtacctgc tgcggtgttc cttgttgatc ggcattaatc ggccacagat aaagctcttt
     3361 tagccccaaa tcgcgtgcga gataagaaaa agccccttca ctggtgacca tccatcgctg
     3421 attctcaggg agttccgtaa tctgcttacg caggggggca agggtttggg taatcttggc
     3481 tttataagta tcggcattac gttggtaggt ttgtgcattt gccggatcgt attttatcaa
     3541 cgcatcacga atattatcga cgtaaatcag agcattatct ggcgacatcc atgcatgggg
     3601 attaggtttg ccctcatagg gtccttcggt gatccctact ggcgtcacac ccgaagagac
     3661 aattacttct ggaaccccat tgagatgctg gtaaaagcgt tggaaccaca attccagatt
     3721 cataccattg gcgagaatca gttgtgcccc ctgcgcacgt ttaatatcgc caggggtagg
     3781 ctgatactca tgaatttctg caccaggctt ggttatggat gagacttctg cagcatctcc
     3841 agccacgttt ttggccatat ccgcgatgat ggtaaatgtt gtaatgacct tgaatttttc
     3901 agttgcactt gcctgaaatg cgatcgagca ggtgagggcg aggcacccca agagcatggt
     3961 tacttttttt attgagtgca taatttagta cctatagtga gcaaaattag aacaaacata
     4021 gcaacggcta tgttttatag cataacccat acaaaatgaa aatgattatc attaacagct
     4081 ttgaggccta aaacagggat taggttgttt cctgctggcg acatgatatt gaggaaaaaa
     4141 gtagagcagc tattaggctt tgaaatgact ttatgaggaa aattgttgat ggcaaaaatg
     4201 gggcaaaacg ctgtagaagg ggcaaaaaag gggcaaaaaa agagtgggtt atcgtagctt
     4261 attgttgtcg ctgataatgt ttaacatatt gaaaaataaa taaaatactt atgggtcaga
     4321 tagttgtgat ttttgccctt acttgttcag gttgtattgt tctttcttac taatttcttg
     4381 attttgcgac atttaaaagc gactcaattc gttatatggc atcagaagag tatgcgtcat
     4441 gccggaacgc ccagcataag aaatctgata taaaactgtg gcgtgtatgg tacggattag
     4501 aggggaaaat gtcagcacat ttgcgaaatg aatcaaaaag cccgcagcaa tgtgcgggcg
     4561 ttagtgtcag cgcacaacca gcacggagca ctctgcgtga cgcactacag ctgcggcgtt
     4621 ggaaccgagc agataagtgg tgatatccgg tcgatgggaa gcaatgatga tcatatgagc
     4681 ggggatcttc ttcgccaatt ccagaatgcg gtctttgggc gagccttcct caacatggac
     4741 atgcactctg tcggttggca gtttaaattt tttaatgatc tcttccagtt gcgatttggc
     4801 ttccgctttc aggtcatcca ttgccggtaa ttctgcggaa tacgctaaac ccagagaggc
     4861 atagtaaggc agtgaaggta ttaccgtcag gaaatgaacc tctgcatcgt caatctttgc
     4921 ctctgcctca acgtggctaa tcacgcgttg agttaattct gaatcggaaa tatcgatagg
     4981 gacaagaatc gttctgttca taaaacctcc tgttttagta tccgcataaa gtgtaacgcc
     5041 agatgacact ttttgtgtaa tgacggagtt cacattttta atttagatca aaggaggaag
     5101 aataagcaga aaaagcccgc cataacagcg ggcaggagga tttagaactg ataaaccaga
     5161 cctaaagcga caatatcatc ggtagagatg ccgttggcag cgtagaaact gtcatcttca
     5221 tccaacaggt tgattttata gtcaacataa gtggacatgt ttttattgaa atagtaagtc
     5281 gcgccaacat cggcgtattt aacaagatct ttatcatcaa cacctgccgg gttgtctgca
     5341 ccatcggcag cgtgcaggtc acggccttta gacatcagga aagagactgc cggacgcaga
     5401 ccaaaatcaa actggtactg tgcagtgact tcaaagttct gggttttgtt tgcaacagca
     5461 taatcgctgt cgccaaacgg ggtcatatta cgcgtttctg aatacatggt tgccaggtaa
     5521 atattgttag catcgtattt tagcccagca gtccacgcgt ctgctttatc accacccgcc
     5581 gcagtatggt taacctggtc attggtgcgg tcagatgagg tgtatgccgc accagcgcta
     5641 aagcccatgc ctaaatcata tgttgtggaa agaccccagc cgtcaccatt ttcatggcga
     5701 acatcacgtc cgttgttggt gccttcctga ccattactgg caccttcgtt gttaccttga
     5761 tactgcaccg cgaagttcag accatttacc agaccgaaga aatcagtatt acgataagtc
     5821 gcgacgccgt tggcacgacc agtcataaag ttgtctgcat tggtataaga gtcaccgcca
     5881 aattcaggca gcatatcggt ccagccttcg atgtcgtaca ttacgccata attacgtccg
     5941 taatcgaaag aaccgtaatc tgcaaatttc agcccggcaa atgccagacg ggtccatgac
     6001 tggttttttg aagattcagt gttgtttgcc tgaatattgt attcccattg accgtagcca
     6061 gtgagttgat cgttaatttg ggtttcgcct ttaaaaccca gacgcgcata gctctgatcg
     6121 ccatctttcg ctgaattatc agaaaaataa tgcaggccat caactttgcc atacagatct
     6181 aatttgttgc cgtctttatt ataaacttcg gctgcatgtg cagcacctgc ggcgagcagg
     6241 gcaggaatta aaagtgccag tactttgctt ttcattgaat aaatccttta gttattttat
     6301 ttgcctttta tccccattcc ggggagtgaa aacatcctaa aggagaagtt cagttagccg
     6361 ataaatatca tttgttacgc cttaagtaaa accttaatca aataattctt ctgaaatgat
     6421 aataagtctg g
//
LOCUS       DAZPDU010000218         6391 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZPDU010000218 DAZPDU010000000
VERSION     DAZPDU010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6391
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.220"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(135..509)
                     /locus_tag="ACHURS_004000"
     CDS             complement(135..509)
                     /locus_tag="ACHURS_004000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239612.1"
                     /translation="MRKKENKAKKEKFRQFFPENYDIGISLNEENQLRLFSKRNDSQD
                     ESLCKYEFSNKIKVQYIFLPYSSEIQVITDEFPLTEAGCQECTQAFKHPISMELIEEI
                     KEAKCSVTGLVIYSKPILKLIS"
     gene            complement(832..2766)
                     /locus_tag="ACHURS_004001"
     CDS             complement(832..2766)
                     /locus_tag="ACHURS_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR1239613.1"
                     /translation="MLRPICYERVSSIQQVDMGKGLDDQRSSLTAYLDKNADKFTDER
                     IFITDAGLSAFKNANISPESQLGIFLQDVGNRKYGVGDALVVLSLDRLSRRSSWAEST
                     IQYIVNSGIEIHDISTNLVLRVDDPMSKIHMELIQHRSHNESLMKSVRAKLAWDNKIT
                     RAVQNGEVISNRMPNWLRNVDNKYQVIPEQAELIVKCFMWYREGLSTGEIVKRIGRKW
                     QMVTVSRLIRDRRLLGEHERYNGEVVPNVYPKIIDEELFLTANRMMDRVMLEKKKPAE
                     DLLLEPEVVREIFKLYETGLGSGAIVKRLPKGWSTVNVLRVLRDKKVVTMKIIDNLTF
                     ERANEKLSRNGIANRIRKDITIAQDDYITNLFPKMLKCGHCGGNIAIHYNHVRTKYVI
                     CRNREEKKICDAKSIQYMRIEKNILQLVKNVDFNKLMMEGKSDSDSLLASLREELSTL
                     QKEAETYQVKINERKAAGKKASLPLMTGLTEVQDRIEEIESEILNSKNIKEIPVFDFD
                     INKVLDPLNVELRAKLRKEVRLVLKSVKYWVFEKRIFVQLEYFNDVLSHTLVIDNKRG
                     GGEVLHEIVISETSGERVYTVNEEGKTVFIASVTSGTDMWSLAISRTKILDDVGLYLN
                     LLKREKFEIIVNEDMIDWMD"
     gene            2846..4780
                     /gene="yeaG"
                     /locus_tag="ACHURS_004002"
     CDS             2846..4780
                     /gene="yeaG"
                     /locus_tag="ACHURS_004002"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFR1239614.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMKGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            4893..6176
                     /locus_tag="ACHURS_004003"
     CDS             4893..6176
                     /locus_tag="ACHURS_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFR1239615.1"
                     /translation="MTGFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKQPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
BASE COUNT         1788 a         1493 c         1333 g         1777 t
ORIGIN      
        1 gaaagatgta aagtacaaag cctactatct cgtaaagtaa tttagtaaac cattccattt
       61 tgaagtaccc ccattcacat tcaactttat agtatttagt gagggagtac ttaaaacgta
      121 acaatttgga tgtgttagct aataagtttt agtataggtt tcgaataaat gacaagtcca
      181 gtaactgaac attttgcttc ttttatctct tcaattagtt ccattgatat tggatgtttg
      241 aaagcttgag tacactcctg acagcctgcc tccgttagcg gaaactcatc tgttataact
      301 tgaatttcag aagaatacgg caaaaaaatg tactgaactt taattttgtt ggaaaattcg
      361 tacttgcata agctctcatc ttgagaatca ttccgctttg aaaaaagcct taattgattc
      421 tcttcattca atgagatccc aatatcataa ttctcaggga aaaattgacg gaatttttct
      481 ttttttgctt tattttcttt cttacgcatt ttggcaatcc tctctttaga attcatctca
      541 tggtttcatc gctcctattc gttagtgaat gacgctatgg tactggttta acgttgaaaa
      601 gtcaaaaaag ttaattttac ccttgactat tctaacacac gctgatataa atgatttgct
      661 gtattttgca cggaaatctt aaacccatcg ggtgcgtagt tatatccaaa attcataatc
      721 cctcttttat tgctctatct acaatgccgt tagatttcga catcgtagat atattgttca
      781 aatattaact cgcataattc attctacaag accataaatt ctcattagct tttaatccat
      841 ccaatcaatc atatcttcat taacaatgat ctcaaatttt tcacgcttga gaagattaag
      901 atacaatcca acatcatcaa gtattttggt acggctaatg gcaagcgacc acatatcagt
      961 accagatgta acggaggcaa tgaaaacggt tttgccctct tcattgacag tgtagacacg
     1021 ctcgccgctt gtttcagaga ttacgatctc atgtagtact tcacccccgc cccgcttgtt
     1081 atcaatcaca agcgtatggg ataggacatc attgaagtat tccaactgta caaatatacg
     1141 cttttcaaaa acccagtact taacagattt cagtactaat cttacttctt tccgtaactt
     1201 tgcacgtaac tcgacattca atggatcaag tactttatta atatcgaaat caaatacagg
     1261 gatttctttg atgttctttg aatttaatat ctcagattct atttcttcaa ttctatcctg
     1321 tacctcagtt aaacccgtca ttaatggtaa cgaggctttc ttacctgctg ccttacgttc
     1381 attaatttta acctgatagg tttctgcctc tttctgtaaa gtacttaact cttctcgcaa
     1441 tgatgcaagc aatgaatctg aatccgactt accttccatc atcaatttat tgaagtctac
     1501 atttttgaca agctgtagaa tgttcttttc aattctcatg tactggatag atttagcatc
     1561 gcagatcttc ttttcttccc tgttacgaca aataacatac ttagttcgta catgattata
     1621 gtgaattgca atattgcccc cacagtgacc gcatttcagc atcttcggga agaggttagt
     1681 aatgtaatca tcctgagcaa tggtaatgtc cttacggata cgattagcta taccattccg
     1741 cgataacttc tcattagccc gttcaaaggt gaggttatcg ataatcttca tggtaactac
     1801 tttcttatca cgtagtaccc tcagtacatt gacggtactc caccctttcg gcaagcgttt
     1861 tacaatcgcc cctgaaccaa ggcctgtctc ataaagttta aaaatctctc ggacaacttc
     1921 tggttcaagt aatagatcct ctgctggctt cttcttctcc aacataacgc gatccatcat
     1981 tctattcgct gtcaggaaca actcttcgtc gatgattttc gggtaaacgt ttggtactac
     2041 ctcaccgttg taacgttcat gctcgcccaa caaacggcga tcacgtatca gcctggacac
     2101 agtgaccatc tgccactttc taccgatacg tttaacaatc tcgccagtac tcaacccttc
     2161 cctataccac atgaaacatt taacaatcag ttctgcctgt tccggtataa cttggtactt
     2221 gttatctaca tttctcaacc aattaggcat acggttggag attacttccc cgttctgtac
     2281 tgcacgtgtg attttgttat cccatgccaa ttttgcacgt accgatttca tcaatgactc
     2341 attgtgtgaa cggtgctgta tcagttccat gtgaattttt gacataggat catcaacccg
     2401 taacacaagg tttgtagaga tatcgtgaat ctcaatccca ctattcacga tgtactggat
     2461 agtactttcg gcccagcttg atcgacgcga tagacgatcc agcgataaaa ccacaagtgc
     2521 gtcacctacg ccgtacttgc ggttgcccac atcttgcaaa aatatcccaa gctgagactc
     2581 tggagagata ttggcatttt tgaatgcgga aagccccgca tcagtaatga aaatcctttc
     2641 gtcagtgaac ttatcagcat ttttatctaa gtatgcagtc aatgatgatc gttggtcgtc
     2701 caaaccttta cccatatcaa cttgctgaat gcttgatact cgctcataac aaataggacg
     2761 gagcatattt tttatcccgt tgaagaatta cgctaaaagt gctgattcac aaatgttccc
     2821 agagtataca aaaggacggc ataccatgaa tatattcgat cactatcgcc agcgatatga
     2881 agctgccaag gacgaagagt tcacactgca ggagtttctt accacttgtc ggcaagatcg
     2941 cagtgcttat gccaacgcgg ctgagcggct attgatggct atcggtgagc ctgtcatggt
     3001 cgatacagcc caggaaccca gactttctcg actcttttct aaccgggtca ttgcacgtta
     3061 tccggcattt gaagagtttt acggcatgga agacgcgatt gaacagattg tctcttatct
     3121 gaaacacgcg gctcaggggc tggaagagaa gaaacaaatc ctgtatctgc tggggcctgt
     3181 gggtgggggt aaatcatcgc ttgctgagcg actgaaatca ttaatgcagc tcgtaccgat
     3241 ttatgtattg agcgccaacg gtgagcgtag cccggtcaac gatcatccgt tctgtctttt
     3301 caatccgcag gaagatgcgc agattctgga aaaagagtat ggcattcctc gccgctatct
     3361 cggcaccatc atgtcgccgt gggcggcaaa acgcctgcat gaatttggtg gcgatatcac
     3421 taagttccgg gtagtgaagg tctggccgtc aattctgcaa caaattgcta tcgccaaaac
     3481 agaacccggt gatgagaaca accaggacat ctccgcgctg gtagggaaag tcgatattcg
     3541 taaactcgaa cactacgcgc agaatgaccc ggacgcctac ggctattccg gtgcgctgtg
     3601 ccgcgccaat caggggatca tggaatttgt cgagatgttt aaagcaccga ttaaagtgct
     3661 gcatccgctg ttaaccgcta ctcaggaagg taactacaac gggacggaag gtatctccgc
     3721 cctgccgttc aacgggatta ttctcgccca ctcgaacgaa tccgaatggg tcactttccg
     3781 taataacaaa aacaacgaag ccttcctcga ccgtgtttac atcgtgaagg tgccgtattg
     3841 cttacgcatt tccgaagaga tcaaaatcta cgagaaattg cttaatcaca gtgaattgac
     3901 tcacgcccca tgcgcccctg gcacgctgga aacactgtca cgtttttcca ttctttcgcg
     3961 cctgaaagag ccagaaaact ccagcattta ttcaaagatg cgggtttatg atggcgaaag
     4021 tctgaaagac accgatccca aagccaagtc gtatcaggaa tatcgtgact acgccggtgt
     4081 cgatgaaggg atgaaaggtc tgtcgacgcg ttttgcgttt aagatcctct cccgcgtgtt
     4141 caacttcgat catgtagaag tggcggcaaa cccggtccat ctgttctacg tcctagaaca
     4201 gcagatcgag cgcgagcagt tcccgcaaga gcaggcagaa cgctatctgg agttcctgaa
     4261 aggttatctg atcccgaaat atgccgagtt tatcggcaaa gagatccaga cggcctacct
     4321 tgaatcctat tccgaatatg ggcaaaacat tttcgaccgt tatgttacct acgcggattt
     4381 ctggattcag gatcaggagt atcgcgatcc ggataccggg cagctgtttg accgcgagtc
     4441 tcttaacgcc gagctggaga aaatcgagaa accggcgggg atcagtaatc caaaagattt
     4501 ccgcaacgag attgttaact tcgtactgcg cgccagagcg aataacagcg gacgcaatcc
     4561 gaactggacc agctatgaaa aactgcgcac ggttatcgag aagaaaatgt tctccaatac
     4621 cgaggagctg ttgccggtta tctcgtttaa cgccaaaacg tcaaccgacg agcagaagaa
     4681 acacgacgac tttgtcgacc gtatgatgga aaaaggctac acccgtaaac aggtgcgttt
     4741 actgtgcgaa tggtatttgc gcgtacgtaa atcgtcttaa caaccctggc ccggtacaca
     4801 tgctaccggg cctataacga cagcgaaccg tgggccagag aaatggcaac acaggcgtag
     4861 catacagttg gcaaatgtag tacggggggc atatgaccgg gtttattgac cggcgtctga
     4921 acggcaaaaa caaaagcatg gtgaatcgcc agcgtttttt acgccgttat aaagcgcaaa
     4981 ttaaacagtc gatctccgag gccattaata agcgttcggt gactgacgtc gacagcggcg
     5041 aatccgtatc cattcccacg gaagatatta gcgaaccgat gtttcatcag gggcgtggcg
     5101 gcctgcgcca ccgcgtacat ccgggcaatg accatttcgt ccagaacgac cgaattgaac
     5161 gtccccaggg tggcggcgga ggttccggca gtggtcaggg ccaggccagc caggatggtg
     5221 aaggtcagga tgaatttgtc tttcagattt cgaaagatga gtatcttgat ctgctctttg
     5281 aagatttggc cttaccgaat ctgaaacaaa accaacaacg ccagctgacc gaatataaaa
     5341 cgcatcgggc gggttatacc gctaacggcg ttccagccaa tatcagcgtt gtgcgttcct
     5401 tgcagaactc actggcgcga cgcacagcca tgacggcggg caagcgacgg gaacttcatg
     5461 cgctggaaga gaatttggcc atcatcagca acagtgaacc tgcgcaactg ctggaagagg
     5521 aacgtctgcg caaagaaatt gcagaattac gtgccaaaat tgaacgcgtc ccttttattg
     5581 acaccttcga tttacgttac aagaactacg agaagcagcc cgatccctcc agccaggcag
     5641 tgatgttttg cctgatggac gtttccggtt caatggatca atccactaaa gatatggcta
     5701 agcgttttta tattctgctg tatctgttcc tcagcagaac gtataagaac gtggaagtcg
     5761 tatacatccg ccatcatacc caggcgaaag aagtcgatga acatgagttt ttctactcgc
     5821 aggaaacagg cggcaccatt gtttccagcg ccctgaaact gatggatgag gtagtgaaag
     5881 agcgttataa cccggcacag tggaatattt acgctgcaca agcatcggac ggcgataact
     5941 gggccgatga ctctccgctt tgccatgaaa tcctggcgaa aaaattatta cctgttgttc
     6001 gttattacag ctatatcgaa attacccgtc gtgcacatca gacattgtgg cgagaatatg
     6061 agcatctgca atctactttc gacaactttg cgatgcagca catccgcgac caggatgata
     6121 tttatccggt gttccgtgaa ctgtttcata aacaaaatgc aacagctaaa gactaaaact
     6181 atcagccagg tcattatcgc ctggctgatt tttagcttac tgtaaattat ctcatttatt
     6241 acatacatta gcttacaatc gctttaaata tgacagcata acctttacat aatttagttc
     6301 cagaaaacaa tcattcggaa aaatgattca gtcaacacgt atttccatgg ggttattctt
     6361 taaatatttt ttatcgttaa cgaaaattga t
//
LOCUS       DAZPDU010000219         6330 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZPDU010000219 DAZPDU010000000
VERSION     DAZPDU010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6330
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.221"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..357
                     /gene="mcbR"
                     /locus_tag="ACHURS_004004"
     CDS             <1..357
                     /gene="mcbR"
                     /locus_tag="ACHURS_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFR1239616.1"
                     /translation="LTPQDLTELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSN
                     MPILCEMIEQLWVRMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLA
                     EIMQQNIAILYQQYNR"
     gene            complement(393..2495)
                     /gene="pqqU"
                     /locus_tag="ACHURS_004005"
     CDS             complement(393..2495)
                     /gene="pqqU"
                     /locus_tag="ACHURS_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFR1239617.1"
                     /translation="MKIFSVRQTVLPALLALSPVVFAADEQTMIVSAAPQVVSELDIP
                     AAVSVVDGEEMRLATPRINLSESLTDVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            2737..3798
                     /locus_tag="ACHURS_004006"
     CDS             2737..3798
                     /locus_tag="ACHURS_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFR1239618.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGVTINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(3913..5412)
                     /gene="ansP"
                     /locus_tag="ACHURS_004007"
     CDS             complement(3913..5412)
                     /gene="ansP"
                     /locus_tag="ACHURS_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFR1239619.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGMPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            5679..6296
                     /locus_tag="ACHURS_004008"
     CDS             5679..6296
                     /locus_tag="ACHURS_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFR1239620.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGAGHEWFQDNATNISAIADAVCQLPKLQEVLKRNEII"
BASE COUNT         1705 a         1596 c         1513 g         1516 t
ORIGIN      
        1 ctcaccccgc aagacctgac ggaactccag gaattgctcg agaaattaca gcaggcgcaa
       61 gaaaagggcg acatggaaca aatcattaat gtaaacaggc tatttcgctt agcgatttat
      121 catcgctcaa atatgcccat cctgtgtgag atgattgagc aactgtgggt caggatgggg
      181 cctggtttac attatctcta tgaagcgatt aatccagcgg aattacggga gcatatagaa
      241 aactatcatc tgttactcgc tgcgttaaaa gcaaaagaca aagagggatg cagacattgt
      301 cttgctgaaa ttatgcaaca aaatattgct attttatacc agcaatacaa tcgttaaagt
      361 aaatgcccgg caaataccgg gcatcaaggt aattactcaa atctccacgc aatattcatg
      421 ccgacaccat aatttcgtcc gggcgaaggt tcgtaatatc gcccgtttga ctcattgaca
      481 atgacagaac caacgtattc tttatcgaat aaattatcga cacgaccaaa taaatccaca
      541 gttaaattgt ggtaattgta tttatacccg gtgaataagc cgacgagagt ataagacggc
      601 gcttttgccg tattttcatc atctgccata atatcgccca tataacgcgc ttccgtgcct
      661 gcataccaac catcttccgg tacataacct atcgacgcaa agcccatatt acgggcgatc
      721 cctggcatcc gattaccgtt acaatcctgt tcattgcaaa cattgctgcg ataggtcgca
      781 tcaagccagg tccaggacgc gtttacgcga aaatctcctg cgaaacgttg atcccatgcc
      841 agttcagcgc cttgacgacg ggtctttccg gcatttttgt aagtcgtacg cccaccgcta
      901 ctgctatcga caacaatttc atcatcagtg tcggtctgaa acaatgcgag actaagcagc
      961 ccatcaccaa tacgcgtttt actgccgatc tcaattgtat cgttggtgga tggttttaaa
     1021 ccaaagttca taccgctttg cccatcagca cgataagaca gctcattaat cgtcggcgtt
     1081 tcaaaacctc gcccggctgc cagatagata ttccaggcat cggtcattgc atattttaac
     1141 gaaccggcag gtagccattt atgataactg gcatcaccgc tgtcatcgcc gttacccgga
     1201 gtaacgtaat ggtcgttgga atcaaaccac acggagctgt agcgcacgcc agcatccagc
     1261 gacagttttt cgctcagctg ccactgcgtc tgtaaatagg gatcgatgtt ccacatcaga
     1321 ttgcgttcgt cgcgacgcaa ctcacctttt tgcccgtact ccggcatgcc gctattcagg
     1381 cggaagttat tgtagccctt gcggttttca ctcatgtttt cgtagttcag gccggtagtg
     1441 aacgtgaccg gaacgcccag ttcaccacgg tgtgtccagc ggctgtctat cccctggtaa
     1501 tggcgttgca gggtaatcac gccgcccgca tgtgacgggt taagttgtgg tgccatgggt
     1561 attgactggt actgggtcgt ttctcgctct ccggcataca tcatcacact catatcatct
     1621 tgcgcgctca ggctacgctc atagcgcaac ccagcctgag tttgcttgat ggtttttcgc
     1681 gtgtcgtact gttctgcacg aggcgcttgt tgtggattag ccttccattc tgctttggtt
     1741 agcccacccg ggtcatctgc tttgatatcc acactattga aaatcagact taatttgctg
     1801 gcttcatcaa tgcgtacgcc cagtttggca ttggctaaat ttttctgtgc gccactatgg
     1861 tcacgatagc cgtgggtcgt aaaacgcgtg gttgagacgg tgtaatcgac atcgccaggc
     1921 tgtgtgccgt ctcccgttgc gcccgttgct ttcagcccat agcgccagct gccaaaactg
     1981 ccgtagtaac tactggcttc aatggttggt ggctgttgtc cggtctgggt ggtgacattc
     2041 attaccccac cagacgcgtt gccatacagg gcagagaagg ggccacgcag cacttccaca
     2101 ttttgcacac tgcttaaatc gatgttggat gtttgccctt gcccgtcggg catggtggcg
     2161 ggaataccgt ccacatacag gcgaataccg cgaataccgt aagtggagcg ggagccaaat
     2221 ccgcgaatcg acagctgtaa atcttgcgca tagttctgcc ggttttgtac ctgcaaacca
     2281 ggcacgtcgg tcagtgattc ggacaagtta atgcgcggtg ttgccaggcg catctcctcg
     2341 ccatccacca cgcttactgc tgctgggata tccagttctg aaaccacctg cggtgcggca
     2401 ctgacaatca tggtctgttc atcagcggca aaaacaacgg gggaaagggc aagtagtgcg
     2461 ggcaaaacgg tctgtcggac ggaaaaaatc ttcatgaaaa aagccaggtt aagaatggga
     2521 aaacgccgtt atggtaataa aattgtaaat tcatggaaaa cgaaacggca cgatacgtta
     2581 agtaattgag aaaattgtag tcgtaacggc aagaaatgct ccacatttga gaaaataatg
     2641 attaccattc ccatttataa caagagcgta acgatgatta cgcttagcga agcattgtga
     2701 agcagcaaaa atatcggttc atcaaaggga gtcgtcatgc atttacgtca tctgttttca
     2761 tcgcgcctgc gtggttcatt actgttaggt tcattgcttg ttgcttcatc attcagtacg
     2821 caggccgcag aagaaatgct gcgtaaagcg gtaggtaaag gtgcctacga aatggcttat
     2881 agccagcaag aaaacgcgct gtggctcgcc acttcgcaaa gccgcaaact ggataaaggc
     2941 ggcgtggttt atcgtcttga tccggtcact ctggaagtga cgcaggcgat ccataacgat
     3001 ctcaagccgt ttggtgtcac catcaataac acgactcaga cactgtggtt tggtaacact
     3061 gtaaacagcg cggtcacggc gatagatgcc aaaacgggcg aggtgaaagg ccgtctggtg
     3121 ctggatgatc gtaagcgtac ggaagaggtg cgcccgctgc aaccgcgtga gctggtagct
     3181 gacgatgcca cgaacaccgt ttacatcagt ggtattggta aagagagcgt gatttgggtc
     3241 gttgatggcg agaatatcaa actgaaaacc gccatccaga acaccggtaa aatgagtacc
     3301 ggtctggcgc ttgatagcaa aggcaaacgt ctttacacca ctaacgctga cggcgaattg
     3361 attaccatcg acaccgccga caataaaatc ctcagccgta aaaagctgct ggatgacggc
     3421 aaagagcact tctttatcaa cattagcctt gataccgcca ggcagcgtgc atttatcacc
     3481 gattctaaag ccgcagaagt gttagtggtc gatacccgta atggcaatat tctggcgaag
     3541 gttgcagcac cggaatcact ggctgtgctg tttaacccag cgcgtaatga agcctacgta
     3601 acgcatcgtc aggcaggtaa agtcagtgtg attgacgcga aaagctataa agtagtgaaa
     3661 acgttcgata cgccgactca tccaaacagc ctggcgctgt ctgccgatgg caaaacgctg
     3721 tatgtcagtg tgaaacaaaa atccactaaa cagcaggaag ctacccagcc agacgatgtg
     3781 attcgtattg cgctgtaata gatatggcga gggtgaaaac ggagagactt gcctgacttt
     3841 tatcacgccg catgcggcac accttcgtca gtaatctaaa tttgcccggc acgtagccgg
     3901 gcaattttgc aattagctcg ccgtttcagg cttaaacaca atttcctgtt tttcttcatc
     3961 ttcctcgacg actggcgcag tgctgtgaat ttcagcaacg cgtttgcgca caccaaacca
     4021 gcctataacc agcagaatac cgataatcgg cagcgccgcg atagtataag tcccgttcgg
     4081 gtaatcgaac gccatcagca caaggacact cagtaaaaac agtaatgtca gccaggaagt
     4141 gaagggcgcg ccaggcagtt taaaactgac atccgctgct ttgccttctt taatcgcttt
     4201 acgcaggcgc atctggcaca cgatgataaa cgcccatgaa gcgatgattc ccagcgacgc
     4261 gaagttcaac acaatctcaa atacgcgcga cggcaccaga tagttgagga atacgccgac
     4321 gacatacaca accagtgtcg ccagaatccc ggcatacggc acatgctgac gactcatttt
     4381 cgccataaaa ctcggtgcgg aaccgcccat cgccatcgag cgcagaatac gcccggtgca
     4441 gtacagacct gagttcaggc tggagagggc agcggtcagc accacaatgt tcataatgct
     4501 gccgatatat ggcataccca gtttagagaa aaacgtcacg aacggacttt gccccgcctg
     4561 atacgcgctc cacggcaata acataaccag caacaccacg gagccgacgt aaaacaggcc
     4621 aatacgccaa atcacactgt taatggcttt aggcaccatg gtctgcggat ctttacattc
     4681 tccggcagct gtacccacca tttcaatgga ggcaaaagca aacactacgc cctgaatcaa
     4741 caccagcgca ggcagcaaac cgtgggggaa gaagccgcca ttatcggtga ttaaatgaaa
     4801 gccagtggtg ttgccatcca gcggctgacc actaccgagg aacactgtac ccacgaccaa
     4861 aaaggtcaca atggcgagca ctttaataag cgcaaaccag aactccatct ccgcaaacca
     4921 tttcacaccg atcatattca tggtgccaac gatggtaagt gcagcgagcg caaagaccca
     4981 ctgcggcacg ccgccaaacg caccccagta atgcatatac agagcgacgg cggtaatatc
     5041 aacaatcccc gtcatcgccc agttgatgaa gtacatccag ccagcaacat aagcggcttt
     5101 ctcacccaaa aactcacggg cataagagac aaaactgcca ctggaagggc ggtgtagcac
     5161 cagctcaccc aatgcacgca gaataaaaaa cgaaaacaag ccacaaatta aataaaccag
     5221 tgccagtgct ggacccgcca tttgcagtcg ggctcctgca cctaaaaaca agccggtgcc
     5281 aatcgcgccg ccaatggcga tcatctgtac ctggcgattg cccatcgctt tgtgataccc
     5341 ctcttcgtgg gcattaagcc agcggcgttt cgcggcgtgt tgatctgaag tgtcggtgtc
     5401 gtgtttactc attgctctcc ctgattgctt taatgaaaaa gtcatataag ttgccatgaa
     5461 caatgtttat tcatcctggc gatgatagtt atcaccatta ttctttatgg gtataaaact
     5521 atcggcgcag catcctaccc gttatcgata tacgatgcaa aacatcccct tacaatcctg
     5581 aaggggattc atacaactga cgaaaaaatg acaaatactt ttgctggtta acctgtgtac
     5641 tgtcctacac ttaatcttta aaagattgtg aggggcatat gattaaggtg tatggcgtac
     5701 ccggatgggg ctctacaatc agtgagctga tgctaacact ggctgatatt ccttatcagt
     5761 tcgttgatgt cagtggtttt gaccacgagg gaacttcacg cgagttattg aaaaccctga
     5821 acccgttgtg tcagattccg acactggcgc tggaaaatga cgagattatg acggagacag
     5881 cggcgattgc attgatggtt ctcgatcgtc gcccggacct tgcgccaccg gttgggcgcg
     5941 ccgaacgtca gcagtttcaa cggctattgg tctggctggt tgctaatgtc tatccaacat
     6001 tcactttcgc cgattaccct gagcgttggg ctcctgacgc acctgaacag ttaaagaaga
     6061 atgttattga atatcgtaaa tcgctttata tctggctgaa ttcacaattg accgctgaac
     6121 cttatgcgtt tggtgagcaa ctaacgctgg tggactgtta tctttgcact atgcgcacat
     6181 ggggggctgg gcatgaatgg tttcaggata atgccacgaa tatcagtgcg attgctgatg
     6241 ctgtatgtca gttaccgaaa ttacaagaag tattaaaaag gaatgaaatt atttgagtgg
     6301 gataccagag gattatgtta tttattattc
//
LOCUS       DAZPDU010000220         6302 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZPDU010000220 DAZPDU010000000
VERSION     DAZPDU010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6302
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.222"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..273)
                     /locus_tag="ACHURS_004009"
     CDS             complement(<1..273)
                     /locus_tag="ACHURS_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239621.1"
                     /translation="MTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNAHILE
                     HGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGECFKKEYSIV"
     gene            complement(332..4189)
                     /gene="sigA"
                     /locus_tag="ACHURS_004010"
     CDS             complement(332..4189)
                     /gene="sigA"
                     /locus_tag="ACHURS_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HFR1239622.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFLSSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGELTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASSGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(4551..5555)
                     /locus_tag="ACHURS_004011"
                     /pseudo
     CDS             complement(4551..5555)
                     /locus_tag="ACHURS_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            5540..5917
                     /locus_tag="ACHURS_004012"
                     /pseudo
     CDS             5540..5917
                     /locus_tag="ACHURS_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839879.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5956..>6302)
                     /locus_tag="ACHURS_004013"
     CDS             complement(5956..>6302)
                     /locus_tag="ACHURS_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1239623.1"
                     /translation="VPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGAW
                     LIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIAL
                     AAQSSFSQQKQN"
BASE COUNT         1571 a         1408 c         1410 g         1913 t
ORIGIN      
        1 gacaatgctg tattcttttt taaagcactc accagaaacg gtgattgtaa tgataatggt
       61 cttctcgatc gagatgtaaa tacccgtttc gttatactgt ccgtatcgga accatgagaa
      121 ctcagttgca ttccagccat gctccagaat atgtgcattt ttttgcctga tagtttcttt
      181 ttttattcgc tcatgtttaa ggcataacag gcagataaat aatgtcagaa aaagaaagct
      241 aataatgaga gcaagagcga tatagtctgt cacagggtaa tacatattct ctacggtaga
      301 agaagggccg caaacgcggc ccgggctgtt atcagaaaga gtaacggaag ttggcgttga
      361 tcgcgttatc cacgttgtat ttaccaaatg ccgatttctc aaactcaaga ccgaagcgaa
      421 gattatcgcg aatttcggcg ttgagaccaa cattcatgag aatacgaccg tctttttcac
      481 ctttgatacg tttctcaccg gacgcatcac gcagtacggt ttcaccgttg gcaaacaggt
      541 caaactggta gccaaggccg gcgcgggctg tgactttcca gtccttaccg gagaaggatt
      601 tacccacatc aacaccggta cgcccaatca gcggattaaa gtccttatcc ttcatggtca
      661 cgctcattcc gcgatcctgc cagtcgaacc gtttacctga tacggcccca taaaccagtt
      721 ctgcctgcgg ctcaatccag gagtcttctg ttacatgata gcggtagcct gcttcagcac
      781 ccacatacaa ggaatgagaa ctgtagtctt ttgttccgag tccagcaaag gtcgccgagt
      841 actcattatc atggtgaaca tacttactaa tcaggtcgat ataggcacca gagtcaaata
      901 tagcggaagc atacagacct gccccgacgg acttcgtctt gccactgaat gcattactgc
      961 tggcatgact gtcggtatac gtcatagtca gaccagtgaa aaggtcaagt ccatccagct
     1021 catgtttttt atccacacca atctgcacat gggtgtagtt gtcactgtat ccactagaag
     1081 ctgaacctgc tccgctcatg atgcgggccc atgcaccggc ctcgccattg gtgtcacgca
     1141 gatcccccat tcgtttattc aggttgttga cttctgtcag gaaggctttg taattaccgg
     1201 acatatacga ttttgctgca ccggaagatg ctgtattttc agccacctta tatccggtca
     1261 gggtccacgt ggtatgttcc tgatcctctt tcgtgctgat cacaggagat atgttgctga
     1321 aacctatggc ctgagtactg gctttaaata tctctttatt gcttcctttt ggggctgtta
     1381 tcaggtcaac gtttaatcct gagttattat ttcccacttt ctgtatatcg accgacagac
     1441 tgttattgct tccggttaac tgatttttaa caatgagttt atccgattga tttacagact
     1501 ggctcagatc agcccgcata atcacagcag tattagtggc atccagtgtg tttactgtca
     1561 cagtatggaa acccgaattc tctatgttac taaacaacaa aagactgtcg cgggttttta
     1621 atgattgcag ggctgactgt ccgtttactc tccataaggt gttatacatg ctggctgagg
     1681 cagcaggagc atcaatacct ccggtataag aagtatcaaa gccacccagc aaacctgtag
     1741 ccagcggtgt gtgtgtggtt ttgtccaggt tatcttcttt atactggtca acaccaaaca
     1801 gaatagatgc ttttttgtct gattttatat taccattgac ccaggcataa ttttgagcct
     1861 ttaacgtggc accatcttct gtcatattat atccatctga catatagatt gccgggctgt
     1921 atttattatt ttctgtaggg gtgcctgtta atgtcagggt tccggccagt tcaatagctt
     1981 tatctacata catttttccc tgggcggtaa cagtagctcc tttgttgata atcaggtctg
     2041 tattaattaa ggataatgca ccagtcatag taacagttga tttatcacca acagttattt
     2101 tgctgttttg agcactcaga ctgccgttga aactactttc gcctgctgaa tccccggagc
     2161 gaagctgttg tctgaatcca aaaccttcgc cagtaatgtt tcttcctgag aacatgtcaa
     2221 tgtatgcagt tttatttccc agaacaattt gagagttttt agcctctatg tgtcctttta
     2281 cattagcatc ccggcctata ctcagggttg cgttttctaa atttaatttc cggaaattaa
     2341 atttacgact ttcccagtcg ggctgggaaa aagaggctat ctgattgttg gacttatatt
     2401 ccgtattgta acgtttattt acagtgcttt cctggtcctt tatggcagca gaatagtctt
     2461 tttgacataa aaaaggaatt ggacagttgt tattgccttc tttaaatatg gcatgttctg
     2521 tcgcatgtcc ttgtaatgtt aactgtgcat tccggacaga gatatcgttt ttgatatcga
     2581 ctgagccatc aaagatcaga ttggcattat tgttatgttg ctgggtagta ttgataatat
     2641 gatttatgct tatattacct gatacattcc catggtacat atatgcatca gtattagtaa
     2701 gtgataatgt tgattgcttc acgtttgagt tcgttacggt tgttcccgca tctgttgctg
     2761 cgattttctg aaatgactgg tcatagccgt ttagatccag tgttccgcca tttctggtaa
     2821 aatagatttc gccattaccg ctttcgctta aagcgtcttt ggcatttatt tttaccgtac
     2881 ctttacctcc ggccatgtaa attttattga aggctttttc agcactaagg atgacagtcc
     2941 cattacctat tttaaggtta tttccctgtg ctatttttac atccagagta ccactaccta
     3001 ttttatgcag attatctccg gcaacgccct taacattcca ttcaacggtg gtattattat
     3061 caatatctat cccggcacct ttataagact tatcttttcc gtacacttta tatgtctggt
     3121 tcttatcgaa tataagcccg ccaatgccaa gatcggtatc ctgcttgagc gttaattccc
     3181 ctcctcctga gacagacagg tccttatctt ttataatcga tgataactct tgttttttac
     3241 catccagctg catagtgttt gtgaaattct tttttaaatt gtttaccaga atattattaa
     3301 ataacgagta gtttgtccag gctatattgg tactactgag aaaaccaact gtactggtaa
     3361 caccatggaa aacccatttt tgttgttctt tatcatatag ataagaacca gaaccactat
     3421 cgccaaaaat tggtgagttt ttaaaagggg ttctgttggt ataaatgtaa ctcttgtttt
     3481 tccagtccag attaaaaaga cttgcgctta acaagtcggg attatagttt tgtccaaaca
     3541 gatattgttt gccgtttaat atcgttgagg ttgttccggc acctgctctg aagccaatta
     3601 tttgttcctt accgttgtag tttacgccat atcttttcat cgcatcttcg ggagaaagtg
     3661 agaagtcggc actctccgta acccctgttg tttccacgac gaatttattc agacgatgaa
     3721 ccgaaaagtc gccagatgat actctgttac ttgctttata tgctgtctga tcccatgact
     3781 gtgttgttat tgcatgatgc tgtgtaccgt tatgagtcgc agtaacactg tacgcacctc
     3841 caattgatgt tgttgcacct ttgttggaaa cagcagaaaa atcaggtaca ggtagttctg
     3901 gaaaatgaaa tatctttcca tctttcatca ttaattgaac atttgttgca cccggtctga
     3961 actctcctct gttttgagca aggtcaagat aatcgcgggt ccatatatta tttatatcca
     4021 gcattgctgc aaatgctgtt tcgccataac ttccatatat tgaggataaa ataataccga
     4081 ggataacttt ctttcttgat gtaccgacac taactttccg ggtcagttca gaaacagcaa
     4141 ctaatccacc tgtaatatga ctatatttca gtgaataaat tttattcata cgtgaactcc
     4201 gatatttctg agttgtggtt atgggatagc atttaattac aacggggaac tatcagtcat
     4261 ggtacgtaac tgataagggt ataatgataa tcactgtcag ttatatttgt atgacagatg
     4321 taacaaaatg aaaaaaaaag ataagcacaa gtgattgatt ggtcagatat tgatcacttg
     4381 gtgtttgtat tttgaacttt gcgatcaaaa tttgatcttt gttgtttccg gtcaatcctt
     4441 tacaggtttt ctgtattatc cgggggagtt aagtctgtcg atgtccgaca gacttttgat
     4501 gatggaaaca acatgcagcg ataatgtgca tgctgttttg caggtggaac tcagaaggtg
     4561 aactggaact gtgcgaccgc accgtaagtg ttgtccgtgc cgatcaggcc tgcaacatca
     4621 aggtgaacca catcaaacgg tgagatcccc accccggccg tgaatgcact gccgtcgtta
     4681 ccggccagat tctgacggta accggcgcgc agctgtgccc atttccaggc attaaattct
     4741 gcgccaatgg cggcaaactg acggttgctg tcggaggtga aaccgctggc cggggtcaga
     4801 tccacatcaa atgcggtggt gaacatcgcg ttgtgccagg agacaccggc cgtcacctgc
     4861 gggcggatcc tgaaggtttc cgtgataccg ttcacttctt ttgtctcaat actacggggg
     4921 atcaggttct gtgcgaccag tcccagcgtc cagttgtcat ccaggtccgt actggcaccg
     4981 atgtcggcgt tgatgccatt tttcgtgttg tggtagcggt cgcctttgaa ggcgctgctg
     5041 tcataatttc tgaccagcac gttgtagttg aacaggtcga cgcgctggaa tttggggggg
     5101 aagcccagag accatttctg cccggccgtt tccagttccg tagccagagc aatacccaca
     5161 tcagtgatca gtgccgcgcg ggcaaaggcc tgtgatgtca ggcggctttt gtccacctcc
     5221 tgcccggtgc tgtttgccac ctcttccagg taagtgaggt cagcatcaga gactttaccg
     5281 tcgacactga cggtgccgtg tgatttaacc atcagtgcgg cagagaggcg atcaccggga
     5341 agggccgcca cagcagaaac gcccagctgt gcggcggcat gtgtgttacg gaagtcccgg
     5401 aggtgttctt tcaggcgtgc ggcagcctca ggaacaccgt ggttactttc gatggcgcgg
     5461 tcgaaggcct tccactcatc actgatccgg tcggcgttgt cggtgatatt atccggattg
     5521 gagagctgcg cgccgacgga cggaagcagg atgctgggct catgcgcgac gcaaaatcca
     5581 cgatgtatat atcagtacta aaagcgcgac ggcggaagaa gctctgaaac taatcggtga
     5641 actgtacgcc atagagcacg atatacgcgg gttaccggtg tctgaacgcc tggcggtcag
     5701 gcaaatgcag agtagaccgc tcctgacttc cctgtataag ctgatgcagg agaaagaaca
     5761 cacgttatcg aagaaatgtc gtctgggaga tgcgttccgg tatatcagga agcactgggc
     5821 tgcgttgtgc aacttctgtg atgatggcct ggctgaggta gataataatg cagcagaaag
     5881 agcgcttcgt gcagtctgtc tcgggaagaa aactttttcg aggatcactc attccgtgtc
     5941 tgactgcttc agggtttaat tttgcttctg ctgactgaat gatgattggg cagcaagtgc
     6001 aatatcccac ataaatccag ttaactcacg agccaccgcc gtaatggcta cttgtgattt
     6061 tttccctttt gctgtcagag ttcggtaacg agaacacaat cgttgttgag ctttccatgc
     6121 aatatctttg gaatattgtg ttacagagtc cggtcggtaa tatataagcc atgctccaac
     6181 ttttgcaggt gtacgataag accaagcggc ttcgtaaagc agacgtctca gttcgctgtt
     6241 tccaactttt gttattcctc tgggacgaat actgtttcca ctggaatatt cacctggcac
     6301 ca
//
LOCUS       DAZPDU010000221         6261 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZPDU010000221 DAZPDU010000000
VERSION     DAZPDU010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6261
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.223"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="ACHURS_004014"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="ACHURS_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR1239624.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAVFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="ACHURS_004015"
     CDS             2219..2767
                     /locus_tag="ACHURS_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFR1239625.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="ACHURS_004016"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="ACHURS_004016"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFR1239626.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3663..4853)
                     /gene="aspC"
                     /locus_tag="ACHURS_004017"
     CDS             complement(3663..4853)
                     /gene="aspC"
                     /locus_tag="ACHURS_004017"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFR1239627.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(5038..6126)
                     /gene="ompF"
                     /locus_tag="ACHURS_004018"
     CDS             complement(5038..6126)
                     /gene="ompF"
                     /locus_tag="ACHURS_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFR1239628.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVELVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         1555 a         1548 c         1595 g         1563 t
ORIGIN      
        1 ttacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgccgt tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgcgac gtagtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccggtcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcacttg caacgccgcc gctctcggtg attaaccaat ggcagctggc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggcctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gcgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggggcaga
     1201 tggtgctatt ggcccggcaa cacgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggct ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccggcct attcgctggt ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcccttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt acctccaact ctggcacgca aagatattct
     1501 gccaaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg gcaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg ttccgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggctg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atggtcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca gttgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga cctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttctatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattatccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa ttgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attaatctgg tgtgaacaaa cccgtctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc cacacttggt tatgaacgcc tgcataatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aggcacataa aaaagcccgc ttttaatgcg ggcctggatt
     3481 tctggcaaag tgcgctttgt ttatgccgga tgcggcacga gcgccttatc cggcctacaa
     3541 aatcgtgcaa attcaaaata ttgcagggga cgcgtaggcc tgataagcgt agcgcatcag
     3601 gcaatgttgc gtttgtcatc agtctcagcc cgcttttcag cgggcttcat tgtttttaat
     3661 gcttacagca ctgccacaat cgcttcgcac agcggagcca tgttatctgg tgtcatcccg
     3721 gccacattta cgcgaccaga agcaaccgca tatacgccaa actcttcgcg cagacgcagc
     3781 acttgttctt ttgtcaggcc actgaaggag aacatgccgt tctgtttgat gataaagctg
     3841 aagtcgcggt ttgcgccttt ttcctgcagc gtattgacga acaactgacg catacgctga
     3901 atacgctggc gcatatcagt cagctcttgt tcccaaatcg cacgtaacgc atcgttgctc
     3961 aggatggtgg caacaacaga agcgccgtgt gctggtgggt tagagtagtt agcgcgaatc
     4021 gccgctttca tttggctgaa tgcgcgatca acggtttcac tgtcggcagc aaccagagta
     4081 caagcgccaa cacgctcgtt gtacaggcca aagtttttag agtaggaact ggcaacaatc
     4141 agctctttat gcatagccgc gaaagcgcgc agtccttcag catcttcttc cagaccacgg
     4201 gcaaaaccct ggtaagcgaa gtcaaacagc ggtaaccagc ctttctcaac ggagagttgt
     4261 gccagtgtct gccattgttc cagcgtaggg tcgataccgg ttgggttatg gcagcagcca
     4321 tggaacagca ctacgtcgcc agcctgagct tcattcaggc tgttaatcag tgcatcgaag
     4381 tcaagagtgt gattttccgc atcataataa gcgtattcac gaacttccag acctgcagag
     4441 ttaaagacgc tcttatggtt cggccagctt gggttgctca cccacactcg cttaacgctg
     4501 gtattttttg ccaggaaatc ggcagctacg cgtagtgcgc cagtgccccc cggagtctgt
     4561 gccgtgcgag cacgtttgtc attgatcagg gcgctacctt taccaaacag cagttcctga
     4621 gtgcagcgac caaattcagg gatgccgtca atgccgaggt aatttttggt ggtttcattt
     4681 tcgagcagat actgttcagc ctttttcacg ctggtcagta ccggggtttt gcccgtctca
     4741 tctttataga caccaatccc gaggttaatt ttgccgggac gttcatcggc acgaaacaga
     4801 tcggccaggc ccagaatcgg gtcggcagga gcggcggtaa tgttctcaaa catgacgagg
     4861 ttccattatg gttacagaag ggaagtccgc tatcagggta acgggagatt tacaaaattc
     4921 caactattac tgatgaaaac gcaggctgtt tttgcaagac gtgagattgc tctggaaggt
     4981 ataaaaaaaa caggaccaaa gtcctgtttt ttcggcattt aacaaagagg tgtgctatta
     5041 gaactggtaa acgataccca cagcaacggt gtcgtctgaa cctacgccca gtttgttgtc
     5101 agaatcgatc tggttgatga tgtagtcaac ataggtggac atgtttttgt tgaagtagta
     5161 ggttgcgccc acttcaaagt agttcaccag ctcaacatca ccgatacctt ctacgtcttt
     5221 cgctttagat ttggtgtaag cgatggacgg acgcagaccg aaatcgaact ggtattgcgc
     5281 aactaacaga acgtcttgcg ttttgttggc gaagccgctg gtgtttgtaa atttattagt
     5341 gatcggcgta gcgttacggg tttcaccgta gttcgctgcc aggtagatgt tgttcgcgtc
     5401 gtacttcaga ccagtagccc actgttcagc ttttttaccg ttgccaagag gttgagcttc
     5461 ttgcaggttg gtacggtcag ctgcaccata agcaccaacg ataccaaagc cttcgtattc
     5521 gtagctgata gaaccgccaa caccgtcgcc gttagaacgg cgtgcagtgt cacgctcgtt
     5581 tttacccagg tactgaacag cgaagttcag gccatcaacc agaccaaaga agttggagtt
     5641 acgataggta gcaacgccgc caacacgacc aacgaagaag tcatcgctgt atgcagtatc
     5701 accaccaaat tctggcagca tatcggtgta acccagtgca tcataaacca caccgtagtt
     5761 acggccgtaa tcgaaagaac caacgtcagc gtatttaaga cccgcgaatg ccagacgcgt
     5821 tttgttacca gtttgagcgt cagcgccttc agagttgtta ccctggaagt tatattccca
     5881 ctgaccataa ccggtcagat cggaattgat ttgagtttcc cctttaaaac caagacgggc
     5941 ataggtcatg tcgccattgc caccgtaact gttttcaccg ttacccttgg aaaaataatg
     6001 cagaccaaca gctttaccgt acagatctac tttgttgcca tctttgttat agatttctgc
     6061 agcgtttgca gtacctgcta ctaacagagc agggacgatc actgccagaa tattgcgctt
     6121 catcattatt tattaccctc atggtttttt ttatgacacc tgccactgcg tcaataagtt
     6181 ctgtcaataa aaatttacgg aactattgat gagagtttgg tgtctttatg tgtctgcagg
     6241 catctttcca ttcaaactaa c
//
LOCUS       DAZPDU010000222         6215 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZPDU010000222 DAZPDU010000000
VERSION     DAZPDU010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6215
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.224"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(10..165)
                     /locus_tag="ACHURS_004019"
     CDS             complement(10..165)
                     /locus_tag="ACHURS_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFR1239629.1"
                     /translation="MNTIDLGNNESLVYGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(368..775)
                     /locus_tag="ACHURS_004020"
     CDS             complement(368..775)
                     /locus_tag="ACHURS_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1239630.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            852..1079
                     /locus_tag="ACHURS_004021"
     CDS             852..1079
                     /locus_tag="ACHURS_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFR1239631.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPWKVIHENQA"
     gene            1063..1484
                     /locus_tag="ACHURS_004022"
                     /pseudo
     CDS             1063..1484
                     /locus_tag="ACHURS_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
     gene            1562..2361
                     /locus_tag="ACHURS_004023"
                     /pseudo
     CDS             1562..2361
                     /locus_tag="ACHURS_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2368..3114
                     /locus_tag="ACHURS_004024"
     CDS             2368..3114
                     /locus_tag="ACHURS_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR1239632.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRSE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYQVQNDGQRYALSQAKSIADELMTGCT
                     NFAFSGKLGTGKNHLAAAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCEVDLLVLDEIGIQRETKNEQVVLHQIVDRRTASMRSVGMLTNLNYEVMKTLLGERV
                     MDRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            3129..3551
                     /locus_tag="ACHURS_004025"
     CDS             3129..3551
                     /locus_tag="ACHURS_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR1239633.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            3609..3965
                     /locus_tag="ACHURS_004026"
     CDS             3609..3965
                     /locus_tag="ACHURS_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239634.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            4134..4799
                     /locus_tag="ACHURS_004027"
     CDS             4134..4799
                     /locus_tag="ACHURS_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFR1239635.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
     gene            5027..5182
                     /gene="hokD"
                     /locus_tag="ACHURS_004028"
     CDS             5027..5182
                     /gene="hokD"
                     /locus_tag="ACHURS_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR1239636.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            5432..5719
                     /locus_tag="ACHURS_004029"
     CDS             5432..5719
                     /locus_tag="ACHURS_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239637.1"
                     /translation="MKIEELREIFSENGLYAVRVENGAIVSHCRIRCLQSQQRKSGAV
                     LFYFCNGLLTDGFILREDEFVTSLRVLKEIGFKAGFLLLLKNKLIYNLEQD"
     gene            5758..>6215
                     /locus_tag="ACHURS_004030"
     CDS             5758..>6215
                     /locus_tag="ACHURS_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFR1239638.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVCN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTSDTALLKSFEAFREWVAIQAGFYTEHFYPDGSRGCRAKSIA"
BASE COUNT         1644 a         1349 c         1690 g         1532 t
ORIGIN      
        1 tcgttcattt tactcacctg aatgtcttcc caaccaacga cgtgcgccag cttcggtttt
       61 aaacgttttg cttttggtat acgtcatggc ggtgaatgtg ccgtcctggt tggggaacac
      121 gccgtacacc agagattcgt tgttgccaag atcgatagta ttcatgttga ccccatttcc
      181 ccttaacgcc ggggtagcgg aactgtttgc tgagaacacc gtgcggtgtc ttgatgaatg
      241 aaatttagaa taacctaagg tgggcggtca agatttttgt gtagaaaaac ctaagttttt
      301 tgatgtaaaa aacacaagtg tttgaaagtt tgtgcttttt attacagggt gtggagaaaa
      361 aaggggatta tttgtttgcg cttcttttgc gagctttgag tagttcttca aaaagtttgt
      421 tgaaattttc aactcgagca cgcatctctg acaacagggc cttttgctct gactcaggca
      481 gtgcgtcgaa cagttgaagt aactcttttt gatcttctgt cagaatggct ggctgattat
      541 ccgggatcgg ttcgcctggt tgtttatctt catccccaaa aagaagccaa gtcggtgagc
      601 actgaagcgc ttggctcagt gcgaataatc ttttccccgc cggctgtgtt tcatctcttt
      661 cccattgaga aattgttacg tgagccactt tgaccagctt acctaatgcg gcctgagaca
      721 gttttaattt tttacgccta tgtaagaggc gagcaccgaa ggtttcgttt ttcatattag
      781 ggaattctaa tttttcttga cttaggtttc tctacgatct agtttcctta ggaaaatcta
      841 aggagctcga tatgttgaaa attgatgcta tagcgttttt tggcagcaaa acaaagcttg
      901 ccaatgccgc aggagttagg ctggcaagca ttgctgcatg gggggaactg gttcctgaag
      961 gtcgcgcgat gcgcctgcaa gaggcatctg gcggggaact tcagtacgac cccaaagttt
     1021 atgacgaata tcgtaaggca aaacgacctt ggaaggtgat tcatgaaaat caggcatgag
     1081 cacatcgaat cagtgctgtt gccctggcag ccgaaaaagg gcaggcgtgg gtcgctaacg
     1141 caattactga agaatatctg cggcaggggg gcggcgaatt gtctctggta ccaggcaagg
     1201 actggaacaa tcagcagaat atctatcacc gttggttgaa aggtgaaacg aaagcgcaaa
     1261 gggaaaaaat tcagaaactg atccctgcgg ttctggcaat tcttccgcgt gagctgcgtc
     1321 accgactctg tatcttcgat accctggaac gccgtgcatt actggcggcg caggaagcgt
     1381 tgagtacggc aattgatgcg catgatgatg ctgtccaggc cgtttaccgg aaagcacatt
     1441 tcagcggtgg tgggtcgccc ggcgattctg tcgtagtgca ttgattgaaa ttaatcgtgc
     1501 cggattgttt tgttcggtat cagttaaatg taacgctgcg agcgttacaa ggtgaaaaca
     1561 aatggcttca aactggataa agctcgaggt tattacgccg gataagccgg aaatattcag
     1621 gcttgctgag attctgaata ttgatccaga tgccgcatta gggaaggtta ttcgcttctg
     1681 ggcatgggcg gatcaacaaa tgatagacgg taatgcagat tgtaacgctc gcggcgttac
     1741 aaaaagtgca atagatcgca tcacttttat gtctggtttt gctgatgcgt taattcaggt
     1801 tggatggctg gtcgaaaatg acggtgggct ttctctacct aactttgaac gtcataacgg
     1861 aaaaagctct aaaaaaaaac gggcggttac aaacgagcga gtaacaaaaa tacgcgaact
     1921 gaaacgaaaa ggtaacgctg ccagcgttac acaaacggat caaaaagcgt taccagagga
     1981 agaggaagag gaagaggaag aggaagagga agatctaaat actgatctcc ccctcgccaa
     2041 aaacgagcgt ctaaaaaatt cgagccggag gctattgagc tgcccgattg gttgccggaa
     2101 acactctggc atgagtgggt ccggttcaga caggcattgc gaaaaccgat tcgaacggag
     2161 cagggcgcta acggggcgat acgggaactg gaaaaattcc gtcagcaggg ttttacacct
     2221 gagcaggtga ttcgacacag catcgccaat gaataccagg gcctgttcgc gccgaaaggt
     2281 gttcggcctg agacgttgct ccgacaggtt aacaccgtct cgttgccgga cagtgcgatc
     2341 ccgccaggct tcagggggta acggaccatg aaaaatattg cgacaggcgg cgttctggaa
     2401 cgcatccgcc gactgacccc gccacatgta accgccccat tcagaacggt agcggagtgg
     2461 cgcgagtggc aacttgctga aggccagaaa cgtagcgagg agatcaaccg cctgaatcgc
     2521 cagttgcggg tggaaaaaat tctgaatcgc tcaggcatcc agccgttgca ccgtaaatgc
     2581 tcgtttgcga attaccaggt gcagaacgac ggccagcgat acgcgttaag ccaggcgaaa
     2641 tccatcgccg atgaactgat gaccgggtgt acaaattttg cgttcagcgg aaaacttggt
     2701 accgggaaga atcacttagc ggcagctatc gggaatcgcc tgctgaaaga cggtcagaca
     2761 gtgattgtgg ttaccgtggc tgatgttatg agcgccctgc acgccagcta tgacgacggg
     2821 cagtcaggcg aaaaattttt gcgggagctg tgcgaagtgg atctgctggt tcttgatgaa
     2881 attggcattc agcgcgagac gaaaaacgag caggtggtgc tgcaccagat tgttgatcgc
     2941 cggacagcgt cgatgcgcag cgtggggatg ctgacaaacc tgaactatga ggtgatgaaa
     3001 acattgctcg gcgagcgggt gatggatcgc atggtcatga acggcgggcg ctgggtgaat
     3061 tttaactggg agagctggcg tccgaatgtt agccattcga gggttgttaa gtagtttcag
     3121 gaggatttat ggcgaaacct tttactcccg aacagcggga agaactgaag acgcgaattg
     3181 tggaactcgt gcatcaggac ggtcgggtca cgattcggca gttgtcagat gaaacaggta
     3241 tcagtcgtgc gtctgtcggt cgcttatgca tagaactggt cgcaagtggt gatgtatata
     3301 attctggcta cggcttattc ccgtctgaac aggctcgcaa ggactggcaa agcgcccgca
     3361 aaaaactctc gagagtaaag gtgaggaaac cggttgttgt tgatccggac cttatctggt
     3421 cattacctga cggagaaata cgccgctacg acaggcgcct gaatataatc tgtcgcgagt
     3481 gccggaagag cgaagctatg cagcgtgtac tggctttcta tcagggtaat tttcaggagg
     3541 cgatactgtg aatgaaatta gctatcaggc ttcaattacc gctggcattc gcatcaaagg
     3601 agaggagcat ggaaataaaa ccagaagatg agttaagcaa tatcgtttta tttccggtaa
     3661 aagaggatga ccctcgtaat caggttaatt ttctttatga gccatcggaa agaccatatt
     3721 gccatcacgc ctctgtacgg gttgacgaaa aagagcgtca ggtccgctgt aaaatctgcg
     3781 gtgcggttgt ggagccgttt gactggatgc tctctgtggc aaaaagagaa accagactgg
     3841 cagatgatgt aaggctattg cgccaggagg aacaggaaag gcggagaaat atagaaaagc
     3901 tgatacagat tgagcgtaac gcgaaagcgc ggatacgcag ggcgacaaaa tccagaactg
     3961 aataattaaa tttagcactg ttaaaaattt aatccttaac cggagggatt tctgcaccct
     4021 caaatcatca ggagaccacc cgaaagggcg gggagcagtc acacatctgt ttccgatagc
     4081 cccgttctaa tgctacactc tttgatattt ttatgacccc aataaacata tttatgacag
     4141 ttgctgattt caaacggccc aaattggagc tcccaaacgg ggcaaacaaa ctactactgc
     4201 actcttgctg tgctccatgt tccggtgagg tgatggaggc gcttcaggcc tcgggaatcg
     4261 actacaccat ctttttctac aacccgaaca ttcatcctca gaaagagtat ttaattcgta
     4321 aggatgagaa tattcgcttt gctgaacaac acggcgtgcc gtttattgat gctgattacg
     4381 acacagacaa ctggtttgaa cgagccaaag gaatggaatg ggagcccgaa cgagggatcc
     4441 gttgcaccat gtgttttgac atgcgttttg agcggacagc gctgtacgcc gctgaaaatg
     4501 gtttcagtgt gatcagcagt tcactgggca tttcacgctg gaaaaatatg cagcaggtta
     4561 acgactgtgg gcggcgagcc gtcgcgcatt atccgggcat ggtgtactgg gattataact
     4621 ggcgcaagca gggcggctcg tcccgtatga ttgaaatcag caagcgcgaa aaattctatc
     4681 agcaggaata ttgtggctgt gtgtattctc tgcgcgatac caatctacac cgcaaatctc
     4741 agggacgccc tcttatcaaa attggtcaac tccactacgg taaagaagag aaggagtgat
     4801 tttatggggc acctttctga ttgatttcat attggcgagg taagtagaat gactgcgggt
     4861 gcttgaggct atctgcttca ggcatgaaca ccaaaaggca gatagagaaa agccccagtt
     4921 aacattacgc gtccggcaag acgcttaaca ttaatctgag gccaatttca tgctttgcac
     4981 atgtaggtta gcctcttaca tgccgaaagg caaggagaag caggctatga agcagcaaaa
     5041 ggcgatgtta atcgccctga tcgtcatctg tttaaccgtc atagtgacgg cactggtaac
     5101 gaggaaagac ctctgtgagg tacgaatccg aaccggccag acggaggtcg ctgtcttcgt
     5161 agactacgaa tccaggaagt aagagtgacc gggcggggag ctgatcccat ccccgcccac
     5221 ctctgatgtg tcaggcatcc tcaacgcacc cgcacttaac ccgcccatcg ctgtgatctc
     5281 tcagcgtttc ggcgggtttt ttgttgttta tttccggtga atttgattcg cgcaccttcg
     5341 cagatagaat cgactcactt aagtagcgcg cagggagaag agggatggac cccgaacagg
     5401 ggagagctat ttatctggaa ggattctgaa gatgaaaatc gaagaattgc gtgaaatttt
     5461 tagtgaaaat ggcctctatg ctgtgcgcgt tgagaatgga gcgattgtca gccattgccg
     5521 cattagatgt ttgcaatctc aacaaaggaa gagcggtgct gtgttatttt atttttgtaa
     5581 tggacttctg acggacggtt ttattttgcg tgaggacgaa tttgtcacat cattacgggt
     5641 tttgaaagag attggtttta aggctggttt tctgcttttg ctgaagaata aactcatcta
     5701 caatcttgag caggattgaa ctcctgctgt gtaacaccgt gccaccggag aaagccgatg
     5761 gcacatatac aactggtcaa acaaacctct tccggattac ttctcccggc gacgccggag
     5821 agttgcgatt ttctgcatca aatcaaaata ggtgagtgga tacacgcaga ctttaagcgt
     5881 gtgtgtaact acgcattcca caagcgtttt ttcaaactcc tgcaactggg tttcgattac
     5941 tggactccgg tcggtggggc gatcacgcct cgcgaacgaa aactggtgtc cggtttcgtt
     6001 gattacctgt gtgaatcagt aggccgggaa catacgccag ctctgagcga agccgcagag
     6061 caatatctga atacagttgc gacacgcaga accagtgata cggcattgct aaagtcgttt
     6121 gaggctttcc gcgagtgggt agccattcag gccggatttt acaccgagca tttttatccg
     6181 gacggtagcc gtgggtgtcg ggcgaaatct atcgc
//
LOCUS       DAZPDU010000223         6151 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZPDU010000223 DAZPDU010000000
VERSION     DAZPDU010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6151
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.225"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            76..852
                     /gene="exuR"
                     /locus_tag="ACHURS_004031"
     CDS             76..852
                     /gene="exuR"
                     /locus_tag="ACHURS_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFR1239639.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1092..1175
                     /gene="yqiM"
                     /locus_tag="ACHURS_004032"
     CDS             1092..1175
                     /gene="yqiM"
                     /locus_tag="ACHURS_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFR1239640.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            1197..1859
                     /gene="yqjA"
                     /locus_tag="ACHURS_004033"
     CDS             1197..1859
                     /gene="yqjA"
                     /locus_tag="ACHURS_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFR1239641.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPIFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1863..2246
                     /gene="mzrA"
                     /locus_tag="ACHURS_004034"
     CDS             1863..2246
                     /gene="mzrA"
                     /locus_tag="ACHURS_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFR1239642.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2393..2761
                     /gene="yqjC"
                     /locus_tag="ACHURS_004035"
     CDS             2393..2761
                     /gene="yqjC"
                     /locus_tag="ACHURS_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFR1239643.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            2799..3104
                     /locus_tag="ACHURS_004036"
     CDS             2799..3104
                     /locus_tag="ACHURS_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFR1239644.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3107..3511
                     /locus_tag="ACHURS_004037"
     CDS             3107..3511
                     /locus_tag="ACHURS_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFR1239645.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3501..3800
                     /locus_tag="ACHURS_004038"
     CDS             3501..3800
                     /locus_tag="ACHURS_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFR1239646.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3986..4378
                     /locus_tag="ACHURS_004039"
     CDS             3986..4378
                     /locus_tag="ACHURS_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFR1239647.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4448..5433
                     /gene="yqjG"
                     /locus_tag="ACHURS_004040"
                     /pseudo
     CDS             4448..5433
                     /gene="yqjG"
                     /locus_tag="ACHURS_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
     gene            5726..6091
                     /locus_tag="ACHURS_004041"
     CDS             5726..6091
                     /locus_tag="ACHURS_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR1239648.1"
                     /translation="MDWYLKVIKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
BASE COUNT         1542 a         1514 c         1548 g         1547 t
ORIGIN      
        1 tatatatgac caatggcata cccatgaata aagtggtatg ataacttcgt ccgcattcca
       61 ctccgggaga cgcatatgga aatcactgaa ccacgccgtt tgtatcaaca acttgccgct
      121 gacctgaaag agcgcattga acagggcgtc tatctggtgg gtgataaact gcctgccgaa
      181 cgctttattg ccgatgaaaa gaacgtcagc cgaacggtag ttcgtgaagc catcatcatg
      241 ctggaagttg aaggctatgt ggaggtgcgt aaaggttcag gtattcatgt ggtttccaac
      301 cagccgcgcc atcagcaggc ggctgacaat aatatggaat tcgccaatta cggtccgttt
      361 gagttgcttc aggctcgcca gctcatcgaa agtaatattg ccgagttcgc ggcaactcag
      421 gtaacgaaac aagacatcat gaaactgatg gccattcagg aacaggcgcg cggcgaacaa
      481 tgcttccgtg attccgagtg ggatttacag ttccacattc aggtagccct ggcgacgcag
      541 aactccgccc tggcggccat cgttgaaaaa atgtggaccc agcgtagcca taacccgtac
      601 tggaaaaaac tacacgaaca cattgattcc cgtaccgttg ataactggtg tgatgaccac
      661 gatcaaattc tcaaggcgtt aattcgcaaa gatccccatg ctgctaagct ggcaatgtgg
      721 cagcatctgg aaaacaccaa aatcatgtta tttaacgaaa ccagcgacga tttcgagttc
      781 aatgccgacc gctatctatt cgccgaaaac ccggtagtgc atctcgatac ggccaccagt
      841 ggcagtaaat gaaactcctg tccgacaggc gctgcgacgc gcctgttcgc ttcgtcggta
      901 agcgaaaagt ataaagtgtc agcctgtgta aatcctctcg ccaccctccc ctgcattcag
      961 caaaatcaga ctccacggac atgtaatttt gataacgaac aacgttgacc tttgttacaa
     1021 ttagattcaa tttgaattta tgtttttgaa tgctttctta tctcacgatt taacagggaa
     1081 tagttcaggc tgtgttgatg tatcaaaccc gcagaacata ccaaaacagc aataacattg
     1141 cggtagtgca tcttttaaaa ccagcgtggc gttaaccgat tcaccaggaa taatgaatgg
     1201 aacttttgac ccaattgctg caagccctgt gggcgcagga ttttgaaacc ctggccaatc
     1261 catcgatgat tggcatgttg tattttgtct tgtttgtaat tttgttcctt gaaaacggct
     1321 tgcttccagc ggccttttta ccgggcgaca gtttactggt attggtcggc gtgttgattg
     1381 cgaaaggcgc gatgggttac ccgcaaacga ttctgctgct gaccgttgcc gccagcctcg
     1441 gctgctgggt cagctatatt caggggcgat ggctgggcaa tacccgcacc gtacaaaact
     1501 ggctatctca tttacccgcg cattatcatc aacgcgcaca ccatcttttt cataaacacg
     1561 gtttatcggc gctgttaatt ggtcgcttta ttgcgtttgt cagaacactg ctgccgacga
     1621 ttgccgggtt atcagggctg aataacgcgc gctttcagtt tttcaactgg atgagcggtc
     1681 tgctgtgggt attgatcctg acaactctgg gttacatgct cggcaaaacg ccgatatttt
     1741 taaagtacga ggaccagctg atgtcatgcc tgatgctgct cccggtggtg ctgctggtgt
     1801 ttggcctggc aggttctctg gtcgtgttat ggaaaaagaa atatggaaat cgggggtaag
     1861 ggatgcaaat acctcgcatg tcgcttcgcc agctagcctg gtccggcact gttttacttc
     1921 tggtcggcac gctgttgctg gcctggtccg cggttcgcca gcaagagtct acgctggcga
     1981 ttcgtgccgt tcatcaaggc acaacgatgc cagacggttt ttcgatctgg catcaccttg
     2041 acgctcatgg cattcctttc aaaagtatca cccccaaaaa cgacactctg ttaattacat
     2101 ttgactccag cgaccagagc gccgccgcaa aagcggtcct cgacagaaca ttgccccatg
     2161 gctacatcat tgcgcagcag gacaataaca gtcaggctat gcagtggctg acccggttac
     2221 gggataactc tcatcgcttc ggataacttc ctgttatccg aaacatatca ctcactttgg
     2281 tgatttcacc gtaactgtct atgattaatg aggcggtggt taacactacc gcttcgttgt
     2341 ctggacaatc gttcctttgt aataggtccg aatcacaatg gaaggttcaa gaatgaaata
     2401 ccgcatcgct ttagctgttt ctctctttgc tcttagtgcc ggtagttatg ccactaccct
     2461 gtgtcaggaa aaggagcaaa atatccttaa ggagatcagc tatgccgaaa aacaccaaaa
     2521 ccagaatcgt attgacggtc tgaataaagc cctgagtgaa gtccgggcca actgttcaga
     2581 tagccagctg cgtgccgatc atcacaagaa aatcgcaaag cagaaagatg aggtggcgga
     2641 acgccagcaa gatttagccg aggcgaagca aaaaggcgat gccgataaga ttgccaaacg
     2701 cgaacggaaa ctggcagaag cgcaggaaga gctgaaaaag ctggaagcgc gcgactacta
     2761 actcacaata gtcactactt actcacctgg agaaaactat gtcgaaagaa cacactacgg
     2821 aacatctgcg tgctgagttg aaatcccttt ccgatacgct ggaagaggtg cttagctcat
     2881 ctggcgagaa gtcgaaagaa gagttgagta agattcgtag caaagcggag caggcactga
     2941 aacagagccg ttatcgcctg ggtgaaaccg gtgatgccat cgccaaacaa acccgtgtcg
     3001 cggcggcgcg tgctgatgag tatgtgcgtg aaaatccgtg gaccggcgtg ggaattggcg
     3061 ctgcaatcgg tgtagtgctc ggcgttctgc tgtcgcgtcg ttaattatgg cggacactca
     3121 tcacgcgcaa gggcccggta aaagcgttct gggcatcggg cagcgaattg tttccatcat
     3181 ggttgaaatg gtggaaacac gtctgcggct ggcggtggtg gagctggaag aggaaaaagc
     3241 taatctcttt caacttttac tgatgctggg cctgacgatg cttttcgctg catttggtct
     3301 tatgagcctg atggtgctaa ttatttgggc ggttgatccg caatatcgcc tgaatgcgat
     3361 gattgccacc accgtggtgt tgctgctact ggcactgatt ggcggtatct ggacgttacg
     3421 taaatcgcgt aaatccacct tgctgcgcca tacacgccat gagctggcaa acgatcggca
     3481 actgctcgag gaggagtccc gtgagcagta aagtcgaacg tgaacgacgt aaggcgcaac
     3541 tgcttagcca gatccagcaa caacggctgg atctttccgc cagtcgtcgt gaatggctgg
     3601 aggcaacagg cgcttacgat cgtcgctgga atatgctgct aagtctgcgc tcctgggcgc
     3661 tggttggcag tagcgtgatg gcgatctgga cgattcgcca tcctaatatg ctggtccgct
     3721 gggccagacg cggttttggc gtatggagcg cctggcgtct ggttaaaacg accctcaagc
     3781 agcaacagct tcgcggttaa cttccctctg gccggagcaa ttccggcctt atccctcaaa
     3841 ttttttgaag atttttgaca gttttccttg ctaacaatca tcattcacca cgtttatgat
     3901 tctctccatc gacagcaacg acgctaatac cgcgcccttg cacaaaaaaa caatcagcag
     3961 cctgagtggc gcagtggaga gtatgatgaa aaaattagaa gatgttggtg tactggtagc
     4021 gcgcatttta atgccgattc tgtttattac cgcaggctgg ggaaaaatca ccgcttactc
     4081 tggtacccaa caatatatgg aagcaatggg tgtcccgggt tttatgctgc cgctggtgat
     4141 tctgcttgag ttcggtggtg gtctggcaat cctgttcggt ttcctgactc gcaccacagc
     4201 cctgtttact gcgggcttta cgctgctgac ggcattttta tttcacagca actttgctga
     4261 aggcgtcaac tcgctgatgt tcatgaaaaa cctgacaatt tctggcggat tcctgctgct
     4321 ggcaattacc ggtccgggcg cgtatagcat cgaccgcctg ctgaataaaa agtggtaagc
     4381 acgcgctata cttaacgata aaaaacgagg aggaagctcc tcgtttttgc tattggagga
     4441 gagaaaaatg ggtcaactga ttgacggcgt ctggcatgac acctggtacg ataccaaatc
     4501 taccggcggt aaatttcaac gttcagcttc cgcatttcgt aactggctca ctgccgatgg
     4561 cgcacctggc cccactggca caggcggttt tatcgcagag aaagatcgtt atcatctcta
     4621 tatttcactc gcctgcccgt gggcgcaccg cacgctgatc atgcgcaaac tcaaaggact
     4681 ggaaccgttt atttccgttt ccgtagtgaa cccgctgatg ctggaaaacg gctggacctt
     4741 tgatgacagt tttccgggag caaccggaga tacgctctat caacatgaat ttctgtatca
     4801 gctttatctc cacgccgatc cacactacag cggacgagtt actgttcccg tactgtggga
     4861 caaaaagaac cacaccatcg tcagcaacga atcaacagaa atcatccgca tgttcaatac
     4921 cgcgtttgat gcgctgggcg cgaaagcggg tgattactac ccaccagccc tgcaaacgaa
     4981 aattgacgaa cttaacggct ggatttatga caccgttaac aacggcgtgt ataaagctgg
     5041 ttttgccacc agccagcaag cctacgacga ggcggtggcg aaagtgtttg aatcgctggc
     5101 gcgactggag cagattttag gccagcatcg ttacctgacc ggcaaccagc taaccgaagc
     5161 cgatattcgc ctgtggacta cgctggtgcg ttttgatcca gtgtatgtga cccacttcaa
     5221 gtgtgataag caccgcatca gcgattacct gaatctgtat ggcttcctgc gcgatatcta
     5281 ccagatgccg gggatcgccg aaactgtcaa tttcgatcac atccgtaatc attacttccg
     5341 cagccataag accatcaacc ctacggggat tatttcaatt ggtccgtggc aggatctcga
     5401 tgaaccgcag gacgagatgt tcgcttcggt taaaaaaagg caccttacga agtgcctctg
     5461 ataattagct aagtgcatgt tgttcatatc ggatgcggcg tgaacgcctt atcaaaccta
     5521 caaaacattt atattcaata tgttgcaatg accgcgcagg ccagataagc gtagcgcatc
     5581 tggcgatttt gcgcttgtca tcagtcttag cgcgccttca cagcacttta ttaattcctt
     5641 gaacgaatat ttactgccat ttcattcacg tctattctta atttgctgct ttaaaaacaa
     5701 gtgattgagc aaaattgagg caaaaatgga ctggtatctg aaagtaataa aaaattatgt
     5761 cggtttccgg ggtcgtgcgc ggcgcaaaga gtactggatg tttattctgg tcaacatcat
     5821 ctttacgttc gtgctggggc tgctggataa aatgttaggc tggcaacgcg ctggtggcga
     5881 aggcatcctg acgacaatct acggtattct ggtgttttta ccgtggtggg cagttcagtt
     5941 ccgccgcctg cacgacaccg accgctcggc gtggtgggca ctgctattct taatcccgtt
     6001 tatcggctgg cttattatca tcgtctttaa ctgccaggcg ggtacgccag gcgaaaaccg
     6061 ctttggacct gatccaaaac tggaacctta aaacaaatct gctcgccgga tgtctgactc
     6121 atccggcttt tatcatcatg ttgatgaatg a
//
LOCUS       DAZPDU010000224         6135 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZPDU010000224 DAZPDU010000000
VERSION     DAZPDU010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6135
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.226"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..1255)
                     /locus_tag="ACHURS_004042"
     CDS             complement(<1..1255)
                     /locus_tag="ACHURS_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFR1239649.1"
                     /translation="MAQFKVDDFLIELSFNSQKVLKGLEKAEKQTMQVASRIEKRLNK
                     SFRVNPTPLNDSLKLMERNVDKTVSKIEQRLKNTRAFKIKTEIEDTLKPLRQPRQPRI
                     SGSRAITAAYSANMSKLRDFNPMLEKYFKSQYYSLSSKSKTLGNETFNRELAKLNQSL
                     RETLSKFNKTTSKNNHSENASNSLDVLATSAIKAGTAIYSFQTALEAYRRIMEVGLKK
                     EASQRAAQFVLGDEGAKRATEFVKNLADSSGVDQIETLSSFAKFSAGAGDMNADQKES
                     LFSNVIGTSRLMGLSTDEINGILKAFEQMASKGKIQAEELRGQLGDRMAGAFQLFARS
                     LGMTTEELDKAMKDGKILSKDVLPKVSAEMGRMIDKAGGWEKIINSTQTQLGRLSNSW
                     NNNLALMFDGSQEGLTDFTRSLTNLL"
     gene            complement(1412..1621)
                     /locus_tag="ACHURS_004043"
     CDS             complement(1412..1621)
                     /locus_tag="ACHURS_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239650.1"
                     /translation="MFEERLETIRSVCENLKLQTKPALRIKNKRQVITSHKPKTRKIP
                     KWCIDRIPSDAQIIGETELHYLVRH"
     gene            complement(1644..2354)
                     /locus_tag="ACHURS_004044"
     CDS             complement(1644..2354)
                     /locus_tag="ACHURS_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1239651.1"
                     /translation="MTNDLYINGKKVDSFGDWSPQGEHPLHTIQRQEHDKRIELEQEL
                     KMSPKVISFVSPEPQEMPDICKTEALFELEREYYPALKSQRLRLDVAYDAVKNFEETS
                     KPSEYDIAYEIKSNPFIYYEGNFNDGFGTAIEDVPKVLQSIPEGFRLIDVRQALRGSG
                     QFILMTDKTDEEILQLAEANLTKHYSGKLDKLKQKLKKELDTMKELVSEYESQKKIAL
                     MADIEQLTKISQKYSKAV"
     gene            complement(2870..3193)
                     /locus_tag="ACHURS_004045"
     CDS             complement(2870..3193)
                     /locus_tag="ACHURS_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239652.1"
                     /translation="MNEYELQIFIDSDTAMMVQAFADSGVSIDLDKLLKLMAENSEEI
                     EDFIQEIETGEPRFMFPVTDSNMKRLIIEETNKYSVSPEKYLKAAIAILYGDNILVTD
                     SKVVH"
     gene            complement(3372..3572)
                     /locus_tag="ACHURS_004046"
     CDS             complement(3372..3572)
                     /locus_tag="ACHURS_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239653.1"
                     /translation="MLCIKFEYLTDKMIKHVSDLLIKEGGFGDACNPKDIFIHATSPN
                     ATLKTAVTAEWFERNKAELGYW"
     gene            complement(3572..3844)
                     /locus_tag="ACHURS_004047"
     CDS             complement(3572..3844)
                     /locus_tag="ACHURS_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000816065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFR1239654.1"
                     /translation="MKQWRDDIKRFFATYFMINYETGMLEWIDGGEVKTRDDAYGNPM
                     IRYGTRDYYLKYVIWFLHHEVQATKKIIFRDGNKRNCHPNNLLLEI"
     gene            complement(3847..4125)
                     /locus_tag="ACHURS_004048"
     CDS             complement(3847..4125)
                     /locus_tag="ACHURS_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239655.1"
                     /translation="MNKHTLTRVTKTAFDELNRIKNITGLPHSAVMKLAINRDINEQD
                     LLNSRLEAARHHIRIKQDDLDSLKSLNAFGLDLARLLSIKIHKLSQEV"
     gene            complement(4126..4683)
                     /locus_tag="ACHURS_004049"
     CDS             complement(4126..4683)
                     /locus_tag="ACHURS_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239656.1"
                     /translation="MQYSEFRAMLQASRERNKHNSYAYTNNPTSYEVPTFSKSERKNI
                     EAVIRSITPRDRFMPVRKTTKNTIKNYLANFDSYEQLPSKLDDVFIGFCRSEGHPKYN
                     KKLFYLLKNLDEINASTVTNHLQRQATRLSYELPSDKYCALLAVMCAKLIGIVEHHIA
                     VGNIEPMENEQPDFEFDPYLVTEDF"
BASE COUNT         1747 a         1276 c         1069 g         2043 t
ORIGIN      
        1 taagtaaatt agtcagtgac cttgtaaagt ctgttaaacc ttcctgtgaa ccatcgaaca
       61 ttaaggccag attattattc catgaattac ttaagcggcc taactgcgtc tgagtagagt
      121 taataatctt ttcccatcca ccagctttat caatcatcct tcccatttca gcggatactt
      181 taggaagaac atctttagaa agaatcttac cgtctttcat ggccttgtct aattcttccg
      241 tagtcatccc aagacttctg gcaaataact gaaaagcacc tgccattctg tcacctaatt
      301 gaccgcgtag ctcttcagcc tgaatctttc ctttagatgc catctgctcg aaagctttta
      361 aaatcccgtt aatttcatcg gtgcttagcc ccattaatct ggatgtacca atcacattag
      421 aaaataagga ttctttctgg tctgcattca tatcaccagc accagcggaa aacttagcaa
      481 aactgcttaa tgtttcaatc tggtctacac cactactatc agcaagattc ttaacaaatt
      541 ctgtagctcg tttagcacct tcatcaccta aaacgaattg tgccgctcgt tgtgatgctt
      601 cttttttcag accaacctcc ataatccgcc tgtaagcttc taaggctgtc tgaaaactat
      661 aaatggctgt acctgctttg attgcactcg tagctaatac atccagacta tttgaagcat
      721 tttcactgtg attatttttg ctggtggtct tgttaaactt agaaagtgtt tcacgaagag
      781 actgatttaa ctttgctaac tcacgattaa aagtttcgtt tccaagtgtc ttggatttac
      841 tgcttaagct gtaatactgg cttttaaaat acttctcaag cataggatta aagtcacgaa
      901 gcttgctcat attcgctgag taagctgctg tgattgctct gcttccgcta attcgtggct
      961 gtcttggttg tctaagtggc tttaaagtat cctcaatttc agttttaatc ttaaacgctc
     1021 tagtgttttt aagacgctgc tcaatcttgg aaacagtttt atcgacattt ctttccatta
     1081 gtttgagaga atcatttaaa ggcgtaggat taactctaaa cgacttattc aatcgcttct
     1141 caattcgtga tgctacctgc atcgtttgtt tctcggcttt ttccaagcct tttaaaactt
     1201 tctgagagtt aaaacttaac tcaatcagaa aatcatcaac tttaaattgt gccattcata
     1261 tttttcacct ctttaagcgt gttgtacgta aattctattt ttcagtgtgg gtattagctt
     1321 tacagatttc acgtagcaca catacaaaaa aagggagagc taccgatgtc actcgataat
     1381 ctctccccgt atttacccaa ccaattcttt tttaatgtcg gacaagataa tgtaattccg
     1441 tttcacctat gatttgagca tcagaaggaa tacggtcaat acaccacttt ggaatcttgc
     1501 gtgtttttgg tttgtgtgag gtgataactt gacgtttgtt tttaattctt aaggctggct
     1561 tagtttgtaa ctttaagttt tcacaaacag accgaatagt ctctaaacgt tcttcaaaca
     1621 ttaatcacct ctttaattca taatcaaaca gccttgctat acttctgaga aatcttcgtt
     1681 aactgctcaa tatcagccat caaagcaatc ttcttctgcg actcatattc actaacaagt
     1741 tctttcatag tatcaagttc ttttttaagc ttttgcttta acttatccag tttaccggaa
     1801 taatgcttag tcaaattagc ttcagctaac tgaagaatct cttcatctgt tttatcagtc
     1861 atcagaataa attgaccaga accccttaat gcttgtctga catcaattaa ccggaaccct
     1921 tcagggatac tttgaagaac tttaggaacg tcttcaatcg ctgtaccaaa accatcatta
     1981 aagtttcctt cataatagat aaacgggttt gacttaatct cgtaagcaat atcatattct
     2041 gaaggtttcg aggtttcttc gaagttctta acagcatcgt aagcgacatc aagtctaaga
     2101 cgctgtgatt ttaaagctgg gtaatattca cgctcaagct caaatagtgc ttcagttttg
     2161 cagatatcag gcatttcttg aggctcagga gacacaaagg aaattacctt tggtgacatc
     2221 tttagctctt gctcaagctc aatgcgcttg tcatgctctt gacgctgaat agtatgtagc
     2281 ggatgttcac cctgtggtga ccagtctccg aagctatcaa cctttttacc gttaatatat
     2341 aaatcgtttg tcattataat ttcctctaag tgtggttatt tcttaataga gcaccttcaa
     2401 cgccaataca cttagaagat gacttaacga tttattaaag atgctctatt aagaaaagcc
     2461 ctaaacataa tctgtgaagg gctttggagg tttgcttata acgttaacga agtttcagga
     2521 aaactttctt taaaagctct gtttaaaaac tcttaaagaa agccctaact catgattagg
     2581 gcaaagctgt aaaggattca ataatgaatg aacaaagact ttttgagata gcctctcaga
     2641 ccaacctgaa aggctaggac gaaaagcatt gcacctttcc aactcagaat atccatccat
     2701 cggattattg aaagaaaaaa taagagagaa ttgctctccc ccatatatac aattatgtca
     2761 tgctcaaggt tgtttgtcaa cagcttttaa cagattggta acatttatac tttaatagat
     2821 gcacctgcaa tcataccaat caatacaaaa cagataatca ccaattccat taatgcacca
     2881 ccttcgaatc cgtcaccaga atattatcgc catacaaaat tgctatagcc gccttgagat
     2941 atttttcagg gctaacgctg tatttgttcg tttcttcgat aataagacgc ttcatgtttg
     3001 agtcggtaac aggaaacata aaacgcggtt caccagtttc aatttcctga ataaagtctt
     3061 caatttcttc tgagttttca gccattagct ttagtaattt atcaaggtca attgatacac
     3121 cggaatcagc gaaagcctgc accatcatag cagtgtcgct atcaatgaaa atctgtaatt
     3181 catattcgtt catttttaaa atctcctttt taccagacct cctcaatcgc tctgtaacga
     3241 gctaaacagt ctttttcagg ggtagcctgt acagtggtat tacccaccct aaaataagat
     3301 agttttttgc gttagaatgc gattgagagc gtctgataag ctgtttttct aatttctctt
     3361 aaagtctcgg actaccaata acctaattcc gctttatttc gttcaaacca ttccgctgta
     3421 actgctgtct ttagtgttgc gtttgggctg gtggcatgaa tgaagatatc ttttggattg
     3481 caagcatcac cgaaaccacc ttctttgatg agcaggtctg atacatgctt aatcatttta
     3541 tctgtcaaat attcgaattt aatacataac attaaatctc caggagtaaa ttgtttgggt
     3601 ggcaatttct tttattacca tccctgaaga taatcttttt agttgcctga acttcatgat
     3661 gcaaaaacca gatgacatac ttcagataat aatcacgagt gccataacga atcattggat
     3721 taccgtaagc atcatctctt gtcttaacct ctccaccatc aatccattca agcattcctg
     3781 tctcataatt aatcatgaag taagttgcaa agaaccgttt aatatcatct cgccattgct
     3841 tcataatcag acctcctgag acagtttgtg aattttgata cttagtaacc ttgctaaatc
     3901 cagtccaaaa gcatttaagg atttcagact atcaaggtca tcttgcttaa tcctgatatg
     3961 gtgtcttgca gcctctaagc gactattaag caaatcctgt tcgttaatat cacggttaat
     4021 tgcaagcttc atcactgctg agtgtggcag acctgtaatg tttttgattc ggttcagttc
     4081 atcaaaggct gtctttgtaa ctctggttaa tgtgtgttta ttcatttaaa aatcctctgt
     4141 aactaaatag gggtcaaact cgaaatctgg ttgctcgttt tccattggct caatattccc
     4201 aacggcaata tgatgttcaa caatgccaat taatttcgcg cacatcacag caagcaaagc
     4261 gcaatattta tcgcttggta attcgtaact aagtctggtt gcctgtcgct gaagatgatt
     4321 tgtcacggtg gatgcgttaa tttcgtcaag attctttagc agataaaata gttttttgtt
     4381 gtatttcggg tggccttcac ttctacagaa cccaataaaa acatcatcaa gcttgcttgg
     4441 taactgctcg taactgtcaa agttagccag ataattttta atagtattct ttgttgtttt
     4501 gcgtactggc ataaatctgt ctcgtggcgt aatgctacga atgaccgctt caatgttctt
     4561 acgttcggat ttgctaaagg ttgggacttc gtagctggtg gggttgtttg tgtaagcgta
     4621 ggagttgtgc ttgttgcgtt ctcttgatgc ctgaagcatc gctctaaatt ctgaatactg
     4681 cattcatgtt ctgactctca taagatgtta ttattctgtt aacctatata tacaatatta
     4741 gcattttcaa agactaatgt caagtatttt taacattttc gtaacatctg tggtataatg
     4801 ggtttatggg gagagtaaaa tatgaggcgt ggctatgtat ttggtcgcat attttgcatt
     4861 gcacacagac ctcttcaatc gccctgtaac gagttaaacg acctcttcag gtcaactcat
     4921 aaggtcaggt actttttgtg tcagaatgcg attgagagcg tcttacaagg aaaactaatg
     4981 gtaaatctcc ctcctgagct ggggtcacct gatgtatgga ctggtggagg tattgggctg
     5041 attgcaggcc tgctacttcg ctacgtcctc gtcagagtgc ttgttgtctt taaactgttc
     5101 ggtcgtacat ggacgcttga ccttaaggat aacgaccaca cagacccccg ttaaactcct
     5161 tcaaaacccc tgcacgagat gacagataga agtctatggt acaggggtgt ctttagcatt
     5221 ttgtgctatc tattaaaaaa tagcttgaca gaaccggaaa gctgtatcta tcaagcattc
     5281 acctctctga cagtaatttc taaaagttct tatttttcaa taagttgtaa ttagccatat
     5341 atagagaact gacaagtctc ttcacagaca actctgcaac cttaacagta acttctaaaa
     5401 atgcttattt ttcaacgagt tgtaatcacc caatatataa ggctgtccag taccttcaca
     5461 gataacttca agccctctta accgccccac taatcgggag aagagcgata accccagaga
     5521 gggaagctcc tttcttaacc gacctcttaa acgaccttta cagaccttgt tcaaggtaat
     5581 cacaatagtt tttcttccct cggtttcctg tgttcctcgg ctggctattg agatttgaaa
     5641 gacctctgta agcgctctct tagcgagaga aaaatcattc atagtgaccc caagcgaagc
     5701 gcccctctaa aaaaagattt tttagctatt ttgtttaaaa gcatatcaaa cgatacattt
     5761 tattgattta ggtcaaagac aaaagcattg acaaaatggt aagattgttc acatgacctt
     5821 acttatttaa gagactttga aacagcctgt ttaacaggtc actacctctg gtcttatcgt
     5881 tcccttcttt ctaggtggtt atctgttaag agtttttcag tttttattct taagataatt
     5941 gttaagatga tactctccct tcgggttata tcgtatctct tctcccgtta aatatccctt
     6001 cacagtcaga ctaaataaca cctgtgtagt tcttggtaga atctttcaag gtaacttagc
     6061 tctgagttta tgaagttgac ctatgagata gactctaccg aatctgttag tcaattgttg
     6121 cttaggtgct gctaa
//
LOCUS       DAZPDU010000225         6121 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZPDU010000225 DAZPDU010000000
VERSION     DAZPDU010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6121
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.227"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..358
                     /locus_tag="ACHURS_004050"
     CDS             <1..358
                     /locus_tag="ACHURS_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR1239657.1"
                     /translation="PGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPA
                     YTSQRCACCGHTAKENRLSQSKFRCQVCGYTVNADVNGARNILAAGHAVLACGEMVQS
                     GRPLKQEPTEMIQATA"
     gene            complement(398..1069)
                     /locus_tag="ACHURS_004051"
     CDS             complement(398..1069)
                     /locus_tag="ACHURS_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFR1239658.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPFFIYRADGQPIFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPVC"
     gene            complement(1176..1409)
                     /gene="yedF"
                     /locus_tag="ACHURS_004052"
     CDS             complement(1176..1409)
                     /gene="yedF"
                     /locus_tag="ACHURS_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFR1239659.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            complement(1406..2611)
                     /gene="yedE"
                     /locus_tag="ACHURS_004053"
     CDS             complement(1406..2611)
                     /gene="yedE"
                     /locus_tag="ACHURS_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFR1239660.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            2798..3211
                     /gene="yedD"
                     /locus_tag="ACHURS_004054"
     CDS             2798..3211
                     /gene="yedD"
                     /locus_tag="ACHURS_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFR1239661.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            complement(3245..4732)
                     /gene="amyA"
                     /locus_tag="ACHURS_004055"
     CDS             complement(3245..4732)
                     /gene="amyA"
                     /locus_tag="ACHURS_004055"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFR1239662.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(4810..5175)
                     /gene="fliT"
                     /locus_tag="ACHURS_004056"
     CDS             complement(4810..5175)
                     /gene="fliT"
                     /locus_tag="ACHURS_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFR1239663.1"
                     /translation="MNHAPHLYFAWQQLVDKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            complement(5175..5585)
                     /gene="fliS"
                     /locus_tag="ACHURS_004057"
     CDS             complement(5175..5585)
                     /gene="fliS"
                     /locus_tag="ACHURS_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFR1239664.1"
                     /translation="MYTASGSKAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            complement(5600..>6121)
                     /gene="fliD"
                     /locus_tag="ACHURS_004058"
     CDS             complement(5600..>6121)
                     /gene="fliD"
                     /locus_tag="ACHURS_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFR1239665.1"
                     /translation="YKEVDPGAEAQDKNNGALLGDSVVRTIQSGIRAQFANGASDSAF
                     KTLNEIGIKQDGTTGKLKIDDDKLKKVLNENTASVRELLVGDGKETGITTKIATEVKG
                     YLADDGIIDSAQDSINATLKKLTKQYLSVSASIDDTVARYTAQFTQLDTMMSKLNNTS
                     TYLSQQFTAMSNS"
BASE COUNT         1497 a         1609 c         1527 g         1488 t
ORIGIN      
        1 gccggggcgc aatgtccggg caaaatcagg tttaaaccgt tcgatactgg atcagggctg
       61 gtatgaaatg cgccgccagc ttgagtacaa gcagctctgg cgtggcggtc aggtgcttgc
      121 tgttccgcca gcgtacacaa gccagcgttg cgcgtgctgt ggtcatacag cgaaagaaaa
      181 ccgcctgtca caaagtaaat tcagatgcca ggtatgtgga tatacagtga acgccgatgt
      241 aaatggcgct cgtaacattt tagcggcggg gcacgccgtt cttgcctgtg gagagatggt
      301 gcagtcaggc cgcccgttga agcaggaacc caccgaaatg attcaggcga cagcctgaac
      361 gtagcaggga tcctcgtcct tcagggcgtg gaggatgtca acaaacaggt tgaattaact
      421 ccgcgccctg gtttttgaca ttacctaccg cgcgcgatac cgggtgccag gtgaactggt
      481 ttgctggcac acagccgttg gtagcaattt ctgatgcttc tttaccgcca atctcttgcc
      541 gcatccattc ctgtgcagct tctggcgaca gaaccagcgg gcggcggtca tgaatgtcta
      601 ccagaccctg gtctgctgca gcagtgacaa ttaaaaatcc ttcggcttcg tcaccacgct
      661 caaatggtgt gctacctatc gcggccataa aaataggttg tccatcagca cgatagataa
      721 aaaaaggctg ttttttgtcg ccttcttttt tccactcaaa ccagccatcg gcaaaacaga
      781 ttgcccgacc atgttgccag agcggtttaa acatgcgact ggttgcggca gtttctacgc
      841 gggcgttaat cagcggcggt ttatcccacc atccgggagc atatccccag aaaaccggat
      901 ccagatgaag gtgttcatca cgttcactga gcagaagaac tttggttccc ggcgcgacgt
      961 tgtatctgcc aatgggttcg ggatcgtagg gaatatcgcg ttcaatatct tccgcgagaa
     1021 gcgcaaggta atcttcacgc gtttgggatt gggcaaagcg tccacacata gaaacctcct
     1081 gagatcatac tgaaaagaat aggagagggg gcgggtaaaa tctatgtgtc agaggcaaca
     1141 atgttcccgc tgtgggccac agcgggaatg agggattact tctgaattaa ataacgaatg
     1201 gtcggcccgt cttgctgaat atccagcacc gtatagccgt gattacgcgc atccagtgga
     1261 atattattga tcgactgtgg acagtcgctc accacttcca ggatttcccc tttttttaac
     1321 tgcggcatcg cctcaagggt tgcgactgcc ggataagggc agggttcacc caccatatca
     1381 aggcggtaat caggaacgat atttttcatg cgatctcctt agcagtctgc ggtgccgcac
     1441 ggcggaagaa gcgtttttcc cagccgataa tcaacatcag cgcagtaaac aacagcaaat
     1501 atgtcaccag caggccaccc atcgggccaa aggttttcag caggttgatt ttgtcccagt
     1561 cggtggccag cgccggagcg aaatcatccc agtaatacgc cagaatcgtc gagccgatca
     1621 cattgcccag accgacccac cagtagtgca cctggccttc taccgcgcgg tacatccagc
     1681 cggtttcgca gccgcctgcc agcacgatgc caaaaccaaa cagtaaacca ccaattaccg
     1741 cgtttggtcc cgcccacatg attttgggtt caacgcctaa ctgtacgtaa ctgaagatcc
     1801 cgatggcact caccgccata ccgataatga ttgctttcgc catatgggta cgtccggtga
     1861 tccacatatc gcggaacgcc gaagtaaagc agatttgcgc acgttcaatc agtaaaccaa
     1921 agccgacgcc aaacagcatt gccagcccca gtttgggttg gttcatcgcc gtgagcagcg
     1981 cccagcccag catgccgaaa aagaccagca tcccgagacg aaaacgacgc cgcgcctgat
     2041 ccggtttttg cgtcagcggt gaggcggcag aaactttctg cattttcacg ggaatacgga
     2101 agatgggcag cagggtaaag cgcgcgccaa accatgaacc aatggcagtg gcgatggcaa
     2161 agaaccaggc atgcagcgag aactgaggaa tgccggtaaa gaacgccgcc aggttacagc
     2221 ccattgccag acgcgcgcca aaaccggcga taatgccgcc aataatggcc tgcataatgc
     2281 ggatacggct gcgcggcatg cgcagtttga cattgttggc ccacagcgct gcggcaaagc
     2341 agccgccaaa catgccgagg atcatcatcc cgtcgatgcg ggttaatggc gatccttcca
     2401 gatggataat tttaaaataa ccccactctt cagcatggac gccaaacagc tgcaggagct
     2461 gaccgcccca acgggtaaat tcacccgtga cagcccaaaa ggtgccagta atgccaaaat
     2521 agtaagtgga gagaataccc gccgcgatga ccgcagggat gggtgcccag aatttaatca
     2581 accaggcgtg tttgaattgc tgccatgaca tgaatgtagc ctctgaactc agaaggtttg
     2641 aaacaagaat cgcgaatcat acatcatgca aatttatttt cggggtaata attcacaaaa
     2701 ataatagtta gatcaaattt ctctctgaac ctggaaaggc tggcgacatc agggctttaa
     2761 tgccacaatc ctcttttctt ctcatgcagg acgaatgatg aaaaaactcg caattgcagg
     2821 tgcgctcatg ttgctggcgg gctgtgccga agtcgaaaac tataacaatg tcgtgaaaac
     2881 ccccgcgccg gactggctgg cgggctactg gcagaccaaa gggccgcaac gtgcgctggt
     2941 cagtccggaa gcgataggca gcctgattgt cactaaagag ggcgataccc tggattgccg
     3001 tcagtggcag cgggtgattg ccgtgccggg caagttgaca ttgatgtctg atgatctcac
     3061 taacgttacc gtaaaacgtg agctgtacga ggttgagcgt gacggtaaca ccattgagta
     3121 cgatggtatg acgatggagc gtgtggatcg cccgacggca gagtgtgccg ccgcgctgga
     3181 taaagcgccg ttaccgacgc cactgccgta atgaaatctc cccggcttgc cgccggggat
     3241 gaatttaaat cacctcttcg ataacccaca cgctgacgct gccgccgttg caaaagaagg
     3301 tcgcttcgcc gttttcgtcg gtcactacgc tctcttgccg attccccaga aaatcacgcc
     3361 aggttttatt gccgtaattc tctcccagat gaatggtttt ttcgccatca tccccgttcg
     3421 acatgaccac cacgcagccg ggatattcgt cagtgccact gcggctaaag gcaatgcagt
     3481 tcggatggtc gaaaaataac gtctgtacac cgtgggcgaa acgctgacgg gcgagaatta
     3541 actcatcaag ctgttcgatt attggcatat ctatcggata ggtttgcccg tcaccaccga
     3601 catcttcgta atgcgcaccg tagaggtccg gatagaacac cgaaggaacg ccattttccc
     3661 gcaacaaaat tagagcatac gccagcggtt taaaccacgg ttcgaccggc gcttcgaggg
     3721 cttgcaacgg ttgggtgtcg tgattggcta cgagggttac ggcgtggaaa ggatcggctt
     3781 ccactaatgt acccgtgaaa atctgcgtca tgtcgtagtc gcgtcccatg cgcgatgctt
     3841 catggaattt catctgcagc ggcgcatcga acagcatggt tttgccttcc acctgatcaa
     3901 tatacgtttg cagtttatcg acttcatgcg accagtactc tgccacaata aacagcggct
     3961 ttggcgcaac ttcctgtacg tgttcgatcc actctttata aaaccacgcc ggaatatgtt
     4021 taaccgcatc aagacgaaaa ccgtcgcatt gtgtttgttc catcacccag cgcgcccagt
     4081 atttaatctc ttccgtcacg gcatgattgc ggaaatcgat attctcaccc atcagataat
     4141 cgaaattacc gagttcatca tcaacctgat cgttccagcc ttcgccggtg tagtcgttaa
     4201 caattttaaa tatgccatct tcatcaggat tttcgatatg gtcgataccg ctaaaacatt
     4261 tgaagtccca gataaactgc gagtattgcc cggcacgggc ggggaaggtg taacgcgtcc
     4321 agccttcgca ctcaatgatt tcttcatcaa tttgcgtacg gtcatcagca tttacacgct
     4381 gcacgcgaat agcttctttt tcatccgccc ccattttgtg gttgactacc acgtccagta
     4441 gcaccgcaat gtcattacgt ttcagagcgt caatggcggc cagcagttgt gctttatcgc
     4501 catatttagt agggatgctg cctttctgat caaactcacc taaatcaaat aaatcatagg
     4561 agtcgtagcc gaccgaatac ccgcccgatg cgcctttata ggcgggcggc aaccagacca
     4621 tattgatacc aatatcatta aaaccgtcgg cgcgctcggc cagttcaggc cagagcttac
     4681 cgccttccgg gtaataccag tgaaaacatt gtaacagcgt gggattacgc attcttcgac
     4741 tccattcaag gggaacatta gaagcgtagc cgtaatcgga ttattcgcga gccatcggct
     4801 cgttaagatt caaaagaggt tatcctgcgg agccagcaca aagccgccct gatctccata
     4861 ggcacttaac actgattttt gcaccgatga ctgaccgacc agtttcgcca gttcatccat
     4921 acgaatctgt aataactgct ttaccttgct ttcgttgtcg agaatcaggc gcagcatcgg
     4981 gcggagctgc tcctgcatcg tggtggacgg ggcaacctct tcagtcagat gtgcaatctc
     5041 ctgcaccgca ttcacatacg ccatttcgct ggcgatgagt tcgtcccatt gttcttccgt
     5101 tgccaggcgt aacatgagct ggcttttatc gacgagttgt tgccaggcga aatacaaatg
     5161 cggtgcatgg ttcatcagac tgggtcctga atcaaagaag gggagagtaa agactctttc
     5221 caggcatcgg caatattgcg cattaatgct tccacttctt cgactgcgga gacatcgttg
     5281 cgtaaattgg cttgcagcaa gcgcctgacc atatagctat aaagagcaat caagttttgg
     5341 gttagttcgt ctttgctctc ttcatcaaga ctcacccgca gcccgttctc aatgatgttg
     5401 atcgcttttg acaaagagac gcctttgcct tgctgattgt tgtcctgcat aaacaggcgc
     5461 gctctaacca gtgcgctcag cactccatca aatagcatgg tgaccagctg ttgctggctg
     5521 gcgctcatta cggcgctttc gacgccaatt tgtgcatagg cttttgagcc gctcgcggta
     5581 tacatgctga cctccgtgat caggaattac tcattgcagt aaattgttgg ctgagatagg
     5641 tactggtgtt attcagtttg ctcatcatgg tatctaattg cgtaaactgt gcagtgtaac
     5701 gcgccacggt gtcatcaatg ctggcactga cggatagata ctgtttggtc agttttttca
     5761 gtgtggcgtt aatactgtcc tgtgcgctat caataatgcc atcatcggca aggtaacctt
     5821 ttacctcggt ggcaattttg gtggtaattc ctgtttcttt accatcgcct accagcaatt
     5881 cacgtacgga tgctgtattt tcattcagga cttttttgag tttatcatca tcaattttca
     5941 gcttacccgt cgtgccgtcc tgcttaatcc cgatctcgtt taatgttttg aaggcactgt
     6001 cgctggcacc gtttgcaaat tgtgcgcgga tgccgctttg aattgtacgt acaacgctgt
     6061 ctccgagaag tgcaccatta tttttatctt gtgcttcagc acctgggtct acttctttat
     6121 a
//
LOCUS       DAZPDU010000226         6098 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZPDU010000226 DAZPDU010000000
VERSION     DAZPDU010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6098
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.228"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            235..1341
                     /gene="ompC"
                     /locus_tag="ACHURS_004059"
     CDS             235..1341
                     /gene="ompC"
                     /locus_tag="ACHURS_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFR1239666.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKSEDGDQTYVRLGFKGVTQVTDQLTGYGQWEYQIQGNTSEDNKENSWTRVAFAGL
                     KFQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFG
                     LVDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGGAISSS
                     KRTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQN
                     FEAVAQYQFDFGLRPSVAYLQSKGKNLGTIAGRNYDDEDILKYVDVGATYYFNKNMST
                     YVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1453..2508
                     /gene="apbE"
                     /locus_tag="ACHURS_004060"
     CDS             1453..2508
                     /gene="apbE"
                     /locus_tag="ACHURS_004060"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFR1239667.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGTKTDGAMDITVGPLVNLWGFGPEQQSVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLNVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLISEKN"
     gene            2582..3646
                     /gene="ada"
                     /locus_tag="ACHURS_004061"
     CDS             2582..3646
                     /gene="ada"
                     /locus_tag="ACHURS_004061"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFR1239668.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3646..4296
                     /gene="alkB"
                     /locus_tag="ACHURS_004062"
     CDS             3646..4296
                     /gene="alkB"
                     /locus_tag="ACHURS_004062"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFR1239669.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTGDCRYNLTFRQAGKKE"
     gene            4372..6015
                     /gene="yojI"
                     /locus_tag="ACHURS_004063"
     CDS             4372..6015
                     /gene="yojI"
                     /locus_tag="ACHURS_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFR1239670.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAITLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         1450 a         1539 c         1646 g         1463 t
ORIGIN      
        1 tgatgttagg tgcttatttc gccattccgc aataatctta aaaagttccc ttgcatttac
       61 attttgaaac atctatagcg ataaatgaaa catcttaaaa gttttagtat catatttgtg
      121 ttggattatt ctgcattttt ggggagaatg gacttgccga ctgattaatg agggttaatc
      181 agtatgcagt ggcataaaaa agcaaataaa ggcatataac agagggttaa taacatgaaa
      241 gttaaagtac tgtccctcct ggtcccagct ctgctggtag caggcgcagc aaacgctgct
      301 gaagtttaca acaaagacgg caacaaatta gatctgtacg gtaaagtaga cggcctgcac
      361 tatttctctg acaacaagtc agaagacggc gaccagacct atgtacgtct tggtttcaaa
      421 ggcgtaactc aggttactga ccagctgacc ggttacggcc agtgggaata tcagatccag
      481 ggcaatacct ctgaagacaa caaagaaaac tcctggaccc gtgtggcatt cgcaggtctg
      541 aaattccagg atgtgggttc tttcgactac ggtcgtaact acggcgttgt ttacgacgta
      601 acttcttgga ccgacgtact gccagaattc ggtggcgaca cctacggttc tgacaacttc
      661 atgcagcagc gtggtaacgg cttcgcgacc taccgtaaca ctgacttctt cggtctggtt
      721 gacggcctga actttgctgt tcagtaccag ggtaaaaacg gtagcgtaag cggcgaaggc
      781 atgaccaaca acggtcgtgg tgctctgcgt cagaacggcg acggcgttgg cggatctatc
      841 acttatgatt acgaaggctt cggtatcggt ggtgcgatct ccagctccaa acgtactgat
      901 gatcagaaca gcccgctgta catcggtaac ggcgaccgtg ctgaaaccta caccggtggt
      961 ctgaaatacg acgctaacaa catctacctg gctgctcagt acacccagac ctataacgca
     1021 actcgcgtag gttccctggg ttgggcgaac aaagcacaga actttgaagc tgttgctcag
     1081 taccagttcg acttcggtct gcgtccgtct gtagcatacc tgcagtctaa aggtaaaaac
     1141 ctgggtacca tcgctggtcg taactacgac gacgaagata tcctgaaata tgttgatgtt
     1201 ggtgctacct actacttcaa caaaaacatg tccacctacg ttgactacaa aatcaacctg
     1261 ctggacgaca accagttcac tcgtgacgct ggcatcaaca ctgataacat cgtagctctg
     1321 ggtctggttt accagttcta atctcgattg atatcgaaca aagggcctgc gggccctttt
     1381 ttcattgttt tcagcgtaca aactcatttt tttggtgtac tcttgcgacc gttcgcatga
     1441 ggataatcac gtatggaaat aagctttacc cgcgtggcac tgctggctgc cgcgctcttc
     1501 tttgttggtt gcgatcaaaa accacaaccc gccaaaaccc acgctactga agttaccgtt
     1561 ctagaaggca aaaccatggg taccttctgg cgtgccagca tcccgggcat tgatgccaaa
     1621 cgcagtgccg aacttaaaga aaagattcag acccagctgg acgccgacga tcagctgctt
     1681 tcgacctata aaaaagattc cgcgctgatg cgctttaacg attcgcaaag tttatcgccg
     1741 tggccggtaa gtgaagcgat ggccgatatc gtcaccacct cgctgcgcat tggcacgaag
     1801 accgatggcg cgatggatat aaccgtcggg ccgctggtga atctgtgggg ctttggcccg
     1861 gaacaacagt cggttcaaat tccgagccag gaacagatcg atgcgatgaa agccaaaacc
     1921 ggcttacagc acctgacggt cattaatcag tcgcatcagc aatatctgca aaaagacctg
     1981 ccggatttaa atgtcgatct ctccaccgtc ggtgaaggtt atgcggcgga tcacctggca
     2041 cgcttgatgg agcaggaagg gatttcccgc tatctggtgt cggtgggcgg cgcgctgaac
     2101 agccgtggta tgaacggtga aggcctgccg tggcgggtag cgattcaaaa accaaccgat
     2161 aaagaaaacg cggttcaggc tgtggtggat atcaacggtc atgggattag cacctctggc
     2221 agctaccgta actattacga actggacggc aaacgtcttt cccatgttat cgatccgcaa
     2281 accgggcgtc ccatcgaaca caatctggta tccgtgacgg tgattgctcc gacggcgctg
     2341 gaagccgatg cctgggatac tggcttgatg gtactcgggc cggagaaagc caaagaggtt
     2401 gttcgccggg aagggctggc ggtttatatg atcaccaaag aaggcgatag ctttaaaacc
     2461 tggatgtcac cacagtttaa aagcttcctt atcagcgaaa aaaattaaag cgcaagattg
     2521 ttggtttttg cgtgatggtg accgggcaga ctaaaggcta tccttaacca gggagctgat
     2581 tatgaaaaac gccacatgct taactgacga tcaacgctgg caatctgtct tagcccgcga
     2641 cccgaatgcc gacggcgaat tcgttttcgc cgtgcgcacc acgggtatct tttgccgtcc
     2701 gtcttgccgc gccagacatg ccttgcggga aaatgtctcc ttctacgcaa atgccagcga
     2761 ggcactcgcc gccggatttc gcccctgcaa acgttgtcag ccagacaaag ccaatccccg
     2821 gcaacatcgg ttggataaaa tcacccacgc gtgtcgactg ctggaacagg aaacgcctgt
     2881 aacgctggaa gccttagccg accaggtggc gatgagtcca tttcatctac atcggttgtt
     2941 taaagcgact accggaatga cgcctaaagc ctggcaacag gcctggcgcg ctcgccgttt
     3001 gcgcgaatcg ctggcgaaag gggagagcgt gacgacgtct attcttaacg ccggattccc
     3061 cgacagcagc agttactacc gcaaagccga tgaaacgctg ggcatgacgg ctaaacaatt
     3121 ccgtcacggt ggcgaaaatc tggcggtgcg ttacgcgctg gctgattgtg agctgggtcg
     3181 ttgcctggtg gcagaaagcg agcgggggat ttgcgcgata ttgctgggcg atgatgacgc
     3241 gacattaatc agcgagttgc agcagatgtt tcccgctgcc gacaacgcgc ctgccgatct
     3301 gacgtttcag caacatgtgc gtgaagtgat cgccagcctc aatcaacgcg atacgccgct
     3361 gacgttaccg ctggacattc gcggcactgc ttttcagcaa caagtctggc aggcactgcg
     3421 cacgatacct tgtggtgaaa ccgtcagtta tcagcaactg gctaacgcca tcggcaaacc
     3481 gaaagcggta cgggccgttg ccagcgcctg tgccgccaac aagctggcta tcgtaatacc
     3541 ctgtcatcga gtggtccgtg gtgatggcac actttccggt taccgctggg gcgtgtcgcg
     3601 taaagcgcaa ctgctgcgcc gcgaagctga aaatgaggag aggtaatgtt ggatctgttt
     3661 gccgatgctg aaccgtggca agagccactg gcggctggtg cggtaatttt acggcgtttt
     3721 gcttttaacg ctgcggagca actgatccgc gatattaatg acgttgccag ccagtcgccg
     3781 tttcgccaga tggtcacccc cgggggatat accatgtcgg tggcgatgac caactgtggg
     3841 catctgggct ggacgaccca tcggcaaggt tatctctatt cgcccattga tccgcaaaca
     3901 aataaaccgt ggcccgccat gccacaaagc tttcatgatt tatgtcaacg tgcggctacg
     3961 gcggcgggct atccagattt ccagccagat gcttgtctta tcaaccgcta cgctcctggc
     4021 gcgaaactgt cgctgcatca ggataaagac gaaccggatc tgcgcgcgcc gattgtttct
     4081 gtttctcttg gcttacccgc gatttttcaa tttggtggcc tgaaacgtaa tgatccgctc
     4141 aaacgtttgt tgttggaaca tggcgatgtg gtggtatggg gcggtgaatc gcggctgttt
     4201 tatcacggca ttcaaccgct gaaagcgggg tttcatccac tcaccggcga ctgccgttac
     4261 aacctgacat tccgtcaggc aggtaaaaaa gaataaaaat aagaattatt attgctgtgc
     4321 gcgcgaagat gtttaaactg cgggctgtaa ttcattgtcc gggttttctg catggaactt
     4381 cttgtacttg tctggcggca gtatcgctgg ccatttatca gtgtgatggc gctaagcctc
     4441 gccagtgcgg ctttaggcat tggcttaatt gcttttatca atcagcgcct tatcgaaacg
     4501 gcggatacca gtctgctggt gttgccggag tttctgggat tattgctgct gttgatggca
     4561 atcactctcg gatcgcaact ggcgctcacc actttggggc atcacttcgt ttaccgactg
     4621 cgtagtgaat ttatcaagcg gattctcgat acccacgtcg agcgcattga acaactcggt
     4681 agcgcctcgt tgctggcggg attaaccagc gatgtgcgca atatcaccat tgcttttgtg
     4741 cgtctgccgg aactggtgca ggggatcatt ctcactatcg gttcagcggc gtatctgtgg
     4801 atgctgtcgg gcaaaatgtt gctggtaacg gctatctgga tggcggtcac catctggggc
     4861 ggttttgtgc tggtggcgcg ggtgtacaaa catatggcga ccctgcgtga aaccgaagat
     4921 aagctgtaca cggattttca aaccgtactc gaagggcgca aagagctgac tctgaaccgt
     4981 gaacgcgccg agtatgtgtt taacaacctc tacattcctg atgcgcaaga gtatcgccat
     5041 catattatcc gcgcagacac cttccatctt agtgccgtga actggtcaaa catcatgatg
     5101 ctgggcgcta tcggcctggt gttctggatg gcgaacagcc tcggttgggc tgataccaac
     5161 gttgctgcga cctattcgtt gacgctgtta ttcctgcgta cgccgctgct ttcggcggtt
     5221 ggcgcattgc cgacgctgct gacggcgcag gtggcgttta acaagctgaa caaattcgcg
     5281 ctcgcgcctt tcaaagcaga gtttccgcgc ccgcaggcgt ttcccaactg gcaaacgctg
     5341 gagctgcgta atgtgacgtt tagctatcag gataacgcgt tttccgttgg gccgattaac
     5401 ctcaccatta aacgtggcga gctgctgttt ctgattggtg gcaacggtag tggaaaatcg
     5461 acgctggcga tgttgctgac gggcttgtac cagccacaaa gcggcgaaat cttgctggat
     5521 ggcaaacctg tcagcggcga acaaccggaa gattatcgca aactgttttc ggcagtgttt
     5581 accgatgtct ggctgtttga tcaactgctg gggccggagg ggcaacccgc taacccgcaa
     5641 ctggttgaga agtggctggc gcagctgaaa atggctcata agcttgagtt aagcaacggg
     5701 cgtattgtta acctgaagtt atcaaaaggg cagaaaaaac gcgtggcgct gttgctggcg
     5761 ctggcagaag aacgcgatat tatcctgctg gatgaatggg cggcggatca ggatccacac
     5821 ttccgtcgtg agttttatca ggtgttgctg ccgctgatgc aggagatggg taaaactatt
     5881 ttcgccatca gtcatgatga tcattacttt atccacgccg accgcctgct ggaaatgcgc
     5941 aatgggcaac ttagcgagct gacgggcgaa gagcgcgatg ccgcttcgcg tgatgccgtt
     6001 gcccggacgg cataatatta cgacgctgac ctggcggact gctgccgcca ggtcaatatt
     6061 ctaaaaatcc cttcatttct aataacccta taattaat
//
LOCUS       DAZPDU010000227         6025 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZPDU010000227 DAZPDU010000000
VERSION     DAZPDU010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6025
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.230"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(127..276)
                     /locus_tag="ACHURS_004064"
     CDS             complement(127..276)
                     /locus_tag="ACHURS_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239671.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            847..1005
                     /locus_tag="ACHURS_004065"
     CDS             847..1005
                     /locus_tag="ACHURS_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR1239672.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDR
                     LRGLEVEA"
     gene            1465..2280
                     /gene="sul2"
                     /locus_tag="ACHURS_004066"
     CDS             1465..2280
                     /gene="sul2"
                     /locus_tag="ACHURS_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HFR1239673.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            2367..2669
                     /locus_tag="ACHURS_004067"
     CDS             2367..2669
                     /locus_tag="ACHURS_004067"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239674.1"
                     /translation="MVRKYFGTDGIRGKANEGAMTAETALRVGMAAGRVFRRGDHRHR
                     VVIGKDTRLSGYMLEPALTAGFTSMGMDVFLFGPLPTTYRKNKRPFHPSPTALDRS"
     gene            complement(2845..4338)
                     /locus_tag="ACHURS_004068"
     CDS             complement(2845..4338)
                     /locus_tag="ACHURS_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISVsa3 family transposase"
                     /protein_id="HFR1239675.1"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMA
                     EQGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARR
                     MAESAALLVDEVLPEQPMRQWVLSFPFQLRFLFASRPEIMGWVLGIVYRVIATHLVKK
                     AGHTHQVAKTGAVTLIQRFGSALNLNVHFHMLFLDGVYVEQSHGSARFRWVKAPTSPE
                     LTQLTHTIAHRVGRYLERQGLLERDVENSYLASDAVDDDPMTPLLGHSITYRIAVGSQ
                     AGRKVFTLQTLPTSGDPFGDGIGKVAGSSLHAGVAARADERKKLERLCRYISRPAVSE
                     KRLSLTRGGNVRYQLKTPYRDGTTHVIFEPLDFIARLAALVPKPRVNLTRFHGVFAPN
                     SRHRALVTPAKRGRGNKVRVADEPATPAQRRASMTWAQRLKRVFNIDIETCSGCGGAM
                     KVIACIEDPIVIKQILDHLKHKAETSGTRALPESRAPPAELLLGLFD"
     gene            complement(4382..4771)
                     /locus_tag="ACHURS_004069"
     CDS             complement(4382..4771)
                     /locus_tag="ACHURS_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239676.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4989..5429
                     /locus_tag="ACHURS_004070"
     CDS             4989..5429
                     /locus_tag="ACHURS_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HFR1239677.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
BASE COUNT         1428 a         1663 c         1581 g         1353 t
ORIGIN      
        1 aaaaaaaagg aatatttcgt tttcacgagt aatacccggg tattaaaaat ccactgtaat
       61 tacatgaaaa taaagaaata aaaggtaata ccggagtatt accacgtacg cgggcaagcg
      121 aaaaagctat aacgcttccc ggccgacagg cgacccaaaa tccacagggg catgcaggtt
      181 ctcttcagta acctgcgcca ttaaatcaaa aaggtcatat tcgccgggaa gctgcgtatc
      241 agtcgaatcc agttgttcat catattcaga tggcatgggg tgatatccag tagctgagag
      301 gttgttagcg ggtttcgggg cgcagccctg aaccagtcat gtagcgctag cggagtgtat
      361 actggcttac tatgttgcca cgattgcgaa aatcagtgaa gtgcttcatg tggcaggaga
      421 aaaaaggcag caccggcgcg tcagcagaat atgcgataca ggagatattc cgcttcctcg
      481 ctcactgact cgctacgctc ggtcgttcgg ctgcggcgag cggtaccggc ttacggacgg
      541 ggacggaaat ttcctggaag ataccaggaa gacacttagc aaagaagaga taaggccacg
      601 gcgaagccgt ttttccatag gctccgcccc cctgacaagc atcgcaaaat ctgacgctca
      661 aatcagtggt ggcgaaaccc gacaggacta taaagatccc aggcgtttcc ccctggtagc
      721 tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgtcattcc gctgttatgg
      781 ccgcggtttc tcattccacg cctgacactc agttccgtga aggcagttcg ctccaagctg
      841 gactgtatgc acgaaccccc cgttcagtcc gaccgctgcg ccttgtccgg taactatcaa
      901 cttgagtcca acccggaaag acacgacaaa acgccactgg cagcagccat tagtaacagg
      961 agtgcacgag aagatagatt aagaggtctt gaagtagagg cctgactgcg gctacactga
     1021 aagaacagtt ttggtaatgg cggtctcgtg aaccctgtta ccgggttaag cagttcccca
     1081 actgacttaa ccttcgaaca aaccgcctcc ccaggcggtt ttttcgttta cagagcaaga
     1141 gattacgatt cattgaaaag gatctcaaga agatcctttg tagattacta tcaattaatc
     1201 ttccgaacat gacaccagtc actgttgatt acggttaaac cgctccgtaa caggtttccc
     1261 tcgtattggt gacttgcagc accaaagaag caaaaaagat aaatatatct acttattgag
     1321 agatgatata tttatttcca aaatgctaca gatacatcat catgaaacgg atcaccgcaa
     1381 acaggttact cgcctgggga ttccctttcg acccgagcat ccgtatgaga ctcatgctcg
     1441 attattatta ttatagaagc ccccatgaat aaatcgctca tcattttcgg catcgtcaac
     1501 ataacctcgg acagtttctc cgatggaggc cggtatctgg cgccagacgc agccattgcg
     1561 caggcgcgta agctgatggc cgagggggca gatgtgatcg acctcggtcc ggcatccagc
     1621 aatcccgacg ccgcgcctgt ttcgtccgac acagaaatcg cgcgtatcgc gccggtgctg
     1681 gacgcgctca aggcagatgg cattcccgtc tcgctcgaca gttatcaacc cgcgacgcaa
     1741 gcctatgcct tgtcgcgtgg tgtggcctat ctcaatgata ttcgcggttt tccagacgct
     1801 gcgttctatc cgcaattggc gaaatcatct gccaaactcg tcgttatgca ttcggtgcaa
     1861 gacgggcagg cagatcggcg cgaggcaccc gctggcgaca tcatggatca cattgcggcg
     1921 ttctttgacg cgcgcatcgc ggcgctgacg ggtgccggta tcaaacgcaa ccgccttgtc
     1981 cttgatcccg gcatggggtt ttttctgggg gctgctcccg aaacctcgct ctcggtgctg
     2041 gcgcggttcg atgaattgcg gctgcgcttc gatttgccgg tgcttctgtc tgtttcgcgc
     2101 aaatcctttc tgcgcgcgct cacaggccgt ggtccggggg atgtcggggc cgcgacactc
     2161 gctgcagagc ttgccgccgc cgcaggtgga gctgacttca tccgcacaca cgagccgcgc
     2221 cccttgcgcg acgggctggc ggtattggcg gcgctgaaag aaaccgcaag aattcgttaa
     2281 ctgcacattc gggatatttc tctatattcg cggttcagca ggcatgtccc ctttgagggc
     2341 gacccgacga caggataatc gaccttatgg tgcgcaaata tttcggcaca gacggtattc
     2401 gtggcaaagc caacgaaggc gcgatgacgg cggaaaccgc cttgcgcgtc ggcatggcgg
     2461 ctggccgtgt ctttcgtcgc ggtgaccacc gccatcgtgt cgtgatcggc aaggatacgc
     2521 gcctgtcggg ctatatgctt gaacccgcgc tcacagccgg tttcacctcg atgggcatgg
     2581 acgtattcct ttttggcccg ctgccgacaa cgtataggaa gaataaacgc ccttttcacc
     2641 caagtccaac agctttggac cgcagttgac tctttcgaca cccctgcgat gcaacccaat
     2701 ccggctgacg gggagccagc aacgctgaaa atttaccctc ctctttccca ctagcggctc
     2761 cttttccgac aaccagcacg gcggatccct gccgcggcgc tgtgaacgca gcattttgat
     2821 tggtatcgtt ggccttcagg ctcgtcagtc aaacagaccc aggagcagct cagccggtgg
     2881 cgcccggctt tcgggtaacg ccctggtccc gctggtttcg gctttgtgct tcaggtgatc
     2941 aaggatctgc ttgatcacta tagggtcttc aatgcaggcg atgactttca tggcgccgcc
     3001 gcagccgctg caggtctcga tgtcgatatt gaaaacacgc ttgagccgtt gcgcccatgt
     3061 catcgacgct cgccgttgtg ctggtgttgc cggttcatca gccaccctga ccttgttgcc
     3121 cctgccccgt tttgccggcg tgaccaacgc ccggtgccga ctgttgggtg cgaacacccc
     3181 gtggaagcgg gttaggttga ctctgggctt cggtaccagg gcggccagcc ttgcaatgaa
     3241 atccaatggt tcgaaaatga cgtgcgtggt gccgtcccgg tacggcgtct tgagctggta
     3301 gcgcacgttg ccgcctcgtg ttaacgacag ccgcttctcg gataccgccg ggcggctgat
     3361 gtaccggcac agccgttcga gcttcttgcg ttcatcggcc ctggccgcca cgccggcgtg
     3421 caggctggac ccggctacct tgccaatccc gtcaccgaac ggatcaccac tggtcggcag
     3481 agtttgcaaa gtgaacacct ttcgccccgc ctgtgaaccg acagcgatac ggtaagtgat
     3541 cgagtgcccc agcaggggtg tcatcgggtc gtcatccacc gcatccgagg ccagatagct
     3601 gttttcgaca tcccgttcca gcaggccttg ccgttccaga tagcgaccca cccggtgggc
     3661 gatggtgtgc gtcagctggg tgagctctgg gctggtcggc gccttgaccc agcggaaacg
     3721 cgctgagccg tgggattgct cgacatacac accgtcgaga aacagcatgt ggaagtgaac
     3781 attcagattg agcgccgatc caaaacgctg gatcagggtg accgcgcccg tcttggccac
     3841 ttggtgggta tggcccgctt tcttgaccag gtgcgtggca atgacgcggt aaacgatgcc
     3901 cagcacccac cccatgatct cgggccggct ggcaaacagg aaacgcagct gaaacgggaa
     3961 gctcaacacc cactgacgca tgggttgttc aggcagtact tcatcaacca gcaaggcggc
     4021 actttcggcc atccgccgcg ccccacagct cgggcagaaa ccgcgacgct tacagctgaa
     4081 agcgaccagg tgctcggcgt ggcaagactc gcagcgaacc cgtagaaagc catgctccag
     4141 ccgcccgcat tggagaaatt cttcaaattc ccgttgcaca tagcccggca attcctttcc
     4201 ctgctctgcc ataagcgcag cgaatgccgg gtaatactcg tcaacgatct gatagagaag
     4261 ggtttgctcg ggtcggtggc tctggtaacg accagtatcc cgatcccggc tggccgtcct
     4321 ggccgccaca tgaggcatgt tccgcgtcct tgcaatactg tgtttacata cagtctatcg
     4381 cttagcggaa agttctttta ccctcagccg aaatgcctgc cgttgctaga cattgccagc
     4441 cagtgcccgt cactcccact cttacattgt tcgcagtatc taggtacatg ttattgcgtg
     4501 caccttcatg atgtataatg ttattaatga cagcagaaag atcgacagaa ttaatattct
     4561 tcgtacctgg gacagttatc gggtgtcggc cagtgatgag accaaaggat gcagtataag
     4621 atgaaccact actattatca tggccaccat taccaccacc gttaaaacta cctcccgaac
     4681 ttccagagcc gctatgacca tcattgtagg ggtcatttgg tcccatgtga ccaaaaccac
     4741 cgacagtacc gctatttaac tctgtactca taaaattatc ctctttgact tttaaaaaca
     4801 gcaaaaactg tatttataac aatgtgttta tatacagtat tcgaaatact acgcccgtca
     4861 aaaccacacc gtcaacaggt tttttgtgta tttttcacgc ctcacgggca gcctcagacc
     4921 ttttgtgtta aaacagtaat atgttctgct gttacaatac ggtacacaca ggagacactg
     4981 tcacttttat ggcatggcac acagttcaag agacactgaa attaacagga aagtcgcgct
     5041 cgcaattata tcgtgacatg gctaaaggcc ttgtcagcta tcgcacagga aaaaatgatc
     5101 gccgggagtt cgagaccagt gaactgattc gggcctatgg cgagctgcgc acttctgaga
     5161 caccaaaaag gcacaaggag ggacactcca aatctgtcgt tagccaggcg gaaaatgctc
     5221 agctagacgt cattcagcag aaactggacg ctctgcagca gactgttacc ctaatgcttg
     5281 aagacaagat ggcgcgggaa gcagaaaacc gcctgcgaaa tgaggagagg gaacaactac
     5341 aggccgaaat tagccgtctg gctgacgcgc tggcacagga aaaaaagcgg ggattctggt
     5401 cgcggctgtt tcgctccggt tcagagtaac agtgcaggtg actctgattt accataacat
     5461 ctgttatcag caccgcctga gaggccctca gggcgtttct gacgggaaac cggtaaaaca
     5521 ggcaacaaac cacccgaaaa cccgccagaa acgcgcaggc tctgttttta cggcatgcat
     5581 gagtatgcgc acaagtgcat agcgcggaaa ttcacaccat tcagccgggg taagtcctgt
     5641 ttccggcgtt cttcccatcc gaaaacggtg aacggggacg caatttattg cgtggccgtg
     5701 aagcgttgcc ggcgcagccg gaaaacccgc tgtcagcacg ttcaggaagg gggtgatgta
     5761 caaatggcac agaggatgaa agtgacgtat gagctgaagg aatgatgtaa aagtgatgta
     5821 tgaactgaag gagcgatgta attctgtagt caggaagaat aacccgttca tgcaggcatg
     5881 aacgatacag tgaggaacgc tttttttgtc acgatctccc cttaacctgc gtaaatcacc
     5941 cgccatggtt gattattttt caacctcaaa atggtcatac acctaacgca gccgcgattt
     6001 tcatcgcaac tgcatgaatt aaaag
//
LOCUS       DAZPDU010000228         5952 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZPDU010000228 DAZPDU010000000
VERSION     DAZPDU010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5952
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.231"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..1508
                     /gene="tssI"
                     /locus_tag="ACHURS_004071"
     CDS             <1..1508
                     /gene="tssI"
                     /locus_tag="ACHURS_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFR1239678.1"
                     /translation="CISQFRYSAQIRPSSVVTKDYTFKRPGWPGRFDQEGQYQDYQRT
                     QYEVYDYPGRFKGAHGQNFARWQMDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQAN
                     LNREWQVVASDLHGEQPQAVPGRRGSGTTLENHFAVIPADRTWRPQPLMKPLVDGPQS
                     AVVTGPAGEEIFCDEHGRVRVKFNWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRV
                     GQEVIVDFLNGDPDQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFD
                     DATGKEQVYIHAQKNMNTEVLNNRTTDVINNHAETIGNNQMIAVTNNQIETVGVNQIE
                     TVGVNQIETVGSNQIIKVGSVQVETIGLVRALTVGVAYQTTVGGIMNTSVALMQSSQI
                     GLHKSLRVGLGYDVKVGNNVTFTVGKTKKDDTGQTAIYSAGEHLELCCGKARLVLTKD
                     GQIFLNGTKIHLQGKEQVNGDSLLINWNCAASKSPPKTPDEKQDTPDMREY"
     gene            1576..5571
                     /locus_tag="ACHURS_004072"
                     /pseudo
     CDS             1576..5571
                     /locus_tag="ACHURS_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT         1425 a         1508 c         1925 g         1094 t
ORIGIN      
        1 tctgcatcag ccagtttcgc tacagcgcac aaatccgtcc ttcttccgta gtgaccaaag
       61 actacacctt taaacgtccc ggctggccag ggcgttttga tcaggaaggc cagtaccagg
      121 attaccagcg cacgcagtat gaagtgtatg actaccccgg acgtttcaag ggtgcccacg
      181 ggcagaactt tgcccgctgg cagatggacg gctggcgaaa caacgcagaa gtggcgcgcg
      241 gaacaagccg ttcgccggag atatggccgg gacggcgaat tgtgctgacg gggcatccgc
      301 aggcgaacct gaaccgggaa tggcaggtgg tggcgagcga tcttcacggc gaacagccgc
      361 aggcggtacc gggacgcagg ggttcaggca ccacgctgga gaaccatttt gcggtaatcc
      421 cggcagacag aacatggcgg ccacagccgt tgatgaaacc gctggtggat ggcccgcaga
      481 gcgccgtcgt gacgggaccg gcaggcgagg aaatcttctg tgatgaacat ggccgcgtgc
      541 gggtgaaatt taactgggac cgctataacc cgtcaaacca ggacagttca tgctggatcc
      601 gtgtggcaca ggcgtgggca ggcaccggct ttggcaacct ggcgataccg cgtgtgggtc
      661 aggaggtgat tgtggacttc ctcaacggcg acccggacca gccgattatt atggggcgta
      721 cctaccacca ggaaaaccgc acacccggca gcctgccggg gacgaagacg cagatgacca
      781 ttcgttcgaa aacctataag ggcagcgggt ttaatgaact gaagtttgac gatgcgacag
      841 ggaaagaaca ggtctacatc cacgcgcaga agaacatgaa caccgaggtg ctgaataacc
      901 gcaccacgga tgtgataaac aaccatgctg aaaccattgg caacaatcag atgattgcgg
      961 ttaccaacaa tcagatagag acggtgggcg ttaaccagat agagacggtg ggcgttaacc
     1021 agatagagac ggtgggcagt aaccagataa ttaaagtggg ttctgttcag gtggaaacga
     1081 ttggacttgt tcgtgcgctg acggtgggcg tggcgtatca gacgacggta ggtggcatta
     1141 tgaacacctc ggtggcactg atgcagtcct cgcagatcgg tttgcataaa tcgttgaggg
     1201 tcgggctggg ttatgacgtc aaagtcggaa ataacgttac cttcaccgtt ggtaaaacga
     1261 aaaaggatga taccgggcag accgcgattt actccgccgg tgagcatctg gagctctgct
     1321 gtggtaaggc aaggctggtg ctgacgaagg acggacaaat ttttctcaac ggcacaaaaa
     1381 ttcatttgca gggtaaggag caggttaatg gtgactcact gttgattaac tggaactgtg
     1441 cagcctcgaa atccccaccg aagacccctg atgaaaagca ggatacgccg gatatgagag
     1501 agtactgatc ccgtacctgt tcggtattca gtaacaaacc agcattaaaa ataaacagaa
     1561 gaaggaattt gaatcatgag cggaaaacca gcggcgcgtc agggagatat gactcagtat
     1621 ggcggtccca ttgtccaggg ttcggcaggt gtaagaattg gcgcgcccac cggcgtggcg
     1681 tgctcggtgt gtccgggcgg gatgacttcg ggcaacccgg taaatccgct gctgggggcg
     1741 aaggtgctgc ccggcgagac ggaccttgcg ctgcccggcc cgctgccgtt cattctctcc
     1801 cgcacctaca gcagctaccg gacgaagacg cctgcaccgg tgggcgtttt cggccccggc
     1861 tggaaagcgc cttctgatat ccgcttacag ctacgtgatg acggactgat actcaacgac
     1921 aacggcgggc ggagcattca ctttgagccg ctgctgccgg gggaggcggt gtacagccgc
     1981 agtgagtcaa tgtggctggt gcgcggtggt aaggcagcac agccggacgg ccatacgctg
     2041 gcgcggctgt ggggggcgct gcgccggata tccggttaag cccgcatctt tacctggcga
     2101 ccaacagcgc acaggggccg tggtggatac tggggggggc tgagcgggtg ccgggtgctg
     2161 aggacgtact gccagcgccg ctgccgccgt accgggtgct taccgggatg gcggacgctg
     2221 acgtaccggc gtgaggccgc cggtgacctg gccggggaaa tcaccgacgt gacggacggt
     2281 gccgggcggg agttccgtct ggtgctgacc acgcaggcgc agcgtgcgga agaggcccgc
     2341 acctcttcgc tatcttcttc tgacagttcc cgccctctct cagcctcagc gttccccgac
     2401 acactgcctg gtaccgaata cggccccgac aggggtatcc gcctttcggc ggtgtggttg
     2461 atgcacgacc cggcataccc ggagagcctg cccgctgcgc cactggtgcg gtacacgtat
     2521 acggaagccg gtgaactgct ggcggtatat gaccgcagca atacgcaggt gcgcgctttc
     2581 acgtatgacg cgcagcaccc gggccggatg gtggcgcacc gttacgcggg aaggccggag
     2641 atgcgctacc gctacgacga tacggggcgg gtggtggagc aactgaaccc ggcagggtta
     2701 agctaccgct atctttatga gcaggaccgc atcaccgtca ccgacagcct gaaccggcgt
     2761 gaggtgctgc atacagaagg cggggccggg ctgaaacggg tggtgaaaaa agaactggcg
     2821 gacggcagcg tcacgcgcag cgggtatgac gcggcaggaa ggctcacggc gcagacggac
     2881 gcggcgggac ggcggacaga gtacggtctg aatgtggtgt ccggcgatat cacggacatc
     2941 accacaccgg acgggcggga gacgaaattt tactataacg acgggaacca gctgacggcg
     3001 gtggtgtacc cggacgggct ggagagccgc cgggaatatg atgaaccggg caggctggta
     3061 tcggagacat cgcgcagcgg ggagacagta cgctaccgct acgatgacgc gcacagtgag
     3121 ttaccggcga cgacaacgga tgcgacgggc agcacccggc agatgacgtg gagccgctac
     3181 gggcagttgc tggcgttcac tgactgctcg ggctaccaga cccgctatga atacgaccgc
     3241 ttcggccaga tgacggcggt tcaccgcgag gaaggtatca gcctttaccg ccactatgac
     3301 aaccgtggcc ggttaacctc ggtgaaagac gcacagggcc gtgaaacgca gtatgagtac
     3361 aacgccgcag gcgacctgac tgccgttatc accccggacg gcaaccggag cgagacacag
     3421 tacgatgcgt ggggaaaggc ggtcagcacc acgcagggcg ggctgacgcg cagtatggag
     3481 tacgatgctg ccggacgtgt catcagtctg actaacgaga acggcagtca cagcgacttc
     3541 agctacgatg cgctggaccg gctggtacag cagggcggct ttgacgggcg gacgcaacgt
     3601 tatcattatg acctgaccgg aaaactcacg cagagtgagg atgaggggct tgtcaccctc
     3661 tggcactacg atgcgtcgga ccgcatcacg caccggacgg tgaacggcga cccggcagag
     3721 cagtggcagt atgacggcca cggctggctg agagaaatca gccacctgag cgaaggccac
     3781 cgtgtcgctg tccactacgg ctacgacgat aagggccgcc tgacgggcga acggcagacg
     3841 gtggagaacc cggagacggg ggaactgctg tggcagcatg agacgaaaca cgcctacaac
     3901 gaacaggggc tggcaaaccg cgtcacgccg gacagcctgc cgccggtgga gtggctgacc
     3961 tacggcagcg gttacctggc gggcatgaag ctgggcggga cgccgctggt cgagtatacg
     4021 cgggacaggc tgcaccgtga gacggtgcgc accctcgcac cggacctgga catccgcatc
     4081 ccgtatgcca ccgacccggc gggaaaccgc ctgccggacc cggagctaca cccggacagc
     4141 acgctcacgg cgtggccgga taaccgcatc gcagaggatg cgcactatgt ttaccactat
     4201 gatgaatacg gcagactgac ggagaagacg gaccgcatcc cgacgggtgt gatacggacg
     4261 gacgacgagc ggacccacca ctacgacagc cagcaccgcc tggtgttcca cacgcggata
     4321 cagcatggcg agccactggt cgagagccgc tacctctacg acccgctggg acggcgaatg
     4381 gcgaaacggg tatggcggcg ggagcgtgac ctgacggggt ggatgtcgct gtcgcgtaaa
     4441 ccggaggaga catggtacgg ctgggacggc gacaggctga cgacggtaca gaccgacacc
     4501 acacgtatcc agacggtata cgagccggga agcttcacgc cgctcatccg ggtcgagaca
     4561 gagaacggcg agcgggagaa agcgcagcgc cgcagcctgg cggagaagct gcagcaggaa
     4621 gggagcgagg acgggcacgg cgtggtattt ccggctgaac tggtgcggct gctggacaga
     4681 ctggaggaag aaatccgggc agaccgcgtg agcagtgaaa gccgggtgtg gcttgcgcag
     4741 tgcgggctga cggtggagca actggccaga caggtggagc cggaatacac accagtgcga
     4801 aacgttcatc tttaccactg cgaccatcgg ggccagccgc tggcgctcat cagcgaagac
     4861 ggcgatacgg tgtggagcgg ggcgtatgat gagtggggca accagctaaa tgaggagaac
     4921 ccgcatcatg tgtatcagcc ataccgtctg ccggggcagc agtatgatga ggagtcgggg
     4981 ctgtactaca atcgtcaccg gtactatgat ccgttgcaga gacggtacat cacccaggac
     5041 cccattgggc tgaaaggtgg gtggaatctg tatcagtatc cgctgaatcc ggtgagcagg
     5101 atagatccgt tggggttatt ctcatgtgag agttgtagtc attttataga tgatataaat
     5161 aataaactgg ataaccttcc gtttaacccg acaaaaatgt ttacagctag tttaaatact
     5221 gctaattcag caaggctgta tgcaagtggc atgacaaaat tcggtataag tgttgctgcc
     5281 gatggaacga tcattgctgc cccagtcggt gcagcttcta tggctttagg ggcgtggaat
     5341 ataaaatctg cgacagctgc atataacaga gcaaatttac aattatcaga atcacttcag
     5401 gaaaatggta gagataggtg ttggagaaat ttattgggta ttttacctta tggtgagcag
     5461 tttgatgacc ctggtgaacc atcaatatta gacgtttatg gtgaaaagat tgagaatatc
     5521 aaagattctc caatgaagtt ttttgaagag cttgggacgc tggggttgta atgtgtattt
     5581 ttaatgatat ttttatgttt gtgtttattg ttagtagtat acttgtaacc gtgcattttg
     5641 tgtattcttt attttttaaa agtggaaagg taaaagataa ggtaaacaaa tggattaaag
     5701 taatgtgtga tttattttgg gggataggat agtggtgtga tatggataaa tataaattac
     5761 agtggctggc atatttcgac ctgacgccgg agtagataaa aaaacagaca ggattggagt
     5821 atagcaagcg tctgacaatc tctcgggaca gtagtatgat gaagaggcgg ggttgtacta
     5881 taaccgtaac cggtactacg atccgctaaa gggcaagtat atgactcagg gaaagatcat
     5941 cagaagttca ct
//
LOCUS       DAZPDU010000229         5931 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZPDU010000229 DAZPDU010000000
VERSION     DAZPDU010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5931
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.232"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            292..368
                     /locus_tag="ACHURS_004073"
     tRNA            292..368
                     /locus_tag="ACHURS_004073"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:326..328,aa:Arg,seq:tct)
     gene            complement(413..1174)
                     /gene="envY"
                     /locus_tag="ACHURS_004074"
     CDS             complement(413..1174)
                     /gene="envY"
                     /locus_tag="ACHURS_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFR1239679.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENISYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(1357..2247)
                     /locus_tag="ACHURS_004075"
     CDS             complement(1357..2247)
                     /locus_tag="ACHURS_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFR1239680.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYIRAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            complement(2248..5220)
                     /gene="nfrA"
                     /locus_tag="ACHURS_004076"
     CDS             complement(2248..5220)
                     /gene="nfrA"
                     /locus_tag="ACHURS_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFR1239681.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLNEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPSAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGSNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKRLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            complement(5207..>5931)
                     /gene="nfrB"
                     /locus_tag="ACHURS_004077"
     CDS             complement(5207..>5931)
                     /gene="nfrB"
                     /locus_tag="ACHURS_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase family protein"
                     /protein_id="HFR1239682.1"
                     /translation="DTALRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESID
                     AWQIPSSLIAEMPASVALHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRY
                     FIVLRGQIVTGLRHWYARRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAE
                     ILTTLGHINRSAINVLLLRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQS
                     LLLKAGLNTEQVAQLESENEGE"
BASE COUNT         1346 a         1561 c         1549 g         1475 t
ORIGIN      
        1 ttctgatgat aggatgagta tccataatga tcaccgacgt tggtttcata gttaccagtc
       61 tcataggagc ggacaatttt ccgttagggc ggtaaattgt actttgatac atgaaaatac
      121 gggttttctt gattcaaacg cgcagcggtg tgcatttgtt tgccgctata gcgtaataaa
      181 tcagaaaatc agacgcggtc gttcacttgt tcagcaacca gatcaaaagc cattgactca
      241 gcaagggttg accgtataat tcacgcgatt acaccgcatt gcggtatcaa cgcgccctta
      301 gctcagttgg atagagcaac gaccttctaa gtcgtgggcc gcaggttcga atcctgcagg
      361 gcgcgccatt tcttcttcat ttaaccccgt cttatccgtt ccgctttgct cttcaccaca
      421 tcactttttg tcgctgtttg gcgagataat tcaacggtgt caggccgtaa aacgccttaa
      481 aaacagagat aaagtacgac gtgctgctat agccacataa ttgcgccacc tgagtgatat
      541 ttttgttatc catcaataac atctgtacgg cgtaacgcat acgacactct gtgacaatct
      601 ggctatagct gatattttcg ttttttaatt tctttttgag caggctgggg cttaaacata
      661 gcgaactggc gacaattcgc agattccagt aatgctgaat atcgctttga ataatgcggc
      721 agacgctgtc gcggacgctg ctgcgtaaga tatacatcag tagggcaata aaccgcgatt
      781 gctcaagaaa gttagacaat acggtaaaaa gcaatgcgcg cgtcaactcc gcttcgcagg
      841 gtttgctggg ttgctgcttg ctgtggttgg cggctagccg gaacacttcc ggtgtacggc
      901 tatgacaagc gataaccggg gttgccagcc gcgaccaggg ctttacacag gttaagttct
      961 tatttaaaaa caacagataa tcgttgatga tgttacggtt gatgtgggtg attaatacgt
     1021 gatttaacgt tgagagttca atgacgttat tgttgcacgc gaaggcggcc aggtagtttt
     1081 tcggaagggt aaacgtagca tggttattga cgcttacctc tacctctttt tcggtcagga
     1141 tcaccacgca aggttcactg ctgctcaatt gcatttcgca ctcctcagat atcagaaact
     1201 ccgctcaaag gatctatgct tcctgcatga gtgatcggcc cgttcgccga taacgatctt
     1261 ctttctttag cacgcttttt agcaattaat cttgatggaa ttctgacgag agcgaaagag
     1321 gtaagccagg tcgtacccga cttacctgga ggagatttaa tactcgagaa tgccgtgcgc
     1381 gacgggcaaa tagcgcagag agaaatagag agtgtcttta ccgcaagagg aacgtttcgc
     1441 taacagcgag gcgatctctg catccggttt cggtttcgct gtaaaggttt tgcctttgcc
     1501 ggcgacaaac agttcataaa caatgccgct tgctggggcg gagctttggc aatcggcgct
     1561 ggcctgtaga taacgttcac gctgttcagc ggtaagttta tccacgccgc tgccatcctg
     1621 tacccagaca ttaactccgg ttgctttaac ttgttccagc agttggcgat agccatctgg
     1681 cgacatgttt ccggcgaaaa aactactgat ataaaccggt tttgctgaaa catcgctaat
     1741 cagccgctgc gcgttgttta accatgttag caaaggctga cgggcagctt cgctgcgcca
     1801 gttcaggtcg tcaatttccg ccctgatgta ccagccatcc ggcgttatgc caggcgcggc
     1861 gctccataat ctggcttgct ggagatcggc agccagcagg cgattaagat agctttccag
     1921 cgctgcggac gactgtttct ggtgcataaa aaattccgga tcggcgttca gcccgacaat
     1981 cagtttcagg ccagcctgtt gcgcagctgc ggcacgctta aacaataacg tgcgctgttc
     2041 tggctgggta aatgcatcgc cgtaacgggt ccattgcaaa accagggtat cgaagccttg
     2101 caaacgtaac tgactcatca gcccctgcca ctgggtatcg gtaacctgac tatctcggtt
     2161 ttgtggttgc cagataatac ctttcatcgc aaaggaaaaa gggctgacca aaagcagtgt
     2221 cagcaatacg aaaatgaact tacgcattta ccagtgcact ccaatggtga gaaacgcgtt
     2281 gttgcgctct ccgttacgtt gattaatcgc cttaaaggta tgttgatact cgacgccgag
     2341 actgactttg tgcggccagg cgtcgtagtg cgtctcgccg gtccagatat tccagcggac
     2401 cccgactccg ccaagctgcg cgccctgagt gcctttatca cgatagccgt tgtcctgaac
     2461 gtgagcgtaa ggctcaatag tctgtccgtt agctaccttc tgatgccagc tgacgcgata
     2521 atctgccgtc cacgcctgaa tatcctggcg gatatattgc gccgcatcga ggtacaggtt
     2581 ttgggcaaac cagcctgaac cgttcgggtg ccattcgtcg ctgtatttgc cgccattaaa
     2641 gaatgaggcg ctggcgcgca gcatggtatc ggatgcgcca ttttgcccgt tcagcggcaa
     2701 ctgctgttcg acggcgagga aaaagatctg atcgcgcagc ggcttccagc gcagaccggt
     2761 gccggacatc ggatttttca ccggcatcat caccccgttt tctccggtat cggcaaagac
     2821 gcggctataa actgagagca ggtcgccttc cagcagcata ttgcgtccga tgcggtactc
     2881 ggcttccagt tgcccgtagc tacgatagct tttccctggc gctgcgccgc caacattatt
     2941 gttagcggta ctcattgccc cggaacgcaa gccgatagaa gaatcgaaac tgaacgtcca
     3001 gcggcgaccg acctcctcat gcaaacggcg gaaattgaag cgttgttgat tttgctctgg
     3061 ggtcagtggg gttatcagcg cctgattatc aatgtcatca atcaccagcc gggcgtagtg
     3121 ctgcgtcgca ggcatgtcat ccagacgctg gttcacgtag gccagttgtc ggatcagtgc
     3181 cggatcgtcc ggaagccttt tatgcgcctg ttcgagcatt tcccgcgact gtgcgatatc
     3241 accgctatcc cacaaggcgt aaccaagcgc tgcctggatg ttgctattat tcggttccag
     3301 ttccagcgcg gcgcgcaaat cactcaccgc ggccgggaca ttatgacgtt ggcgataaat
     3361 tgtcgcccgc gcaacgtaag cgttggcaga aggcgcaata ttgattgagc gcgtgagatc
     3421 gttcagtgcg agttccggct gaccaggaat gtaacgttgt gcatgcagcc accagtagag
     3481 ggcattgctt cccagtccac gttgttctgc ctgttgtagc cagcgatcgc gagccgcacc
     3541 atttcctgcc gcctgggcgg tattggcagc agcaagcaga tcctcattgc tcatgtcgtg
     3601 aagactgatt ttctgccagg ccgccagtgc ggtggcgtag tcctcaacct gatacgcctg
     3661 ataggctacc gcacgatgtt gccaggcgct cggttgtcgt tgttcggcct gaagccatgc
     3721 atacaacgcc acaccgggta gcgtgtcccg ataacacttt gccagacggt tccaggcggc
     3781 agcatcgtag gaaggcgaca tatcgcccag caagcgaact attgccgggc aattatctgc
     3841 aatacccggc aactgacttt gccactgacg ttgctccgcc agcggtaagg gtttcgataa
     3901 aatcgccacc ttcgccggcg ttgccaggta aggatgactt tccagcagag acgccagtcg
     3961 cgccattaaa gtctggctga cacgcgcatc gccctggaaa ggatagcgtt gcagcaataa
     4021 atcggcagct tcgcgtgact gctcgttctg catcagttgc caggtcagtt gatccaggcg
     4081 ggtaagattt gccggttctt gctgatacag caatcgtgcc agacgcagag attcagcctt
     4141 gttacgggtc gccacgctga cagcataacg ctcctcaagc atttcattgg cggggagggt
     4201 ggcgagcagt ttttgcgctg cgtcgtactg accttctttt aacagcaccg gtagcgtcgc
     4261 gccaacaaca tactggcggt tatcggcaaa ctgcaccgta taattcgcca atgcctgaac
     4321 ggggttggcg ctgtatttag ataacagata gagccaactt ttctcttgtg cgtccgtggt
     4381 aaacagcggc ttattttcat tgagataatg ctggaggcgt gctttttcgc cacgataagc
     4441 cagcgcggtc gcgtaagtaa tatatgactg aggatcggtg aagatcccct gtgattgcag
     4501 tgctaggatc cgatcgtcca gctgcccggc aagaagcacg tcaaaccact gacggcgttc
     4561 tgccgcgctt aatgtgttct gctggcgtgc ttcattgtat agcgtatctg cctgggacca
     4621 ttgtttcagg tagattgccc gttgcagcag atcggttcgc agcgtttttc cttccggcga
     4681 tgcagcaaac gtcgcatcgt tcagttgcgc tctggcgaca ggtaactgtg caagccgcag
     4741 ggcattctgc ccgacttcac tgcgacaacg cagggtcggc gcagcatcgc acgctttttg
     4801 ctgggcaagc agttcttcaa cagttgtaac gcttttcact tcaaccggaa tagccgccag
     4861 actgcgctca agtcgggcat ctcctgggtg acgtttcagt tgatcctcaa gcaacagccg
     4921 cgcccggtca tcatgaccaa aatggcgata ggcttccgca aggtataaag tcagcggaat
     4981 attatccggc acctgctggt gtatatattc aaattcgcgg atggcggttg cttcgtcgtt
     5041 atttttctgt gccttcagcg ccttatcgag acggggataa ataacaaaat ggcgataatc
     5101 gctcagcccc agctcttctg cgctggtgcc gatattgtct gcgagtgcgc tggtactcaa
     5161 taaagacgtc agcagtaaac cagaccatcc gatgacgcga ttaaggttat tctccttcat
     5221 tttcggactc cagttgcgca acctgttctg tgtttaaacc tgctttgagt aatagtgatt
     5281 gcatcgaaac ttgtaattcg cgttgaattg tcaggacgcg atccaacgtt tcctggctga
     5341 taacgccttc ggtgaccaaa aacttgccga gcggcagaga actgcgttca tggcgcaata
     5401 acaacacgtt aattgctgaa cgattaatat gaccgagcgt ggtcagtatt tcggcgaaca
     5461 ggaactgatg cggcacatat tgccgccaga tttcaccggc ctgctgttcc gtgagccact
     5521 gatgctgaac cgcattgtac aacattgccc gcggatcgtg accgcgtcgg cgtgcatacc
     5581 agtgacgtaa ccccgtgaca atttgtcccc gcagaacaat gaagtaacgc actttgcgtc
     5641 cgactttacg cgtcagggcc gccagcgaaa ccgggtcaat accatcttca ctgccgacaa
     5701 ttaactcatc attttccaga cgcagcggca gtaccgcata atgcagcgcc acggaggccg
     5761 gcatttcggc aatcagcgag gaagggatct gccaggcatc gatggattcc cacgccacgc
     5821 cgttttgctc tgccagcgcc tgtgccagct gctcggcgct aatcagcccc tgcatcagca
     5881 ttgaaccgcc caagcgtaga ccttcgacgc gattacgcag tgctgtatcg a
//
LOCUS       DAZPDU010000230         5857 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZPDU010000230 DAZPDU010000000
VERSION     DAZPDU010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5857
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.233"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(39..401)
                     /locus_tag="ACHURS_004078"
     CDS             complement(39..401)
                     /locus_tag="ACHURS_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFR1239683.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            938..2851
                     /gene="mtlA"
                     /locus_tag="ACHURS_004079"
     CDS             938..2851
                     /gene="mtlA"
                     /locus_tag="ACHURS_004079"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFR1239684.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            3081..4229
                     /gene="mtlD"
                     /locus_tag="ACHURS_004080"
     CDS             3081..4229
                     /gene="mtlD"
                     /locus_tag="ACHURS_004080"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFR1239685.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISDLDANSEVVSEAVTAYKAMQ"
     gene            4229..4816
                     /gene="mtlR"
                     /locus_tag="ACHURS_004081"
     CDS             4229..4816
                     /gene="mtlR"
                     /locus_tag="ACHURS_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFR1239686.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(4828..5037)
                     /gene="yibT"
                     /locus_tag="ACHURS_004082"
     CDS             complement(4828..5037)
                     /gene="yibT"
                     /locus_tag="ACHURS_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFR1239687.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            5322..5684
                     /locus_tag="ACHURS_004083"
     CDS             5322..5684
                     /locus_tag="ACHURS_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFR1239688.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         1476 a         1450 c         1519 g         1412 t
ORIGIN      
        1 cagcaccacg taagttaaaa caatcaatag atccataatt agatctccgg gaacgtggaa
       61 tagtcagtct ttttctcggc ggcgagttga tgctctagcc gggaaattcg gtcagaaagt
      121 gcggcgattt ccgggtcagt cgctttctcc tgcgacgcaa catgtgactg catgccccac
      181 ccgcgctccg gttgatagag cgtcgcccag atccacagga acggccagat aacatgcagg
      241 gtaaacaggc tcacccagcc cgccgtatga atagcgtcgg catggggatg gttgcgcttt
      301 ttggcaatca gataagggat gtcatgtatc gcgatgattc cataaaaaat caccagaaag
      361 acgaagatca gcactcccaa cgcgaaatag tttagaaaca tatctgcctc ggattcacgt
      421 ttatcagtgt tgtttttggg ctggcagcca gaagggagtc aggctgatat tttgacaata
      481 atccgggttc gcgattctcg ccataacacc aaagaataat ttttagaggt gatgagttgc
      541 ttagttacat aacaattgta tgacgaaggc ataacatgct gtagatcaca tcaggtgaac
      601 gccgtaagaa aatatcttgt gattcagatc acaaagattc aacaaaccat caaaacaaaa
      661 atgtgacact actcacattt aaatgccatt tttagcgaaa atcgccgcct tgttgctttt
      721 ttacacaagc gttttgtgat gaacgtcacg ttaattacct ctctaccccc tatatttatg
      781 tgattgatat cacacaaaag gccgtcgact ggacagttaa ccgattcagt gccagatttc
      841 gcagtatcta caaggtccgg ctacctctgc cgccacatta acaaaaaacc tcgggcttcc
      901 agcctgcgcg acagcaaaca taagaagggg tgtttttatg tcatccgata ttaagatcaa
      961 agtgcaaagc tttggtcgtt tcctcagcaa catggtgatg ccaaatatcg gcgcgtttat
     1021 cgcgtggggt atcatcaccg cgttatttat tccaacaggg tggttaccga acgagacgct
     1081 ggcgaagctg gtcgggccga tgatcactta tctcctgccg ctgctgatcg gttataccgg
     1141 tggtaagctg gtaggcggcg aacgtggcgg cgtagtcggt gccatcacca ccatgggcgt
     1201 tatcgtcggc gcagacatgc cgatgttcct cggttctatg attgcaggtc cgctgggcgg
     1261 ctggtgcatt aagcacttcg accgctgggt agacggtaag atcaaatccg gttttgagat
     1321 gctggtgaat aacttctccg caggcatcat cgggatgatc ctcgctattc tggcattcct
     1381 cggcattggc ccgattgttg aagccctgtc caaaatgctg gctgcgggcg ttaacttcat
     1441 ggttgtccat gacatgctgc cgctggcgtc tatctttgtt gaaccggcga aaatcctgtt
     1501 cctcaacaac gccattaacc acggtatctt ctcgccgctg ggtattcagc agtcccatga
     1561 actgggtaaa tcaatcttct tcctgattga agctaaccca ggtccaggta tgggcgtgct
     1621 gctggcgtac atgttctttg gtcgtggtag cgctaaacag tctgcgggcg gtgcggcaat
     1681 catccacttc ctgggtggta tccacgaaat ctacttcccg tatgtgctga tgaatccgcg
     1741 tctgatcctc gcagtcatcc tcggcggtat gactggcgtg ttcacgctga ctatcctggg
     1801 cggtggtctg gtttctccgg catctccggg ttctatcctt gctgtactgg cgatgacacc
     1861 aaaaggtgct tatttcgcta acatcgcggg tgtgtgtgcg gcgatggctg tctccttcgt
     1921 tgtctctgct attttgctga aaaccagcaa agtgaaagaa gaagatgata ttgaagcagc
     1981 aactcgtcgt atgcaggaca tgaaagctga gtctaaaggc acatctccgc tgtctgcggg
     2041 cgatgtgact aacgacctga gccacgtacg taaaatcatc gttgcctgtg acgccggtat
     2101 gggttccagt gcgatgggcg caggcgtgct gcgtaagaaa attcaggatg caggtctgtc
     2161 acagatttcg gtcactaaca gcgcgatcaa caacctgccg ccagatgtgg acctcgtcat
     2221 cactcaccgt gacctgaccg aacgcgctat gcgccaggtt ccgcaggcgc agcatatttc
     2281 gctgaccaac ttcctcgaca gcggcctgta caccagccta accgaacgtc tggttgctgc
     2341 ccagcgccat actgaaaacg aagtgaaagt gaaagacagt ctgaaagaca gctttgacga
     2401 ttccagtgct aacctgttca aactgggcgc ggagaacatc ttcctcggtc gcaaagcagc
     2461 aaccaaagaa gaagcgattc gttttgctgg cgagcagctg gtgaaaggcg gttacgttga
     2521 gccggaatac gttcaggcga tgctggatcg tgaaaaactg accccgactt atctgggtga
     2581 gtctatcgcg gtgccacacg gtacggttga agcgaaagat cgcgtactga aaacgggcgt
     2641 ggtgttctgc cagtacccgg aaggcgtgcg cttcggtgaa gaagaagatg acattgcccg
     2701 tctggtgatt ggtattgcag cccgtaacaa cgagcacatt caggttatca ccagcctgac
     2761 caatgcactg gatgatgagt ccgtcatcga gcgtctggca cacaccacca gcgtggatga
     2821 agtgctggaa ctgttggcag gtcgtaagta atccaatccc accctctcca catggagaag
     2881 gtggggttaa ttgcctgatg cgctacgctt atcaggccta caggatgcat cacgatttgt
     2941 tgaatttgca cgttcttgta ggccggataa ggcgcttacg ccgcatccgg cgctgcccct
     3001 ctcctcacgg agagggtttg ggtgagggaa aagcctcacc ccagccctct cgggtaaaaa
     3061 cattgatgaa ggttaatact atgaaagcat tacattttgg cgcaggtaat atcggtcgtg
     3121 gctttatcgg taaactgctg gcagacgcgg gtatccaact gacgtttgcc gatgtcaatc
     3181 aggtggtact tgatgccctg aatgcccgtc atagctatca ggttcatgtg gttggtgaaa
     3241 ccgagcaggt agataccgtt tccggcgtca atgctgtcag cagcattggt gatgatgtcg
     3301 ttgatctgat tgctcaggtc gatttagtca ctaccgccgt tggcccggtt gtgctggaac
     3361 gtattgctcc ggctatcgcc aaagggctgg tgaaacgcaa agaacaaggt aatgaatccc
     3421 cgctgaacat catcgcctgt gaaaacatgg tacgcggtac cacgcagctg aaaggccatg
     3481 tgatgaacgc cctgccggaa gacgccaaag cgtgggtaga agaacacgtt ggctttgtcg
     3541 attccgccgt tgaccgcatc gtaccgcctt cggcttcggc aactaacgat ccgctggaag
     3601 tgacggtaga aactttcagc gaatggattg tcgataaaac gcagttcaaa ggcgcactgc
     3661 cgaacatccc aggcatggag ttaaccgaca acctgatggc atttgtcgaa cgtaaactct
     3721 tcaccctgaa cacgggtcat gctataaccg cgtacctcgg aaaactggcc ggtcatcaga
     3781 ccattcgtga cgctattctc gacgagaaaa tccgcgcggt ggtaaaaggt gcgatggaag
     3841 aaagtggtgc ggtactgatc aagcgctacg gctttgacgc agacaaacat gcggcgtaca
     3901 tccagaaaat cctcggtcgt tttgagaacc cgtatctgaa agatgatgta gagcgcgtag
     3961 gccgtcagcc gctgcgtaaa ctgagtgctg gcgaccgtct gatcaagcca ttgctcggta
     4021 cgctggaata cagccttccg cacaagaatc tgattcaggg gattgctggt gcaatgcact
     4081 tccgcagtga agatgatccg caggctcagg aactggcagc actgatcgct gacaaaggtc
     4141 cgcaggcggc gctggcacag atttccgatc ttgatgccaa cagcgaggtt gtatccgagg
     4201 cggtaaccgc ttataaagca atgcaataat ggtggaccag gcgcaggaca ccctgcgccc
     4261 gaataacaga ttgtcagata tgcaggcaac aatggaacaa acccaggcct ttgaaaaccg
     4321 tgtgcttgag cgtctgaatg ctggcaaaac cgtgcgaagc tttctgatca ccgccgtcga
     4381 gcttttgacc gaggcggtaa atcttctggt gcttcaggta ttccgcaaag acgattacgc
     4441 ggtgaagtat gctgtagaac cgttactcga cggcgacggt ccgctgggcg atctttctgt
     4501 gcgtttaaaa ctcatttacg ggttgggcgt cattaaccgc caggaatacg aagatgcgga
     4561 gctgctgatg gcattgcgtg aagaactaaa tcacgacggc aacgagtacg cctttaccga
     4621 cgacgagatc cttggaccct ttggtgaact gcactgcgtg gcggcgttac caccgccgcc
     4681 acagtttgaa ccagcagact ccagtttgta tgcaatgcaa attcagcgct atcaacaggc
     4741 tgtgcgatca acaatggtcc tttcactgac tgagctgatt tccagaatca gcttaaaaaa
     4801 agcctttcaa aagtaagcaa cgtctgctta ctgcccctct acctgcttcg gccgataaag
     4861 ccgacgataa tactccagac gttgtagata taacggcacg ctttcatcgg gtattccgga
     4921 cggaatcgcg ttacggggag gaagtttttt cagatactcc cggaacgcct ggcttgatgc
     4981 catgaaatct acggctttat cgataagaag cggaacgttt tcacctaatt tgcccatgat
     5041 gatatctccg tattaccccg cgccgggaat gcgcggccgc caattttagt ttaggttccc
     5101 ggtaaagatg acattaagga aaacgtgctg aatcctcaat aatcatccgc tgctgtttat
     5161 tatctttttt ctatagattt cttaattaat caataaattg cgtagttttt aagcatgtta
     5221 atgattacgt tacagaacta ttgcaatgtc atcagttgtg aacaaagcac ctggtcgcgc
     5281 atactagggg ctataaattt atctttatca gaagccatca catgaaagaa gtcgaaaaaa
     5341 acgaaatcaa acgtctcagc gatcgtctgg acgccatccg ccaccagcag gccgatctgt
     5401 cgctggttga agccgcagac aaatatgccg agctggaaaa agagaaagcc acgctggaag
     5461 cagaaattgc tcgcctgcgt gaagttcata gccaaaaact gagtaaagaa gcacagaagc
     5521 tgatgaagat gccgtttcag cgcgcaatta ccaaaaaaga gcaggctgat atgggcaagc
     5581 tgaagaaaag tgttcgcgga ctggtggttg tgcacccaat gaccgcgctg ggccgtgaaa
     5641 tgggcctgca ggagatgact ggattctcta agactgcgtt ttaagcgcct cagacgactg
     5701 gttcgatcat atcaacccgc atctggcttg ccttagctgg tcaatgcggg gactctcctg
     5761 tctactcgcc taattaccaa acaattcact ccctttacgt cgatttaccg ctggttgata
     5821 ttctgaaaat gcatttttac cgaaatagcc acctata
//
LOCUS       DAZPDU010000231         5678 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZPDU010000231 DAZPDU010000000
VERSION     DAZPDU010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5678
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.234"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..661)
                     /locus_tag="ACHURS_004084"
     CDS             complement(<1..661)
                     /locus_tag="ACHURS_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1239689.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKEQEIAVAGNI
                     IL"
     gene            761..1351
                     /locus_tag="ACHURS_004085"
     CDS             761..1351
                     /locus_tag="ACHURS_004085"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HFR1239690.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTPEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPVWWYSFPAMLKGYI
                     DRVWNNGLAYGDGQKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGEELHASYYQSLLSQVRDMVDAL"
     gene            complement(1433..2173)
                     /gene="pflA"
                     /locus_tag="ACHURS_004086"
     CDS             complement(1433..2173)
                     /gene="pflA"
                     /locus_tag="ACHURS_004086"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFR1239691.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(2365..4647)
                     /gene="pflB"
                     /locus_tag="ACHURS_004087"
     CDS             complement(2365..4647)
                     /gene="pflB"
                     /locus_tag="ACHURS_004087"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFR1239692.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(4702..5559)
                     /gene="focA"
                     /locus_tag="ACHURS_004088"
     CDS             complement(4702..5559)
                     /gene="focA"
                     /locus_tag="ACHURS_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFR1239693.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT         1462 a         1335 c         1413 g         1468 t
ORIGIN      
        1 ccagaatgat attccctgcg acagcaattt cctgctcctt tgcaaattgc cagtggaaag
       61 gtttgccgct gggataacga aatacaacac attgatgatt aagcagatca tgaggatggc
      121 ggggtttgcc atatcttgca aagtactctg gcgttgctgc tacacataat ttcactggtg
      181 ggccgatcgc cacgctgatc atatcttttt cgacgatgcc actaagtcga acgccagcgt
      241 caaagccctg ttggacgata tcgacaagag agtcgtcagt tgtgagctcg actttaatat
      301 caggatattc acgtgtgaaa ccaaccagta aaggcattaa aaatatacgt gcagctaccc
      361 gggcggcatt gattttgagc gttcctgttg gtgtcagacg aaaatcattc atctcatcta
      421 acatgatctg aatttcgtcg aaagctgggc gcaatcgttc atagagatta gaacccgctt
      481 ccgttaacga tacgcttcgg gttgtccgat tgaataagcg aattttaaga cgttgttcca
      541 gtgttttaat actatggcta atggcggacg aggataagcc taactcatcg cccgctgcac
      601 gaaagctttg attacgggcc acagcaaaga aagtggcaaa gtcagacata ttcatccgca
      661 ttagtgaaaa tccttcatta actcatcctg aatcatcacg tttacatgat tatgccgcct
      721 gcgtaaagtg cgctccagaa cttaacgtgg aggtaaaatt atgcagtctg aacgtattta
      781 tttggtatgg gcccatcctc gtcatgattc attgaccgca catattgctg atgcgatcca
      841 tcagcgggca atggagcgga aaatacaggt gacggaactt gatttatatc ggcgtaattt
      901 caacccggtg atgacgccgg aagatgaacc agactggaag aatatggata aacgttattc
      961 tccagaggtt catcagcttt attcagagct gcttgaacat gacacgttag tggtggtttt
     1021 tcctgtctgg tggtacagct tcccggcaat gctaaaagga tatattgaca gagtatggaa
     1081 taatgggctg gcttatggag atgggcagaa attaccattc aataaagttc gttgggtggc
     1141 gctggttgga ggagacaaag aatcatttgt ccagatgggc tgggaaaaaa atataagcga
     1201 ttatttaaaa aatatgtgca gttatcttgg tattgaagat gccgatgtca ctttcttgtg
     1261 taatacagtg gtattcgatg gggaagaact tcacgcgagc tattatcagt cgttattatc
     1321 tcaggtacgg gatatggtag atgcactata agatgtgtta aaaacgctgt agcagaatga
     1381 agtgcggaat aaaaaagcgg caactcaata aagttgccgc tttacgggga aattagaaca
     1441 ttaccttatg accgtactgc tcaagaatgc ctttcacgcg ttccatggtc tctttcttcg
     1501 gtggtttaac accgtcgagt ttgtactctt cacccattgc cacccatttg tgtttgccca
     1561 gctcgtggta ggggagaagc tcgattttct caacgttgcc catatcacgg gtaaattcac
     1621 cgaggcgatg cgctgaatcg tcatcgtcag accagcctgg gacaacaacg tagcggatcc
     1681 acaccttcac atttttgttc gccagatatt tggcgaactc cagcgtgcgg tgattggaaa
     1741 ctccaaccag attttggtgg atctcgtcgt tcatctgttt gagatcgagc attaccaggt
     1801 cggttacttc cagcagttca tcaatcaccg gatcgtaacg acgaacaaaa ccgttggtgt
     1861 ccagacaggt atgaatgcct tcttttttgc aggcgcggaa ccagtcacga acaaactcag
     1921 cttgtaggat tgcctcaccg ccggatgcgg taacgccgcc gccggaagcg ttcataaagt
     1981 ggcgataggt caccacctct ttcattaaat cttcaacggt aacttcttta ccgccatgcg
     2041 tatcccaggt gtcgcggtta tgacaataca ggcagcgcat caggcagccc tggaaaaagg
     2101 tgataaagcg aatacccggg ccgtctacgg ttccacagga ttcaaaggag tgaatgcgac
     2161 caataactga cattgcggtg tttctccaga tgtggcccat ctgaggccgt gttggtgcgc
     2221 agctcgaagg ctacgtcgag tctgttttgg cagtcacctt agagtataga tagctgacaa
     2281 aaaaggctct cgtgctaaaa aaggccccac tttcgtggag cctttattgt acgcttttta
     2341 ctgtacgatt tcagtcaaat ctaattacat agattgagtg aaggtacgag taataacgtc
     2401 ctgctgctgt tctttagtca gcgagttgaa acgtactgcg tagccagata cacggatggt
     2461 cagctgcgga tatttttccg ggttttccat cgcgtcgagc agcatttcac ggttcatcac
     2521 gttaacgttc aggtgctgac caccttcgat ggatgcttcg tggtggaagt aaccatccat
     2581 cagaccagcc aggttggtct tacgaacttc gtcgtcttta cccagtgcgt tcggaacgat
     2641 agagaaggtg taggagatac catctttagc gtaagcaaac ggcagtttag caacggaagt
     2701 cagagaggct actgcacctt tctggtcacg accgtgcatc gggttagcac ccggtccgaa
     2761 cggcgcgcca gcacgacgac cgtctggggt gttacccgtt ttcttaccat acacaacgtt
     2821 agaagtgatg gtcagaacag actgagtcgg gatagcgtca cggtaggtgt gcagtttctg
     2881 aattttcttc atgaaacgtt ctaccaggtc aacagccagg tcatctacac gcggatcgtt
     2941 gttaccaaac tgcgggtatt cgccttcgat ttcgaagtcg atagccagac cgtcttcgtc
     3001 acgaatcggt ttaactttcg catatttgat tgcagacagg gagtcagcag caacggacag
     3061 accagcgata ccacacgcca tggtgcggat aacgtcacgg tcgtgcagcg ccatcagaga
     3121 ggcttcgtag ctgtacttgt cgtgcatgta gtggatgatg tttagtgcag tgatgtactg
     3181 tttagccagc cagtccatga agtgatccat gcgctccatc acttcatcat agttcaggac
     3241 atcgcctttg atcggttcag acttcggacc aacctgcatt ttcagttttt cgtcaacgcc
     3301 gccgttgatt gcgtacagca tggttttcgc caggtttgca cgcgcaccga agaactgcat
     3361 ttgtttacca acgatcatcg ggcttacgca gcaagcaata gcgtagtcat cgttgttgaa
     3421 gtccggacgc atcaggtcat cgttctcata ctgcagagaa gaggtgtcga tggacacttt
     3481 agcggcgaat ttcttgaagt tcagcggcag tttttcagac cacagaatgg tcatgttcgg
     3541 ttccggagac ggacccatgg tgtacagggt gttcaggaaa cggaagctgt ttttggtaac
     3601 cagggtacga ccgtcgaggc ccataccacc gatagattcg gttgcccaga tcgggtcgcc
     3661 agagaacagt tcatcgtatt ccggagtacg caggaagcga accatacgca gtttcatgac
     3721 caggtggtca accatttcct gcgcttcttg ttcggtgatc ttgccagctt tcaggtcacg
     3781 ttcgatgtac acatccagga aggtggaggt acgaccgaag gacattgcag caccgttctg
     3841 agacttaaca gcagccaggt agccgaagta agtccactgg atagcttcct gagcgttggt
     3901 agccggacca gagatgtcgt agccgtattt cgcagccatt tctttcatct gacccagagc
     3961 gcggtgctgt tcagcgattt cttcgcgcag acggatagtc tgttccaagt ttacgccgtt
     4021 ttccagatca gcctgcagag aagtgaactg tgccagtttg tctttcatca ggtagtcgat
     4081 accgtacagc gcaacgcgac ggtagtcacc gatgatacgg ccacggccat atgcatctgg
     4141 cagaccggtc agaacaccag atttacggca acgcaggatg tccggagtgt aaacgtcgaa
     4201 cacgccctgg ttgtgagttt tacggtattc agtgaagatt tttttgatca tcggatccag
     4261 ttcgcggttg tacgctttgc aggaaccttc gatcattttg ataccaccga acgggataag
     4321 agcacgtttc agcggagctt cagtctgcag accaacgatt ttctcaagct gcttgttgat
     4381 gtagccagcg tcgtgagagg tgatggtgga agcaacagcg gtgtcaaagt caactggcgc
     4441 gtgagtgcgg ttttccagtt taacgccttc cattactttg tcccacaggg tggtggtcgc
     4501 ttcagtagcg ccagccagga aggactcgtc accctcgtac ggagtgtagt ttttctgaat
     4561 gaagtcacgg acgtttactt cattctgcca gtcacctttg gtaaaacctt cccaggctgt
     4621 ggctaacttt tcattaagct cggacatgta acacctacct tcttaagtgg attttttatt
     4681 tactgcgtac ttcgacaacc attaatggtg gtcgttttca cgcaggtaaa tgacccagta
     4741 tgtcaaccca accaacaaac caccaccgat aatgttgccg atcgtaaccg gaatcaggtt
     4801 atcagtgatg aaattcatca cggtcaggtg agaaaaattt tccggtgcag aaccgactgc
     4861 ggtccaaaat tccggggatg cgaagtcgcg gattacaata cccatcggga tcataaacat
     4921 gtttgcgata ctgtgctcaa aaccgctggc aacaaacatc gcgaccggca gcaccataat
     4981 gaacgctttg tccatcaggc tgcggccaga ataactcatc catactgcca gacataccat
     5041 caggtttgcc aggataccaa gacagacggc ctcaataaaa gtatggtgca ctttgtggtc
     5101 ggcggtttgt aggacgttta gtccccattg accatttgcg gtcatatact cgccggaaag
     5161 ccacattaaa agtacaaaca gcagtgcgcc gaccaggttg ccaaaataga catttagcca
     5221 gtttttcgcc aactgacccc aggtgatgcg cccactcgcc ttagcaacaa caatcaacac
     5281 ggtggaagta aagagatcgg ctccgcagac aacacaaaga atcagcccca gagagaagca
     5341 aatgccgcca accagttttg ccatgccgaa gggcattgtg cctgtgccag tggttgctgt
     5401 gatatagaag acgaatgcga ttgagatgaa aacaccggcg gtaatcgcca gatagaaagt
     5461 cttaagcgga tgtttcgttg ctttatagac acccgcctct tcggccactt tggccattgc
     5521 agcaggaagt aaaagatcaa aagggttgtc agctttcaca ctaactctct ctttattaag
     5581 tcggcgacga gatactaaca aagcattata gatgagaaat tgatatagat catatctcgc
     5641 ctggcttata ggcccgtaac tcgcatggtt tttatgca
//
LOCUS       DAZPDU010000232         5640 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZPDU010000232 DAZPDU010000000
VERSION     DAZPDU010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5640
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.235"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="ACHURS_004089"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="ACHURS_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFR1239694.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="ACHURS_004090"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="ACHURS_004090"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFR1239695.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="yfiH"
                     /locus_tag="ACHURS_004091"
     CDS             1882..2613
                     /gene="yfiH"
                     /locus_tag="ACHURS_004091"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFR1239696.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="ACHURS_004092"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="ACHURS_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFR1239697.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1401 a         1352 c         1529 g         1358 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agtggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctacg
      181 cggttaacga cggcaaccca tgcaccacgt tctgtatagt actcggcaac ggagtattca
      241 tatttcgcca gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgtgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc tcccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgga taaaagaatg
     1021 gatcctcgac cagcgcgtgc tggttaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaacgc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc ccgctggata tcgtctatga agatgaagat atcattgtca ttaataaacc
     1201 gcgcgaccta gtagtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt
     1261 gcttcattat tatccgccca ttgccgatgt accgcgtgcg ggcatcgtcc atcgtctgga
     1321 taaagacacc accggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctctggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc actgccaaaa
     1921 ggtgttgcgg cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgcaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgaggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcagcag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat tgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc ggcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcttggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga cgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgag tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaacattc tgttgcaggt actggatgat ggtcgtctga ctgacgggca ggggagaacg
     4861 gtcgacttcc gtaatacggt tgtcattatg acctctaacc tcggttccga tttgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggtaagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaagttaatg aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcggggct
     5341 cgtttttgtc tataagttag acggaaaaga ctatatttaa gatgttttgc ctgaaaaatg
     5401 agcgaacgat aaagttttta tatttttcgc ttgtcaggcc ggaataactc cctataatgc
     5461 gccaccactg acacggaaca acggcaaaca cgccgccggg tcggcggggt tctcctgaga
     5521 attccggcag agaaagcaaa aataaatgct tgactctgta gcgggaaagc gtattatgca
     5581 caccccgcgc cgctgagaaa aagcgaagcg gcactgctct ttaacaattt atcagacaat
//
LOCUS       DAZPDU010000233         5620 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZPDU010000233 DAZPDU010000000
VERSION     DAZPDU010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5620
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.236"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..513)
                     /locus_tag="ACHURS_004093"
     CDS             complement(<1..513)
                     /locus_tag="ACHURS_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFR1239698.1"
                     /translation="MGKYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIIDAELFALAGLDA
                     QHLQLLVHSGS"
     gene            complement(759..1211)
                     /locus_tag="ACHURS_004094"
     CDS             complement(759..1211)
                     /locus_tag="ACHURS_004094"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1239699.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVWVAVINAQPVGFISRIEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQHVECRGAWFTNFYMQYKPQH"
     gene            complement(1208..2263)
                     /gene="dinB"
                     /locus_tag="ACHURS_004095"
     CDS             complement(1208..2263)
                     /gene="dinB"
                     /locus_tag="ACHURS_004095"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFR1239700.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(2334..3119)
                     /gene="lafU"
                     /locus_tag="ACHURS_004096"
     CDS             complement(2334..3119)
                     /gene="lafU"
                     /locus_tag="ACHURS_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFR1239701.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSITTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            3091..4803
                     /locus_tag="ACHURS_004097"
                     /pseudo
     CDS             3091..4803
                     /locus_tag="ACHURS_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(4926..5423)
                     /gene="rayT"
                     /locus_tag="ACHURS_004098"
     CDS             complement(4926..5423)
                     /gene="rayT"
                     /locus_tag="ACHURS_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HFR1239702.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSQLLTTQYQMLRNAIIKVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDVTDINAG
                     ERIIL"
     gene            complement(5494..>5620)
                     /locus_tag="ACHURS_004099"
     CDS             complement(5494..>5620)
                     /locus_tag="ACHURS_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFR1239703.1"
                     /translation="HIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLEGSGLS"
BASE COUNT         1339 a         1468 c         1460 g         1353 t
ORIGIN      
        1 cgagccgcta tgaaccaaca gttgcagatg ttgtgcatcc agacctgcca gtgcaaacag
       61 ttcagcgtcg atgatttgat caacttgttg cagttcagcg aaatgattgc cgccaccgat
      121 agagccaagc gagctgcgcc acggatgctg tgcaaatgct gacggcaggt tttcctccag
      181 ccagctttct tcagcaacgt catccaaagc tgataatcgc ttttcaaact tatcggcgtt
      241 gtatttgcga gcgagaatat ctgtttgcca tagcgccatg ccgcagccga tatcattgcc
      301 gaccagtgct gggtaaaaac ggccaacaga gaagaacgct gcgccaatcg ggtagccgcg
      361 tccgggatgt aaatctggca tccccactac gcgctgcatg ttgggtaaat ttgccgtggt
      421 gtgtaattgt tggatcgcta aactctcgat ccacaggtca tcagatgcga tggtaaatac
      481 cgcatcggat aagggacgaa tatatttgcc catgtaccat tccgtaaata gactcgtcat
      541 acttcaagtt gcatgtgctg cgactcgaat tattgagagt aacaattaaa aaatcaggaa
      601 atggcagacg aatccagggc aagaaaaaaa gaattaatcc tggaaaggtc tgcaaagagt
      661 cattgataaa ggatttgtca tgatttacct ccttgcagtt tgtggaataa gaaaacgcgc
      721 acatactagg cgggcgttat tttcattgca agctggattt aatgttgcgg tttatattgc
      781 atataaaaat tagtaaacca cgctccccgg cattcaacgt gctgctgctt aactgtctga
      841 aaaccataac gttcaaaaaa gggttttgcg gttatgctgg catctaccgt aagttcggat
      901 tcagacttaa taaatggttt taacaaggcg ctggcaaccc cgcggcgggt gtattcaggg
      961 tcaacaaata acatatcgat ataatgttca atgcgggaaa taaaaccaac cggttgtgca
     1021 ttaatgaccg caacccacac ttgtgatttc gcgagtttct ccttccagcg agattcgtca
     1081 atctgcgccc aggcggcaat ttgttgtgga gaataatgct gactggcggt catcgtaacc
     1141 gctctaatga aaatagcgca tagttgctga aaatcgccag gctgatagtt tcttatttgt
     1201 atgttattca taatcccagc accagttgtc tttccatttg cggatcaagc aacgtcacat
     1261 gcagccccac cagtcgcaca ccgcgcccgc cgcggcgttc atcccaggtt ttacgcgcgg
     1321 tggcgattaa gtcagcttta ttcagccgcg gccagacgtg ctcctgagtg gtttgctgaa
     1381 aatcgtcgaa ctttaatttc accccctggc gggcaatcag taaatcaggt tttacctttg
     1441 ccagacggcg ttcaagttcc ggatacagcc gctcgataat cgcttcacat tcagaccagt
     1501 ggtggatatc ttccgccatc gtgcgttcca cgccgacgga ttttcgcaac cgttcgctgt
     1561 taacgtcgcg ctcgtcaatc ccctgactac gctcccacaa aatgcggcca aatttgccaa
     1621 agcgtttaag cagcatcacc agatcacact tttgtacatc accgcaggtt cgtagcccca
     1681 tcgcttccag ttttgccgcc gagactttgc cgacgccggg gatttttgcc agtggtaagg
     1741 tttgtaaaaa tgccggaact tctgccggcg taatcacaaa ctggccgttg ggtttattca
     1801 tgtcggaggc gattttggcg agaaacttta cgggtgccac gcccgcagac gccgtcagtt
     1861 gcagctcgtt gaaaatcgtc tggcggattt cctgggcgat gagggtcgca gaaccgtggc
     1921 aatggacgct atcggtgacg tcgagataag cctcatccag tgacaacggt tcaatacgcg
     1981 aggtgtagcg cgagaagatt tcgcggatat gatttgaggc ttctttgtag gcgtcaaagc
     2041 gccccggaag caaggtgaga tgcgggcata atttgagcgc catccctgtc ggcatagcgc
     2101 tacgtacgcc aaatttacgc gcgggataat tggcggtact gatcaccccc cgacgttcgc
     2161 ggctgccgcc aatagcaata gggatatcgc gcagggcggg attgtcgcgc atctccaccg
     2221 ctgcgaaaaa gcagtccata tccacatgaa tgattttacg cattgctcac ctctcaacac
     2281 tggtaaagta tacagtgatt tcagagtttg agaaatgcgt aaagattcag catttaacgc
     2341 gttcgctgcg aaagcacctg ctgcttatcc agcttttgca ccagcggctg caccacttta
     2401 tccccatgct gaccaaagta ttgatacagc gtatcggacg cacttttggt cagcaccata
     2461 atctcaatgc gccggttgcc cgcgctttgc ggatttttgg aatccagcag catctggtcc
     2521 gccattgcgc ttacctgcat cactttatct tccggcattc cggcctcttc cagcacccga
     2581 cgagccgaaa gcgcgcgatc gcccgaaagg ttccagttgt tgtagatatt gtttttgtaa
     2641 gccatcgcat cggtatgccc ggtaataata attttgttat cgagcgagtc gaaaactggc
     2701 gcgagctcca ccagcagcgt tttaaagaac ggcataatct gcgcgctgcc gcgttcgaac
     2761 atattgcgat tctggtcgtc tttaatcagc acgcgtaatc cctggggaac aatctccatc
     2821 tccagattcg cttccatatg cgcatcacgg gcaatggtgg tgatactggt tgccagctcg
     2881 ccaagctcgg tggcggattt cttcttcagc agcgccgttt tctcattgac gtcgcgcgct
     2941 tttttctccg tttcttccgg caccgccacg gtggcaggtt ttggcggatg agacgggctg
     3001 attttattta acggcgacag cccgccaccg ttaaaaatcg actgcccgtg cagcgcggcg
     3061 ataatgcttt cacgctcgga tttactgacg ctgttgacaa tcaactttat tgtcgtcacc
     3121 aaaggggccg agcgtatctc cgaggtttct gcccgcttta ccctggacgc gatgcccggt
     3181 aagcagatgg cgattgacgc cgatcttaat gccgggttga tcaatcaggc gcaggcacaa
     3241 acccgacgta aagatgttgc cagcgaggcc gatttctacg gcgcgatgga cggggcatcg
     3301 aaatttgtgc gcggggatgc tatcgccggg atgatgattc tggcgatcaa cctgatcggc
     3361 ggcgtctgta tcgggatctt caaatacaac ctgagcgccg acgccgcctt ccagcagtat
     3421 gtgctgatga ccatcggcga cgggctggtg gcgcaaatcc cttccctgct actctccacc
     3481 gcggcggcga ttatcgtcac ccgcgtcagc gacaacggcg atatcgccca tgacgtgcgt
     3541 aaccagttgc tggcaagccc atcggtgctc tacaccgcta ccggaattat gttcgtactg
     3601 gcggtagtgc cgggaatgcc acatctgccg tttttgctgt tcagcgccct gcttggcttt
     3661 accggctggc ggatgagcaa acgcccgcag gcggcggaag cggaagagaa aagtctcgaa
     3721 acgctgaccc gcaccatcac cgaaaccagc gagcaacagg tcagttggga aaccattccg
     3781 ctgatcgagc ccatcagctt aagcctcggc tacaagctgg tggcgctggt ggacaaagcc
     3841 cagggcaacc cgctcaccca gcgtattcgc ggcgtgcgac aggtgatatc cgacggcaac
     3901 ggcgtgctgc tgccggagat ccgcattcgg gaaaacttcc gccttaagcc cagccagtac
     3961 gctattttca tcaacggcat taaggctgat gaagcggata ttccggcgga taaactgatg
     4021 gcgctgccct ccagcgaaac ctacggcgag atagacggcg tgctggggaa cgacccggcg
     4081 tacgggatgc cggtgacctg gatccagcct gcgcagaaag cgaaggcgct gaatatgggg
     4141 tatcaggtga tcgacagcgc cagcgtgatt gctacccatg taaataagat tgtgcgcagc
     4201 tatattcctg atttatttaa ctatgacgat attacgcagt tgcataaccg tttgtcgtcg
     4261 atggcaccgc gcctggcgga ggatttaagc gcggtgctca attacagcca gttgctgaaa
     4321 gtgtaccggg cgctgctgac cgaaggcgtt tccctgcgcg atatcgtcac catcgccacc
     4381 gtgctggtcg ccagtagcgc ggtgaccaaa gatcatattc tgctggcagc cgatgtgcgc
     4441 ctggcgctgc ggcgcagcat tacccatccg ttcgttcgca agcaggagct gacggtgtat
     4501 accctgaata atgagctgga aaatctgctg actaacctag tgaatcaggc gcaacagggc
     4561 ggtaaagtga tgctcgacag cgtgccagtg gacccgaata tgctcaacca gttccagagc
     4621 acgatgccac aggtgaaaga gcagatgaaa gcggcgggga aagacccggt gctgctggtg
     4681 ccaccgcagc tgcgcccttt gctggcgcgt tatgcaaggt tgtttgcgcc ggggctgcat
     4741 gtgctgtcgt ataacgaagt gccggacgag ctggagttga agattatggg ggcgttgatg
     4801 taagtatccg gcattggtgc acaactgccg gatgcggcgt aaacgcctta tccggcctac
     4861 cgttcaggca caagtttgta ggcatgataa gacgcgccag cgtcgcatca ggcatctgcg
     4921 cataattata aaatgatacg ctcgccagca tttatatccg ttacgtcccc cgcccaatcg
     4981 atgggatata accctcgcgc gacatcgcga tggaacgttg agaatggcca atcactcact
     5041 tgctttaccc aaccatgctt tactggattt atataaatat aatcaacatg atgcctataa
     5101 tctttggtat tgcggatggc gtgctcccaa aaacgtggtt gccagatatt tttcaatcca
     5161 caagcatggg taaattgctt tttaatttcc cgccagcgag aggaaaaatc atcatcgcct
     5221 tcaggtaatg tccatatgca atgcatatgc tctggcaaaa cgacccaggc gttgatttca
     5281 aaaggcctgt ctcgcttaac tttaataatg gcgttacgga gcatctggta ctgggtggtc
     5341 aagagttggc ttcgacgatt gcgtaaattc accgtgaaaa accaggttcc ccccttgatg
     5401 taataacgac gatattcaga catggcgact cctttttctc aacggtaaga agaaaaggaa
     5461 aggtcggcaa tgaggtgatg gaacagggca agattacgaa agcccgctcc cctcaaggac
     5521 tgacgccaga tagtttctgt ccatggctgc ttttcgcatc ttacgtctta accctgcctt
     5581 gaatacctta tcattcgtca aaatattaat agcgatgtgc
//
LOCUS       DAZPDU010000234         5613 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZPDU010000234 DAZPDU010000000
VERSION     DAZPDU010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.237"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(104..418)
                     /locus_tag="ACHURS_004100"
     CDS             complement(104..418)
                     /locus_tag="ACHURS_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HFR1239704.1"
                     /translation="MTQNYELIVKGIRNFENKVTVTLALRDKKRFDGEIFDLDISLDR
                     VEGAALEFYEAAARRSIRQVFLDVAAGLCEGDELLPETRPCSEARYTIKINSSDNSIT
                     GC"
     gene            complement(423..638)
                     /locus_tag="ACHURS_004101"
     CDS             complement(423..638)
                     /locus_tag="ACHURS_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFR1239705.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            complement(789..2642)
                     /locus_tag="ACHURS_004102"
     CDS             complement(789..2642)
                     /locus_tag="ACHURS_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFR1239706.1"
                     /translation="MAFKHYDVVRAASPSDLAKRLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPEWYYVIVLAGQSNAMAYGEGLPLPDSYDAPHPRIKQLARRN
                     TVTPGGEVCVFNDIIPADHCLHDVQDMSTINHPRADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCCGGSAFTQGTEGTFSESTGASQDSARWGVGKPLYQDLLFRTKAAL
                     QKNPKNVLLAICWMQGEFDMTNASYAQQPAAFLAMVQQFRADLAGLAAQCHGGSPASV
                     PWICGDTTYAWKQEHGTQYEVVYGAYKGKESQQIYFVPFMTDGSGVNTPTNNPSEDPD
                     IAGSGYYGSASRTNKNWVSSNRPTHFSSWARRGIIPDRMATAILNVAGRTLAFISGKA
                     PEIKPSPGGDTPSGPSEDASIRTISLLPTAGDAAAQGWSIKNGGVQLSDGVFKITKQS
                     NKAWSLTRPVDDAVSLLTRGGRLSCKFRLSGALTNNQFGLGIYLYTDVALPDVVAMTG
                     TGNPFLMSFFTQTTDGKLNLMHHKKAGNTKLGEFGNYSNDWLTLELVFTAGSATVTPK
                     LNGVAGPAFQVIKDSLTLGLNALTLTDITKNAAYGVEIESLVLEINAPASS"
     gene            3397..3513
                     /locus_tag="ACHURS_004103"
                     /pseudo
     CDS             3397..3513
                     /locus_tag="ACHURS_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3618..3694)
                     /locus_tag="ACHURS_004104"
     tRNA            complement(3618..3694)
                     /locus_tag="ACHURS_004104"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3658..3660),aa:Arg,seq:tct)
     gene            complement(3708..3784)
                     /locus_tag="ACHURS_004105"
     tRNA            complement(3708..3784)
                     /locus_tag="ACHURS_004105"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3748..3750),aa:Arg,seq:tcg)
     gene            complement(3792..3867)
                     /locus_tag="ACHURS_004106"
     tRNA            complement(3792..3867)
                     /locus_tag="ACHURS_004106"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3832..3834),aa:Ile,seq:cat)
     gene            complement(3908..4966)
                     /locus_tag="ACHURS_004107"
     CDS             complement(3908..4966)
                     /locus_tag="ACHURS_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFR1239707.1"
                     /translation="MLNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYAAKGSALKQHVMA
                     PLISYFRDARAALGITAKQIADATGKKNMVSHWFSAGQWQLPNESDYLKLQALFARVA
                     EEKHRRGELEKLHHQLVDTYTSLNRQYAELLSEYKHLRRYFGVTVQVPYTDVWTHKPV
                     QFYPGKHPCEKPAEMLQQIISASSRPGDLIADFFMGLGSTVKAALALGRRAIGVELET
                     ERFEQTVREVQDLVSQNG"
     gene            complement(5074..5314)
                     /locus_tag="ACHURS_004108"
                     /pseudo
     CDS             complement(5074..5314)
                     /locus_tag="ACHURS_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309113.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5539..>5613)
                     /locus_tag="ACHURS_004109"
     CDS             complement(5539..>5613)
                     /locus_tag="ACHURS_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR1239708.1"
                     /translation="YDALVTRFDIEEAWAERQLKKVTR"
BASE COUNT         1338 a         1583 c         1321 g         1371 t
ORIGIN      
        1 cagcgccaga acagcagctg acaggccgta tttgattttt gcgctcatgg atatttatca
       61 ggatgctacc aatgaaagat actggaaagc caactgcaaa aagctaacaa cccgtaatcg
      121 agttatcaga actgttaatt tttatggtat accgcgcctc tgaacagggg cgcgtttctg
      181 gcaacagctc gtccccttca cataacccgg cagcaacatc caagaagacc tgtctgatgc
      241 tccttctggc tgctgcctca taaaactcca gcgcggcacc ttcaacacgg tccagcgaga
      301 tgtccaggtc aaaaatttca ccgtcaaagc gttttttgtc ccgtaacgct aaagttaccg
      361 taactttatt ctcaaaattg cggatccctt tcacaatcag ttcatagttt tgagtcattg
      421 aattactctc cccgtgcagc cttacgacgg tcctctctga ttttgaaata caggttagtc
      481 agatatgtca gcagcccaaa cagcagactc cccagcacgc ctattgccgc ccactgagac
      541 ggggaaaccc tgtccagcaa ctgcaggaac cagtagcccg ttcccaccgc tgacgtggtg
      601 tatgacacac ctgttgtgat tttttccatc tggtacatac cccgtctccc gttatccgga
      661 agctgacaac aataaaaaaa gccaccagtt aagtactgat ggctctgata actcatgcag
      721 gcatctcaga cgacccactg acactaccgg tgagtttaac gataccttcc atttggctgg
      781 ctcacttttt atgatgatgc cggtgcattt atctccagca ccagactttc tatctcaacg
      841 ccatacgctg catttttggt aatatccgtc agcgtcagcg cattcagccc cagtgtcaga
      901 ctgtctttta tgacctggaa tgccgggcca gccactccat tcagtttcgg agtaaccgtg
      961 gcactgccgg cggtgaacac cagctccagc gtcagccagt cgttactgta attcccgaac
     1021 tcgcccaact ttgtgtttcc tgctttcttg tgatgcatca gattcagttt gccgtctgtg
     1081 gtctgggtga agaacgacat caggaacggg ttaccagtcc cggtcatcgc cacgacgtca
     1141 ggtaacgcta catcggtata cagataaatt cccagaccga actggttgtt ggtcagtgcg
     1201 cctgacagtc gaaacttaca gctcagtctg ccaccccgtg tcagcaggga gactgcgtca
     1261 tccaccgggc gcgtcaggga ccaggcttta ttgctctgct tggtgatctt aaatacacca
     1321 tctgacaact gaactccgcc attcttaatg ctccagccct gcgcagcagc gtctccggct
     1381 gtcggcaaca gggagattgt gcgtatggat gcatcttcag acggccccga tggcgtgtcg
     1441 ccgccgggcg agggtttgat ttccggtgcc ttaccactaa tgaaggctaa ggtgcgaccg
     1501 gctacgttca gaatagcagt tgccatacga tcgggaataa tgccacgacg cgcccatgag
     1561 ctgaaatgcg tcgggcgatt tgatgatacc cagtttttgt tcgttcggga tgccgaaccg
     1621 taataaccag acccggcaat atcaggatct tctgacgggt tgtttgtcgg tgtattaact
     1681 ccgctaccat cggtcataaa gggaacaaaa taaatctgct gggattcttt acctttatat
     1741 gcaccatata ccacttcata ttgcgtaccg tgttcttgtt tccacgcgta tgtcgtgtcg
     1801 ccacaaatcc aggggactga tgccggactt ccaccgtgac actgcgccgc cagcccggca
     1861 aggtcagcac ggaactgctg taccattgca agaaatgctg ctggctgctg ggcgtaactg
     1921 gcattcgtca tatcgaattc cccctgcatc cagcatatcg ccagcaaaac gtttttcggg
     1981 tttttctgca atgctgcctt cgtgcggaaa agcagatcct gatataacgg cttacccact
     2041 ccccagcgag ccgaatcctg actggccccc gtggactcgc tgaatgtccc ctccgtgccc
     2101 tgggtgaatg ccgaaccacc acaacagcat ggtaccagca ggatccccgc attattaggg
     2161 atatacggaa gcagtttttt ggcaatatgt aagccctgtc cgacacagcc gtactgccct
     2221 ttgctcaggt cagcccgggg atggttaatc gtactcatat cctgaacatc atgcagacaa
     2281 tggtcagcag gaatgatgtc gttaaatacg catacttcac caccgggagt cactgtgtta
     2341 cgacgggcca gttgcttaat gcgcggatgg ggcgcatcgt atgaatccgg aagcggaaga
     2401 ccttcaccgt aagccatggc attggattgc ccggccagta cgatgacgta gtaccactcc
     2461 ggctcagttg caccactgac gaccacatca ccttctgctg caatcgcctg catcagggta
     2521 taaggggtta tggccaccgg actaccaaac ggctgccagc cctccttcag tttttgtgtc
     2581 agtcgtttcg caaggtctga cggcgatgcc gccctgacca cgtcatagtg tttaaatgcc
     2641 atgaatcctc ccggccggga taatattgtg agtaaaataa ggagcgggct gaagtccgga
     2701 agttacagga caatggcaga agagagacga cagcccgcaa ttcgaaaaag accgcgcagt
     2761 tgcgcagagt gattactatg gggtattatt cgccagctga aatattactt cacgttttat
     2821 tgtttattcc ttgccgcccg cgtctcccag cgcgggcttt ttttgcccac aagaaaaccc
     2881 ctccggagag gggctaaagc cgcgtatctg tatcatcatg cacatggtgc cgggtgcctc
     2941 ccggtgagtt cagcccggtg ccactaaacc cgcgtcattc tcgttttgat aatcagagat
     3001 tataccgtca ccagtcgccc ctccgctcag ggggattcac catgcgaaat ttttttaaca
     3061 aatgcccagt ctgacaggca actgtcaact tactgaattg tgagcaacat agcatttaac
     3121 ggggaacctg ttttctgcag taaaaaggcc caccggagcg gatgggcctg gaaggatagc
     3181 ggtcatgtga tgccggtttc ccggtaactc agcaccggta tctgagtcaa cgttttctct
     3241 actgggtcat ttccgatacg ccctgcctgc tgacaggctt tcatcacatc tgaaaatata
     3301 gcaccctgac tgatactgta gtacctaagg ttccagaaac tgtgatgtat ccggcacaga
     3361 aaagcccctc cggagagggc tggagagtgg cgctatgtgc cattgcatga tgccgggtgc
     3421 ctcccggtga attcagtacc agcacctgaa tccgcgatta tcccatatac ctactcgctg
     3481 attgcccctc cgcacagggg gattcaccat gccagtttct tttaacaaac tccccgcaaa
     3541 acagacaact gtcaaccgtc tgaattgtga gacatttaaa aaaaaggccc gcaaaagcga
     3601 gccagggaaa ataagtgtgg cgcgttgtac tggattcgaa ccagtgaccg attgcttaga
     3661 aggcaattgc tctgtccggc tgagctaaca acgcatgatg cagataatgg accgccatcg
     3721 gggacttgaa ccccgcgcag ccagcttcga aggctggcgc tctttcctga tgagctaatg
     3781 gcggtatgtg atggtggccc ttgctggatt tgaaccagcg acctggcgat tatgagtcgc
     3841 tcgctctcac cactgagcta aagggccggg agccgcataa taacgacgcg taattaattc
     3901 ttcaatatca tccgttctgg ctgactaaat cctgtacttc ccgaaccgtc tgctcaaaac
     3961 gttcagtctc cagctcaacg ccaattgcac gacgcccgag cgccagtgcc gctttcactg
     4021 tcgaacccaa ccccatgaaa aaatctgcaa tcaggtcacc cggacgactg ctagcgctga
     4081 ttatctgctg cagcatttct gccggttttt cgcacggatg tttcccggga tagaactgca
     4141 ccggtttatg cgtccacaca tcggtatacg gcacctgcac cgtcacgcca aaataccgcc
     4201 gcagatgttt atattcactc agcagctccg catactgccg gttcagtgag gtatacgtat
     4261 ccaccagctg gtggtggagc ttttccagtt caccacgccg atgcttctct tctgccaccc
     4321 gggcaaacag cgcctgtaat ttcagataat cgctttcatt cggcaactgc cactgaccgg
     4381 cactgaacca gtgcgacacc atgtttttct ttcctgtggc atctgcaatc tgttttgccg
     4441 ttatccccag ggcagctcgc gcatcacgaa agtaagaaat cagcggggcc atcacatgct
     4501 gtttcagtgc actgcccttc gccgcatacc cggcatcttt cggacgatac ggcccctgat
     4561 aatgttccgc gaacagaatg cgctctgtgg cggggaaata cgcccgcagg ctttccttgt
     4621 tgcatccgtt ccagcgtccg gacggcttcg cccagataat atggttcagc acactgaagc
     4681 gttcacgcat catgatttcg atatcagatg ccaggcgatg accacagaac aggtaaagac
     4741 ttccggcagg tttcagcacc cgccagaact gcgccagaca ctggtccagc cacttcaggt
     4801 aatcatcgtc gcccttccac tggttatccc agccctcagg cttcacttta aagtacggcg
     4861 ggtccgtgac tatcaggtca acagaatttt cgggtaacga ccggataaat tccaggcagt
     4921 cggcgttgat taactcacaa ctggatattt ttacagtatt aagcatggat cattaagcct
     4981 gtctctgata ggctcattct gcttttgcgc aaagcagtgg gcctgaggtt tgcttgtgaa
     5041 cccaacgcat gagcagatgg ctggtgggtg cccctaacac ccaccagccg cccatttacc
     5101 acaaataaaa aagccttcac tgcggaaggc gtctgtaaca accgaactga tagtctgcca
     5161 gacccgccat aaccagctgg gtcagtatta actggcagcg ttcgcgtgaa aggtaagtat
     5221 tctgcgctat ctccccgact gtcgccggtt cggtaacgct taattcatta aacaccactc
     5281 tggcggtttc tgtcatatcc tgctgtttta gcatgtcttt tccttttccg gttaacgtga
     5341 cacaccaata actcttgtcg aaaaagccag caagctgaaa gacaggtatt caccgccacc
     5401 agcgcgttta ctgtactgac gcgatttcag tcataaaaaa cccgctcggc ggcgggttgt
     5461 agaaactctt ctaacgtcag gcataaaacg cccatcgtta tgacgaattt accacagatt
     5521 ccggaaaaat caaccttgtt acctagttac cttttttaac tgccgctcag cccatgcttc
     5581 ttcaatatca aaccgggtca ccagcgcatc ata
//
LOCUS       DAZPDU010000235         5558 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZPDU010000235 DAZPDU010000000
VERSION     DAZPDU010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5558
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.238"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(78..197)
                     /locus_tag="ACHURS_004110"
                     /pseudo
     CDS             complement(78..197)
                     /locus_tag="ACHURS_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
     gene            complement(302..1804)
                     /gene="dtpA"
                     /locus_tag="ACHURS_004111"
     CDS             complement(302..1804)
                     /gene="dtpA"
                     /locus_tag="ACHURS_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFR1239709.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(2416..3051)
                     /gene="nth"
                     /locus_tag="ACHURS_004112"
     CDS             complement(2416..3051)
                     /gene="nth"
                     /locus_tag="ACHURS_004112"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFR1239710.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(3051..3746)
                     /gene="rsxE"
                     /locus_tag="ACHURS_004113"
     CDS             complement(3051..3746)
                     /gene="rsxE"
                     /locus_tag="ACHURS_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFR1239711.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(3750..4370)
                     /gene="rsxG"
                     /locus_tag="ACHURS_004114"
     CDS             complement(3750..4370)
                     /gene="rsxG"
                     /locus_tag="ACHURS_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFR1239712.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNTLAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(4374..5432)
                     /gene="rsxD"
                     /locus_tag="ACHURS_004115"
     CDS             complement(4374..5432)
                     /gene="rsxD"
                     /locus_tag="ACHURS_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFR1239713.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSTLLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(5433..>5558)
                     /locus_tag="ACHURS_004116"
     CDS             complement(5433..>5558)
                     /locus_tag="ACHURS_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transporter RnfC"
                     /protein_id="HFR1239714.1"
                     /translation="EQQQTNAEPEEQVDPRKAAVAAAIARAQAKKAAQQKVVNED"
BASE COUNT         1464 a         1410 c         1365 g         1319 t
ORIGIN      
        1 ccggaaacaa gtgagcgttt ccggattctt acacagccac tggatcggtc aactgatcct
       61 taactgatcg gcattacacc gttttcgaga cgttttttca ttaaatccac actgacgagg
      121 gtaaaatcct ttccgctctc acgcagggtg atatgggaag cgagagagca ggcacccggt
      181 ttgtagaaca atttcatcgg taactccttt tgtctgtggt ttaaagtatg gtagtgcgtt
      241 gctgaacaaa aaaaagccgc taacgaaaag ttagcggctt gtaaaagagt ttccctgaat
      301 tttacgctac agcagctttc gccgctttgt ctgcagcgtc atcctgcgtc atacggtgca
      361 gtttaggcgc ggtcagcagc atcagtactg caataatggc agtagcgaca ccaatctgca
      421 agaatacgcg accataaact tccagtgaca tcagcgggtc ggtaacgtta tccggcacag
      481 ccatcatacc cgcaacataa ccaccaatca ggtttgcacc ggcagtggtc aggaaccagc
      541 tacccataat gaagcccatc agacgctgcg gaacgagttg tgcaaccatt gccagaccca
      601 gaccagagat catcagttcc ccgatgctct gcaggccata gcttgcgacc agccagctta
      661 cagacacgat accagcgtca gacgcgaatt tcgcacccag cggcagaatc aggaacgcac
      721 cagaacacat caccatgccg attgcaaact tggttggcat cggcagggta tcgcccatct
      781 tgttatagat agcggccaga atcggactac cgatgatgat ccagaacggg ttcagtgcct
      841 gatactgctc aggttctacg gccagaccca gaatggagtg ctcaacgtta cgaatcgcaa
      901 agaagttcag tgacgttggc atctggctgt acagcacgaa gaagataatg gcttcgagca
      961 tcaggatgaa ggcaacgatc attttacgac gcgcagcacc tttcatcgcg aaggcttctt
     1021 taccgaagat aaccacgata ccgaaggcaa caacgcccag cgccatacgc gcaacttcct
     1081 gattgtgcag cagccaggtg gcgatagcga tcagtgccac aacaccaata atggtcagca
     1141 gcaggttacg gtagttgata ggctcgaagt ctggttttga accgtactgt ttaacccagc
     1201 gttggcagaa ggcgaagtta acgatagtga tcagcaggcc tacaacgctc aacgcaaacg
     1261 caacactcca gccgtatttc gcggccagcc acggcgtagc aatcatagag aagaaagagc
     1321 cgatgttgac ggacatgtag tacatggtga atgcaccgtc cagacgcggg tcgtttttct
     1381 catagcatgt agaaagcaga gaagatgggt tagctttaaa caggccgtta ccgaccgcaa
     1441 tagccgccat acccatataa acgataccgg cgtcgtgacc agaccaggca accagagcat
     1501 aaccaatcgc cagcacaata gcgccgagca taattacgcg tttagtaccc agtaccttgt
     1561 cacctaacca gccgccgata gcgaccagac cataaaccag ggcactaaag gaagagaaaa
     1621 gggtgattga atccgcttca gacataccca gttgtttaac caggtaaaca gccataattc
     1681 cttgtaggcc gtaataacca aaacgttccc ataactcaat cgagaagatg agatagaacg
     1741 ccttcggttg tttgaaagcg ttcaaactga cgctttcagt tggtttttgg tttgcagtgg
     1801 acacttttac ctcttttttt acatcccata ttaacggggg ggctttctcg cgttatgtct
     1861 aaaaaacata cgcgcaattg ttatagtggg aggggaaacg gcaggtaatg ttcactatct
     1921 tgcactttca gacaagtcct ttgtaataat ctgttacata taactgaggc ggggaattca
     1981 accattccgg gaaatgttat ccagcgttaa atttttcaaa aaacgtaatg gcagattttt
     2041 tcactgcact gagagggggg gtgatggctt aacggcgata tgttctgcta tttggcgttt
     2101 ttactagtga gatagtccag tttctgaaaa atagccagtg taatgttttg taggtcaata
     2161 tttgttgcgt gtgtaaattt aaattatttt aacctgattc ttgcaatgtt tttacattgc
     2221 gacagcatcg tgatcttgat cacgaatgaa tagaaaattt cgctattaaa aaaacaatta
     2281 acctgcattt ttggttatcc gcaaacggat tacctgatga taacgctaag caatggcatc
     2341 attcacgtta aaaagatgtt ttaaaagagg ataaagaaag gttatcaatg gggtaatcgg
     2401 tgttacccct tttcttcaga tgtcaacttt ctctttgtat tcacaaagat cttcaataat
     2461 acaagagcca cagcggggct tgcgggcaat gcaggtataa cgcccgtgca ggatcaacca
     2521 atggtggcag tcgactttaa actctgccgg aaccactttc agtagctttt cttctacctg
     2581 ttcgacgttt ttccccggcg caaattgagt acgattacaa acgcggaaaa tgtgcgtgtc
     2641 gacagcaata gtcggccagc cgaatgcagt gtttaatacg acgttggctg ttttacgacc
     2701 tacgccgggc agggcttcaa gcgcagcacg atcttccgga acctcgccat tatgctgctc
     2761 cagtaagata cggcaggttt tgatgatatt ttctgctttg ctgttataaa gcccaatcgt
     2821 tttgatatag gttttaaccc cttcaacgcc cagttcaagc atcgctgcag gcgtattcgc
     2881 caccgggtaa agtttcgccg tcgccttatt aacactgaca tcggtcgcct gagcggaaag
     2941 cagtacggca atcagcaatt caaaaggcga actgaaatta agctcggtgg tgggatgagg
     3001 attgttctca cgcaggcgag tgaggatctc caggcgtttt gctttgttca tcagacattc
     3061 cctgtttcac cgtttggcaa tgcacgttcg gcagctgctt cagtacggcg ctttttcatt
     3121 ctttcatcaa tcaggtattt tcctgccagc atcagtccca ggccaataaa tgcacctggt
     3181 ggcagcatcg ccagcaggaa aggggagtcg gtgtggaaaa tctccacgcg taatactttt
     3241 gcccagctac ctaacagcgc atctgcaccg tcaaacaatg tgccattgcc gataatttcg
     3301 cgtagtgaac ccagcacgaa catggcgcag gttgcgccca taccaattga aaagccgtcc
     3361 agtgccgaaa gcgccggacc ttttttggcg gcgaaggctt cagcgcggcc cacaacgata
     3421 cagttagtga caatcagcgg aataaaaatc cctaatgatt gatacaggcc aaaggcgtag
     3481 gcgttgatca gcatctgtac agcgctgacc accgaggcga tgatcatcac gtaaatggga
     3541 atgcggatct cggctggcgt ccagtgacgc agcgtcgaaa tggtcaggtt ggtcagcgtc
     3601 agtaccagcg tagtcgcaag tcctaaaccc agagcgttag tggcagtgga cgtgaccgcc
     3661 aacagaggac aaaggccgag caactggacc agcgcagagt tgtttttcca caacccctga
     3721 acaataacgt ctttaatttc gctcacgctt tattctccac aggcaggaag ttgagaaagt
     3781 tgtgccggta acgtctgagc gtacaatccg gcgcgtttta ccgcattaac caccgcgcgg
     3841 ggagtaatcg tcgcgccggt gaactggtcg aaatcaccac catctttctt caccgcccag
     3901 tgcgcatcat ccgcaccact gattttttta cccgcaaaat gggtgatcca gtcagaaagg
     3961 cgcagttcga ttttatcgcc aagccctggc gtttcgtggt gctctgtcac gcgcgtgcca
     4021 agtaccgtgc cgttaaaatc ggctcccacc agcagctgaa tcgcaccgga atagccatct
     4081 ggcgcggttg cttccagaac ggcggctacc ggtttgtcat cctgtttggc gatgtaaacc
     4141 cgatgctcac ctttacctaa ctctggcgca gttaccagat agcaactctg tgccagcgta
     4201 ttgttatagc gttcggctgg cagcacctga tcaaataatg ccttttgttg cagactggcc
     4261 tgttcagcaa tcgtcgtttt ggtcatctgg ttgatggccg cagttaaccc tgttgaaccc
     4321 gctgcaaata gcgctaacgt aatgccgtgt tttcggatag ttttcagcat ggtttaccct
     4381 ttgcgatggc cgtagacgcg cggacgcgtg tagtaatcga tcagaggaac cgtgatgttc
     4441 gccagcagga cggcaaaagc cacgccgtca ggatagccgc cgaaactgcg gatcaaccag
     4501 actaataagc ccgcaagcgc gccgaaaata agacgaccac gattggtcgt agaagcggta
     4561 accgggtcag tcaaaataaa gaatgcgccg agcatggtcg caccagacag cagatgaatt
     4621 tgcggtgctg ccagtgtttc tggtgagaac aaccagccca acgttgcgca taacgccagc
     4681 gttactaaga agctgagggg aatatgccag cgaatcgctt tctgccatag caaccacagg
     4741 ccgccagcca gccaggcgag atttacccat tgccaaccag cgcccgccag aataccgctg
     4801 tagatcggat attgcataat ctgttcaacc gaatgaccgg cacggacaga ggttttaaat
     4861 gtatccagcg gtgtcgcctg actaatgcca tcaataccta agcgtagtgt gttcatatca
     4921 ccaccactgg cggtatgccc gctgaaaata acctgtatgg cgtcgataaa accagggatg
     4981 ttgaccgcaa tttcatgtgg cggtaaccag ctggtcatct gcaccgggaa ggagatcagt
     5041 aagaccacat aaccaatcat tgccggatta aacgggtttt gccccagacc gccatacaac
     5101 tgtttagcga tgatcaccgc aaacaccgta cccagcacga ccatccacca tggcgcgagg
     5161 gggggaatac ttaccgccag caataagcct gtcagcaatg ctgagttatc tttcaacgtt
     5221 gcggctaccg actgcttgcg tagtttgagt acgagagctt cggctaacag agtactaacc
     5281 gatgccaaca ggatctgaac gagagtaccc caaccaaaaa accacagttg cgctgcgatt
     5341 cctggcacgg ctgcgagcaa caccaacagc ataatgcgcg atgtctggcg ctggttatgg
     5401 gtataagggg agctagctat tctgaatacc atttagtcct cgtttacaac cttctgctgg
     5461 gcggcttttt tggcctgagc gcgggcaata gccgcggcaa ctgccgcttt gcgcggatca
     5521 acctgttctt ctggttctgc gttagtctgt tgctgttc
//
LOCUS       DAZPDU010000236         5541 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZPDU010000236 DAZPDU010000000
VERSION     DAZPDU010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5541
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.239"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..1369)
                     /locus_tag="ACHURS_004117"
     CDS             complement(<1..1369)
                     /locus_tag="ACHURS_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFR1239715.1"
                     /translation="MKITTVDVCIICGIFPLLILPQLPGTLALAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITYLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAVLLAAGLIRSGQIFLPGRWIHWQIPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWL"
     gene            complement(1576..1860)
                     /gene="ihfB"
                     /locus_tag="ACHURS_004118"
     CDS             complement(1576..1860)
                     /gene="ihfB"
                     /locus_tag="ACHURS_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFR1239716.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(2020..3693)
                     /gene="rpsA"
                     /locus_tag="ACHURS_004119"
     CDS             complement(2020..3693)
                     /gene="rpsA"
                     /locus_tag="ACHURS_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFR1239717.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(3804..4487)
                     /gene="cmk"
                     /locus_tag="ACHURS_004120"
     CDS             complement(3804..4487)
                     /gene="cmk"
                     /locus_tag="ACHURS_004120"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFR1239718.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(4660..5424)
                     /gene="ycaL"
                     /locus_tag="ACHURS_004121"
     CDS             complement(4660..5424)
                     /gene="ycaL"
                     /locus_tag="ACHURS_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFR1239719.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         1410 a         1450 c         1304 g         1377 t
ORIGIN      
        1 atagccattg ccaacgttca gcaatgttga tccacccttc tggcaaactc tttaaccccc
       61 agaaaagtaa agccaaagac cggtcggcaa gaaaccataa cccttgctcc aggattaacg
      121 gcccgcttaa atgcacaacc atcgcggcga ggatcaacgg aaccgtgata aatgtcacca
      181 agggaattgc taatagattt gcaataaacg aggtcagact aatgccatga aatatgacga
      241 tttgcacggg cataagcaga agtgtgatac ccagttgcag atggatgagg gaaacaactg
      301 cacgcaatac cggcggcagt tgccactcag gacagggaaa ccactgatac caaaaaatca
      361 gtgccgcgac cgcagcggca gagagccata aactttgcga gagaatggca acaggatcca
      421 tcagcaaaat tgccgccaga caacatatcc atacatccca gccactccac tgtcgcccac
      481 ttaacttaag cattccccac gtagcaagcg ccaccacggt acgcaatgca ggaggttgca
      541 ttcccgtcaa ccaggcataa aaagcagcac agcagatccc accaattaat ggtatttgcc
      601 agtggatcca gcgcccaggc agaaaaattt gtccactgcg aatgagtccg gcagccagca
      661 acaccgcaaa agcgatgtgc aatcccgata tcgccattaa atgcgccgtt ccagtatcgc
      721 gcatgatatt tttgatttct ttggggacgg ataaccgttc ccccatacct aaaccaagaa
      781 taaccgcatt ccacggatag ggttgcagcg ttgtttgtag tgacgccaga tactgtgcac
      841 gcaggctaca attcggttca ataacacttg cctgcagaaa gcggccggtg agcggctgat
      901 gctgggcaat ggcataacgc tggctatcaa agccgccatc attaagttga ccatgaacgg
      961 cacgaacttt gagtttcatt gaccacagtt gtccggcaca aaccgcttgc ggaagatatt
     1021 cgccatacag cacaaggcct ggcgcaggga atatacgtcg accttgtaga taagtaattt
     1081 gaccgtaatg agtggtcatg ccgtcagtag cagtgatctc aacaattgca tcctgcgtcg
     1141 cgccagttaa ggtttctcct gcccacaaaa tttgctttgc tgataatatg ccccaaacga
     1201 aaaacagcaa cgtcagcgcg atataacgga cggttttaac aggtataaat gccagtacgc
     1261 aggcgaagag agtcagaaac gcaagggcta atgtcccagg caattggggc aaaatcagca
     1321 acggaaaaat tccgcaaatt atgcatacgt cgactgtcgt tattttcatg cactcctccc
     1381 tgtttatgga aagtgtgcaa ctttgttgag cggttgtctg gtgggaattt tgcgttcccc
     1441 gaggcacatt cagaagaatt taacgagagg gtgaaaactg ctcaaaactc tgcgaggcag
     1501 cttccagttc ctgattagag aaatgaaaaa agcacccgac aggtgctttt ctcgcgttca
     1561 agtttgagta aaaacttaac cgtaaatatt ggcgcgatcg cgcagttctt taccaggttt
     1621 aaagtgagga acgtattttc cttccagttc tactttatcg ccagtcttcg gattacgtcc
     1681 ggtacgtggt gcgcggtagt gcaaagagaa actgccgaaa ccgcggattt caatacgctc
     1741 gccctgcgca agagtcgagg ccatatgctc cagcatctct tttactgcat cttcaaccgt
     1801 cttggcggga atgtgcgatt gctgggtggc aagtctttct atcaattctg acttggtcat
     1861 gattcctccg gttccttaaa ggcaaattaa gcggctgctg ttgctgcatt gattaagggc
     1921 ggccgtagcc gcccttagtg cttgattaca ggacgaaacc tgcaatctgt caagtaaact
     1981 caacaaactt cggaataaaa atcccgaaga gtcagagaat tactcgcctt tagctgcttt
     2041 gaaagcttca gccattgcgt tgttggagaa gtttgcatct tcctgtttgt taacagttgc
     2101 gattgcatct ttctcgtcag cttcgtcttt cgcacgaaca gacaggctga ttgcgcggtt
     2161 tttacgatca acgccggtga atttagcttc aacttcgtcg ccaacgctca gaaccaaggt
     2221 agcgtcttca acgcggtcac gagatgcttc agaagcacgc aggtaacctt caacgccgtc
     2281 agccagttct acggttgcgc ctttagcgtc aactgcagtt actttaccgg ttacgatagc
     2341 gcctttcttg ttcagagcaa cccagttgtt gaacggatct tctgcgagct gtttaacgcc
     2401 cagggagata cgttcacgtt ctgcgtcaac ctgcagaaca actgcagcga tttcgtcgcc
     2461 ttttttgtat tcacgaactg cttcttcgcc tgcaacgttc caggagatgt cagacaggtg
     2521 aaccaggccg tcgatgccgc cgtccaggcc gatgaagata ccgaagtcag tgatagactt
     2581 gattttacct tcaacacggt cgcccttgtt gtgggtttcc gcgaactgct gccacgggtt
     2641 agctttgcac tgtttcagac ccagggagat acgacgacgt tcttcgtcga tatccagaac
     2701 cataacttcc actacatcgc caacgttaac aactttggac gggtggatgt ttttgttggt
     2761 ccaatccatt tcggaaacgt gtaccagacc ttcaacgcct tcttcgattt caacgaagca
     2821 gccgtagtca gtcaggttgg tcacgcgacc agtcagtttg gtaccttccg gataacgttt
     2881 agcgatagct acccacggat cttcgcccag ctgtttcagg cccagggata cacgggtacg
     2941 ttcgcggtcg aacttcagca ctttaacagt gatttcgtcg cccacgttga cgatttcgct
     3001 cggatgctta acgcgtttcc aggccatgtc agtgatgtgc agcaggccgt caacgccgcc
     3061 cagatcaacg aatgcaccgt agtcagtgag gttcttaacg atacctttaa cttccatgcc
     3121 ttcctgcagg ttttccagca gctgatcgcg ctctgcgctg ttttcggatt cgataacggc
     3181 acgacgagaa acaacaacgt tgttgcgctt ctgatccagc ttgattactt taaattcaag
     3241 ctctttgcct tccaggtgca gagtgtcacg caccggacga acgtctacca gagaacctgg
     3301 caggaacgca cgaataccgt tcagctcaac agtgaagccg cccttaactt tgccgttgat
     3361 aacaccggta acagtttcag catcttcgta agctttttcc agcgtgatcc aggcttcgtg
     3421 acgtttagct ttctcacggg acagcagagt ttcaccgaag ccgtcttcta ctgcgtccag
     3481 agcaacgtca acttcgtcac ctacctggat ttccagctcg ccctgggcgt ttttgaactg
     3541 ctcagccggg atggcggact cagatttcag accagcgtca accagtacta cgtctttgtc
     3601 gatagcaaca acaacgccac gaacgataga acccgggcgg gtttcgattt cttttaagga
     3661 ctcttcaaag agttgagcaa aagattcagt catgtttaat cttcaggttt atatttaacg
     3721 tccacctggc tccatgccgg atggggttgt ttaacatacc cgctgtcatt ccattgcaac
     3781 gggggtactg caaattcggt cgcttatgcg agagccaatt tctggcgcgc gtattgtagc
     3841 gctttttcaa tcacttgctc aatgcttaag gtggtggaat ccaacactaa agcatcggct
     3901 gccggaacca gtggcgctac cgctcggtta cgatcgcggt cgtcgcgttc tttgatctcg
     3961 gccaaaaggc gctcaaagtt aacactaaag cccttctcct gcaactgtag catgcggcga
     4021 tgcgcacgtt cttccgagga ggcgtcaagg aaaattttca ctggcgcatc agggaatacc
     4081 accgttccca tgtcgcggcc atcggcaatc aggcctggta attcgcgaaa cgcgcgttgg
     4141 cgacgcaata atgcttcacg aacgcgtggg aatgccgcga cttgtgaagc tgcattcgcc
     4201 acttcctgag tacgaatttc gccgctgaca tcttcccctt cgaggatcac ttccagattg
     4261 ccattggttg acacaaaacg tacatccaga tgggatgcca gcggtaccag cgcatcttct
     4321 gacgcaacat caacatggtg atgtaatgcc gccaacgcca gtacgcgata aattgcaccc
     4381 gaatccagca gatgccattg caatgcttcc gccatagcct tacacaaggt gcctttccct
     4441 gcaccgcttg ggccatcaat ggtaataacc ggggcaattg ccgtcatctt tatctcctta
     4501 aaacaggcgt accgttaaca taaccgcgcg cattatacgc gccgacgcca caaatcgtta
     4561 acgttgtgtg caaaaaccag ttttgtctgt attcagatgg ggatttattg cgcaattata
     4621 gcgggctggc agaagaccag cccgaaagat gacaatgatt tacttaccag aggcgatacg
     4681 atcacggatg tgttgcgcac gctctgttga tggtgggtga gagtcaaaca tggactgggt
     4741 gcgaccgcca tccagactag ccagtttttc aaagctgcca accagcccct gggtgctgat
     4801 gccacgtttc ttcaacagat caaaggagaa atcatctgct tcggactctt tatcacggga
     4861 gtatttagca ttaatagcgc cttctgcgat atcacccaat tgtgagcggg aaagctggga
     4921 agccacaccg ctggtagctg aaatggcatc gcgtgcggca acgatcgcat aagaagcttt
     4981 catttcagcc agcgagtgac ccaacgcgac gtggcccagt tcatggccca gaacaccttc
     5041 aatttcgttg tcattcatca tgtccatcag gccactgtag acacggacac agccgttcgc
     5101 catcgcccat gcgttgacgt cgctggtcat ataaacctta tagttgaccg gcgtgccatt
     5161 aatattgtta cccaatgctt tggcgatttt tgccagacgt ttaccgtact tgctggattt
     5221 acttgcgact tgattgccgc tgtccatttc ggcacagccc tgattggcaa tcgcttttgc
     5281 atcggcatcg cttaatgttg ctgctttata ggcatttaaa ccggagctga tagccatatt
     5341 ggtatcaata ccgtgggtat tttgacaccc tgtcagtaat gctgcagagg tcgcaatcgc
     5401 cagcagtaat ttagtattct tcatgtgaat catccgttga ttaatcatat ataaatatta
     5461 tgtatcaaca acttacattg acaagcgacg ctaccattaa tcaaaatgac tcacaaggtc
     5521 cgaaaaaaaa cgccgacaat g
//
LOCUS       DAZPDU010000237         5540 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZPDU010000237 DAZPDU010000000
VERSION     DAZPDU010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5540
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.240"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            80..682
                     /locus_tag="ACHURS_004122"
     CDS             80..682
                     /locus_tag="ACHURS_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFR1239720.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(684..1205)
                     /gene="ruvC"
                     /locus_tag="ACHURS_004123"
     CDS             complement(684..1205)
                     /gene="ruvC"
                     /locus_tag="ACHURS_004123"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFR1239721.1"
                     /translation="MAIILGIDPGSRVIGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(1240..1980)
                     /locus_tag="ACHURS_004124"
     CDS             complement(1240..1980)
                     /locus_tag="ACHURS_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR1239722.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(2009..2461)
                     /gene="nudB"
                     /locus_tag="ACHURS_004125"
     CDS             complement(2009..2461)
                     /gene="nudB"
                     /locus_tag="ACHURS_004125"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFR1239723.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQVVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(2579..4351)
                     /gene="aspS"
                     /locus_tag="ACHURS_004126"
     CDS             complement(2579..4351)
                     /gene="aspS"
                     /locus_tag="ACHURS_004126"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFR1239724.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTVLAELSIQVVKKAENN"
     gene            4661..5227
                     /locus_tag="ACHURS_004127"
     CDS             4661..5227
                     /locus_tag="ACHURS_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFR1239725.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            5224..5319
                     /locus_tag="ACHURS_004128"
                     /pseudo
     CDS             5224..5319
                     /locus_tag="ACHURS_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1312 a         1497 c         1328 g         1403 t
ORIGIN      
        1 tatacattgc gcctcctgaa aaaaaataaa ttttttatgc tattacgtat attcgtatct
       61 atttcaatgg aatgacaacg tgaatattaa ttaccctgct gaatatgaaa ttggagatat
      121 cgtctttaca tgtataggtg ctgccttatt tggtcaaata tcagctgcat caaattgctg
      181 gagtaatcac gtcgggatca ttatcggtca taacggtgaa gacttcctgg ttgcagaaag
      241 ccgggttccc ctttcaacca tcaccacgct atctcgtttt attaaacgtt cgtctaatca
      301 acgctatgct ataaagcgat tagacgctgg gctgacagaa caacaaaaac aacgaattgt
      361 tgaacaggtt ccctcccggc tacgcaaact ttaccacacc ggttttaaat acgaatcttc
      421 gcgccagttc tgttcaaaat ttgtttttga tatttataaa gaagcgctat gcattccggt
      481 gggtgaaata gaaacgtttg gagaactgtt aaatagcaat ccgaatgcaa aactcacttt
      541 ctggaaattc tggttcttag gttctattcc gtgggagcgt aaaaccgtca cgccagccag
      601 tttgtggcat catccgggtc tggtgttgat tcacgcggag ggagttgaaa cgcctcagcc
      661 tgaactgacc gaggcggtat aacttaacgc agtcgccctc tcgccaggtt cagccgcgat
      721 tcgctcatct gcattgcatt ctggctgacg tgacaatggg taatcgcgat agctaacgca
      781 tcggcggcat ccgcctgtgg attagcgggc agtttcagca aggtgcggac catatgctgc
      841 acctggcttt tttcggcact accaatacct accaccgttt gctttacctg acgtgccgcg
      901 tattcaaata ccggcaattc ctgattcacc gccgccacaa tcgccacgcc gcgcgcctgc
      961 cccagtttca gagccgagtc ggcatttttg gccataaaga cttgttcaat ggcgaaataa
     1021 tctggctgga actgggtgat gatttccgtc acgcccgcat agatgagctt cagacgagac
     1081 ggtaaatcat ccactttggt gcgtatgcat ccgctaccca ggtaggacag ttgcctgcct
     1141 acctggcgga tgacaccata gccgatcacg cgcgaacccg ggtcaatgcc gagaataata
     1201 gccatcacgc gtctccgttt tgctgtttag caggccccat cagagagttg ctgcaacctc
     1261 atcagagatt tcaccgttat ggtagacttc ctgcacgtcg tcgcaatctt ccagcatatc
     1321 gatcagacgc atcagtttcg gtgcggtttc tgcatccata tcagctttgg tggacgggat
     1381 catggaaact tccgcgctgt ctgctttcag acctgccgct tccagagcgt cgcgcacttt
     1441 acccatttct tcccatgcag tgtagacgtc aatcgcgccg tcatcatagg tcacaacgtc
     1501 ttcagcacct gcttccagtg ctgcttccat aatggtgtct tcatcgcctt tctcgaagga
     1561 gatcacgcct tttttgctga acagataggc aacggaacca tcagtaccga ggttaccgcc
     1621 acatttgcta aatgcatgac gcacttcagc aacggtacgg ttgcggttgt cagacagaca
     1681 ttcaatcatg attgccgtgc cgccaggacc gtaaccttcg tagatgatgg tttccatgtt
     1741 tgcatcatca tcaccgccca caccacgtgc aattgcgcgg ttcagtgtgt cacgggtcat
     1801 gttgttagac agtgctttat ccaccgccgc acgcagacgc gggttagcgt ccggatcgcc
     1861 accgcccagc ttagccgcgg ttaccagctc acgaatgatt ttagtgaaga ttttaccgcg
     1921 cttagcatcc tgcgcagctt tacgatgtct ggtgttggcc catttactat gacctgccat
     1981 aaaaatatct ccagatagcc ctgcctgttc aggcagcgtt aattacaaac tgttcaatcg
     2041 cctgccggtt gctccaggac ttagtgagcg ccgcggcagc aggcgcatca agccacttgt
     2101 aagccagatg ttcagtgaag actacctggc gttcgtgcgg gagcgcaaga cagaaccatg
     2161 attccgtatt acgcgtcacg cccggcgcat agcgatgacg taaatgtgaa aaaatttcaa
     2221 actctaccgt gcgctgacag tcaattaagg tcagttgttc agcgacaaca tcaatggtga
     2281 cctcttcctt tacttcgcgc atggcagctt gcggcgcggt ttcaccctct tccacgctgc
     2341 cggttaccga ctgccagaaa tcgggatcgt cacgccgctg caacatcagc acccgtttcg
     2401 tatcttgtgc atagatgacc actaagatcg aaacgggacg cttatacact ttatccttca
     2461 cgctgcctct tcgttgactg ccttcgctca tcccattcac atagttatct atgctcatgg
     2521 gagttcactc agttgccgcc ttgatgcaac gcgaatgatt tcgtgtattt gagtcatatc
     2581 agttattctc agccttcttc acaacctgaa tgctcagctc agccagtaca gtcgggttag
     2641 caaagctcgg tgcttcagtc atcagacacg ctgccgccgt ggttttcggg aaggcgataa
     2701 cgtcacggat attgtcggtg ccagtcagca gcatggtcag acggtcaaga ccgaatgcca
     2761 gacctgcgtg cggcggagta ccgtatttca gggcgtcgag caggaagccg aatttctcgc
     2821 gctgttcctc ttcgttgata cccagaatac caaacaccgt ctgctgcata tcaccattat
     2881 ggatacgtac tgaaccaccg cccacttcgt aaccattgat gaccatatcg taagcgttcg
     2941 ccaccgcatt ttccggtgca gctttcagtt ctgcagcagt catgtctttc ggtgaggtga
     3001 acggatggtg cattgccgtc aggccgcctt caccgtcgtc ttcaaacatc gggaagtcga
     3061 taacccacag cggtgcccat ttgctttcgt cggtcagacc aaggtcttta cccactttca
     3121 ggcgcagtgc gcccatcgcg tcggcaacaa ttttcttgtt gtcggcaccg aagaaaatca
     3181 tatcgccatc ttgcgcggca gtacgatcca ggatggcttc gatgatttct gcattaagga
     3241 acttcgctac cgggctgttg ataccttcca gacctttcgc gcgttcgtta actttgatgt
     3301 aagccagacc tttcgcgccg tagattttaa cgaagttacc gtattcgtcg atctgcttac
     3361 gggtcagcga tgcgccaccc ggaacacgta gagcggcaac acggcctttc ggatcgttcg
     3421 ccggccccgc aaatactgca aactcaacag atttcagcag atcggcaacg tcggtcagtt
     3481 ccatcgggtt acgcagatcc ggtttatcag aaccataacg gcgttctgct tccgcaaagg
     3541 tcattaccgg gaaatcgccc agatccacgc ccttcacttc cagccacaaa tgacgcacca
     3601 gcgcttccat cacttcacgc acttgcggcg cggtcatgaa agaagtttcc acatcgatct
     3661 gagtaaattc aggctgacga tcagcgcgca ggtcttcgtc acggaagcat ttaacgatct
     3721 gatagtaacg gtcaaaaccg gacatcatca gcagctgttt gaacaactgt ggggattgcg
     3781 gcagtgcgta gaatttacct ttgtgcacac gagaaggcac caagtagtca cgcgcgcctt
     3841 ccggcgtggc tttggtcagc atcggagttt cgatgtcgag gaagccgtgg tcatccataa
     3901 aacggcgcac caggctggtg attttagcgc gggttttcag gcgctgcgcc atttccgggc
     3961 gacgcaggtc gaggtagcgg tatttcagac gcgcttcttc ggtgttgacg tggttagagt
     4021 caagcggcag aacatctgcg cggttgatga tagtcagcga ggacgccagc acttcaattt
     4081 cgccggtcgc catatcgcgg ttaatatttt tttcgtcacg cgcacgtacg gtgcccgtaa
     4141 cctgaatgca gaactcatta cgcagttcag aggccagctt taacgcgtcc gcacgatccg
     4201 gatcgaaaaa tacctgcacg ataccttcgc ggtcgcgcat atcgatgaag atcaggctac
     4261 caagatcacg acgacggttg acccaaccac acagagtcac ctgctgcccc acgtgggaca
     4321 aacggagctg tccacaatat tctgtacgca tgagatatcc cttaacttag ctgccggcgg
     4381 atgccccctg ctgcgcaggt gaccaagtcg cagcgttagc tgtatgtcac aactgaatga
     4441 aaaaaggcgg ctattatact ggaaattctg ccgcaccgta agagcctggc ccgcgctgta
     4501 acgcctcgtt accactttat atcgggcctg aaatcagact ctacgccagt ttgctataaa
     4561 ggtgttgccc gaactcataa aaattaacaa aatttgtcgt tccgccatcg gctaatcgca
     4621 ttaaggtgtg aggcacgatt ttgttttttc aggagtcagc atgcttgaac ttaatgctaa
     4681 aaccactgcg ttggtggtga tcgatttaca agaaggcatc ttaccttttg ccggtggtcc
     4741 acatactgcc gatgaggtag ttaatcgcgc cgggaagctg gcggcgaaat ttcgcgccag
     4801 cggtcagccc gtgtttctgg tgcgagttgg ctggtctgcc gattacgccg aagcattaaa
     4861 acagccggtt gatgccccct cccccgctaa agtgttgccc gaaaactggt ggcaacatcc
     4921 agctgcatta ggtgcaactg acagtgatat cgaaatcatc aaacgtcagt ggggtgcgtt
     4981 ttacggtacg gatctggagt tgcaactgcg ccgccgaggt atcgatacaa tagtgttatg
     5041 tgggatctcg accaatatcg gtgttgaatc caccgcccgc aatgcctggg aacttggctt
     5101 taatctggtg attgccgaag acgcctgtag tgccgccagc gccgagcaac acaataacag
     5161 cattaatcat atctacccgc gcatcgcccg tgtgcgtagc gtggaagaga tccttcacgc
     5221 gttatgattt acatcggtct accgcaatgg tcgcatccta aatgggtgcg gttggggatc
     5281 accagccttg aagagtatgc ccgccacttt aactgcgtga tacgggattt ttaaaaatca
     5341 ctaaagaacg cccaagagca tgtgttttct ttagaataat caaagcgttg gattgccatg
     5401 ccatatgaaa atcggcaaag ttcatatgta acaaagtgtc ccaatcatgc cccaaaatgc
     5461 cccacaacaa atatttttgc cccatgcatg ccccaaaaaa tcactttccc tcactcttga
     5521 cactgtttat ccatacagtt
//
LOCUS       DAZPDU010000238         5527 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZPDU010000238 DAZPDU010000000
VERSION     DAZPDU010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5527
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.241"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            241..582
                     /gene="raiA"
                     /locus_tag="ACHURS_004129"
     CDS             241..582
                     /gene="raiA"
                     /locus_tag="ACHURS_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFR1239726.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    685..810
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            686..733
                     /gene="pheL"
                     /locus_tag="ACHURS_004130"
     CDS             686..733
                     /gene="pheL"
                     /locus_tag="ACHURS_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFR1239727.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            833..1993
                     /gene="pheA"
                     /locus_tag="ACHURS_004131"
     CDS             833..1993
                     /gene="pheA"
                     /locus_tag="ACHURS_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFR1239728.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINMSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2036..3157)
                     /gene="tyrA"
                     /locus_tag="ACHURS_004132"
     CDS             complement(2036..3157)
                     /gene="tyrA"
                     /locus_tag="ACHURS_004132"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFR1239729.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3168..4238)
                     /gene="aroF"
                     /locus_tag="ACHURS_004133"
     CDS             complement(3168..4238)
                     /gene="aroF"
                     /locus_tag="ACHURS_004133"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFR1239730.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4447..4812
                     /locus_tag="ACHURS_004134"
     CDS             4447..4812
                     /locus_tag="ACHURS_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFR1239731.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVESASQ
                     LHEVWQKGADENASTIRLN"
     gene            4962..5480
                     /locus_tag="ACHURS_004135"
     CDS             4962..5480
                     /locus_tag="ACHURS_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFR1239732.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
BASE COUNT         1519 a         1391 c         1241 g         1376 t
ORIGIN      
        1 tgagctgccg tttttttatt ctgttagttg tgaaactgaa gcgatttagt cactatcgat
       61 ctcatcaaat atggctcgct ttgagatatt cctcaagtaa aaaaacacct cttcctgcga
      121 tttctcacaa aaaagattcg ttgacaaaaa gtgacaaaat tatgagattc ccatcacaca
      181 ttttgacatc aggaacggta tgctgaattc accaagacgg gaagacaaga ggtaaaattt
      241 atgacaatga acattaccag caaacaaatg gaaattactc cggccatccg ccaacatgtc
      301 gcagaccgtc tcgccaaact ggaaaaatgg caaacacatc tgattaatcc acatatcatt
      361 ctgtccaaag agccacaagg gtttgttgct gacgccacaa tcaatacacc taacggcgtt
      421 ctggttgcca gtggtaaaca tgaagatatg tacactgcaa ttaacgaatt gatcaacaag
      481 ctggaacggc agctcaataa actgcagcac aaaggcgaag cacgtcgtgc cgcaacatcg
      541 gtgaaagacg ccaacttcgt cgaagaagtt gaagaagagt agtcctttat attgagtgga
      601 tcgccaacgc gccttcgggc gcgttttttg ttgacagcgt gaaaacagta cgggtactgt
      661 actaaagtca cttaaggaaa caaacatgaa acacacaccg tttttcttcg cattcttttt
      721 taccttcccc tgaatgggag gcgtttcgtc gtgtgaaaca gaatgcgaag acgaacaata
      781 aggcctccca aaccgggggg ccttttttat tgataacaaa aaaggcaaca ctatgacatc
      841 ggaaaatcca ttactggcgc tgcgagagaa aatcagcgcg ctggatgaaa aattattagc
      901 attactggca gagcggcgcg aactggccgt cgaggtggga aaagccaaac tgctctcgca
      961 tcgcccggta cgagatattg atcgtgaacg cgatttactg gaaagattaa ttacgctcgg
     1021 taaagcgcac catctggacg cccattacat tactcgcctg ttccagctca tcattgaaga
     1081 ttccgtatta actcagcagg ctttgctcca gcaacatctc aataaaatta atccgcactc
     1141 agcacgcatc gcttttctcg gccccaaagg ttcttattcc catcttgcgg cgcgccagta
     1201 tgctgcccgt cactttgagc aattcattga aagtggctgc gccaaatttg ccgatatttt
     1261 taatcaggtg gaaaccggcc aggccgacta tgccgtcgta ccgattgaaa ataccagctc
     1321 cggtgccata aacgacgttt acgatctgct gcaacatacc agcttgtcga ttgttggcga
     1381 gatgacgtta actatcgacc attgtttgtt ggtctccggc acgactgatt tatccaccat
     1441 taatacggtc tacagccatc cgcagccatt ccagcaatgc agcaaattcc ttaatcgtta
     1501 tcctcactgg aagattgaat ataccgaaag tacgtctgcg gcaatggaaa aggttgcaca
     1561 ggcaaaatca ccgcatgttg ctgcgttggg aagcgaagct ggcggcactt tgtacggttt
     1621 gcaggtactg gagcgtattg aagcgaatca gcaacaaaac ttcacccgat ttgtggtgtt
     1681 ggcacgtaaa gccattaaca tgtctgacca ggttccggcg aaaacgacgt tgttaatggc
     1741 gaccgggcaa caagccggtg cgctggttga agcgttgctg gtgctgcgca accacaatct
     1801 gattatgacc cgtctggaat cacgcccgat tcacggtaat ccatgggaag agatgtttta
     1861 tctggatatt caggccaatc ttgaatcagc ggaaatgcaa aaagcattga aagagttagg
     1921 ggaaattacc cgttcaatga aggtattggg ctgttaccca agtgagaacg tagtgcctgt
     1981 tgatccaacc tgatgaaaag gtgccggatg atgtgaatca tccggcactg gattattact
     2041 ggcggttgtc attcgcctga cgcaataaca cgcggctttc actctgaaaa cgctgtgcgt
     2101 aatcgccgaa ccagtgctcc accttgcgga aactgtcaat aaacgcctgc ttatcgccct
     2161 gctccagcaa ctcaatcgcc tcgccgaaac gcttatagta acgtttgatt aacgccagat
     2221 tacgctctga cgacataata atgtcggcat aaagctgcgg atcctgagca aacagtcgcc
     2281 cgaccatcgc cagctcaagg cggtaaatcg gcgaagagag cgccagaagt tgctcaagct
     2341 gaacattttc ttctgccaga tgcagcccat aagcaaaagt agcaaagtgg cgcagagcct
     2401 gaataaacgc catattctga tcgtgctcga cagcgctaat acgatgcagc cgagcgcccc
     2461 agacctgaat ttgctccaga aaccattggt atgcttccgg cttacgtcca tcacaccaga
     2521 ccacaacttg ctttgccagg ctaccgctgt ccgggccgaa catcgggtgt aaccccagta
     2581 ccgggccatc gtgcgccgcc agcatggcct gtaatggtcc atttttcact gatgccagat
     2641 caaccagaat acaatctttc ggtaaaggcg gtaatttgcc gataacttgc tcagtaacgt
     2701 ggattggcac actaacaatc accattccgg catcggcaac aatatcagcc gctcgatccc
     2761 agtcatgttg ctccagaatc cgcacctgat aacccgagag tgtcagcatc ttctcgaaca
     2821 ggcgtcccat ctgaccgcca ccgccgacga taaccaccgg acgcagtgac ggacaaagtg
     2881 ttttaaatcc tttgtcgttt tcactggagt aagattcacg catcacccga cgcaaaacat
     2941 cctcaatcag atctggcggt acacccagag cttccgcctc tgcacgacgc gaggccaaca
     3001 tagatgcctc gcgctccgga acataaatag gcagtccaaa gcggcttttc acctcgccca
     3061 cttcagcaac cagttccaga cgcttcgcta ataaattcag cagcgcttta tcgacttcat
     3121 caatttgatc gcgtaatgcg gtcaattcag caaccataat aaacctctta agccacgcga
     3181 gccgtcagct gcccgttcag atcctgatga atttcacgca gcaaggcatc ggtcatttcc
     3241 cagctaatgc aggcatcggt tacggataca ccgtatttca tttcactgcg cggttgctca
     3301 gaagactgat tgccctcgtg gatattactt tcgatcatca gaccaataat tgagcgattg
     3361 ccatctttga tttgagcaac cacggattct gccaccgcag gctgacggcg ataatcttta
     3421 ttggaattac cgtggctgca atctaccatc agagacgggc gcagtcccgc ctgttccatc
     3481 tctttttcac attgcgcaac atccgcaggg ctatagttcg gcgctttacc accgcgcagg
     3541 atgacatgcc cgtccggatt cccctgagtt tgtagcaacg caacctgccc tgcctggtta
     3601 atgccaacaa aacggtgcgg ctgggcggcg gcgcgcatag cgttaattgc tgttgccaga
     3661 ctgccgtcgg tgccgttttt aaaaccaacc ggcatggaaa gcccggaggc catttcacgg
     3721 tgagtttgcg attccgttgt acgagcacca attgctgacc agctaaacag atcgcccagg
     3781 tattgcgggc tattcggatc taacgcttcc gtcgccagtg gcagtcccat attcaccagc
     3841 tcaagcagca atttacgcgc gatctgcagc ccggcttcta catcaaaaga gccatccata
     3901 tggggatcgt taattaaccc tttccagccg acagtggtac ggggtttttc aaaatagacg
     3961 cgcattacca gatagaggct atcgctgacc tctgcggcaa gggctttaaa tcgacgagca
     4021 tattccagag cagtttccgg atcatgaatg gaacaaggac cacaaactac cagcagacga
     4081 ggatcgcgcc cggcgataat atctgaaatg gttttacgcg agtcagcaat ctgggcttct
     4141 tgttgcaggc tcaatggaaa agcggccttc agttgttccg gagtcattaa aacctgttcg
     4201 tcggtaatat gtacgttatt cagcgcgtct ttttgcatga tggcgatcct gtttatgctc
     4261 gtttgcgata gttgatcctc agcgaggatg acgtaacgat aacacataaa gtaaagtttt
     4321 caatccatat ttcgtacatt tttctttaca cgaacaattt gctcgcgctt ttgatgctta
     4381 tttcgtacaa ataaatttac acctccttta aaaacaatcc gctatgcttt gaaaaaggag
     4441 aaagaaatga tgaaaaagtt tatcgccccc ttattggctt tactggttag cggatgtcag
     4501 attgatcctt atactcacgc gccaaccttg accagcaccg actggtatga tgtcggtatg
     4561 gaagatgcga tatcgggcag cgccataaaa gatgacgatg catttagcga ttcacaggcg
     4621 gatcgcggtc tataccttaa aggatatgcc gaaggacaaa agaaaacttg ccagaccgat
     4681 tttacttatg cccggggact ttccggtaaa agctttcctg ccagctgtaa caatgttgaa
     4741 agtgccagcc aactgcatga agtatggcaa aaaggggctg atgaaaatgc cagcacaata
     4801 cgattaaatt aattaaaacc ccacaaataa attaaacata agattttact cattgtgttt
     4861 tatttctcac attgatgacg atcgcatgaa aaaatgatat tttaatgagc attctgaaaa
     4921 aatatcgatg catttcgagc gaagatggtg gggatccctg aatgcgtttt tctcaccgac
     4981 tgttcttact tctgattctc ctgctgacgg gagcaccaat actcgctcag gagccttctg
     5041 acgttgcgaa aaacgtgcgt atgatggttt ccggtattgt cagttacacc cgctggcctg
     5101 cgctatcagg gccaccaaag ttatgtatct tttcatcttc acgttttagt accgccctac
     5161 aagagaatgc cgcgacatct ttgccttatc tgcctgtcat cattcacact caacaagagg
     5221 cgatgatttc aggctgcaat ggtttttatt ttggcaacga gtcaccgaca ttccagatgg
     5281 aattaaccga acaatatccg tcaaaggcgt tattattaat tgccgaacaa aataccgagt
     5341 gcattattgg tagcgccttt tgtctgatca tccacaataa tgacgtcaga tttgccgtaa
     5401 acctggatgc cttatcgcgt agcggtgtaa aggtcaaccc ggatgtctta atgctcgcac
     5461 ggaagaaaaa tgatggataa cgataattct cttaataagc gccccacgtt taaaagagca
     5521 ttacgca
//
LOCUS       DAZPDU010000239         5466 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZPDU010000239 DAZPDU010000000
VERSION     DAZPDU010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5466
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.243"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..1334)
                     /locus_tag="ACHURS_004136"
     CDS             complement(<1..1334)
                     /locus_tag="ACHURS_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFR1239733.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     AGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNAA"
     gene            complement(1380..2138)
                     /locus_tag="ACHURS_004137"
     CDS             complement(1380..2138)
                     /locus_tag="ACHURS_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239734.1"
                     /translation="MDALQTQTVNSTTAPQPSYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSKIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            complement(2170..2883)
                     /locus_tag="ACHURS_004138"
     CDS             complement(2170..2883)
                     /locus_tag="ACHURS_004138"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR1239735.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGLIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGARILELDGR
                     QPADEILQASLRAIHAALS"
     gene            3057..3749
                     /locus_tag="ACHURS_004139"
     CDS             3057..3749
                     /locus_tag="ACHURS_004139"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR1239736.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPRPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(3798..5297)
                     /gene="pitB"
                     /locus_tag="ACHURS_004140"
     CDS             complement(3798..5297)
                     /gene="pitB"
                     /locus_tag="ACHURS_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFR1239737.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            5234..>5466
                     /locus_tag="ACHURS_004141"
     CDS             5234..>5466
                     /locus_tag="ACHURS_004141"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239738.1"
                     /translation="MPEQAITKALCIYQGQQINLAYIRLRHFEFTNGRIISDFNGMGK
                     VKYHFLTIKRVLFLVYQTISIHSKPLEIIKTQV"
BASE COUNT         1463 a         1424 c         1317 g         1262 t
ORIGIN      
        1 gctgcgttat aaagcaagga atgacgctga aaaaatgcct ttaaagttgg aattaacgag
       61 agcaccacat ccagacattg cccaccgaag taagcaaacg ctgctcccag cacaccaaac
      121 agatgacaga acatgaacag caatacgatg tagccgattg aggcgatggt ttgcgccacg
      181 agaaaagcgc gctgttttcc cgccatcaat agcaatgact cctgcgggaa acccagcatc
      241 gaaatcacaa ttgcgcccag catcacctga atcaggtcat acgcttcgag atacttaacg
      301 ccaaacacca gcgaaatgag cggcttgccg gcgatcaaca ccgcgagcgc caccagaata
      361 ccgattccgc ccgccagtaa accggacttc acacctaaca accacggtct ggtggtgcgc
      421 ggatctaaac gcatcacctc tgggtaaaaa cttttaccca gcaaacccgc tggcgttccg
      481 gcggcgtcga agaatgtcat ggcgatttta aataacccgg cggcagcggg tcctaacacg
      541 atccccacca gcactgtgct gcacgagtta cgcgccgacc agatggagtg ggcaatgttg
      601 gttgaccaga caaaactcca cgcgcctttg atgtgtcggg cagactcaaa cagattcaat
      661 ttgaaggcat tatggatatt tctgcggcgt aattcgcgcg ctgcaaacca ccagtacatg
      721 gtgccgccaa ccagattcga cacgtaccag gcaataacaa aacccgcaaa accaaagtca
      781 aaataccagg ctacgacgct ccccgctgcg cgcagaaaag gtttcgtcgc ctgctgtaca
      841 gcaattaaat cgaagcgatc taccgcacgc agaatgccgg tcggcgtgga ggaagccatt
      901 gaaggaatga gcgtgcaata cagcgccgcc agccaaaagc tttggtcatc cagacctaat
      961 gaatgggaaa ggaatggcag taaggcaatg ccgccgacaa tcgccaccgc gccgctgacg
     1021 atatccagcg agaaggaaaa tgagacgaca ttgcgaaatt gctgcggatt attgttggtc
     1081 aaggctggcg ttccgtactg aaccaccagc tgccatgtct gaaacttaat aaaatcgctg
     1141 atcgacttgg cgtacgattg cacaatcacc agtacgccaa acatggcggg cgtcatccct
     1201 ttaccggcac acgagagcgc caacagcccc agcaaggcgc tcacgacatt actggagcct
     1261 aaccaggcgc tattgcgaat aatggtgcga aacgcgccat ctgcaaacca atgtttgatg
     1321 ttaaaacccg ccaattcagc ctgaccttat cgttgatagt aaaaagtatc ccgccagcct
     1381 taagttaaac ttcggcggtc agaaacgatg gcaaccagag aaaccgcctt ctgtgcctgt
     1441 tccagcactt cgctgtaggg cgctctggaa tcaatctcaa gaatttttgt gccgttatag
     1501 ccaatcttcg acatgacacc gattttgtcc tgcagctcgg catagtcatg gtcaggcttg
     1561 cgggagatgg cagtctcaat atcaatgccc aggcgaataa ttaattccgg gcgatattgc
     1621 gccatttgtt ggtataaacg ccgttcgcgc tgcgccagaa acatgctgat tttcccggtc
     1681 gcacgttcga cgccaatccc cggtccgtcg taataaaagc ccgaaatttc agcctgcggg
     1741 aagcgatcgc tgaccaccag aacgccactt tgcgccagtc gctgaacctt gcgtagattc
     1801 gccattcttc gcagcgagaa gcagtacata atcaccgccg cccatagcgc cggagatttg
     1861 gttttcatgc tttgggtttt cgaggatttg gccgccagtc gccgttccag ccagacgcca
     1921 accaacggta atcgtttgat tttgtcgccg tcttcgcccg agagcagccc cagatagcgc
     1981 cgctcggttt gccagtgctg ttgcaatgat ttcaccagat cggtggtcag cgtggattta
     2041 ccggtgccat cgcacccgac caccgcaatt agccccggaa tgtagctggg ctgcggcgcg
     2101 gttgtactat taacagtttg tgtttgtagt gcatccattc gttaaatcat cccctatcca
     2161 ttttggtgat caggaaagcg cggcgtgaat tgcacgtagt gacgcttgca ggatttcatc
     2221 ggctggttgc cgaccatcca gttcaaggat cctggcgccg ttgaaagtca gttgcggcgt
     2281 aacggcgatt ttttcctgta acgctgccag ttggtggtca ggtttacgcg caaacgcggt
     2341 ttgttcatca atgccaagac gaatcaacaa tacgggcaaa taagatgcca tccattggta
     2401 cagcttcagc tcgcgctgcc ttaacatttt tatccaaccg ttaccgcccg tggtttttgc
     2461 caattgcggg ccatcaaagc gaaaacccgg cacttcaacc tgcgggtagc ggtcggtgat
     2521 gagcagaaag ccttgctggc ttttacacaa cattttgcga aacttgtacg cccgccagca
     2581 ggaaagcaga tagataacca gagcagtaat attgccaggc ggtgttgagg gtttttcgtg
     2641 cacatgtgcc gctttacttc gcagataacg cccaaaaggt gcgccaataa cagggagctg
     2701 tgaaatccat tcgccaatta gcccggacga ttgcccgaga taaatgtgtt ctgttggcat
     2761 tcttgctgcc agttcattta ccaggcttgc cgtgagggtc gatttacctg aaccatcaca
     2821 tcctacaatg gcaataacac gcactggcgt ggaattaatt attgacatat tacgttgatt
     2881 cacgaaaaac ccggcagtaa ataatgtatt gaatattacg tggtccgtgc tcagaatatc
     2941 cgttcaacct tattgtgaca atggttaatt gtgacattga cctgaattac gctttcataa
     3001 aaacatatta accaaataaa tatttttaat ggatatttaa attaaaggat atattcatgc
     3061 agtcaataac acctccatta attgccgtta ttggtagcga tggttcaggc aagtcaacgg
     3121 tgtgtgaaca tcttattacc gttgtcgaaa aatatggtgc tgccgaaaga gttcatttag
     3181 gaaaacaggc cggaaatgtc ggtcgtgcag tgacaaaatt accgttgatg ggaaaatcct
     3241 tacataaaac aattgaacga aatcaggtga aaacagcaaa aaaattgcct agaccagttc
     3301 cggcgctggt aattacagcg tttgtcgccc gtcgcttact gcgctttcgt catatgcttg
     3361 cctgtcgtcg tcgcgggtta attgttctaa ccgaccgtta tcctcaggac caaattcctg
     3421 gcgcttacga tggtacggtg ttcccaccta acgttgaagg tggtcgtttt gtctcatggc
     3481 tggcaagcca ggaacgtaaa gcgtttcact ggatggcgag ccataagcct gatctggtca
     3541 tcaaactcaa tgttgacctt gaagttgcct gtgcacgtaa acccgaccat aaacgggaat
     3601 cgctggcgag gaagattgcc ataacgccac agttaacctt tggtggtgca caactggttg
     3661 atatcgatgc caatcagcca ctggaacagg tgttggttga tgcagaaaaa gcgattacgg
     3721 attttatgac cgcgcgtggt tatcactagt caaaaatgga aatgcccgat cgccaggacc
     3781 gggcattttc aggaaggtta aatcaactgc aatgctatcc agtacagccc accagaaaga
     3841 aaaattgccg ccggtaaagt aaacacccac gccatcagga tgctggttac cgttttacgc
     3901 tgtaacccac cgccgtccac caccatcgtc cctgctaccg cagacgagag gacgtgtgtt
     3961 gtggagacag gcatcccaat ataactggca aggccgatag acactgccgc cgtcatttgt
     4021 gccgccatgc cttgcgcata cgtcatgccg cgcttaccaa tcttctcacc gatggtcatc
     4081 gctacacgac gccagccaat catggtgcca atgccgagcg ccagtgctac cgccatgatg
     4141 atccacacag gagcgtactc aatggtgctt aacatatcgc tgcgcagttt tttcagcagg
     4201 ttctggtctt ctttactgac gcctggcagt ttcgccagct tcgcggaggt atcagagatg
     4261 cacagcataa tgcggcgcag ctggctgcgc tgactcacgc ttaacggctc gtaactttcc
     4321 atattgcctg gcagcatcgt tttaacgcgc gcaatcgcat caaaggtatt tgccggatga
     4381 cagtgaaact ctgttacttg cgtgccatca gtcgatgctg caggcaatgg aggttccatc
     4441 gcaatcaact tctgcggcag ttcaggatgc tgttgcaggt agtgttcgaa gttggtgacg
     4501 gcatcgcggg tacgggtaat ttcatagccg gacgcgttca tattgacgac gaagccagca
     4561 ggggcaatcc ccaccagtac cagcatcacc aggccgatcc ctttttgtcc gtcgttcgcg
     4621 ccgtgcgaaa acgccacgcc cgcagcggaa acaatcagcg caatacgcgt ccagaatggc
     4681 ggtttacgtt tgcctttttt ctttttgcga tcttccggaa tgcggtgaat acggtcacgc
     4741 tttttcgtcc cgctccagta gcgtcgcagc aggaatatca ggcctcccgc aatgaccagg
     4801 ccgacgatag gggaaacaat cagcgaggag aaaattttgg tcacttcacg caggtttaac
     4861 gcatccatca ccgatgatcc ggttaacagc gcgttggtta aaccgatgcc gataatcgca
     4921 ccaatcaagg tgtgcgaact ggaggccggt aaaccgaaga accacgttcc caggttccag
     4981 ataatcgccg ccagcagcat ggaaaagacc atcgccaggc cgtgggttga ccccatattc
     5041 agcaacaaat cggttggcaa catatggaca atggcatagg caacgctaag tccgcccaat
     5101 aacacgccaa aaaagttaaa aaatgccgcc atcaccacag caagttgtgg ttgcatggca
     5161 cgagtataaa taacggttgc caccgcattc gccgtgtcat gaaaaccatt gattgcttcg
     5221 tagaacaaca caaatgccag agcaagcaat aacaaaagcc ctgtgtatat atcaaggcca
     5281 acaaataaat ttagcatata ttagattacg ccattttgaa tttacgaacg gacgcattat
     5341 cagtgacttt aacggcatgg gcaaagtgaa atatcatttt ttaacgataa aaagagtgtt
     5401 atttcttgtg tatcagacaa taagcattca ttcaaaacct ttagaaataa tcaaaacaca
     5461 ggtatt
//
LOCUS       DAZPDU010000240         5409 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZPDU010000240 DAZPDU010000000
VERSION     DAZPDU010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5409
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.244"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(64..366)
                     /locus_tag="ACHURS_004142"
     CDS             complement(64..366)
                     /locus_tag="ACHURS_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239739.1"
                     /translation="MRNRANTSPRDEAAFINGGTAGLDNTKKSSDKSSVVSKAKPVSI
                     SFFEENLKDIDALITAEMVSGKGRVNRSDVVRAAVEALKTLSKTEVSRLIAEVKQR"
     gene            complement(363..989)
                     /locus_tag="ACHURS_004143"
     CDS             complement(363..989)
                     /locus_tag="ACHURS_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family plasmid-partitioning AAA ATPase"
                     /protein_id="HFR1239740.1"
                     /translation="MIIVLGSQKGGVGKSTLAVSIAAYLMSLGNRVIIVDADDQKSVL
                     TWYNNRPEDLPHIPVTGATGNIKAMLKEHEKSYDFVIADCAGRDSAEMRSGLMAADVF
                     ISPLRPSQMDLDVVPHTCSVFTAAKDFNEDVRGYLVLNMTPTNMFVNEANEAAEVLKD
                     YPEMHLANSRVCDRKAHRDAWAESMTIFETQNDKAQQEIEALVKEVIL"
     gene            complement(1185..2456)
                     /locus_tag="ACHURS_004144"
     CDS             complement(1185..2456)
                     /locus_tag="ACHURS_004144"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0019985 - translesion synthesis [Evidence
                     IEA];
                     GO_process: GO:0042276 - error-prone translesion synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFR1239741.1"
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPEA
                     KALGIRMGQPWFQVRQMRLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAVEPYSIDE
                     MFIDLRGINHCISPEIFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFS
                     GVVALTAENRNRILKLLGLQPVGEVWGVGRRLTEKLNALGINTALQLAQANTAFIRKN
                     FSVILERTVRELNGESCISLEEAPPAKQQIVCSRSFGERITDKDAMHQAVVQYAERAA
                     EKLRGERQYCRQVTTFVRTSPFAVKEPCYSNAAVEKLPLPTQDSRDIIAAACRALNHV
                     WREGYRYMKAGVMLADFTPSGIAQPGLFDEIQPRKNSEKLMKTLDELNQSGKGKVWFA
                     GRGTAPEWQMKREMLSPAYTTRWTDLPVAQL"
     gene            complement(2456..2893)
                     /gene="umuD"
                     /locus_tag="ACHURS_004145"
     CDS             complement(2456..2893)
                     /gene="umuD"
                     /locus_tag="ACHURS_004145"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFR1239742.1"
                     /translation="MSTVYHRPADPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDL
                     NSYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKR
                     LLLRPRPALEPVSDSPEFRTLYPENICIFGVVTHVIHRTRELR"
     gene            complement(2890..3138)
                     /locus_tag="ACHURS_004146"
     CDS             complement(2890..3138)
                     /locus_tag="ACHURS_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR1239743.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRID
                     QGSAPSVSVTGARNDKDKERILSLLEEIWQDDSWLPAA"
     gene            3557..4459
                     /locus_tag="ACHURS_004147"
     CDS             3557..4459
                     /locus_tag="ACHURS_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFR1239744.1"
                     /translation="MYFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSPENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEELPDDVRETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDIEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVVAELSRRFGCTLEHWYAEQGCNFCGWQLYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIIDNVAHYGG"
     gene            complement(4410..4640)
                     /locus_tag="ACHURS_004148"
     CDS             complement(4410..4640)
                     /locus_tag="ACHURS_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HFR1239745.1"
                     /translation="MSPTPAGSGKSARPVPGRTRDTDHRQRLCRAASAGAVKRGARRH
                     PLSEKKRAVARPSPSYFIRHNAPHCRLSTQVR"
     gene            4556..4768
                     /locus_tag="ACHURS_004149"
     CDS             4556..4768
                     /locus_tag="ACHURS_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015589601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239746.1"
                     /translation="MNAACGQCPASGRARDGRFYRSRLASVTVYASPSPFSDERPSSR
                     FRGIALPSKRRRLRYSTVGLTRYRTR"
     gene            4844..>5409
                     /locus_tag="ACHURS_004150"
     CDS             4844..>5409
                     /locus_tag="ACHURS_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFR1239747.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRY"
BASE COUNT         1230 a         1401 c         1362 g         1416 t
ORIGIN      
        1 taacaagata acaagataac aagataacaa gataacaagg tgttatcttg ttatctcaag
       61 gtgttatctt tgttttactt ctgctatcaa tcgtgatacc tctgtttttg atagtgtttt
      121 aagtgcttct accgccgccc tgacaacatc agaacgattc actctgcctt tacctgaaac
      181 catctcagcc gttataaggg catcaatgtc ttttaggttt tcttcaaaaa aagaaatact
      241 cactggtttt gctttgctta caaccgatga tttatctgat gactttttgg tattatcaag
      301 gccagccgtg ccaccgttaa taaatgctgc ctcgtcacga ggtgaagtgt ttgctctatt
      361 tctcacagaa taacctcttt cactaacgct tcgatttctt gctgggcttt atcattttgc
      421 gtttcaaaaa ttgtcattga ttcggcccaa gcatctcgat gtgctttacg atcacatacc
      481 cgggagtttg ccagatgcat ttcagggtaa tccttaagta cttcagctgc ctcattggct
      541 tcattaacaa acatatttgt gggcgtcatg tttaaaacga gatatccccg aacatcctca
      601 ttaaaatctt ttgctgctgt aaaaacggag catgtatgag gcacaacatc caggtccatc
      661 tgagatgggc gcaaaggaga aatgaataca tctgcggcca tcaatccact acgcatttct
      721 gcgctatcac ggccagcaca gtctgcaata acaaaatcat aggatttttc gtgttccttt
      781 aacatggctt tgatgttacc tgtcgcgcct gtaaccggaa tatgagggag atcttcaggg
      841 cgattgttgt accaggttaa tactgatttc tggtcatctg cgtcgacaat aatcactcta
      901 ttgcctagtg acatcagata cgcagctata gataccgcca gtgtactttt acctacccca
      961 cccttttgac tacctaatac tattatcatt atagcctcat attaagctgt taagttaaca
     1021 acttaatatt gccatctgaa cgcgaaccat gcaattattt ttgttatgtt aacaacttag
     1081 caagttgtta agtatttgat ttatagatta attaagataa caagataaca agataacaag
     1141 ataacaagat aacaggataa caatattatg ggtggattac atattcataa ctgggctact
     1201 ggcaaatctg tccatctggt ggtatacgca ggagaaagca tttcccgttt catttgccat
     1261 tcaggggcgg ttcctcgccc cgcaaaccac actttccctt ttcccgactg gttcagttca
     1321 tcgagtgttt tcattaactt ttcactgttt ttacggggct ggatttcatc aaataatccc
     1381 ggctgcgcta tacccgatgg tgtgaaatca gccagcatga cacctgcctt catatagcgg
     1441 tacccttcac gccagacatg gtttaacgct ctgcatgctg cggcaataat gtcccggctg
     1501 tcctgtgtgg gcaatggaag cttttccaca gcggcattgc tgtaacaggg ttcttttact
     1561 gcaaaggggg atgtccgtac aaatgtcgtc acctgccggc aatactgacg ctccccacgt
     1621 agtttctctg cggcccgctc tgcatactga acaacagcct ggtgcatggc atctttgtct
     1681 gtgattcgtt caccaaaact gcgactacag acaatctgct gttttgccgg cggtgcttct
     1741 tccagagata tgcaggactc gccgttgagt tcgcgtaccg tacgctcaag aatgacgctg
     1801 aagtttttcc ggatgaatgc cgtgttagcc tgcgccagct gcagtgctgt gttaataccc
     1861 agcgcattca gcttttccgt cagtctgcgt cctactcccc agacctcacc aactggctgc
     1921 aaccccagta gcttcaagat ccgattacgg ttttctgccg tcagcgcgac cacaccggaa
     1981 aactgtggcc attgctttgt tgcccactgg gcacttttag ccagcgtttt tgtaggcgca
     2041 atgcccaccc ccatggtgag tcctgtccag ctctttacct gttccctgag ctgatgacca
     2101 aaaatctccg gagagatgca atgatttatc ccccgcaaat caatgaacat ttcatcaatt
     2161 gagtagggct caactgcggg agaaagcgac tccagaacag ccataacccg ttggctcatg
     2221 ctgtggtaca gcgcataatt gctggaaaat acatgtattt tcttctccag gcgcatttgt
     2281 ctcacctgaa accagggctg ccccattctg atgccaaggg cttttgcctc cgggctgcgc
     2341 gcgatcacac agccatcgtt attgctgagt acgatgaccg gttcgttgcg aaggtccggg
     2401 cggaaaactt tttcacatga ggcgtagaaa ctgttgatat cagccagtgc aaacatcagc
     2461 gtaactcccg cgtcctgtgt atcacgtgag tgacaacacc aaaaatacag atgttttccg
     2521 gatacagtgt gcggaactcc gggctgtctg aaaccggctc cagtgccggg cgtgggcgca
     2581 acagcagtcg tttgacggtg aactcaccgt cgatctcagc gataacgatg tccccgtgtt
     2641 gtggtttttc ggccctgtcc actaccagca gatcaccatt ctgcacgcca gcctggttca
     2701 tcgattcacc gctggcgcgc agaaagaagg tggctgcagg tctgctgatg caatagctgt
     2761 tcagatccag ttcctgctca gcataatcag tggcaggcga aggaaaaccg gcctggcaac
     2821 gatcggcaaa caacggtcgc acgtaactgt catcgcctga agggtcagcc ggacggtgat
     2881 aaacggtact catgctgcag gcagccagct gtcgtcctgc cagatttctt ccagcagaga
     2941 caggatacgt tctttatcct tgtcgttgcg ggctcctgtg acagatacag acggggcgct
     3001 gccctggtct atacgcagcc agatttccgg gtagtgtggt ttcagacgtt gttcgatttc
     3061 attctgtagc gcctgaagtg tgcctgattt gaggtttttt gtgctctggc ggtcgaaaag
     3121 aatttcaatg cggatcatgt tctcatctcc cttttcttta tgctgtatgt ttatacagta
     3181 ttctaataac tgaatccgca cacagtcaaa tgtcattgtt gtgactaaaa aacgctcaat
     3241 tctctgagtg tgtggctgta tgcctgctgt gggctgctgt gtccagctta tccacagcat
     3301 tttgtgcacg gttctgtgga caaaatacct ggttacccag gccgtgccgg cacgttaacc
     3361 gggctgcatc cgatgcaagt gtgtcgctgt cgccggcctc ctcacccggt cacgtttcgt
     3421 cgttcctcct ccacgcgctg cggcttcggg gccgcacctg cattcgtatg cggtcgcccg
     3481 gtaacaggtg cggcacggcc tgatggaggc cgcatgtgag aggggaattc ccatgccaaa
     3541 ctggtgctcg aatcgtatgt atttttctgg tgaaccggca cagatcgctg agattaaacg
     3601 actggccagc ggtgcagtca caccgcttta tcgccgcgcc acaaatgaag gtattcagct
     3661 gtttctggcc ggaagtgccg gacttctgca gatcactgaa aatatccgct ctgagcagtg
     3721 ccccggagtg acggctgccg gacgtggtgc tgtatcaccg gaaaatatcg cgttcacccg
     3781 ctggctgaca cacctgcaga acggtgtact gctggatgag caaaactgcc tgatgctgca
     3841 tgaactctgg ctgcagagcg gtactggaca gcgtcgctgg gaagagttac cggatgatgt
     3901 cagggaaact atcactgtgc atttcaccgc aaaaagaggt gactggtgtg acatctgggg
     3961 taacgaggat gtatcggtgt ggtggaaccg tctgtgtgac aacgtactgc cggaaaaaac
     4021 catgccgttt gacctgctga cggttttgcc gacccgcctg gatattgaag tgaatggctt
     4081 taacggcggt gttctgaacg gtgttccttc tgcatatcac tggtatacag aacggtatgg
     4141 cgtgaagtgg ccttgtgggt atgacctgaa tatcagcagt cagggggaaa acttcattca
     4201 ggtagatttc gacacgccgt ggtgtcagcc ggaaagcgac gttgttgcag aattaagccg
     4261 ccgtttcggc tgcacgctgg agcactggta cgccgaacag ggctgcaatt tctgtggctg
     4321 gcagttgtat gagcgcggag agctcgttga tgtgctgtgg ggggaactgg aatggtcttc
     4381 cccgacagat gatgatgagc tgccggaagt caccggacct gcgtggataa tcgacaatgt
     4441 ggcgcattat ggcggatgaa gtatgacgga gacgggcggg caaccgcccg tttcttttcc
     4501 gacaaaggat gtcgccgtgc tcctcttttt actgcgccgg ctgacgcggc gcggcataaa
     4561 cgctgcctgt ggtcagtgtc ccgcgtccgg ccgggcacgg gacgggcgct tttaccgctc
     4621 ccggctggcg tcggtgacag tgtacgccag cccgtcgccc ttttctgatg aacgcccttc
     4681 aagccgcttt cgcggcatag ccttgccgtc aaaaagacga cggctgcggt attccacggt
     4741 cggcctgacc cgttaccgga cgcggtgaac agcccacagg cagcggggaa cgggcaccac
     4801 aggggtgccc tcccggtgcc ctctgtaaga gaaggagttt ggtatgtccc gttttgtcct
     4861 cggtaactgc atcgatgtta tggcccgtat ccctgataac gccattgatt tcatcctcac
     4921 cgacccgccg tacctcgtcg gttttcgtga ccgtcagggg cgtaccattg ccggcgataa
     4981 aaccgatgaa tggctgcaac cggcctgtaa tgagatgtac cgcgtactca aaaaagacgc
     5041 gttaatggtg agcttctatg gctggaatcg cgtcgatcgt tttatgtctg cctggaaaaa
     5101 tgcgggattc agcgttgttg gtcacctggt cttcaccaaa aactacacat cgaaggccgc
     5161 atatgtgggc tatcgccacg aatgtgccta catcctggca aaaggccgtc cacgtctgcc
     5221 acaaaacccg ctgccggacg tgctgggctg gaaatattcg ggcaatcgcc atcacccgac
     5281 ggaaaagccc gttaccagcc tgcaaccact gattgagagc ttcacacatc cgaacgcgat
     5341 tgtgctggac ccgtttgcag gcagcggatc aacctgcgtg gccgctcttc agtccggacg
     5401 ccggtatat
//
LOCUS       DAZPDU010000241         5345 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZPDU010000241 DAZPDU010000000
VERSION     DAZPDU010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5345
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.245"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(57..1340)
                     /gene="aroA"
                     /locus_tag="ACHURS_004151"
     CDS             complement(57..1340)
                     /gene="aroA"
                     /locus_tag="ACHURS_004151"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFR1239748.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1411..2499)
                     /gene="serC"
                     /locus_tag="ACHURS_004152"
     CDS             complement(1411..2499)
                     /gene="serC"
                     /locus_tag="ACHURS_004152"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFR1239749.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2698..3390)
                     /locus_tag="ACHURS_004153"
     CDS             complement(2698..3390)
                     /locus_tag="ACHURS_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFR1239750.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            3520..5280
                     /gene="ycaO"
                     /locus_tag="ACHURS_004154"
     CDS             3520..5280
                     /gene="ycaO"
                     /locus_tag="ACHURS_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFR1239751.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         1366 a         1380 c         1266 g         1333 t
ORIGIN      
        1 caatgtgccg acgttttgat aaagaaagat ttggctattt attgcccgtt gttcattcag
       61 gctgcctggc taatccgcgc caactgctcg aaataatccg gaaatgtttt ggccgtacat
      121 ttgggatcaa gaatcgtcac tggtgtatct gacaacgcca ccagcgagaa acacatcgcc
      181 atccggtgat cattgtatgt cgcgatctcg gcaaagttca gtttttccgg tggagtgata
      241 cgaatgtaat cgtgcccctc ttctacttcc gcaccgactt tacgcagttc tgttgccatc
      301 gcaaacaggc gatcggtctc tttaacacgc cagttataga tattgcgcag cgtggtggtg
      361 ccttttgcaa ataacgccgc cgtggcaatg gtcatcgccg catcgggaat atggttcata
      421 tccatatcaa tagcgttcag ttcaccacgc gtgcaggaaa tataatcatc gccccagcaa
      481 atggtcgcgc ccattttttc cagcacatca gcaaagcgaa tatcaccctg catactgtta
      541 cgtccaatac cggtcacttt tacagtgccg cctttgattg ctgctgctgc cagaaagtaa
      601 gaagccgaag atgcatcgcc ttcgaccaaa taagtacccg gagactgata agactgcccg
      661 ccttttacga caaattgttg atagtgctga ttttcaattt caacaccaaa cgtcttcatc
      721 agattgagtg tgatgtcgat ataaggttta gaaaccagat cgcctttaat acgaatcacc
      781 gtatcttccg gcgcaagagg cgcagtcatt aacagtgcgg tgaggaattg gctggaaacg
      841 gagccatcaa cgtcaacgtt gccgccagta aagccgccct gtaaacgcaa cggcggataa
      901 ttttcttgtt ccaggtaagt gatcttcgcc ccgcccaggc gcagcgcatc caccagatga
      961 ccaatcgggc gttctttcat acgcggctca ccggtcagca caatatcatt gctacccaga
     1021 caaagagctg ccgccagcgg acgcattgcc gttccggcgt taccgaggaa caactccagg
     1081 gcaccttctg cgtgtaatgg accgccgtta ccgataattt cgcaacgcgt acgatcggct
     1141 gaaagcgtat agcttacccc taacgctgtt aatgcattca gcatatggcg cacgtcatcg
     1201 ctatccagca gattggttaa tactgttttg ccgtgtgcta atgccgccag caataaagcg
     1261 cggttagaaa cgctcttgga accgggcaga ttaatagtgc catcgacacg agcgatgggt
     1321 tgtaacgtca gggattccat gaactcaact ctctacaaca gaaataaaaa ccccacaggc
     1381 tggctgtggg gattaagcaa aatttcggca ttaaccgtga cggcgttcga actcaaccat
     1441 gaagtctgtc agcgctttaa cgccttccag cggcatggcg ttataaatag aagcgcgcat
     1501 tccgccgacc acacggtgac ctttcagtgc atgaaggcca gcagcaaaag actcttcaag
     1561 gaacaatttg tcaagcgcac tgtccgccaa ctggaacggc acgttcatcc gcgaacggtt
     1621 agctttcgcc acgtcattgc ggtagaaatc gctgttatca atcaccccat atagcagttc
     1681 tgctttttgc tgattgattt tatccatttc agctacaccg ccgttcgctt tcagccattt
     1741 aaagaccaga ccagatagat accaggcaaa tgtcggcggc gtgttaaaca tggagccgtt
     1801 atcgttgagg atggaataat ccagaatcga cggacacgcg atattcgctt tgcccagcaa
     1861 atcttcacga acgatgacga ttgtcaggcc agccgggccg atatttttct gcgcgccagc
     1921 gtaaatcacg ccataacggc tgacgtcaat cggacgggaa agaatggttg aagagaagtc
     1981 ggcggcgacc accacatctt tgccgaagtc tggcgtttcg tcgatggcga taccgtcgat
     2041 ggtttcattc gggcagtaat gcatataagc agcattatca gagagttgcc attcacgcat
     2101 tggcttaacc gcgcgcagac catcaacagt cactttggcg tcaaagacat taggcgtgca
     2161 gtattttttc gcttctttaa tggcacttgc cgcccagtaa ccggcatcaa cataatctgc
     2221 ggtggtttta tcaccgagaa tattcagcgg taccgcagca aactgaccgc gaccaccgcc
     2281 atggcagaat aataccttgt agttggaggg gacattaaga agatcgcgaa aatccttctc
     2341 ggcttcctct gcaacctgaa tgaactcttt gccacggtga ctcacttcca tcaccgacgt
     2401 accaagaccg ttccagtcgc gcagttcctg ttgagcctgt ttaagcacct ctgccggtag
     2461 cattgccgga ccagaactaa aattgaagat ttgagccatt gcccctcacc acgttgcgtt
     2521 gctattaacc cgcggtcaat cgattgcgac cacaggtaat acattattcc tgtggctatc
     2581 ggttttatca ttcagtgaca cacatcgcaa tgagtaaaac cgttgagggc aaaaatgtgg
     2641 cttgcatcac acaaaagaat ctctcggatt attagcaaaa agccgatgtt tttactgtca
     2701 ggtcacccgt tttgcccgac ttgccttcgt ccattctgga tttgcacagc gcggacataa
     2761 ttgtttttcc cccgctttca tatgaatgat ttcactacaa cgaatacagg catagtccgc
     2821 cgactccggc accactttgt aacgctgcgt acaatcactg ggtaaaataa gtaaacccgg
     2881 tttcaccttg tcatcttcaa agaaatagac actgatttgc ccgttggttt cgagaatcgc
     2941 cagacgtacc tgccccagct gctccacgcc acgcaatcgt agctccataa agaactcaaa
     3001 ttccgtcatg ttggagttat tgagtttcga ccaggccagc tcgccatctt caataataac
     3061 aaccggctta ccttccagta gatcttccag tttttcactg tgcgccatca accacattac
     3121 caggcggtat aacaacgcca gagtgataaa gacgataagt accggcacca tcggcacatc
     3181 atcataaaac gccacatctc ccgccgctga acccagcgtg agaatgatta acacttcaaa
     3241 cagcgacatc tgtcgcacac cgcgtcttcc ggtcattttg aggaacaaaa aaaccagtac
     3301 aaaggtataa agactacgta gtgcaacttc gccaaggaaa tcaaaaggca ctttatcaaa
     3361 tgccatacgg tggagatcga acgccttcat ttttatgctc ttatcgtggt ttatccgcta
     3421 aaagcatagt caacagttca tcggcatggc ggcggtgata gcgcctttga gataaaaccc
     3481 gtattattgc gcgctttccg tacgactaaa gtgattttca tgacgcaaac atttatcccc
     3541 ggcaaagatg ccgctctgga agattccatc gctcgcttcc agcaaaaact ttcagacctc
     3601 ggctttcaga ttgaagaggc ctcctggctg aatcctgtgc ctaacgtctg gtctgtacat
     3661 attcgcgaca aagagtgcgc actgtgtttt accaacggta aaggcgcaac caaaaaagcg
     3721 gcgctggctt ctgcacttgg tgaatatttc gagcgtctct caaccaacta cttttttgct
     3781 gatttctggc tgggcgaaac catcgccaac ggtccgttcg tgcattatcc caacgaaaaa
     3841 tggttcccac tgaccgaaaa tgacgatgtg ccagaaggac tactcgatga ccgtctgcgc
     3901 gcgttttatg atccggagaa tgaactgacc ggcagcatgc tgattgacct acaatccggt
     3961 aacgaagatc gtggtatttg cggcctgccg tttacgcgtc agtccgacaa tcagaccgtt
     4021 tatattccga tgaatatcat tggtaacctg tacgtctcca acggtatgtc cgcaggtaat
     4081 acccgcaacg aagcacgcgt tcagggattg tctgaagttt tcgaacgcta cgtgaaaaac
     4141 cgcattattg ctgaaagcat cagcctgcca gagatcccgg cagacgtgct ggcgcgttac
     4201 ccagcagtag ttgaagccat cgaaacgctg gaagcggagg gtttcccaat cttcgcttat
     4261 gacggttcgc ttggcggcca gtatccggtg atttgcgtgg tactgttcaa tcctgctaac
     4321 ggtacctgct ttgcctcttt cggtgcgcat cctgattttg gcgtagcact ggaacgtacc
     4381 gtgaccgagc tgctgcaagg tcgtggcctg aaagatttgg atgtgtttac tccgccaacc
     4441 ttcgatgatg aagaagtcgc tgaacatacc aacctcgaaa cgcactttat cgattccagc
     4501 ggtttaatct cctgggacct gttcaagcag gatgccgatt atccgtttgt ggactggaat
     4561 ttctccggca ccacggaaga agagtttgct acgctgatgg ctatcttcaa caaagaagat
     4621 aaagaagttt atattgccga ttacgagcat ctgggcgttt atgcttgccg tattatcgtg
     4681 cctggcatgt ccgatattta tccggctgaa gatctgtggc tcgcgaataa cagtatgggc
     4741 agccatttac gtgaaacgat tctttcgcta ccaggcagcg agtgggaaaa agaagattac
     4801 ctgaacctca tcgagcaact ggatgaagaa ggttttgatg actttacccg cgtgcgtgag
     4861 ctgttgggtc tggcgaccgg gtcggataac ggttggtaca ccctgcgtat cggtgaatta
     4921 aaagccatgc tggcgctggc tggtggcgat ctggaacagg ctctggtctg gaccgaatgg
     4981 acgatggagt ttaactcatc agtatttagt ccggaacgcg ccaactatta tcgctgcctg
     5041 caaacgttgt tattactggc acaggaagaa gatcgccagc cgctgcaata tctgaatgcg
     5101 tttgttcgca tgtacggcgc agatgccgta gaagccgcca gtgcggcaat gagcggcgaa
     5161 gcggcgtttt acggcctgca accggtagat agcgatctgc acgcgtttgc tgcacatcag
     5221 tcgttgttga aggcctacga aaagctgcag cgcgccaaag cagcattctg ggcaaaataa
     5281 aatcaaatag cctacgcaat gtaggcttaa tgattagtct gagttatatt acggggcgtt
     5341 ttttt
//
LOCUS       DAZPDU010000242         5300 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZPDU010000242 DAZPDU010000000
VERSION     DAZPDU010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5300
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.246"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            323..409
                     /gene="leuL"
                     /locus_tag="ACHURS_004155"
     CDS             323..409
                     /gene="leuL"
                     /locus_tag="ACHURS_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFR1239752.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            502..2073
                     /gene="leuA"
                     /locus_tag="ACHURS_004156"
     CDS             502..2073
                     /gene="leuA"
                     /locus_tag="ACHURS_004156"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFR1239753.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPLEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSSAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2073..3164
                     /gene="leuB"
                     /locus_tag="ACHURS_004157"
     CDS             2073..3164
                     /gene="leuB"
                     /locus_tag="ACHURS_004157"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFR1239754.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKRF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3167..4567
                     /gene="leuC"
                     /locus_tag="ACHURS_004158"
     CDS             3167..4567
                     /gene="leuC"
                     /locus_tag="ACHURS_004158"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFR1239755.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVKRASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4578..5183
                     /gene="leuD"
                     /locus_tag="ACHURS_004159"
     CDS             4578..5183
                     /gene="leuD"
                     /locus_tag="ACHURS_004159"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFR1239756.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(5232..>5300)
                     /locus_tag="ACHURS_004160"
     CDS             complement(5232..>5300)
                     /locus_tag="ACHURS_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239757.1"
                     /translation="VYWVIAVISVVALFLTAKVKDV"
BASE COUNT         1351 a         1405 c         1398 g         1146 t
ORIGIN      
        1 ttcaccaaat caatatcaaa aaaaatcgca aaacatataa ttcaatacaa atcatcagga
       61 taggttttgc aacgcgtgca ttttgtcccc tttttcctcg ttgattagat gcaaaaattt
      121 atgctgaaat atgtcaaccg atgaaaagcg tcggtagtta agcagaaatt aatatcgctt
      181 actttaacca ccgcagcaca attagctaat tttacggatg cagaactcac gctggcggga
      241 cgtttttatt gcgtcagggt tgacatccgt ttttgtatcc agtaactcta aaagcatatc
      301 gcattcatct ggagctgatt taatgactca catcgttcgc tttatcggtc tactactact
      361 aaacgcatct tctttgcgcg gtagacgagt gagcggcatc cagcattaag ccagcacgca
      421 gtcaaacaaa aaacccgcgc cattgcgcgg gtttttttat gcccgaagcg aggcgctcta
      481 aaagagacaa ggacctaaac catgagccag caagtcatta ttttcgatac cacattgcgc
      541 gacggtgaac aggcgttaca ggcaagcttg agtgtgaaag aaaaactgca aattgcgctg
      601 gcccttgagc gtatgggtgt tgacgtgatg gaagtcggtt tccccgtctc ttcgccgggc
      661 gattttgaat cggtgcaaac catcgcccgc caggttaaaa acagccgcgt atgtgcgtta
      721 gctcgctgcg tggaaaaaga tatcgacgtg gcggccgaat ccctgaaagt cgccgaagcc
      781 ttccgtattc atacctttat tgctacctcc ccaatgcaca tcgccaccaa gctgcgcagc
      841 accctggacg aggtgatcga acgcgctatc tatatggtga aacgcgcccg taattacacc
      901 gatgatgttg aattttcttg cgaagatgcc gggcgtacac ccattgccga tctggcgcga
      961 gtggtcgaag cggcgattaa tgccggtgcc accaccatca acattccgga caccgtgggc
     1021 tacaccatgc cgcttgagtt cgccggaatc atcagcggcc tgtatgaacg cgtacctaac
     1081 atcgacaaag ccattatttc cgtacatacc cacgacgatt taggcctggc ggtcggcaac
     1141 tcactggcgg cggtacatgc cggtgcacgc caggtggaag gtgcaatgaa cgggatcggc
     1201 gagcgtgccg gtaactgttc gctggaagaa gtcatcatgg cgattaaagt tcgtaaggat
     1261 attctcaacg tccacaccgc cattaatcac caggagatat ggcgcaccag ccagttagtt
     1321 agccagattt gtaatatgcc gatcccggca aacaaagcca ttgttggcag cagcgcattc
     1381 gctcactcct ccggtatcca ccaggatggc gtgctgaaaa accgcgaaaa ctacgaaatc
     1441 atgacaccag aatctattgg tctgaaccaa atccagctga atctgacctc tcgttcgggg
     1501 cgtgcggcgg tgaaacatcg catggatgag atggggtata aagaaagtga atataattta
     1561 gacaatttgt acgatgcttt cctgaagctg gcggacaaaa aaggtcaggt gtttgattac
     1621 gatctggagg cgctggcctt catcggtaag cagcaagaag agccggagca tttccgtctg
     1681 gattacttca gcgtgcagtc tggctctaac gatatcgcca ccgccgccgt caaactggcc
     1741 tgtggcgaag aagtcaaagc agaagccgcc aacggtaacg gtccggtcga tgccgtctat
     1801 caggcaatta accgcatcac tgactataac gtcgaactgg tgaaatacag cctgaccgcc
     1861 aaaggtcacg gtaaagatgc gctgggtcag gtggatattg tcgccaacta caacggtcgc
     1921 cgcttccacg gcgtcggcct ggccaccgat attgtcgagt cctccgccaa agccatggtg
     1981 cacgtactga acaatatctg gcgtgccgca gaagtcgaaa aagagttgca acgcaaagct
     2041 caacacaacg aaaacaacaa ggaaaccgtg tgatgtcgaa gaattaccat attgccgtat
     2101 tgccggggga cggtattggt ccggaagtga tgacccaggc gctgaaagtg ctgaatgccg
     2161 tgcgcaaccg ctttgcgatg cgcatcacca ccagccatta cgatgtaggc ggcgcagcca
     2221 ttgataacca cgggcaacca ctgccgcctg cgacggttga aggttgtgag caagccgatg
     2281 ccgtgctgtt tggctcggta ggcggcccga agtgggaaca tttaccacca gaccagcaac
     2341 cagaacgcgg cgcgctgctg cctctgcgta agcgcttcaa attattcagc aacctgcgcc
     2401 cggcaaaact gtatcagggg ctggaagcat tctgtccgct gcgtgcagac attgccgcta
     2461 acggcttcga catcctgtgc gtgcgcgaac tgaccggcgg catctatttc ggtcagccaa
     2521 aaggccgcga aggtagcgga caatatgaaa aagcgtttga taccgaggtg tatcaccgtt
     2581 ttgagatcga gcgtattgcc cgcatcgcgt ttgaatctgc tcgtaaacgt cgccacaaag
     2641 tcacctcaat cgacaaagct aacgtgctgc aatcctctat tttatggcgt gaaatcgtca
     2701 acgagatcgc cacggaatac ccggatgtcg aactggcgca tatgtacatc gacaacgcca
     2761 ccatgcagct gattaaagat ccatcacagt ttgacgttct gctgtgctcc aacctgtttg
     2821 gcgacattct gtctgacgag tgcgcaatga tcactggctc gatggggatg ttgccttccg
     2881 ccagcctgaa cgagcaaggt tttggtctgt atgaaccggc aggcggctca gcaccagata
     2941 tcgcaggcaa aaacatcgcc aacccgattg cgcaaattct gtcgctggca ctgctgctgc
     3001 gctacagcct ggatgccgat gatgcggctt gcgccattga acgcgccatt aaccgcgcat
     3061 tagaagaagg cattcgcacc ggggatttag cccgtggcgc tgccgccgtt agtaccgatg
     3121 aaatgggcga tatcattgcc cgctatgtgg cagaaggggt gtaatcatgg ctaaaacgtt
     3181 atacgaaaaa ttattcgacg ctcacgtagt gtacgaagcc gaaaacgaaa ccccgctgtt
     3241 atatatcgac cgccacctgg tgcatgaagt gacctcaccg caggcgttcg atggtctgcg
     3301 cgcccacggt cgcccggtac gtcagccggg caaaaccttc gccactatgg atcacaacgt
     3361 ctctacccag acgaaagaca ttaatgcctg tggtgaaatg gcgcgcatcc agatgcagga
     3421 gctgattaaa aactgcaaag aatttggcgt cgaactgtat gacctgaatc acccgtatca
     3481 ggggatcgtc cacgtaatgg ggccggaaca gggcgtcacc ttgccgggga tgaccattgt
     3541 ctgcggcgac tcgcataccg ccacccacgg cgcgtttggc gcactggcct ttggtatcgg
     3601 cacttccgaa gttgaacacg tactggcaac gcaaaccctg aaacagggcc gcgcaaaaac
     3661 catgaaaatt gaagtccagg gcaaagccgc gccgggcatt accgcaaaag atatcgtgct
     3721 ggcaattatc ggtaaaaccg gtagcgcagg cggcaccggg catgtggtgg agttttgcgg
     3781 cgaagcaatc cgtgatttaa gcatggaagg tcgtatgacc ctgtgcaata tggcaatcga
     3841 aatgggcgca aaagccggtc tggttgcacc ggacgaaacc acctttaact atgtcaaagg
     3901 ccgtttgcat gcgccgaaag gcaaagattt cgacgacgcc gttgcctact ggaaaaccct
     3961 gcaaaccgac gaaggcgcaa ctttcgatac cgttgtcact ctgcaagcag aagaaatttc
     4021 accgcaggtc acctggggca ccaatccagg ccaggtgatt tccgtgaacg acaatattcc
     4081 cgatccggct tcgtttgccg atccggttaa acgcgcgtcg gcagaaaaag cgctggccta
     4141 tatggggctg aaaccgggca ttccgctgac cgaagtggct atcgacaaag tgtttatcgg
     4201 ttcctgtacc aactcgcgca ttgaagattt acgcgcggca gcggagatcg ccaaagggcg
     4261 aaaagtcgcg ccaggcgtgc aggcactggt ggttcccggc tctggcccgg taaaagccca
     4321 ggcggaagcg gaaggtctgg ataaaatctt tattgaagcc ggttttgaat ggcgcttgcc
     4381 tggctgctca atgtgtctgg cgatgaacaa cgaccgtctg aatccgggcg aacgttgtgc
     4441 atccaccagc aaccgtaact ttgaaggccg ccaggggcgc ggcgggcgca cgcatctggt
     4501 cagcccggca atggctgccg ctgctgctgt gaccggacat ttcgccgaca ttcgcaacat
     4561 taaataagga gcacaccatg gcagagaaat ttatcaaaca cacaggcctg gtggttccgc
     4621 tggatgccgc caatgtcgat accgatgcaa tcatcccgaa acagtttttg cagaaagtga
     4681 cccgtacggg ttttggcgcg catctgttta acgactggcg ttttctcgat gaaaaaggcc
     4741 aacagccaaa cccggacttc gtgctgaact tcccgcagta tcagggcgct tccattttgc
     4801 tggcacgaga aaacttcggc tgtggctcct cgcgtgagca cgcgccctgg gcattgaccg
     4861 actacggctt taaagtggtg attgcgccga gttttgctga catcttctac ggcaatagct
     4921 ttaacaacca gctgctgccg gtgaaattaa gcgatgcaga agtggacgaa ctgtttgcgc
     4981 tggtgaaagc caatccgggg atccatttcg acgtggatct ggaagcgcaa gaggtgaaag
     5041 cgggagagaa aacctatcgc tttaccatcg atgccttccg ccgccactgc atgatgaacg
     5101 gtctggacag tattgggctt accttgcagc acgacgacgc cattgccgct tatgaagcaa
     5161 aacaacctgc gtttatgaat taatcccctt gcccggtcaa atgaccgggc tttccgctat
     5221 cgtccacgtc atcaaacgtc tttaaccttt gcggttaaaa ataatgcgac aacagaaata
     5281 accgcaatta cccagtagac
//
LOCUS       DAZPDU010000243         5189 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZPDU010000243 DAZPDU010000000
VERSION     DAZPDU010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5189
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.247"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..428
                     /gene="soxR"
                     /locus_tag="ACHURS_004161"
     CDS             <1..428
                     /gene="soxR"
                     /locus_tag="ACHURS_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFR1239758.1"
                     /translation="PGEVAKRSGVAVSALHFYESKGLITSIRNSGNQRRYKRDVLRYV
                     AIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREELDRRIHTLVALRDE
                     LDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            974..2323
                     /gene="ghxP"
                     /locus_tag="ACHURS_004162"
                     /gene_synonym="yjcD"
     CDS             974..2323
                     /gene="ghxP"
                     /locus_tag="ACHURS_004162"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFR1239759.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            2475..4124
                     /locus_tag="ACHURS_004163"
     CDS             2475..4124
                     /locus_tag="ACHURS_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFR1239760.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(4278..5189)
                     /locus_tag="ACHURS_004164"
                     /pseudo
     CDS             complement(4278..5189)
                     /locus_tag="ACHURS_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
BASE COUNT         1124 a         1206 c         1398 g         1461 t
ORIGIN      
        1 cccccggcga agtggcgaaa cgcagcggtg tggcggtatc ggcgctgcat ttctatgaaa
       61 gtaaagggtt gattaccagt atccgtaaca gcggcaatca gcggcgatat aaacgtgatg
      121 tgttgcgata tgttgcaatt atcaaaattg ctcagcgtat tggcattccg ctggcgacca
      181 ttggtgaagc gtttggcgtg ttgcccgaag ggcatacgtt aagtgcgaaa gagtggaaac
      241 agctttcgtc ccaatggcga gaagagttgg atcggcgcat tcatacctta gtggcgctgc
      301 gtgacgaact ggacggatgt attggttgtg gctgcctttc gcgcagtgat tgcccgttgc
      361 gtaacccggg cgaccgctta ggagaagaag gtaccggcgc acgcttgctg gaagatgaac
      421 aaaactaaag cgccacaagg gcgctttagt ttgttttccg gtctttgtct ttctctctat
      481 cccgctggta cacaggaggg tttcccccga cgtcaacaca ccccattcga gcacgtggtg
      541 gaggttccgg ttggtgttga tgctttaatt gtatgtcacc ggcatttctt cgccagtgta
      601 aaagtatact ttttatccgc aatatttttg tcatctcaga cgatttttta tcgcaatcct
      661 gaacggtata cggctcgata acgctgcaat cttgcgcacc gacgataacg tttgcgcatc
      721 aattgcctgg tttttcatcg tcaagacaat aaaagagaaa aaagcagcaa acttcggttg
      781 aaaaagccgc tatgatcgcc ggataatcgt ttgctttttt taccacccgt tttgtatgcg
      841 cggagctaaa cgtttgcttt tttgcgacgc agtaaattgt cgcaaacctg gagcaggaag
      901 ataacgtttc gctggcaggg gattgtccgc cacgcatctt gacgaaaatt aaactctcag
      961 gggatgtttt cttatgtcta cgccatcagc gcgtaccggc ggttcactcg acgcctggtt
     1021 taaaatttca caacgtggaa gcactgtccg tcaggaagtg gttgccgggt taacaacgtt
     1081 tctggcgatg gtctactcgg tcatcgtcgt tccaggtatg ttgggtaaag caggcttccc
     1141 gcctgcggca gttttcgttg caacctgtct ggttgccgga ctcggttcta tcgtgatggg
     1201 tctgtgggct aatctgccgt tggcgattgg ttgcgccatc tccctgacgg cgtttaccgc
     1261 attcagcctg gtgctggggc aacatattag cgtacctgtc gcgctgggtg ccgtgttcct
     1321 gatgggtgtg ctgtttacgg taatttctgc cacgggtatc cgtagctgga ttttgcgcaa
     1381 cttgccgcac ggtgtggcgc acggcacggg gatcggtatc ggtctgttcc tgctgctcat
     1441 tgccgctaat ggtgtcggtc tggtgattaa aaacccgctt gatggtctgc ccgttgcgct
     1501 gggtgatttc gcgaccttcc cggtgattat gtcactggta ggtctggcgg tgatcatcgg
     1561 cctggaaaaa ctgaaagtcc ctggtggcat tctgctgacc attatcggta tctcaattgt
     1621 cggtttgatc ttcgatccta acgtccattt ctccggcgtt ttcgccatgc cttcattgag
     1681 cgatgaaaac ggcaattcac tgattggcag cctggacatt atgggcgcgc tgaatcctgt
     1741 agtcctgcca agcgttctgg cgctggtgat gacggcagta tttgatgcca ccggaactat
     1801 ccgtgccgtc gccggccagg cgaacctgct ggataaagat gggcagatca tcgacggtgg
     1861 gaaagcactg accactgact ccatgagcag cgttttctct ggcctggtgg gtgctgctcc
     1921 ggcagcggta tacatcgagt ctgcggcggg tacggcggcg ggcggtaaaa ccggtttgac
     1981 ggctatcacc gttggcgtgc tgttcctgct gattctgttc ctctctccgc tctcttacct
     2041 cgttccgggg tatgcaacgg ctccggcgct gatgtacgtt ggcctgctga tgctgagcaa
     2101 cgtggcgaaa atcgactttg ctgattttgt tgatgcgatg gcgggtctgg ttacggcggt
     2161 attcatcgtg ctgacctgta acatcgtaac aggcatcatg atcggcttcg cgactctggt
     2221 gattggtcgt ctggtttccg gcgaatggcg caagttgaac atcggtacgg tcgttatcgc
     2281 cgtggcgctg gtgaccttct atgcgggtgg ctgggctatc taatctttca tcggattctg
     2341 aaaacgggtg gcaatggctg cccgttttta ttttctccgc acattgtgtg agcttttgcg
     2401 atattctgaa aaaatgagaa ttcaggcata acgcctttct cagatcataa aaaaacatcg
     2461 caaacaggga acgcatggaa atcttcttca ccatactgat aatgaccctc gtggtctcac
     2521 tgtccggggt ggtcactcgt gtcatgccct ttcagatccc gcttccgctt atgcaaatcg
     2581 ccatcggtgc gctactggcg tggccgacgt ttggtttgca tgtggagttt gatcctgaac
     2641 tctttttagt cttgtttatc ccgccgttgc tgttcgctga tggctggaaa acgccgaccc
     2701 gtgaatttct cgaacatggt cgagagattt tcggcctcgc gctggcgctg gtggtggtca
     2761 ccgtggtcgg cattggcttc cttatttact gggtggtgcc gggcattccg ctgatccccg
     2821 cctttgcgct ggcggcggta ctttctccga ccgatgctgt ggcgctctcc gggattgttg
     2881 gcgaagggcg catcccgaaa aaaatcatgg gcattttgca gggcgaagcg ttgatgaatg
     2941 acgcctccgg cctggtgtcg ttgaagtttg ccgtggcagt ggcgatgggg acgatgatct
     3001 tcaccgtcgg cggtgcgacg gtcgaattta tgaaagtagc cattggtggt attcttgccg
     3061 gttttgtggt gagctggctg tatggtcgct cgctgcgatt cctcagccgc tggggcggtg
     3121 atgaacccgc gacgcagatc gttctgctgt tcttgctgcc attcgcttct tatctgattg
     3181 ccgaacatat tggcgtttcc ggcatcctcg ctgcggttgc cgccgggatg accatcaccc
     3241 gctccggtgt gatgcgccgt gcgccgctgg caatgcgcct gcgcgcaaac agcacctggg
     3301 cgatgctgga atttgtcttt aacggcatgg tgttcctgct gttaggtctg cagctgccgg
     3361 gtattctgga gacgtcgctg atggcggcag aaatcgaccc taacgtcgaa atctggatgc
     3421 tgtttaccga tattattctg atatatgcgg cgctgatgct ggtccgtttc ggctggctgt
     3481 ggacgatgaa aaagttcagc aaccgcttcc tgaagaagaa gccgatggag tttggttcgt
     3541 ggaccacacg agaaatcctg atcgcgtctt tcgccggggt gcgtggggcg atcactctgg
     3601 ccggtgtgct ctctatcccg ctgctcttgc cggatggtaa cgtcttcccg gcgcgctatg
     3661 agctggtgtt cctggcggct ggcgtcattc tcttctcgct gtttgtcggc gtggtgatgt
     3721 tgcctattct gctacaacac attgaagtcg cggaccattc gcaacaattg aaagaagaac
     3781 gtattgcgcg agcggcaacg gcagaagtgg cgattgtggc gatccagaaa atggaggagc
     3841 gtctggcggc ggataccgaa gagaatatcg ataaccagct gcttacggag gtcagttctc
     3901 gcgtcattgg taacctgcgt cgtcgcgccg atggacgtaa cgacgttgaa agttccgtgc
     3961 aggaagagaa ccttgagcgt cgcttccgtc tggcggcatt gcgttctgaa cgtgctgaac
     4021 tgtaccacct gcgcgccacg cgggagatca gcaacgaaac gctgcaaaaa ttactgcacg
     4081 atctcgattt gcttgaagcg ttgctaattg aggaaaatca gtaagccgaa agcgacgtaa
     4141 ccaggcccga ggtaatctga aaaaggtgca atcttttgca cctttaacag gttagctcgg
     4201 gctaacattc atgattctaa aacaaataag ttatctctct tgtaaagtca attcattggc
     4261 tgttcttcga cttaacctca atctgcgtgt tgcataatat ctgcagtaat cttcatagcg
     4321 actgcattaa tattatcctc agtttgtgac atgccagtat gagaggtaca atcttcttca
     4381 aagaatttct gtagtgttac caaggtatca ctatcaatgg ggatattttc ctccccacgg
     4441 agtatggaaa tgcccattcc ctggacgcta gcctcatcaa aattaacccg atagagacgg
     4501 gtattaaaca tacaggcttt tgttaaaata gaacagttga aatggcagtt gcttaaattg
     4561 gcattattaa aattaacatt agccatatta gagtcggtac atataaccat ttcgaggaat
     4621 gtatttgtta gccacgtaca aaagagtttg ctatcaatta agtttatatt ctggaggatt
     4681 gctttgttca gattgacgcg agacagatca gcatgttcaa agttaattaa atcgagttta
     4741 gagaaactaa ggtcggcttt aaataaatta acatatggcg ctattacttt ataaagattt
     4801 gcctcagcca tgtacgccat ataaaaattt gaatagtcaa gaaaggtatt tttaatattg
     4861 gctctattca ttgctgcatt gtagaattga acatgcataa cggagcatgc atttagagtt
     4921 ccgtcggcca tttttatctg agcgaaattt gagtcatcga gaatacaatt atcaaaaagt
     4981 atttttccat tatggcgtaa taaaaattcg ttttatacat attacagtcc tggaaaatag
     5041 cgttttgtag tctgcaacct gcaaaattcg aattggataa atcttcatct ttaatcacga
     5101 tacctttcag gctaaggtgt gaaaaatcgt aaccaccatt tactgtctgt ggaatttcga
     5161 tagtattatt atggacggcg acattattt
//
LOCUS       DAZPDU010000244         5176 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZPDU010000244 DAZPDU010000000
VERSION     DAZPDU010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5176
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.248"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(15..773)
                     /gene="aroD"
                     /locus_tag="ACHURS_004165"
     CDS             complement(15..773)
                     /gene="aroD"
                     /locus_tag="ACHURS_004165"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFR1239761.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(804..1670)
                     /gene="ydiB"
                     /locus_tag="ACHURS_004166"
     CDS             complement(804..1670)
                     /gene="ydiB"
                     /locus_tag="ACHURS_004166"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFR1239762.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEVLASADILTNGTKVGMNPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1682..2947)
                     /locus_tag="ACHURS_004167"
     CDS             complement(1682..2947)
                     /locus_tag="ACHURS_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1239763.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFSRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(3174..4388)
                     /locus_tag="ACHURS_004168"
     CDS             complement(3174..4388)
                     /locus_tag="ACHURS_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1239764.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(4488..4844)
                     /locus_tag="ACHURS_004169"
     CDS             complement(4488..4844)
                     /locus_tag="ACHURS_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFR1239765.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4980..5087)
                     /gene="rprA"
                     /locus_tag="ACHURS_004170"
     ncRNA           complement(4980..5087)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHURS_004170"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
BASE COUNT         1518 a         1185 c         1170 g         1303 t
ORIGIN      
        1 cgaaatatta ttgcttatgc ctggtgtaaa atagttaata ccgtgcgtaa atcatttacc
       61 gagatttgcc ctggtgcaga ggcttttttt accgcaccaa aagttgccgc cgagccaaat
      121 acttcaccag ccagacgaga aattacgcca gtttttgcca tcgacatcgt gatgattgga
      181 cgatcggcat actgctcctg catctccagg gtcgcggtaa gcaacgtcag cacatcgctg
      241 gtactttgcg gcatcagcgc aatcttagga atatcggcgt cgaaggactg cattttgcgc
      301 agacgggcaa tgatttcttc ggcttccggc gttttatgga agtcatggtt ggacatgaca
      361 actttcacat catgcgcgtg ggcgtaggcg acggtttctt taacctgatc atcgccggta
      421 aataactcca gatcgatcat atcaaccagg ccgctgtcga tggctgcacg attgagagca
      481 atataagcct cggtggaaat cgcctgctcg ccgccttctt tggcactgcg gaaggtaaac
      541 agcagcggtt tttctggcat ggtttcacgg agaatttttg ccgccgccat gacagactcc
      601 acattggaga ggtcggcaaa gtggtccaca cgccattcca gaatatcaaa gtccgcttca
      661 cgataggcga gagcttcgga tttcacgcgg gcgatatctt tcgccatcag cgagacgatg
      721 attttaggtg cgcccgcacc aatgacgaga tcttttacag ttacggtttt cattttttac
      781 cctttctgca cgcggtcagc ctgtcaggca ccgaacccca tgacctgttt aacatattcc
      841 agagggaaat ctttgccagt ccataacgtg aactgttcag ccccttgcca caacaacatg
      901 ccgtatccat caatcgtttt gcaacctgct tgttgtgcct gctgcaataa cttcgtcata
      961 tgcgggttat acacgcattc agtgaccagc agtcccggat gtaacagact gatatcatta
     1021 accaatgatt cattctcaag ggggttcata cctacttttg tgccattggt taaaatgtcg
     1081 gcggaagcca ggacttcagc aaaggcttgc tgatcggcga gatcggtaac cgtgacgaca
     1141 caatcggtgt tttcgttaac ccgctgcgcg aaggcgaggg ctttatcgaa gaactcatcc
     1201 cgacggttaa agagtttaat ttcttttaaa ccttcaattg ccccctgcgc gccaatcgct
     1261 gttgaggcac ctccggcccc taacagcacc atcgttttgc ctttgatatc aaaaccgctc
     1321 tctttaatgg cgcgaatatg gcccgtgccg tcggtgttat agccacgcag atagccatca
     1381 tcattaacga tggtgttgat ggcacccacc agtttggctg ctggcgttaa ttcatcaaca
     1441 tattcacacg ccagttgttt gttcggcatc gacacaccag ttccgcgcat tttgagggct
     1501 tttaatcctt caattgctcc gggaaagcta tcgttatcca cttcgaaggc catataggta
     1561 aatggcaatc ccgctttttc taaggcttta ttctgcattt caggcgataa actgtggcgg
     1621 ataggataag ccatcaaccc aatcaattcg tattttgcgg taacatccat aattaactcc
     1681 tttaacctct atgcttaatt gtgtttaacc gggtgctaaa tttgcgttcg ccaaaccgca
     1741 catcattctc aggaataata aaaacgcggt aatagcggct aaaaacaata attgcggtaa
     1801 tcagggccag cagcgcgaaa gtaaaatcga gaacaatgat atattgcagg ccgatgttcg
     1861 acagataacc ggtaatcagc ggaataacaa agttagccag tccacccatc atcatataaa
     1921 tactggtgac tttggctttg cttttgggga aaaactctga catgaccgaa acaccgagct
     1981 gtaaaatgcc gccagctgct gagaaaccga taacaaaggc gccggcatta cacaccagtg
     2041 gagaagggta caggtagata atggcagctg ttattgttgc cagcgcagag ttaaatacat
     2101 tagcccagat gggccggacc atttttttca gtagtgcggc aaaaataaag acacagacca
     2161 acgagcccat actgtaataa gagatggttt ttaatgcctc agcttctgac ataccagcaa
     2221 aagccatcgc atatttgggc atccacacca caatcacata aaaggtcgag aatgcggcta
     2281 caccgaacag taccgatgaa acaccttcca gccagactaa cggtttgctg ttcatttgcg
     2341 gtaattcatt agctacgctg gcgtccacca actggctggg gaatttgctt ttcaacagca
     2401 tcagcgtgat cagtacaaac agaatacccg gaataatcag cccatagccg taccagatat
     2461 tattgagcaa catatagctc accagcattg ggtagaacat ttgcccaaat gacaccatcg
     2521 ctttaaccag tatgaccgcc gaaccagagg ctttcggaaa gcattccatg agcgcggggt
     2581 agccacccgt atccagcgct gagttagcga tacctacgca cactgccaga ccgtaggcga
     2641 gagttaaatt cgggcaagcg ggaataccaa agaagaatag cagatacatt attactgcca
     2701 ttaatatcac cgcccgacga ccaaacttat cggagatcac accgaagaat aaaatactga
     2761 tcaatcgccc caaaccgata ccggaaatta aataggcaat gcccgcgttg tcagtggaaa
     2821 acttttctgc cagagatgtc atattttggg caagcgtaat aacactaatg ccgtgcagga
     2881 aatagctgaa gtaaatacaa agaacagcca ggataaatgg cgtgctgaaa gccttatttt
     2941 gagacatttt cacagctcct tgctgaaaga taatttcgtt accgtacgta ttgttctccg
     3001 acggtttaca taattactat actgaagatg attttccctg ggtacatggc ataaagttta
     3061 tgtttccagg aatattgcat gtcatagttt gtttttgatc gcgattttcc ttagttagcg
     3121 cagaaagagt tatttatccg taagatcttg catgacctga aaagaatatt gcattaccca
     3181 cccggagcga cattttcctt tagcgagtga tgccgcgttt ttttgcggct gcgtaagccg
     3241 ataaacagtg ccagtaaaaa accgatggca gcgatggcgg tatcgaacca cataatatcg
     3301 gcaatacttc tttgggacag atgagccgta atcaacggaa tagtaaaggt cgcaatactg
     3361 cccgcactgt aatagatccc tgtagcttta cctttagcgt aagggaaacg ttcagccatt
     3421 aacgtcaggc caatttgcac aacacctcca gcagaggtaa aaccaatgac aaaagcaaat
     3481 attatcacca cataaaatgt gggatgcagg cagacggtaa acagagcaat aaatgagata
     3541 aaggtgtaca gcatcagtaa tgttgttggg cgaacggtat tacgaatgag tggagcggta
     3601 ataaatacac aaagcagcga acccacggtg tagatactga gtagtttgat cgacatagtg
     3661 tatgacatgc ctgcaacaaa ttgtccgtac tgtgccagcc actggctaac cagataaaac
     3721 gttgccattg agatatagcc atataaggta taactggcta aatcgataat tgaacagcga
     3781 tgttccgtag agctggtggt tttctttatg acaggtaagc gacgacccgg atggggtggg
     3841 aacgtacaac ggtataaaaa cagagcgtta ataaacataa tgcctgcagc aatcataaag
     3901 gaccaaccga accacagttc agcccacact aacaggctaa tgattagcgg taataaaaat
     3961 tgtccgctgg aaacaaatgc tttaattaaa atattggctg tcccaggtga gcgtggaaaa
     4021 gcttccatca aactgggata agtgcctgca tcgagaaaac tgtttgccat tcccgccaga
     4081 aagccaaaaa cataagcgat aatgatgtta ttggtctgca ggatgccaaa aaagaaggcc
     4141 atatagcagc acatcccgag catgataaaa gggcggcgac caaagcgatc ggataataat
     4201 cctgcaaaaa gcaagacact taatcgacca atgcccagcg atgagataac tatcgagaca
     4261 cccgcggcat tagtctgcca aagtgtctcc agcgaggcca tattcaggct catcaaaatg
     4321 acgcccatac catgcaccag gtaattaaaa tacaacccaa gtgcggtagg gtaataggga
     4381 tttttcatag gttctactta gcaaaggtgc tttaaaaagt gtagcgatgt tgtgtcagta
     4441 tccattcatt attgttgtaa caacgacaat tcctttctgt ggaaaaatta acaaagccgc
     4501 tccagatcgt gtgcatataa ttcagcggct accgattgat agatcttcca gtcaataaaa
     4561 ttgccatcag ggtagacttg ttgaaacgcg gttaagtcgg ggaaaaaacg catggtgttg
     4621 ttgccgatgc ggttatttat tttctctatg atggcatgaa gatgtttttt tcgttgctga
     4681 cgcatagcca acagttgctc gactacacaa tcaggaatgg cgcatttgcc attttcccac
     4741 tgacgccagc tttcactgtc accggtctgg gcaatccagg tcgcgcattc atcaatagtc
     4801 atggcaaaaa tatggcggag tgcctggagt tcataagcgt tcatcgtgta atggctgcat
     4861 gaaataaaca ataaaatata aataacatta tcagcctgct gacggcttga agagagtcac
     4921 agtatcttgt gcaacattat tggaaagagt gaggggcgag gtagcgaagc ggaaaaatgt
     4981 taaaaaaaag cccatcgtgg gagatgggca aagactacac acagcaattc gttgtttcac
     5041 tcaggggatt tccatgctta taaatcaata tgttgattta taaccgtgag ctaatagtag
     5101 gcatacggac tttttgcgtc gatcagattc gtctcattag tttaaaaact gtaaagaatt
     5161 tgcgacaggt actacc
//
LOCUS       DAZPDU010000245         5085 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZPDU010000245 DAZPDU010000000
VERSION     DAZPDU010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5085
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.249"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..489
                     /locus_tag="ACHURS_004171"
     CDS             <1..489
                     /locus_tag="ACHURS_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1239766.1"
                     /translation="VNVRRNLTILDMFGPPKTNAGIRTVTLLQPALEALKEQYKLTGH
                     HRKSEITFYHREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGARWNAAVKRAGIRR
                     RNPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNAR
                     LS"
     gene            541..1476
                     /gene="ttcA"
                     /locus_tag="ACHURS_004172"
     CDS             541..1476
                     /gene="ttcA"
                     /locus_tag="ACHURS_004172"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFR1239767.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(1605..2978)
                     /gene="dbpA"
                     /locus_tag="ACHURS_004173"
     CDS             complement(1605..2978)
                     /gene="dbpA"
                     /locus_tag="ACHURS_004173"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFR1239768.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAVISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     SRVRLLK"
     gene            complement(3008..3181)
                     /gene="ynaL"
                     /locus_tag="ACHURS_004174"
     CDS             complement(3008..3181)
                     /gene="ynaL"
                     /locus_tag="ACHURS_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFR1239769.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(3456..4439)
                     /gene="zntB"
                     /locus_tag="ACHURS_004175"
     CDS             complement(3456..4439)
                     /gene="zntB"
                     /locus_tag="ACHURS_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFR1239770.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(4453..4500)
                     /gene="ydaN"
                     /locus_tag="ACHURS_004176"
     CDS             complement(4453..4500)
                     /gene="ydaN"
                     /locus_tag="ACHURS_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFR1239771.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            4694..>5085
                     /gene="dgcM"
                     /locus_tag="ACHURS_004177"
     CDS             4694..>5085
                     /gene="dgcM"
                     /locus_tag="ACHURS_004177"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFR1239772.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALNCRLVLRKLTEAEPVI
                     IFEGIEASATLGLKASRSANYQRKKQGF"
BASE COUNT         1382 a         1297 c         1211 g         1195 t
ORIGIN      
        1 gtgaatgtca gaagaaacct gacgatactt gatatgttcg gtcccccaaa aacaaatgcc
       61 gggatccgga cggtaacatt actgcagccg gctcttgaag cactgaagga gcaatacaaa
      121 ctgaccgggc atcatcgcaa aagcgaaatc actttttatc atcgggagta cggcagaact
      181 gaaaagcaaa aactgcattt tgttttcatg cccagggtgt gtaacgaaaa acagaaacct
      241 tattactcgg taagcagttt gggtgcaaga tggaatgcag cagtaaaacg tgctggtatt
      301 cgccgccgta atccgtacca tacgcgacat acttttgcct gctggctgtt gacggcagga
      361 gcgaacccgg catttatagc cagccagatg gggcatgaaa ctgcgcaaat ggtgtatgaa
      421 atttacggta tgtggattga tgacatgaac gacgaacaga tagccatgtt gaatgcgcgg
      481 ttatcgtagt tgcaaagttt gcccccaatt tgccccattt agtaccagag aactgaaata
      541 atgcaagaaa atcaacaaat tacaaagaaa gaacaataca acctgaacaa attacaaaaa
      601 cgtctgcgtc gtaacgtggg cgaagccatt gctgacttca atatgattga agaaggcgat
      661 cgcatcatgg tttgcctctc cgggggtaaa gacagctata ccatgctgga gattctgcgc
      721 aatttgcagc aaagcgcgcc aatcaatttt tcgctggtgg ctgttaacct cgatcaaaag
      781 caaccgggct tcccggaaca cgttctgccc gagtatcttg aaaagctggg cgttgagtac
      841 aagattgtcg aagagaacac ttacggtatc gtgaaagaga agattccaga gggcaaaacc
      901 acttgctcac tgtgttctcg ccttcgtcgc ggtatccttt atcgtaccgc aacggaactg
      961 ggggcgacga agatcgcgct gggtcaccat cgtgacgata tcctgcagac gctgttctta
     1021 aatatgttct acggcggcaa gatgaaaggt atgcctccga aactgatgag cgatgatggc
     1081 aaacatatcg ttattcgtcc gctggcctac tgccgcgaga aagatattca gcgatttgcc
     1141 gatgcaaaag cgttcccgat tattccgtgc aacctgtgcg gttcacagcc taacctgcaa
     1201 cgtcaggtga ttgctgacat gttgcgtgac tgggataaac gttatccagg gcgtatcgag
     1261 acgatgttca gcgcgatgca gaatgtggtg ccgtcgcatc tgtgcgatac caacctgttc
     1321 gatttcaaag gcatcaccca cggttctgaa gtggttaacg ggggtgatct ggcgtttgat
     1381 cgcgaagaga tcccactaca accggcgggc tggcagccag aagaagatga aaatcagttg
     1441 gatgagttac ggctgaatgt ggttgaagtg aaataaccag gatggcgccc gatgcgcaag
     1501 cgtatcgggc tactcttatg gaggccggat aagacgcggc cagcgtcgca tccggcaatc
     1561 ccgaataaga tgtttactct tgcacccggc aattcaacat ttcattattt taataaccgc
     1621 acccggctcg tttttccttt aatcttcccg ccctgtaact gtttccatgc tttatgagca
     1681 acagcctgac ggaccgcgac atagacatgc gccggatgca cggcgatttt gccaatatct
     1741 gcgccatcaa gcccgatatc tcctgtcagt gcacctaata catcacccgg gcgcattttg
     1801 gcttttttcc caccatcgat acacaacgtt gccatttctg cttccagcgg cacaatggaa
     1861 ctattagctg gcggcgtttg ccagttaagt tttatctgca acatgtcaga aatgatattg
     1921 gcccgctgtg cttcttccgg agcacagaaa ctgatcgcca gaccgctatt tcctgcacga
     1981 gctgtacggc cgatgcgatg tacatgaact tcagggtccc acgccagctc aaagttcacc
     2041 accagctcaa gcgatttaat atccagacca cgcgcagcaa catcagtcgc aaccagtaca
     2101 cgggcgctac cgttagcaaa acgtaccagg gtctgatcgc gatcgcgttg ctccaaatcg
     2161 ccgtgtaatg acaatgcact ttgccctact tcattcagcg cgtcgcagac agcctggcaa
     2221 tcttttttgg tattgcaaaa caccacgcaa gaggatggct gatgcaagct taataaccgt
     2281 tgcaacagag gaattttgcc tttgctggat gtctcataaa attgttgttc aatgggtggc
     2341 aaagcatctg ttgagtcaat ttcaatcgcc aaaggatcgc gttgcactcg tccgctgatt
     2401 acggcaatgg cttccggcca ggttgccgaa aacagaagcg tctgtcgaga tgcaggcgca
     2461 aaacggatga catcatcaat ggcatcgcta aatcccatat ccagcatgcg gtcggcctca
     2521 tccatcacca aagtattcag cgcatccagt gataccgtgc ctttttgcaa gtgatccagc
     2581 aaacgacccg gcgttgccac gataatatgc ggcgcatgtt gcaacgaatc acgctgcata
     2641 ccgaacggtt gaccaccgca caacgtcaaa attttggtat ttggcagaaa acgcgccagc
     2701 cgacgcaatt cacctgccac ctgatccgcc agttcacgcg taggacacag cactaaagcc
     2761 tgggtttgaa atagcgacgc atcaatttgc tgtaacaagc cgaggccaaa agccgccgtt
     2821 ttgccgctgc cggttttcgc ctgcacgcga acatcttttc cggcaaggat cgccggaagc
     2881 gcggcggcct gcaccggcgt catagttaaa taacccaact cattaaggtt cgtgagttgg
     2941 gcgggaggca aaacattcag ggtagaaaaa gcggtcacaa tctattctcg tggtcatcga
     3001 cgcaaagtta gcaggcgcgt atcctcgcag atctacgctc acgatgcgac aatttaatcg
     3061 gttcttcatc gggtggtggg tcaggcatgg gttgcgggcg agggatcgga tcgggcactg
     3121 gaacaggatc gccaggaatc ggttcaggga caggaatttg caaataaata agtgtcgtca
     3181 tatttccctc cggtcattgg gtggactctt aaagggtaga cgctgataaa taacaggcaa
     3241 aaaaaagccg actcatcaaa gtcggcgtcg tacgaatcaa ttgtgctatg cagtaattca
     3301 aaaaaggaag taagacaata tggagcgcaa cgcccatcgc ttgacgttgc attcacctgc
     3361 aagggagata ttgccctgaa tgggtagaga gtttattgac ttcgctcaaa ctttgcggcg
     3421 tttttgtata cagacagccg gaaaaattgc ttttgttaca accatttact acggtgcaac
     3481 cataaagcaa caccaccaat aagaacaact aacagaatac aaaaaattga aaatccgaat
     3541 tgccacccgc cgccagggat cccaccaagg ttgacgccaa ataacccggt cagaaaggta
     3601 ctgggtaaaa agaccattgc catcaacgac attgtatagg tacgacgagc taaattttcc
     3661 tgcatcacct gagcgatttc atccgccatc acgccagtcc gtgctataca ggcgtcgatt
     3721 tcgtcaaggc cgcgcccaag gcgatcggca atatcctgca tccgacggcg ttggtcatcg
     3781 ctcatccacg gcaaacgttc actggcaaga cgagcataaa catcacgttg cggtgccata
     3841 tagcgacgca tcacaattaa ttgtttgcgc agcagagcca ggaatccacg cggtggaatt
     3901 tgctgatcaa ggagattatc ttcaaggtcg ataattttat cgtgcagctg ctcgataaat
     3961 tcgctggaat gatcggtcaa cgcatcgcac acatccacca gccaccctcc gcaatcggtc
     4021 ggacccgtgc cctcttccag atcgctcacc acatcgtcca gcgccagcac tttgcgttgt
     4081 cgggtcgaaa caattaaccg cccgtccata tatacacgca tggcgaccag ttgatcgggg
     4141 cgttcatcgg tgctgccgtt tatacagcgc aatgtaatca gcgtgccttc accgagacgg
     4201 ctgactcggg gccgcgtgct ctcgcccgcc agcgcatcac gtacgttatt gggaagcagc
     4261 ggtgttgtcg ccagccattg ggcgctatca tggtgtacat aattaaggtg gagccagcag
     4321 ggatgcgctt catcaatcac atctgtattt tccagcggtt taacgccgcc tctaccatcc
     4381 agcatccagg caaatactgc atccgggaca ttaacgtccg atcccttaat cgcttccaca
     4441 gtgcctccat catcaacgca ttattttgta gtctagcctt ctggccctgt tacgcaacat
     4501 ctcatcaccc cattaccctg aaatgattaa taaaattctg tctaaattga atacaaaaag
     4561 caaaatgctt ttccgtatac aaaccgtgtg aagtgttaaa tagcgtctat cattatcaga
     4621 attatctgat catatgacgt ggcttttttg cgatcggata gcaacaaaaa ttgataaaaa
     4681 taacgggatc tcaatgatta cgcacaactt caataccctg gacttactca ccagtcctgt
     4741 ctggatcgtt tcgccctttg aggaacagtt aatttatgcc aatagcgcgg cgcgactgtt
     4801 gatgcaagac ctcacgttta gtcagctacg aaccggtccc tattccgtct cctcacaaaa
     4861 agaactgccg aaatacctct ccgatctgca aaaccaacac gatattatcg aaatcctcac
     4921 tgttcagcgt aaagaagagg aaacagcatt aaactgtcgg cttgttttgc gaaagctgac
     4981 agaagcagaa ccggtgatta ttttcgaagg tatcgaagcg tcggcaacgc tgggtttaaa
     5041 agccagtcgc tcggcaaatt atcagcgcaa aaaacaaggt tttta
//
LOCUS       DAZPDU010000246         4987 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZPDU010000246 DAZPDU010000000
VERSION     DAZPDU010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4987
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.250"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            127..1101
                     /gene="hemB"
                     /locus_tag="ACHURS_004178"
     CDS             127..1101
                     /gene="hemB"
                     /locus_tag="ACHURS_004178"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFR1239773.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1207..2058)
                     /gene="tauD"
                     /locus_tag="ACHURS_004179"
     CDS             complement(1207..2058)
                     /gene="tauD"
                     /locus_tag="ACHURS_004179"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFR1239774.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRYQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(2055..2882)
                     /gene="tauC"
                     /locus_tag="ACHURS_004180"
     CDS             complement(2055..2882)
                     /gene="tauC"
                     /locus_tag="ACHURS_004180"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFR1239775.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     VLAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(2879..3646)
                     /gene="tauB"
                     /locus_tag="ACHURS_004181"
     CDS             complement(2879..3646)
                     /gene="tauB"
                     /locus_tag="ACHURS_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFR1239776.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3659..4621)
                     /gene="tauA"
                     /locus_tag="ACHURS_004182"
     CDS             complement(3659..4621)
                     /gene="tauA"
                     /locus_tag="ACHURS_004182"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR1239777.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         1171 a         1428 c         1245 g         1143 t
ORIGIN      
        1 tattacaaag atgtcctggt ctctctttca ttctcttctt gtcaacatcg cgacaacttt
       61 cgtaaaacac tcctgccccg cttcaggtat actgtgcacc ttaattccac aaacatcagg
      121 cagaccatga cagacttaat ccaacgccct cgtcgcctac gcaaatctcc tgcgctgcgc
      181 gctatgtttg aagagacaac acttagcctt aacgacctgg tgttgccgat ctttgttgaa
      241 gaagaaattg acgactacaa agccgttgaa gccatgccag gcgtgatgcg cattccagag
      301 aaacatctgg cacgcgaaat tgaacgcatc gccaacgccg gtatccgttc cgtaatgact
      361 tttggcatct ctcaccatac cgatgaaacc ggcagcgacg cctggcggga agatggactg
      421 gtggcgcgta tgtcgcgcat ctgcaagcag actgtgccag aaatgatcgt catgtcagac
      481 acttgtttct gtgaatacac ttctcacggt cactgcggtg tgctgtgcga gcatggcgtc
      541 gacaacgacg cgactctgga aaatttaggc aagcaagccg tggttgcagc tgctgcaggc
      601 gcagacttca tcgccccttc cgccgcgatg gacggccagg tacaggcgat tcgtcaggcg
      661 ctggacgctg caggctttaa agatacggcg attatgtcgt attcgaccaa gttcgcctct
      721 tccttttatg gcccgttccg tgaagctgcc ggaagcgcat taaaaggcga ccgcaaaagc
      781 tatcagatga acccaatgaa ccgtcgcgag gcgattcgtg aatcactgct ggatgaagcc
      841 cagggcgcag actgcctgat ggttaaacct gctggagcgt acctcgacat cgtgcgtgag
      901 ctgcgtgaac gtactgaatt gccgattggc gcgtatcagg tgagcggtga gtacgcgatg
      961 attaagttcg ccgcgctggc gggtgctata gatgaagaga aagtcgtgct cgaaagctta
     1021 ggttcaatta agcgtgcggg tgcggatctg attttcagct actttgcgct ggatttggct
     1081 gagaagaaga ttctgcgtta atttttttcc tctcagaaag ggggagttaa cctgttgaaa
     1141 gtacggtgaa cgccccctca ccctaaccct ctccccaagg gggcgaggga accgtccgca
     1201 ctcgtatcac cccgcccgat aaaacggttt atccccaagg atcgtcgccc gatgcataat
     1261 ccgccgctgt ggcaggtaat cggcattggc atagtgctga gtcacgcggt tatcccaaat
     1321 cgcaatatca ttcggttgcc agcgccaacg cacctgaaac tccggtttag tgatatgggc
     1381 gaataaaaag cctaacaagg cttcgctctc tttctcgctg acatcgacaa ttcgcgtagt
     1441 aaagccttca ttcacaaaca gcgcctgttt accgctcacc ggatgcgttc gcaccaccgg
     1501 atgtagcaac ggaggatttt tcgcgaccgc ctcgcgccag cgttgatgtt cctcctcggt
     1561 tttgcggtat ttgtattccg ggaacgattt acggaaatca tgctccgcac gcagcccgct
     1621 cagcagctgg cggaagggaa cagagagcgc ctcataggcc gcaataccgc tggtccagag
     1681 cgtatcaccg ccggtcgaag gtaactcttt agctgccaga atcgcccctg cgggtggcgt
     1741 ttcaataaat gtcacatcgg tatgccagtt gtcgttatcc ggcggattat cgttatgggt
     1801 atccagcacg atgatctcgt caaccccttc ggcatgaggg taaacagggt gaatatgcag
     1861 ttcgccaaaa cgctgggcca gcgcgcgttg ctgctgcggc gtaatagctt gatcgcgcag
     1921 aaataccacc tgatagcgca gcaccgcatg gtaaagctgt tcaaactgat tatcgcttaa
     1981 cgggcgcgtc aggtcggcac ccgaaatttg tgcgccgata tacggcccca gcggggtaat
     2041 actcagacgt tcactcattg tacttctcca tgccagggcg tcagacggcg ctgtaacgcg
     2101 cgcagaccca gttctaaaag aaaggcgata atcgcaatca ccgcgatccc cgccagtacc
     2161 acgtcagtcg cgagaaattc acccgctgac tgaaccataa atcctaaacc gcgtgtcgcg
     2221 gcaatcagct ccgccgctac cagcgtagac cagcccacac ccagaccaat gcgtaatccg
     2281 gtgaggattt ccggcagcgc gccgggcaaa atgacaaacc acagcacctg cgcacggctg
     2341 gcacccagcg actgggcggc acgaatgcga acctgctgcg cgcttttcac ccccgccagc
     2401 accgacatcg ccaccggtgc aaaaatcgct aaatagatca ataagatctt cgaggtttca
     2461 ccaataccaa accagatcac catcagcggc aaataagcca gcggcggcac cggacgataa
     2521 agttcgatta ccggatccag aatgccgcgc accgtcgggc ttagccccat ggcgatcccg
     2581 accggaatac cgatcaccac cgccgcgagt agcgccagca caatgcgcgt caggctggct
     2641 gccagatgct gccacagcgt ggcgtccata aatccctgcg gtccggcaat ggtgagcagt
     2701 tttgccagta cttgttgcgg cggcggcaaa aacagcgggc taatcagttg cagcgccgcc
     2761 accgtccacc atacagtgag taaaaccgct aacgtgccaa tgcttaaggt cacctgacgc
     2821 gagagcggcc agcgccattt cagccgccgc gaatgcagtt tttcattaat gagcacactc
     2881 atgagaacgc ctcccgttgc tcaaatacgc ggcttaaaac atattcgcgc atggcgatga
     2941 attgtggatc ggacttgatg ctgcggctcg actctcccgc aacaaagcgg cgagcaaagt
     3001 tgagcggcag ccgctccagc acacggccag ggccggatga aagcagaacc agttcagtcg
     3061 ccataaacac cgcttcttct atatcgtggg taatcaacag cacctgcttg cccgtctcct
     3121 gccagagttt cagcagcaga gtttgcatct ggtcgcgggt gaaggcgtcc agcgcaccaa
     3181 acggttcgtc gagtaataac agctggggat tcgccgccag cgcgcgggca atccccaccc
     3241 gctgacgttg accaccggaa agctgccaga tatagcgttt ttctgcgcct tccagcccca
     3301 cttttttcag catctggtgc gcgatttcca gtcgctgcat tttctctata cctgccaatt
     3361 gcaggccgaa cgccacgttg tcctgtacat tgcgccacgg tagtagccct tcattctgaa
     3421 aaaccacccc gcgctctgct cccggtccct caatacgctt acccgccagt tgaatgctgc
     3481 catgctgata aggcacaaaa ccggcaatca gattcagcag ggtggtttta ccgcagccgg
     3541 acggccccag caccaccagt agctcgccgc tttccagcgt caggttgata tcttccagtg
     3601 ccggtttgcc gccataatcg gcatagagat gagagatttg cagcatctgc gcctcctttt
     3661 attgcacgaa gcgcgaggta acgtactggc tgtaatcatt cgctacagcc gggaccttgc
     3721 cctgctcttt caaaaactgc gcggtgtcga tgattgcttt gttcaccggt ccggtcagtt
     3781 ctgccgtttg ttgctgcggc gtcagatagg tattcccctt caccagcccc ggaacgtcac
     3841 cttccggcac gccgcttaaa cgcgccagtt tgctgatgtt ttccggctgt ttcagccacg
     3901 cgtctgggtt agcaatgtac ggttgctgag catcgatggc gcttttagcg aacgctttca
     3961 cgacctcagg atgtttctcg gcaaaatctt tgcgcaccac ccagacgtcc agcgttggcg
     4021 caccccactg cccgacctgt tcagaatcgg tcaacacctt gccgtctttt tccagggcgt
     4081 taaccgccgg tgcccagaca taagcaccat caatatcacc ccgctgccat gcggcgataa
     4141 tcgcgggcgg ctgcaggttc acaatctcca cttgccctgg tttaatcccc cagtgtttca
     4201 gcgccgccag caggctgtag tgggtggtgg agataaacgg tacggcaatg cgtttgccaa
     4261 tcaaatcttc gggtttgctg atagttttct ttaccaccag cgcttcggag ttacccagtt
     4321 ttgacgccag caagaagact tcaatcggca cctgttggct ggctgcaacc gctaatgggc
     4381 tggaaccgag gttgccgatt tgcacgtcgc cagaagccag cgcccgcacg atgctggctc
     4441 cgctgtcaaa cttacgccag tccacgctcg ccccgctctc tttagcaaag gtgttgtcgg
     4501 cctgagccac tttcgccggt tcggctgagg tttgatacgc cacggtgacg ttcaccgcct
     4561 gtgcctgaaa agcgatgaat gccagtgcgg caagaagtgt gttacgcgat gaaattgcca
     4621 tgattgtctg ctcccctgtc ttgttatggg agcagtattc aggaataaaa acattcatta
     4681 aaagaattag tcgttatcgc acagatgatt ttattcttag caaaaaaacg gcaaaggaaa
     4741 gattaaatat gcaattactc cgttctaaca attgggaaaa ttactacact tcattttagt
     4801 gatgcataaa caaccacatt acgagattta ttacttcatt gtttctatca atacatataa
     4861 taaaactatt atattcgcat tgtatatatc actgtatcat tctcaatcac tcgcatatta
     4921 cattcacata atgtatataa acaatacttg ttcacagaac atatttttta aatgcaatta
     4981 attgaca
//
LOCUS       DAZPDU010000247         4965 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZPDU010000247 DAZPDU010000000
VERSION     DAZPDU010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4965
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.251"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            259..1203
                     /gene="leuO"
                     /locus_tag="ACHURS_004183"
     CDS             259..1203
                     /gene="leuO"
                     /locus_tag="ACHURS_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFR1239778.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            complement(1358..1402)
                     /locus_tag="ACHURS_004184"
     CDS             complement(1358..1402)
                     /locus_tag="ACHURS_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239779.1"
                     /translation="MKRNDKILHYEGLN"
     gene            1521..3245
                     /gene="ilvI"
                     /locus_tag="ACHURS_004185"
     CDS             1521..3245
                     /gene="ilvI"
                     /locus_tag="ACHURS_004185"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFR1239780.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVHNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3248..3739
                     /gene="ilvN"
                     /locus_tag="ACHURS_004186"
     CDS             3248..3739
                     /gene="ilvN"
                     /locus_tag="ACHURS_004186"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFR1239781.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVASTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIREVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            3751..3837
                     /locus_tag="ACHURS_004187"
     CDS             3751..3837
                     /locus_tag="ACHURS_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFR1239782.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            3919..4923
                     /gene="cra"
                     /locus_tag="ACHURS_004188"
     CDS             3919..4923
                     /gene="cra"
                     /locus_tag="ACHURS_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFR1239783.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREVAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
BASE COUNT         1207 a         1122 c         1310 g         1326 t
ORIGIN      
        1 tgtagatgat tgagtattcg cggtagttat gattagattg ttttcgcaac aaaaacatta
       61 tggattctta tgctgtggta aatgactcat tccacggcaa tggattctgt ttttatcaga
      121 acccgtatct ttatgttttc cgaattttac tcattttgct ttttcttatt ttatatgcat
      181 gataaatcat attcttcagg attatttctc tgcattccaa taagggaaag ggagttaagt
      241 gtgacagtgg agttaagtat gccagaggta caaacagatc attcagagac ggcggagtta
      301 agcaagccac agctacgcat ggtcgatctc aacttattaa ccgttttcga tgccgtgatg
      361 caggagcaaa acattacccg tgccgctcat gttctgggta tgtcgcaacc tgcggtcagt
      421 aacgctgttg cacgcctgaa ggtgatgttt aatgacgagc tttttgttcg ttatggccgt
      481 ggtattcaac cgactgctcg cgcatttcaa ctttttggct cagttcgtca ggcattgcaa
      541 ctagtacaaa atgaattgcc tggttcaggt tttgaacccg cgagcagtga acgtgtattt
      601 catctttgtg tttgcagccc gttagacagc attctgacct cgcagattta taatcacatt
      661 gagcagattg cgccaaatat acatgttatg ttcaagtctt cattaaatca gaacactgaa
      721 catcagctgc gttatcagga aacggagttt gtgattagtt atgaagactt ccatcgtcct
      781 gaatttacca gcgtgccatt atttaaagat gaaatggtgc tggtagccag caaaaatcat
      841 ccaacaatta agggcccgtt actgaaacat gatgtttata acgaacaaca tgcggcggtt
      901 tcgctcgatc gtttcgcgtc atttagtcaa ccttggtatg acacggtaga taagcaagcc
      961 agtatcgcgt atcagggcat ggcaatgatg agcgtactta gcgtggtgtc gcaaacgcat
     1021 ttggtcgcta ttgcgccgcg ttggctggct gaagagttcg ctgaatcctt agaattacag
     1081 gtattaccgc tgccgttaaa acaaaatagc agaacctgtt atctctcctg gcatgaagct
     1141 gccgggcgcg ataaaggcca tcagtggatg gaagagcaat tagtctcaat ttgcaaacgc
     1201 taactgaatg cagaataggt cagacatgaa tgtctggttt attctgtatt ttttattgaa
     1261 tgtagaattt tattctgaat gtgtgggctc tctattttag gattaattaa aaaaatagag
     1321 aaattgctgt aagttgtggg attcagccga tttattatca atttaatcct tcgtaatgga
     1381 ggattttatc gtttcttttc acctttcctc ctgtttattc ttattaccct gcgtttatgt
     1441 ctctggctgc caattgctta agcaagatcg gacggttaat gtgttttaca cattttttcc
     1501 gtcaaacagt gaggcaggcc atggagatgt tgtctggagc cgagatggtc gtccgatcgc
     1561 ttatcgatca gggcgttaaa caagtattcg gttatcccgg aggcgcagtc cttgatattt
     1621 acgatgcatt gcataccgtg ggtggtatcg atcatgtttt agttcgtcat gagcaggcgg
     1681 cagtgcatat ggccgatggt ctggcgcgcg cgaccgggga agtcggcgtc gtgctggtaa
     1741 cgtcgggtcc aggggcgacc aatgcgatta ctggcatcgc caccgcttat atggattcca
     1801 ttccattagt tgtcctttcc gggcaggtag cgacctcgtt gataggttac gatgcctttc
     1861 aggagtgcga catggtgggg atttcgcgac cagtggttaa acacagtttt ctggttaagc
     1921 aaacggaaga cattccgcag gtgctgaaaa aggctttctg gctggcggca agtggtcgtc
     1981 ctggaccagt agtcgttgat ttaccgaaag atattcttaa tccggcgaac aaattaccct
     2041 atgtctggcc ggagtcggtc agtatgcgtt cttacaatcc cactactacc ggacataaag
     2101 ggcaaattaa gcgcgctctg caaacgctgg tagcggcaaa aaaaccggtt gtctacgtag
     2161 gcggtggggc aatcacggcg ggctgccatc agcagttgaa agaaacggtg gaggcgttga
     2221 atctgcccgt tgtttcctca ttgatggggc tgggagcgtt tccggcaacg catcgtcagg
     2281 cactgggcat gctgggaatg cacggtacct acgaagccaa tatgacgatg cataacgcgg
     2341 atgtgatttt cgccgtcggg gtacgatttg atgaccgaac gacgaacaat ctggcaaagt
     2401 actgcccaaa tgccactgtt ctgcatatcg atattgatcc tacttccatt tctaaaaccg
     2461 tgactgcgga tatcccgatt gtgggggatg ctcgccaggt cctcgaacaa atgcttgaac
     2521 tcttgtcgca agaatccgcc catcaaccac tggatgagat ccgcgactgg tggcagcaaa
     2581 ttgaacagtg gcgcgctcgt cagtgcctga aatatgacac tcacagtgaa aagattaaac
     2641 cgcaggcggt gatcgagact ctttggcggt tgacgaaggg agacgcttac gtgacgtccg
     2701 atgtcgggca gcaccagatg tttgctgcac tttattatcc attcgacaaa ccgcgtcgct
     2761 ggatcaattc cggtggcctc ggcacgatgg gttttggttt acctgcggca ctgggcgtca
     2821 aaatggcgtt gccagaagaa accgtggttt gcgtcactgg cgacggcagt attcagatga
     2881 acatccagga actgtctacc gcgttgcaat acgagttgcc cgtactggtg gtgaatctca
     2941 ataaccgcta tctggggatg gtgaagcagt ggcaggacat gatctattcc ggccgtcatt
     3001 cacaatctta tatgcaatcg ctacccgatt tcgtccgtct ggcggaagcc tatggtcacg
     3061 tcgggatcca gatttctcat ccgcatgagc tggaaagcaa acttagcgag gcgctggaac
     3121 aggtgcacaa taatcgcctg gtgtttgttg atgttaccgt cgatggcagc gagcacgtct
     3181 acccgatgca gattcgcggg ggcggaatgg atgaaatgtg gttaagcaaa acggagagaa
     3241 cctgattatg cgccggatat tatcagtctt actcgaaaat gaatcaggcg cgttatcccg
     3301 cgtgattggc cttttttccc agcgtggcta caacattgaa agcctgaccg ttgcgtcaac
     3361 cgatgatccg acattatcgc gtatgaccat ccagaccgtg ggcgatgaaa aagtacttga
     3421 gcagatcgaa aagcaattac acaagctggt cgatgtcttg cgcgtgagtg agttggggca
     3481 gggcgcgcat gttgagcggg aaatcatgct ggtgaaaatt caggccagcg gttacgggcg
     3541 tgacgaagtg aaacgtaata cggaaatatt ccgtgggcaa attatcgatg tcacaccctc
     3601 gctttatacc gttcaattag caggcaccag cgataagctt gatgcatttt tagcatcgat
     3661 tcgcgaggtg gcgaaaattg tggaagttgc tcgttctggt gtggtcggac tttcgcgcgg
     3721 cgataaaata atgcgttgag aatgatctga atgcgcaatt tgcagcccaa catgtcacgt
     3781 tgggcttttt ttgcgaaatc agtgggaacc tggaataaaa gcagttgccg cagttaattt
     3841 tatgcgctta gatgttaatg aatttaaccc ataccagtac aatggctatg gtttttacat
     3901 tttacgcaag gggcaattgt gaaactggat gaaatcgctc ggctggcggg agtgtcgcgg
     3961 accactgcaa gctatgttat taacggcaaa gcgaagcaat accgtgtgag cgacaaaacc
     4021 gttgaaaaag tcatggctgt ggtgcgtgag cacaattacc acccgaacgc cgtggcagct
     4081 gggcttcgtg ctggacgcac acgttctatt ggtcttgtga tccccgatct ggagaacacc
     4141 agctataccc gcatcgctaa ctatcttgaa cgccaggcgc ggcaacgggg ttatcaactg
     4201 ctaattgcct gctcagaaga tcagccagac aacgaaatgc gctgtattga gcacctttta
     4261 cagcgtcagg tcgatgccat tattgtttcg acgtcgttgc ctcctgagca tcctttttat
     4321 caacgctggg ctaacgaccc gttcccgatt gtcgcgctgg accgcgccct cgatcgtgaa
     4381 cacttcacca gcgtggttgg tgccgatcag gatgatgccg aaatgctggc ggaagagtta
     4441 cgtaagtttc ccgccgagac ggtgctttat cttggcgcgc tgccggagct ttctgtcagc
     4501 ttcctgcgtg aacaaggttt ccgtactgcc tggaaagatg atccgcgcga agtgcatttc
     4561 ctgtatgcca acagctatga gcgggaggtg gctgcccagt tattcgaaaa atggctggaa
     4621 acgcatccga tgccgcaggc gctgttcaca acgtcgtttg cgttgttgca aggggtgatg
     4681 gatgtcacgc tacgtcgcga tggtaaactg ccttctgacc tggcaattgc cacctttggc
     4741 gataacgaac tgctcgactt cttacagtgc ccggtgctgg cagtggctca acgtcaccgc
     4801 gatgtcgcag agcgtgtgct ggagattgtc ctggcaagcc tggacgaacc gcgtaagcca
     4861 aaacctggtt taacgcgcat taaacgtaat ctctatcgcc gcggtgtgct cagccgtagc
     4921 taagccgcga acaaaaatac gcgccaggtg aatttccctc tggcg
//
LOCUS       DAZPDU010000248         4957 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZPDU010000248 DAZPDU010000000
VERSION     DAZPDU010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4957
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.252"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            17..568
                     /locus_tag="ACHURS_004189"
     CDS             17..568
                     /locus_tag="ACHURS_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFR1239784.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARM
                     ESPGKDATPGNVYAQADYKIAYE"
     gene            628..3132
                     /locus_tag="ACHURS_004190"
     CDS             628..3132
                     /locus_tag="ACHURS_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR1239785.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLNALINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTL
                     LGFTDEIIEEAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQANNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPP
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQGQLDVKVEEE
                     DGQVTQWQVASNSIPYLTRKGQIRYTTAMGKPTSVGGDSLQQPFFWTGEFSWGWLNNV
                     SLYGGSVLTNRDYQSLAAGVGFNLNSLGSLSFDVTRSDAQLHNQDKETGYSYRANYSK
                     RFESTGSQLTFAGYRFSDKNFVTMNEYINDTNHYMNYQNEKESYIVTFNQYLESLRLN
                     TYVSLARNTYWDASSNVNYSLSLSRDFDIGPLKNVSASLTFSRINWEEDNQDQLYLNI
                     SIPWGTSRTLSYGMQRNQDNKISHTASWYDSSDRNNSWSVSASGDNDEFKDMKASLRA
                     SYQHNTENGRLYLSGTSQRDSYYSLNASWNGSFTATRHGAAFHDYSGSADSRFMIDAD
                     GAEDIPLNNKRAVTNRYGIGVIPSVSSYITTSLSVDTRNLPENVDIENSVITTTLTEG
                     AIGYAKLDTRKGYQIMGVIRLADGSHPPLGISVKDKTSHKELGLVADGGFVYLNGIQD
                     DSKLTLRWDDKSCFIQPPNSSNLTTGTVILPCISQN"
     gene            3148..3897
                     /locus_tag="ACHURS_004191"
     CDS             3148..3897
                     /locus_tag="ACHURS_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR1239786.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMSYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVG
                     YINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            3899..>4957
                     /locus_tag="ACHURS_004192"
     CDS             3899..>4957
                     /locus_tag="ACHURS_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239787.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEGGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITY"
BASE COUNT         1538 a         1012 c         1020 g         1387 t
ORIGIN      
        1 tatataaagg aatattatgt ctgcttttaa aaagtcactt cttgttgctg gcgtagcaat
       61 gattctttcc aataacgtat ttgctgacga aggtcacggc attgttaaat ttaaaggtga
      121 agttatctct gctccatgtt caattaaacc aggtgacgaa gacttaactg taaatctggg
      181 tgaagtagca gataccgttc tgaaaagcga ccagaaatct ttggctgagc ctttcactat
      241 tcacctgcaa gactgcatgt tgagccaggg tggaaccact tattcaaaag cgaaggttac
      301 tttcaccaca gcgaatacta tgacgggtca gactgacctg ctgaaaaaca ccaaagaaac
      361 tgaaatcggt ggtgccacag gcgttggcgt acgtattctg gatagccaga gcggcgaagt
      421 gactttaggt actccggtag taattacatt caacaatact aacagctatc aggaactgaa
      481 cttcaaagct cgtatggaat ccccgggcaa agatgctacc ccgggcaacg tttacgctca
      541 ggctgactat aagattgctt acgagtaatc acattaggcc gggttcgccc ggccacatca
      601 ttaacggatt aatgataagt ggaacagatg tataaaaaat taaaattaac cacaataagc
      661 gaattgatta aaaatattta ttgttcatta tccgttctca tcattggttg tgcgtcagct
      721 tatgccgttg aattcaacaa agatttaatc gaagccgaag atcgtgaaaa cgttaacctt
      781 tcccaatttg aaactgatgg ccaattaccc gtaggtaaat actcattaaa cgctcttatt
      841 aataataaaa ggacgccaat ccatctggac ctgcaatggg tattaattga taaccaaact
      901 gcagtttgtc tgactccaga gcaattaaca ttattaggct ttactgatga gattattgaa
      961 gaggctcagc aaaacctgat cgatggttgt taccctatcg aaaaagaaaa acaaattaca
     1021 acttatctcg ataaagggaa aatgcaatta tccatatctg cacctcaggc atggttaaaa
     1081 tacaaagatg caaactggac gcctcctgaa ctttgggatc atggtattgc tggggcattt
     1141 cttgactaca atttatatgc ctctcattat gcaccacatc agggcgataa ttcacaaaat
     1201 ataagttcct atgggcaggc tggggttaat cttggggcct ggcgcctgcg tactgattac
     1261 cagtacgatc agtcatttaa caatggcaaa agccaggcga acaacctgga ttttccgcgt
     1321 atttatttgt ttcgcccaat cccagcaatt aatgcaaaac taactatagg tcaatacgat
     1381 actgaatcct ctattttcga ctctttccat ttttctggcg tttcgttgaa aagcgatgag
     1441 aatatgttgc cgcctgatct acgtggttac gcaccgcaaa tcacgggtgt tgcacaaacg
     1501 aatgcaaagg tcactgtctc acagaacaac cgtattattt atcaagaaaa tgttcctcct
     1561 ggcccatttg ctattactaa tttattcaat acattacagg ggcaactgga cgtcaaggtt
     1621 gaagaagagg acgggcaagt cacgcaatgg caagttgcat ctaatagtat tccttatctg
     1681 acgcgtaaag ggcagattcg ctacaccact gctatgggta aaccgaccag cgttggtggt
     1741 gattccttac aacaaccctt cttctggact ggtgaattct catggggttg gctgaacaat
     1801 gtatccctgt atggtggttc agttttaaca aaccgtgatt atcaatctct ggctgccggc
     1861 gttggcttta accttaactc attgggttca ttatcttttg atgtcacacg atctgatgct
     1921 cagttgcata atcaggataa agaaacgggt tatagctacc gcgctaacta ttcaaaacgt
     1981 tttgaatcta ccggtagcca gctcactttc gctggttacc gtttctctga taaaaacttt
     2041 gtgacaatga atgaatatat caatgacact aaccattaca tgaattatca gaatgaaaaa
     2101 gagagttata ttgtcacgtt taaccagtat cttgaatcat taaggttaaa tacatacgta
     2161 agtttggctc gtaatactta ctgggacgcc agcagtaatg tgaattattc attatcactt
     2221 agccgcgatt ttgatatcgg gccattaaaa aacgtctccg cttctctaac atttagccga
     2281 ataaactggg aagaagacaa ccaggatcaa ctgtacctaa atatttcgat tccctgggga
     2341 accagtagaa cattgagcta tggtatgcaa cgaaatcagg ataacaagat ttcgcatact
     2401 gcttcgtggt atgactcttc cgatcgaaat aattcctgga gcgtttctgc ttcaggcgac
     2461 aatgatgaat tcaaagatat gaaagcgtca ctacgcgcca gttatcagca taataccgag
     2521 aacggtcgtc tctatctctc cgggacatca cagcgagaca gttattattc tctgaatgcc
     2581 agttggaatg gttcattcac ggcgactcgt cacggagccg ctttccatga ctatagtggt
     2641 agtgctgact cgcgttttat gatcgacgca gatggtgcag aagatattcc gttgaacaat
     2701 aaacgcgcgg taactaatcg gtatggcatc ggagttattc catcagtcag cagttacata
     2761 acaacatcat taagcgttga tacccgaaat cttccagaaa atgtggatat cgaaaactcg
     2821 gttatcacca ccaccttaac cgaaggtgct attggctacg ccaaacttga tacccgcaag
     2881 ggctatcaaa tcatgggggt tattcgcctg gcagatggta gtcacccacc actggggatt
     2941 agcgtaaaag ataaaaccag ccataaagaa ttaggactgg ttgctgatgg cggctttgta
     3001 tacctcaacg gcattcagga tgacagcaaa cttactttac gctgggatga taaatcctgt
     3061 tttattcaac cacccaatag cagcaactta accaccggaa cggttatttt accgtgtatt
     3121 agccaaaatt aattcaggat gaaactaatg cgctacttaa acacaaagaa tctcattgca
     3181 gcaggagtat tactcgcctg catgagcagc attgcctggg gtgccatcat cccggaccgt
     3241 acccgtatca taatgaatga gtcagataaa ggggaagcat taaaacttac taatcaaagc
     3301 aaaaatctac cctatctggc gcaaacatgg atagaagata cgaaaggcaa taaatcacgt
     3361 gactttattg tcacggttcc ccctatggta cgtttaaatc ccagtgagca aattcaaata
     3421 agaatgataa gtcaggaaaa aattgctcaa ctgcctaatg acagagaaac gctcttctat
     3481 tttaacgtgc gagaaatacc gccaaaaaca gataaaaaaa atgtgatgca agtcactatg
     3541 cagcacgcat tgaaactatt ctggcgacca aaagctattg aattagaaga tgatggggta
     3601 atgtcttacg aaaaagttga aatcatccgt agaaatgatg gttcgatccg ctttaacaat
     3661 aagatgcctt atcacgttac gctgggttat atcggaacaa acggaatcac aatgttgcca
     3721 caaacgcaaa gtttaatggt aaccccttat agccatgcag atacgcagtt taaaaatgtt
     3781 ccgtcagctt ttcaggttgg ttatatcaat gattttggtg gattaagttt ttatgaaata
     3841 aactgcccga cggttaataa ctcttgtaat gtttccgtag ccaaacgaga taaataatat
     3901 gcgctactta ttaattgtaa ttatatttat tataggtttt agtgcattac ctgtatgggc
     3961 aatggactgc tatgctgaac atgaaggggg aaataccgta gtcattggtt acgtaccaag
     4021 aatttctatc cccagtaatg gtaaaaaagg tgataagatc tggcaaagca gtgagtattt
     4081 tatgaatgtc ttctgtaata atgcattacc tgccccatca cctggagaag aatacccatc
     4141 agcctggact aatataatga tgtttttacc aggcggtcag gacttttata atcagaactc
     4201 ctatattttt ggcgtaacat acaatggagt tgattatgat tcgactgccc ctaatgctat
     4261 cgcagcacca gaatgcattg atataaaagg agcgggtacc tttaataatc actataaaaa
     4321 ccccgctgtc tgtagtggag gtcctgagcc tcaattatca gtaacctttc cagctcgcgt
     4381 acaactctat attaaattag caaaaaatgc caatagagtt aataaaaacc tggtgttacc
     4441 tgatgaatat atcgctctag agtttaaagg tatgagtggc gcaggagcta tagaagtaga
     4501 caaaaatttg accttcagga ttcgtggatt aaataatatt catgtccttg actgctttgt
     4561 taatgtcgca ctggaaccag ctgatggcgt tgttgacttt ggtaaaataa attcccgaac
     4621 aattaaaaat accagcgtta gtgagacgtt tagtgtagtc atgacaaaag atcctggtgc
     4681 ggcctgtact gagcaattca atattttagg gagctttttc actacggata ttttgagtga
     4741 ttatagccat ctggatatgg gtaatggact gctattgaag atatttcata aagatggaac
     4801 agcaacggaa tttaaccgct tctcacaatt tgcttctttt tcatcgtcta gtgcaccttc
     4861 ggtcaccgca ccattcaggg cagaacttag tgcgaacccg gcagagactg ttgttgaggg
     4921 accgtttagt aaagacgtaa tcctcaaaat cacctat
//
LOCUS       DAZPDU010000249         4890 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZPDU010000249 DAZPDU010000000
VERSION     DAZPDU010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4890
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.253"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            12..754
                     /locus_tag="ACHURS_004193"
                     /pseudo
     CDS             12..754
                     /locus_tag="ACHURS_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308314.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(796..1248)
                     /locus_tag="ACHURS_004194"
                     /pseudo
     CDS             complement(796..1248)
                     /locus_tag="ACHURS_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            1312..1458
                     /locus_tag="ACHURS_004195"
                     /pseudo
     CDS             1312..1458
                     /locus_tag="ACHURS_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1458..2615)
                     /gene="intS"
                     /locus_tag="ACHURS_004196"
     CDS             complement(1458..2615)
                     /gene="intS"
                     /locus_tag="ACHURS_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HFR1239788.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIVPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMKGYRKKNFPFLPADQMPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMLWKNVDFENRIITIDASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDEKVE"
     gene            complement(2777..2851)
                     /locus_tag="ACHURS_004197"
     tRNA            complement(2777..2851)
                     /locus_tag="ACHURS_004197"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2816..2818),aa:Arg,seq:cct)
     gene            complement(2927..3859)
                     /locus_tag="ACHURS_004198"
     CDS             complement(2927..3859)
                     /locus_tag="ACHURS_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFR1239789.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            4153..>4890
                     /gene="mlaA"
                     /locus_tag="ACHURS_004199"
     CDS             4153..>4890
                     /gene="mlaA"
                     /locus_tag="ACHURS_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFR1239790.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLK"
BASE COUNT         1170 a         1143 c         1151 g         1426 t
ORIGIN      
        1 gggtggatga tatggcaact acatgttcag ttatattgat tttggagtcc tttgatgttt
       61 atttcggaaa agagagtgtg tttctggaga gaggttcatc tgtacttgtc gactctagct
      121 ctagagattt tttcctgaca tatcctgaaa gagtgatagt ggcggatttt ggcgctgagt
      181 ttattagtcg ctatttgaaa gctaataact taagggatat ttctgattgt agggaatatc
      241 catcttattt aaaaataaac tttgctgact tcagtttaat taaaggatta attagttggg
      301 ctaatcactg tgctgaatac atagaaattt ttgatgagtc tattgctttt acatgtctct
      361 ctgcattttc ttctgaaaaa caatttggag tatttctgtt tggatgtttg aaaagcacag
      421 ggctaaagtt aaaacgatta ttcatacgga tttatctgca ccatggcgtc ttaaggatat
      481 atcatcaaga ttatatctca gcgaaagttt actaaagatg aaattgaaag aagagggggt
      541 atcattcagt aagatcatac ttgatgagag gatgcaaatg gctgaatatt tactcagcac
      601 tcgttgttat cctattagta aagtagctaa ggtctgtggt tatgccagtg tctcatactt
      661 tacttatgta tttagacgtt attttggtgt ttctccaagt caatactctc agaggagttc
      721 agaaagtaaa attcttactc accagggaat ctgatcattg ttcttgcccc cttatttcca
      781 gacagggggt gtatcttaag ttaacgttac ccgctgacgt cgatattctc gcggagagcg
      841 ataacccagc gcactgtgcg gatggttttc attgtaatgt tcgatcgcca ctgcaagatt
      901 atgcaatgcc gttcttacat tcggtttcgg catgaacgcg atgtagtctt ccttcatcgt
      961 tttcacgaac ctttctgcca ttccattact ctgaggactg ctgattgccg ttgtgcaggg
     1021 ctccagattc aactctctgg cgaactgccg tgtttcatgc gctctatacg ctgaaccgtt
     1081 atctgtcagc cactggatgg actgttccgg tactttatcg ccaaagcgtt tttctatcgc
     1141 tcctaacatg acatcctgca ctgtcgcttt atcgtaacct cccgtgcttg cggcccagtc
     1201 tatggcttca cgatcgcaac agtccagggc gaacgtaaca cgcagttttg aatcgccacg
     1261 ggtttaacag acacctcaga gtcatttaag atggcttaaa gagaggtgcc catgagcggt
     1321 aagcgttatc ccgaagagtt taaaactgaa gcagtcaaac aggttgttga tcgcggttat
     1381 tctgttgcca gcgttgcaac acgtctcgat atcactaccc acagccttta tgcctggata
     1441 aagaagtacg gtccggatta ctccactttt tcatcaagcc aatccgccca ccactgcatc
     1501 atttctctgc gcttatcgag atactgagca tggttgtaaa taccgcgcac agatccgccg
     1561 ttggcatgtg ccagctgcac ttcaatagcg tcagcaggcc attcgtgctc gttcataatc
     1621 gtgctgaatt catgcctgaa tccgtgaccg ctttccagac cttcatagcc gatttgtttg
     1681 atcacaagca ataccgcgtt ctcgcagatt ggcttcttct tatcgttgcg cccggcaaaa
     1741 acaaactctg agactggttt ggtgattgag cttagcgtag tgagaagttc aaccacctgg
     1801 tctgacatcg ggaccacatg aattttgcgt cccttcatca cactggcgtc gatggtgata
     1861 atcctgtttt caaaatcgac gttcttccat agcatggaac gaagctcttt tgttcttagg
     1921 gctgtgtagc gtaaaacttt ggtcgcaatg agcgatacga tacttcctga aaatgttgcc
     1981 agtgctttgt tgaatgcagg catctggtct gctggaagga acgggaagtt cttcttgcgg
     2041 tatcctttca tggcgtctgc aaggtcaggt gccgggttat atttagccct tccggtgaca
     2101 atagcgtaac gaaaaacctc gccgcatctt ctgcgggctt tgttggctcg ctccattgca
     2161 ccgcgatctt caaatctgcg gattacttcc agcagttgca tcggctcaat atcctgaatc
     2221 tcaagaccgc cgatgatggg taaaatgtcg tcatcaaaca ttttggcaag ttcagttgca
     2281 tagcctactg accagacttg cttcttgtgc tcgtaccatt ccttgtaaat cgcactaaat
     2341 gaattgttgt tagacgaagc ctttttcgcc tttaccggaa cgatgccaac cgagatgtct
     2401 ttcctcgcag tccatgcctt atcccttgct tcctgcaaag tcataagcgg atattttccg
     2461 acggtcagga ttttctcctt accgtcaatc ttgtagcgaa gctgccatac ctttttcccg
     2521 gatacaggaa cataaaggta caggccatta ccatcgagaa ggcggtatgg tttttctttc
     2581 ggctttgctg cttcaatctg cttaacggtg agcatgggta aaaatccggt gggtaaaatt
     2641 attttatcca ctttttaccc gtcatggagt gcggctgtca acgatctgac gcgaaccatg
     2701 acgaaccgtg aatatacgga aggcttgata ttcaggggat tttgcggact ggtacggatg
     2761 ggagcgaact gataaatggt gtcccctgca ggaatcgaac ctgcaattag cccttaggag
     2821 gggctcgtta tatccattta actaagagga caatgcggca tgagtatacc cgctaatgga
     2881 gtgcggggta agtacgctgc cgcttgattg cttaaaccct cgccatttat gccgggtttt
     2941 tatcattttt cttaatgttt tccgcacgtt ctgctttttg gcgtgcttct gctttacgct
     3001 tgttgctcat gtcgttacga atctgtgcat gactcattaa cgcgaagata aaggtgccgc
     3061 cgcagatgtt ccccgctaaa gtaggtagtg cgaagggcca gatgaaatcg ctccagtgca
     3121 gcgtaccgtt aaacaccaga tagaggattt caacagaacc gaccacgata tgggtggtgt
     3181 cacctagggc aataagccag gtcatcaata taatcaccac aatctttgcc gcacctgctg
     3241 caggaaacat ccataccata gtggcgatca gccagccgga aatgatcgcg ttggcaaaca
     3301 tctcgctggg ggtgttcttc atcacatcca tgccgatttt gacaaatgca tcgcgagttt
     3361 cttcattgaa gataggcata tattcaaatg cccacgcagc aataccagtc ccgagaatat
     3421 tacccagcag cacgactccc cataaccgca taagtaagcc aacgttgctc attgtcggtt
     3481 tttgcatgac gggtagtacc gcagtcacgg tgttttcggt aaataattgc tggcgggcca
     3541 ttatgacgat aataaaacca aaggtataac cgagattctc cagtaagaag ctgcccggca
     3601 caccttccag ttcgacatga aatatccctt ttgccagtaa cgaagcgccc atcgacagac
     3661 ccgccgcaat ggctgaccac agtagcgcca ttgcgtcgcg ttccagctct ttttcaccat
     3721 cctggcggat atgctcatga attgccatcg cccgggaggg gagtcggtct tcatctattt
     3781 ctattttttt gccgcgctct ttttcttcgc tctcaacttc aatttcgtcg ctgtgttgat
     3841 caattttgtc gttgtccatg gtctcttctc ttgaattagc acgtatagct aaagcgtagc
     3901 ggcttttttg ctcgcaactg gcgggagtta ctctgaaaat gtagaaaagg ctgcgtttgc
     3961 ctttttctgt ttctatagaa tcaagtagcc tacagggcgg cgattaccag gctatgatca
     4021 aatcagcaaa tcagggcgtc tggacatcag ttgacgtgct gttacaatcg cccacaccta
     4081 aacaggcgga tacggtatcg ttccgtcatg gatggcaaac tgcataagcc ataaaaaaac
     4141 agggagacat ttatgaagct tcgcctgtcg gcgcttgctc tgggaactac gcttctggtg
     4201 gggtgtgcga gttccggtac agatcagcaa gggcgttctg acccgttaga agggttcaac
     4261 cgcaccatgt acaacttcaa cttcaacgta ttagacccgt atattgttcg accggtcgct
     4321 gtcgcctggc gtgattatgt tccgcaaccg gcgcgtaatg gtttgagcaa ctttactggc
     4381 aaccttgaag aacctgcggt gatggttaac tacttcttgc agggcgaccc ttatcagggg
     4441 atggtccact ttacccgctt tttcctgaac accattttgg ggatgggcgg ctttattgat
     4501 gttgcaggga tggcgaaccc gaaactgcaa cggactgaac ctcaccgctt cggtagtacg
     4561 cttggtcatt atggcgtggg ttatgggcct tacgttcagt taccgttcta cggtagcttc
     4621 acgctgcgtg atgacggtgg tgatatggcg gatggtcttt acccggttct ttcctggctg
     4681 acctggccga tgtctgtggg taaatggacg cttgaaggga tcgaaacccg cgctcagctg
     4741 ctggattccg atggtctgct gcgtcagtcg tccgatcctt atattatggt gcgcgaagcg
     4801 tacttccagc gtcatgattt catcgctaat ggcggcgaac tcaaaccgca ggaaaatccg
     4861 aacgcacaag cgattcagga tgatttaaaa
//
LOCUS       DAZPDU010000250         4880 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZPDU010000250 DAZPDU010000000
VERSION     DAZPDU010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4880
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.254"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..677)
                     /locus_tag="ACHURS_004200"
     CDS             complement(<1..677)
                     /locus_tag="ACHURS_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFR1239791.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPAPLPFILSRTYSSYRTKTPAPMGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQ
                     ALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGR
                     TQTFHREA"
     gene            906..1514
                     /locus_tag="ACHURS_004201"
     CDS             906..1514
                     /locus_tag="ACHURS_004201"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFR1239792.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1612..3003
                     /gene="selA"
                     /locus_tag="ACHURS_004202"
     CDS             1612..3003
                     /gene="selA"
                     /locus_tag="ACHURS_004202"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFR1239793.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEIIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPMIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            3000..4844
                     /gene="selB"
                     /locus_tag="ACHURS_004203"
     CDS             3000..4844
                     /gene="selB"
                     /locus_tag="ACHURS_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFR1239794.1"
                     /translation="MIIATAGHVDHGKTTLLQVITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPSFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPGREHASQHSVRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
BASE COUNT         1075 a         1281 c         1443 g         1081 t
ORIGIN      
        1 gcttcgcggt ggaacgtctg tgtgcgcccg aagcggtcca ccagcccggt cagtacccgg
       61 tacggcggca gcggcgcagg cagcacctca tccgcttccg gcacccgctc acaccagccg
      121 agcagccacc acggcccctg cggactgttt gtcgccagat aacgatgcgg acttaagcgg
      181 agttcttccg gcagcgcctg ccagagtgcg gccagccggt gaccttcatc cagtttcgcc
      241 acgccgccgc gcaccagcca gagtgattcg ctgcggctgt aaccgtcctc accgggaaac
      301 aggtgctcaa aatacaggct tctgccgccg ttatcactga gtatcagtgt gttatcgcgc
      361 agctgtaagc ggatatccgc aggcattttc cagccggggc cgaggctccc catcggcgcg
      421 ggcgtttttg tccggtaact gctgtaggtg cgggagagga tgaacggcag cggggcgggc
      481 agggcgatgt cggtttcacc gggaaggacc tttgcaccga gcaggggatt gaccggatgg
      541 ccggacgtca ctccgccggg gcacaccgaa caggccacac cggtgggggc accgatgcgc
      601 accccggctg aaccctgaac aatgctaccg ccatactgcg tcatgtcgcc ctgacgcgct
      661 gccggttttc cgctcatctg ctcatccttg ttttatcggg ttatttgtct gcttccaggc
      721 aaaaccctac agatttataa gaaagcctta agtgagatat ccatcagata gaaacaatta
      781 ggaaaatatt tatatcacca tgataaatat gtgatttatt tggaagtaac aaaccatcaa
      841 cattttctgc cctttttccc atcaccaggc catactgata tttttcctcg cacaaaggac
      901 tcggcatgaa actcgtcggt agctacacca gcccgtttgt acgcaaactt tctattctgt
      961 tgttagaaaa gggcataact ttcgaattta ttaatgaact gccctataac gcggacaacg
     1021 gcgtggcgca atttaacccg ttaggaaaag tgccggtgct ggtgaccgaa gagggcgaat
     1081 gctggtttga ttcgccgatc atcgctgaat atattgagtt aatgaatgtc gctccggcga
     1141 tgttgccgcg cgatccgctg gagtcgttgc gggtgcgcaa aattgaggcg ctggcggatg
     1201 gcattatgga tgccgggctg gtatcggtgc gtgaacaggc gcgtccggcg gcgcagcagt
     1261 ctgaagatga attgttacgc cagcgggaga aaatcaaccg cagtctggat gtgctggaag
     1321 gatatctggt cgatggcaca ctcaaaaccg atacggtcaa tctggcgact atcgccattg
     1381 cctgtgctgt cggatatctc aatttccgcc gcgttgcgcc aggttggtgt gtcgaccgcc
     1441 cacacttagt caaactggtc gaaaacctgt ttagccgcga aagttttgct cgcaccgaac
     1501 cgccaaaggc ttgatgcgcg atatgtcctc ctgacccatc tcacgttaca atccgtggtt
     1561 atgttaaacg cccttctccg tgtgagaggg ccttgatcag ccaggtttcc tatgacaacc
     1621 gaaacgcgtt ccctctatag tcaacttccg gctattgatc gcttattgcg cgatagctcc
     1681 ttcctttctt tgcgtgatac ttatggtcac acccgcgtgg tggaattgtt gcgtcagatg
     1741 ctcgacgaag cgcgagaagt gattcgtgac agccagacgc ttcctgcgtg gtgtgaaaac
     1801 tgggcgcaag aagtcgatgc ccggttgacg aaagaggcgc agagcgcgct gcgtccggtg
     1861 atcaacctga cgggaaccgt gctgcatacc aaccttggac gagctttaca ggcggaagcc
     1921 gcggtggaag ccgttgcgca ggctatgcgt tcgccagtga ccctcgagta cgatctggac
     1981 gacgccggac gcggacatcg cgatcgggcg ctggcgcaac tgctgtgccg tattacgggg
     2041 gcggaagatg cctgtatcgt caataacaat gcggcggcgg tgttattgat gttggcggcc
     2101 actgccagcg ggaaagaggt ggtagtatct cgcggcgaat tggtggagat tggcggcgcg
     2161 tttcgtattc ccgatgttat gcgtcaggca ggctgcaccc tacacgaagt agggaccacc
     2221 aaccgcacgc acgcgaatga ttatcgtcag gcggtgaatg aaaataccgc actgttgatg
     2281 aaagtacata ccagtaacta cagcattcag gggttcacca aagcgataga tgaagcggaa
     2341 ctggtggcgc tcggcaaaga gctggatgtt cccgtagtga ctgatttagg cagtggctcg
     2401 ctggtcgatc ttagccagta cggtttgccg aaagagccaa tgccgcagga gttgattgcg
     2461 gcgggcgtca gtctggtgag cttctccggc gacaagttgt taggcgggcc gcaggcaggg
     2521 attatcgttg gtaaaaaaga gataatcgcc cgcctgcaaa gccacccgct gaagcgtgca
     2581 ttacgcgcgg ataaaatgac cctcgcggcg ctggaagcca cgttgcgtct ttatttacac
     2641 cctgaagctc tgagtgaaaa attaccgacc ctgcgcctgc ttacccgcag cgcagaggtc
     2701 attcaaatcc aggcacaacg tttacaggct ccccttgccg cacattacgg cgcggagttt
     2761 gcggtacagg ttatgccatg tctttcgcag attggcagtg gttcgctgcc ggttgatcgc
     2821 ctgccgagcg cggcattaac gtttacaccc catgatggac gcggtagcca ccttgagtca
     2881 ttagccgccc gctggcgtga attgccagtg ccgatgattg gtcgtattta tgacggacga
     2941 ttgtggctgg atttacgctg ccttgaagat gagcaacggt ttttggagat gttgttgaaa
     3001 tgattattgc gactgccgga cacgttgacc acggcaaaac aaccttattg caggtgatta
     3061 ctggcgtaaa tgctgaccgt ctgccggaag aaaaaaagcg cggcatgacc atcgatctcg
     3121 gctatgccta ctggccgcag ccggatggtc gcgtgcctag ttttatcgac gtacccggtc
     3181 atgaaaagtt tctttccaac atgctggcgg gcgttggtgg tatcgatcac gcgctgttgg
     3241 tggtggcgtg cgatgacggc gtgatggcgc agacccgtga gcatctggcg attttgcagc
     3301 tgaccggtaa cccgatgctg acagtggcgc tgaccaaagc cgatcgcgtg gacgaagcgc
     3361 gtgttgatga ggttgaacgc caggtaaagg aggtgctgcg ggaatacggt tttgctgagg
     3421 caaaactgtt tatcaccgca gcaaccgaag gtcggggaat tgatgccctg cgcgagcatc
     3481 tgcttcagtt accaggacgt gaacacgcca gccaacatag tgtccgcctc gcgatcgacc
     3541 gcgcatttac cgtaaaaggt gccgggctgg tcgtcaccgg tacggcgtta agcggggaag
     3601 tgaaggtagg cgattcactc tggctgactg gtgtaaataa accgatgcgt gtacgtgcgc
     3661 tgcatgcaca aaaccagcca acagaaaccg cccatgccgg gcagcgtatc gcgcttaaca
     3721 tcgcgggtga tgcggaaaaa gagcagatta accgtggcga ctggctgctt gccgatgcgc
     3781 cgccagagcc gttcacacgg gtgattgtcg agcttcaaac ccatacgccg ctgacccagt
     3841 ggcagccgct gcatattcac cacgccgcca gccacgttac gggacgcgtt tcgctgctgg
     3901 aagataacct tgccgagctg gtgttcgaca cgccgctgtg gctggcggat aacgatcgct
     3961 tagtgctgcg cgatatctcg gcccgcaaca cgctggcggg ggcgcgtgtg gtgatgctta
     4021 acccgccgcg tcgcggtaaa cgtaagccgg aatatctgca atggctggcg tctcttgcac
     4081 gggcgcagag cgatgccgat gcgttatctg ttcatctgga acgcggcgcg gttaaccttg
     4141 cggatttcgc ctgggcgcgc cagctcaacg gcgaagggat gcgcgaattg ctgcaacagc
     4201 ctggctatat tcaggctggt tatagcttgt tgaatgcgcc ggttgccgcc cgctggcagc
     4261 ggaaaattct cgacacatta gcgacttatc atgagcaaca tcgcgatgaa cctggtcctg
     4321 ggcgcgaacg tctgcgacgt atggcgttgc caatggaaga tgaagcgctg gtactgttgc
     4381 tgattgaaaa gatgcgcgaa agcggcgaca tcctcagtca tcacggttgg ctgcatctgc
     4441 cggatcacaa agcgggcttc agcgaagagc agcaggccat ctggcaaaaa gcagagccgc
     4501 tgtttggtga cgagccgtgg tgggtgcgtg atctggcaaa agagacgggt accgacgagc
     4561 aggcaatgcg cctgacgcta cgccaggcgg cgcagcaagg aataattacc gcgatcgtca
     4621 aagatcgtta ttaccgtaac gatcggattg tcgaatttgc caatatgatc cgcgatctcg
     4681 atcaggagtg tggttcaacc tgcgcggcgg atttccgcga tcgcttaggc gtaggccgaa
     4741 agctggcaat tcagattctg gaatattttg accgcattgg ctttacgcgt cgtcgtggaa
     4801 atgatcattt attgcgcgac gcattattat ttccggaaaa ataaggaaat gattaattaa
     4861 tttttaaaat aaattaatac
//
LOCUS       DAZPDU010000251         4850 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZPDU010000251 DAZPDU010000000
VERSION     DAZPDU010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4850
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.255"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(91..2160)
                     /gene="glyS"
                     /locus_tag="ACHURS_004204"
     CDS             complement(91..2160)
                     /gene="glyS"
                     /locus_tag="ACHURS_004204"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFR1239795.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2170..3081)
                     /gene="glyQ"
                     /locus_tag="ACHURS_004205"
     CDS             complement(2170..3081)
                     /gene="glyQ"
                     /locus_tag="ACHURS_004205"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFR1239796.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3176..3475)
                     /locus_tag="ACHURS_004206"
     CDS             complement(3176..3475)
                     /locus_tag="ACHURS_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFR1239797.1"
                     /translation="MMMNAFFPAMALMVLVGWSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3650..4645
                     /gene="wecH"
                     /locus_tag="ACHURS_004207"
     CDS             3650..4645
                     /gene="wecH"
                     /locus_tag="ACHURS_004207"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFR1239798.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMAVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4687..>4850)
                     /locus_tag="ACHURS_004208"
     CDS             complement(4687..>4850)
                     /locus_tag="ACHURS_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YiaA/YiaB family inner membrane protein"
                     /protein_id="HFR1239799.1"
                     /translation="GLWNATLLLSEKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKET
                     QVTQEEYSE"
BASE COUNT         1112 a         1269 c         1229 g         1240 t
ORIGIN      
        1 tattcaacag aagattatta aaggttgctg tattatttag cgataataaa aaaagcctgc
       61 cagatggcag gctttttaat aacggcgtta ttactgcaac agcgaaatat ccgcaacgcg
      121 caggaacagt tcgcgcagtt tctccagcat ggtcagacgg ttgagacgca attctttgtc
      181 atcaaccatg accatcactt tatcgaagaa agcatcaacc ggctcacgca gctctgccag
      241 ttcgaccagc gcatcctggt aacgaccttc cgcaaagtac ggctccagct tgtcacgtag
      301 caccacaacc tgcatcgcca gtttaatttc ttccggctct ttcagggtag aggcattcac
      361 gcggtcgctc agcacttcgt cagatttcgc cagaatgtta gatacacgtt tgttcgccgc
      421 cgccagtgca gcagctgcat ccagggtacg gaaatgcgat accgctttca tacgggcatc
      481 gaaatcagcc ggacgagtcg gacgacgcgc cagtaccgcc tggatggtgt caacggtgta
      541 accttcgtcc tgataccagg cgcggaagcg accgagcata aagtcgataa catcatcaac
      601 tacgttggcg ttagtcagct tatcgccata cagacgcact gcttcttcgg tcagcgtttg
      661 cagatcaagg ttgaggttct tctcaacgat aattcgcagc acgccaagtg cggcacgacg
      721 cagcgcaaac gggtctttgt cgcctttcgg atgctgaccg ataccgaaga tacccgccag
      781 agtatccatc ttatcagcaa tcgccagcgc acaagctacc gggttggacg gcaggtcatc
      841 accggcaaag cgcggctgat actgctcatt cagcgcgacg gctacgtctt ccgcttcgcc
      901 atcgtgacgc gcgtagtgca tccccataac gccttgggtg tcggtgaact cgaagaccat
      961 gttggtcatc aggtcacact tagacagcag gcccgcacgg gttgcgtggt taacgtcagc
     1021 gccaatctgt tcagcaatcc agccagccag cgcctggatg cggtcagttt tgtcacgcag
     1081 cgtacccagc tgttgctgga acagcacggt ttgcaggcgc ggcaggttat cttccagacg
     1141 ttttttacgg tcggtattga agaagaactc ggcatccgcc agacgtggac gaacgacttt
     1201 ctcgttacca gagataattt gctgcggatc tttcgattcg atgttggcaa cgaagatgaa
     1261 gttcggcagc agtttgccat cgttcgcata caccgggaag tatttctggt cacctttcat
     1321 ggtgtaaacc agggcttcag ccggcaccgc gaggaatttc tcttcgaatt ttgcggtcag
     1381 aacgaccggc cactccacca gcgaagccac ttcttccagc aggctttcgc ttaagtcagc
     1441 gttaccgcca atcttacgcg ctgcttcttc ggcatcggct ttaatcttcg ctttacgttc
     1501 ttcgtaatcg gcgatgactt tcccgcgctc acgcagaatt tccggatact gatcggcgtt
     1561 gtcgatggtg aattccggtt cgcccataaa gcggtggccg cgaatcacgc gatcggactg
     1621 aatgcccaga atggttgccg gaatgacttt gtcgcccagc agcagggtca cggtgtgcac
     1681 cggacgcacg aagtgcacgt cgcttgcgcc ccaacgcatc agtttcggga tcggcagttt
     1741 cgccagcgaa gtcgcaacca tattcggcag tagtgcttca gtgctttcgc ccttcacatg
     1801 ggcgcgatac agcagccatt cgcctttatc ggtagtcaga cgctcagcct ggtcaacggt
     1861 aataccgcaa ccacgcgccc aaccttctgc cgctttgctc ggtttgcctt cagcgtcgaa
     1921 cgcctgggca atcgccgggc cgcgtttttc gatttcacga tctggttgcg cttccgccag
     1981 gttagctact ttcaacgcca gacgacgcgg agcagcaaac cattgaacgg tgccgtgtgc
     2041 gaggccagcg ttatccagct ccgcagtaaa gttcgcagca aaggactcag ccaggctgcg
     2101 cagtgctttt ggtggcagct cttcagtgcc gatttccacc agaaaagttt tctcagacat
     2161 agccgcctct tacttatctt tgttgcacat cgggaagccg agggcttcac gggaagcgta
     2221 gtatgcttct gccactgctt tggtcagggt gcgaatgcgc agaatgtagc gctgacgctc
     2281 agtgacggag atggctttac gcgcatccag caggttgaag ctgtgggcgg ctttcagaat
     2341 acgctcgtag gctggcagcg gcagcggatt ttccagcgcc agcagctgct gcgcttcttt
     2401 ctcgtactgc tcgaagcagg tgaacaggaa atccacatcc gcgtattcga agttgtaagt
     2461 ggactgctcc acttcgttct gatggaacac gtcgccgtag gtggttttac ccagcgggcc
     2521 gtcgctccag accaggtcgt aaacgctgtc tacgccctga atgtacatgg ccagacgttc
     2581 cagaccgtag gtgatctcgc cggtaaccgg tttacactcc agaccaccaa cctgctggaa
     2641 gtaagtgaac tgcgtcactt ccatgccgtt tagccacact tcccagccca gtccccaggc
     2701 acccagagtc gggttttccc agttatcttc cacgaaacgg atgtcgtgaa tagtcgggtc
     2761 catgcccagc tctttcagag aaccgaggta cagctcctga atattgtccg gcgatggctt
     2821 aatgaccacc tggaactgat agtagtgctg taaacggttg gggttttcgc cgtagcgacc
     2881 atcggtcggg cgacgagaag gctgaacata agcagccgcc atcggttctg gccccagcgc
     2941 gcgcagacag gtcattgggt gagaggttcc cgcgccgact tccatgtcca atggttgaac
     3001 aatggtgcag ccctggcgag cccagtaatc ctgtaaggtc aggatcaagc cctggaaggt
     3061 cctggtatca aacttttgca tattatttcg tgctggatac gtgtggattt aaaggaaggg
     3121 atcagtatac ccgctggatg gaagatatac agtacgaaac gggaaaaagc agggcttaac
     3181 gcatggaaag atgcaaaaaa tggccatctg cgtcaaaaga acagacaaac tgctctttac
     3241 ggaaggtata accgcgcatt tcatagcttc cctggaactg ttcgaaggcg gtgacgtcga
     3301 ttttttgctc gccggtgtta taacgttttg ccgcctgatc cttgcataac gcttccatat
     3361 tcaacgaacg cagaggatca acctttaccc gttgtgcttt ctgcacgggt gacggtgtag
     3421 accaacccac tagcaccata agcgccattg ccggaaagaa agcgttcatc atcattaaat
     3481 taccgtctgg tttgcaggtg agtcttatta tttatatcgg tatagtggct gatatcttac
     3541 gaatttcctt aagcctctat aagctgtagt gataaaactc agattattcc agtgcgccaa
     3601 ccaaatgatt tatcggtcag acttttttta accagataca gagacacaga tgcagcccaa
     3661 aatttactgg attgataacc tgcgagggat agcgtgttta atggtggtga tgattcacac
     3721 cactacctgg tatgtgacca atgctcatag tgttagcccc gtcacatggg atatcgccaa
     3781 tgttctgaat tctgcctctc gtgtcagcgt gccgctattt ttcatgattt ccggctatct
     3841 cttttttggc gaacgcagcg cccagccgcg ccatttcttg cgtatcggct tatgtctgat
     3901 tttttatagc gcaatcgcac tgctctacat tgcgctgttt acctccatca atatggagtt
     3961 agcgctgaaa aacctgctgc aaaagccagt gttttaccac ttgtggtttt tcttcgcgat
     4021 tgcggtgatt tatctggttt caccgctgat tcaggtgaag aacgtcggcg gaaaaatgtt
     4081 gctggtgcta atggcggtga ttggcattat cgctaaccca aacacagtgc cgcagaaaat
     4141 tgacggtttt gaatggctgc caattaactt atatatcaat ggcgatactt tttactacat
     4201 tctgtatggc atgttgggcc gcgctatagg gatgatggac acacagcata aagcactgtc
     4261 gtgggtgagc gccgcgctgt ttgcgacggg ggtttttatt atctctcgcg ggacattata
     4321 tgaattgcag tggcgcggaa attttgccga tacctggtat ctttactgtg ggccgatggt
     4381 ttttatctgc gcaatcgcgc tattgactct ggttaaaaac acgctggata cgcgtaccat
     4441 tcgcggactt ggcttaatct cccgccattc attgggtata tacggattcc acgccttgat
     4501 tatccatgcg ctgcgcaccc ggggaattga gcttaaaaat tggccaatac tggatattat
     4561 ttggatcttt tgcgcgacgt tggcagcgag tttgttactt tctatgctgg tacaacgaat
     4621 cgacagaaac agattagtga gttaagtaaa agcccgttca cattggactg accgggctta
     4681 cgtgaattat tcgctgtatt cttcctgggt aacctgtgtt tcttttggcg ggtttattcc
     4741 ggcatcacga atattcttct gcaccgctac tgcaccaaac aagcttaaga agaaagccag
     4801 tccataaaaa cctttttcac tgagtaataa tgtcgcattc cacagaccta
//
LOCUS       DAZPDU010000252         4844 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZPDU010000252 DAZPDU010000000
VERSION     DAZPDU010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4844
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.256"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(34..954)
                     /locus_tag="ACHURS_004209"
     CDS             complement(34..954)
                     /locus_tag="ACHURS_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR1239800.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVVSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAVMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITFDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1092..1991
                     /gene="pgrR"
                     /locus_tag="ACHURS_004210"
     CDS             1092..1991
                     /gene="pgrR"
                     /locus_tag="ACHURS_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFR1239801.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            2328..3941
                     /gene="mppA"
                     /locus_tag="ACHURS_004211"
     CDS             2328..3941
                     /gene="mppA"
                     /locus_tag="ACHURS_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFR1239802.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKDRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(3992..>4844)
                     /gene="ynaI"
                     /locus_tag="ACHURS_004212"
     CDS             complement(3992..>4844)
                     /gene="ynaI"
                     /locus_tag="ACHURS_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFR1239803.1"
                     /translation="INYVIENYKLKFITPDVIDFICTSLIAVILTIKLFLLINQFEKQ
                     QIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKD
                     ILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSL
                     FSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYF
                     NQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDNI
                     TPPEQGR"
BASE COUNT         1307 a         1081 c         1140 g         1316 t
ORIGIN      
        1 ccatatcact attgcggatt tgaatatgtt tcattacaga gtttcctcaa agaacggagc
       61 taatactgaa attgcttcag ccacataggc tttgccgtca taaagatcca tatggtttgc
      121 gccttcaaca atgtgatagc gtttatcttg gcttgatgct cgatcgtaca ggtcgtcact
      181 catccatttg ctccctgcct ggctgcccgc cacaatctgc atcggctgag tcaggtacac
      241 ttccgccata tggtaagcat caaaggtaat aatctggtta aggctgcgca aagtagcgta
      301 acccggtgct gttggatact gcgcgcgagg ggtgtggtaa tactcccagg cctgacgcag
      361 ttcttcgttc ggcgcatcgg actccttcat tggtgccagt ggcataacgg catattctcc
      421 gctgctaata tcgctggttc gggcgtttga acccgcttca acgtatggca gggcatcaat
      481 agatttcaca ttgttttccc aaccattacg gaacatcgaa ccaatattga ccgcactaac
      541 ggtaccgatg gccttgatgc ggcgatcctg aattgcagca ttggctgtat atcctgcacc
      601 ggcacaaatt cccatcgcac caattcgggt attgtcgaca taagaaagcg ttgtcaggta
      661 atcaatcacg gcactgatgt cttcagtacg aatgtatggg ttttctaact gacgcggctc
      721 gccgccactt tcaccttgat aagatgcgtc ataagcaata gtgacaaatc ccttttccgc
      781 cagttttttg gcataggttc cggccgtttg ttctttaacg cccccacctg ggtgagatac
      841 cacaattgcc tgatactgac gggtttcatc aaattttgga gggaaataga tcactgcaga
      901 caaagagatg gttggattat tgctgttagt gaagctgact ttattattca tcattccgtt
      961 cctcatggag ttgtcggttc gttttaacgg ttggtgatat cactatagat attgatcatt
     1021 aagttgatta gaccctaaat tatgattaga cctatcattt aaatgattaa tagattgcat
     1081 ggagatactg aatgaagcgt gaagaaatcg ctgatctgat ggcgtttgtc gtcgttgcag
     1141 aggagcgtag cttcactcgt gcagcagccc gcctgagcat ggcgcagtca gctttaagcc
     1201 agatagtgcg tcgtatagaa gaacgattgg gattgcggct tctgacgcga accacgcgca
     1261 gcgttgttcc aactgaagcg ggcgagcatc ttttgtctgt tcttggccct atgttgcatg
     1321 acatagattc agccctggca tccctgagcg agctgcagaa ccgcccatcc gggacaatac
     1381 gtattactac tgtagaacat gcagccaaaa cgatattgtt accagcaatg cgcacattcc
     1441 tgaaatcgca tcctgaaatt gatattcagc tcaccattga ttatggtttg accgatgtcg
     1501 tttctgaacg ttttgatgca ggcgtccgtc tgggtgggga gatggataaa gatatgatcg
     1561 ccattcgaat cgggccagat ataccaatgg ctattgttgg ctcaccggat tatttttctc
     1621 gccgaagtgt tccaacgtca gtgtcacaat taatagatca tcaggcaatt aatttgtatc
     1681 ttcccacatc gggtacagca aatcgctgga gattaatacg cggtggacgt gaagttcgtg
     1741 ttcgcatgga aggtcagctt ttactgaata cgatagacct gatcattgat gctgcaattg
     1801 atgggcatgg attggcgtat ctaccttatg atcaggttga gcgggctatt aaagaaaaaa
     1861 aactgatacg tgttttggat aaattcacac cagatttacc cggttatcac ctgtactatc
     1921 cacaccgtcg acatgctggc tcggcattct cattatttat agataggctg aagtataaag
     1981 gtgctgttta gcactacttg ctgatacatt aatttaattt ttctcttaac gtattctcag
     2041 ttcctttcaa cgttttggtt attttttatt cttcgtacaa tggcgacaga tgctgattat
     2101 gataccgaaa actggtttga acgtgcgaag cctgaacgta gtgttcggtg ttctatgtgc
     2161 tttaccgcat tttgtcgtct attatttaca ctaaatctga tttaatatat tgatacataa
     2221 aacattttat gctccctgtg tcactttttt gatggaagtt gtttgcattt ctttaaggcg
     2281 aaacaaataa ttacgcatca attttaatgt cggttagagg gaaacttatg aagcactctg
     2341 tttcagtcac gtgttgtgcg ctgttggtca gcagcatttc tctttcgtat gctgcagaag
     2401 ttccgagcgg cacagtactg gcagagaagc aggagctggt gcgccacatt aaagatgagc
     2461 ctgcgtcgct ggatcccgct aaagccgtgg gcctgccaga gattcaggtc attcgcgatc
     2521 tgtttgaagg actggtgaat cagaacgaga aaggggagat tgtccccggc gttgcgactc
     2581 agtggaaaag taatgacaac cgtatctgga cttttaccct gcgcgataac gcaaaatggg
     2641 cggatggcac accggtaacg gcgcaagatt ttgtctacag ctggcagcgt ctggtggacc
     2701 cgaaaacgct atcgccattt gcatggtttg ccgcgctggc gggaatcaac aacgcacagg
     2761 cgattattga tggtaaagct acgccagacc agcttggcgt caccgcagtt gatgcccata
     2821 ctttgaaaat tcagcttgat aaaccgttgc cgtggtttgt gaatttaacc gctaactttg
     2881 ccttcttccc ggtgcaaaaa gccaacgtag aaagcggtaa agagtggacg aaacccggaa
     2941 atctgatcgg caatggcgct tatgttctta aagatcgcgt agtcaatgaa aaactggtcg
     3001 tggtaccgaa tacccattat tgggataacg ccaaaacggt actgcaaaaa gtgaccttcc
     3061 tgccaattaa tcaggaatcc gcagccacta agcgttacct cgcgggggat attgatatca
     3121 ccgaatcctt cccgaaaaat atgtatcaga agctgttgaa ggatattccg gggcaggttt
     3181 atacgccgcc gcagctcggg acctattatt atgcgtttaa cacgcaaaaa gggccgacgg
     3241 cagatcagcg cgttcgtctg gcattaagta tgacgataga tcgccgcctg atgaccgaaa
     3301 aagtattagg gacgggcgaa aagccagcgt ggcattttac accagatgtt accgccggat
     3361 ttacgccgga accttcgccg tttgaacaaa tgagtcagga agaactgaat gcgcaggcaa
     3421 aaactttgtt gagcgcagct ggttatggtc cgcaaaaacc gctgaagctg acgcttttgt
     3481 ataacacttc agaaaaccat caaaaaattg cgattgctgt agcatcgatg tggaaaaaga
     3541 accttggcgt agatgttaaa ttgcaaaatc aggaatggaa aacctatatc gatagccgta
     3601 acaccggcaa ttttgatgtt atccgcgcct catgggtggg ggattataat gaaccctcca
     3661 ctttcctgac attattaacg tcaacgcatt caggaaatat ttcacgcttt aacaatccgg
     3721 catacgacaa agttctggcc caggcatcga cggaaaatac cgttaaagcg cgtaatgccg
     3781 attacaacgc ggcagaaaaa atcctcatgg agcaagcacc gattgcacca atttatcaat
     3841 ataccaatgg acgattaatc aagccgtggc tgaaaggtta tcccattaat aacccggaag
     3901 atgtggcgta cagccggact atgtatattg tgaagcattg atgtgatggg aactggcgtt
     3961 acccttgtgg ataacgccag tgatgtctga tttagcgccc ctgttccggc ggtgtaatgt
     4021 tatccatata cagcgtctgg ctggggaagg caaagtccgc gccgtgtgac tgcacaatat
     4081 cgataatctt caaataaacg tcttgctgtg cagcaagcca ttcagcccat accgtggttt
     4141 tggtaaagca ataaaccata atattcaatg acgagtcggc aaactggttg aaataaacca
     4201 gtaaggtttg tcgctggtcg atggccgggt gatttttcag catctcacgt acggcttcga
     4261 caataacgcc cacttttgcc gcatcctcat aacgtaaacc aatggtcgtg gtaatgcggc
     4321 ggttggtcat tcgtcctggg ttttctacgc tgatcgacga aaacagcgag ttcggtacgt
     4381 acaatggacg attatcaaag gtcgtaattt tggtaattcg ccagccaatt tccgctactg
     4441 taccttcgat atttctgtcc ggtgaacgga tccagtcgcc aatactgaaa ggacggtcga
     4501 aatagagcat gatcccggaa aagaaattac tcagaatatc tttaccggcc ataccgacag
     4561 ccagaccacc aataccacca aaggtcagca agccagaaag gctcatgccg aaatgttcgc
     4621 cataaagcag aacaagcacc acaataatgg tgattttgat aatgcgcgac ataatccgcg
     4681 cactggtgat atcgcgacct tttttaatct gctgtttttc aaactgatta atcagcaaaa
     4741 atagcttaat cgtcagaata accgcaatca gggacgtaca gataaaatcg ataacgtctg
     4801 gggtgataaa tttgagttta tagttttcta taacataatt aata
//
LOCUS       DAZPDU010000253         4833 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZPDU010000253 DAZPDU010000000
VERSION     DAZPDU010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4833
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.257"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(108..551)
                     /locus_tag="ACHURS_004213"
     CDS             complement(108..551)
                     /locus_tag="ACHURS_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFR1239804.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEVLVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVEE"
     gene            708..1637
                     /gene="metA"
                     /locus_tag="ACHURS_004214"
     CDS             708..1637
                     /gene="metA"
                     /locus_tag="ACHURS_004214"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFR1239805.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            1906..3507
                     /gene="aceB"
                     /locus_tag="ACHURS_004215"
                     /pseudo
     CDS             1906..3507
                     /gene="aceB"
                     /locus_tag="ACHURS_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            3537..4828
                     /gene="aceA"
                     /locus_tag="ACHURS_004216"
                     /pseudo
     CDS             3537..4828
                     /gene="aceA"
                     /locus_tag="ACHURS_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
BASE COUNT         1205 a         1272 c         1225 g         1131 t
ORIGIN      
        1 gcagaaggcc atcctgacgg atggcctttt tgcattggcg cagaaaaaaa tgccgggtgc
       61 gacgctggcg cgtcttctcc agcctgggca ggcacggcag gggcagctta ttcctccaca
      121 tacgccagat tcagcaacgg atacggtttc cccaaatcgt ccacctcaga gcgtcccgta
      181 accttaaaac ccaccttctt atagaaccca accgcctgct cattttgctc attaacgttg
      241 gttgtcagtt ccggtgccat cgagagcgca tgctccacca gcatccgacc cacgccgcag
      301 ccgcacacat caggatcgat aaacagcgca tccatatgct gcccacttaa caacataaac
      361 ccaaccggct gatcccgttc attaaccgcg acccacaacg gcgcttccgg caggaaggaa
      421 cgaactagca cctccagctc ggcccgatac tctgctgata aaaaatcgtg tgtggcatcg
      481 acagaacgac accaaatcgc aacgagttcc tccccttcct catgccgtga gcggcgaata
      541 ctaataacca ttttctctcc ttttcgtcat tcttatattc taacgtagcc ttttccttga
      601 aactttctca ccttcaacat gcaggctcga cattggcaaa ttttctggtt atcttcagct
      661 atctggatgt ctaaacgtat aagcgtatgt agtgaggtaa tcaggttatg ccgattcgtg
      721 tgccggacga gctacccgcc gtcaatttct tgcgtgaaga aaacgtcttt gtgatgacaa
      781 cttctcgtgc gtctggtcag gaaattcgtc cacttaaggt tctgatcctt aacctgatgc
      841 cgaagaagat tgaaactgaa aatcagtttc tgcgcctgct ttcaaactca cctttgcagg
      901 tcgatattca gctgttgcgc atcgattccc gtgaatcgcg caacacgccc gcagagcatc
      961 tgaacaactt ctactgtaac tttgaagata ttcaggaaca aaactttgac ggtctgatag
     1021 ttaccggcgc gccgctgggc ctagtggagt ttaatgatgt cgcttactgg ccgcagatca
     1081 aacaggtgct ggagtggtcg aaagatcacg tcacctcgac gctgtttgtc tgctgggcgg
     1141 tacaggccgc gctcaatatc ctctacggca ttcctaagca aactcgcacc gacaaactct
     1201 ctggcgttta cgagcatcat attctccatc ctcatgcgct tctgacgcgt ggctttgatg
     1261 attcattcct ggcaccgcat tcgcgctatg ctgactttcc ggcagcgttg attcgtgatt
     1321 acaccgatct ggaaattctg gcagagacgg aagaagggga tgcatatctg tttgccagta
     1381 aagataagcg cattgccttt gtgacgggcc atcccgaata tgatgcgcaa acgctggcgc
     1441 aggaatattt ccgcgatgtg gaagccggac tagacccgga tgtaccgtat aactatttcc
     1501 cgcacaatga tccgcaaaac aaaccgcgcg cgagctggcg tagtcacggt aatttgctgt
     1561 ttaccaactg gctcaactat tacgtctacc agatcacgcc atacgatcta cggcacatga
     1621 atccaacgct ggattaatct tctgtgatag tcgatcgtta agcgattcag caccttacct
     1681 caggcacctt cgggtgcctt ttttatttcc gaaacgcacc tcagcaagtg aataaatttt
     1741 attcatattg ttatcaataa gttatcaagt atttttaatt aaaatggaaa ttgtttttga
     1801 ttttgcattt taaatgagta gtcttagttg tgctgaacga aaagcgcaca acgatccttc
     1861 gttcacagtg gggaagtttt cggatccatg acgaggagct gcacgatgac tgaacaggca
     1921 acaacaaccg atgaactggc tttcacaagg ccgtatggcg agcaggagaa gcaaattctt
     1981 actgccgaag cggtagaatt tctgactgag ctggtgacgc attttacgcc acaacgcaat
     2041 aaacttctgg cagcgcgcat tcagcagcag caagatattg ataacggaac gttgcctgat
     2101 tttatttcgg aaacagcttc cattcgcgat gctgattgga aaattcgcgg gattcctgcg
     2161 gacttagaag accgccgcgt agagataact ggcccggtag agcgcaagat ggtgatcaac
     2221 gcgctcaacg ccaatgtgaa agtctttatg gccgatttcg aagattcact ggccccggac
     2281 tggaacaaag tgatcgacgg gcaaattaac ctgcgtgatg cagttaacgg caccatcagc
     2341 tataccaatg aagcaggcaa aatttatcag ctcaagccca atccagcggt tttgatttgt
     2401 cgggtacgcg gtctacactt gccggaaaaa catgtcacct ggcgtggtga ggcaatcccc
     2461 ggtagcctgt ttgattttgc gctctatttc ttccacaact atcaggcact gttggcaaag
     2521 ggcagcggtc cctatttcta tctgccgaaa acccagtcct ggcaggaagc ggcctggtgg
     2581 agcgaagtct tcagctatgc agaagatcgc tttaatctgc cgcgcggcgc catcaaggcg
     2641 acgttgctga ttgaaacgct gcccgccgtg ttccagatgg acgaaatcct tcacgcgctg
     2701 cgcgaccata ttgttggtct gaactgcggt cgctgggatt acatcttcag ctatatcaaa
     2761 acgttgaaaa actatcccga tcgtgtcctg ccagacagac aggccgtgac gatggataaa
     2821 ccattcctga atgcttactc acgcctgctg attaaaacct gccataaacg cggtgctttt
     2881 gcgatgggcg gcatggcagc gtttattccg agcaaagatg aagagcgtaa taaccaggtg
     2941 ctcaacaaag taaaagcgga taaagcgctg gaagtcaata acggtcacga tggcacatgg
     3001 atcgctcacc caggccttgc ggacacggca atggcggtat tcaacgacat tctcggctcc
     3061 cgtaaaaatc agcttgaagt attgcgcgaa caagacgcgc cgattactgc cgatcagctg
     3121 ctggcacctt gtgacggtga acgcaccgaa gaaggtatgc gcgccaacat tcgcgtggct
     3181 gtgcagtaca tcgaagcgtg gatctccggc aacggctgcg tgccgattta tggcctgatg
     3241 gaagatgcgg cgacggctga aatttcccgt acctcgatct ggcagtggat ccatcatcaa
     3301 aaaacgttga gcaatggcaa accggtgacc aaagccttgt tccgctagat gctgggcgaa
     3361 gagatgaaag tcattgccag cgaactgggc gaagaacgtt tctcccaggg ccgttttgac
     3421 gatgccgcac gcttgatgga acagatcacc acttccgatg agttaattga tttcctgacc
     3481 ctgccaggct accgcctgtt agcgtaaacc accacataac tatggagcat ctgcacatga
     3541 aaacccgtac acaacaaatt gaagaattac agaaagagtg gactcaaccg cgttgggaag
     3601 gcattactcg cccatacagt gcggaagatg tggtgaaatt acgcggttca gtcaatcctg
     3661 aatgcacgct ggcgcaactg ggcgcagcga aaatgtggcg tctgctgcac ggtgagtcga
     3721 aaaaaggcta catcaacagc ctcggtgcac tgactggcgg tcaggcgctg caacaggcga
     3781 aagcgggtat tgaagcagtc tatctgtcgg gatggcaggt agcggcggac gctaacctgg
     3841 cggccagcat gtatccggat cagtcgctct atccggcaaa ctcggtgcca gctgtggtgg
     3901 agcggatcaa caacaccttc cgtcgtgccg atcagatcca atggtccgcg ggcattgagc
     3961 cgggcgatcc gcgctatgtc gattacttcc tgccgatcgt tgccgatgcg gaagccggtt
     4021 ttggcggtgt cctgaatgcc tttgaactga tgaaagcgat gattgaagcc ggtgcagcgg
     4081 cagttcactt cgaagatcag ctggcgtcag tgaagaaatg cggtcacatg ggcggcaaag
     4141 ttttggtgcc aactcaagaa gctattcaga aactggtcgc ggcgcgtctg gcagctgacg
     4201 tgacgggcgt tccaacccta ctggttgctc gtaccgatgc cgatgcagcg gatctgatca
     4261 cctccgattg cgacccgtat gacagcgaat ttattaccgg cgagcgtacc agtgaaggct
     4321 tcttccgtac tcatgcgggc attgagcaag cgatcagccg tggcctggcg tatgcgccat
     4381 atgctgacct ggtctggtgt gaaacctcca cgccggatct ggaactggcg cgtcgctttg
     4441 cacaagctat ccacgcgaaa tatccgggca aactgctggc ttataactgc tcgccgtcgt
     4501 tcaactggca gaaaaacctc gacgacaaaa ctattgccag cttccagcag cagctgtcgg
     4561 atatgggcta caagttccag ttcatcaccc tggcaggtat ccacagcatg tggttcaaca
     4621 tgtttgacct ggcaaacgcc tatgcccagg gcgagggtat gaagcactac gttgagaaag
     4681 tgcagcagcc ggaatttgcc gctatacctt cgtatctcac cagcaggaag tgggtacagg
     4741 ttacttcgat aaagtgacga ctattattca gggcggcacg tcttcagtca ccgcgctgac
     4801 cggctccact gaagaatcgc agttctaagc aac
//
LOCUS       DAZPDU010000254         4809 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZPDU010000254 DAZPDU010000000
VERSION     DAZPDU010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4809
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.258"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(148..3369)
                     /gene="carB"
                     /locus_tag="ACHURS_004217"
     CDS             complement(148..3369)
                     /gene="carB"
                     /locus_tag="ACHURS_004217"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFR1239806.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(3387..4535)
                     /gene="carA"
                     /locus_tag="ACHURS_004218"
     CDS             complement(3387..4535)
                     /gene="carA"
                     /locus_tag="ACHURS_004218"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFR1239807.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT         1061 a         1349 c         1242 g         1157 t
ORIGIN      
        1 agtgtgagct aaatcctgct tatgtcatca aaaatatcga ttccccaaaa gatttactta
       61 atgggcaaaa aaaacggtcc tcatcagaga accgtattac ttattcgatc aaattagcgg
      121 atgaaaaata tccgccatga cacgctatta tttgatctgc gcgtgcattt cctgcaccga
      181 aattactttt tcggtcgcgt cggcattcag cgccatcgcg gtagcgaaac caccgttcag
      241 ggtggtgtca taatgcactt tatattgcag cgcactgcga cggatcacgc gggagtcttc
      301 aatcgcgcga cggcctgaag tggtgttgat gatgtaggta tattcgccat tcttgatgcg
      361 gtcctgaatg tgcggacggc cttcatgcac cttgttcacc agacgcggat taatgcccgc
      421 ttcgcccagc acaatcgccg tgccgtgggt cgcatccagc tcgaagccct gtttcagcag
      481 ttttgccgcc aggtccacca cgcgttcttt atcgccttcg cgcacggaaa gcaacgcacg
      541 accgtgtttc ttcatggtgg agttgctgcc cagctgcgct ttggcaaacg cttcagcgaa
      601 ggtgcggccc acgcccatca cttccccggt ggagcgcatt tctggcccta acagcgggtc
      661 aacacccggg aatttattga acggcagcac cacttctttc accgagtagt acggcgggat
      721 aacttctttg gttacgccct gctcagccag cgatttgcca gccatcacac gcgccgccac
      781 ttttgccagc ggtacgccgg tggctttgga gacgaacgga acggtacgcg ccgcacgcgg
      841 gttaacttca atcaggtaga cttcgttgtt tttcaccgca aactgcacgt tcatcagacc
      901 gcgcacctgc aattcgaagg ccagtttctg cacctgctgg cgcatcacat cctgaatttc
      961 ctgacttaag gtgtaggctg gcagagaaca tgcggagtca ccggagtgca cgcccgcctg
     1021 ctcgatgtgc tccatgatgc cgccaatcag caccatttcg ccgtcgcaga tggcatccac
     1081 gtcaacttct accgcgtcat caaggaagtg atccagcaac actggcgcat cgttagacac
     1141 gctgaccgcc gtctggaagt agcgacgcag gtcagcttcg tcatagacga tttccatcgc
     1201 ccgaccgccg agaacgtaag acggacgtac caccagtggg tagccaatct ctttcgcctt
     1261 ctctaccgcc atttcaatag cggtaacggt ggcgttcgcc ggttgtttca gtttcagacg
     1321 ctcaaccgca tgctggaagc gttcacggtc ttctgcacgg tcgatagcat ccgggctggt
     1381 gccgataacc ggtacgccag cagcttccag cgcgcgcgcc agtttcagcg gggtctgacc
     1441 gccgtactga acgataacgc ctttcggctt ctcaatacgc acgatttcca gcacatcttc
     1501 cagagttacc ggctcgaagt agaggcgatc ggaagtgtcg tagtcggtgg agacggtttc
     1561 cgggttacag ttaaccataa tggtttcgta accgtcttcg cgcagcgcca gcgaggcgtg
     1621 tacacagcag tagtcgaatt cgataccctg accgatacgg ttcgggccgc cgccgagaac
     1681 catgattttt tcacggtcgg tagacggatt cgcttcgcac tcttcttcat aagtggagta
     1741 catgtaagcg gtgtcggtgg cgaactctgc cgcacaggta tccacgcgct tataaaccgg
     1801 gtgcaggtca tactggtcgc gcagcttgcg gatttccgct tcgcgcacgc ccgccagttt
     1861 tgccagacgc gcatcggcaa agcctttgcg tttcagctgg cgcaggaatt cagcgttcag
     1921 gccagtgatg cccacttccg ctactttctc ttccagacgc accagttctt caatctgtac
     1981 caggaaccag cggtcaatat tggtcaggtt gaacacgccg tccacagaca ggcccgcacg
     2041 gaaggcatcg gcgatgtacc agatacgctc tgcgccagca tctttcagtt cgcgacggat
     2101 tttggttaac gcttccgggt catcgaggct cactttcggg tcgaatccag tcgcaccgac
     2161 ttccaggccg cgcagcgctt tttgcaggga ttcctgctgc gtgcgaccaa tcgccatcac
     2221 ttcgccaacc gatttcatct gagtggtcag acggtcgtta gcaccagcga atttttcgaa
     2281 gttgaagcga ggaattttag taaccacata gtcgatggac ggctcgaagg aggccggagt
     2341 acgtccgcca gtgatgtcgt tcatcagttc gtcgagggtg taacccaccg ccagtttcgc
     2401 cgccacttta gcaatcggga aaccggtcgc tttcgacgcc agcgccgaag aacgggacac
     2461 gcgtgggttc atttcgataa caatcaggcg accgtttttc gggttcaccg caaactggac
     2521 attggaacca ccggtttcaa cgccgatttc acgcagcacc gccatcgagg cgttacgcat
     2581 gatttgatat tctttgtcgg tcagcgtttg ggctggcgcg acagtgatgg agtcaccggt
     2641 atggatgccc atcgcatcaa agttttcgat agagcagacg atgatgcagt tgtcgttttt
     2701 atcacgcacc acttccatct cgtactcttt ccagccgatc agcgactcat caatcagcag
     2761 ctctttggtt ggggagagat ccagaccgcg ggcgcaaatt tcttcaaact cttcgcggtt
     2821 ataagcgata ccgccgccgc taccgcccat ggtaaaggat gggcgaataa tgcacgggaa
     2881 gcccacgtca gcggcaaccg ccagcgcttc ttccatcgtg tgtgcgatac cggaacgcgc
     2941 ggtttccaga ccaattttct tcatcgctac gtcgaaacga cggcggtctt ctgctttatc
     3001 aatcgcatcg gcggtcgcac caatcatagt cacgccgaac tcttccaaca cgccctgacg
     3061 ctccagctcc agcgcgcagt tcagcgccgt ctgaccgccc atcgttggca gcaccgcgtc
     3121 cgggcgctct ttttcaataa tcttgcgtac tacttcccag tgaatcggct cgatgtaggt
     3181 cgcatcggcc atttccgggt cggtcatgat ggtcgccggg ttggagttca ccagaatgac
     3241 gcggtaaccc tcttcgcgca gggctttaca tgcttgcgcg ccagagtagt caaactcaca
     3301 cgcctgaccg ataacaatcg ggcccgcacc cagaatcagg atacttttta tatctgtacg
     3361 ttttggcatg gctcttttac tcctgattac ttagcggttt tacggtactg ctcaattaac
     3421 tcgataaagt ggtcgaacaa cggcgctgcg tcgtgtggcc cagggctggc ttccgggtga
     3481 ccctggaagc tgaacgccgg tttatcagtg cgatgaatgc cctgtaacgt accgtcgaac
     3541 agggatttat gcgtgacacg caggtttgca ggtaatgttg cttcatccac cgcaaaaccg
     3601 tggttctggg cggtgatcat caccacgttt ttctccacat ctttaaccgg atggttgccg
     3661 ccgtggtgac caaatttcat tttgacagtc ttcgcaccgc tcgccagcgc cagcagctga
     3721 tgaccgaggc agatgccgaa taccggaata tcggtttcga ggaatttctg gatggcggta
     3781 atggcgtaat cgcacggggc cgggtcgcca ggaccgttgg agaggaagat gccgtctgga
     3841 ttcattttca gcacatcttc cgcagaagtt tgcgccggaa cgatggtcag gcgacagcct
     3901 ctatccacca gcatccgcag gatgttgcgc ttggcaccaa aatcataagc cacgacgtgg
     3961 aacggcagct cgtcttcttt tttcgcttct ggcaggccgc cggtcaacgt ccagctccct
     4021 tgtgtccagc tataggcttc tgcggtggtc acttcttttg ccagatccat gccattcaga
     4081 cctgggaacg cgcgggcttt ttctaacgcc aacgccgcat ccgggttatc gcctgcgata
     4141 atgcagccat tctgtgcgcc tttctcgcgc agtaaacgcg tcagcttacg ggtatcgata
     4201 tcggcaatcg ccacgatgtt atggcgcttc aggtaagaag agaggtcttc ggtattacgg
     4261 aagttgctgg caatcagcgg cagatcgcga atcaccagac cttgtgcatg tacctgagaa
     4321 gattcttcat cggcgtcatt agtgccgaca ttgccaatat ggggataagt aagagtaacg
     4381 atttgacgag aataggaagg atcagtgagg atttcttgat aaccggtcat tgaagtattg
     4441 aaaacgactt ccccaaccgc cgaacctgtt gcccctatgg cccgaccgtg aaactgggtt
     4501 ccgtcttcca gaaccaatag cgctgactta atcaaaacac cctccagaga atattcactc
     4561 actttatttg catattaatt cacaatgatg acgtaaatca atgcaaatct gcttaccgac
     4621 gaatttctgg caaacggcgg cattctggag atatgggcgc taaaagtcaa cttaatggcg
     4681 gtatttttta tattattttg tggctcatgc atgttttaca ggcattaagc gacaaaaaga
     4741 tcatataacc tgttctggaa aacgcttgcg cagcaagaag attgcatgaa ataaaaacga
     4801 gcaggaaaa
//
LOCUS       DAZPDU010000255         4803 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZPDU010000255 DAZPDU010000000
VERSION     DAZPDU010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.259"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            6..81
                     /locus_tag="ACHURS_004219"
     tRNA            6..81
                     /locus_tag="ACHURS_004219"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="ACHURS_004220"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="ACHURS_004220"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(299..1057)
                     /locus_tag="ACHURS_004221"
     CDS             complement(299..1057)
                     /locus_tag="ACHURS_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFR1239808.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQLGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1065..2168)
                     /locus_tag="ACHURS_004222"
     CDS             complement(1065..2168)
                     /locus_tag="ACHURS_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFR1239809.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTNIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2178..3359)
                     /locus_tag="ACHURS_004223"
                     /pseudo
     CDS             complement(2178..3359)
                     /locus_tag="ACHURS_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3427..4459)
                     /locus_tag="ACHURS_004224"
                     /pseudo
     CDS             complement(3427..4459)
                     /locus_tag="ACHURS_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
BASE COUNT         1317 a         1241 c         1147 g         1098 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt agggtctccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcga aaggccatcc gaaaggatgg cctttttgct tttcgaacta acattcaatt
      301 aatggattac ctgcgataaa aatgccctcg tacgctctga tttaggatgc gcaaaaaatt
      361 catcaggtgc agcttgctcc actatttccc cacgatccat aaaaatcacc cggtcagcga
      421 cggttcgcgc aaaccccatc tcatgcgtta cacacaacat tgtcataccc gattgcgcca
      481 gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc gacgttggct
      541 catcaaacaa cataattttc ggcttcatac acagcgaacg cgcaatggca acgcgttgct
      601 gctgaccacc agaaatttgt ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct
      661 caaggtaatg catcgccaga gcttcagcct ctttctttgg catcttgcgt acccaaatcg
      721 gtgccagggt acagttctgt agaacggtca gatgagggaa gagattgaaa tgctgaaaga
      781 ccattcccac ttcctgcctg acgcgctcaa tattgcggat atcttcatta agttcgatgc
      841 catctactac gatccgtccc tgttgatgtt cttccagatg attaatacaa cgaatggttg
      901 tcgatttacc ggaaccagaa gggccacaca gaacgatccg ttctcccagt tgcacggtta
      961 aatttatatt tttcaaaaca tggaattgtc cataccattt attgacgttt tccagcgtaa
     1021 tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc
     1081 ccggtgttaa aacgtttttc cagatgctgg ctatagcgcg acatgctgaa acagaagatc
     1141 cagtagatca gtgcggcgaa aacataccct tccgtcgaca tacccaacca ggcgggatca
     1201 acggttgcct gctgaacgct actgaaaaga tcgaacaacc cgataatgat caccaggctg
     1261 gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag cttcaacgcc
     1321 tgtggcagaa taaccagccc ctgagttttc cagtaaccca atgccagtga ctctgccgct
     1381 tcatattgcc ctttaggcag cgcctgtaat ccgcctcgca cgacttccgc aacatatgct
     1441 gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatatt ggtgccttct
     1501 gccataaaca aaggcagcat gaccgaagac ataaacaata cggtaatcag cggtacgccg
     1561 cgccagaact cgataaaaat gaccgataag atacgcacaa tcggcatatg ggagcggcga
     1621 cctaacgcca gtaagatccc ccacggtagc gcccccgcaa tcccaacgga tgcaataatt
     1681 aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctccagacc aaaaaaaccg
     1741 ccatacatca gccaccagac aatcagtggg taaatcaccg cccagaccgc aatatagcga
     1801 ccgcgatgcg ggagtatttt ccagaacatt ggtgcgattg acacaaggcc aatcagtaat
     1861 gccaggttaa ttcgccagcg ttggtcgtgt gggtaaagtc catacataaa ctgaccaaat
     1921 cgttcgtgga tgaagaccca acaggcaccg gcttttgtgc agtcggcacg cgttgagcca
     1981 acccaattag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc ccacatcaac
     2041 catatgcagc caatagtcag caggctattg ctccagctgg agaacagatt ttttcgcacc
     2101 cacaccatcg cgcggcttga gttatggctc gctgggcgcg agggctgaga cagcaatact
     2161 tttgtcatca tgactcctta gcgttcaacg atcgcgatgc ggcggttata gatattcatc
     2221 agcagcgaga tagtcaggct gataatcaga tagaccgaca tggtcatggc tatcgtctcg
     2281 atggcttgcc ccgtctggtt cagcacggtg ccagcaaaca gcgaaaccat atcgggatag
     2341 ccaatagcgg cggcaagaga ggagtttttg acgatgttga gatactggct ggttaacggc
     2401 ggaataatca ctcgcagtgc ctggggaata atgacctagc gtagcgtaac cgggttgggt
     2461 aatcccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat cccagcgcgg
     2521 ataatctcgg cgataaatgc agatgtataa accgaaagtg ccagcgttaa ggctgccagt
     2581 tcaggaatta aaaccatccc gccgcggaag ttaaagcctc gtagggctgg gacatcccag
     2641 tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccaatgat caacactgcg
     2701 gcgatcggcc aggtgcggcg cagttgtccg gtctttatct ggtatgtttt attaaagcgg
     2761 aatagcccaa cagaaaggac tatagccata acaacagcca ggataaacgc aataaatccg
     2821 tctcccagct gcggtgacgg aatataaagc ccacgattgc tcaaaaaggc cagatcaaac
     2881 gcgctcacag cctggcgtgg tccgggcaaa ttgcgcaaca cggcaaagta ccagaagaag
     2941 atttgcagca gcgggggaat attacggaag atctcaatat aaattgtgga aagctttcgt
     3001 agcagccagt tatccgaaag tctcgccaga ccgataaaga agcccaaaac agaagcgaac
     3061 acaatacaca atgcagaaac cagtagcgta ttgagtaagc cgacaataaa aacgcgtccg
     3121 taggtgtcgc cctgctggta atcgatcaaa tgctggacaa taccgaagcc agcgccgcga
     3181 tccagaaagg caaaacctga agtaatgcca cgattattga gattcgttac agtgttgtga
     3241 aataaccaac caacaatgcc gacaacagca acaacggcaa ggatctggaa taaccaggca
     3301 cgaaccgtag ggttggcaaa agagagtgag cctttaacgg ttgagcggcg atgagacata
     3361 gaaacctcgg aaagcataac gctgaagaga gcgccgccgt agcgacgctc gttacctcac
     3421 accgacttaa cgcacgggcg gcgcgtactg aataccgccg ttattccaga gattattttg
     3481 cccacgttta attttcagcg ggctttctga acctacgtta cgctcgaaaa tttccgagta
     3541 gttacccacc tgtttgatga tgttataggc ccatttatta tccagcttca gatccttgcc
     3601 gtaatcgcct tctttaccca gcagatgtgc catatcaggc gttgctggat tagccgcttt
     3661 ttcatcgacg ttctgggaat tgatgcccat ctcttcagca ttcagcattc agcatggcga
     3721 aaagcgtcca gcgtacaatc gagaaccatt catcatcgcc acgacgaact accggcccaa
     3781 gcggttcttt agagataacc tccggtaaga caatccattc agctgggttg cttaatttga
     3841 tgcgcagggc atacagttgc gactgatccg aggccagcgt atcgcagcga ccagattcca
     3901 gtgccttcgc tgattcgtca gagcgatcga aagtcaccgg tgtgtacttc atattgtttg
     3961 ccttgaaata gtcggcgacg ttgagttcgg tatctgtacc cgcctgaata cagacggtag
     4021 cgccatccag ttctttcgcg ctttttagcc ccgctttatc gtgcgtcagg aagccaatgc
     4081 cgtcgtaatc agtgacgcca gtaaatgcca ttcccatccc ggcatcgcga gatgaagtcc
     4141 aggtcgtatt acgggagagc aaatccacct cccctgactg taaagcggtg aagcgttctt
     4201 ttgcagtgag cggggtatat ttcactttcg tgtcgtcacc aaatacagca gcggcaacac
     4261 cacgacaaac atctacatca atacctgaaa acttaccgtc tgcatcggca taagagaacc
     4321 caggtaatcc atcactgatc ccgcattgca caaaaccttt tttctgtacg gcatcaagcg
     4381 tcgcgccagc atgcgcctga tttgcaacgg caagcagcac gctggcggca gccagtgtgg
     4441 ctatcatcat ctttttcata atgcttcctg tgaggcgaaa attatcgtta taggacgttt
     4501 gggaaacgtc ttttacctgt ctgtggccat cctcttataa gcaaaagtag tgccatcttt
     4561 tgcgcacgat ttcgcgtcgc aataagctga gtgtagacag gaaataataa aaacagcgcc
     4621 ccgcaatggt gcgaaaatga aacatgcgcc acattttggc gcaaaatatt tacgcgtgtt
     4681 ttactaagca gaataatgat tgttagcgtg agtatatttc tggtgggagg tgtgaaagtt
     4741 aaattgatgc gttatttcga aagcagcgaa ataacgcgat ataacatgct aaggtaatga
     4801 ctc
//
LOCUS       DAZPDU010000256         4725 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZPDU010000256 DAZPDU010000000
VERSION     DAZPDU010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4725
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.260"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..738)
                     /locus_tag="ACHURS_004225"
     CDS             complement(<1..738)
                     /locus_tag="ACHURS_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HFR1239810.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAAKAELKLIAEKVKKGYVEQAKDNSLQPSQTVTGSLKVADLSTIIQEQPSFVAET
                     RAPDKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVR
                     EDVMDQIVQEGGLEYATDVIIHLQQIDI"
     gene            complement(879..2912)
                     /gene="metG"
                     /locus_tag="ACHURS_004226"
     CDS             complement(879..2912)
                     /gene="metG"
                     /locus_tag="ACHURS_004226"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFR1239811.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRSDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3044..4153
                     /gene="apbC"
                     /locus_tag="ACHURS_004227"
     CDS             3044..4153
                     /gene="apbC"
                     /locus_tag="ACHURS_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFR1239812.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLNALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            4398..4679
                     /locus_tag="ACHURS_004228"
     CDS             4398..4679
                     /locus_tag="ACHURS_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFR1239813.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQPHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
BASE COUNT         1140 a         1174 c         1185 g         1226 t
ORIGIN      
        1 aatatcaatc tgttgaaggt gtattattac atcagtagca tattccagac cgccttcctg
       61 gacgatctga tccataacat cctcacgcac tttatatctt gtgcatttca tttcaagtgc
      121 cgtgagtaca gccattactg gtgatggtaa ctgtgttgtt ttcaggccgt cgatctgact
      181 gatcgcctgg gcgacggtgt tttgccattc taaagaacag gcgctgaagt caaatgtggc
      241 tgttttatta tcccgttgcg acacactaca ggctaagcga cgtaattggg tcaatttgtc
      301 tgcttgctga acaggaactc cgggaaagcg gcgatgactt aacgtggtgt gtacaacttc
      361 gggcggaaaa acgacagcaa tatctttcgc cagccacggt aaaacagcat ctgtattttt
      421 gtcaggcgca cgggtttctg ctacgaaaga gggttgttcc tgaataatgg tggataagtc
      481 cgctaccttg agagagcccg ttaccgtttg ggaaggttgc aaagaattat ccttcgcttg
      541 ctccacatac cccttcttca ccttctccgc aatcagctta agctccgctt ttgccgctgc
      601 cgcagcatct gaaaaacttt ttatctggct ttgccctttg gtgccaactt ttccccaact
      661 gatatgcaac tcgtttccct gttgctcaac tgcccagaat ttatgtgatt tttcgtcctg
      721 atagataaag tgtctcattc tgcatccctg taattttatc cagttgaatg cagatgctac
      781 cagtatttat gcgggttaga gagagacaaa atcgcaagaa aacaacttta ggaatttata
      841 aagaaaaggc gctgtcgatg cagcgccttg aagggggatt atttcacctg atggcctggt
      901 ttagcaccag catccgggct taacaggaag atatctttcc cgccaggacc agcagccatt
      961 accatgcctt cggagatacc aaagcgcatt ttacgcggtg ccaggttggc caccataatg
     1021 gtgtgacgcc cgatcagtgc ctgcggatcc gggtaggcag aacgaatacc ggagaagaca
     1081 ttgcgttttt caccgccgag atccagtgtc aggcgcagca gtttgtcaga accttcaaca
     1141 aactctgcgt tttcgatcag cgccacgcgc aggtcaactt ttgcgaagtc gtcaaaggtg
     1201 atggtttcct gaatcggatc atctgccagt gggccagtaa ctggcgcggc agctgctttc
     1261 acttcttctt tagacgcttc caccagtgct tcaacctgct tcatatcgat gcggttatac
     1321 agcgctttga acggattcac tttgtggccc agcagcggtt gctggatacc atcccaggtc
     1381 agttctgtat tgaggaatgc ttctgcgcgc tcggtcagtt tcggcagtac cggcttcagg
     1441 taagtcatca gcacgcggaa caggtttatg cccattgagc aaattgcctg caggtcggca
     1501 tcgcggcctt cctgttttgc caccacccac ggagcctgct cgtcgacata gcggttagcc
     1561 agatcagcca gcgccatgat ttcgcgcact gctttaccaa attcgcggct ttcccacgct
     1621 tcaccaatca cttcagcggc atcagtgaag gttttgtaca actgcgggtc agccagttcg
     1681 cttgccagca cgccgtcaaa acgcttgttg ataaagcccg cattacgaga ggccaggtta
     1741 accactttgt tcacgatatc ggcattcaca cgctgaacga aatcctccag gttgagatcg
     1801 atatcatcaa tgcgcgaaga gagtttcgca gtgtagtagt aacgcaggct gtcagcgtca
     1861 aaatgattca gccaggtgct ggctttaata aaggtgccgc gagacttgga catctttgcg
     1921 ccgttcaccg tcacatagcc atgaacaaac aggttggtcg gcttgcggaa gttgctgcct
     1981 tccagcatgg caggccagaa caggctgtgg aagtaaacaa tatctttacc gatgaagtgg
     2041 tacagctcgg cggtggagtc tttcttccag tattcatcga agcttacgct gtcgctgcgc
     2101 ttgtcgcaca gattcttgaa agaacccatg tagccaatcg gtgcgtccag ccagacgtag
     2161 aaatatttgc ccggcgcgtt cggaatttca aaaccaaagt atggcgcgtc gcgggagata
     2221 tcccactgtt gcaggccgga ttcaaaccac tcctgcattt tatttgccac ctgctcctgc
     2281 aacgcaccgc tgcgggtcca tgcctgcaac atttcgctga aagagggcag atcaaagaag
     2341 aagtgttcag aatcacgcat taccggcgta gcgccagaaa ccaccgattt cggctcgatc
     2401 aattcagtcg ggctgtaggt cgcgccgcag acttcgcagt tatcgccgta ttgatccggg
     2461 gatttacatt tcgggcaggt gcctttcaca aaacggtccg gcaggaacat gcctttttcc
     2521 ggatcgtaca gctgagagat ggtgcggttt ttaataaaac cgttctcttt caggcgagag
     2581 tagataagtt ctgacaactg gcggttctct tcgctgtgcg tcgagtgata gttgtcatag
     2641 ctgatgttaa agcctgcgaa atcagtctga tgctcctgac tcatttcgcc aatcatctgc
     2701 tccggggtga taccaagctg ctgagctttc agcatgatcg gtgtaccgtg ggcatcgtcg
     2761 gcgcagatga agttgacctc gtggccgcgc attcgctggt aacggaccca gacatcagcc
     2821 tggatgtgct ccagcatatg gccgaggtgg attgagccgt tagcgtacgg cagtgcgcac
     2881 gtcaccagaa ttttcttcgc gacttgagtc atagtaggca ttacttctta atagtgaaaa
     2941 ggggttagat agtaccaaat gggaaaatgt taagtaagta cttctctata ttcttcaggt
     3001 aaactatcag ctgatggttt atctatacaa aaaaggagtc gggatgaacg aacaatccca
     3061 ggccaaatca ccagaagcct tgcgcgcaat ggtcgccggg acactggcca attttcagca
     3121 cccaaccctg aagcataacc tcaccacgct gaacgcgttg caccatgtcg cctggatgga
     3181 cgacacgttg catgtggaac tggttatgcc attcgtctgg catagcgcgt ttgaagagtt
     3241 aaaagagcaa tgtagcgccg aactgctgcg tatcaccggg gcgaaagcta tcgactggaa
     3301 actgtcgcac aatatcgcca ccttaaaacg cgtgaagaat cagccgggta ttaatggtgt
     3361 gaagaacatc attgcggtca gttccggtaa aggcggcgtg ggtaaatcct ccacggcggt
     3421 aaacctggcg ctggcactgg cggcggaagg ggcgaaagtc ggcattctgg acgccgatat
     3481 ctacggccca tcaattccca ccatgctggg cgcagaaaat cagcgtccaa cttcgccgga
     3541 cggtacccat atggcaccga ttatgtctca tggcctggca accaattcaa ttggttatct
     3601 ggtcactgac gacaatgcga tggtgtggcg tggaccgatg gcgagcaagg cgttaatgca
     3661 gatgttgcag gaaaccttgt ggccggatct cgactatctg gtgcttgata tgccgccagg
     3721 caccggtgac attcaattga cgctggcgca aaacattcca gtaactggtg cagtcgtggt
     3781 caccaccccg caggacatcg ctttgatcga tgcgaaaaaa ggcattgtga tgttcgagaa
     3841 agtcgaggtt ccggtgcttg gtattgtcga gaacatgagt gtgcatattt gcagtaactg
     3901 tggtcaccat gaaccgattt tcggtaccgg tggtgcagag aaactggctg aaaaatacca
     3961 cacacagctg ctggggcaga tgccgttaca catctcctta cgtgaggatc tcgataaagg
     4021 cacgccaacg gttatcagcc gtccggagag cgaatttacc gctatctacc gccagctggc
     4081 tgaccgcgtt gcagctcagc tctactggca gggtgaagtc attccaggcg agatttcctt
     4141 ccgcgcggtc taacgcctat taaccattct ggttattctt catacaccgc agagatgtta
     4201 cattgatgcg gtgtattagt ttttgccgca ttaatgaaca cttatttaat actgcgaata
     4261 ttctgcggac cttcgtccga aactctacaa agtcgattga tcatacaggc aatgcttcat
     4321 tatcatcctt cgtggctgtg tagtggatgt acagctatag tttgttatgc actcattcta
     4381 tccaggcata accttcaatg aataagtatt ggctgtcagg aattattttt cttgcctatg
     4441 gattggcttc tcccgcattc tcttcagaga ctgccacatt agcgattaat ggcaggatca
     4501 gtcccccaac atgcagtatg gctatggtta atggtcaacc tcaaccgcat tgtggtcagt
     4561 taacgtataa tgttgacacc cgtcatctgt tttcttcgcc cgtcaaaggt gtaacgacag
     4621 aagtggttgt tgcaggtagc gatagcaaac gtcgaatcgt gttaaatcgc tacgattaat
     4681 ttggcaacct gattttaatt gcatttagaa agccgaaatc attta
//
LOCUS       DAZPDU010000257         4654 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZPDU010000257 DAZPDU010000000
VERSION     DAZPDU010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.261"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(209..3313)
                     /gene="acrF"
                     /locus_tag="ACHURS_004229"
                     /pseudo
     CDS             complement(209..3313)
                     /gene="acrF"
                     /locus_tag="ACHURS_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
     gene            complement(3325..4482)
                     /gene="acrE"
                     /locus_tag="ACHURS_004230"
     CDS             complement(3325..4482)
                     /gene="acrE"
                     /locus_tag="ACHURS_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFR1239814.1"
                     /translation="MTKHARFFLLPSFILISAALIVGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDECTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
BASE COUNT         1187 a         1201 c         1128 g         1138 t
ORIGIN      
        1 tcttattaac gagctgaatg ccattttatc accggaggtt ataaatattg cggaatgaat
       61 aatgacaaat tatgtgaatt gtaaaaatag tgaagggagc gggaactata gaaagttttt
      121 aaagagtgag tagaagaata tattattaat ttttatgggt ttcactgaaa ataataaagg
      181 cacccgaaag tgcctttatg tttctgattt atcctttaaa gcaacggcgg atcaccacaa
      241 agaacaccgg tacgaagaag attgccagca acgttgcaga gaccattcct cccattaccc
      301 cgatacccac tgcgttctgc gcgccactgc cggcaccgtt actgatagct agcggtaata
      361 cgccgagaat aaaggcgaga gaggtcatca ggataggacg cagacgcata cgtactgcca
      421 tcagtgtcgc ttcaacaaca cctttaccct ttttctccat gagatcttta gcgaactcaa
      481 caatcaaaat agcgtttttg gccgacaagc caattgtcgt tagcaagccc accataaagt
      541 agacgtcatt tttttgatta aagagtgttg ccgccagtag cacgccgaca atccctaacg
      601 gcacgaccaa cataaccgag acaggaattg accagctttc atagagtgca gcaaggcaca
      661 ggaagacaac cacaaaggaa attgctacca gagcgggagc ctggtttccc gataagcgtt
      721 cctgatacga catacctgtc cagtcataac caatgcccgc aggtaatttt gacgcaaggt
      781 tttccatcaa tgccatggca tcgccggaac tggttcctgg cgcggcttcc ccctgaatct
      841 ccattgacgg cagaccgttg tagcgttcca gtcgcggaga gccatacacc caatgtgaag
      901 tggtaaaggc agagaatggc accatttcgc cgttggcgct gcggacataa agtttatcga
      961 catcttctgg cagcatacgg aatttggcat ccgcctgaac atacaccttt ttcacgcgac
     1021 cacggtcgat gaagtcgtta acgtaagtcc cacccagcgc cgttgaaatg gtctgattga
     1081 tgtcagaaag tgaaacacct aatgcctgcg ccttttcctg gtcaacttcc agtttaaact
     1141 gcgcggtgtc ttccagacca ttaggacgca cgctgactaa gctggcagga tgttgcgccg
     1201 ccatgccaag caactggtta cgggcctggg tcagggcatc gtgacccagc ccagcctgat
     1261 caattaactc aaagtcgaaa cccgttgccg tgcccagttc aacaatggct ggcatattga
     1321 atggaatgac aaaaccgtcg cggatcttgc ccaattccat tttggcacga tggattaccg
     1381 cttccgcact gttttcgtca ccactacgct cttcccacgg tttcagactg acgaaagcca
     1441 taccggcgtt ttgtgcctgg ccgctgaagc taaagccgtt aaccgtaaag acactttcta
     1501 cgttcgcttt ctcgttattc agatagtaat ccgtcacttg atccaacact ttttgcgtcc
     1561 gctcttgcgt cgcgccagcg ggtaactgaa tcatggtcag aaagacaccc tgatcctctt
     1621 caggtaagaa ggaagacgga agacgtaaaa acaacaccac cattcctgca acaatcagcg
     1681 catagatcag taaatatcgt cctgtggaac cgaggatttt gccgacgctg ttggtgtagt
     1741 ggttaacgct atgatcgaag gtggtattaa accagccgaa gaaaccgccc ttattttcgt
     1801 gatgctcagc agagacgggt ttaagcagcg ttgcacataa cgcaggggta agaatcaatg
     1861 ccaccagaac agaaagcgcc attgccgaaa cgatggtgat agagaactgg cgataaattg
     1921 ccccagtaga accgccgaag aatgccatcg gaataaatac cgctgacagc accatcgcga
     1981 tacccaccag tgcgccctga atttgcgaca tcgatttttc cgtcgcttct tttggcggga
     2041 gcttatcctc catcatcacg cgctcgacgt tctccaccac cactatcgca tcatcgacga
     2101 gcagcccgat ggcaagcacc atcccgaaca tcgttagtgt gttgatggag taaccaaaag
     2161 cggcgaggat ggcaaacgtc cctaacaaca ccacgggtac cgcaatggtg gggatcagcg
     2221 ttgctcgcat attctgcaag aacagataca tcaccaggaa caccagcata atggcttcga
     2281 acagcgtttt taccacttcg tgaatagaaa gctggacgaa tggcgtggtg tcataagggt
     2341 agagaacctt cattccctgc gggaagaatg gctgtaattc cgccagtttt gccttaatgg
     2401 ctttcgcggt atcgagagcg ttcgcgccgg ttgctagctt aatccccagg cccgccgccg
     2461 gttttccgtt gatacgagcg ataacgttat agttttcacc gccaagttca actcgtgcga
     2521 catctttcag gcgtaccacc gagccgtcac tgtttacgcg cagggttact ttgccgaatt
     2581 cttccggatt ttttaaccgc gtctgagcaa taatcgaggc gttcaactgt tgccctggta
     2641 acgctggcgt tccgcccaac tgcccggcag cgatctgatc gttctgtacc ttcaactggt
     2701 taatcacatc aaccggtgtc agtttatatt tgtttagcag atcggcgtcc agccagatac
     2761 gcatcgcata ctgtgcgccg aaaagctgta cgtcaccgac gccattcaga cggctaagcg
     2821 tatctttaac gttagaggcc acatagtccg agatatcgtc ctgtgtggtg tctgggttat
     2881 cagagacaaa gcccgccacc atcaaatagc tgctactgga cttttcaaca ctgatcccct
     2941 gctgctgaac ctcctgcggc agcaacggcg tggcgagctg gagtttgttc tgcacctgca
     3001 cttgcgcgat atcaggatcg gtcccggact ggaaggtaag ggtaattgtc acgctaccgg
     3061 cggaatcgct ggtggaggac atatacatca ggttatcgat accattcata ttctgttcga
     3121 taacctgcgt caccgtatcc tgcacggtct gcgcatcagc acctggatag tttgcctaaa
     3181 cagaaaccgc aggcggtgca atggttggat actgagcgac gggcaattgt aggatcgcca
     3241 gtgcgcccgc catcatcaga ataatggcca gcacccatgc aaatatcggt cgtcgaataa
     3301 aaaagtttgc catgtcagat taccttactt cgatgcagta tctgcggggg tatcggtagt
     3361 ggctttaacc tgctctcccg gacgcgcttt ttgcaggccg ctgacaatga cttgatcgcc
     3421 agatttcaga ccttcgctaa tcaaccattt atcgccaatc gcctgactgg caacgacagg
     3481 gcgcgcttcc acctgacttt tatcgttaac aatcagcacg gttgcatcac cacgcggtgt
     3541 gcggctaacg ccttgttgcg ggataagaat ggcgtcaggt tggacgcctt catcaatccg
     3601 tgcacgcaca aacatacccg gcaaaagcgt atgttgcggg ttagggaaga cagcacgtag
     3661 ggttatggag ccggtgcatt catcaacggt cacatcagag aattgcagcg tacctttcag
     3721 gggataggtt tgaccgtttt ccatgaccaa ctctacgttg ctggtggcgt tttccttatg
     3781 caaatttcct tgctctacgg attgcttcag cctcataaaa tcgttgctgg attgggtcac
     3841 atcaacgtag ataggatcga gctgctggac agtcgccagt tcagtcgttt gcccattagt
     3901 gacaagagcg ccttcggtca cggtcgattt gccgatacgt ccgctaattg gcgcagtcac
     3961 tttggtataa gcgagattga tgcgagcgct ttcgactgtg gcttttgcgg caatcacggc
     4021 ggcatcggcc tggcgagcat cagcaatggc ctggtcgtac tcctgctggc tgatgtattt
     4081 cgtacccacg agcggaacgt aacgttttac cgtcaaatgc gcgatggcgg cggcggcttc
     4141 acttttcgcc agttcgcctt tcgcgctgtc ataatttgcc tgataggtcg cgggatcgat
     4201 ctggtacagg gactggcctg cttgtacatc gctgccttca gtgaaattac gattcagtac
     4261 gatcccgcta acctgtgggc gaacttcggc tatacggtaa gcattggtgc ggcctggtaa
     4321 ttcagtctta acttctaacg gggccgtttt cacaatatga acggtaacct gcggttcacc
     4381 gacgtgagct ttctcttctc ccttatcgtt acaaccgacg attaaagccg cggagatcag
     4441 aataaaggag ggcaggagga aaaacctggc atgtttcgtc attactattc ctcaaaaaaa
     4501 caaaagcgcg ttatttaccc agaaaggcaa cgcgttaact cgcagaaaga aaaatacagt
     4561 ttgctatcct acaaattatc attcgtcgat gtaaggaata gttatgaata ctggcgtctc
     4621 aaggcacata aacacaaaaa aagattaata ttct
//
LOCUS       DAZPDU010000258         4509 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZPDU010000258 DAZPDU010000000
VERSION     DAZPDU010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4509
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.262"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..461
                     /locus_tag="ACHURS_004231"
     CDS             <1..461
                     /locus_tag="ACHURS_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFR1239815.1"
                     /translation="QNGLAKALREIGRIERTLFMLDWFRDPALRRRVQAGLNKGEARN
                     ALARAVFMHRLGEIRDRKPENQSYRASGLTLLTAAISLWNTVYMERAVDALKRKGMKI
                     NAQLLSHLSPLGWEHINLTGDYIWKNSRIPTSGKFRRLRSVKIDKVKKQP"
     gene            complement(987..2162)
                     /gene="senB"
                     /locus_tag="ACHURS_004232"
     CDS             complement(987..2162)
                     /gene="senB"
                     /locus_tag="ACHURS_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin production-related protein TieB"
                     /protein_id="HFR1239816.1"
                     /translation="MNIFTLSKAPLYLLISLFLPTMAMAIDPPERELSRFALKTNYLQ
                     SPDEGVYELAFDNASKKVFAAVTDRVNREANKGYLYSFNSDSLKVENKYTMPYRAFSL
                     AINQDKHQLYIGHTQSASLRISMFDTPTGKLVRTSDRLSFKAANAADSRFEHFRHMVY
                     SQDSDTLFVSYSNMLKTAEGMKPLHKLLMLDGTTLALKGEVKDAYKGTAYGLTMDEKT
                     QKIYVGGRDYINEIDAKNQTLLRTIPLKDPRPQITSVQNLAVDSASDRAFVVVFDHDD
                     RSGTKDGLYIFDLRDGKQLGYVHTGAGANAVKYNPKYNELYVTNFTSGTISVVDATKY
                     SITREFNMPVYPNQMVLSDDMDTLYIGIKEGFNRDWDPDVFVEGAKERILSIDLKKS"
     gene            complement(2231..4492)
                     /locus_tag="ACHURS_004233"
     CDS             complement(2231..4492)
                     /locus_tag="ACHURS_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFR1239817.1"
                     /translation="MNVIKLAIGSGILLLSCGAYSQSISEKTNSDKKGAAEFSPLSVS
                     VGKTTSEQEALEKTGATSSRTTDKNLQSLDATVRSMPGTYTQIDPGQGAISVNIRGMS
                     GFGRVNTMVDGITQSFYGTSTSGTTTHGSTNNMAGVLIDPNLLVAVDVTRGDSSGSEG
                     INALAGSANMRTIGVDDVIFNGNTYGLRSRFSVGSNGLGRSGMIALGGKSDAFTDTGS
                     IGVMAAVSGSSVYSNFSNGSGINSKEFGYDKYMKQNPKSQLYKMDIRPDEFNSFELSA
                     RTYENKFTRRDITSDDYYIKYHYTPFSELIDFNVTASTSRGNQKYRDGSLYTFYKTSA
                     QNRSDALDINNTSRFTVADNDLEFMLGSKLMRTRYDRTIHSAAGDPKANQESIENNPF
                     APSGQQDISALYTGLKVTRGIWEADFNLNYTRNRITGYKPACDSRVICVPQGSYDIDD
                     KEGGFNPSVQLSAQVTPWLQPFIGYSKSMRAPNIQEMFFSNSGGASMNPFLKPERAET
                     WQAGFNIDTRDLLVEQDALRFKALAYRSRIQNYIYSESYLVCSGGRKCSMPEVIGNDW
                     EGISDEYSDNMYIYVNSASDVIAKGFELEMDYDAGFAFGRLSFSQQQTDQPTSIASTY
                     FGAGDMTELPRKYMTLDTGVRFFDNALTLGTIIKYTGKARRLSPDFEQDEHTGAIIKQ
                     DLPQIPTIIDLYGTYEYNRNLTLKLSVQNLMNRDYSEALNKLNMMPGLGDETHPANSA
                     RGRTWIFGGDIRF"
BASE COUNT         1146 a         1052 c         1049 g         1262 t
ORIGIN      
        1 aacaaaatgg gttggctaaa gctctgagag agattggccg tatagaacga acactgttta
       61 tgctggactg gtttcgggat ccggcattgc ggcgacgtgt ccaggccggg ctgaacaaag
      121 gagaagcccg taatgctctg gcccgtgctg tattcatgca tcgattgggg gagattagag
      181 atcggaaacc ggaaaatcaa agttatcgcg ccagtggatt gactttattg acggcggcta
      241 tttccttatg gaacacagtt tacatggaaa gagcggttga tgctctgaag cgtaagggca
      301 tgaaaataaa tgctcaacta ttatcgcatt tatcaccatt aggttgggaa catatcaatc
      361 tgacaggcga ttatatctgg aaaaacagcc ggataccgac ttcaggtaag tttcgtcgtt
      421 tgagatcagt taagatcgac aaggtcaaaa aacaacctta acgtacaata attttcgata
      481 tccaaactga ccccaagtat aaaaaacagc ctgtcacggt ataaaaatcg accatgcaga
      541 aattatgctg gttattttac cgctgctggt ccctcattca gtgactgccc caggggtttt
      601 catgctctcc ttatagtaca ataatttcca tatccaaact cacccctact ttgactttta
      661 tcatagtcaa aacctcttaa cgtgagtttt cgttccactg agcgtcagcc ctctaaaact
      721 actgaataat cagttagaaa acaaacaatc ggaaaaatct ggggttccgg cctagaaccc
      781 catgaacagg catagctgtg atttcattta attattggca caaatcagtt gatcgtacaa
      841 tgctgtgcct gtctcgtttt ctggagattc ctcagcctca tggagcggcg taacaggcgt
      901 accggcagga cagagatgtt tgcttttcct agtccggcca gtttagtgga ataacggagt
      961 ttttatccca tgttttttcc ccccgatcac gactttttca aatcaatgct cagaatacgt
     1021 tctttagctc cttccacaaa cacatcagga tcccaatcgc ggttaaagcc ttctttgatg
     1081 ccaatgtaaa gggtatccat atcgtccgac aacaccatct ggtttgggta gaccggcatg
     1141 ttaaattcac gggtgatgct gtatttggtg gcatccacta cgctgatggt gccgctagtg
     1201 aagttggtga catacagttc gttatatttc ggattgtatt tcaccgcgtt agctccggct
     1261 cctgtgtgca catagccaag ctgtttaccg tcgcgtaagt caaaaatata gagtccatct
     1321 tttgtaccgg aacgatcgtc atggtcgaat accaccacaa aggcacggtc agaagcggag
     1381 tccaccgcca gattctgcac acttgtgatt tgtggtctcg gatctttcaa cgggatggta
     1441 cgcagcagcg tctgattttt cgcatcaatt tcgttgatgt aatctcttcc gccaacgtag
     1501 attttctgtg ttttttcatc catcgtcaga ccatacgctg tacctttgta agcatcctta
     1561 acctcgcctt ttaaggcaag cgtcgtcccg tcgagcatta acagcttatg cagaggcttc
     1621 atgccctcgg ccgttttcag catattgcta taactcacaa acagggtatc ggaatcctgg
     1681 ctgtaaacca tatggcgaaa atgctcaaaa cgcgaatctg cagcgtttgc cgctttaaaa
     1741 cttaacctgt cgctggttct taccagtttg ccggttgggg tgtcaaacat actgatacgc
     1801 agggacgctg actgggtgtg tccgatatag agctgatgtt tatcctgatt tatcgccagc
     1861 gaaaatgccc ggtatggcat cgtgtattta ttttcgactt tcagcgaatc tgaattaaac
     1921 gaatacagat agcctttatt ggcttcacga tttacacgat cggtgactgc tgcaaacacc
     1981 tttttactgg cattatcaaa cgccagttca tagacgcctt catcagggga ctgaaggtaa
     2041 ttcgttttca gggcaaatcg cgaaagttcg cgttcaggtg gatcgatagc catggccatc
     2101 gtgggtaaaa atagtgaaat taacaggtat agcggtgctt tggataaagt gaaaatattc
     2161 atttttcgtt cctgcaataa aattgacata agagagaaaa cgtattgtct gaacctagcg
     2221 gaaattgtca tcagaaacga atgtccccgc caaatatcca tgttctgcca cgcgcggaat
     2281 tggctgggtg ggtctcgtca ccaagacctg gcatcatgtt gagcttattc agcgcctccg
     2341 aataatctct gttcatcagg ttttgtaccg aaagtttcag tgttaggttg cggttgtact
     2401 cgtaagtacc atagagatcg ataatcgttg ggatctgcgg caaatcctgt ttgattattg
     2461 cgccggtatg ttcgtcctgc tcaaaatcag gcgacaggcg acgagccttg cctgtgtatt
     2521 ttatgatagt gcccagggtc aacgcgttat cgaagaagcg aacaccagta tccagcgtca
     2581 tgtattttct gggcagttcg gtcatatccc ctgcgccaaa gtaggtgctg gcgatggagg
     2641 ttggctggtc tgtttgctgc tggctgaaag agagtcggcc aaaagcaaaa cctgcatcat
     2701 aatccatctc cagttcgaag ccctttgcga taacgtcgct tgccgagtta acgtagatgt
     2761 acatattgtc gctgtattca tcgctaatgc cctcccagtc attgccaatc acctcaggca
     2821 tactgcattt acgacctcca gaacaaacca gataagactc gctgtagatg tagttctgga
     2881 tcctgctgcg gtacgccaga gccttaaagc gcagggcatc ctgttcgacc agtaaatctc
     2941 tggtatcaat gttaaaaccc gcctgccagg tttctgcacg ttcaggcttc aggaatgggt
     3001 tcatggatgc gcctcctgaa ttagagaaga acatctcctg gatgttcggg gcgcgcatgg
     3061 atttgctgta gccaatgaac ggctgaagcc atggtgttac ctgagcagaa agctgaactg
     3121 aagggttgaa gccaccctct ttatcgtcaa tatcgtagct accctgtggc acgcagataa
     3181 cgcgtgaatc gcaggcgggc ttgtaccctg tgatcctgtt acgtgtgtag ttgagattga
     3241 aatctgcctc ccagatgccg cgcgtaacct tcagcccggt atacagcgct gaaatatcct
     3301 gctggccgga gggtgcgaac ggattgttct cgatcgattc ctgattcgct ttcgggtcgc
     3361 ccgccgctga gtgaatggtc cggtcatagc gggtacgcat cagtttgctg cccagcataa
     3421 actccaggtc attgtccgcg acagtgaacc ggctggtatt gttgatatcc agcgcgtcag
     3481 aacgattttg cgctgaggtt ttgtagaaag tatacagcga gccatcacga tacttttgat
     3541 taccgcgact ggtactggcc gttacgttaa agtcaattaa ttcagaaaaa ggggtgtaat
     3601 gatatttaat gtaatagtcg tcactggtta tatcacgacg tgtaaattta ttttcatagg
     3661 ttcgagcgga aagttcgaag ctgttaaatt cgtctggtct gatatccatt ttatacagtt
     3721 gggatttggg gttctgcttc atatatttat cataaccaaa ctctttgctg ttaattccag
     3781 aaccatttga gaaattagag tacacagaac tgccgctcac agcagccata acgccaatgc
     3841 ttcccgtatc cgtaaaagcg tcgctttttc caccaagggc gatcattccg ctgcgtccca
     3901 gcccattact accgacagag aaacgtgaac gaaggccata tgtattaccg ttaaatatta
     3961 catcgtcaac gccaatagta cgcatatttg cactaccggc aagggcgttg atcccttcag
     4021 agccactgct gtcaccgcgt gtaacatcaa ctgctaccag taagttagga tctataagta
     4081 cgccagccat attgttagtt gaaccatgcg tcgttgttcc ggaggtagag gttccgtaaa
     4141 aactctgggt aataccatcg accatagtgt ttacacgacc aaatccgctc atgcctcgaa
     4201 tattcacact gattgctccc tgaccaggat ctatttgagt ataagtacca ggcatactac
     4261 gcactgttgc gtcaagtgat tgcaggtttt tgtccgttgt ccgggaactg gtcgcgcctg
     4321 ttttctcgag agcttcctgc tcactggtcg tcttcccgac agaaacgctg agcggactga
     4381 attctgccgc tccttttttg tcggaattag ttttttcact gatggattgt gagtaagcac
     4441 cgcagctgag caataatatg cctgagccga tagccagttt tataacgttc ataaatttcc
     4501 ttgggtata
//
LOCUS       DAZPDU010000259         4481 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZPDU010000259 DAZPDU010000000
VERSION     DAZPDU010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4481
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.263"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(8..1069)
                     /locus_tag="ACHURS_004234"
     CDS             complement(8..1069)
                     /locus_tag="ACHURS_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFR1239818.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWQCLWCLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(1066..2463)
                     /locus_tag="ACHURS_004235"
     CDS             complement(1066..2463)
                     /locus_tag="ACHURS_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFR1239819.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDTWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTVDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            2618..3616
                     /locus_tag="ACHURS_004236"
     CDS             2618..3616
                     /locus_tag="ACHURS_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR1239820.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(3727..>4481)
                     /gene="ompG"
                     /locus_tag="ACHURS_004237"
     CDS             complement(3727..>4481)
                     /gene="ompG"
                     /locus_tag="ACHURS_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFR1239821.1"
                     /translation="EPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVH
                     YQFLENDDFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWL
                     SMYKFANDLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFST
                     QEIRAYLPLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGL
                     SVSLEYAFEWQDHDEGDSDKFHYAGVGVNYSF"
BASE COUNT         1092 a         1227 c         1099 g         1063 t
ORIGIN      
        1 tattgcatta tttttcgctg ggtgttttgc ctttttgcag tgtttctttc acctgaccga
       61 taagctgacg acggaaatcc ccgaggcgag gtttgtcatc gtcaatccac ggcagcgggc
      121 ggcaaagctc catagcttta atcccgagtc gtgccgtcag aagtcctgca ccaatcccct
      181 gagctgcgcg ggtagacaaa cgagcagcga gatcttgcga catccagtcc atccccactt
      241 cgcgcaccag ttcgctggct ccggcaaaag cgatattcag caataccagc ttaaacagac
      301 gcaaacggct gtaatacccc agttcaatgc catacagcgt ggcgatgcga ttaattaaac
      361 gcagattgcg ccaggcgata aacgccatat cgaccaacgc cagcgggctg accgcaatca
      421 tcaacgttga ttccgccgcc gaacggctga tttcgcgccg cgcctgggca tctaaaactg
      481 gctggaccaa atgcgcatac aggctgacca cttcacggtc gttttgcgtt tcatggattg
      541 aggcatacca gcgttgcagc gccggatgcg actgatcaat acccgcctgc tgcgccagtt
      601 tttcgcaaaa tgcgcggcct ttgcccgtgc cgtggctatg caataaatca cgcgcttcgt
      661 cgcgttcatg ggcgcgctgt cgcaagcacc ataagcactg ccactctgtt accacagaac
      721 ctacgccagc gccgataatc aatgccccag cggcacatcc acccagcgct acccagtcct
      781 gggtttgcca ggcattcatt gtccactgta caccctgccc gacaacactt gcgccaaaca
      841 gagccagccc gcccatcacc attttgcgcc acaggctgcg tttcggacgt aacgctgcat
      901 ccattaccgc ttcaacttgc ccctcttctt caggcgcttc atcgagcgtg gccggggcaa
      961 aattttgcgc ctgattttcg tcaaaggtct gctgcgccct gaattttgga ttctgatcga
     1021 cctccagcgg accgtcgaaa tcaatacgtg gttttaacgg ttcggtcatc gcaatttatc
     1081 tcctattaaa aattccagcg cggcatcaag acgaatatgc ggcagcggtt tgtcgacatc
     1141 catcacctgc gggcgaaacg cttcaaactg gaacccttgc ttgtcccaga acgcctgacc
     1201 gggcaaacgc gcaggaactt caccggggta aacggtgaga ggcgcaccat cgctgagccg
     1261 attaccgcgc aatgccggaa ttttctcgcc gttgacatca ataatgccgc tggtggtcgc
     1321 ctgcaccgag gccagcccca ggcaatccat actaatccct tcaaacgccg cattttgcca
     1381 ggcatcctga atcagttgtt gcagtaatga aaccatattg gcgtgctgat cgaccgttac
     1441 atggtccgct ttcgttgcgg caaacagcag tttgtcgata accggcgaga ataagcggcg
     1501 aaagagcgtg cgctgcccgt agtggaaact ttgcatcagc tgcgtcagtg ccagacgcat
     1561 atcattaaat gcctgtggcc cactgttgag aggttgcagg caatccacca gcacaatctg
     1621 gcggtcaaag cgcagaaaat gattcttata gaaccccttc accaccttct cgcagtaata
     1681 attaaaccgc tcgcgcagca ttccggcatt ggtgtgctta tcggcctgcg ccagtttgga
     1741 ctcgccccag gtatcgacat ccggccacgg gaagaattgc agcgcgggcg cacctgccat
     1801 atctcctggc aagacaaagc gccctggctg aataaagtgc agcccctgct gtttacagtg
     1861 gtggagataa tcggtccacg cggcggcaat gtctgccagc cggttttcgt cggcaggtgc
     1921 tagcgggtcc agcccttcgc acatcattcg ccatttgacc gaccattcgc cgcgctgacc
     1981 attgagtaag cccgtcatct ggcgtgacca gcttaaatag tcctgcgcca gcatcggcag
     2041 gtcgagcaac cattcgccgg gataatccac aatctccaga tacagcgtgg aggtgtcttt
     2101 gaagtggcgt agcagcgaat cgttcgattt aaaacgtagc gccaggcgga tttcactgac
     2161 gccgcgcgta ggcgtcggcc aggcgggagg atcgccatat aactgcgcca gtccttcatc
     2221 ataggtgaag cgcggaatgc caaagtcacg ctgaggaatg cgttttacgc ccagcaggcg
     2281 ctcttcacgc accgcgctta acagcggcaa gcgtgctccg gcgtgaatat tgagcaactg
     2341 attgaccatc gcagtgataa acgctgtttt gccgctgcgg cttagtccgg ttacggcgag
     2401 gcgcagatgt ctgtcgacac cccgattcac cagcgcatta agttcatttt taagtcgctt
     2461 cattgctgtc ctgtgctgct ctgaagacca gaaaaaaatc attcgtaagg atatgaatgt
     2521 gggggccagg ccattgatta tcaactgccc gtgttgtcat cgacgggcga actccataag
     2581 atgtgcagca ttcacgttcg gacggagttc ctgacctatg tcccctacta tttatgatat
     2641 tgccagggtt gcaggcgtat caaaatccac cgtatcacgc gtgctgaata agcaaactaa
     2701 tatctccccg gaagcgcgcg aaaaagtgtt acgggccatt gaagaattac agtatcagcc
     2761 aaacaagcta gcccgcgcgc tgacctcttc gggttttgat gccattatgg tgatttctac
     2821 ccgttcgacc aaaactacgg cgggtaatcc gtttttctct gaagttttgc atgccatcac
     2881 cgccaaagct gaagaagaag gtttcgacgt gatattgcag acgtcgcaca acccggcaga
     2941 agacttacaa aaatgtgaaa gcaaaattaa gcagaaaatg attaaaggca ttattatgct
     3001 aagttcgcca gcggatgagt cattttttgc ccaactcgat aaatacgata ttccggtggt
     3061 ggtgattggc aaagttgaag gtcaatatgc ccatgtttat tctgtcgata ccgataattt
     3121 tggcgacagc attgcgttga ccgatgcatt aattgaaagt gggcatcaaa atattgcctg
     3181 cctgcatgct ccgcttgatg tccatgtttc agtggatcgg gtaaatggtt ataagcagag
     3241 cctggctgcg cataatattg cagtgcgtga tgaatggatt gttgacggtg gttataccca
     3301 tgaaacagca ctgcaagccg cgagagaatt attaagccag tcaccgttgc ctgaggcggt
     3361 gtttgccact gacagtctga aattaatgag tatttatcgt gcggcagcag agaaaaatat
     3421 tgctattccg cagcagttag cggtggtggg ttatagtaat gaaacgctgt cgtttatatt
     3481 aacgcctgca ccgggcggca tcgatgttcc gacgcaggag ttaggtcaac aaagctgcga
     3541 gttattattc cgcttaattt ctggaaaacc gtcaccacag aatattaccg ttgccacgca
     3601 tatgacgctg aaataaatac cagctacctt gcctgaatgc cggatgcgat gctagcgcat
     3661 cttatccggc ctacatttat agatcggata aagtactcgc gccgcatccg gcaatttagc
     3721 ccattatcag aacgagtaat ttacgccgac acctgcataa tggaatttat cactgtctcc
     3781 ttcgtcgtga tcctgccact caaacgcgta ttccagcgaa acggaaagtc cgttctggaa
     3841 atcataaccg taaaataaac ctacacggtt aaaatcatgg ccttcacgtt caatatcatc
     3901 ctgccagtcc cagttactcc agcgatccag cccaatgcgc gtatacggcg tcaccgagtg
     3961 gttgccgagc gtcagcggca aataggcgcg aatttcttgc gtggaaaact caccgttatt
     4021 gcggctgtcg tccatattga agccgcgctc gagataatag ttcactcgca aggcaaccgt
     4081 ttcgttgaag gtatattgca gacctgtttc cgtttcgaca cgggtatcag cgtaaccggt
     4141 agtgttcaga tcgttggcaa atttatacat cgacaaccaa ccgttgaaac gtaaatcgtc
     4201 agtcagtttc acatcccagt ctggcgcgat tttccagcgc tgcatattcg ccgtgtcttt
     4261 acccggttca tcaacgtagt gataaccata attacggaaa ccgccggtca ggccgaaact
     4321 gaaatcatcg ttttcgagga actgataatg cacctccagc tccgggcgat caaaccacgt
     4381 tccacgttta cccgcgctat aatctaccgg cccttcctga taataggcca gagcaattct
     4441 ccacggcccg ttggcggcat taaaatagac tgaaggctcc g
//
LOCUS       DAZPDU010000260         4478 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZPDU010000260 DAZPDU010000000
VERSION     DAZPDU010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4478
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.264"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(1..1133)
                     /locus_tag="ACHURS_004238"
                     /pseudo
     CDS             complement(1..1133)
                     /locus_tag="ACHURS_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(1133..1315)
                     /gene="ymfR"
                     /locus_tag="ACHURS_004239"
     CDS             complement(1133..1315)
                     /gene="ymfR"
                     /locus_tag="ACHURS_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfR"
                     /protein_id="HFR1239822.1"
                     /translation="MIMLILAPLVGVLGALLLAYGAWLIYPPAGFVVAGALCLFWSWL
                     VARYLDRTRQSVGGGK"
     gene            complement(1327..3060)
                     /locus_tag="ACHURS_004240"
     CDS             complement(1327..3060)
                     /locus_tag="ACHURS_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFR1239823.1"
                     /translation="MSRKSYPNVNAANQYARDVVRGKIVACQFVIQACQRHLDDLMAE
                     KSKSFRYRFDKDLAERAAKFIQLLPHTKGEWAFKRMPITLEPWQLFVICCAFGWVNKG
                     TRLRRFREVYTEIPRKNGKSAISAGVALYCFACDNEFGAEVYSGATTEKQAWEVFRPA
                     RLMCKRTPMLTEAFGIEVNASNMNRPEDGARFEPLIGNPGDGSSPHCAVVDEYHEHAT
                     DALYTTMLTGMGARRQPLMWAITTAGYNIEGPCYDKRREVIEMLNGSVPNDELFGIIY
                     TVDEGDDWTGPQVLEKANPNIGVSVYREFLLSQQQRAKNNARLANVFKTKHLNIWVSA
                     RSAYFNLVSWQSCEDKSLTLEQFEGQPCILAFDLARKLDMNSMARLYTREIDGKTHYY
                     SVAPRFWVPYDTVYSVEKNEDRRTAERFQKWVEMGVLTVTDGAEVDYRYILEEAKAAN
                     KISPVSESPIDPFGATGLSHDLADEDLNPVTIVQNFTNMSDPMKELEAAIESGRFHHD
                     GNPIMTWCIGNVVGKNMPGNDDVVKPVKEQAENKIDGAVALIMAVGRAMLYEKEDTLS
                     DHIESYGIRSL"
     gene            complement(3057..3551)
                     /locus_tag="ACHURS_004241"
     CDS             complement(3057..3551)
                     /locus_tag="ACHURS_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFR1239824.1"
                     /translation="MAGTAGRSGRRPKPTARKALAGNPGKRALNKDEPVFTPIKGVEP
                     PEWFAEEDLPLATIMWQLTTKELCGQGLLCVTDLAVLERWCVAYEFWRRAVKNIAIQG
                     NTITGAMGGRVKNPELTAKKEQESEMSSTGAMLGLDPSSRQRLIGLAGQKKATNPFLK
                     IIES"
     gene            complement(3673..4023)
                     /locus_tag="ACHURS_004242"
     CDS             complement(3673..4023)
                     /locus_tag="ACHURS_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR1239825.1"
                     /translation="MPSLIPRACRKRGCAGTTTDSSGYCDKHRGEGWVQHQRGLSRHQ
                     RGYGSKWTVIRARILKRDKGLCQLCLRVGVVSEAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERIR"
     gene            complement(4066..4476)
                     /locus_tag="ACHURS_004243"
     CDS             complement(4066..4476)
                     /locus_tag="ACHURS_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR1239826.1"
                     /translation="MSWAVNHYRDNAITYKEQRDKNARELKLANATITDMQQRQRDVN
                     ALDAKYTKELADAKAENETLRADVAAGRRRLHIKAVCQSVREATTASGVDNATSPRLA
                     DTAERDYFTLRERLMTMQMQLEGAQEYIRTQCIK"
BASE COUNT          962 a         1326 c         1127 g         1063 t
ORIGIN      
        1 gggacgcgga ttcatatctt ccaggtcgcg gcagtcattg gaagagtaaa ttccccagtt
       61 gatcccggtg gcgtaggctt caaaacggga cttcatatcc ccgcgcagta acgccccggc
      121 gttaaatttg gcgtaataaa cgccctgctt actttttcgt accagtccgg tgttgatccg
      181 ctgttcgatg cgggtcagat acggcaccag tgaatagttg ataaatccca gccccagctc
      241 ttcgatattg ttgaaggtgg cgcgatcggt gttctgcacc atgtgcaacg gcacccggaa
      301 cagacgacag atttcttcca gctgaaactt gcgggtttcc aggaactgac tgtcctcggc
      361 gttcagcgcc atcgacttcc agtccagccc catttccaga atcatcgggc ggtgagcatt
      421 gccaagcccc gtgtgacact cctcaaaatc tttcttcaga cgctcgtaag catccggcgt
      481 gagcttttgt tccgtacgca acacaccgga tgtcaccgca ccattactaa acaacctggc
      541 accgtgctcc tcggttgccg ctgccagtga aatggcctca cgcgcatacg caatgggatt
      601 cagcccgaca agtccatcca gcgtcagggt gcgcacatgc cagatttcat tctgggtcaa
      661 cacatccacg gaaccatccg gaaacgtcac ctgataaacc ggctgccact ggctgttcag
      721 cttcggttcc acacagcccg gatctatcgg aagaagctcc accacttctc ccagtgcctt
      781 taccttgtag gcgtaaaaat tcccccgtag acacaggcag acaataacca gctcccagaa
      841 ttcctgcggt gtcatgtagc cattaggttt tgccgaaacc aacttatgca gtcgttcatc
      901 caccgcccgt gttttcaggg taccgctgat tttgtagaga ctgcagggca gcataccaac
      961 agactctgcc agcaccctga cgcaagaata gaccgccgtc aggcgcatgg cccgctggct
     1021 actgatccgc tttccggtat aggtgtcgta tgacagcccg atagcatcag cagttctgct
     1081 ggcgtggtca ccggcacatc gctttttcgt tgaaataatc ccgaaaagaa cactatttac
     1141 ctccgccgac agactgccgt gtacggtcga gatatcgcgc caccagccac gaccagaaca
     1201 ggcacagcgc cccggcaaca acaaaacccg ccgggggata aatcagccag gcaccatacg
     1261 ccagcaaaag cgcacccagc acgcccacca gaggcgcgag aatcagcatg atcataatta
     1321 cctcagttaa agcgagcgga tcccgtagga ctcaatgtgg tcagacagcg tgtcttcttt
     1381 ctcgtacagc atggctctgc caaccgccat aatcagcgca actgcaccat cgattttgtt
     1441 ttccgcctgc tctttgacgg gtttcaccac atcatcgtta cccggcatgt ttttgccgac
     1501 cacgttgccg atacaccagg tcatgatggg attgccgtca tgatgaaaac gtcccgattc
     1561 aatcgctgct tccagctctt tcatcggatc ggacatattg gtgaagttct ggacgatagt
     1621 gacgggattc aggtcttcat cagcaaggtc atgtgacaac ccggtcgccc cgaaggggtc
     1681 gatgggtgac tcactgaccg ggctgatttt gttcgccgct ttggcctcct cgaggatgta
     1741 gcgataatcc acctccgcac catcggtaac ggtcagaacg cccatttcca cccatttctg
     1801 aaagcgttcg gctgtccgtc gatcttcatt tttctcgacg ctgtacaccg tgtcatacgg
     1861 tacccagaaa cgcggggcca cactgtagta atgcgtttta ccgtcaatct cgcgggtata
     1921 aagtcgcgcc atgctgttca tatccagctt acgcgccagg tcaaaggcca gaatgcacgg
     1981 ctgcccctcg aactgctcaa gggtcagtga tttatcctcg cagctctgcc agctcaccaa
     2041 gttgaaatac gccgaacgcg ccgacaccca gatattgagg tgttttgttt taaagacgtt
     2101 tgccagacgg gcgttatttt tcgcacgctg ctgctgactt aacaaaaatt cgcgataaac
     2161 cgacacgcca atatttggat tggctttttc cagcacctgc gggccggtcc agtcgtcacc
     2221 ttcatcaacg gtatagatga tcccgaacag ttcatcgtta ggcaccgagc cgttgagcat
     2281 ctcgatgact tcccgccgct tgtcgtagca cggcccctca atgttgtacc cggcggtggt
     2341 gatggcccac atcagtggct gacgtcgcgc ccccatcccg gtaagcattg tggtataaag
     2401 cgcatcggtg gcatgctcgt gatattcatc aaccacggca cagtggggtg atgaaccatc
     2461 acctgggttg ccgatcagcg gttcaaaccg cgcgccatcc tccggacggt tcatgtttga
     2521 ggcgttaacc tcaatcccga acgcttccgt cagcatgggt gtgcgtttac acatcagtcg
     2581 cgccgggcga aagacttccc acgcctgttt ctctgtcgtg gcaccggaat acacttccgc
     2641 gccaaactcg ttatcacagg caaaacaata cagggcaaca ccggcagaga ttgctgattt
     2701 gccgttctta cgggggattt cggtgtacac ctcccggaag cggcgcaacc gggtgccttt
     2761 attgacccag ccaaacgcac agcagatcac aaatagctgc cacggctcca gcgtgatggg
     2821 catccgtttg aatgcccact cccccttggt gtgcggcaac agctgaataa atttcgcggc
     2881 ccgttcagcc aggtccttgt cgaagcggta acgaaacgac ttactttttt ccgccatcag
     2941 gtcatcaaga tggcgctggc aggcctgaat cacaaactgg caggcaacaa tctttccgcg
     3001 cacgacatcc cgggcatact gatttgcagc atttacgttg gggtaagatt tccggctcat
     3061 gattcgataa ttttcagaaa cgggttagtg gctttcttct gccccgccag gccaatcaga
     3121 cgctggcggc tgctggggtc gagtccgagc attgcccccg tgctgctcat ctcggactcc
     3181 tgttcttttt tggcggtcag ctccggattt ttgaccctgc cgcccattgc accggtgatg
     3241 gtgttgccct gtatggcaat atttttcacg gcacgtcgcc agaactcata ggccacgcac
     3301 caccgctcaa gtaccgccag gtcagtcacg cacagcaggc cctgaccgca gagttctttg
     3361 gttgtcagtt gccacatgat cgtggcgaga gggagatctt cttcagcgaa ccactctggt
     3421 ggctcaacac ctttgatggg cgtaaaaaca ggttcatctt tgttcagggc tcgcttgccg
     3481 gggtttccgg ccagcgcctt gcgcgccgtt ggcttggggc gacgcccgga acgccccgcc
     3541 gttccagcca tatgcggcac tcctggttaa atttcatttt tcgcgggtat aaaaaaacga
     3601 tggggcgggc agtccggaag acgtcaggtc acagggattt gccccgcccc tccctacaag
     3661 tgagaataat tatcaccgga ttcgttcgcg cgctgttttc gctttatggc agggccagca
     3721 cagactctgc aggttgctgt ctgcgtctgt tccgccatgc gctttcggga tgatgtggtc
     3781 gacggttttc gcctcgctca ccacaccgac acgcagacac aactgacaca gacctttatc
     3841 gcgcttcaga atacgggcac gaatcaccgt ccattttgag ccatagccac gctggtggcg
     3901 gctcagtccg cgctggtgct gcacccagcc ttcaccgcga tgtttatcgc agtaacccga
     3961 actgtctgtg gttgtacctg cacatccacg cttacggcag gcacgtggga ttagtgatgg
     4021 cataaatacc tcataccctg cgaaatgttt accacgataa aaaggctact taatgcactg
     4081 agtgcggata tactcctgtg ccccttccag ttgcatctgc atcgtcatca gccgctctct
     4141 gagggtgaaa taatcccgtt cagcggtgtc tgccagtcgg gggctggttg cattatccac
     4201 gccggaggcg gtggtggctt cacgcactga ctgacagact gctttgatgt gcaaccgacg
     4261 acgaccagcg gcaacatcag cgcgcagagt ttcattttca gctttcgcat cagctaactc
     4321 cttcgtgtat ttagcatcga gtgcattaac atcacgctgt ctctgctgca tgtcagtaat
     4381 ggttgcgttc gccagcttca gttctcttgc gtttttgtcg cgctgctctt tgtaggtaat
     4441 ggcgttatca cggtaatgat taacagccca tgacaggc
//
LOCUS       DAZPDU010000261         4409 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZPDU010000261 DAZPDU010000000
VERSION     DAZPDU010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.265"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..105)
                     /locus_tag="ACHURS_004244"
     CDS             complement(<1..105)
                     /locus_tag="ACHURS_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239827.1"
                     /translation="MIKPFLMTVAILTSCCAQAKILTNEEIVSRFISNV"
     gene            complement(173..2122)
                     /locus_tag="ACHURS_004245"
     CDS             complement(173..2122)
                     /locus_tag="ACHURS_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR1239828.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLLLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKAHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2207..2542)
                     /locus_tag="ACHURS_004246"
     CDS             complement(2207..2542)
                     /locus_tag="ACHURS_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFR1239829.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPRSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(2542..2898)
                     /locus_tag="ACHURS_004247"
     CDS             complement(2542..2898)
                     /locus_tag="ACHURS_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFR1239830.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(2898..4394)
                     /locus_tag="ACHURS_004248"
     CDS             complement(2898..4394)
                     /locus_tag="ACHURS_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFR1239831.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
BASE COUNT         1021 a         1313 c          970 g         1105 t
ORIGIN      
        1 aacgttacta ataaatcgtg aaacaatctc ctcatttgtt agtattttcg cctgagcaca
       61 gcaagaggtt aatattgcaa ccgtcattaa aaatggcttt atcacaaaac aaactccatt
      121 gttagcatgt tacgcgataa ttttgcctca tctttcacca caaaaaaaga actcaccttt
      181 taaatggaga atacccaaca tcatgggtga ttttcataaa gggatcgcct gttttcggca
      241 aatcgataac gcgcattcca ggcggcgcgt tgtcaaacgt caccttaagt tcgctgcggg
      301 ttgccggtga agatgcaagg cttaacagct gattcctttc cagtgataca ttcggggtgt
      361 agcgtttatc gggagataaa tacccatcac cacgtggcat ctgccatccg gtcatgtcat
      421 agacaaaatc atggaactgt ttgccccact cgtcgaactt atcattcaga ccaaatccac
      481 tgttcagtgc atcagccaca aaatttttaa caagccaggg atgtgctttt tcaaactcct
      541 gcgccatcat gcccagttca agcaatccac caatcagact gatctttccg aggcccttta
      601 atttcagccc tgtttttcct gccgccgttg tccattgatt ctgggcgact gttgccgcac
      661 gaatacttgc caccgccttc acaccaatat acagggcaga aaccgttgct attgttttta
      721 ctgcctgttc ccatcccccc attgctctga caacctgatc aacctcctgc cagacttttt
      781 tcacaaccgg accgaccgtt tcccagttat caattatgag atatgcacca ccgacgagaa
      841 gcgcaattaa tccctttgcc ggcgtcatat tcatcacgct acccatgatt tttgttatgc
      901 gtgtcaatgt gccaatcgcc acgcccatcg tcagtaatgc cgcaccagtt ttcgcgatgg
      961 tttttacgac ctcaggattt tctctgacaa acgtgcgcac ttcttccaga aaaggcttca
     1021 tctcttttat gccttcattg agtgaaggca ggaaggtttc ccccagcgtg gaagaaatcg
     1081 cattgatctg gttctgcagc agcaacaact gattttctgt cgtcgcggca cgggcggcat
     1141 attctttctg cattgagccg ccatactgct gcgcatccgc cacacgatta aagttggttc
     1201 gcaacaaatc cagattcgtg agcagcggcg caatggcccc cagggattcc ttcccgaaca
     1261 gcgctttcag cacagcagcc tgtttttctt tcggcacttt tgccagagaa tccagtacgt
     1321 gcagcatggc cccgcgggcg tctttctgca tatccgccgc cagttttttc gggctaatgc
     1381 gcaacgagcg gagcactttt ttctgcgatt tggtggcaga atccctcgct gtcagcgaca
     1441 acataaaatt ctttatcccc gtcgcagcaa tttctgactc cacccccatt ccggcaatgg
     1501 ttgcccccat tgcggcaatc tctccggagg ccacacccgc aacactgcct aaagggccaa
     1561 tacgggtcac aacatcagaa atctttttcg cacttgcagg accggtatta ccaagatagt
     1621 taatcttatc cgcaagccct gccacctctc cctgtgtcag tttaaaggca gtgcgccact
     1681 gcgccatcat ctgaccggat tcttctgccg tggtgtcaaa ggccacgccc atcttcacgg
     1741 catcgtcagt aaactgcatc agctcgtcac gggcgatacc agcctgacca cccgccgcca
     1801 caatttcggc gataccttcc gccgacatgg gcagttctgt tgacaggtcg cgcacctgct
     1861 ccgtcattgc cttaaacgct tccggcgtat ccagaccatc caccactttc cggacatccg
     1921 ccatttttga ttcaagggca atggcagatt tgaccgggag cgccagcgcc cccagtactg
     1981 cggttccggc acccgctgcg cccagtaaca ggctggcaaa ctccttttta aaacctttca
     2041 gctggcgctg cattcccttc agcggtgccg ataccttatc cacggcagtg atgatcgcct
     2101 tcagctgaaa actgtcagcc atgcttcatc tcctcgttaa tgcggacggc ctcggcttcc
     2161 agttctgtaa actgcgaaac agccccccgt cgcagctcca gtggattcag tttccagaac
     2221 caggcaacat tgtagagtcg tttccggagg tgcttcccgt ctccgactgg gtaaaaaaac
     2281 gcaggatctg catgctggtc ttaaaaatat ccagttttgc catctgcgcc gccgatgaac
     2341 gcgggatccc cgccagcaac gggatatatt tcagtgccac ctggctgtcc agtttaatac
     2401 tgccttcacc ggaaataatg aaaggaaaac ccagcgcctc gatttcgtca tacgtgggct
     2461 cgcgcaactc cagcacatgc agcatttcgt tatgcgctgt caccggtttt tttaaaacta
     2521 tttctgtaac gttcattgcc attactgata ccctccctct tcgccgtgga attcaagatc
     2581 tgccgtgcct tcttcggcat tatgattagc ctctccgtgc agccatgccg ccgaaagcac
     2641 atacaccatg ccgtttgcca gctcagcggt aatcgtcatc tggtctgagg tggtaatttt
     2701 gtcgaccgga aagtttttgg gcactttaaa cgtgccctta acgtaaggtg accgccaggt
     2761 ctccttgtaa tccacatccc ctgccatgcc gacgatgtca tcccggacat tggtgttcat
     2821 cggcacttca atgccacctg tcagcgataa ctgctgacca tccactttaa aaaaacaggt
     2881 tccggcgatc tttgccatta tgctgactcc tctgaatact gaagacggaa ctggttaacc
     2941 accgcaaaga cacgtaactg attaacataa tccggcggga acagcgtatt cagtcggttc
     3001 gggttatctg catcacgctc aactatcaga tactgtttaa acagatcgta attttccaca
     3061 ataccggcac gctccatctg acgatatgtc gccagaagtt cccctttgat aacggcagga
     3121 gtgacaatcg cctgccccgg accaaaacgg gtaccatcat ttgccagctt gtgacgtccg
     3181 tacttgcttg tgatgaccga tttcagtttg cgaagaacat atgcactggt atgcagagtt
     3241 tcactgtcca gatagctgtt gtctgccacg ccatacgcat tctttttgta ggtggttacg
     3301 gaacgctgga tctgcaacgt gccgccttcc acataagccg tcgccacgcc gtgagataaa
     3361 agggtctgct gctctgtcat gatgaatcgc ttacctttcg gtgccggaag catccccacc
     3421 agctcccccg tctgtgtcgg acgggcagga tcattccgga taaataccgc ttcacgggca
     3481 aggcgactgg caaccagttc atcgacaggc gactgggttt ctttttcgta accggcaagc
     3541 gtgatatgtt gctgattatg catatctccg gcaccaacca gctctgacag cgttcccagt
     3601 tttgcggtat agacatgccc gtataactgg cgcgcataac tccagcgacc gctgctgtca
     3661 ttcatttcgg tcatcatcat attgatggag gcggcatcgt tgaacggcag accgataaag
     3721 tcgaatgcct catcgcccat agcagcaaca gcggcggtaa gatcaggcgc tccgctaccg
     3781 gcagcaccga cttccgtcac cacccgaagc cctgctggca gaatctcacc accaccagaa
     3841 ccataataat tcagacagac cggtaactca ttaccataca gccccttatg acgggcagtc
     3901 agcgtcacca cacctgcatc agatgacgcc gtaaacggca gggtgataac cccgtttacc
     3961 gcttccttaa tcgcggtggc aaccgcagtg gcatcatcgc cattcaccac aggcacctgt
     4021 acacgggagc gcccgacata cagactcagg gtgccgcttt cctctgcttc tccggcaacc
     4081 gtcaccctga ccgtcgccgc cgcccctctg gcttccggta ctgcaataac atacagttca
     4141 ccgaaaggat ctgtctgacg gtagacttcc accatacgcg ccagctggct ccccgcccca
     4201 caaatctggc gggcataatc tgccgatggc atcagcacca ggctgttaac ctcaatggct
     4261 gcatcgttgc tggcatgccc gatcagtaat gcaggcgcgc tggtcaccgc tgtatttgcc
     4321 gcagaattgt tcatctcggc gtagaacaac ggcaccagcg tattcgacgg aacagtatta
     4381 aaacttatcg tcatgatttt ttagcctta
//
LOCUS       DAZPDU010000262         4396 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZPDU010000262 DAZPDU010000000
VERSION     DAZPDU010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.266"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..590)
                     /locus_tag="ACHURS_004249"
     CDS             complement(<1..590)
                     /locus_tag="ACHURS_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFR1239832.1"
                     /translation="MADSQFYRPGLPQLISMIRSDLLTRFEQDTLLRRMDAEVYARVQ
                     AAAVHTLYGYIDYLARNLLPDVCDEDWLYRHARIKRCPRKEAVAARGFVRWDGVEGTP
                     VLPAGTQIQRDNQVTFTTTATVTAADGLLRVPVVADEPGSAGNTDDGIAMQLGTPVSG
                     LPSTGYADTITDGEDIENLEIWRARVMERYYYIPQGG"
     gene            complement(583..993)
                     /locus_tag="ACHURS_004250"
     CDS             complement(583..993)
                     /locus_tag="ACHURS_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HFR1239833.1"
                     /translation="MMINVNGRPVSTGASIDLLTRAVIISLFTWRRAGRDDAPRIFGW
                     WGDTWPAVQNDRTGSRLYLLRRSKLTNKTPQLARDYVREALAWMVEDGVASRLDINAV
                     RTGTDSLALAITIYQRDGNIHNIIFDDIWSELNG"
     gene            complement(999..1532)
                     /locus_tag="ACHURS_004251"
     CDS             complement(999..1532)
                     /locus_tag="ACHURS_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFR1239834.1"
                     /translation="MNGFSLRNLISRAVITAVDSARKCQSVGLKMIAGDQKQHVEHLE
                     PYGFTSAAQNGAEGVALFPAGDRSHGVVVVVADRRYRLKGLKRGEVALYDDLGQSVVL
                     TRSGIVVDGAGKAIIFKNAPKARFEMPVESTADITDNCDSGGLSMQQMRQAYNAHKHT
                     ENGDGGGITDTPDQPMG"
     gene            complement(1510..2586)
                     /locus_tag="ACHURS_004252"
     CDS             complement(1510..2586)
                     /locus_tag="ACHURS_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HFR1239835.1"
                     /translation="MNDTVLLRVSGREWGGWTSVRISAGINRIARDFNVAITTRWPGS
                     RDYQPRIKNGELVEVLIGDEPVLTGYVEALPLRYDASSVSMGIVGRSKTADLVDCSAL
                     PLQQSGKNLLRIVSELAAPFGITVVDAGVPQTAVIDAQPEHGETVADCLNRLLGQVQT
                     LAYDDECGRLVLGKPGTGKAATALVLGENILSCDTERSIRERFSEYQVSGQRPGNDDD
                     FGEATIAAIRQTIQDSGVTRYRPLLIQQSGTATTATCKARCEFEARQRAALTRETTYT
                     VQGWRQGSGALWRPGLSVIVFDPLNNFDNDELVIAEVTYNQDDRGTTTELRVGPADAY
                     LPEPVTARKKKMLRRISDERVFSS"
     gene            complement(2583..3974)
                     /locus_tag="ACHURS_004253"
     CDS             complement(2583..3974)
                     /locus_tag="ACHURS_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HFR1239836.1"
                     /translation="MDLPSLPSLSSLFSASSGINWRDNLYDASFRGVPFSVESDEGSF
                     GRRVQVHEYPNRDKPYTEDLGRATRRLTINAYLVGDDYAEQRDRLITAIETAGPGTLI
                     HPQFGEMQGCIDGQVTVSHSGTEGRMCRVSFQFVESGELSFPVAGAATARKLEESSVF
                     LDELIEDMFGDFDLAGIPDFIQNDVIARTTDMLGTVQTAFKMVNSAVSAGARLLQGDL
                     SVILMPPSVASDFVHTLQDTWRAGTRLVDNTQDLVQSITTMSGITLDPGLAPRAVWPT
                     DSASVIRQKQQTNLVAAVIRTTAISEATRAVTSLPQPGSLVKNQQAVVAVGGSTERQS
                     DIIHVSHPALDSVAASTGQDERAQPPTRENLTIIRESLNAAIEQELRRTADDRLFFQL
                     TSLRTELNRDIQARLVQTEETAERTPAEVLPALVLAASWYDDASRETDILDRNAISHP
                     GFVPVRALRVPVR"
     gene            complement(4021..>4396)
                     /locus_tag="ACHURS_004254"
     CDS             complement(4021..>4396)
                     /locus_tag="ACHURS_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239837.1"
                     /translation="TSADNDVDLDTDGFRQLRISCPSPSASGAIILRKASYEFGKSIG
                     VFDFANNASPATMTFLIPIYKDDDFDSDTVGFTFAFKMPRGQFFVDVKENGNIRAGVN
                     VNGESGVTYENCKLNMKDINDD"
BASE COUNT         1052 a         1281 c         1039 g         1024 t
ORIGIN      
        1 cccccctgtg gaatgtagta ataacgttcc ataacgcggg cacgccatat ttccagattt
       61 tcaatatctt caccgtctgt aatggtgtca gcgtaccctg tggacggcag accactgacg
      121 ggtgttccca gttgcatggc aataccatca tccgtattcc ccgccgatcc cggttcgtct
      181 gccacaacag gcacccggag aagaccatcg gctgcggtca ccgtcgccgt cgtggtaaag
      241 gtcacctgat tatcacgctg gatctgcgtt cccgctggca ataccggcgt cccctctacg
      301 ccatcccagc gcacaaatcc ccgggctgcc accgcttctt ttcgcgggca gcgtttgatt
      361 ctggcgtgcc ggtacagcca gtcttcatca cacacgtccg gtaacagatt tctggcaaga
      421 taatcgatat acccgtacaa cgtgtgtacg gctgcagcct gtacacgggc atacacttcc
      481 gcatccatac gacgaagcag cgtatcctgc tcaaagcggg ttaataaatc gctccggatc
      541 atagaaataa gttgcgggag gccggggcga taaaactgac tgtcagccat tcagttcact
      601 ccagatatca tcaaaaataa tgttgtgaat attgccgtca cgctggtaaa tggtaatggc
      661 aagtgccagc gagtctgtcc cggtccggac agcgttaata tcaagacggg aagcaacacc
      721 atcctccacc atccacgcca gcgcctcacg gacataatct ctggcaagct gcggggtttt
      781 atttgtcagc ttgctgcgtc gcaacagata caaacgcgac cccgtgcgat cattctgaac
      841 cgcaggccag gtatcccccc accatccaaa tatccgtggt gcatcatcac gcccggcacg
      901 ccgccatgta aaaagcgaaa taatcacagc acgcgtcaga aggtcgatcg aagccccggt
      961 cgacacgggt cgcccgttaa cattaatcat catgattttc agcccatcgg ttgatccggc
     1021 gtgtcagtga tcccgccacc atcaccattt tcggtatgtt tgtgggcatt gtaggcctgc
     1081 cgcatttgct gcatgctgag tccgccactg tcgcaattgt cagtaatatc ggcggtggat
     1141 tcgactggca tttcaaagcg cgctttaggc gcgtttttaa aaattatggc cttcccggcc
     1201 ccgtccacca caataccgga acgggtcagg acaacggact gtcccagatc gtcataaagc
     1261 gccacttccc cacgtttcag tcctttcagc cggtaacgtc tgtcagccac gaccacaacc
     1321 acaccatgag aacgatcgcc cgccggaaat aaagcaacgc cctcagcacc gttctgtgcg
     1381 gcagatgtaa aaccataagg ttcaaggtgc tcaacgtgtt gtttctgatc tccggctatc
     1441 attttcaacc ctacagactg acactttctg gcggaatcca ccgccgtgat gacagcccgg
     1501 gaaatcagat tacgaagaga aaacccgttc atcagaaatc ctcctcaaca tttttttctt
     1561 cctggcggta acaggctcgg ggagataagc atctgccggg ccaacccgta attcagtcgt
     1621 cgtgccccgg tcgtcctggt tataggtaac ttctgcgatc accagttcat cattatcaaa
     1681 attattcagc gggtcgaaaa cgatgacaga taaccccgga cgccataacg cgccactgcc
     1741 ctgccgccag ccctgaaccg tatatgttgt ctcacgggta agcgcagccc gttggcgcgc
     1801 ttcaaattca caacgggcct tacaggttgc tgtcgttgct gtgcctgact gctgaatcaa
     1861 caaagggcga taacgggtca cgccactgtc ctgaatggtc tgacgtattg cggcaatggt
     1921 ggcctcacca aaatcatcgt cgttgcccgg gcgctgccca ctgacctgat attcagaaaa
     1981 ccgctctctg atgcttcttt ccgtgtcaca ggaaagaata ttctctccca gcaccagcgc
     2041 cgtcgccgct ttacctgttc cgggttttcc cagaaccagt cgcccgcatt catcgtcata
     2101 agccagcgtc tgaacctgcc ccagtaaccg gttaagacaa tcggcaacgg tttcgccgtg
     2161 ttccggctgg gcatcaatca ccgctgtctg cggcacgcca gcatcaacaa cggtgatgcc
     2221 aaatggcgca gccagttcac tgactattct gagcaggttt tttccgctct gctggagtgg
     2281 caaagcagag cagtcaacca gatcggctgt tttgcttcgc ccgacaattc ccatgctgac
     2341 gctgctggcg tcataacgaa gcggtagtgc ctccacatat ccggtgagca caggctcatc
     2401 cccgataagc acttcaacca gctcaccatt ttttatccgg ggttgataat cccggcttcc
     2461 gggccagcgg gtggtaatgg caacattaaa atcccgggca atacggttaa tgcccgcact
     2521 gatacggacg gatgtccagc cgccccattc gcgaccggaa acccgaagta aaacggtatc
     2581 attcatctga cgggtaccct taatgccctg accggaacaa agcccggatg agagatggca
     2641 tttcgatcca ggatatcagt ttcacgggac gcatcgtcgt accatgacgc agccagaaca
     2701 agcgcaggca gaacttccgc tggcgttcgc tctgcggttt cctccgtctg aaccagacgc
     2761 gcctgaatat cccggttcag ttctgtacgt aatgacgtca gctgaaaaaa cagcctgtca
     2821 tccgccgtac gcctgagctc ctgttcaatc gccgcgttca gcgattcgcg tatgatggtg
     2881 agattttccc gcgtgggtgg ttgcgctctc tcatcctgtc ctgtgctggc tgccacgctg
     2941 tcaagtgccg gatgagaaac gtgaataata tcggactgac gttcagtgga accaccaaca
     3001 gccacaaccg cctgctgatt tttcaccagg cttccgggtt gtggcagtga agtgaccgcc
     3061 cttgtggctt cgctgattgc cgtcgtccgg atgacagcag ccacaaggtt tgtctgctgt
     3121 ttttgcctga taaccgatgc ggaatctgtg ggccacaccg cacgcggtgc cagtcctggg
     3181 tccagcgtaa tgccggacat tgtcgttatg gactgcacca gatcctgtgt gttatccacc
     3241 agccgggttc cggcccgcca ggtatcctgc aacgtatgca caaaatcact ggcaaccgac
     3301 ggcggcatca gaatgacgga taaatcgccc tgcagtagtc tcgctccggc agaaacagcc
     3361 gaattaacca ttttgaaagc tgtctgaacg gttcccagca tatcggtcgt ccgggcaata
     3421 acatcgttct gaataaagtc cggtattccc gcgagatcaa aatcgccgaa catgtcttca
     3481 atcaactcat ccaggaatac ggacgactcc tccagttttc tggcggttgc agctccggcg
     3541 accggaaatg acagttcccc gctctcaaca aactgaaatg aaacccggca catacggcct
     3601 tcagtgccgg agtgagaaac ggtcacctgc ccgtcaatac agccctgcat ttcaccgaac
     3661 tgcggatgga tcagcgtccc cggcccggcg gtttcaatcg ctgtaatcag cctgtcgcgc
     3721 tgctctgcgt aatcatcacc aacgagatac gcattaatcg tcagccgtcg cgtggcacgc
     3781 ccgagatctt ccgtgtacgg tttgtcacgg tttgggtatt catggacctg aacgcggcgt
     3841 ccgaacgaac cctcgtcgct ctccaccgaa aacggaacgc cacgaaatga agcgtcgtaa
     3901 agattatcgc gccaattgat acctgaagat gcagaaaaga gtgaagaaag agaaggtaaa
     3961 gaaggtaagt ccatactgtc tccactataa aaaaaccgcc aatttctggc ggcttatata
     4021 ttaatcatca ttaatatctt tcatattcaa tttgcaattt tcgtaggtca ctcccgactc
     4081 gccattaaca ttaactccag ctctgatatt cccgttttct tttacatcta caaaaaactg
     4141 gcctcttggc atcttgaacg caaatgtaaa accaacagta tctgaatcaa aatcatcatc
     4201 tttatatatt gggatcaaaa atgtcatcgt cgctggtgat gcattgtttg caaaatcaaa
     4261 tacacctata cttttgccga attcataaga agcttttctc aatataatcg caccgctggc
     4321 agaaggggat gggcacgata tcctcagttg tctgaatcca tctgtatcca agtcaacgtc
     4381 attatctgca gatgtt
//
LOCUS       DAZPDU010000263         4364 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZPDU010000263 DAZPDU010000000
VERSION     DAZPDU010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.267"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..215)
                     /locus_tag="ACHURS_004255"
     CDS             complement(<1..215)
                     /locus_tag="ACHURS_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR1239838.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDT"
     gene            complement(330..405)
                     /locus_tag="ACHURS_004256"
     tRNA            complement(330..405)
                     /locus_tag="ACHURS_004256"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(370..372),aa:Thr,seq:ggt)
     gene            complement(412..486)
                     /locus_tag="ACHURS_004257"
     tRNA            complement(412..486)
                     /locus_tag="ACHURS_004257"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(451..453),aa:Gly,seq:tcc)
     gene            complement(603..687)
                     /locus_tag="ACHURS_004258"
     tRNA            complement(603..687)
                     /locus_tag="ACHURS_004258"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(651..653),aa:Tyr,seq:gta)
     gene            complement(696..771)
                     /locus_tag="ACHURS_004259"
     tRNA            complement(696..771)
                     /locus_tag="ACHURS_004259"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(736..738),aa:Thr,seq:tgt)
     gene            1133..2083
                     /gene="coaA"
                     /locus_tag="ACHURS_004260"
     CDS             1133..2083
                     /gene="coaA"
                     /locus_tag="ACHURS_004260"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFR1239839.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2112..3077)
                     /gene="birA"
                     /locus_tag="ACHURS_004261"
     CDS             complement(2112..3077)
                     /gene="birA"
                     /locus_tag="ACHURS_004261"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFR1239840.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3074..4102)
                     /gene="murB"
                     /locus_tag="ACHURS_004262"
     CDS             complement(3074..4102)
                     /gene="murB"
                     /locus_tag="ACHURS_004262"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFR1239841.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
     gene            complement(4237..4352)
                     /gene="rrf"
                     /locus_tag="ACHURS_004263"
     rRNA            complement(4237..4352)
                     /gene="rrf"
                     /locus_tag="ACHURS_004263"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1112 a         1095 c          983 g         1174 t
ORIGIN      
        1 gtgtcgtatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
       61 ttatcgatct ggtcgaatgc gcgagcagca ccgccgtagg ttttagccag tacggtagtg
      121 attgcagcgg tcagcgttgt tttaccatgg tcaacgtggc cgatagtacc gacgttaacg
      181 tgcggttttg tacgttcaaa cttttcttta gacatcgatt gtccctctaa gacacggata
      241 aatcggtgat atcaccacat caaccaggca acatgcccga cttgttgaat gcaataaaca
      301 gaaggaaaaa acagggagga gaaaagaagt ggtgctgata ggcagattcg aactgccgac
      361 ctcaccctta ccaagggtgc gctctaccaa ctgagctata tcagcacatc ttggagcggg
      421 cagcgggaat cgaacccgca tcatcagctt ggaaggctga ggtaatagcc attatacgat
      481 gcccgcatcc tggaactcgg ctacctgatt ttcattctgc actgaatatc gagagaagct
      541 ctctttattc gagccggtaa gcgaacttat cgtctcgggc tacgccatcg cgtggccgaa
      601 attggtggtg ggggaaggat tcgaaccttc gaagtctgtg acggcagatt tacagtctgc
      661 tccctttggc cgctcgggaa ccccaccgga cttgatggtg ccgactaccg gaatcgaact
      721 ggtgacctac tgattacaag tcagttgctc tacctactga gctaagtcgg catcaagtag
      781 cgcgcattct atggagacat gcgagttcat gcaactaaaa aattgcataa tttgttttat
      841 tggtcacatt ttatgcgaca cgatgaagaa acagccgaac ggagggcaga ttagcacact
      901 ttttcaacat cattgtgctc aacaatgcgc tcctgctaaa ccataattct ttttatcaga
      961 tggaatatct gtcacattgc ttttcaacga tagcttcctg gcagagattt tttcttatta
     1021 ttcctcccca tctggtgtta ccctcctgcc cattaaccca ttcaacagaa ctgtgacgcg
     1081 ccatggcaaa tatcgctttg ccgatagagc tatgaccgcc agaaacatgc ttatgagtat
     1141 aaaagagcaa acgttaatga cgccttacct acagtttgac cgcaaccagt gggcagctct
     1201 gcgtgattcc gtacctatga cgttatcgga agatgagatc gcccgtctca aaggtattaa
     1261 tgaagatctc tcgttagaag aagttgccga gatctattta cctttgtcac gtttgctgaa
     1321 cttctatata agctcgaatc tgcgccgtca ggcagttctg gaacagtttc ttggtaccaa
     1381 cgggcaacgc attccttaca ttatcagtat tgctggcagt gtcgcggtgg ggaaaagtac
     1441 aaccgcccgt gtattgcagg cgctattaag ccgttggccg gaacatcgtc gtgttgaact
     1501 gatcactaca gatggcttcc ttcaccctaa tcaggttctg aaagaacgtg gtctgatgaa
     1561 gaagaaaggc ttcccggaat cgtatgatat gcatcgcctg gtgaagtttg tttccgatct
     1621 caagtccggc gtgccaaacg ttacagcccc tgtttactcg catcttattt atgatgtgat
     1681 cccggatgga gataaaactg ttgttcagcc tgatatttta attcttgaag ggttaaatgt
     1741 cttacagagc gggatggatt atccacacga tccacatcat gtatttgttt ctgattttgt
     1801 cgatttttcg atatatgttg atgcaccgga agacttactt cagacgtggt atatcaaccg
     1861 ttttctgaaa ttccgcgaag gggcttttac cgacccggat tcctattttc ataactacgc
     1921 gaaattaact aaagaagaag cgattaagac tgccatgaca ttgtggaaag agatcaactg
     1981 gctgaactta aagcaaaata ttctacctac tcgtgagcgc gccagtttaa tcctgacgaa
     2041 aagtgctaat catgcggtag aagaggtcag actacgcaaa taatttgcag gggagcgaat
     2101 actccccttt cttatttttc tgcactacgc agggatattt caccgcccat ccagggtttt
     2161 attattccat cctgctcaag taataaagcc ccctgtttgt ctattccgcg tgaaatgcca
     2221 aatatttctt tatcaccaat gataagtttc actgggcgat taataaaatt atccagcttt
     2281 tcccagcgcg acagataagg tgccaatcct tcttgttcga agagttccaa cgcagcacgt
     2341 aattcacgta ttagcatggc cgccaacgta ttacgatcga gattgatccc cgcttcctgc
     2401 agcgtgatcc acccctgatt aacgacactc tcttcaacac ggcgcattgc catgttgatc
     2461 ccggctccaa tgactatttg cgccgcatcg ccagttttgc cagtcagctc cacaagaatg
     2521 cctgccagct tgcgatcctg cagatagagg tcattaggcc atttgacacg aactttatct
     2581 gctcccagct tgcgtaatac ttccgccatc acgataccga taaccagact taaaccaatc
     2641 gccgccgccg ggccttgttc cagacgccag aacatcgaca aatataagtt tgcgccaaaa
     2701 ggcgaaaacc atttccgccc ccggcgacca cggccagcct gctggtattc tgcaacacag
     2761 gcatcgcccg atttaagctc tccgatacga tcaagaaggt actgattcgt ggagtcaata
     2821 actggcagca cggctacact accgccatcc agctgaccca atatctgttc agcattaagt
     2881 aactggatgg gctcaggcag gctgtatcct ttacccggaa cggtaaagac atcaacgccc
     2941 cagtcacgca gtgtctgaat gtgtttatta atagccgccc ggctcattcc cagcgtttca
     3001 cccaactgct cgccagagtg aaattcaccg ttcgctaaca gggcaatcaa tttcagtggc
     3061 acggtgttat ccttcatgaa attgtctcca ctgcgctcac ttcacctgat gcaccaataa
     3121 agcggacttc aggctcaagc cagacattaa atttttcacc cactttttgt cttacatggt
     3181 gtgccagttg caccacatct tcgctttttg cattgtcttc attaatgaga actaacgcct
     3241 gttgacggtg caccgcagcc ccacccattt gcatcccttt tagctggcac tgatcaataa
     3301 gccaacctgc tgccagtttt actgaaccac ccgcctgggg gtaatttggt gctgttggaa
     3361 attgcgccag taatgcttta gccgtttcgg cagatacaac agggtttttg aagaaactac
     3421 cggcattgcc attcactttt ggatcaggga gtttggtggt gcgcatatga cacaccgcat
     3481 taaatacttg ctgtggcgtt actgttgtag gatccagacg agttaagtca ccatacgtta
     3541 gtacaggttg ccactctttt ggcagacgca gacctacggc tacaatggcg aagcgatcct
     3601 ggtattcatg tttaaaaata ctgtcgcgat agccaaaacg gcactctttg gcagttaagc
     3661 gcacttgctt gcctgtcgcc agttcaacac aatcaacata agcgcaaact cgctgtaatt
     3721 ctacgccata agcaccaata ttctggatag gtgatgagcc gacacaacca ggaattaatg
     3781 ccagattttc cagaccaggc ataccttcct gcaaagtgta ttttaccagg cgatgccagt
     3841 tttctccggc tcctacatgt aaataccacg catcaggttc atcatgaatt tcgatacctt
     3901 tgatccggtt gatgatcacc gtgccacgat agtcttccag aaaaagtaca ttacttcctt
     3961 cacccagaat aagaacgggt tgtccttctg cggttgcatg ctgccaggca ttgagtaatt
     4021 gttgttcgtc ttcggcacat acaatgtgct gagcattatg atcaatgcca aatgtgttcc
     4081 agggttttaa ggagtggttc atagctgctt tcctgatgca aaaacaaggc tagtttaccg
     4141 tatctgtggg gggatggctt gtagatatga cgacaggaag agtttgtaga aacgcaaaaa
     4201 ggccatccgt caggatggcc ttctgcttaa tttgatgcct ggcagttccc tactctcgca
     4261 tggggagacc ccacactacc atcggcgcta cggcgtttca cttctgagtt cggcatgggg
     4321 tcaggtggga ccaccgcgct acggccgcca ggcaaattct gttt
//
LOCUS       DAZPDU010000264         4339 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZPDU010000264 DAZPDU010000000
VERSION     DAZPDU010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4339
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.268"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            213..365
                     /gene="hokA"
                     /locus_tag="ACHURS_004264"
     CDS             213..365
                     /gene="hokA"
                     /locus_tag="ACHURS_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFR1239842.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     ACNLKE"
     gene            complement(342..413)
                     /locus_tag="ACHURS_004265"
     CDS             complement(342..413)
                     /locus_tag="ACHURS_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239843.1"
                     /translation="MRNAVRKAGITSRQRLLLFQIAG"
     gene            complement(553..765)
                     /gene="cspA"
                     /locus_tag="ACHURS_004266"
     CDS             complement(553..765)
                     /gene="cspA"
                     /locus_tag="ACHURS_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFR1239844.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(1046..1336)
                     /locus_tag="ACHURS_004267"
     CDS             complement(1046..1336)
                     /locus_tag="ACHURS_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFR1239845.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1770..2480
                     /locus_tag="ACHURS_004268"
     CDS             1770..2480
                     /locus_tag="ACHURS_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFR1239846.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2530..3504)
                     /gene="ghrB"
                     /locus_tag="ACHURS_004269"
     CDS             complement(2530..3504)
                     /gene="ghrB"
                     /locus_tag="ACHURS_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFR1239847.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLHATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3608..4267)
                     /locus_tag="ACHURS_004270"
     CDS             complement(3608..4267)
                     /locus_tag="ACHURS_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFR1239848.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLTEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
BASE COUNT         1081 a         1037 c         1058 g         1163 t
ORIGIN      
        1 tatgaggttt ttttaggatt atatcaagga gaagaaacaa acttattaag ctagaatagc
       61 cacgggtgct tgagactgtt tgtctcaggt attcaccgaa aggcagacag agaaaagccc
      121 cacctgacta taaatcaaag tgaggctacc ctatgcctga acaccataag gttagcctct
      181 tactcgttgg aaatcaacac aggaggctgg gaatgccgca gaaatataga ttactttctt
      241 taatagtgat ttgtttcacg cttttatttt tcacctggat gataagagat tcactgtgtg
      301 aattgcatat taaacaggag agttatgagc tggcggcatt tttagcctgc aatttgaaag
      361 agtaagagtc tttggcggga agttattccc gcctttctta cggcgttgcg cattctcatt
      421 gcacccaaat ttattcttca caaaaataat actaaatttt attacgcgat cgattattta
      481 tttcctgaaa aacaaataaa aaaatccccg ccaaatggca gggatcttag attctgtgct
      541 tttaagcaga gattacaggc tggttacgtt accagctgcc gggcctttag cgccgctttc
      601 gatggtgaag gacactttct gaccttcgtc cagagatttg taaccatcgt tctggatagc
      661 agagaagtgt acgaacacat ctttagagcc atcgtcagga gtgatgaagc cgaagccttt
      721 gtcagcgttg aaccatttta cgataccagt cattttaccg gacatagtgt attaccttta
      781 ataattaagt gtgcctttcg gcgatatggc gtgctttaca gattttgaag cgttaaagga
      841 atgtgcacta cgaggggtat caacgataac tcttgaaggg acttgcctta ctacactgct
      901 ttaatggtct gtacgtcaaa ccgttgatgt gcattaagcc acgcattggc gggtgatgca
      961 acaattattt ttcatattta tgattaatcg gttggaaaac ggtcctgtca tcaggaccgt
     1021 aaacagcaat aaagtggata aaagtctatt ccatcaactg cttacttaat gccgggttgg
     1081 cttgaatcaa acgcatcaat tttagttcgg cacttgaagg cttcacgcgt ctggattccc
     1141 attcctttac catggcgact gatacgccca aaacccgggc gaaatcatcg atttttaacc
     1201 ctgtcccttt tcgtaactgc tcaatttcgg tacaggacgt tgttctgtgc gtcagggtaa
     1261 ttttctgcgt ttcatcttta aaaacaatct gttccaggct gctcaacagc tcatgcattg
     1321 gatctttata ttccattgaa aactcctcaa atcacactgc gggatcgtga attgcatcga
     1381 agctcattaa gaatagtcgg gaaaagcaga cacagcgggg tgaagtcagg gcatgctctg
     1441 gctatgattc gattcagcct gtcatttagc gtgacttata gccacctaac gatctgataa
     1501 atgccgatat atctcttacc actcctgttt tgtaaagatt tcgaaaatct aaccttctgc
     1561 gaaggcgtaa atctctggct acaaaaaagg caacggcact atcatttttt aacaatgaaa
     1621 caacaaatta accttgtttc attttctcca tgattttacc aggaagatta cgtagaaagg
     1681 gtatcctgca agcctgtcct ggactatcct tgtcacgtca gacacgcgtg tgtcgttgtg
     1741 cgcttttttt gggtgaaagg agtaagaaaa tggcgacagg aaagtcctgc tctcgctggt
     1801 ttgcgcctct tgcggcgtta ttaatggtag ttagcctgag tgggtgtttt gataaagaag
     1861 gcgatcagcg taaagcgttt atcgacttcc tgcagaatac agtgatgcgt agcggtgaac
     1921 gtctaccaac cctgactgcc gatcagaaaa aacagtttgg tccttttgtc tctgattacg
     1981 cgattctgta tggttattct cagcaggtga atcaggcgat ggattctggt ctgcgcccgg
     2041 tcgtagacag cgttaacgca attcgcgtac cgcaggatta cgttacgcaa agcggtccgc
     2101 tgcgtgaaat gaatggttcg ctgggtgtgc tggcgcaaca actgcaaaat gcgaagttgc
     2161 aggccgatgc tgcgcactct gcgctaaaac agagcgatga cctgaaaccg gtatttgatc
     2221 aggcattcac caaagtggta acgacgccag ctgatgcatt gcaaccgttg attccggcgg
     2281 cacaaacttt cacgcaacaa ctggtgatgg ttggagacta cattgctcag caaggtactc
     2341 aggtgagctt tgtcgcaaat ggcatccagt tcccgacttc acagcaggca agtgaatata
     2401 acaaactgat tgcgccatta ccggcacaac atcaggcgtt taatcaggcc tggactacag
     2461 cagttactgc aacccaataa agagtaaaag cccgagcgat tatcgggctt tacttcacgc
     2521 agtcgcggct tagtccgcga cgtgaggatt cacacagttc ttctcaacct ttccttgtaa
     2581 cgcatcaatt aaattatcca cggcacaggc ggccatgcca taacgcgtct catgggtggc
     2641 agatccaata tgcggtactg cgacgacgtt ggccattgag agcaacggcg aatctacgga
     2701 cagtggctct tgttcgaaga catcaagccc ggcggcgtga atttcccctt tctgtaatgc
     2761 tgcgatcagt gcattttcgt caaccaccgg gccacgtccg gcattaatga aaatggcgga
     2821 ggatttcatt ttggcgaatt gttctgcgcc aaacagatga tgcgtctcat cagttaacgg
     2881 caggatcagg caaacgaaat ctgactcttg taacagtgta tccaaatcgc agtagcgggc
     2941 gttgaagcgt tcttctgctt ctttatggtg gcggcgcgcg ttatagagga tgggcatgtt
     3001 gaagccaaag tgcgcacgtt gtgccagcgc cataccgatc cgtcccatcc cgacaatgcc
     3061 cagtgtttta tggtgaacgt cagtgccgta ccagtccggg cctatgctcg cggtccattc
     3121 gcctgctttt acccgttctg ctacctccac aacccgacga gcggtagaca acaccagcgc
     3181 catcagcgta tcggcgacgg tttctgttaa tacggttggc gtgtgcatca gcagaatttt
     3241 tcgggcggta agcgcatcga catcaaaatt gtcatagccg acggagatcg ttgatgtggc
     3301 atgcagtttc ggcatttttt ccagcaatgc ggcatcaaca ttctcgtttg aacccagtaa
     3361 accttcagct tcggcaaaaa ttgctgcatt ttgttcgacg gtttgtgggc tgaggtttgc
     3421 cacctggtga acggtgaaat gctcttgcag gcgttgcagt aaatcatcag gtaaggcttt
     3481 gtagaggata acggacggct tcatgcttct ctccattcac tgataaggcg atcaccttag
     3541 cctgaaatat gatgaaggaa aactgaagaa tgccaggtga tttctgcatc acctggcatg
     3601 aaagggatta cagcgggctt aaggtaattt ctacacggcg gttttgcgcc ttaccttctg
     3661 cggtgctgtt gctggcgatt gggttagccg ggccaaggcc ctgagtacgg atgcggctgg
     3721 cgtccacgcc ctgggtgatc aacgcgctgg caacggaatc cgcacgctgc tgggagagac
     3781 gcatgttcag gtcgtgaccc cccgtgctgt cggtataacc aatcacgtta accgccgttt
     3841 tcggatactc tgtcagcacc attgccacgc cggtcagggt gttagcgcct gccggtttca
     3901 gggtcgcgct gctgctgtcg aaggtcacat tgttcggcat attgaggata atgttatccc
     3961 cgctgcgggt tacgctaaca ccagtgccgc gcattttgtc acgcagcttc gcttcctgca
     4021 catccatgta ataacccacg ccgccgccca gagctgcgcc tgctgctgcg ccaatcagcg
     4081 cgcctttacc gcgatctttc ttcgaagaag agagcgcacc aatacccgcg cccacgagag
     4141 agcccagacc tgcgccgata gcagatttgc ctgcttcgcg ttcgccggtg taagggttag
     4201 ttgtgcagcc agataccgcc agagcgccac tcactacggc ggcaataaga taaacacgtt
     4261 tcttcattgt taatccttaa taaccttttt attctttgcc acgggttccg tggcgggaga
     4321 ttatgccgcg tgaacatga
//
LOCUS       DAZPDU010000265         4330 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZPDU010000265 DAZPDU010000000
VERSION     DAZPDU010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4330
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.269"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..583)
                     /locus_tag="ACHURS_004271"
     CDS             complement(<1..583)
                     /locus_tag="ACHURS_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR1239849.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIDLGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADAITVDAMIPNF"
     gene            complement(646..1095)
                     /locus_tag="ACHURS_004272"
     CDS             complement(646..1095)
                     /locus_tag="ACHURS_004272"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFR1239850.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(1092..4184)
                     /gene="ebgA"
                     /locus_tag="ACHURS_004273"
                     /pseudo
     CDS             complement(1092..4184)
                     /gene="ebgA"
                     /locus_tag="ACHURS_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026199.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
BASE COUNT         1028 a         1090 c         1168 g         1044 t
ORIGIN      
        1 tgaagttcgg gatcatcgcg tcaacggtga tggcgtcagc aggctgtacg ccgccaacca
       61 gcgccgtacc tgccagtaaa atgtaggaga gcgttaacag cagcatcagc gttgaagtga
      121 cggaggtaat tggccccaac attttcgccc cgttggtgga aacccaggtg gagaaggcga
      181 acagcaccat actgataatg gtggtggcaa ccggcgtcat aatatattcg tatccgagga
      241 aggcatacga agcataagca ataacgcgcg gcaacagtga ggtgaaaaag aacaggttta
      301 cgaaccagta ggtataggca gtaataaatg cccaacgtcc gcccagcgaa ctttttaccc
      361 atgcgtagac accggcttct gagtttttat ttaacgaaac aaattctgcg atgatcagac
      421 agaagggaat aaaataaaaa atcgtcgcga ggaaaaacat cggtgccgag gccagtccaa
      481 gatcaatatt attattgata acgttgttaa agctgtaaac ggcggcaaaa gtcagcgaga
      541 gcaagccgaa tttgccgatt gtattacgtt tggtatcaga cataacacat ctccgaaaat
      601 gagtagggta gcggtggcgg caaactcctt ttgccgccgt agttgttatt tgttatggaa
      661 ataaccatct tcgatggtga ctttgagaac cacttttttg accgcgttat tgcagataaa
      721 ccgatacgct tcatggatat cgcaaataac gatttgccct tcgtggacct caacggtttc
      781 tccgcagcct tttaaatatt cacggtcagt ttcatcacga taatattcca ctacctgtaa
      841 tgtctctttc ggcgcatatt caattttttg ctgcccttgc aggtagtaat gcacttcaaa
      901 atagcgtcga tgcccggtaa atagcgcatc ggtcgcggag tctgtctcca cgcggtaagt
      961 caatgagtca ccgatggagt gggcgacgcc aggctgaatg ttgtcgatat tttcaatcgc
     1021 ttcaacgcag cgttgccact tcttgccaga ggcgtaaatc tggcggaact gttctaagtt
     1081 atcgatgatc ctcattgctt attctcgctg tgcaaattcg tggaaaagaa ccctgcgccg
     1141 aactcatacg acgccaggct ttgcgcggta gcttctccgc cagaaaccgg cagcaacgta
     1201 aagccgtagc tgaagtcacg gaaccagacg cgccaggagt ccagcacctc gctgccccag
     1261 gagttggagc cgaggccaag cagctggtga tcgaggttca gggtgatgtc atcactgcgc
     1321 tgcagctcgt tacagtgctg ggcagcgtgg atgttttcct aggtatagtg ccaggcgctg
     1381 aagttaattg ggcgctgcgg aaccaccagc agaccgttac cgtggcggtt agtcagtgcc
     1441 gtccagcgga catgctgacg gttaccgttg ttctgcggga agggatagtt ctcgaacatg
     1501 gcatcgacgg tgctgcgcca gatatcgatg atgttagcct gctggctgtc ggcgtagttt
     1561 tcgcccggtc cacgaccgta atacgccacc tgatcgtatt cgccgttaat tcccatggtg
     1621 aaaccgatgc acggaatgat gtgcggatag tcgccgtaac gctcgccgga aagcgccacg
     1681 ttaacctggc catcggcagc gatgcgccag atgtaggtgc agcgcattcc gaaatcaaac
     1741 accggcgggg caataaccgt gcggctgatg atcaacactt caccctcgct ctgttctacg
     1801 gcaaagtcgc gcagatgttc ctgcatgatc tgcaaatgat tcggctgcca caacccttcg
     1861 tactcctgct tgtggttgtc gatcatcggc ttgaagaagt tgatctttgg ctcgcgggtc
     1921 agcagcgact cgccatttac ctgccaggat gtcggtttgc cactcatttt tgagaaggtg
     1981 atcgcgaagt tgtagccgcg aacggtgcag ctcaaacgat cgtcttccag cgtcagcgga
     2041 cgcgcattat ttggtgcgaa aggcactggc tgcgcggtgt tttccttcag cgggaactga
     2101 taagtggcga tagaatgccc ggcttcgctg tagcgggtgc gggaatcttt ggtcaccgta
     2161 atgttgagga acgcttcgcg ggcgtccagc tgcggcagcg tgatctgcaa gggggcttcg
     2221 ctgttcggcg caacgtcgcg cagtttaatc tgctgcgtcg cgagcgtttc accttcggcg
     2281 cgcacctctg cgtgcagggt gtagtcatca agcgtggtaa accacagttt attttcgact
     2341 ttcaactcgc cgcgagtcag atcccgcgcg tggattttta ccggcgcgat aacctgtttg
     2401 tactctttca ggcccggtcc cggcgtctga tcggaataga tcaaaccatc aagacagaag
     2461 ttatagttgt tgggatagtc gccgtagtcg ccgccgaatt tataccagac attgccgtgg
     2521 tcgtcctgtg cctggatccc gtggtcgcac cactcccaga cataatgacc ctgaatgcaa
     2581 tcgtgcttat agaagacgtt ctggtactcc gtcagcccgc ccggtccgtt ccccatcgca
     2641 tgagcatatt cacagatgat gcgcggcttc ggatgcgggt attcaccaaa ctcattcatc
     2701 agcggcacgc gggtgtacat ggtggaaata atatcgacca cttcagcatc gcgatcttct
     2761 tcgtaatgca ccagtcgcgt gtcatccagc gccttcgctg cgtggtacat cgcgcggatg
     2821 ttacagccat agccggattc attgcccagc gaccagatga tgatcgacgg atggtttttc
     2881 tgcgcgtgaa tatggcgaac aatgcgctcg acgtagactt tttcccactg cggatcgtcg
     2941 gtaatacggc tgatatcgcc gacattagca aagccgtgcg attcgacgtc ggtttccgcc
     3001 atcacaaaca ggccgtagat atcacacagt tcgtaaaaac gcggatcgtt cgggtagtga
     3061 gcggtacgca cggagttgat gttgtgctgc ttcatcaact ggagatcttt ctcgacgcga
     3121 tccattccaa cggcgcggcc tttgcgatga tcgttgtcgt gacggttgac gccgtgcagc
     3181 atcacataac ggttattgat ccagaacaga ccgtcgcgca ctttgatatc acggaagcca
     3241 acgcgttgtg gtaccacttc cagaacgttg ccgttggcgt ctttcagcgt catgaccaga
     3301 tggtaaagat aaggggattc tgctgaccat tgctgcggct gttcgacagt aaaagcaaag
     3361 ctggcgctgg tcagtttttc aattgccaaa tgatcaatgg cgctgctgtg caccacgcgt
     3421 tcgccatcaa acagggtata ttccagcgtc gtgacgacag gggaggcggc gagattttcc
     3481 agcaccactt cgcaggaaag cgtggcatcg caataggctt cgtcaaagtc ggtacgcacg
     3541 gtgaaatcgt taatatgtgt tagttgtttt ccgatcagat aaacatcgcg gaagatcccc
     3601 gctgaccacc acatatcctg gtcttccacg taggtagagt ccgcccattg catcacgcgc
     3661 acacacaaca ggttgtcgcc ggttttaacc atcgcgctga tgtcaaactc tgcggtcagg
     3721 cgactgccct tgctgaaacc cacatactga ccgttaacgt agacttcaaa ataggtttcg
     3781 acgccgtcaa atttaatcag cgtctgttta ccctgccagc cgtcgctgag ggtgaaaata
     3841 cgttgatagg caccggttgg gttatcgctg ggaacaaacg gcacatcgat ggggaacgga
     3901 aaaccttcgt cggtatattg cagtttgccg tgaccttcca tttgccacat ggcggggacg
     3961 gtaatatgcc cccagtcagc cattaactca gaggtgaagg cttcaggcac ttgcagcggg
     4021 tggtcaaaaa agtggaaatt ccactgaccg cttaagggca gaaacaggct gctggtttcg
     4081 cgggcaaagg tacgcgcttg cgcaacagaa tcatatgaaa aaaagtacgc acgtggcgca
     4141 agtcggtttt cgtgggtgag ctgaatgttt tcccagcgat tcatagggcc tcccggtaga
     4201 gagaaatcgg aaccattcat ggtgatggta gtttgtgtaa ttgctgggga gtttagtaaa
     4261 atttttacta aaaagtggca ggtaaatgca tttactttaa ctggatcacg aaaataaacg
     4321 gcagcactgt
//
LOCUS       DAZPDU010000266         4309 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZPDU010000266 DAZPDU010000000
VERSION     DAZPDU010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.270"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..1870)
                     /gene="speC"
                     /locus_tag="ACHURS_004274"
     CDS             complement(<1..1870)
                     /gene="speC"
                     /locus_tag="ACHURS_004274"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFR1239851.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATSVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDA"
     gene            complement(2033..2116)
                     /gene="yqgH"
                     /locus_tag="ACHURS_004275"
     CDS             complement(2033..2116)
                     /gene="yqgH"
                     /locus_tag="ACHURS_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HFR1239852.1"
                     /translation="MHAISLHSLAYRRFARNVSLSMLLLMR"
     gene            2269..2976
                     /locus_tag="ACHURS_004276"
     CDS             2269..2976
                     /locus_tag="ACHURS_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFR1239853.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            3082..3157
                     /locus_tag="ACHURS_004277"
     tRNA            3082..3157
                     /locus_tag="ACHURS_004277"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3115..3117,aa:Phe,seq:gaa)
     gene            3355..>4309
                     /locus_tag="ACHURS_004278"
     CDS             3355..>4309
                     /locus_tag="ACHURS_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1239854.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSNKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGS
                     YKGQPVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPI"
BASE COUNT         1114 a         1141 c         1037 g         1017 t
ORIGIN      
        1 gcgcatcctg ggggttcatc accactgacg ggaaactctg ctggcggaac atcgcttttt
       61 gtaggtcttt gacgtcgaaa ctgacataca gatcgtgcat ctcctgacac aactggcgca
      121 gggtgtagtc gcgatagcgc accggatatt tgttataaac gctcggcaac acctcagcca
      181 gcggcgagtc atcctcaata tgctgttcaa attgcgccag catcgccacc agttgtgcca
      241 gcttctcgtg gctttccgcc ggagttaata agaacagaat ggagttgaga tcgcacttct
      301 ccggcacaat gccgttctca cgcagatagt gcgccagaat cgtcgccgga acgccaaagt
      361 cgctatattc gccggtttcg gcattgatac ctggtgtagt gagtaacagc ttgcacggat
      421 caacaaaata ctgatccgcg gcatatcctt caaagccgtg ccacttcgcc cccggctcaa
      481 aactgaaaaa acggcggtcg ctggctaaca cagatgtcgg ataatcctgc cacaatttgc
      541 catcaacaac gggcgggata aacgggcgga acagcttaca gcgcgcaaga atagccttgc
      601 gcgactcaat acccaactca acgcactcag cccacagccg acgcccactc tccccttcat
      661 gaattttggc gttaacatcc agtgcagcaa acagcggata gaaagggctg gtagaagcat
      721 ggagcataaa ggcgttattc aaccgcttat gcgggcaaaa acgcgcctgt ccgcggatat
      781 ggttatcttt tttatggatc tgcgacgtct gtgagaatcc cgcctgctgt ttgtgcaccg
      841 actgagtcac aaagatcccc ggatcgtttt cgttaagttc taatagcagc ggcgagctat
      901 ccgccatcat cgggataaac tgttcgtaac cgacccacgc ggaatcaaac agaatgtaat
      961 cacacagatg cccaacggta tcgatcacct gacgggcgtt atagacagtt ccgtcatagg
     1021 ttcccagctg aataatcgcc aggcgaaacg ggcgcggcag ctcggctttt tctggcgcaa
     1081 cgtcgcgaat ttgctggcgc agatactctt cattaaaaca gtgcgcatca ataccgccaa
     1141 tgaaaccaaa cgggttgcgt gaagcttcca gatagaccga cgtcgccccc gcctgaatca
     1201 gcgcgccgtg atgattcgac ttatggttgt tacggtcgaa gagcaccaga tcgccacgcg
     1261 ttaacagcgc attcgtcacc actttattcg ctgccgatgt gccgttcagc acaaaatagg
     1321 ttttatcggc atgaaagact ttggctgcga atttctgcgc atccttcgcc gatccttcat
     1381 gaataagcag atcgcccaat tttacgtcag cgttacacat atcggcgcga aagacgttct
     1441 caccaaagaa atcgtaaaaa tggcgtccgg caggatgctt tttaaaaaac gcaccatgtt
     1501 gatgtccagg gcaagcaaag gtgctgttgc ccatctcaac gtactgcgtc agcgtgtcat
     1561 aaaacggtgg cagcaaattc tcttcatact gacaggctgc ggattccagc tccagccact
     1621 gctgctcgtt gccgttgatt accgccgtaa cgcccgcagg taattcaaca gcatgttcgg
     1681 aatacaaaaa caccggtaga tgaaaaccgg tgcgcttaag caacgcaaga atgccactgc
     1741 gactatccgc agcggtaatg acgactgccg cgacatccgt aaaatcagta tctcccaacg
     1801 ccaccacgcg gcgatgagta gaaagtcggg ataccagttc actactggcg gcaatattca
     1861 ttgatttcat aagcgcaaaa cccgtttcgg ggaagtaaga ataccggaca aagtgtaaaa
     1921 ccctgcccca tgagatatgg gtcaaactgg tcaccagctc cgaccgccag acatcagtaa
     1981 aagcagaaac gctctgattt tactgttgtc ctgcagtgaa cgtgcgttaa cttcaccgca
     2041 tgagcagtaa catagaaagg gaaacgtttc gcgcgaaacg gcgataagcg agagaatgta
     2101 aggagatggc gtgcatcggg caaactccgt acagagagga gaaaattcgc gcaatcatgg
     2161 cacctttcgc taaggcgtgc aagccaggac tttgcgacca gacaagccat caacaaggca
     2221 aaaagttcat aataagaaaa tcaaacaata cagctaacag gaactcttgt ggtcatcggc
     2281 ccttttatca acgctagtgc agtcttactg ggtggcgttc tcggcgcact gctcagccaa
     2341 cgcttaccgg aacgtatccg cgtctccatg acatctattt ttggtctggc atcgctgggg
     2401 attggtattt tactggttgt gaaatgcgcc aaccttccag cgatggtttt agctacgcta
     2461 cttggcgctc taattggcga aatttgtttg ctggagaaag gtgtcaatac agcagtcgcc
     2521 aaagcacaaa atctgtttcg tcactcacgt aagaagccag cgcatgaatc ttttattcag
     2581 aattatgtcg cgattattgt cctgttttgc gccagcggca ccgggatctt cggggcgatg
     2641 aacgaaggga tgaccggcga tccgagtatt ttaatcgcca agtcatttct tgatttcttt
     2701 acggcgatga tcttcgcctg ctcgctgggt attgcggtgt cggtaattag tatcccatta
     2761 ctgatcatcc agctaacgct agcgtgggct gccgcgctga tattaccgct gaccacaccg
     2821 tcgatgatgg cagacttcag cgccgtaggc ggtttattgc tgctggcaac cggattacgc
     2881 atctgtggca ttaaaatgtt cccggtggtc aacatgcttc cagcactcct tctggcaatg
     2941 ccgctttccg ccgcctggac cgcctggttt gcctgacaat gcgtgcaata gcggcaaagt
     3001 gatgatagat tgtgcagtct gcagtaaatt gaagaaattt gattgacgag acgaggcgaa
     3061 tcaggtttaa tgcgccccgt tgcccggata gctcagtcgg tagagcaggg gattgaaaat
     3121 ccccgtgtcc ttggttcgat tccgagtccg ggcaccaaat tcatatcaac ggacctccac
     3181 ggaggtccgt ttttcgtttc agaacgccac gatttaagcg ttctgcctac aaatcaattc
     3241 taccgaactc aaccagattc cccccacatc aaccccattg tgtgggtata attgcgggca
     3301 taccccagtt cgacagaatt tgtaccctct ttaacgcatt cagaaggctg aactatggca
     3361 ctgactgacg caaaaatccg ggctgcaaag cccactgaca aggcttataa actcactgac
     3421 ggggctggca tgttcctgct ggtacatcct aatggctccc gttactggcg tctccgttat
     3481 cgtattctgg gtaaggagaa gactctggca cttggtgtgt atccagaagt ttctctctcc
     3541 gaagctcgta caaaacggga tgaggcccga aaactgattt cggagggggt tgacccttgc
     3601 gaacagaaaa gagctaaaaa agtagtccct gatttacagc tctcttttga acatattgcg
     3661 cgacgctggc atgccagtaa taaacaatgg gcacaatcac acagcaataa agtactcaaa
     3721 agccttgaga ctcacgtttt cccctttatc ggcaaccggg atatcacaac actcaatacc
     3781 ccggatctgc ttatccctgt tcgtgctgca gaagctaaac aaatttatga aatcgccagt
     3841 cgtctgcagc aaagaatatc tgccgtaatg cgttatgccg tacagtctgg catcatcaga
     3901 tataatcctg ctctggatat ggctggcgca ttgactacgg taaaacgcca gcatcgcccc
     3961 gctcttgatc tttctcgcct gcctgaactt ttgtcgcgta ttggcagtta caaagggcaa
     4021 cctgtcaccc agcttgccgt tatgctgaat ttactggttt ttattcgttc cagtgaactc
     4081 agatacgccc gctggtctga aattgatatt gacaatgcca tgtggactat tccagccgaa
     4141 cgcgaacccc tgccaggcgt aaaattctca caccggggct ccaagatgcg aacaccacat
     4201 cttgtgccac tcagcaaaca ggctgtagcc atactgacag aactacagac atgggctggt
     4261 gaaaatggtc tgatctttac gggagcacat gacccgcgta aaccaatca
//
LOCUS       DAZPDU010000267         4264 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZPDU010000267 DAZPDU010000000
VERSION     DAZPDU010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4264
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.271"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..3514)
                     /locus_tag="ACHURS_004279"
     CDS             complement(<1..3514)
                     /locus_tag="ACHURS_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFR1239855.1"
                     /translation="MDENALGFTSYWRNSLADAESGKGSFERKDAKNFTHWHGIAAGR
                     LDEAIVSKFFKGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLIVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQGISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
     gene            complement(3966..>4264)
                     /locus_tag="ACHURS_004280"
     CDS             complement(3966..>4264)
                     /locus_tag="ACHURS_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1239856.1"
                     /translation="VNKALRVMGYDTTQDVCGHGFRAMACSALIESGLWSRDAVERQM
                     SHQERNGVRAAYIHKAEHLEERRLMLQWWADFLDANRDKGISPFEYAKINNPLK"
BASE COUNT         1101 a         1095 c          878 g         1190 t
ORIGIN      
        1 aaacctgacg cctctcaaga ctattataaa tagcctcaat ctcacccttc aggttatcac
       61 cgcaataaac cattgatgta tcgtatcgtt catatcgcgc ttcccgtgtc gcactaaacc
      121 agttgaacga tcccacacac agcaaaccat catcaccaat aacaattttg ctatgaacac
      181 ggtttaccag ctttgtagca atacccagcg cattcagttt ctccagcgcc gctttaaagt
      241 tctgctgctt ctcttttcgc ttctcaaaat cattatgttc agtgttgtag cttctgtcag
      301 tgactatcgt gacgttaatt ccacgtgaac acgccgcaat catggaatca agaaaaccgg
      361 tttgctccag cctttgccag gtcagccatg gagaaattat cgtgatgtgt ttactggtat
      421 tttcaaatgt ctgattcagg aaattatcat gttgctccac accatgaagt gtgtagattt
      481 tggtcccggc ggtttttaaa tccttacgct ctttataatc aaaagagagc gcattcttct
      541 ctgactcaaa gaggtatttt gccagtaatc cccgtggcga tgaggctggc tggacctcaa
      601 acaggtccat atcgccgaag accagaaaac tgtccttcgc acgggagact gcaacattca
      661 gcatgctgtt atcgctatca ataaacccgc cgtcttcatg ttttgaatag actggcgaga
      721 atatcacaat cgctctttcc gctccctgaa gagagtgcac ggtgcccact gtgagcgact
      781 tttcattcgt gcctgtactg ataccttgtt tgcccagcac ctgtttgata gtggatacct
      841 gagcgctaaa aggcgtcaca ataccgacaa cttcatgaag cgatttaccg taatgcgctt
      901 caatattttg ctggttctct gccaaccaga ccgctatcgt ttcagcctca agcaaattat
      961 atcgacttcc actacttgcc agctctcctt taccatcaat atggagatac cccattgcgg
     1021 gcattaaatt gctctcttca cgccctcttt taggcaacaa cttaccgtga tagcagagcg
     1081 tattacagta tccaataata ttgtcgtagc accggcggtg ttcatatagg tacataccac
     1141 gagccagttc gggatcatat tgatagcgcg aagcaaactg cgctattttc ataacgctgc
     1201 cagatgcggc acttttacca aggtctgcga ttgccgtata tttctcggta atctcttctt
     1261 gcgtactgcc agacagaatt ttttccgcca gcatgttacc gacatcaatc gcaggagcaa
     1321 tactccatat tggcgggatc tgctccgtat cgccaatcac taatgccttc ttagctaatg
     1381 caaacgaggc agcagccact tcaggaagca cctgcccggc ttcatcgaca atgagtaaat
     1441 cggcaaaatc atacaaataa cttttctcga atttacgttg tcctttgtgc tcacttatct
     1501 gcatattacc gggcagcata tagcatgtca ttaccacaca tggcgtgagt ttcattcgac
     1561 gttgccaacg ggcggttacc cctttagcac cttttttctt cttctcgtcc tgcagatcat
     1621 caatgctggc catatccatc agccaacgac cttcccagta gtgagtcgtc agtaaaaatg
     1681 cagggaagcg aatctgcgta tcagccagtt catcggcctg tgagaagctc agttcctcgt
     1741 cgccctcata ccctaaatca aatgccagcc tctgccactc ctgaaccgcc tgctgttctt
     1801 taagaacgat ttcatgggcg gagtcaatct gctgccggta tgttgtttgc tcgcgctcag
     1861 cggaattgag cagcccatca atattacgtt cgatagtttc aggatcagac cactgatttc
     1921 ctgcaatcag cgcgcctaat ttatcttcga gaaacagctg tatttggtac tgtcgcttat
     1981 tgcgaaccgc cgggagccag gaaaataatg aatagatcag tgattcaccg gcgcgatatt
     2041 ttttccattc cgttttagca ctcttcagta gagtgacttt ttgttcttgt ccggaaagta
     2101 atttatttaa attatcgaga tattgctcaa tatcattagc aataagctca cgcgcagccc
     2161 gaatctggct taacgtttgc catgttgcgt tcagtcttat cagttgctca gattttgctg
     2221 ccaactgacc atgcaggagt tcaatgacct tttcagggga tgaacactct tttccaggaa
     2281 aggctgcctt agccttttcc agataaaaca gcagtgcatc ctctacatac tcttttgact
     2341 caacctggtt gaagaaatct tcagtttgat attttttggc tgcctcagct ttacgactgc
     2401 ttgagggaaa ataagcaccg aagcttttca gctctggcaa ccatcgcccg gccatcgcac
     2461 ctgaaccttg cgaaaagtct ttcccgaatg cctcaataat gttcgttaca gcctggttat
     2521 tcgttgaagt cgcgataata accggaggct cagatttttc gagagccgct ctggcccact
     2581 gcgtggcgat gatagaaagc accagcgtgg tttttccggt tcccggaggg ccattaacag
     2641 caaggatatc gccatgtctt gcatccagaa aatggcttaa ggcatcgcgc tgagcctttg
     2701 ccagcggaaa cttatctccg gagtgtccaa gcctgtcgct gaattttgct cctggggcca
     2761 gcaaagactc tgcagcatga acctctcgcg aggcgaagcg attgaagagc ggcacatcct
     2821 tattgcaaac aatcaggtga tcataaagag aaaggatatg ggaactggca ccgccagatt
     2881 gagccgtttt aacaatataa ccgtgctcag cgagttcata ttgctcaggt ttttcaatcc
     2941 agtcgccggc aacgctcttc agtagcctct ctgagtcata cagatattga cgccactcct
     3001 gctgcaaggc ggcatatcgt gcttcccgtt cttcatccgt ttcggcagtc ttatcaacgc
     3061 tgtcatcaaa gttgatagag aacgtggtat gggtcgtctt gtatttgtca tactgcccaa
     3121 tctcaccaat cgaaaatgct ccttttggca aaggttcaag caggtctctg ggaatggagg
     3181 tcgctggcgt cggatataaa aaaccctcac ggcttagcaa ggctggcgtc actatcgggg
     3241 taacaatatc aggcgcacct gcagaacggt ccttaccatg ctgcagtaac cggaaataaa
     3301 cttttgggcg caagatgaca tcgaccgttt cgacatcgtc tttttctccc ttaaaaaatt
     3361 tactgacaat cgcttcgtca agacgtcccg ccgctatccc atgccagtga gtgaaatttt
     3421 tggcgtcttt ccgttcaaaa ctgccctttc ctgactcagc atccgcaagc gagttgcgcc
     3481 agtatgaggt aaaccctaaa gcattttcat ccattaccag tcctgtgact caatgaaatc
     3541 cattgattta tcgaatataa aaccaccata tggacaacta ttgtccatat tgaaaaagtt
     3601 ggatgcaatg ttttgtttat aattcggtat caaatgtact ggttgtacaa ggttcagaac
     3661 acaagcacat tcaacagtac gtcaggacta ttgatttcat aacagtgaat acatttttta
     3721 ccgtttataa tgactgcctg tgttggacaa ggataccaga acagccgaaa acaccggggg
     3781 ttattacctg ctctcatgga aaccagatat tcctggtgct aatcacagtc ataaatagcc
     3841 tgaagaacat gacgaaggga ctttaatgga agccggagta acttgccaga acatcaatct
     3901 tcgtcatcat ttacaaagaa aaataatcag ttaaagcagt ccgggcaatc agcccggact
     3961 gcttactatt ttaatggatt gttaatcttt gcatattcaa acgggctgat acccttatct
     4021 ctgttcgcat ccagaaaatc tgcccaccac tgtagcatca gtcgacgttc ttccagatgt
     4081 tctgctttat ggatatacgc agcacgtaca ccattacgct cctgatggct catctgacgt
     4141 tccacagcat cgcgggacca caaacctgat tcaatcaatg cactgcacgc catcgcccgg
     4201 aatccatggc cacagacatc ctgggttgtg tcatacccca tcaccctcag ggctttattt
     4261 acag
//
LOCUS       DAZPDU010000268         4075 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZPDU010000268 DAZPDU010000000
VERSION     DAZPDU010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.272"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..784
                     /locus_tag="ACHURS_004281"
     CDS             <1..784
                     /locus_tag="ACHURS_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR1239857.1"
                     /translation="AKTRHQINLSNSTSFSKDGYSSNNTGITGIAGEHDQLNYGIYVN
                     QQQQNNDTSLGTNLSWRTPIAIIDGSYSHSKNAWQSGGSISSGLVVWPGGINITNQLS
                     DTFAILDAPGLEGAHINGQKYNRTNSKGQVVYDLIIPHRENHLVLDIANSESETELQG
                     NRQIIAPYRGAVSYVQFTTDQRKPWYIQALRPDGSPLTFGYDVLDLQENNIGVVGQGS
                     RLFIRVDEIPTGIKVALNDEQNLFCTITFQHVIDENKTYICQ"
     gene            797..1756
                     /locus_tag="ACHURS_004282"
     CDS             797..1756
                     /locus_tag="ACHURS_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFR1239858.1"
                     /translation="MKWLLLITLSLYSFIVQSAPCALTNVGEQRGTYILKPLSMKGNL
                     TAEKIENTGNIIECADLQETTNAIKVHFLTQNALRFKVGSRHYIINFPFESDGKKIEL
                     PGRSSYNLEEMLSAINFTIPYSIASVSDSSNTADIFPGTPFPLLFEIEVSSCTGNNTN
                     CNSVRFNYNLNTILQVNLMTCGFEDQSIDTGRFVLSRINNENYQFHHIRFDCIQGEQG
                     ELVFEPSDVEYYFEPVTIQESGTSILKNELENSEDGAGQVGFQLSNDGTNEIQYGKSN
                     YYSFQHPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
     gene            complement(1794..2693)
                     /gene="nhaR"
                     /locus_tag="ACHURS_004283"
     CDS             complement(1794..2693)
                     /gene="nhaR"
                     /locus_tag="ACHURS_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFR1239859.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKSFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(2759..3925)
                     /gene="nhaA"
                     /locus_tag="ACHURS_004284"
     CDS             complement(2759..3925)
                     /gene="nhaA"
                     /locus_tag="ACHURS_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFR1239860.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
BASE COUNT         1224 a          952 c          861 g         1038 t
ORIGIN      
        1 tgccaaaaca cgtcaccaaa ttaacttatc gaattcgacc tcattttcca aagatggcta
       61 ttcctccaac aatactggaa ttactggcat agccggtgaa catgatcagt taaattatgg
      121 tatatatgtt aatcagcaac aacaaaataa tgatacctcg cttggtacga atttaagctg
      181 gagaactccc atcgccataa tagatggcag ctatagtcat tctaaaaacg cctggcaaag
      241 tggtggaagt attagtagtg gattagttgt ctggcccggt ggtattaata tcactaacca
      301 gttatccgat acatttgcaa ttctggatgc gcctgggtta gaaggtgcac atattaatgg
      361 acaaaaatac aatcgaacaa acagcaaagg ccaggttgtt tacgacctga taatacctca
      421 tcgtgaaaac catctggtac ttgatatagc aaacagtgaa agtgaaacag aattgcaggg
      481 caatcgtcaa attattgcac cttaccgtgg agcagtttct tatgtgcagt ttacaactga
      541 ccaacgtaag ccctggtata tacaggcact gcgtcccgat ggttcgccat taacctttgg
      601 ctatgacgta ctggatctcc aggaaaataa tattggagtc gttggccagg gtagtcgcct
      661 ttttattcgc gtagatgaaa ttcccactgg aataaaagtt gctctcaatg atgaacagaa
      721 cttattctgt actattactt ttcaacacgt tatcgatgaa aacaaaacat atatatgcca
      781 gtaaaaggag tttactatga agtggctttt attaatcacc ttgagtctat attcttttat
      841 tgtgcaaagt gctccctgtg cattgaccaa tgtcggggaa caacgtggaa catatatact
      901 caagccactg agtatgaaag gtaatctcac cgccgaaaaa attgaaaaca ccggaaatat
      961 cattgagtgt gcagacttac aagagacgac caacgccatt aaagttcatt ttttaacaca
     1021 gaacgcttta aggtttaaag tcggaagtcg tcattatatt attaatttcc cctttgaatc
     1081 tgacggtaaa aaaatagagc ttccaggaag gtcatcttat aatttagaag aaatgctatc
     1141 tgcgataaat ttcaccattc catactctat cgcatctgta tcagatagtt ccaacaccgc
     1201 agatattttt ccaggaactc cttttccttt gttgttcgaa attgaagtga gttcatgtac
     1261 agggaataat actaattgta atagcgtccg ctttaattat aatttaaata ctattttaca
     1321 ggtaaattta atgacctgtg gttttgaaga tcaatccatc gacactggac gatttgtact
     1381 gtctcgtatc aataatgaaa attatcagtt tcatcatata agatttgact gcatccaggg
     1441 cgagcaaggt gaattagttt ttgaacccag tgatgttgaa tattactttg aaccagtgac
     1501 gatacaggag tcaggaacca gcatattaaa aaatgagtta gaaaacagtg aggacggtgc
     1561 gggtcaagta ggctttcaac tctccaatga tggtacgaat gaaatccagt atggtaaaag
     1621 caattactac tcttttcagc atccccatga aggaagtaat cagattcctc tatttattcg
     1681 cccgcgtaca tatggaaata atgtctcaag cgggcaaata atgagccgag tcaaaatcgt
     1741 ggttatgtac aactaatcta tctcccccac cttaactttg ggggaggtgc cgattaacgc
     1801 gccgctggac taaaaagcgc agaataatcc gtattgcaga ttcgctgtac cgccgggtgc
     1861 tgaatcatcc gctcagcaaa aatagcatgg tactcttcca tcacattctc gacgcgacca
     1921 atttctacga cagttttatc ggcataaaag tcatatgcat aaagcgttgg ggcaacgaag
     1981 attgcattgt gcatcgcacc aaaagctttc atcaacgcgg catcatcaaa ctcgccgagg
     2041 atttctacgt ttaatccctg ggagttaaac cagttaagca atttgcgccc taacattgaa
     2101 cgtcgcccag gaatcaaaag tcgccgttct tccagacaag ccgggaacga tttttctggt
     2161 ggtggatttg tacaccagaa actcacgcca cattcgccaa ttctcacgga gaacaggcct
     2221 tcctgctgcg tagagtctat cggacagtca gaaatgatca tatccagttt atgctgactt
     2281 aattgctcca gcagcatttc gtgggtggat tcgaagcagc gaagatgaat gggctcgcct
     2341 tctaccactg cggcgttaag tacgctactg accaggcgtt tggaaagtgc atcagccacg
     2401 ccaacgtcaa acaataaatt ggattctttg cgatagttca caatatccag catttcctgg
     2461 cttaaggtga acattttatc ggcatagcga tagaccagtt ctcccagctc gctgggttcg
     2521 agaccacgtc ccttgcgttt aaataatttg ccttgcaggc gctcttccag cgctcgaatc
     2581 tgtccggtaa tggtttgtgg agttaaataa agcgcctccg ctgcgccaac cacggaacct
     2641 tctttataga catgccagaa gtaatacaag tggttgtaat tgatatgaga catgctcatt
     2701 tctctccctg ataacaatga aaagggagcc gtttatggct ccccggtaaa ccgtcctgtc
     2761 aaactgatgg acgcaaacga acgcgtaacc agctgtatcc aattaccgcc gaagagatag
     2821 aaccgaccag gataccgagt ttcgcccagt taatcagttc tggatctacg ctaccaaagg
     2881 ccaggctggc aataaagata gacatagtaa aaccgatacc gcacaggatc cccaccgcca
     2941 taatttgctg ataagtcgtt ccctcaggca gatgcgccag tttcaaacgc agcgccaacc
     3001 agcagaacag actaatcccc agcggtttgc caatcagcaa gccagcgatg atccccaatg
     3061 gcagaatgga ggtcaagcca tccagcgtga cgccttgcag tgaaacgcca gcattagcaa
     3121 atgcaaacag cggtaaaatc agatacgcca cccacgggtg caatacatgc tccagtcgct
     3181 tcgctggaga acgcccatgc ttctctttca aaggaataaa gaagccgaca attacccccg
     3241 ccagggttgc gtgaaccccc gatttcaaca ccgcagtcca caacaccacg ccaacaagaa
     3301 tatagacgcc cgtgcggcgt acaccacaca gattcaatac cgcgagtacc gcaattgcta
     3361 cagccgcgac gccaagagag gccatcgata agtcattagt gtagaacaat gcgatgataa
     3421 tgatggcccc aagatcgtcg ataatagcca gagccatcaa aaagatcttc agtgctaacg
     3481 gaacacgact tcccaacagc gccagcacac caagtgcaaa ggcaatgtca gtagccgccg
     3541 ggatcgccca cccttcgcgg gtaatcggat cggcatagtt aaaggccaga tagagtaatg
     3601 ccggaacaat catcccacca atagcggcga taaccggaaa tgcggcctgg cgtaagctgg
     3661 ctagcgatcc ttgcatcagt tcacgtttaa cttccagacc gactaacagg aaaaataccg
     3721 ccatcagcgc gtcatttatc cataacagca tgtttttgtt gatttcgagt gaaccaaccc
     3781 ggagctgaac cggcgtctcc agaaagtcgt gataccatcc actggttgcg ccgctgttgg
     3841 ccatcatcat cgccaggata gcggcaatga taagaataat gcctcccgag gcatcactgc
     3901 taaagaatcg atgcagatgt ttcacttttt atttctcttt caggtgaata gatcgttttt
     3961 actattttac cccgcacaaa tcatcgttaa aatcagatta tagtgtagga ataactcgtt
     4021 ttttacgagc aattaaagga tttgcgccat cagcggtgag atgagttttt gacgg
//
LOCUS       DAZPDU010000269         4072 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZPDU010000269 DAZPDU010000000
VERSION     DAZPDU010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.273"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            75..776
                     /locus_tag="ACHURS_004285"
     CDS             75..776
                     /locus_tag="ACHURS_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR1239861.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPD
                     EQKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRA
                     QYFKGEAKVDITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTG
                     DRTIAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVA
                     LHFRFKEDKQIKMTI"
     gene            complement(1034..3181)
                     /gene="mobQ"
                     /locus_tag="ACHURS_004286"
     CDS             complement(1034..3181)
                     /gene="mobQ"
                     /locus_tag="ACHURS_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="HFR1239862.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTR
                     KSGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAAR
                     ETVLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAIGPAGFSGKVRDWNDRTH
                     AETWRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETE
                     RGEQNRLINRLNLEIQISRTELRNGGLTEPARRVADLLNIGLPDNATSYTLRDIIEAL
                     PKDSNSAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRI
                     PLMQWAAPEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQMAFEKQWQQWGESGL
                     AALRAQLLKREEELRRKEQEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGN
                     GEMTCYLLLHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQRPPLQR
                     VYSSATFEQLLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSPAARKVDFMQG
                     RLPPTLMPVMSSVDRETRQLQLLLVMGVDDSLGGVIRLNGTLYPAFAVPSADNRHLVI
                     SALTDKGLRYAGYGVAVNHDADSHISPAPELMQFHLKTREAPLFAAVNTPEKQPDHLF
                     RSLGFNRTWEEWQREEDARTHTTERRHDRGWSQ"
     gene            3360..3614
                     /gene="mobC"
                     /locus_tag="ACHURS_004287"
     CDS             3360..3614
                     /gene="mobC"
                     /locus_tag="ACHURS_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="HFR1239863.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRK
                     PEEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
BASE COUNT          962 a         1030 c         1066 g         1014 t
ORIGIN      
        1 aaaaaaaaaa cttccaccca tatgataagt ggcgtacagt aaaaaaacgc caccaatagc
       61 aacgagcaag cgaaatgtca gatctgatag cttacaaatc aaacgccctt atcgaggcaa
      121 gctacaagct cactttgcag gaacagcgct tcctgctggt ctgtatcagc cgcctgaaat
      181 ccggagaaga ttccccttcg ccggatgaac aaaaaaccat gaccatcacg gctgcagagt
      241 ttcatgatgc ctttcctgac atgggacggc agcacgctga agctaggctc aaggaagcga
      301 tagatcgcct ctgggacagg tcagtaatca tcaagaacga ggaaaaacga gaagagttcc
      361 gctgggtgca gtaccgggcg cagtatttca aaggtgaggc gaaagtagat atcaccttct
      421 ccgatgccat catgccgtat ctgacacagc tcaaggggca gtttacgcgg gtagtcgtga
      481 agaacgtctc cagcctttcc agcacctaca gcatccgcat ttacgagctg ctccagcagt
      541 tccgcagcac cggtgacaga acgatagcta tcgatgattt ccgaaccatg cttgccgttg
      601 aagataaata tcaacaattc aaagacttga ataagatgat aatcaagcca gccatcgccg
      661 aactgaacga aaaaagcgat ctggttgtaa ctgttgagac gatcaaaaag gggcgcacgg
      721 ttgtagccct gcattttcgc ttcaaggaag acaaacagat caagatgacg atctaaaatc
      781 gattttaagg cacctaaaac gctccctgct gcgttttttt tgtgttagta gtgcgaaggt
      841 actattgaag tgaaaaattg cgtcaaaaac gctctgatgc agcgaaaaaa gaccccccaa
      901 tttttggggg cggaaaaaca aaactatgct tgcgttatgt gagtgtgctt cttgcattca
      961 tccctgtcga tgaacatcga agaacatcga cgaacataac acgatgttaa acaaaacaaa
     1021 atattgttca cattcactgt gaccaaccgc gatcatgtct cctctcggtg gtgtgtgttc
     1081 gtgcatcttc ctcgcgctgc cattcctccc aggtacgatt aaaacccagc gagcggaaca
     1141 gatgatccgg ctgtttttct ggtgtgttga cggcagcaaa aagcggtgcc tcacgggttt
     1201 tcagatgaaa ctgcatcagt tctggtgcag gtgatatgtg gctgtctgca tcgtgattta
     1261 cggcaacacc ataccctgca taccgtaacc ccttatcggt cagcgcactg atgaccagat
     1321 gcctgttatc ggctgaggga acggcaaacg cgggatacag cgtaccatta agccggataa
     1381 caccgcccag agagtcatcc acgcccatga ccagcaaaag ctgaagctgg cgggtttccc
     1441 ggtcgacaga actcatgacc ggcatcagcg tcggtggcag acgtccctgc ataaaatcca
     1501 ccttccttgc cgctggtgat ggcatggctg gctggcggtc ttctccaaga cagaaagact
     1561 ccggccatgt cttcggctgg cggagattgc gggtatgttg cggccatgtc tggcaaacct
     1621 gcgtcagcag ttgctcaaag gttgcagagg aataaacacg ttgcagtggc ggacgttggc
     1681 cgatttcgct tcggtcacag atataaccac tcctgtcacg cattaaatca acactctcaa
     1741 agattctggt gcggtatttc tccagctcgt tcccccagag cgtatattct ccgttccggt
     1801 tgtgaagcag aaggtaacag gtcatttccc cgttaccagg catgggagcc tcgccatacc
     1861 ccgtaacgac tgctctcagc acccctgaat ccataacggc attgtcattt ttcctgagct
     1921 gctccgcgcg actacgttca gtatcttcct gttccttccg gcgcaattcc tcttcacgtt
     1981 taagcaactg ggctctgaga gctgcaaggc cggattcccc ccactgctgc cactgttttt
     2041 caaatgccat ctgactcgcg ggaatgtcct gtttctccac cttccggtaa tgctgtcgta
     2101 atttttccat ttgctggctc agagactgga tttttccctg ctcactacgg tattccggtg
     2161 ccgcccattg catcagggga atacgggaaa aaccggacga aaacggcgat gattttttca
     2221 ggctggcagc atggctcatc gcttcctttc gttcggtgtt cagctcttcc cagcgtctgc
     2281 gcgtcgcctg catatctgcc atcatcgaca tggcattact ggtgagtttc catgccgagt
     2341 tactgtcctt cggcaacgct tcgataatgt cacgtagtgt gtacgaagtt gcgttgtcag
     2401 gaagcccgat attcagaagg tcagccacac ggcgggcagg ctctgtcagc cctccgttcc
     2461 tgagttcggt gcgggaaatc tgtatttcca ggttaagcct gttaatcaga cgattctgtt
     2521 cgccgcgttc tgtttcgata ccgcgtgttt ccatcgcaca ggcagacttt ccgaggtgaa
     2581 taccaggcgt tttctcaagc ccctgtcgct cgtatgaacg atggtctatc tcttcctgat
     2641 aaccggcgtt cgccagtgcc ctgtttgcat ggtcagccca tgatgcccgc cacgtctcgg
     2701 catgtgtccg gtcgttccag tccctgacct ttccgctgaa cccagcaggt ccgatagccc
     2761 tcgtggtcag catgatgtgc gcatggggat ttgttttatc catgtgatgg aacgccacat
     2821 cagcaatcat gccctgactg acaaagtttt ctctgacaaa ggcgagaacg gtttcccgtg
     2881 cggcatcctg cggcagttcg tgggggatgg cgagttcaat ttcccgcgca agttgtgagt
     2941 tcttccggcg ttcggccttc tcaacggcat tccagagttc ggcacggttc gtacaccagg
     3001 caggggcggt ggcgggggtc agaatcacag cctcagccac accgcttttg cgggtgtagt
     3061 cgtgagtcag tccggtacgc tcatcaagca tcagtgaacc ggaacgatag gcagcggaag
     3121 ctacggcgct gtagccgctg cttctggaga taattttcat gctgagatga taaatcgcca
     3181 tttctgccgt aatgcctttt tgtagttttc tttcagcccc gcagggcgca cacaatcgaa
     3241 acggagtgga gattgtataa gtgcgcactt cgtgatttgc cgtgcattat gccagctcag
     3301 tttttaaact accagtgacg cgctaaaatg cgatttttca tcgcgcacgg agttacatca
     3361 tgagcagaac gctcgaacag aagattgctg atgctgaggc gagattacag cgcctgaagg
     3421 cgaagagccg gagcctggac acagcgcaga aggttgttgt gggtgctgca ttgctggcaa
     3481 aggtcagaaa gccggaggag gtgcagttgc gtgcctggtt actccagttc ctgaaggcag
     3541 aggtgacacg acaggctgat gtgacgcgca tactgccgct gattaacgag ctggaggcgc
     3601 tgccagaaca gtgattgtat cgtccgctcg ccgcagcccc agcgaccgag cgttagcgag
     3661 cgagtgggcg aggaagcgga agtgaatcag tcgccaggtt gagcacatac gcacggtttt
     3721 tcggcaggtg atatgcgctg aacccgcatg aatactgggt ttccacaggg ttggaggggg
     3781 tgtattcgaa acttgcaatt ctgctttatt tcaacgcatt gtttttattg gttttttgtt
     3841 ttcagcaccc caagttatcc acaggctcgc cgttaagttt ttaagatctt atttttagat
     3901 ctttttttta aaaccttttt aggtcttgta acaccatgat tttaaagatg ttctggcaag
     3961 ataagtggag cttttggggg tgaaaggtgg agcttttggg ggtgaaaggt ggagcttttg
     4021 ggggtgaaag gtggagcttt tgggggtgta atccacagaa aaagggggtt gc
//
LOCUS       DAZPDU010000270         4049 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZPDU010000270 DAZPDU010000000
VERSION     DAZPDU010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4049
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.274"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            30..758
                     /gene="mpaA"
                     /locus_tag="ACHURS_004288"
     CDS             30..758
                     /gene="mpaA"
                     /locus_tag="ACHURS_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFR1239864.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(733..1698)
                     /gene="ycjG"
                     /locus_tag="ACHURS_004289"
     CDS             complement(733..1698)
                     /gene="ycjG"
                     /locus_tag="ACHURS_004289"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFR1239865.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVSQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKIGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            1817..2323
                     /gene="tpx"
                     /locus_tag="ACHURS_004290"
     CDS             1817..2323
                     /gene="tpx"
                     /locus_tag="ACHURS_004290"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFR1239866.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTDVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(2367..3908)
                     /gene="tyrR"
                     /locus_tag="ACHURS_004291"
     CDS             complement(2367..3908)
                     /gene="tyrR"
                     /locus_tag="ACHURS_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFR1239867.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICVTQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVQRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
BASE COUNT          970 a         1142 c          972 g          965 t
ORIGIN      
        1 ctgtaatact tcctgaacca gagacgatca tgaccgtaac ccgcccacgc gccgaacgcg
       61 gcgcatttcc gcccggaaca gaacattacg gacgttcatt attgggcgcg ccgttaatct
      121 ggtttcctgc ccctgcagcc agccgcgaaa gtggtttgat tctggctggc actcacggtg
      181 atgaaaactc ttcggtcgtc acgctctcct gtgctctgcg gacgttgaca ccttctttgc
      241 gccgccatca tgtggtgctg tgtgtgaatc ctgacggctg ccagctgggg ttacgggcca
      301 atgctaatgg tgtggattta aacagaaact ttccggcggc gaactggaag gaaggtgaaa
      361 cggtttatcg ctggaacagc gccgctgaag aacgtgatgt cgtattactg acaggtgaca
      421 aacctggctc agaacctgaa acccaggcgc tgtgccagct tatacaccgc attcaacctg
      481 cctgggtggt ctcattccac gatccactgg cctgtattga agatcccaga cacagcgaat
      541 taggcgaatg gctggcccag gcgtttgaat tgcctctggt aaccagcgtc ggttatgaaa
      601 cgcctggctc tttcggcagt tggtgtgcag atctcaactt gcattgcatc accgcagagt
      661 ttccgcccat ctcctccgac gaagccagcg aaaaatatct ctttgcgatg gcaaacttgc
      721 tgcgctggca tcctaaagat gcaattcgcc cgtcgtgaac tgaagcgccg gttccacatc
      781 taccgccagc caggtcggtc cgtcaagatc ggcgaaactg acctgcggca ccagcggtaa
      841 agcggcgcta atggcacgag aggtacacaa catgcagccc agcatcagac tgaaaccttg
      901 tgcacgcgct tcagtcgcca gcgccagcgc ttccgtcaga ccaccgattt tatcgagctt
      961 aatgttaacc atctcatagc gccctttcag cgccttcaga ttgctacgtg tatgacaact
     1021 ctcatcagca caaatcggca acggatgaat aaaattctcc agcgccgcat cgtcctgcgc
     1081 aggaaggggt tgttcaagca tcgcaacgcc taaatccgcc aatagctggc aacgcgccgc
     1141 caacccttct gcacgccagg attcatttgc atcaacgatc agcgtcgcat cgggcacagc
     1201 tgtgcgaatt gccaccatcc gctcactaat aagatggtta tccagcttca ctttcagtaa
     1261 tttcgcgcct gcctgccaga gtgttgatgc actattggcc atctgatcag gcgtaccgat
     1321 gactaccgtc tgtgcagtga taactgtctc gggaagcgtt atgccgatca aatcagccag
     1381 cgattgctgc tgttttcgcg cggccagatc ccacaatgca caatccagcg cattacgtgc
     1441 tgcgccggca gggagaattt tttgcaactc ctcccgtgtc agccctttct ctaattgcga
     1501 cacgacgctc ataatttgcg ccattaccga ggcatcactt tctccataac gcggatacgg
     1561 cgtgcattcg ccggtgcctt taataccctc ttcttccagt tcaaccacca ccacgcgcgc
     1621 ttcactgcga cttccccggg caatcacaaa cggggtatgt aagggccagg cttcctcaaa
     1681 taccttaacg gttctcatct ttactccttg ttgtcgcggt ggtctgcgga ttaagcgcaa
     1741 atagcgtttg ctgtgttatt gacagttagc ataaactagg tgtgacgtta actatatgta
     1801 aacaggaaag ataatcatgt cacaaaccgt tcatttccag ggcaacccgg ttacagtcgc
     1861 caattccatc ccgcaggcgg gtagcaaagc gcagactttt actctcgtgg caaaagatct
     1921 gtctgacgtc accctcggtc agtttgcggg taaacgcaaa gtgctgaaca ttttcccgag
     1981 tattgatacc gatgtttgcg ccgcatcagt acgcaagttt aaccaactgg caactgagat
     2041 cgacaacacc gttgtgctgt gtatctctgc cgatctgccg ttcgcccagt ctcgtttctg
     2101 cggcgcagaa ggtctgaaca acgttatcac cctctccact ttccgtaacg ctgaatttct
     2161 gcaagcttac ggtgtggcaa ttgctgatgg cccactgaaa ggtctggcag cgcgtgccgt
     2221 tgtggttatt gacgaaaatg acaatgtgat tttcagccag ctggtggatg aaatcaccac
     2281 cgagccggat tacgaagcag ctctggctgt actgaaagca taagttaata tgcctgatgg
     2341 tgttgcacca tcaggcatat tcgcgcttac tcttcgttct tcttctgact cagaccatat
     2401 tcccgcaact tattggcaat cgcggtatgt gaaacgccga gacgttttgc cagtttgcgc
     2461 gtgctgggat aattgcgata aagctgggtt aataccgagc gttcaaaacg gctggtgatt
     2521 tcgtccagcg aaccttccat cgcatcttcg cccacggcta ccgttgcggc gtcataatcc
     2581 ggcaacaaaa tatcctgtgg acgcagctca taaccgtcca gttgtgtcag tgcgcgatag
     2641 atagcgttct ttaactgccg cacatttccc ggccacgcat aacgcgtaag tacagtattc
     2701 aggtcagcgg ccagtttcgg acgctgcacg ccctgctcgt cggcaaagcg ggcgacgaac
     2761 agctcagtta acggcatgat gtcctgcgga cagtcacgta gcggcggcag attgagcgtc
     2821 aacacgttca gacgataata gagatcttca cggaacatgc ctttttgcac cagttcgacc
     2881 agattcttct gcgtaacgca aatcacccgc acatcgacat gcacctcatg gtcttcgcca
     2941 acccgacgga aagtgccatc attaaggaaa cgcagtaatt tcgcctgcat ccgtggtgac
     3001 atttccccta tttcatccaa cagcaccgaa ccaccgttcg cctgctcaaa gaatcctttc
     3061 ttcccttccg gagcatgacc aaacagttca ctctcgaccg catcttccgg tatagacgca
     3121 cagttcagcg ccaggtaagg tttgcccgct ctggggcttg cctgatggca ggcgtaggca
     3181 aagagatctt tacctgtacc tgtgtcaccc gtaatcagca gcggcgcgct tagcatcgcc
     3241 agtttctgcg cctgttcgac aacatgcttc attttcgggc tgacggcgac aatttgactg
     3301 aaggcgctga cgtcctgggc ggcgacattt tgcaactggc ggcccatacg aatcgttgat
     3361 cgcaacatca ccaccgcacc ggtcaggacg tgttgatcat tttcatcctg aagataaaca
     3421 ggcgtaatct ccatcaggaa attctgccca ttaataacga catgctcgtt atgcgaatct
     3481 tgcggttcgc tttccagcca acgtaaaaaa ttaaagccgt taatcaattg tgcggcggta
     3541 tggttgcgca ggcgatccaa tttttgccca aaaagctgac agctcgccgg gttcgccata
     3601 tccactttgc ttttcatatc gacagagagc acaggttcag gcaacgcctc cagtaacgcg
     3661 ctcaacgcca gatgctcacg ttcggaaggc atccacggga cagtacgcac atcggtaaca
     3721 cccgcaatac ggcgtatttc ggccatcaga ctgctgaaac tctcaaactc cagttcagca
     3781 aaattgaggt agattcgccc aatgggatca atctcaatac cgcgtaaatc aatgcctctt
     3841 agcacgagta gatcgagtaa ttcgcgggtc agaccgagtc ggtcttcaca aaagacttcc
     3901 agacgcatgg gaaccttcac ctgaaaaaag aacaattaat atgatgatat gacactattt
     3961 ggatagcagg aaggtttctg tcaacaatca ttgacacaaa gacggaaaaa cgggcacttc
     4021 ggcgtaaaga tatccggtca gcacccgat
//
LOCUS       DAZPDU010000271         4043 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZPDU010000271 DAZPDU010000000
VERSION     DAZPDU010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.275"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            926..1642
                     /gene="nanC"
                     /locus_tag="ACHURS_004292"
     CDS             926..1642
                     /gene="nanC"
                     /locus_tag="ACHURS_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFR1239868.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            1662..2768
                     /gene="nanM"
                     /locus_tag="ACHURS_004293"
     CDS             1662..2768
                     /gene="nanM"
                     /locus_tag="ACHURS_004293"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFR1239869.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSLAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            2833..3813
                     /locus_tag="ACHURS_004294"
     CDS             2833..3813
                     /locus_tag="ACHURS_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFR1239870.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPNNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
     gene            3923..4043
                     /locus_tag="ACHURS_004295"
                     /pseudo
     CDS             3923..4043
                     /locus_tag="ACHURS_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
BASE COUNT         1219 a          845 c          915 g         1064 t
ORIGIN      
        1 ggagataagg gggaacgcag gagacgcttt ttcatctaca ttcgtgggta aatatggctt
       61 tggtcactgc gacttaaacc ttcctcaatc acagctattc gccgggcgat acggcaaaca
      121 aactaagtga aacatcatcc tgaaaacacc aacatcaaca agcctctcta gatcgacttc
      181 agaagcgacc agttacaagc cacaaatcag gaaacatatt tcatggtgag aattatgatc
      241 agcctgatgg cgggtaaaca cttgttttca ccacgctcac tgagttagct tgcaaagctt
      301 cccttgttta gaccctaaat aaatttttta taaaacaaac agttaaaaga aatcctttag
      361 tttttgaaga ccacctcaag tgttcgtctg gcttcacatg gcatcttcct cttagaaaaa
      421 gatcgacata ttttgtgaca cgaattgcaa atctcgtttt gttgtatggc ttgcgtgatt
      481 tttgatctgg tataacaggt ataaaggtgc accgcgatag tcaatgagac agggcatctc
      541 gcaatctatg gcaaacatca cttcagttct ttctcatcgg gtgatgaaaa cgcacttcag
      601 tctgaaagga atatgaaaat gagatcaaca gacactctat tttatgactc tgggtaaaat
      661 ggattgagta agtgatatag cttacgaaca ttcaaatcaa ttaaacatca gaagagattt
      721 tatactcagg tatttaatct gattctctgt ttatttaaat aatgtggaaa gagaattttc
      781 acaggagacc ttatacaaaa atataaaata agctacccgt tgccaaagac actataagcc
      841 tggcaaaaaa tattacacaa cataaatgct aattgtttat gcgggctttg tattgctttc
      901 tgtatcctac aaatgagtga aatttatgaa aaaggctaaa atactttctg gcgtattatt
      961 actgtgcttt tcgtcaccat taatttctca ggctgcgaca ctggacgtac gtggtggata
     1021 tcgtagcgga agccatgcct atgagactcg actcaaagtc agtgagggat ggcaaaatgg
     1081 atggtgggca agcatggaaa gtaatacctg gaataccatt catgataata aaaaggaaaa
     1141 tgccgcactc aatgatgttc aggttgaagt taattacgcg attaaacttg atgatcaatg
     1201 gacggtgcgc ccgggaatgt taacgcattt tagcagcaac ggcacacgct acggacccta
     1261 cgtaaaactg tcctgggacg cgacaaaaga tcttaatttt ggcattcgct atcgttacga
     1321 ctggaaagct taccgacaac aagacttatc cggtgatatg tctcgtgata acgttcatcg
     1381 ttgggatgga tatgtcactt accatattaa tagtgatttc accttcgcat ggcaaacgac
     1441 gctatacagc aaacagaatg attatcgcta tgcaaaccac aagaaatggg cgacggaaaa
     1501 tgcctttgtt ctacaatacc atatgacgcc cgatattacg ccatacatag aatatgacta
     1561 ccttgaccgc cagggtgttt acaacggcag agataattta tcggaaaaca gttatcgcat
     1621 tggtgtgtca tttaaactgt agtagacagg agacagtcac aatgaataaa actataatgg
     1681 cgcttgctat catgatggct tcatttgccg caaacgcgtc tgtattaccg gaaactcctg
     1741 tgccatttaa aagtggtacc ggagcaattg ataacgacac tgtctacatt ggtttaggta
     1801 gcgcaggtac agcatggtac aagctagata cacaggccaa agataaaaaa tggactgcgt
     1861 tagctgcatt ccctggtgga ccgagagatc aagcaacctc tgcatttatt gatggcaatc
     1921 tatatgtgtt tggcggcatt ggcaaaaaca gcgagggatt gactcaggta tttaatgacg
     1981 tacacaaata caaccccaaa accaatagct gggttaaatt gatgtcgctc gcgccgatgg
     2041 gcatggcggg tcatgtgact tttgtacaca acggcaaggc ttatgttact ggcggtgtta
     2101 accagaatat cttcaatggc tattttgaag atctcaacga ggctggaaaa gattcaaccg
     2161 ctatagataa aatcaacgcc cactattttg acaaaaaagc agaagattat ttcttcaata
     2221 agtttctgtt gtcttttgat ccctcaacac agcaatggag ttacgctggc gaatcgccct
     2281 ggtacggaac ggctggtgcg gcggttgtga ataaaggtga taaaacctgg cttattaatg
     2341 gcgaagccaa accaggattg cgaacggatg ccgtatttga acttgatttc accggtaata
     2401 atttaaaatg gaataagctt gatcccgtct catcaccaga tggcgtcgct ggcggttttg
     2461 cggggataag caatgattct cttatatttg ccggaggggc cggattcaaa ggttcacgag
     2521 aaaattacca gaacggtaag aactatgcgc atgaaggcct gaaaaaatca tatagcactg
     2581 atattcatct ttggcataac gggaaatggg ataaatcggg tgaattatcg caaggtcggg
     2641 cctacggagt atcattgccc tggaataata gtttattaat tattggcggt gaaactgcag
     2701 gcggcaaagc ggtgacggat tcagttttaa tttctgtgaa ggataataaa gtcacagtac
     2761 aaaactaacg cttcagggcc ccggtaaagg ggccctgcta tcaatttgtc atttgtatta
     2821 cgcgaaaatt atatgaacgc aataatatcg cccgattatt actatgttct taccgttgct
     2881 ggtcagtcta atgccatggc gtatggcgaa ggactgccat taccggacag ggaagatgcg
     2941 cctcattcca gaattaaaca attagcgaga tttgcgcata cgcatcccgg aggcccgcca
     3001 tgtcacttta acgacattat tccactgact cactgcccac acgatgttca ggatatgcag
     3061 ggttatcacc atcctctggc aacgaatcat caaacacagt acggcaccgt tggccaggca
     3121 ctgcatattg cacggaaatt actgcccttc attcctaata atgcagggat tcttatcgtt
     3181 ccgtgttgcc gtggcggatc ggcttttacc gcgggcagcg aagggacata ttcagaacgg
     3241 cacggagcca gccatgatgc ttgtcgttgg ggaacggata ctccgctgta ccaggattta
     3301 gtcagcagaa cgcgagccgc actggcaaaa aatccgcaga acaaattcct cggcgtatgc
     3361 tggatgcaag gcgaatttga cttaatgacc agtgactacg cgtcacaccc tcaacacttt
     3421 aatcatatgg ttgaagcctt tcgtagggat ctaaaacaat accattctca gcttaataat
     3481 attactgacg cgccatggtt ttgcggcgat accacctggt actggaaaga aaatttcccg
     3541 cattcgtatg aagctattta tggcaattat caaaataatg ttttagccaa tattattttc
     3601 gtcgacttcc agcaacaagg tgaaagagga ctgacgaacg cgcctgatga agacccggac
     3661 gatttaagca cgggatatta cggttcagcg taccggtcac cggagaactg gacgacggca
     3721 ctgcgaagca gtcatttcag cacggcagcc cgtcggggga ttatttctga cagatttgta
     3781 gaagcaattt tgcagttttg gcgcgaaagg tgagcgggga gtttattcat gggggatgtc
     3841 cttgttcgtt ctcagagtag acaacaaaaa acaaataaga tcaaggtaat atctcttcac
     3901 ttgcgggctc gaacagcagg caattgccaa tataataatc tggcacaagg gtgaaaaacg
     3961 cgcaccgcat aaaccgttgt tattgcttac agattagccg gatgccttaa tagacatccg
     4021 cgccttttcg attatggctc gga
//
LOCUS       DAZPDU010000272         4041 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZPDU010000272 DAZPDU010000000
VERSION     DAZPDU010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4041
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.276"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            376..483
                     /locus_tag="ACHURS_004296"
     CDS             376..483
                     /locus_tag="ACHURS_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR1239871.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(570..2249)
                     /gene="bcsG"
                     /locus_tag="ACHURS_004297"
     CDS             complement(570..2249)
                     /gene="bcsG"
                     /locus_tag="ACHURS_004297"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFR1239872.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFATFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGASVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(2246..2437)
                     /gene="bcsF"
                     /locus_tag="ACHURS_004298"
     CDS             complement(2246..2437)
                     /gene="bcsF"
                     /locus_tag="ACHURS_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFR1239873.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(2434..4005)
                     /gene="bcsE"
                     /locus_tag="ACHURS_004299"
     CDS             complement(2434..4005)
                     /gene="bcsE"
                     /locus_tag="ACHURS_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFR1239874.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
BASE COUNT         1032 a         1011 c         1052 g          946 t
ORIGIN      
        1 cgtgtcatgc gggcgtcagg ctgccgtaat ggcaatttgc gcccggacca ggccgcaggg
       61 gtgaaactct gcggcctttt tcgtaagaaa aatgcaacga ttgcacgcgg ataatattct
      121 gcggaatgcg cagcacagag tgaagacgtg acggattccg gaacagaaaa acagtcgtag
      181 aatcagcggc aagccggaaa ggttccggtg aggcgcaatg ttgcgggggc tttatccctg
      241 gtggcattgg ttgctggaga gagaaaaccc ccgcacgttg caggtatgca cctgacaaca
      301 tcacgggggc taatcttgaa cccaaacaac tcaagaatag ccgcgaaacg ttggcattac
      361 aacacaggcg gctatatgac gctcgcagaa ctgggcatgg ccttctggca tgatttagcg
      421 gctccggtca ttgctggcat tcttgccagt atgatcgtga actggctgaa taagcggaag
      481 taacgtgtca tgcgggcgtc aggctgccgt aatggcaatt tgcgcccgga tcaggccgca
      541 gaggttaaac tctgcggcct ttttcgtgct tactgcgggt aaggcaccca gtcgccgccg
      601 ttcaggcgaa cgtacggttt atcctggtat tgaataacta ctgcatttga gttctcggag
      661 accggtgctg tttgtggcaa cccactggtg agttttttcc agtcaacatt gtcttcggtg
      721 aaaatcttgc catcaagaac gcgaaccacc agatcggaga tagctaggaa gctgctcggt
      781 tggtcaatga caatcggtgc cccctgatgt ggtgccttca tgccgaagaa tttcacccca
      841 acggggacgt ctgtgataga cgggctaggg atatcacgta ggccagatac ctgcattctg
      901 tcgcccttca gcgcgccgcc gtgttccggc accacgacca ccatcacttt acgacccgat
      961 ttctccagtt cagtaaagaa ggcgtccagt tcatcaaaga atttctgcgc ccgcgctttg
     1021 taatccgctg ttttgctgac ccccggataa tggttgccgt catgcagtgg aagcgtgttg
     1081 tagaacgtgg cactacggct gtttttatct ttttcggtaa cgtccagcca gcggttaagc
     1141 acggcggtat cgtcataaac cggcgaacca tcaaagccca gcaaaataac cggcagattt
     1201 gtttgatcca tcaattcagt ctgcatgccg ccattttcgc gaacttcttt caaaaaaccg
     1261 ccgaactggc cgttatgtcc catcatcagg tgctgggtaa agcccagttt cgaaaggtta
     1321 tcaaacagat agcagtcgtt atttgccggt tgatacagat tagtgtgcga agtctgcccg
     1381 cagctggcgc gcagtaaacg gatcgccgcc gggccactgt aggaggtggc ggagttaaag
     1441 ttcttgaact caatatcgaa atgcgaccac agtggatgcg acatcaaccc ggcggcttct
     1501 atatccgacc aggaaagcga acagatgtta atcaccagta gttcaaatgg ctgagcatca
     1561 gcgggcagcg aagacgggaa ggtcgattta cgtttcgcct ccgcgttata gaaattattc
     1621 agccaggcgt taaggttcgc cgttgttggc ggtgcagttt gtgcgggcat atcacccact
     1681 accgatgcgc cacccgtcgc cgcaacggtt gccgctgcgt taccacccgt cgttgttaca
     1741 gtggtcgtcg gttgtccggc tggccacaag gagaaacttg gtcccgccag ggtaagtacg
     1801 ttcagccata gcagtatggc aaccacaaaa acggtaatgc gaatccattg tgacaggaat
     1861 aaccaggcca ctaataaaac aaaaatggcc ccaatcatct gccagttaat aaagcgtgtg
     1921 acaaggtcga ttaaataatc ggtactgaac cccgccacct gcgaaccctg gctcattatg
     1981 ctttccgggc caggcaacca ggtgtcatgc cagaacaaag caaagccgat cggcagggca
     2041 atccagtggc gcaagcgatg caggctgtag cgcggaatgg gcatcagcag aaacgtggca
     2101 aacaccaaat tgaggagcgg atggaagtta agatatcccg cccacaacag gccgaactta
     2161 accagaaaat agaagttcca gccggaaagg ccgcgccagt attgccagag ggaagaaggc
     2221 atggcggtat tttgcgtaaa ttgagtcatt ttttggttgc cctggctttt tccgtgcggc
     2281 gtaacgtccc ggccggttta acataacgag gtttagcaaa gaataaacgt aaggtgtcgc
     2341 gaatgcgtcg caaagagtgc cgcgccagat agcccagcgg gaaaaatatc agtgcgcaaa
     2401 cgacaataat ttcaatgata tcgctgatgg tcatcatgat gagcgctcca cagcatcatc
     2461 taacagtcgc atgggttctg gtattcgtcg ccagtggcga ccatcgtgct cggcattgat
     2521 gaccggttta gaactttgcg ttaaaggcag cggcatgccc cattgttctg gggcaagcaa
     2581 gcgcatctgc accagctcgg cactgatttg atcatcttca aaccagacca tacggtttga
     2641 gaaaatgtcg ccagtaggca atgggaaaat atgattcaac gcggtatcca gatcgttaat
     2701 ccgacagaat gagagaaaca gcaccagccg attaccgcca atggtcatga tatcgccggt
     2761 acggttaggg cgacacagcg tcagggcttg ttcaacgcgg atacccggta ccggacgtag
     2821 ggcaaccaga acgcctttac cgtgggcagg taatagaggg ttattcatca tgttgttgac
     2881 ggcattacag aacacatccc acttctggaa accacgcagt ttgagcggct gggtcattga
     2941 cagcaaggta gtgatatctt ccggcacata gcgactaaac ttctgccctt gcacgctttc
     3001 gatcatcgtc agacaacggg agagtggcgc attccacgga ataaccatat ttgcaccgca
     3061 ggccaataac aaacgttcat cggtggcgcg caggctagcg gtattttccc gcacgaggat
     3121 tttcatcgca ctaccgcgct ggcgacgcag ggtatgaatg ctgcgggcca gtggctcgat
     3181 ttgcgcattt tgctgtaaag aaaagaccac cgtcgccgcc tgagcggtac gggcttcatt
     3241 gaacaggact tcgttattgt tgaacagttg ccagtgttcc gatagcggcg gcgcaccttc
     3301 cagtacagca acattactca gaatgcgttt ttcgtcgctg cgtggttgga tctccgcctc
     3361 ttcgctttga actaatgtcc agataccatt ttgttgctga acgctaagct gctgacgggc
     3421 gctgacccct ttttcgttgc accagaaggc aatatccagc aaatgttggt caccctgaaa
     3481 acgcaaactg gcaagaccaa aaagtgaacg gtattcctca agcaacaatg aaaattgttt
     3541 atcgttatta tttccgggat taattaccaa aagcgaacaa tggtttaacc tgctccattt
     3601 attcatttta tccaaccatg agcgaagccg ctcggcagga atgttttgcc atgcgttatt
     3661 tgcgcaaaca agaataaaaa ggtaattatg gggatcaata gaacactgca aatcacgggc
     3721 caaatagtat agaccttttt catgatttag cattgaaaat aattttattt tttccggccc
     3781 ttgagaatca tctaattgaa agattttcgc cggatcgctg tccatgctaa tgaccgcgac
     3841 gtgtgcggtt tcagcctggg atgcaattgt ttgattcgcc agactgatag catcttcatg
     3901 gcgatcgacg ttaaaccacc agacgccgcc tgctggcata tgtcgcagct catcccataa
     3961 tgatgagata ccgatagaga atacagggtc cacaatgtcc ctcatgtcac cattttgtct
     4021 gattatcagt ttactagcga a
//
LOCUS       DAZPDU010000273         3953 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZPDU010000273 DAZPDU010000000
VERSION     DAZPDU010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3953
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.277"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(50..649)
                     /locus_tag="ACHURS_004300"
     CDS             complement(50..649)
                     /locus_tag="ACHURS_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFR1239875.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYAGDKNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
     gene            complement(720..>3953)
                     /locus_tag="ACHURS_004301"
     CDS             complement(720..>3953)
                     /locus_tag="ACHURS_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFR1239876.1"
                     /translation="ISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITRTI
                     TSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTTSQ
                     YLASVMVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALVGV
                     QVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCLWD
                     MLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRKAW
                     DVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSALKD
                     RHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLWLI
                     KTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQIRTLTLDRE
                     ITLPSSGTTLISLVDGSGNPVSVEVQSVTDRVKVKVSRIPDGVAEYSVWGLKLPTLRQ
                     RLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHLTA
                     EVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRFRQLA
                     LGRYTLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDPTV
                     QFEFWFSETRIADIRQVETTARYLGTGLYWIAASINIKPGHDYYFYIRSVNTVGKSAF
                     VEAVGRASDDAEGYLDFFKGKITESHLGKELLEKVDLTEDNASRLDEFSKEWKDANDK
                     WNAMWGVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETPMF
                     VAQGNQIFMNDVFLKRLTAPTITSGGSPPVFSLTSDGKLTAKNADISGSVNANSGTLN
                     NVTINQNCTIKGMLEATQVRGDFVKAVSKAFPKKVGTWGNTETPDGTVTVTISDDHNF
                     DRQIIIPPIIFNGIAYDDPGSGNNPGGTRYTGYGFEVRKNGVLIASRETKGAIPGSYS
                     AVIDMPSGGGSVTLEFKIFQKGNQGAGNITDCTVIVTKKAASGISIR"
BASE COUNT          814 a         1216 c          998 g          925 t
ORIGIN      
        1 aagcccgcct gaaccggcgg gctgtcataa cactgtgtta cctggctaat cagaacttat
       61 aaccgacacc cacgatgaaa ccgtcagtgc gccagtcgcc actgccggag ccttcataag
      121 caatatcaat ggccacggat tcggtcgggt taaactgcac gccagctccc cacgccagag
      181 acgtgttgct gtggcgaccg tcatcacttc cggtcagcac atcgtgcgtt ttccccttgt
      241 tgtcagttac gcgaagataa tccccggaga aagtcgacac acggctgtaa gccacacccg
      301 ccatcgcata cgcgctgaac cattcattca cgcgcacaga cggccccgcc attacgctga
      361 accagcggtt acgaacggaa tcttcatgcc agcgggtatc gctgtaacgg gtcagctggc
      421 gattcttgtc tcctgcatag ctgaatgacg tcaccagccc caacgtgtcc gtaaactcat
      481 aacggtattt cacgttaatc ccgttcagat catcactgcc gggaacgttc gtcgaggcat
      541 gaagataccc cgcgctcagc gtggactgat gttcagacgc ccatgcaggc gcaccggata
      601 cggccagaca aatggctgcg gacaaaattg ctgcacaaac tttacgcata attacctctc
      661 gcttttctgc aataaaaaag gcgtcattcc tgacgccctt tattggggtt ataaatattt
      721 caacgaatac tgatgccgga agcagctttt ttggtcacaa tcaccgtaca gtcggtgata
      781 ttgcctgccc cctgattgcc tttctggaaa atcttaaact ccagagtgac gctaccacca
      841 ccactaggca tatcaataac tgcactgtaa ctaccgggaa tggccccttt agtttctctg
      901 gatgcgatta atacgccgtt tttgcgaact tcaaaaccat aacccgtgta tcgcgtgcct
      961 cctgggttat ttccgctccc cggatcgtca tacgctatac cgttaaaaat aatgggcgga
     1021 ataataatct ggcggtcaaa gttatgatca tcgctgatgg tgactgtaac cgtaccgtct
     1081 ggtgtttccg tgttacccca cgtaccgact tttttcggga aggcttttga tacagcttta
     1141 acgaaatccc ctctgacctg ggtcgcctcc agcatgccct taatcgtaca gttctggtta
     1201 atcgtgacat tgttgagcgt tcctgagttc gcattcacac tgccactgat atccgcattt
     1261 ttagcggtca gctttccgtc tgatgtcagg gaaaataccg gaggactgcc accgctggta
     1321 atggtggggg ccgtcaggcg cttcaggaac acgtcgttca tgaatatctg gttgccctgc
     1381 gccacaaaca tcggcgtttc attcccgttt gccgggtcaa taaacgcgat acggttagcg
     1441 gcaaccagaa actggctcag cttgccttcc tccgtgtcct ccatgctgag gccaataccc
     1501 gcgacataat gtttgccgtc tttggtctgc tcaattttga cgccccacat ggcattccat
     1561 ttatcgttag cgtccttcca ctctttcgaa aactcatcca gtctgctggc gttatcctcc
     1621 gtcagatcga ctttttccag cagctccttg ccgagatgag attcggttat cttgcctttg
     1681 aaaaaatcca ggtaaccttc cgcatcatcg ctcgcccgac cgacggcctc cacgaatgcc
     1741 gatttgccaa cggtgttcac actgcggata taaaagtaat aatcatggcc cggtttgata
     1801 ttgatactgg cggctatcca gtacagcccc gtgccaaggt agcgggctgt ggtttcaacc
     1861 tgcctgatat cggcaatccg cgtttccgag aaccagaact caaactgtac cgtcgggtca
     1921 taaacggcaa gatgcggcgt ggcggttatc tgaaaatagc ccggcgtcag ctcaatccgc
     1981 gacggcgctg ccggggcggc aatccggaac gataccgatg ccggatcgcc ctgctgtccc
     2041 cacgcattta ccgcccggac cgtcagcgtg taacgcccca gcgccagttg cctgaagcgg
     2101 tatgtggttt ccgtcgtccg ggccgtgctg accagccgct cactgccgtc atccgctgtt
     2161 acggtcagac ggagcaggaa gctcacgccc ttcaccacct tcggcgtgtc ccagcgcgcc
     2221 agcacctgat attccccgct gtctgcggtg acttcggcgg tcaggtgctg caccgctggc
     2281 ggcgtgacac cattcaccgt gccgctctgg tcgccgtcaa agtgcgcccc gttatccacg
     2341 atggcctctt tttccggtac atgctgcacg gcggtgatgg catacgtgcc gtcgtcgttc
     2401 tcacggatac tcacgcagcg gaacaggcgc tggcgcagcg tcggcagctt cagcccccac
     2461 acgctgtatt cagcaacgcc gtcaggaata cggctcactt tcaccttcac gcggtcggtg
     2521 acggactgaa cctccacgct gaccggatta ccacttccgt caaccaggct tatcagcgtg
     2581 gtaccggagg atggcagagt gatttcacgg tcgagcgtca gcgtccggat ctggctgttt
     2641 accgccagca cgcgcccgcc ggtgctgata ccggcatagt catcatcaca gatttcaatg
     2701 acatcgcccg gcacatggcg aagcccttct gcgcccacgc tgaagtccac ggtctgcgtt
     2761 tccagcagtt ctgttttaat cagccacagc ccggcgcggt gtgcctgccc ccggctagta
     2821 cagccaaaag catccatctt cgtgacgtta cgaccgtaac gggcaatggc ctgcgtatcc
     2881 tccacaagct ctgtcgccgt ctcccagccg ttatccgggt caatccagtt cacctcaacg
     2941 gcattatggc ggtctttcag ggcgctaaag ctgtagcgga acggcgcgcc atcatccggc
     3001 atcaccacat tactgcggtt ataggtccac accttatccg acggtcggtc ctgcacgaac
     3061 gtcagcgtct gcccgttcca taccggcata cagcgcatcg ccgagcagaa atcactgagc
     3121 acatcccacg ccttgcgctg tgtggtcagg tacgcattac aggtgatgcg cggctccgtg
     3181 ccgccaaagc cgtccggcac tgactggtcg cagtactggc cgatgacata cagcgcccat
     3241 ttgtccacat ccgccgcacc aagacgtttc cccatgccgt agcgcgggtg ggtcagcata
     3301 tcccacagac accaggccat gttgttgctg tatgctggtt ttaacgttcc gtcccagata
     3361 ccgctgtatt gtcgcgtctg cgggttatag ttcgacggca cctgcagaat gcgcccgcga
     3421 agatgataat tacgactcac ctgctggctg ccgaactgct ccgaatccac ctgtacgccg
     3481 accagtgccg tgttcgggta gcactgtttc acatcgatga tttcggtgta tgacgaccag
     3541 agcgttttgt tctgcagctg gtctgtagtg ctgtccggtg tcatcctgcg catccggata
     3601 ttgaacgggc gcggaggcag gttatccacc atcaccgagg ccagatactg cgaggtggtt
     3661 ttgcccttaa tggtgatgtc tttttccgtc acccagccac cgttacgctg tatctgaacc
     3721 agcaggcgga cttccgacgg attccggtca ccctttgagg tggtttccac cagtgcctgc
     3781 acgccgaaag taaaacgcag acggtcaatg tttgccgacg tgatggtccg ggtgatcggc
     3841 gtgtcatatt tcacttccgt acccagcacc gtctcggagc cggaggattc aaagccctcc
     3901 ggcggtgtct gctcctgctc acctgcccgg aacaccaccg tgacgccgga gat
//
LOCUS       DAZPDU010000274         3946 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZPDU010000274 DAZPDU010000000
VERSION     DAZPDU010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3946
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.278"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            30..968
                     /locus_tag="ACHURS_004302"
     CDS             30..968
                     /locus_tag="ACHURS_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFR1239877.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDNPPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            1024..2313
                     /locus_tag="ACHURS_004303"
                     /pseudo
     CDS             1024..2313
                     /locus_tag="ACHURS_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            2310..2603
                     /locus_tag="ACHURS_004304"
     CDS             2310..2603
                     /locus_tag="ACHURS_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFR1239878.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            2768..3946
                     /gene="fadK"
                     /locus_tag="ACHURS_004305"
                     /pseudo
     CDS             2768..3946
                     /gene="fadK"
                     /locus_tag="ACHURS_004305"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
BASE COUNT          929 a         1003 c         1088 g          926 t
ORIGIN      
        1 cctgaactga agcctacgga gaagaaacga tgagtcaatt aaacagcgtc tgggtcttta
       61 gcgataatcc tgaacgttat gctgaactgt ttggcggcgc gcagcaatgg ggccaacagg
      121 tgtatgccat tgtacaaaat accgaccagg cgcaggcagt tatgccttat ggtccaaaat
      181 gtatttatgt tcttgagcaa aatgatgcgc tgcaacgtac tgaaaattac gccgaaagca
      241 ttgctgccct gctgaaagat aacccccccg ctatgttgct gctggccgcg acgaaacgtg
      301 gtaaagcgct ggcagcacgg ttaagtgtgc aactgaatgc ggcgctggtg aacgatgcca
      361 cggcagtgga tattgtcgat ggtcacattt gcgccgaaca ccggatgtat ggcgggttag
      421 cgttcgctca ggaaaagatc aacagcccgc tggcgatcat tacccttgca cccggtgttc
      481 aggaaccgtg caccagtgat acctctcatc agtgcccgac agaaacggta ccttatgttg
      541 ccccgcgtca tgaaattctc tgtcgcgaac gccgtgcgaa agcggcaagc agcgtggacc
      601 tgagcaaagc aaaacgtgtg gttggcgtcg gtcgtggtct ggcagcgcag gatgacctga
      661 aaatggtcca cgaactggcg gcggtgctga atgctgaagt cggctgttca cgtccaattg
      721 ccgaaggcga gaactggatg gagcgtgaac gttatatcgg tgtctccggc gtgttgctga
      781 aatccgatct ctacctgacg ctggggatct ccgggcagat ccagcatatg gttggcggca
      841 acggcgcaaa agtgattgtc gccatcaata aagataaaaa tgcgccaatc ttcaattatg
      901 ccgactacgg tctggtgggc gatatctaca aagtcgtccc tgccctgatt agccagttga
      961 gccgctaatt cccctttcaa ccacatctcc cgctgtaaac gcagcgggaa gggagcataa
     1021 cgcatgtcgg atgacaaatt tgatgccatt gtggtcggtg cgggcgttgc tggtagcgtt
     1081 gccgcactgg tcatggcgcg agccgggctg gatgtcctgg tgatagaacg cggcgacagt
     1141 gccggatgta aaaacatgac cggcgggcgt ctttatgccc acacacttga agcaatcatt
     1201 ccaggctttg cagtatcagc gccggtagaa cgcaaggtca cacgcgagaa aatctccttc
     1261 ttaaccgaag aaagcgccgt taccctcgat tttcaccgcg agcagccaga tgttccgcaa
     1321 cacgcatctt ataccgtatt gcgtaatcgt ctggacccgt ggttgatgga acaagccgag
     1381 caggcgggcg cgcagtttat cccaggcgtt cgcgtcgatg cgctggttcg tgaaggaaac
     1441 aaggtaactg gcgtgcaggc tggggatgat attctcgaag cgaatgtggt gattctggct
     1501 gatggcgtta actcgatgct tggccgctcg ctgggaatgg ttcccgcttc cgatccgcat
     1561 cattacgctg ttggtgttaa agaggttatt ggcctcacac cagaacagat caacgatcgc
     1621 tttaatatta cgggcgagga aggtgccgcc tagctgtttg ccggttcccc ttctgacggc
     1681 ctgatgggtg gcggattcct ctataccaac aacgattccg tatcgttggg gctggtttgt
     1741 ggattgggtg atatcgccca tgcgcaaaaa agcgtgccgc aaatgctgga agattttaaa
     1801 caacaccccg ccattcgccc actaattagc ggcggcaaac tgcttgaata ttccgcgcat
     1861 atggtgccgg aaggcggtct ggcgatggtg ccgcaactgg ttaacgaggg cgtgatgatc
     1921 gttggtgacg ccgcaggctt ctgtctgaat ttgggtttta cggtccgcgg catggattta
     1981 gccattgcat cggctcaggc tgccgccaca acggtgatcg ccgccaaaga acgcgaggat
     2041 ttctccgcca gcagtctggc gcaatacaag cgtgagctgg aacaaagctg cgtcatgcgt
     2101 gatatgcagc attttcgcaa gatcccggcg ctgatggaaa acccgcgcct gtttagccaa
     2161 tacccacgaa tggtagccga catcatgaac gagatgttca ccatcgacgg caaaccaaac
     2221 cagccggtac gaaaaatgat catgggacat gcgaagaaaa ttgggctgat caacttgctg
     2281 aaagatggca ttaagggagc aaccgcgcta tgagccagaa cgctacggtt aacgtcgaca
     2341 tcaaattagg cgtcaataaa ttccatgttg atgagggcca cccgcatatc attctggcgg
     2401 caaatcccga catcaatgaa ttccgtaaat taatgaaagc ctgccctgcc ggactttata
     2461 agcaggatga cgccggcaac attcattttg attctgccgg ttgtctggag tgcggcacct
     2521 gtcgggtgct gtgcggtaac actattctcg aacagtggca atatcccgca ggcaccttcg
     2581 gtattgattt tcgctacggc taatcatgca tcccacaggc ccgcatctcg ggcctgatgt
     2641 tctgtttcga gagtccaaca tgaaagtgac attaacgttc aacgaacaac gtcgtgcggc
     2701 gtatcgtcag caagggctat ggggcgatgc ttcgctggcc gattactggc agcagaccgc
     2761 tcgtgcgatg ccagacaaaa ttgccgtggt cgataatcat ggtgcatcgt acacctatag
     2821 cgcgctcgat cacgccgcga gctgtctggc aaactggatg ttagcgaagg gtattgaatc
     2881 aggcgatcgc atcgcatttc aactgccagg ctggtgtgaa tttaccgtta tctatcttgc
     2941 ctgcctgaaa accggtgcgg tttccgtgcc gctgttgcct tcctggcggg aagcagaact
     3001 ggtgtgggtg ctcaataagt gtcaggcaaa aatattcttt gcaccgacgt tgtttaaaca
     3061 aacgcgtccg gtagatttaa tcctgccgct gcaaaatcag cttccacaac tacaacaaat
     3121 tgtcggcgtg gacaaactgg ctcccgccac ctcttccctc tcattaagtc agattatcgc
     3181 cgacaatatc ccactgacca cggcgataac gacccacggc gatgaattag ctgcggtgct
     3241 gtttacctcc ggaaccgagg gtctgccaaa gggcgtaatg ctaacgcata acaatattct
     3301 cgccagtgag cgggcttatt gcgcgcggct gaatctgacc tggcaggatg tctttatgat
     3361 gcctgcgcca cttggtcacg caacgggatt tctgcatggc gtaacagcac catttttaat
     3421 tggtgctcgc agcgtgttgt tagatatttt cactcctgat gcatgtctcg cgctgcttga
     3481 gcagcagcgt tgcacctgta tgctcggcgc aacgccgttt gtctatgatc ttttgaattt
     3541 actagagaaa caacccgcag acctttcagc gctgcgcttc tttctttgcg gcggtaccac
     3601 aatccccaaa aaagtggcgc gcgaatgcca gcagcgctgg cattaaatta ttaagtgttt
     3661 atggttccac agaaagttcg ccgcatgcgg tggtgaatct cgatgatcct ttgtcgcgct
     3721 ttatgcacac cgatggttac gctgccgcag gtgtagagat taaagtggtc gatgacgcac
     3781 gcaagacctt accgccaggt tgcgaaggtg aagaagcctc gcgtggcccc aatgtgttta
     3841 tggggtattt tgatgaacct gaattaaccg cccgtgcgct ggatgaagaa ggctggtatt
     3901 acagcggcga tctctgccgt atggatgagg ctggctatat aaaaat
//
LOCUS       DAZPDU010000275         3939 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZPDU010000275 DAZPDU010000000
VERSION     DAZPDU010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3939
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.279"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..588
                     /gene="fimZ"
                     /locus_tag="ACHURS_004306"
     CDS             <1..588
                     /gene="fimZ"
                     /locus_tag="ACHURS_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HFR1239879.1"
                     /translation="RMSIEVLLQKNSELQIVLKTDDYRITIDYLRTRPVDLIIMDIDL
                     PGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANGFVSKCNDQNDIFHPV
                     QMILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRYLVSGLSNKEIADKLL
                     LSNKTVSAHKSNIYGKLGLHSIVELIDYAKLYELI"
     gene            complement(591..1106)
                     /gene="sfmF"
                     /locus_tag="ACHURS_004307"
     CDS             complement(591..1106)
                     /gene="sfmF"
                     /locus_tag="ACHURS_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HFR1239880.1"
                     /translation="MRRVLFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVQPGVE
                     KADAIFMINYN"
     gene            complement(1117..2124)
                     /gene="fimH"
                     /locus_tag="ACHURS_004308"
     CDS             complement(1117..2124)
                     /gene="fimH"
                     /locus_tag="ACHURS_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFR1239881.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNVFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAIKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            complement(2137..3911)
                     /gene="fimD"
                     /locus_tag="ACHURS_004309"
                     /pseudo
     CDS             complement(2137..3911)
                     /gene="fimD"
                     /locus_tag="ACHURS_004309"
                     /inference="COORDINATES: protein motif:HMM:NF012786.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein FimD"
BASE COUNT         1097 a          971 c          861 g         1010 t
ORIGIN      
        1 agaatgtcta ttgaagttct gttgcaaaaa aacagtgaat tgcagattgt cctgaaaacg
       61 gatgattacc gcataaccat cgattatctc cgaacccgtc ctgttgattt aatcattatg
      121 gatatagact tgcccggaac agacggtttt accttcctga aaaggatcaa acaaatccag
      181 agcacagtga aagtgttatt tttatcatcg aaatcagaat gcttttatgc tggcagagcg
      241 atacaagctg gtgctaacgg ttttgtcagt aaatgcaatg atcagaatga tatttttcat
      301 cccgttcaga tgatcctctc cggatacacg ttttttccta gcgaaacgct caactatata
      361 aaaagcaata aatgtagtac gaatagttca acggtcactg tgctatctaa tcgtgaagtg
      421 accatattac gttatctggt tagcggatta tctaataaag aaattgccga taagttatta
      481 cttagcaata aaacagttag tgcgcataaa tctaatattt atggcaaact aggtttgcat
      541 tcaattgtag agcttatcga ctacgccaaa ttatacgaat taatataata ttaattataa
      601 ttgatcataa atatcgcatc cgctttctcc acacctggct gaacaccgct ggctaacgct
      661 cgataattgg caaaaaagtt tagtgtgaca ttgccatttg catctacttc ctcagtcggg
      721 ctcgcctccc ccagtgcgag ccgggagcga tcgctattac gtaattcgat ggcgacggtt
      781 tgtgccattg cgggatcatc cagagccagc aggttggtat cggatgccgg cgttcccgta
      841 aataaaatcg caactgaacc cggaggacat ccctccagcc gcaggctaaa agggacgagt
      901 gccgtggtat cgccagcgtt cagtaattgt gtcgtaggcc atctgcctaa atctaccgtc
      961 ttatcaatat ccgcagtgtt tacggtacag gagaaatcaa caacgttacc gtgcaaattg
     1021 atattaatcg ttcccaaagg gtcagctgcc catccactgg aactccacag taacccgcag
     1081 aaacagctaa agagtactct tctcattctc cgtacctcat tgataatcga cgcgtaaata
     1141 ccccagcgcg ctaaacggcc cttcggtcgg tttttgaccg gtaatactga taggccaggc
     1201 gcgaagtgtg acattggctg ccgcagctgc atccagacgg aaaggaataa cgctattgag
     1261 atcgttaggc gtgatcggcg tatcgttctg atcggcgaca ataaaaccta aatcctgatt
     1321 gtccgacacc atcgcctgac cagaaacggc actggcttcc agacgcattg ttaaataagc
     1381 ctgcgcagca acattcgtac atttgatcgc aatgctcttg gtttgcggca tgacgccagc
     1441 tggtcgattc cccggccctg ccgcactaaa taacgatgcg ccgatatcac caaaatcaaa
     1501 ttcaacaatc tgcccggcat ttaattcgca gttttgcggc acttcaaccc ggccaccaaa
     1561 actaatggta taaacaggtg taaccaacgg atcgtaaggc gttgatgtca catacacggt
     1621 aaacatcacc tgtctgggga tctccaccat gttaataaag ggacgaataa cctttaaacg
     1681 aaatatcaaa cgagaatcca tcaccccgaa cggaaggttt tgcgaaacgt taggatctgt
     1741 tcccatccga atataatttt gcggggggaa ctgaatatcc atcacactat caaccagact
     1801 catcgcacct aatagataat catttaattg catatattta taattatcga tagtttcctg
     1861 aacaataaag ttagtgacat aactacggta tgtataattc accagcgtgc cgggtggaca
     1921 aattgctgag acacctaccc agccggattt ttccggtaaa acaacaatct gccctggctg
     1981 gttattggtg ctattaaaga catttgagag atcatagtgt tcatcttctg ctacgccagt
     2041 actatttgca caaacagtag cccaggatat gtttgccaga cacaaacatg ccattgccaa
     2101 taataatctg cagattatct tcattgataa tttcctttat gagccaggat gtgtgcaaac
     2161 tgccgaaata acagtgacgg cttgctgtaa gctttgcttt ggcaatacat agtgcgcaat
     2221 gcagcgtgag ttcgcgccat ctccccactg aaccagtaat tcaccagaca atggcgcacc
     2281 acttaaataa acttgcccgt catcacccac catactggtt attccggtac tgttttcccg
     2341 taccagtgat ccaaacggca ccggttttcc gccctgggta acggtaatga gcgcccgcac
     2401 gcctatgcgg gtatcaaaat tggcacgaac caacgcgcct tgcgtcggca caacgctgct
     2461 aatatttttt tcaacatcga tggaattccc catcgtattg gtatcaagcg cgatacggtt
     2521 ataccgataa accgtggcat acggcatcac cgcatagccg cgccagtcgg ttaaaatgcc
     2581 agtttgattt tcaatgcgta caccgcctgc gccaggcgct ttaatcaaaa cattggtatc
     2641 ccctaaaggc tggctcagtg ttatgccatt ttcatgcccg accacaccgc cagacagctg
     2701 ccagttaacg tcatgttgat cgcggtcgta gttatacccg acccccagcg taccgtaagt
     2761 ggcctgccag tttgccgtgg cgctgcccgt gtacccatta ttcgaggtat cgccctggct
     2821 tacgtgataa ctcaggttgt ggccttccag taaggtccca ccaacgcctg ccagccagct
     2881 attttgcccg ttgctgttac ggttagcgtt caaggaggca taagcgcggt cgagcgcatt
     2941 ttcccgggtg tagcgacgtt tggtcaaaac attgaaaggc actgaaacat taagtccgac
     3001 aatatgttcg ttatcgggga tccctacaga ttcattccac gaaaatgaga gtgaataact
     3061 gatgccaacc cagctgctgg tataccccac ctgataccag gtatctgaat ccgaagtatt
     3121 ccagtatttt tgatgggtac cagagatata aagcgagcca aagtcattaa gtgattgtga
     3181 aatatttaac tgcaaacggt ctttgcggct aaagcgtaaa ttgtggtaat tcacgattat
     3241 cggttcatcg cgatgctcac cgtcgtaatc gtaatcatat tcgtacccct ccattcgacg
     3301 atacgcaaca tcatcaaggg tataaaaacc ttgtgtcgaa tagcggtaac ccattaactg
     3361 aaaattggtg ccgaaggtgt acatcgattt cgcatagagg aagcgaatgg aatccccctc
     3421 atgacgactg tcgtcggcta actggctgcg cgcatgggtc acatccagcg acactgcgcc
     3481 ccagttcccg aggttgcgcc ccagccctaa taaaaaggcg gtgtaattgg cagataattg
     3541 cgtcccgccg taggcagtaa attcctgtgg taaaccaccg agtgccgtgc cctgaaaaaa
     3601 gaaaggtgat gattgctgac tattaccgct acgaaaatcg cccgccgtca ggtcaaattt
     3661 gaaacgccct tcgcgttgta aaatcggcac tgttgaatac ggaattgtgt aattctgcca
     3721 tcgcgctcgt cgatggtgac atccagatcg ccatttgaag atgtcgggtg caaatcagta
     3781 atttcaaaag caccggggga aacatagctt tgatagataa taaaaccatt ttgccgaatc
     3841 gttagctggg ccgccgtacg ggcaatccca cgtaccgttg gggcaaaccc ttgctggcta
     3901 tctggataca tattatcgga agaataaagc cgtacacca
//
LOCUS       DAZPDU010000276         3911 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZPDU010000276 DAZPDU010000000
VERSION     DAZPDU010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3911
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.280"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..255)
                     /locus_tag="ACHURS_004310"
     CDS             complement(<1..255)
                     /locus_tag="ACHURS_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR1239882.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCMELVASGDVYNSGYGVFPSEQAHKDWQNARKKLSRVKV"
     gene            complement(270..1016)
                     /locus_tag="ACHURS_004311"
     CDS             complement(270..1016)
                     /locus_tag="ACHURS_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR1239883.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLADAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTLLGERV
                     MDRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            complement(1023..1331)
                     /locus_tag="ACHURS_004312"
                     /pseudo
     CDS             complement(1023..1331)
                     /locus_tag="ACHURS_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1376 domain-containing protein"
     gene            complement(1710..2222)
                     /locus_tag="ACHURS_004313"
                     /pseudo
     CDS             complement(1710..2222)
                     /locus_tag="ACHURS_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdaU family protein"
     gene            complement(2235..2657)
                     /locus_tag="ACHURS_004314"
     CDS             complement(2235..2657)
                     /locus_tag="ACHURS_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFR1239884.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELPLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSSDDSVIVH"
     gene            complement(2654..2896)
                     /locus_tag="ACHURS_004315"
     CDS             complement(2654..2896)
                     /locus_tag="ACHURS_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1239885.1"
                     /translation="MNKISTYRKQLGLSQRQLAVQLGWIQSRLANYEANFRTPGLEEC
                     RKIVSTLNRLGAHCGLDDVFPPDGKHSENSIGAVDS"
     gene            2993..3412
                     /locus_tag="ACHURS_004316"
     CDS             2993..3412
                     /locus_tag="ACHURS_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1239886.1"
                     /translation="MEKENEKIAASRLNDKIAMRLKERRQKLGLSQGKLAEICGWTQS
                     RIGNYEAGSRNVGVHDAVVLGKALGISPPELLFGEQESSELWLNESQRKLLELFNQLP
                     GSEQQRMIELFEVRLKEIDEYVEKYLRGRIKDNPPPE"
     gene            3718..3873
                     /locus_tag="ACHURS_004317"
     CDS             3718..3873
                     /locus_tag="ACHURS_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFR1239887.1"
                     /translation="MNTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LGRHSGE"
BASE COUNT          984 a         1049 c          868 g         1010 t
ORIGIN      
        1 cacctttacc ctcgatagct ttttgcgggc gttttgccag tctttatgag cctgttcaga
       61 gggaaatacc ccgtagccag aattatatac atcaccactt gcgaccagtt ccatgcataa
      121 gcgaccgaca gacgcacgac tgatacctgt ttcatctgac aactgccgaa tcgtgacccg
      181 accgtcctga tgcacgagtt ccacaattcg cgtcttcagt tcttcccgct gttcgggagt
      241 aaaaggtttc gccataaatc ctcctgaaac tacttaacaa ccctcgaatg gctaacattc
      301 ggacgccagc tctcccagtt aaaattcacc cagcgcccgc cgttcatgac catgcgatcc
      361 atcacccgct cgccgagaag tgtattcatc gctgcatggt taatatttgt cagcatcccc
      421 acaccgcgta acgatgccgt ccggcgatca acaatctggt tcagtaccac ctgctcgttt
      481 ttcgtatccc gctgcatgcc aatttcatcc aggaccagca ggtcaacacc acaaagctcc
      541 tgtaaaaatt tttcaccgga tttgccgttg tcgtagctct catgcaacac gctcatgaca
      601 tcggacacgg tgacgataat cacgctacgc cccttcgcca tcagccggtt gccaatcgcg
      661 tctgcaagat gattttttcc ggtgccgggc ttaccgctga acacaaaatt cgtacagcct
      721 gtcatcagtt cgtcagctat ggatttcgcc tgactcagtg catgtcgctg accgtcgttc
      781 tgcgcccggt aattcgcaaa cgaacacttc cggtgcaacg gctggatgcc ggagcggttc
      841 aggatttttt ccacccgcaa ctgacgattc aggcggttga tctcctcgca acgtttctgg
      901 ccttcagcaa gttgccactc gcgccactcc gcaaccgttc tgaatggggc ggttacatgt
      961 ggtggggtca gtctgcggat acgctccaga acgcctcctg tcgcaatatt tttcatggtc
     1021 tgttaccccc tgaagcctgg cgggatcgca ctgtccggca acgagacggt gttaacctgt
     1081 cggagcaacg tctcaggccg aacacctttc ggcgcgaaca gaccctggta ttcattggcg
     1141 atgctgtgtc gaatcacctg ctcaggtgta aaaccctgct gacggaattt ttccagttcc
     1201 cgtatcgccc cgttagcgcc ctgctccgtt cgaatcggtt ttcgcaatgc ctgcctgaac
     1261 tggacccact catgccagag tgtttctggc aaccagtcgg gcagatcaac tgacaacggg
     1321 tcaaattttt tctggcgaac agccccaccg ataaccagaa ttccctgctc ggtgtgcctt
     1381 gcgatatcac tgcgccgcaa gacacatatc ccggtttcaa cagcaccgat tttgagcagt
     1441 gatttctgtc gcggagaatt tcggtcaagc tcgctcccac accggacacc gttcctgtca
     1501 gtcacgacga tatcaatccg ccctccgcaa ccgtcaccac gctctggcac cggatactca
     1561 cgacagacat ccaggccagc agcctgcaac gcaaccaccg cagaattaca aaattccgct
     1621 gcagtaccgc cagataatct cccctcaagc actccagcca caaaatcccg aaactccctt
     1681 ccggaaggga agggtttggg atgggttaga tctgttttta gatctttatc tgtatcttta
     1741 ttagttgcct ttgtgttggc atcatgttcg aacatcactc caacatctgt ttgaacacct
     1801 gttaaatttc tctcttgttt tgtttgaaca tctgcttcct ttctgcttct tctggcctga
     1861 acagatgctt ttcctgcggc tgattttttg gttaattttt ctctgacaga tgccagatct
     1921 tcctcaatcc gaagatgcat ccattcgtcg ccgttatcac aaaaaaactc ccgcaaggat
     1981 ggttcaacat caacccatcg ctcgttagtc agacgggcaa tttttgccaa cctgtttttg
     2041 ggtattggct ttcctgtttg ccagtaattg aacatcagca acaaatacgc gccgtgctcc
     2101 tctgctgaca aatgcatggt gtcagccagg taatcagcta tgtacagttg catgtatggt
     2161 aatgcggcca taattgcccc gtatgatgct gcccggttgc ttagaataag cacaaacagc
     2221 atggaaactt ttgcttaatg aacaatgaca gaatcgtcgg aagacccgcc gccgctgaaa
     2281 tgcgctttcc ggtaaacggc ctggactgca tcatcatgcg catcaattgc cgtactcaac
     2341 gcttcctgcg ccgccagtaa tgcacggcgt tccagggtat cgaagatgca gagtcggtga
     2401 cgcagctcgc gcggaagaat tgccagaacc gcagggatca gtttctgaat tttttccctt
     2461 tgcgctttcg tttcaccttt caaccaacgg tgatagatat tctgctgatt gttccagtcc
     2521 ttgcctggta caaggggcaa ttcgccgccc ccctggcgca gatattcttc agtaattgcg
     2581 ttagcgaccc acgcctgccc tttttcggct gccagggcta acaacactga ttcgatgtgc
     2641 tcatgcctga ttttcatgaa tcaaccgctc ctatgctgtt ttcgctatgc ttaccgtctg
     2701 gggggaatac atcgtcaagt ccacaatgag cgccaagccg attaagggta gaaacaattt
     2761 ttctgcactc ctctagtcca ggggtacgaa aatttgcttc gtaatttgcc agtcggcttt
     2821 gtatccaccc taactgaaca gcaagttgtc tttgagacag cccaagctgt tttcgatatg
     2881 ttgaaatttt gttcattgaa aacctccgat gacaatttta aacacacctt gtgttatata
     2941 gccaagctgt tttgtgtttt atgtaaatca cgattcgtga tacaaggatg caatggaaaa
     3001 agaaaacgaa aaaattgccg ctagtaggct caatgacaaa attgcaatgc gtcttaaaga
     3061 gcgcaggcag aagcttggtt tatctcaagg aaaacttgct gaaatctgcg gatggacgca
     3121 atcgcgtata ggtaactatg aggcgggcag cagaaatgtt ggagtacatg acgctgtcgt
     3181 attgggaaag gcacttggca tatctcctcc tgagctcctc tttggagaac aggaatcttc
     3241 tgaattgtgg ttaaatgaat cccaacgaaa acttcttgag ttgtttaacc agctaccggg
     3301 ctcagaacaa caacgaatga ttgagctatt tgaagtccgg ctaaaagaaa tcgatgagta
     3361 tgtagaaaaa tatttgagag gcaggattaa agataatccc ccaccggaat aatgatcttg
     3421 ctatcacagt aatatgccaa tcagcccgct atcagcgggc ttttttgtag catcatcata
     3481 tgacactcac cataaaacac attgcgtgtt gacacaagaa aacaaattgt gtttaataag
     3541 catatccaaa caacgcccca ccagagaacg gcaggacaat acctcgagtt atccagtcac
     3601 tgaacagggc taagtagcca gcctgaggca tacgaacatg acggcagttg ttgattgata
     3661 caaagcgcag tagataaaac gttccgccac ccggcgttaa ggggaaatgg ggtcaacatg
     3721 aatactatcg atcttggcaa caacgaatct ctggtatgtg gcgtgtttcc caatcaagac
     3781 ggcacattca ccgccatgac gtataccaaa agcaaaacgt ttaaaaccga agctggcgca
     3841 cgtcgttggt tgggaagaca ttcaggtgag taaaatgaac gagacagaat taaaacacgt
     3901 tatcgctcta c
//
LOCUS       DAZPDU010000277         3908 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZPDU010000277 DAZPDU010000000
VERSION     DAZPDU010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.281"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            24..590
                     /locus_tag="ACHURS_004318"
     CDS             24..590
                     /locus_tag="ACHURS_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFR1239888.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            594..1169
                     /locus_tag="ACHURS_004319"
     CDS             594..1169
                     /locus_tag="ACHURS_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFR1239889.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1211..2263)
                     /locus_tag="ACHURS_004320"
     CDS             complement(1211..2263)
                     /locus_tag="ACHURS_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFR1239890.1"
                     /translation="MPVLHNRISNDALKAKMLVESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2488..3408
                     /gene="lpxL"
                     /locus_tag="ACHURS_004321"
     CDS             2488..3408
                     /gene="lpxL"
                     /locus_tag="ACHURS_004321"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFR1239891.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3580..3765
                     /locus_tag="ACHURS_004322"
                     /pseudo
     CDS             3580..3765
                     /locus_tag="ACHURS_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
BASE COUNT          971 a          987 c          952 g          998 t
ORIGIN      
        1 gcaaattcgt attggatgca cttatgtcat tcacaaatac ccctgaacgc tatggtgtta
       61 tatctgcggc cttccattgg ctaagcgcaa ttattgtcta cggcatgttt gctttaggtc
      121 tgtggatggt cacgctcagt tattacgatg gctggtatca caaagcaccc gaactgcata
      181 aaagcatcgg tattttgtta atgatggggc tggttattcg cgttctgtgg cgtgtcatat
      241 ctccaccgcc gggtccgctg ccgagttatt cgcccatgac tcgtcttgcc gcaaaggctg
      301 gacatctcgc attgtaccta ttgctgttcg ccatcggcat cagcggctat ctgatctcaa
      361 ctgccgatgg taaaccgatc agcgtttttg gctggtttga cgtccccgcg acccttgccg
      421 acgctggcgc acaggcagac tttgccggtg ccctgcattt ttggctcgcg tggagcgtcg
      481 tagtactgtc cgttatgcac ggatttatgg ccctgaagca tcatttcatc gataaagacg
      541 acactctgaa gcgcatgctg ggaaagtcgt catctgacta tggagtataa aaaatgaaaa
      601 aaagcctgct tggtttaacc ttcgcgtccc tgatgttctc tgccggttca gcggttgccg
      661 ccgattacaa aattgacaaa gaaggtcagc acgcctttgt taatttccgc atccagcacc
      721 ttggctatag ctggttatac ggcaccttta aagatttcga cggtactttt acctttgacg
      781 aaaaaaatcc ggctgccgat aaagtgaatg tgacaattaa caccaccagc gtcgatacta
      841 atcacgccga acgcgataaa catcttcgca gtgcagattt cctcaatacc gcaaaatatc
      901 cacaggcaac attcacctcc accagcgtga agaaagacgg tgacgaactg gatattaccg
      961 gcgatctgac gctgaatggc gtaaccaaac ccgtcacgct ggaagcgaaa ttaattggtc
     1021 agggcgacga cccatggggt ggtaaacgtg caggcttcga ggccgaaggc aaaattaagc
     1081 tcaaggactt caatatcaag acagatttag gtccagcttc tcaggaagtg gatctgatta
     1141 tttcagtgga aggcgtacag cagaagtaag cgtgaaatgt tgccggatgg cacgcatccg
     1201 gcaatgatat ttattctgtt ggatcaggaa tgcccagtgt tgtattcaga cgtccacgag
     1261 acttattaaa gatcttattg ccattttcac gtcctgcccg acggcgtcgc tgttcttctg
     1321 gcggtaacgc gctttcttcg cagcaaatct cactacaaca acctttgtat ttttccgcgc
     1381 atactggaca ctgaataaac agcaggtggc agccatcatt tttacagttg gtatggctgt
     1441 cgcacggcgc gccgcactgg tggcaatgcg cgataatctc atccgatata cgttcgccca
     1501 tccgctcgtc aaaaacaaaa tttttgccaa taaaacgcac cggcaagccc tgctcgcgcg
     1561 ccttacgggc gtattcaata attccgccct cgatatgcca gactttgttg aatccgttat
     1621 gtttcatcca ggcactggcc ttttcacaac gaatgccgcc ggtgcagtac atgacgattt
     1681 ttttatcttt atgtgcctgc atcatctcga ctgcttttgg cagctgctca cggaaggtat
     1741 ctgccggaat ttcgagcgcg ttttcaaagt gccccacttc atactcatag tggttacgca
     1801 tgtcgataaa tagtgcatcg ggatcgtcaa gcatggcgtt cacttccgcc gcttgcagat
     1861 actcaccaac attgctggca tcaaagtgag gatcgtcaat accgtcggca acaatgcgat
     1921 cgcgtacctt catgcgcagt acccagaagg atttcccgtc atcatccaac gcgatattca
     1981 ggcgtaaacc ctctaaagcc gggtcgaagg catagagctg cgcgcgaaat gtttcaacat
     2041 tgctcgcagg tacgctgatt tgcgcgttaa tgccctcatg cgccagatac actcgcccaa
     2101 aaacattcag cgcggtaaac agctgatata aagcgtcacg ggtcgcctta ggatcggcga
     2161 tgtggaaata cttgtaaaac gaaatggtgg ttcgcggttc gctctcaacc aacattttgg
     2221 cttttaacgc gtcgttggaa atgcggttgt gtaacactgg catggtgtac ggttcctgcg
     2281 agatgggaaa gtaaaaatcc gcggcatgat atagcaatta tcgataatta acatccacac
     2341 attttacgct acatttgcgc attaaaaatt atttgttatt tacaagcgcg gcaatttcgc
     2401 ccagtcttca gccacaaatt ttggttgcgg gcgaaaaaat gcgacaatac atacaattgc
     2461 ccgaataggt tgaaaaacag gattgatatg acgaatctac ccaagttctc caccgcactg
     2521 cttcatccgc gttattggtt aacctggttg ggtattggcg tactttggtt agtcgtgcaa
     2581 ttgccctacc cggttatcta ccgcctcggt tgtggattag gaaaactggc gttacgtttt
     2641 atgaaacgac gcgcaaaaat tgtgcatcgc aacctggaac tgtgcttccc ggaaatgagc
     2701 gaacaagaac gccgtaaaat ggtggtgaag aatttcgaat ccgttggcat gggactgatg
     2761 gaaaccggta tggcgtggtt ctggccggac cgccgaatcg cccgctggac ggaagtgatc
     2821 ggcatggaac acattcgtga cgtgcaggcg caaaaacgcg gcatcctgtt agttggcatc
     2881 cattttctga cactggaact gggcgcgcgg cagtttggta tgcaggaacc ggggattggc
     2941 gtttaccgcc cgaacgataa tccactgatt gactggctac aaacctgggg ccgtttgcgc
     3001 tcaaataaat cgatgctcga ccgcaaagat ttaaaaggca tgattaaagc cctgaaaaaa
     3061 ggcgaagtgg tctggtacgc accggatcat gattacggcc cgcgctcaag cgttttcgtc
     3121 ccgttatttg ccgttgagca ggctgcgacc acgaccggaa cctggatgct ggcacggatg
     3181 tccggcgcat gtctggtgcc cttcgttcca cgccgtaagc cagatggcaa agggtatcaa
     3241 ttgattatgc tgccgccaga gtgttctccg ccactggatg atgccgaaac taccgccgcg
     3301 tggatgaaca aagtggtcga aaaatgcatc atgatggcac cagagcagta tatgtggtta
     3361 caccgtcgct ttaaaacacg cccggaaggc gttccttcac gctattaaat ctcccctgcc
     3421 ggatgcttca gaatggcatc cggcattacc acagcaaatc cccctgattt agcgataaaa
     3481 gctctctgga ttgcgccccc tggaagtcgg gcgcataatt agtgtgctta tcttttcttc
     3541 ttatgttcac cgcgcctggc gcaccaacag cggattgcta tgtcaccctg tggaaatgac
     3601 acccctataa actggaaacg aaacctgatc gtcgcctggc taggctgttt tcttaccggt
     3661 gccgccttca gtctggtaat gcccttctta cccctctacg ttgagcagct tggcgttacc
     3721 ggtcactccg ccctgaatat gtggtccggt attgtcttca gtataatacc ttcctaaata
     3781 cactacctga gagaagagat gaaaagagct atcagctaca tgaggttttc aactgagaag
     3841 cagataaaag gggactctat acgaagacag tctaagctgg tcactgactg gttatcaaag
     3901 aaccctga
//
LOCUS       DAZPDU010000278         3857 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZPDU010000278 DAZPDU010000000
VERSION     DAZPDU010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.282"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..305)
                     /gene="chbB"
                     /locus_tag="ACHURS_004323"
     CDS             complement(<1..305)
                     /gene="chbB"
                     /locus_tag="ACHURS_004323"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFR1239892.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIK"
     gene            complement(604..942)
                     /gene="osmE"
                     /locus_tag="ACHURS_004324"
     CDS             complement(604..942)
                     /gene="osmE"
                     /locus_tag="ACHURS_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFR1239893.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            1144..1971
                     /gene="nadE"
                     /locus_tag="ACHURS_004325"
     CDS             1144..1971
                     /gene="nadE"
                     /locus_tag="ACHURS_004325"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFR1239894.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRLETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREADIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            2201..3088
                     /gene="cho"
                     /locus_tag="ACHURS_004326"
     CDS             2201..3088
                     /gene="cho"
                     /locus_tag="ACHURS_004326"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFR1239895.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(3048..3623)
                     /gene="ves"
                     /locus_tag="ACHURS_004327"
     CDS             complement(3048..3623)
                     /gene="ves"
                     /locus_tag="ACHURS_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFR1239896.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFAADQVVKAKLTEGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
BASE COUNT          985 a          957 c          919 g          996 t
ORIGIN      
        1 tttttaatcg ctgcaaccgc agccttaagc acgcctaaac catcgacttt gccataaagc
       61 agcgagtcaa ttacttcaac cggtttgttg ggtaacaaac gctggatttc gggcaacata
      121 taagcaatct gcggccctaa taacacgaca tcggcattct gacctttttc accagccagt
      181 gtttccggaa atgcttcaat aatgaccgga acttcatatt tttctgcctg tgcgcgcatt
      241 tttgatacca gtaaagaggt agacatgccc gcagaacaaa acagataaat gtgtttcttt
      301 tccataaaac tgccctcatc gacgattatc tgtcagccag acactccgca agccttaacc
      361 tgcttccatg ctctgggtaa cttgcgaaac caacatgact ttttttgtgg ctgaaaggag
      421 tatacggtat agacctgagc aatagtattt ctttgacctt ataaattgtt tctccttgac
      481 ctgtggttat gaccctcttc acatttcggg caaataattc gcgaagataa ttaagaactc
      541 aggccaaaaa aaaccggcgc aatggccggt ttccgttgtt actcaagctt tcagacgaat
      601 tgattacttc gcagcctgtg gatcagtgtc gtattcagca caggtctgat aaccggagtt
      661 gatgacatgt ccggtatcat ctaacgcgac aaagtaggtt tctgctttac catcacgttg
      721 acccaggatg taggtctggc aagtaccacg agcatggatc atgctcactt cagacgaagg
      781 tttacccgca atttgtgcaa cctgcgcccg gctcatgcct tttttgacgt ctttcaccac
      841 aggctgtaca aactggtctt tagtacgatc ataagccgta caacccgcca gcatggttaa
      901 taccgccgct gcactcagaa ttcctgccat attcttgttc atattccgtc ctcttgttta
      961 tcagcgtgtt agataagcct ggaatacatt gggcgctttt tcaagcccgc gaacgaaacg
     1021 gctccgcttt cagaggattc ctgtatgacg ttttaaccac cattcagccc gctgtcgctt
     1081 gtcgtttcag tggcaacggg ttagctttaa ggaagttttg tcttttctgt ctggaggggt
     1141 tcaatgacat tgcaacaaca aataataaag gcgctgggcg caaaaccgca gattaatgct
     1201 gaagaggaaa ttcgtcgtag tatcgatttt ctgaaaagct acctgcaaac ttatccgttc
     1261 attaaatcac tggtgctcgg gatcagcggc ggtcaggact ccacgcttgc cggaaagctg
     1321 tgccagatgg cgattaatga gctgcgcctg gaaaccggca acgaatcact gcaatttatt
     1381 gccgtacgcc tgccctatgg tgttcaggcc gacgaacaag attgccagga tgccattgcc
     1441 tttattcaac cggatcgcgt attaaccgtt aatatcaagg gcgcggtatt ggccagcgaa
     1501 caggcattgc gggaagcaga cattgaactg agcgattttg tccgtggcaa tgaaaaagcg
     1561 cgtgagcgga tgaaagcaca atatagcatt gcgggtatga ccagcggtgt cgtggtgggc
     1621 accgatcatg cagcagaagc cattaccgga ttcttcacta aatatggtga cggcggtaca
     1681 gacattaatc cgctgtatcg tctcaacaaa cgtcagggta aacagttact ggcggcatta
     1741 ggttgcccgg aacaccttta taagaaagcg ccaacggccg atctggaaga tgatcgccct
     1801 tctctgccag atgaagtggc actcggcgtg acctatgaca atatcgacga ctatctggaa
     1861 gggaaaaacg tacctgaaca ggtcgccaga acaatagaga actggtatct gaaaaccgaa
     1921 cataaacgcc gtccgccaat taccgttttc gatgatttct ggaaaaagta ataatttgca
     1981 cataatggtt tgtgcgaaaa agagtaattt gttcacgccg gatgcggcgt gaacgcctta
     2041 ttcgacctac aaaactatgc aaattcaata tattgcagga gtcgaatagg cctgacaggc
     2101 gtagcacgtc agacggtgta acctttgtca tcgacccgcc tcttttttaa tcgctccccg
     2161 cctgttacac tggatagata accagcattc ggagtcaaca gtggtacggc gtttaacttc
     2221 tccgcggctc gaatttgaag ctgcggcaat ttatgaatat cccgaacatt tacgttcatt
     2281 ccttaatgac ttacccaccc gaccaggggt gtatctgttt catggcgaaa gtgacaccat
     2341 gccgctctat atcggcaaaa gcgttaatat ccgcagtcgg gtgctttctc atttacgcac
     2401 cccggatgaa gccgccatgc tacggcaatc ccggcgaatt agctggatct gtaccgcagg
     2461 cgaaatcggc gctctgctcc ttgaagcgcg attaatcaaa gaacaacagc cgctgtttaa
     2521 taaacggttg cgccgcaatc gccagctctg tgccctgcaa ttaaatgaaa agcgcgtcga
     2581 tgtggtgtat gccaaagagg tggatttttc acgagccccc aacctgtttg gcctgtttgc
     2641 caataggcgc gcagctttgc aagcattgca gaccatcgct gatgaacaaa aactttgtta
     2701 tggcctgctg ggactggaac cgttaagtcg cggtcgtgca tgttttcgtt cagcgctaaa
     2761 acgttgcgcc ggagcatgct gcggtaaaga gagccatgag gaacatgcgc tacgcttgcg
     2821 ccagtctctg gagcgtttgc gggtggtgtg ttggccttgg caaggtgcgg tggcactgaa
     2881 agaacagcac ccggaaatga ctcaatatca tattattcaa aactggctgt ggctgggggc
     2941 ggttaattcg ctggaagaag cgacaacgtt aattcggaca cccgccgggt ttgatcacga
     3001 cggttataaa attctttgta agccgctgct ttccggtaac tatgaaatta ctgaacttga
     3061 tccggcgaat gaccagcgag ccagttgatt tcactgaaca acagtttacc ttgcggttgt
     3121 aaaagccgca gggtgtgcct gccatcgaac cagcacgcgc cctggtcggt agtgagcaat
     3181 ttatcgccca gttgccacgc cccgttaatc acaaacacca ccccgccgcg tgagccaaag
     3241 gtagtaaacg tacgttcggc aattctgact ttcgccttac agacatccag gcgtgtcatg
     3301 atgttgaaat ccatcgacat ctgcccttcc gtcagtttcg cttttaccac ctggtccgct
     3361 gcaaaggcaa aaggctgtaa cggttttaag gtatggttaa agcggtctgc gctttcaagg
     3421 agcatctcgc cgccttccag caacgtcact atcctttcca tgccgggaaa caaagaaaac
     3481 tccccattcg ccgcgatgga tgcaatgctg gcacgccagt aaaaatcacg ttttgccggt
     3541 gggaaagtgc aaatttcgcg cgtttcaccg gcagcatttc gccacagatt caccgacatt
     3601 ttacgcatat caaagtattc catactcgct cccggcccgt tcttttcatt gttatcgttc
     3661 aggtaaccgt ggatggttat cggcaactcg ggtgatttac gttagtggtg atcaggcttt
     3721 ttttacaact gtttgagaag agaaaagaaa accgccgatc ctgtccaccg cattactgca
     3781 aggtagtgga caagaccggc ggtcttaagt tttttggctg aaagattatt cagcagttgc
     3841 aggcatttta ccttttg
//
LOCUS       DAZPDU010000279         3846 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZPDU010000279 DAZPDU010000000
VERSION     DAZPDU010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3846
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.283"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..1361)
                     /locus_tag="ACHURS_004328"
     CDS             complement(<1..1361)
                     /locus_tag="ACHURS_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFR1239897.1"
                     /translation="MNRTSPHYCRRSVLSLLISALIYAPPVMAAFTTNVIGVVNDETV
                     DGNQRVDERGTTNNTHIINHGQQSVYGGVSNGSLIESGGYQDVGRNNNYMGQSNNTTI
                     NGGRQTIHDGGISTGTIIDSGNQDVYTGGISNGTTIKGGNSHISGGTANGTIIDGGGQ
                     TVTTQGHVDGTTINKSGYQDITQGSMATNTIINGGRQYVEQSTVGTTTIKNGGEQRVY
                     ESHALDTTIEGGTQSLNNKSTAKNTQIYSGGTQIVDYTSSSDVIEVYSGGVLDVSGGT
                     ATNVTQHDGAILKTNTNGTTVSGTNSEGAFSIHNHVADNVLLENGGHLDINAYGSANK
                     TIIKDKGTMSVLTNAKADATRIDNGGVMDVTRNATNTIINGGTQNINNHGIATGTNIN
                     SGTQNIKSGGKADTTNISTGSRQVVEKDGTATGSNISAGGSLIVYTGGIAHGVNQETG
                     SALV"
     gene            complement(1426..1545)
                     /locus_tag="ACHURS_004329"
     CDS             complement(1426..1545)
                     /locus_tag="ACHURS_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFR1239898.1"
                     /translation="MPLSYVEHHHFIMMSLMSGEHNTLALSLQDEPPGLVPAG"
     gene            complement(1769..1927)
                     /locus_tag="ACHURS_004330"
                     /pseudo
     CDS             complement(1769..1927)
                     /locus_tag="ACHURS_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239934.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2272..3750)
                     /locus_tag="ACHURS_004331"
     CDS             complement(2272..3750)
                     /locus_tag="ACHURS_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5507 domain-containing protein"
                     /protein_id="HFR1239899.1"
                     /translation="MLVSKSNEFNTSSFIDSGNCNERKSSESMELLAYSIINLICKEA
                     ASGTYRGALETLQKMMSECIYHEGNAFVIMGSGEQLKRIKYDVDENNLKVFNVYFDNN
                     EELVTDGEPDVVCLSKQVWEDLLIKLKLENKENAVSETDLSSNKNNVDQFIECAKRNE
                     QTLFDNIRKSDFHVDSFKPGSTRSVILETQPNVCMEPHNLYDNQIDKVSPVTKNSQQV
                     KGHYGDKFKEMQMFLNQMSNALQQTPSLSESKEHTIDIQKITDAFVKEFRGVLIDKNG
                     NCSERLFNFYECCYKFLPRAQPQDKIESYNSALQAFSIFRSSTLNNNDVGFNFKLFPE
                     VKLSGENLETVFKYKKGSFVREIARINITLQKEEDGLYNLGGLDFKGCFFSGQNFSNY
                     DIQYVNWGTSLFDLDTPCIFNAPAYNKSNEKSLKPVSENGLSGVLTDRNNKIKLITGV
                     APFDDILFMDDDFDDSSSEDDPVENSPVVTSPVVSSSKSSFQ"
BASE COUNT         1072 a          856 c          672 g         1246 t
ORIGIN      
        1 actaaagcac tgcccgtctc ctggttaacc ccatgtgcta taccgccggt atagacaatc
       61 agcgagcctc cggcgctaat attgctgcca gttgccgtac catctttctc aacaacctgc
      121 cggctcccgg tggaaatatt tgtcgtgtca gctttcccgc cgctcttgat attttgtgtt
      181 ccgctgttga tattggtacc cgtggcaata ccatgattat taatattctg tgtaccacca
      241 ttgattatgg tattggtcgc gtttctggta acatccatta ccccgccgtt atctattcgg
      301 gtcgcatcag ctttagcatt cgttaaaact gacattgttc ctttatcttt aataatcgtc
      361 ttatttgccg aaccatatgc gtttatgtct aaatgaccac cgttttccag caacacattg
      421 tctgccacgt gattgtggat ggagaatgca ccttcactat tcgtaccgct caccgtcgta
      481 ccgttagtgt ttgtttttaa aattgcacca tcgtgctggg taacatttgt tgccgtacca
      541 ccactaacat caagcacgcc accggaataa acttcaataa catccgagga gctggtgtaa
      601 tcaacaattt gcgtaccacc agaatagatc tgagtatttt ttgccgttga cttattattc
      661 agagactgag ttccaccttc aatcgtcgtg tccagcgcat ggctttcata tactctttgc
      721 tcaccgccat ttttaatggt ggttgttcct actgtgctct gttcaacata ctgtcgtcca
      781 ccatttatga ttgtgttcgt tgccatactt ccttgcgtga tgtcctgata accggactta
      841 tttatcgttg taccatcaac atgcccctga gttgttactg tctgccctcc accatcaatg
      901 atggttccat tcgcagtccc cccacttatg tgtgaattac cacccttaat tgtcgttcca
      961 ttgctgatac cacctgtata aacgtcctga ttgccactgt cgattatcgt accggtggaa
     1021 atgcccccgt catgaattgt ctgtcttcct ccgttaatgg ttgtattatt agactgcccc
     1081 atataattgt tattccttcc aacatcttga tatccaccag attcaataag acttccatta
     1141 gatactccgc cataaacact ctgctggcca tggttgataa tatgagtgtt atttgttgta
     1201 cctcgttcat ctactcgttg gttgccatct acagtctcat cgtttaccac accaataaca
     1261 ttagtagtaa aggccgccat cacgggcggg gcatatatca aggcagatat caataaggaa
     1321 agtactgagc ggcgacaata atggggactg gtcctgttca taaatttcat cctctgaaaa
     1381 gtgaatactg agtagcgttt aagcgacttt agctctgctg caacatcagc ccgcaggcac
     1441 cagaccgggg ggttcatcct gaagagacag cgcaagtgta ttgtgttcac cgctcatcaa
     1501 agacatcatg atgaaatgat gatgttccac ataagaaaga ggcatttttt aaacacagtg
     1561 cgctgaagtg tgattggata aaaaagtcaa tctattcagg aaatacgggc gtattctttt
     1621 ctttcgacaa tcaggccgtc ggcaaaataa aatgatttac ataatcgttt ctgatgaata
     1681 tcttctgctc acataaaaat cacacaataa ctttgagatc gcagattctt ttacttttac
     1741 agcattcgtc cccccattgt tgggcaaata tagattgggc cagagcacga aagttaatac
     1801 cacgtttgca cagctcctcc aacagcacga caagatgcca catactgcgc cccagtcggt
     1861 gcggtttaca gactacccgt gcgtccaccg ccgataatgt cctgagcagt tttttcagtc
     1921 cggacctcct gccacggttt tacgttgaag aggtaaccct gagcacgcag ttcttcagtc
     1981 aggcgtggtg caccgtaacg ctgttattgc tgggtaagat caaaaaactt tcaggcagct
     2041 aaggaaagtt gaaccagaca ttagatgaaa tatttcaacc aattacagca ccaatttaaa
     2101 ctcagtaaat cacagcacag atcaaggggt tacgtgaaag cgtagcccct ttttctttgg
     2161 tagtggcagc aaaatggttg tagtgtaaaa aataatcccg tttaatcaat caataataca
     2221 tattgtttca atctacgtta ttatctcttt gtaaaaatag ccatttatta atcattgaaa
     2281 actgctttta gaacttgata caacgggact agtcacaaca ggactattct caacgggatc
     2341 atcctcagag gaactatcat caaagtcatc atccataaat aaaatatcat cgaatggtgc
     2401 cacgcccgtg atgagtttta ttttattatt acgatcagtc aagactccac ttaaaccgtt
     2461 ttcgctcaca ggttttaatg atttttcatt actcttgttg taagcaggcg cattaaaaat
     2521 acacggagta tcaagatcaa acaatgacgt tccccagttc acatattgaa tatcatagtt
     2581 actgaagttc tgtccagaaa agaagcatcc cttaaaatcc aatccaccta aattatataa
     2641 accatcctct tctttttgga gagtaatgtt aattctggct atctcccgga caaaacttcc
     2701 ttttttgtat ttgaataccg tttcaagatt ttcaccagac agcttgactt ctgggaataa
     2761 tttaaaatta aaccctacat cattattatt caaagtagat gaacgaaaga tggaaaaagc
     2821 ttgtagtgct gaattatagc tttcgatttt atcttgaggc tgcgctcttg gtaaaaactt
     2881 atagcagcac tcataaaaat tgaataaacg ttctgaacaa ttcccatttt tatcaattaa
     2941 taccccccgg aattctttca caaaggcatc cgttattttt tgaatatcta tggtgtgttc
     3001 ctttgactct gacaaagatg gagtctgttg aagtgcattg ctcatctggt taaggaacat
     3061 ctgcatttct ttaaatttat ctccatagtg gcctttaact tgctgagagt ttttagttac
     3121 aggtgaaacc ttatctattt ggttgtcata taaattatgt ggttccatac agacatttgg
     3181 ttgtgtttct aaaataacac tacgtgtact acctggctta aatgaatcaa catgaaaatc
     3241 actttttctt atattatcga atagggtctg ttcatttctc ttagcgcatt caataaactg
     3301 atcaacatta tttttattcg atgacaggtc agtttcagaa accgcatttt ctttgttctc
     3361 cagctttagt ttaatgagaa gatcttccca gacctgcttg cttaaacata ctacgtcagg
     3421 ctcaccatca gtaactaact cttcattatt atcaaagtat acgttgaata cttttaagtt
     3481 attttcatca acatcatatt taatacgttt taattgctct ccagatccca taatgacaaa
     3541 ggcgttgcct tcatgatata tacattcaga catcattttt tgtaaagttt caagagcacc
     3601 gcgatacgtc cctgaggcag cttccttaca aattaaattt ataatactat aagctagtag
     3661 ctccatggat tcagaagatt ttctttcatt acaatttcca ctatctataa atgagctagt
     3721 gttaaattcg ttgcttttac taactaacat tgtctattcc tcagttaatg tctacatggc
     3781 tatttttaat tttattattg tttgtcacta taaaaaatcg ctcatttgag acaattgctg
     3841 gcatta
//
LOCUS       DAZPDU010000280         3833 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZPDU010000280 DAZPDU010000000
VERSION     DAZPDU010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.284"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..266)
                     /locus_tag="ACHURS_004332"
     CDS             complement(<1..266)
                     /locus_tag="ACHURS_004332"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFR1239900.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRELTDAGGFLDISL"
     gene            353..760
                     /gene="nudG"
                     /locus_tag="ACHURS_004333"
     CDS             353..760
                     /gene="nudG"
                     /locus_tag="ACHURS_004333"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFR1239901.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFADGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            complement(726..998)
                     /locus_tag="ACHURS_004334"
                     /pseudo
     CDS             complement(726..998)
                     /locus_tag="ACHURS_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
     gene            1234..2577
                     /gene="gdhA"
                     /locus_tag="ACHURS_004335"
     CDS             1234..2577
                     /gene="gdhA"
                     /locus_tag="ACHURS_004335"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFR1239902.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(2694..3734)
                     /locus_tag="ACHURS_004336"
     CDS             complement(2694..3734)
                     /locus_tag="ACHURS_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR1239903.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMTSISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
BASE COUNT          999 a          932 c          916 g          986 t
ORIGIN      
        1 tcaagagaaa tatcaagaaa gccgcccgcg tcggtaagct ctctgcgccg cgccagcgca
       61 ccgtccagac catcaagcag cctgttcaac aaaatgacga ctagcgccgc cagataccag
      121 cccagcgcca gaaacggcag agccagcacg ccgatggcaa atcccactaa tgttaaacca
      181 tctggcgtaa tgcccggttt atcaagaacc cgcacgcact gatgcaacaa cggtttaatc
      241 cggggatgaa gatggcggtc tagcacaggc actccttaaa tataaagcct ttctgattga
      301 gcaacagtgc ggatattatg gcatttttcg cttatctgcc cgtgtgtaat ttatgaaaat
      361 gattgaagtt gttgccgcca tcattgaacg tgatggcaaa attttactcg cgcaacgccc
      421 cgcccagagc gatcaggcgg gattatggga gtttgccgat ggtaaagtcg agctggatga
      481 aagccagcag caggcactgg tgcgtgagtt aaacgaagaa ttgggcatcg aagcaactgt
      541 gggtgagtat gttgccagcc atcagcgaga agtttcgggc cggattatcc atcttcatgc
      601 ctggcacgta cccgacttcc acgggacgtt acaggcacat gaacatcagg cgctggtctg
      661 gtgctcacct gaagaggcgc tgcaatatcc gctggcccct gctgacattc cactattaga
      721 ggcgtttatg gctttacgcg ccgccagagc agcggattag tgctaagggt tttgtcatcc
      781 cgctggcatt acagcagtat tccttcggct ttaattaccg ccccttcaga ataattttga
      841 tcctgataaa cgcagcactg agtacagggc tgcgctgact gcccgcctga actgaacact
      901 tctggcggta cgtttacctc cacgtccgga cgataatgcg ggttagccag tgcaattaat
      961 ggaaatgcta atactacggc gaacaatgct cgactcacag ggaactcctt aacgttattg
     1021 tctctgcaac tgataacggt agccgggtgg caaaacttta gcgtctgagt tatcgcattt
     1081 ggttatgaga ttactctcgt tattaatttg ctttcccggg tcattttttt cttgcttacc
     1141 gtcacattct tgatggtata gtcgaaaact gcaaaagcac atgacataaa caacataagc
     1201 acaatcgtat taatatataa gggttttata tctatggatc agacatattc tctggagtca
     1261 ttcctcaacc atgtccaaaa gcgcgacccg aatcaaaccg agttcgcgca agccgttcgt
     1321 gaagtaatga ccacactctg gccttttctt gaacaaaatc caaaatatcg ccagatgtca
     1381 ttactggagc gtctggttga accggagcgc gtgatccagt ttcgcgtggt atgggttgat
     1441 gatcgcaacc agatacaggt caaccgtgca tggcgtgtgc agttcagctc tgccatcggc
     1501 ccgtacaaag gcggtatgcg cttccatccg tcagttaacc tttccattct caaattcctc
     1561 ggctttgaac aaaccttcaa aaatgccctg actaccctgc cgatgggcgg tggtaaaggc
     1621 ggcagcgatt tcgatccgaa aggaaaaagc gaaggtgaag tgatgcgttt ttgccaggcg
     1681 ctgatgaccg agctttatcg tcatctgggc gcggataccg acgttccggc aggtgatatc
     1741 ggcgttggtg gtcgtgaagt cggctttatg gcggggatga tgaaaaagct ctccaacaat
     1801 accgcctgcg tcttcaccgg taagggcctt tcatttggcg gtagtcttat tcgcccggaa
     1861 gctaccggct acggtctggt ttatttcaca gaagcaatgt taaaacgcca cggtatgggt
     1921 tttgaaggga tgcgcgtttc cgtttctggc tccggcaacg tcgcccagta cgctatcgaa
     1981 aaagcgatgg aatttggtgc tcgtgtgatc actgcgtcag actccagcgg cactgtagtt
     2041 gatgaaagcg gattcacgaa agagaaactg gcacgtctta tcgaaatcaa agccagccgc
     2101 gatggtcgag tggcagatta cgccaaagaa tttggtctgg tctatctcga aggccaacag
     2161 ccgtggtctg taccggttga tatcgccctg ccttgcgcca cccagaacga actggatgtt
     2221 gacgccgcgc atcagcttat cgccaatggc gttaaagccg tcgctgaagg ggcaaatatg
     2281 ccgaccacca tcgaagcgac tgaactgttc cagcaggcag gcgtactgtt tgcaccgggt
     2341 aaagcggcta atgctggcgg tgtagcaacg tcgggcctgg aaatggcaca aaacgctgcg
     2401 cgcctgggct ggaaagccga gaaagttgac gcacgtttgc atcacatcat gctggatatc
     2461 caccatgcct gtgttgagca tggtggtgaa ggtgagcaaa ccaactacgt gcagggcgcg
     2521 aacattgccg gttttgtgaa ggttgccgat gcgatgctgg cgcagggtgt gatttaagtt
     2581 gtaaatgcct gatggcgcta cgcttatcag gcctacaaat gggcacaatt cattgcagtt
     2641 acgctctaat gtaggccggg caagcgcagc gcccccggca aaatttcagg cgtttatgag
     2701 tatttaacgg atgatgctcc ccagggaaca tttcttatgg gccaacggca tttcttactg
     2761 tagtgctccc aaaattgctt gtcgtaacga taacacgctt caagttcagc atccgttaac
     2821 tttctgcgat agcagcagat atgccagtaa agaaacccca ttagactatt tttttgataa
     2881 tcttcttcgc tttcgaacaa ctcgtgcgcc tttcgagaag caagcattat ataatgccag
     2941 gccagttctt cttcaattgt cccgttttga aaagctgtgc ttgatatcga ggtcatccat
     3001 gataattccg ccgcccatat tagcttcgcc gaggatttac cggagcgatt attagcgcaa
     3061 tcagagatat agtctgaggg aaaaacagca aatttattca acaaggcgat aacctgctct
     3121 ggggcttcct ccatgtttgc tttaaaggta ttggctccat ggtcgccaga aagaaaatgc
     3181 tccattaagg cacaataact ttcgctatct tcgatacccc attgattctc taaagactcg
     3241 cgtcttttac tgatgatatc gatcgagtca aaaggaagca catgatattg gaaggtatct
     3301 ttaccaggtt caggctttcg cggccagaac tctagcgttt cagaccattg cttatgatag
     3361 aatcgataag gtgcgatcaa ttgtagcgcc tgtaacttct cgatactgag cggctcaata
     3421 cctttagcct gataataatg cagttgttct ttttttgctt taaaaccggc ccgaacaata
     3481 agccccatca taattaatag ataaagaaaa gagcatcccg cggtaatcag gcctctttca
     3541 ttcaaaccgt tggatgttat cgctgcgaac acaaacatta cagcgacaac acatgttaaa
     3601 taaaaccccc acttacaaag cagcatggcc ttattttctt taatcatccg ttcaaaatta
     3661 ctattaaata tttcccagcc attaaaagaa tacttctcgc tcccaggatg gttttgtaat
     3721 aaaacttttt tcatcacatt ccctgttatt acatacttta gaggcgataa ttttatccat
     3781 gcaaaaaaaa tatccaacga attttcttga tccgctggat atttcagaat atg
//
LOCUS       DAZPDU010000281         3828 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZPDU010000281 DAZPDU010000000
VERSION     DAZPDU010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3828
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.285"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..197
                     /locus_tag="ACHURS_004337"
     CDS             <1..197
                     /locus_tag="ACHURS_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239904.1"
                     /translation="SPKKIGFYTIGDKSIYLYDLRRMDEIMEALDNRSSMDWCVAVHD
                     MNAGFDEKILFPSSVESTAG"
     gene            208..375
                     /locus_tag="ACHURS_004338"
     CDS             208..375
                     /locus_tag="ACHURS_004338"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239905.1"
                     /translation="MTTITKERIELFIKNPLENGLTRGEQMELARIALASLEAEPVVF
                     WFEKYQEGATA"
     gene            372..716
                     /locus_tag="ACHURS_004339"
     CDS             372..716
                     /locus_tag="ACHURS_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239906.1"
                     /translation="MTTFTREQLIAHAEETIEAQRLCIPGTIDHDIIRTYKMDIAVLE
                     IALVSLAAEPAGKLHEYKPVGYQRLVDELTMLVKQLTWQLRKAKPDCKLPDKAMSYLE
                     RNGLISVEDILR"
     gene            1008..1163
                     /gene="hokD"
                     /locus_tag="ACHURS_004340"
     CDS             1008..1163
                     /gene="hokD"
                     /locus_tag="ACHURS_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR1239907.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1384..1672
                     /locus_tag="ACHURS_004341"
                     /pseudo
     CDS             1384..1672
                     /locus_tag="ACHURS_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1711..1989
                     /locus_tag="ACHURS_004342"
     CDS             1711..1989
                     /locus_tag="ACHURS_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239908.1"
                     /translation="MAQNSRLHNSDNSAVFASRHGRRSHAFKSDWYQHPPCTEEQAEW
                     LIQCYRRRGCEVKKALSLDYRHWIISVRLPYSERPTRPSRTFQQRIWR"
     gene            1991..3049
                     /locus_tag="ACHURS_004343"
     CDS             1991..3049
                     /locus_tag="ACHURS_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFR1239909.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMSAFHNGRILVEPEPKSMRN
                     LPSGVVPAARQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSVYSESDIIPGEQTATSILKQR
                     TKNIALLPHVHQQQNPPQEKTVVSIAVDPESPAQYLQRQKSQREEMPVYTRWVKTQKC
                     MTCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCRNELHAGVKDFEEKHGSQLLL
                     LIRFLMHARNSGVLKWKA"
     gene            3050..3430
                     /locus_tag="ACHURS_004344"
     CDS             3050..3430
                     /locus_tag="ACHURS_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR1239910.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVR
                     QQRLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVR
                     AQPVSGGRLGVKIYPIMLEGQVKK"
     gene            3427..>3828
                     /locus_tag="ACHURS_004345"
     CDS             3427..>3828
                     /locus_tag="ACHURS_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR1239911.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGVSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNK"
BASE COUNT         1034 a          853 c         1046 g          895 t
ORIGIN      
        1 catcaccgaa aaaaattggc ttttacacca ttggcgataa aagcatttac ctgtatgacc
       61 tacgccgcat ggatgaaatc atggaggctc ttgataatcg ttcgtcgatg gattggtgtg
      121 ttgctgttca tgatatgaat gcagggtttg atgaaaagat tttgttcccc tcatcagttg
      181 aaagcactgc gggttaagga gtaacacatg accactatta ccaaagaacg tattgaattg
      241 ttcattaaaa acccgcttga aaacgggctt acccgtggtg aacaaatgga actggcacgg
      301 attgcgctgg catcgctgga agcagagccg gttgtgttct ggtttgaaaa atatcaagaa
      361 ggggctacgg catgacgact tttaccagag agcagttaat agctcacgca gaggagacta
      421 ttgaagcaca gagactgtgc ataccgggca caatcgacca tgacatcatc cgcacatata
      481 agatggatat tgctgttctg gaaatcgcac tggtatcgct ggcagcagag ccagccggta
      541 aattgcatga atacaaacca gtgggatatc agcgtctggt cgatgagtta accatgctgg
      601 taaagcagtt aacctggcaa ctgagaaaag cgaagccaga ctgcaaatta ccggataagg
      661 cgatgagtta tctggagcgg aacggactga taagcgtgga ggatatttta cgatgacctg
      721 gcctgaagca ttaacaacgg taggaattgc gatggcggtg gcgctggtgg tgtattcgat
      781 ttgccgctgg ggataaaaac ggtttgcggg aaaaggagag ttaagtagaa ttgcagcggg
      841 tgcttgaggc tatctgtctc aggcatgaac accaaaaggc agatagagaa aagccccagt
      901 taacattacg cgtccggcaa gacgcttaac attaatctga ggctcaatct atgaacggca
      961 aatctaggtt agcctcttac gtgccgaaag gcaaggagaa gcaggctatg aagcagcaaa
     1021 aggcgatgtt aatcgccctg atcgtcatct gtttaaccgt catagtgacg gcactggtaa
     1081 cgaggaaaga cctctgcgag gtacgaattc gaaccggcca gacggaggtc gctgtcttca
     1141 cagcttacga acctgaggag taagagacca ggcgggggag aaatccctcg ccacctctga
     1201 tgggtcaggc atcctcaatg cacccacact taacccgctt cggcgggttt ttgtttttat
     1261 tttcaacgcg tttgaagttt tagatggtgc cggaatagaa tcaaaaatac ttaagtagcg
     1321 cgcagggaga agagggatgg accccgaaca ggggagtgct atttatctgg aaggattctg
     1381 ttgatgaaaa tcgaagaatt acgtgaaatt tttagtgaag atggcctcta tactgtgcgc
     1441 gttgagaatg gcgctattgt cagccactgc cgtattaaat gtttacagtc tcaacaaagg
     1501 aagagtggag ctgcgttaat tcattttgtg gatgggcttg tgacggatgg ttttatttcg
     1561 cgtgcaaatg aatttgtcac atcgttgccg tctctgaaag aagctgggat taaggctggt
     1621 ttttctgctt ttgaagatga gtgaattcat ctacaattca gcgcagggct gaacccctgt
     1681 tgagtaacac tgtgccaccg gagaaagccg atggcgcaaa attccagact acacaattct
     1741 gataattcag ccgtctttgc cagcaggcac gggcggcgct ctcacgcatt taaatctgat
     1801 tggtaccagc atcccccatg cactgaagaa caggccgaat ggctcattca gtgttaccgc
     1861 aggcgcggat gcgaggttaa aaaagccctt agcctcgact accgtcactg gataatctcc
     1921 gtcaggctcc cttactccga acggccaacg cgtccgtccc gcacattcca gcaacggatc
     1981 tggaggtaat gtgcgggtat tacttcgacc tgttctggta ccggaactcg gtctggttat
     2041 cgttaagcca ggccgtgaat caatgtcagc attccataac ggcagaatac tggtggagcc
     2101 ggaaccaaaa agcatgcgta atctgccgtc cggggtcgtt cctgccgctc gccagccgct
     2161 ggtggaagac aaaacattgc tgccgttttt cagtaacgca cgggtgattc gtgctgctgg
     2221 tggtgctggt gcattgtctg actggctgtt gcgccatatt aaatcctgcc agtggccaca
     2281 cggcgattat catcacagcg aaaccgtcat tcaccgttat ggtaccggcg caatggtgtt
     2341 gtgctggcac tgcgacaacc agctgcgtga ccagacatcc gaatcactcg agcaacttgc
     2401 tcatcaaaac ctgtcagcat ggatgattga cgtcatcggt cacgcaataa gcggtacgca
     2461 ggagcgtgaa ttatctctgg ctgaattatc ctggtgggcg gtccgcaatc aggtggcgga
     2521 cgcgctaccg gaagcggtat tacgtcgttc gctggggttg cgtgcggaaa aaattcgctc
     2581 tgtgtacagt gaaagcgaca tcataccggg agagcagaca gccaccagca tactgaagca
     2641 gcgcacaaaa aatattgcgt tactgcctca cgtccaccag caacagaacc caccacagga
     2701 aaagacggtg gtcagcattg ccgttgatcc ggagtcaccg gctcagtatc tccagcgcca
     2761 gaaatcacaa cgggaagaga tgcctgtata cacgcgctgg gtaaaaacgc agaaatgcat
     2821 gacgtgtggc aatcaggcag atgatccgca tcacatcatt ggtcatggac tgggagggat
     2881 gggaacaaag gctgatgatt tgtttgttat tccgttatgc cgtaaatgcc gtaacgaact
     2941 acatgccgga gtaaaagatt ttgaagagaa acacggcagt cagctgttgt tgctgattcg
     3001 ttttttaatg cacgcgagaa attcgggtgt tctgaagtgg aaagcataaa tgaccgagcg
     3061 catagaattt gttttacctt acccgccgac ggtgaacact tactggcgtc gtcgtggcag
     3121 cacatatttt gtatcaaaag ccggggagcg ttatcgccgg gcagtggcgc ttattgttcg
     3181 ccagcagcgg ctgaaattaa gcctgtccgg aaggttggca ataaaaatta ttgcagaacc
     3241 accggataag cgccgccgtg acctggacaa tattctgaaa gcgccgctgg atgcgctgac
     3301 gcatgcgggg ttgctaatgg acgatgagca gtttgatgaa atcaatatcg ttcgtgctca
     3361 gccagtatct ggtggacgtc tgggggtgaa gatttatccc ataatgcttg aagggcaggt
     3421 caaaaaatga aactggaaga tttaccgaaa tactactccc caaaatcccc cggcctgact
     3481 gatgcatcgg cctcaacgtc gaaagatgcg ctgagtatca ctgatgtgat ggccgcgcag
     3541 ggcatgacac agaatcgggc tgagatgggg gtttctgcgt tccttgggaa aatgggcatt
     3601 agtatgaatg acagagagcg ggcaacagaa ttgctgacag aatatgcact cagtcggtgt
     3661 gatcgcgtgg cggcgttaag aaaactcccg gcagaaataa aaccggcagt gatgcgtatt
     3721 atggcttcgt atgcgtttga agattatgcc cgtagcgcgg cgagcaaaaa acagtgcccc
     3781 tgctgtcacg gaaaaaaatt tattgaaagc gaggttttta caaacaag
//
LOCUS       DAZPDU010000282         3797 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZPDU010000282 DAZPDU010000000
VERSION     DAZPDU010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.286"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..81
                     /locus_tag="ACHURS_004346"
     CDS             <1..81
                     /locus_tag="ACHURS_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239912.1"
                     /translation="REWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(98..907)
                     /gene="proC"
                     /locus_tag="ACHURS_004347"
     CDS             complement(98..907)
                     /gene="proC"
                     /locus_tag="ACHURS_004347"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFR1239913.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            1027..1485
                     /locus_tag="ACHURS_004348"
     CDS             1027..1485
                     /locus_tag="ACHURS_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFR1239914.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            1668..2192
                     /gene="aroL"
                     /locus_tag="ACHURS_004349"
     CDS             1668..2192
                     /gene="aroL"
                     /locus_tag="ACHURS_004349"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFR1239915.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            2242..2433
                     /gene="yaiA"
                     /locus_tag="ACHURS_004350"
     CDS             2242..2433
                     /gene="yaiA"
                     /locus_tag="ACHURS_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFR1239916.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            2691..3368
                     /locus_tag="ACHURS_004351"
     CDS             2691..3368
                     /locus_tag="ACHURS_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFR1239917.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLTVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            3440..3724
                     /gene="ppnP"
                     /locus_tag="ACHURS_004352"
     CDS             3440..3724
                     /gene="ppnP"
                     /locus_tag="ACHURS_004352"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFR1239918.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
BASE COUNT          951 a          903 c          990 g          953 t
ORIGIN      
        1 cgtgagtggt tgaaatcggc agatttggcg ctttacaaag caaagaaagc cggacgtaac
       61 cgcaccgaag tggcggcctg acgtccggcg aaagtcatca ggatttgctg agtttttctg
      121 atttttccat acacttcgtc atcgcttcga tcactgcggc acggaagcct ttctcttcca
      181 gtacgcgtac cgcttcaatg gtggtgcctc ccggtgagca gaccatatct ttcagtgccc
      241 ccggatgttc tcccgtttcc agcaccattt ttgcggaacc cattaccgcc tgagcggcaa
      301 atttatacgc ctgggcgcgt ggcatcccgc ccagcacggc ggcgtcggcc atcgcttcga
      361 taaacataaa tacgtaggct ggcgaagaac cgctcacacc gaccaccggg tggatcatcg
      421 gctcagcaat tacttccgct tcgccaaagc agcggaaaat attcagcaca tcagcggtat
      481 cttcgggggt taccagcgcg tttggcgtta cggaggtcat cccggcatta accagcgcgg
      541 gagtgttcgg catggcgcgg ataattttcc ggtcatggcc cagcgcgcgg gcaagctggt
      601 cgagcgtgac acctgcagca atagaaacga ccagagagtc tttattcagg ctggaggtga
      661 tttcgctaag cactttaatc atgatgccag gtttaacggc ggcaaaaatg atgtcggcga
      721 tttgcgccac ttcttgcgcc gattctgcgg cgttgatgcc gaactggtca tgcagggcgg
      781 cgactttatc cggggagggg gtgtataccc agatttgccc tggaagcacc tgaccgctgg
      841 caatcagacc gccgagaatg gcttttccca tattgccgca gccaataaaa ccgattttct
      901 tttccattgc ctcactcctg ccgtgaaatt cattgttttg ataatcgctg gcagaagcat
      961 aaacagaact atgccggaag gcaaaagcgc gacacaatag aggattaccc aacaaaggat
     1021 gactttatga caatttgggt ggatgccgac gcgtgtccca atgtaattaa agagattttg
     1081 tatcgcgcgg cggaacgtat gcagatgccg ctggtactgg tagcaaacca gagtttacgc
     1141 gtgccgccat cgcgatttat tcgtacgctg cgcgtcgcgg caggtttcga cgttgccgat
     1201 aacgaaattg tccggcagtg tgaagcgggc gatttggtga tcaccgcaga tatacctttg
     1261 gctgctgaag ccatcgagaa aggcgcagcg gcgcttaatc cgcgcggcga acgttacacg
     1321 ccagcgacca ttcgtgagcg cctgacgatg cgcgatttta tggatacctt acgtgccagt
     1381 gggatccaga ccggcggacc agatagcctt tcacaacgtg accgccaggc ctttgccgcg
     1441 gagctggaga agtggtggct ggaagtgcaa cgtagtcgtg gctaaatgta atttattatt
     1501 tacacttcat tcttgaatat ttattggtat agtaagggat gtattgagat tttcacttta
     1561 agtggaattt tttctttaca atcgaaattg tactagtttg atggtatgat cgctattctc
     1621 atgacaccgg ctttcgccgc attgcgacct attggggaaa acccacgatg acacaacctc
     1681 tttttctgat cgggcctcgg ggctgtggta aaacaacggt cggaatggcc cttgccgatt
     1741 cgcttaaccg tcggtttgtc gataccgatc agtggttgca atcacagctc aatatgacgg
     1801 tcgcggagat cgtcgaaagg gaagagtggg cgggatttcg cgccagagaa acggcggcgc
     1861 tggaagcggt aactgcgcca tccaccgtta tcgctacagg cggcggcatt attctgacgg
     1921 aatttaatcg tcacttcatg caaaataacg ggatcgtggt ttatttgtgt gcgccagtat
     1981 cagtcctggt taaccgactg caagctgcac cggaagaaga tttacggcca accttaacgg
     2041 gaaaaccgct gagcgaagaa gttcaggaag tgctggaaga acgcgatgcg ctatatcgcg
     2101 aagttgcgca tattatcatc gacgcaacaa acgaacccag ccaggtgatt tctgaaattc
     2161 gtagcgccct ggcacagacg atcaattgtt gattttagag cgcctatact taacgttcat
     2221 cccgtgaaat aaggaagaac gatgccaacg aaaccgcctt atcctcgtga agcatatata
     2281 gtgacgattg aaaaaggaaa gccaggacag acggtaacct ggtaccaact cagagccgat
     2341 catcctaaac cagactcgtt gatcagtgaa catccgacgg ctcaggaagc gatggatgcg
     2401 aaaaaacgct atgaggaccc tgacaaagag tgaccgcatc agactgctcg gaagggattc
     2461 tgagtgccac tacaagggat ctgcgtcaca tttttcataa ttcatgtttt tctaataatt
     2521 agaatattaa acaataacaa tccattactg gaatcatttg gaatctttac attatgccgt
     2581 gcacgtctgc tgctacgctt tttgtcattt gtagcacaag taagtgtcag cagtggtgct
     2641 tcacacttgc ccggtaatta acgacgaaag aaaagtaagg tggatgaaca atgagtgcgt
     2701 cgttggcgat cctgaccatc ggcattgtac ctatgcagga ggttttgccg ctcctgacgg
     2761 aatacattga cgaagataat atttcccatc atagcctgct ggggaagtta agtcgtgaag
     2821 aagtgatggc ggagtacgcg ccagaagcag gcgaagacac cattctcaca ttattaaatg
     2881 acaaccagct ggcccatgtt tcgcgtcgta aagtggagcg agacctgcaa ggtgtggttg
     2941 aagtgctcga taatcagggt tatgacgtca ttttattaat gagtacagca aacattagta
     3001 gtatgactgc gcgtaatacg atctttcttg agccgtcgcg aatattgcct ccactggttt
     3061 cctctattgt tgaagatcat caggtggggg ttatcgttcc ggttgaggag atgctgaccg
     3121 ttcaggcgca aaaatggcaa attttgcaga aatcgccggt attttcattg ggtaacccca
     3181 ttcatgattc agaacaaaaa atcattgatg ccgggaaaga attactggca aaaggggctg
     3241 atgtcatcat gctggattgt ttaggatttc accaacgtca tcgcgattta ctgcaaaaac
     3301 agctcgatgt tcctgtcttg ctgtctaacg tattgattgc acggctggct gcggaattac
     3361 tggtgtaatt ttgcgtgaca gccagcgcct ctggccccta tagtgaagta gatgttcaac
     3421 taccagacag ggccagttta tgcttcaaag taatgagtac ttttccggca aagtgaaatc
     3481 aatcggcttt tccagcagca gcactggtcg cgccagcgtg ggtgttatgg ttgaaggcga
     3541 atacaccttc agcaccgctg agccggaaga gatgacggta atcagtggcg cgctgaatgt
     3601 gttactgcct gacgcgaccg actggcaggt gtatgaagcc ggttcggtgt ttaatgttcc
     3661 cggtcacagt gagtttcatc tgcaagttgc cgaacccacc tcttatctgt gccgctatct
     3721 gtaattcctc gccttcccct tgaacgggag ggcatttttc tgaaatatcc tctctttaac
     3781 ccataataat atttcct
//
LOCUS       DAZPDU010000283         3753 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZPDU010000283 DAZPDU010000000
VERSION     DAZPDU010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.287"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..1359
                     /gene="dcuC"
                     /locus_tag="ACHURS_004353"
     CDS             <1..1359
                     /gene="dcuC"
                     /locus_tag="ACHURS_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFR1239919.1"
                     /translation="GVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAIMGHKVLPSS
                     QASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMVVKLASKPLQ
                     YINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRGAAAAICASP
                     AAIILAPTSGDVVLAAQASEVSLIDFAFKTTLPISIAAIIGMAIAHFFWQRYLDKKEH
                     ISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVICMLIASILE
                     FLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQSLISIATSF
                     GSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAYLTIPMLQAS
                     NLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELMVPGTAAAVT
                     GK"
     gene            complement(1400..2080)
                     /gene="dpiA"
                     /locus_tag="ACHURS_004354"
                     /gene_synonym="citB"
     CDS             complement(1400..2080)
                     /gene="dpiA"
                     /locus_tag="ACHURS_004354"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFR1239920.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISCTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2049..3707)
                     /gene="dpiB"
                     /locus_tag="ACHURS_004355"
     CDS             complement(2049..3707)
                     /gene="dpiB"
                     /locus_tag="ACHURS_004355"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFR1239921.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHLNPEKIGYPMQFTKQGALEKGESYFITGKGAMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLNVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
BASE COUNT          857 a          983 c          911 g         1002 t
ORIGIN      
        1 ggggttgtgg ttattgtggg tgtagctcgc tacatcatta aagggtattc cgccactggt
       61 gtgttatttg tcggtggcct gttattgctg attatcagtg ccattatggg gcacaaagtg
      121 ttaccgtcca gccaggcttc aacaggctac agcgccacgg atatcgttga atacgttaaa
      181 atattactaa tgagccgcgg cggcgacctc ggcatgatga ttatgatgct gtgtggattt
      241 gccgcttaca tgacccatat cggcgcgaat gatatggtgg tcaagctggc gtcaaaacca
      301 ttgcagtata ttaactcccc ttacctgctg atgattgccg cctattttgt cgcctgtctg
      361 atgtctctgg ccgtctcttc cgcaaccggt ctgggtgttt tgctgatggc aaccctattt
      421 ccggtgatgg taaacgttgg tatcagtcgt ggcgcagctg ctgccatttg tgcctccccg
      481 gcggcgatta ttctcgcacc gacttcaggg gatgtggtgc tggcggcgca agcttccgaa
      541 gtgtcgctga ttgacttcgc cttcaaaacg acgctgccta tctcaattgc tgcaattatc
      601 ggcatggcga tcgcccactt cttctggcaa cgttatctgg ataaaaaaga gcacatctct
      661 catgaaatgt tagatgtcag tgaaatcacc accactgctc ctgcgtttta tgccattttg
      721 ccgttcacgc cgatcatcgg tgtactgatt tttgacggta aatggggtcc gcaattacac
      781 atcatcacta ttctggtgat ttgtatgctg attgcctcca ttctggagtt cctccgcagc
      841 tttaataccc agaaagtttt ctctggtctg gaagtggctt atcgcgggat ggcagatgcg
      901 tttgctaacg tggtgatgct gctggttgcc gctggggtat tcgctcaggg gcttagcacc
      961 atcggcttta ttcaaagtct gatttctatc gctacctcgt ttggttcggc gagtatcatc
     1021 ctgatgctgg tattggtgat tctgacaatg ctggcggcag tcacgaccgg ttcaggcaat
     1081 gcgccgtttt atgcgtttgt tgagatgatc ccgaaactgg cgcactcttc cggcattaac
     1141 ccggcgtatt tgactatccc gatgctgcag gcgtcaaacc tgggtcgtac cctatcaccc
     1201 gtttctggcg tagtcgttgc ggttgccggg atggcgaaaa tctcaccatt tgaagtcgta
     1261 aaacgcacct cggtgccggt gcttgttggt ttggtgattg ttatcgttgc tacagagctg
     1321 atggtgccag gaacggcagc agcggtcaca ggcaagtaaa tagtaatgcc ggaggcaagt
     1381 ttcctccggc tttatcatgt cacccactgt ggtatatgcg ttgtggtctg ccaactttgc
     1441 cgtgaacaat ttcagcaata atcagatggc ggctggcgca atattcaaga taacgcctgg
     1501 cagtggtgca gctgatggtc agtgcctgcg ccaccgtttc tgccgtatgt tgcacaccag
     1561 gctctttaaa cagttttcgc accgcgttta gcgtcaaggg gtcaatgccg gtcggtagct
     1621 cgtccttagg ttcaccgcga gcataagcat taaacatctc atcaatttgc ttctggctgg
     1681 cgctatcaat actttccagc atatgtttac gctggcggaa acgggttagc gtttgcccca
     1741 gccgttcata ggcaatgggc ttaatgagat aatcaaatac accacaacgt acggcttcag
     1801 acaccgtttc catatcgctg gctgcagtgg taaacaccac gtcgccggga taatgcgcct
     1861 gcaccagttc atgcagtaaa ttaatccctc taccgtcagg aagatagtta tcgagcaaga
     1921 ttagccccgg cttaaaacgc tcgatcatca ttcgggcctg cgccagattt cccgccagta
     1981 atatctgact gaatccggga atgtgacgaa tatattccgc atgcatctct gccagcggcg
     2041 tttcgtcctc aacgatcaat agggttaatg gagctgtcat taggtttcac tttcggaata
     2101 tagattgaaa ataaggtacc gcagggatca ttatcttcga gagtgataac accaccgcag
     2161 cgcgttacgt agctggcaat caagtacaac ccaatgccat gttcaccggg ctcgtcagca
     2221 cgcgtactga ccccctgctc aaatattttg tctcttagag actctggaac gccgcagccc
     2281 tgatcggcga cttcaatcac cacatcatcg ccttcatcgc tgaggaataa ttcaacgatc
     2341 ttgtttcctt catcgctacg caggctggct tcgaaggcgt tatcaagtaa attgccaaca
     2401 atggcggcaa actcggtgct atccagtcct ggcggcagtt gcgaaagctg gctaccgggg
     2461 acgatgatca tttttagccc cagttcgcgg gcgcgctgca ctttaccaaa aagcagcccc
     2521 gccacctggc gatcggcaaa cgcctcacgc aggctgtcaa taagctgttg ctgggcctga
     2581 gactccccct gcaccatggc cagcacgcga tcatactctt tcatctgcaa cagaccattg
     2641 agcgtcgaca tccaattgag atgctcgtgt cgcaatgtac gaaggctctc gacgtattgt
     2701 tttatttgcg tcagttgcgc attgagggtg gaaatttcgt ctttactacg aaagctgata
     2761 atggccccca gcaaatcatc acctgaacga atagcttccc ggttggcaat aacgttcaga
     2821 ccattaaagt tcgccaccac atcctgacgt ttttcatcaa tctgttcggt aaagaaatcg
     2881 gcgggcctga ccacttcagc aatgggttta cccaaccatt gccgtccggg agagctcagc
     2941 cccagcatct ttcttgcgtt acgattgatg gcggtaatgt aaccatgcgg atccaccgca
     3001 atcagccctt cataaaccga actaaacagc gcctcttgct gacggaccac gcgtgcgatt
     3061 tgctttggct ccatgcccat catttgtcga cggatatgcg cggccaggaa ccacgacagc
     3121 aacatcagaa tccctaacag cacgacaaac acgcctgcca tcggtaataa aaactcagcc
     3181 cgccagctat cgattttact caccaggtag ccaatcgaca ccacgccgat gacttttcca
     3241 tcgtcatcaa agattggcgt tttggcgcgc atcgccatcc ccattgcccc tttcccggta
     3301 atgaagtagc tctccccttt ctccagcgcg ccctgtttgg tgaactgcat aggataacca
     3361 attttctccg gattaagatg gtaaaggcgg atcgagtgcc ggtcgccaat caccacataa
     3421 tcaaaatcgg tatctctttg taatttgtta gcgatggtcg ccagccgttt gtagtcacgc
     3481 gttttcaccg cagagatgac actgtcattg gaggcaataa ttttcgcctg attcattgcc
     3541 atgtcgcgta catgaagcgt taaatagtcc tcaaaactgg ccgtaaaata ttgcgccagg
     3601 gctgcaatga caaatattga gaacaccaga atcagcaaaa agatacgcag cggaaatgcc
     3661 agtctttgga aaaatgcaaa ctgtttattc tcgttaagct gcaacattgt tttccttgcg
     3721 ggttgtcccg agcctctttg acggatgtaa tta
//
LOCUS       DAZPDU010000284         3734 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZPDU010000284 DAZPDU010000000
VERSION     DAZPDU010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.288"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..182)
                     /locus_tag="ACHURS_004356"
     CDS             complement(<1..182)
                     /locus_tag="ACHURS_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HFR1239922.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSI"
     gene            426..683
                     /locus_tag="ACHURS_004357"
     CDS             426..683
                     /locus_tag="ACHURS_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFR1239923.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(733..1683)
                     /gene="uspE"
                     /locus_tag="ACHURS_004358"
     CDS             complement(733..1683)
                     /gene="uspE"
                     /locus_tag="ACHURS_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFR1239924.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1835..2587)
                     /gene="fnr"
                     /locus_tag="ACHURS_004359"
     CDS             complement(1835..2587)
                     /gene="fnr"
                     /locus_tag="ACHURS_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFR1239925.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2782..3297)
                     /gene="ogt"
                     /locus_tag="ACHURS_004360"
     CDS             complement(2782..3297)
                     /gene="ogt"
                     /locus_tag="ACHURS_004360"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFR1239926.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3308..>3734)
                     /locus_tag="ACHURS_004361"
     CDS             complement(3308..>3734)
                     /locus_tag="ACHURS_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HFR1239927.1"
                     /translation="FIAVGLTDILESSGLSGIPAFVGLALLSSFLCMFIASGSAIWSI
                     LAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPFVPLFLGFLQRYKPDAKLGT
                     YYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS"
BASE COUNT         1003 a          886 c          893 g          952 t
ORIGIN      
        1 ataatgctac caacagcact gataattatg gtgtagatta aaaattgcac cgcatggaat
       61 aaaaaacctt ttcttttacg atttccgcgg cgaaaccaaa agctcatcag aatcaatgct
      121 gcgcagctac cgaaaataat gaccagatta agcgcattat ttgtaaacag ttcagcgatc
      181 attgttttat caggctcctc cagataattg tcgtcatgcc ggaaacccct ggcggggcta
      241 ttttaccgcg acaattcatt cagatcatca atagtcaggg aaggaagtag caacattagc
      301 taactaatca atgcgttgat tgtaaatcca gctaagaggt gaggttttca gagcagacaa
      361 cggtgaaatg tcatggtatt gttacgttta ggtaacaaga aatttgtctg cacaaggatt
      421 acatcatgat tatggcgaaa ctgaagtcag cgaaagggaa aaaatttctc tttggtttgt
      481 tggcggtttt cattattgcg gcgtcggttg tgactcgcgc gactatcggc ggcgttatag
      541 aacagtacaa tattccgctg tctgagtgga cgacatcaat gtatgtgatt cagtcatcga
      601 tgatttttgt ttatagcctg gtctttactg tgttgctggc aatcccgttg ggaatttatt
      661 tccttggcgg cgaagagcag taagtaaaaa ataggcccga taactcgggc cttgtcagtt
      721 attgaagagt cgttaatcgt cttcttcgtc atccagttca acgggtgtct gatactggtc
      781 aggtttaatg accaacagat cgcagcgaag atgatcaatc acctgttccg ccgtgttgcc
      841 gaggaatgct gctgaaatac cggtgcgtcc taccgtgccc agaaccacaa tccccgcctg
      901 taagtgctcc gccaaatcag gaataacctc ttccggcaga cctttttcta cgtgcgtcat
      961 gttttcatta atgccgaatt tctgccgcag agctttcatt gccagcaaat gttgcccacg
     1021 aatggcatcg ttataaacgc tcggatcaaa ttccggcagt tcaatcgcga tattaattgg
     1081 cgttactggg taagcgccaa ccagatgaac ttcggtatgg ttgacttgtt ctgccagttc
     1141 gatcgtctct ttgaccagtt tttcattgag cgcattatga tacggctctt cactggcgag
     1201 attcaccgcc accagcgcct tacctccttc cggccacggc tggtctttca ccatccacac
     1261 cgggcttggg catttgcgta acagatgcca gtccgttggc gtaaaaatca ccgcttccag
     1321 acggtcatgt tggtgcgcca tttttagcac caaatcgtgc ccgccgctga tcacttcctg
     1381 aatgatggct tcgaagggac ggttatgcca gaccacttta atttcaatgg gaacgccagc
     1441 attgagataa tattttgcct gctcgtggat ccaggctgta cgctggctga tgacgccctg
     1501 acgcatagcg gtacgttcgt ccggggagag cagggtggtc atttcgtatg agaagtcata
     1561 gatcggcaaa aaggctttaa ttttgccacc aatccgttga tgtaaataaa cagctcgccg
     1621 caatgctggt tggtcgtcct ggttaggatc gataacaacg agcatgttct gatacatagc
     1681 catacagggt ctccttacaa caactgtcaa cgcagtttgt aattaaaaga ttaacccata
     1741 tctggtgaat gaaacagtga tgaaccttct gccagatcaa taaatcagaa aaatttaatg
     1801 atatgacaga aggatagtga gttatgcgga aaaatcaggc aacattacgc gtatgaccag
     1861 caagctgggc cagcgcatcg ttattttcga tggtgatgta tttacctttg actgccagca
     1921 taccgctttt ctggaagcga cctagcagac ggctgatggt ttctaccgtc aggcccagat
     1981 agttaccgat atcgccacga gtcatcgtca ggcggaattc acgaggggag aagccgcgtt
     2041 gggcaaaacg acgggacagg ttgtagatga atgcagccag acgttcctcg gcatttttct
     2101 tcgacaacag caagatcatg tcctgatcgc ctttgatttc accgctcatc agacgcatca
     2161 tctgctgacg cagattcggc attttaccgg acaaatcgtc cagcgtttcg aacgggattt
     2221 cacataccat cgaggtttcc agcgcctgag cgaagctcgg gtgatggccg ctgccgatgg
     2281 cgtcaaatcc caccaggtcg ccagctaaat ggaaaccagt gatttgctcg tcgccttgct
     2341 cagtgatggt ataactttta atcgtaccgg agcggatggc ataaagcgat ttaagttcat
     2401 caccagcctt aaacagcgtc tggcctttct gaataggctt cttccgctca atgatattat
     2461 caagctgatc aagctcatgt tcgttgagtg tgaacgggat gcaaagctgg ctgatgctgc
     2521 aatcctggca atggatagca caaccgccag actgaatgcg ccgtataatt cgcttttccg
     2581 ggatcatagg tctgctcaag ccgtaattga tatttgtcaa ttttaacatc tttttaggga
     2641 gcaagtaagt ctaagcaaac cttaacagca gagaattccg atattagatg taaatatatg
     2701 tctatctatt tgaaaaccct taagttgtta agggtaactt tacataaaag tgtgaacaag
     2761 ctggcacaaa ttgtttaatg tttacagcaa aagataacct tcgtggcgca ataaccactc
     2821 ttttcgctga actccgcctg catatccggt catggtgccg tttcggccaa taacccgatg
     2881 gcaaggtacg acgatgctga tgggattcga tccgtttgcc gcaccaacgg cacgcgccgc
     2941 gccagggcgg cccaattgtt cagccagttg gccgtaatgc attacctgtc cgcaggggat
     3001 agtgcgtaga gttttccaga cttcgcgctg aaatggcgtc ccccctgtgg cagtgggaag
     3061 cgtatcaata atgctaagat taccggcaaa ataatcacga agcttgtcgc ttaaaccgcc
     3121 tgggttggtg gcagaaatgc gctcatagcc ttctttgcga taatggatgt ccagcagctg
     3181 caccatgcgt tcgctgtact cttcccattc aaccgcccgc aggcgaaatt gctcatcgca
     3241 aatcacccac agtggaccca gtggcgtggc aattttttct tcaagtaatc tcagcatccg
     3301 ttctctctta agacaaacgt gggtaaatcc ccggacctat cggcagacca acaagatacc
     3361 acgccagcaa catcagcagc cataccacca aaaagataag cggatagggc aagactaacg
     3421 aatagtaagt acccagtttc gcgtctggtt tgtagcgttg caggaatcca agaaacagtg
     3481 gaacaaaagg agataccggc gctaaaggca atacggatga gtcggcaata cgaaagagga
     3541 tttgcgcaaa tgccgggtga aagccaagta gcataaacat tggtacgaaa atgggggcca
     3601 gaatcgacca gattgcggaa ccgctggcaa taaacatgca taagaaagag gaaagcaacg
     3661 ccagaccgac aaacgccggg atgccgctaa gccctgaact ttccagtata tcggtcagcc
     3721 ccacggcgat gaat
//
LOCUS       DAZPDU010000285         3627 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZPDU010000285 DAZPDU010000000
VERSION     DAZPDU010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.289"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(89..652)
                     /locus_tag="ACHURS_004362"
     CDS             complement(89..652)
                     /locus_tag="ACHURS_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFR1239928.1"
                     /translation="MNRTSIEKLPSLTHNHLPVMTTELLADLYGTERQRLTNNFNRNK
                     ERFIEGKHFFLIEGDALRELKNENSLRVSVKIARNVRSLILWTERGAARHAKMLETDQ
                     AWEVFEKLEDCYFNQKQPPAAQNTSIENDGCALLSHFDKHGQVEFTEKVPADAMVCTL
                     ERFKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(756..974)
                     /locus_tag="ACHURS_004363"
     CDS             complement(756..974)
                     /locus_tag="ACHURS_004363"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239929.1"
                     /translation="MGELKSSPVGFPVVPTLCVSPPDDWNLTVLTVSGLQFTFPRGAQ
                     NTSKAVSTPDSHVVFLCPEFLVYDRVCG"
     gene            complement(1133..1519)
                     /locus_tag="ACHURS_004364"
     CDS             complement(1133..1519)
                     /locus_tag="ACHURS_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFR1239930.1"
                     /translation="MARDIQQVMERWGAWAANTHEDVSWASIAAGFKGLIPSKVKSRP
                     QCSDDDAMIICGCMARLNKKNQDLHDLLVDYYVGGMTFMSLARKHGCSDTCIGKRLQK
                     AEGVIDGMLMMLDIRLEMDRYVERIM"
     gene            complement(1509..1880)
                     /locus_tag="ACHURS_004365"
     CDS             complement(1509..1880)
                     /locus_tag="ACHURS_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR1239931.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKVGERYRRDVTLIVRQQ
                     RLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVRGQ
                     LVPGGRLGVKIYEITGDNDGA"
     gene            complement(1880..2137)
                     /locus_tag="ACHURS_004366"
     CDS             complement(1880..2137)
                     /locus_tag="ACHURS_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239932.1"
                     /translation="MNNQTMTFTSEQLRKHAQEMLRQAEQLEKTGVTKDAIRRDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETKVGKFEKMVQEVLR"
     gene            complement(2134..3525)
                     /locus_tag="ACHURS_004367"
     CDS             complement(2134..3525)
                     /locus_tag="ACHURS_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR1239933.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADHEVMDILITLPADIFSVRA
                     YRDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWARFSQGVGRMTG
                     RNIWMVDQANLTIDEICATTKHHLIKHPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQATGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESAQVLSTPMRQPQPRRYS
                     NTRTDSSKMERFF"
     gene            complement(3522..>3627)
                     /locus_tag="ACHURS_004368"
     CDS             complement(3522..>3627)
                     /locus_tag="ACHURS_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR1239934.1"
                     /translation="FISPRIADRITDGGRNWLSFNWPSYRSCIRGVAA"
BASE COUNT          850 a         1013 c          811 g          953 t
ORIGIN      
        1 catcggcggg tttaagctgt gtggcgtagt aaccactctt aacaggatat tcaacttttt
       61 acgatcgtaa agttttcgga aaaaattttt aaaaccgcat caaccgctcc accagttgct
      121 ctttacgggc aacgatccac ccgtgttgct ccagataaaa tttaaaccgt tccagagtac
      181 ataccatcgc atcggcgggt accttttccg tgaactcaac ctgaccgtgt ttatcgaagt
      241 ggctcagtaa tgcgcatcca tcattttcga tagatgtgtt ttgtgccgct ggtggctgtt
      301 tctggttgaa ataacagtct tccagttttt cgaacacttc ccacgcctga tcggtttcga
      361 gcattttggc gtgacgggct gcgccacgtt ctgtccagag gatgagggag cgaacgttac
      421 gggcaatttt cacagaaact cttaaagagt tttcgttctt caactctcgt aacgcatcac
      481 cttctatcag gaaaaaatgt ttaccttcta taaaccgttc tttgtttcga ttgaaattgt
      541 ttgtcaaacg ctggcgctct gttccgtaca aatcagccag cagctctgtt gtcatgacag
      601 gaagatggtt atgcgtaagt gacgggagtt tttcaataga cgtgcgattc atagacatgt
      661 cctttcgaat attttgattt accccttttg agagggtgac cgggcgctca aaaccgtcga
      721 aagtcggcgg gcgtatttcc attgctggta ttgtattagc cgcacacccg gtcataaacc
      781 aagaattctg gacacaaaaa aaccacatgg ctatcgggtg tggataccgc tttcgaggtg
      841 ttttgagcac cgcggggaaa tgtaaactgc aaacctgaaa ctgtcaacac cgtcaggttc
      901 caatcatcgg gtggtgagac gcacagggtt ggaactaccg ggaaaccgac cggcgagctt
      961 ttcagctccc ccatgcgccc caccataatt cagatgtgcg cgtgcatacg acaataaaaa
     1021 acacgctcgc ggcgtgtgtc tgtcgcggtc tctatccggg gttccaatcc cgacgccaga
     1081 ttttgctggc gcgtgaggaa tatagccccg gacaatgtgt ctggtcaagc acctacataa
     1141 ttcgttctac gtatctgtcc atctccagcc ggatatcaag catcatcaac atgccatcaa
     1201 taaccccttc cgctttctgc aggcgcttgc caatacaggt atccgaacac ccatgtttcc
     1261 gtgccagact cataaaagtc attccaccta cgtaataatc caccagcaaa tcgtgcaaat
     1321 cctgattttt cttgttcaac cgggccatac agccacaaat tatcattgca tcatcatcag
     1381 aacactgagg gcgtgatttc actttcgacg gaattaaccc tttaaaacca gcagcgatcg
     1441 acgcccagga tacatcttca tgagtgtttg cagcccaagc cccccaccgt tccataacct
     1501 gctgaatatc acgcgccatc gttatcacct gtgatttcat aaatcttcac gcccagtcgc
     1561 ccaccaggaa cgagctgacc gcgcacaata ttgatttcat caaactgctc gtcgtctatg
     1621 agtagtccgg catgcgtcag cgcatccagt ggtgctttca ggatattgtc caggtcacga
     1681 cggcgcttat ccggtggctc tgcaataatc tttatcgcca gccttccgga caggtttaat
     1741 ttcagccgct gctggcgaac aattagcgtc acatcacggc gataacgctc accaactttt
     1801 gatacaaaat atgtgctgcc acgacgtcgc cagtaagtat tcaccgtcgg cggataaggt
     1861 aaaataaact catggcgcat cagcgcagca cctcctgcac cattttttca aactttccaa
     1921 ctttggtttc cagctctgcc acacaatcaa cgagctcatc caccgctttt tgtgcgcgat
     1981 gtttcgcctg catcagttcc ctgagcgctg gcaccatatc ccgacgaatg gcatcttttg
     2041 ttacacctgt tttttccagt tgttccgcct gtcgcaacat ttcctgcgcg tgtttacgca
     2101 attgttcaga ggtaaaagtc attgtctggt tgttcaaaag aaacgctcca tcttactgct
     2161 gtcggtacgt gtattgctgt atctgcgcgg ctggggctgc cgcattgggg tggaaagaac
     2221 ctgtgcgctt tcctggtcca caggcagaaa atgtccgtta taaaaacgcc ggtaaatcgt
     2281 ccccagagaa ccgttacgtt gtttcgtgat attaatttct gctatgcctg tagcctgtgt
     2341 atccgggttg tacacttcat ccctgtaaag catcagaatg atgtctgcat ccgcctctat
     2401 ttctccggaa tttttcaggt ctgagttcat gggacgttta ttgggtctgg actccacacc
     2461 gcgggagagc tggctcagcg caatcaacgg aaaaccaccg gattttgcca ggcctttaag
     2521 cccctttgag atttcaccca cggcaaggtc atgacgcccc gtggttcggg tttttatcag
     2581 cccgagataa tcaaccacca ccagcgccgt ttccggatgt ttaatcagat ggtgtttcgt
     2641 tgttgcgcat atctcatcaa tggtcaggtt cgcctggtcc accatccaga tattgcgccc
     2701 ggtcatccgc cccacccctt gtgagaaacg cgcccagtct tcatcttcaa agtgagccac
     2761 agatttcagg cgtgatactg gcatccctcc agccgcagac accatacgtt caccaatctg
     2821 gatgttcgcc atctccatgg tgaacagaag cacaccatgc ccctgctcag tcaccttgtc
     2881 gatgatgtcc agcgcaagtt cggttttccc catcgaagga cgggcggcaa tgaataccag
     2941 gtctccgggc tccataccgc ctgtttttgc gtccagttca tcaataccgg tcatcaacgt
     3001 cctggatttc tccagcccct gattccggca ttcaacacgc tcaaccactt ccggaagcac
     3061 atcatcaata tgtaccggct gaatgacgcc ctttcctgtc gacagtgtga ccatcatgtt
     3121 ctgcgcatcc ttcagggcat cttcagctgc ttcacaggta tgcgcatcac gtaatttctg
     3181 cagcgcctca ttcagtgttt tttctgcatc gcgcagtgcg gcattgcgcc gcaacgctgc
     3241 aacatagtgc tccagtgaag acttcaccca ggttttacgc ccggtatcag taatcaccgg
     3301 ggcaagttcc ggcatctcat tacacaacag cacggggtca atcactcctg aaacacgggc
     3361 ctgtctgcag atgcctgtgt agatatcccg atacgctcgt acagaaaaaa tgtccgccgg
     3421 tagtgtgatc agaatatcca tcacttcatg atctgcccca cgcagaaaga atgcgccaat
     3481 gacagcgcct tccaggtcat cattacgcca gactggtgtt gtcatgcagc cacacctctg
     3541 atacaagaac ggtagctggg ccagttgaac gacaaccagt tgcgcccccc gtctgtgatc
     3601 ctgtcggcaa tgcgggggct gatgaac
//
LOCUS       DAZPDU010000286         3596 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZPDU010000286 DAZPDU010000000
VERSION     DAZPDU010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3596
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.290"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(87..1484)
                     /gene="ccp"
                     /locus_tag="ACHURS_004369"
     CDS             complement(87..1484)
                     /gene="ccp"
                     /locus_tag="ACHURS_004369"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFR1239935.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLGVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1889..3538
                     /locus_tag="ACHURS_004370"
     CDS             1889..3538
                     /locus_tag="ACHURS_004370"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFR1239936.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT          917 a          929 c          853 g          897 t
ORIGIN      
        1 aaacatttaa catgataata ttaaaaacag actttattat cgtaataaaa atcgcccaaa
       61 cattcctgtt cgggcgatca aattaattat tgtttatcct gcatatacgg cgtgtacacc
      121 ccgttcagac tgtgcaggaa agctacgata tcatccacat cctcctgcgg cagctctttg
      181 cctacctgat agcgcagcat cagtttcacc gccccgtcca gcgtcggcac gtcaccgcga
      241 tggaagtacg gtgccgttaa agcaacgtta cgtaaaccgg gtactttctg acgcaattta
      301 tcacgctctt ctttagtaac attcatacga ccaatatccg ccgccgtaat ttccccaaag
      361 ttaaagtctt ttttcagccc caacggttca aaggaacgtc caccgagaat aataccacca
      421 tgacaagttg cacatttatt atctttaaat aattgatagc cttttttctg ttgcgccgtc
      481 agcgcatttt catctccacg caaccattta tcaaatgggg aatccggcgt aattaatgtt
      541 ttctcaaatt cagcaatggc atcagtaata ttttcgccac tgaaaccttg cggatagacg
      601 ccgaggaact gcgctttaag ctgcggatct ttttccagct tagcaataat ttcgtcccaa
      661 gatttcgacg ccatttcaat cgggttcaac ggcggtccac cagcctgatc ctgcaatgtt
      721 gccgcacgac catcccagaa ctgctcaacg ttaaatactg agttaaatac cgtcggcgcg
      781 ttaatcggcc caactgcgcc accaacacca atcgatgttt ttctgccatc gacgcccccc
      841 gcattcaacg catggcaatg agcgcatgaa atggtgctat cagccgataa acggggatcg
      901 tgatacagcg caaagcccaa cgccactttt tgcgcatcgg taggcagttt ttgcgggatg
      961 ggctgcaccg gttcattgcg atgctccgga gcagtatcat tgctggcgta atattccgcg
     1021 cgctgttttg caatccaggc cagtatttcc gcccgctctt catcactcac cttacccgcc
     1081 cagtgaagcg cggtataacg cgttggcggc atagtttcat actgcatcac ccattcaatc
     1141 ttattcagat cgctttgcga aacgggttta tcagccagca gtgccgcacg cacggcctcg
     1201 aggttaaaag atttatatcc aagcttaatg tcgtaatcca tcaactgttt cgcgccagga
     1261 atataataat aggcgggtaa ttctgccgaa ggcgtgtggc aatagtcgca tcctttttcg
     1321 cggagaaaac ctaaaacctt attattttca ctgacagcgg atgcctgaac atcggcctgt
     1381 ttactgcgtt tattatcgtg gtaccacaca taaccagata accctaaata gcaaatcgcg
     1441 acgccagcca ggccgatcgc ggtaatacgt gagaccattt tcattattct tccctcacgg
     1501 tggtgagtta ttgtgaccca gaaaattttc cagaatgctg caaacgtctc tgcgagacaa
     1561 agccatcata aaaaacagtg agatacgctt tttgatagga atcaatcaat tacatgaata
     1621 taaactatca ataagatagc ctgtgcctat tacccagatc aggaatggca tatcatgatt
     1681 gcggcgagag caggattgaa tgttctgaaa atgaaaaaca acacgaaatc cgtattttta
     1741 taaattccat ggatattagt gcgttatatt ttttgacagg ttaataaacg tgatccaccg
     1801 cacgctttgt cgcccaccag gcggagcgaa tgactaccct taaagaaaag cccgataatt
     1861 agcgacgaat ttcggaggtt ggatccttat gctcaatcag aaaattcaaa accctaatcc
     1921 agacgaactg atgatcgaag tcgatctctg ctatgagctg gacccgtatg aattaaaact
     1981 ggatgagatg atcgaggcag aaccggaacc cgagatgatt gaagggctgc ccgcctctga
     2041 tgcgctgacg cctgccgatc gctatctcga actgttcgag catgttcagt cggcgaaaat
     2101 tttcccggac agtaaaacct ttcccgactg cgcacctaaa atggacccgc tggatatttt
     2161 aatccgctac cgtaaagtgc gccgtcatcg tgattttgac ttgcgcaagt ttgttgaaaa
     2221 tcacttctgg ctgccggagg tctactccag cgagtatgta tcggacccgc aaaattccct
     2281 gaaagagcat atcgaccagc tgtggccggt gctaacccgc gaaccacagg atcacattcc
     2341 gtggtcttct ctactggcgc tgccgcagtc atatattgtc ccaggcggcc gttttagcga
     2401 aacctactat tgggactcct atttcaccat gctggggctg gcggaaagtg gtcgggaaga
     2461 tttgctgaaa tgcatggccg ataacttcgc ctggatgatc gaaaactatg gtcacatccc
     2521 caacggcaac cgcacctatt atttgagccg atcgcaacca ccggtttttg cgctgatggt
     2581 ggagttgttt gaagaagatg gtgtacgcgg tgcgcgccgc tatctcgacc accttaaaat
     2641 ggaatatgcc ttctggatgg acggtgcaga atcgttgatc cctaatcagg cctatcgcca
     2701 tgttgtgcgg atgccggacg gatcgctgct caaccgttac tgggacgatc gcgacacgcc
     2761 gcgtgacgaa tcctggcttg aggacgttga aaccgcgaaa cattctggtc gcccgcccaa
     2821 cgaggtgtac cgcgatttac gcgcgggggc ggcctccggt tgggattact cttcccgttg
     2881 gctgcgtgat actggtcgtc tggcgagcat tcgtaccacc cagttcatcc ccatcgatct
     2941 gaatgccttc ctgtttaaac tggagagcgc catcgccaac atctcggcgc tgaaaggcga
     3001 gaaagagaca gaagcgctgt tccgccagaa ggccagtgcc cgtcgcgatg cggtaaaccg
     3061 ttacctctgg gatgatgaaa acggcatcta ccgcgattac gactggcgac gcgaacaact
     3121 ggcgctgttt tccgctgccg ccattgtgcc gctctatgtc ggcatggcga accatgaaca
     3181 ggccgatcgt ctggcaaacg ccgtacgcag ccggttactg acacctggcg ggattctggc
     3241 aagcgagtac gaaaccggtg aacagtggga taaacccaac ggctgggcac cgttacaatg
     3301 gatggcaatt cagggattta aaatgtatgg cgatgacctt ctgggtgatg aaatcgcgcg
     3361 cagctggctg aaaacggtga atcagttcta tctggaacag cacaaaatga tcgagaaata
     3421 ccatattgcc gatggtgttc cccgcgaagg cggcggtggc gagtatccgt tgcaggatgg
     3481 gtttggctgg actaacggtg tggtacgccg tttaattggt ttgtacggcg aaccataata
     3541 tttttacagc cagccgctaa cttcctgctg gctgtaaaat tatcctcttc aggagg
//
LOCUS       DAZPDU010000287         3564 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZPDU010000287 DAZPDU010000000
VERSION     DAZPDU010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.291"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            160..345
                     /locus_tag="ACHURS_004371"
     CDS             160..345
                     /locus_tag="ACHURS_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015690166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239937.1"
                     /translation="MKKVTLKERKHHVVTARLNDDQVLVLNQLISSGKVQKDSLSACV
                     QYLINQYMILQGGGNEE"
     gene            335..637
                     /locus_tag="ACHURS_004372"
     CDS             335..637
                     /locus_tag="ACHURS_004372"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239938.1"
                     /translation="MKNDFDLVQYFDDYTIVGDMDYHYFYCVKYQEGSLEAHYERIGD
                     PEVLGTITWNGNYLIDGLPMVMFVLAAEDEHKECLNRLLIVCEKARDEYRLNATGL"
     gene            692..1246
                     /locus_tag="ACHURS_004373"
     CDS             692..1246
                     /locus_tag="ACHURS_004373"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR1239939.1"
                     /translation="MNPKITWRLIPNSKHYYVSENGDVYNDKTKQYIATKNPSNGYKK
                     VSINGKSLYVHVLVCTLFNGAKPIGGRYTVDHIDRDKMNNKAKNLRWVTQKVNNSNRK
                     HKGTRTQKLTLKQWLMIVGEYLKGQLSQYAITKWFLNQTGINICPQNIGKLFKGTTYS
                     KWYTTYLTNKQRQRIQSLTIQRKP"
     gene            1368..1829
                     /locus_tag="ACHURS_004374"
     CDS             1368..1829
                     /locus_tag="ACHURS_004374"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239940.1"
                     /translation="MAKTYSTFDNRNIKALISQYKLYKDIPCEDIKNIMKDSVAKLKV
                     RDKISVYDVFAVLSNEDELRNDLLFNTINAIKVQQNKKQVKERMIRYYGAAITDAAKR
                     IEQYMLAHPNALPAPVYGQRTLTYKEFGKLLELQKQGDFIDDMIEYLKGLR"
     gene            2293..3036
                     /locus_tag="ACHURS_004375"
     CDS             2293..3036
                     /locus_tag="ACHURS_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFR1239941.1"
                     /translation="MNRKQFIQSHGATCSNWTWSWSFVNHDKKMVIFGAWDVQKEQER
                     SVILRERWKTKVVEGKERKNLGYIQAIEHIQLIAEGYELYTFDMEQGRNDENRDVAVI
                     KRFTPKLEKRYLRKEGTVWYADFLPNPFPDEITSPENYAEGAKKQVTVNSYERDPKAR
                     QACIDHHGTSCGCCEFNFEKVYGEHAKGFIHVHHIKPLHTVGEDYEVNPITDMVPLCP
                     NCHAMVHRGNEVLSVESLKALLFKDSNKI"
     gene            3119..>3564
                     /locus_tag="ACHURS_004376"
     CDS             3119..>3564
                     /locus_tag="ACHURS_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239942.1"
                     /translation="MSITTIAATYKDVLTIAFSALSFIIACTSLYFSGRAAWHDRSRL
                     KITADLVYESFDEEPYKIEVIVINVGRRDAVLEGMLCHYEKGIRSHSSPKESIVIKEK
                     QRKKFELVSSDLVTIDNECDICHLEDITILDVEGNEHKIPNSHRLIA"
BASE COUNT         1263 a          596 c          707 g          998 t
ORIGIN      
        1 aacgataatg cgttttgcct tccgctccag ctttgaacat aaataatata gcgacaataa
       61 atttcctttt taattaagat tgcctctctt gcgagagagg tatcttttta atttggattt
      121 ataaatacag tatacaaata cacctataag gatatagaaa tgaaaaaagt aactttaaag
      181 gaacgcaaac atcatgtagt aaccgcacgt cttaatgacg atcaagtact tgtacttaac
      241 cagctaatta gtagtgggaa ggtacagaaa gattcattat cagcctgtgt tcagtactta
      301 attaatcagt acatgatttt acagggcggt ggtaatgaag aatgactttg atttagtaca
      361 atattttgat gattacacaa tagtagggga tatggattat cactatttct attgtgtgaa
      421 gtatcaggaa ggttcattag aagcacatta tgaacgaatt ggtgatccag aagtattagg
      481 tactattaca tggaatggaa attacttaat cgacggatta ccaatggtaa tgtttgtact
      541 ggctgctgaa gatgaacata aggaatgttt aaaccgtttg ctaatcgtat gtgagaaggc
      601 gagggatgaa tataggctaa atgctactgg cctctaagta gtagcagacg cgatcacatg
      661 gagtgacgcg ataacaacaa ggacgtttga tatgaaccct aaaatcactt ggagattaat
      721 tccaaattca aaacattact acgtatctga aaatggcgat gtttacaatg ataaaacaaa
      781 acagtatata gctacgaaaa acccatctaa tggatacaaa aaagtttcta ttaatggcaa
      841 atcactatat gtacatgttt tggtttgtac tttattcaat ggtgctaaac ccattggagg
      901 aagatacact gtagatcata tcgaccgcga taaaatgaat aacaaagcaa aaaatttacg
      961 ttgggtaact cagaaggtta ataattccaa tagaaagcat aaaggtactc gtactcaaaa
     1021 gcttactcta aagcaatggt taatgattgt tggagagtac cttaaaggac aattatctca
     1081 atatgcaatc actaaatggt ttttaaatca aactggtatt aatatttgcc ctcagaatat
     1141 aggtaagtta ttcaaaggta ctacctattc aaaatggtac acaacatatc ttacgaataa
     1201 gcaaagacaa agaatacaaa gcctaaccat tcaacgtaaa ccataaaaaa tgccagtatt
     1261 ccagtactgg catctattca aaactcaatg gcaatgaagc caaacttatt aaatggcctg
     1321 taatcactac gggtcacgat taattaattt atacaggatt atttatcatg gcaaaaacct
     1381 attcaacttt tgataacaga aacataaaag cactaattag ccagtacaag ctatataaag
     1441 atatcccatg cgaagacatc aagaacatca tgaaggactc agtagctaaa cttaaagtga
     1501 gagataagat tagtgtatat gatgtgtttg cagtattgag taatgaagat gaattacgga
     1561 atgatttact attcaatact atcaatgcta ttaaagtaca acagaataag aaacaagtaa
     1621 aagaacgtat gatcaggtac tatggggcag cgatcacgga tgcagcaaaa cgaatagagc
     1681 agtatatgtt ggcacaccca aacgcactgc cagcaccagt atatggacaa agaacactaa
     1741 catataaaga gtttggtaaa ctattggaac tacaaaaaca aggtgacttt attgatgaca
     1801 tgatcgagta cttaaagggc ttgcgttaat tgtaacttat tacggcatat tacggcatgg
     1861 atgtatgccg taatacggca aaaaaagccc ctgaaacccg ctatttacca tcaatacggc
     1921 aactaagtat agagatacga aatccattcc atttcattcc atgtatttct ttctctatca
     1981 gtattccaag aaactgtgac ttgcgtcacg aatactactc gtcgtgaact cctcgtagta
     2041 ttggttagta acgttgcgtt tttctcgcca gtactcgaaa acccgcaagg cgttactact
     2101 cgctttgctc gatagatgtt agtacagcaa atgaagtacc taaagtgaat tggtaagtac
     2161 gacatgcccc gccagttcca caactctcgc tctggtcatt tccactacta tgatgctatt
     2221 ggatagctac ataaagtact ggatcaagta ggcatttttt gtgtacaaag agggaattaa
     2281 gagagggata ccatgaacag aaaacaattc atccagtcgc atggtgctac ttgtagtaac
     2341 tggacttgga gttggtcatt tgtgaaccac gacaagaaaa tggttatctt tggtgcatgg
     2401 gatgtacaaa aagagcaaga aagatcagtc attctgaggg agcggtggaa aaccaaagtt
     2461 gtggagggaa aggaaagaaa aaatcttgga tatattcaag ctattgaaca cattcaattg
     2521 atagctgaag gttatgagct ttacacgttt gatatggagc agggacgaaa cgatgagaat
     2581 agagatgtag ctgttatcaa acggttcact ccaaaacttg aaaaacgtta ccttcggaaa
     2641 gaaggaacag tttggtatgc agacttttta cctaatccat ttccagatga aattacctca
     2701 cctgaaaact acgctgaggg tgcaaaaaag caagttacag taaactccta cgagcgtgat
     2761 ccaaaagcac gacaggcttg tattgaccat catggtactt cctgcgggtg ttgtgagttt
     2821 aactttgaga aagtctatgg tgaacacgcc aagggcttta tacacgttca tcatatcaaa
     2881 ccgctacaca cagtaggcga ggattacgag gtaaatccaa ttactgatat ggttccactt
     2941 tgtccaaact gtcacgcaat ggtacatcgc ggtaacgaag tattaagtgt cgaaagttta
     3001 aaagcactgt tgtttaaaga cagcaataaa atttgaattt attttagact tgatacgaca
     3061 tacttgaaaa gttatgaata caatatgctt taaattagac atgtaagttg agagaaaaat
     3121 gagcataaca acaatagcag ctacatataa agatgtgtta actattgctt tttcggcgtt
     3181 gtcatttatt attgcatgta cttccttata cttctccggt agggcagcat ggcatgatag
     3241 atcaaggcta aaaataacag ctgacctcgt ctacgagtct tttgatgagg aaccttataa
     3301 aatcgaggtg atagtgatca atgttggtag gcgtgatgca gttttggaag ggatgctttg
     3361 ccattatgaa aaaggcatta ggagccattc atctccaaaa gagagcattg ttattaaaga
     3421 aaagcaaaga aaaaaatttg aattggtaag ctcagactta gtcacgatag ataatgaatg
     3481 tgatatttgt catttagaag atataactat tttagatgtg gaggggaatg aacataaaat
     3541 ccctaactct cacagactca ttgc
//
LOCUS       DAZPDU010000288         3560 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZPDU010000288 DAZPDU010000000
VERSION     DAZPDU010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.292"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            129..1376
                     /locus_tag="ACHURS_004377"
     CDS             129..1376
                     /locus_tag="ACHURS_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HFR1239943.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(1905..2105)
                     /gene="torI"
                     /locus_tag="ACHURS_004378"
     CDS             complement(1905..2105)
                     /gene="torI"
                     /locus_tag="ACHURS_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator inhibitor TorI"
                     /protein_id="HFR1239944.1"
                     /translation="MQHELQPDSLVDLKFIMADTGFGKTFIYDRIKSGDLPKAKVIHG
                     RARWLYRDHCEFKNKLLSRANG"
     gene            complement(2228..2572)
                     /locus_tag="ACHURS_004379"
     CDS             complement(2228..2572)
                     /locus_tag="ACHURS_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239945.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGAPH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKTYGNKAP
                     ETYRRISELRKD"
     gene            2814..3416
                     /locus_tag="ACHURS_004380"
     CDS             2814..3416
                     /locus_tag="ACHURS_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239946.1"
                     /translation="MSYFLCKKWMINLSGAGKILWALNMKKDSYPYLICMTVSGLIFI
                     FLFFWWRADIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQSGWKSLSAYLK
                     VYAGICIFSGFFLIIPLTTLTYFLPGETSSYVAPYRYTSGSSKSCSGAEVDDPDLHEN
                     IRICYPYGNYEYDNIIYVEKKINTLGAVVTYAQTARDDTE"
BASE COUNT          887 a          715 c          816 g         1142 t
ORIGIN      
        1 gcagtaacgg cgaaaggggc aatgacagcg ctgccatgtc gacaagaact ggaatagtga
       61 gaagtaaacg gcgaagtcgc tcttatctct aaataggacg tgaatttttt aacgacaggt
      121 aggtaattat ggcactgaat attccattca gaaatgcgta ctatcgtttt gcatccagtt
      181 actcatttct cttttttatt tcctggtcgc tgtggtggtc gttatacgct atttggctga
      241 aaggacatct aggattgaca gggacggaat taggtacact ttattcggtc aaccagttta
      301 ccagcattct atttatgatg ttctacggca tcgttcagga taaactcggt ctgaagaaac
      361 cgctcatctg gtgtatgagt ttcatcctgg tcttgaccgg accgtttatg atttacgttt
      421 atgaaccgtt actgcaaagc aatttttctg taggtctaat tctgggagcg ctcttttttg
      481 gcctggggta tctggcggga tgcggtttgc ttgacagctt caccgaaaaa atggcgcgaa
      541 attttcattt cgaatatgga acagcgcgcg cctggggatc ttttggctat gctattggcg
      601 cgttctttgc cggcatattt tttagtatca gtccccatat caacttctgg ttggtctcgc
      661 tatttggcgc tgtatttatg atgatcaaca tgtgttttaa agataaggat caccagtgcg
      721 tagcggcgga tgcgggaggg gtaaaaaaag aggattttat cgcagttttc aaggatcgaa
      781 acttctgggt tttcgtcata tttattgtgg ggacgtggtc tttctataac atttttgatc
      841 aacaactttt tcctgtcttt tatgcaggtt tattcgaatc acacgatgta ggaacgcgcc
      901 tgtatggtta tctcaactca ttccaggtgg tactcgaagc gctatgcatg gcgattattc
      961 ctttctttgt gaatcgggta gggccaaaaa atgcattact tatcggtgtt gtgattatgg
     1021 cgttgcgtat cctttcctgc gcgctgttcg ttaacccctg gattatttca ttagtgaagc
     1081 tgttacatgc cattgaggtt ccactttgtg tcatatccgt cttcaaatac agcgtggcaa
     1141 attttgataa gcgcctgtcg tcgacgatct ttctgattgg ttttcaaatt gccagttcgc
     1201 ttgggattgt gctgctttca acgccgactg ggatactctt tgaccacgca ggctaccaga
     1261 cagttttctt cgcaatttcg ggtattgtct gcctaatgtt gctatttggc attttcttcc
     1321 tgagtaaaaa acgcgagcaa atagttatgg aaacgcctgt accttcagca atatagacgt
     1381 aaacttttcc ggttgttgtc gatatctccc tatccctcaa ccggaaaata ataatactaa
     1441 agtgcttagc ccggctaata atcacctaat ccaaacgcct cattcatgtt ctggtacagt
     1501 cgctcaaatg tacttcagat gcgcggttcg ctgatttcca ggacattgtc gtcattcagc
     1561 gacctgtccc gtgtatcacg gtcctgcgaa ttcatcaagg aatgcattgc ggagtgaagt
     1621 atcgagtcac gccatatttc gctatcagga ttctgtgtga tggttacatc gcccggccca
     1681 gggctgttta gtcatcagcg ctttctgaca gtgctgagat ttcaacctgt tgcagtaaaa
     1741 atgagtagat ataaggcaag tgtgctgcca aacccatctt ttacggggtg aaggtagatt
     1801 tcgtttgaag ggtatctggt gtcccctgca gacatctact tgaggcggca ggggattgat
     1861 tggaatgctg ttttttagat ttgaaaaata ttttacccgc tattttaccc attggcgcgg
     1921 cttaagagct tatttttaaa ttcacaatgg tcacgatata accatcttgc tcgtccgtgg
     1981 ataactttgg ctttaggcag gtcgccggac ttaatccggt catagatgaa ggtcttaccg
     2041 aagccagtat cagccatgat gaatttcaaa tcaaccagtg aatcaggttg tagttcgtgt
     2101 tgcatgagtg ctatctccga atatggaatc gaacctgcaa atcaggcaat aaaaagccgc
     2161 attgatgcag caatggtagg tctggatatc ttgagaaatg aacaggcctc atcgagtgtg
     2221 aggcgggtta gtccttgcgt agctcgctga ttcttctgta agtctctggt gctttgttcc
     2281 cgtacgtctt catttcagac ttcaacagag caacgagtga atcccattcg ttgaggattc
     2341 ctttgaatgc cggaacgcgc tttgcaacct tgtcgaatga atctctgatt tctggaatct
     2401 gctcaacaag tgcaacgcat cgccgaaagt ctgctgcgtc atggggagcg ccgaagtgat
     2461 gaccatagat attctttttc aggccacatg cgattgaggc aagagttgcg ctactgatgc
     2521 cgacatcgcc agttgattgc catttcaaaa ccttcatagc caaatctgac atttcttgtc
     2581 tccataaaac aaaactcgcc gtagcgagct cagataaaag aaatccccgt cagtgcgagg
     2641 atgctgttca ttgctgctat acactttttt gctctcaacg taagcggtag cccattctgt
     2701 tgggttggtc agttgctttt aggaaatgct ctttacccct taaacgtcgg ctgaaagagc
     2761 taaaatccat gcaaaaaatt tacgcaattt tgtgtattat tgtgcagtaa gtaatgagct
     2821 attttctgtg caaaaaatgg atgataaatt tgtccggggc aggaaaaatt ttatgggcgc
     2881 taaacatgaa aaaagattcg tatccttatt tgatttgcat gacagtttca gggctgatct
     2941 ttattttcct tttcttctgg tggcgggcag atatctacag ggtcacgttt cttaatcaga
     3001 gtatatccca ctattacatt ctgtttagca tgggaatagc ttttctgtta tctctgtttt
     3061 gggttaagaa ggggatagta aaacaaagcg gctggaagag tctgtcagca taccttaagg
     3121 tttatgcagg gatatgcata ttttctggat tttttctgat tatacccctt acaacactaa
     3181 cttatttttt gcctggagag acatcgtctt atgttgcacc gtatcggtat acttccggta
     3241 gttcaaaaag ttgttctgga gctgaggtgg atgaccccga tctacatgag aatattcgca
     3301 tttgctatcc gtatggcaat tatgagtacg ataatattat ctatgttgaa aagaaaatta
     3361 atacattagg tgcggtagtg acatatgcac agaccgcgcg tgatgatact gaatgagata
     3421 gtatatagcg ggcaagtttt agttaattta tcgaggtaat ataatttacc tcgactcgtt
     3481 tattctggta tgaatatttc gctttacgat cgatttttat ctgatgatat catgcggttt
     3541 tcatatactg acttactgtc
//
LOCUS       DAZPDU010000289         3554 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZPDU010000289 DAZPDU010000000
VERSION     DAZPDU010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.293"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(181..441)
                     /gene="yfhL"
                     /locus_tag="ACHURS_004381"
     CDS             complement(181..441)
                     /gene="yfhL"
                     /locus_tag="ACHURS_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFR1239947.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            656..1846
                     /locus_tag="ACHURS_004382"
                     /pseudo
     CDS             656..1846
                     /locus_tag="ACHURS_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(2031..3218)
                     /locus_tag="ACHURS_004383"
     CDS             complement(2031..3218)
                     /locus_tag="ACHURS_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFR1239948.1"
                     /translation="MLPRFADIFQQGNHWLNWLEKQPERAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLTEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVMPDALAAEGSSKR
                     EWNRFLDTHYRRGWNVNVSRVMDNAPHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLR
                     YNSHRTKREEYLLMSGDEFMERFSWHVADKGFRMVRYYGFLSPAKRRLLEEVVYIITE
                     TVRKTAMQITWRGMYQRLLKVDPLKCVLCGSQMRFTGLKRGYRLAEQVLMHEPLARMR
                     WCG"
     gene            complement(3218..>3554)
                     /locus_tag="ACHURS_004384"
     CDS             complement(3218..>3554)
                     /locus_tag="ACHURS_004384"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239949.1"
                     /translation="KPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFDSFDDAVECCAD
                     LNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILSGISAGGMYCPECGTVH
                     WPDGVAPPF"
BASE COUNT          907 a          947 c          827 g          873 t
ORIGIN      
        1 acttaaggtg cgtccggtga accaatcgga cgcgccttta ataactataa ataagtgtct
       61 tggcagatac tatataaatt aacttagtga atgattatgc taatgtcatc aattaaataa
      121 atataatggc gttaaggctt cccagtaata taattaatac tctacttcca gagtagaata
      181 ttaaatttta tccgcgtggt gcatcagcac aaatttatcc cacaactgtt cttctgtctc
      241 gacatgcgcc ggatctttca caatagtatt ggggatcggg cacaccttct ggcaggttgg
      301 tgtctcgtag tgccctacgc attcggtaca cttatcgctg ttaatctcgt agatatgatc
      361 tcccattgaa atcgcctcat tcgggcattc gggttcacac atatcacaat taatacagcg
      421 tttagtaatt agtaaagaca tttcaatgga ttaccgttaa atcattttaa aatcagtaag
      481 ttgtatcgag tttgtatgct ttactgtcat taacttactg tatgttgatc cagtgtattt
      541 aaccttgata aactcagtcc agcaacacaa aaccgcaaca cattgcattt tgtcccgtag
      601 aaaagacttg tatgtgtgag cttgttttct gcgcctacgc agataaggat tgagaatgcc
      661 gcgcactgta acacataatc cggatagccc caataatgac gatgttttag ccgcatctga
      721 aaaatgggac gcctgtaaac ccccctatac cagcgcacac atgaaaatct gtgttgctgc
      781 cgccaaaatc atcctcgctg cttccggcgt ggctcgccgt tccaaatacg aaaaagagaa
      841 ctatctccgt atcgatttca gcaaagccgg taaggttaca ttttacgccg agtttccaaa
      901 aaagatgggc ctcaaaggta aaaagctcgg cgagtggccg gagctcgcta tccagctggc
      961 gcgcgaaaaa gcactaggta tggctgacgg tggactgcgg gcagagtccg tacatgcagc
     1021 gctggaaatg taccgggatg acctcaaagc caaagtagcc cggcagaagc tgagcccgga
     1081 cagtttcaca acctacgggg tgcgtatcga ccggattaaa gcaacgttcg gcgagcgtga
     1141 ggtgttcagc gacgtaacat acaatcggct ggtggaagtg ctggacgagt ggatcgccac
     1201 tcgctcgaac aataacgccc tggagttgtt tgccgagctc cgtcggttct ggaagttctg
     1261 cgcacctact ctttgcaacg gccgcaatgt tgccgccagt ctgccagatg attatgtttc
     1321 ctcccgcgta cagaaaccta cccccacacg gctttttacc gatattgaat caatcgcccg
     1381 actctggctc aatgttgctg cctgcacctc tgtacaccag aagaatgctg ttcgcttcat
     1441 gatcatcact ggtgttcgtc cgattaatgt ccataacctg cgctgggact acgtttacga
     1501 ggaggctggt gaaattgttt atccggaagg ggttatcggc atgcgagggg ctatgaaaac
     1561 acaaaaggct ttccgcctgc cgataacgcc tgagatccgg cggattatcg acgagcagaa
     1621 agcctggcgt gattcagttc ctgagtgcaa cagggattat gtatttttgc agccacgtga
     1681 tccaatgcag ccattttcaa aacgatcact ggataagctg gtgaaaacat acagcccgga
     1741 cggggctgta aaaggaataa aacatgatgg gactgttaaa gggaaagacg gtgcatttaa
     1801 tacgatgtgc cgtaaattcc ttaagagcaa tgttattgcc ttgatcgata ggaattaaaa
     1861 ccccaaaaag attaaaaaaa caccacaaaa cggatgtttc ttcaacacca cttttgctca
     1921 tatgaacgga accgacgatt aaactggatg gctctgattg attcagggta tgaatggcgg
     1981 ttttttgctc cgtttccctc aaaatggacg caactttccc tctgcggctc tcagccgcac
     2041 caccgcatcc gggccagcgg ctcatgcatc aggacctgct ctgccagacg gtagccccgc
     2101 ttcagccccg taaaacgcat ctgactcccg cacagcacgc acttcagcgg gtcaaccttc
     2161 agtaacctct gatacatccc tctccaggtg atttgcatcg ccgtttttct cactgtctcc
     2221 gttatgatgt acaccacttc ttccagtaac cgccgtttcg ccggactcaa aaaaccgtag
     2281 tacctcacca tacggaaccc cttatccgcc acatgccagg agaacctttc catgaactca
     2341 tctccactca tcaacaggta ttcttcccgt tttgttcggt gactgttgta acgcagaccg
     2401 atttcatcct gaccggcata atgctccaga cgactcatcg gcaccggtgg ctttttcagg
     2461 taagagccaa agtacaccgc cacatggggg gcattatcca tcacccggga tacgttgaca
     2521 ttccagccac ggcggtaatg cgtgtccagg aagcgattcc attcccgttt actgcttcct
     2581 tctgctgcca gcgcatccgg catcaccagg tcagggtatt tccgtgacag caaccgtgtt
     2641 atccggtagc gccacatgct catcacctta cgggcgtaaa aatgaagatt tttccaggtg
     2701 tggcccgacg tcacaccacc ggcagttgtc gataaatgga tatgcggatg ccactgctgg
     2761 tcacgccccc atgtgtggat caccgtgaat atccccggct ccacatctgc ctgatggcag
     2821 atttccagta tcacatccgc tgcaatgcgg ctcatctctg tcagtaacca ccggttgtgg
     2881 aacaccaggg accagtactg gcagggaagt gtgaacacaa tatgctgcca cgggcagtcg
     2941 gggaccaggc tcagcagata ctgtatccac tgtgcgccag ccttcacccc gcagtgcggg
     3001 caggagcggc ttttacaccg gaagcagacc ttttttgtat ggcaacagtc cggtgatgaa
     3061 cagcaccact gtgtataccc catcagtgtg gtcccgcacg ccatgatttt ggtcaccgac
     3121 tcaatcacca ccggacgtac tgccctttcc ggctgcttct ccagccagtt aagccagtgg
     3181 tttccctgct gaaagatatc ggcaaaacgg ggaagcatca gaagggcggg gcgactccgt
     3241 ccggccagtg aaccgtgcca cactccgggc agtacatacc gccggcgctg ataccggaaa
     3301 gaatggtcgc aaattcccgc tccgtgcagc gggcgatttc cggataccct tcgtcatcaa
     3361 cacgtacaaa ccagaagacc agctttttgt ttcccgcatc cacaaagaac ggaatattca
     3421 ggtctgcgca gcattcaacg gcatcgtcaa aactatcaaa gcgcagaact tctgcgtctt
     3481 cttcgtcaaa aaaatcatct tcgtgaagct tcacgacata gcggggaagt ttgcttcttt
     3541 gagaggcggg ttta
//
LOCUS       DAZPDU010000290         3546 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZPDU010000290 DAZPDU010000000
VERSION     DAZPDU010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.294"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..143)
                     /locus_tag="ACHURS_004385"
     CDS             complement(<1..143)
                     /locus_tag="ACHURS_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaA/YiaB family inner membrane protein"
                     /protein_id="HFR1239950.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVL"
     gene            complement(189..530)
                     /gene="yiaB"
                     /locus_tag="ACHURS_004386"
     CDS             complement(189..530)
                     /gene="yiaB"
                     /locus_tag="ACHURS_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFR1239951.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(699..2153)
                     /gene="xylB"
                     /locus_tag="ACHURS_004387"
     CDS             complement(699..2153)
                     /gene="xylB"
                     /locus_tag="ACHURS_004387"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFR1239952.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVDSQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESVEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(2225..>3546)
                     /gene="xylA"
                     /locus_tag="ACHURS_004388"
     CDS             complement(2225..>3546)
                     /gene="xylA"
                     /locus_tag="ACHURS_004388"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFR1239953.1"
                     /translation="QAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRFA
                     ACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHDV
                     DVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDPE
                     VFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVEH
                     KHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAGH
                     SFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTGG
                     LNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNSE
                     LGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
BASE COUNT          926 a          982 c          784 g          854 t
ORIGIN      
        1 agtacggcaa aataataccc tttttcattt aactgcatct ctgcattcca tagccctaac
       61 agataggtaa cgataccacc aacgagagct atccatgaca caatactaaa ggccggtgaa
      121 taggttgata ttttgttgtc catcacagta ttcctttcat tcctgaatgt gtaagagctt
      181 tatgttgctc ataccgagcg aaacaaacgc atttgaccaa acaagcagac aaaaaaggcg
      241 atggggtaga tcaccatttc ataagcattg ataggtgcat ttaaaacacc gataaacaag
      301 agaccgatag tgattaacgc aactaactgg cagatatggg taaaaaaatt atccagttgc
      361 ccgagttttt ctgcgcgaag atatgcatag ttaccaaaag ttgctgtcat taacacgcca
      421 agaaaatagc cttttccact tagcaatggg catgagatcc atagcccaac cagataaacc
      481 agcgccccga caacaaataa tagttttgcc acagtttttg aggttttcat aacgatctcc
      541 atatctacca gcgatacatt acgagtaacc aacgaaagac aaaactgaaa aatgccatta
      601 acaaatgatt ttcagaataa attcatacta aatattaatt aattagtgag atatatagat
      661 gtgaattatc ccccacccgg tcaggcaggg gataacgttt acgccattaa tggcagaagt
      721 tgctgataga ggcgacggaa cgtttcacgt cgtggctgat aagcggcata acgctgcgca
      781 tctggtagat gcgactgttc taacggtagc tgcggcaaca actcaatgag cgatttctct
      841 ggattcgccg caatctgcgc cagccttgct gcgcccagtg ctggccccac atcccccccc
      901 gtacggtaat cgagctgctg accgctgata tccgccagca tctgacgcca gtactcacta
      961 cgcgccccgc caccaatcaa cgtaacactt tgcggtttaa tgccgcaggc atgcacgaca
     1021 tccatgccat ctgccagcgc ataacccacg ccttccagca ctgctcgcgc cagttcattg
     1081 gggccatgtt gatgagtcaa accaaagaaa acccccttcg cctggggatt attgtgtggt
     1141 gtacgctcgc cggaaagata aggcagaaac caaactggct cgacactttc atcagcctgt
     1201 tgagctgcag cgattaaagc tgggacattg cacaggccgg ttaatttcgc ggcccaatcc
     1261 agacacgatg ctgcactcag cattacagac attaaatgcc aacgctgcgg tagcgcatgg
     1321 caaaagctgt gtacagcgct ttctggcttg cttaagaacc cttcgctgac agcaaaatag
     1381 acccccgacg tccccagcga taacattgcc tgattagcat caaccattcc cacaccaact
     1441 gcaccagctg cattgtcgcc accgcctgcg acaactggca ccgtcgccat accccacgct
     1501 ttcgcaactt caggtaacaa agcaccagta atttcgctgc cttcgtataa tgcgggcatc
     1561 tggtcacgag ataagtcgca agcctgcagc atgacgtcac tccagtcacg ctttgcgaca
     1621 tccagccaca tggtgccagc tgcgtcagac atatcgctgg caaactcccc cgtcatacgc
     1681 agacgcaagt aatctttcgg taataatact ttgtcgattt gacggaatat ctccggctca
     1741 tgccgctgaa cccatagcaa tttaggcgca gtaaatccgg gcatcatcag gttgccggta
     1801 atcactcgtg attgcggaac tctcgcttcc agcaaagtgc actcttgcgc acagcgcccg
     1861 tcgttccaca aaatggcagg gcgcaatacc cgttgttgag catccagtaa ggttgctcca
     1921 tgcatctggc cggcaatacc caatgcttta acgtcctgca gagaatgctg atcgcccaga
     1981 gctttcattg cgcgatcagt tgcctgccac cactgttccg ggtcttgttc cgaccagagt
     2041 ggatgcgggc gcgaaacggt cagcttttcc gtttgcgaat caaccacctc accctgctcg
     2101 ttgagcaaaa taacttttac gcccgaggtg ccaagatcta tcccgatata catatcgatc
     2161 gttccttaaa aaaatgcccg gtatcgctac cgataaccgg gccaacggac tgcacagtta
     2221 gccgttattt gtcgaacaga taatgattta ccagattttc cagttgctcc tggcgaccac
     2281 tctgatgcac cggagacaaa ttatgttcct gagcatattt ggctaaatct gccagtgaca
     2341 tttggccttt caggatttgc tggcccaatt cgctattcca gccggaataa cgctgcgcga
     2401 tgcgtttatc cagctcgcca tcttcaatca tgcgcgctgc aattttcagc gccagtgcca
     2461 tcgtatccat cgcgccgata tgaccgtaaa acagatcata tttatcagta ctttgacgac
     2521 gtactttggc atcgaagttc agaccaccgg tggtgaaacc gcctgctttg agaatttcat
     2581 acatcaccag cgcattctct tccacactgt tcgggaactg gtcggtgtcc cagcccagtt
     2641 gcgcatcgcc acggttggcg tcgacagaac cgaacaggcc aagcgcaatg gcggtggcta
     2701 tttcatgatg gaaagagtga cctgccagcg tcgcgtggtt agcttcaatg ttcagtttaa
     2761 tctctttttc cagaccaaac tgtttcagga agccatagac cgtcgcggca tcgtaatcat
     2821 attgatgttt agtcggttct tgcggtttcg gttcgataag caacgtgccc tggaagccga
     2881 ttttatgttt atgctcaacc accatctgca taaagcggcc cagttgttca cgctcctgac
     2941 gcaagtcggt atttaacagc gtttcgtaac cttcacgacc accccacagg acatagtttt
     3001 caccgcccaa tttatgggtt gcttccatcg ctgtaacaac ttgcgttgcc gcccagctga
     3061 agacttcagg atctgggttc gtcgccgcac ccgcgccata gcgagggttt gtaaagcagt
     3121 tagcggttcc ccacagcagc ttcacgccgc tctcttcttg cttggctgcc aggacatcaa
     3181 ccatttgcgc aaaattattg atgtactctt ttaacgacgc gccctcaggg gaaacatcca
     3241 catcgtggaa gcaataaaat ggcacatgta acttgtggaa aaactcaaat gcgacatctg
     3301 ctttacgctt cgccaacgcc agtgcctcac caggctgctg ccacggacga ttaaacgccc
     3361 ccacaccaaa catgtccgcc ccgttccagc agaaggtgtg ccagtagcag gcggcaaaac
     3421 gcaagtgctc ttccatacgc ttacccaaca ccagttcgtc gggattgtag tgacggaatg
     3481 ctaacgggtt tgaggatttt gagccttcat aacgaacgcg atcgagctgg tcaaaatagg
     3541 cttgca
//
LOCUS       DAZPDU010000291         3417 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZPDU010000291 DAZPDU010000000
VERSION     DAZPDU010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.295"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(198..866)
                     /locus_tag="ACHURS_004389"
     CDS             complement(198..866)
                     /locus_tag="ACHURS_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239954.1"
                     /translation="MDCRKLNIVLLSTVAVLLVVVILVLIKSLFVGSEGFEWGSVSDW
                     VSSIANLIMAGAALYAAWNAKNWFQTKIKENALNQVTKFWTNCDLFSLEMLKTYENIC
                     NLNNTEPDIEPKNFAHLVKTEREVIDLLNIKLASLKSELQSLTFWKVSPKDEAIFNNY
                     FFSNQIALGTLREAISFDINDIHDRYHYNVSYGKLLVDTFDSLIEAHATLKKDMNNLF
                     VFSN"
     gene            complement(999..1310)
                     /locus_tag="ACHURS_004390"
     CDS             complement(999..1310)
                     /locus_tag="ACHURS_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012125135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239955.1"
                     /translation="MSKDGYDPSITRGVQKFSHNGRWATVCYWGSALTEFHYGDRVFF
                     QNQYREYWLGTIERDCFVLTCETPLISVIDGLAYLSAEYRMNQSSEEGDWFCDQGELP
                     F"
     gene            1503..2054
                     /locus_tag="ACHURS_004391"
     CDS             1503..2054
                     /locus_tag="ACHURS_004391"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239956.1"
                     /translation="MNIDNWAIPITSLGGQLLGVELVSHVEREGIRQIGLWEEEVLHG
                     QLMLIESKAAWFRDNGLYCVITTGRGEPYEYLPFLRQMTRDMKVHNQQWLDDLGVHGG
                     SAIPLVTGDFEVARLNRRYTEENIDRLIFPVLIKSIRQYCEKVIVPVRDKTYYPILRE
                     AGVWAVQGEFRPMRFEQCEKLIG"
     gene            2805..3245
                     /locus_tag="ACHURS_004392"
     CDS             2805..3245
                     /locus_tag="ACHURS_004392"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239957.1"
                     /translation="MKITVDNISEMFAGTKELLIGGLITGIVGVLILTRVSGYEYVKV
                     PLMLVMIFLLTVVPGFIAMLFSPLTKVFAKIFDIKSSNILCRIFSFLYFILYMAITFL
                     VTNFIFTTAMSLHGGDGGLRACFAIGCFVVAVEQTRKFYSLLIK"
BASE COUNT         1042 a          614 c          702 g         1059 t
ORIGIN      
        1 aactactaac ccactatagg gtttacgaga tgataagaat ggaatttaac acctacgatg
       61 gtgatatata ttacttcctc tatttatata ggaggtacga aagggggagt actttaatga
      121 agaccttata aatcttaatg ttgagatgtt agttatagcg gtgaggatac cccaccgtct
      181 attgggagtt gtggactcta attagagaaa acgaataagt tattcatgtc ctttttcaag
      241 gttgcatgtg cttctattaa actatcaaat gtatctacta ataactttcc atagctgaca
      301 ttgtaatgat atctatcatg aatgtcattt atatcaaaac ttatcgcttc cctcaatgtt
      361 cctaaagcaa tttgattact gaagaaataa ttattgaata tagcttcatc ttttggcgat
      421 actttccaaa aggtaagtga ttgcaattcg ctttttaatg aggctaactt aatgtttaat
      481 agatcgataa cttctcgttc agttttaact aaatgtgcga aattttttgg ttctatatct
      541 ggttcagtat tgttaaggtt gcatatattt tcatatgttt tcaacatctc caacgaaaaa
      601 agatcgcaat tagtccagaa cttagtaact tgatttaaag cattttcctt tattttagtt
      661 tgaaaccaat ttttagcgtt ccatgctgca tatagggctg ctccagccat aatgagattg
      721 gcaatagaac taacccagtc actaaccgat ccccactcga aaccttcaga accaacaaag
      781 agtgatttta ttagtactaa aattacaaca accagtaata ctgctacggt cgataaaaga
      841 acgatattta attttctaca atccatcgaa agtagttcct gaacgcatta aaggggcaaa
      901 tactttacca tccgaaacac aatcaagtac actacccgtt cacgtactgc tcacatgttg
      961 ggtttgttat tttgttgggg tgagcagtac gtgtatcctc agaagggtag ttccccctga
     1021 tcacagaacc aatcaccttc ttcactgctt tgattcatcc ggtactcagc agatagatat
     1081 gctaacccat caataacact tattagtggt gtttcacagg taagtacaaa acaatctctc
     1141 tctatagtac ctaaccagta ttccctgtac tgattctgga agaataccct atccccataa
     1201 tgaaattctg ttagtgcaga tccccaataa cacacggttg cccagcgacc gttatgtgag
     1261 aacttctgaa cccctcgcgt aatggatgga tcatagccgt ctttgctcat ttgttaccct
     1321 cctctatgta ttactgtata tatgtacagt attgcagtca gtttcccaaa aatccagcac
     1381 ccctttgttt accactttga atgattaatt ctattgtaat gatcaataaa attgatcgat
     1441 ttaattgcca atgttttaaa aatgatcaat tatcaataac ttaaagcgta acgagcaaag
     1501 ttatgaacat tgataattgg gcaataccga taacaagttt gggtggtcag ttgctggggg
     1561 ttgagttggt cagccatgta gaacgcgagg gaatcaggca gatcggccta tgggaagaag
     1621 aagtacttca tgggcaacta atgcttattg aaagcaaggc ggcatggttc agggataacg
     1681 gtttgtattg tgtgattact actggccgtg gtgaaccata cgagtactta ccattccttc
     1741 ggcagatgac aagggatatg aaagtacata accagcagtg gcttgatgat ctcggtgtac
     1801 atggtggttc tgctattcct ctggttacag gtgatttcga ggttgcccga ttaaatagaa
     1861 ggtacacaga agagaacatt gaccgtctga tctttccagt actcataaaa agcattcgcc
     1921 agtactgcga gaaggtgatc gtacccgtca gagataaaac gtattaccca attctcagag
     1981 aggccggtgt atgggcggta cagggagagt tcaggccaat gcgttttgag caatgtgaaa
     2041 aattaatagg ataacaataa tgaaaaaatt actaattggg ttactgattg cccttagtac
     2101 tggatgtact actacgcaca gcttcatgga taaagcttac gttggctatg tagatgacaa
     2161 ggccgtagta ctgtttgatg caggaacacg taatggggtg aagtactacc gtcctcagtt
     2221 ccagagtgat tgcggtcgca tctataacaa gatctatcat gatgacaata caagtactgg
     2281 atgcctcata cgagattccc gcattgctga actacatccc tatagagagc atgtaagtaa
     2341 ggtagtacca catcagaccg atagtgttca cgattggtta gaaggggtca attcaccatc
     2401 ccatatcctc gacaatctat agggtacgta tgggaaaggt aatggtgatt ttgttagtac
     2461 tggctggggt actcttcaac attgcaggtg aggatgcacc ccagccacag tacacggaca
     2521 atgagaacca gaacgctatg gatttcgcac agtcactaat caagggtaag tacccatgcg
     2581 atatggtgac tatggatagt actgagaatg gtacaggacg tattgatgta cggtgtgatg
     2641 gtaagtacca gtatgagatc atccggcatg atggacaatg gcaggtagta tcgaagtaat
     2701 ccgaaaagtt tagtgcttag gctgtatggt gagaatctac agggcttttt ctttatgcgg
     2761 tgataagatc ggagtgaact ttttatgaag gaatagttag tagaatgaaa attacagtcg
     2821 ataatatctc tgaaatgttt gcaggaacta aagaactact aatcggtggc ttaataacag
     2881 gtatcgtcgg agtacttatt ttaactcgtg ttagtggtta tgaatacgtt aaagtacctt
     2941 taatgttagt aatgatattc ttactaactg tagttcctgg ttttattgct atgctttttt
     3001 caccactaac gaaagtattt gcaaagatat ttgatattaa aagcagtaat atactttgcc
     3061 gcattttctc atttctatat ttcatcttgt atatggctat tactttcttg gtgactaatt
     3121 ttatattcac taccgcaatg tcattacacg gcggggatgg tgggttgaga gcatgttttg
     3181 caattggctg ttttgtcgtt gcagttgagc agacaagaaa attctatagt ttgcttatca
     3241 agtaaagggc aagtactaaa gaaatgtgta tttgatgaag ggtaagtact ttcatcaaat
     3301 acgcaacaaa ttacaaggtg agcatttcat cacaattgtt ttaattaacg atattttaaa
     3361 ttattttacg taattgaaac aaaatcagcc atatcgcttg ttaattggta ttgagac
//
LOCUS       DAZPDU010000292         3415 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZPDU010000292 DAZPDU010000000
VERSION     DAZPDU010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.296"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..229
                     /locus_tag="ACHURS_004393"
     CDS             <1..229
                     /locus_tag="ACHURS_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFR1239958.1"
                     /translation="MSTGWLANTSTDGNHSHSLSGTAASAGAHAHTVGIGAHTHSVAI
                     GSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            226..1128
                     /locus_tag="ACHURS_004394"
     CDS             226..1128
                     /locus_tag="ACHURS_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HFR1239959.1"
                     /translation="MITLILSAPVTEMAEAFKRVFANADNVNIVGKPFETIREFDCMV
                     SAANSFGLMDGGVDAAITAFFGTQLQSRVQNHILREYLGEQPVGSAFVIETGHNHHPW
                     LVHAPTMRVPLTIDGTDAVYNATWAALLAIFQHNKHATTDRKIKTVVFPAMGAGCGQV
                     PFESVARQMKQAWDNFNKKTESINWEYAQSRQSAVFGTYAYCPGNSVCRYADTKYIGC
                     GDYRTYCSRSGQVCINPEHQADDVLIQHQANNRFRPGSHIHRINPENPVGNVISGAHS
                     HGSSIVIGAHTHTLNKQYSISDIK"
     gene            1149..1721
                     /locus_tag="ACHURS_004395"
     CDS             1149..1721
                     /locus_tag="ACHURS_004395"
                     /inference="COORDINATES: protein motif:HMM:NF014467.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR1239960.1"
                     /translation="MSEQPRTITIYNLLAGTNEFIGEGDAYIPPHTGLPANSTNIAPP
                     DIPAGFVAVFNSDDASWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSPEGEY
                     QKWNGTAWVKDAEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEEENSLL
                     EAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            complement(1837..2085)
                     /locus_tag="ACHURS_004396"
     CDS             complement(1837..2085)
                     /locus_tag="ACHURS_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR1239961.1"
                     /translation="MRIEICIAKEKMTKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(2147..3244)
                     /locus_tag="ACHURS_004397"
     CDS             complement(2147..3244)
                     /locus_tag="ACHURS_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1239962.1"
                     /translation="MAYYNIEKRLKSDGTPRYRCNVIIKEKGVITYRESKTFPKHAHA
                     KTWGTQKVMELDLYGIPSSNAVDGLTVRDLLHKYLNDPNAGGKAGRTKRYVLELLMDS
                     DISAIKLSELTENDVIEHCRLRNNAGAGPATVSHDVSYLGSVLDAAKPVYGINYTSNP
                     AKSARPYLLKLGLIGKSNRRNRRPASDELDMLIEGLQQRSTHKCSKIPFVDILKFSVW
                     SCMRIGEVCRLRWEDLDQEQKSILVRDRKDPRKKEGNHMKVALLGEAWDIVQRQPKKS
                     EFIFPYNSTSVTAGFQRVRSKLGIKDLRYHDLRREGASRLFEAGFSIEEVAQVTGHRS
                     LNVLWQVYTELYPKSLHNRFEELQRSRNKTS"
     gene            3333..>3415
                     /locus_tag="ACHURS_004398"
     CDS             3333..>3415
                     /locus_tag="ACHURS_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000543817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HFR1239963.1"
                     /translation="MHDNHETQKINQTSVMPEKTGVYWNSR"
BASE COUNT          930 a          744 c          775 g          966 t
ORIGIN      
        1 tatgtctacg gggtggttag cgaataccag tacagatggt aatcatagcc attcactgtc
       61 cggcacagca gcatctgcag gtgcacacgc gcatactgtc ggtattggtg ctcatacgca
      121 ctccgttgcg attggttcac atggacacac catcaccgtt aacgctgctg gtaacgcgga
      181 aaacaccgtc aaaaacatcg catttaacta tattgtgagg cttgcatgat tacgctcatt
      241 ctttctgcac cagtaacaga aatggctgaa gcatttaagc gggtatttgc aaacgcagat
      301 aatgtgaata ttgtcggaaa gccatttgaa acaatcaggg aatttgactg catggtaagt
      361 gcggcaaata gtttcggcct gatggatggc ggtgttgatg ccgccattac cgcattcttc
      421 ggtactcagt tacagtcccg cgttcagaat catattcttc gtgaatatct aggcgagcag
      481 cctgtaggtt ctgcatttgt tattgaaacg gggcataatc atcatccctg gctggtacat
      541 gcgccaacaa tgcgtgttcc gctgacaatt gacggaacag acgctgtata taacgcaacc
      601 tgggccgctc tgcttgccat ctttcagcac aataaacacg caacgacaga caggaaaata
      661 aaaacggtgg tattccctgc aatgggagcc ggatgtggtc aggtgccgtt tgaaagtgtt
      721 gcccggcaga tgaagcaggc atgggataac tttaataaaa aaacagaatc aattaactgg
      781 gaatacgcac aatcccgcca gtcggcagta tttggcacat atgcatactg tccgggtaat
      841 tctgtttgcc gttatgcgga tactaaatat attggatgcg gcgattatcg gacgtattgc
      901 tcacgttccg ggcaggtctg tattaaccct gaacatcagg ctgatgatgt actgatacaa
      961 catcaggcta ataaccggtt tcgccctggt tcgcatatac accggattaa tccagaaaat
     1021 cccgtaggta atgtcatctc tggcgcacat agccacggaa gtagcatcgt tattggtgct
     1081 cacacccata cgctcaataa acaatattct atctctgata ttaagtagag gtgaacatgg
     1141 atttcagaat gagtgaacaa ccacggacta taacaattta taatctgctg gccggaacta
     1201 atgaatttat tggtgaaggt gacgcataca ttccgcctca tacaggtctg ccagcaaaca
     1261 gtaccaatat tgcaccgcca gatattccgg ctggcttcgt ggctgttttc aacagtgatg
     1321 atgcatcgtg gcatcttgtt gaagaccatc ggggtaaaac agtttatgac gtggcatcag
     1381 gggacgcgtt atttatttct gaactcggtc cgttaccgga aaatgttacc tggttatcgc
     1441 cggaagggga atatcagaag tggaacggca cagcctgggt gaaagatgca gaagcagaaa
     1501 aactattccg gatccgggaa gcggaagaaa caaaaaacag cttgatgcag gtagccagtg
     1561 agcatatagc gccgcttcag gatgctgtag atctggagat cgcaacggag gaagaaaact
     1621 cgttgctgga agcctggaag aagtatcggg tgttgctgaa ccgtgttgat acatcaactg
     1681 cacctgatat tgagtggccg gaagaaccag acacaatgta agcgaaaaag aaaaaccgca
     1741 gacacgacgt atgcaggacg tgctgcggtt ggctggtgaa ctttcgatag tgcgagtatt
     1801 gaatgatttc cagccgttac cgattttacg tgtttattag tgaacaaacc actcgtcagc
     1861 agactcccag gtatctttca gagtctcctg aacaaatgtt tttgcagaat ctttatctgc
     1921 ggtgcgtgta acagaaagcc catcgttgct ggtggctttt acgatcacct ctacatcgtc
     1981 ataacgttta ctgatgcgtc gggttaattc ttcctttaac gcatccacag caccgtttgg
     2041 cattttagtc attttttctt tggctatgca gatctcaata cgcataaaag tccctctata
     2101 ctgtgtttgt atacagtatt atttttaact gtatggataa acagtgtcaa gaggtcttat
     2161 ttctgctcct ttggagctct tcaaaacgat tatgtaaaga tttcggatac agttcggtat
     2221 atacctgcca tagcacgttt aatgaacgat gccctgtaac ctgagcgact tcctcaatac
     2281 taaaaccagc ctcaaataag cgacttgccc cttctctacg caaatcatgg tatcgcagat
     2341 ctttaatacc taatttgctt cttaccctct gaaatcctgc agtaacagaa gtgctgttat
     2401 atggaaaaat gaattccgat tttttgggct gtcgttggac gatatcccag gcttccccaa
     2461 gcaaggctac tttcatgtgg ttgccttcct ttttgcgtgg atctttcctg tctcttacga
     2521 gtatagattt ttgttcctgg tcgagatctt cccatcgtaa ccggcatact tctccgatcc
     2581 gcatacagga ccacacagaa aatttgagga tatcaacgaa cggaattttt gagcatttat
     2641 gagtagatcg ttgttgaagg ccttcaatga gcatgtccag ttcatcagat gctggtctac
     2701 gattacgacg gtttgattta ccaatcaaac caagtttaag tagatatggg cgagcgcttt
     2761 tcgctgggtt tgatgtgtaa ttaattccat atacaggttt ggcagcatcc agaacactgc
     2821 caagataact aacatcgtgg ctaactgtag ctggacctgc accagcgttg tttcttagcc
     2881 tgcaatgttc aattacgtca ttttctgtca gttcagatag tttgatcgcg gagatgtcac
     2941 tatccataag cagttccagc acatatcttt tagtacggcc tgctttacct ccggcatttg
     3001 ggtcatttaa atatttgtgt agtaagtcac ggactgtaag tccgtcaact gcatttgatg
     3061 atggaatgcc atatagatct aattccatca ctttctgtgt gccccatgtt ttggcatgag
     3121 catgtttagg gaatgttttg ctttccctgt aagtgataac acctttttct ttgataatca
     3181 cattacagcg atagcgtggt gtgccatcgg attttagtcg tttctctatg ttatagtacg
     3241 ccattacacg acctcgttat ttcgggttcc cataaaacat gggaacctgt gcgggaacct
     3301 aatgcgagaa aaatagcctg aaatgttcaa aaatgcacga taatcatgaa acacaaaaaa
     3361 ttaatcaaac cagcgtgatg cctgaaaaaa ctggtgttta ctggaattct cggtt
//
LOCUS       DAZPDU010000293         3410 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZPDU010000293 DAZPDU010000000
VERSION     DAZPDU010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.297"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            443..1609
                     /gene="sopA"
                     /locus_tag="ACHURS_004399"
     CDS             443..1609
                     /gene="sopA"
                     /locus_tag="ACHURS_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid-partitioning protein SopA"
                     /protein_id="HFR1239964.1"
                     /translation="MSLKKLLQDCVTRGQEMTQAIAIAQFGDDSPEARRITRRWGITE
                     VADLIGVTPQAIRDAEKSGRLPAPDFEMRGRIERRAGYTIDQINSMRAVFGNPNQRPQ
                     DKNPVALAVMSHKGGVYKTSSAVHQAQWLALQGHRVLLVEGNDPQGTASMYHGYVPDL
                     HIHAEDTLLPFYLGERDNAEYAIKPTCWPGLDIIPSCLALHRIETDLMQYHAQGKLPH
                     PPHLMLRAAIESVWDNYDVIVIDSAPNLGTGTINVVCAADVIVVATPAELFDYASVLQ
                     FFTMLLDLLESVDLGGFEPTVRLLLTKYSLTTGNQSRWMEEQIRNTWGSMVLRQVVRV
                     TDEVGKGQIKMRTVFEQAANQRSTLNAWRNAVSIWDPVCREIFDDLIKPRWENE"
     gene            1609..2574
                     /locus_tag="ACHURS_004400"
     CDS             1609..2574
                     /locus_tag="ACHURS_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family plasmid partition
                     protein"
                     /protein_id="HFR1239965.1"
                     /translation="MKQRSLLKNAPDINRIMSNNHHAPEHQPVSPMVGDLKRQLSSLT
                     GNSITLPVCGRNVNFKLETIPADKVEMATMVWLGNERDQDLLNESSLADLIPSFLTSG
                     QQNPAFARKVSGIVEVADGSRRRKAAILTGCDYRVLIGDLDDEQMHWLSQIGNDYRPI
                     SAYERGKRYLRKLNDFDGNVKALAEAENIDRNIITRCINTAGLPKDILAIFNHPGELS
                     ARAGDSLFKVYKKNMAAMSNAAHHLLAIKKNGEDLEASRIIQILSDSVLVGDEEKTKD
                     EKKYGEGITARYKGNFVTIKIDSRKISKEILSRIELLLEEQSTDN"
     gene            complement(2607..3182)
                     /locus_tag="ACHURS_004401"
     CDS             complement(2607..3182)
                     /locus_tag="ACHURS_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR1239966.1"
                     /translation="MAFRMSEQPRTITIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     EGEFQKWNGTAWVKDTEAEKLFRIREAEETKNNLMQVASEHIAPLQDAADLEIATEEE
                     NSLLEAWKKYRVLLNRVDTSLSPDIEWPSQP"
     gene            complement(3182..>3410)
                     /locus_tag="ACHURS_004402"
     CDS             complement(3182..>3410)
                     /locus_tag="ACHURS_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFR1239967.1"
                     /translation="MSTGWLANTSTDGNHSHSLSGTAASAGAHAHTVGIGAHTHSVAI
                     GSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
BASE COUNT          984 a          671 c          727 g         1028 t
ORIGIN      
        1 attatgtttc gttatgttct tgattggtgt tttcatttga tgtaatgttc tcagtttgcg
       61 tactgtttta tttaacattt gtttttattc tttatttatg ttctttattg tgtttgtgtt
      121 attacttgtt ttttatgttc tttatttgtg ttcttatgtt ggtaaggttg ggagaactaa
      181 tagagaaaac gtatctcatt gatgattctt atcatgtttc tctagtttta tatgatttcc
      241 tttcttaaga aaggtcataa atatcaatta gttatgatta tattgttgtt cgttttatgc
      301 atttcgggag gggaaaaaaa gatgatttag ctcaaatatt cgcctttgcg tttttcgttt
      361 tgctttgcct tttttatgca aatttgctag aaataagcaa agatttaatt attaagcaaa
      421 gttacaagct ggagatgaca gtatgtcttt gaaaaagtta ttgcaagatt gtgttacgag
      481 aggtcaagaa atgacccaag caattgctat cgctcaattc ggtgatgata gtcctgaagc
      541 tcggcgtatc acccgccgat ggggtataac tgaagtagca gatttaattg gtgtaactcc
      601 tcaagcaatt agagatgctg agaagagcgg gcgtttacct gcacctgact ttgaaatgag
      661 aggaagaatt gaacgtcgtg ctggttatac catagatcaa atcaacagta tgcgagcagt
      721 ttttggcaac ccaaaccaac gaccacaaga taaaaaccct gttgcgcttg ccgttatgtc
      781 acacaaaggc ggcgtttata aaacatcgtc tgcggtacat caagctcaat ggttagcatt
      841 acaaggtcat cgtgttcttt tggttgaagg taacgatcct caaggcacgg cctctatgta
      901 tcacggttat gttcctgatt tacatatcca tgccgaggat actttactgc cattttactt
      961 aggagaacgc gataacgctg aatatgctat aaagccaaca tgttggcctg ggctagacat
     1021 tatccctagc tgcctagctt tacatcgtat agaaacagat ctcatgcaat atcatgctca
     1081 gggtaaattg cctcatcctc cacaccttat gctgcgcgca gctattgaat cagtttggga
     1141 taactacgat gttatcgtta tagatagtgc tccgaacctc ggtacaggaa ccattaacgt
     1201 tgtttgtgcc gctgacgtta ttgttgttgc tacacctgca gaattatttg attacgcttc
     1261 tgttctacag tttttcacaa tgcttctcga tctattggaa agcgttgacc taggtggatt
     1321 tgagccaacc gttcgcttac ttctgactaa atatagtctc actacaggga atcaatcacg
     1381 atggatggaa gaacaaataa gaaatacatg gggatcaatg gtattacgtc aggttgtacg
     1441 cgttaccgat gaagtaggta aaggccagat aaaaatgaga acagtatttg aacaagcagc
     1501 aaaccaacgt tcaacattga atgcatggag aaatgctgtt tctatatggg accctgtttg
     1561 ccgagaaatt tttgatgatc taataaaacc tcgctgggag aacgaataat gaaacagcgc
     1621 tctcttctaa aaaatgctcc agatattaat cgcattatga gtaataatca tcatgcccct
     1681 gaacatcagc ccgtatcacc tatggttggt gatttaaaac gtcaactgag ttcattaacc
     1741 ggtaacagca taactttgcc agtatgcgga cgtaatgtta actttaagtt agagacaatt
     1801 cccgcagata aagttgaaat ggcaacaatg gtatggctag gtaatgaacg cgatcaagat
     1861 ttattaaatg aatcctctct agccgattta atcccatctt ttctaacgtc tggacaacaa
     1921 aatccagctt tcgccagaaa agtatctgga atcgttgaag tcgctgacgg ctcacgtaga
     1981 agaaaagcag cgatattaac aggatgtgac tatcgcgtct taattggtga tctcgacgat
     2041 gaacagatgc attggttatc ccaaattggt aacgattata ggcctattag tgcatacgaa
     2101 agaggaaaga gatacttacg caagctaaat gactttgatg gtaacgttaa agcattagcc
     2161 gaggctgaaa atatagatag aaatattatc actcgctgta ttaatacagc tggtctacca
     2221 aaagatatat tagctatctt caatcatcca ggagaactgt cagctcgtgc tggagattct
     2281 ttgtttaagg tttacaaaaa aaacatggct gccatgagca atgctgctca tcatcttctg
     2341 gcaattaaaa aaaacggcga ggatttagaa gcttcacgga ttatacaaat attatctgac
     2401 tctgttctgg ttggcgacga ggaaaaaaca aaagacgaga aaaaatatgg ggaagggatc
     2461 acggctagat ataaaggtaa ttttgtaact ataaaaattg atagccgcaa aatatctaaa
     2521 gaaatactaa gcagaattga gttgttacta gaggaacaat ctactgataa ttaagaacta
     2581 tcccctgcat tgcaggggat attgatttat ggttgacttg gccactcgat atctggagat
     2641 agtgatgtat caacacggtt cagcaacacc cgatacttct tccaggcttc cagcaacgag
     2701 ttttcttcct ccgttgcaat ttccagatct gcagcatcct gaagcggcgc aatatgctca
     2761 ctggctacct gcatcaggtt gttttttgtt tcttccgcct cccggatccg gaacagtttt
     2821 tctgcttccg tatccttcac ccaggctgtg ccgttccact tctgaaactc cccttccggg
     2881 gataaccagg tgacattttc cggtaatggg ccaagttcag aaataaataa cgcgtcgccg
     2941 gaagccacgt catagacggt ttttccccga tggtcttcaa caagatgcca cgatgcctca
     3001 tcactgttga aaacagccac gaagccagcc ggaatatctg gcggtgcaat atcggtactg
     3061 tttgctggca gacctgtatg aggcggaatg tatgcgtcac cttcaccaat aaattcatta
     3121 gttccggcca gcagattata aattgttatg gtccgtggtt gttcactcat tctgaatgcc
     3181 attatgcaag cctcacaata tagttaaatg cgatgttttt gacggtgttt tccgcgttac
     3241 cagcagcgtt aacggtgatg gtgtgtccat gtgagccaat cgcaaccgag tgcgtatgag
     3301 caccaatacc gacagtatgc gcgtgtgcac ctgcagatgc tgctgtgccg gacagtgagt
     3361 ggctatgatt accatctgta ctggtattag ctaaccaccc cgtagacata
//
LOCUS       DAZPDU010000294         3400 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZPDU010000294 DAZPDU010000000
VERSION     DAZPDU010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.298"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            719..1405
                     /locus_tag="ACHURS_004403"
                     /pseudo
     CDS             719..1405
                     /locus_tag="ACHURS_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554952.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1738..2613
                     /locus_tag="ACHURS_004404"
     CDS             1738..2613
                     /locus_tag="ACHURS_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HFR1239968.1"
                     /translation="MRKFILISMITLASVSLGACRQNVTIKQDAPGQKAFLTDGQIYD
                     LHSGKIISSSELLADLATARHLIIGEKHDNAEHHQIELWLIQNLLIQRPQGSVLLEML
                     TSEQQPRVNQVKCWLKDNPVVRDSRVQELLNWQKGWSWEMYGDIVMQLLRGPYPLLNA
                     NIGREQMLALYKKNEFPKGKKSTAPVVQEALRETIISMHEGNLESQQLTSMLSIQQQR
                     DRYMARQLLSAPVPSLLIAGGYHASKSMGVPLHMEDLATGTHPVVLMLAEKGMNITVD
                     HADYVWFVAPDTTKR"
     gene            2621..3397
                     /locus_tag="ACHURS_004405"
     CDS             2621..3397
                     /locus_tag="ACHURS_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HFR1239969.1"
                     /translation="MMNILHFSQSVKWSSWFICSLLLHGLIFLAFIWRFSEVQPAMSP
                     APAIMLQWAEIIEAPSSPLSLPVGIAQQESAVTEEKQQTEDRQQRPVTEDSDATIEIT
                     RKKKSSDGEKKKTRPPRKIKAQTSDSNPTAVSSNAAPQALVESSRIAAPFNSDSTKRD
                     NSEASWESRVKGHLNRYKRYPGDARKRARTGTAVVTFTVNTEGTIVSSFLEISSGTLS
                     LDREAIAVLERAQPLPKPPPEILEGGLFKVKMPITFKLKE"
BASE COUNT          933 a          796 c          812 g          859 t
ORIGIN      
        1 tctcctttcc attggttaat ttatcggaaa ttcatcccca ctaactaaaa cgatccacac
       61 aataaaacta aatctaattg caggcaatca attccagtcg atatatcatc tgtatggaaa
      121 tcatcgctca tcactcatcg cagcctgaaa gcccagagca acggggcagg tctgcatata
      181 agtggttaca cggcagaatt ccagagggtt ctgtcactaa attcagtcac tgacaaattt
      241 gcatgatgtt gaatagacga gattttgtgt cgaaatagtt gtttcagtaa ctgtcactca
      301 atcggtggtt tatgtgacta tattcgcaaa aactggtcgc agaaaagtcc tgttttcagc
      361 ttggaaaccc ggtaatcaga ataatggata ggggaacaaa aatctctgta tagaacgctc
      421 cattgaggga gagaaaacgt gtcattttga cccgttcggt attcagaaca cacaaaaccg
      481 atcgcgggtc gctttcgtgc atagttatgc aacgcgctaa aaacgattct gacgcatttt
      541 ttatgtttac cctgtttttt cttgtctttt tgagtttttt gcgtcagaga gcgtctgaga
      601 gggcacgtac aacgggagtt atggtaaatg gatcgggttt tcgggaaggt tcgacaggat
      661 ttgccgttgg gtgtagtgta agcgactgaa aaacaaacgc ccctgaaatc atgtccagat
      721 ccggcagaac cagcctcatg cgcagttctg gctggttttt gacgttgatc gcgagggggc
      781 agcgattgac tggagcgacc ggaacgctcc cgcgccgaac atcaccgtaa aaaatcctgt
      841 gaacggtcat gctcacctgc tctacgcgct caatatcgcc gtgagaaccg cgccggacag
      901 ctcggttaag gcgctgaaat acgccgctgc gattgaacgt tcgctgtgtg agaaactttg
      961 tgctgatgtg aactacagcg gactgatttg taaaaatccg ttccacctgg aatggcaggt
     1021 gatggagtgg cgagaggaag cctataccct cgatgaactg gctgattatc tcgatttgag
     1081 cgcctcagcg cgccgtagca tcgataaaca ttacgggatg gggcgaaact gccacctgtt
     1141 cgaaatgacg cgcaaatggg cttacagggc gattcgtcag ggctggcccg aattctcaca
     1201 gtggcttgat gccgtgattc agcgtgtcga aatgtacaac gcatcgcttc ccgttccgct
     1261 ttcaccgcct gaatgtcggg ctattggcaa gagcattgcg aaatacacgc acaggaactt
     1321 cacgccggaa actttcgcac agtatgtggc tgatacgcac acattaacgt acgttttcgt
     1381 tccattggcc ctcacccccc gataacgacc caggtagcgc gccgtattcc ccccgccggc
     1441 cgttttcttc gacatgtaca tcaggcgcgg aactcatcgg ggcgcgggaa tgaaatccca
     1501 ttcagactcc ggatagattc aagcaagccc acttatcgtc aaaatcggtg ttgcaaaaac
     1561 gggagtgacc atagatccgt tttccaagcg gaccccataa tatcggctgg taaacatcac
     1621 tcgctagtgc attctttttg ttggtaatga taatcattta cattcattct ttgatgtttt
     1681 atcataatta atgttttgtc aataaatata tggaaacata ttgtttcaga tgggaatatg
     1741 agaaagttca tccttatttc tatgataacg ttagctagtg ttagtctggg agcctgcaga
     1801 cagaatgtca ccataaagca ggatgcgcca gggcagaaag ctttcctgac tgacgggcag
     1861 atttacgatt tgcacagtgg caaaattatt tctagcagcg agcttttggc ggatcttgct
     1921 accgctcgac atcttattat tggtgaaaaa cacgataatg ccgagcatca tcagattgag
     1981 ctttggctta ttcaaaattt gctcatccag cgaccacagg ggagtgtgtt gcttgagatg
     2041 ttgacgtcag agcaacagcc ccgagttaac caagttaaat gttggctgaa agataatcct
     2101 gtggttcgag atagccgtgt tcaggaacta cttaactggc aaaaagggtg gtcctgggag
     2161 atgtatggcg acatcgtcat gcagctatta cgtgggcctt acccgctgct caatgccaac
     2221 attggccggg agcagatgtt ggcactgtac aaaaaaaacg aatttcctaa aggaaaaaaa
     2281 tccactgcac ctgtggtaca agaagctctg cgtgaaacca tcatatctat gcatgaaggc
     2341 aaccttgaaa gccagcaact gacgtcaatg ctctctattc agcagcagcg ggatcgctac
     2401 atggcccgcc agcttctgag tgccccggta ccgtcgttgt tgattgcagg tggttatcac
     2461 gcgtcaaaaa gcatgggcgt accgttacac atggaagatc ttgccaccgg cacccatcca
     2521 gtggtgttga tgcttgcaga aaaaggaatg aacatcacag tagatcatgc tgactacgtg
     2581 tggtttgtag cgccagatac aacaaaaagg taaaccatgg atgatgaata ttctccactt
     2641 ctcacagtcg gtaaaatgga gcagctggtt tatctgttct ctactgcttc atggactgat
     2701 tttccttgcg tttatctggc gtttttccga agttcagccc gctatgtctc ctgcaccggc
     2761 gatcatgctg caatgggcag aaattataga ggctccttct tctccacttt cgcttccggt
     2821 gggaatcgct caacaggagt ccgcagtgac agaagaaaag caacaaactg aggacagaca
     2881 acaacgtcct gtcactgaag acagtgatgc aaccattgaa ataactagga agaaaaagtc
     2941 atccgatgga gaaaagaaaa agacacgtcc acctcgtaag ataaaggctc aaaccagcga
     3001 cagcaacccc acagccgttt ccagtaatgc tgcacctcag gctctggtcg aatcgtcccg
     3061 tattgctgcg ccttttaaca gtgactcaac aaagcgagat aattctgagg catcttggga
     3121 aagcagagta aaaggccacc taaaccgtta taaacgttac cctggcgatg ccagaaaacg
     3181 tgcacgaacc ggcacagccg tcgtgacttt taccgtaaac acggaaggca ctattgtcag
     3241 tagtttcttg gaaatctctt caggaaccct ttctcttgac cgcgaagcta ttgctgtttt
     3301 agaacgcgct caaccacttc ccaaaccacc gcctgaaata ttggagggag gattatttaa
     3361 agtaaaaatg ccgattacct ttaagttgaa ggagtaaagg
//
LOCUS       DAZPDU010000295         3378 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZPDU010000295 DAZPDU010000000
VERSION     DAZPDU010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.299"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            143..1108
                     /gene="dusB"
                     /locus_tag="ACHURS_004406"
     CDS             143..1108
                     /gene="dusB"
                     /locus_tag="ACHURS_004406"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFR1239970.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            1134..1430
                     /gene="fis"
                     /locus_tag="ACHURS_004407"
     CDS             1134..1430
                     /gene="fis"
                     /locus_tag="ACHURS_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFR1239971.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            1516..2400
                     /gene="yhdJ"
                     /locus_tag="ACHURS_004408"
     CDS             1516..2400
                     /gene="yhdJ"
                     /locus_tag="ACHURS_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFR1239972.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIVECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            2484..2663
                     /locus_tag="ACHURS_004409"
     CDS             2484..2663
                     /locus_tag="ACHURS_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFR1239973.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVYP
                     LKLVNCDELNFQDRM"
     gene            complement(2666..3328)
                     /gene="acrS"
                     /locus_tag="ACHURS_004410"
     CDS             complement(2666..3328)
                     /gene="acrS"
                     /locus_tag="ACHURS_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFR1239974.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTFNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDGMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
BASE COUNT          924 a          785 c          799 g          870 t
ORIGIN      
        1 gagtaattat ctcaccacgc tcattttaaa tgcaattctt tgatccatct cagaggattg
       61 gtcaaagttt ggcctttcat ctcgtgcaaa aaatgcgtaa tatacgccgc cttgcagtca
      121 cagtatggtc atttcttaac tcatgcgcat cggacaatat cagctcagaa atcgcctgat
      181 cgcagcgccc atggctggca ttacagacag accttttcgg acgttgtgct acgagatggg
      241 agccggattg acagtatccg agatgatgtc ttctaaccca caggtttggg aaagcgacaa
      301 atctcgttta cggatggtgc acattgatga acccggtatt cgcaccgtgc aaattgctgg
      361 tagcgatccg aaagaaatgg cagatgcagc acgtattaac gtggaaagcg gtgcccagat
      421 tattgatatc aatatgggtt gcccggctaa aaaagtgaat cgcaagctcg caggttcagc
      481 cctcttgcag tacccggatg tcgttaaatc gatccttacc gaggtcgtca atgcagtgga
      541 cgttcctgtt accctgaaga ttcgcaccgg ctgggcaccg gaacaccgta actgcgaaga
      601 gattgcccaa ctggctgaag actgtggcat tcaggctctg accattcatg gccgtacacg
      661 cgcctgtttg ttcaatggag aagctgagta cgacagtatt cgggcagtta agcagaaagt
      721 ttccattccg gttatcgcga atggcgacat tactgacccg cttaaagcca gagctgtgct
      781 cgactataca ggggcggatg ccctgatgat aggccgcgca gctcagggaa gaccctggat
      841 ctttcgggaa atccagcatt atctggacac tggggagttg ctgcccccgc tgcctttggc
      901 agaggttaag cgcttgcttt gcgcgcacgt tcgggaactg catgactttt atggtccggc
      961 aaaagggtac cgaattgcac gtaaacacgt ttcctggtat ctccaggaac acgctccaaa
     1021 tgaccagttt cggcgcacat tcaacgccat tgaggatgcc agcgaacagc tggaggcgtt
     1081 ggaggcatac ttcgaaaatt ttgcgtaaac agaaataaag agctgacaga actatgttcg
     1141 aacaacgcgt aaattctgac gtactgaccg tttctaccgt taactctcag gatcaggtaa
     1201 cccaaaaacc cctgcgtgac tcggttaaac aggcactgaa gaactatttt gctcaactga
     1261 atggtcagga tgtgaatgac ctctatgagc tggtactggc tgaagtagaa cagcccctgt
     1321 tggacatggt gatgcaatac acccgtggta accagacccg tgctgcgctg atgatgggca
     1381 tcaaccgtgg tacgctgcgt aaaaaattga aaaaatacgg catgaactaa ttcaggttag
     1441 ctaaatgctt gattaaaaag gcgctactcg gcatggggaa gcgccttttt tataggtgtc
     1501 acaaagggag tgaccatgag aacaggatgt gaaccgaccc ggtttggtaa tgaagctaag
     1561 accattattc acggtgatgc ccttgccgaa cttaaaaagc tacctactga aagcgtcgat
     1621 ctgatctttg ccgacccacc gtataacatc ggtaaaaatt ttgatggtct gatcgaagcc
     1681 tggaaagaag atctgtttat cgactggctg tttgaagtga ttgtagagtg ccaccgcgtt
     1741 ctgaaaaagc agggcagcat gtacattatg aacagtacgg aaaacatgcc ctttatcgat
     1801 ctccagtgcc gcaagctttt taccatcaaa agtcgcatcg tctggtcata tgacagttct
     1861 ggagtacagg cgaaaaaaca ctacggctcc atgtacgaac ccatcctgat gatggtgaaa
     1921 gacgcaaaga actacacatt caacggtgat gctattctgg tagaagccaa aaccggatcg
     1981 cagcgcgcgt tgatcgatta tcgcaaaaat cctccacagc catacaatca tcaaaaagta
     2041 ccgggtaacg tctgggattt tccgcgcgtg cgttatttga tggatgaata tgagaaccac
     2101 ccgacgcaaa aaccggaagc cttactgaaa cgcattattc tcgcctcttc caacccaggc
     2161 gatatcgttc tcgacccgtt tgccggtagc tttactaccg gtgccgtagc catcgccagc
     2221 ggacgaaaat tcattggtat tgagatcaac agcgagtaca tcaaaatggg gcttcgacgg
     2281 ctggatgtcg cgtcgcatta ctccgcggaa gaactggcga aagtgaaaaa aagaaagacc
     2341 ggcaacctgt caaaacgaag ccggttaagc gaagttgacc ccgatctcat tgcaaagtaa
     2401 aggaagtgta agcctggttt tcagattatt catttcgtgt atatttcctg ccagacttgg
     2461 ttaaacatgc acaggctcag gtaatgattc gcaagtattg gtggctcgtc gttttcgctg
     2521 tcttcgtttt tctgtttgat actttactga tgcagtggat tgaactgctg acaacagaaa
     2581 cagacaaatg ccgtaatatg aactcagttt atccactaaa actggttaac tgtgacgaac
     2641 tgaattttca ggacagaatg tgaatttaca tgacacttaa ttcattcgtt tgatgaatta
     2701 atttcgttat gttttcatct ggcatgaaca ttcttaatac gttatcgacc agcgcgggtg
     2761 cttgtttata aagatcataa cccgccatat tcattaacca gttttgaaca attccgctga
     2821 aggcaccatc aataataatc atcacaacat ctaaatcgag gttatttgct acacaacctt
     2881 gttgctgaca cgcctgcaat acttcgcgga gagtctgcgg attaaagccc atcttttcgc
     2941 gtatcactcc ctcggccagc atcccatcat taaattcaca tttgtgatat aagattttca
     3001 gcaacgcctg ctggcgggga attttggcaa tatattgcaa gccgacaatc aatttttcac
     3061 gcaattgttg aaacgggtca tgctctaatc cagccgtcaa gtgttcctgg attaactccc
     3121 gcaatgaagg ctgttgcaac cacatctcat taaacagttg ggtcttgttc tcgaagtgcc
     3181 agtagatagc gccacgcgta acgttagcgg catcggcaat gtcgttgaac gtcgtcttgc
     3241 ttacgccatg ttgcgcaaac tgggcgatgg cagtttcaat cagttcttgc cgggtcttca
     3301 gagcttcggc tttggttctt tttgccatga ttaattattc aggaaataaa tatattcgac
     3361 acagggtgag aaaatagc
//
LOCUS       DAZPDU010000296         3322 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZPDU010000296 DAZPDU010000000
VERSION     DAZPDU010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.300"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..320)
                     /gene="fliZ"
                     /locus_tag="ACHURS_004411"
     CDS             complement(<1..320)
                     /gene="fliZ"
                     /locus_tag="ACHURS_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFR1239975.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDMLLDENGWQVEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTV"
     gene            complement(366..1085)
                     /gene="fliA"
                     /locus_tag="ACHURS_004412"
     CDS             complement(366..1085)
                     /gene="fliA"
                     /locus_tag="ACHURS_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFR1239976.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENQLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(1250..2824)
                     /locus_tag="ACHURS_004413"
     CDS             complement(1250..2824)
                     /locus_tag="ACHURS_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HFR1239977.1"
                     /translation="MAQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDD
                     AAGQAIANRFTANIKGLTQASRNANDGISVAQTTEGALSEINNNLQRIRELSVQATNG
                     TNSDSDLNSIQDEITQRLSEIDRVSNQTQFNGVKVLASDQTMKIQVGANDGETIEIAL
                     DKIDAKTLGLDNFSVALGKVPMSSAVALKSEAAPDLTKVNATDGSVGGAKAFGSNYKN
                     ADVETYFGTGNVQDTKDTTDATGTAGTKVYQVQVEGQTYFVGQDNNTNTNGFTLLKQN
                     STGYEKVQVGGKDVQLANFGGRVTAFVEDNGSATSVDLAAGKMGKALAYNDAPMSVYF
                     GGKNLDVHQVQDTQGNPVPNSFAAKTSDGTYIAVNVDAATGNTSVITDPNGKAVEWAV
                     KNDGSAQAIMREDDKVYTANITNKTATKGAELSASDLKALATTNPLSKLDEALAKVDK
                     LRSSLGAVQNRFDSAITNLGNTVNDLSSARSRIEDADYATEVSNMSRAQILQQAGTSV
                     LAQANQTTQNVLSLLR"
     gene            3079..3322
                     /locus_tag="ACHURS_004414"
                     /pseudo
     CDS             3079..3322
                     /locus_tag="ACHURS_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460913.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar cap protein FliD N-terminal
                     domain-containing protein"
BASE COUNT          852 a          789 c          712 g          969 t
ORIGIN      
        1 accgtacctg ggctcatttc agtttgctca aaaagaaatt gcttaaagcc gataatatcg
       61 aaaaaatcac tctgcgtttt gcagtgtaaa tcaccacaaa atcggcacaa tgccgcccat
      121 gatttttgtt ccacttgcca gccattttca tcaagcagca tgtccaggcg ggaaatctcg
      181 attttattca ctattttgcc gtcgcgaacg agggtaatgc ggtcgagtaa tttacggcaa
      241 tgcgcgcaat gagtctggct gtgtttaaag tctttaagat agcggcttaa tggccgtctt
      301 ttcaggtgct gcaccatcat taagaactcc tggtagtcaa aattaaagtg cggcatttac
      361 tgacgttata acttacccag tttagtgcgt aaccgtttaa tggcctggct gtgtaactga
      421 ctgacccgcg attccccgac ctccagcacc gcgccaatct ctttgagatt cagctcttcc
      481 tggtaataga gggttaatac cagtttttcg cgctccggca acgtttcgat ggcttccatc
      541 acccgctggc gcagattact gtccagcagt tgttgtagct ggttttctcg ctgatgatca
      601 tcagtaacca gttcgatgct atcgccgtgc tcttcgcgcc actcatcgta ggagaagagc
      661 tggctgttat tggtgtcgag caacatttgg cgataatcgg caatatcgat ccctaaacgt
      721 tccgctacct cagtttccgt ggcgttgcgg ccaagttcct gctccagttg ccctattgcc
      781 tgtgccactt cacgcgcgtt gcgtcgcacg ctgcgcggca cccagtcacg gctgcgaagt
      841 tcatccagca tagcgccacg gatacgctgc actgcgtaag ttgtaaatgc cgttccttgt
      901 agggcgtcat agcgttcgac ggcattaagt aacccaatgc cgcccgcctg tagcagatcg
      961 tcaagttcca cgctcgcggg cagtcgaacc tgcaggcgca atgcttcgtg acgcaccagc
     1021 gggacataac gctgccacag cgagtgttta tccattacac cttcagcggt atagagtgaa
     1081 ttcacgataa acagccctgc gttatatgag ttatcggcat gattatccgt ttctgcaggt
     1141 tttttaatcg gacgattagt gggtgaaatg aggggttatt taggggttac cggtaaattg
     1201 cggacagaaa aaaccccgcc ggtggcgggg tttgagcgat aagtgtaaat taacgcagca
     1261 gagacagtac gttctgcgtg gtctggttag cctgtgccag aacagaggta cccgcttgtt
     1321 gcaggatctg cgcacgagac atgttagaca cttcggttgc gtagtcagca tcttctatac
     1381 ggctacgggc agaagacagg tcgtttacgg tgttgccaag gttggtgatg gcagagtcga
     1441 aacggttttg tactgcaccc aaagaactgc gcaacttatc aacttttgcc aaagcttcgt
     1501 ctaatttgga taatggattt gtggttgcta aggctttcaa atctgaggca ctgagttcag
     1561 cacctttggt tgccgtctta ttcgtgatat tggctgtata aaccttatca tcttcacgca
     1621 taattgcctg tgcagaacca tcatttttta ctgcccattc aactgcctta ccattaggat
     1681 cagtaataac agacgtgtta cctgtagcgg catctacatt tactgcaatg taggtgccgt
     1741 ctgatgtttt agcagcaaat gaattaggta caggattccc ttgggtatct tgtacttggt
     1801 ggacatctag gttttttccc ccaaaataaa cagacattgg tgcatcatta taagctaatg
     1861 ctttacccat tttacccgca gctaaatcaa ctgatgtggc agaaccatta tcttcaacaa
     1921 atgcagttac acgaccacca aagtttgcta actgaacatc cttaccaccc acctgaactt
     1981 tttcataacc tgtagagttt tgtttcaata atgtaaaacc gttcgtgttg gtattattat
     2041 cttgaccaac aaaataagtc tgcccttcca cctgtacttg ataaactttt gttcctgcag
     2101 taccggtcgc atcagttgta tcctttgtat cttgtacatt accggtacca aaataagttt
     2161 caacatcagc atttttataa ttgctaccga atgctttagc acctcccaca ctaccatcag
     2221 ttgcatttac cttagttaag tcaggagcgg cttcgctctt aagtgcaacc gcagaggaca
     2281 ttggaacttt tcctagtgct acgctaaagt tatcaagccc caaggtttta gcatcaattt
     2341 tatcaagggc aatctcaatg gtttcaccat cgttcgcacc tacttgaatt ttcatagtct
     2401 gatcagaagc cagaactttt acaccattaa attgtgtctg gttagaaaca cgatcaattt
     2461 cactaaggcg ttgtgtaatt tcatcctgaa ttgaattcag gtcggagtca gagtttgtac
     2521 cattagtggc ctgtactgac aattcacgaa tacgctgtaa gttattgttg atttcagaaa
     2581 gcgcaccttc agtggtctgc gcaacagaaa taccatcatt cgcgttacgg gaagcctgag
     2641 tcagaccttt aatatttgct gtaaaacggt tagcaatcgc ctgacctgct gcgtcatctt
     2701 tagcactgtt aatacgcagg ccagaagaga gacgttcaat ggcggagctc agagaagact
     2761 gagatttgtt caggttattc tgggtcaaca gcgacaggct gtttgtatta atgacttgtg
     2821 ccatgatctt ttccttatca attacaactt gatgttattg ggctgttgcc cacggtttct
     2881 caccgtaccc cttgtatcgg cacctgaatt tcgaacttta gaaaatttta gtttctctcc
     2941 tgttttttct taacggctga aatatccgta atatacaact ctactctgcg catcatcttc
     3001 gtgaatcaac tattaaactt tgcgcacaaa ttgccgataa cccgcttatc tactgttttc
     3061 aatcaaaagg aattaggtat ggcttcattt acatctcttg gcgttggttc gaatctgccg
     3121 ctggatacat tgctaaacaa cctgaccatc gctgaaaaaa aacgcttaaa cccaattact
     3181 cagcaacaga gtgacaatac cgcacgtctg acagcctatg gcacattaaa aatgtgcatt
     3241 agaaaaattc cagactgcaa atactgcgtt aaataaagcc gatctgttca aaagcactac
     3301 cgttaccagt agcaatgaag ac
//
LOCUS       DAZPDU010000297         3277 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZPDU010000297 DAZPDU010000000
VERSION     DAZPDU010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.301"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..842)
                     /gene="adrA"
                     /locus_tag="ACHURS_004415"
                     /gene_synonym="dgcC"
     CDS             complement(<1..842)
                     /gene="adrA"
                     /locus_tag="ACHURS_004415"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFR1239978.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRG"
     gene            complement(944..1264)
                     /gene="psiF"
                     /locus_tag="ACHURS_004416"
     CDS             complement(944..1264)
                     /gene="psiF"
                     /locus_tag="ACHURS_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFR1239979.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(1383..2798)
                     /gene="phoA"
                     /locus_tag="ACHURS_004417"
     CDS             complement(1383..2798)
                     /gene="phoA"
                     /locus_tag="ACHURS_004417"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFR1239980.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTSDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKTIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(2899..3159)
                     /gene="iraP"
                     /locus_tag="ACHURS_004418"
     CDS             complement(2899..3159)
                     /gene="iraP"
                     /locus_tag="ACHURS_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFR1239981.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT          771 a          868 c          780 g          858 t
ORIGIN      
        1 ccgcgcaggg taatttgtaa ctgtcgggta agcgccacaa tcgcttcatc gcccacatca
       61 tgaccccagg tatcgttgat gctcttgaaa tggtcgatat cgataatcag taacgttgca
      121 tcgcgattat gccgccgaca gttatcaaat tcattgcgta acatagtttc ccaatgacgt
      181 cggttataca cgcccgtcat gccgtcgcga gtactcatga cctgcaacct gcgtttatgt
      241 tccgccagtt tggttgccgt ctggtagctg acccagccaa acagcagagg ataaacgaca
      301 ataatgggaa gggagagcca ccattccagc ggcgcactat tgaacgacac ggtaatgccc
      361 gtaagctcga gggtgacaag gcaggaaacc accatcaaca ccagacccgc gacaaacaga
      421 cgggggccgc ctgcccccat caaattcaga cacataatca tcaacatagc ggtggaaggc
      481 agcacgttta cgcccattac gcctacccac attcccgcta atactgcatc ggtttttaag
      541 ttgtaaattt cccggctaag cggatcgacg gccctgctcg ctatctgcca ggctaaatgc
      601 ggccagacga acgcccagcc gaccaacacc agccaccacc agcccggcgg cgggtgcgaa
      661 accagcgttg aagcaatcgg taagaacatg ccagccaggc caaccgcacg gggtagtctg
      721 acgcgacggg cgaagcgcag cccggaccgc tgatgttcgt tttgaggagt taaaggaggt
      781 tcctccccat gcgccgccgc ttttttgaaa aagttttcat cattcattat ttttgggaac
      841 attcagaaac aattttccca aattatagag acggggctaa aggcagaagt atgaaatttc
      901 gggtgagcca ctttcgtagc tcacccgtta gcactatcag gtatcaggcg gctttcttga
      961 ggcaggcact cataaactta ttacgatcat cacctttcag agattgttgc gttgcttgat
     1021 tattacattc gcgcatcttt tgctgctgtg gcgtcaaact tttttcacca ggcgcagact
     1081 tgctgttctt caggcaatca ctcatgtagg tcttacgagc atcccctttc aacgcctgcg
     1141 ccgtcgcctg ctgattacag gaggtcatac gctgttgttg tggggttaag gttctctcgg
     1201 cagcgccgac ggtggttaaa aaaaccagac caaaaagcaa ggtaaccagt aatgttattt
     1261 tcatagcacc atccctcttc atgttttaac catgagcgtt tgcgcccgtg atctgccatt
     1321 aagtctggtt gctaacagca aaaaaaccac ccggcagcga aaattcactg ccgggcgcgg
     1381 ttttatttca gccccagagc ggctttcatg gtgtagaaga gatcggtctg gtcggtcagt
     1441 ccaacaacat tggcggcatg cggaccatac gccgcaatac gcaactgact gccggtatgt
     1501 tcttgtgaat cctcttcgga attcccgtaa ctcatcacca tcactgcgcc atctttagta
     1561 tttagcgcct gagtgaggcc cggcgctttg gtatccggcg caacaacctg gctggcgtgg
     1621 gcgtgatcag cggtgactat gaccagcgtg ttaccctcct ttttagcgaa ttccagcgcc
     1681 cgttgtacgg cttcatcgag atcgaccgtc tcgccaattt gcccacaagg attcgcagcg
     1741 tgatcctgtt tatcgattga cgcaccttca acttgcagga aaaatccttt ctcattttta
     1801 ctcaacaatt caatggtttt gtcggtcatc tgtgccaggg ttggtacact gtcattacgt
     1861 tgcggattag gcgtacaggt gactgcgggc ttatcgatat tgccgtggta cgttgctttc
     1921 ggccctagcc agcgcactgg catattgccg tcagcaaaca ggccaagcag gggtttttgc
     1981 tgattcgctt ccgtcaccga attcagtgag gcagcatcgt tcaccaactg ataaccacgc
     2041 gcctgtgcct gttcacgcag cgtttttccc tgccattcac cagcggttgc cgtttcagca
     2101 aaggtttttg cgccgccgcc aagcgtaacg tcggcacgag cgttaagcag ctgttcggta
     2161 atcgatcctt ttccgccttt ttccagagcg ttacccggac atttttcact ggtcgcgctc
     2221 ggaccgtagc atttgcgcga agtcacatgt gccaccagcg cagcgggcgt ggcatcctgc
     2281 aactctgcgg tagaaacgtt accggtcgcc agacctgcgg cttttgccat ttccagaatc
     2341 gttgggtgat ctttttcgtg aatatcgacg cccagcgcgc cgttataggt tttgacaccg
     2401 gttgaccagg cggttgctga tgcagccgag tcggtgacgt agtccggttt gccagttttt
     2461 ttattcagcg catagtgagt gtattgcccg gtaagcggta aggcatcgat acctttaaaa
     2521 aagccgcccg caccttcggc ataattacgt gcggcagtaa tttccgaatc ccccatccca
     2581 tcgccaatca gcaaaataat attttttgca ggtttatcgc taagagaatc acgcagagcg
     2641 gcggtctgat cactcgttaa gcggcgagca ccgccgggtg cagtaatatc gccctgagca
     2701 gcccggtttt ccagaacagg catttctggt gtccgggctt ttgtcacagg ggtaaacagt
     2761 aacggtaaga gtgccagtgc aatagtgctt tgtttcactt tattttctcc atgtacaaat
     2821 acattaaaaa ataaaaacaa agcgactata agtctcggcc gtgacaactt tatgacagct
     2881 gttgaaaaga ttaacttttt actgacgagg atgcttcaat aacttcttta cgtaatcgcg
     2941 cagcagctcc gtatcgtcgt caggaatgct ggcatcgggc tttacctcgt acagcgcccc
     3001 ctctacctga tcaatcaacc gctgttggtc attttgcgcc atattgcgaa gcattgcagt
     3061 gacgataatc tccaaagctt ctacctgcgc acacagttct ttcgactctt cttctttttg
     3121 ggcaagctta aataacaact cagcaatgag atttttcatg tctgtatttc cttatccaaa
     3181 gtatggagaa gttaacatta tgtttcacaa cttcatagtg tctggaagta ttatttttat
     3241 gacaaaggaa atactttgcg caaatatttt ttgtttg
//
LOCUS       DAZPDU010000298         3240 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZPDU010000298 DAZPDU010000000
VERSION     DAZPDU010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.302"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..406
                     /locus_tag="ACHURS_004419"
     CDS             <1..406
                     /locus_tag="ACHURS_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1239982.1"
                     /translation="DDIKEDDFLSLDELNDFLSMNESDLEVSSKKTSKQDREITDVSV
                     DSDHKNTQCPATDDELDPFDDEEDEREKAIKRAEKKKRFELARQKNKVSGLDPFDDSE
                     DDYYLSPKKKQTQDDKKQKIKPKMYFAGGGIK"
     gene            406..696
                     /locus_tag="ACHURS_004420"
     CDS             406..696
                     /locus_tag="ACHURS_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000666098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239983.1"
                     /translation="MKEYHGEKRYKDYLLKRYSISREGHLMKDTHGEVYRIRPKKEGR
                     GYFFYDGVTDLKIDALRFAVLYHFDIWNSIHQLRLKDGDPSNLKATNIITKR"
     gene            1017..1307
                     /locus_tag="ACHURS_004421"
     CDS             1017..1307
                     /locus_tag="ACHURS_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239984.1"
                     /translation="MRHTVTRLLPEAYSRLAAIKDKYPAMSHTALVGEILKEHTALTP
                     FDAIKGVAEGSKAIRITTGQLELLKAQSAVAGCSMIQVLSAKVHQLADKLGV"
     gene            1307..1726
                     /locus_tag="ACHURS_004422"
     CDS             1307..1726
                     /locus_tag="ACHURS_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239985.1"
                     /translation="MLCTFDYHAFYRHRGHLVRENNGTWKDLTRNAEPFTHQQLKSRF
                     SRWKNAPADTGDFEIIHTFDYASEDDLHDAIKLIEDFNTSEDVDGDKYKSRGNRYNRD
                     DITQAINAGQSQRYITEKYGISRRQYFYVKSLINKDN"
     gene            1894..2217
                     /locus_tag="ACHURS_004423"
     CDS             1894..2217
                     /locus_tag="ACHURS_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239986.1"
                     /translation="MNNYPMQIMVDNDTAMMVQSFIDAGIAINFDKLLKLMADNAENI
                     SDFIQSVEFNEPRMMLPITDSNMKRLVIEQTNIYSISPEQFLKGAVTILYSDNILVTD
                     SVRVH"
     gene            2647..>3240
                     /locus_tag="ACHURS_004424"
     CDS             2647..>3240
                     /locus_tag="ACHURS_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239987.1"
                     /translation="MIRNEHEMLIEHRPSALDIHELTLRESFDRAWRESHEVNKGPYI
                     PPEPLEIPRVEIDFSMNERCELDLKLKLQRRYFQAIKDSQEAMRSVYEKIQSKLEDEI
                     DEMVISMELQTNSYACFSRLDRDGWGYDPVEIGKVIADLPEGQRVVRTERAVRAPHRL
                     SLIVADKSQAELEEIAKEKLMKARENEINLLKSRLAKL"
BASE COUNT         1074 a          579 c          678 g          909 t
ORIGIN      
        1 tgatgatatt aaagaagatg actttctttc tttggatgaa ttaaatgatt ttctctctat
       61 gaatgaatct gaccttgaag taagctctaa aaagacttct aaacaagaca gagaaatcac
      121 tgatgtgagt gttgatagtg accataaaaa cacacagtgc cccgccactg atgatgagtt
      181 ggaccccttc gatgatgaag aggatgaacg tgaaaaagcc attaagaggg cagagaagaa
      241 aaagagattc gagcttgcaa gacaaaagaa caaagtatcg ggtttagacc cattcgatga
      301 cagtgaagat gattattatc tttcaccgaa aaagaaacaa actcaggatg acaagaagca
      361 aaaaattaaa ccgaagatgt attttgccgg tggaggaatt aagtaatgaa agaatatcac
      421 ggtgaaaaga gatacaaaga ttacctgtta aagcgttaca gcatttctcg tgaaggtcat
      481 cttatgaagg atactcacgg ggaagtttac cggattagac caaagaaaga agggaggggt
      541 tatttctttt acgatggtgt gacagattta aagattgacg ctttgaggtt tgcagtgctg
      601 tatcattttg atatctggaa ttcaatccac cagctacgat taaaagacgg tgaccccagc
      661 aacttaaagg ccaccaatat tatcacaaaa cgttagtttg ttttaccatc atcaatcgta
      721 attgtgattt catatttgaa aagcttaatg ctgaaattga atcgtgtact tctggcgata
      781 atcattttta aaagctgata cacaagaagc accagaaaca caaagcaacc taagcttgcg
      841 aatctgtgag attcagcaat tgtttcaaga aagcccatta attcccctta tttatcaaag
      901 ggaatgacct gtatcagccc cctacaacca tattaacatg taaatgagtt gttgacaata
      961 tgttataaat atgttaaagt tagttataag tcaaagattt taagaaggaa aatttaatga
     1021 gacacacagt tacacgcctt ttgccagagg catattccag actggcagca atcaaagaca
     1081 aatacccagc catgagccac acagcacttg ttggtgagat tctgaaggaa cacacagccc
     1141 ttacaccgtt tgacgctatc aagggcgttg cagagggttc taaggccatc agaatcacaa
     1201 cgggccaact ggaactactc aaagcacagt ccgcagtggc aggttgctca atgattcagg
     1261 tgttgagtgc caaggttcat caactggcgg ataagctggg ggtttaatgc tatgcacatt
     1321 tgattatcat gcgttttatc gccatagagg tcaccttgtc cgtgagaata acggaacatg
     1381 gaaagattta actcgtaatg ctgaaccctt cacacaccag caattaaaat cccgcttctc
     1441 cagatggaag aatgcaccag cagatactgg tgattttgag attatccata ccttcgatta
     1501 tgcctcagaa gatgaccttc acgatgcaat taagctgatt gaagacttca atacctcaga
     1561 agatgtggat ggtgacaaat ataaatcccg tggtaaccgc tataaccgtg atgatatcac
     1621 tcaggccata aatgctggtc aatctcagcg atacattaca gaaaaatatg gtatttcacg
     1681 gagacagtat ttctacgtta agtcattgat taataaagac aattaaaaat aaccatctaa
     1741 gaaacggtag aatgcacttt acaggcagag ttgcacctta atttgataaa ttaccttacc
     1801 agccagtgca actccctctg taacccgcat ggttactggc ctcagaatgg ctattttaag
     1861 aggtctgagg tgtgtttcat aggagattta aaaatgaata attacccaat gcaaattatg
     1921 gttgataacg acactgctat gatggtgcaa agcttcattg atgcaggtat cgcaattaat
     1981 tttgataaat tactaaagct gatggctgat aatgcagaaa acatctctga ctttattcag
     2041 tcggtggagt ttaacgagcc tcgaatgatg ctaccaatta cagacagcaa catgaaaaga
     2101 ttggttatcg agcaaacgaa catatacagt atttccccag agcaattttt gaaaggtgct
     2161 gtgacaatcc tgtacagtga taatattctg gtgacggatt ctgtgagggt tcactaatgg
     2221 gtttaataat tgcttggacg ttgcttggct tatttgttgg ttgcattatt aacaatgcgg
     2281 taaataagaa ttgatgttat caacctgtta aaaagctgtt gacaaataac cttaagcatg
     2341 gcataatttt aattatggga gagcaattct ctcttatttt tctttcaata atccgatgga
     2401 tggatattct gagttggaaa ggtgcaatgc ttttcgtcat ggcctttcag gttagtctga
     2461 gaggctatct caaaaagtac tcttgttcat tcattattga atcctttgcc cttcacagaa
     2521 atgtttaggg ctttttcagt ttttaaaagc acacttggca ggttcttgtt cagtcagact
     2581 cttcacctct gattgctgag tgtgcttcta taaattgaaa taacaaataa actaaggggt
     2641 gaacgaatga ttagaaatga acatgaaatg cttatcgaac acagaccaag tgcacttgat
     2701 attcacgaat taactttgcg tgaatctttt gatagagctt ggcgggaatc acacgaagta
     2761 aataaaggtc catatattcc accagaacca ctcgagattc caagagttga aattgatttt
     2821 tctatgaacg aacggtgtga acttgatttg aagttgaagc tacaacgccg atatttccaa
     2881 gctattaaag actctcaaga agcaatgaga agtgtttacg agaaaattca gtccaagctt
     2941 gaagacgaaa ttgacgaaat ggttatttcg atggagcttc aaaccaattc ttatgcttgt
     3001 ttctctcgac tagaccgtga cggttggggg tatgaccctg tagagattgg taaagtgatt
     3061 gcagatttac cagaaggtca aagagttgtt cgtacagaaa gagctgtcag agcaccacat
     3121 cgactgtctc taattgttgc tgataaatca caagctgagc ttgaagaaat tgccaaagag
     3181 aaactgatga aagcaagaga aaatgagatt aatttgctga aatcaagact ggctaagctg
//
LOCUS       DAZPDU010000299         3217 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZPDU010000299 DAZPDU010000000
VERSION     DAZPDU010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.303"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..496
                     /locus_tag="ACHURS_004425"
     CDS             <1..496
                     /locus_tag="ACHURS_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR1239988.1"
                     /translation="SRAPFIITPPLFRIEAGDDSSLRIIKTADNLPENKESLFYINVR
                     AIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVNDAWTSLEFKRSDHSLNIYNP
                     TEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAINDYGGSSGTET
                     RPLQ"
     gene            526..3042
                     /locus_tag="ACHURS_004426"
     CDS             526..3042
                     /locus_tag="ACHURS_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR1239989.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQTSSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWNYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNDKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLNGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHTHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            3053..>3217
                     /locus_tag="ACHURS_004427"
     CDS             3053..>3217
                     /locus_tag="ACHURS_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1239990.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGRE"
BASE COUNT          939 a          771 c          731 g          776 t
ORIGIN      
        1 aagccgtgcc ccatttatta taaccccacc gctatttcgt attgaagctg gcgatgactc
       61 atcactgcga attattaaaa cagctgataa cctgcctgaa aataaagagt cgctgttcta
      121 cattaatgtt cgtgccattc cagcaaagaa aaaatcagat gatgttaatg ctaacgagtt
      181 gacgctggta tttaaaacac ggatcaaaat gttttatcgc cccgcacacc tgaagggacg
      241 ggtaaacgat gcgtggacat cactggaatt taaacgtagt gaccattcac tcaatatata
      301 taacccaact gaatattacg tcgtatttgc cggactggca gtcgataaaa ccgatctcac
      361 aagcaaaatt gaatatatcg cgcccggaga acataaacag ttaccacttc ctgcatctgg
      421 cggaaagaac gtgaaatggg ctgcaatcaa tgattatggc ggcagttccg ggacagaaac
      481 tcgtccactg caataacaaa tataaaaaac acaggtcatc agggaatgcc acaacgacac
      541 caccagggac ataaacgcac accgaaacag ttggcgctca tcatcaaacg ttgtttgccg
      601 atggtgctca ctggcagcgg catgctttgc actaccgcta acgccgaaga gtattatttc
      661 gaccccatta tgctggaaac tacaaaaagt ggtatgcaaa caaccgatct gtcacgtttt
      721 tcaaaaaaat acgcacaact accaggaact tatcaggttg atatctggct gaataaaaag
      781 aaggtttcac agaaaaaaat tacatttacc gccaatgcag agcaacttct gcagccacag
      841 tttacggtag aacaactacg tgagctgggt attaaggtgg atgaaatccc ggcgctggct
      901 gaaaaagatg acgatagcgt gatcaactcg cttgaacaaa tcattcccgg tacagcagct
      961 gaatttgatt tcaatcatca gcgacttaat ttgagcattc cccaaattgc actgtaccgt
     1021 gatgcaagag gttacgtctc cccttctcgt tgggacgatg gtataccaac gctgtttacc
     1081 aactactcgt ttacaggttc tgataaccgt taccgccagg gcaatcgtag ccaacgacag
     1141 tacctgaata tgcaaaatgg tgctaatttt ggcccctggc gattacgcaa ctattccaca
     1201 tggacacgca acgatcagac atcaagctgg aataccatca gtagttattt acaacgtgat
     1261 atcaaggcat tgaagtctca gttgcttctg ggagaaagcg ccaccagcgg cagtattttt
     1321 tccagctaca cctttactgg cgttcaactc gcttccgacg ataatatgtt gccaaacagc
     1381 cagcgcggat ttgccccaac ggtacgcggt atcgcaaaca gtagtgcaat cgtgactatc
     1441 aggcaaaatg gttatgtgat ctatcaaagc aacgtgccag cgggtgcctt tgaaattaac
     1501 gatctctacc cctcttccaa cagcggcgat ttagaagtca cgattgaaga aagtgacggt
     1561 acgcaacgtc gctttatcca gccttattct tcattaccca tgatgcagcg acctgggcat
     1621 ctaaaatata gcgcgaccgc tggacgctat cgcgctgatg caaacagtga tagcaaagaa
     1681 cccgaatttg ctgaagccac ggcaatatat ggtttgaata atacttttac gctgtatggt
     1741 ggcctgctcg gttctgaaga ttattatgcg ctggggatcg gtatcggcgg cacacttggc
     1801 gcactgggcg cgttgtcgat ggatatcaac agagctgaca cccaattcga taaccagcac
     1861 tcttttcatg gctatcaatg gcgtacgcag tacatcaaag atatcccgga aaccaacacc
     1921 aatatcgctg tcagctacta tcgctatacc aacgatggct attttagttt tgatgaagcc
     1981 aatacccgta attggaacta taacagtcgc caaaaaagtg aaattcaatt caacatcagc
     2041 cagacaatat ttgatggggt aagtctgtat gcctccggtt cgcagcaaga ctattggggc
     2101 aataacgata aaaacaggaa tatctcagtt ggggtttccg gtcagcaatg gggaattggt
     2161 tacagcctga attatcaata cagtcgctac acggatcaaa ataatgaccg cgcactctct
     2221 ttgaatctca gtattccgtt agaacgctgg ttaccgcgta gccgggtttc ctatcagatg
     2281 accagccaga aagatcgccc aacccaacat gaaatgcgtc ttaatggctc actgctggat
     2341 gatggtcgcc tgagctatag cctggaacaa agtctggatg acgataacaa ccataacagt
     2401 agcctgaacg ccagttaccg ttcaccttat ggcaccttca gtgccggata cagctacggt
     2461 aatgacagca gccaatacaa ttacggcgtt accggcggcg tggttatcca tactcatggc
     2521 gtgacgctct cgcaatatct gggcaacgct tttgcgctta tcgatgctaa tggggcttct
     2581 ggcgtgagga tacaaaacta tccggggatt gctactgatc cctttggcta tgcagtggtt
     2641 ccttatctca cgacttacca ggaaaaccgt ctctcggtag atactacgca gctgcccgat
     2701 aacgtcgatc ttgagcaaac aacacagttt gtggtgccca acagaggtgc aatggtagcg
     2761 gcgcgtttca acgccaatat cggttatcgc gtacttgtta cagtcagcga tcgcaacggt
     2821 aaaccgttgc cctttggcgc tcttgccagc aacgatgaga cggggcaaca aagtatcgtc
     2881 gatgagggcg gcatactata tctctctggg atatcgagta aatcacaaag ctggactgta
     2941 cgctggggaa atcaggcaga tcaacaatgt cagtttgctt ttagtacacc ggattcagaa
     3001 ccaacaacct ctgtattaca aggcacggcg cagtgccatt aaggataaaa aaatgaaaag
     3061 agcgcctctt ataacaggac ttttgttgat atccacatcc tgcgcttatg cctcctcagg
     3121 agggtgtgga gctgacagca ctagcggtgc gacaaattac agcagtgtgg ttgatgatgt
     3181 tacggtgaac cagacagata acgtgacagg acgggag
//
LOCUS       DAZPDU010000300         3198 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZPDU010000300 DAZPDU010000000
VERSION     DAZPDU010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.304"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            145..1827
                     /locus_tag="ACHURS_004428"
     CDS             145..1827
                     /locus_tag="ACHURS_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFR1239991.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGAPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1879..3036
                     /locus_tag="ACHURS_004429"
     CDS             1879..3036
                     /locus_tag="ACHURS_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFR1239992.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(3002..3124)
                     /locus_tag="ACHURS_004430"
     CDS             complement(3002..3124)
                     /locus_tag="ACHURS_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1239993.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESIINYSPFVLQHP"
BASE COUNT          969 a          686 c          636 g          907 t
ORIGIN      
        1 agtaatgtca acgctttatg gactgttatt tccctttttt tagctaaatc tgctatctct
       61 ttatgtgact aacttcactt acatccactt atttctcttc gtaaaattac ttcggaatta
      121 agtacaataa gaagaggaac atttatgaag tctgcattaa agaaaagtgt cgtaagtacc
      181 tcgatatctt tgatactggc atctggtatg gctgcatttg ctgctcatgc ggcagatgat
      241 gtaaagctga aagcaaccaa aacaaacgtt gctttctcag attttacgcc gactgaatac
      301 agtaccaaag gaaagccaaa tattatcgta ctaaccatgg atgatcttgg ttatggacaa
      361 cttccttttg ataagggatc ttttgaccca aaaacaatgg aaaatcgtga agttgtcgat
      421 acctacaaaa tagggataga taaagccatt gaagctgcac aaaaatcaac gccgacgctc
      481 ctttcattaa tggatgaagg cgtacgtttt actaacggct atgtggcaca cggtgtttcc
      541 ggcccctccc gtgccgcaat aatgaccggt cgagctcccg cccgctttgg tgtctattcc
      601 aataccgatg ctcaggatgg tattccgcta acagaaactt tcttgcctga attattccag
      661 aatcatggtt attacactgc agcagtaggt aaatggcact tgtcaaaaat cagtaatgtg
      721 ccggtaccgg aagataaaca aacgcgtgac tatcatgaca acttcaccac attttctgcg
      781 gaagaatggc aacctcaaaa ccgtggcttt gattacttta tgggattcca cgctgcagga
      841 acggcatatt acaactcccc ttcactgttc aaaaatcgtg aacgtgtccc cgcaaaaggt
      901 tatatcagcg atcagttaac cgatgaggca attggcgttg ttgatcgtgc caaaacactt
      961 gaccagcctt ttatgcttta cctggcttat aatgctccgc acctaccaaa tgataatcct
     1021 gcaccggatc aatatcagaa gcaatttaat accggtagtc aaacagcaga taactactac
     1081 gcttccgttt attctgttga tcagggtgta aaacgcattc tcgaacaact gaagaaaaac
     1141 ggacagtatg acaatacaat tattctcttt acctccgata atggtgcggt tatcgatggt
     1201 cctctgccgc tgaacggggc gcaaaaaggc tataagagtc agacctatcc tggcggtact
     1261 cacaccccaa tgtttatgtg gtggaaagga aaacttcaac ccggtaatta tgacaagctg
     1321 atttccgcaa tggatttcta cccaacagct cttgatgcag ccgatatcag cattccaaaa
     1381 gaccttaagc tggatggtgt ttccttgctg ccctggttgc aagataagaa acaaggcgcg
     1441 ccacataaaa atctgacctg gataacctct tattctcact ggtttgatga ggaaaatatt
     1501 ccattctggg ataattacca caaatttgtt cgccatcagt cagacgatta cccgcataac
     1561 cccaacactg aggacttaag ccaattctct tatacggtga gaaataacga ttattcgctt
     1621 gtctatacag tagaaaacaa tcagttaggt ctctacaaac tgacggatct acagcaaaaa
     1681 gataaccttg ccgccgccaa tccgcaggtc gttaaagaga tgcaaggcgt ggtaagagag
     1741 tttatcgaca gcagccagcc accgcttagc gaggtaaatc aggagaagtt taacaatatc
     1801 aagaaagcac taagcgaagc gaaataacta aaccttcatg cggcggattt ttccgccgcc
     1861 ttattgagcg agatagcgat gcacgttaca gccaagccct ccagttttca atgtaatctc
     1921 aaatgtgatt actgttttta ccttgaaaaa gagtcgcagt ttactcatga aaaatggatg
     1981 gatgacagca ctctgaaaga gttcatcaaa caatatatcg aagcgtctgg caatcaggtc
     2041 tattttacct ggcaaggcgg tgaacccact ctggctggcc tggatttttt ccgtaaagtt
     2101 attcactatc aacaacgcta tgcaggccaa aaacgtattt ttaatgcatt acaaacgaat
     2161 ggcattttat tgaataatga atggtgtgcc tttctcaaag aacatgaatt tctggttggt
     2221 atctcgatcg atggccccca ggagttacat gaccgttaca gacgcagtaa ttcaggtaac
     2281 ggtacttttg caaaagtgat agcagccatc gagcgtctga aatcatatca agtagagttt
     2341 aatacgttaa ccgtcattaa taacgttaat gtccattacc ctcttgaggt ttatcatttt
     2401 ttaaaatcta tcggcagtaa acatatgcaa tttatcgaat tgctagaaac cgggacgccg
     2461 aatattgatt tcagtggtca tagtgagaac acattccgta tcattgattt ttctgtgcct
     2521 cccacggctt atggcaagtt tatgtcaacc atttttatgc aatgggttaa aaacgatgtg
     2581 ggtgaaattt tcattcgtca gtttgaaagc tttgtcagcc gttttttggg gaatgggcat
     2641 acaagttgta ttttccagga gtcctgcaag gataatctgg ttgttgaaag taatggagac
     2701 atttacgaat gcgaccattt tgtctatcca cagtacaaaa ttggaaacat taataaatct
     2761 gaactcaaaa cgatgaacag tgtacaactg acagcgcaaa aaaaacggat ttcagcgaaa
     2821 tgtcagcaat gtgcatataa acctatctgc aatggcggtt gtcctaagca tcgtattact
     2881 aaagtaaaca atgagactgt ttcttatttt tgcgaaggtt ataaaatcct tttttcaacc
     2941 atggtacctt atatgaacgc catggttgag ttagctaaga acagagtacc gctttaccac
     3001 attatggatg ttgcaaaaca aatggagaat aattaataat gctctctttt aactccattc
     3061 ttagcctcct tcttatgacc ttaacaaggc acccgacata aattcctgtc gggtgtttcg
     3121 tcatcatcat cttcccttgc gaaaatcctg gcttacgtgg tatgcgtcag ggcattagca
     3181 atacccttga ttatcaat
//
LOCUS       DAZPDU010000301         3185 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZPDU010000301 DAZPDU010000000
VERSION     DAZPDU010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.305"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(9..635)
                     /gene="dsbA"
                     /locus_tag="ACHURS_004431"
     CDS             complement(9..635)
                     /gene="dsbA"
                     /locus_tag="ACHURS_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFR1239994.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(652..1638)
                     /gene="srkA"
                     /locus_tag="ACHURS_004432"
     CDS             complement(652..1638)
                     /gene="srkA"
                     /locus_tag="ACHURS_004432"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFR1239995.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQTEAVGRYLGRMHQTGHKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFGEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(1715..1984)
                     /locus_tag="ACHURS_004433"
     CDS             complement(1715..1984)
                     /locus_tag="ACHURS_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFR1239996.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            2054..2638
                     /gene="mobA"
                     /locus_tag="ACHURS_004434"
     CDS             2054..2638
                     /gene="mobA"
                     /locus_tag="ACHURS_004434"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFR1239997.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHVLASRLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            2620..3147
                     /gene="mobB"
                     /locus_tag="ACHURS_004435"
     CDS             2620..3147
                     /gene="mobB"
                     /locus_tag="ACHURS_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFR1239998.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTEKPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT          832 a          772 c          769 g          812 t
ORIGIN      
        1 acattgaatt attttttctc ggacagatat ttcactgtat cagcatactg ctgaacaaaa
       61 acatccatat tgctggtatc cataccctgc ggattcagct gatatttacc gttaacaaac
      121 atcgccggaa cgccacgcaa ttgcacgtca gctgcagctt tttcctgctg agcgaccaga
      181 gatttgacca cgaagctgtt ccacgccgcg tcgtactctt cacctttaat acctgcgttg
      241 ataaatacat cgcggatatc agatgctgaa cgaatggtct gggttttctg tacgccttca
      301 aacagcggaa cagtgacttt gtcttccacg cccagcgcca tcgccacagc ccatgcctga
      361 gtcagatctt tgcccaggtc accacccatg aagttgacgt ggtatttagt catcttcacg
      421 ccttccggca gttttttctt cacattatca gaaatatgca gaacttcttc aaactgatag
      481 cagtgcggac agaagaaaga gaaaaactcc agcacttgcg gcgcgccagc taccggtttt
      541 tccagggtag tgtactgttt accatcttca tactgcgccg ccgatgcgct aaacgctaaa
      601 actaaaccag ccagcgccag ccaaatcttt ttcatgatct actctctccg attaatacat
      661 aggtgttaat tgtaaagggg gttcttgtag aacttttgcc tgttctataa aagtcgccgt
      721 ttgtcgcagc cagtaatctt ccccggttaa ccacgggaaa tttttcggga acgcgggatc
      781 agcccaacgc cgcattagcc aggcaagata ataaaccaaa cgcatggcgc gtaaaggttc
      841 aatcagtccg atttcagcgg tgtcgaactc gccaaattct tcataagctt caataatagt
      901 ttccagttgc atccgctgct cggctttatc gccattgagc aacatccaca aatcctgaat
      961 agctggacca ttacgtgcat catccagatc aacaaacatt ggaccatcgc gccagagaat
     1021 attcccggcg tggcagtctc catgtagccg cagaacggtg aaatcttccc gccagtgtgc
     1081 tgtaacggcg gcgatcagct catctgtcgc tttcaggaat gccgctttca acccggaagg
     1141 tatcagtgta gcgtcctcaa acagcttgcg tggctcaatg agatattcat tcagaccgat
     1201 ggtcgggcga tggataaaaa gctgtttgtg ccccgtctgg tgcatacgcc ctaaataacg
     1261 cccaaccgct tccgtctgat cgatattatc agcttcgaac tggcgaccac cgacgcttgg
     1321 aaaaacagcg aaataaaatc cctgatgatt caataaagtc tgaccgttaa aggccacagg
     1381 tgctgcgacc ggaacttcat catttaccag ctgcaacgca aattgatgtt cttcgaggat
     1441 ttgatcggct gtccaacgtt cagggcgata aaatttgacg acaaaacgtc gacgctcttc
     1501 gtcctgaaat tgatagacac ggttttcata gctgttaagc ggggtaagac cggaatccac
     1561 ccggatccct tgctcaaaca gagcgtccat gatggtatcc gggtgtagtg tctggaaagt
     1621 aaaagcgctg ttgttcatcc catcatccgg aaaacattac gaatgatgaa ggatatcatt
     1681 tcacgatgat tttggtggcg ccgcttacaa gcttttactc tttaattacg ccgcgagcgc
     1741 gtaacagcgc cgttttgaag tcttcttcat aatctttctg caaacccggg atcaccgcat
     1801 ctttcgcgga atcacgcatt ttcaggtgat agatcagaat gtcatccgtc aaatccgcca
     1861 gttcgccgtc aaaacctgac tctttcgcca gtttctgcaa aaattgcaac aggttaaggt
     1921 ctggctcttt ttgccaggct ggctggagga gttcaataac ttcattcaga cgtttacatt
     1981 tcatagtgat gctccttact cttgagacag acacgttagc agggtcaatc ccacaataaa
     2041 agaggcgata tcggtgaatc tgatgacgac gataacaggc gttgtgctgg caggcggtaa
     2101 agccagacga atgggcggcg tagataaagg attgcttgaa ttaaacggca aaccattatg
     2161 gcaacatgtc gctgacgcgc ttatgacgca gctctctcac gtcgtggtta atgccaatcg
     2221 tcatcaggaa atctatcagg caagcggtct gaaagtgatt gaagattcac tggcggatta
     2281 cccaggccct ctggcaggaa tgctttcagt aatgcagcag gaagctggtg agtggttttt
     2341 gttttgtccg tgcgatacgc cttacattcc ccatgtttta gcttcccggc ttaatcatca
     2401 gcgcaaagat gcgcctgtcg tgtgggtcca tgacggtgaa cgcgatcacc cgactattgc
     2461 tctggtaaac cgtgctattg agcctttatt actagaatat ctgcaagcag gagaacgccg
     2521 ggtaatggca tttatgcgtc tggctggcgg ccatgcggtt gatttcagcg atcataaaga
     2581 tgcatttgtt aacgtgaata cgccagagga gcttgcccga tggcaggaaa aacgatgata
     2641 ccgttactcg cctttgccgc gtggagtggt accggaaaaa ctacgctttt gaaaaaactg
     2701 atcccggcat tatgcgccag agggatccgt ccagggctga ttaagcatac gcaccatgat
     2761 atggatgttg ataagccagg caaagatagc tatgagctgc gcaaggctgg cgcggcacaa
     2821 accatcgttg ccagccagca acgatgggcc ttgatgacgg aaaaaccaga cgaagaagag
     2881 ctggatctac agtttctcgc aagtcgaatg gatacctcaa agctggattt gattctggtc
     2941 gaagggttta agcatgaaga gatcgcaaag attgtgctgt ttcgcgatgg agccggacat
     3001 cgacctgaag aattagtgat agacaggcat gttattgctg tagccagtga tgtgccgctt
     3061 aatcttgatg tcgcattact ggatattaat gatgttgagg ggctggctga ttttgtggtg
     3121 gagtggatgc aaaagcagaa cggataagca agagtaggct ggataagacg cgccagtgtc
     3181 gcatc
//
LOCUS       DAZPDU010000302         3154 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZPDU010000302 DAZPDU010000000
VERSION     DAZPDU010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.306"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..122
                     /locus_tag="ACHURS_004436"
     CDS             <1..122
                     /locus_tag="ACHURS_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1239999.1"
                     /translation="GTVNKSKVPLGLSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            456..2735
                     /gene="ydeP"
                     /locus_tag="ACHURS_004437"
     CDS             456..2735
                     /gene="ydeP"
                     /locus_tag="ACHURS_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFR1240000.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAADRTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPHS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2983..>3154
                     /gene="safA"
                     /locus_tag="ACHURS_004438"
     CDS             2983..>3154
                     /gene="safA"
                     /locus_tag="ACHURS_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFR1240001.1"
                     /translation="MYATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTH"
BASE COUNT          864 a          713 c          746 g          831 t
ORIGIN      
        1 tggggacagt aaataaaagc aaagtacctc tgggtttaag cgcaacctat ggacaaacag
       61 gaaacaaggt ttcagccgga accgtccagt cagtcattgg tgtcactttt atctatgaat
      121 aattgtgatt ttgaccttag tctatgcgct ccattcacga aaattgcgca gataattatt
      181 ttgtgaaggc tattatccta cacctgtaag aaaatccgcg cacaattgaa gccagagtct
      241 tattttttat attgaaaaat atgttaattt aaacactatg agagatgatg cttaacttat
      301 cgtatttaat ctattgttta acgataatag attaaaaata gaaagagagt agtcccctac
      361 tcttatatat ccatgttggc gataatccct ttgtatgtac tgtgcatcat cgctattaca
      421 aatcctaata attcatttcc acacaggata agtagatgaa gaaaaaaatt gaatcctacc
      481 agggtgctgc aggtggttgg ggtgctgtta aatccgtagc gaatgcagta cgtaagcaga
      541 tggatatacg ccatgatgtt attgccatgt ttgacatgaa taagccagag ggctttgact
      601 gtccgggttg tgcatggcca gatcctaagc acagtgcgtc attcgacatt tgtgaaaacg
      661 gcgcaaaagc aatcgcctgg gaagtcacgg ataagcaggt aaatgcctct ttctttgctg
      721 agaatacggt tcaatcatta cttacctggg gagatcacga gcttgaggct gcagggcgtc
      781 tcactcagcc tttgaaatat gatgccgtca gcgactgtta caagccatta agctggcaac
      841 aagctttcga cgaaattggc gcacgccttc aaagctatag tgatcccaat caggttgaat
      901 tctatacttc gggccgcact tccaatgaag ctgcctttct ttatcagctt tttgcccgtg
      961 aatacgggag caataacttt cccgactgct ccaacatgtg ccatgaaccg acaagcgtgg
     1021 gtttggcagc gagtatcggt gtaggtaaag ggaccgtgtt gctggaagac tttgagaagt
     1081 gcgatttagt catttgcatt gggcataacc ctggtacaaa ccaccctcgc atgctgactt
     1141 cgttgcgcgc tttagtgaaa cggggagcga aaatgatcgc catcaatcct ctacaggaac
     1201 gtggcctgga gcgatttacc gcaccgcaaa acccgtttga aatgctgacg aactctgaga
     1261 ctcagttggc cagtgcctac tacaacgtgc gcattggtgg cgatatggcg ttgctcaagg
     1321 ggatgatgcg cctgttaatt gagcgcgatg atgctgcaag tgccgcaggt cggccctcat
     1381 tgcttgatga cgaatttatt caaacgcata ccgtcggctt tgacgagcta cgccgtgacg
     1441 ttctcaattc cgagtggaaa gatatcgaac gtatttctgg actaagtcag acacaaatcg
     1501 ccgaactggc tgacgcatat gccgctgccg atcgcaccat tatctgttac ggaatgggga
     1561 tcactcagca cgaacatggt acccagaatg tacagcaact ggtcaatctg ctgttgatga
     1621 aaggtaacat tggcaagcct ggtgcgggta tctgcccact acgaggtcac tctaatgtac
     1681 agggcgaccg aaccgtcggt atcaccgaga aaccgtctgc agagtttctg gctcgcctgg
     1741 gtgagcgcta tggcttcacc ccacctcatg cacctggaca tgctgcaatt gccagcatgc
     1801 aagcaatatg cacggggcag gctcgagcat tgatctgcat ggggggcaac tttgcactgg
     1861 caatgccaga tcgggaagcg agcgctgtac cgttaacgca attagatttg gcggtacacg
     1921 tagccactaa gcttaaccgc tctcatctgc tgaccgcacg gcatagctat attctgccgg
     1981 ttctggggcg tagcgagatt gacatgcaaa aaagcggtgc gcaggcggta accgttgagg
     2041 attcaatgtc gatgattcat gcctcgcgtg gcgtgttaaa acccgccggt gtaatgctga
     2101 aatcagagtg tgcagtggtc gcgggaatcg cgcaggcagc actaccccac agcgtggtag
     2161 cctgggagta tctggtggaa gattatgatc gcattcgcaa tgacattgaa gctgtgttac
     2221 cagagttcgc cgactataac cagcgcatcc gacatcctgg aggttttcac ctgataaatg
     2281 cagctgctga aaggcgctgg atgacgccgt caggtaaggc taatttcatt accagcaaag
     2341 ggctgttaga agatccctct tcagcgttta acagtaagct ggtcatggcg acagtacgta
     2401 gccacgatca gtacaacacg acgatttatg gtatggatga tcgctatcga ggggtattcg
     2461 gtcaacgaga tgtggtcttt atgagtgcta aacaagctaa aatttgccgt gtaaaaaacg
     2521 gcgaaagagt taatcttatt gcgcttacgc cagacggtaa gcgcagctca cgccgcatgg
     2581 atagattaaa agtggtcatt taccctatgg ctgaccgctc actggtgacc tattttccag
     2641 aatcgaatca catgctaact cttgataacc acgatccatt aagtggcatt cctggttata
     2701 aaagtattcc ggttgaatta gaaccatcaa attaattcgc ctcctcattt cttctgctgt
     2761 catccgcaca gcagaagaat ttctcattga ctattatcgc gcaatttgct cacattgatt
     2821 aaattaaacc acacactata agatataaac ctctgcctac agctgtaaga aactccgctc
     2881 agtactgaag tgccagtcct atttcctctt ttctccagcc tgttatatta agcatactga
     2941 ttaacgattt ttaacgttat ccgctaaata aacatatttg aaatgtatgc gaccacagtg
     3001 aaaaacaaaa tcacgcaaag agacaactat aaagaaatca tgtctgtaat tgtggttgtc
     3061 ttattactga cccttacgtt gatagccatt ttttcggcaa ttgatcagct gggtatttca
     3121 gaaatgggtc gcattgcaag agatcttaca catt
//
LOCUS       DAZPDU010000303         3102 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZPDU010000303 DAZPDU010000000
VERSION     DAZPDU010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.307"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(59..442)
                     /gene="quuD"
                     /locus_tag="ACHURS_004439"
     CDS             complement(59..442)
                     /gene="quuD"
                     /locus_tag="ACHURS_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HFR1240002.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLITSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVVNNSN"
     gene            complement(528..668)
                     /locus_tag="ACHURS_004440"
     CDS             complement(528..668)
                     /locus_tag="ACHURS_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFR1240003.1"
                     /translation="MMFEFYMAELLRHRWGHLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            complement(665..1027)
                     /gene="rusA"
                     /locus_tag="ACHURS_004441"
     CDS             complement(665..1027)
                     /gene="rusA"
                     /locus_tag="ACHURS_004441"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFR1240004.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVTRIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            complement(1024..1314)
                     /locus_tag="ACHURS_004442"
     CDS             complement(1024..1314)
                     /locus_tag="ACHURS_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFR1240005.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTYFVDAEYAKECALEGMARTQVIWLKEGVIKA"
     gene            complement(1307..1477)
                     /locus_tag="ACHURS_004443"
     CDS             complement(1307..1477)
                     /locus_tag="ACHURS_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFR1240006.1"
                     /translation="MATPLIRVMNGHIYRVSNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(1477..1932)
                     /locus_tag="ACHURS_004444"
     CDS             complement(1477..1932)
                     /locus_tag="ACHURS_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcN family protein"
                     /protein_id="HFR1240007.1"
                     /translation="MTLPVDGIKLHRGNFAAIGQQIQPLLDAGQCLRLQVKPWREKRS
                     LSQNALSHMWYTEISEYLIARGKTFATPEWVKDAMKHTYLGYESKDRVDVVSGEVTTV
                     QSLRHTSELETGEMYIFLCKVEAWAMNIGCHLTIPQSCEYQQLRDKQEA"
     gene            2197..2574
                     /locus_tag="ACHURS_004445"
     CDS             2197..2574
                     /locus_tag="ACHURS_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240008.1"
                     /translation="MTNKENKDKYINKFKKELSKYIQPTAGVDIQLFNSKDGGGVIKA
                     TLNRSGKRKSSIAGNFTKLGEAITSSGQRVFGGDLSNVNFYGTNTIFDGDTIFLIKSP
                     DSEEWSSQKASKDVEGIVFGGKK"
     gene            2571..2999
                     /locus_tag="ACHURS_004446"
     CDS             2571..2999
                     /locus_tag="ACHURS_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240009.1"
                     /translation="MINITTKDGTQVSGEVRDLVILLETDGKTVSDYLQPIPPKKDTH
                     WSMIFIPVAIYILLNLVYIIAHPLFPEKYLKTSTSIFLFISFILSAIVAYFVFHKHKS
                     ATLSICSLVFLVLIVGANVGFISYSDLTQKATQKLDSLSK"
BASE COUNT          853 a          722 c          662 g          865 t
ORIGIN      
        1 atttttgggg gcgttgcaac gccccatttt taataatttt tagtaaacaa ttggcatatt
       61 aattagagtt attaacaacg atatccatct ctaaccggat atctaatgcc attaacatcc
      121 cttcaattat gccctcagcc ttctgtaacc ttttcccgat ataaccatca gagcagcaat
      181 gcttacctgc cagtgacatg aatgtcatac cgactacata ataatctact aataaatcgt
      241 gcaaatcgct gttgttcttt ttcagacggg ccatgcaccc gcaaataatc atcgcgtcat
      301 cgtcacaaca ttgcgggcga gattttactt ttgaagtaat taatccctta aaaccggcgg
      361 caatggacga ccaggtcaca tcttcatgat tattagccgc ccacgctccc caacgctcaa
      421 gaaccatctg aatatcacgc atcaacttac tccacaaaaa tcagaccaga acgccaatta
      481 caagcaaaaa tcaacaaaac agtattagtt gattgttatc tctgacttca tactcctgct
      541 cctgtcaggg ttttggcgta attcttcagt attcggtaat cggtcaaaac agaaccgggg
      601 aaacgatata agcgcagatg cccccagcgg tggcgaagaa gttctgccat ataaaactca
      661 aacatcattc attccccatt tcggtgatgg tcagttccag cctcccacct ttggtaacag
      721 gcatcttcac aacgcggtaa tcaacgacct gagcatcatc cagccagaaa cctgctttgg
      781 tgagtgcgtc aaaagcggct ttttgcagat tatccaggtc acggcgacgg cgatccggca
      841 tgtggcactc aatacggatt ttcactggca tagccaggcc gatatccagc attgcgtttt
      901 taatgattcg ggtgacgtta tcgcggtatg cctgcccttc tgcgctgatg tgtgtgcgcc
      961 cgcgattatg gcggtaatag cggttattgc tcggaggcca gggtaatgtg atgctgtagg
     1021 tattcacgcc ttaataaccc cctctttcag ccagataacc tgcgttctcg ccataccttc
     1081 cagcgcgcat tcttttgcat actcagcatc gacaaaatat gtgcggcggt cgatttcgtc
     1141 gtgacaggca gaacatgcaa tggtggcaat caggtctggc ggtttgatac cggtaccgca
     1201 caatccagcc agccggatat gtgccagtac tgacgtttca ggattgccat tacatacgcc
     1261 agggattctt acctggcatt cccgaccacg cgctgctttt ctcaaatcag ccatgattcc
     1321 tccttgctgc cagtcgcaac cattttttat caaccaggct agcggtatat ccgagcagtg
     1381 ttggtatttc ggatggcttc agctcaggct tacgcttacg acgatttgat actctgtaga
     1441 tgtgcccgtt catgacacga ataagcggtg tagccattac gcctcctgct tatcgcgcag
     1501 ctgctggtat tcacagctct gcggaattgt caggtggcag ccgatattca tcgcccaggc
     1561 ttcgactttg cacaggaaga tgtacatctc gccggtttcc agctctgacg tatggcggag
     1621 ggattggacg gtggtgacct cgccggacac gacatctaca cggtctttgc tttcgtagcc
     1681 gagataggtg tgcttcatcg cgtctttgac ccactcaggc gtagcgaagg tcttgccgcg
     1741 ggcgatgaga tattcgctga tctccgtgta ccacatgtgg ctgagcgcgt tctgcgacag
     1801 gctgcgcttc tcgcgccacg gcttaacctg caggcgtaag cattgcccgg catccagcaa
     1861 tggctgaatc tgctggccga tggccgcgaa gttaccgcga tggagtttga tgccgtctac
     1921 tggcagagtc atacggcctc cctaacggaa accgcagaat gcagaaaatc gcaggtgcat
     1981 ttctgcatct gtgacaaggt gatgagttca gattgtggtc gcatttaagt ccccttaaat
     2041 gcgcagaagt caccggagtt gttcaggctc cgatgagagg attatggcgg attgattacg
     2101 ataaatcaaa actatcttga agccaaaaca gtgatcttcg ctttaaaaac cttgtatact
     2161 ttcacctaat cacagccaga actatggatc taaccaatga ccaacaaaga aaacaaagat
     2221 aaatatatta acaagttcaa gaaagagctt tcaaaatata tacaaccaac tgctggagtg
     2281 gacattcaat tatttaactc taaggatggt ggtggtgtaa ttaaagccac tttgaataga
     2341 agtggaaaaa gaaaatcatc tattgctggt aattttacta aattaggtga ggcaattacc
     2401 tcgagtgggc agagggtttt tggtggtgat ctatcaaatg tgaattttta cggaacaaat
     2461 acgatttttg atggagatac aatattttta attaaatcac ctgattcaga ggagtggtca
     2521 tcacaaaaag cttccaaaga cgtagagggc atcgtatttg gagggaaaaa atgattaaca
     2581 ttactactaa ggatggcact caagtcagtg gtgaagtaag agatctagta attcttcttg
     2641 aaactgatgg aaaaacggtg tctgattatt tgcagccaat ccctcccaaa aaagatacac
     2701 actggagcat gattttcatt ccggtagcaa tttatatact acttaatctt gtatacatta
     2761 ttgctcaccc attatttcct gaaaaatatc taaaaacatc aacatctata tttctattca
     2821 tttccttcat attaagtgca atagtagcat attttgtttt ccataagcat aaatctgcca
     2881 ctctatccat atgcagctta gtgttcttag tgttaattgt aggtgctaac gtagggttta
     2941 tcagttacag cgacctaaca caaaaagcga cgcaaaaatt agatagtttg tctaaataat
     3001 tatgttgcca tgcgaagtat gcatggctgc atgtctgcct tccatttaaa atgggctaag
     3061 acctataacc ctaaatatta ttctttatta tcttctttac ca
//
LOCUS       DAZPDU010000304         3075 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZPDU010000304 DAZPDU010000000
VERSION     DAZPDU010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.308"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..1230
                     /locus_tag="ACHURS_004447"
     CDS             <1..1230
                     /locus_tag="ACHURS_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI
                     domain-containing protein"
                     /protein_id="HFR1240010.1"
                     /translation="DLWPVKEPDMLTVIMQRLTALEQSGEMGRKMDAFKERVIRNSLR
                     PPAVPGIGRTEKYSSRLFDPSVRLAADIRDNEGRVAGFESAYVALSRMKQHVQVYTDN
                     RQGWTDAINNAVQKGTAHDVFEPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLA
                     GGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGEGRVKG
                     SGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVSIAGEGRPWNPGAITDGR
                     VWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLPD
                     GKTELAVREIAGQERDRAAITEREAALPESVLRESQREREAVREVARENLLQERLQQM
                     ERDMVRDLQKEKTPGGD"
     gene            1250..1997
                     /gene="traX"
                     /locus_tag="ACHURS_004448"
                     /pseudo
     CDS             1250..1997
                     /gene="traX"
                     /locus_tag="ACHURS_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
     gene            2052..2612
                     /locus_tag="ACHURS_004449"
     CDS             2052..2612
                     /locus_tag="ACHURS_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFR1240011.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            2742..2954
                     /locus_tag="ACHURS_004450"
     CDS             2742..2954
                     /locus_tag="ACHURS_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFR1240012.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
BASE COUNT          713 a          763 c         1016 g          583 t
ORIGIN      
        1 gatctgtggc cggtaaagga gccggacatg ctgacggtga tcatgcagcg cctgacggca
       61 ctggagcagt ccggtgagat gggccggaaa atggatgcct ttaaggagcg ggtgatccgc
      121 aacagtctgc ggccccctgc cgtgcccggt atcggacgca cggagaaata cagcagccgg
      181 ttgtttgacc cgtccgtcag actggctgcg gatatccggg ataacgaagg gcgggtggcc
      241 ggctttgagt cagcctacgt ggccctgtcg cgtatgaagc agcatgtgca ggtgtacacc
      301 gataaccgtc agggctggac ggatgccatc aacaatgccg tacagaaagg aactgcccac
      361 gatgtgtttg agccgaaacc ggaccgggag gtcatgaatg cagagcggct gttcagtacg
      421 gcgcgggagc tgcgggacgt ggcggcaggc cgtgccgttc tccgtcaggc ggggctggcc
      481 gggggagaca gtcctgcacg gtttattgct ccgggacgta aatatccgca accgtatgtg
      541 gcactgccgg cgtttgaccg taacggcaag tccgccggta tctggctgaa cccgctgacc
      601 acggatgacg gaaacgggct gcggggattc agcggtgaag gtcgggtaaa aggcagcggg
      661 gatgcgcagt tcgtggccct gcagggcagc cgtaacggag aaagcctgct ggctgataat
      721 atgcaggatg gcgtccggat tgcccgtgat aatcctgaca gtggtgtggt ggtaagcatt
      781 gccggtgaag gccgtccgtg gaatcccggt gccatcaccg atggccgcgt gtggggggat
      841 atcccggaca acagcgtcca gccgggagcc ggaaatggcg aaccggtcac ggcagaggta
      901 ctggcacagc ggcaggctga agaggccatc cgccgtgaaa cggaacgtcg cgcagatgaa
      961 attgtccgta aaatggcaga gaacaaacct gacctgccgg acggcaaaac agagctggct
     1021 gtcagggaaa ttgccgggca ggagcgtgac cgggctgcca taactgaacg ggaagccgcg
     1081 ctgccggaga gtgtgctgcg tgaatcacaa cgggagcggg aagcggtccg ggaggttgcc
     1141 cgggaaaatc tgctgcagga gcgtctgcag cagatggagc gggatatggt tcgtgacctg
     1201 cagaaagaga aaaccccggg cggagactga tacaggaaga caaacactga tgacaacgga
     1261 taacacgaac acgacacgta acgattcact ggctgcacgg accgatacct ggttgcagtc
     1321 atttctggtc tggtcacccg gacagcggga tatcatcaaa acggtggcac tggtgctgat
     1381 ggtactggac cacatcaatc tgatattcca gctgaagcag gaatggatgt ttctggccgg
     1441 gcgaggagcc tttccgcttt ttgccctggt gtgggggctg aatctgtccc gtcatgcgca
     1501 tatccggcaa ccggccatta accggctgtg gggatgggga attattgccc agttcgcata
     1561 ttatctggca ggctttccct ggtatgaggg gaatatcctg tttgcctttg cagtggcggc
     1621 acaggtgctg acgtggtgtg aaacgcgcag tgggtggcgt acagcagcgg ccattcttct
     1681 gatggccctg ttgggggcct ttgtccggca ccagttacgg cattgccggg ctgctgatgc
     1741 tggcagtcag ccaccgactg taccgggcgg aagacagagc ggaacgtctg gcgctggtcg
     1801 cctgcctgct ggctgttatt ccggcgctta accttgccac cagtgatgca gcggcggtgg
     1861 ccggtcttgt gatgacggtg ctgaccgttg gtctggtgtc gtgtgcagga aaatcattac
     1921 cccgtttctg gcccggggat tttttcccgg tattctacgc ctgtcatctg gctgtgctgg
     1981 gcgttctggc gctgtgacgg gtgtggtatc tttgaccgta acaggatgat tcgtcagagg
     2041 cagaacacag catgacagag cagaagcgac cggtactgac actgaagcgg aaaacggaag
     2101 gggagacgcc tgtccgcagc cggaaaacca tcatcaatgt caccacgcca ccaaagtgga
     2161 aggtgaaaaa gcagaagctg gccgagaaag cagcccggga agcagagctg gcggcaaaaa
     2221 aagcgcaggc cagacaggca ctgtccattt atctgaacct gcccacgctg gatgaggccg
     2281 tgaataccct gaaaccctgg tggccgggat tatttgacgg tgacaccccc cggcttctgg
     2341 cctgcggtat ccgggacgtg ttactggaag acgtggcgca gcggaatatc ccgctctcgc
     2401 ataaaaaact gcgcagggcg ctgaaggcca tcacccgttc agaaagctat ctgtgtgcca
     2461 tgaaagccgg tgcctgccgg tatgacacgg aagggtatgt gacggagcat atttctcagg
     2521 aggaggaagc gtatgcggca gcgcgtctgg ataaaatccg ccgccagaac cggataaagg
     2581 cagaacttca ggccgtgctt gatgagaaat aaaaaaaagc ctccatcaga gaggaggcag
     2641 ggaaataagg cttaaaagga aataacttcc gcttccgaaa ggaagccagt ccactgaatt
     2701 atttatcaca ataatactga caactaaagg gggacaagaa aatgaatgga ttcagaaaca
     2761 gttcacggaa cggccaggtc tggcgttacc agcgtgccgg cgggcgggcc gttattctgg
     2821 aagtcagtgg acgctggatg gaagcggcag aagcatggcg acgggctgcc tgtgttgcgc
     2881 ccaggacaga ctggcagcag tttgcccgaa aaagggctga acactgccac cggcgctgcc
     2941 ggggaagggt gtaacgaaaa aagcagcctt cattactcca ggagtcagaa ggctgaaagg
     3001 agtttatgag atttgctttt tttaatatcc ggtcgggcag tatgaccgga cagttctgag
     3061 tagcacagag ttttt
//
LOCUS       DAZPDU010000305         3073 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZPDU010000305 DAZPDU010000000
VERSION     DAZPDU010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.309"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(72..147)
                     /locus_tag="ACHURS_004451"
     tRNA            complement(72..147)
                     /locus_tag="ACHURS_004451"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(112..114),aa:Asn,seq:gtt)
     gene            complement(248..1735)
                     /gene="yeeO"
                     /locus_tag="ACHURS_004452"
     CDS             complement(248..1735)
                     /gene="yeeO"
                     /locus_tag="ACHURS_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFR1240013.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
     gene            1896..1971
                     /locus_tag="ACHURS_004453"
     tRNA            1896..1971
                     /locus_tag="ACHURS_004453"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1929..1931,aa:Asn,seq:gtt)
     gene            complement(2009..2959)
                     /gene="cbl"
                     /locus_tag="ACHURS_004454"
     CDS             complement(2009..2959)
                     /gene="cbl"
                     /locus_tag="ACHURS_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFR1240014.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
BASE COUNT          903 a          812 c          645 g          713 t
ORIGIN      
        1 gtaaccgggt aaaaataatt tttatttttt aactgttttg agactcatag agatgtctca
       61 aaactaaaat ttggctcctc tgactggact cgaaccagtg acatacggat taacagtccg
      121 ccgttctacc gactgaacta cagaggaatc gtgagaacga ggcgaatatt agcgatgccc
      181 acccacaatg tcaaagcctg ttttttaaat ttgaaatcgt ttgctgaaat aatctgcatt
      241 ttgtcgttta ttccgacaca actggctttt tttcacactt ttgcggttcg gatcgagggt
      301 atttccacag ccaacgtcca gtaaccattc gccagtaaaa cagcacggcc cgcacagccc
      361 agtcggcaaa cattcccatc cagacaccaa ccacacccca gccaagcatg atgcccagca
      421 cataaccgac gacaacccgg caaccccaca tgctcaacat agaaacccac atggcgtaac
      481 gggcatcgcg agcaccttta aatccagcgg gtagcaccca tgaggcggac caaataggca
      541 taaataaagc atttagccaa atcagaatca caacgacatg tttaacctgt ggatcctggg
      601 tgtaaaacga tgccataacc ccggcaaagg gagccgttag ccaggcgatg gccgttaatc
      661 caagagtgga aagccagaac acatgccgca actgaatctc tgcttgcgct atctgcccta
      721 cccccaacct tcggcctgta atgatcgtag aagcagagcc gagcgcactt ccgggtaagt
      781 tgataagagc cgcaattgaa aacgcgataa aatttccggc aataacactg gtccccatcc
      841 cggcaacgaa catttgggtt aataaccgac cactggtaaa taacactgat tcgacactcg
      901 cgggaatacc aatccccatg acttcccaga taatgctaaa attcagcggt ttaaaatagc
      961 tctttaacga aatccttagc gcaggattaa aaccaatcgc cagcacccac aaaattgcaa
     1021 ctgcgccaat ataacgagaa atggttaaac ccagccctgc cccgacaaat cccagtcccg
     1081 gccaggagaa aaggccgtaa atcaatatgc cgctaataat aatattaaga atattcaggc
     1141 taccgttaat caatagcggt attttcgtat tccctgcacc acgaagtgcc ccgctaccaa
     1201 taagagtgat ggcagctgct ggataactga gtaccgtcag ctccagataa gtcaacgcca
     1261 gtgctttaac ttctgtcgtg gcatcacccg cgacgaaatc aataatttgt tcgccaaaat
     1321 gatgaataag cgttgccaac agtacggcaa acaacgtcat gatcaccaat gactgccgcg
     1381 tcgccaccct cgctcgtcgt cgatcccgct taccgagact aaatgccaca acgacagtag
     1441 taccaagatc gatagcagca aaaaaagcca taatgaccat attgaagctg tccgccaatc
     1501 ccacgccggc catcgcatct tttcccagcc agctgaccag aaaagtgctc agaaccccca
     1561 tcaacaggac acaggcattc tccatgaaga taggaacagc aagcggggtt atctcgcgcc
     1621 agaacaacac tttgtagctc ttgcgtttag cgtgccagcg agtgccgtga acaacctggc
     1681 gtaaagcaga ggagatattc aaagccgacc ttaattgcag aaagtgaaac cacatttcaa
     1741 ataatgaggg agaatcagca aagctgcaaa gattttcgcc aacaaattgt ctgcaaatgc
     1801 aacaaactgt tgatagaaac ggcaaacagt tggggaattt aaaaatcggg tttgacaaaa
     1861 gatttttcgc cgttaagatg tgcctcaaca gcgattcctc tgtagttcag tcggtagaac
     1921 ggcggactgt taatccgtat gtcactggtt cgagtccagt cagaggagcc aaatttctga
     1981 aaagcccgct tttatagcgg gctttttgct atatctgata atcaatttcc tcttcactgt
     2041 tttccatcac ctgccgcttg atatcctcaa ctgacagtcc tgcattacaa agttccagaa
     2101 agcgccagac atagttacgc tgaagttgtc ctcgcttcag tcccaaccag acagtattag
     2161 catcgaaaag atgccgcgta tccagacgga ttaaattctc ttcctcttgt tcgccgctgg
     2221 attgttcggc aactaatccg atcccaagcc caagagcaac ataggtttta atgacatcag
     2281 aatcctgcgc acttaataca atatctgcca gcaaaccttt gcgggcaaat gcgtcatcaa
     2341 tacgtgagcg ccccgtaatc ccctgtcggt aagtgattaa cggccacttc gctattgatt
     2401 ccagcgtcaa tggtgtaatt tgcgttaagg gatgatcgag tggaacaagc aaactatggt
     2461 gccaacgaaa ccacgggaag gcgacgagct gcgggtcatt actcaaacgc tcgctggcga
     2521 taccaatatc agcttcgcca ttttgcaaca acgtcgcaat ttcctgtggc gtcccctgga
     2581 ttagctcgag ccgaacctcc gggaaaagtt cgcgaaaagc tttaatgacc tctggcaagc
     2641 tataacgtgc ctgagtatgc gtcgttgcaa tagtgagaac gccagacgta tcgttggtaa
     2701 acaggtctgc aagccgacga acattactgg cttcattcag aatacgttct gcaatgacca
     2761 gtaatgcttt gcccggttca gtcatgccca gcagtcgctt acctcgtcga acaaatattt
     2821 cgatgccaag ttcatcctcc agttcccgaa tatgacggct gacgccggac tgtgaggtaa
     2881 aaagcatatt cgcaacctct gtcaggttgt aatcctgacg tgcagcctcg cggattatct
     2941 ttagttgttg gaaattcacg gtaaactccg ggcagttcag atttcccgtt attgttaaag
     3001 tctaatgccc ggcataacaa ataataaaaa cccgcatctt attccatccc gatataacgc
     3061 ttagctcacc aat
//
LOCUS       DAZPDU010000306         3068 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZPDU010000306 DAZPDU010000000
VERSION     DAZPDU010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.310"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            105..437
                     /gene="hdeA"
                     /locus_tag="ACHURS_004455"
     CDS             105..437
                     /gene="hdeA"
                     /locus_tag="ACHURS_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFR1240015.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            553..879
                     /gene="hdeB"
                     /locus_tag="ACHURS_004456"
     CDS             553..879
                     /gene="hdeB"
                     /locus_tag="ACHURS_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFR1240016.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            943..1590
                     /locus_tag="ACHURS_004457"
     CDS             943..1590
                     /locus_tag="ACHURS_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFR1240017.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNMVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(1632..2162)
                     /locus_tag="ACHURS_004458"
     CDS             complement(1632..2162)
                     /locus_tag="ACHURS_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFR1240018.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(2318..2884)
                     /gene="slp"
                     /locus_tag="ACHURS_004459"
     CDS             complement(2318..2884)
                     /gene="slp"
                     /locus_tag="ACHURS_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFR1240019.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT          894 a          607 c          671 g          896 t
ORIGIN      
        1 ttgatatttt atatcaacat gacatataca gaaaaccagg ttataacctc agtgtcgaaa
       61 ttgattcgtg acggctcttt cactttatag ttgaggatat tacgatgaaa aaagtattag
      121 gcgttattct tggtggtctg cttcttctgc cagttgtgag caatgcagcg gatgcgcaaa
      181 aagcagctga taacaaaaaa ccggtcaact cctggacctg tgaagatttc ctggctgtgg
      241 acgaatcctt ccagccaact gcagttggtt ttgctgaagc gctgaacaac aaagataaac
      301 cagaagatgc ggttttagat gttcagggta ttgcaaccgt aaccccagct atcgttcagg
      361 cttgtactca ggataaacaa gccaacttta aagataaagt taaaggcgaa tgggacaaaa
      421 ttaagaaaga tatgtaattc cgggaatgcg ttacatcgta cttccttgca tattgaacag
      481 gccggaatat cttctttaaa agcagctatt cctcctgttc atatataatc tctatattga
      541 atgggttaca aaatgaatat ttcatctctc cgtaaagcgt ttatttttat gggcgctgta
      601 gcggctttgt cactggtgaa cgcacaatct gcgctggcag ccaatgaatc cgctaaagat
      661 atgacctgcc aggaatttat tgatctgaat ccaaaagcaa tgaccccggt tgcatggtgg
      721 atgctgcatg aagaaacagt atataaaggt ggcgataccg ttactttaaa tgaaaccgat
      781 ctcactcaaa ttcctaaagt gatcgaatac tgtaagaaaa acccgcagaa aaatttgtat
      841 accttcaaaa atcaagcatc taatgacttg ccgaattaat gaggtacaag taaaaaggag
      901 tagcaagttg agccatcttg ctgctccttt ttgcattttt atatgacagc agaatttatt
      961 atacgtctta tactcgcggc aattgcctgt ggcgctattg gcatggaaag gcaaatgcgc
     1021 ggcaaaggag cagggttacg cacacatgta ttaattggca tgggaagcgc cctgtttatg
     1081 attgtttcga aatatggttt tgctgacgtg ctgtctttag atcacgtcgg actcgacccc
     1141 agccgtattg ctgctcaggt ggtgacgggc gtcgggttta tcggtgcagg taacattctc
     1201 gttcgtaacc aaaatattgt aggtctgacg acggcagcgg atatctgggt gaccgccgcc
     1261 ataggtatgg ttattggcag cggtatgtac gaactgggta tttatggttc agtgatgacc
     1321 ttgttggtgc tggaagtctt ccatcaatta accttccgcc tgatgaacaa aaattaccat
     1381 ttacagctga cattagtgaa cggtaatatg gtatcaatgc tcgactggtt taagcagcaa
     1441 aaaataaaaa ctgatttagt ttcgttgcag gaaaatgaag atcatgaagt ggtcgccatt
     1501 gatatccagc ttcatgccac aacgtcgata gaagacctgt tgcgattatt aaaaggtatg
     1561 gcaggggtga aaggcgtttc aattagctaa tctgtgtggc agcgtagcca gactcaccgt
     1621 aagcctgaaa ttcacaccag ataatcaata tgctgatggc gtaccagctc attgatccgc
     1681 ttaagattga ggatcatcat gatattgtgt ttatggcagt agaacgtttt tagcgatatt
     1741 ttcagtttct ccagaatatc ttcagttgtc atttgattcg caatgcagct cagaacctga
     1801 tgttgcttgc gcgaaagtga cacattcgct tcaggcacag agcagtaact gccattgatg
     1861 agtgcgacaa aatcaatgat agtgatagtc cgcggaacaa aggtcaccgg tactttcccc
     1921 agacagcgtt taatattaaa gggggagaaa acaatgacat ggacaggttt taatcgttca
     1981 atttgagtga gaaaattaga atgaaataca ttattcatta atgtatcaat aatgacgaag
     2041 gcattctgat gcaacattac gtcgatctct tcttcgttct gtatatgaac gacattacct
     2101 ttactcagaa tgtttttcat cgcggtgaag aacatcgtat ccctggtaat tataagaaac
     2161 ataaccgact cctggtgcgg acattaactt tataatcaat ttgccaaagg taattaacag
     2221 gacagggctt ataataataa tagttagctt actgtatatt aattttatgt aatacatgat
     2281 gcagatgctg agaaagcatc tgcatctttc ggtggtgtta tttgaccagc tcaggtgtta
     2341 cctgactcac cgcattggtg tagtaaggcg caccccagcc cggttccggc cagaatgcac
     2401 catagccgta atcccacagg ttataggtgg tattaaccac ttctctcaaa tgccacacct
     2461 ggatgccctg catattcact tccaggaagt tatacgggac tttattgata aagccaggtt
     2521 gttcaccctg aatggtaccg aggatggtaa caaagtgatt acgatagttc actgggtcaa
     2581 ggaagccgct ttgtctggcg agcagtcggc cctgatagtt ggcttcaata tcaggtttcg
     2641 catagctatc caacggtaat acagcgattt ctaacaacgt atccgttttg ccattgataa
     2701 cgttgataac cttcccacca aagcgcgctt gttgaccaac atataacccc ggctggttat
     2761 gaacagcaac aaaacttttt tgaatatcag gttggttatt gcctttgata ttttgcggaa
     2821 ttgaactaca tgcggcaagc aaaaatgaaa ggctgaggat gagtgcacct tttgtcatgt
     2881 tcatgttact atccttatca acaaatatta aaaccataat agatatttct ttatagtttt
     2941 catctgattc tgaggcgata tgcctgatag taacgtaaac ctgtctgatg tttatatccc
     3001 ttttgtttag ttcaattcca ttgattttgg ggcgtggcat gagtatccat cgccccataa
     3061 tataatcc
//
LOCUS       DAZPDU010000307         3047 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZPDU010000307 DAZPDU010000000
VERSION     DAZPDU010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.311"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..174
                     /locus_tag="ACHURS_004460"
     CDS             <1..174
                     /locus_tag="ACHURS_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240020.1"
                     /translation="QAKALMAQYRVPVVVEVILERVTNISMGSELDNVMEFEDIADNA
                     VDAPTETCFMHYE"
     gene            187..1038
                     /gene="hyi"
                     /locus_tag="ACHURS_004461"
     CDS             187..1038
                     /gene="hyi"
                     /locus_tag="ACHURS_004461"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFR1240021.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYFLNRVVATGKVMKPVLAQR
                     QKVVSPP"
     gene            957..2135
                     /gene="allB"
                     /locus_tag="ACHURS_004462"
                     /pseudo
     CDS             957..2135
                     /gene="allB"
                     /locus_tag="ACHURS_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
     gene            2192..>3047
                     /locus_tag="ACHURS_004463"
     CDS             2192..>3047
                     /locus_tag="ACHURS_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HFR1240022.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPYFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATG"
BASE COUNT          696 a          711 c          836 g          804 t
ORIGIN      
        1 caggcgaaag ccttaatggc gcaatatcgg gtaccggtag tcgtggaagt tattcttgag
       61 cgtgtgacca atatttcgat gggcagcgaa ctggataacg tcatggaatt tgaagatatc
      121 gccgataacg cagtggacgc accgactgaa acctgcttca tgcactatga ataagggaga
      181 taaataatgt tacgtttctc tgctaattta tcgatgttat ttggtgaata tgattttctc
      241 gcccgttttg agaaagctgc gcagtgtggt tttcgcggcg ttgaatttat gtttccttat
      301 gactacgaca ttgaagaatt aaaacaggtg ctggcgagta ataaactcga acatacgctg
      361 cacaatctac cggcgggtga ctgggcggcg ggggagcgcg gtattgcctg tattcctggc
      421 cgtgaagaag agtttcggga tggcgtagca gcagcgattc gttatgcccg tgcgctgggg
      481 aataaaaaaa ttaactgtct ggtcggtaaa acgccggctg gtttcagcag tgaacagatt
      541 cacgcaacgc ttgtagaaaa cctgcgttat gccgcgaata tgctgatgaa agaagatatt
      601 ttattactga ttgaacctat taaccatttt gatattcctg gtttccatct caccggaact
      661 cggcaggcgc tgaaattgat tgatgatgtt ggttgctgca atttgaaaat tcagtatgac
      721 atttatcaca tgcagcggat ggaaggtgaa ttaaccaaca ccatgactca gtgggccgat
      781 aaaattggtc acctgcaaat tgctgataat ccgcatcgcg gcgaaccggg gaccggagaa
      841 attaattatg attatctctt taaggtaatc gaaaattctg actacaacgg ttgggttggg
      901 tgtgaatata aaccccaaac caccacggaa gccggtttac gctggatgga tccgtacttt
      961 ctgaaccggg tcgtagccac tgggaaggtt atgaaaccgg tactcgcgca gcggcaaaag
     1021 gtggtatcac caccatgatc gaaatgccgc tcaaccagct gcctgcaacg gttgaccgcg
     1081 cttcaattga actgaagttc gatgccgcta aaggcaagct gactatcgat gcggcacaac
     1141 tcggtggcct ggtgtcttac aacatcgacc gtctgcatga gctggatgaa gtgggcgttg
     1201 tcggcttcaa atgcttcgtt gcgacctgtg gcgatcgcgg tatcgacaac gacttccgtg
     1261 atgtaaacga ctggcagttc ttcaaaggtg cgcagaagct gggcgaactg gggcagccgg
     1321 tgttggtgca ctgcgaaaac gcgctgatct gtgacgcact gggcgaagaa gcgaaacgtg
     1381 aaggtcgcgt aactgcccat gactatgtgg cttcgcgtcc ggtatttacc gaagtggaag
     1441 cgattcgccg cgtgctgtat ctggcgaaag ttgccggttg ccgtctgcac gtttgccata
     1501 tcagcagccc ggaaagtgtt gaagaagtga ctcgtgcacg tcaggaaggt caggatgtta
     1561 cctgtgaatc ctgcccgcat tactttgtac tggataccga tcagttcgaa gaaatcggta
     1621 ctctggcgaa gtgttcaccg ccgatccgcg atctggaaaa ccagaaaggc atgtgggaaa
     1681 aactgtttaa cggtgaaatc gactgcctgg tttccgacca ctcaccatgc cctccggaaa
     1741 tgaaagccgg caacatcatg gaagcatggg gcggtattgc cggtctgcaa aactgtatgg
     1801 acgtgatgtt cgatgaagcg gtacagaaac gcggaatgtc tctgccaatg ttcggcaaat
     1861 taatggcgac taacgcagca gatattttcg gtctgcagca aaaaggccgt atcgccccag
     1921 gaaaagatgc cgacttcgtc ttcattcagc cgaatagcag ctatgttctt accaatgacg
     1981 atctggaata tcgccacaaa gtcagcccgt atgttggccg taccattggc gcgcgtatca
     2041 cgaaaaccat cttacgtggt gatgtgattt acgacattga acagggcttc cctgttgcgc
     2101 cgaaaggtca atttatcctt aaacatcagc agtaatctgg cccctgcaat gcccgtcctt
     2161 gcggcgggca ttctccggtt aaggtgtgtt tatgttcaat tttgcagtca gccgcgaaag
     2221 cctgttatca ggatttcagt ggtttttctt tattttttgc aacacggttg tggttcctcc
     2281 tacgctactt tctgcttttc agttgccgca aagtagcctg cttacgctca cgcaatatgc
     2341 ttttcttgct accgcactgg cctgcttcgc tcaggcgttc tgcggtcatc gtcgcgctat
     2401 tatggaaggg ccgggtggcc tgtggtgggg aaccatcctt actatcaccc ttggcgaagc
     2461 atcgcgcggg acaccgatca acgatatcgc taccagtctg gcggtgggga ttgcactctc
     2521 cggcgtgctg acgatgttga ttggttttag cggattaggc catcgcctgg cacggttatt
     2581 tacgccgtcg gtgatggtct tgtttatgtt gatgctgggc gcacagctga ccactatctt
     2641 tttcaaaggt atgctcgggc tgccgtttgg catagccgac ccatatttta aaattcagtt
     2701 accaccgttc gcgctctcgg tggcggtgat gtgcctggta ctggcgatga ttatctttct
     2761 gccgcaacgt tttgcccgtt atggcctgct ggtcggcacc ataaccggct ggttgttgtg
     2821 gtacttttgc tttccttctt cgcattcgct ctccggtgag ttgcactggc agtggttccc
     2881 gctcggcagt ggcggtgctt tgtcgccggg aattattctg acggcggtga ttacaggtct
     2941 ggtaaatatc agcaatacct acggtgcgat tcggggcacg gatgtttttt atccgcagca
     3001 gggcgcgggg aatacgcgtt atcgtcgcag ctttgtggcg accggat
//
LOCUS       DAZPDU010000308         2992 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZPDU010000308 DAZPDU010000000
VERSION     DAZPDU010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.312"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..1076
                     /gene="bglB"
                     /locus_tag="ACHURS_004464"
     CDS             <1..1076
                     /gene="bglB"
                     /locus_tag="ACHURS_004464"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFR1240023.1"
                     /translation="RLFDEMAQAGIRPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEH
                     YARTVFTRYQHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARA
                     VKACHSLIPEAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYM
                     QRFFRDHNITIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPN
                     PHLKSSEWGWQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIA
                     YLNDHLVQVNEAIADGVDIMEYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLT
                     RTRKKSFGWYAEVIKTRGLSLKK"
     gene            1162..2778
                     /gene="bglH"
                     /locus_tag="ACHURS_004465"
     CDS             1162..2778
                     /gene="bglH"
                     /locus_tag="ACHURS_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HFR1240024.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            2805..>2992
                     /locus_tag="ACHURS_004466"
     CDS             2805..>2992
                     /locus_tag="ACHURS_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HFR1240025.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVS"
BASE COUNT          838 a          632 c          744 g          778 t
ORIGIN      
        1 atcggctgtt tgatgaaatg gcgcaggcgg ggatcaggcc gctggtaacg ttatcccatt
       61 acgaaatgcc atatgggctg gtgaaaaact acggcggttg ggctaatcgg gcggtcatcg
      121 atcacttcga gcattacgcc cgcacggtct ttacccgcta ccaacataaa gtagcgttat
      181 ggctgacgtt taatgaaatc aatatgtcgt tacacgcgcc attcacgggc gtggggctgg
      241 cagaagagag tggcgaggcg gaagtttatc aggcgatcca tcatcaactg gttgccagtg
      301 cgcgggcagt taaagcctgt catagcctga tccccgaagc gaaaatcggc aatatgcttc
      361 tcggtgggct ggtttacccc ctcacctgcc agccacagga tatgttgcag gccatggaag
      421 agaaccggcg ctggatgttc tttggtgatg ttcaggcgcg tggccagtat cccggctata
      481 tgcagcgttt cttccgcgac cacaatatca ccattgagat gactgaaagt gacgcagaag
      541 atttaaaaca tactgtagat ttcatctctt ttagttatta catgactggt tgtgtttccc
      601 acgacgaaag cattaataaa aatgcgcagg gcaacatact gaatatgatc cccaatccgc
      661 atctgaaaag ttcagagtgg gggtggcaaa ttgatccggt tggattacgg gttctgttaa
      721 atacgctttg ggatcgttat caaaaaccgt tatttattgt cgagaacgga ttaggcgcaa
      781 aagacagcgt tgaagcggat ggttcgatac aggacgatta tcgaattgcc tatttaaacg
      841 atcacctggt acaggtaaat gaagcgattg ccgatggtgt ggatattatg gagtacacca
      901 gttgggggcc aattgattta gtcagtgcat ctcattcaca aatgtctaag cgctacggct
      961 ttatttatgt ggatcgtgat gataatggcg aaggaagcct cacaagaacg cgtaagaaaa
     1021 gcttcggatg gtatgcagaa gtgatcaaaa cgcgggggct gtcattaaaa aaataaccaa
     1081 taaagcacct taattatcgt cgcattcaga acagtctgga tgcgatacgt taattctttc
     1141 tttgcaccat aaagggatat tatgtttaga cgaaatctta ttacctctgc catcttatta
     1201 atggcaccgt tagccttttc tgcacaatca ttggctgaat cattaacggt ggaacaacgc
     1261 cttgagttat tagaaaaagc gttaagagaa acgcaaagcg aactcaaaaa gtataaagat
     1321 gaagagaaga aaaaatatac gccagcgacg gtgaatcgta gcgtaagtac gaatgatcaa
     1381 gggtatgccg ccaatccgtt cccgaccagt agtgccgcaa aacctgatgc tgtactggtc
     1441 aaaaatgaag agaaaaatgc cagtgagaca ggttcgattt attcttccat gactctgaaa
     1501 gatttcagta agtttgtgaa agatgaaatt ggctttagtt acaacggcta ttaccgttct
     1561 ggttggggga ccgcctctca tggttcacct aaatcatggg cgattggttc tctgggccgc
     1621 tttggtaacg aatactccgg ctggtttgat ttgcagttaa aacaacgtgt ctacaacgaa
     1681 aacggcaaac gggttgatgc cgttgtgatg atggatggta acgttggtca gcagtactct
     1741 accggctggt ttggcgataa cgccggtggc gagaacttta tgcagttctc cgatatgtac
     1801 gttaccacca aaggtttcct gccctttgcg ccagaggctg atttctgggt gggtaaacac
     1861 ggtgcgccga aaattgaaat ccagatgctg gactggaaaa cgcagcgtac tgatgccgca
     1921 gcgggtgtag gtctggaaaa ctggaaagtc ggtccgggta aaattgatat cgcgctggtt
     1981 cgcgaagatg tcgatgatta cgatcgcagc ctgcaaaaca aacagcagat taataccaat
     2041 actattgatt tacgctataa agatatcccg ttatgggata aagcgacctt aatggtgagt
     2101 ggtcgttatg tcacggcaaa cgaaagcgca tcggaaaaag ataatcagga taataacggg
     2161 tattatgact ggaaagatac ctggatgttc ggcacatctt taacgcagaa atttgataaa
     2221 ggtggcttca acgaattctc cttcctggtt gcgaataact ctatcgccag taactttggc
     2281 cgttatgctg gcgcaagtcc atttaccacc tttaatggtc gttattatgg tgatcacacc
     2341 ggcggaacag cagttcgtct gacctcgcag ggcgaagcct atatcggcga tcattttatt
     2401 gtggctaacg cgattgttta ctccttcggt aacgatattt atagctacga aacaggcgca
     2461 cactctgatt tcgaatctat tcgtgcggtt gttcgcccgg cctatatttg ggaccaatat
     2521 aaccagacag gtgttgaact gggctatttc acccagcaaa acaaagatgc gaatagtaat
     2581 aaatttaatg agtctggtta taaaaccacg ctcttccata cctttaaagt caataccagt
     2641 atgttgacct cgcgtccgga aattcgtttc tacgccacgt atatcaaagc tctggaaaac
     2701 gaactggatg gcttcacctt tgaagacaat aaagacgacc agtttgctgt cggtgcgcag
     2761 gctgaaatct ggtggtaatg gaacttaatg aaaggaatat tacgatgaat ataaaaattg
     2821 ctgctttaac gctggctatt gccagcggta tttctgctca gtgggccata gcagcggata
     2881 tgccagccag cccggcaccc actattccgg ttaaacagta tgtgactcag gtcaatgccg
     2941 ataacagcgt gacctttcgc tactttgccc ctggggcaaa aaacgtctcc tt
//
LOCUS       DAZPDU010000309         2939 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZPDU010000309 DAZPDU010000000
VERSION     DAZPDU010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.313"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            37..264
                     /locus_tag="ACHURS_004467"
     CDS             37..264
                     /locus_tag="ACHURS_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFR1240026.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDTHHGPYE"
     gene            complement(265..639)
                     /locus_tag="ACHURS_004468"
     CDS             complement(265..639)
                     /locus_tag="ACHURS_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MysB family protein"
                     /protein_id="HFR1240027.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGHAAADEWDER"
     gene            complement(722..1768)
                     /gene="mdtG"
                     /locus_tag="ACHURS_004469"
                     /pseudo
     CDS             complement(722..1768)
                     /gene="mdtG"
                     /locus_tag="ACHURS_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
     gene            complement(2099..2674)
                     /locus_tag="ACHURS_004470"
     CDS             complement(2099..2674)
                     /locus_tag="ACHURS_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR1240028.1"
                     /translation="MSRTFAYCRVSVETSTTENQIQAIKKAGYDIPKSRVIEEVVSGS
                     VSAMDRDGFANLVNHKLEEGDTLVVLKLDRLGRDNIDLQNTVTLLNDKGVKLICLDLP
                     VKDLSSSEGKLVLQLFSTFAEFERNRIRERTKQGLERARVQGKVLGRPIASDTTRAVL
                     EYKERGLSQSQVAKVLNLSVRTVSRHWTKTL"
BASE COUNT          762 a          695 c          689 g          793 t
ORIGIN      
        1 cgttatgccg gtattttttt acggaagagg tatcaaatgc gtttaattgt ggtgagcatc
       61 atggtgacct tgctgagtgg ctgtggcagc atcattagcc gcactatacc ggggcagggg
      121 cacggcaacc aatattatcc cggtgtgcaa tgggatgtgc gtgactccgc ttggcgttat
      181 gtcacaatcc ttgatctgcc attctccctg gtttttgata ctttactgct gccgatcgac
      241 actcatcatg gcccgtatga gtgattaacg ttcatcccac tcatcagctg cagcgtgtcc
      301 ttcctcggta tccagcggtg gctcaagctg aaattccccc tcgtcccatt catgtaatgt
      361 attctcttcc tgccactcct ggcggatctc tatctcatca tagtcgccat caaaaacact
      421 ttgcgcggct tcaccgctaa gcataggtaa acattcacct tcttcccctt cgtcggcaaa
      481 aaactcaact tgccacatga tgtcgccgtc ctgcaaaacg tatttttggg cattgaactg
      541 ttgcacattc gcatcttcgg cgtcgatgcc ggggttgtct gcaagaaatt cttcgcgtgc
      601 agcgtcaatg gcttcttcaa gcgttgcgta catggtcatc acgattctcc ctttgagttg
      661 atgaggtttc agggaaaagg atagctgatt ctccgctttt gcaagtatga aaggcgaaaa
      721 atcagttcga tacctggggt attcgacgac gacgtagact gttccatgaa tagactgcgt
      781 tgaataacac tacgccagcg gtgacgagaa ataccgctct gaaaccgtag ttcgctgaaa
      841 tcgctgctcc catcaatggt ccggtaacgt tgccaatatc acgaaacgat tggttatagc
      901 tgaagatacg tccggcgatt tggttgctcg agttataaac taacagtgtc tgtacggcgg
      961 ggagtagtgc accatcggcg gcaccgagca aaaaacgtaa aatcccaagt tgcaatggcg
     1021 tctgaacgta agacattggg atcaacagca gtacagaaaa gatcagcgcc gtaatcagga
     1081 tcttttcggg tccgattcga tcgccaagtt tgccgagtcg tggtgcactt agcagagccg
     1141 ccacgcctgg caccgaggcg atcatgccac tgataaaggc gacgttactg acgttacccg
     1201 ccagttcgcg gacatacagc gtcagaatgg gggcaattga gcccgtcgcc acctggatga
     1261 ttaacgtagt gacaaacagg ctgagtacca gtttcgggtt tttaagtgat gtcaccactt
     1321 cccgcatgtg cagcatctct tttttgctga tcggctggaa tttttctctg atgcaaaaca
     1381 gggtgacgaa aaagcagagt atgagcacac tggcggtaat aaagaatacc ggacgtaagc
     1441 cgtagctatc ggcgagcagg ccgccagcca ttgggccgag caacgcacca ctaacgccgc
     1501 ctgtggagag cgtacccagc gcccagccgc ttttattacg cggtacttgt gtggcgataa
     1561 gagcattagc gttggggaca aatccgccaa gtaacccaag aagcgcccgc aggatcaaaa
     1621 actgccagat attttgtgcc agccccatca acaccatcac gatgcccatg ccgagggcag
     1681 agcgtaatag catgagtttt cggcctttac ggtcggcgag tccaccccaa aacggtgagg
     1741 cgatggccga aaataaaaat gtaatgctca tatatgctat cttaatgcac tacctgataa
     1801 gtgagcctat acaggttatt actgtatgtt tatacagtat tattggtaga agactgtgta
     1861 gatttggtat agagatgaat agatactatt aaggtaactt attgaaatca ataaagattc
     1921 ttgtgcagtc tgaatagata acttagcgtt atgtctagtc gtttccctct ccattcctaa
     1981 aaagtgctgt atactggcct atacaacgct ataaagacct ctacatagcg ccttgatagc
     2041 tatgtcacag gtacaaaaaa gccccctata aaggaggctg tgtagtctat taagggtctt
     2101 acagggtctt ggtccagtgt ctgctaacag tccttacaga gaggttaaga accttagcta
     2161 cctgagactg agatagtccc ctttccttgt actctaagac cgctctagtt gtgtcactcg
     2221 ctataggtct tccaagtacc ttaccttgaa ctctagcacg ctctagaccc tgtttagtgc
     2281 gctctctgat acggtttctt tcaaactctg caaaggtact aaatagctgt agaaccaact
     2341 taccctctga gcttgaaagg tctttaacgg gtaaatctaa acagatgagc ttcacaccct
     2401 tatcattaag aagggtcact gtattctgta ggtcgatgtt atcccttccc agacggtcaa
     2461 gtttaagaac aacaagggtg tcaccttctt ctagcttatg gttgaccaga ttcgcaaatc
     2521 cgtccctatc catagctgac actgaaccag agacaacctc ctctataact cgtgacttag
     2581 gaatatcata gccagccttc ttaatggctt gaatctggtt ctctgtggtg gaagtctcta
     2641 ctgatacacg gcagtaagca aaggttcgtg acatagatat ccctctgggt agtcattaaa
     2701 cggttagttt gtcttgtcta tagacattaa agcaaatggt aggtttgttg tctatagcta
     2761 tagtgactct tctatgtaga tattaaacgg acgtttcttg tcgtgggtca catagatact
     2821 tttagggctt ctgtcaagtg atattcgtag attttgcaag ggggaggtgt aaggctgaaa
     2881 cagggctact aagaacacat acatctaagg ctggaaagta cctgagcaga tattcttca
//
LOCUS       DAZPDU010000310         2917 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZPDU010000310 DAZPDU010000000
VERSION     DAZPDU010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.314"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..493
                     /locus_tag="ACHURS_004471"
     CDS             <1..493
                     /locus_tag="ACHURS_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFR1240029.1"
                     /translation="ADPDYVIWAKEVQGINRAWTFRHWKGTGTVGVMVATNDPERPAP
                     DESVINAVRDHILPLAPVAGSGLYVFGATEKVIPMTIALSKDTPQIRTAIKAELNALM
                     LRDGVPEGRMYLSRISEAISLSAGEVAHRLIVPSSDIDLGETELPVLGEITWQAYDPA
                     RSK"
     gene            493..1083
                     /locus_tag="ACHURS_004472"
     CDS             493..1083
                     /locus_tag="ACHURS_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFR1240030.1"
                     /translation="MDSLQDDYTKLLYGLMPPGPAWSDTDGVLDGLAPSLVRVHQRAD
                     ELVIEIDPGQSTELIERYEELYGLPDSCSPVGTQTLRQRQQRLEAKANVAGGINEQFF
                     LDQLEALGYTGVTIEQFQHLDASPDPEWGDRWRYFWRVTLPMDAGAQWQTCTDACNTP
                     IRTWGDTVAECVINKLCPSHTVVLFSYPDEDEDAQD"
     gene            1832..2110
                     /locus_tag="ACHURS_004473"
                     /pseudo
     CDS             1832..2110
                     /locus_tag="ACHURS_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            2170..2658
                     /locus_tag="ACHURS_004474"
     CDS             2170..2658
                     /locus_tag="ACHURS_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR1240031.1"
                     /translation="MTKQYDVTWLFSEDGKNWYEEQKNFASDTIKMVYTGDGRVVWVG
                     KDVTGIEPRNASVIEVPDITANRRITAPGYWFYRNDEFVFDYRLKAEDERDALLAQVS
                     ARTGEWEEDLLLGLISDEDREKLKAYRIYAKSLQAMDFSTITDKSSYNAIEWSVSPEG
                     SS"
     gene            2682..>2917
                     /locus_tag="ACHURS_004475"
     CDS             2682..>2917
                     /locus_tag="ACHURS_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR1240032.1"
                     /translation="MSVVISGALTDGAGIPMSGCQIILKSRVNTSEVVMRTRADVVTG
                     NNGEYSFEAQVGKYCVYLKRDWRDEYCVGDIAVY"
BASE COUNT          758 a          636 c          831 g          692 t
ORIGIN      
        1 cgcagaccct gattatgtta tctgggcgaa agaagttcag ggtattaacc gtgcatggac
       61 tttcagacac tggaaaggca ctggaacggt tggcgtgatg gtggcgacaa acgatccgga
      121 acgcccggcc ccggatgaaa gcgtgattaa cgcagtcagg gaccacatcc ttcctctggc
      181 acctgttgcc ggaagcggat tgtatgtatt cggtgccaca gaaaaagtca tcccgatgac
      241 gattgcatta tcgaaagaca caccgcagat caggactgca ataaaagcag agctgaatgc
      301 actgatgtta cgggatggtg tgccagaggg gcgtatgtat ctgtccagaa tcagtgaggc
      361 catcagtttg tctgctggtg aagtggccca ccggttaatc gtcccttcat cggatattga
      421 cctgggggag accgagcttc cggtgctcgg cgagatcacc tggcaggctt atgacccggc
      481 aaggagtaaa taatggattc gttacaggat gattatacaa agctgctgta cggactgatg
      541 ccgccggggc ctgcatggtc agataccgac ggtgtacttg acggtctggc accatcgctt
      601 gtgcgtgttc accagcgggc tgatgaactg gtgattgaaa tcgatcccgg tcagtccaca
      661 gagctgattg aacgttatga agaattgtat ggtttacctg attcctgttc ccctgtcgga
      721 acccagacat tacgccagcg tcagcaacgt cttgaagcaa aagccaatgt ggctggtggc
      781 ataaatgagc agttcttcct ggatcagctt gaggcgctgg gatataccgg cgtaacgatc
      841 gaacagttcc agcacttgga tgcaagcccc gatccggaat ggggagatcg ctggcgttat
      901 ttctggcgtg tgacgttgcc gatggatgcc ggtgctcagt ggcagacatg cacggacgcc
      961 tgcaacacac cgatccggac gtggggggat acggttgcgg agtgcgtgat taataaatta
     1021 tgtccgtcac acaccgtcgt tttattttcc tatccagatg aggatgaaga tgcacaggat
     1081 tgatacgctg actgcggtaa aagataagtt tggtccgggt aagaacggat ttactgacgg
     1141 aaatcttcgc acaggacgtc ttgctacctg gctgaacagt gccatgtgga atgccattca
     1201 ggaggaaatc tgtggtgtca ttgagaaggc cgggatagaa ctgaataaag aagaacacga
     1261 ccagctatat aaggctatat tattgctggt gggcggtgca attaacgaag aggcattgct
     1321 gataaaaaat aacctttcgg atgtggaaga cagggatgag gctgttgaaa acctcggatt
     1381 aaaacccacg gtggataagg caaaaaatgc cgttcagcgt gatggcgaca ccatgaccgg
     1441 agagctgaaa atccgtggtg ttaatgcgct gaggattttc aacgaagctt ttggcctgat
     1501 ttttcgtcgt tcggaagagt gcctgcacct tattcccacc agtgaaggtc agggggaaaa
     1561 tggcgatatt ggtcccctgc gcccgttcac cattaatctg cggacgggtg aaatatccat
     1621 gtcgcataaa gtgtctgttg gcggtggttc gcaggtcaat ggtgcgctgg gtatcggcgt
     1681 tcagaacgcc ctggggggga attcaattgt tcttggtgat aatgacaccg gattcaaaca
     1741 gaatggtgat ggcctgctgg atgtttatgc caatagcgtg catgtgttgc gttttcagag
     1801 tggcagtatc cagagtaata aagctgtaaa cgttacagga cgggtaacac cgtcagacta
     1861 cggaaacttt gatgcccgtt accagcagcg aaatggtggc gtgcaggatg tgcggtatgg
     1921 ttatgaaatg tattacaccc ccggcagtaa caccgtttcg tggacatttc gctcaccttc
     1981 gggacacggg ctgtcaggga tatcgatatc ggataccggc cgtaactcag cggataacgt
     2041 caacggcgtg tattaccgac cgctgcaaaa actgattaat ggcacctggt ataacgtagc
     2101 gagtatttaa caatgttgca tttaaaaaat attacggcag gtaatccgaa aaccgcggaa
     2161 taatatcagc tgacaaaaca gtatgatgtc acctggcttt tttcggaaga cggcaaaaac
     2221 tggtatgaag aacagaagaa ctttgccagt gacaccataa aaatggttta caccggagac
     2281 gggcgcgtgg tgtgggtcgg taaggatgtg acaggcattg aaccccgtaa cgccagtgtt
     2341 attgaagttc ctgatattac cgccaaccgc cgcattaccg cgccgggtta ctggttttac
     2401 cgcaatgatg aatttgtctt tgactacaga ctcaaagcgg aagatgagcg tgatgccctt
     2461 ctggctcagg tcagtgcccg gacaggggaa tgggaagaag acctgctgct ggggctaatc
     2521 agcgacgaag accgggaaaa actgaaagcg taccgtattt acgcgaaatc gctgcaggcg
     2581 atggatttca gcaccatcac tgataaatcc tcatataacg ccattgaatg gtccgtctct
     2641 ccggaaggtt cttcctgatt taatttatcg cgagaaaaac tatgtctgta gtgatatcag
     2701 gtgcgctgac tgatggcgca ggcatcccca tgtccggatg ccagattatt ctgaaatccc
     2761 gtgtaaacac ctcagaagtg gtgatgcgta ccagggctga tgtagtgacc ggaaataacg
     2821 gtgaatattc gtttgaggca caggtcggaa aatattgcgt gtatctgaaa cgggactggc
     2881 gcgacgagta ctgtgttggc gacattgctg tatacga
//
LOCUS       DAZPDU010000311         2903 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZPDU010000311 DAZPDU010000000
VERSION     DAZPDU010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.315"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..1199)
                     /locus_tag="ACHURS_004476"
     CDS             complement(<1..1199)
                     /locus_tag="ACHURS_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFR1240033.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSA"
     gene            1492..1586
                     /locus_tag="ACHURS_004477"
     tRNA            1492..1586
                     /locus_tag="ACHURS_004477"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1526..1528,aa:Sec,seq:tca)
     gene            1858..2903
                     /locus_tag="ACHURS_004478"
                     /pseudo
     CDS             1858..2903
                     /locus_tag="ACHURS_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          845 a          692 c          665 g          701 t
ORIGIN      
        1 gcgctgttct gcgctttttc tgccgcatca tatccgccat aagccagcat ccagccgata
       61 agagcgccgc cgaaggccaa ccccagtttg agcacaaaca gcgtgccagc aaaactgatc
      121 ccggtcaggc gtttaccatt gcaccactcg ccgtagtcga cggtatcgga catcattacc
      181 cactggatag gtgtcaccag ttgatgcaac acaccaatca cgaagatgaa gacaaacata
      241 gtgatgctgg cctgcatggg aacaaagaac atcgcgaggc taatcactgc cagcagggcg
      301 ttcgtccacc agaagatagt gactttacat ttccagtcgg tcagaggttt tgccagtgcg
      361 gaaccaatca ggttacccac gcaataagtg gtgagaaaag cgacaaacac ttccggcgtg
      421 cccaaaatcc atgtgacgta atacatcatc gccccaccgc gtacgcacac cgccaggata
      481 ttgaaaatgg ttagtaaacc gacaatccgc cactggtcgt tttgccagat atcacgtaaa
      541 tcttcccgca tagacgttgt tgtaggtggt gcttcaacgc gttctttagt ggtgaagaaa
      601 caaaatgcca gcatcatgaa tgccaccacg gaaaggaccg cgataccgcc ctggaaaccg
      661 agtggtttat tatcaccgcc aattaaatta accagtggca tcatcagaac agtagaaagc
      721 atgcctcccg cggtcgccag cacaaaacgc caggattgca gcgagatacg ctgagtcggg
      781 tcattggtga ttacaccacc caatgcgcag taagggatat tgacgacggt ataaagtaag
      841 gtaagtaggg tgtaagtaat tgctgcatag atcattttgc cgttcatact gagatctggc
      901 gtgctatagg ccagtacaca gacgatcccg aatggcagtg cgccaaacag tacccacgga
      961 cgaaatttac cccagcgaga gcgcgttcga tcggccaaca accccatgca aggatcggaa
     1021 atcgcatcca gtgcacgagc gaccaaaaac atggttccga caaatccggc aggaatgcca
     1081 aaaatatcgg tataaaagaa catcatatat aacattacgt tatcgaaaat aatgtggctg
     1141 gcggcgtctc ccatgccata accaattttc tctttaacgg acaacacttc actcttcatc
     1201 tgcttttcct tgatgctata ggggtgctga gaccgataac gcattttgta aaccatccct
     1261 gtgagagcga atattccgat atctggttat taatttatga ttcttgtttt atgtgatcgt
     1321 ggtagcgtta attccgctca tatatcattg taaaatatgg gttttatatg agttataatg
     1381 ctttcgtgat aatacgctgc gtgtattagg cggaaaaaac tgatctgggg gatgtagaaa
     1441 ctcaaggaag tagctataat gcgccccgcc tccatgtagc aatcgaggcg cggaagatcg
     1501 tcgtctccgg tgaggcggct ggacttcaaa tccagttggg gccgccagcg gtcccgggca
     1561 ggttcgactc ctgtgatctt ccgccagttt acctcttctg aactctccca atgtcaataa
     1621 atctcttcta atcccttgta tatcagtatt tatttgtatc ctgaggtcaa cctacttcac
     1681 cccatatcaa gtttcttttg ggggtacttt agggggtgtt atttcctatt gaaatacaag
     1741 cacccccata ataggaaaaa tgtgatacat aacaacggct tttaacaata tctttgttat
     1801 acaaggggtg tattgggggt atcaggttag ttactggcag aaatccccct caggaacatg
     1861 aattcatcaa aagccggagg atgtcatggc attaaccgat atcaaagtga aaaccgcaaa
     1921 acccaaagac aaaccctata agcttgctga tggcggcggt atgtatctac tgattaacac
     1981 taatggttct aaatactggc gcatgaagta ccgctttgct ggtaaagaaa agatgctttc
     2041 tattggcgta taccccgatg taacgctagc cgatgctcgc gagaaacgca gtgaagccag
     2101 gaaactcctc gctgctggag gcgatccagg ggaggcaaaa aaggaagaaa aaatcgcgca
     2161 gcaaatgagt ctgaaaaata cgttcgaggc tattgctcgc gaatggcacc agtcaaaagc
     2221 tgatcggtgg tcattacgct atcgtgatga aatcattgat acctttgaaa aagacatttt
     2281 tccctacatt ggcaagcgcc ctattgccga aatcaaacca atggagttac tggaagcact
     2341 gcgtaaaatg gaaaaacgtg gagctctgga gaaaatgcgc aaggtccgcc agcgctgtgg
     2401 tgaggtgttc cgctatgcaa ttgttactgg ccgggcagac tataacccag cccccgatct
     2461 tgccagtgct ttagctacac cgaaaaaagt acattttccc tttcttactg ctaatgaact
     2521 tccccacttc ctcaacgatc tggcgggtta taccggaagt ataatcacta agacagctac
     2581 tcagatcatt atgttaaccg gcgtacgaac acaagaactg cgttttgcac gctgggaaga
     2641 tatcgatttt gagacaaaat tgtgggagat cccggcagaa gtaatgaaga tgaagcgacc
     2701 tcatattgtg ccgctctctg agcaggtcat tatgctattt aagcagcttg aaccaatctc
     2761 aaaacatcat cccctagtct ttatcggtag aaatgatccc cgcaaaccaa ttagtaagga
     2821 aagcattaac caagtaattg aattacttgg atataaggga agactcacag ggcacggttt
     2881 cagacacacc atgagtacaa ttc
//
LOCUS       DAZPDU010000312         2878 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZPDU010000312 DAZPDU010000000
VERSION     DAZPDU010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.316"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..954)
                     /gene="curA"
                     /locus_tag="ACHURS_004479"
     CDS             complement(<1..954)
                     /gene="curA"
                     /locus_tag="ACHURS_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFR1240034.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDGL"
     gene            1135..1653
                     /gene="mddA"
                     /locus_tag="ACHURS_004480"
     CDS             1135..1653
                     /gene="mddA"
                     /locus_tag="ACHURS_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFR1240035.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHHSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            1650..2099
                     /locus_tag="ACHURS_004481"
     CDS             1650..2099
                     /locus_tag="ACHURS_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR1240036.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(2100..2333)
                     /gene="ydcY"
                     /locus_tag="ACHURS_004482"
     CDS             complement(2100..2333)
                     /gene="ydcY"
                     /locus_tag="ACHURS_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFR1240037.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(2419..2592)
                     /gene="ortT"
                     /locus_tag="ACHURS_004483"
     CDS             complement(2419..2592)
                     /gene="ortT"
                     /locus_tag="ACHURS_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFR1240038.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            2787..>2878
                     /gene="yncL"
                     /locus_tag="ACHURS_004484"
     CDS             2787..>2878
                     /gene="yncL"
                     /locus_tag="ACHURS_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFR1240039.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWF"
BASE COUNT          691 a          738 c          692 g          757 t
ORIGIN      
        1 taaaccatca gtaatatctt cgtggtagtg gattttatcc tctttcaccc attgccccat
       61 ctccttctga aactcatgga tgcggtgacc ataatcctga gcgataataa acccttgcaa
      121 gcgaatacgt tttttcagca ccgttgccat caacaggggt aaacgatccg gaccgggtgg
      181 tagctctgta gcgttatagc tgctcactaa gccacaaacg ggaatgcgcg cagatgtatt
      241 aagtaatggt agcactgcat caaatacctt accgcccacg ttttcataat agatatcaat
      301 accttttggg cacgctttca ccagttgttc ggcaaaatca tccgcgtggt gatcaagaca
      361 aacatcgaag cccagcacct cgatagcatg gcggcatttt tccgcgccac cggcaacccc
      421 caccaccctg caacctttaa gtttgccgat ttgccccacc gttgctccca caggacctgt
      481 cgccgcagct accaccaacg tttcgccctc tttaggctga ccgatatcca gcagccccat
      541 ataagcggta aagcctggca tccccagcac acccagcgac cacgatggat tttgcggatg
      601 atcgccaagt ttcaccagat catcaccact ggatatgtca tagtcctgcc atccactgta
      661 gcccagcacc cagtcgccag gctgataatc aggatgattc gactccacaa cacggctcac
      721 cgtgccgccg accatcacgc cgccaatatc aacaggcggt gaataagatg gctcatcgct
      781 catacgtcca cgcatatacg ggtccaggga caaataaact gtgcgcagta acacctgtcc
      841 ttcacccggt gtggcgacat catcttcttc aagacggaaa ttctccggaa ccggtgcgcc
      901 atgtggacgc gaggccagaa cccaacgtcg attacgctgc ttttgttgcc ccataatgct
      961 ctcctgtgct tttggcatga atccatcaat agtagcaggc tcacgggatt ggttttgttt
     1021 gcagccgtca catcccctct gcaacacgca ataatttcat cgaggaatcc gttgcttcgg
     1081 gctgttttcg atagtattga taacatcatc atcattattt tatcgaggtc agacatgtcc
     1141 atccgttttg cccgcaaagc cgattgtgct gccattgcgg aaatttataa ccacgccgtg
     1201 ttgtatacgg cggctatctg gaatgaccaa acggttgatg cagataaccg cattgcctgg
     1261 tttgaagcgc ggaccatagc aggttatccg gtgctggtaa gtgaagaaga tggcgtagtg
     1321 acgggatatg cctcgtttgg cgactggcgt agtttcgatg gttttcgcca taccgtggaa
     1381 cattcggttt atgtccatcc cgatcatcag ggcaaaggtc tggggcgtaa attgttaagc
     1441 cgattgattg atgaagcgcg ggattgcggg aagcatgtca tggtcgccgg gatcgaatcg
     1501 caaaatcagg cctcgctgca tctgcaccat tcgctgggat ttgtcgtcac cgcacaaatg
     1561 ccgcaggtgg gcactaaatt tgggcgctgg ctggatctga catttatgca gttgcaactc
     1621 gacgaacgca ctgaaccgga cgcgattgga tgaatcagtc gctcaccctt gcttttttga
     1681 ttgccgccgg aattggtctg gtagtccaga acacgctgat ggtgcgcatc acccagacct
     1741 cctctaccat tctcatcgcc atgttgctga actcactggt ggggattgtg ctctttgttt
     1801 ctattttatg gtttaaacaa ggcatggcgg ggtttggcga actggtgtcc agcgtgcgct
     1861 ggtggacact tatacccggc ttgctgggat cgttttttgt cttcgccagt atcagcggat
     1921 atcagaatgt gggagcggcg acaactattg cagtactggt cgccagccag ctaattggcg
     1981 gtctgatgct ggatatcttc cgcagtcatg gcgtaccact acgggcgttg ttcggcccaa
     2041 tttgtggcgc aattttactt gtggttggcg cctggttggt agccagacgc tcattttaat
     2101 cagaggatgg tgccgccttt ggttaactgt tcgtggcgcg cttccatatc ttctttatgc
     2161 ttgcgaccgt gatgagcaat agcggtgcgc aaacgctgct gctgggtgta gcgatcttcc
     2221 cgacttaact ccgcgtcatc gctcagggcg attaataatt cgttcatatg ggcaatgacg
     2281 ctctcttcaa tcgcggcatc cacgcgcgcg atgacttcat ccagatgcga catactttct
     2341 ccttaaccac ttgtgaaatt tatcgattca tccaaatgat ataagtctgc gtggtgccgt
     2401 caagccagtg agtaagattt aaaacagtga aaacaacagc gccaccggga aactcatcgg
     2461 ccatgttgcc cccaccagaa aggcgcttaa gaaacggatg cgtttcttat cgtgagaaag
     2521 aaaccaggtg ataagaaatg cgattgctgc cataaccgca taaaaaacaa gcatatgttg
     2581 atagagagac atgtacgggc gtccagccag atgaataaaa cgcgcgcaaa tatgcgtcat
     2641 tttttgacgt gcatcaagtt ttttcaccag tttgagaaaa aatcatcaaa cagcaacttg
     2701 cccttatcca tcttcagaat gagtattatt gcggattttc ttaacccgta ctatacacat
     2761 aggggcaatg ataaaaggtg gcaaaaatga atgtttccag tagaactgta gtactgataa
     2821 atgtctttgc tgctgttggt ttgtttactc ttatctctat gagatttggc tggtttat
//
LOCUS       DAZPDU010000313         2818 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZPDU010000313 DAZPDU010000000
VERSION     DAZPDU010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.317"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..782
                     /locus_tag="ACHURS_004485"
     CDS             <1..782
                     /locus_tag="ACHURS_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFR1240040.1"
                     /translation="SVSAPGHHSWKRDFDPDSPEIKFSDRREALKLMTQIESTVTSLH
                     REAEAQFRPELEKIVRGIETGFRGTALYATENIAGRINARLEDEGFTVKITFPAVSQL
                     QTRIAVKTNLSALMEERTETVTRRRRQSGFWGKICGAFGTSDWGWENYKENVSRSVIN
                     INSVRKEVMSLTRAYFGELQASIEQNINQPVRQEIDDFFCTFREKVEQLRNTLIQSSE
                     DHKRDQQAQEQLTERLQALNERVPELITDSKALREELETLL"
     gene            779..1684
                     /locus_tag="ACHURS_004486"
     CDS             779..1684
                     /locus_tag="ACHURS_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFR1240041.1"
                     /translation="MTSPFIQQIADNRVCQVLSCLPEKFVVDFANGIDVAQEHNRTAG
                     GRTFFRRLKEGLTGKGAVRQNAINASLAQGVEASLRWLTELTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLAEQVHQKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     NGGSATVRLNTLDWLGGQSREQADNEWHEAVNWLGDWCSEERHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGEA"
     gene            1681..2751
                     /locus_tag="ACHURS_004487"
     CDS             1681..2751
                     /locus_tag="ACHURS_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFR1240042.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGAQISMVSGASIG
                     ALNGAIITASPDLSEAALRLEALWDHLGNNQVLSVNRSVYFSLLKKLVQAMNLCQIPG
                     RAGALLTTLFRHISTINGFDNPMIQPLLSDEPLTALMDHYLDTDALADGLPLYVSLYP
                     TEGGMQDIIDCIRAELGAGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMYGDGGMGGWRNRQGNTPVTPLVDAGCNMVIVTHLSDGSLWDRRAYPDTTILEIRPR
                     KRLKQIGDEGKSGGLLSFTSAHTDAWRQQGYEDTMLTMEHIRKPLAARQALTRSETVL
                     QKSLEITEGADSALRNAMARIK"
BASE COUNT          707 a          675 c          824 g          612 t
ORIGIN      
        1 ttagtgtcag tgccccgggc catcattcat ggaaacgaga ctttgatccg gacagccccg
       61 agataaaatt cagtgatcgc agggaagccc ttaaactgat gacgcaaatc gaatcgaccg
      121 tgaccagcct gcaccgtgag gctgaagcac agttccggcc tgaactggaa aaaatcgtca
      181 ggggtatcga aacaggtttt cgtggtacgg ccctgtatgc cacagaaaat attgccggac
      241 gtatcaatgc ccgcctggag gacgagggct tcactgtaaa aatcactttc ccggcagtca
      301 gccagttaca gaccaggatc gcggtaaaaa caaatctgag tgcgcttatg gaggaaagaa
      361 ctgagacagt cacccgtcgc cgtcggcaga gtggtttctg gggaaagatt tgtggagcgt
      421 ttggcaccag tgactggggc tgggaaaact ataaggagaa tgtgagccgc agtgtgatca
      481 atatcaactc ggtcaggaag gaagtgatgt cactgacccg ggcatatttc ggggagttgc
      541 aggcatccat tgagcagaat attaaccagc ccgtccgcca ggagatcgat gattttttct
      601 gtacattcag ggaaaaagtc gaacagctac gtaacacact cattcagagc tctgaagatc
      661 ataaacgcga tcagcaggca caggagcagc ttacggagcg ccttcaggca ttaaatgaaa
      721 gggttcccga gctgattact gacagtaagg cgttaaggga agaactggag acactgctgt
      781 gacctcacca tttattcaac aaattgctga taaccgggta tgtcaggtac tcagttgcct
      841 tcctgaaaaa tttgtggttg attttgcaaa cggtattgat gttgcacagg agcataaccg
      901 cacggccggg gggcgcacgt ttttccggcg tttaaaagaa ggtctgactg gcaaaggcgc
      961 tgtacgccag aatgccatca atgcttcgct tgcacagggc gttgaggcgt ccctgcgctg
     1021 gctgacggag ctgacaacgt cgcttgccac cacaaattac gcgattaccc gggtaaacga
     1081 cagggtcagt tcactggtca gtgatactgc caggctggcg cattattcgg cagacacgcg
     1141 ggagcagtta ctcaccctgg cagaacaggt tcatcagaaa ctgaatcatc ttgaagaaaa
     1201 actccaccgt gttgaccagg ttcagcgggc acagttacat cttgagcaga tattctcatg
     1261 gtggagcgcc gggcgatacg cgtcattttc ccctgccgga cgttgttatg tggcgcttga
     1321 agagcttcgc tggggcgcgt ttggtgatgt gatacgtcag ggtgaaacag gccaggttaa
     1381 ccagttactg gatatcctca gacataaagc attaacgcaa atggcacagg agaatggcgg
     1441 tagcgcaaca gtacgtctga acacactgga ctggcttggt ggtcagagcc gggaacaggc
     1501 tgataacgaa tggcatgaag cggttaactg gctgggggac tggtgcagtg aagagcggca
     1561 tcctgtgatc tggtcaacca cacaggctgc agaacactta ccggttcgta tgccccgtct
     1621 ctgctctgca gaacgcctct ctgaaagtat ggttgatgaa atatttcaga agggggaggc
     1681 atgagtacag aaatgaaaac ggggctggtg ctgtccggcg gcggggcggt gggcgcttat
     1741 caggcgggag tggttaaggc actggcagag tgcggcgcac agatcagtat ggtctcagga
     1801 gccagtatcg gggctctcaa tggtgccatt atcacggcct ctcccgatct gtcagaagcc
     1861 gccttacgcc tggaggcgct ctgggatcat ctggggaata atcaggttct gtcggtaaac
     1921 agatcggttt acttttcatt gctgaaaaaa ctggttcagg ccatgaacct ctgccagatc
     1981 cccggacgtg cgggcgcact gcttacgacg ctttttcgcc atatatcgac aatcaacggg
     2041 ttcgacaatc cgatgattca gccgctgttg tcagatgagc ccctgacagc gctgatggat
     2101 cattatcttg atacagatgc tctggccgac gggctaccgc tgtacgtgtc gctgtacccc
     2161 acagaaggag gcatgcagga tattattgac tgcattcgtg ctgaactggg tgccggaacc
     2221 acgaaaaacg ctgtttttca gcatatccag agtctgcccc gcgggcagca gaaagaagcc
     2281 ttactggcgt cagccgcgct gcctctgctg ttccgtcccc gcgaggttca gggaacaatg
     2341 tacggtgatg gtgggatggg cggatggcga aacaggcagg ggaatactcc cgtgacgcca
     2401 ctggtggatg ccggatgcaa tatggtgatt gtgacgcatc tgagtgacgg ttctttatgg
     2461 gatcgtcggg cttatccgga caccacaatc cttgaaatcc gtccccggaa aaggttgaaa
     2521 caaatcgggg acgaaggcaa aagtggcggt ctgctcagtt ttacatcggc acataccgac
     2581 gcctggcgtc agcagggcta cgaggacacg atgctgacga tggagcatat ccggaaaccg
     2641 ctggcagcac gtcaggcact gacccggtca gagacggtat tgcagaaaag cctggagata
     2701 actgaaggtg cagattcggc actgagaaac gcgatggccc ggattaaata aagacgctcc
     2761 ggagaaacgc caccgccact gtggtggtgt ttttcctgtc tggtattatt gaaaatat
//
LOCUS       DAZPDU010000314         2790 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZPDU010000314 DAZPDU010000000
VERSION     DAZPDU010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.318"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..499
                     /locus_tag="ACHURS_004488"
     CDS             <1..499
                     /locus_tag="ACHURS_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFR1240043.1"
                     /translation="WKRIPKFWNWIPASKIEKETRMYGTCETLCRALAAKYPGDTPLM
                     LVIWSPEEIQALADGMDISLSDHEIRTVLARLEDIPEDQRTESGISSGAAMEIINNVR
                     ENRQVTVPAELLASLIQTAEQALWKREWAARDNGLAVPECVTRRQAVVNQARALLKNN
                     TREND"
     gene            544..1314
                     /locus_tag="ACHURS_004489"
     CDS             544..1314
                     /locus_tag="ACHURS_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240044.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQRNPKYPGWISIYVL
                     LDAPRLATLLINRHGGVLPPLLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVHDAVRSVSCRVAEDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSSVEMYLVSECVEHILSSGLACDVLR
                     IPDEPSRRWFDRDILREVVLEARTEIRSMADALAKIRK"
     gene            1730..2155
                     /locus_tag="ACHURS_004490"
     CDS             1730..2155
                     /locus_tag="ACHURS_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFR1240045.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGLDFLYAEASVYALAKKMMP
                     EYEGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTHLYRMRDAQLWSHIEFHPECNAIYAALD"
     gene            2202..2624
                     /locus_tag="ACHURS_004491"
     CDS             2202..2624
                     /locus_tag="ACHURS_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFR1240046.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSECVFAVVLTRGDVRHIAQDWNLSD
                     DELETVMQRLDDAFVYGACDRVVSDIVNELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREELEAMDVVLQALDGETMS"
BASE COUNT          591 a          744 c          870 g          585 t
ORIGIN      
        1 atggaagcgt ataccgaaat tctggaactg gattcctgct tcaaagattg agaaggagac
       61 gagaatgtac ggaacatgcg aaacgctctg ccgggcgctg gcagcaaagt atccgggaga
      121 tacgccgctg atgctggtta tctggtcccc ggaagaaatt caggccctcg ctgacggaat
      181 ggatatttcc ctgtccgatc atgaaatcag gactgtcctg gcgcgcctgg aggacatccc
      241 ggaagaccag cggactgaat ccggtatttc ttccggtgcg gcgatggaga tcatcaataa
      301 cgtgagagaa aaccgccagg tgaccgtccc tgctgaactg ctggcgtccc tgattcagac
      361 cgccgaacag gcactgtgga aacgtgagtg ggccgcccgt gataacggcc tcgccgtccc
      421 ggaatgcgtc acccgccgac aggcggtggt taatcaggcc cgcgccctgc tgaaaaacaa
      481 cacacgcgaa aacgactgat gttatcgccg cctgcgggcg gcgattcagg gagaccatga
      541 atcatgaacg aaacgctgaa tgcactgatt tgtcgccacg cccgtaacct gctgctggcg
      601 cagggctggc cggaagagac ggatgttgac cagcgtaacc cgaagtatcc gggctggatc
      661 agcatttatg ttctgctgga tgcgccccgg ctggcgacgt tacttatcaa ccgtcacggc
      721 ggcgtactgc cgccgctcct ggcctctgcc attcagaaac tgaccggaac cggggcggaa
      781 cttgttctgt ccggcagtca gtggcagtcg ctgccggtac ttccggcaga cggaacgcag
      841 gtgtctttcc cgtatgccgg agaatggctg accgaagacg aaatcagggc agttcttgat
      901 gcggtgcacg atgcggtacg cagtgtcagt tgccgggtgg cagaagatgc gcggcgtatc
      961 agggcggcgc tgaccacaac cggtcagacg ttgctgaccc gccagacgcg ccgctttcgc
     1021 ctggttgtga aggaaagcga tcacccctgc tggctcgatg aagacgacga aaacctgccc
     1081 gtggtgctcg atgccatcct gaaccggggc gctcgttttt cgtcggtgga aatgtatctg
     1141 gtcagtgagt gtgtggagca tatcctgtcc agtgggctgg cctgcgatgt gctgcgtata
     1201 cctgatgaac cgtcccgtcg ctggtttgac cgggatattc tgcgggaggt ggtcctggaa
     1261 gcccggactg aaatccgcag catggcggat gcactggcga aaatccggaa atgatgacgc
     1321 tggcggggaa aaatctccgc cttttccagc cggtccccgg aaagagcaaa aggatgtcgc
     1381 cgcaggctcc gctttacccg gccatgcggg gcatggcctt gtgggttttc agttatgtgg
     1441 cctcagcgcc gtgtgcgggc tgtgccgtgc ctccatcgta gccgggcttc tgcgggcggg
     1501 ttgcaaggta aggtgaaccg ctgcggtcac ccggtccctc cttcccgtct gccgtgattt
     1561 tccggtcgtt cacccgctca gatttcgcgg tctcaccctt caactcccgt cagccgtgcg
     1621 cgtcaggctg cggcttccct tgcgcccttg caaccctgca ggcccggcaa agatggaggc
     1681 acggcaccgc ccggtgtcgc ggaatatgtg aacaacggag gaaaacatca tgcaatatgc
     1741 gaaacctgtc actctgaacg ttgaagagtg cgaccgtctg tcttttctgc cttacctgtt
     1801 tggcctggat tttctgtatg ccgaagcgtc tgtatacgcg ctggcgaaaa aaatgatgcc
     1861 ggaatatgaa ggcggattct ggcacttcat ccgcctgccg gacggtggcg gttacatgat
     1921 gccggatggc gaccgtttcc acatggtgaa cggtgcaaac tggtttgacc gtaccgtgag
     1981 tgctgatgcc gcaggcatca tcctgacctc ccttgtgatt aaccgccagt tgtggctgta
     2041 ccacgacagc ggggatgcag ggttgacaca cctgtacagg atgcgtgatg cgcagttgtg
     2101 gagccacatc gaatttcacc ccgaatgcaa cgcgatttac gcggcacttg actgattaac
     2161 ggacggggcg gcaatgccgc ccctgtaaag gagacgagaa catgtactgt actgttaaag
     2221 aaatcatccg cgatgtactg gatacagacg tgccggacag tgaatgcgtt tttgccgtgg
     2281 tgctgacccg tggggatgtg cgtcacatag cccaggactg gaacctgtca gacgatgagc
     2341 tggaaaccgt catgcagcgg ctggacgatg cttttgtata tggcgcatgt gacagagttg
     2401 tttccgatat tgtcaatgaa ctgatggaag aaaagcgcgc cagccgtcag gtgacagtcc
     2461 cggcggtgat gctggaaaaa gtgatggcgc tggcaggcag tgaaatgaag cgcctgtatg
     2521 ccgtcgggag tgagaacggg ggcgacggtg acgcattcgt cagggaggaa ctcgaagcaa
     2581 tggacgttgt gttgcaggcg ctggacgggg agacgatgtc atgaatatca gcacagaaac
     2641 ccgcgaaatt ctgcgtaatt acagggccgt gattaatgcg cggcgtcgtg aaatggggca
     2701 gaaaccgctc accaccgcgc agattgttga tgaaatctgc gattttgtgg cgaatcagca
     2761 ggcggttttc ctgggcggtc actacatcct
//
LOCUS       DAZPDU010000315         2790 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZPDU010000315 DAZPDU010000000
VERSION     DAZPDU010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.319"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            143..727
                     /locus_tag="ACHURS_004492"
     CDS             143..727
                     /locus_tag="ACHURS_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1240047.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
     gene            832..1572
                     /locus_tag="ACHURS_004493"
     CDS             832..1572
                     /locus_tag="ACHURS_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFR1240048.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDNNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            1607..2790
                     /gene="htrE"
                     /locus_tag="ACHURS_004494"
                     /pseudo
     CDS             1607..2790
                     /gene="htrE"
                     /locus_tag="ACHURS_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
BASE COUNT          798 a          584 c          598 g          810 t
ORIGIN      
        1 gtcctaatga acaatggttg taaattttgg taacaaatat cattgctcat taatatctag
       61 catctgctta taacatttgt gtcatgaata gtcattcttc aaaccagtag tctgactaat
      121 ggaattttat tcaaggattt ttatgtctaa aaaattaggt tttgccctgt ctggtcttat
      181 gctggcaatg gttgcaggta ctgcttctgc tgatatggat ggtggtcagt taaatatcag
      241 tggtctggtt gttgataaca cctgtgaaac ccgcgttgac ggcggtaata aagatggtct
      301 gatcctgctg caaactgcaa ctgttggtga aatcgatgct ggcgtgctga atgacaccgt
      361 tggcgctaaa gccaaacctt tcagcatcac cgttgactgc agcaaagcaa atccgaatcc
      421 aggtagtaca gctaaaatga ccttcggttc tgttttcttt ggtaatagca aaggaacgct
      481 gaacaacgac atgtctatta acaacccgtc tgacggcgtg aatatcgctc tgcacaatat
      541 tgatggttcc actatcaaac aggttcaaat taacaaccct ggcgatgtgt atactaaagc
      601 cctggatgca acgacaaaat ctgctgttta tgattttaaa gcgtcttacg ttcgtgccgt
      661 cgcagaccaa acagcaactg ctggttatgt aaaaactaac actgcataca ccattactta
      721 tcagtaagta ttcaccaatt aaattgattg aatgaatata cagggaatag tgttaataat
      781 ttctattttt atattattcc ctgttttaat taactctgtc agggatggtt tatgtttttt
      841 aacaccaaac atacaacagc tttatgcttc gtaacctgta tggcttttag ttcatcgtct
      901 attgcggaca ttgtcatttc gggtactcgc gtaatatata aaagcgatca aaaaagtgtc
      961 aacgtacgtc tggaaaataa agggaataac ccgttgcttg tccagagttg gttagatact
     1021 ggcgataaca acgctgagcc tggcagtatt acagtccctt ttactgccac gccgccagta
     1081 tcgcgtattg atgccaaacg tgggcaaaca atcaaattaa tgtacacagc cagcacctca
     1141 ctgcctaaag acagagagag cgtgttctgg tttaacgtac tggaagttcc accaaaacca
     1201 gatgcagaaa aggtcgcgaa tcaaagtctg ctgcaactgg catttcgcac acgtataaaa
     1261 cttttctatc gcccggatgg attgaaggga aatccctctg aagccccgtt agccctgaag
     1321 tggttctggt caggttcaga aggtaaggcg tcattgcgag tgaccaatcc aaccccttac
     1381 tacgtctctt ttagcagtgg tgatttagaa gctagcggta aacgctatcc gattgatgtg
     1441 aaaatgattg caccatttag tgatgaggtc atgaaagtca ctggccttaa tggcaaagca
     1501 agttctgcaa aagtacattt ttacgccatt aatgattttg gtggcgccat tgaaggtaat
     1561 gccagtctgt aatcaggcaa ggatataatt ccgcaggaag cataacgtga ctataaaacc
     1621 aactaaaaat tatcataatc atctgacccg tatcgctacg ttttgcgcgc tgctgtattg
     1681 caatagtgct ttctgtgctg aacttgttga atatgaccat accttcctga tggggcagaa
     1741 tgcctcaaat attgatctca gccggtatag tgaaggtaac cctgctatac cgggtatgta
     1801 cgacgtcagt gtttatgtca acgatcaacc tattattaac caaagcatta catttattga
     1861 aattgaagga aaaaaaacgc acaagcctgt atcacactta agaatttatt gcagtttcat
     1921 ataaattctc ccgatataaa taacgaaaaa gccgttctgc ttgccaggga tgaaacactc
     1981 ggcaattgcc ttaatttgac ggaaattatc cctcaggctt ctgttcgtta tgacgttaac
     2041 gagcaacgtc tggatataga cgttcctcag gcatgggtaa tgaaaaatta ccaaaactat
     2101 gttgatccat cgttatggga aaatggcatt aatgcggcca tgttgtcata caacctcaac
     2161 ggatatcata gtgaaactcc tggtcgaaga aatgacagca tttatgctgc ttttaacggt
     2221 gggatgaatt taggtgcatg gcgactgcgt gcctcaggca actacaactg gatgaccgat
     2281 tctggcagta attatgattt taagaatcgg tatatacagc gtgatatcgc ctcgctgcgt
     2341 tctcaactca ttcttggtga gtcttataca acgggcgaaa cctttgattc tgtcagtatc
     2401 cgcggcattc gtttatacag cgacagccgc atgttgcctc cgacattagc gagttttgcg
     2461 cctatcattc atggcgttgc taataccaac gccaaagtca ctattacgca aggtggctat
     2521 aagatttatg aaacgactgt gccgccaggc gctttcgtca ttgatgatct gagtccgtca
     2581 ggttacggca gcgatcttat tgttactgtc gaagaatccg atggctcaaa gcggacattc
     2641 tcgcaacctt tttcatcggt cgttcaaatg ttacgccctg gcgttggacg ttgggatatt
     2701 agcggcggcc aggtcttaaa agatgatatt caggatgagc ctaatttatt tcaggccagc
     2761 tactactacg gtctgaataa ctatctaacg
//
LOCUS       DAZPDU010000316         2729 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZPDU010000316 DAZPDU010000000
VERSION     DAZPDU010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.320"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(423..680)
                     /locus_tag="ACHURS_004495"
     CDS             complement(423..680)
                     /locus_tag="ACHURS_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240049.1"
                     /translation="MSDHVIECASRAGRDFSEFMKGEKGMMEALASVDEFGEQLRLNG
                     CVNHHFVSYMMRNSIMQAFMDMAKAERKEERRRKRAEAKAK"
     gene            complement(677..1174)
                     /locus_tag="ACHURS_004496"
     CDS             complement(677..1174)
                     /locus_tag="ACHURS_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFR1240050.1"
                     /translation="MKSLTLFNQPIRIGEDGMICLTDMWKASGKSESESPYHYLRNKQ
                     TKEFLAELEKNHESVVFTERGVHGGTYGGKFVAYDYAAWLNPGFKYAAYKVLDDYFTG
                     ELQHRNSLSAQLNMKCHEFDQKKDMASFCGQGLAAWRYTKPVLVAEINSLANQLQITI
                     PGLPG"
     gene            complement(1376..1834)
                     /locus_tag="ACHURS_004497"
     CDS             complement(1376..1834)
                     /locus_tag="ACHURS_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR1240051.1"
                     /translation="MSRVTAIISALVICIIVCLSLAVNHYRDNAIAYKEQRDNKASEL
                     EKANATIADMRKRQRDVAELDARYIKELADANATIESLRADVSAGRKRLQVAATCAKS
                     TTGASGMGDGESPGLTADAELNYYRLRSGIDKITAQVNYLQEYIRTQCLK"
     gene            complement(1831..2328)
                     /locus_tag="ACHURS_004498"
     CDS             complement(1831..2328)
                     /locus_tag="ACHURS_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFR1240052.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPGGNDGLEGVRHNPYKD
                     IVGVWTVCYGHTGKDIIPGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRCAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGNQWKGLMTRREIEREVC
                     LWGKQ"
     gene            complement(2328..2543)
                     /gene="essD"
                     /locus_tag="ACHURS_004499"
     CDS             complement(2328..2543)
                     /gene="essD"
                     /locus_tag="ACHURS_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFR1240053.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGG"
BASE COUNT          649 a          708 c          621 g          751 t
ORIGIN      
        1 atacagcgca tgatgtgtca ggcttgaata cctttatcct ttaaaaggga tatcagttaa
       61 gttatcccgt gtagggtata agccattatc aaagccactc tgtagggaat ggcttttgtg
      121 atggcatcac ttactcttta cgctgctatc ccactcatcc cggaattttg atgggttatt
      181 gaaaccttct gctgacataa caactccttc aatgtttggc tgaaattggg atgtctttcc
      241 atcagtccgc caccacaaag aatctttttt gccataaggc aggaggttca tctttcagtg
      301 gctgccggtg ttatttcccc acttactggc ttgggttgtt tcgtggtact gccgttaact
      361 ggtggcccag aataaattcc ggtttcatta tcaagcccac ccgtaaatgg gctttgtaat
      421 ggctacttcg cttttgcttc cgctcgctta cgccggcgct cttctttcct ctcggctttt
      481 gccatgtcca tgaatgcctg catgatcgag ttccgcatca tgtagctaac aaagtgatga
      541 ttgacacagc cgttgaggcg cagctgctcg ccaaactcat ccaccgaggc caatgcttcc
      601 atcatgccct tctcgccttt catgaactct gagaagtcgc gccccgctct ggaggcgcat
      661 tcaataacat gatcactcat cccggaagcc cggggatcgt aatctgcagc tggttagcca
      721 gggagttaat ctcagcgacc aacactggct tcgtatagcg ccatgctgcc agcccttgtc
      781 cgcagaagct cgccatgtct ttcttctggt caaattcatg acatttcatg ttgagctgcg
      841 cacttaagct gttgcgatgc tgaagttctc cggtgaagta gtcatcgagg actttatagg
      901 ccgcgtactt gaacccgggg tttaaccaag ccgcataatc gtaagcaaca aacttcccgc
      961 catatgttcc accgtgtaca ccgcgctcag taaaaaccac agattcgtgg tttttctcca
     1021 gctcggctaa gaactctttg gtctgcttgt ttcgcaggta gtggtaaggc gattcagatt
     1081 cacttttacc actggctttc cacatatcag tgaggcagat catgccatct tcaccgatac
     1141 gaattggttg attgaagagg gttaatgatt tcatagcgtg tacctactct ttgaaatgaa
     1201 cctttgccgc acaggaaacc agcccaccga ggctcgccag cactaactgg tatcctcaaa
     1261 ggcccattcc aaaggggcag gttcggtgta aaaaacatgc gttgcggtac gcatttattg
     1321 caaaaagccc cgcatcgcga ggctcattaa atggactttg tgatttgcaa aaaaattatt
     1381 tcaggcattg cgtcctgatg tattcctgca ggtagttaac ctgcgcggtt atcttgtcga
     1441 ttccacttcg gagacggtaa taattgagtt cagcatctgc tgtaagtcct gggctttctc
     1501 catcgcccat gccgctggct ccggtcgttg actttgcaca ggtggcggcg acttgcaggc
     1561 gcttacgccc agcagaaaca tcagcacgga gactttcgat agtcgcgtta gcatcagcaa
     1621 gctcctttat gtatcttgcg tcgagttctg ctacatcacg ttgacgcttc cgcatgtcag
     1681 cgatggtggc gttcgccttc tccagttcac tggccttgtt atcgcgctgc tctttgtagg
     1741 cgattgcgtt atcacggtaa tgattaacag ccaatgacag gcagacgatg atgcagataa
     1801 ccagagcgga gataatcgcg gttactctgc tcattgtttc ccccacaaac agacttcacg
     1861 ctcaatctca cggcgagtca tcagtccttt ccattgatta ccgccagcgt atgtccagcg
     1921 ccgtagctga tcacatgcgc ctttaatatc accctggttt attttgcgaa gaagcgtcga
     1981 tgttctgaaa ttgccagcac ccacgttgta gacgaacgag taaagagcgc agcgcgttgt
     2041 ttccggtata tcgactttga tgtacgggtt aatttgtctg gcgacagtgg caaggtcttt
     2101 attcaggagg gctttgcact ctgcttcggt atacgtttta ccgggaatga tgtcttttcc
     2161 tgtatgcccg taacatacag tccatacacc aactatgtct ttgtaaggat tatgtctcac
     2221 accttccaga ccatcgttac cacctgggcc agtgattaac acagatgcta tagcaatagc
     2281 cccgccacca atagcagcag caacagcttt tcgtaatgat ggaggcatta tccacctctc
     2341 gcagccttgc gcttatcttc tttaatcttg aaataaaggt ttgtcaggta cgtcagcagg
     2401 ccaaatacca ggctacccag cactccaatt gccgcccact gtgagggcgt gactttatct
     2461 agcagctgta aaaaccagta cccggcacta cctgctgagg tgccataggc gacacccgtt
     2521 gttaacttat ccatggattt cataacccca cctcgcagac aaagcgggtg taaattgagg
     2581 gaatactacg aaacgtaaca gactcggagt cagtgaataa ctcaggtatt gggttatcag
     2641 ctaatatcga gactcaaaaa atggaaaaac ccgctcgacg gcgggtttaa gctgtgtgac
     2701 gaagtaacca ctcttaacag cataaccaa
//
LOCUS       DAZPDU010000317         2713 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZPDU010000317 DAZPDU010000000
VERSION     DAZPDU010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.321"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..269)
                     /locus_tag="ACHURS_004500"
     CDS             complement(<1..269)
                     /locus_tag="ACHURS_004500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240054.1"
                     /translation="MAATAASSAFAVALLPPDTPCRWRFVEADTSGFIFNDEKPGVSF
                     CLFHRWGTNAEILLSSVFACRGLKRQRFQALFRLVNILAWLFGNI"
     gene            329..697
                     /locus_tag="ACHURS_004501"
     CDS             329..697
                     /locus_tag="ACHURS_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001456887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240055.1"
                     /translation="MKDNNTTATPETWKMRFLANGTSGTWFYTDDIAVTDAWLRAHPD
                     DVEVIPLCRIQPRQHSHEQHIEELRAALARMLHMHHMMLETTHYRENITDARCIREMH
                     EAPLQALNILSRYPHKKDEV"
     gene            701..916
                     /locus_tag="ACHURS_004502"
     CDS             701..916
                     /locus_tag="ACHURS_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240056.1"
                     /translation="MWLLIFWLSSPFNVSSGDARQPTLAVHSQAFHTERACRDALRAI
                     KKENVGEFSLRGVCVSDGNTKEEAESS"
     gene            complement(1114..1737)
                     /locus_tag="ACHURS_004503"
     CDS             complement(1114..1737)
                     /locus_tag="ACHURS_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001466373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1240057.1"
                     /translation="MQEHIDYQIEKYQFRARNESPRLMRQWEDVMRECRDTGAGARER
                     LRIALLNVDYVTSFELPFRLLLTRTPQMIDSVRREWQLSQKKVVFNERQRGCVYSLQN
                     DLSGVPDSFSYSLATRIRRADSYGMTTRCFTDIAGQVKAPTARLKLALESGLIVTALD
                     GLFWLGIQRIAADVQRLRQSGMTIITRDVEVSDSLTGTTRVVSGYSL"
     gene            1897..2364
                     /locus_tag="ACHURS_004504"
     CDS             1897..2364
                     /locus_tag="ACHURS_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFR1240058.1"
                     /translation="MIKYIFSLTIIFSASLCAAQIQGHVIRVLDGDTLEILQDKKPVR
                     VRLANIDAPEKKQPFGRWSATQLKDLVAGKPVTVQYAQTDRYGRILGRVYTADGTEAN
                     RWQVRNGAAWVYPDYNNDYTLPPLQRDARALKRGLWADKNPVPPWTWRKQNKK"
     gene            2515..2691
                     /locus_tag="ACHURS_004505"
     CDS             2515..2691
                     /locus_tag="ACHURS_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240059.1"
                     /translation="MNLVDAFVTKVISGPYEEYGKWWVDVEYISWGVPGKSRLMFDSR
                     EQAQEVQEGFRFLT"
BASE COUNT          744 a          696 c          645 g          628 t
ORIGIN      
        1 ttgatattgc cgaaaagcca ggcaagaata ttcactaacc tgaacagggc ctgaaaacgc
       61 tgtcttttca ggcctctgca ggcaaaaact gatgacaaca aaatttctgc gtttgtaccc
      121 caccgatgaa aaagacaaaa actcacaccg ggcttctcat cattaaagat aaaacccgac
      181 gtgtcagcct ctacgaaacg ccaacggcat ggtgtatcag ggggcaggag tgctacagca
      241 aaagcactgg acgccgctgt ggcagccacg acagtctgag cagactgcgc ctggacagca
      301 ttaagcctgt tgaatgagtg gtggcaccat gaaagataac aacacaaccg ccaccccaga
      361 aacctggaaa atgcgttttc tggccaacgg gacatccggc acatggtttt acactgacga
      421 cattgcagtc actgacgcgt ggcttcgcgc gcacccggat gatgtggagg ttatccctct
      481 ctgccggata cagccccgcc agcattccca tgaacaacat attgaagaac tccgcgcagc
      541 actggcccgg atgctccata tgcatcatat gatgctggaa accacacatt accgggaaaa
      601 tatcaccgat gcccgttgta tccgtgaaat gcatgaagcg ccattacagg cgctcaatat
      661 tctgtccaga tacccgcata aaaaggacga ggtgtgacgc atgtggctac tgattttctg
      721 gctttcatcg ccctttaatg tctcttccgg tgatgcccgt cagcccacac tggcggtgca
      781 cagccaggcc tttcataccg aacgggcctg ccgcgatgca ttgcgcgcca ttaaaaagga
      841 gaatgttggc gaattttctc tgcggggcgt ttgtgtttct gacggaaata cgaaggagga
      901 ggctgagagc tcatgacgga tattactgac agacatacac ttcgccgtat gtcctggtcg
      961 gaactcatca cggcaggaca ggaagcagaa tttcagcatg attacgagcg tgccagaata
     1021 gtgtggtcat tcgccttaca tgttgcaaca accacgatta acaaaaatct ggccatagct
     1081 catattcgac gctgcgatac tttgttgcat aaatcaaagg ctgtacccgg aaacaacccg
     1141 tgtggttccg gtcagcgagt ctgagacctc aacatcccgg gtgatgattg tcattccgga
     1201 ctgacgcagc cgctgcacat ctgcggcaat acgttgaata ccaagccaga acagaccatc
     1261 cagcgcggtg acaattaacc cgctttccag cgccagtttc agccgtgctg tcggggcttt
     1321 aacctgcccg gctatatcag tgaaacaacg cgtcgtcatg ccatagctgt ccgcccgccg
     1381 tattcgcgtc gcaagactgt aactgaagct atccggcacg ccggataagt cattctgcag
     1441 actgtataca catccccgct gccgctcatt gaatacaact tttttctggc tgagctgcca
     1501 ttcccgccgc acgctgtcaa tcatctgcgg tgtccgggtt aacagcagac gaaacggcag
     1561 ttcaaaactg gtcacataat caacattaag taaggcaatc cgcagccgct ccctggctcc
     1621 tgctccggta tcacggcatt cacgcatgac atcttcccac tggcgcatca gtcgcggtga
     1681 ctcattcctg gccctgaact gatatttctc aatctgataa tcaatatgtt cctgcatacc
     1741 gtcaccggat atctgttgcc gtatcatcac ggatatcagc gtactggaat tcatgccctg
     1801 cgtgaagtat cacactgcca aaaaaaacac acctgaacgt tattatttcg caatattaat
     1861 tgcacctgtt ttcataaata aaaaggagac gatgaaatga taaaatatat cttttcactc
     1921 accattattt tttccgcttc tctctgtgct gcacagattc aggggcacgt catccgcgtt
     1981 cttgatggtg atacgctgga aatattacag gacaaaaagc ctgtcagggt ccgtctggct
     2041 aatattgatg caccggaaaa gaaacagccc tttggacgct ggtcagccac tcagctgaag
     2101 gacctggtgg ccggaaagcc cgtaaccgtg caatacgcgc agacagacag gtatggccgt
     2161 attctcggcc gggtctatac cgctgacggc acagaagcaa atcgctggca ggtccgtaac
     2221 ggcgctgcct gggtgtaccc ggactacaat aatgactata ctctcccgcc gctccagcgt
     2281 gatgcccgcg ccctgaagcg gggattatgg gcagataaaa atccggtacc accatggacc
     2341 tggagaaaac agaataagaa ataattattt gctgatatga agaaaaaaac catttcaaag
     2401 aaaataaatg gcaaaaatac agcatggaaa aaaattgcca tttttaccgc cgcgttattt
     2461 ttaatatttg ctgtctgctg gtcgtaataa gcagaataac agaaaggtgt aaaaatgaac
     2521 ctggttgatg catttgtcac taaagtgata tccggaccgt atgaagaata cggaaaatgg
     2581 tgggtggatg tagaatatat ttcctgggga gttccgggta aaagccggct catgtttgac
     2641 tcacgggaac aggcacagga agtgcaggaa ggttttcgat ttttaacctg agcatatttt
     2701 gttaaagggc aga
//
LOCUS       DAZPDU010000318         2696 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZPDU010000318 DAZPDU010000000
VERSION     DAZPDU010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.322"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            6..335
                     /locus_tag="ACHURS_004506"
     CDS             6..335
                     /locus_tag="ACHURS_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000855123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240060.1"
                     /translation="MKTLTFNNGTVSVGDVFVSSWGYEQTNVNFYQVISVHGKKTVTV
                     QEIRASVHRTHSMSGYKTPLLNDFCGEPLKRRVRDYYSTPAIEIEEFETAYKGSPEEK
                     HEFTSYY"
     gene            915..1238
                     /locus_tag="ACHURS_004507"
     CDS             915..1238
                     /locus_tag="ACHURS_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020314677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240061.1"
                     /translation="MENHAKFVATEILNQLGGNRFIAMTGAKNFACFDENGECGLCFR
                     LPSNFAMKGINLVKIKLTFSDTYLVTFSRVRGATVKEISKFDNIYCDQLECLFNEQTG
                     LATRL"
     gene            1279..1488
                     /locus_tag="ACHURS_004508"
     CDS             1279..1488
                     /locus_tag="ACHURS_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020314678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240062.1"
                     /translation="MKVLIGNINIDNYHMLSALAGIAGFDRSIQFTCEISASIEIMED
                     DFVNKAGILKMLDEFIENDFSIKLV"
     gene            1586..1810
                     /locus_tag="ACHURS_004509"
     CDS             1586..1810
                     /locus_tag="ACHURS_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240063.1"
                     /translation="MKQSTFPVIVSTTGHVFSVVRVTLCTICLKHEKTGEAYVVIFTD
                     CHNIRDYKKGVVPVLGELYQEDVDLITGKS"
     gene            1862..2122
                     /locus_tag="ACHURS_004510"
     CDS             1862..2122
                     /locus_tag="ACHURS_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240064.1"
                     /translation="MKIRKGDRQYYLNKEGDTFHLVKRVKTFSKSATLGKTKATVKTV
                     ADLVFHEKAFDTIDFASDGLRENDKEIVSMMIQEMSEGKNAK"
     gene            2112..2363
                     /locus_tag="ACHURS_004511"
     CDS             2112..2363
                     /locus_tag="ACHURS_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001132407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1240065.1"
                     /translation="MPNENTPENIKRLRQKIGLTQKECAEIFSMSPRTWRRKEEPVGT
                     ASGTALTPVEFKFLLLLAGEHPDYVLCKRNKKNSDSGSN"
BASE COUNT          798 a          527 c          623 g          748 t
ORIGIN      
        1 taaaaatgaa aacattaact tttaataacg gcactgtttc tgttggcgat gtgtttgtat
       61 cttcctgggg atatgagcaa acgaacgtta atttctacca ggttatttct gttcatggca
      121 aaaaaaccgt aactgttcag gagattcgcg cttcagttca tcggactcat tcaatgagcg
      181 gatataagac cccattactt aatgatttct gtggtgagcc attaaaacgg cgagtgcgtg
      241 attattacag tacgccagca attgaaattg aggaattcga aacagcatat aaaggatcac
      301 cggaagaaaa acacgagttc acatcatact actaagaaaa tagttgcagg gggtatcccc
      361 tgcaaccatg ttaaattgtt aatagtgaat acaggggctt aatcgcctct tttttatttg
      421 gtgcgcagta aaactccgtc cttcaaggcg tggagaatgc caggcgaatg cagcaatgta
      481 atgttgcgcc cgtagctcgc tgacgctcac acgcgcagta cagcgccccg ccctgctgca
      541 atgcaccagg acggcgtaaa ctcgctggcg ctcgcgctaa ctgcggcatt ctgcggcgca
      601 aatcagcatg atcatggcct gtcagtccgt tgggctttgc agggcgaagg gatggccctg
      661 tgcatcctgg tatgttcgtc agcggctgcg ccgcgcttcg gggcgatgtg gtcccgctgc
      721 tccctggtat tttctttcgc cccttctatg ccctgtcggg cattacatga atctaaccat
      781 ctgtatttga tggtatttat tttaaagaaa ggaggaaaaa acacttgata ataggtcagt
      841 tctgacctat tataaacaca tcgagagggg atggtcccca atcaccaaag ccgctcagcg
      901 gcaggagctt aaaaatggaa aaccacgcca aatttgttgc tactgaaatt cttaatcagc
      961 ttggtggaaa ccgctttatt gctatgacag gcgcaaagaa ctttgcctgt tttgatgaaa
     1021 atggtgagtg tggtctttgc tttcgtttgc cttcaaattt tgcaatgaag ggaattaatt
     1081 tggttaaaat caaattaacc ttctctgata catacctggt gacattttcc cgtgtccgtg
     1141 gagctacagt aaaagaaata tcaaaatttg ataatattta ttgtgatcag cttgaatgtt
     1201 tatttaatga acaaaccgga cttgcaacca gattgtaatt tttaatatgg ggggattccc
     1261 cccatatcag gaggataaat gaaagttctt attggtaata taaatattga taattatcat
     1321 atgctgtctg cattagctgg gattgcggga tttgacagga gcattcagtt tacatgtgaa
     1381 atatcagcaa gtatcgaaat tatggaggat gatttcgtaa ataaagctgg tatattaaag
     1441 atgttagatg aatttattga aaatgatttt tcaataaagc tcgtttagaa aatacttgat
     1501 aataggtcat ttatgaccta ttattaatgc attgagaggg gatggtcccc aatcgccaaa
     1561 gccgctcagc ggcaggagat taaatatgaa acaatctact tttcctgtta tcgtctctac
     1621 aactggtcat gtgttttctg ttgtgcgcgt gacgctttgc acaatttgcc ttaaacatga
     1681 aaaaacaggc gaggcttatg tcgtcatttt tacagactgc cataatatcc gtgattataa
     1741 aaagggcgtt gttccggttc ttggggagct ttatcaggaa gatgtcgatt tgattaccgg
     1801 aaaaagttaa aagaaagggg ctatatgccc ctttactgtt aaaaggggag actatcttga
     1861 tatgaagata agaaaaggtg acagacaata ttatctgaat aaagaaggtg atacgtttca
     1921 tctggtgaag cgagtaaaaa ctttttctaa gtctgcgact ttaggaaaaa cgaaagcaac
     1981 ggtgaaaact gttgccgatc ttgtttttca tgagaaggca ttcgacacta tcgactttgc
     2041 cagcgacgga ttaagagaga atgataaaga aattgtttct atgatgattc aggaaatgag
     2101 tgagggaaaa aatgccaaat gaaaacacac cagaaaacat taaacgtctg cggcaaaaaa
     2161 ttggcctgac acagaaagaa tgtgccgaaa tattcagcat gagtccgcgt acctggcgtc
     2221 gcaaagaaga acctgtcgga actgcaagcg gtacagcact tacaccagta gagtttaaat
     2281 ttcttctgct tttagctgga gaacaccctg attatgtact ttgtaaaagg aataaaaaga
     2341 attctgattc tggaagtaat taaggaatat taagatgaaa atagaattaa cgtatgacga
     2401 aatcacggaa taagctacaa aaaccataac tgaatttatg gctggactta atagccccag
     2461 ttgcattgaa caatgagcaa ttgcgcccgt agctcgctga cgctcacacg cgcagtacag
     2521 cgccccgccc tgctgcaatg caccaggacg gcgtaaactc gctgtcgctc gcgctaactg
     2581 cggcattctg cggcgcaaat cagcatgatc atggcctgtt ggtctgttct ggctttgcag
     2641 ggcgaaggga tggccctgtg catcctggta tgttcgtcag cggctgcgcc gcgctt
//
LOCUS       DAZPDU010000319         2669 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZPDU010000319 DAZPDU010000000
VERSION     DAZPDU010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.323"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            108..617
                     /gene="eco"
                     /locus_tag="ACHURS_004512"
     CDS             108..617
                     /gene="eco"
                     /locus_tag="ACHURS_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFR1240066.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     KGMKRQVIQLTPQEDESTLKVELLTGQTLEVDCNLHRLGGKLENKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAVVR"
     gene            complement(926..2572)
                     /gene="mqo"
                     /locus_tag="ACHURS_004513"
     CDS             complement(926..2572)
                     /gene="mqo"
                     /locus_tag="ACHURS_004513"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFR1240067.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
BASE COUNT          642 a          732 c          661 g          634 t
ORIGIN      
        1 aagagcaggt aaaattgtct gaatgttctt taagttattc ataaagcaaa ttaataaatc
       61 tgatgaatat gttaaccttc agcgacatca tcggtgaaaa cctataaatg aagaaggaaa
      121 gcaaaaaaat gaagaccatt ctacctgcag tattgtttgc cgctttcgcc accacttccg
      181 cctgggcggc agaaagcgtc cagccactgg aaaaaatcgc gccttatcca caagctgaaa
      241 aagggatgaa gcgtcaggtg attcagttaa ccccgcaaga agatgaatct accctgaaag
      301 tagaactgtt aaccggtcag acgctggaag tcgattgcaa tttgcatcgt ctcggcggga
      361 agctggaaaa caaaacgctg gaaggctggg gctatgatta ttatgtcttt gataaagtta
      421 gctcgccggt ttccacgatg atggcctgcc cggatggcaa gaaagagaag aaatttgtca
      481 ccgcgtatct gggcgatgct ggaatgctgc gttacaacag caagctgccg atcgtggtgt
      541 atacgccaga caatgtagat gtgaagtacc gcgtctggaa ggcggaagag aaaattgaca
      601 acgcggtagt tcgctaaact gctgtgaagt gcgtcacccc gtaggtcgga taaggcggtc
      661 acgccgcatc cgacatctaa cgcccaagcc ggttgcctga tgcgacgctg gcgcgtctta
      721 tcaggcctac accgctgtga agtgctccac cccgtaggtt ggataaggcg gttacgccgc
      781 atccgacatc taacgcccga gccggttgcc tgatgcgacg ctgacgcgtc ttatcaggcc
      841 tacaccgctg tgaagtgctc caccccgtag gtcggataag gcggttacgc cacatccggc
      901 ataccatgcc ggatgtggca catcattaca acgcaatatc cgccacttct ttttgaacgg
      961 gttgcggttt caactgcggt ttcggcgtac tatctgctgc ctgcggcttg tcgtatttca
     1021 ggcccagcac ttcgctggtg tactgcaact cgcgttctgt tgccgctaca tcaccgttca
     1081 gcttgcgtcc ataagatgga acgatcgctt tcaacgtagc ctgccattgc gggctggaaa
     1141 cgcgatcgcc aaatactttt tccagcagat ccaacataat cggcgcagcg gttgacgccc
     1201 ctggcgatgc ccccagcagc gcggcaatgg tcccttgctg gtcactgacc acttcagtac
     1261 ccagacgcag tacaccacct ttatcggcat cacgcttgat aatctgcaca cgctgccccg
     1321 cttgccacaa acgccagtcc tcttttttcg cctgcggata gtactctttc aacgcttcaa
     1381 aacgatcctc ttcactcaac atcacctgac tcaccaagta tttcaccaga tcgaaattat
     1441 ccagcccgac gtgcatcatc ggcatcacgt tagaggtggt ggtggaactc atcagatccc
     1501 acaatgaacc gtttttgagg aatttggttg agaaggtggc aaatggccca aacagcacta
     1561 cgcgtttacc gtccagaacg cgggtatcga tatgcggaac cgacatcggt ggtgcgccaa
     1621 cggatgcttt accgtaaacc ttcgccagat ggtgattaac cacgtccggg ttttccgaaa
     1681 caaggaactg tccgcccacc gggaaaccgg cgtagtcttt cgcttccgga atccccgatt
     1741 cctgtaacag cttcagcgcc gcaccgcccg cgccgataaa gacaaatttg gcacgaatgt
     1801 tctgtgcagt gccatttttc agatcggcaa cggtaacggt ccaggtgtta tcgtcattac
     1861 gctttagggc gcggacttcg ctgctgagtt gcagcgagaa gttagatttc ttctgcaagg
     1921 aagcaattaa ctggcgggtg atctcgccgt agttcacatc ggtaccaatt tccgtacgcg
     1981 tggctgccac tttctgttgc ggatcgcgcc cttccatcac taacggtgcc cactctttga
     2041 tctgcgcgtg atcttcagag taacgcatac cgcgaaacag cgagctttgt tgcaacgcgg
     2101 cgtaacgggc gcgcaggaaa ttaacgttat cctcgcccca gacaaagctc atatgcggaa
     2161 cggtattgat aaatgaacgc ggagtacgca gcacgccgcg ctcgacctgg tgcgcccaga
     2221 actggcggga aatctgaaat gcttcgttaa tggcgactgc tttttcaata ctgatactgc
     2281 catcggcgtt ttgcggggtg tagttcagtt ccatcagtgc agaatgcccg gttccggcgt
     2341 tgttccagcc gttcgaactc tcctgcgcga caccctccag gcgctccacc atggtcatcg
     2401 accattcagg ctccagctcg cgtaaatagg tccccaacgt ggcgctcata atgccgccgc
     2461 caatcaacag tacatcagtt tcctgctcct cggacgcttt cgcttttgcc gccatcgaaa
     2521 cggcattaag ccccacggcc atcgagaaga gcatggcagt cacttttttc atcttgttaa
     2581 tgccttactt ttagtcgctt tattgcaggt gagatttgcg cggcatcaac ggtaacacta
     2641 aagtaacaac atttaaataa tgtttataa
//
LOCUS       DAZPDU010000320         2630 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZPDU010000320 DAZPDU010000000
VERSION     DAZPDU010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.324"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            448..600
                     /gene="mokC"
                     /locus_tag="ACHURS_004514"
     CDS             448..600
                     /gene="mokC"
                     /locus_tag="ACHURS_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFR1240068.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(704..1834)
                     /gene="dnaJ"
                     /locus_tag="ACHURS_004515"
     CDS             complement(704..1834)
                     /gene="dnaJ"
                     /locus_tag="ACHURS_004515"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFR1240069.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1923..>2630)
                     /locus_tag="ACHURS_004516"
     CDS             complement(1923..>2630)
                     /locus_tag="ACHURS_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HFR1240070.1"
                     /translation="MGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKR
                     AADNKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASS
                     GLNEDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKT
                     AIESALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNA
                     KDDDVVDAEFEEVKDKK"
BASE COUNT          595 a          697 c          649 g          689 t
ORIGIN      
        1 acacggaaag tcatatcagc gctaacgggt gaccctaagc cctgataatc atagcttaag
       61 gtcaggattt ctgagggatt aattaggaaa agtaataatt aatttcttgt cagtcggcgt
      121 tatttgttag gacaatattt caaaaataat ccagcttgtc gtcaataatt gcgataaata
      181 gataaattaa agttaaataa ttaaatcatt aaaaaattac gccacaaaaa tgcgaggcgt
      241 cttcaggatt agaatcgata aaacagaatg ggttaaagag agcgatataa tagcggcggg
      301 tgcttgaggc tatctgtctc aggcattagc tgaacggcag atagagaaaa gccccgagcg
      361 atattttact atcaacccga ggccccctat atgctgaaca catgtagagt gcctcttact
      421 gaccgtaagg tcaaggagaa gagagcaatg aagcagcata aggcgatgat tgtcgccctg
      481 atcgtcatct gtatcaccgc cgtagtggcg gcgctggtaa cgagaaaaga cctctgtgag
      541 gttcacatcc gaactggcca gacggaggtt gctgttttca cggcttacga atccgagtaa
      601 gagcaacggc ggggagtgat ccccgccact tttcaggtgc tcgcatatct tcaacgcacc
      661 ctaattttac ccaagcctgc ccacgggcag gcttttgggg aggttagcgg gtcaggtcgt
      721 caaaaaactt cttcacacca tcaaagaagc tctttgagcg cgggctgttg tgctcaccgg
      781 ttgggccacc gaagctttct tgcagctctt gcagcaactg tttctgcttc tcgttcaggc
      841 ctaccggtgt ttcgacgaca acgcggcaca gcaaatcacc ctgtgcgcca ccgcggacag
      901 acttgacgcc tttaccgcgc atacggaaaa gcttaccggt ctgggtttcg ccaggcactt
      961 tcagtttgac gcgaccatca agggtcggta cttcgatttc gccacccagc gccgccatag
     1021 cgaagttgat cgggacttcg caatagaggt tgttgccttc acgctcgaaa atcgggtgct
     1081 gtttaacctg aacctgaacg tacagatcgc ctgccggtgc gccatgctcg cccgcttcac
     1141 cttcgcccgc aagacggatg cggtctccag tgtccacccc tgccgggatt ttaacggaca
     1201 gcgttttgct gcgctcaaca cgaccatgac catgacattt gttgcacgga tctttgatca
     1261 gcgtaccgcg gccctgacag tgtggacagg tctgctgtac agcgaagaat ccctggcgca
     1321 tctgcacctg accagaacca tgacaggtcg gacaggtctg cggctgtgta cctggttttg
     1381 caccgctacc gtggcaaacg tcacactctt ccagagtcgg aatgcggatc tctttggtca
     1441 cgccacgtac agcttcttcg agggtgagct ccatgttata gcgtaaatca gcaccgcgcg
     1501 ccgcacgttg acgaccacgt ccgccgccaa aaatatcgcc gaaaacgtca ccaaaaatat
     1561 cgctgaagtc tgcgccgccg ccaaaaccgc cgccgcccat gccaccttgc tcaaacgcag
     1621 catgaccata ctgatcgtat gccgcacgtt tttgcgagtc ggtcagaact tcataagctt
     1681 ccttgatctc tttaaatttc gcctcggcct ctttgtcacc ctggttacgg tccgggtggt
     1741 atttcatggc caggcgtttg taggcctttt tgatttcacg ctcttccgct gttttggaaa
     1801 cgcctaaaat ctcgtaataa tcttgcttag ccatcttttt taaattgccc ctagatgaat
     1861 gcacgggcgg agaggaaatt ccccttcgcc cgtgtcagta taattacccg tttatagggc
     1921 gattattttt tgtctttgac ttcttcaaat tcagcgtcga caacatcgtc atctttcgcg
     1981 ttgtttgcag aagcatcagc accggcagtc tgctgctggg catgttgctg ctgggcgatt
     2041 tccatcagtt tctgggaaac ctgtgccagt tcctgcattt tcgcttcgat agcggctttg
     2101 tcttcacctt tcagagcagt ttccagtgca gtcagcgcag actcgatagc agttttgtcg
     2161 tcagccggca gtttgtcgcc tgcttcttca acctgcttac gggtgctgtg cagcagatgg
     2221 tcgccctggt tgcgagtctg taccagctct tcaaacttac ggtcagcttc ggcgttagct
     2281 tctgcgtcgc gtaccatttt ctggatttca tcttcgttca gaccagaaga agccttgatg
     2341 gtgatcttct gctctttacc gctgttttta tctttcgcgg aaacgtgcaa gataccgtca
     2401 gcatcgatat cgaaggtaac ttcgatctgc ggcatgccgc gcggtgccgg gttgatacca
     2461 tccaggttga actgacccag agatttgtta tcagctgcac gtttacgttc accctgcagc
     2521 acatggatgg ttaccgcaga ctggttgtct tcagcggtag agaacacctg gctgtgcttg
     2581 gtcgggatag tggtattttt cgcgatcagc gtcgtcatca caccgcccat
//
LOCUS       DAZPDU010000321         2627 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZPDU010000321 DAZPDU010000000
VERSION     DAZPDU010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.325"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            593..2494
                     /gene="ltrA"
                     /locus_tag="ACHURS_004517"
     CDS             593..2494
                     /gene="ltrA"
                     /locus_tag="ACHURS_004517"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFR1240071.1"
                     /translation="MLPDELEKRLDGIEDASKKGYPVRNLYKIACEPGLWLQAYVNIR
                     ANAGALTQGVSTDTIDGFSIERAEKLAKTLRSREYVAKPVRRVQIPKKDGKTRPLGIP
                     GGDDKLAQEVARIILERVYEPVFSEHSHGFRPKRSCETALRSIRPVWNGVKWIVDVDI
                     KGFFDNIPHKLLLNTLAEKIKDKNFLKLIEQWLKAGYVDNWKYHRSYSGTPQGGIISP
                     LLANIYLDKLDRFVEQNLIPAYTSGTKRRANPDMNRLAHKIHKLRKKVDGMATDSESE
                     KEAIKKEIERLLEEKRSIPSQVMNDPNFRRMYYVRYADDFVIGVIGSKKDAEHISRQV
                     RNFITTSLGLEVNEAKTRIRHISEGVNFLGYEIRQADAKKLLKQKMQGRHALRRSTTG
                     IVQLFVPDNIAAKFCHQKKYGCYENVKAVHRSSLQNLSEAEIVLTFNAEMRGLANYYS
                     LALDMKYKLSKLYFIWQISLFKTLANKRRSSVNKVAKSLRQNNGDLAITVQAKNGSRK
                     IEVFKLKHVNRNRTTIVDTEPRTAHITTRTTEIMRRLGARICEYCAKTGRCEVHHVRK
                     LKDLKKGRKAGYKPSLWQLMMIARRRKTMILCASCHDKLHSGKLPDLRQEKGRILLQP
                     PVSSGDSAK"
BASE COUNT          875 a          524 c          629 g          599 t
ORIGIN      
        1 ttttatggag tgttgcatga aaagcaacat ggcttagtgg tatagccatt gcatactgtg
       61 gttagcctcg cttggtaaca ggctcggaga tcctgaaaag gagaggtgaa catagcatcc
      121 acagaaaagg gtggtgaata cggaaatcgt tataagtatc accactcaat gcaagatgta
      181 tatggataaa ctgggtttgt tgaagtcgag atctatgtgg gaaacaggaa accaataacg
      241 cagaacgatt gaagcgttat tcccgtaata gcacaagaaa tccgccctac gttgattgtg
      301 accactctaa cgggccagct attgatgaaa tagttaaacg atcgaaagac atcctaagta
      361 catcgcatct ccacaccgtg aaactacggg actacctaaa ctggatttat acccagcatg
      421 gtagcggagt ctccatagta ctcaaggtcg gcctgtaatg ggctacggca gtccgcatta
      481 tacggaagca gcaaaggtat aaatcaaccg gaagggagat gaaagcctca cagtgctgcg
      541 gggaagggag acagttgtta gatacatcaa tctctaagag gagggttagc caatgctgcc
      601 tgatgaactt gagaaaagat tagatggaat tgaggatgct tcaaaaaaag ggtatccagt
      661 acgtaatcta tacaaaattg cgtgtgagcc cggactgtgg ctgcaagcct acgtgaatat
      721 acgggccaat gcaggtgcac tgactcaagg tgtatcaaca gataccattg atggcttctc
      781 tatcgaaaga gcagagaagc tggcaaaaac attgcggagt agagaatacg tagcgaaacc
      841 tgtacgcaga gttcagatcc caaaaaagga cggaaaaacc agaccgctgg ggattccagg
      901 tggagacgat aaactggcgc aagaagtagc aagaattatt ctggaaaggg tttatgaacc
      961 agtattctct gaacactctc atggattcag accaaaacgt tcgtgtgaaa ctgcgctcag
     1021 atctattcgt ccagtctgga acggagttaa atggatcgtg gatgttgata tcaaaggttt
     1081 ctttgacaat atcccccaca aactattgct gaatacactg gcagagaaaa ttaaggataa
     1141 gaatttcctg aaattaatcg aacagtggct gaaggcagga tatgtggaca attggaaata
     1201 ccatcgctca tattctggca cgccacaagg tgggataatt tctcccttac tggcaaatat
     1261 atacctcgac aaactggacc gttttgtaga acagaactta attcctgcat atacaagcgg
     1321 tacaaaacga agagcgaatc cagatatgaa caggctggcc cataagattc ataaactacg
     1381 aaagaaagta gacggtatgg caactgattc tgaatcagaa aaagaagcca taaagaaaga
     1441 aattgaacga ctgcttgaag aaaaacgttc tataccatct caggtcatga acgatccaaa
     1501 cttcaggcgt atgtactatg ttcgatatgc tgatgacttc gtaattggtg tcatcggctc
     1561 gaagaaagat gctgaacata tatcaagaca ggtcagaaac tttattacta cttcactggg
     1621 attggaagtt aatgaagcaa agaccaggat ccgccatatc tcagaaggag ttaatttttt
     1681 aggctatgaa atcagacagg cagatgcaaa gaaattgctg aagcagaaaa tgcaagggcg
     1741 gcatgctctc cggagatcca ccacaggaat tgtgcagctc ttcgttcctg acaatatagc
     1801 cgctaaattt tgtcaccaaa agaaatatgg ctgttatgag aatgtgaagg ctgtacatcg
     1861 ttcaagtcta caaaatctca gtgaagctga aattgttctg actttcaacg cggaaatgcg
     1921 cggattggcg aactactaca gtctggcgct ggacatgaaa tataaattat caaaattata
     1981 tttcatatgg caaataagcc tttttaagac cctagcgaat aaacgacgta gttcagtgaa
     2041 taaagtcgcc aaatcgctac ggcaaaataa tggagatctg gctattaccg ttcaggccaa
     2101 aaatggatcc cgaaagattg aggttttcaa actcaaacat gtcaatcgca accgaacaac
     2161 gattgtcgat acggaaccga gaaccgcaca tatcacaacc cgcacaacag agatcatgcg
     2221 tcgattaggg gcaaggatct gcgagtattg tgcgaagact gggcgctgcg aagtacatca
     2281 tgtcagaaag ctaaaagacc tgaagaaagg tcgtaaggcc ggatataaac ccagcctgtg
     2341 gcaactaatg atgatcgcac gccgccggaa aacgatgatt ttgtgcgcaa gttgccacga
     2401 caagttacat agcggaaaac tacccgatct gagacaggaa aagggaagaa tactcctgca
     2461 accacctgtc tcaagtgggg attctgctaa atgagtttat ccccaataag ctagcaatgg
     2521 agagccgtat gcattgaaag gtgcacgtac ggttcggagg gggggtaacg gtgatctaac
     2581 tcaagataag caggacatat tcgccattta tcttgaaaga ccacacg
//
LOCUS       DAZPDU010000322         2587 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZPDU010000322 DAZPDU010000000
VERSION     DAZPDU010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.326"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            196..1149
                     /locus_tag="ACHURS_004518"
     CDS             196..1149
                     /locus_tag="ACHURS_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1240072.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(1190..2185)
                     /gene="pgl"
                     /locus_tag="ACHURS_004519"
     CDS             complement(1190..2185)
                     /gene="pgl"
                     /locus_tag="ACHURS_004519"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFR1240073.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTCWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(2481..2576)
                     /locus_tag="ACHURS_004520"
                     /pseudo
     CDS             complement(2481..2576)
                     /locus_tag="ACHURS_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
BASE COUNT          714 a          636 c          578 g          659 t
ORIGIN      
        1 tatttttttc ataataatgc cctcaattta aaaacataac ttattgcgat atggttacat
       61 taagggcaaa gcatctctaa tgactttagc tcaacgatta ataacatttt ttcattaatt
      121 taagttggct aaatgcagtt aaaattttta acggccagcc acccaaaatc gtcgcttcca
      181 ttttccggag taataatgaa gcatgaatta tcaagtatga aggcatttgt catactggca
      241 gagtccagtt cgtttaacaa tgccgctaaa ttactcaata ttacgcaacc tgcattaacg
      301 cgaagaataa aaaagatgga ggaagattta catattcaac tatttgagcg tacaactcgc
      361 aaggttacgt taacaaaagc aggaaaaagg ttgctcccgg aggcgcggga attaataaag
      421 aaattcgatg aaacgctttt taatattcgt gatatgaatg cttatcatcg tggtatggtg
      481 acgttagcgt gtattccgac cgcagtattt tattttttac cgctggcaat cggtaaattt
      541 aatgagctgt atcccaatat taaagtgcgg attctggaac aaggcacgaa taattgcatg
      601 gaatcggtgc tgtgtaacga atcagacttt ggcataaaca tgaacaacgt cacaaattca
      661 tccattgatt ttaccccact ggtcaacgag ccgttcgtgc tggcctgtcg gcgtgaccat
      721 ccattggcca aaaagcagct tgtagaatgg caggagttag tgggttacaa aatgattggc
      781 gtacgctcct ccagcggcaa ccgactgttg atagaacaac agctggccga caagccctgg
      841 aagctggact ggttttacga agtgcgtcat ctttcgacgt cattaggact ggtagaggcg
      901 ggactgggga tttcggcgct tcctggtctg gcaatgcccc atgcgcccta ttcttcgatt
      961 attggtattc cactggtgga acccgttata cgtcggacat tggggattat tcgccgcaaa
     1021 gatgccgtgc tttctccggc agcagaacgc ttttttgcct tgctgattaa tttgtggact
     1081 gacgataaag acaatttgtg gaccaatatc gtcgaacgcc agagacatgc gttacaggag
     1141 ataggttaat acaccggcgc aggagagact gcgccgggta aatcagcggt tagtgtgcgt
     1201 taaccaccac ccacattggt ccctgcccga ccgcatagcg gcctttttca tgcagtagcc
     1261 cctgctcgcc aacaatttcg tataccgaga tgtggtgaga tttttgcccg gcggcaatca
     1321 gatacttgcc gctgtgatca acattgaagc cgcgcggctg ggtttccgtt ggctggaagc
     1381 cttctttact caacacgctg ccatcttccg aaacgctgaa aacggtaatc agactggcgg
     1441 tacggtcgca ggcgtataaa tgacgaccat ccggggtgat atgaatatca gccgcccaac
     1501 aggtgtcgga gaagttttcc ggcatcatat ccagcgtctg gacacattca atattgccgt
     1561 gtggatcttt cagttcccag acatccactg agctgtttaa ctcattgacg caatacgcat
     1621 attgttcgtt tggatggaat accatatgac gcgggccggc cccttcaacg gtagtcactt
     1681 ccgcagggtc ctgcgccacc agatgaccat catcgctgac cgtaaacagg caaatgcgat
     1741 cctgctttaa tgccggaacc cacagcgtac ggttgtccgg tgagatattg gcggaatggc
     1801 aaccgtccag cccctcgacc acatcgacga cgcccactgg caggccatct tccagacgcg
     1861 ttacgctcac gttacccgca ttgtaagaac ctacaaagac aaactgcccc tggtgatcgg
     1921 tggaaatatg cgtcggacta cccggcagcg cagactctgc ggcaaaggtc agtgcgccat
     1981 cgtccggggc aatacgatac gccaggacgc gaaactcagg gcgaacacca acatagagat
     2041 aacgtttgtc cgggctgacc accatcggct gcacctgccc cggcacatcg acaacctgtg
     2101 tcagcgtcag tgcgccttca tgattcaggt tccagacgtg aatttgctgg ctctcagggc
     2161 tggcgatata aactgtttgc ttcatgaaca cacctcaaaa atgataaatc ccttatcttt
     2221 catacacata acaacactta ctggataaaa aacagacaac aaaacacgat aaaatacaat
     2281 atcgcacatc aattaaatca ataagttagc actccatttc gaatggcata ttactaaaat
     2341 attagtcacc gacacgaatt tgacaatgcc acagatattt tcgcgactat tcagggagta
     2401 tgactctccc cgcttttgtt tacctgtatc aacgaccgta aacactatgc cataacaact
     2461 tctggccaac ttcccattcg ctcaacaatc aactcacttc cggcacaaat aaacgcatta
     2521 cgttgtagat cggtattctg gtcatttgtt gatacgcgta catagccaat aagcatggta
     2581 aatcccc
//
LOCUS       DAZPDU010000323         2583 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZPDU010000323 DAZPDU010000000
VERSION     DAZPDU010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.327"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..403
                     /locus_tag="ACHURS_004521"
     CDS             <1..403
                     /locus_tag="ACHURS_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YPO3983 family protein"
                     /protein_id="HFR1240074.1"
                     /translation="IIKECIENGINSTPSTGYQPLILQSIKTKLLSSRLNKFNDFEDS
                     FNGLGISVHDISAQKISLLSFQKYAIGWSATIHFVAQDHFGLDVTDIKNKTYSKYRFF
                     RIWFFLQRHKDFAFKPFFTNFNTIERIENYL"
     gene            400..879
                     /locus_tag="ACHURS_004522"
     CDS             400..879
                     /locus_tag="ACHURS_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001688100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HFR1240075.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIERVFNPHDFK"
     gene            complement(933..1883)
                     /gene="corA"
                     /locus_tag="ACHURS_004523"
     CDS             complement(933..1883)
                     /gene="corA"
                     /locus_tag="ACHURS_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFR1240076.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(2035..2091)
                     /gene="ysgD"
                     /locus_tag="ACHURS_004524"
     CDS             complement(2035..2091)
                     /gene="ysgD"
                     /locus_tag="ACHURS_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFR1240077.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            2253..>2583
                     /locus_tag="ACHURS_004525"
     CDS             2253..>2583
                     /locus_tag="ACHURS_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240078.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPMLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLVDMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
BASE COUNT          756 a          590 c          561 g          676 t
ORIGIN      
        1 aataatcaaa gaatgtatcg aaaatggcat taattcaact ccctcgacag gataccaacc
       61 tcttatcctg caaagcatta aaaccaaatt actttcatca agactaaata aatttaatga
      121 cttcgaggac agttttaatg gcttaggaat cagtgttcat gatatttcag ctcaaaaaat
      181 atctctttta agttttcaaa aatacgctat aggatggagt gcaacaattc attttgtagc
      241 acaggaccac ttcggtcttg atgtaactga tatcaaaaac aagacttaca gcaaatatag
      301 attttttcgc atatggttct ttttacaacg gcataaagac tttgctttca aacctttttt
      361 caccaatttt aacactatcg aaagaatcga aaattatcta tgaaaagcaa actccagaaa
      421 attatattat gtttatttct tctatgctgc atctataatt tatggacatt gcgtcctgta
      481 cagattttgt atacatactc tgatgctggc aattcagtct ttctggtcgt tgatcatctg
      541 ccatggactg acagcgataa aataaattgg tatctgaagc atcagaatga aattaaaaat
      601 caacatcccc taccagaagg tagttggcat acctggtatg tcattgatat cggtaatggc
      661 ttcactgact ataaaaaata tatcgaaggg ccgtatgaag atctctactg tttcccgaca
      721 attaagagca atgataattg catcgtgaaa aattacttaa tggttattaa tgaataccct
      781 tatcgtaaca ctcatattgg tattaacgat tttactgagt atcaattaac acaagaaaat
      841 aaaatagagc gagttttcaa tccgcatgac tttaaataag acaattttta gaaataagcc
      901 ggataaaaaa atagctttat ccggcactca tattacagcc agttcttgcg cttaaagtac
      961 agatacggtg ccaggcccgc gaggatcata aagataatcg cgcctgggta gccgaaactc
     1021 cacttcagtt ctggcataaa ctcaaagttc atcccatagc tggaagcaac gagcgtcggc
     1081 ggcaggaata ccacggatac caccgagaag attttgatga tgcggttctg ctcgatgttg
     1141 ataaaaccca tcgccgcctg catcaggaag ttcaccttct ggaacaggga ttcgttatgc
     1201 ggcagcaggg attcgatatc tcgcaggatt tcacgcgcct gctccagttg cccacccggt
     1261 aaacgcgctt tacgcaccag gaagttgagc gcgcgctggg tatccatcag acacaggcga
     1321 actttccagc cgatatcttc cagttccgcc agagtggaga gcgcctcgtc gtactcatcg
     1381 ccctgatgcc cttccataat cacccggctc aactgctcca ggtcgctata gatattttca
     1441 atttcatctg ccaactgttc gattttggtt tcgaacagat ccagcagcag ctcgtaggcg
     1501 ttaccgtcta ccatcgactg gctacgggca cgcatacgat acagacgaaa agcgggcagt
     1561 tcacgctcac gcagagtaaa cagacgacca tcacggatgg taaatgccac agtggagtta
     1621 ccggcgtgat cttccgcatc ttcaaagaag aagaaggagt gaatatgcag gccgtcgtcg
     1681 tcttcaaaga aacgtgccga tgcttcgatg tcttccagtt ccgggcgggt tgccaggctc
     1741 tggccaagtt cagattgtac gcgcagtcgc tcgtcgtcgt ccggttcgac aagatcaatc
     1801 catactgcat ttacaagggg ttgtgactct tcgacttcca gccgggtcag tcggttattt
     1861 tccagttgaa atgcgctcag catgaccggg actcccaatg cttaaaatat cggacagttc
     1921 ggtgggcaca cagaaacaaa ttgggtttca gaccattaaa cagcctgact cagcgcgacg
     1981 ggaaaaattg aggtcgctga caaccgctaa ggctatcagc aaaaagggat agccttagga
     2041 gttgatcctg gatgacagga tagtgagcca gtatctactg ggtgtgtcca aggcgaatgt
     2101 cctcttagag tgatcgtgcg cgcatgttac gccagcaaaa ttttgccgtc aacacgcaac
     2161 agaacaccac agaacaatag ttgaacttcg cgctttttgg tactcgtcaa ggtcgctaac
     2221 aggataaaaa aatccaacat ccaggaccta taatggcgca tcggctactc attggtaaag
     2281 gaatgatcac cttgaatctc aaacggattt ttctcgccct caccttactt cccttgtttg
     2341 ctgttgccgc tgatgattgc gcactctcag atccgatgct gaccgtacag gcgtataccg
     2401 ttaatcctca aacagagcgg gtgaaaatgt actggcaaaa agccaatggc gaagcgtggg
     2461 gaactttaca tgctctgctg gtggatatga atagtcaggg tcaggtgcag atggcgatga
     2521 acggcggcat ctatgatgaa agctatgcgc cgctcggttt gtacatcgaa aacggtcagc
     2581 aga
//
LOCUS       DAZPDU010000324         2562 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZPDU010000324 DAZPDU010000000
VERSION     DAZPDU010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.328"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(1..803)
                     /locus_tag="ACHURS_004526"
                     /pseudo
     CDS             complement(1..803)
                     /locus_tag="ACHURS_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
     gene            complement(856..1452)
                     /locus_tag="ACHURS_004527"
     CDS             complement(856..1452)
                     /locus_tag="ACHURS_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFR1240079.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            complement(1479..2081)
                     /gene="yadL"
                     /locus_tag="ACHURS_004528"
     CDS             complement(1479..2081)
                     /gene="yadL"
                     /locus_tag="ACHURS_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HFR1240080.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGLANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            complement(2096..>2562)
                     /locus_tag="ACHURS_004529"
     CDS             complement(2096..>2562)
                     /locus_tag="ACHURS_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1240081.1"
                     /translation="ATTCNITLTGNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADF
                     KLVASGCSSGISWIDTTLTGNASSSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATF
                     LKPNSAEKIRWSTDEMQPDKGLEMTVALRETDAGQGVPGNFRALATFNFIYQ"
BASE COUNT          712 a          552 c          513 g          785 t
ORIGIN      
        1 aagcatagcg gtaaaacaag tgggattagt caacttgaca ttgagcagat cgaaatcgat
       61 tttgataaaa taactatgct catttatacc ttggcctgcg ccataggctt taaatgaata
      121 cagataattt gatgagctgg ataatttgat ctgttgagtt actgttggat caaagtttgt
      181 atctgtatag aattcaattg taagggtctg ataaatacca cctacagccc agtatttacg
      241 ggtgctattc gcattattgc aactccaata taacacgtct tcgcttggat tataccacga
      301 aaaatattgt tctgcagagt caccaatgta tatcccaggt gaactaacgt tagttacggt
      361 accgtaagct gaccacatgt ttgaaattaa taatgtgtaa tacaaaccag gaactgatgt
      421 attaaataat ttatgggagc catacatttt acctgagtca accattgcat tttcaatacg
      481 cattgcgcta ccacttgtgc tatcacttct gttgcaataa agaaaaccac caccagaagc
      541 ggtatctaac tgactccact gtaattttac acctgtaggt ccaagctgcg tcggtttgta
      601 gaaaactaaa ttatttgtac ttgttgcata tctgaatatg acagtagcat cgccattcgg
      661 ccccgtttct ctccattttt gatctgacgg accaacgccg taactattcc caacaatata
      721 tcctacgtta tcatcagtgc cagcactcgc ggtataacaa ctcaagcaca gaattaaaaa
      781 aaatgtctga agaatttctt cattatcact ccaccatcta ctgctgtaac agtcgcagtt
      841 atttgttttg atctattatt cgtagtacac atcaactaat atatgactca ttacttggcc
      901 agatatgaca tccagcgcgg gatcgatttt ttgcattctg gcataaaatt tccatgaaga
      961 attatgcagg gcagaagtca ttactggata cacggaacga gaatttcctg ctgaactaat
     1021 aacattgaat atattggtcg ggtcctgaat ggaaaagaga acgacaccga catttttagc
     1081 cccggttgcg acttcattgg caaatatttg ccttgaacct gcagccatga caccgctaag
     1141 ggctctaaaa tcaatatgta actgattaag cacatcgcca gttccctgta atgcacagtc
     1201 tgatactgtg acagtgaact cttttccacc tggttgatag ctttcaggag taacattatc
     1261 tgaaaaatat cccaccccaa cgacgccaag atcaatatta ccctggttac tgattccgct
     1321 tttgcaagtg ctgtttttta cattggcaac aaggtcaata tcctgcccag cgatggctgg
     1381 ataggtaatg ggaaactgta gtgacagaaa cagaattatt ctcttcatca gcttacgttg
     1441 agtgggatgc atatatagtt cctggatctg tcagtctgtt agttataggt tactgtaaat
     1501 gtggcaggtg cagaaaattt acccgcagtg acattgctta cggttttatt tttttcgacg
     1561 accagtcgag cactcatcgg ataataaaca accgcgttgg ctgcattgga gatagtgacc
     1621 tcttgagatg ctggtgtcac acaactaaat tgtgttgcgc tccctgttgc cggagtttcg
     1681 gtgctccaga cttcaacggc gacggcactt gctttatctg cggctgtgga tccatttgcg
     1741 agccccgcac cgtcattagc agtaccttcg catgtggctc gcttggttaa ttttatttgt
     1801 gcttttttat tggggatacc cgcacagtct ttgaatttga gtttgaatgt tttaactttg
     1861 gtcttggcat tgatttcaga aatatataca tcgccaaagt caacaacaga aatgtcctga
     1921 tcagagtcat ttactaatgt agcggtacaa gtacctattt ctacggtagt aatcacggtc
     1981 agcccaatgc tgtctgttgc cgcataagag ggtacgctga acaatgaggc agccaaaacg
     2041 acactgctgg ataatccata acgtaaattt ttaaatgtca tcttaattat ccttcttatt
     2101 gatagatgaa attaaacgtt gccagtgcac gaaaattccc cggtacgcct tgccctgcat
     2161 ctgtttcacg tagcgcaacg gtcatttcaa gacccttatc gggctgcatc tcgtctgtgc
     2221 tccagcgtat cttttccgca ctgttaggtt taaggaaagt ggcatcatca gtagtccgtt
     2281 ttttgaaacc cataccgata ttacttgtcg tcgaagatga atcaccagac tgcggtataa
     2341 taagcttagg tgagcttgat gatgcatttc cggtcagagt ggtatcaatc caactgatgc
     2401 cactgctgca cccactagca accagtttaa aatcagcctg agattctgtc gttttattcg
     2461 cgatcttatc cagacccatc ttagggattc tcaatgtgta gttattattg ccatcattcg
     2521 tgacgttatt accagtaagt gtgatgttac aggttgtcgc tt
//
LOCUS       DAZPDU010000325         2556 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZPDU010000325 DAZPDU010000000
VERSION     DAZPDU010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.329"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(58..261)
                     /gene="tatE"
                     /locus_tag="ACHURS_004530"
     CDS             complement(58..261)
                     /gene="tatE"
                     /locus_tag="ACHURS_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFR1240082.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(390..1178)
                     /locus_tag="ACHURS_004531"
     CDS             complement(390..1178)
                     /locus_tag="ACHURS_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFR1240083.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSWIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            1271..1654
                     /gene="crcB"
                     /locus_tag="ACHURS_004532"
     CDS             1271..1654
                     /gene="crcB"
                     /locus_tag="ACHURS_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFR1240084.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(1708..1917)
                     /gene="cspE"
                     /locus_tag="ACHURS_004533"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(1708..1917)
                     /gene="cspE"
                     /locus_tag="ACHURS_004533"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFR1240085.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(2092..>2556)
                     /gene="pagP"
                     /locus_tag="ACHURS_004534"
     CDS             complement(2092..>2556)
                     /gene="pagP"
                     /locus_tag="ACHURS_004534"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFR1240086.1"
                     /translation="TFRENIAQTWQQPEHYDLYIPAITWHARFAYDKEKTDRYNERPW
                     GGGFGQSRWDEKGNWHGLYAMAFKDSWNKWEPIAGYGWESTWRPLADENFHLGLGFTA
                     GVTARDNWNYIPLPVLLPLASVGYGPVTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT          653 a          659 c          580 g          664 t
ORIGIN      
        1 tttgaagcgg tttttttgta tcgaacaaga tattgaggga gcgtcctgct cgccacgtca
       61 ctctttatga gagagctttt cagcctgaag atcaacgtct gcgccttttt tcgccgcagc
      121 atcgtcatca ttcatcgcct tcttgaaccc tttaatggcc gctccaaggt ctccgcccag
      181 cgtacgtaac ttcttagtcc caaacagcag aacgaccagc gccgcaacta ccagcagttt
      241 ggtaatacta atctccccca tagatacctt cttgacatat aaccggcaac agaacgcgcc
      301 ttaaaaacca gagaatatta acggtcattc gacgcgcgca cacaatagca atctgtaaca
      361 aggtgaatca agcgccgagt aaaaaaacat cataataatt gcggcggcgc aaagcgacgg
      421 ttgtttaaga cgggcagttg cgcgcgcacc tgacgcacac gttcgggcgt cacttccgcc
      481 ataatgagtg caggcatttc tgacgctgcc gcaatggtga cgccaaaggg atctataatc
      541 cagctttgcc cgatattttt gttcccgcac tcccccgccg ccaccatata acaggtggta
      601 tccagcgcac gagcggcaag caacgttgac cagtgatgct ctttgagcgg cccgcgaacc
      661 caggcggcag gaagtaccag gatttcagct ccctgtaatg cctgtgccag cgccagctct
      721 ggaaagcgta agtcataaca ggtcatcaga ccgaccttca tcccctccac ctcgagtaac
      781 ggagcgattt cattaccagc atcaacacgg cgtgattcct gaatggcaaa tgcatcatag
      841 agatgcaatt tggcataacg ggcgacgatg tttcctgcct gtagcgccac cagcatattc
      901 catgcgcgcc ccggcgttga aggaacatga atcgtcagaa ttgtcgtcat catgttacgt
      961 ttactttctc gccgtaaacg tccgaggaat tcgccttcca gcagctgtgc tgatttaacc
     1021 gatagatctg catcatgatc atcgcgcgcc agcaatgctt ccggcaggac gaacagcgat
     1081 acgtcgtttt ccgccgcctg cgccatcaac gaggcacaaa tctcagcgtt cttttcccac
     1141 acagatgtaa cagcaaactg tcctgcggca accagcattg ccacctcctg tcagagtcgt
     1201 aaaattcgcc aacgacgatt attcggcgtt acacttatca ctcatacaaa tcaaatagca
     1261 ggattttgca gtgttacaac ttcttttagc agtttttatt ggcggtggta cgggaagcgt
     1321 ggcgagatgg ctgttaagta tgcgatttaa cccgctgcat caggcgattc cgttggggac
     1381 gctggcagca aacctgattg gggcattcat cataggaatg ggattcgcct ggttcagcag
     1441 gatgacgaac attgatccag tgtggaaagt attaatcacc accggatttt gtggcggtct
     1501 aacaaccttc tcaacatttt cggcagaagt ggtgtttttg ttacaagagg gccgctttgg
     1561 ctgggcgtta ctgaacgttt tcgtcaacct tctggggtct tttgccatga ccgcactggc
     1621 tttctggctg ttttcggcct caaccgcaca ctaaagacca aaaaaaaccc gctgattaag
     1681 cgggttttga attcttgctg acgtatctta cagagcgatt acgtttgcag cagaagggcc
     1741 tttggcaccg ttagtgattt cgaactctac gcgctgacct tcagcaagag ttttaaaacc
     1801 attagtctgg attgcagaga agtgtacgaa cacgtctttg ctgccgtctt ccggagtaat
     1861 gaaaccgaat cctttggact cattaaacca cttaacgtta cctttaatct tagacatcaa
     1921 aattaccttt acatgaaaaa tagacacaaa tgctgtgtcg gttaccagta caccaattgt
     1981 ggtacgcttt gtccagtgca actttactaa aaagtgataa aagtcgcgtt atttttgatg
     2041 ttacttattt tagttattga ttttaaagaa gttactaaaa cttcatttgt ctcaaaactg
     2101 aaagcgcatc caggcaaagt acacattgcc attgttgtag gtacccggaa tgtaggtcat
     2161 ctgaaaagtc actgggccat aacccacgga ggccaatggc agtagaaccg ggagagggat
     2221 gtaattccag ttatcgcgtg ccgttacgcc agcggtgaat cccagaccta aatgaaaatt
     2281 ttcatccgcc agcggtcgcc aggtactttc ccatccgtat ccggcaatcg gttcccattt
     2341 gttccacgag tccttaaatg ccatggcata caggccatgc cagtttcctt tttcatccca
     2401 tcgcgactga ccaaaaccgc caccccacgg tcgctcgtta tagcgatcgg ttttttcttt
     2461 gtcgtaagcg aaacgtgcat gccaggtgat ggcaggaata tataaatcat aatgttcagg
     2521 ctgttgccag gtttgtgcaa tattttctct aaacgt
//
LOCUS       DAZPDU010000326         2548 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZPDU010000326 DAZPDU010000000
VERSION     DAZPDU010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.330"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            1..82
                     /locus_tag="ACHURS_004535"
                     /pseudo
     CDS             1..82
                     /locus_tag="ACHURS_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415662.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            829..1674
                     /locus_tag="ACHURS_004536"
     CDS             829..1674
                     /locus_tag="ACHURS_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR1240087.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGIATPKSFTAHNRAQAVFLC
                     VMHSHIQIMVGRAGQSQDWPGSVMTGCSNPVRLTTHEIATSGGELIKFIAEAAIMATT
                     LSHPCVTVENGRAVTTSVAVAEFFHKRHDNVLRAIANIECSDKFTALNFEASEYTDSI
                     GRKLPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAELRQNNTPPADKMIPG
                     DGRTLVVHFDKFGNVEFTETVPDGALVCTLETFRFYLEKQGWTLVNRGAIKNMTVEQL
                     LKIHC"
     gene            1685..1873
                     /gene="dicB"
                     /locus_tag="ACHURS_004537"
                     /gene_synonym="ftsT"
     CDS             1685..1873
                     /gene="dicB"
                     /locus_tag="ACHURS_004537"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFR1240088.1"
                     /translation="METLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKHERELLNKI
                     CIVSMLARLRLMPKGCAQ"
     gene            1861..2061
                     /locus_tag="ACHURS_004538"
     CDS             1861..2061
                     /locus_tag="ACHURS_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFR1240089.1"
                     /translation="MCTMNTVFALVLTVFLVSGEPVDMVTGVYSSMKECMTAAAKQKI
                     PGDCYPVDKVIHHDNNEIPAGL"
     gene            2146..2421
                     /locus_tag="ACHURS_004539"
     CDS             2146..2421
                     /locus_tag="ACHURS_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240090.1"
                     /translation="MITSYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLNDGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
BASE COUNT          745 a          557 c          599 g          647 t
ORIGIN      
        1 agatgcaaaa cgcctccagc aactggagcc aaatgcaggc actggggcac gcatctggct
       61 ggctaaagaa gcgctggaat ctggcgatta tgatagcgaa gaagccttct acaaagcaga
      121 aggccgtaca ggatattcac cgggtcttgg cggggtataa ataccatgcg cattgactga
      181 attcgcaaac aaaaacagac gcgcgatacc tggatagtcg gtctgcatag tcagatatct
      241 ggctgcgaat ttgataaata tcaccgccct tttcggaaac ataagttccg tccgggagtt
      301 gattatacga accatctcca tgggggatcg ttctcctgaa tcctagtgag agcatatatt
      361 tatgaagccc ttcgtaatcc tctggctcag cattatatag ttctactctg gcgagatacg
      421 ttggcatatt catttcctta ctggttgtgt gagaacttca gtaaagatac caccaaagcc
      481 cggaggtggt gaaataaaac cgggcacaac acgaaggcgc atttccggta tccataaaga
      541 gtcggtcttg tctgttaaat ttaaatggtg ggagtgcgcc tccggttgta aataacgaca
      601 ttgctgtgtg aagtaccagt tggcggcatc ggtttaattg ctggctgatg tccgcccttt
      661 ttaaagtgaa ttttgtgatg cggtgaatgc ggctcagcgc acgcggaaca gttaaaacag
      721 taaggaaaat cccttattcc ggcgttaatt gttaactggt taacgtcacc tggaggcacc
      781 aggcaccgca tcaacaaagt tcacttcggt gatgaaagga aagagaaaat gttgaatgta
      841 gctattgaaa accagaacgg gtggaattat agtgcacctg cacctcataa aacgggtgcc
      901 gggattgcta ccccgaagtc attcacggcg cataaccgcg ctcaggcggt ttttttatgc
      961 gtaatgcaca gccacattca aattatggtg gggcgtgcgg ggcagtcgca agactggccg
     1021 ggttccgtga tgaccggttg tagcaaccct gtacgtctca ccacccatga gattgctacc
     1081 tccggtggtg agttaatcaa attcatcgcg gaggctgcca tcatggctac taccctttct
     1141 cacccttgtg taaccgtaga aaacggtcgc gctgtcacta cgtctgttgc ggttgcagag
     1201 ttcttccaca aacgacacga caatgtgtta cgtgccatcg caaatattga atgctcagat
     1261 aaatttactg ccctcaattt tgaagcgagc gaatacaccg actcaatcgg gcgcaaactc
     1321 ccaatgtacc aaatcaccaa aaacggcttc gttttcctgg ttatgggctt cactggcaaa
     1381 aaagccgctg cattcaaaga agcctacatt gctgagttcg accgcatgga ggccgaactg
     1441 cgccagaata acaccccacc tgctgacaag atgattccgg gtgatggtcg cactctggtt
     1501 gttcacttcg acaaattcgg caatgtcgaa ttcaccgaaa ccgttcctga tggcgcactg
     1561 gtctgtaccc tggagacttt ccggttttat ctggaaaaac agggatggac tcttgttaac
     1621 cggggcgcaa ttaaaaatat gactgtggaa caattactca aaattcattg ttgaggacgc
     1681 aataatggag acgttattac caaacgttaa tacgtctgaa ggttgttttg aaattggtgt
     1741 cactatcagt aaccctgtat ttactgaaga tgccattaac aagagaaaac acgaacggga
     1801 gctattaaat aaaatatgca ttgtttcaat gctggctcgt ttacgtctga tgccaaaagg
     1861 atgtgcacaa tgaatacagt atttgcactc gttctgacgg tttttcttgt ttccggtgaa
     1921 ccggttgaca tggttactgg cgtatacagc tcaatgaaag aatgtatgac tgccgcagcg
     1981 aaacagaaaa ttcccggtga ctgttatccg gtcgataaag ttattcacca tgataataac
     2041 gaaatcccgg caggtcttta aaacagttcc gtaataaata tccggtttca ttcttatatg
     2101 ccagcaatgg caggggttcg ctcaacctta attaaggaga aaaacatgat taccagttat
     2161 gaagccactg ttgtaactac tgatgacatt gttcacgagg ttaatctgga aggaaagcgt
     2221 attggctacg tgattaagac agaaaataaa gaaaccccat tcactgtggt tgatatcgac
     2281 ggtccatcag gcaacgttaa aacacttaac gatggtgtaa aaaaaatgtg tctggtgcac
     2341 ataggaaaga atctgcccgc agaaaaaaaa gccgaatttc tggcaactct gattgcaatg
     2401 aaattaaaag gtgaaatctg aaaaaaagaa agcctgcaca ctgtgcaggc ctgagtgaag
     2461 aacctgggac atttattcat cactcgcagt aattttaatc tgagttgagg ttaaaaaaca
     2521 atgagcaccg ataaacaagt ttacccac
//
LOCUS       DAZPDU010000327         2520 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZPDU010000327 DAZPDU010000000
VERSION     DAZPDU010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.331"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(89..173)
                     /locus_tag="ACHURS_004540"
     tRNA            complement(89..173)
                     /locus_tag="ACHURS_004540"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(137..139),aa:Leu,seq:tag)
     gene            complement(182..258)
                     /locus_tag="ACHURS_004541"
     tRNA            complement(182..258)
                     /locus_tag="ACHURS_004541"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(222..224),aa:Met,seq:cat)
     gene            complement(638..2302)
                     /gene="asnB"
                     /locus_tag="ACHURS_004542"
     CDS             complement(638..2302)
                     /gene="asnB"
                     /locus_tag="ACHURS_004542"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFR1240091.1"
                     /translation="MCSIFGVFDIKTDAVEQRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            complement(2256..>2520)
                     /locus_tag="ACHURS_004543"
     CDS             complement(2256..>2520)
                     /locus_tag="ACHURS_004543"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240092.1"
                     /translation="APVKYNHSIKPAKNYRFINKYILTPAPFSPIDNHHYVFYFFTAK
                     SLAHSAALRHFFKQANTTSNKYQVNGEGYVFNFWRIRYQNRRS"
BASE COUNT          608 a          638 c          622 g          652 t
ORIGIN      
        1 acctggattc gaaccaggga atgccggtat caaaaaccgg tgccttaccg cttggcgata
       61 ccccatccgt acaacgcttt ctggtgaatg gtgcgggagg cgagacttga actcgcacac
      121 cttgcggcgc cagaacctaa atctggtgcg tctaccaatt tcgccactcc cgcaaaaaag
      181 atggtggcta cgacgggatt cgaacctgtg accccatcat tatgagtgat gtgctctaac
      241 caactgagct acgtagccat ctttttttcg cgatacctta tcggcgttgc ggggcgcatt
      301 atgcgtatag agccttgcag cgtcaacctc tttttcaagg aaaattgctc gaaagtgact
      361 gtttggttag gttgcgaaca gcgtggcgct atattcgtca attattgttt actttgtgtt
      421 ttttccccac cctacagcca ttcttttgtc atacaggatg aaattcggaa ttcaacaatg
      481 gtggtgggaa attaatctat gaaatactgg cctacagtga tgagttgtca aacagtgatg
      541 tggcaaaccc ggaacatttc cttactgcat atcagaatca acaagcacct caataactga
      601 aacagccccg gatttcaccg gggctgtttc gcatttctta cttatacgcc gactggtgaa
      661 cgccaaccgc acgaccagac ggatcgtcca ttttcttgaa cgcctcatcc cactcaatcg
      721 ctttagcgga agaacacgcg acggacgggc cgcccggcac gcactcagcg gcgctcggaa
      781 gcgggaacag ttcttcaaag atctcccggt acagatacgc ttcttttgag gttggcgtgt
      841 tgtacgggaa gcggaagcgg gcagtttcca gttgctgatc agaaacctgc tgcgccgcca
      901 cttctttcag ggtgtcgatc caactgtaac cgacgccatc ggagaactgc tctttctgcc
      961 gccaggccac gcttgcgggc aggtatgact caaaacattc acgcaggatg tgtttttcca
     1021 ttttgccgtt accgcacatt ttatcctgcg ggttaatgcg catcgccaca tcaaggaatt
     1081 ttttgtcgag gaacggaacg cgtgcttcca cgccccaggc tgacatcgct ttgttggcgc
     1141 gcgcgcagtc atacatatgc agggccagca gtttacgcac cgtctcttca tgcagttctt
     1201 tagcgttggg cgctttatgg aagtaaagat aaccgccaaa cacttcatca gaaccttcac
     1261 cggacagcac cattttaatg cccatcgcct tgatcttacg cgacattaaa tacatcggtg
     1321 ttgaagcgcg aatagtggtc acatcataag tttcgatgtg gtaaatcacg tcgcggatgg
     1381 catccagacc ttcctgtaca gtgaagtgaa tttcgtgatg caccgtgccc agatggtttg
     1441 ccacttcctg ggctgcttta agatccggtg aacccggcag acctacagca aaggagtgta
     1501 actggggcca ccaggcttca gagcgttcct gatcttccac gcgacgggct gcgtatttct
     1561 tggtgatagc ggaaataatt gaggaatcca gaccaccaga aagcagcaca ccgtaaggca
     1621 catcagacat cagatggctt ttaacggaat cttccagagc ctgacgcagc tcgtttttgt
     1681 cggtcacgtt atctttcact gcatcaaagt cgaaccagtc gcgatgataa taagaacgga
     1741 tttcgccgtc ctggctccac aaatagctcc ccgccgggaa ctctttaatc gtgcggcaaa
     1801 ctggcaccag cgatttcatt tctgaggcca catacagctg accgtgttcg tcatacccca
     1861 tatacagtgg gatgatcccc agatggtcgc gaccaatcag gtaggcatct ttttcgctgt
     1921 cgtacagggc aaaggcaaac atgccctgca agtcgtcgag aaattccggc cctttttcct
     1981 gatacagcgc gaggatcact tcacagtcag accctgtctg gaactgataa cgatcgccat
     2041 attcagcgcg caatgcctgg tggttgtaga tttcaccgtt taccgccagt acgtgggttt
     2101 tttgttggtt gtagagaggt tgcgcccccg cgttaacgtc aacaattgac agacgttcgt
     2161 gagcgagaat ggcgttatcg ctggcataaa taccggacca gtccgggcca cgatgacgca
     2221 tcaggcgtga cagctcgagt gctttcttac gctgctcaac tgcgtctgtt ttgatatcga
     2281 atacgccaaa aattgaacac ataaccttct ccgttaacct ggtatttgtt gcttgttgtg
     2341 tttgcttgtt taaaaaaatg ccgcaaagca gcactgtgcg caagcgattt ggcggtgaaa
     2401 aaataaaaaa cgtaatggtg attgtcgatt ggtgaaaaag gggctggtgt gaggatgtat
     2461 ttattgatga atcgataatt tttagcgggt tttattgaat ggttatattt tacgggggcc
//
LOCUS       DAZPDU010000328         2518 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZPDU010000328 DAZPDU010000000
VERSION     DAZPDU010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.332"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            366..509
                     /locus_tag="ACHURS_004544"
     CDS             366..509
                     /locus_tag="ACHURS_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFR1240093.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            522..731
                     /gene="cspE"
                     /locus_tag="ACHURS_004545"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             522..731
                     /gene="cspE"
                     /locus_tag="ACHURS_004545"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFR1240094.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            897..1706
                     /gene="rlmA"
                     /locus_tag="ACHURS_004546"
     CDS             897..1706
                     /gene="rlmA"
                     /locus_tag="ACHURS_004546"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFR1240095.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLSVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFTWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1703..2269)
                     /gene="mntP"
                     /locus_tag="ACHURS_004547"
     CDS             complement(1703..2269)
                     /gene="mntP"
                     /locus_tag="ACHURS_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFR1240096.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT          666 a          594 c          611 g          647 t
ORIGIN      
        1 ttctctttag ggtaattgat agccgttaac cgactgtttt atgagaaaaa gtgatataac
       61 tttttattca ttgcatagca aaaaatgtga tattgcacgc actatgtaat aacttctccc
      121 actggcctgg aacaactgaa cttattgaac tatgttagaa aatacgccag tttaagtatc
      181 tgcctgaact ggcaaggtta agcacaatga tatatcggcg cgtattccgt tgcataagtg
      241 tgcaaaaaaa gtggaagacg tatcgagatt tgtgcgtctg atcgagacat gtttaaaaat
      301 ggcttgccat aattaacgtt gtatgtgata acagatttcg ggttaaacga ggtacagttc
      361 tgtttatgtg tggcattttc agtaaagaag tcctgagtaa acacgttgac gttgaatacc
      421 gcttctctgc cgagccttat attggtgcct catgcagtaa tgtgtcagtt ttatctatgt
      481 tatgcctgcg ggcgaagaaa acaatctaag gaatttttca aatggcaaag attaaaggtc
      541 aggttaagtg gttcaacgag tctaaaggtt ttggcttcat tactccggct gatggcagca
      601 aagacgtgtt cgtacacttc tccgctatcc agggtaatgg cttcaaaact ctggctgaag
      661 gtcagaacgt tgagttcgaa attcaggacg gccagaaagg tccggcagct gttaacgtaa
      721 cagctatctg atcgaatcca ctgatctgaa gtgtgaatac gcttcaatct cgctataaag
      781 cctcgtcgaa tgcgaggctt tttactatgc tttatcttcg ctcctggcgt tcggatattt
      841 gcccgccgcg tgattcgcgt tacacttgcg gcctttagta tcctgccgga gttgtcatgt
      901 ctttttcctg tccactttgc catcagcctc tttcgcgtga aaaaaacagc tatatctgtc
      961 cccagcgaca tcagtttgat atggcgaaag aagggtatgt caatctgctg tccgttcagc
     1021 ataaacggtc tcgtgatccg ggcgacagcg cggaaatgat gcaagcacgc cgcgcattct
     1081 tagatgccgg acattatcag ccgctgcgtg atgcaattgt cgcccaactg agggaacggc
     1141 ttgatgataa ggccacggcg gtgctggata ttggctgtgg tgaagggtat tacacacacg
     1201 catttgccga tgcgttgccc gaaatcacca cgtttggtct ggatgtttcg aaggtagcga
     1261 taaaagcggc ggcgaaacgc tatccgcagg tcactttttg tgtcgcttcc agccaccgtt
     1321 tgccgttttc cgataccagt atggacgcca taatacgtat ttacgcgccg tgtaaagcag
     1381 aagaattagc acgagtagtg aagcccggcg gctgggtcat tactgccacg ccgggaccgc
     1441 gacatttgat ggagctgaag gggctgattt acaatgaagt acatcttcat gcacctcatg
     1501 cagaacaact ggaaggtttt acattacagc agagtgcgga gttgtgttat ccgatgcgtc
     1561 ttcgcggtga tgaagccgtc gcattattgc agatgacgcc gtttacctgg cgtgcgaagc
     1621 cagaagtctg gcaaacactg gcagcaaaag aagtgttcga ctgccagacg gactttaata
     1681 ttcacctctg gcagcgttct tattaaccgt ggaagtgcgt ccagaggatc tggacgccga
     1741 tgccgatcag taccagcccg ccgagaattt ccgctttttt cccaataatt gagccgataa
     1801 agcgaccaac catcatccct aatgttgaca taatcaaggt tgcacaacca atggccaatg
     1861 cggtcgcgat aatgttgacc tgcaggaaag caagaccaac acccacagcc atggcatcca
     1921 ggctggtggc aatcgcggtg gttaccagta gccagaaacc gtgtcgacgg cgcggctctt
     1981 catcttcatc atctgcgcca cgaaaaccct caataatcat tcgcccgccg aggaatatca
     2041 gcagcacaaa cgcaatccag tggttccatt caaggacaaa ccggctggct aacatgccca
     2101 ttccccagcc gatcagcggc gtcagggttt cgacggcacc aaaaataagg ccggttcgca
     2161 atgcttcaga aaatttcggt ttatggaggg tggcaccttt accgattgat gcagcaaatg
     2221 catccatcga cataccaaac gcaagaagaa cagtagcagt gatattcatg acaatgtcct
     2281 gaccggggta tccataaaac acatcatcgc ccccagcaaa catgcataca tgaacaacaa
     2341 gccgtgatgg ttttcgcgtt catgcaggca tagcgattga tgtgcatatg gtctcgcctg
     2401 actgaaagta ttcagtccgt acgtgccacg ttttgcaacg agtatgttga cacgtacatt
     2461 tccggaatct ggaaatcggc tactccccaa atgagggcgc aaccttaaca tattttga
//
LOCUS       DAZPDU010000329         2500 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZPDU010000329 DAZPDU010000000
VERSION     DAZPDU010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.333"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..275
                     /locus_tag="ACHURS_004548"
     CDS             <1..275
                     /locus_tag="ACHURS_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240097.1"
                     /translation="ITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLR
                     LQDQQEETSVTLFQGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
     gene            complement(665..799)
                     /locus_tag="ACHURS_004549"
                     /pseudo
     CDS             complement(665..799)
                     /locus_tag="ACHURS_004549"
                     /inference="COORDINATES: protein motif:HMM:NF024729.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(812..1633)
                     /locus_tag="ACHURS_004550"
     CDS             complement(812..1633)
                     /locus_tag="ACHURS_004550"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR1240098.1"
                     /translation="MFSHPVYLFLREFSLQDSRGGSLSKKAESLSSGKITIGTKEKVI
                     IINELRQCHPLSQLLVIADLPRSTFYYHVKRLNAPDPYQLVKQVILRIYHQHKGRYGY
                     RRIRLACRNEGILLNGKTIRKLMKELGISSLIRRKKYRAYRGEQGRTCNNLLKRQFYA
                     DRPNQKWVTDVTEFKVDGRKLYLSPIMDLYNGEIVSYNLTERPLASMVKSMLLDAVEQ
                     LNKDDKPLLHSDQGWQYQMPRWQRWLSDNGITQSMSRRGNCLDNAAMESFFSTLK"
     gene            1622..2500
                     /locus_tag="ACHURS_004551"
                     /pseudo
     CDS             1622..2500
                     /locus_tag="ACHURS_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612078.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    1902..2018
                     /locus_tag="ACHURS_004551"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          654 a          565 c          588 g          693 t
ORIGIN      
        1 ccataacaga ggccaccctg tacgaaaacg ggctgtatac gcgccacatc agactcagtc
       61 gcccagtatc tgacagcaaa taccccttgc cacccggatc tcagctcatt aaaaatatga
      121 ccttacgact gcgcctgcag gatcagcagg aagaaacatc cgtgacatta tttcagggaa
      181 aaatgagtac cgacggaaat aactattaca tcatcaaagg taaagtaagc tcagtacttc
      241 tgttattaaa aatgctgcag attaatcacg cctgatctct gtcccggtat tttagcccga
      301 ggtatattct gaaatatcat tcatcatgac cggcgtttat acaaacgccg gtcattcgct
      361 aacagcttca ccgttccttc ccccatccag gttccgcatg agcgtgttgt aatgttcata
      421 agcgtattat caggattgtc tggaaaattc ccgatacttt ggactattac tcctgaacaa
      481 ttcgaaattc agcagattaa aggtaagcgt taacggaggt aagcgttaac ggaggtaagc
      541 gttaacggag gtaagcgtta acggaggtaa gcgtaaaccg actgccgtat gtagccatta
      601 agccagtatt ggtaacgtag gtgcccacct tttcaaatga tctgcgcccc aaatcttgga
      661 catcctatga aaaatgttta agtcgatatt gaaccggact catgccatcg agtttctctt
      721 taatgcgatg gtgattgtag tattcaatgt aatcaattat atcttgcttt agatgatgta
      781 acttattgta tttatttagg tagtaacatt cttattttaa agtactaaaa aagctttcca
      841 ttgctgcatt atctaagcag ttaccacgtc gggacatgct ttgtgttatg ccattatcac
      901 tcaaccaacg ttgccaacga ggcatctgat attgccaccc ctgatcacta tgcaacaagg
      961 gcttatcgtc tttattgagt tgttctaccg catctaatag catgctcttt accattgatg
     1021 ctaatgggcg ctcagtaagg ttatatgaga ctatttctcc attatacagg tccatgattg
     1081 gtgataaata gagcttcctg ccatcgacct taaactctgt gacatctgtt acccactttt
     1141 gatttgggcg gtcagcataa aattgtctct tcagtaaatt attacaagtt cgcccctgtt
     1201 ctccacgata ggcacggtac ttcttgcgcc tgataagtga gctgataccc agttccttca
     1261 ttaactttct gatggtttta ccattaagta aaatgccctc gttacggcag gcaagtctga
     1321 tgcgtcgata accataacgc cctttatgct gatggtatat tctcaatatt acttgtttaa
     1381 caagttgata tggatctggc gcattaaggc gtttcacgtg atagtaaaat gtactacgtg
     1441 gcaaatccgc gataaccagt aattgtgata atggatgaca ttgccttaac tcattaataa
     1501 tgattacttt ttcttttgtt ccaatcgtaa tttttcctga gctaaggctt tcagcttttt
     1561 tagataatga accgccccgg gaatcctgga gactaaactc cctgagaaag aggtaaacag
     1621 gatgactaaa aatactcgtt ttcccccgaa gtccgtcaac gggcagtccg tatggttctg
     1681 gaaagtcagg gcgaatatga ctcacaatgg gcggtaattt gttccattac tccaaagatt
     1741 ggctgtacac cggagactct gcgtgtctgg gttcgccagc atgagcggga taccaggggc
     1801 ggtgatggag ggctcaccac cgctgaacgt cagcgtctga aagagctgga gcgtgagaat
     1861 cgtgaactgc gctgcagtaa cgatatcctt cgccaggctt ccgcttattt tgcgaaggcg
     1921 gagttcgacc gcctctggaa aaaataatgc cactgctgga taagctgcgt gggcagtacg
     1981 gggtcggacc ggtatgcagt gaactgcata ttgccccgtc aacgtattac cactgttagc
     2041 aacaggacat catcctgata aacgcagtgc ccgtgcgcag cacgatgact ggctgaagag
     2101 agagatacag cgcgtatacg atgaaaatca tcaggtgtac ggtgtgcgta aagtctggcg
     2161 ccagttgtta cgggaaggaa tcagggtggc cagatgtaca gtggcgcgcc tcatggcggt
     2221 tatgggactt gtcggtgttc tccggggtaa aaaggtccgc actaccgtca gccggaaaac
     2281 cgttgccgca ggtgaccgcg taaaccgtca gttcgtggca gaacgtcctg accagctgtg
     2341 ggtggctgat tttacttacg tcagcacatg gcagggcttc gtctatgtgg cgttcatcat
     2401 tgatgtgttt gccggataca tcgtgtggtg acgagtctca tcgtctatgg aaacgacatt
     2461 cgtgctggat gcactggagc aggcgttgtg ggcccgtcgt
//
LOCUS       DAZPDU010000330         2483 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZPDU010000330 DAZPDU010000000
VERSION     DAZPDU010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.334"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..507
                     /locus_tag="ACHURS_004552"
     CDS             <1..507
                     /locus_tag="ACHURS_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1240099.1"
                     /translation="KIHNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNF
                     NNKILKHIKSHLIMLSAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDT
                     IDGLKEFDKYKTRILDSGINCKKYPWLYFPLAKSSPGMIVMACNKNYNQHEVADCLTL
                     SYREVNRD"
     gene            500..982
                     /gene="yqeJ"
                     /locus_tag="ACHURS_004553"
     CDS             500..982
                     /gene="yqeJ"
                     /locus_tag="ACHURS_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeJ"
                     /protein_id="HFR1240100.1"
                     /translation="MIDYKKNLLFILVFISGFILFTVYSYTAEKMIYNETCTANWVIF
                     NDQGRANLTIDFMYNKKNKTGTVALSGTWQQGNRESKSIRRNIEYTWIENYDTAHLTS
                     KKVNKFEIMDQVDDDRLAQLIPDFYVFPEKSVSYNILKQGKHAFILSIGNRAIMHCAR
                     "
     gene            complement(1016..1096)
                     /locus_tag="ACHURS_004554"
     CDS             complement(1016..1096)
                     /locus_tag="ACHURS_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240101.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
     gene            complement(1132..1556)
                     /gene="yqeK"
                     /locus_tag="ACHURS_004555"
                     /pseudo
     CDS             complement(1132..1556)
                     /gene="yqeK"
                     /locus_tag="ACHURS_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417326.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeK"
     gene            1986..2476
                     /locus_tag="ACHURS_004556"
                     /pseudo
     CDS             1986..2476
                     /locus_tag="ACHURS_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          924 a          414 c          401 g          744 t
ORIGIN      
        1 aagatccata atgacattaa catacaaaat catgtaataa actcgtcaac agacgcacat
       61 acacacaatg ccccacctgc cataaaagtt aatgcgggat acaaagagag cattggtggc
      121 gcaaagaatt tcaataacaa aatcctcaaa catataaaaa gtcatctaat tatgttgagc
      181 gcatttgtca taggcgcata ttctgcatat tggctatgga ataataatca accgaagcca
      241 tttttcaagg attacaagac tgttgcggaa attaatggct gccattttaa tgtaacagaa
      301 gatacaattg atgggttgaa agagttcgat aaatataaaa cacggatact ggattcgggg
      361 attaactgta aaaaatatcc gtggttatac ttccctcttg caaaatcctc accggggatg
      421 attgttatgg catgcaataa aaactataac caacatgaag tggcagactg tttaaccctg
      481 tcttaccgtg aggttaatcg tgattgatta taaaaaaaac ttattattta ttttagtttt
      541 tatcagtggg tttattctct tcactgtcta ttcttacact gcagaaaaaa tgatatataa
      601 cgaaacatgc actgcaaatt gggtaatttt taatgaccag ggacgagcaa atcttaccat
      661 cgactttatg tataacaaaa aaaataaaac aggaactgtt gcgttgagtg gaacctggca
      721 acaaggtaac agagaaagta aatcaatacg gcgaaatatt gaatacacat ggattgaaaa
      781 ctatgacaca gcccatttaa catcaaagaa agttaataag tttgagatta tggatcaagt
      841 tgatgatgat agacttgcac aacttattcc tgatttttat gtctttccag aaaaaagtgt
      901 aagctataat atactaaagc aaggtaagca tgcttttatt ttgagcattg gtaacagagc
      961 aataatgcat tgtgcaaggt aaactaaaat agataacaaa aaagatggaa cctcgttaat
     1021 cataaaacaa taaaattaaa tattctcgca gtatatggca gtctaaagca tcaaagtttt
     1081 gatcaacatc tttcatttta gacatctcct tgcaatgtaa aatatcatgt atcaacaaca
     1141 tctggtgcaa atccatgagt cgaactcgac attcatctta gctcgattat tattccttcg
     1201 tacagtcgat gtaaacaata cagaaagagg attattaaga accagtttac tcaggaatca
     1261 atagaggaaa ggactaacgt ttctttaaaa gaattgattt catcatctgt taaactaaac
     1321 tcatcattga cagatcgtga gatataactg tttttaactt tactctttac gttgacttta
     1381 ttgacagaat taacattcac atatcttgaa tttaatgtcc atgatgttgt ttgcgtgaga
     1441 acattctcag cattaaggaa tttttttaac ggaatccttg gtttcttttt cgagtccgaa
     1501 tttacaatat catgcatata gaccatttca tgaatctgcg aaaactcaat gtccattgta
     1561 tacctcacat ttttaccgtg actcgatgtt actgttcaat aatcaccttc catcaatact
     1621 aaaattaata cccctaatgt gccgataaca aatatagtca ttctacgtaa cgtctccata
     1681 aggtgataac cttgatcaaa tgaatttcga atataataaa ggaaatcttt tgagatgttt
     1741 atcacgaata aagctattta tgttaccaat atcatttcga gtgcgcacac tatctatctg
     1801 ttttttcgag atataaaaaa caacgtgtaa gttatgtaaa ttgccgatgc tactgagtaa
     1861 cattatggta aaaaaagtaa aaatgagcga tttttgatag aataaagcat gagaaaaggt
     1921 gatatttgac attatcagaa gctgcgaatt cggattttgc tctaatcaaa tgagagaaat
     1981 gtaaaatgaa gccacgaaat attaataata gcctaccact gcaaccatta gttcctgatc
     2041 aggagaacaa aaataagaaa aatgaagaga aatccgttaa tccagttaaa atcacaatgg
     2101 ggtctggttt aaattatatt gaacaagaat ctcttggagg aaaatatcta acacatgatt
     2161 tgtcaataaa gatagcggat atttctgaag agataattca gcaagcaata ttatctgcta
     2221 tgagcatata taaattttcg ataacagatg atttaatgag tatggctgta aatgaactca
     2281 taaaactgac caaaatagag aataatgtag acctgaataa attcactact atatgcacag
     2341 acgttctatc ccccctcgtc accagacata ataaagaaaa aaaacaaacg acattctacc
     2401 cttctcaaaa atcccctttt taattttcat tgaaaaaatg gcaattgata ctatgaatcc
     2461 aggtcataca cattaaggca ttt
//
LOCUS       DAZPDU010000331         2356 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZPDU010000331 DAZPDU010000000
VERSION     DAZPDU010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.335"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            1..247
                     /locus_tag="ACHURS_004557"
                     /pseudo
     CDS             1..247
                     /locus_tag="ACHURS_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            349..1521
                     /gene="istA"
                     /locus_tag="ACHURS_004558"
     CDS             349..1521
                     /gene="istA"
                     /locus_tag="ACHURS_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family transposase"
                     /protein_id="HFR1240102.1"
                     /translation="MLSREDFYMIKQMRQQGAYIVDIATQIGCSERTVRRYLKYPEPP
                     ARKTRHKMVKLKPFMDYIDMRLAENVWNSEVIFAEIKAMGYTGGRSMLRYYIQPKRKM
                     RPSKRTVRFETQPGYQLQHDWGEVEVEVAGQRCKVNFAVNTLGFSRSFHVFAAPKQDA
                     EHTYESLVRAFRYFGGCVKTVLVDNQKAAVLKNNNGKVVFNSGFLLLADHYNFLPRAC
                     RPRRARTKGKVERMVKYLKENFFVRYRRFDSFTHVNQQLEQWIADVADKRELRQFKET
                     PEQRFALEQEHLQPLPDTDFDTSYFDIRHVSWDSYIEVGGNRYSVPEALCGQPVSIRI
                     SLDDELRIYSNEKLVASHRLCSASSGWQTVPEHHAPLWQQVSQVEHRPLSAYEELL"
     gene            1521..2318
                     /gene="istB"
                     /locus_tag="ACHURS_004559"
     CDS             1521..2318
                     /gene="istB"
                     /locus_tag="ACHURS_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family helper ATPase
                     IstB"
                     /protein_id="HFR1240103.1"
                     /translation="MHELEVLLSRLKMEHLSYHVESLLEQAAKKELNYREFLCMALQQ
                     EWNGRHQRGMESRLKQARLPWVKTLEQFDFTFQPGIDRKVVRELAGLAFVERSENVIL
                     LGPPGVGKTHLAIALGVKAVDAGHRVLFMPLDRLIATLMKAKQENRLERQLQQLSYAR
                     VLILDEIGYLPMNREEASLFFRLLNRRYEKASIILTSNKGFADWGEMFGDHVLATAIL
                     DRLLHHSTTLNIKGESYRLKEKRKAGVLTKNTTPISDDEMVESGQHQ"
BASE COUNT          617 a          585 c          651 g          503 t
ORIGIN      
        1 cgtgcttttt cttgatgagc tacctgaatt tgaacggcgt acactggatg ccttgcgaga
       61 gccgattgaa tccgggcaga tccatctttc acgcacacga gcaaaaataa cctatccagc
      121 ccgtttccag cttgttgcgg cgatgaatcc cagccctacc ggacattatc agggaaacca
      181 taaccgctgc acgccagaac agacattacg ttatctcaac cggctctcgg ggccctttct
      241 cgaccgctgt cagcgccagt gatataagac ggtaattcgc catttggatt gtccgctcca
      301 cccaacatgt tgtttcctta aggttctcac accagaaagg acatcaacat gctgagcaga
      361 gaggactttt acatgataaa gcaaatgcgc cagcagggcg cgtacatcgt cgatattgcg
      421 actcagattg gttgctctga acggacggtc agacgctacc tcaaataccc tgaaccgcca
      481 gccagaaaga cccgccacaa aatggttaag ctgaaaccgt ttatggatta catcgacatg
      541 cgcctggcag agaatgtctg gaatagcgag gttatcttcg cggagattaa ggcaatgggt
      601 tatacgggcg gacgttccat gctgcgttac tacatccagc ccaaacgtaa aatgcgtccg
      661 tcaaaaagaa cagttcgctt cgaaactcag cctggatacc agctccagca tgactggggc
      721 gaagttgagg tggaggttgc cgggcaacgg tgcaaagtta actttgcggt taatacgctg
      781 gggttctccc gcagcttcca tgtcttcgcc gcaccaaaac aggatgctga gcatacctac
      841 gaatcactgg ttcgcgcctt ccgctacttc ggtggttgtg tgaaaacggt gctggttgat
      901 aaccagaagg ctgcggtgct gaagaataac aacgggaaag tcgtgttcaa ctccggattc
      961 ctgttgctgg ctgaccacta taacttcctg ccacgggcat gccgtccacg cagggccaga
     1021 acaaaaggta aggttgagcg gatggtgaaa tacctcaagg agaacttctt cgtccggtac
     1081 cgcaggttcg acagcttcac tcatgtcaat caacaactgg agcaatggat agccgatgtg
     1141 gctgacaaac gggagcttcg ccagttcaaa gaaacgccgg aacagcgctt cgcgctggag
     1201 caggaacatc tgcagccgtt accggataca gacttcgata ccagttactt cgatatccgc
     1261 catgtgtcct gggacagcta tatcgaggtt ggtggtaatc gttacagcgt tcccgaagcg
     1321 ctgtgtggtc agccggtatc gatacgaata tcgctggatg acgagttgcg gatctacagt
     1381 aatgagaaac tggtggcctc acatcgcctc tgttcagcat cgtctggctg gcagacagtg
     1441 ccggagcatc acgccccgct ctggcagcag gtcagtcagg tggaacatcg accactgagt
     1501 gcctatgagg agctgttgtg atgcatgaac tggaagtcct gctgagtcgc ctgaaaatgg
     1561 agcatctgag ttatcacgtt gaaagcctgc tggaacaggc agctaaaaaa gagctgaact
     1621 accgggagtt cctgtgcatg gcgctacagc aggaatggaa cggcaggcat cagcgcggta
     1681 tggagtccag gctgaagcag gctcgtctgc cgtgggtcaa aacgctggag cagttcgact
     1741 ttaccttcca gccgggcatc gaccgtaagg ttgtccggga actggctggt ctggcgttcg
     1801 tggagcgcag cgaaaacgtg atcctgctgg gccctcctgg tgtcggaaaa actcatctgg
     1861 ccatagctct tggcgtgaaa gcggtggatg cgggacatcg ggtactgttt atgccactgg
     1921 acagactgat cgcgacactg atgaaagcga aacaggaaaa ccggctggag cgtcagctac
     1981 agcaactgag ttatgcccgg gtgttgatcc tggatgaaat aggctatctg ccgatgaaca
     2041 gagaggaagc cagtctgttc ttccggctac tgaaccgtcg atatgaaaaa gcgagcatca
     2101 tactgacgtc aaacaaaggg ttcgcagact ggggagaaat gttcggagat cacgtactgg
     2161 caacagcgat cctggatcgg ctgctacatc actcaaccac gctgaatatc aaaggagaga
     2221 gttaccggtt aaaagagaaa cgtaaagctg gagtgctgac caaaaacaca acgccaatca
     2281 gtgatgatga aatggtggaa agcggacagc atcagtaacg aaagtattta gcgggcatga
     2341 aaatggcaaa taacgg
//
LOCUS       DAZPDU010000332         2331 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZPDU010000332 DAZPDU010000000
VERSION     DAZPDU010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.336"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(25..315)
                     /locus_tag="ACHURS_004560"
     CDS             complement(25..315)
                     /locus_tag="ACHURS_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFR1240104.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(305..742)
                     /gene="ratA"
                     /locus_tag="ACHURS_004561"
     CDS             complement(305..742)
                     /gene="ratA"
                     /locus_tag="ACHURS_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFR1240105.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            913..1395
                     /gene="smpB"
                     /locus_tag="ACHURS_004562"
     CDS             913..1395
                     /gene="smpB"
                     /locus_tag="ACHURS_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFR1240106.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            1610..1972
                     /gene="ssrA"
                     /locus_tag="ACHURS_004563"
     tmRNA           1610..1972
                     /gene="ssrA"
                     /locus_tag="ACHURS_004563"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2171..>2331)
                     /locus_tag="ACHURS_004564"
     CDS             complement(2171..>2331)
                     /locus_tag="ACHURS_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFR1240107.1"
                     /translation="VKLSMPELEELAAAMVQAQVDRNDEIYRRQREMKEELSGLEDLG
                     TIRAFDVT"
BASE COUNT          643 a          621 c          516 g          551 t
ORIGIN      
        1 caatgagcac cttttttctg tctgttattt attcgctgat ttttctgctc gttgcctgcg
       61 aagctctttc ggatcggcaa tgagagggcg ataaatctcc acccgatcgc catcatgcac
      121 actatcgctt agttttgccg gacggctgta aatgccgact ttgtttttag ttaaatcgat
      181 atcggtacgc aattccagca agccactggc gcgaatagct tcttcaaccg tcgcgccctc
      241 ctgcagcgtc acgcgctgca ggtactgctt ctcaggtagc gcataagcca cctcaacggc
      301 aattttacct agcactgtaa acctctttcg cacgaaccgt aaaagcctgg accatattag
      361 acgccagctc tttaaacacg cgaccaaagg cgagttcaat caacttattg gtaaactcaa
      421 agtcgagatg aaactcgata cgacacgcct cctggctcag cggcgtaaac ttccatccac
      481 caatcaattt cttgaacggc ccatccacca gattcataag aatactttgg ttactggtca
      541 actggttgcg ggtagtaaac gttttgctga tcccagcctt agagacatct accgccgcag
      601 tcatctgccc aggagtggac tccagaatcc gacttccggt acaacccggc aaaaactgag
      661 gataagactg aacgtcattc actaactgat acatttgctc cgcgctgtag ggtaccagtg
      721 cggtccggct aatctgaggc ataactattt ccatcaacaa aaatccaaca aataatatca
      781 tttatcctgc tgtaaaaaaa acgctatccc ggcgctgggt aacatcgggt tcatgctaag
      841 atagagcctt gtcccccgca ggattgatat ggggtgtttt cgatttcaga ttaccgatga
      901 ttcacgacgc ttatgacgaa gaaaaaagca cataaacctg gttcagcgac catcgcgctt
      961 aacaagcgcg cccgtcacga atactttatc gaagaagagt tcgaagcggg acttgccctg
     1021 caaggctggg aagttaaatc cctgcgcgca ggaaaagcca atatcagcga cagctacgtc
     1081 cttctgcgtg acggagaggc atttctgttt ggcgctaaca tcacgccaat ggccgtggcc
     1141 tccacgcatg tggtgtgcga tcctacccgt acccgcaagt tacttctcaa ccagcgcgaa
     1201 ctggactcat tgtacggtcg cgtcaatcga gaaggctata ccgtagtggc gctctccctg
     1261 tactggaaaa atgcctggtg caaagtgaaa atcggcgtcg ccaaaggtaa aaaacagcac
     1321 gataaacgtt cagatatcaa agaacgcgaa tggcaggtgg ataaagcacg tatcatgaaa
     1381 aacgcccacc gttaaacctg cactccaatt attgaccagt tcctcaccgc gcctccctct
     1441 ccggcggcgc gaatgaacat cttattggct atcacatccg acacaaatgt tgccatccca
     1501 ttgcttaatc gaataaaaat caggctacat gggtgctaaa tctttaacga taacgccatt
     1561 gaggctggtc atggcgctca taaatctggt atacttacct ttacacattg gggctgattc
     1621 tggattcgac gggatttgcg aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa
     1681 aagccgcaaa aaatagtcgc aaacgacgaa aactacgctt tagcagctta ataacctgct
     1741 tagagccctc tctccctagc ctccgctctt aggacgggga tcaagagagg tcaaacccaa
     1801 aagagatcgc gtggaagccc tgcctggggt tgaagcgtta aaacttaatc aggctagttt
     1861 gttagtggcg tgtccgtccg cagctggcaa gcgaatgtaa agactgacta agcatgtagt
     1921 accgaggatg taggaatttc ggacgcgggt tcaactcccg ccagctccac cacttttgac
     1981 aggactgcaa ccggacagag actataaaaa cagtcactta cggacactga ccagacagta
     2041 agcggaccag aaaaagacaa aaatatacac gcaaaatgca cgtgcatttc aaaaaagcct
     2101 ctgattatga agtcagaggc ttttctagtt gtaaataagg gtaacaattt aacacgatgc
     2161 agcttaccaa ttacgtaaca tcaaacgctc taattgttcc caaatcctcc agaccactca
     2221 gctcctcttt catttcacgc tgacggcgat aaatctcatc gttgcgatcg acctgcgcct
     2281 gcaccattgc tgccgccagt tcttccagtt ccggcatcga cagtttcacc t
//
LOCUS       DAZPDU010000333         2285 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZPDU010000333 DAZPDU010000000
VERSION     DAZPDU010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.337"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            254..763
                     /locus_tag="ACHURS_004565"
     CDS             254..763
                     /locus_tag="ACHURS_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFR1240108.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYHDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            760..2178
                     /gene="phrB"
                     /locus_tag="ACHURS_004566"
     CDS             760..2178
                     /gene="phrB"
                     /locus_tag="ACHURS_004566"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFR1240109.1"
                     /translation="MTTHLVWFSQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGVGSVWLNEL
                     IWREFYRHLITYHPSLCKHRPIIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDLEGEFIRQWLPELRNVPGKSVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
BASE COUNT          581 a          542 c          600 g          562 t
ORIGIN      
        1 aaaagagaaa atggagtgcg attgttagtt tgaaatacaa ttaatagtga ggtaattaag
       61 ctttgagaac tgttattttc taaaaataaa aaatacaaaa aatgaatgca gctcgggaaa
      121 tagtagaata attctcactc agatgaagtt gataatattt ttaccaacaa aatacttccc
      181 cgcaaaaact ctgccccata aaattcattt accatcgtct acacttactc ctttataccg
      241 ttcaggagca gtaatgaatc tacaacgatt tgatgacagc accctaatcc gtatctttgc
      301 ccttcatgag ttacatcgac tgaaagaaca tggcttaacg cgcggggcgc ttctcgatta
      361 tcacagccgc tataaactcg tctttctggc gcattctcag ccggagtacc gcaaacttgg
      421 cccgttcgtg gctgatattc accagtggca aaatctggat gactattaca accagtaccg
      481 ccaacgcgta gttgttttgc tttctcaccc cgccaacccg cgcgatcaca ccaatgtttt
      541 gatgcacgtt cagggttatt ttcgcccgca tattgattcc acagaacgcc agcagctggc
      601 tgcgcttatc gacagttatc gccgtggcga gcaaccactt cttgcgccgc tgatgcgtat
      661 caaacactat atggcgcttt atcatgacgc ctggctttca gggcagcgtt atttcgaact
      721 ttggccgcgt gtgattaact tgcgccattc aggagtttta tgactaccca tctggtctgg
      781 tttagccaag atttacgtct gcacgataat ctcgcactgg ctgccgcctg ccgcaattcg
      841 tctgcacgcg tgctggcgtt gtatatcgct acaccacgcc agtgggcgac gcataacatg
      901 tcgccgcgtc aggctgaact tatcaatgct caactgaatg ggctacaaat agcgcttgcg
      961 gaaaaaggta ttcctttatt gttccgtgaa gtggatgact ttgtcgccag tgtcgaaata
     1021 gttaaacagg tgtgcgcgga aaacagcgtt acccacctgt tttataacta tcagtatgaa
     1081 gtgaatgagc gggcgcggga tgtggaagtt gaaagagcgc tgcgtaacgt ggtgtgtgaa
     1141 ggatttgatg acagcgtgat cctgccgcct ggcgcggtga tgaccggtaa tcacgagatg
     1201 tacaaagtct ttacgccttt taagaatgcc tggctgaaac ggctgcggga agggatgccg
     1261 gagtgcgtcg ctgcgccaaa agttcgtagt agcggatcga tagagccctc gccatccatt
     1321 acgctgaatt atcctcgtca gtctttcgat actgcgcatt ttccggtgga agaaaaagcg
     1381 gcgattgcgc aattacgcca gttttgccag aacggtgccg gagaatatga gcaacaacga
     1441 gattttccgg cagtggaagg caccagccgt ttgtcggcca gcctggcaac gggcgggtta
     1501 tcgcctcgcc agtgcttgca tcgcttgttg gctgaacagc cgcaggcgct ggacggtggg
     1561 gtcggtagtg tctggcttaa tgagctgatc tggcgcgagt tttaccgtca cctgataacg
     1621 tatcacccct cgttgtgtaa acatcgtcca attattgcct ggacggatcg tgtacagtgg
     1681 cagagcaatc ccgcacattt acaggcctgg caggaaggca aaacgggata cccgattgtt
     1741 gatgccgcta tgcgtcagct taacagcact ggctggatgc ataacaggct acggatgatt
     1801 acagccagtt ttctggtgaa agatttattg atcgactggc gcgaaggcga gcgatatttc
     1861 atgtcgcagc tgattgatgg tgatttggca gccaataacg gtggctggca gtgggccgct
     1921 tcaaccggaa ccgatgcagc gccgtatttt cgtattttca acccgacaac ccagggcgag
     1981 aaatttgatc tcgaaggtga gtttatccgc cagtggctac cggaactacg caatgtaccg
     2041 gggaaatcgg tgcatgagcc gtggaagtgg gcgcagaaag caggtgtgaa gctggattat
     2101 ccgcaaccga tagtcgagca caaagaggcg agagtacaaa cgttggcagc gtatgaggcg
     2161 gcgcggaagg ggaaataaca gacgcgtcag gcaatcgagc ccagatgccg gatgcggcgt
     2221 gaacgcctta tccagcctac aaatttgtgc aaattcaata aattgcagga aatacgtagg
     2281 cctga
//
LOCUS       DAZPDU010000334         2270 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZPDU010000334 DAZPDU010000000
VERSION     DAZPDU010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.338"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(132..1136)
                     /gene="argF"
                     /locus_tag="ACHURS_004567"
     CDS             complement(132..1136)
                     /gene="argF"
                     /locus_tag="ACHURS_004567"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFR1240110.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKTFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            1298..1714
                     /gene="rraB"
                     /locus_tag="ACHURS_004568"
     CDS             1298..1714
                     /gene="rraB"
                     /locus_tag="ACHURS_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFR1240111.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            complement(1760..2263)
                     /locus_tag="ACHURS_004569"
     CDS             complement(1760..2263)
                     /locus_tag="ACHURS_004569"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1240112.1"
                     /translation="MNNIAPQSPVMRRLTLLDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLTGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
BASE COUNT          580 a          559 c          551 g          580 t
ORIGIN      
        1 aaattaaatc ctccggaaga tttacgtata gcaataaaaa aattggggtg gaataattga
       61 actatcactg cataaaataa tgtgacgccg ggatgttttt gtatttcccg gcatctttat
      121 agcgatatca attatttact gagcgtcgcg accatcaccg ctttgatggt atgcatgcgg
      181 ttttccgcct gatcaaatac aatgctggcg gcagattcga agacctcatc agtcacttcc
      241 attccgccat gtaggccaaa ctcttccgcc atttttttgc caagcgtcgt ttggtcgtca
      301 tgaaacgcgg gcaggcagtg gaggaatttg acctccgggt taccggtcaa ctgcatcatc
      361 ttgctgttca cttgataatc acgcagcaat gcaatgcgtt cagcccattt ctcttttgct
      421 tcacccatcg acacccatac atcggtatag ataaagtcag caccttcaac tcctttagct
      481 acatcttccg tcagcgtaat attcccgcca ttttgttgtg ccagggcgcg gcattccgta
      541 accagcgcag cttccggcca gcatgcctgt ggcgcgacca gacgcaaatc cagaccggta
      601 agcgctgcag cttcgagcat cgaattgccc atgttgttac gtgcgtcacc tgcatagact
      661 agcgtcattt cgttgaacgt tttgccgggc aaatgctcct gcatggtgag gagatccgcc
      721 agcagctgcg tggggtgaaa ctcattggtc aggccgttcc ataccggcac gccagcgtat
      781 tccgccagtg tttcgacaat ctcctgacca tagccgcgat actgaatacc gtcatacatg
      841 cgaccaagca cgcgggcagt gtctttaatc gactctttat gaccaatttg gctgccgctt
      901 gggccgagat aagtaacacg agccccctgg tcatatgcgg caacttcgaa agagcatcgg
      961 gtacgggtcg agtctttttc gaagatgagc gcgatgtttt taccagtgag tttggcttct
     1021 tctttaccgc ttttcttatc ggctttcagc ttcgcggcta actgcagcag gctgttgagt
     1081 tcagctggcg tgaaatcgag taatttcagg aaatgcttat gataaaaccc ggacataggt
     1141 ccctcctgtg gctaacgcct taatgaatta aaattcaatt tatatggatg attattcatt
     1201 tgcaagtcta aagcataaat ctttatcaca aaggtggagg caatgtcagt ggtgtgtgac
     1261 aataagagta tcggcaggac attaagagga atgagccatg gcaaacccgg aacaactgga
     1321 agaacagcgt gaagaaacac gtttgattat tgaagaatta ctggaagatg gcagcgatcc
     1381 ggacgcgttg tacaccatcg aacaccatct ttccgcagac gatctggaaa ctctggaaaa
     1441 agcggcagtt gaagcgttta aactcggtta cgaagtgacc gatccagaag agctggaagt
     1501 tgaagatggt gatatcgtga tttgctgcga catcctcagc gagtgcgcgt tgaatgccga
     1561 tctgatcgat gcccaggttg aacaactgat gacgctggcg gagaaatttg acgttgagta
     1621 cgacggttgg ggcacttact ttgaagatcc taacggcgaa gatggcgacg atgaagattt
     1681 tgtcgatgaa gacgatgacg gggttcgcca ctaattaatt gattaaaatg cccgatagca
     1741 catactagcg ggcatttttt tagagttcgc gtagcatccg cacttcacaa tcgacatggc
     1801 ccgtgcagcc aagcgcatag tcgatatgct caaagcccaa atgctcataa agcgcaatgg
     1861 cttcctttaa aaaagcggtc gtttccagat agcagcgttt gaagcccatc tctcgtgcct
     1921 gctccatcgc cattaaggcc agttttttcg ctagcccttt accgcgaata gccggaagaa
     1981 aatacatctt ttgcagttcg caaatgtccg actcactccc ggttaatggc gcaatcccac
     2041 cgccgccgac cacttcaccc tcgtactcaa ccacccaata tgcatggcca ggctgactat
     2101 acacttgata cagctcgtca agattcggat cggcgacggt gtagccttta tcagcggtaa
     2161 gaccgtattc ggcggatacc tgacgaatga cgcgggcgat agcaggatta tccagcagcg
     2221 tcaggcgacg cattacaggt gattgcggcg caatgttatt catagtttaa
//
LOCUS       DAZPDU010000335         2229 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZPDU010000335 DAZPDU010000000
VERSION     DAZPDU010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.339"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..301
                     /locus_tag="ACHURS_004570"
     CDS             <1..301
                     /locus_tag="ACHURS_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240113.1"
                     /translation="RVELYYRFNHEEEYYPSHRFMLVVDMKTVMVFSQRAESLGLYSN
                     NRLLINIIAEHIHSDIYLTKYQQQYPDANALINTLHESGNDMVQDEKHLSDSKNR"
     gene            390..>2229
                     /locus_tag="ACHURS_004571"
     CDS             390..>2229
                     /locus_tag="ACHURS_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HFR1240114.1"
                     /translation="MIFFNDIKPTGWLKNKMEWYMNGHIGELDKLVPHLITEHKIYGR
                     DRITPRTVSAEDGVIKKQNNPDDNMMQYYWWDSETQSNWKDGYCRSAYLLDNKEWQEK
                     ASDLCLELIDTQDDDGYIGIYDSSLKFNCKAENGEFWAQATALRFVLGCYESTRNESL
                     LTSLTQAAQCIMAGYPKETSHPFVVEQGFAGHSHGLTITDVFYRLYEITNNIEYLSYC
                     LWLYEDYSAGCVSEQDLQYKNVIDPGYLLKGHGVHTYEHIRALAIAASQRPQYQTALD
                     IFLSQLKYYLTPSGAPIGDEWITGRTANATYTGYEYCSVHELFSTYVMLIKLTTNIGL
                     ADDAEWLFYNASFGMQHPILHAIMYCKTDNCYEANERKHPDSNQFNTRYKYSPTHEEA
                     AVCCVPNTARTIPAFVENMFQENNNGFTALFYGPCEFYGTFNNVAIKITQLTEYPHDL
                     SVKCLIEPESSVRFALSFRYPGWAKMMIINGVTFTKNDTQNSLIILDRTWQHHDVIDI
                     KFIADIQFNTDLCGDTYISRGPVLYAIELESDILIKKNLLNGKYFDSGYIPCSRADES
                     IQFSYADMESFSYSTSPEGKQYIEGCFYLNKTKIVRKLIPFGQTILR"
BASE COUNT          704 a          398 c          424 g          703 t
ORIGIN      
        1 acgtgtagaa ctgtattacc gctttaacca tgaagaagaa tattaccctt ctcaccgttt
       61 tatgcttgtt gttgatatga agacagtaat ggttttttca caacgagctg aatcactggg
      121 gttatactcg aataatcgct tgcttatcaa tataattgcc gaacatatcc atagtgatat
      181 atatctaacc aagtatcagc aacaatatcc agatgccaat gcattaataa atactttgca
      241 tgaatctggc aatgatatgg tgcaggatga aaaacatttg agtgatagca aaaaccgtta
      301 atattttcct ttcattgtga tcgtctgtag gcgatcacaa tctatttgtc ttttataacg
      361 tctaaacttt tttatgcaaa ggtgtattta tgattttttt taacgatata aagcccactg
      421 gttggttaaa gaataagatg gaatggtata tgaacggtca tataggagaa ctggataaat
      481 tagttcccca tcttattacg gagcataaaa tatatggaag agatcgtata actccacgca
      541 ctgtatctgc tgaagatggc gttattaaaa aacagaataa tcccgatgat aacatgatgc
      601 aatattattg gtgggatagt gaaactcaat cgaattggaa ggatggatat tgccgcagtg
      661 cctacttgtt agataataaa gaatggcagg aaaaagcgag cgatttatgt ttggaactta
      721 tcgatacgca agatgatgat ggctacattg gaatttatga ttcatcatta aaatttaact
      781 gtaaagcaga aaacggagag ttctgggcac aagctactgc cttgcgtttt gtgcttggat
      841 gttatgagtc cacccgtaat gagagtttat taacttctct tacccaagcc gcacaatgca
      901 ttatggctgg ctaccctaaa gaaacgtcac atcctttcgt tgtggaacag ggttttgctg
      961 gacatagcca cggattaaca ataacagatg ttttctaccg cctttatgaa ataaccaata
     1021 acattgaata tctctcatat tgcttgtggc tgtatgaaga ttattccgca ggatgcgtgt
     1081 cggagcaaga tctgcaatat aaaaatgtca tagaccctgg ttaccttctt aaaggtcatg
     1141 gcgtacatac gtatgagcat attagggcat tagccatcgc agcgagccaa cggccgcagt
     1201 atcaaactgc attagatatt tttttatccc agttgaaata ttatcttaca ccatctggcg
     1261 ctcccattgg tgatgagtgg attacaggac gcacagctaa tgccacttat acaggctatg
     1321 aatattgttc tgttcatgaa ctatttagca cttatgttat gttaataaaa ctgaccacta
     1381 atattggtct ggccgatgat gctgagtggc tattctataa tgctagtttt ggcatgcagc
     1441 atcccatact tcacgcgata atgtactgta agactgataa ttgttatgaa gcaaatgaaa
     1501 ggaaacatcc tgacagtaat cagtttaata ccagatataa atactcccct actcatgagg
     1561 aggctgctgt ctgctgcgta ccgaatacag caagaaccat accggctttt gtcgaaaata
     1621 tgtttcaaga aaataacaat ggttttactg cattatttta tggcccgtgt gagttttatg
     1681 gcacatttaa taatgttgca attaaaatta cacaattaac tgaataccct catgatttaa
     1741 gcgtaaaatg tctcattgag cctgagtcat ctgttcgttt tgctctcagt ttccgttacc
     1801 ccggctgggc aaaaatgatg attattaatg gtgttacttt tactaaaaat gatactcaaa
     1861 actcacttat tattttagat agaacatggc aacatcatga tgtgattgac attaaattta
     1921 tagctgatat acagtttaat actgacttat gtggtgacac ttatatttcc agaggtcccg
     1981 ttttatatgc aattgagctt gagagcgata ttttaatcaa aaaaaacctg ctgaacggga
     2041 aatattttga ctcaggatac ataccctgta gtagagctga tgaatctatc cagttctctt
     2101 atgctgatat ggaatctttt tcatattcaa cctcaccaga gggcaaacaa tacattgaag
     2161 gctgttttta tttaaacaag actaaaatag tcagaaaact tataccattt ggtcaaacca
     2221 ttcttcgca
//
LOCUS       DAZPDU010000336         2168 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZPDU010000336 DAZPDU010000000
VERSION     DAZPDU010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.340"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(468..1019)
                     /locus_tag="ACHURS_004572"
     CDS             complement(468..1019)
                     /locus_tag="ACHURS_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR1240115.1"
                     /translation="MAILGYCRVSTDDQSITNQQMQIEEAGYNIAKWFTDEAVSGSVK
                     ASLRNGFSRLLAYAREGDTVVVVAVDRLGRDTIDVLSTVKALQAKGVTVISLREGFDL
                     SSAMGEAMLGIMSTLAQLERSLIAERRKAGIERAKAEGVHMGRPVKASSEAVQTLISQ
                     GKTRLQIQEELGISRATYYRLAK"
     gene            1818..2114
                     /locus_tag="ACHURS_004573"
     CDS             1818..2114
                     /locus_tag="ACHURS_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFR1240116.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
BASE COUNT          628 a          467 c          422 g          651 t
ORIGIN      
        1 aggcttactt agtggaaaga gaggggatat gtgaccatag gggaacccct cctgttaaaa
       61 gactacttag aagtctgagg ttttagtaaa atctgtgaat atggcttctc agacgctctg
      121 taacgcattc taagcgattt taccccttta ttttaaggtg gttagtacat ccctataggt
      181 tacccctaaa aatggggtta aaactcgcta cagagctttc tatgagatta gcttctaatc
      241 gaatcaacta tgaggtttgg taggattgtg caaaatctgt acagtaactt ttcagaatct
      301 gaaaattttt ataaaaaatg actagccctc tttgaatctc atcttccccg aatcttcaaa
      361 aatatctgac agatgtctgt agctgctaag tacccgtcac accagctatg tgaccccctt
      421 ggcaaaaaag ccaaatatca gctacatcca tgtaactatt cagtaactta tttagcaagt
      481 ctgtaataag ttgctctaga gattcctaac tcttcctgaa tctgcaacct cgtcttacct
      541 tgtgaaataa gcgtctgtac agcctctgaa gaagctttta caggcctccc catatgtacc
      601 ccttcagctt tagcacgttc aataccagcc tttctacgct ctgctatcaa gcttctttca
      661 agctgtgcca acgttgacat aatgcctaac attgcttcac ccatagcgct tgataagtca
      721 aacccttctc taaggctgat aactgtcaca cctttagcct gtagcgcttt aaccgttgat
      781 aacacatcaa ttgtgtcacg tccaagacgg tctacagcta ccacaactac ggtgtcacct
      841 tctcgtgcat aagctaatag tctgctgaaa ccgtttctta aagaagcttt gacagaacca
      901 ctgacagctt catcagtgaa ccatttagca atgttgtacc ctgcctcttc aatttgcatt
      961 tgctggttag tgatggattg gtcatctgta ctcaccctgc aatatccaag tatcgccata
     1021 atgttcccct tagaagtctc aaaagttagt ctcataagtt tagctgtctc ataatccaaa
     1081 aacaaccttt tgagactact tgagggtgca ttctaccgtt acgttgaaag tgtctcaaaa
     1141 ggaagtcctt ttaagactgg ttatgaagga gctatgaggt tgattagggg ttggtacaag
     1201 cccaactaac cgaatcaact atgaagattt taggagggta actgtaacca tcactttcta
     1261 actttcacgt aggaaattgt taagtggttt gatagttaca ccatgtgacc aagcatctat
     1321 atatcgttat atcgccctgt atatagcact atatgcagtg tcgagagagg aacgactaac
     1381 acgatacagt ttaccctgaa aagagacttc acagattatt tatatatttc aataagttag
     1441 ggtaaaaagg gactataccg agactgtata gaatctgtgt agaattgcat agatacttgg
     1501 aaatctacta ttaaattgca tagggactca cctgatattg tcctgttttt aacaggtctc
     1561 ctagaaaacc gaatagactt cattcaaggg tcattgaagt cataatcatt tcagtagaaa
     1621 aaccaagtct cgattttaaa tgcaaatagc aatcatattc aataaggcaa gacgatcagg
     1681 atcagtgaaa atgtcatttt tggcgtaatt gaccttactt tatgcatggt cttaacaaag
     1741 acatcatctt tccgttgcag atcttcgccc tttcaaataa cgtactttac aactttcccg
     1801 aacaaggagt tgtgcccgtg ttgtatgtta tttacgctca agacaaagct gatagcctcg
     1861 aaaaacgcct ttccgttcgt ccggcacatt tagcacgttt acagttactg catgatgaag
     1921 gtcgtttgtt gacagctggt ccaatgccag cggtagacag taacgatcca ggggctgcgg
     1981 gatttactgg ctcaaccgtt attgctgaat ttgaatcgct ggaagcagct caggcctggg
     2041 ccgatgctga cccgtacgta gcggcaggtg tctatgaaca cgtttcagtg aaaccattta
     2101 agaaagtgtt ctgaatagag attcaatatt taagggcacg gtttttgcaa ccgtgttctt
     2161 gtttggcc
//
LOCUS       DAZPDU010000337         2103 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZPDU010000337 DAZPDU010000000
VERSION     DAZPDU010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.341"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..547
                     /locus_tag="ACHURS_004574"
     CDS             <1..547
                     /locus_tag="ACHURS_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240117.1"
                     /translation="QIQNIGVNQIQTVGVNQVETVGSNQIIKVGSNQVEKVGIIRALT
                     VGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNNVTFSVGKTMKENTG
                     QTAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLEGESDVNGDAPVINWNCGATQ
                     PVPDAPVPKDLPPGMPDMRQF"
     gene            623..>2103
                     /locus_tag="ACHURS_004575"
     CDS             623..>2103
                     /locus_tag="ACHURS_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFR1240118.1"
                     /translation="MGGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGI
                     TYANPVNPVNPVLGAKVLPGETDLALPCPLPFILFRAYSSYRTRTPAPVGVFGPGWKA
                     PFDIRLQIRDEGLILNDSGGRSIHFEPLFPGEISYSRSESLWLARGGVAAQHSSQPLS
                     ALWQVLPEDVRLSPHVYLATNSLQGPWWILSWPEPPAYRVLTVVVDGFGRSLTFHRAA
                     EGDVAGAVTGVTDGAGRRFHMALSTQAQRAEASRKQRASSLSSPASPRSVSSSQVFPD
                     TLPAGTEYGADNGIRLEAVWLTHDPAYPDEQPTAPLARYTYTAGGELRAVYDRSGTQV
                     RGFTYDAEHAGRMVAHHYAGRPESRYRYDDTGRVTEQVNPEGLDYRFEYGESRVIITD
                     SLNRREVLYTEGEGGLKRVVKKEHADGSITRSEYDEAGRLKAQTDAAGRRTEYRLHMA
                     SGKLTSVVLPDGRTVRYGYNNQLQLTSVTYPDGLRSSRKYDRQG"
BASE COUNT          443 a          536 c          733 g          391 t
ORIGIN      
        1 ccagatccag aacattggcg ttaatcagat acagacggtt ggtgtcaacc aggtggaaac
       61 ggtgggcagt aaccagatta tcaaagtggg atcaaaccag gttgaaaagg tggggatcat
      121 tcgtgcgctg acggtgggtg tggcttacca gacgacggta ggcggcatta tgaatacctc
      181 ggtggcgttg ttgcagtcct cacaggtagg gctgcataaa tcactgatgg tgggaatggg
      241 ctacagcgtc aatgtaggga ataacgtcac cttctcggtg ggcaagacga tgaaggaaaa
      301 caccggacaa acagcagttt attctgccgg tgagcatctt gaactctgct gtggtaaggc
      361 aaggctggtg ctgacgaagg acggaagcat atttctcaac ggtacgcaca ttcatctgga
      421 aggggagtcg gatgtgaacg gtgatgcgcc agtgattaac tggaactgtg gtgccacaca
      481 acctgtaccg gatgcgcctg tgccgaaaga tttacccccc ggaatgccgg atatgcggca
      541 attttgagtc aggtgcaaaa aattaaaacg gaactggtgc agtgaccaga tgaaccatat
      601 taaaagcaga aggaattgat tcatgggcgg cggaaaaccg gcggcgcggc agggcgacat
      661 gacccgcaag ggactggata ttgtgcaggg ttcagcgggt gtgctgatag gtgcgccgac
      721 gggagtggcc tgttcggtgt gtccgggagg gattacctat gctaacccgg tgaacccggt
      781 gaacccggtg ctgggtgcga aggtgcttcc gggcgagacg gaccttgcgc tgccctgccc
      841 gctgccgttt attcttttcc gtgcctacag cagctaccgg accagaacac ccgcgccggt
      901 gggggtgttt ggtcccggct ggaaagcgcc cttcgatatc cgcttacaga tacgcgatga
      961 aggcctgata ctcaacgaca gcggcggcag gagcattcac tttgagccgc tgtttcccgg
     1021 cgagataagc tacagccgca gcgagtcact ctggctggcg cggggcggag tggcggcgca
     1081 gcacagttcg cagccgctaa gcgcgctctg gcaggtgctg ccggaagatg ttcgcctgag
     1141 tccgcatgtg tacctggcga caaacagcct tcaggggccg tggtggatac tcagctggcc
     1201 ggagccgccc gcataccggg tactgacggt cgtggtggat ggcttcgggc gctcactgac
     1261 tttccaccgg gcggcggagg gtgacgtggc gggcgcggtg acgggggtga cggacggcgc
     1321 gggccgccgt tttcacatgg cgctgagcac gcaggcgcag cgggcggaag cgtcccgtaa
     1381 acagcgcgcc tcttccttat cttccccggc cagtccccgt tctgtttcct cttctcaggt
     1441 tttccccgac acgctgcccg ccggtacaga gtacggtgcc gataacggta tccggctgga
     1501 ggcggtatgg ctgacacacg acccggcata cccggatgaa cagccgactg cgccgctggc
     1561 gcgctacacg tacacggccg gtggtgaact gcgggcggtg tatgaccgca gcgggacgca
     1621 ggttcgcggg tttacttatg atgcggagca cgccgggcgg atggtggcgc accattatgc
     1681 ggggaggccg gagagccgct accggtatga tgataccggt cgggtgacgg agcaggtcaa
     1741 cccggagggg ctggactacc gctttgagta cggagagagc cgggtaatca tcacggacag
     1801 cctgaaccgg cgggaggtgc tgtacacgga aggcgagggt ggcctgaagc gggtggtgaa
     1861 gaaggagcat gccgacggga gcatcacccg cagcgagtat gatgaggcgg ggaggctgaa
     1921 ggcgcagacg gatgcggcgg ggcggcggac ggagtaccgg ctgcatatgg cgtctggaaa
     1981 gctgacttca gtggtactgc cggacggcag gacggtacgg tatggttaca acaaccagtt
     2041 gcaactgacg tcagtgacat atccggatgg tttacgcagc agccggaaat atgaccgtca
     2101 ggg
//
LOCUS       DAZPDU010000338         2093 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZPDU010000338 DAZPDU010000000
VERSION     DAZPDU010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.342"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..123)
                     /locus_tag="ACHURS_004576"
     CDS             complement(<1..123)
                     /locus_tag="ACHURS_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFR1240119.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPY"
     gene            384..719
                     /locus_tag="ACHURS_004577"
     CDS             384..719
                     /locus_tag="ACHURS_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFR1240120.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNSIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1000..1131)
                     /locus_tag="ACHURS_004578"
     CDS             complement(1000..1131)
                     /locus_tag="ACHURS_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFR1240121.1"
                     /translation="MIAYCFCEWHCSSRIMSVLADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(1631..1707)
                     /locus_tag="ACHURS_004579"
     tRNA            complement(1631..1707)
                     /locus_tag="ACHURS_004579"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1671..1673),aa:Arg,seq:tct)
     gene            complement(1808..1883)
                     /locus_tag="ACHURS_004580"
     tRNA            complement(1808..1883)
                     /locus_tag="ACHURS_004580"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1848..1850),aa:Ile,seq:cat)
     gene            complement(1986..>2093)
                     /locus_tag="ACHURS_004581"
     CDS             complement(1986..>2093)
                     /locus_tag="ACHURS_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR1240122.1"
                     /translation="YDALVTRFDIEEAWAERQLKKVTRLIFPESVVNSS"
BASE COUNT          677 a          522 c          370 g          524 t
ORIGIN      
        1 ataaggggtt atggccaccg gactaccaaa cggctgccag ccctctttca gtttatgtgt
       61 cagcttttcc gcaagatctg acggcggcgc cgccctgaca acatcatagt gtttaaatgc
      121 catggttctt tccaccatct gaaaaataat tctttaaaat acctgacatg taatacagaa
      181 aaaacacaaa accatacctt aaataaaaac ctgattatca agcagatatg catggataaa
      241 ctacaagacg agatataaac caccctgcat ttaaataaac aataaacaac atcagaaaaa
      301 taattctgct ctatggttta cattcaaaaa tatcatttat acttttcaga acatcaccag
      361 caaggcataa acaaggaaac taaatgaagt ggattgtgat tgatacagtt atccagccat
      421 catgcggaat atctttttca gtcatatgga gtaaaataaa attaataatc tggtatcaat
      481 cggatgcttt cttacctcct gaaagtatat ttacactgac tcacacaggc atcatgctca
      541 ataacaaagt gctacctgta accatttaca acgtagtacc attcaataaa acattctgga
      601 attcaatcaa aaatagccag gaatgcccta caaatacaga taacgtattg aatgaatgct
      661 ttaataaccg ttgcactctg caaatatgtc cttatggact aaaacaacaa agtccataag
      721 gagtttactc acatctgaca aaatcaatat aaacagcccc tccggagagg ggctggagag
      781 tggcgctatg tgccattgca tggtgccggg tgcctcccgg tgaattcagt accagcacct
      841 gaatccgcga ttatcccata tacctactcg ctgattgccc ctccgcacag ggggattcac
      901 catgccagtt tcttttaaca aactccccgc aaaccagaca acagtcaacc gcctgaattg
      961 tgaagtattt aaaaatttct cccgctaact gatacccggc taacagtctg gcgttttctt
     1021 tttcagcaac gggaaagcaa caaccaccac acccgccacc agcacaccgt cagccagcac
     1081 tgacattatc cggctgctgc aatgccattc acaaaaacag taagcaatca ctttttaccg
     1141 taacaggtga taatccagat atgtatctac cccagatgag taatccgaag ttcatccata
     1201 ccacaggtcc tggctattct gttgtactcc tgaacaagag caaataattc tgaattagca
     1261 accatgaact catcgcaaac cctctgtata gcatcactat tcagaataat aacgtctctt
     1321 cccgaaagac gatcaggagt acagaacaaa actgtcaaac ggctgaaggc ctttgctcgt
     1381 gctgcattga ctatatcaat acgctgccta aggatgaaac accccgacgc ctcatcaata
     1441 ttcactctac ccacaccata tgaatgataa atatttaatg ctgaaaaaac cattagaccg
     1501 tataacaaac actcaatcaa tacttaacag aacttttatt tttgacaaac ataaaatatt
     1561 ttcaacaata tcctgagcca ggtatatttc agtataaggc tctgccggaa ggaatctgga
     1621 agaatgaata tggcgcgctg tactggattc gaaccagtga ccgattgctt agaaggcaat
     1681 tgctctgtcc ggctgagcta acaacgctga ataccgataa tggaccgcca tcggggaccc
     1741 gcccccgcac caacaaccct gttatcatgt cgtctgctct tcctgataag ctaatggcgg
     1801 tttgtgatgg tggcccttgc tggatttgaa ccagcgacct ggcgattatg agtcgctcgc
     1861 tctcaccact gagctaaagg gccgggagca gaataataac ggtccgtaat taattccgca
     1921 ataaaaaacc cgctcggcgg cgggttgtag aaactcttct aacgtcaggc ataaaacgcc
     1981 catagttatg acgaatttac cacagattcc ggaaaaatca acctagttac cttttttaac
     2041 tgccgctcag cccatgcttc ttcaatatca aaccgggtca ccagcgcatc ata
//
LOCUS       DAZPDU010000339         2089 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZPDU010000339 DAZPDU010000000
VERSION     DAZPDU010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.343"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(60..1025)
                     /locus_tag="ACHURS_004582"
     CDS             complement(60..1025)
                     /locus_tag="ACHURS_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFR1240123.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRM
                     ASCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIV
                     VDYPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRG
                     KDGSAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGE
                     VVANVAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQL
                     DRETNEDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
     gene            complement(1879..>2089)
                     /locus_tag="ACHURS_004583"
     CDS             complement(1879..>2089)
                     /locus_tag="ACHURS_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240124.1"
                     /translation="RSEAVIPDRFKNLILLIDGLSFGTFGFHPHEGFEDELILYIQKT
                     NERVKTLFVKIDLNGIKFGFLRTHS"
BASE COUNT          577 a          550 c          436 g          526 t
ORIGIN      
        1 aaaaaaaata aaccaatagt cttatttcca ttcaggtatt caatattaat attcatacgt
       61 taatccgatt tatccttctc aggggcgcgt ttgtaacgcc gtttacctga atcccagaca
      121 aacgccgtcc gcttcccgtc atcagtgcca tcccctacat cgtcggcaat gacaagatcc
      181 tcattggttt ctcgatccaa ctggaggacg ttttttagcg ccccaccggt cgagataaaa
      241 cggcgcttgt gaagctgccg cgtcagctca agaaaccact caggatcgtt tgccatatct
      301 tccggtttaa cggagtattt cagcgtttca gcaaccgcgc tttgcagttg ctcggcaacg
      361 ttggccacaa cctcacctgt cttagttttt actgcccgaa tatcgatatt cggctcatag
      421 ttcacccgca agcaatcgcg ccagagttct acccaacgtt cgtgcttaac gtagttcttc
      481 cctttaaacc aagaaggttg caccatcagc agacagtgaa aatgcggatg tgcgctgcca
      541 tccttacctc gcgtcacctc cgtagccctg atccacccct gaacaggtga tagctccttt
      601 cgcttttcca ttcgcttaaa cgccgcattc attgctgtaa ggactgttcc aagttcacct
      661 atctcgcagt tcctgacagt taacgtcaga aacaaccatc gggaagacgg gtaatccaca
      721 acgattttcg gtagagcctg ataaaaacgg gcttgccaca tgagggttct tctccactgg
      781 cagacagggc aatgacggac acggcaaaat tgcgcactac gaagccgtag gcgcgtttct
      841 ccggtttcca tgatggtaga ccaaccaaaa cgtaacaaac cgctacagga ggccatacgg
      901 agtgcatagc gctcgaattc agcagcagtc tggtagatac ccccgacaga atcggtaagc
      961 gtgcgctggg tatcccaaac tgcatcacgg gggctgtagt ccgaaaggaa tttatcttct
     1021 gacattaagc ctgctccgat ctgggcaggc tgtgatttga tgtggtatat tcatcacgta
     1081 cctgtgcatt gcgagccagt acatgtcacc agagtttgcc gctgaggtga cacattcgaa
     1141 atgttgggga gtgttgacgc actccccaac gccgattaaa aacgcctcaa ttctgctcca
     1201 ttttctttga cctgtcaaaa cccactcaaa gcctcgtcat tactgggcta aaccactatt
     1261 tcatatccgc catttatccc ttaagtataa atacaaggat cacgcgcgag aattgcgcaa
     1321 aaaataacca caattttaac cccccaaaac aagcaccccc gaacggctta ccccaacctt
     1381 ttccacacac ccgatcacca aaacagggaa taaaaacaaa accctgtctt acaggtggat
     1441 ggcctcactt cgtgaaacca tccaccccgc ataagttttt taatcggtgg tgtcgtcaat
     1501 cactaaatcg gaaccccaaa ggggaccccc acgatttagc atttgacgag attaactctc
     1561 ggagatggca tcatgctttt aacggacttt aaaaaacggt gaacggatgc ggattttaat
     1621 ccgtgtctgt gaggcgttga gcaacgagca aagcgaggcg gtagaaagag agccagcggc
     1681 aggctcagga cgataatccg gggaaaatgt accactcgca gagtgttaac atttgacatc
     1741 ggattgtcta tcctgcattg gtgtcgagtt ggcaaggctg aggataatgc tgaggataat
     1801 gcaggataga cagagcacta gagattgcat gcaatctcgt acctatcacc tcagcctgcc
     1861 gctggctctt ttccagaact aagaatgcgt tcttaaaaat ccaaacttga taccgtttaa
     1921 atctattttc acaaaaagag tttttaccct ctcgtttgtt ttctgaatat ataaaattaa
     1981 ttcatcctca aaaccttcgt gcggatgaaa accaaatgtg ccaaaactta atccatcaat
     2041 aagcaaaata aggtttttaa acctgtcagg aatcaccgct tcacttctt
//
LOCUS       DAZPDU010000340         2063 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZPDU010000340 DAZPDU010000000
VERSION     DAZPDU010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.344"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..396
                     /locus_tag="ACHURS_004584"
     CDS             <1..396
                     /locus_tag="ACHURS_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000986822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240125.1"
                     /translation="EEMLSGEYAGFVYIFQFEDGSNYVGAKQIYQRVKDARKLKPTSK
                     ENGWREYLSSSNVVKNKIENGEQYSKTILWCFATMREVMIVEAILILHQMLKPECLNL
                     AMMSKIRSPNAKDKKRLLGIVQELLEYLN"
     gene            406..906
                     /locus_tag="ACHURS_004585"
     CDS             406..906
                     /locus_tag="ACHURS_004585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240126.1"
                     /translation="MASGINGIGSIRNYIQTQGVRLNNQFQNEIIKRSKLLSQRMQGD
                     LNNSVDRGAVGFTQRSILFFYRKVGRNSVTSTIMVKDIQAKYLYQVLVQPKAIDKFIP
                     TSSTRLTKQGNISGLKKNLLSERYKVVKGKNGKERLIDTAKKDTKKKTKRVIGLREKK
                     QRRLIY"
     gene            982..1293
                     /locus_tag="ACHURS_004586"
     CDS             982..1293
                     /locus_tag="ACHURS_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240127.1"
                     /translation="MNFEQHYGDLISKIKLNGMTPDGNSMPINKCFLDKKFQKVVKKS
                     NYQIDSYMNVIYEKKKDFKVGEVLEWELQGEKMPVFLIESKKLFVKGKHFWVYAVGII
                     E"
     gene            1290..1583
                     /locus_tag="ACHURS_004587"
     CDS             1290..1583
                     /locus_tag="ACHURS_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240128.1"
                     /translation="MISILIEFLKSGLDFFIKKKTIEQEVKKTNVEGQIETNKEEIEK
                     VTFHWRNALGFVLTLIILYNWIIVPVLDAFGIIVIQVPLGQLLQVLLIMVGGS"
     gene            1644..>2063
                     /locus_tag="ACHURS_004588"
     CDS             1644..>2063
                     /locus_tag="ACHURS_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR1240129.1"
                     /translation="MSDAITTAAIKKLYGLSAGQCNICRTSLFKEQVHIGQMAHVIAK
                     SPKGPRGDDLLANDNSYENLILLCANDHILVDRDPFTYTVEKLHQIKSEHEAYIQGAT
                     DNTLFSDKKRQSDVDFLNAYFHFTPFARVRSLVETLPN"
BASE COUNT          725 a          294 c          445 g          599 t
ORIGIN      
        1 gaggaaatgt tatccggtga atatgctggt ttcgtttata tctttcagtt tgaggatggt
       61 tctaattatg tcggtgctaa gcaaatctat cagcgggtta aggatgcgag aaagctaaag
      121 cccacttcaa aggaaaatgg ctggcgtgag tacctcagta gttccaatgt cgttaaaaac
      181 aaaattgaaa atggtgaaca gtactcaaaa acgattctat ggtgcttcgc aactatgcga
      241 gaggtaatga ttgtagaggc aatactgatc cttcatcaga tgcttaaacc ggaatgtttg
      301 aatctggcaa tgatgagtaa gattagatca ccaaatgcaa aggataaaaa aagactactc
      361 ggaatagtac aggaactatt ggagtacttg aattgaggta aatatatggc aagtggaata
      421 aatgggattg gtagtataag gaactatata caaacgcagg gagttagatt aaataatcaa
      481 ttccagaatg aaataattaa acgttccaag ctactatccc aaagaatgca gggtgatttg
      541 aataatagcg ttgaccgtgg ggcagtgggc tttactcaac gttccatcct tttcttctat
      601 agaaaggttg ggagaaattc agttacatcg accatcatgg ttaaagatat tcaggctaag
      661 tacctttatc aagtactggt acagccaaag gcaattgata agtttatacc tacttcatct
      721 acaagattaa ctaagcaggg caacatttca ggactgaaaa agaatctttt aagtgaacga
      781 tataaggtag tgaagggtaa gaatggtaaa gaacgattaa ttgataccgc aaagaaagat
      841 accaagaaga aaaccaaacg tgtaattggc ttacgtgaga agaaacaacg tcgccttatc
      901 tattgacttc tataaggaag ccaatgatgg tgtaagggta attatgagcg gtattcaggg
      961 aacattcagg ataagtagac aatgaatttt gagcaacatt atggtgattt aataagcaag
     1021 attaagctaa atggcatgac accagatggt aatagtatgc caattaacaa atgttttctt
     1081 gataagaaat ttcaaaaggt cgttaagaaa agtaattatc agattgatag ctatatgaac
     1141 gttatttatg agaagaagaa agatttcaaa gttggtgaag tacttgaatg ggaattacag
     1201 ggtgaaaaga tgcctgtatt tctaattgag agtaaaaagc tctttgtgaa gggtaaacat
     1261 ttttgggtat atgccgtggg gattattgaa tgattagtat attgattgag tttctgaagt
     1321 caggattgga ttttttcatt aagaagaaaa ctattgaaca ggaagtaaag aaaacgaatg
     1381 tagaagggca gattgaaacg aataaagaag agattgaaaa ggtaacattc cactggcgta
     1441 atgccctcgg ttttgtacta acccttatca ttctctataa ctggatcata gtaccagtac
     1501 ttgatgcttt tggaattatt gtgatccaag ttccattagg acaacttttg caagtgctat
     1561 tgattatggt tggtggaagc tgaaagattt ttcctttaat atgtgttttg agttaataaa
     1621 atagcaatct aaggatttga tttatgagtg atgcaattac tacagcagca ataaaaaaat
     1681 tgtatggact atccgctggg caatgtaata tttgccgaac ttctcttttt aaagagcagg
     1741 ttcacatcgg acaaatggct catgtaatag ccaaaagccc aaaaggacca aggggcgatg
     1801 acctgttagc aaatgacaat tcttatgaaa atcttatcct gctttgtgca aatgatcata
     1861 ttcttgttga tagagatcca ttcacttata cagtagaaaa attgcatcaa atcaaaagtg
     1921 aacatgaggc ttatatacag ggtgcaaccg ataatacatt attttcagat aaaaaaagac
     1981 agtcagatgt ggacttttta aacgcttact tccactttac accgtttgcg agggtacgtt
     2041 cattagttga aactttacct aat
//
LOCUS       DAZPDU010000341         2014 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZPDU010000341 DAZPDU010000000
VERSION     DAZPDU010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.345"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(136..606)
                     /gene="argR"
                     /locus_tag="ACHURS_004589"
     CDS             complement(136..606)
                     /gene="argR"
                     /locus_tag="ACHURS_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFR1240130.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            1041..1979
                     /gene="mdh"
                     /locus_tag="ACHURS_004590"
     CDS             1041..1979
                     /gene="mdh"
                     /locus_tag="ACHURS_004590"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFR1240131.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
BASE COUNT          522 a          438 c          519 g          535 t
ORIGIN      
        1 ttaccgtatg tattcattgt gtgaatgata tgtcgcggta aaacgcacta ttcgttaaat
       61 taatcgcagg ggaaagcagg gggttgagag gaataagcaa cattttcccc gccgccagaa
      121 gcgacggggc agagattaaa gctcctggtc gaacagctct aaaatcgctt cgtacaggtc
      181 tttgacggtg aaaccgttag caggggtggt aaagatggtg tcatcgccag cgatggtgcc
      241 cagaatacct tctgctttgc ccagtgagtc cagcaggcga gcaattaact gcgccgcgcc
      301 agggctggta tgaatcacga caactgcatc gttgtagtcg atatccagca ccagattctt
      361 caatggactg gaggtggttg gtacacccag ttcagctggc aggcagtaaa ccatttccat
      421 tttggcattg cgtgtacgta cagcaccaaa cttggtcaac atccgcgaga ctttagactg
      481 attaatattg tcaaagcctt gctcctgcaa cgcggcgacg atttcgccct gggagctaaa
      541 tttctcttct ttaagtaatg ctttaaatgc tttaactagt tcttcttgct tagccgagct
      601 tcgcataagt cacccgatat ggtggttgat acaacattat tgtgcataca gatgaatttt
      661 tatgcaaaca gtcagccctg aagaaggctg aaataatgtt atgaaagagc gggattttat
      721 caaatttcgt tattgagaaa catgcctgcg tcacggcatg caaattctgt ttaaaagtaa
      781 attaattgtt atcaaattga tgttgttttg gctgaacggt agggtatatt gtcaccacct
      841 gttggaatgt tgcgctaatg cataagcgac tgttaattac gtaagttagg ttcctgatta
      901 cggcaattaa atgcataaac gctaaacttg cgtgactaca cattcttgag atgtggtcat
      961 tgtaaacggc aattttgtgg attaaggtcg cggcagcgga gcaacatatc ttagtttatc
     1021 aatataataa ggagtttagg atgaaagtcg cagtcctcgg cgctgctggc ggtattggcc
     1081 aggcgcttgc actactgtta aaaacccaac tgccttcagg ttcagaactc tctctgtatg
     1141 atatcgctcc agtgactccc ggtgtggctg tcgatctgag ccatatccct actgctgtga
     1201 aaatcaaagg tttttctggt gaagatgcga ctccggcgct ggaaggcgca gatgtcgttc
     1261 ttatctctgc aggtgtagcg cgtaaaccgg gtatggatcg ttccgacctg tttaacgtta
     1321 acgccggcat cgtgaaaaac ctggtacagc aagttgcgaa aacctgcccg aaagcgtgca
     1381 ttggtattat cactaacccg gttaacacca cagttgcgat tgctgctgaa gtgctgaaaa
     1441 aagccggtgt ttatgacaaa aacaaactgt tcggcgttac cacgctggac atcattcgtt
     1501 ccaacacttt tgttgcggaa ctgaaaggca aacagccagg cgaagttgaa gtgccggtta
     1561 ttggcggtca ctctggtgtt accattctgc cgctgctgtc acaggttcct ggcgttagtt
     1621 ttaccgagca ggaagtggct gatctgacca aacgcatcca gaacgcgggt actgaagtgg
     1681 ttgaagcgaa agccggtggc gggtctgcaa ccctgtctat gggccaggca gctgcacgtt
     1741 ttggtctgtc tctggttcgt gcactgcagg gcgaacaagg cgttgtcgaa tgtgcctacg
     1801 ttgaaggcga tggtcagtac gcccgtttct tctctcaacc gctgctgctg ggtaaaaacg
     1861 gcgtggaaga gcgtaaatct atcggtaccc tgagcgcatt tgaacagaac gcgctggaag
     1921 gtatgctgga tacactcaag aaagatatcg ccttgggcga agagttcgtt aataagtaat
     1981 tgattagcga ataataaaaa accggagcac agac
//
LOCUS       DAZPDU010000342         2007 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZPDU010000342 DAZPDU010000000
VERSION     DAZPDU010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.346"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            1..988
                     /locus_tag="ACHURS_004591"
                     /pseudo
     CDS             1..988
                     /locus_tag="ACHURS_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
     gene            999..1436
                     /locus_tag="ACHURS_004592"
                     /pseudo
     CDS             999..1436
                     /locus_tag="ACHURS_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1436..1813
                     /locus_tag="ACHURS_004593"
     CDS             1436..1813
                     /locus_tag="ACHURS_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240132.1"
                     /translation="MNQYIASYIEKMASFSTVLSETVSLANEYWHPETPPVVMLFSQV
                     GKTIVKNFYEMGDIEREKIFKHVEDGMVSLDDELATVVATGLIEAIVTATDSNSLLWQ
                     DLSKFLGDKSKSHALVWKNFGQE"
BASE COUNT          660 a          323 c          503 g          521 t
ORIGIN      
        1 tggggaaaca catctcgatt ttttgaagca atatggtgat tttgaaaatg gtattcctgt
       61 tcacgatacc attgccagag ttgtatcctg tatcagtcct gcaaaatttc acgagtgctt
      121 tattaactga atgcgtgact gccattcttc agatgataaa gacgtcattg caattgatgg
      181 aaaaacgctc cggcactctt atgacaagag tcgccgcagg ggagcgattc atgtcattag
      241 tgcgttctca acaatgcaca gtctggtcat cggacagatc aagacggatg agaaatctaa
      301 tgagattaca gctatcccag aacttcttaa catgctagat attaaaggaa aaatcatcac
      361 aactgatgcg atgggttgcc agaaagatat tgcagagaag atacaaaaac agggaggtga
      421 ttatttattc gcggtaaaag gaaaccaggg gcggctaaat aaagcctttg aggaaaaatt
      481 tccgctgaaa gaattaaata atccagagca tgacagttac gcaatgagtg aaaagagtca
      541 cggcagagaa gaaatccgtc ttcatattgt ttgcgatgtc cctgatgaac ttattgattt
      601 cacgtttgaa tggaaaggac tgaagaaatt atgcgtggca gtctcctttc ggtcaataat
      661 agcagaacaa aagaaagagc cagaaatgac ggtcagatat tatatcagtt ctgctgattt
      721 aaccgcagaa aagttcgcca cagcaatccg aaaccactgg cacgtggaga ataagctgca
      781 ctggcgtctg gacgtggtaa tgaatgaaga cgactgcaaa ataagaagag gaaacgccgc
      841 agaattattt tcagggatac ggcacatcgc tattaatatt ttaacgaatg ataaggtatt
      901 caaggcaggg ttaagacgta agatgcgaaa agcagccatg gatagaaact atctcgcgtc
      961 agtccttgcg gggagcgggc tttcgtaatc tttccctagg gagtcagccg aatgttggga
     1021 aggatttgac ggatgaagag aagaactgct tggccggcgg cagtacggga agcccgggag
     1081 gatggggacc agaagatgag aatagcgcaa gggatcgtat gcagcatgaa cggtataaag
     1141 atgaattgcg acagaaaatg ggaaaacccg acgttacaga ttcaaatttg aaaaatatta
     1201 tagacgatct ttatcggcca aatgcaaaag taggaagtgg aagcacagca gatgctgttc
     1261 gttatgaatt ggccacaggt gaaaaagttg gtggtagagg acatgtattg aaagcacagg
     1321 attatacaag ggctttacag gattggttgg ataagaatcc aacagcatct ccaggggatc
     1381 gagcagcagc agaaaacgtt cttcaggatt tacagaatgc tttgggaggc aagtaatgaa
     1441 ccaatatatt gctagttata tagaaaaaat ggcatcattt tccactgttt tgtctgagac
     1501 agtctcatta gctaatgaat actggcatcc agaaacgcca ccggtggtta tgttgttctc
     1561 tcaggttgga aaaacgattg taaaaaattt ttatgaaatg ggggatattg aaagagaaaa
     1621 aatatttaaa catgtcgaag atggtatggt ctcgcttgat gatgaattgg ccacagttgt
     1681 agctacaggg cttatagagg caatagtcac tgcgaccgat agcaattctt tactttggca
     1741 agatttaagt aaatttctgg gtgacaaatc aaagtctcat gcattagtgt ggaaaaattt
     1801 tggtcaggag tgatattatt caagaagtat taagctcgct attgagatct taatgcttct
     1861 tgagattaaa ttaaagttag gacgaagtaa ttgcaggtgg aaataaatcc atagtgacgt
     1921 gtggcaatgg ctattagatg ttatggtgtg atgggctttg gtgatttaag agctaagtta
     1981 gattgcctta tgaaaaagta tggttat
//
LOCUS       DAZPDU010000343         2005 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZPDU010000343 DAZPDU010000000
VERSION     DAZPDU010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.347"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..389
                     /locus_tag="ACHURS_004594"
     CDS             <1..389
                     /locus_tag="ACHURS_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240133.1"
                     /translation="FDLSLEIPLPPPGILSKTVVPGESSATVKQRVMAARERQFKRQN
                     KLNAWLDSPEIRQFCKLESEDAMWLEGTLIHLGLSIRAWQRLLKVARTIADIDQSDII
                     TRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(414..752)
                     /gene="maoP"
                     /locus_tag="ACHURS_004595"
     CDS             complement(414..752)
                     /gene="maoP"
                     /locus_tag="ACHURS_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFR1240134.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            871..1710
                     /gene="hdfR"
                     /locus_tag="ACHURS_004596"
     CDS             871..1710
                     /gene="hdfR"
                     /locus_tag="ACHURS_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFR1240135.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(1806..1881)
                     /locus_tag="ACHURS_004597"
     tRNA            complement(1806..1881)
                     /locus_tag="ACHURS_004597"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1846..1848),aa:Trp,seq:cca)
     gene            complement(1890..1966)
                     /locus_tag="ACHURS_004598"
     tRNA            complement(1890..1966)
                     /locus_tag="ACHURS_004598"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1930..1932),aa:Asp,seq:gtc)
BASE COUNT          529 a          484 c          487 g          505 t
ORIGIN      
        1 gcttcgatct ctcactggag atcccattac caccccccgg cattttgagt aaaacggtag
       61 tgccgggaga aagcagcgcc accgttaaac aacgcgtaat ggccgccaga gagcgccaat
      121 ttaagcggca gaataaactg aatgcctggc tggatagtcc ggaaatacgc caattctgca
      181 aacttgagag cgaagatgcg atgtggttgg aaggaacact gatccatctg gggttatcga
      241 ttcgtgcctg gcagcggtta ttgaaagttg cacgaaccat tgctgatatt gatcagtctg
      301 acattatcac acgtcagcat ttgcaggagg cagttagcta tcgtgcgatt gaccgtttgc
      361 tcatccatct gcaaaaacta ctgacataaa aaaagggcat ttcgcccttt ttattaatca
      421 tcagaatcgg tgtagtcttc agcaccttca acctgcggtt taccgccgga aagggtgtga
      481 aaacgttttg gacgcttaat acgcgtcata tacttggacc acacgcgttc tgcttctgtc
      541 actggctcac gttcgccacg gcatactgct acgaagagtt tctcttcctc ggtaaccggc
      601 tcgcgtttgc caagatccaa ctcattgaag gcataaccat gacgctcaag cagttgtgcc
      661 tctttgatgg tgaaatcacc atgacgagag aatccacgtg gataatgttt attgtcgaaa
      721 tatcgattag tcgtcgtaaa gctttccgcc atcctgcacg ctcctaattc tttgaccgag
      781 ctagttatgg cgcggagtat tagttacgct tgacagagtg taaaacaaaa catttaaatc
      841 ataacgacaa ataattttgc ggagagcact gtggatacgg aattgttaaa aactttcctg
      901 gaagttagcc gaacgcgtca ctttggtcga gcggctgaat cgctctatct gacccagtca
      961 gcagtgagct ttcgaatcag gcaactggaa aatcaactgg gtgtgaacct tttcacccgc
     1021 cacagaaaca atatccgttt aaccgctgcc ggtgaaaaac tactgcctta tgcagaaacg
     1081 ctcatgagca cctggcaggc cgcccgcaag gaggtggcgc atacctcacg acataacgag
     1141 ttttctatcg gtgccagcgc ctcgttgtgg gaatgtatgc ttaatcagtg gttgggacgc
     1201 ttgtatcaaa atcaggatgc ccatacaggc ttacagttcg aagcgcgaat tgcccaacgg
     1261 cagtctctgg taaaacagct gcatgaacgc cagcttgatc ttcttattac cactgaagcg
     1321 cccaaaatgg acgaatttag tagtcagttg ctggggtatt tcactttagc gctttatacc
     1381 agtgcccctt ctaaactaaa gggagatctt aactatctgc gactggagtg ggggccagat
     1441 tttcaacagc atgaggcagg tttgatcggt gctgacgaag ttcccattct gacaaccagt
     1501 tctgctgaac tggcacagca acagattgcg atgcttaatg gttgcacctg gctacccgtt
     1561 agctgggcgc gtaaaaaagg cggcctgcat accgttgtcg atagcacaac actttcacgg
     1621 ccgctttatg ccatatggct gcaaaatagc gataaaaatg cgttgattcg cgatcttttg
     1681 aaaattaacg tgctggatga agtgtattaa tctgaatggc tggcaaggat gccggtagaa
     1741 ggatttactt cggagaggat tatttcagat aaaaaaaatc cttagctttc gctaaggatg
     1801 atttctggca ggggcggaga gactcgaact cccaacaccc ggttttggag accggtgctc
     1861 taccaattga actacgcccc taattagggt ggcggaacgg acgggactcg aacccgcgac
     1921 cccctgcgtg acaggcaggt attctaaccg actgaactac cgctccaccg aattctttta
     1981 caaccaccgg ttttatgacc ggctt
//
LOCUS       DAZPDU010000344         2002 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZPDU010000344 DAZPDU010000000
VERSION     DAZPDU010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.348"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..314
                     /locus_tag="ACHURS_004599"
     CDS             <1..314
                     /locus_tag="ACHURS_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240136.1"
                     /translation="ALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYS
                     GAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDSQR
                     A"
     gene            416..1870
                     /gene="amn"
                     /locus_tag="ACHURS_004600"
     CDS             416..1870
                     /gene="amn"
                     /locus_tag="ACHURS_004600"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFR1240137.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWNLITADAQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIDIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT          479 a          496 c          476 g          551 t
ORIGIN      
        1 tggcgcttga agcacaatct attttctgga tagttttctt ctccataatg ctggcaaaca
       61 ttgcgcatga catggtggtg tgtgtgcaac aaccgatgtt taccgaaatg tttggtgcca
      121 gttatcgcta tagtggcgct ggagtcggtt atcaggttgc cagtgtggtt ggcggtggat
      181 ttacaccttt tattgccgct gcactcatca cttactttgc cgggaactgg catagcgtcg
      241 ccatttattt gctggctgga tgcctgattt ccgcaatgac cgctttgttg atgaaagaca
      301 gtcaacgcgc ttgatagcct ggcgaagatc atccgatctt cgccttacac ttttgtttca
      361 catttctgtg acatactatc ggatgtgcgg taattgtatg gaacaggaga cacacatgaa
      421 taataagggc tccggtctga ccccagctca ggcactggat aaactcgacg cgctgtatga
      481 gcaatctgta gtcgcattac gcaacgccat tggcaactat attacaagtg gcgaattacc
      541 tgatgaaaac gcccgcaaac aaggtctttt tgtctatcca tcactgaccg taacctggga
      601 cggtagcaca accaatcccc ccaaaacgcg cgcatttggt cgttttactc acgcaggcag
      661 ctacaccacc acgattactc gccctactct ctttcgttcg tatcttaatg aacaacttac
      721 gttgctgtat caggattatg gtgcgcatat ctctgtgcaa ccctcgcagc atgaaatccc
      781 ttatccttat gtcatcgatg gctctgaatt gacacttgat cgctcaatga gcgctgggtt
      841 aactcgctac ttcccgacaa cagaactggc gcaaattggc gatgaaactg cagacggcat
      901 ttatcatcca acggaattct ccccgctatc gcattttgat gcgcgccgcg tcgatttttc
      961 cctcgcacgg ttgcgccatt ataccggtac gccagttgaa cattttcagc cgttcgtctt
     1021 gtttaccaac tacacacgtt atgtggatga attcgttcgt tggggatgca gccagatcct
     1081 cgatcctgat agtccttaca ttgccctttc ttgtgctggc gggaactgga tcaccgccga
     1141 aaccgaagcg ccagaagaag ccatttccga ccttgcatgg aaaaaacacc agatgccagc
     1201 atggaattta attaccgccg atgctcaggg tattactctg gtgaatattg gcgtgggacc
     1261 atcaaatgct aaaaccatct gcgatcatct ggcagtgcta cgcccggatg tctggttgat
     1321 gattggtcac tgtggcggat tacgtgaaag tcaggccatt ggcgattatg tacttgcaca
     1381 cgcttattta cgcgatgacc acgttcttga tgcggttctg ccgcccgata ttcctattcc
     1441 gagcattgct gaagtgcaac gtgcgcttta tgacgccacc aagctggtga gcggcaggcc
     1501 cggtgaggaa gtcaaacagc ggctacgtac tggtactgtg gtaaccacag atgacaggaa
     1561 ctgggaatta cgttactcag cttctgcact tcgttttaac ttaagccggg ccgtagcaat
     1621 tgatatggaa agtgcaacca ttgccgcgca aggatatcgt ttccgcgtgc catacgggac
     1681 actactgtgt gtttcagata aaccattaca tggtgagatt aaacttcccg gtcaggctaa
     1741 ccgtttttat gaaggcgcta tttccgaaca tctgcaaatt gacattcggg cgatcgattt
     1801 gctgcgcgca gaaggcgacc gactgcattc gcgtaaatta cgaaccttta atgagccgcc
     1861 gttccgataa taagttctga accttcttat cagaaataga tgtgaaggag taagtaagac
     1921 acctggcaaa tagcctgcaa taatcgtggg ctgtttgctt ccttgggcgg atacgagttt
     1981 tattatcgtc ttaatgattt cc
//
LOCUS       DAZPDU010000345         1997 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZPDU010000345 DAZPDU010000000
VERSION     DAZPDU010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.349"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..476
                     /gene="istB"
                     /locus_tag="ACHURS_004601"
     CDS             <1..476
                     /gene="istB"
                     /locus_tag="ACHURS_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS21-like element ISSso4 family helper ATPase
                     IstB"
                     /protein_id="HFR1240138.1"
                     /translation="YIIHRKNLLITGPTGCGKSWIANALGEQACRQKYSVRYCRTGRL
                     LEQLAQGRVDGSWLKYLKQLQKIQVLILDDLGLEQLSNAQCNDLLEITEDRYGQSSTI
                     VVSQFPVDKWHGLMENPTTADAILDRLVHNSHRVVLQGESLRKNPPTVESSEKTS"
     gene            565..1997
                     /gene="rhsC"
                     /locus_tag="ACHURS_004602"
                     /pseudo
     CDS             565..1997
                     /gene="rhsC"
                     /locus_tag="ACHURS_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsC"
BASE COUNT          522 a          545 c          595 g          335 t
ORIGIN      
        1 actacataat ccacaggaaa aacctactga taaccggtcc gacaggatgc ggtaagagct
       61 ggatagccaa tgcgctgggc gaacaggcat gtcgacagaa atacagcgtc cggtactgta
      121 gaaccgggcg attactggaa cagttggctc agggacgtgt tgatggcagt tggctgaaat
      181 atctgaaaca gttgcaaaaa atacaggttc taatcctgga cgatctgggg ctggaacagt
      241 taagtaacgc ccagtgtaat gaccttctgg agatcacaga ggatcgttac ggacaaagca
      301 gcacgatagt ggtaagccag ttcccggtag ataaatggca tggtctgatg gagaacccaa
      361 caacagcaga tgccatcctg gacagactgg tacataactc acacagagtg gtccttcagg
      421 gagagtccct gaggaaaaat cccccgacag tggaaagcag cgaaaaaacg agttaaaaag
      481 cggtagaaaa agttaagttc ccggttgatc gaatggatgc gaatcgctga tcgaacttcg
      541 atgccaatcc gtgatcgaat agagcggaat acgcagctgg tactgaccac gcaggcgcag
      601 cgggcagaag aagcccggca gcaggccatt tccggcggga cggaaccgtc cgcttttcct
      661 gataccctgc cgggttacac cgaatatggc cgggacaacg gcatccgtct gtctgccgtg
      721 tggctgacgc acgacccgga atacccggag aatttacctg ccgcgccgct ggtgcgctat
      781 ggctggacgc cgcgcggcga actggcggca gtgtatgacc gcagcaatac acaggtgcgc
      841 agctttactt acgatgataa ataccggggc cggatggtgg cgcaccgtca cacgggccgg
      901 ccggaaatcc gttaccgtta cgacagcgac gggcgggtga cagaacagct aaacccggca
      961 ggcttaagct acacgtatca gtatgagaag gaccgcatca ccatcaccga cagcctgaac
     1021 cgccgtgaag tgctgcacac gcagggtgaa gccgggctga agcgggtggt gaaaaaggaa
     1081 cacgcggacg gcagcgtcac gcagagtcag tttgatgcgg tgggcaggct cagggcacag
     1141 acggatgccg caggccggac gactgagtac agcccggatg tggtgacggg actcatcacg
     1201 cgcatcacca cgccggatgg cagggcatcg gcgttttact ataaccacca cagccagtta
     1261 acgtcagcca ccgggcctga cgggctggaa atgcgccgca aatatgatga atccggccgt
     1321 ctgattcagg aaactgcccc tgacggcgat atcatccgct accgttatga taatccacac
     1381 agtgacttac cctgcgcaac ggaagatgcc accggcagcc ggaaaaccat gacgtggagc
     1441 cgttacggtc agttgctgag cttcaccgac tgttccggtt atgtaacccg ttatgaccat
     1501 gaccgcttcg ggcagatgac ggcggtgcac cgcgaggaag ggctgagtca gtaccgcgca
     1561 tacgacagcc gtggacagtt aattgccgtg aaagacacgc agggccatga aacgcggtat
     1621 gaatacaacg ccgccggtga cctgaccacc gtcattgccc cggacggcag cagaaacggg
     1681 acacagtacg atgcgtgggg aaaagctatc tgtaccacgc agggcggtct gacgcgcagt
     1741 atggaatacg atgctgccgg acgggtcatc cgcctgacca gtgaaaacgg cagccacacc
     1801 accttccgtt acgatgtact cgacctgctg atacaggaaa ccggctttga cggccgcaca
     1861 cagcgttatc accacgacct gaccggcaaa cttatccgca gcgaggatga ggggctggtc
     1921 acccactggc actatgacga agcagaccgc ctcacgcacc gcaccgtgaa tggcgaaacc
     1981 gcagagcggt ggcagta
//
LOCUS       DAZPDU010000346         1896 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZPDU010000346 DAZPDU010000000
VERSION     DAZPDU010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.350"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            640..987
                     /locus_tag="ACHURS_004603"
     CDS             640..987
                     /locus_tag="ACHURS_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
                     /protein_id="HFR1240139.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTVLTGGLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLSGLLMASFHWW
                     RRKVNRLPPWDDV"
     gene            complement(1007..1597)
                     /locus_tag="ACHURS_004604"
     CDS             complement(1007..1597)
                     /locus_tag="ACHURS_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240140.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLEQSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRIRAEQR
                     AAEVVVLPLVPAAGLPAGGTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     WVVRREGDPLEVAQWLQACHDAGMEVRVQINESAAP"
     gene            complement(1594..1854)
                     /locus_tag="ACHURS_004605"
     CDS             complement(1594..1854)
                     /locus_tag="ACHURS_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240141.1"
                     /translation="MDQEMTFSLSYEQLTRFAEKRIRECNLDSHGVTYLCESAKAGAV
                     LIFWHELAINGYTSMNAIKRQEIIDADHQRLRKLIWPEDDWK"
BASE COUNT          458 a          508 c          453 g          477 t
ORIGIN      
        1 caatatgtgc attatcatat aatcttacag cgcaagcttc cccgtcatac agacctcgtg
       61 atcgagttaa taagactatg atagaactac aaactaacgg agtagaacgg caacttttag
      121 ataattcttg tggcataagt tctggggtat ttaaataaaa tataagacag gctgtctatc
      181 ttacagacag cctttttata ataacagaaa gaatatatat catacttaac ggaagagata
      241 atatgaaaac agtacccgta taaacagcaa gcaatcccat ttttccaggt gtatcagcaa
      301 aaaaaccgct gttctagaaa tcaggccggg taaatttcag agctgtatct tcaatatacc
      361 attttgcaac cggatacaaa accattccgc agagagaaat acaccagaac agtaatctgt
      421 atttaaaatc ataatcccat gacatataca gcatatatcc tcccgtcacc catccccacc
      481 acatattatt aaaataatat tttctgttca tctgccagtt ttcctttctt ttgcactttt
      541 gtcagtgtac tgatgcatga caacataacc acagcatgat aataataatc aataacaata
      601 agctgtgtca cgtttacatc atggaacgga ggtgctcctg tgacttttga gcagaaaaaa
      661 gcccgggcca ttgccctgat ggacagcaaa aagatgtggc ggagcaatta tgcccctcct
      721 cttctgcgta ttctctggcg gctgggtatc cggcttccgc cattaccctt tatgccgttc
      781 tggcaggtca cggtgctgac ggggggctta tggggcattt cctggggatg tgcaatgtgg
      841 tttatctact gggggccgtc aggaatggtg gcaggtgagg ccattattat aagtatcacg
      901 gggggttttt tgtccggact gctcatggcc tctttccact ggtggcgcag gaaagttaac
      961 cgactgccac catgggatga tgtgtaaacc gtgcacaggc gtgttcttac ggagcggcac
     1021 tttcatttat ctgtacgcga acctccattc ctgcatcgtg acaggcctgc agccactgcg
     1081 ccacttccag cggatcgccc tcccggcgta ccacccagcc ttctttattc cataactgca
     1141 gacaggtgct gccgtcgaga cgcaccacaa aatccccacg gcaggcctga tacgttatgc
     1201 cgttcagctc caccgccaga tcagtggcag aagtgccgcc tgcgggcaga cctgctgccg
     1261 ggaccagcgg cagtacgacg acttctgccg cacgctgttc tgcccggatg cggtcctgtt
     1321 ctgccagcag cagaggctgt gccacggccc ggccggcgtc cgtcagctcc actgccagct
     1381 gcaggttcgg agcgcgcagg gtacgcagcc agccggcatc ctccagacga cggcaggagg
     1441 cgcgcaggtt tggcccgtag accggggctt ccccgctttg ctccagcacc cgttctatgt
     1501 cccgcgtcag cacgggtcct ggccgttttt catcaagggc agccagcaca atcagcactc
     1561 tccgctgcag tggcgacggt cggcggctct gagtcatttc cagtcatcct ccggccagat
     1621 gagctttctg aggcgctgat ggtcggcgtc aatgatctcc tgtctcttta tggcattcat
     1681 tgaggtatag ccatttatgg ccagttcatg ccagaagatc agcacggctc cggctttggc
     1741 tgactcgcag agataagtta cgccgtggct gtccagattg cattcccgga tgcgtttttc
     1801 cgcaaaccgg gtcagttgtt catagctgag tgaaaacgtc atttcctgat ccatcctgca
     1861 cgttccatct gataatgttc tgacaggtaa aaatca
//
LOCUS       DAZPDU010000347         1848 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZPDU010000347 DAZPDU010000000
VERSION     DAZPDU010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.351"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..>1848
                     /locus_tag="ACHURS_004606"
     CDS             <1..>1848
                     /locus_tag="ACHURS_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFR1240142.1"
                     /translation="GFPPSHAGTITVYEDSQPGTLNDFLGAMTEDDARPEALRRFELM
                     VEEVARNASAVAQNTAEAKKSASDASTSAREAATHATDAAGSARAASTSAGQAASSAQ
                     SASSSAGTASAKATEASKSAAAAESSKSAAATSAGAAKTSETNAAASQQSAATSASTA
                     TTKASEAATSARDASASKEAAKSSETNAASSASSAASSATAAGNSAKAAKTSETNAKA
                     SETAAEQSASAAAGSKTAAASSASAASTSAGQASASATAAGKSAESAASSASTATTKA
                     GEATEQASAAASSASAAKTSETNAKASETSAESSKTAAASSASSAASSASSASASKDE
                     ATRQASAAKSSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAEDIASAVA
                     LEDASTTKKGIVQLSSATNSTSESLAATPKAVKAAYDLADGKYTAQDATTAQKGIVQL
                     SSVTNSTSETLAATPKAVKTAYDNAEKRLQKDQNGADIPGKDTFTKNIGACRAFGGSV
                     STTTGNWTTAQFIEWLDSQGAFNHPYWMCKGSWSYGNNKIITDTGCGNIHLAGAVIEV
                     MGIKSAMTIRITTPTTSSGGGTTNAQFTYINHGTDYSPGWRRDYNSRNKP"
BASE COUNT          519 a          523 c          526 g          280 t
ORIGIN      
        1 ggattcccgc cgtcacatgc cgggaccatc accgtgtatg aagattctca acccggtacg
       61 ctgaatgatt ttctcggtgc catgacggag gatgatgccc gtccggaggc actgcgacgt
      121 tttgaactga tggtggaaga ggtggcgcgt aacgcgtccg cggtggcaca gaacacagca
      181 gaagcgaaga agtcagccag cgatgccagc acatcagccc gtgaggcggc aacccatgcg
      241 actgatgctg caggctcagc acgcgcagcc agcacgtcag ccggacaggc cgcgtcgtcg
      301 gctcagtcag cgtcttccag cgcaggaacg gcatcagcaa aggccactga agcatcaaaa
      361 agtgctgccg ctgcagagtc ctcaaaaagc gcggcggcta ccagtgccgg tgcggcgaaa
      421 acgtcagaaa cgaatgcggc agcgtcacaa caatcagcag ccacttctgc atccaccgcg
      481 accacgaaag cgtcagaagc agccacttca gcacgggatg cgtcggcttc aaaagaggcg
      541 gcaaaatcat cagaaacgaa cgcagcctcg agcgccagca gcgcagcttc ctcggctacg
      601 gcggcaggaa attccgcgaa ggcggcaaaa acgtccgaga cgaacgccaa ggcttctgaa
      661 acggcagcgg aacagagtgc ctccgcagca gcaggctcaa aaacagcggc tgcgtcgtct
      721 gccagtgcag cgtcaacaag tgccgggcag gcctcagcca gtgccaccgc tgccggaaag
      781 tcggcagaaa gtgccgcatc gtctgcttca acagccacaa cgaaggctgg cgaagccact
      841 gaacaggcca gcgcagcagc gagttctgct tccgcagcga agacatccga aacgaacgcg
      901 aaagcgtcgg aaaccagcgc agaatcctca aaaacggctg ccgcatcgtc agccagttcg
      961 gcggcgtcat cggcatcatc tgcgtctgct tcaaaagatg aggcgaccag acaagcgtca
     1021 gcagcgaaga gcagcgccac gacggcatcc acgaaggcga cagaggcagc tggcagtgcg
     1081 acggcggcag cacagagcaa aagtacggca gaatccgcag caacgcgcgc tgagacagcg
     1141 gcaaaacggg cagaggatat tgcatccgcc gtggcgcttg aggatgcgag cacgacgaaa
     1201 aagggaatag tacagctcag tagtgcgact aacagcactt ccgagtcact ggcggcaaca
     1261 ccaaaagccg ttaaggccgc gtatgacctg gctgacggga aatacaccgc acaggatgca
     1321 acgacagcac agaaagggat agtccagcta agcagtgtga ccaacagcac atctgaaacg
     1381 cttgcagcga caccaaaggc agtaaaaacc gcctatgaca atgctgagaa acgtctgcag
     1441 aaagatcaga acggtgcgga tatccctggc aaagacacct ttacgaaaaa tattggtgcc
     1501 tgccgtgcct tcggtgggtc agtaagcaca acaacaggaa actggacgac tgcacagttt
     1561 atcgagtggc tggattctca gggagcattt aaccatccat actggatgtg caagggttcc
     1621 tggtcttatg gcaataataa aatcattact gatactggct gcggtaatat tcatctcgcc
     1681 ggagctgtca ttgaagtaat ggggataaag tcagcgatga cgatccgcat taccactccg
     1741 accacatcca gcggtggcgg aacaaccaac gcccagttta cctatattaa tcacggaaca
     1801 gattattcac ctggctggcg aagggactat aactccagaa ataagcca
//
LOCUS       DAZPDU010000348         1825 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZPDU010000348 DAZPDU010000000
VERSION     DAZPDU010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.352"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            141..644
                     /locus_tag="ACHURS_004607"
     CDS             141..644
                     /locus_tag="ACHURS_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFR1240143.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(723..974)
                     /gene="yecJ"
                     /locus_tag="ACHURS_004608"
     CDS             complement(723..974)
                     /gene="yecJ"
                     /locus_tag="ACHURS_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFR1240144.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1089..1175)
                     /gene="azuC"
                     /locus_tag="ACHURS_004609"
     CDS             complement(1089..1175)
                     /gene="azuC"
                     /locus_tag="ACHURS_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFR1240145.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1438..1761
                     /locus_tag="ACHURS_004610"
     CDS             1438..1761
                     /locus_tag="ACHURS_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFR1240146.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
BASE COUNT          473 a          431 c          406 g          515 t
ORIGIN      
        1 tattcttatt tcagatcatc gtcagaattg actccacgat cacatttcgg accggcagaa
       61 aggaattatt ctgcaaacag taattatggt gttttgattt atcttgcacc tctccacttc
      121 tggatataag gatattaggt atggcaaccg ctggaatgct tctcaaactc aactctcaaa
      181 tgaaccgcga gttttacgca tccaatctct accttcacct gagtaactgg tgttctgaac
      241 agagtctgaa cggcaccgcc actttccttc gcgcccaggc acagagtaat gtgacccaaa
      301 tgatgcgcat gtttaacttt atgaagagtg tcggcgctac ccccatcgtt aaagccattg
      361 atgttcccgg tgaaaaactg aactctctgg aagaactgtt ccaaaaaacg atggaagaat
      421 acgagcaacg ttctagtacg ttggcacagt tagccgatga agcgaaagaa ctgaatgatg
      481 attcaaccgt caatttcctg cgcgatctgg aaaaagaaca gcagcatgat ggtctgttgc
      541 tgcaaaccat tcttgatgaa gtgcgcagtg cgaaacttgc gggtatgtgc cctgtgcaga
      601 ccgaccaaca tgttctgaat gtcgtgtcac accagctgca ttgatcatca tcggcgctaa
      661 tgcattgcgc cgatgaaggt tttgagaaac cgctgcctca tctgtttgaa gcagcggttt
      721 ttttaatggg attcaccctg tggggtaaac ttgagttcaa taagcgcgat ggctttttgg
      781 attgcccgca tggtgaccgg gtctgcggcg gcgggatggt tagtaaagtc gatattcttc
      841 agctgactgg acattttttc acgaacttca acgggcgcga ttacatcgag aacatccaga
      901 atttgtttga taaccaactg gcaagcaacc acatcagaaa ccagttcctg atcggcattc
      961 agcggctggg acatcgtaaa ctcctgatag cattttgaaa gccgttatag tagcggcttc
     1021 acatcttcag cgatagtcac atccaccgtc atcaggacac aaaaaaacct gccggagcag
     1081 gttttttgtt atcggaacat attgcctggc ggtacgtctt tgaacgtctt gcaatagtta
     1141 ttgaacatac ttttcaggat tttgcgcagt ttcatcgcgg cactccgacc atttgttata
     1201 caggtgttat tgtctttgcg cttataatat gacaaccatc acaaaaatca atctttatgt
     1261 gatacaaatc acataaatac ccctttaatg ttataaaaat gataatcaaa aaacagcccc
     1321 cctatttctg acacctacag atggcaagaa atagcgcctg ccaggcgtct tttccggcca
     1381 ttgtcgcagc gctgtaacgc gtaaaatagt gctttctctt actcttctgg ctggaccatg
     1441 agacttctga ttctgactct ttcattaata acgctggcgg ggtgtacggt tactcgccaa
     1501 gcccatgtga gcgaagttga cgcggcaaca ggtattgtac ggttggttta tgaccaggct
     1561 tttttgcagc atgcccatac cgatcgttat gtcagtcgcg ggatcgccga tcgcgcatgc
     1621 cagcaggaag gctataccca cgcggtcccc tttggtcagc cagtaggcaa ctgtagcctt
     1681 tttgcgggtt ccttatgttt gaatacggaa ttcactttgt catatcagtg ccatcattcc
     1741 gccttccctg tctttctcta aatattttaa tcagcgaggg gatcttcgct gattaaagaa
     1801 atagcggtaa tgcgttttat tccca
//
LOCUS       DAZPDU010000349         1743 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZPDU010000349 DAZPDU010000000
VERSION     DAZPDU010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.353"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..415)
                     /locus_tag="ACHURS_004611"
     CDS             complement(<1..415)
                     /locus_tag="ACHURS_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HFR1240147.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELE"
     gene            complement(431..1105)
                     /locus_tag="ACHURS_004612"
     CDS             complement(431..1105)
                     /locus_tag="ACHURS_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFR1240148.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWHLTIIDDHGN
                     YISDKI"
     gene            complement(1185..1727)
                     /locus_tag="ACHURS_004613"
     CDS             complement(1185..1727)
                     /locus_tag="ACHURS_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1240149.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
BASE COUNT          495 a          388 c          313 g          547 t
ORIGIN      
        1 tttccagttc ttccacccag gcctgcggga cactgaaatc gagacgaaaa accccgatat
       61 cccaggtata gctcccaccc tgaacaagtt gctcaaatgt taaacattgc ttaccgctgg
      121 cgaagttatc gctattaatg cctaaccgct tgataacttc tcttgataaa catgtttctt
      181 gcgggttgtc tttaacaata atctcatatt tcccgcgcca ttgcttattg acataaatat
      241 cgatgtcata ctgccccggt aagggttgat tatcatcaag acgaatattt gcaacctgct
      301 ggtctttcat tccacctatc ataaaatggg tatcaaaggt ttcttcagct gcataagctt
      361 caatgccgag caataacgct acgattgctg atgcaagtgg ggtcattctc aacataggca
      421 gctcctgcaa ttaaattttg tcactaatat agttgccatg gtcatcgata atggtcagat
      481 gccagttatt tgcattatta cttttgacat taacactctg actttctaag ggggcaatca
      541 taatagtttc ataattgact ttgacattat tagctttgac atccgaaatc gtcacccaat
      601 tagctgagtc attttttatc accaaaccat tgccactgcg atttaccagc aattttttaa
      661 atgtcgcttt atttaccgga gcaatacccg ctggtcggta aaacaactta attctgtttt
      721 gcatcgcaaa cttcagtgca ttcttacctt cttgctctgg actatttggt ggaatatcca
      781 gaacattcag ataaaaaatg ctttctttat tagtgggcag tttattcggc ataattttga
      841 tttttacttg ctgcccggaa tttgccccta tttttgccac tggtggcgtt aacatgaaag
      901 gaacctgaat tttttctggt ggtaatgacg tatcgccatc atcaatccac gcctgcagca
      961 gcgaagaacg gtttccctgg ttcatcaact gcaccatcac ttctttattt tctgccgggt
     1021 aaataatgcg cgtcccgtag ataacaattc cggcatgtgc aggaaggacc atagaaaaaa
     1081 tgagtaaaga taataatcct ttcattttaa tgcctcttaa attaaaaggc cgccatggga
     1141 tagcggccat gtttattacc tggagaaact tacaatttta tttattaatc ctgcgccaca
     1201 cgcataacca gagaagataa cacatcacct gattgcactg agttatctgt cagtttcagg
     1261 tattttgctt taaacggaat agcatattga ccttccgctg ttttataact ctcaatgtta
     1321 aaaggtgcag aaacatccag gacatcgctg ttatcaccta aatagagata gtaacccacg
     1381 gtatcgttgc tactggctat cgccttatcg ttaccggtta ttttatcgtt ggtaatcaac
     1441 gagagtttta caccagcaac ttctttcggg cagctaattt ttaacatgct gctggttaca
     1501 ccgactgcat catcataggt agaatattta gctttacctt caaagacagt ggttggtaca
     1561 gtcggcatat taatacttac tgaatcacca cccgcttcga atttacacgg agattctgca
     1621 atattcccct taatagttaa tgttccggta tcaacgtctg cagcaaatac tccagcgctc
     1681 ataaaaaaag aagaaaagac agcggcagca ataattgaac gtttcataat atgttcctta
     1741 ata
//
LOCUS       DAZPDU010000350         1723 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZPDU010000350 DAZPDU010000000
VERSION     DAZPDU010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.354"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..959)
                     /locus_tag="ACHURS_004614"
     CDS             complement(<1..959)
                     /locus_tag="ACHURS_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR1240150.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGPQI
                     YFSMSDGQQNTLHRPLADAVTAWFPENKQSDVSQIWHAFEHEE"
     gene            complement(1560..>1723)
                     /locus_tag="ACHURS_004615"
     CDS             complement(1560..>1723)
                     /locus_tag="ACHURS_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR1240151.1"
                     /translation="RISKRYDDVEVIVKATSNDGLSVTRTADKDSAKTFVQETLKDTW
                     ESADEWFVH"
BASE COUNT          448 a          294 c          422 g          559 t
ORIGIN      
        1 tgctcttcat gttcaaaagc atgccatatc tgtgatacat cagattgttt gttttccggg
       61 aaccatgctg tcacggcatc agccaggggg cgatggagtg tattctgttg tccgtcactc
      121 atggagaagt aaatctgcgg gccgtggtag tccggcgaag aggttaatct ttgcagggat
      181 tgcagagcat gggatgacaa tgggttatca ctaatatcta ttgaacattc attcctcaga
      241 ttaagaatgc tttccgggat atgacttatc tgatttatgt caaaataacc gttccttaaa
      301 ttttccggca ggtatggaat atcggttaac atattgtcga ttgcccatat ggagtgaatg
      361 ttgtgaggta gaggggggag ttcactaaga agattcccta ttgcgctaat ctcttgtaaa
      421 gaaaatggta aaggtggtaa aactgccagc ccattacatg atacatctaa tgtttccagt
      481 agttctggta aaacaggaag agaacataat tgattatttg agacatgaag ctccttcaaa
      541 gatatgggga gtgtgggtag tgtgattagt tgattgtggg acgcattcaa taatttaagt
      601 ccttgaggca aagcaggcag ttcaataagt ctgttatagc tgacattaag ctgtgtaagg
      661 gacgcaggca atggggagat taagcttaaa ttatttttac ttatattaat tgatttaatt
      721 cccgggggga tttcaggtaa tgttgtcagg cctaattcag acaagtccag gttcgtctct
      781 tggttttgta gacatgatac tagtcgctga aaagcgatgt ctcgttgttc ttcttgtatg
      841 cggttatttt tccattcagt ccaatgggtt agataatttt catatgcacg gctagtgtcg
      901 attgaataag tggagaacga atttgaaatt aatctgtgat tgttatttgt cgggagcata
      961 aatatcaggg cgtattcttt atgttgagag gtgttggatt cttttttatc tgctttgcaa
     1021 tgttattggt tcccttcagc agtagacagg aacttctgga ggaggcggat atatagtttg
     1081 tgtaagcata ttaactccat atgttatata ttgaagaact tctgctttac actatcctaa
     1141 actggatcgg tttaatgtaa aaaaacgata ctttttgggg gagggggcaa tttttcctta
     1201 aggtatgaaa aaaggagctg aagctatata atagctatta atgccactgg tatcagtgaa
     1261 tcatgtatat aaaaaactgt gttaaggagg ctgtccatta gcggtttttt attcttctgg
     1321 aaccgcggca tctttgcgta tctttttgtc ttctcaccgt ctggtcggtg ttttgctgag
     1381 actgctaatt tcctgtcttt gctagtgctg tactggtact gcccaatcat gattggcggg
     1441 ggcgggcgga gttgagaccg cagacacgtc gtatgcaaga acgtgctgcg gctggctggt
     1501 gaacttccga tagtgcgagt attgaatgat ttccagccgt tactgatttt acgtgctaat
     1561 tagtgaacaa accactcgtc agcagactcc caggtatctt tcagagtttc ctgaacaaaa
     1621 gttttagctg aatctttatc ggcggtgcgc gtaacagaaa ggccatcgtt gctggtggct
     1681 tttacgatca cctctacatc gtcataacgt ttactgatgc gtc
//
LOCUS       DAZPDU010000351         1720 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZPDU010000351 DAZPDU010000000
VERSION     DAZPDU010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.355"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..1138
                     /locus_tag="ACHURS_004616"
     CDS             <1..1138
                     /locus_tag="ACHURS_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR1240152.1"
                     /translation="TWYGWDGDRLTTIQTGTTRIQTVYQPGSFTPLLRIETENGEQAK
                     ARHRSLAEVLQEDTGVTLPAELSVMLGRLERELRAGAVSAESEAWLAQCGLTAEQMAA
                     QLEAEYIPERKLHLYHCDHRGLPLALISPEGETAWQGEYDEWGNLLGETSAQHLQQSL
                     RLPGQQYDEESGLYYNRNRYYDPLQGRYITQDPIGLRGEWNLYKYPLNPVRFIDSLGL
                     KFHVNGDPSDFNQAVEYLKQDSQMKETIDFLSSSEETINIEYIEGTNVRFNSNNMTIY
                     WNSRASLFCSTELNSKSQSPALGLGHEFAHAQYCLLDKENFMALLSRTDKKYENKEEA
                     RVITIIESRAAKTLGECTRGAHSGLPFYRVDGPLQTMKITGTPE"
     gene            1150..1599
                     /locus_tag="ACHURS_004617"
     CDS             1150..1599
                     /locus_tag="ACHURS_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-h1"
                     /protein_id="HFR1240153.1"
                     /translation="MMKYLMVLLSLFSGSVLGMGRVNELCGIDSVKTIEIINLPSYVT
                     TLVPLSKEGLNEIYRYKVVVNEIRDLYAGKIIDLLQMKYFRKEKYNNIRWGGSIISKG
                     NNKCEIYFDAFGECGSVNGINVCFEKNEMIGWIKKEIPLLSQKIGGL"
BASE COUNT          516 a          286 c          495 g          423 t
ORIGIN      
        1 gacatggtac gggtgggacg gtgacaggct gacgacgata cagaccggca ccacgcgtat
       61 ccagacggta taccagccgg ggagcttcac gccgctcctg agaatcgaaa cagagaatgg
      121 cgaacaggcg aaggcgcggc accggagcct ggcggaggtg ttgcaggagg acacgggtgt
      181 gacgctaccg gcggagctgt cggtgatgct ggggaggctg gagcgggaac tgcgcgcggg
      241 agcggtgagt gcggagagtg aggcatggct tgcgcagtgc gggctgacgg cggagcagat
      301 ggccgcgcag ctggaggcgg aatacatccc ggagaggaaa cttcatcttt accactgcga
      361 ccaccgggga ctgccgctgg cgctcatcag cccggaaggg gaaacggcgt ggcaggggga
      421 gtatgacgaa tggggaaacc tgctgggcga aaccagcgcg cagcacctcc agcagtcact
      481 ccgtctgccg gggcagcagt atgatgagga gtcggggctg tactacaacc gcaaccggta
      541 ctatgacccg ttgcagggaa ggtatatcac ccaggatccg atagggctga gaggtgaatg
      601 gaatctgtat aaatatccat taaaccctgt tcggtttata gattctttgg gtttaaaatt
      661 ccatgttaac ggtgatccaa gtgattttaa tcaggctgtt gagtatttaa aacaagattc
      721 acaaatgaaa gaaactatag attttttatc aagctctgaa gaaactataa atatagagta
      781 tattgaagga accaatgtta gatttaattc gaataatatg acgatatatt ggaatagtag
      841 agcatcttta ttttgttcaa ctgaattaaa ctcgaaatca caatcgcctg cattaggttt
      901 gggacatgag tttgctcatg cgcagtattg ccttcttgat aaggaaaatt tcatggctct
      961 tttaagccga acagataaga aatatgagaa taaagaagag gcaagggtta ttacaataat
     1021 tgaatcgcgt gcggcgaaaa ctttaggtga gtgtaccaga ggagctcata gtggactgcc
     1081 tttttatcgg gtggatggac cgttgcagac gatgaaaata acgggtacgc cagaatagtg
     1141 aggtttttta tgatgaaata tttgatggtt ttattgtctc tattttctgg ttcggtattg
     1201 gggatggggc gagtgaatga attgtgtgga atagatagcg tgaaaacgat agagataatt
     1261 aatcttccat cgtatgttac cacgttagtt ccgcttagca aggaaggtct taatgaaata
     1321 tacagatata aagtggtggt gaatgaaatt agagatcttt atgctggtaa gataattgat
     1381 ttattgcaaa tgaagtattt cagaaaagaa aaatataaca atataagatg ggggggtagt
     1441 atcatttcga aaggtaataa taaatgtgaa atttattttg atgcttttgg tgaatgtgga
     1501 agtgtgaatg gcattaacgt gtgttttgaa aaaaatgaaa tgataggttg gattaaaaaa
     1561 gaaatccctt tgctgtcgca aaaaataggt gggctttaaa tggtagggca atattcaaag
     1621 acagtatagg taactatttg tatggccatg taaggtaaaa gaaaaggaat aaaccatgag
     1681 cggaaaaccg gcggcgcggc agggcgacat gacccgcagg
//
LOCUS       DAZPDU010000352         1691 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZPDU010000352 DAZPDU010000000
VERSION     DAZPDU010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.356"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            132..386
                     /gene="bssS"
                     /locus_tag="ACHURS_004618"
     CDS             132..386
                     /gene="bssS"
                     /locus_tag="ACHURS_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415578.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFR1240154.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFQVNEYRRH"
     gene            501..1619
                     /gene="solA"
                     /locus_tag="ACHURS_004619"
     CDS             501..1619
                     /gene="solA"
                     /locus_tag="ACHURS_004619"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFR1240155.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPEQPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
BASE COUNT          439 a          418 c          415 g          419 t
ORIGIN      
        1 agaaatttat ctgttccccc ctttaaaaat tcggtgaata cccttactta ttggtatact
       61 gaagttgctt cagataaaaa cgcattgaac ctcgaataac gttgtctagt aacacgaatt
      121 agggggccat gatggaaaaa aataatgaag tcattcagac tcatccgctc gtagggtggg
      181 acatcagcac cgttgatagc tatgatgcgc tgatgttgcg tttgcactac cagaccccaa
      241 ataagtccga gcaggaaggg actgaagttg gtcaaacgct ctggttaacc actgatgttg
      301 ccagacaatt tatttcgata ttagaagcag gaatcgccaa aattgaatcc ggtgatttcc
      361 aggtaaacga gtatcggcgt cattaacatg acatgaccat ccacccaaaa ggcacctcac
      421 cggtgccttt accatttctg ggttgtatat ctgcattcag ttaattactc tgcttaaacc
      481 atgagagcag agagaaaaaa atgaaatacg atctcatcat tattggcagc ggttccgtag
      541 gcgctgccgc cgggtattat gcaacccgcg ccggtttaaa cgtgctaatg accgacgccc
      601 atatgccacc gcatcaacac ggcagccacc acggcgatac gcgattaatt cgccatgctt
      661 atggtgaagg cgaaaagtat gtcccgctgg tcctccgcgc gcaaacgctg tgggatgaac
      721 tttcccgcca caacgaagat gatcccattt ttgtacgctc tggtgtcatt aacctcggcc
      781 cggctgactc cgcatttctc gccaacgtcg cccacagcgc cgaacagtgg caactcaacg
      841 ttgaaaagct cgatgcgcaa gggattatgg cccgctggcc agaaatacgc gtcccggaca
      901 actacatcgg cttatttgag actgattccg gttttttgcg cagcgaactg gcgattaaaa
      961 cctggatcca actggcgaag gaagcgggct gtgcgcaact gttcaactgc ccggtcaccg
     1021 caattcgtca tgacgatgat ggcgtaacta ttgaaacggt tgacggtgag tatcaggcga
     1081 aaaaagcgat tgtctgcgcg ggaacatggg taaaagacct gctcccggag cagcctgtcc
     1141 agcccgtacg taaagtattt gcctggtatc aggccgatgg ccgctatagc gtgaagaata
     1201 aattcccggc gtttaccggt gaactgccca atggcgatca atattatggt tttccggcag
     1261 aaaacgacgc gttgaagatt ggcaaacata acggaggcca ggttatccat tcagcggatg
     1321 aacgtgttcc gtttgcggaa gtggtcagcg atggttcgga agccttcccg ttcttgcgca
     1381 atgtattgcc gggtatcggt tgctgcctgt acggcgctgc ctgcacctat gataattcgc
     1441 ctgacgaaga ttttattatc gataccctac ccggccacga taatacactg ctcattaccg
     1501 gcctgagtgg gcacggtttt aaatttgcgt cagttttagg ggaaatagct gccgattttg
     1561 cgcaagacaa aaaaagcgat tttgatttga cgccattcag gctttcccgc ttccaataat
     1621 catcattacg gcctccagaa tgaggccgtt tttacaggag tatttaatgc gtcggctgtt
     1681 gcactatctc a
//
LOCUS       DAZPDU010000353         1679 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZPDU010000353 DAZPDU010000000
VERSION     DAZPDU010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.357"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            279..1322
                     /locus_tag="ACHURS_004620"
     CDS             279..1322
                     /locus_tag="ACHURS_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR1240156.1"
                     /translation="MNDRLCFEVHDNKGYFVFSDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNASLKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
BASE COUNT          487 a          366 c          351 g          475 t
ORIGIN      
        1 aattgacatt tttaaattgg caattacaaa atacagcctt gcagttattt cgataccttt
       61 agcaaaggaa aagttgattc agatataatt tagcatttca atcatctgac atcattataa
      121 aaaatgagat tcacataaag ataaaattat tgttctcaag gaaagcatga aggggcttcg
      181 taagcaatat tcattcggtg ccaagacagg acttgataca cagaatctca acttttatgc
      241 tctcagacta tatcaccaca tgttttacag gctcagaaat gaacgataga ttatgctttg
      301 aagtgcacga taacaagggt tatttcgtat tttcggatac ctggtttggc ccattgctgg
      361 gggagtttga agaagtgctt gatgcatacg acgctgatga aatcagcgag acgagttaca
      421 tcaacaaact ccgacgcctt gctcaacggg agccggactt tattgatata cacgctcatc
      481 tggcatatgc atttctggaa cagaatgccc cgcgtaaggc actgaacgcc tctcttaaag
      541 gactggcggc aggtaatcgt atcatcccgg aaagtttctg tggtgaaata atctggatgc
      601 atcccgaaaa ccggccttat ctgcgcgcgc tttatgcagc aattctcgca aatgtgcact
      661 tacagcgcca tcaggatgcg gttatgctca ctgataaaat actggcatac aatcctgaag
      721 ataatcaggg ggctcgctgg ttgttgggtt cagagctgtt acgcaccggc gatcatgagc
      781 gagccttcag tgtactgaaa gagcatgctg atgaattttc accttactgg tatgaactcg
      841 gtctcctgca ttttctgaac ggggaacatg ttaaggccgc cacagctttt cgccatggct
      901 ttgccacaaa cacttacatc gcagaaatgc tgtgcggaaa tctgcatcca tttccacttg
      961 ctgtgtggca tgacttctcc ggctctctgg atacggcaga agattactat gcaacatact
     1021 caccattatg gggacaatat ccggaagcat tgctgtttgt aaactggctc tataaccatt
     1081 cctccgtgct gcatgaacga tcagaaatca ttaaatgcgc cgaaatgctg attcaggaag
     1141 atgatttcga aatatgcgaa agtattttaa gacagcagga gcacctgtgg aaaagaattg
     1201 acaagacact ttctgaggaa attgtacaga aatgcagaaa tatgaacggt gaatatatct
     1261 ggccctggat attgcccttt tccgcagcag ggatgaaaca ttcaagcatc cagtatcagt
     1321 agatattgca ttagtgtaac tttaaaacaa gtaataattt attatccaat aataacactg
     1381 tgttccttcc ccaggtgatt accctggggt tattttctga aagtttcctt tgcaaaagga
     1441 tagcgttgta aattacttta acgaattact ccatgaactc attaaagttc tttcgtaata
     1501 ctttatcgtc aaacacgtta tagctggcat caaaaaccac agatttaccc tgccagcggg
     1561 taatcccctc ctctggcgtt ttcggttgaa ctggtatccc cgtggatttg cccctatatt
     1621 tccagacatc tgttatcact taacccatta caagcccgct gccgcagata ttcccgtgg
//
LOCUS       DAZPDU010000354         1676 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZPDU010000354 DAZPDU010000000
VERSION     DAZPDU010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.358"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..348)
                     /locus_tag="ACHURS_004621"
     CDS             complement(<1..348)
                     /locus_tag="ACHURS_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFR1240157.1"
                     /translation="MLNQETAKAARTDSGYILRAPRRMRVADAVAQYMRVPMGAGNSV
                     PWDPLVAPYVIEPMNCLASREYDAVIFVGPARTGKTIGLIDGWVIYNVICDPADMLII
                     QMTEEKAREHSKKR"
     gene            complement(348..842)
                     /locus_tag="ACHURS_004622"
     CDS             complement(348..842)
                     /locus_tag="ACHURS_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFR1240158.1"
                     /translation="MDRELKNLTLNISQLAALSGVHRQTAAARLQNLPVAGGHESNLK
                     LYRVVDIVSAFLALPPPVAEGEMDAHERKAWYQSERERLKFEQETAQLIPASDVRREF
                     AIWAKAVVQVLETLPDILERDCGLQPAAVSRVQSIIDDLRDQIALRVTEAGADDEEEL
                     QQEE"
     gene            1200..1376
                     /locus_tag="ACHURS_004623"
     CDS             1200..1376
                     /locus_tag="ACHURS_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240159.1"
                     /translation="MRDKPANHKRGKNPVCIVFDYSRTLAQKEFPVGLRSWLIREYGD
                     DTAHDVSGLNTFIR"
     gene            complement(1518..1670)
                     /locus_tag="ACHURS_004624"
     CDS             complement(1518..1670)
                     /locus_tag="ACHURS_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240160.1"
                     /translation="MPSMIAIILLIILHIWLCRQGGAHFWSESRLNISLLMLDIEHFA
                     RGKGLR"
BASE COUNT          434 a          465 c          382 g          395 t
ORIGIN      
        1 tcgttttttg gagtgttcgc gggctttttc ctccgtcatc tgaataatca gcatatcagc
       61 aggatcgcaa atcacgttgt aaatcaccca gccgtcaatc aggccgatag tcttgccggt
      121 tcgtgccggg ccaacaaata tcactgcgtc gtattcacgc gaggccaggc agttcatcgg
      181 ctcaataaca tacggtgcca ccagcggatc ccacgggact gagttccctg cccccatggg
      241 cacccgcata tactgagcaa cggcatcagc aacccgcatt cgtctcggtg cgcgaaggat
      301 ataacctgaa tcggttcgtg ctgcctttgc ggtttcctga ttcagcatta ctcctcctgc
      361 tgtaattcct cctcatcatc cgcacctgct tcagtcaccc gcagggctat ctgatcgcgc
      421 agatcatcaa taatggactg aacacggctc acagcggcag gctgcagacc gcagtcacgt
      481 tcaagaatat ccggtaatgt ctccagcacc tgcacgaccg cttttgccca gatggcaaac
      541 tcccgtctga catcactggc cggaatgagt tgtgccgttt cctgttcgaa cttaagacgc
      601 tcacgttcag actgatacca ggctttgcgc tcatgcgcgt ccatttcgcc ttctgcaacc
      661 ggcggtggta atgccagaaa tgccgacaca atatcaacca cccgataaag cttgaggttg
      721 ctttcatgcc cccctgcaac gggtagattt tgcagccttg ccgcagcagt ctggcgatgt
      781 acacctgaca gtgccgccag ttgactgata ttcagcgtca gattttttaa ctctcgatcc
      841 atacccgctc cagaatgttt taaacatgca tcttgcgaac aactttaggc aaacggtgtt
      901 agtgatgaac aaaaaacaat caaaatcgac accataaaaa taaaaccact gtaatatcaa
      961 tccattacag tagtggtgat gacgaatgaa atttcaaaaa ctagcctttt tccgcgccgc
     1021 tcccgccccg tggcaggcca ccccaccggg aggacccgtc agcctgacag ccatgacgaa
     1081 cgtctgatac agcgccctgc atgaatgaca tcgggataat ccagaaagga atagcatcgt
     1141 acccacaaga atcagtgtaa gtgtcctgtt tcttccaccc ccgcacagga ctggcgagca
     1201 tgagggacaa acccgcgaac cataaacgcg gtaaaaaccc ggtgtgcatc gtttttgatt
     1261 attcccgcac actcgcgcag aaggagttcc ccgtcgggct acggtcatgg ttaatacggg
     1321 aatacggcga cgatacagcg catgatgtgt caggcttgaa tacctttatc cgttaaaagg
     1381 gatatcagtt aagttatccc gtgtagggta taagccatta tcaaagccac tctgtaggaa
     1441 gtggcttttg taatggcaat aaaaagcccc gcgaatgcga ggctaaatcc tggtatttgt
     1501 aatgactggc tcttatctca acgcagcccc ttaccgcgcg caaaatgctc aatatcaagc
     1561 atcagcaatg agatgtttaa tctggattca ctccagaagt gagcaccacc ctgtctacag
     1621 agccagatgt gaaggatgat gagtaaaatt atcgctatca tcgaaggcat tgcgtc
//
LOCUS       DAZPDU010000355         1638 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZPDU010000355 DAZPDU010000000
VERSION     DAZPDU010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.359"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(8..118)
                     /locus_tag="ACHURS_004625"
     CDS             complement(8..118)
                     /locus_tag="ACHURS_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240161.1"
                     /translation="MGIKVQRLSLWMINTVLLLLPINNHQANTIKLIFEM"
     gene            complement(171..575)
                     /locus_tag="ACHURS_004626"
     CDS             complement(171..575)
                     /locus_tag="ACHURS_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFR1240162.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHIYGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            complement(796..1527)
                     /gene="bluR"
                     /locus_tag="ACHURS_004627"
     CDS             complement(796..1527)
                     /gene="bluR"
                     /locus_tag="ACHURS_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFR1240163.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWNRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
BASE COUNT          446 a          352 c          344 g          496 t
ORIGIN      
        1 caagtgatca catttcaaat atcaatttga tagtattggc ctggtgatta tttatgggta
       61 gcaataaaag gacagtattt atcatccata gggatagtct ctgtactttt attcccatta
      121 tgctaatgcc ttactgaatt atgaagcatt tcttaagcat ccaactttag ctagattaat
      181 ggtttattat tttctacatc ttcaatatat aaaagcgtat tatcaatggc gtagtaactg
      241 cgtttgttat gattaacatc agtaacccaa cggaaaacgc ccgcgcctgc cagtgttgaa
      301 cagtattccc gaaatgtaga ttttccgtaa atatgaagca atgcggcctc ttttatttta
      361 gcagggttct tggtcgtact aacttttaac aggttcctgg ttcctcttaa taacaaaacc
      421 gtgtcatcgt gagtaataat tctgatgtta tccgtagcca gataataaat gtaatgtgca
      481 atacggtgat gttttaattc tgaataaaac caggagaagt tttgctcttt tctcacttgc
      541 tcaaacatct tttgaaaaac aacgacctga tccatcagga taataacctc ttgttagttg
      601 tgagactgcg tagtgtgcac gatcggtttt accacttcaa tctggtctgt cctttggctg
      661 tgatatgtac agagtgtgat agagggatta cctgaattct tccggtgagc attttgcaac
      721 ggaccagctc cggtacaaat gctgttgtgg gttcagatta taacattctg tctaaggggc
      781 gggataaagg tgaaattagg gggcatgaaa gatgacttta taaccttgct caccccaatg
      841 ttgtaaaagt tcgttttgcc ttctcgttgg tgccatgcct gtccagacaa tcaatgtttg
      901 cgtcgggaac agttccgggc gcggcgattc aatgggatca gcaagaacag aaatgtgcca
      961 tcctgacaat gataaacgcc atgcttccag ccacaatcgt gctctctctt caacattcca
     1021 cgccatcaga atggcttctt taccgggctt acggcgcatt tcgaaaagtg aggctgctgc
     1081 atattcaatt aatgcgccgt caaacatact gctcataatg cgggaggtgt tgtgatcaag
     1141 cacgagacgc tggcgaacag gaaggtaaac atgattaatc aattgatcta ctgggtactc
     1201 tcgacccagt gaaataattc tcgcgcgtag tttggcagga ttagccatac gaagaattga
     1261 catcatctct tcttgcaggc ggttccagtc atcttccgta tcctggctgg tggtttccag
     1321 taatgcttta actttgccta cagggacgcc attacttatc caacgcttga tctcttcgat
     1381 gcgttgtatg tcttcttcat caaagagtcg gtgtccgcct tcactgcgct gtggttttaa
     1441 caaaccgtag cggcgttgcc aggcccggag agtgacagga ttaatcccgc aacgttcagc
     1501 aacatcacca atgctgtaat aagccacaat tcctccttgc ggtcataaat ctccgccgcc
     1561 tgtacacgac ccaataatac tttgtacaat atacgctaaa attgtacaaa gtataaataa
     1621 gatttcagct aaattgga
//
LOCUS       DAZPDU010000356         1629 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZPDU010000356 DAZPDU010000000
VERSION     DAZPDU010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.360"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..93
                     /locus_tag="ACHURS_004628"
     CDS             <1..93
                     /locus_tag="ACHURS_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1240164.1"
                     /translation="RFYLEEQGWTLVNRGAIKNMTVEQLLKIHC"
     gene            104..292
                     /gene="dicB"
                     /locus_tag="ACHURS_004629"
     CDS             104..292
                     /gene="dicB"
                     /locus_tag="ACHURS_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFR1240165.1"
                     /translation="METLLPNVNTTEGCFEIGVTISNTVFTEDAINKRKHERESLNKI
                     CIVSMLARLRLMQKGCWR"
     gene            289..480
                     /locus_tag="ACHURS_004630"
     CDS             289..480
                     /locus_tag="ACHURS_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFR1240166.1"
                     /translation="MNTAFALVLTVILNTGEPAELVTGVYNSMKECVAAAAEQKIPGN
                     CYPVDKIIHQDNKEVPAGL"
     gene            574..>1629
                     /locus_tag="ACHURS_004631"
     CDS             574..>1629
                     /locus_tag="ACHURS_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFR1240167.1"
                     /translation="MSKVFICAAIPDEKATKEEGAKPVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESEPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMCPEIKLHAIGWIKHKCKPGAKWPEIQAEMRIWKKCREGERKETGKYTSVVDLARA
                     RANQQHTENSTGKISPAIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KNEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYITEY
                     LTTKGVFEDEGTNQSATD"
BASE COUNT          498 a          361 c          391 g          379 t
ORIGIN      
        1 cggttttatc tggaagaaca gggatggact cttgttaacc ggggcgcaat taaaaatatg
       61 actgtagagc aattactcaa aattcattgt tgaggacgcg ataatggaaa cgttattacc
      121 aaacgttaat acgactgaag gttgttttga aattggtgtc actatcagta acacggtatt
      181 tactgaagat gccattaaca agagaaaaca cgaacgggag tcattaaata aaatatgcat
      241 tgtttcaatg ctggctcgtt tgcgtctgat gcaaaaagga tgctggcgat gaatacagca
      301 tttgcactcg ttctgaccgt tattcttaat acgggcgaac cagctgaact ggttaccgga
      361 gtatacaact ccatgaaaga atgcgtggct gctgcagctg aacagaaaat tcccggtaac
      421 tgttatccgg ttgataaaat cattcaccag gataataaag aagtcccggc aggtctttaa
      481 agcagccacg taataaatat ccggtttcat ttttatatgc cagcaatggc agggatttgt
      541 tcacccttaa atctgtaatg aggttaaaac aaaatgagta aagtctttat ttgcgccgcc
      601 attccggacg aaaaggcaac aaaagaagaa ggcgctaaac ctgtagccac tgccattgaa
      661 gccggcgacg aacgccgcgc ccgcgcaaaa tttcactggc aattcctgga acattatccg
      721 gctgctcagg actgcgctta taaatttatt gtctgcgagg ataaacctgg tataccccgc
      781 cctgccctcg attcctggga tgctgaatat atgcaggaaa accgctggga tgaggagtct
      841 gcttcctttg tcccggttga gactgaatcc gaaccgatga acgtcacttt tgacaagctg
      901 gcccctgaag tacagaacgc tgtcatggtt aagttcgaca catgtgaaaa catcaccgtt
      961 gatatggtga ttagcgcgca ggaactgttg caggaagaca tggcaacatt cgacggacat
     1021 atcgttgaag cgttgatgaa aatgccagaa gttaacgcca tgtgtccgga gattaaactg
     1081 catgccatcg ggtggattaa gcataaatgt aagcctggtg caaaatggcc agaaattcag
     1141 gcagagatgc gcatctggaa aaaatgtcgc gaaggtgaac gcaaggaaac cggaaaatac
     1201 acgtctgttg ttgatctcgc ccgtgccaga gccaatcaac agcacactga aaattcaaca
     1261 ggaaaaatca gcccggccat tgctgccatt catcgcgaat acaagcagac atggaaaaca
     1321 ctggatgacg aactggccta cgctctctgg cctggtgatg tggatgccgg aaacattgac
     1381 ggcagcatcc atcgctgggc aaaaaatgaa gttatcgaca acgaccgcga agactggaag
     1441 cgtatctcgg catcaatgcg caaacagcct gatgcccttc gctacgaccg ccagactatt
     1501 tttggccttg tccgtgaacg tccgatcgac attcacaaag accctgtggc actgaacaaa
     1561 tacattactg aatacctgac tacaaagggc gtgtttgaag atgaaggaac aaatcagagc
     1621 gcaactgat
//
LOCUS       DAZPDU010000357         1625 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZPDU010000357 DAZPDU010000000
VERSION     DAZPDU010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.361"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            1..1436
                     /locus_tag="ACHURS_004632"
                     /pseudo
     CDS             1..1436
                     /locus_tag="ACHURS_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102217.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
BASE COUNT          460 a          419 c          394 g          352 t
ORIGIN      
        1 acgaagcaaa aaatgacaaa gtaagaaaac gcctcggtat taaaggcggt ttttactggg
       61 ctgaagcgaa aaaattatcc attgccatct cccgtggtgc tgttgcgatt gacgatgctg
      121 gctacgatga agatgacttt aaaaaacctg ttcgcgtcaa tttgcccgtt gttggtgacc
      181 tcccaccaga aggcgtgttt gatacggaat tctgcaaccg ttacgaaaaa ggcggggaag
      241 atggcatcac aatggtattt atcgcatctt ctccctctgt tcaggacaaa ccagccagca
      301 ctgacaatac caacgtcaac ggcgaagaca tgacagaaat tgaggagaac atgctcctgc
      361 cagtttccgg tcaggaactg cccattcgct ggcttgctca acacggcagc gaaaaaccgg
      421 taacgcacgt ttcacgcgac ggactccagg cattacacat tgctcgggct gaagaactac
      481 cggctgttac tgccctggct gtttcccaca aaaccagcct gctcgacccg ctggaaattc
      541 gcgatctcca caaactggtt cgtgacactg acaaagtttt ccctaatcct ggtaattcaa
      601 gcctgggact gataactgct tttttcgaag catacctgaa cgctgactac accgatcgag
      661 gactgctgac aaaagagtgg atgaagggta atcgtgtttc acacatcact cgcacggctt
      721 ccggtgctaa tgctggcggc ggaaacctca ccgatcgcgg cgaaggtttc gtacacgatc
      781 tgacgtcact ggcgcgcgac gtagccactg gcgtactggc ccgttcaatg gatctggaca
      841 tctataacct tcatccggca cacgctaaac gcattgagga aattatcgct gaaaataaac
      901 cgcccttttc tgttttccgc gacaaattca tcaccatgcc tggcgggctg gattattccc
      961 gcgccatcgt ggttgcgtcc gtaaaagaag caccaattgg gatcgaggtc atccccgcgc
     1021 acgtcactga atatctgaac aaggtactga ctgaaaccga tcatgccaac cctgatccgg
     1081 aaatcgtgga tattgcctgc ggtcgctcct ctgccccgat gccgcagcgt gtaacagaag
     1141 aaggaaaaca ggatgatgaa gaaaaaccgc aaccatcttg cgcaatggca gatgaacagg
     1201 caacggctga aacagtggaa ccggatgcaa ctgaacatca tcaggacacg cagccgctgg
     1261 atgctcagtc acaggtaaat gctgttgatg cgaaatatca gaaactgcgg acagaactcc
     1321 atgaagcccg gaaaaacatt ccgcccaaaa atcctgtcga tgcagacaaa ttactggctg
     1381 cctctcgcgg agaatttgtt gaagggatta gcgacccgaa tgattcgaaa tggattcccg
     1441 gtcatcatat ctcctcaaat gaagtcaaaa aaacggaaaa taaagtgcga cagactgaag
     1501 ataaacagca ccagaacagc gaaccggagg agcgaactga ggtgtactgg caatagcgga
     1561 cactaccatt tgttcttttt ttaagcagcc atctgatgat atttttccct gaaggctgcc
     1621 gggga
//
LOCUS       DAZPDU010000358         1615 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZPDU010000358 DAZPDU010000000
VERSION     DAZPDU010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.362"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            1..1615
                     /locus_tag="ACHURS_004633"
                     /pseudo
     CDS             1..1615
                     /locus_tag="ACHURS_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-mannosidase"
BASE COUNT          501 a          314 c          333 g          467 t
ORIGIN      
        1 ataatattat caaactgtcg aataaaatag gtcagcgtgt ttatactgtt gtctctgaaa
       61 tgtcgattga agcatggata acaaaagaac cgcagccttt tgtttatcgc cgtagcggcg
      121 cttaccagaa actcaccatt ggctcagact ggggtcaact gtttgactgt gcctggatgc
      181 gagtttatgg taaacgtcca gcagataaat ttagacacaa actcgtcgct ctcgttgata
      241 tcggtggcga aggtcttatt gtggataaga atggttcacc catctgcggc atcacgaata
      301 aagcatcatc ttatggcgta ccgccagata aaccaggtaa atgggtggtt gacctttctc
      361 tcgttagtga aaatgacgag gttgagtttt ggattgatgc tgcctgcaac gatttatttg
      421 gctatgttac taatggaggg attattactg atgttcatat cgcgacacgt aatcaactac
      481 tgaaatccct ttattatgat attgaggtgc ttttcgactg gattaatgat ggtcagaagt
      541 ttgaatccat acatcctaaa ggtatcaccc cagaacaatt tattgccaaa agaagtgagc
      601 gtactgacga aatcataagt actctcgaat atattgataa tacgttaatt acctttagca
      661 atgaagaaat aattaaatgt caaattgcaa tccaatccat aatcaataaa aacaataatc
      721 cttcagagtt caggattatg gctactggtc atgcccattt agatatagcg tggatgtggc
      781 ctttaagaga aggacgcaga aaagcaataa gaacatttgc caccgccctt gcaaacattg
      841 aaaaatatcc tgattacatt tttggcgcta gtcagtacca actttttcac tggataaaaa
      901 aagattatcc ctatttcttc gaaaagttaa aaaaacaaat cgccgcaggc cgctttgagt
      961 tacaaggttg cttctgggtc gaatgcgatt taaatctggt aagtggtgaa tcgctgataa
     1021 ggcaaatcat gcatgggaca cgattcacac tgcagaattt ttcaaaaaag ataaattatg
     1081 tctggcaacc ggatgtcttt ggattccccg caacgcttcc acaaattctc aaaaaatcag
     1141 gcattaacta tattgcatcg caaaagctct cacaaaacaa gattaataaa tttaataatt
     1201 accttttccg ctggcagggc ctggatggct cagaaattct gatgcataac tttcctgaag
     1261 acacttatga tagccgagca cgtgctcgct ctttagaata tattgaacaa aactataatg
     1321 aaaaagagat ctgtccctat gcgcttatgg tgtatggtgt atggtgtcgg tgatggcggc
     1381 gctggccccg gtgaggaaca tatagaacga ttaacacgta tacgcaatat tgatggctta
     1441 cctcatgttg atttcagccg tgttgataag ttcttcactt acgccgacgc ctttcgcgaa
     1501 agcttgccca ttatttcagg tgaactttat ttcgaggctc atcaagggtg ctttacatca
     1561 gagtcagcaa ctaaagctca taatcgaaat atggaaaata aattacatga tgctg
//
LOCUS       DAZPDU010000359         1612 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZPDU010000359 DAZPDU010000000
VERSION     DAZPDU010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.363"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..1062
                     /locus_tag="ACHURS_004634"
     CDS             <1..1062
                     /locus_tag="ACHURS_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFR1240168.1"
                     /translation="LAANVAHAAEVYNKDGNKLDLYGKVTALRYFTDDKRDDGDKTYA
                     RLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAGLKISEFGSLDYGR
                     NYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDFFGLIEGLNFAAQY
                     QGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKSDRTNAQERAAANP
                     LNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNKAQSFEVVAQYQFD
                     FGLRPSLAYLKSKGRDLGRYGDQDMIEYIDVGATYFFNKNMSTYVDYKINLIDESDFT
                     RAVDIRTDNIVATGITYQF"
     gene            1226..>1612
                     /locus_tag="ACHURS_004635"
     CDS             1226..>1612
                     /locus_tag="ACHURS_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR1240169.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKESKLE"
BASE COUNT          482 a          340 c          335 g          455 t
ORIGIN      
        1 ttagctgcga acgtagcaca cgctgccgaa gtctataaca aggatggtaa taaactcgac
       61 ctttatggca aggttaccgc tctacgttat tttactgatg ataagcgtga cgatggtgat
      121 aaaacttatg cccgtctcgg ctttaaagga gaaacgcaaa tcaatgatca aatgattggt
      181 tttggtcact gggaatatga ttttaaaggc tataacgatg aagccaacgg ctcgcgcggc
      241 aacaagaccc gtctggccta tgctggttta aaaattagtg aatttggctc tctggactat
      301 ggtcgtaact acggtgtcgg ctacgacatt ggttcatgga ccgatatgtt gccagaattt
      361 ggtggcgata cctggagtca gaaagatgtc ttcatgacat accgtaccac cggtctagca
      421 acctatcgca actacgattt ctttggctta attgaagggc tgaactttgc cgcgcaatat
      481 caaggcaaaa atgaacgtac tgacaacagt catctttatg gtgctgacta cacgcgtgcc
      541 aacggtgacg gtttcggtat ctcctcaact tatgtttatg atggctttgg tatcggtgcg
      601 gtatatacca aatccgatcg gacaaatgcg caggaaagag ccgctgctaa tcctctcaat
      661 gcctccggta agaatgcaga actatgggcc acaggtataa agtatgatgc caacaacatc
      721 tactttgcag ctaattacgc tgaaacatta aacatgacca cctatgggga tggttatatc
      781 tctaacaaag cacaaagttt tgaagtggtg gcgcaatatc aattcgactt cggcttgcgc
      841 ccatcactcg cttacctgaa atcgaaaggc agagatctgg gccgctacgg cgaccaggac
      901 atgattgagt atatcgacgt tggtgcgacg tatttcttca acaaaaatat gtcgacctat
      961 gttgattata aaatcaacct gattgatgaa agcgacttta cccgtgccgt agatattcgg
     1021 accgataaca tcgtcgctac gggcattacc tatcagttct aacgtcctct cctgaccagc
     1081 ccttctctgg gctggttttt gatttaagtg ctgctgtatg actccttaag taaaaatgag
     1141 caatttttga tatttctggt cattcacctc aattaatatt attccatatc ctccggggac
     1201 atcttactat aatatttatt gatgagtgat tatgaccaat attaatacag cttgtgtaaa
     1261 aaataatgcc agttatcaac ttaataacgc attacctaac aaagaaacca tctccagcaa
     1321 tttttgtgaa cgactagaac aatggggtaa taaatcggtt aataatggtg aagaaagagc
     1381 aattgccgtg gagcgaatta aagaagctta caattcgaac atggcatctc ttgatttgtc
     1441 atatcttgat ttgagtgaat taccgcctat cccctcgaca gttaatacgt taaatctgga
     1501 aaacaattgt ctaacttgtc ttgacttagc tgacaatgcc agtctcgtta atatcaacct
     1561 cagcgttaat aaaattaaca cgataacctt tccaaaagaa tcaaaactgg aa
//
LOCUS       DAZPDU010000360         1588 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZPDU010000360 DAZPDU010000000
VERSION     DAZPDU010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.364"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..987)
                     /locus_tag="ACHURS_004636"
     CDS             complement(<1..987)
                     /locus_tag="ACHURS_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFR1240170.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVRIYKVSSAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDGDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLHLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQYQVKYQHAKKNGDTFNAFIYFIK"
     gene            complement(1013..>1588)
                     /gene="rfaY"
                     /locus_tag="ACHURS_004637"
     CDS             complement(1013..>1588)
                     /gene="rfaY"
                     /locus_tag="ACHURS_004637"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HFR1240171.1"
                     /translation="RNINDTKVSLIDTKYGRYVFKVFAPKTKRNERFLKSFVKGDYYQ
                     NLIVETDRVRSAGLTFPNDFYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPEDVK
                     AEIKASMEKLHALNMLSGDPHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHL
                     GIANEIKDYGYYSVIYRTKLRKFIKKLKGKA"
BASE COUNT          497 a          330 c          271 g          490 t
ORIGIN      
        1 tttaataaag taaataaaag cgttaaacgt atcaccattt ttctttgcat gttgatattt
       61 cacttgatac tgcttttcat tcgtggctgg aataaacgcc acatcattcc agcaagaagc
      121 ctgataagcc tcattaaaat acttctggca gggataattc gtccagtcgt tccagggctt
      181 cgttacgcca acataatgaa tcagcttagt atcatcggta atggtctttt tataatcttc
      241 atcgctttta ttttttaact cataatcgat gccataaaag caatcataat cgccgctaat
      301 aaaaatacaa tgaccaaaga acagaatatt taaaatatcc tgatcaaggc atttatactt
      361 cgtgtgctgc gggtcacttg ccagcatagc aatggcttta ttcataaaat catttttcgc
      421 ccatgcttca agatgcagat atactacacc ggcgttgaaa taattccctg gaagtccctc
      481 tatagccaga cgcttcgctg gtttttcctg cataaaaggc acatctttaa taacagctgc
      541 aaacttatcc ccatcaaaat taatatcaag caatccagct aatgagcctt tacagatgac
      601 atcagcatcg atatataaca gattttcaat agtttcactc agatactgga acgcaactaa
      661 acggaagaat gttgcataag accactgttt caggctgggg aagatatcaa taaaggcact
      721 ggagacttta taaattctta cttcaacgtt agcatttttg ctggtaaaag cattaacacg
      781 ttgtagataa tcttcatcaa tgtagtcggt aaagagatga aacttaaccg caaggtccgg
      841 attatgcata acaactgatt gcatagatac tgccgcacca aataaaaagt tacggtcaat
      901 accgtaagcg atatttaaca cacgatcggt gttagcaatt tccccagaga cagcgacttc
      961 agtaatactc tcagctaata aatccatgtt ggttccgttt tgactgtgtg gtttacgctt
     1021 tgccttttaa ttttttaata aatttgcgta gtttggttct gtagattact gaatagtagc
     1081 cataatcttt tatttcgttg gcaataccca ggtgacgttc catggccaga cgatcacgag
     1141 ctttacgctc tgccgtacag cttttaccgg ataggtcaat tatcctgaca ccatctttac
     1201 taacgataaa gttaccccgg tgtgggtcgc ccgatagcat atttaaggca tgcaattttt
     1261 ccattgaagc cttaatttct gctttaacat cttctggaat gatcggcata tcattgagct
     1321 ctacgccctc gacatactcg ataagcatga taaaaacact ggcatagttg aaaattttac
     1381 gctcagccag aaaataaaaa tcattaggaa aagtaagacc tgcacttctg acacgatcgg
     1441 tttcaacgat caaattctga tagtagtcac cttttacaaa agattttaaa aaacgttcat
     1501 tccttttcgt tttcggggca aagactttaa aaacatagcg accatatttc gtatcaatca
     1561 atgatacttt ggtatcatta atattacg
//
LOCUS       DAZPDU010000361         1566 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZPDU010000361 DAZPDU010000000
VERSION     DAZPDU010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.365"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            207..>1566
                     /gene="istA"
                     /locus_tag="ACHURS_004638"
     CDS             207..>1566
                     /gene="istA"
                     /locus_tag="ACHURS_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISSso4 family transposase"
                     /protein_id="HFR1240172.1"
                     /translation="METCFKILQLKFDKKLTNRCIGLTLHISASTVFEVLARFKASSL
                     SWPLPADISHDTLEKLIFPPKDTSASELVMPDMLYFDTEMRKPGVTRQLLWMEYKAQA
                     GDKAMGYSHFCRCYRKWKKTRRLSMRQEHRAGEKLFIDFCGPTVPVINPDTGEIRRVA
                     IFVAVMGASNYTYVEACEGQDMMSWLNAHSRCLTFLGGVPKLLIPDNLRSAVKKADRY
                     EPVINDSYQALAEHYGTVIIPARPRKPKDKPKAENGVLIVERWLLARIRNETFHTLRA
                     LNARLRELLTDMNNRPMKGYGNQTRAERFRMLDAPALSPLPLEPYEYTEYKAVKVGPD
                     YHVEYARHWYSVPHELVGQRLSLKVGQSVVQLWHKGQCVAQHPRSTHEYKHTTNPLHM
                     PERHRRHGTWTPERLIEQGNRTGPSTGRVVESMLKAKPHPELAYRAVLGLLALQKKYG
                     PER"
BASE COUNT          397 a          437 c          392 g          340 t
ORIGIN      
        1 cagtttggca ttcgaatcga tcatgatttg gcatccatct ccgatcacag tttggcatca
       61 caatcgatca cgatttggca tacttccgat cattgattgg aattcgccag tcactccggg
      121 aattaactct tttcaccaca gtctccattg ccgtgtttaa accagtggag acgtcaatgt
      181 ccaaaaagag aaaatccagg agcactatgg aaacttgttt taagatcctt cagctcaagt
      241 tcgataagaa gctgactaac cgttgtatcg gactgacgtt acacatcagc gcctcaaccg
      301 tttttgaggt tcttgcccgc ttcaaagcct cttctctgtc atggccatta cctgcggata
      361 tatcccatga cacgctggag aaactcattt tcccgcccaa agacacctct gcgtcagagc
      421 ttgttatgcc tgacatgctg tacttcgata cggaaatgag aaaacctggc gtgacgcgcc
      481 aactgttgtg gatggaatac aaggcacagg cgggtgacaa agcgatggga tactcacact
      541 tctgccgttg ctaccggaag tggaaaaaaa cgcgacgcct ctccatgcga caggagcatc
      601 gtgctggcga aaagctcttt atcgacttct gtggtcctac ggtccctgtt attaatccgg
      661 acaccggaga gatacgccgc gtggcaatct tcgtggctgt aatgggggcg tccaactata
      721 cctatgttga agcgtgcgaa ggtcaggaca tgatgtcatg gttaaatgcc cacagccgtt
      781 gcctgacctt cctgggcggc gtaccgaagc tgctcatccc cgataacctc agaagcgcag
      841 tgaaaaaagc cgatcgctat gagcctgtca tcaacgacag ttatcaggcg ctggctgagc
      901 actatggcac ggttattatc ccggcccgcc cgcgcaaacc taaagataaa ccaaaagcag
      961 aaaacggcgt cctcatcgtc gagcgatggc ttcttgcccg catccgtaat gaaaccttcc
     1021 acacgctcag ggcgctgaat gcacgccttc gggagttgct gacggacatg aataaccgac
     1081 caatgaaggg atacggtaat caaacccgcg cagaacgctt ccgcatgctg gatgcaccag
     1141 cactctctcc gctgcctctg gagccttatg aatacacgga atacaaagcc gtgaaggttg
     1201 gccctgacta ccacgttgaa tatgcccgtc actggtactc cgttccacac gaactggtcg
     1261 gtcagcgtct gtcactgaag gtggggcagt cggtggtaca gctctggcac aaggggcagt
     1321 gtgtggcgca acatccgcgc agcacacatg aatataaaca cacaaccaat ccgcttcaca
     1381 tgccagaacg ccaccggcgg catgggacat ggacaccgga acgccttatc gagcagggaa
     1441 accgtacagg tccgtccaca ggccgggtgg ttgaatccat gctgaaagcc aaaccccatc
     1501 cggagctggc ttaccgtgca gtcctgggtc tgctggcact tcagaaaaaa tacggcccgg
     1561 aacggc
//
LOCUS       DAZPDU010000362         1531 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZPDU010000362 DAZPDU010000000
VERSION     DAZPDU010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.366"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            77..739
                     /locus_tag="ACHURS_004639"
     CDS             77..739
                     /locus_tag="ACHURS_004639"
                     /inference="COORDINATES: protein motif:HMM:NF017666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA N-6-adenine-methyltransferase"
                     /protein_id="HFR1240173.1"
                     /translation="MSIEHILIGKHHGGSDYWRTPFLLFKNLHREFIFSLDGAATEHD
                     TLLPRFTDDISRQSWVSEKVFCNPPYSDIPSFLLKASEADLVAFLIPHRANTSYWLRH
                     IYSNNHCHEIRILHRAVKYLPPAGHNRLTIRSPFPSAVVVFKKEPRKHEITQMVCCAD
                     TLLPLTIINRGGLRGRPTIYPPETLDSFIKLYRQGKPIKYIADALRMPLSTSYRIAQR
                     LS"
     gene            1103..1520
                     /locus_tag="ACHURS_004640"
                     /pseudo
     CDS             1103..1520
                     /locus_tag="ACHURS_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049841.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          480 a          303 c          303 g          445 t
ORIGIN      
        1 agaaaacctg ttaagtatct cttaaaagta gagtaaacct tgtcaagagt gattgcaata
       61 aaaattttta tttcttatgt caattgagca tattttaatc gggaagcatc atgggggttc
      121 tgattactgg agaactccat ttttgttatt taagaactta caccgtgaat ttattttcag
      181 tctagatggt gctgcaactg agcatgacac gctgttacca cgattcaccg atgatataag
      241 ccgtcaatca tgggttagtg aaaaggtctt ctgtaacccg ccgtacagcg atattccgtc
      301 gttcctctta aaagcgtctg aagcggattt agtagctttc ctgataccgc atagagccaa
      361 caccagctac tggcttaggc acatttacag caataaccac tgtcatgaaa tccgtattct
      421 tcatcgtgca gttaaatacc ttccaccagc agggcataac aggcttacta ttaggtcacc
      481 gtttccaagt gcagtcgttg tttttaaaaa agaaccacgt aagcatgaaa ttacacagat
      541 ggtttgctgt gctgatacat tgctaccgtt aaccatcatt aaccgtggtg gactaagagg
      601 aagacccacc atttacccac ctgagacgct cgacagcttc ataaaactat acaggcaagg
      661 aaaacccatt aagtacattg cagatgctct cagaatgcct ctgagcacgt catacaggat
      721 tgctcaaagg ttgagctaaa attctcataa atatggtcat ttacaagaca ggtgattcag
      781 atgttaagaa gtatccctgt gaacaataaa tgaccaatca gtaatcattc tgtgaaataa
      841 ttgttgcaat ttataatcac atctgttata gtttctatat gggctgataa aaaagttcat
      901 gtcttgaagt ggtaacactt ggggaggtgt cagatggttg gcgtatatta acctctcatt
      961 cagggtatac ctcagcgttt taatcatcgt actgtttcag gcatggactt ctttatcagt
     1021 ccatcaatcc cccagatatt ttaatcacac aggctaatcg gttttatccg gttggccttt
     1081 tcacgtatga ggtaatttat gaatgtctaa atataaccca aatcagtttt ataaatttga
     1141 tgtgcgaatc tctcaacttt cagaagaaat taacgcagca gcaagattga tttatatggt
     1201 catggtccat agctgtgact tctatgaaga gaaatacaaa tcatattcac caagtattga
     1261 ccatctcgct tatatggctg gtgtatcaaa gagaacagca atcagagcca ttgagacact
     1321 tgagaaacac ggtttaattc aggtgcaaaa gaatcagggg cgtcaaagca cttatcgtgt
     1381 attcacactg gatgataagc cggaattact tgtaagacct acatcacagg aagttgcagc
     1441 ctcagtaaga caaaatgaca ccactaccag tgacaaaatg tcatgggggt agtgacaaaa
     1501 tgtcacccgt gttagagaga taagttatta g
//
LOCUS       DAZPDU010000363         1527 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZPDU010000363 DAZPDU010000000
VERSION     DAZPDU010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.367"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            99..362
                     /locus_tag="ACHURS_004641"
                     /pseudo
     CDS             99..362
                     /locus_tag="ACHURS_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701870.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
     gene            complement(399..956)
                     /locus_tag="ACHURS_004642"
     CDS             complement(399..956)
                     /locus_tag="ACHURS_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR1240174.1"
                     /translation="MNTAITFSNESPKVFFKDGRLVTTSQAVADYFDKQHKHVLAKID
                     TLDCSPEFTSANFSADVQTVEIGNGAERESRCYLITKDGFMFLVMGFTGKKAARLKEA
                     YIEKFNAMEEELHKRPPAAQNSPAPNNGCALLIHFDKHGQVEFTEKVPADAMVCTLER
                     FKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(953..1228)
                     /locus_tag="ACHURS_004643"
     CDS             complement(953..1228)
                     /locus_tag="ACHURS_004643"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HFR1240175.1"
                     /translation="MTVFVLRGYIPHTTAKSVVGIGVSDNITTHSRVCGFFCVKHCHI
                     RIMVGRAGAEKSAPGFVMTGYANPVRLTTSLIGVRGGDHPINWSHHI"
     gene            1414..1527
                     /locus_tag="ACHURS_004644"
                     /pseudo
     CDS             1414..1527
                     /locus_tag="ACHURS_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
BASE COUNT          424 a          326 c          368 g          409 t
ORIGIN      
        1 gccgcagaca cgtcgtatgc aggaacgtgc tgcggttagt ttgtgagctt tcgatagtgg
       61 ttgttatttt tgcccttatt tgttccggag gccatggttc aatggtccgt ctgccccctg
      121 tggtgatgtc agcaaaatca gccactgcgc gaaccacaat agcccgggaa gatgctgaag
      181 atcaccaggt aaagctgtca gcgcagaaac tggaagaact gctcgcatca atggttaagg
      241 atgaggttga tcgcaatgat gggatttatt gacgtcagcg agagcagaag gaagaactga
      301 ataacctgaa tgatttacgc tcaatcagag cgatggcgat tagcggcaat cacgctcgat
      361 aaaattaacc tcggtgatca tccggggttt tgcattcatt aaaaccgcat caacctttcc
      421 accaattgtt ctttacgggc aacgatccac ccatgttgct ccagataaaa tttaaaccgt
      481 tccagagtgc ataccatcgc atcggcgggg actttttctg tgaactcgac ctgaccgtgt
      541 ttatcgaagt ggatcagtaa tgcgcatcca ttatttgggg cgggggagtt ttgtgctgca
      601 ggaggacgtt tgtggagttc ttcttccatt gcgttaaatt tttcaatgta agcttctttt
      661 agtctggctg cttttttgcc agtaaatccc attacgagga acatgaagcc gtcttttgtg
      721 atcaggtagc atcgtgattc acgttctgcg ccgttgccaa tttcgacagt ctgaacatcg
      781 gccgaaaagt tggctgatgt aaattcggga gagcagtcga gtgtgtcaat cttagctaaa
      841 acgtgtttat gttgtttatc gaagtagtcg gcaacagctt gagaggtggt aaccaggcga
      901 ccatctttga aaaagacttt tggtgactcg ttagaaaaag ttatagctgt attcatatgt
      961 gatgactcca attaattgga tgatcaccac ctctgacgcc aatcagggag gtggtgagac
     1021 gtacagggtt ggcgtaaccg gtcatcacaa aacccggcgc ggatttctcc gcccctgcac
     1081 gccccaccat aatgcgaatg tggcaatgct taacgcaaaa aaaaccgcaa acgcggctat
     1141 gcgttgtgat gttatccgag acgccaatcc cgacaaccga ttttgcggtt gtgtgaggaa
     1201 tatagccccg gagaacaaaa actgtcaacg ggcaaaaaaa tttccgttat caggaatgta
     1261 taaaattaaa gcgatcgagc aaaaaatcct ttctgcaaaa ctaaaaaaga tttcaataaa
     1321 atcaggcagt taaaaacacg gaaaaacggt ataaaaaagc gcctcaaaat cctgtttttt
     1381 ctttcagata caatgtctta ttgcattttt taaaacatgt cagtgtggta catggatatc
     1441 gataccatcg ccaattataa actgggcggt gcacagcaac atgatagcgt acgcctcgat
     1501 ccgtgggtgt ttatgttctc agcagga
//
LOCUS       DAZPDU010000364         1507 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZPDU010000364 DAZPDU010000000
VERSION     DAZPDU010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.368"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..207
                     /locus_tag="ACHURS_004645"
     CDS             <1..207
                     /locus_tag="ACHURS_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240176.1"
                     /translation="DAKRAKADLLEKTFTSRSWERIKGMDWPTIKAGRNALWIELEGV
                     EYAFPDPQTQNETQQAGYDENIPV"
     gene            258..446
                     /locus_tag="ACHURS_004646"
     CDS             258..446
                     /locus_tag="ACHURS_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFR1240177.1"
                     /translation="MYKITATIEKEGGSPASWTRYSKTKLTKSECEKMLSGEKEAGVS
                     REQKVKLINFNCEKLQSS"
     gene            546..761
                     /gene="xisR"
                     /locus_tag="ACHURS_004647"
     CDS             546..761
                     /gene="xisR"
                     /locus_tag="ACHURS_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase XisR"
                     /protein_id="HFR1240178.1"
                     /translation="MAQVIFNEEWMVEYGLMLRTGLGARQIEAYRQNCWVEGFHFKRV
                     SPLGKPDSKRGIIWYNYPKINQFIKDS"
     gene            763..>1507
                     /locus_tag="ACHURS_004648"
     CDS             763..>1507
                     /locus_tag="ACHURS_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HFR1240179.1"
                     /translation="MSKLPTGVEIRGKYIRIWFMFRGKRCRETLKGWEITNSNIKKAG
                     NLRALIVHEISSGEFEYLRRFPQSSTGAKMVTTRVIKTFGELCDIWTKIKETELTTNT
                     MKKTKSQLKTLRIIICESTPISHIRYSDILNYRNELLHGETLYLDNPRSNKKGRTVRT
                     VDNYIALLCSLLRFAYQSGFISTKPFEGVKKLQRNRIKPDPLSKTEFNALMESEKGQS
                     QNLWKFAVYSGLRHGELAALAWEDVDFEKG"
BASE COUNT          523 a          279 c          347 g          358 t
ORIGIN      
        1 gacgccaaac gcgcaaaagc tgatttgctg gaaaaaacat tcacctcccg ctcatgggaa
       61 agaatcaaag gtatggactg gccgacaatc aaagctggtc gtaatgcact atggattgaa
      121 ctggaaggcg ttgaatatgc attcccggat ccacaaacac aaaacgaaac gcaacaagca
      181 ggatacgatg agaatattcc ggtgtaaaac caagtgcatt ttggacacgg tttttgaaaa
      241 tattaatgga ggtgttcatg tataaaatta cagccactat tgaaaaggaa ggtggttctc
      301 ctgccagctg gacaagatac tcaaagacaa aactaacgaa atcagaatgc gaaaaaatgc
      361 tctcagggga aaaagaagca ggcgtttcca gagagcagaa agtaaaactg ataaatttta
      421 attgcgagaa acttcagtcc tcgtgaattg cattgtattc aaattaaaac ttcatagctg
      481 attattaata atcaacatcg ggcgtcaatt tcagcctaac attggcgcct gccagaggtg
      541 atgcgatggc acaagtaatc tttaatgaag agtggatggt tgaatacggc ctgatgcttc
      601 gcactggtct gggtgccaga caaattgaag cataccgcca gaactgttgg gtggaaggct
      661 tccacttcaa acgagtatct cctttaggga agccagacag taagcgaggg attatctggt
      721 acaactatcc gaagataaat cagtttatca aagactcatg atatgtctaa attaccaaca
      781 ggtgtcgaga ttcgaggtaa atacattcgc atctggttca tgtttcgagg aaaacgatgt
      841 cgggaaacat tgaaaggctg ggagattact aacagtaaca ttaaaaaggc cgggaattta
      901 agagcgttga tagttcatga aatcagttcc ggtgaatttg agtatttaag acgttttccc
      961 cagtccagca ctggggcaaa aatggtgaca acgagggtca taaaaacatt cggggagctt
     1021 tgtgatatct ggacaaaaat taaagagaca gagttaacaa caaacacaat gaagaaaacg
     1081 aaatcacaat taaaaacact caggataata atttgtgaga gtaccccgat atcgcatatt
     1141 cgttatagcg atatcttaaa ctaccggaat gaactgctgc atggagaaac gctttaccta
     1201 gataatccaa gatccaacaa aaaaggaaga accgtgcgca cagttgataa ctatatcgcc
     1261 ctgctctgtt cgttgttacg ttttgcgtat cagtcgggat ttatatcaac caaaccattt
     1321 gaaggagtaa aaaaattaca gcgaaacaga ataaagcctg acccgttatc taaaacagaa
     1381 ttcaatgcat taatggaaag tgaaaaagga cagagccaaa acttgtggaa atttgccgtt
     1441 tactccgggc ttcgtcacgg ggaactggca gctctggcgt gggaggatgt ggatttcgag
     1501 aagggaa
//
LOCUS       DAZPDU010000365         1482 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZPDU010000365 DAZPDU010000000
VERSION     DAZPDU010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.369"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..1012)
                     /gene="cadA"
                     /locus_tag="ACHURS_004649"
     CDS             complement(<1..1012)
                     /gene="cadA"
                     /locus_tag="ACHURS_004649"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFR1240180.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYT"
     gene            complement(1092..1460)
                     /gene="cadB"
                     /locus_tag="ACHURS_004650"
                     /pseudo
     CDS             complement(1092..1460)
                     /gene="cadB"
                     /locus_tag="ACHURS_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="antiporter protein responsible for lysine import
                     and cadaverine export; member of the lysine-dependent acid
                     resistance system 4 (AR4); inner membrane protein;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
BASE COUNT          414 a          324 c          370 g          374 t
ORIGIN      
        1 tggtgtaagg cacccacgcg gagtcaaagt ggatggattt cacatccagt gttttcttga
       61 tgaagtcggt gttgtacagc agaccatcat aggtagagtt ggtaattaca gcatgtaccg
      121 gccaggttgc gtttggtgtt tctttcacgc gcttagcaat ggtagcgtgc tggaattcac
      181 tctgtgggat accaccaaga ataccgtaag cgttacgggt cgggcggaaa tagattggcg
      241 taacatcgct catcatcatc aggtgggtca gcgatttgtg gcagttacgg tcaatcagaa
      301 tggtgctgcc tgccggagca gagtacatgc caacaatttt gttcgcagtg gaagtaccgt
      361 tggtcaccat gtagctgcgg tctgcgttaa agacgcgagc gatatactgt tctgcttctt
      421 tgtgtggacc actatgatcc agcagagaac ccagttcaga tactgaaatg gaaatatcag
      481 atttcatggt attcggacca aagaaatcat agaacaggct acctaccggg cttttctgga
      541 atgcagtacc gcccatgtga ccaggagtac agaaagtata tttaccttca cgaacatatt
      601 taaacagtgc tttagtcagc ggaggcagaa tagtgttgat atattcgtca gtggtctgct
      661 tgatcttatt agcaatatct tcagcagcac ccagcgcata ttcaaagaag ctaatctgta
      721 aacgcaggtc attcaggctt acatcgagag tggaatacgt attagcgaac gcgtacaacg
      781 gcaggttctc gttcattttg ctaatttctt cgcacagctc gagattatat ttatcccagt
      841 caaaaataac gccgcacaga cgcgcattgt tttcgatcag ttttaataag tcgtcacggt
      901 cgttcgggta aacaatctgg aagttcagac gttcaagcgc gcgatgaagt tcacggatgg
      961 gttcttcttt aaaataaacc cccatgtgat tcaatattgc aataacgttc atagtcatat
     1021 ctccaggtaa aaaaggcccc tcccaacaca tgggacaaaa tgaaaggagg agcctcggaa
     1081 aatactttta attaatgtgc gttagacgcg gtgtggttat ccattgagtg gctctggcgc
     1141 tcgtgcattt tgcgagcgta gaacatcagg ataatcaggc tgacgatgaa ggtacctgcc
     1201 agctcgaagg agcttgcgcc catcagcgcg atgaagcaga acacacaacc cagtacagag
     1261 cagatcaggc tgacaaagtt gcgaatgtta acgccttcaa aacgaatcag gtcaacgcaa
     1321 gagtagaagt acggcagcat agtcagcagt actgcgatac cggtcagttc accgaacagg
     1381 tcagatgctt taccaccggc agagttcatc agagtgatga ggatcatcag ggcagtcatt
     1441 ttcactgcag ccagcagcag taatgccgat cagttaagga tc
//
LOCUS       DAZPDU010000366         1471 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZPDU010000366 DAZPDU010000000
VERSION     DAZPDU010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.370"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..122
                     /locus_tag="ACHURS_004651"
     CDS             <1..122
                     /locus_tag="ACHURS_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240181.1"
                     /translation="HFLPVDQESARVLSTPMKPGNPRRYSNKRTDSSKMERFF"
     gene            119..376
                     /locus_tag="ACHURS_004652"
     CDS             119..376
                     /locus_tag="ACHURS_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240182.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEVLR"
     gene            376..750
                     /locus_tag="ACHURS_004653"
     CDS             376..750
                     /locus_tag="ACHURS_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR1240183.1"
                     /translation="MRHEFILPYPPTVNTYWRRRDNTYFVSKAGERYRRDVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMHEEQVKK"
     gene            747..>1471
                     /locus_tag="ACHURS_004654"
     CDS             747..>1471
                     /locus_tag="ACHURS_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR1240184.1"
                     /translation="MKLEDLPKYYSPKSPCLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESVVFTNKVQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRAS"
BASE COUNT          406 a          312 c          424 g          329 t
ORIGIN      
        1 gacattttct gcctgtggac caggaaagtg cacgggtgct ttccactccc atgaagcccg
       61 gcaatccgcg cagatacagt aataaacgaa ctgacagcag taagatggag cgtttctttt
      121 gaacaaccag acaatgactt ttacccctga acaattgcgt aaacacgcgc aggaaatgct
      181 gcgtcatgct gaacaactcg aaaaaacggg cataacaaaa gacgctatcc gtaaagatat
      241 ggtacccgcg cttcgggaac tgatgcaggc gaagcatcgc gcacagaaag cagtagatga
      301 gctggtggac tgtgtggcag agctggaaac cagagtcgga aagtttgaaa aactggtgca
      361 ggaggtgctg cgctgatgcg ccatgagttt attttacctt atccgccgac ggtgaatact
      421 tactggcgac gtcgtgacaa cacatatttt gtatcaaaag ccggtgagcg ttatcgccgg
      481 gatgtggcgc ttattgtccg tcagcagcga ctgaaattaa gcctgtccgg aagactggcg
      541 ataaagatta ttgcagagcc accggataag cgccgtcgtg acctggacaa tatcctgaaa
      601 gcaccactgg atgcactgac acatgcggga gtgttaatgg acgatgagca gtttgatgaa
      661 atcaatatcg ttcgtggtca gccagtatct ggtggacgtc tgggggtgaa gatttacccc
      721 ataatgcatg aagagcaggt caaaaaatga aactggaaga tttaccgaaa tactactccc
      781 caaaatcccc ttgcctgacc gatgcatcgg cctcaacgtc aaaagatgcg ctgagtatca
      841 ctgatgtgat ggccgcgcag ggcatgacac agaatcgggc tgagatgggg ttttctgcgt
      901 tcctggggaa aatgggcatc agtatgaatg acagggcgcg ggcaacagaa ttactggcag
      961 attatgcact cagtcggtgc gatcgtgtgg cggcgttgag aaaacttccg gcagaaataa
     1021 aaccggtagt gatgcgcatt atggcttcgt acgcttttga ggattatgcc cgcagcgcag
     1081 cgagtaaaaa gcagtgccct tgttgctatg gggaaaaatt tattgaaagc gtagttttta
     1141 caaacaaggt ccagtatccg gatggtaagc cgccggtatg ggcaaagtgt acgaaaggtg
     1201 tgtatccgtc ttactgggaa gaatggaaaa aagtcaggga ggtggtaaaa gttgcctgtc
     1261 cggagtgtgg cggaaagggt gaggtttcca ccgcctgtaa ggattgccgt gggcgtggtg
     1321 tcgccattca tcgtgaagag tcggtaaaac gtggtatgcc tgttatcaga gactgccagc
     1381 gttgtggtgg tcgtggctat gaaagactac catcaacgga ggcatttaat gctatatgcg
     1441 aggtgacaaa ccagataaca cgcgcgtcat g
//
LOCUS       DAZPDU010000367         1441 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZPDU010000367 DAZPDU010000000
VERSION     DAZPDU010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.371"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..1234
                     /locus_tag="ACHURS_004655"
     CDS             <1..1234
                     /locus_tag="ACHURS_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFR1240185.1"
                     /translation="VKLIPQMNYLMVVVALFFLNAVIFLFMLMKYFTNKQILPTLILS
                     LAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVSIFYIFRQLSFICLTSLALFCYGK
                     DNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYNADYSLYVVDYCPDNHTATWGINY
                     TKILVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTLDEYNYTIWYISR
                     GIEVSSKLFVVSFLIYNIFQKLQLSSKLAVHDVLTNIYNRRYFFNSVESLLSRPVVKD
                     FCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQQSIRPDDILARLEGEVFGLLFTE
                     LNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDTRNISLVMTEADK
                     ALREAKSEGGNKVIIHHI"
BASE COUNT          389 a          304 c          282 g          466 t
ORIGIN      
        1 tgtaaaactc atcccacaga tgaattacct gatggttgtt gtggctttgt ttttccttaa
       61 cgccgtcatt tttcttttca tgttaatgaa atatttcact aacaaacaaa ttttaccaac
      121 actcatttta agccttgcat ttttaagtgg ccttatctat ttagttgaaa ccattgtaat
      181 tatccataaa ccaattaacg gcagtacact gatccagaca aagtcgaatg atgtttctat
      241 tttctatatt ttccgccaac tcagttttat ttgtttaacc tcgctggcac tcttttgtta
      301 tggaaaagac aacatccttg acaacaataa gaaaaaaacg ggaatcctgt tgctggcgct
      361 gattcctttt ttagtttttc cccttctggc acacaatctg agcagttata acgctgacta
      421 ttctttgtat gtcgtcgact actgtccgga caaccatact gcgacctggg gaatcaacta
      481 tacaaaaata ttggtttgtc tgtgggcatt tttactgttc tttattatca tgcgtacacg
      541 attagccagc gaactatggc cgttaatagc attattatgt ctggcatcgc tatgctgcaa
      601 cttacttcta ctgactctgg atgagtataa ttacaccatc tggtatatca gtcgcgggat
      661 tgaagtttcc agtaaactgt ttgttgtgtc ttttctgatt tataacattt ttcagaaact
      721 gcaactctcc agcaaactgg cagttcatga tgtgctgacc aatatttata atcggcgcta
      781 ctttttcaac agcgtagagt cattattgtc gagacctgtt gttaaggact tctgtgtcat
      841 gctggttgat attaatcagt tcaaacgcat caatgcccaa tggggacatc gtgtgggtga
      901 taaagtgctg gtttcaattg tcgatattat ccagcaaagc atccgccccg atgatatttt
      961 agcgcgactg gagggtgagg tgtttggctt gctatttacc gaactcaata gtgcccaggc
     1021 aaaaatcatt gcggaacgta tgcgtaaaaa tgtcgaactc ctgaccggct ttagtaacag
     1081 atatgatgtt cctgaacaaa tgaccatcag tattggcacg gttttttcaa cgggtgacac
     1141 gcgtaatatc tcgcttgtca tgacggaagc agataaagcc ttacgcgaag cgaaaagcga
     1201 ggggggcaac aaagtgatta ttcatcatat ttaagtgcaa aaatattcag agccatgctt
     1261 tttgcgtggc ttttgcatac aatttattac tattacccct aaatttcccc tccccagcgg
     1321 cgcagagatg agtataatta gcgcccctgt gccaggccgc aatcgaactt tatctggttt
     1381 tctcgtttca ctaaccgaag gagtgccatt tatcatgaaa ttgcaccata gaatgctccg
     1441 g
//
LOCUS       DAZPDU010000368         1437 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZPDU010000368 DAZPDU010000000
VERSION     DAZPDU010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.372"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..1104
                     /locus_tag="ACHURS_004656"
     CDS             <1..1104
                     /locus_tag="ACHURS_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR1240186.1"
                     /translation="LAWAVFKIFRIPVNQWTLATAALGGVFLVSGLILLMNYNHPYTF
                     TAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTA
                     THNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNF
                     LAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGY
                     VTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPG
                     QVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYA
                     QVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
BASE COUNT          349 a          327 c          385 g          376 t
ORIGIN      
        1 ctggcgtggg cggtctttaa aatctttcgt attccggtga atcagtggac gctggcgacg
       61 gcggcgctgg gaggcgtgtt tctggtgagt ggtttgattt tgttgatgaa ctacaaccac
      121 ccttacactt ttaccgcgca aaaggcagtg atagcgatcc ctatcacgcc acaggtgacg
      181 ggaattgtta ctgaagtcac tgacaagaat aatcagctta ttcaaaaggg cgaggtgctt
      241 tttaagctcg acccggttcg ttaccaggcg cgagttgaca gacttcaggc tgacctgatg
      301 acggcgacgc ataatataaa gacgctgcgt gcgcagctca ctgaagcgca ggccaacacc
      361 acccaggttt cagcggagcg cgaccgtctg tttaaaaatt atcaacgtta cctgaaaggc
      421 agccaggcgg cggtgaatcc gttctcggaa cgtgacatcg acgatgcgcg gcaaaatttc
      481 ctcgcgcagg atgcgctggt gaaaggctcg gtggcggagc aggcgcagat ccagagccag
      541 ctcgacagta tggttaacgg cgagcaatcg cagattgtga gcttaagagc gcaacttact
      601 gaagcaaaat ataaccttga gcagactgtc attcgcgcgc cgagcaatgg ctacgttact
      661 caggtactga tccgcccagg tacatacgca gctgccttgc cgctgcgtcc ggtgatggtc
      721 ttcatccccg agcaaaaacg gcaaattgtc gcccaatttc ggcaaaactc gctgttacgt
      781 ctgaaacctg gcgatgatgc ggaagtggtg tttaacgcgc tacctgggca ggtgttccac
      841 ggcaaattga ctagtatttt acctgtcgtg ccaggcggtt cttatcaggc gcagggggta
      901 ttgcaatcat taacggtcgt tcccggcacg gacggcgtgc tgggaaccat tgaactggac
      961 cctaacgatg atatcgatgc cttacccgac ggcatttacg cccaggtagc agtttactcc
     1021 gaccatttca gccatgtttc ggtgatgcgg aaggtgctgc taagaatgac cagttggatg
     1081 cattatttgt atttggatca ttgaggggga agggggagat tatggaactt tgctccctgc
     1141 aagggctaat tatttttgca tattacttca ttggctgcga tgatttttct ttaatattca
     1201 ttatgttaat aagttctgga tgagtgtgct ctcattggat tttttatccg tcttgttctg
     1261 ccgcaggatg agactataca tcgtcgagat cgcaatgatg atatgagttg ttcctcctgc
     1321 gacggctcta atcaaaaaag agcaggagtg cttttccatc tattgcgatg atggctttat
     1381 tatctgaagg atgacagtca catttaaaaa atatttaatc tatagatcac aatgtag
//
LOCUS       DAZPDU010000369         1432 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZPDU010000369 DAZPDU010000000
VERSION     DAZPDU010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.373"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..661)
                     /locus_tag="ACHURS_004657"
     CDS             complement(<1..661)
                     /locus_tag="ACHURS_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HFR1240187.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NG"
     gene            complement(825..920)
                     /gene="ymiC"
                     /locus_tag="ACHURS_004658"
     CDS             complement(825..920)
                     /gene="ymiC"
                     /locus_tag="ACHURS_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFR1240188.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(1034..1201)
                     /gene="yciX"
                     /locus_tag="ACHURS_004659"
     CDS             complement(1034..1201)
                     /gene="yciX"
                     /locus_tag="ACHURS_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFR1240189.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(1204..1344)
                     /locus_tag="ACHURS_004660"
     CDS             complement(1204..1344)
                     /locus_tag="ACHURS_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFR1240190.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
BASE COUNT          382 a          350 c          334 g          366 t
ORIGIN      
        1 ggccgttgat catagtggtg tgcgagtcag taccaacgag tgtatccgga taagcaatcc
       61 attcaccgtc ctgcaattca ctccacactg ctttgccgag atattcgagg ttaacctgat
      121 ggcaaatgcc tgtgcctggc ggcacgacgc taaaccgact gaacgcttgc tttccccatt
      181 tcaggaacac ataacgttcg tggttgcgct ccatttccag gcgtacgttt tcttcaaatg
      241 cctcatcatc accaaaacga tcgacggtca ccgagtggtc aatgaccagg tcgaccggtg
      301 agagcgggtt aacctttgca gtatcgccgc cgaggcgttt aaccgcttcg cgcattgccg
      361 ccagatcaac aacggcaggt acgccggtaa agtcctgcat caacaccctt gccgggcggt
      421 aggcaatttc acggtcagca tgggcatttt tcagccatcc tgccagcgcg tggatatcct
      481 cttcggtaac cgagttacca tcctgccagc gcagcaggtt ttcgagcaaa actttgagtg
      541 acttgggtag acgggtgata tcgcccagtg atttagcagc aagcggcagg ctgtagtagt
      601 ggtaagtttt atctttggcc tgcaacgtgt ccttactggc ttctcgtagg gttgacgaca
      661 tagctcctcc ttaatgacag ggttgcgaag ccctgattct cttcagggtg aatattaaag
      721 ataacacaaa catcgcgtaa cgatttgata acaacccaaa ttgataaaag aggagaaacc
      781 ttgtggcaaa gcaaatgaca accccgccgc agcggggtca aggatcagtg agtaatgatc
      841 cagaggagtt cggcccagaa gagcatcgac agagaaaacg cgcccatcca ggaccaatat
      901 ttcatatttg tgttgatcat aggctacacc cttaacggtt attattttgt gttgagataa
      961 ctcaagctgt tattgctgcg gtgtattaaa agccctttaa ttcattaaaa atgggtaata
     1021 agaatgatga taattattgt ttcggctgat catcttcagc aaacagttct ataaaggctt
     1081 gctgctgcga tgttagttgc cagtcgtctg gaataacgac ggaacattcc cgtttcgttt
     1141 ctgtttcatt gtcactatgc tggcataatg gtgacgactc cagttgctcc tgaccgacca
     1201 tagttatccc cacactttcc aaatcagcag tgcgacaacc acccagaaaa gtgcagaacc
     1261 aagaaaaacc gccagccagg ctttacgttt taattcagga tcgcggcggg gttcctgatt
     1321 tccagaaggc attgctaacc tcatataatc gccatcactt atcagcaaga cgttgaacga
     1381 ttggtgtaat taatcatacg atgagacaaa tcctaaagaa actttagtca aa
//
LOCUS       DAZPDU010000370         1429 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZPDU010000370 DAZPDU010000000
VERSION     DAZPDU010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.374"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            149..310
                     /locus_tag="ACHURS_004661"
                     /pseudo
     CDS             149..310
                     /locus_tag="ACHURS_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300548.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
     gene            288..584
                     /locus_tag="ACHURS_004662"
     CDS             288..584
                     /locus_tag="ACHURS_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFR1240191.1"
                     /translation="MSAYPIFDRIEEMAWARHYQQIVREEKETELADDLEKGLPQHLF
                     ESLCIDHLQRHGANKQAISHAFDDDVEFQERMAEHIRYMAETIAHHQVDIDLED"
     gene            590..>1429
                     /locus_tag="ACHURS_004663"
     CDS             590..>1429
                     /locus_tag="ACHURS_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR1240192.1"
                     /translation="MALFQRAINTQAYLKAGIMGFAGDGKTYTASELAIGLVMLMRQR
                     GIEAGNRPVMFLDTETGSDWVKPRFDAENIELFTAKTRSFVDLLEAINEAESSGSVMI
                     IDSISHFWTGLCDEYARRRNRKRGLEFSDWAWLKQEWRRFTDRFVNSQAHIIMCGRAG
                     YEYDFFEGDDGKRQLAKTGIKMKAETETGYEPSILIQMEKQMNIETGQVWRTARILKD
                     RSTRIDGQVFSNPTFKNFLPHIEFLNLGGEHLGIDTSRDNSELFANDGTPTWLKEKRA
                     KEIA"
BASE COUNT          417 a          320 c          350 g          342 t
ORIGIN      
        1 gaaccgttct gacagcttcg gctttgaact ctttagagta acgttgggtt tttctgctca
       61 ttattagctc cttctgatgc cattctattt caggaaggag tgtccgttaa actcaggcta
      121 cctcaataat gaaactggcg aagatgaact caactggaaa aaacaggtgt tgattgcggc
      181 ggtttacggt ttatgtgcga atcctgcgtg catagcctat gcgcctgcaa tccctgacat
      241 cgcaatgatg attgccaaca agcttgaaaa ttttggagtg accagtgatg agcgcttacc
      301 cgatttttga tcgtattgaa gaaatggcat gggctcgcca ctaccagcag atcgttcgtg
      361 aagaaaaaga aacggaactg gcggacgacc tggaaaaagg tctgcctcag cacctgtttg
      421 aatcgctctg catcgaccat ttgcaacgcc acggtgccaa taaacaggca atcagccacg
      481 catttgatga tgatgttgag tttcaggagc gcatggcaga acacatccgg tacatggctg
      541 aaaccattgc ccaccaccag gttgatattg atttggagga ctaaaatcta tggcgctttt
      601 tcaacgagca ataaacacac aggcctatct aaaagcagga attatggggt ttgctggcga
      661 cggcaaaacc tacacagcaa gcgaactggc tattggtctg gtaatgctta tgcggcaacg
      721 gggaattgaa gctggtaacc gcccggtaat gtttctggac acagaaacag gctctgactg
      781 ggttaaaccc cgctttgatg cggaaaatat cgaattattt acggcaaaaa cccgctcgtt
      841 tgttgatctg cttgaagcca ttaatgaagc ggaaagcagc ggttccgtaa tgatcatcga
      901 ctccatcagt cacttctgga ccggattatg cgacgagtat gccagacgcc gaaaccgtaa
      961 acgaggactg gaattttcag actgggcgtg gctcaaacag gagtggcgac gctttaccga
     1021 tcgatttgtt aacagtcagg cgcacattat tatgtgcggg cgtgcaggat atgagtatga
     1081 ctttttcgaa ggtgatgatg gcaaacgcca gttggcaaaa accggtataa aaatgaaagc
     1141 agaaacagaa accggttatg aaccctccat cctgatccag atggagaagc aaatgaacat
     1201 agaaaccggt caggtgtggc gcaccgccag aatactaaaa gatcgctcca cccgtattga
     1261 tggacaggta ttttcaaacc caacatttaa aaactttctt cctcatatcg agtttcttaa
     1321 cctgggaggt gaacacctcg gcatcgatac ctcacgggat aacagtgaat tatttgccaa
     1381 cgacggtaca ccgacatggc taaaagaaaa gcgtgcaaaa gaaatcgca
//
LOCUS       DAZPDU010000371         1429 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZPDU010000371 DAZPDU010000000
VERSION     DAZPDU010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.375"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..119)
                     /gene="rutC"
                     /locus_tag="ACHURS_004664"
     CDS             complement(<1..119)
                     /gene="rutC"
                     /locus_tag="ACHURS_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFR1240193.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQH"
     gene            complement(131..823)
                     /gene="rutB"
                     /locus_tag="ACHURS_004665"
     CDS             complement(131..823)
                     /gene="rutB"
                     /locus_tag="ACHURS_004665"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFR1240194.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            complement(823..>1429)
                     /locus_tag="ACHURS_004666"
     CDS             complement(823..>1429)
                     /locus_tag="ACHURS_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFR1240195.1"
                     /translation="KGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFC
                     FGKGVNTPTAFAPTAVRMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGAD
                     EEALSWLTEQSQKDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVP
                     GAEGVLLTFDDFLSGIETFGERIQPLMQCRAHLPALTQEVA"
BASE COUNT          324 a          397 c          396 g          312 t
ORIGIN      
        1 ttatgttgat caaaagccag cgtaccggag acatacacca cgccatcagc cagcgtgccg
       61 ggaacgaagg gggccagcgg tgcgctgctg ccagcgggaa taattacgga ttttggcatc
      121 gttaaactcc ttaagcgata cgagcaaagg acgtgggaga aagcgcgtcg cagaacgttt
      181 cgacgtcgct gacccagcca aaaaaggttt cgatattgaa caacgcggct ttctgtgcaa
      241 attcaggccc cgcctggtgt gttgcgtctt ccagcaccac gccgaaatac tccagaaaaa
      301 agccatcgcg tagcgtcgat tcgacgcaga cgttggtagc gataccggtg aaaaccagat
      361 ggcgtattcc acggctgcgc aaaatgctgt ccagcggcgt attgaagaaa ccgctgtagc
      421 gcggcttcgg cagcacaata tcgccaggct gcggcaccag ttcatccacc agttgataat
      481 cccaggagcc tttcgccagc aatttcccct gcagctgcgg ctgcttacgc atggttttca
      541 gggcgttcga tttatgaaaa ttcggtgagc cgggtccgcc agcttcgaca tactgttcat
      601 cccagccatt ttgaaaccag atgatcagca tccctgccgc tcgcgctgcg gtcacggcgg
      661 tttgaatgtt ggcaatgacc gggcgagtgg ttgagacatc aaacccggcg agatctaagt
      721 agccgcctgg cgtggcataa gcgttttgca tatccaccac gatcagcgca ctttgctgcg
      781 gatcgaaggt aatggcttcc ggtcgagcgg ttaaggtcgt catcatgcca cctcctgagt
      841 cagcgcaggg agatgggcgc ggcactgcat cagtggttga atgcgctcgc cgaaggtttc
      901 gattcccgac agaaaatcat cgaaggtcaa cagcacgcct tcggcaccag gcacgcttgc
      961 gacttcatct aacatgcgcg cgacactggc gtaagaaccg actaatgtcc ccatattgat
     1021 gtttaccgcc gaagtgggat cggccatctg acggacgttg gtgtcagtac cggagcgggt
     1081 atctttctga ctttgttcgg ttagccagct taacgcctct tcatccgcgc ccgctttgta
     1141 gtgttcccat ttggcgcgag cggcatcgtc ggtttcatcg gcaatcacca taaacaacac
     1201 ataagagcca acgtcgcgcc cggtttgctc tgcggcctgt ttcatccgca cagcggtcgg
     1261 ggcgaaagcc gtgggtgtat ttacgccttt gccgaaacag aagttgaaat cggcatactg
     1321 agcggagaac gccatgccag cgtcgctttg cccggcgcag atcactttca tggggacact
     1381 cggttgcgga ctgacgcgac aatcattcat ggtgaaaaaa tcgccttta
//
LOCUS       DAZPDU010000372         1392 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZPDU010000372 DAZPDU010000000
VERSION     DAZPDU010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.376"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..459
                     /locus_tag="ACHURS_004667"
     CDS             <1..459
                     /locus_tag="ACHURS_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR1240196.1"
                     /translation="LNAMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEAT
                     DYYKTPRGQQPGGATKMLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRD
                     GLEVYGRAMVSQDRQLVSLPDFSHYELYDKPEAVQEALEKVIPFFNTHLG"
     gene            477..1115
                     /locus_tag="ACHURS_004668"
     CDS             477..1115
                     /locus_tag="ACHURS_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR1240197.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPVTDGDKPLFM"
BASE COUNT          444 a          282 c          307 g          359 t
ORIGIN      
        1 ttgaatgcaa tggcgtcgca acgaaccaaa gaggctcgtg gtggagaatt acagatcaac
       61 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      121 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa
      181 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      241 ttactgactc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      301 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttca
      361 ttacccgatt tctcacatta tgaactgtat gacaaaccgg aagcagttca ggaggcgctg
      421 gaaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      481 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      541 cagataaaca aaccattaaa caaactttat ttgcccgtca gccagcaaaa atccacaaac
      601 cctatccaac taatagcaag attattatgg gagatgtact gaatcatgca gcactgaagc
      661 aggccatgca gggacaggac gttgtgtatg ccaaccttac gggggaagat ttagatattc
      721 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      781 tttccctcgg tatttatgat gaagttccag gaaaatttgg cgaatggaat aatgcagtta
      841 ttggtgagcc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
      901 aatatacaat tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta
      961 cctcacgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1021 tcacagatat catcgataaa cctgagaaac acattggtga gaatataggt atcaatcaac
     1081 ccgtgactga tggtgacaag ccattattca tgtaaaccaa ttaaatagtt accatcgtaa
     1141 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tccgatgcga
     1201 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacttaca
     1261 tctaagtcag cataattcta atatctgcac atcaataaaa atgcaaacaa cttccggcaa
     1321 aaaaagtgat acaagatcga tgacatagcg ttgaagaaaa ggatttgata atccgcaaaa
     1381 tttgcacaac ac
//
LOCUS       DAZPDU010000373         1384 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZPDU010000373 DAZPDU010000000
VERSION     DAZPDU010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.377"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..1339)
                     /locus_tag="ACHURS_004669"
     CDS             complement(<1..1339)
                     /locus_tag="ACHURS_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFR1240198.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQAR
                     QRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTH
                     AGNPALYPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGY
                     YSLGLLNAWSLAGEHRHWMIPLRKGAQYEELRKLGKGDHLVKLKTSPQARKKWPGLGN
                     EVTARLLTVTRKGKVCHLLTSMTDAMRFPGGEMADLYSHRWEIELGYREIKQTMQLSR
                     LTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLM
                     TLQGASPGRIPELMRDLASMGQLVKLPTRRERAFPRVVKERPWKYPTAPKKSQS"
BASE COUNT          281 a          391 c          357 g          355 t
ORIGIN      
        1 ctgactggct ctttttcggg gctgtggggt atttccaggg cctctccttt accactctcg
       61 ggaaggccct ttcccttctc gtcggtaatt tcacaagttg tcccatactt gcaagatcgc
      121 gcatcagctc cggtatacgt cccggtgaag cgccctgcaa tgtcatcagc attctcatca
      181 ccattccgca tgattctgag aaactcagtt gattcggcca gtaacctttc agatgttccg
      241 ccattttaat catctgatat ctcaccagat tataagccag taagacaccc cacagctctt
      301 gctccacaag ctccggcttt ttacttctca gcgtcagcct gctcagttgc atcgtctgtt
      361 ttatctccct gtatcccagt tcgatttccc agcgatgact gtacagatcc gccatttctc
      421 ctccggggaa gcgcatggcg tccgtcatcg acgtcagcag atggcagact tttcctttgc
      481 gcgtcacggt cagcaggcgg gctgtcactt catttcccag tcccggccac ttttttcgtg
      541 cctgcgggct ggttttcagc ttcaccagat gatcgccttt acccagtttt ctgagctctt
      601 catattgcgc tccctttctg agaggtatca tccagtggcg gtgttctccc gccaggctcc
      661 aggcatttaa cagtcccagt gagtaataac ctttatccat taacgtcaga gtgttatcgc
      721 cggtttgttc tataagttgc tcagcaagct cattttcgct gttcttcatc gtgccgaagg
      781 ctgcagccgt cagcagatgg ctggtcagtt ccatctggca gaccattttg acctgcgggt
      841 agagcgccgg gttcccggca tgtgtctggc gggggaaggc tgcatcgttc tctggtgtat
      901 ccggtgtgcg ccagaacaca ccatcgatgg ccagcagggt caggccgcac cagtgcggat
      961 gcggcgtggc gttatgccag agctgcgctg ttttcgtgaa cacgcggcgg acagcctcac
     1021 ttcccaggcg ctggcgggcc tgaataacgg cactgggggc aacgaagggg cgattgcccg
     1081 gcagcatgat gtccaggcga ttcacaatct ggtgaagagg ttctttacgc tcaagcgcca
     1141 tgccaacaat acaccagacc atcatttcga ggggaagacg gcgcttgcgt agcgttacag
     1201 tacctgattc ggcaaggcaa cgagagatga gttcggggtc gaggtaatcc cccagagaag
     1261 tcagtgggtt acgcagagaa tcgtaacggg ataccagatc aagagcctgt ccaatgtgca
     1321 taaaaaaatc cggaaacaag tgagcgtttc cggattctta cacagccact ggatcggtca
     1381 actg
//
LOCUS       DAZPDU010000374         1380 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZPDU010000374 DAZPDU010000000
VERSION     DAZPDU010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.378"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..1249
                     /locus_tag="ACHURS_004670"
     CDS             <1..1249
                     /locus_tag="ACHURS_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HFR1240199.1"
                     /translation="YRNQLAFIDIFFRGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVL
                     DSGGFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMA
                     SPNGNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAGSPTIRSRLD
                     EMIVLLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYI
                     ENRMYRTPRNLKKLNVIDEGWRLLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVD
                     FDSDKASSAARAAWGNSSYKIILKQSAKEFAKYNQLYPDQFQPLQRDMIGKFGAAKDQ
                     WFSSFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGLSIHEAVWQLAWK
                     KSGPEMASLEAWLEEHEKYRSVA"
     gene            1246..>1380
                     /locus_tag="ACHURS_004671"
     CDS             1246..>1380
                     /locus_tag="ACHURS_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbI"
                     /protein_id="HFR1240200.1"
                     /translation="MTTTQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     P"
BASE COUNT          323 a          355 c          405 g          297 t
ORIGIN      
        1 ctaccgtaac cagctggcgt ttatcgatat tttcttccgg ggaatgaata acaccaacta
       61 caacatggcg gtctgtggca cctccggggc cggtaaaacc gggctgatac agccacttat
      121 ccgcagcgtg ctggactccg ggggctttgc cgtggtgttc gacatggggg atggctacaa
      181 gtccctgtgt gagaacatgg ggggcgtata tctggacggt gaaaccctgc gttttaaccc
      241 gtttgcgaac atcaccgata tcgaccagtc agcggagcgt gtccgtgacc agttgtcagt
      301 gatggccagc cccaacggta acctggatga agtgcatgaa ggtctgctgc tgcaggcggt
      361 cagggcctcc tggctggcca aagagaacag ggcacgtatt gatgacgtgg tggatttcct
      421 gaaaaacgcc agtgacagcg agcagtatgc cgggtcaccg actatccgca gccgtctgga
      481 cgaaatgatt gtgctgcttg accagtacac tgccaacggc acttacggcc agtattttaa
      541 ctctgatgag ccgtccctgc gggatgacgc aaaaatggtg gtgctggagc tgggcggact
      601 ggaagaccgt ccgtcactgc tggttgcggt gatgttctcc ctgattatct acatcgagaa
      661 caggatgtac cgcacgccgc gtaacctcaa gaaactgaac gtcattgatg aaggctggcg
      721 tctgctggac ttcaaaaatc acaaggtcgg tgagtttatt gagaaaggtt accgtaccgc
      781 ccgtcgtcat accggtgctt atatcaccat cacacagaac atcgtcgact ttgactctga
      841 caaggcctcc agtgcggccc gtgcggcatg gggtaactcc tcctacaaaa ttatcctcaa
      901 acagagtgcg aaggagttcg caaaatacaa ccagctgtat ccggaccagt tccagccact
      961 gcagcgcgac atgattggta agtttggtgc ggccaaagac cagtggttca gttccttcct
     1021 gctgcaggtg gaaaaccatt cctcctggca ccgcctgttt gtggacccgt taagccgcgc
     1081 catgtacagc tctgacggcc cggattttga gtttgtgcag cagaaacgta aagaggggct
     1141 gagtattcat gaggcggtgt ggcagctggc gtggaagaag tcagggccgg agatggcttc
     1201 gctggaagcc tggctggaag aacatgagaa atacaggagt gttgcatgac cacaacgcaa
     1261 aaaacgactg atgttacagc cccccgacgc agccactggt ggtggacggt gccgggatgc
     1321 ctggcaatgg tgttactgaa cgcagccgtc agttacggca ttgtcagact gaacgcaccg
//
LOCUS       DAZPDU010000375         1377 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZPDU010000375 DAZPDU010000000
VERSION     DAZPDU010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.379"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..984
                     /locus_tag="ACHURS_004672"
     CDS             <1..984
                     /locus_tag="ACHURS_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR1240201.1"
                     /translation="LVVGVPVPDNIHPMIKDEAGVWSWRTPVLKGNLYEYFFNVDGVR
                     SIDIGTAMTKPQRQVNSSMVLVPGSYLDTRSVAHGDLIAITYHSNALQSERQMYVWTP
                     PGYTGMGEPLPVLYFYHSFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPDTETD
                     AKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALAGLSQ
                     GGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTVVVGD
                     KDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            999..>1377
                     /locus_tag="ACHURS_004673"
     CDS             999..>1377
                     /locus_tag="ACHURS_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFR1240202.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGL"
BASE COUNT          360 a          315 c          362 g          340 t
ORIGIN      
        1 ttagtggtgg gtgttccggt tccggacaat attcacccga tgatcaaaga cgaagcagga
       61 gtctggtcgt ggcgcacgcc tgtcctgaaa ggcaacctgt atgagtattt tttcaatgtt
      121 gatggtgtac gcagcattga tataggcacc gcaatgacta agcctcagcg ccaggttaac
      181 tccagtatgg ttctggtgcc aggcagttat ctggatacgc gttctgttgc gcatggtgat
      241 ttgatcgcca taacttacca ctccaacgca ttgcaatctg aacgtcagat gtatgtctgg
      301 accccgccag gatacaccgg catgggcgag cctttgccag tgctctattt ctatcacagt
      361 tttggtgata ccggacgttc cgctatcgat caggggcgta tcccgcaaat catggataac
      421 ctgctggctg aagggaaaat taaaccgatg ctggtggtga tcccggatac cgaaaccgat
      481 gcgaagggca ttattcccga agatttcgtg cctcaggaaa gacgtaaagt cttttatccg
      541 ctgaatgcta aagcggcaga tcgcgaactg atgaacgata ttatcccgct gattagcaag
      601 cgttttaatg tccgtaaaga tgccgatggt cgcgcgctgg cagggctttc acaaggcggg
      661 taccaggcgc tggtttccgg aatgaatcat ctggaaagct ttggctggct ggccacattc
      721 agtggtgtta ccacgacaac cgtaccggat gaaggtgtcg cggcccggct gaacgatccg
      781 gcagctatta accagcaact acgtaatttt actgtggtcg ttggtgataa agatgtcgta
      841 accggcaagg atatcgccgg gctgaaaact gagcttgagc agaaaaaaat taagtttgat
      901 taccaggaat atcccggcct gaaccatgaa atggatgtct ggcggcctgc ctatgcagcc
      961 ttcgtacaga aattatttaa ataaaataag gcattaagat gaaattaatc attaccgaag
     1021 attaccagga aatgagccgt gtcgcggcac accatctgct tggttatatg tcgaagacgc
     1081 gtcgtgttaa cctggcaatt accgccggta gcacgcccaa aggcatgtat gaatacctca
     1141 ccaccctggt taaaggtaag ccctggtacg ataactgcta tttctataat ttcgatgaaa
     1201 ttccctttcg cggcaaagag ggagaaggcg taacgattac caatctgcgt aatctgtttt
     1261 tcacccctgc ggggatcaaa gaagagaata tccagaagct cactattgat aactaccgcg
     1321 agcatgatca gaaactggcg cgtgaaggcg gcctggattt ggtggtgctg ggattag
//
LOCUS       DAZPDU010000376         1365 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZPDU010000376 DAZPDU010000000
VERSION     DAZPDU010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.380"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="ACHURS_004674"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="ACHURS_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFR1240203.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
BASE COUNT          406 a          253 c          277 g          429 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attgccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacatttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aaggaaattt ttacggcact gaccgttctg cggaaggaat aagattatag
     1201 aattttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt actggaaata agatcagcca ttttt
//
LOCUS       DAZPDU010000377         1365 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZPDU010000377 DAZPDU010000000
VERSION     DAZPDU010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.381"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..1261)
                     /locus_tag="ACHURS_004675"
     CDS             complement(<1..1261)
                     /locus_tag="ACHURS_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFR1240204.1"
                     /translation="MQNRKWILSSLVMTFFGIPILTQFLAAVVAMLGVGLAGIIEVCN
                     ILITPTSYLLLNIFMLALGALMLFFSGRVWAGDSAPEKREIAVWRQCLFLVPGLLILV
                     GWIIALHLADYQFHQMGSGWLADLMLPWLGVLLVSVVGGEYWWIVIIPVGAHISFSLG
                     YGRPTRHPLTGTSGLRCRNSLLFILLMLGFVAGYQGYLYKQLNPGVGVRENIDTWAWR
                     PDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVIPEDFHTREYLES
                     SRTPDAYNRIVKGDADIIFVAQPSGGQKKRAEESGVTLLYTPFAREAFVFIVNADNPV
                     NSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISPQ
                     ETEVASMMEGMIKVVAEYRNTNNAIGYT"
BASE COUNT          380 a          348 c          305 g          332 t
ORIGIN      
        1 aggtgtaacc tattgcgttg tttgtattac ggtattcggc aacgacttta atcattccct
       61 ccatcatact tgccacttct gtttcctgcg gcgagatcat gcggacattt ttcatgacct
      121 gggattgcat caccgtctgg ctaccagagt cttccgggcg ttgccaggtc tggatctcct
      181 gatcgttacc gccaacagta cgccagttgg taattgcacc actgaagatg tcacgcacct
      241 gttgttcagt cagggaatta accggattat ccgcattgac gatgaaaaca aacgcttcac
      301 gggcaaaagg cgtgtacagc aaagtaacgc ccgattcctc agcgcgtttt ttctgcccac
      361 cggaaggttg cgccacaaaa ataatatcag catcaccttt aacaatcctg ttatacgcat
      421 cgggggtacg agagctctcc agatattccc tggtgtgaaa atcttccggt ataacgctta
      481 aagcataaaa tgcagaggca taaattgggt acgccgccgt agcaccatcg agtcgcggcc
      541 agttttgcgt aaactgaatt tgcggtttac cacgcagtgg tgtcagttga ttattgagtt
      601 tatcgggtcg ccaggcccag gtatcaatat tttcgcgcac accgacaccg ggattgagct
      661 gtttatataa ataaccctga taaccagcga caaaaccaag catgagaagg ataaacagga
      721 gggaattacg gcaacgtagc ccggatgtac cggttaaagg atgtctggtt ggccggccgt
      781 accccagcga aaaactgata tgcgcgccaa cgggtataat gactatccac cagtactcac
      841 ctccgactac tgagactaac aaaacgccca gccaggggag cataagatca gccaaccaac
      901 ccgatcccat ctggtgaaat tgataatctg ccagatgcag cgcgattatc cagccaacca
      961 ggattaataa accgggtact aaaaaaagac attgtcgcca gacggctatt tctctttttt
     1021 ctggtgcact atcgcctgcc catactcgcc ccgagaaaaa tagcattaat gcgcccagcg
     1081 ccagcataaa aatgttgagc agaaggtaac ttgttggcgt gataaggata ttacaaactt
     1141 caataatacc ggcaagtccg acacccagca tagcaacaac cgccgccaaa aattgcgtca
     1201 gtatggggat gccgaaaaaa gtcattacca gcgaggacaa aatccatttt ctgttttgca
     1261 ttattctttc cattcttttt gaatggtgaa attatactcc ccgagtcccc ttgccccttc
     1321 tggacacttt tccgaaatga tggcggaaaa aaacgggacc ctttg
//
LOCUS       DAZPDU010000378         1363 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZPDU010000378 DAZPDU010000000
VERSION     DAZPDU010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.382"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..>1363
                     /locus_tag="ACHURS_004676"
     CDS             <1..>1363
                     /locus_tag="ACHURS_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFR1240205.1"
                     /translation="PGTLNDFLGAMTEDDARPEALRRFELMVEEVARNASAVAQNTAA
                     AKKSASDASTSAREAATHATDAADSARAASTSAGQAASSAQSASSSAGTASTKATEAS
                     KSAAAAESSKSAAATSAGAAKTLETNAAASQQSAATSASTATTKASEAATSARDASAS
                     KEAAKSSETNASSSASSAASSATAAANSAKAAKTSETNARSSETAAGQSASAAAGSKT
                     AAASSASAASTSAGQASASATAAGKSAESAASSASTATTKAGEATEQATAAARSASAA
                     KTSETNAKASETRAESSKTAAASSASSAASSASSASASKDEATRQASAAKGSATTAST
                     KATEAAGSATAAAQSKSTAESAATRAETAAKRAEDIASAVALEDASTTKKGIVQLSSA
                     TNSTSESLAATPKAVKAAYDLANGKYTAQDATTAQKGIIQLSSATNSTSETLAATPKA
                     VKAA"
BASE COUNT          364 a          412 c          413 g          174 t
ORIGIN      
        1 acccggtacg ctgaatgatt ttctcggtgc catgacggag gatgatgccc gtccggaggc
       61 actgcgccgt tttgagctga tggtggaaga ggtggcgcgt aacgcgtccg cggtggcaca
      121 gaacacggca gccgcgaaga aatcagccag cgatgccagc acatcagccc gtgaggcggc
      181 aacccatgcg actgatgctg cagattcagc acgtgcagcc agcacgtcag ccggacaggc
      241 cgcttcgtcg gctcagtcag cgtcttccag cgcaggaacg gcatcaacaa aggccactga
      301 agcctcaaaa agtgctgccg ctgcagagtc ctcaaaaagc gcggcggcta ccagtgccgg
      361 tgcggcgaaa acgttagaaa cgaatgcggc agcgtcacaa caatcagcag ccacttctgc
      421 atccaccgcg accacgaaag cgtcagaagc tgccacctca gcccgggatg cgtcggcttc
      481 aaaagaggcg gcaaaatcat cagaaacgaa cgcatcatca agcgccagta gcgcagcttc
      541 ctcggcgacg gcggctgcaa attctgcgaa ggcggccaaa acgtccgaga cgaacgccag
      601 gtcttctgaa acggcagcgg gacagagtgc ctcagctgcg gcaggctcaa aaacagcggc
      661 tgcgtcgtct gccagcgccg cgtcaacaag tgccgggcag gcctcagcca gtgccaccgc
      721 cgccggaaaa tcggcagaaa gcgccgcatc atccgcttca acagccacaa cgaaggctgg
      781 cgaagccact gagcaggcca cggcagcagc gaggtcagct tccgcagcga agacatccga
      841 aacgaacgcg aaagcgtcgg aaacccgtgc agaatcctca aaaacggctg ccgcatcgtc
      901 cgccagttcg gcggcgtcat cggcatcatc ggcgtctgct tcaaaagatg aggcgaccag
      961 acaggcgtca gcagcgaagg gcagcgccac gacggcatcc acgaaggcga cagaggctgc
     1021 tggtagtgcg acggcagcag ctcagagcaa aagtacggcg gaatctgcag caacgcgcgc
     1081 tgagacagcg gcaaaacggg cagaggatat tgcatccgcc gtggcgcttg aggatgcgag
     1141 cacgacgaaa aaggggatag tacagctcag tagtgcgact aacagcactt ccgagtcact
     1201 ggcggcaaca ccaaaagccg ttaaggccgc gtatgacctg gctaacggga aatacaccgc
     1261 acaggatgca acgacagcac agaaagggat aatccagcta agcagcgcga ccaacagcac
     1321 gtctgaaacg ctggcggcaa cgccaaaggc agtaaaagca gcc
//
LOCUS       DAZPDU010000379         1352 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZPDU010000379 DAZPDU010000000
VERSION     DAZPDU010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.383"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..193
                     /locus_tag="ACHURS_004677"
     CDS             <1..193
                     /locus_tag="ACHURS_004677"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240206.1"
                     /translation="RHVKSCCFFSTPPWTNFYSKKTRFVTACAQDISQQSAMGKKNDC
                     LVIFDSGNGDLKENKIFFA"
     gene            393..848
                     /gene="csgB"
                     /locus_tag="ACHURS_004678"
     CDS             393..848
                     /gene="csgB"
                     /locus_tag="ACHURS_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFR1240207.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSTQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            889..1341
                     /gene="csgA"
                     /locus_tag="ACHURS_004679"
     CDS             889..1341
                     /gene="csgA"
                     /locus_tag="ACHURS_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFR1240208.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGNHGGGGNNSGPNSEL
                     NIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSATL
                     DQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
BASE COUNT          403 a          265 c          322 g          362 t
ORIGIN      
        1 tcgtcatgta aaaagttgtt gttttttctc cacacctccg tggacaaatt tttactccaa
       61 aaagacgagg tttgtcacgg cttgtgcgca agacatatcg cagcaatcag cgatgggcaa
      121 gaagaatgac tgtctggtga tttttgatag cggaaacgga gatttaaaag aaaacaaaat
      181 attttttgcg tagataacag cgtatttacg tggcttttaa tactttggta tgaacaaaaa
      241 aagaaaaata caacgcgcgg gtgagttatt aaaaatattt ccgcagacat actttccatc
      301 gtaacgcagc gttaacaaaa tacaggttgc gttaacaacc aagttgaaat gatttaattt
      361 cttaaatgta cgaccaggtc cagggtgaca acatgaaaaa caaattgtta tttatgatgt
      421 taacaatact gggtgcgcct gggattgcag ccgcagcagg ttatgattta gctaattcag
      481 aatataactt cgcggtaaat gaattgagta agtcttcatt taatcaggca gccataattg
      541 gtcaagctgg gactaataat agtactcagt tacggcaggg aggctcaaaa cttttggcgg
      601 ttgttgcgca agaaggtagt agcaaccggg caaagattga ccagacagga gattataacc
      661 ttgcatatat tgatcaggcg ggcagtgcca acgatgccag tatttcgcaa ggtgcttatg
      721 gtaatactgc gatgattatc cagaaaggtt ctggtaataa agcaaatatt acacagtatg
      781 gtactcaaaa aacggcaatt gtagtgcaga gacagtcgca aatggctatt cgcgtgacac
      841 aacgttaatt tccattcgac ttttaaatca atccgatggg ggttttacat gaaactttta
      901 aaagtagcag caattgcagc aatcgtattc tccggtagcg ctctggcagg tgttgttcct
      961 cagtacggcg gcggtaacca tggtggtggc ggtaataata gcggcccaaa ttctgagctg
     1021 aacatttacc agtacggtgg cggtaactct gcacttgctc tgcaaactga tgcccgtaac
     1081 tctgacttga ctattaccca gcatggcggc ggtaatggtg cagatgttgg tcagggctcg
     1141 gatgacagct caatcgatct gacccaacgt ggcttcggta acagcgctac tcttgatcag
     1201 tggaacggca aaaattctga aatgacggtt aaacagttcg gtggtggcaa cggtgctgca
     1261 gttgaccaga ctgcatctaa ctcctccgtc aacgtgactc aggttggctt tggtaacaac
     1321 gcgaccgctc atcagtacta atacatcatt tg
//
LOCUS       DAZPDU010000380         1351 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZPDU010000380 DAZPDU010000000
VERSION     DAZPDU010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.384"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            320..838
                     /locus_tag="ACHURS_004680"
     CDS             320..838
                     /locus_tag="ACHURS_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFR1240209.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTTPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            1048..>1351
                     /locus_tag="ACHURS_004681"
     CDS             1048..>1351
                     /locus_tag="ACHURS_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFR1240210.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLELSLVSQQ
                     FLSLEFPQVLDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWR"
BASE COUNT          346 a          321 c          338 g          346 t
ORIGIN      
        1 aaaaattatc aaaatgataa ttcagaaagg ataaatacaa tatttattca tgattcagct
       61 ttggccattt tatggaggca atacggactt tgaacagttg ggtatggtta tccagatata
      121 tattattaag ttattgctga ataagagatt taatgatttt aaaaatagtt tttacgacaa
      181 ccgtaaattc tagctcggtt tgtatgtaaa aaatactgaa aaatgatgcg gttccgtgaa
      241 gtgagctgca tctgcctgtc tgggattaac ggcaggtcat tgtaagtagt aattaaccac
      301 gttaacaagg agtttacaaa tgccaacacc gtgttatatc tccatcactg gtcagaccca
      361 gggaaatatt actgcaggtg catttaccgc tgattctgtc ggcaacatct atgtgcaggg
      421 gcacgaagac gagatgctgg tgcaggaatt cctgcataac gttactgtcc cgaccactcc
      481 tcagtccggt cagcctgcgg gtcagcgtgc tcacaagcct ttcattttta ccgtggcgct
      541 gaacaaagcg gttccgctga tgtacaacgc actggcctcc ggcgaaatgc tgccaaccac
      601 tgaacttcac tggtggcgta cctctgtgga gggcaaacag gagcattatt tcactacccg
      661 cctgactgat tcgacgattg tcgatatgaa actgcatatg ccgcattgcc aggacccggc
      721 gaaacgcgag ttcacccagt tgctcgaggt ttcactggcc taccgcaaaa ttgaatggga
      781 acacgtcaaa tctggcacct ccggtgccga cgactggcgc gcaccactgg aagcataagt
      841 taagccaaca gcatccggct atcaggccgg atgctgtttt tgtattacgc cgtagaaaga
      901 aaggttgtca gtaaggggtt acgttcacgg gagatggcgg atgccggaaa cttcggcggt
      961 agcgtgcctg aacgcaagcg taacactgta ctgtcacagc cgctttaaca gcgaactgtc
     1021 tgtttttcct taaccccgga ggttgctatg tcaaccggat tacgtttcac gctggaagtg
     1081 gacggcctgc caccggatgc ttttgcggtg gtctcctttc atctgaacca gtcactctct
     1141 tcgctctttt ccctcgagct ctctctggtc agccagcagt ttctctccct tgaatttccg
     1201 caggtactgg acaaaatggc ctacctgacg atatggcagg gcgatgacgt acagcgccgg
     1261 gtgaaaggtg tggtgacctg gtttgaactg ggggagaacg acaaaaacca gatgctgtac
     1321 agcatgaagg tatgcccacc gctgtggcgc a
//
LOCUS       DAZPDU010000381         1341 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZPDU010000381 DAZPDU010000000
VERSION     DAZPDU010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.385"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..184
                     /locus_tag="ACHURS_004682"
     CDS             <1..184
                     /locus_tag="ACHURS_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240211.1"
                     /translation="TISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQ
                     GKRRCQQQFTSQEVQN"
     gene            194..>1341
                     /gene="yicI"
                     /locus_tag="ACHURS_004683"
     CDS             194..>1341
                     /gene="yicI"
                     /locus_tag="ACHURS_004683"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFR1240212.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPG"
BASE COUNT          335 a          318 c          351 g          337 t
ORIGIN      
        1 cacgattagc atcattattg cgctattcac gattgttccg gcgatctgtt atttgctgag
       61 cgcgattatc gctaaacgct actactcact cacgacgcac aatctgaaaa ccgttatgga
      121 acagctggct cagggtaaac gccgttgcca gcaacaattc acctctcaag aagtgcagaa
      181 ctaaggaacg gcaatgaaaa ttagcgatgg aaactggttg attcaacctg gcctcaattt
      241 gattcacccg cttcaggtgt tcgaggttga acagcaggat aatgaaatgg tggtctatgc
      301 tgccccccgt gatgtgcgtg aacgtacctg gcagcttgat acgcctttat ttacgctgcg
      361 ctttttctcc ccacaggaag gtattgtcgg tgtgcggatt gagcattttc agggggcgct
      421 gaataacggt cctcattatc cgctcaatat tttgcaggac gtgaaggtca caatcgaaaa
      481 cacagaacgt tatgctgagt ttaaaagtgg caacttaagc gcgcgtgtca gcaaaggtga
      541 gttctggtca ctggattttc tgcgcaacgg cgaacgtatt accggtagtc aggtgaaaaa
      601 taatggctac gtgcaggaca cgaataatca acgcaattat atgtttgagc ggcttgatct
      661 tggcgttggc gaaacagttt acggtctggg agagcgcttt actgccctgg tgcgcaatgg
      721 ccagacggta gagacctgga accgggacgg cggcacaagt actgaacagg cgtataaaaa
      781 tatcccgttc tacatgacta accgtggtta tggggtactg gtcaatcatc cccagtgtgt
      841 ctcttttgaa gtgggatcgg agaaagtctc caaagtgcag ttcagcgttg agagtgaata
      901 tctcgaatac tttgttatcg acggcccgac gccgaaagcg gtacttgatc gttatacccg
      961 ctttactggt cgtccggcgc tgccgcccgc gtggtccttc ggcctgtggc taaccacttc
     1021 atttaccacc aactacgacg aagcgacggt aaacagcttt atcgatggta tggcggaacg
     1081 caatctgccg ctgcatgttt tccactttga ctgtttctgg atgaaagcct tccagtggtg
     1141 cgattttgag tgggacccgc tgactttccc tgacccggaa gggatgatcc gccgcctgaa
     1201 agcgaaaggg ctgaaaatct gcgtctggat taacccctat atcggtcaaa aatcccccgt
     1261 ctttaaagag ttacaagaga aaggctattt actcaaacgc ccggacggtt cgctatggca
     1321 gtgggataaa tggcagccag g
//
LOCUS       DAZPDU010000382         1320 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZPDU010000382 DAZPDU010000000
VERSION     DAZPDU010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.386"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..346)
                     /locus_tag="ACHURS_004684"
     CDS             complement(<1..346)
                     /locus_tag="ACHURS_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240213.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERA"
     gene            complement(349..1059)
                     /locus_tag="ACHURS_004685"
     CDS             complement(349..1059)
                     /locus_tag="ACHURS_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240214.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(1075..>1320)
                     /locus_tag="ACHURS_004686"
     CDS             complement(1075..>1320)
                     /locus_tag="ACHURS_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR1240215.1"
                     /translation="FRYYATQMNADKNIKLLAINGIAPTAENIRNGKYPYIVDAFMVT
                     RENTTSETQKLLEWFLTPQGQSLVEDVGYVPMYKTLP"
BASE COUNT          351 a          317 c          293 g          359 t
ORIGIN      
        1 ttgcccgttc atccgcacgc cagttttctt tgttggcaag gttgatacgg cgcagagaaa
       61 aaactacgtg aatattgtca aaatgcccct gtgcaacaaa ttccagtaaa cgcggcagaa
      121 aatagcggac ctcatcgcta ttttgcggat caggccctgg gctactattc caggcattga
      181 ttaaggaata cgggatcgct cgtaacgatg tactccgtaa tgccttttgt tcctgctgac
      241 tcaggcaata ttcacaacaa accacaaatt tctgcggcac ccgataacga cgaaaggtgt
      301 gataaatctg ctcaattaat gcctgcatgg cggggctggg ttgcatagtt accccctcgc
      361 ccgctgatcg cgtgtaaacg gttgatagac aaaacgagca ttcgccgcgt tcaggcgaat
      421 aagggcgcta cgcgaaaggg aaaagctggg gcgaatgcaa ctattgcagt ctcgacagct
      481 ttcctctgcg caattttcca ctgagtaatg acgcgtgtag agtttcagtt cggcctgctt
      541 atcgcgaaat ccgcgtaaac tgttattgtt atgaaatttc caggcatctt taaagacctg
      601 aaagatatag acatcctcaa tccaggagtc acatttttta agcgggcgct ttgctattat
      661 cacccgctca tccggattta gcatcgccgt aaattgtacg gtgacgtcat ttgtcgtgtt
      721 caggtcacat tgaacatctg gtacatcact gatatagggg tcaaatccaa aatcagcacc
      781 acaggcacaa agacggctaa tggtggcggt atcgagggaa aatcccggcc ataccttgct
      841 ggaggttaag ccaatattta atgtgacatc atatcgaagc aatccctcaa gaccgtcagc
      901 accgacgaac ttttcccctt catccaaggc ctggttaata agttgctgaa caatgccttc
      961 atcccagtat gccgatttgc agttggcaat cgtaatatcc acgcctgtga agggatcagt
     1021 accggcaagg cttgcaaata atgtattaac taattccatt tttattatcc agctttaagg
     1081 tagcgtctta tacatcggga cataacctac atcttccacc aaactctgtc cctgcggcgt
     1141 taaaaaccac tccaacaatt tttgtgtctc tgacgtcgta ttttcccgcg ttaccataaa
     1201 ggcatcgacg atatagggat atttgccgtt gcgaatgttt tcggcagtcg gtgcaatacc
     1261 gttaatcgct agcagtttta tatttttatc ggcgttcatt tgtgttgcgt aataacggaa
//
LOCUS       DAZPDU010000383         1299 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZPDU010000383 DAZPDU010000000
VERSION     DAZPDU010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.387"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..1117
                     /locus_tag="ACHURS_004687"
     CDS             <1..1117
                     /locus_tag="ACHURS_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR1240216.1"
                     /translation="TWYGWDGDRLTTVQTQQTRIQTVYQPGSFTPLLRIETENGEQAK
                     ARHRSLAEVLQEDTGVTLPAELAVMLGRLERELRAGAVSAESEAWLAQCGLTVEQMES
                     QMEAEYIPERRLHLYHCDHRGLPQALISPEGETAWRGEYDEWGNQLNEENPHHLYQPY
                     RLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGINLYTYPLAPIRYTDPLGL
                     ERVISVYGPPAPDRAGAETPLVLTDMTGGVTIYYDPETGDSMTFDSSNRIDRRSQRGA
                     GDPYTGEVVGCETNGSGISAAYGTTKIYTTDTRARWLHGGGSSLRDPYAPRQGWKPTM
                     GCTRAQNEDVDELCKKVTSWMYSHPGERIRYERFKTR"
     gene            1095..>1299
                     /locus_tag="ACHURS_004688"
     CDS             1095..>1299
                     /locus_tag="ACHURS_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240217.1"
                     /translation="MNVLRHVNAINMGVCILILVIGCANAKVLKRCILPAPDGGVEKL
                     SKPNLHGVIKKVDLNTNEAEIKIK"
BASE COUNT          356 a          263 c          400 g          280 t
ORIGIN      
        1 gacctggtac gggtgggacg gtgacaggct gaccactgta cagacccaac agacaagaat
       61 ccagacggta taccagccgg gaagcttcac gccgctcctg agaatcgaaa cagagaatgg
      121 cgaacaggcg aaggcgcggc accggagcct ggcggaggtg ttgcaggagg acacgggggt
      181 gacgcttccg gcggagctgg cggtgatgct ggggaggctg gagcgggaac tgcgtgcggg
      241 tgcggtgagt gcggagagtg aggcatggct tgcgcagtgc gggctgacgg tggagcagat
      301 ggaaagccag atggaggcgg aatacatccc ggagcggagg ctgcatcttt accactgtga
      361 ccaccgggga ctgccgcagg cgctcatcag cccggaaggg gaaacggcgt ggcgcgggga
      421 gtatgacgag tgggggaacc agcttaatga ggagaacccg catcacctgt accagccgta
      481 ccgtttgccg gggcagcagt atgatgagga gtcgggactg tattacaacc gtcaccggta
      541 ctacgatccg ttgcaggggc ggtatatcac ccaggatccg atagggttga aggggggaat
      601 aaatctatac acgtatccac tggcaccaat acgttacact gatccactgg ggttagaacg
      661 agttataagt gtatatgggc ctcccgctcc agaccgggca ggtgctgaaa ctcctcttgt
      721 tcttactgat atgactggag gagtgactat ttattatgat cctgaaacag gtgattcaat
      781 gacttttgat agtagcaata gaattgatcg tcgttctcaa cgtggtgctg gtgatccata
      841 tacaggtgaa gtcgttggtt gtgagacaaa tggatcagga atatcggcag catatggcac
      901 aacgaagatc tatactacag atactagagc aagatggcta catggtgggg gatcatcgtt
      961 gcgcgatcct tatgctcctc gacaaggctg gaaaccaact atgggatgta cccgtgcaca
     1021 gaatgaagat gtagatgagt tgtgtaagaa agtgacaagt tggatgtatt cgcatcctgg
     1081 agagagaatt agatatgaac gttttaagac acgttaatgc gattaacatg ggagtgtgca
     1141 ttctaatttt ggttattgga tgtgcaaatg ctaaagttct taaacgatgt atattacccg
     1201 ctcctgatgg tggcgtagaa aaactatcaa aacccaactt acatggagta attaaaaaag
     1261 ttgatttgaa cacaaatgaa gcggaaatta aaataaaag
//
LOCUS       DAZPDU010000384         1296 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZPDU010000384 DAZPDU010000000
VERSION     DAZPDU010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.388"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..1165)
                     /locus_tag="ACHURS_004689"
     CDS             complement(<1..1165)
                     /locus_tag="ACHURS_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR1240218.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENMPSANYQFNTFFSNAFETDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQDQLDKHFTIESHPLLYRHT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKGAFIHFYDYSRRYAISENIEKDAVTNNIA
                     LMNTFYDSDNTSGEVLNNDFTEEEPFETALDVEHTIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMRSSHYHDICSVCVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGFFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCC"
BASE COUNT          425 a          244 c          187 g          440 t
ORIGIN      
        1 aacaacattt tttcaatatg ctttcattca caacagtgtg tgataagtag agattactta
       61 tgttttgctt tattaatgcc acgtcattaa aatgactata tttaatgaaa aaaccatcat
      121 tgagtagtgt tttaagattg actaaattac ttgcatcttc acgtaccatc gtggcaactg
      181 gaacaggcag agacatgaaa acttcttcat tcaccatcaa gtgaatcatg cattgcgttc
      241 cctttgtcat atccacacat acgctacaaa tatcgtggta atgcgaagag cgcatttcag
      301 gaaacagatt aaacacggta tcttgaaagg cactaaatcg tgttaataaa tcctgataat
      361 gaacaggttt tgtttcaaaa ttatcatcag caaatccaag cactattgta tgctctacat
      421 ctaacgcagt ttcaaatggc tcttcttctg taaaatcatt atttaaaacc tcacctgacg
      481 tattatcact gtcataaaaa gtgttcatca atgcgatatt atttgttaca gcgtcttttt
      541 cgatattctc actaattgca tatcgcctgc tataatcata aaaatgaata aacgcgccct
      601 ttataaagga tatatattgt tctttgttaa agtcaatacg gctgtataat ttttcaaaaa
      661 tcacattcgc aattttacta ccaatggtat gcctgtataa taatgggtgc gattcaatcg
      721 taaaatgctt gtctaattga tcttgtaatt tattctcata gtccagaggg aaaagaaact
      781 gttgtctttc aagtttccca ttcgatacat tacgtattcc aatagagatt ctttcattac
      841 ccgcctttat ttcaacttcg ttatcttctg atttacccag atatttttta acatcagtct
      901 cgaaagcatt actaaaaaaa gtattaaatt ggtaattcgc tgatggcata ttttccgcga
      961 gcaacacatc attttttatg agatcactgt cagcatttat tgctaatatc agatgcaagt
     1021 agatggtatc ccatgtagac aactcaccct tacgactgga atttaatatc gcgaaaaatt
     1081 ccctcagtga atccaggaaa ttatcacttt gatttagttg tgtgcctgga aatgatgatg
     1141 cttgatcaga tatattgttt atcatattaa aaatcaccca agaaaaacga taatccaccg
     1201 aactcctcgt gctttataat tataaaagca caacgccttt tatattcata ttaattaacg
     1261 agatactgaa acaacttaat ttattcttcg ctaaaa
//
LOCUS       DAZPDU010000385         1288 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZPDU010000385 DAZPDU010000000
VERSION     DAZPDU010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.389"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..100
                     /gene="yjbL"
                     /locus_tag="ACHURS_004690"
     CDS             <1..100
                     /gene="yjbL"
                     /locus_tag="ACHURS_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YjbL"
                     /protein_id="HFR1240219.1"
                     /translation="ALRHRDIIIAKIDLFIQRITHNLWHARKKMCF"
     gene            91..831
                     /locus_tag="ACHURS_004691"
     CDS             91..831
                     /locus_tag="ACHURS_004691"
                     /inference="COORDINATES: protein motif:HMM:NF022352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFR1240220.1"
                     /translation="MFLIEQINDLKMWVNKYIDDCTDEDLNDRDFIASVVDRAIFHFA
                     INSICNPGDNKDATPIEQCTFDVETKNGLPSTVQLFYEESKDNEPLANIHFQAIGSGF
                     LTFVNACQEHDDNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLSQR
                     DMKKYLGEMKRLADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSF
                     DPTSKEEIKILTDTSKLIYDLFGVKSEK"
     gene            1194..>1288
                     /locus_tag="ACHURS_004692"
     CDS             1194..>1288
                     /locus_tag="ACHURS_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HFR1240221.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAP"
BASE COUNT          408 a          213 c          258 g          409 t
ORIGIN      
        1 agcgctgcgt catcgggata taattattgc aaaaatagat ttgtttattc aacgaatcac
       61 gcataattta tggcatgcac gtaaaaaaat gtgtttttaa ttgagcaaat aaatgatctc
      121 aaaatgtggg tcaataagta tattgacgat tgcactgatg aggatttaaa cgatcgtgac
      181 tttattgcat cagttgttga ccgggctatt tttcatttcg cgattaatag tatatgtaat
      241 cctggggata ataaagatgc gactcccatt gaacaatgta cttttgatgt agaaactaag
      301 aatggccttc cctccacggt tcagctattt tatgaggaat ctaaggataa tgaaccttta
      361 gcgaatatac attttcaagc aataggttct ggttttttaa cgtttgttaa tgcctgccag
      421 gaacatgatg acaacagctt aaaattattt gcttcgctgt taatttcact ttcatattct
      481 agtgcctacg cagatttatc agaaacagtg tatattaatg aaaataatga gagctacctg
      541 aaagctcagt ttgaaaaatt atctcaacgt gatatgaaga agtacctggg agagatgaag
      601 cgtctggctg atgggggaga aatgaatttt gatggctatc tggataagat gtcacatctg
      661 gtgaatgaag gaacgctcga tcctgatatt ttaagcaaaa tgcgagatgc tgcaccacaa
      721 ttaattagct tcgcgaagtc gtttgaccca acctcaaagg aagagattaa aatacttaca
      781 gacacttcta aattaattta tgatttgttc ggggttaaat cggagaaata atatgtgaag
      841 ttcttcgata gtatggaagg cattatataa aaggacccaa tatttattgg gttctttttt
      901 ctctatcaat gctattagca gggagatata tcaccagagt ttaatgtgtg attttttatt
      961 tatcgtcgaa cctggattgt ttatcattgg ccttaacaaa gttaacggct aataagatta
     1021 tttccatcac ttcgtgagag cttcatgcct tgagaggatc tcaattttct tttgcaatga
     1081 gacaggcgct tcctgttgtt atggtatagt accccgctat tgagcctcct gaatagtgat
     1141 gctgaataac ataaccccat gatatatcga taaaataatc tctacatttg aaaatgcacg
     1201 gtaattctga aatgcaaaaa atcaaccaaa ccagcgcaat gcctgaaaaa actgacgttc
     1261 actggagtgg tcggtttagc gttgcacc
//
LOCUS       DAZPDU010000386         1285 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZPDU010000386 DAZPDU010000000
VERSION     DAZPDU010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.390"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            7..498
                     /locus_tag="ACHURS_004693"
     CDS             7..498
                     /locus_tag="ACHURS_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR1240222.1"
                     /translation="MSTETIEIFNNSDEWANQLKHALSKGENLALLHGLTPDILDRIY
                     AYAFDYHEKGNITDAEIYYKFLCIYAFENHEYLKDFASVCQPKKKYQQAYDLYKLSYN
                     YSPYDDYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDDSVKSKAQAYIELLNDNSE
                     DNG"
     gene            833..>1285
                     /gene="ygeH"
                     /locus_tag="ACHURS_004694"
     CDS             833..>1285
                     /gene="ygeH"
                     /locus_tag="ACHURS_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
                     /protein_id="HFR1240223.1"
                     /translation="MDLENKFSYHFLEGLTLTEDGILTQGNEQVYIPQKELGVLIVLL
                     ESAGHVVLKDMIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCIETIYRKGYRFS
                     GQVFKTKINEDNTSDYSIAIFPFTTSLNTLDPLILNQELVQIISNKKID"
BASE COUNT          470 a          197 c          210 g          408 t
ORIGIN      
        1 aatattatga gcacagaaac aattgaaata ttcaataata gtgatgaatg ggcaaatcaa
       61 ctaaaacacg cattatcgaa aggagaaaat ctggcattac tacatggttt aactcctgat
      121 atccttgata gaatatatgc atatgcattc gactaccatg aaaagggtaa tataacggac
      181 gcagaaattt attataaatt tttgtgcatt tatgcgttcg aaaatcatga gtatctaaaa
      241 gattttgcat cagtatgtca gccaaaaaag aaatatcaac aagcatatga cctttacaaa
      301 ctaagttaca attactcccc gtatgatgac tattcagtta tttatcgtat gggtcaatgt
      361 cagattgggg ctaaaaatat cgataacgca atgcaatgtt tctatcacat tattaacaat
      421 tgtgaggatg atagtgttaa gagtaaagcg caggcatata ttgaactctt aaacgataat
      481 tcagaagata atggctaagc attacaatca tttggttaaa tttgctggtt atagtgcagt
      541 taactactat aaccagcacc tcttatgttt tcttaaatca gcatcttaaa acagttaaga
      601 gcatactata aatcattatt tttcaatgtg ttagagaaca atctacttta taaaaattga
      661 ataaggctat ttcagattac atcactgaat tcctaagata aattagtgaa cacaaccata
      721 gatatgtcga atgtattttc ctgtagcatt aatgacacat aaatatgaat agccataatt
      781 tctattgctt aagcaggatg caagaaacca attttttcat agaggttaac taatggactt
      841 agaaaataaa ttctcatatc attttcttga gggattaacg ctcacggaag atggaattct
      901 tactcaagga aatgagcaag tttatattcc acagaaagag ttaggtgtat taatagtatt
      961 acttgaatcc gctggtcatg ttgtactgaa agatatgatc atcgaatcag tatggaaaaa
     1021 tattattgtt agtgacgagt ccctgacaag atgtatctat tctttgcgct gcatttttga
     1081 aaaaattggc tatgatcgtt gcatagaaac aatctaccgg aaaggttatc gtttcagcgg
     1141 gcaggttttc aaaactaaaa ttaatgaaga taatacttca gactattcca tagctatatt
     1201 ccctttcact acttcattga atacactgga tccattaata cttaatcagg aattagtgca
     1261 aatcatttca aataaaaaaa tcgat
//
LOCUS       DAZPDU010000387         1282 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZPDU010000387 DAZPDU010000000
VERSION     DAZPDU010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.391"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            132..1151
                     /locus_tag="ACHURS_004695"
     CDS             132..1151
                     /locus_tag="ACHURS_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc11 family transposase"
                     /protein_id="HFR1240224.1"
                     /translation="MKYTPVGVDIAKHVIQIHFINEHTGEVVDKQLRRQDFLTFFGNR
                     EPCLIGMEACGGSQHWARELTKLGHKVRLLQARFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKEIAVKTEEQQSVLVLHRTRMQLVKFRTAQINALHGTLLEFGETIHKGRAAMEREF
                     PEALERMKERLPPYLIMVLENQYNRLNELDSLIEDIEKQLTSVARQNETCKRLLDIPG
                     VGPLIATAAVATMGEASAFKSGREFAAYVGLVPKQTGSGGKVRLLGISKRGDTYLRTL
                     FIHGARAVALVAKEPGPWITELKKRRPASVAIVAMANKLARTVWAITAHDRKYDRNHV
                     SIRPY"
BASE COUNT          368 a          285 c          357 g          272 t
ORIGIN      
        1 cacacaatta aacacggagc agtatgatgg ttaaaagggt gaaactggca ggtgtggagc
       61 ctcgcagatt tccggcatca tagtgcccaa tacgggatgc ataaagctcc ctcagactgg
      121 agactccaca aatgaaatat acaccggttg gcgttgatat cgcaaaacat gtcattcaga
      181 ttcacttcat caatgagcac acaggtgaag tggttgataa acagttgcgt agacaggatt
      241 ttctgacgtt cttcggcaac cgtgagccat gcctgattgg tatggaggcc tgtggaggtt
      301 ctcagcactg ggcacgggaa ctgacaaaac ttggtcataa agtccggttg ttgcaggccc
      361 gcttcgttaa ggcattcgtc atgggcaata agaatgatgt gatggatgcc cgggctatct
      421 ggatggcggt tcagcagccg ggtaaagaaa tcgccgtaaa aacagaagaa cagcagtcgg
      481 tactggttct gcaccgtacc cgcatgcaac tggtgaagtt ccggaccgca caaattaatg
      541 ccctgcacgg gacgttactg gagtttggtg aaaccatcca caaaggccgg gcagcgatgg
      601 agcgggagtt ccccgaagca ctggaacgga tgaaagagag actgccaccg tatctcatta
      661 tggttctgga aaaccagtac aaccgactga atgagctgga ctcactgata gaggatattg
      721 aaaaacagct taccagcgtg gcgaggcaga atgaaacctg taagcggttg ctggatattc
      781 ctggcgttgg accacttatt gcgacggcag cggtggccac catgggggaa gcatcagcgt
      841 ttaaatcggg gcgagagttc gccgcatatg ttggtctggt tccaaaacaa actggctccg
      901 gagggaaagt acgtctgctg gggataagca aacgtggtga cacttatctc aggacattat
      961 ttatccacgg tgcaagagcg gtggcattag tagctaaaga gcctggcccg tggataaccg
     1021 aactgaaaaa acgtcgtcca gccagtgtgg caatcgtcgc catggcaaac aagctggcac
     1081 gaacagtatg ggcgataacc gcccatgacc gtaagtatga caggaaccac gtcagtatca
     1141 gaccatatta atcgctgata ccattaaaca atgaactctt aacaaaaggg tgaatgctga
     1201 aaggttgcta tggcggccag agtgatgaca aagacaggta agaccgtgac tcactaaacc
     1261 tgaacagtat tttgggcttg aa
//
LOCUS       DAZPDU010000388         1266 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZPDU010000388 DAZPDU010000000
VERSION     DAZPDU010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.392"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            2..1197
                     /gene="bluF"
                     /locus_tag="ACHURS_004696"
                     /pseudo
     CDS             2..1197
                     /gene="bluF"
                     /locus_tag="ACHURS_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
BASE COUNT          365 a          266 c          275 g          360 t
ORIGIN      
        1 aatgcttacc acccttattt atcgtagcca tatacgtgac gacgaacctg tcaaaaaaat
       61 cgaagaaatg gtttcgatag caaatcgcag gaacatgcag tctgacgtaa cagggatctt
      121 actgtttaat ggttctcatt ttttccagct tctggaaggt ccggaagaac aggttaaaat
      181 gatatatcgg gctatatgcc aggatccacg gcactataat attgttgagc tgctgtgcga
      241 ttacgcgcct gctcgccgtt ttggcaaagc gggaatggaa ttatttgatt tgcgcctgca
      301 cgagcgagat gacgttttac aggccgtatt cgacaaaggc acatcaaaat ttcagctaac
      361 ttatgatgac agagcgctac aattttttcg tacttttgtc cttgcaaccg aacaatcaac
      421 ctatttcgag atccctgccg aagactcctg gctttttatc gctgacggat ctgataaaga
      481 acttgattcc tgtacccttt caccaactat aaacgaccac tttgcctttc atcccttatc
      541 gcggcggata atcgcttttg aagccattgt gcaaaaaaat gaagcccatc agccatagcg
      601 gttgggcagc gtaaagacgg ggaaatctac acagcggatc tcaaaagtaa ggcgcttgca
      661 ttcgcgatgg cacacgcact tgagctcggt gataaaatga tttcaatcaa tctattacct
      721 atgaccctgg ttaacgaacc tgacgcagtc tcttttttac ttgatgaaat aaaggccaat
      781 gctctggtgc ctgaacaaat catcgttgaa tttactgaaa gtgaagttat atctcggttt
      841 gatgagtttg cagaagcgat taaatcgctt aaggctgccg gtatcagtgt agcaattgat
      901 cattttggcg caggttttgc cggtttgtta ctcctgtcac gcttccagcc tgacagaatt
      961 aaaatcagtc aggaattgat taccaatgtt cataaaagcg ggccacggca ggcaattatt
     1021 caagcgatca taaaatgctg tacatcactt gaaattcaag tcagtgctat gggcgtggca
     1081 acaccagaag agtggatgtg gcttgaatcc gcaggaattg agatgtttca gggagatctg
     1141 tttgctaaag ctaaattgaa tggtatccct tccatagcgt ggccagagaa aaaataattt
     1201 tcagcacatt ctttcacatg atttcagtaa atcatcgtga aaatgaatac gttatgttta
     1261 aattta
//
LOCUS       DAZPDU010000389         1262 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZPDU010000389 DAZPDU010000000
VERSION     DAZPDU010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.393"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            62..563
                     /gene="tnpA"
                     /locus_tag="ACHURS_004697"
                     /pseudo
     CDS             62..563
                     /gene="tnpA"
                     /locus_tag="ACHURS_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(581..1249)
                     /locus_tag="ACHURS_004698"
                     /pseudo
     CDS             complement(581..1249)
                     /locus_tag="ACHURS_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
BASE COUNT          331 a          289 c          283 g          359 t
ORIGIN      
        1 ctcctgttga ccacctggtc ttaactggaa tttaaatgcc tgtaatcgtt tcacaggtca
       61 aatgatactt tggtctgtga aaaaagaaac cgatatccgg cgtggaaggc attgtgtttt
      121 cctgatgcat gttcacctgg tctttgtcac tagataccga cgccagattt tttgacgcga
      181 cagaaaaact acgcacttac ttttcaaatg tatgtgctta ttttgaagct gaactggttg
      241 aaatggatgg cgaaccagat cacgtccatt tgttaattaa ctaacctccc aaactggcga
      301 tatccagtct ggtaaacagc ctcaaaggcg tatcgggtag gttactgcga cgagatcgac
      361 cagatattgc agtcaggtat tactacaaag gcgttttgtg gagtcctggc tattttgcca
      421 gtagctgcgg aggtgtgcca atatccgtca tccgccaata cattgaacaa cagcaaacac
      481 ctggtcaggt ggaaaaccgc gccttatatc cccgccctga aggatggggt tttacggcgc
      541 accggataaa aagcgccatc taacgatggc gcgtctggaa ctactttttg ttaacgtcaa
      601 acatggtggc atcggtcgcc atgtcatcaa taacttgttt aatattttcg aaagccattg
      661 gcgtactttc gttattcagg tctttacctt cgccctgacg aacgactttg ataaccggtt
      721 tattagtcgc tgcatcaatc agctcacctt caaaatagag gcgagtatcc atggtacggt
      781 ggcctgtagc catttgcgtc cccgcaacca ctaatgcaac aggaaccact tcatagaatt
      841 gcagcccttc tttgctggta tctacaccgg taatggcccc acggaaaatc agactacgcg
      901 gcccagcggt ggtaaccagt ggtttacgct gcgctatcgc ttctttcatt tcggtgttgg
      961 tatagttcaa aattttatcc agaactttct gccctaccta ggtagaaggt tttggcaccg
     1021 gataataagt gattgggttc cagacgatgc tgtcatattt gctttgatca aaactcgggt
     1081 ctacccaacg taaaacaggt ttacctgttg ctgaggttgt ttcttttaaa tcagagtaat
     1141 tgtttaaaaa accagaatat ttatctggct gggtgatttt agaggcacaa ccagacagag
     1201 ccaacaagcc gcataaagct gcaactttcg caaatgatgt ggtacgcatg aatagtttcc
     1261 tg
//
LOCUS       DAZPDU010000390         1239 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZPDU010000390 DAZPDU010000000
VERSION     DAZPDU010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.394"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..443
                     /gene="espX1"
                     /locus_tag="ACHURS_004699"
     CDS             <1..443
                     /gene="espX1"
                     /locus_tag="ACHURS_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HFR1240225.1"
                     /translation="CVLTNISCFESDLSNTIFSNGEINNLFIKKSNIFGTSFTNTMIK
                     NLRCEDIMPGRWTTQLVNKHLGYRYTGVFKTLASIDDKPSRFEILIPLVQTLVRDNVK
                     LNNDVYKELNKFMHDYDKTSSKMRKYLQSINECMLLIKNIVHQD"
     gene            677..1239
                     /locus_tag="ACHURS_004700"
                     /pseudo
     CDS             677..1239
                     /locus_tag="ACHURS_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT          445 a          228 c          208 g          358 t
ORIGIN      
        1 gttgtgtatt aaccaatata tcatgttttg aaagcgactt atcaaacacc atcttttcaa
       61 atggagaaat aaacaacctc ttcattaaaa aaagcaacat atttggaaca tcgttcacta
      121 ataccatgat aaaaaatttg cgctgtgaag atattatgcc tggccgatgg accactcaac
      181 ttgtcaataa gcacttaggt tacagataca caggagtatt caaaaccctg gcaagtattg
      241 atgacaaacc gtcccgtttc gaaattttga taccattggt acaaacttta gttcgcgata
      301 atgtaaagct aaataatgat gtgtataaag aacttaataa gtttatgcac gattacgaca
      361 aaacatcatc aaaaatgaga aaataccttc agagcatcaa cgagtgcatg ctccttataa
      421 aaaatatagt tcatcaggat taaaacactc aaagaaagag gtaattttag aatgaaatta
      481 tctgtgatga ttattaatac actgcagaga aataaaaaac aatctgaagc atgctctgaa
      541 acatcctact ctgtcactct aatgatggca ccttgctctt cttataaaga agggaacaat
      601 taagaatatt aaattttcaa actttcatca acttcacatc aagaaattaa aaaatttaaa
      661 cattaatcta aataataaca acctgatcac ataatcgggt acgattttat ccgataacta
      721 taatgctata actctgggaa atggttggaa tacaccgctg ggtgccatct ctttttacgc
      781 tgcccgatcg agtagtaagt taaataatga taccagacac gaaggtacga gctatcaggt
      841 cgcctataat aaatatctag tacaaacagc cactcgattt agcgtggccg cctggcgtta
      901 tgcttcgcag gattacagga cattcagcga ccatctttac gaaaatgata aacactatca
      961 tcagagtgat tatgatgatt tttatgatat tggcagaaaa aatagccttt ctgccaatat
     1021 tatgcaacct ttatccaata atctgggaaa tgtatcatta agtgcgcttt ggcggaatta
     1081 ctgggggcga agtggaaatg ctaaagatta ccaattcagt tactccaata actggcaaca
     1141 cattagttat actttctctg ccagccaatc ttatgatgaa aataataaag aagaggagcg
     1201 ttttaatctg tttatctcca ttcctttcta ctgggggga
//
LOCUS       DAZPDU010000391         1215 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZPDU010000391 DAZPDU010000000
VERSION     DAZPDU010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.395"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            349..582
                     /locus_tag="ACHURS_004701"
     CDS             349..582
                     /locus_tag="ACHURS_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFR1240226.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALVQKGSGNFSAWVIEA
                     CRRRLCSEKRVSPEANKEKSDITELLRKQVRPD"
     gene            640..1050
                     /locus_tag="ACHURS_004702"
     CDS             640..1050
                     /locus_tag="ACHURS_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HFR1240227.1"
                     /translation="MAQVAIFKQIFDKVRNNLNYHWFYSELKRHNVSHYIYYLATENI
                     HLVLENDNTVLIKGQGKVVNVRFSKNKCLIEATLKGFKSGELSFYEYRKNLATAGVFR
                     WITNIHENKRYYYTFDNSLLFTENIQNTTQIFPH"
BASE COUNT          413 a          271 c          226 g          305 t
ORIGIN      
        1 aggccaaaaa gcccgttttc agcacctgtc gtttcctttc ttttcagggg gtgttttaaa
       61 taaaaacatt aggttacggc gaagaagaac ggaaacgcct taaaccggaa aattgtcata
      121 aatagcgaaa acccgcgcgc cttccgcccc gtaacctgtc ggatcgccgg aaaggacccg
      181 caaaatgata ataattatca tctacatgtc acaacgtgca tctacgccat caaaccacgt
      241 caaataatca attatgacgc aggtatcgta ttaattgatc tgcatcaact taacgtaaaa
      301 acaacttcag acaatacaaa tcagcgacac tgaatacagg gcaacctcat gtcaacgaag
      361 aacagaaccc gcagaacaac aacccgcaac atccgctttc ctaaccaaat gattgaacaa
      421 attaacatcg ctcttgttca aaaagggtcc gggaatttct cagcctgggt cattgaagcc
      481 tgccgccgga gactgtgctc agaaaaaaga gtttcgcctg aagcaaacaa agaaaagagt
      541 gacattactg aattgctcag aaaacaggtt agaccagatt gaagcaattt agataatcgt
      601 gcagactacg ccccctcata tcacatggaa ggtactacaa tggctcaggt tgccattttt
      661 aaacaaatat tcgataaagt gcgaaataat ttaaactatc actggtttta ttctgaacta
      721 aaacgtcaca atgtctcaca ttacatttac tatttagcca cagagaatat tcatcttgtt
      781 cttgaaaacg ataatacggt tttaataaaa ggacagggta aggttgtaaa tgtaagattt
      841 tcaaaaaata aatgccttat agaagccacc ttaaaaggat tcaaatcagg agagttatca
      901 ttttacgaat acaggaaaaa tcttgctaca gcaggggttt tcagatggat tacaaatatc
      961 cacgaaaaca aaaggtatta ctataccttt gataattcat tactctttac tgagaacata
     1021 cagaacacta cacaaatatt tccgcactaa atcataacgt ccggtttctt ccgtgccaga
     1081 accggactcg ctggcatgat gaaatatgtg tacccggtaa ccccggtgtg catcgttttt
     1141 gattattccc gcacactcgc gcagaaggag ttccccgtcg ggctacggtc tctgttaata
     1201 cgggaatacg gcgac
//
LOCUS       DAZPDU010000392         1215 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZPDU010000392 DAZPDU010000000
VERSION     DAZPDU010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.396"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..>1215)
                     /locus_tag="ACHURS_004703"
     CDS             complement(<1..>1215)
                     /locus_tag="ACHURS_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFR1240228.1"
                     /translation="KAPFDIRLQIRDEGLILNDSGGRSIHFEPLFPGEISYSRSESLW
                     LARGGVAAQHSSQPLSALWQVLPEDVRLSPHVYLATNSLQGPWWILSWPERVPGADEV
                     LPPEPPAYRVLTGVVDGFGRTLAFHRAAEGDVAGAVTGVMDGAGRRFHLVLTTQAQRA
                     EEARKPHTASLSSPDSPCPLSAPSFPDTLPAGTEYGADNGIRLEAVWLTHDPAYPDEQ
                     PTAPLARYTYTAGGELRAVYDRSGMQVRGFTYDAEHAGRMVAHHYAGRPESCYRYDDT
                     GRVTEQVNPEGLDYRFEYGESRVIITDSLNRREVLYTEGEGGLKRVVKKEHADGSITR
                     SEYDEAGRLKAQTDAAGRRTEYRLHMASGKLTSVILPDGRTVRYGYNSQRQVTSVTYP
                     DGLRSSREYDEKG"
BASE COUNT          191 a          459 c          337 g          228 t
ORIGIN      
        1 cccttctcat catactcccg gctgctgcga agcccgtccg ggtacgtcac cgacgtcacc
       61 tgccgctggc tgttatagcc ataccgtacc gtcctgccgt ccggcagtat cactgaagtc
      121 agctttccag acgccatatg cagtcggtac tccgtccgcc gtcccgccgc atccgtctgc
      181 gccttcagcc tccccgcctc atcatactcg ctgcgggtga tgctcccgtc ggcatgctcc
      241 ttcttcacca cccgcttcag gccaccctcg ccttccgtgt acagcacctc ccgccggttc
      301 aggctgtccg tgatgattac ccggctctct ccgtactcaa agcggtagtc cagcccctcc
      361 gggttgacct gctccgtcac ccgaccggta tcatcatacc ggtagcagct ctccggcctc
      421 cccgcataat ggtgcgccac catccgcccg gcgtgctccg catcataagt aaacccgcgc
      481 acctgcatcc cgctgcggtc atacaccgcc cgcagttcac caccggccgt gtacgtgtag
      541 cgcgccagcg gcgcagtcgg ctgttcatcc gggtatgccg ggtcgtgtgt cagccatacc
      601 gcctccagcc ggataccgtt atcggcaccg tactctgtac cggcgggcag cgtgtcgggg
      661 aaggaaggag ctgagagagg gcagggactg tcaggggaag ataacgaagc ggtgtgcggt
      721 ttacgggcct cttccgcccg ctgcgcctgt gtggtcagca ccaggtgaaa acggcggccc
      781 gcgccgtcca tcacccccgt caccgcgccc gccacgtcac cctccgctgc ccggtgaaag
      841 gccagcgtcc gcccgaagcc gtccaccacg cccgtcagca cccggtatgc gggcggctcc
      901 ggcggcagca cctcgtccgc ccccggcacc cgctccggcc agctgagtat ccaccacggc
      961 ccctgaaggc tgtttgtcgc caggtacaca tgcggactca ggcgaacatc ttccggcagc
     1021 acctgccaga gcgcgcttag cggctgcgaa ctgtgctgcg ccgctactcc gccccgcgcc
     1081 agccagagtg actcgctgcg gctgtagctt atctcgccgg gaaacagcgg ctcaaagtga
     1141 atgctcctgc cgccgctgtc gttgagtatc aggccttcat cgcgtatctg taagcggata
     1201 tcgaagggcg ctttc
//
LOCUS       DAZPDU010000393         1213 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZPDU010000393 DAZPDU010000000
VERSION     DAZPDU010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.397"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            142..495
                     /locus_tag="ACHURS_004704"
     CDS             142..495
                     /locus_tag="ACHURS_004704"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240229.1"
                     /translation="MFSIGNVSINCTINPEDNNLLPHYVWIYGHTSTQLNQYDSDIIF
                     IINDKKYPAPSVDGTRMNKNAWVYFIDAISEATQFDVLVNGKKVDSYTANIKNVKKTL
                     GNKFYDSCWNTWFQE"
     gene            621..1160
                     /locus_tag="ACHURS_004705"
     CDS             621..1160
                     /locus_tag="ACHURS_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HFR1240230.1"
                     /translation="MNWHYEKNGVRYDNVTEEDIIERIRRGELNASTLVWQQGMLEWQ
                     PVSATSLAEILKQSEVPPALPGHRIPGGVVWTLAFAPLIGYILEMWTAGLNGMSFERA
                     YDAVSEGQYWFISLILNIALGYLDERKLRKSGVDTATFGWLAWLIPVYLWRRAKILGQ
                     KPAYFWVWLVMLILVLFTV"
BASE COUNT          362 a          222 c          270 g          359 t
ORIGIN      
        1 ttaagattaa tttatttaat tttggggaga aaactcatga aactgttaaa cattctttta
       61 gctgttgttt taactgtaag tgcaaccgta gcacaaacag aatcggcatt gtagacgcgt
      121 aacagtattg ctggcactga tatgttcagt atcggtaatg tttctattaa ctgcacgatt
      181 aatccagaag acaacaattt gctaccacac tatgtgtgga tttacggtca tacctccacc
      241 cagctaaatc agtacgattc tgatattatt tttatcatca acgataaaaa gtatccggcc
      301 ccatctgtcg acggaacacg aatgaataag aatgcatggg tatactttat cgatgctatc
      361 agtgaagcca cgcaattcga cgttttagta aacggtaaga aagtcgacag ctataccgca
      421 aatatcaaaa atgtgaaaaa gactttgggt aacaaattct acgacagctg ctggaacacc
      481 tggttccagg aataattgat aacgtggtgt aaaacacctc agtttaattt aatacaatga
      541 gtgaaaatga atattataaa aatatttttt gtttcagatg tcatatattt tattaactga
      601 atgattaaca ggagaatcag atgaactggc attatgaaaa aaatggtgta cgttatgata
      661 acgtaacaga ggaagatatt atcgaacgta tccggcgtgg tgaactgaat gcatcaacac
      721 tggtgtggca acagggcatg ctggaatggc agccagtttc agccacatca ctggcggaaa
      781 tactgaaaca aagtgaagtt cccccagccc ttccgggtca ccgcattccc ggaggcgttg
      841 tctggacgct ggcatttgcc cctttgattg ggtacatact tgagatgtgg actgcagggc
      901 tgaacggtat gtcatttgaa cgggcttatg atgcagtcag cgaaggccag tactggttta
      961 tctctctgat tctgaatatt gctctgggat atctggatga acgaaaactc cgtaaatctg
     1021 gtgttgatac tgcaacattt ggctggctgg catggttgat cccggtttat ctgtggcgtc
     1081 gggcaaaaat cctcggacaa aaacctgcgt atttctgggt ctggttggtt atgctgatac
     1141 tggtgttatt tacggtctga tattctttat cgcagagttt actaaataat caggttatta
     1201 gtgttcttaa cag
//
LOCUS       DAZPDU010000394         1210 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZPDU010000394 DAZPDU010000000
VERSION     DAZPDU010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.398"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..478)
                     /gene="umuC"
                     /locus_tag="ACHURS_004706"
     CDS             complement(<1..478)
                     /gene="umuC"
                     /locus_tag="ACHURS_004706"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFR1240231.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGMGIAQTKTLAKLANHAAKKWQRQ"
     gene            complement(478..897)
                     /locus_tag="ACHURS_004707"
     CDS             complement(478..897)
                     /locus_tag="ACHURS_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFR1240232.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
BASE COUNT          351 a          305 c          249 g          305 t
ORIGIN      
        1 tctgccgctg ccattttttt gccgcatgat tggcaagctt agccagcgtt ttggtctggg
       61 cgatccccat accaacagta agatgggtac gttgtagcac cgttgcgcga atttctctgc
      121 caaaatcagt cagatcgcga caattacgca cacctgtcag atcgcagaat gcctcatcaa
      181 tactgtaaat ctcgacgcgg ggcgatagtt cttccagcgt cgacatcacc cgattgctca
      241 tgtctgcgta aagctcataa ttgctgctaa agcaaaccac accacagcga cgaaacagat
      301 ctttttgttt gaaccaggga tcgcccattt taacacccag cgcctttgcc tcagcgtttc
      361 gggcgataac gcaaccgtca ttattcgata gcacaaccac cggtttaccc cataaatcag
      421 ggcgaaacac cgtctcacag ctggcataaa acgcgtttac atcacagaga gcaaacatca
      481 gcgcatcgcc ttaacgacgt ggatcaccac accaaagaca tccagcgtat cttcgctact
      541 gatggtaatg ggcgagtacg cactgttcat gggaataagc tgtaccgtcg ggcgtagttg
      601 caattttttc accgtaaact cgccgtcaac agcagcgatg acaatatcac catggctggc
      661 ggtaatagcg ctatcgacaa tcagtaaatc accgtcacta attccaccat caatcataga
      721 atcaccactt gctttgacga agtaagtcgc gctgggatgc tggatcaaca gttgattcag
      781 atcgatgcgc tgttcgacgt aatctgctgc cggtgaagga aagccacact gaacaagatc
      841 gctaaatagc ggaaaagtca caatttcgcg gagatccgca ggcttgataa acaacataat
      901 aatctgcctg aagttatact gtttttatat acagtaatct gttcttgcca gcagatcaat
      961 actgattcag gctatcaata tttgtcgctg catagactgc tgatttttcg ttctcttatc
     1021 ctgtgctcac gtggccttct ggcgacgacg ctcatccagc agaaatgaaa aatatcaccc
     1081 ggcaaaaaaa tagaatagaa gcattcgcca taatgacatt aaacattgtt tgatatttat
     1141 catattaata gaaataaaga cattgacgca tcccgcccgg ctaactatga attagatgaa
     1201 gtaaaattta
//
LOCUS       DAZPDU010000395         1200 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZPDU010000395 DAZPDU010000000
VERSION     DAZPDU010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.399"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            12..1094
                     /locus_tag="ACHURS_004708"
     CDS             12..1094
                     /locus_tag="ACHURS_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HFR1240233.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYARLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNNQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGMVYQF"
BASE COUNT          337 a          259 c          270 g          334 t
ORIGIN      
        1 aagggctcac gatgaaaaaa ttaacagtgg caatttctgc tgtagctgca tcagtactga
       61 tggcgatgtc tgctcaggca gctgaaattt ataataaaga cagtaacaag ctggatctgt
      121 acgggaaagt taatgctaag cactacttct cctctaatga tgcagatgat ggtgatacta
      181 cttatgcccg tcttggcttc aaaggtgaaa cccaaatcaa cgatcaactg actggtttcg
      241 gtcagtggga atatgaattc aaaggcaacc gcgctgaatc tcaaggttcc tccaaagata
      301 aaacccgtct tgccttcgct ggcctgaaat tcggtgacta cggctccatc gattacggcc
      361 gtaactacgg tgtagcatac gacatcggtg cgtggactga cgtcctgcca gaattcggtg
      421 gtgacacttg gactcaaaca gatgtgttca tgactggtcg caccactggt gttgcaactt
      481 atcgtaacaa cgacttcttt ggtctggttg atggtctgaa ctttgctgct cagtatcagg
      541 gtaaaaatga ccgcactgac gtaactgaag ctaatggtga tggtttcggt ttctccacta
      601 cttatgagta tgaaggattc ggtgtaggtg caacctatgc taaatctgac cgcactaata
      661 atcaggttat ctacggtaac aacagcctga atgcatctgg tcaaaatgct gaagtatggg
      721 cagctggtct gaaatatgat gcgaacaaca tctatctggc taccacctat tctgaaaccc
      781 agaacatgac tgtttttggt aataaccata ttgccaacaa agcacaaaac ttcgaagtag
      841 ttgcacaata tcagttcgac ttcggtctgc gtccgtccgt tgcttacctg caatctaaag
      901 gaaaagactt gggtgcgtgg ggtgatcagg acctggttga atatattgat gtaggtgcaa
      961 cctattactt caacaaaaat atgtccactt ttgttgatta caaaatcaac ctgattgata
     1021 agagcgattt cacgaaagca tctggcgttg ctaccgatga tatcgttgct gtaggtatgg
     1081 tttaccagtt ctaatttgat tactaaaaga tatgttgcgg gaggctttgc ctccccaaca
     1141 tataagtggc tccctcaagc cacttccttt aggagcacaa ccttgcttct aactatataa
//
LOCUS       DAZPDU010000396         1187 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZPDU010000396 DAZPDU010000000
VERSION     DAZPDU010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.400"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..755)
                     /locus_tag="ACHURS_004709"
     CDS             complement(<1..755)
                     /locus_tag="ACHURS_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFR1240234.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTIGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAVEKIGFDLLIFSEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINMPRIPSMKAILGAGKKPVNQWQASDIGWSQSAPLAEL
                     TGIRVPPQTERKHIILDNDSPEAIAELVEHLKKA"
     gene            1071..>1187
                     /locus_tag="ACHURS_004710"
     CDS             1071..>1187
                     /locus_tag="ACHURS_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240235.1"
                     /translation="MYQRCFDNASETLFVVGKTPRLSRFAFSDDPKWESGHHV"
BASE COUNT          290 a          286 c          289 g          322 t
ORIGIN      
        1 gctttcttca gatgttccac cagctcggca atggcctccg gcgaatcgtt atcgagaatg
       61 atgtgcttac gttctgtttg cggcggtacg cgaatgccgg tcagttcagc aagtggcgcg
      121 ctctggctcc agccaatatc acttgcctgc cactgattta ccggtttttt acccgcgccg
      181 agaatggctt tcatcgaagg aatacgtggc atgttaatat cggaggtgac gcagagcacg
      241 gctggaacag agagctcaat aacttcaaca tcatcttcaa gcgtgcgttc aatcaccagt
      301 gtattgccct gacgctgaat agcactcact gcattaatca ccggaagttg cagaatttct
      361 ccgaccagca agccaacctg ctgggcataa aggtcgccgg aaccttcact aaagatcagt
      421 aaatcgaagc cgatcttttc aactgctgcc gccagcgcct ttgcggtatc gagaggcagt
      481 gcatgttcaa gttgcgcatc ctgcaccaaa tacaggctgt gcggcccgcg ggatagcacg
      541 tctttgcgca ctttcgagtt ctgcaacaat gagccaccaa tggtcagcgc ggctatctca
      601 tcgtcatctg ttgcaagctg gcttgcagct tcaatggcat tgagatcgaa ctggctgatt
      661 ttggcgtcgg cattgtcgaa attcagggtg tattctggag tgacaacaat gtcctgttct
      721 tcaggcacca gcttaaagca ggttattatt ttcatgattt ctcctgtgag tcgcatacgg
      781 aaccatctcc gatggaaggt aaaaatggcc tgaaaatagt gtgcgatagc ggtgcggggt
      841 tttcgacgga gcaagctgtt ttccttgcca acaatcttgc aaaaaaaatg cgatcgctaa
      901 aacaaaagtg caatttgcgg caatatagtt attccatgac cgtacaatct tgcattcagt
      961 gtaaataatc ttgtttagct ctcgctaaac gcggttgtat aaccgcaata cacgccgtat
     1021 tttatccggc atatattgtt tttgcttgct catggaatag ggaggttgtc atgtatcaac
     1081 gctgttttga taacgccagt gaaacgctgt ttgtcgtcgg taaaacgcca cggctttcac
     1141 gttttgcatt tagcgatgat ccaaaatggg agtccggaca tcacgtt
//
LOCUS       DAZPDU010000397         1184 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZPDU010000397 DAZPDU010000000
VERSION     DAZPDU010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.401"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            60..743
                     /gene="tssD"
                     /locus_tag="ACHURS_004711"
                     /pseudo
     CDS             60..743
                     /gene="tssD"
                     /locus_tag="ACHURS_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065915.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
     gene            723..>1184
                     /locus_tag="ACHURS_004712"
     CDS             723..>1184
                     /locus_tag="ACHURS_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240236.1"
                     /translation="MAGASIVAGAVLGRMRLPDLESLEHFGARGAVSGRPFNPELAGG
                     PIENLTTDGVTINREGIAIVEKHIARFGHDPVNEVMINRLKDIEKGKIPPEQVDLNFY
                     THECREYQRYCNLGWETGQPDGDAGYALWNHTGFAPIFPDICYHLTHYKPAA"
BASE COUNT          369 a          236 c          257 g          322 t
ORIGIN      
        1 aaaaaaaaca acaatactat ttctctttca caaaagaatt aacaaaaggt ttttcaagga
       61 tgagtgatat tatttacctg accataaccg gagaacaaca aggatgtatt tcctctcgtt
      121 gtggaacgag cgcatcaata ggtaatcgct ggcagattgg ccatgatgat gaaatcttcg
      181 cgttctcgct ctctaatagt attacgaata caggcaaagg gtcataattg catggcctta
      241 gtttctgtaa attgattgac aaaagctccc cgttacttat taacgctatt aacaataacg
      301 agcagttgtt catggagttt gacttctatc ggatcaatag gtttggtcga tgggagaaat
      361 attacaacat tcaactccgc ggcgcgctct tatcagcaat caatcacctg tttactgaga
      421 acaatctgga tacagaagcc atcactgtca gttattaata tatactctcc aggcatctga
      481 ttgctaatac tgaatttagt tattgggcat ttcccgataa ctacaaccgt ctgtttatac
      541 cacggccaaa aactaaagct ggtaatggtt tcaaaacact taatagtaaa gcggttggca
      601 gactgcttgc tgctgtcggc atatacaatg ggaatatcga aggattcagg gaaacagcag
      661 acaagttggg gggagatgca cctgctggtt atgatcaatt tatggataac aaagggttac
      721 tcatcgctgg tgcttcaata gttgctggag cagttctggg gagaatgaga ctccctgatt
      781 tggaatcact tgagcatttc ggagccagag gagcagtatc aggaagacca tttaatcccg
      841 agcttgccgg tggtccaata gaaaacctga cgacagatgg cgtaacaatc aacagggaag
      901 gtatcgctat tgttgaaaaa catatagcca gatttggtca tgatcctgta aatgaagtga
      961 tgatcaatag actgaaagac attgagaaag gaaaaatccc cccagaacaa gtagatctta
     1021 acttctatac tcatgagtgt cgggaatatc agcgttactg taatcttggt tgggaaacag
     1081 gacaacctga tggtgacgca ggctatgcac tctggaatca tactggattt gcccctatat
     1141 ttccagacat ctgttatcac ttaacccatt acaagcccgc tgcc
//
LOCUS       DAZPDU010000398         1172 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZPDU010000398 DAZPDU010000000
VERSION     DAZPDU010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.402"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..>1172
                     /gene="fimD"
                     /locus_tag="ACHURS_004713"
     CDS             <1..>1172
                     /gene="fimD"
                     /locus_tag="ACHURS_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFR1240237.1"
                     /translation="SHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNKWQHIN
                     TWLERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPVIHGIA
                     RGTAQVTIKQNGYDIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIFTVPYS
                     SVPLLQREGHTRYSITAGEYRSGNAQQEKTRFFQSTLLHGLPAGWTIYGGTQLADRYR
                     AFNFGIGKNMGALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNIQLVGY
                     RYSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLTVTQQL
                     GRTSTLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGRDQM"
BASE COUNT          332 a          262 c          283 g          295 t
ORIGIN      
        1 acagccatta tgcatattta aacctacaga gtgggctaaa tattggtgcg tggcgtttac
       61 gcgacaatac cacctggagt tataacagta gcgacagttc atcaggtagc aaaaataaat
      121 ggcagcatat caatacctgg cttgagcgag acataattcc gttacgttcc cggctgacgc
      181 tgggtgatgg ttatacccag ggcgatattt tcgatggtat taactttcgc ggcgcacaat
      241 tggcctcaga tgacaatatg ttacccgata gccaaagagg atttgccccg gtgatccacg
      301 gtattgctcg tggtactgca caggtcacta ttaaacaaaa tgggtatgac atttataata
      361 gtacggtgcc gccggggcct tttaccatca acgatatcta tgccgcaggt aatagtggtg
      421 acttgcaggt aacgattaaa gaggctgacg gcagcacgca gatctttacc gtaccctatt
      481 cgtcagtccc gcttttgcaa cgtgaagggc atactcgtta ttccattacg gcaggagaat
      541 accgtagtgg aaatgcgcag caggaaaaaa cccgcttttt ccagagtaca ttactccacg
      601 gccttccggc tggctggaca atatatggtg gaacgcaact ggcggatcgt tatcgtgcgt
      661 ttaatttcgg tatcgggaaa aacatggggg cactgggcgc tctgtctgtg gatatgacgc
      721 aggctaattc cacacttccc gatgacagtc agcatgacgg acaatcggtg cgttttctct
      781 ataacaaatc gctcaatgag tcaggcacga atattcagtt agtgggttac cgttattcga
      841 ccagtggata ttttaatttc gctgatacaa catacagtcg aatgaatggc tacaacatcg
      901 aaacacagga tggtgttatt caggttaagc cgaaattcac cgactattac aatctcgctt
      961 ataacaaacg cgggaaatta caactcaccg ttactcagca actcgggcgc acatcaacac
     1021 tgtatttgag tggtagccat caaacttatt ggggaacgag taatgtcgat gagcaattcc
     1081 aggctggttt aaatactgca ttcgaagata tcaactggac gctcagctat agcctgacga
     1141 aaaacgcctg gcaaaaagga cgggatcaga tg
//
LOCUS       DAZPDU010000399         1165 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZPDU010000399 DAZPDU010000000
VERSION     DAZPDU010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.403"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            1..1165
                     /gene="rhsC"
                     /locus_tag="ACHURS_004714"
                     /pseudo
     CDS             1..1165
                     /gene="rhsC"
                     /locus_tag="ACHURS_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="rhs element protein RhsC"
BASE COUNT          289 a          339 c          354 g          183 t
ORIGIN      
        1 aggtgcgcag ctttacttac gatgataaat accggggccg gatggtggcg caccgtcaca
       61 cgggccggcc ggaaatccgt taccgttacg acagcgacgg gcgggtgaca gaacagctta
      121 acccggcagg cttaagctac acgtatcagt atgagaagga ccgcatcacc atcaccgaca
      181 gcctgaaccg ccgtgaagtg ctgcacacgt agggtgaagc cgggctgaag cgggtggtga
      241 aaaaggaaca cgcggacggc agcgtcacgc agagtcagtt tgacgccgtg ggcaggctca
      301 ggacacagac ggatgccgca ggccggacaa cagaatacag cccggatgtg gtgacgggcc
      361 tcatcacgcg catcaccacg ccggatggca gggcatcggc gttttactat aaccaccaca
      421 gccagttaac gtcagccacc gggcctgacg ggctggaaat gcgccgcaaa tatgatgaat
      481 ccggccgtct gattcaggaa accgcccctg acggcgatat cacccgctac cgttatgata
      541 atccacacag tgacttaccc tgcgcaacgg aagatgccac cggcagccgg aaaaccatga
      601 catggagccg ttacggtcag ttgctgaccg tcactgactg ttccggttat gtaacccgtt
      661 atgaccatga ccgttttggt caggtgacgg cggtgcaccg cgaggaaggg ctgagccagt
      721 accgcgcata cgacagccgt ggacagttaa ttgcggtgaa agacacgcag ggccatgaaa
      781 tgcggtatga atacaacgcc gccggtgacc tgaccgccgt cattgccccg gacggcagca
      841 gaaacgggac acagtacgat gcgtggggaa aagccatctg taccacgcag ggcgggctga
      901 cgcgcagtat ggaatacgat gctgccggac gggtcatccg tctgaccagt gaaaacggca
      961 gccacaccac cttccgttac gatgtactcg accggctgat acaggaaacc ggctttgacg
     1021 gccgcacaca gcgttatcac cacgacctga ccggcaaact tatccgcagc gaggatgagg
     1081 ggctggtcac ccactggcac tatgacgaag cagaccgcct cacgcaccgc accgtgaagg
     1141 gtgaaaccgc agagcggtgg cggta
//
LOCUS       DAZPDU010000400         1161 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZPDU010000400 DAZPDU010000000
VERSION     DAZPDU010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.404"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            248..493
                     /gene="yahM"
                     /locus_tag="ACHURS_004715"
     CDS             248..493
                     /gene="yahM"
                     /locus_tag="ACHURS_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HFR1240238.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPIVGIRQIRLIT"
     gene            complement(510..>1161)
                     /locus_tag="ACHURS_004716"
     CDS             complement(510..>1161)
                     /locus_tag="ACHURS_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFR1240239.1"
                     /translation="FMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQTSLASGRR
                     AGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAWCSMRRQS
                     TPQMSTLQQPISASWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTWARLMAWA
                     GIVLASVIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYEGVTQR"
BASE COUNT          351 a          277 c          275 g          258 t
ORIGIN      
        1 gtttcgcagc catgcggtgt ataaaaaatg atctcatgca gatgttttgt gaatgtgtgg
       61 gttgacattc atatgaaaaa aatcataatt ccatcatgtt tgtgtggggt cttttctgta
      121 ccttacgcat cgcactcaag cctgacagaa aatatgctgt aaggctcata tcaaaaaccg
      181 ccattagctc atcaggaaga gcagacgaca accaggattg ttgtatggta cggggttcga
      241 ggcctcgatg gcggtccaac ttttcaaaac tcttctgaat cagatcccct tattatcatc
      301 cctgcaaagt ggaacattgc cattgtttgg ctattcagga cgggtcagac caatgaaaaa
      361 agcccacaca ggggagagtg ggctgaaatg ggaagctaaa gactcaagta aacttatcgg
      421 aaataaggac cacgcattac gggggctatc atcgccgata gtcgggattc ggcaaatcag
      481 gttgattacg tagagagtaa attatctgct caccgctgcg tcaccccttc gtaaatcagg
      541 cgtagcgcga atacaccaat aattgcacca ataacccgac tggcaacgcg ttgcatacgc
      601 ccataagcac gacgcacagc gggcaaagaa aacgcctgac taagaaagac tcgccagata
      661 actgatgcga gcacaatccc cgcccaggcc attaaacgtg cccatgttgg tgtttcggca
      721 tttaatgtta ctgagaaaat actgataaaa aataaaacgg tttgcgggtt agagagatcg
      781 gtaattaatc cgcggcgaaa aaagacatac caggaggcgc taatcggttg ttgtagtgtg
      841 ctcatttgcg gtgttgactg gcggcgcatg ctgcaccacg caaaccataa gagataagcg
      901 ccgccgacga ttctgataag cgaaaaaatc tcctcacact gcgttattag cgttgcaaga
      961 ccaaacaaac ccaacccgga ataaaatgca tcgcccagcg ccacgcccag tccggtcagc
     1021 acccctgcgc gtcgaccgga agccaggctg gtttgtacta ccacaaagag attggctccc
     1081 ggattaaaaa aagtaatcac gaacagtcct acggtcaggt aaacggcgtg caaaggatcc
     1141 atagtgattt catccataaa t
//
LOCUS       DAZPDU010000401         1157 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZPDU010000401 DAZPDU010000000
VERSION     DAZPDU010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.405"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            6..407
                     /locus_tag="ACHURS_004717"
                     /pseudo
     CDS             6..407
                     /locus_tag="ACHURS_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            572..>1157
                     /locus_tag="ACHURS_004718"
     CDS             572..>1157
                     /locus_tag="ACHURS_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFR1240240.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLAGIPA
                     ICLCYSQISQAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPR
                     LNKLVTEVIPSAVTSEGTKANAYKYTERYTAFYRV"
BASE COUNT          322 a          260 c          276 g          299 t
ORIGIN      
        1 ggtgacgagt ctcatcgtct atggaaacga cattcgtgct ggatgcactg gagcaggcgt
       61 tgtgggcccg tcgcccgtct ggcaccatcc atcacagcga taaaggctct cagtatgtgt
      121 cactggccta taaggcgcga ctaaaagaag caaaactgct ggcatcaaca gggagtactg
      181 gtgactcgta tgacaacgcg atggcagaga tcatcaaggg cctttacaaa gcggaggtaa
      241 tacaccgtaa gagctggaaa aaccggacag aagtggagct ggccacactc acgtgggtgg
      301 actggtataa caatcgacga ttgctggaaa ggctgggcca tatcccaccg gcagaagcag
      361 aaaaagctta ttatgcttcc atcggaaatg atgatctggc agcctgagtt cacagataaa
      421 acactctcca ggaaacccgg ggcggttcag ttcacaaaaa cacattaatg cagtaactat
      481 attttccttt ctggtgataa cgtcgggtta tcattagctt cttcagctat tttactttta
      541 tatcccttgt aaacatcatg gagaatccat agtgaataaa gtttattctc ttaaatattg
      601 ccccgtcacc ggggggctta ttgctgtctc tgaacttgcc cgcagggtaa taaaaaagac
      661 atgccgaaga ttaacgcata ttcttctggc tggcattcca gcaatctgtc tgtgttactc
      721 tcagatatcc caggcgggta ttgtccgttc cgatattgcc tatcagattt atcgtgattt
      781 cgccgaaaac aaagggcttt ttgtacctgg tgccaatgat attccggtat atgataagga
      841 cggaaaactt gtgggaagac tgggtaaagc cccaatggcc gatttcagca gtgtgagctc
      901 aaatggcgtt gctacgcttg tatcgcctca gtatatcgtc agcgtaaagc ataacggagg
      961 atatcggagt gtgagctttg gtaatgggaa aaatacatat tcccttgttg accgtaataa
     1021 ccacccttct attgacttcc atgctccacg tctgaataaa ctggttacag aagttattcc
     1081 ctcagcggta acatcagaag gaaccaaagc caatgcttat aaatacactg aacgttacac
     1141 cgctttttat cgggtgg
//
LOCUS       DAZPDU010000402         1145 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZPDU010000402 DAZPDU010000000
VERSION     DAZPDU010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.406"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..187
                     /locus_tag="ACHURS_004719"
     CDS             <1..187
                     /locus_tag="ACHURS_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFR1240241.1"
                     /translation="REYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNN
                     YRIALRKYQHYQRQTCT"
     gene            320..1120
                     /gene="tcyJ"
                     /locus_tag="ACHURS_004720"
     CDS             320..1120
                     /gene="tcyJ"
                     /locus_tag="ACHURS_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR1240242.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
BASE COUNT          318 a          248 c          306 g          273 t
ORIGIN      
        1 gcgtgaatat gtcgttcgtc tgcgccgttt gggtaatcat ctacacgagc aaaatatttc
       61 cctcgatcag ctgcaggacg gtttccttga tgaaatcctc gccccgtggc tgcccaccac
      121 cagcaccaac aattaccgca ttgcgttacg gaagtatcaa cactatcagc gtcaaacgtg
      181 tacctgactt gtgcagaaat cctccagtct gcctgcttct gatatatatt aaataagaat
      241 aagatgtagc ggagttgttt ttgtgtttac aaacaatggc tctacactgc aaacagacat
      301 aacaacattc ggggtgaata tgaaattagc acatctggga cgtcaggcat tgatgggtgt
      361 gatggccgtg gcgctggttg cgggtatgag cgttaaaagt tttgcagatg aaggtctgct
      421 taataaagtc aaagagcgcg gcacgctgct ggtagggctg gaaggaactt atccgccgtt
      481 cagttttcag ggggatgacg gcaaattaac cggttttgaa gtggaatttg cccaacagct
      541 ggcaaaacat cttggcgttg aggcgtcact aaaaccgacc aaatgggacg gtatgctggc
      601 gtcgctggac tctaaacgca ttgatgtggt gattaaccag gtcaccattt ctgacgagcg
      661 caagaaaaaa tacgattttt ctacccctta caccatttcc ggtattcagg cgctggtgaa
      721 aaaaggtaac gaaggcacca ttaaaacagc cgatgatctg aaaggcaaaa aagtgggtgt
      781 cggtctgggc accaactatg aagagtggct gcggcagaat gttcagggtg tcgatgtgcg
      841 tacctatgat gatgacccga ccaaatatca ggatctgcgc gtaggccgta tcgatgcgat
      901 cctcgttgac cgtctggcgg cgctggatct ggtgaagaaa accaacgata cgctggcagt
      961 aaccggtgaa gcattttccc gtcaggagtc tggcgtggcg ctgcgtaaag gaaatgaaga
     1021 tctgctgaaa gcagtgaatg atgcaattgc ggaaatgcaa aaagatggca ctctgcaagc
     1081 cctttccgaa aaatggtttg gtgctgatgt gaccaaataa tcagcataat gacaaaaaag
     1141 ggcgc
//
LOCUS       DAZPDU010000403         1140 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZPDU010000403 DAZPDU010000000
VERSION     DAZPDU010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.407"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..>1140
                     /locus_tag="ACHURS_004721"
     CDS             <1..>1140
                     /locus_tag="ACHURS_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFR1240243.1"
                     /translation="EREIDHLQAQLDKLRRMNFGSRSEKVSRRIAQMEADLNRLQKES
                     DTLTGRVYDPAVQRPLRQTRTRKPFPESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTA
                     EQLELMRSAFRVIRTVREKHACTQCDAIVQAPAPSRPIERGIAGPGLLARVLTSKYAE
                     HTPLYRQSEIYGRQGVELSRSLLSGWVDACCRLLSPMEEALHGYVLTDGKLHVDDTPV
                     PVLLPGNKKTKTGRLWAYVRDDRNAGSTLAPAVWFAYSPDRKGIHPQTHLAGFSGVLQ
                     ADAYAGFNELYRDGRITEAACWAHARRKIHDVHVRTPSALTEEALKRIGELYAIEAEI
                     RGMTAEQRLAERQLKTKPLLKSLESWLREKMKTLSRHSELAKAFA"
BASE COUNT          250 a          323 c          361 g          206 t
ORIGIN      
        1 ggagcgcgaa atagaccatc tgcaggcgca gctggataaa ctccgccgga tgaacttcgg
       61 cagtcgttcc gaaaaagtct cccgccgtat cgcacaaatg gaagccgatc tgaaccggct
      121 tcagaaagag agcgatacgc tgactggtag ggtgtatgac ccggcagtac agcgtccgtt
      181 gcgtcagacc cgcacccgta agccgttccc tgaatcacta ccccgtgacg aaaagcgact
      241 gttgcctgcg gcgccgtgct gcccgaactg cggcggttca ctgagctatc tgggcgagga
      301 taccgccgaa cagctggagt tgatgcgtag cgccttccgg gttatccgga cggtacggga
      361 aaaacatgcc tgtactcagt gcgatgccat cgtgcaggca cctgcacctt cgcggcccat
      421 cgagcggggt atcgccggac cggggctgct ggcccgcgtg ctgacctcga agtatgcaga
      481 gcacaccccg ctgtatcgcc agtcagaaat atacggccgg caaggtgtgg agctgagccg
      541 ttcactgctg tcgggctggg tggatgcatg ctgccggctg ctgtctccga tggaagaggc
      601 gcttcatggc tatgtgctga ctgacggtaa gctccatgtt gatgacacgc ctgtcccggt
      661 gctgttgcca ggcaataaga aaacgaagac cgggcggttg tgggcgtatg ttcgtgacga
      721 ccgtaacgcc gggtcaacgc tggcgccggc ggtgtggttc gcttacagcc cggacagaaa
      781 aggcatccat ccgcagaccc atcttgcggg gttcagtggt gtactgcagg cggatgcata
      841 cgccgggttc aacgagctgt accgggatgg ccggataacg gaagccgcct gttgggctca
      901 cgcccgccgt aaaatccacg atgtgcacgt tcgcaccccg tcagccctga cggaggaagc
      961 gctgaaacgg atcggcgaac tgtacgccat cgaggcagag ataaggggaa tgacggcgga
     1021 acagcgcctt gccgaacgtc agttgaaaac gaaaccgctg ctgaaatccc tggaaagctg
     1081 gctgcgtgaa aagatgaaaa ccctgtcgcg acactcagaa ctggcgaaag cgttcgcata
//
LOCUS       DAZPDU010000404         1116 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZPDU010000404 DAZPDU010000000
VERSION     DAZPDU010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.408"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            83..1114
                     /locus_tag="ACHURS_004722"
     CDS             83..1114
                     /locus_tag="ACHURS_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HFR1240244.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEHPVFYEDEVDIHLNPKIGADWQLRGQQKRVVTPGQNEKYYLAGA
                     LHSGTGKVSCVGGNSKSSALFISLLKRLKATYRRAKTITLIVDNYIIHKSRETQSWLK
                     ENPKFRVIYQPVYSPWVNHVERLWQALHDTITRNHQCSSMWQLLKKVRHFMETVSPFP
                     GGKHGLAKV"
BASE COUNT          303 a          285 c          301 g          227 t
ORIGIN      
        1 agatcacttt gagggaactt agcccggatt gtgcgatctg atcaatcgcc aaatcaaaac
       61 aaatcaccaa ccggactgag caatgccgat catagcacca atttcccgtg acgaacgacg
      121 cctgatgcag aaagccatcc ataaaacaca cgataaaaat tatgcccgca gactgactgc
      181 catgctgatg ctgcaccggg gcgaccgtgt cagcgacgtt gccagaacgc tctgctgcgc
      241 ccgttcctct gttggacgct ggattaactg gttcacgcag tcgggtgttg agggactgaa
      301 atcattacct gccgggcgtg cccgtcgctg gccgtttgag catatctgca cactgttacg
      361 tgagctggta aaacattctc ccggcgactt tggctaccag cgttcacgct ggagtacaga
      421 actgctggca ataaaaatca atgagataac cggttgccag ttaaatgccg gaaccgttcg
      481 ccgctggttg ccgtctgcgg ggattgtgtg gcgaagggct gcgccaactc tgcgtatccg
      541 tgacccgcat aaagatgaaa agatggcagc aatccataaa gcactggacg aatgcagcgc
      601 agagcatccg gtcttttatg aagatgaagt ggatatccat cttaatccca aaatcggtgc
      661 ggactggcaa ctgcgcggac agcaaaaacg ggtggtcacg ccgggacaga atgaaaaata
      721 ttatctggcc ggagcgctgc acagcgggac aggtaaagtc agctgtgtgg gcggcaacag
      781 caaaagttcg gcgctgttca tcagcctgct gaagcggctt aaagcgacat accgtcgggc
      841 gaaaaccatc acgctgatcg tggacaacta cattatccac aaaagccggg aaacacagag
      901 ctggctgaag gagaacccga agttcagggt catttatcag ccggtttact cgccatgggt
      961 gaatcatgtt gaacggctat ggcaggcact tcacgacaca ataacgcgta atcatcagtg
     1021 cagctcaatg tggcaactgt tgaaaaaagt tcgccatttt atggaaaccg tcagcccatt
     1081 ccccggaggc aaacatgggc tggcaaaagt gtagcg
//
LOCUS       DAZPDU010000405         1115 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZPDU010000405 DAZPDU010000000
VERSION     DAZPDU010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.409"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..643)
                     /locus_tag="ACHURS_004723"
     CDS             complement(<1..643)
                     /locus_tag="ACHURS_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR1240245.1"
                     /translation="MSKISDLNYSQHITLADNFKQKSEVLNTWRVGMNNFARNAEGQD
                     NTRNILDPKTFLEFLVKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEIMKF
                     VMNNPEGERADLSKVEIEITLSAFTTMGTRQGHTAIIFQQPDGSTNRYEGKFFERKDE
                     SSLHLITNKILACYQREANKEIARLLNIPQELNNSQDLNNSQVSCKDSVDSTIT"
     gene            complement(766..>1115)
                     /gene="tabA"
                     /locus_tag="ACHURS_004724"
     CDS             complement(766..>1115)
                     /gene="tabA"
                     /locus_tag="ACHURS_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFR1240246.1"
                     /translation="LSISWSHYLFYLISEDMTEPHEARRTEYHARYLDIQIVLKGQEG
                     MTFSTQPAGTPDTDWLADKDIAFLPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAP
                     AQVRKAVVKMLMA"
BASE COUNT          323 a          252 c          196 g          344 t
ORIGIN      
        1 ccgtaatcgt agaatcaact gaatctttac aactcacctg cgagttattc aagtcctgag
       61 aattatttaa ctcctgcgga atatttaata gacgagctat ttccttgtta gcttcgcgtt
      121 ggtaacatgc cagaatcttg ttagtaatca ggtgtaatga actctcatct tttctttcaa
      181 agaactttcc ttcataacga ttagtcgaac catcaggttg ttgaaatata atagccgtat
      241 gtccttgacg agtccccata gtagtgaaag cggaaagtgt gatttctatt tccaccttag
      301 ataaatccgc tcgttcccct tcaggattat tcataacaaa cttcattatc tcactgccat
      361 cattattttt gatataagat gaggactcaa tatgagccat catatttctt cccgcttcgt
      421 tggagcgttt gctaaaatcc acataaccta gagtaaatat ttttaccaaa aattccaaaa
      481 atgtcttagg atcaaggata tttcttgtgt tatcctgccc ctcggcatta cgggcaaaat
      541 tattcattcc aacacgccag gtatttaaaa cttcactttt ttgtttaaaa ttgtcggcta
      601 atgtaatgtg ttgagaataa ttcaaatctg atattttgct catgacgaac tccttgagtt
      661 aataagaacg tctaagcatt acaaatttta ctcacaaaaa aatatcaaaa aacgctcttt
      721 tcacaaactc gccgtaaacc tggagttatt tacggcgaaa agtcatcacg ccatcagcat
      781 cttcactact gctttgcgaa cctgggctgg tgcacccact gcgcacagcg gtttatgcac
      841 ttcccccgga taaaacacaa caaaatcacc ttcattaagg ataactgttt tctcatcaac
      901 gccttccggc aaaaatgcga tgtctttatc agctaaccag tcggtatccg gcgtgcctgc
      961 aggttgcgtg ctgaaggtca tgccttcctg accttttaac acaatctgaa tgtcgagata
     1021 gcgggcatgg tactccgtac ggcgagcttc gtgcggctcg gtcatatctt ccgagataag
     1081 ataaaacagg taatgactcc aacttattga tagtg
//
LOCUS       DAZPDU010000406         1088 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZPDU010000406 DAZPDU010000000
VERSION     DAZPDU010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.410"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            212..>1088
                     /gene="nadA"
                     /locus_tag="ACHURS_004725"
     CDS             212..>1088
                     /gene="nadA"
                     /locus_tag="ACHURS_004725"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFR1240247.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMLTLQAECSLDLGCPVEEFNAFCDAHPDHTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSFGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCR"
BASE COUNT          294 a          259 c          265 g          270 t
ORIGIN      
        1 caggcatcct caatttcact ttgtaaacct gatgacatcg tcagagctta ctgtgcaagc
       61 aactctacgt cggtggaatt aggcgtaatt cgagtgactt ttccccacca ttcgactatc
      121 ttgtttagca tataaaacaa attacgccga tagtagcgaa tattacgcta atgtcggttt
      181 taacgttaag cctgtaaaac gagatggtaa gatgagcgtg atgtttgatc cagacacggc
      241 gatttatcct ttccccccga agccgacgcc gttaagcatt gatgaaaaag cgtattaccg
      301 cgagaagata aaacgtctgc taaaagaacg taatgcggtg atggttgccc actactatac
      361 cgaccccgaa attcaacaac tggcagaaga aaccggtggc tgtatttctg attctctgga
      421 aatggcgcgc ttcggtgcaa agcatcccgc ttctacgttg ttagtcgctg gggtgagatt
      481 tatgggagaa accgccaaaa ttctcagtcc ggaaaaaaca attctgatgc tgacacttca
      541 ggctgaatgt tcactggatc tcggctgccc tgttgaagaa tttaacgcat tttgcgatgc
      601 ccatcccgat catactgtcg tcgtctacgc caacacttct gctgcggtaa aagcgcgcgc
      661 agattgggtg gtaacttcaa gcattgccgt cgaacttatt gatcatctgg atagttttgg
      721 tgaaaaaatc atctgggcac ccgacaaaca tctggggcgt tacgtgcaaa aacagacggg
      781 cgcagacatt ttatgctggc agggtgcctg tattgtgcat gatgaattta agactcaggc
      841 gttaacccgc ttgcaagaag aatacccgga tgctgccata ctggtgcatc cagaatcacc
      901 acaagctatt gtcgatatgg cggatgcggt cggttccacc agtcaactga tcgctgctgc
      961 gaaaacattg ccacatcagc ggcttatcgt ggcaaccgat cggggcattt tctacaaaat
     1021 gcagcaggcg gtgccagata aagagttact ggaagcacca accgcaggtg agggtgcaac
     1081 ctgccgca
//
LOCUS       DAZPDU010000407         1088 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZPDU010000407 DAZPDU010000000
VERSION     DAZPDU010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.411"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            529..>1088
                     /gene="fimB"
                     /locus_tag="ACHURS_004726"
     CDS             529..>1088
                     /gene="fimB"
                     /locus_tag="ACHURS_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HFR1240248.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTTSNAGRFYG"
BASE COUNT          333 a          189 c          233 g          333 t
ORIGIN      
        1 tccaggataa aaaggaatgt aacaatctca tggcgtaagc tgacgaacca ggcaggaata
       61 atcgctaggg acctaagcat tagcatgata atagccacta agaaattact gtgttccatg
      121 aaatagccat tttgtggcaa atggagttga ctaataatgt catatgtgag acggctagtt
      181 gaacgaatat taaattttgc tgaatttttt atgttgattt tacttgttac agaacatatc
      241 acatgatata tagataagat tagttgcatt aatgatgagg gttattatta gattcgtatc
      301 aggttgataa atatataaag gtacatagca tgcaagagca tggcgtttgt atggcaaagt
      361 tattataagt aactatcgct actccattta agttcgctca gaagaactgg tccacttacg
      421 ttagttatta agcaaacgtt cgcttttata aacataatca ggattaaaat gttggattat
      481 tgctaaccca gcacagctag tgcgcgtctg taattataag ggaaaacgat gaagaataag
      541 gctgataaca aaaaaaggaa cttccttacc catagtgaaa tcgagtcact ccttaaagca
      601 gcaaataccg ggcctcatgc agcacgtaat tattgtctga ctttgctttg ttttattcat
      661 ggtttccggg cgagtgaaat ttgtcgattg aggatttcgg atattgatct taaggcaaag
      721 tgtatatata tccatcgatt aaaaaaaggc ttttcaacaa cgcacccgct attgaacaaa
      781 gaagttcagg ctttaaaaaa ctggttgagt atccgtactt cttatccgca tgctgagagc
      841 gagtgggtat ttttatcacg taaggggaat ccgctttctc ggcaacagtt ttaccatatt
      901 atctcgactt ccggtggtaa tgccgggttg tcactggaga ttcatccgca catgttacgc
      961 cattcgtgtg gttttgcttt ggcgaatatg ggaatagata cgcgacttat ccaggattat
     1021 cttgggcatc gcaatattcg tcatactgtc tggtatacca ccagcaatgc agggcgtttt
     1081 tacggcat
//
LOCUS       DAZPDU010000408         1087 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZPDU010000408 DAZPDU010000000
VERSION     DAZPDU010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.412"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            35..190
                     /locus_tag="ACHURS_004727"
                     /pseudo
     CDS             35..190
                     /locus_tag="ACHURS_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
     gene            294..>1087
                     /locus_tag="ACHURS_004728"
     CDS             294..>1087
                     /locus_tag="ACHURS_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1240249.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSL"
BASE COUNT          315 a          223 c          254 g          295 t
ORIGIN      
        1 ataacagatg tctggaaata taggggcaaa tccaggtact gccgaaaata ttcagataga
       61 gctgagggat gaccaggatg ctgcgttaaa aaatggcgat agcaaaacgg ttattgttga
      121 tgagatcact cgtaatgcac agtttccact taaggcaaga gctatcacgg tgaatggaaa
      181 cgcaagctag ggaacgatcg aggcgctaat caatgtgatc tacacctggc aataacgtcg
      241 attcgatttt cttagccttc tttttaaatc aatttttaac cttaggatat atcatgggta
      301 aaacaatcag tataaaggtc ctgttcggta tttatcttct attaatggca ggcaaagttt
      361 ttgccttcag ttgtaatgtc gatggcgggt caagtattgg agcaggtaca acctctgttt
      421 atgttaatct cgaccctgta atacagccgg gccagaatct ggttgtagac ttgtctcagc
      481 atataagttg ctggaatgat tacggcggct ggtacgacac tgatcatata aacctggtac
      541 aaggctcagc tttcgcaggc tccctacagt catataaagg atcgctgtac tggaataatg
      601 tgacctaccc cttcccgttg acgacaaata ccaacgtatt ggatattggt gataagacac
      661 cgatgccatt accgttaaaa ctctatatta ccccagttgg ggcggccggt ggagttgtca
      721 taaaagcggg ggaagtcatt gcccgtattc atatgtataa aattgcaaca ttaggaagcg
      781 gaaatccgcg taattttacg tggaatatta tttcaaataa cagtgttgtt atgccaacag
      841 gcggctgcac cgttgatagt cgtaatgtca ctgtggatct cccggacttt cctggaagcg
      901 cagaaatacc ccttggcgta tattgctcaa gcgaacaaaa gctctcattt tatctttcgg
      961 gtgccaccac tgacagtagc cgacaagtgt ttgctaacac agcacctgat gcaacaaaag
     1021 catcgggagt gggtgtcact ctaatgcgta atggaaaaat actggctacg ggagaaaatg
     1081 tctccct
//
LOCUS       DAZPDU010000409         1077 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZPDU010000409 DAZPDU010000000
VERSION     DAZPDU010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.413"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..906)
                     /locus_tag="ACHURS_004729"
     CDS             complement(<1..906)
                     /locus_tag="ACHURS_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter YfaL"
                     /protein_id="HFR1240250.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSIADGQWLVFSDMTNNASGGAVFLQQGAEFTLSPENETGMTLFAN
                     NTVTGEYYNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLSVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPPGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDI"
BASE COUNT          307 a          243 c          214 g          313 t
ORIGIN      
        1 aatatcaaag gtaacttcac ttaagccgag atacataaag ccacccgccg cagtggaagg
       61 accatctcca tagcctgctg cgctgttgtt ctcatccact aacacgccgc cgttctggct
      121 gtagctgtca tcaacagaaa tatcaataag atagggagcc gtcgcgctat tggtatatat
      181 cgccccgcca taaccttcgg cagtgttatt tgtaaaggca gtgttattta ttatcgtata
      241 acctggagga tgcttgctgt cgctattatt atcggtaaca tcgattgccc cgccatcgcc
      301 atcactgtaa cttgttgatg tatatgcctg gttgttatca aaaataacat cacttaaata
      361 gacgtcatta ttaatggtat aaattgcgcc acctttgcca tcattagcga tattattgcg
      421 aaacatggcg ttagtgacac ttaaatcgac ggcaccagta tcgttagtac cagaagaata
      481 gattgcgcca ccatagccgc ctgcgacgtt accggaaaaa atgacatcag taagattcag
      541 cgttgagttt tctttagcaa atatggcccc gccgttataa tattctcctg taacggtgtt
      601 attggcaaac agagtcattc cagtttcgtt ttctggtgat aatgtaaatt ccgctccttg
      661 ttgcaaaaat acggccccac cgctggcatt attggtcata tccgaaaaaa ccagccattg
      721 cccatcggcg atactccagt cctgcgtaat gcctgaaagg ctttgcctgc tggcgtgaca
      781 actcgctttg atatcatatc cctggcataa atcaatggcc gccgcgacac cgttagcaga
      841 gaaaagagat gcaatcattg acgggagtag agataaatac tccttgcgta gaaagataat
      901 ccgcatatta atctgaccat cattttatat aagacggcat atttaaacgc attatacaat
      961 atgggttatc tttcttttgt aaattgtcca acccgatgca atggctattt tgttattatt
     1021 tgatgaataa tatcagtgcg tcataattca agttaataac cttaagggat accagtt
//
LOCUS       DAZPDU010000410         1060 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZPDU010000410 DAZPDU010000000
VERSION     DAZPDU010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.414"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(229..900)
                     /gene="queE"
                     /locus_tag="ACHURS_004730"
     CDS             complement(229..900)
                     /gene="queE"
                     /locus_tag="ACHURS_004730"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFR1240251.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVISRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          310 a          244 c          223 g          283 t
ORIGIN      
        1 tgattaaaaa ggtaaatatt taaaataact ctatacaaat taaaatctac caaactttac
       61 cgcaataatt ttcactccag cgaaaaatta atgccacaga atttgtagag aatgctaatg
      121 attagccctg gatgggtaaa atggaggagt tttcagaggc gacaaacaat ataaatgagt
      181 agaagtcttt acaggttcca cccatttata acgcttataa atgtttaatc aggcaatatt
      241 tagatattta tgtgtttgca tcgacaaacg ccaattacgc gcaatgcagg tttcaatgca
      301 caaacgtgtg gcatcatcct tttggctaat cggctgcagt gcaatgactc gcggtttatc
      361 atcggtcagc gttgccaata gttcatccag tgcttcaata tcgcgtacgc gccccactgg
      421 atgcttgatt tcgttggctc gctccagtgc ctgtgacaac acgtcatagc cgccgcgcat
      481 gttcagcttt ggcgatacgg taacccaggt attcggtgtg cagcgtacct catgagtacc
      541 actggtttcg atctggcagc taaaaccgtt cttttcgagc agatcagtca gtggtagcaa
      601 atcatgaatg caaggctcac cacccgtaat caccacatgc cgcgcggtgt atccctggcg
      661 actaatgaca gccaacaagt cttcactgct cgccgccccc cacttatcac tctctttggt
      721 cttcgccaga atgctgaaaa gggagacttc ccgatcctca agcttttccc aggtgtgttt
      781 ggtgtcgcac caggcacagc caaccgggca tccctgtaaa cgaataaaaa tggcggggac
      841 gccggtaaag taaccctcac cttgcagggt ctggaacatc tcgttaatcg ggtactgcat
      901 agcattctct gtgaagtgga taattgttaa ttattgcaga tcctgccaca acaatcatgt
      961 cttattaaca ttctgttaca ggcagattta aagaaaaatg cgaaacatat cgttaataat
     1021 taaagggagt aacgtattat gtcagaagaa aataaagaaa
//
LOCUS       DAZPDU010000411         1049 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZPDU010000411 DAZPDU010000000
VERSION     DAZPDU010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.415"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..>1049)
                     /locus_tag="ACHURS_004731"
     CDS             complement(<1..>1049)
                     /locus_tag="ACHURS_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFR1240252.1"
                     /translation="ASVSFRIAAPAALSRIELTPGYFQITATPHLAVYDPTVQFEFWF
                     SEKRIADIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKSAFVEAVGQ
                     PSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVSNEITQTVNK
                     KLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIENTPDGMQSQ
                     VLLAADRIAMVNPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNPSAFSLTPD
                     GKLTAKNADISGSVNANAGTLNNVTVNENCTIKGMLEATQVRGDFVKAVSKSFPKQAG
                     TWGNTETPNGTVTVT"
BASE COUNT          231 a          299 c          246 g          273 t
ORIGIN      
        1 atggtgactg taaccgtccc gtttggtgtt tccgtgttac cccacgtacc agcctgtttc
       61 ggaaatgatt tggatacagc tttaacgaag tcacctctga cctgagtcgc ctccagcatg
      121 cccttaatcg tacagttttc atttaccgtg acattgttga gcgtcccggc gttcgcattc
      181 acactgccac tgatatccgc atttttagcg gtcagctttc cgtccggagt cagggaaaat
      241 gccgatggat ttccaccgct ggtaatggtg ggggccgtca ggcgtttcag gaacacgtcg
      301 ttcatgaata tctgatcgcc ctgaccaaca aacatcggtt ttgtgttgcc attcgcagga
      361 ttaaccatcg caatcctgtc cgccgccagc agcacctgac tctgcatgcc gtcaggggtg
      421 ttctcaatac cggcaccaat acccgcgata taaaggcgtc cgtcctgcat ctgctgcagc
      481 ttcacagccc acatgctgtt caggttatta tttgtatcaa cctgaacctt ctgtatctgc
      541 tgaattgccg cactctggtc ttccagtttc ttattgacgg tctgcgtgat ttcattgctg
      601 acatccgtaa tggacgtcct gatttcagcc aggtcaggcg caagctgacc gttatcaatc
      661 tgcgtccaca gctcctgagc cagatgggtt ttccctatct cgcctttgaa aaaatccaga
      721 taaccggatg catcatcact cggctgacca acagcctcca cgaatgccga tttgccaacg
      781 gtgttcacac tgcggatata aaagtaataa tcatggcccg gtttgatatt gatactggcg
      841 gctatccagt acagcgccgt gccaagatag cgtgctgtgg tttcaacctg cctgatatcc
      901 gcaatccgct tttccgagaa ccagaactca aactgtaccg tcggatcata aaccgcaaga
      961 tgcggcgtgg cggttatctg aaaatagccc ggcgtcagct caatccgcga cagcgctgcc
     1021 ggtgcggcaa tccggaacga taccgacgc
//
LOCUS       DAZPDU010000412         1049 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZPDU010000412 DAZPDU010000000
VERSION     DAZPDU010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.416"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..120
                     /locus_tag="ACHURS_004732"
     CDS             <1..120
                     /locus_tag="ACHURS_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFR1240253.1"
                     /translation="EFQQVYKSVLNVLWNWILFRKFSSPEQVENVAAQLLEFA"
     gene            120..410
                     /locus_tag="ACHURS_004733"
     CDS             120..410
                     /locus_tag="ACHURS_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFR1240254.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHDPETSVLAHYRLAGTCGTAIK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            407..961
                     /locus_tag="ACHURS_004734"
     CDS             407..961
                     /locus_tag="ACHURS_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFR1240255.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLRDMINGKQKSWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPKWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIEKKVIAF"
BASE COUNT          299 a          201 c          298 g          251 t
ORIGIN      
        1 gagtttcagc aggtttataa atctgttctg aatgtgctgt ggaactggat tctgttccgt
       61 aaattttcct ctccggaaca agtcgaaaat gtggccgcgc agctgctgga gtttgcgtaa
      121 tggtggattt acgtaaagcg gcgcgggggc agatgtgcac cgtcagaatt cctggctact
      181 gcaatcacga tccggaaacg tctgtgctgg cgcattaccg actggcggga acgtgcggaa
      241 cagcgataaa gccacacgat atgcaggcag cgattgcctg tagctcgtgc cacgatttaa
      301 tcgacgggcg ggtaaaaacc agcgattaca ccaaagaaga attacgcctg atgcatgcag
      361 aaggtgtttt tcgcacacaa gaaatctgga gaaaggaggg atatttatga tttacccaac
      421 gaatacagga aaaagcggag aacaccttcg tctcaccacg ctggaaagtg tctggattca
      481 gggaaaacta cgtatgtggg ggcgctggtc gtatattggt ggcggtaaga cggggaatat
      541 gtttaacctg atgttgacct ctaaaaagct gacaaaaacg gcaattaacg aggcgctccg
      601 gaggatgaaa aaagcaggtc tgaacaagtc tgaacttgag gcttttttgc gggatatgat
      661 taacggtaag caaaagagct ggctggcgca ttgtactgat gcagaggcgt tatgtattga
      721 tcgggtcata agtgaggtgc tggcagagca tccaggattg attagcgtcc ttcggcaacg
      781 gtatgagggg cgggggatga ccaaacgcaa aatggctgaa ctgctgaatg atgcacaccc
      841 gaaatggagt ttaagaacct gtgaaagacg cattgagcat tggctaaagg tggcagaatt
      901 tattttgtac aaaccaatgg ttatggcttt tggtatagag aaaaaagtta ttgctttttg
      961 acgtaaaaac tgcttcaatt cttgtacgct tcgcaaagct gtaccgcgag gcgaatagca
     1021 gacatggaca tttgaaagag cccgcttta
//
LOCUS       DAZPDU010000413         1046 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZPDU010000413 DAZPDU010000000
VERSION     DAZPDU010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.417"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            108..257
                     /locus_tag="ACHURS_004735"
                     /pseudo
     CDS             108..257
                     /locus_tag="ACHURS_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612150.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            284..388
                     /locus_tag="ACHURS_004736"
                     /pseudo
     CDS             284..388
                     /locus_tag="ACHURS_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            728..985
                     /locus_tag="ACHURS_004737"
     CDS             728..985
                     /locus_tag="ACHURS_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071468 - cellular response to acidic pH
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development regulator YmgB/AriR family
                     protein"
                     /protein_id="HFR1240256.1"
                     /translation="MHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAVNALFCERE
                     IINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
BASE COUNT          357 a          180 c          198 g          311 t
ORIGIN      
        1 atcatacaga taattactgg ttaaccctat atagggcatt ttaaagtgtt gtgatcttac
       61 ccagcaatag tggacacgcg gctaagtgag taaactctca gtcagaggtg actcacaaga
      121 caaaacagtt tcaaccagta aaaaaacgta aacaccattc gcatgaattt cgtagttaag
      181 atctgaagct tgcagaatgc atcggtgtta ctgccggagt ccgtgaactt agtctgcatg
      241 aatcaccaca gctttacatc tgtcgcagta aacagcaaaa tcagcaaact tacttaatta
      301 tatcgagtgc gattacaatc gctggcgatg gtacagttgg tgtggcggtc tcagtccgga
      361 acaatttgaa aatcagaatc tcacttaaag cggcatccat tttacaaaag atggccgtct
      421 gtgtatttgt aatgaatacc ttctacatgt ttagagtgca taatccacga caaggcgata
      481 tcctaaaaat tagctagcag gcaactttga tggcatagat aatgttttta gtaaaaaaga
      541 gttgactgaa tataatacag cctcttccta cataagaagg aatgtgctca caaaaaaaaa
      601 ccattagtct gcttcctgtt cttacaataa tcagtattat gtattttatt tatatagcaa
      661 aatatgcttg ttacacctgt cataattagg catggtaaaa ttattgactg aaccctgggg
      721 gaggaagatg cacagtgatt attataatat ggtttttggt gaaaaattgg ccaatatttt
      781 atatgaggct aactctcaat ttttttacga gagaaatgtc atagaagagg cagtcaacgc
      841 tttattctgt gagcgtgaaa taataaacaa caagaacata ataaagaagc tgatgttctt
      901 tttgtcagat gttaatcaca ccaagaaaga tgttgttcag agcgcactta atattatcat
      961 tgacattaca tcaggtgata tttgagattt tacataaaag ataatctctc aaggagtctt
     1021 atctgaaaac gaaatatata gtccct
//
LOCUS       DAZPDU010000414         1029 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZPDU010000414 DAZPDU010000000
VERSION     DAZPDU010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.418"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            289..>1029
                     /locus_tag="ACHURS_004738"
     CDS             289..>1029
                     /locus_tag="ACHURS_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240257.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNAKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYISDQYDLVQHQLQQINACHTNTTYTNADYSKVVAQLVSLITNIETIS
                     STQLTQQTQSILNQINNIRYEKNKSAECRIIVVANPKPDKAIITKISVEEGIPITFSV
                     QTMFSDTNFIAEQRADLPTNIKDIQSLYQKMTKLYIEHSENKNRMKVFAGANFIDFNM
                     TGQNLSGFVLTLSRFYFEDLLNINFTDAN"
BASE COUNT          398 a          195 c          150 g          286 t
ORIGIN      
        1 cacatctaaa acatcacaaa aaaaatcact tcacacacta tcagagaaca taataacttt
       61 ttaaaatgag cgcattttga tagttgttaa cgatttcctt tacgatacta tcactaccct
      121 ttttttatac acaaatatta ccgggtggag ataaaaggga aatcaaaggt aattatatca
      181 ggaagatata acgcattaca tttattgtgt gtagaagaaa atccattcct gttctaatta
      241 tccaatttaa acatcttagc ataaaacaaa tgatgaataa ggaattttat gtcaattgac
      301 tttaccccag gtataataaa tacatatcac ggcgatattt ataactgcac aacaaatacc
      361 gataatgcta aaacgccaga cacaccgaag tggccttgcg ataattggga agaacaacaa
      421 cccattaatt ccacattctc tggagaagga tatatctctg atcaatatga tttagtgcaa
      481 caccaacttc aacaaattaa tgcgtgccac acgaacacta catatacaaa tgcagactac
      541 tcaaaagttg tggctcaact tgtaagtctt attactaaca ttgaaacgat aagttcgaca
      601 caacttacgc aacagacaca atcaatactt aatcagatta ataacattag atatgaaaaa
      661 aacaaaagtg ctgaatgtcg aatcattgtt gtcgctaatc ctaaacccga caaggcaatc
      721 ataactaaaa ttagcgtaga agagggaata cccataacat tcagtgtcca aaccatgttt
      781 tcagatacta attttattgc tgaacaacga gctgacttac caactaatat aaaagatatt
      841 caaagcctgt atcaaaaaat gacgaagtta tatattgagc acagtgaaaa taaaaacagg
      901 atgaaagtct ttgccggagc caactttatt gatttcaata tgacaggaca gaacctgtcc
      961 gggttcgttc taaccttgtc aaggttttat ttcgaagatc tacttaatat aaatttcact
     1021 gacgcaaat
//
LOCUS       DAZPDU010000415         1023 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZPDU010000415 DAZPDU010000000
VERSION     DAZPDU010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.419"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(77..865)
                     /locus_tag="ACHURS_004739"
     CDS             complement(77..865)
                     /locus_tag="ACHURS_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR1240258.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFAA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(905..>1023)
                     /locus_tag="ACHURS_004740"
     CDS             complement(905..>1023)
                     /locus_tag="ACHURS_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFR1240259.1"
                     /translation="KEAIRFASAVAAMKCTQPGGRAGIPNREQTESFLSLYA"
BASE COUNT          255 a          263 c          239 g          266 t
ORIGIN      
        1 atcgctggca ggtttttttt gcttatggaa aacaaaatcc gctgaaattc ccagcggatt
       61 ttccgaaatt tcaatttcag gatacttctt gctcggcaac caccagttca acattctctt
      121 cacttaacaa ctggcagaaa gaattgggag gcggggcatc agtaaaaaaa gccctgacat
      181 tccgtgcatt gccaattgaa acggcagcag acgcggcaaa cttcgtatga tcggccacta
      241 acaatgtatt ccgcgcatgt ttaatcatcg ttctcgcgac taacgcttca tttacatcaa
      301 attccagtag ggtgccgtca tgttcaatgg cacctatact ggtgattaaa taatctgcgc
      361 ggaagccttc aataaaatcc acggctcctg ggccgataat cccgccgtta tgagcgcgta
      421 aagtgcctcc cggcaccatc acttcaatat cctgattctt ataaagaatc tgtgccacac
      481 gtaggctgtt agtgataatg cgtaaatcac gccggttgag taatgccctg gcaacggctt
      541 ctacggttgt accgatggtg ataaagacgg tacagcgttc gggaaggtaa tcggcgactg
      601 cctcagcgat cgcccgtttc tcggcggtga gcgaaagttc ccgttgctca aaagcggtat
      661 tcatgacgct ggagacgcgc ccggcccctc catgatgacg ggtgatcaag ccctgctcac
      721 tgagttttcg gatatcccgg cgcacggttt gcgttgaaac atccagcagt tgtgccagtt
      781 cttcaatatt catataaccg cgttctgcga ttagatgaac gagttggtca tggcgtggat
      841 tacctgtcac ttcgatgata ctcatgaata ctcccttgcc cctcctcggt agatgtttac
      901 cattttacgc ataaagtgac aaaaatgatt cggtttgctc acgattagga atgccggccc
      961 gaccgccagg ctgggtacat ttcatcgcgg caaccgcact ggcaaaacga atagcctctt
     1021 ttg
//
LOCUS       DAZPDU010000416          994 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZPDU010000416 DAZPDU010000000
VERSION     DAZPDU010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.420"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            86..808
                     /locus_tag="ACHURS_004741"
     CDS             86..808
                     /locus_tag="ACHURS_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001382845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR1240260.1"
                     /translation="MLSGLLLLCLPFSSFACATTSHISFSYAARQRMQNRARLLKQYQ
                     THLKKQASYIVEGNAQSRRALRQHNREQIKQHPEWFPAPLKASDRRWQALAENNHFLS
                     SDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKILEAKLPRTANE
                     MYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRTGETIRVSRL
                     AEPFWQDHFLGARRILTEETIL"
BASE COUNT          234 a          256 c          261 g          243 t
ORIGIN      
        1 gttcaggtac gggccaggat acactgcaac atcaatgcat gaccagccat tattgagaat
       61 gtctttgccg tcgattccct cttttgtgtt gtcgggatta ctgttgcttt gcttgccgtt
      121 ttcttcattt gcctgcgcca ccacatcaca tatctctttc agctacgccg cccgccagcg
      181 gatgcaaaac cgtgcgcgtt tattaaaaca gtaccaaacc catctgaaaa aacaggccag
      241 ctacattgtg gaaggcaatg cccaaagcag aagggcgctg cgccagcaca accgggagca
      301 gataaaacag catccagaat ggtttcctgc gccgctcaag gcgagtgaca gacgctggca
      361 ggcgctggcg gaaaacaacc actttttaag cagcgatcac ctgcataaca tcaccgaagt
      421 ggcgattcac cgtctggagc agcagcttgg caagccttac gtctggggcg gcacgcggcc
      481 cgatcaaggc ttcgattgta gtgggttagt tttttatgcc tacaacaaga tcctcgaggc
      541 gaagctcccg cgtacggcca atgagatgta tcactaccgt cgggcaacca ttgtggcgaa
      601 taacgacctg cgccggggag atttgctgtt tttccatatc cacagccgcg agattgccga
      661 ccatatgggg gtgtatctgg gcgacggtca gtttattgag tcaccgcgca ccggggaaac
      721 cattcgggta agtcggttag ctgaaccttt ctggcaggat cattttttgg gcgcgcggcg
      781 gatattgacg gaagagacga ttttgtagga cggataaggc gtttacgccg catccggcgg
      841 tggtgcgcag atgcctgatg cgacgctgac gcgtcttatc atgcctacaa atctgtgcgt
      901 ttttgcgagc cgctttcctg ttattccatc acccagggaa agatcatcag aagtacactt
      961 tttgtactaa ataattcgca ttttatgttt aaaa
//
LOCUS       DAZPDU010000417          988 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZPDU010000417 DAZPDU010000000
VERSION     DAZPDU010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.421"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            44..988
                     /gene="yfdF"
                     /locus_tag="ACHURS_004742"
                     /pseudo
     CDS             44..988
                     /gene="yfdF"
                     /locus_tag="ACHURS_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937827.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdF"
BASE COUNT          381 a          172 c          173 g          262 t
ORIGIN      
        1 gtaatatata tttgtaacaa atcaatcaaa atggaataaa atcatgctac catctatttc
       61 aatcaacaat accagcgcaa cttacccaga atccattaat gaaaataaca atgatgaagt
      121 taatggatta gtacaagagc tcaaaaacct ttttaatggt aaggaaggaa taagcacctg
      181 tattaaacat ctacttgagc ttataaaaaa cgccatacga gtaaacgacg atccttaaga
      241 tttaatatta ataattcctc agttacttat attgatattg gctccaatga tacagaccat
      301 attactattg gtatcgacaa ccaagaacca atagaattac ctgcgaacta taaagacaaa
      361 gaactcgtcc gtactatcat taatgacaac atagttgaga agactcatga tatcaataac
      421 aaggaaatga tcttcagcgc attaaaagaa atatatgatg gagatcctgg ttttatcttc
      481 gataagatat cacacaaact cagacatacg gtaacggaat ttgatgagag cgggaatagc
      541 gaaccaacgg acttatttac ctggtacggt aaagataaaa aaggcaactc tctcgctatt
      601 gtaattaaaa ataaaaacgg aaatgattac ttatctctcg gttactacga tcaggacgac
      661 taccacattc aaagaggaat tcgtattaat ggtgatagtc tcacccaata ttgtagtgaa
      721 aaagccagga atgcttcagc aatgcttcag cgtggtttga aagcagtaaa gctatcatgg
      781 cagaatcatt tgcaacaggt tccgatcatc aggttgtaaa cgagctcaac ggggaaagac
      841 tgagagaacc taacgaagtt tttaaacgtt taggtcgagc aataagatat aattttcaag
      901 tggacgatgc aaaatttaga cgcgacaatg taaaagaaat aatttctaat ttattcgcta
      961 acaaagttga cgttgaccat cctgaaaa
//
LOCUS       DAZPDU010000418          986 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZPDU010000418 DAZPDU010000000
VERSION     DAZPDU010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.422"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            230..>986
                     /gene="gspA"
                     /locus_tag="ACHURS_004743"
     CDS             230..>986
                     /gene="gspA"
                     /locus_tag="ACHURS_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HFR1240261.1"
                     /translation="MSTRREVILSWLREKRQTWRLCYLLGEAGSGKTWLAQQLQKDKH
                     RRVITLSLVVSWQGKAAWIVTDDNAAEQGCRDSAWTRDEMAGQLLHALHRTDSRCPLI
                     IIENAHLNHRRILDDLQRAISLIPDGQFLLIGRPDRKVERDFKKQGIELVSTGRLTEH
                     ELKASILEGQNIDQPDLLLTSRVLKRIALLCRGDRRKLALAGETIRLLQQAEQTSVFT
                     AKQWRMIYRILGDNRPRKMQLAVVMSGTIIAITC"
BASE COUNT          280 a          199 c          238 g          269 t
ORIGIN      
        1 gcatctttat tatgattcgc cagatggaag gggaaacgta gtgtgggcac gatgtatgtt
       61 ctaataaaat agattgggtg ggttgtatat tgacgttagt ataaatagta ttaatgccgc
      121 ttgcatgaat caagcaaaat ttatttatat tagtaatatg tatttaatta ataaatacat
      181 acattatttt tatgattaag gataatcaat aattaaaaca ggaagttcta tgtctacgag
      241 aagagaagtt attctttcct ggttgcgtga gaaacgtcag acctggcgtc tgtgctattt
      301 gttgggtgag gctggaagtg gaaaaacctg gctggcgcag caactgcaaa aagataaaca
      361 tcgccgtgtg attactttaa gcctcgttgt ttcctggcaa ggtaaggccg catggatcgt
      421 taccgacgat aacgcggctg aacagggctg ccgtgacagc gcctggacgc gagatgagat
      481 ggcggggcaa ttactgcatg cgcttcatcg aactgatagt cgctgccctc ttattattat
      541 tgaaaatgct cacctgaatc accgccgcat acttgatgat ctccaacgcg cgatatctct
      601 tattcctgac ggtcaatttt tgctgatcgg cagacccgat cgaaaagtcg aacgtgattt
      661 taaaaaacag ggcattgaac ttgtctcgac aggacgtctg acggagcacg aacttaaggc
      721 aagcatcctt gaaggtcaga atattgacca acccgatctc cttttaacct ccagagttct
      781 gaaacggata gctttattat gtcggggcga tcgcagaaag ctggcgcttg ccggcgaaac
      841 aattcgctta ttacagcaag ccgagcagac cagcgttttc actgcaaaac aatggcgaat
      901 gatttaccgc atattgggtg ataaccgccc ccggaaaatg cagcttgccg tggtaatgag
      961 tggaaccatt atcgctatta cctgtg
//
LOCUS       DAZPDU010000419          974 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZPDU010000419 DAZPDU010000000
VERSION     DAZPDU010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.423"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..>974
                     /gene="tssI"
                     /locus_tag="ACHURS_004744"
     CDS             <1..>974
                     /gene="tssI"
                     /locus_tag="ACHURS_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFR1240262.1"
                     /translation="CISQFRYSAQIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRT
                     QYEVYDYPGRFKGAHGQNFARWQMDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQAN
                     LNREWQVVASELHGEQPQAVPGRSGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQS
                     AVVTGPAGEEIFCDEHGRVRVKFNWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRV
                     GQEVIVDFLNGDPDQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFD
                     DATGKEQVYIHVQKNMNTEVLNNRTTDVINNHAEKIGNNQAITVTNNQ"
BASE COUNT          250 a          261 c          298 g          165 t
ORIGIN      
        1 tctgcatcag ccagttccgc tacagcgcac aaatccgtcc ttcttccgtg gtgaccaaag
       61 actacacctt taaacgaccc ggctgggcag ggcgttttga tcaggaaggc cagtaccagg
      121 attaccagcg cacacagtat gaagtgtatg actaccccgg acgtttcaag ggtgctcacg
      181 ggcagaactt tgcccgctgg cagatggatg gctggcgcaa caacgcagaa gtggcgcgcg
      241 gaacaagccg ttctccggag atatggccgg gacggcgaat tgtgctgacg gggcatccgc
      301 aggcgaacct gaaccgggaa tggcaggtgg tggcaagtga actgcacggc gagcagccgc
      361 aggcggtgcc gggacgcagt ggttcaggaa ccacgctgaa taaccacttt gcggtaatac
      421 cggcagaccg gacatggcgg ccacagccgt tgctgaaacc gctggtggat ggcccgcaga
      481 gcgccgtcgt gacgggaccg gcaggcgagg aaatcttctg tgatgaacat ggccgcgtgc
      541 gggtgaaatt taactgggac cgctataacc cgtcaaacca ggacagttca tgctggatcc
      601 gtgtggcaca ggcgtgggca ggcaccggat tcggtaacct tgcgataccg cgcgtgggtc
      661 aggaggtcat tgtggacttc ctcaacggcg atccggacca gccgattatt atggggcgta
      721 cctaccacca ggaaaaccgc acacccggta gcctgccggg gacgaagacg cagatgacca
      781 ttcgttcgaa aacctataag ggcagcgggt ttaatgaact gaagtttgac gatgcgacag
      841 ggaaagaaca ggtctacatc cacgtgcaga agaacatgaa caccgaggtg ctgaataacc
      901 gcaccacgga tgtgataaac aaccatgccg aaaaaatagg taacaaccag gcgatcaccg
      961 ttaccaataa ccag
//
LOCUS       DAZPDU010000420          968 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZPDU010000420 DAZPDU010000000
VERSION     DAZPDU010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..968
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.424"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            1..968
                     /gene="espL4"
                     /locus_tag="ACHURS_004745"
                     /pseudo
     CDS             1..968
                     /gene="espL4"
                     /locus_tag="ACHURS_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
BASE COUNT          345 a          185 c          170 g          268 t
ORIGIN      
        1 ttctttctct gcaaatatta ataatacagc ccagacaaat gaacaccaaa ccctgagtga
       61 attgttttat aaagaactcg aggataaatt ttctggcaag gagctggcga cgcctctatt
      121 aaaaagcttc tcagagaact gtagacaaaa tggtcgtcat atctttagca acaaggattt
      181 tgtcattaaa ttttccacgt ccgtcttaca agctgataag aaagaaatta cgataattaa
      241 taaaacgaaa acacgacact cactcaaacc attgctccaa tatttgaaga atacctaatg
      301 gaaattttac ctcaacgctc agacactctt gataaacaag aattaaacct aaaatcagat
      361 agaaaagaaa aagaattccc aagaattaaa cttaatggtc aatgttattt tccggggcga
      421 ccccaaaacc gtatagtatg ccgacacatt gctgcacaat atattaatga tatttatcag
      481 aatgttgatt acaaacccca tcaagatgat tattcttcag ctgaaaaatt tctcactcac
      541 ttcaacaaaa aatgcaaaaa ccagactttg gcgttgattt ccagccgtcc tgaggggcgt
      601 tgcgttgctg cctgcggtga tttcgggcta gttatgaaag catattttga caagatggaa
      661 tcaaatggca tcagtgttat ggcagccata ttactggtgg ataaccatgc tttgacggtc
      721 cggctaagaa taaagaacac aactgaagga tgtacccatt acgtggtttc ggtttatgat
      781 cctaatgtaa ctaacgacaa aataagaatc atgagcgaaa gcaaagagga tattaaacac
      841 tattctctga tggattttat gaatgtagat tatagcctcc tgaaatggtc aaatgatcat
      901 gttattaacc aatctgttgc aataattcca gcacttccga aagaacagct attgatatta
      961 aaaggatc
//
LOCUS       DAZPDU010000421          967 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZPDU010000421 DAZPDU010000000
VERSION     DAZPDU010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.425"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..499
                     /locus_tag="ACHURS_004746"
     CDS             <1..499
                     /locus_tag="ACHURS_004746"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240263.1"
                     /translation="EIKKRLYSFVKKETNDWEKWLQIEFAHYLTSKRKYELHREVTIY
                     LDPIDYPDSKLAKVDLILREKSTPSDDYIFIEFKCTKGVVPLVRGLNADKNKISAIRQ
                     CNYKMRNYLGVGFHLFCEPDDIEFMEKYVTEELKGSYAVFKLCECPVRYNCQCEFNEI
                     GVVLF"
     gene            complement(645..>967)
                     /locus_tag="ACHURS_004747"
     CDS             complement(645..>967)
                     /locus_tag="ACHURS_004747"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240264.1"
                     /translation="FALVSLMEKLILLLLLPLTTSAAIPDFKCWDIHNDSFSTDMSFY
                     VTRYLGKMPPPYGAFHYETAPDEKFYRELVITNEAFDDGYNYGSRYLNKEGTLYDDIK
                     CKRL"
BASE COUNT          327 a          156 c          177 g          307 t
ORIGIN      
        1 tgagataaaa aaaagactat attctttcgt gaaaaaagaa actaatgatt gggaaaagtg
       61 gctgcaaata gaatttgctc attatctaac ttcaaaaagg aagtacgagc tacatcgtga
      121 agttacgata tacctcgatc caatagatta tcctgatagt aaacttgcta aagtagattt
      181 gatactgcgt gaaaaatcca caccctctga tgattatatt tttattgagt ttaaatgtac
      241 taaaggtgta gtaccattag ttaggggctt aaatgctgac aaaaataaaa ttagtgctat
      301 aagacagtgt aactacaaaa tgcgtaatta tcttggtgtc ggatttcatc ttttttgtga
      361 accagatgat atcgagttta tggaaaagta cgtaactgaa gaacttaaag gctcttacgc
      421 cgtgtttaaa ttatgcgagt gtccagtaag atataattgc caatgtgaat ttaatgaaat
      481 tggtgtggta cttttttaaa tgcggtgcgg gtatgatcat tttgttcaca cacaattgat
      541 taaacccgct gccgaaaacc ctcgatagta ccgccagtat ggagggaccc attgaatgca
      601 atggttaata tactttgtaa gctatataca atgaatcatg agtatcaaag ccgcttacat
      661 ttaatatcat cgtacaaagt tccttctttg tttagatatc tcgaaccgta attatatcca
      721 tcatcaaatg cttcattggt tattaccagt tccctgtaaa atttctcatc aggtgctgtt
      781 tcataatgaa atgcaccata aggcggcggc attttaccga gatatcttgt aacgtagaaa
      841 ctcatgtcag tactaaatga atcgttatga atatcccagc atttaaagtc tggaatagct
      901 gcacttgttg ttaaaggtaa taataatagc aatatcaatt tttccataag agataccaat
      961 gcaaatg
//
LOCUS       DAZPDU010000422          965 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZPDU010000422 DAZPDU010000000
VERSION     DAZPDU010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..965
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.426"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..>965
                     /locus_tag="ACHURS_004748"
     CDS             <1..>965
                     /locus_tag="ACHURS_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFR1240265.1"
                     /translation="ETHLDFLKQYGDFENGIPVHDTIARVVSCISPAKFHECFINWMR
                     DCHSTDDKDVIAIDGKTLRHSYDKSRRRGAIHVISAFSTMHSLVIGQIKTDEKSNEIT
                     AIPELLNMLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEKKFP
                     LTELNNPAHDSSAMSEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSI
                     IAEQKKEPEMMVRYYISSADLTAEKFATAIRNHWHVENNLHWRLDVVMNEDDCKIRRG
                     NAAELFSGIRHIAINVLTNDKVFKAGLRRKMRKAAMDRNYLASVLA"
BASE COUNT          316 a          176 c          229 g          244 t
ORIGIN      
        1 gaaacacatc tcgatttttt gaagcaatat ggtgattttg aaaatggcat tcctgttcac
       61 gataccattg ccagagttgt atcctgtatc agtcctgcaa aatttcacga gtgctttatt
      121 aactggatgc gtgactgcca ttctacagat gataaagacg tcattgcaat tgatggaaaa
      181 acgctccggc actcttatga caagagtcgc cgcaggggag cgattcatgt cattagtgcg
      241 ttctcaacaa tgcacagtct ggtcatcgga cagatcaaga cggatgagaa atctaatgag
      301 attacagcta tcccagaact tcttaacatg ctggatatta aaggaaaaat catcacaact
      361 gatgcgatgg gttgccagaa agatattgca gagaagatac aaaaacaggg aggtgattat
      421 ttattcgcgg taaaaggaaa ccaggggcgg ctaaataaag cctttgagaa aaaatttccg
      481 ctgacagaat taaataatcc agcgcatgac agttccgcaa tgagtgaaaa gagtcacggc
      541 agagaagaaa tccgtcttca tattgtttgc gatgtccctg atgaacttat tgatttcacg
      601 tttgaatgga aagggctgaa aaaattatgc gtggcagtct cctttcgctc aataatagca
      661 gagcaaaaga aagagcccga aatgatggtc agatattata tcagttctgc tgatttaacc
      721 gctgagaagt ttgcgacagc gattcgaaat cactggcacg tggagaataa tctgcactgg
      781 cgtctggacg tggtaatgaa tgaagacgac tgcaaaataa gaagaggaaa tgcagcagaa
      841 ttattttcag ggatacggca cattgctatt aatgttttga cgaatgataa ggtattcaag
      901 gcagggttaa gacgtaagat gcgaaaagca gccatggaca gaaactatct cgcgtcagtc
      961 cttgc
//
LOCUS       DAZPDU010000423          961 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZPDU010000423 DAZPDU010000000
VERSION     DAZPDU010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..961
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.427"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..>961
                     /gene="htrE"
                     /locus_tag="ACHURS_004749"
     CDS             <1..>961
                     /gene="htrE"
                     /locus_tag="ACHURS_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HFR1240266.1"
                     /translation="WASGQNRSNYSIGYSNSASWGSYSVSAQRSWNEDGDTDDSVYLS
                     FTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNARVSYSVNTGYTM
                     NKASKDLSYVGGYASYESPWGTLAGSVSANSDNSRQVSLSTDGGFVLHSGGLTFSNDS
                     FSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRIALDINDLENDVE
                     LKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADIYDEQGNVIGNVG
                     QGGQAFVRGIEQQGNISIKWLEESKPVSCLAHYQQSSEAEKIAQ"
BASE COUNT          278 a          195 c          238 g          250 t
ORIGIN      
        1 ctgggcttcc ggacaaaatc gtagcaatta ctctattggc tacagtaaca gtgcatcctg
       61 gggcagctac agtgtcagtg cccagcgttc atggaatgaa gacggcgaca ctgacgatag
      121 cgtttatctc agtttcacca ttcctattga aaaattactt ggcactgaac aacggacttc
      181 aggtttccag agtattgata ctcaaatgag cagtgacttt aagggtaata accaactcaa
      241 cgtcagtagc agtggctata gtgataacgc tcgcgtcagt tatagcgtaa atactggcta
      301 tacgatgaat aaagccagca aagatttgag ttatgttggg ggttatgcca gctatgaatc
      361 accgtgggga accctggcag gttcagtttc tgcaaatagc gataacagtc gtcaagtgtc
      421 tctcagcacc gacggtggtt ttgtattgca tagcggcggc ctgaccttca gtaatgacag
      481 ttttagcgac tccgatacac tggcggtagt tcaggctcca ggtgctcaag gagcgcgaat
      541 aaattatggc aacagtacta tcgatcgatg gggttatggt gtcaccagcg ctctttctcc
      601 ttatcatgaa aaccgtatcg cgctggatat caacgatctt gagaacgatg ttgaattaaa
      661 aagtaccagt gcagtagctg taccgcgtca gggttcagtc gtctttgctg attttgaaac
      721 cgtgcaaggg caatcagcca ttatgaacat cacacgaagt gatggtaaaa atattccatt
      781 tgctgcagat atttatgatg agcaaggcaa tgtcattggt aatgttggac agggtggaca
      841 agcatttgtt cgtggcattg agcagcaggg aaatatcagc attaaatggc tcgaagaaag
      901 taaacccgta agttgtcttg cgcattatca acaaagttca gaagcagaaa aaatagcgca
      961 a
//
LOCUS       DAZPDU010000424          959 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZPDU010000424 DAZPDU010000000
VERSION     DAZPDU010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.428"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..304)
                     /locus_tag="ACHURS_004750"
     CDS             complement(<1..304)
                     /locus_tag="ACHURS_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFR1240267.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWR"
BASE COUNT          296 a          221 c          203 g          239 t
ORIGIN      
        1 tgcgccacag cggtgggcat accttcatgc tgtacagcat ctggtttttg tcgttctccc
       61 ccagttcaaa ccaggtcacc acacctttca cccggcgctg tacgtcatcg ccctgccata
      121 ccgtcaggta ggccattttg tccagcacct gcgcaaattc aagggagaga aactgctggc
      181 tgaccagaga gagatcgagg gaaaagagcg aagagagtga ctggttcaaa tgaaaggaaa
      241 ccaccgcaaa agcatccggt ggcaggccgt ccacttccag tgtgaaacgt aatccggttg
      301 acatagcaac ctccgtgaaa atgaataata atccgcacat attgcatgta caacatgtcg
      361 ggcggatgag ataagcgagt cgacatctgg cgttaccggg gaatatcaga aaaatgtcat
      421 ggttatgtga tgatttagtc tgggatttta cttcagagaa acgggtgtct gtttagtcaa
      481 caaaataatc acaaacgcaa cggatgggtt ctcgacgtta ttcacgacag gctcgtacat
      541 tacagcgagt aataaaaaac ctgatatgca aagtcaaacc aatattcata atgtcttttt
      601 attcacaaca ataaataatt caataacaat cattgaaatg aatgatggtt tgtataagga
      661 atatacgcta ccgcccaccc tgataacatg atctgcacat tgccatctta acgtcgttaa
      721 acactggctt aatagtggat atttcaaatt aggaatttat taaattcacc tgatgaatat
      781 aataagcaaa tagcatagaa aataatggca actccccccg agaggaccat ttccaaagca
      841 ctgcaccacc acctggtgcc cggatgatct tgcttcagtg gtaagagtac ctgattcatt
      901 gttcccaaaa tgatgttttc cccggccccg ccaataatgt gcagctctca cgtgataat
//
LOCUS       DAZPDU010000425          936 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZPDU010000425 DAZPDU010000000
VERSION     DAZPDU010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.429"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(198..>936)
                     /locus_tag="ACHURS_004751"
     CDS             complement(198..>936)
                     /locus_tag="ACHURS_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR1240268.1"
                     /translation="GPTLTFLFVSPLLNPIIVGLMWMTFGWKVTLLYAIIAAGVSVLA
                     SIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIKVSCCSPAAKAIENPVVTCC
                     STKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFIYGFIPSAWIAAHAGADNPF
                     AIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALIIGSAGASLTEVILLKSMF
                     RMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT          280 a          198 c          199 g          259 t
ORIGIN      
        1 attgcttctc cgataaaatg tcagagaagc atgattacgt ggttattgtc tgttgatgac
       61 ttgttgaccg agccgtcatc attctcaatg cattagttaa atcatttgtg atttttaagg
      121 attatcctgt ctatgatttt ctacttagaa ggatatggca ctaactatgc atcttatagt
      181 ttactgctgg catctattta aaatagcaat tgagtcaaat agcccatcaa gatagccata
      241 cctaatataa ccgtcaggaa tgcagctatc atcggcattc tgaacattga tttaagtaat
      301 atcacttccg tcaggcttgc gccggcactg ccgatgatta atgccattaa tgctcccaga
      361 cccattcctt ttgtcatcag aacagatgcc agagggataa ccgcctcagc ccggatatac
      421 agcggaatac cgacaacggc gcttaatggg atggcgaagg gattatctgc tcctgcatga
      481 gcggcaatcc acgctgaggg aataaagcca taaataaaag atcctattaa tactcctaac
      541 aaaagatatg gcagtacatc tttaaactgt tgcaacgcat ctttggtggc tagttttatg
      601 ggattaatac ttactacagc tttggtactg caacaggtaa ctacggggtt ctcaatggct
      661 ttagccgctg gactacaaca actcactttt atgggcgtat atgtcgttcc cggcgaagtt
      721 tttgctggag cacaacaatt tgctgatgag cttttactgg caatgatatg acgttcaaac
      781 cccagagaat ccaggataat actggcaaga acggagacgc cggctgcgat aatcgcgtac
      841 aacaaggtga ctttccaacc aaaggtcatc cacattagtc cgacgataat gggattaagt
      901 aatggtgaaa caaataaaaa agtgagagtc ggacca
//
LOCUS       DAZPDU010000426          922 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZPDU010000426 DAZPDU010000000
VERSION     DAZPDU010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..922
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.430"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(342..>922)
                     /locus_tag="ACHURS_004752"
     CDS             complement(342..>922)
                     /locus_tag="ACHURS_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1240269.1"
                     /translation="RLYLAEMLYYPIDLIEKFQNLYAITDQIRNTTGFCLPQNSELIY
                     CWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSKQSLISRCYNLMLSEP
                     GTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNALSAIQTTVKPISEIA
                     RENGYKCPSRFTERFHNRFKITPRELRKASRE"
BASE COUNT          281 a          183 c          165 g          293 t
ORIGIN      
        1 ttaacgtata aactagattt aataataacg aactagattt aataacaacg atatccgtac
       61 ctatccggat atctaatgtc ataagcatcc ctttaattat cccctcaacc tcttgtaact
      121 ttcccactct tgaactccac atagtccaaa cgccggatgc aaaaaaatca cacgtgcatt
      181 tctgaaattg tgatggagga agatagtatc aattatatgt gcttataaac gtccccgatt
      241 agcgcagaag tcaccggatt gttcaggctc caataggttg agtatgccgt atttattttc
      301 caaaataaaa atcgatgctg ttgctccttc ataaaatact tttactctct ggacgctttt
      361 cttagctctc ttggtgttat cttaaaacga ttatggaacc tttcagtaaa acgagaagga
      421 cacttgtaac cattttccct ggcaatctcg ctgatgggtt ttactgtcgt ttgtatagca
      481 gacaacgcat tatttaacct cacgtcgtcc aatatacttt gaaaacttac tccctcgctt
      541 gccagacggc gatgcaatgt ggaaacagaa atgtagagat atctcgctac cttgtttgct
      601 gtccattttg ttccaggttc ggatagcatt aggttataac aacgacttat taatgattgt
      661 ttactatctg ataaaagtaa acaattaaca tgatgtgctc ctaatgaaag tagaacgccc
      721 attgctaagt gttcctgaat ttgcgttgag aagcctcggg aaatagatgt ttttagttgc
      781 tcccaacaat atattaactc ggaattctga ggtaagcaga aacctgtcgt gttacgaatt
      841 tgatcggtta tcgcataaag attttggaac ttttcaatta gatcaatggg ataataaagc
      901 atttctgcaa gataaagacg gt
//
LOCUS       DAZPDU010000427          921 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZPDU010000427 DAZPDU010000000
VERSION     DAZPDU010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..921
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.431"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..767)
                     /gene="ipaH3"
                     /locus_tag="ACHURS_004753"
     CDS             complement(<1..767)
                     /gene="ipaH3"
                     /locus_tag="ACHURS_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH3/Hc"
                     /protein_id="HFR1240270.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNHLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITYIPENILSLDPTCTIILEDNPLSSRIRESLSQQTA"
BASE COUNT          267 a          146 c          229 g          279 t
ORIGIN      
        1 tgggcggttt gttgcgacag agactccctg atccgtgagg acagaggatt gtcttcgagg
       61 atgatagtgc aggtcggatc aaggctaagt atattttccg gaatgtatgt gatgcgattc
      121 tcgcggcacc ggaaaaatat ctcggtttcc tctgagtgat gatttcttac aggtacggct
      181 ggtaggcttt ccagaagatt agtacttaca tcgagcgctt ccagtgattc aggtaactca
      241 gggagaaatg tcagctggtt atttcttact gagagcactt ccagcgatgt aggtaattca
      301 ggaagcatgg ttagctgatt gttatctgca ttaatatatt ccagcaatgc aggcaattca
      361 ggaagcatgg ttagttggtt gttatctaca tcaagatgtt tcagagatgc gggtaattca
      421 ggaagtgttg acaggtgatt gtcacaggcg tcaaggtatt ccagcgatgc tggcaattct
      481 ggtaatgata ttagggcatt ctgagtaatt tccagaacag tgatttgagg tggtaagttg
      541 tcaggtagcg aggacagatt taaacgattc agttgaagct cactgaactg attgatgaga
      601 cattctttaa gtagggagac cgcttcattc cgattttcac cggggagcgc ttgtttttcc
      661 catttatccc atgctgaaaa gtaatcagca tatgtaccgg aaatagtgtt ataaaaagaa
      721 ttttgggaca atgaaaagtt attatttatc ggtaacatga tggacattca gttaaagccg
      781 atggacaaat ggctttgtta aaagaaataa gtaggagttt ttatgaagtt cattccatga
      841 aatgaacttc agcacttttt tattataaaa ggacaaacaa aattggctgt aaaaaaacga
      901 tactgtttca gttaactgtg a
//
LOCUS       DAZPDU010000428          916 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZPDU010000428 DAZPDU010000000
VERSION     DAZPDU010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.432"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            53..838
                     /locus_tag="ACHURS_004754"
     CDS             53..838
                     /locus_tag="ACHURS_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240271.1"
                     /translation="MNLRRVCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     FQDTLQTGKYDSGLKSIVWSDYKNQKLTDTMSLRYLVRFTLVDVATGEWAAWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
BASE COUNT          302 a          172 c          202 g          240 t
ORIGIN      
        1 aatcgttagc taaacattac catgattatg acaggcgagg gagtgtccga ttatgaattt
       61 aagaagggtt tgttgtatat gtgtattatt ctcgctgctt gcaggatgtg cctctgaatc
      121 ttctattgat gaaaagaaga aaaaagctca agtcacacaa agtaatatta ataaaaacac
      181 tccccagcaa ctgacagaca aagatttatt cggtaatgaa accactctgg ccgtatccga
      241 agaggatatt caagctgcgc ttgatggaga tgagttccgc gttcccctta attctcctgt
      301 aatacttgtt caatccggca accgcgcacc ggaaaccatt atgcaggagg agatgcgaaa
      361 atattatact gtttccacat tctctggtat cccggacagg caaaagcctc tgacttgtaa
      421 caaaaacaaa gataaaaatg agaacgagga tgttgccagt gctgagaata tgaactggat
      481 gcaggcactg cgttttgtgg ctgcaaaagg gcatcagaaa gcgattattg ttttccagga
      541 tacgttgcag acaggaaaat atgactctgg attgaaatca atagtttggt cagattataa
      601 aaatcaaaaa ttgactgaca cgatgtcact gcgctatctg gtacgattca cgctggtgga
      661 tgtagctaca ggtgagtggg ctgcctggtc gccggtgaat tatgaatata aagtgcttcc
      721 accattgccc gacaagaatg aagccagtac gactgatatg acagagcagc aaatcatgca
      781 acttaagcaa aagacctata aagcgatggt aaaagatttg gttaaccgct atcaataata
      841 aattatatct gccgccagga atttctggcg gaaataatac aaaattcttt gcataagaaa
      901 tttttcttga caaaga
//
LOCUS       DAZPDU010000429          905 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZPDU010000429 DAZPDU010000000
VERSION     DAZPDU010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.433"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..796
                     /locus_tag="ACHURS_004755"
     CDS             <1..796
                     /locus_tag="ACHURS_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1240272.1"
                     /translation="HDNETELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVAS
                     DVNDPATTCTCALYGFQFQGAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQS
                     KNSQTSSLWDAFAYTLAILYYENFKNAYRSEQGYIKKDVLIKDILFYLNNNYREKITL
                     EQLSKKFRASVSYICHEFTKEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGY
                     ENVDHFAKLFLRHVGCSPSDYRRQFKNCFAEQEILSEFPQPVSLAG"
     gene            complement(839..>905)
                     /locus_tag="ACHURS_004756"
     CDS             complement(839..>905)
                     /locus_tag="ACHURS_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240273.1"
                     /translation="GTDEIMIYVAGRQILKDYQNK"
BASE COUNT          259 a          182 c          197 g          267 t
ORIGIN      
        1 tcatgacaat gaaaccgagt tgatttacgt caagaaaggg gtagcaaggt ttaccatcga
       61 ttcttcgtta tatgtcgcgc acgccgatga cattgtggtg atagaacgtg gcaggctgca
      121 tgcggtggcc tctgacgtta acgatccagc aacgacgtgt acctgtgcgc tgtacggctt
      181 tcagtttcag ggggctgagg aaaatcagct actgcaaccg cattcttgcc cggtaattgc
      241 cgcagggcag ggaaaagaag tcattaaaac cttatttaat gagctaagtg tgattttgcc
      301 gcaaagtaaa aatagccaaa catcttcgtt atgggatgca tttgcctata cattagcaat
      361 tctttactac gaaaacttta aaaatgctta tcgttcggag caggggtata ttaaaaaaga
      421 tgttctgata aaagatattc ttttctatct gaataataat tatcgcgaaa aaatcacttt
      481 agaacagtta tcgaaaaaat ttcgtgccag cgtcagttat atttgccatg aatttaccaa
      541 agagtatcgt atttccccta ttaactatgt tattcaacgg cgtatgacgg aagcaaaatg
      601 gtcactcact aatactgaat tatcacaggc agagatctcc tggcgtgtgg gttatgaaaa
      661 tgtcgatcac tttgccaaac tgtttttgcg ccatgtcggt tgttcgccca gcgattaccg
      721 taggcaattt aaaaattgtt ttgcggaaca agaaatctta tctgaatttc ctcaaccggt
      781 aagtcttgcc ggataaatct ggcggcgcac tttgttaaaa agctgcgccg cctgcagatt
      841 atttgttctg atagtctttc aggatctgcc gacctgctac gtaaatcata atttcgtctg
      901 taccg
//
LOCUS       DAZPDU010000430          890 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZPDU010000430 DAZPDU010000000
VERSION     DAZPDU010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.434"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..570)
                     /locus_tag="ACHURS_004757"
     CDS             complement(<1..570)
                     /locus_tag="ACHURS_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HFR1240274.1"
                     /translation="MDSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKQGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLA"
     gene            complement(570..>890)
                     /locus_tag="ACHURS_004758"
     CDS             complement(570..>890)
                     /locus_tag="ACHURS_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFR1240275.1"
                     /translation="AAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAGSVI
                     VTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGLVMMFYALCFINQSIQQLKQPA
                     QEAS"
BASE COUNT          297 a          211 c          194 g          188 t
ORIGIN      
        1 cgcaagaccc attaagatcc ctggcaaata tcccgccata aatagcgatg ctacagaaac
       61 gccgccagct gtcagggaaa acacaattaa cacgtttgag ggcggtatca gtaatccagt
      121 aatacaagat gagacgttta cagcggtaga aaatgccgga tcataacctt gtttggtctg
      181 aatgggattc agtgtgccac caacggcagc agctgccgca acggctgaac cagaaatgga
      241 accaaacatc atattcgcca ggacgttcac atgaccaagt gaaccaggaa cccgaccaac
      301 cattacctgg gcgaggttaa tcaaacgtat cgcaataccg ccgctgttca tcagcgttcc
      361 ggcaaagatg aaaaagggga ttgccaatag ggcaaagtta tccagtccat tagccagtcg
      421 ctgcgaaaca gtgattgccg cgatatccca gggaaacatc aaaagcattg aagctacagt
      481 ggcaataccg attgagaacg aaatgggcac accaataaag accagcacaa aaaaacagcc
      541 aaacagtgta atcgcgatat aactatccat taactggcct cctgcgcagg ttgtttcagt
      601 tgttgaatgc tttgattaat aaaacacaag gcataaaaca tcatcaccaa tccacttaat
      661 ggcagaatga tatatacgta tcccatcggt atttgcatcg cggcagatac ctgtgatgtt
      721 gccagcgttt tatcaattaa tttcaggcca ccagtcacaa tgaccgaacc agcaaaacct
      781 aaaataagaa cgttaatgac tatactaaga agtaattgct tgcgcttatt cagggcgaga
      841 gcaaataaat cgatagagag atgtctctgc gcgccgacgg tatatgcagc
//
LOCUS       DAZPDU010000431          881 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZPDU010000431 DAZPDU010000000
VERSION     DAZPDU010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.436"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            82..462
                     /locus_tag="ACHURS_004759"
     CDS             82..462
                     /locus_tag="ACHURS_004759"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1240276.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQQLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPKTLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
     gene            459..806
                     /gene="tnpB"
                     /locus_tag="ACHURS_004760"
     CDS             459..806
                     /gene="tnpB"
                     /locus_tag="ACHURS_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR1240277.1"
                     /translation="MIPLPSGIKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTSDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
BASE COUNT          219 a          239 c          228 g          195 t
ORIGIN      
        1 gcctgaaccg tctggtcaga atctgacgaa ttagacaaag tggtgtccac caaataagta
       61 gtgggaacca aagtgtcaga tatgcagaaa aatgtgactc ccggcaggcg aaaaggctgc
      121 cctaattatc ctcccgaatt taaacagcag ctcgttgctg cctcctgtga acccgggata
      181 tccatctcaa aacttgctct tgaaaatggc attaacgcca atctgttgtt caaatggcga
      241 caacaatggc gcgagggaaa gctgctatta ccttcttcag agagccccca gctacttcct
      301 gtgactctcg atgcagctgc cgaacagcca gaatcgctcg cagaggaccc gaaaaccctc
      361 agtatcagct gtgaggtaac gttccggcac gggacgctcc gcttcaatgg caatgtcagc
      421 gaaaagctcc tgactctgct gatacaggaa ctgaagcgat gatcccgtta ccttccggga
      481 tcaaaatttg gctggttgcc ggtatcaccg atatgagaaa tggcttcaac ggcctggctg
      541 cgaaagtaca gacggcgctg aaagacgatc ccatgtccgg ccatgttttc attttccggg
      601 gccgcagcgg cagtcaggtt aaactgctgt ggtccaccag tgacggactg tgcctcctga
      661 ccaaacggct ggagcgtggg cgcttcgcct ggccgtcagc ccgtgatggc aaagtgttcc
      721 ttacacaggc gcagctggcg atgctgctgg aaggtatcga ctggcgacag cctaagcggc
      781 tgctgacctc cctgaccatg ctgtaaatct ctttatcctg gttgtcacag aataagcccg
      841 gtaaaatacg ggcttatgaa cgacatctct tctgacgaca t
//
LOCUS       DAZPDU010000432          876 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZPDU010000432 DAZPDU010000000
VERSION     DAZPDU010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.437"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..>876
                     /gene="cadA"
                     /locus_tag="ACHURS_004761"
     CDS             <1..>876
                     /gene="cadA"
                     /locus_tag="ACHURS_004761"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFR1240278.1"
                     /translation="NFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVK
                     GDVNEETFNEAYMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEI
                     KRLRTESDGWFFDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPG
                     MEKDGTMSNFGIPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTD
                     FKRAFDLNLRVKNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVL
                     PTMVMTPYAAFQKELH"
BASE COUNT          238 a          220 c          212 g          206 t
ORIGIN      
        1 aacttctcac cgatttacga aggtaaatgc ggtatgagcg gtggccgtgt agaagggaaa
       61 gtgatttacg aaacccagtc cactcacaaa ctgctggcgg cgttctctca ggcttccatg
      121 atccacgtta aaggtgacgt aaacgaagaa acctttaacg aagcctacat gatgcacacc
      181 accacttctc ctcactacgg tatcgtggcg tccactgaaa ccgctgcggc gatgatgaaa
      241 ggcaatgcag gtaaacgttt gatcaacggt tccattgaac gtgcgatcaa attccgtaaa
      301 gagatcaaac gtctgagaac ggaatctgat ggctggttct ttgatgtatg gcagccggat
      361 catatcgata cgactgaatg ctggccgctg cgttctgaca gcacctggca cggcttcaaa
      421 aacatcgata acgagcacat gtatcttgac ccgatcaaag tcaccctgct gactccgggg
      481 atggaaaaag acggcaccat gagcaacttt ggtattccgg ccagcatcgt ggcgaaatac
      541 ctcgacgaac atggcatcgt tgttgagaaa accggtccgt ataacctgct gttcctgttc
      601 agcatcggta tcgataagac caaagcactg agcctgctgc gtgctctgac tgacttcaaa
      661 cgtgcgttcg acctgaacct gcgtgtgaaa aacatgctgc cgtctctgta tcgtgaagat
      721 cctgaattct atgaaaacat gcgtattcag gaactggctc agaatatcca caaactgatt
      781 gttcaccaca atctgccgga tctgatgtat cgcgcatttg aagtgctgcc gactatggta
      841 atgactccgt atgctgcgtt ccagaaagag ctgcac
//
LOCUS       DAZPDU010000433          869 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZPDU010000433 DAZPDU010000000
VERSION     DAZPDU010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..869
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.438"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..293)
                     /locus_tag="ACHURS_004762"
     CDS             complement(<1..293)
                     /locus_tag="ACHURS_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HFR1240279.1"
                     /translation="MNNCIFFSISASTVTARQFQRELDLADKAILWALISASTKEGRK
                     ACSLSYFACKAAEAELGLAYMAANDNKEFLTSLSNIMRYKIDAGLSESYTCYL"
     gene            complement(310..>869)
                     /locus_tag="ACHURS_004763"
     CDS             complement(310..>869)
                     /locus_tag="ACHURS_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFR1240280.1"
                     /translation="RCKTRWTRCTRRREKIGLKGGWNFYQYPLNPITDIDPLGLATCL
                     YSITLSMLSCVSDTQNDDNSYDVLTIPVASGNNGNNMQCKNNPGCTHLQNRGPIPQGV
                     WSWNVNGPGATNRKPNGIRLVPSANTETYNRDGFLTQSCLNAFGPSLGPRFCSEGCIT
                     GSSNDMQKLNELIFSEPDSTLTVTD"
BASE COUNT          255 a          182 c          152 g          280 t
ORIGIN      
        1 aataaatagc atgtatagga ttcggaaagt ccagcatcta ttttgtacct cattatatta
       61 gataaggatg tgagaaattc cttgttatca ttcgcagcca tatatgccag tccgagttca
      121 gcctctgcgg ccttacaggc aaaatagctt agtgaacacg cttttcgtcc ctctttagta
      181 ctggctgata ttagtgccca taatattgct ttgtctgcca aatctaactc tctctgaaat
      241 tgtcttgcag taactgtact cgcagaaata gagaaaaaaa tacaattatt aataactttg
      301 ccatattttt tagtccgtaa ctgttaatgt gctatctggc tctgaaaata ttaactcatt
      361 taatttttgc atgtcattag atgaaccagt tatacaaccc tcggaacaga atcgaggtcc
      421 taaagacgga ccgaaagcgt ttaaacaact ttgtgttaaa aatccatcac gattatatgt
      481 ttctgtattt gctgacggta ctaaacgtat accattaggt tttctattgg ttgctccagg
      541 accattaaca ttccagctcc ataccccttg aggtattgga cctctatttt gtaagtgtgt
      601 acaaccagga ttatttttac attgcatatt attaccatta ttaccagagg cgacaggaat
      661 agtaagcaca tcataagagt tatcatcatt ttgcgtatct gaaacgcatg aaagcatact
      721 taaagttatg ctgtataaac aggtcgctaa tcccaacggg tcaatatcag ttatcggatt
      781 caacggatac tgataaaaat tccatccccc cttcagtcca atcttttcgc gccggcgtgt
      841 acaccgggtc catctggttt tgcatctgc
//
LOCUS       DAZPDU010000434          868 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZPDU010000434 DAZPDU010000000
VERSION     DAZPDU010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..868
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.439"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            69..>868
                     /locus_tag="ACHURS_004764"
     CDS             69..>868
                     /locus_tag="ACHURS_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFR1240281.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEIYNKNGNKLDLYGKVDARHTF
                     SDNPGDDGDETIIELGFKGETQITDQLTGYGQALTKTKASDTEGSDNTYVKLAFAGLK
                     FGEMGSFDYGRNYGVIYDVEAWTDMLPVFGGDSYTWTDNFMAGRANGVATYRNSDFFG
                     LVEGLNFALQYQGNNEGSNAGEDQEGTKNGHEDVRFQNGDGFGLSTSYDFDFGLSLGA
                     AYSNSDRTNSQVALGGYHYNEYSKFAGGDTAEAWTFGAKYDANNVYLA"
BASE COUNT          249 a          167 c          207 g          245 t
ORIGIN      
        1 tgcttttttt ttggataacg gcacttattg atatattcat gaagattata atcacaaggg
       61 aatacataat gaaaagaaaa gttctggcaa tgctggtccc agcgttatta gttgctggcg
      121 cagcaaatgc ggctgaaatt tataataaaa atggcaataa actggatctg tacggaaaag
      181 ttgatgctcg tcatactttt tccgataacc ctggcgatga cggcgatgaa actatcatcg
      241 agttaggctt caaaggtgag acccaaatta cagatcagtt aactggttat ggtcaagcac
      301 tgaccaaaac aaaggctagc gacactgaag gttctgataa tacttatgtc aaacttgctt
      361 ttgcaggtct gaagttcggc gagatgggct cctttgacta tggtcgtaac tatggtgtga
      421 tttacgacgt tgaagcctgg acagatatgc ttcctgtatt cggcggcgac tcctatacct
      481 ggactgataa cttcatggca ggccgtgcaa atggggtagc tacttaccgc aatagtgatt
      541 tcttcggtct ggtggaaggt ctgaactttg cattgcagta tcaaggtaac aacgaaggca
      601 gcaatgcagg agaagatcaa gaaggtacta aaaatggtca cgaagacgtt cgcttccaga
      661 acggtgatgg ttttggtctt tccacttcat atgactttga cttcggtttg agcttaggtg
      721 ccgcttactc aaactctgac cgtaccaatt ctcaagtagc tcttggcggg tatcactaca
      781 atgaatacag caaatttgct ggtggtgata cagctgaagc atggacattt ggtgcaaaat
      841 acgacgccaa caatgtttat cttgcaat
//
LOCUS       DAZPDU010000435          865 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZPDU010000435 DAZPDU010000000
VERSION     DAZPDU010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..865
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.440"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..580
                     /locus_tag="ACHURS_004765"
     CDS             <1..580
                     /locus_tag="ACHURS_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR1240282.1"
                     /translation="LARLQRQLSRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVT
                     TAVSKNHAMIVIEDLKVSNMSKSAAGTVSQPGRNVRAKSGLNRSILDQGWYEMHRQLE
                     YKQLWRGGQVLAVPPAYTSQRCAYCGHTAKENRLSQSKFRCQVCGYTANADVNGARNI
                     LAAGHAVLACGEMVQSGRPLKQEPTEMIQATA"
     gene            complement(620..>865)
                     /locus_tag="ACHURS_004766"
     CDS             complement(620..>865)
                     /locus_tag="ACHURS_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042925 - benzoate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042919 - benzoate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HFR1240283.1"
                     /translation="AALPVSWIQMLAGLALLSTIGGSLYQALHNERERERDAAVVAFL
                     VTASGLTLVGIGSAFWGLIAGGVCYVVLNLIADRNRY"
BASE COUNT          237 a          241 c          226 g          161 t
ORIGIN      
        1 gctggcgaga cttcagcgac agttaagccg caaggtcaaa ttcagcaaca actggcagaa
       61 gcagaaacgc aaaatacagc gactgcattc ctgtatcgca aatatccgca gggactacct
      121 tcacaaagtc acaacggccg tcagcaaaaa ccacgcaatg atagtcattg aggatttgaa
      181 ggtcagcaac atgtcaaagt cagcagcggg tacggtcagc cagccggggc gcaatgtccg
      241 ggcaaaatca ggtttaaacc gttcgatact ggatcagggc tggtatgaaa tgcaccgcca
      301 gcttgagtac aagcagctct ggcgtggcgg tcaggtgctt gctgttccgc cagcgtatac
      361 aagccagcgt tgcgcgtact gtggtcatac agcgaaagag aaccgcctgt cacaaagtaa
      421 attcagatgc caggtatgtg gatatacagc gaacgccgat gtaaatggtg ctcgtaacat
      481 tttagcggcg gggcacgccg ttcttgcctg tggagagatg gtgcagtcag gccgcccgtt
      541 gaagcaggaa cccaccgaaa tgattcaggc gacagcctga acgtagcagg gatccacgtc
      601 cttcagggcg tggaggatgt caatatcggt ttctgtcagc gattaaattc aacaccacgt
      661 aacaaacgcc tccggcaatt aatccccaga acgcagaacc aatcccgacc agcgtcaatc
      721 cacttgccgt taccagaaat gccaccaccg ccgcgtcgcg ctcacgctca cgctcattat
      781 gcagcgcctg atacaaactg ccgccaatgg tactcaacag cgccagacct gccagcatct
      841 ggatccaact tacgggcagg gcagc
//
LOCUS       DAZPDU010000436          862 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZPDU010000436 DAZPDU010000000
VERSION     DAZPDU010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..862
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.441"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..343
                     /locus_tag="ACHURS_004767"
     CDS             <1..343
                     /locus_tag="ACHURS_004767"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFR1240284.1"
                     /translation="ADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVAHGELGGDFSLV
                     PDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASVLQLHPSLIVIA
                     DKAAAAELALG"
     gene            complement(405..776)
                     /locus_tag="ACHURS_004768"
                     /pseudo
     CDS             complement(405..776)
                     /locus_tag="ACHURS_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CbrC family protein"
     gene            complement(794..>862)
                     /locus_tag="ACHURS_004769"
     CDS             complement(794..>862)
                     /locus_tag="ACHURS_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR1240285.1"
                     /translation="FSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT          234 a          207 c          192 g          229 t
ORIGIN      
        1 tgcagatggt catttttgtg gcaatttgcc gaatacgacc catttccatg agcaaaccgt
       61 ggagttcccg atccagggag agatggttga tattgtggcg catggtgagt tgggcggcga
      121 cttttcgtta gtgcctgaca gctacgtgac gatggggccg aaaagcatca tggcggcgaa
      181 aaacttgctg ataattgtca gtggcgcagg taaagcgcag gcactgaaaa atgtgctgca
      241 aggtccggtt accgaagatg ttccagcttc cgtgttgcag ctgcatcctt cgctgatagt
      301 aatcgctgat aaagccgctg ccgcagaatt agcgctcggt taagctgaag aaataaggcc
      361 ggatgtatga attacgctcc ggcctgttct cattatttaa ataactacga aaaatcaccc
      421 cacagtctca gcttgccgca gtggagacag cggaagagat aaccctgaca atcaccaccc
      481 ttttgcaggc atttagctag atcagaattt ctaataccga aattctcaca atcttcttca
      541 aggttggcaa attcatcgag gcgatctttt atatcattcc agcccacata gccgataaaa
      601 gcacagaaat cgccacaatg cgcaagccag aattcctgtt gccatccatg ataacctggc
      661 gtgcgttcaa ccaactcttt cagcatttca tcaggatatg tgttcttaat accggcaaat
      721 tcgtcctctt catcatactc aaattcaaca ccttctatgc tggcatcatc ctgaaaacgg
      781 taatgactcc aacttattga tagtgtttta tgttcagata atgcccgatg actttgtcat
      841 gcagctccac cgattttgag aa
//
LOCUS       DAZPDU010000437          842 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZPDU010000437 DAZPDU010000000
VERSION     DAZPDU010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.442"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            54..130
                     /locus_tag="ACHURS_004770"
     tRNA            54..130
                     /locus_tag="ACHURS_004770"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:88..90,aa:Ile,seq:gat)
     gene            173..248
                     /locus_tag="ACHURS_004771"
     tRNA            173..248
                     /locus_tag="ACHURS_004771"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:206..208,aa:Ala,seq:tgc)
     gene            432..>842
                     /locus_tag="ACHURS_004772"
     rRNA            432..>842
                     /locus_tag="ACHURS_004772"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          238 a          177 c          232 g          195 t
ORIGIN      
        1 ctttgcagtg ctcacacaga ttgtctgatg aaaatgagca gtaaaacctc tacaggcttg
       61 tagctcaggt ggttagagcg cacccctgat aagggtgagg tcggtggttc aagtccactc
      121 aggcctacca aatttgcatc gcaaatttga agaggtttta actacatgtt atggggctat
      181 agctcagctg ggagagcgcc tgctttgcac gcaggaggtc tgcggttcga tcccgcatag
      241 ctccaccatc tctgtagtga ttaaataaaa aatacttcag agtgtacctg caaaggttca
      301 ctgcgaagtt ttgctcttta aaaatctgga tcaagctgaa aattgaaaca ctgaacaatg
      361 aaagttgttc gtgagtctct caaattttcg caacacgatg atgaatcgca agaaacatct
      421 tcgggttgtg aggttaagcg actaagcgta cacggtggat gccctggcag tcagaggcga
      481 tgaaggacgt gctaatctgc gataagcgtc ggtaaggtga tatgaaccgt tataaccggc
      541 gatttccgaa tggggaaacc cagtgtgatt cgtcacacta tcattaactg aatccatagg
      601 ttaatgaggc gaaccggggg aactgaaaca tctaagtacc ccgaggaaaa gaaatcaacc
      661 gagattcccc cagtagcggc gagcgaacgg ggagcagccc agagcctgaa tcagtgtgtg
      721 tgttagtgga agcgtctgga aaggcgcgcg atacagggtg acagccccgt acacaaaaat
      781 gcacatattg tgagctcgat gagtagggcg ggacacgtgg tatcctgtct gaatatgggg
      841 gg
//
LOCUS       DAZPDU010000438          838 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZPDU010000438 DAZPDU010000000
VERSION     DAZPDU010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.443"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..524
                     /locus_tag="ACHURS_004773"
     CDS             <1..524
                     /locus_tag="ACHURS_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFR1240286.1"
                     /translation="SLARQYGYDESTVRTWVEKGMPTDTDSNARSWIVDNVLKPLRDT
                     NTKEQIEQERLKKLSAERQLAELELAEKNGLVVSTEYIEQVLTEYLFQVKTAVRAIPS
                     KTYLELFAQSDAKDLRDVLRTHIDKTLYQLGSMEFELPDDMEVIGNKQEEINEDTEES
                     TTDDTTAEDTENE"
     gene            568..>838
                     /locus_tag="ACHURS_004774"
     CDS             568..>838
                     /locus_tag="ACHURS_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015690157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFR1240287.1"
                     /translation="MGLDWVPFSFQKEPMDIAQERSTKKIVLQSCSQLLKTTVLQSIA
                     FNLMANDPCNFAFGSSSESEVKKFKDGKFLPAIETSSVLKPLVTDK"
BASE COUNT          307 a          135 c          184 g          212 t
ORIGIN      
        1 ggtcattagc gagacagtac ggatacgatg aatctacagt tcgaacttgg gtagaaaagg
       61 gaatgccgac agacactgat tcaaacgcaa gaagttggat tgtagataat gtactaaagc
      121 cgttgcgtga tactaataca aaagaacaaa ttgaacaaga gcgtttgaag aagctttcag
      181 cagaacggca gttagcagaa ttagaattag cagaaaagaa tgggctggtg gtcagtactg
      241 aatatataga acaggtacta acggaatatc tattccaggt caaaacagca gtgcgtgcaa
      301 taccatcaaa aacttatctt gaattatttg cacaatcaga tgcaaaggat ttacgcgatg
      361 tattaagaac gcatattgat aaaacattat accaattagg ctcaatggaa tttgagttac
      421 ccgatgacat ggaagtaata ggaaacaaac aagaagaaat taacgaggat actgaagaaa
      481 gtactaccga cgatacaacc gccgaagata cagaaaacga gtgagtggat tagtaatggt
      541 gtagttaaat ttgtggacgg gccgaatatg ggccttgatt gggttccgtt tagtttccaa
      601 aaagaaccaa tggatatagc tcaagagcga agtactaaaa agattgtact tcaatcttgt
      661 tcccagcttt tgaaaactac agtactacaa tcaattgcat ttaacctaat ggctaatgat
      721 ccctgtaact ttgcttttgg tagtagttct gaatcagaag tgaagaaatt caaggatggt
      781 aaatttcttc cagctattga aaccagttca gtactaaagc cgttagtaac cgataaga
//
LOCUS       DAZPDU010000439          837 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZPDU010000439 DAZPDU010000000
VERSION     DAZPDU010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..837
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.444"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..725
                     /gene="ydeO"
                     /locus_tag="ACHURS_004775"
     CDS             <1..725
                     /gene="ydeO"
                     /locus_tag="ACHURS_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFR1240288.1"
                     /translation="FNANILDKDYAFSDGEILMVDNAVRTHFEPYERHFKEIGFTENT
                     IKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERNHHNFSELLLFSCL
                     SIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCLYISESLLKKKLKQ
                     EQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYAFRKHFGNSPKRVS
                     KEYRCQSHMGMNTGNTMSALAI"
BASE COUNT          256 a          165 c          170 g          246 t
ORIGIN      
        1 ccttcaacgc taacatttta gataaagatt acgccttctc tgacggcgag atcctgatgg
       61 tagataacgc tgttcgtacg cattttgaac cttatgagcg gcattttaaa gagatcggat
      121 ttactgaaaa taccattaaa aaatatctac aatgcactaa catccagaca gtgacggtgc
      181 ctgttcctgc gaagttttta cgtgcttcaa atgtaccgac tggattgctt aatgaaatga
      241 ttgcttatct taactcggaa gaacgcaatc atcataattt ttcagaactt ttgctttttt
      301 cttgcctgtc tatttttgcc gcatgcaaag gtttcattac actattaact aacggtgtgc
      361 tatccgtttc cggaaaagtg agaaatattg tcaacatgaa gctggcgcac ccatggaagc
      421 tgaaagatat ttgtgactgc ctgtacatca gtgaaagcct gttgaagaaa aaactaaagc
      481 aagagcaaac gacattctca cagattctat tagatgccag aatgcagcac gcaaaaaatt
      541 tgatacgcgt agaaggttca gtcaataaaa ttgccgaaca atgtggttat gccagtacat
      601 cttattttat ttatgcgttc cgcaaacatt tcggcaacag tccgaagaga gtttctaagg
      661 agtaccgttg tcaaagtcac atgggtatga atacgggcaa cacgatgagt gctttagcta
      721 tttgattatt tgctaacgag tagtcaacca cgcacgctgc gtaagaatta aatggggcag
      781 ccattccctg ccccgcgttg tttttaggcg atatatttat tgaaataaat aagtgac
//
LOCUS       DAZPDU010000440          833 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZPDU010000440 DAZPDU010000000
VERSION     DAZPDU010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.445"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..>833
                     /locus_tag="ACHURS_004776"
     CDS             <1..>833
                     /locus_tag="ACHURS_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFR1240289.1"
                     /translation="LQSQHLNSLLSDRDYTWNDNGELIRISSPRQTRSYSYSTTGRLT
                     GVHTTAANLDIRIPYATDPAGNRLPDPELHPDSTLSMWPDNRIARDAHYLYRYDHHGR
                     LTEKTDLIPEGVIRTDDERTHRYHYDSQHRLVHYTRTQYAEPLVESRYLYDPLGRRVA
                     KRVWRRERDLTGWMSLSRKPQVTWYGWDGDRLTTIQNDRTRIQTIYQPGSFTPLIRVE
                     TATGELAKTQRRSLADALQQSGGEDGGSVVFPPVLVQMLDRLESEILADRVSEESRRW
                     L"
BASE COUNT          189 a          266 c          254 g          124 t
ORIGIN      
        1 gttacagagc cagcacctga acagcctgct gtctgaccgc gattacacct ggaacgacaa
       61 cggcgaactc atccgcatca gcagcccgcg ccagacccgg agttacagct acagcaccac
      121 cggcaggctg accggcgttc acaccaccgc agcgaatctg gatatccgca tcccgtatgc
      181 cacagacccg gcggggaacc gcctgcccga cccggagctg cacccggaca gcaccctcag
      241 catgtggccg gataaccgta tcgcccgtga cgcgcactat ctttaccggt atgaccatca
      301 cggcagactg acagagaaaa ccgacctcat cccggaaggg gttatccgca cggatgatga
      361 gcgcacccat cggtaccatt acgacagtca gcaccggctg gtgcactaca cgcggacaca
      421 atatgcagag ccgctggtcg aaagtcgcta tctttacgac ccgctgggcc gcagggtggc
      481 aaaacgggta tggcggcgtg aacgggacct gacgggctgg atgtcgctgt cacggaaacc
      541 gcaagtgacc tggtacggct gggacggcga ccggctgacc acaatacaga acgacagaac
      601 ccgcatccag acgatttatc agccggggag cttcacgcca ctcatcaggg tcgaaactgc
      661 caccggtgag ctggcgaaaa cgcagcgccg cagcctggcg gatgcccttc agcagtccgg
      721 cggcgaagac ggtggcagtg tggtgttccc gccggtgctg gtgcagatgc tcgaccggct
      781 ggaaagtgaa atcctggctg accgggtgag tgaggaaagc cgccgctggc tgg
//
LOCUS       DAZPDU010000441          821 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZPDU010000441 DAZPDU010000000
VERSION     DAZPDU010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..821
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.446"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..95)
                     /locus_tag="ACHURS_004777"
     CDS             complement(<1..95)
                     /locus_tag="ACHURS_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240290.1"
                     /translation="MRLHVKLKEFLSMFFMAILFFPAFNASLFFTG"
     gene            complement(105..>821)
                     /locus_tag="ACHURS_004778"
     CDS             complement(105..>821)
                     /locus_tag="ACHURS_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240291.1"
                     /translation="ILSPITSFDYIHFITTHPSGIKDTLAWLVNASKLMTEFDDNGKI
                     IFNINALKYTKASYFEILGEKYIKITTSSPWLLEKLGKYIFSSRAPQVLELAIGWRGA
                     LSESIKGVKFCIWFSVAWRTIEFIMSSERDLVNFLGDFSMDVAKAVIAGGVATAIGSL
                     ASFACVSFGFPVILVGGAILLTGIVCTVVLNEIDAQCHLSEKLKYAIRDGLKRQQELD
                     KWKRENMTPFMYVLNTPPVI"
BASE COUNT          271 a          160 c          140 g          250 t
ORIGIN      
        1 ccggtaaaaa acaaagaagc attgaatgca gggaaaaata atatggccat aaaaaacatc
       61 gaaagaaact cttttaattt aacatgtaaa cgcatggtta atcctcatat cacgggtgga
      121 gtgttaagaa catacataaa tggagtcatg ttttcccttt tccatttatc aagttcctgt
      181 tgccgtttta gtccatctct aattgcatat tttaattttt ctgataaatg gcattgagca
      241 tcgatttcat ttaaaacaac tgtacacact atccctgtca gtaaaattgc tcctccgaca
      301 agaattactg gaaagccaaa gctaacacag gcaaaagaag ccaggcttcc aatagcggtt
      361 gcaacaccac cggctataac tgctttggcc acatccattg aaaaatcgcc aagaaaatta
      421 accagatctc gctctgaact cataataaat tctatggttc tccaggctac tgaaaaccag
      481 atacagaact taactccttt tattgattca gataaagctc ctcgccagcc aatagcgagt
      541 tccagaactt gaggcgctcg tgaagagaaa atatatttac cgagcttttc taaaagccag
      601 gggctggatg ttgtgatttt tatatatttc tctccaagta tttcaaagta acttgctttt
      661 gtatacttta aggcattaat gttaaatata attttcccat tgtcatcaaa ctcagtcatt
      721 aatttactcg cgttaactaa ccaggcgagt gtatctttta tacctgaagg atgtgtagtg
      781 ataaaatgaa tgtaatcaaa tgatgttatt gggctgagta t
//
LOCUS       DAZPDU010000442          815 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZPDU010000442 DAZPDU010000000
VERSION     DAZPDU010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.447"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(246..>815)
                     /locus_tag="ACHURS_004779"
     CDS             complement(246..>815)
                     /locus_tag="ACHURS_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1240292.1"
                     /translation="FFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIV
                     IAGILLNVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFF
                     IAVVLGMIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQ
                     IHLLDKYRKVPPEPVHADIPVGAVSDVKA"
BASE COUNT          259 a          203 c          174 g          179 t
ORIGIN      
        1 agggacagct gatagaaaca gaagccactg gagcacctca aaaacaccat catacactaa
       61 atcagtaagt tggcagcatc acccgctttg gcttgataac cggataacaa cgtgcgcttt
      121 gttgatgccg gatgcggcgt gaattcctta tccggtctac aaaatttgca aatccaaagc
      181 attacattaa gacgtggcct gataaacata tcgcatcagg ccattttcgt tagctattgt
      241 ccacgtcagg ctttcacatc actcactgca ccgaccggaa tatcagcatg aacaggctcg
      301 ggcggtactt tgcgatattt atccagcaga tgaatctgga tcctgcgcag cgtgtcgcca
      361 ttgaggcgat agaaacggaa gtagagaatc agcgttacca tgaaaaacag agttggtaga
      421 gcaatcataa taaactgcat acctaatagg gcttgcgtag actgttcaac attcggtaca
      481 tagccaatca tccctaacac aaccgcaatg aaaaaagccg caaaggctga accgcccttc
      541 accaccatag tctgcacgga gtaagcgata ctttcacagc gtacgtgcag tttatattca
      601 ccgtaatcaa cggtatctgc caccatgatg acctgtaata cccagaaaag cgccgttccc
      661 acattcagca aaatacccgc aatcacaatg aggacgacgt tgtgatagct cattaatgcc
      721 attaacagga gaacaccaca gcttaacacc ggaagaatag atgctccggc ccataaaatg
      781 cgtcgggata atgatttaac taagcgtggg aagaa
//
LOCUS       DAZPDU010000443          809 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZPDU010000443 DAZPDU010000000
VERSION     DAZPDU010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..809
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.448"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..735
                     /locus_tag="ACHURS_004780"
     CDS             <1..735
                     /locus_tag="ACHURS_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFR1240293.1"
                     /translation="PKYFADLPTSQFWSYATYFRVLSFEYLSESISTLLYLDADVVCK
                     GSLKPLTEIIFKDEFAAVIPDNVSTQAACAKRLNIPEMNGRYFNAGVIYVNLKKWHEA
                     NLTPYLLTLLRGETKYGSLKYLDQDALNIAFNMNNIYLAKDFDTIYTLKNELHDRSHR
                     KYQQTITDKTVLIHYTGITKPWHSWAGYPSASYFNIAREQSPWKKYPLKEARTVAEMQ
                     KQYKHLFAHGEYIKGITSLIKYKLKK"
BASE COUNT          261 a          153 c          152 g          243 t
ORIGIN      
        1 ccaaaatact ttgctgatct gccgacgtca cagttttggt cgtacgcgac atacttcagg
       61 gtattgtcct ttgaatatct gagtgaaagt atttccacac tgctgtatct ggatgccgat
      121 gtcgtttgta aaggaagtct gaagcctctc acagaaatta tatttaaaga tgagtttgct
      181 gcggtcattc ctgacaatgt tagtactcag gcggcatgtg caaaacgcct caacattccc
      241 gagatgaatg gacgttattt caatgcaggc gttatctatg tcaatcttaa aaaatggcat
      301 gaagcaaatt tgacaccgta tttactcacg cttttacgag gggaaactaa atatggctct
      361 cttaaatatt tagatcagga tgcgttgaat atcgcattta atatgaataa tatctacctc
      421 gcgaaggatt ttgatactat ttataccctg aaaaacgaac ttcatgatcg tagtcatcga
      481 aagtatcagc aaaccattac cgataaaaca gtattgattc actatacagg gataactaaa
      541 ccatggcata gctgggctgg ttatccgtct gcatcatact ttaatatcgc gcgtgaacaa
      601 tctccctgga agaaatatcc tcttaaagag gcgcggactg ttgcagaaat gcagaaacaa
      661 tataagcatc tgtttgccca tggtgagtat attaaaggca taacttcatt aattaagtac
      721 aagcttaaga aataaattac tcttatgatt acaagtatac gctatcgcgg cttctcattt
      781 tattacaaag ataatgataa taaatataa
//
LOCUS       DAZPDU010000444          802 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZPDU010000444 DAZPDU010000000
VERSION     DAZPDU010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.449"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            9..802
                     /locus_tag="ACHURS_004781"
                     /pseudo
     CDS             9..802
                     /locus_tag="ACHURS_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238933.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT          247 a          160 c          220 g          175 t
ORIGIN      
        1 acacgtaggg tgaagccggg ctgaagcggg tggtgaaaaa ggaacacgcg gacggcagcg
       61 tcacgcagag tcagtttgac gccgtgggca ggctcaggac acagacggat gccgcaggcc
      121 ggacaacaga atacagcccg gatgtggtga cgggtctcat cacgcgcatc accacgccgg
      181 atggcagggc atcggcgttt tactataacc accacagcca gttaacgtca gccaccgggc
      241 ctgacgggct ggaaatacgc cgggaatatg atgaatgggg caacctgctg aatgaagaga
      301 acccgcatca gctgcagcag cttattcgtt tgccggggca gcagtatgat gaggagtccg
      361 gcctgtatta taaccgccac cgctattatg acccgctgca ggggcggtat atcactcagg
      421 atccgattgg gctgaagggg ggatggaacc tgtatacata tccgctgagc ccggtgaata
      481 gcatggatcc attaggatta tatgaattta aatcaaaaaa tattgatgat attggaatat
      541 ttgcattggc aatgtgtaat ggagaatcaa ttaacgagaa taaagaatat ggtggactaa
      601 tatgtaagaa gcaaggtgaa tatttcccca tgaatccgat aagttcaaat gataatgata
      661 gtgtagactt gcgaaatata aaatgccctg aaggttcaga gagagtaggc gattatcaca
      721 ctcacggttt ttactctgac gataaaggaa ataaagtaac aaaagaaaat gatgtttatg
      781 atagtctaaa tttttcaagc aa
//
LOCUS       DAZPDU010000445          802 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZPDU010000445 DAZPDU010000000
VERSION     DAZPDU010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.450"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..228)
                     /locus_tag="ACHURS_004782"
     CDS             complement(<1..228)
                     /locus_tag="ACHURS_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HFR1240294.1"
                     /translation="MNNILLPPINIPCTLFETISLFDDFSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASS"
     gene            409..561
                     /locus_tag="ACHURS_004783"
                     /pseudo
     CDS             409..561
                     /locus_tag="ACHURS_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
     gene            complement(586..>802)
                     /locus_tag="ACHURS_004784"
     CDS             complement(586..>802)
                     /locus_tag="ACHURS_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240295.1"
                     /translation="NIGAKRIRDTGCKAIYFVLISLGLTLFSIISAAIEGVRCESLSV
                     QLNVAWILFTTIMLSAIPSAKMSLSAH"
BASE COUNT          254 a          146 c          157 g          245 t
ORIGIN      
        1 acttgaggca ctaaaagcaa cattaataga acctgggcca gggaaatggt atttaataag
       61 tctgtaagga tcaacttttg ctgagatatc gcttaggccg agtgagagaa agtcttgttc
      121 taccatatca ccatattgca tatcatcagc agaaaaatca tcgaataaac taatagtttc
      181 gaaaagtgta cagggaatat taattggcgg aagtaatatg ttattcattt gcatttgcct
      241 tggttatatt taagctcaag atcaattcga gaacaaatgc tagggtaaaa aaatatttaa
      301 aaaagaagta cacttcacat tttttcatta catctctgaa gagaacctcg ttaatgattg
      361 gtcttgctca ttgttaataa caaaatgttg aatttaccga ttaggaaact ttttcctacg
      421 cggattgcct ccagctatgg catgaacttt gagtttatgc cagaactgaa gtggagcttt
      481 ggctaccctg gcgcgattat ctttatgatc cttgcgggcc tgacaccgta tctgtacttt
      541 aagcgtaaga actggctgta actatacgat gtcgctgtga gtaagttagt gtgctgacag
      601 cgacatttta gccgagggaa tcgcagataa cataattgtt gtgaataata tccatgccac
      661 gttgagttgg acgcttaatg actcacatct caccccttca attgctgcag agataataga
      721 aaaaagtgtc aaacccaatg aaatcaatac aaagtagatt gccttacatc cagtatctct
      781 tattcttttt gctccaatat tg
//
LOCUS       DAZPDU010000446          799 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZPDU010000446 DAZPDU010000000
VERSION     DAZPDU010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..799
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.451"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..741)
                     /locus_tag="ACHURS_004785"
     CDS             complement(<1..741)
                     /locus_tag="ACHURS_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFR1240296.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKEL
                     THKLESIFNNTQVQIAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAQGLELAINALNNQLTSQHQY
                     PTNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIYLLDL
                     FLFSDKIFSIAVFSFFFTFTIFMVFTCLC"
BASE COUNT          268 a          137 c          161 g          233 t
ORIGIN      
        1 acataaacag gtaaagacca taaatatggt gaaagtaaaa aagaaggaaa atacagcaat
       61 ggaaaaaatt ttatcggaga acaggaataa atctaaaaga tagattgctg aaatacagac
      121 tccactttta catgcgcgac aaaaattact gccttgatga aaaaaccaga aagggaagaa
      181 catcactgca aatacccaaa aaatagcaaa gtaataatga tcactggaag acgctgattc
      241 actttctgaa ggattagtcg gatattggtg ttgtgaagtg agttggttat tcaaagcgtt
      301 tatagctagc tctaatccct gagctaattt ttgttgttta aatgcgggta tcatgttgct
      361 acggatgata tctccagcca gagcatcggt taccttttct tccagcccat aacctacctg
      421 gatgcggaca gtacgatctg accaggcaac aatgatcagt atcccatcat tacgtttggc
      481 atctcctaaa cgccaattgt caaaaactct tgtagcatat tgttcaattg tctcgtcttt
      541 ggttgtggga acaattaata cagcaatttg cacttgggta ttattgaaaa tagattctaa
      601 cttgtgcgtc agttctttct tttcttgtga gtttaagcta ctcgtaagat ccgtaacttg
      661 ttgctgtaat tgtgggacaa tagttggttg ggctgcaaca aaggaacaca cgaaagtgaa
      721 cattagtatg aaatagcgca ttataaattt ctcattaaat ctggataaat aaattgacgg
      781 cggcatcggt aattttaga
//
LOCUS       DAZPDU010000447          790 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZPDU010000447 DAZPDU010000000
VERSION     DAZPDU010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.452"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            52..790
                     /locus_tag="ACHURS_004786"
                     /pseudo
     CDS             52..790
                     /locus_tag="ACHURS_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
BASE COUNT          228 a          194 c          208 g          160 t
ORIGIN      
        1 gccacctgaa cagaggtgat atgctcacct cagaacaaca caggtgctcc aatgaaaaaa
       61 agaaatttta gcgcagagtt taaacgcgaa tccgctcaac tggttgttga ccagaaatac
      121 acggtggcag atgccgccaa agctatggat gttggccttt ccacaatgac aagatgggtc
      181 aaacaactgc gtgatgagcg tcagggcaaa acaccaaaag cctctccgat aacaccagaa
      241 caaatcgaaa tacgtaagct gaggaaaaag ctacaacgca ttgaaatgga gaatgaaata
      301 ttaaaaaagg ctaccgcgct cttgatgtca gactccctga acagttctcg ataatcggga
      361 aactcagagc gcattatcct gtggtcacac tctgccatgt gttcggggtt catcgcagca
      421 gctacagata ctggaaaaac cgtcctgaaa aaccagacgg cagacgggct gtattacgca
      481 gtcaggtact tgagctacat ggcatcagcc acggttcggc cggagcaaga agcatcgcca
      541 caatggcaac ccggagaggc taccagatgg gacgctggct tgctggcagg ctcatgaaag
      601 agctggggct ggtcagctgt cagcagccga ctcaccggta taaacgtggt ggtcatgaac
      661 atgttgctat ccctaactac cttgaaaggc agttcgccgt gaccgagcca aatcaggtgt
      721 ggtgcggtga tgtgacctat atctggacgg gtaagcgctg ggcgtacctc gccgttgttc
      781 tcgacctgtt
//
LOCUS       DAZPDU010000448          789 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZPDU010000448 DAZPDU010000000
VERSION     DAZPDU010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.453"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..458)
                     /locus_tag="ACHURS_004787"
     CDS             complement(<1..458)
                     /locus_tag="ACHURS_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFR1240297.1"
                     /translation="MAHIQLIKQTSSGLLLPATPESCDFLHQIKTGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERELLSGFVDYLCESVGREHTPALSEAAAQ
                     YLNTVATRRTRDTALLKSFEAFREWVTIQAGFYTEHFYPDGSRGRRAKSIA"
     gene            complement(530..742)
                     /locus_tag="ACHURS_004788"
     CDS             complement(530..742)
                     /locus_tag="ACHURS_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240298.1"
                     /translation="MEKIKKLFSSKYAVIRRDDLSVIVEMDYFPETKKSMMYRNGRKA
                     IFLPMRVSDIMGNDKLLDELRVRASC"
BASE COUNT          203 a          193 c          174 g          219 t
ORIGIN      
        1 gcgatggatt ttgcccgacg cccgcggcta ccgtccggat aaaaatgttc ggtgtaaaat
       61 ccagcctgaa tggttaccca ctcgcgaaaa gcctcaaacg actttagcaa tgccgtatcc
      121 cgggttctgc gtgtcgcaac tgtattcaga tattgcgctg cggcttcgct cagagctggc
      181 gtatgttccc gacctactga ttcgcacagg taatcaacga aaccagacag cagttctcgt
      241 tcgcgaggcg tgatcgcccc accgaccgga gtccagtaat cgaatcccag ttgcaggagt
      301 ttgaaaaaac gcttgtggaa tgcgtagtta cgcacacgct taaagtctgc gtgtatccac
      361 tcacctgttt tgatttgatg cagaaaatcg caactctccg gcgtcgccgg gagaagtaat
      421 ccggaagagg tttgtttgat cagttgtata tgcgccatcg tagttctccg ctggcgcagt
      481 agaatgggtg ttcagcccgt tatgtagtat accagaatta atgccaatac taacaggatg
      541 ctctgactcg caattcatcc agcagtttat catttcccat aatgtcactt accctcatcg
      601 gtaaaaaaat tgcctttcga ccattacgat acatcattga ttttttggtt tcagggaagt
      661 aatccatttc gactataact gacaggtcat cacgacgtat gactgcgtat ttactactaa
      721 atagtttctt tattttttcc acgatgcctc caggtttata agtacaaacg gttatatcaa
      781 catagagac
//
LOCUS       DAZPDU010000449          786 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZPDU010000449 DAZPDU010000000
VERSION     DAZPDU010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..786
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.454"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..623
                     /gene="yahL"
                     /locus_tag="ACHURS_004789"
     CDS             <1..623
                     /gene="yahL"
                     /locus_tag="ACHURS_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HFR1240299.1"
                     /translation="KLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMDYPDHPVK
                     FVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFINSVRTDCKCILLEH
                     HMPLLRLFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYPVFPESLV
                     MLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
BASE COUNT          229 a          157 c          168 g          232 t
ORIGIN      
        1 acaaactcat tcatctcatg gctgccgtac gagactgtga gtggtcagac gataacgcac
       61 tcaccataaa tgtgcagttt aatgatttcc caggatttta tgactggatg gattaccctg
      121 atcacccggt taagtttgtt tttcgcatac ttgaaaatca aaaagggaca gtttgggtat
      181 atgaccagga tgatgcgttt cttgatatta aagcaaatgt gcaggctgga cgctttaccg
      241 ggcttaagaa attagtgcag tttatcaatt cagtgcgaac agattgcaaa tgtattcttc
      301 ttgaacacca tatgcctttg ctgagacttt tccctaaagg gaaagagtgt atgcatgtgg
      361 aaaaatggct tcgcgaaatg tcgtctattc cggagacaga cgcgcccatt aagcaggcgc
      421 ttgcgcatgg attattatta cacttaaaaa atatctaccc tgtttttcct gaatcactgg
      481 tgatgttatt gctctctgtt ctggacgtaa aaacatacag agatgatgca cgattaaatg
      541 aatggatatc taatagagtg caggaattag gtgacagata ttatcctgta aataagcacg
      601 ttaaaatccg ctacactctt tagttcagaa agggttccac ctgcaagttg ttattccacc
      661 agagagtgaa tcgcaaagca ggtaatcatt tcatctgtat atccgtatgc caaatggcat
      721 tccaccgcct tttcgccgat attttgttgt gatcggcgac acttcgctta aaaaagcacc
      781 agcagt
//
LOCUS       DAZPDU010000450          775 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZPDU010000450 DAZPDU010000000
VERSION     DAZPDU010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.455"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            40..756
                     /locus_tag="ACHURS_004790"
     CDS             40..756
                     /locus_tag="ACHURS_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR1240300.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNAGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          257 a          135 c          166 g          217 t
ORIGIN      
        1 taccagtaat gtaaataaat tttcgaggag atcattccag tgggacgtaa atgggccaat
       61 attgttgcta aaaaaacggc taaagacggt gcaacgtcta aaatttatgc aaaattcggt
      121 gtagaaatct atgctgctgc taaacaaggt gaacccgatc cagaattaaa cacatcttta
      181 aaattcgtta ttgaacgtgc aaagcaggca caagttccaa agcacgttat tgataaagca
      241 attgataaag ccaaaggcgg cggagatgaa acgttcgtgc agggacgtta tgaaggcttt
      301 ggtcctaatg gctcaatgat tatcgccgag acattgactt caaatgttaa ccgtacgatt
      361 gctaacgttc gcacaatttt caataaaaaa ggcggcaata tcggagcggc aggttctgtc
      421 agctatatgt ttgacaatgc tggtgtgatt gtatttaaag gatcagaccc tgaccatatt
      481 tttgaaattt tacttgaagc tgaagttgat gttcgtgatg tgactgaaga agaaggtaac
      541 attgttattt ataccgaacc tactgacctt cataaaggaa tcgcggctct aaaagcagct
      601 ggaatcactg agttctcaac aacagaatta gaaatgattg ctcaatctga agttgagctt
      661 tccccagaag atttagaaat ctttgaaggg cttgttgatg cccttgaaga tgacgacgat
      721 gtacaaaaag tttatcataa cgtcgcaaat ctctaattat cttttaaaga aatct
//
LOCUS       DAZPDU010000451          772 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZPDU010000451 DAZPDU010000000
VERSION     DAZPDU010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.456"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..431)
                     /locus_tag="ACHURS_004791"
     CDS             complement(<1..431)
                     /locus_tag="ACHURS_004791"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR1240301.1"
                     /translation="MVKSMKTNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIR"
     gene            488..757
                     /locus_tag="ACHURS_004792"
     CDS             488..757
                     /locus_tag="ACHURS_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR1240302.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQEFSEYIAPLQD
                     AVDLEIATEEERSLLEAWNKYRVLLNRVDTSTAPDIEWPANPVRE"
BASE COUNT          227 a          146 c          177 g          222 t
ORIGIN      
        1 ttgcgtatta cacagctttt tgtagatata tttccaggca ggcagtgaag aaggtaaaac
       61 atggaaatgg aatcaaattg accatgtaac gccgcgggat aaggttcaaa aacatcatgg
      121 ctaattttat gtttaatttt tgattcccca gcccttgtag atgccgcgtt caggctagct
      181 tcgttcaaat ccattaaaga tatcagacta ctctcaggta cgtgagtaag gtaaaaccca
      241 gttccaacac caatatccag atggttgtta cctaaatgtt ccagaaagtg tggaagaagg
      301 tgttcctttg taggacatcc ccatgcaagc cgatttgata ctcccaaaac ccaccagtca
      361 taaagcttta gggtaagtgg tgtgtaaatt ttagccccat catctgtgtt tgttttcatt
      421 gatttcacca tgttatagtt ttatttgtga attaaatcaa ttatggcgat gaattacaag
      481 gggttaaatg ctgccgcagc atagcgatat tgaaatagcc tggtatgctt cgatacagca
      541 ggagccgaat ggctggaaga ccgtcaccac acagttctac atccaggaat ttagtgagta
      601 tattgcgcca ctgcaggatg ctgtagatct ggaaatcgca acggaggaag aaagatcgtt
      661 gctggaggca tggaataaat atcgggtatt gttgaatcgt gttgatacat caactgcacc
      721 tgatattgag tggcctgcaa atcctgtcag ggagtaatca ttgggattat gc
//
LOCUS       DAZPDU010000452          769 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZPDU010000452 DAZPDU010000000
VERSION     DAZPDU010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.457"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..543
                     /locus_tag="ACHURS_004793"
     CDS             <1..543
                     /locus_tag="ACHURS_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240303.1"
                     /translation="KPASGRAVLSQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKS
                     TNNTGAHTHSLSGSTSSAGAHQHSQTGPRTNSGSQPTGMFPAGSTQVSGTNQVGISGS
                     LTSGTSQWVGKSSSEGNHTHSLSGTAASAGAHAHTVGIGAHTHSVAIGSHGHTITVNA
                     AGNAENTVKNIAFNYIVRLA"
     gene            543..769
                     /locus_tag="ACHURS_004794"
                     /pseudo
     CDS             543..769
                     /locus_tag="ACHURS_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
BASE COUNT          211 a          195 c          190 g          173 t
ORIGIN      
        1 aagcccgcca gtggtcgtgc cgtattgtct caggaacagg atggcattaa atcgcacacc
       61 cacagcgcca gcgcatccag tacggatttg gggacgaaaa ccacatcgtc gtttgattac
      121 ggcactaaat ccacgaataa caccggggcg catacgcaca gtctgagcgg ctctacaagt
      181 tcagctgggg cccaccaaca tagtcagact gggcccagaa cgaatagcgg gagtcaacct
      241 actggaatgt ttccggcagg gagtactcaa gttagcggga ctaatcaggt tggtatcagt
      301 ggctcattaa ccagtggcac ctcccaatgg gtaggtaaat catcttcaga aggaaaccat
      361 acacactcac tgtccggcac agcagcatct gcgggtgcac acgcgcatac tgtcggtatt
      421 ggtgctcata cgcactctgt tgcgattggt tcacatggac acaccatcac cgttaacgct
      481 gctggtaacg cggaaaacac cgtcaaaaac atcgcattta actatattgt gaggctcgca
      541 taatggcatt cagaatgagt gaacaagcac ggaccataaa aatttataac ctgctggccg
      601 gaactaatga atttattggt gaaggtgacg catatattcc gcctcataca ggtctgcctg
      661 caaacatcgt ggcatctcgt tgaagatcat cggggtaaaa cggtttatga cgtggcttcc
      721 ggcgacgcgt tatttatttc tgaactcggc tcattaccgg aaaatgtca
//
LOCUS       DAZPDU010000453          766 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZPDU010000453 DAZPDU010000000
VERSION     DAZPDU010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..766
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.458"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..>766
                     /locus_tag="ACHURS_004795"
     CDS             <1..>766
                     /locus_tag="ACHURS_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1240304.1"
                     /translation="YNRSNVVMPDDGAPFRYSFSALKDRHNAVEVNWIDPDNGWETAT
                     ELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLWLIKTELLETQTVDFSVGAEGLR
                     HVPGDVIEICDDDYAGIRTGGRVLAVNSQTRTLTLDREITLPSSGTTLISLVDGQGNP
                     VSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTLHQRLFRCVSIRENDDGTYAITA
                     VQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHLT"
BASE COUNT          160 a          206 c          259 g          141 t
ORIGIN      
        1 cctataaccg cagtaatgtg gtgatgccgg atgatggcgc gccgttccgc tacagtttca
       61 gcgccctgaa agaccgccat aatgccgttg aggtgaactg gattgacccg gataacggct
      121 gggagacggc gacagagctt gtggaggata cgcaggccat tgtccgttac ggtcgtaacg
      181 tcacgaagat ggatgccttt ggctgtacca gccgggggca ggcacaccgc gccgggctgt
      241 ggctgattaa aacggaactg ctggagacgc agaccgtgga tttcagcgtg ggtgctgaag
      301 ggcttcgtca tgtaccgggc gatgtcattg aaatctgcga tgatgactat gccggtatcc
      361 gcaccggcgg gcgcgtgctg gcggtgaaca gccagacccg gacgctgacg ctcgaccgtg
      421 aaatcacgct gccatcctcc ggcaccacgc tgataagcct ggttgacggg caggggaatc
      481 cggtcagcgt ggaggtccag tccgtcaccg acggcgtgaa ggtaaaagtg agccgtgttc
      541 ctgacggtgt tgctgaatac agcgtgtggg ggctgaagct gccgacgctg caccagcgat
      601 tgttccgctg cgtgagtatc cgtgagaacg acgacggcac gtatgccatc accgccgtgc
      661 agcatgtgcc ggaaaaagaa gccatcgtgg ataacggggc gcactttgac ggcgaccaga
      721 gcggcacggt gaatggtgtc acgccgccag cggtgcagca cctgac
//
LOCUS       DAZPDU010000454          761 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZPDU010000454 DAZPDU010000000
VERSION     DAZPDU010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.459"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..516
                     /locus_tag="ACHURS_004796"
     CDS             <1..516
                     /locus_tag="ACHURS_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSso6 family transposase"
                     /protein_id="HFR1240305.1"
                     /translation="LESALKEAVTEWRLYPVVEALQAMRGIQFITAVGLISELGDLTR
                     FEHPRQLMSWFGITPSEYSSGGSRHQGSITKAGNSYARKLLVEAAWSYRHPARISPAI
                     QKRQENLPRPVIDRAWDAQLRLCKRYRKLQAKGKNVNITIVAVARELAGFIWDMGRIA
                     MSVAQQPQCHK"
BASE COUNT          222 a          174 c          198 g          167 t
ORIGIN      
        1 ctggaatccg ccctgaagga ggccgttacc gaatggaggc tgtatccggt agtagaggcg
       61 ctgcaagcca tgcgagggat tcagtttatt actgcggttg gattaatttc tgaactgggt
      121 gatctgaccc gttttgaaca tccacgacaa ctgatgtcat ggtttggtat cacgccatca
      181 gaatattcat cgggtggaag cagacatcag ggcagcatta ccaaagccgg aaacagctat
      241 gccagaaagc tattggtcga ggcagcgtgg agttaccgtc atccggcacg catcagtccg
      301 gcaatacaaa aaaggcagga aaatttaccc cgccccgtca ttgacagagc atgggatgct
      361 caactcaggc tttgtaagag gtatcgaaaa cttcaggcca aaggaaagaa tgtcaatatt
      421 acaattgttg ctgttgcacg tgagctggcg ggttttatct gggatatggg ccgaatagca
      481 atgtctgtcg cacaacaacc acaatgtcac aaataaaaaa tgagaataac cagttccctg
      541 aaggcgacgt agcccggtac aagagtaacc cacgttagcg cttggcgacg gcggaacaaa
      601 gctgattcgc gacgtaagaa agtggcaggc tcatatgacg gacctctgta atgcggtatc
      661 caacccgcga atatcagcgt gattcaccgt cggatttact gctacgtcgc cctcagggaa
      721 ttaaacctaa aaagaaaatc gtcaaaatta gagattccgg t
//
LOCUS       DAZPDU010000455          751 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZPDU010000455 DAZPDU010000000
VERSION     DAZPDU010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.460"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            584..>751
                     /locus_tag="ACHURS_004797"
     CDS             584..>751
                     /locus_tag="ACHURS_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFR1240306.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVID"
BASE COUNT          213 a          135 c          164 g          239 t
ORIGIN      
        1 cttgcttgca ccgtattgtt cttgtttttc ggttagttaa acatcgtact tcatatttga
       61 acgttctgcc ggaatgcatt atcaatagag gtaaagtcgc aaccccaaat cgtaaaggaa
      121 accgtagcac gtcgtatgca agaacgtgcc acggctggct gatggatgtt cgatagcgcg
      181 agtttgaatg aaaatcagcc ggagatgatt ttacataatt gctacggaat tattcaatac
      241 aggaattgct tgcgtctgca tggattgacc tgaaatattc ccgaaaattt ctctaaaaaa
      301 ctcgaaaaaa atggtaactg gttgaatgta ttaatatgca atggtacgtg tcagggatta
      361 aaagatgaac gtaaatttat ttaacgcatt aattttaaag ggttttattg tttgttgacg
      421 aaaacaggaa tcgtgttcgg tctcttttta tctgttaaaa gccagaagca tttccttcgc
      481 tgactttata gtcaaccata acacacactc tactgtctga gtccaacgtt ttttaacatt
      541 cttgttaaaa ttatgtgatc tttagcgcgg gaggaaaata ttgatgaaac agcctgcgcc
      601 cgtttatcag agaattgcgg gtcatcaatg gcgacatatc tggctttctg gcgatataca
      661 cggttgtctt gagcagttgc gccgcaaatt atggcattgt cgttttgatc cgtggcgaga
      721 tttacttatc tcagtgggag acgttatcga t
//
LOCUS       DAZPDU010000456          748 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZPDU010000456 DAZPDU010000000
VERSION     DAZPDU010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.461"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..407
                     /locus_tag="ACHURS_004798"
     CDS             <1..407
                     /locus_tag="ACHURS_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR1240307.1"
                     /translation="WRVSSSMETTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYT
                     QRLKEAGLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWKNRAEVELAILTWVDWY
                     NNRRLLERLGHTPPAEAEKAYYASIGNDDLAA"
     gene            449..649
                     /locus_tag="ACHURS_004799"
                     /pseudo
     CDS             449..649
                     /locus_tag="ACHURS_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611683.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
BASE COUNT          216 a          175 c          198 g          159 t
ORIGIN      
        1 ggtggcgggt ctcatcgtcc atggaaacga cattcgtgct ggatgcactg gagcaggcgt
       61 tatgggcccg tcgaccgtcc ggcacggtcc atcacagtga taaaggttct cagtatgtat
      121 cgctggccta cacacagcgg cttaaggaag ccggattact ggcatcaaca ggaagtacag
      181 gcgactcgta tgacaacgcg atggcggaga gcatcaatgg tctttacaaa gcggaggtaa
      241 tacaccgtaa gagctggaaa aaccgtgcag aagtggaact ggccatactc acgtgggtgg
      301 actggtataa caatcgacga ttgctggaaa ggctgggcca tactcctccg gcagaagcag
      361 aaaaagctta ttatgcttcc atcggaaacg atgatctggc agcctgagtt cacagataaa
      421 acactctcca ggaaacccgg ggcggttcaa ccttcacgat aaaaaacttt tcattaaagg
      481 taaagcggat caatatccgg cacttcattc tctgatatct ccgacccgca ttaatctgaa
      541 agaaatagaa attcactggc gtgaagtgct acgtctggcg acctcaataa aacagggaac
      601 agtgacagca tccctgatgc tgagaaaact tgccagttac cccaaacaaa agtaaggcag
      661 tatgactttc cgtgagacag atgctggcca acgtgggata tttaacgcgg gttaacatcg
      721 ttgtcagagc cgcatgggtg agctgttg
//
LOCUS       DAZPDU010000457          746 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZPDU010000457 DAZPDU010000000
VERSION     DAZPDU010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.462"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..400
                     /locus_tag="ACHURS_004800"
     CDS             <1..400
                     /locus_tag="ACHURS_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HFR1240308.1"
                     /translation="DVINAIVKPFYERQMSKSDNPIDKVVRSKVEPTDQALTGDMNSL
                     AAVLWAMVQGKSKDVPQFSPEALNNLKGRVETFTQDLKVIKPSVNIQPQTINITTQTV
                     LDGKVIDERTTSHINRMQEDTLISSAYPEE"
BASE COUNT          261 a          138 c          141 g          206 t
ORIGIN      
        1 tgatgtgatt aatgccattg tcaaaccgtt ctatgaacgt cagatgagta agtcagataa
       61 tccaattgat aaagttgtaa ggtcaaaggt ggaacccaca gaccaagcat tgactggtga
      121 catgaactct ttagccgctg ttttatgggc tatggttcag ggcaaatcaa aagatgttcc
      181 acagttttca cctgaagcat taaataatct gaaaggtcga gtggaaacat tcacacaaga
      241 tttaaaggtt atcaaaccgt cagtaaatat tcagcctcag acaatcaata tcacaactca
      301 gacggtactt gatggaaagg ttattgatga aagaaccaca tcgcatatta atcgtatgca
      361 ggaagatacc ttgattagct cagcttatcc agaggaataa acaatgttag agctactaat
      421 atttattgcc acacatggca atctattaat ctttatctag tatgacgatt caactatgaa
      481 tagtcgaatg ctacgtgaaa tcttctgaac agataccaac atcaaaaatt agatttcacg
      541 tagctcacgc ttaacagaat aggaataaca acaatgtttg acacaaagca aattagatac
      601 tcaggtaaag atggtatcta ttttcacctt agagataatg tggaatcttt tcttgcctta
      661 acagctactg aacagatgga ctatgactca ccgatgatgg tgacaactca ggctatgcaa
      721 tcggggcaaa ctatcactga taatgt
//
LOCUS       DAZPDU010000458          736 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZPDU010000458 DAZPDU010000000
VERSION     DAZPDU010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.463"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(1..736)
                     /locus_tag="ACHURS_004801"
                     /pseudo
     CDS             complement(1..736)
                     /locus_tag="ACHURS_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
BASE COUNT          195 a          195 c          158 g          188 t
ORIGIN      
        1 caaaaaggca aacagatccg aagcgggata atctttttgg ggagaattgt tccagttagc
       61 aatatatccc gacagggggt tatacacctt agggttcatt tcaaaaggca atagcccttt
      121 ccagtcccat tttcccgtac caggaacggg taatcgcgga tcatggcctg attgacgatc
      181 tggataagca ccagtatgaa cataaccaat attgccgttt acatcagcat agtaccagtt
      241 gatggtcagt gcttgtttcg ctgcctgctg tgtccactcc tgccaatttt tggccttcat
      301 ctgatgagtc caggccagca aagacgccac ctctttacca tcccatgcgc gggatttagc
      361 gtaagccgtt tgtgtcgtct ggtcagtttg gagaatgttg ccatgcaccg tacgccagac
      421 agtaaaggtc tctgcctgac catttttcac cgtaatggtt tcctcacggc ttaacatttt
      481 cacccactta ccattatgca agtagtagcc tggtttctct gccgacagcc gttcagcaaa
      541 aatatcgaca tcatcgccga aacctgccgt tgatccccag gaaatcacac cattatgacc
      601 aaaaaccagc ccaggatagg ataggcaaat ggtgtattgc cagtgacatc ataaccagca
      661 ccgtgcagac caataccata agtatacgca ggcgcatacc agccaaactg cggaccattt
      721 accatgattg ctttcg
//
LOCUS       DAZPDU010000459          727 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZPDU010000459 DAZPDU010000000
VERSION     DAZPDU010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..727
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.465"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..>727
                     /locus_tag="ACHURS_004802"
     CDS             <1..>727
                     /locus_tag="ACHURS_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240309.1"
                     /translation="INKLESLPKIAQQKAKTEKQNQRAQAAAARAQARQQQQIAARKE
                     KAELKLLDVSSSISAMHRLSVAEQYKAIAQAREIAKQYEQGAISLARMNSQMKRLQQQ
                     QRKINGNRKAQFAPVKGGSGNSATMGALLFGGATAAAGVMAVSKTSEFMANSFANAET
                     EGELIQRARLGGVDYNLLNNLERWAYDNGIDSLQGDPGRRKILDAIGKDVKERATKSY
                     DEAEYVVDKKTGKGIWKGGDSGI"
BASE COUNT          248 a          144 c          187 g          148 t
ORIGIN      
        1 ctatcaataa gttggagtca ttacccaaga ttgctcaaca gaaagctaaa acagaaaagc
       61 agaatcaaag agcacaagct gccgcagcaa gagcacaggc aaggcaacag caacaaatag
      121 cagcacgtaa agagaaagca gaattaaaac tccttgatgt atcaagctct atcagtgcaa
      181 tgcaccgtct ctctgtagcg gaacaataca aagctattgc acaggctaga gagatagcaa
      241 aacaatatga gcaaggggct atcagcctgg cgcgtatgaa tagtcagatg aaacgcctac
      301 aacagcaaca acgtaagatt aatggcaaca gaaaagctca atttgccccc gttaagggag
      361 gatcagggaa ttccgccacg atgggcgctc tgttatttgg aggcgctacg gctgctgctg
      421 gcgtgatggc tgttagtaaa acctctgaat ttatggctaa cagctttgct aatgctgaaa
      481 cagaagggga gctaatccaa cgtgcaaggc ttggtggagt tgattacaac ttacttaata
      541 atcttgagcg ttgggcgtat gacaacggga tcgatagcct ccagggtgat ccgggacgca
      601 gaaaaatact agatgccatc ggtaaagatg taaaggaacg cgctaccaaa agctatgacg
      661 aggctgaata tgtagttgat aagaaaacag ggaaaggcat atggaagggc ggtgattctg
      721 gtattaa
//
LOCUS       DAZPDU010000460          726 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZPDU010000460 DAZPDU010000000
VERSION     DAZPDU010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.466"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            35..>726
                     /locus_tag="ACHURS_004803"
     CDS             join(35..284,284..>726)
                     /locus_tag="ACHURS_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743396.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
                     /protein_id="HFR1240310.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YTASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTILRHFKKLRPQSVTSRIQPGSDV
                     IVCAEMDEQWGYVGAKSRQRWLFYAYDSLRKTVVAHVFGERTMATLGRLMSLLSPFDV
                     VIWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELH
                     DKVIGHYLNIKHY"
BASE COUNT          167 a          183 c          205 g          171 t
ORIGIN      
        1 atttagtgta tgatggtgtt tttgaggtgc tccagtggct tctgtttcta tcagctgtcc
       61 ctcctgttca gctactgacg gggtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcactgcc gtaaaacatg gcaactgcag ttcacttaca ccgcttctca
      181 acccggtacg caccagaaaa tcattgatat ggccatgaat ggcgttggat gccgggcaac
      241 tgcacgcatt atgggcgttg gcctcaacac gattttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acagccgggc agtgacgtca tcgtctgcgc ggaaatggac
      361 gaacagtggg gctatgtcgg ggctaaatcg cgccagcgct ggctgtttta cgcgtatgac
      421 agtctccgga agacggttgt tgcgcacgta ttcggtgaac gcactatggc gacgctgggg
      481 cgtcttatga gcctgctgtc accctttgac gtggtgatat ggatgacgga tggctggccg
      541 ctgtatgaat cccgcctgaa gggaaagctg cacgtaatca gcaagcgata tacgcagcga
      601 attgagcggc ataacctgaa tctgaggcag cacctggcac ggctgggacg gaagtcgctg
      661 tcgttctcaa aatcggtgga gctgcatgac aaagtcatcg ggcattatct gaacataaaa
      721 cactat
//
LOCUS       DAZPDU010000461          715 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZPDU010000461 DAZPDU010000000
VERSION     DAZPDU010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..715
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.467"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..225
                     /locus_tag="ACHURS_004804"
     CDS             <1..225
                     /locus_tag="ACHURS_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HFR1240311.1"
                     /translation="RSHWSTSKTPSYTKSVSWQHHPLTIPCKSDPDLCMQLDAWDAET
                     SIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            330..521
                     /locus_tag="ACHURS_004805"
     CDS             330..521
                     /locus_tag="ACHURS_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFR1240312.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(522..>715)
                     /locus_tag="ACHURS_004806"
     CDS             complement(522..>715)
                     /locus_tag="ACHURS_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HFR1240313.1"
                     /translation="VDLHQVLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNL
                     HYIDTGAVFGGELTLVQLQ"
BASE COUNT          183 a          180 c          158 g          194 t
ORIGIN      
        1 agaagccact ggagcacctc aaaaacacca tcatacacta aatcagtaag ttggcagcat
       61 cacccgttaa ccattccttg taaatctgac cccgatttat gtatgcaact cgatgcctgg
      121 gatgctgaaa ccagtatccc tgccctgcta aatggcgaac actctgtcct ttaccgtacc
      181 cgttacgatc aacaatctga tgcctggatt atgcgtcttg cctgatccaa aaagaacccg
      241 tcgggatggc gggttatttg tcctggttat tcccccgttg taaaatctct cctaaactta
      301 acggtacggc accacacttc ggggatgaaa tgttcgcgct ggtacttttt gtttgctacc
      361 tggatggcgg ttgtgaagat attgttgtgg atgtctacaa cacggaacag cagtgtcttt
      421 attctatgag cgatcaacgg atccgccatg gcggttgttt tccgattgag gattttatag
      481 atggtttctg gcgacctgca caggagtacg gtgattttta attattgcaa ttgcacaaga
      541 gtcagttcgc ccccaaagac agcaccggta tcaatataat gcaggttgcc aatatccacg
      601 cgatgtcgca acggtgtatg accaaaccag aaatgatcag cacctgtaat tccctgccct
      661 ttttggcgtt cacctaatcg cgagcggctc cacaagacct gatgcaaatc aacgt
//
LOCUS       DAZPDU010000462          710 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZPDU010000462 DAZPDU010000000
VERSION     DAZPDU010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.468"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            31..>710
                     /locus_tag="ACHURS_004807"
     CDS             31..>710
                     /locus_tag="ACHURS_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR1240314.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTL"
BASE COUNT          223 a          151 c          127 g          209 t
ORIGIN      
        1 tttatcagaa tatccttctg cgaggttatt atgcttcctg taaataatcc ccccctatcc
       61 actggaaacg tctcttttta cagaactaca tcaatcgaca atgttcacaa taattatctc
      121 tccgaatggg ttgaatggac taaaaacagc atttccggag aaaacaggga aactgctttt
      181 acccggctcc aattatgtct ggagaacagt gaaacatcgt tggacttatc ttgtttaggt
      241 ctcagatctc taccacgatt gcctgacaat cttgatgaaa ttaatgtaag caataaccaa
      301 ctatcaatgc tccccgagct accaagggca ttgaaagagc tgaatgcaag cagtaatcaa
      361 ttatctgcac ttcctgaatt accagtgtcg ctggaatata taaatgtgag tgataaccat
      421 ttgttcgcac ttcctgaatt acctgcgtca ctagaatata ttaatgtaag tgacaatcac
      481 ctgtctgtac ttccgaggtt accaatgtca ttggaattac ttgatgcagc cagaaatgct
      541 ttggaagtaa taccagattt tccagaaaga gatgatcata ttataagaat attctggctt
      601 aatcagaacc ggatcacggc aattccggaa agcatacttg gcctcagttc tgatagcgtt
      661 gtcaatctta gagaaaatca actatctccc agaataatgc aaactttgtt
//
LOCUS       DAZPDU010000463          695 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZPDU010000463 DAZPDU010000000
VERSION     DAZPDU010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.469"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..>695
                     /locus_tag="ACHURS_004808"
     CDS             <1..>695
                     /locus_tag="ACHURS_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR1240315.1"
                     /translation="VDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLG
                     RQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKALEG
                     DAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKV
                     GEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPG
                     TIKPHTKFESEVY"
BASE COUNT          162 a          167 c          205 g          161 t
ORIGIN      
        1 gtagactgcc cggggcacgc cgactatgtt aaaaacatga tcaccggtgc tgctcagatg
       61 gacggcgcga tcctggtagt tgctgcgact gacggcccga tgccgcagac tcgtgagcac
      121 atcctgctgg gtcgtcaggt aggcgttccg tacatcatcg tgttcctgaa caaatgcgac
      181 atggttgatg acgaagagct gctggaactg gttgaaatgg aagttcgtga acttctgtct
      241 cagtacgact tcccgggcga cgacactccg atcgttcgtg gttctgctct gaaagcgctg
      301 gaaggcgacg cagagtggga agcgaaaatc ctggaactgg ctggcttcct ggattcttac
      361 attccggaac cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc
      421 tccatctccg gtcgtggtac cgttgttacc ggtcgtgtag aacgcggtat catcaaagtt
      481 ggtgaagaag ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt
      541 gaaatgttcc gcaaactgct ggacgaaggc cgtgctggtg agaacgtagg tgttctgctg
      601 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      661 aagccgcaca ccaagttcga atctgaagtg tacat
//
LOCUS       DAZPDU010000464          690 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZPDU010000464 DAZPDU010000000
VERSION     DAZPDU010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.470"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
BASE COUNT          241 a          105 c          143 g          201 t
ORIGIN      
        1 agtctatttt ataaaaaaat taagtaggct aagaagtacc ttagaaatca attttaaggg
       61 gtctaaaacg ctctgtaacg agctaaactg tataagtggc ataagatgcc ttttagactg
      121 acactcgctt agagagcttc ctagagcttc tgggaggatt cattagatat cgttgtatac
      181 agagacacag tatctataaa gggtattaag agattcttag aagttactgt aaagaaccac
      241 taagataaga agagagggac aaagaaccga aggaaagtcc cttattaagt aggagtctta
      301 atagactgtc ttaatagata acttaaaagt cttaaaggat atcttccaag aagagaggaa
      361 cgaagaacca gaggaagtga ccgttgaccg tttgaacaga ctgtttaagt aaagcctata
      421 aagtatctat taattaaggg ctatgtctca atactagcag tttgtactag cagtttgtca
      481 atacctttgt ctttagcaat tcgtgctatt tattaaatat agctgttaaa acagagtatg
      541 ctttttaacc taaaaccttg tcgcttcgct ctgggtcaca tgttcttggg ttcttagtga
      601 gagttacact atcttctaag gtactcaata aaaagacgag taacaaccga agaacgagga
      661 tagaggtatt gtaataactt agaaggtgtc
//
LOCUS       DAZPDU010000465          684 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZPDU010000465 DAZPDU010000000
VERSION     DAZPDU010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.471"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..683
                     /locus_tag="ACHURS_004809"
     rRNA            <1..683
                     /locus_tag="ACHURS_004809"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          157 a          151 c          230 g          146 t
ORIGIN      
        1 atccgggttg cggacagtgt ctggtgggta gtttgactgg ggcggtctcc tcctaaagag
       61 taacggagga gcacgaaggt tggctaatcc tggtcggaca tcaggaggtt agtgcaatgg
      121 cataagccag cttgactgcg agcgtgacgg cgcgagcagg tgcgaaagca ggtcatagtg
      181 atccggtggt tctgaatgga agggccatcg ctcaacggat aaaaggtact ccggggataa
      241 caggctgata ccgcccaaga gttcatatcg acggcggtgt ttggcacctc gatgtcggct
      301 catcacatcc tggggctgaa gtaggtccca agggtatggc tgttcgccat ttaaagtggt
      361 acgcgagctg ggtttagaac gtcgtgagac agttcggtcc ctatctgccg tgggcgctgg
      421 agaactgagg ggggctgctc ctagtacgag aggaccggag tggacgcatc actggtgttc
      481 gggttgtcat gccaatggca ctgcccggta gctaaatgcg gaagagataa gtgctgaaag
      541 catctaagca cgaaacttgc cccgagatga gttctccctg accctttaag ggtcctgaag
      601 gaacgttgaa gacgacgacg ttgataggcc gggtgtgtaa gcgcagcgat gcgttgagct
      661 aaccggtact aatgaaccgt gagg
//
LOCUS       DAZPDU010000466          684 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZPDU010000466 DAZPDU010000000
VERSION     DAZPDU010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.472"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..560
                     /locus_tag="ACHURS_004810"
     CDS             <1..560
                     /locus_tag="ACHURS_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFR1240316.1"
                     /translation="RFSQLESALNTQKNSIPALEKEVKALDKQMVAAQKAADAYWGKD
                     ANGKQMTREEAFKKIHQQRDEFNKQNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVP
                     IQQKRDFDINEQRRQTFLQSQKLSRKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMK
                     IDDINQANERWKKDTEQLRRNGVIK"
     gene            complement(616..>684)
                     /locus_tag="ACHURS_004811"
     CDS             complement(616..>684)
                     /locus_tag="ACHURS_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240317.1"
                     /translation="ALMMITYIPQISLFIPQLLGLM"
BASE COUNT          244 a          122 c          165 g          153 t
ORIGIN      
        1 atcgctttag tcagcttgaa tctgcgttga atacccagaa gaactcgatt cctgcgctgg
       61 aaaaggaagt gaaagcgctt gataagcaaa tggtggcggc gcagaaagca gcggatgcat
      121 attggggaaa agatgcaaac ggtaagcaaa tgacccgcga agaagccttt aaaaaaattc
      181 accaacagcg tgatgagttt aataaacaga acgatagcga ggcgttcgcg gttaaatatg
      241 acaaagaggt ttatcaaccg gcgattgcgg catgtcataa acagagtgaa gagtgttatg
      301 aagtgccgat tcagcagaaa cgagatttcg atatcaacga acaacggcga cagacttttc
      361 tgcaatcaca aaaactcagc cgtaaattgc aggatgactg ggtaacgctc gaaaaagggc
      421 aatatccgtt aacgatgaaa gtttcagaaa taaacagtaa aaaagtcgcg attttgatga
      481 aaattgacga tatcaaccaa gctaatgaac gctggaaaaa agataccgaa caactgcggc
      541 gcaatggggt gattaagtaa ctcatgttaa gtgcctttgc cggataatat aaaaagttat
      601 ccggcaaatt attttttaca ttagccctag tagctgtgga atgaacaagg atatttgcgg
      661 aatgtaagtg atcatcatca acgc
//
LOCUS       DAZPDU010000467          683 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZPDU010000467 DAZPDU010000000
VERSION     DAZPDU010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.473"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..>683
                     /locus_tag="ACHURS_004812"
     CDS             <1..>683
                     /locus_tag="ACHURS_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="HFR1240318.1"
                     /translation="DTDVLLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRV
                     YRIMRQNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFAL
                     DCCDREALHWAVTTGGFNSETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANET
                     RQFARMLGLEPKNTAVRSPESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEH
                     YNEWHPHSA"
BASE COUNT          168 a          164 c          210 g          141 t
ORIGIN      
        1 tgatacggat gtgcttctcc gtatacacca tgttatcgga gagctgccca cgtatggtta
       61 tcgtcgggta tgggcgctgc ttcgcagaca ggcagaactt gatggtatgc ctgcgatcaa
      121 tgccaaacgt gtttaccgga tcatgcgcca gaatgcgctg ttgcttgagc gaaaacctgc
      181 tgtaccgcca tcgaaacggg cacatacagg cagagtggcc gtgaaagaaa gcaatcagcg
      241 atggtgctct gacgggttcg agttctgctg tgataacgga gagagactgc gtgtcacgtt
      301 cgcgctggac tgctgtgatc gtgaggcact gcactgggcg gtgactaccg gcggcttcaa
      361 cagtgaaaca gtacaggacg tcatgctggg agcggtggaa cgccgcttcg gcaacgatct
      421 tccgtcgtct ccagtggagt ggctgacgga taatggttca tgctaccggg ctaatgaaac
      481 acgccagttc gcccggatgt tgggacttga accgaagaac acggcggtgc ggagtccgga
      541 gagtaacgga atagcagaga gcttcgtgaa aacgataaag cgtgactaca tcagtatcat
      601 gcccaaacca gacgggttaa cggcagcaaa gaaccttgca gaggcgttcg agcattataa
      661 cgaatggcat ccgcatagtg cgc
//
LOCUS       DAZPDU010000468          679 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZPDU010000468 DAZPDU010000000
VERSION     DAZPDU010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.474"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..>679
                     /locus_tag="ACHURS_004813"
     CDS             <1..>679
                     /locus_tag="ACHURS_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR1240319.1"
                     /translation="QMQSQMDPVYTPARKIHLYHCDHRGLPLALISTEGTTAWCAEYD
                     EWGNLLSDENPHHLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGW
                     NFYQYPLNPVQYIDSMGLASKYGHLNNGGYGAKPSKPPTPDPSKLPDIAKQLRLPYPI
                     DQASSAPNVFKTFFRALSPYDYTLYCRKWVKPNLTCMPQDDPQYPGMETKTASDYLPQ
                     TNWSTTQL"
BASE COUNT          195 a          170 c          171 g          143 t
ORIGIN      
        1 gcagatgcaa agccagatgg acccggtata cacgccggcg cgaaaaattc acctgtacca
       61 ctgcgaccat cgcggcctgc cgctggccct tatcagcacg gaagggacaa cagcgtggtg
      121 cgcagaatat gatgaatggg gcaacctgct gagtgatgag aacccgcatc atctgcagca
      181 gctcattcgc ctgccggggc agcagtatga tgaggagtcc ggcctgtatt acaaccgcca
      241 ccgctattat gacccgctgc aggggcggta tatcactcag gatccgattg gactgaaggg
      301 gggatggaat ttttatcagt atccgttgaa tccggttcag tatatagatt caatggggct
      361 ggcatctaaa tatggacact taaataatgg cggatatgga gcgaaaccca gcaaaccgcc
      421 tacgcccgat ccaagtaagt tgccagacat agcgaaacaa ttaagactgc catatcctat
      481 tgaccaggcc agtagtgcgc ctaatgtttt caaaacattc ttcagagcat taagccctta
      541 cgattacaca ctgtattgca ggaagtgggt aaaacccaat ctgacttgta tgccgcagga
      601 tgatccccag tatccaggga tggagacaaa gacagcaagt gattacctgc cacagacaaa
      661 ttggtcaaca actcaatta
//
LOCUS       DAZPDU010000469          669 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZPDU010000469 DAZPDU010000000
VERSION     DAZPDU010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.475"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..>669
                     /locus_tag="ACHURS_004814"
     CDS             <1..>669
                     /locus_tag="ACHURS_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFR1240320.1"
                     /translation="DENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHI
                     DAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV
                     FNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIA
                     KLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEAT
                     DIVVM"
BASE COUNT          133 a          194 c          192 g          150 t
ORIGIN      
        1 gacgaaaaca ccatcggcgt ggtgccgact ttcggcgtga cctacaccgg taactatgag
       61 ttcccacaac cgctgcacga tgcgctggat aaattccagg ccgacaccgg tatcgacatc
      121 gacatgcaca tcgacgctgc cagcggtggc ttcctggcac cgttcgtcgc cccggatatc
      181 gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg cttcaggcca taaattcggt
      241 ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg aagaagcgct gccgcaggaa
      301 ctggtgttca acgttgacta cctgggtggt caaattggta cttttgccat caacttctcc
      361 cgcccggcgg gtcaggtaat tgcacagtac tatgaattcc tgcgcctcgg tcgtgaaggc
      421 tataccaaag tacagaacgc ctcttaccag gttgccgctt atctggcgga tgaaatcgcc
      481 aaactggggc cgtatgagtt catctgtacg ggtcgcccgg acgaaggcat cccggcggtt
      541 tgcttcaaac tgaaagatgg tgaagatccg ggatacaccc tgtacgacct ctctgaacgt
      601 ctgcgtctgc gcggctggca ggttccggcc ttcactctcg gcggtgaagc caccgacatc
      661 gtggtgatg
//
LOCUS       DAZPDU010000470          662 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZPDU010000470 DAZPDU010000000
VERSION     DAZPDU010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.476"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..>662
                     /locus_tag="ACHURS_004815"
     CDS             <1..>662
                     /locus_tag="ACHURS_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR1240321.1"
                     /translation="TFIADDNNAELIPCLSTDLLVSLGIKKIALLDNKEHSAEKHVPD
                     NSACTPLQDRLADASTEFDVGQQHLSLSVPQIYVGRMARGYVSPDLWEEGINAGLLNY
                     SFNGNSINNRSNHNAGKSNYAYLNLQSGINIGSWRLRDNSTWSYNSGSSNSSDSNKWQ
                     HINTSAERDIIPLRSRLTVGDSYTDGDIFDSVNFRGLKINSTEAMLPDRQHGFAPVIH
                     GI"
BASE COUNT          201 a          128 c          145 g          188 t
ORIGIN      
        1 tacttttatt gcagatgata ataacgcaga actcatccca tgcctgagta cagatcttct
       61 tgtcagtctt ggtataaaaa aaatagcgtt attagacaat aaagaacatt cggcagaaaa
      121 acatgtgccg gacaacagcg catgcacacc acttcaggat cgtttggccg acgcatcaac
      181 tgagtttgat gttggtcagc aacatctgtc actctccgtt ccgcaaattt atgttggcag
      241 aatggctcgc ggctatgttt ctcctgatct gtgggaagaa ggaataaatg cagggcttct
      301 aaactatagt tttaatggta attctattaa taatcgtagt aaccataatg caggaaaatc
      361 aaactatgca tatttgaatt tacagagtgg catcaacatt ggtagttggc gactacgcga
      421 taactcaacg tggagttata acagtgggag tagcaattca tctgacagca ataaatggca
      481 gcatatcaat acgtcggctg aacgtgacat tattccctta cgctcacggt taacggtagg
      541 tgatagttat accgatggcg atatttttga tagtgtgaac tttcgtggcc tcaaaataaa
      601 ttcaacagaa gcgatgttgc ccgatagaca acatggtttc gctccggtga ttcatggtat
      661 tg
//
LOCUS       DAZPDU010000471          657 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZPDU010000471 DAZPDU010000000
VERSION     DAZPDU010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.477"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            111..>657
                     /locus_tag="ACHURS_004816"
     CDS             111..>657
                     /locus_tag="ACHURS_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR1240322.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAH"
BASE COUNT          244 a          133 c           89 g          191 t
ORIGIN      
        1 atatttctga tagttataaa ctgaaatttc ttaatcttga ttataacaag ctagcatcgc
       61 tggatgtctc ccggcaagaa tctctgattg agttaagtgc ccatcacaat atgatcactg
      121 accttatatt acacaatcat cccaggatga aaacaatcac tttaaacgac aaccatattg
      181 cacatttaaa cgccaaaaac actacaaaac tggaatattt aaacttaagc aataacaatt
      241 tactgccaac caatgacatt gatcaactaa tatcatcaaa acatctttgg catgtattag
      301 ttaacggcat caacaatgat ccacttgcac aaatgcagta ctggactgca gtaagaaata
      361 taattgatga cactaatgaa gtgaccattg atttatcagg acttaattta accactcaac
      421 caccagggct gcaaaacttc acatctatca atcttgataa taaccaactc acacattttg
      481 atgcaaccaa ctacgataga ctcgtaaagc taagtctgaa tagtaatgct cttgagtcaa
      541 taaattttcc tcaaggcaga aatgtaagta ttacacatat atctatgaat aataatgctc
      601 tcagaaatat tgatatagat aggctttcat cagttactta ttttagtgcg gcacata
//
LOCUS       DAZPDU010000472          649 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZPDU010000472 DAZPDU010000000
VERSION     DAZPDU010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.478"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(26..330)
                     /locus_tag="ACHURS_004817"
                     /pseudo
     CDS             complement(26..330)
                     /locus_tag="ACHURS_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611465.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            384..587
                     /locus_tag="ACHURS_004818"
                     /pseudo
     CDS             384..587
                     /locus_tag="ACHURS_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558123.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          158 a          175 c          153 g          163 t
ORIGIN      
        1 gcatcatttt ttccagaggc ggtcaaactc cgccttcgca aaataagcgg aagcctggcg
       61 aaggatatcg ttactgcggc gcagttcacg attttcacgc tccagctctt tcagacgctg
      121 acgttcagcg gtggtgagcc caccatcacc gcccccggta tcccgctcat gctggcgaac
      181 ccagacacgc agagtctccg gcgtacagcc aatctttggg gcaatggaac aaattgccgc
      241 ccactgtgag tcatattcat cctgactttc cagaaccata cgaatcgccc gctgacggac
      301 ttcgggggaa aaacgagtat ttttagtcat cctgtttacc tctttctcag ggagtttagt
      361 ctccaggatt tccggggcgg ttcagacatc tagaatttac tgtcagcacc ggcgttaaag
      421 tttacttctg cgatcctcag agtccttggc agcggggaac aaatgagaat acaaatgggc
      481 taattcggca gtactttcct aaaaagacat gtcttgccca atatactcaa catcgagtag
      541 gcagcctgac ggctgcggct tgtcatggtc tgcaattacc gttataaaaa caggcaatgt
      601 cattgtcttt caggtggtta tatggcccgt tcagctaaac cccgtaaac
//
LOCUS       DAZPDU010000473          644 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZPDU010000473 DAZPDU010000000
VERSION     DAZPDU010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.479"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..>644)
                     /locus_tag="ACHURS_004819"
     CDS             complement(<1..>644)
                     /locus_tag="ACHURS_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240323.1"
                     /translation="RNGNITRWFYDSSRSGLPCAVEDGTGVRRRITRNRYGQLQAFTD
                     CSGYTTRYEYDRYGQQIAVHREEGISTYSSYNPRGQLVSQRDAQGRETRYEYSAAGDL
                     TAIVAPDGSRSEIQYDAWGKAVSTTQGGLTRSMGYDAAGRITVLTNENGSQSTFRYDP
                     VDRLTEQRGFDGRTQRYQYDLTGKLTQSEDEGLITLWHYDASDRITRRTVNGEPA"
BASE COUNT          107 a          211 c          179 g          147 t
ORIGIN      
        1 gccggttcac cgttcaccgt gcgccgggtg atacggtccg acgcatcgta atgccagagg
       61 gtgataagcc cctcatcttc actctgcgtg agttttccgg tcaggtcata ctggtaacgt
      121 tgcgtccggc cgtcaaagcc gcgctgctcc gtcagcctgt cgaccgggtc ataccggaac
      181 gtggactggc tgccgttctc gttggtcagc acagtgatgc gcccggcggc gtcatacccc
      241 atgcttcgcg tcagaccgcc ctgcgtggtg ctgacggcct ttccccacgc atcatactgt
      301 atctcactgc ggctgccgtc cggggcaacg atagcggtga ggtctcctgc ggcgctgtac
      361 tcataacggg tttcacgccc ctgcgcatcc ctctgactga ccagttgacc gcgcgggtta
      421 taactgctgt aagtgctgat gccttcttcc cggtgaacgg cgatttgttg accgtaccgg
      481 tcatactcat accgcgtcgt gtaccccgag cagtccgtaa acgcctgaag ctgaccgtac
      541 cggtttcgcg ttattctccg cctcaccccg gttccgtctt ccaccgcaca gggcaggcca
      601 ctgcgggaag aatcataaaa ccagcgcgta atgttaccgt tgcg
//
LOCUS       DAZPDU010000474          641 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZPDU010000474 DAZPDU010000000
VERSION     DAZPDU010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.480"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..>641
                     /gene="fliD"
                     /locus_tag="ACHURS_004820"
     CDS             <1..>641
                     /gene="fliD"
                     /locus_tag="ACHURS_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFR1240324.1"
                     /translation="VSTEAGAAPGIYTISVTQLAQAQSLRTDSPTIIASTKDALGDES
                     SDTRTIKITQDGRKEPLEIKLNKDQTSLDEISKAINDADSGISASIVKVKDGNYQLVL
                     TASEGLANKMTISVEGDSKLNDLLAYDSKTNTGNMKELVNAQNAQLNVNGIDIERSSN
                     KITDAPQGVTLDLTKKVTDVRVTVTKSNDKATEAIKGWVDSYNSLIDTFNTLT"
BASE COUNT          217 a          146 c          139 g          139 t
ORIGIN      
        1 aagtttccac cgaagcaggt gctgcccccg gtatctatac catcagcgta acccaactgg
       61 cgcaggcgca atctttacga actgattccc cgacaatcat tgcatcaaca aaagatgcac
      121 tgggtgatga gtcatcagat acacgcacaa taaaaattac ccaagacggt cgtaaagagc
      181 cgctggaaat caagctcaat aaagatcaga cgtcattgga tgagatcagc aaagccatta
      241 acgatgccga tagtggaatt tccgctagta tcgttaaagt caaagatggt aactatcaat
      301 tggtattgac cgccagtgaa ggtctggcca ataaaatgac catatctgtt gagggtgaca
      361 gtaaattaaa tgatctgctg gcttatgaca gcaagaccaa caccgggaat atgaaggaac
      421 tggttaacgc gcaaaatgca cagctaaacg ttaacggtat cgatattgaa cgttcaagta
      481 acaaaattac cgatgctcct cagggagtga ctctcgattt gaccaaaaaa gtcactgacg
      541 tccgggtcac ggtgacaaaa agcaacgata aagcaaccga agcaattaaa ggctgggtgg
      601 attcatataa ctccctcatt gacaccttta acaccctgac a
//
LOCUS       DAZPDU010000475          640 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZPDU010000475 DAZPDU010000000
VERSION     DAZPDU010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.481"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(1..607)
                     /locus_tag="ACHURS_004821"
                     /pseudo
     CDS             complement(1..607)
                     /locus_tag="ACHURS_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
BASE COUNT          190 a          160 c          145 g          145 t
ORIGIN      
        1 tactaccaat ccgattctgt acactatttc cgctgaaatt ataattgagc aagccggcat
       61 taataccggg atcccacaac tcaggaggaa tatatccacg cgcgcgatta ctcataaatg
      121 cctgagggat cgtcaggttg agtcgctgct gaccaacatc taaatgcgca gtagcgtcat
      181 ggatcattga ggttaatggc acgcaggcat catccaccag cagattcata ccggagacag
      241 aagccgtatt cagccccata ctggcaagct gcgcgcgtgt caggcaggga acaatccctt
      301 gttcactgtc gcccgtgtta aatgtgacat cacgcgttgc catataacca ttattcaaat
      361 agatatcgac gcgatacgtc cctggcggta attcttgccc attttcaaaa cgcgataaat
      421 cggccacagc ctagggatca tccgctaaaa agcgcggatt aaaatagagt tcggcagatg
      481 acaaaggtgc ctgtgcggca aaagcacagg cgacaaagag ccggacaaaa agaccagcca
      541 aacgatgctt acgaatatgc aagcattgtg tgtttcgctg gtaaagtctt aaattcagat
      601 atgacattat tcctccggtt taaaatcacc cggcagtcaa
//
LOCUS       DAZPDU010000476          612 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZPDU010000476 DAZPDU010000000
VERSION     DAZPDU010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.482"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            53..>612
                     /locus_tag="ACHURS_004822"
     CDS             53..>612
                     /locus_tag="ACHURS_004822"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HFR1240325.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIR"
BASE COUNT          178 a          142 c          142 g          150 t
ORIGIN      
        1 gctaattctc catctggcgt agggaaaatc acatctgaat caggaattaa caatgaaacc
       61 tgtaaaacca cctcgtatta atggacgagt gccggtcctg tcggcacagg aagcggtgaa
      121 ttatattccc gacgaagcaa cactttgtgt gttaggcgct ggcggcggta ttctggaagc
      181 caccacgtta attacagctc ttgctgataa atataaacag actcaaacgc cacgtaattt
      241 atcgattatt agtccgacag ggcttggcga tcgcgccgac cgtggtatta gtcctctggc
      301 gcaagaaggt ctggtgaaat gggcattatg tggtcactgg ggacaatcgc cgcgtatttc
      361 tgaactcgca gaacaaaata aaattattgc ttataactat ccacaaggtg tacttacaca
      421 aaccttacgc gccgccgcag cccaccagcc tggtattatt agtgatattg gcatcgggac
      481 atttgtcgat ccacgccagc aaggcggcaa actgaatgaa gtcacgaaag aagacctgat
      541 taaactggtc gagtttgata acaaagaata tctctattac aaagcgattg cgccagatat
      601 cgccttcatt cg
//
LOCUS       DAZPDU010000477          604 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZPDU010000477 DAZPDU010000000
VERSION     DAZPDU010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.483"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(40..510)
                     /locus_tag="ACHURS_004823"
                     /pseudo
     CDS             complement(40..510)
                     /locus_tag="ACHURS_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
BASE COUNT          149 a          179 c          142 g          134 t
ORIGIN      
        1 ttataaccga cacccacgat gaaaccgtca gtgcgccaga gacgtgttgc tgtggcgacc
       61 gtcatcactt ccggtcagca catcgtgcgt tttcccctta ttgtcagtta cgcggatata
      121 atccccggaa aaagtcgaca cacggctgta agccacaccc gccatcgcat acgcgctgaa
      181 ccattcattc acgcgcacag acggccccgc catcacgctg aaccagcggt tacgcacgga
      241 atcttcatgc cagcgggtat cgctgtaacg ggtcagctgg cgattcttgt ctcctgcata
      301 gctgaatgac gtcaccagcc ccagcgtatc cgtaaactca taacggtatt tcacgttaat
      361 cccgttcaga tcatcactgc cgggaacgtt ggtccgggca tgaagatacc ccgcgctcag
      421 cgtggactga tgttcagacg cccatgcagg cgcaccggat acggccaggc aaatggctgc
      481 ggacaaaatg gctgcacaaa ctttacgcat aattacctct cgcttttctg caataaaaaa
      541 ggcgtcattc ctgacgccat ttattggggt tataaatatt tcaacgaata ctgatgccgg
      601 aagc
//
LOCUS       DAZPDU010000478          594 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZPDU010000478 DAZPDU010000000
VERSION     DAZPDU010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.484"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..91
                     /locus_tag="ACHURS_004824"
     CDS             <1..91
                     /locus_tag="ACHURS_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240326.1"
                     /translation="GLENAPQTFIGLLKGKNFGKVVIRVAGDD"
     gene            289..>594
                     /gene="mcbR"
                     /locus_tag="ACHURS_004825"
     CDS             289..>594
                     /gene="mcbR"
                     /locus_tag="ACHURS_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFR1240327.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAVE
                     "
BASE COUNT          173 a          121 c          149 g          151 t
ORIGIN      
        1 cggtttagag aatgcgccac agacgtttat cggcctgctg aagggtaaaa acttcggcaa
       61 agtggtgatc cgcgtcgcgg gtgatgatta aagtttcaac ggcggcgtaa gccgccataa
      121 aaatccaggc aaaaagctta ttttcagctt taattaacta accacgtcat tgttctgctg
      181 ctaataacaa caaaggtggg tggtttatga ttattcactt taatacacca ggtgaactcc
      241 ttctgccgtg caggcagggt tggacagaaa acacgcgaag gaattaaaat gcctggaacg
      301 ggaaaaatga aacatgtcag tttgactctg caggttgaga acgacctgaa acatcagctt
      361 agtattggcg cattaaaacc tggcgcacgc ctgattacta aaaatctggc ggagcaatta
      421 ggtatgagta ttacacctgt gcgtgaagca ttattacgtc tggtttcggt gaatgcgctt
      481 tctgtcgcac ctgcacaagc atttacagtt ccggaagtgg ggaaacgtca attagacgaa
      541 atcaatcgga tccgctacga actggaatta atggcagttg ctctggctgt tgaa
//
LOCUS       DAZPDU010000479          589 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZPDU010000479 DAZPDU010000000
VERSION     DAZPDU010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..589
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.485"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..396)
                     /locus_tag="ACHURS_004826"
     CDS             complement(<1..396)
                     /locus_tag="ACHURS_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR1240328.1"
                     /translation="MLTKRILFSVMLIVSPSVVASEKAHELYDSIYGGKPAPDVINTL
                     HKMAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGGIYYD
                     GEQVRVDYAKAYALFNQAAQHGVNLAWSRL"
BASE COUNT          176 a          148 c           95 g          170 t
ORIGIN      
        1 caacctggac catgctaaat tcacaccgtg ctgcgcggcc tgattaaata gtgcatacgc
       61 tttggcataa tccacccgca cctgctcacc gtcgtagtag atacccccca gcgctaacgg
      121 agcttctctg tcaccttgtt tagccgctaa ctcgaaccat ttaatcgctt cctgaacatc
      181 aggtttggta tcataacgag gctgataata ttcccagcct aacaggctct gcgcgtcgat
      241 atctcccgat tcagccattt tatgcagtgt atttataaca tcgggagcag gtttaccacc
      301 gtatatgctg tcatacaatt catgcgcttt ctcgctggcc accacgcttg gtgaaactat
      361 cagcattaca gaaaataata ttcttttagt taacatgtat acctttatgt cctcactgtc
      421 atatatttcc ataataatta accaccaaat atccatttaa tgaataaata cagtgccata
      481 aaggtccaag taataagcac acatttagcc tgaaacatta tcatccatgt caccaaatcg
      541 tttatttagt gagataaaac tagcatattc gtaagataaa aaatgcaca
//
LOCUS       DAZPDU010000480          563 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZPDU010000480 DAZPDU010000000
VERSION     DAZPDU010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.486"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..>563)
                     /locus_tag="ACHURS_004827"
     CDS             complement(<1..>563)
                     /locus_tag="ACHURS_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HFR1240329.1"
                     /translation="VWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGISGFI
                     GRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVRPSAD
                     LFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIREDLW
                     QDEHLLRLCLRQALQLADVVKLSEEEW"
BASE COUNT          146 a          169 c          128 g          120 t
ORIGIN      
        1 ccattcttct tccgagagct tgacgacatc cgccagttgt agcgcctgcc gcaaacacaa
       61 gcggagcaaa tgctcgtctt gccatagatc ttcacgaata ttaggatcga agctgacaaa
      121 acctccggca tgccggatcg ccgtcatcgc agtaaatgcg ctggtacgcg aaggctcggc
      181 agacaacgca attgaacaga gatgtaacca ttcgccatgt cgccagcagg gcaagtctgt
      241 cgtctctaaa aaaagatcgg cactggggcg gaccataaac gtaaatgaac gttccccttg
      301 atcgttcaga tcgacaagca ccgtggatgt ccggtgccat tcatcttgct tcagatacgt
      361 gatatcgact ccctcagtta gcagcgttct ttgcattaac gccccaaaag gatcatcacc
      421 gacccgacct ataaatccac ttattccgcc taatctggcg attcccaccg caacgttagc
      481 tggcgcgccg ccaggacaag gcagtaggcg cccgtctgat tctggcaaga gatctacgac
      541 cgcatcccct aaaacccata ctt
//
LOCUS       DAZPDU010000481          560 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZPDU010000481 DAZPDU010000000
VERSION     DAZPDU010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.487"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..190)
                     /locus_tag="ACHURS_004828"
     CDS             complement(<1..190)
                     /locus_tag="ACHURS_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0001907 - symbiont-mediated killing of host
                     cell [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFR1240330.1"
                     /translation="MYQMEKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIRED"
BASE COUNT          159 a          144 c          110 g          147 t
ORIGIN      
        1 tgtcttctct tattttgaaa tacagatttg tcagataagt cagaagcccc agaagcagac
       61 ttcccagcac accaatcgca gcccactgtg atggactgac ctgatccaac cactgcaaaa
      121 accagtagcc ggcactgccg gcggaggtgc cgtaggcaat gcctgttgat attttttcca
      181 tctgatacat atcccgcccc ccgacagacc tgtgctatcg gaaagaaaaa aggccatcag
      241 caaaactctg atggcctgaa tcacctttac caatattgta tgaaaaaaca cgcacgacta
      301 attgacaata attttcattt ccattaaaaa agatcgcgta gcattcttaa ttcatgagga
      361 actttacccg ccagcaatct gagtagcgaa agctgttcat ccccaatgtt tttgctggcg
      421 ggtccttttt tctacggtcc tcctccccgg aggggggcat ttcattattt ttctaatcgt
      481 tactggtgag aaggcggaag aaaaagccag ttcttcgccc ctaaataaca gcatgattcc
      541 agtccatgca aaatctgatt
//
LOCUS       DAZPDU010000482          555 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZPDU010000482 DAZPDU010000000
VERSION     DAZPDU010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.488"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            78..555
                     /locus_tag="ACHURS_004829"
                     /pseudo
     CDS             78..555
                     /locus_tag="ACHURS_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
BASE COUNT          136 a          121 c          123 g          175 t
ORIGIN      
        1 agctgataga aacagaagcc actggagcgc ctcaaaaaca ccatcataca ctaaatcagt
       61 aagttggcag catcaccgcc gttgtctaca catttattct ctcggtgctg atttaccggg
      121 aagtaaaatg gcgccattta cccaaactga ttctggagtc ggttgttacc acttcgattg
      181 tattactgtt gatcggtttt tccgtgggca tgtcctgggc aatgactaat gctgatattc
      241 cttatatgat cagcgacgca ttgatgggga tttctgataa cccactgatc atattgctgc
      301 tgattaatat cgtcttatta attgtcggca tctttatgga catgacaccg gctgtactaa
      361 tctttacacc gatcttcttg ccgattgcac aagagctggg aatggaccca gtacatttcg
      421 gtattatgat ggtggccaac ctttgtattg ggctgttaac accgccggtg ggtagtgcgc
      481 tatttgttgg ttgttctatc tcgggtgtga agattcaaca tttaatcaaa ccattgctgc
      541 cattttatgc tagca
//
LOCUS       DAZPDU010000483          553 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZPDU010000483 DAZPDU010000000
VERSION     DAZPDU010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.489"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..>553
                     /gene="feaR"
                     /locus_tag="ACHURS_004830"
     CDS             <1..>553
                     /gene="feaR"
                     /locus_tag="ACHURS_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HFR1240331.1"
                     /translation="WQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDAS
                     RPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMNN
                     PALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEWI
                     AGETGMSVRSLYRMFADKGLVVAQ"
BASE COUNT          140 a          125 c          146 g          142 t
ORIGIN      
        1 tggcaggaag taaaaggcag cgacgatgcc tggttttaca ccgtttttca gcttagtggt
       61 caggcaataa tggagcagga tgagcgtcag gtgcagattg gcgctggcga tattacgtta
      121 ctcgatgcct cacgcccctg ttcgctttac tggcaggagt cttctaaaca gatttcatta
      181 cttttgccac gcactctgct ggaacaatat tttccccatc aaaaacctgt ctgcgcagaa
      241 agactggacg ctgacttacc catggtgcaa ctcagtcatc gcctgttaca ggagagcatg
      301 aataatccgg cactttctga aacagaaagt gaagctgcgc tacaggcgat ggtgtgtctg
      361 ctgcgcccgg tacttcatca gcgggaatct gttcaacctc gccgtgaacg tcagtttcaa
      421 aaagtggtta cgttgataga cgataatatt cgcgaagaga tattacgccc ggagtggata
      481 gccggagaga caggtatgtc agtacgtagt ttgtaccgaa tgtttgccga taaaggtttg
      541 gtagtcgcgc aat
//
LOCUS       DAZPDU010000484          546 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZPDU010000484 DAZPDU010000000
VERSION     DAZPDU010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.490"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            1..546
                     /locus_tag="ACHURS_004831"
                     /pseudo
     CDS             1..546
                     /locus_tag="ACHURS_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="Ulp1 family isopeptidase"
BASE COUNT          194 a          120 c           83 g          149 t
ORIGIN      
        1 ccgacttatt aaaaatgcct gtttaaaatt atataattta gcacataccg caaccaactc
       61 ccaccttaaa ccaactggtc cggataattc agatgttctc ctgttcaaaa agctattctc
      121 cccttcgaaa ttaatgacaa ttatcggtga tgaaattcct cttatatctg aaaaacagtc
      181 gctttcaaag gtgcttttaa atgatgagaa taatgaactg agtgatggta caaacttctg
      241 ggataaaaat cgtcaattaa ccacagatga aatagcttgc tatcttcaga agatcgccgc
      301 taatgcaaaa aatactcaag tcaattatcc tactggtctc tacgtcccct actccaccag
      361 aactcacctg gaagacgctc tcaatgaaaa tattaagagc gatccatcat ggccgaatga
      421 agtccagtta ttccccataa atactggcgg acactggata ttagtttcgc tacagaaaat
      481 agtaaataaa aaaataataa actacaaata aaatgcgtca tattcaactc attgcgtgca
      541 ctaggc
//
LOCUS       DAZPDU010000485          546 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZPDU010000485 DAZPDU010000000
VERSION     DAZPDU010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.491"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            1..546
                     /locus_tag="ACHURS_004832"
                     /pseudo
     CDS             1..546
                     /locus_tag="ACHURS_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
BASE COUNT          159 a          128 c          144 g          115 t
ORIGIN      
        1 tgaaggcgct tcccgattat ctcttcctga aggcacttta ggacaatggg ttaccgccgc
       61 cagaaaaggg ctcggtactc ctggttcccg cacggtggct gaactggaat ctgaaattct
      121 gcaactgcgt aaggcgttaa atgaagctcg ccttgagcga gatatattaa aaaaagcaac
      181 agcgtatttt gcacaggagt cgctgaaaaa tacgcgttaa tcgaacaatg gcgacaacaa
      241 tttcccattg aagcgatgtg tcaggtattt ggtgtatcca ggagcggtta ttacaactgg
      301 gtacagcatg aaccctcaga cagaaaacaa agtgatgagc ggctaaaact ggagattaag
      361 gtggcacata tccgcactcg cgaaacatat ggaacccggc ggctccagac ggagctggca
      421 gagaatggca tcatcgttgg tcgtgaccga ctggcacgtc ttcgtaagga gctaaggcta
      481 cgctgtaagc agaaacgcaa gttcagagcg actacgaact cgaaccacaa tctgccagtt
      541 gcgcca
//
LOCUS       DAZPDU010000486          544 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZPDU010000486 DAZPDU010000000
VERSION     DAZPDU010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.492"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
BASE COUNT          152 a          113 c          132 g          147 t
ORIGIN      
        1 tctgcccttt aataatctac ggacagatta ctttctgtac ttccagagcc tgtttctgtg
       61 agtcaaacat gagctggttt ttaccgacta cacccaagga aatatattcg atatctccca
      121 tttgccgtat tttcgtaaaa ttcaacagga aatattcaac gggagtgaga gaactgctgt
      181 ccggggagga ggtacagacg caaaaaaacc gactggtgag gccggttttt ttgcgattcc
      241 ggatcgaagt tggtagccct gaccggaaga taactgccta taactgacta tgaaataata
      301 cggctgaaat aatatttctg caagcacagt ataagtatat actcggtgtg tcagctttga
      361 ggcacgttct ttccggcata tcgttcaccg ggatgaacag gttgcaagtc ctggccaaaa
      421 catcaggtgg tgctggtttt ggtgctctgg ccggagaaga actgtctata actgacacta
      481 tagaaaacgt ggcgacaggc aatgtaaggg caaaccacgg tgttctttct ccattgatgg
      541 agaa
//
LOCUS       DAZPDU010000487          539 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZPDU010000487 DAZPDU010000000
VERSION     DAZPDU010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.493"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            1..248
                     /gene="tnpA"
                     /locus_tag="ACHURS_004833"
                     /pseudo
     CDS             1..248
                     /gene="tnpA"
                     /locus_tag="ACHURS_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          168 a          131 c          106 g          134 t
ORIGIN      
        1 aaatggatgg cgaaccagat cacgtccatt tgttaattaa ctatcctccc aaactggcga
       61 tatccagtct ggtaaacagc ctcaaaggcg tatcgggtag gtgactgcga cgagatcgac
      121 cagatattgc agtcaggtat tactacaaag gcgttttgtg gagtgttggc tattttgcca
      181 gtagctgcgg aggtgcgcca atatccgtca tccgccaata cattgaacaa cagcaaacac
      241 ctggttaggt ggaaaaccac gccttatatc cctgccctga aggacggggt tttacggcgc
      301 accggataag cctggagatt atctacacca gcatggacat cattgtacta tgccccgtac
      361 atgccccatc acatcctcaa catcaaagta ttagagcctt gcaaaccatt atgcgtctca
      421 gtttgtctca tctatcttac gcaaaaaaaa tagaactaat atcaagcggc gctatattgc
      481 gtattaaata aaaccacctc aattacatta ataaatctaa acaaacatca tgcaaataa
//
LOCUS       DAZPDU010000488          536 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZPDU010000488 DAZPDU010000000
VERSION     DAZPDU010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.494"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..494)
                     /locus_tag="ACHURS_004834"
     CDS             complement(<1..494)
                     /locus_tag="ACHURS_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240332.1"
                     /translation="MKLNIFTKSMIGMGLVCSALPALAMEAWNNQQGGNKYQVIFDGK
                     IYENAWWGFSTNCPGKAKANDATNPWRLKRTATAAEISQFGNTLSCEKSGSSSSSNSN
                     TPASNTPANGGSATPAQGTVPSNSSVVAWNKQQGGQTWYVVFNGAVYKNAWWVASSNC
                     PGDA"
BASE COUNT          153 a          130 c          105 g          148 t
ORIGIN      
        1 ttcgcatcac ccggacagtt agaagaggct acccaccaag catttttata taccgcacca
       61 ttaaagacga cataccaggt ctgaccgccc tgctgtttat tccaggcaac tacagaagaa
      121 ttagacggaa cagtgccctg tgctggtgta gccgaaccgc cattagccgg cgtattggat
      181 gcaggcgtat ttgaatttga agaagatgag ctgccgctct tttcgcagga aagtgtattg
      241 ccaaactgac taatttcagc agctgttgcg gtacgcttta aacgccacgg gttagttgca
      301 tcatttgctt tcgcttttcc cgggcaattt gtagaaaaac cccaccaggc attttcataa
      361 attttgccat cgaaaataac ctgatattta ttaccacctt gttggttatt ccatgcttcc
      421 attgccaatg ctggcagagc ggaacacacc agccccatac caatcataga tttagtaaat
      481 atatttaatt tcattataat cccttgtgac gtaaaaactg caaaacaaaa gtccta
//
LOCUS       DAZPDU010000489          530 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZPDU010000489 DAZPDU010000000
VERSION     DAZPDU010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.495"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..333
                     /locus_tag="ACHURS_004835"
     CDS             <1..333
                     /locus_tag="ACHURS_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015387350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240333.1"
                     /translation="TSGSSNGSYSNLGSHRGSFTGRNTGSGTLFVYASGGNGGSAGGD
                     CANTSRLQGYVAGALISTNASNNPSYGKTAFISFAVPAGATYQITSYPAQNYSCGSGV
                     FSVFGYQT"
     gene            complement(330..>530)
                     /locus_tag="ACHURS_004836"
     CDS             complement(330..>530)
                     /locus_tag="ACHURS_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin"
                     /protein_id="HFR1240334.1"
                     /translation="ALGGKLKVTQLSSTGYLGQFDFCAIARMGNAEDSHYCQVVESPS
                     GSRKWYKYEHKTGCIASCVTLN"
BASE COUNT          135 a          125 c          122 g          148 t
ORIGIN      
        1 acttccggtt cctctaacgg tagttacagt aatcttggat cacaccgagg atcatttact
       61 ggtcgcaata cgggaagtgg tacgctgttt gtttacgcct ctggagggaa tggtggttcc
      121 gccgggggag attgtgctaa cacatccaga ttgcagggat acgttgcagg ggcattgatt
      181 agcacaaatg cgagtaataa cccatcttat gggaagacag cattcatctc ttttgctgtc
      241 cctgctggcg caacttatca gatcacctct tatccggccc aaaactactc ttgtggctca
      301 ggggtatttt cagtatttgg ataccaaact taattgagag ttacacagga cgcaatacaa
      361 cctgtcttgt gctcgtattt gtaccatttt cgtgaaccag atggactctc gactacctgg
      421 cagtaatgag aatcctcagc atttcccatt ctggcaatgg cacaaaagtc aaattgcccc
      481 aagtaaccag tgctggaaag ttgagtaacc ttgagctttc cacctaatgc
//
LOCUS       DAZPDU010000490          527 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZPDU010000490 DAZPDU010000000
VERSION     DAZPDU010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.496"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..275
                     /locus_tag="ACHURS_004837"
     CDS             <1..275
                     /locus_tag="ACHURS_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HFR1240335.1"
                     /translation="KMAQVIAKLLPLQLLRGVGLEQRQHVHLARKSLVEAVNADQIPD
                     LYGSRVPCLQATMHGLPVATRPKIKMVQLQFTPFASDDTYHWQTTM"
     gene            complement(254..>527)
                     /locus_tag="ACHURS_004838"
     CDS             complement(254..>527)
                     /locus_tag="ACHURS_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="shufflon protein D'"
                     /protein_id="HFR1240336.1"
                     /translation="RASGSTVLTGKIANGQQIPLPSGFSASQCTWSVSNAENPHGWKP
                     NYFAGSVATYDANRIVKCGFYDEYNFYGGTHRTDLSGKCSYIVVCQ"
BASE COUNT          141 a          131 c          121 g          134 t
ORIGIN      
        1 aaaaaatggc gcaggtgata gccaaattgt taccgcttca gctactgcgt ggcgttggcc
       61 tggagcaacg gcaacatgtc catctggcaa gaaagtcatt ggtggaggcg gtcaatgcag
      121 atcaaatacc ggatttatat ggctcacgcg ttccatgcct tcaggcaaca atgcatggac
      181 tgccagttgc gacacgaccg aagatcaaaa tggttcaatt acagtttacg ccatttgcca
      241 gtgacgatac ttatcactgg caaaccacga tgtaactaca tttccccgac aggtcagttc
      301 ggtgagtacc gccataaaag ttgtattcgt catagaagcc gcatttcaca attcgattgg
      361 catcataggt tgcaactgac cccgcaaaat aattcggctt ccacccgtgc ggattttctg
      421 cattactgac actccaggta cactggctgg cagaaaaacc agatggcaat gggatctgtt
      481 gtccatttgc tatttttcct gtcagaacag tgctacctga tgccctt
//
LOCUS       DAZPDU010000491          523 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZPDU010000491 DAZPDU010000000
VERSION     DAZPDU010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.497"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..159
                     /locus_tag="ACHURS_004839"
     CDS             <1..159
                     /locus_tag="ACHURS_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240337.1"
                     /translation="EFLFDNSLEKDENSSDNCFEVEKYNAYEAYNSENLYFTREEYTY
                     DYDLLNAI"
BASE COUNT          170 a          116 c           96 g          141 t
ORIGIN      
        1 gaattccttt ttgataatag cctcgagaaa gacgaaaatt cttcggacaa ttgctttgag
       61 gtcgaaaaat ataacgcata tgaagcatat aactcagaga atctatattt taccagagag
      121 gaatatacgt atgattacga ccttttaaat gcaatataga cataataact tcttacaaaa
      181 tctataatta ataccgtaga tattcattca tcttaccctg tgcaccaaaa gcaaaaactc
      241 actgacgcgg gtttgaaact gatggcaacc gcaaaaatgc ctgatgcgct acgcttatca
      301 ggcctacgcc atctctacaa tatattgaat ttgcgtgctt ttgtaggcag gataaggcgt
      361 tcacgcagca tccggcatga ataaagcgca ctcaacaatc tgaaacccgc cggaacggtt
      421 tattacgtac atcaggtgaa actgaccgat aagccgcttt cttttgggta tagtgtcgtg
      481 gacagtcatt catctttctg cccctccaaa agcaaaaacc cgc
//
LOCUS       DAZPDU010000492          523 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZPDU010000492 DAZPDU010000000
VERSION     DAZPDU010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.498"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..>523)
                     /locus_tag="ACHURS_004840"
     CDS             complement(<1..>523)
                     /locus_tag="ACHURS_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240338.1"
                     /translation="FLGVTTWIFMAAGGAESVAVYVNDVKGGSKSFVKVIILAGIFIG
                     VLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAYFGLPEALMNRFVGLVSFTAMFGS
                     LLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPARAAWIQFLIVIPLMIIPMLGSNT
                     VQDLMNTIINMTAA"
BASE COUNT          143 a          141 c          134 g          105 t
ORIGIN      
        1 gcggcggtca tattaataat agtattcatc agatcctgca cggtattgga accgagcatc
       61 gggataatca tcagcgggat gacgatcagg aactggatcc acgctgcgcg cgccggaacg
      121 ccgttttcgt tcagttcgac ggttttctta ccaaagatgc cttccgggat ttcggagaag
      181 aaaattttca ccggcgttgc ggtccacatc agcagggaac cgaacatcgc ggtaaaggac
      241 accagaccga caaagcgatt catcaacgct tccggtagac caaaatacgc cgccatgccg
      301 tggaatacct gcaccgatcc gccggtaaat ttcaactctt tgctgctgac gaagacgtta
      361 atcagcaccg aggagacgga atacagtacg ccgataaaaa ttccggcgag gatgatcact
      421 ttaacgaacg atttcgaacc gcctttgacg tcgttaacgt acacagcgac ggactccgcg
      481 ccacctgcgg ccataaagat ccaggtggta acgccgagga acg
//
LOCUS       DAZPDU010000493          521 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZPDU010000493 DAZPDU010000000
VERSION     DAZPDU010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.499"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..112)
                     /locus_tag="ACHURS_004841"
     CDS             complement(<1..112)
                     /locus_tag="ACHURS_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240339.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKF"
     gene            complement(368..>521)
                     /gene="tdcR"
                     /locus_tag="ACHURS_004842"
     CDS             complement(368..>521)
                     /gene="tdcR"
                     /locus_tag="ACHURS_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional activator TdcR"
                     /protein_id="HFR1240340.1"
                     /translation="IFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSSDRTSFC
                     ECNRFP"
BASE COUNT          161 a           99 c           99 g          162 t
ORIGIN      
        1 caaactttct ttttaccgtc caataatcaa tatttctatt gtgattgtta accactgcgt
       61 gcattggcgg gattgaaggg tgaaaaatat gtgcaattgg gaagcctttc attgatgcct
      121 gccttgtcgt catgacaaac aataatagat gcttattttc ctcgcagggt tctttcgcac
      181 tatcaaaaaa cgctcattta cccggcgtta cagtaaacga agcaacattc tgtggcctac
      241 gacgatcaaa agctctgtga aaatttccct tgttgtcttt gtctgacctt atcgcggcga
      301 tcagcaatta ttcgttttgt gatcctcaac acaaaatgct attcgtcgcc tatgagggga
      361 gaagaattta tggaaaacga ttgcattcac aaaatgacgt cctgtcagaa gagaaaaagt
      421 tgacgttaat caattgttcg agatgatcaa tacctgtatt cgaaatttta aaatattgat
      481 aaaataaatg tttgttctcc aacaaatagc gtgaaaaaat g
//
LOCUS       DAZPDU010000494          514 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZPDU010000494 DAZPDU010000000
VERSION     DAZPDU010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.500"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(116..424)
                     /locus_tag="ACHURS_004843"
                     /pseudo
     CDS             complement(116..424)
                     /locus_tag="ACHURS_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
BASE COUNT          139 a          132 c          125 g          118 t
ORIGIN      
        1 tgcgccagtg gcaatatgtg gcttagctca tgaaaggagc gcaacaagat gatgaatcaa
       61 catataacaa catcttaaaa aaaggcctga cattacgcca ggccttctgc gttaattaat
      121 cacgcttttc cagcagggtc cggtaaatca gaccaccgat aatgccgccg acaattggca
      181 ccacccagaa gaaccacagt tgttctaatg cccagccgcc ctggaagata gcaaccgcgg
      241 tgctgcgcgc cgggttaaca gaagtgttag tcaccggaat actaattaag tgaatcaggg
      301 ttaaggccag accaatagcg atcggcgcaa aacctgccgg cgcgaatttg tcggttgcgc
      361 cgtggatcac caacaggaaa cctgcactca ataccagttc aactaccagc gcggaggtga
      421 tgctgccaac ttactgattt agtgtatgat ggtgtttttg aggtgctcca gtggcttctg
      481 tttctatcag ctgtccctcc tgttcagcta ctga
//
LOCUS       DAZPDU010000495          510 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZPDU010000495 DAZPDU010000000
VERSION     DAZPDU010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.501"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..502
                     /locus_tag="ACHURS_004844"
     CDS             <1..502
                     /locus_tag="ACHURS_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HFR1240341.1"
                     /translation="ISDPNEPKWIPGHHISSNEVKKTENEVRQTEDKQHQNSEPEEGT
                     PCNQTGEDNCPDCGAVMGDATYQETFDEKKPDEAQEEEPKKTEKADDLLPENAGSDQH
                     NDSNNETGEDELNWKRQVLIAAVYGLCANPACIAYAPAIPDIAMMIANKLENFGVTSD
                     ERLPDF"
BASE COUNT          171 a          104 c          134 g          101 t
ORIGIN      
        1 gattagcgac ccgaatgaac cgaaatggat tcccggtcat catatctcct caaatgaagt
       61 caaaaaaacg gaaaatgaag tgcgacagac tgaagataaa cagcaccaga acagcgaacc
      121 ggaggaggga acaccctgca atcagactgg cgaggataac tgccctgact gtggcgcggt
      181 gatgggagac gcaacttatc aggaaacttt tgacgaaaaa aaaccagatg aagcacagga
      241 agaagagccg aagaaaacgg aaaaagctga tgatttgtta ccggagaacg ctggcagcga
      301 tcagcataat gatagcaata atgaaactgg cgaagatgaa ctcaactgga aaagacaggt
      361 gttgattgcg gcggtttacg gtttatgtgc gaatcctgcg tgcatagcct atgcgcctgc
      421 aatccctgac atcgcaatga tgattgccaa caagcttgaa aattttggag tgaccagtga
      481 tgagcgctta cccgattttt gatcgtattg
//
LOCUS       DAZPDU010000496          503 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZPDU010000496 DAZPDU010000000
VERSION     DAZPDU010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.502"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..227)
                     /locus_tag="ACHURS_004845"
     CDS             complement(<1..227)
                     /locus_tag="ACHURS_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240342.1"
                     /translation="MSGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGIT
                     YANPVNPVLGAKVLPGETDLALPCPLPFILFR"
BASE COUNT          124 a          149 c           96 g          134 t
ORIGIN      
        1 cggaaaagaa taaacggcag cgggcagggc agcgcaaggt ccgtctcgcc cggcagcacc
       61 ttcgcaccca gcaccgggtt caccgggtta gcataggtaa tccctcccgg acacaccgaa
      121 caggccactc ccgtcggcgc acctatcagc acacccgctg aaccctgcac aatatccagt
      181 cccttgcggg tcatgtcgcc ctgccgcgcc gccggttttc cgctcatggt ttattcctct
      241 tcatctgaat tgatttacat taggttggtt attttctcat acccacaact atattttctc
      301 tctgaattta ttacgtgcaa gacaatcgca gcactctaac taacttcgat gcaatttgat
      361 gttcagaaaa atccctcatt agattattat catgaaatac agctaatcat ctttagctta
      421 tatatttctc aatgtattct tttgaaagga tccgtgccct ttcaaactct tcagcagggc
      481 aagattacga aagcccgctc ccc
//
LOCUS       DAZPDU010000497          494 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZPDU010000497 DAZPDU010000000
VERSION     DAZPDU010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.503"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
BASE COUNT           90 a          162 c          141 g          101 t
ORIGIN      
        1 cgctacggtc cgatgtctga aaaaccaggc ttgcagcgac cgttccggtc gcgccgcccc
       61 tgtcgcgccg tgacctgcgc tccctgcaca cctgacggtg tgtgaggaaa ctgcgcctga
      121 tggcgcagca gtcctccctc ctgccgccac tcctgcggcc tgtttcccgc tgtggacttt
      181 ccggggggca gacgtaaccc cctcccgttc catctgtcca gggtgagatg caccggccac
      241 aaaattttta gccatgaacc cggcaaaaaa ttttgcgtcc gcccccggac agctgccccg
      301 tgaggcggta aacgggcccc catcggaaag gtccactgcg gggagaaacc cctgtgaagt
      361 ggacggcagg aggagctttc gctcctctga aggggcacat gaacgtcccg tcgccggtat
      421 ccggtttatc tcacatcttt cagtcaggag gttcttatga gtgcacgtgg tatcaacaag
      481 gtcatcctcg tcgg
//
LOCUS       DAZPDU010000498          489 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZPDU010000498 DAZPDU010000000
VERSION     DAZPDU010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.504"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..>489)
                     /gene="ipaH9.8"
                     /locus_tag="ACHURS_004846"
     CDS             complement(<1..>489)
                     /gene="ipaH9.8"
                     /locus_tag="ACHURS_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HFR1240343.1"
                     /translation="TGALLSLGREMFRLEILEDIARDKVRTLHFVDEIEVYLAFQTML
                     AEKLQLSTAVKEMRFYGVSGVTANDLRTAEAMVRSREENEFTDWFSLWGPWHAVLKRT
                     EADRWALAEEQKYEMLENEYPQRVADRLKASGLSGDADAEREAGAQVMRETEQQIYRQ
                     LTD"
BASE COUNT           99 a          153 c          117 g          120 t
ORIGIN      
        1 gtcagtcagc tgacggtaaa tctgctgttc agtctcacgc atcacctgtg caccggcttc
       61 cctctccgca tccgcatcac cgctcagacc tgatgctttc agccggtcag ccaccctctg
      121 agggtactca ttctccagca tctcatattt ctgctcttct gccagcgccc agcggtcagc
      181 ttccgtacgc ttcagtacag catgccatgg tccccagagg gagaaccagt ccgtaaattc
      241 attctcttca cggcttctga ccatggcttc ggcagtgcgg aggtcatttg ctgtcactcc
      301 cgacacgcca tagaaacgca tttccttcac ggcagtggag agctgaagtt tctctgcgag
      361 catggtctgg aaggccaggt agacttctat ctcatccaca aaatggagag ttctgacttt
      421 atcccgggca atgtcctcca gaatttcgag gcggaacatt tccctgccca gggagagcag
      481 agcgccggt
//
LOCUS       DAZPDU010000499          483 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZPDU010000499 DAZPDU010000000
VERSION     DAZPDU010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.505"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            58..462
                     /gene="stpA"
                     /locus_tag="ACHURS_004847"
     CDS             58..462
                     /gene="stpA"
                     /locus_tag="ACHURS_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFR1240344.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT          140 a          106 c          120 g          117 t
ORIGIN      
        1 gcttacacta cgcgacgaaa tacttttttg ttttggcgtt aaaaggtttt ctttattatg
       61 tccgtaatgt tacaaagttt aaataacatt cgcaccctcc gtgcgatggc tcgcgaattc
      121 tccattgacg ttcttgaaga aatgctcgaa aaattcaggg ttgtcactaa agaaagacgt
      181 gaagaagaag aacagcagca gcgtgaactg gctgagcgtc aggaaaaaat tagcacctgg
      241 ctggagctga tgaaggctga cggaattaac ccggaagagt tattgggtaa tagctctgct
      301 gctgcaccac gcgctggtaa aaaacgccag ccgcgtccgg cgaaatataa attcaccgat
      361 gttaacggtg aaactaaaac ctggaccggt cagggccgta caccgaagcc aattgctcag
      421 gcgctggcag aaggtaaatc tctcgacgat ttcctgatct aattactgag gccggatgtc
      481 gct
//
LOCUS       DAZPDU010000500          476 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZPDU010000500 DAZPDU010000000
VERSION     DAZPDU010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.506"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..>476
                     /locus_tag="ACHURS_004848"
     rRNA            <1..>476
                     /locus_tag="ACHURS_004848"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          119 a          115 c          142 g          100 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcac
//
LOCUS       DAZPDU010000501          469 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZPDU010000501 DAZPDU010000000
VERSION     DAZPDU010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.507"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..>469
                     /locus_tag="ACHURS_004849"
     rRNA            <1..>469
                     /locus_tag="ACHURS_004849"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          129 a           99 c          145 g           96 t
ORIGIN      
        1 tcctgactga ccgatagtga accagtaccg tgagggaaag gcgaaaagaa ccccggcgag
       61 gggagtgaaa aagaacctga aaccgtgtac gtacaagcag tgggagcacg cttaggcgtg
      121 tgactgcgta ccttttgtat aatgggtcag cgacttatat tctgtagcaa ggttaaccga
      181 ataggggagc cgaagggaaa ccgagtctta actgggcgtt aagttgcagg gtatagaccc
      241 gaaacccggt gatctagcca tgggcaggtt gaaggttggg taacactaac tggaggaccg
      301 aaccgactaa tgttgaaaaa ttagcggatg acttgtggct gggggtgaaa ggccaatcaa
      361 accgggagat agctggttct ccccgaaagc tatttaggta gcgcctcgtg aattcatctc
      421 cgggggtaga gcactgtttc ggcaaggggg tcatcccgac ttaccaacc
//
LOCUS       DAZPDU010000502          468 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZPDU010000502 DAZPDU010000000
VERSION     DAZPDU010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.508"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            85..444
                     /locus_tag="ACHURS_004850"
                     /pseudo
     CDS             85..444
                     /locus_tag="ACHURS_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
BASE COUNT          113 a          110 c          137 g          108 t
ORIGIN      
        1 gtggtgatgt ctatctcaca ccgatgaaca tgaccacgaa cccctccgat ggcagtaagg
       61 ccggtaagca gaaggataac gccaatgcag acgaaacaac gtcttgatgt accgctgagt
      121 ctgaaatctg tcagtgactc cggtgagttt gaagggtatg gctccgtctt tggtgtaaag
      181 gacagccacg atgatgtggt gatgtccggg gcatttgctg cttccctgcg ggcgtggagt
      241 gacagaaaag cgttacctgc gctgctctgg cagcaccgca tggatgaacc catcggtgtt
      301 tacaccgaaa tgaaggaaga cgatgtcggg ctttacgtca ggggacggtt gcttattgat
      361 gatgatcccc tcgcaaaacg tgcacatgca cacatgaagg ccggttcgtt aaccggcctt
      421 tctattgggt acgtcctgaa agactgaggt agcctgagtt taacggac
//
LOCUS       DAZPDU010000503          463 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZPDU010000503 DAZPDU010000000
VERSION     DAZPDU010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.509"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..>463
                     /locus_tag="ACHURS_004851"
     CDS             <1..>463
                     /locus_tag="ACHURS_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR1240345.1"
                     /translation="VKESNQRWCSDGFEFRCDNGEKLRVTFALDCCDREALHWAVTTG
                     GFNSETVQDVMLGAVERRFGNELPASPVEWLTDNGSCYRANETRQFARMLGLEPKSTA
                     VRSPESNGIAESFVKTIKRDYISVMPKPDGLTAAKNLAEAFEHYNEWHPHSA"
BASE COUNT          119 a          108 c          149 g           87 t
ORIGIN      
        1 gtgaaagaaa gtaatcagcg atggtgctct gacgggttcg agttccgctg tgataacgga
       61 gaaaaactgc gagtcacgtt cgcgctggac tgctgtgacc gtgaggcact gcactgggcg
      121 gtcactacgg gcggcttcaa cagtgaaaca gtacaggacg tcatgctggg agcggtggaa
      181 cgccgcttcg gcaacgagct tccggcgtct ccagtagagt ggctgacgga taatggttca
      241 tgctaccggg ctaatgaaac acggcagttt gcccggatgt tggggcttga accgaagagc
      301 acggcggtgc ggagtccgga gagtaacggc atagcagaga gcttcgtgaa aacgataaag
      361 cgtgactaca tcagtgtcat gcccaaacca gacgggttaa cggcagcaaa gaaccttgca
      421 gaggcgttcg agcattataa cgaatggcat ccgcatagtg cac
//
LOCUS       DAZPDU010000504          459 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZPDU010000504 DAZPDU010000000
VERSION     DAZPDU010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.510"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            2..388
                     /locus_tag="ACHURS_004852"
     CDS             2..388
                     /locus_tag="ACHURS_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR1240346.1"
                     /translation="MKGQVVELVRLSGRETLRQLEAKTGATRYLMSVLARELVASGDV
                     YNSGYGLFPSEQARKDWQNARKKLSRANLKKPSVVDPDLIWSLPDGEIRRYDRRQNII
                     CSECRKSEAMQRVLAFYQGNFQKVLL"
BASE COUNT          135 a           96 c          123 g          105 t
ORIGIN      
        1 aattaaaggg caggttgttg aacttgtacg tctgagcggt cgcgagacgt tgcggcaact
       61 ggaagccaag acaggtgcga caagatatct gatgagtgtt ctcgccagag agctggttgc
      121 cagtggcgat gtatacaact ctggttacgg gttattcccg tctgaacagg cgcgtaagga
      181 ctggcaaaac gcccgcaaaa aactctcaag ggcaaatctg aagaaaccat ctgtggttga
      241 tcctgacctt atctggtcat taccagacgg agaaatacgc cgctacgaca ggcgtcaaaa
      301 cataatctgt agcgagtgcc ggaagagcga agctatgcag cgtgtactgg ctttctatca
      361 gggtaatttt cagaaggtgc tgttgtgagc caaattaaca atcggaactt cgtgaagaga
      421 aagcataatc caaaatctga ataattaagt tcagcactg
//
LOCUS       DAZPDU010000505          452 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZPDU010000505 DAZPDU010000000
VERSION     DAZPDU010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.511"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..>452
                     /locus_tag="ACHURS_004853"
     CDS             <1..>452
                     /locus_tag="ACHURS_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR1240347.1"
                     /translation="GKMASWLVEWKNATETQWLKDAPSQPLQQSLKDLERAYKNFFQN
                     RAAFPRFKKRGQNDVFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQ
                     SCGKWYISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVN"
BASE COUNT          119 a          112 c          120 g          101 t
ORIGIN      
        1 cgggaaaatg gcttcctggc tggttgagtg gaaaaatgcc actgaaacgc aatggcttaa
       61 agatgccccg tcacagccat tgcaacagtc actgaaagac cttgagcggg cttacaaaaa
      121 cttcttccag aatcgggctg cttttccccg attcaaaaag cggggacaga atgatgtatt
      181 ccgctacccg cagggcgtta agctcgatca ggaaaacagc cgtatttttc tgccgaaact
      241 gggctggatg cgctaccgga acagccgtca ggtcacgggt gttgtgaaaa atgtcactgt
      301 cagccagtcc tgcggtaagt ggtacatcag tattcagaca gaaagtgaag tatcaactcc
      361 ggttcaccct tcagcatcaa tggtcgggct ggatgctggc gtggctaaac tcgccacgct
      421 gtcagatggc acagtctttg agcctgtaaa ca
//
LOCUS       DAZPDU010000506          451 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZPDU010000506 DAZPDU010000000
VERSION     DAZPDU010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.512"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            75..>451
                     /locus_tag="ACHURS_004854"
     CDS             75..>451
                     /locus_tag="ACHURS_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFR1240348.1"
                     /translation="MTKDELIARLQELGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVVSAQPDTVIQDTAELVTVVALVTLHTDALHATRDK
                     PVAFVLPGTAFRVSAGVAAEMTER"
BASE COUNT          101 a          106 c          145 g           99 t
ORIGIN      
        1 cctgatgagt tcgtgtccgt tcaactggcg taatcatggc ccttcggggc cattttctct
       61 ctgtggagga gttcatgacg aaagatgaac tgattgcccg tcttcaggag ctgggtgagc
      121 aattgaaccg cgatgtcagt ttgacgggaa cgaaagaaga actggcgctt cgtgtggcag
      181 agctggaaga agagcttgat gacacggatg aaactgccgg tcaggacacc cctctcagcc
      241 gggaaaatgt gctgaccggg catgaaaatg aggtggtatc agcgcagccg gataccgtga
      301 ttcaggatac ggctgaactg gtcacggtcg tggcactggt gacgctgcat actgatgcac
      361 ttcacgccac gcgggataaa cctgtggcat ttgtgctgcc gggaacggcg tttcgtgtct
      421 ctgccggtgt ggcagccgaa atgacagagc g
//
LOCUS       DAZPDU010000507          439 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZPDU010000507 DAZPDU010000000
VERSION     DAZPDU010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.513"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..103
                     /locus_tag="ACHURS_004855"
     CDS             <1..103
                     /locus_tag="ACHURS_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFR1240349.1"
                     /translation="ECEKMLSGEKEAGASREQKVKLINFNYEKLQSS"
     gene            210..>439
                     /locus_tag="ACHURS_004856"
     CDS             210..>439
                     /locus_tag="ACHURS_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFR1240350.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSP"
BASE COUNT          151 a           90 c           97 g          101 t
ORIGIN      
        1 agaatgcgaa aaaatgctct caggggaaaa agaagcaggc gcttccagag agcagaaagt
       61 aaaactgata aattttaatt acgagaaact tcagtcctcg tgaatttcat tatatccaga
      121 ttagaacttc atagctgatt attaaaaatc aaccacaccc gccagtattc tgtatattta
      181 ctggcggtca tatcgtgaga ggtatggcaa tgaatcttgt gacactcaaa acgtggggaa
      241 aactcagata tccggataac ccaccatcaa tatcaacgct gagacgatgg gcaaggaatg
      301 gaaacattta tcctgcacct gaactacacg ggaggagtta cagggtggtt ccggaggctt
      361 tctatatcaa cccaaataag gttgataccg atataacaca ccatcagcct aatgggcgac
      421 aagggagaga cagtccgtt
//
LOCUS       DAZPDU010000508          439 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZPDU010000508 DAZPDU010000000
VERSION     DAZPDU010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.514"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..>439
                     /locus_tag="ACHURS_004857"
     CDS             <1..>439
                     /locus_tag="ACHURS_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR1240351.1"
                     /translation="WHIAANIQFPPNFDESKQYPAIISVHPFGSCKEQTSGNIYGKAL
                     AEKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLPYVNAERIGVLGV
                     CGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGA"
BASE COUNT          109 a           85 c          123 g          122 t
ORIGIN      
        1 tggcatattg ctgccaatat tcagtttcca ccaaattttg atgagtcaaa acagtatccg
       61 gcgattatca gcgtccatcc ttttggcagt tgtaaggagc agacgtctgg caatatctat
      121 ggcaaggcat tggctgaaaa aggctatctc gttctcgctt atgacgccag tttccaggga
      181 gaatctggtg gggagcctcg ctggatagaa gatccgacac agcgcgtaga ggatattagc
      241 cgtgttatcg attatgcagt aactctgcca tatgttaatg ctgaacgtat tggcgtgctg
      301 ggagtgtgtg ggggtggtgg ctatgccatt aatgccgcgc tgacagaaaa acgaattaaa
      361 gcagtagtga gtattacggg tgttaatatc gggcgtcttt ttcgggaagg tttcagcaat
      421 tacgatccga ttggcgcat
//
LOCUS       DAZPDU010000509          420 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZPDU010000509 DAZPDU010000000
VERSION     DAZPDU010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.515"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            41..116
                     /locus_tag="ACHURS_004858"
     tRNA            41..116
                     /locus_tag="ACHURS_004858"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:74..76,aa:Ile,seq:cat)
BASE COUNT          117 a           79 c           98 g          126 t
ORIGIN      
        1 tttattgcgt ggtttataag tattgttatt gcacactcaa ggccctttag ctcagtggtt
       61 agagcaggcg actcataatc gcttggtcgc tggttcaagt ccagcaaggg ccaccaaatc
      121 attatttacg tatcaggaag agttaagggt acaaggggta ctcgatggcg gtctatattt
      181 catgactaac gccatttcaa ggaagaagtg cggtgggtgg tttcgaatga gggctggtgt
      241 tttggtacgt ggcacgccac tgcaatgtat ttatccatca gtactcattc aaaagtacct
      301 ggaaaaaatg cctgctatgc caagcgggca aattgcttac atgtggaatt aagtaattaa
      361 cgataaagcc agagtattat catttattat accccttcat ttaatcaggt taattactca
//
LOCUS       DAZPDU010000510          399 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZPDU010000510 DAZPDU010000000
VERSION     DAZPDU010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.516"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..>399
                     /locus_tag="ACHURS_004859"
     CDS             <1..>399
                     /locus_tag="ACHURS_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HFR1240352.1"
                     /translation="QSKSLGESLGGLMSDMSHGVDSLTDISYRVRAFFDEVTLAYRSL
                     NDTQKAVADGLANGLLSALKGLAGIVAVRSGIGAVGGIWNLIRAISTLGNVANTAAGR
                     INSRSTGSVEGGKSRLSVADAITRVMIVGMT"
BASE COUNT          105 a           65 c          110 g          119 t
ORIGIN      
        1 tcaatctaaa agtcttggtg aatctcttgg tggcttgatg agcgatatgt cgcatggggt
       61 cgattcacta accgatatta gttacagggt acgagcgttc tttgatgagg tgacattagc
      121 ttatcgttca ctaaatgaca ctcaaaaagc tgtagctgat ggtcttgcaa atggcctgtt
      181 aagtgcatta aaggggcttg ctggtatagt cgctgtacgg tctgggattg gtgctgtagg
      241 tggtatctgg aacttaatta gggccatttc cacgcttgga aatgtggcaa atacagcagc
      301 aggaagaatc aattcacgtt ctactggtag tgttgaaggt ggaaaaagta ggctatctgt
      361 agctgatgca attaccagag taatgattgt cggtatgac
//
LOCUS       DAZPDU010000511          395 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZPDU010000511 DAZPDU010000000
VERSION     DAZPDU010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.517"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..>395
                     /locus_tag="ACHURS_004860"
     CDS             <1..>395
                     /locus_tag="ACHURS_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFR1240353.1"
                     /translation="LVVDQPATIGALPVAVARSEGVLVGYLPGLAMRRIADLHAGEAK
                     TDARDAAIIAEAARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIRGLL
                     TQIHPALERVLGPRLEHPAVLDLLQRYPSP"
BASE COUNT           76 a          137 c          100 g           82 t
ORIGIN      
        1 ctggttgttg atcagccagc taccatcggt gcgttacctg tcgccgttgc ccgctcagaa
       61 ggagtccttg tcggatacct ccctggactg gccatgcgcc gcatagccga cttacacgcc
      121 ggtgaagcta aaactgatgc tcgtgacgct gccatcattg ccgaagctgc ccgtaccctg
      181 cctcacgcgc tacgcacgct gaaactggct gacgagcaaa tcgccgaact ctccatgctc
      241 tgcggcttcg atgatgatct tgccgcacag acaacgcagg ccagcaaccg tatccgcggc
      301 cttctgaccc agatacatcc ggcactggag cgcgttctcg gtccgagact tgagcacccg
      361 gcggtactcg atcttctcca gcgatatccc tcacc
//
LOCUS       DAZPDU010000512          368 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.520, whole genome shotgun sequence.
ACCESSION   DAZPDU010000512 DAZPDU010000000
VERSION     DAZPDU010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.520"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..178
                     /locus_tag="ACHURS_004861"
     CDS             <1..178
                     /locus_tag="ACHURS_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR1240354.1"
                     /translation="LMQVASEHIAPLQDAVDLEIATEKETLLLEAWKKYRVLLNRVDT
                     STAPDIEWPVAPIG"
BASE COUNT          120 a           58 c           94 g           96 t
ORIGIN      
        1 cctgatgcag gtagccagtg agcatatagc gccgcttcag gatgctgtgg atctggagat
       61 cgcaacggag aaagaaacct tgttgctgga agcctggaaa aagtatcggg tgttgctgaa
      121 ccgtgttgat acatcaactg caccggatat tgaatggcca gtagcaccta tagggtaaat
      181 tcgtaatgat tacctaaata cgttattctt ttgttaaaaa agtgattttg ctataggtag
      241 ggagtatgtt cttggattaa gaataaaaaa tcgccatgaa agagcttcat ggcgagtaca
      301 taaacgtaca aaatgtgatg aaagtagagt ggcgtaagcc actatattaa gatatgacaa
      361 tagcgaag
//
LOCUS       DAZPDU010000513          357 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.521, whole genome shotgun sequence.
ACCESSION   DAZPDU010000513 DAZPDU010000000
VERSION     DAZPDU010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.521"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..>357
                     /locus_tag="ACHURS_004862"
     rRNA            <1..>357
                     /locus_tag="ACHURS_004862"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           95 a           82 c          105 g           75 t
ORIGIN      
        1 taaaaacaca gcactgtgca aacacgaaag tggacgtata cggtgtgacg cctgcccggt
       61 gccggaaggt taattgatgg ggtcagcgca agcgaagctc ttgatcgaag ccccggtaaa
      121 cggcggccgt aactataacg gtcctaaggt agcgaaattc cttgtcgggt aagttccgac
      181 ctgcacgaat ggcgtaatga tggccaggct gtctccaccc gagactcagt gaaattgaac
      241 tcgctgtgaa gatgcagtgt acccgcggca agacggaaag accccgtgaa cctttactat
      301 agcttgacac tgaacattga gccttgatgt gtaggatagg tgggaggctt tgaagtg
//
LOCUS       DAZPDU010000514          354 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.522, whole genome shotgun sequence.
ACCESSION   DAZPDU010000514 DAZPDU010000000
VERSION     DAZPDU010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.522"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            216..>354
                     /locus_tag="ACHURS_004863"
     CDS             216..>354
                     /locus_tag="ACHURS_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="activates the csgBA and csgDEFG operons involved in
                     biofilm formation; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HFR1240355.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RS"
BASE COUNT          104 a           64 c           64 g          122 t
ORIGIN      
        1 tataacgagt tgcatttagt tacatgttta acacttgatt taagatttgt aatggctaga
       61 ttgaaatcag atgtaatcca ttagttttat attttaccca tttagggctg atttattact
      121 acacacagca gtgcaacatc tgtcagtact tctggtgctt ctattttaga ggcagctgtc
      181 aggtgtgcga tcaataaaaa aagcggggtt tcatcatgtt taatgaagtc catagtattc
      241 atggtcatac attattgttg atcactaaac cttctttgca ggcgacggct ctcttgcagc
      301 accttaaaca atcgctggca attacaggaa aattacataa tattcaacgt tctc
//
LOCUS       DAZPDU010000515          348 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.524, whole genome shotgun sequence.
ACCESSION   DAZPDU010000515 DAZPDU010000000
VERSION     DAZPDU010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.524"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..163
                     /gene="traL"
                     /locus_tag="ACHURS_004864"
     CDS             <1..163
                     /gene="traL"
                     /locus_tag="ACHURS_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFR1240356.1"
                     /translation="IGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVP
                     DSCFRQWIK"
     gene            185..>348
                     /locus_tag="ACHURS_004865"
     CDS             185..>348
                     /locus_tag="ACHURS_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraE/TraK family type IV conjugative transfer
                     system protein"
                     /protein_id="HFR1240357.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNLIQGVNNYRLQNE
                     QRTAVTPMGF"
BASE COUNT           83 a           73 c           84 g          108 t
ORIGIN      
        1 tattggtgca gcggttctgg tttatttcgg gattaaaaaa ctgaaaaaag ggcggggcag
       61 ttcctggtta cgtgacctga tttactggta tatgccaaca gccctgctgc gcggtatttt
      121 tcataatgtt cccgattcgt gtttccggca gtggattaaa tagaactgat accaggattg
      181 ttatatggaa cacggtgccc gtttaagtac cagtcgtgta atggccatcg cctttatatt
      241 tatgtcagtg cttattgttc tcagcctctc tgttaacctc attcaggggg tgaataacta
      301 ccgtcttcag aatgagcaac gcactgccgt gaccccaatg ggatttaa
//
LOCUS       DAZPDU010000516          332 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.525, whole genome shotgun sequence.
ACCESSION   DAZPDU010000516 DAZPDU010000000
VERSION     DAZPDU010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.525"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            1..332
                     /locus_tag="ACHURS_004866"
                     /pseudo
     CDS             1..332
                     /locus_tag="ACHURS_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT           85 a           83 c           99 g           65 t
ORIGIN      
        1 gctatgcagc gaactgcata ttgccccgtc aacgtattac cactgtcagc aacagcgaca
       61 tcatccggat aaacgcagtg cccgtgcgca gcgcgatgac tggctgaaga aagagataca
      121 gcgcgtatac gatgaaaatc acaaggtata cggtgtaaag tctggcgtca gttgttacgg
      181 gaaggtatca gagtggccag atgcactgtg gcacgtctca tggcggttat gggacttgcc
      241 ggtgttctcc ggggtaaaaa ggtccgtacg accatcagcc ggaaagccgt tgccgcaggc
      301 gaccgcgtaa accgtcagtt cgtggcagaa cg
//
LOCUS       DAZPDU010000517          332 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.526, whole genome shotgun sequence.
ACCESSION   DAZPDU010000517 DAZPDU010000000
VERSION     DAZPDU010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.526"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(30..279)
                     /gene="ymgC"
                     /locus_tag="ACHURS_004867"
                     /pseudo
     CDS             complement(30..279)
                     /gene="ymgC"
                     /locus_tag="ACHURS_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgC"
BASE COUNT          112 a           63 c           57 g          100 t
ORIGIN      
        1 tctggagatt cagggggcca gtctaagttc taagagagca cggattccct gtcattaata
       61 atgatatccg ttagtgtagt cttatgcatt tcaatgtact gcaatatttg cagatagaga
      121 tcagaaagaa tcacactggt ttcatctttt aaacgcaagc gtaaagcctg atcaataata
      181 tgactatcaa catacccatg cgtcatgaac acctccctct cgagattttt aaacaaagca
      241 cattgaaaaa ataaatctct ctgggattga attattcatg tcagcctcgt agaaatatta
      301 acctaataaa attatgctgt gtatagtcta aa
//
LOCUS       DAZPDU010000518          317 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.527, whole genome shotgun sequence.
ACCESSION   DAZPDU010000518 DAZPDU010000000
VERSION     DAZPDU010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.527"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..242)
                     /locus_tag="ACHURS_004868"
     CDS             complement(<1..242)
                     /locus_tag="ACHURS_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFR1240358.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT           91 a           66 c           73 g           87 t
ORIGIN      
        1 ttgatcgaca aatccatcaa tttatggacg ttttcgtcgt cccaggtctg gcagaaagtg
       61 gccaggttct gacgagcgtt gccatcaaga tataattcat cattgataat ctggaatgcg
      121 acatcatcgc gcatttcgtg cagcggaaat cgttttgact ccgcgatagt agaaatggcc
      181 tttgcgccaa aacgtgaatc gagtagttct gagcggaaat ccgttaacag cttctggtcc
      241 atttcgaact ccttaaattt atttgaaggc aataaaaaag taggatttat ccgcaatgga
      301 agcaaggcat tacaggc
//
LOCUS       DAZPDU010000519          309 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.529, whole genome shotgun sequence.
ACCESSION   DAZPDU010000519 DAZPDU010000000
VERSION     DAZPDU010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.529"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..>309
                     /locus_tag="ACHURS_004869"
     CDS             <1..>309
                     /locus_tag="ACHURS_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFR1240359.1"
                     /translation="EQRVLAHPLYPVLTSMPGVGVRTAARLLTEVACRAFASAAHLAA
                     YAGLAPVTRRSGSSIRGEHPSRRGNKALKRALFLSAFAALRDPLSRAYYTRKMSQGKR
                     "
BASE COUNT           56 a          107 c           83 g           63 t
ORIGIN      
        1 agaacagcga gttcttgctc accctcttta cccggtcctg accagtatgc ccggagtcgg
       61 tgtcaggacc gcagccagac tcctcaccga ggtcgcctgc cgcgccttcg cctctgccgc
      121 acatctcgct gcttatgctg gccttgcgcc ggtaactcgg cgatccggct cgtcaatacg
      181 cggtgagcat ccctcgcgac ggggtaataa agctctcaaa cgggcgttgt tcctgtcggc
      241 cttcgccgcg ctcagggatc cgctctccag ggcttactac acccgcaaaa tgagtcaggg
      301 aaaacgaca
//
LOCUS       DAZPDU010000520          308 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.530, whole genome shotgun sequence.
ACCESSION   DAZPDU010000520 DAZPDU010000000
VERSION     DAZPDU010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.530"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..>308
                     /locus_tag="ACHURS_004870"
     CDS             <1..>308
                     /locus_tag="ACHURS_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFR1240360.1"
                     /translation="EYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEACML
                     GGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQLFMD
                     P"
BASE COUNT           67 a           82 c           92 g           67 t
ORIGIN      
        1 gaatatccgc aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg
       61 catgcgcctg cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag
      121 gcctgtatgc tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca
      181 ggcaaaccaa cggataaacc aaacctggtg tgcggtccgg tacaaatctg ctggcataaa
      241 ttcgcccgct actgggatgt ggagctgcgt gagatcccta tgcgccccgg tcagttgttt
      301 atggaccc
//
LOCUS       DAZPDU010000521          301 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.531, whole genome shotgun sequence.
ACCESSION   DAZPDU010000521 DAZPDU010000000
VERSION     DAZPDU010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.531"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..>301
                     /locus_tag="ACHURS_004871"
     CDS             <1..>301
                     /locus_tag="ACHURS_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240361.1"
                     /translation="DISHLSEGHRVTVHYGYDEKGRLTGERQTVHHPQTEALLWQHET
                     RHAYNAQGLANRCIPDSLSAVEWLTYGSGWLSGMKLGDTPLVEYTRDRLHRETLRS"
BASE COUNT           67 a           91 c          101 g           42 t
ORIGIN      
        1 gacatcagcc atctcagcga agggcaccgg gtgacggtgc attacgggta tgatgagaaa
       61 ggccggctga ccggtgagcg tcagacggtg catcacccgc agacggaagc actgctctgg
      121 cagcatgaga ccagacatgc gtacaacgcg caggggctgg cgaaccgctg tataccggac
      181 agcctgtccg ccgtggaatg gctgacctac ggcagcggct ggctgtcagg catgaaactc
      241 ggcgacacac cgctggtgga gtacacccgc gaccgcctgc accgggaaac gctgcgcagc
      301 t
//
LOCUS       DAZPDU010000522          295 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.532, whole genome shotgun sequence.
ACCESSION   DAZPDU010000522 DAZPDU010000000
VERSION     DAZPDU010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.532"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..>295
                     /locus_tag="ACHURS_004872"
     CDS             <1..>295
                     /locus_tag="ACHURS_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HFR1240362.1"
                     /translation="DRGPQSLRCLQLLEQHWVCAVRGNHEQMAMDALASQQMSLWLMN
                     GGDWFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQ"
BASE COUNT           79 a           57 c           81 g           78 t
ORIGIN      
        1 cgatcgtggg ccgcaaagtt tacgttgtct gcagttactg gaacaacatt gggtttgtgc
       61 ggtaagaggc aatcatgaac agatggcgat ggatgcgctg gcatcccagc agatgtcttt
      121 gtggttgatg aatggcggcg actggtttat tgcgctggca gataatcaac agaaacaagc
      181 gaaaacggcg ctggaaaaat gtcagcattt gccctttatt cttgaagtac acagtcgtac
      241 cggcaaacat gttattgctc atgccgatta tccagatgat gtttatgaat ggcaa
//
LOCUS       DAZPDU010000523          295 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.533, whole genome shotgun sequence.
ACCESSION   DAZPDU010000523 DAZPDU010000000
VERSION     DAZPDU010000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.533"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..279
                     /locus_tag="ACHURS_004873"
     CDS             <1..279
                     /locus_tag="ACHURS_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HFR1240363.1"
                     /translation="LTYYADDGWAEADNNIAENALRMVSLGRKNYLFFGSDHGGERGA
                     LLYSLIGTCKLNGVEPESYLRYVLDVIADWPINRVGELLPWRVALPTE"
BASE COUNT           70 a           77 c           89 g           59 t
ORIGIN      
        1 ctgacgtact atgcagatga tggctgggct gaggcggaca ataacatcgc tgaaaatgcg
       61 ttgcggatgg tcagtctggg ccgcaaaaac tacctgttct tcggttcgga tcatggagga
      121 gagcggggag cgctgctgta cagcctgatc gggacgtgca aactgaacgg agtggagcca
      181 gaaagctacc tccgctatgt ccttgacgtc atagccgact ggccgataaa ccgggtcggc
      241 gaactgctcc cctggcgcgt agcactgccg actgaataac acattcccgt caata
//
LOCUS       DAZPDU010000524          291 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.534, whole genome shotgun sequence.
ACCESSION   DAZPDU010000524 DAZPDU010000000
VERSION     DAZPDU010000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.534"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..>291
                     /gene="traA"
                     /locus_tag="ACHURS_004874"
     CDS             <1..>291
                     /gene="traA"
                     /locus_tag="ACHURS_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HFR1240364.1"
                     /translation="TRLNMLRLVRAVIPVAVLMMLFPELAMAAGKGTDLMAKGQETVK
                     ATFGKDSSIVKWVVLAEVLVGAVMYMMTKNVKFLVGFAILSVFIAVGMSVAGF"
BASE COUNT           58 a           60 c           82 g           91 t
ORIGIN      
        1 actcgtctga atatgcttcg cctggttcgt gcagtgatcc cggttgctgt tctgatgatg
       61 ttgttccctg agttagctat ggctgctggt aaaggcacgg acctgatggc taaaggtcag
      121 gaaacggtta aggcgacctt cggtaaggac tccagtatcg ttaaatgggt tgttctggct
      181 gaagttctgg tcggtgcagt catgtacatg atgaccaaaa acgtcaagtt cctggtcggc
      241 tttgccatac tctctgtatt tattgcagta ggtatgtctg ttgccggatt c
//
LOCUS       DAZPDU010000525          291 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.535, whole genome shotgun sequence.
ACCESSION   DAZPDU010000525 DAZPDU010000000
VERSION     DAZPDU010000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.535"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..>291
                     /locus_tag="ACHURS_004875"
     CDS             <1..>291
                     /locus_tag="ACHURS_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HFR1240365.1"
                     /translation="QGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRSLKN
                     EWMPVVGYVSFSEAAHAITDYIVGYYSALRPHEYNGGLPPNESENRYWKNSN"
BASE COUNT           87 a           66 c           80 g           58 t
ORIGIN      
        1 atcagggcag tcattatacg agcaggcagt tccggcagtt attgtggcga taccagatca
       61 gacagagtat gagccggcgc ggaaactgct gggataacag cccaatggaa cgcttcttca
      121 ggagtctgaa gaacgaatgg atgccggtgg tgggttacgt aagcttcagc gaggcagctc
      181 acgccataac ggactatatc gttggatatt acagcgcact aagaccgcac gaatataacg
      241 gtgggttacc cccaaacgaa tcggaaaatc gatactggaa aaactctaac t
//
LOCUS       DAZPDU010000526          287 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.536, whole genome shotgun sequence.
ACCESSION   DAZPDU010000526 DAZPDU010000000
VERSION     DAZPDU010000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.536"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..>287
                     /locus_tag="ACHURS_004876"
     CDS             <1..>287
                     /locus_tag="ACHURS_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFR1240366.1"
                     /translation="RLISGKTQNDQDICALAKEYEIAMLLVTKGAEGVVVCYRGQVHH
                     FAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRIIDLAQRCGAL"
BASE COUNT           72 a           55 c           89 g           71 t
ORIGIN      
        1 gcgacttatc agtggaaaaa cacagaacga tcaggatata tgcgccctgg caaaagagta
       61 tgagatcgcc atgctgttgg tgactaaagg tgcagaaggg gtggtggtct gttatcgagg
      121 acaagttcac cattttgctg gaatgtctgt gaattgtgtc gatagcacgg gggcgggaga
      181 tgcgttcgtt gccgggttac tcacaggtct gtcctctacg ggattatcta cagatgagag
      241 agaaatgcga cgaattatcg atctcgctca acgttgcgga gcgcttg
//
LOCUS       DAZPDU010000527          286 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.denovo.537, whole genome shotgun sequence.
ACCESSION   DAZPDU010000527 DAZPDU010000000
VERSION     DAZPDU010000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.denovo.537"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
BASE COUNT           97 a           67 c           58 g           64 t
ORIGIN      
        1 ctgtacaaaa aaccgccagc caaggcagtt aagagtgtgt tcccggggtt tgcttagaat
       61 attttgtaag ttgtccgaag agatatttac aacaccagaa tgatgaatca tcagtcccct
      121 gccagaggtt cactacgtaa cctgaccgac aatgcaccat tctggtgctg aaaaaagagc
      181 actggaactg caacaaaaca acatcacaga acaggagatg gtaaggagtc agacattccc
      241 acacaatatt gtgtcagtgc aaataacaac cttcgtctca aatctc
//
LOCUS       DAZPDU010000528        21432 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.guided.062, whole genome shotgun sequence.
ACCESSION   DAZPDU010000528 DAZPDU010000000
VERSION     DAZPDU010000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21432
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.guided.062"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(34..204)
                     /locus_tag="ACHURS_004877"
                     /pseudo
     CDS             complement(34..204)
                     /locus_tag="ACHURS_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(423..1400)
                     /gene="epmA"
                     /locus_tag="ACHURS_004878"
     CDS             complement(423..1400)
                     /gene="epmA"
                     /locus_tag="ACHURS_004878"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFR1240367.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1725..3533
                     /gene="frdA"
                     /locus_tag="ACHURS_004879"
     CDS             1725..3533
                     /gene="frdA"
                     /locus_tag="ACHURS_004879"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFR1240368.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3526..4260
                     /gene="frdB"
                     /locus_tag="ACHURS_004880"
     CDS             3526..4260
                     /gene="frdB"
                     /locus_tag="ACHURS_004880"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFR1240369.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4271..4666
                     /gene="frdC"
                     /locus_tag="ACHURS_004881"
     CDS             4271..4666
                     /gene="frdC"
                     /locus_tag="ACHURS_004881"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFR1240370.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4677..5036
                     /gene="frdD"
                     /locus_tag="ACHURS_004882"
     CDS             4677..5036
                     /gene="frdD"
                     /locus_tag="ACHURS_004882"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFR1240371.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5099..6232
                     /locus_tag="ACHURS_004883"
     CDS             5099..6232
                     /locus_tag="ACHURS_004883"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFR1240372.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6321..6854
                     /gene="blc"
                     /locus_tag="ACHURS_004884"
     CDS             6321..6854
                     /gene="blc"
                     /locus_tag="ACHURS_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFR1240373.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6851..7168)
                     /gene="sugE"
                     /locus_tag="ACHURS_004885"
     CDS             complement(6851..7168)
                     /gene="sugE"
                     /locus_tag="ACHURS_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFR1240374.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7343..7489)
                     /gene="ecnB"
                     /locus_tag="ACHURS_004886"
     CDS             complement(7343..7489)
                     /gene="ecnB"
                     /locus_tag="ACHURS_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFR1240375.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7600..7725)
                     /gene="ecnA"
                     /locus_tag="ACHURS_004887"
     CDS             complement(7600..7725)
                     /gene="ecnA"
                     /locus_tag="ACHURS_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFR1240376.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(7777..8343)
                     /gene="efp"
                     /locus_tag="ACHURS_004888"
     CDS             complement(7777..8343)
                     /gene="efp"
                     /locus_tag="ACHURS_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFR1240377.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8385..9413
                     /gene="epmB"
                     /locus_tag="ACHURS_004889"
     CDS             8385..9413
                     /gene="epmB"
                     /locus_tag="ACHURS_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFR1240378.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPGELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPILRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            9803..10672
                     /locus_tag="ACHURS_004890"
     CDS             9803..10672
                     /locus_tag="ACHURS_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFR1240379.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLNAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGTVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(10875..11228)
                     /locus_tag="ACHURS_004891"
     CDS             complement(10875..11228)
                     /locus_tag="ACHURS_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFR1240380.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(11366..13012)
                     /gene="groL"
                     /locus_tag="ACHURS_004892"
     CDS             complement(11366..13012)
                     /gene="groL"
                     /locus_tag="ACHURS_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFR1240381.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(13056..13349)
                     /locus_tag="ACHURS_004893"
     CDS             complement(13056..13349)
                     /locus_tag="ACHURS_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFR1240382.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            13625..14881
                     /gene="yjeH"
                     /locus_tag="ACHURS_004894"
     CDS             13625..14881
                     /gene="yjeH"
                     /locus_tag="ACHURS_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFR1240383.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAAQVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LTCFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(14903..15373)
                     /locus_tag="ACHURS_004895"
     CDS             complement(14903..15373)
                     /locus_tag="ACHURS_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFR1240384.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDR"
     gene            15710..17146
                     /gene="aspA"
                     /locus_tag="ACHURS_004896"
     CDS             15710..17146
                     /gene="aspA"
                     /locus_tag="ACHURS_004896"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFR1240385.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            17264..18565
                     /gene="dcuA"
                     /locus_tag="ACHURS_004897"
     CDS             17264..18565
                     /gene="dcuA"
                     /locus_tag="ACHURS_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFR1240386.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            18681..19019
                     /gene="cutA"
                     /locus_tag="ACHURS_004898"
     CDS             18681..19019
                     /gene="cutA"
                     /locus_tag="ACHURS_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFR1240387.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            18995..20692
                     /gene="dsbD"
                     /locus_tag="ACHURS_004899"
     CDS             18995..20692
                     /gene="dsbD"
                     /locus_tag="ACHURS_004899"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFR1240388.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSHFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTHLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGMLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPIFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKKFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            20729..21304
                     /locus_tag="ACHURS_004900"
     CDS             20729..21304
                     /locus_tag="ACHURS_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1240389.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
BASE COUNT         5283 a         5522 c         5365 g         5262 t
ORIGIN      
        1 ccatcataca ctaaatcagt aagttggcag catcaccatt aaggcgaatg gaataaagaa
       61 taacaatgca gaaaatatat aaaagggaat cgcactataa cccattaagt aataagccga
      121 tgggctattg gcaaatccaa atacggaagt aaagatcatc aatatgagtc ctatcagact
      181 catctttttt atcgtgtgag gcatgaaacc atccttcatt tcagcgcgtg tataacacgt
      241 gcccactgaa ctgcactgcg caacattgag caggcagcgt aaaaaccaat acagaattga
      301 tacccggatg atatcaaata tccagtctta aattgtggcc tttaacggaa aaatgaaaac
      361 atatgctaca gaatggcgct tatcacgcca ttcttcgctg ttaattcagt aatttttcag
      421 aattatgccc ggtcaacgct aaaggcgatg acttcagcca gtgtctccgc gcccagcgcc
      481 aacatcacca gacgatcaac acctaatgcc acgccggaac agtcaggcat accgactttc
      541 aaggcttcaa tcagattctg gtcaatgggg tgctgcggca aaccgcgcgc cgcgcgctta
      601 cggttatctt gttcaaagcg ttgttgctgc tcacgggcat ccgttaattc atggaaacca
      661 ttcgccagct caataccttt ataataaacc tcaaagcgtt cagcgacccg atgatcttcg
      721 gtactgattt gcgccagtga tgcctggctg gctggaaagt ggtacacaaa ggtcggtttt
      781 tctttgccaa tatttggctc tacgccaaag gtaaacagca attgtagcaa cgtgtcgcgg
      841 tcttcttcgg tatcagcaac gttactcaaa tccagtttcg ctgcgacttc ccgcagttgc
      901 gttttgtcgg cagagagcgg gtcaatttcc agataacgca agaaagcttg ttgataagaa
      961 aggctttctg ctgccgggca gtccagcacc tgttgtaaga gatcgtccac ctcgttcatc
     1021 aaccggtaca tatcatagtg cggtcgatac cactcaagca tagtgaactc agggttgtga
     1081 taacgcccca tctcttcatt acggaagctg cggcacagct ggaataccgg cccacaaccg
     1141 gcaaccagca ggcgtttcat atggtattcc gggctggtca ttaaccagag attcatcccc
     1201 tgcgaatgcc cggggccaac gaaacgtgtc tcaaacggga ccaaatgaat atcggttacc
     1261 gtcgcctggc tcatacaagg cgtctccacc tccagcactc cacgatcggc aaagaaacga
     1321 cggatctccg ccataatcgc cgcacgtttt aataagttag gaatggatgc gctcggctgc
     1381 caggatgccg tttcgctcat agttaaatct ccagtttttg acaagggcac gaagtctact
     1441 cgcaacgcga cggcgagaca aattttacgc aggaatcaaa cagcggtggg cagtgactaa
     1501 aaaaagcacg atctgatggt ttagtaatta aattaatcat cttcagtgat aatttagccc
     1561 tcttgcgcac taaaaaaatc gatctcgtca aatttcagac ttatccatca gactatactg
     1621 ttgtacctat aaaggagcag tggaatagcg ttcgcagacc gtaactttca ggcacttacc
     1681 ctgaagtacg gggctgtggg ataaaaacaa tctggaggaa tgtcgtgcaa acctttcaag
     1741 ccgatcttgc cattgtaggc gccggtggcg cgggattacg tgctgcaatt gctgccgcgc
     1801 aggcaaatcc aaatgcaaaa atcgcactaa tctcaaaagt atacccgatg cgtagccata
     1861 ccgttgctgc agaagggggc tccgccgctg tcgcgcagga tcatgacagc ttcgaatatc
     1921 actttcacga tacagtagcg ggtggcgact ggttgtgtga gcaggatgtc gtggattatt
     1981 tcgtccacca ctgcccaacc gaaatgaccc aactggaact gtgggggtgc ccatggagcc
     2041 gtcgcccgga tggtagcgtc aacgtacgtc gcttcggcgg catgaaaatc gagcgcacct
     2101 ggttcgccgc cgataagacc ggcttccata tgctgcacac gctgttccag acctctctgc
     2161 aattcccgca gatccagcgt tttgacgaac atttcgtgct ggatattctg gttgatgatg
     2221 gtcatgttcg cggcctggta gcaatgaaca tgatggaagg cacgctggtg cagatccgtg
     2281 ctaacgcggt cgttatggct actggcggtg cgggtcgcgt ttatcgttac aacaccaacg
     2341 gcggcatcgt taccggtgac ggtatgggta tggcgctaag ccacggcgtt ccgctgcgtg
     2401 acatggaatt cgttcagtat cacccaaccg gtctgccagg ttccggtatc ctgatgaccg
     2461 aaggctgccg tggtgaaggc ggtattctgg tcaacaaaaa tggctaccgt tatctgcaag
     2521 attacggcat gggcccggaa actccgctgg gcgagccgaa aaacaaatat atggaactgg
     2581 gtccacgcga caaagtttct caggccttct ggcacgaatg gcgtaaaggc aacaccatct
     2641 ccacgccgcg tggcgatgtg gtttatctcg acttgcgtca cctcggcgag aaaaaactgc
     2701 atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta cgttggcgtc gatccggtta
     2761 aagaaccgat tccggtacgt ccgaccgcac actacaccat gggcggtatc gaaaccgatc
     2821 agaactgtga aacccgcatt aaaggtctgt tcgccgtggg tgaatgttcc tctgttggtc
     2881 tgcacggtgc aaaccgtctg ggctccaact ccctggcgga actggtggtc ttcggccgtc
     2941 tggccggtga acaagcgaca gagcgtgcag caactgccgg taatggcaac gaagcggcaa
     3001 ttgaagcgca ggcagctggc gttgaacaac gtctgaaaga tctggttaac caggatggcg
     3061 gcgaaaactg ggcgaagatc cgcgacgaaa tgggcctggc aatggaagaa ggttgcggta
     3121 tctaccgtac gccggaactg atgcagaaaa ccatcgacaa gctggcagag ctgcaggaac
     3181 gcttcaagcg cgtgcgcatc accgacacct ccagcgtgtt caacaccgac ctgctctaca
     3241 ccattgaact gggccacggt ctgaacgttg ctgaatgtat ggcgcactcc gcaatggcac
     3301 gtaaagagtc ccgcggcgca caccagcgtc tggacgaagg ttgcaccgag cgtgacgacg
     3361 tcaacttcct caaacacacc ctcgccttcc gcgatgctga tggcacgact cgcctagagt
     3421 acagcgacgt gaagattact acgctgccgc cagctaaacg cgtttacggt ggcgaagcgg
     3481 atgcagccga taaggcggaa gcagccaata agaaggagaa ggcgaatggc tgagatgaaa
     3541 aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg ataccgcacc gcatagcgca
     3601 ttctatgaag tgccttatga cgcaactacc tcattactgg atgcgctggg ctacatcaaa
     3661 gacaacctgg caccggacct gagttaccgc tggtcctgcc gtatggcgat ttgtggttcc
     3721 tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct gcgtgattac
     3781 accgacggta tgaaggttga agcgttagct aacttcccga ttgaacgcga tctggtggtc
     3841 gatatgaccc acttcatcga aagtctggaa gcgatcaaac cgtacatcat cggcaactcc
     3901 cgcaccgcgg atcagggtac taacatccag accccggcgc agatggcaaa gtatcaccag
     3961 ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt tggcctgaac
     4021 ccagagttca tcggtccggc tgccattacg ctggcgcatc gttataacga agatagccgc
     4081 gaccacggta agaaggagcg tatggcgcag ttgaacagcc agaacggcgt atggagctgt
     4141 actttcgtgg gctattgctc cgaagtctgc ccgaaacacg tcgatccggc tgcggccatt
     4201 cagcagggca aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa accacgctaa
     4261 ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt ccacctggtg
     4321 gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa ggcacggcgg ttccggctgt
     4381 gtggttcagc attgaactga ttttcgggct gtttgccctg aaaaatggcc cggaagcctg
     4441 ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg atcattaacc tgatcactct
     4501 ggcggcagct ctgctgcaca ccaaaacctg gtttgaactg gcaccgaaag cggccaatat
     4561 cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc aaaagtctct gggcggtaac
     4621 tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac tggtaaggag cctgagatga
     4681 ttaatccaaa tccaaagcgt tctgacgaac cggtattctg gggcctcttc ggggccggtg
     4741 gtatgtggag cgcaattatt gcgccggtga tgatcctgct ggtgggtatt ctgctgccac
     4801 tggggctgtt tccgggtgat gcgctgagct acgagcgcgt tctggcgttc gcgcagagct
     4861 tcattggtcg cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt ggtttacacc
     4921 gtatgcacca cgcgatgcac gatctgaaaa ttcacgtgcc tgcgggcaaa tgggttttct
     4981 acggtctggc tgctatcctg acagttgtca cgctgattgg tatcgttaca atctaacgta
     5041 tcgccaatgt aaatccggcc cgcctatggc gggccgtttt gtatggaaac cagaccttat
     5101 gttcaaaacg acgctctgcg ccttattaat taccgcctct tgctccacat ttgctgcccc
     5161 tcaacaaatc aacgatattg tgcatcgcac aattaccccg cttatagagc aacaaaagat
     5221 ccccggtatg gcggtggcgg taatttatca gggtaaacct tattacttta cctggggcta
     5281 tgcggacatc gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt taggttcggt
     5341 cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt gctcgagggg aaatcaagtt
     5401 aagcgatccc gcaacaaaat actggcctga acttactgct aaacagtgga atgggatcac
     5461 actattacat cttgcgacct acaccgctgg cggcctgcca ttgcaggtgc cggatgaagt
     5521 gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg cagcctgcat gggctccagg
     5581 aacacaacgt ctgtatgcca actccagtat cggtttgttc ggcgcactgg ctgtgaagcc
     5641 gtctggtttg agttttgagc aggcgatgca aactcgtgtc ttccagccac tcaaactcaa
     5701 ccatacgtgg attaatgtac ctcccccaga agaaaagaat tacgcctggg gatatcgcga
     5761 aggtaaggca gtgcatgttt cgccaggggc gttagatgct gaagcttatg gtgtgaagtc
     5821 gaccattgaa gatatggccc gctgggtgca aagcaattta accccccttg atatcaatga
     5881 gaaaacgctt caacaaggga tacaactggc acaatctcgc tactggcaaa ccggcgatat
     5941 gtatcagggc ctgggctggg aaatgctgga ctggccggtc aatcctgaca gcatcattaa
     6001 cggcagtgac aataaaattg cactggcagc acgcccggta aaagcgatta agcccccaac
     6061 tcctgcagta cgcgcatcat gggtacataa aacgggggcg accggcggat ttggtagcta
     6121 tgtcgcgttt attccagaaa aagagctggg tatcgtgatg ctggctaaca aaaactatcc
     6181 caatccagcg agagtcgccg ccgcctgtca gattctcaac gctctacagt aaaatttcat
     6241 cgggtccgaa ttttcggacc ttttctgcgc ttttccttgc tgtcatctac acttagaaaa
     6301 aaaccagtaa ggaaacattt atgcgcctgc tccctctcgt tgccgcagcg acagctgcat
     6361 ttctggtcgt tgcctgcagt tctcctacgc cgccgcgtgg cgtgacagta gtgaataatt
     6421 tcgacgctaa acgctatctc ggtacctggt atgagattgc ccgttttgat caccgttttg
     6481 aacgtggact ggaaaaagtc accgcaacat acagtctgcg tgatgacggc ggcctgaatg
     6541 tcatcaataa aggctataac cctgacagag aaatgtggca gcagagtgaa gggaaagcgt
     6601 actttaccgg cgcaccaaca cgcgctgcgc tgaaagtgtc attctttggt cctttctatg
     6661 gcggttataa cgtcattgca cttgatcggg aataccgcca tgcgctggtt tgcggcccgg
     6721 accgcgacta cctgtggata ctctcccgca cgccaaccat ttctgacgaa gtgaaacagg
     6781 agatgctggc agtcgcgacc cgggaagggt ttgatgtcag taagtttatt tgggtacagc
     6841 agcccggtag ttagtgagtg ctgagtttca gaccaataat ccccaatacg attaacgcca
     6901 gactcgccag gcgcatcggg ttagcggact caccgagcag cacaatgccg gtgatggccg
     6961 cgccgactgc gccaataccc gtccacacgg cataagccgt ccctaccggt aacgatttca
     7021 tcgcccaggc aagtagcgcc atactgacta tcatcgccgt cacagtaata acacttggcg
     7081 tcaaacgact aaagccgtgg gtatatttca ggccaacggc ccataccact tccagcagac
     7141 cagcaataac taagataatc caggacatat caggctccgg agaattgggg ccgtccccgg
     7201 tgaaaagagg cgtttgcagg tcgtcctgca aagctgatgg ttgaacggat attttgccag
     7261 gtggcagggg aatttcaact attacctttg tgactatcgc aaaaaaacaa acggcacgac
     7321 ataactgtcg taccgttatt gcttattgct gcgctttcgt tgctgcacca gaaatcgcgt
     7381 taccgccatc agaaatgtct tcaccaacgc cacgcgtggt gttgcaggca gttaatactg
     7441 ttgaaagcac cagaacagaa aagatcgctg caattgtctt cttcaccata acgtcttcct
     7501 tttaggtaat gttatttatg tttgcctata gcaaattaat aatagacaac atcgccaaaa
     7561 atgatggaaa tctgatgatt tttcggaaga gaagaaaaat tagctggcag cgcgggagat
     7621 ggagttgccg agatgtttga tgtcttcgcc gaaaccgcga gcggtgttac agcccgtgag
     7681 cagcgtgctg gcaagcaaaa caagaacgat aaggcgtttc atcatctctg acctgaataa
     7741 gtgatggtgc agcctgcagg ccgcaccaca accgcattac ttcacgcgag agacgtattc
     7801 accagagcgg gtatccactt tgatgacttc gccgatttgt acgaacagcg gaactttaac
     7861 cacagcgcca gtagacaggg tagccggttt gccgccagta cctgcggtat cacctttcag
     7921 gcccggatcg gtatcaacga tttccagttc aacgaagttc ggcggagtaa cggagatcgg
     7981 ctgaccattc cacagagtta cgatacactc tgcctgatcc agcagccatt tagcgttgtc
     8041 gccaattgct tttgcatcag cagacagctg ctcgaaagtt tcgttgttca tgaagtgcca
     8101 gaactcaccg tcgttgtaca ggtaagtcag gttcatatcg acaacatcag cgccttcagc
     8161 ggaatcagta gatttgaagg ttttttctac gcgagtaccg gtcagcagac gacgcagttt
     8221 aacgcgagca aatgcctggc ctttacccgg ttttacgaat tcactcgctt caaccgcgta
     8281 aggttcgccg tctaacatga ttttaagacc agcacgaaaa tcgttgctat agtacgttgc
     8341 cataaggccc tctgaaattt gttaattggt agctaagcca caaaatggcg catattgtaa
     8401 ccctaaatac cccatccaga gaagattggt taacgcaact tgccgatgtt gtgaccgatc
     8461 ctggtgaact tctgcgtctt ttgaatatag acgcggacga aaaactgttg gccggacgca
     8521 gcgccaaaaa gctgtttgcc ctgcgtgtgc cccgctcatt tatcgatcgc atggagaaag
     8581 gcaatccgga cgatcctatt ttgcgtcagg tacttacctc gcaagatgag tttgtcgtcg
     8641 cgtccggatt ctccaccgac ccactggaag aacagcacag cgtagtgcct ggtttgttgc
     8701 ataaatacca caaccgggcg cttttgctgg tcaaaggcgg ctgcgcggta aattgccgct
     8761 attgcttccg tcgccacttc ccctatgccg aaaatcaggg caacaagcgt aactggcaga
     8821 ctgcacttga gtatgttgct gcgcatccgg aactggacga gatgattttc tccggcggcg
     8881 atccgctgat ggcgaaagat cacgagctgg actggttgct cacacaactg gaagccatcc
     8941 cgcatatcaa acgtctgcgg attcacagcc gtctgccgat tgtgatcccg gcacgtatca
     9001 ccgaggcgct ggttgaacgc tttgcccgtt ctacgctgca aatcttgctg gtgaatcaca
     9061 tcaatcatgc caatgaggta gatgaaacgt tccgtcaggc gatggctaaa ttgcgccgtg
     9121 tcggtgtcac cctgcttaac cagagcgttc tgttacgtgg tgtgaacgat aacgcacaaa
     9181 cgttggcaaa cctgagtaat gcgttgttcg atgccggcgt gatgccgtat tacctgcatg
     9241 tgctagataa agtacagggc gcggcgcatt ttatggtgag tgatgacgaa gcacggcaga
     9301 ttatgcgtga gttgctgaca ttggtgtcgg gatatctggt gccgaaactg gcgcgagaaa
     9361 ttggcggcga acccagcaaa acgccgctgg atctccagct acgccagcag taactgatta
     9421 aacagggaag ttaaacatta gcctgatgcg ctacgcttat caggcctaca ttgtgcattg
     9481 aaattgaatg gatttgcaat gaactgtagg ccggataagg cgtttacgcc gcatccggca
     9541 agagcaacaa acgcaagtac agcaacctgt cattaaactg ggaaatttaa tctttgcccg
     9601 ttgcataacc tttaattatt catatacatt gctgttgtta ctctttatat agccaacacc
     9661 aataattaac ctttcttttc tgtttatata gagtgagtac ggctatttat gttattttaa
     9721 caactattac taccaagccc atttatctgg cttataaatt aaaattaatt atttaattga
     9781 gaaatggtta gggagaacct acatggccat aagcatcaag ggtgttaata ccggtgttat
     9841 tcgcaagagt aataatttca ttgcactggc gctgaaaatt aaagaacctc gcaataaaga
     9901 atcgttgttc tttatgtctg tcatggaact gcgcgacctg ctgattgcgc tggaaagtcg
     9961 tctgcatcaa aagcataaac tgaatgctgc tgcacgcctt cagtatgagc aagctcgcga
    10021 taaagtcatt aagaaaatgg cagaaaacat cccagaaatt ctggttgatg agcttaaaaa
    10081 tgccgatatt aatcgtcggg ttaacacgct ggaacttacc gataatcaag gcgaaaatct
    10141 taccttcgta ttaacgctac atgacggtag caaatgtgag ttagtcgtca atgagttgca
    10201 gattgaaatg ctggcgcgag caatcattca tgcaattaat aatgcggaaa tgcgtgaact
    10261 ggcattacgt attacctcac tgttagattt cctgccgtta tacgacgttg actgccagga
    10321 caatggcaat ctggaatatg acacctattc ccaaccggaa tggaaacata acctgttcga
    10381 ccattatctg gcggtgcttt accgctttaa agatgaaagt ggcaaagaac aatttagcgg
    10441 cacagtggtg aaaacgcgtg aagcaacgcc gggtaaagaa atagaggcga tcactcgccg
    10501 aatgcttgat ttcagcccac gactgaaaaa actcgcgggt gtgccatgtc aggtctatgt
    10561 tcgcaccgta gcggcgaata acgcccaacc gctaacccag gatcaatgct tacgcgcgct
    10621 gcatcactta cgtgttcaat ccaccagcaa aactgcacca caggcgaaat aatctcatct
    10681 ttgctaatgc gaggctggag ccatccagcc tgaaatgact gataacgtga tcgttggtca
    10741 tgccggatgc gacgtaaacg ccttatccgg cctacaaatt ctcaagcatt caatcagttg
    10801 caatgcctgc tgtaggcctg ataagcgaag cgcatcaggc aataatacgt ttgtcaccca
    10861 gcctctggat cacatcagtt cgggcactta taaacctgac cgataatctg gctatccgtc
    10921 gggacaaaac tggacaacat tccctgcgac gggctgctga tgccataaat cacgttaccg
    10981 cccattgcag ccgcctggtt gcgcagatcg tttgctgcgc cgcgcataga accgccctct
    11041 tctccgtgtt gcccggaaag ccagttgctt tgcttacctg tcgcagtacc aatcagctgg
    11101 cactctgcgc ctggctgctc gtccacaatg cgtacactct gaccggcagc actcaattcg
    11161 ttgctggagc tacaacccgc catcagtagc gcggcaccga caatccctgc taagtatttt
    11221 acgtgcatgt tattccccat aatcaatgag ctggacgcca ctcgcgtcgt ccgtgtctga
    11281 atcttatact agaaagatga ccaaaagaaa aacccccaga catttctgcc cgggggtttg
    11341 tttatttctg cgaggtgcag ggcaattaca tcatgccgcc catgccaccc atgccgccca
    11401 taccgccagc agcgcctaag tcagctgcat catttttcgg caggtcggta accatgcact
    11461 cggtggtgat catcaggcca gccacagaag ccgcgtactg cagagcagaa cgggttactt
    11521 tggttgggtc caggataccc atgtcgatca tgttgccgta ttcttcggtt gctgcgttgt
    11581 aaccgtagtt gccgtcgccg cctttaacgg tgttagcaac aacagacggt tcttcgccgc
    11641 agttcaggac gatctgacgc agcggagctt ccattgcacg cagtgcaact ttgataccca
    11701 cgttctggtc ttcgttctga ccacgcaggt cagccagttt agacgctacg cggatcagcg
    11761 caacaccacc accagcaacc acgccttctt ctaccgcagc acgggtcgcg tgcagggcgt
    11821 cttcaacgcg tgcttttttc tctttcattt caacttcggt agcagcacct actttgataa
    11881 ctgcaacgcc gcctgccagt ttcgctacgc gttcctgcag tttttcacgg tcgtagtcag
    11941 aagttgcttc ttcaatctgc tgacggatct gagcaacacg gccctggatt gcagcttctt
    12001 cacccacgcc atcgatgata gtggtggtgt ctttgttgat cacaacacgt ttagcctgac
    12061 ccaggtcttc cagggttgct ttttccagtt ccataccgat ctcttcagag atcacggtac
    12121 cgccagtcag ggttgcgata tcctgcagca tagctttacg acgatcgccg aagcccggag
    12181 ctttaactgc agcaactttc acgatgccac gcatggtgtt aacaaccaga gttgccagcg
    12241 cttcgccttc tacatcttca gcgatgatca gcagcggttt gcctgctttg gcaacggctt
    12301 ccagaaccgg cagcatttcg cggatgttgg agattttctt gtcagccagc aggatgaacg
    12361 ggctttccag ttctactgcg ccagtttccg gcttgttgat gaagtaagga gacaggtagc
    12421 cacggtcgaa ctgcatacct tcaaccacgt ccagttcgtc ctgcagaccg gtaccgtctt
    12481 caacggtgat aacgccttct ttaccgactt tgtccatcgc ttcagcgatc agtttaccta
    12541 cggtttcgtc ggagttagcg gagatggtac caacctgagc aatcgcttta gagtcagagc
    12601 acggtacgga cagcgctttc agttcttcaa ctgcagcggt aacggctttg tcgataccac
    12661 gtttcaggtc catcgggttc atgcccgcag caacagcttt cagaccctca gtgatgatag
    12721 cctgagccag tacagttgca gtggtggtac cgtcgcctgc agcgtcgttc gctttagagg
    12781 caacttcttt caccatctgc gcacccatgt tttcgaactt gtcttccagt tcgatttcac
    12841 gagcaacgga aacaccatct ttggtgatgg tcggtgcacc gaaagattta tccagaacta
    12901 cgttacggcc tttcggaccg agggtaactt tcactgcatc tgccagtacg tttacgccgc
    12961 gcagcatttt cacacgagcg tcgttaccga attttacgtc tttagctgcc attatcttta
    13021 ttccttaaat tcgtatgttc agtgtcgtgc gaggattacg cttcaacaat tgccagaatg
    13081 tcgctttcgg acatgatcaa cacttcttca ttgtcgatct tctcagattt cacaccgtag
    13141 ccatcgttga aaataacgat gtcgccaact ttcacatcca gcggcttcac ttcgccattt
    13201 tcaaggatac ggccattgcc gacagccagc acttcgccgc gggtggattt agccgctgca
    13261 gagccggtca gaacgatgcc gccagcagat ttagtttcaa cttctttacg cttgacgatc
    13321 acgcgatcat gcaatggacg aatattcatt gataactctc ctttgagaaa gtccgtatct
    13381 gttatgggtg acgccggagc ttacgtggtt tcccggctgg tgaccagaga aatggggatg
    13441 aggattcgcc ccttcaaggg ggaaaaagaa aaaaaattct gatcagcaca aaatcgggtg
    13501 aaaaccctga ttcacctcac atttcatcgc aatttcttca tcgccgtcat aagcgaatct
    13561 gattgtgcta ccatcgaaaa tctacgcagt tgcccaaaat ttgggcgcaa tcggatatca
    13621 acccatgagt ggactcaaac aagaactggg gctggcccag ggcattggcc tgctatcgac
    13681 gtcattatta ggcaccggcg tgtttgccgt tcctgcgtta gctgcacagg tagcaggcaa
    13741 taacagcctg tgggcgtggc ccgttttgat tatcttagtg ttcccgattg cgattgtgtt
    13801 tgcgattctg ggtcgccact atcccagcgc aggcggtgtc gcgcacttcg tcggtatggc
    13861 gtttggttcg cggcttgagc gagtcaccgg ctggttgttt ttatcggtca ttcccgtggg
    13921 tttgcctgcc gcgctacaaa ttgccgccgg gttcggccag gcaatgtttg gctggcatag
    13981 ctggcaactg ttgttggcag aactcggtac gctggcgctg gtgtggtata tcggtactcg
    14041 cggtgccagt tccagtgcta atctacaaac cgttattgcc ggacttatcg tcgcgctgat
    14101 tgtcgctatc tggtgggcgg gcgatatcaa acctgcgaat atccccttcc cggcacctgg
    14161 taatatcgaa cttaccgggt tatttgctgc gttatcagtg atgttctggt gttttgtcgg
    14221 tctggaagca tttgcccatc ttgcctcgga atttaaaaat ccagagcgtg attttcctcg
    14281 cgcattgatg attggtctgc tgctggcagg attagtctac tggggctgta cggtagtcgt
    14341 cttacacttc gacgcctatg gtgaacaaat ggccgcggca gcatcgcttc caaaaattgt
    14401 agtgcaactg ttcggtgtag gagcgttatg gattgcctgc gtaattggct atctgacctg
    14461 ctttgccagt ctcaacattt atatacagag cttcgcccgc ctggtctggt cgcaggcgca
    14521 acataatcct gaccactacc tggcacgcct ctcttctcgc catatcccga ataatgccct
    14581 caatgcggtg ctcggctgct gcgtggtgag cactttggtg attcatgctt tagagatcaa
    14641 tctggacgct cttattattt atgccaatgg catctttatt atgatttatc tgttatgcat
    14701 gctggcaggc tgtaaattat tgcaaggacg ttatcgacta ctggcggtgg ttggcgggct
    14761 gttatgcgtt ctgttactgg caatggtcgg ctggaaaagt ctctatgcgc tgattatgct
    14821 ggcggggtta tggctgtttc tgccaaaacg aaaaacgccg gaaaatggca taaccacata
    14881 atccggcgtt tcgacattaa tcctagcgat cgtctttatg atcaaggcgg tcgcggtcat
    14941 catcctttcg ctggtactca ccatcaaaag tattaccgcc accggtcccg gcgctaaaac
    15001 cgccgcctgg catgcgagaa aagcgcaaat gtggcatcaa cttcactgtc agatgctttt
    15061 gcaccggcgg caataaaagc agaagaccga ggaagtcggt gaaaaagccc ggtaataaaa
    15121 gcagcaaacc ggcaataatc agcgaaacac ttttaatcat ctccgccgca gggttttcac
    15181 ccgccgccat tttttgctgc atcagcacga aattcttaaa gccctggtta cgtaccagtg
    15241 acataccgat aactgacgtg aatataacca gcacgagggt cagcaatacc cccaatacat
    15301 gggcaacctg aataaagatt gaaatctcaa tatagacata aaggaaaatg gcaataaaag
    15361 gtaaccagcg caaaggtttc tcctgtaata gcagccggtt aaacccggct acctgaatgg
    15421 gttgcgaatc gcgttcagct tatattgtgg tcattagcaa aatttcaaga tgtttgcgca
    15481 actatttttg gtagtaatcc caaagcggtg atctatttca caaattaata attaaggggt
    15541 aaaaaccgac acttaaagtg atccagatta cggtagaaat cctcaagcag catatgatct
    15601 cgggtattcg gtcgatgcag gggataatcg tcggtcgaaa aacattcgaa accacatata
    15661 ttctgtgtgt ttaaagcaaa tcattggcag cttgaaaaag aaggttcaca tgtcaaacaa
    15721 cattcgtatc gaagaagatc tgttgggtac cagggaagtt ccagctgatg cctactatgg
    15781 tgttcacact ctgagagcga ttgaaaactt ctatatcagc aacaacaaaa tcagtgatat
    15841 tcctgaattt gttcgcggta tggtaatggt taaaaaagcc gcagctatgg caaacaaaga
    15901 gctgcaaacc attcctaaaa gtgtagcgaa tgccatcatt gccgcatgtg atgaagtcct
    15961 gaacaacgga aaatgcatgg atcagttccc ggtagacgtc taccagggcg gcgcaggtac
    16021 ttccgtaaac atgaacacca acgaagtgct ggccaatatc ggtctggaac tgatgggtca
    16081 ccagaaaggt gaatatcagt acctgaaccc gaacgaccat gttaacaaat gtcagtccac
    16141 taacgacgcc tacccgaccg gtttccgtat cgcagtttac tcttccctga ttaagctggt
    16201 agatgcgatt aaccaactgc gtgaaggctt tgaacgtaaa gctgtcgaat tccaggacat
    16261 cctgaaaatg ggtcgtaccc agctgcagga cgcagtaccg atgaccctcg gtcaggaatt
    16321 ccgcgctttc agcatcctgc tgaaagaaga agtgaaaaac atccaacgta ccgctgaact
    16381 gctgctggaa gttaaccttg gcgcaacagc aatcggtact ggtctgaaca cgccgaaaga
    16441 gtactctccg ctggcagtga aaaaactggc tgaagtcact ggcttcccat gcgtaccggc
    16501 tgaagacctg atcgaagcga cctctgactg cggcgcttat gttatggttc acggcgcgct
    16561 gaaacgcctg gctgtgaaga tgtccaaaat ctgtaacgac ctgcgcttgc tctcttctgg
    16621 tccacgtgcc ggcctgaacg agatcaacct gccggaactg caggcgggct cttccatcat
    16681 gccagctaaa gtaaacccgg ttgttccgga agtggttaac caggtatgct tcaaagtcat
    16741 cggtaacgac accactgtta ccatggcagc agaagcaggt cagctgcagt tgaacgttat
    16801 ggagccggtc attggccagg ctatgttcga atccgttcac attctgacca acgcttgcta
    16861 caacctgctg gaaaaatgca ttaacggcat cactgctaac aaagaagtgt gcgaaggtta
    16921 cgtttacaac tctatcggta tcgttactta cctgaacccg ttcatcggtc accacaacgg
    16981 tgacatcgtg ggtaaaatct gtgccgaaac cggtaagagt gtacgtgaag tcgttctgga
    17041 acgcggtctg ttgactgaag cggaacttga cgatattttc tccgtacaga atctgatgca
    17101 cccggcttac aaagcaaaac gctatactga tgaaagcgaa cagtaatcgt acagggtagt
    17161 acaaataaag aaggcacgtc agatgacgtg ccttttttct tgtgagcagt aacttaaaaa
    17221 taacaatcta atatcaactt gttaaaaaac aaggaaggct aatatgctag ttgtagaact
    17281 catcatagtt ttgctggcga tcttcttggg cgccagattg gggggaatag gtattggttt
    17341 tgcaggcgga ttgggggtgc tggttcttgc cgctattggc gttaaacccg gtaacatccc
    17401 gttcgatgtc atttccatta tcatggcggt tatcgccgct atttctgcca tgcaggttgc
    17461 tggcggtctg gactatctgg ttcatcagac agaaaagctg ctgcgccgta acccgaaata
    17521 catcacaatc ctcgcaccga tcgtgaccta tttcctgaca atctttgctg gtactggcaa
    17581 catctctctg gcgacactgc cagttatcgc tgaagttgcg aaggaacaag gcgttaaacc
    17641 ttgccgtccg ctgtctactg cagtggtatc cgcgcagatt gcgatcaccg catcgccaat
    17701 ctcagcggca gtggtttaca tgtcttccgt gatggaaggt catggcatca gctacctcca
    17761 tctgctctcc gtggtcatcc cgtccaccct gctggcggtt ctggtgatgt ccttcctggt
    17821 cactatgctg ttcaactcca aactctctga cgatccgatt tatcgcaagc gtctggaaga
    17881 gggcctggtt gaactgcgcg gtgaaaagca gattgaaatc aaatccggtg caaaaacgtc
    17941 cgtctggctg ttcctgctgg gcgtagttgg cgtggttatc tatgcaatca tcaacagccc
    18001 aagcatgggt ctggttgaaa aaccgctgat gaacaccacc aacgcaatcc tgatcatcat
    18061 gctcagcgtt gcaactctga ccaccgttat ctgtaaagtc gataccgaca acatcctcaa
    18121 ctccagcacc ttcaaagcag gtatgagcgc ctgtatttgt atcctgggtg ttgcgtggct
    18181 gggcgatact ttcgtttcca acaacatcga ctggatcaaa gataccgctg gtgaagtgat
    18241 tcagggtcat ccgtggctgc tggccgtcat cttcttcttt gcttctgctc tgctgtactc
    18301 tcaggctgca accgcaaaag cactgatgcc gatggctctg gcactgaacg tttctccgct
    18361 gaccgctgtt gcttctttcg ctgcggtgtc tggtctgttc attctgccga cctatccgac
    18421 gctggttgct gcggtacaga tggatgacac gggtacaacc cgtatcggta aattcgtctt
    18481 caaccatccg ttcttcatcc cgggtactct gggtgttgcc ctggccgttt gcttcggctt
    18541 cgtgctgggt agcttcatgc tgtaatgacc aatcgcgggg cgttcacgcc ccgctttctt
    18601 tcccgccgac taacatcctt cccccgtccg ttgtatagtg acctctctct tgcggttcca
    18661 tctgttcttg cgaggtgttt atgcttgatg aaaaaagttc gaataccgcg tctgtcgtgg
    18721 tgctatgtac cgcaccggat gaagcgacag cccaggattt agccgccaaa gtgctggcgg
    18781 aaaaactggc ggcctgcgcg accttgatcc ccggcgctac ctctctctat tactgggaag
    18841 gtaagctgga gcaagaatac gaagtgcaga tgattttaaa aactaccgta tctcaccagc
    18901 aggcactgct ggaatgcctg aagtctcatc atccatatca aaccccggaa cttctggttt
    18961 tacctgttac acacggagac acagattacc tctcatggct caacgcatct ttacgctgat
    19021 cctgctactt tgcagcactt ccgtttttgc cggattattc gacgcgccgg gacgttcaca
    19081 ttttgtcccc gcggatcaag cctttgcttt tgattttcag caaaaccaac atgacctgaa
    19141 tctgacctgg cagatcaaag acggttacta cctctaccgt aaacagatcc gcattacgcc
    19201 ggaacacgcg aaaattgccg acgtgcagct gccgcaaggc gtctggcatg aagatgagtt
    19261 ttacggcaaa agcgagattt accgcgatcg gctgacgctt cccgtaacca tcaaccaggc
    19321 gagtgcggga gcgacgttaa ctgtcaccta ccagggctgt gctgatgccg gtttctgtta
    19381 tccgccagaa accaaaaccg ttccgttaag cgaagtggtc gccaacaacg cagcgtcaca
    19441 gcctgtgtct gttccgcagc aagagcagca caccgcgcaa ttgccctttt ccgcgctctg
    19501 ggcgttgttg atcggtattg gtatcgcctt tacgccatgc gtgctgccaa tgtacccact
    19561 gatttctggc atcgtgctgg gtggtaaaca gcggctctcc actgccagag cattgttgct
    19621 gacctttatt tatgtgcagg ggatggcact gacttacacg gcgctgggtc tggtagttgc
    19681 cgccgcaggg ttacagttcc aggcggcgct acagcaccca tacgtgctca ttggcctcgc
    19741 catcgtcttt accttgctgg cgatgtcaat gtttggcttg tttactctac aactcccctc
    19801 ttcgctgcaa acacatctca cattgatgag caatcgccaa cagggcggct cacctggcgg
    19861 tgtgtttgtt atgggggcga ttgccggact gatctgttca ccatgcacca ccgcaccgct
    19921 tagcgcgatt ctgctgtata tcgcccaaag cgggaacatg tggctgggcg gcggcatgct
    19981 ttatctctat gcgttgggca tgggcctgcc gctgatgcta attaccgtct ttggtaaccg
    20041 cttgctgccg aaaagcggcc cgtggatgga acaagtcaaa accgcgtttg gttttgtgat
    20101 cctcgcactg ccgatcttcc tgctggagcg agtgattggt gatatatggg gattacgctt
    20161 gtggtcggcg ctgggtgtcg cattctttgg ctgggccttt atcaccagcc tacaggccaa
    20221 acgcggctgg atgcgcgtgg tgcaaataat cctgctggca gcggcattgg ttagcgtgcg
    20281 cccacttcag gattgggcat ttggtgcgac gcataccgcg caaactcaga cgcatctcaa
    20341 ctttacacaa atcaaaacgg tagatgagtt aaatcaggcg ctcgttgaag ccaaaggcaa
    20401 accggtgatg ttagatcttt atgccgactg gtgcgtcgcc tgtaaaaagt ttgagaaata
    20461 caccttcagc gacccgcagg tgcaaaaagc gttagcagac acggtcttac ttcaggccaa
    20521 cgtcactgct aacgacgcac aagatgtggc gctgttaaag catcttaatg tccttggact
    20581 accgacaatt ctcttttttg acggacaagg ccaggagcat ccacaagcac gcgtcacggg
    20641 ctttatggat gctgaaacct tcagcgcaca tttgcgcgat cgccaaccgt gaacgacact
    20701 tgcagtggga aagacggagg aaaatagcgt gcaacgtgaa gatgtactgg gagaagccct
    20761 gaaattatta gaattacaag ggattgccaa caccacgctg gagatggttg ctgaacgtgt
    20821 ggattatcca ctggacgagc tacgccgctt ctggccagac aaagaggcaa tcctctacga
    20881 tgcgctgcgt tatcttagcc aacagataga tgtctggcgt cgtcagctga tgcttgacga
    20941 aacgcaaacc gccgaacaaa agctgctggc acgttatcag gcgttatcgg agtgcgttaa
    21001 aaacaaccgc tatccgggct gtctatttat cgctgcctgt acgttttatc ccgaccctgg
    21061 ccaccctatt catcaactgg ccgatcagca aaaaagcgcg gcctacgatt tcacccacga
    21121 actgttaacc acgctggaag ttgacgatcc ggcgatggta gcaaagcaga tggaactggt
    21181 gctggaaggc tgtttaagcc gaatgctggt gaatcgtagc caggcggatg tcgacaccgc
    21241 acatcggctg gcggaagata tcctgcgctt cgcccgctgc cgccagggtg gtgcactgac
    21301 ctgacagaaa cacagaaaag aagcgatttg ccgcaatctt aagcagttga atcgctttta
    21361 ctgaaattag gttgacgaga tgtgcagatt acggtttaat gcgccccgtt gcccggatag
    21421 ctcagtcggt ag
//
LOCUS       DAZPDU010000529        11551 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.guided.137, whole genome shotgun sequence.
ACCESSION   DAZPDU010000529 DAZPDU010000000
VERSION     DAZPDU010000529.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11551
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.guided.137"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            1..652
                     /locus_tag="ACHURS_004901"
                     /pseudo
     CDS             1..652
                     /locus_tag="ACHURS_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            649..1263
                     /gene="prfH"
                     /locus_tag="ACHURS_004902"
     CDS             649..1263
                     /gene="prfH"
                     /locus_tag="ACHURS_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFR1240390.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1320..2777)
                     /gene="pepD"
                     /locus_tag="ACHURS_004903"
     CDS             complement(1320..2777)
                     /gene="pepD"
                     /locus_tag="ACHURS_004903"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFR1240391.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3038..3496
                     /gene="gpt"
                     /locus_tag="ACHURS_004904"
     CDS             3038..3496
                     /gene="gpt"
                     /locus_tag="ACHURS_004904"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFR1240392.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3588..4832
                     /gene="frsA"
                     /locus_tag="ACHURS_004905"
     CDS             3588..4832
                     /gene="frsA"
                     /locus_tag="ACHURS_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFR1240393.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4890..5291
                     /gene="crl"
                     /locus_tag="ACHURS_004906"
     CDS             4890..5291
                     /gene="crl"
                     /locus_tag="ACHURS_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFR1240394.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5330..6385)
                     /gene="phoE"
                     /locus_tag="ACHURS_004907"
     CDS             complement(5330..6385)
                     /gene="phoE"
                     /locus_tag="ACHURS_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFR1240395.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            6673..7776
                     /gene="proB"
                     /locus_tag="ACHURS_004908"
     CDS             6673..7776
                     /gene="proB"
                     /locus_tag="ACHURS_004908"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFR1240396.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7788..9041
                     /gene="proA"
                     /locus_tag="ACHURS_004909"
     CDS             7788..9041
                     /gene="proA"
                     /locus_tag="ACHURS_004909"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFR1240397.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            9156..9231
                     /locus_tag="ACHURS_004910"
     tRNA            9156..9231
                     /locus_tag="ACHURS_004910"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9189..9191,aa:Thr,seq:cgt)
     gene            9837..9995
                     /locus_tag="ACHURS_004911"
     CDS             9837..9995
                     /locus_tag="ACHURS_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240398.1"
                     /translation="MKNIAAIKRNNRKIHARKFLSTPEGKAWLERKQRENEERKLLSE
                     LKWLREDF"
BASE COUNT         2999 a         2686 c         2915 g         2951 t
ORIGIN      
        1 acaccccatt ataaggggta cgaataaagt aacggggcag tctattttac agcggcatat
       61 tgcctcgttt tcgcatcatg gtttgcctga aggcagtgac gacgcgctac ggtatattgc
      121 ggaacatgac gatgcgctgg cgtttgcgcg tattaatcgc cagctgatct ctttacgcat
      181 aatgcaacag gttaaggcca ccggaagtcc ggttctggat gtggcgcata actttgttag
      241 cgcgtgtcaa atcggcgatc aacagggctg gttgcatcgt aaaggtgcca cgccggatga
      301 caacggtctg gtgattattc ccggttcact cggtgattac tcctagcttg tacagcctgt
      361 tgcgaacgaa aaaacgctcc actcgctggc gcatggggct gggcgtaaat gggggcgcac
      421 cgagtgtaaa gggcgtctgg cagcgaaata cacagcgacg cagctctcac gtactgaact
      481 tggcagtcgg gtaatttgtc gcgataaaca actcatcttt gaagaagcgc cacaagccta
      541 taaatcggct gaaagcgtgg tgcaatgtct ggtgcaggct ggtttaatta ttcctgtcgc
      601 gcggctgcgt ccggtgctaa cgctcaaaaa cagtggaggg aaaaaaggat gatcttgcta
      661 caactctcct cagcgcaggg gccggaagaa tgttgtctcg cagtgaaaaa ggcgctggag
      721 cgattaatta aagaagctgc ccgacaagac gtcgcggtaa cggtgttgga aacagaaacg
      781 ggccgctact ctgacacgct gcgttcagct ctggtttctc tggatggcga taacgcatgg
      841 gcattaagcg aaagctggtg cggcactatt cagtggattt gtccgagtcc gtatcggcct
      901 catcatgggc gcaaaaactg gtttctgggc attgggcgtt ttaccgctga tgagcaggaa
      961 caatcggatg caatccgtta tgagacgttg cgttcgtcgg ggccgggcgg tcaacatgtc
     1021 aataaaaccg actcggcggt acgcgccacg catttggcat ccggtattag cgtgaaggtt
     1081 cagtcagagc gtagtcagca tgctaacaag cggctggcac gattgctgat tgcctggaag
     1141 ctggagcagc agcaacagga aaatagcgcg gtgctgaaat cgcagcggcg aatgttccat
     1201 caccagattg aacgtggcaa cccgagacgg acatttacag ggatggcttt tatcgaagga
     1261 taatgaagga tgaaactgcc ggaaggcgat taaacgccat ccggcagcaa atcaaataat
     1321 tacttcgccg gaatttcttt cagcagttca gtcagcagtg tccagtaatg acctacgctt
     1381 tcgatgtgaa cttgctcatc cggagagtgt ggaccggtga tagttggccc gatagaaacc
     1441 atgtccattt ccggatacgg ttttttgaac agaccacatt ccaggcccgc gtggataatc
     1501 tggatgttcg gcgttttgtt gaacagacgc tgataggttt cacgtaccag atgcatcacc
     1561 ggagaattag cgtccggctg ccagccagga tatgcgcctt tcgcttcggt tttcgcgcca
     1621 gccagtttac ccagcgaatc cagcatgctc accacgtagt ctttaccgct gtcgatcaga
     1681 gaacggatca gacagtgaat ttctacgtta ttatcagtca tggtcaccac accgacgttc
     1741 agggaggttt cgaccacacc ttttgccacg tctgagttgc ggatcacacc gttcggggtg
     1801 gcgttcagca gacgaataaa ggtatcgcga gatttcgcaa tcagggcagc tttatcgttc
     1861 gctacagagt ccagcaacaa ggccagattt ttctctttct ctgccagctc gtttttcagg
     1921 atctcctgat aggtattcac cagagatttc aggacgtcga ctttatcagc tgcgacagca
     1981 atggtcgcaa aggcttcacg cgggatggcg ttacgcagtg tgccgccgtt gaaatcgata
     2041 aggcgcagat ccagttcttc tgcatgaccc gctaggaagc gcaccagcag tttgttggca
     2101 ttacccaggc caacgtggat ttccccgccg gagtgacccc ctttcagacc ttttaaggtt
     2161 aacttgaagg tttcaaaacc agctggaacc gcttcacgat ctaaatgcag gttggaggtg
     2221 aagtcgatac cccccgcaca acccatgtag atttcacctt cttcttcgga gtcggtgtta
     2281 atcagaatat cagcctgcaa ccagttgctc tgtaagccga acgcaccgtc cataccggct
     2341 tcttcggtca tggtcagcag cacttccagc gggccgtgaa ccacgttttc gtcagccaga
     2401 accgccagcg cagacgccat accaatgccg ttatctgcac ccagcgtggt gccgcgcgct
     2461 ttaacccatt cgccatcaat ataaggctgg ataggatctt tcgtgaagtc atgcacggtg
     2521 tcgttatttt tctgcggcac catatcgagg tgggcctgta agacgaccga tttacgattt
     2581 tccatacctg cggtagcagg tttacgaatc aggatattac ctacctgatc gcgttcgaca
     2641 tggaaacctt tctcttttgc ccaaccaaca atgtattcag cgagttgctc ttcatgatag
     2701 gacgggtgag gaatagaaca gattttggca aaaatatccc acagcggctg tggagataat
     2761 tgagacagtt cagacacgtt aagtctcctt gtcgatcacc cgcaaaacag tattgcaggt
     2821 cacagggtta gcagaaaatg ttgtcaacac aagacaggct tgcgagatat gtttgagaat
     2881 accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat
     2941 actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc cctcgaacac
     3001 tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat acatcgtcac
     3061 ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc cttctgaaca
     3121 atggaaaggc attattgccg taagccgtgg cggtctggta ccgggtgcgt tactggcgcg
     3181 tgaactgggt attcgtcatg tcgataccgt ttgtatttcc agctacgatc acgacaacca
     3241 gcgcgagctt aaagtgctaa aacgcgcaga aggcgatggc gaaggcttca tcgttattga
     3301 tgacctggtg gataccggtg gtactgcggt tgcgattcgt gaaatgtatc caaaagcgca
     3361 ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg gttgatgact atgttgttga
     3421 tatcccgcaa gatacctgga ttgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc
     3481 aatctccggt cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc tttttaactt
     3541 caggcgggtt acaatagttt ccagtaagta ttctggaggc tgcatccatg acacaggcaa
     3601 acctgagcga aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc
     3661 gccgctttaa tcacggcgca caaccgcctg tgcagtcggc ccttgatggt aaaaccatcc
     3721 ctcactggta tcgcatgatt aaccgtctga tgtggatctg gcgcggcatt gacccacgtg
     3781 aaatcctcga cgtccaggcg cgtattgtga tgagcgatgc cgaacgtacc gacgatgatt
     3841 tatacgatac ggtgattggc taccgtggtg gcaactggat ttatgagtgg gccatccagg
     3901 cgatggtgtg gcaacaaaaa gcctgtgcgg aagaagatcc gcaactcagt ggtcgtcact
     3961 ggctgcatgc ggctacgttg tacaacattg ctgcctatcc tcatctgaaa ggagatgacc
     4021 tggccgagca agcgcaggct ttgtcaaacc gtgcctatga agaagccgcc cagcgtctgc
     4081 cgggcacgat gcggcagatg gagtttaccg tacccggcgg tgcgcccatc accggctttt
     4141 tgcatatgcc gaaaggcgat ggcccgttcc cgacagtatt aatgtgtggt ggtctggatg
     4201 cgatgcagac ggactattac agcctgtatg aacgttattt tgcgccgcgc ggcattgcga
     4261 tgctgactat tgatatgccg tcggtgggct tttcatcaaa atggaagctc acccaggact
     4321 ccagcctgtt gcatcagcac gttttaaagg ctctgcctaa cgtaccgtgg gtggatcaca
     4381 ctcgcgtcgc ggcctttggt ttccgtttcg gcgctaacgt tgccgtgcgt ctggcatatc
     4441 ttgaatcgcc gcgtctgaaa gcggttgcct gtcttggtcc ggtagttcat accctgttga
     4501 gtgattttaa gtgccagcaa caggtgccgg aaatgtatct tgacgttctg gcgagtcgtt
     4561 tggggatgca tgatgcttcc gacgaagcgt tacgcgtgga gctgaatcgc tattcattaa
     4621 aagtgcaagg attgctgggg cgtcgctgcc caacgccaat gttatcaggc tactggaaga
     4681 acgatccgtt cagcccggaa gaggactcac gcttaatcac ctcatcatct gctgacggta
     4741 aattattaga gatcccattt aacccggtgt atcggaattt tgacaaaggt cttcaggaaa
     4801 tcaccggctg gatcgaaaaa cgcttgtgtt aaaaatttgc taaaatttgc caatttggta
     4861 aaacagttgc atcacaacag gagattgcaa tgacgttacc gagtggacac ccgaagagca
     4921 gattgatcaa aaaatttacc gcactaggcc cgtatattcg tgaaggtaag tgcgaagata
     4981 atcgattctt tttcgattgt ctggctgtat gcgtcaacgt gaaaccggca ccggaagtgc
     5041 gtgagttctg gggctggtgg atggagcttg aagcgcagga atcccgtttt acctacagtt
     5101 accagtttgg tctgttcgat aaagcaggcg actggaagag tgttccggta aaagacactg
     5161 aagtggttga acgactggag cacaccctac gtgagtttca cgagaagctg cgtgaactgc
     5221 tgacgacgct gaatctgaag ctggaaccgg cggatgattt tcgtgatgaa ccggtgaagt
     5281 taacggcgtg agtgaaatgt gccggatgta tcacatccgg caatatccat taaaactgat
     5341 aggtcatgcc aaccgcgaca atatcatcat tattaatatt caatttgtta tcgctatcca
     5401 gttggttgat tttataatca acaaacgctg acatattttt gttgaaataa tacgtagcac
     5461 cgacgtcgat ataattgacc agatcttcat caccgatacc ttcaatatct ttccctttcg
     5521 ataagacata acccagcgat ggacgcagac caaagtcaaa ctggtattga gcgaccgctt
     5581 caaagttctg tgtcttattg gcaaagccgc cagttattgg cgtcattttg cgtgtttcag
     5641 aatagaaagt tgccagataa atattattgg catcgtattt cagacctgtt gcccatgctt
     5701 ctgcacgctt gcctgtgcca cggctttgca ggttctgctc gttggtgcga tctgagttgg
     5761 tataggcccc actaatggcg aaatcgctgc cgccaaagtc atatgtcaat gacgtgccga
     5821 agccatcgcc gttttgcttt ttaacgtcgc ggttttcgtt tttcccttga tattgcaggg
     5881 ttaagttcag gccatcgata acgccgaaga agtcggtgtt ccgatacgtc gccagaccgc
     5941 tggcgcgttt ggtcataaag ttgtcggtct gcgccgagga atcgccacca aattccggga
     6001 acatatcggt ccaggcttcc acgtcataca acgcgcccag gttacgacca taatcgaaag
     6061 aacccaaatc tttatatttc aacccggcaa aagcgagacg cgttttctgc tgtgcagtat
     6121 cactctccgc tttatttccg gcaaactccg cttcccaccg accataacca gtcagttgat
     6181 cgttaatttg tgtttcacct ttaaaaccaa aacggatata actctggtcg ccatctttac
     6241 tggcgttatc actcatataa tgcatggctt taactttgcc atagacatcc agtttattac
     6301 cgtctttatt atatatttct gcagcctgta cagatgcaga tgccacaatg cccatcacca
     6361 ctaatgccag agtgctcttt ttcattttca ttcctgattt taattaacgc gcgaatattc
     6421 agcgggagag ccccgttgaa aacaggaaag tttttaacct gagattgtta aagatatatt
     6481 acagattaat aatattctta aaatgtggta atttattaaa tctgtaataa aagcgtaaac
     6541 aactgccgct aggcttgctg atcccgcgca acaaaacgcc atgctttgct cgcagatggt
     6601 tggcaaccga cgacagtcct gctaaaacgt tcgtttgata tcatttttcc taaaattgaa
     6661 tggcagagaa tcatgagtga cagccagacg ctggtggtaa aactcggcac cagtgtgcta
     6721 acaggcggat cgcgccgtct gaaccgtgcc catattgttg aacttgttcg ccagtgcgcg
     6781 cagttacatg ccgccgggca tcggattgtt attgtgacgt cgggcgcgat cgccgccgga
     6841 cgtgagcacc tgggttaccc ggaactgcca gcgactatcg cctcgaaaca actgctggca
     6901 gcggtagggc agagtcgact gattcaactg tgggaacagc tgttttcgat ttatggcatt
     6961 cacgtcgggc aaatgctgct gacccgtgct gatatggaag accgtgaacg cttcctgaac
     7021 gcccgcgaca ccctgcgtgc gttgctcgat aacaatatcg ttccggtaat caatgagaac
     7081 gatgctgtcg ctacggcaga gattaaagtc ggcgataacg ataacctttc tgcgctggcg
     7141 gcgattctgg cgggtgccga taaactgttg ctgctgaccg atcaaaaagg tttgtatacc
     7201 gctgacccgc gcagcaatcc gcaggcagaa ctgattaaag atgtttacgg cattgatgac
     7261 gcactgcgcg cgattgccgg tgacagcgtt tcaggcctcg gaactggcgg catgagtacc
     7321 aaattgcagg ccgctgacgt ggcttgccgt gcgggtatcg acaccattat tgccgcgggc
     7381 agcaagccgg gcgttattgg tgatgtgatg gaaggcattt ccgtcggtac gctgttccat
     7441 gcccaggcga ctccgcttga aaaccgtaaa cgctggattt tcggtgcgcc gccggcgggt
     7501 gaaatcacgg tagatgaagg ggcaactgcc gccattctgg aacgcggcag ctccctgttg
     7561 ccgaaaggca ttaaaagcgt gactggcaat ttctcgcgtg gtgaagtcat ccgcatttgc
     7621 aacctcgaag gccgcgatat cgcccacggc gtcagtcgtt acaacagcga tgcattacgc
     7681 cgtattgccg gacaccactc gcaagaaatt gatgcaatac tgggatatga atacggcccg
     7741 gttgccgttc accgtgatga catgattacc cgttaaggag ctggccgatg ctggaacaaa
     7801 tgggcattgc tgcgaagcaa gcctcgtata aattagcgca actctccagc cgcgaaaaaa
     7861 atcgcgtgct ggaaaaaatc gccgatgaac tggaagcaca aagcgaaatc atcctcaacg
     7921 ctaacgccca ggatgttgct gacgcgcgag ccaatggcct tagcgaagcg atgcttgacc
     7981 gtctggcact gacgcccgca cggctgaaag gcattgccga cgatgtacgt caggtgtgca
     8041 acctcgccga tccggtgggg caggtaatcg atggcggcgt actggacagc ggcctgcgtc
     8101 ttgagcgtcg tcgcgtaccg ctgggggtta ttggcgtgat ttatgaagcg cgcccgaacg
     8161 tgacggttga tgtcgcttcg ctgtgcctga aaaccggtaa tgcggtgatc ctgcgcggtg
     8221 gcaaagaaac gtgtcgcact aacgctgcaa cggtggcggt gattcaggac gccctgaaat
     8281 cctgcggctt accggcgggt gccgtgcagg cgattgataa tcctgaccgt gcgctggtca
     8341 gtgaaatgct gcgtatggat aaatacatcg acatgctgat cccgcgtggt ggcgctggtt
     8401 tgcataaact gtgccgtgaa cagtcgacaa tcccggtgat cacaggtggt ataggcgtat
     8461 gccatattta cgttgatgaa agtgtagaga tcgctgaagc attaaaagtg atcgtcaacg
     8521 cgaaaactca gcgtccgagc acatgtaata cggttgaaac gttgctggtg aataaaaaca
     8581 tcgccgatag cttcctgccc gcattaagca aacaaatggc ggaaagcggc gtgacattac
     8641 acgcagatgc agctgcactg gcgcagttgc aggcaggccc tgcgaaggtg gttgctgtta
     8701 aagccgaaga gtatgacgat gagtttctgt cattagattt gaacgtcaaa atcgtcagcg
     8761 atcttgacga tgccatcgcc catattcgtg aacacggcac acaacactcc gatgcgatcc
     8821 tgacccgcga tatgcgcaac gcccagcgtt ttgttaacga agtggattcg tccgctgttt
     8881 acgttaacgc ctctacgcgt tttaccgacg gcggccagtt tggtctgggt gcggaagtgg
     8941 cggtaagcac acaaaaactc cacgcgcgtg gcccaatggg gctggaagca ctgaccactt
     9001 acaagtggat cggcattggt gattacacca ttcgtgcgta aataaaaccg ggtgatgcaa
     9061 aagtagccat ttgattcaca aggccattga cgcatcgccc ggttagtttt aaccttgtcc
     9121 accgtgattc acgttcgtga acatgtcctt tcagggccga tatagctcag ttggtagagc
     9181 agcgcattcg taatgcgaag gtcgtaggtt cgactcctat tatcggcacc attaagaaaa
     9241 ttaataactt agcttaactt ctctaagaaa ttgtatcttc tgttatcact tacaaacgcc
     9301 tatacgttgt tatgggcttt ggtagtgtaa cacgtatggg cgagtagttc ccaggctcaa
     9361 cctcgtaaca agttcctttg aagctggaag acgtgccacc gtctctaaac cttctaccaa
     9421 aactcttagc aagatgttcc acacctggcg cggtgtccaa tgatgctaca cgtcgtgaga
     9481 cgttgcagag gataaacagg ctaaatgatg ggaagagttt taagtgcaat agtggattaa
     9541 agcccacctc ttcaaaacct aacttgatcg ggaaagaatc aagctctagc acggtttcgt
     9601 ccaccggagt acaacgggtg cctgataaga gaagaactgc taagacttca tagcttttgt
     9661 gaaaaagccc tttttagtgg atacgcctac ggcgcttatt ttttggtata accatcaaag
     9721 gcgctttatg gctcctattg tcttgaaaat gtatctttac ttgtattgtg ttttatgcgt
     9781 agtctccctg tgtagtatgt aacttgttaa ttttcatatg aatacagagg cacacgatga
     9841 aaaacattgc agcaatcaaa cgtaataacc gcaagattca cgctcgtaag ttcctgtcta
     9901 cgccagaagg aaaagcctgg ctagagcgta aacagagaga gaacgaagaa agaaaactcc
     9961 ttagtgagtt gaaatggctt agagaggatt tctgaaaatc tccagaacgc aacaggacgc
    10021 attctaacgc tgttttcatt actcggtata gggataggtg atgtgtgttt aatgcgtctg
    10081 tgcgcttgtt ttgtgcctta ttttgcattt tcataatgcg gaagagaaca cggaaaacct
    10141 taaaattaat tattggggta tttaaggcta ttttcaggtg atttaagggt attaaacttg
    10201 attaaaatat aaacagataa ttatgtatct tattgattta taaggttatt tttaagtaaa
    10261 ggcaaataaa aaataaatag ccataaatag ctataaatac atcttaatac ctatatccct
    10321 tcccttatgg ctagattgcg agaggtgctt aacgcgattt tcagcgcccc ccgatctgga
    10381 aaatccagaa tccccaaata tcagcaagga attttcttgc gtaatcaaca tcgtttttac
    10441 ctattaaatc attacacaga aagcctttct gtaatgtctc tcactaaact ttcactaaac
    10501 acgagaatga tcggttttat gtcggatcag atgctagaaa cagcaccacg tctcacaagg
    10561 gctgtaagcg atgaaaccag tgtttatgcg ggtgcaggtc aaaatatagg ccaaaatcca
    10621 cttaacataa taaatgttat atgtgctaag gaacacatat aacgccttga attgatgtat
    10681 caggggaaaa gtgatggatt ttttacgtag gagcacgaaa gggcttctat tgacttttgt
    10741 atgctatgaa atataattaa agttttttgt ttttattgtg tgatgtggtg ggttacacag
    10801 gggaatagat taaatggtgt tctgtttata caggtttatt tgttagatca acaatttggc
    10861 atttctaaac agatgtttga acgttgccaa atgtcttgac aacacaagaa aagggcttgc
    10921 tatgccaggc attacacgaa ataaggctat aaaaacaaaa taaattttaa aatttgcaca
    10981 aacgagaaac tcgttcagaa acaagcagaa caatttttag ccaacaattt ctgatggtgc
    11041 tctgtaagcc gcattacaca aggctttgcg aaagtgatgt aaaagtgaag aaagtaagaa
    11101 ttgaactttt aagctacttt tcattggatt ttttagtagg gaattacaca gggaatagag
    11161 agcattattc tatgatttac acagggaata tagggggatt atggcacaca catagaaaga
    11221 cattacacag agtgctatgt gacaaatgga tgtaacgaga ggttacattc atttatctga
    11281 aatgctctct gtaagaaagt tggtagtaac gatatgttac atagactaat tattgaatgt
    11341 aacgatatta cacatcggta gtaacgatac gttacatcgg aagatgattg gatgtaacgg
    11401 tagctcacag tgaactaggg gtaggtcata gtgaactacc cccacagaat tctgttaccc
    11461 ctatcaagtc tgatacccct atcaaaactg atacccgata agtataaata acacctaaat
    11521 agctgcgcgg aatagtagat cactttgagg g
//
LOCUS       DAZPDU010000530         6065 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.guided.229, whole genome shotgun sequence.
ACCESSION   DAZPDU010000530 DAZPDU010000000
VERSION     DAZPDU010000530.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6065
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.guided.229"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            <1..816
                     /gene="sul2"
                     /locus_tag="ACHURS_004912"
     CDS             <1..816
                     /gene="sul2"
                     /locus_tag="ACHURS_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HFR1240399.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            903..1205
                     /locus_tag="ACHURS_004913"
     CDS             903..1205
                     /locus_tag="ACHURS_004913"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240400.1"
                     /translation="MVRKYFGTDGIRGKANEGAMTAETALRVGMAAGRVFRRGDHRHR
                     VVIGKDTRLSGYMLEPALTAGFTSMGMDVFLFGPLPTTYRKNKRPFHPSPTALDRS"
     gene            complement(1381..2874)
                     /locus_tag="ACHURS_004914"
     CDS             complement(1381..2874)
                     /locus_tag="ACHURS_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISVsa3 family transposase"
                     /protein_id="HFR1240401.1"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMA
                     EQGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARR
                     MAESAALLVDEVLPEQPMRQWVLSFPFQLRFLFASRPEIMGWVLGIVYRVIATHLVKK
                     AGHTHQVAKTGAVTLIQRFGSALNLNVHFHMLFLDGVYVEQSHGSARFRWVKAPTSPE
                     LTQLTHTIAHRVGRYLERQGLLERDVENSYLASDAVDDDPMTPLLGHSITYRIAVGSQ
                     AGRKVFTLQTLPTSGDPFGDGIGKVAGSSLHAGVAARADERKKLERLCRYISRPAVSE
                     KRLSLTRGGNVRYQLKTPYRDGTTHVIFEPLDFIARLAALVPKPRVNLTRFHGVFAPN
                     SRHRALVTPAKRGRGNKVRVADEPATPAQRRASMTWAQRLKRVFNIDIETCSGCGGAM
                     KVIACIEDPIVIKQILDHLKHKAETSGTRALPESRAPPAELLLGLFD"
     gene            complement(2918..3307)
                     /locus_tag="ACHURS_004915"
     CDS             complement(2918..3307)
                     /locus_tag="ACHURS_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240402.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            3525..3965
                     /locus_tag="ACHURS_004916"
     CDS             3525..3965
                     /locus_tag="ACHURS_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HFR1240403.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            complement(4688..4837)
                     /locus_tag="ACHURS_004917"
     CDS             complement(4688..4837)
                     /locus_tag="ACHURS_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240404.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            5408..5566
                     /locus_tag="ACHURS_004918"
     CDS             5408..5566
                     /locus_tag="ACHURS_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR1240405.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDR
                     LRGLEVEA"
BASE COUNT         1442 a         1672 c         1586 g         1365 t
ORIGIN      
        1 atgaataaat cgctcatcat tttcggcatc gtcaacataa cctcggacag tttctccgat
       61 ggaggccggt atctggcgcc agacgcagcc attgcgcagg cgcgtaagct gatggccgag
      121 ggggcagatg tgatcgacct cggtccggca tccagcaatc ccgacgccgc gcctgtttcg
      181 tccgacacag aaatcgcgcg tatcgcgccg gtgctggacg cgctcaaggc agatggcatt
      241 cccgtctcgc tcgacagtta tcaacccgcg acgcaagcct atgccttgtc gcgtggtgtg
      301 gcctatctca atgatattcg cggttttcca gacgctgcgt tctatccgca attggcgaaa
      361 tcatctgcca aactcgtcgt tatgcattcg gtgcaagacg ggcaggcaga tcggcgcgag
      421 gcacccgctg gcgacatcat ggatcacatt gcggcgttct ttgacgcgcg catcgcggcg
      481 ctgacgggtg ccggtatcaa acgcaaccgc cttgtccttg atcccggcat ggggtttttt
      541 ctgggggctg ctcccgaaac ctcgctctcg gtgctggcgc ggttcgatga attgcggctg
      601 cgcttcgatt tgccggtgct tctgtctgtt tcgcgcaaat cctttctgcg cgcgctcaca
      661 ggccgtggtc cgggggatgt cggggccgcg acactcgctg cagagcttgc cgccgccgca
      721 ggtggagctg acttcatccg cacacacgag ccgcgcccct tgcgcgacgg gctggcggta
      781 ttggcggcgc tgaaagaaac cgcaagaatt cgttaactgc acattcggga tatttctcta
      841 tattcgcggt tcagcaggca tgtccccttt gagggcgacc cgacgacagg ataatcgacc
      901 ttatggtgcg caaatatttc ggcacagacg gtattcgtgg caaagccaac gaaggcgcga
      961 tgacggcgga aaccgccttg cgcgtcggca tggcggctgg ccgtgtcttt cgtcgcggtg
     1021 accaccgcca tcgtgtcgtg atcggcaagg atacgcgcct gtcgggctat atgcttgaac
     1081 ccgcgctcac agccggtttc acctcgatgg gcatggacgt attccttttt ggcccgctgc
     1141 cgacaacgta taggaagaat aaacgccctt ttcacccaag tccaacagct ttggaccgca
     1201 gttgactctt tcgacacccc tgcgatgcaa cccaatccgg ctgacgggga gccagcaacg
     1261 ctgaaaattt accctcctct ttcccactag cggctccttt tccgacaacc agcacggcgg
     1321 atccctgccg cggcgctgtg aacgcagcat tttgattggt atcgttggcc ttcaggctcg
     1381 tcagtcaaac agacccagga gcagctcagc cggtggcgcc cggctttcgg gtaacgccct
     1441 ggtcccgctg gtttcggctt tgtgcttcag gtgatcaagg atctgcttga tcactatagg
     1501 gtcttcaatg caggcgatga ctttcatggc gccgccgcag ccgctgcagg tctcgatgtc
     1561 gatattgaaa acacgcttga gccgttgcgc ccatgtcatc gacgctcgcc gttgtgctgg
     1621 tgttgccggt tcatcagcca ccctgacctt gttgcccctg ccccgttttg ccggcgtgac
     1681 caacgcccgg tgccgactgt tgggtgcgaa caccccgtgg aagcgggtta ggttgactct
     1741 gggcttcggt accagggcgg ccagccttgc aatgaaatcc aatggttcga aaatgacgtg
     1801 cgtggtgccg tcccggtacg gcgtcttgag ctggtagcgc acgttgccgc ctcgtgttaa
     1861 cgacagccgc ttctcggata ccgccgggcg gctgatgtac cggcacagcc gttcgagctt
     1921 cttgcgttca tcggccctgg ccgccacgcc ggcgtgcagg ctggacccgg ctaccttgcc
     1981 aatcccgtca ccgaacggat caccactggt cggcagagtt tgcaaagtga acacctttcg
     2041 ccccgcctgt gaaccgacag cgatacggta agtgatcgag tgccccagca ggggtgtcat
     2101 cgggtcgtca tccaccgcat ccgaggccag atagctgttt tcgacatccc gttccagcag
     2161 gccttgccgt tccagatagc gacccacccg gtgggcgatg gtgtgcgtca gctgggtgag
     2221 ctctgggctg gtcggcgcct tgacccagcg gaaacgcgct gagccgtggg attgctcgac
     2281 atacacaccg tcgagaaaca gcatgtggaa gtgaacattc agattgagcg ccgatccaaa
     2341 acgctggatc agggtgaccg cgcccgtctt ggccacttgg tgggtatggc ccgctttctt
     2401 gaccaggtgc gtggcaatga cgcggtaaac gatgcccagc acccacccca tgatctcggg
     2461 ccggctggca aacaggaaac gcagctgaaa cgggaagctc aacacccact gacgcatggg
     2521 ttgttcaggc agtacttcat caaccagcaa ggcggcactt tcggccatcc gccgcgcccc
     2581 acagctcggg cagaaaccgc gacgcttaca gctgaaagcg accaggtgct cggcgtggca
     2641 agactcgcag cgaacccgta gaaagccatg ctccagccgc ccgcattgga gaaattcttc
     2701 aaattcccgt tgcacatagc ccggcaattc ctttccctgc tctgccataa gcgcagcgaa
     2761 tgccgggtaa tactcgtcaa cgatctgata gagaagggtt tgctcgggtc ggtggctctg
     2821 gtaacgacca gtatcccgat cccggctggc cgtcctggcc gccacatgag gcatgttccg
     2881 cgtccttgca atactgtgtt tacatacagt ctatcgctta gcggaaagtt cttttaccct
     2941 cagccgaaat gcctgccgtt gctagacatt gccagccagt gcccgtcact cccactctta
     3001 cattgttcgc agtatctagg tacatgttat tgcgtgcacc ttcatgatgt ataatgttat
     3061 taatgacagc agaaagatcg acagaattaa tattcttcgt acctgggaca gttatcgggt
     3121 gtcggccagt gatgagacca aaggatgcag tataagatga accactacta ttatcatggc
     3181 caccattacc accaccgtta aaactacctc ccgaacttcc agagccgcta tgaccatcat
     3241 tgtaggggtc atttggtccc atgtgaccaa aaccaccgac agtaccgcta tttaactctg
     3301 tactcataaa attatcctct ttgactttta aaaacagcaa aaactgtatt tataacaatg
     3361 tgtttatata cagtattcga aatactacgc ccgtcaaaac cacaccgtca acaggttttt
     3421 tgtgtatttt tcacgcctca cgggcagcct cagacctttt gtgttaaaac agtaatatgt
     3481 tctgctgtta caatacggta cacacaggag acactgtcac ttttatggca tggcacacag
     3541 ttcaagagac actgaaatta acaggaaagt cgcgctcgca attatatcgt gacatggcta
     3601 aaggccttgt cagctatcgc acaggaaaaa atgatcgccg ggagttcgag accagtgaac
     3661 tgattcgggc ctatggcgag ctgcgcactt ctgagacacc aaaaaggcac aaggagggac
     3721 actccaaatc tgtcgttagc caggcggaaa atgctcagct agacgtcatt cagcagaaac
     3781 tggacgctct gcagcagact gttaccctaa tgcttgaaga caagatggcg cgggaagcag
     3841 aaaaccgcct gcgaaatgag gagagggaac aactacaggc cgaaattagc cgtctggctg
     3901 acgcgctggc acaggaaaaa aagcggggat tctggtcgcg gctgtttcgc tccggttcag
     3961 agtaacagtg caggtgactc tgatttacca taacatctgt tatcagcacc gcctgagagg
     4021 ccctcagggc gtttctgacg ggaaaccggt aaaacaggca acaaaccacc cgaaaacccg
     4081 ccagaaacgc gcaggctctg tttttacggc atgcatgagt atgcgcacaa gtgcatagcg
     4141 cggaaattca caccattcag ccggggtaag tcctgtttcc ggcgttcttc ccatccgaaa
     4201 acggtgaacg gggacgcaat ttattgcgtg gccgtgaagc gttgccggcg cagccggaaa
     4261 acccgctgtc agcacgttca ggaagggggt gatgtacaaa tggcacagag gatgaaagtg
     4321 acgtatgagc tgaaggaatg atgtaaaagt gatgtatgaa ctgaaggagc gatgtaattc
     4381 tgtagtcagg aagaataacc cgttcatgca ggcatgaacg atacagtgag gaacgctttt
     4441 tttgtcacga tctcccctta acctgcgtaa atcacccgcc atggttgatt atttttcaac
     4501 ctcaaaatgg tcatacacct aacgcagccg cgattttcat cgcaactgca tgaattaaaa
     4561 gaaaaaaaag gaatatttcg ttttcacgag taatacccgg gtattaaaaa tccactgtaa
     4621 ttacatgaaa ataaagaaat aaaaggtaat accggagtat taccacgtac gcgggcaagc
     4681 gaaaaagcta taacgcttcc cggccgacag gcgacccaaa atccacaggg gcatgcaggt
     4741 tctcttcagt aacctgcgcc attaaatcaa aaaggtcata ttcgccggga agctgcgtat
     4801 cagtcgaatc cagttgttca tcatattcag atggcatggg gtgatatcca gtagctgaga
     4861 ggttgttagc gggtttcggg gcgcagccct gaaccagtca tgtagcgcta gcggagtgta
     4921 tactggctta ctatgttgcc acgattgcga aaatcagtga agtgcttcat gtggcaggag
     4981 aaaaaaggca gcaccggcgc gtcagcagaa tatgcgatac aggagatatt ccgcttcctc
     5041 gctcactgac tcgctacgct cggtcgttcg gctgcggcga gcggtaccgg cttacggacg
     5101 gggacggaaa tttcctggaa gataccagga agacacttag caaagaagag ataaggccac
     5161 ggcgaagccg tttttccata ggctccgccc ccctgacaag catcgcaaaa tctgacgctc
     5221 aaatcagtgg tggcgaaacc cgacaggact ataaagatcc caggcgtttc cccctggtag
     5281 ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg
     5341 gccgcggttt ctcattccac gcctgacact cagttccgtg aaggcagttc gctccaagct
     5401 ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc gccttgtccg gtaactatca
     5461 acttgagtcc aacccggaaa gacacgacaa aacgccactg gcagcagcca ttagtaacag
     5521 gagtgcacga gaagatagat taagaggtct tgaagtagag gcctgactgc ggctacactg
     5581 aaagaacagt tttggtaatg gcggtctcgt gaaccctgtt accgggttaa gcagttcccc
     5641 aactgactta accttcgaac aaaccgcctc cccaggcggt tttttcgttt acagagcaag
     5701 agattacgat tcattgaaaa ggatctcaag aagatccttt gtagattact atcaattaat
     5761 cttccgaaca tgacaccagt cactgttgat tacggttaaa ccgctccgta acaggtttcc
     5821 ctcgtattgg tgacttgcag caccaaagaa gcaaaaaaga taaatatatc tacttattga
     5881 gagatgatat atttatttcc aaaatgctac agatacatca tcatgaaacg gatcaccgca
     5941 aacaggttac tcgcctgggg attccctttc gacccgagca tccgtatgag actcatgctc
     6001 gattattatt attatagaag cccccatgaa taaatcgctc atcattttcg gcatcgtcaa
     6061 cataa
//
LOCUS       DAZPDU010000531         5514 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2305874-1R
            SAMN43277764-rid23485963.guided.242, whole genome shotgun sequence.
ACCESSION   DAZPDU010000531 DAZPDU010000000
VERSION     DAZPDU010000531.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277764
            Sequence Read Archive: SRR30464087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396438.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396438.1
            Genome Coverage       :: 534x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,923
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 120 of 271
            Pseudo Genes (incomplete)         :: 94 of 271
            Pseudo Genes (internal stop)      :: 89 of 271
            Pseudo Genes (multiple problems)  :: 31 of 271
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5514
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277764-rid23485963.guided.242"
                     /strain="M2305874-1R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-07-25"
                     /collected_by="FSS"
     gene            complement(<1..172)
                     /locus_tag="ACHURS_004919"
     CDS             complement(<1..172)
                     /locus_tag="ACHURS_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFR1240406.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGSVRPVVTESVTKIMACGTT
                     LMGYTQWCCSSPD"
     gene            complement(281..3286)
                     /locus_tag="ACHURS_004920"
     CDS             complement(281..3286)
                     /locus_tag="ACHURS_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /protein_id="HFR1240407.1"
                     /translation="MPVDFLTTEQTESYGRFTGEPDELQLARYFHLDEADKEFIGKSR
                     GDHNRLGIALQIGCVRFLGTFLTDMNHIPSGVRHFTARQLGIRDITVLAEYGQRENTR
                     REHAALIRQHYQYREFAWPWTFRLTRLLYTRSWISNERPGLLFDLATGWLMQHRIILP
                     GATTLTRLISEVREKATLRLWNKLALIPSAEQRSQLEMLLGPTDCSRLSLLESLKKGP
                     VTISGPAFNEAIERWKTLNDFGLHAENLSTLPAVRLKNLARYAGMTSVFNIARMSPQK
                     RMAVLVAFVLAWETLALDDALDVLDAMLAVIIRDARKIGQKKRLRSLKDLDKSALALA
                     SACSYLLKEETPDESIRAEVFSYIPRQKLAEIITLVREIARPSDDNFHEEMVEQYGRV
                     RRFLPHLLNTVKFSSAPAGVTTLNACDYLSREFSSRRQFFDDAPTEIISRSWKRLVIN
                     KEKHITRRGYTLCFLSKLQDSLRRRDVYVTGSNRWGDPRARLLQGADWQANRIKVYRS
                     LGHPTDPQEAIKSLGHQLDSRYRQVAARLCENEAVELDVSGPKPRLTISPLASLDEPD
                     SLKRLSKMISDLLPPVDLTELLLEINAHTGFADEFFHASEASARVDDLPVSISAVLMA
                     EACNIGLEPLIRSNVPALTRHRLNWTKANYLRAETITSANARLVDFQATLPLAQIWGG
                     GEVASADGMRFVTPVRTINAGPNRKYFGNNRGITWYNFVSDQYSGFHGIVIPGTLRDS
                     IFVLEGLLEQETGLNPTEIMTDTAGASELVFGLFWLLGYQFSPRLADAGASVFWRMDH
                     DADYGVLNDIARGQSDPRKIVLQWDEMIRTAGSLKLGKVQVSVLVRSLLKSERPSGLT
                     QAIIEVGRINKTLYLLNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYT
                     DGQEDQLGTLGLVTNAVVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINML
                     GHYSFTLAELVTKGHLRPLKEASEAENVA"
     gene            3450..4007
                     /locus_tag="ACHURS_004921"
     CDS             3450..4007
                     /locus_tag="ACHURS_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005687610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR1240408.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTD
                     REGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGD
                     MGQMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTG
                     ATEIAHQLSIARSTVYKILEDERAS"
     gene            4190..5050
                     /locus_tag="ACHURS_004922"
     CDS             4190..5050
                     /locus_tag="ACHURS_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="HFR1240409.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            complement(5145..>5514)
                     /locus_tag="ACHURS_004923"
     CDS             complement(5145..>5514)
                     /locus_tag="ACHURS_004923"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1240410.1"
                     /translation="HCLSGSYMARSAKPRKRKPAPQRSKLPRYVVKLHPDDFFDEEDA
                     EVLHFDNFDDAVECCADLGIPFFLDAGNKKLVFWFVRVDDEGYPEIARCTEREFATIL
                     AGIRGLTLSGTKTHVKGFWS"
BASE COUNT         1391 a         1375 c         1368 g         1380 t
ORIGIN      
        1 agtccggtga tgaacagcac cactgtgtat accccatcag cgtggtcccg catgccatga
       61 tttttgtcac tgactcagtc accaccggac gcactgaacc ttccggctgt ttctccagcc
      121 agttaagcca gcggttaccc tgctgaaaaa tatcggcaaa acggggaagc atcagacggg
      181 tggggtaacg ccatccggcc agtgaactgt gccgcattcc gggcagtaca taccaccggc
      241 actgataggg gtctgacgct cagtggaacg aaaactcacg ttaagcaacg ttttctgcct
      301 ctgacgcctc ttttaatggt ctcagatgtc ctttggtcac cagttctgcc agcgtgaagg
      361 aataatggcc gagcatattg atatgtccgt ggcaaagcgg ggagaggcgt gcgatatctt
      421 catcattcag tgtttcaccc tgcgcccgga gatgatccag ggctgcctgc atataaatag
      481 tgttccataa cacgacggcg ttagtgacca gccccagtgt gcccagttga tcttcctgac
      541 cgtcggtata tcgttttctt atctcacctt tttgaccgtg acagatggct ctggcaacgg
      601 catggcgact ttctccccga ttaagctggg tcagaatgcg ccggcggtaa tcttcatcat
      661 caatataatt aagcagatac agcgttttgt tgatgcgccc cacttcaatg attgcctgag
      721 tcagtccgga aggacgttca cttttcagca atgaacggac cagcactgaa acctgtactt
      781 tgcccagctt cagggagcca gcggtccgga tcatttcgtc ccactgaagg actatttttc
      841 ggggatctga ttgccctctg gcaatatcat tcagcacgcc atagtcggca tcatggtcca
      901 ttcgccagaa aaccgaagca ccggcatcag ccaggcgtgg agaaaactgg tatcccagca
      961 gccagaaaag gccaaagaca agttcgctgg cacctgctgt atcggtcata atttcggttg
     1021 gattcagccc ggtctcctgt tccagaagac cttccagcac aaagatagag tccctcagcg
     1081 tccccggtat aacgatgcca tgaaagccgg aatactgatc ggacacaaag ttgtaccagg
     1141 tgatccctct gttattacca aagtatttgc ggttcggtcc ggcattgatt gttctgactg
     1201 gcgtaacaaa gcgcattcca tctgcagatg ccacttctcc tccaccccat atctgtgcca
     1261 gtggcagcgt tgcctgaaaa tcaaccagtc tggcattagc gctggtgata gtttcagccc
     1321 gcagatagtt cgcttttgtc cagttcagcc ggtgtcgggt cagtgcagga acatttgatc
     1381 tgatcagtgg ttccagaccg atattgcagg cttcagccat cagcacggcg ctgatgctga
     1441 cgggcagatc atcaactctg gcactggctt cactagcatg gaaaaactca tcagcaaatc
     1501 cggtatgggc gttaatttcg agcagcaact ccgttaaatc caccggaggg agtagatcac
     1561 tgatcatttt gctcagtcgt ttcagactgt ccggctcatc aagactggcg aggggagaaa
     1621 ttgtcaaccg gggcttcggg ccagaaacat cgagttcgac agcctcattt tcgcaaagac
     1681 gtgcagcaac ctgtctgtaa cgactatcaa gctgatgacc cagagatttt attgcttcct
     1741 gcgggtctgt cgggtgcccc aaagaacgat aaaccttaat ccggtttgcc tgccagtcag
     1801 caccctgtag taatcttgca cgaggatctc cccaccggtt actgccggta acgtagacat
     1861 ccctccgcct cagactatcc tgcagtttac tgagaaagca gagcgtgtat cccctgcggg
     1921 tgatatgttt ttccttgtta atcaccagcc gtttccatga ccgactgata atttccgttg
     1981 gtgcgtcgtc aaaaaactgc cgccgtgagc tgaactcccg gctgaggtag tcacaggcat
     2041 tcagagtggt aaccccggca ggtgcggatg aaaatttaac ggtattcagc agatggggca
     2101 ggaaacgacg aacgcgcccg tactgctcca ccatttcttc atgaaaatta tcgtctgagg
     2161 gccgggcaat ttcacggaca agcgtgatga tttcagccag cttttgcctt gggatgtagc
     2221 tgaacacctc agcacgaatc gattcgtccg gtgtttcttc tttcagcagg tacgaacatg
     2281 cgctggcgag cgccaatgca gatttatcca gatccttcag cgagcggagc cgttttttct
     2341 gcccaatctt tctggcgtca cggatgataa cggccagcat ggcgtccaga acgtccaatg
     2401 catcatccag cgccagcgtt tcccatgcaa ggacaaaggc aaccagaacc gccatccttt
     2461 tctgcggtga catcctggca atattgaaca ccgaagtcat accagcataa cgtgcgagat
     2521 ttttcaggcg cacagccggg agtgtactca ggttttcagc atgcaggcca aaatcgttca
     2581 gagttttcca gcgttcaatt gcttcattaa acgccggacc actgatggtc acagggccct
     2641 ttttcagtga ttccagtaaa gacaggcggc tgcaatcagt tggccccagc agcatctcca
     2701 gctgtgaacg ctgttcggct gacggtatca gtgccagttt gttccacagg cgcaacgtcg
     2761 ccttttccct tacctctgaa atcaaccggg tcagcgtagt ggctccgggg agaataatac
     2821 gatgttgcat aagccaccct gtcgccagat cgaaaagcag gccaggacgt tcgttgctta
     2881 tccagctccg ggtatataaa agacgggtaa ggcgaaatgt ccagggccag gcaaattcac
     2941 gatactgata gtgctgacgt atcagcgctg catgctcacg gcgggtattt tccctctgac
     3001 cgtattctgc aagaacggtg atatcacgaa tcccgagctg tctggcggta aaatgccgga
     3061 cgccggaagg aatatgattc atatcggtga ggaaggtgcc cagaaaacgg acacatccaa
     3121 tttgcagggc aatgcccaga cggttgtgat cacctctgct ttttccgata aattccttgt
     3181 ctgcttcatc aaggtgaaaa tatcgtgcca gctgaagctc atccggttca ccggtgaatc
     3241 tgccatagct ttcagtctgc tcagtggtca gaaagtcaac gggcatatcg gcctccctgc
     3301 ctgacgggca tttagtaaca tttttccaac cgtacgaaat gttataaatt atcggacatc
     3361 gtaaaactgt tacattaata tgtctattaa atcgtaaatt tgtaataata gacatgagtt
     3421 gtccgatatt cgatttaagg tacattttta tgcgactttt tggttacgct cgggtctcaa
     3481 ccagtcagca gtctcttgat cttcaggtca gagcactcaa agacgcaggt gtgaaagcaa
     3541 accgtatatt taccgataag gcatccggca gttcaacaga ccgggaaggg ctggatttgc
     3601 tgaggatgaa ggtggaggaa ggtgatgtca ttctggttaa gaagctcgac cgtcttggcc
     3661 gcgacactgc cgatatgatc caactgataa aggaatttga cgctcagggc gtggcagtcc
     3721 ggttcattga tgacgggatc agtaccgacg gtgatatggg gcaaatggtg gtcaccatcc
     3781 tgtcggctgt ggcacaggct gaacgccgga ggatcctaga acgcacgaat gagggccgac
     3841 aggaagcaaa gctgaaagga atcaaatttg gccgcaggcg taccgtggac aggaacgtcg
     3901 tgctgacgct tcatcagaag ggcactggtg caacggaaat tgctcatcag ctcagtattg
     3961 cccgctccac ggtttataaa attcttgaag acgaaagggc ctcgtgatac gcctattttt
     4021 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     4081 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     4141 gagacaataa ccctggtaaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     4201 acattttcgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     4261 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     4321 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     4381 tccaatgatg agcactttta aagttctgct atgtggtgcg gtattatccc gtgttgacgc
     4441 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     4501 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     4561 cataaccatg agtgataaca ctgctgccaa cttacttctg acaacgatcg gaggaccgaa
     4621 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     4681 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat
     4741 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     4801 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     4861 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     4921 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     4981 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     5041 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     5101 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     5161 ttaacgtgag ttttcgttcc actgagcgtc agacccctga taccggcaag aatggttgca
     5221 aactcccgct ccgtacatcg ggctatttcc ggataccctt cgtcatcgac acgaacaaac
     5281 cagaagacca gctttttgtt tcctgcatcc agaaagaacg gaatacccag gtcagcgcag
     5341 cactcaacgg catcgtcaaa attatcaaag tgcagaactt cagcgtcttc ttcgtcaaaa
     5401 aaatcatccg gatgaagttt cacaacatag cggggaagtt tgcttctttg tggtgcgggt
     5461 ttgcgtttac ggggtttagc tgaacgggcc atataactac ctgaaagaca atga
//
